LOCUS       DADMEQ010000001        96031 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.001, whole genome shotgun sequence.
ACCESSION   DADMEQ010000001 DADMEQ010000000
VERSION     DADMEQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 96031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.001"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            116..457
                     /locus_tag="JNZ05_000001"
     CDS             116..457
                     /locus_tag="JNZ05_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flxA-like family protein"
                     /protein_id="HAY8563381.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(448..1374)
                     /locus_tag="JNZ05_000002"
     CDS             complement(448..1374)
                     /locus_tag="JNZ05_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8563382.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1464..2462
                     /locus_tag="JNZ05_000003"
     CDS             1464..2462
                     /locus_tag="JNZ05_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HAY8563383.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2459..2677)
                     /locus_tag="JNZ05_000004"
     CDS             complement(2459..2677)
                     /locus_tag="JNZ05_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8563384.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2679..4694)
                     /gene="ligA"
                     /locus_tag="JNZ05_000005"
     CDS             complement(2679..4694)
                     /gene="ligA"
                     /locus_tag="JNZ05_000005"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAY8563385.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGSTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4765..5652)
                     /gene="zipA"
                     /locus_tag="JNZ05_000006"
     CDS             complement(4765..5652)
                     /gene="zipA"
                     /locus_tag="JNZ05_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HAY8563386.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPVSPQVAPAPQPVHSAPQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEA
                     VIIMNVAAHHGSELNGELLLNSIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKP
                     GTFDPEMKDFTTPGVTIFMQVPSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMT
                     PQKLREYQDIIREVKDANA"
     gene            5882..6643
                     /gene="cysZ"
                     /locus_tag="JNZ05_000007"
     CDS             5882..6643
                     /gene="cysZ"
                     /locus_tag="JNZ05_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HAY8563387.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6828..7799
                     /gene="cysK"
                     /locus_tag="JNZ05_000008"
     CDS             6828..7799
                     /gene="cysK"
                     /locus_tag="JNZ05_000008"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAY8563388.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8183..8440
                     /gene="ptsH"
                     /locus_tag="JNZ05_000009"
     CDS             8183..8440
                     /gene="ptsH"
                     /locus_tag="JNZ05_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HAY8563389.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8485..10212
                     /gene="ptsI"
                     /locus_tag="JNZ05_000010"
     CDS             8485..10212
                     /gene="ptsI"
                     /locus_tag="JNZ05_000010"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HAY8563390.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVAQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10253..10762
                     /gene="crr"
                     /locus_tag="JNZ05_000011"
     CDS             10253..10762
                     /gene="crr"
                     /locus_tag="JNZ05_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAY8563391.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(10804..11655)
                     /gene="pdxK"
                     /locus_tag="JNZ05_000012"
                     /gene_synonym="thiJ"
     CDS             complement(10804..11655)
                     /gene="pdxK"
                     /locus_tag="JNZ05_000012"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HAY8563392.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            11760..12134
                     /locus_tag="JNZ05_000013"
     CDS             11760..12134
                     /locus_tag="JNZ05_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HAY8563393.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            12167..12890
                     /locus_tag="JNZ05_000014"
                     /pseudo
     CDS             12167..12890
                     /locus_tag="JNZ05_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(13094..14005)
                     /gene="cysM"
                     /locus_tag="JNZ05_000015"
     CDS             complement(13094..14005)
                     /gene="cysM"
                     /locus_tag="JNZ05_000015"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HAY8563394.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(14139..15236)
                     /gene="cysA"
                     /locus_tag="JNZ05_000016"
     CDS             complement(14139..15236)
                     /gene="cysA"
                     /locus_tag="JNZ05_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HAY8563395.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(15226..16101)
                     /gene="cysW"
                     /locus_tag="JNZ05_000017"
     CDS             complement(15226..16101)
                     /gene="cysW"
                     /locus_tag="JNZ05_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HAY8563396.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(16101..16934)
                     /gene="cysT"
                     /locus_tag="JNZ05_000018"
     CDS             complement(16101..16934)
                     /gene="cysT"
                     /locus_tag="JNZ05_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HAY8563397.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(16934..17950)
                     /locus_tag="JNZ05_000019"
     CDS             complement(16934..17950)
                     /locus_tag="JNZ05_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8563398.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDEAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(18254..19045)
                     /gene="ucpA"
                     /locus_tag="JNZ05_000020"
     CDS             complement(18254..19045)
                     /gene="ucpA"
                     /locus_tag="JNZ05_000020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HAY8563399.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(19232..20233)
                     /gene="murR"
                     /locus_tag="JNZ05_000021"
     CDS             complement(19232..20233)
                     /gene="murR"
                     /locus_tag="JNZ05_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HAY8563400.1"
                     /translation="MFEPSVIIFSSCIGGDLTIYDQIIADVWNHISRIDRLKGIIEST
                     LAECEERHKNLSGIFAASPGEWKMLYLTKIRNAESEFTGNEQKIADFLRARVSELKSV
                     SSRQMAKQLGISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSIT
                     SDDSLEVIARKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGR
                     DLSFKLMKIGYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQG
                     ATVIAITSLADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            20196..21092
                     /gene="murQ"
                     /locus_tag="JNZ05_000022"
     CDS             20196..21092
                     /gene="murQ"
                     /locus_tag="JNZ05_000022"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAY8563401.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            21096..22520
                     /gene="murP"
                     /locus_tag="JNZ05_000023"
     CDS             21096..22520
                     /gene="murP"
                     /locus_tag="JNZ05_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HAY8563402.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAARACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(22700..23599)
                     /gene="yfeX"
                     /locus_tag="JNZ05_000024"
     CDS             complement(22700..23599)
                     /gene="yfeX"
                     /locus_tag="JNZ05_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HAY8563403.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMVL"
     gene            complement(23695..24270)
                     /locus_tag="JNZ05_000025"
     CDS             complement(23695..24270)
                     /locus_tag="JNZ05_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HAY8563404.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(24331..24780)
                     /locus_tag="JNZ05_000026"
     CDS             complement(24331..24780)
                     /locus_tag="JNZ05_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HAY8563405.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(24767..25192)
                     /locus_tag="JNZ05_000027"
     CDS             complement(24767..25192)
                     /locus_tag="JNZ05_000027"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HAY8563406.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLSPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            25406..26275
                     /gene="amiA"
                     /locus_tag="JNZ05_000028"
     CDS             25406..26275
                     /gene="amiA"
                     /locus_tag="JNZ05_000028"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HAY8563407.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            26279..27178
                     /gene="hemF"
                     /locus_tag="JNZ05_000029"
     CDS             26279..27178
                     /gene="hemF"
                     /locus_tag="JNZ05_000029"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HAY8563408.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(27184..28237)
                     /gene="eutR"
                     /locus_tag="JNZ05_000030"
                     /pseudo
     CDS             complement(27184..28237)
                     /gene="eutR"
                     /locus_tag="JNZ05_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(28322..28783)
                     /gene="eutK"
                     /locus_tag="JNZ05_000031"
     CDS             complement(28322..28783)
                     /gene="eutK"
                     /locus_tag="JNZ05_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HAY8563409.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGT"
     gene            complement(28796..29455)
                     /gene="eutL"
                     /locus_tag="JNZ05_000032"
     CDS             complement(28796..29455)
                     /gene="eutL"
                     /locus_tag="JNZ05_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HAY8563410.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(29465..30355)
                     /locus_tag="JNZ05_000033"
     CDS             complement(29465..30355)
                     /locus_tag="JNZ05_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HAY8563411.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVAANP
                     DVQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEIL
                     GAKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAV
                     IVDLAKRMLEQKASGINMTR"
     gene            complement(30376..31737)
                     /locus_tag="JNZ05_000034"
     CDS             complement(30376..31737)
                     /locus_tag="JNZ05_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HAY8563412.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAIAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDGCYTNHADSDQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            31956..33176
                     /locus_tag="JNZ05_000035"
     CDS             31956..33176
                     /locus_tag="JNZ05_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8563413.1"
                     /translation="MDAAQPKDKQYKLTASLTPGLFLLVHPNGSKYWRFRYWIDKKER
                     LQAIGVYPLISLKEARKRATESRLLIAQGIDPMDLARKEKAIDALNMAAGFKTVAEDW
                     LATRVGGWSESYTKQVKSALEKDVYPVLGKRSIVDITARDVLALLQKKERTAPEQARK
                     LRQRIGEIFKFAVITELVTRNPVADLDTALKARRPGHNAWIPISEIPAFYKALERAGS
                     VQIQAAIRLLILTALRTAELRLCRWEWINLEDATITLPAEVMKARRPHVVPLSRQAVE
                     LLQDQFTRSGYSVFVFPGRFMDKPLSASAILKALERIGYKSIATGHGWRTTFSTALNE
                     SGRYNPDWIEIQLAHVPKGIRGVYNQAAYLKMRRQMLQDYAHCRSARSNPHDKAVRIV
                     TIMLKDSLNHSLTN"
     gene            33641..34393
                     /locus_tag="JNZ05_000036"
     CDS             33641..34393
                     /locus_tag="JNZ05_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8563414.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIYTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCRE"
     gene            35153..35548
                     /locus_tag="JNZ05_000037"
     CDS             35153..35548
                     /locus_tag="JNZ05_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY8563415.1"
                     /translation="MNNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLDKQDNRIKNIRLMPKEK
                     YRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            35566..35829
                     /locus_tag="JNZ05_000038"
     CDS             35566..35829
                     /locus_tag="JNZ05_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8563416.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            35852..36394
                     /locus_tag="JNZ05_000039"
     CDS             35852..36394
                     /locus_tag="JNZ05_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8563417.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            36859..37065
                     /locus_tag="JNZ05_000040"
     CDS             36859..37065
                     /locus_tag="JNZ05_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8563418.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            37524..37862
                     /locus_tag="JNZ05_000041"
     CDS             37524..37862
                     /locus_tag="JNZ05_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY8563419.1"
                     /translation="MLDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMA
                     EPEGRCVRMLSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEP
                     DLRSCANMLA"
     gene            37843..38121
                     /locus_tag="JNZ05_000042"
     CDS             37843..38121
                     /locus_tag="JNZ05_000042"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8563420.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNIREYKNV"
     gene            38114..39574
                     /locus_tag="JNZ05_000043"
     CDS             38114..39574
                     /locus_tag="JNZ05_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8563421.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            39596..40417
                     /gene="murI"
                     /locus_tag="JNZ05_000044"
     CDS             39596..40417
                     /gene="murI"
                     /locus_tag="JNZ05_000044"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY8563422.1"
                     /translation="MRLNMFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNM
                     NAPYGNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEKYSGYAKSFPFPI
                     LSIIEPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLI
                     EEGNLNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAH
                     DQAISIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(40536..41230)
                     /locus_tag="JNZ05_000045"
     CDS             complement(join(40536..40984,40984..41230))
                     /locus_tag="JNZ05_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY8563423.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(41754..43127)
                     /gene="eutA"
                     /locus_tag="JNZ05_000046"
     CDS             complement(41754..43127)
                     /gene="eutA"
                     /locus_tag="JNZ05_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HAY8563424.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVSDLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVDVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVCATVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(43102..43581)
                     /gene="eutP"
                     /locus_tag="JNZ05_000047"
     CDS             complement(43102..43581)
                     /gene="eutP"
                     /locus_tag="JNZ05_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HAY8563425.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIVVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(43594..43929)
                     /gene="eutS"
                     /locus_tag="JNZ05_000048"
     CDS             complement(43594..43929)
                     /gene="eutS"
                     /locus_tag="JNZ05_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HAY8563426.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(44222..46501)
                     /gene="maeB"
                     /locus_tag="JNZ05_000049"
     CDS             complement(44222..46501)
                     /gene="maeB"
                     /locus_tag="JNZ05_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HAY8563427.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFVADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            46790..47740
                     /gene="tal"
                     /locus_tag="JNZ05_000050"
     CDS             46790..47740
                     /gene="tal"
                     /locus_tag="JNZ05_000050"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY8563428.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPLDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            47760..49763
                     /gene="tkt"
                     /locus_tag="JNZ05_000051"
     CDS             47760..49763
                     /gene="tkt"
                     /locus_tag="JNZ05_000051"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY8563429.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATCLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(49866..50873)
                     /locus_tag="JNZ05_000052"
     CDS             complement(49866..50873)
                     /locus_tag="JNZ05_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HAY8563430.1"
                     /translation="MLPTSLVWAAPAQRAFSDWQVTCNNQNFCVARNTGDHNGLVMTL
                     SRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDKWRISPWLLVTDD
                     TATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVGSETAWIKKGDEP
                     PLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNALRCSLDPLRREVNVTAL
                     TDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQETNELELMNATF
                     DEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNWHGPDARPTLWIT
                     R"
     gene            complement(51034..51609)
                     /gene="nudK"
                     /locus_tag="JNZ05_000053"
     CDS             complement(51034..51609)
                     /gene="nudK"
                     /locus_tag="JNZ05_000053"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HAY8563431.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(51677..53656)
                     /gene="aegA"
                     /locus_tag="JNZ05_000054"
     CDS             complement(51677..53656)
                     /gene="aegA"
                     /locus_tag="JNZ05_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HAY8563432.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDTPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            53862..55579
                     /gene="narQ"
                     /locus_tag="JNZ05_000055"
                     /pseudo
     CDS             53862..55579
                     /gene="narQ"
                     /locus_tag="JNZ05_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            55781..58894
                     /gene="acrD"
                     /locus_tag="JNZ05_000056"
     CDS             55781..58894
                     /gene="acrD"
                     /locus_tag="JNZ05_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HAY8563433.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFIHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSADFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNSYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGTQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(58993..59052)
                     /gene="ypfM"
                     /locus_tag="JNZ05_000057"
     CDS             complement(58993..59052)
                     /gene="ypfM"
                     /locus_tag="JNZ05_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HAY8563434.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            59433..59789
                     /locus_tag="JNZ05_000058"
     CDS             59433..59789
                     /locus_tag="JNZ05_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HAY8563435.1"
                     /translation="MVTLYGIKNCDTIKKTRRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLYAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            59793..60920
                     /gene="dapE"
                     /locus_tag="JNZ05_000059"
     CDS             59793..60920
                     /gene="dapE"
                     /locus_tag="JNZ05_000059"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HAY8563436.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            60948..61148
                     /locus_tag="JNZ05_000060"
     CDS             60948..61148
                     /locus_tag="JNZ05_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HAY8563437.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(61258..61956)
                     /gene="ypfH"
                     /locus_tag="JNZ05_000061"
     CDS             complement(61258..61956)
                     /gene="ypfH"
                     /locus_tag="JNZ05_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY8563438.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(62030..64045)
                     /locus_tag="JNZ05_000062"
     CDS             complement(62030..64045)
                     /locus_tag="JNZ05_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAY8563439.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRKLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(64060..64923)
                     /locus_tag="JNZ05_000063"
     CDS             complement(64060..64923)
                     /locus_tag="JNZ05_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HAY8563440.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRCSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(65091..65804)
                     /gene="purC"
                     /locus_tag="JNZ05_000064"
     CDS             complement(65091..65804)
                     /gene="purC"
                     /locus_tag="JNZ05_000064"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAY8563441.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVAHRLGVQLD"
     gene            complement(66017..67051)
                     /gene="bamC"
                     /locus_tag="JNZ05_000065"
     CDS             complement(66017..67051)
                     /gene="bamC"
                     /locus_tag="JNZ05_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HAY8563442.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPASLEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(67068..67946)
                     /gene="dapA"
                     /locus_tag="JNZ05_000066"
     CDS             complement(67068..67946)
                     /gene="dapA"
                     /locus_tag="JNZ05_000066"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAY8563443.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMARMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            68092..68664
                     /locus_tag="JNZ05_000067"
     CDS             68092..68664
                     /locus_tag="JNZ05_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HAY8563444.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            68664..69134
                     /gene="bcp"
                     /locus_tag="JNZ05_000068"
     CDS             68664..69134
                     /gene="bcp"
                     /locus_tag="JNZ05_000068"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HAY8563445.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            69387..70004
                     /gene="hyfA"
                     /locus_tag="JNZ05_000069"
     CDS             69387..70004
                     /gene="hyfA"
                     /locus_tag="JNZ05_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HAY8563446.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            70004..72021
                     /gene="hyfB"
                     /locus_tag="JNZ05_000070"
                     /pseudo
     CDS             70004..72021
                     /gene="hyfB"
                     /locus_tag="JNZ05_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            72032..72979
                     /gene="hyfC"
                     /locus_tag="JNZ05_000071"
     CDS             72032..72979
                     /gene="hyfC"
                     /locus_tag="JNZ05_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HAY8563447.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLLTLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGQGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            72996..74435
                     /locus_tag="JNZ05_000072"
     CDS             72996..74435
                     /locus_tag="JNZ05_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
                     /protein_id="HAY8563448.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISDYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            74447..75097
                     /gene="hyfE"
                     /locus_tag="JNZ05_000073"
                     /pseudo
     CDS             74447..75097
                     /gene="hyfE"
                     /locus_tag="JNZ05_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
     gene            75102..76682
                     /gene="hyfF"
                     /locus_tag="JNZ05_000074"
     CDS             75102..76682
                     /gene="hyfF"
                     /locus_tag="JNZ05_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HAY8563449.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            76672..78386
                     /gene="hyfG"
                     /locus_tag="JNZ05_000075"
                     /pseudo
     CDS             76672..78386
                     /gene="hyfG"
                     /locus_tag="JNZ05_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            78396..78941
                     /gene="hyfH"
                     /locus_tag="JNZ05_000076"
     CDS             78396..78941
                     /gene="hyfH"
                     /locus_tag="JNZ05_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HAY8563450.1"
                     /translation="MLKLLKTIMRAGTPTVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            78938..79696
                     /gene="hyfI"
                     /locus_tag="JNZ05_000077"
     CDS             78938..79696
                     /gene="hyfI"
                     /locus_tag="JNZ05_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HAY8563451.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            79689..80102
                     /gene="hyfJ"
                     /locus_tag="JNZ05_000078"
     CDS             79689..80102
                     /gene="hyfJ"
                     /locus_tag="JNZ05_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HAY8563452.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            80132..82144
                     /locus_tag="JNZ05_000079"
                     /pseudo
     CDS             80132..82144
                     /locus_tag="JNZ05_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
     gene            82166..83014
                     /locus_tag="JNZ05_000080"
                     /pseudo
     CDS             82166..83014
                     /locus_tag="JNZ05_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(83052..84113)
                     /locus_tag="JNZ05_000081"
     CDS             complement(83052..84113)
                     /locus_tag="JNZ05_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY8563453.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            84326..85789
                     /gene="bepA"
                     /locus_tag="JNZ05_000082"
     CDS             84326..85789
                     /gene="bepA"
                     /locus_tag="JNZ05_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HAY8563454.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVLQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLEQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            85810..86169
                     /gene="arsC"
                     /locus_tag="JNZ05_000083"
     CDS             85810..86169
                     /gene="arsC"
                     /locus_tag="JNZ05_000083"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HAY8563455.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(86302..87048)
                     /gene="hda"
                     /locus_tag="JNZ05_000084"
     CDS             complement(86302..87048)
                     /gene="hda"
                     /locus_tag="JNZ05_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HAY8563456.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(87098..88387)
                     /gene="uraA"
                     /locus_tag="JNZ05_000085"
     CDS             complement(87098..88387)
                     /gene="uraA"
                     /locus_tag="JNZ05_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAY8563457.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(88473..89099)
                     /gene="upp"
                     /locus_tag="JNZ05_000086"
     CDS             complement(88473..89099)
                     /gene="upp"
                     /locus_tag="JNZ05_000086"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAY8563458.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            89425..90462
                     /gene="purM"
                     /locus_tag="JNZ05_000087"
     CDS             89425..90462
                     /gene="purM"
                     /locus_tag="JNZ05_000087"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAY8563459.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGENYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            90462..91100
                     /gene="purN"
                     /locus_tag="JNZ05_000088"
     CDS             90462..91100
                     /gene="purN"
                     /locus_tag="JNZ05_000088"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAY8563460.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQSYAADE"
     gene            91272..93338
                     /gene="ppk1"
                     /locus_tag="JNZ05_000089"
     CDS             91272..93338
                     /gene="ppk1"
                     /locus_tag="JNZ05_000089"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HAY8563461.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFGHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            93343..94884
                     /gene="ppx"
                     /locus_tag="JNZ05_000090"
     CDS             93343..94884
                     /gene="ppx"
                     /locus_tag="JNZ05_000090"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HAY8563462.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLLRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(94923..>96031)
                     /gene="pdeF"
                     /locus_tag="JNZ05_000091"
     CDS             complement(94923..>96031)
                     /gene="pdeF"
                     /locus_tag="JNZ05_000091"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAY8563463.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPDSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        23162 a        25166 c        24253 g        23450 t
ORIGIN      
        1 aaaaaagcgc cctaaaggcg cttttttgct atctgcgatt tgtaagattg atttttcttc
       61 tcaagttcct tgttcttcta ccgatattac ctcgtacagt gacgaggact gaacaatggc
      121 cactatctct tcaacttcaa ttccttccat acaaacacag agtagtaaca gggcatcaca
      181 aggtagcgat gttgcttcac aaatcgcgcg gataagtcag caaatcatca aactcactca
      241 acaaattaaa gagattgtag acactagcgg cagtgctgaa gacaagcaaa aacaggccga
      301 attgattcaa cagcaaatca ccctactgga aacacaactg gcgcagttgc agaaacagca
      361 ggcagaaaag gcgcaggaga aagagcagcg tttgtctctg aatgtatccc ttctaaatcc
      421 tgttgaaaaa acgactcata tcgatatcta tatctgatac aacggatccc cttcccgacc
      481 agccattagc gcattgccct ggtctcgtac cacgtcccat aacgcttcag cggcggtgga
      541 aagtgaacgg ttcttcctgc gcaccagcat caactggcgt tcaaccaccg gcgtaatgcg
      601 cttaaccacc agtgggctac cttcaggcaa tggtagcgcc agtgcaggaa gaatactgat
      661 gccgatgcct gcggctacca tcgggaacag cgtcgccgga tgtccaatct cctgcacaat
      721 attcgcctga ataccattgc gcgccagcgc tgcgtcaatc agcggtcggc tgcctgacgc
      781 gtaatcctgc aacaccaatt tcgctccctg taatgcctgc cagggtacat aatcttccac
      841 ggccaaagcg ctatcgcgat ggcaaagcag aaagaaaggc tcggaaagaa tcgcttcaca
      901 ttgcagatcg cccactgggc ctggatcgat gacgatgcca aagtcgacat ctccctggcg
      961 aatactttcc atcacccact gctgcggacg atcatgcagg acaaactgaa tatctggata
     1021 gcggcgatgg ctttcagcaa tgcattgcgg aataagatgc gcggaaatgg tctggctggc
     1081 agcgacccga acttttccgc tcagttgttg ccccatacgc ccggtatcgc gcaacgtgct
     1141 gttaagttca tccagcagtc gctcaagacg caaagccaac tgctgccctg catctgtaag
     1201 caccacttca cgcgtggttc tgtccagcag gcgaacacca gtatgatttt ccagctcctt
     1261 cacactgtga cttactgccg actggctcaa gccgatacgc tctcctgcac gactaaaact
     1321 tttctcctgc gctactgtga cgaaaacctt taattgtttt aaagaataat tcatctgttt
     1381 tcttcatgaa tggatgcaat aaatcaattt tatttctcaa actggaagaa gcacaataga
     1441 accatcgatc atctggagtc tttatgaaac tttttcgtat cctcgatcct ttcaccttaa
     1501 ccctgatcac ggtggtgttg ctggcctctt tctttccggc cagaggcgat ttcgtcccct
     1561 tctttgaaaa tctgaccacc gcagctattg ccctgctgtt ctttatgcac ggcgcgaagt
     1621 tgtcgcgtga ggcgattatt gctggcggtg gtcactggcg actgcatttg tgggtaatgt
     1681 gcagcacctt cgtgctgttt ccgattctgg gtgtactgtt tgcctggtgg aaaccggtaa
     1741 atgtcgaccc gatgctctac tccggttttc tctacttgtg cattctcccg gctaccgtgc
     1801 agtctgcaat cgccttcacg tcaatggcgg gcggtaacgt cgcggcggcg gtttgttctg
     1861 cgtcagcatc cagcctgctg gggattttcc tttcaccatt gctggttggt ctggtgatga
     1921 acgttcacgg tgcaaggggc agccttgagc aggtcggtaa aattatgctg caactgctgc
     1981 tgccgtttgt gttggggcat ctttcccggc cgtggattgg tgactgggtg tcgcgcaata
     2041 aaaaatggat tgcgaaaact gaccagacgt ccattctgtt ggtggtttat acagcgttca
     2101 gcgaagccgt cgttaatggt atctggcata aagttggctg gggatcattg ctgtttatcg
     2161 tggtggtcag ctgcgttctt ctggctatcg tgattgtagt taacgtcttt atggcacgcc
     2221 gactgggctt caataaggca gatgaaatta ctatcgtctt ttgtggttcg aaaaagagtc
     2281 tggcaaatgg catcccgatg gcaaacattc tgttccccac atcggtgatc ggtatgatgg
     2341 tgctgcccct gatgattttc catcagatcc aattgatggt ctgtgcggtg ctggcgcgtc
     2401 gatacaaacg ccagaccgaa cagttacagg cgcagcagga aagcagcgcc gataaagctt
     2461 aaagcggacg cttcaggggc tggaccagtt gcgtcagccc ctcggtttta atcagcagcg
     2521 tgatttgcat tagctcaccg agcttccctg ccgggaactc atctttgcgg gcaaaccaca
     2581 gcagatactc ctccggcagg tcaattaagc gacgcccttt gtatttgcca aacggcatta
     2641 tcgtattggc tatttcaatc agctgctctt tttccatctc agctacccag caaacgcagc
     2701 atttccgctt cgtcgatgac ttcaatgccc agttcctgcg ccttcgccag tttagatcct
     2761 gcagcttcac ccgctatcac cagatcggtt ttcttcgaca cgctgcccgc gactttcgcg
     2821 cccagttcga ccagtcgagc tttagcgtca tcacgcgaca tctggcttaa gctgcccgta
     2881 agcaccacgg ttttaccagc aaacgggctg tcaatctctt ccgcgttgat aacgatcggc
     2941 gcaggccagt gaacaccttc cgccaacagc tcgctgatga cattgcggtt gctttcttcg
     3001 gcaaagaagt tgtgaacgtg ggatgcaacg acaatgccaa catcaggcac cttttgcagc
     3061 tcttcaatcg aagcggcttc cagcgcttcc agcgtgccga aatatgccgc cagacctgct
     3121 gcggtggcct cgccgacttc acggatgcca agtgcataga ggaagcgagc aaaggtggtt
     3181 tctttcgctt tttccagcgc gttaaccacg ttttgtgcca attttggccc catacgctcc
     3241 agtccggtca gtttgcctgc ggtgagtttg aacagatctg ccggagtatg gacatattct
     3301 ttttcaacca gctgatcgat gattttgtcg cccattccgt caacatccat cgcacggcgg
     3361 gaaacaaagt gtttcagcga ctctttacgc tgcgcaccgc aaatcaggcc accggtacag
     3421 cgggcaaccg cttcaccttc cacacgctca acgtcagaac cacataccgg acaatgcgtc
     3481 gggaatacaa cctcacgggt atcttccggg cgttcagaaa gcacgacgtt aaccacctgc
     3541 gggatcacgt cgccagcgcg gcgaatcacc actttatcgc caatgcgtaa accaagacgt
     3601 tcgatttcat ccgcattgtg taaggttgcg ttactcacca gcacgccagc aacatggaca
     3661 ggttccagac gcgcaacagg cgtaatcgcg ccagtacgcc caacctgaaa ctcgacgtca
     3721 cgcacaaagg tcatctgctc ctgcgccggg aatttaaacg ctaccgccca gcgcggggca
     3781 cgcgcgacaa agccaagctg ctcctgctgt tccagtgagt tgaccttaat caccacgccg
     3841 tcgatatcaa agcccagcgt cgggcggtct tcttccactt tgtgatagaa cgccagcact
     3901 tcttccgccg attcacaaag cgttacccga tcgctgaccg gcaaccccca ctttttaaat
     3961 tgcagtaaac ggccaagatg agtatccggc agctcgccac cttccagaac accaacgcca
     4021 tagcagaaaa aagtgagcgg ccgcttcgct gtaatacgcg gatcaagctg acgcagtgaa
     4081 ccagctgccg cattacgtgg gttagcaaaa actttcccgc ccgtgcgtcg cgcatcttcg
     4141 ttaatctttt cgaaccccgc ctgcggcagg aacacttcac cacgcacttc cagacgcgcc
     4201 gggatattct ctccgtgcag cttcagcgga atggcgcgaa tagtacgcac attagacgtg
     4261 atatcttccc cggtggagcc atcgccacgg gtcgcggcac tgactaaaac gccattttca
     4321 tacagaatac tgacggcaag gccatccagc ttcagctcac agcaccaggt gactttctcg
     4381 ttgtttttca gacggtcctg cacacgtttg ttgaaagcaa gaaagctttc ttcatcaaaa
     4441 acgttatcca gtgacagcat tggtacttca tggcgtatct ggctgaaagc tgccagcggc
     4501 gcagcaccta cacgttgggt aggcgaatca ggcgtaatca gttctggatg tttggtttcc
     4561 agctcgcgca gttcgcgcat cagcctgtcg tattcagcgt cgggaatttc cggcgcatcc
     4621 atcacatgat aaagatattc atgatggcaa agcgtcgttc gcagttctgt cagttgttgt
     4681 tcgattgatt ccatatcgca ccatcaatgc taaaaacccc cgacaagcgg gggttcgaag
     4741 aggagttaat ttgccttaag tgtatcaggc gttggcgtct ttgacttcgc ggatgatgtc
     4801 ctggtactcg cgcaatttct gcggagtcat catacggcgc tgatcgtcaa gcacgacacc
     4861 gcccacttca tcggcaatat gctgcgcaga ttgcagcatc agcttgaagt tctgcagctc
     4921 gtcaccgtaa gacggtacct gcataaagat agtgacaccc ggagtagtga aatccttcat
     4981 ttcaggatca aaggttcccg gtttcaccat attcgccagg ctgaataacg ccgggccgct
     5041 gccatccggg ctaagatgac gatggtaaat attcatatcg ccaaaaatga agcccgcttg
     5101 ttgaatgctg ttaagaagca gttcaccgtt tagctcgcta ccgtgatgcg ccgcgacgtt
     5161 catgataatc accgcttctt tgcgcttcgg tttatccata actggagccg gttccgctac
     5221 aggctcaggc tgtggtgccg ctacgggttc tgcaggctgg aaagcctgtt gtgccggttg
     5281 cggtgctgaa tgcactggct gcggcgctgg cgcgacctgt ggcgaaactg gctgctggac
     5341 cggttgacgc ggctgcgcag acgcataagg cggttggtac tggtgttgcg gcgacggacg
     5401 agcagcctca tgctcctgag cgttagccgg ggcatgattc acgcggtgaa cacgaacctc
     5461 accaacgccc tcatcatctt cgacatcctc gtcataagaa tcgtcgtcac gttttgactt
     5521 cattcgtttt aatggccgat cgcggaacat agaagatcgt tctttacggc tggtccagaa
     5581 accatgtacc agtaaagcga ttatggcgat cgcgccaaca atgattaata tcagacgcaa
     5641 atcctgcatc attatattct ctgttgttct aacaccttgc caccacggca aacatttact
     5701 cactaagagt atttgccgat tacctcaagt gcaagtgcac tattaacttt cacagcacaa
     5761 agatagatga aatcgtgctt tttgctgttt tttcgaacat atcctaactg tccattgcgc
     5821 aattacccgg taaaatacgc agaattttcc tgggattggt caaaaggagc tcatcctgat
     5881 tatggtttca tcattcacat ctgccccacg cagcggtttt tactattttg cgcaaggctg
     5941 gaagctcgtc tcgcaacctg ggattcggcg tttcgttatt ttaccgctgc tggtcaatat
     6001 tttgttgatg gggggcgcat tctggtggct ctttacacag ctcgatgtct ggatcccgac
     6061 tttcatgagt tacgttccgg actggctaca atggctgagt tatctgttgt ggcctctggc
     6121 tgtcatctct gtgctgttag tgtttggcta tttcttctcc acgattgcta actggattgc
     6181 cgctccgttt aacggtttat tggctgaaca actggaagca cgattgactg gcgctacacc
     6241 gccagatacc gggattttcg gtatcatgaa agatgtgccg cgaatcatga aacgcgaatg
     6301 gcaaaaattt gtctggtatc tgccgcgcgc aattgtatta ctaattcttt acttcatccc
     6361 tggtattggg caaaccgtcg cgccggtact gtggttcctg tttagcgcct ggatgttagc
     6421 catccagtat tgcgattacc ccttcgataa ccacaaagtg ccgtttaaag agatgcgcac
     6481 cgccctgcgc acacgcaaaa tcaccaatat gcagtttggt gctttaacca gcctgtttac
     6541 gatgatcccg ctgcttaatc tgttcatcat gcccgttgcc gtttgtggcg cgacggcgat
     6601 gtgggtcgat tgctatcgcg ataaacacgc gatgtggcgg taacaatcta ccggttattt
     6661 tgtaaaccgt ttgtgtgaaa caggggtggc ttatgccgcc ccttattcca tcttgcatgt
     6721 cattatttcc cttctgtata tagatatgct aaatccttac ttccgcatat tctctgagcg
     6781 ggtatgctac ctgttgtatc ccaatttcat acagttaagg acaggccatg agtaagattt
     6841 ttgaagataa ctcgctgact atcggtcaca cgccgctggt tcgcctgaat cgcatcggta
     6901 acggacgcat tctggcgaag gtggaatctc gtaaccccag cttcagcgtt aagtgccgta
     6961 tcggtgccaa catgatttgg gatgccgaaa agcgcggcgt gctgaaacca ggcgttgaac
     7021 tggttgaacc gaccagcggt aataccggga ttgcactggc ctatgtagct gccgctcgcg
     7081 gttacaaact caccctgacc atgccagaaa ccatgagtat tgaacgccgc aagctgctga
     7141 aagcgttagg tgcaaacctg gtgctgacgg aaggtgctaa aggcatgaaa ggcgcaatcc
     7201 aaaaagcaga agaaattgtc gccagcaatc cagagaaata cctgctgctg caacaattca
     7261 gcaatccggc aaaccctgaa attcacgaaa aaaccaccgg tccggagatc tgggaagata
     7321 ccgacggtca ggttgatgta tttattgctg gcgttgggac tggcggtacg ctgactggcg
     7381 tcagccgcta cattaaaggc accaaaggca agaccgatct tatctctgtc gccgttgagc
     7441 caaccgattc tccagttatc gcccaggtgc tggcaggtga agagattaaa cctggcccgc
     7501 ataaaattca gggtattggc gctggtttta tcccggctaa cctcgatctc aagctggtcg
     7561 ataaagtcat tggcatcacc aatgaagaag ccatttctac cgcccgtcgt ctaatggaag
     7621 aagaaggtat tcttgcaggt atttcttctg gagcagctgt tgccgcagcg ttgaaactac
     7681 aagaagatga aagctttacc aacaagaata ttgtggttat tctaccatca tcgggtgagc
     7741 gttatttaag caccgcattg tttgccgatc tcttcactga gaaagaattg caacagtaat
     7801 gccagcttgt taaaaatgcg taaaaaagca cctttttagg tgcttttttg tggcctgctt
     7861 caaactttcg cccctcctgg cattgattca gcctgtcgga actggtattt aaccagacta
     7921 attattttga tgcgcgaaat taatcgttac aggaaaagcc aaagctgaat cgattttatg
     7981 atttggttca attcttcctt tagcggcata atgtttaatg acgtacgaaa cgtcagcggt
     8041 caacacccgc cagcaatgga ctgtattgcg ctcttcgtgc gtcgcgtctg ttaaaaactg
     8101 gcgctaacaa tacaggctaa agtcgaaccg ccaggctaga ctttagttcc acaacactaa
     8161 acctataagt tggggaaata caatgttcca gcaagaagtt accattaccg ctccgaacgg
     8221 tctgcacacc cgccctgctg cccagtttgt aaaagaagct aagggcttca cttctgaaat
     8281 tactgtgact tccaacggca aaagcgccag cgcgaaaagc ctgtttaaac tgcagactct
     8341 gggcctgact caaggtaccg ttgtgactat ctccgcagaa ggcgaagacg agcagaaagc
     8401 ggttgaacat ctggttaaac tgatggcgga actcgagtaa tttcccgggt tcttttaaaa
     8461 atcagtcaca agtaaggtag ggttatgatt tcaggcattt tagcatcccc gggtatcgct
     8521 ttcggtaaag ctctgcttct gaaagaagac gaaattgtca ttgaccggaa aaaaatttct
     8581 gccgaccagg ttgctcagga agttgaacgt tttctgagcg gtcgtgccaa ggcatcagcc
     8641 cagctggaaa cgatcaaaac gaaagctggt gaaacgttcg gtgaagaaaa agaagccatc
     8701 tttgaagggc atattatgct gctcgaagat gaggagctgg agcaggaaat catagccctg
     8761 attaaagata aacacatgac agctgacgca gctgctcatg aagttatcga aggtcaggct
     8821 tctgccctgg aagagctgga tgatgaatac ctgaaagaac gtgcggctga cgtacgtgat
     8881 atcggtaagc gcctgctgcg caacatcctg ggcctgaaga ttatcgacct gagcgccatt
     8941 caggatgaag tcattctggt tgccgctgac ctgacgccgt ccgaaaccgc acagctgaac
     9001 ctgaagaagg tgctgggttt catcaccgac gcgggtggcc gtacttccca cacctctatc
     9061 atggcgcgtt ctctggaact gcctgctatc gtgggtaccg gtagcgtcac ctctcaggtg
     9121 aaaaatgacg actatctgat tctggatgcc gtaaataatc aggtttacgt caatccaacc
     9181 aacgaagtta ttgataaaat gcgcgctgtt caggagcaag tggcttctga aaaagcagag
     9241 cttgctaaac tgaaagatct gccagctatt acgctggacg gtcaccaggt agaagtatgc
     9301 gctaacattg gtacggttcg tgacgttgaa ggtgcagagc gtaacggcgc tgaaggcgtt
     9361 ggtctgtatc gtactgagtt cctgttcatg gaccgcgacg cgctgcccac tgaagaagaa
     9421 cagtttgctg cttacaaagc agtggctgaa gcgtgtggct cgcaggcggt tatcgttcgt
     9481 accatggaca tcggcggcga caaagagctg ccatacatga acttcccgaa agaagagaac
     9541 ccgttcctcg gctggcgcgc tatccgtatc gcgatggatc gtaaagagat cctgcgcgat
     9601 cagctccgcg ctatcctgcg tgcctcggct ttcggtaaat tgcgcattat gttcccgatg
     9661 atcatctctg ttgaagaagt gcgtgcactg cgcaaagaga tcgaaatcta caaacaggaa
     9721 ctgcgcgacg aaggtaaagc gtttgacgag tcaattgaaa tcggcgtaat ggtggaaaca
     9781 ccggctgccg caacaattgc acgtcattta gccaaagaag ttgatttctt tagtatcggc
     9841 accaatgatt taacgcagta cactctggca gttgaccgtg gtaatgatat gatttcacac
     9901 ctttaccagc caatgtcacc gtccgtgctg aacttgatca agcaagttat tgatgcttct
     9961 catgctgaag gcaaatggac tggcatgtgt ggtgagcttg ctggcgatga acgtgctaca
    10021 cttctgttgc tggggatggg tctggacgaa ttctctatga gcgccatttc tatcccgcgc
    10081 attaagaaga ttatccgtaa cacgaacttc gaagatgcga aggtgttagc agagcaggct
    10141 cttgctcaac cgacaacgga cgagttaatg acgctggtta acaagttcat tgaagaaaaa
    10201 acaatctgct aatccacgag atgcggccca atttactgct taggagaaga tcatgggttt
    10261 gttcgataaa ctgaaatctc tggtttccga cgacaaaaag gataccggaa ctattgagat
    10321 cattgctccg ctctctggcg agatcgtcaa tatcgaagac gtgccggatg tcgtttttgc
    10381 ggaaaaaatc gttggtgatg gtattgctat caaaccaacg ggtaacaaaa tggtcgcgcc
    10441 tgtagacggt accattggta aaatctttga aaccaatcac gcgttctcta tcgaatctga
    10501 tagcggcgtt gaactgttcg tccacttcgg tatcgacacc gttgaactga aaggcgaagg
    10561 cttcaagcgt attgctgaag aaggtcagcg cgtgaaagtt ggcgatactg tcattgaatt
    10621 tgatctgccg ctgctggaag agaaagccaa gtctaccctg actccggttg ttatctccaa
    10681 catggacgaa atcaaagaac tgatcaaact gtccggtagc gtaaccgtgg gtgaaacccc
    10741 ggttatccgc atcaagaagt aattcttgcc gcagtgaaaa atggcgccca tcggcgccat
    10801 tttttatgct tccgccagcg gcggcaaaat caattcatcg ctctcatgct gctgggtgta
    10861 gcgcatcact tccagtacgc gcaaccccgc acggtgcaca gcatcgttta acgccttccc
    10921 tttcagcaaa ccactgataa gctgagcaca aaacaggtcg ccagtccctt tcaggtcggt
    10981 ttttacccgt gaatgggaaa tgacattcac gctgtcggca ctgaccacca ccacctgcat
    11041 ctcctgattt tcttcattac cggaggcgct ggtaatcacc acccatttta atgtgtctga
    11101 aagcagactt tttgcggcag caatggcact gtcgagatcg cggcaatttt taccggtcag
    11161 gatttccagc tcaaagatat tgggggtaat tccctgcgcc agcggcagta aatattgtcg
    11221 atacgcctcg ggaaggtcag gtttgacata aattccgcta tcaatatcgc caatcaccgg
    11281 atcgaccatg atcaataggt caggatggtc tttgcgtagc gcagtcagcc actcggcaag
    11341 gattttgatt tgcgatgccg ttcccatata gcccgtggtt acagcacgaa gttggcgcag
    11401 cgcatcacgc tcctgaagcg cacgcaaata gccgctaaac cattcgtccg gaatcgcacc
    11461 accgtagaaa gtgtcataat gcggcgtatt gctcagcaat accgtcggca cggcaaagac
    11521 attcaagccg ttctgtttta tagcaggcac cgcaatgctg ttgcctacgc ttccgtacac
    11581 tacctgcgac tgcacggcga cgatatccgc ctgcagcgcc ctactcttat cgttaaacaa
    11641 caacaaacta ctcatttaat tttttctcct tgccgatgat cctcatcgta ttccaaccga
    11701 aactttacct gattctggca gtcaaatcgg ctattacaaa acaaagataa ggtaattcag
    11761 tgaagaaaat catttgtctg gccattacgc tgctaatgac actccccgct tacgcgaagt
    11821 taactgttca tgaagaagcc cgcatcaacg ccatgctgga gggattagca cagaaaaagg
    11881 atttgatatt tgtgcgcaac ggtgatgaac atacctgcga tgaagcggtt tcccacctgc
    11941 gtctgaagct cggcaatacc cgtaaccgca ttgacactgc cgagcagttt attgataagg
    12001 tagcttcgtc gtcatcgatt accgggaagc cgtatatcgt gaagatcccc ggtaagagcg
    12061 atgagaacgc acagcctttt ttacatgcgt taattgcgca gacggataaa acggtgcctg
    12121 cggaaggaaa ttaatccgct ttggtaaggc atttacagga ggtaacatga aaaaatgctt
    12181 tatttatcac gatgaaaaat cgaataaatt ttggtggata gattacgaag gggatagttt
    12241 agctgtcaac tatggcaagg taggtagtat tggtaaattc catacaaaag aattcgataa
    12301 tgaagaacag tgtctgaaag aagccagtaa attgattgcc gcaaaaatga aaaaaggata
    12361 tcaagaaact ccagacttta acttcatgga tctctactat tttgatgatg aagagattgg
    12421 gttacatctt aaaacgtcac acccaaactt ccagtgccat tttactgatc cactttatat
    12481 gtgttgctgg gatgaagaat ctccttttgg tagcgatgaa ggtgctgatg ctctaaacga
    12541 acttgaaaat agcctccgtt ctggactgtg ctgatttccc tcaaatgtta attgaaacta
    12601 tatggggtat gaaatacatc gctatggaca gtattcttga agaggatgtg cgtgcgcaat
    12661 tactagccga tgaaatgagc tctatccaga gcaatatgat tacctacgcg actgcattcg
    12721 gtcagatcaa agtcatgggt aaaatctccc ataaacttaa aaagatggga ctcaatgcac
    12781 tagcgcgtca tcagcttacc gcaaaaattc ttcaatgggg tgacggtcag gactcaccaa
    12841 tacttcaaaa aatgattgat gaccttactg cttttccaca cgaaaattaa aaactacatt
    12901 tgtcggcagc aacaactgcc gacaaatcag attattgaca aagtgcactt tgtttatgcc
    12961 ggatgcggcg taaacgcctg atccggcata aaaatattgc taattcaata tattgcagaa
    13021 acattgtagg cctgataagc ggagcgcatc aggcagtttt gcgtttgtca tcagtctcca
    13081 atgctattaa tccttaaatc cctgccccct gactaaaatg ctcttcccca aacactccgg
    13141 tggagaggta gcgatcgcca cgatcgcaga tgatcgctac caccaccgca ccagggttag
    13201 cttttgccac ccgcagtgct ccagcaaccg cgccgcctga gctgacgcca cagaatattc
    13261 cttcccgcac cgccagttcg cgcatggtgt tttccgcatc gcgctgatga atatccagca
    13321 cctcatccac cagcgcagcg ttgaaaatcc ccggcagata ttccgcaggc cagcggcgaa
    13381 tgcccggaat actgctgcct tcttccggtt gcaggccaac aatggtcacc ggtttggatt
    13441 gttcgcgcat aaagcgtgaa acgccggtga tagtgccggt cgtccccatg ctggagacaa
    13501 aatgagtgat gcgcccgccg gtttgctgcc agatttccgg cccggtggtg gtgtaatgcg
    13561 cataagggtt atcgggatta ttgaactgat cgagcagctt tccttcgcca cgattcgcca
    13621 tctccagcgc cagatcgcgc gcaccttcca tgccctgctc tttggtgaca agaatcagtt
    13681 ccgcaccata agcacgcatc gccgcacggc gttcctggct catgttgtcg ggcatcagca
    13741 atttcatgcg atagcctttc agcgcggcaa tcattgccag cgcaatgccg gtgttaccac
    13801 tggtggcttc gattaagaca tcacccggtt taatttcccc gcgcttttcc gcctcgacga
    13861 tcatcgaaag tgccgcacga tctttcaccg aacctgccgg gttattgcct tccagtttta
    13921 accacacttc actgccgtta tccggcccca ttcgctgcaa cttcaccaga ggcgtattgc
    13981 ctattgtttg ttctaatgta ctcacgatct ctatccatac gtggtgttgc ctgatgcgac
    14041 gcttgcgcgt cttatcaggc ctacaggtta caaaccttgc cattaaaaag cccggatcgc
    14101 gggaagcgcc tccgggcgtt taacattcac tcaacctatc aggcgctttg tgcgagagca
    14161 agttcctcat cgcgggtttc aatacgctcg tcgccgttat acagccgcgc atgttgcaga
    14221 ccaacgaata aacgctcgcc acgctgcggg gcatcgtcgc catgcatcac gaccgtcagc
    14281 ggttcgttgt accaccccag cggctgcacc actaattggg tgtagtgacc tttcgggctg
    14341 gcttccagta cctgtaccgg cagcggcgaa tcgagactgg tacggcggct gatatccact
    14401 tcccacgggc gcaggaagag atccaccggc ccctgatacg caggtgtgta gcccagcggc
    14461 cagcgatgcg cgccaacatg gaactgcccg ccgcgaatgg ttccctgcag gcggttcact
    14521 tcgcccataa attcgagtac aaaacgggtc gctggttcgc gccatacctg attcggcgca
    14581 tcagcctgtt caatattccc ctgactcatc accactacac gatcggctac ttcggtcgcc
    14641 tcttcctggt cgtgggtcac aaacacgctg gtgaatttca gctcttcatg cagttgacgc
    14701 agccagcgac gcagctcttt acgcacctgc gcatccagcg cgccaaacgg ttcatcaagc
    14761 agcagaattt gcggttctac agccagcgcg cgcgccagcg ccacacgctg tttctggccg
    14821 ccggaaagct gcgccggata acgatccgcc agatgcgcaa gctggaccat ttccagcaat
    14881 tttgtcactt tcgctttgat ggctgcggca ttcgggcgct cgcgacgcgg cagcaccgtc
    14941 aggccaaaag tgatattgtc gaataccgtc atatggcgga acagcgcgta atgctggaac
    15001 acgaaaccga ctttacgatc gcgcgcgtgc aggcgactca cgtcggtgcc gtggaagcga
    15061 atatgcccgc tggtttgatg ctccagcccg gcgataatcc gcagcagcgt ggttttcccg
    15121 gaaccggacg gccccagcaa cgcgaccatc tgaccagaag gaatatccag tgagatatcg
    15181 ttcagcacct gggtgcgacc aaaagacttc ttgatattgg caatctcaat gctcatgatg
    15241 ctcctcctgc tgcgcgcgtt tttcctgatt ctccaggcgc cactgcaaca tactctttaa
    15301 aaacagggtg ataatcgcca tcagcgtcaa cagcgcggcg gcggtaaagg agccgacggt
    15361 gttgtagtcc tgctccaaca attcaatctg taacggcagc gaaagtgttt cgccgcgaat
    15421 cgagccggaa accaccgaca cagcaccaaa ctcgccaatc gcgcgggcgt tggtcagcac
    15481 cacgccgtaa agcagcgccc agcgaatatt cggcaatgtg acgcgacgga acatctgcca
    15541 gccggacgcg ccaagcaaaa tcgccgcttc gtcttcctgg ctgccctggc ttaacatcac
    15601 cggcaccagc tcgcgcacca caaacggaca cgtcacaaag atggtgacca gcaccattcc
    15661 gggccaggag aacataattt gcagattatg ttcatccagc cagccgccga gcgggccgtt
    15721 ggagccgtag aacagcagat acaccagacc ggcaaccacc ggcgatacgg caaacggaat
    15781 gtccagtagc gtcagcagta actggcgtcc cgggaagtta aagcgcgtca ccagccaggc
    15841 cagcagaatg ccgaacacca ggtttaccgg tacggcaatc agcgcgatca tcaccgtcag
    15901 ccagatggcg tgcagcatgt ccggatcggc cagattctgt aaaaccggca tcagcccctt
    15961 gctgaatgcc tgcacgaaga tgtaaatcat cggcaccagc aggatgaacg ccgaaaccag
    16021 catcccgatg ccaatcagaa accatttgcc ccagttaatc gggcgcgcgt cataacgctt
    16081 caattgggta acttccgcca ttaatgacct accacacgcc gaccaaagcg actttgcaga
    16141 gtgttaattg agaacagcag cagcagagat gccgcgagga tcaccgaagc aatcgcgctc
    16201 gctgccgggt aatcaaactc ctgtaagcgc acaaaaatca tcagcgacgt cacttccgtc
    16261 ttccacgcga tatttccggc gataaaaatc accgcgccaa attcaccaag actacgggta
    16321 aacgacagcg ccacgcccgc caccagcgcc ggagaaagct ccggcagcac cactttgcag
    16381 aaactctgcc agcgcgttgc accaagcgtt tccgccgctt cttcatattc cgggcctaac
    16441 tcttccagca ccggctgcac ggtacgcacc acaaacggaa tgctggtaaa ggccatcgcc
    16501 accgcaatac caagccaggt ataggtgact ttgatatcaa acttcgccag ccattcaccg
    16561 taaaaaccgt ttacggaaaa gagcgaggcc agcgttaaac cggcgactgc cgttggcagc
    16621 gcaaagggta aatccatcag cgcatcaagc agcgtgcggc ccggaaagcg ataacgtgtt
    16681 aaaatccacg ccatcagcag accgaaaacg ccgttaaaaa tcgatgccac aaacgccgac
    16741 agcagcgtca ctttgtaggc cgcgaccacc tgcgggttgg tgatcacctc ccagtactgc
    16801 gcccagctca tctcagaaag ctgcatcacc agcgcagaga gcggcagcag caatatcagg
    16861 cacacaaaca gcagactggt gccaaggctt aaagtaaagc ccggcagcac gcgtcttgaa
    16921 gaaactgcaa acattactta cgccccgccg ctaacagctt gtctaactcg ccgccgctgg
    16981 tgaagtgggt tttcatcact tccggccatg agccaaattt gtcttccacg cggaacagtt
    17041 cggtctgcgg gaatttatct ttcagtttgt ccatgacttc cgggttattc acacggtagt
    17101 aatagtcggt gatgatggtc tgcgcctgtg ggctgtagag ccagttcagg taggcttttg
    17161 cggctttttc cgtaccgttg gcctgcacat ttttatcgac ccacgccacc gggaattccg
    17221 ccagaatatt ggttttcggg atcaccactt caaagccctg tgcttcatac tgtttacgga
    17281 tgttgttcac ttccgattca aagctaatca gcacatcgcc caagccacgc tcggcaaaag
    17341 tggtggttgc gccacgaccg ccggtatcga acacttcaac gtttttcagg aactgggtca
    17401 taaactgttc ggttttggct tcatcgccac cgtcagcttt atccgctgcg ccccatgccg
    17461 ccagataggt ataacgcgcg ttacccgacg ttttcgggtt cgggaaaatc agcttcacgt
    17521 cggagcgcac caggtcgttc cagtcgtgga tattcttcgg gttgccctta cgcaccagga
    17581 agcccatggt ggagtagaac ggcgagctat tattcggcag gcgcgactgc cagtcagccg
    17641 ggatcagctt gcctttgtcg tgcaggattt gtacgtcggt cacctggtta taagtgacaa
    17701 cgtctgcttt taagccctgc aaaatcgcca gcgcctgttt tgatgacccg gcatgagatt
    17761 gttttatcgt cagtttgtcg ccgccgttat cttttgccca ttgctgttca aacggcggat
    17821 tcagggcggc aaacagctcg cgggagacgt cataagaact gttcagcagt tccgttgcct
    17881 gtacatggcc cgccagcagc agagaagcga ccagcgcgag tgagttcttt ttcagtaagt
    17941 taacggccat tgcgcaccct tataaattta atgactttct aatagccatc atatttataa
    18001 cggacgtgaa aggagtaacg gttttatata ccgtttggtg atttggaagt taaaaaggga
    18061 ataagaactt ggttcaacag gcgtaattgt tgcaggcagt ttggacacag acagcgcgga
    18121 gaaaccggag cgtacacata gtacgtgagg atttcgagca ctgcccgggg ccaaaatgac
    18181 aaatgaaata gcctggtgaa cttagttcaa gacgaaatcc tccccacaca tgcagggagg
    18241 aaacagaggt gaatcagata ccgacgctaa ccgtctccgg cagtgtgctg ccgccatcaa
    18301 tcacattctg tgtaccggtt aaatagctgg attcatccga tgcgaggaag gccgccagtt
    18361 cgccgacttc cagcggatcg gcgaggcgac acagcgggat tgcttttgcc atttcagtca
    18421 gcaccgattc tggatcttcc gggttcgact ggcgggcaat gctttccgcc attggcgtgc
    18481 ggacgtatcc cgggcagatg gcgttaacgc gaataccgga ttgcgcgtac tccaccgcca
    18541 gcgatttagt caggccaaca atcgccgctt tcgttaaggc atacgccgtt tcgccaggat
    18601 cggccaccat atcaccagtg actgaagaca tcatcacaat gcgaccatct ttgcgcgcaa
    18661 tcatctccgg cagcaccgcc ttcgtgacgt tccatacgcc tttaatattg atatcaatat
    18721 ggaaatcgcg atcttcatcg ctcatatcga ggaaactgcc cagacgacaa acgcctgcgt
    18781 tattcactag gatatcaatg cgcccttctt tttccttcgc gcgtttgata gctgcggcta
    18841 ccgacgccgg gtcacgcaca tcggcgacaa ccgccgtaca gcgatgacca cgaccacaca
    18901 gttcgtccgc tagcttttcg atctcagggg agatatccag caagattagg ttcgcgccat
    18961 gacgtgcaaa agttctggca attccttcgc caattccctg caatgcgccc gtaatcagtg
    19021 ctgtcttgcc cgtgagttta cccattttaa tgctctcctt gttaatacag agcgttacac
    19081 tccattccct ctgaaaatac aggataaaca aaatattgct gcggatcagc tcactaattc
    19141 aggaaaatct caatccagtc gctgctttat gcatcatttc aggcgttgtg ttagctcact
    19201 gctgcgctga atcattttca atgacgccac atcattgatg ttgcacaaga ccaacaaaca
    19261 gtaaatcggt aacagaattt tgcgccgtgc gggtagacat cgacgagcta cgccattcgg
    19321 tttcaccgga aacggtatcg agcgtgaaat gcgccagccg ccgcagcgga gagtcagcca
    19381 gagaagtaat ggcaatcacc gttgccccct gctttcgtgc cgcttctgcg cacaagacaa
    19441 tctctttttt actgccgctg taagagatag cgatttgtac atcgcctttt ttcaacgcct
    19501 gggaaacggt ggcttgtacg tgcgtgtcgg cttcgcaggc cacgcgatag ccaattttca
    19561 tcagtttgaa cgacagatcg cgccccacca gcgccgatcc gcccaggccc gttatctgga
    19621 taaacggtgc tttactgatg acctcaatga ttttttgcag ccgcgcgtag tcgaacaacg
    19681 cacaggtttg ctccagcgcc agctcttttt cgcgattcag tttgcgggca atcacttcca
    19741 gcgaatcatc actggtaatc gaactgtgca ggtgcagcgc cgtggcattc gttttctccc
    19801 ggctggcact gtattcgcca attaacgcca tacgcaattc agtaaatcct tgcgcgccga
    19861 gcttctgggc aaatttcaca atgctcgact ggctgatacc gagctgtttc gccatctgcc
    19921 gtgaagagac ggatttcagc tcgctcactc tggcccgcag aaagtcagca attttttgct
    19981 cgttcccggt aaattcactt tccgcattgc gtattttcgt caggtacaac atcttccact
    20041 ctcctgggct ggcagcaaaa attccgctta agttcttatg acgctcttca cactctgcga
    20101 gtgtagactc aatgattcct ttaagtctgt caattcggga tatatgattc catacatctg
    20161 caattatttg atcgtaaata gtaaggtcac caccgatgca actagaaaag atgattactg
    20221 aaggctcgaa caccgcctcg gctgaaattg accgcgtatc gacgctggaa atgtgccgga
    20281 ttatcaacga tgaagataaa accgtaccgc ttgccgttga tcgcgtcctg ccggatatcg
    20341 ccgcggcgat cgatgttatc cacgcccagg tcagcggcgg cggtcgtctg atttatctcg
    20401 gtgcgggaac atccggtcgt ctggggattc tggatgccag cgaatgtccg cccacctacg
    20461 gcgtgaaacc gggtctggtg gttggtttga ttgctggagg cgaatatgcc attcagcacg
    20521 cggtggaagg cgctgaagat agccgggaag gtggtgttaa cgatctgaaa aatattaatt
    20581 taacggcaca ggatgtggtg gttggcattg ctgccagcgg tcgcacgccg tatgtgattg
    20641 ccggactgga atacgcacgc cagctcggct gccgcacagt gggaatttcc tgtaatccgg
    20701 ggagcgccgt ttcaaccacc gctgagtttg ccattacacc gattgtaggt gccgaagttg
    20761 ttaccggttc ttcgcggatg aaagcaggca cagcgcagaa actggtgctc aatatgcttt
    20821 ccaccgggct gatgattaaa tccggcaaag tattcggcaa cctgatggtc gatgtggtcg
    20881 ccaccaacga aaaactgcat gtgcgccagg tcaatatcgt taaaaacgcc accggatgta
    20941 atgccgaaca agcggaagcg gcgttaattg cctgcgagcg caactgtaaa acggccattg
    21001 tgatggtgct gaaaaatctc gatgccgcag aagctaaaaa acgcctggat caacacggcg
    21061 gctttattcg tcaggtttta gacaaggaat aacccatggc caaagagatc agcagtgaac
    21121 ttctgaacac cattcttacc cgtgtcggcg gaccgggaaa tatcgccagt tgtggtaact
    21181 gtatgacgcg cctgcgtctg ggtgtacatg acagttcact ggttgatccc aatatcaaaa
    21241 cgctggaagg cgtgaagggc gtcattttga ccagcgacca ggtgcaggtc gtttttgggc
    21301 ctggtaaagc gcatcgtgcc gcaaaagcga tgagcgagct gctgggcgag gcaccggtac
    21361 aggatgccgc agaaatcgcc gcccagaaca aacgtcagtt aaaagccaaa caaacctccg
    21421 gcgtgcaaca atttctcgcc aaattcgcca ccatcttcac gccgctgatc cccggtttta
    21481 ttgccgccgg tctgctgctg gggatagcga cgttaattgc cacggtgatg cacgttccgg
    21541 cagatgctca gggcacgcta cccgacgcgc tgaattttat gaaggtgttc agcaaaggtt
    21601 tgttcacctt cctagtgatt ctggtgggct ataacgccgc ccaggcattt ggcggcacgg
    21661 gcgtcaatgg cgcaattatt gccgcactct ttttgctcgg ttataacccc gctgcaacca
    21721 ccggttacta cgccggtttt cacgatttct ttggtctgcc catcgatccg cgcggcaata
    21781 ttatcggcgt gctgattgcc gcgagggcct gcgcccgcat tgaaggcatg gtgcgccgct
    21841 ttatgccgga cgatctcgac atgctgctga cctcgttaat caccctgctg atcaccgcta
    21901 cgcttgcgta cctgatcatt atgccgctgg gtggctggtt attcgaaggt atgtcatggc
    21961 tgtttatgca cctgaacagt aatccgttcg gttgtgcggt tttagccggg ctgttcctga
    22021 tcgccgtggt atttggcgtg catcagggct ttattcctgt ttacctcgcg ttaatggaca
    22081 gccagggatt caacagctta tttcccatcc tttcaatggc aggcgcgggc caggtgggcg
    22141 cggcgctggc actctactgg cgggcgcaac cgcacagtgc gctacgcagt caggtacgcg
    22201 gggcgattat tcccggcctg ctgggcgttg gtgaaccgct gatttacggt gtcaccctgc
    22261 cccgcatgaa gccgtttgtt accgcctgtt taggcggcgc ggcgggcggt ttgtttatcg
    22321 gattgatagc ctggtggggt ctgccgatgg gcttaaacag cgcctttggg ccgtctggtc
    22381 tggtggcgct gccgctgatg acttccgcac aaggcatcct tccggcaatg gcggtttatg
    22441 ctggcgggat tctggtggca tgggtttgcg ggtttatttt caccacgctc tttggctgcc
    22501 gtaacgtcaa tctggactga tattatgaaa cggacaatgc tctatctttc tctgctggct
    22561 gttagttgta gcgtcagcgc cgcaaaatac cctgttctga cagaaggtag ttgatcaagt
    22621 atatgcggcg ttaaagcagt aataaaaagg ccagtcagta atgttactgc ctggccttta
    22681 atcaatgaat cagaaacgct tacagcacca tcaacttgtc cagcgacggt gcgaaataat
    22741 agccgccggt taccggtttg gtgaaacgca acatcgcatc acgtttgcca tcggtatcgc
    22801 caaacatgct cagcagttgc tgctcaatgt tatgcagacg cgcgcagtag gcgcagaagt
    22861 acagaccgtg agtgccactg gcggtgccgt acggcaggct ctggcgaaca atcttcagcc
    22921 ctttgccatc ttctttcaga tcaacgcggg tgaggtgaga ggtttccgga cgttcgtcgc
    22981 cgtcaatctc ttcgttggcc tctttggtgc gcccgatcat catctcctga tcgtgaacgc
    23041 tcatccggtt gagctgcttc aggttgtgct cccaacgctg cacaaacaca tagctgccgc
    23101 cggcatccac gccgtctttg ataaccgcca cttcgcggcg cgtctcttca cccgccgggt
    23161 tttctgtacc gtcaacaaag ccgctcaggt cacgctcttc cacccaacgg aagccgtgga
    23221 tctcttcttt cacttcaatg cagtcaccaa aggcttccat cgccgcctga gcgacagaga
    23281 agtttacgtc gtgacgcaga gaaagaatgt ggatcaacac atcgaactgg gtcgtcggcg
    23341 caaggccttt accgtagccc ggaaaatctt tcagctcttc tgccccaacg ccgccgctca
    23401 gagcgcgcca ggtgttgtta ccaaaggcaa ccaccgcacc aagatgcgcg tccgggaatt
    23461 tcgcttcaaa agttgccagt ttgtcggcaa atgttttact ggccgcacgc agggcgtcaa
    23521 cttccccttt cacgttggct tcgatccaaa tcgccgcgcg gcaatgttct ggcaaaatgc
    23581 cactctgaac ctgagacatt gttcctcctg aaaataagaa tgccacgcaa tccgtggcat
    23641 tgttgagcgc tattttacgt gttttttcgc gacggcggtt tgttcagacg caaattaacg
    23701 ccgccagata attttactga ctttccagtt tttcagggta tcgtccgaag gcattaaccc
    23761 ttcaggacca cgccattccc cgctgaactg gtagctaata tgttgactgc cttcggcttt
    23821 acattcgacg gcacgattat catcaccatc agctttttgg caattgccaa atgctttgct
    23881 gtaaaggtcg ctaaacggtg taccgatttt aacaccggtg tcagcaggaa tatcgctatc
    23941 cagcacgtca atgcgactga tcgtgccttg atcgccgtta atcaccatcg ccacgttgtc
    24001 gcctttcatc acttcaaaaa agcgcaccac gttgccgttc gcggttttca ttccgctgcg
    24061 caggcgataa tcgccatcaa gcgcatcagc aatggcttgt tcttgcagtg gtgtggacgc
    24121 cgttaattca cccacgccct gctcgctcac tttggtggac gatccaaacc agttccacgg
    24181 gttagcggca gaccagttaa ctgacgacat cgtggaacag ccggtcagca tcaacggcat
    24241 agcgcataac attaaacgca gcgatttcat gtcacttcct ttggttattc aataacgttg
    24301 cttggagtgc aaattcacca aaaagtgccg ttattctttt tcttcattaa aacaagcgcg
    24361 taaacgtcga ttggtcagca gccagattaa cgccacaata tccgccacta ccagcgccag
    24421 accgataccg ctaacggatt caccgttcag ccacagccac ggttgccagc aaagtaaaac
    24481 cacctgcgcc aacagcaaca gaaaatagag cacatgccag gtgcgaggaa acgaagcccg
    24541 ccgaccgctc aacaaaaacg ccagcaccgc cggaataccg ggaatcagcc ccagccagaa
    24601 attatcgtga tcgggataaa acagatttag cagcgcagta ccctgctcgc gcgacgcacc
    24661 ggcaatgaca aacagcaccc aggttcgcgc ctgaagcaat agcacaagcc agaagagcaa
    24721 gggtaaacgc aggcgtccgt gcgcatcata atggacagga tggaactcag tactcttcat
    24781 cttcaatcaa acgcttaccc agactcagca cgtcggcgtg ttcatatccc aggcgttcat
    24841 acattccgag caccatgtcg ttatcttccg gcacgttgat ctgaattttc gggcagccgc
    24901 gagcaatcag ctttttctcc agccggttaa gcaacgcatt ggcaattcca cgcccacgaa
    24961 actctggatg cacgccaaga taataagcag acccgcgatg cccgtcataa ccgcccatca
    25021 ccgttccgac cacttcaccg tttacctcag cgaccaaaaa caaactgacg tcatggttca
    25081 tcttacgctc gatgtccatt tccggatcgt tccacggact cagcaagtcg caacgctccc
    25141 aaagggtgat gacctcttcg aaatcttcct ggcgaaatac gcgtatctcc atggtattcg
    25201 ttaccttttt gcgggttaaa aggctgatta tggcgtgaac ggtcgaatta gccaatatct
    25261 gacgaaaatc ggttgaaaaa gtggcataat ggggagttgt caactattga aatgaaaagt
    25321 aaaacaattc tcaacagcaa accgtcgtaa cggattacgc gatacgatat aacatctgga
    25381 actttattat tacaactcag gccgtatgag cacttttaaa ccactaaaaa cactcacttc
    25441 gcgccgccag gtgctgaaag ccggtttagc tgccctgacg ttgtcaggaa tgtcgcaagc
    25501 cattgccaaa gacgaacctt taaaaaccag caacggacac agcaagccga aagccaaaaa
    25561 atctggcggc aaacgtgtcg ttgttctcga tccaggccac ggcggaattg ataccggagc
    25621 gatcggacgc aacggttcga aagaaaaaca tgtggtgctg gcgattgcta aaaacgtccg
    25681 ttccattttg cgtaatcatg ggattgatgc gcgtttaacg cgttctggcg atacgtttat
    25741 cccactttac gatcgcgttg aaatcgccca taaacatggc gcagatctgt ttatgtcaat
    25801 tcatgccgat ggctttacca acccgaaagc tgccggtgct tcggtatttg ccctctctaa
    25861 ccgtggggca agtagcgcaa tggcgaaata cctgtctgaa cgcgaaaacc gcgccgatga
    25921 agttgccggt aaaaaggcga ctgacaagga tcacctattg cagcaagtac tgtttgatct
    25981 ggtgcaaacc gatactatta aaaacagcct gacgcttggc tcgcatattc tgaaaaagat
    26041 taagccggtg cataaactgc acagccgcaa caccgaacaa gccgcgtttg tggtgttgaa
    26101 atcgccgtcg gttccttcgg tgctggtgga aacctcgttt atcaccaacc cggaagaaga
    26161 gcggctgtta ggcacggcgg cgtttcgtca gaaaatcgcc acagcgattg ctgaaggcgt
    26221 gatcagttat ttccactggt tcgacaacca gaaagcacat tcgaaaaagc gataagttat
    26281 gaaacccgac gcacaccagg ttaaacagtt tctgctcaac cttcaggata cgatttgtca
    26341 gcagctgacc gccgtcgatg gcgcagaatt tgtcgaagat agttggcagc gcgaagctgg
    26401 cggcggcggg cgcagtcggg tgttgcgtaa tggtggtgtt ttcgaacagg cgggcgtcaa
    26461 cttttctcac gtccacggtg aggcgatgcc tgcttccgcc accgctcatc gcccggaact
    26521 tgccgggcgc agtttcgagg cgatgggcgt ttcactggta gtgcatccac ataacccgta
    26581 tgttcccacc agccacgcga atgtgcgttt ttttattgcc gaaaaaccgg gtgcagagcc
    26641 cgtttggtgg tttggcggcg gcttcgattt aacccctttc tatggttttg aagaagacgc
    26701 cattcactgg caccgcaccg cccgtgacct gtgccagcca tttggtgaag acgtttatcc
    26761 ccgttacaaa aagtggtgcg acgattactt ctacctcaaa catcgcaacg aacagcgcgg
    26821 cattggcggg ctgttctttg atgatctgaa cacgccagat ttcgaccact gttttgcctt
    26881 tatgcaggcg gtaggcaaag gctacaccga cgcttattta ccaattgtag agcgacgtaa
    26941 agcgatggcc tacggcgagc gcgagcgcaa ttttcagctc taccgtcgcg gtcgttatgt
    27001 cgagttcaat ctggtctggg atcgcggcac gctgtttggc ctgcaaactg gcgggcgcac
    27061 cgagtcgatc ctgatgtcaa tgccgccact ggtacgctgg gagtatgatt atcagccaaa
    27121 agatggcagc ccagaagcgg cgttaagtga gtttattaag gtcagggatt gggtgtaact
    27181 ccctcacccc cattcccgca tccgctgatg caacgtcaac gacggcttct cggcaaacag
    27241 ctgctggtaa tccgtggcaa attgccccag atgccagaat ccccactgca tggcggcgtc
    27301 ttttaccgtt gtgctttgcg accacgggct tatcagttcg cggcgtacgg cgttcaggcg
    27361 aatgcgtttc agccacgcgt ttgggccaat gcctaaaata gcgtgaaacg cgttttgtag
    27421 cgtgcggcga ctgacatgca gttgattaca caaatccagc accgtcaccg gttcggacat
    27481 gttttccagc acatattcac gggcgcggga aagcaaccga cggtaactct gatgactgat
    27541 gctttccgcc gtcaccattg gttgcgcttc ttccagcatc gcccccatcg ccattagcaa
    27601 attatccccc agcacttttc gcactgcagg ctgatggaga ttttccgggt tctcgcaaaa
    27661 cgtcgccagc gcctgttgga caaagcccca cagcgcggct ttatgctgct tctttcactt
    27721 ccagcgccga ctggctacgc agcatatgta atacccgatc cggattgtgc aaaaagttag
    27781 cctgacaggt gatgacatct tcagacagca ctacgcccag gatcgtataa tcgtccggcg
    27841 tacttaattc aaactcggtt ccaccggggc gcgttgctat ttctgcgctt cccagacatt
    27901 gcgaaccgat aaatccctgc tcaccgcgcg tcgccggaat gccaaaccag aacgagttcg
    27961 gccagaccag gcacgactga cgcagcgcca gaccggtgta ttcacgaaaa acctgaatat
    28021 catcgagtag aatttccgta aattcaccat gaaacttgcc cggatgcagc tgatcgtaaa
    28081 tctgctgcca ggcggtaatc gtcaaagcat gttcatagac atccgttgtc tgtcgttgat
    28141 gaacattatc cacttcgacc ttcggcgtga gcttcaggtt ttcgggtaag ggttcatgat
    28201 aaagatggtg caaattggct gtacgggtct ttttcatgat gttaatgccg ggtgttgtag
    28261 gacacccggc acctccgaca ggttaatggg gcttgagacg ataacgacta ctgcgtttac
    28321 gttacgtccc ggcagaaaag agctgttcga gcgcatttct ggctttttcc agcggccagc
    28381 caaagtgggc ggccacttct cctgccgtca ttccctgaca tactgatgtt aacagcgcca
    28441 acaactcgtc agcagattcc gcgacgataa ctggcgcgtc gggttccttt acgggttgct
    28501 tcggctggcg gttaaagcca gtaaccagcc actgggtgtc atcgtctggc cgaccgatct
    28561 ccttacggct gatgacacgc ccggtacgca tcgcggcagc acaacctgcg tccagcgccg
    28621 cacgacacgc cgccagatcg ccttccacca ccagcgttaa gcgaccaggg tcaagcactt
    28681 cgtgactgag cagacgaacg ttagctgctt taagcatggc atccgctgca tctatcgcgg
    28741 cgaccatgcc gtccacttcc agcaatccca gtgcattgat catcggcaac ctccgttacg
    28801 cacgctggat tggattacgc gcgatttcca gcactgcatc ggtaaaggcg ttacaggctg
    28861 ctttacacgc ggcctggcta ccggttaaaa atgctgccga gtagttggtt tcagacggtg
    28921 gcgggacgta ggtcaccagc tgtacgtcgg cagatttcaa cgcggcatca atgccgtagg
    28981 tcgcttccag cggcggtgcc accagatacg ccatcggatc gccaagcgtg atcccggcgg
    29041 ttgatgaaag ataagaacct gtacgcgaaa ccacatgcgc caggaaggcg gtgttttctg
    29101 cgtcattggc ccactggaaa gctgcgccat tttcaatatg tgcaaccatc gcatccagac
    29161 cggcacgcac ttccgccggg tttggcccac caagcataat cagcacttca ccggcagtcg
    29221 gtgacgggcc gtgtgccgct cccgcataca gcgagcgacc atataccact tcgaccatcg
    29281 cttgtttggt cgcttcgtca gccgcaatgt aggttacgtc gtcagaatcg gcagaaatca
    29341 gcccgaggct acgaatatgc ggcggcaatt ttagttcacg cgcaaattca gcgttaacgg
    29401 aggcaatcac ccgcatggcg gttaccgacg gtcgaatcaa atctaaagct ggcatgatgc
    29461 ctccttatcg ggtcatgttg atgccggatg ctttctgctc cagcatacgt ttcgccaaat
    29521 ccacaatgac ggctgcggct tcaaccggcg gcgtgccgcc ctggtgaatg ttagagatac
    29581 aggtgcgatc ggcctcaacg gtggtcgcca tacgcggcga gtaaacggcg tagcaggaga
    29641 ggctttctga ctgccctaac cccggacgtt cccccaccag caggatcacc actttcgcgc
    29701 cgaggatctc accgatctga tcttcaatct tcacgcgacc ataacgaaca aagaatggcg
    29761 tgccgacttt cagcccggcc tgtttcaggc ccgccatcag cggcggcagg atctcttcgt
    29821 agttgacggt gatggcatca gttgacaagc catcagaaat aacgacctgt acgtccggat
    29881 tagcagcaac acactgtgct ttcagcgctt caacagcttc tgcacacagg cggcggccca
    29941 tatccgggcg agtcaggtag agatttttgt cgctaatttc agagcgtact tccagcaagc
    30001 cctgcgcttt cacccactct tccggcactt ctttcagtac ggtatctttc gaacgagagt
    30061 gatcggccaa gaaacgcagc agcgcctgag tacgtggacg cggaccggca cgaccggtac
    30121 aaacgcgggc cacggtgctg cggcgcagtt ctgttaatac gtctgcgcga tgcgggtttt
    30181 caacaccaat ccacgctttt gcttcagcgg aacctaaatc cagcgcacag ctttccgagg
    30241 tcaccggtgc cgcacagttg gtggtggcgc actttgcttc tgacggcgcg ggggccgctt
    30301 gtcccattga cgccatcacg ctgcgtacaa tttcttcaat ctgtttttga tccatgatgt
    30361 gttatctccg cgtcatcaga agaacagtga cggatcgccc gcccgtttgg tcaggcgacc
    30421 gtttgccata atgcccatgc tttccagcca gcgttcaaac tctggtgacg ggcgcaggtt
    30481 gagtaactga cgcacagtgg cggtatcgtg gaatgcggtg gtctggtagt tgagcatgat
    30541 gtcatcaccc agcggcatcc ccatgatgta gttgcagcct gcggtggcga gcaggatcat
    30601 caggttttcg ttgaggttct ggtcagagtc agcgtggttg gtgtagcagc cgtcacagcc
    30661 catagagatg ccgctcagct tgcccataaa gtgatcttct aagccagcac ggataatctg
    30721 gcggtcgttg tagagatact ccggcccaat aaagccgacc acggtgttga cgataaacgg
    30781 atcgtaatga cgcgccagcc catagttacg tgcttccatc gtcacctggt cagcgccgaa
    30841 gttagcgcca gcggatagcg cagagccttg tccggtttcg aagtagaggc agttttcccc
    30901 ggcgatacga ttgaactctg cgcccactgc gcgcgcttcg tcgagcatcg ccagttccac
    30961 gccaaactct ttcagccctt tttcgctgcc acagatactc tggaaaatca gtccgccagg
    31021 agcgccgcga cgaatcgctt cgatctgggt ggtgacgtgc gccagtacgc agccctgagt
    31081 tgggatgttg aatttgtcga tcacgccata aatggtatcc agcacgcggc ttaagttttc
    31141 cacgtcgtca gttaccgggt taacgccgat caccgcatcg cccaccccga aggaaagccc
    31201 ttcatagatt tgcgcagcga tactttgcac gtcgtcacgg gtatcgttcg gctgcaaacg
    31261 ggcgctaaag gtgcccggaa taccgatggt ggtattggcc tttttgatta ccggcatttt
    31321 cttcgcgccg tagatcaggt ccgcgttgga gcaaatcttc gctatcgccg cgaccacttc
    31381 cgaggtcagc cctttgcggg taaaggcaat gtcgtccacg ctggtttcat cgctcagcac
    31441 atactcacgc agttcgctga tgctccagtt tttaatctgg ttgtaggccg tttcgttaac
    31501 gtcgtcctga atcagccgcg tcacgcagtc atcttcatag gcaatcaccg gattattgcg
    31561 gatgtccgct acggtcattt ccgacaacac ctgctttgcc gccacgcgct cctgtgagct
    31621 tgccgctgca acgcccgcca gcacatcccc cgaacgcagt tcgttggctt tagccagcac
    31681 ctcttttaca tccttaaact gatatacatt gccgaacaat gtggtcttta gtttcataag
    31741 tcgttccctc aggaagaagt gatgagtaac atttctcaca gcctgcaaac aattctattt
    31801 catcgttagc ccttgtcatg taaggtgatt tccttaacac gcccgttact tatcctgccg
    31861 aacgcaaccg aactcgccgg atacatgagc ggggttatcg agtcgttaga tcgaggtatc
    31921 cggttttgag caaaacacca ctcacagcaa aggccataga cgccgcacaa ccaaaggaca
    31981 agcaatacaa actcaccgct tcactgacgc ccggcctgtt tttgctggtg catcccaacg
    32041 gcagcaaata ctggcgattc cggtactgga tagacaagaa agagcggtta caggctattg
    32101 gggtatatcc gctgattagc ctcaaggaag cccgcaaacg cgccacagag agccgtttac
    32161 tgatagccca gggaattgac ccaatggatt tggcacgcaa ggagaaagcc attgatgcac
    32221 tcaacatggc ggcaggtttt aagacggtag cggaggactg gctggctacc agggtaggcg
    32281 gctggagtga gagctataca aagcaggtca aatcagcact ggagaaagac gtttatccgg
    32341 tacttggcaa gcgttcaatc gttgatataa ccgcccgtga cgtgctggca ttactccaga
    32401 agaaagaacg taccgcaccg gagcaggccc gcaagctacg ccagcgcatc ggtgagatct
    32461 tcaagtttgc cgttatcacc gaactggtaa cgaggaatcc ggttgcagat cttgatacgg
    32521 cactgaaggc ccgccgacct ggtcacaacg cgtggatacc gattagtgaa attccggcct
    32581 tctacaaagc ccttgagagg gccgggagcg tccagattca ggcggcaata cgtttgctta
    32641 tattaacggc attaaggacc gcagaactgc gtttgtgccg ttgggaatgg ataaacctgg
    32701 aggacgcaac aatcacattg cccgctgaag tcatgaaggc ccgccgcccg catgtggtcc
    32761 cgctatcacg gcaggcggtc gagctattgc aggaccagtt cacacgcagc ggatacagtg
    32821 ttttcgtgtt cccgggccga ttcatggata agccattatc agccagcgcg atactcaagg
    32881 cgcttgagcg tatcggatac aagtcaatag ccacaggcca cggctggcgc acaacgttca
    32941 gcaccgcact taacgaaagc ggcaggtata accctgattg gattgagatc caactagccc
    33001 acgtcccgaa gggaattagg ggagtttaca accaagcggc ctatctcaaa atgcgccgtc
    33061 aaatgcttca ggactacgct cattgccgct cggctaggtc aaatcctcac gataaagctg
    33121 ttcggattgt gaccataatg ttaaaggact ctttaaatca ttccttgacg aattaactag
    33181 ctggcttaag cggaatagca tgcaggataa aaacaaaacc agcttagcag tggggagagc
    33241 ttcgcttcgc tcgtctcctg gggccaatgc tcaatgaaac gacgtaaggc acggagcgct
    33301 caccataggt agacacataa acatctgaga gcgcaccttc tatcgcgcta cgcgctcaat
    33361 aaaaaccaac gacaccgcca cactgcaaga ccaatggttt ttatcgcgta tacagaccag
    33421 acaggaaaca gcagcaaagc agcgtatagc caggtgcaga cagcacactc acacgacgca
    33481 cagcaggctt aagtgatcac tcaggaaact ttatccccgc cgccgatcac cgcgaactat
    33541 catgaccgcg ccaaattcga aaggaatggc ccatagaacg cgatgaacac acataagcgc
    33601 taacttgctt acctgaaaac ttaacgtcgc cacagagcga ttgagagcgc ttaaaatcga
    33661 tgcgatttca ttaaattttt ctcactcgcc tgaatggtgg cgatcaggac tgatcgcaac
    33721 attgccccgc ctcgatattg agcgcataat atacacatac attaagcgga aaagagataa
    33781 gtttattgtg aaaatcaggg ggttacacat catgcgcata tcagaacaac aacggcagat
    33841 cctcgactat ctggcaaaaa acgaatcaat caccgcacga caagccgcag atctcgttta
    33901 tggtggcaat gttacccgcg tccaggttga cgccgcccgc cgctcactca tgacgatggt
    33961 tagtaatggg ctaatcagga aacaaggccg cgctttcgta gctgaaagac atatcaatca
    34021 cgcttatagc caggacgttg aagaattgcg ccgccagatc ctttgggtat tcagcaaaca
    34081 gggtcaggag tatttccttt ctgaacaccc aaatttcgcc gccaaagagt taacagtaag
    34141 cggattgtcg tgcgcactgg attatcttgt cggtgaaggt tgcgttactt acaagcagct
    34201 atggcacgcc ctgaatacgc ttgtagaaga tggtttactc attgccacgc gtgagaacat
    34261 cgaatactgg ataatgccgc gttcaggtcg ccgcaatcat gcacggtata tttcaggcaa
    34321 cagatacaga acgccggact ttaatccgac acaggaagat cgggaccgtg ccgaaatgat
    34381 gtgcagggaa taggagaaaa gaattatgga taagttattt tcgatgcagt gaacacaagc
    34441 gggcgcaatg cccgttttta ttgccttatt ccacgcctga cgaatcaatt tgatcacacc
    34501 tgagcaaatg catagccaga agttaaggcc gttatattgc gattgggagc gttttaaacg
    34561 gtatcaattt aattaattct gatgtggtga tttgcaggca aaaaaaaccc gccggagaag
    34621 cgggaaatag agacatatcg ttgtagttat taacagatca ctttgaggtc gagggcggct
    34681 aaccctcgct aatattatcc cgccattacc ttgcgggtgc aaataatctt ttattgcttt
    34741 gatgatttaa taaatagcac tttttgctaa caaaaaccgc tgtcaatata tggccaaaaa
    34801 tgtcaacaga aaaaataagg tttataatat taagtatcac atgggtaatg ggttcaggga
    34861 atcggaatcc accgcctcac ggaaattcaa aatttgcttc ttctttcctc cgtggggcgg
    34921 cttctttttc gtatcgaggt atttatgaaa gcaataacat tagaacagtt cgcagcaatg
    34981 gcgaatgtat cgaccagaac gctgaggcgc tggctaaaaa aaccatcaac cggagtccct
    35041 ttgccattta ccccacgcgg tactcgccaa ttattctggg agcacgaatg caaagaatgg
    35101 ttgtacagaa cagaacgata ataatcacgc atcacatttt gaggtaaaca tcatgaacaa
    35161 tatcgaaaga ttagaacgct gtgccgatat tatgcggcgc agatggattt atgaccctga
    35221 agcgggttta ttgtttagcc gtgaaacaaa agacgttatc aaaggcagca tgaccaataa
    35281 agggcatttg gttgtaacgg tacatgaagg taaattcaaa gtgacactca cctatcagaa
    35341 agcctgctat gtatacacat acggcgctta tgacgagaca ttatatgaag tgcaccacgt
    35401 taatttggac aaacaggata accgcatcaa gaatattcgc ctgatgccga aagaaaagta
    35461 ccgccggata cataaaaacg tccgcaaact tattcggctg ggccacacac aatggcttca
    35521 accgcaatta attgaacaat cactataaac acagtagtaa ataccatgca ttcattagac
    35581 agttattttc agcgcacgac agcccctaaa tcagccgccc aggaacgccg tgaggaattc
    35641 catgagaaag tgatgcgttc tgctgattat attgcggaca agttcgtgga aacggttcgt
    35701 ccacttgttg atgaagtagc tgataaactt cagtctgaaa tgccggaaga tatggaaggc
    35761 actgcaaagc ggcgactcat ttgcgaatta agtcgtcgtt ttggcgtgtc aataagcgct
    35821 tttaaataat cgaaaaaagg ggggaaagat aatggacgca tttgaggtac tggcaaataa
    35881 aggcgctgaa ctggtagcac agcgtgataa aaccgccaac gaaggggaac gcgcactgct
    35941 aaataaacag attaaagcga tccgtatggc ccagtttaaa ttaatcagta atgaagttat
    36001 cgaaacaatt aaaccgcaga tcccacaaat cgttgctgac gctattaaag ccgccggact
    36061 ggagaaacgc attgccgggc taaaaacgaa cgcagagaaa atggagatgc acaaacagag
    36121 cggtgctccg aatcccgatg agtacttctc accggatgtt gagtttatgg cgcaggttga
    36181 ggaacgtctg ggtagctgtc tcaccgagga acagcgtcgc tattttgatg gagtggatag
    36241 ttcggcaggt attgacctga actcgtattt cggtcgtgaa attgagcatt ttgatgccgc
    36301 cccgtctatg ccggagccag aaccagaaat ggccaccgat cctgaagctg agcaacggaa
    36361 acgcgtattc aatgcgatag ggattcggta ctgatcggat aaaagaaagc cgggaattac
    36421 cccggctgac ttaacaatgt gaccaatgaa caaatcggac aagacaaaca ggataagcag
    36481 tctcgaaaca atgttcggga actatattag cacaagtccg gagaaaatca tcatgttggc
    36541 actacgcatc atgcagggca tcgctaagac cctcgcagaa catgtcttag acctgaaaca
    36601 ttcgccactg agcaaacagg ccatgaagcg ctaaacgctt cgtctgtggg ctgaatattc
    36661 gcttggaact atcaataaaa tcatcgacat gaaatcgggg ccgagcaacc agagcgcgga
    36721 ggaaatggag ttcatcaggc gtttaattct cattcgcaga gacattcata gccagttaca
    36781 cagcgtggga atcgatatta atgatgggac gggcgattaa ttttcggaca agtactatgc
    36841 gcgcgcgagg agctgaacat gaagcaagat attgcagaca ggctggagat actcgaaggc
    36901 cagcgggcag aagcgaaaca actacgtaag caggccagaa gagcgcacag gaacaacgaa
    36961 gcagagttac taaccaaata catcagtttc accaattact gcatctacga gtgttacaag
    37021 gaagatgcag aggactggct ggattcattg ccagaacagt actgagcaga ataacaccac
    37081 gtcatgttgt tctgaggtga gcatatcacc tctgttcagg tggccaaatt tagtgtgcca
    37141 cttcagcatc accgttgaaa tcacaataac gatttttttg taaatatgct ctcacatatg
    37201 taggaataga ccaggttggc attgaaacac atctaccatg accataataa agtaattttt
    37261 tgatatgttt atggttttat attaattaaa atatatctaa aattgatagt tttaaaattg
    37321 caaaatccag attgtgtttg taatgtttaa tctgattgta ttgagtggcg tttattatga
    37381 aaaataaatc aggtaattct tgttatagag aagcagtcga tattcatagc tcacttgagc
    37441 tttcatttat attgcataaa tatcatttgc gtgtagatga gaagaaatct cgaaatgtat
    37501 tcagcgccat acgtgatgag ctcatccttg atggggattc acgacaaaac ttagctactt
    37561 tctgtcaaac atggcttgat gatgaaatcc gtgagttgct taatttagct attgataaaa
    37621 acatgattta taaagatgaa tatccacaaa tggcggaacc tgaaggtcgc tgtgtgcgta
    37681 tgctttccga ttcgtggagt ttccccgatt cgcgacacac cttaggttgc tcaaccattg
    37741 gttcatctga agctgctatg ctcggccgct tggcgttaaa atggcagtgg tgtaaaaaac
    37801 gcgaagtaca aggtaaatca accgaacctg atttgcgatc ctgtgcaaat atgctggcat
    37861 aaattctgcc gttactgtga tgtgaaattg tgtgaaattc cacttgatga tcatcacctg
    37921 ataatgaatg ctaaagaggt tttgaagcat gtcgatgaaa acactattgg tgtcgttacg
    37981 actttaggta ttacttattt ttgtaaagca gattgcgtta gtgtttttaa gataagtgtt
    38041 aaagttaata ttagttttta tatttacatc gtaattatat gtttctattt taaaaatatt
    38101 cgagagtata aaaatgtcta atgctaatat cgcacccaaa agtaagaaaa tgacagtggg
    38161 gacactcgct atcatgaata taactgctgt agtaagtcta cgatttatac cttctgaagc
    38221 agaatacggc ttaggtgcca ttttttacta tacctttgct gcaattatgt ttttagtacc
    38281 tgttgccttt gttgcggctg agctagctac aacatatcca caaaaagggg gggcatttcg
    38341 atggatagga gaagcatttg gggctcgctg gggttttgta ggaatgctta tgacctggct
    38401 acaagtcatc ccctactttc caacagtact gacatttggt gcagtctcgg ttgcatttat
    38461 tgatgttgat atgggggtgg cagaatcaat tgccagtaat aaatggtata ttttattttt
    38521 cgtactattt gtttactggg gagctatttg tgttgcatta cgtggcgttg gtattttctc
    38581 gcaggtatct aagtggtgcg gtattatagg gacaattatt ccagctatta tccttgttat
    38641 tcttggtttc tcctatctgt ggttcagcgg taaaccagcg gtgatcccat taggttgggg
    38701 tgatcttttc cctaatatag ctaacttcca gaatattgtt cttgccgcca gcatatttct
    38761 tgcttatgca gggatggaaa tgaatgcggt ccatgttaac gatttagata atccaactaa
    38821 aaaataccca attgcaatta ccatcgcttc tataggtact attgcaatat tcctgctcag
    38881 tactttaggg gtcgccttta ttattcctac agataaaatt agtctaactc aaagtttgtt
    38941 ggttgcctac gatatgctgt tcgaatgggc tggagtgcca tggcttggct ctgtgatggc
    39001 atttatgcta gcaattggcg tactgggtgg ggtggttact tggattgcag ggcctaatac
    39061 aggtgtatta gcgatcgcga aagctggtta tttacctaag tttttccaaa aaaccaatcg
    39121 tcatggaatg gggcaccatc taatgtttgt acaaggtata attgtctcag ttctttctgt
    39181 tacttttgtg ataatgcctt cagttcaagc agcttttcag attttatcac agttaacagt
    39241 tatgctctat ttagttatgt atatgctcat gttcgctagt gctatctatc tacgacattc
    39301 tcaacctgat acaaaacgcc catatcgagc ccctgcttta tttttctggg caacacttgg
    39361 attcttaggg tcttttctgg cttttgtatt aagttttatt ccgcccgagc aaatcccaac
    39421 tggtagtcca accagctatg ttctttattt gttagtatta gttgccctat ttttattaat
    39481 tccaatatta atttacgcct tccgaaaatc tgactggaaa gatccaaact ctgatttcga
    39541 accattttct tgggaaaaaa gaactagtaa ttaaattttc cataagctac aaaagatgag
    39601 gttaaatatg tttagtgctg aatctccaat tggtgtaata gactcaggtg taggtggatt
    39661 gacatcggta aaggaaataa ttaatttgct tcctggtgaa gatattatct attgtggaga
    39721 taatatgaat gcaccttatg gcaaccgttc tgccgatgat atcatcagtt tgactaaaaa
    39781 aatgctgact tttttacagt caaggaatgt caaactagtt gctgtcgcat gtaatactat
    39841 atcctcaact ttggaatccg aaaaatacag cggatatgct aagagctttc cgtttcctat
    39901 cttatccatc attgaacctg cagtagaaga tgtgattcgc cagcaatata aaaatgttgg
    39961 tattatcgct accgagttta ctattaaaac ggggtgtcac aaagagctaa taaaaaaatt
    40021 gaattcaaca ataaatgtat ttggagaacc aagcaaaaat cttgcaatgt taattgagga
    40081 aggtaatctt aatgctcctg caatcttgaa tgacatcaaa aaacatgtta accatttaat
    40141 ttccctccat ccagtaaatg aaattattct cggctgtact cactatccaa ttgttcagaa
    40201 tttttttgaa gcagttgcac cggatataaa atttattaat cctgcacatg atcaggctat
    40261 ctctattaaa aatcacttgg gacagctaaa tttattaaac tcctcaaata taggaacttt
    40321 gcaaataaat actagtggaa gtatggagat ctacaaaacg gtactagggg aattgagcat
    40381 tacgaaacct catacattca gtatcaggca gttttaaatt tatccggtgc accgtaaaat
    40441 cccgtccttc tgaactgcac cccaaaagtt ggacaccaac tgagtaaggt gcattttatg
    40501 gacaaaccca gttacatact gggtagtgca tccaattagt agaacatgtg tttttcgata
    40561 aacgctccga tcactttttc gtggatctca actgagcgtg agaagcagat tgttttacga
    40621 cccaaccgct taatgcgggt gcgtagcgtc agattattac gctcaatgcg ttgggtgaat
    40681 attttgccgg tcagatgctt attcttcggc acctcccggc catagctgcc ccagtcatcg
    40741 ctggtcagca tgccgatgtt gaagggtgta agcagtgcca gtagctcccg gcacgtttga
    40801 tcggttcggg gaccaaaagt gtaggccagt acaccgcctg ttttggtgtt gtacgcgtac
    40861 ccgagccaat gttgccgggc tttactgcca acgtagctcc attgctcatc aagctcgcag
    40921 ataaacgcca catcagcatg ggcaacaggc gaagaagtta ttcgctttgg cgcgagtttt
    40981 ttaaagtccg gatgacggtg ttactaccaa ttttcagtgt cctggcggta tcgcgaaccc
    41041 cggcaccatt aaaggccatt tcagttatca gctctttcat acccggctta cgtgcttgat
    41101 aagtataagt gaactgaaac acacggtggc agtcacggca gcgaagtctg tcacggcctt
    41161 tagggttctg accatggcgg taaacctgag ctgactgaca acggggacaa tgaatattaa
    41221 cgctggtcat gagataacct caaaagcccg tattatacat cagattcaac taattagagg
    41281 catcacccat ccagcattat aaaacatact aacaataact aaattataat aatcacaact
    41341 tacaatcaaa taataaatgc ataagattaa caatgatttt ttcgggttat atttatctaa
    41401 aaaagcagaa tattatgtaa cttattattt tctaagcgaa aatttattgt gcggtcagac
    41461 taacaaactt gcgtaccacc cgaagaacat gcgaagattt aagtattctt tcttttttgg
    41521 tttttatttt tcattttctc ttttcttctc tttccggttc tcttctgttc tggttttggt
    41581 tttttccttt ttatttttat tctttcttca gggttatgct aaaacaccaa aacatttttc
    41641 accacaaaag catccaatca tcttgccaga ccaatcacct taaccggata cactgccgga
    41701 tataatccca ctaacaccca gccaccaagc accatacctg ataactaccg tattcaggaa
    41761 ggaaatgcga gtgatttcac cgtcaccggc acaaccgatc cgccaaaaag aggcgtacca
    41821 atgtcgatat agtcccccgc gcggacaatc acttcgtcaa tgactgccaa cgggagttgt
    41881 tgtagctgtg ggcgcaacaa catgcccaga gctttaccaa agtcctgccc ggccaccacc
    41941 agcaggggat gcggattcgg aaaacgcgcg acgaaatcga ccagcgcgtt gatgaccgtc
    42001 agtaccgcgg cgtaacgcac aggcagcgag gcgggaagcg ccagcacgta cacgtcagtt
    42061 ttgggatcaa gatccagctg aagcagcgcc tgttgccagg cactcaccag atccgtttca
    42121 tcaatcggga tcgccaccgg caaattgcgc agcggcagtt gtacgccctc cagccagatt
    42181 gtgctgccag agagcgaaag ggtatgtgca cccgcgccaa tcaccgtggc gcatacggtt
    42241 tgcgccggaa actgcacatt catctcacgc aggcgcggat ggtcatgcag cgccgttgcc
    42301 agaagtgggc caatatcggc aaaacagaac gggtcggcgg gctggtggcg ataacattcg
    42361 cccacgcctc cagaaagcgt aatgatttcg ggcgtaacac ctgcgggcag caaaccggtt
    42421 tgcatcaatg cctgcgcgag cggcgaaagc gttccgtcaa tcacgtcgac aatcagcgcc
    42481 gccatccgcc gggtaacctg caccagctgc gcgccggtca gcgaacgggc gtcagtgcct
    42541 gcaccgaagc actcatccac aatcatctgc cccggtttat gagcgtaaac cacgcgcccc
    42601 tggctgtcgg tttccagcag gcgaccaccg acgttgagac aggcagtgcc gctgattttt
    42661 ccggcatcga acagggcgta gttcgcggtg ccaccgccga tatcgatatt cagtacccga
    42721 cacagccgtt gttcagaaag ggtttgcgcc ccggctccgt gaccggcgat cacggattcg
    42781 aggtgcggcc cggcgctggc gacgacaaaa tcgcccagcg actgtttttc cttcatcact
    42841 ttttccatca gctgtgcgac caggctttcg gtaaactggc cttccggcag ctgggcaatg
    42901 atggtttcgc ggatgcgctg gctttcggtt ttgtcggctg gcacgctggc gggcacggac
    42961 gccgttaccg ggatcgattc atcacattcg gtgatggtaa aacccagtag atccgacact
    43021 tcacgggctt ccggggtaat aatgctggcg cgcagaacca cggacattgc ctgttcgccg
    43081 cgcgcgtgtg cttcacgaat atcattcgct gtgatgagtt ttttcacctg cttcctcctg
    43141 tttggtgaga gatgccagat aatccaccag ttgctgcacg ctttgcgggt cgtggctgtt
    43201 tagctcgaaa atcggctctt cgaaacccgt ctcaagcaat aactttcgcg ttgcggcgac
    43261 gtcagcatct ggcatgtccg ttttactgat gacgacgatt tgccgtttgc tgacgccaat
    43321 atccagcaac ccggcaggca accgactttc cgggtcattt gcgccatgga catagatcag
    43381 catgtctaca tcctgtagcg tggtaattaa ggcgtgatac cagcggggat ggctaaaata
    43441 ttcacccggc gtatcaatat cgcccttatc attaaattcc acggcctgtg tttttctggc
    43501 gagggtataa ttcccctgca acgcattaaa taacgttgtt tttcctgccc cgacgctacc
    43561 cacaaacgca attcgtttca tgaatgcctc gtattaactt ttggtcattt cacaaagcgt
    43621 ataatttaat aagcgaccta aaccgctgac ggtttgtgat aacgcttctt ctactgcgcc
    43681 caccgagcca taaatcacca atgcaccgct gaagcggtcg agaaaaccga tatgcacatc
    43741 agcggctttc agcgctaaat caccggcgat catcgccgtt tcgccgggag ttagcgtcat
    43801 aatgccaatt gcgcccgcat ccggaacgcc gattttcttc gccagttcct caccgggatg
    43861 cgcgatgaga tgcgccagtg tgacctgttt gccgggcaca aattcctgaa tgatgcgttc
    43921 tttatccatg agtcgccgcc tgtgaaaaac tttcgtgtat cgtgacaatt tttcgtcaga
    43981 gtaaataaca aaattcggca agcgcgttta aaaggtgagg tagatcacta agaatggaga
    44041 gatatttact gttatgaata aactttcata tgcgacgcat gaatataaat aaaataatat
    44101 cgagatattt tatatactga tttttttatt attcgcaaac tcagtaataa gctttgctaa
    44161 tatatttcag gaaaaccgct taccctgaat attcagggta agcgtgagag ttaagaatga
    44221 attacagcgg ttgggtttgc gcttcaacca cggccagcgc caccatattg acgatacgac
    44281 gcaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc cacgcccatc agcaccggac
    44341 cgacagtcac accttccgag ctggaaacgc gcagtaagtt gtaactaatg cgggcagctt
    44401 ccatgttcgg catcaccaga atattggcag aacctttcaa agggctgtcc ggcatacggt
    44461 cgttgcgaat cgcttccacc agcgctgcat cgccgtgcat ttcaccatca atcatcagat
    44521 ctggcgcacg atccctgacc agttccagcg cctgacgcat tttgctcgac gacgggcagt
    44581 cagaagaacc aaagttggag tgcgacaaca gagcaacgcg cggctcaata ccaaaacgac
    44641 ggacagtttc tgccgccatc aaggtgatct ccgccagctc ttctgcgtct ggttcgtcat
    44701 tgacgtaggt atcggcaaca aaggtgttac cactcggcag cagcagcgcg ttcattgcac
    44761 ctgcggtgtg aacgccatcg cgataaccaa agacattttt caccacgcta aaatgttcat
    44821 gataatcgcc caccgtaccg cagatcattg catcggcttc cccacgctga accatgatcg
    44881 cgccgatcac tgtcgggtta ctgatcagcg cacgctgcgc ctgttcctga gtgacaccgc
    44941 gacgcttcat gatctggaag tattcggtcc agtactcttt aaagcgcgga tcggattcgt
    45001 tattgacgat ttcaaaatca acgcccgctt tgatctgcaa gcccagtttc tgaatgcgca
    45061 tttcgatcac gttcggacga ccgataagga tcggtttcgc cagtcccagc gttaccagtt
    45121 cctgagtcgc atgcagaacg cgcgcctctt ccccttccgg cagaactacg cgcttcggcg
    45181 ctttgcgagc ctgggagaaa atcggcttca taaacaggtt ggttttgtaa acgaactcag
    45241 tcagcttgtc gatgtagacg tcgaaatcag caatcggacg cgtcgccacg cccgactcca
    45301 tcgccgcttt tgcgaccgca ggagcgatct taacgatcaa gcgcggatca aacggttttg
    45361 gaatgatgta ttccggacca aagctcagat cctgaacgcc atacgctgaa gccaccactt
    45421 cactctgttc tgcatgggcc agttctgcaa tcgcgcgtac cgccgccagt ttcatctctt
    45481 cgttgatggc ggttgcccca acgtcaagcg cgccacggaa gatgaacggg aagcaaagaa
    45541 cgttgttcac ctggttcggg tagtcggaac ggccggtaca gatgatggca tccggacgca
    45601 cttctttcgc cagcggcggc agaatttccg gttccgggtt cgccagcgcc aggatcattg
    45661 gtgcccgagc cattttcttc accatttcct gggtcagcac tttcgggccg gaacagccca
    45721 ggaaaatatc cgcaccttca atcacatcat cgagggtacg tttgccgtca tccaccaccg
    45781 catatgcggc tttggtttcc gccatatttg gctcacgccc ctgatagata acgccttttg
    45841 aatcgcaaac cacgatgtta tgtttttgca gccccagcgc taccagcagg ttcatacagg
    45901 cgattgctgc ggcacccgcg ccggaaacca ccatccgcac gtcggagatg tttttctcca
    45961 ccacgcgcaa gccgttgagg atggcggcag tgctgatgat tgccgtgccg tgctgatcgt
    46021 cgtggaatac cggaatattc atccgctcgc gcagtttctg ttcaatatag aaacattctg
    46081 gcgctttaat gtcttcgagg ttgatgccgc cgaaggtcgg ttcgagcgcg gcgacgactt
    46141 caataaattt gtccgggtcg agttcatcaa cttcaatgtc aaatacatca atcccagcga
    46201 atttcttaaa tagaacgccc ttgccttcca tcaccggttt gcctgccagc gcgccaatgt
    46261 tgcctaaccc cagcaccgcc gtaccgttag agatcaccgc aaccaggtta ccgcgcgcgg
    46321 tatatttgta ggcttttagc gggtcttttt cgatttcaag acaaggtgcg gcaacgcctg
    46381 gtgagtaggc cagcgccaga tcgcgctgcg ttgccagagg cttggttgga gaaacctgga
    46441 ttttccctgg aactggaaat tcatggaaat caagtgcact ttgttttaac tggtcatcca
    46501 tttggttgtt cctttcacgt aacgttcaca aataaagtgt gtgggcaaca gcccctgccc
    46561 acaacgtggc gcacattatt accctgccgg agtctacaga ctttgagcaa gtccaaactc
    46621 tcaccattaa tataatgttt tggtaacaat cctataacac tgatgttacc tgcttaatcc
    46681 agcaatacca tgcctgtctg ctatgctttt ttgatgcgtt tagcgaaatt tctcagaagt
    46741 gtgaattaac gcactcatct aacactttac ttttcaagga gtatttccta tgaacgagtt
    46801 agacggcatc aaacagttca ccactgtcgt ggcagacagc ggcgatattg agtccattcg
    46861 ccattatcat cccctggatg ccaccaccaa tccttcgctg ttactcaagg ctgccggatt
    46921 atcacaatat gagcatttaa tagacgatgc tatcgcctgg ggtaaaaaaa atggcaagac
    46981 ccaggaacaa caggtggtcg cagcgtgtga caaactggcg gtcaatttcg gtgctgaaat
    47041 tctcaaaatc gtacccggtt gcgtgtcaac agaagttgat gcacgcctct cttttgataa
    47101 agaaaagagt attgagaagg cgcgccatct ggtggacttg tatcagcaac aaggcgttga
    47161 gaaatcacgc attttgatca aactggcttc gacctgggaa ggaattcgcg cggccgaaga
    47221 gctggaaaaa gaaggtatta actgcaacct gacgctgctg ttttcttttg cccaggcgcg
    47281 ggcctgtgcg gaagcaggcg tttttctgat ttcgccgttt gtagggcgta tttatgactg
    47341 gtatcaggca cgcaagccga tggacccgta tgtggtggaa gaagatccgg gcgttaaatc
    47401 ggtgcgcaat atctacgact actataagca acaccactat gaaaccattg tgatgggcgc
    47461 gagcttccgt cgcaccgaac aaatcctcgc cttaaccggc tgcgatcgac tgactatcgc
    47521 accgaatttg ctgaaggagc tgcaggaaaa agtttctcca gtggtacgta aattaatccc
    47581 gccttctcag acgttcccac gcccagcacc catgagcgaa gcggagttcc gttgggagca
    47641 caatcaggat gcgatggcgg tagaaaaact gtctgaaggc attcgtctgt tcgctgttga
    47701 tcaacgcaaa ctggaagatc ttcttgccgc caaactataa accagccacg gagtgttata
    47761 tgtcccgaaa agaccttgcc aatgcgattc gcgcactcag tatggatgcg gtacaaaaag
    47821 ccaattctgg tcatcccggc gcgccgatgg gcatggctga tattgccgaa gtgctgtgga
    47881 acgattttct taaacataat cctaccgacc caacctggta tgatcgcgac cgctttattc
    47941 tttccaacgg tcacgcgtcg atgctgctct acagtttgct acatctgacc ggttacgacc
    48001 tgccgctgga agaactgaag aacttccgtc agttgcattc gaaaacccca ggccacccgg
    48061 agattggcta tacgccaggc gttgaaacca ccaccggccc gcttggacaa ggtttggcga
    48121 acgccgtcgg gctggcgata gcagagcgta cactggcggc gcagtttaac cagccagacc
    48181 atgagatcgt cgatcacttc acctatgtgt ttatgggcga cggctgcctg atggaaggta
    48241 tttcccacga agtctgttcg ctggcaggca cgctgggact aggcaagctg attggttttt
    48301 acgatcacaa cggtatttcc atcgacggtg aaacagaagg ctggtttacc gacgatacgg
    48361 caaaacgttt tgaagcctat cactggcatg tgatccatga aatcgacggt cacgatccgc
    48421 aggcggtgaa ggaagcgatc cttgaagcgc aaagcgtgaa agataagccg tcgctgatta
    48481 tctgccgtac ggtgattggc tttggttcgc cgaataaagc aggtaaggaa gaggcgcacg
    48541 gcgcaccact gggggaagaa gaagtggcgc tggcacggca aaaactgggc tggcaccatc
    48601 cgccatttga gatccctaaa gagatttatc acgcctggga tgcccgtgaa aaaggcgaaa
    48661 aagcgcagca gagctggaat gagaagtttg ccgcctataa aaaggctcat ccgcaactgg
    48721 cagaagagtt tacccgacgg atgagcggtg gtttaccgaa ggactgggag aaaacgactc
    48781 agaaatatat caatgagtta caggcaaatc cggcgaaaat cgctacccgt aaggcttcgc
    48841 aaaatacgct taacgcttac gggccgatgc tgcctgagtt gctcggcggt tcggcggatc
    48901 tggctcccag caacctgacc atctggaaag gttctgtttc gctgaaggaa gatccggcgg
    48961 gcaactacat tcactacggg gtgcgtgaat ttggcatgac cgctatcgcc aacggcatcg
    49021 cgcaccacgg cggctttgtg ccgtataccg cgacgtgcct gatgtttgtt gaatacgccc
    49081 gtaacgccgc gcggatggcg gcactgatga aagcgcggca gattatggtt tatacccacg
    49141 actcaattgg cctgggcgaa gatggtccga cgcaccaggc tgttgagcaa ctggccagcc
    49201 tgcgcttaac gccaaatttc agcacctggc gaccgtgcga tcaggtggaa gcggcggtgg
    49261 gctggaagct ggcggttgag cgccacaacg gaccgacggc actgatcctc tcaaggcaga
    49321 atctggccca ggtggaacgt acgccggatc aggttaaaga gattgctcgt ggcggttatg
    49381 tgctgaaaga cagcggcggt aagccagata ttattctgat tgccaccggt tcagagatgg
    49441 aaattaccct gcaagcggca gagaaattag caggagaagg tcgcaatgta cgcgtagttt
    49501 ccctgccctc gaccgatatt ttcgacgccc aggatgagga atatcgggag tcggtgttgc
    49561 cttctaacgt tgcggctcgc gtggcggtgg aagcaggtat tgccgattac tggtacaagt
    49621 atgttggtct gaaaggggca attgtcggga tgacgggtta cggggaatct gctccggcgg
    49681 ataagctgtt cccgttcttt ggctttaccg ccgagaatat tgtggcaaaa gcgcataagg
    49741 tgctgggagt gaaaggtgcc tgatggtgat cgccggatgc tgtatgttcc cctcacccta
    49801 accctctccc cagaagggcg aggggacggt ccgtgcgctt tttagggccg catctgcgat
    49861 tttgtttaac gggtgatcca caacgtgggc ctggcgtctg gcccatgcca gttatcgcag
    49921 gtaggttctg cggcgtaacg caccaggcga aaacgctgac cgtcaaagcg ccagcgcgcc
    49981 tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg tcaccagttc acgcgatttc
    50041 tcatcaaatg ttgcgttcat cagttccagt tcattcgtct cctgaccgtt gttgaacggc
    50101 aaacgcaacc gaaccgggcg cgaggctagc ggctttttac gcgacacaat ccatgccaaa
    50161 tcaatggtgt tataggcccc tgcctcacag ctaatcatca tcagtgcttt atcatcggtc
    50221 agcgcagtga cattcacctc acgacgtaac ggatcaagcg agcagcgcag agcattcatc
    50281 cgccagtttc cataatccag caaatcgtta cgttcttcga gtgagagtgg cgtcggcgtt
    50341 gggttaacca ccgcgacctc tttcagcgca ggcgcgggcg gtacgctgag aggcggttcg
    50401 tcccctttct tgatccacgc ggtttcactg ccaacgcgct tttgctgagc atcaataaac
    50461 aacaacgccg cttttaagcc gctcagagaa atggtctgat tgccgtcgcg taaggtgatc
    50521 gccttccctt cctgaattat ctgcaaaaac gcggtgatgg ttgccgtatc atcggttact
    50581 aataaccatg gtgaaatccg ccacttgtca ccacttaatg ccaacggctc accatctaat
    50641 agcagccgtg gcgctatctc cccttcagac gcatccggcg acttcaaccc accgcgctca
    50701 atacgtaaaa cggcatcggt atgcgcccca gcgctgcggc tcagggtcat caccagtcca
    50761 ttatgatcgc ccgtattgcg cgccacacaa aaattttgat tattacaggt gacctgccag
    50821 tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag acgtcggcaa caaagcgaaa
    50881 aaaagagaag gaaaatgcga tagcgcatgg acggtacgac cccagaagtt caaacaaaca
    50941 gcacaagtcg tatcttcgtt gcgcaggtag cgcggctcaa tcggatttgt cggattgagc
    51001 caataataaa caacgaaata ttattgctat ttttcagtcc attaaatgtg acatttgcaa
    51061 atagttaagc aataacaccg tcttaccgtc acgtatctcg ccggttttga tcatctccag
    51121 cgcctggctg aacggcagct cgagcacttc aatatcttca tcttcaacac cgccaccggc
    51181 gttagcgcgc tgattgtcac tgtattcggc gataaaaaag tggattagct cagtcacacc
    51241 gccaggcgac atatacagtt caaataattt gcgcacttcg cccacttcat agcccgtctc
    51301 ttcaatcgct tctttacgaa tgcacacttc cggttcgtcg ttatccagca gcccggcgca
    51361 ggtttcaatc agctgcccgc tttcattgcc attaacccag gtagcgacac ggaactgacg
    51421 aatcagaacc acgctctttt tcttcgcgtt gtacaggagg atcgtcgcac cattaccgcg
    51481 atcgtaaact tcacgtttat ggcggataac ttcaccatct ttgcgcgtga gatcgtaagt
    51541 aatgttgtgc agggtgaaat agttatcgga gagaattttg tctttaatga gggtgatttg
    51601 ttgcgtcata ccgactccac agcgcgaaat gaacaattat attacgctgt gaagtcgggt
    51661 ttgtctgcgc aggctatcag tgagatttga ctgattttac ccccagccag tcaataatcc
    51721 cctgtgccgc atgacgtcct tctgccattg cggtaaccac cagatccgca ccacgcacgg
    51781 cgtcgccacc ggcgaagatt ttcggattgg tggtctggta acggtactgg ctttccacat
    51841 ccgcgatgat gcggccccat ttgtctaccg ttacaccgtg cgactccagc cacggcatcc
    51901 cgtgtggatt gaagccaaac gccataatca ccgcatcggc tggcatgaca aattcactgc
    51961 cctcaatcgg tacaggtcgc cgacgcccct gggcatccgg ctcgccaaga cgcgtgcgca
    52021 ggaaacgaat tccgcaaacg tgaccttgct cattcagctc aagcgccacc ggctggacgt
    52081 taaattcgaa gttggccccc tcttcgcggg cgttcttcac ctctttcttc gagcctggca
    52141 tgttagcttc atcacgacga taagcgcagg tgacgttact cgcgccgtgg cgcagcgcag
    52201 tacgcacaca gtccatcgcg gtgtcgccgc cgcccagtac cacgacgtta agtccggcgg
    52261 tattgataaa cggctcttcc ggtagctctt cgagccccat cacctgttta gtgttggcaa
    52321 tgaggaacgg cagcgcgtca taaacgcccg gcgtatcttc attgggtaaa cccgctttca
    52381 tggaacggta agtgcctacg ccaacgaaga ccgcgtcgta ttgttccaaa agcgaatcca
    52441 aagagacatc tttacccact tcacaattga gttcgaagtg aatccccatc gcgctgaaga
    52501 tttcccgacg gcgtgccagc agggatttat ccagtttgaa agaaggaatg ccgaaagtga
    52561 gcaagccacc gatttctgga tggcgatcgt acaccgtcac cgccacgcca ttgcgggtca
    52621 gaacatccgc acaggccagc cctgccggac ctgccccgat aatcgccacc cgcttgtcca
    52681 ctttggtgac atgacttaag tcaggacgcc aacctttcgc caacgcctga tctgaaatat
    52741 agcgttcaat gttgccgata gttaccgcgc cgtgctcatc gcgaatagta caggcacctt
    52801 cacacaaacg gtcttgcgga caaacgcgtc cggtaatttc cggcagggtg ttggtctggt
    52861 gagaaagctc gacggcggcg tcgatgtttc cggctttcac cagttcaatc cactgcggaa
    52921 tatggttatg cagcgggcag gtccattcac aaacgctgtg ctcgccgaac ttaaggcagc
    52981 gcgaggcttc ccgttgtgcc tggtcggcgc gaaatggcag ataaatttca tcaaaaccgg
    53041 ttttgcgcgc ttcaatcgcc agtttatccg gctcgccacg cgcgggcgtt gcctgcattt
    53101 gttcgacttt actcattacc ggcatttctt gtgcagcggt actggcatgc cacggttgat
    53161 gctcctgacg tgcggtgcgc aagcggcggg atttcgccat gccggagagt gcgacgtcag
    53221 tgaccagttg cagcgcgtcc gccgggcaat tctcgacaca ggcaggaccg ttttcgcgcc
    53281 ctgcacaaag gtcgcattta tgcgccgtgg cttttacttt tcctgccgcg acgggtgtca
    53341 ggacgatttg catcgtacca aacggacagg ccaccacgca ggatttacag ccaatacact
    53401 tttgctgatt gacctgaatg ctgtcatcaa cgtgactgat tgcaccatta gggcagctac
    53461 gggcgcaggg cgcatcttca caatggtgac aggtcactgc actacgttgc tgctgatgtt
    53521 tgataaccgt aattcgggga tgaaaatggt gttggctcag gacatgttgc tcatcattgt
    53581 gagccatgac acaggcgatt tcacaagcat gacaacccag acattgctga ctgttggcca
    53641 taataaaacg attcataacg accttctttt ttggttgtaa aaaccttatt ctttatatga
    53701 gtgttgttat tacccgactt acaggggaac cggcaatgtt catgtgccca gaataagtaa
    53761 ctatttcgct ataaactgtg gcagatcaaa taatcccatc actgactaaa ttgcgtttca
    53821 gcgaactgga acattaatga ttttttgtgg agaagacgcg tgtgattgtt aaacgccccg
    53881 tctcggccag tctggcccgg gccttttttt acattgtgct gctgtcgatt ctttccacgg
    53941 gtatcgctct gctaactctg gcgagcagtt tgcgcgacgc tgaggctatc aatattgccg
    54001 gatcgctgcg tatgcagagt taccgcctgg gctacgactt gcaaagtggc agtccacaac
    54061 tcaatgcaca tcgccagttg tttcagcagg cactgcattc accggtatta accaacctca
    54121 acgtctggta tgtgccagaa gcagtaaaaa ctcgctatgc gcatctgaat gccaactggc
    54181 tggagatgaa taatcggctc agcaagggcg atttgccgtg gtatcaggcc aatattaata
    54241 attatgttaa tcagatagac ctgttcgtgc tggctttaca gcactacgct gaacgcaaaa
    54301 tgctgctggt ggtggcgatt tccctggctg gcggtatcgg tattttcacg ctggtctttt
    54361 ttactttgcg ccgcatacgc catcaggtgg ttgccccgct gaatcagctg gttaccgcca
    54421 gtcagcgtat tgaacacggg cagttcgact cgccgccgct ggataccagt ctgccgaatg
    54481 agcttggtct gcttgcaaaa acctttaacc aaatgtcgag cgagctgcat aaattgtacc
    54541 tttcgctgga ggcgtcagta gaagaaaaga cccgcgatct ccacgaggcc aagcgtcgtc
    54601 tggaggtgtt gtatcagtgc agtgttccca ggcgctgaac accagccaga ttgatgtgca
    54661 ttgtttccgc catattttgc agattgttcg cgacaatgaa gcagctgaat atctggagct
    54721 gaacgttggg gataactggc ggattagcga agggaagcca aaccctgagc agccaatgca
    54781 gattttaccg gtgacaatgc aagagacggt ttacggcgaa ctgcactggc aaaatagtca
    54841 cgtttcatca tcagaaccgc tgcttaacag tgtttcatcg atgctgggac gcggtttgta
    54901 ctttaatcag gcgcagaagc attttcagca gttattgttg atggaagaac gtgcgaccat
    54961 cgcccgcgaa ttgcacgact cgctggctca ggtgctttct tacttacgta tccagttgac
    55021 gttactgaag cgttcgatac cggaagataa cgccaccgca caaagtatca tggccgattt
    55081 ttcccaggcg ttgaatgatg cttatcggca gttacgcgag ctgttgacta ctttccgcct
    55141 gacgctgcag caggcggatc tcccctccgc gttgagggaa atgctggata cgttacaaaa
    55201 tcaaaccagc gctaaactga ccctcgactg ccgtctgcca accctggcac tggatgcgca
    55261 aatgcaggtg catttgttgc aaattattcg cgaagcgggg ctgaatgcga tgaagcacgc
    55321 caacgccagc gaaatcgcca tcagttgcgt caccgcgccg gacggcaatc acacggttta
    55381 tatccgtgat aacgggattg gtatcggtga accgaaagaa cccgaaggtc attatggact
    55441 gaatatcatg cgcgaacgcg cggaacggct gggcgggacg ctgacttttt cacaaccttc
    55501 cggcggcggc acgttagtga gtattagctt tcgctccgca gagggtgagg ggaaggggaa
    55561 cacgccggct gaggtgtaaa taattaccct cacaccggcc ctctcccttt aaaggcgaga
    55621 gggaaaaacg tgcccatatt gacctgtaag cgagtgtgtc agcaatctca tgataattta
    55681 cattaactcc tttttttctc cacgattggc tcgtaccttg ccgctacagt gaagcaagtc
    55741 aagcctacaa caatacgcag aaacacgagg tcctctttta atggcgaatt tctttattga
    55801 tcgccccatt tttgcctggg tgctggcaat cctgttgtgt ctgacaggta ccctggcgat
    55861 tttttcattg cccgttgaac aataccccga tctcgcgcca ccgaatgtgc gagtgaccgc
    55921 taactatccc ggcgcatcgg cccagacgct ggaaaacacc gtgacccagg ttatcgagca
    55981 aaatatgacc ggcctcgata atctgatgta tatgtcatct cagagcagtg gcaccggtca
    56041 ggcatctgtc actttaagtt ttaaagcagg caccgatccg gacgaagccg tgcagcaagt
    56101 acaaaaccag ctgcaatcag ccatgcgaaa gttaccgcag gcggtgcaaa atcagggcgt
    56161 gacggtgcgt aaaaccggcg ataccaacat tctgaccatt gccttcgtct ctaccgatgg
    56221 ctcgatggat aaacaggata ttgctgatta tgttgccagt aatattcagg acccgttaag
    56281 ccgcgtgaat ggcgtcgggg atatcgatgc ctatggttcg caatattcca tgcgtatctg
    56341 gctggacccg gcgaaactca acagtttcca gatgacggct aaagatgtca ctgatgccat
    56401 tgagtcacag aacgcgcaga ttgcggttgg gcaacttggt ggtacacctt ccgtcgataa
    56461 gcaggcgctc aacgccacca ttaacgccca gtcactgctg caaacaccag aacagttccg
    56521 cgatatcacc ttgcgggtga atcaggacgg ctcagaggta aggctgggcg atgtcgccac
    56581 cgtcgaaatg ggggcggaga aatacgatta tcttagccgc ttcaatggta agccagcctc
    56641 cgggttgggg gtaaaactgg cctccggcgc aaacgaaatg gcgacagcgg agctggtgct
    56701 caatcgtctc gacgagctgg cgcagtattt cccgcacgga ctggaataca aggtggcgta
    56761 tgaaaccacc tcgtttgtta aagcctccat tgaagacgtg gtgaaaacgc tgctggaagc
    56821 tatcgctctg gttttcctcg ttatgtatct gttcctgcaa aacttccgcg ccacgctgat
    56881 acccactatc gccgtgccgg tggtgttgat gggaaccttc tccgtacttt acgccttcgg
    56941 ttacagcgtc aacaccttaa ccatgttcgc gatggtgctg gcgatcggtc tgctggtgga
    57001 tgacgccatc gtggtggtgg aaaacgtcga acgtattatg agtgaggaag gactcactcc
    57061 tcgcgaagcc acacgtaaat cgatggggca gatccagggg gcactggttg ggattgcgat
    57121 ggtgctttcg gcggtatttg taccaatggc cttcttcggc ggcaccaccg gtgccatcta
    57181 tcgccagttc tctattacca ttgttgcggc gatggtgctg tcagtactgg tagcgatgat
    57241 cctcactccg gctctgtgtg ccacgctact taagccactg aaaaaaggtg agcatcacgg
    57301 gcaaaaaggt ttttttgcct ggtttaacca gatgtttaac cgcaacgccg aacgctacga
    57361 aaaaggggtg gcgaaaattc tccaccgtag cctgcgctgg attgtgattt atgtcctgct
    57421 gcttggcggc atggtgttcc tgttcctgcg tttgccgacg tcgttcttac cgctggaaga
    57481 ccggggcatg tttactacct cggtacaatt gcccagcggt tcaacccaac aacagaccct
    57541 gaaagtcgtt gagcaaatcg agaaatatta cttcatccat gaaaaagaca acatcatgtc
    57601 ggtgtttgcc accgttggtt ctggccctgg gggtaacggg caaaacgtgg cgagaatgtt
    57661 tatccgcctg aaagactgga gcgaacgcga cagtaagacc ggcacctcgt ttgccattat
    57721 cgagcgtgca acgaaggcgt ttaaccaaat taaagaagct cgcgttatcg ccagcagccc
    57781 accagcaatt agcggtcttg gtagttctgc agattttgat atggagttgc aggaccacgc
    57841 tggagcgggt cacgatgcgc tgatggcagc acgtaatcag ttgctggcgc tggcggcgga
    57901 aaacccggag ctaacccgtg tgcgccataa cggcctcgat gacagtccgc agttgcagat
    57961 tgatatcgac cagcgtaaag ctcaggcgct gggcgttgat atcgacgata ttaacgacac
    58021 actgcaaacc gcctggggtt cgagctatgt gaatgacttt atggatcgcg gtcgcgtgaa
    58081 gaaagtctat gtgcaggcag ctgcgccgta tcgcatgctg ccagatgaca tcaatctctg
    58141 gtatgtccga aataaagatg gcggcatggt gcccttctct gctttcgcga cctcacgctg
    58201 ggaaacaggc tcgccgcgtc tggaacgcta taacagttat tctgcggttg agattgttgg
    58261 ggaagccgca ccgggggtca gtaccggtac ggcgatggat attatggaat cgttagtgaa
    58321 gcagctgcca aacggctttg gtctggagtg gacggcgatg tcgtatcagg agcggctttc
    58381 cggcacgcag gctccggcgc tgtacgccat ttccttgctg gtggtattcc tgtgtctggc
    58441 ggcattgtat gaaagctggt cggtaccgtt ctcggtaatg ctggtcgtgc cgctgggggt
    58501 aatcggcgcg ctgctggcaa cctggatgcg cgggctggaa aacgacgttt acttccaggt
    58561 gggcctgtta acggtcattg gtttatcggc gaaaaacgcc atcctgatcg tcgagtttgc
    58621 taacgagatg aaccaaaaag gccacgacct gtttgaagcg acgctccacg cctgccgtca
    58681 gcgtttacgc ccgattctga tgacctcgct ggcatttatc ttcggcgtat tgccaatggc
    58741 aaccagcacg ggtgccggtt ccggtggtca gcatgcggtg ggtactggcg taatgggcgg
    58801 gatgatttca gctactattc tggctattta cttcgtgccg ctgttctttg tgctggtgcg
    58861 ccgccgcttc ccgctgaagc cgcgcccgga ataagcaata aaaaaggcga catgccaatg
    58921 tgtcgccttt ttcaactttc cgattaagaa cctgctcagc gggttcttgc tgtttatact
    58981 ttgtctcagg aattacttac gaagcataac ttcgataaag tctttccagt tccccagttc
    59041 acgttcaatc ataacaacct ctcttataat tatgggtatt ctacggaaaa aatataccgt
    59101 ggtgaagcta atttactcga ttgctgcgat gactacctcc gggggacaaa ccttatgtaa
    59161 atactatggt cctgcggtga taatttgtat gtgatacaca gcaacatttc gagatattca
    59221 tacggcatct aatacttatt taattctggt taaaatacag acagataaca agatgaatat
    59281 tcttaatgtt tacgttaaaa atgtttaata ttatttaata gttgttaatt tgaatacttc
    59341 gataatgtta tattttctga taatcatttg caggcaaaat gttttcaccc ttaaatgagt
    59401 atttattctc ataaatcgaa aaaggattca ttatggttac actttacggc atcaaaaatt
    59461 gtgacaccat taaaaagact cgccgttggc tggaagccaa taacatcgac tatcgttttc
    59521 atgattaccg cgtcgatggg ctggacagcg aattattgaa cggttttatc aacgaattag
    59581 gctgggaagc gttactcaac acccgtggta caacctggcg taaactggac gaaactaccc
    59641 gcaataaaat caccgatgcg gcctctgcgg cggcattaat gactgaaatg cctgcaatta
    59701 tcaaacgtcc attgctctac gcgcctggta agcctatgct gctgggtttc agtgattcca
    59761 gttatcagca atttttccat gaggtgtagt ctatgtcgtg cccggttatt gagctgacac
    59821 aacagcttat tcgccgccct tccctgagtc ctgatgacgc aggatgtcag gctttgttga
    59881 ttgaacgttt gcaggcgatc ggttttaccg ttgaacgcat ggattttgcc gatacgcaga
    59941 atttttgggc atggcgtggg caaggtgaaa cgttagcctt tgccgggcat accgacgtgg
    60001 tgccgcctgg cgacgccgat cgttggatca atccgccgtt tgaacccacc attcgtgacg
    60061 gcatgttatt cgggcgcggt gcggcagata tgaaaggctc gctggcggcg atggtggtag
    60121 cagcagaacg ttttgtcgca caacatccca accatacggg gcgactggca tttctgatca
    60181 cttctgatga agaagccagt gcccacaacg gtacggtaaa agtcgtcgag gcgttaatgg
    60241 cacgtaatga gcgtctcgat tactgcctgg tcggcgaacc gtcgagtatc gaagtggtag
    60301 gtgatgtggt gaaaaatggt cgtcgcggat cgttaacctg caacctcacc attcatggcg
    60361 ttcaggggca tgttgcctac ccacatctgg ctgacaatcc ggtacatcgc gcagcacctt
    60421 tccttaatga attagtggct attgagtggg atcagggcaa tgaattcttc ccggcgacca
    60481 gtatgcagat tgccaatatt caggcgggaa cgggcagtaa caatgttatt ccgggtgaac
    60541 tgtttgtgca gtttaacttc cgcttcagca ccgaactgac tgatgagatg atcaaagcgc
    60601 aggtacttgc cctgcttgaa aaacatcaac tgcgctatac ggtggattgg tggctttccg
    60661 ggcagccatt tttgaccgcg cgcggtaaac tggtggatgc ggtcgttaac gcggttgagc
    60721 actataatga aattaaaccg cagctactga ccacaggcgg aacgtccgac gggcgcttta
    60781 ttgctcgcat gggggcgcag gtggtggaac tcgggccggt caatgccact attcataaaa
    60841 ttaatgaatg tgtgaacgct gccgacctgc agctacttgc ccgtatgtat caacgtatca
    60901 tggaacagct cgtcgcgtga tgagtggttc tgcaagagga aataagcatg gactggctgg
    60961 ctaaatattg gtggattctg gtgattgtct ttttggtcgg cgtcctgctg aacgtgatta
    61021 aagatctcaa gcgcgtcgac cataagaaat ttctcgccaa caagccggag cttcccccgc
    61081 atcgtgattt caacgataag tgggacgatg acgacgactg gccgaaaaag gatcaaccga
    61141 agaagtaagc tgagtgctca accgtaggcc ggataaggcg ttaacgccgc atccggcaat
    61201 ggtgaacgat gcctgatgcg acgctcacgc gtcttatcag gccaggtaat ctgtctttta
    61261 catcatttca atcacatcat catcgccagg cttaccgcca cttagcgctt ggtcgaaata
    61321 atgtttcgga atggtataac gcaagtgatc cagggcaaat tgcatactgc gattgtcgat
    61381 cgcatggcct aaatcctcca cgatatccag cgtcacgtca ccaccggcac tgattaacgc
    61441 ttcctgcgcc gcaactgcat gcgccagatc gataaccgga tcttcaccac catgaatcaa
    61501 gtgaattgtc gtggctgtag acgcggtctc cggcaggctc gcatagcgtc cgttaaaggc
    61561 aatgacgcgc gaagcaaggc ccggttcggc tttaatgctc tccagcgcca taatcgcgcc
    61621 ttgtgagaaa ccaattagcg ccgtggcgtt agcccctact ccgctctgtt tctgccagta
    61681 gcgtaccgtt tcaataaacg tcggcatgat ggcatccaca cgcgcctggc gattatcttc
    61741 cgtaataccc tgcactgaaa accactgacg ccccgccgga ttaccgctcg gctccgcgcc
    61801 accgacactc accaccagcg catcaggaaa cagcggtgca aaccagctgc ctatttcccc
    61861 cattgccacc gggttatccc cgacaccgtg gaaaagcagt aacaactgtt gtgcaggttt
    61921 atccgggctt tgaacaacaa aatggtcatg tttcatggcg aactccttaa ctgatgtcat
    61981 taattttacg ccgctgactc acaatgacca tgggagaaaa ttgaaggagt cagtgaaaaa
    62041 attgccattg cgttatgcga tcgcgcagac gctcagtgcg aacctcattt agtgcgaaca
    62101 gcgcctgcgc ggcctcttcc cgctggcgaa ccagtaacat cttgcggcct gaaagtttaa
    62161 gtgtggtaca taactgcgcg tcactggcgt ttttctgtaa acgcccacgc agcgccggta
    62221 atgccagttc actagtttgt agcagacgca ataagcaacc taacgatgtt aatagcggac
    62281 gatgagcgaa agcaaaaccg gccagttcaa gccagtcgtc gtcagaaagg atggcatcgt
    62341 ttagtggatc aacaggaagc atttcgccat tccactgcgc gagagcttgc gcatcgcgac
    62401 gtaaacggta atgctcacgt tcagccagct gtttacccgc atcactcatc ggtaacagcg
    62461 ccatcgccgt atagcaaccg ctgctggctt cccgatgatt acccatccgc accagcacaa
    62521 aaccgcagcg ttgccagaaa cgccataact ccccagtgta accaaaactc accgaaagat
    62581 agtcgaggtc atgcgtatat tgcaaagctc cagcaataag tttccgccct gtgccttccc
    62641 gctgacgagc cggatgaact gctatccggc taacccgcct tccgcgcaat gtcgccgcca
    62701 gtggattgct gccgtgcgcc gccagcgact gggccaccag attaccccgc gggcgacgaa
    62761 aacctgccca taccgcctga ctgagttctt gagataatcc gccctcatcc accagccaca
    62821 acgccccggc aatctcgttt tcgccagccg cctgtaaaaa atgttgccct ggtgcatcca
    62881 tcatccggcg taaatccagc ggcgaggtcc ggtagtgcgc accagacaat aactgataaa
    62941 cctttaacgg cgtttctggc tcgcttcgcc ataacgtttg ttcaaatgcg gaaatgacga
    63001 tattgccttg tggtgtatgg gtgaagtttt catcgttaaa aaccagtgcc tcactaacca
    63061 ttttttccag cgggcatccc tgtgcccagc ggatcggctg ttgcagttca aaacggtgta
    63121 aatgcggaaa gcgagcgcaa aatttcagca aaaaaccacg tccggtacct tcgtagccct
    63181 gcaccgtagt ggttaacaac gttcgaggaa aacgcgatac cagttgatgc aacaacggcg
    63241 caggtatggc tgcggcttcg tcgaccacca gccagtcggc ttgctcatcg ctggctaaca
    63301 aggcatccgg tgcaataaag cgaaacttct cgcccgcaaa ttgtgccagt acatccgttg
    63361 ccgcttttgc gggcgcggtg acaatcgcac tgcccgcaat acgagaaatg agttgccctg
    63421 ccaacgccga cttaccgcgc ccacgcgcag ccgttaccgc tgccacgccc ggcggcatgg
    63481 tcagtagctg ctgtaagagt tgctgttgtt ctggttgtgg tgcgccggtc gcggggtgcc
    63541 agtcagtacg gggagtaaaa tgcgccaacg agaacggctg gttttgccgc cagaggatag
    63601 cgtcgttatc cgccgtaagt acgcgtttga gatgctggac aaaatgcggc gtcgcaatag
    63661 ggtcagggca atcactccag cgcagcgagt cggcatcagg ttggttttcc cactcttccc
    63721 atacagggag taacaaaacc agccagcttc ccgctttcaa cgttccgcta agtgctgcaa
    63781 aggcagcggc atcaaagccg tggcgggcgt cgaataccgc atgccggaac tcgcgcccaa
    63841 gtaaagtttg tagcgccgag ggtgaacagt ggttttcagc atctagctgc ggcgaaatcc
    63901 acagccagtc gccaggtaag gcatcacgca acttaagagc atgatcaaaa caccaaccct
    63961 cttctccgct caacaccagc aagcggcaga tcccttcacg tttcatttgc gctgttaatg
    64021 tgtaaagcgc agtcagttca gccatccctg ccccggaaat taaatgcttt taccaaaagt
    64081 attgcattgt gccggatcgc cgctgtcgaa accacgttta aaccagctgc agcgttgctg
    64141 agaagtgcca tgtgtgaaac tgtctggtac tactcgcccc tgactttgct gttgtaaacg
    64201 gtcatcgccg atggcctgcg ccgcattcag cgcctcttcc agatcgccag tttccagaac
    64261 gccttgctgc tgcatactat gcccccagac accggcaaaa cagtcggcct ggagttccat
    64321 acgcacagat aagcggttta cttccgcctg cgtcgcgttt tgttgcagtt gacgaacttt
    64381 cggctcgatg cctaacagtt tctgcacatg atgaccgact tcatgggcga taacgtaccc
    64441 ctgggcaaaa tcgccatccg cgccaagttt gtctttcatg tcatcataga aggagagatc
    64501 gatataaact gtgccatccg ccgggcaata gaacggcccc ataatggact ggcccgcccc
    64561 gcagccggtg cgcgtcattc cacggtacat gaccagcttc ggttgctgat aggtcttacc
    64621 catcttttcg aactgttgtc cccaggtgtc ttccgtggtt gccagaatca ccgaggtgaa
    64681 ttttgcggct tcatcttcat tagggctaat tgaccgcgtt gattgttgtt gagaaaccgg
    64741 ctgcccggtc atcaacccgg ttaaatcaac gccatagtag cctgcaacca gcacgactat
    64801 cagtaaaatc agcccgcctt taccgctcgg cagacggaaa ccgggaccgc ccattgacgg
    64861 accgccagag ctgttgcgcc tgtcttcaac attgtcactt tcacgtcgcc cttgccaacg
    64921 catagatacc tcaacaatat attcattata gaaatgatcg taggtggttc agaggaagat
    64981 taccacagga aagcgggatg aaatggcgga ggaatgagcg ttcaatgcat atattccgag
    65041 agcggttgtc ttgccgtgcc agctgcacgg caagatgatg aacagaaaaa tcagtccagc
    65101 tgtacaccca ggcggtgggc gacggcttca taggcttcga tcaggccacc gagactctgg
    65161 cggaaacggt ctttgtccat tttctccagt gtttctttgt cccacaggcg gctgccgtcc
    65221 ggggagaact catcgcccag taccacttca cctttgtaca gaccaaactc cagcttgaag
    65281 tcgaccagaa tcagaccagc atcatcgaac agttttttca gcacgtcgtt cgctttgtag
    65341 gtcagctctt tcatacgcgc caggttctct ttgctcaccc agccaaaggt ttcgcagtaa
    65401 gattcgttga ccatcggatc gtgcatggca tcgtttttca ggaacagatc gaacagcggc
    65461 gggttcagct caataccttc ttcgattcca agacgtttca ccagagagcc agcagcacgg
    65521 ttacgcacga cacactcaac cggcaccata tccagctttt tcaccagaca ttcggtatcg
    65581 gagagcagac gctccatttg agtcgggata cccgcttcag ccagtttgct cataatgaag
    65641 tagttgaact tgttgttcac catacctttg cgatcaaact gctcaatgcg cgcgccatcc
    65701 cctgctgacg tatcattgcg gaattcgagc accaacaggt ccgggttttc cgtgctgtat
    65761 acggttttcg ctttaccacg atacaactca gcttgctttt gcatctttat cactcctggg
    65821 tgtgaattaa cgttttaaaa tcttttgctg tctggtgtgc cggatgtttt ttcggatgcg
    65881 gcgtgaacgc cttatccgaa ctacgtccga caaacaaatt tcgtgcgaat taccgctaaa
    65941 atcgcgtttt cctgccgacg cacacgtttg cgtatcatat cagaaaaaag ggccggatga
    66001 ttccagccct gtatttttac ttgctaaacg cagcctggaa gacagctacc agcgcgtcgt
    66061 tctgactctg agtcagagta tgacctttcg gatcgatgaa ctgtaggctg ctgcggttat
    66121 ctaaatcgcc aacctgcagt ttatagtcac cggatgccag gcctggatcg ctcgcgccca
    66181 gttcctgcca gtcgctgtca gacagcggct tataagttac ggccatgttg ccctgcgaac
    66241 gggtgctgtc ggtcactttc atgcccactt tttccagcga cgctggcaga cgttgccaaa
    66301 ccacattgaa cggcccgcgt acgaccagca ttggtaaacc ggtgtcatca gctgcacttt
    66361 gtacgtccat agtggtggag gcacgatttt gcgcagcgtt cgcggcgtca gtggcagatt
    66421 tatccagacc ggcggaaata acgttcatca tctccgtgct gtaacgctgc atggaagccg
    66481 cgtctgcaac cggtttgccc gcctgttcca ggttcagcag tttaaccgta accgcctgct
    66541 gataaccctg cggcttaaca gagatttgat aacgaccacg atactgctcg tcttcgtcca
    66601 gacggttcca ttgtacccaa tcggtggtca gtgtctgacc agcatcatca cgttgggtga
    66661 tggtgtagtt tttcgcctgc agcacgctaa ccacctgcgg ccacagagta ttgccacgac
    66721 cattttccac cagcaatgaa gcggtatcgc ccgtgaactg ggtacgcgcg ccagaaacca
    66781 gtgccagcgg ctgggctggt ggacgaatgt ccagcgcctt accgacagca ccactaccgt
    66841 tggtcaccgg gattgcataa tcaccggagg tcaccggcaa aatcattcca gccggggcat
    66901 gaagctccgc aagcggtgcc gcttccaggt aggcttcatc accactgacc tgacgcttat
    66961 agcgtgagtc agaactacag gcagcgagta ataaaacaag cgaaacaccc gcaacctttg
    67021 ccaggcgcga cttttgaaca gagtaagcca tcaaatctcc ctaaacttta cagcaaaccg
    67081 gcatgcttaa gcgccgctct gaccgtctca cgaccactgt cggtgattgg tgtcattggc
    67141 aggcgcagcg tatcggtcgc cacaagaccc agttccttac atgcccattt caccgggatt
    67201 ggattgggtt cgacaaatag tttgttgtgt aatggcatca gacgctgatt aataacgcgt
    67261 gcctcggcaa aatgcccttc tgctgccagt ttgcacatcc gggccatatc acgcgctgcg
    67321 acgttagccg taacggaaat aaccccatga ccgcccaatt gcatgaagtc cagcgcgctc
    67381 gcatcatcgc cgctcagcag aacaaaatca tctgaaacca gctctttgat ctggtttaca
    67441 cgcgttaagt tccctgttgc ctctttgatt ccgataatat tttttacttt cgccagacgg
    67501 cccaccgttt ccgggagcag atcgcagcca gtacgggacg gcacattata cagaatttgc
    67561 ggcaggtcag tatgctcagc gatggctttg aaatgctgat acaaaccttc ttgcgacgga
    67621 cgattgtagt aaggggttac cgtcaggcag ccgacgatac cactgtcatt gaagcgctgc
    67681 gtcaggctaa tggcttccgc agtagcgtta gcgccggtcc cggcaattac cggaatgcgc
    67741 ccatcagcca gatccagcgt catcatcacc acatcagcat gttcgtcatg atttaaggta
    67801 gcggactcgc cagtggtgcc aacagaaacg atcgccgaag taccgctggc gacatgataa
    67861 tcaatcagtt ttttcaagct agcccgacag acattacctt tttcatccat cggagtaaca
    67921 atcgcgacaa tacttcccgt gaacatgggc catcctctgt gcaaacaagt gtctcaatgg
    67981 tacgtttggt atggcattaa aagcaagcag acagaaccgt tctgattgtt gtatgcatga
    68041 tttttttatg ctttccttaa gaacaactca ccccttaaag gaataaccag tttgacactg
    68101 tcatcgcaac attatctggt gatcactgcg ttgggtgccg atcgccctgg aattgtgaac
    68161 accatcaccc gtcatgtcag tagttgcggc tgtaatattg aagacagtcg cctggcgatg
    68221 ctgggagaag agttcacgtt tatcatgctg ctttccggtt catggaatgc catcactctg
    68281 attgaatcaa cgttaccgtt gaaaggtgcc gaactggatc ttttaatcgt gatgaagcgc
    68341 acgacggcgc gtccgcgtcc gccaatgcca gcatctgtct gggttcaggt cgatgttgca
    68401 gactccccgc atttaattga acgcttcaca gcacttttcg acgcgcatca tatgaacatt
    68461 gcggagctgg tgtcgcgcac acaacctgct gaaaatgaac gggctgcgca gttgcatatt
    68521 cagataaccg cccacagccc cgcatctgcg gacgcagcaa atattgagca agcgttcaaa
    68581 gccctatgta cagaactcaa tgcacaaggc agtattaacg tcgtcaatta ttcccaacat
    68641 gatgaacagg atggagttaa gtaatgaatc cactgaaagc cggtgatatc gcaccgaaat
    68701 ttagcttgcc ggatcaagac ggagaacaag ttaatttgac cgacttccag ggacagcgtg
    68761 ttctggttta tttctacccg aaagccatga cccccggctg taccgtacag gcctgcggct
    68821 tacgcgataa catggatgag ttgaaaaaag cgggcgttga tgtgctgggt atcagcaccg
    68881 ataaacccga aaaactctcc cgttttgcgg aaaaagagct gcttaacttt acgctcctgt
    68941 ctgatgagga ccaccaggtg tgcgaacaat tcggcgtctg gggtgaaaag tccttcatgg
    69001 gcaaaaccta cgatggtatt catcgcatca gcttcctgat tgacgctgat ggcaaaatcg
    69061 aacatgtctt tgacgacttc aaaaccagca atcaccacga cgttgtgctg aactggctga
    69121 aagaacacgc ctgattactt tgctccattc cgtgctggct gcgcttgcgg ccagcatacc
    69181 tcacttctcg tgatcaagat cacattctcg ctttcccctg cgacacgggt gtcgaatcca
    69241 ttttttgctg aacgttaatg accatcattt ttgtaccgtt cagaatccag ttaatacata
    69301 acttattgaa tagattgagt taatcagaat ggcacccttt atgcaatatg aaatgcaatg
    69361 tttcatatca ttttcaagga gccgacatga accgctttgt ggtggccgaa ccactgtggt
    69421 gtacaggatg taatacctgt ctcgctgcct gttcggacgt gcataaaacg caaggtttac
    69481 agcaacaccc gcgcctggcc ctggcgaaga cgtcaacaat cactgcccct gtcgtgtgtc
    69541 atcactgtga ggaagcccct tgcctgcagg tctgcccggt caatgccatc tctcagaggg
    69601 atgatgcgat ccaactcaac gaaagcctct gtattggctg caagctttgc gccgtggtct
    69661 gcccatttgg cgcaatcagc gcttcaggaa gccgtccggt gaatgcccat gcgcaatatg
    69721 tttttcaggc tgaaggctca ctcaaagacg gcgaagaaaa cgtgccgccc caacatgcgt
    69781 tgctgcgctg ggaaccgggt gtccagaccg tcgcggtgaa atgcgacctg tgtgatttct
    69841 tgccagaagg tccggcctgc gttcgcgctt gcccgaatca ggcgttacgg ctgatcaccg
    69901 acgatagcct gcaacgtcag atgaaagaaa aacagcgcct tgccgcaagc tggtttgcca
    69961 atggcgggga ggatcccctt tccctcactc aggagcaaca ctaatggatg ccctgcaatt
    70021 attaacctgg tcgctgatcc tctatctgtt tgccagtctg gcttcgctgt ttttactcgg
    70081 tctggacaga ctggctatta agctttccgg catcacatcg ctggtgggcg gcgtgattgg
    70141 catcatcagc ggaattacgc aattacatgc tggtgtaact ttagtcgccc attttgccac
    70201 cccttttgac tttgccgatt taaccctgcg aatggatagc ctctcggcat ttatggtgct
    70261 ggttatctcc ttgctggtgg tggtttgttc gctctattca ttgacttata tgcgcgaata
    70321 cgagggcaaa ggcgcggcgg cgatgggctt ctttatgaat cttttcatcg catcgatggt
    70381 tgccctgctg gtgatggaca acgctttttg gttcatcgtg ctgtttgaaa tgatgtcgct
    70441 gtcttcctgg tttctggtca ttgccaggca ggataaaacg tcgatcaacg ctggcatgct
    70501 ctactttttt atcgcccacg ccggatcggt gctgattatg atcgccttct tgctgatggg
    70561 gcgcgaaagc ggcagcctcg attttgccag tttcgcacgc tttcactttc tccggggctg
    70621 gcgtcggcgg tgtttctgct ggcctttttc ggttttggcg cgaaagccgg gatgatgccg
    70681 ttgcacagct ggttgccgcg cgctcaccct gccgcaccat cgcacgcttc ggcgttgatg
    70741 tctggcgtaa tggtcaaaat cggtattttc ggcatcctga aagtagcgat ggatctgctg
    70801 gcgcaaacgg gtttgcctct gtggtggggc attctggtga tggcgatcgg cgcaatctcc
    70861 gcgctcctgg gcgtgctata tgcgctggcg gaacaggata tcaaacggct gctggcctgg
    70921 agtaccgtcg aaaacgtcgg cattattttg ctggcagtcg gtgtggcgat ggacggtctg
    70981 tcactgcacg acccgctgct caccgtggtt ggactgctcg gcgcactgtt tcatctgctc
    71041 aaccatgcgc tgttcaaagg gctgctattt ctcggcgcgg acgcgattat ttcgcgtttg
    71101 catacccacg acatggaaaa aatgggggca ctggcgaaac ggatgccgtg gacagccgca
    71161 gcatgcctga ttggttgcct cgcgatatca gccattcctc cgctgaatgg ttttatcagc
    71221 gaatggtaca cctggcagtc gctgttctca ctaagtcgtg tggaagccgt agcgctacaa
    71281 cttgcgggtc ctattgctat ggtaatgctg gcagtcactg gtgggctggc agtaatgtgc
    71341 ttcgtcaaaa tgtacggtat tactttctgt ggtgcgccgc gcagtacaca cgctgaagag
    71401 gcacaggaag tgccaaatac gatgatcgtc gccatgctac tgctcgcggc actctgcgta
    71461 ttaattgcgc ttagtgccag ttggctggca ccgaagataa tgcatattgc ccatgcgttt
    71521 accaataccc ctcccgtcac tgtcgccagc ggaatagcac ttgtacccgg cacgtttcat
    71581 acacgggtca ctccttcatt actgttgctg ttactactgg cgatgccttt gctgccaggc
    71641 ctttactggc tgtggtgtcg ttcgcgccgc gcagcgtttc gtcgcacagg agatgcctag
    71701 gcatgcggct acggctggga aaacgcgatg gctccgtcag gcaatggcgt gatgcagccg
    71761 ctgcgtgtgg tcttctctgc gttatttcgt ctacgacaac agctcgaccc tacgctgagg
    71821 ctaaataaag gtcttgcgca cgtcaccgcc agggctcaga gcacagaacc cttctgggat
    71881 gagcgggtga tccgccccat cgtgagcgcc acccaacggc tggtcaaaga aatacagcat
    71941 ctgcaaagcg gcgactttcg tctctattgc ctgtatgtgg tcgccgcact ggttgtgctg
    72001 ctaatcgcta ttgccgtcta aggaaatcac catgagacaa actctttgcg acggatatct
    72061 ggtcattttt gcgttagcac aggccgtgat tctgctgatg ctaaccccac tttttacggg
    72121 tatttcccgg cagatacgcg cgcgtatgca ctcccgccgc gggccgggga tctggcagga
    72181 ttatcgcgat atccacaaac tgtttaaacg ccaggaagtt gcgccgacat cttcaggtct
    72241 gatgttccgc ctgatgccgt gggtattaat cagcagcatg ctggtgctgg cgatggcctt
    72301 accactattt attaccgttt ccccttttgc gggcggcggc gatctgctca cccttatcta
    72361 tcttcttgcc ctgtttcgtt ttttctttgc tctttccggg ctggataccg gaagtccgtt
    72421 tgcgggagtc ggtgccagtc gcgagttgac gctcggcatt ctggtcgaac caatgcttat
    72481 tctctcactg ctggtattgg cgctgatagc aggttccacg catatcgaga tgatcagcaa
    72541 tacgctggcg atgggctgga actcgccgct aaccaccgta ctggcgttac tggcctgtgg
    72601 ttttgcctgc ttcattgaga tgggaaaaat tccctttgat gttgctgaag cagaacagga
    72661 attacaggaa ggcccgctga ccgaatattc cggtgccggg ctggcgctag cgaaatgggg
    72721 gcaggggctg aaacaggtcg tgatggcagc actgtttgtg gccctgtttc tgccctttgg
    72781 gcgcgcgcaa gaactttctc tcgcctgcct gctgacttca cttgtcgtta cgctgctcaa
    72841 ggttttgctg atttttgtac tggcctcaat cgcagaaaac acgctggcac gcgggcgttt
    72901 tttactcatt caccatgtga cctggcttgg cttcagcctt gctgcgcttg catgggtctt
    72961 ctggttaacc ggtctgtaag gagcactgac ggaatatgga aaatcttgct ctgacgacgt
    73021 taatgctgcc ttttatcggc gcactggtcg tttcgttttc gccacaacgt cgggccgccg
    73081 aatggggggt tttgttcgcc gcgctgacca cgctgtgcat gttgtcgctg atctccgcgt
    73141 tttatcaggc cgataaagtt gccgtcacgt tgacgttggt caacgtgggg gatgtggcat
    73201 tgtttggcct ggtcattgat cgcgtgagta cgctgattct gtttgtggtg gtgtttctcg
    73261 gtttgctggt cacgatctac tccacgggtt atctgacgga taaaaatcgc gaacacccgc
    73321 ataacggcac gaatcgttat tacgcatttt tgctggtgtt tatcggcgcg atggcgggac
    73381 tggtactctc ctcgacgctg ctcggtcagt tgttgttttt tgaaattacg ggcggctgct
    73441 cctgggcgtt gatcagttat taccagagcg ataaagcgca gcgttcagca ctaaaagcgt
    73501 tgcttatcac ccatatcggt tcgttggggt tgtatcttgc cgccgccacg ctgtttttgc
    73561 agaccggaac gtttgtgctt agcgcgatga gcgagttaca tggcgacgca cgttatctgg
    73621 tttatggcgg catcctgttt gccgcgtggg ggaaatcggc ccagctaccg atgcaagcgt
    73681 ggctaccgga cgcaatggaa gcgccaacac cgatcagcga ctatctccac gccgcatcga
    73741 tggtgaaagt gggcgtttac atttttgccc gcgccattat cgacggcggc aatatcccgc
    73801 atgtgattgg cggcgttggc atggtcatgg cactggtcac cattctttat ggctttctga
    73861 tgtatttgcc acagcaggat atgaagcggt tgctggcctg gtcgaccatc actcaacttg
    73921 gctggatgtt cttcggcttg tcgctctcca tcttcggctc gcggctggcg ctggagggta
    73981 gcatcgccta catcgtcaac cacgcgttcg ctaaaagcct gtttttcctt gtagcaggtg
    74041 cgctgagtta cagctgcggc acgcgcttgt tgccgcgtct gcgtggcgta ttgcacaccc
    74101 tgccgttgcc aggcgtgggt ttctgcgtgg cagcgctggc aattaccggt gtgccgccgt
    74161 tcaacggctt cttcagtaaa ttcccgctgt ttgccgccgg ttttgcgttg tcagtggagt
    74221 actggatcct gctgcccgcc atgattctgc tgatgattga atcggtcgcc agtttcgcct
    74281 ggtttattcg ctggtttggt cgcgtcgtgc cgggcaaacc gagcgaggcc gtcgccgatg
    74341 ccgcaccgct gccagggtca atgcgcctgg tgttgattgt actgattgtg atgtcgctga
    74401 tttccagcgt aatcgccgcg acctggttgc agtaaggaga tgatgaatga ccggttctat
    74461 gatcgtaaat aatctggcgg gactgatgat gctgacatcg ctgtttgtga ttagcgtcaa
    74521 aagctatcgc ctgtcatgcg gattttaggc ctgccagtcg ctggtgctgg tgtctatttt
    74581 cgccactctc tcgtgcctgt tcgccgcaga gcaactgctg atctggtccg ccagcgcctt
    74641 tatcaccaaa gtgctgctgg taccgttaat catgacttac gctgcacgaa atattcccca
    74701 gaacatcccg gaaaaagcgt tattcggtcc ggcaatgatg gcactgctcg cggcgttaat
    74761 tgtcctgctt tgcgcatttg tcgttcagcc cgtgaagcta ccgatggcta ccgggctgaa
    74821 accggcgctg gcggtagcgt taggtcattt tctgcttggc ctgctgtgca ttgtcagcca
    74881 gcgcaatatc ctgcggcaaa tttttggtta ctgcctgatg gaaaacggct cccatctggt
    74941 gctggcgctt cttgcctggc gagcaccgga actggtggaa ataggtatcg ctaccgacgc
    75001 catcttcgcc gtcattgtga tggtgttact ggcaagaaaa atatggcgta cccacggcac
    75061 gctggacgtg aacaacttga ccgcgctgaa gggataatga gatgagttat tctgtgatgt
    75121 tcgctttact cctgctcacg ccgctgcttt tttcgctgct ctgttttgcc tgccggaaac
    75181 gggggctctc tgcgacttgt acggtgactg tattacatag cttagggatc acactgctgc
    75241 tgattctggc actctgggtg gtccaaactg ccgctgatgc aggagaaata ttcgctgcgg
    75301 gactgtggct tcatattgat ggtctgggcg gtttgttcct cgccattctt ggtgtgattg
    75361 gctttctcac cggtgtttac tcgattggct acatgcgtca tgaagtggca cacggcgagc
    75421 tttcacccgt tacactgtgc gattactacg gtttcttcca tctgtttttg ttcaccatgc
    75481 tgctggttgt taccagcaat aacctgattg tgatgtgggc ggcgatcgaa gccaccacct
    75541 taagctcggc gtttctggta ggcatttacg gtcagcgttc atcgctggaa tcagcatgga
    75601 agtacatcat tatttgtact gttggtgtcg cttttggtct gttcggtacc gtgctggtat
    75661 acgccaacgc cgccagcgtt atgccgcagg cagaaatggc gatattctgg agcgaggttc
    75721 ttaagcaatc gtccttgctt gacccaacat taatgctgtt ggcctttgtg tttgtgctaa
    75781 ttggctttgg taccaaaacc gggctatttc ccatgcacgc ctggctgccg gatgctcaca
    75841 gtgaagcgcc gagtccggtc agcgccctgc tctccgccgt attgctgaac tgcgcgctgt
    75901 tggtgctgat tcgctattac atcattattt gccaggccat cggcagcgat ttccccaacc
    75961 ggttgttgct catcttcggc atgttgtcgg ttgccgtggc ggcatttttc attctggtac
    76021 agcgggacat taagcgtctg ctggcgtact ccagcgtgga gaacatgggg ctggtcgcgg
    76081 tggcgctagg cattggcggg ccgctgggaa tttttgccgc gctgctgcac accttaaacc
    76141 acagtctggc aaaaacgctg ctgttctgcg gttccggcaa tgtactgctc aagtacggca
    76201 cgcgcgatct caacgtcgtc tgtgggatgc tcaaaatcat gccatttacc gccgtgctgt
    76261 ttggcggcgg tgcgctggcg ctggcaggga tgccgccctt caacattttt cttagcgaat
    76321 ttatgaccgt taccgcagga ctggcacgta atcacctgct gattatcgtc ctgctgttat
    76381 tgctgttaac gctggtgctc gcgggcctgg tacggatggc tgcgcgggtg ttaatggcga
    76441 aaccgccgca ggccgttaac cggggtgatc tcggctggtt gaccacctcg ccaatggtga
    76501 ttctgctagt catgatgctg gcgatgggaa cgcatattcc acaacctgtc atcaggatcc
    76561 tggcgggcgc ttccactata gtcctctcag ggacgcacga cctgcctgca caacgtagca
    76621 cctggcatga ttttttgcct tcaggcaccg catctgtttc ggagaaacac agtgaacgtt
    76681 aattcatcgt caaatcgtgg cgaagcgatt ctcgccgccc tgaaaacgca gttccccggc
    76741 gcggtgctgg atgaagagcg acaaacgcct gaacaggtca ccattacggt gaaaatcaat
    76801 ctgctgcctg acgttgtaca ttatctttat tatcaacatg atggctggct tccagtcctg
    76861 tttggcaacg acgagcggac acttaacggt cattacgcgg tttattatgc cctttcaatg
    76921 gaaggggccg aaaaatgctg gatcgtggtg aaggcactgg tcgatgccga cagtcgggag
    76981 tttccgtcag tcacaccgcg cgtccctgcc gcggtctggg gcgagcgaga aattcgcgat
    77041 atgtacgggc tgattccggt tggcctgccg gatcagcgtc gcctggtgtt gcccgatgac
    77101 tggccggaag atatgcatcc gctgcgcaaa gatgcgatgg attatcgact gcgccctgaa
    77161 ccgacgacag attccgaaac gtatgcgttt atcaatgagg gcaacagcga tgcgcgggtg
    77221 atccctgtcg gcccgctgca tatcacctcc tatgaaccgg gtcacttccg cttgtttgtg
    77281 gatggcgagc aaattgtcga tgctgattac cgcctgtttt atgtccatcg cggcatggag
    77341 aaactggcag aaacgcggat gggctacaac gaagtgacct tcttatcgga ccgcgtgtgt
    77401 gggatttgcg gttttgccca cagtgtggcc tataccaact cggttgaaaa tgcactgggg
    77461 attgaggtgc cgcaacgagc acatactatt cgctcgattc tgctggaagt cgagcggcta
    77521 cacagtcatt tgctcaacct tggcctctcc tgccattttg ttggttttga taccggcttt
    77581 atgcaatttt tccgcgtgcg ggaaaagtcg atgacgatgg cggaattgct gaccgggtcg
    77641 cgtaaaacct acggtctgaa tctgattggt ggtgttcgcc gcgatattct caaagagcaa
    77701 cgtctgcaaa cgctgaaact ggtgcgcgag atgcgcgccg acgtgtcgga gctggtagag
    77761 atgctgcttg ctacgccgaa tatggaacaa cgcactcagg gcattggcat tctcgaccga
    77821 caaatcgccc gtgattatag ccctgtaggg ccgctgatcc gcgcagtggt tttgcccgtg
    77881 atttgcgctt tgatcacccc tacgccgact acggcaatat tccaaaaaca ctgtttacct
    77941 ttaccggcgg cgatgttttc tcccgcgtga tggtccgtgt caaagagacg tttgattcgc
    78001 tggcaatgct ggaatttgcc ctcgacaaca tgccggatac cccactgctg accgaaggct
    78061 ttagctataa acctcacgca ttcgcgctgg gctttgttga agcgccacgc ggtgaagacg
    78121 tgcactggag catgctcggt gataaccaaa aattgttccg ctggcgctgt cgtgccgcca
    78181 cctacgccaa ctggccggtg ttgcgttaca tgctgcgcgg caataccgtt tctgacgcac
    78241 cgctgattat cggtagcctt gatccctgct actcctgtac cgaccgtgtg acgctggtag
    78301 atgtgcgcaa gcgccagtca aaaaccgtgc tgtataaaga gatcgaacgc tacggcattg
    78361 atcgtaaccg ttcgccgctg aagtaaggac agaagatgct gaagttactg aaaactatta
    78421 tgcgcgccgg aaccccgacg gtgaaatatc ccttcgcgcc actggaggtc agcccaggct
    78481 ttcgcgggaa accggacctg atgcccagcc aatgtattgc ctgcggtgcc tgcgcctgtg
    78541 cttgtccggc aaatgcgctg actatccaga ccgacgacca gcaaaattcg cgcacctggc
    78601 agctctatct ggggcgttgt atttactgcg gacgttgtga agaagtgtgc ccgaccagag
    78661 ccatccagct taccaataac tttgaactga ccgtcaccca taaagccgat ctctataccc
    78721 acgcgacgtt ccatctacaa cgttgcagcc gttgcgaacg cccgtttgcc ccgcaaaaaa
    78781 ccgtcgcact ggctgctgaa ttgttggcac agcaacaaaa tgctccacaa aaccgcgaaa
    78841 tgttatgggc gcaagcgagc gtctgcccgg agtgcaaaca acgcgcgacg ctgatcaacg
    78901 acgatacaga tgtaccgctg gtggctaagg agcagctatg agtccagtgc ttacgcaata
    78961 tgtcagccag cccatcacgc tggacgagca aacgcaaaag atgaagcggc atttgctaca
    79021 ggatatccgt cgctcggctt acgtttatcg catcgattgc ggcggctgca acgcctgtga
    79081 aatcgaaatt tttgctgcca tcacccccgt attcgacgca gaacgttttg gcattaaggt
    79141 tgtttcatca ccgcgtcacg ccgatatttt gttatttact ggcgcagtca cccgggcgat
    79201 gcgtatgcct gcacttcggg cgtatgagtc tgcccccgat cataaaatct gtgtttccta
    79261 cggcgcgtgc ggtgtcggcg gcggtatttt ccacgatctc tacagcgtct ggggcggcag
    79321 cgacaccatt gtccccattg atgtttggat ccccggctgc ccgccaacac cggccgccac
    79381 cattcacggt ttcgccgtgg cgctcggttt gctgcaacag aagattcacg cggtggatta
    79441 tcgcgatccc accggggtga ctatgcaacc gttgtggccg cagatcccgc catcacagcg
    79501 tatcgccatt gagcgagaag cgcggcggct ggcgggctat cgtcaggggc gagaaatttg
    79561 cgatcggctc ctgcgccatt taagcgacga tcctacagga aatcgggtta acacctggtt
    79621 gcgcgatgcc gacgatccac gtctcaatag tatcgttcag caactctttc gcgtactccg
    79681 ggggttacat gactgaagag tgcggggaaa ttgttttctg gacgctgcga aaaaagtttg
    79741 tcgccagtag cgacgagatg ccggaacaca gctctcaggt aatgtattac tcgctggcta
    79801 tcggtcatca cgttggcgtg attgattgtc tgaatgtcgc cttccgctgc ccactgacgg
    79861 aatacgaaga ttggcttgca ctggtcgaag aggagcaagc ccgacgtaag atgctggggg
    79921 tgatgacttt tggtgagatt gttattgacg ccagccacac cgccctgttg acccgggcat
    79981 tcgcgccact ggcagatgac gcgacgtctg tgtggcaagc gcgtagcatt caattcattc
    80041 atctgttgga tgaaattgtg ctggaaccgg ctatctatct gatggccaga aaaattgcgt
    80101 gagaaggatt tctcattaat aaggactgtt gatggctatg tcagacgaag cgatgtttgc
    80161 cccgccgcaa ggaataacaa ttgaagcggt aaacggaatg ctcgcggagc ggttagcgca
    80221 gaaacacggc aaggcgtctt tattacgcgc cttcatcccg ctgccgccgc cgttcagccc
    80281 ggtacaactt attgaactgc atgttctcaa aagcaacttc tattaccgct accatgatga
    80341 tggcagcgat gtgacggcaa caacagagta tcagggcgag atggtcgatt attcgcgtca
    80401 cgccgtcctt ctcggcagta gttgaatggc ggagctacgc tttattcgta cccacggcag
    80461 ccgttttact ccccaggatt gcacactgtt taactggctg gcacgcatta tcaccccggt
    80521 tctgcaatca tggctcaatg atgaagaaca gcaggtggcg ctgcgtttgc tggagaaaga
    80581 tcgcgatcat catcgggtac tggtggatat cactaatgca gtgctgtcac atcttgatct
    80641 cgacgatctg atcgctgacg tcgctcgtga gatccatcat tttttcggtc tggcttcagt
    80701 cagtatggta ctgggcgatc atcgaaagaa cgagaagttc agcctgtggt gcagcgatct
    80761 ttctgcctca cattgtgcgt gtctgccaca caatatgcct ggcgacagtg tattgctgac
    80821 acaaacgcta caaacccgac aaccgacctt gacgcaccgt gcagatgatc tgtttctctg
    80881 gcaacgcgac ccgttattac tcttacttgc atctaacggc tgcgaatctg cgctccttat
    80941 accgcttacc tttggcaacc atacaccggg tgcattgttg ctggcgcata cctcttccac
    81001 tctctttagt gaggaaaact gccagctact acaacacata gccgatcgca tcgctattgc
    81061 cgttggcaat gccgatgcct gccgtagaat gaccgatttg caggaaagtt tgcagcaaga
    81121 aaaccaccag cttagcgagc agctcctttc gaatctgggc gtcggtgaca ttatctatca
    81181 aagccaggca atggaagacc tgctccagca ggtagatatt gtggcgaaga gcgacagtac
    81241 ggtgttgatt tgcggtgaaa ccggaaccgg caaagaggtg atcgccagag cgatccatca
    81301 acttagcccg cgacacgaca agccgctggt caaaatcaac tgcgctgcca tccccgccag
    81361 tcttctggaa agtgagttat tcggtcatga caaaggggcg tttactggtg cgattaatac
    81421 ccatcgtggt cgttttgaaa ttgccgatgg cggcacgttg tttctcgatg aaattggcga
    81481 tctgccgtta gaacttcagc ctaaactgct gcgcgtattg caggaacggg agattgagcg
    81541 tctcggcggg agtagaacga tcccggtaaa tgtcagagtc attgccgcca ccaaccgtga
    81601 tttgtggcaa atggttgaag atcgccagtt tcgcagcgat ctcttttatc gcctgaatgt
    81661 cttcccactg gaattgcctc cgctgcgcga ccgtccggaa gatatccctc ttttagcaaa
    81721 gcatttcacg caaaaaatgg cgcgccatat gaatcgttca attgacgcca tcccgaccga
    81781 ggcgctacgc cagttgatgt cgtgggactg gccgggcaac gtgcgcgagc tggaaaacgt
    81841 gattgagcga gcggtactgc tgactcgcgg taacagtctg aatttacatc ttaatgtccg
    81901 acaaagccgt ttactgccga cgctaaatga agattcagcg cttcgcagtt caatggcgca
    81961 gttgctgcac ccgacgacgc cagagaatga cgaagaagaa cgtcagcgca ttgttcaggt
    82021 attgcgagaa accaatggca ttgttgccgg gccccgtggc gcggcgacac gattagggat
    82081 gaagcgcacc acgctgctgt cacgaatgca gcgtctggga atctcggttc gcgaggtgtt
    82141 gtaatctgct tttgcaggag tatgcatgag aaacaaactc tctttcgact tgcagttgag
    82201 cgccagaaaa gcggcaatcg ctgaacggat tgccgcccat aaaattgccc gcagtaaagt
    82261 gtcggtcttt ttaatggcga tgtccgctgg cgtgtttatg gcgatcggat ttacttttta
    82321 cctttccgtt atcgccgatg ccccgtcttc ataggcatta acccatctgg tgggcggcct
    82381 ttgctttaca cttggcttta ttttgctggc ggtttgcggc accagcctgt tcacctcgtc
    82441 ggtaatgacg gtaatggcaa aaagtcgggg cgttattagt tggcgaactt ggctgattaa
    82501 cgcacttctg gtggcctgcg gtaatctggc aggtattgcc tgtttcagtt tgttaatctg
    82561 gttttccggg ctggtgatga gtgaaaacgc gatgtgggga gtcgcgattt tacactgcgc
    82621 cgagggcaaa atgcatcata catttactga atctgtcagc ctcggcatta tgtgcaatct
    82681 gatggtttgc ctggcgctgt ggatgagtta ttgcggacgt tcgttatgcg acaaaatcgt
    82741 cgccatgatt ttgtccatca ccctgtttgt cgccagtggc tttgagcact gtatcgccaa
    82801 tttgtttgtg attccgttcg ccattgccat tcgccatttc gcccctacct ccttctggca
    82861 actggcgcac agtagcgcag acaattttcc ggcactgacg gtcagccatt ttattaccgc
    82921 caatctgctc ccggtgatgc tgggtaatat tatcggcggt gcagtgctgg tgagtatttg
    82981 ttatcgggct atttatttac gtcaggaatc ctgaaaaatc agcccggcga aacagttcgt
    83041 cgggctgaag attattcttg cgcgatttgc ccatcgggcc aggcgtggat cacggctttg
    83101 atcagcgtcg ccaatggaat ggcgaagaat acgccccaga atccccacaa accaccgaag
    83161 atcaccaccg ataaaataat caccagcgga tgcaggttaa ccgcttcgga gaacaacacc
    83221 ggtactagca ggttgccgtc cagcgcctga ataatcagat acaccgcgaa gcagctccag
    83281 aattcagtac ctgcgccaaa ctggaatagc gccacgccaa ccaccggaat ggtcaccaca
    83341 aatgcgccaa tgtacggaat aagaaccgag aagccgacca gcactgccag cagcagcgaa
    83401 tagttcagcc cgaagagcaa gaaccccagc caggtggcga tccccaccac gatcatctcc
    83461 agcactttgc cgcggatata gttggtgatt tgttgattca tctccttcca cacctgtcct
    83521 gccagcccac ggttacgcgg cagcacccgg cgaacggcgt tcagcatctg ctctttgtct
    83581 ttcagcagga agaagaccat caatggcacc agcaccagat agacggctat ggtcagcaaa
    83641 ccgaccagcg aggcgaggga aattttcacc accgaatcgc ccatggtcag catccgactg
    83701 cgcatatttt cggccattgc atcaataatg cccgcgtcca ttaacaccgg atagcggcgc
    83761 ggcaacgtgg cggcaaagtc agaaagctta ttgagcatcc ccggcatatc gcggattaag
    83821 tagatgccct gttgccaggc gataggcagt accacgaacg ccatcagtag caatataccg
    83881 acaaaaacca ccaatacaat cgacgtcgcc cagcggcggg agcagccaat agattgcagg
    83941 cgcacggttg gccattccag caaataagcc agcacaatag ccaccagcaa cggagcaagc
    84001 aggccactaa agaaaaagat aatgccaaat ccggcaacta aaataaccag caaggcaatc
    84061 gcttccgggt cgctaaaacg gcggcgatac cattgcatca acatttcgag cataaagacc
    84121 ttccctgaac ctcaagagcg ggattgcgat ccgcaattgt atcgaaatgt cacaaaaaag
    84181 acttcgcttt ttatgacgga ttcaggaaac tgaaaagtca tttgagtggg ctaatcttcg
    84241 ccgttacact caaaggcggc gaggtgggaa cgatatttca cagtatcggt caaatgacta
    84301 tcttcagaaa tacaggatag aggttatgtt caggcagttg aaaaaaaacc tggttgcaac
    84361 cctcattgct gctatgacca ttggtcaggt agccccggcg tttgccgaca gcgcagacac
    84421 cttgccggat atgggaacct ccgcaggaag cacgctttcc attggtcagg aaatgcagat
    84481 gggcgactat tatgtccgcc agctacgcgg cagcgcgccg ttaattaatg acccgctgtt
    84541 aacgcaatat attaattcgc tggggatgcg tctggtttcg catgccaatt cggttaagac
    84601 accgtttcat ttttttctga tcaacaacga cgaaattaac gcctttgctt tctttggcgg
    84661 caacgtggtg ctgcactctg ccctgttccg ttattccgat aacgaaagtc aactggcttc
    84721 agttatggcg cacgaaatct cccacgtcac ccaacgtcac ctggcgcgag cgatggaaga
    84781 tcaacaacgc agcgcgccgc tgacctgggt cggcgcgtta ggttctattt tactggcgat
    84841 ggccagtccg caggcgggga tggcggcgct gaccggtaca ctggcgggaa cgcgccaggg
    84901 gatgatcagt ttcacccagc aaaatgaaca ggaagcggac cgcattggta ttcaggtgct
    84961 gcaacgctcg ggattcgatc cacaggcgat gccgaccttc ctggaaaaat tgctcgatca
    85021 ggcgcgttac tcctcgcgcc cgccggaaat tttattgact cacccgttgc cggaaagtcg
    85081 tctggcagat gcccgcaacc gtgctaatca gatgcgcccg atggtggtgc agtcgtcgga
    85141 agatttctat ctggcgaaag cgcgcacact ggggatgtat aattccggac gtaaccagct
    85201 caccagtgat ttgctggatg aatgggcgaa aggaaacgtt cttcagcaac gagcggcgca
    85261 atatggtcgt gctttacagg cgatggaagc caataaatac gacgaggcgc gtaaaacgct
    85321 gcaaccgtta ctggcggcag aacctggcaa cgcatggtat ctcgatctgg ctaccgatat
    85381 cgatcttgag caaaacaaag ccaatgaggc aatcaatcgc ctgaaaaatg cccgcgattt
    85441 acgcaccaat ccggtgttgc agctcaacct ggcgaacgct tatctgcaag gcggtcaacc
    85501 acaagaagcg gccaatattc tgaatcgcta cacctttaat aataaagatg acagcaacgg
    85561 ctgggatttg ctggcacagg cggaagccgc gctaaataac cgcgatcagg agctggctgc
    85621 gcgagcagaa ggttatgcgc tcgccggacg actcgatcag gccatttcgc tgttgagtag
    85681 cgccagttcg caggtgaaat taggcagcct gcaacaagcg cgttacgatg cgcgcatcga
    85741 ccagttgcgc cagctgcagg aacgctttaa gccttatacc aagatgtaat tcgcattaaa
    85801 ggaagaacaa tgaccaaaca ggtaaaaatc taccataacc cacgctgttc aaagagccgg
    85861 gaaacgctga acctgctgaa agaaaacggc gtggagccag aagtggtgct ctaccttgag
    85921 acacccgccg atgcggcaac gctgcgcgat ttgctgaaaa tgctggggat gaatagcgcc
    85981 cgtgaattga tgcgccagaa agaggatctt tataaagaac taaacctggc tgacagttcg
    86041 cttagcgaag aggcgctgat tcaggcaatg gtggataatc cgaagctgat ggagcgcccg
    86101 attatggtcg cgaatggcaa agcgcggatt ggtcgtccac cggagcaggt actggaaatc
    86161 gtgggttaag atgtgctcca tcgctagttg aagcacattg ccggatgcga cgcttgccac
    86221 gtcttatccg gcctacgaat actatggatt aacttcgcgt aggtcaggcg ttcgcgccgc
    86281 atccgacaat aaacacctta tctacaactt cagaatttct ttcacaaacg gaatggtcag
    86341 cttacgttgc gcggtaatcg acgcacgatc caactgatcc aacgtcataa atagcgtgcg
    86401 catttctctg tcgagccgct tcagcaagaa acgccccaca tcttccggca gttcaaaacc
    86461 acgcaaacgc gcgcgtaact gtagcgcctg caacttatct tcatcagaaa gtggctgcaa
    86521 tttgtagatc tgcccccagt cgagacgcga cgcgagatcc ggtaatccca gattcaactg
    86581 ccgcggtgga cgatcgccgg tgatcaacaa ccgtgttttg cccgattcca gaattcgatt
    86641 gtagagatcg aaaatcgcca tctcccacaa ctcatcgcct gcaatacact caatgttgtc
    86701 gatacagacc agcgacaaat gctccatacc gtcgagcact tccggaacaa accaggtgcg
    86761 tttatccagc gggacatagc ccaccgcatc gccacgctgc gacaattccg cgcaagccgc
    86821 gtgcagcaga tggctgcgcc ccgcgccttc gcgtgcccag agatagatgt aaccgctatg
    86881 ttcctgacgc agcacgtttt gcagcgcggc cagtaaagag gagttatccc ccggccagaa
    86941 acttgcaaag gtttcgtcgt caggaagata aagtggcaaa gagagctgtg ccggtgtgtt
    87001 cagagatacc tcaaccagga tttcacaaaa tcgcgagaag tttaccacag aatcccatga
    87061 tgtttgaacc gggcagcaac actgcccggt cggtacatta tttgtctgtt atatccgcgt
    87121 cttctgcgtc cagcaccact tcttcagggc gcagcacact gatcagtttg aaaatcaaac
    87181 ttaagccgat accgacgatg gtcgccagcg ccataccttt taactccgcc gcgccgatgt
    87241 ttaccttcgc gccactgacg ccgatgatca aaatcacgga agtcaggatc aggttctgtg
    87301 ctttgttgta atccactttc gattcgatca aaacacgaat accggaagca ccaatgacac
    87361 cataaagcag cagcgaaacg ccgcccatca ccggcaatgg gatcatctgg atagcggcag
    87421 ccagtttacc gacgcaggaa agcaggatag cgaaaatcgc cgccccgccg ataacccagg
    87481 tactgtaaac acgggtgatc gccatcacgc caatgttttc tccgtaagtg gtatttggcg
    87541 tagagccaaa gaagccggag atcacggtcg acaggccgtt agcaaacatc gaacggtgca
    87601 gacctggatc gcgcagcaga tcttttttga cgatattagc cgttactacc aggtgcccta
    87661 cgtgttcggc aataaccact aacgccgctg gcagaatagt cagaatggca aaccactcga
    87721 agcgcggcgt atagagggtt ggcagtgcaa accagtgagc attaataatc ggcgtggtat
    87781 cgacaattcc cattgcgaaa gagagcgcgt accccaccag cacgccaatt aaaatcggga
    87841 taattgccag gaaaccacga aacagcacgg aacctaaaac cgtgaccgcc agggtggtaa
    87901 tagagatgat gatggttttg gagtttggcg tttgcccttc agccgggagt aaacccgcca
    87961 taccggcagc tacgcccgcc agctccagac cgatgacggc aacgattgcg cccattgccg
    88021 caggtggaaa cagcacgtcc aaccagccgg tccccgcttt cttcacgata aaagaaacca
    88081 ggcagaacag cacgccgcac ataataaagc cgcccagcgc gacttcatac cctaacggca
    88141 acagtaacaa taccggtgaa ataaaggcaa agctggaacc aagataagcc ggaattttcc
    88201 ctttacagat gaagagatac agcagcgttc caataccgtt aaataacagt acagtcgccg
    88261 ggttaatatg aaataagacg ggcaccagga cggttgcacc aaacatggcg aacaaatgtt
    88321 gcaaactaag cgggattgtc tgtaaaagtg gcggtctttc actcaccccg atagcacggc
    88381 gcgtcatagt attatcctct gtattatgtg ttataggcgc tttactcaaa aaaaagccga
    88441 ctcttaaagt cggctttaat tatttttatt ctttatttcg taccaaagat tttgtcaccg
    88501 gcatcgccga ggcccggaat aatgtatccg tgctcgttca gtccctggtc aatcgatgca
    88561 gtatacagtt cgacgtccgg gtgcgctttt tccagcgcag cgataccttc tggcgcagcg
    88621 accagcacca gaactttgat gctgctgcag cccgcttttt tcagcaggtc gatggtcgcg
    88681 ataacggaac caccggttgc cagcattggg tcaacgatca gcgccatacg ctcatcgatg
    88741 ttagaaacca gtttctggaa gtacggtacc ggctccagcg tttcttcatt acggtacata
    88801 ccgacaacgc tgatgcgcgc gctcggaacg ttttccagca caccgtccat cataccaaga
    88861 cccgcacgca gaattggcac aacggtaatt ttcttacctt tgatctggtc gatttctacc
    88921 gggccgttcc agccttcgat agttactttt tccgtttcga ggtcggcggt cgcttcgtaa
    88981 gtcagcaggc tacccacttc ggaagcgagt tcgcgaaagc gcttggtgct gatatcttgc
    89041 tcacgcatca gtcccagctt gtgtttgacg agtgggtgtt tgacttccac gatcttcata
    89101 ctctttctcc tttgaggggc agccacaaaa aaaaatcgac ggattatacc tcctttcttc
    89161 aaggcggcaa tattattttc gttgacttta gtcaaaatga taacagtttg agataaagtt
    89221 attttatatt caaatggtta tgaaagaaga ttattccatc cgaaaactaa cctttgccct
    89281 ggcacaagtc ttctttcgcc gcgcgcccgg ggaaaagacg tgcaaaaagg ttgtgtaaaa
    89341 cagtctcgca aacgtttgct ttccctgtta gaattgcgcc gaattttatt tttctaccgc
    89401 aagtaacgcg tggggaccca agcagtgacc gataaaacct ctcttagcta caaagatgcc
    89461 ggtgttgata ttgacgcggg taatgctctg gttggaagaa tcaaaggcgt agtgaagaaa
    89521 acgcgtcgtc cggaagtgat gggcggtctg ggcggcttcg gtgcgctgtg tgcattgccg
    89581 caaaaatatc gtgaacccgt gctggtttcc ggcactgacg gcgtaggtac caagctgcgt
    89641 ctggcaatgg acttaaaacg tcacgacacc attggtattg atctggtcgc catgtgcgtt
    89701 aatgacctgg tggtgcaagg tgcggaaccg ctgtttttcc tcgactatta cgcaaccgga
    89761 aaactggatg ttgataccgc ttcagcagtg atcagcggca tcgcggaagg ttgtctgcaa
    89821 tcgggctgtt ctctggtggg tggcgaaacg gcagaaatgc cggggatgta tcacggtgaa
    89881 aattacgatg tcgcgggttt ctgcgtgggc gtggtagaaa aatcagaaat catcgacggc
    89941 tctaaagtca gcgacggcga tgtgctgatt gcactcggtt ccagcggtcc gcactcgaac
    90001 ggctattcgc tggtgcgcaa aattcttgaa gtcagcggtt gtgatccgca aaccaccgaa
    90061 cttgatggta agccattagc cgatcatctg ctggcaccga cccgcattta cgtgaagtca
    90121 gtgctggagt tgattgaaaa ggtcgatgtg catgccattg cgcacctgac cggcggcggc
    90181 ttctgggaaa acattccgcg cgtattgcca gataataccc aggcagtgat tgatgaatct
    90241 tcctggcagt ggccggaagt gttcaactgg ctgcaaacgg caggtaacgt tgagcgccat
    90301 gaaatgtatc gcaccttcaa ctgcggcgtc gggatgatta tcgccctgcc tgctccggaa
    90361 gtggacaaag ccctcgccct gctaaatgcc aacggtgaaa acgcgtggaa aatcggtatc
    90421 atcaaagcct ctgattccga acaacgcgtg gttatcgaat aatgaatatt gtggtgctta
    90481 tttccggcaa cggaagtaat ttacaggcaa ttattgacgc ctgtaaaacc aacaaaatta
    90541 aaggcaccgt acgggcagtt ttcagcaata aggccgacgc gttcggcctt gaacgcgccc
    90601 gccaggcggg tattgcaacg catacgctca tcgccagcgc gtttgacagt cgtgaagcct
    90661 atgaccggga gttgattcat gaaatcgaca tgtacgcacc cgatgtggtc gtgctggctg
    90721 gttttatgcg cattctcagc ccggcgtttg tctcccacta tgccgggcgt ttgctgaaca
    90781 ttcacccttc tctgctgccg aaatatcccg gattacacac ccatcgtcag gcgctggaaa
    90841 atggcgatga agagcacggt acatcggtgc atttcgtcac cgatgaactg gacggtggcc
    90901 cggttatttt acaggcgaaa gtcccggtat ttgctggtga tacggaagat gacatcaccg
    90961 cccgcgtgca aacccaggaa cacgctattt atccactggt gattagctgg tttgccgatg
    91021 gtcgtctgaa aatgcacgaa aacgccgcgt ggctggatgg tcaacgtctg ccgccgcaga
    91081 gctacgctgc cgacgagtaa tgcccccgta gttaaagcgc cagctctgcc gctggcgttt
    91141 ttcaattcac ctgtaaatcg caagctccag cagttttttt cccccttttc tggcatagtt
    91201 ggacatctgc caatattgct cgccataata tccaggcagt gtcccgtgaa taaaacggag
    91261 taaaagtggt aatgggtcag gaaaagctat acatcgaaaa agagctcagt tggttatcgt
    91321 tcaatgaacg cgtgcttcag gaagcggcgg acaaatctaa cccgctgatt gaaaggatgc
    91381 gtttcctggg gatctattcc aataaccttg atgagttcta taaagtccgc ttcgctgaac
    91441 tgaagcgacg catcattatt agcgaagaac aaggctccaa cgctcattcc cgccatttac
    91501 tgggcaaaat tcagtcccgg gtgctgaaag ccgatcagga attcgacggc ctctacaacg
    91561 agctattgct ggagatggcg cgcaaccaga tcttcctgat taatgaacgc cagctctccg
    91621 tcaatcaaca aaactggctg cgtcattatt ttaagcagta tctgcgtcag cacattacgc
    91681 cgattttaat caatcctgac actgacttag tgcagttcct gaaagatgat tacacctatc
    91741 tggcggtgga aattatccgt agcgatagca tccgttacgc gctgctggag atcccatcag
    91801 ataaagtgcc gcgctttgtg aatttaccgc cagaagcgcc gcgtcgacgc aagccgatga
    91861 ttcttctgga taacattctg cgttactgcc ttgatgatat tttcaaaggc ttctttgatt
    91921 atgacgcgct gaatgcctat tcaatgaaga tgacccgcga tgccgaatac gatttagtgc
    91981 atgagatgga agccagcctg atggagttga tgtcttccag tctcaagcag cgtttaactg
    92041 ctgagccggt gcgttttgtt tatcagcgcg atatgcccaa tgcgctggtt gaagtgttac
    92101 gcgaaaaact gactatttcc cgctacgact ccatcgtccc cggcggtcgt tatcataatt
    92161 ttaaagactt tattaatttt cccaatgtcg gcaaagccaa tctggtgaac aaaccactgc
    92221 cgcgtttacg ccatatttgg tttgataaag cccagttccg caatggtttt gatgccattc
    92281 gcgaacgcga tgtgttgctc tattatcctt atcacacctt tgggcatgtg ctggaactgc
    92341 tgcgtcaggc ttcgttcgac ccgagcgtac tggcgattaa aattaacatt taccgcgtgg
    92401 cgaaagattc acgcatcatc gactcgatga tccacgccgc acataacggt aagaaagtca
    92461 ctgtggtggt tgagttacag gcgcgtttcg acgaagaagc caacattcac tgggcgaagc
    92521 gcctgaccga agcaggcgtg cacgttatct tctctgcgcc ggggctgaaa attcacgcca
    92581 aactgttcct gatttcacgt aaagaaaacg gtgaagtggt ccgttacgca cacatcggga
    92641 ccgggaactt taacgaaaaa accgcgcgtc tttatactga ctattcgttg ctgaccgcag
    92701 atgcgcgtat caccaacgaa gtacggcggg tatttaactt tattgaaaac ccataccgcc
    92761 cggtgacatt tgattattta atggtgtcac cgcaaaactc tcgccgtctg ttatatgaaa
    92821 tggtagaccg cgaaatcgcc aacgcgcagc aagggctgcc cagtggtatc accctgaagc
    92881 taaataacct tgtcgataaa ggcctggttg atcgtctgta tgcggcctcc agctccggcg
    92941 taccggttaa tctgctggtt cgcggaatgt gttcgctgat ccccaatctg gaaggcatta
    93001 gcgacaacat tcgtgccatc agtattgttg accgttacct tgaacatgac cgggtttata
    93061 tttttgaaaa tggcggcgat aaaaaggtct acctttcttc cgccgactgg atgacgcgca
    93121 atattgatta tcgtattgaa gtggcgacac cgctgctcga tccgcgcctg aagcagcggg
    93181 tgctggacat catcgacata ttgttcagcg atacggtcaa agcacgttat atcgataaag
    93241 aactcagtaa tcgctacgtt ccccgcggca atcgccgcaa agtacgggcg cagttggcga
    93301 tttacgacta catcaaatca ctcgaacaac ctgaataacc ctatgccaat acacgataaa
    93361 tcccctcgtc cgcaggagtt tgctgcggtc gatcttggtt caaacagttt tcacatggtc
    93421 atagcccgtg tggtagatgg tgccatgcag attattggcc gcctgaaaca gcgggtgcat
    93481 ctggcggacg gcctggggcc agataatatg ttgagtgaag aggcaatgac gcgcggtttg
    93541 aactgtctgt cgctgtttgc cgaacggcta caagggtttt ctcctgccag cgtctgtata
    93601 gtcggcaccc atacgctgcg tcaggcgctg aacgccactg actttctgaa acgcgcggaa
    93661 aaggtcattc cctacccgat tgaaattatt tccggtaatg aagaagcccg tctgattttt
    93721 atgggcgtgg aacataccca accggaaaaa ggtcgcaaac tggttattga tattggcggc
    93781 ggatctacgg aactggtgat tggtgaaaat ttcgaaccta ttctcgttga aagccgccgg
    93841 atgggttgtg tcagctttgc ccagctttat tttcctggcg gggtcatcaa taaagagaat
    93901 tttcagcgcg ctcgcatggc ggcagcacaa aaactggaaa ctttaacctg gcaattccgt
    93961 attcagggct ggaacgttgc aatgggcgct tccggtacca taaaagccgc ccatgaagtg
    94021 ttaatggaaa tgggcgagaa agacgggata attaccccgg aacgtctgga aaaactggta
    94081 aaagaaattt tacgtcaccg taatttcgca tcgctgagtt taccgggtct ttccgaagag
    94141 cggaaaacag tcttcgttcc tggactggcg attttatgtg gtgtgtttga tgctttagcc
    94201 atccgtgaac tgcgcctttc tgacggggcg ttacgcgaag gcgtactgta tgaaatggaa
    94261 ggacgtttcc gtcatcagga tgtgcgtagt cgcaccgcca gcagcctcgc caaccagtat
    94321 cacatagaca gcgaacaggc ccgacgagtg ctggatacca ctatgcaaat gtacgaacag
    94381 tggcgggaac agcaaccgaa gctggcgcat ccgcaactgg aggcgctact gcgatgggcc
    94441 gccatgctgc atgaggtcgg gttgaatatc aaccacagcg gtttgcatcg ccactccgct
    94501 tatattctgc aaaacagtga cttgccgggt tttaatcagg aacagcagct gatgatggcg
    94561 acactgctgc gctatcaccg taaagcgatt aagctcgacg atctgccgcg ctttaccttg
    94621 tttaagaaga aacagttcct gccactgata cagctattgc gccttggcgt attactaaac
    94681 aatcaacgtc aggcaaccac cacaccgcca acattgacac tgattactga tgacagtcac
    94741 tggacactgc gtttcccgca tgactggttt agtcagaatg cgctggtact gcttgatctg
    94801 gaaaaggagc aagaatactg ggaaggcgtg gctggctggc ggttgaaaat tgaagaagaa
    94861 agtaccccag aaatcgccgc ttaatgcttt gcgggccgac gagaaatgtc ggcccgcatt
    94921 attcaggcac tttcgcgaat gggttcgatt tcattcagcg tatcaattaa cggttgcggc
    94981 ttaccaataa gataaccctg catataatcg atccccaaag agagcaccgc ctcgcggatc
    95041 tcttcgtttt caacgtactc tgccactacc cgcattttct tcattcgcgc cagatggcaa
    95101 atcgacgcca caatctgata atccagacta tttgacacaa tattgcggat aaagctgccg
    95161 tcaattttaa gcagatcggc attcacattt ttaagccgtg catagctggc gtagccggtg
    95221 ccgaaatcat caatcgcaat ctggcagcct aattcctgaa gatgctgcaa ggtgatttgc
    95281 gcctgcttaa cattggtcag agcattactt tcggtgactt caaaaataag ttgccacgct
    95341 tcaatctgat atttagccag caactgactg acttcaacag gaaaacgagc ctgacatacc
    95401 gaggttggag acagattaat agcaaaacgg tgagcgggca tcttcgctct gttttcagcc
    95461 ataaattgca gcgtatgctc aatgacccac atgtcgatac tcgacgataa accaaattcg
    95521 tgcgcgaccg gcaagaagct atcggggccg atcagttcat cattctcacc tttcatgcgc
    95581 agaagaattt catggtaaac atcaccacgc ataccggtaa tcggctgggc catcaggaaa
    95641 aaatggttgt gctccagcgc ctgctgtagt cgattcatca tcgcgacttt atctttcaat
    95701 tcgcgttgca aatacattgc cccgcaacgc tgcatatttt ccggggcgtt ggtcacgatg
    95761 gaaagttcgg cgaccgtatt tagctctccc agcagcaggt agatatgatt cactggcgag
    95821 cgcacatagc agtaactgac gccaatctgc ggttgcatcg gcatcccatc ccaaaagaaa
    95881 cgaaattgct tgagatgact atccagtgcg gtaatgcgct cctggtgcga ttctgtattc
    95941 agtcgcagcg cgagatcgtt acccgaaagc tgataaacat cttcacccgg ttccagcaag
    96001 ggtgacagcc agtgagaaag tttttgcttg t
//
LOCUS       DADMEQ010000002        85595 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.002, whole genome shotgun sequence.
ACCESSION   DADMEQ010000002 DADMEQ010000000
VERSION     DADMEQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 85595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.002"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..1592)
                     /locus_tag="JNZ05_000092"
     CDS             complement(<1..1592)
                     /locus_tag="JNZ05_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY8563464.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYMFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQA"
     gene            complement(1576..1932)
                     /locus_tag="JNZ05_000093"
                     /pseudo
     CDS             complement(1576..1932)
                     /locus_tag="JNZ05_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            complement(2055..2237)
                     /locus_tag="JNZ05_000094"
     CDS             complement(2055..2237)
                     /locus_tag="JNZ05_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8563465.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(2221..2661)
                     /locus_tag="JNZ05_000095"
     CDS             complement(2221..2661)
                     /locus_tag="JNZ05_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY8563466.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(2661..2957)
                     /locus_tag="JNZ05_000096"
     CDS             complement(2661..2957)
                     /locus_tag="JNZ05_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY8563467.1"
                     /translation="MTEELITLEEVKLHRRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            complement(2954..3292)
                     /locus_tag="JNZ05_000097"
     CDS             complement(2954..3292)
                     /locus_tag="JNZ05_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY8563468.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(3289..4500)
                     /locus_tag="JNZ05_000098"
     CDS             complement(3289..4500)
                     /locus_tag="JNZ05_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY8563469.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLSAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(4502..5074)
                     /locus_tag="JNZ05_000099"
     CDS             complement(4502..5074)
                     /locus_tag="JNZ05_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAY8563470.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFSFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPREDNRRWWADLAGV"
     gene            complement(5114..6271)
                     /locus_tag="JNZ05_000100"
     CDS             complement(5114..6271)
                     /locus_tag="JNZ05_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY8563471.1"
                     /translation="MQRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGNTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPEDDD"
     gene            complement(6574..6735)
                     /locus_tag="JNZ05_000101"
     CDS             complement(6574..6735)
                     /locus_tag="JNZ05_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HAY8563472.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(6745..7050)
                     /locus_tag="JNZ05_000102"
     CDS             complement(6745..7050)
                     /locus_tag="JNZ05_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8563473.1"
                     /translation="MSEPKFGEKFYKHNERITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(7047..7325)
                     /locus_tag="JNZ05_000103"
     CDS             complement(7047..7325)
                     /locus_tag="JNZ05_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8563474.1"
                     /translation="MHTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(7517..9265)
                     /locus_tag="JNZ05_000104"
     CDS             complement(7517..9265)
                     /locus_tag="JNZ05_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY8563475.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRPQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(9262..9561)
                     /locus_tag="JNZ05_000105"
     CDS             complement(9262..9561)
                     /locus_tag="JNZ05_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAY8563476.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(9567..9800)
                     /locus_tag="JNZ05_000106"
     CDS             complement(9567..9800)
                     /locus_tag="JNZ05_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8563477.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(9802..10022)
                     /locus_tag="JNZ05_000107"
                     /pseudo
     CDS             complement(9802..10022)
                     /locus_tag="JNZ05_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10015..10236)
                     /locus_tag="JNZ05_000108"
     CDS             complement(10015..10236)
                     /locus_tag="JNZ05_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8563478.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(10226..10426)
                     /locus_tag="JNZ05_000109"
     CDS             complement(10226..10426)
                     /locus_tag="JNZ05_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HAY8563479.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSTGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            complement(10435..11181)
                     /locus_tag="JNZ05_000110"
     CDS             complement(10435..11181)
                     /locus_tag="JNZ05_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8563480.1"
                     /translation="MKIEYTPERGRGFVRPGETGKPQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCVCDPKHKHGDDSRYQAGGQCNQSGSVRCHNCEVLSFDEYSLRYFSRAKEHGA
                     NRSDSCSFFLRRLFRAGDVFSVDSSLVIVCACKAMRRSTSHHGADSFYPLGSMPRCCN
                     SLRIIRFIQAARESSPSCCCAFSIFSRKSGSKRNWNGGLPRLSFLCVDTLITPHVMYL
                     CVMTHYTQSTQKAKPRTVGAVTGLLTNNVN"
     gene            complement(11239..11427)
                     /locus_tag="JNZ05_000111"
     CDS             complement(11239..11427)
                     /locus_tag="JNZ05_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY8563481.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(11802..13031)
                     /locus_tag="JNZ05_000112"
     CDS             complement(11802..13031)
                     /locus_tag="JNZ05_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY8563482.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            13280..14401
                     /locus_tag="JNZ05_000113"
     CDS             13280..14401
                     /locus_tag="JNZ05_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAY8563483.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(14450..15676)
                     /gene="pepT"
                     /locus_tag="JNZ05_000114"
     CDS             complement(14450..15676)
                     /gene="pepT"
                     /locus_tag="JNZ05_000114"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAY8563484.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVAEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTNGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            15926..17062
                     /gene="potA"
                     /locus_tag="JNZ05_000115"
     CDS             15926..17062
                     /gene="potA"
                     /locus_tag="JNZ05_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HAY8563485.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            17046..17903
                     /gene="potB"
                     /locus_tag="JNZ05_000116"
     CDS             17046..17903
                     /gene="potB"
                     /locus_tag="JNZ05_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HAY8563486.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            17900..18694
                     /gene="potC"
                     /locus_tag="JNZ05_000117"
     CDS             17900..18694
                     /gene="potC"
                     /locus_tag="JNZ05_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HAY8563487.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            18691..19737
                     /gene="potD"
                     /locus_tag="JNZ05_000118"
     CDS             18691..19737
                     /gene="potD"
                     /locus_tag="JNZ05_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HAY8563488.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSQLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(19893..20714)
                     /gene="cobB"
                     /locus_tag="JNZ05_000119"
     CDS             complement(19893..20714)
                     /gene="cobB"
                     /locus_tag="JNZ05_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAY8563489.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(20730..21641)
                     /gene="nagK"
                     /locus_tag="JNZ05_000120"
     CDS             complement(20730..21641)
                     /gene="nagK"
                     /locus_tag="JNZ05_000120"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HAY8563490.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWEDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(21670..22914)
                     /gene="lolE"
                     /locus_tag="JNZ05_000121"
     CDS             complement(21670..22914)
                     /gene="lolE"
                     /locus_tag="JNZ05_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HAY8563491.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(22914..23615)
                     /gene="lolD"
                     /locus_tag="JNZ05_000122"
     CDS             complement(22914..23615)
                     /gene="lolD"
                     /locus_tag="JNZ05_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HAY8563492.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(23608..24807)
                     /gene="lolC"
                     /locus_tag="JNZ05_000123"
     CDS             complement(23608..24807)
                     /gene="lolC"
                     /locus_tag="JNZ05_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HAY8563493.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            25069..26115
                     /locus_tag="JNZ05_000124"
     CDS             25069..26115
                     /locus_tag="JNZ05_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY8563494.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIFSRLALPLFA
                     LFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLRRHLLMASLL
                     TVLAVGAWLFTISLLLSLVSTVVIMKLFYQYEQRFGMRSTSLLNVIGSNTIAIYTTHR
                     ILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKLSQRAFSDLL
                     FSPPSLPAAVSYSR"
     gene            26243..29689
                     /gene="mfd"
                     /locus_tag="JNZ05_000125"
     CDS             26243..29689
                     /gene="mfd"
                     /locus_tag="JNZ05_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAY8563495.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRYVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     SERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLNDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGMIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            29832..30794
                     /gene="ldtC"
                     /locus_tag="JNZ05_000126"
                     /pseudo
     CDS             29832..30794
                     /gene="ldtC"
                     /locus_tag="JNZ05_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(30877..31134)
                     /gene="bhsA"
                     /locus_tag="JNZ05_000127"
     CDS             complement(30877..31134)
                     /gene="bhsA"
                     /locus_tag="JNZ05_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HAY8563496.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            31375..32007
                     /gene="comR"
                     /locus_tag="JNZ05_000128"
     CDS             31375..32007
                     /gene="comR"
                     /locus_tag="JNZ05_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HAY8563497.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(32069..32608)
                     /locus_tag="JNZ05_000129"
     CDS             complement(32069..32608)
                     /locus_tag="JNZ05_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY8563498.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(32835..34139)
                     /locus_tag="JNZ05_000130"
     CDS             complement(32835..34139)
                     /locus_tag="JNZ05_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY8563499.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(34540..35082)
                     /locus_tag="JNZ05_000131"
     CDS             complement(34540..35082)
                     /locus_tag="JNZ05_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY8563500.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(35105..36130)
                     /gene="nagZ"
                     /locus_tag="JNZ05_000132"
     CDS             complement(35105..36130)
                     /gene="nagZ"
                     /locus_tag="JNZ05_000132"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAY8563501.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(36141..36965)
                     /gene="thiK"
                     /locus_tag="JNZ05_000133"
     CDS             complement(36141..36965)
                     /gene="thiK"
                     /locus_tag="JNZ05_000133"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HAY8563502.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(36946..37587)
                     /gene="lpoB"
                     /locus_tag="JNZ05_000134"
     CDS             complement(36946..37587)
                     /gene="lpoB"
                     /locus_tag="JNZ05_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HAY8563503.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(37602..37979)
                     /locus_tag="JNZ05_000135"
     CDS             complement(37602..37979)
                     /locus_tag="JNZ05_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HAY8563504.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(37982..38341)
                     /gene="hinT"
                     /locus_tag="JNZ05_000136"
     CDS             complement(37982..38341)
                     /gene="hinT"
                     /locus_tag="JNZ05_000136"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HAY8563505.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            38675..40864
                     /gene="fhuE"
                     /locus_tag="JNZ05_000137"
                     /pseudo
     CDS             38675..40864
                     /gene="fhuE"
                     /locus_tag="JNZ05_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(40924..42357)
                     /gene="ptsG"
                     /locus_tag="JNZ05_000138"
     CDS             complement(40924..42357)
                     /gene="ptsG"
                     /locus_tag="JNZ05_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAY8563506.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(42652..43449)
                     /locus_tag="JNZ05_000139"
     CDS             complement(42652..43449)
                     /locus_tag="JNZ05_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY8563507.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLCDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(43460..44464)
                     /gene="holB"
                     /locus_tag="JNZ05_000140"
     CDS             complement(43460..44464)
                     /gene="holB"
                     /locus_tag="JNZ05_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAY8563508.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWVATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(44461..45102)
                     /locus_tag="JNZ05_000141"
     CDS             complement(44461..45102)
                     /locus_tag="JNZ05_000141"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAY8563509.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARTRGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(45092..46114)
                     /gene="yceG"
                     /locus_tag="JNZ05_000142"
     CDS             complement(45092..46114)
                     /gene="yceG"
                     /locus_tag="JNZ05_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HAY8563510.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(46117..46926)
                     /gene="pabC"
                     /locus_tag="JNZ05_000143"
     CDS             complement(46117..46926)
                     /gene="pabC"
                     /locus_tag="JNZ05_000143"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAY8563511.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(47046..48287)
                     /gene="fabF"
                     /locus_tag="JNZ05_000144"
     CDS             complement(47046..48287)
                     /gene="fabF"
                     /locus_tag="JNZ05_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAY8563512.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(48374..48610)
                     /gene="acpP"
                     /locus_tag="JNZ05_000145"
     CDS             complement(48374..48610)
                     /gene="acpP"
                     /locus_tag="JNZ05_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY8563513.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(48821..49555)
                     /gene="fabG"
                     /locus_tag="JNZ05_000146"
     CDS             complement(48821..49555)
                     /gene="fabG"
                     /locus_tag="JNZ05_000146"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HAY8563514.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(49568..50497)
                     /gene="fabD"
                     /locus_tag="JNZ05_000147"
     CDS             complement(49568..50497)
                     /gene="fabD"
                     /locus_tag="JNZ05_000147"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAY8563515.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(50513..51466)
                     /locus_tag="JNZ05_000148"
     CDS             complement(50513..51466)
                     /locus_tag="JNZ05_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAY8563516.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(51534..52604)
                     /gene="plsX"
                     /locus_tag="JNZ05_000149"
     CDS             complement(51534..52604)
                     /gene="plsX"
                     /locus_tag="JNZ05_000149"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAY8563517.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(52685..52858)
                     /gene="rpmF"
                     /locus_tag="JNZ05_000150"
     CDS             complement(52685..52858)
                     /gene="rpmF"
                     /locus_tag="JNZ05_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAY8563518.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(52910..53431)
                     /gene="yceD"
                     /locus_tag="JNZ05_000151"
     CDS             complement(52910..53431)
                     /gene="yceD"
                     /locus_tag="JNZ05_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HAY8563519.1"
                     /translation="MQKVKFPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            53630..54214
                     /gene="yceF"
                     /locus_tag="JNZ05_000152"
     CDS             53630..54214
                     /gene="yceF"
                     /locus_tag="JNZ05_000152"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HAY8563520.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(54326..55285)
                     /gene="rluC"
                     /locus_tag="JNZ05_000153"
     CDS             complement(54326..55285)
                     /gene="rluC"
                     /locus_tag="JNZ05_000153"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HAY8563521.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            55858..59043
                     /gene="rne"
                     /locus_tag="JNZ05_000154"
     CDS             55858..59043
                     /gene="rne"
                     /locus_tag="JNZ05_000154"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HAY8563522.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGNDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(59240..60193)
                     /gene="flgL"
                     /locus_tag="JNZ05_000155"
     CDS             complement(59240..60193)
                     /gene="flgL"
                     /locus_tag="JNZ05_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-filament junction protein FlgL"
                     /protein_id="HAY8563523.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMLTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRTELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(60205..61847)
                     /gene="flgK"
                     /locus_tag="JNZ05_000156"
                     /pseudo
     CDS             complement(60205..61847)
                     /gene="flgK"
                     /locus_tag="JNZ05_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(61913..62854)
                     /gene="flgJ"
                     /locus_tag="JNZ05_000157"
     CDS             complement(61913..62854)
                     /gene="flgJ"
                     /locus_tag="JNZ05_000157"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HAY8563524.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDQVSKTYSMNIDNLF"
     gene            complement(62854..63951)
                     /locus_tag="JNZ05_000158"
     CDS             complement(62854..63951)
                     /locus_tag="JNZ05_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HAY8563525.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAVVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(63963..64661)
                     /gene="flgH"
                     /locus_tag="JNZ05_000159"
     CDS             complement(63963..64661)
                     /gene="flgH"
                     /locus_tag="JNZ05_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HAY8563526.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(64714..65496)
                     /gene="flgG"
                     /locus_tag="JNZ05_000160"
     CDS             complement(64714..65496)
                     /gene="flgG"
                     /locus_tag="JNZ05_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HAY8563527.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(65634..66385)
                     /locus_tag="JNZ05_000161"
                     /pseudo
     CDS             complement(65634..66385)
                     /locus_tag="JNZ05_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(66405..68333)
                     /gene="flgD"
                     /locus_tag="JNZ05_000162"
                     /pseudo
     CDS             complement(66405..68333)
                     /gene="flgD"
                     /locus_tag="JNZ05_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706995.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(68345..68748)
                     /gene="flgC"
                     /locus_tag="JNZ05_000163"
                     /pseudo
     CDS             complement(68345..68748)
                     /gene="flgC"
                     /locus_tag="JNZ05_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(68752..68925)
                     /locus_tag="JNZ05_000164"
     CDS             complement(68752..68925)
                     /locus_tag="JNZ05_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgB"
                     /protein_id="HAY8563528.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVTQFADNSLQYQMSLSALSG
                     QIKGMMNVLQSGN"
     gene            69080..69739
                     /gene="flgA"
                     /locus_tag="JNZ05_000165"
     CDS             69080..69739
                     /gene="flgA"
                     /locus_tag="JNZ05_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HAY8563529.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            69815..70108
                     /gene="flgM"
                     /locus_tag="JNZ05_000166"
     CDS             69815..70108
                     /gene="flgM"
                     /locus_tag="JNZ05_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HAY8563530.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            70113..70529
                     /gene="flgN"
                     /locus_tag="JNZ05_000167"
     CDS             70113..70529
                     /gene="flgN"
                     /locus_tag="JNZ05_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HAY8563531.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDHEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(70569..72104)
                     /gene="murJ"
                     /locus_tag="JNZ05_000168"
     CDS             complement(70569..72104)
                     /gene="murJ"
                     /locus_tag="JNZ05_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAY8563532.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(72271..73137)
                     /locus_tag="JNZ05_000169"
     CDS             complement(72271..73137)
                     /locus_tag="JNZ05_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY8563533.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(73139..73786)
                     /locus_tag="JNZ05_000170"
     CDS             complement(73139..73786)
                     /locus_tag="JNZ05_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HAY8563534.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTLGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(73797..74381)
                     /gene="rimJ"
                     /locus_tag="JNZ05_000171"
     CDS             complement(73797..74381)
                     /gene="rimJ"
                     /locus_tag="JNZ05_000171"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HAY8563535.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HTCYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            74617..75825
                     /gene="mdtH"
                     /locus_tag="JNZ05_000172"
     CDS             74617..75825
                     /gene="mdtH"
                     /locus_tag="JNZ05_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HAY8563536.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSTHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            75889..76536
                     /gene="grxB"
                     /locus_tag="JNZ05_000173"
     CDS             75889..76536
                     /gene="grxB"
                     /locus_tag="JNZ05_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HAY8563537.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            76670..77230
                     /locus_tag="JNZ05_000174"
     CDS             76670..77230
                     /locus_tag="JNZ05_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY8563538.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            77336..78382
                     /gene="pyrC"
                     /locus_tag="JNZ05_000175"
     CDS             77336..78382
                     /gene="pyrC"
                     /locus_tag="JNZ05_000175"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAY8563539.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLQTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHTRHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            78456..78701
                     /gene="dinI"
                     /locus_tag="JNZ05_000176"
     CDS             78456..78701
                     /gene="dinI"
                     /locus_tag="JNZ05_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HAY8563540.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            78991..79245
                     /gene="bssS"
                     /locus_tag="JNZ05_000177"
     CDS             78991..79245
                     /gene="bssS"
                     /locus_tag="JNZ05_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HAY8563541.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            79360..80478
                     /gene="solA"
                     /locus_tag="JNZ05_000178"
     CDS             79360..80478
                     /gene="solA"
                     /locus_tag="JNZ05_000178"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HAY8563542.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            80526..80639
                     /locus_tag="JNZ05_000179"
     CDS             80526..80639
                     /locus_tag="JNZ05_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2770 domain-containing protein"
                     /protein_id="HAY8563543.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            80900..81466
                     /locus_tag="JNZ05_000180"
     CDS             80900..81466
                     /locus_tag="JNZ05_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAY8563544.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            81470..82045
                     /locus_tag="JNZ05_000181"
     CDS             81470..82045
                     /locus_tag="JNZ05_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HAY8563545.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(82087..83139)
                     /locus_tag="JNZ05_000182"
     CDS             complement(82087..83139)
                     /locus_tag="JNZ05_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAY8563546.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            83364..84284
                     /locus_tag="JNZ05_000183"
     CDS             83364..84284
                     /locus_tag="JNZ05_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HAY8563547.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGSWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            84456..84653
                     /locus_tag="JNZ05_000184"
     CDS             84456..84653
                     /locus_tag="JNZ05_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HAY8563548.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            84677..>85595
                     /locus_tag="JNZ05_000185"
     CDS             84677..>85595
                     /locus_tag="JNZ05_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY8563549.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYWDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEEILRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGIIKCS"
BASE COUNT        20528 a        22802 c        21088 g        21177 t
ORIGIN      
        1 tctgcctgga atgtaccaaa cgccatcagc gccgccacgc tcgggtctat cttgttcggt
       61 gattttttct tgttcggctt gatattggcg ttcgcgtcac tctgcatcac gacgttactc
      121 atcgaccagg acaacaccgg atcgccacga tgcacaatca ccctgcggtt aacaaaaact
      181 tcgaacgatt tcgccgccgg actgaatctg aggtaggttt gcgggaacgg ctccacctca
      241 aaaccagccc cctgtaattg cgtcctgaga tgcgtggcgt tccacgtatc aaagcccacc
      301 agcctgatat taaatttctc cgcatcctgc atgatgtcat ctctgatccg gtcgtaatca
      361 atgcagtcac ctggcgttgt gcgtatccag cccgctttag cccactggcg atagacagcg
      421 cggtttttat tggcggggtt ctgtagctgg aactccggca gataatgacg ggaaatcagc
      481 ataatggttt taccgaccgg aaaggcatag catacgctgg aaatatcgct ggttgatgat
      541 aagtccagcc ccgcgtaaca ctcctgaccg tgtaaatctt cctccgtgaa cgttccggca
      601 cactcagccc atgcaccatt acccatccac ggggtagtcc cctgacacca gatattaaat
      661 cgctttgtca tcatctccac ccactgcgac ggaatacccc gcgctttctg gatggttgag
      721 gccagttttt cacgatccac ggaaacatca atattagggt tcgcctttat ccacatcgcc
      781 ggatcatcaa cctcgttttc gtcgtccagt tcgtaaatca gtacaaaaat tgaatcgttg
      841 acctcttcgc cgtccaggat ctggcagcaa taatcatagt gctgtttaca ggctgaaacg
      901 acgttactgc ccgatgtggt aatggcaaat aacagccctt cgggacgtgc gcccatcccc
      961 agctcaagcg cggaataaac gccgttatcg gggtgtaggt ggtattcgtc cacgatggaa
     1021 agactggggt ttgtcccctc gatggttgcc gcttttgctg ccagcggctt taacaggctg
     1081 ttgcttttcg ggtgtatcac cttatgcgcc tgaatattca cccgcctgcg taacggtcgg
     1141 gataaaaggc acatctgacg cgcatcatca aacacgatcc gcgcctggtc acggctcacc
     1201 gctgcggtgt agatatcctg ctgaccgttc tccataatca gaaaccagtt agccagaatc
     1261 gcggcggtcg tggatttcgc atttttgcgc ggcacttcga taaaggcgct cgtgtatttg
     1321 cgccgtccgg tggccttaac cttaaagccg aggatgcacg caaaggcgaa ctgctgccac
     1381 ggctccagct caatgggtct gccacgcatc ggtcctttta cgtgcgggca caccctggaa
     1441 aaggcaataa accgctccac aacctcacga tcgaacatgt aaagggggct tttaaggtcc
     1501 gaaaagtacc gtttaacagc ctgttttaac cgtttacagg ccggaatttt gcccgttttt
     1561 acgtcttctg cgtacttatt ccaggcggtc aagctcgtcc tcttcttctg tttccggtgg
     1621 atttttacgg cggcttatcg ggtcaaaacc gagcaaggag gccattttta tcatcactct
     1681 ttcagcgtcg gattttgcgc ttaatgcggg gtttctgctc tcgccgccct gactgttaac
     1741 aatgctgaac ccgcgcgccg caaggtctgc aacggctttc cggtaaatgg attagttgac
     1801 gcaatacagt tccagattgc tccagtcggc gggggtcagg tctccccgtt ccgcaagctg
     1861 ccgcgatttt tcccgccact gcttcacggc gatatcatcc aggtaggcgg gggctttcgg
     1921 tggtcttgcc atgcttattt ttcgtcagat tatttttcaa aaaattcccg tgcataaaaa
     1981 tttggggagg cgttcggtgc ccggcggggt cgggtttgtc ctgaaaacgc cccccacccc
     2041 gtcatacagc ctcatcagcg attgcggaaa cattccataa cctcgcggtc acggtcggtt
     2101 aatcgcttcg ctgtgatgcg ttctgcgcgt cctgacgctt tatctttatg ccctgtttcc
     2161 tgtgtcttcc atgcgtcacg ctgccttata agtccacgga taaggcggtt ttgttcccgc
     2221 tcattcatca gcgtcataca tccagttatt gcggttagca gcccgttctt cctcttcacg
     2281 aaatccacct gcggcacgct tgctttttgt tgccggatca agccatttcg ttttctggtt
     2341 atgacacgcc tggcacaatg gttgatggtt ccattcgggc cagaagagaa catcatcacc
     2401 gccattaatc gggataatgt gatccaccac cacggcgggc gtatatatcc ccttctcaag
     2461 gcatcgcacg cataacgggt ttttactcag atacatggcg cggtatttgt cccactgtct
     2521 ggaatatcca cgcgcgcggc ggtgtcctcg tctggcatcc tctgcacgcc atgccgcccg
     2581 cctgtgctcc tcacacttgc cggatttaac gcgcctgtta cagcctggtt ctgtgcatcg
     2641 tcttaatggt tgccacggca tcagtacacc cccacatcac ggtaagccgt ccagagtgcg
     2701 cctatcgtca tgggtacgcg tgtttttgcg ttatccgcga caatctggcg attttcatac
     2761 aggtgagcga taaacatcat gcagccaatc tttatggctg gcgtgaacgc cagcccgtca
     2821 tcaaagcgcc tgcctatgtg tatctggcac gcctcaagcg atgcggcaat gtatccgctg
     2881 attaactggt cttcatcgtc gccatcgatg cggcgatgga gtttcacttc ttccagggtg
     2941 ataagttctt ctgtcatttt tccgcgccct cacgacaaag aatttcaagg cgtgtcctgg
     3001 cggcatccgg cagcggctgc ccgatgatat tcagtacacg ccccgccagc ggccccgtac
     3061 tgacctttat ccggctggtg gcgttgatgt ccttccggta ccgtatccag atccttacgg
     3121 taccaatcgc cagctctgcg cccgatgaaa tggcctcctt gctgctgacc atgttcacgc
     3181 ttgcccagag tgtgtgaccg tcctcccacg tttcgagtat ttcgccggtt atatccctgg
     3241 tctgtttcag ggtctgaatc gttatcctgt cacgcagtcg ccctatgttc attccggttt
     3301 ttctccctcg ctgattttta cttcctgttt ccatgcctgg ctgaactcat caccaccatc
     3361 acgcggtgac agcccttcac gttcgcgggc ttcgttcgga cacataacgc cggattttat
     3421 gccgcgctca taggtggcaa agcgttcgcc aggtgtggcc cgtaacaggt ccgcgctgtc
     3481 aaactccacc tgataccgga taccaggtac aggcgaggcc accagcaagg cattttttat
     3541 ctgctgctca aagttcgcca gccacgggcg catcgtcatg gtgagaaatg cgcggcttgc
     3601 ctcgctgaag ttgctgtagg tgctgttgct gtattcctgc aaaaaaatcg gcgagacgtt
     3661 gaacattcgg gcgatgtctt caatggtgaa gcgacgggag gccagccatt cggcatcctg
     3721 attactcatg cccagctgct gatagctcat ccccccttca aggatgggcg tttttccggc
     3781 gtttctggcc cctttgtagc gttccagtgc ggctaatgcc tgtttgccct tcacgccgtc
     3841 cagccattcg cctgacgtga taacccctgc cgccatcatg ccatcacgca tcacgctcgc
     3901 gccgtggcgt tgttgggcca gcccaagccc cagcgattcg cggcagatgg ttacaggtga
     3961 gcgccccata aaaccgtcat ccgtggagta acgaaggtgg agaacctccc acggtaaata
     4021 gttgcgggtg tttccggtgt aggcgtcagt gatgcagtag cgccagttat gatcacctgt
     4081 ctgctccacg ttcaccgact gcggcgggta aggatgtaaa gccgccggaa aaccatcacg
     4141 cccccactga ataaccgcat aagcattacc acttaacagg cagtggcgga tcatcattcg
     4201 cttaaactgg tagggtgtct gccacgcgtt cgggcgctcg ttgaggatgt gatcgaccgg
     4261 atgagaatca agccattcgc gggcctcctt ccccttctca ttgcgtacca ggtacaggta
     4321 acacggcatg gtagccaccg cctcagagat gaccgtgacg gcgttcatga cggcggagag
     4381 tgattccgct gtcccagatg atacgtactc acccgccccc gtgttcgatg tgccggccag
     4441 cgccataaat tcatcaagcg tcatgctgcg ctgctctttt tttcgtctga aaggccacat
     4501 atcacacccc cgctaaatcc gcccaccagc ggcgattatc ctcacgcggc attttttcgg
     4561 ggtgctgctc atacagggaa cggcgggcca gctccacgcc ggaatcgggg taagccggta
     4621 ccgatgtaac ggtaatttcg tacagttccg ccaccagcac ggtgcgcacg catggatctg
     4681 ttgtggtatc ccatacatcc ttacgggagc gaaagctaaa gctcatcccc gagatgtccc
     4741 cacgcttaac cagttcgata acgtcgcgcc ctgtactggt atccggtggg gtcagttcaa
     4801 agcgtaaccc tgtttcgtcc tcttccagtt tcagcgtgcc ggaacgggtg cgccccagta
     4861 acatgctgtg gtcatgctca tacaggccgc gaacgtcatt acccgccgca agccattcag
     4921 taaacgcccc ccgctggaat ttttcgtaaa actcacccca taacagttct gaaagcttat
     4981 cccagcgaac aacgtagccc gtcagcgtac cggctccgct ggtggtgatt tccgatgacc
     5041 ggatttccat actcttcata attttttcac ccataaaaca ctaaaggggc tttttagccc
     5101 cttctgtatg ctgttaatcg tcgtcttccg gcagttccag aatcttgatc gcgttcgaat
     5161 ccaccacgcc accgcctaaa tatttttgcg tgaaaatttt gatgaatcct ggttctgtca
     5221 ggttgtccgg tctggtgcga acacctgttt cgtgatcaac aatgtagtaa ccgcgtttga
     5281 agtcacccag ggcaataacg ttatcaggca taaactccag atattcgacc ggaaggccca
     5341 gcaacgtatc aggatcaccc gcctgtaaac ggtcgcgcca gatataatca ccgttcgcgt
     5401 tcttcacctt ctgaagtttt gccgccgtcg tggagttaac cacccagacc gcgtttttgc
     5461 ggtatttttt acgtaatgca aatttcaggt cgatcagcgg gtccgcagat gtccacgcca
     5521 gagattcgga aggtttaatt acctgcaacg taccaaaatc acgctctttg tcgttcttct
     5581 ctgcacgggg tacggataaa aaaccttttg cttttttgtc accgtcgccc acaaccagat
     5641 cgctttcttc ggtttccgtg aaggtgttgc caatctcacc cgtcagccat gaaaggatgt
     5701 ccacatcgga aaaatccacg atttcctgtg tggtgcgcgg gtacgcatag accggataca
     5761 gcttaatgct cacctcgtta atctgcgggg tgctggtctg ttcgcgtgcc ttaccctctt
     5821 caccgtggtt aacggtcgca ccgccagcgg aaacaagctg cttaaactcg ttgctgctga
     5881 ttttcttcac ggtacagatg cggcgcatgg tggattcatc cgccagcatt cgcatgattt
     5941 cggtgttcag ttcagggata acggtataac caccatcagc gggaacgcct gtacttaatg
     6001 cgcgggtttc accggtcaga atgtagttgc gtagttctgc ggggtcagtg gtctggctgc
     6061 ttttacctgg cttgctgcgc tcttcgtctg caatggcttc aaggcgggaa atgtctttat
     6121 cgagggattc agctttagcg cgtaattcgt caaattttgc gccctcagca tcgttaagac
     6181 tgcggttttc tttttcagcg ttctccagca tgtcgcgcat ctgattttta atggcggttt
     6241 tctgctggcg taattcgatt attctctgca taaaaaagtc ctggggttaa gtaaggaact
     6301 ccaggacgcg gcaaacactc aaccgttttt cataaggaaa tcagtaatcg caccgatcgt
     6361 tttcccgcct ggtaatgaat attggcgagc acattaacag gcgggaaagt ggccccagcg
     6421 tcctggcacc acgggcgaga ataatcatga ttcagttcgg gtaaaatatg ccgatccgct
     6481 cagtgaacaa cgtggaacaa ccacgaacaa ataatttaca aaaaataaca aaaagccgga
     6541 ttgctccggc tgcgtggtgt ggttctgata ttcctactct gctaatttgc tcagccccat
     6601 gtctgcgtaa gtgcgattta ctgcatttct caggtctgcg tagttctccg ttggcggctc
     6661 cggtgtgccc ttgctgctct gttttaccag cgggtcgccc accagccgcc cgtaagctgc
     6721 aatttgtgct gtcatattgc cacctcagtg aagcgatccg gcattgttct ttttcatctc
     6781 ttccatcatt ttttcagtga gcattacgca ctgaaacccc atgtttttga tgtctacggg
     6841 tttcatgcct ttgtcagtgg atatcacagg cagaatatcc tgatacacat cgcggtcagg
     6901 gtaactaagc acacaaagcg cccatgatgc cacgggtgag gatccttcgt ctgtttcaac
     6961 ccaccagcct ggtgtagctg cgcttatttg cagaatcgtg atccgttcgt tgtgcttgta
     7021 aaatttttca ccaaattttg gttcgctcat gattccacct ctccggtttt aacgttgatt
     7081 gttgttacct gttccgcttc ggcaatctcc cgctctgtca gcgtggcaaa atttgccgcc
     7141 gtcgtggtca tgaatgcgct tatcagttcg ggatgtgctt tcgcgtatcc ttcccccgcg
     7201 tggcggtcta tcgttctgat tgccaccttt aaagcgtgct ctgtcatgtc taacgcgcga
     7261 tatttcggtt ctgttctgtc tctgcgtttt ttgagtgatt tattaaattt ccctgaagtg
     7321 tgcatattta tttttacccc ctcgtttaaa aagttttgag ttgtgcctcc ccttgtctac
     7381 cttagtggcc ctcatgccag taatggcgct gctttcagcg gggtagagtg cttttatcca
     7441 ctatctaccc cgtgtctacc tccctgtctg attcaggtaa aatcaggtag acagggtaga
     7501 cagcaattac aaaaaattat aaaaacgcgt cgcactcgtc tgttgttatt gcgttagtct
     7561 gcgttactcc cttaactttc cgcgtaatat attcatgtcc gtaaactttc gcggctggct
     7621 tcatagcctt gccaaagtca tttacgttta gcggtttgct cctgcctgcg tacgccataa
     7681 acgccagata gacgcggtaa aggctgtttc tggtcgtgta cttcactgaa tcgccaccgc
     7741 cgcccatcat caggccgcgt gcttcctcca gaaaattcag gaactggcaa aactcaataa
     7801 ccggatccgt ctgttgcttt attgccagag cttcatcacc gtcacgctgt tccagtagta
     7861 aagcccgtgc tttctcagag tcggtaaagt tcgccagcaa tcggcggata atgacaggga
     7921 tttcagccgc aatcttttcc ggtagctccc tgtctttttc ggcctcacta acgatattgt
     7981 cgaaacggaa aatcacgcga cgacgtgcca cacctccggc ccgttcggtg aatatcatcg
     8041 ggttattgtt ggtcgccagc accaccgccc tgattacagc cgtgaaacgc ttttcgtatt
     8101 tcgggttaat ttccacgggg tcaccgcccg tgattttctt gatgcccgtt ccttcgcctg
     8161 tatatttcgg ctggtcagcc aggacgataa gacgactccc gacaacctgc ggacgtccac
     8221 cagcatcatc aagcgatgtc atttcagcgc ttaccgtgtt ctgtttccct gccagaaggc
     8281 tggctatgtg tgtgaatgta cttttaccgc tcccgccgtc tccggtggcc tcaataaaca
     8341 tctgccagtc gtaccggttc gccataatca tgtacagcgc ggcacatata cgcatcatct
     8401 tgcgctggtc ttttccggct gcgtgctcaa gccatttatg aaagtttagc gcgttatcgc
     8461 ggatgttctc ccctggtgct ggtggcgtgt actcaatgcc gttgtgcgtg gtgatccagt
     8521 tctccggcgt gtgcggggaa aattcccccg ttttcaggtc aagcgcacca ttggcgaacg
     8581 gcagcaaatc gccggacggc tcgcccattg gttcggcaat aacttttaac gcttccacgg
     8641 cgttattgat tacgcgcttg ctgaaagtgg ccctgtgctc tgaatagatc gccaccattt
     8701 cgcggctaag ttccattgtg ctgaccggac accatacccc gccgcgccat acgtgaacga
     8761 tttcactttc ctgatgcaca caaacgccat caaagcgctc ggcaagcagc tgcgcgcgct
     8821 cactgtctgc catttgtgcc agttgcgtat ttttctgctc cggcatggta agccctgcgg
     8881 caatattttc acgctcagca ttcagatagc gccgccagtt ttcacacttc tggacgtgca
     8941 ttccttcggg gtaaaaattt gcgtcctgaa tatctgccgc cgccagcttc tgaccaatct
     9001 ttttggtctc caccaaatcc agttctccgg cctggtacag ccttacgcgt ttttttccat
     9061 ccggaacaat tttcagtgca tcaagttcgg caagctgatt tggcccaagc cacacaggcg
     9121 gcacattatc gccggatgcg gggccatcct gctcctgcca ttgtttcgcg tgcgaccagg
     9181 catcactacc cgcaaagata attacctctg tgtctttgtg ttttattccg cgtggctgtt
     9241 tttttacgtt cggtgccagt ttcatttttt acccctgaat acgttaagca tcttttttat
     9301 ttcctgaata ttggcgcgtg ctttctccct gctggttggt ttactgcggg gcgctgcctg
     9361 taccagcgtg aaatcacgcc ggaattgata aacaggcatc acgcaatcat attcgtaacc
     9421 ttcacggcgg taggtgatgc gccgttctgc cacgccttta atcgttacca tgccgccgta
     9481 ctggtcgcgg taaatatcac cgcgcgtaaa tttagggtga gtgttgccac tggcagttaa
     9541 gccagaatat ttaagtttca ttatttttat tctccggtgt gctgttcttt atatctgtcg
     9601 tgcaatagat ctatttcttg cagttccatt attacaggct caagaagcgt tattaatgcc
     9661 gtggcaattc ttgatttttg tttgtcgcgt tcattgtcgc caagtgtttc aagccatatg
     9721 cgcaatattt ccagcatgtt ttcactgtga gaaagtgcaa gaaatgcgcg gtctattgtt
     9781 tcgtggtaaa tatcacgcat attaatcccc gtccgtcgtt tttcttaaaa taacctctgt
     9841 cacgaaatca gcataatcgg cggcgatatt cagtacatca agcccgttga tttatattct
     9901 ctcgtggaaa gtaagaaaaa agccgctcta ataagttctg gcattgacga aagcgcatca
     9961 gccgcatcat caggaacgcc ggaaaattcc tgtttcaggg aattaaaacg atcatcacgc
    10021 ataacccccc ccattttcac aatcggcaac aagaatattt ttagcgtcat tcagtgaccg
    10081 cgttgcgatg tatcggatac attccagggc gaactgtgtc cggtctttct gttcttctcc
    10141 ctgcgcaagc tctgccgtgc attcaatatc aataagcgcg tgcatcagcg tagtgagtgc
    10201 ggcggctgct gcgttcggtg tggttttatt gtgcataggt ttccccctga cgaatacggg
    10261 cggcgaatac agcaacacaa ccggacgggc aacggctacg cgcttcgcgt tccgtccaag
    10321 cggttacgtg gatgatttgg gattctccgg tactcagtgc cagaaaacgc cacacaaagg
    10381 ccgtttgtgt gtgtacaagg tgtggtatat gatttacggc aaccataacg gctcctagtt
    10441 tacgttgttg gttagaagcc ccgttactgc tcctacagtg cggggctttg ctttttgtgt
    10501 tgattgtgtg taatgtgtca ttacacataa atacattaca tgaggtgtaa ttaacgtgtc
    10561 aacacacaaa aacgaaaggc gtggtaatcc tccgttccag ttccgtttag atcctgactt
    10621 acgggaaaag atggaaaagg cacaacaaca ggacggcgat gattctcttg ctgcctggat
    10681 aaagcgaata atccgcaaag aattacagca acgcggcata gaacccaaag gataaaaact
    10741 atcagcgccg tggtgtgagg tactacggcg cattgcttta caggcgcaca caatgaccaa
    10801 cgaagaatca accgaaaaaa catcacctgc acgaaaaaga cgacgcagaa agaaagagca
    10861 tgaatcagaa cgattcgcgc cgtgttcttt tgcccttgag aagtacctta aagaatactc
    10921 atcaaaggag agaacttcgc agttatggca acgcactgaa ccagactgat tgcattgccc
    10981 accagcctga tagcggctat catccccgtg cttatgtttg ggatcacaca cacaaacggc
    11041 gcagcgggtt atctgttcag aaaggcggct ccatttcggg gccgcttttt ttatgcctga
    11101 aaaaccccaa ttttgtggtt ttccagtttc accagggcga acgaatcccc gcccacgttc
    11161 tggcgtatat tcaatcttca tggttatagc tctgtgttca gatgattgat gtgtggcggc
    11221 tgcgtgccgc cagcgtgatt aatgaactgc cttgcagcta tccttccagg ccagaacctc
    11281 ggataaagac cagccaacgg aacgaccgcc aagtttacga cgtgatggga attgtccggc
    11341 cttttccagg cggtagcggc atgagcggct aaggcctgtt agcttttcgc attctttttc
    11401 acgtataaac cgatcagtgc ttaacactat tgcccccttt cgtttcttaa agagttattt
    11461 cgtgttctat tgcgttggga tgtgtctgat tgtgtcagga tgattcagag ttggcaaatg
    11521 ttgaggccgt atggtttaca gaaagaggaa caatcaggat aaaatcattt aaattcatgt
    11581 taatacaaag gcataaaata ttgtttcatg ccttttttct cgctatttaa agagtgattc
    11641 gctagtgtat aaaaaaccag taacacgtta aaaatcagct acttataaat ccgtacactt
    11701 tttcacctct tgttcgtagt tgttccgcat tgttgctcat tgttgcacgt tgtatctgtt
    11761 cgcatatcca gtatgcgcat actgaaaaaa cacgaaaaaa attattttct tctggctact
    11821 ggtagcgtgg ttacgttttc atgtgttccc gccagtatcc ctaaccgctc cgtccacata
    11881 tccagcgcat tgcgtttagc atccagataa cgggaatgat tataaactcg ctgcattcct
    11941 ggcatctggt ggcctgtaag ctgctccacg acgtgaggat ccacgcctaa atcgttcagc
    12001 atcgttgtaa aggtgcgccg gatgtcatgt agtgaccagt gagggtgatt tagcctcctg
    12061 tgcgctaatc tgccgtactg cgacacgctt gtttcctgtt tcacttcccc cagcaataag
    12121 cccgtgtgcc tgttctgctc caccagctgc gtgacgaacg gcagtattgc ttccggtatg
    12181 ggccggaata ttgccacctt cgttttgctg tgttctttcg gcacggtcca gagcatttcg
    12241 gtaaaatccc actcgctgat ctccgataac ctcagttcta ccgtccggca tccgaacaca
    12301 atcaggaggc ggattaacgc gatgtagtag ggggagaata tttttttgtc cagtgcctgc
    12361 aataattcgc ccagttcttt ggtgcttaag acacgctcgc ttatatccgg ttttttccca
    12421 acgtccgcca cactcatatc atcaagaacg ttgctgattg catagcgccg cctccggcag
    12481 aacttaagcg cctgtttgca cgtctgtagc aagaatccgg cagtaacagg cgttcgcttt
    12541 gccacctggt caaaacaggc cagccagtgc cgtagctcgc atttatccag cggcatagca
    12601 ccaatgtgct gtattacgtg attattaagg cgctttttca gggcggcata atccacgcgg
    12661 ttttcctttg cgtacgactc aagccagtag gtgagcgcat cgccaaccgt taccggcttt
    12721 aacgcttcct gtacggtgta attaagctca tgacgtggat ttttcccctc tgccagccat
    12781 gcgcgacact gggcggcttt ttccctggct gatttcaggc tcagatcagg ataatttccc
    12841 agcttaatgc gttccggtgg tgcccccctt cccgttccgg ccctgtaagt gaaataccag
    12901 gttaaaaggc cactggttga atgcctgacg ctcaggtttc caccgtcatt aagaaaggct
    12961 gttttttggg cgggtgtgcc gttgattttc ctcagctgtg tatcgctcag tttgttaagt
    13021 gctctgctca taattttgat tccggtcatc acaattaact acaccattaa ctacaccgat
    13081 cgttgcacaa agggctacaa cgtgaaacaa gctggaacaa ggaaaatcac aaatctgttt
    13141 ataatcaaaa acataatgaa caatctgaaa cattatgaaa cggcaaaaaa cactaattga
    13201 taaaatatgt ccatacttca cgcattacgt taagcatccg ttataatcgg ttgcagatac
    13261 cagcctgtgg atgcttaaca tggaatacca actcactctt aactggcccg attttcttga
    13321 acgtcactgg cagaaacgcc cggtggtgtt aaaacgcggc tttaataatt ttattgaccc
    13381 gatctctcca gacgagttgg caggtctggc gatggaaagc gaagttgaca gtcgactggt
    13441 cagtcaccag gatggcaaat ggcaggtcag ccacggcccg ttcgaaagct acgatcatct
    13501 cggtgaaacc aactggtcat tactggtaca ggcagtaaac cactggcatg agccgaccgc
    13561 cgcgctgatg cgaccgttcc gtgaactacc ggactggcgt attgatgatc tgatgatttc
    13621 tttttctgta cctggcggcg gcgtcggccc gcatctcgat cagtacgacg tgtttatcat
    13681 tcagggtacc ggacgtcgtc gctggcgagt gggcgaaaag ctgcaaatga aacagcactg
    13741 cccacatcca gatctgttac aggtcgatcc gttcgaagcc atcatcgatg aagagctgga
    13801 gcctggtgat attctttata ttccgccagg attcccgcat gaaggctacg cgctggaaaa
    13861 tgcgatgaac tattccgtgg gttttcgcgc gccaaatacg cgggaattaa ttagcggatt
    13921 tgccgattat gtgctgcaac gtgaactggg cggcaactac tacagcgatc cggatgttcc
    13981 acctcgcgct catcctgcgg atgttctgcc gcaagagatg gataaactgc gtgagatgat
    14041 gctcgaattg atcaaccagc cggaacactt taagcaatgg tttggcgagt ttatatccca
    14101 gtcacgtcat gaactggata tcgcgccgcc ggaaccgcct tatcagccag atgaaatcta
    14161 cgatgcactg aaacaaggtg atgtgctggt gcgcctgggt ggtctgcgcg tattgcgcat
    14221 tggcgacgac gtgtatgcca atggtgagaa gatcgattcc ccgcaccgtc cggcactgga
    14281 tgcactcgcc agcaacattg cgctgactgc ggagaatttt ggcgatgcgc tggaagatcc
    14341 gtcattcctc gcgatgctcg cggcgctggt caatagcggg tattggttct tcgaagggta
    14401 agtttggatt taagccggat gcagcatcac atgccgcatc ccttttcgct tacttccgtt
    14461 gcgccgttaa ctcggcaata cggacgatca cctgcaccgc tttttccata ccttccagaa
    14521 tcacaaactc atgcttacca tgatagttgt aaccgccagt gaacaggttc gggcaaggta
    14581 atcccataaa cgacaactgc gcgccgttgg taccgccgcg gatcggtttc agttccggtt
    14641 caatatcgca gtcacgcatc gcctgctggg cgatatcgag aatatgcgga tgctcagcca
    14701 ctttctcgcg catattgtag taactgtctt caatcaccaa ttcaatgtag caatcaggat
    14761 gtaacccttt gcccactttt ttggcgatct ccatcatttt acgtttacgc gcttcaaact
    14821 gtttacggtc gaaatcacgg atgatgtagt gcatatcggc ccgttcaacg gtgcctttca
    14881 tgctcgccag gtgatagaaa ccttcatagc cttctgtcat ttccgggctt tcatccgccg
    14941 gaacttccgc atgaatacgt gccgccagcg acagcgcatt taccatcact cctttcgccg
    15001 tgcccggatg aacattgtta ccgacaattt tgatattgac cgatgcggcg ttgaagtttt
    15061 caaactccag ttcgcctacg ccaccaccgt caacagtgta agcccagcgg gcatcgaagg
    15121 catcaacatc aaaatgtttc gcccctttgc ccacttcttc atccggggta aaggcgacgc
    15181 gaatatcacc atgcggaatg tttttctgtt gcaataccgc cagcgcggtc atgatttctg
    15241 caatacctgc tttgtcatcg gcacctaaca aggttttacc atcggtggta atcaacgtct
    15301 gacccagtag ctgatgcagc accgggaaca taaccggtga taaaacttca tcgccgatac
    15361 ccagcgcaat atcgccaccg cgatagtttt caacaatttg cggattaaca tttttgccgc
    15421 tgcaatccgg tgaggtatcc acatgagaaa taaagccaat cgccgggata tcgccaggga
    15481 cgttagccgg taacgtcgcc atcaaagtgc ccttctcact taaggtcaca ttgataagcc
    15541 ccatttcttc gagctgctct ttcagcagat gcaataactt ccattggcct tccgtgctgg
    15601 gaacctgtct cacccctgct tttgattggg tatccagaga cacgtagttc aaaaatcgct
    15661 caagtagttt atccatgtag tcaccctcac tttttgtgac aacattatta agaagcaaga
    15721 aaagacaaat attgcgtcag gtcactttta cccctgcaag cgggaatatt tatcagcatt
    15781 accttaatga atataaagct aagccagtaa ttcacaacaa agattggcga ttcaagcggt
    15841 ttgccgtaga attaccgccc tcattaagag tcaccaaggt ggttaaccac aaaccccgca
    15901 tcggtaagcc atccgttgcg tttacatggg acagagtaaa aaattgaata aacaaccgag
    15961 ttcgctttca ccgctggtgc aattggcggg aattcgcaaa tgctttgatg gtaaagaggt
    16021 cattccccag ctggatctga ctatcaacaa tggcgagttc ctcacgctgc ttggcccttc
    16081 tggctgcggt aaaacaaccg ttcttcgcct gattgcaggt ctggaaactg ttgattccgg
    16141 acgcatcatg ctggataacg aggacatcac ccacgttccg gcggaaaacc gctatgtgaa
    16201 cactgttttc caaagctatg cacttttccc ccacatgacc gtgttcgaaa atgtggcctt
    16261 tgggttgcgc atgcaaaaaa cccccgctgc tgaaattacg ccccgcgtga tggaagccct
    16321 gcggatggtg cagctggaaa ccttcgctca acgtaaaccg catcagctct ctggtggtca
    16381 acagcaacgc gtcgccattg ctcgcgcggt ggttaacaag cctcgtctgt tgttgctgga
    16441 tgagtcgctc tcagcgctgg attacaaact gcgtaagcaa atgcagaacg agctgaaagc
    16501 gttacagcgt aagcttggca ttacattcgt tttcgtgact cacgaccagg aagaagcact
    16561 caccatgtcg gacaggattg tggtgatgcg cgatggtcgc attgagcaag acggcacgcc
    16621 gcgtgaaatc tacgaagagc cgaaaaacct gtttgttgcc gggttcattg gcgaaattaa
    16681 tatgtttaac gccacggtga tcgagcgact cgacgagcag cgcgtacgcg ccaacgttga
    16741 aggccgcgaa tgtaatatct acgttaactt cgccgttgaa ccggggcaaa aactgcatgt
    16801 tctgctgcgc ccggaagact tacgtgttga agagattaac gacgacaacc acgctgaagg
    16861 gctgattggt tacgttcgtg agcgtaacta caaaggcatg acgctggagt cggttgttga
    16921 actggaaaat ggcaagatgg tgatggtcag cgaattcttc aatgaagacg atcctgactt
    16981 tgaccactct ctcgaccaaa aaatggccat taattgggta gaaagctggg aggtcgtact
    17041 ggctgatgaa gaacacaagt aagttccaga atgtagtgat tgtcactatt gtcggttggc
    17101 ttgtgttgtt tgtctttctg cccaacctga tgatcattgg caccagcttt ttgacccgcg
    17161 acgacgccag tttcgtcaaa atggtcttta cgctggataa ctacacgcgt ctgctcgatc
    17221 cgctctattt tgaagtgcta ttgcactcgc tgaatatggc gctgatcgcc accctcgcct
    17281 gcctggtgct gggctatcca tttgcctggt ttctggcgaa gctgccacac aaggtgcgtc
    17341 cgctgctgct gtttctgctg attgttccgt tctggaccaa ctcattgatt cgtatctacg
    17401 ggctgaaaat tttcctcagc accaaaggct atctcaacga gtttttgctc tggctgggcg
    17461 ttatcgacac accaatccgt atcatgttca cgcccagtgc ggtgattatc ggtctggttt
    17521 acattctgct gccgtttatg gtgatgccgc tgtactccag tatcgaaaaa ctggataagc
    17581 cgttgcttga ggcggcgcgc gatctcggtg ccagcaagtt acagactttt atccgcatca
    17641 ttattccact gacgatgccg gggattattg ccggatgtct gctggtgatg ctgccagcga
    17701 tgggcctgtt ctatgtgtcc gacctgatgg gcggtgcgaa aaacctgctg atcggtaacg
    17761 tcatcaaggt acagttcctc aatattcgtg actggccgtt tggtgcagcc accagcatta
    17821 cgctgactat cgtaatgggc ctgatgttgc tggtttactg gcgcgcttct cgtttgctga
    17881 ataagaaggt ggaactcgaa tgatcggtcg actgcttcgc ggcggtttta tgaccgctat
    17941 ctacgcgtac ctgtatatcc caatcattat tttgattgtg aactccttta acagctcgcg
    18001 ctttggcatc aactggcagg gttttaccac caaatggtat agcctgctga tgaacaacga
    18061 cagcctgtta caggcagcgc agcattcact aacaatggcg gtgttttcgg cgacgtttgc
    18121 tacgcttatc ggttcactga cggcagttgc gctgtaccgt tatcgctttc gcggtaagcc
    18181 gttcgttagc ggaatgctgt ttgtggtaat gatgtcacca gatatcgtga tggcaatttc
    18241 gctgctggtg ctgtttatgc tgctgggtat tcagcttggc ttctggtcgc tgctgttctc
    18301 gcatatcacc ttctgcctgc catttgtggt ggtgacggtg tattcgcgcc tgaaaggttt
    18361 tgacgtgcgg atgctggaag cggcgaaaga tctcggtgcc agcgaattta ccattctgcg
    18421 aaaaatcatt ctgccactgg caatgccagc ggtggcggcg ggctgggtgt taagctttac
    18481 cctgtcgatg gacgatgtgg tagtttcttc attcgtcacc ggaccaagtt atgaaattct
    18541 gccgttaaaa atttattcga tggtcaaagt cggcgtatcg ccggaagtta acgcgctggc
    18601 aaccatatta ctggtgctgt cgctggtgat ggttattgcc agccagctta ttgctcgtga
    18661 taaaacgaaa ggtaacacag gggacgttaa atgaaaaaat ggtcacgcca cctgctcgcg
    18721 gcgggtgctc tggcactggg catgagcgcc gctcacgccg atgacaacaa cacgctgtat
    18781 ttctacaact ggaccgagta cgtgccgcca ggactgcttg aacagttcac caaagaaacc
    18841 ggtattaagg ttatctattc gacttacgag tcgaacgaaa ccatgtacgc caagctgaaa
    18901 acgtacaaag acggtgccta tgacctggtg gttccttcaa cctattacgt cgataaaatg
    18961 cgtaaagagg ggatgatcca gaagatcgac aagtcgaagt taacgaactt cagcaatctc
    19021 gatccagaca tgctcaacaa gccttttgac ccgaataacg actactccat tccgtatatc
    19081 tggggtgcga cggcgattgg tgttaacggt gatgcagtgg atccaaaatc tgtcaccagc
    19141 tgggccgatc tgtggaagcc agagtacaaa ggcagccagc tgttgaccga cgatgcccgt
    19201 gaagtgttcc agatggcgct gcgtaagctg ggctactccg gtaacaccac cgatccgaaa
    19261 gagattgaag ctgcatataa cgagctgaaa aaactgatgc caaacgtcgc agcgtttaac
    19321 tccgataacc cggctaaccc gtacatggaa ggcgaagtta acctcggcat gatctggaac
    19381 ggttctgctt tcgttgcacg ccaggcgggt acgcctattg acgtggtgtg gccgaaagaa
    19441 ggcggcatat tctggatgga cagcctggcg atcccggcaa atgccaaaaa caaagaaggc
    19501 gcgctgaaat tgatcaactt cctgctgcgc ccggatgtgg caaaacaggt tgctgaaact
    19561 atcggttatc caacgccaaa ccttgcggcg cgtaagctgt taagtccaga agtggcaaac
    19621 gataagacgc tctacccgga tgctgaaacc attaaaaatg gcgaatggca gaatgacgtt
    19681 ggcgcagcca gcagcattta tgaagagtat tatcagaagc tgaaagcagg acgttaattc
    19741 agcaagcggg cggtggtatt accgcccgtt ttttagttag caaggatgtg gctaatgagt
    19801 caggacagta aagttttatc ccgctctttc gtcggtattg tggtccgctt gtaggcctga
    19861 taagcgtagc gcatcaggca atgcttcctg ctttataatc cctccagcaa cttttcaaca
    19921 aattctggca ccacctggct tgccgggccg taatatttct cggcaaattc attaccaacc
    19981 tgactcggtt caagattcag ttcaacggtg tgagcaccat gtagtttggc ttcgtgtaca
    20041 aacccagcag ccggatagac atgcccggaa gtaccaatgg caataaagac atccgccatc
    20101 gacaacgcca tataaatttc atccatgccg agtggcattt cgccaaacca aacgacgtgc
    20161 ggacgcaagg gtgccgggaa ctggcaacaa tggcatttat cttctggcgt aacgtctccg
    20221 gtccagtcga gaacctgacc actttgcgaa caacgaactt tcagcaattc cccatgcata
    20281 tgaatgacat tggtattacc tgcgcgttca tgcaggttgt ctatattctg cgttaccagc
    20341 aaaaagcgat cgccgagagc gtcttgcagt ttagccagcg caagatgcgc ggcgttaggc
    20401 tgaatttctg gctgctgcag ctgtcgacga cgggcgttat aaaacgcttg caccagttca
    20461 ggatcgcgat cgaaaccttc cggcgttgcc acatcttcaa cccgatgttc ttcccacagg
    20521 ccatctgcgg cgcgaaaggt acgaatacct gattccgcag aaattcctgc ccctgtcagt
    20581 acgagtactc ttggtttttc cattgcttcc ggcaccactt tatctctgaa aaaaatacgc
    20641 tgacgcaaac gctcgcgcag gcgacgttta tttttacgaa aacgacttaa ccgatgaccc
    20701 cgacgcgaca gcatagcaac ctctgttgtt taatcggtta gatgtaggaa ggccgcacca
    20761 cgcattcctc ccgcatcacc gtggcgcgcg cgttcaatgc gcggaacacg agctacaggt
    20821 aagagatgac aaggcaacct gtccgccagt tgcgttgtga ttgccgggaa attcgataag
    20881 ccaccaccaa tgacgaccag gtcagggtca acaatggtca ggatatttcc cagagaaacc
    20941 gctaataaat ccagataacg ctcaacgtgc gcccttgcct gctcatcgcc ttgatcataa
    21001 agcgcaataa tttcgggagc ctgcaacggt tgatgataat agtgttgata cagccacgca
    21061 aaaccgcgac cagacagata attttcaatg cagccatgct gaccacagcc gcagcggcgt
    21121 aacgggaaat ccaaccccat catggttaac gcatcaaccg gcagacgcat atggccaaac
    21181 tcgccggtaa tgtagctttt accagtaatc ggtttgccgt tgaaaaccag cccgccgcca
    21241 acgccggtgc cgagaattaa ccccatcacc agtggatatt gcgtaaattc atcctcccag
    21301 gcttctgaaa gggcaaaaca gttggcatcg ttatcaaggc gtacatcgcg atcaagacgt
    21361 gcgctcaggt cggcacgcag cggtttgccg ctggcagcgg ggacattagc ggcatacagc
    21421 ttaccatctt ctgtttccgg cattcccgga ataccgatgc cgacagaacc tttacagcca
    21481 aaacgttggt cagcttcagc taccagctca cacactgcat ctaaaaatgc gtcatagctg
    21541 tcacgcggtg tcggcacccg cttttcccac tgcaactgcc gaccgctatc aaacacgcca
    21601 agcgcaattt ttgttccacc aatatcaaac ccgtaataca ttgccgctcc ttgttttaat
    21661 gtactgcctt tactggccgc taaggactcg cgcagggtca atattactgg cgcgccgcgc
    21721 cggataccaa cttgccaaaa gactcagcaa caatgctgtg accagtacgt agaagacgtc
    21781 cagccagtgc aattccgatg gcaggaagtc aataaaatag atatcgctgg agaggaactg
    21841 atgaccgatc agcttttcaa tccactcaat aatcggggta agttgcagtg aaacaactac
    21901 gccgataacc accccacaca ggctgccgaa cagccctgcc agcaatccat accagacaaa
    21961 gatggcgcga attaaaccat ctttcgcccc cagcgttctt aatactgcga tatcgccact
    22021 cttgtctttc accgccatca ctaaggtgga gacgatgttg aaacaggcca cgccaatcac
    22081 cagtaccatc gccagataca taatggcgcg gatcatctgg atatcgcgat acatatagcc
    22141 gtaagtacca atccaacttt taatataaac atagctgttg gtcacttcac ccgcatcgcg
    22201 taccagctta ttggcgttga aaacatccgt cattttaagg gcaatacctg acacgctgga
    22261 acccatatca agatattgtt gggcatccgc cagcgggatc atggcaaaac tgtgatcgag
    22321 ttgaccactc aactgcaaaa taccggcaat gtgcaaacgc acacgttttg gctgcatcag
    22381 tttatgctcg ggattcgagt tggggatcat gatcgacacc caatcgccct gcttcacttt
    22441 cagcgcatcc gccacgcctt tgccgatgat aatttgctgt tcgcccgctt taaaattgcg
    22501 ccacgcatcc ccctgaacaa acgagggtaa tgcgctcaga cgctgttcct gttgtgggtt
    22561 aacgcccttc acctggattg cgcgcagatt cgctccactt tccaccagac cggtgaaatt
    22621 gatatacggc gcagcggcgg caatacccgg cactttctgc acgttatcca gtgcttcctg
    22681 ccagttagtc catggctgat ttaccgcttc gatttcgcca tgcggcacca ccgccagaat
    22741 gcggttattc agttcgcgtt caaagccgtt catcgcgctt aagccgacga tcaataccgc
    22801 cacgccgagg gcaatgccaa tggtagaaat gacagagatt agcgacacca tgccgccgcg
    22861 tcgccgtccg cgactaaaac gcaggccaat caataacgat aaaggcatcg ccattactcc
    22921 gcccccatca ggctcagttc cgccgtcaga cgtccatcac gcatttccag ttggcggctc
    22981 atacgtttcg ccagttgcag gtcgtgagtg accaccagga aagcggtgcc ctgcaagcga
    23041 ttcaactctc caagcaactg gaagatgctg tcggcgttac gcgcatcgag gttaccggta
    23101 ggttcatccg ccagtaccag acgcggatta ttgaccagcg cacgggcaat cgctacacgc
    23161 tggcgttcgc cgccagaaag ttcagatggg cggtgattcg cacgatgctc cagccccacc
    23221 gcttttaaca tctccagtgc acggctgttg atttcagcgg gctttttctt gccaatcagc
    23281 agtggcatag ccacgttttc cagggcagta aaatccggca gcaggtggtg aaactgataa
    23341 ataaagccca gcttctggtt gcgcagttct gctttcgccg ctgaagacag tttgctcatc
    23401 ggctgaccgt taaagatcac atcgccggag gttggcgtat ccagcccgcc taacaggtgc
    23461 agcaaggtac ttttaccgga accagagcta ccgacaattg ccatcatttc accttcgccc
    23521 acgctgaaac tgacattgtg cagcacatcg gtttgcacac tgccttcctg atagcgtttg
    23581 cacaggttgt cgcattgcaa caggatctta ttcataacgt aaagcctcag cgggttgtgt
    23641 ggcggcagcg cgccatgaag ggtaaagcgt agacagcagc gcgatagcca tcgccaccag
    23701 cgcaataaca atgacctgta aaggttcgat agccaccggc agcgccgcgc catcaagcag
    23761 gacgccgatt atcggcatca gattatttaa ctggctggca agcagtgcgc caagcgccgc
    23821 tccgaggatt gcaccgataa tcccggcgct ggccccttgc accataaaga ccatcatgat
    23881 ttgtcgcgga gttaagcctt gcgtttgcag gatcgcgact tcaccctgct tctccatcac
    23941 catcagcccc agcgaggtaa taatgttaaa cgccgcaaca gcgacaatca ggctcagcag
    24001 taagcccatc atattttttt ccatgcgtac ggcctggaac agctcgcctt tacggtcacg
    24061 ccagtcctgc catttgctgc cttcaggcag tttttgctga cttaaagagt caactttcag
    24121 cggctcatcc agccacaaac gccagccggt aatattggct gccggataac gcatcagacg
    24181 cgaggcatcc tcaatattca ccagcatttc atagccatcg acttcactgt tggcggcgaa
    24241 agtgccaatc acattgaaca ggcgctgact tggaatacgc cccatcggcg tgaactggct
    24301 ggcagatggc accatcacgc ggatttgatc accgcgattg acgcccaact gtgaggcaag
    24361 ttgttcgccg aggatgacat tatatttccc cggctcgagg tcagtttgtt tcacattgac
    24421 cagatacggc gttagtggat ctttttgcgc cgggtcgata ccgagcatca ccccgaccgc
    24481 cacgctgcgc gcgctttgca gtaccacatc accggtagta ataggtgcga cgcgattaac
    24541 gccgtccagt ttgactgccg tttccgggag ttgctgcggg ttaagagagc catgctcaga
    24601 agagagaatt gcctgtggca tcaggccaag gatgttgttt tgcagctcgc gctcaaagcc
    24661 gttcatcact gacaatactg tgaccagcgc catcaccccg agggtaatgc cgatggtaga
    24721 aagccaggag acgaaacgac cgaagcgatc cgctgcacgc ccacgcatgt aacgcaagcc
    24781 aataaataga gcgacaggtt ggtacatgaa atccgtctgg ttgctgtagc aaagacacga
    24841 gtatataagc gaaagcaaga tgggtaaacg tctgaactgt aagtgatcgt cagggctaat
    24901 ctgaaaagcc cccgtaaatc aatcggctaa attgccatgt tttatcatca atcttgccgc
    24961 ctgatcttaa tctgaaaaat catccaatac agacagatac ccattacacc tcgggtcata
    25021 accagcagga tcaaccgcat gtaaattaaa aaagagcgtg ttgttctgat gaaacaaaaa
    25081 gagctatgga ttaaccagat caaagggtta tgtatttgtc tggtggtgat ttatcactcg
    25141 gtcattacct tttatccgca tatgaccact tttcagcatc cgttatcgga agtcctgagc
    25201 aaatgctgga tctatttcaa tctttacctt gccccctttc gtatgccggt ttttttcttt
    25261 atatccggct atttgattcg ccgctatatc gacagcgtac cgtggggaaa ttgtctcgat
    25321 aaacggatct ggaacatctt ctgggtgctg gcgctttggg gcgtggtgca gtggctggca
    25381 cctgagcgcg atttaagcaa tgcctccaat gccgcttatg ccgattccac cggtgagttc
    25441 ctgcacggga tgatcaccgc cagcaccagc ttgtggtatc tgtatgcgtt aattgtctat
    25501 ttcgtggtat gtaaaatttt tagccgtctg gcgctgccac tattcgcctt gtttgtactg
    25561 ctgagtgtgg cggttaattt cgttcccacg ccgtggtggg gaatgaacag tgtgatacgc
    25621 aatttgcctt attacagcct tggcgcatgg tttggcgcaa caataatgac ctgtgttaaa
    25681 gaggtgccgt tgcgccgcca tctgctgatg gcttctttgc ttaccgttct ggcggtcggt
    25741 gcctggttgt ttactatctc gctgctgttg tcgctggtat cgactgtggt gatcatgaag
    25801 ctgttttatc agtacgagca acgattcggt atgcgctcca ccagcctgct gaatgtgatt
    25861 ggctccaaca ccattgctat ctacaccacc catcgcattc tggttgaaat attcagctta
    25921 actctgcttg cgcaaatgaa cgcagcacgc tggtcgccgc aagtcgaact gacactcctg
    25981 ctggtttatc cctttgtcag tttgttcatc tgtactgttg cgggcttgct ggtaagaaaa
    26041 ctttcacagc gtgcattcag cgatctgttg ttctccccgc cttcactgcc cgctgccgtc
    26101 agttactctc gttaaaccat caccccagcc ggggcgattc aatttgctaa accatgtcga
    26161 ttacggataa tgaacagcat tgcttatcag agatatgccc ccatatgttg aggcatatcc
    26221 taacgagaat ctgacaaccg ttatgcctga acaatatcgt tatacgctgc ccgtcaaagc
    26281 gggtgagcag cgtctgctgg gcgagttaac cggcgcagcc tgtgcaacgc tggtagcgga
    26341 aattgccgaa cgtcacgccg gtccggtggt actcattgca ccagatatgc aaaatgctct
    26401 gcgtttgcat gatgaaatca gccagttcac cgatcagatg gtgatgaatc tggcggactg
    26461 ggaaactctt ccctacgaca gtttttcgcc tcatcaggac attatctcct cgcgcctttc
    26521 caccctttac cagctaccga cgatgcagcg tggcgtactg attgttccgg tgaatacgct
    26581 tatgcagcgc gtttgcccac acagttttct ccacggtcat gcgctggtga tgaaaaaagg
    26641 tcagcgcctg tcacgagatg cattacgaac ccaactggac agcgccggtt atcgctatgt
    26701 tgaccaggtg atggagcacg gcgaatacgc cacgcgcggc gcgttgctgg atctcttccc
    26761 gatggggagt gagctgcctt atcgtcttga tttctttgat gatgaaatcg acagcctgcg
    26821 ggtgtttgac gtcgacagcc agcgcacgct ggaggaagta gaagcgatca atctgctgcc
    26881 cgcacacgaa tttccgaccg ataaagcggc aattgaactg ttccgcagcc agtggcgcga
    26941 taccttcgaa gtgaagcgcg atccggaaca tatttaccag caagtgagta aaggcacatt
    27001 acctgccggg atcgagtact ggcagccatt gttcttcagc gaaccactgc cgccgctgtt
    27061 cagttatttc cctgccaata ccttgctcgt gaatactggc gatctggaaa ccagtgccga
    27121 acgtttccag gctgacacgc tggcgcgttt tgagaatcgc ggcgtcgatc cgatgcgccc
    27181 gctgttgcca ccacaatcgc tctggctgcg ggtggacgag ctcttctcag agctgaaaaa
    27241 ctggccccga gtgcagctaa aaactgaaca tttaccgaca aaagccgcga atgccaattt
    27301 aggtttccag aaactgccag acctggccgt tcaggcgcaa caaaaagcgc cgctggatgc
    27361 gctgcgtaag ttcctcgaga ctttcgacgg tccggtggtg ttctcggtag aaagtgaagg
    27421 tcgccgtgaa gcgctgggtg aactgctcgc acgaattaaa attgctccgc aacgcattat
    27481 gcgtcttgat gaagccagcg accgtgggcg ttatctgatg attggcgctg ccgaacatgg
    27541 ttttgtcgat aaggtgcgta atctggcgct gatctgcgaa agcgatctgc tcagtgaacg
    27601 tgttgcccgc cgtcgtcagg attctcgccg caccatcaac cccgatacac tgatccgtaa
    27661 ccttgcggag ctgcatattg gtcagccggt ggtccatctg gagcacggcg tcggtcgcta
    27721 tgccggaatg accacgctgg aagctggcgg cattactggc gagtacctga tgctcaccta
    27781 tgccaacgac gccaaactgt atgttccggt gtcgtcactg catctgatta gccgttacgc
    27841 aggtggcgcg gaagaaaacg ccccgctgca taaacttggc ggcgatgcgt ggtcacgcgc
    27901 gcggcagaaa gcggcggaaa aagtgcgtga tgtggcggcg gaattgctgg atatctacgc
    27961 gcaacgcgcc gccaaagagg gcttcgcgtt taaacacgat cgtgagcagt atcagttgtt
    28021 ctgcgacagc ttcccgtttg aaaccacgcc ggatcaggcg caggccatta atgcggtact
    28081 taacgacatg tgtcagccgc tggcaatgga tcgtctggtg tgcggcgatg ttggctttgg
    28141 taaaacagaa gtggcgatgc gcgccgcttt cctggcagta gataaccaca agcaggtagc
    28201 ggtgttggtg cctaccaccc ttcttgcgca gcagcattac gacaacttcc gcgaccgttt
    28261 cgccaactgg ccggtacgta tcgaaatgat ctcccgtttc cgcagcgcca aagagcagac
    28321 gcaaatcctt gcggaagtgg cggaagggaa aatcgatatt ctgatcggta cgcacaaact
    28381 gctgcaaagt gacgtcaagt ttaaagattt aggcctgctg attgtcgatg aagaacaccg
    28441 cttcggggtg cgtcataaag agcgcattaa agcgatgcgc gcgaacgtgg atattctgac
    28501 gcttactgca acgccgatcc cacgtacgct gaatatggca atgagcggaa tgcgtgacct
    28561 gtcgattatc gccacgccgc ccgcccgtcg tctggcagtt aaaacctttg tccgtgagta
    28621 tgacagcctg gtggtccggg aggcgatcct gcgtgaaatt ttgcgcggag gacaggttta
    28681 ttatctctac aatgatgtgg aaaacattca gaaagccgcc gaacggctgg cagaactggt
    28741 gcctgaagca cggattgcca tcggtcacgg gcagatgcgc gagcgcgaac tggaacgggt
    28801 gatgaatgat ttccatcatc aacgtttcaa cgtgctggtt tgtaccacca ttatcgaaac
    28861 cgggatcgac atcccgacag ccaacaccat tatcattgaa cgtgcggatc acttcggtct
    28921 ggcgcagctg caccagttac gcggtcgcgt cggacgttca catcatcagg catatgcatg
    28981 gctgctgacg ccgcatccaa aagcgatgac taccgatgca caaaaacgtc ttgaagcgat
    29041 tgcctcgctg gaagatctcg gggcaggttt tgcgctggca acgcacgatc tggagattcg
    29101 cggcgcgggt gaactgcttg gcgaagaaca aagcggctca atggaaacca tcggtttctc
    29161 gctgtatatg gagttgctgg aaaacgccgt cgatgcactg aaagccggac gcgagccgtc
    29221 gctggaagat ctcaccagcc agcaaacaga agtcgagctg cggatgccgt cgctattgcc
    29281 agatgatttc atccctgacg tgaacacgcg tctgtcgttc tacaaacgta ttgccagcgc
    29341 caaaacggaa aacgaactgg aagagatcaa agtcgagctt atcgatcgct tcggcctgct
    29401 gccagatccg gcgcgtaccc tgctggatat tgcccgtctg cgccagcaag cgcagaaact
    29461 ggggatcagg aagctggaag gtaatgagaa aggcgggatg atcgaatttg ccgagaagaa
    29521 tcacgttaat ccggcctggt tgattggttt gctgcaaaaa cagccgcagc attaccgcct
    29581 tgatggtccg acgcgcctga aatttattca ggatttgagt gagcggaaaa cgcgtatcga
    29641 atgggtacgc cagtttatgc gtgaactgga agagaacgcg atcgcttaat gtaaatcggg
    29701 ggcgtttctg cgcccccatc tgcaacattt acaaattctt tgcactttcc tgcactatcc
    29761 gacactgtca ccatccataa ttcaggctta tctgtttatt acaataacct tatatttatt
    29821 atggattttt ggtgatgatc aaaacgcgtt tttctcgctg gctaacgttt tttacgttcg
    29881 ccgctgccgt ggcgctggcg ctaccggcaa aagccaacac ctggccgctg ccgccagcga
    29941 gcagtcgtct ggttggcgaa aacaaatttc atgtggtgga aaatgacggt ggttctctgg
    30001 aagccatcgc caaaaaatat aacgtcggct ttctcgctct gttacaggct aaccccggcg
    30061 ttgatcctta cgtaccgcgc gcgggcagcg tgttaacgat cccgttgcca accctacttc
    30121 cagacgcgcc gcgcgaaggc attgtgatca acattgcgga gctgcgtctc tattactacc
    30181 cgccgggtaa aaattcggta accgtgtatc caataggtat tggtcagtta ggtggtgaca
    30241 cgctgacacc gacaatggtg accaccgttt cagacaaacg tgcaaaccca acctggacgc
    30301 caacggcaaa catccgcgcc cgttataaag cacagggaat tgagttgcct gcggtagtgc
    30361 cggctggacc ggataaccca atgggccatc atgcgattcg tctggcggcc tatggcggcg
    30421 tttatttgct tcacggtacg aacgccgatt tcggcattgg catgcgggta agttctggct
    30481 gtattcgtct gcgggatgac gatatcaaaa cactctttag ctaggtcacc ccaggcacca
    30541 aagtgaatat catcaacact ccgataaaag tctctgccga accaaacggt gcgcgtctgg
    30601 ttgaagtaca tcagccgctg tcagagaaga ttgatgacga tccgcagctg ctgccaatta
    30661 cgctgaatag cgcaatgcaa tcatttaaag atgcagcaca aactgacgct gaagtgatgc
    30721 aacatgtgat ggatgtccgt tccgggatgc cggtggatgt ccgccgtcat caagtgagcc
    30781 cacaaactct gtaaatcctg gataaaaaaa atccccgcga cacggcatgt cgcggggaaa
    30841 aaatcagcag tagcaggcat taatgagggt taatgcttat ttataaatta ctgctgttcc
    30901 atggagggta ttcggaccgg ttacagaagt aatacggaaa gattttgcgc ccatctcatc
    30961 cgctttttgc gccagctgct cttccagcga tcccagattt gtccccgcgt tagcactgat
    31021 agtaccgact ttttgttggc cttctggcgt tgactgaact tcgacagccg caaagttggc
    31081 aaatgacatg gagcttaaaa tcgccgcagc gatgagggtt tttacgtttt tcataatagt
    31141 ggccttatgc agatgaatga cgtcgcaaga aattacttaa cgatcgataa ctaaatgata
    31201 gatgtgatct ggatcacata caagatattt tttataacaa tcattaatta atttaaaaat
    31261 ctttaaattt cagtcaaata gaaagcaatt atttttatgg aatctggact ggtgaacgga
    31321 ggtgattatt tttataataa cctttcattc acagtcacca ggtacaacgg catcatggca
    31381 actgactcaa cacaatgtgt aaaaaaaagc cgtggccgcc caaaagtgtt cgacagggat
    31441 gccgcgcttg ataaggccat gaaattgttc tggcaacacg gttatgaagc gacttctctt
    31501 gcggacctcg tcgaagcgac cggagccaaa gcgcccacgc tatacgcgga atttaccaac
    31561 aaagaggggt tatttcgtgc cgttctcgac cgctatatcg atcgttttgc cgctaagcat
    31621 gaagcacagc tgttttgtga agagaaaagc gtggagtctg cgctggctga ctattttgct
    31681 gccatcgcca actgctttac cagcaaagac accccggcag gctgcttcat gatcaacaac
    31741 tgcaccaccc tctccccaga ttcaggagat atcgccaata cgttgaaatc acgccatgcg
    31801 atgcaagagc gcactttgca gcagttttta tgtcaacgac aagcgcgcgg ggaaatcccg
    31861 acccactgtg acgtgacaca tctggcagaa ttccttaatt gtattattca ggggatgtcg
    31921 atcagcgcac gcgaaggtgc atcgctggaa aaactgatgc agattgccgg aacgactttg
    31981 cgtttatggc ccgaactggt gaaataagtc gcaaaaaaag cccctcagcc tgagcgaatg
    32041 aggggccaaa ttgcagagta cagccttgtt atactttgtt attcaaaatc agttgcccgt
    32101 tactatctag cgggatctgc gtacccggat cgcggtccat gcggattttg ccctgctgat
    32161 cgccaatctt ataggtcaca tcataaccga gcattttttc tgacttgtca tacaccgttt
    32221 tacaacgctg ttgcgtagtc gtgtaagtat cgctttcctg gagagagccc tggatctggt
    32281 taccggcata tccaccaccc agcgccccca caacagtggc gacatcttta ccgcgaccac
    32341 caccaaactg atgccctatc acgccaccag caacagcgcc gagcaccgac ccggtaatgc
    32401 gattttcatc ctgcaccggt cgacgatggg tcactgtgac gttgcgacac tcctgacgcg
    32461 gtgttttaac cgtttccttg attggggttg cagaaacaac ctgagcgtat tgcgggcccc
    32521 gttcaaacac gttcagactg gccactgccg ctacgcccaa cgcagctgcg acaccaatcc
    32581 cgatacccgc caacattgat ttattcacgg gaacacctcc ttctttgcag ttatgccaat
    32641 catgcaagcg aaggagaaaa gcagctatca gacagaagat gaaaagcgcg ggaatgactg
    32701 gaaatactta aggattcgga ggagtctgaa ggaatgcctg atgcgacgct taacgcgtct
    32761 tatcaggcct accgctcgta ccatttgtcc agggcggata aggcgtttat gccacatcag
    32821 ccagtgtacg tcgattaatg caacttcaaa cgcggacgga taacgcggtt aatactcccc
    32881 accagcatca ttaatccggt tttaaagtaa ccatgcagcg caatctgatg cattcggtat
    32941 agcgagatat atacaaagcg cgcaattcgt ccttcaatca tcattgagcc gcgcgtcagg
    33001 ttacccatca ggctaccaac ggtggagaag ttcgacagcg ataccagcga accatgatct
    33061 ttatactgat aatttttcag cggcttaccg ttcatctgcg ccagaatgtt gttcattgcg
    33121 caagtcgcca tctggtgtgc agcctgagca cgcggcggaa caaagccccc ttccggacgc
    33181 gggcatgacg cgcagtcgcc aatagcgtaa atgtctggat cgcgagtggt ttgcagcgtc
    33241 ggttccacca ccagctggtt gatacggtta gtttcaagac caccgatatc tttcaggaag
    33301 tctggcgctt tgatcccggc tgcccatacc atcagatcag cctcaatata ttcgccatct
    33361 ttagtgtgca ggccgccttc atcagcactg gtgaccatgg tttgcgtcag cacgcgaacg
    33421 ccaagtttcg ttagctcgtt gtgggccgca gcagagatac gtggcggtaa tgcaggcaaa
    33481 atacgttctc ccgcttctac cagcgttacg ttcagggctt cgttggtcag gcctttgtaa
    33541 ccgtagctgt gcagttgctt gatcgcgttg tgcaattcag cggagagttc tacacccgtc
    33601 gcgccgccgc cgacaatcgc aatgttcact ttgccatttg cgcccaggtt ggcagagtat
    33661 ttcaggaaca gattcagcat ctcctggtgg aagcgacgcg cctggtgcgg gttatcgagg
    33721 aaaatgcagt tctctttgac gcctggcgta ttgaaatcgt tagaggtgct acccagcgcc
    33781 atgaccaggg tgtcataggc gattttacgt tccggaacca acagttcacc tttctcgtcg
    33841 cgtagttctg caatagtgat tgttttcgct tcacgatcaa tatcaatgac ggaacccaac
    33901 tggaactgga aaccatgatt gcgcgcatgg gccagatagc tcaacgcatc gacgccttca
    33961 tcaagcgagc cagtcgccac ttcgtgcagc agtggtttcc acaggtggct gtggttacga
    34021 tcgaccagcg taattttggc ttttttctta cgtcccagct tatgccccag ctgtgttgcc
    34081 atttccagcc caccagcacc gccgccgaca atcacaatct ttttcaatgg cgtagtcaac
    34141 gtgaccccct taaatttatt aaccaattgt taattaatgg ttattaacat agctcttaat
    34201 taacaacagg ttacagcaat gaaaatgttc ttcgctattg agaataacat gaatggtgca
    34261 ttggtcatac caaaattgat gttaatcaag ttttgttgct taaaagatag gcaactttac
    34321 cgggggagat caaaaaatag cgtttattat ttgagcaaat tcggaagagt aatatagggt
    34381 gaagcgtata tcgagggcgt ttggtcgtcc tgataagatg cgcagcatcg catcaggcat
    34441 tgatgctcga ctgtcggatg cggcgtggac gccttatccg acttacagaa ttcggagcgc
    34501 atcaggtacc ggcaaggtta cctgatgcga cgagggcatt tacccgaggg ttttgaacgc
    34561 tttaatgcgc tgtaaatgcg gggagatatt cttgaatttg tgcgtctgct cttcgtccca
    34621 gacaatctcg taataatgat gcaactcttc agatgtccgc tggctgttaa gtgcttcatc
    34681 attacgcgac aaaataacca gacaacgatc gcgattcttc tcgcggaagt tggtcacaca
    34741 cttagtggca atatcggcat actcttccgg gcgatcaatc ttgccttcca tgttctcata
    34801 agggaacaaa ttagggttga agatcacctg gcggatatcg caaagaaaac caatccgttc
    34861 cgcccagtat ccgcccaggc caacgccgca aattagcgga cgctcgtcaa cgttcagttg
    34921 caacattttg tccacttctt taagcagatg ctgcatatca tgtttcggat gccgcgtact
    34981 gtagcttatc aagcgtacat ccgggtcaat aaactgcaat tgtaagactt tctcgtggtt
    35041 acccggactg ttagagtcaa aaccgtgtaa atagataatc atcgcttcct cacataagcc
    35101 agggttagtg acctgctttc tcttcctgcc agcgttcatg taactgattc agacgggtgc
    35161 tgatcgcttt ccagcgagcc gagtccatca gttcctgtcg cgaaaatgaa cctttatgat
    35221 acaaacgtgt aacacgttct gccttgatcg gtgacagatt atctaacacg ctgacggccc
    35281 ctttacgatt attgcagacc aggatcatat cgcaacccgc atccagtgat gcctgcccgc
    35341 gttcggcata actgcccata atcgcggcac cttccatcga taaatcgtca gagaaaatca
    35401 cgccgtcaaa acccagttcc tgacgcaaaa cggttttcag ccagtaggga gaaccgctcg
    35461 ccggacgcgg atcaacatca ctgtagatca catgcgcagg cataatggcg tcgagtttat
    35521 tttcgcggat taaggaactg aagaccgaca tatctttagc gcgaatctcc gcttgtggac
    35581 gtggatcgca cggtgtttct ttgtgtgagt ctgccgttac tgcaccgtgt cctgggaagt
    35641 gtttcccggt cgttttcatt ccggcttcat gcataccatc aataaaccgg ctggcaattg
    35701 ccagggcttt ttgtggatcg gcatgataag aacgctcgcc aatcgccgcg ctgatatgcc
    35761 cgacatccag cactggcgca aagctgatat caatatccat agcgatcatt tcgctggcca
    35821 tcagccaacc cgcctcttgc gccagtttgc cgccctcttc cattcccaac agcgcagcaa
    35881 aggattgtgc tgccggtaag cgggtaaaac cttcgcgaaa acgctgcacg cgtccacctt
    35941 cctgatctac cgccaccacc agatgattgc gcgatgctgc gcggatctgg cgcaccagtt
    36001 cacgtaactg ggcaggatca tgatagttac gcgtaaagag aatcagccct cccaccagcg
    36061 gatgcgccag tatttcacgc tcttccgcgt ccagttcgta accttcgaca tccaacatta
    36121 ctggacccac actgctctcc ttattgtttt attaatagct gccgccaggt gtcatcggcc
    36181 agcctgataa attgttgatc gccggtttgt cgccagcggt actcaaacca ccctcctttg
    36241 agcatcagca gccagggaaa ccatcgcctg acctgacgcc ataattgcgc cggataaatc
    36301 ttcgcgcgag tggcatagtc attgaccaat tgccggtgct gttcagtatt ttccacccac
    36361 accgccgcca gttccagcgc gatatcacca tctccagcat actcccagtc gatgagtttt
    36421 aaccctgatg cgctatgcac taaatttccg gcgtggacat ccatatgcaa tggacttaag
    36481 cgtaaaggtc gtggttcccg cgctttgcgc agacgtttta acattcgcag ccaacccaca
    36541 gtccgccgcg ccggatcgct ttgctgccag tacagttcca gtaagggcaa cagcgttatt
    36601 cgccagccaa aacgtggttg ttgatgtaga taatacagca agcctgccag ttcgttggta
    36661 tccggcaaat acgtttttac cgcgccgggc agatagtcga ctaccatcca gtcacggaga
    36721 tataaatgcg gcttcggtgc aatgcttgcg ggtagttgtg ataaagcccg atactggcgc
    36781 aagaacgcgg actgcggcgc atcaggatcg tgcggctgac gcacaacaaa acgctggccc
    36841 tgatgttcaa tgagaaaact accgccacta agcccactat taaacgtcgt gacgggatga
    36901 aactgcggga aaaagcgcga cagcaattcg tcgcgcgtga tgggattatt gctgcgaaac
    36961 ggcaccttta cctgaccaga taatttcgcc cgtctgcacc agcatcagct gcatttgtag
    37021 ggtcggagcg ttaacgttgc cagaggcgct ggagtacagc acgtaatgag cgccgacatt
    37081 gcgggcaatg cctatggctt tactacgggt gcctaaactg tcctgcggcg acaaacctaa
    37141 ctgttgctta gccatcgaca gttgctgggc ggaaaccagg gtaaatttcc cgttattagc
    37201 cagcgcattt cgcagcgttt cggtcgcttc tgcggcattc agcgaaccgt tagtacggtt
    37261 gttaacgcta tcaaccagca ggacgctacc cgcagtcacc ccgtcagccc caagcatctt
    37321 actgaccatc ggctgcattg cgccattcca gtcataatgg cgaatatgcg gcgcaggcgg
    37381 tgccgttcga tcttcgtgct caattgggcc tggctgctgc gggatcgtcg gcaccgaggg
    37441 cactgtgggg acaggctgtt gtggctcggc tggttgttcc ggcgctggtt tcacttcttc
    37501 taccggtgca ggttcacgtt gccccacaca cccggcgaga aacatcgcca gcgcggtaat
    37561 caaggcgtag cgactcattt ttgtcatcaa gattcacccc tttacaaata tagataaagt
    37621 ctgactttgt gcgcccccag aaaattggcg ctgccgtaca gcgttaccgc cgaatgtgcg
    37681 ggaatggtaa cgctgcgtgg cctttccaga ggatgcatct ccagccctct ggcgtcatac
    37741 cagtaaaaac gataatgaac ggtaacgggt tcttgccttt cgttataaag cgttgaggat
    37801 gctgaaggtt gaatatcaga cgtcgaaagg acgggctttt ctgcactgat gcccgcagcc
    37861 aataaagatg actccatcac cagcgattgc tcatcattca ccggaatttc cggatgtgaa
    37921 cgacagccca ccagcagtaa caacaccaga gacaccagcc caaagcatcc ttttctcatc
    37981 gttacagacc tttatgcgcc agcattggtc ccagcggacg gccacccaac aagtgcatat
    38041 ggatgtggta aacctcttgt ccgccatggc ggttggtatt catgatcaga cgatagccat
    38101 cttcggcaat accttcttgc tcagcaattt ttgccgctac ggtgatcatg cgtcccagcg
    38161 cctgctcatg ctcagctgac acgtcgttca cagtcggaat gaggatattc ggaatgatca
    38221 gaatatgcgt tggcgcttgc ggcgaaatat cgcgaaacgc cgttaccaga tcatcctggt
    38281 agacgatatc ggaggggatc tcacgacgaa taattttgct gaatatagtt tcttctgcca
    38341 cgacattttc ctttttcaaa tagccatgcg ttgcgccatg ctacgccgcg ccacactgcg
    38401 agtatagagt atggtcgagt taaacctgct ctttcaacct taactcacga tttcttaagc
    38461 aaaaaatcgt tttgctgccg tatatatcgc cattattccc atttctgcct gcgaatgcgc
    38521 tgaaaagaca ttctctgttt ggtttacacg acaaatatga atgcgtatat ttctcatttg
    38581 catttgcaag caaaattatt cgcacactaa atataaccgc tcatcaccct cctccggcat
    38641 gagcctgacg acaacataaa ccaagagatt tcaaatgctt tcaacacaat ttaacaggga
    38701 taatcaatat caagccatca ccaaaccgtc actacttgcc ggttgcatag cactggcact
    38761 attaccttct gccgcttttg ctgcaccagc cactgaagaa acggtgattg ttgagggttc
    38821 agccacagct ccagatgatg gcgaaaatga ttacagcgta acgtctacct ctgcgggtac
    38881 caaaatgcag atgactcaac gtgatattcc tcagtcggtc actattgtta gccagcagcg
    38941 gatggaagat cagcagttac aaacgctggg cgaagtgatg gaaaacacgc tggggatcag
    39001 caaaagtcag gcggattccg atcgtgctct ttattattcc cgcggattcc agatcgataa
    39061 ctatatggtt gatggtatcc ccacctattt tgaatcgcgc tggaatctgg gcgacgcact
    39121 ttctgatatg gcactgtttg aacgcgtaga agtagtgcgt ggcgcgacag gactcatgac
    39181 cgggacgggt aatccatctg cggcaattaa tatggttcga aaacacgcga ccagtcgtga
    39241 atttaaaggc gatgtctcgg cggaatacgg tagctggaac aaagaacggt atgtggcgga
    39301 tttacaaagc ccactcaccg aagacggtaa aatccgcgcg cgaattgtcg gcggctacca
    39361 gaataacgac tcatggctgg accgctacaa tagtgaaaag accttcttct cgggcattgt
    39421 cgatgctgat ttaggcgatc ttacgacgct ttcagccggt tacgaatatc agcgcattga
    39481 tgttaatagc ccaacctagg gcggtttacc gcgctggaat actgatggca gcagcaacag
    39541 ttacgatcgc gcacacagta ccgcacctga ctgggcgtac aacgataaag agatcaacaa
    39601 ggtctttatg accctgaagc agcggtttgc tgatacctgg caagcgacac tgaatgccac
    39661 ccactctgaa gtcgaatttg acagcaaaat gatgtatgtc gatgcctatg taaacaaagc
    39721 ggatggtatg ctggttgggc catacagtaa ttatggacct ggctttgatc atgtcggcgg
    39781 caccggttgg aacagtggca aacgtaaagt tgatgcgctg gatttgttcg ctgacggtag
    39841 ttatgaattg tttggtcgtc agcacaatct aatgtttggt ggcagttaca gcaaacaaaa
    39901 caatcgttac ttcagttcat gggccaacat cttcccggat gaaattggca gtttctacaa
    39961 ctttaatggc aatttcccac aaaccgactg gtcaccacag agcctggcgc aggacgatac
    40021 cacacatatg aaatcgttat atgctgccac tcgtgtcacc cttgccgatc cgctgcatct
    40081 gatcctcggc gcacgttata ccaactggcg ggttgatacg ctgacttaca gcatggagaa
    40141 aaaccacacc acgccttacg ctggtctggt gtttgacatc aatgacaact ggtcgaccta
    40201 cgccagctat acctctattt tccagccgca aaatgatcgt gacagttcag gcaaatatct
    40261 ggctccaatc accggtaaca actacgagct gggtctgaaa tcggactgga tgaatagccg
    40321 tctgaccacc acgttagcca tcttccgtat tgagcaggat aatgtcgctc agtccaccgg
    40381 tacacctatc cccggcagca acggcgaaac cgcctataaa gcggtggatg ggacagtcag
    40441 taaaggggtg gaatttgaac tcaacggcgc aattaccgac aactagcagc tgacatttgg
    40501 cgcaacgcgc tatcttgcag aggataacga aggaaacgcc gttaatccta atctgccacg
    40561 caccacggtt aaaatgttca ccagctatcg gttgcctgtc atgccagagt tgacagtcgg
    40621 cggtggtgtt aactggcaaa atcgcgtgta taccgacacc gtgacaccgt atggcacctt
    40681 ccgcgccgag caaggtagct acgcgctggt ggatctcttc acccgctacc aggtgacgaa
    40741 aaacttctcg ttacagggga acgtcaataa cctgttcgac aaaacctacg ataccaacat
    40801 ggaaggttct atcgtctacg gcgcaccgcg taatttcagc attaccggca cgtatcaatt
    40861 ctgaataaca cctgtaaaaa aggcagccat ctggctgcct tagtctcccc aacgtcttac
    40921 ggattagtgg ttacggatgt actcatccat ctcggttttc aggttatcgg atttagtacc
    40981 gaaaatcgcc tgaacaccag aaccagcaac cactacgccc gctgcgccca gtttcttcag
    41041 gccagcctga tccactttag acacatcagc aacgctgacg cgcagacggg taatacatgc
    41101 gtcgaggtta gtaatgtttt ctttaccacc aaatgcagca accagagccg gtgccatttc
    41161 gctggtacct gttgcttttg catcttcagt cgcgtcttca cgacccggcg ttttcagatc
    41221 cagtgcttta atcagcacgc ggaagatggt gtagtaaaca atcgcataac cgataccgac
    41281 gatcgggaac agccacagtt tgctgctgtt accagacaga acgatgaagt cgatcagacc
    41341 gtgcgagaac gacgtaccgt cacgcatccc cagaagaata cagattggga atgccaggcc
    41401 tgccagaatc gcgtggatga tgtacaggat cggcgcaacg aacatgaaga agaactcgat
    41461 cggctcggtg ataccggtca ggaacgaggt cagcgccgcg gagatcataa taccgcccac
    41521 tttcgcgcgg ttttctggtt tagcagagtg ccagatagca attgcggcag ctggcagacc
    41581 gtacattttg aacaggaagc caccagacag tttacccgca gtcgggtcac ccgccatata
    41641 acgcggaatg tcgccgtgga aaacctgacc tgctgcgttg gtgtattcac caatctgcat
    41701 ctggaaaggt acgttccaga tgtggtgcag accaaacggt accagacaac gttcgatgaa
    41761 accgtaaatg ccaaacgcaa ctaccgggtt ctggtaagca gcccactgag agaaggtctg
    41821 gattgcagaa ccaatcggcg gccaaatgaa ggacagcaca acgccagtaa agatggcagc
    41881 caggccagaa atgatcggca caaagcgttt accggcaaag aagccaagat actcaggcag
    41941 cttaatacgg tagaaacggt taaacatgta cgctgcaatc gcaccggaga taatccctcc
    42001 gagtacgcca gtatccgcca agtgtttaga ggcgatttct tcagcaggta aatgcagtac
    42061 cagtggcgca accacggcca tggttttaac catgatgcca taggcaacaa ctgcggccag
    42121 cgcggatacg ccatcgttat tggtaaagcc gagggcgaca ccgatcgcaa aaatcagtgg
    42181 catgtttgca aagacggaac cgcctgcttc tgccataaca tgcgatacaa cggcgggcag
    42241 ccagctgaaa ttcgcggaac cgacgcccag cagaatacct gcgataggta gtacggatac
    42301 cggcagcatc agcgatttac cgaccttttg caggttagca aatgcattct taaacataat
    42361 tgagagtgct cctgagtata ggtgcttttt ttacgttctc acgcgtggca aggggggaga
    42421 gcctcgccgt gtacagggca tctaagcgcc ctttatttat tacacagagt aaaataattc
    42481 agtgccaatt tgtttgacgg ctatcacgtt tcaacttcag atcattggag aagttgcaca
    42541 ttttgacaaa acatatgtca caatatttca aacgcggtta ttcaccgccc aattttcggc
    42601 ggtgaacttt actcgtttta gccattaatt acgagcttta aaaaaactca ttcaacggat
    42661 ggattgaagg cgggaagcgt cgatgtgaaa cagacgggcg aagttatcgg tggttacctg
    42721 cgccagttct tcaacggcaa cacctttcaa cacagccatg tattctgcaa cgtcacgaac
    42781 catcgcaggt tgattctctt ttcctcgatg cggtaccggc gcaaggtaag gtgagtcagt
    42841 ttccaccagt aatcgatcca gggggacata acgcgcagca tcgcacagtt gctccgcatt
    42901 acggaaggtc acaatgccgg aaaaggagat gtaaaatccg agatccagta atttacccgc
    42961 cgtttctctg tcctctgtaa aacagtgtag tacgccaccg caatccgtca ctttttcttc
    43021 gcgcagaatt gccagcgtat cggcacgggc gtcacgggta tggacgataa ccggcttgtt
    43081 cagttcacga ccaatctgga tatgatggat gaaggactct tgctgacgta ctttagtttc
    43141 cggtgtgtaa taataatcca gcccggtttc acccagcgct acaacaccct cttctgccgc
    43201 cagacggcgt aaatcttcta catcgtaggg atcattctgg ttgagtggat gcacgccaca
    43261 agaaaatacg acgttgtcac gttcgcctac cagatcccgc atatgtaaat aacccggtaa
    43321 tgttgtggcg actgccagac aaaatttcac atcgcgtgcg gcggctttcg ccagaacgtc
    43381 atccacgtcc ttatgcaaag attcataatc cagaccatcg agatggcagt gtgagtcgac
    43441 taaaaacatg atgtctctct taaagatgag gaaccggtag cacaacgccc ggttgcaggt
    43501 aatgctcaat acgcagtaaa agatcggtga tgagaagctc gcggttgatg cctgtgacag
    43561 acattaactg ttcacgaatg tggcaaacat cccccagtat agcctgcagg cgcgagggag
    43621 aaagatggtt tgccagttcg gcgaccaggc ccggcacatc aacattggtc acctgcgcag
    43681 cgccatgatg gcgttttagc gcatccatca gcaacgttgc cacccagtgt aaacgcgccg
    43741 gagcttgttc atgattaagg gccgccagca gcgaatacca gtcgcccgat ggcacgctat
    43801 atgccaacgc ctgacacaat gtttcacgag cctgccagtt atctccctga aacaacgcca
    43861 gtgccgcgcc aggcgaaccg gcgcttaagc gcaatgcggc aagtaatgca tcctgtgaca
    43921 ttgtcacttc gcgtgaaagc caagtcacgg cgtactgttc tggaggcggc gcaaggtaat
    43981 gtaaccgaca acgactacgt aatgttgcca gtaaacgttc aggctcgcgg gtagccagga
    44041 aaaaccaggt ttctgctggt ggctcttcaa gcgttttcag caatgcgtta gccgcggcgt
    44101 cggtcagtaa ggcagcatcg gttacccaga caactttcgc accacctagg cgtgcgtgct
    44161 cattcagctt ttcggtgacc tcacgtaccg catcaatgcc cagcgtattt ttcccttttt
    44221 cgggagccag ggtgtagtaa tcgggatgcg tgccagcctg catcaactga catccacgac
    44281 agtgaccgca acttttgtgg ccctgcggtt gttggcagag taaataacgg ctcagggcgt
    44341 agattaaagc atcatcgccc atgcccggta acgcctgaat gagtagcgca tggtgacctc
    44401 ttccggcctg atagctggct accagttttt cgaaatcagg tcgtaaccat ggataccatc
    44461 tcatgcgtcc aactccttca cccagtgggt cacggtagtg cggattgcat ccatcacggc
    44521 ctccagcggc tgggtagcat caatggtatg aatactttta tcttgtgcag ccagttccag
    44581 atagcgggcg cgggtgcgat taaagaaatc gaaagactct tgctcaatac gatccagctc
    44641 gccgcgcgta cgcgcgcgtt ttaagccaac ttccggggta acatcgagat agagcgttaa
    44701 gtcggggcga aaatccccga gaacagcatc acgcagtgtt gccagcatat gttggtcaat
    44761 accacgtcct ccgccctgat acgcctgagt ggagagatcg tggcgatcgc caatcaccca
    44821 ggtgccgtta gccagcgcag gtttgatgac cgtttctacc agttgaacgc gcgcggcata
    44881 aaacatcaga acttcggctt tatcggtaat gacttcatcg cctaccgatt tgatatccag
    44941 caccaggctt cttaactttt cggcaagttg cgtaccgcca ggttcccgag tgaaaaccat
    45001 gtcgcggata cccagttgct cgagcgtctc aaccaccaca ttacgcgcgg tagttttgcc
    45061 tgcgccttcc agcccctcaa tgacgatata cttactgcgc atttttttcc ttaagcactt
    45121 tcagataatc ctgcacagac ttgttatgac tggcaagatt ggtattaaac gtgtgaccac
    45181 ctttaccatc ggccacaaaa tagagatacg gcgtttttgc cggatgcgca gcagccttca
    45241 gcgaatccgc ccccggcgta gctatcgcac ccggcggcaa accggtaatg gtataggtgt
    45301 tatacgctgt cggcgtttcc aggtctgcac gagaaagttt gccattataa cgctctccca
    45361 tcccgtaaat cacggttggg tcggtctgca ggcgcatacc aatgcgtaaa cggttgataa
    45421 ataccgaggc aacctgatcg cgttcactgg caacggcggt ttctttttcg ataattgatg
    45481 ccatcgtcac cagctgattt ttatctttat aaggcagacc gtccgcacgc ccttcccagg
    45541 cgctatcgac cgctttcacc attttcttgt gagctcgctt gagtaacgcg acatcggtgg
    45601 tattggcggt atacatcaag gtgtctggcc agaaccaacc ttcaatccac tccgggtttt
    45661 ccagttcaag tgcctgcgct acggtggcgt acttatcgtc actcagcgtg tgcttgatat
    45721 acggagcctc acgcaattgc ttgaggtagt cgctcagacg catcccttct accagtcgca
    45781 gcgggaactg tgcttcttta ccgctttcca acaatttcag catctcgcgc acggtcatct
    45841 gcggtgtaaa gcggtaagtc ccggctttaa agtgagaaag atccggttcg atacgcagca
    45901 gccattgaaa aacccgtggg cgattgatga tcttatcagc ataaagctgt tcaccgagcg
    45961 ccagacgtcc ggtccctggc ttcagggtaa atatcgtctc ttctttgata agcaatttgc
    46021 tgtcggcaag atggcgaacc ttccagacgc ccacaccagc ggcgataccc agtaccacca
    46081 gcaataacaa gataatcaat aacacttttt tcatgactaa ttcgggcgct cacaaagtgg
    46141 ggctaaatat tcatataacg ttgctgacga aaaggtgaca tcgccacagg cacgtacggg
    46201 catcactggc attaacgcat tacaaataac catctcatct gcctgcaacg cctcctccag
    46261 cgaggcttgc acttcgacaa gctgataaga ggattgtgcc agtaaacgga tacagaattg
    46321 tcgcataatg ccgttaacac ctgcctgatc cagtcgcggc gtataaacta cattgccctt
    46381 ccgccagaac aaattagccg cacagcattc cgtaacccac ccttcgctgt caaggaccag
    46441 cgcctcatca gcgtttgtct gctcaagatg agagcgaatc aatacttgct caagccgatt
    46501 aagatgttta atacctgcaa gatgaggatt gcgccccagc cgcaccgggc ttagcgccaa
    46561 cgtcatccct tcgttacgca aacggtcgta atgtgcagga taagccgtaa cggagagaat
    46621 ccgcgttgct ggtccgctgt tcaatgtgct gtaccctcgc ccgccactac cgcgactgat
    46681 cacgactttc agcacaccat tttgctgttc tgctgccagc gttttcatct cttgttcaag
    46741 ctgaggccag aagtcacagg aaatcatcaa ccgctgacaa gcatcctgta gtcgctggat
    46801 atgcgccgat aacaaactga ctttaccgtc gataactctg gcggtggtaa aacaaccatc
    46861 accaaactgc gttgcccgat cgcttactgc cagcgattcc tgcttataac cgttaattaa
    46921 gaacataccg gctccttatc agagaattgt tagtgtggca gcatgttcac tacggaacaa
    46981 gtcggaataa aagctaagaa aaaaggcccg caagcggacc ttttatatgg gtggaaaatg
    47041 acaacttaga tctttttaaa gatcaaagaa ccattagtgc caccgaagcc gaaggagtta
    47101 cacagcgtgt attccattcc gctgacctga cgtgcttcat gcggtacgaa atccagatcg
    47161 caaccttcat ccgggttatc caggttgatg gttggcggaa cagcctgatc gcgcagcgcc
    47221 aggatggagt agatagattc tactgcaccc gccgcaccta acaggtgacc ggtcatagat
    47281 ttcgtggagc ttaccaacac acggcttgca gcttcaccga agatggtttt caccgcctgc
    47341 gcttcagctt tatcgccagc cggcgtagaa gtaccgtgcg cgttaacgta gccaatctga
    47401 ctcgcttcaa tgcctgcatc acgcagagca tttgccattg ccagagctgc gcctgcgcca
    47461 ttttctggcg gtgacgtcat atgataagca tcgctgctca taccaaagcc gacgagttca
    47521 gcgtaaattt tcgcaccgcg ttttttcgcg tgttcgtact cttcaagtac cagcataccg
    47581 gcaccatcgc ccagtacgaa gccatcacgc tctttatccc acgggcggct cgccgcttgc
    47641 gggttatcat tgcgggtaga taatgcacgt gccgcgccaa aaccaccaac gcccagcggc
    47701 gtactggctt tctctgcgcc acctgcaacc atcacgtcag catcgccata cgcgataata
    47761 cgcgcagcat ggccaatgtt gtgcacgccg gaagtacagg cggtcgcgat agagatgctt
    47821 gggccacgca ggccatacat gatagtcaga tgacctgcca ccatgttcac aatcgttgac
    47881 ggaacgaaga atgggctgat cttacgtgga ccaccgttca tcagagatgt gtggttttct
    47941 tcgatcagtc cgaggccgcc aatcccggag ccaattgcgg caccaatgcg ggttgcgttc
    48001 tcttccgtta tttcaaggcc agaatcctgc atggcctgaa cgccagcgac aattccatat
    48061 tgaatgaagg catccatctt gcgctgttct ttgcgcgaga taatgtcctc acagttaaaa
    48121 tcctttacta agccagcaaa tttcgttgca taggcgctag tatcgaaatg gtcgattagg
    48181 ctgatgccac tctgaccggc aagcagagct ttccaggtag actctacggt attgccgaca
    48241 ggagacaaca tgcccagtcc ggtcacaact acacgacgct tagacacgtt tgtcctccaa
    48301 ggagggaaaa atgattctag tgggacaaaa agataaaact caggcggtcg aacgaccgcc
    48361 tggagatgtt cacttacgcc tggtggccgt tgatgtaatc aatggcagcc tgaacggtgg
    48421 tgattttctc agcttcttcg tccggaatct cagtatcaaa ctcttcttcc agagccatta
    48481 ccagctcaac ggtgtcaaga gaatccgcgc ccaggtcttc aacgaaagaa gcattgttgg
    48541 taacttcttc ctgcttaacg cccagctgtt cgccgataat tttcttaacg cgttcttcga
    48601 tagtgctcat actcttaaat ttcctatcaa aactcgcttt cgcgatggtt ttcgtagtgt
    48661 ataaaatgtt gaaaaatttg caactaaatc ccggcaggtc ttaccacgat tttacgttat
    48721 tttgaggcct taccccccaa taacgcaaat cattttgcac taattattac tcataaccac
    48781 gcagactgcg cgcaacatga gcattttgtg caaatcgcgg tcagaccatg tacatcccgc
    48841 cgttcacatg caaagtttca cccgtgatgt aagctgcttc gtcggatgcc aggaatgcaa
    48901 ccgcgttggc gatttcctgt gcgccgccga ggcgacccgc aggaacctgc gccaggatac
    48961 ccgcacgctg gtcatcgctc agcgcacgtg tcatgtccgt ttcaataaag cccggagcaa
    49021 caacgtttac agtaataccg cgtgacgcaa cttcgcgcgc cagtgattta ctgaagccga
    49081 tcaagcccgc tttcgccgca gcgtagttgg cctgaccgcc atttcccatg gtaccaacca
    49141 cagaaccgat agtgataata cgaccatgac gctttttcat catagcgcgc attaccgctt
    49201 ttgacagacg gaaaacagat gaaaggttag tttcgataat atcgttccac tcttcatctt
    49261 tcattcgcat taacaggtta tcacgagtga taccggcatt attgaccagg atatccactt
    49321 caccaaattc tgcgcgaatt ttttccagaa cagattcgat agatgccggg tcggtcacat
    49381 tcaacatcag acctttgccg ttggcaccta aataatcact gatcgcctga gcgccatttt
    49441 cactggtcgc agtgccaata actttcgcgc cacgggctgc gagcgtttca gcaattgcgc
    49501 ggccaattcc gcggcttgca ccggttacca gtgcgatttt tccttcaaaa ttcatgattt
    49561 tcctctttta aagctcgagc gccgctgcca tcgctgaagg ttcgttcagc gccgaggcgg
    49621 tcagggtgtc gacaatgcgt ttcgtcaggc cagtaagcac cttgcccggg ccgacttcat
    49681 agagatgttc tacgccttgc gctgccatgt actcaacaga cttcgtccac tgaaccgggt
    49741 tatacaactg acgtaccagt gcgtcacgga tggcatcacc attggtttcg catttcacat
    49801 caacgttatt cacaacagaa actgttggtg cgttaaaggt gattttcgct aattctactg
    49861 ccagtttgtc ggctgctggt ttcatcagcg cacagtgaga cggtacgctc actggtaacg
    49921 gcagcgcgcg ttttgcgcca gcagctttac aggcagcgcc agcacgctca accgcttctt
    49981 tatgaccggc aataaccacc tgtcccggag agttaaagtt taccggagaa acgacctgac
    50041 cttctgcagc ttcttcacac gctttcgcaa tagacgcatc atccagaccg atgattgccg
    50101 ccatagcgcc cgtgccttcc ggtacggctt cttgcatgaa cttaccgcgc atctcaacca
    50161 aacgcaccgc atcagcgaaa tcaatcacgc cagcgcaaac cagcgcggag tattccccca
    50221 ggctgtgacc ggccatcatt gccggtgctt taccgccctg ctgctgccat acgcgataca
    50281 gcgcaacaga tgcagtcaac agcgctggct gggtttgcca ggttttattc agttcttcag
    50341 ctggcccctg ctgggtcagc gcccacaggt cgtagcccag cgccgcagaa gcttcagcaa
    50401 acgtttcttc gacaattgga tagctcgccg ccatatcagc cagcattcca acggtttgag
    50461 aaccctgtcc agggaacaca aatgcaaatt gcgtcatgtt ttaatcctta tcctagaaac
    50521 gaaccagcgc ggagccccaa gtgaatccac cgccaaaggc ttcaagcaga accaactgcc
    50581 ccggcttaat gcgcccgtcg cgtacagctt catccagcgc gcacgggaca gaggccgcag
    50641 aggtattacc gtggcgatcc agcgtcacca cgacattgtc catcgacata ccgagttttt
    50701 tcgccgttgc actgataata cgcaggttag cctgatgcgg aaccagccag tccagttgag
    50761 aacggtcaag attattcgcc gccagcgtct catcaacgat gtgcgccagt tccgttaccg
    50821 caaccttgaa gacttcgttg cccgccatcg tcagatgaat tgaattctct ggattcacgc
    50881 ggtcggcgtt tggcagcgtc agcaattcac cataactacc gtcggcatgc agatgggtgg
    50941 aaatgattcc cggctcttca gaggcagcca gcaccgcagc gcccgcgcca tcgccaaaaa
    51001 taataatagt cccacgatct gttggatcgc aggtgcgcgc cagtacatcg gaaccgacga
    51061 ccagagcata cttcaccgcc ccagatttca cgtattgatc ggctacgctt aatgcatagg
    51121 tgaaacctgc gcaggctgct gcaacgtcaa atgccgggca acctttaatg cccaacatgc
    51181 tttgaatctg acaagctgcg ctcgggaaag cgtgcgtagc agaagtcgtt gccacaacga
    51241 tcaggccaat ctggtctttc tcaatgcccg ccatctcaat tgcgcgtgtc gccgcttcaa
    51301 agcccatggt tgaaacggtt tcgtttggcg cggcaatgtg gcgttcgcgg ataccggtac
    51361 gagtgacaat ccactcgtca gaggtgtcca ccattttttc caaatcggcg tttgtccgca
    51421 cttgttcggg cagatagctg ccagtaccaa taatcttcgt atacatgtac gctcagtcac
    51481 ttttcggtta tataccgtca cttgcaaact gcgagttcgc tggcagcgtc ctgctaccgc
    51541 agagttccgc ttttgccacc gtccagcagc tcaaaaccag ctgggtatac agattccagg
    51601 cgagcggcaa ttcgctgagg aacttgtcgc tgcaccgcct gcactgcctg ttcaatcgcg
    51661 accgcaaaag ctcgctgatt ggctgcacca tgacttttta tcaccgtgcc gcgcaatcct
    51721 aacagacagg cgccgttata ctggtcgggg ttgaggtgac tgaatcgcct cgtcaggctc
    51781 ttttgtagcc aacgctttaa taacagtagc caccacgacc gttttttccc ttcaccctga
    51841 gatttcagca gagaaaggaa catcctgaca acaccttcca tcgtctttaa cgtgacattt
    51901 cctgtaaagc cgtcacaaac cagcacatct gtcttgccag ttaacaactc attggcttca
    51961 agatagccga tataattgat agaagggatt gttttaagca ccgctgaggc atcccgaata
    52021 ctgtcaagac cctttacttc ttcttcacca atattgagca acgccacgcg aggattggga
    52081 atttccacca cctcttcagc cagaactgag cccataatgg caaattgcac caacattgtg
    52141 ctgtcacaat cgacgttggc ccctaaatcg aggaccaccg ttttgccctt ttgctgatgt
    52201 ggtaataccg tcaccagcgc cggacgctca atcccctcca ggggcttgag taataatttt
    52261 gccagcccca tcagtgcccc ggtattaccg gcactgacac aggcttgcgc tcgaccttct
    52321 ttcaccagct ccagggccat acgcattgaa ctcccacgac tggcgcggat agcttgcgaa
    52381 ggccgggcat cactggcgat aactgactgc gcaggaataa tctgcagacg cgaacgttgt
    52441 tcaaagtcag ctttagcaag taatggcgtg atggcgtcgg gattgccgac taaaagaaga
    52501 gtgagttgcg aattagaatt cagtgcctgc aatgctgcag gcactgtcac ggaagggcca
    52561 aaatcccctc ccatgacatc taacgccagg gttagacgtg tcaaggtatc gtcgccgccc
    52621 ggtttggtct ttccccagtt gcctggggaa aatcctcact aagcttcatc acgcagacgc
    52681 gtgattactt agcgatgacc ttgcggccgc ggtagtaacc gtcggcagtg atgtggtgac
    52741 gcaggtgttt ttcaccagaa gttttgtcta cagacaggct ggtgactgcg gtcagcgcgt
    52801 catgggaacg acgcatgcca cgtttggaac gggttggttt attctgttgt acggccatgg
    52861 accttactcc tcaattacgg tttatccccg atccaacggg gagcaccaat tacttacgct
    52921 ttaagctggc taatacggca aatgggtttg gcttttgcac ttcttcaggc agttcaccaa
    52981 agaccatgtc cgcttcggac acttcacagt gttcagaatc atgcaccgga actaccggca
    53041 aggcgaggat gatttcatct tcaaccattg caagcagatc gatttcaccg aattcgttaa
    53101 cctcaatcgg ttcatacgct tccggcagtg cttcagcctg ttcgtctgaa cgcacaggac
    53161 taaaacaata cgttgtgtag acctgatgag taaacggctt cccgcaacgc tgacactcga
    53221 gcgttaccgt caccttcgca tcgccgttta acactgcgag acgttggtta tcgatagcga
    53281 acgacatgga gcattccaca tcactgtcca cactgactac ggattcggcg acgcgctcaa
    53341 cctgatcagg ggtatagata ccctggtaat caaggcgttt ttgagccgta cgaaccggat
    53401 cgagagtcag ggggaatttt accttttgca tagggcgcgc atattaactt tgtaacgtca
    53461 tagagtcaaa gaaaaaggca gcctgtggtt gccttttgcc aataattcgc acacattgcg
    53521 ggttactgct ttattttcgt tcagtgctga taacgaacat caacctgcaa aacgaagcgc
    53581 atagtttaaa atggctatca tcaatacgct atatctgatg gaaaaaaata tgcctaaact
    53641 tattttagcc tccacctcgc catggcgtcg cgctcttctg gaaaaactgc aaatctcttt
    53701 tgaatgtgca gcacctgagg tcgacgaaac cccacgcagc gacgaatcac cgcgacagtt
    53761 ggtgcttcga ctggcacaag aaaaagcgca atctctggcg tcacgttatc cggatcattt
    53821 aattattggt tcagaccagg tatgtgttct tgatggcgaa atcaccggta aaccgttaac
    53881 ggaagaaaat gcccgtctgc aattacgcaa agccagcggc aatatcgtca ccttctatac
    53941 tggactggcg ttgtttaatt cggcgaatgg gcatctacaa acagaagtgg agccttttga
    54001 cgtccatttc cgtcacctaa gcgaggcgga gattgataat tacgtgcgta aggagcatcc
    54061 cctgcactgc gcgggtagct ttaagagtga aggatttggc attacgctgt ttgagcgttt
    54121 agagggacgt gaccctaaca cgctggttgg tttaccgcta atcgcgctgt gtcagatgtt
    54181 acgtcgggaa gggaaaaacc cgctgatggg atagttgttt gcgccggatg cggttacgtc
    54241 gcatccggca atcggtgcag gcggcctgat gcggcgtaaa cgccttatca ggccaaccgc
    54301 ccattatcag gcttatatgc ttgtattagc gcgcgttacg cagtttttgc aaacaacgct
    54361 tcaaaccttc atccatcggc gcttcgatac gcatcacctc accggtcccc ggatgggtaa
    54421 acttcaacgc tgcggcgtgc aggaacagac gatttaatcc cgtgcctgct tcagtgagct
    54481 gtctgtcaaa ttcacggtca ccgtagcgat cgtcaaaggc aatcggatga cccgcatact
    54541 gtgtatgcac acggatctga tgagtacgtc cggttaccgg gctacaacgc accagggtgg
    54601 caaatgcata gcgttcttcc actttaaagc gtgtttccga cggtttgcct tcctgactga
    54661 cacgcacgat acgttcgccg ctttgcagaa tatttttcaa taacggcgct tgaacgctct
    54721 tcacatgcga ctgccactga ccgcgcacca gcgccaggta atctttttgc attccttttt
    54781 cacgtaattg ctcatgcaga gaacgcaacg ccgagcgttt tttcgctacc agcaacacac
    54841 ctgaagtgtc ccggtcgaga cgatgaacca gttcaaggaa ccgcgcttcc gggcgcaacg
    54901 cccgcaaacc ttcgataacg ccgaagctta aaccactgcc gccatgtacc gccgtaccgg
    54961 aaggtttatt cagcaccagg atatgatcat cttcatataa gatgacgtcc gccagcgccg
    55021 ccaccttttg cagatgtggc gaaaccgcct cttcttcccg ctcagcaacg cgaaccggtg
    55081 ggatacgcac ctcatcaccc gcttcgagtt tatattcagg cttaatacgt tttttgttca
    55141 cccgcacttc acctttacgc aaaatacggt aaatcatact ttttggtacg cctttcaatt
    55201 gggtacgcaa aaagttatcg atacgttgcc ccgcttcgtc agcggtgata gcaacaattt
    55261 ttacggatgg agtctctgtt ttcatggtgg gcgattctaa ttagccaaca ggattcgcgc
    55321 cactcatttt tctatgctta tatttagttt tgcaccttat tacttcacta cgtgatcact
    55381 ttattgatgg ttattaaacc aatcaccagc aagaagtgaa aaaactgtga gtaagcgggt
    55441 gataaatggt aaaagtcatc ttgctataac aaggcttgca gtggaataat gaggccgttt
    55501 ccgtgtccat ccttgttaaa acaagaaatt ttacggaata acccattttg cccgaccgat
    55561 catccacgca gcaatggcgt aagacgtatt gatctttcag gcagttagcg ggctgcgggt
    55621 tgcagtcctt accggtagat ggaaatattt ctggagagta atacccagtc tgtttctttg
    55681 ataattgcgc tgtttttccg catgaaaaac gggcaaccga cactctgcgc ctctttgagc
    55741 tgacgataac cgtgaggttg gcgacgcgac tagacacgag gccatcggtt cacacccgga
    55801 aaggcgttac tttgcccgca gcttagtcgt caatgtaaga ataatgagta agttacgatg
    55861 aaaagaatgt taatcaacgc aactcagcag gaagagttgc gcgttgccct tgtagatggg
    55921 cagcgtctgt atgacctgga tatcgaaagt ccagggcacg agcagaaaaa ggcaaacatc
    55981 tacaaaggta aaatcacccg cattgaaccg agtctggaag ctgcttttgt tgattacggc
    56041 gctgaacgtc acggtttcct cccactaaaa gaaattgccc gcgaatattt ccctgctaac
    56101 tacagtgctc atggtcgtcc caacattaaa gatgtgttgc gtgaaggtca ggaagtcatt
    56161 gttcagatcg ataaagaaga gcgcggcaac aaaggcgcgg cattaaccac ctttatcagt
    56221 ctggcgggta gctacctggt tctgatgcca aataacccgc gcgcaggtgg catttctcgc
    56281 cgtatcgaag gcgatgaccg taccgaatta aaagaagcac tggcaagcct tgaactgccg
    56341 gaaggcatgg ggcttatcgt gcgcaccgct ggcgtaggca aatctgctga ggcgctgcaa
    56401 tgggatttaa gcttccgcct gaaacactgg gaagccatca aaaaagccgc tgaaagccgc
    56461 ccggctccgt tcctgattca tcaggagagc aacgtaatcg ttcgcgcctt ccgcgattat
    56521 ttacgtcagg atatcggcga aatccttatc gataacccga aagtcctcga actggcacgt
    56581 cagcatatcg ctgcattagg tcgcccggat ttcagcagca aaatcaaact gtacaccggc
    56641 gagatcccgc tgttcagcca ctaccagatc gagtcgcaga tcgagtccgc cttccagcgt
    56701 gaagtccgtc tgccatccgg tggttccatt gttatcgaca gcaccgaagc gttaacagcc
    56761 atcgacatca actccgcacg cgcgacccgc ggcggcgata tcgaagaaac tgcgtttaac
    56821 accaacctcg aagccgccga tgagattgct cgtcagctgc gcctgcgtga cctcggcggc
    56881 ctgattgtta tcgacttcat cgacatgacg ccagtacgcc accagcgtgc ggtagaaaac
    56941 cgtctgcgtg aagcggtgcg tcaggaccgt gcgcgtattc aaatcagcca tatttctcgc
    57001 tttggcctgc tggaaatgtc ccgccagcgc ctgagcccat cactgggtga atccagccat
    57061 cacgtctgcc cgcgctgctc cggtaccggt accgtgcgtg acaacgaatc gctgtcgctc
    57121 tctattctgc gtttgatcga agaagaagcg ctgaaagaga acacccagga agttcacgcc
    57181 attgttcctg tgccaatcgc ttcttatctg ctgaatgaaa aacgttctgc ggtgaatgcg
    57241 attgaaacgc gtcaggacgg tgttcgctgc gtgattgtgc caaacgatca gatggaaacc
    57301 ccgcactacc acgtgttgcg cgtgcgcaaa ggggaagaaa cgccgacctt aagctacatg
    57361 ctgccgaagc tgcatgaaga agcgatggcg ctgccgtctg aagaagagtt cgctgaacgt
    57421 aagcgtcctg aacaacctgc actggcaacc tttgccatgc cggatgtgcc gccagcgcca
    57481 accccagctg aacctgccgc gactgtcgta gccccagcac cgaaagctgc aacggcaaca
    57541 ccagcagctc ctgcacaacc tgggctgtta agccgcttct tcggcgcact gaaagcgctg
    57601 ttcagcggtg gtgaagaagc caaaccgacc gagcaaccaa caccgaaagc agaagcgaaa
    57661 ccggaacgtc aacaggatcg tcgcaaacct cgtcagagca accgccgtga ccgtaatgag
    57721 cgccgcgaca cccgtagtga acgtactgaa ggcaacgata atcgcgaaga aaaccgtcgt
    57781 aatcgtcgcc aggcacagca gcaaactgcc gagacgcgtg agagccgtca gcaggctgag
    57841 gtaacggaaa aagcacgtac caccgacgag cagcaagcgc cgcgtcgtga acgtagccgc
    57901 cgccgtaacg atgataaacg tcaggcgcaa caagaagcga aggcgctgaa tgttgaagag
    57961 caatctgttc aggaaaccga acaggaagaa cgtgtacgtc cggttcagcc gcgtcgtaaa
    58021 cagcgtcagc tcaatcagaa agtgcgttac gagcaaagcg tagccgaaga agcggtagtc
    58081 gcaccggtgg ttgaagaaac tgccgctgcc gaaccaattg ttcaggaagc tccagctcca
    58141 cgcacagaac tggtgaaagt cccgctgcca gtcgtagcgc aaactgcacc agaacagcaa
    58201 gaagagaaca atgctgataa ccgtgacaac ggtggcatgc cgcgtcgttc tcgccgctcg
    58261 cctcgtcacc tgcgcgtgag tggtcagcgt cgtcgtcgct atcgtgacga gcgttatcca
    58321 acccagtcgc caatgccgtt gaccgtagcg tgcgcgtctc cggaactggc ctctggcaaa
    58381 gtctggatcc gctatccaat tgtacgtccg caagatgtac aagttgaaga gcagcgcgaa
    58441 caggaagaag tacaagtgca gccgatggtg actgaggtcc ctgtcgccgc cgctgtcgaa
    58501 cctgttgtta gcgcgccagt tgttgaagaa atggctgaag tcgtagaagc ccccgtaccg
    58561 gttgccgaac cgcaaccgga agtggttgaa acgacgcatc ctgaagtgat tgccgccgcg
    58621 gtaactgaac agccgcaggt gattaccgag tctgatgttg ccgtagccca ggaagtcgca
    58681 gaacacgcag aaccggtggt tgaaccgcag gaagagacgg cagatattga agaagttgcc
    58741 gaaactgctg aggttgtggt tgctgaacct gaagttgttg ctcaacctgc cgcgccagtc
    58801 gtcgctgaag tcgcagcaga agttgaaacg gtagccgcgg ttgaaccaga gatcaccgtt
    58861 gagcataacc acgctaccgc gccaatgacg cgcgctccgg caccggaata tgttccggag
    58921 gcaccgcgtc acagtgactg gcagcgccct acttttgcct tcgaaggtaa aggtgccgca
    58981 ggtggtcata cggcaacgca tcatgcctct gccgctcctg cgcgtccgca acctgttgag
    59041 taataattag ctcaaagtaa tcaagccctg gtaactgcca gggctttttt atttcatgtt
    59101 tgaatcaaca tattaaaatt catcaaatgg catccttgct aaccaacaat gcaaaatagg
    59161 caaaaaaaat gcccgccccg gcgatcccag agcgggcaga caaaacatac ccagtttcat
    59221 gatatgtttt aaagcgagct tatttgttga gctggaagag cgacaatccc tgcatatcgg
    59281 taaatgcttt atacgatgcc tgcaatgccg tttgctgcat gatgtaagat gaaatagttg
    59341 cattccagtc cacatcaacc agatcgctca tctgctgcgt ttgccctaaa gcgcgatcgc
    59401 tacctaagga atccagcgac tccagtttgt tcagctgcgt gcctaattcc gtgcgaacgg
    59461 taaggacatt attcagcgag tttttcagtc cacggttggt tttatccaat gccgcagcgg
    59521 cgatttcttt atccgcttcg ctatccgcga ccggcgtttt cagggctgcg atggcgctat
    59581 ccagcatggc aaaaagattg gtttcagaag cgctaccgtc tggttccgct accgcgttac
    59641 tggtaatact gtcgaaaatt ttgtcgcccg tatgtcctat caccatcgaa cgcgaagcat
    59701 cgacctgttg cttaatactt tctgttccgc cgacataatc accgttagcc tcactaaacg
    59761 gcgcagtctc tgttttataa ccggcaaaaa tgtagcgccc attaccgtca gtggtgttcg
    59821 ccagattcag caactggtca cgaagcccct gaatatccgt agccagcgag gcccggtcat
    59881 cgtcactcaa ggtgccattg ctggcgtaga caattttttc ctgagcattc tggatagcag
    59941 tggtgacctg gctaagtaca ctctcttcca gtgacacttt ttgagtggcg aaagtacgtg
    60001 ccagcgtgta ctggctgttt tgcgcctgtg cctgggagag aacaacagct tgtgatgcag
    60061 caatgggatc gtctgaaggg ttaacgactc gcttacccgt caacatctgc tcgccgtact
    60121 tcatccattc tgcctgagaa ttggtgatgc cgcgcatgtt ttgctggtac atcatctggg
    60181 tactgaaacg cattttatct ccccttagcg aatgttaatc agcgcatcaa aaatcgcgtt
    60241 tgccgtctgc agaacctgcg cattcgccag gtaatactgc tgaaaacgtt gcagatttcc
    60301 gtactcctca tcgagattga caccggaaat cgactgctgc tgattggaaa gctgcgtcac
    60361 cacattacct tgcgtggtgc tactggtttt caacgtcgcg gttttattac cgatatcact
    60421 cactaacgag gcataagcgt cgttaaagga tttcgcaccg cccaccgttt tactgttgct
    60481 ttgcagatcc agcagggcct gaccgttgcg gttgtcgcta tcacccgcat cttcttcgct
    60541 cgccatcgcg attttcgctt cgtcggtgat taatacatcc atgttgacga tggcgtcact
    60601 tactggtttc agcgtgaagc tgtcgttaac ggcaggcgtt cctgtaaacg tcaactccag
    60661 accatcaaat gccactttac cgttggcatc tggtgtcgcc gtaaaagtgg tattgctggc
    60721 aaggcgggtg acctgccact gattattatc gaacgagatt ttgtaatctg tcgccagtac
    60781 cacggaggca tcagttaccg tggcaccgat cgcaacgtca cctttgtttt tcgtgttttg
    60841 ctgaaccgcg ggcttaccga tagtaaagaa atcttcacca gcatcgccgt tagcatcaaa
    60901 tccggctttg tgttggctgt tgaaagcctc ggcaaatgcc agcgccagtt gtccaagcgt
    60961 attacgcgtc tggtccagat cctgagaacg gaatgtcaga atgccgccca gcgacccggt
    61021 attcagtaat ttctccggga tctcaatatt gcctgccgtc ccatcaacgt aagcgacagt
    61081 cgtacgagaa gggtcggcgc tggaaggaac tgccgccagt tgccgcgccg tacttccctg
    61141 aaccagtgag taaccattgg catcgtgatg ttataagtac cgccatcctg aacgctgact
    61201 tctacgccaa caatctggtt taattcgctc accagttgat cgcgttgatc cagcagattg
    61261 ttaggtgacg cccctgcccc cacgcctgtc aggcgcgaga tttgatcgtt caggctggca
    61321 atttgtttag cgtaattgtt gatctgatca acgctggcac ctatcgcgat attgacctgt
    61381 ttgtcctggt cgcgcagata ttgatcggtg gttttaaact gattcaccaa cccttctgat
    61441 ttcccaatca gcgcctggcg cgccgccggg tcttccgcgt tactcaccag cgtttgcagg
    61501 ctggtgaaga aatcctgcat ctgtgttgcc agcgaagagg tactggtgga gagcatattg
    61561 tcgattttcg acatctgctc atagcgggcg gtcaggccgc tactttgcgt ctgcacagca
    61621 cgtaactggt tggtaataaa cgtatcatac tcacgctgca caccagaaac gtagacgcca
    61681 ttgccaaccc agccgccagc gcccaacgtg ctattggcct gcgccataat agtggtttgg
    61741 cgggtatatc cggcaacgtt atagctggag atattattac ttgccgtatt taacgccgcc
    61801 tgggccgcgt tcagtccgct catggcgtta ttgatcaagc tggacatgga ggttccttat
    61861 aagccttcaa ttacagagta ttatcggcag cgacccgccg gacttgagtt attcagaaca
    61921 gattatcaat gttcatgctg taggttttgc tcacctggtc gcttatcgat ttcatctgct
    61981 ggatcatgtt ggtgagttta cgggcatagt gaggatcggt agcatagccc gcgtcctgta
    62041 ggacctgcgc cccctgttcc gcactcgcgg cggtcgtcac ggcggcgtag cgcgggttac
    62101 gtgttaacag cccaacgtaa tccgacaatg cttccagata cgagctgtag acccgaaact
    62161 tcgcttttac tttcttcgct tcgccgtttt catattcagt cgtggtgatt tcagtgactt
    62221 gccctttcca gttgccagag gctttgacac caaacaggtt atagcttggc tcgccgtttt
    62281 cgcggcggat ttgccgttgc ccccaaccag attcaagcgc agcctgagcg aggatcaaat
    62341 gatgtggcac accgctttgc tggcttgcca gttgggcggg cagcgagagt tgcgcgagga
    62401 atgctctact gtcacccggc agcgaatcat cgtagttacg tggtacggcc ttttgcacca
    62461 gctgcgaaag cgtctggttt tgataacgca ccacggtttc gagcgggaat ttcatcggtg
    62521 ctgctggcgt ggactcctct ggcaatggtt gttctggcgt catctgttta accatcatct
    62581 ctgcaagccc cagacctttg cccgccgtca tctgttgggc aatttgctgg tcatacatac
    62641 tggtatacag acgagtgtgc tcgctgctga acaggccatc ttttggtaaa gcgtcgcgca
    62701 tgcttttcaa catcatctgc acgaacatcc cttccacctg acgggccacc ggacggatat
    62761 ttgccgccgg atcttcgctc gctttcgcct ttagttcgtt gagtgattgc gcatcccagg
    62821 ccgcacttgc cagtagtttg ctgtcgctga tcatcagatg atttccagtt ttgcccgcag
    62881 acatcccgca ctttgcattg attgcaaaat agacatcaga tccatcggcg tagcgcccag
    62941 cgcattgagc gcacgcacca cgttattgag gctggcgctg gaacgtacgc tttgcagcga
    63001 accgccgctc tggcgtaaat cgatctgcgt ttgtggagta acaacagtct gtccaccacc
    63061 aaacggtgta tctggctggc tgacattggc ctgacgatta actgttactg agagatttcc
    63121 ctgcgctacc gcgcagctgt cgagggtcac ttctcgattc atcaccaccg aaccggtgcg
    63181 cgagttaatc actactttag cgtcctgcgg ggtgacatta acctgcatat tctggatatc
    63241 ggcaaggaaa cggacctggg aactgttgcc acttggtacg cgcacctgaa tagtccgcgc
    63301 atctaacgcg gtggcgctgc catatccacg cacgcggttg atggtgtcag cgatttgctg
    63361 cgccatgctg aaatcttcgt cgttaagttg caaattaagg gtattcccga cgccaaactg
    63421 gctgggcaat tcacgttcaa taaccgcacc attggtgatc cgtccaccgt tcagttggtt
    63481 aacctgaaca ctgctaccgc ccgcggaggc tcctgcgccg ccaaccagaa tattgccctg
    63541 cgccagcgca tacacctgac tgtcaacgcc cttaagcggt gtcatcaaca acgtacctcc
    63601 acgcaagctt ttggcattac ccatggaaga aaccaccaca tcgatggttt gcccctgacg
    63661 tccaaacgga ggaagtgacg ctgtcaccat taccacagcg acgtttttta gctgcatatt
    63721 ggtgcccgtc ggaacggtaa ttcccagctg tgagagcatg ttattaagcg tttgtgtggt
    63781 aaacggcgtc tgggttgtct ggtcaccagt gccatccagc cccactacca ggccatagcc
    63841 aatcagtgag ttttgcctta ccccctgaac actggtgaga tcgcgaatac gctcagcctg
    63901 agccgccgtc gtaaccagta gaagaattaa tgcagagaga aatttaatca ccacagcctc
    63961 acttacattg gcgacaggtt aaggaagaaa cgctgcaacc agcccatatt ttgcgcttcg
    64021 ttaatgtagc cattgcctac gtattcaata cgcgcatccg ccacctgagt agacggtacg
    64081 gtattgctgc cgctgatagt gcgtggatta accacgcccg agaagcgaat aaattcggta
    64141 ccctgattaa tggcaatctg tttttcaccc accacatgca ggttgccgtt gaccagtacc
    64201 tggtcaaccg tcaccgtcaa cgtgccgcta aaggtattgc tggcattggc cccgcccttt
    64261 ccgttgaacg tgttaccacc ggaggcttcg acatcggcac gagcgttacc aaacagcccc
    64321 tgcaaatagc gcggcacagt atcaaagcca aaattagttt taccgtcacg gctggcattc
    64381 gcagaggagc ttttgctggc gctgacgttc tcctgcaaca cgatggtcag cgtatcgcca
    64441 atattgcgtg gtcgacgatc ttcaaacagc ggttgatagc catagttaat cggctgagca
    64501 gactggaaaa tagaaccgtt ggcgacgggc gtcggaccgg gaaccggttg tgcactggtc
    64561 gccccctgca ccagcggcgt ggagggtatc caggcgcagc cggttagtga aagcaccaac
    64621 aagctggaaa tggcataagt atgcgcagcg tttttttgca tggctatcat cttcaaaaat
    64681 cagtaaaccg gtgaacctgc caccggttat gccttagagt tgcgtcagtt tttgcagcat
    64741 ctgatcggtg gtggacaccg ctttactgtt gatttcgtaa gcgcgttgca cctgaatcat
    64801 attgaccagt tcttccgcca cgttgacgtt agacgtttca acataccctt gatacagcag
    64861 tcccgcgccg ttcaggcccg gcgtgctttc gttcggtgca ccggaggatt gcgtttcggt
    64921 gtagaggttt tcgccaatgc tctccagtcc ggtatcattc ataaaggtgg tgagattgag
    64981 ctgcccaacc tgaaccggag ctgcctggcc ttgttgggtt acgctgacca cgccatcacg
    65041 accgatggtg atacttaacg cattcgccgg aatggtgatc gctggctgca cctgaaaacc
    65101 accagccgtc accagctgcc cgttctgatc cacctggaaa gagccgtcac gggtataggc
    65161 tgatgagcca tctggcaaca ttacctggaa aaagccctgc cctttaatcg cgacatcttt
    65221 gctgttattg gtctgcgaca gattgccctg gctgtgtaag cgttcagtgg cgaccgggcg
    65281 tacccccgtg ccgatttgta atccggaggg taaggtggtt tgttcggaag actgtgcccc
    65341 cggctggcga atggtttgat aaagcagatc ttcaaacacc gcgcgctgac gcttaaaacc
    65401 gttagtactg acgtttgcca ggttgttggc aatgacgtcc atattggttt gctgggcgtc
    65461 aaggcccgtt ttggcgatcc ataatgaact gatcatataa tgtcctgtag tggattgctg
    65521 acaagcagag cagaagcacg cgacagtgtg aatttcccct caccctaacc ctctccccaa
    65581 aggggcgagg gaatagtccg cgcgctttta ttgcaatgtc atcaatcgtt caattaactc
    65641 atcgacagca gttggttggc acggcctgcg ttatcatcga cgctgctgat caccttcatc
    65701 tgcatttcaa aacgccgcgc gctggcaatc atgtcgctca ttgccgcaac ggcattgacg
    65761 ttactgcctt ccagaacccc cgacatcaca cgcaaggttg gatctgcctg cagtaccggc
    65821 cctcgcgtgg cctgggtttc tgcgcttaaa cgaaaaatgc cgtcgtcacc gcgctgcact
    65881 tcgctgcccg tggctttcac cagtttcaga cgccctactg gcgcaactgt atttgccgga
    65941 tcgcccggat tgagcgccga gattgtgcca tcggcagcaa tcgtgatttc cgccccttcc
    66001 ggcacagcaa ttggccccgc ctcgcctatc accggatgcc cctgaattgt cagttgcccg
    66061 gtgggatcaa cctgaatgct gccattacgc gtataccctt cgctgccgtc agcggtctgc
    66121 acggccagcc atcctgctgc aacgcaacgt ccagcgggcg cgaggtgtaa tccatcttgc
    66181 cgggcgtcat gtctgcaccc ggtgttgacg ccgttaccag cgtgcgcgtg ggcagtgaaa
    66241 gcccttccac cggcactgcg cgcaaggcgt ttaactgcgc gcgaaaaccg ggcgtagagg
    66301 cattggccag attactggcg gttaccgcct gttgattcag cgtctggctg gctgctccca
    66361 tcgcagtata aattgcgtga tccattgagc tatcccgtca gcgattagcg taagttaacc
    66421 cgcgtgttga ggatctggtc ctgggttttg atggtctggg cgttagactg atagttacgc
    66481 tgggcaacga tcatattgac cagttcttta ctgagatcga cgttggacgc ttccagcgca
    66541 ccgttggtca gggtgccaaa gtttcccgtc ccggctgtcc ccaacagcgc cacgccagaa
    66601 gattgcgtcg cagaccagac gttgtcgcct tcggatgcca gaccttcgtt gttggcaaag
    66661 ttcgccagta caatctgccc cagcagttgg gtttgttcgt tggaatagtt gccgacaacc
    66721 gtaccgtcat cattgatttg ataactcacc agatcgcccg gtttgtagcc attctgggtg
    66781 gttgccacaa tgttgttagc gccggtattt tgctgcatgg agttgaggaa gctcagacta
    66841 aacgtggcgg gttctgcacc gttaattgcg ccggttgcga tattattcgc catcgcacca
    66901 tccactaatg tgccattagc attaaattcc agtgttgtcg ctgtcttcgc aatgctgttt
    66961 ggatcactgc tatcctgggt gtagacctgc cagttattat ccccggtctt cacaaaatag
    67021 acgctcatgt catgagcatt accctgactg tcgaaaacag tcaccgaacc ttttttgtta
    67081 tagctatcca cattgctggc gctgaatggc gtaacagtag gaagcggatc actggaattc
    67141 aggttgatct gcatcgacgc cgtggtggta gttttcgctg ccatcagggt attcgggatc
    67201 gaaatattgg tcggattcgc cccttgctga atagtcggcg gcgtaccggt tgccgggtaa
    67261 cccgtcagct gtaaaccttg catattcaac aggttacggt tttcatccag cttaaattgt
    67321 ccgttacggc tgtagaacac cgaaccgttg ctgtctacca gacggaaaaa accgttctgg
    67381 ctgatagcaa cgtccagacc tcgcccggtg ttggtggtcg tgccatcggt aaagtcctga
    67441 gtgataccgg caacttttac ccccagtccc actttcgaac cggcaaacat atcggcaaaa
    67501 gaggccgtgc ctgatttaaa gccgtaggtg gcggagttgg cgatattgtt gccaataaca
    67561 tcgaggttgg tggcggcagc gtttaatccg ctaaccgctt gagaaaaggc catgactgac
    67621 tcctgaaatg tgaaggctca aattatctgc cgtacttcgt cgagggtggt ggtgccgtaa
    67681 gtgccgagat ccagcgtatt accgctgttg ccgcggatca caccctgcac cagagcaaac
    67741 tgcagcggct gggcaaccag ttgtgtacca ccgttactgg cgctaatcgc tacattgtaa
    67801 gaaccgttcg gcgcagtgct gccatcagtc aacgtaccgt cccaggtgaa actgtgaact
    67861 ccggcggtca gttcaccaat atcaatggtg cgcacaaccg cgccattttt atcggtgatg
    67921 gtggccgtaa ctttgtctgc cgcctgttgc agctcaacac caaacggcgt ggtcgtggtc
    67981 acagcccctt cttcactgcc ggttcccgca agaacagttg tgccggggat catcacgccg
    68041 tgaccgatca ggttactggc ctgtaacgac tggctgttat caatctgtcc ggaaatagat
    68101 ccgagcgtgg tattgagttt ttcaatcccg ctgaccgtgc tgatttgtgc caattgcgac
    68161 gtcagctcgt tgttttccat tggattggtc gggtcctggt ttttcagctg cgccaccagc
    68221 aaagtcagaa aactgctttg taaatctgcg gcgttgctgc ccgtgagcga actactgctg
    68281 gtggtactga cgccggtatt tgtcggatcg gtggtggtta ccgcaatgga catagctttc
    68341 tcctttattg accgagcgta agggttttca gcatcatgct tttcaccgtg ttgagcactt
    68401 caacattggc ctgatagctg cgtgacgccg acatggtgtt aaccatctcc ccgacgacat
    68461 caacgttcgg catttttacg tagccctttg catctgccag cggattacct ggttcataaa
    68521 ccagtttgtc cggggcctga ctttcttaac atcggcaacc tttacgccgc ctgtcgcagc
    68581 acctggtgca gcgttaacct ggaataccac ctgttttgcc cgatatggct gtccatcagg
    68641 accggtcacg ctatcagcat ttgccagatt actggccgcc acgttcaggc gctgggactg
    68701 ggcagttaac gccgacccgg cgatatcaaa aatattcagc agtgccatcc gttaatttcc
    68761 gctctgtaaa acgttcatca tgcctttgat ttgcccgctc aacgcgctaa ggctcatctg
    68821 gtattgcagg ctgttatcgg caaactgggt gacttcctga cgctgggcgc gcagattgag
    68881 cgcctcttgt tgaaaacgta aggcggcgtc gagcttatcg agcatatctc ctccgcaggt
    68941 atcaaaattc tgccatcagc ttaaatgcct ttacgcgcgc gttatcggcg gaataaacgc
    69001 aaaatgggtc gctatttatg ccgttgatgg tcattgcgga caggtacaac tcacgttgta
    69061 gaaatggctg ggggcgaaaa tgctgacaat aaaacgtagc gtggcgatca tcgcgatact
    69121 gttcagtccg ttaagtacgg cgagcaatct cacatcgcaa ttgcacaact tttttagcgc
    69181 ccaactcgcg ggggtaagtg atgaggttcg tgtttctatt cgtacagcgc ccaatctact
    69241 accgccatgc gagcagccat tgctttcgat gagcaataat tcccgcctgt ggggcaatgt
    69301 gaatgtgttg gcacgctgcg gtaacgacaa acgatattta caggttaatg tgcaggccac
    69361 aggaaattat gtggttgccg cgatgcctat tgtgcgggga ggaaagctgg aagctggcaa
    69421 tgtcaaactg aaacgtggac gcctggatac cctgccaccg cgtacggtgc tggatatcaa
    69481 tcaacttgtt gatgccgtta gcctgcgcga tctatcaccc gatcaaccta tccagttaac
    69541 ccagtttcgc caggcatggc gggtaaaagc gggacaacgc gtcaatgtga tcgccagcgg
    69601 tgatgggttt agcgccaacg cagaaggtca ggcgctgaac aatgcagccg tcgcacagaa
    69661 tgcgcgggtg cgcatggtat cgggacaggt agtcagcggc gttgttgatg cagatgggaa
    69721 tattcttata aacctgtaag ctgttaaaga ttacccgtcc cttgccgata aataagcaac
    69781 acatgataaa agcgccctca atgaggaata aaccatgagt attgatcgca cttcgcctct
    69841 gaagcctgta agcaccgttc aaccgcgcga aaccactgac gcgccggtaa cgaacagccg
    69901 ggcggcaaaa acaaccgcct ccaccagcac cagtgtgacg ttaagcgacg cgcaagcaaa
    69961 actgatgcaa cccggcagca gtgatatcaa tcttgaacgt gtcgaagcgt taaaactggc
    70021 gattcgtaac ggtgaactaa aaatggacac cggcaaaatt gccgatgcgc tgatcaacga
    70081 agcgcagcaa gacttgcaga gtaactgatc gtatgacacg tcttgcagag atcctcgacc
    70141 agatgtccgc tgtgcttaac gatctcaaaa cggtaatgga tcatgagcag caacatctct
    70201 ctatggggca gatcaacggc agccagttgc aatggattac agaacaaaaa agctcactgc
    70261 tggcgacgct ggattacctc gaacagttac gcaagaaaga acccaataca gcaaatagcg
    70321 ttgatattag tcaacgctgg caggaaatta ctgtgaaaac gcagcaacta cgccaaatga
    70381 atcaacataa cggctggtta ctggaaggac agattgagcg caatcaacag gcgctggaaa
    70441 tgttgaaacc gcatcaggaa ccgacgctat atggggcgaa cggtcagacc tcaacacccc
    70501 atcgcggcag taaaaagatt tcgatctgaa tattatctcc ggcctgcact gcaggccgga
    70561 atgcattgtt acaccgtccg gcgggcaaat tctttaactt tgaagcccag taccgccagt
    70621 gcagcgaagt acgcggcaat ccccgccagc acgaccgcca ttaaacgcag taagcgccag
    70681 ggcatggtac ccaatgacca ctccggcatg atatgtaaca tacctaaaag cacgccagac
    70741 atcaccagta ccgccaccac cagacgcaac agaaacgcca tccagccggg ttgcggagta
    70801 aagattttct gcttacgcaa ctgccagtaa agcagcgaag cattcagaca cgccgccaga
    70861 ccaatagaaa gtgacagccc ggcatgtttc aacggaccaa taaacgccag gttcatcaat
    70921 tgcgttaaaa tcagcgtaac gatggcaatt ttcactggcg ttttaatgtc ttggcgggaa
    70981 taaaagccag gagccaacac tttcactaca atcaggccaa tcaaacccac cgagtaggca
    71041 attaacgccc gctgggtcat cagcgcatca aacgcggtaa atttaccgta ctggaacagc
    71101 gaaacggtta acggacctga aagaatgccc aacgcaaccg cactcggcag tgccaacagg
    71161 aaacaaagac gcaaccccca gtccatcaaa cggttgtatt catcatgatt gccactggca
    71221 aaacttttcg acagcgacgg cagcaaaatg gtgccaagcg ccacgcccag cacaccggag
    71281 ggaaactcca ttaagcggtc ggcgtaatac atccaagaca ccgaaccgga agcaagaaac
    71341 gaggcaaaaa tggtgttgat gattaaggag atctggctca cagagacgcc aaggatcgcc
    71401 ggtcccatct gtttcaccac gcgcattgct ccagcatcgt ggaagttaat gcgcggcagg
    71461 accagcatgc cgatcttctt caggtgcggt agctgataca ccagctgcag gatgccgccg
    71521 accgtaacag cccacgccag cgccagcacc ggtgggttaa agtacggtgc ggcaaacagc
    71581 gcgaaaccaa tcatgctgat gttaagcagt gttggcgcaa acgccggaat cgagaagcgg
    71641 ttccacgtat tcagaatcgc tcccaccagc gacgccaggg agatcagcaa gatataggga
    71701 aaggtaatct ttagtagctg gctggtcagg gcaaatttgt cagctgtgtc agcgaagcct
    71761 ggcgcggtca ccatgatcac ccacggtgcg gcgagcatgc cagcgaccgt cacaaccgcc
    71821 agcgcaagtg tcagcaggcc agaaacataa gagacaaaga cccgcgtggc gtcttcaccc
    71881 tgcttacttt tatattccgc cagaatcggt acaaatgcct gggaaaatgc cccttcggca
    71941 aagatacggc gtaacaagtt aggaagttta aaagcgacga aaaaggcgtc ggttgccatc
    72001 cctgcgccaa agattctggc gacaattgcg tctcgtgcga agccaagcac acgcgaaaac
    72061 atggtcatcg agctgacggc ggccagcgat tttaataaat tcatcggtgt tctaatccgg
    72121 acccacgggc aaaacgcctg caaggcaggc gctgaaagag gcgaatagtc tacctagatt
    72181 atggtgaatt gctaccgcca gatgttacag ggtttattca ctcatcgcat cgcgccagat
    72241 cttgtcaacg atacgttgcg ccagcactgc ctattcgccg gcggtttgcg gaactgtctg
    72301 attttgcacg cattcaatga agtgccgcgc acagccgaca aatccacgtt gctcaagagt
    72361 gctctgccaa ccaggaatcg gtttattcac tacaccctgc ccgcgctcct cacgccattc
    72421 gcgcatatcc gtaatgtcga tgagcgcacc gtcagtcacg gcctgcacgg tttcacgctg
    72481 acttccggca cggcgatgca tacaagtggt gatctgcaaa ggaccggccg aaaaatggtg
    72541 ctcggcaaac agcatttcgc cagcgtcgtt agtcagtagc gtaccgccat ccagagaggc
    72601 tttgccgccc gacaaccaca gcgcggtatc caccacatgc agataatcat ccagcaacgt
    72661 gaaataaaga tcgtgtggcc cgacgctatt gctacgatgt ttatccattc ttagcgaggc
    72721 tgcggtggcg agttgcgttt ttaactcacc gtagagtggt gcgaaacgac ggttaaaacc
    72781 gaccatcaac gtcagttttt tccgcgccgc cagttccacc agcctttcag catcgcgcag
    72841 attttctgcc agcggtttat cgacacagac atgtaccccc gcattgagta acgtactgac
    72901 cacgtcaaag tggctggcgg tgctggagtg cacaaaaacc gcatcacaac tggcggcaag
    72961 gctggataac gaatcggcat aaggaatgcg ccagctgtca caaattggca gggctttggc
    73021 gcgcgtaggc gaccaggctc cttgtaacgt ccagtcagac gctgccgcca gcaccggtaa
    73081 ccacgctttt tgcgcaatgc cgcctaatcc cactacgccg atacgtaatt ttttcacttt
    73141 aatctcccag atgggccagc aacgaatcaa gacgttgttt cagttctgcc acttcgattt
    73201 ccagggcttc gacgcgggtc tgtaaatcac cgtcaaccat gttcgacata gccgttaccg
    73261 ccggctgatc ttcaacctca ccactgaaaa gatgcatgta gcggctttcg cgtttacccg
    73321 gttcgcgggc cagacgcacc acaaaaggac catcttcgcg attcgccagt tgttccagcg
    73381 tcgactccac ttccgccata tcgctgaatt catacattcg cgcggcgcgg ctgcgcagtt
    73441 cacccagcgt ctgggcacca cgcaataaca acgtggtgat taacgccact tctgcggcgc
    73501 tcagtttcag atcgccaaat tctgaattac aaaaacgttg ctcatatttg gtgacccgat
    73561 taccaaaacc gctcactgtg cgtagataat gacgtttgac cagattatcc agctgttcct
    73621 gcacttcgga ttcactcaga ttcatgaccg gttcacggtt cgttttctga ttacaggccg
    73681 tgactacacc attgactgag agcgggtatt gctccggcgt ggtcacctgc ttttccagca
    73741 agcaaccaat tacgcgagct tccagggcag taagttgata tttcatgaga ttctccttag
    73801 cggccgggcg tccagtctgg ggtagttaat gccgtcagta cgtgatcgcg ccattgtcca
    73861 tcaatcaaca gatagtcttt cgcatagcct tctttttcaa aacccagtcg cgccagtaaa
    73921 tcaccgctgc gtttattgtg cggcatataa ttagccataa tgcgatgaat atgttgggtg
    73981 cgctgcatat aacgaatggc tgcggtcagg gcttcaaaca tgagtccttt gccctgccat
    74041 ttttgcccaa tcgaataacc gagatagcag gtatgaaaag agccgcgaac aacattggaa
    74101 aaattggcaa cgccaataat ctctttttcg tccgggtcga ataagccaaa gtagaaagct
    74161 gaaccttgtt tatgaaattc gttaatcatc cctagcctgg cctgccagcc tgatggataa
    74221 cagtggcttt cgtcgcgcac tggctcccag ggcttgagga aatggcgatt ctctgcgtaa
    74281 taatccgcca gacgccaggc atcacgatca tgcaccagac gcacgaccag tcggtctgtg
    74341 gttaagcgca cttttggcac gttactgcga tagccaaaca ttctatacct actccttccc
    74401 gtaacgtctt taatacgctg taagtattac tatacctgcg caaccagacc ctgtgaaaag
    74461 tccaaaatgc cattttctgc attattcaca tatttgataa aaactctcta tcaaagcgac
    74521 gttttgataa aaaaatattg tccccgaagt gatgggaaaa gtcttagcgg caatgcagaa
    74581 tagaaagtgc tcatctttta tttcccggga ggggaaatgt cccgcgtatc gcaggcgagg
    74641 aacctgggta aatatttcct gctcatcgat aatatgctgg tcgtgctggg gttctttgtt
    74701 gtcttcccgc tgatctctat ccgcttcgtt gatcaaatgg gctgggccgc cgtcatggtc
    74761 ggtattgctc tcggtctacg ccagtttatt cagcaaggtc tgggtatttt cggcggcgca
    74821 attgccgacc gctttggtgc caaaccgatg attgttaccg gtatgctgat gcgcgccgcc
    74881 ggattcgcca caatgggtat cgcccacgaa ccgtggctat tgtggttttc atgcctgctc
    74941 tcgggactcg gcggcacgtt gtttgatccg ccgcgttcgg cgctggtggt gaaactaatc
    75001 cgtccacaac aacgtggtcg ttttttctcg ctgttgatga tgcaggacag tgccagtgcg
    75061 gtcattggcg cattgttggg gagctggctg ttgcaatacg actttcgcct ggtctgcgcc
    75121 acaggggccg ttttgtttgt gctatgtgcg gcgttcaatg cgtggttgtt accggcatgg
    75181 aaactctcca ccgtacgcac gcccgttcgc gaaggcatga cccgcgtgat gcgtgacaag
    75241 cgttttgtca cctatgtcct gacgctggcg ggttactaca tgctggctgt acaagtgatg
    75301 ctgatgctgc caattatggt caacgacgtg gctggcgcgc cctctgccgt taaatggatg
    75361 tatgccattg aagcgtgtct gtcgttaacg ttgctctacc ctatcgcccg ctggagtgaa
    75421 aagcattttc gtctggaaca ccggttgatg gctgggctgt tgataatgtc attaagcatg
    75481 atgccggtgg gcatggtcag cggcctacaa caacttttca ccctgatttg tctgttttat
    75541 atcgggtcga tcattgccga gcctgcgcgt gaaaccttaa gtgcttcgct ggcggacgca
    75601 agagctcgcg gcagctatat ggggtttagc cgtctgggtc tggcgattgg cggcgctatt
    75661 ggttatatcg gtggtggctg gctgtttgac ctgggcaaat cgacgcacca gccagagctt
    75721 ccgtggatga tgctgggaat tattggcatc ttcactttcc ttgcgctggg ttggcagttt
    75781 agtcagaaac gcgccgcgcg tcgtttgctg gaacgcgacg cctgatttga tacacccccc
    75841 tgactcctcc atactgaaag tgaactgaca gccaaatgga ggagtcacgt gaagctatac
    75901 atttacgatc actgccctta ctgcctcaaa gcccgcatga ttttcggcct gaaaaatatc
    75961 cccgtcgaat tacatgttct gctcaacgac gacgcagaaa cacccacccg gatggtcggt
    76021 caaaaacagg ttcccattct gcaaaaagat gacagccgct atatgccaga aagcatggat
    76081 atcgttcact atgtcgataa actcgacggc aaaccgttac tgaccggcaa acgttcccct
    76141 gccattgaag agtggctgcg caaggtcaat ggctacgcca acaaactgct gttgccgcgt
    76201 tttgccaaat cggcatttga tgagttttct actcccgccg cgcgcaaata tttcgtcgac
    76261 aagaaagagg ccagcgcggg taattttgcc gacctgctgg cccactctga cggtctgatt
    76321 aagaatatca gcgatgattt gcgtgcgctg gacaaactga tcgtcaaacc gaacgccgtg
    76381 aatggcgaac tttcggaaga tgatattcag ctatttccgc tactgcgtaa tctgacgctg
    76441 gtagccggaa tcaactggcc aagccgcgtt gctgattacc gcgataatat ggcgaaacag
    76501 acacaaatca atttgttatc atcaatggcg atttaatcct gacccgccgc ggcagcctgt
    76561 attcgcgggt tgccgccgtt ttcaattttt tctgctggcg agtttatcgc tggtgacaca
    76621 tgctgcgttg tgtcaggata ttgacgcaga tttcatgtcg aggactacca tgaacaagtt
    76681 tttatttgcc gctgcattga tcgtcagtgg cctgctcgtt ggctgtaatc aactcaccca
    76741 atacaccatc accgaacaag aaattaacca gtcgcttgcg aaacataata atttctcaaa
    76801 agatatcggt ttacccggcg tggctgacgc ccatattgtt ctgacaaact tgaccagcca
    76861 aattggtcgc gaagagccga ataaggttac cctaaccgga gacgccaatc tggacatgaa
    76921 ctccctgttc ggtagtcaga aagcgaccat gaaactgaag ctgaaagcgc tgcccgtgtt
    76981 tgataaagaa aaaggtgcga tcttcctgaa agagatggaa gtggtcgatg cgacggtaca
    77041 accggaaaaa atgcaaacgg tgatgcaaac gttgcttccc tatttgaacc aggcattacg
    77101 caattacttt aaccagcaac ctgcttacgt cctgcgcgaa gatggcagtc agggcgaagc
    77161 aatggcgaaa aaactggcga aaggcattga agtgaagcca ggcgaaattg tcattccgtt
    77221 tacggattaa tgacgagggc gcattcgcgc cctttatttt tcgtgcaaag gaaaacattt
    77281 ccgcttatcc tttgtgtccg gcaaaaacat cccttcagcc ggagcataga gattaatgac
    77341 tgcaccatcc caggtattaa agatccgccg tccagacgac tggcaccttc acctccgcga
    77401 tggcgacatg ttacaaactg tcgtgccgta taccagcgaa atttatggac gggctatcgt
    77461 aatgcccaat ctggctccgc ccgtgaccac agttgaggct gccgtggcgt atcgccaacg
    77521 tattcttgac gccgtacctg ccgggcacga tttcacccca ttgatgacct gttatttaac
    77581 agattcgctg gatcctaatg agctggagcg cggatttaat gaaggcgtgt tcaccgctgc
    77641 aaaactttac ccggcaaacg caaccactaa ctccagccac ggcgtgacgt caattgacgc
    77701 aatcatgccg gtacttgagc gcatggaaaa aatcggtatg ccgctactgg tgcatggtga
    77761 agtgacacat gcagatatcg acatttttga tcgtgaagcg cgctttatag aaagcgtgat
    77821 ggaacctctg cgccagcgcc tgactgcgct gaaagtcgtt tttgagcaca tcaccaccaa
    77881 agatgctgcc gactatgtcc gtgacggaaa tgaacggctg gctgccacca tcactccgca
    77941 gcatctgatg tttaaccgca accatatgct ggttggtggc gtgcgtccgc acctgtattg
    78001 tttacccatc ctcaaacgca atattcacca acaggcattg cgtgaactgg tcgccagcgg
    78061 ttttaatcga gtattcctcg gtacggattc tgcgccacat acacgtcatc gcaaagagag
    78121 cagttgcggc tgcgcgggct gcttcaacgc cccaaccgcg ctgggcagtt acgctaccgt
    78181 ctttgaagag atgaatgctc tgcagcactt tgaagcattc tgttctgtaa acggcccgca
    78241 gttctatggc ttgccggtca acgacacatt catcgaactg gtacgtgaag agcaacaggt
    78301 tgctgaaagc atcgcactga ctgatgacac cctggtgcca ttcctcgccg gggaaacggt
    78361 acgctggtcc gttaaacaat aaaaatcgta gcttcctgtt gtcattaggt tattttacct
    78421 gtataaataa ccagtatatt caacaggggg ctattatgcg aattgaagtc accatagcga
    78481 aaacttctcc attgccagct ggggctattg acgccctggc tggcgaactt tcccgccgta
    78541 ttcagtatgc gtttcctgat aatgaaggcc acgtatcggt acgttatgcc gcagcgaata
    78601 atttatcggt tattggcgca acaaaagaag ataaacagcg cattagcgaa attctccagg
    78661 aaacgtggga aagcgccgat gactggtttg tcagcgaata atatgcagtg atttttgttt
    78721 ttgccgggtc gccccggctt ttttatttta cttacgaatt ttgagtacaa aatttctaca
    78781 cttcctcaaa ataattgcac aatatgctaa aaaaatatga atgacatgct gttttattgt
    78841 tcatataaat cttaaaagca gaaatttatc tgttcccccc tttaaaaatt cggtgaatac
    78901 ccttacttat tggtatactg aagttgcttc agataaaaac gcattgaacc tcgaataacg
    78961 ttgtctagta acacgaatta gggggccatg atggaaaaaa ataatgaagt cattcagact
    79021 catccgctcg tagggtggga catcagcacc gttgatagct atgatgcgct gatgttgcgt
    79081 ttgcactacc agaccccaaa taagtccgag caggaaggga ctgaagttgg tcagacgctc
    79141 tggttaacca ctgatgttgc cagacaattt atttcgatat tagaagcagg aatcgccaaa
    79201 attgaatccg gtgatttccc ggtaaacgag tatcggcgtc attaacatga catgaccatc
    79261 cacccaaaag gcacctcacc ggtgccttta ccatttctgg gttgtatatc tgcattcagt
    79321 taattactct gcttaaacca tgagagcaga gagaaaaaaa tgaaatacga tctcatcatt
    79381 attggcagcg gttccgtagg cgctgccgcc gggtattatg caacccgcgc cggtttaaac
    79441 gtgctaatga ccgacgccca tatgccaccg catcaacacg gcagccacca cggcgatacg
    79501 cgattaattc gccatgctta tggtgaaggc gaaaagtatg tcccgttagt cctccgcgcg
    79561 caaacgctgt gggatgaact ttcccgccac aacgaagatg atcccatttt tgtacgctct
    79621 ggtgtcatta acctcggccc ggctgactcc gcatttctcg ccaacgtcgc ccacagcgcc
    79681 gaacagtggc aactcaacgt tgaaaagctc gatgcgcaag ggattatggc ccgctggcca
    79741 gaaatacgcg tcccggacaa ctacatcggc ttatttgaga ctgattccgg ttttttgcgc
    79801 agcgaactgg cgattaaaac ctggatccaa ctggcgaagg aagcgggctg tgcgcaactg
    79861 ttcaactgcc cggtcaccgc aattcgtcat gacgatgatg gcgtaactat tgaaacggtt
    79921 gacggtgagt atcaggcgaa aaaagcgatt gtctgcgcgg gaacatgggt aaaagacctg
    79981 ctcccggagc tgcctgtcca gcccgtacgt aaagtatttg cctggtatca ggccgatggc
    80041 cgctatagcg tgaagaataa attcccggcg tttaccggtg aactgcccaa tggcgatcaa
    80101 tattatggtt ttccggcaga aaacgacgcg ttgaagattg gcaaacataa cggaggccag
    80161 gttatccatt cagcggatga acgtgttccg tttgcggaag tggccagtga tgggtcagaa
    80221 gccttcccgt tcttgcgcaa tgtattgccg ggtatcggtt gctgcctgta cggcgctgcc
    80281 tgcacctatg ataattcgcc tgacgaagat tttattatcg ataccctacc cggccacgat
    80341 aatacactgc tcattaccgg cctgagtggg cacggtttta aatttgcgtc agttttaggg
    80401 gaaatagctg ccgattttgc gcaagaccaa aaaagcgatt ttgatttgac gccattcagg
    80461 ctttcccgct tccaataatc atcattacgg cctccagaat gaggccgttt ttacaggagt
    80521 atttaatgcg tcggctgttg cactatctca tcaataatat tcgcgagcat ctgatgcttt
    80581 atctttttct ctggggattg ttggcaatta tggatctgat ctatgtgttt tatttttagt
    80641 tttccgcact gacataaact gacaaatatt taatctccct ttcatttagc gtgaaaaatt
    80701 gaatgagatt attcctgctt ctacagcact caccagacat ctctgatgct gaacccacta
    80761 aacttatgaa gattctttca ttattaattc aaaattattg aaataaaaat aaatatcatt
    80821 caatttgtta tttttgtgtt gcaattcatt ctccgacacg ccattttctt tctctcgcaa
    80881 attcgtattg gatgcactta tgtcattcac aaatacccct gaacgctatg gtgttatatc
    80941 tgcggccttc cattggctaa gcgcaattat tgtctacggc atgtttgctt taggtctgtg
    81001 gatggtcacg ctcagttatt acgatggctg gtatcacaaa gcacccgaac tgcataaaag
    81061 catcggtatt ttgttaatga tggggctggt tattcgcgtt ctgtggcgtg tcatatctcc
    81121 accgccgggt ccgctgccga gttattcgcc catgactcgt cttgccgcaa aagctggaca
    81181 tctcgcattg tacctattgc tgttcgccat cggcatcagc ggctatctga tctcaactgc
    81241 cgatggtaaa ccgatcagcg tttttggctg gtttgacgtc cccgcgaccc ttgccgacgc
    81301 tggcgcacag gcagactttg ccggtgccct gcatttttgg ctcgcgtgga gcgtcgtagt
    81361 actgtctgtt atgcacggat ttatggccct gaagcatcat ttcatcgata aagacgacac
    81421 tctgaagcgc atgctgggaa agtcgtcatc tgactatgga gtataaaaaa tgaaaaaaag
    81481 cctgcttggt ttaaccttcg cgtccctgat gttctctgcc ggttcagcgg ttgccgccga
    81541 ttacaaaatt gacaaagaag gtcagcacgc ctttgttaac ttccgcatac agcatcttgg
    81601 ctatagctgg ttatatggca cctttaaaga tttcgacggt acttttacct ttgatgaaaa
    81661 aaatccggct gccgataaag tgaatgtgac aattaacacc accagcgtcg atactaacca
    81721 cgccgaacgc gataaacatc ttcgcagtgc ggattttctc aataccacaa aatatccaca
    81781 ggcaacattc acctccacca gcgtgaagaa agacggtgac gaactggata ttaccggcga
    81841 tctgacgctg aatggcgtaa ccaaacccgt cacgctggaa gcgaaattaa ttggtcaggg
    81901 cgacgaccca tggggcggta aacgtgcagg ctttgaggcc gaaggcaaaa ttaagctcaa
    81961 agacttcaat atcaagacag atttaggtcc agcttctcag gaagtggatc tgattatttc
    82021 agtggaaggc gtacagcaga agtaagagtg aaatgttgcc ggatggcacg catccggcaa
    82081 tgatatttat tctgttggat caggaatacc cagtgttgta ttcagacgtc cacgagactt
    82141 attaaagatc ttattgccat tttcacgtcc tgcccgacgg cgtcgctgtt cttctggcgg
    82201 taacgcgctt tcttcgcagc aaatctcact acaacaacct ttgtattttt ccgcgcatac
    82261 tggacactga ataaacagca ggtggcagcc atcattttta cagttggtat ggctgtcgca
    82321 cggcgcgccg cactggtggc aatgcgcgat aatctcatcc gatatacgtt cgcccatccg
    82381 ctcgtcaaaa acaaaatttt tgccaataaa acgcaccggc aagccctgct cgcgcgcctt
    82441 acgggcgtat tcaataattc cgccctcgat atgccagact ttgttgaatc cgttatgttt
    82501 catccaggca ctggcctttt cacaacgaat gccgccggtg cagtacatga cgattttttt
    82561 atctttatgt gcctgcatca tctcgactgc ttttggcagc tgctcacgga aggtatctgc
    82621 cggaatttcg agcgcgtttt caaagtgccc cacttcatac tcatagtggt tacgcatgtc
    82681 gataaatagt gcatcgggat cgtcaagcat ggcgttcact tccgccgctt gcagatactc
    82741 accaacattg ctggcatcaa agtgaggatc gtcaataccg tcggcaacaa tgcgatcgcg
    82801 taccttcatg cgcagtaccc agaaggattt cccgtcatca tccaacgcga tattcaggcg
    82861 taaaccctct aaagccgggt cgaaggcata gagctgcgcg cgaaatgttt caacattgct
    82921 cgcaggtacg ctgatttgcg cgttaatgcc ctcatgcgcc agatacactc gcccaaaaac
    82981 attcagcgcg gtaaacagct gatataaagc gtcacgggtc gccttaggat cggcgatgtg
    83041 gaaatacttg taaaacgaaa tggtggttcg cggttcgctc tcagccaaca ttttggcttt
    83101 taacgcgtcg ttggaaatgc ggttgtgtaa cactggcatg gtgtacggtt cctgcgagat
    83161 gggaaagtaa aaatccgcgg catgatatag caattatcga taattaacat ccacacattt
    83221 tacgctacat ttgcgcatta aaaattattt gttatttaca agcgcggcaa tttcgcccag
    83281 tcttcagcca caaattttgg ttgcgggcga aaaaatgcga caatacatac aattgcccgt
    83341 ataggttgaa aaacaggatt gatatgacga atctacccaa gttctccacc gcactgcttc
    83401 atccgcgtta ttggttaacc tggttgggta ttggcgtact ttggttggtc gtgcaattgc
    83461 cctacccgat tatctaccgc ctcggttgtg gattaggaaa actggcgtta cgttttatga
    83521 aacgacgcgc aaaaattgtg catcgcaacc tggaactgtg cttcccggaa atgagcgaac
    83581 aagaacgccg taaaatggtg gtgaagaatt tcgaatccgt tggcatgggc ctgatggaaa
    83641 ccggtatggc gtggttctgg ccggaccgcc gaatcgcccg ctggacggaa gtgatcggca
    83701 tggaacacat tcgtgacgtg caggcgcaaa aacgcggcat cctgttagtt ggcatccatt
    83761 ttctgacact ggagctgggc gcgcggcagt ttggtatgca ggaaccgggg attggcgttt
    83821 accgcccgaa cgataatcca ctgattgact ggctacaaac ctggggccgt ttgcgctcaa
    83881 ataaatcgat gctcgaccgc aaagatttaa aaggcatgat taaagccctg aaaaaaggcg
    83941 aagtggtctg gtacgcacca gatcatgatt acggcccgcg ctcaagcgtt ttcgtcccgt
    84001 tatttgccgt tgagcaggct gcgaccacga ccggaagctg gatgctggca cggatgtccg
    84061 gcgcatgtct ggtgcccttc gttccacgcc gtaagccaga tggcaaaggg tatcaattga
    84121 ttatgctgcc gccagagtgt tctccgccac tggatgatgc cgaaactacc gccgcgtgga
    84181 tgaacaaagt ggtcgaaaaa tgcatcatga tggcaccaga gcagtatatg tggttacacc
    84241 gtcgctttaa aacacgcccg gaaggcgttc cttcacgcta ttaaatctcc catgccggat
    84301 gcttcagaat ggcatccggc attaccacag caaatccccc tgatttagcg ataaaagctc
    84361 tctggattgc gccccctgga agtcgggcgc ataattagtg tgtttatctt ttcttcttat
    84421 gttcaccgcg cctggcgcac caacagcgga ttgctatgtc accctgtgaa aatgacaccc
    84481 ctataaactg gaaacgaaac ctgatcgtcg cctggctagg ctgttttctt accggggccg
    84541 ccttcagtct ggtaatgccc ttcttacccc tctacgttga gcagcttggt gttaccggcc
    84601 actccgccct gaatatgtgg tccggtattg tcttcagtat aatgggttcc taaacacatc
    84661 attttagata ttgaatatga gacgggcaat cagctacatc agattttcaa gtgaacgaca
    84721 actgaaaggg gactctgtac gccgacaatc taagctggtt actgactggc ttgataagaa
    84781 ccctgagttt tactgggatt catccttgag ctttaaagat ttggggaaaa gtgcttttag
    84841 tggtaagcac ctgaaaggtg gtctaggtga ctttttaacc gctatcgaga aagggcttgt
    84901 taaagctggt gatacgctac taatcgagag tttagaccgt ctgtcacgtc aggatattga
    84961 tattgcttct gagctattac gccgtatctt aagggctggt gtagatgtag tcacactgag
    85021 cgatggagaa cattacacac gagaaagttt aaaagacccg ttagccctga ttaaaagcat
    85081 tctgataatg cagcgggcgc atgaggaaat tttgaggaag tctgagcgtg tccaagctgc
    85141 atggaaccgt aaaaaagaac tcatctccga aggtatcaag gtatctaggc gttgtcctgc
    85201 atggttaagg ctgaatgatg accgtcgaac attcacaatc attcctgata aagtggaagt
    85261 tgttaaacga gcatttgacc tgagattaca gggtttaagc ttttgggcta tcaccagaac
    85321 tttaaacgat gaaggtcact tgtcattgaa tcaatacaca cccaaacaaa aggggtggtc
    85381 tgatactgct gtaaagaaac tactcagaaa tcgtgctgtg attggatgtt tcacacctgc
    85441 tggacgtgaa gaagttcagg gttactaccc cgcaataatc agtgagtcac tattctatcg
    85501 agtacagcaa ctgaacacag ggcaatatgg tagggcttct gtatcaagta atccgctatc
    85561 agtcaacttg ttcaggggta tcatcaaatg ttctg
//
LOCUS       DADMEQ010000003        82541 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.003, whole genome shotgun sequence.
ACCESSION   DADMEQ010000003 DADMEQ010000000
VERSION     DADMEQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 82541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.003"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..372)
                     /locus_tag="JNZ05_000186"
     CDS             complement(<1..372)
                     /locus_tag="JNZ05_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY8563550.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGR"
     gene            complement(443..2557)
                     /gene="fusA"
                     /locus_tag="JNZ05_000187"
     CDS             complement(443..2557)
                     /gene="fusA"
                     /locus_tag="JNZ05_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAY8563551.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRIGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2654..3124)
                     /gene="rpsG"
                     /locus_tag="JNZ05_000188"
     CDS             complement(2654..3124)
                     /gene="rpsG"
                     /locus_tag="JNZ05_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAY8563552.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3221..3595)
                     /gene="rpsL"
                     /locus_tag="JNZ05_000189"
     CDS             complement(3221..3595)
                     /gene="rpsL"
                     /locus_tag="JNZ05_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003852912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAY8563553.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3721..4008)
                     /gene="tusB"
                     /locus_tag="JNZ05_000190"
     CDS             complement(3721..4008)
                     /gene="tusB"
                     /locus_tag="JNZ05_000190"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HAY8563554.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4016..4375)
                     /gene="tusC"
                     /locus_tag="JNZ05_000191"
     CDS             complement(4016..4375)
                     /gene="tusC"
                     /locus_tag="JNZ05_000191"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HAY8563555.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4375..4761)
                     /gene="tusD"
                     /locus_tag="JNZ05_000192"
     CDS             complement(4375..4761)
                     /gene="tusD"
                     /locus_tag="JNZ05_000192"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HAY8563556.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4761..5483)
                     /locus_tag="JNZ05_000193"
     CDS             complement(4761..5483)
                     /locus_tag="JNZ05_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8563557.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5650..6462)
                     /gene="fkpA"
                     /locus_tag="JNZ05_000194"
     CDS             complement(5650..6462)
                     /gene="fkpA"
                     /locus_tag="JNZ05_000194"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003827768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY8563558.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6683..6901
                     /locus_tag="JNZ05_000195"
     CDS             6683..6901
                     /locus_tag="JNZ05_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HAY8563559.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6950..7540)
                     /gene="slyD"
                     /locus_tag="JNZ05_000196"
     CDS             complement(6950..7540)
                     /gene="slyD"
                     /locus_tag="JNZ05_000196"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY8563560.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7635..7835)
                     /locus_tag="JNZ05_000197"
     CDS             complement(7635..7835)
                     /locus_tag="JNZ05_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HAY8563561.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7845..9608)
                     /gene="kefB"
                     /locus_tag="JNZ05_000198"
     CDS             complement(7845..9608)
                     /gene="kefB"
                     /locus_tag="JNZ05_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005116068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HAY8563562.1"
                     /translation="MFYSQECCFSSRLGIGAVLGYLLAGIAIGPWGLGFISDVDEILH
                     FSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGVAQVLLSAALLAGLLMLTDFAWQAA
                     VVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAVIPALALVPLLAGSA
                     DEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFTAATLLLVLGSALFM
                     DALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFISVGMSLNLGVLYTH
                     LLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGGEFAFVLFSTASSQR
                     LFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDEKPWVNDDKPQVIVV
                     GFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGDATQVDLLRSAGAEA
                     AESIVITCNEPEDTMKLVEICQQHFPHLHILARVRGRVEAHELLQAGVTQFSRETFSS
                     ALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADTVQISRAREARRELE
                     EIFQREMQQERRQLDGWDEFE"
     gene            complement(9621..10172)
                     /gene="kefG"
                     /locus_tag="JNZ05_000199"
     CDS             complement(9621..10172)
                     /gene="kefG"
                     /locus_tag="JNZ05_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HAY8563563.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSSIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10303..12216
                     /locus_tag="JNZ05_000200"
     CDS             10303..12216
                     /locus_tag="JNZ05_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8563564.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIQ
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQAVTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANNAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12216..13238
                     /locus_tag="JNZ05_000201"
     CDS             12216..13238
                     /locus_tag="JNZ05_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY8563565.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYLLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13232..13450
                     /locus_tag="JNZ05_000202"
     CDS             13232..13450
                     /locus_tag="JNZ05_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HAY8563566.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13504..14373
                     /locus_tag="JNZ05_000203"
     CDS             13504..14373
                     /locus_tag="JNZ05_000203"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HAY8563567.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     LLVQRLMEGKKIE"
     gene            complement(14428..14832)
                     /locus_tag="JNZ05_000204"
     CDS             complement(14428..14832)
                     /locus_tag="JNZ05_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HAY8563568.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15134..15766
                     /gene="crp"
                     /locus_tag="JNZ05_000205"
     CDS             15134..15766
                     /gene="crp"
                     /locus_tag="JNZ05_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HAY8563569.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15817..17907
                     /locus_tag="JNZ05_000206"
     CDS             15817..17907
                     /locus_tag="JNZ05_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8563570.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17974..19194)
                     /gene="argD/dapC"
                     /locus_tag="JNZ05_000207"
     CDS             complement(17974..19194)
                     /gene="argD/dapC"
                     /locus_tag="JNZ05_000207"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HAY8563571.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPQPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQVLLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19280..19843)
                     /gene="pabA"
                     /locus_tag="JNZ05_000208"
     CDS             complement(19280..19843)
                     /gene="pabA"
                     /locus_tag="JNZ05_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HAY8563572.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19875..20477)
                     /locus_tag="JNZ05_000209"
     CDS             complement(19875..20477)
                     /locus_tag="JNZ05_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HAY8563573.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEIDVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20467..20634)
                     /locus_tag="JNZ05_000210"
     CDS             complement(20467..20634)
                     /locus_tag="JNZ05_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HAY8563574.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20739..21311)
                     /gene="ppiA"
                     /locus_tag="JNZ05_000211"
                     /gene_synonym="rotA"
     CDS             complement(20739..21311)
                     /gene="ppiA"
                     /locus_tag="JNZ05_000211"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HAY8563575.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21582..22763
                     /gene="tsgA"
                     /locus_tag="JNZ05_000212"
     CDS             21582..22763
                     /gene="tsgA"
                     /locus_tag="JNZ05_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HAY8563576.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFSCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23025..25568
                     /locus_tag="JNZ05_000213"
     CDS             23025..25568
                     /locus_tag="JNZ05_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HAY8563577.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHSSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIARKFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25565..25891
                     /gene="nirD"
                     /locus_tag="JNZ05_000214"
     CDS             25565..25891
                     /gene="nirD"
                     /locus_tag="JNZ05_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HAY8563578.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26017..26823
                     /gene="nirC"
                     /locus_tag="JNZ05_000215"
     CDS             26017..26823
                     /gene="nirC"
                     /locus_tag="JNZ05_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HAY8563579.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26842..28215
                     /gene="cobA"
                     /locus_tag="JNZ05_000216"
     CDS             26842..28215
                     /gene="cobA"
                     /locus_tag="JNZ05_000216"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY8563580.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28462..28629
                     /locus_tag="JNZ05_000217"
     CDS             28462..28629
                     /locus_tag="JNZ05_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HAY8563581.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28924..30261
                     /gene="frlA"
                     /locus_tag="JNZ05_000218"
                     /pseudo
     CDS             28924..30261
                     /gene="frlA"
                     /locus_tag="JNZ05_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30282..31304
                     /gene="frlB"
                     /locus_tag="JNZ05_000219"
     CDS             30282..31304
                     /gene="frlB"
                     /locus_tag="JNZ05_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HAY8563582.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31354..32183
                     /gene="frlC"
                     /locus_tag="JNZ05_000220"
                     /pseudo
     CDS             31354..32183
                     /gene="frlC"
                     /locus_tag="JNZ05_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            32180..32965
                     /gene="frlD"
                     /locus_tag="JNZ05_000221"
     CDS             32180..32965
                     /gene="frlD"
                     /locus_tag="JNZ05_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAY8563583.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            33065..33796
                     /gene="phoB"
                     /locus_tag="JNZ05_000222"
     CDS             33065..33796
                     /gene="phoB"
                     /locus_tag="JNZ05_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HAY8563584.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33956..35041)
                     /locus_tag="JNZ05_000223"
     CDS             complement(33956..35041)
                     /locus_tag="JNZ05_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY8563585.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLLALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35053..36357)
                     /locus_tag="JNZ05_000224"
     CDS             complement(35053..36357)
                     /locus_tag="JNZ05_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8563586.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHLILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTWLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36369..36722)
                     /locus_tag="JNZ05_000225"
     CDS             complement(36369..36722)
                     /locus_tag="JNZ05_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HAY8563587.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36733..37611)
                     /locus_tag="JNZ05_000226"
     CDS             complement(36733..37611)
                     /locus_tag="JNZ05_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HAY8563588.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLCQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37608..38834)
                     /locus_tag="JNZ05_000227"
     CDS             complement(37608..38834)
                     /locus_tag="JNZ05_000227"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY8563589.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38835..39998)
                     /locus_tag="JNZ05_000228"
     CDS             complement(38835..39998)
                     /locus_tag="JNZ05_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HAY8563590.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLKA"
     gene            complement(40082..40444)
                     /locus_tag="JNZ05_000229"
     CDS             complement(40082..40444)
                     /locus_tag="JNZ05_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY8563591.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40461..41366)
                     /locus_tag="JNZ05_000230"
     CDS             complement(40461..41366)
                     /locus_tag="JNZ05_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8563592.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLLATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41636..42640)
                     /gene="trpS"
                     /locus_tag="JNZ05_000231"
     CDS             complement(41636..42640)
                     /gene="trpS"
                     /locus_tag="JNZ05_000231"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAY8563593.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42633..43391)
                     /gene="gph"
                     /locus_tag="JNZ05_000232"
     CDS             complement(42633..43391)
                     /gene="gph"
                     /locus_tag="JNZ05_000232"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HAY8563594.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43384..44061)
                     /gene="rpe"
                     /locus_tag="JNZ05_000233"
     CDS             complement(43384..44061)
                     /gene="rpe"
                     /locus_tag="JNZ05_000233"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAY8563595.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(44079..44915)
                     /gene="dam"
                     /locus_tag="JNZ05_000234"
     CDS             complement(44079..44915)
                     /gene="dam"
                     /locus_tag="JNZ05_000234"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY8563596.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(45022..46308)
                     /gene="damX"
                     /locus_tag="JNZ05_000235"
     CDS             complement(45022..46308)
                     /gene="damX"
                     /locus_tag="JNZ05_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HAY8563597.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46400..47488)
                     /gene="aroB"
                     /locus_tag="JNZ05_000236"
     CDS             complement(46400..47488)
                     /gene="aroB"
                     /locus_tag="JNZ05_000236"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAY8563598.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47545..48066)
                     /gene="aroK"
                     /locus_tag="JNZ05_000237"
     CDS             complement(47545..48066)
                     /gene="aroK"
                     /locus_tag="JNZ05_000237"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HAY8563599.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQKLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48467..49705)
                     /gene="hofQ"
                     /locus_tag="JNZ05_000238"
     CDS             complement(48467..49705)
                     /gene="hofQ"
                     /locus_tag="JNZ05_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HAY8563600.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQASSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49617..50021)
                     /gene="hofP"
                     /locus_tag="JNZ05_000239"
     CDS             complement(49617..50021)
                     /gene="hofP"
                     /locus_tag="JNZ05_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HAY8563601.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(50011..50451)
                     /gene="hofO"
                     /locus_tag="JNZ05_000240"
     CDS             complement(50011..50451)
                     /gene="hofO"
                     /locus_tag="JNZ05_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HAY8563602.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50435..50974)
                     /locus_tag="JNZ05_000241"
     CDS             complement(50435..50974)
                     /locus_tag="JNZ05_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HAY8563603.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50974..51753)
                     /gene="hofM"
                     /locus_tag="JNZ05_000242"
     CDS             complement(50974..51753)
                     /gene="hofM"
                     /locus_tag="JNZ05_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HAY8563604.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51873..54425
                     /gene="mrcA"
                     /locus_tag="JNZ05_000243"
     CDS             51873..54425
                     /gene="mrcA"
                     /locus_tag="JNZ05_000243"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HAY8563605.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54590..55150)
                     /gene="nudE"
                     /locus_tag="JNZ05_000244"
     CDS             complement(54590..55150)
                     /gene="nudE"
                     /locus_tag="JNZ05_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HAY8563606.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55470..57605
                     /gene="igaA"
                     /locus_tag="JNZ05_000245"
     CDS             55470..57605
                     /gene="igaA"
                     /locus_tag="JNZ05_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HAY8563607.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRQRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLVLNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57670..58338
                     /gene="yrfG"
                     /locus_tag="JNZ05_000246"
     CDS             57670..58338
                     /gene="yrfG"
                     /locus_tag="JNZ05_000246"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HAY8563608.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58349..58750
                     /gene="hslR"
                     /locus_tag="JNZ05_000247"
     CDS             58349..58750
                     /gene="hslR"
                     /locus_tag="JNZ05_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HAY8563609.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIKGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58775..59653
                     /gene="hslO"
                     /locus_tag="JNZ05_000248"
     CDS             58775..59653
                     /gene="hslO"
                     /locus_tag="JNZ05_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAY8563610.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59788..61512)
                     /locus_tag="JNZ05_000249"
     CDS             complement(59788..61512)
                     /locus_tag="JNZ05_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HAY8563611.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61891..63513
                     /gene="pckA"
                     /locus_tag="JNZ05_000250"
     CDS             61891..63513
                     /gene="pckA"
                     /locus_tag="JNZ05_000250"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAY8563612.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63589..64941)
                     /gene="envZ"
                     /locus_tag="JNZ05_000251"
     CDS             complement(63589..64941)
                     /gene="envZ"
                     /locus_tag="JNZ05_000251"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HAY8563613.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64938..65657)
                     /gene="ompR"
                     /locus_tag="JNZ05_000252"
     CDS             complement(64938..65657)
                     /gene="ompR"
                     /locus_tag="JNZ05_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HAY8563614.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65849..66361
                     /gene="greB"
                     /locus_tag="JNZ05_000253"
     CDS             65849..66361
                     /gene="greB"
                     /locus_tag="JNZ05_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HAY8563615.1"
                     /translation="MRIIKQTKGINEMKTPLVTREGYEKLKQELNYLWREERPEVTKK
                     VTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAW
                     VEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANW
                     YVNAIEYVKP"
     gene            66458..68779
                     /locus_tag="JNZ05_000254"
     CDS             66458..68779
                     /locus_tag="JNZ05_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAY8563616.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVAAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNGVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69217..69444
                     /gene="feoA"
                     /locus_tag="JNZ05_000255"
     CDS             69217..69444
                     /gene="feoA"
                     /locus_tag="JNZ05_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HAY8563617.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69461..71782
                     /gene="feoB"
                     /locus_tag="JNZ05_000256"
     CDS             69461..71782
                     /gene="feoB"
                     /locus_tag="JNZ05_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HAY8563618.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71782..72018
                     /gene="feoC"
                     /locus_tag="JNZ05_000257"
     CDS             71782..72018
                     /gene="feoC"
                     /locus_tag="JNZ05_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HAY8563619.1"
                     /translation="MASLIQVRDLLALRCRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72221..73099
                     /gene="rpnA"
                     /locus_tag="JNZ05_000258"
     CDS             72221..73099
                     /gene="rpnA"
                     /locus_tag="JNZ05_000258"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HAY8563620.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            73172..73441
                     /locus_tag="JNZ05_000259"
     CDS             73172..73441
                     /locus_tag="JNZ05_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HAY8563621.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            73435..73800
                     /locus_tag="JNZ05_000260"
     CDS             73435..73800
                     /locus_tag="JNZ05_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAY8563622.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPCDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(73801..74589)
                     /gene="bioH"
                     /locus_tag="JNZ05_000261"
     CDS             complement(73801..74589)
                     /gene="bioH"
                     /locus_tag="JNZ05_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HAY8563623.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPYFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            74627..75310
                     /gene="gntX"
                     /locus_tag="JNZ05_000262"
     CDS             74627..75310
                     /gene="gntX"
                     /locus_tag="JNZ05_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HAY8563624.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            75369..75944
                     /gene="nfuA"
                     /locus_tag="JNZ05_000263"
     CDS             75369..75944
                     /gene="nfuA"
                     /locus_tag="JNZ05_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HAY8563625.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            76305..77609
                     /gene="gntT"
                     /locus_tag="JNZ05_000264"
     CDS             76305..77609
                     /gene="gntT"
                     /locus_tag="JNZ05_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY8563626.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLANCGGAQRIATTLIAKFGKKHIQWAVVLTGF
                     TVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGPTAIA
                     TIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEEMPSF
                     GVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLN
                     RGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNISPLL
                     MAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDP
                     GFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(77654..79738)
                     /gene="malQ"
                     /locus_tag="JNZ05_000265"
     CDS             complement(77654..79738)
                     /gene="malQ"
                     /locus_tag="JNZ05_000265"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HAY8563627.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(79748..82141)
                     /gene="malP"
                     /locus_tag="JNZ05_000266"
     CDS             complement(79748..82141)
                     /gene="malP"
                     /locus_tag="JNZ05_000266"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HAY8563628.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        19786 a        22411 c        20833 g        19511 t
ORIGIN      
        1 acgacccagc aggatgtgct cacgagtctg cggcatcggg ccgtcagtcg cagcaactac
       61 caggatcgcg ccgtccatct gagcagcacc ggtgatcatg tttttaacat agtcggcgtg
      121 ccccgggcag tctacgtgtg cgtagtgacg ggtcggggtg tcatattcaa cgtgagaagt
      181 gttgatggtg ataccacgag ctttttcttc cggcgcgtta tcgatctggt cgaatgcacg
      241 agcagcaccg ccgtaggttt tagccaatac ggtggtgatt gcagcggtca gagtagtttt
      301 accgtggtca acgtggccga tagtaccaac gttaacgtgc ggttttgtac gttcaaattt
      361 ttctttagac acggctatat tccttactat agtgctctcc ccttcaggag agagcacggg
      421 actttggtat taacccttag gcttatttac cacgggcttc aattacggcc tgagcaacgt
      481 tactcggcgc ttcatcatac ttcaggaatt ccatagtgta tgatgcacga cctttggtca
      541 gagaacgcag ctgagttgcg tatccgaaca tttcagacag cggtacttca gcgtggatct
      601 taacgccagt aacttcagat tcctgacctt tgagcatacc acgacgacgg ctcaagtcac
      661 cgataacgtc accggtgttc tcttccggag tttctacttc aaccttcatg atcggctcaa
      721 gcagaactgg tttcgctttc ttaaagcctt ctttaaaggc gatagaagca gccagtttaa
      781 acgccagttc agaggagtca acgtcatggt aagaaccgaa gtgcagacga atacccatgt
      841 ctactaccgg gtagcctgcc agcggacctg ctttcagctg ttcctggata cctttatcaa
      901 cggccgggat gtattcgcca gggattacac cacctttaat gtcgttgatg aactcgtagc
      961 ctttcgggtt tgaacccggc tccagcgggt acatgtcgat aacaacatga ccatactgac
     1021 cacgaccacc agactgtttc gcgtgtttac cttcaacatc ggtaactttc tggcggatag
     1081 tttcacggta agcaacctgc ggtttaccta cgttcgcttc aacgttgaat tcacgcttca
     1141 tacggtcaac gatgatgtcg aggtgcagtt cgcccatacc cgcgatgatg gtctggttag
     1201 attcttcgtc agtccataca cggaaagacg ggtcttcttt agccagacgg cccagagcca
     1261 gacccatttt ttcctggtca gctttggttt tcggttcaac tgcgatggag attaccggct
     1321 cagggaattc catacgttcc agaatgatcg gcgcatccgg gtcacacagg gtgtcaccag
     1381 tggttacgtc tttcagaccg atagcagcag cgatgtcgcc cgcgcgaact tctttgatct
     1441 cttcacgttt gttagcgtgc atctgaacga tacgaccgaa acgctcacgt gcagctttca
     1501 cggagttcag tacggtatca ccagagttaa ccacaccgga gtaaacacgg aagaaggtca
     1561 ggttaccaac aaacgggtcg gtagcgattt tgaacgccag tgcagagaac ggctcgtcat
     1621 cacttgcgtg acgttcagcc ggagtgtctt taccgtcgtc caggataccg ttgatcgcag
     1681 gtacgtcaac cggggatggc aggtaatcaa ttaccgcatc cagcatcgcc tgaacacctt
     1741 tgttcttgaa cgcagaacca caggttacca ggatgatttc gttgttcaga acgcgctgac
     1801 gcagagcacc tttgatttct gcttcagtca gttcttcacc acccaggtat ttttccatca
     1861 gctcttcaga agcttcagct gcggattcga tcaggttctg gtgccattcg ttagccagtt
     1921 caaccatgtc tgccgggata tcttcgtatt cgaaggttac gccctggtca gcgtcgttcc
     1981 agttgatagc tttcattttc accaggtcaa caacaccggt gaaatgttct tcagcaccaa
     2041 tcgccagctg cagcggaacc gggttcgcgc ccagacgggt tttgatctgg ttaacaactt
     2101 tcaggaagtt cgcacctatg cggtccattt tgttaacgaa cgcaatgcgc ggaactttat
     2161 atttgtttgc ctgacgccat acggtttcag actgcggctg aacaccacca actgcgcagt
     2221 aaaccattac cgcaccatcg agaacacgca tggaacgttc tacttcgatt gtgaagtcaa
     2281 cgtgccccgg ggtgtcgatg atgttgatgc gatgcggctc atactgctta gccataccag
     2341 accagaatgc agtagtcgca gcggaagtga tggtaatacc acgttcctgc tcctgctcca
     2401 tccagtccat ggttgcagcg ccgtcatgaa cttcaccgat tttatggttt acaccggtgt
     2461 agaacagaat acgttcggta gtagtggttt taccggcgtc gatgtgcgca ctgataccga
     2521 tgttacggta gcgtgcgatg ggtgttgtac gagccatttg tttcctcgtt tatcttttag
     2581 gcgttcaatt taagtagccc aaagcgggct gcttactgga agcgcccgcc tggtgactaa
     2641 aactccgaag ggattaccaa cggtagtgtg cgaacgcctt gttggcttcg gccatacggt
     2701 gaacgtcttc acgtttctta actgcagtac ctttgttttc tgcagcatca gaaagttcgt
     2761 tcgccaggcg cagagccatg gatttatcac cgcgtttacg agcagcttca acgatccaac
     2821 gcattgccag agcattacga cgaaccggac ggacttcaac tggtacctga taagtagaac
     2881 caccaacgcg gcgagactta acttctacag tcgggcgcac gttttcgaga gctacttcga
     2941 atgcttccag ttcagattta ccagagcgct gagccagggt ctccagcgcg ctgtatacga
     3001 tagattcagc agtagatttt ttaccatcta ccatcaggat atttacaaat ttagccagca
     3061 gttctgatcc gaacttcgga tccggcagaa ttttacgctg accaatgacg cgacgacgtg
     3121 gcatggaaat actccgttgt taattcagga ttgtccaaaa ctctacgagt ttagtttgac
     3181 atttaagtta aaacgtttgg ccttacttaa cggagaacca ttaagcctta ggacgcttca
     3241 cgccatactt ggaacgagcc tgcttacggt ctttaacgcc ggagcagtca agcgcaccac
     3301 gtacggtgtg gtaacgaaca cccgggaggt ctttaacacg accgccacgg atcaggatca
     3361 cggagtgctc ctgcaggttg tgaccttcac caccgatgta ggaagtcact tcgaaaccgt
     3421 tagtcagacg aacacggcat actttacgca gcgcggagtt cggtttttta ggagtggtag
     3481 tatatacacg agtacatacg ccacgttttt gcgggcatgc ttccagcgca ggcacgttgc
     3541 ttttcgcaac tttgcgagca cgtggtttgc gtaccagctg gttaactgtt gccattaaat
     3601 agctcctggt tttagctttt gcttcgtaaa cacgtaataa aacgtcctca cacaatatga
     3661 ggacgccgaa ttttagagcg atgccgaaaa ggtgtcaaga aatatacaac gatcccgcca
     3721 tcaccaggcc atctggctgg agtgcttaac cgtaagtctg acgaaatcag tatagtcaat
     3781 gggaatgatg tcgttcaaaa tttgaccagt caaaccgcgg gcaataaggt cttcgttcag
     3841 ggcatagacc ttaatggggg cattacgcag actttcaagg tagcggttac cgtcaactgc
     3901 ggcagttacg ccatcttgca ataatagcag ttcgtctcct tcactgagca gacgcagcag
     3961 cgcagcaaaa tccgtcagcc agggtgaacg atgtaacgtg tgcagcataa acgcctcaaa
     4021 acctcaaaat aacatcgtag ttggcgagtt cgcggcgtaa ggcatctgct tcgagcggcg
     4081 tggcttcgac aacaaagggt gtctgcggat ctaacccgcg ttcgcgcagt gaagccgcac
     4141 aaacccagca ctgttcaatg tcgtacagac tcaacaattt aaaagtggca atgtaatcac
     4201 gcgccagcac tgcatcgggc ttttgtcctg acagcagctg aaaaacgcca tcagcaataa
     4261 agaagacggc cagatcgtca gttaatgcgg aagttgccag taaagcatct aaaccttccc
     4321 gaccagcagc tgtaccatga ggtgcagtag aaaaaacaaa cgcaattcgt ttcatcagaa
     4381 ctgtaccacc ctgtcacagg tcagcgaggc ttccgccagc gcgccaagtc cgcttaaggt
     4441 aaatcccggc tgaaggtttg acgaagccag ccccagcctt cctgcttcag tttcatcgac
     4501 gataccgcgg cgtaacgctg ccgctacgca gatattcagc gccacaccat gttgcgcatt
     4561 caactgttgc cagccgcgca caaggtcaaa ttcgtcgctt gccggtgagg tcagttggtt
     4621 agcgttatag accccttccc gatagaaaaa gacgctgctt aacttatggc cttctgctat
     4681 cagcgcctgc gcaaactgaa aagcactact cgcctgttgc gtaccgtatg ctggcccggt
     4741 caccacgatg gcaaaacgca ttacttatct tgcccctgaa aatcaccgct cttgaactgg
     4801 cggatgtaga gatagacagt gtgtttggag atgttcaggc ggtcagcaac ctggttgatc
     4861 gcatctttaa tatcgaagat ccctttctcg tagagattca gcacgatctg acgatttttg
     4921 gcgttattag aaacattgcg atcggcattc acttcttcga tggtgaactc cagcgtttgg
     4981 gtaaccagat cttcaacaga agaggcaaag ttgacgcttg aaccgacatc cggggtttct
     5041 ggcggcacaa aagtgctcat aatctgcgag aagggaacat caagattcat attgatgcac
     5101 agcagaccaa ttacacgctg ttcgcggtta cgaatcgcga tagtcaggga cttcattaat
     5161 acgccgcttt tggcgcgagt aaagtagcat ttagaaacgc tgctatccgc tcccgtcata
     5221 tcgtgcagca tacgtagcgc caggtcagta attggcgaac caatcttccg gcctgtatgt
     5281 tcaccgttag caatgcgaat ggctgaacat tttagatcct gcaaagagtg caaaacgatt
     5341 tcacagtggg agccaataag catcgctaac ccgtccacca ccgcttcgta ggatttcaga
     5401 atatcaaaat cggtctggtc gaaaggacgt tgatccagta aatccaactc actggtttcg
     5461 ttggttaaaa gcgacctgga catgaaaaaa aacactcctt ttcaggagcc tgtcgttaac
     5521 ttttcagggc aggctcatta atgatgcggg taactaaatt aatacagcgg aggttccgct
     5581 ttccagcact aattatatcc ggcctgtaat aaaaaaaccg ccgcctggtc aggcggcggt
     5641 tcttaatgct tattttttag cagaatctgc ggctttcgca tcagcttccg gctttgcatc
     5701 agccttcggc gctggtttca catccagcag ctctacgtca aacactaggg tagaattcgg
     5761 tgggatcccc ggaacacccg ctttgccgta agccagttct ggtggaataa ccagtttgat
     5821 cttaccgcct ttcttgatgt tcttcagacc ttctgtccaa cccgggataa caccgtccag
     5881 acggaaagaa agcggttcac cacgggtgta agagttgtcg aactctttac cgtcgatcag
     5941 cgtacctttg tagttcacta caacagtatc gctgtctttc ggtgcttcgc ctttaccggc
     6001 ttctactacc tgataaacca gaccagttga agaggttttc acacctttct ctttggcaaa
     6061 tttctcgcgg tactctttac cttttgcttc gttatcagcc gcgtcttttt ccatcttcgc
     6121 ctgagcagaa gacttcacgc gagcttcgaa tgcttgcaga gtctgttcga tctcttggtc
     6181 ggagagtttg ctcttatcag caaatgcatc ctgaacacca gcgatcagct gatctttatc
     6241 cagtttgatg cccagttttt cttgttcttt aagagagttt tccatgtaac gacccagcga
     6301 agcacccagt gcataagctg atttctgatc gtcatttttg aacgctgctt tgctgtcagc
     6361 agttgtagca ggttttgcag cttcagcagc aaaagtgatt ggtgcatgca gggcaacggc
     6421 cattgtggtc gccagtagcg ttactttaaa cagtgatttc atccatatct ccaggatcgg
     6481 ggcatctcac cccagggtta actattatca gaagggtact ataaagcgtt gtcgaacaaa
     6541 tctacataca gacacgccct attatcatct attttcagac tctttttgtt taaattagtt
     6601 tcgatgaccg cgaaatgagt gctgtctcgg gcagcaaagt taagtagaat ccgcggcgac
     6661 cattcgacaa aagaggtgaa tcatgcagga tttatcattg gaagcacgcc tggcagagct
     6721 ggagagccga ctggcttttc aggaaatcac cattgaagaa ctgaacgtca cggtgaccgc
     6781 tcatgaaatg gagatggcga aactgcgcga tcatctgcgt ctgctgaccg agaagttaaa
     6841 agccagccag ccgtcgaaca tcgcgtcgca ggctgaagaa acgccaccgc cacattattg
     6901 aggcgtaaaa aaagcgggga ttccccgctt ttttgtcacc ttttcggtat tagtggcaac
     6961 cacaaccgcc gttgccttta ccgccacagc agccttcgcc accgtgttca tgaccgtgat
     7021 cgtggccatg accgccgcag caaccgtcgt ggtcgtgatc gtggtgatga tcgtgcgcgc
     7081 cgtgaacgtg gccatgagcc agttcttctt cggtcgcttc gcgaatcgcc acaacttcaa
     7141 cgttgaattt caggttctga ccggccagca tgtggttacc atcaaccact acgtgatcgt
     7201 cttcaaccgc agtgatttca accggtaccg gaccctggtc agtttcagcc aggaaacgca
     7261 tacctacctg cagttcatca acgcccataa atacgtcttt aggaacacgt tgcactaggt
     7321 tttcgtcgta ctgaccgtaa gcgtcgttcg cgccaacagc gacatcaaat ttgtcgccaa
     7381 cttcatgacc ttccagcgcc gtttccaggc cagagatcag ggaaccgtga ccatgcaggt
     7441 agtccagcgg cgcactcacc ggagactcat caaccaacac accgtcttct gtacgtacct
     7501 gataggccag gctgaccacc aggtcttttg ctactttcat gatatctcct gagcatggga
     7561 agaatagtgg cgcagattgt agcggaattc tgcagccgcg tactcactag cttaaaaaaa
     7621 ctcggcgcat atcgctagtc cggatgaaaa atcccgatca cttgctcatc tttgtgaacg
     7681 tgatcgcggg cttctttgtc tgcttctcgc atctgatgtc cgcacttaac acattcaaca
     7741 atatcaatat tattttcgcg ccacatcgcc attgaatcct gcgcctgaca ggccgggcat
     7801 tttgcgcccg ctataaaacg ttttcggatt gccatcctta ccctctactc aaattcatcc
     7861 cagccgtcca gctggcgtcg ttcttgttgc atttcacgct ggaaaatctc ttccagttcg
     7921 cgtcgggctt ccctggcgcg agaaatttgt acggtatcag catgcatcgg gattaactcc
     7981 cgcagcattc gcatatccag gcggcgaaaa tgcagttgcg cccgctgtgc ctgatgcgga
     8041 tgcatgccaa gcgtgaccag cgtcttgcgc cccagctcta acgcactgga gaatgtttca
     8101 cgggaaaact gcgtcacccc tgcctgtaat aactcatgcg cttccacacg tccgcgcact
     8161 cgcgcaagaa tatgcaaatg cggaaagtgc tgctggcata tttccaccag cttcatggtg
     8221 tcttccggct cgttacaggt aatgacgata gactcagcgg cctctgcacc cgcagaacgt
     8281 aaaagatcga cctgcgtggc gtcgccgtaa taaactttgt agccatattt acgcatcagg
     8341 ttaacggcgc tgatatcccg ctccagcacg gtaatgcgca ttttatttgc catcagcaaa
     8401 cgaccaatca cctgaccaaa acgcccgaag cccacgacaa tgacctgggg tttatcatcg
     8461 ttgacccacg gtttttcgtc ttcttcttcc ggtccgttaa actggcggga tagccattta
     8521 tccaccagct tcatcagcaa cggcgtggtc atcatggaaa gcgtcaccgt caccagcaac
     8581 agcgccatct ggtcgccctg gaataaacgt tgtgaagaag cggtagaaaa gaggacaaag
     8641 gcaaactcac cgccctgact caacacgcca gcaaactgca tccgctctga gctacgcacg
     8701 ccatacaatc gcgccagcag atacagcacg agaattttca ccgccaccag cacaaccacg
     8761 cttatcacta cccacaacag atgggtataa agcaccccga ggttgagcga catgccgaca
     8821 gagataaaga acaaaccgag cagcaagcct ttgaagggat cgatagccgt ttccagttca
     8881 tggcgatatt cactttccgc cagcagcacg cccgcaataa atgtaccgag cgccatcgac
     8941 agccccagcg catccataaa caatgcggaa cccaacacca gcagcagcgt cgcggcggtg
     9001 aacacttccc gcacgccaga agctgcaata aagcggaata ccggacgcag taaatagcgc
     9061 ccaccaatca gcatgccgac aaacgccagc accttcatgc cgatcttcat ccagtcgaaa
     9121 tgttcgtctg ccgaccccgc caacaacggc actaacgcca gtgctgggat cactgccaga
     9181 tcctgaaaca gcagaaccga aaacccgagc tggccggatt cgctgcgatt catccctttc
     9241 tcacgcatca attgcaacgc cattgcagtt gaagacatcg caaggccaat gccaccgacc
     9301 accgccgcct gccaggcgaa atccgtcagc atcaataatc ccgccagcaa cgccgcgctt
     9361 aacagcacct gtgccacgcc tacgccaaaa atcgaacgcc gcagttgcca aagtttggag
     9421 ggattcaact caaggccgat gataaacatc aggaatacca cgccgagttc cgaaaagtgg
     9481 aggatctcat cgacgtcgct aataaacccc agcccccacg ggccaattgc aatccctgcc
     9541 agcaaatatc ccaacacagc gccaatcccc agccgcgaag agaaacagca ctcctgcgag
     9601 taaaaaatcg gaaccttcca tcagcggcct cctggagaca gcggatttgc caaccagtca
     9661 ccgtaggctc tggcgtggct cgccagctcc tttgcgcttt gccgtctcgc ccagtaaata
     9721 atgatggaac ttaaccaatg catccggcac atgcccgccg ccagttcaaa ggggcgcagc
     9781 acatcgctca tcgggtagcg attcagcgcg tcataacggt aagcactttc cggctcgccg
     9841 gtggtaatca cgttacgcca gtactttccc gccagttggt ttcctcccgg cccgctggca
     9901 aaaccacgac ttaatacccg gtccagccac tctttcagta gcgccgggca gctataggta
     9961 taaagaggat gctgaaagac aatcacctcg tgctcgcgca gtaatgcctg ctcacggggg
    10021 atatcaataa aaaaatcggg atagtgcgcg taaaggtcgt gcacggtaac attgctgagc
    10081 tgcgtggccg gtttaagcag tacccggttt gccaccgagt cctgagattc cggatgggca
    10141 tacagcagca aaactttcgc tggctgagac atcatccccc tcccggtatg gtttttgtgt
    10201 atagtcgctg ttttgggcta ccattgcgcc cggtgcggca gctcgcccat acattacatt
    10261 atcataatga taagttaaca tagtctgaac atacggcgcc ttatgattgt tttctcctcg
    10321 ttacaaattc gtcgcggcgt gcgcgtcctg ctggataatg ccaccgccac catcaacccc
    10381 gggcagaaag tcggcctggt gggtaaaaac ggctgtggta aatctaccct gctggcattg
    10441 ctgaaaaatg aaatcagcgc cgacggcggc agctacacct ttccgggaag ctggcaactg
    10501 gcgtgggtga atcaggaaac gccagcgtta ccgcaagcgg cgctggaata tgtcattgac
    10561 ggcgaccgtg aatatcgtca actggaagcg cagctgcacg acgccaacga acgtaacgac
    10621 gggcacgcca ttgcgaccat tcatagcaag ctggatgcta ttgacgcatg gagtattcgc
    10681 tcccgtgccg ccagcctgct gcacggcctc ggtttcagca atgaacaact ggagcgcccg
    10741 gtaagtgatt tctccggtgg ctggcgtatg cgtcttaacc ttgcccaggc gctgatttgc
    10801 cgttcagact tgctgctgct cgacgaaccg actaaccacc tcgatctcga tgccgttatc
    10861 tggctggaaa aatggttgaa gagctatcag ggcacgctga tcctgatctc tcacgaccgc
    10921 gacttcctcg atccgatcgt tgataaaatt attcagatcg aacaacaaag catgttcgag
    10981 tacaccggca actacagttc gtttgaagta cagcgcgcca cccgtctggc gcagcaacaa
    11041 gcgatgtacg aaagccagca ggaacgcgtg gcacatctgc aaagttatat cgatcgtttc
    11101 cgtgccaaag ccaccaaagc gaagcaggcc cagagccgca ttaagatgct ggaacgtatg
    11161 gagctgattg cccccgcaca cgtcgacaac ccgttccgct ttagcttccg cgcgccggaa
    11221 agcctgccaa atccgttact gaagatggaa aaggtcagcg caggctatgg cgatcgcatt
    11281 attctcgact cgattaaatt gaatctggtc cccggctcgc gcattggtct gttaggccgc
    11341 aacggcgcag gtaaatcgac attaatcaaa ctgttagccg gtgaacttgc gccagtcagc
    11401 ggtgaaattg gtctggcgaa agggatcaag ctcggctact tcgcccagca tcaacttgaa
    11461 tacctgcgcg ccgacgaatc acctattcaa catctggcac gtttagcgcc gcaggagctg
    11521 gaacaaaaac tgcgtgacta cctcggcggc tttggtttcc tgggcgataa agtaaccgaa
    11581 gaaacgcgcc gcttctccgg tggggaaaag gcccgcctgg tgctggcatt aattgtctgg
    11641 cagcgtccga atctgctgct gctcgacgaa ccgactaacc accttgacct cgacatgcgt
    11701 caggcagtca ccgaagcatt aatcgagttt gaaggcgcgc tggttgtcgt ttcgcacgac
    11761 cgtcatttgc tgcgttccac cactgacgat ctctacctgg ttcacgatcg taaagtcgaa
    11821 ccgttcgacg gcgatctgga agattatcaa cagtggttga gcgacgtaca aaagcaggaa
    11881 aaccagaccg acgaagcgcc aaaagagaac gcgaacaacg cccaggcacg taaagatcag
    11941 aagcgtcggg aagcggagct gcgtgcgcaa acccagccgc tgcgtaaaga gattgcccgt
    12001 ctggaaaaag agatggagaa gctgaacgcg caactggcgc aggcggaaga gaaactcggc
    12061 gacagcgaac tgtatgacca gagccgtaaa gcggagttga ccgcctgcct gcaacagcaa
    12121 gccagcgcca aatccggcct ggaagagtgc gaaatggcgt ggctggaagc ccaggagcag
    12181 cttgagcaga tgctgctgga aggccaaagc aactgatggc gcagataacg acgaccgatg
    12241 ccaatgaatt tagcagcagt gctgaattca cccctatgcg cggctttagc aattgtcatc
    12301 tacaaactat gctgccgcgt ttgtttcgtc gtcaggtgaa attcaccccg tactggcagc
    12361 ggctggagtt gcccgacggc gattttgtcg atctcgcgtg gagtgaagac cccgcacagg
    12421 cgaaacataa accgcgttta gtggtgtttc acgggctgga aggcagtctc aatagccctt
    12481 acgcccacgg tctggttgag gcggcgcaaa agcgcggctg gctgggcgtg gtgatgcatt
    12541 ttcgcggatg cagcggtgaa ccaaaccgta tgcaccgcat ttaccattcg ggcgaaaccg
    12601 aagacgccag ttggttttta cgctggctgc aacgcgaatt tggtcatgcg ccaacggctg
    12661 ccgtcggcta ttcgctcggc ggtaatatgc tggcctgttt gctggcaaag gaaggcaatg
    12721 atctcccggt tgatgcggcg gtgattgtct ctgcgccgtt tatgctggaa gcctgtagct
    12781 atcatatgga aaagggcttt tcccgcgttt atcagcgtta cttgctgaac ctgttaaaag
    12841 ccaatgccgc gcgcaagctg gcagcctacc ccggaacgct gccgattaat ctcgcgcagt
    12901 taaaatcggt acgtcgcatc cgtgaatttg acgatctaat caccgccaga attcacggct
    12961 acgccgacgc tatcgactat tatcgtcagt gtagcgccat gccgatgctg aaccggatcg
    13021 ccaaaccgac gctgattatt cacgccaaag acgatccgtt tatggatcat caggtgatcc
    13081 caaaaccgga aagtctcccc tcgcaggtgg agtatctact gactgaacat ggcggtcatg
    13141 ttggctttat tggcggtaca ttacttcatc cgcaaatgtg gctggagtca cgcattcctg
    13201 actggttaac aacgtatctg gaggcgaaat catgttgatt ccgtggcaag acctctcccc
    13261 cgaaacgctg gaaaatttaa ttgaaagctt tgtgttacgt gaaggcaccg attatggtga
    13321 acatgagcgt acacttgaac agaaagtcgc cgacgtcaaa cgccagctac agtgcggaga
    13381 agcggtgctg gtatggtcgg aactgcacga aacggtcaat attatgccgc gcagtcagtt
    13441 tcgcgaataa cctgcaaccg tcggcggacg agataatagt gatagctaag tcatggagtt
    13501 actatgtctg ccaaacatcc ggtcattgcg gtaacaggat ccagcggcgc ggggaccacc
    13561 accaccagcc tcgcgtttcg taaaatattc gcgcagttaa atctgcatgc agctgaggtg
    13621 gaaggcgaca gttttcaccg ttacacccgc ccggaaatgg acatggcgat ccgcaaagcg
    13681 cgcgacgccg ggcggcatat cagctacttc ggccccgaag cgaacgactt cggcctgctg
    13741 gaacaaacct tcattgaata cggtcagagc ggcaaaggga aatctcgcaa atatctgcat
    13801 acctacgacg aagccgtacc ctggaatcag gtaccgggaa cattcacccc ctggcaacct
    13861 ttaccagaac ccactgatgt actgttttat gaaggtttac acggcggcgt agtcacgcca
    13921 cagcataacg ttgcgcagca tgtggactta ctggtcggcg tagtgcctat cgttaacctt
    13981 gagtggattc aaaaactgat ccgcgacacc agcgagcgcg ggcactcacg agaagcagtg
    14041 atggactcgg tagtgcgttc aatggaagac tatatcaact acatcacacc gcagttttcc
    14101 cgcacccatc ttaacttcca gcgcgttccc accgtcgaca cttcaaaccc gttcgcggca
    14161 aaaggtatcc cgtcgctgga tgaaagcttt gtggtgatcc attttcgtaa tctggaaggg
    14221 atcgatttcc cttggctgct ggcgatgttg caaggctcat tcatttccca catcaatacg
    14281 ttagtggtac ctggcggcaa aatggggctg gcaatggaat taattatgct gctgctggtg
    14341 caacgattga tggaaggaaa gaaaatcgag taactttgct attatgccgg ataaaatact
    14401 atccggcttc acaacgggat agttaagtca cgcggcaacc acttcatacg agtgagtaat
    14461 attcaccgct ttttccagca tcaacgccac tgaacaatat ttctcggcag agagatcaac
    14521 cgcacgcgca accgctgcgt ctttcaggtc gcgaccggtg acgataaaat gcagattaat
    14581 gtgcgtaaac aggcgtggtg cctcttcgcg gcgttcagag gtcaatttta cttcacaatc
    14641 gaccacatcc tgacgccctt tttgcaggat cgaaaccaca tcgatggcac tgcaaccacc
    14701 cgccgccatc aacaccattt ccatcggact tggtgcttta tcgcctgagt tgccgtccat
    14761 taaaatctgg tgaccagagg cggattcgcc cagaaaagtt aacccttcga cccacttcac
    14821 tcgcgcttgc atttttgcta ctccactgcg tcaattttcc tgacagagta cgcgtactaa
    14881 ccaaatcgcg caacggaagg cgacctaggt catgctgaag cgagacacca ggagacacaa
    14941 agcgaaagct atgctaaaac agtcaggatg ctacagtaat acattgatgt actgcatgta
    15001 tgcaaaggac gtcacattac cgtgcagtac agttgatagc cccttcccag gtagcgggaa
    15061 gcatatttcg gcaatccaga gacagcggcg ttatctggct ctggagaaag cttataacag
    15121 aggataaccg cgcatggtgc ttggcaaacc gcaaacagac ccgactctcg aatggttctt
    15181 gtctcattgc cacattcata agtacccatc caagagcacg cttattcacc agggtgaaaa
    15241 agcggaaacg ctgtactaca tcgttaaagg ctctgtggca gtgctgatca aagacgaaga
    15301 gggtaaagaa atgatcctct cctatctgaa tcagggtgat tttattggcg aactgggcct
    15361 gtttgaagag ggccaggaac gtagcgcatg ggtacgtgcg aaaaccgcct gtgaagtggc
    15421 tgaaatttcg tacaaaaaat ttcgccaatt gattcaggta aacccggaca ttctgatgcg
    15481 tctgtctgca cagatggcgc gtcgtctgca agtcacttca gagaaagtgg gcaacctggc
    15541 gttcctcgac gtgacgggcc gcattgcaca gactctgctg aacctggcaa aacaaccaga
    15601 tgctatgact cacccggacg gtatgcaaat caaaattacc cgtcaggaaa tcggtcagat
    15661 tgtcggctgt tctcgtgaaa ccgtgggacg cattctgaag atgctggaag atcagaacct
    15721 gatctccgca cacggtaaaa ccatcgtcgt ttacggcact cgttaatccc gtcggagtgg
    15781 cgcgttacct ggtagcgcgc cattttgttt cccccgatgt ggcgcagact gatttatcac
    15841 cccgatatca actatgcact tcgacaaacg ctggtgctat gtttgcccgt ggccgttggg
    15901 ttaatgcttg gcgaattacg attcggtctg ctcttctccc tcgttcctgc ctgttgcaat
    15961 attgcgggcc ttgatacgcc tcataaacgt tttttcaaac acttaatcat tggtgcgtcg
    16021 ctgtttgcca cctgtagctt actgacacag ctgctactgg taaaagatgt tcccctgccc
    16081 tttttgctga ccggattaac gctggtactt ggcgtcactg ctgagctggg gccattgcac
    16141 gcaaaattgc ttcctgcatc gctgctcgcc gccattttta ccctcagtct ggcgggatac
    16201 atgccggtct gggaaccatt gctcatctat gcgttgggca ctctctggta cggattgttt
    16261 aactggtttt ggttctggat ctggcgcgaa caaccgctgc gcgagtcact aagtctgctg
    16321 taccgtgaac tggcagatta ttgtgaagcc aaatacagcc tgcttaccca gcacaccgat
    16381 cctgaaaaag cgctgccacc gctgctggtg cgccagcaaa aagcggtcga tttaattact
    16441 cagtgctatc agcaaatgca tatgctttcc gcgcaaaaca ataccgatta caagcggatg
    16501 ctgcgtattt tccaggaggc gctggatttg caggaacata tttcggtcag tttgcatcag
    16561 ccggaagagg tgcaaaaact ggtcgagcgt agccatgcgg aagaagttat ccgctggaat
    16621 gcgcaaaccg tcgccgctcg cctgcgcgtg ctggctgatg acattcttta ccatcgcctg
    16681 ccaacgcgtt ttacgatgga aaagcaaatt ggcgcactgg aaaaaatcgc ccgccagcat
    16741 ccggataatc cggttgggca attctgctac tggcatttca gccgcatcgc ccgcgtgctg
    16801 cgcacccaaa aaccgctcta tgcccgtgac ttactggccg ataaacagcg gcgaatgccg
    16861 ttgcttccgg cactgaaaag ttatctgtca ctaaagtctc cggcgctacg caatgccgga
    16921 cgactcagtg tgatgttaag cgttgccagc ctgatgggca ccgtgctgca tctgccgaag
    16981 tcgtactgga tcctgatgac ggtattgctg gtgacacaaa atggctatgg cgcaacccgt
    17041 ctgaggattg tgaatcgctc cgtgggaacc gtggtcgggt taatcattgc gggcgtagcg
    17101 ctgcacttta aaattcccga aggttacacc ctgacgttga tgctgattac caccctcgcc
    17161 agctacctga tattgcgcaa aaactacggc tgggcaacgg tcggttttac tattaccgca
    17221 gtgtataccc tgcaactgtt gtggctgaac ggtgagcagt acatccttcc acgtcttatc
    17281 gataccatta ttggttgttt gattgctttc ggtggtaccg tctggctgtg gccgcagtgg
    17341 cagagcgggt tattgcgtaa aaacgcccat gatgctttag aagcctatca ggaagcgatt
    17401 cgcttgattc tgagcgagga cccgcaacca acgccactgg cctggcagag aatgcgggta
    17461 aatcaggcgc ataacactct gtataactca ttgaatcagg cgatgcagga accggcattc
    17521 aacagccatt atctggcaga tatgaaactg tgggtaacgc acagccagtt tattgttgag
    17581 catattaatg ccatgaccac gctggcgcgg gaacaccggg cattgccacc tgaactggca
    17641 caagagtatt tacagtcttg tgaaatcgcc attcagcgtt gtcagcagcg actggagtat
    17701 gacgaaccgg gtagttctgg cgatgccaat atcatggatg cgccggagat gcagccgcac
    17761 gaaggcgcgg caggtacgct ggagcagcat ttacagcggg ttattggcca tctgaacacc
    17821 atgcacacca tttcgtcgat ggcatggcgt cagcgaccgc atcacgggat ttggctgagt
    17881 cgcaagttgc gggattcgaa ggcgtaatac aggctaaacc atcggataag gcattcacgc
    17941 cgcatccgac atattttgcc tgatgcgtct tatttacgcc ccaaccacct tcgccaccgc
    18001 gtgggcgaaa cgttgcatcc cctcatcgat atccgcatct tccaccacca gcgacggtgc
    18061 taaacgcatc acatccggtc cggcattcag caccattacg ccaacctctg cacccgcata
    18121 caggaaatca cgcgcccgac ctttgtagtg tggtttcagc tccgcgccaa ttaacagccc
    18181 cataccgcga atatcgctaa acacatcgta ctgctgatcg atcttctgca aatggtcaac
    18241 aaaacgctgg cgtttcgcct gaatgccttc cagcacttca ggggtattga tgatatcaaa
    18301 cgccgcgccc gctactgcac aggccagagg attaccgccg taggtggaac cgtgagaacc
    18361 aggatgaaac gcagaagcaa tttccgccgt ggtcagcatg gcgctaatcg ggaagccgcc
    18421 gcctaacgct ttcgcagaag tcagaatatc cggcgtcaca ccgtagtacc tgtaagcaaa
    18481 caaatcgcca gtccgcccca tcccgcactg cacttcatca aacaccaata atacctgatg
    18541 ttgatcgcac agctcgcgca agccctgcaa aaactctggc gtcgctgccg tcacaccgcc
    18601 ctcgccctgg atcggctcaa ccaccaccgc acaggtgtga tcatccatca ccgctttcac
    18661 tgcatggaga tcgttaaagg gaacgtggat gatgtctgcc ggttgcggcc caaagccgtc
    18721 ggaatatttt ggctgcccac ccaccgaaac ggtaaacagc gagcgaccat gaaaagcgtt
    18781 atggaaggca ataattttgg ttttgaacgg gctatgacgc acacaggcgt aatggcgtgc
    18841 cagtttaaag gcggtttcgt tagcttccgt gccggagttc ataaacacca cgcgttcggc
    18901 aaacgtcgcc tcaatcagtt tgcgcccaag acgcagcgcc ggttcattgg tgaaaacgtt
    18961 actgatatgc cacagagttt cgccctgggt ttttaacgcg ttcaccagcg caggatggca
    19021 atggcccaac gccgtaactg caatgccacc cgcgaaatcg acatactcct tgccttgctg
    19081 atcccagatt cggctgccct gaccttttac cggaataaac tctgccggag cataaatcgg
    19141 caggatcact tcatcgaaag tcgcgcgtgt aattgctgtt tgttcaattg ccatctcatg
    19201 atcaccctgt tacgcataaa taaatatgaa attataatca caaaatatgc ataaaaaatc
    19261 actgaatggc aatcggaaat cagagatgca ggaaattagc cagcagttga tgtccttgtt
    19321 cgctaagaat actttctgga tggaactgca caccttccag atcccactgg cgatggcgaa
    19381 tccccataat ctcgcgggtt tcgctccagg ccgtcacgtc aaagcacgct ggtaatgagt
    19441 caggttccac caccagcgaa tggtagcgtg tcacggtaag tggatttgcc agcccccgaa
    19501 atacgccctc accgctatgt gtaatcggcg aggttttgcc gtgcatgacc tttgcggcgc
    19561 gcacaacttt gccgccaaat gcctgcgcca ttgcctgatg accgaggcag acgccaagaa
    19621 tcggcaagcg cccggcatag tggcgaataa cgtcaaggga gatcccggct tcatctggcg
    19681 tacaggggcc aggtgagatg acaatttttt gtggtttaag ggcgtcgata tccgccagcg
    19741 tcaacgcatc gttgcgctta accagcacat ccgcccccag ttcacaaaag tactggtaga
    19801 ggttccaggt aaaagaatcg tagttatcta taagcaggat catggcggct ccggtacgaa
    19861 agaaccgcgc tattttactc agattccccg gcttcgctta ccactttgct aaatatcatc
    19921 tgcagtgcgg tgagatcccc cattgcccca ctctgatttg cctgattcca ggcctctttt
    19981 tcgataccct gccagctcag ttgatatccg gcatgaatcg ccagttgctc gaagaagatc
    20041 cgctgtgcca gaccacttcc cacccggaag ggatgcagca cgtcgatttc acaatagtaa
    20101 tgcgccagcc gctcgacgaa cttcgctttc tccaggccaa ccagataacc ttcttcctcc
    20161 agatcctgca tcagggcatt gccctctttt tcgatataag caaagtggca gaacggcgta
    20221 tcgccctgat aaatatcaac ttcacgcagt tgccctgccc agtcgaaaat atcctggtac
    20281 agctggcgat ggatagtgcg caaatgcggt aaaccgcgca ccagcggacc aagctcaatg
    20341 gtcgcagcac gcagcgccgt catttcgtaa gcggcctgtt ccagccgctg ctgctggcgg
    20401 atgttcagcc ggttacgcat gatatcaagg ccaggataaa gatacggatc gcgcccttcg
    20461 ccgaatttat cgctcatagt gcctcctcag ctcttcaagg cgcgctaaag cctctgcggc
    20521 agtaagagtg actaaaggca tctcgacgcc ttcaagacgg cgactggcct ggaaattacg
    20581 attacgctga agctcccaga gacgggactt ttgcttatcg gtgagtttct tcacttgatg
    20641 cctccctgaa cgtgtccatt tgccacaagt ataagcggca aatccgggtt acgccgggag
    20701 agcagaagcg cgggcaagat tgcccgcgcg agaaatcatt acggcaggac tttagcggaa
    20761 aggataacta ccggttttga cggcacattc tggtacggac caacatcatg agtcggcacc
    20821 tgggaaatct tatcggcaac gtccatgcct ttcaccactt taccaaatac cgcgtaaccg
    20881 aaatcacgct gaccatggtc aaggaaggcg ttatcggcaa cgttgataaa gaactggctg
    20941 gtggcgctgt ctttgtcagc ggtacgcgcc atcgcgatgg tgccacgcgt gttgcgcagg
    21001 ccgttatcgg cttcattttt gattggcggg tttggttttt tctgctgcat ctgctcggtg
    21061 aaaccgccgc cctgaatcat aaagccagga atgacgcggt gaaaggtagt gttgttataa
    21121 aaaccgctgt tcacataatc gacaaagttt tgcacagaca ctggcgcttt ttgtttatcc
    21181 agctccagtt cgatgttacc agctgaggtt gtcaacaata cgtgcgggtc ccctttcgct
    21241 gccattgctg cgggagaaag agcagaaaga gcgaaaacag cagccatcgc cgccagggtc
    21301 gatttgaaca tgagatttcc ttaacaaaca gagcagaaaa aaagcaagtg aagagattct
    21361 aaatagcctt caagaacaag gccatccctt tacctaattt tacgtatctg aaatatatgt
    21421 aacctgacga tcacttttta ttccgtgatc aaaatcacct cttaaaatgc aatttagcaa
    21481 ccgattgcaa taaaacattt aaacagatca caaaatcacc taaaatcgcc cgctcgcggc
    21541 atatcccgct atgccatttt atttttgaac gcaggccaat catgactaac agcaatcgca
    21601 tcaagctcac atggattagc tttctctcct acgcactgac tggtgcgttg gttattgtca
    21661 ccgggatggt gatgggaaat atcgccgatt atttcaatct gcctgtttcc agtatgagta
    21721 acaccttcac cttcctcaac gccggcattt taatctctat cttcctcaac gcctggctga
    21781 tggaaatcgt cccgttgaaa acgcagttac gttttggctt tctcctgatg gtgctggcgg
    21841 ttgccggttt gatgttcagc cacagcctgg cgctgttctc gacggcgatg ttcattctcg
    21901 gggtggtcag cggcattact atgtcgattg gtacattcct gataacacaa atgtatgaag
    21961 ggcgtcagcg cggttcccgc ctgttattta ctgactcctt cttcagtatg gctgggatga
    22021 ttttcccaat gatcgccgcg tttctactgg cgcgcagcat tgagtggtac tgggtttatg
    22081 cctgcatcgg gctggtgtat gtcgctattt ttattctgac cttcagctgt gagttcccgg
    22141 cactgggcaa acgtgcacca aaaacggatg ctccggtaga gaaagaaaag tgggggatcg
    22201 gcgtactgtt tctctctgtt gcggcgctgt gctacatcct cggtcagtta ggttttatct
    22261 cctgggtgcc tgagtatgcc aaaggcctgg gcatgagcct gaacgacgcg ggcacgctgg
    22321 tgagtaactt ctggatgtca tacatggtcg gcatgtgggc gttcagcttt attcttcgct
    22381 tctttgattt gcaacgcatt ctgaccgtac tggctggtct ggctgcgatt ctgatgtacg
    22441 tctttaacac cggaacacca gcacatatgg cgtggtcaat tctcgctctg ggcttcttct
    22501 ccagcgcgat ctataccacc atcattaccc tgggttcaca gcagaccaaa gtaccgtcgc
    22561 caaaactggt taactttgtc ctgacctgcg gaaccatcgg tactatgttg acctttgtgg
    22621 ttaccggccc gattgttgaa catagcggtc cgcaggcggc actgcttaca gcaaacggtc
    22681 tgtacgctgt cgtctttgtg atgtgcttcc tgttaggttt cgtcagccgt caccgtcagc
    22741 ataacaccct gacctctcat taattgctaa tgccggacgg cactatcgtc gtccggcctt
    22801 ttcctctctt cccccgctac gtgcatctat ttctataaac ccgttcattt tgtccatttt
    22861 ttgcacaaac atgaaatgtc agacaattcc gtgacttaag aaaatttata caaatcagca
    22921 acatacccct taaggagtat ataaaggtga atttgattta catcaataag cggggttgct
    22981 gaatcgttaa ggtaggcggt aatagaaaag aaatcgaggc aaaaatgagc aaagtcagac
    23041 tcgcaattat cggtaacggt atggtcggcc atcgctttat cgaagatctt cttgataaat
    23101 ctgatgcggc caactttgat attaccgttt tctgtgaaga accgcgcatc gcttatgacc
    23161 gcgtacacct ctcgtcttac ttctctcacc acaccgccga agagctgtcg ctggtgcgcg
    23221 aaggcttcta cgagaaacac ggcatcaaag ttctggtcgg cgaacgcgct atcaccatca
    23281 accgtcagga gaaggtgatt cactccagcg ccggacgtac cgttttttat gacaagctga
    23341 tcatggcaac cggttcctac ccgtggatcc caccaatcaa aggttctgat actcaggact
    23401 gctttgtcta tcgcaccatt gaagacctca acgccattga atcctgcgcc cgtcgcagca
    23461 aacgcggtgc cgttgttggt ggcggcctgt taggtctgga agccgctggc gcgctgaaaa
    23521 acttaggtat tgaaacccac gttatcgaat ttgcccctat gctgatggcg gaacagcttg
    23581 atcagatggg tggcgagcag ctgcgtcgca aaatcgaaag tatgggcgtg cgcgttcact
    23641 ccagcaaaaa cacccttgag attgtgcagg aaggtgtcga agcgcgtaaa accatgcgtt
    23701 ttgccgacgg cagcgaactg gaagtcgact ttatcgtctt ctctaccggt atccgtccgc
    23761 gcgataagct ggcaacccag tgtggtctgg acgttgctcc gcgtgggggg attgtcatta
    23821 atgattcctg ccagacttcc gatccggata tctacgccat cggtgaatgc gcaagctgga
    23881 acaaccgtgt atttggtctg gtagcaccag gctacaaaat ggcgcaggtc gccgttgacc
    23941 atattctggg tagcgaaaac gcctttgaag gtgctgacct tagcgccaag ctgaaactgc
    24001 tgggcgtaga cgtaggtggt attggtgatg cgcacggtcg cacgcctggc gcacgtagct
    24061 atgtttacct cgacgaaagt aaagagatct acaaacgcct gattgtcagc gaagacaaca
    24121 aaaccctgct cggtgcggta ctggtgggcg ataccagcga ctacggcaac ctgctgcaac
    24181 tggtgctgaa cgctatcgag ctgccggaaa acccggattc tctgattctg ccggcgcact
    24241 cgggtagcgg taagccgtct atcggtgttg ataaactgcc ggacagcgcg caaatctgct
    24301 cctgcttcga cgtcactaaa ggtgatctga ttgctgccat caacaaaggc tgccacacag
    24361 ttgcggcgct gaaagctgaa accaaagcgg gtactggctg cggtggctgt atcccgctgg
    24421 tcactcaggt actgaacgcg gaactggcga aacagggcat cgaagttaac aacaacctgt
    24481 gcgaacactt tgcttattcg cgtcaggaac tgttccattt gatccgcgtt gaaggcatta
    24541 aaaccttcga agaactgctg gcgaaacacg gcaaaggcta cggttgtgaa gtttgtaaac
    24601 caaccgtcgg ttcgctgctg gcctcctgct ggaacgaata cattctgaag ccggaacata
    24661 ctccgctgca ggattctaac gacaacttcc tcgctaacat ccagaaagac ggcacctact
    24721 cggtgatccc gcgttctccg ggcggtgaaa tcaccccgga agggctgatg gcagtgggtc
    24781 gtatcgcgcg taaatttaat ctctacacca aaatcaccgg ctcccagcgc ctggcgatgt
    24841 ttggcgcaca gaaagacgat ctgccggaaa tctggcgtca gctgattgaa gctggcttcg
    24901 aaaccggtca tgcctatgcg aaagcactgc gtatggcgaa aacctgcgtg ggtagcacct
    24961 ggtgccgcta cggtgttggc gatagcgtcg gcctcggcgt ggaactggaa aaccgctaca
    25021 aaggcatccg tacgccgcac aaaatgaagt tcggtgtctc cggctgtacc cgtgaatgtt
    25081 cagaagctca gggtaaagac gtgggtatta tcgccactga aaaaggctgg aacctgtatg
    25141 tttgcggtaa cggcggcatg aaaccgcgtc atgcggatct gctggcagcg gatatcgatc
    25201 gcgaaacgct gatcaaatat ctcgaccgct tcatgatgtt ctacatccgt accgccgaca
    25261 aactgacgcg taccgcgccg tggttagaaa acctcgaagg cggcatcgat tacctgaaag
    25321 cggtgatcat tgacgacaaa ctggggctga acgctcatct ggaagaagag atggcgcgcc
    25381 tgcgtgaagc ggtagtgtgt gagtggactg aaacggtcaa tacaccgtcc gcgcagactc
    25441 gcttcaaaca cttcatcaac agcgacaagc gtgacccgaa cgtgcagatg gtgccagagc
    25501 gcgaacagca ccgtccggca acgccgtatg aacgtatccc ggtaactctg gtggaggaca
    25561 acgcatgagc cagtggaaag acatctgcaa aatcgatgac atcctgcctg aaaccggcgt
    25621 ctgcgcgctg ttaggtgacg agcaggtagc gattttccgc ccgtatcaca gcgaccaggt
    25681 gtttgcgatc agcaacattg atccgttctt cgagtccagc gtgctatcac gcgggctgat
    25741 tgcggaacac cagggcgagc tgtgggtcgc cagcccgctg aaaaaacagc gttttcgctt
    25801 aagtgatggc ttgtgcatgg aagacgaaca gttttccgtc aaacattacg aagcacgagt
    25861 gaaagacggc gtggtgcagc tgcgcggtta atgttttaac gggaggcgca atgcctcccc
    25921 tttttgcatg gtcctgtaat gaccttcggt atattgcagg acatttttta aactttttgt
    25981 tttatttttt gtttttattt tttaaaggat aatcaaatgt ttacagacac tattaataag
    26041 tgtgcggcta atgctgcgcg cattgcacgc ctgtcggcaa ataacccgct cggcttttgg
    26101 gtcagctccg ccatggcggg cgcatatgtg ggtcttggga tcatcctgat tttcacgctc
    26161 ggtaatttgc tcgacccgtc cgtacgtccg ctggtgatgg gcgcgacctt tggtatcgcc
    26221 ttaacgctgg tgattatcgc cggttctgaa ctgttcaccg gacacaccat gttcctcacc
    26281 tttggggtaa aagcgggcag catcagccac gggcaaatgt gggcaatcct gccgcaaacc
    26341 tggctgggta acctggtcgg ttccgtcttc gttgccatgc tctatagctg gggcggcggt
    26401 agcctgctgc cggtagatac cagcatcgtt cactccgtcg cgctggctaa aaccactgca
    26461 ccggcaatgg tactgttctt caaaggtgca ttgtgtaact ggctggtttg cctggcaatc
    26521 tggatggcgc tgcgcactga aggggcggcg aaatttatcg ctatctggtg gtgtctgctg
    26581 gcatttatcg cgtccggcta cgagcactct atcgctaaca tgacgctgtt cgcgctctcc
    26641 tggttcggca accacagcga agcctacacg ctggcgggta ttggtcataa cctgctgtgg
    26701 gtgacgctgg gtaatacttt atcaggtgcc gtattcatgg gattgggtta ttggtatgct
    26761 acgccgaaag cgaatcgtcc ggttgcggac aaatttaatc aaactgaaac ggctgccggt
    26821 taattactaa ggggttttta cgtggatcat ttgcctatat tttgccaatt acgcgatcgc
    26881 gactgtctga ttgtcggcgg tggtgatgtc gcggaacgca aagcaaggtt gctgttagac
    26941 gcaggcgctc gcttaacggt gaatgcatta gcgtttattc cacagttcac cgcatgggca
    27001 gatgcaggca tgttaaccct cgtcgaaggg ccatttgatg aaagccttct cgacacctgc
    27061 tggctggcga ttgcagcgac ggatgatgac acgcttaacc agcgcgtcag cgaagccgct
    27121 gaagctcgtc gcatcttctg taacgtagtc gatgcgccga aagccgccag ctttattatg
    27181 ccgtcgatta ttgaccgctc accgctaatg gtagcggtct cctctggcgg cacctctccg
    27241 gttctggctc gcctgttgcg cgaaaaactc gaatcactgc tgccgttgca tctgggccag
    27301 gtggcgaaat atgccgggca attacgcggg cgagtgaaac aacagttcgc cacgatgagt
    27361 gagcgtcgcc gtttctggga gaaattgttc gttaacgacc gcctggcgca gtcgctggca
    27421 aacaacgatc agaaagccat tactgaaacg accgaacagt taatcaacga accgctcgac
    27481 catcgcggtg aagtggtgct ggttggtgca ggtccgggcg atgccgggct gctgacgctg
    27541 aaaggactgc aacaaattca gcaggcagat gtggtggtct acgaccgtct ggtttctgac
    27601 gatattatga atctggtacg ccgcgatgct gatcgcgttt tcgtcggcaa acgcgcggga
    27661 taccactgtg taccgcagga agagattaac cagatcctgc tgcgggaagc gcaaaaaggc
    27721 aaacgcgtgg tgcggctgaa aggtggcgat ccgtttattt ttggccgtgg tggcgaagag
    27781 ctggaaacac tgtgcaacgc gggtattccg ttctcggtgg ttccgggtat taccgcagct
    27841 tctggttgct ctgcctattc gggtattcca ctcacgcatc gcgattatgc ccagagcgta
    27901 cgcttaatta ccggacactt aaaaaccggt ggcgagctgg actgggaaaa cctggcggca
    27961 gaaaaacaga cgctggtgtt ctatatgggg ttgaatcagg ccgcgactat tcagcaaaag
    28021 ctgattgaac acggtatgcc tggcgaaatg ccggtggcaa ttgtcgaaaa cggaacggca
    28081 gtcacgcagc gcgtgattga cggtacgctc acgcaactcg gtgaacttgc tcagcaaatg
    28141 aacagtccat cgctaattat tattggtcgg gttgttggcc tgcgcgataa actgaactgg
    28201 ttctccaatc attaatttaa cccgggccag agaattctgg ccttcttaat gactcttttt
    28261 atatattcag caaataaaac ataacccctt aataaatatt atctgctaat gggttatatt
    28321 caccgttaca gcgacttaca aaattaagtc attcgttcac tgacgccaat taaatattcc
    28381 tgtatcgttc ttaacgagca gtctgcttat attcatataa tcaatgaata ttaattaata
    28441 tataatacat agggaatgta aatgaacaaa tttattaaag ttgcactggt aggtgcagta
    28501 ctggctacgt taactgcatg tactggtcat attgaaaacc gtgataagaa ctgctcttac
    28561 gactacctgc tgcacccggc aatttctatt tctaaaatca ttggcggttg cggtcctact
    28621 gcacagtaag tctctgacag aaatcggcta acaccggttt aattgcccga cgccctctgg
    28681 attttccgag ggcgtatttg tttctatgtc cttcttgccc ccgcccgtaa aaatattttc
    28741 actttaaatt caattttata actacatcca ttagccacag cgcgatcctg tgcgaaattt
    28801 tgtgatcttc ctccacatta cacaacatca tatgttgtta tattcatcat gcattgtcat
    28861 gttgcctttt aaatgactgc aaactctccc ctacaacgta ttggacaaga aaaaggtatc
    28921 gctatgggaa gccaggaact ccaacgcaag ctcggatttt gggccgttct tgcaatcgcc
    28981 gtcgggacaa ccgtcggctc cggtattttt gtatctgtgg gtgaagtggc aaaagcagcg
    29041 ggcacgccgt ggcttacggt gctcgcgttt gtcattggcg ggttaattgt gatcccgcaa
    29101 atgtgcgtct atgcggaact atccaccgct tatccggaaa atggcgcaga ttatgtttat
    29161 ctgaaaaatg ccggaagccg accgctggct ttcctctccg gctgggccag cttctgggcc
    29221 aacgatgcgc cgtcattgtc gattatggcg ctggcgattg tcagcaatct tggcttttta
    29281 acgcctatcg atccgttgct cggtaaattt atcgccgccg gattaattat cgcctttatg
    29341 ttgctacacc tgcgctccgt tgaaggcggc gcagcgtttc agacgctaat taccatcgcc
    29401 aaaattatcc cgttcactat cgtcattggc cttgggatct tctggtttaa agcggagaat
    29461 tttgccgccc ttaccaccac tgcgattggc gcaacgggca gctttatggc gctgctggcg
    29521 gggatctctg ccaccagttg gtcgtatacc ggcatggcct ctatctgtta tatgaccggc
    29581 gaaattaaaa accccggaaa aaccatgcca cgagcgctga ttggttcctg tctgctggtt
    29641 ctggtgctct acaccctgct ggcgctggtg atttccggcc tgatgccctt cgacaaactc
    29701 gccaattctg aaacgccgat ttccgacgcc ctgacctaga tccccgcact cggcagcacc
    29761 gctgggatct ttgttgccat cacggcgatg atcgtcattc ttggttcgct ttccagctgc
    29821 gtgatgtacc agccgcggct ggaatacgcg atggcgaaag acaacctgtt ctttaaatgc
    29881 ttcggccatg tgcatccgaa atacaacacg ccggatgtct ccatcatcct gcaaggggcg
    29941 ctggggatct tcttcatctt cgtttccgat ctcaccagcc tgctgggtta tttcaccctg
    30001 gtgatgtgtt tcaaaaatac cctcaccttc ggctccatca tctggtgtcg taaacgcgac
    30061 gattacaaac cgctgtggcg tactccggct ttcgggctga tgaccaccct cgccattgcg
    30121 tcaagcctca ttctggtcgc ctcaaccttt gtctgggcac cgattcccgg ccttatctgc
    30181 gccgtcatcg ttattgctac tggtctgcct gcttacgcct tctgggcgaa gcgtagccgc
    30241 cagctcaacg ctttgtcgta atgttatctg aagaaaataa aatgttggat attgataaaa
    30301 gcaccgtgga ctttctggtc accgaaaata tggttcagga agtggaaaaa gttctcagcc
    30361 atgacgttcc gctggtgcac gccatcgtgg aagagatggt gaagcgcgac attgatcgta
    30421 tttatttcgt tgcctgcggc tcgccactca acgcggcgca aacggcgaaa catctggcgg
    30481 atcgcttttc cgatcttcag gtctacgcca tttccggctg ggagttctgc gataacaccc
    30541 cgtatcgcct cgacgatcgt tgcgcggtga ttggcgtttc tgactacggt aaaacggaag
    30601 aggtaatcaa agcgctggag ctgggccggg cctgcggcgc actcactgcg gcgttcacca
    30661 aacgcgcgga cagcccgatt acctcggcgg cggaatttag cattgattat caggccgact
    30721 gtatctggga aattcacctg ctgctctgct acagcgtggt gctggagatg atcacccgcc
    30781 tcgcgccgaa cgcggaaatc ggcaagatca aaaacgatct caagcagttg ccgaatgcgc
    30841 tcggtcatct ggtacgcacc tgggaagaaa aaggccgcca gcttggtgaa ctggccagcc
    30901 agtggccgat gatttatacc gttgctgcgg gtccgctgcg tccgctgggt tacaaagaag
    30961 gcattgtaac gctgatggaa tttacctgga cgcacggctg cgtgattgag agcggagagt
    31021 tccgccatgg cccgctggag attgtcgaac cgggcgttcc gttcctgttc ctgctcggca
    31081 atgatgaaag tcgccacacc accgaacgcg ccattaactt tgttaaacag cgtactgaca
    31141 acgtgatcgt catcgattac gccgaaattt cgcaagggct gcacccgtgg ctggcaccgt
    31201 tcctgatgtt cgtgccaatg gagtggctct gctactacct gtctatttac aaagatcaca
    31261 acccggatga acgccgctat tacggtggtc tggtggaata ttaatccctc tcccggcccg
    31321 gcaacgggcc ggatttacgc aaggagttac ccgatgaaaa caggtatgtt tacctgcggc
    31381 caccagcggc tgcctattga acacgcattt cgtgatgcaa gcgagctggg ttacgacggc
    31441 atcgaaattt ggggcggtcg cccgcacgcg ttcgcgccgg acttaaaagc gggcggcatc
    31501 aaacaaatca aggcgctggc gcagacgtat cagatgccga ttatcggtta cacgccagaa
    31561 accaacggct atccgtataa catgatgctg ggcgatgaac atatgcgtcg tgaaagcctg
    31621 gacatgatca agctggcgat atatatggca aaagagatga acgcgggtta tacgctgatt
    31681 tctgcggccc acgcgggcta tctcacgccg cctaacgtta tctggggcgg ctggcagaga
    31741 acttaagcga actgtgtgag tacgcggaaa acatcggcat ggatctgatc ctcgaaccgt
    31801 taacgccgta tgaatcgaac gtcgtgtgta atgctaatga tgtgcttcat gcgctggcgc
    31861 tggtgccttc gccgcgcctg ttcagcatgg tcgacatttg cgcgccgtat gtccaggcgg
    31921 aaccggtgat gagttatttc gacaaactgg gcgataaatt acgtcatctg catattgtcg
    31981 acagcgacgg ggccagcgac acgcattaca ttcctggaga aggcaaaatg ccgctgcggg
    32041 aactgatgcg cgatattatt gatcggggct atgagggtta ctgtacggtg gagctggtga
    32101 cgatgtatat gaacgagccc agactctatg cccgccaggc gctggaacgc tttcgcgcgc
    32161 tgctgccgga ggatgagaga tgaaaaccct agcgacaatc ggcgataact gcgtcgatat
    32221 ctacccgcaa ctgaataaag cgttttctgg cggtaatgcg gtcaatgtgg cggtgtactg
    32281 cactcgctac ggcatacagc cgggatgcat tacctgggtg ggtgacgatg actacggcac
    32341 aaagctgaag caggatctcg cccgcatggg cgtcgatatc agccatgtcc atacgaaaca
    32401 cggcgttacc gcacaaactc aggtggaact gcacgacaat gatcgcgttt ttggcgacta
    32461 caccgaaggc gtgatggccg actttgccct gagtgaagag gattacgcct ggctggcgca
    32521 gtatgacatt gtgcacgcgg caatctgggg acatgcggaa gacgcattcc cacagctgca
    32581 cgctgcgggc aaacttaccg ctttcgactt ctccgacaag tgggacagcc cgctctggca
    32641 gacactggtg ccgcatctcg attttgcctt tgcctccgca ccgcaagaag acgaagcgct
    32701 gcgtctgaag atgaaagcga ttgttgcccg tggcgcagga acagtgattg tcacgctggg
    32761 tgaaaacggc agcattgcct gggatggcgc gcagttctgg cgtcaggctc ctgaaccggt
    32821 gacggttatc gacaccatgg gtgccggaga ttcgttcatt gccggattcc tttgcggctg
    32881 gtctgcgggg atgacattac cgcaggcgat ggcgcaggga acggcgtgcg cggcgaaaac
    32941 cattcagtac cacggtgcct ggtaggtata acgttggcgt gagcatcttc acgccaacgt
    33001 gctgttactt gccggaaaac gaccctataa tccgagtaat tcattcttta tttcagggtc
    33061 gattatgtca gctacggacc gctactctca tcaactcctc tacgctaccg tccgccagcg
    33121 gctgctggat gatatcgcgc agggggttta ccaggccggg caacagatcc ctaccgaaaa
    33181 cgagctttgt acacaatata acgtcagccg cattaccatt cgcaaagcca tcagcgactt
    33241 agtggcagac ggcgtactga tccgctggca gggaaaaggc acctttgtac aaagccagaa
    33301 agttgaaaac gccctgctta ctgtcagtgg ttttaccgat tttggcgtct cacaaggcaa
    33361 ggcgacgaaa gagaaagtga tcgaacagga acgggtcagc gccgcgccgt tttgcgaaaa
    33421 gctgaacatc cccggaaaca gcgaagtgtt ccatctctgc cgggtgatgt atctcgataa
    33481 agagccgctg tttattgata gttcatggat cccgctgtcg cgttatcctg actttgatga
    33541 gatttacgtc gaaggaagct ccacctatca gttatttcag gagcgttttg acacgcgagt
    33601 ggtcagcgac aaaaagacca tcgatatctt tgccgccacc cgcccgcagg caaaatggct
    33661 gaaatgcgaa ctgggcgaac cgttgtttcg catcagcaaa atcgcctttg atcagaatga
    33721 caaaccggtg cacgtctccg aactcttctg ccgcgccaat cgcatcacct taacgattga
    33781 taataaaaga cattaaccgt aggccggata agatgcgcca gcatcgcatc cggcgatgct
    33841 ggcgcactga attttacatc ccgcacgttc ccctcaccct aaccctctcc ccaaaggggc
    33901 gaggggagcg ttcagtgcgg aatttagctt gattgccgaa cggtgctatc cagcattaaa
    33961 tactcgcaat actctcacgc aaaatccgca gcaccgtctc ttcaccgctg cgattcgggt
    34021 taatgcgaat cgcacaatgt tcgagttgtg gattcgcctg gcgaaacgtt cctgaaagtc
    34081 gatagaagag cggcgggatt tcatatttcg actctgcacc gaccgggtaa ggcaacgcgc
    34141 cacgcttttg cgcttcttcc agcaccctgg cggcaatcag ctgatgaaac tcgacaatca
    34201 acaccttcga ctgcgcattg gcaatgacgg cgcttttcac ttccgccacc gcgccgccgt
    34261 taagcaaagc cagcaaccgt tcggatactc ccgcctgcac cgcatgcatc actggcgcga
    34321 acaccagacc acgcaagact tccagcgcct gtgagccctg gatctggcta ccgccggagt
    34381 aaagcgtggc gcgaatccga ctaataacat cggcatcgcc aaccactgca ccaacgccct
    34441 ccggcccaaa cagcttgaag caggagaatg tcgagacatt cgcgccgcat tcacagccga
    34501 ttcgcgctac cttcatcacc gcatagttat catcggttaa cgctggaaca cctgccgcgc
    34561 gcaacgttgc cagcacatct gccagcacat agctgtcctg cggctgctgg cgcgtatgct
    34621 gcaccagcgc ggcatcaggt tgttgctcgt cgacgacctg cttcagtgcc aacaggtcat
    34681 tgaaatcagc agtaataagc gtcagcccca tctgctcaat aataacccgt gtcgtcgggt
    34741 aaacaggcgc gtcatgcacc agaagacgct gccccggttt gagcaaagcc gccagcccgg
    34801 cgcgaatcgc gccagtcccc gcgccctgca ccagcgccgc ggcctgtgcg tgaaatgcat
    34861 cagccagcac ctgctccacc cgttgggtaa tacgcggttg attcagacct ggagttaacc
    34921 ctaaatcacc gccagcaaga aactcgctgc ctggaaaatg gcggcaaata gtgtccacca
    34981 gcgcaaactg cttttgctgc gcttcagcaa gagtcaggct ttgcaaagga aacgtcttca
    35041 taggagcctt ccttaagccg gaacgaacag gccaagccag taaagaacat tcagcagaat
    35101 accggtgatc attactgcca cgaccggtgc cgccattttc tgtaccggac ggcccaggga
    35161 ttcgttgagg aagtaaatgg caaccgcgat agagaatccg gtataacccg ccatcttaat
    35221 tgctgcgaaa atcgaaccga ccagcagcgc cacttccatc agcatattca tggcgttacg
    35281 gatgttatcc gacgcattac gcaccgacgg gtagcgtccc agccatttgc cgatcgaacg
    35341 aagcagcaag acttccgccg aaataaccac tgcgcctaat accgctgcaa ccatcggatt
    35401 cggcgagaga tagcccaccg cataaacaaa ggtaaagccc gcaactgcat acacacccgt
    35461 tgctaaggcg gtggtggcaa tcaacggcac aaaccccagt ccgcgcataa attctgccag
    35521 agccgcctga ttaatcagcg tttgcgattg ttccggcgtc acgcctgcgg aatacgcttt
    35581 ctccagtgtg aagatcgaaa cttcgctacc agcaaaaatc ttcatactgg caacggcggc
    35641 aatcaatgct cccacgatgg cgatataagg taagtttttg ataatccgcg acgtgcgctc
    35701 ttcaaacacc gaaagcccgc tggcatcgat gtcattttca tcacgatgac gcaggtcgtg
    35761 agttatcgcg atccccagca gcatcaccat gccaataaag atttcaatgg attcagggtt
    35821 aagatgtggg aaatagcgca cgactaccac acgggtcatc agaacaacca cggcggcgac
    35881 cagactttgc ttccagccaa actggtagaa aatcgccacc agcgggaaca gtgcaaaagc
    35941 agaaaccacc ggcgagctta attccccgag gctacctaat acatccaccg gcagcgcggt
    36001 cagcagctgg ttaactggca acaggcaagt aaggatcaac acgccccaga tagcgccaag
    36061 accaaacgcc atcaggctgt ttatcgccag tacgccgagg atatcggtag gaagaaagag
    36121 taaccatgcg ttgagcagcc aggttttcag ggtaaaagag atccccaccg aggccacaaa
    36181 accaatgctc agaccaaaag cgatactccc cgcctcgcga cggttcatat agccttcaat
    36241 cagttgcggc aggatcaggt ggatgccgtc atgaaaaaca gccgccgagc gatgcgccag
    36301 aagcgatgtc atacccgtca ggcacgccac cacgataatc tgaatataca gatccatagt
    36361 cgtgcctttt atttcagatg gttaatcaac atcggaatcg cgtgttcgac gtgctcaacg
    36421 gaaaggccaa acgccacttt cccttcagcg atcattttgg cgatatgctc gtctttcgct
    36481 ttaatgcctg gtttggcaat ggtgcaactt ttgttatagc caatcaccgc gatggcaatc
    36541 gacaatgccg cgcccgcacc ggtattacac gcgccaatgt aataatctag ttgcccggat
    36601 ttcaccttca ttgccgcttc catgtcgttg tgaatgaaaa cttcaaaaca gccaggagcc
    36661 gtcgcttcaa tggttttttt aatctgctca cgctgtaagc ctgcaacgcc aatctttttc
    36721 atgagtctgt ccttattgga aaaattgaga gggattttca cgtaacatca catccacatc
    36781 ggcctgactg aatcctgact ggcacaattg cggaataaag gtggtcaata aatagtcata
    36841 gccataacca ccattggctt ttaaatggga gcggcgcgta atatccatcg acagcatgac
    36901 gcggttcagc aacccacggt cacgtagcgc gtggagcatc gcaatgcgct tttcgtccgg
    36961 gtagtaactg ttcttaccaa tggtgtcgaa ctgcacgtac gcgccgagat cgatcatctt
    37021 caaaatgttg tcgaggttgt ctttcagatc gcagtgacca acggtgacgc gcgaaagatc
    37081 aactccgtgg gcttgcaaca gagccagttg ttccagcccc atggtgctga acgacgtatg
    37141 cgtggagatc gggcgtccgg tctggttatg cgccagcgca gcggcaataa ataccttctc
    37201 ttccagcggc gtaatctttc cttcgctgga gccgatctcc gcgatgatcc cggctttcag
    37261 ctccgtgcca tcgataccct gttcaatttc atcgaccatc tcctgcgcca gttcctgcac
    37321 gctgcgggtc accacatgtt ccgggaaaaa cgcatcctgg taataaccag tacaggccac
    37381 cacgttgatc ccggtttcgc gcattacatc gagcataaat tgcgcattgc gccccatgta
    37441 acggttggtc atctcaatca cattacgtac accccgggtc atcaggtcgt tcatctcctg
    37501 gcaaatgaac gcatactgat caaggcggca gtccacgttg tttttaaagc cggagagatc
    37561 aatatgcaga tgctcatggg ccagggtgta acccgtcgga tcaaaactca tagggtgtct
    37621 cctgcaaacc ggagggagga aagaaaagag cgaccatttt gtggcggtgg cgcacggaaa
    37681 aattcacaca cggtagctcc cacatcagaa agcgtggtgc gcacaccgag ctgcgtggcg
    37741 accagccctt gctgataaac cagcactggc accacttcac gggtatggtg gctgtgacca
    37801 atggtcggat cgttaccgtg atccgccatc acgaccaggc aatcatctgg ctgcatcgcc
    37861 tcaacaagcc gggcaaggtt acggtcaacg acctgcaaac gttcggcata acgtgcgacg
    37921 tcttctgcat gaccagcgag gtcggtttcc tggatattga tgcaaataaa cgccgtcgga
    37981 tgggtgttaa attcgtcgag ggtgatatcc ataatccgct ggctatccac caggttttgc
    38041 cagctcacac cataaggatt gctgacgata tctgccacct tacccaccag cacggttggc
    38101 acgcccgctt catacagttt ttgcggcacc tgcacttttt catcaacacc gtagcccata
    38161 tgcacgacct ggaagccgtt gtcataagcg ccagaacgcg gcgcattaat accaataaag
    38221 cgcccttctt tgctttctgc ggcatcgaga atacgctgac tgttggttaa caggccgcca
    38281 aatgtaatga cccgaccgac ctgtacctgg tcacgcacga tacgaccaat tttgattgcg
    38341 tcgtcaaaag agatcacaga gagattggcg gtaatgttat agacttggcc taaatccgcc
    38401 tcgagattat cgccaatcgc aaccgcctga ttgacccaca gaaattgcag atcatcgcca
    38461 cggcgctcca cctgccagcc agcggaaact aatgcctgct caacacggtc aatcacatcg
    38521 cgaaaaggca tccgcagcgg cggtaacggg cgcgtgccta aaatttcctg atgccccata
    38581 aaggtatcgc caccttcatg ttgcagctct gccacgcccc aggttgcgga atctgacggc
    38641 tgcatatcgc ctggcgcata acccaatgtg ttgattagcc ccagcttctc caacgttggt
    38701 agctgcaaat gcggcaactg gctcaggatg tgaccacagg tattcgcccc ggcatcttgc
    38761 ggacgcacca gcgtgacatc tttcattgcc cctacgccaa agctatcaat cactaacacc
    38821 acaaatcgcg ccatttatgc cttcagaggg ttgccgaggc tgtcataacg accaactatt
    38881 tctggttcgc cgcgctgaat accggacacc agcaccacat cgctacgggt gacaaaaatc
    38941 tgcgtgcgaa aacagagcac cactgcactg ctcaccggat actcgcctgc cagcggcagg
    39001 gtgtagtcga tactgctgtc atccacagtt ttgagattgg tttcagtaat cttttgattt
    39061 tctggcgtaa acaccagcgc atgttgcgca tgaccacgac ggtagtaacc gccgccgtag
    39121 cagtagctgt cgccacggaa atgatgggag atttcactta accagagcat cgcgatacgt
    39181 tcaggctgat cgccctgctg gtttgccgga atagtaccgg tcagcgcatg accgggttcg
    39241 gcatgagtcg caccgtattc cgccagcaat ggcagcgaag tgcagctggt cgctgaaggt
    39301 acgttcagtt gctcaatcgc aataccagat ttcgccagtt gatcccgtgc ttgtaccagc
    39361 gtgtgaagat tcggtgttgg caaaactttc ccggcagcct catcccaaag caggcaaggg
    39421 aaatgggtta gtccggctaa atgcagcccc ggcagtttct taatttcagc aatcacctcg
    39481 tgcagcgaat gctgtacaaa accgctctcc tgccccggat aaagaaaatc atcgtcgcta
    39541 taaactttga gcagcacagg ctggacccgt ccggccttaa ccgctgccgc agaaacttcc
    39601 cgcgctttgt cgagagtaaa cacggtgatg atgtcggtgc cctgttcaac ggcgtcagca
    39661 acctgatgac aagggatttg taccagatgc ccctgatgcg ccactggcaa accagcgcgg
    39721 cgcatgactc gcgcctcttt gtaatccacc gccacaatgc cgctgtagcc taatgccagc
    39781 aatttttccg ccagccacgg attacgacca aattgcttgg tcatcagata cagttcaatg
    39841 ccgtaaagcc gcgccgtctc aatgagccgc ttaccgtttt ccagtatctg atccacgtcg
    39901 atcacccagc tgtccggggc gattttgccc tgctgccaca aacttagtgc ggcggaaatc
    39961 agcgccgggt tctggcgttt caatgcttct acaaacatct tgttgcctcg tttctgttcc
    40021 ttgaattaaa tattcatttt tttaaatatt taggttgtgc gacgtggatt tgtcgggagc
    40081 ttcaaacctc ttcgttagca gccatccata atccataaag attggccaga aaatagcctt
    40141 cttcacacgg gttaacttcc agagcgaatt ccttcaacaa cacctgatgc aattgcacaa
    40201 cggcttgcca gtggctggat tgcgccagtt ccgccagcaa ctcgttatcc agcggctcta
    40261 tttcttcacc acggcgactg cgcatcagtg cactcgccat atgtgtcatc gccatcgttc
    40321 cttgctcact gcgcaccggc agatggcact ctttttccag tacgttgacg acctgcatca
    40381 tgcctttgca gacgtcctta tcaataacgc ctgcctcgca aagcaggttg agtctggttt
    40441 ccatatcaga tgcctttcgt ttaataactg ggttcctgtt ccccactcac cacccgttgt
    40501 tgatgggcaa gcagggcgtg tttatctaca acggcacgca gcaattgttg cattgggtaa
    40561 tcatcgactc gcgtcaggac cacggcttcg gtggccagta aaaatcgcgg atcgtctgtt
    40621 agcggcgtcg cctctaatcc cagcatggtc agttcgtttt ccgccaccac gttccagatc
    40681 accgcatcga catcgccttt aacaatgcgt tgtaaactct cgtgataaga gagatcgact
    40741 cgttccacat cactatcgcc aaaaaagaca tcggtcatga ttttctgatc cgccgaacga
    40801 ctatcgagac ctactcgctt aacatttcca gactcacctt tgcggcatat cagttgatgt
    40861 tcaccaacat acgtatgcgg gccaagctcg agggcgatgc acaaattgtt ttggctgaga
    40921 taactttccg ccgccaggcg ggaaactact gccatgtcat agacaccgtt aagtaaacac
    40981 tccacgcgaa tatccgcgcc gcgcatatgc gcatagtaaa aaggaatgcc atcaaactgg
    41041 gctttcaatc cgctcgctaa accttcgtac aagcgggtat agggcaaagg catcgcacat
    41101 accacgttgt tgatatccac atgactcagc aatgctttgt tatccatctc gacaagataa
    41161 ctgccattgc gcccacggcg ttcaatccgt atcgctccgc ttgattccag cgttttcaac
    41221 gcggcctgcg tcaggccaac cgatgaacgg cattcagttg ccagctcatc tatggttttc
    41281 agtcgattac cgcatttttc acccaacaaa taacgggcca gtgtcgtgat gacgacgcct
    41341 tcttttttga taaacgttcg acgcataata tattttcaat aaaatgaata tttatatctt
    41401 cattaaaatg aagataagtg gcgacgacga gatgtggaaa cggcgaggcg cttcacattt
    41461 tttcgggttg ttgcgaaaag aggataaaaa aaccgggttt ccccggtttc agagtgatga
    41521 cgaagtgatt tattcgacaa tgcgagcaaa gggtaattcc cactggcgct tgcctgctcc
    41581 cctcacccta accctctccc caaaggggcg aggggaccgt ccgagcgttt tgatattacg
    41641 gcttcgccac aaaaccaatc gcttcgtaca ccgctttcag cgtacgggaa gcgtgtacac
    41701 tggctttttc tgcgccgtct ttcatcacct gttgcaggaa ggcttcatcg ttgcggaaac
    41761 ggtgatagcg ttcctgcaat tcagtcagca taccggaaac ggcatcagcc acttcacctt
    41821 tcagatgacc atacatcttg ccttcgaact gtttttccag ttctgggatg ctctggcccg
    41881 ttaccgcaga aaggatatcc agcaggttgg aaacgcccgc tttgttctgc acatcgtagc
    41941 gaactaccgg cggctcgtcg gagtcagtga ccgcgcgttt gattttcttc actaccgatt
    42001 tcggatcttc cagcaggccg ataacgttat tgcggttatc gtcagacttg gacatcttct
    42061 tggtcggctc cagcagcgac attacgcgcg cgccagattt cggaataaac ggctccggca
    42121 ccttaaagat ctcgccatac agcgcgttga aacgctgggc gatatcgcga ctcagttcca
    42181 ggtgctgttt ctggtcttca cccaccggta ccagattagt ttgatacagc aggatgtccg
    42241 cagccatcag caccggatag tcaaacagac cagcgttgat gttctcggca taacgcgcag
    42301 atttatcttt aaactgcgtc atgcgactca gttcgccgaa gtaggtatag cagttcagtg
    42361 cccagcctaa ctgcgcatgt tctggtacgt gggactgaac aaaaatggtg cttttctcag
    42421 gatcgatacc acaagccaga tacaaggcca gcgtatccaa cgtcgcttta cgcagcttct
    42481 gtgcatcctg gcgcacggtg atcgcgtgtt ggtcaacgat acagtaaatg caatggtagt
    42541 catcctgcat gtttacccac tgacgcagcg cacccatgta gttaccaatg gtcaattcac
    42601 ctgagggctg tgcgccacta aaaacgatgg gcttagtcat ttttcgattc ctgattttcg
    42661 ctatgcggaa gccctaatgc gggcagaagg tcatttatag actgataaat tacatcaggc
    42721 tggctgagat cgatagcctc gccgtagtta tatccgtagg ttaagccaac tgatgggcaa
    42781 cctgccgctt ttgccgcctg aatatcattg cgtgagtcgc cgacaaacag catctgttgt
    42841 ggggcaattc ccatccgctc agccaccagt aacagcgggt ccggatgcgg ttttttgttt
    42901 tgcacatcat cgccgccgat aaccacgcta aagtatttgg caatatctaa ggcttcgagc
    42961 agcggcgcga cgaacggcgt cggtttgttg gtgaccaggc ctagcggcag gcctttagcc
    43021 tgcaacgcgc ccaacgtatc ggcaacgtgc gggaacaaaa acgtcccctc ttcggcaacc
    43081 tcgccatagt agcgatcgaa cagtttacgc agaatacgta cctgttcttc tgccggaatg
    43141 tcgtcatcaa cgggcggttt ccccattgtt ttacgcagag tcgcacgttc ctgacgcgcc
    43201 caggtcaatg cgcgctccat cagaacatct gcgccgttac caatccaggt aataacgcgt
    43261 tcttcacctg cgacgggcaa ctccagcgca tacagcgcca tatctaccgc agcagcaaga
    43321 ccaggagcac tgtcgaccag cgtaccatca agatcaaaag cgacgccgcg aatatcttca
    43381 aacttattca tgacttacct ttgccagttc actgcgcatt tcatcaatga cttttttata
    43441 gtctggctgg tcgaagattg ccgaaccggc gacgaacata tccgcgcccg ccgcagcgat
    43501 ttcgccaatg ttgttcacct tcacgccacc gtccacttcc agacgaatgt caaagccaga
    43561 ctcgtcgata cggcgacgta cttcgcgcag tttatccagt gtttgaggaa tgaaagactg
    43621 accgccgaaa ccagggttga cggacatcag caggatcaca tccagcttat ccatcacgta
    43681 atccagatag ctcagaggtg tcgccgggtt aaataccaga cccgctttac agccattttc
    43741 tttaatcagt tgcagcgtgc ggtcaacatg ctcggaggct tctggatgaa aggtaatgat
    43801 gctggcacca gcggcagcga aatcaggcac aatgcgatcg acgggtttca ccatcagatg
    43861 tacgtcgata ggggcggtaa tgccgtagtt acgcaaggat ttcagcacca ttggcccaat
    43921 cgtcagattg ggaacatagt ggttatccat gacgtcaaaa tgcacgacat cagcgccagc
    43981 tgccagggct tttgcggtat cttcacccag gcgggcaaaa tcagccgaca gaattgaggg
    44041 ggcaatcaaa tactgtttca tccgcttctc cttgagaatt attttttcgc gggtgaaacg
    44101 actcctggtt tgtacaaagc cagcagttcg tccacctttt tacgtgtgcc gccgttgctg
    44161 cttatactgc gtcgaacttt gacgacatgc aattttgcgc gctgatacca ctcacgcgtt
    44221 aacatcgtat cgtgattgga gatcagcact ggaatatggc gctcaaccag accttcggcg
    44281 atctccgcca gatgcgcttg ttgttcaagc gtaaaactgt ttgtgtgata cgccgtaaag
    44341 ttggcggtcg cagacagcgg tgcataaggc ggatcgcaat agacgacgga tgcatcatct
    44401 gcgcgctcca tgctatcggc gtaagactca caatagaaaa aggcattctg cgctttttca
    44461 gcgaagtgat acaactctgc ttccgggaaa tagggttttt tgtagcggcc aaacggcacg
    44521 ttaaactcac cgcgcagatt gtaacgacac aggccgttgt aaccgtagcg gttcaaatat
    44581 aaaaacagta ccgcccgacg gaacggatcc tggcttttgt tgaactcttc gcggaactga
    44641 tagtaaacct cggcgcaatt tgtttcggga acaaacagct cgcgtgcggc ctgtacgtac
    44701 tcatcagtac gcatcttcac aatgttatag agactgatca ggtcgctatt gatatcggca
    44761 aggatataac gagaaaagtc ggtgttgaga aacaccgacc cggcacctac aaaaggctca
    44821 accagacatt cgcccttggg caaatgccgt ttaatatcat caagcagggg atacttgccc
    44881 cctgcccact tcaaaaaagc gcgatttttc ttcatgccga ctaactaatt acaccttctc
    44941 cggctgtgga gaaagctccg acagcatcca gcgcttcagg cagctcccgc aacgataaac
    45001 cattgcggga gataaccttg attacttcag atcggcctgt acctgacgca gcggtttcgc
    45061 ccacgggttt ttggcctgaa catctgctgg caatgtagat accgcttttt tcgcctcttc
    45121 tttcgaagcg tacacgccag aaaccaggac ataccacggc tgaccattac gcgtcgtttc
    45181 atagacaacg tagtttttca gattctcttt cttcgcccaa ccgttcaggt tgtcgtagtt
    45241 agaggaactg ctcagctgca gagtgtaatg gctggacggt gccgatttca acgaaccaac
    45301 attacctgcg gtcttccctc cagcggctgg tgttgccgtg gtttgcgccg gggatgccgt
    45361 ctgtactgga gccgtagtcg ccgtctcttt tggtgctggc gtagaagtcg cagccggtgc
    45421 cttcgtgctc gccacagggg cagctggttc agtacgcttc ggcgtctgtg ctaccggctt
    45481 cggctccgtt ttcacggttg cttgcggttt tttcggttca atcaccgcct gctgacgaac
    45541 tgggcgcgta gtggacggac gttcagcggt ttgcgttttc gccgtgtcac gcgatgcatt
    45601 gccattgcga acaggcgcaa ccgttgcggg ttcagtcggc aacgtggaat tgaccgccac
    45661 attgttcagc tgttgctgat tttgtggctg ggtcagcgca ttgttcaggt caccctgcac
    45721 ttcaacacgt tgttgaccat ccgttgccac cggggtttgc ccttgagtcg gcgtagaaga
    45781 gatcggtggc agagaaacat cctgctgagt attttccgca gacgtggttc ccggcgctgg
    45841 ctgcacgcca ttcgcctgat cggtcgcatt gccagcaaga tcaatactct tctcgccaga
    45901 cgcggtttga tcgctggaag tggtcgaggg ggcttttagc gcagaaccga taccgatgat
    45961 caacagcagt agaaccagaa tgccgacgcc catcatcata tactgacgag aagcgggttt
    46021 actggctgct tttttgcgct tacgcggacg acgctctacg cgctcttcat ccacggtttc
    46081 atcttcggat tcgtcaattt cttcttcgat ttccggttcc tcattgcgct ctttttgcgc
    46141 acgagtcgga cggcgatcgt cagtgtcatc aagttcaata tcatcaaaat tgatctgcgg
    46201 ttcgccacgt tcagtacgct cagaacgttc agaagattga cgagaacgac cagtacgacg
    46261 atcgctggga tcgggtttca gctcgtcttc tggtttgaat tcatccattt aacaccccac
    46321 taaaaggtta atgcttacca cgttgcaatt aacctgaagc taatagaccg cttgataagc
    46381 ggcctgacct ttcttgttgt tacgctgatt gacaatcggc aatggcgtta agaacaagct
    46441 cgtgcgaaac gccgctgcga acttcactct taccaattgc caacggaaga attaagcgca
    46501 tctctcccgc aaggactttc ttgtcacgca gcatatgcgg taaatacgcc tgcgcggaca
    46561 tttcgcgcgg cccattgacc ggtaacccag cccgcgtgag cagggttata atacgctgcg
    46621 tttcggcaga actaaactgc ccgagacgtt ccgacgtccg cgccgccatc accatacccg
    46681 cagcgaccgc ttcaccatgt aaccaattgc cataccccat ttcagcttca atggcatgac
    46741 caaaggtgtg tcccagattc agtaaagcac gtaacccggt ttcgcgctcg tcggcggcga
    46801 caacttctgc cttcagttca caacaacggc gaatacagta cgccattgcc ggaccgtcca
    46861 gacgcaacaa cgcatccaga ttctcttcca gccagttaaa aaacgcaccg tcaagaataa
    46921 tgccgtattt gatgacttct gccagccccg acgctaactc acgcggggga agcgttttca
    46981 gacagtcgag atccaccacc actgaagcag gctggtagaa cgcgccaatc atgtttttac
    47041 cgaggggatg gttgaccgca gttttgccgc caacggagga atcgacctgc gacagtaacg
    47101 tcgtcgggac ttgaatgaaa cgaacaccgc gctgataact cgccgccgcg aagccggtca
    47161 gatcgcccac tacgccgccg ccaagcgcca ccagcgtagt atcgcgacca tgcggctttt
    47221 gtaacaacgc cgtaaagacg gtatcgagta cagctaggct tttatactgc tcgccgtcag
    47281 ggaggataac gctatcgacg ttaacacccg cctgttcaag tacgccgcgg accttatcga
    47341 gatacagagg agccagggtt tcgctggtga ccaacatgac ctgctcgccc gatttcagcg
    47401 gtaagaatga agctggttca ttaaacaaac cagatgcgat ggtaattggg taactacgtt
    47461 ccccgagagt aacgacaatc ctctccataa cgcgacatcc accttaatta ctgtacccgc
    47521 agacgagtgt atataaagcc agaattagtt gctttccagc atgtgaataa tctggtttgc
    47581 aaccacttta gcgctttgat catcagtacg aatggtcacg tcggcaatct cttcatacag
    47641 cggattgcgt tcattggcca acgcttccag aacttcacgc ggcggtgttt caacgtgcag
    47701 caacgggcgt tttttatcac gctgcgtgcg tgcaagttgc ttttcgatgg tcgtttcaag
    47761 ataaacgaca acgccacgag cggaaagacg gttacgcgtt tcacgggatt tcacagagcc
    47821 gccgccagta gccagcacaa taccctgttt ctcggtcaac tcattgatga ccttttcttc
    47881 gcgatcgcgg aagccttctt cgccttctaa atcgaaaacc cagcccacat cagctccggt
    47941 tcgtttctca atctcttgat cggaatcgta aaattccata ttgagttttt gagctaactg
    48001 gcgcccaata gtgctttttc cggcacccat aggcccaacc agaaagatat tgcgtttctc
    48061 tgccattttt tcggtactac taagactatt cgttaatgat aaacccgctt cgctcagaga
    48121 gcgccgcagg acatgaactg aaacctcata agatattgcg agagtcagac tgaaaattat
    48181 ctcaatactc cagcgggttt ggcaactgaa taaatcaccc atccctctgc atatctggtc
    48241 gctgcaagcg cactgccttg ctaccaccgc tctggcgata aatcaccggg taagattagc
    48301 gtaaaaaaga cagcaaaatg ccgcctgaat gataaatcat catcatggaa cgcaggcagc
    48361 gaagagcacc aactctcaaa tcggtactcc ttgtatgcta aatacctgcg cgcgtcaaat
    48421 agatgaaaca cgttcagcat aaaaacatta ccgcttttac ggctgtttac tcactggaaa
    48481 ccagtcgtgg cgtgataaac accactaact cgcgtcgttc atcttctttt ccgtcatgac
    48541 gaaataattg cccgaaccag ggaatgtcgc caagcaacgg tacgctatcc tgacccgatt
    48601 tatttttacg ggtaaaaatg ccgcccagcg ccaacgtttc tccgcttttt acctcgacct
    48661 gcgtttcgat ctcctgctta tcaatcgcca gcacttcgcc atcggcctgc tgtagcacct
    48721 gccccggaac gttctggctg atgtgtaatt tcagccggat gcgacctttt tgtaacaccg
    48781 tgggcgtgac ctccatcccc aggacggcct ctttaaattc caccgacgtc gcgccacttt
    48841 ccccgctgga agcctgatat ggaatttcgc tcccctgttt aatgctggca ggctgaagat
    48901 gtgaggccag cagacgcgga ctggcgataa tatccagctg ctgtttttgt tcgagcgcgg
    48961 aaagctcaag atccagcaag cgtccgttga tgcgcccaat gttaaaaccg acatgcgttg
    49021 tcgccgtcgc cacggagagg tcgctaccaa gcgtggtgac ttgcccaacg ccaccagcgt
    49081 gttgcgcatc ggccagcgtc catttcacgc ctaactcacg caaacttttt tcattaatgg
    49141 tgacaatatg cgccgacagc tcaacctgcc cgaccggcag atccatttgc gctacccact
    49201 gttcaagcgc gcttaacgcc gttttgttat ctcgtagcaa aaggcgattg gtgcgtttat
    49261 cgacggtcat actccctttg gcactcagta gcttctcccc cgctttcgcc agttctcccg
    49321 cgtcggcgta ttgcagggtt atactgcgat tttccagcgg cagatttgcc tgcgcccgcg
    49381 cctgctccgc ctcctggcgg gcgatattgt cattctgcca ggcaatggaa tgcactgaga
    49441 gaatgttgcc ttcctgccgc gttatcagtc cggcgctttt cactacagtt tgtagtgcct
    49501 gcttccaggg aacatctgtt agatgtaacg acaccgtacc gctgacgtct ggcgacacga
    49561 ccaggttcaa cttctcctgt tcagccagcg cctgcaacac ctgagctacc ggaacgtcat
    49621 ccaccatcag cgtcactttt tgcggctttg ccgcctgtac gccgggtatc agcatcaaca
    49681 gtagtgcggc tatccattgc ttcatttgta tctccttgcc gttgccacaa ccattgtggc
    49741 ggttcgcagt ttgtcccggt acccagcgtt agtacgtctg gcgtcagctg taaaattgtc
    49801 cagccgtttt ccagcacatc gttttgctgc acccgtcgcc atttcttttg cccgtctttt
    49861 attacaccga tgatgcgctc gcctcgccct accatcccct gatagcgcca ctggctaagt
    49921 tcgctaatcc ggcatagatc ttccggcgat ttaaaagggt cacgcatacc ggttaaaagg
    49981 cacaatgcaa tacctgccaa caaccagcgt ttaaccctca tgcggcatct ccagttgtag
    50041 cgtgaacaaa agatcatcac cttccacgct taacgaaaaa cggctcacgc tgacgttgcg
    50101 ctctgccagc cgtgtaaatg ccgacggcac tgcttcccac agcgttttca acgccaactc
    50161 gcctccctgc gcggatggat gccaggaaac cagttgcgcg ccggataact gaaaatccag
    50221 tggcgaaaag ggcagcgttt tttcctcgct gaagggagtg gtgtctacca ggcgatacag
    50281 tgcggcccac tgctggtgat gacttgcccg tagtcgaatt aatgcgtcgc gctcttcatg
    50341 gtgtgtcgat gatagaaaaa tgagcgtaac taacatcagc aaccagaatg cccagcaaaa
    50401 ctggcggagg cggggtgatg tggcgaacca ccagtcaaag aacatgttca tcgctaacct
    50461 tccttgttaa ctgatactca aattgccagc gtccctgcgc atcctgctgc gtggctcccc
    50521 gctgattgag atgaaaagac gcatcctggc ggagtgacgt ttctagtgcg tttaacgcgg
    50581 taatgcttgt tgtcagcccc ttgatctcca gcgttccctg ctgccagctt atcgttgtca
    50641 gccaggcgtg ctcgggtaaa agcgccgcca gtgcttccag cgcagattgc cagtcgcggg
    50701 taaattgtcg ctggcgctgc cgttgcgaac gctgttcgcg taactgctgt tgctccagca
    50761 aacgtggctt cgttatctgt aagctgcggg cgagttgttg ttccgcctga agcaaaactg
    50821 cgtctatgcg cgcttcggcg ctgcctgtca gacgcagtat tagcgttatc ccgacggcca
    50881 gcagcagagg cgcaacgaac atcagcaacc agaaacgcag aaaagcggtc cggcgttgct
    50941 gtcgccaggg caaaaaatta attggcgggt tcatcagtac gccttcccaa gcgccagccc
    51001 cagcgcgatg gcaaagtctc caccaggcgg cggtagcggc ggctgacgaa cagaaacggc
    51061 ctcccagggg tcaaatccgc cttcgccaca tatcgcgacg ctgtcgggat caacggataa
    51121 cgctgccgcc agctccttcg cgctagtcat ccccaccgcc agtttgcgcc cccagctata
    51181 gcgcgtcgcc cacagccact gttcgttatc acgccaggcc agacattgct gatgagaagg
    51241 taaaaaaggc aggaatcgct gtaatgcact ggcatccggg gtgatcgcac taacatgaac
    51301 acgcaacctt tctgccagcg taagcagcgt tgccagctct ttgctttgcg cggcagtcac
    51361 gttataagcg gggctgagtg agtcttcgct gtaatcgaag cgcagggagt ccggatccat
    51421 atccagctcg cgggccatcg tccctgacag ccaggccgtt tgctcccgct caccaaggga
    51481 catcgacggg cgcggaaatg accgctgtaa tgtgcgactg gcgggaaacg ccaacataat
    51541 gtgatgacgc tgcggcagtt cgcgactcca cggtaacaac gttttagcca gctgctgcgc
    51601 atcaacaatc cgcccatctt tgataatgtc gtgcgccagc ggcaaccgcc accagcgttg
    51661 caaaaagcat tcttttgcgc cccgcacgat cgcaaccgct gccgcttctt gctgttgtaa
    51721 atgcaaacca atttgccaga tcttaaatgt cattgtgatg atctccttat cactcgtcac
    51781 tctgacgggt atatcaatgc gtctggcttg cctttatact accgcgcgtt tgtttataaa
    51841 ctgcccaaat gaaactaaat gggaaatttc cagtgaagtt cgtaaagtat tttttgatcc
    51901 ttgcagtctg ttgcattctg ctgggagcag gctcgattta tggcctatac cgctacatcg
    51961 agccacaact gccggatgtg gtgacattaa aagatgttcg cctgcaaatt ccgatgcaga
    52021 tttacagcgc cgatggcgag ctgattgctc aatacggtga gaaacgtcgt attccggtta
    52081 cgttggatca aatcccaccg gagatggtga aagcctttat cgcgacggaa gacagccgct
    52141 tctacgagca tcacggcgtt gacccggtgg ggatcttccg tgcggcaagt gtggcgctgt
    52201 tctccggcca cgcgtcacaa ggggcgagta ccattaccca gcagctggcg agaaatttct
    52261 tcctcagtcc agaacgcacg ctgatgcgta agattaagga agtcttcctc gcgattcgca
    52321 ttgaacagtt gctgacgaaa gacgaaatcc tcgagcttta tctgaacaag atttaccttg
    52381 gttaccgcgc ctatggtgtc ggtgctgcgg cacaagtcta tttcggtaaa acggtcgacc
    52441 aactgacgct gaacgaaatg gcggtgatag ccgggttgcc gaaagcgcct tccaccttta
    52501 atccgctcta ctcaatggat cgtgccgtcg cgcggcgtaa cgtcgtactg tcgcggatgc
    52561 tggatgaagg gtatatcacc cagcaacagt tcgatcagac acgcactgag gcgattaacg
    52621 ctaattatca cgcgccggag attgctttct ctgcgccgta cctgagcgaa atggtgcgcc
    52681 aggagatgta taaccgttat ggcgaaagtg cctatgaaga cggttatcgc atttacacca
    52741 ccatcacccg caaagtgcag caggccgcgc agcaggcggt acgtaataac gtgctggact
    52801 acgacatgcg ccacggctat cgcggcccgg caaatgtgct gtggaaagtg ggcgagtcgg
    52861 cgtgggataa caacaagatt accgatacgc tgaaggcgct gccgacctat ggtccgctgc
    52921 tgcctgccgc agtcaccagc gccaatcctc agggagcgac ggcgatgctg gcggacgggt
    52981 cgaccgtcgc attgagtatg gaaggcgttc gctgggcgcg tccttaccgt tcggatactc
    53041 agcagggacc gacgccgcgt aaagtgaccg atgttctgca aacgggtcag caaatctggg
    53101 ttcgtcaggt tggcgatgca tggtggctgg cacaagtgcc ggaagtgaac tcggcgctgg
    53161 tgtcgatcaa tccgcaaaat ggtgccgtta tggcgctggt cggtggcttt gatttcaatc
    53221 agagcaagtt taaccgcgcc acccaggcac tgcgtcaggt gggttccaac atcaaaccgt
    53281 tcctctacac cgcggcgatg gataaaggtc tgacgctggc aagtatgttg aacgatgtgc
    53341 caatttctcg ctgggatgca ggtgccggtt ctgactggca gccgaagaac tcaccaccgc
    53401 agtatgctgg tccaattcgc ttacgtcagg ggctgggtca gtcgaaaaac gtggtgatgg
    53461 tacgtgcaat gcgggcgatg ggcgtcgact acgctgcaga atatctgcaa cgcttcggct
    53521 tcccggcgca aaacattgtc cacaccgaat cgctggcgct gggttcagcg tccttcaccc
    53581 caatgcaggt ggcgcgcggc tacgcggtca tggcgaacgg cggcttccta gtggacccgt
    53641 ggtttatcag caaaattgaa aacgatcagg gcggcgtgat tttcgaagcg aaaccgaaag
    53701 tagcctgccc ggaatgcgat attccagtga tttacggtga tacgcagaaa tcgaacgtgc
    53761 tggaaaataa cgatgttgaa gatgtcgcta tctcccgcga gcagcagaat gtttctgtac
    53821 caatgccgca gctggagcag gcaaatcagg cgttagtggc gaagactggc gcgcaggagt
    53881 acgcaccgca cgtcatcaac actccgctgg cattcctgat taagagtgct ttgaacacca
    53941 atatctttgg tgaaccaggc tggcagggta ccggctggcg tgcaggtcgt gatttgcagc
    54001 gtcgcgatat cggcgggaaa accggtacca ctaacagttc gaaagatgcg tggttctcgg
    54061 gttacggtcc gggcgttgtg acctcggtct ggattggctt tgatgatcac cgtcgtaatc
    54121 tcggtcatac aacggcttcc ggagcgatta aagatcagat ctcaggttac gaaggcggtg
    54181 ccaagagtgc ccagcctgca tgggacgctt atatgaaagc cgttcttgaa ggtgtgccgg
    54241 agcagccgct gacgccgcct ccgggtattg tgacggtgaa tatcgatcgc agcaccgggc
    54301 agttagctaa tggtggcaac agccgcgaag agtatttcat cgaaggtacg cagccgacac
    54361 aacaggcggt gcacgaggtg ggaacgacca ttatcgataa tggtgaggca caggaattgt
    54421 tctgattaaa aaggcgcttc ggcgcctttt cagttggctg acaaagtgca tttgtttatg
    54481 ccggatgcgg gtgaacgcgt tatccggcta acaaaatcgt gaaaaatcaa tatattgcag
    54541 gaaccccata agcctgataa gcgttgtgca tcaggcaaac ttcacgcatt tacactcgcc
    54601 cctgcccttt caaccattcg cgcacgagga acagcgcgct gacattacgc gcttcgttga
    54661 agtcagggtc ttccagcaaa tccatcatat gcgccagcgg ccagcgcacc tgtggtagcg
    54721 gctctggctc atcgccttcc agcgattccg ggtagagatc ttgcgcgacc acgatattca
    54781 ttttactgga aaagtaagac ggtgccatgc tgagcttctt caaaaaagtc agatcgttcg
    54841 caccaaatcc aacctcttct tttagctcgc ggttagcggc ttcgtagacg ctttcacccg
    54901 gatcaattaa tcctttcgaa aaacctaatt cgtaggattc agttcccact gcgtattcgc
    54961 ggatcaggat caggtgatcg tccacaatcg gcacaatcat cactgcttcc cggttggttg
    55021 gacgcattcg ttcataaaca cgccgcacgc cattgctgaa ctccagatcc acactctcga
    55081 cggtaaacag tcgggaacgg gctacagttt caacattcag aatggtgggt ttttgtaatg
    55141 atttgttcat cgtgggatct atgctgtgaa atcagcggtt attgtgcgat atcggacacg
    55201 ctttcggcaa tgtgaattgc atgttattta catttatgta acttaataaa taatcgtcct
    55261 caaatcaaat taaaagtcaa taggttgaaa taactccagg aatttgctga tattccgcct
    55321 tcagagggtt tgctatgatc agcggttact gtgatgtgct taatgatgct caagttaaac
    55381 tccacgcttg ccgatagcca accgcagaat catgtattgt gtccggtgcg actgaccacg
    55441 cctgacagac taagtaagat ggggaaagca tgagcaccat tgtgattttt ttagctgctt
    55501 tgctggcctg ctcactactt gcgggatggc tgataaaagt gcgatccaga cagcgtcagc
    55561 tgccctggac caacgccttc gcggatgcgc aaacgcgtaa actcacacct gaagaacgta
    55621 gcgccgttga aaattatctt gagagcctga cgcaggtatt acaggtgcct ggcccaacgg
    55681 gagctagcgc ggcaccgatc tctctggtgc tgaatgccga aagcaacaac gtcatgatgc
    55741 tgacacacgc tatcacacgt tacggcatct ctaccgacga tccgaataaa tggcgttact
    55801 acctcgattc ggtagaagtc cacctgcccc ccttctggga acagtacatc aacgatgaga
    55861 ataccgttga actgattcat accgattcgc tgccgttggt tatttcactc aacggtcata
    55921 cgctgcagga gtacatgcag gaaacgcgcg gctatgcctt gcaacctgtt ccgtccacgc
    55981 aggcgtcgat tcgcggggaa gagagtgagc aaatcgagct actcaatatt cgcaaagaaa
    56041 cgcatgaaga atatgcgctg agtcgtccgc gcgggctgcg tgaagcgttg ctgatcgtcg
    56101 cctccttcct gatgttcttt ttctgcctga ttaccccgga tgtatttgtt ccgtggctgg
    56161 caggcggcgc attactgctg ctgggcgcag gtctgtgggg gctattcgcg cccccggcaa
    56221 aatcctccct gcgagaaatt cattgtctgc gcggtacacc ccgtcgttgg ggattgtttg
    56281 gcgaaaacga tcaggaacag atcaacaata tttcactcgg tattatcgac ctggtctatc
    56341 ccgcacactg gcagccatac attgctcagg atctcggtca acaaaccgat attgatatct
    56401 atctcgaccg ccacgtagtg cgtcaggggc gatatctttc gctgcatgat gaagtaaaaa
    56461 actttccgtt acagcactgg ctgcgcagta cgattatcgc tgcgggctcg ctgctggtgc
    56521 tgtttatgct gttattctgg atcccgctgg atatgccgct gaaattcact ctctcatgga
    56581 tgaaaggcgc gcagaccatt gaagccacca gcgtaaaaca actggctgac gctggcgtgc
    56641 gggtaggcga tacattgcgt attagcggta cgggaatgtg taatattcgc acttccggaa
    56701 cctggagcgc gaaaaccaat tcaccttttt taccatttga ctgctcgcag atcatctgga
    56761 atgacgcccg ctcattacca ttaccagaat ctgaactggt caacaaagcg acggcattga
    56821 ctgaagcagt taatcgccag ctgcacccta aaccggaaga tgaatctcgc gtcagtgcct
    56881 cattacgttc agcgattcaa aaatccggca tggtattgct tgatgatttt ggcgacattg
    56941 tactgaagac agcggattta tgttctgcca aagatgactg tgtgcgactg aaaaatgcgc
    57001 tggtcaatct cggcaacagt aaagactggg acgcgctggt aaaacgcgcc aacgctggga
    57061 agctcgatgg cgtgaatgtg ttattacgcc cggtgagtgc ggaatcgctg gataacctgg
    57121 tggcaacctc caccgcgccg tttatcacgc atgaaacggc gcgagcggca caatcactaa
    57181 acagtccggc ccccggcgga ttcctgattg tcagcgacga aggcagcgat tttgttgatc
    57241 agccctggcc ttcggcatca ctttacgact acccgccgca agaacagtgg aacgctttcc
    57301 agaaactggc acaaatgctg atgcatacgc cgtttaacgc cgaaggtatc gtcacaaaaa
    57361 tcttcactga cgccaatggt acgcagcata ttggccttca tccgatcccg gatcgttccg
    57421 gcctgtggcg ctatctcagc accacattgc tgctactgac gatgctgggt agcgccattt
    57481 acaatggcgt acaggcctgg cgtcgttacc agcgtcatcg cactcgcatg atggagattc
    57541 aggcctatta tgaaagctgc ctgaacccgc aactgatcac cccttcagaa agccttatcg
    57601 aataacacgt ttgcgcggca ggttatgcta ccctgtcgcg caaattgctt cactctggag
    57661 atttccctca tgcatatcaa cattgcctgg caggacgtag ataccgttct gctggatatg
    57721 gacggcacgt tgctcgacct cgccttcgat aactatttct ggcaaaagct ggtgcctgaa
    57781 acatggggcg cgaaaaacgg ggttacgcca caggaagcga tggaatatat gcgccagcaa
    57841 tatcacgacg tacagcatac gctaaactgg tactgtcttg attactggag tgagcaactg
    57901 ggtctggata tctgtgcgat gaccaccgag atgggaccgc gcgccgtact gcgtgaagat
    57961 accattccgt ttcttgaggc actgaaagcc agcggtaagc agcgaatttt gctcaccaat
    58021 gctcatccgc acaacctggc ggtaaaactt gagcatacgg gtctggacgc acaccttgat
    58081 ttattacttt ccacccacac atttggttat ccgaaagagg atcagcggtt atggcatgcg
    58141 gtggccgaag ctacgggtct gaaagctgaa agaacgctgt ttattgatga cagcgaagcg
    58201 attctcgatg ctgccgcgca atttggtatt cgttactgcc tcggcgtgac taatcctgat
    58261 tccgggattg ccgagaaaca gtatcaacgc catccgtcac tgaatgacta ccgccgcctg
    58321 atcccctcgc taatgtgaag gagacgccat gaaagagaaa cctgctgttg aggttcgact
    58381 ggataaatgg ctatgggctg cccgttttta taaaacccgc gcgctggccc gtgaaatgat
    58441 taaaggcggt aaggtgcatt acaacgggca gcgcagcaag ccgagcaaaa tcgtcgagct
    58501 gaatgccacg ctcactctgc gccagggaaa tgacgaacgc acggtgattg taaaggcgat
    58561 tactgaacag cgtcgccccg ccagcgaggc agccttgctg tatgaagaga ctgcggaaag
    58621 tgtagagaaa cgcgaaaaaa tggcgatggc acgtaaactt aatgccttaa ccatgccgca
    58681 cccggaccga cgcccggaca aaaaagagcg ccgcgacctg ttacgattta aacacggcga
    58741 cagtgaataa ctgtcacctg caagagagat gattatgccg caacatgacc aattacatcg
    58801 ctatctgttt gaaaattttg ccgttcgcgg cgaactggta accgtttcgg aaaccctgca
    58861 acagatcctt gagaaccacg attatccgca gcccgttaaa aacgtgctgg cagaactgct
    58921 ggttgcgacc agcctgttaa ccgctacgct gaagtttgat ggtgatatca ccgtacagct
    58981 gcagggcgat ggtccgatga atctggcggt tattaacggt aacaataacc agcaaatgcg
    59041 cggtgtggcg cgcgtgcagg gcgaaattcc agaaaatgcc gacctgaaaa cgctggtcgg
    59101 caatggttac gtggtgatca ccattacccc gagcgaaggc gaacgctatc agggcgtagt
    59161 tggtctggaa ggtgataccc tggcggcctg cctagaagat tactttatgc gttctgaaca
    59221 gctgccgacg cgcctgttta ttcgcaccgg cgacgtagac ggcaaaccgg ctgcaggcgg
    59281 tatgttgttg caggtaatgc ctgcgcaaaa tacccagcag gacgactttg accacctggc
    59341 gacgctaacc gaaaccatca aaaccgaaga actgctgacc ttaccggcaa acgaagtgtt
    59401 gtggcgtttg tatcacgaag aagaggtgac ggtttacgat ccgcaggatg tggagttcaa
    59461 atgcacctgc tcgcgtgaac gttgcgccga tgcgctgaaa acgctgcctg atgaagaagt
    59521 tgatagcatc ctggcggaag atggcgaaat tgacatgcat tgtgattact gcggtaacca
    59581 ctatctgttc aatgcgatgg atattgctga aatccgcaac aacgcgtctc cggcagatcc
    59641 gcaagttcat taatgagtat gtccggcaga gagggctctg ccggatttta cgacttaatg
    59701 gcctgatgtg ctgcgcttac cagcgcctat gaaataaatc gcaaattatt ttggtcagat
    59761 acagcgtcgt atccgataaa agcgttgtca cttacttaag tttatttcca gcgtttcacc
    59821 atctaccgtc agatcgcgcc acgctttcgg cctcgtcccc agttttccat ccttcgcagc
    59881 tgtgagtaat ttttctttcg ttatttcgtt cggaagtaaa ctcatatcaa gcgcgtccca
    59941 ttcttttctg tctgagtcaa agccatagac aatgactctg tcatcattaa aagcaaacag
    60001 gatccgctcc gcctgaccat cactattcag atcttgctcg accagtacgc aggcgtcttt
    60061 ttcaatacag gtcatcacgt tatagcgatc ctggattaag gccgaccaga atgtcgcatc
    60121 cggtttaaca gaaccagggg caattaacac attttcggct aatacttttt ccgatacctg
    60181 ttcctgaaga tgttgctctc catctaacgc catcagcaga tcccgcgcgc gtttcgggtc
    60241 tttcataaac ccggcatcgc ttttcagcga ctcaagcgcc gcacgtccat agcgaccgct
    60301 ctgttcgagc atgtagatag ttacctggtc tggcgtgttt ttgccgctct gataacgcgc
    60361 catatgactg ttcacgctaa tacgcatact gtccagcacc ggcgaattaa gaagcaccag
    60421 tatcaccaac accaataacg aaaccgcaag gttcactttg ccctgaagaa caaggggatt
    60481 ttgcccatta cgccagacga tactgacaaa ataccccagc gaccacacca gtaacaccag
    60541 ctccgccagc gcgccctgca agcggtcgac agtccagccg tactgagcga cccgcagcca
    60601 taacccccag gtggcgacga acacatacag cggagcaacc agcaaagcgg ttttaatcag
    60661 gcaacgcaac ggccccgtcc agggaagtga cgctttttgc ggatcgcgga caatagccat
    60721 taagatcaat tgcaaaaagg ccagcgttaa cagcaacccg gcggcggaga tgtggcgaga
    60781 aatcgcgctc aggcccgtaa acggcagggt gatgataaac atcagggtta gcaatgatac
    60841 taacggcagc aaccccgtgg cgattagcgt gaacaacttt tgaatagagt cgattaaacg
    60901 tgactgtgta cgcgcgagga tcaccgccag tgcggtaacc agaccaaatg taagatacat
    60961 aaaccagtcg gttgcaaaaa agagcgtatt gaaaaacgtg atgccaacaa gtttaaacaa
    61021 ctcaccccac agtaaaagca ccagccacgt caggccattg gagagaaaaa tcaccagtaa
    61081 tataagtacg ttatgccata ctgactggta aaagtagcta tagcggctac tgccattgcg
    61141 gatacgaagg ctttgttgta tccacggtag caacaacatt gccatcaaca gcagatagca
    61201 gccaaaatcc caaatcgcct tttcagctct ccaggggttc atgccatcag tttgccactt
    61261 cagccaaccg ctcatccctg tcgtggcaat aaacaccagc gccagccatc cccaaaggcg
    61321 cttctgttta aaagagatca cggagaagag taaaacggat gaaaacgcga cggtcgcagg
    61381 cacgccataa acaatccaac tgtgattttt tccggcaagc caggcgataa gcaggtagca
    61441 aaccagcccc tgaagtaatc cggtagcaat catcccccag cgtgttgccg gtgaaagttc
    61501 aacgttatcc atgttggtta tccagaatca aaaggtgggt taattatcgc atccgggcag
    61561 tagtattttg cttttttcag aaaataatca aaaaaagtta gcgtggtgaa tcgatacttt
    61621 accggttgaa tttgcatcaa tttcattcag gaatgcgatt ccactcacaa tattcccgcc
    61681 atataaacca agatttaacc ttttgagaac attttccaca cctaaaatgc tatttctgcg
    61741 ataatagcaa ccgtttcgtg acaggaatca cggagttttt tgtcaaatat gaatttctcc
    61801 agatacgtaa atctatgagc cttgtcgcgg ttaacacccc caaaaagact ttactattca
    61861 ggcaatacat attggctaag gagcagtgaa atgcgcgtta acaatggttt gaccccgcaa
    61921 gaactcgagg cttatggtat cagtgacgta catgatatcg tttacaaccc aagctacgac
    61981 ctgctgtatc aggaagagct cgatccgagc ctgacaggtt atgagcgcgg ggtgttaact
    62041 aatctgggtg ccgttgccgt cgataccggg atcttcaccg gtcgttcacc aaaagataag
    62101 tatatcgtcc gtgacgatac cactcgcgat actttctggt gggcagacaa aggcaaaggt
    62161 aagaacgaca acaaacctct ctctccggaa acctggcagc atctgaaagg cctggtgacc
    62221 aggcagcttt ccggcaaacg tctgttcgtt gtcgacgctt tctgtggtgc gaacccggat
    62281 actcgtcttt ccgtccgttt catcaccgaa gtggcctggc aggcgcattt tgtcaaaaac
    62341 atgtttattc gcccgagcga tgaagagctg gcaggtttca aaccagactt tatcgttatg
    62401 aacggcgcga agtgcactaa cccgcagtgg aaagaacagg gtctgaactc cgaaaacttc
    62461 gtggcgttta acctgaccga gcgcatgcag ctgattggcg gcacctggta cggcggcgaa
    62521 atgaagaaag ggatgttctc gatgatgaac tacctgctgc cgctgaaagg tatcgcttct
    62581 atgcactgct ccgccaacgt tggcgagaaa ggcgatgttg cggtgttctt cggcctttcc
    62641 ggcaccggta aaaccaccct ttccaccgac ccgaaacgcc gcctgattgg cgatgacgaa
    62701 cacggctggg acgatgacgg cgtgtttaat ttcgaaggcg gctgctacgc aaaaactatc
    62761 aagctgtcga aagaagcgga acctgaaatc tacaacgcta tccgtcgtga tgcgctgctg
    62821 gaaaacgtca ccgtgcgtga agatggcact atcgactttg atgatggttc aaaaaccgag
    62881 aacacccgcg tttcttatcc aatctatcac atcgataaca tcgttaagcc ggtttccaaa
    62941 gcgggccacg cgactaaggt tatcttcctg actgctgatg ctttcggcgt gttgccgccg
    63001 gtttctcgcc tgactgccga tcaaacccag tatcacttcc tctctggctt caccgccaaa
    63061 ctggccggta ctgagcgtgg catcaccgaa ccgacgccaa ccttctccgc ttgcttcggc
    63121 gcggcgttcc tgtcgctgca cccgactcag tacgcagaag tgctggtgaa acgtatgcag
    63181 gcggcgggcg cgcaggctta tctggttaac actggctgga acggcactgg caaacgtatc
    63241 tcgattaaag atacccgcgc cattatcgac gccatcctca acggttcact ggataatgca
    63301 gaaaccttca ctctgccgat gtttaacctg gcgatcccaa ccgaactgcc gggcgtagac
    63361 acgaagattc tcgatccgcg taacacctac gcttctccgg aacagtggca ggaaaaagcc
    63421 gaaaccctgg cgaaactgtt tatcgacaac ttcgataaat acaccgacac ccctgcgggt
    63481 gccgcgctgg tagcggctgg tccgaaactg taatgatttg aagctggaga atatctatcc
    63541 agtatcttat agaaagcaaa acgggaggca ccttcgcctc ccgtttattt acccttcttt
    63601 tgtcgtgccc tgcgcccgcg ttaccggcac tggcagccag gcgcgaatgg aaagcccgcc
    63661 ccgctcgctg gtgccaagct ccagcatccc gttatggtta tccacgatac gctgcacaat
    63721 tgccagccct aatcccgtgc cgctaatggt gcgcgcactg tcgccgcgga caaacggctg
    63781 gaacaggtgc ttacgttgtt ccggcgcaat tcccggaccg tcatcttcca cctggaacca
    63841 ggcgcgattc ggctccgttc cgctgctgac tttgatccag ccattgccat aacgggcggc
    63901 gttgaccacc atattcgcca ccgcgcgttt gatcgacagc gggtgcattt tcacttcaat
    63961 gctgccgggg taaagcgcgg tttcaatttc ccgctcatag ccactttcgg cagcaatcac
    64021 ctcaccgagc actgcattaa gatccgccat ttccatcggc atctcctgcc cggtgcgcag
    64081 gtagtcgata aactgctcaa tgatggcgtt gcactcttcg atatctttat tgatcgattc
    64141 tgccagatag ccatcctgct cgctcatcat ctcagtcgcc aggcgaatac gcgtcagcgg
    64201 cgtgcgcaag tcgtgactta cccccgccat cagcagcgtg cggtcatccg ccagttgctt
    64261 aacaccagcc gccatatggt taaaggcacg ggtaacggaa cgcacctccg aagcgccata
    64321 ttcacgcagc ggcggcggaa taatcccttt accaacctgc aaggctgcgt gttcgagatc
    64381 gaccaacggt cggttctgga tacgaataaa cagccacgcc ccgcctatcg ccaatagcat
    64441 aatcgccagc gtatagcgga acagcggaga gaaatcgccc tgatgaattt cggtcagcgg
    64501 cacgcgtacc cagatattgg gcgacagcca ggttttcagc cagacgacag gcgaactttt
    64561 gttgacctca acgcgcactt ccgtcgggcc gcccagttgc tgtgccatct gatggcttaa
    64621 gaattcatag tgttgcgccc aacgcagacc tgcctcttcg gcagcctcgt tggagtagag
    64681 agagatcccc agctcacggt agatctcccg acggaaagcg ggaggcacaa ccaactgcgt
    64741 gccgtcctcc agttgcagtt tatcggtcat caacatacgc acttcgtacg cgaggacttt
    64801 attaaactgc tggaggctcg gcaaaatcgc gaagttcagc accaccagat aagtcgtcac
    64861 caggctggcg aacagcaagg tgacgatgag caataacgta cgggcaaatg aacttcgtgg
    64921 cgagaagcgc aatcgcctca tgctttagag ccgtccggta caaagacgta gcccaggccc
    64981 caaacggtct gaatgtaacg cggatgcgct ggatcttctt ctaccatgcg gcgcagacgc
    65041 gaaatctgca cgtcgatgga gcgttccatt gcggaatatt cacgaccacg ggcaaggttc
    65101 atcagcttat cgcgggagag cggctcacgc ggatggctga ccagtgcctt cagtaccgca
    65161 aactcaccgc tggtgagcgg catcggctca tcttcgcgga acatttcgcg cgtaccgagg
    65221 ttaagtttga acttaccgaa agcaattacc gcctcttcct gtgacggtgc gcctggcagt
    65281 tcgttcgcct gacgacgcag caccgcacga atacgggcca gcagttcacg cgggttaaac
    65341 ggttttggaa tgtagtcgtc agcgccaatc tccaggccta cgatacggtc cacttcttcc
    65401 cctttcgccg tcaccataat gatcggcatc gggttgctct gactacgaag acgtcggcaa
    65461 atcgacaagc catcttcacc aggtaacatt aaatccagta ccataagatg gaaagattca
    65521 cgagtcagca ggcgatccat ctgttctgca ttagcgacgc ttcgaacctg gaagccttgt
    65581 tcggtgagat aacgttccag cagcgcacgc aggcgcatgt cgtcatcgac caccagaatc
    65641 ttgtagttct cttgcattgt ttgtactccc aaaggttcgc aacaatttgt aagcgtgtat
    65701 tcttaaaaaa gctcacgttc gtcaccagct aaatctggta tgaatttcag cctaaattgt
    65761 tacaaagcat attaaacagc agcttaagta tacaatttat tcggcgaaac attattgatt
    65821 ctgttgatat gatcacgtta tacccaatgt gcgcattatc aaacagacaa agggaatcaa
    65881 cgagatgaaa acgcccctgg ttacccggga agggtatgaa aaactcaaac aagagcttaa
    65941 ttatctctgg cgtgaagaac gcccggaggt cacaaaaaag gtgacctggg ccgcaagtct
    66001 gggcgaccgc agcgaaaatg ctgactatca gtataataaa aagcgtctgc gtgaaatcga
    66061 ccgtcgcgtg cgctatctca ctaaatgcct ggaaaatctc aaaattgtcg attactcccc
    66121 tcagcaggaa ggcaaagtct tttttggcgc gtgggtggag attgaaaacg acgatggcgt
    66181 gactcaccgt ttccgtattg tcggctacga tgaaattttt ggccgtaaag attacatctc
    66241 tatcgattcc ccgatggccc gcgcattgct gaaaaaagaa gtcggcgatc tggcggtggt
    66301 gaatacccct gccggggaag cgaactggta tgttaatgct atcgagtacg tgaaaccgta
    66361 aggaagagtc ttaacctcct gccgatggct ggcatttttg ccagccagtc cgtataacta
    66421 tcccctgatt tttgatccga aaagatgaac tcaaaccatg atgaatgatt cgttctgccg
    66481 cattattgcg ggtgaaattc aggcgcgccc ggaacaggtt gacgctgccg ttcgcctgct
    66541 tgatgaaggg aataccgtgc cgtttatcgc acgttatcgt aaggaaatca ccggcggtct
    66601 ggatgacacg cagctgcgta atctggaaac gcgtctgagc tatctgcgcg agctggaaga
    66661 gagacgtcag gcgatcctca aatccatttc cgagcaaggc aaactcaccg atgatctggc
    66721 gaaggccatc aacgccaccc taagcaaaac cgaactcgaa gacctctacc tgccctacaa
    66781 acctaaacgc cgcacccgcg ggcaaatcgc cattgaagca gggcttgagc cgttggctga
    66841 cctgctgtgg agcgatccgt cacacacgcc agaagtcgcc gctgcacaat atgttgatgc
    66901 cgataaaggc gtggcagata ccaaagccgc gctggatggc gcgcgctata tcctgatgga
    66961 acggtttgcc gaagatgccg cgctgctggc gaaagtgcgt gattatctgt ggaagaacgc
    67021 gcatttggtt tctacggtgg tgagcggtaa agaagaggaa ggggcgaaat tccgcgacta
    67081 tttcgatcat cacgaaccgt tgtccacggt gccttctcac cgcgcgctgg cgatgttccg
    67141 tgggcgtaac gaaggcgtac tccagctttc gctgaatgcc gatccgcagt tcgatgagcc
    67201 gcccaaagag agctattgcg agcaaatcat catggatcac cttggcctgc gcctgaacaa
    67261 tgccccggcg gatagctggc gcaaaagcgt agtgagctgg acctggcgca tcaaggtgct
    67321 gatgcatctg gaaaccgaac tgatgggtac cgtgcgcgaa cgcgcggaag atgaagcaat
    67381 caacgtcttt gcccgtaacc tgcacgatct gctgatggcg gcccctgccg gactgcgtgc
    67441 aacgatgggc ctcgatccgg gtctgcgtac cggggtaaaa gtggcggtgg tcgatgccac
    67501 tggcaaactg gtggcgaccg ataccattta cccgcacacc ggacaagccg caaaagcagc
    67561 gatgaccgtt gctgccttgt gtgaaaaaca taacgttgaa ctggtggcga tcggcaacgg
    67621 tacagcttcc cgcgaaactg aacgtttcta tctcgacgtg cagaagcagt tcccgaaagt
    67681 ggccgcacag aaagtgatcg tcagcgaagc tggcgcgtcg gtttactcag cttccgagct
    67741 ggcagcgcag gagttcccgg atctcgacgt ttcgctgcgt ggtgcggtat ctatcgcccg
    67801 ccgtttgcag gatccgctgg cggagctggt gaaaatcgat ccgaaatcta tcggcgtagg
    67861 tcagtatcag catgacgtca gccagacgca actggcccgc aaactggatg cagtagtaga
    67921 agactgcgta aacggcgttg gcgtcgatct caacaccgct tctgttccgc tattaacccg
    67981 cgtggcgggc ctgacgcgca tgatggcgca aaacatcgtg gcctggcgcg atgagaacgg
    68041 ccagttccag aaccgtcagc aactgctgaa agtcagccgt ctggggccga aagccttcga
    68101 gcagtgcgcg ggcttcctgc gcattaacca cggtgataac ccgctggacg cctctaccgt
    68161 tcatccggaa gcctatccgg tggtggaacg cattctggca gcaacacagc aggcactgaa
    68221 agatctgatg ggtaacagca gcgaactgcg taacctgaaa gcgtctgact ttactgatga
    68281 aaaattcggt gtgccgacag taactgacat catcaaagag ctggaaaaac cgggtcgcga
    68341 tccgcgtccg gaatttaaaa ccgctcagtt tgccgatggc gtcgagacaa tgaacgacct
    68401 gcaaccgggt atgatcctcg aaggcgcagt gaccaacgtc accaactttg gcgcgtttgt
    68461 cgatattggc gtgcatcagg acggcctggt tcacatctct tcattgtcga acaagtttgt
    68521 ggaagatccg cataccgtgg tgaaagcggg cgacattgtg aaggtgaaag tgctggaagt
    68581 ggatcttcag cgtaaacgta tcgccctgac tatgcgtctg gacgagcagc ctggcgaaac
    68641 caactctcgt cgcggcggcg gtaatgaacg cccgcaaaac aaccgcccgg cagccaaacc
    68701 acgcggtcgt gaagcgcagc ctgccggtaa tagcgcgatg atggatgcgc tggcggcagc
    68761 gatgggtaaa aaacgttaaa cgcccggact ggcccctaaa cctgcgcgat aaggcgttca
    68821 cgccgcatct ggcaacgaac gctttgcctg acataaaagt gccggagaat atctccggca
    68881 cttttattcc acagccaaac tcacaatata ctccgttaat atttatcact tcattaacaa
    68941 ctgaaacctt aattaaacat tagccagtcc gggtaactca ctattcgaat tatattttcg
    69001 ctgcgatata accttgagcc acatcaacat tgagtcagat tattattcaa accaacattc
    69061 gtacacattt taagtattgc tgatagaaac cattctcatt atcattgtgt tgttgattat
    69121 ttaatctctc ctttgttggc aaatcatctg gtctcatgtc gcggtcaaac gccccatgag
    69181 gtagttatcc agttaatgag aaacaagtag gcacctatgc aatacactcc agatactgcg
    69241 tggaaaatca ctggcttttc ccgtgaaatc agcccggcat atcgccaaaa actgctttct
    69301 ctaggcatgt tacctggctc ctcttttgat gtggtgcgcg tcgctccact cggcgacccc
    69361 attcatatcg aaacccgtcg tgtgagcctg gtattacgca aaaaagatct ggccttatta
    69421 gaagtggaag cggtttcctg ttaatacggt gataacaaca atgaaaaaat taaccattgg
    69481 cttaattggt aatccaaatt ctggcaagac aaccttattt aaccagctca ctggcgcacg
    69541 tcagcgtgta ggtaactggg ctggcgttac cgtcgaacgt aaagaagggc aattctccac
    69601 caccgatcat caggtcacgc tggtggacct gcccggcacc tattctctga ccaccatctc
    69661 atcgcagacc tcgctcgatg agcaaatcgc ctgtcactac attttgagtg gcgacgccga
    69721 cctgctgatt aacgtggtgg atgcgtctaa ccttgagcgt aacctgtacc tgacgctgca
    69781 actgctggaa ctcggcattc cctgcatcgt ggcgctgaac atgctcgaca ttgccgagaa
    69841 gcaaaatatt cgtattgaaa ttgatgctct atcggcacgt cttggctgcc cggtgatccc
    69901 gctggtttca acccgtggtc gcggtattga agcgctcaag ctggcgattg atcgctataa
    69961 agctaacgag aatgtggaac tggtgcatta cgcacagccg ctactcaacg aagcagattc
    70021 actggcaaaa gtgatgcctt ccgacatccc gctgaaacaa cgtcgctggc tgggcctgca
    70081 aatgctggaa ggcgatatct acagccgcgc ctacgccggt gaagcgtcgc agcatctgga
    70141 tgccaccctc gcccgtctgc gtaatgaaat ggatgatccg gcgctgcaca ttgccgatgc
    70201 gcgttaccag tgcattgctg ccatctgtga tgtggtaagc aacaccctga cggcagaacc
    70261 cagccgtttc accactgcgg tagataaaat cgtgctcaac cgtttcctcg gtctgccgat
    70321 tttcctcttt gtgatgtacc tgatgttcct gctggctatc aacatcggcg gggcgttaca
    70381 gccgctgttt gacgtcggct ccgtggcgct atttgtgcat ggtattcaat ggattggcta
    70441 cacgctccac ttcccggact ggctgactat cttcctcgcc cagggcctgg gtggcggtat
    70501 taacaccgtg ctgccactgg tgccgcagat cggcatgatg tacctgttcc tctccttcct
    70561 tgaggactcc ggctatatgg cgcgtgcagc gtttgtgatg gaccgcctga tgcaggcgct
    70621 gggcttgccg gggaaatcct ttgtgccgct gatcgtcggt ttcggttgta acgtaccgtc
    70681 ggtaatgggc gcacgtacgc ttgatgcacc gcgtgaacgt ctgatgacca tcatgatggc
    70741 accgtttatg tcctgcggcg cgcgtctggc tatcttcgca gtattcgcgg ctgccttctt
    70801 cgggcagaac ggtgccctgg cggttttctc gctgtatatg ctcggtatcg tgatggcggt
    70861 gctgactggc ctgatgctca agtacaccat catgcgcggt gaagcaacgc cgtttgtcat
    70921 ggagctgccg gtctaccatg taccacacgt taaaagcctg attatccaga cctggcagcg
    70981 tctgaagggc ttcgttctgc gtgctggtaa agtgattatc atcgtcagca ttttcctgag
    71041 cgctttcaac agcttctcgt tgagcgggaa aatcgtcgat aacatcaacg actcggcgct
    71101 ggcttccgtc agccgggtga taacccctgt cttcaagccg attggcgtac atgaagataa
    71161 ctggcaggca acggttggcc tgtttacggg cgcaatggca aaagaagtgg tggtgggtac
    71221 gctcaacacc ctctacaccg cagaaaatat tcaggacgaa gagttcaatc cggcggaatt
    71281 caacctcggt gaagagctgt tcagtgcggt agatgaaacc tggcagagcc tgaaagacac
    71341 cttcagcctt agcgtactga tgaaccccat tgaagctagc aaaggcgacg gtgaaatggg
    71401 taccggggcg atgggcgtga tggatcagaa attcggtagc gcagctgccg catacagcta
    71461 cctgattttc gtcctgctgt atgtaccatg tatctcggtg atgggagcca tcgcccgtga
    71521 atcaagccgt ggctggatgg gcttctccat cctgtggggg ctgaatatcg cttactcact
    71581 ggcaacattg ttctatcagg tcgccagcta cagtcagcac ccaacttaca gcctggtgtg
    71641 cattctggcg gtaatcctgt ttaacatcgt agttatcggt ctgctgcgcc gcgcgcgtag
    71701 ccgggtggat atcgaactgc tggcaacccg caagtcggta agcagttgct gcgcagccag
    71761 caccaccggt gattgccatt aatggcttca cttattcagg tacgtgattt gctggcgtta
    71821 cggtgccgta tggaagcgac ccagataagc cagacattga acactccgca gccaatgatt
    71881 aacgccatgc tgcaacagct ggaaagcatg ggcaaagccg tgcggattca ggaagaacct
    71941 gacggctgcc tctctggcag ttgtaaaagc tgcccggaag gcaaagcctg tctgcgcgag
    72001 tggtgggcgc tgcgttaacc atactccatc gcctgttttg aaaagcccgg tatgcgtttg
    72061 catccgggct tttttgcgtg cggctttcca taaaaatgca attcttgcag cgcaacgtaa
    72121 gttcctctga aagcgtctcg cagggatgaa aactcgctaa tgcacagatg tggagtggcg
    72181 cgtagagtcg cggcattcaa acaacaggtg aaggaacgcc atgagtaaaa agcagagttc
    72241 caccccacat gatgcgctgt tcaaactctt tttacgccaa ccggagacgg cacgcgattt
    72301 tcttgcgttt catttaccgg cacccattca cgcgctctgt gatatgaaaa ccctcaagct
    72361 ggagtcgagc agctttattg atgacgatct gcgtgaaagc tattccgatg tgctgtggtc
    72421 ggtgaaaacg gaacaaggac caggatacat ctattgtctg attgaacatc aaagcacctc
    72481 aaacaaactg atcgcatttc gcatgatgcg ttacgctatt gccgcaatgc aaaatcacct
    72541 tgatgctgga tacaaaacgt tgccgatggt ggtgccattg ttgttttacc acggtattga
    72601 aagcccctat ccctattcgc tgtgttggct ggattgtttc gccgatccca aactggcaag
    72661 gcagctttat gcctccgcat ttccgctgat tgatgtcacc gtcatgcctg atgatgaaat
    72721 catgcagcac cgacgtatgg cgctgctgga gttaattcaa aaacatattc gtcaacgcga
    72781 tctgatgggg ctggtagagc aaatggcctg cttattaagt agtggatacg ctaatgacag
    72841 acaaatcaaa gggctgttta attacatact gcaaactggc gacgctgtac gttttaacga
    72901 ttttatcgac ggcgttgccg aacgttcacc gaaacacaag gagagtttaa tgactattgc
    72961 ggaaagattg cggcaggagg gggaacaatc caaagccctg catatagcca aaataatgct
    73021 tgaatccgga gtccctcttg cagacatcat gcgatttacc gggctgtcag aagaagagtt
    73081 ggctgcggcg agtcagtaga tactctaccc tgcttaagtg ctactttgtg ctatcttgca
    73141 tgtaacacaa agtagcactt agggaactga tatgcagaca gtgaaattac ggcaacaagg
    73201 aggagccatg atcgttacta tcccacgaga tcttgccatc gatcttggct ggtcaccggg
    73261 cacagagctt accgtggaga aaaaaggcga tagcgttaat ttgcgcgcca cagagcacaa
    73321 accgcgtggg cgtcttaccg tcgcgcagtt gttgagccaa atcgatgaaa gcgaaattac
    73381 ggagctaaat cagtccacag aaggatgggc tgaagggaaa aaaggtaatg aagcatggta
    73441 aaggcacgga cgccacatcg tggtgagatc tggtatttta accctgatcc ggttgccggg
    73501 catgaacttc aggggccaca ttattgcatt gtggtaacgg acaaaaaact caacaatgtt
    73561 ttaaaagttg ctatgtgctg cccgatttca acaggggcaa atgcagcacg ttccacaggg
    73621 gtgacggtga acgtcctccc ctgtgatacg caaaccggta acctgcatgg cgttgtactt
    73681 tgtcaccagc taaaagccgt cgatcttatt gcccgtggcg ctaaatttca taccgttgcc
    73741 gatgaaaaat tgattagtga agttatcagt aaactggtga atttaatcga cccacaataa
    73801 ttagctctca cttaccacca acaccctctg cttcaacgcc accagcaggt gacaaaactc
    73861 gaccggatgc gaaataaacg gcgcatgggc cgctttggcg aagatatatg attcgctgtg
    73921 aggccaaagt ttatccagca tcggcaccac tttgcgcggc accagaccgt cgagatagcc
    73981 atacaatcgc aaaaacggca tggacacgtt ttgcagcggc tgacggagat cgaccgtttt
    74041 caggatttcc agcccgccat taagcacgtc aacctccggc atcggtaacg ccagaacggt
    74101 tttcttcaac gcccgcgcat cctggcgcgc cgtttcagtc cccatggttt gtaacgccag
    74161 gaaccgctcc actgtacgct gaaaatcatc actgagttgc tgctgaaatc ccgccagcac
    74221 gtccggtttt atccccggcc actcgtcacg agcactaaaa taaggtgacg acgccacggt
    74281 gaccagcgcc tgaacacgct cgggatgggt taacgcaatc tggcttgcca ccagcccgcc
    74341 cagactccag cctaaccaaa tggctttatc aggtgcctgt tgcagcacgg cttcggccat
    74401 atcagcaagt gacagcgcac caaatccccg gctacgcccg aagccgggca ggtcaacaag
    74461 gtgcagcgta aaatgcgagc taagttcctc gtcaatgcaa cgccacactt cggcattcag
    74521 tccccatccg tgcagcagca caagatgaac attcccctga cctttggtct gccaccagat
    74581 gttattcatc cgctattgtt ctcttttgac ttacaaggat gaacatatgc taacagtacc
    74641 gggattatgc tggctatgcc gaatgccact ggcgttaggt cattggggga tttgttcggt
    74701 ctgttcacgc gccacccgca cagataaaac gttatgccca caatgtggat tacccgccac
    74761 acactcccat cttccctgcg gtcgctgcct gcaaaaaccg ccgccctggc aaagactggt
    74821 cacggttgcc gactatgcgc cgccgttaag tccgcttatc caccagctta aattttcccg
    74881 gcgcagtgaa atcgccagcg ccctgtcacg tctgttacta ttggaagtct tacacgctcg
    74941 tcgcaccacc gggttgcaat tgccggatcg catcatcagc gttccgttat ggcagcggcg
    75001 tcactggcgt cggggattta atcagagcga tttgctgtgt cagccgttat cacgctggtt
    75061 gcactgccaa tgggatagcg aagccgtcac acgtacacgg gccactgcga cccagcattt
    75121 tctcagtgcc cggctgcgca agcgcaacct gaaaaatgcc tttcgtcttg aattgcccgt
    75181 gcaaggtcgc catatggtga ttgtggatga tgtcgttacc accggaagta ccgtcgcaga
    75241 gattgcgcag ttgcttttac gcaatggtgc ggcgactgtc caggtctggt gcctttgtcg
    75301 aaccttgtag agcctcgatg atgggcgtat tataaccaac taaaatagtc aactattagg
    75361 ccattactat gatccgtatt tccgatgctg cacaagcgca ctttgccaaa ctgctggcaa
    75421 atcaggaaga agggacacaa atccgcgtat ttgtgattaa ccctggcacg cctaacgctg
    75481 aatgtggtgt ttcttattgt ccgccggatg ccgtggaagc caccgacact gccctgaaat
    75541 ttgacctgct gaccgcgtat gttgatgagt taagcgcacc atacctggaa gatgcagaga
    75601 tcgactttgt taccgaccag ttgggttctc agttaacgct gaaagccccg aacgccaaaa
    75661 tgcgtaaagt ggcagacgat gcaccgctga tggagcgcgt ggagtatatg ctgcagtcgc
    75721 agatcaaccc acagcttgct ggtcacggtg gtcgcgtttc gctgatggaa atcaccgaag
    75781 acggttacgc cattctgcaa tttggcggcg gctgtaacgg ttgttccatg gtcgatgtga
    75841 cgctgaaaga agggatcgag aagcagctgc tgaacgaatt cccggagctg aaaggtgtgc
    75901 gcgatctcac cgaacaccag cgcggcgaac actcctacta ctaagttatc ccctcttttg
    75961 tagattgccc gatgcgacgc taaagcgtca tttcgggcct acgttgaaca tgtgccagac
    76021 gttggcacag cattgctttg gcaaatccct cccttcgttt ccccgcgata atatgaccaa
    76081 cctctcataa tttaaattta ccccgctctg gtgattctca aacgccagat gttacccgta
    76141 tcattcacat gggtaccaaa catactcctg acatctgact acaataatta gttttagtgg
    76201 gtatcagtcg tggtgccgca atatctctgt tcccgattgg gataattaga gtttgtcgtc
    76261 agaaaattga cgttacccat aacaaatgaa aggccaggta aatcatgcca ttagtcattg
    76321 ttgctatcgg tgtaatcttg ttgttgctcc tgatgatccg cttcaaaatg aacggcttca
    76381 tcgctctcgt cctcgtggcg cttgctgttg gattaatgca aggaatgccg ctggataaag
    76441 ttattggctc catcaaagcc ggtgtcggcg ggacgctcgg tagccttgcc ctgatcatgg
    76501 gttttggcgc aatgctggca aactgcggtg gcgcacaacg tatcgccacc acgctgattg
    76561 ccaaatttgg taaaaagcac atccagtggg cggtggtgtt aaccggtttt accgttggtt
    76621 ttgccctgtt ctatgaagtg ggcttcgtgc tgatgctgcc gctggtgttt accatcgcgg
    76681 cttccgcgaa tattccattg ctgtatgttg gtgtaccaat ggcggctgca ctgtctgtga
    76741 cccacggctt cctgccaccg catccgggcc cgactgcgat tgccaccatt ttcaatgccg
    76801 atatgggtaa aaccctgctg tacggtacta ttctggcaat cccgaccgtg attctcgccg
    76861 gtccggttta cgctcgcgtg ttgaaaggta tcgataagcc aatcccggaa ggtctctaca
    76921 gcgcgaaaac cttcagcgaa gaagagatgc cgagctttgg cgtcagcgtc tggacctctc
    76981 tggtgccggt tgtgctgatg gcgatgcgtg cgattgccga aatgatcctg ccgaaaggtc
    77041 acgctttcct gccggtagcg gagttcctcg gtgacccggt aatggcaacg ctgattgccg
    77101 tgctgattgc gatgttcacc tttggtctga accgtggtcg ttcaatggat cagattaacg
    77161 acacgctggt ttcttccatc aaaatcattg cgatgatgct gttgatcatc ggtggtggcg
    77221 gtgcgttcaa gcaggtgctg gtagacagcg gcgtggacaa atacattgct tccatgatgc
    77281 acgaaaccaa catttctccg ctgctgatgg cctggtcgat tgctgccgta ctgcgtatcg
    77341 cgctgggttc cgcaaccgtt gcggcaatca ctgcgggtgg tatcgcggca ccgctgattg
    77401 caacgacggg tgttagccca gagctgatgg ttattgcggt gggttccggt agtgtgattt
    77461 tctctcatgt gaacgatccg ggcttctggc tgttcaaaga gtactttaac ctgactatcg
    77521 gcgagaccat caaatcctgg tcgatgctgg aaaccattat ctcggtgtgc ggtctggtag
    77581 gctgtctgct gctgaatatg gtgatttgat aacactgact gccggatgca tcgcatccga
    77641 catcaaccgc actctacttc ttcttcgctg cagctctgcg ccgtctgtcc aaatccttca
    77701 gcaacttgtt cacgccatca tcggcaaaca tcgactcaag cgttgcggaa agcttgcgtc
    77761 gccagttttt atactggtaa ctggtgccag gaatattcac cggttcggcc atatccagcc
    77821 agtcttccgg ctgtagtcct aacagagcac tgttactgtc ggcaatgtag cgctgcaaac
    77881 cacggttcag cgtcggcgtc atcgacatca acgatgcctt atgcccggca cgttttggca
    77941 gacaaccata tttatgcagt gcatccagca gcccttgctt cgccagttcg cgatcctgat
    78001 acagaccgcg cagtaccact tcatccggat acagccccag ggttttgccc agcgttagat
    78061 ccccgctctc ccagtaacca cgcagcgttg gcaggtcatg tgtcgccgca accgccatcg
    78121 actgctccgg atacgctttc ggtgcacgga acgtcttctc gtggtcgttt tcgaaataga
    78181 gcactttgta agagtacaca ccgctgctgc gcagcttacc gacaatctct accggtacgg
    78241 taccgagatc ttcaccaatc accatacagc gatgacgttt actttcgagt gccagaatcg
    78301 agagcagatc atccaccgga tagtgaacat acgcgccctg atctgccgtc tcgccatacg
    78361 gtatccacca caaacgcagc atcgacatca catggtcaat tcgtaatgcg ccgcagtttt
    78421 gcatattggc acgcaacagc tcgataaacg gttcataggc acgcgcggtg atgatatgcg
    78481 ggtccattgg cggtaatccc cagttctgcc ccaacgggcc gaggatatcc ggcggcgcgc
    78541 caaccgatgc tttcaggcaa tatagttcac ggtcacacca ggtttccgcc ccaccttccg
    78601 ctacgccaac cgccagatca cgatacaagc caatcggcat ttcatagccc tggcttatct
    78661 cccagcaggc ggcaaactgg ctgtaagcca gccactgcaa ccagagataa aaatcgacgt
    78721 catcacgatg ttcttcgcag aactgacgca cttctggtga atccacgttc ttatacatct
    78781 ctggccatgc aggccagccc cagcgcattt cgtcctcttt cacttgctgg gcatgtagcg
    78841 catcaaaggc tgcctgccag aacaggctgt cgccctgctc tgcaacaaac tggcgaaacg
    78901 cggccatctg ctcatcatca cgttgcgcga aacctttcca cgccattcgt aatgctgtca
    78961 tttttagggc ggtaaccgtg gagtaatcga cccagtcggc atcgcgcgcc tgttgcagcg
    79021 tctgttgcgt ggtcggcaac tgccaccagg cctgagcctc ttcgctaaga tggaaatctt
    79081 caacggcgtt aacgtcgata taaatcacat tcagccaacg gcgagaagac gggctgtatg
    79141 ggctggcgct ctccggattt gccggataga gcgcatgaat cgggttcagg ccaatgaacg
    79201 acccgccacg ttttgccaca tccaccagca tcgctttgag atcgccaaaa tccccaatac
    79261 cccagttttt ttccgatcgc agcgtataaa gctgaacgca ggcaccccac agcttttgtt
    79321 tattcagcaa agcctgcggt tcgtaacaac gtttcggggc gacaatcacc cggcaatgcg
    79381 cgcgctggtc gtcctgggtg agtgttagcg tgtgataacc ttccggcagc ttcgtcggta
    79441 gattgaacgc tttgcccccc gttacatggc ctttgtactg cgttccttct tcggtggtca
    79501 gctgccagct atattcgccg ctgccctcca ccaccatcgg catttttttg ccgctggtat
    79561 aaaccatgac attcgggact ggcgttaccg ccactttcgt ggcggtacgt tgatgcatcg
    79621 cgtcaagcaa acgccgtttg gtttcggcgc taatcgactg cggtttaccg tgggcattga
    79681 tgtaattggg gctaatcccc gccgccagcg cggcattatc cagacgtttg ctttccatcg
    79741 agcttcctta gcgttttgcc tgccagatac gagcctgata atcgcgaata gagcgatccg
    79801 agctaaacat accgcagcgg gcggtattga ggatcgccgc gcgagtccag gcctcctggt
    79861 cgcggtacag cacatccacc tgcttttgtg cctcaacata ggctgcgaaa tccgccatca
    79921 ccagatacgg atcgccgccc tgtttgccga tactgtgcag catctggtcg aaggcatgct
    79981 tatcgccgtc gctgtattta ccgctttcca gctctttcaa tactgcgtcc agcaccttat
    80041 ctttcttccg ccatttcacc gggtcgtagc ctttggccag aatagccttc acttgttcca
    80101 cggtatgacc aaaaataaag atattttctt caccgacttt ctcggcgatt tcaacgttcg
    80161 caccatccag cgtaccgaca gtaagcgcac cattgagcgc cagtttcata ttgccggtac
    80221 cggaagcttc tttacctgca gtcgaaattt gttcggagat atccgccgcc gggatcagtt
    80281 tttccgccgc cgaaacgcaa taatccggca ggaacaccac cttcaactta tcgccaacca
    80341 gtggatcgtt gttgatcacg tcagccactt tgttgatcgc aaagataata ttcttcgcca
    80401 ggtagtagcc cggtgccgct ttcgcgccga agaggaagac gcgcggtacg cgatcagcct
    80461 gcaggttttc acgaatttct ttgtacaacg ccagaatatg cagcagattc aggtgctggc
    80521 gtttgtactc atgcagacgt ttgatctgaa tatcgaaaat cgcctgtgga ttgatctcaa
    80581 taccggtacg aactttcaca aactccgcca gacggacttt attcgcctgc ttgatctcgc
    80641 gatattgctg acggaatttc gcatcatcag cgaatttttc cagattgatc agctgatcga
    80701 gatcgttagc ccactctttt tgcagtgatt tatccaacag agccgccagt gcagggttgc
    80761 actgtttgat ccagcgacgt ggggtaatac cgttggtgac gttatggaat ttgttcggcc
    80821 atagctggtg atattccggg aacagatctt tcaccaccag atccgagtgc agcgccgcaa
    80881 caccgttcac cgcgaaaccg ccaaccacac acaggttcgc catatgcact tgtttatcgt
    80941 gcaccaccgc cagtttggcc cacacttttt catcgcccgg ccaggttttc tccaccagcg
    81001 ttttaaagcg agtattaatt tcgttaataa tctgcatatg gcgcggcagt aagcctttca
    81061 ccagtttcac atcccagcgt tccagcgctt ctggcatcag ggtatggttg gtgtaagcga
    81121 aagttttgct ggtgatggcc caggcgtcat cccagctcat ctggtgctca tcggtcagca
    81181 cgcgcagcag ttctggaatc gcgatagttg gatgggtatc gttcagctga ataacttcgt
    81241 aatccgccag ttcgtgcagt ttacgccccg ccagatgatg gcgacgcaaa atatccgcta
    81301 ccgaacaggc acactggaag tattgctgca tcaggcgcag ctttttaccg gcagtatggt
    81361 tgtcgtttgg atagagaact ttggtcagtt tttccgcatt gatgccttgc tgttcggcgc
    81421 gcaggaaatc accgtcgtta aatttagtta gatcaaacgg atgcgcatgc gtcgcctgcc
    81481 acagacgcag cggctgcgcc acgccattac gatagccgac aacggggaga tcccacgctt
    81541 gaccggtaat ggtaaactcc ggctcccagc gtccgtcttt catcacttta ccgccaatcc
    81601 ctacctgcac atccagtgct tcgttgtggc ggaaccacgg gtagttactg cgatgccagt
    81661 catccggcgc ttcaacctgt ttgccatcga caaaagactg gcggaacaaa ccatattgat
    81721 agttcagacc gtaacccgtc gcagactgac cgacagttgc cattgagtcg aggaagcacg
    81781 ccgccagacg tcccagacca ccgttaccca gcgccgggtc gatctcttct tccagcaggt
    81841 cagtcaggtt gatgtcataa gccttcaacg aatcctgtac atcctgatac cagccgagat
    81901 tcaacaggtt gttgcccgtc agacgaccaa tcaaaaactc cattgagatg tagttaacat
    81961 gtcgctgatt cgccaccggt ttggcgaatg gctgagcacg cagcatttcg gccagtgctt
    82021 cactcactgc cagccaccac tggcgaggag tcatttcagc cgcagaattt aagccataac
    82081 gctgccactg acgtgaaagc gcttcctgaa attgcttatc gttaaaaata ggttgtgaca
    82141 taggagttcc acttttctta gattttcaac acaacgttat cgctagtttg ccaggctcga
    82201 tgttgacctt cctcatcctg cgggggatta ggcagggagg agttgcgggg atgagcaagg
    82261 aaatgtgatc tcgaccactt aaagctagtg caaaccacag gattagcagc aaatcaatgc
    82321 catacagcgc agaaaatctg tatctaagtg caaaaaatgg ccgttgcgta ttttcaaaaa
    82381 gcggaaggta actctataaa ttaagtaaag gagtgaaaca gtttcacaag taaaatatcc
    82441 agtgtgctcc atctcattct taatagattt attaagatca tctttttaga tggcactttc
    82501 atcaggaatg aagaagaaac ccttgcttaa atgaatctga t
//
LOCUS       DADMEQ010000004        76407 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.004, whole genome shotgun sequence.
ACCESSION   DADMEQ010000004 DADMEQ010000000
VERSION     DADMEQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 76407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.004"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..545
                     /locus_tag="JNZ05_000267"
     CDS             <1..545
                     /locus_tag="JNZ05_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HAY8563629.1"
                     /translation="FPINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKEN
                     SLKRVINSFNSELMGEMSNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVI
                     EQRESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASY
                     TQLEIKHSHPKNRASGKRVS"
     gene            1088..1580
                     /locus_tag="JNZ05_000268"
                     /pseudo
     CDS             1088..1580
                     /locus_tag="JNZ05_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1683..2186
                     /locus_tag="JNZ05_000269"
     CDS             1683..2186
                     /locus_tag="JNZ05_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HAY8563630.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLDEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(2253..3710)
                     /gene="nuoN"
                     /locus_tag="JNZ05_000270"
     CDS             complement(2253..3710)
                     /gene="nuoN"
                     /locus_tag="JNZ05_000270"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HAY8563631.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3717..5246)
                     /gene="nuoM"
                     /locus_tag="JNZ05_000271"
     CDS             complement(3717..5246)
                     /gene="nuoM"
                     /locus_tag="JNZ05_000271"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HAY8563632.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITVATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(5410..7251)
                     /gene="nuoL"
                     /locus_tag="JNZ05_000272"
     CDS             complement(5410..7251)
                     /gene="nuoL"
                     /locus_tag="JNZ05_000272"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HAY8563633.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(7248..7550)
                     /gene="nuoK"
                     /locus_tag="JNZ05_000273"
     CDS             complement(7248..7550)
                     /gene="nuoK"
                     /locus_tag="JNZ05_000273"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HAY8563634.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7547..8101)
                     /gene="nuoJ"
                     /locus_tag="JNZ05_000274"
     CDS             complement(7547..8101)
                     /gene="nuoJ"
                     /locus_tag="JNZ05_000274"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HAY8563635.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(8113..8655)
                     /gene="nuoI"
                     /locus_tag="JNZ05_000275"
     CDS             complement(8113..8655)
                     /gene="nuoI"
                     /locus_tag="JNZ05_000275"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HAY8563636.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8670..9647)
                     /gene="nuoH"
                     /locus_tag="JNZ05_000276"
     CDS             complement(8670..9647)
                     /gene="nuoH"
                     /locus_tag="JNZ05_000276"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HAY8563637.1"
                     /translation="MSWISPELIEILLTILKAVGILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9644..12370)
                     /gene="nuoG"
                     /locus_tag="JNZ05_000277"
     CDS             complement(9644..12370)
                     /gene="nuoG"
                     /locus_tag="JNZ05_000277"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HAY8563638.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(12423..13760)
                     /gene="nuoF"
                     /locus_tag="JNZ05_000278"
     CDS             complement(12423..13760)
                     /gene="nuoF"
                     /locus_tag="JNZ05_000278"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HAY8563639.1"
                     /translation="MKNITRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13757..14257)
                     /gene="nuoE"
                     /locus_tag="JNZ05_000279"
     CDS             complement(13757..14257)
                     /gene="nuoE"
                     /locus_tag="JNZ05_000279"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HAY8563640.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(14260..16062)
                     /gene="nuoC"
                     /locus_tag="JNZ05_000280"
     CDS             complement(14260..16062)
                     /gene="nuoC"
                     /locus_tag="JNZ05_000280"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HAY8563641.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRTQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(16156..16818)
                     /locus_tag="JNZ05_000281"
     CDS             complement(16156..16818)
                     /locus_tag="JNZ05_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HAY8563642.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16834..17277)
                     /gene="nuoA"
                     /locus_tag="JNZ05_000282"
     CDS             complement(16834..17277)
                     /gene="nuoA"
                     /locus_tag="JNZ05_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HAY8563643.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRECMNPETNSIANRQR"
     gene            complement(17908..18846)
                     /gene="lrhA"
                     /locus_tag="JNZ05_000283"
     CDS             complement(17908..18846)
                     /gene="lrhA"
                     /locus_tag="JNZ05_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HAY8563644.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19771..20988
                     /locus_tag="JNZ05_000284"
     CDS             19771..20988
                     /locus_tag="JNZ05_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAY8563645.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            21072..21671
                     /gene="yfbR"
                     /locus_tag="JNZ05_000285"
     CDS             21072..21671
                     /gene="yfbR"
                     /locus_tag="JNZ05_000285"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY8563646.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21730..23562)
                     /locus_tag="JNZ05_000286"
     CDS             complement(21730..23562)
                     /locus_tag="JNZ05_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HAY8563647.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23649..24299)
                     /gene="hxpA"
                     /locus_tag="JNZ05_000287"
     CDS             complement(23649..24299)
                     /gene="hxpA"
                     /locus_tag="JNZ05_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HpxA"
                     /protein_id="HAY8563648.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(24310..24804)
                     /locus_tag="JNZ05_000288"
     CDS             complement(24310..24804)
                     /locus_tag="JNZ05_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAY8563649.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24887..25342)
                     /locus_tag="JNZ05_000289"
     CDS             complement(24887..25342)
                     /locus_tag="JNZ05_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HAY8563650.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25680..26882
                     /gene="ackA"
                     /locus_tag="JNZ05_000290"
     CDS             25680..26882
                     /gene="ackA"
                     /locus_tag="JNZ05_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAY8563651.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26957..29101
                     /gene="pta"
                     /locus_tag="JNZ05_000291"
     CDS             26957..29101
                     /gene="pta"
                     /locus_tag="JNZ05_000291"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY8563652.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            29291..30811
                     /gene="yfcC"
                     /locus_tag="JNZ05_000292"
     CDS             29291..30811
                     /gene="yfcC"
                     /locus_tag="JNZ05_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAY8563653.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30844..31386)
                     /gene="yfcD"
                     /locus_tag="JNZ05_000293"
     CDS             complement(30844..31386)
                     /gene="yfcD"
                     /locus_tag="JNZ05_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HAY8563654.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(31444..31995)
                     /gene="yfcE"
                     /locus_tag="JNZ05_000294"
     CDS             complement(31444..31995)
                     /gene="yfcE"
                     /locus_tag="JNZ05_000294"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HAY8563655.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(32051..32695)
                     /gene="yfcF"
                     /locus_tag="JNZ05_000295"
     CDS             complement(32051..32695)
                     /gene="yfcF"
                     /locus_tag="JNZ05_000295"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HAY8563656.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32831..33478
                     /gene="yfcG"
                     /locus_tag="JNZ05_000296"
     CDS             32831..33478
                     /gene="yfcG"
                     /locus_tag="JNZ05_000296"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HAY8563657.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33535..33897
                     /gene="folX"
                     /locus_tag="JNZ05_000297"
     CDS             33535..33897
                     /gene="folX"
                     /locus_tag="JNZ05_000297"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HAY8563658.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIVREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33918..34811
                     /locus_tag="JNZ05_000298"
     CDS             33918..34811
                     /locus_tag="JNZ05_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HAY8563659.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSHWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34859..35749)
                     /locus_tag="JNZ05_000299"
     CDS             complement(34859..35749)
                     /locus_tag="JNZ05_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY8563660.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35946..36719)
                     /gene="hisP"
                     /locus_tag="JNZ05_000300"
     CDS             complement(35946..36719)
                     /gene="hisP"
                     /locus_tag="JNZ05_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HAY8563661.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36727..37443)
                     /locus_tag="JNZ05_000301"
     CDS             complement(36727..37443)
                     /locus_tag="JNZ05_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8563662.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(37440..38126)
                     /locus_tag="JNZ05_000302"
     CDS             complement(37440..38126)
                     /locus_tag="JNZ05_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708190.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY8563663.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     LGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(38216..38998)
                     /gene="hisJ"
                     /locus_tag="JNZ05_000303"
     CDS             complement(38216..38998)
                     /gene="hisJ"
                     /locus_tag="JNZ05_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HAY8563664.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(39219..40001)
                     /gene="argT"
                     /locus_tag="JNZ05_000304"
     CDS             complement(39219..40001)
                     /gene="argT"
                     /locus_tag="JNZ05_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HAY8563665.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALLPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(40267..40836)
                     /locus_tag="JNZ05_000305"
     CDS             complement(40267..40836)
                     /locus_tag="JNZ05_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY8563666.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40931..42448)
                     /gene="purF"
                     /locus_tag="JNZ05_000306"
     CDS             complement(40931..42448)
                     /gene="purF"
                     /locus_tag="JNZ05_000306"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAY8563667.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(42485..42973)
                     /gene="cvpA"
                     /locus_tag="JNZ05_000307"
     CDS             complement(42485..42973)
                     /gene="cvpA"
                     /locus_tag="JNZ05_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HAY8563668.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(43232..43894)
                     /gene="dedD"
                     /locus_tag="JNZ05_000308"
     CDS             complement(43232..43894)
                     /gene="dedD"
                     /locus_tag="JNZ05_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HAY8563669.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43884..45152)
                     /gene="folC"
                     /locus_tag="JNZ05_000309"
     CDS             complement(43884..45152)
                     /gene="folC"
                     /locus_tag="JNZ05_000309"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAY8563670.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIELGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGRELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADAAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(45222..46136)
                     /gene="accD"
                     /locus_tag="JNZ05_000310"
     CDS             complement(45222..46136)
                     /gene="accD"
                     /locus_tag="JNZ05_000310"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HAY8563671.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(46292..46951)
                     /locus_tag="JNZ05_000311"
     CDS             complement(46292..46951)
                     /locus_tag="JNZ05_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8563672.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(47034..47846)
                     /gene="truA"
                     /locus_tag="JNZ05_000312"
     CDS             complement(47034..47846)
                     /gene="truA"
                     /locus_tag="JNZ05_000312"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAY8563673.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47846..48859)
                     /locus_tag="JNZ05_000313"
     CDS             complement(47846..48859)
                     /locus_tag="JNZ05_000313"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY8563674.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQLWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48925..50061)
                     /gene="pdxB"
                     /locus_tag="JNZ05_000314"
     CDS             complement(48925..50061)
                     /gene="pdxB"
                     /locus_tag="JNZ05_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HAY8563675.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            50160..51155
                     /locus_tag="JNZ05_000315"
     CDS             50160..51155
                     /locus_tag="JNZ05_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HAY8563676.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMPTRPALWILLVAIILMLVWLVR"
     gene            complement(51152..52330)
                     /locus_tag="JNZ05_000316"
     CDS             complement(51152..52330)
                     /locus_tag="JNZ05_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HAY8563677.1"
                     /translation="MTAVSQTETRSSASFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52605..53825)
                     /gene="fabB"
                     /locus_tag="JNZ05_000317"
     CDS             complement(52605..53825)
                     /gene="fabB"
                     /locus_tag="JNZ05_000317"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HAY8563678.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDITGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53984..55990
                     /gene="mnmC"
                     /locus_tag="JNZ05_000318"
     CDS             53984..55990
                     /gene="mnmC"
                     /locus_tag="JNZ05_000318"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HAY8563679.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAR"
     gene            complement(56111..56389)
                     /locus_tag="JNZ05_000319"
     CDS             complement(56111..56389)
                     /locus_tag="JNZ05_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HAY8563680.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(56423..56971)
                     /locus_tag="JNZ05_000320"
     CDS             complement(56423..56971)
                     /locus_tag="JNZ05_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HAY8563681.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56971..57780)
                     /locus_tag="JNZ05_000321"
     CDS             complement(56971..57780)
                     /locus_tag="JNZ05_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAY8563682.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57780..58604)
                     /gene="mepA"
                     /locus_tag="JNZ05_000322"
     CDS             complement(57780..58604)
                     /gene="mepA"
                     /locus_tag="JNZ05_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HAY8563683.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58608..59693)
                     /gene="aroC"
                     /locus_tag="JNZ05_000323"
     CDS             complement(58608..59693)
                     /gene="aroC"
                     /locus_tag="JNZ05_000323"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAY8563684.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKEGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59728..60660)
                     /gene="prmB"
                     /locus_tag="JNZ05_000324"
     CDS             complement(59728..60660)
                     /gene="prmB"
                     /locus_tag="JNZ05_000324"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY8563685.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            60826..61377
                     /gene="smrB"
                     /locus_tag="JNZ05_000325"
     CDS             60826..61377
                     /gene="smrB"
                     /locus_tag="JNZ05_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HAY8563686.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(61481..62371)
                     /locus_tag="JNZ05_000326"
     CDS             complement(61481..62371)
                     /locus_tag="JNZ05_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HAY8563687.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(62358..62882)
                     /locus_tag="JNZ05_000327"
     CDS             complement(62358..62882)
                     /locus_tag="JNZ05_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8563688.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62879..63349)
                     /locus_tag="JNZ05_000328"
     CDS             complement(62879..63349)
                     /locus_tag="JNZ05_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8563689.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDIAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(63346..63894)
                     /locus_tag="JNZ05_000329"
     CDS             complement(63346..63894)
                     /locus_tag="JNZ05_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8563690.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63899..64621)
                     /locus_tag="JNZ05_000330"
                     /pseudo
     CDS             complement(63899..64621)
                     /locus_tag="JNZ05_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64641..67282)
                     /gene="yfcU"
                     /locus_tag="JNZ05_000331"
                     /pseudo
     CDS             complement(64641..67282)
                     /gene="yfcU"
                     /locus_tag="JNZ05_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(67364..67927)
                     /locus_tag="JNZ05_000332"
     CDS             complement(67364..67927)
                     /locus_tag="JNZ05_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY8563691.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(68611..69096)
                     /gene="sixA"
                     /locus_tag="JNZ05_000333"
     CDS             complement(68611..69096)
                     /gene="sixA"
                     /locus_tag="JNZ05_000333"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HAY8563692.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(69299..71443)
                     /gene="fadJ"
                     /locus_tag="JNZ05_000334"
     CDS             complement(69299..71443)
                     /gene="fadJ"
                     /locus_tag="JNZ05_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HAY8563693.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(71443..72753)
                     /gene="fadI"
                     /locus_tag="JNZ05_000335"
     CDS             complement(71443..72753)
                     /gene="fadI"
                     /locus_tag="JNZ05_000335"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HAY8563694.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(72933..73217)
                     /locus_tag="JNZ05_000336"
     CDS             complement(72933..73217)
                     /locus_tag="JNZ05_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HAY8563695.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            73589..74929
                     /gene="fadL"
                     /locus_tag="JNZ05_000337"
     CDS             73589..74929
                     /gene="fadL"
                     /locus_tag="JNZ05_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HAY8563696.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLQADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            75295..76346
                     /locus_tag="JNZ05_000338"
                     /pseudo
     CDS             75295..76346
                     /locus_tag="JNZ05_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        19318 a        20451 c        18740 g        17898 t
ORIGIN      
        1 tattccccat aaatactggc ggacactgga tattagtttc gctacagaaa atagtaaata
       61 aaaaaaataa taaactacaa ataaaatgcg tcatattcaa ctcattgcgt gcactaggcc
      121 atgataaaga aaattcactt aagcgtgtca ttaatagttt taattctgaa ctcatgggag
      181 aaatgtcgaa taataatata aaagttcatt taaatgaacc agagataata tttttacatg
      241 ccgatcttca gcaatactta agccaaagtt gcggtgcgtt tgtgtgcatg gcagcccagg
      301 aagtgattga acaaagggaa agcaattctg acagcgcccc ctatacgtta ttaaaaaact
      361 atgctgacag atttaaaaaa tattcagcag aagagcagta cgaaattgat tttcaacatc
      421 gactggtaaa cagaaattgt tatttagata aatatggcga tgcacgaatc aatgcttctt
      481 atacacaatt agaaataaaa cactcacacc ccaaaaatag agcatccggc aaaagagtga
      541 gttaacatca ttgttaatca attcgaccag tcagtaaata tgctgactgg tcgaacataa
      601 tttgctaaaa agtcatcact gactgatgtc agtcccacca tctgccagtt caatctacgg
      661 gtatttccag cgtatcgcgg cacgcgattg ctggtctgtt gatgtagtgg attgtagttg
      721 ctgacttttc ttaccctcag atttctgccc cacttgtcaa ttcaatctgc caataaataa
      781 cataattata tttttattcc tcacagaaat tcctttttaa aaatagcgaa cccactgtaa
      841 aaaacacttt ttgtatacct ctaccaagcg taataaaata aacgctcata attcattata
      901 aataatgatg aaataacgcc ttttattgat aatttacata tgaaaaaatt tttccttttg
      961 ttatattatt tctgttttca ctccctctga ttatttttac caaccctggg tcactgcatt
     1021 atcaccaacg ccccgacacg taagtcctga gcaattagaa aaaacagttc gttatcttac
     1081 acaaactgtg catccacgta atgccggcaa tattgataat ctgaataggt ccgcagagta
     1141 tataaaagag ttctttgtca gtagcggtgc cagagttacc gcgcagggcg tccccgattg
     1201 gctattacga ctctgcgcct ggaagccaga attaccctta cccggcaatg tcctggcttt
     1261 atcccgatcg gggagacttt attgctgtgg tcggcagaat gcaggatatc aacgccgttc
     1321 gtcaggtaaa agcggcattg ttgtcatccc gggatttatc tgtttattct atgaatgccc
     1381 cagggtttat tcccggtatt gatttttctg atcaccttaa ttattggcaa cacgatattc
     1441 ccgccgtaat gattactgac accgcttttt atcgtaataa acaataccac ttgcccggtg
     1501 ataccgcaga cagattgaat tatcagaaaa tggctcaggt ggtagatggt gttataacct
     1561 tgttatacaa cagtaaataa taaaaatttc tcgaggcggg tccagaaaca gcgacatgta
     1621 cattttattt tccctcgtct gttttaagat gcgaaacata aaattcaatg gaggaaaaat
     1681 aaatgggaat gattggctat tttgcagaaa ttgactcaga aaagattaac caactgcttg
     1741 agtctactga aaaacctttg atggataata tacatgacac gctttcagga ttacgccgac
     1801 tggatattga taaacgctgg gattttttac actttgggct gacaggcacc tctgcttttg
     1861 atcccgcgaa gaatgatccg ctgagtcgtg ccgtgcttga tgaacacagc ctcgaagatg
     1921 gcatagatgg cttcctggga ttgacctgga atcaggaatt agccgcgaca atcgaccgac
     1981 tggaatcact ggatcgaagt gagctgcgta aacagttttc cattaagcga ctcaatgaaa
     2041 tggagattta tccgggagtg accttttcag aagagttaga ggggcaactc tttgcctcga
     2101 tcatgcttga tatggaaaag ctgattagcg cgtatcgacg tatgctgcga caagggaatc
     2161 atgcgctgac ggttattgtt gggtgatggt gtcagagcgc caaaaaaaac cgccgaacat
     2221 gtcggcggct ttctgactta caaactaaca gtttacatca gcggcattgc taaccgcaca
     2281 atactaatca gcggttgtgg ccatacaccc agcaccagta ccaacagtgc agaaatcagc
     2341 accacgatac cgcccgcgct gtactgccag tttgacggtg catcgcgacc cggttgttcc
     2401 ggtgcgtgca ggtacaggct caccgccacg cgcaggtagt agtagaggcc gattgccgaa
     2461 ccgacaacca cggcacccac cagccaccac aagtgtgcct ggacaccgac cgccagcacg
     2521 tagaacttac cgataaagcc cagcgtcatc gggataccgg ccagagacag catcatcacc
     2581 gtcatcactg ccgcgaggat cggacgatgc cagaacagac cgcggtaaga gaacagggaa
     2641 tcagcatccg ggccacgata cgggctggac atcaggctga ccacgccgaa cgcgccgagg
     2701 ctgctgaaca gataaccggc caggtaaacc cctaccgctt ccatcgacat ctcgccggtt
     2761 tgcagcgcaa tcagcgccac cagcagatag ccgaggtgag agatagatga gtaaccgagc
     2821 agacgtttga tgttggtctg gctcaacgcc atcaggttac cgaagatgat ggaggcaaag
     2881 gcgataatcg ccagtactac gcgaatcgct tcgctgtcac ccaccggtgc gtagaggaac
     2941 agacgcatca ccacaccgaa gatagcgatt ttgctcgccg tcgccaggaa agtggaaacc
     3001 ggcgcaggcg cgccctggta tacgtctggc gtccacaggt ggaacggcac cagagagagt
     3061 ttgaagccga ggccaacaat catcatgccg aaacctgcca gcaacagcgg ctcgttgagc
     3121 ataccgtcgc cgaggttttt acccagcgcg acaaacgaca ggtcgccaga ctgcgcatac
     3181 accagcgcca taccgaacag caggaaagaa gacgctgcgg cagaaaggat ggtgtatttg
     3241 atactggctt ccagcgaacg tttctggcgg aaagcgtaac cgaccaggcc aaacagcggc
     3301 aaagagatca gttcaatacc gaggaacaga gacgccagat ggtttgcatt cgccagcagg
     3361 atcccgccca gcgcggcaat taacaccaac aggtagaact catccttgtt gtcgttatag
     3421 ccttcaagcc acgggtaggc gaaagtacag gtggcgaggc tcgccaacaa taccagcccg
     3481 gtgtaaagca tggcgaaacc atcaacgcgc atcagcggcg taacgtccat agcgcccgcc
     3541 tggccaacaa accagagcga aaccagcgcc gcgttaagcc caataaccga gagcgtagcg
     3601 ttgaggaaat gattgcgtcg ccacgcaatg gagagcatca caaccaccac cgtcaagccg
     3661 acgatcagca acggtagcag tgcgatcagg ttttgtggag ttattgtcat ggcgatttac
     3721 ggccttgtag tagtaacgga attaacaaac cactgctgga tattgccaat cgcggagtgc
     3781 gaggtatcca gaatcggctg cggatagaag cccagcagta ccagcagcac caccagcaac
     3841 aggatcataa acagctcacg cagcgacatc cctggcagtt cctggctggc aatctggctt
     3901 ttcgctttac cgaagtaagc gcgatgcaac atcgccagcg aataaacaga tgcaaagacc
     3961 agcccaaagg tagagataac ggtaatcacc gggacaacct ggaagctgcc gaacagaatc
     4021 ataaattcgc cgacgaagtt accggtgcca ggcatcccaa gcgttgccac cgcaaagaac
     4081 agcgacagtg ctggcagcca tttcatcttg ctccacagac cgcccatcat gcgcatgtcg
     4141 cgggtatgga tacgttcata aagctgaccg cagagaataa acagacccgc cgccgacaag
     4201 ccgtgcgcaa tcatctggat taccgcgccc tggtaggcca actggctgcc ggtgtagatt
     4261 gcaatcagca cgaagcccat gtgggaaacc gaggtgtagg cgatcagacg tttgatatcg
     4321 gtctgagcga aggccatcca cgcaccgtag aagatgccga taacacccag ccacatagcg
     4381 attggcgcga actctgccga cgcgttcggg aacagcggca gggagaaacg caacaaaccg
     4441 taagcggcgg ttttcagcaa gatccccgcg aggtccacgg aaccggcggt cggagcctgg
     4501 gagtgcgcat ccggcagcca gccatgcagc ggaaccaccg gcattttgac tgcgaaggcg
     4561 atgaagaagc ccagcatcaa cagatattcc acaccactgg acattggcgt attcagcagc
     4621 tcttcatagt tgaaggtcca gacgccggtc gcattgtagt gaacaaaaac cagcgccagg
     4681 atggcgatca acatcaccag accactcgcc tgggtgtaaa tgaagaactt ggttgccacc
     4741 gtgatacgcg ttttaccgtc agaggcttta tgcccccaca gtgcgatcag gaagtacatc
     4801 ggcaccagca tcatttccca gaagaagaag aacaggaaca tgtcgatggc aaggaacacg
     4861 ccgataacgc cgcccaggat ccacatcagg ttgaggtgga agaagccctg atatttttcg
     4921 atctctttcc acgaacatag taccgccagc acaccgagca gaccggtcag cacgaccatc
     4981 agcagcgaca gaccgtcaat ggcgagatga atagagatac caaaacgcgg gatccacggc
     5041 atgtcgaatt cagactgcca ctgcggaatt ccggcggatt gcgtcagtga ataaccgccc
     5101 tgcaaccaca gttgcagcga cagcgccagc gtcaatccca tggtgatcag cgcgatccag
     5161 cgtggcacct tgacgccaaa gcgttcggtc tgccagcaca ggaagccgcc aataaaggga
     5221 attaatatta gccagggtag taacatggcg atctttattc cttgtaaaag tcccgtcagg
     5281 accggatttt caacgaattc tcacgacaga aaattctctt cggactgggg gtagtgtcgg
     5341 atgcgccgcg aaggcagctt atccgaccta cgttcttata tgcccagggg caatcccaca
     5401 atccttaact caacgcagta ccatcaacag tgccagcacc acgaccgcac cgatgctcat
     5461 ggatgccaca taccagcgca gataaccgtt ctcgcttaac agcagacctt tacctgcaaa
     5521 gcgggaaaga accgccggga tgttcatcat tgagttcagc ggatcgcgtt tcagcaacca
     5581 ggcaataccc aggaacggct tgacgaacac tttgtcgtac agccagtcaa agccccaggc
     5641 gttgtaccac caggtactca gcaggcggcc cggcgcactg ttggcgatgg aggtcaccag
     5701 agtacgttta cccagccaca gccaggctgc cagcagaatg ccgaccaccg cgaccacgcc
     5761 agaggtaatt tccagggtca acatgctgcc gtgcgccagt tccgtcgttt gcggaagcac
     5821 gccctgcagc ggcggtacaa tcagtgcgcc aacgaaggtg gaaaggatca gcagcacaat
     5881 cagcggcagg ctgtgagtta cccctttcac ggcgtgagcg tgaatttgtt cttttccgtg
     5941 gaagacgatg aaaatcatac ggaaggtgta gagcgaggtc ataaacgcac cgaccagacc
     6001 tgccaccatc agattgatat gaccattcgc catcgcaccc gcgaggatct catccttact
     6061 gaagaagccc gcagtgacca gcggcagtgc cgacagtgct gcgccgccca ccaggaagca
     6121 gagataaacc agcggaatag atttacgcag accgcccatc ttgaagatgt tctgttcgtg
     6181 atggcaggcc agaatgacgg aaccggatgc caggaacagc agcgctttaa agaacgcgtg
     6241 agtcatcaag tggaaaatcg ccgcatccca tgcctgcacg ccaagcgcga ggaacatgta
     6301 gccaatctgg ctcatggtag agtaagcgag aacacgtttg atgtcggtct gtaccagcgc
     6361 ggcaaaaccg gccagcagca gcgtaaccgc cccgacaata cccaccagat gcagaacttc
     6421 cggcgtcatc aggaacaggc cgtgggtacg ggcgatcagg tagacacccg cggttaccat
     6481 cgttgcggcg tggatcagcg cggagacagg cgtcgggccc gccatcgcgt cagcaagcca
     6541 tgtctgcaac ggcaactgcg cagatttacc gaccgcaccg cccagcagca tcagcgtcgc
     6601 ccacatcagc atgttattgc cgtcagcaaa gtgcgctggt gccagttcca ccatttcgcg
     6661 gaagttcagg gtgcccagtt cgttgtaaag aatgaacagt gcgaaagcga ggaaaacgtc
     6721 acccacacgg gtcacgacga acgctttcat tgctgctgcg ccattcttcg gatcggtgta
     6781 atagaaaccg atcagcagat aggagcacag gcccacgcct tcccagccga ggtacatcag
     6841 cagcaggtta tcggcgagaa ccagaactac catgctggcg atgaacaggt tggtgtaggc
     6901 gaagaagcga gagtagccct cttcaccgcg catgtaccag gaggcgtaca tgtgaataag
     6961 gaaacccaca ccagtgacca ccgagagcat ggtcagcgac aggccgtcca gcaccaggtt
     7021 aaaaccgatg ttaaagtcgc ctaccgacat ccacgtccac agcggctggc tgtatgcctg
     7081 ctcgccgtta gcgaagaaat caacgccgat aaaggcggtt accagcgccg ccaggcccac
     7141 agagccaacg ccgacgatcg ccgaaacgtt ttcagaccag cgcccacggg agaacgccag
     7201 caggacgaag ccaatcaatg gcaaaataat ggttaaggca agcatgttca tccgcgcatc
     7261 tcacttactg aatcgatgtt caggttctgg cgacgacggt gaagttgcag cagcagcgca
     7321 aggccgatac tcgcttctgc cgccgcgagg ctgatggcga gaatgtacat cacctgaccg
     7381 tcggtctgac cccagtagct tccggcgacc acaaaggcca gcgcggaggc gttaatcatg
     7441 atttccagac caatcagcat aaacagcaga ttgcgacgga taaccagacc ggttaagcca
     7501 agaacgaata agattgccgc gaggatcagt ccatgttgta aggggatcat gcgtgctcct
     7561 ccgtttttct tttcgcgctg tcgtctttac gattgctcag cacttcaccc gcacgctctt
     7621 cacgaccgac gtggaaggcc acaaccagac ccgcgagcag cagcatagaa gccagttcca
     7681 ccgccagtac gtaaggcccg aacagcgtaa tacccactgc tttagcactg attggcgtac
     7741 cgtcgatacc ctgatcgtta acaccgagga tggcgtaaac aatcaccacc agcatgatgg
     7801 ccgacaaaat tgccgggcca atccacacct gcggtttcag ccactggcgt tcctgttcga
     7861 tttctgaacc gcccaggttg agcatcatca ccacgaacac gaacagcacc ataatggcac
     7921 ccgcgtagac gataatttcc agcgcaccgg cgaagtaagc gcctagtgag aagaacaccc
     7981 cggaaatcgc cagcagcgaa ataatcaggt acagcagtgc gtgtaccgga ttggtatggg
     8041 tgatcactcg caaggtcgca agtatggcta tcaggccaca gatataaaaa gcgaactcca
     8101 ttgcccctct ccttacggta acaggctctt gacgtcgata ggcttggctt cgttctctgc
     8161 ttcgccctta tctttgccgt cgattgccat acctgccatc cggtagaagt tatattccgg
     8221 gtatttgccc ggaccggaga tcagcagatc ctctttctcg taaaccagat cctggcgctt
     8281 gtattccccc atttcgaaat ccggggttaa ctgaatcgcc gtggtcggac aggcttcttc
     8341 gcacagacca cagaaaatgc agcgtgagaa gttaatgcgg aaaaattccg gataccagcg
     8401 accgtctttg gtttctgctt tttgcagcga gatacagccg accgggcagg ctaccgcaca
     8461 gaggttacag gctacgcaac gctcttcgcc gtccgggtcg cgggtcagaa cgatacgacc
     8521 acgataacgg ggcggcagat agaccggctc ttccgggtac attcgcgttt cgcgtttggc
     8581 gaacgcgtgc aggccgatca tccagatact acgaacctgg gtgccgaaac ctactaacaa
     8641 ttcttttaag gtcatggtct tattgcccct tattgcgcct gccagagaat gacagccgcc
     8701 gttaccagca agttgatcag cgtcagcggc aggcagattt tccagccgaa ggacattacc
     8761 tggtcataac gcggacgcgg taacgacgca cgaatcaaaa tgaacatcat cataaagaac
     8821 gcggttttca gcgcgaacca gatgaatggc ggtaacaacg ggccttgcca gccaccgaag
     8881 aacagcgtca ccatcaatgc agagatggtc acaatcccga tgtactcacc cacgaagaac
     8941 agaccgaact tcataccgga atattcaatg tggtaaccat ccgccagttc ctgctcggct
     9001 tccggctggt caaacgggtg acggtgacac accgccacgc ccgcgatggc aaaggtaata
     9061 aaaccaaaga attgcgggat gacgttccac acatgcgcct ggctgttgac gatgtcggtc
     9121 atgttgaatg aaccggcctg cgccaccacg cccatcaggg aaagcccgag gaacacttcg
     9181 tagctcaggg tctgcgcaga agcacgcatc gcacccagca gcgagtattt gttgttactt
     9241 gaccagcccg caaacagcac cgcgtaaacc gccagacctg ccatcatcag gaagaacaaa
     9301 atcccgatgt tcaggtcggc aaccacccaa cccggactga ctggcacaat tgcaaaggcc
     9361 agcagcagcg aggtaaaggc aatcatcggt gccagggtaa agatgacgcg atccgagaat
     9421 ttcgggatcc agtcttcttt aaagaacatt ttgatcatgt ccgcaaccag ctggagcgaa
     9481 ccgccccagc caacacggtt aggtccataa cggttctgga acagacccag caggcgacgt
     9541 tcgccaaagc tcatgaatgc cccacaggtg acaaccacca gcaggatccc caccgctttg
     9601 aggatggtca gcaggatctc aatcagttcc ggtgatatcc aactcattgt tgtgcctcct
     9661 tgagatcctc aagatgcgcg ccagccagca ccggagcaat gccggacata cccatcggca
     9721 agcccacctg ccctgccgtc agtccttcgg cgatttcaac cggcagcgtg accgtgttgc
     9781 catcgtaact aaaggagacg cgtgtacctg cgttcacacc caacttcgcg gcatccgctg
     9841 ggttgagttt gatgtacggc tgcggcatac ggctctggaa gaccggagca cgctgtgaca
     9901 attcatcgct gccaaacagg tggtagtacg gcgcgatacg ccatttcccg tcctgcggct
     9961 ggaagcgtgc cggtacgctg gtgaagtaat ccagaccgtt ttcgctggtt tcaaacagac
    10021 gcacgcccgg atcgccaaag cgcagtttgc cgcccacttc gtcctggaat ttgttccacg
    10081 cctgcgggga gttccagccc ggcgcccagg caaacggcac ttgcgaacgg tgcgcggtcg
    10141 gctggttgtt accttccatc gagaaggtga acatggtgtc aatatcctgc ggctgacgcg
    10201 gctcatgaac gctgatattg gcgcgcatgg cggtacgacc gctgtaacgg tgcggttcac
    10261 gggccagttt ctgcccacga atacggaatg tcgcatccgg cgcagcatct ttgatacccg
    10321 ccagttccgg gattttcgcc acaacagcgt caattacatg gtcgagctgc gtccagtcca
    10381 cttcacggct cagcaaggtg ctgtgcagcg agtgcagcca gcgccagctt tccagcatga
    10441 cagttttgct gtcgtaataa gccggatcgt aaacctggaa gaaacgttgg gcgcgacctt
    10501 cattattaat taccgtaccg tcgctttcag caaagctggc agcggaaagc accagatggg
    10561 cgttttccat gatcgctgtg cgttgatgat caaccaccat caccagcggc gctttagcca
    10621 gcgcagcatt cacgcgggta gcagaagcgt aacgatgcag atcgttttcc aacaccacca
    10681 ccgcgtcggc gcgtccggtt tccagttcgg ttaacgcttc ttcaagcgaa ccgccaccca
    10741 taatgcccag ccccatgctg ttgacggaac gggcaatcat ggtgatacca acgtcagcgc
    10801 cgcgaccttt cagggctttc gcgacgttag ccgcagcctg aatcacctct atgctaccgg
    10861 cgttcgtccc ggagataatc aacggtttct tcgcacctgc cagtgcctgc acgatgacgt
    10921 cgattttgtt ttgcagctca ggttcgatac cgtcaaccgc tggtgcggag ttatccagcg
    10981 catgggcgat ggcaaaacct aaacgcgcct gatcttcaac cggtgcgcgg taagtccacg
    11041 ccgcgatatc atccagacga gtgtcatcaa cgttggtaac aaacagcgga tgcttcgcac
    11101 gttgaccgat gttgaggatt gccgcaatct gccagtcagc cactttctgt gctgcagcca
    11161 tttcgcgcgc tttacctttc accgcctgac gcactgccag tgcgacgcgc gcgccggtct
    11221 gggtaacgtc ttcgcccagc accagtaccg catcgtaaga ttcgatttcg cgcagagccg
    11281 gagtataaat gccgccttca cgcagcactt tcagcgccag ttgcagacgt tcctgctcac
    11341 cgtgagcgat accggtgtag aagttttctt cgcccaccag ttcacgcagc gcaaagttgc
    11401 tttccacgct ggcacgcgga gaaccaatac cgatcacttt cttcgactga cgcagaatat
    11461 ctgccgcgcc ctgcattgcc tgttcggcgt tgagggtaat gaaatcatcg ccacgacgct
    11521 gtactggctg acgcggacga tccttcaggt tgacgtaacc gtaaccgaaa cgaccacggt
    11581 cgcagaggaa gtagtggttt accgtaccgt tgtaacggtt ttcgatacga cgcagttcgc
    11641 cgtaacgttc accggggctg atgttacagc cgatggaaca ttgctggcag atgctcggcg
    11701 caaactgcat atcccattta cggttgtaac gctcggagtg cgttttgtcg gtaaatacgc
    11761 cggtcgggca aatttcgacc aggttaccgg aaaattcgct ttccagcgtg ccgtcttccg
    11821 ggcgaccgaa gtagacgttg tcgtgcgcgc cataaacgcc cagatctgta ccgtcagcgt
    11881 aatctttgta gtaacgcaca cagcggtagc aggcgatgca gcggttcatt tcgtgagaga
    11941 tgaatggccc caaatcctga ttacggtggg tacgtttggt gaaacggtag cgacggaagc
    12001 tgtgtccggt catcacggtc atatcctgaa gatggcagtt accgccttct tcacataccg
    12061 gacagtcgtg cgggtggttg gtcattaacc actcgaccac actttcacgg aactgtttcg
    12121 cttcttcgtc gtcaatggaa ataaaggtgc catcggaagc cggtgtcata caggacatca
    12181 ccaggcgacc acgcgtgtct tccgcgtttt ggtattgctt caccgcacac tggcggcaag
    12241 caccgacgct tcccagcgcc ggatgccagc aaaagtaagg aatatcaagg cccagagaca
    12301 gacaagcttc cagcaggttg tccgctccgt tgacctcgta ttctttgccg tctacatgaa
    12361 ttgtagccat tagcatgctt ccagttttct cggtcaggga ccgagcgtta atcgaaattc
    12421 ggttaccagc gctctttcag caggttcggc tgaatcccat taatcaaatg ggtattgctg
    12481 aacggctgtt tgattcccgc ctcaaattct tcgcggaaat atttgatggc gctctgtaac
    12541 ggctccactg caccaggtgc gtgggcacag aaagttttac ccgggcctaa gaatcgacac
    12601 agttgctcaa gtgtttcgat atcgcccggc tggccttcgc cacgctccag cgcacgcaga
    12661 attttcacgc tccacggcag accgtcgcgg cacggcgtac accagccgca ggactcacgg
    12721 gcgaaaaact cttccaggtt acgcaccagc gacaccatgt tgatctcatg gtcaaccgcc
    12781 atcgccagcg ccgtacccag acggctgccc gctttaccga tactttcgaa ttccatcggc
    12841 agatcaaggt gcgcttcggt caggaagtca gtccccgcgc cgcctggctg ccaggcttta
    12901 aatttcagac catcacgcat accaccggcg taatcttcga ggatctcgcg tgcggtggtg
    12961 ccgaacggca gttcccacag tcccggattt ttcacccgac cggagaagcc catcagcttg
    13021 gtgccagcat ctttactttt cgagatgttc tgataccact ccacgccgtt agcgaggatc
    13081 gccggaacgt tacacagggt ttcgacgttg ttgacacagg tcggtttgcc ccatacgccg
    13141 gaggttgccg ggaagggggg tttcgagcgt gggttagcac gacgcccttc cagggagttg
    13201 attaacgctg tttcttcccc gcagatgtag cgccctgccc cggtatggac gaacagttcg
    13261 aagtcgaaac ctgttcccat aatgtttttg ccaagcaagc ccgcttcagt ggcttcggca
    13321 atagcgcggc gcagatttac tgccgcttcg atatattcgc cacgcaggaa gatgtagcca
    13381 cggtaagctt tcagcgcaaa cgcggagatg agcatacctt ccaccagcag gtgcggcagt
    13441 tgctccatca acaggcggtc tttataggtg cccggctcca tttcatcggc gttacacagc
    13501 aggtaacgga tgttcatgga ttcgtctttc ggcatcaggc tccacttcag gccagtcgaa
    13561 aagcccgcgc cgccgcgccc tttcagacca gcgtctttta cctgattaac gatttcgtcc
    13621 ggagacagcc cggtcagcgc cttacgcgcc ccttcgtaac cgtttttgct gcggtattcg
    13681 tccagccaca ctggctgttt gtcatcgcgc agacgccagg tcagcggatg cgtttcggga
    13741 gtacgggtaa tgtttttcat ttataccgct ccagcagttc agggatcgct tccggggtca
    13801 gatgcgcgtg agtgtcctca tcgatcatca tgtttggccc tttatcacag ttccccaggc
    13861 agcaagttgg cagcagcgta aagcggccat caaatgtcgt ttgccctggt ttgatgttca
    13921 gctttttctc gagcgccgcc tgaataccct gatacccgtt gatatgacag accacgctgt
    13981 cgcaataacg gatcacatgg cgaccaaccg gctggcggaa gatctgactg tagaacgttg
    14041 ccacaccttc gacgtcgctt gccggaatac ccagcacatc ggcgatcgcg tggatcgcac
    14101 catccggcac ccagccacgc tgcttctgaa cgattttcag cgcttcaatg gacgccgcac
    14161 gcgggtcttc gtagtggtgc atctcgtgct caatcgcttc acgctctgcc gcactcagct
    14221 caaaagcctc ggtttgtggt tgttgattct cgtgcataat tagcggtcca catctgacat
    14281 aacaaaatcg atactgccca gataaacaat caggtcagac accaagctgc cgcggatcgc
    14341 cgccggaatt tgctgcaaat gcgcataact cggcgtgcgg atacgagtgc gataactcat
    14401 ggtgctaccg tcgctggtca ggtagtaact gttgatcccc ttggtcgcct caatcatctg
    14461 gaaagattca ttggcaggca tcaccggccc ccacgacact tgcaggaagt gggtgatcag
    14521 ggtttcgata tgttgcagcg tgcgctcttt cggcggcggc gtggtcagcg ggtgatccgc
    14581 tttgaacggg ccttccggca tgttgttgag gcactgctca agaatgcgca gactctggcg
    14641 cagctcttcc actttcagca ttacgcgggt gtagcagtca gaaacaccac caccaaccgg
    14701 gatttcaaag tcgaagtttt catagccaga ataaggacgc gccttacgca cgtcgaagtc
    14761 gatcccggta gcacgcaggc ccgcgccagt ggtgccccac tccagcgcct ctttcgcgcc
    14821 ataggcggca acgccctggg tacgaccttt cagaatggta ttttgcagcg ccgctttctc
    14881 gtaagacgcc agacgtttcg gcatccagtc gaggaactca cgcagcagac gatcccagcc
    14941 gcgcggcagg tcgtgcgcta cgccgccaat acggaaccac gccgggtgca tacggaaacc
    15001 cgtgatcgct tccaccagat cgtaaatttt ctgacgatcg gtaaaggcga agaacacggg
    15061 cgtcattgcg ccgacgtcct gaataaaggt cgagatgtac agcaggtggc tgttgatacg
    15121 gaacagttcg gagagcataa cgcgaatgac gttaacgcga tccggcacgg tgatcccggc
    15181 cagtttctct accgccagca cgtaaggcat ttcgttaacg cagccgccga ggtattcaat
    15241 acggtcggta tacggaatgt agctgtgcca ggactggcgc tcgcccattt tctccgcacc
    15301 acggtggtgg tagccgatgt ctggtacgca gtcgacaatc tcttcgccgt cgagttgtaa
    15361 aacgatacgg aaagccccgt gcgccgacgg gtggttcgga ccgaggttga ggaacatgaa
    15421 gtcctcgttt tcggtgccgc gcttcatccc ccactcttcc ggtttgaagg tcagggcttc
    15481 catctccaga tcctgtttgg ctttggtcag ctcaaacggc gagaattcgg tagcgcgcgc
    15541 cgggtaatct ttacgcagcg ggtgaccttt ccaggtttgc ggcatcatga tgcggcgcag
    15601 gttcgggtga ccgtcgaaag taatgccaaa cagatcccag gtttcacgct cataccagtt
    15661 agcgttcggg aacagtttgg tgaaggtcgg tacgtgcagg tcgttttctg ccagcgccac
    15721 cttcagcatg atgtcgcggt tacgatcgat agaaatcaga tggtagaaaa cggaaaaatc
    15781 cgcggcaggt aacccttcgc ggtgtgtgcg cagacgttcg tccatgccgt gtaagtcaaa
    15841 cagcatgacg taaggtttcg gcagtttctt taagaaatcg ccaacttcca gtaattgttc
    15901 acgcttgatc cacacaacgg gaaccccggt gcgagtcgcc tgaacagtaa aggcatccgg
    15961 cccaaaacgg ttgcgcagtt cgccaattac cggatcatca agatgatcgc gggtctgcca
    16021 ggcgggttct tgcgcggtta agtcggtcat attgttcacc attgcaaatg gtccgtggtg
    16081 actgtcggcg cagtaagctt cgcgaaatag atttaggaat aagcgaagaa aaatcccttt
    16141 gccgacaggc gcaaattaaa tctcgtcagg tgtacgcagg ttggttacgg caatgcgttc
    16201 accgcgcttg cgttcgcgct ctgattgcat attggcgcga taaacgccct gatcgccaac
    16261 cacccaggag agcggacgac gttctttgcc gatagattcc tgcaacagca tcagtgcctg
    16321 catgtacgct tcaggacgcg gcgggcagcc cgggatatac acatcaaccg ggatgaattt
    16381 atcgacgccc tgcacaacgg aataaatatc gtacatacca ccagagttgg cacaggcccc
    16441 cattgagata acccattttg gttccagcat ctggtcatac agacgctgaa taaccggtgc
    16501 cattttggta aagcaggttc ctgcaaccac catcaggtca gcctgacgcg gcgaagcacg
    16561 caatacttct gcgccaaaac gcgccacgtc atgcaccgcg gtaaacgaag tcaccatctc
    16621 aacgtaacag caggaaagac cgaagttata cggccaaatt gagtttttac gaccccagtt
    16681 aaccatgtca ttgagcttgc ccataaacac gtttttgtta acttcttgct ccagagggtc
    16741 ggttacgatc tcctgctttt gcagggggta acggtcgttc tcaccgttgg gatctatgcg
    16801 ggtgagcgta taatccatct taatgcctcg cggttagcgt tgacgattag cgatactgtt
    16861 cgtttccggg ttcatacact cgcggcgtga acgcgcgggc gtccagtcca gcgcgccaat
    16921 acgcaccaga taaaccagac ctgccagtaa cacaaaaata aaaattgcag cttccacaaa
    16981 gcctacccag ccgctttcgc ggatagaggt tgaccatgcg aacagataca gcgcttcaac
    17041 gtcgaagata acgaagaaca tggccaccag ataaaacttg gcggacaggc gtaagcgggc
    17101 ggagccgacc gaatcgatac cggattcaaa cggcacgttt ttcgacctcg cgcgtgcgcg
    17161 accgcctaaa aaccaaccgc ctaccagcat caggcaacac aggccaatgg caacgataag
    17221 aaagatagcg aatgcccagt gatgagcgat gacttcagtg gatgttgaca tactcattgc
    17281 ttactcatca aaagtagcgc cagattcact gctcttcacg gcagatggac gccacatcga
    17341 ttcatgggga ggaataaaaa aaaccttaca atcactgtag aaattctttt atacagctaa
    17401 ttgatgtggt cttttactcc tttctataac cttttgtcaa ctttaacaaa agtttcttca
    17461 cattagttta cataatatca acaccattag catttaatgc cctttcaccc cagatccttg
    17521 acgactccag gataattaga tgttgttgaa tcgtgtccgt tgtgaagcaa tggaaaaaat
    17581 acgggtctat tttgacagga atttgtgtcg attcctcccc ccaaaagaga gtattttctt
    17641 gatctgtgac acgcttttgt tattccataa caaaaacgca gcaacaaatt tacgtatttt
    17701 ttaacatcat tgtagcaggt gatttttttc aggcgattat ttgtgcgttc gggacgtgaa
    17761 tctctggtga ttgaaaaata aacagttttg tacgttctgc actatgcgag gaaggatttt
    17821 tactaaaaaa aagccgctgg ggtttaaaac acccccagcg gctcgttttt tacactattg
    17881 tctcaggaat tatctatcgt ccgtcgatta ctcgatatcc ctttcaatca acaatgaatc
    17941 atccccttcc ggagcagaca ttgggctgta ttgccacgga ttatggtagc tctccatcgc
    18001 ctgatagatc acctgggcca gctcattatt actggacggg tcatagcaca gcaaatattc
    18061 ggtatcaggt agcggcggta agccatctac tccactcagc acacgcaggt ccgggctcat
    18121 catctcaacc ggccttgccg tcacgccaag accggctttc actgccgcac gaacggccgg
    18181 aagcgtcgag gcgacataag ccagtcgcca tggaatatct gctttattaa gcgtcgccag
    18241 caccatatcg cgaaacggac ttggatcatc cagcaataca agagggatcg gctcgccttt
    18301 ttgcaatacg tattccgctg cgcagtacca gtgtgttggc gaggtacgca ggttcaatgc
    18361 cttaaacgct gaaggacgat gggtggttac catcaaatcc acttcctggg attccagcat
    18421 ttctgccata taggcattac gtttaacgcg gacatccagc gctaatttcg gataaaccga
    18481 actcacgcga tttaacagga aaggtaagat cgtatcggca gattcatctg aagcaccgat
    18541 agttaacacg ccctgaagat tactgaacat taatgatgag caggcctcat cattaaaacg
    18601 caggattttc ctggcgtaac caagaagttg aatgccatgt tcagttaaca gtttgttgcg
    18661 accgtgacga gcgaacagtt ctttcccaac gagttgttcc agacgctgca tttgctgact
    18721 tacggcggac tgagtacgac acacagcggc agctgcggca gcaaaagtgt tcagatcggc
    18781 aacagcaaca aatgttctca gcagatcgag gtcgaggtta attatcggac gatttgcact
    18841 tatcatatat tatcacttac tggcggctca tactgagctg gttaatgctg tgcacacaca
    18901 aacaagcaat tccatttgta atgtgcctcc ctggcagttt catccggaac ccggacgaaa
    18961 gtaaaaatgc atatgagttg cactaaaaaa gcgactcaca ttgttccgtt ataatgcctg
    19021 aagtagatca cagaatatat cttcagggat cgcatatcta ttaagttact cactcttttc
    19081 tatttatgac atgcgcgtgt ttgtataaat gtaaatgtga gtccttgttc cactctcgtg
    19141 cagcatcgct ggtcatacgc gaacacgtac aaacagcaat ggtgaagcgg catcaagagc
    19201 agggatccgt catttatgcg agcattttac cccaaaaacc ttttatttat aagggtcatt
    19261 gcgaattatc tgatgcaaag ttatgttatg ttatgttagg caaagtaatc ttctattatt
    19321 aataagcaca tcaaaacctt tttgaatatt aaataataat taattagcat catcctcatt
    19381 cattaattcc gcttaacaat agtttcacac cttaccccca ttgcgacacc tcggaaatca
    19441 tcacgctgtt aaactctccg taatgcggaa atatcatcat cgcgtttcat tagtgaattc
    19501 ttaaatgagg cattttcaca caattatctt acagataaaa aaaccatact tgcaattaag
    19561 aattagaaca attcactata taacattaca tgtaaagcat atacacctaa ttattttgtc
    19621 attattaagt tattaacagc acaatcgagc cttcccctct ggcaaaatct tattctgcag
    19681 accttcaaaa caccgtcctg ggggagtaca ttgttctaag ctgacttcca cggcagggag
    19741 tggcgataac agcaaaaaag gtcaagattc atgtccccca ttgaaaaatc cagcaaatta
    19801 gagaatgtct gttatgacat ccgtggtccg gtgctgaaag aagcaaaacg cctggaagaa
    19861 gaaggtaaca aggtactgaa actgaacatc ggcaacccag ccccattcgg ttttgacgcg
    19921 ccagatgaaa tcctcgttga cgtgatacgc aacctgccta ccgctcaagg gtattgcgat
    19981 tccaaagggc tttactccgc gcgtaaagcc atcatgcagc actaccaggc tcgtggcatg
    20041 cgtgatgtta ccgtggaaga tatttacatc ggcaatggtg tatcggagct tatcgttcag
    20101 gcaatgcagg cattgctgaa cagcggggac gaaatgttgg ttcctgcacc agattaccca
    20161 ctctggaccg cggcggtttc gctttccagc ggtaaagcgg tgcattatct ttgcgatgaa
    20221 tcctctgact ggttcccgga cctcgatgat attcgcgcta aaattacgcc tcgtacgcgt
    20281 gggattgtta ttatcaaccc aaataaccca accggcgcgg tatattccaa agagctttta
    20341 atggagattg tggagattgc acgtcagcat aatctcatta tcttcgccga tgaaatttat
    20401 gacaaaattc tctacgacga tgctgagcat cactcaattg cgccgctggc acctgacctg
    20461 ctgaccatta cctttaacgg actgtcgaaa acgtaccgcg ttgcaggctt ccgtcagggg
    20521 tggatggtgt tgaacgggcc gaaaaaacac gccaaaggct acatcgaagg tctggaaatg
    20581 ctggcttcaa tgcgcctgtg tgctaacgtt cctgcgcaac acgccattca gaccgcgctg
    20641 ggtggttatc agagcatcag tgaatttatt acccctggcg gtcgtcttta tgagcagcgt
    20701 aaccgcgcgt gggaactgat caacgatatt ccgggcgttt cctgcgtgaa acctcgtggt
    20761 gcgctgtata tgttcccgaa aatcgacgcc aaacgcttta acattcatga cgatcagaaa
    20821 atggtgctgg atttcctgtt gcaggaaaaa gttctgttgg tgcaagggac ggcattcaac
    20881 tggccgtggc cggatcactt ccgcattgtc acgctaccgc gtgtcgatga tatcgagctg
    20941 tctttgagca agttcgcgcg tttcctttct ggttatcatc agctgtaatc ttaatttcac
    21001 tgccggagat tgcatccggc agtgttatcc cgccacaatg acctgatgat gtcatcatac
    21061 gtaaggtcac tatgaaacag agccattttt ttgcccatct ctcccgcctg aaactcatta
    21121 accgctggcc gctaatgcgc aacgtgcgga cggaaaatgt gtccgaacac agtttgcagg
    21181 tagcgatggt cgcccatgcg ctggcagcta tcaaaaatcg gaaatttggc ggtaatgtca
    21241 acgccgaacg tatcgcttta ctggcgatgt accacgatgc ctcagaagtg ctcaccggcg
    21301 atctccctac tccggtgaaa tacttcaatg cgcaaatcgc tcaggagtac aaggctattg
    21361 aaaaaatcgc tcagcagaaa ctggtcgata tggttccgga agaactgcgg gatatctttg
    21421 cgccgttaat tgacgagcat gcatatagcg atgaagaaaa atcgctggtg aaacaggcag
    21481 atgcactgtg tgcatatctg aaatgtctgg aagaactcgc ggccggaaat aatgaattct
    21541 tgctggcaaa aacgcgactg gaagcgacgc ttgaagcgcg tcgcagccag gagatggact
    21601 acttcatgga agtatttgtt cccagcttcc atctttcgct cgatgagatt agccaggatt
    21661 caccgctgta agcagccgga gtctgcgtcg catcaggcaa taagcgccgg atgcgacatc
    21721 aggctcttgt caaaacggaa acagcatcgg gatcatcacc acacaaaccg ccatcacgat
    21781 aatggtgaac ggtaccccca acttcacaaa gtcactgaag ctgtaatttc ccggacctaa
    21841 aaccagggtg ttaacaggtg aagacaccgg tgtcataaag gcggcggatg ctgccatcgc
    21901 cacgaccatc gcaaatggat aaggcgacac tcccatcgtt ttggcagcag ccagcgcaat
    21961 cggagccatc aacaccgccg tcgcggtatt agagataaac agcccaataa ccgccgacaa
    22021 gacaaacaaa caccccagca tcatatgtgg cccataaccg ccgccaatat ccatcagccc
    22081 tttcaccgcc agcgcgacac ctcccgtttt ctgtaatgcc acagcaaacg gcatcatccc
    22141 aacgatcaaa ataatgctcg gccagtgaat ggatttatag gcgctttcag catctataca
    22201 gcggaatttc cccatcagca ggcaggcgat gatagcggca acaggattag gaatttcatt
    22261 tgtcagcatt aacgccacca ttagcaccag acagaaaatg gcatggggtg cctggctgtg
    22321 cgcgggcgat gcttcactca cctcttccgg taagttcagc gctacgaagt cgcggccctg
    22381 tttggccagc ataccgatca gtttccagtt acccacaacc aggatgatat cgcccagcag
    22441 cagaggctca tccgccagcg aaccttccag cgccacgcca ttgcgcttga gccccaccac
    22501 attcagtccg tagcgggtac gaaaaccaat ttcgcgcacc gatttaccaa tcagttctga
    22561 ctcaggaatt aatgaaatct ctgccatgcc cacatcaagg gcctggtcag aaaaatactc
    22621 gccgcgcagt accatcggct ccagcaattg ctcactacaa aattgccgga gatcgacatc
    22681 agccgcagac atatcaataa gcaaaacgtc acgcgcgcga aattcagaaa ccccattaac
    22741 gttcacgata acgcgacgaa aacgccgcca gcgttcaaca ccgatgacgt tagcgccata
    22801 acgctcacgt aatttgagat catccagccg ttgacctatc attggcgatc cggggcgaat
    22861 agccagacgt cgcgcacgcc cggtcagtcg atattcacgg ataagatcgc gaaaggttcg
    22921 acgcgtccag ccttcgcgct gcggggtctg ggtatcccct ttcagcatga aacgcatcac
    22981 taacatatac aaaataccca gcaccagcac gaccaggcca ataggtgtta cgctaaagaa
    23041 actgaagccg tgatagcctt cacgcagcaa ttcactgttg actaccaggt tcggcggcgt
    23101 cgccaccagc gtcatcatgc cgctaatcag cccggcaaaa cttaacggca tcatcagacg
    23161 tgacggcgaa gtttgcatac gcatggcaac gcttaacacc acgggaataa agatagcgac
    23221 aacgctggtt gaactcataa acgcgccaag ccccgcgacg gtcagcatca acaaaaccaa
    23281 cattttgatt tcactgttgc ccgccacttt gaccagccat gttcccatta cggtggcaac
    23341 accggtacgg accaaaccat cgccaataat aaacaaggcg gcaatcagga caacgttagg
    23401 atcagaaaag ccggaaaata cttcggggac cgtcagcgtt ccgcttaatg caaacgcgac
    23461 aataacaaac aaagcgaccg catccatacg cactctgccc gtcgcaaaca agacgatggc
    23521 aacggccagt aatgaaagaa cccaaatcaa ttcaccgttc acaacttatc cttgttaatt
    23581 gagggggatg acttgattct gccataaaaa agccccgacg agacggggct aaatcatgat
    23641 caggtgtttc actgaataat aacatcgcca tttggctgtt tggtcacagt aatttgctcc
    23701 agactgtgga ggaccaaatc gacctcattc aggcgcgggg tatctgccgg agcgttaacc
    23761 gcaatgacat ggcaacccgc cgctaggcca gaaagcacgc cagcgggagc atcttccacc
    23821 accacacact cctgcggcgc aagccccagc agctgcgcac ctaacagata cgcatcaggt
    23881 tctggttttc cgcgcttcac tcgctcagcg gttacaaaca cctctggtgc gggaagcccc
    23941 gctattttat ggcgcgctcg cgctaccggc atggagccag aagtcacaat ggcccacgga
    24001 atacctgctt tattcaaatg attgagtaag gcgattgccc caggaagcgc ggtaatacct
    24061 tcggtttccg tggcctcgat ttgctccaaa cgcgtaaact cggcggcaat atcagcctcg
    24121 gatttgcccg ccataaaatg gcgcaaagag gtgatcgcct gtttaccgtg aatgaaagcc
    24181 agcacctctt ccggcgctaa cccatgacgt ctggcccagt tgctccacgc ccgttctacc
    24241 gcaggcaggg aatccaccag cgttccatca agatcaaaca gaaaaccttt gcaccgcacg
    24301 cgggcctcct caggcgttga taatttgatt aatttcgttg gcgctcaaat ggtactgacg
    24361 cgggcaggca tgccacacat taagcatgcg ctgatatttt tcccacattg gcgtctgggc
    24421 gttaaaacca tgagtcccag cgtcaaaatg ggtatagcgc ccttccacat taaccataaa
    24481 gcggacataa ccgaggtaac gtgcttcagt ggcggcgtca aagccgagga aggtgacacg
    24541 acgttcatcg atggattgct ggtcctgcaa attagaccag gaaacatgca acgcatgata
    24601 catctccata atgtcgatga tagtgcggca ggtttcttct ttcagctcgc caaactcgcg
    24661 atccaattca cgcatctgta atccgtaacc acgctcaata attgtttgca ggcgacggta
    24721 acgttcagca tttgccggat cgagcatagt catcatcttg tactgattag acaaaataag
    24781 acgttgcgcg ttggtcattt ccattgttga ctcctgtatc actctactac ggtgaaaaaa
    24841 aagaaggctg agtatgcctt cttttatatg cgtaatcagg ggtcaattac aaatcatcaa
    24901 ggaaagtttt atccagttgt ttgaaggcgc gcttaagcgt gtcagctaat gcctggtaat
    24961 caggcttgcc ttcaacgggt gccaacacct gtccagactc ctgcaattta ccgcgaactt
    25021 cataaaacca gttaaggatt gcagggggta atggcgttac agaacgcttg cccagccacc
    25081 acaatccctg catgggtaaa cttaaggcga acagtgcagt ggcaactgcc ggcccaagca
    25141 gaccgcccag ggcaatctgc cagcagagag taaatacggc gatcggcggc ataaaacgga
    25201 tcgcataacg cgtcatcttg ataacgcgat tttcgacaaa gaccggggca aggcgttttt
    25261 ccagtggcca cgtctttgag taatgctgtc cgcggcgaaa caagctaaaa aaattaacag
    25321 aacgattatc cggcgttgac atgcttcacc tcaacttcac atataaagat tcaaaaattt
    25381 gtgcaaattc acaactcagc gggacaacgt tcaaaacatt ttgtcttcca tacccactat
    25441 caggtatcct ttagcagcct gaaggcctaa atagtacata ttcattgagt cgtcaaattc
    25501 atatacatta tgccattggc tgaaaattac gcaaaatggc atagactcaa gatatttctt
    25561 ccatcatgca aaaaaaattt gcagtgcatg atgttaatca taaatgtcgg tgtcatcatg
    25621 cgctacgctc tatggctccc tgacgttttt ttagccacgt atcaattata ggtacttcca
    25681 tgtcgagtaa gttagtactg gttctgaact gcggtagttc ttcactgaaa tttgccatca
    25741 tcgatgcagt aaatggtgaa gagtaccttt ctggtttagc cgaatgtttc cacctgcccg
    25801 aagcacgtat caaatggaaa atggacggca ataaacagga agcggcttta ggtgcaggcg
    25861 ccgctcacag cgaagcgctc aactttatcg ttaatactat tctggcacaa aaaccagaac
    25921 tgtctgcgca gctgactgct atcggtcacc gtatcgtaca cggcggcgaa aagtatacca
    25981 gctccgtagt gatcgatgag tctgttattc agggtatcaa agatgcagct tcttttgcac
    26041 cgctgcacaa cccggctcac ctgatcggta tcgaagaagc tctgaaatct ttcccacagc
    26101 tgaaagacaa aaacgttgct gtatttgaca ccgcgttcca ccagactatg ccggaagagt
    26161 cttacctcta cgccctgccg tacaacctgt acaaagagca cggcatccgt cgttacggcg
    26221 cgcacggcac cagccacttc tatgtaaccc aggaagcggc aaaaatgctg aacaaaccgg
    26281 tagaagaact gaacatcatc acctgccacc tgggcaacgg tggttccgtt tctgctatcc
    26341 gcaacggtaa atgcgttgac acctctatgg gtctgacccc gctggaaggt ctggtcatgg
    26401 gtacccgttc tggtgatatc gatccggcga tcatcttcca cctgcacgac accctgggca
    26461 tgagcgttga cgcaatcaac aaactgctga ccaaagagtc tggcctgctg ggtctgaccg
    26521 aagtgaccag cgactgccgc tatgttgaag acaactacgc gacgaaagaa gacgcgaagc
    26581 gcgcaatgga cgtttactgc caccgcctgg cgaaatacat cggtgcctac actgcgctga
    26641 tggatggtcg tctggacgct gttgtattca ctggtggtat cggtgaaaat gccgcgatgg
    26701 ttcgtgaact gtctctgggc aaactgggcg tgctgggctt tgaagttgat catgaacgca
    26761 acctggctgc acgtttcggc aaatctggtt tcatcaacaa agaaggtacc cgtcctgcgg
    26821 tggttatccc aaccaacgaa gaactggtta tcgcgcaaga cgcgagccgc ctgactgcct
    26881 gatttcacac cgccagctca gctggcggtg ctgttttgta acccgccaaa tcggcggtaa
    26941 cgaaagagga taaaccgtgt cccgtattat tatgctgatc cctaccggaa ccagcgtcgg
    27001 tctgaccagc gtcagccttg gcgtgatccg tgcaatggaa cgcaaaggcg ttcgtctgag
    27061 cgttttcaaa cctatcgctc agccgcgtac cggtggcgat gcgcccgatc agactacgac
    27121 tatcgtgcgt gcgaactctt ccaccacgac ggccgctgaa ccgctgaaaa tgagctacgt
    27181 tgaaggtctg ctttccagca atcagaaaga tgtgctgatg gaagagatcg tcgcaaacta
    27241 ccacgctaac accaaagacg ctgaagtcgt tctggttgaa ggtctggtcc cgacacgtaa
    27301 gcaccagttt gcccagtctc tgaactacga aatcgctaaa acgctgaatg cggaaatcgt
    27361 cttcgttatg tctcagggca ctgacacccc ggaacagctg aaagagcgta tcgaactgac
    27421 ccgcaacagc ttcggtggtg ccaaaaacac caacatcacc ggcgttatcg ttaacaaact
    27481 gaacgcaccg gttgatgaac agggtcgtac tcgcccggat ctgtccgaga ttttcgacga
    27541 ctcttccaaa gctaaagtaa acaatgttga tccggcgaag ctgcaagaat ccagcccgct
    27601 gccggttctc ggcgctgtgc cgtggagctt tgacctgatc gcgactcgtg cgatcgatat
    27661 ggctcgccac ctgaatgcga ccatcatcaa cgaaggcgac atcaatactc gccgcgttaa
    27721 atccgtcact ttctgcgcac gcagcattcc gcacatgctg gagcacttcc gtgccggttc
    27781 tctgctggtg acttccgcag accgtcctga cgtgctggtg gccgcttgcc tggcagccat
    27841 gaacggcgta gaaatcggtg ccctgctgct gactggcggc tacgaaatgg acgcgcgcat
    27901 ttctaaactg tgcgaacgtg ctttcgctac cggcctgccg gtatttatgg tgaacaccaa
    27961 cacctggcag acctctctga gcctgcagag cttcaacctg gaagttccgg ttgacgatca
    28021 cgaacgtatc gagaaagttc aggaatacgt tgctaactac atcaacgctg actggatcga
    28081 atctctgact gccacttctg agcgcagccg tcgtctgtct ccgccagcgt tccgttatca
    28141 gctgactgaa ctggcgcgca aagcgggcaa acgtatcgtt ctgccggaag gtgacgaacc
    28201 gcgtaccgtt aaagcagccg ctatctgtgc tgaacgtggt atcgcaactt gcgtactgct
    28261 gggtaatccg gcagagatca accgtgttgc agcgtctcag ggtgtagaac taggtgcagg
    28321 cattgaaatc gttgatccag aagtggttcg cgaaagctat gttggtcgtc tggtcgagct
    28381 gcgtaagaac aaaggcatga ccgaaaccgt tgcccgcgaa cagctggaag acaacgtggt
    28441 gctcggtacg ctgatgctgg aacaggatga agttgatggt ctggtttccg gtgctgttca
    28501 cactaccgca aacaccatcc gtccgccgct gcagctgatc aaaactgcac cgggcagctc
    28561 cctggtatct tccgtgttct tcatgctgct gccggaacag gtttacgttt acggtgactg
    28621 tgcgatcaac ccggatccga ccgctgaaca gctggcagaa atcgcgattc agtccgctga
    28681 ttccgctgcg gccttcggta tcgaaccgcg cgttgctatg ctctcctact ccaccggtac
    28741 ttctggtgca ggtagcgacg tagaaaaagt tcgcgaagca actcgtctgg cgcaggaaaa
    28801 acgtcctgac ctgatgatcg acggtccgct gcagtacgac gctgcggtaa tggctgacgt
    28861 tgcgaaatcc aaagcgccga actctccggt tgcaggtcgc gctaccgtgt tcatcttccc
    28921 ggatctgaac accggtaaca ccacctacaa agcggtacag cgttctgccg acctgatctc
    28981 catcgggccg atgctgcagg gtatgcgcaa accggttaac gacctgtccc gtggcgcact
    29041 ggttgacgat atcgtctaca ccatcgcgct gactgcgatt cagtctgcac agcagcagta
    29101 atctcgtcat catccacagc tttgcgctgc ggatatctga accggaaata atcactattt
    29161 ccggtttttt attctcttaa tttgcattaa tcctttctga ttatcttgct taactgcgct
    29221 gcatcaatga attgcgccat ctcactttgc atacttacca ctttgttttg tgcaagggaa
    29281 tatttgcgct atgtccgcaa tcactgaatc caaaccaaca agaagatggg caatgcccga
    29341 tacgttggtg attatctttt ttgttgctat tttaaccagt cttgccacct gggtagttcc
    29401 ggtggggatg tttgacagtc aggaagtgca gtatcaggtt gatggtcaaa caaaaacacg
    29461 caaagtcgta gatccacact catttcgcat tctgactaac gaagcaggcg aacctgagta
    29521 tcaccgcgta cagctgttca cgacgggcga tgaacgcccg ggcctgatga acttcccgtt
    29581 tgaagggtta acctcaggat cgaaatacgg gacagccgtt ggcatcatca tgtttatgct
    29641 ggtgattggc ggcgcgtttg gcattgtgat gcgtacagga accattgata acggtatcct
    29701 ggcgcttatt cgccataccc gcgggaatga aattctcttt attcctgcgc tgtttattct
    29761 gttttcactt ggcggcgcgg tatttggtat gggagaagag gccgtcgcct ttgccattat
    29821 catcgcaccg ctaatggtcc ggctgggcta tgacagtatt accaccgtcc tggtgactta
    29881 tattgccacg caaatcggtt ttgccagttc gtggatgaac ccgttttgtg tggttgttgc
    29941 tcaggggatt gccggcgttc cggtgctttc tggctccggg ttgcgcatcg tggtgtgggt
    30001 tatcgccact ctgattggcc tgatctttac catggtgtac gcctcacgag tgaaaaagaa
    30061 tcctcttctg tcacgcgtgc atgagtccga ccgcttcttt cgtgaaaagc aggcggatgt
    30121 tgaacaacgt ccgtttacct ttggtgactg gctggtattg attgtcctga cagccgtaat
    30181 ggtctgggtg atttggggcg tgatcgttaa tgcctggttt attccagaaa ttgccagcca
    30241 gttcttcacc atgggtctgg tgattggcat catcggcgtt gttttccgcc ttaacggcat
    30301 gacggttaat accatggctt catcctttac cgaaggggcg cgaatgatga tcgcccctgc
    30361 cctgctggtg ggtttcgcca aagggatttt gctgctggtt ggtaatggtg aagcaggtaa
    30421 tgccagcgtg ttaaatacta tcctcaacag cattgccaat gccattagcg gtctggacaa
    30481 cgcggtcgcg gcctggttta tgttgctctt ccaggctgta tttaatttct tcgtgacgtc
    30541 cggttctggt caggcggcat taactatgcc gttactggca ccgcttggcg atctggtcgg
    30601 tgttaaccgt caggttaccg tgctggcgtt ccagtttggt gatggcttca gccacatcat
    30661 ttacccaacc tcagcttcgt taatggcaac gctcggtgtg tgccgggtgg acttccgtaa
    30721 ctggctgaag gtgggcgcga ccctgcttgg actgctgttt attatgtcca gcgtcgtagt
    30781 gatcggcgct cagttgatgg gctaccacta aaaatgttaa gagccgcaat tgcggctctt
    30841 ttttcattct gccgtttcag tctctacggc ttcatttttg gcattgcgct tcatccacaa
    30901 cgccagcgct ttcagcgagt ctggagtgaa ctcatcgcag cgtgcggtga tttcttccgg
    30961 cgtcagccag caaacttcac tgacttcatc ttcctgtagg gcgaagggac cgtgagagac
    31021 gcaactgaac aacgcgcccc agacacggca atttttatct tcgaaataga actgcccgtg
    31081 ctcggcaaag gggacaccgg caatgcccaa ctcttcttcc gcttcgcgac gcgcggattc
    31141 cagcagttgc tcatcggcct gaactacacc gcctgcggtc gcatctaaca tgccgggtaa
    31201 aaagtctttt gtctcggtac gacgctggac cagaattttg cccatgccat catgcacgac
    31261 gatgtaagtt gcacgatgac gcagacactg tgcccgcatt tgttcccggc tggcttgtgc
    31321 aatgacttcg ttctcttcat tgacaatatc cacccattca gtacttgcca aacgacgctg
    31381 ttccattatc aggaaacctt ctttttctgg cgctcttacg gcgcattttg agttgtgggt
    31441 aaattacgga ttaatcgcga cctgcgcaat gatactttga tcattgagtg cgattacact
    31501 aagtacgtca ttatccagca tgccataact cgccggatta ccgcctttcg gaatactcac
    31561 cgagccgggg ttgaagtgga aaatctcccc ccgctgttct gccactggca gatgggtatg
    31621 accgtacacc agcacatcgt tctggtttaa agcaggtaga ttttccgggc caaaaagatg
    31681 accatgcgtc aaaaacagac gttgtttttc cagtaatacc tgttgccacg gcgcggttat
    31741 cgggaaatgc agcatcattt gatccacttc gctgtcgcag ttgccacgca cagcgataac
    31801 cttatgtgcc acttcattaa gccgttcagc aactttggct ggcgcgtaac cctccggtaa
    31861 agcattacgc gggccgtgat tcaacacgtc gccaaggatc accagccact gggcaccgct
    31921 ttggacaaac aactccacaa cacgttccgt cgccggtaac gacccatgaa tgtccgatgc
    31981 aaacatcagt ttcatcactc actcctcgtc gaaaaaacgt aagctatgat actggattct
    32041 gtcgttgcta tcagccagat tgcttcgccg aaagtgcaat aaaacgctgg acagacgctc
    32101 gctgccactg gaatgtcgca taatccacca ggcgttctgg cacctcatcg ccatgtagta
    32161 ccaggcggtt aatcattagc gccagatcag tatcagcaat gcaccattca ccaaataaat
    32221 tcggctgacc cagtgctaac aaatgttctg ccatcgcgaa cagtttctct gcactggctt
    32281 ttccctcggc cgttagtggc gcttttttcg cccccgcaaa gacaacatcc gtcggacgct
    32341 cttcgcggat gggcatcaga tcgctgcgca gccaggcctg aatctgtcgt gcacgcgcac
    32401 gattttctaa atcaagcgga taaatacgtt cccaggtcgg tggcgcaaat cgatcttcca
    32461 gatactccgc aatggcagaa gattcactca actcaaaatc atcgatttgt aataacggca
    32521 cacggcgtgt ctgaccgtaa ccttgccacg tcggctgcaa atgttcaccg ctgtcgaggt
    32581 cgatggtctt gatatgaaac gacaggcctt tttcctgcaa cgccacccag gcggataaca
    32641 cataagggga gaaaaagtgg gcatctgacc aaagcgtgat agcgggttta ctcattatgt
    32701 cctcggcaat aaagggggct ggctatcaaa ctatagagcc tttttgtcac tgtcacgtga
    32761 cgaatactca cgtaagtcgc cgcgctttct atacttgtgg tgttcagatg attaaacaac
    32821 cggagctgca atgatcgatc tctatttcgc cccgacaccc aatggtcaca aaattacgct
    32881 gtttctcgaa gaagcagagc ttgattatcg cttgattaag gtagacctgg ggaaaggcgg
    32941 tcagtttcgt ccggaatttt tgcgcatttc gcccaacaac aaaattccgg caattgttga
    33001 tcattctcct gccgatggcg gcgaaccgtt aagcctcttt gagtctggtg ccattttgtt
    33061 gtatctggct gagaaaacag gactcttttt gagtcatgaa acgcgtgagc gcgccgccac
    33121 attacagtgg ttattctggc aggtaggcgg actggggccg atgcttgggc aaaatcatca
    33181 ttttaatcac gcagcccccc aaaccattcc ttacgctatt gaacgttatc aggttgaaac
    33241 tcagcgtctt taccatgtac taaacaagcg gctggaaaac tcgccctggc tgggaggcga
    33301 gaactacagc attgcggata ttgcctgctg gccgtgggtt aatgcctgga ctcgcctgcg
    33361 aattgacctc gcaatgtatc cggcagtcaa gaactggcat gagcggatcc gttcgcgccc
    33421 tgccaccggg caggcactgc taaaagcaca actcggtgat gagcgttcgg atagttaaca
    33481 gaaacaggtt ctcgtgtatt atttcatcct aagtaaaaca acggagaacc tgcaatggca
    33541 caacctgccg ctattattcg tataaagaac cttcgtttgc gtacgtttat cggaattaag
    33601 gaagaagaaa ttaacaaccg tcaggatatt gttatcaatg tgacgatcca ctaccccgcc
    33661 gataaagcgc gcactagcga agatatcaac gatgcgctga attatcgcac cgtaacgaaa
    33721 aacattattc agcatgtaga gaataaccgt ttctctttgc tggaaaaatt aactcaggat
    33781 gtgctcgata tcgtacgtga acatcactgg gtgacgtatg ctgaagtgga gatcgataaa
    33841 ctgcacgcgc tgcgctacgc cgattcggta tccatgacct taagctggca gcgttaatcg
    33901 ccatatcggg aggctgcatg aacatagtga tcaccggagg gacgggatta attggtcgcc
    33961 atttgattcc gcgtttgctg gagctgggcc atcaaattac ggtagtgacg cgtaacccgc
    34021 agaaagccag ttccgttctc ggccctcggg tgacactatg gcaagggctt gccgatcaaa
    34081 gcaacctcaa cggcgttgat gcggtaatca acctggccgg agaaccgatt gctgataaac
    34141 gctggactca caagcaaaaa gagcgtctct gccaaagcca ctggaatatc acgcaaaaac
    34201 tggtcgattt gattaatgcc agcgacacgc cgccgtcggt actcatttcc ggctcggcaa
    34261 cgggctatta tggcgactta ggtgaagtgg tggttaccga agaggaaccg ccgcataacg
    34321 aatttactca taaactctgc gcccgctggg aagagattgc ctgccgggcg caaagtgaca
    34381 aaacgcgagt gtgcctgctg cgaaccggcg tggtactggc accggacggc ggtattctcg
    34441 gtaaaatgct gccaccgttt cgtcttggcc tgggcgggcc gattggttcc ggtcggcagt
    34501 atctggcctg gattcatatt gatgatatgg tcaacggcat tctctggctg ctggataacg
    34561 agctgcgcgg gccatttaat atggtttcgc cctacccggt acgcaatgaa caatttgccc
    34621 atgcgctcgg tcatgcgctg catcgcccgg ctattttgcg cgtccccaca accgccattc
    34681 ggctgttaat gggcgaatct tcagtactgg tattaggtgg acaacgcgcg ctgcctaaac
    34741 ggctggaaga agcgggtttt gcgtttcgct ggtacgattt agaagaggcg ctggcggatg
    34801 tcgttcgctg atgtggttta cagcaaacat ccgccagtta actccctgtg ttacaggatt
    34861 agtggctttg cgcgataaga tcgtctggtg aaagtcgggt caccatcata actaactctc
    34921 tgtctaaacc tctatccagc atctcctgag caatacgcag ggcttcttcg tgtttgccct
    34981 gcattgcgcc ttcttcacgt aatctgtcag caatggtcat cagtttctcc ttttcttgtg
    35041 gtgcgcgttc tgttatctca ccaataaatg cacggaaacg ctgggcatcc cctgtttgta
    35101 atacgtaatt aaacagggct tttagctgtc tgtcattagt gttccctgta actagcagcg
    35161 aaacaatttg gtcgactaat cccaacagat cgcgctgacg aatatgtttc tgaattaact
    35221 ccaacagcgc cattttgcgg tgttgcataa tctcgtcatc cggcaccacg gtaatatcca
    35281 ccaacggaaa cgctgatgaa tatattttgc gggctatagc aggctcggca aattcatcaa
    35341 gccagcagag tgaataagga taaggacttc tgcaaccatg ataaaacagc atcgggatca
    35401 ccaatggaag ctctttatag cccgcatcaa gatggttttg cattgccgca atggaataac
    35461 gcatcatgcg aaaagccatt aattcttccg gcttactttg gtgctctatc actacataaa
    35521 tataacccac tccctcctgc gttttcacag accacaagag gtcggaataa tattgccgca
    35581 ggtcttcatc aataaaactg tttggttcta gtttaagcgt cgttaaatca cacagtttgc
    35641 gcagcggcgc gggaagatga atattaataa aattccgcgc ggtgtctgga tggcgtaaaa
    35701 aagatttaaa taccgcatca tgcggcgtgg aagttgtcga tattgtcatg gcgttccgtc
    35761 acccctcaaa tttaagatga cgcgacaata accacgatta acacccaatg cagcaggcag
    35821 tttttatctt tggacagcga attcagaagt atttttagcg gactgcaact tttacatttc
    35881 agcggaagct gactcgcaaa ttccccttga aaatactccg gcagcgttag ccaccggaga
    35941 ggaaattatt tcagcgatcc cttaaggaac tgttgcagac gagggctttg cgggttgcca
    36001 aataactgct ccggcgcgcc ctcttcttct attttcccct gatggaggaa aatgacgtga
    36061 gtagaaacat ggcgagcaaa gcccatttcg tgagtcacta ccaccatggt tttcccctct
    36121 tctgccagtt gctgcataat acgtaacact tcgcctacca gttcaggatc gagcgccgag
    36181 gtaggttcat caaacagtaa aacttctggt tccatcgcca gcgcccgcgc gatagaaaca
    36241 cgctgttgct gaccgccgga aagatgcacc ggatatttcc cctgcgcacg ttcgtctatc
    36301 ccgactttcg ccagatactt caccgcccgc tcgcgcgctt cctgcttgct caggcccaac
    36361 acctgaatcg gcgcttccat gacgttttcc aacaccgtca tatggctcca gagattgaag
    36421 tgctggaata ccatcgtcag gcgtgtgcgc agtaagcgca gttgattttt atcggcgact
    36481 ttgagttgac cgtctttgtc gcgcaccaga ttgatcgtct ggccattgac cacgatcgat
    36541 ccttcactcg gtttttcgag gaagttaatg cagcgcagaa aggtactttt ccccgatccc
    36601 gacgatccga tgatgcttat tacatctccg gcattcgctt gcagtgagac ccctttcagc
    36661 acttcgtgtt cgccatagcg tttgtgcaaa tcgataacgt ttaatttatt ctcggacatc
    36721 gtattctcag tgcgttgaag aaggtttcac atgctgcaac cagcgttttt ccgctctgcg
    36781 aaagaggctg atcaggacat aagagatgat taaatagagc accgcggcaa tgccgaaggc
    36841 ggtaaaaggt tgatacgtgg cggcgttaat atcgcgggct attttcagca aatccggcac
    36901 agtggccgta aacgccagcg cagtagagtg cagcatcagg atcacttcgt tgctgtatgc
    36961 cggtaacgca atacgcagcg cagaaggcaa aataatgcag cgatacattt taaaggtcga
    37021 gaagccatag gctctggcgg cttcaatttc cccatgcgga accgaacgga ttgccccagc
    37081 aaaaatctcg gtagtgtaag cgcaggtgtt aagcgtcagc gccagcacgg tacagttcag
    37141 gccactgcgg aagaaagcgt taaggaattc ggttccctta acaatctcaa gcgtgtacat
    37201 gccggaatag aacaccagca actgaacata cagcggcgta ccgcgaaaaa tataggtaaa
    37261 taaccagatt ggaaactgga tgtatttatt actggagaca cgaccaatcg ccagaaacag
    37321 cgccaggact ccgcctatca ctaccgacaa aataagcagc cacagagtga tcgccacacc
    37381 agtaaagcga taaccgtcgg tccacagcag cggtttccag tattcatgta agatttcgat
    37441 cacaggtcag ccctcttcac acccacggag tagcggcgct caaggaacag cagcacacca
    37501 ttggaaacgg tggtgaaaac caggtaaatc acgccacaga cgatggcgaa atagaacggt
    37561 tcccaggtac ttttgcctgc cagctgcgtg gctttgacca catcttccag gccgagtaac
    37621 gaaaccagtg cggtagattt gaggatcacc tgccagttgt tgccaatgcc tggcagggcg
    37681 taacgcatca tcgccggaaa catgatcctc cgaaacactt gcccgcgagt aaaaccgaac
    37741 gccgtcgccg cctctatatg ccctttcggc actgccataa acgcgccacg gaacgtttcg
    37801 gtaaaataag caccgtagat aaaaccgaga gtgataatac cagcgaccat cggatcgata
    37861 tcaatctgcc cgacgcccat cgcctccgtc accatgttta gcgcaatctg caaaccatag
    37921 aaaatcagca gcatcaacac caagtccggc acgcctcgaa tcagcgtggt gtacccttcg
    37981 aaaatcagcc ccaaaagccg attttgcgag agcttaccgc cagcgccaat taaaccgatg
    38041 attacagcga gcactacaga gctgatagcc agctccagcg tgacgagcgc accctgtaaa
    38101 ataacacctg aaaacccata caacatgctg cctgtcctgt cgtgtgtggt gaattaccgc
    38161 ctcttgtctc cctccggtta cccgaaggga gcgggctttc tttcaccgat ggcgattaac
    38221 caccataaac atcaaaatcg aagtactttt tcgctaattt ctcgtaagta ccgtcagcgc
    38281 gcatttcggc aaaggctttg ttcagtgcct cgcgcagttc gttatcttct ttacgcaggc
    38341 ccatgccggt ccctacgcca aatagttttt catctttaac agacggacca ccgaatttgt
    38401 aatctttacc gacgggttgt ttgaggaaac cttcgctggc agcgacctca tcctggaacg
    38461 cggcatcaat acgtccggca gtcaggtcag aataaatgtt gtcctgcccc tgatacgaga
    38521 cgatttcaat gccttttggt gcccagtgtt cattaccgaa cgtctcctgg gtggtgccct
    38581 gcaatacgcc tacccgtttg cctttcagcg actcgactgt cggctgaatg tcagaatttt
    38641 tcgccaccac cagacgagaa tcggcagcgt acagtttgtc ggtgaaggca atttcttgct
    38701 gacgtttttc cgtaatggaa agcgatgaca tgatggcgtc aatcttcttc gcttttaagg
    38761 acgggattaa cgcatccagc gggttttcga caaacgtaca ttgcgtattg atgcgcttgc
    38821 acaactcctt cgccagctcg atatcgaaac caaccagttc gccttgtgaa ttttttgatt
    38881 caaatggcgc ataggtcggg tcggtaccga tgcggatgtt ttgcggaatt gcagcaagtg
    38941 ccgcagttgc gctggagaag gccagaacca gagagagcga tagcaccagt tttttcataa
    39001 tgtcctcaac tgacagtctt tttataggga atatttacag gtttgttaca cttatcgtgc
    39061 cataaaattg accatcaagg caaaatatcc cgccccgtta gacgtattat ttttcattaa
    39121 aaatgcttaa gtatgcattt caatgcacca cgatagtgca acaaacctgg cgtgcaccat
    39181 gatgatcatt ccatcaggta cagcttccca gcgacgtatc agtcaccgta gacattaaag
    39241 tcgaaatact ttttcgccat cttgtcgtag gtgccgtcct gacgcagctc gccaagcgcc
    39301 ttattgaagg cagccgtcag ttcagcatca tctttacgta gccctacacc ggtgccatca
    39361 ccgaagtatt ttttgtcttt tactgatgag ccagcaaagg cgaaatcttt accagcaggt
    39421 tgcttgagga atccttcgct ggcagcaact tcatcttgta acgcagcatc cagacgtcct
    39481 gcagccagat cggaatagac caaatcctgg ttggcatagg ccaccacatc cacgccttta
    39541 ctacgccagg tctcgttagc gtaagcttcc tgggttgatc cctgcagcac accaacatgt
    39601 ttacctttca gtgaatccag cgttggctga atcggtgaac ctttggcagc aatcaaacga
    39661 gaatctgcgg cgtacagctt gtcggagaag gcaatctcct gctgacgttt atcggtaatg
    39721 gaaagcgacg aaataatagc gtcgattttt ttcgctttca gtgaggggag cagcgcgtca
    39781 aagtcactgg caacccaggt acatttcacc tgcatccgtt tgcacatctc gttaccgaga
    39841 tcgatatcaa agccaacaaa atcaccttta gcatctttcg acgagaacgg cgcataggtg
    39901 gtatcggttc cgatacgtac cgtctccggt agcgccgcat agctggaagc cgctgcggag
    39961 agaccgacta ataaagacag agcgagaatc gacttcttca tacataaccc tcaagtgaaa
    40021 atggcttttt tatgttgtgt attgtgttgt gtgtttgcag gctctttcat gcaggtctta
    40081 tgccatcttg acgacaacag taacattcaa cgttaaatat gttaataaga cgttgcatta
    40141 ttgtcctgaa gttgaagata gcaggtatag cggttgaatc gcagcgtttc gattgaatgg
    40201 cagaaacaaa atgtcgagga tttgatcgcg gttgcaaaat tgccctgaaa cagggcaaca
    40261 gcggagttat gcgccctgcc agcgggcaaa gagatcttca ggaagggtta ttgcaaactg
    40321 gtcaagaaca cgattaaccg tctgatttat cacatcatca agggattgcg ggcgatgata
    40381 aaacgccgga acgggaggca taatcaccgc accgatttct gccgcctgag tcattaaacg
    40441 cagatggcct aagtgcaatg gtgtttcacg cacgcagagc accaacgggc gacgctcttt
    40501 cagcaccaca tctgccgcac gggtcagtaa gccatcagta tagctatgga caatgccgga
    40561 cagggttttg attgaacagg gtaaaatcac catccccagc gtctggaaag aaccggaaga
    40621 gatgctggcg gaaatatcgc gcgcatcgag cgtgacatca gctaatgcct gcacttcgcg
    40681 cagggaaaaa tccgtttcga gggataaggt ctggcgcgcc gcctggctca tcaccagatg
    40741 cgtttcgata tctgtgacat cgcgcagaac ctgtaataag cgcacgccat aaatcgcgcc
    40801 gctggcaccg ctgatgccta caatgagtcg tttcataaat agttgccctg tcagacttgc
    40861 aggcagactt tgcagtattt cggcgggagt tgcaagtcag ggtgccagac cggcaccctc
    40921 agcgaaggca tcatccttcg ttatgcattt cgagattttc cacttcgttc tgacgttgca
    40981 ctgctttggc gtcatcatta cgtaacgtat cgaggaaatc gaggtagccc tgatcaacat
    41041 ctttggtgac gtagacgccg ttgaacaccg agcattcaaa ctgctggata tccggatttt
    41101 cagcgcgaac ggcgtcgatc agatcgttca gatcctggaa aatcaacccg tcagcaccga
    41161 tgatctggcg aatttcatca acttcgcgac cgtgagcgat cagttccgtg gcgctcggca
    41221 tatcaatacc ataaacgttc gggaagcgaa tttccggtgc cgcagaagcg aggtacactt
    41281 tcttcgctcc ggcttcgcgt gccatctcga taatctgctc agaagtggtg ccacggacga
    41341 tggagtcgtc gaccagcagg acgtttttat cgcggaactc ggcgcggttg gcattcagtt
    41401 tacggcgcac ggacttacga cgcagctgct ggcccggcat gataaaggtg cggccaacat
    41461 agcggttttt aacgaagccc tggcggtacg gtttgcccag aatacgagca atttccagcg
    41521 cgatatcaca cgaggtttct gggatcggga tcaccacgtc gatatccaga tcttcccatt
    41581 cgcgggcaat tttctcgccc agtttcgtgc ccatattcac acgcgcgctg taaacggaaa
    41641 ttttgtcgat aaacgagtcc gggcgggcaa agtatacata ctcaaacagg cacggattgc
    41701 tgaccggatt gtcagcacat tgacgggtaa acaactgccc ttcttcagtg atgtaaatcg
    41761 cttcgcccgg cgcgacgtca cgcaggaaat caaagcccag cgtatcgagc gctacgcttt
    41821 cggaagcgac catatattct gtacggttct cgtcaatatc acgttttccc agtaccagcg
    41881 gacgaatccc gtttggatcg cggaaagcaa ccataccgtg gccgataatc atcgccacac
    41941 aggcatacgc gccgcggatt aagcggtttg tggcagcaat ggcagcgaaa atattgtcgg
    42001 cttccagcgg gtagtggcgg aagttgtcca gctcgctggc gaagatatta agcagaattt
    42061 ccgagtcgga agtggtgttg atgtggcggc gtttttcttc aaacagtttt ttacgcaact
    42121 cgtgagcgtt ggtcagattg ccgttgtggg caagcgtaat gccatacggg gagttaacgt
    42181 aaaacggctg cgcttcagag gcgctggagc tgccagccgt agggtaacgc acatgaccaa
    42241 tgcccatatt gccctgcaaa cgctgcatat ggcgagcttc aaatacatcg ctcaccagcc
    42301 cgttcgcttt acgcaaacgg aagcagttat tggcatctat ggtgatgatg ccggcggcat
    42361 cctgaccgcg atgctgaagc accgttaagg catcataaat cgactggtta accggcataa
    42421 caccggcgat accgacaata ccgcacatac gtctttttcc tcgttaagcc acatctcaga
    42481 gcacttacgc tctgggcaag aaacttgacg agctttgcag ataatcaaaa aaccatctga
    42541 tgataaaact gaattgcggg atcagctgtg atttgctcca gtcttcactt ttcgacaccc
    42601 cggtaaagga gtcgagaaag aagagaatgg cggcaacaat caacacaccg cgcaacgcac
    42661 cgaaacagac gcccagcacc cgatcggtgc ctgacaaccc cgttttctcc accaactggc
    42721 ctatcacgaa gttcacgata gcaccaacga tcagggtagc gataaacagt accgcgatgg
    42781 caatcccatt tcgaaccagt tcgtcttcaa agcccgtaaa ccagactgac aggtaagtgt
    42841 agtaatgact ggcaacaaag aaagcacaac cccatgtcac cagcgataac gcttcacgaa
    42901 caaagccgcg gatcaggcta accagagagg aaaaagcaat caccgcgatt atggcgtaat
    42961 caatccagac catatgtgtc ccacgatttt acgccctgtc atcctgttcg gggcgcattc
    43021 taacagaaaa agaaaacgtt tgcgtaggga tttccttccc gcgcatcaat aaaaatggcg
    43081 ctgaaaaaat attcaacgcc atcgactttt tatgcctttg cggcatcgtg caatgcgtgt
    43141 cggatgcggc gtaaacgcct tatccgacct acggttctaa cactgcccaa gcctgataag
    43201 acgcgccagc gtcgcatcag gcaagactgt gttaattcgg cgtatagccc attaccacgc
    43261 cacttaagcc agaaagttgc ttcaactcac ccagcgaacc tttcagctta tctttcgagg
    43321 catccggccc aaccagaata cgggtaattt taccctgcac tggcgtggat ggcgacgtat
    43381 aaacccgata accggcaccg cgcagcttac cgacaatctc attcacttta tcggcatttt
    43441 tcagcgcacc cagttgcaca acataggctt tacccgttgg cgctgctttt tcttccacga
    43501 ccggcttcgg ttctggcttc ggcgcaggtg gtgcttcaac ctttggttta ggcggctcca
    43561 ccggtttcgg cttcggtggg gcgaccggtg caggttccgg ttcaaactcg gtgttattgg
    43621 ctgcaatagt ggccggatcg agcgacggtg ctgcggcatc acctgcccgc acctcttccg
    43681 ctgcgccttc cggcggctgc gtcggcaacg cctgggtggc ggctggcatc atatcaggct
    43741 catcacgatc gcccgctttc ggcaccagcg ggatagccgc gaactcatcc tgataatgtt
    43801 ttttctgccc gtccagcagc cctggaagta caatcacccc cagcgccacc agcacgatcg
    43861 tgcccactaa ccgattctga aacttacttg ccaccgcttc tcctcgcgtc aatcacttcc
    43921 atgacatgtg cgaccgtgtg gaaagaacca cacaccagca cggtgtcttc cgctttagcg
    43981 tccgccattg cggcatccca tgcctgcgca acgctatcaa atgatttgcc gttacccaaa
    44041 tgctcaagca gttgttctgc cgtggcaccg cgcggccctt ccagtggcgc acaataccag
    44101 tcatcaacca cgcttttcaa ccaggccaga gttccggcaa tatctttatc atgtagcata
    44161 ccgataaccg ccagcacgcg cccgtttttc ggtagcgctt tcatacgccc ggtgagatat
    44221 tccgccgcat gtggattatg cgcgacatca aaaataacgc gtggcgactc gctcacaatc
    44281 tggaaacgtc ccggcaaaat tgcgctggca atcccgtcgc gaatggcatt ttcactgact
    44341 tccagcccgc tggcacgcag tgccgccagc gccgttgcgg cattcggttg cgggacaagc
    44401 ggcaacggca gattttccag cgtgccgtga gcatcgctaa acgcccagtc atgatcggtg
    44461 acggaatagt tccactcaac gccccgacgt tgtaacagtg cacctttttc ctgcgccaca
    44521 tcagcaatgg tagaaggcat ttccggctca ccgacaattg ccggtttttc gctgcggaag
    44581 atgcctgctt tctcgcgacc aatactttcg cgatctggac ccagccagtc ggtatgatcc
    44641 agcgcaatac tggttactac cgcggcatcg gcatcgacaa tattggttgc gtccagacga
    44701 ccgcccagcc ctacttccag aatcaccacg tcaagttgtg cctgcttgaa cagccacaac
    44761 gccgacagcg taccgtactc gaaataggtc agggaaatat caccgcgtgc cgattcaatc
    44821 tccgcaaaag aggcggtgtg ggccgattcc ggcaattccc ggccctgcac acgtacgcgc
    44881 tcggtataac gcaccagatg aggcgaactg tagacgccca ctttgtaccc tgccgccatc
    44941 agaatcgact ccagcgtacg gcaggtggtg cctttgccat tcgtacccgc aacggtaaac
    45001 acaaatggcg ctggtttcag gacgccaaga cgcgccgcga cctggctcac gcgctcaagg
    45061 ccgagctcga tagttttact gtgcaggttt tccagataag aaagccacga agccagaggc
    45121 gacgcggctt gaggagtgcg tttgataatc atggtatccg ctgattcgtt acggtgagaa
    45181 tagcaaaagg gcagagccag tggccctgcc cttatcagtt atcaggcctc aggttcctga
    45241 tccggtaccg ggggtaccac tacgccttca cgcggcgctt caggattcgg cgctggcaga
    45301 ttcatcaact tcgccagaat gctcgccagt ttcaggcgca tttccggacg acggacgatc
    45361 atgtcgatcg cgcctttctc gatcaggaat tcactgcgct ggaatctagg cggcagtttt
    45421 tcgcgaacgg tctgttcgat aacacgcgga ccggcaaagc cgattaacgc tttaggttca
    45481 gcgatgttga gatcgcccag catggcgaaa cttgcagaaa caccgcccat cgtcgggtcg
    45541 gtcagcacgg agatgtacgg caagccgcgc tcctgcattt ttgccagtgc cgcagaggtt
    45601 ttcgccatct gcatcagcga catcagtgct tcctgcatac gtgcgccacc agaggcggag
    45661 aagcagatca gcgggcagtt atcttcctgc gcctgctcaa cggcacgcac gaaacgtgca
    45721 cccacaacag accccattga accgcccata aaggcgaact cgaatgccgc agcgacaacc
    45781 ggcattccat acagagtgcc tttcatcacc accagcgcat ctttttcgcc ggtttctttc
    45841 tgcgcagatg ccagacggtc tttatacttc ttggagtcac gaaacttcag ctcatctttc
    45901 ggctcaagct cgctacccag ctccacaagg cttccttcat ctaacaggct atgcaggcga
    45961 ttacgcgctg tcatacgcat gtgatggtca cacttcggac agacctcaag attacgttcc
    46021 agctcagcgc ggtataaaac ctgaccgcag ctatcacact tagtccacac cccttcagga
    46081 atgctcgcct tgcgggtggg agtaatgttg cttttaattc gttcaatcca gctcattagg
    46141 gacctttctg tctgaacctg gttcgatgcc agttttatct ttggggacgc ataatgccat
    46201 ttttgccccc aacagaccat gaatgttgca cattaaaaca taacagcccg aaactttgga
    46261 taaaaaagtg gtcgaaccgc ggagttactt tttattttgc ggcgcgtgcc gcagcgcgtt
    46321 tgtgacggat tatttcgatg acgcccggca aaatagaaac cacaataatc ccgacgatca
    46381 gcagcttaag gttatcctga accatcggga ttgtaccgaa gaaatagcct gcgtaggtaa
    46441 aaagcagtac ccacaacagt gcgccgatca cgttataagc agcgaaatga cggtacgaca
    46501 tgtggcccat tcccgcaaca aacggggcga acgttctgac gatcggcaca aaacgggcga
    46561 gaataatcgt tttgccgcca tgtttctcat aaaactgatg ggttttgtcg agataactgc
    46621 gacggaaaat tttcgaattg ggattactga ataacttttc accgaacaac cgcccgatag
    46681 tgtaattgac cgcgtcaccc acaatcgcgg cgatcagcat cagcactacc atcatatgga
    46741 cgttgagatc gttggtttcc agtgatgcca acgctccggc aacaaacagc aatgaatcac
    46801 ccggtaaaaa cggcattacc accagaccgg tttcacagaa caaaattaag aacaaaatgg
    46861 cataaaccca gacgccgtac tccgcgacta gctctgccag atgcacgtca atgtgcagga
    46921 taaaatcgat gaggaaataa atcaggtcca tatttgccta tgccttgtac tcgtcatctt
    46981 tcaggctgta actacgttgg ctacagcctg aaatgttccg agcattattc tcgttagtcc
    47041 gccagaaata gcgggcccat tggcggtttt ggaagatcat accggtcagg gtaatccacc
    47101 gcgaccagat acagcccttc cgcttttgcc gttgctgccg ccagcgttct gtcctttgcc
    47161 gccagcaact ctgctatcca gctctccggc tggttgtggg caccgacttc catcaggctg
    47221 ccgacaatat tcctgaccat atgatgtaca aaggcattcg ctttgatatc aaccaccaca
    47281 taaggaccgt gacgcgtgac attaatatgc ataacgttgc gccacggggt tcgggactgg
    47341 cactgcaccg cacggaacga ggtgaaatca ttctcgccca gcaagcattg cgcagcccga
    47401 tgcatccgtt cagcgtccag cggttcgtaa aaatgggtta cccctttact cagtaccgcc
    47461 gggcgcagcc gatgattgta gatgatgtag cgataacggc gagccgtggc gctaaatcgg
    47521 gcatgaaaat catcaggtac agctttaacc caacgcacgg cgatgtcacc aggtaaattc
    47581 gcatttacgc ccaaggtcca cgccgcgtct ttgcgctgcg cggttgtttc gaaatgcacc
    47641 acttgcccgg taccgtgtac ccctgcgtca gtacgcccgg cgcagaagac ggtgatgggt
    47701 tcgttcgcca cctgggagag cgccttttcc agcttctcct gcacactgcg gacttcattc
    47761 tgccgttgcc agccgtaata cttactgccg tcgtactcaa tgcccagcgc aattttataa
    47821 actggcagtt gttgctggtc ggacattagt acagatactc ctgcaccagt ttctcggcga
    47881 tttttactgc catcagcgcg ccgccaaagc gaacgttatc ggccaccgac cagagctgga
    47941 cttgctccgg cataccgtag tcattacgca cgcagccaac agaaagatgc ggcgtacccg
    48001 aagcatcccc tacctgagtc gggaattcgt tctcttcaga gagcacaata tcttcgccct
    48061 gagcaaacgc atcacgcgct tcttctgccg ccagcggacg cagtgcttca aagttgacca
    48121 tctgggcatg accgtagaat accggtgcct ggacgacgct tgccgaaatc atcagccctt
    48181 cgtcctgcag gattttgcgt acttcgtcaa cgatacgacg ttcttcacgc acgctacctt
    48241 cgctatccgg caacaacggc agcatattga acgccagttg acgaccgaag aaatcttctt
    48301 cgtcaatcgg aatgccgttg agcaatttcg cactctgccc cgctaacgca tcgaccgctt
    48361 ttttgccctg ggcggaggct gaaatcaggc tggtaacact gatacgcgac aaaccgccct
    48421 gatcgattaa cggtttcagt gccgccagca gctggctggt cagactgtct ggtacggcga
    48481 tgacattccg gttgcggtaa tcggtcagta caaacgggtt tacttccggc accaccagcg
    48541 gtacgtcggg ttcgagagca aacagtccgc tgctgtcgat caccaggcaa cctgagttgg
    48601 tcgcttcttc aacccaggca gcggtagctt ctttgcctgc gacaaaaaat gccagctgcg
    48661 cctgcgtcca gtcgaattca gcggcatcct gcacggtgat tgttttacca ccaaagcgca
    48721 gttgttcgcc tgcgctttcg ttacgtgcca gtgcataaat ttccccaacc gggaactgac
    48781 gttcagccag cgtttcaagc agggcttcgc ccatagcgcc agttgcgccc aggacggcaa
    48841 tgttccagcc ttcagacatg gtggtttact ccagaaatag caaagctccc tgccaatgtt
    48901 acagcagaga gcatgaagaa gagattaacg tgccggatga tgaacggcgt taaaacccag
    48961 tttacacagc aatgatgccg cactggcgtc atcacaaatt acatacagag atgaccattc
    49021 acggcgctca agatagtttt tgcgcagttt atcgaactca cccggtatcc cggcgacttt
    49081 acgcagcggt gcgtcatcgc ggcgcacatc atacaccaaa tgcaccagcc ttttcagcgt
    49141 cggttgatcg agcggaccat gcagcgtaat gcggccaaac tcaggcgcgg gcagcaacgt
    49201 atccagcgca acgtgttgtt catgcccaat aaacttgcta taggcttcaa acacttgcgt
    49261 cgtgccacgc gctttaccct ccagggtata gcctgcgata tgcggcgtgc cgatatccac
    49321 ttttgtcagc aattccacat taagttccgg ctcgccttcc cagacatcca gcaccacgct
    49381 taacttctgg ccttcattca ggcaggtcag cagcgcggta ttatcgacga ctgcgccacg
    49441 gcaggcgtta atcagaatcg ctccgggctt caggctacgg atcagttttt catccgccag
    49501 atgtagcgtt ttgtacggac catctttaaa gagtggcgta tggaaagtca gaatatccgc
    49561 gcgctggact aactcatcca gcgagcggaa atcaccctca tccccacggt cggcgcgagg
    49621 cggatcgcaa agtaaggttt taatccctaa cgcttccagt cgcgcctgca atcgacgtcc
    49681 aacgttacca acgcccacga tccccaccgt acggtcgtgc agtgaaaatc catcgcgttc
    49741 ggcaagcatc agcagggagg agaaaacata ttccaccacc gcaatcgcat tacagccagg
    49801 tgcagcggaa aaaccaattc ccgcctgctt caaccatgct tcatcgacat ggtcggtccc
    49861 cgcagtggca gttccaacaa atttaatggg ttttcctgcc agcaaagatt cattcacttt
    49921 cgtgaccgaa cgcaccatca gcgcatccgc gtctgccagt tgagcgacgg ggattggacg
    49981 cccgggaacc gcgatcacct cacccaaacg gctaaataag tcgcgggcat aaggcatatt
    50041 ttcatcaaca aggattttca cgtttgtgtt acctgtatga gacgagagtt aaccggacaa
    50101 gtgtgccata atctcgcggc caggcatact tgcgaagatt tcaggtataa ggatacgtaa
    50161 tgatacaacc tatttccggc cctcctcctg ggcagccacc aggtcaggga gataacctgc
    50221 cgtctggcgc gggcaatcag cctttatcca gtcagcaacg tacttcgctg gaaagcttaa
    50281 tgacgaaagt gacctcactg acgcaacaac aaagagcaga actgtgggcg ggtatcaggc
    50341 acgatattgg tctgtcggga gattcaccgc tgctttcgcg tcacttccct gccgctgagc
    50401 ataatctggc gcaacgtctg ctggccgcgc aaaaaagcca ttctgcccgc cagcttttag
    50461 cgcaattagg ggagtattta cgtctgggga ataatcgtca ggcggtcacg gattatatcc
    50521 gtcataactt tggtcagacg ccgctgaatc agctctcacc ggagcaatta aaaaccattc
    50581 tcaccctgtt gcaggaaggg aagatggtta ttccgcaacc acagcagcgc gaggcgaccg
    50641 accgtccttt attaccggcg gagcacaatg cgctaaaaca gctggtgacc aaacttgcgg
    50701 cggcaacggg ggaacccagc aaacagatct ggcaatcgat gctggaactt tccggggtga
    50761 aagatggcga gttaattcca gcgaaactgt ttaaccatct ggtgacctgg ctacaggcgc
    50821 gtcagacgct aagccagcaa aatacgccga cgctggaatc actacagatg gcgctaaaac
    50881 aacctttaga tgccagtgaa ctggcggcgt tatcggcata tatccagcaa aaatatggcc
    50941 tttctgcgca atcatcgctt tcttctgccc aggccgagga tattcttaat cagctttatc
    51001 aacggcgggt taaagggatt gatccgcgtg atatgcaacc gctgcttaat ccttttccac
    51061 cgatgatgga cacgttgcaa aatatgccaa cgcgtcccgc gctgtggata ctgttagtcg
    51121 cgattatcct gatgctggtc tggctggttc gttaaccccg acgaaatgac agtatcgtga
    51181 caataatacc cagcaccgca gagatcgccc cggcaagaaa taccgaagag taaccaaacg
    51241 tggtcgccag cattcccgcc agcggcccgg agacgccgag ggcgatatcc tgaaacgcgg
    51301 cgtaaccgcc cagtgcggtg ccgcgaactt gtgaggggac gcgtttaacc acctccacgc
    51361 ccagcgcagg aaagataagc gaacatccgg ctccggttaa cgccgcgccc gctaatgcga
    51421 cccatgcacc tggggcttgc cagagcagca acaagcccac cgtttctaca agcagagaga
    51481 caatcgccac tttcacgccg ccaaaacggt ccggcatcca gccaaacatg acgcgcatca
    51541 cgacaaatgc gccgccaaac gcggtaagag taaagcccgc catcgcccat cctttgctgg
    51601 caaagtagag cgaaacgaaa gtcccgataa ccgcaaaacc aacgccttgt agtgccagcc
    51661 ctaaccctgg tttccagata agcccgacaa cgctccacag cgatggacgt tctcccgcca
    51721 gggccggtac tttgcgcact gtgccgttac aggcccacgc cagtaagggt aataccattg
    51781 tggtgatcgc cagtgcggca aaaccgtaat ggctatgaat caacaggcca agcggcgcac
    51841 caacagcgag ggcaccgtaa atcgccattc cgttccatga catcactttg ccagagtgtt
    51901 ttggccctac gatgcctaac ccccaggtca gagcgcctgt cagtaactgg ctttcaccaa
    51961 acccaagaat caaacgcccg atgaccaaca gggcaaattt gaacggtgcg gagacaggca
    52021 aaatcgccgc cagcagcaac gcgccgccag ccagaccaca agctaacatc ccctgaagcg
    52081 ccgaacgttt tgcgccatat tgatcggcca gtcgcccggc gtaaccacgc gtcagcaccg
    52141 tagccagaaa ctgaatcccg acggcaatgc cgaccatggt attgccatag cccagatcat
    52201 gatgaacaaa cagcgggata accggcaacg gcaaccctac ggtcatgtag gtgagaaaaa
    52261 ccgcaaaagc gatgcggaag agcgaaaaac tggcagaaga tcgtgtttcg gtttggctta
    52321 cagcagtcat gcattactcc agaatgcagc gcaaggcgag gagtatcccc gtctcatctc
    52381 tctggtttca gggttacggt gcgttggcag gatttaacgc gtacgtcttt tcagaaggaa
    52441 atcgacaaag cgggaagttt gcctagaact ggtggcgatt gtcaatgatg tgaaaaaggg
    52501 aaccatcagg ttcccttttg cgttagtgcc ggaggccgca ttggcgcgta acgtcggatg
    52561 cgacgctggc gcgtcttatc cgacctactc cggcctactg cgaattaatc tttcagcttg
    52621 cgcattacca gcgtggcgtt ggtgccgccg aagccgaagc tgttagacat aacagtggtc
    52681 agttcgcgat cggtcgtttc ggtcacgatg ttcagacccg cagcctgctc gtccagctct
    52741 tcaatgttga tgctcggggc gataaagccg tgttccagca tcagcagaga gtagatagct
    52801 tcctgcacac ccgcagcgcc cagtgagtga ccggtcatgg ctttggttgc agaaatcgcc
    52861 gggctcttat cgccgaacac ttcacggata gctgccagct ctttcacgtc gccaaccgga
    52921 gtcgaagtac cgtgagagtt caggtaatcg attggggtat caacgccatg catcgccatc
    52981 tgcatacaac gtactgcgcc ttcgccagac ggagcaacca tgtctgcacc atcagaggtt
    53041 gcgccgtagc caacgatttc agcatagatg taagcaccac gcgccagcgc gtgttccagc
    53101 tcttcaacca ctaccatacc gccgccgcca gcgataacga aaccgtcacg gtgagcgtcg
    53161 taagtacggg aggctttttc cggggtgtcg ttgtatttag tagacagcgc acccatcgcg
    53221 tcgaattcgc aggccatttc ccagcacagc tcttcgccgc cgccagcaaa cacgatgtcc
    53281 tgtttgccca gttggatctg ctctactgcg ttaccgatac agtgtgcgga agtcgcacac
    53341 gcggagctga tggagtagtt aacgccatga attttaaacg gcgtggcgag acaggcagaa
    53401 acgccggatg ccatcgcttt agtgaccaca tacgggccaa ccgctttcag gccgcgcggg
    53461 ccgcgcattg cgtcagcgcc gaacacctgg aaacgcgggg aaccgccgcc ggaacctgca
    53521 atcaggccaa cgcgcgggtt attctggtaa gcttccggag agaggcccgc atcagcgatt
    53581 gcctgctcca tagaaaggaa tgcataaatg gatgcgtcgc tcataaagcg cacaactttg
    53641 cggtcaatga ggccagtgat atccagtttt atgttgcccc agacgtggct acgcatgccg
    53701 gaatccttca gctcctgaga gaaagtgatc cctgaacgtc cttcacgcag agatgccagg
    53761 acttcctgct ggttattacc gatgctggaa acaatgccca ggccagtaat cactgcacgt
    53821 ttcattcaat acctctgtaa gtcgcacata gagtaagttt cgaatgcaca atagcgtaca
    53881 cttgtacgcc gaacaagtcc gatcagccat ttaatagaga aatttgcgca gccttacaca
    53941 catcgctaag atcgagccac cgcctgtaag acgagtaact tacgtgaaac actactccat
    54001 acaacctgcc aacctcgaat ttaatgctga gggtacacct gtttcccgag attttgacga
    54061 tgtctatttt tccaacgata acgggctgga agagacgcgt tatgtttttc tgggaggtaa
    54121 ccaattagag gtacgctttc ctgagcatcc acatcctctg tttgtggtag cagagagcgg
    54181 cttcggcacc ggattaaact tcctgacgct atggcaggca tttgatcagt ttcgcgaagc
    54241 gcatccgcaa gcgcaattac aacgcttaca tttcattagt tttgagaaat ttcccctcac
    54301 ccgtgcggat ttagccttag cgcatcaaca ctggccggaa ctggctccgt gggcagaaca
    54361 acttcaggcg cagtggccaa tgcccttgcc cggttgccat cgtttattgc tcgatgaagg
    54421 ccgcgtgacg ctggatttat ggtttggcga tattaacgaa ctgaccagcc aactggacga
    54481 ttcgctaaat caaaaagtag atgcctggtt tctggacggc tttgcgccag ctaaaaaccc
    54541 ggatatgtgg acgcaaaatc tgtttaacgc catggcaagg ttggcgcgtc caggcggcac
    54601 gctggcgaca tttacgtctg ccggttttgt ccgccgcggt ttgcaggaag ccggattcac
    54661 gatgcaaaaa cgtaagggct ttgggcgcaa acgggaaatg ctttgtgggg tgatggaaca
    54721 gacattacca ctcccctgct ccacgccgtg gtttaatcgt acgggcagca gcaaacggga
    54781 agtagcgatt atcggcggtg gtattgccag cgcgttgttg tcgctggcgt tattacggcg
    54841 cggctggcag gtagcgcttt attgcgcgga tgaagccccc gcgctgggtg cttccggcaa
    54901 tcgccagggg gcgctgtatc cgttattaag caaacacgat gaggcgctaa accgcttttt
    54961 ctctaatgcg tttacttttg ctcgtcggtt ttacgaccaa ttacccgtta aatttgatca
    55021 tgactggtgc ggcgtcacgc agttaggctg ggatgagaaa agccagcata aaatcgcaca
    55081 gatgttgtca atggatttac ccgcagaact ggctgtagcc gttgaggcaa atgcggttga
    55141 acaaattacg ggcgttgcga caaattgcag cggcattact tatccgcaag gtggttggct
    55201 gtgcccagca gaactgaccc gtaatgtgct ggaactggcg caacagcagg gtttgcagat
    55261 ttattatcaa tatcagttac agaatttatc ccgtaaggat gactgttggt tgttgaattt
    55321 tgcaggagat cagcaagcaa cacacagcgt agtggtactg gcgaacgggc atcaaatcag
    55381 ccgtttcagc caaacgtcga ctctcccggt gtattcggtt gccgggcagg tcagccatat
    55441 tccgacaacg ccggaactgg cagagctgaa gcaggtgctg tgctatgacg gttatctcac
    55501 gccacaaaat ccggcgaatc aacatcattg tattggtgcc agttatcatc gcggcagcga
    55561 agatacggcg tacagtgagg acgatcagca gcagaatcgc cagcggttga ttgattgttt
    55621 cccgcaggca cagtgggcaa aagaggttga tgtcagtgat aaagaggcgc gctgcggtgt
    55681 gcgttgtgcc acccgcgatc atctgccaat ggtaggcaat gttcccgatt atgaggcaac
    55741 actcgtggaa tatgcgtcgt tggcggagca aaaagatgag gcggtaagcg cgccggtttt
    55801 tgacgatctc tttatgtttg cggctttagg ttctcgcggt ttgtgttctg ccccgctgtg
    55861 tgccgagatt ctggcggcac agatgagcga cgaaccgatt ccgatggatg ccagtacgct
    55921 ggcggcgtta aacccgaatc ggttatgggt gcggaaattg ttgaagggta aagcggttaa
    55981 ggcgcggtaa tctgctctcg cagtgtttgc cggatgcggc gtgaacgcct tatccaacct
    56041 acgtgtgacg tgtaggcctg ataagacgcg ataagcgtcg catcaggcat ggtgctccag
    56101 acgccgcaac ttacagctgt gacgcctgct gaaacaggtt ttcccacata tcggtcacca
    56161 gcgcctggtc acgcggcgac aattcaccgg caccaatggc tttttccaga ctctggctaa
    56221 cggtcgtatg caccgcctga gcggagtggt catcaccact ttccagttct gcgatggcta
    56281 atgtcaggtg gccacgcaaa tacccactgg caaacaactc atcatcactg gcatggtcaa
    56341 ccatgccgtc gattaatgcc agaatgcgtg attcaaactc cgcgatcatc ttctttcctc
    56401 attgtaaaac gtggcgctgg cttcagttga gcgcttccgg ccacgggaac tgttccgccg
    56461 taagttccgg cgtgtgataa taattttgta gtgctttaat gaagcgtgcc gggcgttccg
    56521 gaatgccatt tgtcagataa tccatcacct gcgcatgtac ccgccgttga aacaccacac
    56581 gatccggttc gaaatcccct tccagattgt cgcaactgac gttaaacgga tatccagccg
    56641 ccacgcagaa taaccagtcg aacgcctgcg gcttcacttc aacatcttca aactggcttt
    56701 gcgtctgggc atcgcgtccg tccgggcaat accagtagcc gaaatcaacc agctcacggc
    56761 gcgcttttcc ggcaatacac cagtgcgaaa tctcatgaat tgcgctggca taaaagccat
    56821 gagcaaagac gattcggtta tacggtactt ccgcatcagc aggaagatag atcggttcgt
    56881 cgtcgccttt aatcagacgg gtattaaatt catcggcaaa gcagctatta aaaatttcaa
    56941 tcaactgctc gtagtggtgt gtactgttca ttagttcatc cccaaccagt ggaggatctc
    57001 ctgtccgtgg ctatcgtaaa gtaatttggc actcatcacc gccgagacga taacaatcat
    57061 cgggcggatc agcttttgtc ctttgctcaa caccaaccgt gaacccatgc gcgcgccgag
    57121 gaactgcccc accagcatga caaagcccgt cgcccaaatc actttgccgc cgagaataaa
    57181 cagcagcaga ccgccaatgt ttgacgttgc gttgagtaat ttggcgtgag ccgtggcttt
    57241 ggcgaggttg aatccgcaca gcataacgaa ggccagcgcg taaaacgatc cggcagccgg
    57301 gccaaagaat ccgtcataaa aaccgacgca gccaccggca attaacgcga agggtaaacc
    57361 gtacatccgg cgctggcgat cttcttcacc cagctttggc atcagcaaga aatagaggcc
    57421 gatacaaatc accagaatgg gcaaaatctg ccgtaagaca tcagcctgaa cgtactgtac
    57481 caacagtgcg ccgctcattg agccgacaaa ggtcatggca atattgagtt tctgatcgct
    57541 taaactaacc actttgcggc gaataaagta gatagtggcg gaaatagagc cgccgcaggc
    57601 ttgcagttta ttggttgcca gcgcgttagc gggagacatt cccgcagcca tcaatgccgg
    57661 aatggtgagt aacccaccgc caccggcaat cgagtcgata aatcccgcca gcatggcgac
    57721 aaaaaagaga actcccagca acagcggaga aaccataaac aggctattaa acgtttccat
    57781 tagatcacgt gctcatccag tagcgcctgg caggaaggcg gcaacggagg cggtgtcttc
    57841 ttctcaggct ttgttgttcc cggttttgga ggttcaaacc agctttgcag ttctgccccg
    57901 caaccatcgc ctgatggcgg taaaggttga tcttcacact ccagactatc ggcagggcaa
    57961 cgtaatcgta catgcatatg cgcgcgatgc tggaaccagg gtcgcacttt gcgcaaccag
    58021 tcgcgatcgg tgcccgcatc aaggcaaagt tgttgtttaa tcgccggatt aacaaaaatg
    58081 cgcgtgacgt ctttgtcctg ggcggcgagt ttgatcaagc tgaaaatttc tggcttccac
    58141 agcgtggaga caacgtgttt accgtcgcgg gaaactaagt ccagtgcttg cgggcgcaag
    58201 agctgcgcgg aggtccagcg agttttcggc agttgcagaa agatatcgac atccagtccg
    58261 gtctggtggc tggcatgacc gccgttgaaa cgcccaccag cgggcatccc catatcgcca
    58321 atcagcaccg tacccatgcc cagattgctc acctggctac tcagacgctg gataaacatc
    58381 accagatccg ggtgaccgaa atagcgacgc tgatcggtac gcatgacctg ataatgttcg
    58441 gactgtatcg gcaacgtgtc agcgccgaca atacagccat tagaaaaact gcctatcgat
    58501 tgtgcgctac ccggcacagg ttgggttatt ttttgccacg gcgtcgctgc caggctagcg
    58561 ctactggcaa gcagagccag cagcgcaatc gcggttttgt tcatttttta ccagcgtgga
    58621 atatcagtct tcacatcggc attttgcgcc cgctgccgta acaggtgatc cattaaaacg
    58681 atcgccagca tcgcttcagc gatcggcact gcacggatcc cgacacaggg atcgtgacgg
    58741 cctttggtga tcatctcaac ttcttcgcca aagcggttaa tagtgcgccc cggcacggta
    58801 atgctggagg tcggtttcag cgccatatga gcaatgattt gctgcccgct gctgataccg
    58861 ccgaaaatgc cgcccgcatg gttgctctgg aaaccctctt tggtgatttc gtcgcggttc
    58921 tggctgccgc gcagcgccac tacgtcaaaa ccgtcgccaa tttccacgcc tttcaccgcg
    58981 ttgatgctca tcagcgcatg ggcgatgtcg gcatccaggc ggtcaaagac cggctcgcca
    59041 agtccggcgg ggacaccact ggcaacaacg gtgactttcg cgccgatgga gtcgccctct
    59101 tttttcagcg cgcgcatcag ttcatctaac gcgtcgattt tgtcggggtc cgggcagaag
    59161 aacggatttt gttcaaccag cgaccagtct ttgatttcca gcggaatgtc gcccatctgg
    59221 gtcaggcagc cgcggatttc aataccaaat ttctcggcga gatatttttt ggcaatcgcc
    59281 cctgccgcca cgcgcatggc ggtttcgcgg gcggaagaac gtccaccgcc gcgataatcg
    59341 cgcagaccgt atttttgttc gtaggtgtaa tcagcatggc ctggacggaa gacgtcttta
    59401 attgcgctgt aatcctgaga acgctggtcg gtattttcga tcaacaagcc aatgctggtg
    59461 ccagtagtaa cgccttcaaa aacaccggag agtattttga cctgatccgg ctcgcggcgc
    59521 tgggtggtat agcgcgatgt cccagggcga cgacgatcaa ggtcatgttg caggtccgct
    59581 tccgtcagcg gaatgcctgg cggaacacca tcgacgatgc agccgagcgc cagcccgtgc
    59641 gattcgccga aggtggttac gcgaaagagt tgtccaattg tgtttccagc catcacggct
    59701 ccgttattgt tgtgtttgcg tgtttactta atctttataa atcgcgaaat gttctcgtgc
    59761 ggcaagaagc tgctctttag tgagcataaa cacaccatcg ccgccgttat caaactccag
    59821 ccaggtgaac ggaacatcgg gatattgttc cataagatgt accatgctgt tgcccacttc
    59881 acaaatcaac acgccatcat cagcaaggta atctgccgcg ttaccgagaa tgcgacgcgt
    59941 cagtttcagg ccgtcggtgc cagatgccag gcccagttcc ggctcgtgac ggtattcgtt
    60001 tggcaggtcg gacatatctt ccgcgtcgac atacggcggg ttagtgacaa tcagatcgta
    60061 ttgcactttc ggcaaatcac ggaacagatc ggaacgaatc ggaatgacgt tgtggatcag
    60121 accgtgttct tcgatgttct gttcggcaac cgccagcgcg tcgggagaga tatctaccgc
    60181 gtcgacttcc gcttccggga aggcataagc acaggcaatg gcgatgcagc cgctaccggt
    60241 acacatatct aaaatatgct gcggttgctt gctgatgagt ccggcaaatt tattgttgat
    60301 cagttcacca atcggcgagc gcggcaccag cacgcgttca tcgacgtaaa attcatgacc
    60361 gcagaaccag gctttgttgg tcaggtaagc caccggaatg cgctcgttga cgcggcggat
    60421 cacgcgttca acaatacggt gtttttcgct ggaggtcaga cgcgcggtgc gcatatcttc
    60481 cgggatatcc agcggcaggt agagcgatgg taacaccagc tgtacggctt catcccacgg
    60541 gttatcggtg ccgtgaccgt accagatatt tgccgcgctg aagcggctta ccgaccagcg
    60601 caacatgtcc tgaatggttt gcagctcatt tactgcttca tcaacgaaaa ttttatccac
    60661 gtattcctcc agggcatgat cgcaataatt tcggcggcta gtttgccatg aagatgacga
    60721 taaatcagca ttcacgcgcg gtgagtgagg aaaaatacgt ttaaaacgat cgattgtgct
    60781 acgagtcggg taaactgtag gaaaattaga aatagagaca gataaatgaa aaagaaaaca
    60841 acacttagcg aggaggacca ggctctgttt cgccagttga tggcggggac tcgcaagatt
    60901 aagcaggaca cgattgtcca ccgaccgcag cgtaaaaaaa tcagcgaagt gccggtgaaa
    60961 cgcttgatcc aggagcaggc tgatgccagc cattatttct ccgatgagtt tcagccgtta
    61021 ttaaataccg aaggtccggt gaaatatgtt cgcccggatg tcagccattt tgaggcgaag
    61081 aaactgcgcc gcggcgatta ttcaccggaa ttgtttttgg atttacacgg tctgacgcag
    61141 ctgcaggcca agcaggaact gggcgcgttg attgccgcct gtcgccgcga acatgtgttt
    61201 tgtgcctgcg tgatgcatgg gcacgggaag catattttga agcaacaaac accgctgtgg
    61261 ctggcacaac atccgcatgt gatggcattt caccaggcac cgaaagagta cggcggtgat
    61321 gcggctttgt tggtgttgat tgaagtggaa gagtggttgc cgccggagtt gccctgaatg
    61381 ctggcagcaa atgccggagc agcgtaaacg ccttatccgg cctacaaata tgtgataatt
    61441 caataaattg caattcactt cgtaggcctg ataagacgca tcagagcaat actacgaact
    61501 tcagttcatt caatacatac tgaaaacgac acgtcgaatc aatcggttgc ccactactgg
    61561 tattcacatt caccacaata tcgtgtagta ctttatccgt taccggatga cgagttggcg
    61621 taaccgttac cgttgattta tcacaaccgt aagaggtaaa ggttaacaat tctgataccg
    61681 cgcggcagta tgctcctctg ccatcattat cagaaaaatt actgccatca cagagtctgc
    61741 cgccttccat caggtaggtc gaaagcgtgg tggtcactgt tgaccccgtg gactgaattc
    61801 gtaacgcctt attatggctg acataatttt cactccagga gatatcgtat gaagcgaccg
    61861 gtatcgttgg gcatattgtg ttatgcactg taggggccgt atagcttcca aatccgggta
    61921 aatctgtata ggaataaaag gatgcgatcc aagggcaacc ataatcatca gatgcaacgg
    61981 taattttaaa cgtcatatat gcacaacgaa aaattttcgt atctatgcca gggcaatttg
    62041 agttattaat tgtatcaaag tcagtcagta ccaaagttgg agctgaaccc gacccagacc
    62101 aggaaataac ccgcaacgta tcgcctttat acgcaaaaga atcatatttc ccatatgcaa
    62161 cctcctttgg cgttatcacc gtaacattga aaacactgct cgctacagca ccggttacct
    62221 tgtccatggc ataataaact ctcacactgt caaaactcgc actcataagt ttatctccag
    62281 cataactctt agatgcgccg acaacactca gaacgagcag cattaggctc gcgaaaatta
    62341 tggtccattt tttcatttca gaaatactca aaggttgccg tcactgtggc actaaagtct
    62401 ccggacgtta catctcgccc gctctttgcc tgtaaccagg tattaaaatt taggctgtta
    62461 tttccctgat tcagcgggag cgtgacgcct gttgtattgt tgattggcac cgatgtgcct
    62521 gccgcagttt caataccaat cccagcgccg gatgctgatg acgaggagtc aaaggccaga
    62581 aagccgggta gagcactatc ttctgttcct gtcaacgtta ctttgacatt aaacaatgta
    62641 gaactatgac aatctttcaa ttgaaacacc aatggcactc gctctgactg cccaaggttc
    62701 attaaatcgc tggcggcaat ggcaggaaaa tgtacttctg ccaactggct accattaatc
    62761 actggcgtac acgatttact gattaaattg ccggtaaagt gcaaattatc tcctgctatt
    62821 gtggttccac tgcccagcaa catcaggcta ctaataaaaa acaacgtacg ctttttcttc
    62881 attggtaatc tacctgtaac gtggcccagg cctcaaagtc cgcctctggc agaatgacac
    62941 cactcttttt tactggcaca gccgttaaca ccggtaaatc ggtttcattc accacaagtg
    63001 gcgtattgat cgtaaaactc tgccctgcct gtttgagctg aatgcctaaa ccagtgatat
    63061 tgctgcttac tgcagcaata tcaaaatcgc ttggtgtgcc actccagctc aaggtcattt
    63121 ccattaatgg gtcgcgaacc gccgtatcgc aggtaatgga atacggcacg gtttgctcat
    63181 aattgctgcc attaatgtca tcaataagca catcctgaaa agttatttca atggtttgag
    63241 cgttgctaat cgtgcagtta ggcggctgca ctaaataacc gtgaaaagag acgtttgaga
    63301 gtgccatcga gcaaaagccc cacagcaaaa taagcgagat ccgtttcatt ggtaatccac
    63361 cacaattgtg gcactggcgt taaattcagc tgccgctaac gttgtaccac tctgtttcac
    63421 cggcacagct tccagaacgg gaactcccga ggaaaaatta aatggcaacc atgagccaga
    63481 ggttaagtcg agtattgtgt gatcactact tttctgaacc cgaataccaa acccaggaat
    63541 acccgtgctg ataactgttt caccgttaat cacgaccgtt gtcgcctgca tctgtagacg
    63601 caggtcatcc attgccagat tgttacaaac aagcgagtag tcaatgggac gtttataatc
    63661 aaccctattg atcttattaa tgaatacatt accaaactca actgccgcac catttaccgt
    63721 acaaggagga ggggcatcga caagcacccg taaattcata cttactgact tactcgctgc
    63781 aactgcaaag ccgctaccaa cacaacatat cagtaaccca gttaccgccc agacagaaca
    63841 agatcctttc atactggctc tcccggcctc agcctttgcg gctctgctct tcatcgacct
    63901 agcaactgtt accgttgcaa gtgaaaaaga gcggcatccg agcgccatag tcgttgacat
    63961 aggtcagtac cggcacgcta cccatcttca cattcagcgg tgcataggtt ttcggcgaca
    64021 tcactattgg cgagaaaccg gctgccggat tgccattttt ctgtgtgctg gcattactga
    64081 aaatgacgta atacgacgtc gggttattga cggtaaattc ctgaccgttg cgggttaaag
    64141 taaccttata ttgctagggg tttttcatgc tgacgttctc cagcgccttc ggtcgccaga
    64201 acaacttaat acgcgtttgt agtgctattt gcagcgtgtt ggctttgttt gattttggcg
    64261 ggatctcacg cacgttgaag tagaacaggc tttcacgatc ggcaggcaat ttgttgatat
    64321 ccggcattcc ctgcactttt acctgcccgt tcatcattga atcgatgcgt tgcaccggtg
    64381 gcagaacagt taacggtgaa ctgatcttgt tacccttttc atcttcgatc cagctttggg
    64441 ccagataagg cagtttcgga tcgttattgc gcaacgtcac gctgattgat ttgtcgcttt
    64501 cattaaatat caaacgggta cgatctggcg ttacagaagc ctgggcagac aaactggcag
    64561 cgctaagcag taacgctagc gtcgtagcac ccaatttggc agagcaaagt aaatcagaca
    64621 tggatcattc cttaatcacg ttattggtta gctgaaacag aaacaggggg attggtgggt
    64681 gtcacctgtt gcgtctgctc ctgaatgaca ggctttatgt catcaggaac aaccggcgcg
    64741 acgttgcctt tatgctggca tggcagtaac aagccgttaa acagatcggc aggcagcggg
    64801 tccggcaggt tgatatcgca atgcgcaaca ccactccaga acacactcat gtgttcgcca
    64861 ggtttcaccc cagccagata aacattgccg tcatcatcga caaggcccac tgtctgctcg
    64921 ttatcatttt ttacttctgc gccaaacgga ggatggctgc cgtcttgtaa acgcagcacc
    64981 gccatcgctt tttgaccact gatgacggca aatttgcggt agccaatcgc cccttcagtt
    65041 agcgtggctt gtaccaccga ctgggttgct tcggcgtttt ccggcagttt gttgaggtcg
    65101 atatacgcct gattgcggta atagttattg acgtcagaaa cgacggcttt accaaacata
    65161 ttggtataaa cagcagcacc gttaccttct accggaacat cggcaacgcc atcggcatca
    65221 atcaacaagc gtgtcccgcc catattctgg gtacggtgaa gtgcgccacc gtgggcagta
    65281 agcgttgcgc cgccctgtaa cgacaagccc gcagaggtgt attgcccttc atggtagttc
    65341 gcactgaggt caacctgcgc cagcgaacca tcatggctgt aatagccgtc aacgctggtg
    65401 tgtttgtcac tggtgccgac gttcaactga tagtgagtcg cgtcatcgac acggctgaaa
    65461 taaccgacct gactgctgtc cgtcccactg ccatagtacc gttatagcta acggtactgt
    65521 tgtcgcccca cggcatactg agcgaaatgt acatgccttt gtcggcctgg ttgtcatact
    65581 cgtaacggta gccagtcatc gagatgctga cattacgaat actgcccata ttgaaatagt
    65641 gcgagagcat gatgttgtag tttgtctgct cctcgcgatc ccagtaggta tggcgggtat
    65701 agttgagata aaccgaaaca cccgcatcgc ggaagttctg gttataagtg gcggtgtaca
    65761 tctctttgtc gttgcccgtg cgtaccattc cgctgtcact ggcatccaga tactcgctca
    65821 tggtcataaa gttctcttcc gagaagcgat atccagcaaa agtaacgcgg ctgttgagct
    65881 ggtcaaagtc tttggaataa ctcacacgga aggagttacc gtccagcgaa cctttgccat
    65941 aagcggtgtc tttatccagt ttggtgtgcg agtgagtaac atcaaacgcg accgcgccga
    66001 atgtagacaa atcgcgaccg acaccaagcg ccgcagactg atagttttca tctcccagtg
    66061 cgccgccata taacgaccag ccgttagcga tcccccagga agcttcagca ccagaaaaaa
    66121 attcaccctc gacatggtgt ccccactctt gcggacggcc catcatgatc ttatagcgaa
    66181 cctgacctgg acgagtgagg tatggcatcg aggcggtgct gatgtcatat tcctgcacct
    66241 ggccgttctg ttcttcaatg cgaatatgca acgtaccgga gacagaatca ccaagatcct
    66301 gaatacgaaa cggtccagcc ggaacctgcg tttcgtaaat cacacgcccc atctggctga
    66361 cggtcacttt tgctgtggtg tgtgccacgc cggaaatgtc tggcgcgtag ccgcgcagat
    66421 tgggaggcaa catttgatcg tcagtactga cactgccacc aacatagtta aaaccatcaa
    66481 aaatatcgga tctgaggtaa tcctcgccca gcgccagttt ggcttttaat gatggtaacg
    66541 cccgcctggc atagtagcga ctccactccc attttttttg agtttcatcg ccgctgaatt
    66601 catcgtcatc attactgcga gtatgttgat agttagtctg ccagtcagca cgcatacgcc
    66661 acggccctag gttaacccca accgtcccgt tgccgctgat ctcgttacta tcatcaccgc
    66721 cattttcttc gtgccgggtt tgtgcgttga tgctgtagtc cgcgacgatc ccggagatgc
    66781 cgtcatccca acgtgaaggc ggatcccagt cgggataagt atattcgagg taagcctgtg
    66841 gcagtgaaat gactaatgcg gactggctta aatcagcctt aatttccatg ccttccagtt
    66901 gaccggattt caggcattta gcatcgtggc tccattgcag atttttcgcc acgtcttctt
    66961 ttaaaccaaa ctgcgctacc agttccggtg tcagacaagc atagcttttg ctcgcgtcat
    67021 cttcaccagc ataccagtaa atatcgtact cttccgccaa tggctgttta tttagttgaa
    67081 cctgtaaatt gtattttccg ggctcaacat atccctggct ggaaaaacgc ttcagatcaa
    67141 tttttgtgtc gccttttaat tccagaaaac gggaatcaaa ctgaatgtcg tcttcagccc
    67201 agacactgct ataactccct gacattgcca gcgcaatgca ccacggaaga atccgtaatc
    67261 gaaaaagaga atggtcaggc atacacgttt ctatccgttt ttattcttgt tcggtcaaaa
    67321 tccatttaaa tcccccatag cagtgtcgct atgggggtat gccttacagg taagtaatct
    67381 ggaaggtggt atttgcttca aaattaccca gatctggcgc atcagctgcg ccgaccagcc
    67441 aggctttaaa gttcagtgtc tgcttcgctt tgcctttgtt ggtagaagtg tctttcacta
    67501 ttttctggtc aatacccatg ccgcttttgt aagaggtacc cagagagtca ccgatcgcca
    67561 ggctaacatt gttaaatgcc gcaccggtat cggtattgaa aatggtgtaa taattgccgc
    67621 tatttgcaga agaaacggta ccagtaaagg tagtggtcat tgtttcctga gtatcaaata
    67681 cgcaatcttg cagacgaatc tggaaacttt tcggcgtggt ggtgccgtta tttttcagtt
    67741 tttcggcacc gatatcaccc atatcaactt ccagtttgtg atcgtccgga acgatagaac
    67801 acactgagtc ttcaatggtg ccgtaaaaac gtgcagtacc gttgttaccc gcagcgattg
    67861 ttgccgtaga ggtgaacacg cccatcacca ttgccgcagc aatagctgtt ttaacaaact
    67921 tactcattat ttgtccttaa actataaata attaaaaata taaacattgc aatacattga
    67981 ttcagtcaat agccaatgtt tatacccagc cattgaatac ggcatgcgac ccaaaaagaa
    68041 caggtcgtag aaaattgtat tcattggtgt ggggccgcat agtgatgttt ccacacccct
    68101 gatccaattt aattttacta aagagtaaat gtagatttaa ttaatatatt gacagaagaa
    68161 aggttatctt aaggacatcc ttaaaaaata cggaataagt atttagaaaa aatagaaagt
    68221 atatcaataa tgaatatcaa cagcttaaaa aacaaacaat gaacatataa tgcgattggc
    68281 atttatccac ttttgacaat ataatatata aacactatag ccagaattta atcaagtttc
    68341 aattacatcc tgcctgatta ttcaactcta ttttctctat tatctgtatt aataaatgac
    68401 actgagtgtc aaacagacat tattcatttc aacatgtaac tttgggcaaa atcaagatac
    68461 gcgcagaaga ataatttccg aatgaatgta gaaagtgttt tatttataga taaggagagt
    68521 cttcgatagt agtgaggttg acagatatcc gctgcctaat gagcaattat gcccaatgaa
    68581 tctacctcat tgggcataat ttggaacaca tcagatagct tttgccatct tcagattaca
    68641 cgggctcatc tgccagttga atgtaccgtt tccgctttca tcaagcgtta cgctggcaat
    68701 ggccgaggtg gtaaacatag gcggcgtttc gcccggacat aactcggcaa ccagataccc
    68761 gactaacggt aagtgagaga tcaccagtac cgaagcaaca ccttcattgg tcaacgcctg
    68821 caaataggca ctgaccagac caacatcgcc gcagggcgtt aactccggca gaacttctgc
    68881 actggaaggc aggttcagac aatcccctac ttcttccagt gtttgctcgg ctcgcaggaa
    68941 cgggctcacc agaacgcgtt cgatttccac tttttgacct ttcagccagt tcgccatcag
    69001 gcgagattcg tcacaaccat tagtggtcag aggacgaacc gaatcactgg cggcatcgag
    69061 ggctgcgtcg ccgtgacgca tgataaaaac ttgcatattg caccgctttt gttaaccaga
    69121 ttcgcccgtt ttctttcatc tgaaaccaga gaaagaaaac ggtggccggc attgtgcctt
    69181 atccattcac cgaatgaaac gctgtttttt acctcaatgg cgtaagtata gtcaatatgc
    69241 gtttacattt tgaccaacac tccgccattc agcgcggatt catatagctt tgaccttctt
    69301 attgcaggtc agttgcagtt gttttccaaa aactttcccc acgcgcgccc atctcgacca
    69361 aacgctcgca aggggtaaaa cgggaaccat actgcgtggc cagtcgttgc attattgcaa
    69421 ccacttcgcc cgcgccgaga gaatcgatat agcggaacgg tccaccgaga aatggcggaa
    69481 aaccaatgcc aaataccgca ccaatatccc cgtcacgcac gctacggata acctgctcat
    69541 caacacaacg tactgcttca ttcagcatca acatcacaca ccgttcagca acctgcggtg
    69601 cggagagtcg cccctgccct tgtgcgccaa tgagcgggta aatggcagga tcgacctgtt
    69661 ttttgctttt acgccctttc tgaccataaa gatagaaacc ccgaccattt tttctgcctt
    69721 tgcgatcgtc gttcaaaatt gaagaaacaa catttgcagg cgcgctaaaa cgttctccat
    69781 aagcggcttc cagtacagga ataattttag tcccggtgtc gattcctacc tcatccaaaa
    69841 gttggattgg gcctaccgga aaaccaaatt tcactagcgc ggcatcaatg tgctctaccc
    69901 gttcaccttc ggtcagcatg cggatagctt cattaatgta aggcgctaag atgcgattga
    69961 cgtaaaaccc ggctttgtca cgcacgacaa tgggcgtttt gccctgtttt ttcgccagtt
    70021 ttaccgtagt ggcgatggtt tgcgccgatg tacccgcatg aggaataatc tctaccagcg
    70081 gcattttttc caccggactg aagaaatgca ggccaataac ttgctcaggt cgcgcggcgt
    70141 gagcggcgat atcaccaatc ggtaaagatg acgtgttcga agcaaaaatg gtatgagcgg
    70201 cgcaattttg ctccacttcc gccaccatct gttgttttaa ttcgagattt tcaaacaccg
    70261 cttcaataat cagatcgcga tgggcaaagc cgcgatagtc cgtcgttccg gagattaatg
    70321 ccagctgttt gtcacgttcg ctggctttga gatgacgacg gcgaactttg ccctccagct
    70381 gatcccaact gtacttcagc gcatgattta tgccctgcgg gttgatatct ttaattctga
    70441 ccggaagccc cgctttacaa gcagtgacat aagcaatacc gccgcccatc aagccaccac
    70501 ctaaaattcc cacgctgttt aatggcgcag gcggtgcatc actaccggga tctttcttca
    70561 cgtccgtact ggcaaaaaag atactacgca aagcttgcga ttgcggcgtc atcgccagtt
    70621 cgccaaaggc gcgagcttca gcgtcatagc cgctgctggt gccctgcgct aatcccgttt
    70681 caacaacctc caggatgcgt tctgtcgccg gataattgcc ttgagttttg tgttctgttt
    70741 tcttgccgac cattttgaac agcagcgcac gacctaacgg ccccgccaga atacgctcgc
    70801 gtacaggtag agggcgggaa gatgggcgat ccttcttcgc cagctcaaca gcggcttcca
    70861 gcaaaatgga gtgcggaaca acgtcatcca ccagccccag ctttaatgcc tgtttcgccc
    70921 gaagttgttt tccggtgaga atcatctcta atgctgtact gacgcctatc agacgcggta
    70981 aacgctgggt gccgcctgaa ccgggtaaca atccaagttg tacttcaggc aaaccgagca
    71041 ccgttttagg atcgtcagta caaacgcgac cgtggcacgc cagcgccagc tccagcccac
    71101 cgcccaggca agcgccatga atagccgcga taaccggaat gggcaaagca tgaatctccg
    71161 ccatcaactg ttggccctgc cgcgccagtg cttccgcttc ttgcgccgtt ttgcagttgc
    71221 caatcatgtt gatgtctgct ccggcgataa aattgtccgg tttcgcggag ataaacacca
    71281 cgccgcgcaa ctctttgttt tcacggagtt gcttaataat ggcgcgcacc tgcgaggcaa
    71341 attccgcctt cagggtattc attttctcat ccggtacgtc gatggtgatg atagcaatgt
    71401 tgtccagacg aacattaaga gtaaacgctg atgccatttc cattattccg cctccagaac
    71461 cattgccgcg ccaagcccac cggcagcaca ggcggtaact aaaccaaatc caccgccgcg
    71521 acggcgaagt tcatgcaatg tctgggtaat catccgcgcg ccggtcgccg cgaagggatg
    71581 tccgtaagca atcgaaccgc caagcacgtt aaatttgcta tcgtccactt cgccagtggc
    71641 atgtgcacgc cccagtactt cacgagcaaa acgttcacta cccagcaact gaatattcgc
    71701 cagtgtctga gctgcaaagg cttcgtgcat atcgatcaat gtcagttcgc tcatcgtcaa
    71761 accggcacgt tccagcgcca gcggtgttga ccaggctgga ccgagcaaca tgtcctgcca
    71821 gacatcaatc gcagtaaatg cgtagctgcg cagatacccc agcggcacca gccctaattc
    71881 tttcgcccgg gattcagtca tcaggatcac cgctgccgcg ccatcggtca gcggcgtact
    71941 gtttgccgcc gttaccgttc cgtgtttgcg atcaaacgcc gggcgcagct ttgcgtaatc
    72001 ggcaagcgag gaattaccgc gaatattgtt gtcttcgaca agcggttgtt tataaggagg
    72061 gataaaggca gtcatcacct cttctttgag ttttccgtct gaccatgcct gagcggcacg
    72121 ctgatgcgaa cggtgcgcta atgcatcttg ctgttctcgg gtgatgccgt aggttttcgc
    72181 catttgttcg gcggtgtcgc ccatccgcaa gccagtagaa tattctgcta ccgcaggcgg
    72241 tacgggcatt aagtcgcgca aacgcaggcg agagaagagt ttcaggcgct ggctcatggt
    72301 tcgagctttg ttgacatcaa ccagcacgtg cgccagtttt ttactgacgc caattggcaa
    72361 tacggaagag gaatctgccc caccggcaat ccccgctcgg atagttcccg ccatcaggct
    72421 ttctgcgacg tttgcaaccg cctggaaact ggtagcgcaa gcgcggctga cgctgtaagc
    72481 atcggtatgc acattcattc cggtaccgag aacaatttca cgcgcaatgt tgggggcttc
    72541 aggcatttgt acgacctgac caaagaccag ctgttcaatc acttcagcgg ggatctcgct
    72601 gcgtgccagc agttcgccta ccaccatctt ccctaaatca accgcgggaa tgccatgaaa
    72661 agccgtcgcc tggcgggcaa aaggcgtacg taaaccgcta acaatggcga tacgatcgcc
    72721 ctggcgggta accagtggta aaacctgacc cataacactc ccctgtaaaa aaataaataa
    72781 agtggtctga cctgatcata gtattaacca tttttttaca tttagccaag cggagaaaaa
    72841 ggaaagtggg agctatgaca cagtgaagac agaagaaaga aaagaaaacg cccctgccag
    72901 caactgacag gggcgtacgt acccgagagg aattaacgca gacccagctg gaagatcagc
    72961 atttcagctt cgcaggcaaa agtgaaatcg atatccaggc gcacaccgtc agactcttca
    73021 gtgaaagtcg gggagatttt gcacggttcg gattccacgc tacgggattt ttcagtcagc
    73081 gccgccagcg tttgttctgc ttctgcgcgg tttgcaaaca cgcggctgta agacgcggtg
    73141 cagtcggagt tgtccataat ggtgccaaca tccatacagc agcaaaccgg ggtttcatca
    73201 gcactacatt tactcatcgt tgatttcctc tgtatgtgca cccaaggtgc cagataaacg
    73261 ttgtggatat tttacgcttc cggaaagtgc tgctccagtt gttaattctg caaaatcgga
    73321 taagtgaccg aaatcacact taaaaatgat ctaaaacaaa attcacccga atccgcgagt
    73381 gcgcaacctc caaattttgc cagctggatc gcgtttctta gatcatattt gaaaaaagat
    73441 ggaaacatac ttgcaacatt ctagctggtc cgacctatac tctcgccact ggtctgattt
    73501 ctaagatgta cctcagaccc tacacttcgc gctcctgtta cagcacgtaa catagtttgt
    73561 ataaaaataa atcattgagg ttatggtcat gagccagaaa accctgttta caaagtctgc
    73621 tctcgcagtc gcagtggcac ttatctccac ccaggcctgg tcggcaggct ttcagttaaa
    73681 cgaattttct tcctctggcc tgggccgggc ttattcaggg gaaggcgcaa ttgccgatga
    73741 tgcaggtaac gttagccgta accccgcatt gattaccatg tttgaccgcc cgacattttc
    73801 tgcgggtgcg gtttatattg acccggatgt gaatatcagc ggaacgtctc cgtctggtcg
    73861 tagcctgcaa gccgataaca tcgcgcctac ggcatgggtt ccgaacatgc actttgttgc
    73921 accgattaac gaccaatttg gttggggcgc ttctattacc tctaactatg gtctggcaac
    73981 agagtttaat gatacttatg caggcggctc tgtcgggggt acaactgacc ttgaaaccat
    74041 gaacctgaac ttaagcggtg cgtatcgctt aaataatgca tggagctttg gtcttggttt
    74101 caacgccatc tacgctcgcg cgaaaattga acgtttcgca ggcgatctgg ggcagttggt
    74161 tgctggtcag attatgcaat ctcctgccgg gcaaactcct caagggcaag cattggcagc
    74221 taccgccaac ggtatcgaca gtaataccaa aatcgctcat ctgaacggta accagtgggg
    74281 ctttggctgg aacgccggaa tcctgtatga actggataaa aataaccgct atgcactgac
    74341 ctaccgttct gaagtgaaaa ttgacttcaa aggtaactac agcagcgatc ttaatcgtgc
    74401 gtttaataac tacggtttgc caattcctac cgcgacaggt ggcgcaacgc aatcgggtta
    74461 tctgacgctg aacctgcctg aaatgtggga agtgtcaggt tataaccgtg ttgatccaca
    74521 gtgggcgatt cactatagcc tggcttacac cagctggagt cagttccagc agctgaaagc
    74581 gacctcaacc agtggcgaca cgctgttcca gaaacatgaa ggctttaaag atgcttaccg
    74641 catcgcgttg ggtaccactt attactacga tgataactgg accttccgta ccggtatcgc
    74701 ctttgatgac agcccagttc cggctcagaa tcgttctatc tccattccgg accaggaccg
    74761 tttctggctg agtgcaggta cgacttacgc atttaataaa gatgcttcag tcgacgttgg
    74821 tgtttcttat atgcacggtc agagcgtgaa aattaacgaa ggcccatacc agttcgagtc
    74881 tgaaggtaaa gcctggctgt tcggtactaa ctttaactac gcgttctgat aacgcgttcg
    74941 cctggataaa gtcacctgct atgcaggtga ctttaactcc cccacttcac cgaaagtagt
    75001 ttcctcattg cttgcctcct gagttttgca aacagcctga tggcagcttg ccctttcaaa
    75061 tcagtaagtg gtatccacag acaaacctga agggaaatgc atattttcaa gcgttctgct
    75121 cgtccttctc aaagagttta cttttctgca tttccaggat actcaacccc cctggctatt
    75181 gtgcgcttat acactcaaat taaagatagg ttctaaataa atgagcgttt tttgatagtc
    75241 tatttcatta ggtaatatat atttgtaaca aatcaatcaa catagaataa aatcatgcta
    75301 ccatctattt caatcaacaa taccagcgca gcttacccag aatccatcaa tgaaaataac
    75361 aatgatgaaa ttaatggatt agtacaagag ttcaaaaacc tttttaatgg taaggaagga
    75421 ataagcacct gtattaaaca tctacttgag cttataaaaa acgccatacg agtaaacgac
    75481 gatccttata gatttaatat taataattcc tcagttactt atattgatat tggctccaat
    75541 gatacagacc atattactat tggtatcgac aaccaataga attacctgcg aactataaag
    75601 acaaagaact cgtccgtact atcattaatg acaacatagt tgagaagact catgatatca
    75661 ataacaagga aatgatcttc agcgcattaa aagaaatata tgatggagat cctggtttta
    75721 tcttcgataa gatatcacac aaactcagac atacggtaac ggaatttgat gagagcggga
    75781 aaagcgaacc aacggactta tttacctggt acggtaaaga taaaaaaggc gactctctcg
    75841 ctattgtaat taaaaataaa aacggaaatg attacttatc tctcggttac tacgatcagg
    75901 acgactacca cattcaaaga ggaattcgta ttaatggtga tagtctcacc caatattgta
    75961 gtgaaaacgc caggagtgct tcagcgtggt ttgaaagcag taaagctatc atggcagaat
    76021 catttgcaac tggttccgat catcaggttg taaacgagct caacggggaa agactgagag
    76081 aacctaacga agtttttaaa cgtttaggtc gagcaataag atataatttt caagtggacg
    76141 atgcaaaatt tagacgcgat aatgtaaaag aaataatttc tactttattc gctaacaaag
    76201 ttgacgttga ccatcctgaa aataaataca aagactttaa ggatctcgaa gataaagttg
    76261 aaaaaagact tcaaaattgc caggcgaaat atcaaaatga aattaatcaa ctatctgcac
    76321 taggtgttaa ttttgatgat atttaaaatt atttttccag ttttttgatt tttcaacgcc
    76381 tgaatatcgg aaatagtcag tgacaaa
//
LOCUS       DADMEQ010000005        68994 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.005, whole genome shotgun sequence.
ACCESSION   DADMEQ010000005 DADMEQ010000000
VERSION     DADMEQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 68994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68994
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.005"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..188
                     /locus_tag="JNZ05_000339"
     CDS             <1..188
                     /locus_tag="JNZ05_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8563697.1"
                     /translation="GIRIENLGGGVANIPFQNGILPVDPSGHGTVNMRAWPVNLVGGE
                     LETGKFQGTATITVMVR"
     gene            complement(231..1091)
                     /gene="rsmI"
                     /locus_tag="JNZ05_000340"
     CDS             complement(231..1091)
                     /gene="rsmI"
                     /locus_tag="JNZ05_000340"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAY8563698.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1156..3192
                     /locus_tag="JNZ05_000341"
     CDS             1156..3192
                     /locus_tag="JNZ05_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAY8563699.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNISTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLSVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3150..3545
                     /locus_tag="JNZ05_000342"
     CDS             3150..3545
                     /locus_tag="JNZ05_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAY8563700.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            3565..4155
                     /gene="diaA"
                     /locus_tag="JNZ05_000343"
     CDS             3565..4155
                     /gene="diaA"
                     /locus_tag="JNZ05_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HAY8563701.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4165..4740
                     /gene="yraP"
                     /locus_tag="JNZ05_000344"
     CDS             4165..4740
                     /gene="yraP"
                     /locus_tag="JNZ05_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HAY8563702.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(4854..5894)
                     /locus_tag="JNZ05_000345"
     CDS             complement(4854..5894)
                     /locus_tag="JNZ05_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY8563703.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNGLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(5967..6602)
                     /locus_tag="JNZ05_000346"
     CDS             complement(5967..6602)
                     /locus_tag="JNZ05_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAY8563704.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6730..7248
                     /gene="yhbO"
                     /locus_tag="JNZ05_000347"
     CDS             6730..7248
                     /gene="yhbO"
                     /locus_tag="JNZ05_000347"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HAY8563705.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTSAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7228..7671)
                     /locus_tag="JNZ05_000348"
     CDS             complement(7228..7671)
                     /locus_tag="JNZ05_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HAY8563706.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            7722..8024
                     /gene="yhbQ"
                     /locus_tag="JNZ05_000349"
     CDS             7722..8024
                     /gene="yhbQ"
                     /locus_tag="JNZ05_000349"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HAY8563707.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8011..8514)
                     /locus_tag="JNZ05_000350"
     CDS             complement(8011..8514)
                     /locus_tag="JNZ05_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY8563708.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8508..9020)
                     /locus_tag="JNZ05_000351"
     CDS             complement(8508..9020)
                     /locus_tag="JNZ05_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HAY8563709.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDVLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAARKQD
                     PDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMKTAPE
                     TKQTSVGEPC"
     gene            9229..10224
                     /locus_tag="JNZ05_000352"
     CDS             9229..10224
                     /locus_tag="JNZ05_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY8563710.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHISINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10233..11111
                     /locus_tag="JNZ05_000353"
     CDS             10233..11111
                     /locus_tag="JNZ05_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY8563711.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            11192..12199
                     /locus_tag="JNZ05_000354"
     CDS             11192..12199
                     /locus_tag="JNZ05_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAY8563712.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSIQQAFVKLRRGEIGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12317..13561)
                     /gene="mtr"
                     /locus_tag="JNZ05_000355"
     CDS             complement(12317..13561)
                     /gene="mtr"
                     /locus_tag="JNZ05_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAY8563713.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13715..15604)
                     /locus_tag="JNZ05_000356"
     CDS             complement(13715..15604)
                     /locus_tag="JNZ05_000356"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH family ATP-dependent RNA helicase"
                     /protein_id="HAY8563714.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGELRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15597..15677)
                     /gene="yrbN"
                     /locus_tag="JNZ05_000357"
     CDS             complement(15597..15677)
                     /gene="yrbN"
                     /locus_tag="JNZ05_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HAY8563715.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(15784..16668)
                     /gene="nlpI"
                     /locus_tag="JNZ05_000358"
     CDS             complement(15784..16668)
                     /gene="nlpI"
                     /locus_tag="JNZ05_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HAY8563716.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(16777..18912)
                     /gene="pnp"
                     /locus_tag="JNZ05_000359"
     CDS             complement(16777..18912)
                     /gene="pnp"
                     /locus_tag="JNZ05_000359"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAY8563717.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19159..19428)
                     /gene="rpsO"
                     /locus_tag="JNZ05_000360"
     CDS             complement(19159..19428)
                     /gene="rpsO"
                     /locus_tag="JNZ05_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAY8563718.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(19577..20521)
                     /gene="truB"
                     /locus_tag="JNZ05_000361"
     CDS             complement(19577..20521)
                     /gene="truB"
                     /locus_tag="JNZ05_000361"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAY8563719.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20521..20922)
                     /gene="rbfA"
                     /locus_tag="JNZ05_000362"
     CDS             complement(20521..20922)
                     /gene="rbfA"
                     /locus_tag="JNZ05_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAY8563720.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21086..23734)
                     /gene="infB"
                     /locus_tag="JNZ05_000363"
     CDS             complement(21086..23734)
                     /gene="infB"
                     /locus_tag="JNZ05_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAY8563721.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(23759..25246)
                     /gene="nusA"
                     /locus_tag="JNZ05_000364"
     CDS             complement(23759..25246)
                     /gene="nusA"
                     /locus_tag="JNZ05_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAY8563722.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(25274..25726)
                     /gene="rimP"
                     /locus_tag="JNZ05_000365"
     CDS             complement(25274..25726)
                     /gene="rimP"
                     /locus_tag="JNZ05_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAY8563723.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(25933..26009)
                     /locus_tag="JNZ05_000366"
     tRNA            complement(25933..26009)
                     /locus_tag="JNZ05_000366"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25973..25975),aa:Met,seq:cat)
     gene            26357..27700
                     /gene="argG"
                     /locus_tag="JNZ05_000367"
     CDS             26357..27700
                     /gene="argG"
                     /locus_tag="JNZ05_000367"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAY8563724.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(27708..29315)
                     /locus_tag="JNZ05_000368"
                     /pseudo
     CDS             complement(27708..29315)
                     /locus_tag="JNZ05_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(29793..29879)
                     /locus_tag="JNZ05_000369"
     tRNA            complement(29793..29879)
                     /locus_tag="JNZ05_000369"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29843..29845),aa:Leu,seq:gag)
     gene            complement(29894..30226)
                     /gene="secG"
                     /locus_tag="JNZ05_000370"
     CDS             complement(29894..30226)
                     /gene="secG"
                     /locus_tag="JNZ05_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAY8563725.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(30454..31791)
                     /gene="glmM"
                     /locus_tag="JNZ05_000371"
     CDS             complement(30454..31791)
                     /gene="glmM"
                     /locus_tag="JNZ05_000371"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAY8563726.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(31784..32632)
                     /gene="folP"
                     /locus_tag="JNZ05_000372"
     CDS             complement(31784..32632)
                     /gene="folP"
                     /locus_tag="JNZ05_000372"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAY8563727.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(32722..34656)
                     /gene="ftsH"
                     /locus_tag="JNZ05_000373"
     CDS             complement(32722..34656)
                     /gene="ftsH"
                     /locus_tag="JNZ05_000373"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAY8563728.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(34756..35385)
                     /gene="rlmE"
                     /locus_tag="JNZ05_000374"
     CDS             complement(34756..35385)
                     /gene="rlmE"
                     /locus_tag="JNZ05_000374"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HAY8563729.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            35511..35804
                     /gene="yhbY"
                     /locus_tag="JNZ05_000375"
     CDS             35511..35804
                     /gene="yhbY"
                     /locus_tag="JNZ05_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAY8563730.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(35960..36436)
                     /gene="greA"
                     /locus_tag="JNZ05_000376"
     CDS             complement(35960..36436)
                     /gene="greA"
                     /locus_tag="JNZ05_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAY8563731.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36684..38117
                     /gene="dacB"
                     /locus_tag="JNZ05_000377"
     CDS             36684..38117
                     /gene="dacB"
                     /locus_tag="JNZ05_000377"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY8563732.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGILKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFGVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(38303..39475)
                     /gene="cgtA"
                     /locus_tag="JNZ05_000378"
     CDS             complement(38303..39475)
                     /gene="cgtA"
                     /locus_tag="JNZ05_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HAY8563733.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(39491..40456)
                     /locus_tag="JNZ05_000379"
     CDS             complement(39491..40456)
                     /locus_tag="JNZ05_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY8563734.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLSPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(40583..40840)
                     /gene="rpmA"
                     /locus_tag="JNZ05_000380"
     CDS             complement(40583..40840)
                     /gene="rpmA"
                     /locus_tag="JNZ05_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAY8563735.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(40861..41172)
                     /gene="rplU"
                     /locus_tag="JNZ05_000381"
     CDS             complement(40861..41172)
                     /gene="rplU"
                     /locus_tag="JNZ05_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAY8563736.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            41431..42402
                     /gene="ispB"
                     /locus_tag="JNZ05_000382"
     CDS             41431..42402
                     /gene="ispB"
                     /locus_tag="JNZ05_000382"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HAY8563737.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            42630..42908
                     /gene="sfsB"
                     /locus_tag="JNZ05_000383"
     CDS             42630..42908
                     /gene="sfsB"
                     /locus_tag="JNZ05_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HAY8563738.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPRTHEFIDRTQLMRSYTKPKK"
     gene            complement(42956..44215)
                     /gene="murA"
                     /locus_tag="JNZ05_000384"
     CDS             complement(42956..44215)
                     /gene="murA"
                     /locus_tag="JNZ05_000384"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAY8563739.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(44270..44524)
                     /gene="ibaG"
                     /locus_tag="JNZ05_000385"
     CDS             complement(44270..44524)
                     /gene="ibaG"
                     /locus_tag="JNZ05_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HAY8563740.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44684..44977)
                     /gene="mlaB"
                     /locus_tag="JNZ05_000386"
     CDS             complement(44684..44977)
                     /gene="mlaB"
                     /locus_tag="JNZ05_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HAY8563741.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(44977..45612)
                     /gene="mlaC"
                     /locus_tag="JNZ05_000387"
     CDS             complement(44977..45612)
                     /gene="mlaC"
                     /locus_tag="JNZ05_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HAY8563742.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(45631..46182)
                     /gene="mlaD"
                     /locus_tag="JNZ05_000388"
     CDS             complement(45631..46182)
                     /gene="mlaD"
                     /locus_tag="JNZ05_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HAY8563743.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46187..46969)
                     /gene="mlaE"
                     /locus_tag="JNZ05_000389"
     CDS             complement(46187..46969)
                     /gene="mlaE"
                     /locus_tag="JNZ05_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HAY8563744.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHVPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(46977..47786)
                     /gene="mlaF"
                     /locus_tag="JNZ05_000390"
     CDS             complement(46977..47786)
                     /gene="mlaF"
                     /locus_tag="JNZ05_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HAY8563745.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            47996..48961
                     /locus_tag="JNZ05_000391"
     CDS             47996..48961
                     /locus_tag="JNZ05_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HAY8563746.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLLLGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIIWLAMLYWLSPILVE"
     gene            48975..49961
                     /gene="kdsD"
                     /locus_tag="JNZ05_000392"
     CDS             48975..49961
                     /gene="kdsD"
                     /locus_tag="JNZ05_000392"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HAY8563747.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            49982..50548
                     /gene="kdsC"
                     /locus_tag="JNZ05_000393"
     CDS             49982..50548
                     /gene="kdsC"
                     /locus_tag="JNZ05_000393"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HAY8563748.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDCPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            50545..51120
                     /gene="lptC"
                     /locus_tag="JNZ05_000394"
     CDS             50545..51120
                     /gene="lptC"
                     /locus_tag="JNZ05_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HAY8563749.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            51089..51646
                     /gene="lptA"
                     /locus_tag="JNZ05_000395"
     CDS             51089..51646
                     /gene="lptA"
                     /locus_tag="JNZ05_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HAY8563750.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51653..52378
                     /gene="lptB"
                     /locus_tag="JNZ05_000396"
     CDS             51653..52378
                     /gene="lptB"
                     /locus_tag="JNZ05_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HAY8563751.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            52426..53859
                     /gene="rpoN"
                     /locus_tag="JNZ05_000397"
     CDS             52426..53859
                     /gene="rpoN"
                     /locus_tag="JNZ05_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAY8563752.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            53882..54169
                     /gene="hpf"
                     /locus_tag="JNZ05_000398"
     CDS             53882..54169
                     /gene="hpf"
                     /locus_tag="JNZ05_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HAY8563753.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54287..54778
                     /gene="ptsN"
                     /locus_tag="JNZ05_000399"
     CDS             54287..54778
                     /gene="ptsN"
                     /locus_tag="JNZ05_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HAY8563754.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            54824..55678
                     /gene="rapZ"
                     /locus_tag="JNZ05_000400"
     CDS             54824..55678
                     /gene="rapZ"
                     /locus_tag="JNZ05_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAY8563755.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55675..55947
                     /gene="npr"
                     /locus_tag="JNZ05_000401"
     CDS             55675..55947
                     /gene="npr"
                     /locus_tag="JNZ05_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HAY8563756.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            56160..56792
                     /gene="yrbL"
                     /locus_tag="JNZ05_000402"
     CDS             56160..56792
                     /gene="yrbL"
                     /locus_tag="JNZ05_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HAY8563757.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(56789..57517)
                     /gene="mtgA"
                     /locus_tag="JNZ05_000403"
     CDS             complement(56789..57517)
                     /gene="mtgA"
                     /locus_tag="JNZ05_000403"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HAY8563758.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSTPDGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(57514..58167)
                     /gene="elbB"
                     /locus_tag="JNZ05_000404"
     CDS             complement(57514..58167)
                     /gene="elbB"
                     /locus_tag="JNZ05_000404"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HAY8563759.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58397..60733)
                     /gene="arcB"
                     /locus_tag="JNZ05_000405"
     CDS             complement(58397..60733)
                     /gene="arcB"
                     /locus_tag="JNZ05_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HAY8563760.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSHRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(60829..61758)
                     /locus_tag="JNZ05_000406"
     CDS             complement(60829..61758)
                     /locus_tag="JNZ05_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAY8563761.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            62433..66893
                     /gene="gltB"
                     /locus_tag="JNZ05_000407"
     CDS             62433..66893
                     /gene="gltB"
                     /locus_tag="JNZ05_000407"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HAY8563762.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIHPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            66906..68324
                     /locus_tag="JNZ05_000408"
     CDS             66906..68324
                     /locus_tag="JNZ05_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY8563763.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            68508..68987
                     /locus_tag="JNZ05_000409"
     CDS             68508..68987
                     /locus_tag="JNZ05_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HAY8563764.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        16843 a        18747 c        17152 g        16252 t
ORIGIN      
        1 tcggtattcg tattgaaaat ctcggcggcg gagttgcaaa tattcctttt cagaacggta
       61 tccttcccgt tgatccttcc gggcatggca ccgtcaatat gcgcgcctgg ccagttaatc
      121 tggtcggtgg tgagctggaa acaggaaaat ttcagggcac agccaccatt accgtcatgg
      181 tgcggtaaaa gaagacgttg cgttttgatt aacgcaacgc ttcggcactg ttacccctgc
      241 tgctccagcg catacttata cagcgcattt ttcttcacgc catgaatttc tgcggccagc
      301 gccgccgctt ttttcagcgg cagttctgcc tgtagcagcg ccagcgtgcg cagggcatcg
      361 gcgggtaagt cttcttcctg tgctttatga ccttcgacaa tcagcaccat ttcgcctttg
      421 cgacggtttt catcttcctt tacccacgcc agcagctcgc caacgggcgc gccgtgaatg
      481 gtttcccagg ttttggtcag ctcacgcgcc agaaccacgt agcgggattc gcctaatacc
      541 gcaacgatat cttccaggct atctaacaga cggtgggtag attcataaaa aatcagcgtg
      601 cgcggctccg cttcaatggc ttttagcgca tcacggcggc cttttgattt ggcaggtaaa
      661 aagccttcgt aacagaaacg gtcagatggt aaacccgcgg cgcttaacgc agtgatagca
      721 gcacacggtc cgggtagcgg caccacacga atccccgctt cacggcaggt acgcaccaga
      781 tggtagccag gatcgttaat tagcggcgtt ccggcatcgg aaaccagcgc aatgttttgc
      841 ccctcttgca gcttcgccag cagcgtttcg gctttttgtt gttcgttatg gtcgtgcagc
      901 gcaaacaacc gggcattaat cccaaaatgt tgcagcaata aacctgtgtg acgagtatcc
      961 tcggcggcaa tcagatcaac ggcctgtaat acctctaacg tacgctgggt gatatccgcc
     1021 agattgccga ttggcgtcgg tacaatgtaa agctggccct gagaattatc cgccgattgg
     1081 tgttgtttca ttgtgtcgtc cgtattgccg atttaatatt gagcattgcg taaaaaaaat
     1141 atcactggat acattatggt accctcaaca ttttctcgtt tgaaagccgc gcgttgtctg
     1201 cctgttgttc tggcagccct gattttcgcc ggttgtggca cccatactcc cgatcagtcc
     1261 actgcttata tgcagggcac ggcgcaggct gattctgcct tttatcttca gcagatgcag
     1321 caaagctctg atgataccag gatcaactgg caattactcg ccattcgtgc actggtgaaa
     1381 gaaggtaaaa ccgggcaggc ggttgagttg tttaaccaac taccgcaaga actgaacgat
     1441 tctcagcgtc gcgagaaaac actgctggcg gcagagatta aactggcgca gaaagatttt
     1501 gctggcgcgc aaaacttgct ggcgaaaatc acacctgccg atttagaaca aaaccagcaa
     1561 gcgcgttact ggcaggcaaa aatcgatgcc agccaggggc gtccttccat tgatttactg
     1621 cgcgcgttaa ttgctcagga accgctgctt ggcgcgaaag aaaaacagca gaatattgat
     1681 gccacctggc aggcgctctc ctccatgact caggaacagg cgaatacgct ggtgatcaac
     1741 gccgacgaaa atattctgca aggctggctg gatctgcagc gcgtctggtt tgataaccgt
     1801 aacgatcccg acatgatgaa agccgggatc gccgactggc agaaacgtta tccgaacaat
     1861 ccgggcgcga aaatgctgcc aacgcagttg gttaacgtaa aagcgtttaa accagcctcg
     1921 accaacaaaa tcgccctgct gttgccgctg aatggccagg cagcggtatt tggtcgcact
     1981 attcagcaag gctttgaagc cgcgaaaaat atcagcactc agccagtggc ggctcaggta
     2041 gctgccgcac ctgccgcaga cgtagcagaa caacctcagc cgcaaactgc ggatggcgtt
     2101 gccagcccgg cacaagcctc ggttagcgat ctgaccggtg atcagcctgc ggcccagccg
     2161 gtgcctgtaa gcgccccggc gacaagcacc gcagcggtaa gcgcacccgc aaatccatcc
     2221 gcagagctga aaatctacga tacctcatca caaccactta gccagatctt aagccaggtt
     2281 cagcaggatg gcgcgagtat tgtggttggt ccgttgctga aaaataacgt tgaagagttg
     2341 ctgaagagca acaccccgct gagcgtactg gcactgaacc agccggagaa tatcgaaaac
     2401 cgcgtcaata tttgttactt cgcgctttca ccggaagacg aagcgcgcga tgcagcgcgt
     2461 catattcgtg accagggtaa acaagcgccg ctggtgctga tcccacgcag tgcattgggc
     2521 gatcgcgtag ccaatgcgtt tgcgcaagag tggcagaaac tgggcggcgg caccgttctg
     2581 caacaaaaat ttggttccac cagcgaatta cgcgcgggtg ttaacggcgg ttctggtatt
     2641 gctttaacgg gtagcccgat tactcccaga gcgacaaccg actccggcat gacgaccaac
     2701 aatccaacgc tgcaaaccac gccaaccgat gaccagttca ccaataatgg cggtcgtgtc
     2761 gatgcggtct acattgtggc aacgccgggt gaaatcgctt ttatcaaacc gatgatcgcc
     2821 atgcgtaacg gtagccagag cggtgcaacg ctgtacgcca gttcccgcag cgcacaaggc
     2881 accgcaggcc cggatttccg actggagatg gaaggcttgc agtacagcga aatcccgatg
     2941 ctggcaggcg gtaatctgcc gttaatgcag caggcgctca gcgcggtgaa caacgattat
     3001 tcgctggctc gcatgtatgc gatgggcgtt gatgcctggt cgttggcaaa tcatttctca
     3061 caaatgcgcc aggttcaggg ttttgaaatc aacggtaata ccggaagtct gacggctaac
     3121 ccggattgcg tgattaacag gaagttatca tggctacagt accaacaagg tcaggtagtc
     3181 cccgccagtt aaccaccaaa cagaccggcg atgcgtggga agcacaagcg cgtcgctggc
     3241 tggaaggcaa aggactgcgg tttatcgccg ctaacgtgaa cgagcgtggc ggcgagatcg
     3301 atctgataat gcgtgaaggc ctgaccaccg tttttgtcga ggtacgctat cgccgctctg
     3361 cgctttatgg cggcgcggca gccagtgtga cccgcagcaa acaacacaaa ttattacaga
     3421 ctgcccgctt gtggctcgcg cgtcataatg ggagttttga tactgtggat tgccggttcg
     3481 atgtggtagc cttcaccggg aatgaggttg agtggattaa ggatgccttt aatgaccact
     3541 cataattaag gtttaaggat tagcgtgcaa gaaagaatta aagcttgctt cactgaaagc
     3601 attcaaactc aaattgcggc ggcagaggcg cttccggatg ccatctcccg tgcagccatg
     3661 acgctggttc agtctctgct caatggcaac aaaatcctct gttgtggtaa tggaacttcc
     3721 gctgccaatg cacagcattt tgctgccagc atgatcaacc gtttcgaaac ggagcggccc
     3781 agcttacctg ccattgcact aaatactgat aatgttgtct taacggcgat tgccaacgat
     3841 cgcttacatg atgaagtgta tgcaaaacag gtgcgggcgc tgggtcatgc gggagatgta
     3901 ttgttagcca tttccacccg tggcaacagc cgcgatattg ttaaagcagt tgaagccgcc
     3961 gttacgcgtg atatgaccat tgtggcattg accggctatg acggcggcga acttgcaggt
     4021 ttgttagggc cacaggatgt ggagatccgc attccttcgc atcgtagtgc tcgcattcag
     4081 gaaatgcata tgctgacggt aaattgcctg tgcgatctga tcgataacac gcttttccct
     4141 caccaggatg attaaggaga atacatgaag gcattatcgc caatcgcagt ccttatttcc
     4201 gcgctgctgt tgcaaggttg tgttgccgct gccgtagtgg gtaccgctgc tgtgggtacc
     4261 aaagccgcaa ctgacccacg cagtgtcggc acccaggtgg acgatggtac cctggaagtg
     4321 cgcgtgaaca gcgcattgtc gaaagacgaa cagattaaga aagaaacccg tattaatgtc
     4381 acggcctatc agggcaaagt gctgctggtt gggcagtcac caaatgctga actttcggct
     4441 cgcgccaaac agattgctat gggcgtagac ggtgccaacg aagtgtataa cgagattcgt
     4501 cagggccagc cgattggtct gggcgaagca tctaacgata cgtggatcac caccaaagtg
     4561 cgttcgcagc tcttaaccag cgacctggtg aaatcgtcca acgtgaaagt gaccaccgaa
     4621 aacggtgaag tgttcctgat ggggctggtg actgaacgtg aagcgaaagc ggcggcagat
     4681 attgccagcc gggtgagcgg cgtaaagcgg gtaaccacag cgtttacgtt tattaaatag
     4741 cagccccttg taatgcctga tgcgacgctt gccgcgtctt atcaggccta caacgatgca
     4801 aaccgtaggt cggataaggc gtttacgccg catccgacat gttttccctc aaatcagaac
     4861 aacagcgcca gcccgccgac aatcacacca gacactgcca ccatcgcccc tgtcagccat
     4921 aaggctttcg ccgggaacgc tttgcgcagc ataatcagtg acggcaaact cactgccggg
     4981 agcgtcatca acaatgccag cgccggagcg gttcccatac ctgccagcat catcgtttgt
     5041 acaatcggaa tttctgctgc cgtgggaatg acaaacaagc atcctgctac cgccatcgcc
     5101 accacccaca tcaggccgtt atcgacagca ccatcggcat gggggaataa ccagacgcgt
     5161 gcggcaccca acaccagtac tgcaaggatg taaaccggga tcgtactcca gaaaagtgtc
     5221 catagcgccc tgccccagcg gctaaaaaaa ccgctctgtg cttccggtat gtcaatttcg
     5281 accggtgcct gcgtttgcgg tgtttcacgc acccattttt gcaccagcgt cgcaatcagc
     5341 aacaccatca ccagcccggc caccagacga atcgccgcaa aaccccagcc gaggacaaac
     5401 cccataaaca ccagcgtcgc cgggtttaac accggattgc ccatccagaa tgccagcgca
     5461 ccgcccatcg atacctgttg gcgacgcatt cccgccgcga ccggagccgc acagcaggta
     5521 cacatcatgc ccggcagcga aaacagcgtt cccagcagcg tgccgcgaaa gcgcgattgc
     5581 ccaagcgtac gcaacaacca gtcacgcggg atcagcacct gaatcaacga accgaggatc
     5641 acccccagca ccgccgcttt ccatactgcg aggaaataga tcatcgcgta atccaacgcc
     5701 gcttgccacg gattagcatc cgcctgcgca aggatagatt taccgatact gtgggtttcg
     5761 gctgcagtaa acgctttgcc gtagtaaggt tgccatttca cataccagag accggcaatg
     5821 acaacgagaa agaaaagcgc gggtttccac cactgaatgg gcgttgccgc ctgagatgaa
     5881 gactgaccag tcatagcatt ccccaggaat aagttatgat aattgagcgc gtgaatatta
     5941 cgctcactat caattcttgg gaataattat tcagctcttt tgcgtaattc tgaagagctt
     6001 aaaatcgtca cgccttcatg ctcaggccgc atcgcctctg ccagcatgac ccgcgccacg
     6061 tctctcgcat caatggattt ccagttacct ggtaacaaac ggaacagcgg cgcaaaaagc
     6121 gtttcgttca tccgctgttt gctacgatcg cccagtaaca tcgacgggcg agcaatggtc
     6181 aatttcggcc agttctgggc aattaatgct tcttccatct cccctttgac gcggttatag
     6241 aaaaacggcg agtgggcatt ggcacccata gcactgacca ccagcatatg ctgcgcgccc
     6301 agtcgccgcc cggttaatgc ggtatccact accagcgtgt aatcggcatg aataaacgcc
     6361 tctttgctcc ccgcttctcg ccgcgtggtg ccgagacaac aaaacacaat gtcgatgggt
     6421 tcggtgacct gcgccaacgc gtcactcagt tgcggatcat ggggattaaa caccccgggc
     6481 atatcgccca acggacgtcg cgtcggcgca gcaatggcgt taactttcgg ttcattaatc
     6541 aacatccgca gcaggtgacc gcccaccagc cccgttgcgc ctgtaatcag tacctgactc
     6601 atcttcgctc ctttacagaa ttgtctgcct tgcgctcccc ggctcatgca ccagtcttaa
     6661 tagaccatga ggtaattatc cccgattgtg gaaaattcgc ctcatccaat gcaacaacgc
     6721 ggaggaagca tgagtaagaa aattgccgtt ttaatcactg atgaatttga agattcagaa
     6781 tttacttcac ccgcagacga gttccgtaaa gccggacacg aagtgattac tattgaaaaa
     6841 caagcgggta aaacggtgaa aggcaaaaaa ggagaagcca gcgtgaccat cgataaatcc
     6901 atcgatgaag tgacgtcagc ggagtttgat gccctgttgc taccgggtgg tcattcaccg
     6961 gattatctgc gtggcgacaa ccgttttgtc acctttaccc gtgattttgt gaatagcggc
     7021 aaacctgttt tcgccatctg tcacggtccg cagctgctga tcagcgccga tgtgattcgg
     7081 gggcgcaaac tgaccgcagt taaaccgatc attattgatg ttaaaaatgc gggcgtggaa
     7141 ttttacgatc aggaagtcgt ggttgataaa gatcagctgg ttaccagccg gacaccggac
     7201 gatctgccag cgtttaaccg cgaggcgtta cgcctgctcg gtgcctgagt cgcgtagcca
     7261 aatcattttt ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat
     7321 ttcccacacc ggggccgaca gcattctggc aaccggaaag cgacgattgt acgccttgcg
     7381 cgcgaggtcg ctttcctcac cttccagcct gcggatctca cctttaaact gcacaccgcg
     7441 aattaacgct accgttttcg gctgaccgtt taccgttccg gcaaccgcag cctgcggccc
     7501 actcatctgc gcgtggcgcg ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc
     7561 atcaaaaaga taaaaggcat tagcgcacca gagttcccct tcctgctgca cacaccaggt
     7621 gacaacatgt tgttttgcca gccaacggct gatggcgatg agtgtttcca ttgctgttct
     7681 cttttatact gtgggcctga ccttaacatg cgatgcagac gatgaccccc tggtttcttt
     7741 acttgatccg taccgccgac aataagcttt ataccgggat caccacggat gtcgaacgcc
     7801 gctatcagca gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaaa ggagaactga
     7861 cgctggcgtt ttctgcgcca gtgggcgacc gttcgctggc actacgggcg gaatatcgcg
     7921 ttaagcaact gaccaaacgg caaaaagagc gtctggtagc ggaaggcgca gggtttgcag
     7981 aactgttgag cagcctgcaa accccggaga ttaaaagcga ttgaaatgct cgtgatactc
     8041 aaccagaccg gttacgccat tcagcgcgtc atccgctaaa cgatgtacct ggaaggcgct
     8101 ttcagtgccc ggccagcggc aacgcagatc gtgatgcgcc gccagttcaa agccgaaacg
     8161 actgtacagc gccggatcgc ccagcgtcac cactgcggca tagccgaact cattaagcga
     8221 atcgagtcct tcatagacca gttggcgtgc cagcccctgt ccacggtatt tttcatcaac
     8281 cgccaacggt gccatgccga cccattgcag gtcttcgccc tgcacatcaa ccggactaaa
     8341 tgccacatag ccaatgacct gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa
     8401 gccatcttca cgcagatcgt gaaccagctt cgcttccgca tcactttcga atgagcgacg
     8461 cagcagggca tcaatacccg gcgcatcaat gggaatttct actcgaatta gcatggttca
     8521 cctaccgatg tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc
     8581 agttgcagca gcatcatgcg taaggctttc ggcatttgct ccagttcaat ggcgtccatc
     8641 aggtttttca catacacccc cagctccgta tcgccttcaa tcaccagccg acgctggaag
     8701 aagagcgtat ccggatcttg tttacgcgcc gcaatcatca gcagatcgct ggcgtcggca
     8761 ctaaaactca catcagcttg cgcgttctgg ctgaccacca gtttgccatt caccaccgag
     8821 gtaaaccatt gcaggtcaat atcacgcaca tgaatactta accagcggcc ttcaagaaca
     8881 tcatccagcg cctgacggaa ttgccagctt aagacctgct caagaacctg gcgttttagc
     8941 gcaaatggcg tcagttttac cggtacactc aacagagatg gccccaaatg cacaatacgg
     9001 gaacgcagtt tatccaacac gagttttact ccctgtttca acaatcatcc tattttgcca
     9061 tatcagaaaa ataacatagc ggtataaatc aacaattcca tatgaaattg ctgctaccac
     9121 caatacaact ttaactgcct taaatcaaaa attgtcgcag caaggttaac taaaatccca
     9181 gttcgttaac atttttgcgt tttgatagcg caaccttcag gaaaaattat ggagctgctc
     9241 tgccctgccg gaaatctccc ggcgcttaag gcggccatcg aaaacggcgc agatgctgtt
     9301 tatatcgggc taaaagatga taccaatgcc cgtcacttcg ccggccttaa ctttaccgag
     9361 aaaaaattgc aggaagcggt gagttttgtc catcaacatc gccgcaaact tcacatctcg
     9421 attaacactt ttgcgcatcc ggacggttac gcccgttggc agcgtgccgt ggatatggcg
     9481 gcgcagctgg gtgccgacgc gctgatcctc gccgacctcg ccatgctgga gtacgccgcc
     9541 gagcgttacc cgcatattga acgtcatgtg tcagtgcagg cttcggcgac caatgaagag
     9601 gcgattaact tttatcatcg ccattttgac gttgcccgcg tggtgctgcc gcgcgtgttg
     9661 tcgattcatc aggtgaaaca gctggcacgg gttactcctg tcccgctgga agtgtttgct
     9721 ttcggcagcc tgtgcattat gtcggaaggc cgttgctatc tgtcgtcgta tctgacgggt
     9781 gagtcgccca acactgtggg tgcgtgttct ccggcccgtt tcgtgcgctg gcaacaaacg
     9841 ccgcaggggc tggaatcccg cctgaacgaa gtgctgatcg accgttatca ggacggcgaa
     9901 aacgcaggtt atccgacgct gtgtaaaggg cgttatctgg tggacggcga gcgctatcac
     9961 gcgctggaag aaccaaccag tctcaatacc ctggaactgc tgccggagtt aatggctgcg
    10021 aatattgctt cggtgaaaat tgaaggccgc cagcgtagcc cggcgtatgt cagccaggtg
    10081 gcgaaagtct ggcgtcaggc tatcgaccgt tgtaaggccg atccgcaaaa cttcgtaccg
    10141 caaagcgcgt ggatggagac gctcgggtcg atgtccgaag gcacgcaaac cacccttggc
    10201 gcgtatcacc gtaaatggca gtgagaagag caatgaaata ttccttaggg ccagtgctgt
    10261 ggtactggcc aaaagagacg ctggaagatt tttatcagca agccgccgcc agcagcgccg
    10321 acgtgattta tcttggtgaa gcggtatgca gcaagcgccg ggcaaccaaa gttggcgact
    10381 gtctggagat ggcaaaatcg ctcgccggaa gtggcaagca gattgtgctc tccacgctgg
    10441 cgctggtgca ggcatcctct gaactgggcg aactgaaacg ctatgttgag aacggtgagt
    10501 ttctgattga agccagcgat ctcggcgtgg tgaatatgtg cgctgaacgc aaactgccgt
    10561 tcgtcgccgg gcacgcgctg aactgctaca acgcggtgac gctgaaaata ttgctcaaac
    10621 agggcatgat gcgctggtgt atgccggtgg agctttcccg cgactggctg gtgaatctgc
    10681 ttaatcagtg cgatgagctg ggcattcgca accagtttga agtggaagtc ctgagctacg
    10741 gtcatctacc gctggcctac tccgcccgct gctttaccgc gcgttcggaa gaccgcccga
    10801 aagatgagtg tgaaacctgc tgcattaagt atccgaacgg gcgcaacgtg ctgtcgcagg
    10861 aaaaccaaca agtgtttgta ctcaatggca ttcagaccat gagcggctac gtttacaacc
    10921 tcggtaacga gctggcatcc atgcagggat tggttgatgt agtccgcctg tcaccgcagg
    10981 gcactgacac tttcgcgatg ctcgacgcct tccgcgctaa tgaaaatggc gctgtgccac
    11041 tgccgttgac ggcaaacagc gactgtaacg gctactggcg gcggctggca ggactggagc
    11101 tgcaagccta agtaaatagc tcactttgtt aacaacttta actattcttt aatgcagtat
    11161 taaagattaa tcggtaacaa agtgagctgt tatgactgat aaaaccattg cgttttcgct
    11221 actcgatctg gcccccatac ccgaaggctc ttcagcgcgg gaagcgttct cccactctct
    11281 cgatctcgcc cgtctggctg aaaagcgcgg ctatcatcgc tactggctgg cagaacacca
    11341 caatatgacc ggcattgcca gtgctgcaac atcggtattg attggctatc tggcggcgaa
    11401 taccaccacg ctgcatctgg ggtctggcgg cgtgatgttg cctaaccact caccgttggt
    11461 cattgccgaa cagttcggca cactcaatac gctctatccg gggcgaatcg atttggggct
    11521 gggtcgtgct ccgggtagtg accaacggac aatgatggcg ctacgtcgtc atatgagcgg
    11581 cgatattgat aatttccccc gcgatgtcgc ggagctggtg gactggtttg acgcccgcga
    11641 tcccaatccg catgtgcgcc cggtaccagg ctatggcgag aaaatccccg tgtggttgtt
    11701 aggctccagc ctttacagtg cacaactggc ggcgcagctt ggtctgccat ttgcgtttgc
    11761 ctcacacttc gcgccggata tgttgttcca ggcgctgcat ctttatcgca gtaacttcaa
    11821 accgtcggct cggctggaaa aaccatacgc gatggtgtgc atcaatatta tcgccgccga
    11881 cagcaaccgc gacgctgaat ttctgtttac ctcaatacag caagcctttg tgaagctgcg
    11941 ccgtggcgaa atcgggcaac tgccgccgcc gattcaaaat atggatcagt tctggtcacc
    12001 gtctgagcag tatgacgtgc agcaggcgct gagtatgtcg ttggtaggcg ataaagcgaa
    12061 agtgcgtcat ggtttgcagt cgatcctgcg cgaaaccgac gctgatgaga ttatggttaa
    12121 cgggcagatt ttcgaccacc aggcgcgcct gcattcgttt gagctggcga tggatgttaa
    12181 ggaagagttg ttgggatagt gtgtcttaac gcgggaagcc ttatccgagc tggcaacgct
    12241 gtcctacgta ggcctgataa gcgaagcgca tcaggcattg tgtaggcagc agaaatgtcg
    12301 gataaggcac cgctgattac tgatacaccg gcagtaaatt aaagctcgat aaaatatgca
    12361 ccagtgcgtt gccgacgcca aacaccagaa tcagcgcaat catcggcttg ccaccccaga
    12421 cgcggaattt cgggctgcca aagcgtttac gcgatgcgcg ggccagcagt gccggaacaa
    12481 ttgccgccca gatggtagcc gctaaaccag cataaccaat ggcatacagg aatccgttcg
    12541 ggaacagcag accacccaca acaggcgggg caaaggtcag caatgccgtt ttcaagcggc
    12601 ccacagccga gtcgtcgaaa ccaaacagat ctgccagata gtcaaacaaa cccagcgtta
    12661 cgccaaggaa cgaactcgct accgcaaagt ttgagaacac gaccagcaga aggtccaggc
    12721 tacggctgtt cagcacgccg cttaatgctt gtaccagcac atcaatatta ccgcccttct
    12781 cggcgatgcc gataaactcc ggacgaggga tgttgcccat tgtcgccagc aaccagatgg
    12841 tatacagcgc cagtgccatc agcgtaccgt acaccaggca tttcacgatg gttttcggat
    12901 ctttgccgta atacttcatc aggcttggca cgttaccgtg ataaccaaac gacgccaagc
    12961 agaacggcag ggtcatcagc aaatacggcg cataagatgc attgctttcg gcaacgttga
    13021 acaatgtcgc aggctgcaca tgccccagca ggctaccgaa ggtgaggaag aaggtaatga
    13081 ctttcgcccc cagcacaatc gccgtcatgc ggctgacggc tttagtactc aaccacacca
    13141 caaacgctac cagcagtgca aaaccaaaac ccgccgcccg tgccgggacg tttagcgaca
    13201 tctctgcgaa ggtgtgatgc agaatcgaac cactggcaga aatataggca taggtcagga
    13261 tatagagcac aaaggcaatg gaaatgccgt tgaccacgtt ccagcctttg cccagcaaat
    13321 ctttggtgat ggtgtcaaaa ctcgaaccga ttcggtaatt caggttagct tccagaatca
    13381 tcaagccgga atgcagcata cagaaccagg taaagatcag cgctgccatt gaccagaaaa
    13441 accacgcccc ggacatgacc actggcagag aaaacatccc tgcgccaata atggtgccgc
    13501 cgataatcac cacgccgcca agcagcgacg gtgacgtttg ggtggtggtt agtgttgcca
    13561 tgagggcttc tctccagtga aaaatagtgc gactgcgttg ttatgcattg cactgtacca
    13621 gtacatgagt acaaaagaca gaaaaaaagc cccgatggtt aaaatcgggg ctgtatatat
    13681 tattttacag attgtgttcg ctgttcagcg atgattacgc atcaccaccg aaacgacgac
    13741 gaccggtaga gtcatcacga cgcggtgcgc ggccttcacg acgttcgccg ctaaaacgac
    13801 gaccatcacc acgaccacct tcacggcgtt caccgctgaa gttacgaccg ccttcacgac
    13861 gctcgccacc gaaaccacga ccaccgccac gaagctcacc gccagtatgc ggctgtgcat
    13921 cgcccagtaa ctgcatgttc atcggcttgt tgagaatgcg agtgcgcgta aagtgttgca
    13981 gcacttcacc cggcatacct ttcggcagtt cgatggtgga gtgagaagca aacagcttga
    14041 tgttaccgat gtaacggctg ctgatgtcgc cttcgttagc aatcgcgcca acgatatgac
    14101 gaacttcaac accatcatcg cggcccactt caatgcggta cagctgcata tcgccaacat
    14161 cacgacgttc acgacgcgga cgatcttcac ggtcgccacg cgggccacgg tcgttacgat
    14221 cgcgcggacc acggtcatca cggtcacgga attcacgttt cggacgcatc ggcgcatctg
    14281 gcggaacaat cagagtacgt tcaccctgtg ccattttcag cagtgccgca gccagagttt
    14341 cgagatccag ctcttcacct tctgcagtcg gctgaatttt gctcagcagt gcgcggtatt
    14401 gatccagatc gctgctttcc agctgctgct gtactttagc ggcgaatttt tccagacggc
    14461 gtttgcccag cagttctgcg ttcggcagtt ctacttccgg aatagtcagc ttcatagtac
    14521 gttcaatgtt gcgcagcaga cgacgctcgc ggttctcaac gaacagcagc gcgcggccag
    14581 cacgacccgc acgaccggta cgaccgatac ggtgaacgta agactcagaa tccatcggga
    14641 tatcgtagtt aaccaccagg ctgatacgct caacgtccag gccacgggcc gcaacgtcgg
    14701 tcgcaatcag gatgtccaga cgaccgtctt taaggcgttc cagtgtctgc tcacgcagcg
    14761 cctggttcat gtcaccgttc agcgcggcgc tattgtagcc gttacgctca agagcttcag
    14821 ccacttccag agtcgcgttt ttggtacgaa cgaagataat cgccgcatca aaatcttccg
    14881 cttccaggaa acgtaccagt gcttcgtttt tacgcatacc ccacacagtc cagtagctct
    14941 ggctgatgtc aggacgggta gtcacgctgg actgaatacg cacttcctgc ggctctttca
    15001 taaagcggcg ggtaatgcga cgaatcgctt ccggcatggt tgcagagaac agagcggtct
    15061 gatgaccttc cgggatctgc gccataatgg tttcaacgtc ttcgatgaag cccatgcgca
    15121 gcatttcgtc agcttcatcc agaaccagac cgctcagttt agagaggtcc agagtgccac
    15181 gtttcaggtg gtccagcaga cggcccggag taccgacaac gatctgcggc ccctgacgca
    15241 gggcgcgtaa ttgcacgtca taacgctggc cgccgtacag ggcaaccaca tttacgccgc
    15301 gcatgtgttt agagaaatcc gtcattgctt cagcaacctg taccgccagt tcgcgggtcg
    15361 gtgccagcac cagaatctgt ggtgctttca gctcaggatc aagattctgc agcagaggta
    15421 aagagaatgc tgcagttttt ccgctccccg tctgggccat acccagaacg tcgcggccat
    15481 tcagcagatg tggaatacac tctgcctgaa ttggagatgg tttttcgtaa cccagatcgt
    15541 taagggcttc aaggatagga gccttcaggc ccagatctgc aaaagtggtt tcgaattcag
    15601 ccatgtagta cgtgtgcctc aaaattaatg gcggccagtc tacataactc atcatgaaat
    15661 tgatcagcaa ttttcattga aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa
    15721 caacgccctc acccgttaag gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt
    15781 cagctattgc tggtccgatt ctgccaggtc atcttggtcc tggcccagga gcgataattc
    15841 caacaatgcg tatcggtgct caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa
    15901 cagtgccgtg gcgctgtcca aatcccccag acttaggtag tacttaccta aatagaagtt
    15961 ggtttcactg agatgctcag cgagcgaggt gttatcggtt gcgtccgcct tgagcctttc
    16021 cattaacgtt tgttcgctaa tgttgcccag gtagaactcg acaatgttcc atccccactg
    16081 ttccttatcc gatttttcga agtgctgttt caacacttct ttagcctgct tctcatcgag
    16141 cttctgctcg gcgagataaa gccacagact acggaaagga tcattgggat cgtcttgata
    16201 aaacgccagc agatcatctt gcgctaactt gtcacgaccg ccgtaatata atgcaatccc
    16261 gcgattcaag tgcgcgtagt tgtaagttgg atcaagctca agtacagaat caaacgcttc
    16321 ataggcagca tcaaaattgc ctgcctgcgt taaatatatg cctaagtaat tgaatacttc
    16381 aggcatatcc ggtcggattg ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag
    16441 accgagacta tcatacaaca ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt
    16501 taaagcccga ctggcaagga tttgttccat acgtgccaga atcacttcct gctgtaaagt
    16561 cggttgcaat ggtaccgcga ggacttcact tttacgccag gaagtattac tgcatcctgc
    16621 aagcgtaagt gctgtcgcaa cgaaacacca gcgcaaaaaa ggcttcattt cccactcccg
    16681 aagaccacgg ttgaatgaac gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta
    16741 aaagcccccc gccgcagcgg agggcaaatg gcaaccttac tcgccctgtt cagcagccgg
    16801 agcttccggt gctgcagcag gttgagactg ctcagtcgct tctttaatgc tcagacggat
    16861 acggccctgg cgatcaactt ccagaacttt caccggtact tcctgaccca tctgcaggta
    16921 atcggtcact ttctcaacgc gtttgtcagc gatttgagag atgtgtacca gaccttcttt
    16981 accgccgccg atggcaacaa atgcgccaaa gtcaacgata cgggtcactt taccagtgta
    17041 gacgcggccc acttcgattt ctgcagtgat ctcttcgata cgacgaatag catgtttcgc
    17101 tttctcgccg tcggtcgctg caatcttcac agtaccgtca tcttcgattt cgatggtggt
    17161 gccagtttct tcggtcaggg cacggataac agagccgcct ttaccgataa cgtctttgat
    17221 cttgtccggg ttgatcttga tggtatggat acgcggtgcg aactcagaga tatcgccacg
    17281 cggcgcgttg atcgcctgtt ccattacgcc caggatatgc agacgcgcac ctttagcctg
    17341 gttcagcgca acctgcatga tctctttggt gataccttca attttgatat ccatctgcaa
    17401 tgcagagata ccctcgcggg aacccgcaac tttgaagtcc atatcgccca ggtgatcttc
    17461 gtcgcccaaa atgtcagaca gtacaacgta gttgtcgcct tctttcacca gacccattgc
    17521 gatacccgca acggcagctt tgatcggcac acctgcgtcc atcagcgcca gagacgcgcc
    17581 gcacacggaa gccatagaag aggaaccgtt ggattcagtg atttcagaca caacacgtac
    17641 ggtgtacggg aatttgtcca tatccggcat gactgccagc acgccgcgct tcgccagacg
    17701 accgtgacca atttcacgac gcttcggaga accgaccatg ccggtttcgc ctacggagta
    17761 cggagggaag ttgtagtgga acaggaaggt atcggtacgt tcgcccatca gttcatcaag
    17821 aacctgcgcg tcacgagcag tacccagcgt tgcggtaacc agcgcctgcg tttcaccacg
    17881 ggtgaacagc gcagaaccgt gagtacgcgg cagcacgcca gtacgcacat ccagaccacg
    17941 gatcatatct ttttcacgac cgtcgatacg cggttcgcct gccagtacgc ggctacgaac
    18001 aacgtttttc tcgatcgcgt gcagaatttc acccagttcg ttttcgtcca gggtttcgtc
    18061 ttcagcaagc agcgtcgcga tggtttcaga tttgatgaca tcaacctgcg cataacgctc
    18121 ttgtttgtcg gtgatgcggt aagcatcgct caggcgagct tcagccagtg cagcaacgcg
    18181 cgcgttcagc gcttcgttta ccggctccgg ctgccagtcc caacgtggtt taccggcttc
    18241 tttcaccagt tcattgatgt tctgaataac aacctgctgt tgttcatgac cgaacactac
    18301 tgcgcccagc atctggtctt cgctcagcag ttcagcttca gattcaacca tcagtacagc
    18361 ggcttcagta ccggcaacaa ccagatccag tttgctctct ttcaactcgt cctgagtcgg
    18421 gttcagtacg tactggtcat tgatgtaacc tacgcgagca gcaccaatcg gaccattgaa
    18481 cggaatacca gacagagaca gtgctgcgga agcaccaatc atcgcgacga tatccgggtt
    18541 aacttgcggg ttaacagaaa ccacggtggc gataacctga acttcgttga cgaagccttc
    18601 cgggaacagc gggcgaatcg ggcggtcaat cagacgcgcg atcagggttt cgccttcgct
    18661 tgggcggcct tcacgacgga agaagctacc cgggatacga ccagcagcgt aggtacgctc
    18721 ctgatagtta acggtcagtg ggaagaagtc ctgacctggt ttggcttttt tctggccaac
    18781 aacggtaaca aataccgcgg tgtcatccat gctaaccata acagcggcag tagcctgacg
    18841 agccatcatg ccggtttcca gagtcacggt gtgttggccg tactggaatt tacgaacgat
    18901 cggattaagc aatgtaatat cctttctctt tcttagacag taccttacgg cactggtgtt
    18961 aatacccgat cttctgcgca tcctcgcgac taatgacaac cctaacccag ctctatgtgg
    19021 gtaaagcctc tcattagccg cgcgaacctc tgcaacggaa gatcattcat agcaacaata
    19081 cattagtttc cagtgaattg ctgccgtcag cttgaaaaaa ggggccactc aggccccctt
    19141 ttctgaaact cgcaagaatt agcgacgcag acccaggcgc tcgatgagct gggtgtaacg
    19201 tgctacgtct ttacgtttca ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag
    19261 cagaccacga cggctgtggt gatctttttt gtgctctgca aagtggccct gcaggtggtt
    19321 gatctgtgca gtcagcagtg ctacctgaac ttcagtagaa ccggtgtcgt ttgcgtcacg
    19381 accaaactca gaaacgattt tagctgttgc ttcagtactt agagacattt taaaactcca
    19441 aagtatatag aatgaaagga cgccgatctc taattcagcg atcccagtgt acgttacgca
    19501 aagtgttaaa taatttacgc gacgttaagc ggcagtattc tactcgtagc gacctgttat
    19561 cgcaagacgg ttaacattac gccgggtatt caaccaccag gcgacgaggc gcaacgcggc
    19621 cttcatcgtc aatttcgccc ataccgataa atttgccgtt ctcaccttct gtaacgcgaa
    19681 ccagtccttc cagcggcgca ccggatgtac gaactgggtt accatttttg aagtaaacag
    19741 aagacgttaa cggaagattc accaccgggt agtccgaagc tggactgtcc attggcatca
    19801 gtaatggatc aagtaactcc gcagccggaa tatcctgctg ttcggcttgt tcgaccagtt
    19861 cacgcagatg ttccagggtc acaatccgtt caaccggata tttacttacc gccagacgac
    19921 gcaggtaaat aacatgcgcg ccacaaccga gtttttcacc caggtcatca atgatggtgc
    19981 ggatataagt gccttttgag cagtgaattt ccagctccag ctcattgcct tcgtggcgaa
    20041 taaacagcaa ttcataaacg gtaatcgggc gcgcttcacg cggtacttca atgccctgac
    20101 gcgcatattc gtacagtttt ttaccctgat atttcagtgc tgaatacatc gaagggatct
    20161 gttcgatatc gccacggaaa gtatccagcg ccgccgccag ttgctctgcg ctaaaggtta
    20221 ccggacgttc ttcaacgatt tgtccgtcgg catcagaagt atcggtacgc tgtccaagac
    20281 gcgcaatgac ccgatagcgt ttgtcggagt ccagcagata ctgggaaaac ttcgtcgctt
    20341 ccccgaggca aattggcaac atgccagttg ccagcgggtc cagcgcaccg gtatgcccgg
    20401 cacggttggc gttataaatc cgtttcactt tttgcagcgc atcattgctg gacataccct
    20461 gcggtttatc cagcaacaaa acgccgttaa tgtcgcgacc gcgacgacga ggacgactca
    20521 ttagtcctcc ttgctgtcgt ccgggttaac acgacgttct tcgtcatgtt tgaccacgct
    20581 ggtcaccagg tttgacatgc gcatcccttc aaccagagag ttgtcgtaga agaaggtcag
    20641 ttccggcacg atacgcaggc gcatcgcttt ccccagcagg ctgcggatga aaccagaagc
    20701 ttcttgcaac gctttgatgc ccgctttaac cgcgtcttca tctttgtcgt tgaggaacgt
    20761 cacatatact ttggcatacg ccaggtcgcg agacatttcg acaccggaaa cggtggtcat
    20821 catgcccagg cgaggatctt taatttcacg ctgcaggatg agagcgatct ctttttgcat
    20881 ttcctgcgct acgcgctgcg ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa
    20941 aaaaggggct gttagcccct ttttaaaatt aatttcaggt ggaagggctg ttcacgttgg
    21001 cctgataaga cgcgccagcg tcacatcagg caatccatgc cggatgcggc gtaaacgcct
    21061 tatcccgcat ggaaccctaa aaaccttaag cgatggtacg ttggatctcg atgatttcga
    21121 atacttcgat cacatcgcca gtgcggacgt cgttgtagtt cttaacgccg ataccacatt
    21181 ccataccgtt acggacttcg ttaacgtcat ctttgaagcg gcgcagggac tccagctcgc
    21241 cttcgtagat aaccacgttg tcacgcagaa cgcggatcgg gttgtgacgt ttaaccacac
    21301 cttcggtaac catacagcct gcgatggcac caaatttcgg cgatttgaac acgtcacgaa
    21361 cttccgccag accgataatc tgctgtttca gttccggaga cagcataccg ctcatcgccg
    21421 ctttcacttc gtcaatcagg ttatagatga cggagtagta acgcagatcc aggctttccg
    21481 cttcaatcac tttacgtgca gaggcatcag cacgtacgtt gaagccaacc aggatggcgt
    21541 tggacgccgc agccagggtg gcgtcggttt cggtgatacc acctacgcca gaaccgatga
    21601 tcttcacttt aacttcgtca gtagacagtt tcagcaagga gtcggagatc gcttccacag
    21661 aaccctgtac gtctgccttc aggacgatgt tcacttcgtg aacttcgcct tcggtcatgt
    21721 tggcgaacat gttctcgagt ttagatttct gctgacgcgc cagtttaact tcgcggaatt
    21781 taccctgacg atacagtgca acttcacgcg ctttcttctc gtcacgtaca acggtaactt
    21841 catcacccgc agccggtacg ccggacaggc cgaggatttc caccggaatg gacggacccg
    21901 cttccagcac ttcctgaccc agttcgttac gcatcgcacg aacacgaccg tattcgaagc
    21961 cacacagaac gatatcgccc ttgtgcagag taccttcacg taccagaacg gtagcaaccg
    22021 gaccacgacc tttatcgagg aaggattcga taaccgcacc gctcgccata cctttacgta
    22081 ccgctttcag ctccagaact tccgcctgca gcaggatagc gtccagcagt tcatcgatac
    22141 cagtacccgc tttcgcagac acatgtacga actggctttc accgccccac tcttccggca
    22201 ggatgccgta ctgggagagt tcgttcttaa cgcgatccgg atcggcttct ggtttatcga
    22261 tcttgttcac tgcaacgacc accggaacct gcgccgcttt cgcgtgctgg attgcttcga
    22321 tcgtctgcgg cattacgccg tcgtcggcag caacaaccag gactacgatg tccgttgcct
    22381 gcgcaccacg agcacgcatt gaagtaaacg cggcgtgccc cggggtgtcc aggaaggtga
    22441 tcatgccgtt ttcagtttca acgtggtatg caccaatgtg ctgagtaatg ccgcccgctt
    22501 cgccagaggc cactttcgtt gaacgaatgt agtccagcag agaggtttta ccgtggtcaa
    22561 cgtgacccat gatggtcaca accggcgcgc gcggttcagc cgcagcaccc gtgtcacggt
    22621 cgctcattac cgcctcttcc agctcgtttt cacgacgcag gataacttta tggcccatct
    22681 cttcagcaac cagctgtgcg gtttcctgat cgataacctg gttgatggtt gccattgcgc
    22741 ccagtttcat catcgctttg atgacctgag agcctttaac cgccatcttg ttcgccagtt
    22801 cgccaacggt gatagtttcg ccgatcacaa cgtcacggtt aacggcctga gcaggcttct
    22861 ggaagccttg ctgcagcgaa gaacctttac gttttccgcc tttaccgcca cgtactgctg
    22921 cgcgtgcttc ttcacgatca gcttttgatt cagcgtgttt gttgcctttc ttcggacgcg
    22981 ctgctttcgc gttacgacca cggccacggc cgccttcgac ttcacgatcg ctttcgtctt
    23041 ctgcctggcg agcatgttga gaagtagtga cgtgataatc gctggaatct tcagtcggtt
    23101 ccgcgttatc agtccatttg ttttcttccg ccatacgacg tgcttcttca gcaacgcgac
    23161 gtgcttcttc ttcgagttta cgacgcgctt cttcttcagc tttacgcttg agctctgcag
    23221 cttcctgctc acggcgggct ttttcagcct gggcgttttt agtcatatcg tcttgttgat
    23281 tgctcacttt gtctttttcc gcagcttcac gtttcgcttg ttcagcagct tcacgcttag
    23341 cttgttctgc ggcctcacgt tcagcttttt gttgcgcctc gcgtttagcc gattcttctg
    23401 cctcacgacg ggcttgctct tccgctgcaa ggcgttcagc ctcttgcgga tcgcgtttca
    23461 caaaggtgcg tttcttgcgg acttcgattt gtaccgattt gctttttcca ccggtaccag
    23521 gaatgttaag ggtgctgcgt gttttacgtt gcagcgtcaa tttgtccggg cctgaatttt
    23581 tctgattcag gtggtcaatc aaagtctgtt tctcttgtgc agacacagag tcgtcagcag
    23641 acttccggat acctgcatca gcaaattgct gtaccaggcg ttccacggag gtctgtcgct
    23701 ctgcggccag cgttttaatc gttacatctg tcatgctgtt ccttcctgct acagtttatt
    23761 acgcttcgtc accgaaccag caaatattac gggcagccat aatcagtgct ccggcttttt
    23821 cgtcggtcaa cccttcgata tcagccagat catcaatgcc ctgttcggcg agatcttcca
    23881 gcgtacaaac gccacgggcg gccagtttga atgccaaatc acgatctacc ccttcaaggt
    23941 tcagcagatc gtcagccggt ttgttatcac cgaggctttc ttcctgggcc tgtgcaatgg
    24001 tggccagtgc atttttagca cgctcgcgca gtgcttcaac ggtcggctca tcaaggcctt
    24061 cgatttccaa cagctctttc atcggcacat aggccagttc ttccagcgtc gagaaacctt
    24121 cttctaccag aacagtcgcg aagtcttcgt cgatgtcgag atatttggtg aaggtgtcga
    24181 tcgctgcgtg cgcttccgcc tgatgcttag cctgcaggtc gtcaacggtc attacgttga
    24241 gttcccaacc gctcagctgc gaagccagac gcacgttctg accgttacgg ccaatcgcct
    24301 gcgccaggtt accggcttca acggcgatat ccatggtgtg tttatcttca tccaccacga
    24361 tagaagcaac gtctgccggt gccattgcgt taatcacaaa ctgcgccggg ttatcatccc
    24421 acaggacgat atcgatacgc tcgccgccca gttcagtaga aaccgcctga acacgcgcgc
    24481 cacgcatacc tacgcaagca cctaccggat cgatacgttt atcgttggtt ttcaccgcga
    24541 ttttcgcacg agaacccggg tcgcgagccg ctgctttaat ttcaatcact tcttcgccga
    24601 tttccggcac ttcaatacgg aacagttcga tcagcatttc cggcttggaa cgagtgacga
    24661 acagttgcgc gccacgcgct tccgggcgaa cggaataaag cacgccacga acgcggtcgc
    24721 cagggcggaa gttttcacgc ggcagcatat cttcgcgcag gatcacggct tcagcattgt
    24781 tgcccagatc cagagagatg ttgtcgcggt ttactttttt caccacgccg gtgatgattt
    24841 caccttcgtg ttcacggaac tgatcaacca ccatcgcacg ttcggcttca cgcactttct
    24901 gcacgataac ctgttttgcc gtctgggtgg tgatacggtc aaaggtaaca gactcaatct
    24961 gatcttcaac gtaatcgccc aagttcaggc tttcatcttc ataacgtgcg gcttcaaggg
    25021 tgatttcctt ggtcggctgg gtgacttcat caacaactaa ccagcgacgg aaggtgtcaa
    25081 aatcaccgct tttgcgatcg atctgtacac ggacgtcgat ctcttgctca tattttttct
    25141 ttgttgctgt cgccagcgcg ctttccaatg cttcgaaaat cttctcgcga ggtagcgcct
    25201 tttcattgga tacggcttca actacagcca aaatttcttt gttcatcgcg ggcttttcac
    25261 ctcatccaga ctattaaaag tggggaacca ggttcgcctt ctggatatta ctcagcgcga
    25321 acacttcatc tttaccttcg acggtaactg tgatcatttc accgtctacc gctttgataa
    25381 cgccctgcca tttacgacgg ttttgtaccg ccatacggag aaccagagtc acctcttctc
    25441 cgacaaaacg ggcgtagtgt tcagccgtga acagtgggcg atcgagaccc ggtgaggaga
    25501 cttccaggtt ataagcaacg gtgatgggat cttcaacatc cagcacagca cttacctggt
    25561 ggctcacatc agcacaatca tcaacattga tgccatcttc actatcaata tagatgcgca
    25621 gtgtggatgt gcgaccgcga ataaattcga tgcctaccag ttcaaaaccc agggcctcaa
    25681 ctggcgcagt aatcatctct gttaattttt gctctaatgt ggacaagccc acccccaaga
    25741 cataaaaaaa gggcctaaag cccagttatt ctgtagtcag ataacaaaaa accccgataa
    25801 atcggggctt tatataactg aaccctataa ccgcaactgc ggtctggagc actttccaga
    25861 aggatttttt caaatcccac tacgaaggcc gaagtcttca cagtatattt gaaaaaggac
    25921 tctaagggaa agtggttgcg ggggccggat ttgaaccgac gaccttcggg ttatgagccc
    25981 gacgagctac caggctgctc caccccgcgc ctgaaacgtg gcaaattcta ctcgttttgg
    26041 gtaaaaaatg caaatactgc tgggatttgg tgtaccgaga cgggacgtaa aatctgcagg
    26101 cattatagtg atccatgcca cattttgtca acgtttattg ctaatcacgt gaatgaatat
    26161 ccagttcact ttcatttgtt gaatactttt gccttcccct gctctccctt aagcgcatta
    26221 ttttacaaaa aacacactaa actcttcctg tctccgataa aagatgatta aatgaaaact
    26281 catttatttt gcataaaaat tcagtgagag cggaaatcca ggctcatcat cagttaatta
    26341 agcagggtgt tattttatga cgacgattct caagcatctc ccggtaggtc aacgtattgg
    26401 tatcgctttt tccggcggtc tggacaccag tgccgcactg ctgtggatgc gacaaaaggg
    26461 agcggttcct tatgcatata ctgcaaacct aggccagcca gacgaagagg attatgatgc
    26521 gatccctcgt cgtgccatgg aatacggcgc ggagaacgca cgtctgatcg actgccgcaa
    26581 acaactggtg gccgaaggta ttgccgctat tcagtgtggc gcatttcata acaccaccgg
    26641 cggcctgacc tatttcaaca cgacgccgct gggccgcgcc gtgactggta ccatgctggt
    26701 tgctgcgatg aaagaagatg gcgtgaatat ctggggtgac ggtagcacct ataaaggaaa
    26761 cgatatcgaa cgtttctatc gttatggtct gctgaccaat gctgaactgc agatttacaa
    26821 accgtggctt gatactgact ttattgatga actgggcggc cgtcatgaga tgtctgaatt
    26881 tatgattgcc tgcggtttcg actacaaaat gtctgtcgaa aaagcctact ccacagactc
    26941 caacatgctt ggtgcaacgc atgaagcgaa ggatctggaa tacctcaact ccagcgtcaa
    27001 aatcgtcaac ccgattatgg gcgtgaaatt ctgggatgag agcgtgaaga tcccggcaga
    27061 agaagtcaca gtacgctttg agcaaggtca tccggttgcg ctgaacggta aaacctttag
    27121 cgacgacgta gaaatgatgc tggaagctaa ccgcatcggc ggtcgtcacg gcctgggcat
    27181 gagcgaccag attgagaacc gtatcatcga agcgaaaagc cgtggtattt acgaagctcc
    27241 ggggatggca ctgctgcaca ttgcgtatga acgtctgttg accggtattc acaacgaaga
    27301 caccattgag cagtatcacg cgcatggtcg tcagttgggc cgtctgctgt accaggggcg
    27361 ttggtttgac tcccaggcgc tgatgctgcg tgactctctg caacgctggg ttgccagcca
    27421 gatcactggt gaagttaccc tggagctgcg ccgtgggaac gattattcaa tcctgaatac
    27481 cgtctcagag aacctgacct acaagccaga gcgtctgacg atggaaaaag gcgactcggt
    27541 gttctcgcca gatgatcgta ttggtcaatt gaccatgcgt aatctggata tcactgatac
    27601 ccgagagaaa ctttttggtt atgccaaaac tggcctgctt tcctcctctg ccacttcagg
    27661 cctgccgcag gtggagaata tggaaaacaa aggccagtaa ttcgacatca accctgccct
    27721 tcaggggtgg ggttaccttg cttatcctct gtaaactcct ttctgagcat cacataatcg
    27781 aaaacatcat ggtttgcatc cacaacccgt gagtctcctt tatagtgtgc aatcacttcc
    27841 tcaagcgttt gcggctgttc cggctttgtt acccccatcc acgcattaat aaggtaatta
    27901 ttgtaagctg tcgaaaagat attgttttcc gttgtgcgat ccacgccatc cccaagaacg
    27961 gggctatacc agataaacat cgggacatga tatgcctgct ggctagcctc cctgcctcca
    28021 tgaaaataga cgttcttctt cgtagggtca cgttccaggc cgtggtcggc aaaatacatg
    28081 accgaggcgc ggcgatcttt taataattca aaaacctgac ctagcaaact atctgtgtaa
    28141 tgaatggagt tgtcatagca ggcatcctga tcgtcctgcg gttgaaacac ggcgctggat
    28201 tgcggatagg cgctacaagc cggttcatgg cttccattta aatgaagaac aatcagtttc
    28261 ttttgctgcg tattttgctg taatgcttgc gataaatgcg gcaacaacaa ttcatcaaat
    28321 cctctgacat aaactgtttc catggcgcgc atggcgatac tggtaactgc tgtaccattc
    28381 tgccgaaaag cggattgcga gcttagccag aaagtctgaa atcctgcttg attagccata
    28441 ttaataatgt tgtcggggta attatgaatg tcatgactca aaacagaatc agcagttaaa
    28501 gaaaggggaa ccgacagcgc agtgtaaggt gcgccgctta ttgcttgatt aaacagtttg
    28561 atctgttttc tttgtgcttc aacttgcggt gtcgtagagc gtgtatatcc atacaaagac
    28621 atattgtcga cacgtacaga ctcccccaca atcaacacgt aggtatcaat acttgtatcc
    28681 ctgactgata attgaaaata cggcaccgtg tttgcaattg agagtaatct ttgatgctct
    28741 ttcgctgcta aagcaaaata gttgagattg aaaaacggcg tataggtagc aaatcacgac
    28801 gctagtatat atggactgaa cgctttttta ttttttgcat ctttataagc aaattgacaa
    28861 gcggaaaata aactgcccga aatgacaatc agcaataata ttcctgtcac tttttttgtc
    28921 ggcaaggaaa catcatattt tattattact gccaaaaaaa gaagggataa aaaagcaaaa
    28981 gcacatagat aagggatata catccccagc attttcacaa cttcatccgg atcactctgc
    29041 agcacactaa tcgcaaatcc atcattgaaa gttgtaccaa aggtacacca tgaatagagg
    29101 ctaatactca tgtctgccgc agtcagtaac acaaatggtg cggcaacaag caggcgaagc
    29161 ggaagggatg atatacgttt aacaaccagg atggtcagga cgaagaacag caatcgctga
    29221 accatatgcg ctccggaaga ggcgactaag ttcgtaatac caaaaacaat aacacaaaga
    29281 tacaacaacc aatgagattt aaaatcttct agcaaatttg ttgaatactg tcatccagaa
    29341 atccaagttc acaaccaata taaacaatta tcaacaatgt tcaattttat atcagacatg
    29401 tttttcaact ctgaaccata tcagaccgaa acactgttag ttttttcttt ttaaatgatg
    29461 cgaccattgc gttctcagta attaaataac attatcaatg cgtctctaaa cattacgcag
    29521 aacgtagcca tcagtagtta tactgacagg agcaggatgg tttatcgaga agtaaacata
    29581 tccgtattcg taccggaact ggagttgaca caataaagtg ccaattatgt cagcagtagg
    29641 gaaaagttta taactaaggc gtaccggcac catcgtttca aggtaccagc tacgagtaaa
    29701 gcaactggac gagatacaga tacctgacaa ccattcatca gaccaggacc aaaacgaaaa
    29761 gagacgcttt tcagcgtctc ttttctggaa tttggtaccg aggacgggac ttgaacccgt
    29821 aagccctatt gggcactacc acctcaaggt agcgtgtcta ccaattccac cacctcggca
    29881 cggatactac tttttagttc gggatatcgc tggtcggctt agccggagca gctggctgag
    29941 tttgttcggt tttcgccggt gcactcagat tttcccattc gctacctcta ttggttttgt
    30001 tgctattgat gttacccagc accagactga tgatgaagaa taacgttgcc agcagcgccg
    30061 tcatgcgggt catgaagtta ccagaaccac ttgaaccaaa cagcgtagcg gaagcgcctg
    30121 ctccgaagga ggctcccata tcagcgcctt taccttgctg cagcatgatc agaccaacaa
    30181 ggccaattgc cacaataagg aaaactacta aaagagcttc gtacataatc aacctgttcc
    30241 ttgcggagtt gccgcgtacc aatgcttcaa ccaataaagc gggagttttt aatctttccc
    30301 actgaagcgg gtgtgaatac taaccaaagc gaatgacctt cgcaagggca attttatcgc
    30361 attgtatcaa ctgcggaaaa aaacagcaaa accgatgtgt tcgccgagaa aaaaggcgac
    30421 aatcgccgcc tttttagcca gttatctaac gctttaaacg gcttttactg catcggcgat
    30481 gcggtgtgca aattcagtca cctgcgcttc gtcttcgcct tccaccatca cgcgaattaa
    30541 cggttcggtg ccggatttac gcagcaacac gcgtccacgg ttgcccagcg cagcttcaac
    30601 ctctgcggtc acggctttaa ctgactcatg ctcaagcgga tcgccgctac ctgcggtgta
    30661 acgtacgtta accagaatct gcgggaacat tttcatgccg ctgcaaaggt cgtgcaggct
    30721 catatggtta cgtgccatcg ccgccagcac ctgcaagcca gcaacgatgc cgtcaccggt
    30781 agtggtttta tccagcagga tcacatgacc ggaattctct gcaccgatac gccagccttt
    30841 ctcctgcatt ttttccagta cgtagcggtc acccactttc gcgcgcgcaa atggaattcc
    30901 taactgtttc agcgccagtt caagccccat gttgctcatc aatgtaccca cagcgccacc
    30961 acgcagctgg ccctgacgaa gaccttcacg cgcgatgata tacatgatct gatcgccatc
    31021 gactttattg ccttcatggt caaccataat cacgcgatcg ccgtcgccgt cgaaggcaat
    31081 accgagatcc gctttttcag ccagcacacg agcctggagc gcgcgaacgt cggtagcccc
    31141 cacttcggca ttgatgttta caccgtttgg ctcacaaccg atagcgataa cgttcgcccc
    31201 cagttcgcgc agcacgttcg gcgcgatgtg ataagtcgca ccgtttgcac aatccaccac
    31261 aatcttcagt tcactgaggc taagttcgtt cgggaacgtg gctttgcaaa actcgatata
    31321 gcgacccgcg gcatcaacga tacggctggc tttacccagt tctgccgaat caacgcagct
    31381 gatctccttt tccatttccg cttcgatggc ctcttctacc gcatccggca gtttagtgcc
    31441 gtcgatagag aagaatttaa tcccattatc gtagaacggg ttatgcgatg cagatatcac
    31501 aattccggcc tctgcgcgga aggtacgcgt cagataagcc acggccggtg ttggcatcgg
    31561 gccagtgaag agtgcggaaa ggcccgctgc cgccagaccc gcttccagtg ctgactccag
    31621 catatagcca gaaatacgcg tgtctttacc aataataatc ttacgggagc cgtggcgcgc
    31681 cagcacttta cccgcggccc aacccagctt aagcacaaaa tcaggtgtga tcggcgcatc
    31741 ccctacacga ccacgaatcc catcggtacc gaaatattta cgattactca tagcgtttgt
    31801 tttcctttgc agacagagtg gcttccacca cccgcatcgc ttctacggtt tctttgacgt
    31861 catgaacacg aatgatgtgc gcgccttgca ttgcggcaat gaccgcacag gccagactac
    31921 cgctcaggcg ctcggacggc cccacgttca gcagctgccc aatcatcgat tttcgtgaca
    31981 tacccaccaa cagcggcagg ttgaaatggt gaaattcagc caggcgcgcc agtaatgaat
    32041 agttatggga gagattttta ccgaaaccga atccggggtc gagcaacaat ttctcttttg
    32101 cgatacccgc ctgctcgcaa cgtgctattt gctcaataaa gtagcgattc acttctgcaa
    32161 agacatcgtc atacttcgga gcttcctgca tggtttttgg atttccctgc atatgcatca
    32221 gacaaaccgg taaaccggtt tctgcagccg cctccagagc gccaggttcg gaaagggagc
    32281 ggatatcatt aataatgtga gcgccaactt tcgctgactc acggatgact tctggtttgg
    32341 atgtatcgac cgagatccag acttcgaagc gttgagcaat tgcctcaacc acaggaataa
    32401 cacgttgcaa ctcttcttca acgctaactt ccgccgcccc tgggcgcgtg gactcgccac
    32461 caacgtcaat gatcgtcgcg ccagcattga tcatcagatt cgcatgtttc accgcatcta
    32521 tcagcgagtt atgcgtgcca ccatccgaaa aggaatcagg cgtgacgttg aggatcccca
    32581 ttacgtgagg atggctaagg tccagtgaag taccctgggc aaagagtttc atgatgttat
    32641 ccctggtatg aattgataag aaaaaacccc ggagcacgcc ccggggtttt cggtacaaat
    32701 acagtcatct gatgcgggaa cttacttgtc gcctaactgc tctgacatgg tgttacccgg
    32761 gttcggcgta cgcggttcat caaccggacg aggagccttt ggactaccat tgtcgccaga
    32821 attgttagaa gcgcctggtt cttcccagcc cgctggcgga cgtacatcgc gacgtgccat
    32881 caggtcatca atctgcggtg cgtcgatagt ctcatatttc atgagagcat ctttcatcgc
    32941 atgcagaata tccatattgt cggtcagaag ctgacgcgca cgattatagt tacgctcaat
    33001 cagtgctttc acttcctggt cgatgatacg tgcagtttca tcggacatat gtttcgcttt
    33061 cgctacgcta cggccgagga atacttcacc ttcttcttcc gcgtacagca gtggacccaa
    33121 tttctcagag aagccccact gagtcaccat gttacgtgcc aggttggtcg caactttaat
    33181 atcgttggac gcaccggtag aaacatgttc cggcccgtag atgatctctt ctgccagacg
    33241 accaccgtac agcgtagaaa tctggctttc cagtttctga cggctggcgc tgattgcgtc
    33301 gccctcaggc aagaagaaag tcacacccag cgcacgaccg cgtgggataa tcgtcacttt
    33361 atgcaccgga tcgtgttccg gcaccaggcg accgataatc gcatgacccg cttcgtggta
    33421 agccgttgat tctttctgcg cttccgtcat caccatggag cgacgttccg cacccatcat
    33481 gattttgtct ttcgctttct cgaactcaac catcgacaca acgcgtttgt tgccacgagc
    33541 agcgaacagt gccgcttcgt tcaccaggtt cgccaggtca gcaccggaga aaccaggagt
    33601 accacgggca atgattgccg cgtcgatatc gggtgccaat ggtacgcgac gcatgtgaac
    33661 tttcaggatc tgctcacgac cgcgaacatc tggcaagccg accacaacct ggcggtcgaa
    33721 acggccagga cgcagcaggg ccgggtcgag aacgtccgga cggttagtcg cggcgataac
    33781 gatgatacct tcgttacctt cgaagccatc catctcaacc agcatctggt tcagagtctg
    33841 ttcacgttca tcgtgaccac cgcccagacc agcgccacgc tggcggccta cggcgtcgat
    33901 ttcatcgata aagatgatgc acggtgccgc tttcttcgcc tgttcgaaca tgtcacgaac
    33961 acgggatgca cccacaccga cgaacatttc tacgaagtca gaaccggaga tagtaaagaa
    34021 cggaactttc gcttcgcctg caatcgcttt cgccagcaac gttttaccgg tacccggagg
    34081 accgaccatc aggacgcctt tcgggatctt accgccgagt ttctggaagc ggctcggctc
    34141 gcgcagatac tctaccagtt cagcaacttc ttcttttgct tcgtcgcagc ccgcaacgtc
    34201 agcaaaggtc gttttgatct gatcttccgt cagcatgcgc gctttgctct taccaaacga
    34261 catggcacct ttgccaccgc cgccctgcat ttgacgcatg aagaagatcc agacaccaat
    34321 cagcaacagc atcgggaacc aggagatgaa gatagaagcc agcaggcttg gttcttcagg
    34381 cggttcaccg acaaccttga cgttcttggt caacaggtta tccagtaatt tcggatcctg
    34441 aaccggaatg taagtggtat aacggttact atctttcttg gtaacgttga tttcacgtcc
    34501 gttgatacgc gcttcacgaa cctggtcgtt attgacctct tgtaggaagg tagagtaatc
    34561 caccttacgg ccattagact cgctgggccc aaagctctgg aatactgaca tcagcacaac
    34621 ggcaatgacc agccagagta ttaggttttt cgccatgtca ctcaagggat taacctctta
    34681 ttacaactgt gttaaaaaca gcgtcaggat actctatatc cagcatcttt caaactttcg
    34741 tctgaaatct cccggttagg gtttacgccc ggtcgctaca atatacactt ctcgcgaacg
    34801 tgcacgagaa gagtccggct tacgaacttt gaccttcgta aacagggagc gaatttccct
    34861 tagatactca tcgaaacctt cgccctggaa caccttcact acaaaactgc cacctggcgc
    34921 taatacatca cgacacattt ctagcgccag ttccaccaga tacatggcac gggggatatc
    34981 caccgccggt gttccgctca tgtttggtgc catatcggac atgacaacct ggactttgct
    35041 gtcgccaacg cgctccagca gtgctttcat caccagttca tcacgaaaat cgccctgaag
    35101 aaagtccaca ccaacgatag gatccatagg taaaagatcg caagcgatga tgcggccttt
    35161 gccgccaatt tgggtgacca catattgtga ccaaccaccc ggagcagcac caaggtcgac
    35221 aaccgtcatt cccggtttaa agagtttgtc actttgctgt atttcatcaa gtttaaacca
    35281 ggcacgggaa cgtaatccct ttttctgtgc ctgttgaaca tatttatcgc taaagtgttc
    35341 ctgaagccag cggctggagc tggcagaacg cttcttacct gtcatttaac tttcccatgg
    35401 ggataactca tcgtaaccaa ttgcgtaaat ttttacgcgc ctatttggtg atatatggga
    35461 gatggcggta gaatgacccg ttttcaatcc caacgtaagc aaaaaatacg atgaatctga
    35521 gtactaaaca aaaacagcac ctgaaaggtc tggcacatcc gctcaagcca gttgttctgc
    35581 ttggcagtaa tggtttgacc gaaggggtgc tggccgagat tgaacaagcg ttagagcacc
    35641 atgaactcat caaggtgaaa atcgccaccg aagatcgcga aacaaaaacc ttgatcgtgg
    35701 aagctatcgt gcgcgaaacc ggcgcctgta atgtacaggt catcggtaaa acgctggtgc
    35761 tttatcgccc aactaaagaa cgtaaaatct cgctgccacg ctaagattat cctaaagtta
    35821 cacacatttg ctgtgtaaaa cgaggggttt tccgcaggca ggagagcaaa atgccacgct
    35881 ctgttcgttg ataaaaggcc gcagagcggc ctttttcctt tctttacaat acatcaacat
    35941 cttgagtatt gggtaattct tacaggtatt ccaccttaat tacttcaaat tctacttcgc
    36001 cgcccggcgt tttgatgacc acaacatcat cttcttcttt gccgatcagg ccacgagcaa
    36061 taggcgagtt tacagaaatc aggttttgtt taaagtcagc ttcgtcatcg ccaacgatgc
    36121 gataagtctg ttcttcgtca gaatccagat tcagcaccgt tacggtagca ccaaaaataa
    36181 cgcgcccatt gttgggcatt ttggtgacat caatcacctg cgcgtttgac agcttggctt
    36241 cgatgtcttt gatacggcct tcgcagaagc cctgctgttc acgagctgcg tggtattcgg
    36301 cgttttcttt caggtcgcca tgctcacgcg cttccgcgat agcagcaatg atttcaggac
    36361 ggcgcacaga tttcagaaaa tccagctctt cgcgtaattt ttcagcgccg cgtaaggtca
    36421 tcggaatagc ttgcatttgt tatacctctt gaatattcct gatggggcaa gtcttcaccc
    36481 accccggctg ttcggcctgc ccggcataac tcctcaccgg gaccagaagc aaaaaaatac
    36541 cgacccgggt acaagtccca ggtcagctac aattcacatt ttgatggtca ttttaccctg
    36601 aagttcccga agggtcatcg tttactttat agggcgttgc gccgtagtat gacggctcga
    36661 ttccaggttg ttagcgcgag attatgcgat tttccagatt tatcatcgga ttgaccagct
    36721 gtatagcgtt cagtgttcag gccgcgaatg ttgatgagta cgttactcaa ctccccgcag
    36781 gtgccaacct tgccctgatg gtgcaaaaag tcggtgcgtc ggcccccgct attgattacc
    36841 acagtcagca gatggcgctg cccgccagta cccagaaagt gattactgcg ctggcggcgt
    36901 tgattcaact cggccccgat tttcgtttca ccacgacgct tgaaaccaaa ggcaatgtgg
    36961 aaaacggcat acttaagggt gacttagtgg cgcgatttgg tgccgatccg acgttaaaac
    37021 gtcaggatat tcgcaatatg gtcgcgactt tgaaaaaatc tggcgtcaac caaatcgatg
    37081 gcaatgtgtt gatagatacc tccattttcg ccagccacga taaagccccc ggctggccat
    37141 ggaatgacat gacacaatgc tttagcgctc cgcctgctgc cgccatagtt gaccgcaact
    37201 gtttctccgt ctcgctctac agtgccccaa agcctggtga tatggctttt atacgcgtgg
    37261 catcttatta ccccgttacg atgttcagcc aggtacgcac cctcccccgt ggttctgccg
    37321 aagcgcaata ctgcgaactg gatgtggtgc caggcgacct gaaccgcttt acgctgacgg
    37381 gatgcctgcc acaacgttct gagccgctcc cgttggcttt tggcgtgcag gatggagcca
    37441 gctatgccgg tgcaattctg aaagatgagt taaaacaggc gggtatcacc tggagcggaa
    37501 cactgctgcg ccagactcag gttaacgaac ctggaacggt agttgccagt aaacagtcgg
    37561 ccccgctgca cgatctgctt aagattatgc tgaaaaagtc ggacaacatg attgccgata
    37621 cggttttccg catgatcggc catgcgcgct tcaatgtgcc tggaacatgg cgggctgggt
    37681 cggacgccgt gcgtcagatc ctgcgccagc aagccggtgt cgatattggt aacaccatta
    37741 ttgccgatgg ttcagggctt tcgcggcata acctgattgc ccccgccacc atgatgcagg
    37801 tgctgcaata cattgcccaa cacgacaatg aacttaactt tatctccatg ctgccgcttg
    37861 cgggctatga cggctctttg cagtaccgtg caggtctgca tcaggcgggc gtggatggca
    37921 aagtctcagc gaaaaccggt tcgttgcagg gggtatataa cctggcggga ttcattacca
    37981 cagcgagcgg gcaacgaatg gcatttgtac aatatctttc tggctatgca gtaaaacccg
    38041 cggatcagcg taatcgccgt attccgttag tgcgttttga aagccgtttg tacaaagata
    38101 tttatcagaa caattagtca aaaagaaacc ccggcgcatg gccggggttt cagattattg
    38161 acaaagtgcg ctttgtttat gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc
    38221 gtgcaaattc aatatattgc aatcatcttg taggcctgat aagtgtagcg catcaggctt
    38281 atttggcatt ggtcattagt gattaacgct tgtaaatgaa ctcaacgcct tcttcgtcgt
    38341 cttcgtccca gtcgtcatcc cagtcttcat catcctcttc agcaatctct tcaagctgct
    38401 gacggtgata atcatcccac atgaattcga ctttctctgg ctgtttcgct tcttcagcct
    38461 gcacgaccgg gttttcaatg ataaaggtca tcacatccca gcagagatct ttcacgccca
    38521 gtccgctcgc cgcagagatc agataatatt tatcctccca gcccagcgct tcagcgatcg
    38581 ctttcgcttt ctcttcggct tctgccttat ccagcagatc gatcttgttg aagactaacc
    38641 aacgcggttt cgccgccaga tcctggctgt atttttccag ctcgctgata ataatacgcg
    38701 cgttttcaac cggatcggtg ccgtcaatcg gatcgatatc gatgaggtgc aacaggacgc
    38761 ggcaacgttc caggtgcttc aggaagcgaa tgcccagacc tgcgccttcc gcagcgcctt
    38821 caatcagtcc tggaatatcg gcaacaacga agctcttttc gttgtccatt cgtaccacac
    38881 ccagacttgg caccagagtg gtaaacggat aatccgccac tttcggttta gccgccgata
    38941 ccgcacgaat aaaggtcgat ttacccgcgt ttggcatccc caacataccg acgtcagcca
    39001 gcagcatcag ctccagcagc agctcacgct tatcgcccgg cgtaccgtta gttttctgcc
    39061 gtggtgtacg gttaactgac gatttgaaac gggtattgcc cagaccgtgc cagccgccct
    39121 tagcaaccag cagacgctga ccgtgtttag tcatatcacc catggtttca ccagtaccct
    39181 ggtcgattac acgcgtacct accggcactt taatcgtcac gtctttaccg cgcttaccgg
    39241 tacaatcgcg gcttgcgccg ttctgaccgc gctctgcacg gaaagatttt tcaaaacgat
    39301 aatcgataag cgtgttcagg ttctcgtcgg cttccatcca tacgtcacca ccatcaccgc
    39361 cgtcgccgcc atccgggccg cctttcggaa tatacttttc gcggcggaag ctcacgcaac
    39421 cattaccgcc atcacctgca acgaccagaa tcgatgcttc atcaacaaac ttcattttat
    39481 tctccgtaaa tcattcgcct gcgcggggtt gcgataccac cgttgtatgc ttacgtaatc
    39541 cgccccaaat acgatgacca atggcggaat acatcgcgcc cgcaaccacg acaaacgcac
    39601 cgagataacc taaaaggttt aacatcggtc tggcgaagaa atcgggccag gccagtgata
    39661 aaagatctga aaaaaacagc gtaaacagtg gggtgagcgt gatgatcgcg ctcacctgcg
    39721 ctgcctgcca gcgagccatc gcttccgcca aggcgccata tcctaccaag gtattcagtc
    39781 cgcaaaaaat taaacatgcg agctgccagt ggctaagctg cgctatcact ccaggctttg
    39841 ccagagggaa gagcgcgatt gtacataaag tgtacagtaa aaacaggatc tgcggtgagg
    39901 ccagccgacg caataaaacc ttttgcgcca ctccataact cacccaaacc gtcgccgcac
    39961 cgaccccaaa gataactccc caggtgtaat cggtgagctt tgtaaatatc tcgaccagac
    40021 tggtgttaaa aaacatcacc agaccgctca ggagcatcaa tgcccctaca acctgagtgc
    40081 tgcgcatttt ctctttcagg ataaatacgc tggcaaccat catgccaact ggcgagagtt
    40141 gcccaatcac ctgcgaagcg gtcggactca ggtattgcaa ggatgagctg aacaggatga
    40201 agttcccaaa cagcccggcg gtcgccactg ccaacaaaat caaccagcgt ggcttacgaa
    40261 acacgcgtaa tggcgacaac ctcttcttca ccgcaagaat ggcaccaagg ccaatactcg
    40321 ccatcaagaa acggtaaaac acgattgtcg gaggttccat cacctccagc acctgcttca
    40381 ttgcgattgg caacgccccc cagcaaattg ctgtggtaag cgccaaaaga atgccaatgc
    40441 ctgcctgctg cttcataccg tattccctgc aaagcgcatt tgcggattaa cgtcgcacca
    40501 tgcgcatttt taccggttat cgaatgtaaa aagccccgca acgtgttgcg gggctttcat
    40561 ccgttaccgg gacgcgaaaa acttattcag cttcgatgct gataaattta cggtttttcg
    40621 ggcctttaac ttcgaatttc actttaccgt ctgctttggc aaacagagtg tggtcacgac
    40681 cgcaacctac gttagcgcca gcgtggaatt tggtaccacg ttgacgaacg atgatgctac
    40741 ccgccagaac ggattcgcca ccgaaacgct taacgcccag gcgtttagct tctgaatcgc
    40801 gaccgttacg tgtggagccg ccagcctttt tatgtgccat ttgaaatctc tcctcaggtc
    40861 ttaggcgctg atgccagtaa ttttcacatc agtgaaccac tgacgatggc cctgctgctt
    40921 acgatagtgt ttacgacgac gaaacttaac gattttaact ttctcgccac gaccgtgagc
    40981 aacaacttca gctttgatta cgccgccatc aacgaaagga acgccgattt tgacttcttc
    41041 accgtttgcg atcatcagca cttcagcgaa ctcaacagtt tcgccagttg cgatgtccag
    41101 cttttccagg cgaacggtct gaccttcgct tactcggtgt tgtttaccac cactttggaa
    41161 aaccgcgtac atataaaact ccgcttccgc gcacaccttt tcaatgattc agagtgcgct
    41221 ataaatattc acaatagggc gcgaatatta cgcaaaacgc acgcctttga caagtgctac
    41281 agtcaataca cgaagaaaaa aaacacaact tgtacggtaa cgtttatctg tgccattttt
    41341 tcagtacaat taccctatat tcctaaccat aaaccctaag ttgcctttgt tcacagtaag
    41401 gtaatcgggg cgaaaagccc ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc
    41461 gcgcaagata tggcgggtgt taatgcggca atccttgagc agcttaattc cgacgtccaa
    41521 ctgatcaatc agttaggcta ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg
    41581 attgctgtac tggctgcacg agctgttggc tatgagggaa atgcgcatgt caccattgct
    41641 gccctgatcg agtttatcca cacggcgact ctgctacacg acgacgttgt ggatgaatca
    41701 gatatgcgca ggggtaaagc taccgccaac gccgcatttg gcaatgccgc cagcgtgctg
    41761 gtaggcgatt ttatttatac ccgcgcgttc cagatgatga ccagcctcgg ttcactcaaa
    41821 gtgctggaag tcatgtcaga agctgtaaac gtcatcgcag aaggtgaagt tctgcaactg
    41881 atgaacgtta acgatccgga catcactgaa gaaaactaca tgcgcgttat ctatagcaaa
    41941 accgcgcgtc tgtttgaggc tgccgcgcag tgttccggga ttctggctgg ctgtacgccg
    42001 gaggaggaga aaggcctgca ggattatggg cgctatctcg gcactgcttt ccagttgatc
    42061 gacgatttac tcgattacaa tgccgatggc gaacagttag gtaaaaacgt cggcgacgat
    42121 ctgaacgaag gtaaaccgac gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa
    42181 caggcacaga tgatccgtac cgccatcgaa caaggtaacg gtcgccatct tctggaaccg
    42241 gttctggaag caatgaacgc ttgtggatct cttgaatgga cgcgtcagcg cgccgaggaa
    42301 gaagcagaca aagccatcgc agcgttacag gtgctcccgg acaccccttg gcgagaagca
    42361 ctcatcggcc tcgcgcacat cgctgttcaa cgcgatcgtt aatcccctcc cttcatcccg
    42421 cgcagtgcgc gggatgattc cagtaaattc cataaaaaca ctaattcagc gctataacac
    42481 ctgaaaaaaa gggcgatgaa tctttatata ttctgaatat tcacactctt tacaggaact
    42541 ttttagcgca ataggccatc aggagtatag tgatgcccga cagaagaagt gttctgaatg
    42601 aaagcgacta acttaaggag tgaggaaaga tggaaagtaa tttcattgac tggcatcccg
    42661 ctgacatcat tgcaggtttg cgcaagaaag gaacgtcaat ggcggcggaa tctcgcagaa
    42721 atggtttgag ttcctcaacg ctggcaaacg cattatcgcg cccatggccg aaaggagaga
    42781 tgattattgc gaaagccctg ggaactgacc cctgggttat ctggccatca cgctaccatg
    42841 atccgcggac ccatgagttt atcgacagaa cgcagttgat gcgtagctac actaaaccga
    42901 aaaaatgagt ggtctggcgg tagccccgcg aacggggctg ccagctctca gacgattact
    42961 cgcctttcac acgctcaata tttgcaccta aagcgcgcag tttgtcttca atgcgttcgt
    43021 agccacgatc gatgtgataa atacgatcaa ccaccgtcgt cccttccgca atacagccag
    43081 ccagcaccag gcttgctgat gcacgcagat cggttgccat aacctgtgca ccagaaagtt
    43141 tttcaacacc atgacaaata acggtattgc tttcgatttc ggcgtgcgcg cccatacggc
    43201 tcagctctgg cacatgcata aagcggtttt caaagaccgt ttcggtgatg aacccggtcc
    43261 cttcagccac caggttcaac agcgtgaact gggcctgcat atcggtcggg aatgccggat
    43321 gcggcgcggt acgtacgtta acagccttcg gacgtttgcc atgcatatcc aggctaatcc
    43381 agtcttcgcc gacttcgatg tccgctccag catcacgcag tttcgccagc acggcgtcca
    43441 gagtatctgg ctgcgcgtta cggcagataa ttttgccacg agagatcgcc gccgccacca
    43501 ggaaagtacc ggtttcgata cgatccggca gcacgcgata gacaccgccg cctaaacgtt
    43561 ccacaccttc gatgacgata cgatcggtgc cctgaccgct aattttcgca cccagcgtaa
    43621 tcaggaagtt cgcggtatcg acgatttccg gttcacgcgc tgcgttttca ataatcgtgg
    43681 tgccttccgc cagggttgca gcacacatga tggtcaccgt tgcgccaacg ctgactttat
    43741 ccatcacgat atgtgcgcct ttcagacgac catcgacgga agctttaacg taaccttctt
    43801 ccagtttgat ggtcgcgcct aattgttcga ggccagaaat gtgtagatca accggacgag
    43861 caccgatcgt acaaccgcca ggtagtgaaa cttgcccctg accaaagcgc gctaccagcg
    43921 gccccagcgc ccagatagaa gcacgcatgg ttttaaccag atcgtaaggt gcgcagaata
    43981 cattaacgtc gcgggcatca atatgcacag aaccattacg ttctactttc gcacccagct
    44041 ggcttagcag tttcattgat gtatcgacgt ctttcagttt cgggacgttc tggatctcta
    44101 ccggttcttc cgccagtagt gcggcaaaaa gaataggcag agcagcattt ttagcgccgg
    44161 aaattgtgac ttcgccctgg agcttcgttg gcccctgaac acgaaattta tccatttagt
    44221 ttgttctcag ttaacaattc atatccgcta ccggcgaatc gcccatagct caaaagccgt
    44281 tcagtttgcg atcgcgcgcc cactccgcag gggtatacgc tttgatcgac acagcatgaa
    44341 tgcggttatc cgcaatatat tccatcagcg ggccatagac cgtctgctgt tttttaaccc
    44401 gactcatacc gtcaaacaac tcacccacgg caataacctg aaagtgactg ccatcgccgg
    44461 aaacgtggac ttcctggagg gagagagcgt tcatcaacac gctctgaatt tcattatttt
    44521 ccatgggatc ttcaatcatc agttaataaa ccagcgaaac atcttagagc aaagttgcgc
    44581 tggcataaat aagcaaaaag cctcgctgat aaatcagaca aggctcgact tgcaggcagg
    44641 tttgccggac aggcggttaa cgccatatcc ggcctgaaaa aatttaacga ggcagaacat
    44701 cagcaggcaa attatacaat ttcgcgaggg tatacacttt gtcgtttacc ccctgaagcg
    44761 tcacgttgtt gccctgcttt ttcgccagat cgataagatg gagcagcagt gccagtcccc
    44821 ccgtatccac gcgggacaca cggctaagat cgatgcaggt aatccccttc accgcttcct
    44881 cacgcatttc ccaaagcggt agcagaacgt cctgatccag ctctccggat aacgccagcg
    44941 tgtcacccgt ctgcatccag ctcagtgact cgctcattat tttttctctt ccagagtgat
    45001 tttctgttga gaaatcgatt tcagttgcgc agtcaggccg tcgatacctt tggtacgcag
    45061 cagcgttccc cactcgtttt gtttggtggt gatcatactg acgccttcag caatcatgtc
    45121 gtaagcttgc cagtttcccg tctgggagtt tttacgccac tggaagtcca gacgcaccgg
    45181 cggacggcca ttcgggtcaa taatggtaac gcgaataggc acaatggttt tatcgcccag
    45241 cggctgttct ggcgcaatct gataggtttg accgtgatac atcgccagcg cctgaccgta
    45301 agcctgcttc aggtactcac ggaaagcggc aaagtaggct tcacgttgag caggggtcgc
    45361 actcttgtaa tactggccca gcaccagcgc accggcgtat ttcacctgta cgtatggcag
    45421 cagttcctga tcaacaatgg tacgcagata atccgggttg gcccgaattt gcggttgctc
    45481 attcttcagg cgatcgaacg ttttctgcgc cgcctcgtcc atcagcttat aaggattggt
    45541 ctggtctgcc gcggttgccg cactcagagg tgcaatcacc agcaaagcga ccatcattaa
    45601 acgtttaaac atgcgtcggt tctcctgaaa ttatttcgtt gtacccacag gttcagtggt
    45661 ttcattatta cctggcgcag cagctggcgc atcgccacta ttcttattgt catcgccttt
    45721 actaccgtaa aggaactgac caatgagatc ttccagcacc atcgcagact tggtgtcctg
    45781 aattgtatcg ccatccttca ggatagcagt ccccagttcc gggtcttcaa aaccgacgtt
    45841 taatgccaga tattgttctc ccagcaggcc ggaagtacga atgctcagcg aactggtatc
    45901 aggaatgtgg ttataacgtt gttcaatttc cagcgttacg cgcggcagat aggttttcgg
    45961 gtccagcgta atatccgcca cccggcccac aacaacacca ccaatgctga ccggagagcg
    46021 cgctttcagg ccgccaatgt tatcgaacgt cgcataaagc gtgtaggtcg attcagtacg
    46081 tatggacgtc acgttcgccg ccttcaggca aacaaacagc gccgccagca gtgctgctaa
    46141 taaaaagata cccacccaaa tttcattttt tttcgtttgc atgaactcaa ttcccaaaca
    46201 tcaatgcggt cagcacaaaa tccagcccca gaacagccag agacgagtgg acaacggtgc
    46261 gagtggttgc ccggctaatc ccggcagacg tcgggatggc gtcgtagccg ttaaacaacg
    46321 aaatccacgt caccgtgatg gcgaacacca cgctcttaat cagacagttg accagatcca
    46381 tacgccagtc gacggcattt tgcattgccg accagaagaa cccgctatca atgcctttcc
    46441 aactgacgcc gaccagcgat ccgccccaga tccccacggc gacgaagata accgtcaaca
    46501 gtggtaatga aataacccca gcccagaaac ggggagaaat aacccgacgc agcggatcca
    46561 ccgccatcat ctccatactg gagagttgct ctgtagcgcg catcaggccg atttctgcgg
    46621 ttaacgccga accagcacgc ccggcaaaca acagcgcggc aaccaccggc cccagttcac
    46681 gcagtagcga taacgccacc agcataccca gactggtttc cgcgctataa gtggtcagaa
    46741 ccagataacc ttgcagcccc aacaccattc cgatgaacac gccagaaact acaataatca
    46801 gcatcgacag gacgccgaca ttatagagct ggcgcaccag cagcggcaca tgtttgcgaa
    46861 attccggttt gccgaccagc gcattgaata acattaaccc ggcccgcccg aacgttctca
    46921 gggttttaat ccctttatgt ccgagcgacg ccagcgcatt taacagcatg agtggcttaa
    46981 ctccctggta aaagatcagc gtgataatcg ccggcaggat agcggaacgg aacaggcccg
    47041 tcagctatcc cgtccagaaa ctgacgtacg cgcggatcag gattcgtctg caacgcctgg
    47101 gcactgccat gagcgacaat ttttttgtcc gccaggatcc aggcgtgatc cgcaatactt
    47161 aacacttccg gcacatcgtg agaaaccacc acacaagtca cgcccagcgc gctgttcagc
    47221 tcagaaatca gcttcaccag tacgcccatg gtgatgggat cttgcccaac aaaaggttca
    47281 tcaaacatga tgagatccgg ctccagcgca atcgcacgcg ccagcgctgc acgccgcgcc
    47341 atcccaccgg aaagttcaga aggcattagt ttagccgctc cacgcagccc cacggcctcc
    47401 agcttcatca tcaccgtact atgcaacaat ggcgcgggaa gttgggtatg ttcgcgcagt
    47461 ggataggcga cgttgtcaaa tacgttcata tcagtgaaca acgccccgga ctgaaataac
    47521 atgctcatcc gtttacgcac tgtatacagg cgcgaacgag acatcgccgg aatattctca
    47581 ccatcgaaaa ggatctcacc atgatctggt gcgatttgcc cgccaatcag acggagtagc
    47641 gtcgttttac cgatgcccga tggccccatg atcgccgtga tcttccctcg cggcacggtc
    47701 agggaaatat tatcgaagat gcagcgattg ccacgcgtaa aactgacatc gcgcatatcg
    47761 actaaattcg ccacagactg ctccataatt cacccttcgt cttgcgttga ttttctaagc
    47821 atggcgctca atttaacctt gaacccaaca tatttacaga atattacccg ccgtggttag
    47881 cgaaagctgg catttgtttt actttttagc cgcataaagt caaaattaag aatccgttac
    47941 ggctttctga aaatcttcag cggaccggcg agtatacctg aagaaaggac gttagatgct
    48001 tttagctacg gcactgttaa ttgttggttt acttttggtc gtttacagtg ccgaccgcct
    48061 ggtttttgcc gcgtctattc tttgccgaac ctttggcatc ccgccgctga tcatcggcat
    48121 gacggtggtc agtattggta catcgttacc agaaatcatc gtctcgcttg ccgcgtctct
    48181 gcacgaacaa cgcgatttag ccgttggtac agccctcggc tcaaacatta tcaatatatt
    48241 gctgctcctc ggtctggctg cgctggttcg tccttttacc gtccattccg atgttctgcg
    48301 ccgtgaatta cccttaatgt tgttggtcag cgtggtggcc ggttccgtac tctatgacgg
    48361 acaacttagt cgaagcgatg gtatctttct cctctttctg gctgtgctat ggctgctgtt
    48421 cattgttaaa cttgcacgtc aggggactga cagcctgacc agagagcagc ttgcagagct
    48481 gccgcgtgac ggcggattgc ccgtcgcgtt tttatggctc ggcattgcgc ttatcatcat
    48541 gccagtggcc acgcggatgg tggttgataa cgccacggtg ctggcgaatt actttgccat
    48601 cagcgagttg acgatgggtc tgacggcaat tgctatcgga accagtctgc cggaactggc
    48661 aaccgcaata gcgggggttc gcaaaggtga aaacgacatt gctgtcggaa atatcattgg
    48721 cgcaaacatt tttaatattg tcatcgtgtt gggtttaccc gcgctgataa cgccaggaga
    48781 gattgatcca ctggcgtaca gtcgtgacta cagcgtgatg ttgctggtga gcattatttt
    48841 tgcgttgctg tgctggcggc gctccccgca accgggccgt ggtgtagggg tattattaac
    48901 tggcggattt atcatatggc tggcgatgtt gtactggtta tcgccaatac tcgttgaata
    48961 actggaaacg cattatgtcg cacgtagagt tacaaccggg ttttgacttt cagcaagcag
    49021 gtaaagaagt cctggcgatt gaacgtgaat gcctggcgga gcttgatcaa tacatcaatc
    49081 agaatttcac gcttgcctgt gaaaagatgt tctggtgtaa agggaaagtt gtcgtcatgg
    49141 ggatggggaa atcggggcac atcgggcgca aaatggccgc aacgtttgcc agcaccggta
    49201 caccttcatt tttcgtccat cctagtgaag ccgcgcatgg tgatttaggc atggtcaccc
    49261 cacaggatgt ggtgattgct atctctaact caggtgaatc cagcgaaatc acggccttaa
    49321 ttccagtgct taagcgtctt cacgtaccgt taatctgcat caccggtcgc ccggagagca
    49381 gcatggcgcg cgccgcagat gtgcatctgt gtgttaaagt agcgaaagaa gcctgtccgt
    49441 tagggctggc accgaccagc agcaccaccg ccacgctggt tatgggcgat gccctcgctg
    49501 tcgcgctgtt aaaagcacgc ggctttactg ctgaagattt tgcgctctca cacccaggcg
    49561 gcgcactggg tcgtaaactt ctgctgcgcg taaacgatat tatgcatacg ggcgatgaga
    49621 tcccgcatgt taagaaaacg gccagtctgc gtgacgcatt gctggaagtt acccgcaaaa
    49681 atcttggtat gactgtcatt tgcgatgaca atatgatgat tgaaggcatc tttaccgacg
    49741 gtgatttacg ccgtgtcttc gatatgggcg tggatgttcg tcagttaagt attgccgatg
    49801 tgatgacgcc ggggggaata cgtgtgcgcc ctggcattct ggccgttgag gcactgaact
    49861 taatgcagtc ccgccatatc acctccgtga tggttgccga tggcgaccat ttactcggtg
    49921 tgttacatat gcatgattta ctgcgtgcag gcgtagtgta aagattcaag gataaacaac
    49981 aatgagcaaa gcaggtgcgt cgcttgcgac ctgttacggc cctgtcagcg ccgacgttat
    50041 ggcaaaagca gagaacattc gtctgctgat cctcgacgtt gatggcgtac tgtcagatgg
    50101 cctgatttat atgggcaata acggcgaaga gctgaaagcg ttcaatgttc gtgacggtta
    50161 tggcattcgt tgtgcgctca cctctgatat tgaagtcgct atcattaccg ggcgaaaggc
    50221 taaactggta gaagatcgtt gtgccacatt ggggatcact cacttgtatc aggggcagtc
    50281 aaacaaactg atcgccttta gcgatttgct ggaaaaattg gcgattgccc cggaaaatgt
    50341 ggcttatgtt ggcgatgatc tcatcgactg cccggtaatg gaaaaagtgg gtttaagcgt
    50401 cgccgtggcc gatgcgcatc cactgttgat cccgcgcgcc gattacgtga cgcgcattgc
    50461 tggcggtcgt ggcgcagtgc gcgaagtttg cgacttatta ctcctggcgc agggcaaact
    50521 ggatgaagcc aaagggcaat cgatatgagt aaagccagac gttgggttat cattgtgcta
    50581 tcactggcgg ttctggtgat gatcggcatt aatatggccg aaaaagacga taccgcccag
    50641 gtggtcgtca acaacaatga tcccacctat aaaagcgagc atacggacac gctcgtctat
    50701 aacccagaag gggcactaag ctatcgattg attgctcaac acgttgaata ttattccgat
    50761 caggccgttt cgtggtttac gcagccggta cttaccacgt ttgataagga taaaatcccg
    50821 acatggtccg taaaagcaga taaagccaag ctgaccaatg accggatgct ctatttatat
    50881 ggacacgttg aagtcaacgc acttgtgcca gactctcaac ttcgcagaat cacgacggat
    50941 aacgcgcaga tcaatctggt gacgcaggat gttacctctg aagacctcgt cacgttatac
    51001 ggaacaacat ttaactccag cggtctgaaa atgcgcggca acttacgcag caagaacgcc
    51061 gagctgattg aaaaggttag aacatcctat gaaattcaaa acaaacaaac tcagccttaa
    51121 tcttgtgctt gccagctcac ttctggccgc cagcattccg gcatttgccg taaccggaga
    51181 cactgatcag ccgatccaca ttgaatcgga ccagcaatct cttgatatgc aaggcaacgt
    51241 ggttaccttt accggtaatg tcatcgtcac ccagggcacc atcaaaatta atgccgacaa
    51301 agtggtcgtt acccgtccgg gcggcgaaca aggtaaagaa gtgattgacg gatacggtaa
    51361 accggcaacg ttctaccaga tgcaggacaa cggtaaaccc gttgaaggtc acgcttccca
    51421 gatgcactac gaactggcaa aagattttgt cgttctgacg ggtaatgctt atctgcagca
    51481 ggtcgatagc aacattaagg gcgataagat cacttacctg gtgaaagagc agaaaatgca
    51541 ggctttcagc gacaaaggca agcgcgtaac caccgttctg gtgccgtcgc agctgcagga
    51601 caaaaacaac aaaggccaga ccccggcaca gaagaagggt aattaattcg ttatggcaac
    51661 attaactgca aagaaccttg caaaagccta taaaggccgt cgcgtggtag aagacgtcag
    51721 cctgaccgtc aactccgggg aaattgtcgg tctgctgggg ccaaacggtg ccggtaagac
    51781 caccactttc tacatggttg taggcattgt gccgcgcgat gcgggcaaca tcattattga
    51841 tgatgacgat atcagtctgc tgcctctgca tgcacgcgcg cgccgcggta tcggctatct
    51901 gccacaggaa gcctccattt tccgtcgcct cagcgtttac gataacctga tggcggtact
    51961 gcaaattcgt gacgacttgt ctgctgaaca acgtgaagac cgcgcgaacg agctgatgga
    52021 agagtttcac attgagcacc tgcgtgacag catggggcag tcactctccg ggggtgaacg
    52081 tcgccgtgta gaaattgccc gcgcactggc tgcgaatccg aaatttattc tgctcgacga
    52141 accgtttgcc ggggttgacc cgatctcggt tatcgatatt aaacgcatca ttgaacacct
    52201 gcgcgacagc ggcctgggcg tgctgatcac tgaccacaac gtgcgtgaaa cactggcggt
    52261 ttgtgaacgc gcttatatcg tcagtcaggg gcatttgatc gcccacggca cgcctacaga
    52321 aatcttacaa gacgaacacg ttaagcgtgt ataccttggg gaagacttca gactctgata
    52381 gggtagaagt ttgcgacgtt ttagcaggag agtacgattc tgaacatgaa gcaaggtttg
    52441 caactcaggc ttagccaaca actggcgatg acgccacagc tccaacaggc aattcgtctg
    52501 ttgcagttgt cgacgctgga acttcagcag gagctacagc aggcgctgga gagtaatccg
    52561 ctgcttgagc aaatcgacac tcatgaagaa atcgacaccc gcgaaacgca agacagtgaa
    52621 acgctggaca ccgccgacgc gctcgaacaa aaagagatgc cggaagagct gccgctcgat
    52681 gccagttggg acaccattta caccgctggt acaccatccg gcaccagcgg tgactacatt
    52741 gacgacgagc tgccggtcta ccagggcgaa acgacgcaga ccttgcagga ttacctgatg
    52801 tggcaggtcg agctgacacc gttttccgac actgaccgcg ctattgctac ctctatcgtc
    52861 gatgccgttg atgaaaccgg ttatctgact gtcccgctgg aagatattct cgaaagtatg
    52921 ggcgatgaag agatcgacat cgacgaggtt gaagccgtcc ttaagcggat ccaacggttt
    52981 gatccggtcg gtgtagcggc aaaagatctg cgtgactgcc tgctaatcca actctcccaa
    53041 ttcgataaga ccacgccgtg gctggaagag gccagattga tcattagcga tcatctcgat
    53101 ctgttagcca atcacgactt ccgcacttta atgcgcgtca cgcgtctgaa agaagatgtg
    53161 ctgaaagaag ccgtcaatct gatccagtcg ctcgatccgc gccccgggca gtcgatccag
    53221 actggcgaac ctgagtatgt cattccagat gtgctggtgc gtaagcataa cggtcactgg
    53281 acggtagaac tcaacagtga cagcattccg cgtctgcaaa tcaaccagca ctacgcctcg
    53341 atgtgcaata acgcgcgcaa cgatggtgac agccagttta tccgcagcaa tctgcaggat
    53401 gccaaatggt tgatcaagag tctggaaagc cgtaacgata cgctactgcg cgtgagtcgc
    53461 tgtatcgttg aacagcagca agccttcttt gagcaaggtg aagaatatat gaaaccgatg
    53521 gtactggccg atatcgccca ggctgtcgaa atgcatgaat cgacgatatc tcgcgtgacc
    53581 acgcaaaaat acctgcatag tccacgaggc atttttgaac tgaagtattt cttttccagt
    53641 cacgtcaata ccgagggcgg cggcgaagct tcctccactg cgattcgtgc actggtgaag
    53701 aaattaatcg cggcggaaaa cccagcgaaa ccgttgagcg acagcaagtt aacctctttg
    53761 ctgtcggaac aaggtatcat ggtggcacgc cgcactgttg cgaagtaccg agagtcttta
    53821 tccattccgc cgtcaaacca gcgtaaacag ctcgtttgac ccaaccgata aggaagacac
    53881 tatgcagctc aacattaccg gaaataacgt cgagatcacc gaggcactgc gcgaatttgt
    53941 tacagctaaa tttgccaaac ttgagcaata ttttgaccga atcaaccagg tctatgttgt
    54001 tctgaaagtg gagaaagtca cccacacctc agatgcaaca ctgcatgtaa acggtggtga
    54061 aattcatgcc agcgcggaag gtcaggacat gtacgctgcc attgatggct taattgataa
    54121 gctggcacgc cagttgacca aacataaaga taaactgaaa caacactaat tgtccgggca
    54181 attagcatgt gcatggcggc ctgttgtgcg gcacaacggg ccatttgtac ggttaatgct
    54241 ccgagcctgt tccactgttt gagtgggcag gttcttaggt gaaattatga caaataatga
    54301 tacaactcta cagcttagca gtgttcttaa cagggaatgt acgcgaagcc gcgtccactg
    54361 tcagagcaaa aaacgcgccc tggaaatcat cagcgagctg gcggcgaaac aacttagcct
    54421 accgcctcag gtggtttttg aagctatcct gacgcgggaa aaaatgggca gtaccggtat
    54481 cggcaatggt attgccattc cgcatggcaa acttgaagaa gatactctgc gtgccgttgg
    54541 cgttttcgtt cagctcgaaa cgcctatagc tttcgatgcc atcgacaacc aaccggtgga
    54601 cttacttttt gccctgctgg tgccggcaga ccaaactaaa acgcacctgc atactctgtc
    54661 gctggtggcg aaacgtctgg cggacaaaac catctgccgc cgtttacgcg cagcccagag
    54721 cgatgaagag ctgtatcaaa tcattacgga taccgaaggt actccggatg aagcgtagtt
    54781 attcggtaat gtctctttta gacgttgtga ggagaaacag tacatggtac tgatgatcgt
    54841 cagcggacgt tcaggttcag gtaaatctgt cgccctgcgt gcgctggaag atatgggttt
    54901 ttactgcgtg gataaccttc ccgtagtgtt gttacccgat ctggctcgaa ctctggccga
    54961 tcgcgagatt tctgccgccg tcagcattga tgttcgtaat atgccggagt caccagaaat
    55021 attcgaacag gcgatgagta acctgcctga cgctttctca ccgcaactac tgttcctgga
    55081 tgccgaccgt aataccttaa ttcgtcgtta cagtgacacg cgccgactgc atccgctttc
    55141 cagcaaaaac ctgtcgctgg aaagtgctat cgacaaagaa agcgatttgc tggagcctct
    55201 gcgttcgcga gcggatctga ttgtcgatac ctcagaaatg tccgttcacg agctggctga
    55261 aatgctgcgt acccgtctgc tgggtaaacg tgaacgcgaa ctgaccatgg tctttgagtc
    55321 tttcggcttc aaacacggta tccctatcga tgcagattac gtctttgacg tgcgcttctt
    55381 gccgaacccg cactgggatc cgaaactgcg tccaatgaca ggtcttgata aacctgtcgc
    55441 cgcgttcctc gaccgccaca cagaagtaca caattttatc taccaaacgc gaagctatct
    55501 tgagctatgg ttacctatgc tggaaaccaa caaccgtagc tacctgacgg tcgccattgg
    55561 ttgtaccggc gggaagcacc gttcggtgta tattgcagag caactggcag actacttccg
    55621 ctcgcgcggt aaaaacgtcc agtcacgcca tcgtacgctg gaaaaacgta aaccatgacc
    55681 gtcaagcaaa ctgttgaaat cacaaacaag ctgggcatgc atgcccggcc tgcaatgaag
    55741 ctgtttgaat taatgcaggg ttttgacgct gaagtgctgt tacgtaatga cgaaggcacc
    55801 gaggctgaag ccaacagcgt tattgcgctg ctgatgttgg attctgccaa aggacggcag
    55861 attgaagttg aagcgaccgg tccacaggaa gaggaagcac tggccgccgt tatcgccctc
    55921 tttaattctg gttttgatga agattaatct tcatcacttt gacatacaac tcccttcaag
    55981 ctcccccgac aataagaaaa tcacgtactg aaatcgttct caatcaacgt catttgtaca
    56041 ttttgtgcgc ttttcacttt cagaagaacc ttaagaaaac cttaagaggc attgtttagg
    56101 ttttgtttaa gttaatcgac catactggag atcgtcagaa aatatttcca ggagatggca
    56161 tgattcgctt atctgaacaa agtcccctgg gcaccggaag acatcggaaa tgttatgcgc
    56221 atccggaaga tgcccaacgc tgtatcaaga ttgtctacca tcgtggcgat ggtggcgata
    56281 aagagatccg ccgtgagtta aagtactacg cgcatcttgg tcgccggtta aaagactgga
    56341 gtggaatacc gcgctatcac ggtaccgttg agacggattg cggaaccggt tatgtctacg
    56401 atgtgattgc cgattttgac ggcaagcctt ccattacgct gaccgaattt gctgaacaat
    56461 gccgttacga agaagacatt gcacaactgc gccagctact gaaacagctg aagcgttatt
    56521 tgcaggataa ccgtatcgtg acgatgtcgc tgaagccgca gaatatcctc tgtcaccgca
    56581 tcagtgagtc agaggttatc ccggtcgtct gcgacaacat tggtgaaagc acattgattc
    56641 cactggcgac ctggtcgaag tggtgttgtc tgcgtaagca agagcggtta tggaaacgat
    56701 ttatcgccca accggcgctg gctattgccc tgcagaaaga tctgcaacct cgtgagagca
    56761 aaacgctggc cctcacttcg cgcgaagctt aatccagctg gtgttgttgc ataaacggct
    56821 caccgcctaa ctgatacatc tgccgtaaaa tccacgcctg acggctacgc acgtagccgt
    56881 caggcgtgga gactttgaaa cgaagtggat taggtaatac agctgccagt aatgcagctt
    56941 ccgaccgggt aagtttgctc gcgggtttgt ggaaataacg ttgtgccgca gcttcgacgc
    57001 caaacacacc gtcgccaaat tcggcgatat tcaggtaaac cgtcaggata cgctttttgc
    57061 tccagactgt ttctatcccc agcgttaatc cagcttccag cccttttcgt acccagctac
    57121 gcccatccca taaaaagaga tttttggccg tctgctgaga aatcgttgaa gcaccgcgaa
    57181 tacggttttc attgcgctcg ttgtgcgcca gggctttctc aatggaagcg acatcaaagc
    57241 cccagtgctc aggaaatttc tgatcttctg cggcaatcac cgccagtccc atccacggcg
    57301 agatttgatc catactgacc cagtcagaat gtgccacgta acgaaaattg ccatgcagcc
    57361 aggcgctgac ctgtcgctcg accattaccg ctgagaaggg aacaggcgca acgctaaaca
    57421 acgcgatgcc cccgccccag aaaacggcga ggacaaccat taaccgcaaa agaaaacggc
    57481 gaacgaaact aaacaccgtt aagcggcttt tactcattca gccagaacca gcacgcggga
    57541 aaccagctta tcaatgccgc tcgccgcttc tgcaatgttc tgcgccagca tatatgctgg
    57601 ggtggtgaca atcttattgt cttcatcaac cacgatatca tcgacaggac acggcacatg
    57661 ctccgcgccc atctcttcca gtacttctgc ggtatcgata tcagtaccga tggtcaaacg
    57721 cagcgggaaa tcgaaaattt tcggcagcat cgccggggca atacacataa aaccaagcgg
    57781 ttttccggcc tgatgcatcg cttgtgccag cgcctttaat tcacggtcaa cggtgcattc
    57841 gctaccaaga ctggcaaaat tgcttaaatt cttcgccgcg ccaacccccc ccggcacaat
    57901 caacgcatcc agttcagcgg catcggcctg ggccagagga cggatttcac cgcgcgttat
    57961 tcgtgccgct tcaatcagca cattgcgcgt ttccgtcatc gcttcgccag ttaaatggtt
    58021 gataacatca acctgctgct tatccggtgc aaagcagacc gcctgcgcac cgctgcgtga
    58081 aatagctaac aacgtcaaca ccgcttcatg aatttcagaa ccgtcataga cgccgcatcc
    58141 gctcagaatt acgccaattt tcttcattgt gatcatcctt tcgcaattga ctgaaacaca
    58201 ttaatttttt taataaaaat ggtacgcatc acacatttaa ctgattcatg taacaaatca
    58261 tttaagtttt gctatcttaa ctgcgtgcgg cctgaaaaac agtgctgtgc ccttgtaact
    58321 catcataata atttacggcg cagccaagat ttccctggtg ttggcgcagt attcgcgcac
    58381 cccggtctag ccggggtcat tttttagtgg cttttgccac ccacgctttc agcacttcta
    58441 cgtcgtgacg ccactcttct ttcatctctt caatccattc accgacgtta tcttcccagg
    58501 cgggaaggtc aggagactga atttgctgac ccagctgttg cagatggcgt aaccccactg
    58561 accccgccgc acctttaatt ttatgtcctt cctcaacaat gcctttttta tcctgcgccg
    58621 tcagattcga ctccagcacg ctgacatagc ccggcatcat cttctcaaac accgctaacc
    58681 cgtcggtgat cagcttcggt cctacaagtt cgagatactg ttccagcatg ggaatatcga
    58741 gcaatgcttc tgatttactg ttctcttctg tcgtcaccgt actctcctca tcatcctggg
    58801 tatcccagaa tttcttgatc atcgcggtta gcgccggaac cgaaagcggc ttactcagca
    58861 catcatccat tccagcattg aggtactctt gtttgtcttt cagcacgtta gcggttaagg
    58921 ccaccagcgg cggtaaatcc tcgcgcggat aacgcttcgt cagttcacga gagatatcca
    58981 gcccggtcat atctggcaac tgaatatcca gcaacaccag gtcgtattcg cccggtttaa
    59041 acatctccag cgccgccttg ccggtcatgg cgacatcaac gctgttacct aatttttcca
    59101 gcacagaacg cgcgacaatc acgttcagtt caatgtcttc caccagcagc acattcagcg
    59161 ccggtaaagg catatcgtct tcatcaaacg catcatcgac ctcttctgcc accgacggtg
    59221 cgtggatcgt caacgtaaag gttgaacctt tgccctgttc gctggtaacc gtaatatcgc
    59281 cgcccatatt tttcgccaga cgatgagaaa cggccagacc aataccggtg ccggtggcag
    59341 gtttaccgcc atgactgtct ttcacctggt aatacatggc gaagatttta tccagctcat
    59401 cctgcggaat gccgatacca gaatcttcca cttcaaaatg cagcatatcg ccttcatcgt
    59461 agcgcacgcg cacggtaacc tggccttgct gggtgaattt gacggcgtta ctgatgaggt
    59521 tccacaggat ctgccgtaaa cgcgtcccgt cggtaatgac ctgatgcggt aatggcagcg
    59581 taggctccag gttaaagcgc aatccttttt gttgcgcctg caatgcggag agattttcca
    59641 gatcggcaag gaagctggtg aaatcaaccg gctgattatc aagctggacc ttgcgccgtt
    59701 ccatcttatc catgtcgata atatcgttaa agatattccc cagcgtgacg gccgaaacat
    59761 ggatagtttt gagatatttt tcctgctcgg cggtgagttc ggtatccagc agaatgcggc
    59821 tcaggccgac gataccgttc agcggtgtac gcaattcgtg actgatggtg gaaataaacg
    59881 tcgttttgtc gcggctggcg cgttcaagcg catcctgata ccgcttacgc tcggtaatgt
    59941 cgcgaccaaa gcccatcaaa ccgtgacgtt tacccacgcg gtcgtagtac ggcactttac
    60001 ggatttcaaa gcaggctttg cgcccgtccg ggtaatccag ccactgttca taggtcagtg
    60061 acacattatg acggaacact ttttcatcgg tttcaatgac ttttgcggcg gcttccggtg
    60121 agtaaacatc agcaggtttc agatgaacca gttgtttttc gctttttccg gtcagcagct
    60181 ccatcgcgcg gttacagccg gaaaactctt tatcttcgtt acgataaaaa accaggtcgg
    60241 gtgaagcatc aaggaaggaa cgtaagaatg aggattgctg ctcgagctga atttgtgtct
    60301 cttcgcgctc tttgatttca attttcagtt ggccgaaggt ttcctgcagt tctgcttccg
    60361 ctttttcacg aacggcaatt tcctgattta gctgggcaat attatctttt aactgaacgt
    60421 tgaggctcaa atcgcgctcg cgcatctcct ccagtttttg caccagccgt gacagacgtt
    60481 gtcgtgactc ctccagttgc tcgacgacca ccgatagaaa gtagaccgcc cacggcgtaa
    60541 tcagcaaacc aaagaagata gaacgaataa catcaatgct ttcgacctga ccatgcagca
    60601 ccatggttac cgccatttgt accacaatgg caagaacgac gagggccagc gccagcaaca
    60661 ttgagaagcg caccagacct aacttcatca tcaggtcaac ataatactgc gccagcagac
    60721 gaatttgctt cattagggaa ttccttcacg acaacctgcc ccaataatac ccaattctga
    60781 cagttacgtt ataaattgtg tgagaaatgc ggaattcccc tcaacgacct actccgttgg
    60841 aggtagccac ggacgtccca gcgccgatcc ctgtacgcca tgttcattca gatagcgatc
    60901 cagctcgacc attcccgtcc atcgattttc gcaccacagc ggagcaagca gcgtcgggcg
    60961 acgggcgctg gcggaaatgc ggtggtagat cacttccggt ggcgtatggc gaatcatttc
    61021 tccggcagtg agcgtataat cctccagttc aataccgttc aaacgccccg cttcccaggc
    61081 tttcgccata atgctgcctt tcacaatatg cagcggatgc agctttatgc cgtccacgct
    61141 ggtttcaacc actcgctcca gcgtttgcaa acattcagcc tgcccttcgc caggcaggcc
    61201 gacaattaag tgggaacaca ccttcagccc gcgctgacgt gccagctggg ttgtacgctg
    61261 ataacaggca aaatcatggc cgcggttgat gcgatgcagt gttttgtcgt gggcggtttg
    61321 tagccccagc tccagccaca cttcgtaacc ctggtcctta tattcgcaaa gcagatccag
    61381 caccgcatcc ggcacgcagt ccgggcgggt accaacacac aaaccgacaa tattggcctg
    61441 gctcaccgcc tgctgataca tagaacgcag cacctgaact tccgcaaagg tgctggtata
    61501 cgcctgaaag taggccagat agcgtttagc gcggttaact aaattcgcct ggtgcgccag
    61561 ttgctcggca atggaacgat gctgctgcgc ttcatcggca aacgaggcaa cattacagaa
    61621 tgtgcagcca ccacgcccga tggtaccgtc acggttaggg cagctaaaac cgccgtgcag
    61681 cgtcagctta tgcacctttt gcccataacg acgggtgaga tcaccaccaa acatattgac
    61741 taatttctgt aactgcataa tctgatagac cgcgccttga aaagaggcca aagcctgcca
    61801 tttttagctc aattcggcga tgacctagat caatcgtccc ggcctgcttt tatcaactgc
    61861 ataatcaatc aaaattaccg aaatttcatg cataatcaca taaatcactt ttgcttatct
    61921 tgtgtcagat ttttttatct ccttatggat tttaggcaaa aacagtagca tgaaacgtca
    61981 ttaccaatta aggcagtata aaatgctggt tttctcgtca gttcaaggca ggataagggt
    62041 taacacgcct ttatgacagt caggaattga ctgtttctct aacgactctc cttttagcct
    62101 taaagataaa atctatttta atttcagcca tttaataaag aattttgcgc taaagcacat
    62161 ttctgtacca ataagcttgc catttgacct gtatcagctt tcccgataag ttggaaatcc
    62221 gctggaagct ttctggatga gcagcctgct catcatattt atgcagtaat tgagatcccc
    62281 tcttcaccgt attaaccgat gcgaaaagga caacaagggg gcgaatgcga ggcgcgcgta
    62341 tgacacgcaa accccgtcgc cacgctcttt ctgtgcccgt gcgcagcggt tcggaagtgg
    62401 ggttcccgca gagcctgggg gaggttcacg atatgttgta cgataaatcc cttgagaggg
    62461 ataactgtgg tttcggcctg atcgcccaca tagaaggcga acctagccac aaggtagtgc
    62521 gtactgcaat acacgcactg gcccgcatgc agcaccgtgg cgcgattctc gccgatggta
    62581 aaaccggcga cggttgcggc ttgctgttac aaaaaccgga tcgctttttt cgcatcgttg
    62641 cgcaggagcg cggctggcgt ttagcaaaaa actacgctgt cgggatgctc ttcctgaata
    62701 aagatcctga actcgccgct gccgcacgcc gcatcgttga agaagaactg caacgcgaaa
    62761 ccttgtcgat tgtgggctgg cgtgatgtcc ccactaacga aggcgtgctg ggtgaaatcg
    62821 ccctctcctc tctgccacgc attgagcaaa tttttgtgaa cgccccggca ggctggcgtc
    62881 cacgcgatat ggagcgccgt ctgtttatcg cccgccgccg cattgaaaag cgtctcgaag
    62941 ccgacaaaga cttctacgtc tgtagcctgt cgaatctggt gaacatctat aaaggtctgt
    63001 gtatgccggc ggatctgccg cgcttttatc tggatcttgc ggacctgcgt ctggaatcgg
    63061 ccatttgcct gttccaccag cgcttctcca ctaacaccgt accgcgctgg ccgctggcgc
    63121 aaccgttccg ctatctggcg cataacggtg aaatcaacac catcaccggt aaccgccaat
    63181 gggcgcgtgc gcgtacctat aaattccaga cgccgctgat ccctgacctg cacgacgccg
    63241 cgccattcgt caacgaaacc ggttctgact ccagttccat ggataacatg ctggaactgc
    63301 tgctggcagg cgggatggat atcatccgcg ccatgcgcct gttagtgcca cccgcctggc
    63361 agaacaaccc ggatatggac ccggaactgc gtgccttctt tgactttaac tccatgcata
    63421 tggagccgtg ggatggtccg gcgggcatcg tgatgtccga tggtcgcttc gctgcctgta
    63481 acctcgaccg taacggtctg cgtccggcac gctacgtcat taccaaagac aagctcatca
    63541 cctgcgcctc tgaagtcggt atctgggatt accagcctga cgaagtggtc gaaaaaggcc
    63601 gcgtcgggcc aggcgaactg atggtgatcg acactcgcag tgggcgtatt ctgcactcgg
    63661 cagaaaccga tgacgatctg aaaagccgcc acccttataa agagtggatg gagaaaaacg
    63721 tccgccgact ggtaccgttt gaagatctgc ccgatgaaga agtgggtagc cgcgaactgg
    63781 acgacgacac gcttgccagc taccagaaac agtttaacta cagcgcggaa gagctggact
    63841 ccgtaattcg cgtactgggc gaaaacggtc aggaagcggt cggttcgatg ggcgatgata
    63901 ccccattcgc cgtgctctcc agtcagccgc gcattattta cgactacttc cgccagcagt
    63961 ttgcccaggt gactaacccg ccaatcgacc cgctgcgtga agcgcatgtt atgtcgctcg
    64021 ccaccagtat cggtcgtgaa atgaacgtct tttgcgaagc agagggccag gcgcaccgtt
    64081 taagctttaa atcgccgatt ctgctctact ccgatttcaa acagctcacg acgatgaaag
    64141 aggagcacta ccgcgcagat acgctggata tcacctttga cgtcactaaa accacgctcg
    64201 aagcgacagt caaagagcta tgcgacaaag ccgaaaaaat ggtacgtagc ggcaccgtgt
    64261 tgctggtgct ctccgaccgg aatatcgcta aagatcgcct gccggttccg gctccgatgg
    64321 cggttggcgc gatccagacc cgtctggtcg atcaaagcct gcgttgcgat gccaacatca
    64381 tcgtcgaaac cgccagcgcc cgcgatccgc accacttcgc cgtgttgctg ggtttcggcg
    64441 cgacggctat ttatccgtac cttgcctatg aaacgctggg ccgcctggta gacacccacg
    64501 cgattgccaa agattatcgt accgtgatgc tcaactaccg taacggcatc aacaaaggct
    64561 tgtacaaaat catgtccaaa atgggcatct ccaccatcgc ctcttaccgc tgctcgaaac
    64621 tgtttgaagc ggtcggtcta cacgatgatg tagtgggcct gtgcttccag ggcgcggtca
    64681 gccgcattgg tggggcaagc tttgaagact tccagcagga tctgctgaat ctgtcgaaac
    64741 gtgcctggct ggcgcgcaag ccgattagcc agggcggcct gctgaaatac gtccacggcg
    64801 gcgaatacca cgcctacaac ccggacgtgg tgcgcacgct gcaacaggcg gtacaaagcg
    64861 gcgagtacag cgactatcag gaatacgcga agctggttaa tgagcgtccg gcaaccacgc
    64921 tgcgcgatct gctggcaatt acgccgggtg aaaacgcggt caacattgct gatgttgaac
    64981 cggcaagcga actgtttaaa cgctttgata ccgccgcgat gtctatcggc gcgttaagcc
    65041 cggaagccca cgaggcgctg gcggaagcga tgaatagcat cggcggtaac tcgaactccg
    65101 gtgaaggcgg cgaagacccg gcgcgctatg gcactaacaa agtgtcgcgc atcaagcagg
    65161 tggcttctgg tcgcttcggc gttactccgg cgtatctggt caatgccgac gtcattcaga
    65221 ttaaagtcgc ccagggcgcg aagccgggcg aaggcggtca gttgccgggc gacaaagtca
    65281 ctccttacat cgccaaactg cgctattcgg tgcccggagt gacgctgatc tccccgccgc
    65341 cgcaccacga tatctactct atcgaagact tagcgcagct tattttcgac ctcaagcagg
    65401 ttaacccgaa agcgatgatc tccgttaagc tggtgtccga accaggcgtt ggcaccatcg
    65461 cgactggcgt ggcgaaagct tatgcggact tgatcaccat cgcgggctat gacggcggca
    65521 ccggcgcaag cccgctgtcg tcagtgaaat atgcaggctg cccgtgggaa ctcggtctgg
    65581 tggaaaccca acaggcgctg gttgccaacg gcttgcgtca taaaattcgt ttgcaggttg
    65641 atggcggcct gaaaacgggt gtcgatatca tcaaagcggc gattctcggc gcagaaagct
    65701 tcggcttcgg cactggccca atggtggcgc tcggctgtaa atatctgcgt atttgccacc
    65761 tgaacaactg cgccacgggt gtagcaactc aggatgacaa actgcgtaag aatcactatc
    65821 acggcctgcc gttcaaggtg actaattact ttgagtttat cgcccgtgaa acccgcgagc
    65881 tgatggcaca gctgggagta acgcgtctgg tggatctgat tggtcgcacc gacctgctga
    65941 aagagctgga cggtttcacc gccaaacagc agaaactggc gctgtcgaag ctgctggaga
    66001 ctgccgagcc acatgcaggt aagtcactct actgcactga aaacaacccg ccgtttgata
    66061 acggcctact gaacgcgcag ttgctgcaac aggcgaaacc gtttgtcgat gaacgccaga
    66121 gcaaaacctt ctggttcgat atccgcaaca ccgaccgttc tgtcggcgcg tcgctttcag
    66181 gctatatcgc ccagacgcac ggcgatcagg ggctggcagc cgatcctatc aaagcgtact
    66241 tcaacggcac cgcaggccag agtttcggtg tgtggaacgc gggcggcgtg gaactgtacc
    66301 tgaccgggga tgccaacgac tatgtcggta aaggcatggc gggcggctta atcgccattc
    66361 atcctccggt tggttccgcc ttccgcagcc atgaagcaag cattatcggc aacacctgcc
    66421 tgtatggcgc gaccggtggt cgtctgtatg ccgcaggccg cgcgggtgaa cgtttcggcg
    66481 tgcggaactc cggtgctatc accgtggtag aaggcattgg cgacaacggc tgtgaatata
    66541 tgacgggcgg tattgtctgt attctgggta aaaccggcgt taacttcggc gcgggcatga
    66601 ccggcggctt cgcttacgtt ctcgatgaaa gcggtgattt ccgcaaacgc gttaacccgg
    66661 aactggtcga agtcttaagc gtcgacgatc tggcgatcca tgaagagcat ctgcgcggtc
    66721 ttatcaccga gcatgttcag cataccggtt ctcagcgcgg tgaagagatt ctggcgaact
    66781 ggtcaacctt cgccacgaaa ttcgcgctgg ttaaaccgaa gtccagtgat gtaaaagcac
    66841 tgctgggtca ccgtagtcgt agcgcagcag agttgcgcgt gcaggcgcag taaggggtag
    66901 caacaatgag tcagaatgtt tatcaattta tcgacctgca gcgcgttgat ccgccaaaga
    66961 aaccgctgaa gatccgtaaa attgagtttg ttgaaattta cgagccgttt tccgaaggcc
    67021 aggccaaagc gcaggctgac cgctgtctgt cgtgcggcaa cccatactgc gagtggaaat
    67081 gcccggtaca caactacatc ccaaactggc tgaagctcgc caacgagggg cgtatttttg
    67141 aagcggcaga actgtcgcat cagaccaaca cactgccgga agtatgcgga cgtgtctgcc
    67201 cgcaagaccg tctgtgcgaa ggttcctgca ccctgaacga tgagttcggt gcggtgacca
    67261 tcggcaacat tgagcgttat atcaatgata aagcgttcga aatgggctgg cgtccggata
    67321 tgtccggcgt gaagcaaacc ggtaaaaaag tggcgattat cggtgcaggc ccggcaggtc
    67381 tggcgtgtgc ggatgtcctg acgcgtaacg gcgtaaaagc cgttgtcttc gaccgtcatc
    67441 cagaaattgg cgggctgctg accttcggta ttccggcctt caagctggaa aaagaggtaa
    67501 tgacgcgtcg ccgtgaaatc ttcaccggca tgggtattga attcaaactc aataccgaag
    67561 tgggccgcga cgtacagctg gacgatctgc tgagtgatta cgatgccgtg ttccttggcg
    67621 ttgggactta tcagtcaatg cgcggcgggc tggaaaacga agacgccgat ggcgtgtacg
    67681 cagcgctgcc gttccttatc gccaacacca aacagttaat gggctttggt gaaacccgcg
    67741 acgaaccgtt cgtcagcatg gaaggcaaac gcgtggtagt acttggcggt ggcgacactg
    67801 cgatggactg cgtgcgcacg tctgtacgcc agggggcaaa gcacgttacc tgtgcctatc
    67861 gtcgtgatga agagaacatg ccgggttccc gccgcgaagt gaaaaacgcg cgggaagaag
    67921 gcgtagagtt caaattcaac gtccagccac tggggattga agtgaacggt aacggcaaag
    67981 tcagcggcgt aaaaatggta cgtaccgaaa tgggcgaacc ggatgccaaa ggccgtcgcc
    68041 gcgcggagat cgttgcaggt tccgaacata tcgttccggc agatgcggtg atcatggcgt
    68101 ttggtttccg tccacacaac atggaatggc tggcaaaaca cagcgtcgag ctggattcac
    68161 aaggccgcat catcgctccg gaaggcaacg acaacgcttt ccagaccagc aacccgaaaa
    68221 tctttgctgg cggcgatatc gtccgtggtt ccgatctggt cgttaccgcc atcgccgaag
    68281 gtcgcaaagc ggcagacggc attatgaact ggctggaagt ttaagctctg tcccagcgcc
    68341 gcctcctcag aaaagagtcg gcgctgaaat tctcctttct ttctggaaat cgcctcgcat
    68401 aatctgaaga aaaccaatgt aatacgctca tctggattgt taccggtaga tgcccggcgt
    68461 atagctgatt ccattattca acgcagcgcc atataaaggg acagataatg gaatcgctct
    68521 cagagagaac ctcaacaggc taccagcaaa tccacgacgg cattattcat ctggtcgata
    68581 gcgcccgaac ggaaacggta cgtagcgtta acgcgctgat gaccgcgacg tactgggaaa
    68641 ttggccgacg gattgtcgaa tttgaacaag gtggtgaggc cagagcagct tatggtgcac
    68701 agttgattga tcggctatcg aaggatttaa gtcgacggta taaacgtggg gtctctgcaa
    68761 aaaacttacg ccaaatgagg cttttttacc tcttttttca acatgttgaa attcgccaga
    68821 cagtgtctgg cgaattaaca ccattgggaa ttccgcagac accatctgca gaattcccat
    68881 cggcaaagat ttggcagacg ctgtctgcca aattgagagg ctgtttgata tttccacagc
    68941 ttttgtattg catctcctat tttcggtttg tatattcatg caaataaacg aagg
//
LOCUS       DADMEQ010000006        68192 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.006, whole genome shotgun sequence.
ACCESSION   DADMEQ010000006 DADMEQ010000000
VERSION     DADMEQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 68192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68192
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.006"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..247
                     /locus_tag="JNZ05_000410"
     CDS             <1..247
                     /locus_tag="JNZ05_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8563765.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="JNZ05_000411"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="JNZ05_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HAY8563766.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="JNZ05_000412"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="JNZ05_000412"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY8563767.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..3217
                     /gene="mdfA"
                     /locus_tag="JNZ05_000413"
     CDS             1991..3217
                     /gene="mdfA"
                     /locus_tag="JNZ05_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HAY8563768.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTMLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMVNVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3258..3542)
                     /locus_tag="JNZ05_000414"
     CDS             complement(3258..3542)
                     /locus_tag="JNZ05_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8563769.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="JNZ05_000415"
     CDS             complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="JNZ05_000415"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HAY8563770.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMKNAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4443..5651)
                     /locus_tag="JNZ05_000416"
     CDS             complement(4443..5651)
                     /locus_tag="JNZ05_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8563771.1"
                     /translation="MTVNSSRNALKRRTRALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAAAKPD
                     TKTQTAMENS"
     gene            5735..6271
                     /locus_tag="JNZ05_000417"
     CDS             5735..6271
                     /locus_tag="JNZ05_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY8563772.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6446..8131)
                     /locus_tag="JNZ05_000418"
     CDS             complement(6446..8131)
                     /locus_tag="JNZ05_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HAY8563773.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAITNVLLTLAGTIIVMVWPGLG"
     gene            8401..8778
                     /locus_tag="JNZ05_000419"
     CDS             8401..8778
                     /locus_tag="JNZ05_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8563774.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="JNZ05_000420"
     CDS             complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="JNZ05_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HAY8563775.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9225..9512
                     /locus_tag="JNZ05_000421"
     CDS             9225..9512
                     /locus_tag="JNZ05_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HAY8563776.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVVKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9496..10218
                     /gene="nfsA"
                     /locus_tag="JNZ05_000422"
     CDS             9496..10218
                     /gene="nfsA"
                     /locus_tag="JNZ05_000422"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HAY8563777.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="JNZ05_000423"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="JNZ05_000423"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HAY8563778.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="JNZ05_000424"
     CDS             11269..11745
                     /locus_tag="JNZ05_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HAY8563779.1"
                     /translation="MTSLVVPGLDTLRQWLDGLGMSFFECNNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..13209
                     /gene="potF"
                     /locus_tag="JNZ05_000425"
     CDS             12097..13209
                     /gene="potF"
                     /locus_tag="JNZ05_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HAY8563780.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13304..14380
                     /gene="potG"
                     /locus_tag="JNZ05_000426"
     CDS             13304..14380
                     /gene="potG"
                     /locus_tag="JNZ05_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HAY8563781.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPT"
     gene            14447..15400
                     /gene="potH"
                     /locus_tag="JNZ05_000427"
     CDS             14447..15400
                     /gene="potH"
                     /locus_tag="JNZ05_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HAY8563782.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15397..16242
                     /gene="potI"
                     /locus_tag="JNZ05_000428"
     CDS             15397..16242
                     /gene="potI"
                     /locus_tag="JNZ05_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HAY8563783.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16302..16790
                     /locus_tag="JNZ05_000429"
     CDS             16302..16790
                     /locus_tag="JNZ05_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HAY8563784.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16831..17958
                     /gene="rlmC"
                     /locus_tag="JNZ05_000430"
     CDS             16831..17958
                     /gene="rlmC"
                     /locus_tag="JNZ05_000430"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HAY8563785.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="JNZ05_000431"
     CDS             complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="JNZ05_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8563786.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19156..19824)
                     /gene="artM"
                     /locus_tag="JNZ05_000432"
     CDS             complement(19156..19824)
                     /gene="artM"
                     /locus_tag="JNZ05_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HAY8563787.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="JNZ05_000433"
     CDS             complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="JNZ05_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HAY8563788.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="JNZ05_000434"
     CDS             complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="JNZ05_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8563789.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21296..22024)
                     /gene="artP"
                     /locus_tag="JNZ05_000435"
     CDS             complement(21296..22024)
                     /gene="artP"
                     /locus_tag="JNZ05_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HAY8563790.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22242..22757)
                     /locus_tag="JNZ05_000436"
     CDS             complement(22242..22757)
                     /locus_tag="JNZ05_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY8563791.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     ADTLRQSIENR"
     gene            23406..25175
                     /locus_tag="JNZ05_000437"
     CDS             23406..25175
                     /locus_tag="JNZ05_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY8563792.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNAFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSIGARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25386..25709
                     /locus_tag="JNZ05_000438"
     CDS             25386..25709
                     /locus_tag="JNZ05_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAY8563793.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25706..26536
                     /locus_tag="JNZ05_000439"
     CDS             25706..26536
                     /locus_tag="JNZ05_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAY8563794.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26533..27546)
                     /locus_tag="JNZ05_000440"
     CDS             complement(26533..27546)
                     /locus_tag="JNZ05_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY8563795.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIAHSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27645..29075)
                     /locus_tag="JNZ05_000441"
     CDS             complement(27645..29075)
                     /locus_tag="JNZ05_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY8563796.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="JNZ05_000442"
     CDS             complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="JNZ05_000442"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HAY8563797.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGAHIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="JNZ05_000443"
     CDS             complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="JNZ05_000443"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HAY8563798.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="JNZ05_000444"
     CDS             complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="JNZ05_000444"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HAY8563799.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="JNZ05_000445"
                     /gene_synonym="priS"
     CDS             complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="JNZ05_000445"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HAY8563800.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPSFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="JNZ05_000446"
     CDS             complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="JNZ05_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HAY8563801.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="JNZ05_000447"
     CDS             complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="JNZ05_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HAY8563802.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            37227..38885
                     /locus_tag="JNZ05_000448"
     CDS             37227..38885
                     /locus_tag="JNZ05_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAY8563803.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(38882..39838)
                     /locus_tag="JNZ05_000449"
     CDS             complement(38882..39838)
                     /locus_tag="JNZ05_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HAY8563804.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            39989..41104
                     /gene="macA"
                     /locus_tag="JNZ05_000450"
     CDS             39989..41104
                     /gene="macA"
                     /locus_tag="JNZ05_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HAY8563805.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMGGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            41101..43047
                     /gene="macB"
                     /locus_tag="JNZ05_000451"
     CDS             41101..43047
                     /gene="macB"
                     /locus_tag="JNZ05_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HAY8563806.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(43120..43344)
                     /gene="cspD"
                     /locus_tag="JNZ05_000452"
     CDS             complement(43120..43344)
                     /gene="cspD"
                     /locus_tag="JNZ05_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HAY8563807.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            43667..43987
                     /gene="clpS"
                     /locus_tag="JNZ05_000453"
     CDS             43667..43987
                     /gene="clpS"
                     /locus_tag="JNZ05_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HAY8563808.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            44018..46294
                     /gene="clpA"
                     /locus_tag="JNZ05_000454"
     CDS             44018..46294
                     /gene="clpA"
                     /locus_tag="JNZ05_000454"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HAY8563809.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(46697..46784)
                     /locus_tag="JNZ05_000455"
     tRNA            complement(46697..46784)
                     /locus_tag="JNZ05_000455"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46748..46750),aa:Ser,seq:gga)
     gene            complement(47038..47256)
                     /gene="infA"
                     /locus_tag="JNZ05_000456"
     CDS             complement(47038..47256)
                     /gene="infA"
                     /locus_tag="JNZ05_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAY8563810.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(47541..48245)
                     /gene="aat"
                     /locus_tag="JNZ05_000457"
     CDS             complement(47541..48245)
                     /gene="aat"
                     /locus_tag="JNZ05_000457"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HAY8563811.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVCCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(48287..50008)
                     /gene="cydC"
                     /locus_tag="JNZ05_000458"
     CDS             complement(48287..50008)
                     /gene="cydC"
                     /locus_tag="JNZ05_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HAY8563812.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(50009..51775)
                     /gene="cydD"
                     /locus_tag="JNZ05_000459"
     CDS             complement(50009..51775)
                     /gene="cydD"
                     /locus_tag="JNZ05_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HAY8563813.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(51898..52863)
                     /gene="trxB"
                     /locus_tag="JNZ05_000460"
     CDS             complement(51898..52863)
                     /gene="trxB"
                     /locus_tag="JNZ05_000460"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAY8563814.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            53408..53902
                     /gene="lrp"
                     /locus_tag="JNZ05_000461"
     CDS             53408..53902
                     /gene="lrp"
                     /locus_tag="JNZ05_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HAY8563815.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            54037..58143
                     /gene="ftsK"
                     /locus_tag="JNZ05_000462"
     CDS             54037..58143
                     /gene="ftsK"
                     /locus_tag="JNZ05_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY8563816.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAVDPDDVLFLGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSIFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFTQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            58298..58909
                     /gene="lolA"
                     /locus_tag="JNZ05_000463"
     CDS             58298..58909
                     /gene="lolA"
                     /locus_tag="JNZ05_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HAY8563817.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDEQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            58920..60263
                     /locus_tag="JNZ05_000464"
     CDS             58920..60263
                     /locus_tag="JNZ05_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAY8563818.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            60354..61646
                     /gene="serS"
                     /locus_tag="JNZ05_000465"
     CDS             60354..61646
                     /gene="serS"
                     /locus_tag="JNZ05_000465"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAY8563819.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            61886..64330
                     /gene="dmsA"
                     /locus_tag="JNZ05_000466"
                     /pseudo
     CDS             61886..64330
                     /gene="dmsA"
                     /locus_tag="JNZ05_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            64341..64958
                     /gene="dmsB"
                     /locus_tag="JNZ05_000467"
     CDS             64341..64958
                     /gene="dmsB"
                     /locus_tag="JNZ05_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAY8563820.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            64960..65823
                     /locus_tag="JNZ05_000468"
     CDS             64960..65823
                     /locus_tag="JNZ05_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase"
                     /protein_id="HAY8563821.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(65859..66485)
                     /locus_tag="JNZ05_000469"
     CDS             complement(65859..66485)
                     /locus_tag="JNZ05_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY8563822.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            66800..67948
                     /locus_tag="JNZ05_000470"
     CDS             66800..67948
                     /locus_tag="JNZ05_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8563823.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGSVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT        16935 a        16868 c        17862 g        16527 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttacactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccatg ttgcgcttct tgcagggcat
     2341 aagcctctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagatcaccg cgctgatggt gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccac
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat
     2701 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
     2761 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
     2821 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
     2881 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
     2941 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
     3001 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
     3061 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
     3121 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
     3181 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
     3241 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
     3301 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
     3361 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
     3421 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
     3481 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc
     3541 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc
     3601 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac
     3661 tttatcgatc acatccagta cgccttcacg gttattggag cctgcccggt attttgccgc
     3721 tgcgacgacc gcgctgccgg catttttcat tgcaaaacta aagcctgcct gacgcagcat
     3781 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
     3841 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
     3901 gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg
     3961 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa
     4021 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
     4081 ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac
     4141 ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc
     4201 attaaaaaca tctttgcctt cactcactac ccagccgccg ttttcagcca caaaggcaat
     4261 ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
     4321 cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
     4381 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatact
     4441 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcggc ggctttcgcg
     4501 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
     4561 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
     4621 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
     4681 agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag
     4741 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga
     4801 accacggcaa cgcgactgta acggtcgatg aaccaaccgc cggtgaagcg tccaacggtc
     4861 atccccaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca
     4921 tcaaccatta ataagggtaa ccagtcgttt gcagaacctt cggcaaaggc catcgccagc
     4981 actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca
     5041 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
     5101 taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
     5161 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
     5221 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
     5281 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
     5341 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac
     5401 atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
     5461 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
     5521 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
     5581 ggcaaaaaga agaacataaa cagcgccctg gttcggcgtt tcaatgcatt acgtgaagaa
     5641 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
     5701 acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
     5761 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
     5821 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
     5881 ttcaggaatt gatgagttgt tgctggaagc gttcagcagt tttactgaga ttatgtcccg
     5941 gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac
     6001 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
     6061 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
     6121 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
     6181 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
     6241 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
     6301 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
     6361 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
     6421 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
     6481 tccctgccag cgtcagcagg acgttggtga ttgcgtaggt gcccgcatag cccagcgcag
     6541 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
     6601 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
     6661 aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa
     6721 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
     6781 ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg
     6841 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
     6901 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
     6961 caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga
     7021 tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt
     7081 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
     7141 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt
     7201 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
     7261 ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
     7321 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
     7381 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
     7441 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
     7501 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
     7561 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
     7621 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
     7681 gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg
     7741 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
     7801 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
     7861 ttttcccatc gcgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
     7921 aaataaacag cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
     7981 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
     8041 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
     8101 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
     8161 tgttgaaaga aatggtaatt tacgataatg tgttttacca gaattcaggg tgcagattca
     8221 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
     8281 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
     8341 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
     8401 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
     8461 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
     8521 ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa
     8581 gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg
     8641 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg
     8701 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
     8761 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
     8821 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
     8881 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
     8941 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
     9001 tctgccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt
     9061 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
     9121 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
     9181 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
     9241 aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc
     9301 tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
     9361 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
     9421 aggtggtaaa acgtcttgcc ccacagctga tgaaccgtcc accgcaatat tcacgttcag
     9481 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg ctccattcgc
     9541 catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt
     9601 gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca
     9661 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
     9721 ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
     9781 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
     9841 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgccacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgttacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg gcctggggat gagttttttt
    11341 gaatgtaata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgccgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12361 cttgaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12421 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12481 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12541 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12601 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12661 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12721 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12781 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12841 tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12901 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgcctgcgga tgccaaaaac
    12961 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    13021 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    13081 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    13141 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13201 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13261 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13321 tcgcccgcag gcgaaaaccc gtaaggtgct gacgccgcta ttagaaatcc gcaacctcac
    13381 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13441 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    13501 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    13561 tccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat
    13621 gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat
    13681 ttccagtcgg gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa
    13741 accgcatcag ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    13801 gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga
    13861 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    13921 cacccacgat caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg
    13981 gaaatttgtc cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag
    14041 cgccgaattt attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga
    14101 tggcctggtg cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc
    14161 ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga
    14221 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    14281 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gcgcccagct
    14341 acaaaacgcc catcgtcatc gtaaagggtt accgacctag ggcgacgaag tgcgtttgtg
    14401 ctgggaagtg gacagcagtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    14461 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc agctgcaaat
    14521 gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct
    14581 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    14641 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    14701 ttttctgcaa ctgaccgacg atccgctcta tttcgatgct tatctccagt cgctacaggt
    14761 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    14821 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    14881 ctcgtttctg atccgcgttt atgcctggat gggaatatta aaaaacaacg gtgtgctgaa
    14941 taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct
    15001 ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac
    15061 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    15121 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    15181 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    15241 agacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    15301 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    15361 ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    15421 tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    15481 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggc cggctggtca
    15541 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc
    15601 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    15661 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    15721 ccgctggtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    15781 gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc
    15841 actttctgta cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc
    15901 tcgatagaag aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    15961 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    16021 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    16081 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg
    16141 atcctcggcg cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa
    16201 aaacagcgga tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca
    16261 acctggctac ataatgccgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg
    16321 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    16381 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    16441 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    16501 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    16561 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    16621 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    16681 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    16741 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    16801 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    16861 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    16921 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    16981 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca
    17041 ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat
    17101 ccagcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta
    17161 acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    17221 cagagtgatg gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa
    17281 ctgcgtaagg cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc
    17341 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa
    17401 caacaggcac tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    17461 ttccagacta atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga
    17521 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac
    17581 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt
    17641 gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc
    17701 actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc
    17761 cgcggcattg gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc
    17821 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    17881 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg
    17941 ctgctggtga agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct
    18001 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa
    18061 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    18121 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    18181 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    18241 agcgataccc aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc
    18301 cagttgcgga ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc
    18361 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    18421 cacttccggg tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat
    18481 gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggcaa tcacgacggc
    18541 tgagttttca tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc
    18601 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    18661 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    18721 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    18781 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    18841 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    18901 ccgtgtctgc ggcatgggat aacaatgcca ccaagtgaat ttatatgcaa taaacatgat
    18961 taaataattt aaatgaaata aaaaaagacg gataacttag tgagttgtcc gtcttcatta
    19021 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
    19081 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga
    19141 atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg
    19201 catcatcagc gtcagcaggc cgttgacgac caggtaaata atccctgccg caccgaacac
    19261 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
    19321 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    19381 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
    19441 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
    19501 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
    19561 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaactggc ccggcccgta
    19621 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
    19681 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    19741 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    19801 cagctcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    19861 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    19921 acaatgtacc aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt
    19981 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    20041 ttaccgaggc caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
    20101 gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc
    20161 aacgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    20221 ccacaaagga acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    20281 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa
    20341 tagataaaca gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    20401 cctgcccacg cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc
    20461 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    20521 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    20581 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg
    20641 ttgccctgac gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta
    20701 tcgcccactg ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca
    20761 ccaaatacgc tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
    20821 tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg
    20881 ttctgaacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    20941 cccacaaaca gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc
    21001 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    21061 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt
    21121 gcctgtgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    21181 ggaggatagg aggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
    21241 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    21301 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    21361 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
    21421 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    21481 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
    21541 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    21601 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
    21661 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
    21721 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    21781 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
    21841 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    21901 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
    21961 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    22021 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga
    22081 cagaataatt atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa
    22141 acatagcaca aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga
    22201 aatgccggat aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
    22261 tgacggagcg tatccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    22321 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt
    22381 ttgagatcca cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg
    22441 gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    22501 tcacgatgac tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa
    22561 taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt
    22621 tgagccacac tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta
    22681 taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat
    22741 agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc
    22801 aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac
    22861 ctgattgggt ttgagtccat tgctggctac ccactcaagg ttgctcgtag ctgacttact
    22921 taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct
    22981 gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag
    23041 aggttaagag catctgcaag aagccagttt cacatttata acgcgtgata aatgaatgcg
    23101 gttattgtct aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg
    23161 attgccaaaa ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac
    23221 tctcgataat attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag
    23281 tgaagaataa attaaattgc ttcagtattt cgttaattaa tatgaataca aaaggcgttg
    23341 tgcttttata attataaagc gcgaggagtt cggtggatta tcgtttttct tggatgattt
    23401 ttaatatgat aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc
    23461 aaagtgataa tttcctggat tcactgaggg aatttttcgc gatattaaat tccagtcgta
    23521 agggtgagtt gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg
    23581 acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc
    23641 aatttaatac attttttagc aatgctttcg agattgatgt taaaaaatat ctgggtaaat
    23701 cagaagataa cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta
    23761 atgtatcgaa tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata
    23821 aattacaaga acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt
    23881 ataccattgg tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact
    23941 ttaacaaaga acaatatata tcctttataa aggacgcatt tattcatttt tatgattata
    24001 gcaggcgata tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat
    24061 tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta
    24121 cagaagaaga gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg
    24181 ctgatgataa ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg
    24241 cctttcaaga taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata
    24301 tttgtagcgt aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga
    24361 atgaagaagt tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa
    24421 gtaatttagt caatcttaaa acactactca atgatggttg ttttattaaa tatagtcatt
    24481 ttaatgacgt ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg
    24541 tgaatgaaag catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa
    24601 taacaaatag caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa
    24661 ttaataacgt aaatactcat tctttaaaat ttattaactg caattttttc aatgttgata
    24721 tgataagagt taatctcagc aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga
    24781 taaaaccctg gttgcctgta aaatggacaa aagaattaat aagtgattat ttgtatggct
    24841 gtttactatc tctgtactct atcggtgcaa gggatatata taatatgaac gccgggaata
    24901 acgtgaaggt tgctgcggat gctttccttg aaattatttt ctctcttaaa aataaatatt
    24961 gtattaaact attaagcgct caggatcggg cgtttatata cgagtttgca agaatgatct
    25021 tcgcttatat taatgacaaa agcattgaga tcttattact ttcttgtttt gcggcggctg
    25081 accaaaaagc aatccagcgg tatagacctc agtcacaaga tggggaagac tttaggagtc
    25141 atttacaata taagttgcct ctttccgctc attaatgttg ttggtgcaac ataataagac
    25201 aggtaagcgg taccctataa tgaaaccaat atcgggttat gcaacggata atcgtaaaga
    25261 cttaagtggg tgtttttgac acaatgagat gatttcctaa actgatcaaa accttcttcc
    25321 caacatggca tatcagtccg ataatgcttt agagatcgaa ggcttaacaa acaaggagat
    25381 cgatcatgca attttctaca accccaactc tggaaggcca gaccatcgtt gaatattgcg
    25441 gtgtggtgac cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta
    25501 tccgcgatat cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg
    25561 agatcgcctt tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtagttg
    25621 gtattgatat cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca
    25681 gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct
    25741 gttactggca gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga
    25801 tacccgacac caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac
    25861 cgcagatgat tttgatagct cgctggcgac actgactgat aagcaggtca gctcgcatta
    25921 tctgatccct gcggtgccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc
    25981 ggaacaagaa ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa
    26041 cgacacctct attggcattg agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa
    26101 atattttgcc ccgtttgaac aggcacagat tcaggcgctt attccgctgg cgaaagatat
    26161 tattgcccgt tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca
    26221 gcgcaaagac gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg
    26281 tgcctggccg gatgcgcagc gggtgaactt ttaccttgcc gggcgcgcgc cgcacacacc
    26341 ggtagatacc gcgtcattgc tggagctttt ggcgcgctac ggttatgacg taaaacctga
    26401 tatgacaccg cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac
    26461 gttatataac ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa
    26521 atacgggcag gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc
    26581 gatgccttca tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat
    26641 atccagcgta aagtcaaaat tcaacttgga gacgccgtag tgggtcagcg gcggctcttt
    26701 tgctgacttg cggcctaaac gctccatgct gtgggcgatc atatccagca tcgggtaagg
    26761 gacggaacga atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg
    26821 cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca
    26881 ggcttcctgg cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac
    26941 cagcgcactg ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag
    27001 acggggaata aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa
    27061 gcgcgtttgt ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct
    27121 gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct tctttaatat cgcgatggtg
    27181 gtgataatcg aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc
    27241 gacagcccat tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg
    27301 tgtcccccag ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat
    27361 cactttagct tgtgatgaaa ccagctcggt cagatccgtc ggaacaaact ctgcgcccat
    27421 tttttccagc aatttaccca ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt
    27481 ctggcataaa aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac
    27541 cttcatatca atccactgtt gttgagaaaa tgacgtgcgc attcttccgt gatttccccc
    27601 atgatgcaat gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt
    27661 tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc
    27721 atcaataacc agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg
    27781 atagtacgat agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct
    27841 ttcatgccaa ataacaacgt aagttgcttt ttcggttcaa cgacaatcac tttccagcta
    27901 tccaccgcat cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagattatga
    27961 ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc
    28021 tctttaccgc cgatttggtt caccaccagc cataaagccg caaggctggc ggacgtttta
    28081 acggtaaacc ccgcctgttt ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc
    28141 tgagcgtcgt agccccagtc gctggagttg accagttttt cttcctcttt caacgtgcta
    28201 cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca
    28261 tctgccagca gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg
    28321 gaagtaatca cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc
    28381 aaccagcggc gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc
    28441 acctctggtc cggcggcttc gaagatgcgg tgttcgttgg ctggatggtc taacagcgcc
    28501 accagatagt gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt
    28561 ggcggcgtta acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa
    28621 cctgcgccaa cgataattcc ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga
    28681 atgtccgccg tagcctgacg agctcgcaga tgatccgact gctcatgtgg cggggcctgc
    28741 aacgaactga gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg
    28801 agagccacct ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga
    28861 aaatagacgg tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg
    28921 actttatggc aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc
    28981 gccgccagga tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag
    29041 ccactggcac cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa
    29101 tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac
    29161 caggcggaca atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa
    29221 cgcggcagca ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac
    29281 atccgcgcct gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg
    29341 caaccgtgcg acgttatttt tcagggcata catcccggcg gcagccagaa tcccggactg
    29401 acgcatcccg ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt
    29461 accgacgagt aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga
    29521 atcacaatat tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat
    29581 gtgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt
    29641 caggtattca cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt
    29701 gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt
    29761 gccgtcggca gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc
    29821 ggcttcaaac agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca
    29881 gtgactgagc agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc
    29941 tttaccggaa agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta
    30001 aacgtcgtcc ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt
    30061 aacggtatca ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc
    30121 tttttacctt agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat
    30181 aattgcgcgc aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg
    30241 aatggctaat tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa
    30301 ggcgcgttgc agggcttcat caacttcaga ggctttttct acacggatac ccgtaatgcc
    30361 gcacgcttcg gcaatgcggg caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta
    30421 acctccggcc ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat
    30481 tttcaccggc agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc
    30541 gccatcgccg cacatggcga ccacctgacg ttccggctcg gttgcctgcg cacccaacgc
    30601 ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc
    30661 gttcattttc agataacgtg ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat
    30721 ggcgtcatcg gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt
    30781 ctcgctcggt ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagcgc
    30841 tttatccaga aacttgcgat cggttttttc ttccaccagt ggcagcaatg cgcgcagggt
    30901 tgatttgata tcgccgacca gcgccatatc taccttgcta tgcgcgccga tgctggctgg
    30961 attaatatca atctgaataa ttttggcatc ggtcgggtag aacgcacggt aggggaactg
    31021 agtaccgagt agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaatcc
    31081 gattaacccg gtcattccaa catcatacgg attatcgtat tcgacatgct ctttaccgcg
    31141 tagggcatga acaataggtg ctttaatttt cccggcaaac tcaactaact ctttatgcgc
    31201 ccccgcgcag ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc
    31261 cagtttgcgt aactcttctt cttccggcgt cacgactggc tgtggcgcat gataccagtg
    31321 cgtggttgcc ccttctggcg caggttttaa cgccacgtcg cccggtaaca caacaacgga
    31381 aacgccacgg ttaagcaccg ctttgcgcat agcaattgcc agcacttgtg ggatctgctc
    31441 cgggctggaa accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt
    31501 ttcctggaaa tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac
    31561 cggaacgtta ttgcggtggc aatcgaatag gccgttgatc aagtgcaggt tgcctgggcc
    31621 gcacgatccg gcacagaccg ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa
    31681 cgccgccact tcttcatggc gggtggacat ccactcgatg gtgcccatgc gattaagact
    31741 gtcgctaaga ccgttcagag agtcgcccgt gactccccag atgcgtttca cgcctgccga
    31801 ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa
    31861 tgtgataacg gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg
    31921 aggcactaac ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc
    31981 gagaaccaaa tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc
    32041 ggcgtcggtc agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt
    32101 acagcagccg caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag
    32161 cgcctccagt agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt
    32221 gaatttcaga ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa
    32281 acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc
    32341 gcaggtcatc acggtacgtg aagctaagtc aggtacatct gccagcagtt cgcgagtgag
    32401 acgaccagcg ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt
    32461 acgccactca tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac
    32521 atcggcctgt ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc
    32581 gcagcctgcc gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc
    32641 catcgcgtcc gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga
    32701 gccgacaccg tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt
    32761 ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata
    32821 ttgcccggcg cgatatgggt agtgatcgtg gcaaatcagg gaaatcgtcc acacatccgg
    32881 cgtttcttgc gtaatgtgat gaacctgcat ccgccacggg cattgattcg ttggcatcgt
    32941 catcgacaaa ctccttacgc gctcaacagt tgcttcatgt cttcttcaac agtggtgata
    33001 gaacgcaggc cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaactaggt
    33061 gcagtcgggc cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgaca
    33121 atcgctttct gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag
    33181 cccagtttct ctgccagagt gacagccaga ataattgctg agtaagcatc gttacattga
    33241 cctgcatcta ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga
    33301 tatttaccac aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg
    33361 gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc
    33421 agtttttcac ggctaaccag atcaatcagc gtatcggctg caccaagcag cgtctggcga
    33481 ccaaagccca cggtgatcag gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc
    33541 gcctgggcga tgaccgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca
    33601 acgatgctgc gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag
    33661 ttcgaggtca tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag
    33721 ccgctgccgt agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca
    33781 ggcagcattt cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg
    33841 ttgtagagat ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg
    33901 acgttgacct gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg
    33961 ctcatcactt tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata
    34021 tcggcaggcc aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg
    34081 tcgtattgac cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac
    34141 aggcacagca gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg
    34201 gtaaattctg ctgcctgacg ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca
    34261 gccatcgggt tatcgacgcg cgcgttggca tctacagcca ggcattgtgc tttcagcgcc
    34321 tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg
    34381 ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg
    34441 ccgtattcac gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa
    34501 tcctgaaggt cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg
    34561 tttcctgccg gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt
    34621 aaagttatat ttaatataca tgtttaaggt taagacgctt aacgcgggga taaaagggat
    34681 ttttcatgca actttaaggg agattgattt agcgcaattt tggcggcagg gatctaccgc
    34741 cagagaggta ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa
    34801 aaccgtgaac aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca
    34861 gggtgaaatc cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa
    34921 tcagcccagg gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg
    34981 cgctcccgat taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc
    35041 cgaaaccgga ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa
    35101 ttaaaccacc aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat
    35161 taaggacaat ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa
    35221 gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac
    35281 tcagaccaat ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca
    35341 tcgcaatacg cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct
    35401 ccagccacat cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat
    35461 aatgcagaat cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca
    35521 taaaaaagag aataaggtaa accatccagc ttaatagctg attaataact tttaacgcag
    35581 cttgttggcg aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc
    35641 cagaaaacat gaaaatccag tccttgcaaa gaagaagtcg aaatgcgcga tgacacacta
    35701 ctgaaagcgg aaggacgagt aaagttgcaa ttaaaaggaa atgttatgca tcagaggggg
    35761 atttttaatc ctatctgagt gtttagcatt gttgcgctcc tttcatgtgc aatgccatat
    35821 aaagccactg gcggagggag cgcgcagggg gcagccagtc gccgccgccc cctgcacccc
    35881 cgggctctgg cgagcaaaat cgccgctgcg cggtaccttc agcttatccc cttcggcaac
    35941 caggtcgggc gcgaggtaac gtcctgtaag acgcgccctc agcccacatc catgtgggct
    36001 gccccggcct tcggggaacg cttcagcgat tttgacgcca ccaatacccg tgcggtttgt
    36061 aatgtcgaag ataaaacaac cagggcagat aaaatgccaa tgctaattaa tcacgctttt
    36121 ccagcagagt ccggtaaatc agaccaccga taatgccgcc gacaattggc accacccaga
    36181 agaaccacag ttgttctaat gcccagccgc cctggaagat agcaaccgcg gtgctgcgcg
    36241 ccgggttaac agaagtgtta gtcaccggaa tactgattaa gtgaatcagg gttaaggcca
    36301 gaccaatagc gatcggcgca aaacctgccg gagcgaattt gtcggttgca ccgtggatca
    36361 ctaacaggaa gcccgcactc aataccagtt caactaccag cgcggaaagc atggaataac
    36421 cgcctggtga atgctcgcca taaccgttag aagcaaaacc gctggctgcc gcgtcaaaac
    36481 ccgttttacc actggcaatt aaatacagca gcgccgctgc aacaataccg ccgacaacct
    36541 gggcaatcac gtagccaacg acttcttttg ccggaaaacg tccgccagcc cataaaccaa
    36601 tagtgaccgc cgggttaaaa tgaccaccag aaatatgacc aacagcgaag gccatcgtca
    36661 gaacggtcaa accaaatgcc agcgccacgc cggcaaaacc aatgcctaat tccgggaagc
    36721 ctgcggccag tacagcacta ccacagccac caaaaacaag ccagaaagta ccaaaacatt
    36781 cagctgctaa ttttctgaac atatccacct caattaaaaa ttgaccctgt gaaaaatatg
    36841 gtcgttttat agggtcgtcg taaaaaatga cgacggaaat aatgcgcggc tattttaaaa
    36901 acgaaggcga gtcattcacc agataaataa atccagtaaa tttgatttag ggcaacagcg
    36961 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcaagcaaaa gatttaatcc
    37021 tttaggcgta ataaaaaata atttatcatg ctaattattt gattttgttg tttttgcggg
    37081 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca attgccatgc
    37141 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
    37201 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
    37261 gcggtatcaa ccgtttgtcg ttgatgctcg aacaaaacaa cgtcctgatt ggggagaacg
    37321 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
    37381 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
    37441 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
    37501 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
    37561 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
    37621 ataaagacgg acatccgatt gatgttgagg atattaacga tcaggcacgc catctggtgc
    37681 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
    37741 cggtgccaaa tgtccccaat gtggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
    37801 agttgtcctc acatccgcaa aatctctctg atggacagat tcgtcaggga ctttccgcaa
    37861 tggtgcagtt gcttgagcat tatttctctg agcagggggc tggacaggcg cgatatcgtt
    37921 taatgcggcg gcgggccagc aatgagcaac gaagctggcg ctatctggac atcatcaacc
    37981 ggatgattga ccgacctgga gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
    38041 ctttattgca ggcaaaaggc acattgcgac tggataaaga cgcgcgtccg ttattgctaa
    38101 ttgaagatcc agaaacccgc ctgcatccga ttatgctttc cgttgcctgg catctgttga
    38161 atcttttgcc tttacagcgt attgctacca ccaactctgg cgagctgctt tcgttaactc
    38221 cagtggaaca tgtttgccgc ctggtgcgtg aatcttctcg tgttgccgcc tggcgtctgg
    38281 ggccaagtgg cttgagcacc gaagacagcc gccgcatctc ttttcacatt cgttttaatc
    38341 gtccgtcatc gctgtttgct cgctgctggt tgctggtgga aggggaaacg gaaacctggg
    38401 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
    38461 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
    38521 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
    38581 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
    38641 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
    38701 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
    38761 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
    38821 taccaacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
    38881 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
    38941 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
    39001 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgactcc cagaacgcgt
    39061 tgtaatcagc atggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
    39121 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
    39181 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
    39241 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
    39301 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgga
    39361 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
    39421 actgctcacc gtttttgcct tccagtttag ccagttgcag cccgggggta ttcaaatata
    39481 agctgaattc ttccgccgac atacacccgc ggagtaacgc ataatggtaa cgtaacgcct
    39541 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
    39601 gtcgcggctg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
    39661 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
    39721 agcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
    39781 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
    39841 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
    39901 tgtgattact aacgtaaatt ttaggatttt gttgatattt cgttgaagtt aatgacccgg
    39961 attggcatgt ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
    40021 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
    40081 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
    40141 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgta ggcgcgcagg tcagcggtca
    40201 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
    40261 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
    40321 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
    40381 tcagcaacgt ctggcacaaa cgcaagcggt ttcactgcag gatctcgata ccgccgcgac
    40441 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
    40501 ggcctctctc gatacggcta aaaccaatct cgattacacc cgtatcgttg ccccgatggg
    40561 cggggaagtg acgcaaatca ccactctgca aggccagacg gtgattgccg cacagcaagc
    40621 accgaacatt ctgacgctgg cagatatgag cactatgctg gtaaaagcgc aggtttctga
    40681 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtgc ttggcgatcc
    40741 actgacgcgc tacgaggggc aaatcaagga tgtgctaccg acgccggaaa aggtaaacga
    40801 cgctattttc tattacgccc gttttgaagt acccaacccc aatggtttgc tgcggctgga
    40861 tatgaccgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgttga cgatccctct
    40921 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
    40981 tgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
    41041 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
    41101 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg tgatgagcag
    41161 gttgaggtgc tgaagggcat cagcctcaat atttatgcgg gtgagatggt cgcgattgtt
    41221 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
    41281 accagcggca cctatcgcgt agccggtcag gatattgcca cgctggacgc cgatgcgctg
    41341 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
    41401 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ccggtcttga gcggaaacag
    41461 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
    41521 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
    41581 aacggcggtc aggtaattct tgccgatgaa ccgaccggcg cactggacag ccattctggc
    41641 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
    41701 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
    41761 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
    41821 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
    41881 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
    41941 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
    42001 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
    42061 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
    42121 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
    42181 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
    42241 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
    42301 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
    42361 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
    42421 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
    42481 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
    42541 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
    42601 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
    42661 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggcgg tattggtgta
    42721 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
    42781 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
    42841 ctggtcggtg gcgcgttggg aataacactg tcactgttaa ttgctttcac cttgcagctt
    42901 ttcttacccg gctgggagat tggtttttca ccgttggcgc tgctgctggc gtttctctgc
    42961 tcgacggcca ccgggatttt atttggctgg ttacccgcac gaaatgcggc acgactggat
    43021 ccagtagatg ctctggcacg ggagtaattt ttgagataaa aatgccagcc gatcgggctg
    43081 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
    43141 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
    43201 aacggattgt ccagctttta gcgttctgta accatccatc tgaatggtgg aataatgagc
    43261 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaacctttgg cattgttgaa
    43321 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
    43381 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
    43441 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
    43501 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
    43561 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
    43621 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
    43681 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
    43741 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
    43801 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
    43861 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
    43921 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
    43981 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
    44041 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
    44101 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
    44161 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
    44221 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
    44281 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
    44341 tctggtcgct atcttcagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
    44401 agtcagccgt ctcgatgtgg tgaactttat ctctcatggc acgcgtaaag acgagccgac
    44461 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
    44521 tatggagaat ttcacgacga acctgaacca gcttgcgcgt gtgggcggaa tcgacccact
    44581 aattggtcgt gagaaagagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
    44641 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcag aaggtcttgc
    44701 ctggcgaatt gttcagggtg atgtgccgga agtgatggct gactgtacga tttactctct
    44761 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
    44821 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
    44881 caccattatc ggtgcgggtg cagcgtctgg tggtcaggtc gatgcggcta acctaatcaa
    44941 accgttgctc tccagcggta aaattcgcgt aattggttcg acaacctatc aggagttcag
    45001 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
    45061 accgtcgatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
    45121 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
    45181 cattaacgat cgtcatctgc cggataaagc cattgatgtt atcgacgaag cgggcgctcg
    45241 cgcacgtctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
    45301 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
    45361 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggccaggata aagccattga
    45421 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
    45481 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
    45541 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
    45601 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
    45661 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
    45721 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
    45781 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ctaacgccgg
    45841 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
    45901 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
    45961 ctggttcgat catctgtcaa ctgacgtgat ccatcaggtg gtggataaat tcatcgtcga
    46021 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
    46081 ctggctggcc gagaaaggtt acgaccgggc aatgggcgca cgtccgatgg cgcgtgtcat
    46141 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
    46201 cggtcaggtg acggttgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
    46261 tgcacaaaag cacaaggcgg aagcagcgca ttaatctgat tgtcaggtag gttggtgaag
    46321 tccgtaatct cgtcaggggt tacggacttt ttatttatgg ggtggaacgg tgaaaaccct
    46381 atttttggag gtgaaggtaa gttgttgata attagtgctg ctggaaggta aggataaaaa
    46441 agggtgctgc aggagaatgg gatggttttg ctttattcac aacgggctaa acgtgtagta
    46501 tttgagttca ctgccgtaca ggcagcttag aaatcgacgg ggtgcggtaa aacctttgcg
    46561 aacgcgttca ctgccgtaca ggcagcttag aaattcacag gtaacatact ccacccgccc
    46621 accatgttca ctgccgtaca gacagataaa atgcgaaaaa aaagctcgca ctttcgtacg
    46681 agctcttctt taaatatggc ggtgaggggg ggattcgaac ccccgatacg ttgccgtata
    46741 cacactttcc aggcgtgctc cttcagccac tcggacacct caccaaattg ttttgctacc
    46801 aaacctcatg ggtggcaacg gggcgctact atagggagtt ggagtaaaac ggtcaagaag
    46861 aattttaatg ataattattg tttgctcata ctgtaaacaa cttgtgcagt atatctacat
    46921 cgagacaagt tatggactta tacttccaaa gtacttcata catatcacaa aataaaaagg
    46981 ccggttaaac cgacctttta ctcgttcttt ctcttcgccc atcaggcggt aaaacaatca
    47041 gcgactacgg aagacaatgc ggcctttgct caggtcgtac ggggtcagtt caacagtcac
    47101 tttgtcgccc gtcaggatgc ggatgtagtt tttgcgcatt ttaccggaga tgtgtgcagt
    47161 aaccacgtga ccgttttcta actctacgcg gaacatggta ttaggcaacg tttcaagaac
    47221 ggtaccttgc atttcaatat tgtcttcttt ggccatctaa tcctctgggg tatcactacc
    47281 gtaatttgaa ccggcaagat aatgccgaag ttctgtaaat aagtaaagat ttgcgcgcta
    47341 aatcgcaaca aacaggttcg gcacattact ccgaaaacac acggctaagc cgcaccaaaa
    47401 gcgcaacgta taagggagcg gtgagataaa cgatgggcgt tacctgacgc gaaaaattcc
    47461 ttatcggcag cggggtaatg agcgtaacca actctgcgac cgcaattata acactctggg
    47521 gagaaatgtg ccgaaaacat tcattcttgt ggtgaaaaca agcaccgtgg tacccagaaa
    47581 ttattcggca atcgtccgag gcgcatttga ttgagataat taaggtaatc ccggcgggga
    47641 atttcgcagg caccaagcga tgctgtgtga tcgttaagga cctggcagtc gataagctta
    47701 ccgccatgac cgataaattc ctcacagcat accagaagcg ccgttttaga cgcattttcc
    47761 atccggctga acatggactc gccacaaaat agcgttccct gggccacgcc gtacataccg
    47821 ccgacaagct catcttcacg ccagacttca atggagtggg catgaccgag ttcgtgaagg
    47881 cgatggtagg cttcgaccac gccacgcgtg atccaggttc cttcttcgcg atcgctggca
    47941 cagccttcaa tgacctgacc aaaagcgtaa ttcatcgtga cacgataggg cgagcgttta
    48001 tgaaatcgct tcatactacg gctgatatgc agtgattctg gccatagcac cgcgcgggga
    48061 tcgggcgacc accagaggat ggggtcgcct ggagaaaacc acggaaaaat accacgctgg
    48121 taagccatta acaggcgcgc agggctaaga tcgcccccaa gtgccagcag gccgttaggc
    48181 tcacgtaatg cgccttccgg ggaagggaag gctattgaat ggcgagaaag ctgaaccagg
    48241 cgcatgaccg caaaactcca cgcaagtcgg atcgttcaat aatagcttac aaaccctgct
    48301 tgaactggta ataacgcccc tgtctggcaa gcagttctgc gtgagtacct tgctcaataa
    48361 tttgcccgtt gtccatcact attatttgtt ggaaacgaga gagtccgcga agtcgatggg
    48421 tgaccattaa caccgttttc tcacgcatca tttttgcaag caattcaagg atctggcttt
    48481 cggttgtggc atctaagcct tcggtaggtt catccagcaa caccagtggc gcatcatgta
    48541 acagcgcacg ggcgatagcc agacggcgca gttcaccacc ggagagctgg cgtccgcctt
    48601 cacctaacca actgttgaga cctgcatcct cgagcagctt ctccagacca acgcgacgca
    48661 agatctccga cagagcctca tcactactgc caggcgaggc gagtaacaga ttatcacgca
    48721 gcgtggcgct aaacagatgc actcgctgag gaacaacgct gatggtctgt cgtagagccg
    48781 cttcattcag gctggctatg gggctatcgt taagcaaaat ctcgccctgt tgcgggtccc
    48841 atgcgcgggt cagcagttgt aacagtgttg atttgccgca tccggttcgc ccgagaatcg
    48901 ctatatgttc cccggcgttt acctgaagag aaatcccttt aagtgcctgt tgggattgct
    48961 ccggataagt gaactgaaca tcccgtaatg tcagcgaaac gcgatcggca acacgagttt
    49021 gggtatcagg gaaggtgacc tccggttttt gatccgttaa gtcagagata cgtacggcag
    49081 aggcaatgac ttgccccaga tgctgaaatg cacccgttac tggtgccagt gcttcaaacg
    49141 cggctaacgc acaaaagaca aacagggcaa ttaatgcgcc gggttgagca ttgccgccaa
    49201 cgccgccaga cgccatccac agcatcagga tcaccgctaa cgcgccaatg agcagcatta
    49261 tcgcttgcga caatgcggtc agttcagatt gacggcgttg cgcttccagc cattgaattt
    49321 ctgtattctc tagttgcgtg cgataacgat cgctggcacc aaaaatggtc agctcagctt
    49381 gcccttgcag ccaggccgtc agttgttggc gatactgtcc gcgaagatga gtcagatttt
    49441 gcccggtgct ttttcccgca cgataaaaca gcggtggcat caggaaaagc gtcagtaaca
    49501 taacgctgcc cagcgtaaag gcgagggtga aatcaaggaa acttaacccg attgtcacca
    49561 ccataatcac cacaaaagcg cccaccagcg gcgagataac gcgcaggtaa agatgatcga
    49621 gcgtatcaac atccgccacc acgcggttga gcaattcgcc ctgacgatag cgcgccagtc
    49681 cggcagggga gaggggcaac aatttgctga aggtgtaaat gcgcagatgc tgcaacacgc
    49741 ggaaagtcgc gtcgtgactt accagacgtt caaaataacg cccggcagta cgggtgattg
    49801 cggcaccacg cacgcccgca gcgggtagca tatagttgaa gctgtacagt ccggcaaccc
    49861 ccgcaaccgc tgaggccgag aggaaccagc cggaaagtgt caacagaccg atactggcga
    49921 gcagcgtcac aattgccagc acaataccaa gacttaacat ccatttatga cgtttataca
    49981 gtgccagata gggtagcaaa gcgcgcattt aaatctcctc ctgacgatgg gccagtaatg
    50041 tggcgaatgg gccaccagcc acacttaatt ccgcgtaacg tccttgctca ataatccggc
    50101 catcctgcat aacccaaatg acatcccagt cagcaagatc ttctaactgg tgggtgacca
    50161 ttaacgttgt ctggcgcaga gaggcggcat tcagcgcctc cattacgcgc tgttcactgt
    50221 gagcatcaag gctggcagcg ggttcatcca acagtaatag cgaacaggga tttagtaacg
    50281 cacgggccac cgccacgcgc tgcgcctgcc ccacggaaag gcgggcagcc tggtcgccaa
    50341 caggtgtatc aacgccttgt ggcaggagcg gtagaaactc gctgacccag gcgttatcca
    50401 gcgctgcttg taattcttgt tcgctggcat caggtcgcgc cagtagtacg ttatcccgca
    50461 attttgctgc cggtaattgt gggttttgcc caacccagga gagatgttta cgccatgatt
    50521 ctggtgataa atcacgtaat tctatcccgt tgattcgtaa tgatccctga tatgagagaa
    50581 aaccagaaag cgcgttcagc agtgagcttt tacctgaacc gctgcggcca accaacactg
    50641 cacgttggcc tgctggcaaa gtaaagttca gcggtccggc cagcgtttta ccttccggcg
    50701 acgtgataaa cagatcctcg gcctcaatgg tcagcggatc ggtcaatgct aattccgcct
    50761 caccgcgctg cggatgggcg agcggggttt ccataaacgt tttcagactg tcagctgcac
    50821 caacagcctg ggctttagca tgataaaacg taccgagatc gcgtaatggc tggaaaaact
    50881 ctggcgcaag gatcagggcc agaaaacctg cagccagcgt caccccagta tcgtagtgac
    50941 caaaatccag ctcgccgaga taggaaaaac caaagtagac cgccaccaga gcaattgaca
    51001 gcgaggtaaa aaattcgaga atgccggagg ataaaaacgc cagtcgtagc acttccattg
    51061 tccgttggcg gaaatcttcc gaagcagaac gaatactttc aatttcagct tcaccacgac
    51121 caaaaatacg caatgtttcc atgccgcgca ggcgatcgag gaaatgccca cttaagcgag
    51181 caagagcgag aaagttacgc cggttagcat cggcagcccc cattccaacc agcgccataa
    51241 acaacggaat tagcggtgct gtgcccagca gaatgagcgc cgcagcccag ttagagggga
    51301 agatcgccac cacaatcagc aacggcaccg acactgccag cgccatttgc ggcagatagc
    51361 gtgcatagta atcatgcata tcgtcaattt gctcgagtac cagcgtcgcc cagctccccg
    51421 caggtttacc ctgaatccac gctggccctg cttgttgcag acggtcgaga acctgacggc
    51481 ggatggcaaa gcggatatgc tgcccggcgt gataacccac ccgttcgcgt aaccagacca
    51541 cccatgcgcg caatacaaag gtcagaacca gtaacgtaaa gggaagcagc agggcttcac
    51601 ggggaatatt ctccataatc atatgttgca gaattcgcgc catgaaccag gcctgggcaa
    51661 tgatcaatat gccgcttaca aagcccagca gacgagaaat attcagccaa cgttgggaga
    51721 tgacgctttg ctgttttaac cagcgggtta actctttttg acgagattta ttcattgcac
    51781 gcttagcagg tgagttatca gaattatttg cagagcaatg ttacaacggg gaaaaaataa
    51841 aggcgaccca tagtcgcaag gtgtcgcctt ctttactttt gttactgatt tgtaaaatta
    51901 ttttgcgtca gctaaaccat cgaggtagcg ttccgcatca agtgctgcca tgcagccagt
    51961 accggccgaa gtaatggcct ggcgataaat gtgatccatc acgtcgcctg cggcaaagac
    52021 gccaggaatg ctggtctggg tggcattacc atgaataccc gactgtactt tgatgtagcc
    52081 gttttccagt tccagctgcc cttcgaaaat cgcagtattc gggctgtgac cgatagcaac
    52141 aaacagaccg gcaacgtcga gtgactcgat gttatcgctg ttttgcgtat cgcgcagacg
    52201 aacgccagtg acacccattt gatcgccggt cacttcttcc agcgtacggt tggtgtgcag
    52261 aatgatgttg ccgttctcca ctttatccat cagacgctta ataaggattt tttccgcgcg
    52321 gaaaccgtca cggcggtgaa tcagatgcac ttccgaagcg atgttagaca gatacagcgc
    52381 ctcttcaacc gcggtattgc cgccgccgat gaccgcaact ttctggttgc gatagaagaa
    52441 accgtcgcag gttgcacaag cagaaacccc acggccttta aaggcttctt cagagggcag
    52501 gccgagatag cgtgcagaag ctccggtggc aataatcagc gcgtcgcaag tgtattcgcc
    52561 gttatcgcca ttcagacgga acggacggtt ttgcagatcc accttgttga tatgatcaaa
    52621 aatgatctca gtttcaaact tggtggcatg ttcgtgcatg cgcgccatta ataacggacc
    52681 ggtcagatcg tttggatcac caggccagtt ttccacttcc gtggtggtgg tcagttggcc
    52741 gcctttttcc atgccggtaa tcagcacagg ttgcaggttg gcgcgcgccg cgtagacagc
    52801 agcggtgtat cccgccgggc ctgaacccag gataagcagt ttactgtgtt tggtcgtgcc
    52861 catgagatcc ccatagttgt tggcagacaa tgggcaggat tgtagggaat ttacagacgt
    52921 aaaaaaagag tatgacgatt ttgttaacaa tttgtgcaat cggcagcatc gataagcagg
    52981 tcaaattctc ccgtcattat cacctctgct acttaaattt cccgctttat aagccgatta
    53041 aatgatgaat aaacgcccct gttaatgaat atctggcatg ttgtactaaa aatcgatgtt
    53101 ttgctttgac aatcccctgg tgttttgcga aaacattcga ggaagaaaaa aaacagtatt
    53161 cttatatgcg cataaccatg tatgtaaata ccatgtttac cgtgctagtg aaatctacgt
    53221 atggcgtgga cagacgccat tcgtgatgtc gatagctgcc acaaggcaac ggtcttctca
    53281 ccgtagaccc aggcattgcg cgccgtgaat cttcatgatt tcggtctatc gtgacgggta
    53341 gcgactctga acagtgatgt ttcagggtca gacaggagta gggaaggaat acagagagac
    53401 aataataatg gtagatagca agaagcgccc tggcaaagat ctcgaccgta tcgatcgtaa
    53461 cattcttaat gagttgcaaa aggatgggcg tatttctaac gtcgagcttt ctaaacgtgt
    53521 gggactttcc ccaacgccgt gccttgagcg tgtgcgtcgg ctggaaagac aagggtttat
    53581 tcagggctat acggcgctgc ttaaccccca ttatctggat gcatcacttc tggtattcgt
    53641 tgagattact ctgaatcgtg gcgcaccgga tgtgtttgaa caattcaata ccgctgtaca
    53701 aaaacttgaa gaaattcagg agtgtcattt agtatctggt gatttcgact acctgttgaa
    53761 aacacgcgtg ccggatatgt cagcttaccg taagttgctg ggggaaaccc tgctgcgtct
    53821 gcctggcgtc aatgacacac ggacatacgt tgttatggaa gaagtcaagc agagtaatcg
    53881 tctggttatt aagacgcgct aacacggaac aggtgcaaaa tcggcgtatt ttgattacac
    53941 tcctgttaat ccatacagca acagtactgg ggtaacctgg tactgttgtc cgttttagca
    54001 tcgggcagga aaagcctgta acctggagag cctttcttga gccaggaata cactgaagac
    54061 aaagaagtca cattgacaaa gttaagtagc ggccgccgcc ttctggaggc gttgctgatc
    54121 cttattgtcc tgtttgccgt ctggttgatg gctgccttac taagctttaa cccttcggac
    54181 cccagctggt cgcaaacggc ctggcatgaa cctatccata atttaggtgg gatgcccggt
    54241 gcgtggttgg cagatacgct gttctttatt tttggcgtga tggcttacac cattcccgtc
    54301 attattgtcg gcggttgttg gtttgcctgg cgtcatcagt ctagcgacga atacattgat
    54361 tattttgccg tttcgctacg catcattggc gttttggcgc tcatccttac ctcctgtggt
    54421 ctggcggcaa tcaacgctga cgatatctgg tattttgcct ccggtggcgt cattggcagc
    54481 ttactaagca ctacgctaca accactgcta cacagtagcg ggggaactat tgcgctgctc
    54541 tgcgtttggg cagcgggcct gacgttgttc accggttggt catgggtgac cattgctgaa
    54601 aaactcggcg gctggatttt aaacattctc accttcgcca gtaatcgtac ccgtcgcgat
    54661 gatacctggg tcgatgaaga tgagtatgaa gacgacgaag agtatgaaga tgaaaatcac
    54721 ggcaaacagc atgaatcacg ccgtgcccgt attcttcgcg gcgcgctagc gcgtcgtaaa
    54781 cggttggcgg aaaaattcat taatccgatg gggcggcaaa cagacgctgc gttgttctcc
    54841 ggtaagcgga tggatgatga cgaagagatt acctacactg cacgcggtgt ggctgtcgac
    54901 ccggacgacg tcctattttt gggcaatcgt gcaacgcagc cagaatatga cgaatacgat
    54961 ccattattaa acggtgcgcc aattaccgaa cctgtcgctg tagcagctgc tgctaccacg
    55021 gcgacacaaa gctgggctgc gccggttgaa cctgtgactc agacgccgcc tgttgcctct
    55081 gttgatgttc caccttcgca acctacagta gcctggcagc ctgtaccggg tccacaaacg
    55141 ggagagccgg ttattgctcc tgcaccggaa ggttacccac agcagtcaca atatgcgcag
    55201 cctgcagtgc aatataatga gccgctgcaa caaccagtac agccgcagca gccgtattat
    55261 gcacctgcag ctgaacaacc tgcgcaacaa ccttactacg ctcctgcggc ggaacaacct
    55321 gttcagcaac cgtattacgc aactgcgccg gaacagcctg cgcaacagcc gtattatgcc
    55381 cctgcgccag aacaaccggt ggcaggtaac gcctggcaag ccgaagagca gcaatccatt
    55441 tttgctccac agtctacata ccagactgag caaacttatc agcagccagc cgctcaggag
    55501 ccgttgtacc aacagccgca acccgttgaa cagcagcctg ttgtggagcc tgaacccgtt
    55561 gtagaagaga caaaacccgc gcgtccgccg ctttactatt ttgaagaagt ggaagagaag
    55621 cgagcccgtg aacgtgaaca acttgcggcc tggtatcaac cgattccaga accggttaaa
    55681 gaaccagaac cgatcaaatc ttcgctgaaa gcaccttctg ttgcagcagt acctccagta
    55741 gaagccgctg ccgctgtttc tccgctggca tctggcgtga aaaaagcgac actggcgacg
    55801 ggggctgccg caaccgttgc cgcgccagtc ttcagtctgg caaatagcgg tggaccgcgt
    55861 ccgcaggtca aagaggggat tggcccgcag ttgccacgac cgaaacgtat ccgcgtgcca
    55921 actcgtcgtg aattggcgtc ttacggtatt aagctgccct cacagcgtgc ggcggaagaa
    55981 aaagcccgtg aagcccagcg caatcagtat gattctggcg atcagtataa cgatgatgaa
    56041 atcgatgcga tgcagcagga tgaactggca cgtcagttcg cccagacaca gcagcaacgc
    56101 tatggcgaac agtatcaaca tgatgtgccc gtaaacgcag aagatgcaga tgctgcggca
    56161 gaggctgaac tggctcgtca gtttacgcaa actcaacaac aacgttattc cggcgaacaa
    56221 ccggctgggg cgaatccgtt ctcgctggat gattttgaat tttcgccaat gaaagcgttg
    56281 ctggatgatg gtccacacga accgctgttt acgccaattg ttgaacctgt acagcagccg
    56341 caacaaccgg ttgcaccaca gcagcagtat cagcagccac aacagccggt tgcgccacag
    56401 ccgcagtatc agcagccaca acagcaggtt gcgccgcagc cacaatatca gcagccgcaa
    56461 caaccggttg cgccacagca gcaatatcag cagccgcaac aaccagttgc gccgcagccg
    56521 cagtatcaac agccgcaaca accggttgca ccgcagccgc agtatcagca gccacaacag
    56581 ccagttgcgc cacagccgca ggataccctg cttcatccgc tgttgatgcg taatggcgac
    56641 agccgtccgt tgcacaaacc gacgacgccg ctgccttctc tggatttgct gacaccgccg
    56701 ccgagcgaag tggagccggt agataccttt gcgcttgaac aaatggcgcg tctggtggaa
    56761 gcgcgtctgg ctgatttccg tattaaagcc gatgtcgtta attactctcc ggggccagtt
    56821 atcactcgct ttgaacttaa cctggctcct ggcgtaaaag cggcgcgcat ttctaacttg
    56881 tcacgggacc ttgcccgttc actttcgacg gtggcggtgc gtgtcgttga agttattcct
    56941 ggcaaaccct atgtaggtct ggagttacca aataaaaaac gacaaaccgt ttatctgcgc
    57001 gaagttttgg ataacgccaa attccgcgat aatccgtcgc cattaaccgt ggtgctgggt
    57061 aaagatatcg ccggtgagcc ggtggttgcc gatctggcga aaatgccgca cttgttggtt
    57121 gcggggacta ccggttccgg taaatctgtc ggtgtgaacg cgatgatcct gagcatgctt
    57181 tataaagcac agccagaaga tgtgcgtttc atcatgatcg acccgaaaat gctggagctt
    57241 tcggtttatg aaggcattcc gcacctgtta acggaagtcg ttactgatat gaaagatgcc
    57301 gccaacgcgc tgcgctggtg tgttaacgag atggagcgtc ggtataaact gatgtctgcg
    57361 ctgggtgtgc gtaatctggc gggttataac gaaaaaattg ctgaagccga tcgcatgatg
    57421 cgtccgattc cagacccgta ctggaagccg ggtgacagta tggatgccca gcatccggtg
    57481 ctgaaaaaag aaccatacat tgtcgtgttg gttgacgaat ttgccgacct gatgatgacg
    57541 gtaggtaaaa aagtggaaga gctgatagca cgtctggcgc aaaaagcccg tgccgcgggt
    57601 atccacctcg tactggcaac tcagcgtcca tcggttgatg ttattactgg tctgattaaa
    57661 gcgaatattc cgacccgtat cgcctttacc gtatccagta agattgactc acgtaccatt
    57721 cttgatcagg ctggcgcgga atcactgctg ggtatggggg atatgctcta ctctgggccg
    57781 aactccacgt tgccggtacg tgtccatggt gcttttgttc gcgatcagga agttcatgcc
    57841 gtggtgcagg actggaaagc gcgtggtcgc ccacagtatg ttgatggcat cacctccgac
    57901 agcgaaagcg aaggtggtgc gggtggtttc gatggcgctg aagaactgga tccgttgttc
    57961 gatcaggcgg tgcagtttgt cactgaaaaa cgcaaagcgt caatttctgg cgtacagcgt
    58021 cagttccgca ttggttataa ccgtgcagcg cgtattatcg aacagatgga agcgcagggg
    58081 attgtcagcg aacaggggca caacggtaat cgtgaagtgc tggccccacc gccgtttgac
    58141 taattaatgc attgccggat aaggcgcggt agcgtcgcat ccggcactct atcaactgaa
    58201 aattcagtat tttcttcttt cctcaagctg attattagcc tggaatagag agtagaggga
    58261 actcccgatc gggagtgacg taatttgagg aataatgatg aaaaaaattg ctatcacctg
    58321 tgcattactc tcaagcttag tagcaagcag cgtttgggct gatgccgcaa gcgatctgaa
    58381 aagccgcctg gataaagtca gcagcttcca cgccagcttc acacaaaaag tgactgacgg
    58441 tagcggcgcg gcggtgcagg aaggtcaggg cgatctgtgg gtgaaacgtc caaacttatt
    58501 caactggcat atgacacaac ctgatgaaag cattctggtt tctgacggta aaacactgtg
    58561 gttctataac ccgttcgttg agcaagctac ggcaacctgg ctgaaagatg ccaccggtaa
    58621 tacgccgttt atgctgattg cccgcaacca gtccagcgac tggcagcagt acaatatcaa
    58681 acagaatggc gatgactttg tcctgacgcc gaaagccagc aatggcaatc tgaagcagtt
    58741 caccattaac gtgggacgtg atggcacaat ccatcagttt agcgcggtgg agcaggacga
    58801 gcagcgcagc agttatcagc tgaaatccca gcaaaatggg gctgtggatg cagcgaaatt
    58861 taccttcacc ccgccgcaag gcgtcacggt agatgatcaa cgtaagtaga ggcacctgag
    58921 tgagcaatct gtcgctcgat ttttcggata atacttttca acctctggcc gcgcgtatgc
    58981 ggccagaaaa tttagcacag tatatcggtc agcaacattt gctggctgcg gggaagccgt
    59041 tgccgcgcgc tatcgaagcc gggcatttgc attctatgat cctctggggg ccaccgggta
    59101 ccggcaaaac aaccctcgct gaagtgattg cccgctatgc gaacgctgat gtggaacgta
    59161 tttctgccgt cacctctggc gtgaaagaga ttcgcgaggc gatcgagcgc gcccggcaaa
    59221 accgcaatgc aggtcgccgc actattcttt ttgttgacga agttcaccgt ttcaacaaaa
    59281 gccagcagga tgcatttctg ccacatattg aagacggcac catcactttt attggcgcaa
    59341 ccactgaaaa cccgtcgttt gagcttaatt cggcactgct ttcccgtgcc cgtgtttatc
    59401 tgttgaaatc cctgagtaca gaggatattg agcaagtact aactcaggcg atggaagaca
    59461 aaacccgtgg ctatggtggt caggatattg ttctgccaga tgatacacga cgtgccattg
    59521 cagaactggt gaatggcgac gcgcgccggg cgttaaatac gctggaaatg atggcggata
    59581 tggccgaagt ctatgatagc ggtaagcggg tcctgaagcc tgaattactg accgaaatcg
    59641 ccggtgaacg tagcgcccgc tttgataaca aaggcgatcg cttttacgat ctaatttccg
    59701 cactgcataa gtcggtacgt ggtagcgcac ccgatgcggc gctgtactgg tatgcgcgaa
    59761 ttattaccgc tggtggcgat ccgttatatg tcgcgcgtcg ctgtctggcg attgcgtctg
    59821 aagacgtcgg taatgccgat ccacgggcga tgcaggtggc aattgcggcc tgggattgct
    59881 ttactcgcgt tggcccggcg gaaggtgaac gcgccattgc tcaggcgatt gtttacctgg
    59941 cctgcgcgcc aaaaagcaac gctgtctaca ctgcgtttaa agccgcgctg gccgatgctc
    60001 gcgaacgccc ggattatgac gtgccggttc atttgcgtaa tgcgccgacg aaattaatga
    60061 aggaaatggg ctacgggcag gaatatcgtt acgctcatga tgaagcaaac gcttatgctg
    60121 ccggtgaggt ttacttcccg ccggaaatag cacaaacacg ctattatttc ccgacaaaca
    60181 ggggccttga aggcaagatt ggcgaaaagc tcgcctggct ggctgaacag gatcaaaata
    60241 gccccataaa acgctaccgt taatgttatc gttgcggtaa tgttgttact gtatccctgt
    60301 ggtcgcaggc tgtggccaca tctcccattt aattcgataa gcacaggata agcatgctcg
    60361 atcccaatct gctgcgtaat gagccagacg cagtcgctga aaaactggca cgccggggct
    60421 ttaagctgga tgtagataag ctgggcgctc ttgaagagcg tcgtaaagta ttgcaggtca
    60481 aaacggaaaa cctgcaagcg gagcgtaact cccgatcgaa atccattggc caggcgaaag
    60541 cgcgcgggga agatatcgag cctttacgtc tggaagtgaa caaactgggc gaagagctgg
    60601 atgcagcaaa agccgagcta gatgctttac aggctgaaat tcgcgatatc gcgctgacca
    60661 tccctaacct gcctgcagat gaagtgccgg taggtaaaga cgaaaatgac aacgttgaag
    60721 tcagccgctg gggtaccccg cgtgagtttg actttgaagt tcgtgaccac gtgacgctgg
    60781 gtgaaatgca ctctggcctc gactttgcag ctgcagttaa gctgactggt tcccgctttg
    60841 tggtaatgaa agggcagatt gctcgcatgc accgcgcact gtcgcagttt atgctggatc
    60901 tgcataccga acagcatggc tacagtgaga actatgttcc gtacctggtt aaccaggaca
    60961 cgctgtacgg tacgggtcag ctgccgaaat ttgctggcga tctgttccat actcgtccgc
    61021 tggaagaaga agcagacacc agtaactatg cgctgatccc aacggcagaa gttccgctga
    61081 ctaacctggt gcgcggtgaa atcatcgatg aagatgatct gccaattaag atgaccgccc
    61141 acaccccatg cttccgttct gaagccggtt catatggtcg tgacacccgt ggtctgatcc
    61201 gtatgcacca gttcgacaaa gttgaaatgg tgcagatcgt gcgcccagaa gactcaatgg
    61261 cggcgctgga agagatgact ggtcatgcag aaaaagtcct gcagttgctg ggcctgccgt
    61321 accgtaaaat catcctttgc actggcgaca tgggctttgg cgcttgcaaa acttacgacc
    61381 tggaagtatg gatcccggca cagaacacct accgtgagat ctcttcctgc tccaacgttt
    61441 gggatttcca ggcacgtcgt atgcaggcac gttgccgcag caagtcggac aagaaaaccc
    61501 gtctggttca taccctgaac ggttctggtc tggctgttgg tcgtacgctg gttgcagtaa
    61561 tggaaaacta tcagcaggct gatggtcgta ttgaagtacc agaagttctg cgtccgtata
    61621 tgaacggact ggaatatatt ggctaatacc caatttttct gaatctaaaa agcgccttta
    61681 gggcgctttt tttgtctccc tttgataccg aacaataatt actcctcact tacacgtaat
    61741 actactttcg agtgaaaatc tacctatctc tttgattttc aaattattcg atgtatacaa
    61801 gcctatatag cgagctgcta tagaaataat tacacaatac ggtttgttac tggaatcaat
    61861 cgtgatcaag cttgagtgag ccattatgaa aacgaaaatc cctgatgcgg tattggctgc
    61921 tgaggagagt cgccgtggtt tggtaaaaac gacagcgatc ggcggcctgg caatggccag
    61981 cagcgcatta acattacctt ttagtcggat tgcgcacgct gtcgatagcg ccattccaac
    62041 aaaatcagac gaaaaggtta tctggagcgc ctgtacagtt aactgtggta gtcgctgccc
    62101 gctacgtatg cacgtcgtgg acggtgaaat caaatatgtc gaaacggaca ataccggcga
    62161 tgacaattac gacggcctgc actaggttcg cgcctgcctg cgtgggcgtt ccatgcgtcg
    62221 ccgtgtctac aatccggacc gcctgaaata tccgatgaaa cgagtcgggg cgcgcggtga
    62281 aggcaaattc gagcgcatta gctgggaaga agcctacgac atcatcgcga ccaatatgca
    62341 gcgcctgatc aaagagtacg gcaacgagtc tatctatctg aactatggca ccggtacgct
    62401 gggcggcacc atgacccgct cctggccgcc gggaaatacc ctggtcgcgc gcctgatgaa
    62461 ctgctgcggc ggttatctta accattacgg tgactactct tccgcgcaaa ttgcggaagg
    62521 tttgaaccat acctacggcg gctgggcaga tggtaacagc ccgtcggata tcgaaaacag
    62581 taaattggta gtgctgtttg gtaataaccc aggcgaaacg cgaatgagtg gcggtggggt
    62641 gacttactat cttgaacagg cacgccagaa atctaatgct cgcataatca tcatcgatcc
    62701 gcgctatacc gacaccggtg ccgggcgcga agatgagtgg atccctattc gtccaggaac
    62761 agatgccgca ctggttaacg gtctggcgta cgtcatgatc actgaaaacc tggtggatca
    62821 ggcattcctc gataaatatt gcgttggcta tgacgagaaa accctgcccg ccagtgcgcc
    62881 gaaaaatggt cattataaag cttatattct gggtgaaggg ccagatggcg tggctaaaac
    62941 accggaatag gcctcgcaaa tcaccggtgt tccggcagac aaaatcatca aactggctcg
    63001 tgaaatcggc agtaccaaac cggcgtttat cagccaggga tggggcccgc agcgtcacgc
    63061 taacggtgaa atcgcaaccc gtgctatctc gatgctggcg attctgaccg gtaacgttgg
    63121 tattaatgga gggaacagcg gcgcgcgtga aggttcatac agcttaccgt ttgtccgtat
    63181 gccgaccttg gaaaacccga tccagaccag catttcgatg tttatgtgga ccgatgccat
    63241 tgaacgtggc ccggaaatga cggcgctgcg tgatggtgtg cgcgggaaag ataagctgga
    63301 tgtgccgatc aaaatgatct ggaactatgc cggtaactgc ctgattaacc agcattctga
    63361 aatcaaccgt acccatgaaa tcctccagga tgataagaag tgcgagctga ttgtggttat
    63421 cgactgccac atgacctcat cggcgaaata tgctgacatc ctgctgcctg actgcaccgc
    63481 ttccgaacag atggactttg cactggatgc atcctgcggg aatatgtctt acgtgatttt
    63541 caacgatcag gtgattaaac cgcgctttga atgtaagacc atctatgaaa tgaccagcga
    63601 actggcaaaa cgtcttggcg ttgagcaaca gtttactgaa ggccgtaccc aggaagagtg
    63661 gatgcggcat ctgtatgccc agtcgcggga agcgattcct gaactgccaa cgtttgaaga
    63721 gttccgcaag caggggatct ttaaaaagcg cgacccacaa gggcatcacg ttgcttataa
    63781 agccttccgt gaagatccgc aggcaaaccc actgactacg ccatcgggca aaattgagat
    63841 ttattcgcag gcgctggctg acattgccgc tacctgggaa ttgccagaag gcgatgtgat
    63901 cgatccactg ccgatctaca cgccgggctt tgaaagttat caggatccgc tgaacaaaca
    63961 gtatccgctg cagcttacag gtttccacta taaatctcgc gttcactcaa cttacggcaa
    64021 cgttgatgtg ctgaaagcgg cttgccgtca ggaaatgtgg atcaacccgc ttgatgccca
    64081 aaaacgcggt atccacaacg gcgataaagt caggatcttt aacgatcgtg gtgaggttca
    64141 tattgaggcg aaagtgacgc cacgaatgat gccgggcgtg gtcgcactgg gtgaaggcgc
    64201 ctggtatgac ccggatgcaa aacgtgtcga taagggcggt tgtatcaacg tactgaccac
    64261 tcaacgtccg tctcctctcg ctaaggggaa tccgtcacat acaaaccttg ttcaggttga
    64321 aaaggtgtaa ggagtaaccg atgacaaccc agtatggatt ttttattgat tccagccgtt
    64381 gcaccggttg caaaacctgc gagctggctt gtaaagacta caaagatttg acgccagaag
    64441 tcagtttccg ccgcatttat gaatatgctg gcggcgactg gcaggaagat aacggtgtct
    64501 ggcaccagaa cgtgtttgcc tactatctgt cgatttcgtg caaccactgc gaagatccgg
    64561 cttgtactaa agtctgcccg agcggtgcga tgcataaacg cgaagatggt tttgttgtgg
    64621 tcgatgaaga tgtatgcatt ggctgccgtt actgtcatat ggcttgcccg tatggcgcac
    64681 cgcaatataa cgaaacgaaa ggccatatga ccaaatgcga tggttgttat gaccgtgttg
    64741 cggagggtaa aaagccgatc tgtgttgaat cttgcccgct gcgggcgctg gatttcggcc
    64801 ctatcgacga gctgcgtaaa aaacatggcg atctggcggc cgttgcgccg ttgccgcgag
    64861 ctcactttac caaaccgaat attgtgatca aacccaatgc caatagccgc ccgacagggg
    64921 ataccactgg ctatctggca aacccgaagg aggtgtgaga tgggaagtgg atggcatgaa
    64981 tggccgctga tgatcttcac ggtcttcggg caatgtgtgg cgggtggttt tatcgttctg
    65041 gctttggcgc tgctcaaagg cgacctgcga gcagaagccc agcagcgtgt tatcgcctgc
    65101 atgtttggct tatgggtact gatgggcatt ggctttatcg cctctatgct ccatcttggt
    65161 tcaccgatgc gcgcctttaa ctcgctcaac cgggtagggg cttcagcact cagtaacgaa
    65221 atcgccagcg gttcgatctt ttttgctgta ggcggcatcg gctggttgct ggcaatgctg
    65281 aaaaagctgt caccggcatt gcgtacgcta tggctggtgg tgacgatggt tcttggcgtc
    65341 gtctttgtct ggatgatggt gcgtgtgtat aacagcattg ataccgttcc gacctggtac
    65401 agcatctgga cgccgatggg cttcttcctg acgatgttca tgggcggccc gttactgggt
    65461 tacctgttgc tgagtctggc tggcgtcgat ggctgggcga tgcgtctgct gccagccatt
    65521 tctgtactgg cactggtggt gagtggcgtg atgtcagtga tgcagggcac agagctggca
    65581 actattcata gttctgtgca gcaggccgca gcgctggtgc cggactatgg tgcgctgatg
    65641 tcctggcgga tcgtgctttt ggccgttgcc ctgtgcttgt ggattgcacc acagctaaaa
    65701 ggttatcagc ctgcggttcc gctactatcc gtctcattca ttctgcttct ggcaggggaa
    65761 ttaatcggtc gcggcgtatt ctatggtttg catatgaccg tggggatggc cgtcgcaagc
    65821 taatcagtaa caaccggggt ttcggccccg gttctctttt atttctgctt cgttaacgtg
    65881 tcataactgg tcatcaaatt acgataatca ggaatatggt tagagaacaa cgtcgccagt
    65941 ccttcaatat cattacgcca gtcacggtgc agctcgcaag ccacgccgaa ccatgtcatc
    66001 aattgcgcac cagcttgcga catgcgatcc cacgccgaat gccgggtaat ttcattaaag
    66061 gtccctgatg catcagtcac gacaaagaca tcaaaccctt cttcaatggc tgaaagcgcc
    66121 gggaatgcca cgcaaacttc ggttaccaca ccggcaataa ttaactgttt tttacctgtc
    66181 gctttgactg cttttacaaa atcttcgtta tcccaggcgt taatattgcc cgggcgagca
    66241 atgtaaggtg catcggggaa ttgtgctttc agttctagaa ctaatgggcc gttaggaccg
    66301 gtttcaaaac tggtggtaag aatagttggt aagttgaaat acttagccag gtcacccagc
    66361 gccagcacgt tatttttaaa cttatcgggt tcgatatccc gtacaaggga aagtaaacca
    66421 gcctggtgat caacaagcaa aacagcagca tcatttttat caagacgaac atacggtttg
    66481 gtcatcctct tctcctttcg agagtcagtg gtacgtctga aatcggtcag acgcgatgca
    66541 ttgctctgaa agcatagacg ggaaatatga gtttgctgtg accatgaaat ttttcgactg
    66601 aacgaagcgt atgaaatttg tgttagatca ataaaaacaa tcagatgaga ttaatcatct
    66661 cttcagcatt agcgttatga atgagtaata taaatcgata ataccgccgc tgcgatatgc
    66721 gcgtattttc acccattgac aatgtttttg gcggtggcat gatgcgcatg aaatttgaac
    66781 ttcctcacgg ttttaattca tgtccacgta tacccggcct gtcatgcttt tgctgtctgg
    66841 cctgcttttg ttgactctgg cgatcgcggt gttaaataca ctcgtgccgc tttggctcgc
    66901 ccaggaacac atgtccacat ggcaggtagg cgttgtcagc tcatcctatt ttaccggcaa
    66961 ccttgtcggt acattgctga cagggtatgt cattaaacgc attggcttta accgcagcta
    67021 ttatctgtcc tccttcattt ttgctgctgg ctgtgccggc cttggcctga tgattggttt
    67081 ctggagctgg ttggcgtggc gttttgtcgc gggcgtcggc tgtgccatga tttgggtggt
    67141 tgttgagagc gcgctgatgt gcagtgggac gtcacgtaac cgtgggcgtt tgcttgctgc
    67201 gtatatgatg gtttattacg tgggaacgtt tttaggccag ttactggtca gcaaagtctc
    67261 aaccgagctg atgagcgtat tgccgtgggt tacaggtttg acattggcag ggatcttacc
    67321 gctgttgttt acgcgtgtcc tgaatcagca ggctgaaaac catgattcga cgtcaattac
    67381 ggcaatgcta aaactccgtc aggcgcggct tggcgtgaat ggctgcatta tctcaggaag
    67441 cgttctggga tctctatatg gcctgatgcc tctgtacctc aatcacaaag gggtgagcaa
    67501 tgccagtatt ggtttctgga tggcggtact ggtcagtgcg ggtatcctcg gacaatggcc
    67561 gattggacgt ctggcggata agtttggtcg actgctggtt ttgcgtgttc aggtctttgt
    67621 cgtcattctc ggcagtattg cgatgcttag ccaggcggcg atggccccag cgttattcat
    67681 cctcggtgcc gctggcttta cgctatatcc ggtggcgatg gcctgggctt gtgagaaagt
    67741 tgaacatcat caactggtgg cgatgaacca agccttactg ttgagctata ccgtgggaag
    67801 tctgcttggc ccgtcattta ccgctatgct aatgcagaat ttctccgata atttattgtt
    67861 tatcatgatc gccagcgtat cgtttatcta tttgctgatg ctgcttcgca acgccggtca
    67921 tacgccgaaa cccgttgctc acgtgtaaat gaattcaagc aaagtgtgaa cttactgttt
    67981 cacactctgc ttttttgttt cttctatctg acttgctttt ttccaaattt tattcgttta
    68041 aaaataaaat gtgcagcagg ttataatttt gcatttcgct atttccgcac ttcttatttg
    68101 ccgcgcataa tccctcgttt tacagatgcc cctttaattt tggcgaagga tttgtctatg
    68161 gctgggaatg ttcaggaagt aagcgcccca tc
//
LOCUS       DADMEQ010000007        67090 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.007, whole genome shotgun sequence.
ACCESSION   DADMEQ010000007 DADMEQ010000000
VERSION     DADMEQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.007"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(64..1068)
                     /gene="cra"
                     /locus_tag="JNZ05_000471"
     CDS             complement(64..1068)
                     /gene="cra"
                     /locus_tag="JNZ05_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HAY8563824.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1150..1236)
                     /locus_tag="JNZ05_000472"
     CDS             complement(1150..1236)
                     /locus_tag="JNZ05_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HAY8563825.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="JNZ05_000473"
     CDS             complement(1248..1739)
                     /gene="ilvN"
                     /locus_tag="JNZ05_000473"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAY8563826.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1742..3459)
                     /gene="ilvI"
                     /locus_tag="JNZ05_000474"
                     /pseudo
     CDS             complement(1742..3459)
                     /gene="ilvI"
                     /locus_tag="JNZ05_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025294.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
     gene            complement(3777..4721)
                     /gene="leuO"
                     /locus_tag="JNZ05_000475"
     CDS             complement(3777..4721)
                     /gene="leuO"
                     /locus_tag="JNZ05_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HAY8563827.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5382..5468
                     /gene="leuL"
                     /locus_tag="JNZ05_000476"
     CDS             5382..5468
                     /gene="leuL"
                     /locus_tag="JNZ05_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HAY8563828.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5561..7132
                     /gene="leuA"
                     /locus_tag="JNZ05_000477"
     CDS             5561..7132
                     /gene="leuA"
                     /locus_tag="JNZ05_000477"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HAY8563829.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPGHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7132..8223
                     /gene="leuB"
                     /locus_tag="JNZ05_000478"
     CDS             7132..8223
                     /gene="leuB"
                     /locus_tag="JNZ05_000478"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HAY8563830.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNKIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8226..9626
                     /gene="leuC"
                     /locus_tag="JNZ05_000479"
     CDS             8226..9626
                     /gene="leuC"
                     /locus_tag="JNZ05_000479"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HAY8563831.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKSGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9637..10242
                     /gene="leuD"
                     /locus_tag="JNZ05_000480"
     CDS             9637..10242
                     /gene="leuD"
                     /locus_tag="JNZ05_000480"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HAY8563832.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10291..11469)
                     /gene="setA"
                     /locus_tag="JNZ05_000481"
     CDS             complement(10291..11469)
                     /gene="setA"
                     /locus_tag="JNZ05_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HAY8563833.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11571..11701)
                     /gene="sgrT"
                     /locus_tag="JNZ05_000482"
                     /pseudo
     CDS             complement(11571..11701)
                     /gene="sgrT"
                     /locus_tag="JNZ05_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
     gene            11790..13445
                     /locus_tag="JNZ05_000483"
     CDS             11790..13445
                     /locus_tag="JNZ05_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SgrR"
                     /protein_id="HAY8563834.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGDLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13479..13578
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13609..14592
                     /gene="thiB"
                     /locus_tag="JNZ05_000484"
     CDS             13609..14592
                     /gene="thiB"
                     /locus_tag="JNZ05_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HAY8563835.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14568..16178
                     /gene="thiP"
                     /locus_tag="JNZ05_000485"
     CDS             14568..16178
                     /gene="thiP"
                     /locus_tag="JNZ05_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HAY8563836.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16162..16860
                     /gene="thiQ"
                     /locus_tag="JNZ05_000486"
     CDS             16162..16860
                     /gene="thiQ"
                     /locus_tag="JNZ05_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HAY8563837.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIVWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16974..17738)
                     /locus_tag="JNZ05_000487"
     CDS             complement(16974..17738)
                     /locus_tag="JNZ05_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8563838.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17824..18702)
                     /gene="araC"
                     /locus_tag="JNZ05_000488"
     CDS             complement(17824..18702)
                     /gene="araC"
                     /locus_tag="JNZ05_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HAY8563839.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19041..20740
                     /gene="araB"
                     /locus_tag="JNZ05_000489"
                     /pseudo
     CDS             19041..20740
                     /gene="araB"
                     /locus_tag="JNZ05_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
     gene            20751..22253
                     /gene="araA"
                     /locus_tag="JNZ05_000490"
                     /pseudo
     CDS             20751..22253
                     /gene="araA"
                     /locus_tag="JNZ05_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            22453..23148
                     /gene="araD"
                     /locus_tag="JNZ05_000491"
     CDS             22453..23148
                     /gene="araD"
                     /locus_tag="JNZ05_000491"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY8563840.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSDVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23223..25574
                     /gene="polB"
                     /locus_tag="JNZ05_000492"
     CDS             23223..25574
                     /gene="polB"
                     /locus_tag="JNZ05_000492"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HAY8563841.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWVRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25739..28645
                     /gene="rapA"
                     /locus_tag="JNZ05_000493"
     CDS             25739..28645
                     /gene="rapA"
                     /locus_tag="JNZ05_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HAY8563842.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNTVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28657..29316
                     /gene="rluA"
                     /locus_tag="JNZ05_000494"
     CDS             28657..29316
                     /gene="rluA"
                     /locus_tag="JNZ05_000494"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HAY8563843.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARAVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29433..30248)
                     /gene="djlA"
                     /locus_tag="JNZ05_000495"
     CDS             complement(29433..30248)
                     /gene="djlA"
                     /locus_tag="JNZ05_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HAY8563844.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30503..32857
                     /gene="lptD"
                     /locus_tag="JNZ05_000496"
     CDS             30503..32857
                     /gene="lptD"
                     /locus_tag="JNZ05_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HAY8563845.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32910..34196
                     /gene="surA"
                     /locus_tag="JNZ05_000497"
     CDS             32910..34196
                     /gene="surA"
                     /locus_tag="JNZ05_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HAY8563846.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKLSEEAASWMQEQRASAYVKILSN"
     gene            34196..35185
                     /gene="pdxA"
                     /locus_tag="JNZ05_000498"
     CDS             34196..35185
                     /gene="pdxA"
                     /locus_tag="JNZ05_000498"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HAY8563847.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGITEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35182..36003
                     /gene="rsmA"
                     /locus_tag="JNZ05_000499"
     CDS             35182..36003
                     /gene="rsmA"
                     /locus_tag="JNZ05_000499"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAY8563848.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36006..36383
                     /gene="apaG"
                     /locus_tag="JNZ05_000500"
     CDS             36006..36383
                     /gene="apaG"
                     /locus_tag="JNZ05_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HAY8563849.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36390..37232
                     /gene="apaH"
                     /locus_tag="JNZ05_000501"
     CDS             36390..37232
                     /gene="apaH"
                     /locus_tag="JNZ05_000501"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HAY8563850.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGMPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37310..37789)
                     /gene="folA"
                     /locus_tag="JNZ05_000502"
     CDS             complement(37310..37789)
                     /gene="folA"
                     /locus_tag="JNZ05_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HAY8563851.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37980..39842)
                     /gene="kefC"
                     /locus_tag="JNZ05_000503"
     CDS             complement(37980..39842)
                     /gene="kefC"
                     /locus_tag="JNZ05_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HAY8563852.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39835..40365)
                     /gene="kefF"
                     /locus_tag="JNZ05_000504"
     CDS             complement(39835..40365)
                     /gene="kefF"
                     /locus_tag="JNZ05_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HAY8563853.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40413..41807)
                     /locus_tag="JNZ05_000505"
     CDS             complement(40413..41807)
                     /locus_tag="JNZ05_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8563854.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41866..42153)
                     /gene="fixX"
                     /locus_tag="JNZ05_000506"
     CDS             complement(41866..42153)
                     /gene="fixX"
                     /locus_tag="JNZ05_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HAY8563855.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42150..43436)
                     /locus_tag="JNZ05_000507"
     CDS             complement(42150..43436)
                     /locus_tag="JNZ05_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY8563856.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADFAPVERLITHEKLAFMTEKSAMTMDYCNGDETSLSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIESPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESSPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43487..44428)
                     /locus_tag="JNZ05_000508"
     CDS             complement(43487..44428)
                     /locus_tag="JNZ05_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAY8563857.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQTRQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVRISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(44443..45213)
                     /locus_tag="JNZ05_000509"
     CDS             complement(44443..45213)
                     /locus_tag="JNZ05_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HAY8563858.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45684..47198
                     /locus_tag="JNZ05_000510"
     CDS             45684..47198
                     /locus_tag="JNZ05_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HAY8563859.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47229..48371
                     /gene="caiA"
                     /locus_tag="JNZ05_000511"
     CDS             47229..48371
                     /gene="caiA"
                     /locus_tag="JNZ05_000511"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HAY8563860.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48500..49717
                     /gene="caiB"
                     /locus_tag="JNZ05_000512"
     CDS             48500..49717
                     /gene="caiB"
                     /locus_tag="JNZ05_000512"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HAY8563861.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49791..51344
                     /gene="caiC"
                     /locus_tag="JNZ05_000513"
     CDS             49791..51344
                     /gene="caiC"
                     /locus_tag="JNZ05_000513"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HAY8563862.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51453..52238
                     /gene="caiD"
                     /locus_tag="JNZ05_000514"
     CDS             51453..52238
                     /gene="caiD"
                     /locus_tag="JNZ05_000514"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HAY8563863.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52244..52834
                     /gene="caiE"
                     /locus_tag="JNZ05_000515"
     CDS             52244..52834
                     /gene="caiE"
                     /locus_tag="JNZ05_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HAY8563864.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52920..53315)
                     /gene="caiF"
                     /locus_tag="JNZ05_000516"
     CDS             complement(52920..53315)
                     /gene="caiF"
                     /locus_tag="JNZ05_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HAY8563865.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53576..56797)
                     /gene="carB"
                     /locus_tag="JNZ05_000517"
     CDS             complement(53576..56797)
                     /gene="carB"
                     /locus_tag="JNZ05_000517"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAY8563866.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56815..57963)
                     /gene="carA"
                     /locus_tag="JNZ05_000518"
     CDS             complement(56815..57963)
                     /gene="carA"
                     /locus_tag="JNZ05_000518"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAY8563867.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58419..59240)
                     /gene="dapB"
                     /locus_tag="JNZ05_000519"
     CDS             complement(58419..59240)
                     /gene="dapB"
                     /locus_tag="JNZ05_000519"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAY8563868.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59407..60321)
                     /gene="rihC"
                     /locus_tag="JNZ05_000520"
     CDS             complement(59407..60321)
                     /gene="rihC"
                     /locus_tag="JNZ05_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HAY8563869.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60387..61337)
                     /gene="ispH"
                     /locus_tag="JNZ05_000521"
                     /gene_synonym="lytB"
     CDS             complement(60387..61337)
                     /gene="ispH"
                     /locus_tag="JNZ05_000521"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HAY8563870.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61339..61788)
                     /gene="fkpB"
                     /locus_tag="JNZ05_000522"
     CDS             complement(61339..61788)
                     /gene="fkpB"
                     /locus_tag="JNZ05_000522"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY8563871.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61875..62369)
                     /gene="lspA"
                     /locus_tag="JNZ05_000523"
     CDS             complement(61875..62369)
                     /gene="lspA"
                     /locus_tag="JNZ05_000523"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HAY8563872.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62369..65185)
                     /gene="ileS"
                     /locus_tag="JNZ05_000524"
     CDS             complement(62369..65185)
                     /gene="ileS"
                     /locus_tag="JNZ05_000524"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAY8563873.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAAILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65228..66169)
                     /gene="ribF"
                     /locus_tag="JNZ05_000525"
     CDS             complement(65228..66169)
                     /gene="ribF"
                     /locus_tag="JNZ05_000525"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HAY8563874.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66177..66395)
                     /locus_tag="JNZ05_000526"
     CDS             complement(66177..66395)
                     /locus_tag="JNZ05_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HAY8563875.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66498..66761
                     /gene="rpsT"
                     /locus_tag="JNZ05_000527"
     CDS             66498..66761
                     /gene="rpsT"
                     /locus_tag="JNZ05_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAY8563876.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16281 a        18251 c        17269 g        15289 t
ORIGIN      
        1 tgtccagtcc cgtactctac gcgccagagg gaaattcacc tggcgcgtat ttttgttcgc
       61 ggcttagcta cggctgagca cgccgcggcg atagagatta cgtttaatgc gcgttaaacc
      121 aggttttggc ttacgcggtt cgtccaggct tgccaggaca atctccagca cacgctctgc
      181 gacatcgcgg tgacgttgag ccactgccag caccggacac tgtaagaagt cgagcagttc
      241 gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt ttgccgtcgc gacgcaacgt
      301 gacatccatc actccttgca acaacgcaaa cgacgttgtg aacagcgcct gcggcatcgg
      361 atgcgtttcc agccattttt cgaataactg ggcagccgcc tcccgctcat agctgttggc
      421 atacaggaaa tgcacttcgc gcggatcatc tttccaagca gtacggaaac cttgttcacg
      481 caggaagctg acagaaagct ccggcagcgc gccaagataa agcaccgtct cggcgggaaa
      541 cttacgtaac tcttccgcca gcatttcggc atcatcctga tcggcaccaa ccacgctggt
      601 gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc gggaacgggt cgttagccca
      661 gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa acaataatgg catcaacctg
      721 acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct ggctgatctt ctgagcaggc
      781 aatcagcagt tgataacccc gttgccgcgc ctggcgttca agatagttag cgatgcgggt
      841 atagctggtg ttctccagat cggggatcac aagaccaata gaacgtgtgc gtccagcacg
      901 aagcccagct gccacggcgt tcgggtggta attgtgctca cgcaccacag ccatgacttt
      961 ttcaacggtt ttgtcgctca cacggtattg cttcgctttg ccgttaataa catagcttgc
     1021 agtggtccgc gacactcccg ccagccgagc gatttcatcc agtttcacaa ttgccccttg
     1081 cgtaaaatgt aaaaaccata gccattgtac tggtatgggt taaattcatt aacatctaag
     1141 cgcagaaaat taactgcggc aactgctttt attccaggtt cccactgatt tcgcaaaaaa
     1201 agcccaacgt gacatgttgg gctgcaaatt gcgcactgag atcattctca acgcattatt
     1261 ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa cttccacaat tttcgccaca
     1321 tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa ttgaacggta
     1381 taaagcgagg gggtgacatc gataatttgc ccacggaata tttccgtatt acgtttcact
     1441 tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca tgatttcccg ctcaacatgc
     1501 gcgccctgcc ccaactcact cacgcgcaag acatcgacca gcttgtgtaa ttgcttttcg
     1561 atctgctcaa gtactttttc atcgcccacg gtctggatgg tcatacgcga taatgtcgga
     1621 tcgtcggttg gcgcaacggt caggctttca atgttgtagc ctcgctggga aaaaaggcca
     1681 atcacgcggg ataacgcgcc tgattcattt tcgagtaaga ctgataatat ccggcgcata
     1741 atcaggttct ctccgttttg cttaaccaca tttcatccat tccgcccccg cgaatctgca
     1801 tcgggtagac gtgctcactg ccatcgacgg taacatcaac aaacaccagg cgattattgc
     1861 gcacctgttc cagcgcctcg ctaagtttgc tttccagctc atgcggatga gagatctgga
     1921 tcccgacgtg accataggct tccgccagac ggacgaaatc gggtagtgat tgcatataag
     1981 attgtgaatg acggccggaa tagatcatgt cctgccactg tttcaccatc cccagatagc
     2041 ggttattgag attcaccacc agtacgggca actcgtattg caacgcggta gacagttcct
     2101 ggatgttcat ctgaatactg ccgtcgccag tgacgcaaac cacggtttct tctggcaacg
     2161 ccattttgac gcccagtgcc gcaggtaaac caaaacccat cgtgccgaga ccgccggaat
     2221 tgatccagcg acgcggtttg tcgaatggat aataaagcgc agcaaacatc tggtgctgcc
     2281 cgacatcgga cgtcacgtaa gcgtccccat tagtcaaccg ccaaagagtc tcgatcaccg
     2341 cctgcggttt aatcttttca ctgtgagtgt catatttcag gcactgaaga gcgcgccact
     2401 gttcaatctg ctgccaccag tcgcggatct catccagcgg ttgatgggcg gattcttgcg
     2461 acaggagttc aagcatttgt tcgaggacct ggcgagcatc ccccacaatc gggatatccg
     2521 cagtcacggt tttagaaatg gaagtaggat caatatcgat atgcagaaca gtggcatttg
     2581 ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa tcgtaccccg acggcgaaaa
     2641 tcacatccgc gttatgcatc gtcatattgg cttcgtaggt accgtgcatt cccagcatgc
     2701 ccagtgcctg acgatgcgtt gccggaaacg cccccagccc catcaatgag gaaacaacgg
     2761 gcagattcaa cgcctccacc gtttctttca actgctgatg gcagcccgcc atgattgccc
     2821 caccgcctac gtagacaacc ggtttttttg ccgctaccag cgtttgcaga gcacgcttaa
     2881 tttgcccttt atgtccggta gtagtgggat tgtaagaacg catactgacc gactcagggc
     2941 agacataggg taatttgttc gccggattaa gaatatcttt cggtaaatca acgaccactg
     3001 gtcctgggcg accacttgcc gccagccaga aagccttttt cagcacctgc ggaatgtctt
     3061 ccgtttgctt aaccagaaaa ctgtgtttaa ccaccggtcg cgaaatcccc accatgtcgc
     3121 actcctgaaa agcatcgtaa cctatcaacg aggtcgctac ctgcccggaa aggacaacta
     3181 atggaatgga atccatataa gcggtggcga tgccggtaat cgcattggtc gcccctggac
     3241 ccgacgttac cagcacgacg ccgacttccc cggtcgcacg cgccaggcca tcggccatat
     3301 ggcctgctca tgacgaacta atacatgatc aataccaccc acggtatgca atgcatcgta
     3361 aatatcaagg actgcgcctc cgggataacc gaatacttgt ttaacgccct gatcgataag
     3421 cgatcggacg accatctcgg ctccagacaa catctccatg gcctgcctca ctgtttgacg
     3481 gaaaaaatgt gtaaaacaca ttaaccgtcc gatcttgctt aagcaattgg cagccagaga
     3541 cataaatgca gggtaataag aataaacagg aggaaaggtg aaaagaaacg ataaaatcct
     3601 ccattacgaa ggattaaatt gataataaat cggctgaatc ccacaactta cagcaatttc
     3661 tctatttttt taattaatcc taaaatagag agtccacaca ttcagaataa aattctacat
     3721 tcaataaaaa atgcagaata aaccagacat tcatgtctgg cctattctgc attcagttag
     3781 cgtttgcaaa ttgagactaa ttgttcttcc atccactgat ggcctttatc gcgcccggca
     3841 gcttcatgcc aagagagata acaggttctg ctgttttgtt ttaacggcag cggtaatacc
     3901 tgtaattcta aggattcagc gaactcttca gccagccaac gcggcgcaat agcgaccaaa
     3961 tgcgtttgcg acaccacgct aagtacgctc atcattgcca tgccctgata cgcgatactg
     4021 gcttgcttat ctaccgtgtc ataccaaggt tgactaaatg acgcgaaacg atcgagcgaa
     4081 accgccgcat gttgttcgtt ataaacatca tgtttcagta acgggccttt aattgttgga
     4141 tgatttttgc tggctaccag caccatttca tctttaaata atggcacgct ggtaaattca
     4201 ggacgatgga agtcttcata actaatcaca aactccgttt cctgataacg cagctgatgt
     4261 tcagtgttct gatttaatga agacttgaac ataacatgta tatttggcgc aatctgctca
     4321 atgtgattat aaatctgcga ggtcagaatg ctgtctaacg ggctgcaaac acaaagatga
     4381 aatacacgtt cactgctcgc gggttcaaaa cctgaaccag gcaattcatt ttgtactagt
     4441 tgcaatgcct ggcgaactga accaaaaagt tgaaatgcgc gagcagtcgg ttgaatacca
     4501 cggccataac gaacaaaaag ctcgtcatta aacatcacct tcaggcgtgc aacagcgtta
     4561 ctgaccgcag gttgcgacat acccagaaca tgagcggcac gggtaatgtt ttgctcctgc
     4621 atcacggcat cgaaaacggt taataagttg agatcgacca tgcgtagctg tggcttgctt
     4681 aactccgccg tctctgaatg atctgtttgt acctctggca tacttaactc cactgtcaca
     4741 cttaactccc tttcccttat tggaatgcag agaaataatc ctgaagaata tgatttatca
     4801 tgcatataaa ataagaaaaa gcaaaatgag taaaattcgg aaaacataaa gatacgggtt
     4861 ctgataaaaa cagaatccat tgccgtggaa tgagtcattt accacagcat aataatccat
     4921 aatgtttttg ttgcgaaaac aatctaatca taactaccgc gaatactcaa tcatctacaa
     4981 aatggattaa atgtgcttat ttaataatta ttttatatat ttaatgcatt aattcttaac
     5041 attaattgat caataatatt caccaaatca atatcaaaaa aaaatcgcaa aacatataat
     5101 tcaatacaaa tcatcaggat aggttttgca acgcgtgcat tttgtcccct ttttcctcgt
     5161 tgattagatg caaaaattta tgctgaaata tgtcaaccga tgaaaagcgt cggtagttaa
     5221 gcagaaatta atatcgctta ctttaaccac cgtagcacaa ttagctaatt ttacggatgc
     5281 agaactcacg ctggcgggac gtttttattg cgtcagggtt gacatccgtt tttgtatcca
     5341 gtaactctaa aagcatatcg cattcatctg gagctgattt aatgactcac atcgttcgct
     5401 ttatcggtct actactacta aacgcatctt ctttgcgcgg tagacgagtg agcggcatcc
     5461 agcattaagc cagcacgcag tcaaacaaaa aacccgcgcc attgcgcggg tttttttatg
     5521 cccgaagcga ggcgctctaa aagagacaag gacccaaacc atgagccagc aagtcattat
     5581 tttcgatacc acattgcgcg acggtgaaca ggcgttacag gcaagcttga gtgtgaaaga
     5641 aaaactgcaa attgcgctgg cccttgagcg tatgggtgtt gacgtgatgg aagtcggttt
     5701 ccccgtctct tcgccgggcg attttgaatc ggtgcaaacc atcgcccgcc aggttaaaaa
     5761 cagccgcgta tgtgcgttag ctcgctgcgt ggaaaaagat atcgacgtgg cggccgaatc
     5821 cctgaaagtc gccgaagcct tccgtattca tacctttatt gctacctccc caatgcacat
     5881 cgccaccaag ctgcgcagca ccctggacga ggtgatcgaa cgcgctatct atatggtgaa
     5941 acgcgcccgt aattacaccg atgatgttga attttcttgc gaagatgccg ggcgtacacc
     6001 cattgccgat ctggcgcgag tggtcgaagc ggcgattaat gccggtgcca ccaccatcaa
     6061 cattccggac accgtgggct acaccatgcc gtttgagttc gccggaatca tcagcggcct
     6121 gtatgaacgc gtacctaaca tcgacaaagc cattatttcc gtacataccc acgacgattt
     6181 aggcctggcg gtcggcaact cactggcggc ggtacatgcc ggtgcacgcc aggtggaagg
     6241 tgcaatgaac gggatcggcg agcgtgccgg taactgttcg ctggaagaag tcatcatggc
     6301 gattaaagtt cgtaaggata ttctcaacgt ccacaccgcc attaatcacc aggagatatg
     6361 gcgcaccagc cagttagtta gccagatttg taatatgccg atcccggcaa acaaagccat
     6421 tgttggcagc ggcgcattcg ctcactcctc cggtatccac caggatggcg tgctgaaaaa
     6481 ccgcgaaaac tacgaaatca tgacaccaga atctattggt ctgaaccaaa tccagctgaa
     6541 tctgacctct cgttcggggc gtgcggcggt gaaacatcgc atggatgaga tggggtataa
     6601 agaaagtgaa tataatttag acaatttgta cgatgctttc ctgaagctgg cggacaaaaa
     6661 aggtcaggtg tttgattacg atctggaggc gctggccttc atcggtaagc agcaagaaga
     6721 gccggggcat ttccgtctgg attacttcag cgtgcagtct ggctctaacg atatcgccac
     6781 cgccgccgtc aaactggcct gtggcgaaga agtcaaagca gaagccgcca acggtaacgg
     6841 tccggtcgat gccgtctatc aggcaattaa ccgcatcact gactataacg tcgaactggt
     6901 gaaatacagc ctgaccgcca aaggtcacgg taaagatgcg ctgggtcagg tggatattgt
     6961 cgccaactac aacggtcgcc gcttccacgg cgtcggcctg gccaccgata ttgtcgagtc
     7021 ctccgccaaa gccatggtgc acgtactgaa caatatctgg cgtgccgcag aagtcgaaaa
     7081 agagttgcaa cgcaaagctc aacacaacga aaacaacaag gaaaccgtgt gatgtcgaag
     7141 aattaccata ttgccgtatt gccgggagac ggtattggtc cggaagtgat gacccaggcg
     7201 ctgaaagtgc tgaatgccgt gcgcaaccgc tttgcgatgc gcatcaccac cagccattac
     7261 gatgtaggcg gcgcagccat tgataaccac gggcaaccac tgccgcctgc gacggttgaa
     7321 ggttgtgagc aagccgatgc cgtgctgttt ggctcggtag gcggcccgaa gtgggaacat
     7381 ttaccaccag accagcaacc agaacgcggc gcgctgctgc cgctgcgtaa gcacttcaaa
     7441 ttattcagca acctgcgccc ggcaaaactg tatcaggggc tggaagcatt ctgtccgctg
     7501 cgtgcagaca ttgccgctaa cggcttcgac atcctgtgcg tgcgcgaact gaccggcggc
     7561 atctatttcg gtcagccaaa aggccgcgaa ggtagcggac aatatgaaaa agcgtttgat
     7621 accgaggtgt atcaccgttt tgagatcgag cgtattgccc gcatcgcgtt tgaatctgct
     7681 cgtaaacgtc gccacaaagt cacctcaatc gacaaagcta acgtgctgca atcctctatt
     7741 ttatggcgtg aaatcgtcaa caagatcgcc acggaatacc cggatgtcga actggcgcat
     7801 atgtacatcg acaacgccac catgcagctg attaaagatc catcacagtt tgacgttctg
     7861 ctgtgctcca acctgtttgg cgacattctg tctgacgagt gcgcaatgat cactggctcg
     7921 atgggcatgt tgccttccgc cagcctgaac gagcaaggtt ttggactgta tgaaccggcg
     7981 ggcggctcgg caccagatat cgcaggcaaa aacatcgcca acccgattgc acaaatcctg
     8041 tcgctggcac tgctgctgcg ttacagcctg gatgccgatg atgcggcttc cgccattgaa
     8101 cgcgccatta accgtgcatt agaagaaggc attcgcaccg gggatttagc ccgtggcgct
     8161 gccgccgtta gtaccgatga aatgggcgat atcattgccc gctatgtggc agaaggggtg
     8221 taatcatggc taaaacgtta tacgaaaaat tattcgacgc tcacgtagtg tacgaagccg
     8281 aaaacgaaac cccgctgtta tatatcgacc gccacctggt gcatgaagtg acctcaccgc
     8341 aggcgttcga tggtctgcgc gcccacggtc gcccggtacg tcagccgggc aaaaccttcg
     8401 ccactatgga tcacaacgtc tctacccaga ccaaagacat taatgcctgt ggtgaaatgg
     8461 cgcgcatcca gatgcaggag ctgattaaaa actgcaaaga atttggcgtc gagctgtatg
     8521 acctgaatca cccgtatcag gggatcgtcc acgtaatggg gccggaacag ggcgtcacct
     8581 tgccggggat gaccattgtc tgcggcgact cgcataccgc cacccacggc gcgtttggcg
     8641 cactggcctt tggtatcggc acttccgaag ttgaacacgt actggcaaca caaaccctga
     8701 aacagggccg tgcgaagacc atgaaaattg aagtccaggg caaagccgcg ccgggcatta
     8761 ccgcaaaaga tatcgtgctg gcaattatcg gtaaaactgg cagcgcaggc ggcaccgggc
     8821 atgtggtgga gttttgcggc gaagcaattc gtgatttaag catggaaggt cgtatgaccc
     8881 tgtgcaatat ggcgatcgaa atgggcgcaa aatccggtct ggttgctcct gacgaaacca
     8941 cctttaacta tgtcaaaggc cgtctgcatg cgccgaaagg caaagatttc gacgacgccg
     9001 ttgcctactg gaaaaccctg caaaccgacg aaggcgcaac tttcgatacc gttgtcactc
     9061 tgcaagcaga agaaatttca ccgcaggtca cctggggcac caatcccggc caggtgattt
     9121 ccgtgaacga caatattccc gatccggctt cgtttgccga tccggttgaa cgtgcgtcgg
     9181 cagaaaaagc gctggcctat atggggctga aaccgggtat tccgctgacc gaagtggcta
     9241 tcgacaaagt gtttatcggt tcctgtacca actcgcgcat tgaagattta cgcgcggcag
     9301 cggagatcgc caaagggcga aaagtcgcgc caggcgtgca ggcactggtg gttcccggct
     9361 ctggcccggt aaaagcccag gcggaagcgg aaggtctgga taaaatattt attgaagccg
     9421 gttttgaatg gcgcttgcct ggctgctcaa tgtgtctggc gatgaacaac gaccgtctga
     9481 atccgggcga acgttgtgca tccaccagca accgtaactt tgaaggccgc caggggcgcg
     9541 gcgggcgcac gcatctggtc agcccggcaa tggctgccgc tgctgctgtg accggacatt
     9601 tcgccgacat tcgcaacatt aaataaggag cacaccatgg cagagaaatt tatcaaacac
     9661 acaggcctgg tggttccgct ggatgccgcc aatgtcgata ccgatgcaat catcccgaaa
     9721 cagtttttgc agaaagtgac ccgtacgggt tttggcgcgc atctgtttaa cgactggcgt
     9781 tttctcgatg aaaaaggcca acagccaaac ccggacttcg tgctgaactt cccgcagtat
     9841 cagggcgctt ccattttgct ggcacgagaa aacttcggct gtggctcctc gcgtgagcac
     9901 gcgccctggg cattgaccga ctacggtttt aaagtggtga ttgcgccgag ttttgctgac
     9961 atcttctacg gcaatagctt taacaaccag ctgctgccgg tgaaattaag cgatgcagaa
    10021 gtggacgaac tgtttgcgct ggtgaaagcc aatccgggga tccatttcga cgtggatctg
    10081 gaagcgcaag aggtgaaagc gggagagaaa acctatcgct ttaccatcga tgccttccgc
    10141 cgccactgca tgatgaatgg tctggacagc atcggcctga ccctgcagca cgacgacgcg
    10201 attgcgtctt atgaagcaaa acaacctgcg tttatgaatt agcccccttg cccggtcaaa
    10261 tgaccgggct ttccgctatc gtccacgtca tcaaacgtct ttaacctttg cggttaaaaa
    10321 taatgcgaca acagaaataa tcgcaattac ccagtagaca gcaaagtgcc cccaactttg
    10381 tgcaattgct ccctgaataa caccagccag aattaccccg gtagagatag tgttggtaaa
    10441 taaggtggta gctgcccccg ctcttccagg cattaaatcc tgaaaccata gcatcccaat
    10501 acccgcaaca atgccgataa atacagcgtt aaaaagttgc agcgtcatca acgccatacg
    10561 gctatgaaag aaaatcaatc cagtgtaaaa cagcactcct gccaccactg caatgaccat
    10621 cattcgccgc ttaccataac gtttgacata gtagccagcc agaatcattg ctggtatttc
    10681 cagtccagct gccgtcccca tcaggaaacc cgccagtttg tctggcaatc ctaaatcgct
    10741 actgatccac aacggcatat caataatgta catagtgttg caggtccaca ttaacgtcga
    10801 ggcgacaaat aacatccgta cgttactatc ctgccagtca ccttgcattg ataaagcatt
    10861 ttccgacggc agttctaccc gcgccacaga cggaagcata aatgcaatca ataccagact
    10921 gagtgtgaat atcccggcgg caatcgaaaa catcactgta aagccgtaat tcaacgccag
    10981 cataaaggcc agcggtggac cgattaccca tgccagagaa agctgcgcac gcatcaccga
    11041 gctaaacatc accacttctc gcgccgagtt atccgcgtat tcccgcgcca gagcaaataa
    11101 ctgtggcatt gccgtattgg ccagagatgc cagaagcaca ccacaggtga taagcgtcag
    11161 ataatgacga ttaaatgcaa acagtagcgc attgccaata gccatcaaac agcaaaatat
    11221 aatcagtttt cgccgatcgc cctgactgtc agaacgtttt gccaaccaga ggcttacgcc
    11281 gatcccagca atagcattca ccgtataaaa gagtccgatc cagaaaggtt gcgcgccaac
    11341 ctcacgactc agaaataagc tcaatgtagg agcctgtagc gccccggcca cccccatcat
    11401 aaaagcgacc agcataaatg ccgcgtaaac accgttcata cggcgagcca tcgtcattat
    11461 ccagatcata cgttcccttt ttagcgcggc gagaataaaa aaaaccagca ggtataatct
    11521 gctggcgggt gattttacac caatactcag tcacacatga tgcaggcaag tcaactttca
    11581 gaattgcggt catcccactg catcagtcct tcaagcatgg ttaatcgttg tggacgctta
    11641 accaacgcaa ccagcacaac ttcgctgtcg cggtaaaata gtgctgataa aactgacgca
    11701 tggggcatct ccttgcttca tcgttaggaa ttattgactt aatataggga aaataaaatt
    11761 gctgtctttt gcgcaggagt tcccctttta tgccatctgc tcgtctgcaa caacagttca
    11821 tccgcctgtg gcaatgctgc gagggtaaat cgcaggacac aacgctcaac gaactggcag
    11881 cgttattgag ctgctcgcgt cgtcatatgc gtaccctgct caacaccatg caggatcgcg
    11941 gctggctgac gtgggaagcg gaagtcgggc gcggtaaacg ctcgcgtctg acattcctct
    12001 ataccgggct ggcgcttcag caacagcggg cggaagacct gctggagcag gatcgtatcg
    12061 accaactagt gcagttggtt ggcgacaaag cgactgtgcg gcaaatgctg gtttctcatc
    12121 tgggccgcag cttccgccag gggcggcaca tcctgcgcgt gctctactat cgtccgttgc
    12181 gtaatctgct acctggcagc gcattgcgcc gttccgaaac ccatattgcc cggcaaatct
    12241 tcagttcgct aacgcgcata aatgaggaaa atggggaact ggaagcagac atcgcccacc
    12301 actggcagca aatatcaccg cttcactggc gtttcttttt gcgtccagga gtccattttc
    12361 atcatggtcg tgaactggaa atggacgacg tgatcgcctc tttaaaacga atcaatacgc
    12421 tgccgctcta ttcgcatatt acggacattg tgtcgccgac gccctggacg ctggatatcc
    12481 atctcacgca gccggaccgc tggttgccgt tactgctggg gcaagttccg gcgatgatcc
    12541 tgccgtgcga atgggaaacc ctcagtaact ttgccagcca tcctattggc accggtccgt
    12601 atgcggtgat tcgcaacagc accaatcaac tgaaaattca ggcattcgat gacttcttcg
    12661 gttaccgggc attaatcgac gaagttaacg tctgggttct gccggaaatt gccgacgagc
    12721 cagccggtga tctgatgcta aaaggtccac agggcgagga aaaagagatt gaaagccgcc
    12781 tggaggaagg ttgctactat ttactgttcg acagccgcac ccatcgcggg gcgaatcagc
    12841 aagtcaggga ctgggtaagc tatgtgcttt ctccaactaa tctggtctat ttcgctgagg
    12901 aacagtacca gcaactgtgg ttcccggctt atggactgct cccccgttgg caccatgccc
    12961 gcaccataaa gagcgaaaaa ccggctggcc tggaaagcct caccctgacc ttttatcagg
    13021 atcacagtga gcatcgggtg attgccggga tcatgcagca gattctggca agtcaccagg
    13081 tcacgctgga aatcaaagag atcagctacg atcagtggca tgaaggagag atcgagagcg
    13141 atatctggct taatagcgcc aactttacgc tgccgctgga tttttcgctg ttcgcgcact
    13201 tgtgcgaggt gccgctgctc caacactgta ttccgatcga ctggcaagcc gacgccgccc
    13261 gctggcgcaa tggcgaaatg aacctggcga actggtgcca gcaactggtc gccagtaaag
    13321 caatggtgcc gcttatccac cactggctga tcattcaggg acaacgcagt atgcgcggcc
    13381 tgcgtatgaa taccctcggc tggttcgatt ttaaatcagc gtggtttgcg ccaccggatc
    13441 catgattgct ggtagataac aaaatcacta cactaacgcc gttctcaacg gggtgccacg
    13501 caaacgcgtg cgctgagaaa atacccgtcg aacctgatcc ggataacgcc ggcgaaggga
    13561 tttgaggctc cttctcaagt cctttgccac tcttttttga ggtgcaaagt gttaaaaaaa
    13621 tgtcttcccc tgctgttgct gtgcacagcg cccgttttcg ctaaacccgt tctgactgtt
    13681 tatacctacg attccttcgc cgccgactgg gggcctggtc caaagatcaa aaaagctttt
    13741 gaagccgact gtaactgcga actgaaactg gtggcgctgg aagatggcgt ttcgcttctc
    13801 aaccgtctac ggatggaagg caaaaatagt aaagccgatg tggtgctggg gctggataac
    13861 aatctgttag acgccgccag taaaaccgga ctatttgcca aaagcggtgt ggcagcggat
    13921 gccgttaacg ttcccggcgg ctggaataat gacactttcg tcccgtttga ttacggctat
    13981 ttcgccttcg tctatgacaa gaacaaactg aaaaacccgc cgcaaagcct gaaagaattg
    14041 gttgagagcg atcagaactg gcgggtgatt tatcaggatc cgcgtaccag cacgccgggg
    14101 ctgggtctgc tgttgtggat gcaaaaagtc tatggcgatg acgccccaca agcctggcaa
    14161 aaactggcga agaaaaccgt cacggtcacc aaaggctgga gcgaagccta cggcctgttt
    14221 ttaaaaggtg aaagcgatct ggtactgagt tacaccacct ctccggctta tcacattctc
    14281 gaagagaaaa aagataacta cgccgccgcg aactttagcg aaggtcacta tctgcaggtg
    14341 gaagtcgccg cccgcaccgc tgccagcaag cagccggagc tggcgcaaaa attcctccag
    14401 tttatggttt ctccggcttt tcagaatgcg atcccaaccg gtaactggat gtatccggtg
    14461 gcaaatgtca cgctgcctgc cgggtttgaa caattgacca aaccggccac cacgctggag
    14521 ttcacaccag ctgaagtggc ggcacaacgt caggcatgga ttagcgaatg gcaacgcgcc
    14581 gtcagccgtt aattcccggc tggttaattc caggtgtaag cgccgccacg ctggtggtag
    14641 cggttgcgct cgcggcgttt ctcgccctgt ggtggaacgc gccgcagggt aactgggtgg
    14701 cagtctggca ggacagctac ctgtggcatg tggtgcgctt ctccttctgg caggcgtttc
    14761 tctcggcgct actctctgtc gtacccgcga tattcctcgc ccgcgccctc tatcgcaggc
    14821 gctttccggg tcggctggcg ctgttgcgtc tgtgcgcaat gaccttgatc ctcccggtgc
    14881 tagttgctgt tttcggcatt cttagcgtct atggtcgtca gggctggctg gcatcgctct
    14941 gccaatcgct cggtctggag tggacctttt cgccctacgg cctgcaaggc attttgctgg
    15001 cgcacgtatt ttttaatctg ccgatggcga gccgcttatt actccaggca ctggaaaaca
    15061 ttcccggcga acaacgtcag cttgccgccc agcttgggat gcgcggctgg cattttttcc
    15121 gcttcgtcga atggccgtgg ctacggcgac aaatcccgcc ggttgccgcg cttatcttta
    15181 tgctctgttt cgccagcttc gccaccgtgc tatcgctggg cggcggtccg caggcaacca
    15241 ctatcgagct ggctatttat caggcgctga gttacgacta cgatcctgcc cgcgcggcga
    15301 tgctggcgct gctccagatg gtgtgttgcc ttgggctggt gctgttgagt cagcgactaa
    15361 gtaaggccat tgcgcccggc accacgctgc tgcaaggctg gcgcgatccg gacgatcgtc
    15421 tgcatagccg catttgcgac acggtgttaa tagtgctggc gctgctgctg ttgctgccac
    15481 cgttgctggc ggtgatcgtc gatggggtaa atcgccagtt gccggaagtg ctggcacaac
    15541 cggtgctgtg gcaggcgctg tggacctcgt tgcgtattgc gctggcggca ggtgtattgt
    15601 gcgtagtgct gaccatgatg ctgctatgga gcagtcgcga actgcgggcg cggcagaaaa
    15661 tgctggcggg tcaggcgctg gagatgagcg gcatgttgat cctcgccatg ccggggattg
    15721 tgctggctac cggcttcttt ttactgctca acaacactat cggcctgcca caatctgctg
    15781 acggcattgt gattttcacc aatgcgttaa tggcgatccc ttatgcgctg aaagtactgg
    15841 aaaacccgat gcgcgatatc accgcccgct acagtatgtt gtgtcagtcg ctgggcattg
    15901 aaggctggtc gcgcttaaaa gtggtcgagc tgcgcgccct gaaacgtcca ctggcgcagg
    15961 cgctggcttt tgcctgcgtg ctgtcgattg gtgattttgg cgtagtggcg ttgttcggta
    16021 acgatgattt ccgtaccctg ccgttttatc tctaccagca aattggctcc tatcgcagcc
    16081 aggacggcgc ggtcaccgcg ttaattctgc tactactctg ttttctgctg tttaccgtga
    16141 ttgaaaaact accggggcga aatgttaaaa ctgactgata tcacctggct ttatcaccat
    16201 ttgccgatgc gttttagctt aacggtagaa cgcggcgagc aggtggcaat cctcgggcca
    16261 agcggcgcgg gtaaaagtac cctgctgaat ttgattgccg gttttctgac gccagccagc
    16321 ggttcgctga ctatcgatgg cgttgatcac accacgacgc cgccgtcacg ccgtccggtg
    16381 tcgatgctgt ttcaggagaa caacctgttc agccacctga cggtcgcgca gaacatcggg
    16441 ctggggctaa atccggggtt gaaactgaac gcggcacagc aggagaaaat gcacgcgatt
    16501 gcccgtcaga tggggattga taatttaatg gcgcggttac cgggcgagct ttccggcggt
    16561 cagcgacagc gcgtggcgtt agcgcgttgt ctggtgcgcg aacagccgat tttattgctc
    16621 gatgaaccgt tctctgcgct cgatccggcg ttacgtcagg agatgctgac gctggtgagc
    16681 acgagctgcc agcagcaaaa aatgacgcta ttgatggtgt cgcacagtgt ggaagatgcg
    16741 gcgcggatcg ccacacgctc ggtggtggtc gccgacgggc gcatcgtctg gcagggtaag
    16801 actgaagaat tgttgagcgg taaggcgagt gcttcggcaa tattggggat tacgggttag
    16861 tatgcactgt cagatgcggc gtgaacacct tatccgacct acggacagtg cgttttgtag
    16921 gcctgataag acgcggtcag cgtcgcatga ggcaacccgc acaagacaat cccctaaacc
    16981 ccaaccactt tacgcaaaat atcgatatac accggcatca acgggtggcg aattaacacc
    17041 accagcgcca ccacgccgat ggcagaaatc aacggcgtca gccacaacaa acggccgcgc
    17101 gacaaataat gacttaaacg atcggttgct ttaccgctgc gccataaccg ccagcacagc
    17161 cagccaccaa cccacaaaaa taccgccgtc gccagcagca accatttaaa ctcgccgctc
    17221 tgcattccgg ctggaatatc gatcgccgcg cccgccagaa tcccaggcag gaagtaaaac
    17281 ggtggccaca gcaggcagcc gataatattc ggcgtaatga attttgccac cggcagatcc
    17341 agcattcccg ccaccattgg caccagcgga cgcgtcgggc caacaaagcg accgaccaga
    17401 atggtgaaca tgctgtgttg atgcaacgca tgttcagttt tatcgagtaa tgctttgttt
    17461 ttcttcagaa atgaccagcg atgcaacggc tttttaaaac gccagcccag ccagaaggag
    17521 atccagtcgc ccagcagaca accgacaatc cctgccagcc aagcgtgcca gaaacttaac
    17581 tcgccgctgc caatcagcgc tcccagcccc gccatcagca ccgtaccggg tagaatcaaa
    17641 ccgaccagcg ccagcgactc cagaaaggcc accaacacca ccgccatcaa tgaatacacg
    17701 gtagattggg taataaagtg ttccagcaat gcttgcataa tgtgcctgtc aaatggacga
    17761 agcagggatt ctgcaaaccc tatgctactc cgtcaagccg tcaattgtct gattcgttac
    17821 caattatgac aacttgacgg ctacatcatt cactttttct tcacaaccgg cacggaactc
    17881 gctcgggctg gccccggtgc attttttaaa tacccgcgag aaatagagtt gatcgtcaaa
    17941 accaacattg cgaccgacgg tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc
    18001 ctggctgata cgttggtcct cgcgccagct taagacgcta atccctaact gctggcggaa
    18061 aagatgtgac agacgcgacg gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa
    18121 attgctgtct gccaggtgat cgctgatgta ctgacaagcc tcgcgtacac gattatccat
    18181 cggtggatgg agcgactcgt taatcgcttc catgcgccgc agtaacaatt gctcaagcag
    18241 atttatcgcc agcagctccg aatagcgccc ttccccttgc ccggcgttaa tgatttgccc
    18301 aaacaggtcg ctgaaatacg gctggtgcgc ttcatccggg cgaaagaaac ccgtattggc
    18361 aaatattgac ggccagttaa gccattcatg ccagtaggcg cgcggacgaa agtaaaccca
    18421 ctggtgatac cattcgcgag cctccggatg acaaccgtag tgatgaatct ctcctggcgg
    18481 gaacagcaaa atatcacccg gtcggcagac aaattctcgt ccctgatttt tcaccacccc
    18541 ctgaccgcga atggtgagat tgagaatata acctttcatt cccagcggtc ggtcgataaa
    18601 aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc gccaccagat gggcattaaa
    18661 cgagtatccc ggcagcaggg gatcattttg cgcttcagcc atacttttca tactcccgcc
    18721 attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact gcgtctttta
    18781 ctggctcttc tcgctaaccc aaccggtaac cccgcttatt aaaagcattc tgtaacaaag
    18841 cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt
    18901 ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt tatccataag
    18961 attagcggat cctacctgac gctttttatc gcaactctct actgtttctc catacccgtt
    19021 tttttggatg gagtgaaacg atggcgattg caattggcct cgattttggc agtgattctg
    19081 tgcgagcttt ggcggtggac tgcgccagcg gtgaagagat cgccaccagc gtagagtggt
    19141 atccccgttg gcaaaaaggg caattttgtg atgccccgaa taaccagttc cgtcatcatc
    19201 cgcgtgacta cattgagtca atggaagcgg cactgaaaac cgtgcttgca gagcttagcg
    19261 tcgaacagcg cgcagctgtg gtcgggattg gcgttgacag taccggctcg acgcccgcac
    19321 cgattgatgc cgacggtaac gtgctggcgc tgcgcccgga gtttgccgaa aacccgaacg
    19381 cgatgttcgt attgtggaaa gaccacactg cggttgaaga agcggaagag attacccgtt
    19441 tgtgccacgc gccgggcaac gttgactact cccgctatat tggcggtatt tattccagcg
    19501 aaggttctgg gcaaaaatcc tgcatgtgac tcgccaggac agcgccgtgg cgcaatctgc
    19561 cgcatcgtgg attgagctgt gcgactgggt gccagctctg ctttccggta ccacccgccc
    19621 gcaggatatt cgtcgcggac gttgcagcgc cgggcataaa tctctgtggc acgaaagctg
    19681 gggcggcttg ccgccagcca gtttctttga tgagctggac ccgatcctca atcgccattt
    19741 gccttccccg ctgttcactg acacctggac tgccgatatt ccggtgggca ccttatgccc
    19801 ggaatgggcg cagcgtctcg gcctgcctga aagcgtggtg atttccggcg gcgcgtttga
    19861 ctgccatatg ggcgcagttg gcgcaggcgc acagcctaac gcactggtaa aagttatcgg
    19921 tacttccacc tgcgacattc tgattgccga caaacagagc gttggcgagc gggcagttaa
    19981 aggtatttgc ggtcaggttg atggcagcgt ggtgcctgga tttatcggtc tggaagcagg
    20041 ccaatcggcg tttggtgata tctacgcctg gtttggtcgc gtactcggct ggccgctgga
    20101 acagcttgcc gcccagcatg cggaactgaa agcgcaaatc aacgccagcc agaaacaact
    20161 gcttccggcg ctgaccgaag catgggccaa aaatccgtct ctggatcacc tgccggtggt
    20221 gctcgactgg tttaacggtc gtcgcacgcc aaacgctaac caacgcctga aaggggtgat
    20281 taccgatctt aacctcgcta ccgacactcc gctgctgttc ggcggtttga ttgctgccac
    20341 cgcctttggc gcacgcgcaa tcatggagtg ctttaccgat caggggatcg ccgtcaataa
    20401 cgtgatggcg ctgggcggca tcgcgcggaa aaaccaggtc attatgcagg cctgctgcga
    20461 cgtgctgaat cgcccgctgc aaattgttgc ctctgaccag tgctgtgcgc tcggtgcggc
    20521 gatttttgct gccgtcgccg cgaaagtgca cgcagacatc ccatcagccc agcaaaaaat
    20581 ggccagtgcg gtagagaaaa ccctgcaacc gcgcagcgaa caggcacaac gctttgaaca
    20641 gctttatcgc cgctatcagc aatgggcgat gagcgccgaa caacactatc ttccaacttc
    20701 cgccccggca caggctgccc aggccgttgc gactctataa ggacacgata atgacgattt
    20761 ttgataatta tgaagtgtgg tttgtcattg gcagccagca tctgtatggc ccggaaaccc
    20821 tgcgtcaggt cacccaacat gccgagcacg ttgttaatgc gctgaatacg gaagcgaaac
    20881 tgccctgcaa actggtgttg aaaccgctgg gcaccacgcc ggatgaaatc accgctattt
    20941 gccgcgacgc gaattacgac gatcgttgcg ctggtctggt ggtgtggctg cacaccttct
    21001 ccccggccaa aatgtggatc aacggcctga ccatgctcaa caaaccgttg ctgcaattcc
    21061 acacccagtt caacgcggcg ctgccgtggg acagtatcga tatggacttt atgaacctga
    21121 accagactgc acatggcggt cgcgagttcg gcttcattgg cgcgcgtatg cgtcagcaac
    21181 atgccgtcgt taccggtcac tggcaggata aacaagccca tgagcgtatc ggctcctgga
    21241 tgcgtcaggc ggtctctaaa caggataccc gtcatctgaa agtctgccga tttggcgata
    21301 acatgcgtga agtggcggtc accgatggcg ataaagttgc cgcacagatc aagttcggtt
    21361 tctccgtcaa tacctaggcg gttggcgatc tggtgcaggt ggtgaactcc atcagcgacg
    21421 gcgatgttaa cgcgctggtc gatgagtacg aaagctgcta caccatgacg cctgccacac
    21481 aaatccacgg cgaaaaacga cagaacgtgc tggaagcggc gcgtattgag ctggggatga
    21541 agcgtttcct ggaacaaggt ggcttccacg cgttcaccac cacctttgaa gatttgcacg
    21601 gtctgaaaca gcttccaggt ctgcccgtac agcgtctgat gcagcagggt tacggctttg
    21661 cgggcgaagg cgactggaaa accgccgccc tgcttcgcat catgaaggtg atgtcaaccg
    21721 gtctgcaggg cggcacctcc tttatggagg actacaccta tcacttcgag aaaggtaatg
    21781 acctggtgct cggctcccat atgctggaag tctgcccgtc gattgccgta gaagagaaac
    21841 cgatcctcga cgttcagcat ctcggtattg gtggtaagga cgatcctgcc cgactgatct
    21901 tcaataccca aaccggtcca gcgattgtcg ccagcctgat tgatctcggc gatcgttacc
    21961 gtctgctggt taactgtatc gacacggtga aaacaccgca ctccctgccg aaactgccgg
    22021 tggcgaatgc actgtggaaa gcgcaaccgg atctgccaac tgcttccgaa gcgtggatcc
    22081 tcgctggtgg cgcgcaccat accgtcttca gccatgcgct gaacctcaac gatatgcgcc
    22141 agttcgccga gatgcacgac attgaaatca cggtgattga taacgacacc cgcctgccag
    22201 cgtttaaaga cgcactgcgc tggaacgaag tgtattacgg atttagtcgc taagtagcca
    22261 catcaggtat gtaacgcctg atgcgacgct gactcgtctt atctggccta cacgctgcga
    22321 ttttgtaggc cggataagca aagcgcatcc ggcattcaac gcctgatgcg acgctggcgc
    22381 gtcttatcag gcctacgcgc tgcgattttg tagaccggat aagcgaagcg catccggcac
    22441 gaaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa cctggcgctg
    22501 ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgacgttga tcgcgagcgc
    22561 ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc tgacgatatg
    22621 gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc ctcctccgac
    22681 acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat tgtgcatacg
    22741 cactcgcgcc acgccaccat ctgggcgcag gcgggtcagt cgattccagc aaccggcacc
    22801 acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac cgacgcagaa
    22861 atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt tgaaaaacag
    22921 ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc gtttgcatgg
    22981 ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt cgcttatatg
    23041 gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac gctgctggat
    23101 aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg actgtataaa
    23161 accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga tggattttca
    23221 gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg caagggacag
    23281 aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt gcaccgcaag
    23341 agtccgtggc gtttattcac gccgatcagg ttccccgcgc tcagcatatt ttgcagggtg
    23401 aacaaggctt tcgtctgaca ccgctggcgt taaaggattt tcaccgccag ccggtgtatg
    23461 gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg cgtgaaggtg
    23521 gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg gagcggttta
    23581 tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt aatgcccgtc
    23641 tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat attgaaacca
    23701 cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc atcgtttata
    23761 tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa tacgtcgcca
    23821 gccgcccgct gctgctggaa aaactcaacg cctggtttgc aaaccacgat cccgatgtga
    23881 tcatcggctg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat gccgagcgtt
    23941 accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc gagcacggct
    24001 ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac ggtatcgagg
    24061 cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc gctcaggagc
    24121 tattaggcga aggaaaatct atcgataacc cgtgggttcg aatggacgaa attgaccgcc
    24181 gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc gagctggtga
    24241 cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca acggtgaacg
    24301 gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc tattttccgc
    24361 gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg cacgccagcc
    24421 ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg gtgctggact
    24481 ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc gggctggttg
    24541 aaggcatggc gcaacctgat ccagagcaca gtaccgaagg ttttctcgat gcctggttct
    24601 cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg cgcgatgaag
    24661 ccaaacgtca gggtaataag ccgctttcgc aggcgctgaa gatcattatg aatgcctttt
    24721 atggcgtgct cggcaccacc gcctgccgct tcttcgatcc gcgcctggca tcgtcgatca
    24781 ccatgcgtgg tcatcagatc atgcggcaga ccaaagcatt gattgaagcg cagggctacg
    24841 acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca cattcggaag
    24901 aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg tgggcggaaa
    24961 cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact catttctgcc
    25021 gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc tacgccgggc
    25081 tgattcagga gggcgacaag cagcggatgg tgtttaaagg gctggaaacc gtgcgcaccg
    25141 actggacgcc gctggcccag cagtttcagc aggagctgta cctgcgtatc ttccgcaacg
    25201 agccatatca ggagtacgtc cgccaaacca tcgacaaact gatggcgggt gaactggatg
    25261 cgcggctggt ttaccgtaaa cgccttcgcc gtccgctgag cgaatatcag cgtaatgttc
    25321 cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa tcaaaagcgc ggtcgcccct
    25381 tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc ccggagccgc
    25441 tggactacca acgttcacca ctggattacg aacactatct gacccgccag ctacaacccg
    25501 tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg accgggcaac
    25561 ttgggctatt ttgagcaaaa aaaagagttc gccagatacc attttgatgc gtgacgaatg
    25621 ctttgccatc cagtaccata gcgccctttc cattcctgga cctgaataac accactacct
    25681 cataagcacg gtagcgggtg gttattgcct gcaattaaag atatagagcc gaacacatat
    25741 gccttttaca cttggtcaac gctggatcag cgatacagaa agcgaattgg gacttggaac
    25801 cgttgtcgcg gtggatgcgc gaactgtcac tttacttttc ccatctactg gtgaaaaccg
    25861 tctgtacgca cgcagtgatt cccccgtgac ccgcgtgatg ttcaaccctg gtgataccat
    25921 taccagccat gacggctggc agatgcaagt cgaagaagta aaagaagaaa atggcttgct
    25981 gacctatatc ggtactcgcc tggatactga agagtccggc gtagccctgc gtgaagtttt
    26041 ccttgatagc aaactggtgt tcagcaaacc gcaggaccgt ctgtttgccg ggcagattga
    26101 ccgtatggac cgctttgcgc tgcgttatcg cgcgcgtaag tattccagcg aacagttccg
    26161 tatgccgtac agcggcctgc gcggtcagcg taccagcctg atcccgcatc agctcaacat
    26221 cgctcatgat gttggtcgcc gccacgcgcc gcgcgtcctg ctggctgacg aagtgggttt
    26281 agggaaaacc attgaagccg ggatgatcct gcatcagcaa ctgctctctg gcgctgctga
    26341 acgtgtgctg attatcgtcc cggaaacctt acagcatcag tggctggtag aaatgctgcg
    26401 ccgtttcaac ctgcgctttg ctctgtttga tgatgagcgt tatgccgaag ctcagcacga
    26461 tgcttacaac ccgtttgaca ccgaacagct ggtgatttgc tcgctggatt ttgcccgtcg
    26521 tagcaaacag cgcctggaac atctctgtga agccgaatgg gacctgctgg tggtcgatga
    26581 agcgcatcac ctggtgtgga gcgaagatgc gccaagccgt gaatatcagg ccattgaaca
    26641 actggcagag cacgtgccgg gcgttctgct gctgaccgcg accccggaac agctggggat
    26701 ggaaagccac ttcgcccgtc tgcgtctgct ggacccgaac cgtttccacg attttgcgca
    26761 gttcgttgaa gagcagaaaa attatcgtcc ggttgcggac gccgttgcca tgctgctggc
    26821 aggtaacaaa ctgagcaatg acgaactgaa catgctcggc gagatgatcg gcgagcagga
    26881 tatcgagccg ctgttgcagg cagcaaacag cgacagcgaa gatgcccaga gcgcccgtca
    26941 ggagctggtt tcgatgctga tggatcgcca cggcaccagc cgcgtgctgt tccgtaacac
    27001 gcgtaacggt gtgaaaggat tcccgaaacg cgagctgcac accattaagc tgccgctacc
    27061 gacgcagtat cagacggcta ttaaagtctc cggcattatg ggcgcacgta aaagtgcgga
    27121 agatcgtgct cgcgatatgc tctacccgga gcgtatttat caggaatttg aaggtgataa
    27181 cgccacctgg tggaacttcg atccgcgcgt tgagtggctg atgggctacc tgaccagcca
    27241 tcgctctcag aaagtgctgg tgatctgcgc caaagctgcc actgcgctgc aactggagca
    27301 ggtactgcgc gaacgtgaag gtattcgcgc tgcggtgttc cacgaaggta tgtcgattat
    27361 cgaacgtgac cgcgctgccg cctggtttgc cgaagaagac accggcgcac aggtactgct
    27421 gtgctcagaa atcggttctg aaggacgtaa ctttcagttc gccagccaca tggtgatgtt
    27481 tgacctgcca ttcaacccgg atctactgga gcagcgtatt ggtcgtctgg atcgtatcgg
    27541 ccaggcgcac gatattcaga tccatgtgcc ttatctggag aaaaccgctc agtcggtgct
    27601 ggtgcgctgg tatcacgaag gtctggatgc atttgagcac acctgcccga ccggacgcac
    27661 tatttacgat agcgtataca acgatctgat taactatctg gcttcaccgg atcaaaccga
    27721 aggctttgac gatctgatca aaagctgccg cgagcaacat gaagcgctga aagcacagct
    27781 ggaacagggt cgtgaccgcc tgctggaaat ccactccaac ggtggcgaaa aagcccaggc
    27841 actggcagaa agcattgaag agcaggatga cgataccaac ctgatcgcct tcgccatgaa
    27901 cctgttcgat attatcggta tcaatcagga cgatcgcggc gacaacatga tcgtgctgac
    27961 gccgtccgat catatgctgg tgccggactt ccctggcctg tcggaagatg gcatcaccat
    28021 cacctttgat cgtgaagtgg cgctggcgcg tgaagatgca cagtttatta cctgggagca
    28081 tccgctgatc cgcaacggtc tggatctgat cctttctggc gataccggta gcagcacgat
    28141 ttcactgtta aaaaacaaag cgttgccggt aggtacgctg ttggtggaac tgatttatgt
    28201 ggttgaagcc caggctccga agcagttgca gctcaaccgc ttcctgccac cgacgccggt
    28261 acgtatgctg ctggataaaa acggcaacaa cctggcggcg caggtagagt ttgaaacctt
    28321 taaccgccag cttaacgcgg ttaaccgtca caccggcagc aaactggtta acaccgtgca
    28381 gcaggatgtt cacgctatcc ttcaactggg tgaagcgcag atcgagaaat ctgcccgtgc
    28441 attgattgat gcagcgcgta acgaagccga cgaaaaactg tctgccgagc tgtctcgtct
    28501 ggaagctctg cgtgcagtga acccgaacat tcgtgacgac gaactgaccg ccattgagag
    28561 caaccgtcag caggtaatgg aaagcctgga tcaggcaggt tggcgtctgg atgccctgcg
    28621 tttgatcgtt gtaacgcatc agtaacggag ccgaaaatgg ggatggaaaa ctacaatcca
    28681 ccgcaggaac cctggttggt tatcctgtat caggatgacc atattatggt ggtcaacaag
    28741 ccgagcggtt tgttgtcagt gccgggtcgt ctggaagagc acaaagacag cgtgatgacg
    28801 cgcattcagc gtgattatcc gcaggcagaa tcggtgcatc gtctggatat ggctaccagc
    28861 ggcgtgattg tagtggcgct gaccaaagcc gcggagcggg agttaaaacg ccagttccgc
    28921 gagcgcgagc cgaaaaagca gtatgtggcc cgcgtctggg ggcatccatc ccccgcagaa
    28981 ggtctggtgg atctgccgct gatttgcgac tggccaaacc gcccgaaaca gaaagtctgt
    29041 tacgaaacgg gtaaacctgc gcagacggaa tatgaagtgg tggagtatgc ggcggataac
    29101 acggcaagag cggtgttaaa accgattacc gggcgttcgc atcaattgcg tgtgcatatg
    29161 ctggcgctgg ggcatccgat tctcggcgat cgtttttatg catcaccaga agcgagagcg
    29221 atggcaccgc gtttgttgct acatgcagag atgttgacga ttacccatcc ggcgtatggc
    29281 aatagtatga cgtttaaagc gccagcggat ttttaatcgt cacgctggcg cagttcggta
    29341 ggcggataag gcgttcacgc cgcatccggc aattgtgcgc cgattgcctg atgcgacgcg
    29401 ggccgcgtct tatcaggcct acagtttaaa acttatttaa accctttctg ctgctttatc
    29461 agctcatatg cctgctgaat ttcctgcgct ttctgcttcg ccatctccat catctcaggc
    29521 ggcaaacctt tcgccaccag cttatcggga tggtgttcac tcatcagctt acggtaggca
    29581 cgtttgatgg tggtcgcatc atccgtcggc ttcacgccca gcacattaca ggcatcttcc
    29641 agcgtcgggc cacgctgcgc ttgctgccag ttaccaccgc cagattgctg ctgataaccg
    29701 ccgccaaact gtgcaccgcc ctgcatcatg cgcaaaaact ggtcaaactg ggcgcgggag
    29761 atccctaatt cttcagcaat gacatacagc accgcccgtt cattcgggtg cagtgaacca
    29821 tcagcaaacg ccgcctgaat ctggatctcc agaaacatac gaattaagtc aaaacgacca
    29881 aagcagacac tgcgaaactg gcgcatcttt tcgcgcagcg ggtaattatc tgattttccc
    29941 acccggaacg cgttttgcgc cgcagtacgg gaagcgccat gaaggttcat tcggtccatc
    30001 aactggctgg cgatatgaat atcagcctcc gtgacgcgac ctttggattt ggttaaatgc
    30061 cccatcactt caaaagtggt ggcaaaaaac agcgcctgac gctcacgctg gttggcgaac
    30121 cacgccattt tacggctacg ggctttatca aacatatggc caattaacag gcctaacact
    30181 acgccccaaa agccgccgcc catcagtaag gccacggcca cgccaatgat ttttccccaa
    30241 tactgcatat attccccaaa tcgacacacg gatatcaggg ctatctccca caatataaag
    30301 gtgcttttac cgttttccgg ctgcggtcaa ttgtgggaca tcgcctataa tttgcattat
    30361 catacctgtc attcaatgcc gtgcctaaca ccactgacgc tattcggaca ggattaacac
    30421 tagcgtagag atgacgagta cgttagtctc tgaccgtttg tcacgcgcaa cgttaccgat
    30481 gatggaacaa taaaatcaac gtatgaaaaa acgtatcccc actctcctgg ccaccatgat
    30541 tgccaccgcc ctttatagtc aacagggact ggcagccgac ctcgcctcac agtgcatgtt
    30601 gggcgtgcca agctatgacc gtcctctggt acagggcgat accaatgact tacccgtgac
    30661 tatcaatgct gaccacgcga aaggggacta cccggatgac gccgtgttta ctggcagcgt
    30721 ggatatcatg cagggtaaca gccgtctgca ggccgacgaa gtgcagctcc atcaaaaaga
    30781 ggcaccagga caaccggagc cggtacgtac cgttgatgcg ctcggtaatg tccattacga
    30841 cgataaccag gtgatcctca aagggccgaa aggctgggcg aatctgaaca ccaaagatac
    30901 caacgtctgg gaaggtgatt accagatggt gggtcgccag ggtcgcggta aagcggacct
    30961 gatgaaacaa cgtggcgaaa accgctatac cattctggat aacggtagct ttacctcctg
    31021 tctgccgggt tctgacacct ggagcgtggt aggtagcgaa attattcatg accgcgaaga
    31081 acaagttgcg gagatctgga acgcccgctt taaggtgggt ccggtaccga tcttttatag
    31141 cccctatttg cagttgccgg tgggtgacaa acgtcgctct ggtttcttga tcccgaacgc
    31201 caagtacacc accaccaact actttgagtt ctacctgcca tattactgga acatcgcgcc
    31261 aaatatggat gccaccatca cgccgcatta tatgcatcgt cgtggcaaca tcatgtggga
    31321 gaacgaattc cgctacctct cccaggcggg cgctggcttg atggaactgg actatctgcc
    31381 ttcagataaa gtctatgaag atgaacaccc gaacgatgac agttcacgtc gttggttatt
    31441 ctactggaac cactccgggg tcatggatca ggtgtggcgt ttcaacgtcg actacaccaa
    31501 ggtcagcgat cctagctact tcaatgattt cgataacaag tacggttcca gtactgacgg
    31561 ctacgcaacg caaaaattca gcgttggcta tgcggtgcaa aacttcaatg ccaccgtttc
    31621 aaccaagcag ttccaggttt tcagcgaaca gaacaccagt agctactcgg cagagccgca
    31681 gttagacgtt aattactacc agaatgatgt tggtccgttt gatacgcgta tttacggcca
    31741 ggcagtgcac tttgttaaca ccagagacga catgcctgaa gcaacccgtg ttcacctgga
    31801 accgaccatc aatttgccgc tctctaataa ctggggcagc atcaataccg aagcgaagtt
    31861 tctggcaacc cattatcagc aaaccaatct tgactggtat aactccagaa acacgaccaa
    31921 gctggacgaa tccgttaacc gcgtaatgcc gcaattcaaa gttgacggca aaatggtctt
    31981 tgaacgcgat atggaaatgc tggctccggg ttatacccaa acgctggaac cgcgcgcgca
    32041 gtatttgtac gtgccgtatc gcgatcagag cgacatctat aactacgact cgtctctgct
    32101 gcaatctgac tactctggcc tgttccggga ccggacttac ggcggtcttg accgtattgc
    32161 ctccgctaac caggtgacga ccggtgtcac atctcgcata tatgatgatg ctgccgttga
    32221 acgttttaat atttccgttg gtcaaatcta ctatttcacg gagtctcgca ctggcgatga
    32281 caacataaca tgggagaatg acgacaaaac gggctcactg gtgtgggcag gcgacaccta
    32341 ctggcgtatc tccgagcgtt ggggattgcg tggcgggatt cagtacgata cacgtctgga
    32401 taacgtagcg accagtaact ccagcattga ataccgtcgg gatgaagacc gtctggtaca
    32461 gctgaattac cattacgcca gcccggaata tattcaggct acgctgccta agtactattc
    32521 cactgctgag caatataaga atggtatttc gcaggtaggt gctgtcgcca gcaggccaat
    32581 tgccgatcgt tggtccattg ttggggccta ctactacgac accaatgcga acaagcaagc
    32641 cgactctatg ttaggtgtgc aatacagctc ctgctgctat gcaattcgcg tcggttacga
    32701 gcggaagctg aacggttggg ataacgataa acaacatgcg gtatatgaca acgcaatcgg
    32761 ctttaacatc gaacttcgcg gcctgagctc caactacggt ctgggtacgc aagagatgct
    32821 gcgttcgaac attctgccgt atcaaaacac tttgtgattt gttgatttac cacgtaatcc
    32881 gcagtgcggt taattgaaat ggaaaaagta tgaagaactg gaaaacgctg cttctcggta
    32941 tcgccatgat cgcgaatacc agtttcgctg ccccccaggt agtcgataaa gtcgcagccg
    33001 tcgtcaataa cggcgtcgtg ctggaaagcg acgttgatgg attaatgcag tcggtaaaac
    33061 tgaacgctgc gcaggcaagg cagcagcttc ctgatgacgc gacgctgcgc caccaaatca
    33121 tggaacgttt gatcatggat caaatcatcc tgcagatggg gcagaaaatg ggagtgaaaa
    33181 tctccgatga gcagctggat caggcgattg ctaacatcgc gaaacagaac aacatgacgc
    33241 tggatcagat gcgcagccgt ctggcttacg atggtctgaa ctacaacact tatcgtaacc
    33301 agatccgcaa agagatgatt atctctgaag tgcgtaacaa cgaggtgcgt cgtcgcatca
    33361 ccatcctgcc gcaggaagtc gaatccctgg cgcagcaggt gggtaaccaa aacgacgcca
    33421 gcactgagct gaacctgagc cacatcctga tcccgctgcc ggaaaacccg acctctgatc
    33481 aggtgaacga agcggaaagc caggcgcgcg ccattgtcga tcaggcgcgt aacggcgctg
    33541 atttcggtaa gctggcgatt gctcattctg ccgaccagca ggcgctgaac ggcggccaga
    33601 tggggtgggg ccgtattcag gagttgccgg ggatcttcgc ccaggcatta agcaccgcga
    33661 agaaaggcga cattgttggc ccgattcgtt ccggcgttgg cttccatatt ctgaaagtta
    33721 acgacctgcg cggcgaaagc aaaaatatct cggtgaccga agttcatgct cgccatattc
    33781 tgctgaaacc gtcgccgatc atgactgacg aacaggcccg cgtgaaactg gagcaaattg
    33841 ctgctgatat caagagtggt aaaacgactt ttgctgccgc agcgaaagag ttctcgcagg
    33901 atccaggttc tgctaaccag ggcggtgatc tcggctgggc tacaccagat attttcgatc
    33961 cagccttccg tgacgccctg acccgcctga acaaaggtca aatgagtgca ccggttcact
    34021 cttcattcgg ctggcattta atcgaactgc tggatacccg taatgtcgat aaaaccgacg
    34081 ctgcgcagaa agatcgtgca taccgcatgc tgatgaaccg taagctctcg gaagaagcag
    34141 caagctggat gcaggaacaa cgtgccagcg cctacgttaa aatcctgagc aactaatggt
    34201 taaaacccaa cgtgttgtga tcactcccgg cgagcccgcc gggattggcc cggacttagt
    34261 tgtccagctt gcacagcgtg agtggccggt cgaactggtt gtttgtgccg atgccactct
    34321 ccttaccgac cgggcagcga tgctcggttt gccgctcacc ctccgccctt attcccccaa
    34381 ctcccctgca caaccgcaaa ctacgggcac attaacgcta cttcctgtcg cgctacgtga
    34441 atctgtcact gcggggcagt tagcgattga aaatgggcat tacgtggtgg agacgctggc
    34501 gcgagcgtgt gatggctgtc tgaacggtga atttgctgcg ctgatcacag gccccgtgca
    34561 taaaggcgtc attaacgacg caagcattcc gtttaccggt cataccgagt ttttcgaaga
    34621 gcgttcgcag gcgaaaaaag tggtgatgat gctggcgacc gaagaacttc gcgtggcgct
    34681 ggcaacgacg catttaccgc tgcgcgatat cgcagatgct atcacccctg cacttttgca
    34741 cgaagtgatt gctattttgc atcacgattt gcgtacaaaa tttggtatta ccgaaccgcg
    34801 cattctggtc tgcgggctga atccgcacgc gggcgaaggc ggtcatatgg gtacggaaga
    34861 gatagacacc attattccgg tgctcgacga gctgcgggcg caggggatga aactcaacgg
    34921 gccgctgcct gccgataccc tgtttcagcc gaaatatctc gataacgccg acgccgtgct
    34981 ggcgatgtac cacgatcagg gtcttcccgt gctaaaatac cagggcttcg ggcgcggtgt
    35041 gaacattacg ctgggcctgc cctttattcg cacatcagtg gaccacggca ccgcgcttga
    35101 actggcggga cgtggcgaag ccgatgtcgg cagttttatt acggcgctta atctcgccat
    35161 caaaatgatt gttaacaccc aatgaataat cgagtccacc agggccactt agcccgtaaa
    35221 cgcttcgggc aaaactttct caacgatcag ttcgtgatcg acagcattgt ttctgccatt
    35281 aacccgcaaa agggccaggc aatggtcgaa atcggccccg gtcttgcggc attgaccgag
    35341 ccggtcggcg aacgtctgga ccagctgacg gtcatcgaac ttgaccgcga tctggcggca
    35401 cgtctgcaaa cgcatccatt cttaggcccg aaactgacga tttatcagca ggatgcgatg
    35461 acctttaact ttggcgaact ggccgagaaa atgggtcagc cgctgcgtgt tttcggcaac
    35521 ctgccttata acatctccac gccgttgatg ttccatctgt ttagctatac tgatgccatt
    35581 gccgacatgc actttatgtt gcaaaaagag gtggtgaatc gtctggttgc aggaccgaac
    35641 agcaaagcgt atggtcgatt aagcgtcatg gcgcaatact attgcaacgt gatcccggta
    35701 ctggaagttc cgccgtcagc ctttacacca ccacccaaag tggattccgc cgtcgtgcgc
    35761 ctggtccctc atgcaacgat gcctcacccg gttaaagatg ttcgcgtgtt gagccgcatc
    35821 accaccgaag cctttaacca gcgtcgtaaa accattcgta acagcctcgg caacctgttt
    35881 agcgtcgagg tgttaaccgg aatggggatc gacccggcga tgcgagcgga aaatatctct
    35941 gtcgcgcaat attgccagat ggcgaactat ctggcggaga acgcgccttt gcaggagagt
    36001 taacgatgat caattcgccc cgagtgtgta ttcaggttca aagcgtctac attgaggcac
    36061 aatcttcacc tgataatgaa cgttacgttt ttgcttatac cgtaaccata cgcaatctgg
    36121 ggcgagcgcc agtgcagttg ttggggcgtt actggctgat caccaatggc aatggccgtg
    36181 aaaccgaagt ccagggcgaa ggagtggttg gcgtccagcc acttatcgcg cctggcgaag
    36241 agtaccagta caccagcggt gcaatcattg aaaccccgct gggcaccatg cagggtcact
    36301 acgaaatgat cgatgaaaat ggcgtccctt tcagcatcga cattcccgta ttccgactcg
    36361 ccgttcccac actcattcat taaaagaata tggcgacata ccttattggc gacgttcatg
    36421 gttgttacga tgaactgatc gcattgctgc ataaagtaga atttaccccc gggaaagata
    36481 ccctctggct gacgggcgat ctggtcgcgc gcggcccggg ttcgctggat gttctgcgct
    36541 atgtgaaatc cttaggcgac agcgtacgtc tggtgctggg caatcacgat ctgcatctgc
    36601 tggcggtatt tgccgggatc agccgcaata aaccgaaaga tcgcctgaca ccgctgctgg
    36661 aagcgccgga tgccgacgag ctgcttaact ggctgcgtcg ccagcctctg ctgcaaatcg
    36721 acgaagagaa aaagctggtg atggcccacg cagggatcac gccgcagtgg gatctgcaga
    36781 ccgccaaaga gtgcgcgcgc gatgtagaag cggtgctgtc gagtgactcc tatcccttct
    36841 ttcttgacgc tatgtacggc gatatgccaa ataactggtc accggaattg cgggggctgg
    36901 gaagactgcg ttttatcacc aacgccttta cccgtatgcg tttttgcttc ccgaacggtc
    36961 aactggatat gtacagcaaa gaatcgccgg aagaagcccc tgccccactg aaaccgtggt
    37021 ttgcgattcc tggccctgtc gctgaagaat acagcatcgc ctttggtcac tgggcatcgc
    37081 tggaaggcaa aggtatgccg gaaggtattt acgcgctgga taccggctgc tgctggggtg
    37141 ggtcattaac ctgcctgcgc tgggaagata aacagtattt tgtccagccg tcgaaccggc
    37201 ataaggattt gggcgaagcg gcggcgtctt aaacacagcc tgatacagga aggccggata
    37261 agacgcgacc ggcgtcgcat ccggcactag ccgtaaattc tatacaaact taccgccgct
    37321 ccagaatctc aaagcaatag ctgtgagagt tctgcgcatc ggcgtcgtgg aattcgctga
    37381 ataccgattc ccagtcatcc ggctcgtaat ccgggaaatg ggtgtcgcct tccacttctg
    37441 cgtcgatatg cgtcagatac agtttttgcg cttttggcag gaactgctca taaacgcgac
    37501 cgccgccaat caccatgatt tctggtacgt caccacacgc cgcaatggct tcatccaccg
    37561 atttcaccca cgttacgcga tcgtccgtac ccggttgact gctgaggata atatttttgc
    37621 gtcctggcaa cggacgaccg attgattccc aagtatggcg gcccataatc acgggtttat
    37681 ttaaggtgtt gcgtttaaac caggcgagat cggcaggcag gttccacggc atggcgtttt
    37741 ccatgccgat aacgcgatct accgctaacg ccgcaatcag actgatcatt gagatttccc
    37801 gataaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg gtgcgaggat
    37861 gatgttgagg aaaattttat attctgctgg cgagtccacg ctctccccct ggactcgccg
    37921 cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt cactctttat
    37981 taagatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt accttcttct
    38041 gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc ggtgatgatc
    38101 tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt gtcattctca
    38161 accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc ggcacgttct
    38221 cgcgcttcat acggccccag acctaaactt tccagcgcca gacgcccggt tttcagcgca
    38281 ccttcaaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg aatgtagtga
    38341 tcgacatcgc gggcgcgggc aataatctgc aaatgcggga aatgttcttt caccatctcc
    38401 gtcagttgca ggttggtttg cggatcgtca atagcgttaa tcagcacttc cgctttcgcc
    38461 gctccggcag attccagtaa atccatccgc gtggcatcgc cataaaacac tttcatacca
    38521 aatttacgca gcgtttcgat atggtccgga tcgtgatcga gcaccaccat tttcaccccg
    38581 ctggagagca gtaaacgtcc ggtaatctgt ccaaaacgac cgaatccggc gataatcacg
    38641 cgcggctgtt cttcatcgat ctcatcggct tcacgcgcct cctccgtaga agattgctca
    38701 aggcgattga ggatcaccag cagaatcggc gttgctgcca tcgacagcgc caccgccagg
    38761 gtcagcgatt tcgcccactc cggctccagc acattcgcca tctgcgccgc accaaatacc
    38821 acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accagcgacg ctgtttattt
    38881 ggcacctgca acggtcgggc aatcagccac agcatggcga ttttgatgat gaggaaaccg
    38941 agcagcaaaa tgacaatgcg caatgggttt tcaatcagcg tgccaaagtc tatcgacatg
    39001 ccaacaccga tgaaaaacag ccccaacagc aaacctttaa acggttcgat atcgctttcc
    39061 aacgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc gcccatcgcc
    39121 atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacaaggaa taacgccacg
    39181 gcactaaaca cttcccgcaa gccagagcgg gcaacaaaac gcagcgccgg acgcgtaaca
    39241 tagcgcccca gcaataccac cagtaccagc gcacccgcca cttttaacgc cgagagagca
    39301 aatgcgccca tcgtcgtcga ggcactgctc gccgccagta gcgggatcat cgccaccagc
    39361 gggattgccg cgatatcctg gaacagcagc actgcaaagg cactgcggcc catttgcgtc
    39421 accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgttgatga gagcgccagc
    39481 gtcataccga tcaattccgc gacctgccag cgcagcccaa gtaacataca gaacagcccc
    39541 agcaggccac cgcaaatcac catctgcaat gcgccaccgc cgaacactgc cgcacgcagc
    39601 ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag caccacccca
    39661 atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc ccacgggcca
    39721 ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg taccgcaatg
    39781 ggtacaatca gcgccgccga tccgaggtag atcagcgcct gaaccagcgt atgactatcc
    39841 atgatgggcc tcctgccatt ccagcagacg ttgcttatag tggcgcgcct gcccttcgag
    39901 ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc agttcagccc
    39961 gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg gatgcgcacc
    40021 aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat gtttgccatg
    40081 cagcgccgtg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat cgatccaaag
    40141 tttaagaagc ggaggaatgc tgtaccactg catcgggtgc tgccagacga tcagatcggc
    40201 gcgagagagc gcctcctgct cggcggcaat atcgatattg aagtcaggat agagttgata
    40261 aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc gtttattcgc
    40321 atgggaatga tgcggatacg gatgcgcata aatgatgagg atcatagtta ttagcccgcg
    40381 tgtttacgcc tttgttttcc cacaaagtgt agtcatatat tcacgcggct tatagtcaat
    40441 aatattgatt cagaaaagag agaaatctga acaaataatc ccattctctg cccgcggatc
    40501 gtcatattgc tggtctgcgc cagtgacatt cctcgagtct ccggggcaaa cgctacggaa
    40561 atcaacaagc caaacagcga gatacccgcc cccattagca tcgtgttact gataccgtaa
    40621 ttattgataa atatcggcag tgcccaggtc gaaacaatgg tgccaatacg acttaaggac
    40681 ataatcacgc ccacggcaga ggcgcggata tccgtcggga agagttcatt aggatagagc
    40741 cactgcaaat tacccggccc gccagagaaa aaggcataca ccgcaaaggc catcactacc
    40801 agccagatcc ccatatccgg gattagcccc aaaaccgcca gcgccagcgt catcatggca
    40861 aagctgccaa tcaacaatgg acgccgtccg gcagtgttta gccacagcat cggcggaata
    40921 cagccgagca taaagaacag gctaatcacc acattcccta gtgccgcgtt tttgccaacc
    40981 cccaatccca acgaaccaac gatttgcggg ccaaaggtgt aaatggcgaa cattgggatc
    41041 acctggcagg tccagatggc ggcaacaaac agaacaaaag gaaaatggcg gcgattaaac
    41101 agatcgcgaa aacgggtttg ctgcggctgc tcttcatcga aagccaccgg ttcgccaaac
    41161 agttttatca tcatctcttc gcactctttt actcgccctt tgcgtaataa ccagcgggga
    41221 gattctggca gttcgaatcg accaatcaaa atcaacaaac aagggatcgc cgcgctaccc
    41281 agcatccagc gccaaccacc ttccacatca taaagccagt agccgaccag atcggcgcag
    41341 gtcgcgccga cataccacat cgcggcaata aagctgatgg aaaacgcccg ctgacgggta
    41401 ctggagaact cggtgatcat cgaagtggcg atgggataat ctgcaccgat gacaatgccg
    41461 atgagtaccc gcatcaccaa cagttcgacg ggggatgaaa cacacatcgt cgccaccgat
    41521 atcacgccga tggcgatgat atcaatgagg aacattttgc gccgtccgac tttatcggag
    41581 atatagccaa acagcgatgt gccaacgaac agcccggcga gcgttcccgc gcccagcaag
    41641 ccaatccagt cagcgtccag tttcagcgcc ggagtcagtt gctccagcgc cacgccaatc
    41701 attaccagta cataaccatc cagaaacgga ccaccgcttc cccacagcaa aatgcggcgg
    41761 tgaatagagg agaatttgag atcgtcaaag tttctggacg gttgcatggg ttattcctgt
    41821 ttttttgcaa tgagagtcag tacggcgtta cgtatcaaac caacatcagc cgtaacggaa
    41881 ctccacacca aaggtgccgc gcgggtattc ccactgttcc agcgccgacc ccagccccag
    41941 aatgcgacag gtgccgcact ccagacatcc ggcgtaatcg aagcgcacac tgccgtcatc
    42001 ctgcttcttg tacagacctg cggggcacgc tttcaccagc agctccagca cctgtttatc
    42061 agcatcggcc ttcacaacaa tgtgcggatg ctcttcatcg acattgaatt tattgacgcc
    42121 cagtttgacg tccacattga cgggagaagt cataaaacgg tcactccttt catgccatcc
    42181 ttgatcagat tgatgaagcc cactttcttg ccgtggcgga ggattttctt gcgcatcagt
    42241 tccggcgcac tgccgtcaat ggtgaacagg tcacgcgcca cgcccaccgc cagttccggg
    42301 tagccgctaa acatgcgtgg gttatcaagg aacgccggta gtttctggta catgcgcata
    42361 tcgcgcagcg ggctgctctc cagatgctga cgatattccg cgagtttttg cttactgaaa
    42421 tcgtcgcttt tcatcgctga aagcacggtt tttgctgcag cttccccggc ggcaatcgcc
    42481 agatccatac cgcgaatggt aaaaccgagg ttcatacaca ttccggcggc atcaccggca
    42541 atcaatacgc cgtcaccgac cagctccggc agcatgttga tgcctgcttc cggcactacg
    42601 tgagcggaat attccaccag cttgccgccc gcgatcagcg gtgcaacggc cggatgctgt
    42661 ttgaaatctt ccagcatttg cggcaccgat tttttcgcgt catgcagatg atgcagacca
    42721 caaaccagcc ccagcgacag ggtattttca ttggtataaa ggaagccgcc gcccatcagg
    42781 ccatcggtgg gtgatcccgc aaacagacaa gccgcgccct gattaccctg caactgaaaa
    42841 cggtcttcga ttaccgactt cggtgactcg atcagttcct tcacgccaac cgccacatcc
    42901 gtcggtttaa cgcgttttgc catccccaac ttttcggcaa ggatggagtt caccccatca
    42961 gcaaggagca ccgttttcgc ttcaatcaca tcgccatcgg cttctacacc gacgactttg
    43021 ccatcgcgct gtacgaggtt atcgacgcgg atcccggtaa ttaactgcgc gcccgcttct
    43081 tcggcctgct ccatcaacca ggcatcaaat ttactgcgca aaacggagta agaacgctgg
    43141 gatagcgagg tttcgtcacc gttgcagtag tccatagtca tcgctgactt ttccgtcata
    43201 aacgcgagtt tttcatgggt gatcaggcgt tctacggggg cgaaatcggc gaaacctgga
    43261 ataatgtgtt ccagactgtg ggcatagaga cgcccgccag taacgttctt ggcacccgcg
    43321 gaattgccac gctcgataac taacacttgc gccccttcgc gggcgagcac cagtgcggca
    43381 accgaaccgg caagccctgc accgacgatg atggcgtcaa agatatcttc ggacataaga
    43441 gctccctgtc agcggcagag ccgtaatgca gccctgccag agtggatcaa cgcgctaaag
    43501 ctgcggtcag tgccggaagg atcttcacgg catcgccaac aatgccgtaa tccgcgtact
    43561 ggaagatcgg cgcattttta tctttattga tggcgaaaat ggtttgcgac gcgttagcgc
    43621 caaccatgtg ctggatctgc ccggagatcc tcaccgccag gtacagttca ggtttcagca
    43681 tcaggttgga gataccgaca tagcgttcgt gctccatcca tttttcgttt tctgccaccg
    43741 gacgagaaca ggccaactcc gcacctatcg ccttgcaaag ctgttctgcc agtgcaatgt
    43801 tctctttgct gccaatgccg cgaccgacgc tgaccaccag acgggctttg tcgagatcga
    43861 cgctgttgct ctggcgcgtc tgggttgccg tgcgggtaat cgccacagcc ggagcctgcc
    43921 actccacggt gtgcgtttcg ccagtgcgtg acgcgtctgg ctgagccgca tcgaacgtgc
    43981 cgctgctgat ggtcagtacc gcatacggcg tggcaatgcg ttcttcgcca atcgccagac
    44041 cgccgtaaac catgtgtttc actgttgctt taccgtcctg tacgctgacg gtgctggcat
    44101 cgttagacac cgccgcttta aggcgataac ccagttttgc cgccagtaat ttgccgcgac
    44161 gggtgtttgg caacagcacc agaccgtcag cgccgtgctg gcgaatagtg tcagccatga
    44221 caccggcgta atcttcgatc atccgatcgt ccggtttgcc gtttaatttc cagacatgat
    44281 tagcgccgag ctggattgcc tgtgcgccgt cggcatcatt gaggacaaag gcattgattt
    44341 gattagctaa agcctgcgca ccgttcatca gttccggcag acgagagggg gtatcgctga
    44401 atacccagac ttgagaaaac gtgttcatag catcccctgt aattaaatga ctttgcgaag
    44461 attttcagca aatgcggcga tctgttcttc gccgtcgcct tcaatcacga tgcgctgacg
    44521 ctcgcgctgt ttcggcgcgg caacctgttg ttcagaccag gcgtccacgg cgttaaaacc
    44581 aatatccgcc gccgaccata cctggactgg ctttttcgcc gcgccgagaa tggctttcat
    44641 cgaaggaatt tgtggggagt tgatatcagt ggaaacagca acaaccgcag gcagcggaat
    44701 gcttaaggtt tcggtttcat cttccagttc gcgctcaacg gtgagggtat ctgccgtcag
    44761 ggagataatt ttgctgacgc cgttaactgc cggaatattg aggatttcgc ccaccagcag
    44821 accaacctgc tgggcataaa ggtcggaaga accatcgcca cagaggatca gatcaaagcc
    44881 tgctttctgg gcggctgcag ccagtgcgct cgccgtttgt tgcggcagtg cctgctcgaa
    44941 ctggtcatca atcaccacaa tcagttcatc cgggccgcgc gatagcacat ctttacgccc
    45001 tttggcgttg gtcagggctt taccgcccac acttaaggct gtcacctgcg cctctgctgc
    45061 ctgttgcttt agctggcaag ccgcttcaat agcgttgaga tcgtattggc ttattttggc
    45121 atcggctttg ctgaagtcta atgaaccatc agcattattg accgcaatat cctgttcatc
    45181 aggcacgcac ttatagcaag taataatctt cattgcatct ccagaaatca tgaaggaaac
    45241 gtcgttcata tcggtggtgt taattacaga tatcacgccc tttaatttgg aaataaaact
    45301 ttaatcacca atattgaaaa tgtcacacgc agataaatcc aactttcaat attgttaagt
    45361 tcctcaccaa tattgaaaac atggcgtagc aaaaagaaat tttcaatatt gttttataga
    45421 tcaccaatat tgaaagtcag atgctatttt ttacccacaa aagaacataa caaactgatt
    45481 aataaccaaa attaacgaaa aacatcctct attcactcga aacaaaaatg tgacaccaat
    45541 cacagaatac agcttattga atacccatta tgagttagcc attaacgcgt ccacgaggtt
    45601 aataataatt atattaaatg ttaacaaaaa taaaacaaac gggaacgcaa aataaatatt
    45661 cgttttcaca gtggaattaa ctcatgaaga atgaaaagag aaaaacggga atagaaccga
    45721 aggttttctt tccgccatta ataatcgtcg gcatactttg ttggcttaca gtcagagatc
    45781 tggatgcggc gaatgtcgtt attaatgctg tattcagtta cgtcaccaat gtatggggat
    45841 gggcatttga atggtatatg gtggtgatgc ttttcggttg gttctggctg gtgtttggcc
    45901 cgtatgccaa aaagcgttta ggtaacgaac cgccagaatt tagcaccgcc agttggatct
    45961 ttatgatgtt cgcctcctgt acgtctgctg ccgtactgtt ctggggatcg attgagatct
    46021 actactacat ctccaccccg ccgtttggct tagaaccgaa ctcgacagga gcgaaagagt
    46081 tggggctggc ttacagcttg ttccactggg gacctctgcc gtgggccact tacagcttcc
    46141 tttcagtcgc cttcgcttac ttcttctttg tccgcaaaat ggaagtgatt cgccccagct
    46201 cgacactggt gccgctggta ggtgaaaaac acgccaaagg gttgttcggt accatcgtcg
    46261 acaacttcta tctcgtcgcc ttgatcttcg cgatgggtac cagtctgggc cttgccacgc
    46321 cgctggtgac cgagtgtatg caatggttgt ttggcattcc gcataccctg caactggacg
    46381 ctatcatcat tacctgctgg attatcctca acgccatttg cgtcgcttgc ggtctgcaaa
    46441 aaggggtacg tatcgccagt gacgtgcgta gttacctgag cttcctgatg ctgggttggg
    46501 tgttcatcgt cagcggtgcc agcttcatca tgaactactt caccgattcg gtggggatgt
    46561 tgctgatgta tctgccgcgc atgttgttct ataccgatcc catcgctaaa ggcggcttcc
    46621 cgcagggctg gaccgtgttc tactgggcat ggtgggtgat ttacgccatc cagatgagta
    46681 tcttcctcgc ccgcatctcc cgtggtcgta ctgtgcgtga actgtgcttc ggcatggtga
    46741 tggggctgac agcatccacc tggatcctgt ggaccgtgct cggtagtaac actctgctgt
    46801 tgatggataa aaacatcatc aacattccaa atctgatcga acagtacggt gtggcgcgcg
    46861 ccatcatcga aacctgggcc gctctgccac tcagcaccgc caccatgtgg ggcttcttca
    46921 tcctctgctt tattgccacc gttacgctgg ttaacgcctg ctcttatacc ctggcgatgt
    46981 ctacttgccg cgaagtacgc gatggtgaag aaccaccgct gctggtgcgt atcggttggt
    47041 caattctggt tggcattatc ggtattgttc tgctggcgct cggcggcctg aaaccgattc
    47101 aaaccgccat tatcgccgga ggatgcccgc tgttcttcgt caacattatg gtgacgctct
    47161 cctttattaa agacgcgaaa cagaactgga aagattaatt aacccccaaa atatcaagag
    47221 gttgaaagat ggattttaat ttaaatgatg agcaggaact gtttgtcgcc ggtatccgcg
    47281 aactgatggc cagcgaaaac tgggaggcct attttgccga gtgcgaccgt gacagcgtct
    47341 acccggaacg ttttgtcaaa gcactggcgg atatgggtat cgacagtctg ctgatccctg
    47401 aagagcacgg tggtctggac gcggggtttg ttactctcgc cgccgtgtgg atggagctgg
    47461 gacgtctggg ggcaccaacc tatgtactgt accagctgcc gggcgggttc aataccttcc
    47521 tgcgcgaagg cacacaagag cagatcgaca agattatggc tttccgcggc accggtaagc
    47581 agatgtggaa ctcagcgatt accgaaccgg gcgcgggctc cgacgtgggt agcctgaaaa
    47641 cgacttatac ccgtagaaat ggtaagattt atcttaatgg tagtaagtgt tttattacca
    47701 gtagcgccta caccccgtac atcgtggtga tggcgcgcga cggggcttct ccggacaaac
    47761 ctgtctacac cgaatggttt gttgatatga gcaaaccggg catcaaagtg accaaacttg
    47821 agaagctcgg tctgcgtatg gatagctgct gtgaaatcac ctttgacgat gtggaactgg
    47881 acgagaaaga catgttcggt cgggaaggta acggctttaa ccgcgtcaaa gaagagttcg
    47941 accatgaacg tttcctggta gccctgacca actacggtac ggcgatgtgc gcctttgaag
    48001 atgcggcgcg ctacgccaac cagcgcgtgc agtttggcga ggctattggt cgtttccagt
    48061 tgattcagga aaaattcgcc cacatggcga tcaaattaaa ctccatgaaa aacatgctgt
    48121 atgaagcagc gtggaaagca gacaacggca ccatcacctc tggcgatgca gcgatgtgca
    48181 aatacttctg cgccaatgcg gcatttgaag tggtggatag cgcaatgcag gtgctgggcg
    48241 gtgtcgggat tgcgggcaac caccgcatca gccgcttctg gcgtgacctg cgtgtagacc
    48301 gcgtttccgg aggatctgac gaaatgcaga tcctgacgct gggtcgtgcg gtgctgaagc
    48361 aataccgcta agtccgtgcg ctgcctgatg cgacgctgac gcgtcttatc aggcctacgg
    48421 gagcacgaat gtaagccgga taaggcgttt acgccgcatc cggcagtcat gcgctacata
    48481 aatttttcag gagctaataa tggatcatct acccatgccg aaattcggac cgctggccgg
    48541 attgcgcgtt gtcttctccg gtatcgaaat cgccggaccg tttgccggac aaatgttcgc
    48601 agaatggggc gcggaagtta tttggatcga gaacgtcgcc tgggccgaca ccattcgcgt
    48661 tcaaccgaac tacccgcagc tctcccgccg caatttgcac gcgctgtcgt taaatatttt
    48721 caaagatgaa ggccgcgaag cgtttctgaa attaatggaa accaccgata tcttcatcga
    48781 agccagtaaa ggtccggcct ttgcccgtcg tggcattacc gatgaagtac tgtggcagca
    48841 caacccgaaa ctggttatcg ctcacctgtc cggttttggt cagtacggca ccgaggagta
    48901 caccaatctt ccggcctata acaccatcgc ccaggccttc agtggttacc tgattcagaa
    48961 cggtgatgtt gaccagccaa tgcctgcctt cccgtatacc gccgattact tttctggcct
    49021 gaccgccacc acggcggcgc tggcagcact gcataaagtg cgtgaaaccg gtaaaggcga
    49081 aagtatcgac atcgccatgt atgaagtgat gctgcgtatg ggccagtact tcatgatgga
    49141 ttacttcaac ggcggcgaaa tgtgcccgcg catgagcaaa ggtaaagatc cctactacgc
    49201 cggttgcggt ctgtataaat gtgccgacgg ctacatcgtg atggaactgg tgggcattac
    49261 ccaaattgaa gagtgcttta aagatattgg cctcgcacat ctactcggta cgccggaaat
    49321 cccggaaggc actcagctta tccaccgtat cgaatgccct tacggcccac tggtagaaga
    49381 aaaactcgat gcctggctgg cggcacatac catcgcagaa gttaaagaac gctttgccga
    49441 actgaatatc gcctgtgcca aagtactgac cgtaccggaa ctggaaagca atccacagta
    49501 tgttgcccgt gaatcaatca ctcagtggca aacgatggat ggtcgcacct gcaaagggcc
    49561 gaacatcatg ccgaaattca aaaataaccc cggacaaatc tggcgcggaa tgccctcaca
    49621 tggcatggac acggctgcca ttttgaaaaa tatcggctac agcgaaaacg acattcagga
    49681 gttggtcagc aaaggtctgg ccaaagttga ggactaaacg cgtggcgtca gtcggcttgc
    49741 ctgactagcg cccgcccaat aacaaacgaa caaaaatgga tagaggtgca atggatatca
    49801 ttggcggaca acatctacgt caaatgtggg acgatctggc ggacgtttac ggtcataaaa
    49861 cggcgctgat ttgtgaatcc agcggcggag tcgttaaccg gtatagttat cttgagttaa
    49921 atcaggagat taaccgcacg gcaaacctgt tttatacgct ggggattcgc aaaggcaaca
    49981 aggttgcact acatctcgac aactgcccgg aatttatctt ttgctggttc gggctggcaa
    50041 aaattggcgc gattatggtg ccgattaacg cccgcctgtt acgcgaggaa agcgagtgga
    50101 tcttgcaaaa tagccaggcg tgcctgctgg tgaccagtgc gcaattttat cctatgtatc
    50161 aacagattca gcaggaagat gccactcaat tgcggcacat ttgcctgaca gatgtggcac
    50221 ttcccgctga tgatggagtg agttcattta ctcaactgaa aaatcaacaa cctgccacct
    50281 tgtgctatgc accgccgcta tcgactgacg atacggcgga aattctcttc acctccggca
    50341 ccacctcccg accgaaaggt gtggtgatta cccattacaa cctgcgcttc gctggatatt
    50401 actccgcctg gcagtgtgca ctgcgtgacg atgacgtcta catgacggta atgcctgcgt
    50461 ttcatatcga ttgccagtgt actgcggcga tggcggcgtt ttctgccggg gccacctttg
    50521 tgctggtcga gaaatacagc gcccgcgcct tctggggaca ggcgcagaag taccgcgcca
    50581 ccattaccga atgtattccg atgatgatcc gtacgttgat ggtgcagccg ccttcagcga
    50641 acgatcggca acaccgcctg cgggaagtga tgttttatct caacttgtcg gagcaggaaa
    50701 aagacgcatt ttgtgaacgc ttcggcgttc gcttgctgac gtcttatggg atgacggaaa
    50761 ccattgtggg cattatcggc gatcgcccta gcgataaacg acgctggccg tcgattggtc
    50821 gggcggggtt ttgctacgaa gcggagatcc gcgacgatca caatcgcccg ctcccggcag
    50881 gtgagatcgg tgaaatctgt attaaaggcg taccagggaa aaccatcttc aaagagtatt
    50941 ttctcaaccc gaaagccact gcgaaagtgc tggaagccga cggctggcta cataccggcg
    51001 ataccggata ccgcgacgaa gaaggctttt tttatttcgt cgatcgccgc tgcaatatga
    51061 tcaaacgcgg tggcgagaat gtctcctgcg tggagctgga aaatatcatc gccacccatc
    51121 cgaaaattca ggacatcgtg gttgtgggta ttaaagattc gattcgcgat gaagccatca
    51181 aagcatttgt ggtgctgaat gaaggtgaaa cattgagcga agaggaattt tttcgcttct
    51241 gcgaacaaaa tatggcgaaa tttaaagtgc cctcttatct ggagatcaga aaagatctgc
    51301 cacgtaattg ctcggggaaa ataattagaa agaatctgaa ataaatgaaa cgtcagggaa
    51361 ctaccctgcc agctaataat cacgccctaa aacaatatgc attttttgca gggatgttgt
    51421 cgtccctaaa aaagcaaaaa tggagaaaag gaatgagtga atcattacat ctgacccgca
    51481 atggatcaat tctggaaatt acccttgatc gcccaaaagc gaatgctatt gatgcaaaaa
    51541 ccagctttga aatgggcgaa gtatttctaa atttccgtga cgatccgcaa ttacgtgtcg
    51601 ccattattac cggtgccgga gagaaattct tttccgcggg ctgggattta aaagcggcag
    51661 cagaaggcga agcaccggat gctgactttg gcccgggtgg ttttgcggga ttaaccgaaa
    51721 ttttcaatct cgacaaacca gttatcgcag ctgtgaacgg ctacgccttt ggcggcggtt
    51781 tcgaactggc gctggcggca gattttattg tttgtgccga taacgccagc ttcgccctgc
    51841 cggaagccaa actgggcatc gttcctgaca gcggcggtgt gctgcgtctg ccgaagatcc
    51901 tgccgcctac catcgtcaat gaaatggtga tgaccggcag acgaatgggc gcagaagagg
    51961 cgctgcgttg ggggatagtc aaccgcgtgg ttagccaggc ggaactgatg gataacgccc
    52021 gcgaactggc tcagcagctg gttaacagcg ccccgctggc gattgcggcg ctgaaagaga
    52081 tctaccgcac caccagcgaa atgccggtag aagaagcgta tcgctatatt cgcagcggcg
    52141 tgttgaaaca ctatccatcg gttctgcatt cggaagatgc cattgaaggg ccgctggcgt
    52201 ttgccgagaa gcgcgatccg gtgtggaaag gacgttaaca accgtgagct attacgcctt
    52261 tgaggggtta attccggtgg ttcacccgac ggcgtttgtc catcccagtg ccgtcttgat
    52321 tggcgatgtg attgtgggag ccggtgtcta catcggccca ctcgcctcac tgcgtggtga
    52381 ctacgggcgg ttgatcgtgc aagcgggagc caatattcag gatggctgca ttatgcatgg
    52441 ctactgcgac actgacacca tcgttgggga aaacggccat atcgggcatg gagcgatcct
    52501 gcatggttgt gtgattggtc gcgatgcatt ggtcgggatg aacagcgtaa ttatggatgg
    52561 cgcggtcatt ggcgaagaga gcattgttgc cgcaatgagc tttgtcaaag caggctttag
    52621 tggcgagaaa cgccagttgt tgatgggtac gccagcccgc gctgtacgca gtgttagtga
    52681 cgacgagtta cactggaaac ggttgaatac caaagagtat caggatcttg tcgggcgctg
    52741 tcatgcatcg ttacatgaaa cgcagccact gaggcaaatg gaggaaaatc gcccccgttt
    52801 gcaggggacg acggatgtga cgcctaaacg gtgatcttgc cggatgcgac ctggttgctg
    52861 gataagatgc tacaacatcg tatctggcaa gcaggacgcc cggtatcaac gagataaaat
    52921 taacgacgca tactctttga caacatcatc tgccacttct gctcgcggtt aagttccgtg
    52981 agcggaacgg caggaatacg cggtgtcttt cttacgccga ctaatttttc ccgactgtta
    53041 ttgcgcaggc gcgcgtaaat ttgctcatcg atatcgacca ctttaaccag tcgctgacac
    53101 tggcatcccc gcccttccag cttattaggg atcatcttaa cttcgcagct tatttctgtg
    53161 acttccgaca gaatataagt cagggtatta accgccttgc tgtgttcaat atcgaaatga
    53221 atagagatct ctcttgctat cacccaccga ttttcagcca tcatccattc ggcgattaac
    53281 aagtagagtg gtttttcaac atatccttca cacatacgaa ctccagtttc acaaattaaa
    53341 agtctggcta tgactcgttt tgaagcaatt aaacaagacc atattaaaat tgtggatata
    53401 tcgatagtat gcaacttcac cgtcgataag tgtgagctaa atcctgctta tgtcatcaaa
    53461 aatatcgatt ccccaaaaga tttacttaat gggcaaaaaa aacggtcctc atcagagaac
    53521 cgtattagtt attcgatcaa attagcggat gaaaaatatc cgccacgaca cgctattatt
    53581 tgatctgtgc gtgcatttcc tgcaccgaaa ttactttttc agtcgcatcg gcattcagcg
    53641 ccatcgcggt agcgaaacca ccgttcaggg tggtgtcata atgcacttta tattgcagcg
    53701 cactgcgacg gatcacgcgg gagtcttcaa tcgcacgacg gcctgaggtg gtgttgatga
    53761 tgtaggtata ttcgccattc ttgatacggt cctgaatgtg cggacggcct tcatgcacct
    53821 tgtttaccag acgcggattg atacccgctt cgcccagcac aatcgccgtg ccgtgggtcg
    53881 catccagctc gaagccctgt ttcagcagtt ttgccgccag gtccaccacg cgttctttat
    53941 cgccttcgcg cacggaaagc agcgcacgac cgtgtttctt catggtggag ttgctgccca
    54001 gctgcgcttt ggcgaacgct tcagcgaagg tgcggcccac gcccatgact tccccggtag
    54061 agcgcatttc tggccctaac agcgggtcaa cgcctgggaa tttattgaac ggcagcacca
    54121 cttctttcac cgagtagtac ggcggaataa cttctttggt tacgccctgc tcagccagcg
    54181 atttgccagc catcacacgc gccgccactt ttgccagcgg tacgccggtg gctttggaga
    54241 cgaacggaac agtacgcgcc gcacgcgggt ttacttcaat caggtaaact tcgttgtttt
    54301 tcaccgcaaa ctgcacgttc atcagaccgc gtacctgcaa ttcgaaggcc agtttctgca
    54361 cctgctggcg catcacatcc tgaatttcct gacttaaggt gtacgctggc agcgaacatg
    54421 cggagtcacc ggagtgcacg cccgcctgct cgatgtgctc catgatgccg ccaatcagca
    54481 ccatttcgcc gtcgcagatg gcatccacgt caacttctac tgcgtcatca aggaaatgat
    54541 ccagcaacac tggcgcatcg ttagacacgc taaccgccgt ctggaagtag cgacgcaggt
    54601 cagcttcgtc atagacgatt tccatcgccc gaccgccgag aacgtaagac ggacgcacca
    54661 ccagcgggta gccaatctct tttgccttct caactgccat ttcaatagca gtaacggtgg
    54721 cgttcgccgg ttgtttcagt ttcagacggt caaccgcatg ctggaagcgt tcacggtctt
    54781 ccgcacggtc gatagcatcc gggctggtgc cgataaccgg tacgccagcg gcttccagtg
    54841 cacgcgccag tttcagcggg gtttgaccgc cgtactgaac gataacgcct ttcggcttct
    54901 caatacgcac gatttccagc acatcttcca gagttaccgg ctcgaagtag aggcgatcgg
    54961 aagtgtcgta gtcggtagag acggtttccg ggttacagtt aaccataatg gtttcgtaac
    55021 cgtcttcacg cagcgccagc gaggcgtgta cgcagcagta gtcgaattcg ataccctgac
    55081 cgatacggtt tggaccgccg ccaagcacca tgattttttc acggtcggta gacggattcg
    55141 cttcgcactc ttcttcataa gtggagtaca tgtaagcggt gtcggtggca aactctgccg
    55201 cacaggtatc cacgcgcttg tagaccgggt gcaggtcata ttggtcacgc agcttacgga
    55261 tttccgcttc gcgtacgccc gccagttttg ccaagcgcgc atcggcaaag cctttgcgtt
    55321 tcagctggcg caggaattca gcgttcaggc cagtgatgcc cacttccgcc actttctctt
    55381 ccagacgcac cagttcttca atctgtacca ggaaccagcg gtcaatgtta gtcaggttga
    55441 agacgccgtc cacagacagg cccgcgcgga aagcatcggc gatgtaccag atacgctctg
    55501 cgcctgcgtc tttcagttcg cgacggattt tggttaacgc ttccgggtca tccaggctca
    55561 ctttcgggtc gaagccagtt gcgcccactt ccaggccgcg cagcgctttt tgcagggatt
    55621 cctgctgtgt gcgaccaatc gccatcactt cgccaaccga tttcatctga gtggtcagac
    55681 ggtcgttagc accggcgaat ttttcgaagt tgaagcgagg aattttagta accacatagt
    55741 cgatggacgg ctcgaaggag gccggagtac gtccgccagt gatgtcgttc atcagttcgt
    55801 ctagggtgta acccaccgcc agtttcgccg ccactttagc aatcgggaaa ccggtcgctt
    55861 tcgacgccag cgccgaagaa cgggacacgc gtgggttcat ttcgataaca atcaggcgac
    55921 cgtttttcgg gttcaccgca aactggacat tggaaccacc ggtttcaacg ccgatttcac
    55981 gcagcaccgc catcgaggcg ttacgcatga tttgatattc tttgtcagtc agcgtttggg
    56041 ctggcgcgac agtgatggag tcaccggtgt ggatgcccat cgcatcaaag ttttcgatag
    56101 agcagacgat gatgcagttg tcgtttttat cacgcaccac ttccatctcg tactctttcc
    56161 agccgatcag cgactcatca atcagcaact ctttggtcgg agagagatcc agaccgcggg
    56221 cgcaaatttc ttcaaactct tcacggttat aagcgatacc gccgccgcta ccgcccatgg
    56281 taaaggatgg gcgaataatg cacgggaagc ccacgtcagc ggcaacagcc agcgcttctt
    56341 ccatcgtgtg tgcgataccg gaacgcgcgg tttccagacc aattttcttc atcgctacgt
    56401 cgaaacgacg gcggtcttct gctttatcaa tcgcatcggc agtggcacca atcatggtga
    56461 caccgaactc ttccaacacg ccctgacgtt ccagctccag cgcgcagttc agcgccgtct
    56521 gaccgcccat ggttggcagc accgcgtccg ggcgctcttt ttcaataatc ttgcgtacca
    56581 cttcccagtg aatcggctcg atgtaggtcg catcggccat ttccgggtcg gtcatgatgg
    56641 tcgccgggtt ggagttcacc agaatgacgc ggtaaccctc ttcgcgcagg gctttacatg
    56701 cttgcgcgcc agagtagtca aactcacacg cctgaccgat aacaatcggg cccgcaccca
    56761 gaatcaggat actttttata tctgtacgtt ttggcatggc tcttttactc ctgattactt
    56821 agcggtttta cggtactgct caattaactt gataaagtgg tcgaacaacg gcgcggcgtc
    56881 gtgtggacca gggctggctt cagggtgacc ctggaagctg aacgccggtt tatcgatacg
    56941 atgaatgccc tgtaacgtac cgtcgaacag ggatttatgc gtgacacgca ggtttgcagg
    57001 taatgttgct tcatccaccg caaaaccgtg gttctgggcg gtgatcatca ccacgttttt
    57061 ctccacatct ttaaccggat ggttgccgcc gtggtgacca aatttcattt tgacagtctt
    57121 cgcaccgctc gccagcgcca gcagctgatg accgagacag atgccgaata ccggaatatc
    57181 ggtttcgagg aacttctgga tggcggtaat ggcgtaatcg cacggggccg ggtcgccagg
    57241 accgttggag aggaagatgc cgtctggatt cattttcagc acatcttccg cagaagtttg
    57301 cgccggaacg atggtcaggc gacagcctct atccaccagc atccgcagga tgttgcgctt
    57361 ggcaccaaaa tcataagcca cgacgtggaa cggcagctcg tcttcttttt tcgcttctgg
    57421 caggccgccg gtcaacgtcc agctcccttg tgtccagcta taggcttctg cggtggtcac
    57481 ttcttttgcc agatccatgc cgttcagacc tgggaacgcg cgggcttttt ctaacgccag
    57541 cgccgcatcc gggttatcgc ctgcgataat gcagccattc tgtgcgcctt tctcgcgcag
    57601 taaacgcgtc agcttacggg tatcgatatc ggcaatcgcc acgatgttat ggcgcttcag
    57661 gtaagaagag aggtcttcgg tattacggaa gttgctggca atcagcggca ggtcgcgaat
    57721 caccagacct tgtgcatgta cctgagaaga ttcttcatcg gcgtcattag tgccgacatt
    57781 gccaatatgg ggataagtaa gagtaacgat ttgacgagaa taggaaggat cagtgaggat
    57841 ttcttgataa ccggtcattg aagtattgaa aacgacttcc ccaaccgccg aacctgttgc
    57901 ccctatggcc cgaccgtgaa actgggttcc gtcttccaga accaatagcg ctgacttaat
    57961 caaaacaccc tccacagaat attcactcac tttatttgca tattaattca cagtgatgac
    58021 gtaaatcaat gcaaatctgc ttaccgacga atttctggca aacggcggca ttctggagat
    58081 atgggcgcta aaagtcaact taatggcagg attttttata ttattttgtg gctcatgcat
    58141 gttttacagg cattaagcga caaaaacatc atctaacctg ttctggaaaa cgcttgcgca
    58201 gcaagaagat tgcatgaaat aaaaacgagc aggaaaaagt ggaccaaatg gtcaaaattt
    58261 acattaaaac aacaaaaata gaagacgaaa catgtaattt ttggcagtta tagaaaattt
    58321 aaaagcataa caccaaaaat aaaagggcta aaaattaaag ccctttataa ttaataaatt
    58381 aaatgtgtta cttttgcatc ataataaaca ttttgtggtt acaaattatt gagatcaagc
    58441 acatctcgca tatcaaaaag accactttcc ttaccactca accacaaagc cgatcttacc
    58501 gcaccgttag caaatgtcat gcggctggac gccttatggg tgatctccag acgctcgcca
    58561 atatcggcaa acatcgcggt atgttcgcca acgatgtcac ctgcacgcac ggtggcaaaa
    58621 ccaatggtac caggcacacg ttcaccggta tggccttcac gactgtagac cgcgcaatct
    58681 ttcagatctt tatcaagggc gtgggcgatc gcctctccca ttgccagtgc ggtgcctgac
    58741 ggcgcatcaa ctttatgtct atgatgtgct tcaataattt cgatatcggt gtagtcaccc
    58801 atcactttgg ctgctttctc cagcagctta agcatgacgt taacgccaac gctaaaattg
    58861 gcggcaaaga caatcgcaat atcggcggcg gcgtcacgaa ttgcctgttt accggcttcg
    58921 tcaaaccccg tagtgccgat caccatccct ttgccatgct gccgacaaaa agcgagatgg
    58981 ttcagcgtac cttccggacg ggtaaaatcg ataaacacat caaaatcatc ttttatcgca
    59041 tcaagactgc tttgcacggt aacgcctgtt ttccctgcac cagccagctc accggcgtcg
    59101 ctgcccagta aagaagatcc ttcgcgctcc agcgccgcgc ccagctgcac gccttctaat
    59161 gccagtgccg cctgaatcaa ctggcggccc atacgccccc cggctcccgc gatggcaacg
    59221 cggatgtttg catcatgcat agctattctc ttttgttaat ttgcatagac cgttttcaga
    59281 gtaaccagcg catcgctacg ccgccagcca aaaacgctga taattagaaa ttaatgataa
    59341 acagggaggg atatcagtga aaggcatgag tcgatgaatg actgcatgcc gataacatgt
    59401 gacaggttac ggcaccagag ccagcacctc agccacccac tgttggaagc cttttacatt
    59461 cagatccaat gccacctgta cattggctgg tttgcccagg caaccgtcga tatcaaccac
    59521 cgtcgtgcct gaggtaaatt cgccttgagt ttctaccgcg acaaaacacg gcttgagggt
    59581 gaataagtcc gggcgcacca gccaggcgat ggcgcagaga tcgtgcattc gcagaccgct
    59641 ttgcatactg ccgctacggt agtggctaaa cagggcgtgg agcatttttc cggtgcgatt
    59701 caactccggt aaagtagcga gatagtcagg ggttaatatt gcctgattgg tgacatccag
    59761 accgcacatg acaatttcga tgccgctacg gaagacacag gcagccgctt ctggatcggc
    59821 agcgatatta aactcggcat ttggtgtaca gttgccgcga ccagcagaac cgcccatgat
    59881 caccagacga cgaatatacg gtttacattc cgggcattgt aaaagtaaca gcgcaatatt
    59941 ggttaacggg ccgatggcca ccagggtaac gggctctggt gcacgcatca gggcatcccg
    60001 aatcgccaga aacgccggta tcccgagtgg ctttcggttg tgctcaacaa agtcgtagcc
    60061 agccattccc gattcgccgt gcacagatgc tgcatcacgc ggtgcgcgta ccagcggcac
    60121 agcggctccc tgggcgagcg gaatctccgc attccagaaa tgcagcagtt gcagggcatt
    60181 gcgggtagtt ttctcaaccg agacattacc cgcgacggtg gtcatcagtt gcaggtcgag
    60241 ttcgggggca aaaatcgcgg cggcaatggc gacggcatcg tcaatgccgg ggtcagtatc
    60301 gaggaatata ggtaaacgca tgttttcttc ataaaaaatg ccggtaacaa gaccggcatt
    60361 ttcgcataac ttaggctgct aatgacttaa tcgacttcac gaatatcgac acgcagctct
    60421 ttcggcactt cgaaaacaat attttcttca cggccttcca gcggaatggc ttcaccacca
    60481 cccaactgct gcaaacgtgc caccacattc tgcaccagaa tatccggagc cgatgcgccc
    60541 gcagtcacgc cgacgcattt aacctctttc acccactctt cctggatatc tttcgcatcg
    60601 tcaatcaaaa acgcgtgttt gcccatacgc tgggccagct ccgccagacg gttggagttg
    60661 gaggagtttt tcgaaccgac caccaacaca acttccgcct gttctgccag ggcgcgtacc
    60721 gcttcctgac ggttagtcgt ggcgtagcag atgtcatctt tgcgcggacc gacaattttc
    60781 gggaagcgtt tacgcagcgc gtcgatcaca tcagacgtat catccaccga cagcgtggtt
    60841 tgggtcataa aggagagctt ctcttcgttt ttgaccgtca gtttccacac atcgtctggc
    60901 gattcgacca gatacattcc cccttcgggg ttgctgtact gccccatcgt cccttccact
    60961 tccgggtgcc cggcgtggcc gatgagaata gattcttcgc cacggcgact ggcgcgggca
    61021 acttccatat gcactttggt caccagcgga caggtggcat caaacaccgt caaatcgcgg
    61081 cttttcgctt cgttacgaac cgcctgagaa acaccgtgtg cggagaaaat caggatcgcg
    61141 ccgtccggca cttcgctaat ctgctcaata aagatagccc cacgctcgcg caagctatcg
    61201 accacgtagc ggttatgcac cacttcgtga cggacatata tcggtgcgcc gtaaatggcc
    61261 agcgcgtttt caacaatgct gatagcgcgg tctaccccgg cacaaaaacc acgcgggttg
    61321 gccaacagga tctgcatgtt acgcctccag tgccggatcg atttccagca cttcaatatc
    61381 aaaatgaacg gtctgcccgg ccagcggatg gttgaaatca acggtaatgg agtcgccgtt
    61441 aatttcgcgg atcacaccag gcatctcact gccatccatt gcggtaaaaa gcatgattgc
    61501 gccaatttct ggctcgcctg catccataaa ttcacggcgg gagaagtact gaatcaggtc
    61561 cggtgacggc acgccaaacg ccgcatctgg ctccagcgag aaggtggttt tgtcgcccac
    61621 tttcagcccc agcaggtgtt gctccagccc ttcagaaaga gaagcatcgc ccaggcggaa
    61681 cagcgccggt ttaccgttgt tgcgagtaga ctcggcggtg gtgccatcgt caagttttag
    61741 cgtgaagtgc accaggacgg cgctattgct ctgtacagat tcagacatgc aggtttgctc
    61801 ttatttttga ggatttttgc cggatgcggc ctacgatttc gtagcaatct gtaggccgca
    61861 tccggcaggg tttattattg ttttttcgct ttggaaggta aaaacccttc cagcacgatc
    61921 agtgccgcac cgacacaaat ggcagtatcg gcaaggttga aggtggcgaa gtgccagtcg
    61981 ccgacgtaga agtcgatcat atcgacaacg aagccgtgcc acaggcggtc gaacaggttg
    62041 cccagcgcgc cgccaataat cagcgcgtaa gcgatattgt ttagcttctg cgtggccttc
    62101 gagcgataca tcatcactgc caggagcacg ctaataccaa tcgcaatacc ggcaaagaac
    62161 caacgctgcc agccgccgct atcggcaagg aaactaaacg ccgcgccata gttacgcgca
    62221 taatgcagat taagcgacgg gaacagcggg accgtatccc ccagagcaaa gttctggagg
    62281 atcaggtatt tgctgcccag atcgataatc agcacgacta ccaccagcca cagccagcgt
    62341 agccctgttg aacagatcga ttgactcatc aggcaaactt acgtttttca ccgtcaccgg
    62401 cgacgttgct gacacagcgg ccgcagattt ctgcgtgttc cgccaccttg ccgacatcct
    62461 gggtgtagtg ccagcagcgt gggcacttct caccttcggc tttactcaac gcgactttca
    62521 gccctttgag tacttcgctc tgctgagcat cagcaggtgc gtcgttatag tctgcaacgg
    62581 tagcgccgga ggttaacagg acaaatcgta attcatcgcc cagcgcggtc agtttcgccg
    62641 ccagttccgg ttctgcatac aaggttactg ccgcttccag cgagccaccc actttcttgt
    62701 cggcacgcgc ttgctcaatg actttgttca cttcgccacg cactttcaac agctcgtccc
    62761 agaacgcatc gttcattgct tcactgtctg ccagaccaaa caggccttcg taccactcac
    62821 cggtgaagac gtatttttca cgttcgcccg gcagatagcc ccacacttca tcagcggtga
    62881 aggagaggat tggtgccatc cagcgcacca gcgcttcagc gatgtgatac agcgcagtct
    62941 ggcagctacg acgcgccacg ctatccgctt tggcggtgta ctgacggtct ttgatgatgt
    63001 cgaggtagaa ggaacccatc tcaacggagc agaagcgcat cagacgctgt accacttcgt
    63061 ggaaatcgta tgcttcgtac gccttgagga tgtcttcctg tgccgctttc gcacaaccta
    63121 cggcccagcg atccagtacc accatctctt ccggtttcac catatctttt gctggatcaa
    63181 aaccattcag gtttgccagc aggaagcgcg cggtgttacg gatacgacga tagctatcgg
    63241 cagcacgttt caggatctcg tcagaaacgg ccatttcacc ggtgtagtcg gttgatgcca
    63301 cccacagacg cagaatatcc gcgcccagtt tgttcatcac atcctgcggc gaaacggtgt
    63361 tgccgatgga tttagacatc ttgcgaccct gaccatccac ggtaaagccg tgggtcagta
    63421 cctgacgata cggcgctttg cctttcatcg cggtggagat cattagggaa gacatgaacc
    63481 aaccacggtg ctggtcagaa ccttccagat acatgtccgc tgcgtgaccg gcaaattccg
    63541 gacgcacgtc aacaacagaa gagtgggtag atccggagtc aaaccatacg tcgagagtat
    63601 ccggcacttt cacatactgg tcagcttcgt cgccgaggat ctctttcgca tcgagatccc
    63661 accacgcctg gatgccgtca acttcaacgc gtttagccac ttcttccatc agttcgaggg
    63721 tacgcggatg cagctcttcc gtgtctttgt gcacgaacag tgacatcggt acgccccagg
    63781 tgcgctgacg ggagatacac cagtcaggac ggttagcaac catcgactcg atacgcgcct
    63841 ggccccagtc cgggatccac tgcacgcctt tgatctcttt cagtgactgc gcacgcagac
    63901 ctttctgatc catgctgacg aaccactgcg gtgtcgcgcg gaagatgatc ggcgttttgt
    63961 gacgccagca gcacggatag ctgtgctgca ttttctcaac gtgcagcagc gcgccttttt
    64021 cctgcagcag cgcaacgacg atgtcgttcg ctttgaagac gttcacgcca tccagcgtcg
    64081 gataagtgcc cggcagataa gtgccgtccg ggccaaccgg gttagcggtt tccaggccgt
    64141 atttctgacc gatcacatag tcgtccgggc cgtggccagg cgcggtgtga acggcaccgg
    64201 taccggcatc cagggtaacg tgatcgccga ggattgccgg aacgtcgaag cccataaacg
    64261 gatgggtaaa gcgcagcagc tcaagctctg caccttttac cgtgccgaga atggtgtaat
    64321 cggtcacgcc gatacgctgc attacgcttt caaccagatc tttcgccaga atcgcggcct
    64381 gaccgtcgat ctgcaccagc gcatagtcga aatcaggtgc aatagagatt gcgcggttcg
    64441 caggcagagt ccacggcgta gtggtccaga tcaccagcga gattgggccg ttaacgttgc
    64501 ttacgccaaa tttcgctttc agcgcatcct gatcgaccgc ctggaaagcg acgtcaatgg
    64561 acggagaagt tttgtcgtaa tactcaactt ccgcttctgc cagtgcagaa cggcagtcta
    64621 cgcaccagtg caccggcttc gcgcctttgt gcaggtgacc attgccgatg attttgccca
    64681 gcgcgcggat gatgttggct tcagttttga agtccatagt cagatacggg tgcgaccagt
    64741 cgcccagcac gcccagacgg ataaagtctt tgcgttgacc gtcaacctgg gtcgccgcgt
    64801 attcgcggca cttggcgcgg aactcggcgg cggtgaattt ctcacccggc ttaccgtatt
    64861 cttgctctac tttcagctcg atcggcagac cgtggcagtc ccagccaggc acatacggcg
    64921 agtcataacc ggaaagccct ttggacttca cgataatgtc tttcagaatc ttgttaaccg
    64981 agtgaccaat atgaatgctg ccattcgcat aaggagggcc atcatgcaga atgaaggttt
    65041 ttttgccttt tttagccgca cggatgatgc cgtacagatc atcatcagtc caacgcgcca
    65101 gcattcccgg ttcgcgcttg gcgagatcgc cacgcatcgg gaaccctgtt tccggcaaat
    65161 tcagggttga tttatagtca ctcatcagat tctcggttcc gtgtttcggt ttgattacat
    65221 aacaggctta agccggtttt gttagcccaa aaaattcgcg ggcggttaat tcatcacgcg
    65281 caatctgcgc tttcagttcg tccagcgacg caaatcgctg ctcattgcgt atttttttac
    65341 gcagcactac ttgtatatgg cgaccgtaaa ggtccattgc aacatctaac aaatgcactt
    65401 ccagctgctg gcgaataccg gcaaccgttg ggcgtgttcc gatgtttgcc acgccgggta
    65461 acggcttttc accgaggccc agcacttcta ccgcataaac ccctttcacc ggggaaacct
    65521 gacggcgtag cggtacattc gccgtcggga aacctatagt gcgccctaat tcatcaccgt
    65581 ggactacacg cccggagatg gcaaacgggt gccccagtaa actttctgcc agagccagat
    65641 tgtcatcagc cagcgcctga cgcacggcgg tgctgctgat acgcacacca ccttcgcaaa
    65701 aagtttgcgt actggtgata tcgaagccgt attccatgcc agctttctgt aataacaaga
    65761 aatcgccttc acgaccagcg ccaaagcgga aatcatcacc tacggcaaga aattttacgc
    65821 gcaaatgctt caccaaaaga tcgctgatga aattttgcgc ggttaacgcc gcaaaacgcc
    65881 tgtcgaaacg cacgcacagc acgtaatcaa cgccacactc tgcaaggtaa cgcagttttt
    65941 cccgcagccg ggtcagtctt gccggggctt tatcggtagc aaacagttcc agtggttgag
    66001 gttcaaaaag catcaccatc accggtaagt tgcgcttgcg cccttcttcc tgcaagccct
    66061 gtaacagcgc gcgatgaccg cgatgcacgc cgtcgaaatt accaatagtc agcacacacc
    66121 cttcttgcgg ggcctggctg agattatgta tgccgcgtat cagcttcatg tctggctcaa
    66181 aacagtgaaa atcgtccgag tataccttgt acagcggtca aggttaaccg gcgattgagt
    66241 accgaaatca gaaagaagtg gcttttcatt gccatggcgc aaatcacggg aagaaactga
    66301 ccgcctgctg caatttttat cgcggaaaag ctgtattcac accccgcaag ctggtagaat
    66361 cctgcgccat cactacgtaa cgagtgccgg cacattaacg gcgcttattt gcacaaatcc
    66421 attgacaaaa gaaggctaaa agggcatatt cctcggcctt tgaattgtcc atatagaaca
    66481 catttgggag ttggaccttg gctaatatca aatcagctaa gaagcgcgcc attcagtctg
    66541 aaaaggctcg taagcacaac gcaagccgtc gctctatgat gcgtactttc atcaagaaag
    66601 tatacgcagc tatcgaagct ggcgacaaag ctgctgcaca gaaagcattt aacgaaatgc
    66661 aaccgatcgt ggaccgtcag gctgctaaag gtctgatcca caaaaacaaa gctgcacgtc
    66721 ataaggctaa cctgactgca cagatcaaca aactggctta atcgccaatt tgctgaagct
    66781 ttgtgaaaaa gcccgcgcaa gcgggttttt ttatgcctgc tgcttttgtg atgcgtgaga
    66841 gaacacactg ccggttgcca gatagtcaaa agagacatgc cgttgtacta tctgaactct
    66901 tcgtggctta agaatcgtag aaccatcggc tgatacattc tgaacaagaa tatctactca
    66961 atctccagac tcccacacaa agaatatgga tattgtgata cacattgagg tattaaactt
    67021 aagaccactc tatttttgaa ttaaattcct atatacttta accattcata aatatctcct
    67081 cattagacct
//
LOCUS       DADMEQ010000008        65526 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.008, whole genome shotgun sequence.
ACCESSION   DADMEQ010000008 DADMEQ010000000
VERSION     DADMEQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.008"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(127..606)
                     /gene="folK"
                     /locus_tag="JNZ05_000528"
     CDS             complement(127..606)
                     /gene="folK"
                     /locus_tag="JNZ05_000528"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAY8563877.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(603..2021)
                     /gene="pcnB"
                     /locus_tag="JNZ05_000529"
     CDS             complement(603..2021)
                     /gene="pcnB"
                     /locus_tag="JNZ05_000529"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HAY8563878.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2060..2986)
                     /gene="gluQRS"
                     /locus_tag="JNZ05_000530"
     CDS             complement(2060..2986)
                     /gene="gluQRS"
                     /locus_tag="JNZ05_000530"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706092.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HAY8563879.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLITALGSYLQGR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3023..3478)
                     /locus_tag="JNZ05_000531"
     CDS             complement(3023..3478)
                     /locus_tag="JNZ05_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     DksA"
                     /protein_id="HAY8563880.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3656..4360)
                     /gene="sfsA"
                     /locus_tag="JNZ05_000532"
     CDS             complement(3656..4360)
                     /gene="sfsA"
                     /locus_tag="JNZ05_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAY8563881.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4375..4905)
                     /gene="thpR"
                     /locus_tag="JNZ05_000533"
     CDS             complement(4375..4905)
                     /gene="thpR"
                     /locus_tag="JNZ05_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HAY8563882.1"
                     /translation="MSEPQRLFFAIDLPAETREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4979..7408
                     /gene="hrpB"
                     /locus_tag="JNZ05_000534"
     CDS             4979..7408
                     /gene="hrpB"
                     /locus_tag="JNZ05_000534"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HAY8563883.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAQLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPNDPAN
                     TAPTRRTKKYS"
     gene            7604..10138
                     /gene="mrcB"
                     /locus_tag="JNZ05_000535"
     CDS             7604..10138
                     /gene="mrcB"
                     /locus_tag="JNZ05_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HAY8563884.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10358..12601
                     /gene="fhuA"
                     /locus_tag="JNZ05_000536"
     CDS             10358..12601
                     /gene="fhuA"
                     /locus_tag="JNZ05_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HAY8563885.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12652..13449
                     /gene="fhuC"
                     /locus_tag="JNZ05_000537"
     CDS             12652..13449
                     /gene="fhuC"
                     /locus_tag="JNZ05_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HAY8563886.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13449..14339
                     /gene="fhuD"
                     /locus_tag="JNZ05_000538"
     CDS             13449..14339
                     /gene="fhuD"
                     /locus_tag="JNZ05_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HAY8563887.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14336..16318
                     /gene="fhuB"
                     /locus_tag="JNZ05_000539"
     CDS             14336..16318
                     /gene="fhuB"
                     /locus_tag="JNZ05_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HAY8563888.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVMLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLIMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16353..17633)
                     /gene="hemL"
                     /locus_tag="JNZ05_000540"
     CDS             complement(16353..17633)
                     /gene="hemL"
                     /locus_tag="JNZ05_000540"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HAY8563889.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17858..19279
                     /gene="clcA"
                     /locus_tag="JNZ05_000541"
     CDS             17858..19279
                     /gene="clcA"
                     /locus_tag="JNZ05_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HAY8563890.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LIGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19303..19368
                     /locus_tag="JNZ05_000542"
     CDS             19303..19368
                     /locus_tag="JNZ05_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8563891.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19361..19705
                     /gene="erpA"
                     /locus_tag="JNZ05_000543"
     CDS             19361..19705
                     /gene="erpA"
                     /locus_tag="JNZ05_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HAY8563892.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19752..20375)
                     /locus_tag="JNZ05_000544"
     CDS             complement(19752..20375)
                     /locus_tag="JNZ05_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HAY8563893.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREISMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20413..21213)
                     /gene="btuF"
                     /locus_tag="JNZ05_000545"
     CDS             complement(20413..21213)
                     /gene="btuF"
                     /locus_tag="JNZ05_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HAY8563894.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21206..21904)
                     /gene="mtnN"
                     /locus_tag="JNZ05_000546"
     CDS             complement(21206..21904)
                     /gene="mtnN"
                     /locus_tag="JNZ05_000546"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAY8563895.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPTGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21988..23505
                     /gene="dgt"
                     /locus_tag="JNZ05_000547"
     CDS             21988..23505
                     /gene="dgt"
                     /locus_tag="JNZ05_000547"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAY8563896.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23635..25059
                     /gene="degP"
                     /locus_tag="JNZ05_000548"
     CDS             23635..25059
                     /gene="degP"
                     /locus_tag="JNZ05_000548"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HAY8563897.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25214..26370
                     /locus_tag="JNZ05_000549"
                     /pseudo
     CDS             25214..26370
                     /locus_tag="JNZ05_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
     gene            complement(26459..26845)
                     /locus_tag="JNZ05_000550"
     CDS             complement(26459..26845)
                     /locus_tag="JNZ05_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HAY8563898.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27159..27983)
                     /gene="dapD"
                     /locus_tag="JNZ05_000551"
     CDS             complement(27159..27983)
                     /gene="dapD"
                     /locus_tag="JNZ05_000551"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HAY8563899.1"
                     /translation="MQQLQNIIETAFERRADITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAIIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28014..30686)
                     /gene="glnD"
                     /locus_tag="JNZ05_000552"
     CDS             complement(28014..30686)
                     /gene="glnD"
                     /locus_tag="JNZ05_000552"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HAY8563900.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWHIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30748..31542)
                     /gene="map"
                     /locus_tag="JNZ05_000553"
     CDS             complement(30748..31542)
                     /gene="map"
                     /locus_tag="JNZ05_000553"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAY8563901.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31910..32635
                     /gene="rpsB"
                     /locus_tag="JNZ05_000554"
     CDS             31910..32635
                     /gene="rpsB"
                     /locus_tag="JNZ05_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAY8563902.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32893..33744
                     /gene="tsf"
                     /locus_tag="JNZ05_000555"
     CDS             32893..33744
                     /gene="tsf"
                     /locus_tag="JNZ05_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAY8563903.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33891..34616
                     /gene="pyrH"
                     /locus_tag="JNZ05_000556"
     CDS             33891..34616
                     /gene="pyrH"
                     /locus_tag="JNZ05_000556"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAY8563904.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34908..35465
                     /gene="frr"
                     /locus_tag="JNZ05_000557"
     CDS             34908..35465
                     /gene="frr"
                     /locus_tag="JNZ05_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAY8563905.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35557..36753
                     /gene="ispC"
                     /locus_tag="JNZ05_000558"
     CDS             35557..36753
                     /gene="ispC"
                     /locus_tag="JNZ05_000558"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HAY8563906.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36942..37700
                     /gene="ispU"
                     /locus_tag="JNZ05_000559"
                     /gene_synonym="uppS"
     CDS             36942..37700
                     /gene="ispU"
                     /locus_tag="JNZ05_000559"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HAY8563907.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37713..38570
                     /gene="cdsA"
                     /locus_tag="JNZ05_000560"
     CDS             37713..38570
                     /gene="cdsA"
                     /locus_tag="JNZ05_000560"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAY8563908.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38582..39934
                     /gene="rseP"
                     /locus_tag="JNZ05_000561"
     CDS             38582..39934
                     /gene="rseP"
                     /locus_tag="JNZ05_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HAY8563909.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRVAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39964..42396
                     /gene="bamA"
                     /locus_tag="JNZ05_000562"
     CDS             39964..42396
                     /gene="bamA"
                     /locus_tag="JNZ05_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HAY8563910.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFREGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42518..43003
                     /gene="skp"
                     /locus_tag="JNZ05_000563"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42518..43003
                     /gene="skp"
                     /locus_tag="JNZ05_000563"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HAY8563911.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43007..44032
                     /gene="lpxD"
                     /locus_tag="JNZ05_000564"
     CDS             43007..44032
                     /gene="lpxD"
                     /locus_tag="JNZ05_000564"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HAY8563912.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44137..44592
                     /gene="fabZ"
                     /locus_tag="JNZ05_000565"
     CDS             44137..44592
                     /gene="fabZ"
                     /locus_tag="JNZ05_000565"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAY8563913.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44596..45384
                     /gene="lpxA"
                     /locus_tag="JNZ05_000566"
     CDS             44596..45384
                     /gene="lpxA"
                     /locus_tag="JNZ05_000566"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HAY8563914.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45384..46532
                     /gene="lpxB"
                     /locus_tag="JNZ05_000567"
     CDS             45384..46532
                     /gene="lpxB"
                     /locus_tag="JNZ05_000567"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HAY8563915.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46529..47125
                     /gene="rnhB"
                     /locus_tag="JNZ05_000568"
     CDS             46529..47125
                     /gene="rnhB"
                     /locus_tag="JNZ05_000568"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAY8563916.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47162..50644
                     /gene="dnaE"
                     /locus_tag="JNZ05_000569"
     CDS             47162..50644
                     /gene="dnaE"
                     /locus_tag="JNZ05_000569"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAY8563917.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50657..51616
                     /gene="accA"
                     /locus_tag="JNZ05_000570"
     CDS             50657..51616
                     /gene="accA"
                     /locus_tag="JNZ05_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAY8563918.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51715..53856
                     /gene="ldcC"
                     /locus_tag="JNZ05_000571"
     CDS             51715..53856
                     /gene="ldcC"
                     /locus_tag="JNZ05_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HAY8563919.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53913..54302
                     /locus_tag="JNZ05_000572"
     CDS             53913..54302
                     /locus_tag="JNZ05_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY8563920.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54367..55665
                     /gene="tilS"
                     /locus_tag="JNZ05_000573"
     CDS             54367..55665
                     /gene="tilS"
                     /locus_tag="JNZ05_000573"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAY8563921.1"
                     /translation="MTLTLNRPLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55714..55974)
                     /gene="rof"
                     /locus_tag="JNZ05_000574"
     CDS             complement(55714..55974)
                     /gene="rof"
                     /locus_tag="JNZ05_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HAY8563922.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55961..56161)
                     /locus_tag="JNZ05_000575"
     CDS             complement(55961..56161)
                     /locus_tag="JNZ05_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HAY8563923.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56327..56872
                     /locus_tag="JNZ05_000576"
     CDS             56327..56872
                     /locus_tag="JNZ05_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HAY8563924.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56869..57291
                     /gene="arfB"
                     /locus_tag="JNZ05_000577"
     CDS             56869..57291
                     /gene="arfB"
                     /locus_tag="JNZ05_000577"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY8563925.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     APSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57305..58015
                     /gene="nlpE"
                     /locus_tag="JNZ05_000578"
                     /gene_synonym="cutF"
     CDS             57305..58015
                     /gene="nlpE"
                     /locus_tag="JNZ05_000578"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HAY8563926.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58170..58994)
                     /locus_tag="JNZ05_000579"
     CDS             complement(58170..58994)
                     /locus_tag="JNZ05_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HAY8563927.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRSIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59047..60765)
                     /gene="proS"
                     /locus_tag="JNZ05_000580"
     CDS             complement(59047..60765)
                     /gene="proS"
                     /locus_tag="JNZ05_000580"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAY8563928.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60876..61583)
                     /gene="tsaA"
                     /locus_tag="JNZ05_000581"
     CDS             complement(60876..61583)
                     /gene="tsaA"
                     /locus_tag="JNZ05_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HAY8563929.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61580..61984)
                     /gene="rcsF"
                     /locus_tag="JNZ05_000582"
     CDS             complement(61580..61984)
                     /gene="rcsF"
                     /locus_tag="JNZ05_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HAY8563930.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62102..62917)
                     /gene="metQ"
                     /locus_tag="JNZ05_000583"
     CDS             complement(62102..62917)
                     /gene="metQ"
                     /locus_tag="JNZ05_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HAY8563931.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62957..63610)
                     /gene="metI"
                     /locus_tag="JNZ05_000584"
     CDS             complement(62957..63610)
                     /gene="metI"
                     /locus_tag="JNZ05_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HAY8563932.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63603..64634)
                     /gene="metN"
                     /locus_tag="JNZ05_000585"
     CDS             complement(63603..64634)
                     /gene="metN"
                     /locus_tag="JNZ05_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HAY8563933.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64822..65397
                     /gene="gmhB"
                     /locus_tag="JNZ05_000586"
     CDS             64822..65397
                     /gene="gmhB"
                     /locus_tag="JNZ05_000586"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAY8563934.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15480 a        16232 c        18059 g        15755 t
ORIGIN      
        1 gggattgata tttatctgcg ggaatttata ttatgtttct gcaaaaatgt ttaaactaaa
       61 atttttatta aggaaagaga ggatgtatga ttttcagaaa atatttatat tcgtaatata
      121 aataaattac catttgctta atttgtcaaa tgctcttgta tgtaagactt cacgcaacgt
      181 ctccccatca ggaaacgcca gctccggtgc aatctcaaac agcggccaca gcataaatcc
      241 acgattcttc atatcgtagt gcggaacggt caggcgttcg gtatttatca cttcattacc
      301 aaacagcatg atatcgagat ccagcgtgcg tggtccccag cgttcagctt tacgaacacg
      361 accctgttgc agttctatgc gctgtgtatg gttgagtaac tcttccgggg cgggcgaagt
      421 ttcaagcgcc acggcggcgt tgaggtaatc gggttgatct tgcggcccca gcggtggggt
      481 gcggtaaaac gaagaaacgg caagaatgcg gctttcaggg atatcgccta atgctttcag
      541 ggcagcattg acctgctcca gcggagaggc cagattgctg cctatggcaa tatacgccac
      601 tgtcatgtgg taccctcacg acgcggtgcg cgtttgcgtg gacgacgagt acgacgacgc
      661 ggtgacggtt cttcatccag ctcgttaagc atcccttttt gatctggtgg tgcggaaacc
      721 tggaactcac cccaccattt caccagacgc tgcagttcag cattacgttc aacttcagct
      781 cgcaaggcca acaggtcata agccgcacgg aacttaggat gctccagcag tttccatgcg
      841 cgtttaccct gacgacggga catacgcaac tgcaactgcc agatatcgcg ggttaacgtc
      901 gtcagacgtt tcgggattgc cagtgaacgg caggcttcgt ccagcacgtc gttcatcgcc
      961 agcgcgaaag cgtcgtgata ggtcaggccg ctttcctggg cgatcttctg tgccgtctcc
     1021 agcagtgggt accagaacat ggcggcaaac aagaacgccg ggttcacgcg catatcgtta
     1081 tggatacgcg tatcggtatt cttcagcacc tgttcaatga tccgctccat cgggctgtcg
     1141 ccattttccg tgaagtagcg ggtaatggtc gggaacagcg gctggaacag atgatattca
     1201 cacaacagct tataggtttc gtaaccgtag cccgcttgta gcagtttaag cgattcttca
     1261 aacaggcgtg ccggtgggat atcgttcagc agggtagcga ggcgagggat cggttctgcg
     1321 gtttccgggc tgatgcgcat acccaatttg gcggcaaaac gtaccgcgcg cagcatacgt
     1381 accggatctt cacggtagcg cgtttccggg ttaccaatca gacggataac gccgtccttc
     1441 agatccttca taccgccaac gtaatcacgg acggtaaaat ccgctacgct gtaatacagg
     1501 ctgttgatag tgaaatcgcg gcgctgggcg tcttcttcga tggagccgaa aatgttgtcg
     1561 cgcagcaaca tgccgttttg cccgcgttgg gaggtcgtgc ggtcgctgac gttaccttcg
     1621 tggtgtccac ggaaggtcgc aacttcgata atctccgggc caaacattac atgagccaga
     1681 cggaaacggc gacccaccag gcggcagtta cggaacagtt tgcgcacctg ctcaggcgtg
     1741 gcgttagtgg ttacgtcaaa atctttcggc tttttgccaa gtaacaggtc gcgcacgccg
     1801 ccgccaacca gccaggcttc gtatcccgct ttgttgagcc tgtacattac cttcagggca
     1861 ttttcactga tatctttgcg ggaaatagca tgctgctcac gcgggatcac tgtcacctgt
     1921 ggacgggcga ctgcctgttc agcctcgctt tcctcgcggc ttagcacctt gcggcaaaaa
     1981 ttagcgactc gggtaaaaat agtacacctc ggtagtgtca aacatcattc aggacaaaaa
     2041 aatagcggct aatcatagct cagcatgacg catttgagaa tgttgaattt acaattgccg
     2101 actcgggcac ggcggtcagc atccagtttt tgacggctga ctgaaggatt tgctcgacgc
     2161 tgaaatcctg ccagtgtgct tctgcctgct gccccagaaa ttgaagtgcc gcgattagta
     2221 ccgggcgtgg atcgcctttc ggcaacgcag gcgcatgatt ctgcttggaa agtttagcgc
     2281 cttgtggatt aagcgccagc ggcagatgaa tgtaatctgg cactttccag ccaaaaagct
     2341 ggtacagcga gatttgcctt actgttggtt caatcagatc agccccacgc actatttctg
     2401 taacgccctg gaaatgatca tcaaccacaa cagccaggtt gtaggcgaac aacccatcac
     2461 ggcgatgaat gataaaatct tcccgtgcca gtttttcgtc ggcgtgaata atgcctcgca
     2521 gcaggtcagt aaattgcgtg accggatgct gctggcggat acgcactgcg gcgttgtctg
     2581 gtccatgatg caacacccgg caatgaccgt cgtaaatacc gccaatgctt tgaatacgcg
     2641 cacgcgtaca ggtgcagtaa taacttagtc cttgttcatg taaccaggcg agtgcttcac
     2701 gataggcgtc gtgacgttgc gattgccaga gaacatcgcc gtcccagtgc agaccgtaat
     2761 gttccagctg gcgcaggata gtttctgcgg caccgggaac ttcacgaggc gggtcgatat
     2821 cttctatgcg taccagccag cgaccttgcc gggcgcgacc ctgcaaatag ctgccgagcg
     2881 cggtgatcag agagccaaaa tgaagctcgc cggaaggaga gggggcgaag cggccaatat
     2941 actgtgtgtc tgtcatctct ttgaacaaaa aataaggcgg gagcatttcc cgcctgtggt
     3001 aaacgtgatg gaacggctgt aattagccag ccatctgttt ttcgcgaatt tcagccagcg
     3061 ttttgcagtc gatgcacaga tcggctgtcg ggcgcgcttc cagacggcga ataccaattt
     3121 caacaccgca ggattcgcag tagccgaaat cttcgtcttc cacttttttc agcgtcttct
     3181 cgatcttttt gatcagctta cgctcgcgat cgcggttacg cagttcgagg ctgaactctt
     3241 cttcctgggc tgcacggtct accgggtccg ggaagttggc tgcttcatcc tgcatatgtg
     3301 taacggtgcg atcgacttca tccctgagtt gattacgcca tgcttccaga atacgacgga
     3361 agtgcgccag ctgggcttca ttcatatact cttcgcccgg cttctcctga tatggttcca
     3421 ccccagcgat ggcgagaata ctcagggacg atgttttacg gttttgccct tcttgcatgt
     3481 tgcttctcct taacacgcac tatcgatccc catgttcggg ggaaaaatga ggccgctata
     3541 aatagcagat gcttttccgg atagcaatta tctaaacgta acacttgaca actgtgtgag
     3601 gaaaagcgta ttcgcgcacg cgaccagaat gtaaattaac cagttactta ctttactaca
     3661 atgtaaccgg cagtgatttt ttaagagcca tgccttcagc agaaatttcc gctttgtaag
     3721 ccagaatttc tacccccctc tgttgagctt ctgacaatag ttgcgcgtat ttctcatcga
     3781 tgtggcgcgc gggtgaaaac cgtgtaatgg ctgaatgcag cacggcgaaa aagataaccg
     3841 cacgctggcc ttcagccgct acgctcatca actcccgaag gtgtttctga cctcgttcag
     3901 tgaccgcatc gggaaaatat ccctgttcgt tctccgctaa cgtaaccgat ttcacttcaa
     3961 tatagcagtc tggacgcgaa tccgcctgca acataaagtc aatacggctg cgttcggagc
     4021 cgtattttac ttcgcttttc agcgagctat agcctgacag ttctgaaatt gattcattaa
     4081 ggatagcctc tttcgtcaac ctgttagccc aaagcgtatt gacgcaaata aatgcgccgc
     4141 tctggctttg agttaattcc caggtgtgtg ggtatttccg tttggtgttg tctgaagtcg
     4201 aataccagac ggtatcgcca ggcgttgcac aaccggtcat cgcacccgta ttcgggcagt
     4261 gtagcgttaa ttcgcgacca tcgggtgtga tcacatcggc taaaaaacgt ttgtaacgct
     4321 gaattagcgt cgcgcgctgt agagggggag aaaattccat tgcgacaatc cttgttattg
     4381 cgttagcgcc cagcgtttta gcggcgtgta gcgtgtgcgt ccacgggcaa acgaggaggc
     4441 gtaaagggtg aactccgtca ccgcataaga ccagttaaaa cccggcggcg ggaatgtcac
     4501 cgcctcgctg gcgtcgcgca ataaggtaat atgtggatga aacggacgat tgctttgaaa
     4561 acaaccgctg cgggcagcct gtgaacggag catattcgcc agctggatta agccgcgtgg
     4621 aggctgacgc atccctaacc acaccacacg cgaacgcagc cattgtccgg cgtcatcaag
     4681 cgtgagtgtg aaacctggtt gacgaatccg tccggctaaa agagaaagcg ccttctcttt
     4741 ctctgcgctc acttcgccta aaaatgccag agtcagatgc aaattatcgg cggcgaccgg
     4801 acgtcccgcc tcaggtggga agtgtgtggc gcgccaatgg ataatctgtt cgcgggtttc
     4861 tgcaggtaag tcgatagcaa agaacagacg ttgcggttca gacatgtgag gcactcggtt
     4921 atgaattacc gcgatgctac aatgtggcgc gaagaatgtt aaccctctgg agcgttttgt
     4981 gtcgtcgttg cccgttgctg ccgtcttacc tgaattactt accgctctcg attgtgcgcc
     5041 gcaggtatta ttaagtgcgc cgaccggggc cgggaaatca acctggctgc cgctgcaact
     5101 gctggcgcat cccggcatta acgggaaaat tatcctgctg gagccgcgtc gtctggcggc
     5161 gcgtaacgtc gcgcaacggc tggcggagct gcttaacgaa aagccaggcg ataccgttgg
     5221 ctaccggatg cgtgcgcaaa actgcgtcgg gccgaatacc cgcctggaag tggttaccga
     5281 aggcgtgctg acgcggatga tccagcgtga cccggaactg agcggtgttg gattggtgat
     5341 ccttgatgag tttcatgagc gcagtttgca ggcggatttg gcgttggcgc tgttactcga
     5401 tgtgcaacaa ggtctgcgtg atgaccttaa actgctgatt atgtcggcta cgctggacaa
     5461 tgaccgcttg cagcaaatgc tgccggaagc gcccgtcgtc atctcagaag ggcgctcgtt
     5521 tccggttgaa cgccgttatt taccgctgcc cgcgcatcag cgttttgacg aagccgttgc
     5581 ggtagccacc gccgaaatgg tgcgtcagga aagtggatca ttactgttat ttttacctgg
     5641 cgtcggagaa attcagcgcg tgcaggaaca actggcttcg cgcatcggca gtgatgtggt
     5701 gctctgcccg ctgtatggcg cgttgtcact gaacgatcag cgaaaagcga tcctcccggc
     5761 accgcaaggg atgcgcaaag tggtgctggc gaccaatatt gctgaaacca gtttaaccat
     5821 cgaaggtatt cgtttggtgg tggattgtgc ccaggagcgt gtggcgcgct ttgatccgcg
     5881 cacggggctt acgcggttga ttactcaacg cgttagtcag gcatccatga cgcagcgtgc
     5941 cgggcgcgcc gggcgtctgg agccgggtat cagcctgcat ttaatcgcca aagaacaagc
     6001 agaacgcgcc gcggcgcaaa gtgagccgga gatcttacaa agcgatcttt ccggtttgct
     6061 gatggaatta ctgcaatggg gatgcagcga tccggcgcag atgagctggc tggatcaacc
     6121 gccaacggtg aatctactgg ccgcgaaacg cctgttacaa atgctggggg cgctggaggg
     6181 tgaacggctt agtgcgcaag ggcaaaaaat ggcagcgctg ggtaacgatc cgcgtttagc
     6241 ggcaatgctg gttaacgcaa agagcgatga cgaagctgct accgcggcaa aaattgccgc
     6301 cattctcgaa gagccgccac ggatgggcaa tagtgacctg ggcgtggcgt tttcgcgcaa
     6361 tcaaccagcc tggcagcaac gtagtcagca actgttaaaa cgcttaaacg tacgcggcgg
     6421 tgaggcagac agttcgctta tcgcgccgct acttgccggg gcgtttgccg atcgcattgc
     6481 tcatcgccgt gggcaagatg gacgctatca actggcaaac ggcatgggag cgatgctcga
     6541 tgccgacgac gcgctaagcc gccacgaatg gttgatcgca cagttattat tgcagggcag
     6601 cgcctcgccg gatgcgcgga ttttactggc gctaccggtc gatattgatg aattagtaca
     6661 acgctgtccg cagctggtgc agcaatctga caccgtggaa tgggatgacg cgcaaggtac
     6721 gctgaaagcc tggcgtcggc tacaaatcgg tcagttgacg gtgaaagtgc agccgctggc
     6781 gaaaccgtca gaagacgagt tgcatcaggc gatgcttaat ggcatccgtg ataaaggttt
     6841 aagcgtgctc aactggacgg cggaagcgga acagctacgc ttgcgtttgt tatgcgccgc
     6901 aaagtggttg ccggaatatg actggccagc ggttgatgat gaaagtttgt tggcgacgct
     6961 ggaaacgtgg ctgctgccac atatgaccgg cgtacattca ctacgcggcc tgaaatcact
     7021 cgacatttat caggcactac gcggattact tgattgggga atgcagcaac gtctggatag
     7081 tgaattgcct gcgcattaca ctgtgccgac gggaagccgg atcgccattc gttatcatga
     7141 agataacccg cccgcgctgg cggtgagaat gcaggagatg tttggtgagg ccactaatcc
     7201 gacgatcgcc caggggcgcg tgccgctggt gctgaagttg ctttcccctg cccaaaggcc
     7261 gctgcaaatc acgcgtgatt tgggggcctt ctggaaaggg gcgtatcgtg aggtgcaaaa
     7321 ggagatgaaa gggcgttatc ccaaacacgt ctggccgaac gacccggcaa acacggcacc
     7381 gacgcgacgg acgaaaaagt attcgtaagg ttggttttct ccctctccct gtgggagagg
     7441 gccggggtga gggcatcagc gcgcacgttc accctcaccc taaccctctc cctcaaggga
     7501 gaggggaccg atcgagcaca aattttgaga gatatcttct tctgtcttgt aacagaagaa
     7561 cagaaaatcg ggcttttgcg cctgaatatt gcggagaaaa agcatggccg ggaatgaccg
     7621 cgagccaatt ggacgcaaag ggaaaccgac gcgtccggtc aaacaaaagg taagccgtcg
     7681 tcgttacgaa gatgacgatg attacgacga ttatgatgac tatgaggatg aagaaccgat
     7741 gccgcgcaaa ggtaagggca aaggcaaagg gcgtaagcct cgtggcaaac gcggctggct
     7801 atggctactg ctaaaactgg ctatcgtttt tgccgtgctg atcgccattt acggcgttta
     7861 tctcgatcaa aaaattcgta gccgtattga tggcaaggtc tggcaactgc ccgcggcagt
     7921 ttatggccga atggtcaacc ttgaaccaga tatgaccatc agcaagaacg aaatggtgaa
     7981 gctgctggag gcaacacagt atcgtcaggt gtcgaaaatg acccgtcctg gcgaatttac
     8041 cgtgcaggcc aacagcattg agatgatccg ccgtccgttt gatttcccgg acagtaaaga
     8101 agggcaggtg cgtgcgcgtc tgacctttga tggtgatcat ctggcgacca tcgtcaatat
     8161 ggagaacaac cgtcagttcg gtttcttccg tcttgatccg cgtctgatca ccatgatctc
     8221 ctcgccaaac ggtgagcagc gtttgtttgt gccgcgcagt ggtttccctg atttgctggt
     8281 ggatactttg ctggcgacag aagaccgtca tttttacgag catgatggaa tcagtctcta
     8341 ctccattggg cgtgcggtgc tggcaaacct gaccgcaggt cgcacggtgc agggtgcgag
     8401 tacgctgacg caacagctgg tgaaaaacct gttcctctcc agcgagcgtt cttactggcg
     8461 taaagcgaac gaagcttaca tggcgctgat catggacgcg cgttacagca aagaccgtat
     8521 tcttgagctg tatatgaacg aggtgtatct cggtcagagc ggcgacaacg aaatccgcgg
     8581 cttcccgctg gcaagcttgt attactttgg tcgcccggta gaagagctaa gcctcgacca
     8641 gcaggcgctg ttagtcggta tggtgaaagg ggcgtccatc tacaacccgt ggcgtaaccc
     8701 gaaactggcg ctggagcgac gtaatctggt gctgcgtctg ctgcaacagc aacagattat
     8761 tgatcaagaa ctctatgaca tgttgagtgc ccgtccgctg ggggttcagc cgcgtggtgg
     8821 ggtgatctct cctcagccag cctttatgca gttggtgcgt caggagttgc aggcaaaact
     8881 gggcgataag gtaaaagatc tctccggcgt gaagatcttc actacctttg actcggtggc
     8941 ccaggacgcg gcagaaaaag ccgccgtgga aggcattccg gcactgaaga aacagcgtaa
     9001 gttgagcgat cttgaaactg cgattgtggt cgtcgaccgc tttagtggtg aagttcgtgc
     9061 gatggtcgga ggttctgagc cgcagtttgc gggctacaac cgtgcgatgc aggcgcgtcg
     9121 ttcgattggt tcccttgcaa aaccagcgac ttatctgacg gctttaagcc agccgaaaat
     9181 ctatcgcctg aacacgtgga ttgccgatgc gccaatcgcg ctgcgtcagc cgaatggcca
     9241 ggtctggtca ccgcagaacg atgaccgtcg ttatagcgaa agcggcagag tgatgctggt
     9301 ggatgcgttg acacgttcga tgaacgtgcc gacggtaaat ctggggatgg cgctggggct
     9361 gcctgcggtt acggagacct ggatcaaact gggcgtaccg aaagatcagt tgcatccggt
     9421 tccggcaatg ctgctggggg cgttgaactt aacgccaatc gaagtggcgc aggcattcca
     9481 gaccatcgcc agcggtggta accgtgcacc gctttctgca ctgcgttcgg taatcgcgga
     9541 agatggtaag gtgctgtatc agagcttccc gcaggcagaa cgcgctgttc cggcgcaggc
     9601 ggcgtatctg acactatgga ccatgcagca ggtggtacaa cgtggtacgg gtcgtcagct
     9661 tggggcgaaa tacccgaacc tgcatctggc agggaaaaca gggactacca acaataacgt
     9721 agatacctgg tttgcaggca ttgacggcag cacggtgacc atcacctggg taggccgtga
     9781 taacaaccag ccgaccaaac tgtatggagc cagcggggca atgtcgattt atcagcgtta
     9841 tctggctaac cagacgccaa cgccgctgaa tcttgttccg ccagaagata ttgcagatat
     9901 gggcgtggac tacgacggca actttgtttg cagcggtggc atgcgtgtct tgccggtctg
     9961 gaccagcgat ccgcaatcgc tgtgccagca gagcgagatg cagcagcagc cgtcaggcaa
    10021 tccgtttgat cagtcttctc agccgcagca acagccgcaa cagcaacctg ctcagcaaga
    10081 gcagaaagac agcgacggtg tagccggttg gatcaaggat atgtttggta gtaattaaca
    10141 tctaagcgtg aaataccgga tggcgagttg ccatccggta aaataacatc ccatctaaga
    10201 tattaaccct ttcttttcat ctggttgttt attaaccctt caggaacgct cagattgcgt
    10261 accgcttgcg aacccgccag cgtttcgaat attatcttat ctttataata atcattctcg
    10321 tttacgttat cattcacttt acatcagaga tataccaatg gcgcgttcca aaactgctca
    10381 gccaaaacac tcactgcgta aaatcgcagt tgtagtagcc acagcggtta gcggcatgtc
    10441 tgtttatgca caggcagcgg ttgaaccgaa agaagacact atcaccgtta ccgctgcacc
    10501 tgcgccgcaa gaaagcgcat gggggcctgc tgcaactatt gcggcgcggc agtcagctac
    10561 cggcactaaa accgatacgc cgattcaaaa agtgccacag tctatttctg ttgtgaccgc
    10621 cgaagagatg gcgctgcatc agccgaagtc ggtaaaagaa gcgcttagct acacgccggg
    10681 tgtctctgtt ggtacgcgtg gcgcatccaa cacctatgac catctgatca ttcgcggttt
    10741 tgcggcagaa ggccaaagcc aaaataacta tctgaatggc ctgaagttgc agggcaactt
    10801 ctataacgat gcggtcattg acccgtatat gctggaacgc gctgaaatta tgcgtggccc
    10861 ggtttccgtg ctttacggta aaagcagtcc tggcggtctg ttgaatatgg tcagcaagcg
    10921 tccgaccacc gaaccgctga aagaagttca gtttaaagcc ggtactgaca gcctgttcca
    10981 gactggtttt gactttagcg atgcgttgga tgatgacggt gtttactctt atcgcctgac
    11041 cggtcttgcg cgttctgcca atgcccagca gaaagggtca gaagagcagc gttatgctat
    11101 tgcaccggcg ttcacctggc gtccggatga taaaaccaat ttcaccttcc tttcttactt
    11161 ccagaacgag ccggaaaccg gttattacgg ctggttgccg aaagagggaa ccgttgagcc
    11221 gctgccgaac ggtaagcgtc tgccgacaga ctttaatgaa ggggcgaaga acaacaccta
    11281 ttctcgtaat gagaagatgg tcggctacag cttcgatcac gaatttaacg acacctttac
    11341 tgtgcgtcag aacctgcgct ttgctgaaaa caaaacctcg caaaacagcg tttatggtta
    11401 cggcgtctgc tccgatccgg cgaatgctta cagcaaacag tgtgcggcat tagcgccagc
    11461 ggataaaggc cattatctgg cacgtaaata cgtcgttgat gatgagaagc tgcaaaactt
    11521 ctccgttgat acccagttgc agagcaagtt tgccactggc gatatcgacc acaccctgct
    11581 gaccggtgtc gactttatgc gtatgcgtaa tgacatcaac gcctggtttg gttacgacga
    11641 ctctgtgcca ctgctcaatc tgtacaatcc ggtgaatccc gatttcgact tcaatgccaa
    11701 agatccggca aactccggcc cttaccgcat tctgaataaa cagaaacaaa cgggcgttta
    11761 tgttcaggat caggcgcagt gggataaagt gctggtcacc ctaggcggtc gttatgactg
    11821 ggcagatcaa gaatctctta accgcgttgc tgggacgacc gataaacgtg atgacaaaca
    11881 gtttacctgg cgtggtggtg ttaactacct gtttgataat ggtgtaacac cttacttcag
    11941 ctatagcgaa tcgtttgaac cttcttcgca agttgggaag gatggtaata ttttcgcacc
    12001 gtctaaaggt aagcagtatg aagtcggcgt gaaatatgta ccggaagatc gtccgattgt
    12061 agttactggt gccgtgtata atctcactaa aaccaacaac ctgatggcgg accctgaggg
    12121 ttccttcttc tcggttgaag gtggcgagat ccgcgcacgt ggcgtagaaa tcgaagcgaa
    12181 agcggcgctg tcggcgagtg ttaacgtagt cggttcttat acttacaccg atgcggaata
    12241 caccaccgat actacctata aaggcaatac gcctgcacag gtgccaaaac acatggcttc
    12301 gctgtgggct gactatacct tctttgacgg tccgctttca ggtttgacgc tgggcaccgg
    12361 tggtcgttat actggctcca gctatggtga tccggctaac tcctttaaag tgggaagtta
    12421 tacggtcgtg gatgcgttag tgcgttatga tctggcgcga gtcggcatgg cgggctccaa
    12481 cgtggcgctg catgttaaca acctgttcga tcgtgaatac gtcgccagct gctttaacac
    12541 ttatggctgc ttctggggcg cagaacgtca ggtcgttgca accgcaacct ttcgtttcta
    12601 atttctcttt tggggcacgg atttccgtgc ccatttcaca agttggctgt tatgcaggaa
    12661 tacacgaatc attccgatac cacttttgca ctgcgtaata tctcctttcg tgtgcccggg
    12721 cgcacgcttt tgcatccgct gtcgttaacc tttcctgccg ggaaagtgac cggtctgatt
    12781 ggtcacaacg gttctggtaa atccactctg ctcaaaatgc ttggtcgtca tcagcctccg
    12841 tcagaagggg agattcttct tgatgcccag ccgctggaaa gctggagcag caaagcgttt
    12901 gcccgcaaag tggcttattt gccgcagcag cttcctccgg cagaagggat gaccgtgcgt
    12961 gaactggtgg cgattggtcg ttacccgtgg catggcgcgc tggggcgctt tggtgcggca
    13021 gatcgcgaaa aggtcgagga agctatctcg ctggttggct taaaaccgct ggcgcatcgg
    13081 ctggtcgata gtctctctgg cggcgaacgt cagcgggcgt ggatcgccat gctggtggcg
    13141 caggatagcc gttgtctgtt gctcgacgaa ccgacttcgg cgctggatat cgcccaccag
    13201 gttgatgtgc tggcgctggt gcaccgttta agtcaggaac gtggcctgac ggtcattgcc
    13261 gtgttgcacg atatcaatat ggcggcacgc tactgtgatt atctggtcgc cctgcgcagt
    13321 ggtgaaatga ttgctcaggg aacgcctgcg gaaattatgc gcggcgaaac cctcgaaatg
    13381 atttatggca tcccgatggg tattttgccg catccggcgg gtgctgcacc tgtgagtttt
    13441 gtttattgat gagcggctta cctcttattt cgcgccgtcg actgttaacg gcgatggcgc
    13501 tttctccgtt gttatggcag atgaataccg cccacgcggc ggctattgac cccaatcgta
    13561 ttgtggcgct ggagtggttg ccggtggaat tactgctggc gctcggcatc gtgccttacg
    13621 gcgtggcgga taccatcaac tatcgcctgt gggtcagcga accaccattg ccggactcag
    13681 tgattgacgt cggtttgcgt acagaaccta accttgaact gctgaccgaa atgaaaccgt
    13741 cgtttatggt ctggtcggca ggatatggcc cttcatcaga aatgctggca cgtattgcgc
    13801 cgggtcgcgg atttaacttc agtgacggca aacatccgtt ggcgatggcg cgtaaatcgc
    13861 tgacggaaat ggcagattta cttaacctgc aaagcgcagc ggaaacgcat ttagcgcaat
    13921 acgaagactt tatccgcagc atgaaacccc gctttgtgaa gcgtggtgca cgcccgttac
    13981 tgctgacgac gcttatcgat ccgcgccata tgctggtctt cggtccaaac agcttgttcc
    14041 aggaaattct cgatgagtac ggcatcccaa atgcctggca aggggaaacc aacttctggg
    14101 gcagtaccgc cgtcagtatc gatcgtctgg cagcgtataa agacgttgat gtgctctgtt
    14161 ttgatcacga caacagcaaa gacatggatg cgctaatggc aaccccgctg tggcaggcca
    14221 tgccgtttgt ccgtacaggg cgctttcagc gcgtacccgc agtctggttt tatggtgcaa
    14281 cgctttcggc aatgcacttt gtgcgtgttc tggataacgc catcggaggt aaagcgtgag
    14341 taaacgaatt gcgcttttcc cggcgttatt gctggcgctg ttagtgattg tcgctacggc
    14401 gctcacctgg atgaacttct cacaggcgct gccgcgcagt cagtgggcgc aggccgcctg
    14461 gtcgccggat attgacgtca tcgagcagat gatttttcac tacagcttgt tgccgcgtct
    14521 ggcgatttcg ctgctggtgg gcgcgggcct ggggctggtg ggcgtgctgt ttcagcaagt
    14581 actgcgtaac ccgctggcgg aaccgacgac gttgggcgtt gcaacaggcg cgcaactggg
    14641 gattaccgtc accacgctct gggcgatccc tagcgcgatg gcgagccagt ttgctgcgct
    14701 ggcaggggct tgcgttgttg gcttaattgt ctttggcgtc gcgtggggga aacggctgtc
    14761 gccggtaacg ctgattctcg cggggttggt agtgagcctt tattgcggcg caatcaatca
    14821 gttactggtt atcttccatc atgaccagtt gcaaagcatg tttctgtgga gcactggaac
    14881 gctgacgcaa accgactggg gcggtgttga gcgtttatgg ccgcagttgc tgggcggtgt
    14941 gatgctgacg ttgctgctac ttcgtccgtt aaccctgatg gggcttgatg atggcgtggc
    15001 gcgcaatctc gggctggcct tgtcgcttgc gcgtctggcg gcgctgtcgc tggcgattgt
    15061 catcagtgcg ctgctggtga acgctgtggg gattatcggc tttatcgggt tgttcgcgcc
    15121 gctgctggca aaaatgctgg gggcgcggcg tctgctgcca cgactgatgc tggcgtcgtt
    15181 gattggtgcg ctgatcctct ggctttccga tcaaatcatc ctctggctga ctcgcgtgtg
    15241 gatggaagtg tccaccggtt cggtcactgc gttgatcggt gcgccgctgc tactgtggct
    15301 gctgccgcgt ttacgcagca ttagcgcgcc ggatatgaag gttaacgatc gtgtcgcgac
    15361 tgaacgccaa catgtgctgg cgtttgccct cgcgggcggc gtgctgttgt tgatggctgt
    15421 ggtggtggcg ctgtcgtttg gtcgtgatgc gcacggctgg acgtgggcga gcggggcgtt
    15481 gctcgaggat ttaatgccct ggcgctggcc gcgaattatg gcggcgctgt ttgcgggcgt
    15541 catgctggcg gtagcgggct gtattattca gcgactgacc ggcaacccga tggcaagccc
    15601 ggaagtgctg gggattagct ccggcgcggc gtttggtgtg gtgttaatgc tgtttctggt
    15661 accgggtaat gcctttggct ggttgttacc cgcaggcagt ctcggggcgg cggtgatgct
    15721 gttgatcatt atgatcgccg ccggacgcgg tggattttcc ccacaccgca tgttactggc
    15781 ggggatggcg ttaagtaccg cgttcaccat gcttttgata atgttgcagg caagtggtga
    15841 cccgcgaatg gcgcaagtgc tgacctggat ttccggttcg acctacaacg cgaccgatgc
    15901 tcaggtctgg cgcaccggaa ttgtgatggt gattttgctg gcgattactc cgctgtgccg
    15961 ccgctggctg accattttac cgctgggtgg tgataccgcg cgggcggtag ggatggcgct
    16021 gacgccgacg cgaattgcgc tgctgctgtt agcggcttgc ctgacggcga ccgcgacgat
    16081 gactattgga ccgttgagtt ttgttggttt aatggcaccg catattgcgc ggatgatggg
    16141 ctttcgacgg acgatgccac atatcgtaat ttcggcgctg gtgggtggtt tactgctggt
    16201 gttcgctgac tggtgtgggc ggatggtgct gtttccattc cagatcccgg cggggctgct
    16261 gtcgaccttt atcggtgcgc catattttat ctatttgctg agaaagcaga gccgttaata
    16321 gaatgcctcg cccggtgggc gaggcaaaat tatcacaact tcgcgaacac ccgacgtgca
    16381 gcatcgatgg tgttattgat atcttccatg ctgtgcgcca cagacataaa gcccgcttca
    16441 aacgctgacg gtgccaggta aacaccttca tccagcatca tatggaagaa acgcttaaaa
    16501 cgctcgacgt cacaggccat cacatcctga tagcacgtca cggactcggc gtcggtaaag
    16561 aagataccga acataccacc gacgtggtta acgaccagcg gaattcccgc ttcttctgcc
    16621 gcttccagca gaccttctgc cagacgtgtt gtcagctcat ccagcgtttc gtgaatgccc
    16681 ggctgcgcga cttcattcag acaggcgaaa cccgctgcca tcgcaatcgg gttaccggaa
    16741 agcgtacccg cctgatagac cggacctgtc ggggccagcg catccattac atcacgacga
    16801 ccaccgaatg cgcctaccgg cattccaccg ccgatgattt ttccgaggca ggttaaatct
    16861 ggcactacgc cgaaataatc ctgtgcgcca gctagcgcta cgcggaaacc ggtcatcact
    16921 tcatcgatga tcagcaacgc gccaaattcg tcgcacagcg cacgcagacc tggcaggaac
    16981 tctggcagcg gcggaacaca gttcatattg cctgccaccg gctcgacgat aatacaggca
    17041 atctcttgcg ggtattgctc aaacgcggcg cgtacagagg ccagatcgtt ataagtacag
    17101 gttaaggtat gtttggcgaa atctgccgga acgcccggcg agtttggctg gcctaacgtg
    17161 agtgcgccag aaccggcttt caccagcagg cagtcagcgt gaccgtggta acaaccttca
    17221 aatttaatga ttttgtcgcg accggtaaaa ccacgggcca ggcggatggc gctcatcgtc
    17281 gcctcggtgc cggagttcac catgcgcacc atatccatgg tcgggaccag ttcggtcacc
    17341 agttgcgcca ttttcacttc catttcggtc ggtgcaccaa agcttaaacc acgctcggcg
    17401 gcttcaatca cggcattgcg gatcgccgga tggttatggc ccagcaccat cggcccccag
    17461 gaaccgacat aatcgatata ggctttgcca tcaacatcgt acagataagc gccgtccgct
    17521 ttttcgataa acagtggagt gccgcccacg ccagtaaagg cgcgaacagg ggagttcaca
    17581 ccgccaggga tcagctcgcg cgctgcgctg taaagatttt cagacttact catggagggg
    17641 tcctgattcg tagaaaaagt gaatggctgc tattctatgt tattcataac aagttaaata
    17701 ctcgtcaaac atcaggctgc ttgtactggt gcaatcctga tttcttagag tataaaagtt
    17761 ttgtgcattt gaaacattac gctttgcaaa ggattttcat ggaacgtgcg agtaaaatgc
    17821 cgtcatctta tttgtatgac caataagtga tcattggatg aaaactgata ctccctcttt
    17881 agaaacaccg caggccgcgc gcctgcgacg cagacaactg attcgccaac ttcttgagcg
    17941 cgataaaacc ccgttagcca ttttgtttat ggcggcagtc gtcggcacgc ttatcgggct
    18001 ggctgcggtt gcttttgaca aaggtgtcgc ctggttgcag aaccaacgta tgggggcgct
    18061 ggtacatact gctgataatt atccgctact gttaaccgtc gcttttctct gttcggcggt
    18121 gctggcgatg ttcggctact ttttggtgcg caaatacgcg cccgaagcag ggggatcggg
    18181 gatcccggaa attgaagggg cgctggaaga tcaacgtcct gttcgctggt ggcgtgtatt
    18241 gccggtgaaa ttctttggcg ggctggggac actcggcgga ggcatggtgc tagggcgcga
    18301 aggaccaacc gtgcagatcg gcggcaatat cggtcgcatg gtgcttgata ttttccgcct
    18361 gaaaggtgat gaagctcgcc atacgctgct ggcaaccggt gctgctgcgg ggctggctgc
    18421 ggcctttaac gcgccgctgg cgggtatttt gtttattatc gaagagatgc gtccgcagtt
    18481 tcgctatacg ttaatttcga ttaaagcggt atttattggc gtcattatgt cgaccattat
    18541 gtaccggatt tttaatcatg aagttgcgtt gattgacgtc ggtaaacttt ctgatgcgcc
    18601 gcttaatacg ctgtggcttt atctgatcct cggtattatt tttggcattt tcggccctat
    18661 ttttaataaa tgggtgctgg ggatgcagga tttgctgcac cgtgtgcacg gcggcaatat
    18721 taccaaatgg gtactgatgg ggggggcgat tggcggtctg tgtggattgc tggggtttgt
    18781 ggcaccagca acgtcgggcg gcggttttaa cctgattcct atcgctaccg cggggaattt
    18841 cagcatggga atgctggtgt ttatcttcgt cgctcgggtc attaccacct tactctgctt
    18901 ctcttccggc gcgccgggcg gtatttttgc cccgatgctg gcgctgggta ctgtgctggg
    18961 aaccgctttc ggaatggttg ccgttgagct gtttccgcaa tatcacctgg aggcggggac
    19021 gtttgctatt gccggaatgg gggcattact ggcggcatct attcgcgcgc cgttaacggg
    19081 gatcattctg gttctggaga tgaccgataa ctaccagctc attttgccaa tgattattac
    19141 cggtcttggc gcaacactat tagcgcaatt taccggcggg aaacctctat actcggcgat
    19201 tcttgcgcgc acgctggcaa aacaggaagc tgagcaactg gcgcgaagca aggccgcatc
    19261 agccagcgag aatacttgaa cgaaatacca gggtattaga taatggcgat tattattggg
    19321 ttagaatttg cccgattgcc gatgtcgttt ggagcaaaat atgagtgatg acgtagcact
    19381 gccgctggag tttaccgacg cagcagccaa caaagttaaa agcctgatcg ctgacgaaga
    19441 taacccgaat ctgaaattac gcgtgtatat caccggtggc ggttgcagcg gcttccagta
    19501 tggtttcacc tttgatgatc aggtgaacga aggcgatatg accatcgaaa aacagggcgt
    19561 tggcctggtg gttgatccga tgagcctgca atatctggtc ggcggttccg ttgactacac
    19621 cgaaggactg gaaggttctc gtttcatcgt gaccaacccg aacgcgaaaa gcacctgcgg
    19681 ttgcggttct tcctttagca tctaatctgt tgttcatgtg ccggataaac tatccggcac
    19741 atcttctctc ttcaacgccc attctcatcc agcgcaaacg tcggtagctt aagatgccaa
    19801 cgaatagccg ccagccgaat caatagcgtc acgaccatgc ccatcatact ggctgtttcc
    19861 agtggtacgg aaaatgtgta ataagccgta gcgtggacaa taccgccgat aatacaggca
    19921 gttgcgtaga tttctgtacg taaaatcatg gagatttcgc gggccagtac atcacgaatg
    19981 atcccgccgc caacgccagt aatgacgccc atacaaaccg cgattaacgg accggcttcc
    20041 gcattaaagg ctttattcac gccaatgccg acaaacaccg ccagaccaac ggcgtccagc
    20101 accggcaaca tccattttgg taagcgtctt ggctggcgca ccagcacgat ggtcagcatg
    20161 ctggtgacca tggcaacgac cagatcggtg ggatctttca cccaaaatac cgggccgtga
    20221 tccagcgcca tgtcgcgaat tgttccgtcg cctactgcgg taaccacgcc cagtaccaga
    20281 acaccaaaag ggtccatacg caattttccg gctaacaaaa cgccggagat ggcaaatacc
    20341 gctgtgccga ctatatccag ccaatagacg agcattgttc aatccccact gagcacctga
    20401 aaaggtcagg cactaatcta cctgtgaaag cgcattacag agctgttgtg ctgcgaggat
    20461 aatacgtggg cttgcacgtt caaaccagtc actcgtgaga ggaataacgg gaattttgag
    20521 ctgttcaccc cagtattgtt tgattttagg aatttggtcc ggtccgcctg taatgacaat
    20581 cgcctgtggc gagcgtgcta acacctgttc gcggctaact tgcggccagg gaacccggct
    20641 gtctttaaag atgttttctc cgccacaaac ttcgagtacc tggttctgaa tcgactcttt
    20701 tccactggta aatggcggat taatgccgaa ttgcagaaaa acacgttttt taggtttatc
    20761 agcatattgc gctttcaatt gcacgtactg atccagcagg gattgcgcgg cttgttcggc
    20821 cttgtctggt tgcggactcc agggggccag ttgacgtaac gcattggcaa tttgttcaat
    20881 gcttgtcgca tcgacccaca tcacttttat tcccagcgaa gccagctggt caacctgccg
    20941 ctcggcatta cctccacgcc aggcaatcac cagatcgggt ttcagcgcga caatgcgttc
    21001 cagattcatc ccctgccagg tggaaacctg ctcaatcttt tgcgcttgtg gaggatagtc
    21061 ggaatagctg ctgaccccaa ccggcgtgat cccggcggca aaggcaagtt cagtgttggc
    21121 gggagaaagc gtgatgacgc gcggcgcggc gttgagccac agtggcgcga gaaaagacag
    21181 ggcgaccagc gccctgaaca gtgatttagc catgtgccag tttctgcacc agtgactcaa
    21241 ccatcaggct ggactgttta gcggcaacag ccaggaactc atcgaagcta agatgagact
    21301 gttgatcggc cacgtcggag atggcgcgta cgacaacaaa cgggacgttg aaattgtggc
    21361 agacatgggc gattgccgtc gcttccatct ctacagcaat ggcctgtggg aagttgtggc
    21421 ggattttcgc cagaccaaca gaaccgttga tgaaagcgtc gccgctaaca atcaggccac
    21481 gtacagcgtt aagattcagt tcggcaatgc aggcctcagc ggcagcgatc agtttatcgt
    21541 cagctttaaa gcctgtcgga cagcctggta actgaccgta ttcataacca aatgccgtga
    21601 catccgcgtc gtgataacgc gcttcgtccg agacaacgat atcgcccact ttcagcgttg
    21661 gtgccaggcc accggcagaa ccggtgttaa taatcacatc tggcttgcag tgttccaaca
    21721 gcagagtggc acccagcgcc gcagcgactt taccgatgcc cgatttcaga agcgcaacct
    21781 cggttccatt cagttggccg gtatagattt cgcaaccgcc gagactgata gtttgacggt
    21841 tttcgatttt gtcacgcagc agcgtaactt cttcttccat tgcaccaatg atgccgattt
    21901 tcatagattt actcgcgata agcccgattt gaaggcatag tttaccatgc gcttacgggg
    21961 aagcgtattt ctcacgcggg agaggacatg gcacagattg atttccgaaa aaaaataaac
    22021 tggcatcgtc gttaccgttc accgcagggc gttaaaaccg aacatgagat cctgcggatc
    22081 ttcgagagcg atcgcgggcg tatcatcaac tctccggcaa ttcgtcgtct gcaacaaaag
    22141 acccaggttt ttccactgga gcgcaatgcc gccgtgcgca cgcgtcttac ccactcgatg
    22201 gaagtccagc aggtgggacg ctacatcgcc aaagaaattt taagccgtct gaaagagctt
    22261 aaattgctgg aagcatacgg cctggatgaa ctgaccggcc cctttgaaag cattgttgag
    22321 atgtcatgcc tgatgcacga tatcggcaat ccgccgtttg gtcattttgg cgaagcagcg
    22381 ataaatgact ggtttcgcca acgtttgcac ccggaagatg ccgaaagcca gcctctgact
    22441 gacgatcgct gcagcgtggc ggcactacgt ttacgggacg gggaagaacc gcttaacgag
    22501 ctgcggcgta agattcgtca ggacttatgt cattttgagg ggaatgcaca aggcattcgt
    22561 ctggtgcata cattgatgcg gatgaatctc acttgggcac aggttggcgg tattttaaaa
    22621 tatacccgtc cggcgtggtg gcgtggcgaa acgcctgaga cacatcacta tttaatgaaa
    22681 aagccgggtt attatctttc tgaagaagcc tatattgccc ggttgcgtaa agaacttaat
    22741 ttggcgcttt acagtcgttt tccattaacg tggattatgg aagctgccga cgacatctcc
    22801 tattgtgtgg cagaccttga agatgcggta gagaaaagaa tatttaccgt tgagcagctt
    22861 tatcatcatt tgcacgaagc gtggggccag catgagaaag gttcgctctt ttcgctggtg
    22921 gttgaaaatg cctgggaaaa atcacgctca aatagtttaa gccgcagtac ggaagatcag
    22981 ttttttatgt atttacgggt aaacacccta aataaactgg taccctacgc ggcacaacga
    23041 tttattgata atctgcctgc gattttcgcc ggaacgttta atcatgcatt attggaagat
    23101 gccagcgaat gcagcgatct tcttaagcta tataaaaatg tcgctgtaaa acatgtattt
    23161 agccatccgg atgtcgagca gcttgaattg cagggctatc gggtcattag cggattatta
    23221 gagatttatc gtcctttatt aagcctgtcg ttatcagact ttactgaact ggtagaaaaa
    23281 gaacgggtga aacgtttccc tattgagtcg cgcttattcc acaaactctc gacgcgccat
    23341 cggctggcct atgtcgaggc tgtcagtaaa ttaccgtcag attctcctga gtttccgcta
    23401 tgggaatatt attaccgttg ccgcctgctg caggattata tcagcggtat gaccgacctc
    23461 tatgcgtggg atgaataccg acgtctgatg gccgtagaac aataaccagg cttttgtaaa
    23521 gacgaacaat aaatttttac cttttgcaga aactttagtt cggaacttcg ggctataaaa
    23581 cgaatctgaa gaacacagca attttgcgtt atctgttaat cgagactgaa atacatgaaa
    23641 aaaaccacat tagcactgag tgcactggct ctgagtttag gtttggcgtt atctccgctc
    23701 tctgcaacgg cggctgagac ttcttcagca acgacagccc agcagatgcc aagccttgca
    23761 ccgatgctcg aaaaggtgat gccttcagtg gtcagcatta acgtagaagg tagcacaacc
    23821 gttaatacgc cgcgtatgcc gcgtaatttc cagcagttct tcggtgatga ttctccgttc
    23881 tgccaggaag gttctccgtt ccagagctct ccgttctgcc aggggggcca gggcggtaat
    23941 ggcggcggcc agcaacagaa attcatggcg ctgggttccg gcgtcatcat tgatgccgat
    24001 aaaggctatg tcgtcaccaa caaccacgtt gttgataacg cgacggtgat taaagtccag
    24061 ctgagcgatg gtcgtaagtt cgacgcgaag atggttggca aagatccgcg ctctgatatc
    24121 gcgctgatcc aaatccagaa cccgaaaaaa ctgaccgcaa ttaagatggc ggattctgat
    24181 gcactgcgcg tgggtgatta caccgtagcg attggtaacc cgtttggtct gggcgagacg
    24241 gtaacttccg ggattgtctc tgcgctgggg cgtagcggcc tgaatgctga aaactacgaa
    24301 aacttcatcc agaccgatgc agcgatcaac cgtggtaact ccggtggtgc gctggttaac
    24361 ctgaacggcg aactgatcgg tatcaacacc gcgatcctcg caccggacgg cggcaacatc
    24421 ggtatcggtt ttgctatccc gagtaacatg gtgaaaaacc tgacctcgca gatggtggaa
    24481 tacgggcagg tgaaacgcgg tgagctgggt attatgggga ctgagctgaa ttccgaactg
    24541 gcgaaagcga tgaaagttga cgcccagcgc ggtgctttcg taagccaggt tctgcctaat
    24601 tcctcggctg caaaagcggg cattaaagcg ggtgatgtga tcacctcact gaacggtaag
    24661 ccgatcagca gctttgccgc actgcgtgct caggtgggta ctatgccggt gggcagcaaa
    24721 ctgaccctgg gcttactgcg cgacggtaag caggttaacg tgaacctgga actgcagcag
    24781 agcagccaga atcaggttga ttccagctcc atcttcaacg gcattgaagg cgctgagatg
    24841 agcaacaaag gtaaagatca gggcgtggta gtgaacaacg tgaagacggg cactccggct
    24901 gcgcagatcg gcctgaagaa aggtgatgtg attattggtg cgaaccagca ggcagtgaaa
    24961 aacatcgctg aactgcgtaa agttctcgac agcaaaccgt ctgtgctggc actgaacatt
    25021 cagcgcggcg acagcaccat ctacctgtta atgcagtaat ctccctcaac cccttcctga
    25081 atacgggaag gggttctcct tacaatctgt gaacttcgcc acaactccat acatcttcat
    25141 catcctttgg gcatttgcac aatgccgtac gttacgtact tccttatgct aagccgtgca
    25201 taacggagga cttatggctg gctggcatct tgataccaaa atggcgcagg atatcgtggc
    25261 acgtaccatg cgcatcatcg ataccaatat caacgtaatg gatgcccgcg ggcgaattat
    25321 cggcagcggc gatcgtgagc gtattggtga attgcacgaa ggtgcattgc tggtactttc
    25381 acagggacga gtcgtcgata tcgatgacgc ggtagcacgt catctgcacg gtgtgcggca
    25441 ggggattaat ctaccgttac ggctggaagg tgaaattgtc ggcgtaattg gcctgacagg
    25501 tgaaccagag aatctgcgta aatatggcga actggtctgc atgacggctg aaatgatgct
    25561 ggaacagtcg cggttgatgc acttgttggc gcaggatagc cgtttgcggg aagaactggt
    25621 gatgaacctg attcaggcag aggagaatac tcccgcactt ctgaatgggc gcaacggctg
    25681 gggatcgatc tcaatcaacc gcgagtggtg gctattgttg aggtcgacag cggtcagctt
    25741 ggcgtggaca gcgcaatggc ggagttacaa caactgcaaa acgcgctgac tacgcccgag
    25801 cgtaataatc tggtggcgat tgtctcgcta accgaaatgg tggtgttgaa accggcgttg
    25861 aactcttttg ggcgctggga tgcagaagat catcgtaagc gagttgaaca actgattacc
    25921 cgcatgaaag agtacggcca gctgcgtttt cgcgtttcac tgggcaacta ttttaccggt
    25981 cctagcagta ttgcccgatc ctatcgtacg gcgaaaacga cgatggtggt gggtaaacag
    26041 cggatgccag aaagtcgctg ctatttttat caggatctga tgttacctgt gttactcgac
    26101 agtttgcgtg gcgactggca ggccaacgaa ctggcgcgac cgctggcgcg gctgaaagcg
    26161 atggacaata acggcttgct gcgacgaacg ctggcggcgt ggtttcgcca caatgtgcaa
    26221 ccgctggcaa cgtcaaaggc gttgtttatt catcgtaata ccctggagta tcggcttaat
    26281 cgtatatcgg aactgaccgg gcttgatttg ggcaattttg atgacaggtt gctgctgtat
    26341 gtggcgttac aactggatga agagcggtag attatgcgtt aaggttgagg cggtgctggc
    26401 tcattcccct cagcccgaag gggagagggg aatgcccgag ttaaccgtca attcgggatt
    26461 atttacgcgt tagtttttcc agatcggctt caatctcgct gatcttattg gttacgactg
    26521 actccaggtg acgtaagtcg tcgaggatct tacgcttaag atcaacttcg ctgcggtcac
    26581 gctgacagat ttgatcaagc tcatcaatga tataccgtag attcgggctg atttcctgga
    26641 cttctttata accctgaccc acaccatcag cgacgaccgt tttacgctga cgcggatatt
    26701 taaacttaac gctcttggcg aaaaactcgc ctttgtcttt ctggaaatag attttcagaa
    26761 tatcgttgtt ggcttcctgc cggaggctgt aacgatcaat ttcttcagga ttggtaatac
    26821 ccagactttt cagattgtcg tacatagcgt tacctcaaaa tgagtcagta aattgtgctt
    26881 attttagcat ttggcttggc ccgcccggct cgactgtgat cgcagaaagc ctttttcttc
    26941 cagacttcta tcatggcgta aaaatcaaaa aattacctgc tttattgtgg cgatattcca
    27001 tgggatggat tagcacattt atcttcgttt tgcgaattac atgacagtcc gaaacaagat
    27061 gaaaaagtat ttaaaaaagg gggaggaaga atgagtagga cgaattattc tgagccagaa
    27121 agcatacggg cagcatcatg ctgcccgtat atgtactctt aatcgatggt acgcagcagt
    27181 tcgttaatgc cgactttgcc gcgagttttc gcgtcaactt tcttaacgat aatcgcacag
    27241 tagaggctgt atttgccatc ttttgacggc agattacctg aaacaaccac agaccccgcc
    27301 ggaacgcgac cgtagtggat ttcgccggtt tcacggtcgt aaatacgggt gctctgacca
    27361 atgtatacgc ccatagagat gacggaacct tcttcgacaa tcaccccttc aaccacttca
    27421 gagcgtgcgc caatgaagca gttatcttca ataatggtcg ggttagcctg cagcggctcc
    27481 agcacgccgc cgatgcccac gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa
    27541 gaaccgacgg tcgcccaggt atcaaccatg gtgccttcat caacatatgc gccgatgtta
    27601 acgtaagacg gcatcagcac ggtgttacgg gcaataaacg caccctgacg taccgccgct
    27661 ggtggcacaa cgcggaagcc ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc
    27721 atcggcactt tgtcgaagta gcggctttct gccccttcga tcacctgatt atcattaata
    27781 cggaaagaga gcagcaccgc ttttttcaac cactgatgcg tcacccactg accgtcaatt
    27841 ttttccgcta cacgcagtgc gccggaatcc agcagggcga tcacctgatt taccgcttcg
    27901 cgggtaacgg tgtctgcatt ggctggcgtg atatcggcac ggcgttcaaa agcggtttca
    27961 ataatgttct gtaactgctg cattgttaaa ctcttttcat atcagtaaac acatcaccct
    28021 ttatcgtttg gattgagggc ctctgtcaac cgctgatgca cttcctgctg caactcgtta
    28081 ttaagcgcac gccggtcagc ggtggcaatt atgaataaat cttctactcg ctcgccaatg
    28141 gttgtaattc gggcaccatg aagcgaaatt cccagatcgg caaaaatttt cccgactcgc
    28201 gccagcagtc caggttggtc gagggcgatc agttcgagga acgatttgcg gtcggtatgg
    28261 gtcggcaaaa acgttacttc ggtttcaaca gtaaaatggc gtaatttggc gggttggcga
    28321 cggggctgcg gtggctgcca gctactttgc gtcagcacct gctccagacc aaaccgaata
    28381 acctcatggc gatctgcgga cagcgggctg ccatcgggtt ccagcacgat aaaggtatcc
    28441 atcgccatac cgtcgcgagt ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg
    28501 tctaattcgg cacagacggc ggcaaacaga taggggcggt ccgggctcca gataaaaatc
    28561 tcggtgcctc cacgcgtagc ctgcgggcta agcaatacca gcggtttgct taaatcatgc
    28621 tgtaataaat ggcgggcatg ccaggccagt tgatttgggc tatggcggac aaaatagtta
    28681 gcacgacagc gtgaccaaat ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt
    28741 agtgccagtg cctggagttg gtgatggcga acccgttcgc gcatatccgg cgtgttttgc
    28801 attccgcgtc gtagctgctt ttcggtggca aaatagagtt cgcgcaacag actttgcttc
    28861 cagctattcc acagcgtttc gttggtggcg caaatgtcag ccactgtcag gcataccaga
    28921 tagcgcagac gattttccgt ttgaacttct tcggcaaact gcttgatgac ttcagggtcc
    28981 tgaatatcgc ggcgttgtgc tgtcaccgac atcagcaagt gctggcgaac cagccaggca
    29041 accagctgtg tttcgcgtga gttcagcccg tggagttctg caaaatgcac tacatcctga
    29101 gcaccgagaa tggagtggtc gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg
    29161 atgaaaatca gctcagttga cggcaggcgc ggccagacgt ccacacacaa cggatggcgc
    29221 tggcgcgttt cttcactggc aaaactctcc agtttcagca tcacgcggat agtatgttca
    29281 tccaccgtgt aggcgtggaa cagatcaaac tgcatctgcc cgacgatatg cgaccattgc
    29341 ggcatatacg cgccgagcac gctatggcga tgcattggca atagcccgcg ccgcaccgct
    29401 ccggggtgac gcagaatgct caaaaacagt tttcgtgctt gcggaatatt acacagcggt
    29461 tgttgcagat ggcgacgggc atggcgtaac tggcgcagcg tggtggagta aataccggtg
    29521 atcgcactgt tgcgcaccat ggtgtagaac atacgcaaga tggcttccgg ctggcgcata
    29581 aatagtgttt catcacgcag gtcgattagc gtaccgcgta gctgaaactc atcgtcgagt
    29641 ggacgtggtt tttcgtcggc gggaagggcg aggatggctt catcgaacag ttgcagcagc
    29701 atctggttga gttcactgac gcggcgtgta acgcggaagt aatccttcat catccgctcg
    29761 accggttcgt tgccttcacc actgtaattc agacgctgag cgacgctaag ttggcgatcg
    29821 aataacagac gattatcgta acggctgacg accagatgca gggcaaagcg aatatgccac
    29881 agtatatgca gacattcgtt taattccgcc cgctccgctg aggttaagaa gccaaacccg
    29941 accatttcat ccagcgatgt tgcgccaaaa tgacggcggg ccacccattg cagagtgtga
    30001 atatcgcgca agccgccagg gctacttttg atgtctggtt caaggttgta gctggtgcca
    30061 tggtaacgct gatggcgctg gttctgttct tcaactttcg ctgcgtagaa cttgtcggaa
    30121 ggccagaatc cttcgctaaa aatatgtttt tgcagttcga ggaagagcgc aacatcgcca
    30181 attaataagc gggattcgat taaattggtg gcgacggtta aatccgataa cccttccaac
    30241 atgcactctt caagcgtgcg cacactatga ccgacttcca gctttacatc ccagagcagc
    30301 gttaacagct cgcccacttt ttgcgcctga tcgtccggga gctttttacg gcttaaaatc
    30361 agtaaatcga tgtcagaaag tggatgcagc tcgccacgac cgtagccacc gacggcaacc
    30421 aatgccaggt cggcaatctg gctgaatccc gcttcaatcc ataatcgttg caggagctgg
    30481 tctatgaact cggtacgtgc ctcaatcaac tgttctgcag agatcccatt gtcaaaggca
    30541 tcacccagcc aacgctggaa agtatcgata tgggctttta tcccacagac ggttaattca
    30601 tcacggggcc aggcgcatgg attttgcggt tgaccgggca gggtggagag agcggtgttt
    30661 gcgtactgtt ctggaagggt attcattgtg cgccacccat aagattaaat tatcgcatta
    30721 aaaaagccgg cattcgccgg cttcatctta ttcgtcgtgc gagattatcg ccgggatggt
    30781 gtcatccttg cgtagcgtca aaatttcgca gccgttatca gtcaccacaa tagtatgctc
    30841 atattgtgca gacaagctgc gatctttggt ttttaccgtc cagccatctt tcatggtgcg
    30901 gatctctttt ttacccgcgt tgaccattgg ctcaatggtg aacgtcatcc ccggtttcag
    30961 cacgacgttg gtttcacggg agtcatagtg cagcacctgc ggttcttcat ggaagccgcg
    31021 accaataccg tgtccgcaat attcacgaac gacggagaag ccttctgctt cgacaaattt
    31081 ctgaatcgcc gcaccgattt cgcgcagatt aatgcctggt tttaccatgc gtagcgccag
    31141 gtacaggctt tcttgcgtga tgcggcacag gcgttcgccc atgatggtcg gcttaccgac
    31201 gataaacatt ttcgaggtat cgccgtggaa accatctttg attacggtga catcaatgtt
    31261 aacgatatcg ccatctttca gcagcttagc atcgtccggg ataccgtggc acaccacttc
    31321 attaatagag atgcaaacgg atttcggata gccgtgatag ccgaggcagg cagaaaccgc
    31381 gtgttgttca ttaacaatgt aatcattaca gatgcgatcc agctcgccgg tgctgacgcc
    31441 cggtttaaca tacggttcga tcatctccag cacttcggca gccagtcggc cagcgacgcg
    31501 cattttttcg atatcttctg gggtcttgat tgagatagcc attaattctg tccatcagcg
    31561 tcggtgatac cgacaatata tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg
    31621 agaatcattc tgaatttcgc caaacgtgcc actgaaggtt ttctataata gaaaattcga
    31681 cgtctgatgc tgtacacagc gccaacaatt attggtgtcc acgacgtatt tgtggtataa
    31741 agcgcgccgg acttccgatc catttcgtat acacagactg gacggaagcg acaatctcac
    31801 tttgtgtaac aacacacacg tatcggcaca tattccgggg tgccctttgg ggtcggtaat
    31861 atgggatacg tggaggcata accccaactt ttatatagag gttttaatca tggcaactgt
    31921 ttccatgcgc gacatgctca aggctggtgt tcacttcggt caccagaccc gttactggaa
    31981 cccgaaaatg aagccgttca tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga
    32041 gaaaactgta ccgatgttca acgaagctct ggctgaactg aacaagattg tttctcgcaa
    32101 aggtaaaatc cttttcgttg gtactaaacg cgctgcaagc gaagcggtga aagacgctgc
    32161 tctgagctgc gaccagttct tcgtgaacca tcgctggctg ggcggtatgc tgactaactg
    32221 gaaaaccgtt cgtcagtcca tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg
    32281 tactttcgac aagctgacca agaaagaagc gctgatgcgc actcgtgagc tggagaaact
    32341 ggaaaacagc ctgggcggta tcaaagacat gggcggtctg ccggacgctc tgtttgtaat
    32401 cgatgctgac cacgaacaca ttgctatcaa agaagcaaac aacttgggta ttccggtatt
    32461 tgctatcgtt gataccaact ctgatccgga cggtgttgac ttcgttatcc cgggtaacga
    32521 cgacgcaatc cgtgctgtga ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg
    32581 ccgttctcag gatctggctt cccaggcgga agaaagcttc gtagaagctg agtaataagg
    32641 cttgataact cccccaaaat agttcgagtt gcagaaaggc ggcaagctcg agaattcccg
    32701 ggagcttaca tcagtaagtg accgggatga gcgagcgaag acaacgcatc tgcggcgcga
    32761 aatatgaagg gggagagccc ttatagacca ggtagtacac gtttggttag ggggcctgca
    32821 tatggccccc tttttcactt ttatatctgt gcggtttaat gccgggcaga tcacatctcc
    32881 gaggatttta gaatggctga aattaccgca tccctggtaa aagagctgcg tgagcgtact
    32941 ggcgcaggca tgatggattg caaaaaagca ctgactgaag ctaacggcga catcgagctg
    33001 gcaatcgaaa acatgcgtaa gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt
    33061 gctgctgacg gcgtgatcaa aaccaaaatc gacggcaact acggcatcat tctggaagtt
    33121 aactgccaga ctgacttcgt tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt
    33181 ctggacgcag ctgttgctgg caaaatcact gacgttgaag ttctgaaagc acagttcgaa
    33241 gaagaacgtg ttgcgctggt agcgaaaatt ggtgaaaaca tcaacattcg ccgcgttgct
    33301 gcgctggaag gcgacgttct gggttcttat cagcacggtg cgcgtatcgg cgttctggtt
    33361 gctgctaaag gcgctgacga agagctggtt aaacacatcg ctatgcacgt tgctgcaagc
    33421 aagccagaat tcatcaaacc agaagacgta tccgctgaag tggtagaaaa agaataccag
    33481 gtacagctgg atatcgcgat gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt
    33541 gaaggccgca tgaagaaatt caccggcgaa gtttctctga ccggtcagcc gttcgttatg
    33601 gaaccaagca aaactgttgg tcagctgctg aaagagcata acgctgaagt gactggcttc
    33661 atccgtttcg aagtgggtga aggcatcgag aaagttgaga ctgactttgc agcagaagtt
    33721 gctgcgatgt ccaagcagtc ttaattatca aaaaggagcc gcctgagggc ggcttctttt
    33781 tgtgcccatc ttgtaaattc agctaaccct tgtggggctg cgctgaaaag cgacgtacaa
    33841 tgtcgctagt attaattcat ttcaatcgtt gacagtctca ggaaagaaac atggctacca
    33901 atgcaaaacc cgtctataaa cgcattctgc ttaagttgag tggcgaagct ctgcagggca
    33961 ctgaaggctt cggtattgat gcaagcatac tggatcgtat ggctcaggaa atcaaagaac
    34021 tggttgaact gggtattcag gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg
    34081 ctggtctggc gaaagcgggt atgaaccgcg ttgtgggcga ccacatgggg atgctggcga
    34141 ccgtaatgaa cggcttggca atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc
    34201 tgatgtccgc tattccattg aatggcgtgt gcgacagcta cagctgggca gaagctatca
    34261 gcctgttgcg caacaaccgt gtggtgatcc tctccgccgg tacaggtaac ccgttcttta
    34321 ccaccgactc agcagcttgc ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag
    34381 caaccaaagt tgacggcgtg tttaccgctg atccggcgaa agatccaacc gcaaccatgt
    34441 acgagcaatt gacttacagc gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg
    34501 ccttcacgct ggctcgtgac cataaattac cgattcgtgt tttcaatatg aacaaaccgg
    34561 gtgcgctgcg ccgtgtggta atgggtgaaa aagaagggac tttaatcacg gaataattcc
    34621 cgtgatggat aaataagggt aagattccgc gtaagtatcg cgggggcgta agtctggtta
    34681 taaggcgtta ttgttgcagg cagtttggtc acggccagcg cgcagcaacc ggagcgtaca
    34741 aaagtacgtg aggatggcga gcactgctcg gggccaaaat ggcaaataaa atagcctaat
    34801 aatccagacg attacccgta atatgtttaa tcagggctat acttagcaca cttccactgt
    34861 gtgtgactgt ctggtctgac tgagacaagt tttcaaggat tcgtaacgtg attagcgata
    34921 tcagaaaaga tgctgaagta cgcatggaca aatgcgtaga agcgttcaaa acccaaatca
    34981 gcaaaatacg cacgggtcgt gcttctccca gcctgctgga tggcattgtc gtggaatatt
    35041 acggcacgcc gacgccgctg cgtcagctgg caagcgtaac ggtagaagat tcccgtactc
    35101 tgaaaatcaa cgtgtttgat cgttcaatgt ctccggccgt tgaaaaagcg attatggcct
    35161 ccgatctcgg cctgaacccg aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc
    35221 tgacggaaga acgtcgtaaa gatctgacca aaatcgttcg tggtgaagca gaacaagcgc
    35281 gtgttgcagt acgtaatgtg cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag
    35341 ataaagagat cagcgaagac gacgatcgcc gttctcagga cgatgtacag aaactgactg
    35401 atgctgcaat caagaaaatt gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt
    35461 tctgatttct tgaacgacaa aaacgccgct cagtagatcc ttgcggatcg gctggcggcg
    35521 ttttgctttt tattctgtct caactctgga tgtttcatga agcaactcac cattctgggc
    35581 tcgaccggct cgattggttg cagcacgctg gacgtggtgc gccataatcc cgaacacttc
    35641 cgcgtagttg cgctggtggc aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa
    35701 ttctctcccc gctatgccgt aatggacgat gaagcgagtg cgaaacttct taaaacgatg
    35761 ctacagcaac aggggagccg caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg
    35821 gcagcgcttg aggaggttga tcaggtgatg gcagccattg ttggtgctgc tgggctgtta
    35881 cctacgcttg ctgcgatccg cgcgggtaaa accattttgc tggccaataa agaatcactg
    35941 gttacctgcg gacgtctgtt tatggacgcc gtaaagcaga gcaaagcgca attgttaccg
    36001 gtcgatagcg aacataacgc tatttttcag agtttaccgc aacctatcca gcataatctg
    36061 ggatacgctg accttgagca aaatggcgtg gtgtccattt tacttaccgg gtctggtggc
    36121 cctttccgtg agacgccatt gcgcgatttg gcaacaatga cgcccgatca agcttgccgt
    36181 catccgaact ggtcgatggg acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa
    36241 ggtctggaat acattgaagc gcgttggctg tttaacgcca gcgccagcca gatggaagtg
    36301 ctgattcacc cgcagtcagt gattcactca atggtgcgct atcaggacgg cagtgttctg
    36361 gcgcagctgg gggaaccgga tatgcgtacg ccaattgccc acaccatggc atggccgaat
    36421 cgcgtgaact ctggcgtgaa gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc
    36481 gcaccggatt atgagcgtta tccatgcctg aaactggcga tggaggcgtt cgaacaaggc
    36541 caggcagcga cgacagcatt gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg
    36601 caacaaatcc gctttacgga tatcgctgcg ttgaatttat ccgtactgga aaaaatggat
    36661 atgcgcgaac cacaatgtgt ggacgatgtg ttatctgttg atgcgaacgc gagtgaagtc
    36721 gccagaaaag aggtgatgcg tctcgcaagc tgaggataat ccggctacag agagtcgcgc
    36781 tatttgttag cgtagggctt caatgatata gtctgcgcca tctgatcgta agtagttggc
    36841 tttataaggt caggtatgcc gtggttttac acggcttttt tttgtatagg cttcagtatt
    36901 cctgagtacc gtaaaccctg tcagggaata aaaaacgcgt gatgttgtct gctactcaac
    36961 cacttagcga aaaattgcca gcgcatggct gccgtcatgt tgcgatcatt atggacggca
    37021 atggtcgctg ggcaaaaaag caagggaaga ttcgtgcctt tgggcataaa gccggggcaa
    37081 aatccgtccg ccgggctgtc tcttttgcgg ccaacaacgg tattgaagcg ttaacgctgt
    37141 atgcctttag tagtgaaaac tggaaccgac cagcgcagga agtcagtgcg ttaatggaac
    37201 tgtttgtgtg ggcgctcgat agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc
    37261 gtattattgg cgataccagt cgttttaact cgcgtttgca agaacgtatt cgtaaatctg
    37321 aagcgctaac agccggaaat accggtctga cgctgaatat tgcggcgaac tacggtggac
    37381 gttgggatat agtccaggga gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc
    37441 aaccagatca gatagatgaa gagatgttaa accagcatgt ctgtatgcat gaactggccc
    37501 ctgtagattt agtaattagg actgggggag agcatcgcat tagtaacttt ttgctttggc
    37561 aaattgccta tgccgaactt tactttacag atgttctctg gcccgatttc gatgaacaag
    37621 actttgaagg ggcgttaaat gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc
    37681 ccggtgatga aacagcctga tgggggtcgc ttttgctgaa gtatcgcctg atatctgctt
    37741 ttgtgttaat acccgtcgtc atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca
    37801 ttgtaacgct ggtggtctgc atgctggcag cgtgggaatg gggacagctt agcggtttta
    37861 ccactcgttc gcagcgagta tggttggcgg tgttatgcgg gttattgttg gcgctgatgc
    37921 tttttctgtt gccggaatat caccgaaata ttcatcaacc gctggttgaa atctcacttt
    37981 gggcttcgtt gggttggtgg attgtcgcgc tattgctggt gctgttttac ccaggttccg
    38041 cagcaatctg gcgtaactct aaaacattgc gccttatttt tggcgtgcta accattgttc
    38101 ccttcttctg gggcatgctg gcgttacggg cctggcacta tgacgagaat cattacagtg
    38161 gcgcaatatg gctgctttat gtcatgatcc tggtatgggg cgctgactcc ggcgcatata
    38221 tgtttggcaa attgtttggt aaacataagc tggcaccgaa ggtttctccg ggtaaaacct
    38281 ggcaaggctt tataggtgga ctcgctactg cagcggtaat ctcatggggt tatggcatgt
    38341 gggcgaatct cgacgtcgct cccgtcacct tactcatttg ctctattgtc gcagcgttag
    38401 cctcagtgct cggcgatctg accgagagta tgtttaagcg tgaagcagga attaaggaca
    38461 gcggtcattt aattccagga cacggtggta ttttagatcg tattgatagc ctgacggctg
    38521 cggtaccggt ctttgcttgc ttgttgttac tggtattcag gacgctttaa cggaaggtaa
    38581 tatgctgagt tttctctggg atttggcttc gttcatcgtt gcactgggtg tacttatcac
    38641 cgtgcatgaa tttggtcatt tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt
    38701 ctcaataggg tttggtaagg cgctctggcg gcgaactgat aagctcggca ccgaatatgt
    38761 tatcgccctg atcccgttgg gcggttatgt caaaatgctg gatgagcgcg cagaaccggt
    38821 cgttccggaa ctccgccacc atgccttcaa taataaatct gtcggccaac gagtggcgat
    38881 tattgccgca ggtccggttg caaacttcat ttttgctatc tttgcctact ggctggtttt
    38941 tattattggt gtgcctggcg tacgtccggt ggttggcgaa atagcagcca attcgatagc
    39001 tgcggaagca caaattgcac caggtacgga actaaaagcc gtagatggta tcgaaacgcc
    39061 tgattgggat gccgtgcgtt tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat
    39121 tacagtagcg ccatttggca gcgaccaacg gcgggatgta aagctcgatt tacgtcactg
    39181 ggcgtttgag cctgataaag aagatccggt atcttcgctg gggattcgtc ctcgtgggcc
    39241 gcaaattgaa cctgtactgg aaaatgtgca gccaaactcg gcggcaagca aggcaggttt
    39301 gcaagcaggc gacaggatcg ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt
    39361 tgtgatgctt gtccgggata acccgggtaa atccttagcg ttagaaatcg aaaggcaggg
    39421 gagtcccttg tctttgacat taatcccgga gagtaaaccg ggtaatggta aagcgattgg
    39481 ttttgtcggt attgagccga aagtcattcc tttgccagat gagtataaag ttgtacgcca
    39541 gtatgggccg ttcaacgcca tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct
    39601 gacggtcagt atgctgggaa aattgatcac cggtgatgtg aaactgaaca acctcagtgg
    39661 gccgatttct atcgccaagg gggctgggat gacagcggaa ctcggggtag tttattacct
    39721 gccgtttctt gcgcttatta gcgtgaactt agggataatt aacctgtttc cgttgcccgt
    39781 acttgacggg gggcatctgc tgttccttgc gatcgaaaag atcaagggcg gaccggtatc
    39841 cgagcgggtt caagactttt gttatcgcat tggctcgatt ctgctggtgc tgttaatggg
    39901 gcttgcactt ttcaatgatt tctctcggtt atgagagtta gttaggaaga acgcataata
    39961 acgatggcga tgaaaaagtt gctcatagcg tcgctgctgt ttagcagcgc caccgtatac
    40021 ggtgctgaag ggttcgtagt gaaagatatt catttcgaag gccttcagcg tgtcgccgtt
    40081 ggtgcggccc tcctcagtat gccggtgcgt acaggcgaca cggttaatga tgaagatatc
    40141 agtaatacca ttcgcgctct gtttgctacc ggcaactttg aggatgttcg cgtccttcgt
    40201 gatggtgata cccttctggt tcaggtaaaa gaacgtccga ccattgccag cattactttc
    40261 tccggtaaca aatcggtgaa agatgacatg ctaaagcaaa acctcgaggc ttctggtgtg
    40321 cgtgtgggcg aatccctcga tcgcaccacc attgccgata tcgagaaagg tctggaagac
    40381 ttctactaca gcgtcggtaa atatagcgcc agcgttaaag ctgtcgtgac cccgctgccg
    40441 cgcaaccgtg ttgacctaaa actggtgttc cgggaaggtg tgtcagctga aatccagcaa
    40501 attaacattg ttggtaacca tgctttcacc accgacgaac tgatctctca tttccaactg
    40561 cgtgacgaag tgccgtggtg gaatgtggta ggcgatcgta aataccagaa acagaaactg
    40621 gcgggcgacc ttgaaaccct gcgcagctac tatctggacc gcggttatgc ccgtttcaac
    40681 atcgactcta cccaggtcag tctgacgcca gataaaaaag gtatttacgt cacggtgaac
    40741 atcaccgaag gcgatcagta caagctttct ggcgttgaag tgagcggcaa ccttgccggg
    40801 cactccgctg aaattgagca gctgactaag atcgagccgg gcgagctgta taacggcact
    40861 aaagtgacca aaatggaaga tgacattaaa aagcttctcg gtcgctatgg ttatgcctat
    40921 ccgcgcgtac agtcgatgcc tgaaattaac gatgccgaca aaaccgttaa attacgcgtg
    40981 aacgttgatg cgggtaaccg tttctacgtg cgtaagatcc gttttgaagg taacgatacc
    41041 tcgaaagatg ccgtcctgcg tcgcgaaatg cgtcagatgg aaggtgcatg gttggggagc
    41101 gatctggtcg atcagggtaa ggagcgtctg aatcgtctgg gcttctttga aactgtcgat
    41161 actgataccc aacgtgttcc gggtagcccg gaccaggttg atgtagtcta caaggtgaaa
    41221 gaacgtaaca ctggtagctt caacttcggg attggttacg gtactgaaag tggcgtgagc
    41281 ttccaggctg gcgtgcaaca ggataactgg ttaggtacag gttatgctgt tggtatcaac
    41341 gggaccaaaa acgattacca gacctatgct gaactgtcgg taaccaaccc gtacttcacc
    41401 gtagatggcg taagcctcgg tggtcgtctc ttctataatg acttccaggc agatgacgcc
    41461 gacctgtccg actataccaa caagagttat ggtacagacg tgacgttggg cttcccgatt
    41521 aacgaatata actcgctgcg tgcaggtctg ggttatgtac ataactccct gtccaacatg
    41581 cagcctcagg ttgcgatgtg gcgttatctg tactctatgg gtgaacatcc gagcacttct
    41641 gatcaggata acagcttcaa aacggacgac ttcacgttca actatggttg gacctataac
    41701 aagcttgacc gtggttactt cccgacagat ggttcacgtg tcaacctgac cggtaaagtg
    41761 accattcctg gatcggataa cgaatactac aaagtgacgt tagacacggc gacttatgtg
    41821 ccgatcgatg acgatcacaa atgggttgtt ctgggtcgta cccgctgggg ttatggtgat
    41881 ggtttaggcg gcaaagagat gccgttctac gagaacttct atgccggtgg ttccagcacc
    41941 gtgcgtggct tccagtccaa taccattggt ccgaaagcag tttacttccc gcatcaggcc
    42001 agtaattatg atccggacta tgattacgaa tgtgcgactc aggacggcgc gaaagacctg
    42061 tgtaaatcgg atgatgctgt aggcggtaac gccatggcgg ttgccagcct cgagttcatc
    42121 accccgacgc cgtttattag cgataagtat gctaactcgg ttcgtacttc attcttctgg
    42181 gatatgggta ccgtttggga tacaaactgg gattccagcc aatattctgg atatccggac
    42241 tatagtgatc caagcaatat ccgtatgtct gcgggtatcg cattacaatg gatgtcccca
    42301 ttggggccgt tggtgttctc ctacgcccag ccgttcaaaa agtacgatgg agacaaggca
    42361 gaacagttcc agtttaacat cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt
    42421 ggtagtgtag cgatgacttt aggcgatcaa tataagatcg ccgggccacg caaagaactg
    42481 caccctccgg tgcaaatggg atggtaagga gtttattgtg aaaaagtggt tattagctgc
    42541 aggtcttggt ttagcactgg caacttctgc tcaggcggct gacaaaattg caatcgtcaa
    42601 tatgggcagc ctgttccagc aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa
    42661 tgagttcaaa ggccgtgcca gcgaactgca gcgtatggaa accgatctgc aggctaaaat
    42721 gaaaaaactg cagtccatga aagcgggcag cgatcgcacc aaactggaaa aagacgtgat
    42781 ggctcagcgc cagacttttg ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg
    42841 ttccaacgaa gaacgcggca aactggttac tcgtatccag actgctgtga aatccgttgc
    42901 caacagccag gatatcgatc tggttgttga tgcaaacgct gttgcttaca acagcagcga
    42961 tgtaaaagac atcactgccg acgtactgaa acaggttaaa taagtaatgc cttcaattcg
    43021 actggctgat ttagcgcagc agttggatgc agaactacac ggtgatggcg atatcgtcat
    43081 caccggcgtt gcgtccatgc aatctgcaca aacaggtcac attacgttca tggttaaccc
    43141 aaaataccgt gagcatttag gcttgtgcca agcgtccgcg gttgtcatga cccaggacga
    43201 tcttcctttc gcgaaaagtg ccgcgctggt agtgaagaat ccctacctga cttacgcgcg
    43261 catggcgcaa attttagata ccacgccgca gcccgcgcag aacattgcac ccagtgcggt
    43321 gatcgacgcg acggcgaagc tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga
    43381 gtccggcgtt gaactgggcg ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa
    43441 cagcaaaatc ggtgcaggtt cgcgtctctg ggcgaacgta accatttacc atgagatcca
    43501 gatcggtcag aattgcctga tccagtccgg aacagtggta ggcgcagacg gctttggtta
    43561 tgccaacgat cgtggtaact gggtgaagat cccacagatt ggtcgcgtaa ttattggcga
    43621 tcgcgtggag atcggtgcct gcacaaccat cgatcgcggc gcgctggatg acactgttat
    43681 tggcaatggc gtgatcattg ataaccagtg ccagattgca cataacgtcg tgattggcga
    43741 caatacggcg gttgccggtg gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg
    43801 catgatcggc ggagccagcg taatcaacgg gcatatggaa atatgcgaca aagtgacggt
    43861 tacgggcatg ggtatggtga tgcgtcccat cactgaacca ggcgtctatt cctcaggcat
    43921 tccgctgcaa cccaacaaag tctggcgcaa aaccgctgca ctggtgatga acattgatga
    43981 catgagcaag cgtctgaaat cgcttgagcg caaggttaat caacaagact aacgttccat
    44041 cttttgttcg ccaaacttta cggcctgtct cattcttacg attgcggcag gccgtgttat
    44101 tattgtcgtt tcttatattt tgacaggaag agtatcttga ctactaacac tcatactctg
    44161 cagattgaag agattttaga acttctgccg caccgtttcc cgttcttact ggtggatcgc
    44221 gtgctggatt ttgaagaagg tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag
    44281 ccattcttcc agggccattt ccctggaaaa ccgattttcc cgggtgtgct gattctggaa
    44341 gcaatggcac aggcaacagg tattctggcg tttaaaagcg taggaaaact ggaaccgggt
    44401 gagctgtact acttcgctgg tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc
    44461 gatcaaatga tcatggaagt cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa
    44521 ggggttgctc tggtcgatgg taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc
    44581 cgggaggcct gatacgtgat tgataaatcc gcctttgtgc atccaaccgc cattgtggaa
    44641 gagggcgcgt caattggcgc gaacgcacac attggtcctt tttgtatcgt tggaccccat
    44701 gtcgaaattg gtgagggtac cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa
    44761 attggccgcg ataatgagat ttatcagttt gcctccatcg gcgaagttaa ccaggatttg
    44821 aaatatgctg gcgaaccgac ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc
    44881 gtcaccattc atcgtggcac agtccagggc ggtggattga cgaaggtggg cagcgacaac
    44941 ttactgatga tcaacgcgca cattgcgcac gattgtacgg taggtaaccg ctgtattctc
    45001 gccaacaacg caacgctggc gggtcacgta tcggttgacg acttcgcgat catcggcggc
    45061 atgaccgcag tccatcagtt ctgcatcatt ggtgcgcatg tgatggttgg cggctgctcc
    45121 ggtgtggcgc aggacgtccc tccttatgtc attgcgcagg gtaaccacgc aacgccgttc
    45181 ggtgtcaata tcgaagggct gaagcgccgc ggattcagcc gtgaggcgat taccgctatc
    45241 cgcaatgcgt ataagctaat ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa
    45301 attgctgaac tggcggaaac atatccggaa gtgaaagcct ttaccgattt ctttgcacgc
    45361 tcaacgcgcg gtctgattcg ttaatgactg accagcgtcc attaacgatt gccctggtcg
    45421 ccggagaaac ctccggcgat atcctggggg ccggtttaat ccgcgctctg aaagaacgtg
    45481 tgcccaacgc ccgctttgtt ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag
    45541 cctggtacga aatggaagaa ctggcggtga tgggcattgt tgaagtgctc ggtcgtctgc
    45601 gtcgcttact gcatattcgt gccgatctga caaagcgttt tggcgaactg aagccagatg
    45661 tttttgttgg tattgatgcg cccgacttca atattactct tgaaggtaac ctcaaaaagc
    45721 agggtatcaa aaccattcat tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg
    45781 ttttcaaaat aggcagagcc accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt
    45841 tttatgacaa atacaatgta ccgtgccgct ttatcggtca taccatggct gatgccatgc
    45901 cgttagatcc agataaaaat ggtgcccgtg atgtgctggg gatcccttac gatgcccact
    45961 gtctggcatt gttgccgggc agccgtggtg cagaagtcga aatgcttagt gccgatttct
    46021 tgaaaactgc ccagcttttg cgccagacat atccggatct cgaaatcgtg gtgccgctgg
    46081 tgaatgccaa acgccgcgag cagtttgaac gcatcaaagc tgaagtcgcg ccagacctgt
    46141 cggttcattt gctggatgga atgggccgtg aggcgatggt cgccagcgat gcggcactac
    46201 tggcgtcggg gacggcagcc ctggagtgta tgctggcgaa atgcccgatg gtggtgggat
    46261 atcgcatgaa gccttttacc ttctggttgg cgaagcggct ggtgaaaact gattatgtct
    46321 cgctgccaaa tctgctggcg ggcagagagt tagtcaaaga gttattgcag gaagagtgtg
    46381 agccgcaaaa actggctgcg gcgctgttac cgctgttggc gaacgggaaa accagccacg
    46441 cgatgcacga taccttccgt gaactgcatc agcagatccg ctgcaatgcc gatgagcagg
    46501 cggcacaagc cgttctggag ttagcacaat gatcgaattt gtttatccgc acacgcagct
    46561 ggttgcgggt gtggatgaag tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc
    46621 ggtgatcctt gacccggcgc gcccgattgc cgggctgaat gattccaaaa agctgagcga
    46681 aaaacgccgt ctggcgctct atgaagagat caaagagaaa gcgttgagct ggagtctggg
    46741 ccgcgcggaa ccccacgaaa tcgacgagct gaacattctt catgcgacca tgctggcgat
    46801 gcagcgtgcc gtcgctgggc tgcatattgc gccggaatat gtgttgattg atggtaaccg
    46861 ctgcccgaaa ttaccgatgc ctgcgatggc tgtggtgaaa ggggatagcc gcgtaccaga
    46921 aatcagtgcc gcgtctatcc tagcgaaagt gacgcgtgac gccgaaatgg cggcgctgga
    46981 tattgttttc ccgcaatatg gttttgccca acataaaggg tatccaaccg cttttcatct
    47041 ggaaaaactg gctgaatatg gcgcgactga acatcatcgt cgcagctttg ggcctgtcaa
    47101 acgcgcactg ggacttgcgt cctgattctt gtgtcgagat taagtaaacc ggaatctgaa
    47161 gatgtctgaa ccacgtttcg tacacctgcg ggtgcacagc gactactcga tgatcgatgg
    47221 cttggccaaa accgcaccgc tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc
    47281 gatcaccgat ttcaccaacc tttgcggtct ggtgaagttc tacggagcgg gacatggcgc
    47341 agggattaag cctatcgtcg gggcagattt taatgtccag tgcgacctgc tgggtgatga
    47401 gttaacccac ctgacggtac tggcggcgaa caataccggc tatcagaatc tggcgttgct
    47461 gatctcaaaa gcgtatcagc gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg
    47521 gcttatcgaa ttaaacgaag ggttgatcct tctttccggc gggcgcatgg gcgacgtcgg
    47581 acgcagtctt ttgcgtggta acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga
    47641 acacttcccg gatcgctatt ttctcgagct gatccgcacc ggcaggccgg atgaagaaag
    47701 ctatctgcac gcggcggtgg aactggcgga agcgcgcggt ttgcccgtcg tggcgactaa
    47761 cgatgtgcgc tttatcgaca gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca
    47821 cgacggcttt accctcgacg atcctaaacg cccgcgtaac tattcgccgc agcaatatat
    47881 gcgtagcgaa gaggagatgt gtgagctgtt tgccgacatc ccagaagccc ttgccaacac
    47941 cgttgagatc gccaaacgct gtaacgtaac tgtgcgtctt ggtgaatact tcctgccgca
    48001 gttcccgacc ggggacatga gcaccgaaga ttatctggtc aagcgtgcaa aagaaggcct
    48061 ggaagagcgt ctggcatttt tattccctga cgaggaagaa cgtcttaaac gccgcccgga
    48121 atatgacgaa cgtctggaga ctgaacttca ggttatcaac cagatgggtt tcccgggcta
    48181 cttcctcatc gttatggaat ttatccagtg gtcgaaagat aacggcgtac cggtaggacc
    48241 aggccgtggc tccggtgcgg gttcactggt ggcctacgcg ctgaaaatca ccgacctcga
    48301 tccgctggaa tttgacctgc tgttcgaacg tttccttaac ccggaacgtg tctccatgcc
    48361 tgacttcgac gttgacttct gtatggagaa acgcgatcag gttatcgaac acgtggcgga
    48421 catgtacggt cgtgatgcgg tatcgcagat tatcaccttc ggtacgatgg cggcgaaagc
    48481 ggtgatccgc gacgtaggcc gcgtgctggg gcatccgtac ggctttgtcg accgtatctc
    48541 gaaactgatc ccgcccgatc cggggatgac gctggcgaaa gcgtttgaag ccgagccgca
    48601 gctgccggaa atctacgaag cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa
    48661 actggaaggg gtcacccgta acgccggtaa gcacgccggt ggggtggtta tcgcgccgac
    48721 caaaattacc gattttgcgc cgctttattg cgatgaagag ggcaaacatc cggtcaccca
    48781 gtttgataaa agcgacgttg aatacgccgg actggtgaag ttcgacttcc ttggtttgcg
    48841 tacgctcacc atcatcaact gggcgctgga gatgatcaac aagcggcggg cgaagaatgg
    48901 cgagccaccg ctggatatcg ccgcgatccc gctggatgac aagaaaagct tcgacatgct
    48961 gcaacgctcg gaaaccacgg cggtattcca gcttgaatcg cgcggcatga aggacctgat
    49021 caagcgtctg caacctgact gcttcgaaga tatgatcgca ctggtggcac tgttccgccc
    49081 tggtccgttg cagtcaggga tggtggataa ctttatcgac cgtaaacatg gtcgtgaaga
    49141 gatctcctat ccggacgtgc agtggcagca tgaaagcctg aaatcggtac tggagccaac
    49201 ctacggcatt atcttgtatc aggaacaggt catgcagatt gcccaggtgc tttctggtta
    49261 taccctcggt ggcgcggata tgctgcgccg tgcgatgggt aagaaaaagc cggaagagat
    49321 ggctaagcag cgttctgtat ttgctgaagg tgcagaaaag aacggaatca acgccgaact
    49381 ggcgatgaaa atcttcgacc tggtggagaa attcgcgggt tacggattta acaaatcgca
    49441 ctctgcggcc tatgctttgg tgtcatatca aacgttatgg ctgaaagcgc actatccggc
    49501 ggagtttatg gcggcggtaa tgaccgccga tatggacaac accgagaagg tggtgggcct
    49561 ggtggatgag tgctggcgga tggggctgaa aatcctgcca ccagatataa actccggtct
    49621 ttaccatttc cacgtcaacg acgacggcga aatcgtgtat ggtattggcg ccatcaaagg
    49681 ggtaggtgaa ggtccgattg aggccatcat cgaagcccgt aataaaggcg gctacttccg
    49741 cgaactgttt gatctctgcg cccgtaccga caccaaaaag ttaaaccggc gggtgctgga
    49801 aaaactgatc atgtccgggg cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa
    49861 ctcgctgggt gatgcgttaa aagcggcaga tcaacacgcg aaagcggaag ctatcggtca
    49921 ggccgatatg ttcggcgtgc tggccgaaga gccggaacaa attgaacaat cctacgccag
    49981 ctgccaaccg tggccggagc aggtggtatt agatggggaa cgtgaaacgt taggcctgta
    50041 cctgaccgga caccctatca accagtattt aaaagagatt gagcgttatg tcggaggcgt
    50101 aaggctgaaa gacatgcacc cgacagaacg tggtaaagtc atcacagctg cggggctcgt
    50161 tgttgccgcg cgggttatgg tcaccaagcg cggcaatcgt atcggtatct gcacgctgga
    50221 tgaccgttcc gggcggctgg aagtgatgtt gtttactgac gccctggata aataccagca
    50281 attgctggaa aaagaccgca tacttatcgt cagcggacag gtcagctttg atgacttcag
    50341 cggtgggctt aaaatgaccg ctcgcgaagt gatggatatt gacgaagccc gggaaaaata
    50401 tgctcgcggg cttgctatct cgctgacgga caggcaaatt gatgaccagc ttttaaaccg
    50461 actccgtcag tctctggaac cccaccgctc tgggacaatt ccagtacatc tctactatca
    50521 gagggcggat gcacgcgcgc ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga
    50581 tcgtttatta aacgatctcc gtggcctcat tggttcggag caggtggaac tggagtttga
    50641 ctaatacagg aatactatga gtctgaattt ccttgatttt gaacagccga ttgcagagct
    50701 ggaagcgaaa atcgattctc tgactgcggt tagccgtcag gatgagaaac tggatattaa
    50761 catcgatgaa gaagtgcatc gtctgcgtga aaaaagcgta gaactgacgc gtaaaatctt
    50821 cgccgatctc ggtgcatggc agattgcgca actggcacgc catccgcagc gtccttatac
    50881 cctagattac gttcgcctgg catttgatga atttgacgaa ctggctggcg accgtgcgta
    50941 tgcagacgat aaagctatcg tcggtggtat cgcccgtctc gatggtcgtc cggtgatgat
    51001 cattggtcat caaaaaggtc gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc
    51061 agcgccagaa ggttaccgca aagcactgcg tctgatgcaa atggctgaac gctttaagat
    51121 gcctatcatc acctttatcg acaccccggg ggcttatcct ggcgtgggcg cagaagagcg
    51181 tggtcagtct gaagccattg cacgcaacct gcgtgaaatg tctcgccttg gcgtaccggt
    51241 agtttgtacg gttatcggtg aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga
    51301 taaagtgaat atgctgcaat acagcaccta ttccgttatc tcgccggaag gttgtgcgtc
    51361 cattctgtgg aagagcgccg acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc
    51421 tccgcgtctg aaagagctga aactgatcga ctccatcatc ccggaaccgc tgggcggcgc
    51481 tcaccgtaac ccggaagcga tggcggcatc gttgaaagcg caactgctgg cggatctggc
    51541 cgatctcgat gtgttaagca ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag
    51601 ctacggttac gcgtaattcg caaaagttct gaaaaagggt cacttcggtg gccctttttt
    51661 atcgccacgg tttgagcagg ctatgattaa ggaaggattt tccaggagga acacatgaac
    51721 atcattgcca ttatgggacc gcatggcgtc ttttataaag atgagcccat caaagaactg
    51781 gagtcggcgc tggtggcgca aggctttcag attatctggc cacaaaacag cgttgatttg
    51841 ctgaaattta tcgaacataa ccctcgaatt tgcggcgtga tttttgactg ggatgagtac
    51901 agtctcgatt tatgcagcga tatcaatcaa cttaatgaat atctcccgct ttatgccttc
    51961 atcaacaccc actcgacgat ggatgtcagc gtgcaggata tgcggatggc gctctggttt
    52021 tttgaatatg cgctggggca ggcggaagat atcgccattc gtatgcgtca gtacaccaac
    52081 gaatatcttg ataacattac gccgccgttc acgaaagcct tgtttaccta cgtcaaagag
    52141 cggaagtaca ccttttgtac gccggggcat atgggcggca ccgcatatca aaaaagcccg
    52201 gttggctgtc tgttttatga ttttttcggc gggaataccc ttaaggctga tgtctctatt
    52261 tcggtcaccg agcttggttc gttgcttgac cacaccgggc cacacctgga agcggaagag
    52321 tacatcgcgc ggacttttgg cgcggaacag agttatatcg ttaccaacgg aacatcgacg
    52381 tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca gtacgctgtt gatcgaccgc
    52441 aactgccata aatcactggc gcatctgttg atgatgaacg atgtagtgcc agtctggctg
    52501 aaaccgacgc gtaatgcgtt ggggattctg ggggggatcc cgcgccgtga atttactcgc
    52561 gacagcatcg aagagaaagt cgctgccact acgcaagcac aatggccggt tcatgcggtg
    52621 atcaccaact ccacctatga tggtttgctc tacaacaccg actggatcaa acagacgctg
    52681 gatgtcccgt cgatccactt cgattccgcc tgggtgccgt atactcattt tcatccaatc
    52741 taccagggta aaagtggtat gagcggcgag cgtgttgcgg gaaaagtttt cttcgaaacg
    52801 caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt cactgatcca cattaaaggc
    52861 gagtatgacg aagaggcgtt taacgaagcc tttatgatgc ataccaccac ctcgcccagt
    52921 tatcccattg ttgcttcggt tgagacggcg gcggcgatgc tgcgtggtaa tccgggcaaa
    52981 cggctgatta accgttcggt ggagcgggcg ctgcattttc gcaaagaggt ccagcggctg
    53041 cgggaagagt ctgacggttg gtttttcgat atctggcaac cgccgcaggt ggatgaagcc
    53101 gaatgctggc ccgttgcgcc tggcgaacaa tggcacggct ttaacgatgc ggatgccgat
    53161 catatgtttc tcgatccggt taaagtcact attttgacac cggggatgga cgaacagggc
    53221 aatatgagtg aggaggggat cccggcggcg ctggtagcaa aattcctcga cgaacgtggg
    53281 atcgtagtag agaaaaccgg tccttataac ctgctgtttc tctttagtat tggcatcgat
    53341 aaaaccaaag caatgggatt attgcgtggg ttgacggaat ttaagcgctc ttacgatctc
    53401 aacctgcgga tcaaaaatat gctgcccgat ctctatgctg aagatcccga tttctaccgc
    53461 aatatgcgta ttcaggatct ggcgcaaggg atccataagc tgattcgtaa acacgatctt
    53521 tccggtttga tgttgcgggc attcgatact ttgccggaaa tgatcatgac gccacatcag
    53581 gcatggcaga gacagattaa aggtgaagta gaaaccattg cgctggaaca actggtcggt
    53641 agagtatcgg caaatatgat cctgccttat ccaccgggcg taccgctgtt gatgccagga
    53701 gaaatgctga ccaaagagag ccgcacggta ctcgattttc tactgatgct ttgttccgta
    53761 gggcaacatt accccggttt tgaaacggat attcacggcg cgaaacagga cgaagacggc
    53821 gtttaccgcg tacgagtcct aaaaatggcg ggataacttg ccagagcggc ttccgggcga
    53881 gtaacgtgct gttaacaaat aaaggagacg ttatgctggg tttaaaacag gttcaccata
    53941 ttgcgattat tgcgacggat tatgcggtga gcaaagcttt ctactgcgat attcttggtt
    54001 tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc gtggaaaggg gatttggcgc
    54061 ttaatgggca atatgtgatt gagcttttct cttttccgtt cccgccggag cgccccagcc
    54121 gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt tgatgacatc gatgcggcag
    54181 tggcgcacct tgaaagccat aacgtgaagt gtgaagccat ccgtgtcgat ccatacacgc
    54241 aaaaacgctt caccttcttt aacgatccgg acgggctgcc gttggaactg tatgagcagt
    54301 aaggcttgtc atcgccgcat ttgcccggta acgtgccggg cattgctact gtaaaatcgc
    54361 accatcatga cactcacgct caatagacca cttctcacct cacgccagat tctggtggcc
    54421 tttagcggcg ggcttgactc caccgttctg ctgcatcagt tggtgcagtg gcggacggaa
    54481 aatccgggcg tcactctgcg cgctattcat gtgcatcacg gtttaagtgc caatgccgat
    54541 gcctgggtta cgcattgcga aaacgtctgc caacagtggc aggtgccgct ggtggtcgaa
    54601 cgcgtacaac ttgcgcaaga ggggctgggg attgaggccc aggcgcggca ggcgcgttat
    54661 caggcatttg cccgtaccct attgcccggc gaagtgttgg tcaccgcgca acatctcgac
    54721 gatcaatgtg aaacctttct gctggcgcta aaacgcggta gcggccctgc cgggctttcg
    54781 gctatggcgg aagtttcgga gtttgctgga acacggctta tccgcccgtt gcttgcccgc
    54841 acgcgggggg aactggagca gtgggcattg gcgcatggtt tacgctggat tgaagacgaa
    54901 agtaatcagg acgactcata cgatcgtaac tttctgcgcc tgcgcgtagt gccgttattg
    54961 cagcagcgtt ggccgcattt tgcggaagca acggcccgca gcgccacact ttgtgctgaa
    55021 caagagagtc tgttggatga actgctggcg gatgatttag cacactgcca aacgtcgcag
    55081 gggacgctgc agattgcgcc aatgctggcg atgagtgatg cccgtcgcgc ggcgattatt
    55141 cgccgctggc tggcagggca gaatgcaccg atgccttccc gcgacgcgct ggtgcgcatc
    55201 tggcaggaag tggcgctggc gcgggaagat gcttccccct gtttacgttt gggcgccttt
    55261 gaaatccgcc gctatcagtc gcaactgtgg tggattaaat ccgtcaccgg gcaaagcgaa
    55321 accattgtgc tgtggcagac gtggctacaa ccgctggaat taccggcggg gctgggaacc
    55381 gtacagctta ctgcgggagg cgatattcgc cctccgcgtg cagacgaagc ggtcagcgtg
    55441 cgtttcaaag cgccaggatt gctgcatatt gtcgggcgca acggcggacg taagctgaag
    55501 aaaatctggc aagagttagg cgtgccgccg tggctacgtg acaccacgcc actgctgttt
    55561 tatggagaaa cgctgattgc ggcggcaggg gtatttgtga cgcaagaagg tgtggctgaa
    55621 ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta gttaagtgaa agccggataa
    55681 gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat tcgcttacca ccaccgtacc
    55741 gatttcaggg tggctaaagc tggtaatttt atccagacgc agctcgcggg tttcgccagc
    55801 ggcctcgacg accagatact ccacattttt gcgggagact aaatcactgg cttttgcctg
    55861 caatttttcg ccatctttca gctcaagtgt cagcattaaa tgatgctggc aggcgagctc
    55921 aagattatcg taatcatcac aattaattgg ttgatacgta tcattcattg acataatcgc
    55981 tcaccagtaa gtttgccgca gcgtatgctg ctttttccct gacagcctca gaaagggcgt
    56041 cgtcggcagc catttcattc agcactttca aaacgcagcc cagcgcgtcc ggaacgtatc
    56101 ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat taactcacaa tatttttcca
    56161 catgccctcc tgtcagcact ctgacttaac cgtggatgca agtctaagcc tacgaagata
    56221 aactctgttt cgcaaggtga ctataccaca ctcatttctg caatatcagc gccgcaactg
    56281 cacgtattcc gttacaatgg cctcctgatt cgaaaggagt tttcttatgg cgcttaaagc
    56341 gacaatttat aaagcgacgg ttaatgtggc cgatctcgac cgcaaccagt ttctcgatgc
    56401 ctctctgacg ctggcgcgcc acccttcaga aacccaggag cgtatgatgc tgcgcttgct
    56461 ggcgtggctg aaatatgctg atgaacgtct gcaatttacc cgtggtttgt gtgccgatga
    56521 tgagccggaa gcgtggctgc gtaacgatca cctgggcatt gatttgtgga ttgagctggg
    56581 gctgccggat gagcgacgaa ttaagaaagc ctgcacccag gccgcagaag tggcgctgtt
    56641 tacctataat agtcgggcgg cgcaaatctg gtggcagcaa aatcagagca aatgtgtgca
    56701 gtttgccaat ctttccgtct ggtatctgga cgatgaacaa ctggcgaaag taagcgcctt
    56761 tgccgatcgt accatgacgc tgcaggcaac gattcaggat ggtgttatct ggttatcgga
    56821 tgataagaat aatctggaag ttaacttaac cgcctggcaa caaccttcat gattgtgatt
    56881 tcccgacatg ttgctattcc cgatggtgag cttgagatca ccgccattcg tgcgcagggc
    56941 gctggcgggc agcatgttaa taagacctca acggctattc atctgcgttt tgacattcgg
    57001 gcgcccagcc tgccagagta ttacaaagag cgtctgctcg ccgccagcca tcatttgatc
    57061 agcagtgatg gcgtgattgt cattaaggca caggaatacc gcagtcagga actgaaccgc
    57121 gaagccgctc tggcccggct ggtggcagtg attaaagatt taacaaaaga acaaaaagcc
    57181 cgacgaccca cgcggcccac ccgtgcatcg aaagagcgca ggctggcatc gaaagcacaa
    57241 aaatcaagcg tgaaggcgat gcgcggcaaa gtacgcagcg gtcgggaata aaaagaagga
    57301 atggatggtg aaaaaagcga tagtgacagc gatggctgta atcagcctct ttactctgat
    57361 gggatgtaat aatcgggccg aagtcgatac gctttctccg gcgcaggctg ccgaactgaa
    57421 accgatgccg caaagttggc gcggcgtgct accgtgtgcc gattgcgaag gaatcgaaat
    57481 ctctctgttc ctcgaaaaag acggaacatg ggtgatgaat gagcgttatc tcggtgctcg
    57541 tgaagaacct tcctccttcg cttcctacgg tacatgggcg cgaaccgctg acaagctggt
    57601 attaaccgac agcaaaggtg aaaagtcata ttatcgggcg aaaggcgatg cgctggagat
    57661 gctcgatcgt gaaggcaatc caattgaatc gcagttcaac tatacgctgg aaccggcaca
    57721 atccagttta cctatgacgc cgatgaccct gcgaggcatg tatttttata tggctgatgc
    57781 ggcgaccttc actgattgcg cgaccggaaa acgtttcatg gtagcgaata acgcagagct
    57841 ggagcgtagc tacctggctg cgcgcggtca cagtgaaaaa ccggtgttac tgtcagtaga
    57901 aggtcacttt acgcttgagg ctaatccgga tactggtgcg ccaaccaaag tattagcgcc
    57961 cgatacggcg ggtaaatttt acccgaataa ggattgcagt agtttggggc agtaaacctg
    58021 cttaaggcag aaacaaacgc aaaattgcct gatgcgctgc gcttatcagg cctacagcat
    58081 ataatgcaac attttgaatt agcacgattt tgtaggccgg ataaggcgtt tacgccgcat
    58141 ccggcatttt gtgcacgagg ctggctttat cactgagtca gcccaacaaa gcgacccgcc
    58201 ttgatataaa tcccaggctt caggtgccca acatactgta actgcgtttc tggcttaaac
    58261 gcagagacat caccaatacg catatgcatc agatcggcag ggctaatcca gcccgattgc
    58321 gccagtgtaa gtggatgccc ggctttggca aatgcatcag tgacaaattc cgaacaaaac
    58381 cacgactttt tgtctccttc gcacacactg cttaactgcg ctttcgccag gtcgctgacg
    58441 cactgttggc gaaaatcctc ggagaacgga ttcagtgagc acatctggcg agtcaccata
    58501 aagggaataa attcgacaat gctgcgatag ttataaccgc tatctttgat tttattggca
    58561 aacgcggtga tatctgtggc ttgttgcggg gtaagatccg ggactcgtaa gacgaaaagc
    58621 ttatcactat gcttcatcgc ttttttaagg gaaacaatct ggacgccagc gcctgtcgct
    58681 tctgcaacgt tattatcacc cagaaagatt gcaacgtgac tcacagagga agtgctgaag
    58741 acgcggattc caaatgaggt taccccaagg cttgaggaga acagcaaatc gccgggtttg
    58801 agatcaggtg ccgttatttc ttttattgat tgttcggtga aagagctttg atgctggaat
    58861 ttgacagccc atgtttttgc ctcggcatct actgctgtgg ctgatgggtc tggctggctg
    58921 atatcaacgg tacaagccga aagtaaaaga aagctgggga gaagcaggcg gcagtacgcc
    58981 tttggtttat ccattttata caatccatgt aaaaaagggc cctgaaattc aggacccttt
    59041 ctggcatcag cctttaatct gtttcaccag atattcgacg atgtcaccag tcttaattaa
    59101 ctgtttctcg ccgttgcgac gatatttata ttcgatatcg tcgttgtcga ggttacggtc
    59161 gcccagcaca atagtgtgcg gaataccgat cagttccata tcagcaaaca tcacgcccgg
    59221 acgctctttg cggtcatcca gcagcacttc gataccttgt gcacgcagtt cgctgtacag
    59281 tttctcagca agctcttgta cgcggaagga tttgtgcatg ttcatcggca gaatcgccac
    59341 ctggaacggc gcgatagcgt caggccatac gatgccgcgt tcgtcgtagt tctgctcaat
    59401 cgccgcagct accacacgcg ttaccccgat accgtagcaa cccatcgtca ggatttggtt
    59461 acggccatct tcaccctgta cggaggcttt cagtgcttcg gagtacttgg tacccagctg
    59521 gaagatgtga ccaacttcga taccacgttt gatcagcagc gtaccctggc catccgggct
    59581 tggatcgcca gccaccacgt tacggatatc tgcaacttcc ggggtagcga catcgcgatc
    59641 ccagttgata ccgaagtagt gtttaccatc gatgttagca ccagcagcga aatcactcat
    59701 cgccgcaacg gtacggtcaa tcaccaccgg aatcggcatg tttaccggac ccagtgaacc
    59761 cggaccggct ttaaccacag cacgaatttc ttcttcggtc gcgaaagtca gcgggcttgc
    59821 aacctgcggc agtttttctg ctttaacttc gttcagctcg tgatcaccgc gcaccagcag
    59881 cgcaaccagc gggaagctac tgccttcaac cgctttaacc agcagagtct taaccgtttt
    59941 ctcaatcggc agattgaact gttcaaccag ttccgcgatg gttttcgcgt tcggcgtatc
    60001 aaccagcgtc atttcctggg tagcagcagc gcgcggttct ttcggcgcga tagcttctgc
    60061 cagttcaatg ttcgctgcat agtcagaggt gtcggagaag accacatcgt cttcaccgct
    60121 ctgcgccagc acctggaatt cgtgagaggc gctgccgccg atagaaccgg tgtcggcctg
    60181 tacggcgcgg aaatccagcc ccatgcggct gaagattttg ctgtaggccg catacattgc
    60241 atcgtaggtt tcctgcaggg attcctgaga agtatggaaa gagtaagcat ctttcatcag
    60301 gaattcgcgg gaacgcatga cgccgaaacg cggacgcact tcgtcgcgga acttggtctg
    60361 gatctgatag aagttcagcg gcagctgttt gtaagagcta agctcgttac gaatcaggtc
    60421 agtgataact tcttcatgag ttgggccgag tacgaacgga cgctcgccac ggtcaacaaa
    60481 acgcagcagt tccggaccgt actgttccca acgaccactc tcttgccaca aatcggctgg
    60541 ctgaaccacc ggcatcgaca cctcgatcgc accggcgttg ttcatctctt cacgcacgat
    60601 gttttcgact tttttcagaa cgcgcacgcc ggtcggcagc caggtatata acccggaggc
    60661 cagcttgcgg atcatcccgg cgcgcagcat cagctgatgg ctgatcacct cggcgtcggc
    60721 aggtgtctcc ttgagagtgg agagcaggta ttggctagta cgcatgttgt tacggttcca
    60781 gttggaaggt agaacaggct caaggcgagc ctgggacaaa aaaagtgatt tagtttacca
    60841 gtgcaaaaga aatgtcaaaa gagaagggcg tgaatttaac gcggttccag cgcaaagact
    60901 tcaaaacctg cgtcggtgac gcgccagcga acgttaaaat catgtagcca gacggcatag
    60961 gttttgcccg tttcctcacc tttacgatag gccgggcgcg ggtcctgcgc cagtacttcg
    61021 cggataaaca gcgttaactg cggataacgc ttctccagcg tcaaaagctg cttttcgacc
    61081 tctgcggtaa aactcaccgc catctctgca gctggcgcac tttgcgcata gctggcactg
    61141 gcatcgggaa gcgattcggc aaaggggaga tacggtttga tatccactac cggcgtacca
    61201 tcgaccagat ccagactgcc gagcttcaga atcacgctgt ctttatggca aacaacctct
    61261 ttcagctcta ccagcgacat gccaattggg ttagggcgga aagtagagcg tgttgcgaaa
    61321 acccccattc tggcgttacc gccgaggcgc ggtggacgca cagtcggacg ccagccgcct
    61381 tccatcgttt gatgaaagac gaaaaggatc cataaatggc tgaatgcttc caggccgcga
    61441 acggcgtcgg cctggttgta ggaagcaatg agatgcagtt cgccgttggc gctttttacc
    61501 agacctggct ggcgcggaac ggcgaatttt tctttatagg gcgagcgaat aacgcctatt
    61561 tgctcgaact ggaaactgct catttcgccg taatgttaag cgcagagccg atacatacag
    61621 cctgacgata gcagcccggc gtaccgctgg tgacttcgca gctatgcagt aatacagcat
    61681 tggctttcat tttagaggca ttgatttgca tctgcttacg tgcggttgga atgctcggcg
    61741 gagagtcctg attagaggcc tggcaagagt cgccactgac ttcaccgaga tcgcggaacg
    61801 gtttgccgac taattcttct gcattggtat aaattcggac cggcgtggcg cgcggcgctt
    61861 tcggttttgc aggctccgct ttcggctggg gtgcagtgct ttgaacgggt tcgacagggg
    61921 atctgcttaa catggaacag ccgcttagca tgagtgctac taaacagatc ggtaaagcac
    61981 gcatagtatt tcctcaatgt atgatcaaaa cgtcaatatt gaatcaggag cttgtaaaaa
    62041 tgacaagacg ggcaagcgcc cgtcctgaat gatattacaa attgtggaaa cagcctaaaa
    62101 attaccagcc tttaacagcg ccgccgttaa acactttgtt tgctgcttcg taaacttcat
    62161 cagactgata agcctggacg aatttcttca cgttctcggc gtctttgtta tcttcacgcg
    62221 ttacgatcag gtttacgtat ggggagtctt tatcttcgac aaagataccg tctttcgccg
    62281 gagtcaggcc aatctggctg gcataggtgg tattgataac tgccagagcg atttgcgcgt
    62341 cgtccagaga acgcggcagt tgcggtgctt ccagttcaac aattttcaga ttttttgggt
    62401 tctcaacaac atcaagaact gtcggcagca ggccaacgcc atctttcagt ttgatcaagc
    62461 ccactttttg cagcagcagc agtgaacgac caagattagt tggatcgttt ggcacggcaa
    62521 cctgcgaacc atcctgcagt tcatccagtg atttgatttt cttggagtaa ccagcaatcg
    62581 gataaacaaa agtgttgcct actgcgacca gtttgtagcc acgatctttc agttgctgat
    62641 caaggtacgg tttatgctgg aaggcgttgg cgtcgatatc gcctttgctc aatgcttcgt
    62701 ttggcagaac atagtcgttg aaggttacca gctcaacgtc caggccatat ttgtctttcg
    62761 caactttctg cgcgacttct gctacctgct gttcggcacc aacaatcacg ccgactttaa
    62821 tgtggtttgg atctttttca tcctgaccgc agcctaccag tgccagtgat ccaatcaggg
    62881 ctcccactgc cgcaaaggtt ttgaatttga acgccatacc ttattccttc ttcaattatt
    62941 tatgttgtgt tgaacgttac ttgcgagtga cagcccggac gatgcggtcg cctgcgaact
    63001 gaattaaata aaccagaatg accagcaata ccagtaccgt attcatcacc gtcgcgttgt
    63061 agccgatgta gccatactga tagccaatct gacctaaacc accggcaccg actgcaccac
    63121 ccatcgcgga ataaccgacc agggtaatca gggtgatagt tgccgcattc accagacccg
    63181 gcaacgcttc cggtaacagc actttacgga cgatctgcat tggcgtggca cccattgcgc
    63241 gggaagcttc aattaacccg gttgggatct ccagcagagc gttctcgacc atacgggcaa
    63301 taaacggtgc tgcaccaacg gttaacggaa caattgctgc ctgcaaacca atcgatgtac
    63361 cgacaataac gcgggtaaac ggaatcatcc ataccagcaa gataatgaac gggatggaac
    63421 ggaaaatgtt cacaatcgca gaaacggtac gatacagctt cgcgttagca ataatttgcc
    63481 ccggacgcgt gacataaagc agaacgccaa ccggcagacc aatcacaaac ccaaaaaatc
    63541 cggatacgaa ggtcattgcc agcgtttccc atacgccacg aaccagcagc cacatcatcg
    63601 gctcagacat aacccagtac ctctactttt acatggtgtt cctgcagcca ggcaatggcg
    63661 gcttgcgtat cttgttgtgt gccgtgcatt tcagtcagca tgatgccgaa tttaacgcca
    63721 ccggcgtagt ccatctgcgc gctaataatg ttgttgttga cgttgaaacg acgcgcggtt
    63781 tcagaaagca gtggggcatc gaccgattga ccggtaaact ccagacgcag catcggcacg
    63841 cagtcagtaa atggctccgt ttgcagacgt tcctggtaat cttccgggat atccagatgc
    63901 agggtcgact gaataaactt ctgcgccagc ggcgttttcg gatgcgagaa cacttcactt
    63961 accgtgtcct gctcgatcag ttcgccattg ctgatgaccg ccacgcaatc acaaatacgc
    64021 ttcacaacgt ccatttcgtg ggtgatcaac agaatcgtca aacccagacg gcggttaatg
    64081 tctttcagca gttcgagaat agaacgtgtc gttgccgggt ccagcgcgct ggtggcttca
    64141 tcacacagca atactttggg attgctggct aatgcacggg caatcgccac acgttgtttc
    64201 tgcccaccgg aaagattcga cgggtagcta tcatgcttat cgccaagacc aaccaatgac
    64261 agcaattccg tcacgcgacg tttgatctcg tctttcggtg tgttgtccag ctccagcggc
    64321 agagccacgt tgccaaaaac agtacgcgaa gagagcaggt taaaatgctg gaaaatcata
    64381 ccaatctggc ggcgagcttt ggtcaactcg gattctgaca gcgtggtcaa ttcctggcca
    64441 tcgaccagca cgctaccctc ggttgggcgc tccagcaggt ttacacaacg tataagcgta
    64501 ctcttacccg cgcctgaggc accgataacg ccataaattt gtccagctgg cacatgcagg
    64561 ctgacgttgt tcaacgcctg gatggtgcgg gtgccctggt ggaacacttt ggtgatattc
    64621 gaaagtttta tcattgatta tttattatcg tcattaagtt agtcgtagca tctcgaatgc
    64681 ctgaaacggg caacgccgtc aatgaaatgg atgttaaggc atccagacgt ctaaatcaat
    64741 caggtttatg cgaagagcac tttcttgcag gtcgaaacat gcgatactag cgtcccatgc
    64801 cttattaagg agctataaaa ggtggcgaag agcgtacccg caatttttct tgaccgtgat
    64861 ggcaccatta atgtcgatca cggctatgtc catgagatcg acaactttga atttatcgac
    64921 ggtgttattg acgccatgcg cgagctaaaa aaaatgggct ttgcgctggt ggtagtaacc
    64981 aaccagtctg gcattgctcg cggtaaattt accgaagcac agtttgaaac gctgaccgag
    65041 tggatggact ggtcgctggc ggaccgagat gtcgatctgg atggtatcta ttattgcccg
    65101 catcatccgc aggggagtgt tgaagagttt cgccaggtct gcgattgccg caaaccacat
    65161 ccggggatgc ttttgtcagc acgcgattat ttgcatattg atatggccgc ttcttatatg
    65221 gtgggcgata aattagaaga tatgcaggca gcggttgcgg cgaacgtggg aacaaaagtg
    65281 ctggtgcgta cgggtaaacc tattacacct gaagcagaaa acgcggcaga ttgggtgtta
    65341 aatagcctgg cagacctgcc gcaagcgata aaaaagcagc aaaaaccggc gcaatgatta
    65401 aaagatgaac ggttgaaata aaaatgcatt tttccgcttg tcttcctgag ccgactccct
    65461 ataatgcgcc tccatcgaca cggcggatgt gaatcacttc acacaaacag ccggttcggt
    65521 tgaaga
//
LOCUS       DADMEQ010000009        65143 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.009, whole genome shotgun sequence.
ACCESSION   DADMEQ010000009 DADMEQ010000000
VERSION     DADMEQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65143
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.009"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="JNZ05_000587"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="JNZ05_000587"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAY8563935.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="JNZ05_000588"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="JNZ05_000588"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HAY8563936.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="JNZ05_000589"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="JNZ05_000589"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HAY8563937.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="JNZ05_000590"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="JNZ05_000590"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HAY8563938.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPTRGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="JNZ05_000591"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="JNZ05_000591"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HAY8563939.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLHQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /gene="cptA"
                     /locus_tag="JNZ05_000592"
     CDS             7826..9559
                     /gene="cptA"
                     /locus_tag="JNZ05_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HAY8563940.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="JNZ05_000593"
     CDS             9708..10559
                     /locus_tag="JNZ05_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8563941.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLRGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="JNZ05_000594"
     CDS             complement(10546..10887)
                     /locus_tag="JNZ05_000594"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY8563942.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="JNZ05_000595"
     CDS             complement(10889..11767)
                     /locus_tag="JNZ05_000595"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HAY8563943.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCSWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="JNZ05_000596"
     CDS             complement(11733..14030)
                     /locus_tag="JNZ05_000596"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY8563944.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQGRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGHDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="JNZ05_000597"
     CDS             complement(14081..14401)
                     /locus_tag="JNZ05_000597"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY8563945.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="JNZ05_000598"
     CDS             complement(14416..15495)
                     /locus_tag="JNZ05_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HAY8563946.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..18305
                     /gene="ptsP"
                     /locus_tag="JNZ05_000599"
     CDS             15804..18305
                     /gene="ptsP"
                     /locus_tag="JNZ05_000599"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY8563947.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="JNZ05_000600"
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="JNZ05_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY8563948.1"
                     /translation="MELYLDTANVTEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAEGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQTLLEMHAPES
                     MVLAASFKTPCQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            18990..20082
                     /gene="gldA"
                     /locus_tag="JNZ05_000601"
                     /pseudo
     CDS             18990..20082
                     /gene="gldA"
                     /locus_tag="JNZ05_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20357..20974
                     /locus_tag="JNZ05_000602"
     CDS             20357..20974
                     /locus_tag="JNZ05_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HAY8563949.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQECGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21001..21939)
                     /gene="yijE"
                     /locus_tag="JNZ05_000603"
     CDS             complement(21001..21939)
                     /gene="yijE"
                     /locus_tag="JNZ05_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HAY8563950.1"
                     /translation="MSTGKLHTGSLMSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVT
                     SYIGAFDFTALRCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWAL
                     VSGGAGKVAILSYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFS
                     SMKSAMLAILSGVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQ
                     REIDWQPTVFWALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWL
                     LGENPGAVEGSGIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22000..24180)
                     /gene="katG"
                     /locus_tag="JNZ05_000604"
     CDS             complement(22000..24180)
                     /gene="katG"
                     /locus_tag="JNZ05_000604"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HAY8563951.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGVNGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24509..25399)
                     /gene="metF"
                     /locus_tag="JNZ05_000605"
     CDS             complement(24509..25399)
                     /gene="metF"
                     /locus_tag="JNZ05_000605"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HAY8563952.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGICHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25748..28180)
                     /locus_tag="JNZ05_000606"
     CDS             complement(25748..28180)
                     /locus_tag="JNZ05_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HAY8563953.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28183..29343)
                     /gene="metB"
                     /locus_tag="JNZ05_000607"
     CDS             complement(28183..29343)
                     /gene="metB"
                     /locus_tag="JNZ05_000607"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAY8563954.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29620..29937
                     /gene="metJ"
                     /locus_tag="JNZ05_000608"
     CDS             29620..29937
                     /gene="metJ"
                     /locus_tag="JNZ05_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HAY8563955.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30121..30729
                     /locus_tag="JNZ05_000609"
                     /pseudo
     CDS             30121..30729
                     /locus_tag="JNZ05_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
     gene            complement(30790..31002)
                     /gene="rpmE"
                     /locus_tag="JNZ05_000610"
     CDS             complement(30790..31002)
                     /gene="rpmE"
                     /locus_tag="JNZ05_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAY8563956.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31205..33403
                     /gene="priA"
                     /locus_tag="JNZ05_000611"
     CDS             31205..33403
                     /gene="priA"
                     /locus_tag="JNZ05_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAY8563957.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33559..34584
                     /gene="cytR"
                     /locus_tag="JNZ05_000612"
     CDS             33559..34584
                     /gene="cytR"
                     /locus_tag="JNZ05_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HAY8563958.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34676..35635
                     /gene="ftsN"
                     /locus_tag="JNZ05_000613"
     CDS             34676..35635
                     /gene="ftsN"
                     /locus_tag="JNZ05_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HAY8563959.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35728..36258
                     /gene="hslV"
                     /locus_tag="JNZ05_000614"
     CDS             35728..36258
                     /gene="hslV"
                     /locus_tag="JNZ05_000614"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HAY8563960.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36268..37599
                     /gene="hslU"
                     /locus_tag="JNZ05_000615"
     CDS             36268..37599
                     /gene="hslU"
                     /locus_tag="JNZ05_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HAY8563961.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37666..38592
                     /gene="menA"
                     /locus_tag="JNZ05_000616"
     CDS             37666..38592
                     /gene="menA"
                     /locus_tag="JNZ05_000616"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAY8563962.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38685..39170
                     /gene="rraA"
                     /locus_tag="JNZ05_000617"
     CDS             38685..39170
                     /gene="rraA"
                     /locus_tag="JNZ05_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HAY8563963.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39255..39500)
                     /gene="zapB"
                     /locus_tag="JNZ05_000618"
     CDS             complement(39255..39500)
                     /gene="zapB"
                     /locus_tag="JNZ05_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HAY8563964.1"
                     /translation="MTMSLEVFDKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39926..40771
                     /gene="glpF"
                     /locus_tag="JNZ05_000619"
                     /pseudo
     CDS             39926..40771
                     /gene="glpF"
                     /locus_tag="JNZ05_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40811..42301
                     /gene="glpK"
                     /locus_tag="JNZ05_000620"
     CDS             40811..42301
                     /gene="glpK"
                     /locus_tag="JNZ05_000620"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAY8563965.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYISSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEENYALEGAVFMAGTSIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLSAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42531..43541
                     /gene="glpX"
                     /locus_tag="JNZ05_000621"
     CDS             42531..43541
                     /gene="glpX"
                     /locus_tag="JNZ05_000621"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY8563966.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43638..44384
                     /gene="fpr"
                     /locus_tag="JNZ05_000622"
     CDS             43638..44384
                     /gene="fpr"
                     /locus_tag="JNZ05_000622"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HAY8563967.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44389..44817)
                     /gene="uspD"
                     /locus_tag="JNZ05_000623"
     CDS             complement(44389..44817)
                     /gene="uspD"
                     /locus_tag="JNZ05_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HAY8563968.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44844..45143)
                     /locus_tag="JNZ05_000624"
     CDS             complement(44844..45143)
                     /locus_tag="JNZ05_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HAY8563969.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAAFSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45355..45795)
                     /locus_tag="JNZ05_000625"
                     /pseudo
     CDS             complement(45355..45795)
                     /locus_tag="JNZ05_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45896..46495
                     /locus_tag="JNZ05_000626"
     CDS             45896..46495
                     /locus_tag="JNZ05_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HAY8563970.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46603..47370
                     /gene="tpiA"
                     /locus_tag="JNZ05_000627"
     CDS             46603..47370
                     /gene="tpiA"
                     /locus_tag="JNZ05_000627"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAY8563971.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47425..48180)
                     /gene="cdh"
                     /locus_tag="JNZ05_000628"
     CDS             complement(47425..48180)
                     /gene="cdh"
                     /locus_tag="JNZ05_000628"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HAY8563972.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMSTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48287..49276)
                     /locus_tag="JNZ05_000629"
     CDS             complement(48287..49276)
                     /locus_tag="JNZ05_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8563973.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49596..50558)
                     /gene="pfkA"
                     /locus_tag="JNZ05_000630"
     CDS             complement(49596..50558)
                     /gene="pfkA"
                     /locus_tag="JNZ05_000630"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAY8563974.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50739..51641)
                     /gene="fieF"
                     /locus_tag="JNZ05_000631"
     CDS             complement(50739..51641)
                     /gene="fieF"
                     /locus_tag="JNZ05_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HAY8563975.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51789..52289)
                     /gene="cpxP"
                     /locus_tag="JNZ05_000632"
     CDS             complement(51789..52289)
                     /gene="cpxP"
                     /locus_tag="JNZ05_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HAY8563976.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52439..53137
                     /gene="cpxR"
                     /locus_tag="JNZ05_000633"
     CDS             52439..53137
                     /gene="cpxR"
                     /locus_tag="JNZ05_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HAY8563977.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53134..54507
                     /gene="cpxA"
                     /locus_tag="JNZ05_000634"
     CDS             53134..54507
                     /gene="cpxA"
                     /locus_tag="JNZ05_000634"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HAY8563978.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTKLLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54613..55287)
                     /gene="yiiM"
                     /locus_tag="JNZ05_000635"
     CDS             complement(54613..55287)
                     /gene="yiiM"
                     /locus_tag="JNZ05_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HAY8563979.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(55436..56419)
                     /gene="kdgT"
                     /locus_tag="JNZ05_000636"
     CDS             complement(55436..56419)
                     /gene="kdgT"
                     /locus_tag="JNZ05_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HAY8563980.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAIIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(56679..57299)
                     /gene="sodA"
                     /locus_tag="JNZ05_000637"
     CDS             complement(56679..57299)
                     /gene="sodA"
                     /locus_tag="JNZ05_000637"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HAY8563981.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            57584..58618
                     /gene="rhaT"
                     /locus_tag="JNZ05_000638"
                     /pseudo
     CDS             57584..58618
                     /gene="rhaT"
                     /locus_tag="JNZ05_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(58615..59552)
                     /gene="rhaR"
                     /locus_tag="JNZ05_000639"
                     /pseudo
     CDS             complement(58615..59552)
                     /gene="rhaR"
                     /locus_tag="JNZ05_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709710.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(59536..60372)
                     /gene="rhaS"
                     /locus_tag="JNZ05_000640"
                     /pseudo
     CDS             complement(59536..60372)
                     /gene="rhaS"
                     /locus_tag="JNZ05_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
     gene            60660..62129
                     /gene="rhaB"
                     /locus_tag="JNZ05_000641"
     CDS             60660..62129
                     /gene="rhaB"
                     /locus_tag="JNZ05_000641"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAY8563982.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELITLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            62126..63385
                     /gene="rhaA"
                     /locus_tag="JNZ05_000642"
     CDS             62126..63385
                     /gene="rhaA"
                     /locus_tag="JNZ05_000642"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAY8563983.1"
                     /translation="MTTQLEQAWELAKQRFAAVRIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            63627..64451
                     /gene="rhaD"
                     /locus_tag="JNZ05_000643"
     CDS             63627..64451
                     /gene="rhaD"
                     /locus_tag="JNZ05_000643"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAY8563984.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLAVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            64461..64775
                     /gene="rhaM"
                     /locus_tag="JNZ05_000644"
     CDS             64461..64775
                     /gene="rhaM"
                     /locus_tag="JNZ05_000644"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAY8563985.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            65076..>65143
                     /locus_tag="JNZ05_000645"
     CDS             65076..>65143
                     /locus_tag="JNZ05_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="with FrvBRX is part of the
                     phosphoenolpyruvate-dependent sugar phosphotransferase
                     system involved in the transport and phosphorylation of
                     sugars; possibly responsible for the sugar specificity of
                     the system; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAY8563986.1"
                     /translation="MAALTASCIDLNIQGNGAYSVL"
BASE COUNT        15887 a        17549 c        16663 g        15044 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccaacacg cggagttaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acacaaccag ctgatgcgtc
     5521 gcctgcacca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctaggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttcct ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atagcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgcgcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac attttgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgagcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc agcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cgtgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggccctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccaatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggagtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt cgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctggctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gccgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcacag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaag agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgtgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcagtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt taggttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctctaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctcac
    19741 gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca cggtgaaaaa
    19801 gtggcatttg gtacgctgac gcagctggta ctggaaaacg cgccggtgga ggaaatcgaa
    19861 accgtagctg cacttagcca tgcggtaggt ttgccaataa ctctcgctca actggatatt
    19921 aaagaagatg tcccggcgaa aatgcgcatt gttgcaaaag catcttgtgc agaaggtgaa
    19981 accatccaca acatgcctgg cggcgcgacg ccagatcagg tttacgccgc actgctggta
    20041 gctgaccagt acggtcagcg tttcctgcaa gagtgggaat aacctactcc aaactcccgg
    20101 cttgtccggg agtttgaacg caaaattgcc tgatgcgcta cgcttatcag gcctacgcaa
    20161 tctctgcaat atattgaatt tgcgtgcttt tgtaggccgg ataaggcgtt cactccgcat
    20221 ccggcactct gtgccagtaa tctgaaactc ccacgttgtt caggagtttt ttatccgctt
    20281 ctggacattt tctccacaga acaatcgtta gcccggaagg tcgaatctgt gactcttatt
    20341 ttcacggacg aaccagatga aagcttcact ggcgctgctc agcctgctaa cggcatttac
    20401 cagccactcc ttaaaatctc ccgccgttcc accaaccgtg gtacaaatcc aggccaatac
    20461 caaccttgcc atcgcagacg gtgccagaca gcaaattggc agcacgctat tttacgatcc
    20521 ggcgtatatg cagcttacct atcctggcgg tgatgttccg caagaatgcg gtgtatgttc
    20581 cgatgtggtg atccgcgcat tgcgcagcca gaaggtcgat ttgcagaaac tggttcacga
    20641 agatatggcg aagaattttg ctgagtaccc gcaaaaatgg aagttaaagc gcccggacag
    20701 caacatcgat caccgtcggg tgcctaatct ggaaacctgg tttagccgcc acaacaaaac
    20761 acgccccatc agtaaaaatg ccggtgacta tcaagcgggc gatattgtct cctggcgatt
    20821 agataacggg ctggcgcata ttggggtggc gtcagatggc ttcgcccgcg acggtacgcc
    20881 gctggtgata cataacattg gcgcaggcgc gcaggaagaa gatgtgctgt ttagctggcg
    20941 gatggtgggg cattatcgtt attttgttaa ataagagatc gccccacatg aagaaaaaat
    21001 tcagatgctt tttacactga cagcttcttt tttcttacgg ctcaccagcg ccagtgccag
    21061 cacaatcagc acaataccgc taccttcaac ggcccccgga ttctcaccga gcagccacca
    21121 ggaaaagagt acgccacaaa cgggaacggc cagtgtgctt aagctggcaa tactggcagg
    21181 caagtttttc agcacaaaca gccataagct ccacgctagc gccgtcgcca gaatcgcact
    21241 gtaggccagc gcccagaaca cggtgggctg ccagtcaatt tcacgttgcg gcaccagtaa
    21301 agcgaccaca ctcatcacca gcgccgcata cagcatctgc caggatgtta acgataataa
    21361 atccacgcgc ggatgacggg cgtataggcg tttagcaaca atcgcgctcg ccccccaact
    21421 aacaccggag aggattgcca gcatggcact tttcatcgaa gagaaatcga gttgccacgg
    21481 ctgcaacacc aaaaataagc cgaaagcggc aatcagaatc gcgaaatatt gcccacgtcg
    21541 caggcgttca ccgagaaaca acgcggcgaa aatcaccacc cagaacggca tggtatagct
    21601 caggatcgcc accttccccg cacctccgct gaccaacgcc cactgcgcca gaccaaccat
    21661 cccgcaggtt tgtaacaggg caatggctaa ggtgtattta aacggcgtcg ggcgcattcc
    21721 gcgaccacgt aataaaagga cgatgaataa aacgagagcg ccgaaaatgc agcgtaaggc
    21781 ggtaaagtcg aaggcaccga tgtaactggt gacttgcttc atgaaaatcc agctataact
    21841 ccagataagt gtgagcacaa ccaggccact gattgccagt gggttgctct ttcctgcggc
    21901 agacatgaga gatccagtgt gtagttttcc tgtggacact atacgccggt aaagaaactt
    21961 cagcgactgc ctgaaagaca gtcgctgaag aaggtcagat tacagcaggt cgaaacggtc
    22021 gaggttcatc actttcaccc atgccgctac gaagtcttta acaaacttct cgtgggcatc
    22081 gctgctggcg taaacttccg ctactgcacg caggacagag ttagaaccaa ataccagatc
    22141 cgcacggctg gctgtgtatt tcacttcgcc ggtttcgcgg tcacgtcctt cgaacagctc
    22201 tttcgattcg tcagtcgctt tccactcgta acgcatatcc agcaagttca cgaagaagtc
    22261 attgctcaat acgccaacgc ggtcagtgaa gacgccgttt ttgctgccat cgaagttggc
    22321 acccagtaca cgcatcccgc ccaccagcgc agtcatttcc ggcgcggtca gcgtcagttg
    22381 ctgtgctttg tcgatcagca gtgactcggt ggtggaaacg tccagacgag cgcgatagtt
    22441 acggaaaccg tcagcaattg gctccagcag ctcaaacatc tcaatgtcag tctgatcctg
    22501 acgcgcatca acgcgacccg gcgcaaacgg tacatgaatg ctcaaacctg cggcgcttgc
    22561 ggctttctca acaccaacca caccagccag cacgatgata tccgccagcg aggctttacc
    22621 agactctttc tggattttct ccagaacagg cagagcacga acggctgcgg cgttcacatc
    22681 ccagtcgcgc tgcggcatta atgccagacg cgcaccgttg acaccaccgc gtttgtcgcc
    22741 accacggaag gtagaagcag atgcccaggc caccgatacc agctcactaa cagacagacc
    22801 agaatccgca atcgcgaatt tcagatcgat aatgtcctgc tcggtcgggt tgtagatcgg
    22861 ctgcggcagc ggatcttgcc agatcagatc ttctttcggc acttccggcc cgatgtagcg
    22921 agatttcggc cccatatccc tgtgcgtcag tttgaaccag gcacgggcaa aggcttcgtt
    22981 gaacgcctgc ggatcgttga ggaaacgacg agagatcttc tcgaactcag gatcaaaacg
    23041 cagcgtcagg tcggtcacca gcattgtcgg tttacgtttc ttcgacggat cgaacggatc
    23101 cgggataatt tccggtgcgt ctaccgcttc gaactggatt gctccagccg gactgcgggt
    23161 ctgtacccac tcatacttga acagattctc gaagaaatag ttgctccact gggtcggcgt
    23221 ctgggtccag actacttcca gaccagaggt aatggcatct gcgccaacgc cgctgccgta
    23281 agtgctcgcc cagcctaaac cttgttcttc aatcggtgca gcttctggat caggacctac
    23341 gtttgatgtc ggaccggcac cgtgggtttt acccagcgta tgaccacccg caatcagcgc
    23401 cacggtttct tcgtcgttca tgcccatgtt gccgaaggtc gcgcggatag ctgctgccgc
    23461 agaaagcggt tcgccgctgt gatccgggcc ttccgggtta acgtaaatca ggcccatctc
    23521 ggttgcaccc agcggtgctt tcgccagcgc ttccggatga cggtgagtca gccaggcttt
    23581 ttcatcaccc cagttaacat ccagatccgg ttcccagacg tcttcacgac cggcaccaaa
    23641 accgaaggta cggaagccgg agttttccag cgccacgtta cctgcgagga taaacaggtc
    23701 ggcccaggag attttctgac catatttctg tttgattggc cacaacaggc gacgcgcttt
    23761 atcgaggctt acgttatccg gccaggagtt cagcggtgca aaacgttgct gaccacgacc
    23821 cgcgccgccg cgtccatcga ttgaacggta agtacctgcg ccgtgccaag ccatacgaat
    23881 aaacagaccg gcgtagctgc cccagtcggc tggccaccac ggctgagatt ctgtcagcag
    23941 ggctttcaga tcttttttca ggccgtagta atctaatttg ctgaattctt tgcggtagtc
    24001 aaagtcctca cccagcgggt tagaacgatt agaatgttgg tttaacaggt caacacggag
    24061 ttgatttggc caccagtcgc gagtggttgt gcccgccccc gcgctctggt cgtgaccgcc
    24121 ctgatggaac gggcatttgc cagtggctgt ggtgttatgg atatcgtctg acgtgctcat
    24181 caatgtgctc ccctctacag tgttaccgtt acgatacaca gcgttagaga gaagttataa
    24241 ttgaattaat ccacggatgc gatagttgag atcttacata ttgttggtta aagagatgta
    24301 gatcaaattg atcgtaatta agcttttttg attatgtccg aattcggaca aatgctttat
    24361 aaaaaagggt tttagcttca tatccttcaa gtaatgaggg agaaaacaag gctgacaacc
    24421 ttattttccc gccctcattt cgaggcagca ttttgtgctc tgtttaaaat ttgtgatcac
    24481 tgtgtgattt tcacaaaagc cacactattt ataaaccagg tcgaacaccc agcgtatggc
    24541 aaatcgcgta actcatttca gcgcggttaa gcgtatagaa gtggaaatct ttcactcctt
    24601 cacggcttaa aatcttcacc atatccatgg caatattcgc gcccaccagt ttgcgggttt
    24661 cggcatcgtc atccagcccg tcaaacattt gcgccatcca cgccggaata cgcacgttgg
    24721 tcatgtcggc aaatttcttc gcctgtttaa agttagatac cggcaaaatg cccgggatga
    24781 tttccacatc aatgcccgcc gatacacagc ggtcacgaaa acgcaggtag ctttcgacat
    24841 cgaagaagaa ctgagtaatc gcgcggttgg ctccggcatc cactttgcgt ttcagattaa
    24901 gcaaatccgc ctgagcgctt tttgcttccg ggtgaacttc cggatacgcc gccacggata
    24961 tatcgaaatc tgccacttct tttaacagcg tcaccaggtc agaagcatac atttctggct
    25021 taccacttcc cggcggcaga tcgccacgca gtgccacgat atgacaaata ccgttattcc
    25081 agtagtcgcg tgcaatggtg cgcagctcgt cgggcgtcgc atcaatgcaa gtaagatgcg
    25141 gtgccgcttc cagaccagtg cgatctttaa tgcctttaat aatgctgtgc gtacggtcgc
    25201 gctcgccgga gttcgcgcca taggtcaccg atacaaactt cggtttcagg ctgctaaggc
    25261 gatcgatgga gttccacagg gtctgctcca tttcactggt acgcggcggg aaaaactcga
    25321 acgaaacgtt aatctgcccc tggacttctg ccaggctctg attcagggca tcccgctggc
    25381 tggcgtgaaa aaagctcata ccttacctca tcaatcgctt gtagttatat gttgtgttgt
    25441 gcacatctat ccatttagac gtccagatgt aaagatgaag gaaaagccga agggcgtcaa
    25501 ccgaaaaatg accaatgatg gtgaggaaaa atcagggaag atgaaaaaac ttcaggatgg
    25561 taagaaaaag aaaatccgat atgcaaagca aaccggactg caaatataaa ctaaccatgt
    25621 acttattgct tcttgccgga ttcgtcgtaa acgcttcatc cggcctaatt ctatgataat
    25681 tcaatatatt tcattagaaa ccatagaccc gaaaacatga gtaccgggca ttattaaatt
    25741 tctgaaatta caacaactgt gccagccggt tgatatccga ctgaatcgcc ccggcggtga
    25801 cgtcgcgccc agcgccaggc ccacggatca ccagcggatt atcgcggtac cagcggcttt
    25861 cgatggcaaa gacgttatcg catggcagca gtgatgccag cggatggtct tcacgcaccg
    25921 cctccacgcc cacacgcgct ttgccgttgg catcgaaacg cgccacgtag cgcagcacca
    25981 gccccatttc gcgggccgct tccagccgtt gcaccatctg ctcgttaagt tcatcgccat
    26041 tttcaaagaa atggtcgatg ctgccacctt cgcaatgagc aggcaccagc gattccacac
    26101 gtacctggtc cggttcaatg ttgtaacccg cttcacgcgc cagaatcacc agcttgcgca
    26161 ttacgtcttt gccggagagg tcgtcacgcg gatcaggctc ggttaagccc tgctgccacg
    26221 cctgatccac cagctcggta aacggcacgc taccgtcgaa ttgcaggaac agccaggaga
    26281 gcgtgccgga gaagatccca ctgatcgaca aaatggtatc gccgctgtcg atcagatcgc
    26341 gcacggtatg gttgatcggc aagcccgcac caacggtggc gttgtatagc cagtgacgcc
    26401 cggttttttc gaaggcgtcg tggatctggc gatatttatt gctgtcgctt gctcccgcca
    26461 gtttgttggc actgatgacg tggaaaccgt ggctggcgaa atcgagatac tgatcagcaa
    26521 gctgctggct ggcggtaacg tccaacacca ctaaatcatc atacggatgg gcgcgcatcc
    26581 acaggaaaag cgactcttca tcctgctcga ccgcttcatc gttgaagaag gctaacgcgc
    26641 ggctggcgtc cagcccgtca taactcaaca gactgcggcg gctgtccacc acgcctgcca
    26701 gcacaaattc aaagccagta cgcgccgaaa gcgtgctctg ctcgcgggcg aacagttcca
    26761 gccagcgaga gccgatattg cctttgccga acaataccag gccgatgcgt ttttctgcgc
    26821 ggaagacgga ctgatgcagc ccctgaatca ggctttcggt cgggccggtg cgcagtactg
    26881 ccaccagact gatgccgtca tcggactgcc acgtaaactc gaccggctgg cctttcagtt
    26941 gctgccagaa gcggtggcaa tgcagcgggt tacgggtgac acccgccccg accatcgcca
    27001 ccagcgccag cccctgacgc aggcgcagtt ctccaggtaa tcccgcttcg tcgaggattt
    27061 tcagcgcact gtcggccact tctgaggtgt agcaaaattg cagcaactgg cgatcgttat
    27121 gcacgccaac cgccagtgga cgcacctggg cacgtttcag gatctggtcg atctctttat
    27181 gcgccagttt gaaatcctga ctggcgggca cctgaaactc aatcaaacag acatcatcgt
    27241 ggctggtgac aatacgcgca ccagtaccgg aggccagcac gcgttcaatg cgcgtggaac
    27301 cttgatccgg cgtgtagcta cagcgcagct gcaggtcgat ttcgctgcca gaaactggct
    27361 gtaaagtacg ggcgtgaaga acgggagccg ccagacgcgc cagttcgctg gcctcatcca
    27421 gacgcagcaa cggcagcagg caggcatctt tcactttacg cgggtcggca ctgtataccc
    27481 cggcgacgtc gctccagatg gttacacgag aaacacccgc cagcgcaccg atttgtgtcg
    27541 cggaatagtc ggaaccgtta cgccccagca acaccgtttc accggcgttg ttacggctga
    27601 taaacccggt caccaccaga cgtttgctcg gatgttgtac cagtagctgt tgcagcaacg
    27661 ggtaagaaag cccttcatca acctgcggtt gcgcagcgcg ttcagcgcgt aaaaactcac
    27721 gggcatcaag ccaggccgct ggcagccctt gttgattaag taccgcagac atcaggcgcg
    27781 ccgaccacac ttcaccgtgg cccaccactt ccgcatacac cgcatcgtta ataccactgt
    27841 cgagcaacgc cgccaggcgc tcaagatcgc tgacaaaagc gctaatgagg ctatcggctt
    27901 cttcagcggg tagcagaccg ctaatcagat cgcactgata gcgacgcagc gtttgttgaa
    27961 cctgatgcgc agagagacga tcggtctggc ttagtttcaa ccagttaatc aactggttag
    28021 tggtgctacc ggcggcggaa accaccatca tatcgtcagg ctgagagaac tccgccataa
    28081 tgcccgcgac acgcaaataa cacttcacat cagccagact actgccacca aatttatgca
    28141 gctgacgacc tttcgcccct gcctgcgcaa tcacactcat ttttacccct tgtttgcagc
    28201 ccggaagcca ttttccaggt cggcaattaa atcttcgcca tcttcaatac cggtggagat
    28261 acgcagcagc gtctcggaga tcccggcggc agcacgcgct tctggtgcca tgcctgcatg
    28321 tgtcatggtt gcggcgtgag agattaaact ttccactccc cctaatgatt ccgccagcgt
    28381 aaacaacgac agcccgccca ggaaacgaca cagcgtctgc tcatcgccat ccagttcaaa
    28441 actcaacatt gcgccaaagc ctttttgctg acgcgcggca atttcatgcc cctgattttc
    28501 cggcagcgac gggtgataca gttttttcac caacggctgg gtttgcagat atttcacaat
    28561 cgcctgcgcg ttgcgctgcg ccagctccat acgcggcacc agcgttcgca acccgcgtag
    28621 cagcagatag ctgtcaaacg cgccgcccgt cacgccaata ttgtttgccc accaggcaag
    28681 ttcagtgaca acgtccgggt ctttagcaat caccacgcca gccaccacgt cagagtgacc
    28741 gttcagatat ttcgtacatg aatgcaacac cagatcggca cccaatgcca gcggattttg
    28801 taatgccggg cttaagaagg tgttatccac cacgctcacc gccccgactt cccttgccag
    28861 atggcagatt ttcgcaatat ccacgacgcg taacaatgga ttacttgggc tttctaccag
    28921 taccagtttg ggtttttctg ccagcgctgc ccgtaatgcc tgttcatcgc cttgatcaac
    28981 aaacaacacg cgatagcaac cgcgtttcgc cagactgtcg aacaggcgat agctaccgcc
    29041 gtagcagtcg tgcggcgcaa ccagcagatc gccaggtttc aaaaagacgg tcgttaccag
    29101 gtgaatcgcg gacatgccgg tattagtaag tactgcgcca gcaccgcctt ccagttctgc
    29161 cagcgcacgc tgaaccacat cgcgcgttgg gttgccgcga cgcgagtaat catgcgcgcg
    29221 cggttcatta aatccggtaa agttataggt gctggaaaga tggatgggag ggacaacgca
    29281 accatactgt tcgtcgtcat ttaacccgct acgcactgcg atggtggcct gtttacgcgt
    29341 catgtgatga agtttcctgg gctttgtcgg tgaaatgtca ggcaccagag taaacattgt
    29401 gttaatggac gtcaatacat ctggacatct aaacttcttt gcgtatagat tgagcaaatc
    29461 ccaaatagcc gttaaaatta tatgcattat cacgccgaca ggtgcattac acgatgtcac
    29521 ggtaacgcct gtacggtaaa ctatgcgggt ttacggtcag taccaacata aattgtgggg
    29581 tctggtctca atttattgac gaagaggatt aagtatctca tggctgaatg gagcggcgaa
    29641 tatatcagcc catacgctga gcacggcaag aagagtgaac aagtcaaaaa gattacggtt
    29701 tccattcctc ttaaggtgtt aaaaatcctc accgatgaac gcacgcgtcg tcaggtgaac
    29761 aacctgcgtc acgctaccaa cagcgagctg ctgtgcgaag cgtttctgca tgcctttacc
    29821 gggcaacctt tgccggatga tgccgatctg cgtaaagagc gcagcgacga aatcccggaa
    29881 gcggcaaaag agatcatgcg tgagatgggg attaacccgg agacgtggga atactaaagc
    29941 ggaaaagagc ggcgcggagt ggaatcgcct gatgcactac gcttatcagg cctacgtcat
    30001 attgcaattt attgaatttg cacgaacttg taggccggat aaggcgttca cgccgcatcc
    30061 ggcataaaca acgagcacgt tgtctgcgac ccaccgcttt ttatacatgg acgtttaact
    30121 atgaaaaaca ggctgctgat cctcagcctg ctggtttctg tacctgcctt tgcctagcag
    30181 ccgcaaactg gcgacatcat ctttcaaatc tctcgctcat cgcaaagtaa agcgatccaa
    30241 ctggcaaccc attccgatta cagccacacc ggtatgctgg tgatgcgtaa caaaaagccc
    30301 tacgtttttg aagcggtcgg cccggtgaaa tacaccccgc tcaagcagtg gattgcccat
    30361 ggtgaaaagg gcaactacgt tgttcgccgc gttgaaggcg gactgagtgt tgaacaacag
    30421 caaaaactga cgcaaacggc aaaacgttat ctcggtaaac cgtatgattt cagcttctca
    30481 tggagcgacg atcgccagta ctgttcggaa gtggtgtgga aggtttacca gaacgcgctg
    30541 ggaatgcgcg tgggcgaaca gcagaagttg aaagagtttg atctcagcaa cccgctggtc
    30601 caggcgaagc tcaaagaacg ctacggtaaa aatattccgc ttgaggaaac ggtagtttca
    30661 ccacaagccg ttttcgacgc gccacaactc accacggttg ccaaagagtg gccgctgttt
    30721 tcgtggtgat acgaccaaaa ttcaggcaca aaaaaagcgc cgtgcggcgc ttttttcgga
    30781 aatccggtct tatttgctgc ccgggatgtt gaaacgcttg ttgaagcggt caacacggcc
    30841 accggtagca acatcacgct gtttgccagt gaagaacggg tggcacttgc tgcacacgtc
    30901 gaggttcagg tcatgaccaa cggtggagcg gattttcatt acgttaccgc aagagcagct
    30961 agcagtaatt tcttcgtatt tcgggtgaat atcttttttc atgggaaaac ctcggtttaa
    31021 ggccgcgtcg ctcttccagc cctaacgcca gacaccacgc gatgttaaaa gtatagcttc
    31081 aatacgatca tttcgtacga aggcgcgaaa tcatacagaa attaaccagc gtatgcaaac
    31141 tgatccgcac tcttctacgg caatgtgtat actaacccac cgaatttcaa gtcaggatga
    31201 tgctatgccc gttgcccacg ttgccttgcc cgttccgctt cctcgtacct ttgactatct
    31261 gctgccagaa ggcatgacgg ttaaagctgg gtgtcgcgtg cgcgtgccgt ttggcaaaca
    31321 gcaggagcgc atcgggattg tggtatcagt tagcgatgcc agcgaactgc cgctcactga
    31381 gctaaaagcg gtagtcgaag tgctggatgg tgagccggtg tttactcact ccgtctggcg
    31441 attgctgcta tgggcggcag attactatca tcatccgatt ggcgatgtgc tgtttcatgc
    31501 cttgccgatt ttactacgcc aggggcggcc tgcggcgaac gcgccgatgt ggtactggtt
    31561 tgccactgaa caaggtcagg cggtggatct gaacagcctg aaacgctcac ccaagcaaca
    31621 acaggcgctg gcagcgttac ggcaaggcaa aatctggcgc gaccaggttg ccacgctcga
    31681 atttaatgat gccgcgttgc aggcgctgcg caaaaaaggt ctgtgtgatt tagcaagtga
    31741 aacaccagag tttagcgact ggcgaacgaa ctatgccgtt tctggtgagc ggttgcgatt
    31801 gaataccgaa caggccaccg ccgttggcgc aattcatagc gcggcagata ctttttctgc
    31861 ctggctgctg gcgggcgtta ccggttccgg taaaacggag gtttatctca gtgtactgga
    31921 aaacgtgctc gctcagggca aacaggcgct ggtgatggtg ccggaaatcg gcctgacacc
    31981 gcaaactatc gcccgttttc gtgaacggtt taatgcgccc gtggaagttc tgcattccgg
    32041 tctgaacgac agcgagcgtc tttcggcgtg gctgaaagcg aaaaatggcg aggcggcgat
    32101 tgtgatcggc acccgctccg cgctgtttac gccgtttaaa aatctcggcg tgattgtcat
    32161 tgacgaagag cacgacagtt cctacaagca gcaggaaggc tggcgctatc atgcccgcga
    32221 cctggcggtg tatcgtgcgc atagcgagca aatcccgatt attcttggct ccgcaacgcc
    32281 cgcgctggaa acgttgtgca acgtccagca gaaaaaatac cgcctgctgc gcctgacgcg
    32341 ccgggcgggg aatgcgcgtc cggcaattca acacgtgctg gatttaaaag gtcagaaggt
    32401 gcaggcaggt ctggctccgg cgttaatcac ccgtatgcgc cagcatttac aggccaacaa
    32461 tcaggttatc ctctttctta accgccgtgg ctttgcgcct gcgctactgt gccacgactg
    32521 cggctggatt gccgaatgcc cacgttgcga tcactactac acgctgcatc aggcgcaaca
    32581 gcatttgcgc tgtcaccact gtgacagtca gcgtccggtg ccgcgccagt gcccttcctg
    32641 cggttccacg cacctggtac ccgtggggct gggcaccgaa cagcttgaac agacgcttgc
    32701 gccgctgttc cctgatgtgc ccatttctcg tatcgaccgc gataccacca gccgcaaagg
    32761 ggcgctggaa cagcaactgg cggaagtaca tcgcggcggt gcgaggattt tgatcggcac
    32821 acaaatgctg gcgaaaggtc accactttcc ggatgtgacg ctggtggcgt tactggatgt
    32881 ggacggcgcg ctgttttctg ccgattttcg ctcggcagag cgtttcgctc agctttacac
    32941 ccaggtcgct ggtcgtgccg ggcgtgcggg taaacaaggc gaagtggtgc tgcaaacgca
    33001 ccatccggaa catcctctgt tgcaaacgtt gctctataaa ggctacgacg cctttgccga
    33061 acaggcgctg gctgagcgac ggatgatgca actgccgcca tggaccagcc atgtgattgt
    33121 gcgtgcggaa gatcataaca atcagcatgc gccgctgttc ctgcaacaac tgcgtaatct
    33181 gatcctctcc agtccgctgg cagacgacaa actgtgggtt ctcggtccgg ttccggcact
    33241 ggcacctaaa cgtggcggtc gctggcgctg gcagatattg ttgcagcacc cttcccgcgt
    33301 gcgcttgcaa cacatcatta gtggtacgct ggcgctcatc aatacaatac cggattcccg
    33361 caaggtgaaa tgggtactgg atgttgatcc gattgagggt taaaccgctt acgatgcgag
    33421 gcggatcgaa aaattcaata ttcatcacac ttttcatgaa aattctgtaa ccgttttcac
    33481 gcgctatctg ctaaaaatgt tgccgatgtg aagtaaacat ggatgtagta cgcctgacgt
    33541 accaggcgag gagtgagtgt gaaagcgaag aagcaggaaa ctgccgcgac catgaaagac
    33601 gttgccctca aggcaaaagt ctctacagcg actgtctccc gagcattaat gaatcccgat
    33661 aaagtctccc aggccacccg taatcgggtt gaaaaagcgg cccgggaagt gggttattta
    33721 ccgcagccta tggggcgcaa cgtcaagcgt aatgaatccc gcaccattct ggtgattgtc
    33781 ccggatatct gcgatccctt ctttagcgaa attattcgcg gtatcgaagt tacggcggca
    33841 aatcacggat atctggtgct gattggcgac tgtgcgcatc aaaatcagca ggaaaaaacc
    33901 tttatcgatt tgatcatcac caagcaaatt gatggcatgt tgctgctggg ttcaaggctg
    33961 ccgtttgatg ccagcattga ggaacagcgt aatctgccgc cgatggtgat ggcgaacgaa
    34021 tttgcaccgg aactggagct gcctaccgtt catatcgaca atctgaccgc cgcatttgat
    34081 gcagtaaatt atttatatga gcaagggcat aaacggattg gctgtatagc cggtcccgaa
    34141 gagatgccac tgtgtcacta ccgcctgcaa ggctatgttc aggcgctgcg tcgctgcgga
    34201 attatggttg atccacaata catcgcccgt ggcgacttca ccttcgaagc cggaagcaaa
    34261 gcaatgcaac agctgcttga tcttccacaa ccgcctactg cagtcttctg ccatagcgat
    34321 gtgatggcgc tcggcgcact ttctcaggca aaacgccagg ggctgaaagt cccggaagac
    34381 ctttccataa tcggttttga taacatcgac ctgacgcaat tttgtgatcc gccgctgaca
    34441 accatcgcgc agccgcgtta cgaaatcggt cgggaagcta tgctgttatt gcttgatcaa
    34501 atgcaggggc aacacgttgg cagtggctct cgtttaatgg actgcgaact tatcatccgg
    34561 ggatcaacac gcgcgttacc ttaaagtaaa cctttaagac tgccgtgact ggtcaaaggc
    34621 ccgccgctta agtaacatgg cgggctgacg aacgaataaa tacagcgaaa cgatagtggc
    34681 acaacgagat tatgtacgcc gcagccaacc ggcaccttcg cggcgaaaaa agagcacctc
    34741 acggaaaaag caacgaaatc tgcctgcggt ttctcccgct atggtcgcta ttgctgccgc
    34801 cgttcttgtg acctttatcg gtggtctgta cttcattacg catcacaaga aagaagagtc
    34861 cgagacgctg caaagccaga aagtgaccgg aaacggacta ccaccaaaac cagaagaacg
    34921 ctggcgctac attaaagagc tggaaagtcg ccagccgggt gtgcgtgcgc ccacagaacc
    34981 ttctgccggt ggtgaagtga aaacgccgga gcaactgaca ccagaacaac gtcagcttct
    35041 tgaacaaatg caggctgata tgcgccagca gccaacgcag ctggttgagg ttccgtggaa
    35101 tgaacagacg ccggaacagc gtcagcaaac gctacaacgc cagcgtcagg cgcagcaatt
    35161 agcggagcag caacgcctgg ctcagcagtc gcgtacgact gaacaaagct ggcagcagca
    35221 gacgcgtacg tcgcaagccg cgccggtaca ggcgcaaccg cgccagtcta aaccagcttc
    35281 tactcagcag ccgtaccagg atctgctgca aacccctgcg cacacgaccg cgcagtcgaa
    35341 gccgcagcaa gctgcgccgg ttgctcgtgc cgctgacgcg ccaaaaccga cggcggagaa
    35401 aaaagacgaa cgccgctgga tggtgcagtg cggttcgttc agaggcgcgg aacaggcaga
    35461 gacggtacgt gctcagctgg cgttcgaagg atttgactcg aaaatcacca ccaacaatgg
    35521 ctggaatcgt gtggtcattg gcccggtgaa aggcaaagag aacgcagaca gcaccctcaa
    35581 tcggttgaag atggcgggtc atacaaactg cattcggctc gccgccgggg gttgaaaccc
    35641 tcaaaatccc ccccatctat aattgcatta tgccccgtac ttttgtacgg ggtttgtact
    35701 ctgtattcgt aaccaagggg tcagctcgtg acaactatag taagcgtacg ccgtaacggc
    35761 catgtggtca tcgctggtga tggtcaggcc acgttgggca ataccgtaat gaaaggcaac
    35821 gtgaaaaagg tccgccgtct gtacaacgac aaagtcatcg cgggctttgc gggcggtact
    35881 gcggatgctt ttacgctgtt cgaactgttt gaacgtaaac tggaaatgca tcagggccat
    35941 ctggttaaag ccgccgttga gctggcaaaa gactggcgta ccgatcgcat gctgcgcaaa
    36001 cttgaagcac tgctggcagt cgctgatgaa actgcatcgc ttatcatcac cggtaacggt
    36061 gacgtggtgc agccagaaaa cgatcttatt gctatcggct ccggcggccc ttacgcccag
    36121 gctgcggcgc gcgcgctgtt agaaaacact gaacttagcg cccgtgaaat tgctgaaaag
    36181 gcgttggata ttgcaggcga catttgcatc tataccaacc atttccacac catcgaagaa
    36241 ttaagctaca aagcgtaagg atctcccatg tctgaaatga ccccacgcga aatcgtcagc
    36301 gaactggata agcacatcat cggccaggac aacgccaagc gttctgtggc gattgctctg
    36361 cgtaaccgct ggcgtcgcat gcagctcaac gaagagctgc gccatgaagt gaccccgaaa
    36421 aatatcctga tgatcggccc gaccggtgtc ggtaaaactg aaatcgcccg tcgtctggct
    36481 aagctggcga atgcgccgtt catcaaagtt gaagcgacca aatttaccga agtgggctac
    36541 gtcggtaagg aagttgattc tattattcgc gatctgaccg atgccgccgt gaaaatggta
    36601 cgcgtccagg ctatcgagaa aaaccgttat cgcgctgaag aactggcaga agaacgtatt
    36661 ctcgacgtgc tgatcccacc tgctaaaaac aactggggac agactgaaca gcagcaggaa
    36721 ccttccgctg ctcgtcaggc attccgcaaa aaactgcgtg aaggccagct tgatgacaaa
    36781 gaaatcgaga tcgatcttgc cgcagcaccg atgggcgttg aaattatggc tcctccgggc
    36841 atggaagaga tgaccagcca gctgcagtcc atgttccaga acctgggcgg ccagaagcaa
    36901 aaagcgcgta agctgaaaat caaagacgcc atgaagctgc tgattgaaga agaagcggcg
    36961 aaactggtga acccggaaga gctgaagcaa gacgctatcg acgctgttga gcagcacggg
    37021 atcgtgttta tcgacgaaat cgacaaaatc tgtaagcgcg gcgagtcttc cggtccggat
    37081 gtttctcgtg aaggcgttca gcgtgacctg ctgccgctgg tagaaggttg caccgtttcc
    37141 accaaacacg ggatggtcaa aactgaccac attctgttta tcgcttctgg cgcgttccag
    37201 attgcgaaac cgtctgacct gatcccggaa ctgcaaggtc ggctgccaat ccgcgttgaa
    37261 ctgcaggcac tgaccaccag cgacttcgag cgtattctga ccgagccgaa tgcctctatc
    37321 accgtgcagt acaaagcact gatggcgact gaaggcgtaa atatcgagtt taccgactcc
    37381 ggtattaaac gcatcgcgga agcggcatgg caggtgaacg aatctaccga aaacatcggt
    37441 gctcgtcgtt tacacactgt tctggagcgt ttaatggaag agatttccta cgacgccagc
    37501 gatttaagcg gtcaaaatat cactattgac gcagattatg tgagcaaaca tctggatgcg
    37561 ttggtggcag atgaagatct gagccgtttt atcctataat cgcgttcaat cattttcatc
    37621 attgtttgat ggggctgaaa ggccccattt ttattggcgc gtattatgac tgaacaacaa
    37681 attagccgaa ctcaggcgtg gctggaaagt ttacgaccta aaaccctccc cctcgccttt
    37741 gctgcaatta tcgtcgggac ggcgctggca tggtggcaag gtcacttcga tccgctggtc
    37801 gccctgctgg cactaattac cgccgggcta ttacagatcc tttctaacct cgccaatgat
    37861 tacggcgatg cggtaaaagg cagcgataaa cctgaccgca ttgggccgct acgcggcatg
    37921 caaaaagggg tcattaccca gcaaaagatg aaacgggcgc tcattattac cgtcgtgctc
    37981 atctgtctct ccgggctggc actggttgca gtggcatgcc atacgctggc cgattttgtc
    38041 ggtttcctga ttcttggcgg gttgtcgatc attgccgcta tcacctacac cgtgggcaat
    38101 cgtccttatg gttatatcgg tctgggtgat atttccgtac tggttttctt tggctggttg
    38161 agtgtcatgg ggagctggta tttacaggct catacattga ttccggcact gatccttccg
    38221 gcgaccgcat gcggcctgct ggcaacggca gtactgaata ttaataacct gcgtgatatc
    38281 aatagcgacc gcgaaaatgg caaaaacacg ctggtggtgc gcttaggtga agtgaacgcg
    38341 cgtcgttatc atgcctgcct gctgatgggc tcgctggtgt gtctggcgct gtttaatctc
    38401 ttttcgctgc atagcctgtg gggctggctg ttcctgctgg cggcaccatt actggtgaag
    38461 caagcccgtt atgtgatgcg ggaaatggac ccggtggcga tgcgaccaat gctggaacgt
    38521 actgtcaagg gagcgttact gactaacctg ctgtttgttt tagggatatt cctaagccag
    38581 tgggcagcat aactgacaaa tatcaattaa caattgatga ttttgccaac agcccacata
    38641 gcgcgatata ctgaaaattc tcgcagcaac tgaatgttaa gcctatgaaa tacgatactt
    38701 ccgagctttg tgacatctat caagaagatg ttaacgtcgt ggaaccgctg ttctccaact
    38761 ttggcggacg ggcgtcgttt ggcggacaaa taatcacggt aaaatgtttc gaggacaacg
    38821 ggttgctgta cgatctgctc gaacagaatg gccgtggtcg tgttcttgtc gtcgatggcg
    38881 gtggttctgt tcgtcgcgca ctggtcgatg ctgaactggc gcgtctggca gtacaaaatg
    38941 aatgggaagg tctggtcatt tacggcgcgg tgcgtcaggt agatgacctg gaagagttgg
    39001 atatcggcat ccaggcgatg gcggcaattc cggttggtgc cgctggcgaa ggcattggcg
    39061 aaagcgatgt ccgcgtcaat tttggcggtg tcaccttctt ctccggcgac catctttatg
    39121 ccgacaatac cgggattatt ctttcagaag atccgctgga tattgaatga taagaaaggc
    39181 accgcaaggt gccttttttc tgcgttacct gttggcctac acagtaaaga aattacgcga
    39241 aagatgaagc gtaatcagac ctcttccatg cgacccagca gggcctgcag acgttcctgc
    39301 cagccgttct gctgttcttt cagatggttg ttctcacgct ccagctcttc gcgctgatgc
    39361 tgggcatttt gaacttcctg cgacagtgag ttgttttttt ctttcagctc ttcgatttcc
    39421 atctgcaaca gagtgatggt atcaatcgcc tgctgtactt ttgcttccag tttatcaaac
    39481 acttctaatg acattgtcat acctctcctg aattgcaagg cgttgatgga taaaaaatcc
    39541 tcgtcccgat taccggtgac gccttaataa atacgagcgc actttagtta gctccgattg
    39601 tatgaagccg cgccatcgct gtccagcggc acgccttgca gattacggtt tgccacactt
    39661 ttcatccttc tcctagtgac ataatccaca tcaatcgaaa atgttaataa atttgttgcg
    39721 cgaatgatct aacaaacatg catcatgtac aatcagatgg aataaatggc gcgataacgc
    39781 tcattttatg acgaagcaca cacattttaa gttcgatatt tctcgttttt gctcgttaac
    39841 gataagttaa cagcatgcct acaagcatcg tggaggtccg tgactttcac gcatacaaca
    39901 aacattaact cttcaggatc cgattatgag tcaaacatca accttgaaag gccagtgcat
    39961 tgctgaattc ctcggtaccg ggttgttgat ttttttcggt gtgggttgcg ttgcagcact
    40021 aaaagtcgct ggtgcgtctt ttggtcagtg ggaaatcagt gtcatttggg gactgggggt
    40081 ggcaatggcc atctacctga ccgcaggggt ttccggcgcg catcttaatc ccgctgttac
    40141 cattgcattg tggctgtttg cctgtttcga caagcgcaaa gttattcctt ttatcgtttc
    40201 acaagttgcc ggcgctttct gcgctgcggc tttagtttac gggctttact acaatttatt
    40261 tttcgacttc gagcagactc atcacattgt tcgcggcagc gttgaaagtg ttgatctggc
    40321 tggcactttc tctacttacc ctaatcctca tatcaatttt gtgcaggctt tcgcagttga
    40381 gatggtgatt accgctattc tgatggggct tatcctggcg ttaacggacg atggcaacgg
    40441 tgtaccacgc ggccctttgg ctcccttgct gattggtcta ctgattgcgg tcattggcgc
    40501 atctatgggc ccattgacag gttttgccat gaacccagcg cgtgacttcg gtccgaaagt
    40561 ctttgcctag ctggcgggct ggggcaatgt cgcctttacc ggcggcagag acattcctta
    40621 cttcctggtg ccgcttttcg gccctatcgt tggcgcgatt gtaggtgcat ttgcctaccg
    40681 caaactgatt ggtcgccatt tgccttgcga tatctgtgtt gtggaagaaa aggaaaccac
    40741 aactccttca gaacaaaaag cttcgctgta atatgactac gggacaatta aacatgactg
    40801 aaaaaaatat atcgttgcgc tcgaccaggg caccaccagc tcccgcgcgg tcgtaatgga
    40861 tcacgatgcc aatatcatta gcgtgtcgca gcgcgaattt gagcaaatct acccaaaacc
    40921 aggttgggta gaacacgacc caatggaaat ctgggccacc caaagctcca cgctggtaga
    40981 agtgctggcg aaagccgata tcagttccga tcaaattgca gctatcggta ttacgaacca
    41041 gcgtgaaacc actattgtct gggaaaaaga aaccggcaag cctatctata acgccattgt
    41101 ctggcagtgc cgtcgtaccg cagaaatctg cgagcattta aaacgtgacg gtttagaaga
    41161 ttatatcagc agcaataccg gtctggtgat tgacccgtac ttttctggca ccaaagtgaa
    41221 gtggatcctc gaccatgtgg aaggctctcg cgagcgtgca cgtcgtggtg aattgctgtt
    41281 tggtacggtt gatacgtggc ttatctggaa aatgactcag ggccgtgtcc atgtgaccga
    41341 ttacaccaac gcctctcgta ccatgttgtt caacatccat accctggact gggacgacaa
    41401 aatgctggaa gtgctggata ttccgcgcga gatgctgcca gaagtgcgtc gttcttccga
    41461 agtatacggt cagactaaca ttggcggcaa aggcggcacg cgtattccaa tctccgggat
    41521 cgccggtgac cagcaggccg cgctgtttgg tcagttgtgc gtgaaagaag ggatggcgaa
    41581 gaacacctat ggcactggct gctttatgct gatgaacact ggcgagaaag cggtgaaatc
    41641 agaaaatggc ctgctgacca ccatcgcctg cggcccgact ggcgaagaga actatgcgtt
    41701 ggaaggtgcg gtgtttatgg caggcacatc cattcagtgg ctgcgcgatg agatgaagtt
    41761 gattaacgac gcctacgatt ccgaatattt cgccaccaaa gtgcaaaaca ccaatggtgt
    41821 gtatgtggtt ccggcattta ccgggctgag tgcgccgtac tgggacccgt atgcgcgcgg
    41881 ggcgattttc ggtctgactc gtggggtgaa cgctaaccac attatacgcg cgacgctgga
    41941 gtctattgct tatcagacgc gtgacgtgct ggaagcgatg caggccgact ctggtatccg
    42001 tctgcacgcc ctgcgcgtgg atggtggcgc agtagcaaac aatttcctga tgcagttcca
    42061 gtccgatatt ctcggcaccc gcgttgagcg cccggaagtg cgcgaagtca ccgcattggg
    42121 tgcggcctat ctcgcaggcc tggcggttgg cttctggcag aacctcgacg agctgcaaga
    42181 gaaagcggtg attgagcgcg agttccgtcc aggcatcgaa accactgagc gtaattaccg
    42241 ttacgcaggc tggaaaaaag cggtcaaacg cgcgatggcg tgggaagaac acgacgagta
    42301 atgtcaggca cctgaatcga ttgcctgatg ctgatcgcct gatgcgacgc ttacgcgtct
    42361 tatcaggcct acgagccaat gccattttgt gtaggcggga taaggcgttc acgccgcatc
    42421 cggcatcacg cagactctga aaccgtgcgg gggcaacccc gcacacatca ataatcccac
    42481 ccttcccctg tgctacactt cgcgccattc cttactgctt agagtttgct atgagacgag
    42541 aacttgccat cgaattttcc cgcgtcaccg aatctgcggc gctggctggc tacaaatggt
    42601 taggacgcgg cgataaaaac accgcggacg gcgcggcggt aaacgccatg cgtattatgc
    42661 tcaaccaggt caacattgac ggcaccatcg tcattggtga aggtgaaatc gacgaagcac
    42721 cgatgctcta cattggtgaa aaagtcggta ctggtcgcgg cgacgcggta gatattgctg
    42781 ttgatccgat tgaaggcacg cgcatgacgg cgatgggcca ggctaacgcg ctggcggtgc
    42841 tggcagtagg cgataaaggc tgcttcctca atgcgccgga tatgtatatg gagaagctga
    42901 ttgtcgggcc gggagccaaa ggcaccattg atctgaacct gccgctggcg gataacctgc
    42961 gcaatgtagc ggcggcgctc ggcaaaccgt tgagcgaact gacggtaacg attctggcta
    43021 aaccacgcca cgatgccgtt atcgctgaaa tgcagcaact cggcgtacgc gtatttgcta
    43081 ttccggacgg cgacgttgcg gcctcaattc tcacctgtat gccagacagc gaagttgacg
    43141 tgctgtacgg tattggtggc gcgccggaag gcgtggtttc tgcggcggtg atccgcgcat
    43201 tagatggcga catgaacggt cgtctgctgg cgcgtcatga cgtcaaaggc gacaacgaag
    43261 agaatcgtcg cattggcgag caggagctgg cacgctgcaa agcgatgggc atcgaagccg
    43321 gtaaagtatt gcgtctgggc gatatggcgc gcagcgataa cgtcatcttc tctgccaccg
    43381 gtattaccaa aggcgatctg ctggaaggta ttagccgcaa aggcaatatc gcgactaccg
    43441 aaacgctgct gatccgcggc aagtcacgca ccattcgccg cattcagtcc atccactatc
    43501 tggatcgcaa agacccggaa atgcaggtgc acattctctg agtgatttga tcgattgagc
    43561 cttccagtcc ttcgggactg gaattttttt gttcggagaa cgaagataag gcaagtcaat
    43621 caaaacagga gaaaaacatg gctgattggg taacaggcaa agtcactaaa gtgcagaact
    43681 ggaccgacgc cctgtttagt ctcaccgttc acgcccccgt gcttccgttt accgccgggc
    43741 aatttaccaa gcttggcctt gaaatcgacg gcgaacgcgt ccagcgcgcc tactcctatg
    43801 ttaactcgcc cgataatccc gatctggagt tttacctggt caccgtcccc gatggcaaat
    43861 taagcccacg attggcggca ctgaaaccgg gcgatgaagt gcaggtggtt agcgaagcag
    43921 ctggcttctt tgttctggat gaagtaccgg actgcgaaac gctatggatg ctggcaaccg
    43981 gtacagcgat tggcccttat ttatcgattc tgcaactagg caaagattta gatcgcttca
    44041 aaaatctggt cctggttcac gccgcacgtt atgccgccga cttaagctat ttgccactga
    44101 tgcaagaact ggaaaaacgc tacgaaggca aactgcgcat tcagacggtg gtcagtcggg
    44161 aaacggcagc ggggtcgctc accggacgga taccggcatt aattgaaagt ggggaactgg
    44221 aaagcgcgat tggcctgccg atgaataaag aaaccagcca tgtgatgctg tgcggcaatc
    44281 cacagatggt gcgcgataca caacagttgc tgaaagagac ccggcagatg acaaaacatt
    44341 tacgtcgccg accgggccat atgacagcgg agcattactg gtaagcggtt acttatcgat
    44401 aaacggcacg atgagcaaat ccgcactcat cttattgatc atcccgcgat atgccggcat
    44461 caaacggttg ataaatgagt gatgatgacc acagacaagg aggtcgcact gctctttttg
    44521 cataatttcc agcagtgttt ccggcatttc tccgcgttca atacgcagtt ttgtcttcgg
    44581 ccattgaata tttttcgtca gtttatacag cttgttatcc gacttattct tcaacaattg
    44641 aagaatatct tctgttgcag ggaagtagat acccggatac aactcgctta agccatcatc
    44701 aatatgaatt aacgtcaggt gagcgtcatt atgtctggcg agctccaggg ctttattcac
    44761 cagtaaggca tcttcttcat tcccggaaat tgccacgcca atgtgtttat aagccatgtt
    44821 taactccttc taaagcaact ccatcaagct aacgaacgca gtgataactc aaaaataatc
    44881 atctcggcct ggcatgagaa agtgaaggcc gcatcaagct caacttgccc ctccagatcc
    44941 ttaatcttat aggccacgct ggccccttca tctgcagaga atgcagcggc ggcaagctct
    45001 ttcagtttgg tcataaaaga ctctgcttct tcgcgggtgg caaaaaagcg cgaaaactta
    45061 ctggtacagt tgtcgttatc aatcaccgta ccgatatcaa tcgcacatcc tttagtactg
    45121 catttatcta cgacatcttt catagggcac ctctgtatat tcgcctctct gtcgattacg
    45181 acttaagaat aatgcgccgt taagggaaga acaaacaagt aatgatctta tggattacca
    45241 ttttttttga tattgcttaa ggtgaagagt tcagaaggat tttccgggta ttgtcatttg
    45301 catcgtggaa ataacaatcc cggaaaaaaa tagcagtaag agatatatta aaggttaatt
    45361 acttgattta ttgtcggctt tatacttcac atcctgagta tctttaccat atttattttc
    45421 gccttgtgtg ccaacaaacg caccaagatc gataagcatc atcaccagaa tcaacgtcgg
    45481 gacaaaacgc cccaccgccc attgccagac acccggtaaa atcgctcagt tacccgccag
    45541 cagcatccac gccacaatca tcagaaatgc ccatgcgccg gaacgcccgc gatcatgcaa
    45601 gcgcttaaca gttactgccg ctgttggcca gagcaagcac acaaggcaaa acgccgcggt
    45661 ctgaatatcg agtagattct tacccgccag tgagaaaagc accagcatgc ctgcgaacca
    45721 caggcctatc caaatccaga aatcacggcg tccaatacgc cctttaaatg agaataacca
    45781 ttgctgtatg gtcatgtaag ttccttgatg gttgtctttt ccaggattct acccttttga
    45841 caagggcgac agatatcgtt ttaatcggag ccagtcataa caaaaggtac tgtcaatgaa
    45901 gccagggtgt acgctgtttt ttctcttatg ttctgcatta accgttacaa caacggcgca
    45961 tgcacaaaca ccagatacgg caacgaccgc gccttatctg ctggctggag cccctacttt
    46021 cgatctctcc atcagccagt ttcgagaaga ctttaacagc cagaatccca gcctgccact
    46081 gaacgaattt cgtgccatcg acagcagtcc cgacaaagcc aatctcactc gtgctgcaag
    46141 taaaattaac gagaacttgt atgcttctac agcgctggag cgcggtacct taaaaatcaa
    46201 aagcattcaa atgacctggc tacccatcca ggggccagag caaaaagcag cgaaagcgaa
    46261 agctcaggaa tacatggcag cagtgatccg cacactcacc ccattaatga ccaaaacaca
    46321 aagccagaaa aaactgcagt cgctactaac ggcggggaaa aacaaacgtt attacaccga
    46381 gacagaaggt gcactgcgtt atgttgtcgc ggacaacggc gaaaaggggc tgaccttcgc
    46441 tgttgaaccg attaagctgg cgctatctga atcgcttgaa ggtttgaata aatgacaaaa
    46501 agcaaagcct ttgtgccgat gaatctctat actgtttcac agacctgctg ccctgcgggg
    46561 cggccatctt cctttattcg cttataagcg tggagaatta aaatgcgaca tcctttagtg
    46621 atgggtaact ggaaactgaa cggcagccgc cacatggttc acgagctggt ttctaacctg
    46681 cgtaaagagc tggcaggtgt tgctggctgt gcggttgcaa tcgcaccacc ggaaatgtac
    46741 atcgatatgg cgaagcgcga agctgaaggc agccacatca tgctgggtgc gcaaaacgtg
    46801 gacctgaacc tgtccggcgc attcaccggt gaaacttctg ccgctatgct gaaagacatc
    46861 ggcgctcagt acatcatcat cggccactct gaacgtcgta cttaccacaa agagtctgac
    46921 gaactgattg cgaaaaaatt cgcggtgctg aaagagcagg gtctgactcc ggttctgtgc
    46981 atcggtgaaa ccgaagctga aaacgaagcg ggaaaaactg aagaagtttg cgcacgtcag
    47041 atcgacgcgg tactgaaaac tcagggtgct gcggcattcg aaggtgcggt tatcgcttat
    47101 gaacctgtat gggcaatcgg tactggcaaa tctgcaactc cggctcaggc acaggctgtt
    47161 cacaaattca tccgtgacca catcgctaaa gttgacgcta acatcgctga acaagtgatc
    47221 attcagtacg gcggctctgt aaacgcgtct aacgctgcag aactgtttgc tcagccagac
    47281 atcgacggcg cgctggttgg cggtgcttct ctgaaagctg acgctttcgc agtgatcgtt
    47341 aaagctgcag aagcggctaa acaggcttaa gtctgacagg tgccggattt catatccggc
    47401 acttactttc cttaactcct cgccttaacg caaaatctca cactcatgat cctggatttc
    47461 ctcggctgaa gcacggttca gcgtcagtag atttcgttgt gtcgccagca acacaaacga
    47521 attatcactc tgccgtacca tcgccagccc gtagcttccc atatgttccc gcgcctcagg
    47581 cacttcttct gcaagcatca taaatgggct gcgttgtacc agttcgcttt ccgttacccg
    47641 acgcgccagg tattcatgcc cgcgcaaacc acctggcagt ggcaaccagc ggctgctgat
    47701 gttcgccaga tttttatcca gttgttcgcg cacatcagga cgaatacaag agatatgaat
    47761 atgaaaatgg ttttgcgtac gcccggtgcg ggagttgatc gccaaagaaa ccgcgcgatc
    47821 gggaaccggc tggccgtatt ttttgctcat aaaatcacgc gcctgccagg ccaaccaaaa
    47881 gaagttcggc gttgaaggat cggtcaacaa aggactttca gtaccgttaa tacgatacgt
    47941 tgacatcaac agatattgca gtggaccatt aagatctttt aaaaccacgt atccggcatt
    48001 gggtttgact tccgcacatg gcgaaggatt ttgattttgc tgctgattgg gcaaacattc
    48061 ctcaaggaca atcttacgta atgtatccga ctcttcaccg gttaatttcc agtaaccaat
    48121 accggcagcc acaacggcga taactatcat caccaaaaaa agaagacccg cttttttcat
    48181 ctttttttcc ctgtacctca aagagatgca agggtaacgc aaaatcgtga caaataaaaa
    48241 acccggtcgc gagaaaccac cgggtttgat aattatccta gggaaatcag cgtttgctga
    48301 tctgatcgaa cgtaccgccg ttagcaaaat gctctttttg cgctttcgtc cagccgccga
    48361 actcttcatc aatggtgaat aacttcagct ttggaaacgc attttcgtac tttttcgcta
    48421 cttcagcgtc gcgcggacgg tagtagtttt tcgcggcaat ttcctgacct tctggcgagt
    48481 agagatattt caggtaggct tccgccacct ctttggtgcc ttttttctcg accactttat
    48541 cgaccaccga caccgttggc tctgcgagga tagactcact cggcgtgacg atttcgaatt
    48601 tatctttccc cagttcattc gctgccagca gagcttcgtt ttcccaggcg atcagtacat
    48661 cgccaatccc gcgctcgaca aaggtgttag tggagccgcg cgcgccagaa tccagaactt
    48721 cgacgttttt atacagtgcc cgaacaaaat cctgtgcttt tgcctgatcg ttgttgttgt
    48781 gatgcagcgc gtagccccag gcagccaggt agttccagcg tgcgccacca gagcttttcg
    48841 gattaggcgt gatcaccgaa acacccggtt taatcagatc gttccagtca tggatctgct
    48901 tcggattgcc cttacgcacc aggaaaacaa tggtggaagt gtacggtgcg gagttatccg
    48961 gcagacgttt gatccactct ttatcaatcc gcccgcgttc cgcaatcgcg tccacgtcat
    49021 aggccagagc cagcgtgaca acatcagctt caataccgtt gattaccgac gtcgcttgtt
    49081 tacctgaacc accgtgtgac tgacgaatca ccacgttatc accagtttgc tgtttccagt
    49141 gggcgctgaa tgccttgttg tactgttcgt acaattcgcg cgttggatca tatgaaacgt
    49201 taagaagctg aatatccttt gccataacgc tggttgccgc cagcaaaaat gttaacccta
    49261 cgccccactt gttcatcgcc cgactctctt atgttgtgtt gtgatgagca aagcgtgcca
    49321 gaaggttaac caaacattaa agaataaaaa aagattggct ataacttgcg ggtatatgtt
    49381 gagggattaa aaaggcggaa gaaatccgcc tcatattgct gacaaagtgc gctttgtcca
    49441 tgccggatgc ggcgcgaacg ccttatccgg cctacaaaag tttgcaaatt caataaattg
    49501 cagaattcat gtaggcctga taagcgaagc gcatcaggca tttttgcttc tgtcatcggt
    49561 ttcaggctaa aggaatctgc ctttttccga aatcattaat acagtttttt cgcgcagtcc
    49621 agccagtcac ctttgaacgg acgcttcatg ttttcgatag cgtcgatgat gtcgtggtga
    49681 accagctgtt cgttctggat acctacacaa cgaccgccgt aacctgccag cagcagatcg
    49741 atagcgtaag cgcccatacg ggaagccaga atacggtcgt aaggcaccgg agaaccaccg
    49801 cgctggatgt ggcccagcac agttgcgcgg gtttcacgac cggtttcttt ctcgatgaaa
    49861 tgcgccagtt cgtcaacatc acacatatgt tcggtaatcg ccacgatcgc gtgtttttta
    49921 cctttcgcga tacccgcttt gatttcgttt accaggtctt cacggctgaa ttcaacttcc
    49981 ggaactacaa cgaattcaca gccaccggca atggccgcag ccagggtcag atcgccacaa
    50041 taacggccca tcacttccac cacggaaata cgctggtgag aagaagaggt gtcacgcaga
    50101 cggtcgatcg cttctacaac ggtgctcagc gcagtgaaga aaccgatagt gtagtcagtg
    50161 cctttgatgt cgttgtcgat agtgcccggc agaccgatgc acgggaagcc catttcggtc
    50221 agacgcattg cacccatgta ggaaccgtca ccgccgataa ccaccagcgc gtcgatacca
    50281 cgttttttca ggttttcgat agccacggcg cggatgttct cgtcgcggaa ttccgggaaa
    50341 cgcgcagaac cgaggaacgt accgccacgg ttgatcatgt cagacacgct gtaacggtct
    50401 agctgtacca tacggtcttc atacagaccc agatagccgt cataaatacc cattacttcc
    50461 agaccttctg tcagcgcaga acgaacaacc ccgcgaattg cggcgttcat gcctggcgca
    50521 tcaccgccgc ttgtcaacac acctattttc ttaatcatga ctacctctga actttggaat
    50581 gcaaaatgaa atctgttgcc ggaagtcttc ttgcacatcg aagtgatcca acgaatgtgc
    50641 aaatagtata acaatcactt cctgctgaat tgattcaggt caggccaaat ggcggtattt
    50701 tatacacaaa atgcgggtct ggctctcttt tatactgatt atgaaagcat agaccgttta
    50761 ccctccctgg gtacgacgga acaggggtcc tgatggataa tcacatccga tcccggaaaa
    50821 cgccgtaaaa tagcctgctc tacctgatcc gccaccatat gtgcctgaac caaaggcaga
    50881 gagtcttcca tttccaaatg aatctgaata aagcgggtcg gccctgactg ccgcgtgcga
    50941 agatcgtgag cgccgctaac acccggccag gaagtcacga tatcaataat ttcttgccgt
    51001 tcttcatcag gcaatgcgcg atccagtaat gactgtaccg cctcatatcc catgcgtaac
    51061 gcgctatata aaatatagat gccgattccc aatgcaaaca gagcatcggc gcgatgccag
    51121 ccgtaccaag acaaccccag cgccagcaga attgcgccgt tcatcataac atcagactgg
    51181 taatgtagca tatcagcccg caccgcctgg ctttgcgtcc ggcgcaccac ccaccgctga
    51241 aacgagacaa ggataatcgt acaaattagc gccacaattg tcacgataac cccgacgcct
    51301 ggatctgtca tcggtgttgg agataccaga tgttgaatac ccgtcaaaaa caggaatagt
    51361 gccgaaccgg agataaacat actttgcgcc agcgccgcga gggactcagc tttaccgtga
    51421 ccaaacgagt gattatcgtc ggcaggttgc agggaatatc gcaccaccag taaattcgtc
    51481 aacgacgcgc cgatatccac cagcgaatcc accagcgcgg cgagaatact caccgacccg
    51541 gtataccacc atgcaaaaat tttaatcagc aatagcaacg aagccatcgc cgtcgcagca
    51601 atcgccgccc gactgaccag ccgtccataa gattgattca taaatactcc cgctatcaac
    51661 tgacgctagt ataacggaag caaatcatct gcaatgcatt aagcagcagg caaattgagg
    51721 ataaaaaaaa ccccacatca tgtgggggaa gacagggatg gtgtctatgg caaggaaaac
    51781 agggtttact actgggaacg tgagttgcta ctactcaata gcttcaacga tgaacttttt
    51841 tgccattgcg tcacgtcacg caactgctcc attcgttgtt gatgtttctc gtttaaaacc
    51901 gcttgctgct ccggcgttaa caggcgatac atttggttgc ggacttttgc catctcaacc
    51961 tgacgagcaa tttgctcatt cgccattttt tctgcctgtg cgcgcacagc gttttcatca
    52021 aaattttctg cggtgacaag gcgatgcatt gtctccagtt cgctaacatt aacaggaggc
    52081 tgttcgtgcc gggcctgttg cataagatct cgcatctgct gacgctgatg ttcggttaaa
    52141 cttatgccgt cgaacatatg gctctgcgta ctgcgctgcg taagttcttc acccggatgc
    52201 cagttatcgc ctgaaccgac ttcagcagcg tggcttaatg aactgactgc cagcgttgag
    52261 gccatgacgg cagcggtaac tatgcgcatc atttgctccc aaaatctttc tgtcgcgatt
    52321 caacgataga gagtttacga ttcaggctgc aaacatgcgt cagggggtgt aaaacaacgt
    52381 aaagtcatgg attagcgacg tctgatgacg taatttctgc ctcggaggta tttaaacaat
    52441 gaataaaatc ctgttagttg atgatgaccg agagctgact tccctattaa aggagctgct
    52501 cgagatggaa ggcttcaacg tgattgttgc ccacgatggg gaacaggcgc ttgatcttct
    52561 ggacgacagc attgatttac ttttgcttga cgtaatgatg ccgaagaaaa atggtatcga
    52621 cacattaaaa gcacttcgcc agacacacca gacgcctgtc attatgttga cggcgcgcgg
    52681 cagtgaactt gatcgcgttc tcggccttga gctgggcgca gatgactatc tcccgaaacc
    52741 gtttaatgat cgtgagctgg tggcacgtat tcgcgcgatc ctgcgccgtt cgcactggag
    52801 cgagcaacag caaaacaacg acaacggttc accgacactg gaagttgatg ccttagtgct
    52861 gaatccaggc cgtcaggaag ccagcttcga cgggcaaacg ctggagttaa ccggtactga
    52921 gtttaccctg ctctatttgc tggcacagca tctgggtcag gtggtttccc gtgaacattt
    52981 aagccaggaa gtgttgggca aacgcctgac gcctttcgac cgcgctattg atatgcacat
    53041 ttccaacctg cgtcgtaaac tgccggatcg taaagatggt cacccgtggt ttaaaacctt
    53101 gcgtggtcgc ggctatctga tggtttctgc ttcatgatag gcagcttaac cgcgcgcatc
    53161 ttcgccatct tctggctgac gctggcgctg gtgttgatgt tggttttgat gttacccaag
    53221 ctcgattcac gccagatgac caagcttctg gatagcgaac agcgtcaggg gctgatgatt
    53281 gagcagcatg tcgaagcgga gctggcgaac gatccgccca acgatttaat gtggtggcgg
    53341 cgtctgttcc gggcgattga taagtgggca ccgccaggac agcgtttgtt attggtgacc
    53401 accgaaggcc gcgtgatcgg cgctgaacgc agcgaaatgc agatcattcg taactttatt
    53461 ggtcaggccg ataacgccga tcatccgcag aagaaaaagt atggccgcgt ggaactggtc
    53521 ggtccgttct ccgtgcgtga tggcgaagat aattaccaac tttatctgat tcgtccggcc
    53581 agcagttctc aatccgattt cattaactta ctgtttgacc gcccgctatt actgctgatt
    53641 gtcaccatgt tggtcagtac gccgctgctg ttgtggttgg cctggagtct ggcaaaaccg
    53701 gcgcgtaagc tgaaaaacgc tgccgatgaa gttgcccagg gaaacttacg ccagcacccg
    53761 gaactggaag cggggccaca ggaattcctt gccgcaggtg ccagttttaa ccagatggtc
    53821 accgcgctgg agcgcatgat gacctctcag cagcgtctgc tttctgatat ctctcacgag
    53881 ctgcgcaccc cgctgacgcg tctgcaactg ggtacggcgt tactgcgccg tcgtagcggt
    53941 gaaagcaagg aactcgagcg tattgaaacc gaagcgcaac gtctggacag catgatcaac
    54001 gatctgttgg tgatgtcacg taatcagcaa aaaaacgcgc tggttagcga aaccatcaaa
    54061 gccaaccagt tgtggagtga agtgctggat aacgcggcgt tcgaagccga gcaaatgggc
    54121 aagtcgttga cagttaactt cccgcctggg ccgtggccgc tgtacggcaa tccgaacgcc
    54181 ctggaaagtg cgctggaaaa cattgttcgt aatgctctgc gttattccca tacgaagatt
    54241 gaagtgggct ttgcggtaga taaagacggt atcaccatta cggtggacga cgatggtcct
    54301 ggcgttagcc cggaagatcg cgaacagatt ttccgtccgt tctatcggac cgatgaagca
    54361 cgcgatcgtg aatctggcgg tacaggattg gggctggcga ttgttgaaac cgccattcag
    54421 cagcatcgtg gctgggtgaa ggcagaagac agcccgctgg gcggtttacg gctggtgatt
    54481 tggttgccgc tgtataagcg gagttaaact ccgcatttgt aggcaggata aggcgtttac
    54541 gccgcatccg gcatttgagc aggatgcctg atgcgacgct gatagcgtct tatcaggcct
    54601 acactccggg ggttattttc cccacaaccg gcgggaaaaa tcttcgatct tgccgctcag
    54661 tcggcgcttt tgcatcgtcc gcgtccagct tttcgataac ccggcggcgg agagtaaacg
    54721 gtgatactga tcgtcatcaa acggcatatg ccatgcgatg gcggcagcct cctgcacggt
    54781 cacatcgctg acacgggaaa ccaactccag cggcgcgtcc gcagacactt tccctggcgc
    54841 tatcacgcta tacaaccacc ccaccttacc tgtgttttgc atcaactgcg ctatatcgct
    54901 gatatcaaaa tggtaattga gtttatagca aggcgagcgc ggctggctga cctgaattaa
    54961 tgcctctccc cagcggaaaa tatcgcccat atgaacatta ctttccgtga gaccgtcggt
    55021 tgagagattt tcgccaaacg caggcgcaac aaacaactcc gcctgttccg gaaattcccg
    55081 cacccagtag agatagtgct cacgaggata atgacacagc gctctgtctg gcccgccgtg
    55141 aacctttttc tccgcctgct cgtcaccttc cagccccagc tccgtcagca tcaactcacc
    55201 atcaacctgg attttggcaa ttgcgctggg tttgccttcg ggataagcct gaatcttgcc
    55261 tgtgtataca tcaaccggat atcgcatctg ttccctctcc acgagaaatt tccccattag
    55321 accatattta ttttatgtca gccgtgctgt tttcgcggta agtatccagg caggaactca
    55381 atagcaaacc ggacgaacgt tagttcatcc ggctgtgggg attaaggaag caggtttatt
    55441 tcaccgtacc taaaatttcg attttcgctg ctctggcttt gactttacgt gaccagatag
    55501 aggtaagaat tggcaccaga atcgaagtca caatgaccgc cgtcgctacc agcgaagttg
    55561 ctgccggagc catcggttta aacgcaggca ccatttctgc aatcagcaca ggtgtcgcta
    55621 ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt gccatcgcca ccgccaatca
    55681 atttatcggc gataatcaac ggaataccgg tcacgataat tattgccaca cccaacagga
    55741 tccccagcaa accagtctgg gcaatcacag tcaaatcaat ggtattgccc agcgcgaagg
    55801 caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa ttctcgtaat tcagggtcaa
    55861 gattccccag ggcaaagccc accaggaacg gtaatactgc gccgacgaaa acatgcggtt
    55921 caaacgaggc aatcccggca gtgcccagaa taatcatcgt catgagcggc ccggactcca
    55981 acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc gtactgctgc atgatggaag
    56041 cgtaaagtcc gccgttggtc atatccatcg ccgccaccag cgccagcgtt gaaagtccgg
    56101 caaagaatcc aacttcaaca ccatgttccg gaataatgcg cgaggcaatt gccgcaacca
    56161 cccacgcgac ggcaatttta gttaccacca gcgtaccgga tttacgcagt accgttcccg
    56221 tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca caccgccaga atgggcaccg
    56281 taccggtaat catcccgttg gtgaatgatc caaaatattt ccccgccccc ggcgagaagg
    56341 tgtggcacag tgcgccaagg aatagcggga cgagcatcat cccccccggg attttctcaa
    56401 tcgagcgttt tatctgcatt tccatcacct gtcggcatat ttttaagggc tgatgcgtaa
    56461 ttcctcaatg gagcatcagt gttgatggaa acgaagatag acattcattt aatcaataaa
    56521 agtgatcaaa accacatcaa ttgaaacgct gttttatttt tataggatca ttgtttttat
    56581 tttggaattg atcacaaaaa aaccaccgcc gttggcgatg gttcattttt taagctgata
    56641 tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt attttttcgc cgcaaaacgt
    56701 gccgctgctt cgtcccagtt caccacgttc cagaactctt tgatgtagtc cggacggcgg
    56761 ttctggaatt tcaggtagta agcatgttcc cacacatcca ggcccagaat cgggaagccg
    56821 gaagcgccag aaatagcttc acccatcagc ggagaatcct ggttagcagt agaaaccacc
    56881 gccagtttat cgcctttcag caccagccat gcccagccgg aaccaaagcg ggaagctgcc
    56941 gctttttcaa attctgcttt gaagttatca acggagccga agtcacgttc gatagccgct
    57001 ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt tccagaacag gctgtggtta
    57061 gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt ctgctggcag ctggtccagt
    57121 ttggtgatca gctcttcaac cggcaggttg gcaaattctg gcaggctttc cagcgccgcg
    57181 ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt ggatttccat ggtctgctta
    57241 tcgaagtgcg gttccagggc atcgtaagca tacggcaggg atggcagggt atagctcata
    57301 ttcatctcca gtattgtcgg gcggccgatt gttaatgccg cgtaagcagt tggtttatta
    57361 tagttaatta aatgatattg aaaatgatta tcaatgccgc acttttcgta agggtatggt
    57421 tttgcaggaa aatgcccgag atgtgaagca aatcacccac ttaatgccgt gattgccagt
    57481 aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca gcacgatttt tacactcatc
    57541 tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag aagatgagta acgcgattac
    57601 gatggggata ttttggcatt tgatcggcgc ggccagtgca gcctgttttt acgctccgtt
    57661 caaaaaagta aaaaaatggt catgggaaac catgtggtca gtcggtggga ttgtttcgtg
    57721 gattattctg ccgtgggcca tcagcgccct gttactaccg gatttctggg cgtattacag
    57781 ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc ggcgctatgt gggggatcgg
    57841 taatatcaac tatggcctga ccatgcgtta tctcggcatg tcgatgggaa ttggcatcgc
    57901 cattggcatt acgttgattg tcggtacgct gatgacgcca attatcaacg gcaatttcga
    57961 tgtgctgatt aacaccgaag gcggacgcat gacgttactc ggcgttctgg tggcgctgat
    58021 tggcgtaggg attgtaactc gagccgggca gttgaaagag cgcaagatgg gcattaaagc
    58081 cgaagagttc aatctgaaaa aagggctggt gctggcggtg atgtgcggca ttttctctgc
    58141 cgggatgtcc tttgcgatga acgccgcaaa accgatgcat gaagccgctg ccgcacttgg
    58201 cgtcgatcca ctgtatgtcg ctctgccaag ctatgttgtc atcatgggcg gcggcgcgat
    58261 cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg aaggatttgt cgctaaaagc
    58321 cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg ttactctcgg cactgggcgg
    58381 gttgatgtgg tatctgcaat tctttttcta tgcctagggc cacgcccgca ttccggcgca
    58441 gtatgactac atcagttgga tgctgcatat gagtttctat gtattgtgcg gcggtatcgt
    58501 cgggctggtg ctgaaagagt ggaacaatac aggacgccgt ccggtaacgg tgttgagcct
    58561 cggttgtgtg gtgattattg tcgccgctaa catcgtcggc atcggcatgg cgaattaatc
    58621 tttctgcgaa ttgagatgcg ccactggctg ggcgtcatcc cggtttcccg ggtaaacacc
    58681 accgaaaaat agttactatc ttcaaagcca cattcggtcg aaatatcact gattaacagg
    58741 cggctatgct ggagaagata ttgcgcatga cacactctga cctgccgcag atattgattg
    58801 atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa cgcgctcact gcacgatgcc
    58861 tcatcacaaa atttatccag cgcaaaggga cttttcaggc tagccgccag ccgggtaatc
    58921 agcttatcca gcaacgtttc gctggatgtt ggcggcaacg aatcactggt gtaacgatgg
    58981 cgattcagca acatcaccaa ctgcccgaac agcaactcag ccatttcgtt agcaaacggc
    59041 acatgctgac tactttcatg ctcaagctga ccgataacct gccgcgcctg cgccatcccc
    59101 acgctaccta agcgccagtg tggttgccct gcgctggcgc taaatcccgg aatcgctccc
    59161 tgccagtcaa gattcagctt cagccgctcc gggcaataaa taatattctg caaaaccaga
    59221 tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa tataaaagag atcgccacgg
    59281 gtaatgcgat aaggacgatc gttgagtaca tgcagaccat taccgcgcca gacaatcacc
    59341 agctcacaaa aatcatgtgt atgttcggca aagacatctt gcggataacg gtcagccaca
    59401 gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga gaagttttaa ctgatgcgcc
    59461 accgtggcta cctcggccag agaacgaagt tgattattcg caatatggcg tacaaatacg
    59521 ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc tggcgaatat cacgcggtga
    59581 ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta ctgtcgctga atccacagcg
    59641 ataggcgatg tcagtgacgc tggcctcgct gtggcgtagc agatgtcagg ctttcatcag
    59701 tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt tgctgcttaa gctgccgatg
    59761 tagcgtacgc agtgaaagag aaaattgatc cgccacggca tcccaattca cctcatcggc
    59821 aaaatggtcc tccagccagg ccagaagcaa gttgagacgt gatgcgctgt tttccaggtt
    59881 ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc ataaacaaga tctcgcgact
    59941 ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc atctgtgcaa ccagctgtcg
    60001 cacctgctgc aatacgctgt ggttaacgcg ccagtgagac ggatactgcc catccagctc
    60061 ttgtggcagc aactgattca gcccggcgag aaactgaaat cgatccggcg agcgatacag
    60121 cacattggtc agacacagat tatcggtatg ttcatacaga tgccgatcat gatcgcgtac
    60181 gaaacagacc gtgccaccgg tgatggtata gggctgccca ttaaacacat gaatacccgt
    60241 gccatgttcg acaatcacaa tttcatgaaa atcatgatga tgttcaggaa aatccgcctg
    60301 cgggagccgg ggttctatcg ccacggacgc gttacaagac ggaaaaaaat ccacactatg
    60361 taatacggtc atactggcct cctgatgtcg tcaacacggc gaaatagtaa tcacgaggtc
    60421 aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa aacgtcgatt tttcaagata
    60481 cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac cacaattcag caaattgtga
    60541 acatcatcac gttcatcttt ccctggttgc caatggccca ttttcctgtc agtaacgaga
    60601 aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga aattcagcag gatcacatta
    60661 tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc cagtgggcgc gtgatgctgg
    60721 cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga aatccatcgt tttaacaatg
    60781 ggctgcatag ccaaaacggt tatgtcacct gggatgtgga tagcctggaa agtgccattc
    60841 gccttggatt aaacaaggtg tgcgaggaag ggattcgtat cgatagcatt gggattgata
    60901 cctggggcgt ggactttgtg ctgctcgacc aacagggtca gcgtgtgggc ctgcccgttg
    60961 cttatcgcga tagccgcacc aatggcctga tgccgcaggc acaacagcaa ctcggcaaac
    61021 gcgatattta tcagcgtagc ggcatccagt ttctaccctt caatacgctt tatcagttgc
    61081 gtgcgctgac ggagcaacaa cctgaactta ttccacacat tgctcacgct ctgctgatgc
    61141 cggattactt cagttatcgc ctgaccggca agatgaactg ggaatatacc aatgccacca
    61201 ccacacaact ggtcaatatc aatagcgacg actgggacga gtcgctactg gcgtggagcg
    61261 gggccaacaa agcctggttt ggtcgcccga cgcatccggg taatgtcatt gggcactgga
    61321 tttgcccgca gggtaatgag attccggtgg tcgccgttgc cagccatgat accgccagcg
    61381 cggttatcgc ctcgccgtta aacggttcac gcgccgctta tctctcttct ggcacctggt
    61441 cattgatggg cttcgaaagc cagacgccat ttaccaatga cacggcgctg gcagccaaca
    61501 tcaccaatga aggcggggcg gaaggtcgct atcgggtgct gaaaaatatt atgggcttat
    61561 ggctgcttca gcgagtgctt caggaacggc aaatcaacga tctcccggcg cttatcgccg
    61621 cgacacaggc acttccggcc tgccgcttca tcatcaatcc caatgacgat cgctttatta
    61681 atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg ggaaacggcg caaccgatcc
    61741 cgggaagtga tgctgaactg gcgcgctgta ttttcgacag tctggcgctg ctgtatgccg
    61801 atgtgttgca tgagctggcg cagctgcgcg gtgaagattt ctcgcaactg catattgtcg
    61861 gcggcggctg ccagaacacg ctgctcaacc agctatgcgc cgatgcctgc ggtattcggg
    61921 tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat cggcatcgag ttaattacgc
    61981 tggatgaact caacaatgtg gatgatttcc gtcaggtcgt cagcaccacc gcgaatctga
    62041 ccacctttac ccctaatcct gacagtgaaa ttgcccacta tgtggcgcag attcactcta
    62101 cacgacagac aaaggagctt tgcgcatgac cactcaactg gaacaggcct gggagctggc
    62161 gaaacagcgt ttcgcggcgg tgaggattga tgtcgaggag gcgctgcgcc aacttgatcg
    62221 tttacccgtt tcaatgcact gctggcaggg cgatgatgtt tccggttttg aaaacccgga
    62281 aggttcgctg accgggggga ttcaggccac aggcaattat ccgggcaaag cgcgtaatgc
    62341 cagtgagcta cgtgccgatc tggaacaggc tatgcggctg attccggggc cgaaacggct
    62401 taatttacat gccatctatc tggaatcaga tacgccagtc tcgcgcgacc agatcaaacc
    62461 agagcacttc aaaaactggg ttgaatgggc gaaagccaat cagctcggtc tggattttaa
    62521 cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt acgctctcgc atcccgacga
    62581 cagcattcgc cagttctgga ttgatcactg caaagccagc cgtcgcgttt cggcctattt
    62641 tggcgagcaa ctcggcacac catcggtgat gaacatctgg atcccggatg gtatgaaaga
    62701 tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg gcagcactgg atgaggtgat
    62761 cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt gagagcaaat tgtttggcat
    62821 tggcgcagag agctacacgg ttggctccaa tgagttttac atggggtatg ccaccagccg
    62881 ccagactgcg ctgtgcctgg acgccgggca cttccacccg actgaagtga tttccgacaa
    62941 gatttccgcc gccatgctgt atgtgccgca gttgctgctg cacgtcagcc gtccggttcg
    63001 ctgggacagc gatcacgtag tgctgctgga tgatgaaacc caggcaattg ccagtgagat
    63061 tgtgcgtcac gatctgtttg accgggtgca tatcggcctt gacttcttcg atgcctctat
    63121 caaccgcatt gccgcgtggg tcattggtac acgcaatatg aaaaaagccc tgctgcgtgc
    63181 gttgctggaa cctaccgctg agctgcgcaa gctggaagcg gcgggcgatt acactgcgcg
    63241 tctggcactg ctggaagagc agaaatcgtt gccgtggcag gcggtctggg aaatgtattg
    63301 ccaacgtcac gatacgccag caggtagcga atggctggag agcgtgcggg cttatgagaa
    63361 agcgattttg agccagcgtg ggtaaacact gccggatgcg gcacgagcgc cttatccggc
    63421 ctacgggtcg aatgtccatg cgggctgtca ccctcacccc ggccctctcc ctgaaagggc
    63481 gagggagaaa aacatagcct cgtgcggcaa cggagctgaa caccgctccg gacagtcccc
    63541 tcgcccctac ggggagaggg ttagggtgag gggaacaagc cgcaccatgt ctaaacaagg
    63601 caacctaatt tacaggaaca cagaacatgc aaaacattac ccagtcctgg tttgtccagg
    63661 gaatgatcaa agccaccacc gatgcctggc tgaaaggctg ggacgagcgc aacggcggca
    63721 acctgacgct gcgcctggat gacgccgata tcgcgcttta tcacgacaac ttccacccac
    63781 aaccgcgcta tattccgctc agccaaccca tgcctttact ggcaaatacg ccgtttattg
    63841 tcaccggctc cggcaaattc ttccgtaacg tccagctcga tcctgcagct aacttaggcg
    63901 tcgtaaaagt cgacagcgat ggcgcgggct accatattct ctgggggtta accaacgaag
    63961 ccgttccaac ttctgaactt ccggctcact ttctctccca ctgcgagcgc attaaagcca
    64021 ccaacggtaa agatcgggtg atcatgcact gccacgccac caacctgatc gccctcacct
    64081 atgtactgga aaacgacacc gcggtcttta ctcgccaact gtgggaaggc agcaccgaat
    64141 gtctggcagt attcccggat ggcgttggca ttctgccgtg gatggtgccc ggcacggacg
    64201 aaatcggcca ggcgaccgca caagagatgc aaaaacattc gctggtgttg tggcccttcc
    64261 acggtgtgtt cggttccgga ccgacgctgg atgaagcctt tggtttaatc gacaccgcag
    64321 aaaaatcagc acaagtatta gtgaaggttt attcgatggg cggcatgaaa cagaccatca
    64381 gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt tacaccacta gccagtgcgc
    64441 tggcgctgta aggagcaaac atgatccgca aagcctttgt catgcaggta aaccccgacg
    64501 cccacgaaga gtatcagcgt cggcataatc ccatctggcc ggaactggaa gcagtgttga
    64561 aatctcacgg tgtgcataac tacgccattt acctcgacaa agcgcgtaat ctgctgtttg
    64621 ccatggtaga gattgaatct gaagaacggt ggaatgcggt agccagtact gatgtttgcc
    64681 aacgttggtg gaaatatatg accgatgtta tgcccgctaa cccagataac agcccggtga
    64741 gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc caatgtgccg gagatgcctc
    64801 cggcacaata ttttaaattt cgatttgaaa aattaaacat attacatctc ccggtaagtt
    64861 atatttccct gatacattgt gagtaaatca caaaaataat gaataaccca ttaatgattc
    64921 atgtggttta tttaaataac cattatgtgc attactccgc aaatctgacc tttcactctg
    64981 tctcaaattg tcattgttta ccgacatatc gcctctcatc acttaatcgc tccaccatta
    65041 caattgatta aaatatattt tatggagtcc gattaatggc agctcttact gcaagctgta
    65101 ttgacctgaa tattcagggc aatggcgctt attccgttct gaa
//
LOCUS       DADMEQ010000010        60679 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.010, whole genome shotgun sequence.
ACCESSION   DADMEQ010000010 DADMEQ010000000
VERSION     DADMEQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.010"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..320
                     /locus_tag="JNZ05_000646"
     CDS             <1..320
                     /locus_tag="JNZ05_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8563987.1"
                     /translation="VGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDS
                     NSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQV
                     TYP"
     gene            433..1227
                     /gene="panB"
                     /locus_tag="JNZ05_000647"
     CDS             433..1227
                     /gene="panB"
                     /locus_tag="JNZ05_000647"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAY8563988.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1239..2090
                     /gene="panC"
                     /locus_tag="JNZ05_000648"
     CDS             1239..2090
                     /gene="panC"
                     /locus_tag="JNZ05_000648"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAY8563989.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSTLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVGLA"
     gene            complement(2164..3066)
                     /locus_tag="JNZ05_000649"
     CDS             complement(2164..3066)
                     /locus_tag="JNZ05_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY8563990.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIPQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3340..3720
                     /gene="panD"
                     /locus_tag="JNZ05_000650"
     CDS             3340..3720
                     /gene="panD"
                     /locus_tag="JNZ05_000650"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAY8563991.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3724..4953)
                     /locus_tag="JNZ05_000651"
                     /pseudo
     CDS             complement(3724..4953)
                     /locus_tag="JNZ05_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(5017..5457)
                     /locus_tag="JNZ05_000652"
     CDS             complement(5017..5457)
                     /locus_tag="JNZ05_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY8563992.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5562..6332)
                     /locus_tag="JNZ05_000653"
     CDS             complement(5562..6332)
                     /locus_tag="JNZ05_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8563993.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6329..7255)
                     /locus_tag="JNZ05_000654"
     CDS             complement(6329..7255)
                     /locus_tag="JNZ05_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8563994.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCLNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRQVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7364..8026
                     /gene="can"
                     /locus_tag="JNZ05_000655"
     CDS             7364..8026
                     /gene="can"
                     /locus_tag="JNZ05_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAY8563995.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="JNZ05_000656"
     CDS             complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="JNZ05_000656"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAY8563996.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8809..11199
                     /locus_tag="JNZ05_000657"
     CDS             8809..11199
                     /locus_tag="JNZ05_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HAY8563997.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11246..12795)
                     /gene="cueO"
                     /locus_tag="JNZ05_000658"
                     /pseudo
     CDS             complement(11246..12795)
                     /gene="cueO"
                     /locus_tag="JNZ05_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
     gene            12961..13308
                     /locus_tag="JNZ05_000659"
     CDS             12961..13308
                     /locus_tag="JNZ05_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HAY8563998.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13414..14280
                     /gene="speE"
                     /locus_tag="JNZ05_000660"
     CDS             13414..14280
                     /gene="speE"
                     /locus_tag="JNZ05_000660"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HAY8563999.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQQS"
     gene            14296..15090
                     /gene="speD"
                     /locus_tag="JNZ05_000661"
     CDS             14296..15090
                     /gene="speD"
                     /locus_tag="JNZ05_000661"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HAY8564000.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15128..15490)
                     /locus_tag="JNZ05_000662"
     CDS             complement(15128..15490)
                     /locus_tag="JNZ05_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HAY8564001.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="JNZ05_000663"
     CDS             complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="JNZ05_000663"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HAY8564002.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIINRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18618..20471
                     /locus_tag="JNZ05_000664"
     CDS             18618..20471
                     /locus_tag="JNZ05_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HAY8564003.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVIPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDSNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSTVRERLSERGARRLER"
     gene            complement(20542..21978)
                     /gene="lpdA"
                     /locus_tag="JNZ05_000665"
     CDS             complement(20542..21978)
                     /gene="lpdA"
                     /locus_tag="JNZ05_000665"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAY8564004.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPNPNPKAKKK"
     gene            complement(22303..24195)
                     /gene="aceF"
                     /locus_tag="JNZ05_000666"
     CDS             complement(22303..24195)
                     /gene="aceF"
                     /locus_tag="JNZ05_000666"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAY8564005.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24210..26873)
                     /gene="aceE"
                     /locus_tag="JNZ05_000667"
     CDS             complement(24210..26873)
                     /gene="aceE"
                     /locus_tag="JNZ05_000667"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HAY8564006.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27034..27798)
                     /gene="pdhR"
                     /locus_tag="JNZ05_000668"
     CDS             complement(27034..27798)
                     /gene="pdhR"
                     /locus_tag="JNZ05_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HAY8564007.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28339..29712
                     /gene="aroP"
                     /locus_tag="JNZ05_000669"
     CDS             28339..29712
                     /gene="aroP"
                     /locus_tag="JNZ05_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HAY8564008.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29755..30609)
                     /gene="ampE"
                     /locus_tag="JNZ05_000670"
     CDS             complement(29755..30609)
                     /gene="ampE"
                     /locus_tag="JNZ05_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HAY8564009.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30606..31157)
                     /gene="ampD"
                     /locus_tag="JNZ05_000671"
     CDS             complement(30606..31157)
                     /gene="ampD"
                     /locus_tag="JNZ05_000671"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HAY8564010.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31245..32138
                     /gene="nadC"
                     /locus_tag="JNZ05_000672"
     CDS             31245..32138
                     /gene="nadC"
                     /locus_tag="JNZ05_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HAY8564011.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32341..32781
                     /gene="ppdD"
                     /locus_tag="JNZ05_000673"
     CDS             32341..32781
                     /gene="ppdD"
                     /locus_tag="JNZ05_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HAY8564012.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32791..34176
                     /gene="gspE"
                     /locus_tag="JNZ05_000674"
     CDS             32791..34176
                     /gene="gspE"
                     /locus_tag="JNZ05_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HAY8564013.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNTVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDTVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPAIRQLISANTEVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGK"
     gene            34166..35368
                     /gene="hofC"
                     /locus_tag="JNZ05_000675"
     CDS             34166..35368
                     /gene="hofC"
                     /locus_tag="JNZ05_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HAY8564014.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALHYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35403..36446)
                     /gene="guaC"
                     /locus_tag="JNZ05_000676"
     CDS             complement(35403..36446)
                     /gene="guaC"
                     /locus_tag="JNZ05_000676"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAY8564015.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            36671..37291
                     /gene="coaE"
                     /locus_tag="JNZ05_000677"
     CDS             36671..37291
                     /gene="coaE"
                     /locus_tag="JNZ05_000677"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAY8564016.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37291..38034
                     /gene="zapD"
                     /locus_tag="JNZ05_000678"
     CDS             37291..38034
                     /gene="zapD"
                     /locus_tag="JNZ05_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HAY8564017.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38044..38241
                     /gene="yacG"
                     /locus_tag="JNZ05_000679"
     CDS             38044..38241
                     /gene="yacG"
                     /locus_tag="JNZ05_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HAY8564018.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38341..38730)
                     /gene="mutT"
                     /locus_tag="JNZ05_000680"
     CDS             complement(38341..38730)
                     /gene="mutT"
                     /locus_tag="JNZ05_000680"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HAY8564019.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38790..41495)
                     /gene="secA"
                     /locus_tag="JNZ05_000681"
     CDS             complement(38790..41495)
                     /gene="secA"
                     /locus_tag="JNZ05_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAY8564020.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41557..42069)
                     /gene="secM"
                     /locus_tag="JNZ05_000682"
     CDS             complement(41557..42069)
                     /gene="secM"
                     /locus_tag="JNZ05_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HAY8564021.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42300..43217)
                     /gene="lpxC"
                     /locus_tag="JNZ05_000683"
     CDS             complement(42300..43217)
                     /gene="lpxC"
                     /locus_tag="JNZ05_000683"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HAY8564022.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVCVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43318..44469)
                     /gene="ftsZ"
                     /locus_tag="JNZ05_000684"
     CDS             complement(43318..44469)
                     /gene="ftsZ"
                     /locus_tag="JNZ05_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAY8564023.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44530..45792)
                     /gene="ftsA"
                     /locus_tag="JNZ05_000685"
     CDS             complement(44530..45792)
                     /gene="ftsA"
                     /locus_tag="JNZ05_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAY8564024.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45789..46619)
                     /gene="ftsQ"
                     /locus_tag="JNZ05_000686"
     CDS             complement(45789..46619)
                     /gene="ftsQ"
                     /locus_tag="JNZ05_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HAY8564025.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46621..47541)
                     /locus_tag="JNZ05_000687"
     CDS             complement(46621..47541)
                     /locus_tag="JNZ05_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAY8564026.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLKASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47534..49009)
                     /gene="murC"
                     /locus_tag="JNZ05_000688"
     CDS             complement(47534..49009)
                     /gene="murC"
                     /locus_tag="JNZ05_000688"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAY8564027.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49063..50130)
                     /gene="murG"
                     /locus_tag="JNZ05_000689"
     CDS             complement(49063..50130)
                     /gene="murG"
                     /locus_tag="JNZ05_000689"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAY8564028.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50127..51371)
                     /gene="ftsW"
                     /locus_tag="JNZ05_000690"
     CDS             complement(50127..51371)
                     /gene="ftsW"
                     /locus_tag="JNZ05_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HAY8564029.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51371..52687)
                     /gene="murD"
                     /locus_tag="JNZ05_000691"
     CDS             complement(51371..52687)
                     /gene="murD"
                     /locus_tag="JNZ05_000691"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAY8564030.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPSL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52690..53772)
                     /gene="mraY"
                     /locus_tag="JNZ05_000692"
     CDS             complement(52690..53772)
                     /gene="mraY"
                     /locus_tag="JNZ05_000692"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAY8564031.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53766..55124)
                     /gene="murF"
                     /locus_tag="JNZ05_000693"
     CDS             complement(53766..55124)
                     /gene="murF"
                     /locus_tag="JNZ05_000693"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAY8564032.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55121..56608)
                     /gene="murE"
                     /locus_tag="JNZ05_000694"
     CDS             complement(55121..56608)
                     /gene="murE"
                     /locus_tag="JNZ05_000694"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAY8564033.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56595..58361)
                     /locus_tag="JNZ05_000695"
     CDS             complement(56595..58361)
                     /locus_tag="JNZ05_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HAY8564034.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58377..58742)
                     /gene="ftsL"
                     /locus_tag="JNZ05_000696"
     CDS             complement(58377..58742)
                     /gene="ftsL"
                     /locus_tag="JNZ05_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAY8564035.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58739..59680)
                     /gene="rsmH"
                     /locus_tag="JNZ05_000697"
     CDS             complement(58739..59680)
                     /gene="rsmH"
                     /locus_tag="JNZ05_000697"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAY8564036.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59682..60140)
                     /gene="mraZ"
                     /locus_tag="JNZ05_000698"
     CDS             complement(59682..60140)
                     /gene="mraZ"
                     /locus_tag="JNZ05_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAY8564037.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCAIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14533 a        16806 c        14923 g        14417 t
ORIGIN      
        1 aggttggtac tgaaaacggg caactccttg gtaatactct cacaggtaat gacgcagcaa
       61 aaggagtcgg tgtactcata gaaggtttag caactagtaa aaatcctcta atgacattga
      121 aacctaatga ttcaaattct gtttataaag attacgaccc gagaggcaaa gacgatacaa
      181 caggaggagt ttacccggat caagatacag gtataacata ccctctccat ttccaggcca
      241 cgctacaaca ggatggaact ataccaatag aagctggtga gtttaaagcc accagtactt
      301 tccaggtaac ctacccttaa taagtccacc gcaccgccat catctggcgg tgcggtaatt
      361 gataaatcat tgcccccacc aatgtgactg ataaaatgcc aggcaacacg acatcattta
      421 tcaggatacg ttatgaaacc gaccaccatt gcttcacttc agaaatataa gcaggataaa
      481 aagcgtttcg cgaccatcac cgcttacgac tacagcttcg cgaaactatt tgctgatgaa
      541 gggcttaacg tcatgctggt aggcgactcg ctgggcatga ccgttcaggg ccacgattca
      601 actctgccag taaccgttgc tgatatcgcc taccacactg ccgccgtacg tcgcggcgca
      661 ccaaactgcc tgctattggc tgacctgccg tttatggcgt atgccacgcc ggaacaagcc
      721 tttgaaaacg ccgcaacggt tatgcgtgcc ggtgccaata tggtcaaaat tgagggcggt
      781 gagtggctgg tcgaaaccgt aaaaatgctg accgaacgtg ccgttcctgt atgtggtcac
      841 ttaggtttaa ccccacagtc agtgaatatt ttcggtggct acaaagtaca ggggcgcggc
      901 aatgaagcga gcgatcgact actcagcgat gcattagcct tagaagccgc gggggcacag
      961 ctgctggtgc tggaatgcgt gccggttgag ctggcaaaac gtattaccga agcactggcg
     1021 atcccggtta ttggtattgg tgcaggcaac gtcactgacg ggcaaatcct cgtgatgcac
     1081 gacgcctttg gtattaccgg cggtcacatt cctaaattcg ctaaaaattt cctcgccgaa
     1141 acgggcgaca tccgcgcggc tgtgcggcag tatatggctg aagtggagtc cggcgtttat
     1201 ccgggcgaag aacacagttt ccattaagga gtcacgttgt gttaattatc gaaaccctgc
     1261 cgctgctgcg tcagcaaatt cgccgcctgc gtatggaagg caagcgcgtg gcgctggtgc
     1321 ctactatggg taatctgcac aatggccata tgaagctggt cgacgaagcc aaagcccgcg
     1381 ccgatgtggt cgtcgtcagt attttcgtta acccgatgca gttcgaccgc ccggaagatc
     1441 tggcacgtta tccacggacc ttgcaggagg actgcgagaa gctaaacaaa cgtaaagtgg
     1501 atttagtttt cgccccttcg gtaaaagaga tctacccgaa cggtactgaa acccacactt
     1561 acgttgacgt tcctggcctt tcaaccatgc tggaaggtgc cagccgtccg ggacattttc
     1621 gcggcgtttc gactattgtc agcacgctgt tcaacctggt ccagccggac atcgcctgct
     1681 tcggtgaaaa agattttcag caactggcgt tgatccgcaa aatggttgcc gatatgggct
     1741 tcgatattga gattgtcggt gtgccaatta tgcgcgccaa agacggtctg gcgctaagtt
     1801 cccgtaacgg ttatctgacg gcggaacaac gcaaaattgc gcctggtctg tacaaagttt
     1861 taagttcgat tgctgacaaa ttgcaggctg gggaacggga tctcgatgaa attattacca
     1921 ttgcggggca agaactgaat gaaaaaggct tccgcgccga tgatattcag attcgcgatg
     1981 ccgacacatt gctggaagtt tctgaaacca gcaaacgggc agtaattctg gtagccgcct
     2041 ggcttggcga tgctcgcctg atcgacaaca aaatggtcgg gctggcgtaa tacttaactg
     2101 gcgctacggc tgatggcgcc agttattaat ttaccccacg tcatctgtca taacacaggt
     2161 aacttaaata aggttaatta ccttatcaat ctcagcaaga ggtaaatttg ccatctctgc
     2221 aacgtcttcc cgagacattc ctttacttag aagacgcagg gcgaattctt gcctttcctc
     2281 ctgtcttccc tgcggaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2341 catcatagac ttccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2401 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2461 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2521 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2581 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gcgccagctc
     2641 cggcgagtaa aacatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     2701 ataaaacagt atcggcacca ccagcggcag cttatcgtgg ccagcctcca gatgccggtg
     2761 catggcagca atagaataac gcatcatgcg aaaggccatt ttcttatccg gcttgctttg
     2821 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     2881 gtccgtgctg tgacctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     2941 gttgagatca caaagttcgc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3001 cgtctccgca tgcattaaaa attgtttaaa taccgcgtca tgcggtgtgg tactcggtgc
     3061 atccatcttc cctgtcttcc gtgtggcctt gcgtgaaggc agtatccagt caaggcgtgc
     3121 tatcgtcagc cacctcttta ttgtttaccg agcagcattc ataaaaatat caattcgtta
     3181 caggcgttac atggcacgct tcggcgcggt acgcaaactg ctgacgcaaa tgccatcaga
     3241 tgagtagaca ctcatcaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcac
     3301 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3361 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3421 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat cgatatctgg
     3481 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3541 attatttctg ttaacggtgc ggcggcccac tgcgccagcg tcggcgatat tgtcatcatc
     3601 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa tgtcgcctat
     3661 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     3721 ttgttatccc tgcggctggt tactcaccag ccgcgacatc ttctccagcg aatccgttct
     3781 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     3841 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     3901 gccgccaaac ggatacgaaa gataccagac atgtggatta aattgcgcca gagcgcggcg
     3961 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4021 ataaccatct acccgatgca aaaaatgggt atgtgactgg aaatcaaaaa catcgcgaat
     4081 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggctccact tctgcgggtg
     4141 acgtttgatg cgtgaggtaa caataaacac cgtcgccttc atgccatatt gtttcaacac
     4201 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4261 cgcagggaga ttgatcgtat tcttcacgta accttccagc tgagccatgc tcagtgtcgc
     4321 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4381 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4441 cggcaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4501 acggatctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4561 caaattgtcc gccagtaccc caaatggcgc actgcccaca ctcggcgcgt tgtagaccgg
     4621 cgtatctttc taggtaatta agttctgatt actcagcggc ttgttaaggt cacccaaacc
     4681 gtcttcaact ttttgtcgcc cctgaactgg ctcgagatgc cctttatcga taaacccttt
     4741 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     4801 tccggcccga atattcccca cggtcaccat cttgtcacca atttgcgccc agaccgcagc
     4861 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     4921 catcagcagg agaataacag cttgtttata catgaaaaaa taaccgaggg tgaaacaacg
     4981 cttttattgt agcaaaagcg ccatcagaat cgcgggttaa tactcataca aattatgttt
     5041 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5101 cagttcgaca atacactcac gaaactctga actggtcatg gtttcacggc aagccatcat
     5161 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5221 taataatgaa gccacgcgat acggtggcgc tccggctata tcggttaaga agatgacacc
     5281 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5341 taatccgcgc cagaaattca cggcccggca ctgaagaagt gccccatgtt ttttctccag
     5401 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5461 cttcctcctt agccgaaaat cagcctaccc tgaccattgt tgataaaggg tgatagccgt
     5521 caaaactcct ctcccccgaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5581 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     5641 ccaaaggtag tgaccagcgg cacatcattt ataccgagga agccgtagcg gaaaccactg
     5701 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     5761 agtgagtaaa agaccccgcc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     5821 ctgatgtcat cgaacgtttt ggcaaacacg ccgttcagca aacccgcaag ggagaatagc
     5881 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     5941 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6001 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6061 ttacgctgga acttggcacc aaaaaatgat gacgcgacgt tagcgtaggc gttggtgatc
     6121 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6181 ccaatacgcg aacctatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6241 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6301 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6361 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6421 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgtagc acttcaactt
     6481 ccagcgtcgc agtatcgacc tgtcgatact gatagccatc gagcttcggt aacggacttt
     6541 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6601 aggtattttc caccagctca ccatgttgaa taatgccgat attgaggcac agcatttctg
     6661 cttcttccag gtagtgtgtg gtaagaatga tggtggtgcc tttgtcgttt aaatccttca
     6721 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     6781 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     6841 cagataacat acgcgcacgt tcgttgcgtt ttccccataa atcgagctgc ttaagatatt
     6901 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     6961 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7021 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7081 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7141 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7201 gaacgccgcc tggatagttt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7261 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7321 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7381 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7441 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7501 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7561 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7621 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     7681 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     7741 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     7801 gtgtgaactg aacgtcatgg aacaggtgta taacctgggc cactccacca ttatgcaatc
     7861 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     7921 cttgctgcgt gatctggatg ttaccgccac caaccgcgaa acccttgagc aacgttaccg
     7981 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8041 atgcaacaca tccggcaact tcacacttac tcgtccagca gaatcacttt gccaatatac
     8101 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8161 atcgagaaac cgataaattc aaccgggacg ttcacttcac gacgggacgg tttatccagc
     8221 agcgtacaaa tcgccaacga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8281 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8341 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8401 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8461 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8521 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8581 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     8641 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     8701 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     8761 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     8821 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     8881 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     8941 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg
     9001 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9061 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9121 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9181 gtggtcgcac tgctgattag cggtggtatc ctgacctggg ccggatttaa cgatccgcag
     9241 gagatcaacg gcaccttaag cgccgatgcc acacctgccg aagctatctc ccccgttgcc
     9301 gatcaggact ggcctgcgta tggccgcaat caggaaggtc aacgcttttc accactgaag
     9361 caaattcacg ccgataacgt ccacaagctg aaagaagcct gggtgttccg tactggcgat
     9421 gtgaagcagc cgaacgatcc gggtgaaatc accaatgaag tgacgccgat taaagtgggc
     9481 gatacccttt acctgtgtac cgctcaccag cgcctgtttg cgcttgatgc cgccagcggc
     9541 aaagagaaat ggcattacga tcctgagctg aaaaccaacg agtctttcca gcacgtaacc
     9601 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
     9661 gattgcccgc gtcgtatcat tcttccagtg aatgatggtc gcctgattgc gattaacgct
     9721 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
     9781 atgccagaca ccaaaccggg tctgtatgag ccgacttctc cgccaattat caccgataaa
     9841 accatcgtga tggccggttc agtcaccgat aacttctcaa cccgcgaaac gtctggcgtg
     9901 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc gggcgcgaaa
     9961 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcacc aaactcgtgg
    10021 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10081 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10141 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10201 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10261 gtgccgatta tttacgctcc ggcgaaaacc ggtaacattt ttgtgctcga tcgtcgtaat
    10321 ggcgaactgg tggttccggc cccggaaaaa ccggttcccc aaggtgctgc caaaggcgat
    10381 tacgttaccc ctactcaacc attctcggaa ctgagcttcc gtccgacgaa agatttgagc
    10441 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10501 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggtacgct ggtcttcccg
    10561 ggtaacctgg ggatgttcga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10621 attgccaacc caatggcact gccgtttgtt tcgaaactgc tcccgcgtgg tcctggcaac
    10681 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    10741 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccatttgg tctgccatgt
    10801 aaacagccag catggggtta tatctcggcg ctggatctga aaactaatga agtggtgtgg
    10861 aagaaacgta ttggtacgcc gcaggacagt atgccgttcc cgatgccggt tccggtgccg
    10921 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    10981 atcgccgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11041 tggcagggtc gtttaccggc gggtggtcag gcaacaccga tgacctatga agtgaatggc
    11101 aagcagtacg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11161 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagaaatcc
    11221 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11281 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11341 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11401 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcgtg ccgtgaatat
    11461 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11521 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11581 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccatgc cccatatggc
    11641 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    11701 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    11761 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    11821 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggtgcaatcg
    11881 ccatccccat ctggctgacc ggcagcgtca ccaggtcaaa gggtttgttg tcgttaacct
    11941 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12001 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12061 aattgagcga gcgggcatta cagccattga gcaaacgcag gcgcagccaa ccacgcgggg
    12121 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12181 cggcggtcat cacatccagt tgataatcaa tctgcccgtc ggcgctaaat ttcttatcct
    12241 gaacgatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12301 cgtcatcttc aatcaccacc agaccagcca gccccatcgc cacctgtcgc cggtcttgcc
    12361 atgttgatgt ggatggaacc agcaggtagc ggcaggttga tcaacgttca acgtcaccga
    12421 gcgcttgcca cctggcggaa taattccctg tgggccgccg tcgacgtcac ccggtacttc
    12481 cagcccgtgc cagtgcaacg tcgtctcttc cgccagttgg ttgtagatat caaccgttac
    12541 cgctttgccg cgctgtaatt tcaccgccgg ccccagcaga ttgccgttat agccccaggt
    12601 agttgcagtt ttctcgccaa aggtggactg acctgcgcca atagttaact gaatgcgatt
    12661 acgggcatcg gtcgtgagca aatcagggat cggtaacgtt gggcgttccg ccgcaaagac
    12721 tgcacggctc cacagtggca aggctgaagc cacacccagt gcgacggaat atttcaagaa
    12781 atcacgacgt tgcatagtta tttccttatt cttaagcagg cgaaacaaaa tcaaacgttg
    12841 agcatagtcc ttccccttac gggaaggtca agccgcagac acaatttaat cgacaataat
    12901 ggtcgtcgcc tcgctcgcag tgtgctaacg tttatcttct ttaagcccct ggtagaagca
    12961 atgaagacgt ttttcagaac agtgttattc ggcagcctga tcgccgtttg cgcaaacagt
    13021 tacgcgctca gcgagtctga agccgaagat atggccgatt taacggcagt ttttgtcttt
    13081 ctgaagaacg attgtggtta ccagaactta cctaacgggc aaattcgtcg cgcactggtc
    13141 tttttcgccc agcaaaacca gtgggatctc agtaattacg acaccttcga catgaaagct
    13201 ctcggtgaag acagctaccg cgatctcagc ggcattggca ttcccgtcgc taaaaaatgc
    13261 aaagccctgg cccgcgattc cttaagcctg cttgcctacg tcaaataatc cctgatacct
    13321 ttttgtagaa atattgaccg tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt
    13381 ttttacgggt gttaacaaag gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa
    13441 acgctacacg accagtttgg gcagtacttt gcggtagata acgttctgta tcatgaaaag
    13501 accgatcacc aggatctgat catttttgag aacgctgcat ttggtcgcgt aatggcgctg
    13561 gatggcgtag tacaaaccac cgagcgcgac gagtttatct atcatgagat gatgacccat
    13621 gttccgctac tggcccacgg tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt
    13681 gccatgctgc gtgaagtaac ccgacataaa aacgttgagt caatcacgat ggtggaaatc
    13741 gatgcgggtg tcgtgtcgtt ctgccgtcag tatctgccca accataacgc cggtagctac
    13801 gacgatccgc gctttcaact agtgatcgac gatggcgtca atttcgttaa tcaaaccagc
    13861 cagacctttg atgtcattat ctccgactgc accgatccta tcggtcccgg cgaaagcctg
    13921 ttcacctcag cattttatga aggctgcaaa cgttgcctga atcctggcgg tatcttcgtc
    13981 gcacaaaacg gcgtctgctt tttacagcag gaagaagcca tcgacagcca tcgcaaactc
    14041 agccattact tcagcgacgt tggcttttat caggcggcga tcccgaccta ttacggcggt
    14101 atcatgactt ttgcatgggc gacagataac gacgccttac gccatctctc aaccgaaatt
    14161 attcaggcgc gttttctcgc ttctggcctg aaatgccgtt attacaatcc ggcaatccat
    14221 acggcagctt ttgccttacc tcagtatctg caagacgcac tggcttcaca gcagtcctaa
    14281 ggagaagata agaaattgaa aaaactgaaa ctgcatggct ttaataatct gaccaaaagt
    14341 ctgagttttt gtatttacga tatctgctac gccaaaactg ccgaagagcg cgacggttat
    14401 attgcttata tcgatgaact ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc
    14461 tgttccatta tcggggccaa tattcttaac atcgcccgcc aggattacga accacagggt
    14521 gccagcgtca ctattctggt gagtgaagaa ccggttgacc cgaaactcat cgacaaaaca
    14581 gaacaccccg gcccactgcc agaaacggtg gttgctcatc tcgataaaag tcatatttgc
    14641 gtacatacct acccggaaag tcatcctgaa ggcggtttat gtaccttccg cgccgatatt
    14701 gaagtctcta cctgcggcgt gatttctccg ctgaaggcgc tgaattacct gatccaccag
    14761 cttgagtccg atatcgtaac cattgattat cgcgtgcgcg gttttactcg cgacattaac
    14821 ggtatgaagc actttatcga ccatgagatt aattcgattc agaactttat gtctgacgat
    14881 atgaaggcgc tgtatgacat ggtggatgta aacgtctatc aggaaaatat cttccatacc
    14941 aagatgttgc ttaaagagtt cgaccttaag cactacatgt tccacaccaa accggaagat
    15001 ttaaccgaca gcgagcgcca ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt
    15061 tattacgggc gcaatatgcc agctgtttaa cggctctggc ggagctccca ggctccgcca
    15121 gatttattta cttctgctgc acgaaattgc ggtaagccgc cacgacctgc agaaaatcct
    15181 caacgccgca tagcgacagg ctttcttcgt cgtagtagtt catcccctct tccatttcat
    15241 cgccagcgaa ttccagttga ttggcgcgaa ccatcacctc ttcagcgtcc atccacaggg
    15301 tgtattcatg ccctgcccgt tgccaggacc gttcgctacc tttcagtgcg tgcgctgctt
    15361 gttccacttc atcaagcaag gccaggtttt ctttcacctc ttcattaaac cagtgcccga
    15421 ccacttcatg ccccatggac atacgcacct ttaccactcc ggtaatatcg cgcagaaatt
    15481 cgtaatccat agtgtgttcc tctgctcccg gcaatgcgcc gaaatcgcat cgcgccattg
    15541 atgtaattat cgcagcctta ccggagaaaa acagcggaac ggcgatggtt tagaaataaa
    15601 aaatgcccgg gaatctcatt caccgggcat tggtgtcgtt tatgcgcagc gcgtgcgctt
    15661 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    15721 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    15781 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    15841 gcagaagcca ggaagacatt cgcgccagta cccaaacggt tcgggaagtt acgggtagag
    15901 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagggaacag
    15961 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttccgtc
    16021 agttgcgcgg cgtccatacg ggttggcggt gccacccaca ggcgggtcgg caactgacct
    16081 ttgtgcgcat ccagcagttt accagcggca cggaagtgac cgatgttagt catgcaagaa
    16141 ccgataaata cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcatcc
    16201 ggatcgttcg gagcacacag gattggctct ttaatctccg ccagatcgat gtcgatcact
    16261 gccgcgtatt ccgcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16321 atgccctgaa tacggcgttc cagggtacga cgatcgccgt aaccttccgc gatcatccac
    16381 ttcagcagga cgatgttgga gctcaggtat tcgatgatcg gttctttgtt cagcttgata
    16441 gtacaaccag cggcagaacg ctcggcggac gcatcggtta gctcaaaggc ctgctcaact
    16501 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16561 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcggaatggc gtgtaccaga
    16621 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    16681 atatccagcg gcattacgcc agttgcggca gcaaacgcca ccagaccaga acccgccggg
    16741 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    16801 agcagcatac ggttcagcca ggagtgaatg acgccgtcac ccggacgcag cgacacaccg
    16861 ccacggttaa taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    16921 tacgccgcgg tgtggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    16981 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc
    17041 attttcggtt cgcagtacgc gcccggacga atgcctttca cgccacaggc acggcctacc
    17101 attttctgcg ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17161 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17221 atcagcggaa tacggccgcc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17281 aaggtcgcca gcagttcgcc tgtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17341 tcaatcacgt cgcccatgtt caggttagag acgttgactt cgattggcag tgcacccgcg
    17401 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17461 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17521 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17581 tgttgcagag cttcaatctg tttgatcggg ccaacaacac ctggctggtc tggctcaata
    17641 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac
    17701 gcatccggtg ccggagaaag gtcatcggtg ttggtttcgc cagtgacttt aaagacggta
    17761 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    17821 tgcattacct gcttcgcata ttcgttgcct gctttcgctt tctcttctac gtcatagaag
    17881 ttatcgaaca tcagcagcgt gtgagaaagt gctttggcag caataggtgc cagtttggca
    17941 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt gcccagcagt
    18001 tcgatagctt tttccggagt cagcagaggg gatttagctt cgcctttagc gatagcagcc
    18061 aggaagcctg cttttacata ggcggcttca tcgacgcctg ggggaacacg gttggttaac
    18121 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagcgcg
    18181 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18241 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18301 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18361 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18421 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18481 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18541 cgcctcttta tactcggatt cacagcacct gcgggtggca gttcgccgac cattgcgatt
    18601 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcgctaa
    18661 tttgcagtgc cgggctttgt gctgcctctg ccgggctata tataaaaagc cgcacagtgg
    18721 aagcgcctgt agaaccgcaa tcgacacaac tggctgtgtc tgacgctgcc gctgttacgc
    18781 ttcctgcaac ggtttccgca cctcccgtaa tacccgcagt cgtcaaatcc gcattcagca
    18841 ctgcacaaat agatcaatgg gtcgcacccg tcgcgctgta tcccgacgcc ctactttcgc
    18901 aggtgctgat ggcatcaacc tatccgacaa acgttgctca agcagtgcaa tggtcgcacg
    18961 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19021 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19081 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19141 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19201 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19261 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19321 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19381 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19441 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19501 acgcactatt cagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19561 atgatgatta tcatcaccac gatggcggtc atcgtgacag taatggctgg caacacaacg
    19621 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    19681 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    19741 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    19801 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    19861 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    19921 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    19981 tgacccagca gcaaaaggac gccgctcgtc agcgttatca gtcggcctct cctgagcagc
    20041 gccaggcagt ccgcgagaaa atgcagacta acccgcagat ccagcagcga agggaggcag
    20101 cgcgccagcg tattcagtcc gcctcgcctg agcagcgcca ggcggttcgc gagaaaatgc
    20161 agactaaccc gcagaaccag cagcgaagag aggcagcgcg tgagcgtatt cagtctgcaa
    20221 ctcccgaaca gcgccaggtg tttaaggaaa aagtacagca gcgcccactg aaccaacagc
    20281 aacgtgataa cgcccgccag cgtatacaat cggcatcacc tgaacaacgt caggtttttc
    20341 gggagaaagt tcaggagagc cgcccacaac gtctaaacga cagtaaccat actgtcaggc
    20401 tgaataacga gcaacggtca acagtacgcg aacgtctctc tgaacgtgga gcaagaagac
    20461 tggaaaggta aattgcagac gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg
    20521 gatgttccgg caaacgaaaa attacttctt cttcgctttc gggttagggt tcgggttcgg
    20581 caggtcggta atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt
    20641 cgggtgcgcg tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag
    20701 cccgatttca cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacgcg
    20761 gtgagattct ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc
    20821 acgaccagaa gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc
    20881 ttctttctca gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat
    20941 aactttcgga tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc
    21001 gtgaacacct ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg
    21061 cggtacgttg gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac
    21121 gccagctttg cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac
    21181 ggcgtcgtaa cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc
    21241 gtcttctttc gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct
    21301 gatacgcttg gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa
    21361 catttcaacc acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc
    21421 gatgataccg ccacccatta ccagcaggcg ttctggcact tctttcagtt ccagcgcgtc
    21481 agtggagtcc cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga
    21541 acccgctgca atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac
    21601 ttccagggtg ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc
    21661 tttcgccata ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg
    21721 aatcttgtca atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt
    21781 ggcttcttcg ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt
    21841 caggcaaaca ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc
    21901 agcgcaacgg aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt
    21961 tttgatttca gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt
    22021 ctttcaggcc gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc
    22081 tcctggggat tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag
    22141 taatacccta accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa
    22201 tttttaaaca acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg
    22261 agattaccag aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg
    22321 aatgtcagac agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat
    22381 cacgcggtgg tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc
    22441 attccacacc ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt
    22501 cacaatcggc gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc
    22561 gccctgcatt tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag
    22621 ctcgcgagac agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag
    22681 accgttcggg gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg
    22741 accgtcttcc gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc
    22801 tttcatgatg aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc
    22861 gttctgctgt ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac
    22921 atgcgggatc atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc
    22981 cagttccact tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat
    23041 gccagggata ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt
    23101 cacgtaagcc tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt
    23161 cgccaggtta acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta
    23221 agcgtcgttt tcagcaaatt cagatttgcc ttccgctttc gcagccggag ctgctgccgg
    23281 agcttcagct tttgctgccg gagccggcgc tgccgcttcc tgtttcgcag gagctgccgc
    23341 aggcgctgcg ccttcaactt cgaagatcat aatcagcgaa ccagttttca ctttatcgcc
    23401 aacgttgact ttcagttcct tcacgacgcc tgcaaacgga gccggaactt ccatagaagc
    23461 tttatcgcct tctacggtaa tcagtgactg ttcagcggca actttgtcgc ccactttcac
    23521 catcacttcg gtcacttcaa cttcgtcacc gccgatatcc ggaacgttaa cttctttcac
    23581 gccagctgct ggtgcggggg tcgctgccgg agtcgcttcc tgtttagccg ccggagctgc
    23641 cgcgcctgct tcacccgcga cttcgaagac cataatcagc gagccggtag acactttgtc
    23701 acccacgttc actttgatct ctttcacggt acctgcaaac ggagccggaa cttccataga
    23761 agccttgtcg ccttctacgg tgatcagcga ctgttcagct tcaactttat cgccaacttt
    23821 caccaggatt tcggtcactt caacttcgtc gctgccgata tccggaacgt taacgtcttt
    23881 tgccgccgca gccgcgggtg ctgctgccgg agctgcttct ttcttctctt ctgcctgagc
    23941 aggtgcagcg tctgctgcac cgtcggcgga atcgaaaatc ataatcagtg cgccggtctg
    24001 ggttttatcg ccaacagaga ctttgatctc tttaacgata cccgcctgcg gagatggaac
    24061 ttccatagag gctttgtcgc cttctacggt gatcagcgac tgttcggctt caactttgtc
    24121 gcccactttg accaggatct cggtgatttc aacttcatca gccccgatgt ccggtacttt
    24181 gatttcgata gccattattc ttttacctct tacgccagac gcgggttaac tttatctgca
    24241 tcgatgttga atttggcgat tgcgtcagca accactttct tatcgatttc gccacgttta
    24301 gccagttcgc ccagcgccgc aaccacgaca taagaagcat caacttcgaa gtggtgacgc
    24361 aggttctcac ggctgtcgga acgaccgaag ccatcagtac ccagtacgcg atagtcgtca
    24421 gccggtacgt aagtacggac ctgctcagcg aacagtttca tatagtcggt agatgccact
    24481 gccggcgcgt cgttcatcac ctgagcgata tacggaacgc gcggagtttc cagcggatgc
    24541 agcatgttcc agcgttcaca atcctgacca tcacgcgcca gctcggtgaa ggaggtcacg
    24601 ctataaacgt cagaacctac gccgtaatct ttcgccagga tctctgccgc ttcacggacg
    24661 tgacgcagga tggaaccgga gcccagcagc tgaactttac ctttgctacc ttcaatggtt
    24721 tcgagtttgt agataccttt acggatacct tcctcagcac cttccggcat tgccggcatg
    24781 tggtagtttt cgttcagcgt agtgatgtag tagtaaacgt tctcttgttt ttcaccgtac
    24841 atacgctcca gaccgtcatg cataatgaca gcaacttcgt aagcgtaagc cgggtcgtaa
    24901 gagatacagt tcgggatagt cagcgactga atgtggctgt gaccatcttc gtgctgcaga
    24961 ccttcaccgt tcagggtggt acgaccggaa gtaccgccga tcaggaagcc acgcgcttgc
    25021 tggtctccag ccgcccagca cagatcgccg atacgctgga agccgaacat cgagtaatag
    25081 atgtagaatg ggatcatcgg cagattgttc gtgctgtaag aggtcgccgc tgccagccag
    25141 gaacaccctg cgcccagctc gttgatccct tcctgcagaa tctgaccttt ctcgtcttct
    25201 ttatagtaag caacctgctc gcggtcctgc ggggtgtact gctgaccgtt cgggctgtaa
    25261 ataccaatct gacggaacag accttccata ccgaaagtac gcgcttcgtc ggcgatgatc
    25321 ggtaccagac gatctttgat cgacttgttc ttcagcatca cgttcagagc acgaacaaaa
    25381 gcgatagtgg tagagagctc tttgctctgc tcttccagca gcgcgccgaa gtcttgcagg
    25441 ctcggcagct caagcttctc ggtgaagttc ggctgacggc ttggcagata accgtgcagt
    25501 ttctgacgct gagcgtgcag ataggtatgc tcttcagaac cttccgggaa ggtgatgtac
    25561 ggcagttttt cgatatctgc atcagacacc ggcacattga aacggtcgcg gatgtgacgc
    25621 acgccgtcca tgttcatttt cttaacctgg tgcgcgatgt ttttaccttc agccgcgtcg
    25681 cccatgccgt aacctttaat ggtatgagca aggattactg tcgctttgcc tttggtttcc
    25741 tgcgctttct tgaatgcagc gtagattttc ttcggatcgt gaccaccacg gttcagtgcc
    25801 cagatctgct cgtcagtcca gtctgcaacc agtgctgcgg tttcaggata tttaccgaag
    25861 aagtgttcac gaacgtacgc accatctttc gatttgaagg tctggtagtc gccgtcaacg
    25921 gtttcgttca tcagctggat cagtttaccg ctggtatctt tacgcagcag ttcatcccaa
    25981 cggctacccc acatcacttt gatcacgttc cagccagcac cttcgaagat gccttccagt
    26041 tcgttgatga tcttgccgtt accggtgacc gggccgtcaa gacgctgcag gttacagttg
    26101 ataacgaaaa ccaggttatc cagtttttca cgggtagcga tggtgatcgc acctttggat
    26161 tccggttcgt ccatttcacc gtcgccgagg aacgcgtaaa cggtctgttt agaggtatct
    26221 ttcaggccac ggtgttccag atatttcagg aatttagcct ggtaaatagc accaatcgga
    26281 cccagaccca tagatacggt cgggaactgc cagaattccg gcatcagttt cgggtgcgga
    26341 taggaagaga ggccattgcc gtgaacttcc tgacggaagt tatccagctg ctcctgagtc
    26401 agacgacctt ccaggaaagc acgggcgtaa acgcccgggg agatgtggcc ctggaagtaa
    26461 accaggtcgc cgccatcctg ctcgttgcgt gcacggaaga agtggttaaa gcacacatca
    26521 taaatggttg cggaagactg gaaggacgcc atgtggccgc ccagttcgag gtcttttttc
    26581 gacgcacgca gcaccgtcat gatggcgttc cagcggatag ctgaacgaat acggcgttcc
    26641 agttccagat tacccggata ctccggttgt tcttcaacgg ggatggtgtt gatgtagttg
    26701 ctgatacctg tgcctgcggc tacgtttaca ccgcctttgc gggcttcagc aagcagttgg
    26761 tcgatcagat actgagcacg ctcaacacct tcttcacgga tgaccgattc gatcgcctgg
    26821 agccagtcgc gagtttcgat cggatccacg tcatttggga aacgttctga catgggttat
    26881 tccttatcta tctaataacg ttgagttttc tggaacctgt cccattgaac tctcgccaga
    26941 aagctcaata agacaggttc tacgtttagt taccgcgctt ttatatgcgc ttgatttaca
    27001 acatcttctg gataattttt accagaaaaa tcactaattc tttcgttgct ccagacgacg
    27061 cagagaacgc tcacggcggc tctcttcacg gcttctgtcg agcaaaattt cttcgataaa
    27121 ggccagatgg cgatgcgatg cttcgcgcgc ttcttccggc ttaccggcca taatcgcttc
    27181 aaatatgcgg gtgcggtgac tactcaccag cggcagcatc tcgcgacgcg aatagagcaa
    27241 ttcgaagttc tggcggacat tctgggccaa catcggctcc atacacctta gcagatgaag
    27301 cagaaccaca ttgtgggccg cttcggtgac ggcaatctga tactggagta cggcgtttga
    27361 ttccgcgtcc agatcgccag actgctgcgc cagctctatg gcgtggtgga gttcacggat
    27421 gcgttccttg tcttcatcgg tactacgcag cgcggcgtaa taagcggcga taccttccag
    27481 ggcgtgtcgt gtttcgagca agtcatactg tgattcagga tggtcggaga gcagctccac
    27541 cagcggatcg ctgaagcttt gccataggct gctctggaca aaagtgccgc caccctggcg
    27601 acgaagcaac aagcccttcg cttcgagacg ttgaatcgcc tcacgcaagg agggacggga
    27661 gacgtcgaac tgttttgcca gttcgcgttc cggtgggagt ttttcgcccg ggcggagagt
    27721 gccttcgagg atcaaaaact ccagttgctg ctcaatcaca tcggagagtt ttggttggcg
    27781 gattttgctg taggccatga gttcctgtct taagccactt gccgaagtca attggtctta
    27841 ccaatttcat gtctgtgacg ctaaagtaac aaagtattca ccttatgtcc atacaggttt
    27901 tgattgaaat catgaaactg tgcacatttt aacaacttga catatataac gtttcaaagt
    27961 tgtaactatg cacaaatgta gactttacgt aggaaaggga ttttttaacg attattaaac
    28021 tataaaaatc cgaattgaac cgattcactt accaattttt tgatttttaa gtcaataaaa
    28081 acgaatttaa attcactctg catattgaga ggggttgagg ctgagcttta caaacggttt
    28141 ctttttaagc aactcatctt caaccatgca taaagcgggt gcattcgctg ccgcatacca
    28201 ttattcttga tctgacagaa gtctttttgt aacaattcaa acttccttga tgtaaataaa
    28261 tcaatacaac aaacggaatt gcaaacttac acacgcatca ctgcgtagat caaaaaaaca
    28321 atcaccgcac gaggtttcat gatggaaggt caacagcacg gcgagcagct aaagcgcggc
    28381 cttaaaaacc gccatattca gcttatcgcg ctgggtggcg cgatagggac agggctattc
    28441 ctgggtagcg cctccgtaat acagtccgca gggccaggga ttatcctggg ttacgccatt
    28501 gctggtttta tcgcctttct gatcatgcgt cagctgggtg aaatggtggt cgaagaacct
    28561 gtcgcaggct cctttagcca ctttgcttat aaatactggg gcagttttgc cggtttcgcc
    28621 tctggctgga actactgggt actgtacgtt ttagttgcca tggcagagct gactgccgtg
    28681 ggtaaataca ttcagttctg gtatccggaa atcccaacct gggtttctgc cgccgtattc
    28741 tttgtggtga ttaacgccat caacctgacc aacgttaaag tgtttggcga gatggagttc
    28801 tggtttgcca ttatcaaagt tattgcggta gtagcgatga tcatcttcgg cggctggctg
    28861 ctgttcagtg gcaacggcgg cccgcaggcg agcgttagca acctgtggga tcaaggtggt
    28921 ttcctgccgc acggcttcac cggactggtg atgatgatgg cgattatcat gttctcgttc
    28981 ggtggtctgg aactggtggg gatcaccgca gcagaagctg ataacccgga gcaaagtatc
    29041 ccgaaagcaa ctaaccaggt tatctaccgc atcctgattt tctatattgg ttcgttagcc
    29101 gttctgctct cactgatgcc gtggacccgc gttaccgccg ataccagtcc gtttgtgctg
    29161 atcttccacg agttaggcga tacctttgtg gcgaatgcgc tgaacatcgt ggtactgact
    29221 gcggcgctct ccgtgtacaa cagctgcgta tattgcaaca gccgtatgct gtttggtctg
    29281 gcacaacagg ggaacgcgcc aaaagcgctg gcgtctgtcg ataaacgcgg cgtaccggtt
    29341 aacaccattc tggtgtctgc gctggttaca gcattgtgcg tattgattaa ctatcttgct
    29401 ccggaatccg catttggcct gttaatggca ctggtggtat ccgcactggt gatcaactgg
    29461 gcgatgatca gtctggcgca tatgaagttc cgtcgcgcca agcaggaaca aggcgtggta
    29521 actcgcttcc ctgctctgct ttatccgctg ggtaactgga tctgcctgct gtttatggcg
    29581 gcggtactgg tgattatgct gatgacccca ggaatggcga tttcggtata cctgatcccg
    29641 gtatggctga tcgtgttagg tatcggctat ctgtttaaag agaaaaccgc caaagccgta
    29701 aaagcgcatt aatctctcta cgccctcacc cgtacagggt gagggcaata atctctacac
    29761 caacgccccg taaatcgtca gtagcgcaat caccaccacg accacgaacg aggttttctt
    29821 cgccattgaa accgctgcct tcggcgtctc caccttatcg acatgcggtt cacgcgccag
    29881 agagaactgc gccagacgcg ttaatacctg atattgcgaa gtatggaaat cacccagcga
    29941 agcaaaccag gccggtaacg ctttctcacc atgaccgatc aaggcatata ccacacccgc
    30001 aagacgaacc ggcacccaat ccagtacatg aagcactgca tcaatgccgg actgtaaacg
    30061 atgatgcggc gtctggtatc tcgccagcca gtattgccat gcacgcaaga acgcataccc
    30121 catcagcgta acgggtcccc aggttccccc cacaatcagc cagaacagcg gtgcaagata
    30181 aaaacgaaag ttaatccaca gcaatgcatt ttgcagctca cgcaaatact cacgttcgtc
    30241 gcagcctgcc gggacgccgt gaatcatggt gagttcgcca gccatcgtgg cacgggcatg
    30301 gctatcatta cgtgaagcag ctgtcagata agcatgataa tgaagacgaa ctttacctgc
    30361 gccaatacac agcaaaccaa tcagcagcca caccagcagc gtgggaacgt tgaacaatac
    30421 tccctgcaat gcgcgtaaca gtaaaaaagt cacacccatc gcaataatgg tcatgcctaa
    30481 cgtgcgcccg agagaaaaat gtttcacccg ccgaaagaac gcttcaagac gatgatcaag
    30541 ctgccagtgc tcgcccaact taaacaggcg ctcgaaaatt aacaccagta aggttgtaaa
    30601 tagcgtcatg ttgtctcctt gctgaccagc gcacgaaacc gtgcccaatc aaatgcagga
    30661 ccgggatcgg ttttccgatc cggcgcaata tcacaatggc ccgtcatgtt attagcgata
    30721 tcgggatagc gatctatcag tgcccgcgta accgccgcaa gctgttgata ctgcgcatcg
    30781 gtatacgcca gagtatcggt gccttcaagc tcaatcccaa tagaaaaatc attgcagcgt
    30841 tcacgcccct gatactgaga gactcccgca tgccaggcac gtttatcgaa aggaacatac
    30901 tggactattt caccatcacg gcgaatcaaa cagtgagcgg agacgcgcaa atgggcgatc
    30961 tcagcaaaga aaggatgtgc ctgcggatca atagttccag tgaataatgc gtcgatccac
    31021 ggaccgccaa actcgcctgg cggcaggcta atattgtgca ccaccagcag ggtgggtgtt
    31081 tcgtcatcca ggcggcaatc gtaatgtggt gagggaacgc ggcgcgcgcc aaccagccac
    31141 ccctgttcta acaacatgca ggatctcctt atatgtggtg ctaatacccg gttcagagta
    31201 gcatgtttct accttatgat tcgttagcta tctggagttt taacatgccg cctcgccgct
    31261 acaaccctga cacccgacgt gacgagctgc tggaacgcat taatctcgat atccccggcg
    31321 cggtggccca ggcgctgcgg gaagatttag gcggaacagt cgatgccaac aatgacatta
    31381 cggcaaaact tttaccggaa aattctcgct ctcatgccac ggtgatcacc cgcgagaatg
    31441 gcgtattttg tggcaaacgc tgggttgaag aggtgtttat tcaactggca ggcgacgatg
    31501 tcaccataat ctggcatgtg gatgacggcg atgtcatcaa cgccaatcaa cccttgttcg
    31561 aacttgaagg cccatcccgc gtgctgttaa cgggcgaacg cactgcgctt aattttgtgc
    31621 aaaccctttc aggagttgcc agtaaggtac gccactatgt cgaattactg gaaggcacca
    31681 acacgcagtt gttggatacg cgcaaaacct tacctggcct gcgttcagct ctgaaatacg
    31741 cggtactttg cggcggcgga gcgaatcacc gtctggggct ttctgatgcc ttcctgatca
    31801 aagaaaacca tattattgcc tccggctcag tgcgccaggc ggtcgaaaaa gcgtcctggc
    31861 tgcatccgga tgcgccagta gaagtcgaag tagagaatct ggaagaactt gatgaggccc
    31921 tgaaagccgg agccgatatc atcatgctgg ataacttcga aacagaacag atgcgcgaag
    31981 ccgtcaaacg caccaacggc aaggcgctac tggaagtgtc tggcaacgtc actgacaaaa
    32041 cactgcgcga atttgccgaa acgggcgtgg actttatctc cgtcggcgcg ctaactaaac
    32101 acgtacaagc actcgacctt tcaatgcgtt ttcgctaatt gctgacagat ttatccgctc
    32161 catcgcgggc ggataatctc cccttcccca actttcttcg taacgcctcg caaatttctt
    32221 gtctttcgct gcaacgaaat aaaaaacctc ggaatctgct ttcccgtttc gttttttgcc
    32281 cctcgcctgt gctgatggcg tttgccaaag tagcaccaac caaatcaagg agcgaaacag
    32341 atggacaagc aacgcggttt tacacttatc gaactgatgg tggttattgg catcattgcc
    32401 attttaagcg ccattggtat tcccgcttat caaaactacc tgcgcaaagc cgcactcacc
    32461 gacatgctac aaacctttgt gccttaccgt accgccgtag agttgtgcgc gctggaacat
    32521 ggtggattag atacctgcga cggtggcagc aatggcattc cctcgcctac caccacccgc
    32581 tatgtttcag ccatgagtgt ggcaaagggc gtggtgtcgc tgaccgggca agaaagtctc
    32641 aatgggctaa gcgtcgtcat gacgccaggt tgggataacg caaacggcgt caccggctgg
    32701 gcgcgcaact gcaatattca aagtgacagc gcattgcagc aagcctgcga agatgtcttc
    32761 cgctttgatg acgccaacta aggagcggca atgaatattc cacagctcac ggccctgtgc
    32821 ctgcgttatc agggagtctt gctggatgcc agcgaagaag tggttcatgt tgcggtggtc
    32881 gatgccccct cacatgagtt gctggacgca ttgcatttcg ctaccaccaa acgtattgag
    32941 atcacctgct ggacgcgcca acaaatggaa ggtcacgcca gtcgcacaca acagacattg
    33001 cccgtagctg ttcaggagaa gcatcagccc aaagcagagt tgctgactcg aacgttacaa
    33061 tctgcgctgg aacaacgcgc gtctgatatt catatcgaac cagcggacaa tgcctaccgc
    33121 atccgcttgc gtatcgacgg cgtattgcat cctttaccgg atgtttcacc ggatgccgga
    33181 gtcgcattaa cagccagatt aaaagtgctg ggaaacctgg atattgcgga acatcgcctg
    33241 ccgcaggacg ggcaattcac tgtcgaactg gcaggaaaca ccgtctcatt tcgtattgcg
    33301 accttaccat gtcggggtgg tgaaaaggtg gtattaaggt tgttacagca ggtgagccaa
    33361 gcactggatg ttaacacgct gggaatgcag ccgttacaac tggcggactt tgctcatgcc
    33421 ttgcaacaac cacagggact ggtgctggta actggcccta caggcagcgg caaaacggtc
    33481 acgctttata gtgccctgca aacgctgaat accgctgaca ttaatatttg tagcgtcgaa
    33541 gatccggttg agatccccat agccggacta aaccagacgc aaatccatcc gcgtgccggg
    33601 ctcacctttc agggtgtttt gcgtgcgtta ttgcgccagg atcctgacgt catcatgatc
    33661 ggagagatcc gcgatggcga aacggcagaa attgccatta aagccgcgca aaccggtcac
    33721 ctggtgttgt ccacgctaca caccaattcc accactgaaa cgctggtacg tttacagcaa
    33781 atgggggtcg cccgctggat gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg
    33841 gtacgtaaac tttgcccaca ttgtcgccga cagcaagggg agcccatcca tattccagac
    33901 actgtatggc cgtcgccgct gccccactgg caggcacccg gttgtgtaca ttgctaccac
    33961 ggtttttatg gtcgtaccgc cttgtttgaa gtcctgccca ttacaccagc catacgccag
    34021 cttatttccg ctaatactga agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg
    34081 cgtacgcttt ttgaaaacgg ctgcctggcc gtggagcaag gcttaaccac ctttgaagag
    34141 ttaatccgcg tactggggat gccgcatggc aagtaagcaa ctctggcgct ggcatggcat
    34201 aaccggcgac ggcaatgcgc aagatgggat gctatgggca gagagccgta ctttactgct
    34261 tatggcacta cagcaacaga tggttacccc actaagcctg aagcgaatcg ccatcaattc
    34321 tgcgcagtgg cgaggagata aaagcgcgga agtcattcat caactggcga cgctacttaa
    34381 agccgggtta acgctttctg aagggctggc tctgctggcg gaacagcatc ccagtaaaca
    34441 atggcaagcg ttgctgcaat cgctggcgca cgatctcgaa cagggcattg ctttttccaa
    34501 tgccttatta ccctggtcag aggcatttcc gccgctctat caggcgatga tccgcacggg
    34561 tgaactgacc ggtaagctgg atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca
    34621 gcgtcagttg accgacaaag tgaaatcagc gttgcattat cccatcatca ttttagcgat
    34681 ggcaatcatg gtggttgtgg caatgctgca ttttgttctg ccggagtttg ccgctatcta
    34741 taagaccttc aacaccccac taccggcact aacgcagggg atcatgacgc tggcagactt
    34801 tagtggcgaa tggggctggc tgctggtgtt gttcggcttt ctgctggcga tagccaataa
    34861 gttgctgatg cgccgcccga cctggcttat cgcgcggcag aaattgctgt tacgcatccc
    34921 gattatgggt tcactgatga ggggacaaaa actcacgcag atctttacga ttctggcgct
    34981 gacacaaagt gcaggcatta cttttttaca gggcgtagag agcgtcagag aaacaatgcg
    35041 ctgcccgtac tgggtgcaac ttctgacaca aatccagcac gatatcagta acggtcatcc
    35101 catctggctg gcgctaaaaa atgccggtga gtttagcccg ctctgtttgc aattagtgag
    35161 aacaggagag gcatccggct cgctggacct catgttagac aacctcgccc atcatcatcg
    35221 ggataacaca atggcgctgg cggataacct cgcagcctta ctggaaccgg cgttgctgat
    35281 cataacggga ggaattatcg gtacgctggt ggtggcaatg tatctgccaa ttttccattt
    35341 aggcgatgcg atgagtggga tgggataacg ctggcgtgtt gctccacgcc agcgttggga
    35401 gattacaggt tgttgaagat gcggttttct tgttccagca cacgaataaa cgtggtgcgc
    35461 ttggtcagct ctttcaggcg tgaagcccca acgtatgtac aagctgaacg caggccgccc
    35521 aaaatatcac gcgcggtatt ttcaaccggg cctcgcagcg gcagcttgac ggttttacct
    35581 tctgctgcgc ggtattccgc aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc
    35641 atgccgtaga acagcataaa tttctcgccg ttctcctcaa cgatgcgacc gccgctctct
    35701 tcgtggcccg ccagcatgcc gccaagcatg acgaaatcgg caccgccgcc aaaggctttc
    35761 gccacatcgc ccggcgtggt acagccaccg tcgctgacga tcattccgcc cagaccgtgc
    35821 gccgcatcgg cacattcgat taccgcagaa agctgcggat aaccgacgcc tgttttgacg
    35881 cgagttgtac aaacagaacc tgggccaatg ccaactttaa cgatatcagc accggagagg
    35941 ataagctcct cacacatttc accagtcact acgttgccag cacaaatggt tttggtcggc
    36001 cacgcttcac gcgctttcgc aacgaactgc acgaagtgtt cggaataacc attcgccaca
    36061 tcaatacaaa cgaagtttaa tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa
    36121 tccgcatcag acgtgccggt agaaaccatc acatgtttca gcacatcagc ggaagaattg
    36181 ttgataaacg cttgccactc ttcgacagaa aagtgtttat gcacagcagt cagaatatca
    36241 aaagaagcca gcgcagaggc catagaaaat gtgcctacgg tgtccatatt tgcggcgata
    36301 atcggcacgc cggaccagct ctgacctgaa tgtttgaagg tgaattgacg ttccagttca
    36361 acatcggaac ggcttttaag agtggagcgt ttagggcgga tgagaacgtc tttaaaacct
    36421 aacttcagat cttcttcaat acgcatgtgc ggattcctgg ggttaatggc tataagggta
    36481 agagcgcaac tccagtgacg ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa
    36541 ttgtcttttt ttacgctaca atcccataaa tttacctggt aaatagtgag cgaacgtttc
    36601 atcaatttca ctttcgcgcg ctcattttta acgttttgat aatcatgatg aattcccggg
    36661 aatataaatt atggggtata tagttgcctt aacgggaggc attggcagtg gtaagagtac
    36721 cgttgccaat gcgtttgctg atctcggaat taacgtcatt gatgccgata ttattgcgcg
    36781 tcaggtggtt gaaccaggtg cacctgcgct acatgccatt gctgatcact ttggcgaaaa
    36841 catgattgct gctgatggaa cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa
    36901 cccggaagag aaaaactggc ttaacgacct gctgcatccg ctgattcagc aagagacgca
    36961 acaccagatc cagcaagcaa cttcccccta tgtactgtgg gttgtgccat tgctggtaga
    37021 aaactcactg tataaaaaag cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca
    37081 acttaagcgc accatgcagc gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc
    37141 tgctcaggca acgcgcgaag cccgccttgc cgtggcagat gacgtcattg ataataacgg
    37201 cgcaccggat gctatcgcat cggatgttgc ccgcctgcac gcacactatt tgcagcttgc
    37261 gtcgcagttt gtctcacagg aaaaaccgta atgcagaccc aggtcctttt tgaacatcca
    37321 ctaaatgaaa aaatgcgtac atggctgcgc attgagtttt tgattcagca actcaccgtt
    37381 aatttaccca tcgttgacca cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta
    37441 ctggatgttt tcgagcgcgg cgaagtccgc actgagctgt tgaaagaact taaccggcag
    37501 caacgtaaac tccagacctg gattggcgtg cctggcgtgg accagagccg tattgaagca
    37561 ttaattcagc agttaaaagc ggcggggagc gtattaattt ccgcgccgcg tatcgggcaa
    37621 tttctgcgtg aagatcgttt gattgctctg gtgcgtcagc gactgagcat cccaggcggc
    37681 tgttgcagct ttgatttacc tacattgcac atttggctgc atctacccca ggcgcagcgc
    37741 gacagccagg tagaaacctg gattgccagc ctgaacccgc tcacccaggc acttaccatg
    37801 gtgctggatt taattcgcca gtctgccccc ttccgtaaac aaaccagcct gaatggtttt
    37861 tatcaggata acggtggcga tgccgacttg ctgcgcctga atctgtcgct cgattcacag
    37921 ctttatccgc aaatttccgg acataagagc cgttttgcca ttcgttttat gccgctggac
    37981 actgaaaacg gacaggtacc ggaacgtctg gatttcgaac tggcctgttg ctaaggagta
    38041 aaaatgtcag aaactattac ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt
    38101 gaaatcagcc cgtttcggcc attttgctcc aaacgttgtc agctgatcga cctcggagaa
    38161 tgggctgctg aagaaaaacg aatccccagc agtggcgatc tttccgaaag cgatgactgg
    38221 agcgaagagc caaagcagtg acatttgtct gatgcctcac gtaggcctga taagacgcgg
    38281 acagcgtcgc atcaggcatc ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccaac
    38341 ctacagacgc ttaagcttcg caattaccgg ttcattggct ggcggaaaat catcggcatt
    38401 aagaccgacc agcgacatcc actcaccggg ttgcccttct ttaccccacg gcaccccttc
    38461 ccaactttcg accagccaga accacagagt tatatgcctg tccgggaatt catattccag
    38521 tttttcaaat agcgaaaaat gttgtggggt aatcccgacc tcttcctgaa gttcacgcac
    38581 caccgcctgt tccggcgttt cgcccatttc aattttaccg ccgggaaact ccagtttatt
    38641 cgccatgtgc gcatctgctg cgcgacgtgt tataaagatt tcattgttct cgttgcgaat
    38701 aatacctacc gcaatttgca gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat
    38761 cctgcgcctt ttacttcaac tgttagcttt tattgcaggc gaccatggca ctgcttgtat
    38821 tttttaccag aaccgcacgg gcaaggatcg ttacgtccta ctttgcgttc accggtttgc
    38881 gccgccagtg cagctgcggc tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt
    38941 gctaaacgct cggcttccat acgacgctgt tgttccagct cctcaacctc ttcaggcata
    39001 cgtacctgaa ctttgctcag cgtactgata acttcatatt tcaacgactc cagcatcgct
    39061 gcaaacatgg agaacgattc acgtttgtat tcctgcttcg gatctttctg tgcatagcca
    39121 cgcaggtgga taccctgacg cagatagtcc atcgctgcca ggtgctcttt ccacagggag
    39181 tcaagcgttt gcagcatgac gcctttctcg aagtgacgca tcatctcagc accaaccact
    39241 tcttctttac gctgatacac ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct
    39301 tcatgcagtt ctggttcttt atccagccac tcggcaattg gtaaatcgag gtcgaaatcg
    39361 ttcttcagac gttcctgcag ccccggaata tcccacattt cttccagcga ctgtggcgga
    39421 atgtaggcat caatggtggc tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc
    39481 acatcgctga catccagcag ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta
    39541 gccacgtcat catattccag cagttgctta cgaatgtcga agttacgact ttcaacttta
    39601 cgctgggcgt tggcaatcgc tttagtcacc cacgggtgtt caatggcttc gcctggcttc
    39661 atacccagtt tacgcatcat gccggatact cggtcggaag caaaaatacg catcagcgca
    39721 tcttccatcg acaggtagaa acgggaagaa ccagcatccc cctgacgacc agaacgaccg
    39781 cgcaactggt tatcgatacg acgggattcg tgacgctcgg taccgatgat atgcaggcca
    39841 cctgcttcca gtaccgcatc gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc
    39901 tctgcggtcg gattttccag cgcggcaact tctgcctgcc agctacctcc gagcacaata
    39961 tctgtaccac gacccgccat gttggtcgcg atagtcaccg cagccggata acctgcctga
    40021 gcaacaatcg ccgcttcgtt ggcgtggaat ttggcgttca ggacattgtg cttaataccg
    40081 gctttggtca gttcgtttga caccagctcc gatttttcga tggagatagt acccaccagc
    40141 accggctggc ctttcgcagt acgttctttg atatcttcaa tgatcgcctg aattttttcc
    40201 gcttcagtca tgtagaccag gtccggcaga tctttacgaa tcattggacg gttggtcgga
    40261 acaacgacgg tatccagttt gtagatggag ctaaattcga aagcttcggt atcagcagta
    40321 ccggtcatcc ccgccagttt ttcatacaga cggaagtagt tctggaaggt gatcgaagcc
    40381 agcgtttggt tttcgttctg gatctgcaca ccttctttcg cttccacagc ctggtgcaga
    40441 ccatcggacc agcgtcggcc ctgcatggta cgaccggtgt gttcgtcaac gatgataact
    40501 tcaccatctt taacgatgta gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc
    40561 gccgttacgt ggtgcatcag catgatgttg gccggagagt acagagactc cccttcatcc
    40621 atgatgccct ctttcaccag cagttcttca atcagcacca gaccacgttc ggtcaggttc
    40681 acctggcgag atttttcgtc caccgagaag tggccttcgc cctggaaggt ttcggagtct
    40741 tctttttcct gacggatcag gtgcggaata attttattca cgcgtttata catttccgag
    40801 ctgtcttctg ccgggccgga aatgatcagc ggtgtacgcg cttcatcgat caggatggag
    40861 tccacttcgt ccaccagcgc atagtgcagt ttacgctgta cacgttcttc agggctgaac
    40921 gccatgttgt cgcgcaggta gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca
    40981 gctgcgtaag cttcgcgctt tgccggtgct ggcatgcccg gcaggttgat accaacagtc
    41041 aggccaagga attcaaacag cggacggttg ttttcggcgt cacgttgcgc caggtagtcg
    41101 ttgacggtaa ctacgtgcac gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt
    41161 gcggtcaggg ttttaccttc accggtacgc atttcggcga tgcagcgttc gttaagaacc
    41221 ataccgccga gtaactgaac gtcgaagtga cgcataccaa agacgcgctt acttgcctca
    41281 cgcaccacgg cgaaagcttc cgggatcaga ttttccagca cttcgccttt ttccagacgc
    41341 gcacgaaact ctgcggtttt ccctttcagt tcttcatcgg agagtttttc catctccggt
    41401 tccatggcat tgatgatgtt gaccactttg cgcatccggc gcagggtgcg atcgttacga
    41461 ctaccgaaaa ctttagttaa caatttgatt agcataataa aatctcaaac gccccgcgtt
    41521 acggagttaa taaaatgaag taaaggttta ttgttgttag gtgaggcgtt gagggccagc
    41581 acggatgcct tgcgcctggc ttatccagac gggcgtgctg aattttgctt gtggggtaaa
    41641 atgcgcataa tcaatgcgat aacccttttc agacggcgtg ccttcctggg tcagcagcgc
    41701 gctgagcgta tccagtaatg caagatgttg cgcctgaaga ggcaaagatt cttcagcaac
    41761 gggcagtgtt tgcggtgcca ttgcgaaaga aagatgacgg attaccgtgc gaatagcatg
    41821 ttgatgccag taatcaacgg aatagttcga attcgggcgg cgcgtgttcg cttccagcaa
    41881 ggccaattga ccaaagttaa ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc
    41941 gggcgcgttt ggttcggcgg cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat
    42001 ccctaataag agatgcggcc agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat
    42061 tccactcacg ttattgccat cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc
    42121 attaaagcct gaagtccaca gcacgaatga agataagttg tgcgtaaaag tgcttaagaa
    42181 aacagcgttc gcaccgtttt ttatagctct tataaaattc aaaaaaaaac gactggttca
    42241 cctggccgga gagagtgcca gatttgccag tcgaatttta tacgacagta taaatgtcgt
    42301 tatgccagta cagctgaagg cgctttgaag gccaacggca gttctgcgtc gtcctggaag
    42361 gtcacatatt cccaggcttc ctgtttcgcc aggacagcct gcagcagttt gttattcagt
    42421 gcatgaccgg atttataagc ggtaaatgca ccaataatat tgtgaccaca catgaacaag
    42481 tcaccgatcg catcgagcat tttgtgacgc acaaattcgt cttcaaaacg caggccgtct
    42541 tcgttcagta cgcgataatc gtcaacaacg atggcacaat cgaagctgcc gcccaggcac
    42601 aaaccacggg actgcagata ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg
    42661 atctggcgca taaacgcatc agcggagaag ttcatcgcat agcgctggtt gctggaatca
    42721 atagccggat ggttaaaatc gatggtgaaa tccagcgaaa aaccattgta cggcttaaat
    42781 tcagcccact tatcgccatc ttcgacacaa acagtctctt tgatgcgaac aaattttttg
    42841 gcgcagttca actcgtcgat accggcgtca agcagcaggt atacaaacgg agcggcgctg
    42901 ccgtccatga tcgggatttc cggcgcgtta acttcgataa caatgttatc gatgcccagg
    42961 cccgcgagag cagcattgag gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc
    43021 agacacgtac agagcatggt atcacgcaca gatttggcat cggccgggaa atctaccggt
    43081 ggattcaagt cggtgcgacg atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc
    43141 agggtgactt tcttgccggt atgtaaaccg acacccgtcg cctgaacgat acgtttaagt
    43201 gtcctttgtt tgatcatcgt attatctcgc caaattacct atccaaccga agtgtactat
    43261 acattcggcg ggccagttta gcacaaagag cctcgaaacc caaattccag tcaattctta
    43321 atcagcttgc ttacgtagga atgctgggat atccagataa tccggctctt tcgcagtttg
    43381 cggcgcattg tcattcacta ctttagcaac cggcttctgc tcctgggtca gcggagccat
    43441 cccatgctgc tggtagcgat ccatcactgg ctgctgaacc tgcttattgg tcaccagagt
    43501 gatttcagga cgtttgtcca tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc
    43561 attcatatcc gggtcaagag aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc
    43621 acggatggtg ttacctaccg tttcgaactc atccagacgc aggtcgaagc ccgccgtgat
    43681 gttaaccagc acgccgcgcg cgccagacag gtcgatatct tccagcagcg gagaagagat
    43741 agccatttca gcagcttctt ccgcacggtc ttcaccgctc gccacgccag aacccatcat
    43801 tgcgtagccc atttcagaca ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc
    43861 cggacgagta atcagttcgg cgataccttg cacagcgcct ttcagtacat cgttcgctgc
    43921 gccaaacgca tccagcaggg agataccgcg gcccagaact ttcagcagtt tgtcgttcgg
    43981 gatagtgatc agagagtcca catgcttgga cagttcagtg atcccctgct cggcgaatgc
    44041 catacgcttc ttgccttcaa agttgaaagg cttagtgacg acagcaacgg tcaggatacc
    44101 caaatctttt gccacttcag cgacgactgg tgctgcacct gtaccggtac caccacccat
    44161 acccgcagca ataaagacca tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc
    44221 ctcatcagcc gcattgcggc caacttctgg attagcgcca gcgcccagtc ctttggtgat
    44281 accgctaccg atttgaatcg tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt
    44341 atttaccgcg aagaattcaa caccttcaat acgctcgcgc accatgtgtt caacagcatt
    44401 accgccgccg ccgccgacgc cgatgacttt aatcaccgcg tcattggtaa gttccattgg
    44461 ttcaaacata gtttctctcc gatttgtgcc tgtcgcctga ggccgtaatc atcgtcggcc
    44521 tcataaaaat taaaactctt ttcgcagcca actattgagt cgcttgatcc acgagccaac
    44581 tgatgctgta acacgttttt ctacttcagc ttcaccgtta agatgtgact ctttcccata
    44641 gtgaagcaat cccaccgccg tcgaataata cggctcctga gcataatccg ttaaaccggt
    44701 aatgttcagc ggcgcgccga tacgcacttg cgtatgaaac acgcgctgag cacaggctgc
    44761 aagaccttcg atctgcgctg cgccaccggt taatacaatg cctgccgcca ggtgatgttt
    44821 aaccccttgt tggcgaagct tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag
    44881 caactcggta tagcgcggct cgatcacctc tgccagggtc tgacgttgca gactccgcgg
    44941 cggacgaccg cctacgctcg gcacttccac gctctcatct tttccaacga tggaacccag
    45001 cgcacaaccg tggcgaactt taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta
    45061 agcgatatca ctggtcacga cattgccagc ataaggaatt accttagtgt ggcgcaatgc
    45121 cccaccggta taaacggcga tatccattgt accaccaccg atatcgacga cgcagacacc
    45181 cagttcacgt tcatcttccg tcaataccga ataacttgat gccagtccgg caaatatcag
    45241 ttggtcaact ttcagcccac aacgttcaac cgctttgacg atgtttttcg ccatatcgtt
    45301 gtgacatgtg atcaggtgca cttttgcctg catccgcaca cccgaaagtc ctaccggatt
    45361 cttgatccct tcctgatagt caatcgcata ctcttgcggg atcacatgca gcacacgatg
    45421 ctcatcgcgc acacgcaccg atttcgcggt atggacgacg ttttccacat cttcttgcgt
    45481 cacttcttct tcagaaatag gcaccatacc aatttcattc tggcagctga tgtgcttacc
    45541 agaaagcgcc agatataccg aagagatctg acaatctgcc atcaattctg cctggtcaat
    45601 ggcgcgttgt acgcacttga ccacggattc gaggtcgttc accccgcctt tatccatacc
    45661 acgtgacggg cagctgccca cgccaatgat attgaccata ccgtcgggca gaacttcccc
    45721 tactaaagcg gcaaccttcg cggtaccaat ttccagtcct actaccagtt ttctgtccgt
    45781 cgccttgatc attgttgttc tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc
    45841 ggcaagggcg cccagcctac tgccgctcca gagtcataac gcaaatcaac gtagctaatc
    45901 cgtttgccat cggtttgcgc ctgctgctgt aaaaccggat aaagttctac aaagcgagcc
    45961 aaacgtttca tcgtatcgcc ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac
    46021 tgccaggaac gccgcgcggt catcgccgct tccttcagag taaatctgtc ctttgccagc
    46081 atctgcccca tttcgcgata gccctgcaac acttcattag cgctgccttc cgggccatac
    46141 agcattggaa gcacctgctt gctggtgcga tctggcggca cgctgaaggt gtttccttcc
    46201 gcgtctacca tgtgttgatc attccaccgc gcaatcggca catattcaac cagatgaatc
    46261 ttcaattcat caggccactg ctttctgacg ctcacctgct taatccacgg caggcgttgt
    46321 tctatttgcg tctggatgat gttgacatcc tgggtcataa aggtacccgg ctcacccaat
    46381 gccaggatcg actgccggat atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc
    46441 agctttgaga gcggcaggcg ttgcgcatct tccatccagc tcaacacgac ccagccgctc
    46501 accaacactg tcgttaaaac ggtcagcagg aaaaggatcc ccgccagacg cgttccatta
    46561 ttgcggcgag aagaaacctc ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata
    46621 ttagtccgcc agttccagaa ttcgtactac caactgcgag aagctcatac ctgcctgacg
    46681 tgccgccatc ggcaccaggc tgtggctggt catacccggt gaggtattgg cttccagcag
    46741 ataaaactgt ccatcactgt ccagcataac gtcaatacgt ccccatcctt tgcaacctaa
    46801 cgtcgtccat gctttcagca ctaatgcctg caaattggcc tcttgtgacg ctttcagacc
    46861 tgcggggcag aaatactgtg tctcatcaga gagatacttc gcctcataat catagaaggt
    46921 tccggacggt tgaatacgta ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa
    46981 ctccggccca cttagccatt tttcaatcaa tacttcttca tcgtgctgaa atgccaatct
    47041 taatgcatct tgtagagcat tttctgctac tacttttgac attcccacac tggaaccttc
    47101 gcggctcggc ttaacgataa ccggcaaacc cagagcagaa atttctgcta actgcttatc
    47161 gctcaggcct ttttcaaact ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc
    47221 ggcaccttgc cacagaagtt tgctgcgtag tttatctatt gaaagcgcag atgccatcac
    47281 tccgctgccg gtataaggca agcccatcag ctcgagcatg ccctgcagcg taccatcttc
    47341 accgccgcga ccgtgtagcg cgataaacac tttctgaaaa cccatcgact tcagttgcgt
    47401 cacgtcgact tctttcgggt cgacaggata cgcatcaata ccgccttcac gcagcccggc
    47461 taacaccgct gcgccagaat tcagagaaac ttcccgctca gcggaagtcc cgcccaacag
    47521 gaccgcgatt ttatcagtca tgttgttctt cctccggagt ttgcggcttc agtttgattt
    47581 cagctaaaga acgggcaatt tttccgatat taccagcccc ctgaacgaga atcaggtcgt
    47641 taccggttaa taccggtgcc agcatctcgg ctacctgcgc agggtccggc accagaatgg
    47701 gatcaatttt cccacgtcca cgaattgtgc gacacagcga acggctgtcc gctcccggaa
    47761 ttggcgcttc gccagccgga tacacttcca gcatcaacag ggtatcaacc tgcgtcagca
    47821 cattggcgaa atcatcatac aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca
    47881 ttaccaggtt tttatccggc cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg
    47941 tcgggtggtg gccgtagtca tcgaccagca ttgccgtacc gcttttacca ttcactggct
    48001 ccagcgggaa ttcaccgagg aaatcaaaac ggcgaccagt cccctggaag ctctccagcg
    48061 cacgcaaaat agcctcgtcg tcaattccct cttccgtagc aaccgcaacc gcagctgcgg
    48121 cgttcagcgc gttatgacgg cctggcgcat tcagggtgac gcgcatcggc tctttgtcct
    48181 gacgcagcag cgtaaagtgc ccctgcgggc caatctgctg ataatcttct acacgcacgt
    48241 cggcatcttc gctgaagccg taagttgtgg tctgacgtcc cacacgcggt aacaattcgc
    48301 ggatcaccgg atcatcaaca cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa
    48361 aattaataaa ggtctgtttt aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg
    48421 cttcgatatt ggtgacaatc gccaccatcg gttgcagatg caggaacgat gcatcactct
    48481 catctgcttc ggcaatcagg taccgaccat gccccaaacg cgcatgaacc cccgccgctt
    48541 ttaccagccc gccgttaacg aaggttgggt cgagccccgc ttctgcgtag atgctggaaa
    48601 ccatcgcggt agttgtcgtt ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac
    48661 gcattaactc agccagcatt tcggcacgac ggatcaccgg aatacgcgct tcatgagctg
    48721 cgacaatttc cgggttgtcg gcagaaatgg cgctggaaac aacgaccacg ctggcatcac
    48781 gtacgttttc cgggcgatgg ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg
    48841 tgaccggatt tggcgctaaa tcggaaccac tgatctgata accttcattg gccagaactt
    48901 cggcaatacc gcccataccg gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac
    48961 gcatttcggg cacgatggaa cgcagttttg ccaattgttg tgtattcatt ctttacgcca
    49021 ttaacttctt aaattcatac gatgcaaaag gcatcgctac aattacgccc gggcagcccg
    49081 gctcacttca tttgccactc gctcggtggc atccggaatg gatgcagcgc gggcgcgttc
    49141 tgccatggtt aataaggttt ctcgcgacca cccggccagg gtgttggcga cagcatccac
    49201 gctaagctgt ggctgctcga taattttggc tgcgcccgct ttttccagcg gtagcgcatt
    49261 ccagtattgc tggcggtctt tatgttgaaa cggcacaaac aacgccggta gtcctgccgc
    49321 ggcgatttca ctcacagtta aagcaccgga gcggcaaacg acgacatccg cccacgcata
    49381 cgccgccgcc atatcatcaa taaattcagt cactttatgc tgcggttgcc ccgcttcggc
    49441 atacgcctgt tcaacggatt gttgcgaacc tttgccgctc tgatgccaga tagtgactga
    49501 atcacccagt ttcgcagcaa cctgcggcat tgtctggtta agaatgcgtg cgccctggga
    49561 accacccact accaacacac gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa
    49621 cggcagcgcc agcacatcgg tacgcaccgg gttacccacc acttccgcat tagggaaagc
    49681 gcctggaaac gcctgcatca ctttggtagc aatcttcgcc agccatttat tggttaagcc
    49741 cgcaataccg ttttgttcat gaagtacaac cggaatgcct aacgaccacg cggccagacc
    49801 acctggacct gatacatagc cgcccatacc gagtaccacg tcaggtttgt acgctttcat
    49861 aatcgcccgc gcctgacgcc aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt
    49921 tatacctttt ccacgcaaac cagagatacg aatgaaatca atttcgatgc catgttttgg
    49981 cactaagtcc gcttccatac ggtcggcagt ccccagccag cgaacttgcc aaccctgagc
    50041 cattagatag tgcgcaaccg ccagtcccgg gaatacatgt ccaccggttc cgcctgccat
    50101 caccattaat cgctttcctt gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc
    50161 tccagacgcg tttcataatc aatacgcaac agcatcatga tggctgtcga cataatcagt
    50221 aagctcgaac caccgtaact gattagcggc aatgtcagac ctttggtcgg taacatcccc
    50281 gccgccgcgc ctacgttaac cagcgcctgg aagctaaacc agatgccaat agaacaggcg
    50341 agaaaaccgg aaaaacggtg gtcaatttct aatgctttgc ggccaatcga catcgcacga
    50401 aaagcgacga agaataccat taaaagtgct agtaccacac cgacataacc taattcttcg
    50461 ccgataatgg cgaaaataaa gtcagtgtgc gcttccggca gatactccag tttttgtacc
    50521 gaattaccta aaccttgccc ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt
    50581 aactgatagc cgctgccaaa gggatcttcc cacgggttcc agaatgcggt aacacggcgg
    50641 atacggtacg gttcggcgag gatcagcaac acaaccgctg aaatgcccat accgataatg
    50701 gcaatgaact gccacaattt cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac
    50761 aacaccacca ccgtaccaag gtctggctgt gccagcagta acactgccaa caccagaatc
    50821 acgcccatcg gtttcaggaa gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc
    50881 agatagttgg cgatatagca aaacaacgac agttttgtca gctccgcagg ctggatacgc
    50941 agcaaaccga gatcgatcca acgcgatgcc cctttaacag cgctacctac taccagcacg
    51001 atcatcagca ggatgataga tccgagcagc atcgtggcac tgtagcgttg ccagaactcc
    51061 atcggcagac gcagcgtaat gatcgccaga ataaacgcca aaatcagata gacaccatca
    51121 cgcttcgcga agaagaacgg atcgttggtt aagcgttgcc ctatgggcat tgacgccgag
    51181 gtcaccatga taaagccaat cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg
    51241 tacatgatca ggctgtcggt atctttttcc cgcgagccca tcacccagcc ctttagcgcc
    51301 gtggagatcc agaccaagat actgaatcct ggcaggcgcg gcattttcag gcgagggaga
    51361 gataaacgca tcaacctaac tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa
    51421 agttcttgaa ctgatcaagg ctggcacagg ccggggagag cagaaccata tcgcccggct
    51481 gaacacgcgg agccagcaag cgcatagcct gttccatagt ttcggtttgt tctgccactt
    51541 ccgggcgtag cgccgccagc tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat
    51601 cgccattcag gtaacgcgcc agtgggctaa agtccgccga tttaccatcg ccgcccagca
    51661 acaaatgcag cgtgccgtct acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt
    51721 tggtcgcttt tgaatcgtta atccagcgca caccgttatg ctccagcacc acttcaaagc
    51781 gatgcggcag accggtgaag gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg
    51841 cggcatctgc cagcgccagc gccgccagcg cattggtgta gttatgctgc ccggaaagtt
    51901 tcatctcttt cacattcagc actttctcgc ctttaacccg cagccaggtt tcgccctgct
    51961 gatgattcag gtgatagtca cccatgttga cgccaaagct gacgcagcgt tcatccgcac
    52021 cgcgaatcgg cattgttaag gcatcatcag cattaaccac gcaaactttc gcgttttcgt
    52081 aaatgcgcag ttttgctgca cgatactgtt gtaaaccaaa cgggtagcga tccatatgat
    52141 cttcagtcac gttcagaatg gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct
    52201 ggaagctcga cagttccagc acgtacagtt cacactcatc atccagtagc atcaacgcag
    52261 gcaggccaat attgccaccc acaccaacgt taacccccgc cgctttcgcc atttcaccca
    52321 ctagcgtggt gaccgtgctt ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg
    52381 cttcgcgaca aaacagctcg atatcgccaa cgatttcgat tccggcatca gcggcagcgc
    52441 ttaaggatgg atgcgccagt gcaataccgg gactggcgac aatcagatct gccgccatca
    52501 gccattcatc attcagactg cccgtgtggc gttctacggc ttcgggtaat ttatccaggc
    52561 taggcggtgt catacgcgta tccataacgc gcggcgtcac accgcgagcg aggaaaaagt
    52621 ccacgcagga aagcccggtg aggcccaggc cgataatgac aacattttta ccttggtaat
    52681 cagccatgat taacgtacct tcagcgttgc cagaccaatc aggaccagca tcagcgaaat
    52741 aatccagaaa cgcacaatga cgcgcggttc cggccagcct ttcagttcat agtggtgatg
    52801 aatcggtgcc atgcggaaaa tacgttgtcc gcgcagttta aaggagccga cctgcaggat
    52861 gacagaaagc gtttctacca cgaacacgcc ccccataatc accagcagga attcctgacg
    52921 tagcagtacg gcgataatgc ctaacgcacc acctaacgcc agcgaaccga catcgcccat
    52981 aaagacctgc gccggatagg tgttaaacca caggaagccc agtcctgccc cgactatcgc
    53041 ggtacagaca ataaccagtt ccccggcgtg tcgcagatac ggtatgtgta aatagctggc
    53101 aaagttcatg ttgccggtcg cccacgccac cagcgcaaaa ccaccggcga caaatacggt
    53161 cggcataatt gccagaccgt caagaccatc ggtcaggttt accgcgttgc cagtacccac
    53221 aatgacgaag taagccagca gaatgtagaa cagccccagt tgcggcatca catctttaaa
    53281 gaatgggacc accagctgcg ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc
    53341 aacacccagc gcaatgaccg acatccagaa atatttccaa cgagcgatca accctttggt
    53401 atctttacgc accactttgc gataatcatc aacaaagcca ataacaccgt aacctaccag
    53461 caccaccaac acgcaccaga cgtacggatt ggacgggtaa gcccacagca gcacggagat
    53521 cacaatcgct gtcaggatca taatcccgcc catggtcggc gtaccgcgct tgctgaagtg
    53581 tgattcagga ccgtcgttac gcactacctg accaaaggaa agtttttgca aatgagcaat
    53641 catgcgcggg cccatccaca atgagatgaa cagcgcggtc agcaggctga cgatggcgcg
    53701 aaacgtcaga taggaaaaga cgttaaagcc ggaataatat ttgaccaaat gttcggccag
    53761 ccaaactaac atgtcccatt ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta
    53821 cgtgaaccct taactaaaat cgtaattacc tgttgctcag caatcagtga tttaagacgc
    53881 gtaattaacg cagttttatc agcaaaatgt tcgccaacgc cgctggcggt gctgatagca
    53941 tggctttgtt tacccatgct taacacgcgg tcaataccag cagcttttgc cgcctcgccc
    54001 acctgtacat ggcaggcttc gctttcagcg cccagttccg ccatatcgcc caccaccagc
    54061 acgcggtagc ccggcatttc agccagtacc tgaacagctg cggtcattga accgacattg
    54121 gcgttgtagg agtcgtcgag cagcaactgg ttttctgcca gttggatggg gaacagacgg
    54181 ccaggaacag ctttcagatt tgccagcccc gctttgatag catcaagcgt tgcgcccacg
    54241 gacatggaga gcgcagcggc tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc
    54301 agcagaacat cgacgctacc ggttggggtt tgtagggtaa attccatacc gtgcgaggtc
    54361 acatggatat tggtggcggt gaaatcgctg ttggcggcat tgggtgagaa acgccacact
    54421 ttgcgtgagc caattacgct ctgccagttc agccagtcgt tgttgtcggc gttcataatg
    54481 gcgataccgt tttccggcag gccgctaaag atttcacctt tcgctttcgc gacacccgca
    54541 agcgagccaa aaccttccag atgcgccgct gccaggttgt tgaccagcgc cgcttccggg
    54601 cgagtcagac tcacagtcca ggcaatctcg ccctgatggt tcgcgccaag ttcaataact
    54661 gcgtaatcgt attccggcgt taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg
    54721 agattgcctg ccgtataaag cgtgttgccg cactggctta aaatcgccgc cgtcatctct
    54781 ttaacggagg ttttgccgga ggaccccgtc agagcaacca cgcgcgccgg aacttgctgg
    54841 cgaacccatg cagccagttc accaaacgcc agacgcgtat ccttgacgat taactgcggc
    54901 aggtcgatgt ccagcggacg gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg
    54961 tcggcaaaat cgtgggcatc aaaacgttca cctttcaggg caacaaacag gcagcccggc
    55021 gtcagttttc gggtatcagt ggttacagca tcaagggtga tatctgcacc ttgcagttca
    55081 ccgttgagaa tatcggtaag ttggctaagg gttacgctaa tcatgcaatc ccccccagca
    55141 gacgcgccac cgtgacgcga tcggagtagt ccagacgctg attgccaaca atctggtaat
    55201 cctcatggcc tttgcccgcg accagtacca catcattctc tttagcctgc ataacggcgc
    55261 aagtcaccgc ttcagcacgg ccttccatca ctttggcatg tccggcatct aacattcccg
    55321 ccagaatatc gttgatgatg gcacgcggtt cttcggtacg cgggttatcg tccgtcacca
    55381 ccgccacgtc agcaaactct tcggcaattg cgcccatcag tggacgctta cctttatcgc
    55441 gatcgccacc acagccaaag acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct
    55501 gtaaggcttt ttccagtgca tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc
    55561 ctggcgcagt gaacacttcc atacgtccgc aaaccggttg cagacgcgcg gcggttttca
    55621 gcagatcagc cagtggatag cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc
    55681 tgacgttaaa agcgcccatc agatggcttt caatttcgcc atcgccccag cttgaactaa
    55741 agcgaatggt cgcgccgctg tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt
    55801 gacagttcgg attgatatga tcttccattg ataccgcaac cgcgtccggc agttttgcca
    55861 gccagcggcg gcccacttca tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag
    55921 aataaagcag ccatttcgca gcttcgtagt gttccatatc accatgataa tcaaggtgat
    55981 cgcggcttaa gttggtaaag accgacgccg gaaatttcaa tgccgccaca cggtgctgta
    56041 ccagcccgtg ggaggaaact tccattgcgc aaaaagtcgc gccctgatcc accagccccg
    56101 ccagctcatg ctgaacatca actgccgaac cggtagtatt ttctgtcggg atcactttcc
    56161 ccagcagacc attaccaacg gtgcccatta ccgcgctggt ttcgccaagc agttggctcc
    56221 actgcgccag aagctgggtc gtcgtggttt tgccgttggt gcccgttacg cctacgagac
    56281 gtaaattgtc agagggttca tggtaaaagc ggcccgccag tgcagataaa cgctcgttga
    56341 gctggctgag atagatgacc ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct
    56401 catctttcgc ctctgcaata atggcagcca caccttgcgc tatcgcctgc gggatatatc
    56461 gacgcccgtc cgcctgatga cctactacag ctacaaagag atcgcccgcc gcagccacac
    56521 ggctgtcgag tgtcatctct cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa
    56581 gaaggtcgcg caaattacga tctgccacct gtcccctcgc cttgattaat cacaaattca
    56641 tttttatcgc ccgttgtcag cgcatccggc tcgatgttca tggtacgcaa tacgccgccc
    56701 atgatggcac caaagaccgg cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga
    56761 tcgttgataa caacaaccag cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta
    56821 taagcaatat atttattgat gtagcgaccg tccggcccga cctttttcgc ggtaccggtt
    56881 ttaatggcga tacgatagcc tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc
    56941 acgctttcca tcatatgcac cacggtgcga acaatggatt ccgggaagac acgttcaccg
    57001 ggaaccgggg ggtcaacttt ggtaatcgac agtgggcgat aaatgccgta gctgccgata
    57061 gttgcgtaga ctcgcgctaa ctgtaacggt gttaccatta gcccgtagcc gaaagagaag
    57121 gtggccctct ctatgtcaga ccaccgttgt ttttgaggat ataagccact gcgttctccg
    57181 accaacccca aattggtcgc ttttcccagt ccaaaacgtg agtaagtatc tactaacgct
    57241 gaggacggca tcgctaacgc cagcttggaa acaccgacgt tactcgactt ctgtaatacc
    57301 ccggtcaggg ttaattcgct gtagcgtgcc acgtctttga tttcgtggcc gttaattcga
    57361 taaggaatgg tattgagtac cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc
    57421 accaccatcg gtttaaccgt tgagcccggt tcaaacacgt cggtgatggt acggttacgc
    57481 atcgcctctt tcggcgtgcc gctcagattg ttagggttgt atgacgggct gttagccatc
    57541 gccagcactt cacctgtgtt gacatccacc agcacggcgc taccagattc agccttgtta
    57601 aaggccaccg cgttgttcag ttcgcgataa accagcgcct gcaggcgttc atcaatactc
    57661 agcgccaggt tgtgcgctgc ctggctgtca gtagaagaaa tatcttcaat tacgcgacca
    57721 tagcggtctt tacgcacaat gcgctcaccc ggctgcccgg taagccattt atcgaaactc
    57781 ttctcaacgc cctcaatccc ttgactatcg acgttagtaa agccgatgag gtgagcagtc
    57841 acttcgccgg acggatagta acggcgagac tcttcacgca gatgaatccc cggcagtttc
    57901 agttttttga tgtagtccgc catgtcagag tttacctgac gcgccagata aataaagcgc
    57961 cctttcgggt tggcgttaat gcgggcagaa agctgatcca gcggaatatt gagcgcgtta
    58021 gccagcgcct tccagcggtc accgacgctg ataccgccag cgtcatgcac ttctttcggg
    58081 tcagcccaaa tcgcttttac cggcacgctc accgctaacg ggcgaccaga acggtcagta
    58141 atcatgccgc gggaggtgga aacttgctga acgcgaagag aacgcatgtc gccctctttc
    58201 accagcatat ccggggagat aacttgtaac cacgctacgc gtccgagcag aaaagccagc
    58261 gccaggagaa tacagccgca taacaacgca aaacgccaac tgataaagtt ggcatgttct
    58321 tcctgacgtt ttggtttctg cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat
    58381 ttttgcacta cgatattttc ttgtgacgga tcaacatgct gcatttgcag cttttccgtg
    58441 gcgatccttt ccacccggct atggtcgccg agcgcattct cttcaaggat caggttgcgc
    58501 cattcaatgt ctaaagcatc tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa
    58561 cgggtatggt gcgccgtggt taccacggtc accgccgtca aaataatgca aatgaacagg
    58621 cagagtggca gcttcccaaa tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg
    58681 cgctcgtggc ttcccatcga tcctttaact ttgcttagag cttctgtcac tctgctgatc
    58741 atgcgttcgt cctctctgca atacgcagaa ctgaactacg ggcacgaggg ttctcagcca
    58801 cctcttcttc gcccggcatt aacttgccta gtgctcgcag ctgacggcca cccagttttt
    58861 tgagctgctc ttcagtcatc ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac
    58921 gcataaaacg tttcacaata cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc
    58981 cacccggggc cagcacgttg agcgagcttt ttagcgcctg ctctatctcc tccagttcac
    59041 tgtttaccca aatgcgcacc gcctggaagg tacgggtcgc gggatgttta aacttatctt
    59101 tcaccggcgt tgcagcagcc acgacttccg ccagttcttt ggtgcgggtc atcggctgtt
    59161 cgcggttacg ctcgacaatg gcgcgggcaa tgcgtttggc aaaacgctct tcaccatagg
    59221 ttttcaatac ccaggcgata tcggcttctt ctgcggtttg tagccattca gcggctgact
    59281 gcccacgggt tgggtccata cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc
    59341 gttcagcatc atcaagttgc ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct
    59401 tgccaataag atcgcgctcg gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga
    59461 tggagaagcg cggatcatca atagtcttcg caacggcgat agcctgcggg tcgcgatcga
    59521 tcgccagcaa acgcccctct tcgccaagct gcgagaggat cagacgtgag tgaccaccgc
    59581 gaccaaaagt cccatcaatg tagatgccat caggacggat attgaggcca ttaacggctt
    59641 catccagcag caccgtagta tgtttatagt tttccatcat tttatagaga caagtcctgc
    59701 agtcgctccg ataagtctcc ggtagccaac tgctctgcgt cgatatcttc cttgacctgt
    59761 tgatgccagg ttgtttcatc ccacagctca aacttgttga actgtccaac cagcatcact
    59821 tcttttgtca gcccggcatg ttgccgcagt actggcgcga ttaacaatcg acctgcgcca
    59881 tccatctgac attcgctggc atgacctaac agtaggcgct gcacacggcg ctcaaccggg
    59941 ttcatgctcg acagacgcga taatttttgc tcgataattt cccattcagg cagggggtaa
    60001 agcagcaggc acgggtgatg aatgtcaatg gcgcaaacca tttgaccggc agcgttctca
    60061 agcagctgtt cccgataacg ggtaggcact gataagcgcc ctttgctgtc gagattgact
    60121 aacgttgctc cccggaacat gccagcctca ccccttattc ccactttgcc ccacaatttc
    60181 ccactgaaag gagtttacgg agcggaggaa aagcttgtca agccagtcac aaggcatacc
    60241 gaggtaaaaa acccgatgtt tacggctaat atcagcgttg tttattttac gtccaacgtg
    60301 cgaagcaaat taacgttatg aatagctgta agaaaaaatc cctcatcgtc atcactgttt
    60361 taaaatgact cactattttc cacagcaaat ataaagtgtc agtttgcgac gcgagcggca
    60421 ttttaggaca tatcttcccc ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc
    60481 agcgggtttg cgcaaaagca aagaaaagtg tttgttaaat cggcaattca tcgccgcatg
    60541 taacaaaata atgcaaaaac gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg
    60601 tgagttaatt aacgggcggg tttaaaacca tttatcaata atagtcagga atagtcttat
    60661 ttactttaag gatattgat
//
LOCUS       DADMEQ010000011        59523 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.011, whole genome shotgun sequence.
ACCESSION   DADMEQ010000011 DADMEQ010000000
VERSION     DADMEQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.011"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="JNZ05_000699"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="JNZ05_000699"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HAY8564038.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="JNZ05_000700"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="JNZ05_000700"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HAY8564039.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="JNZ05_000701"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="JNZ05_000701"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HAY8564040.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="JNZ05_000702"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="JNZ05_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HAY8564041.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="JNZ05_000703"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="JNZ05_000703"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HAY8564042.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="JNZ05_000704"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="JNZ05_000704"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY8564043.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="JNZ05_000705"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="JNZ05_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HAY8564044.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="JNZ05_000706"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="JNZ05_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY8564045.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="JNZ05_000707"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="JNZ05_000707"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAY8564046.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="JNZ05_000708"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="JNZ05_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HAY8564047.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /locus_tag="JNZ05_000709"
     CDS             complement(14847..15542)
                     /locus_tag="JNZ05_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY8564048.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="JNZ05_000710"
     CDS             complement(15665..16702)
                     /locus_tag="JNZ05_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY8564049.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17034..17522
                     /gene="yhhY"
                     /locus_tag="JNZ05_000711"
     CDS             17034..17522
                     /gene="yhhY"
                     /locus_tag="JNZ05_000711"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY8564050.1"
                     /translation="MSEIVIRHAETRDYETIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17581..19269)
                     /gene="ggt"
                     /locus_tag="JNZ05_000712"
     CDS             complement(17581..19269)
                     /gene="ggt"
                     /locus_tag="JNZ05_000712"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005071965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAY8564051.1"
                     /translation="MLLCSQEVVLAPPVSYGVEEDVFHPVRAKQGMVASVDATATQVG
                     VDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKNGNTTAIDFREMAPAK
                     ATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGTMPLNKVVQPAFKLAR
                     DGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKSGTLVQANLAKSLEMIAE
                     NGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERTPISGDYRWYQVYSMPPPS
                     SGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAYADRSEYLGDPDFVKVPWQ
                     ALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTTHYSVVDKDGNAVAVTYTL
                     NTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANAVGPNKRPLSSMSPTI
                     VVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNAPRFHHQWLPDELRVE
                     KGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGVSDPRSVDDLTAGY"
     gene            19423..19863
                     /locus_tag="JNZ05_000713"
     CDS             19423..19863
                     /locus_tag="JNZ05_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HAY8564052.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19850..20593)
                     /gene="ugpQ"
                     /locus_tag="JNZ05_000714"
     CDS             complement(19850..20593)
                     /gene="ugpQ"
                     /locus_tag="JNZ05_000714"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY8564053.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20615..21502)
                     /gene="ugpC"
                     /locus_tag="JNZ05_000715"
     CDS             complement(20615..21502)
                     /gene="ugpC"
                     /locus_tag="JNZ05_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAY8564054.1"
                     /translation="MADGEFIVMVGPSGCGKSTLLRMVAGLERVTEGDIWINDQRVTE
                     MEPKDRGIAMVFQNYALYPHMSVEENMAWGLKIRGMGKQQIAERVKEAARILELDGLL
                     KRRPRELSGGQRQRVAMGRAIVRDPAVFLFDEPLSNLDAKLRVQMRLELQQLHRRLKT
                     TSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGTPVEVYEKPASLFVASFIGSPAMNLLT
                     GRMNNEGTHFELDGGIVLPLNGGYRQYAGRKMTLGIRPEHIALSSQAEAGGATGASGA
                     PDGRQHAVAASGGKSAASF"
     gene            complement(21582..22427)
                     /gene="ugpE"
                     /locus_tag="JNZ05_000716"
     CDS             complement(21582..22427)
                     /gene="ugpE"
                     /locus_tag="JNZ05_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HAY8564055.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22424..23311)
                     /gene="ugpA"
                     /locus_tag="JNZ05_000717"
     CDS             complement(22424..23311)
                     /gene="ugpA"
                     /locus_tag="JNZ05_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HAY8564056.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23409..24725)
                     /gene="ugpB"
                     /locus_tag="JNZ05_000718"
     CDS             complement(23409..24725)
                     /gene="ugpB"
                     /locus_tag="JNZ05_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HAY8564057.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25122..25835)
                     /gene="livF"
                     /locus_tag="JNZ05_000719"
     CDS             complement(25122..25835)
                     /gene="livF"
                     /locus_tag="JNZ05_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HAY8564058.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25837..26604)
                     /gene="livG"
                     /locus_tag="JNZ05_000720"
     CDS             complement(25837..26604)
                     /gene="livG"
                     /locus_tag="JNZ05_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HAY8564059.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26601..27878)
                     /locus_tag="JNZ05_000721"
     CDS             complement(26601..27878)
                     /locus_tag="JNZ05_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HAY8564060.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27875..28801)
                     /gene="livH"
                     /locus_tag="JNZ05_000722"
     CDS             complement(27875..28801)
                     /gene="livH"
                     /locus_tag="JNZ05_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HAY8564061.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28849..29958)
                     /gene="livk"
                     /locus_tag="JNZ05_000723"
     CDS             complement(28849..29958)
                     /gene="livk"
                     /locus_tag="JNZ05_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HAY8564062.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLACSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30382..30765
                     /gene="panM"
                     /locus_tag="JNZ05_000724"
     CDS             30382..30765
                     /gene="panM"
                     /locus_tag="JNZ05_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HAY8564063.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30964..32067)
                     /locus_tag="JNZ05_000725"
     CDS             complement(30964..32067)
                     /locus_tag="JNZ05_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY8564064.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32338..33192)
                     /gene="rpoH"
                     /locus_tag="JNZ05_000726"
     CDS             complement(32338..33192)
                     /gene="rpoH"
                     /locus_tag="JNZ05_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HAY8564065.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33437..34495)
                     /gene="ftsX"
                     /locus_tag="JNZ05_000727"
     CDS             complement(33437..34495)
                     /gene="ftsX"
                     /locus_tag="JNZ05_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HAY8564066.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIGEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34488..35156)
                     /gene="ftsE"
                     /locus_tag="JNZ05_000728"
     CDS             complement(34488..35156)
                     /gene="ftsE"
                     /locus_tag="JNZ05_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAY8564067.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35159..36655)
                     /gene="ftsY"
                     /locus_tag="JNZ05_000729"
     CDS             complement(35159..36655)
                     /gene="ftsY"
                     /locus_tag="JNZ05_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAY8564068.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36805..37401
                     /gene="rsmD"
                     /locus_tag="JNZ05_000730"
     CDS             36805..37401
                     /gene="rsmD"
                     /locus_tag="JNZ05_000730"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HAY8564069.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37391..37660
                     /locus_tag="JNZ05_000731"
     CDS             37391..37660
                     /locus_tag="JNZ05_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HAY8564070.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37663..38022)
                     /locus_tag="JNZ05_000732"
     CDS             complement(37663..38022)
                     /locus_tag="JNZ05_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HAY8564071.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38163..38789
                     /locus_tag="JNZ05_000733"
     CDS             38163..38789
                     /locus_tag="JNZ05_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HAY8564072.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38863..41061
                     /gene="zntA"
                     /locus_tag="JNZ05_000734"
     CDS             38863..41061
                     /gene="zntA"
                     /locus_tag="JNZ05_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HAY8564073.1"
                     /translation="MSTPDNHGKKAPQFAAFKPITTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41163..41408)
                     /gene="tusA"
                     /locus_tag="JNZ05_000735"
     CDS             complement(41163..41408)
                     /gene="tusA"
                     /locus_tag="JNZ05_000735"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HAY8564074.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41629..42294
                     /locus_tag="JNZ05_000736"
     CDS             41629..42294
                     /locus_tag="JNZ05_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HAY8564075.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42367..42924
                     /gene="dcrB"
                     /locus_tag="JNZ05_000737"
     CDS             42367..42924
                     /gene="dcrB"
                     /locus_tag="JNZ05_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HAY8564076.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42928..44145)
                     /locus_tag="JNZ05_000738"
                     /pseudo
     CDS             complement(42928..44145)
                     /locus_tag="JNZ05_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            44277..45326
                     /locus_tag="JNZ05_000739"
     CDS             44277..45326
                     /locus_tag="JNZ05_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY8564077.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTRVCKIWMETTKGGSKL
                     AILLGPDRPKSRLPG"
     gene            45381..45968
                     /gene="acpT"
                     /locus_tag="JNZ05_000740"
     CDS             45381..45968
                     /gene="acpT"
                     /locus_tag="JNZ05_000740"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HAY8564078.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46079..47653
                     /gene="nikA"
                     /locus_tag="JNZ05_000741"
     CDS             46079..47653
                     /gene="nikA"
                     /locus_tag="JNZ05_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HAY8564079.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPLETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47653..48597
                     /gene="nikB"
                     /locus_tag="JNZ05_000742"
     CDS             47653..48597
                     /gene="nikB"
                     /locus_tag="JNZ05_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HAY8564080.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPTTL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48594..49427
                     /gene="nikC"
                     /locus_tag="JNZ05_000743"
     CDS             48594..49427
                     /gene="nikC"
                     /locus_tag="JNZ05_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HAY8564081.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49427..50191
                     /gene="nikD"
                     /locus_tag="JNZ05_000744"
     CDS             49427..50191
                     /gene="nikD"
                     /locus_tag="JNZ05_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HAY8564082.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50188..50994
                     /gene="nikE"
                     /locus_tag="JNZ05_000745"
     CDS             50188..50994
                     /gene="nikE"
                     /locus_tag="JNZ05_000745"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HAY8564083.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKNLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51000..51401
                     /gene="nikR"
                     /locus_tag="JNZ05_000746"
     CDS             51000..51401
                     /gene="nikR"
                     /locus_tag="JNZ05_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HAY8564084.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51521..51880)
                     /locus_tag="JNZ05_000747"
     CDS             complement(51521..51880)
                     /locus_tag="JNZ05_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HAY8564085.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51877..52152)
                     /locus_tag="JNZ05_000748"
     CDS             complement(51877..52152)
                     /locus_tag="JNZ05_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAY8564086.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(52211..53335)
                     /locus_tag="JNZ05_000749"
     CDS             complement(52211..53335)
                     /locus_tag="JNZ05_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAY8564087.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53335..56070)
                     /gene="rbbA"
                     /locus_tag="JNZ05_000750"
     CDS             complement(53335..56070)
                     /gene="rbbA"
                     /locus_tag="JNZ05_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HAY8564088.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQSFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQLTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56067..57134)
                     /locus_tag="JNZ05_000751"
     CDS             complement(56067..57134)
                     /locus_tag="JNZ05_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY8564089.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKTAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57500..59122)
                     /locus_tag="JNZ05_000752"
     CDS             complement(57500..59122)
                     /locus_tag="JNZ05_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY8564090.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNHGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDSSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGVQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINLGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
BASE COUNT        14066 a        16426 c        15077 g        13954 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggaatgg tttttatctt ccactgcagc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cgaatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccacacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgtttgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tgcctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcaga cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tattacgggt aactgttatc ggtaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctggat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tagcaaaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc
    16861 aatgtgagca atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgagacaat
    16921 aataatcatt ctcattcgca cttgtccaac actttttgca aaaaaatgca tttgactcgc
    16981 atttgaaagt caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg
    17041 agatagtaat acgccacgca gaaacacggg attacgagac catcaggcag attcacgccc
    17101 agccggaggt gtattgcaac acactacagg tgcctcatcc ttccgatcat atgtggcagg
    17161 agcgactcgc cgatcgtccc ggcatcaagc aactcgtcgc ctgtattgat ggagacgtcg
    17221 tgggccatct caccattgac gtgcaacagc gcccacgccg cagtcatgtt gccgattttg
    17281 gtatctgtgt cgactctcgt tggaagaacc gcggcgtcgc cagcgccctg atgcgagaga
    17341 tgattgaaat gtgcgacaac tggttacggg tagatcgcat tgaactaacc gtgtttgtcg
    17401 ataacgcgcc agcaattaag gtctataaaa aattcggctt tgagattgaa gggaccggta
    17461 agaagtacgc attgcgtaat ggtgaatatg tcgatgcgta ttatatggcg cgggtgaaat
    17521 agagaatttg caatctaagt ttgtcattta agccctcacc taccgggagg gcttaagata
    17581 ttaatacccc gccgttaaat catccaccga gcgcgggtcg gatacgccgt acaactcacc
    17641 gtccggccca accataatgc tttgtgtgct gcccatcgcc tctttcagcg ccactttctg
    17701 accttttgct tccagcagct tgagcgtatc cgggctaaac cctttttcga cacgcagctc
    17761 gtccggcaac cactgatggt ggaaacgcgg cgcattggtc gcttcggcga cgttcatgcc
    17821 ataatcgatg ctattcacca ccatttgcag caccgtggtg ataatccggc taccgcctgg
    17881 gctaccggta accagccagg ttttaccgtc tttcaccaca atggtcggcg acatcgacga
    17941 cagcgggcgt ttgttcggcc cgacggcgtt ggcatcaccg cccaccagcc cgtaaacgtt
    18001 cggtacgccc ggtttggcgg agaaatcatc catctggtta ttaagcagaa taccgctctc
    18061 gcccgcgaca atacccgtac cgaaggtggt gttcagcgta taggtcaccg ccaccgcgtt
    18121 accgtcttta tccactacag agtaatgggt agtttgatta ctctcataag gcgcaagctt
    18181 gccagggcga atctcactgg acggcttcgc ttttttgata tcaatttgat cggcaatgga
    18241 tttggcatag gctttattgg tcagtgcctg ccacgggact ttgacaaaat ccgggtcgcc
    18301 aagatattcc gagcggtcgg cgtaggcgta tttctccgct tctgccatga tttgcatcgc
    18361 atcggcgctg ccaaagccgt atttcttcat atcgaagttt tccagaatat tgaggatttg
    18421 tacgatatgg atcccgccgg aggatggcgg tggcatggag taaacctgat accagcgata
    18481 atcgccgctt atcggagtgc gttcgaccgc tttataggcc gctaaatctt ctttagtgat
    18541 caagccaccg tttttctgca tctcctgggc gatctgttcc gcaatcgtgc ctttatagaa
    18601 ttcgtccggg ccgttttcag caatcatctc caggctcttt gccaggttcg cctgcaccag
    18661 cgtgccgctc tttttcagcg gctcgccctc tttccagaag atagctttac tgttttcgtg
    18721 attcggcaac acttcgctac cgtaggtttt gagatcgtca gccagcgcgt cgttaacgat
    18781 aaaaccatcg cgtgccagtt taaacgcggg ctgcacgact ttgttcagcg gcatggtgcc
    18841 gtatttatcc agcgccagcg agaaacctgc taccgtaccc ggtgtgccgg aagccagatg
    18901 cgaagtgagt gattttttgc tgtccgggtt gccctgatca tcgaggaaca tatcgcgggt
    18961 cgctttggcg ggtgccattt cgcggaaatc gatagccgtg gtattgccat ttttcgagcg
    19021 gattaacata aaaccaccac cgcccagatt ccctgcctgc ggatgcgtta ccgccagcgc
    19081 gtagcccacc gccacggcgg catcaacggc attcccgccc tccttgagga tatccacccc
    19141 cacctgagtg gcagtggcgt ccacagacgc taccattccc tgtttcgcgc gtaccggatg
    19201 gaagacatct tcctccacac catacgagac gggcggcgct aaaacaactt cctgagagca
    19261 gagcagcaat ggccacccgg cgtaaaaacg tcggttttat catcgttatt ctccagagat
    19321 taaggggcaa ccccagctaa gcctggtata taactctgaa ttaatcatcg ttttgtcggg
    19381 aagcgagtaa acttaaagga tatcttcaga ggaggatacg cgatgaaacg acttctgatt
    19441 cttacggcac tcctgccgtt tgtcggcttt gcacagccca ttaatactct gaacaaccct
    19501 aaccagccgg ggtatcagat acccagccag cagcggatgc aaacccagat gcagactcag
    19561 caaatccagc aaaaagggat gctgaatcag caactgaaaa cgcaaactca gttgcaacag
    19621 cagcatttag aaaaccagat aaacaataat tctcagcggg tgttgcagtc acagccgggg
    19681 gagcgaaatc ccgcccggca gcaaatgctg cccaacacca acggcgggat gttaaacagc
    19741 aaccgtaatc cggatagttc gttgaatcag cagcatatgc tgccggagag gagaaacggc
    19801 gacatgctga atcagcccag cacgccgcag cctgacattc cgttgaaaac tattgggccg
    19861 taaagttcgg accaatcacg tcaatcgcat cggtacagat gcaatccacg ccccagcgca
    19921 gcaactctgc cgcgcgctgg ggtttattga cggtataaac cagaatccgt aatccggcgt
    19981 ctttcaactg catcactcgc gctttatcga gtaacttatg attgagatga atagagacgc
    20041 agcccagccg cgcggtcagt tcgcgccagt cgtcgcgcca ctcatcaagc aataaaccac
    20101 gcggcagttc cggtgccgcc tgttgtgcag cttctaaagc atcaatctca aacgatgaca
    20161 gcagcggcgg cgtcataccg gcccacagtt ggcgtgccgc cagcgccacc attttgcccg
    20221 ttaatggtcc agtgccagtg gtgggtttga tttcgatatt cgccatcatc ccgtgttcgc
    20281 gacagcgttc cgccacctgc gaaagcaacg gcagcggctc acctttaaac gctttgctgt
    20341 accaactgcc cgcatccacg cgcagtaaat cctgccagtt cagttcaccc gcgacgcccc
    20401 agccgttgct ggtgcgttcg agattgtcgt catggagcag gaagatctcg ccatctttcg
    20461 ataacttcgc gtcaaattcg atcatcttat gaccgtattt tgccccgacg tcgattgccg
    20521 ccagggtgtt ttccggggcc agcttaccgc cgccacgatg agcgacgatg cggggataag
    20581 gccagttact catactcgtt gtcctgtttc accatcaaaa agatgcagct gattttccgc
    20641 cagatgcagc cacagcgtgc tgcctgccgt cgggcgctcc tgatgcgcca gtcgcaccac
    20701 cagcttctgc ttgcgagctt agcgcaatat gttccgggcg aatgccgagg gtcattttac
    20761 gcccggcata ctgacggtag ccaccgttta gcggtagcac gataccgccg tccagttcga
    20821 aatgcgtgcc ttcattattc atgcggcctg tcagcaggtt catcgccggg ctgccgataa
    20881 aactcgccac aaacaggctg gcgggctttt cgtagacttc caccggcgtg ccaatctgtt
    20941 cggcaacgcc gccgttcatc accattactc gctgggcgag cgtcatcgcc tcaacctgat
    21001 cgtgagtaac gtagagtgaa gtcgttttca ggcgacggtg cagctgttgc agttcaagac
    21061 gcatctgtac gcgcagcttg gcatcgaggt tagagagtgg ctcatcaaac aagaacaccg
    21121 ccggatcacg cacaatcgcg cgtcccatcg ccacacgctg gcgctgaccg ccggaaagct
    21181 cgcgcgggcg acgtttgagc agaccgtcca gctccagaat gcgcgccgct tctttaacgc
    21241 gctcggctat ttgctgcttg cccatgccgc gaattttcag cccccacgcc atgttttctt
    21301 cgacgctcat atgcggataa agcgcgtaat tctggaacac catcgcaatc ccgcgatctt
    21361 tcggctccat ttcggtcacg cgctggtcgt tgatccagat atcgccttcg gtcaccctct
    21421 ccagcccggc aaccatacgc agcagcgtcg atttcccgca gccagacggc ccgaccatca
    21481 cgataaattc gccatccgcc acatcaaggg tcagcggttt aatgacctag gttttgccat
    21541 cccagctttt ggttactgcc tgtaatttca gtcctgccat cttatttctc actatcgacc
    21601 aggccgcgca cgaaggcacg ctgcatcact aaaacaatca ctactggtgg gatcagggtt
    21661 aacaacatcg ccgccatcac tgagttccat tccgtggtgc cttcgccagt ggcgatcatc
    21721 cctttgatcc ctgccacggt ggtgccgaga tccacgtcgg taataatcaa caacggccac
    21781 aaatactggt tccagccgta aataaaggtg atcacaaaca gcgccgccag attagttttg
    21841 gagagcggaa aaacgatgtc gcaaaagaag cgcattggtg acgcaccgtc gatccgcgcc
    21901 gcttccacca gttcatccgg cagcgtcata aagaactggc gaaacagaaa cgtggcggtc
    21961 gccgaggcca tcagcggcag cgttaaaccg gcgtagctgt cgagcatctt caggttggcg
    22021 atgacttcca ccgtcgggaa gatacgtact tcaaccggca gcatcagggt gataaaaatc
    22081 atccagaaga agaggttacg tagcggaaaa cgaaaccaga caatggcaaa tgccgagagc
    22141 atcgagacag taattttgcc gagcgtaatg ctgaacgcca tcacaaagct gttaagcaac
    22201 atccgccaga acggcgcgct atttgtgcct accccgttca cccagatgtt gtggatgttt
    22261 tccagcagat gcgtgccggg gatgagcgtc atcggcgcgg cgtagacggc ctgtttatcc
    22321 agcgtcgccg cgacaaacgc cacgtacagc gggaagagga tcaccgcgat cccgaggatc
    22381 agcatggtat ggctgaatat cgtcagccac ggacggttct caatcattgg taacgcacct
    22441 tgctttcaac atagcggaac tgcaccaccg tcagcacgat gacgaggaac atcaacacca
    22501 ccgactgtgc ggcagacgaa gccagatcca gtccggtaaa accttcgcgg tagatcttat
    22561 aaatcagcgt cgtggtggcc tgaaccggcc cgccggacgt ggcggcgtcg atcaccggga
    22621 aggtgtcgaa gaaggcatac accaggttca ctaccagcag gaagaaactc accggggcga
    22681 taagcggcag cgcaatctta aagaagcggc gaatcggccc tgcaccgtcg atggctgcgg
    22741 cttcgatcaa cgaacgggga atggattgca gcgcggcata gaagaacaga aagttgtagc
    22801 tgatttgctt ccacaccgag gcaaacacca ccagaaacat cgcctgaccg ctattttgcg
    22861 cgtggttcca gtcgtagccg aactcagcga gaaaatgggt gatcagcccg cgaccggggt
    22921 taaacaggaa gatccacaat acggcggcaa cggcgggagc cacggcatac ggcagcaaca
    22981 ttaaggtttg gtagaaacga ctgccgcgca cgatgtactc caccagcgcc gcaaagaaca
    23041 gcgataccag caaaccgctg acggtgacaa aggtgctgaa ttttatcgtc gtccagaagg
    23101 agtcgagata atagctgtca tgaaacagcg tgacgaagtt atccagaccg acaaactggc
    23161 tggagaaacc aaacggatcg acgctttgta gcgagtacca caacgcttcg cccgcaggcc
    23221 agataaaaaa gataacggtg atgatgagct gcggcgcgac cagcagataa ggcagccagc
    23281 gcgagcggaa caccggacgg gatgatgaca ttacggttaa ttcctgaact gtgccggatg
    23341 cgcttcgctt atccggccta cacgcgatgc caggtcagat aagcgaaacg catccggcat
    23401 tacactgatt aagacttcgt cgatttctca aagcggcgca gtaactggtt cccacgctca
    23461 actgcggtat ccagcgcctg ctgtggcgtc ttcttaccgg tccacacgct ctcaagctct
    23521 tcatccacaa tcacgcggat ctgcggcatg ttgcccagac gaagcccttt ggtgaacggc
    23581 aacggcggct tgttcagcat ctgacgcgtc gcggtatccg cacctgggtt tttctcgtaa
    23641 aagccctgct cacgggtcag gtcatacgcc gctttggtaa ttggcagata accggttttc
    23701 tgatgccact cagcagcgtt ttctggcttc gcgaggaagt cgagaaactt cgccacgccg
    23761 gtatacgttt ctttatcttt gccctgcatc acccacaggc tggctccgcc gataatggcg
    23821 ttttgtggcg catctttcgc atcggcgtcg taaggcatca tacctacgcc gtagttaaat
    23881 ttggcgtact cgcgaatgtt ggcaagagaa ccggaagagg cggtggtcat cgcgcaatca
    23941 ccgttataga acttctcggt ggattcatcc ttacgaccga cgtagctgaa gtcgcccttc
    24001 ttgttcatct cctcgagcat ggcgatgtgt ttcacctgct ccggcttgtt gaactccagc
    24061 accgcgtccg tgccgtcaaa gccgttgttt ttgctggcaa acggcagacc gttccaggcg
    24121 ctaaagtttt ccagttggat ccagccctgc cagccgctgg cgtagccgca cttaataccg
    24181 gaggctttca gttttgcgga atagtccgcc agatcctgcc aggttttcgg cggctgttcc
    24241 gggtctaacc ccgctttctt gaaggcgtct ttgttgtaat agagaacggg agtcgagctg
    24301 ttgaacggct gggagagtaa gtggccggtt ttgctgtcgg agtagtaacc ggaaaccgtc
    24361 ggcacaaact gcgactcatc gaactgaatc cctgcctctt taaacacgtc atacaccggt
    24421 ttaatggctt tcgacgccat catggtggcg gtgccaactt cataaacctg caaaatagcc
    24481 ggagcgttgc cggtacgaaa tgcggcaatc cccgcgctta aattctgttc gtagttgcct
    24541 ttataggtcg gtacaatttt gtaatccgga ttttcggcgt taaaacgttg ggccagagaa
    24601 tccacctctt tacccagttc cccttccata gaatgccaga acggaatggt cgtcactgcc
    24661 tgtgcattcc ccattaacgc cagtccgagc gccagtgctg aagctgtata atgtaacggt
    24721 ttcatcgttt atctctcttg ttgtaccgaa tgcgcgaatt cacgcgtttt atgctcgcgg
    24781 ggtaacatga catgctcgaa ttacagaaaa ataacttttt tgttacattt gtaagatagt
    24841 aaggtgtcag aaagatgaca aggcggtgac ggcgtgggtg agggaaaatg ggagatgggg
    24901 cacggataag cggaaaaata gaaggtctga atcaaactct acagattact catcgtttca
    24961 tgccggatgc ggcgtaaacg ccttatccgg cctacaagat cgtgcaaatt caatatattg
    25021 caactcaccc agtaggcctg ataagcgcag cgcatcaggc aattttgcat ttgtcacctg
    25081 tctcaaagga gtcttttgac tccttatcaa tcaacgtgtt attacccgcc taaatacgca
    25141 cttctcactg cttcgttcgc cagcagcgcg tcgccggtat cggagagcac cacatggccg
    25201 ttttccagca cgtagccgcg atccgccagc tttagcgcct ggttggcgtt ctgctcgacg
    25261 aggaagatag tcatcccctg ctcgcgcagc tgctcgatgg tgtcgaaaat ttgctggatg
    25321 ataatcggcg caagaccgag cgatggctca tcaagtaaca acaaacgcgg attgctcatc
    25381 agcgcgcgac caatcgccag catctgctgt tcaccgccgg acatggtgcc cgcccgctga
    25441 acacggcgct catgcagacg tggaaacagt tcatacaccc actttatgcg ctcctgaaac
    25501 tggtcacgtt cggcaaagaa gccgcccatc gccaggttct cttccaccgt catccgcgag
    25561 aagacgcgac gcccttccgg gacaatcgct accgcttcgc gcatgatttt cgccgtctgc
    25621 cagtcggtaa tgtctttatc atcaaacaca attcgcccgc tggtggcgcg cggatcgccg
    25681 cataacgtgc cgagcaaggt ggttttcccc gccccgttcg cgccaatgag agtgacaatc
    25741 tcgccctgat tgatatgcag gctcacctca tgcagcgcct ggattttgcc gtagtgggcg
    25801 ctgactttgt caaaggacaa catgactttt tccatcttat gcctcaccta aataggcacg
    25861 gatcacgtcc gggttattac ggatttgctc cggcgtaccg tttgccagtg gcgtcccctg
    25921 attgaccaca taaattcggt ctgaaatccc catcaccagc ttcatatcgt gttcaatcaa
    25981 caagatagtg gtgttgtgat gattgcgcag ttcggcaatc agctcatcca gctctttggt
    26041 ttcttttggg ttaagacctg ccgccggttc gtcgagcatt aaaatctccg gctgcgtcac
    26101 catgcagcgg gcaatctcca gacggcgttg gtcaccatag gccaggttac tcgcctgacg
    26161 gttggcgtgt tccagcaaac caatgcgctc aagccaggtg gcggcgcggt cgagcgcttc
    26221 gctctgggcg cgacggaaag aaggcgtttt caacaggcca gagaacagcc cggttttcag
    26281 ttgctgatgc tgcgccacca gcaggttttc aattaccgtc atttcacgga acagacgcac
    26341 atgctggaag gtgcgcacca cgcccatgcg agcaatttgc tgccccggta aaccttcaag
    26401 atgctgatcg cgcagtaaaa tggtgccgcc ggtgggtttg tagaaaccgg tcaggcagtt
    26461 aaaaaccgtg gtttttccgg caccgttagg gccgattaac gagacgattt cctgcgggta
    26521 cagttcaaga ttgacgttgt tcaccgccag caggccgccg aagcgcatca tcagaccgtt
    26581 aacagataat aatggctgac tcatgcctgc tctcctttcg ctgcgccgtt tttcagcttc
    26641 agttgcgggc gcgtcatggg cagcaaaccc tgcggacgcc agatcatcat cagcaccatc
    26701 aaaccaccga gcattaacat gctgtattcg ttgaaatcac gcatcaattc gcgcgacacc
    26761 accagcagaa cagccgccag aatcactgca aattgcgagc ccataccgcc gagcaccact
    26821 atcgccagca caaaggccga ttcagcaaag gtgaaggatt ccgggctgac aaagccctga
    26881 cgcgccgcaa acagcgttcc ggcaaaaccg gcaaatgcgg cgctgatggt aaaagcggtc
    26941 agcttgatac ggcgcgggct taagcccagc gaacgacagg cgatctcgtc ttcacgcaac
    27001 gcttcccacg cgcggcccag cggcatccgc agcaggcggt taatgacaaa caggcttaac
    27061 accaccagca gcaacgccac caggtagagg aagataacgc gatcggacgg atcgtatttc
    27121 aggccaaaga aattactgaa cgtgtcccag ccgccttcac gagcggtacg gctgaactcg
    27181 agtccgaaga gtgtcggttt cgggatctga ctgattccgt tcgggccgcc ggtgatttca
    27241 gtgttattga gcagcaatat gcgcacaatt tcgccgaaac caagggtaac gatcgccaga
    27301 tagtcaccgc gcagacgcag caccggaaaa ccgagcagga aacccgccgc cgctgccatc
    27361 agtccggcaa tcggcaaaca ggtccagaac cccagaccgt aatagtgatt gagcagcgca
    27421 aaagtgtaag cgccgatggc gtaaaaaccg ccgtacccca gcaccagcag accagaaaga
    27481 ccaacaacca cattcagccc gagaccgagg ataatgtaga tcatggtcag agtggcgata
    27541 tccaccgtcc cacgtgacac cataaacggc cacgccacca caagcaccag cagcgccacg
    27601 aggaacagct tttgcttcac cgtggaccca tcaatcgcgg gcagaataaa cttcggcccg
    27661 gaaacgcttt tcaacccttt ctggaaagcc ggtcgcaaaa gctggaagaa aaagaccacc
    27721 gccgtgccga taaacatcca ctgccaacgg atgtctgaag ccgtgtcgac caccagtttg
    27781 gtgccatcca gctccagttg tacgcccata aagacgcccg ccaggacaaa gaacatcgcg
    27841 gcagagagca gtgccattgc aatatgcatc ggtttcatac tttctctacc tccgggcgac
    27901 ccagaatacc ggtcggcatc accagcagca ccagaatcag cagggcgaat gagaccacat
    27961 ctttatattc cgtactcaga taggcagaag agagcgcctc cgcaatcccc aggatcagcc
    28021 cgccaatcat cgcccctgga atgctgccaa tcccaccgag caccgcagcg gtaaaggctt
    28081 tcatcccggc cataaagccg atataggggt taatgacgca gtagaactga ccgagcagta
    28141 cgccagccac tgccgccatc gccgcgccaa taacaaaggt caaagcaatc acccggtcgg
    28201 tgttgatgcc aagcaggctc gccattttca gatcttccgc gcaggcacga caggcgcgcc
    28261 ccatacggga atagcgaatg aaaatcgtca gcgccagcat ggcgaggaag gtgacaatcc
    28321 agatcaccgc ctgcatggtg gtaatagagg cagagaagtt ttcgctatgg cccaccaccc
    28381 actgaccgtt aaacaggctc ggcagcgcca cgtcgcgcga accttcggtc aggctgacgt
    28441 agttttgcag gaagatggac ataccgattg cggaaataag cgcaatcagg cgcttagagt
    28501 tacgcaccgg gcggtaagct acccgttcga tactccagcc gtaggcgctg gcgatgacga
    28561 ttgcgccgac gaatcccgcc gccaccagca gccagccggt atcaatgccc atcatcatca
    28621 gcgcggcgat gatcataaat gagacgtagc tgccaatcat ataaacctcg ccgtgggcga
    28681 agttgatcat gccgataatg ccgtaaacca tggtgtagcc gatggctatc agcgcgtagg
    28741 tactgcccag cgtgacgccg ttaaacatct gctgcaagaa atacaaaaac tgctcagaca
    28801 taaggtaacc tttctaaacc cgcccgcatt ttacgggcgg tgggatgatc acttggctgc
    28861 cgtggatgaa ccgtcggcgt gccactggaa gacaccaaaa tcaaatccct taagatcgcc
    28921 tttttcatcc cagttcagcg gcccaatcac ggtgtttgca ccgttagctt ttaaatcttt
    28981 caccagcgcc agcggctcat cgctgccggt acgctcaagg gcagttgcca gagattgcac
    29041 tgccgcgtaa gtgatccaga cataaggccc ggacggatct ttcttgtctg ctttcagcgc
    29101 atcaacgatg ccctggtttg ccggatcctg gtcatagcgt tttggcatag tgaccaacat
    29161 gccttcggcg gcatcaccgg caatgttcga cagcgatgcg ttaccgacac cttccggccc
    29221 cataaactgg gttttcaggc caacggaacg ggcttggcgc agcatctgcc ccatttccgg
    29281 gtagtaaccg ccgtagtaaa cgaagtcgat attttctttt ttcaggcggg cgatcagcgc
    29341 ggagaaatct ttctccccgg cggtaatacc gtcgaagaag acgacgttgg cgttagccgc
    29401 tttcagcccg tcctgcaccg aacacgccag cccttcgcca tactgttgtt tgtcgtgaat
    29461 gatggcgatg cgctggggct tcaccgtctc aagaatgtat tttgccgccg taggcccctg
    29521 ggaagagtcc agcccggcag tacgcataat gtgttgataa ccgcgttggg tcagctccgg
    29581 gttggtcgct cccggcgaga tcatcagaat accttcgtct tcgtagatat ccgacgctgg
    29641 ctgggtagag gaagaacaca gatgaccaat aacgtattta atgccgtcgt taacgatttt
    29701 gttggcgacc gcaacggctt gtttcgggtc gcaggcgtcg tcatattcca cgccaaccag
    29761 cttgtcgccc ttaattcccc ctttgacatt aatgtcttta atcgcctgac gcgcgccgtt
    29821 aaattccata tcgccccact gggcaatcgg gccggacatc gcgccgacaa cggcgacttt
    29881 aatatcgtca gccatagcgg tgtgtgaaat tgccagtgca atcatccctg cgatgatagt
    29941 tttcgcattc cgtttcatag tcaaaaatcc ccattcgtga tgttgtgttg ctttgttttt
    30001 atgtgttaac aaatcagact gttctttttt tatactgcac tgtttttgcc tgtctgatta
    30061 aggggttagc gcagtatttt gtgatgatag cgattaaaat ccctattttt cagtcgatta
    30121 agaacagata atattctgaa tttattgata gataaacaga aaaaagtgcc tttgtcagca
    30181 taaaataacg gcacaaaggg cggaataatt cactatcatt caggggatta tgctggacat
    30241 ttttcattct ctaatgtttt aactttgtaa ttattgctgt taaaaaatta atcacctgcc
    30301 aaaagaaata aaaaagagaa agcctccgat taaattattt cgctacactg gttccacttt
    30361 tgtgatttac acgggttacc catgaagctg accatcattc gattagaaaa ctttagcgac
    30421 caggaccgga ttgacctgca aaagatctgg ccggagtatt ccccttcctc tttacaggtt
    30481 gacgataacc accgtatcta cgccgcgcgt tttaacgagc gtctgcttgc ggccgtgcgg
    30541 gtaaccttaa gcggcaccga gggagcactg gattctctac gcgtgcggga agtcacccgc
    30601 cgtcgcggtg tggggcaata tctgctggaa gaggttttgc gtaacaatcc tggcgtttca
    30661 tgctggtgga tggcggatgc aggcgtggaa gatcgcggtg tgatgacggc gtttatgcag
    30721 gcactgggat ttacggcaca gcagggcggc tgggagaagc gttaatcgtc aagttgattt
    30781 caaaagtgat gaccaacgag aaattgcctg atgcgctacg cttatcaggc ctataatgtg
    30841 tgctgcaatt tactgatttc tttggttctt gtaggccgga taaggcgttt acgccgcatc
    30901 cggcatgaag caacgtattc gatattagca atttggcggc aacccaaagt tgccgattaa
    30961 caattacttc gcatcggtcg ccgtgccgtt ggcgtgccag tcaaatacgc cgaactcaaa
    31021 gccctttaga tcgcctttct catcccaggt cagtggcccc atcacggtat ccacggagtt
    31081 cgcttttagg tatttggcga tttcagccgg atcgtcagac tgattcaggc ccgcctgcaa
    31141 agattgcagc gcggcgtagg tggtccagac gaatgcgccg cttgggtcct gttttttcgc
    31201 tttgatcgca tcaacaatgg gtttgttcgc cggaacctga tcgtagttct tcggcttggt
    31261 caccagcagc ccttccgctg attcgcccgc aatgttagac agcgaaacgt tagccacacc
    31321 ttccggcccc ataaactgag ttttcagccc tgccgcgcgt gcctgacgca ggatttgccc
    31381 catttccggg tgataaccgc cgtagtaaac gaagtcgata ttctcttttt tcagacgcgc
    31441 caccagcgtt gagaaatctt tttccccggc ggtgatacca tcaaagaaca ccacgttagc
    31501 attgcctttc ttcaggccgt cctgcaccgc tcgtgccaga ccttcgccgt attgctgttt
    31561 gtcgtgaacg atagcaatac gctgcggttt cactttctca agaatatatt tcgccgccgt
    31621 cggcccctgg tcggagtcca ggccggtggt gcgcaggatc agctgatagc cacgggcggt
    31681 cagctccggc gcggttgccg ctggggtgat cattaaaatg ccttcgtctt cgtagatgtc
    31741 agacgcaggc tgcgttgatg aagaacagag gtgaccaatc acatatttaa tgccgtcgtt
    31801 aacgactttg ttcgccaccg caaccgcctg tttcgggtca caggcatcgt catattttac
    31861 gatttgcagt ttgttgcctt taatgccgcc tttagcgttg atatccgcaa ccgcctgctc
    31921 tgcgccggta aactcctggt caccgtactg cgcaaccgga ccggacattg cacccacgac
    31981 cgcgacttta atatcttctg ccagagccat attgctgaat gccagcgcga tacatcctgc
    32041 cagtaacgct ttacccttta tgttcatcct gagaatcccc attcttctgg ttattacgtg
    32101 tgttgtgatg ttgttgttca gcactttatt tcgttttatg catgactacc cgtgctttag
    32161 cagcatactc tgctaaaaca tacccgattt ttatgatatt ggaatagcta ttttgacagt
    32221 ttattaacaa tctgcgtggg gattggcgtt ttgccggagg gggaattgat tattgtagag
    32281 gcccaaaaaa caaaaacccc ggactctcat ccagggttct ctgcttaata gcggaaatta
    32341 cgcttcaata gcggcgcgaa gttttttcat cgcgttcttt tccagttggc gcacacgttc
    32401 agcggaaaca ccgtaacggt cagccagttc ctgcaacgtg gacttgttgt cttcgtccag
    32461 ccagcgcgca cggatgatgt cctggctgcg ttcgtccaga ccatgcatcg cgtcggtcag
    32521 acggtttgcc gcctgctctt cccagttatc atcttcgatg ccgtcggcaa agttagatga
    32581 tttatcctgc agatagagca ccggtgccat cggctggctg tcggaatcgt cgtcggaaga
    32641 caggtcaaag gtcatgtcct gtgccgccat acgtgattcc atctcacgta cgtctttgct
    32701 ggttacgccc agttcacggg ccaccatttc gacttcatcc tggttaaacc agcccagacg
    32761 ctgcttggtt ttacgcaggt tgaagaacag tttgcgctgc gctttggtgg tcgcaacttt
    32821 gacgatacgc cagttacgca gaacgtattc gtggatctct gctttgatcc agtggacggc
    32881 gaaggagacc aggcgcacac ccacttccgg gttaaaacgg cgcactgctt tcatcaggcc
    32941 gatgttacct tcctgaatca aatccgcctg tggcaggcca tagcccgcat aattacgagc
    33001 aatatgaaca acaaaccgca ggtgagacag gatcagcgtt ttagctgctt ccagatcgcc
    33061 atggtaatgc agcttttcag ccagcgcccg ctcctcgtca gccgacaaca tcggccacgc
    33121 gttagctgcc cggatgtagg aatccaggtt gccaactggg gctaaagcta aactttgcat
    33181 tttgtcagtc attcaaatcc tctcaatcga tatcttctgg cgcttcagtg gtagcaacaa
    33241 ctgtgccaaa gcttaagagc aacgaggtta tcattcactg ttttatcaga ccgtgatttt
    33301 atccacaagt tcaatgcaag cttgtgaata aattacgcac aaaatgtgac atagagatga
    33361 aataccggga agagacaacg gggtctcttt ccctgctacg gaacccattg cagggaaaga
    33421 gtataacacg cttttattat tcaggcgtaa agtggcgtaa atgttgtacc gtggcaagcc
    33481 acgctgccac ccagccaatc atcgagcata ccagcagcaa tagcaggcat tcatcgaacg
    33541 ataaaccatt gatatcaaac ttcgttccga aaacctgtgc cacttccgcc accgctgacg
    33601 acaaacgcag taccagaatt tctgacaaaa ttaatgacaa caatgcgcca gaaaatccca
    33661 gcagcgcacc accgtacagg aacgggcgaa ggatgaaacc atccgtcgca ccaatcagtt
    33721 tctgcacgtt gatggagtca cggcgagcaa agatgctcag acgtacgctg ttgccgatga
    33781 cgaggaacac tgccgccacc atcaacacgc cgatcatcgc cgaaacgcgc ccaaccagcc
    33841 cggtcaacgc cgccagacgg gcaaaccagc tgtcatccat ccgcacttcg ccaatgccgt
    33901 taatctgcgt gatacgatca cgcagcgtat tgagtgattc cgtcccctga aaatcgagtt
    33961 tcgggatcac caccgccact gccggaagcg ggttttcttc cagcatatcc agcgcaccac
    34021 caaaaccaga ccagttacgg aactcgccca gtgcgtcttc acgagaaaga tagttcactt
    34081 tctccacgcc ttgctcggcc tgcaactgtg ccaccacgcc cgcagcggcg tcatcgtcca
    34141 gcgttttttg caagtaaaca gtgatttgcg gtgacggata atactgcgtc gccgcctgat
    34201 taacgttttt gtacaccata taacagacgc tgggcagcgt cagggagatg gcgataacca
    34261 tcaccgttaa aaacgtggcg aacggtttgc ttttcagatc ctgcaatgcg ccgtggaagg
    34321 catagcgcac ctgttcgttg aaaacgttgg ttttgcgatt taccggtttt ggcgaggatt
    34381 tcgcgcgttt cggtgcgtta cgaccgccgt cgcctgagcc gccgaccgat ttacggaagc
    34441 gatccagacg cccaccaaac tgccgaatat gattgattgc atcgcgctta ttcatggccc
    34501 acgcctccat gcaagtgacc atcgctcagg gtgagcatgc gataggaacg ccgtgaaatc
    34561 aggttaatgt cgtgcgttgc catcaatacg gttaccccaa cgcggttaaa ctcttcaaac
    34621 agacgtagaa tcccttccga caacgcgtcg tccaggttac cggtcggttc gtccgccagc
    34681 agtaccgcgg gcttattcac caccgcgcgg gcaatgccaa cacgctgttg ttcaccgccg
    34741 gaaagctgaa tagggaagtt cttcgctttg tccagtagcc cgactttatc cagtgccgcc
    34801 gacacccggc gacgaatatc gtcaccgctg gcaccagcga taatcagcgg gatcgccacg
    34861 ttatcgtaga cagtacggtc catcagtaga tggtgatcct ggaaaatcat gccaatctgg
    34921 cggcgcagaa acggaacttc acggtttttc agacgcgtga tgtcatggcc gctaaaccag
    34981 attttcccgg cgctgggccg ctcaatccca cagatcagct tcaggagggt acttttccct
    35041 gcgccggaat gaccggtcag aaacgccatc tcacccggct gcatatggaa cgtaacgccc
    35101 tgcagcgcct gtctcccacc gagataagcc ttgctgacat gttcaaagcg aatcattgtt
    35161 aatcctctcg ggcaaaaagt gcctctataa agtcgtccgc cttaaacgga cgcaaatcct
    35221 caatacgttc gccgacacca atgtagcgga tagggatacc aaactggtca gccaccgaga
    35281 aaattacccc gcctttcgcc gtgccgtcca gtttcgttag cgtgatgccg gttaagccaa
    35341 cagcttcgtg gaacagtttg gcctggctta ccgcgttctg cccggtgctg gcatcaatag
    35401 tcagcataac ttcatgcggc gcttcaacgt cgagtttctt catcacgcgg acgattttct
    35461 tcaactcttc catcagatgc gatttgttct gcagacgtcc ggcggtatcg gcaatcagga
    35521 cgtcgatatt acgcgcttta gctgcctgaa tggcgtcgaa gataacagag gcggaatccg
    35581 caccggtatg ctgggcaatc accggaatat tgttgcgctg accccagacc tgaagctgtt
    35641 caaccgcagc tgcacggaaa gtatcgcccg ccgccagcat caccgattta ccctgctgct
    35701 caaactgacg cgccagctta ccaatcgtcg tggttttacc cacaccgttg acgcccacca
    35761 tcaggatcac gaacggcgtt ttatcttcaa cattcagcgg ctcatcgact ttcgccagaa
    35821 tctcacccat ctcttctttc agcaggccat agagcgcctc ggcatcacga agctgcttgc
    35881 gggatgcgcc ttccgtcaga ttggtgataa ttttacgtgt ggtttccacg cccacatcgg
    35941 cgatcaacag ctgttcttcc agctcttcaa acagatcatc gtcgattttt ttaccgcgga
    36001 acaggctgat aaatccggaa ccgagatttt ctttggtttt taacaggctg cgtttcaggc
    36061 gcgcgaagaa gccttctttg gtcggttttt cttgctcctg ggcaatttct tcaaccggct
    36121 gttcttcttc tgccggagga actaccatca ccgcctcttc tgccgcttcg gcagccagcg
    36181 cctgtgcttc cagctcttcg tcggtgattt cttctttagc cgcttcttct tctgccgctt
    36241 cgacaatctc tacggtttcc gcttcagcct gccactcttc tggcgaaacc tcttcggcgt
    36301 tgacgtcttc cggcaacggc agctcttcac gttcgatagc cactggctcc ggcgtttctt
    36361 ctacgaccgg ttccggctgt gcaacaactt ccgcttcagg ctgcgctttt tcactttcaa
    36421 caacctgttc agtgacttcc acaacgtcgg cagcaaaagt ttccgcctcg gcttcagtat
    36481 gcgcctgcgg ctgctcttca acggcttgtt cagaagcctt cgcaggctct tgcgcctgaa
    36541 cgatttcttc tacaaccggt tgttcattct gaacttctgt ctctttttcc ggggtctgct
    36601 ctttttgacc aaagcccagc caggaaaaaa agccacgtct tttttctttc gccatttgcg
    36661 actacactcc tcgctgttgc ttcatggcac agcgttaacg ctatgtacat agcagctaaa
    36721 aaaatgatga aatagtctat cacttaactt aattcacatc accgcctgca agtatgtgtt
    36781 atctggcgga ttgagcaatt tatcatgaaa aaaccgaatc attccggcag cggccaaatc
    36841 cgcattattg gcgggcagtg gcgaggccgt aaactcccgg ttcctgatag cccaggtctg
    36901 cgccccacca ccgaccgcgt acgcgaaacg ttgtttaact ggctggctcc ggtcattgtt
    36961 gacgcccaat gtctggattg cttcgccggg agcggcgcgc tggggctgga agcgttatcg
    37021 cgctacgctg cgggggcaac gttgattgag atggatcgcg tggtttctca gcagttaatt
    37081 aagaatctgg cgacactaaa agcaggcaat gcacgcgtgg tgaacagcaa cgcaatgtca
    37141 ttcctggcgc aaaaaggcac accgcataat atcgtgtttg tcgatccacc gttccgccgt
    37201 ggcttgttag aagagacgat aaatttactg gaagataacg gctggctggc tgacgaagcc
    37261 ctgatttatg tcgaaagcga agtcgaaaac ggtctgccca ctgttccggc aaactggtca
    37321 ttacatcggg aaaaagtggc gggtcaggtg gcttatcggc tgtatcaaca cgaagcacaa
    37381 ggagaaagtg atgctgatta atattggtcg tttgttaatg ctctgcgttt ggggattttt
    37441 aatcctcaat ctggtgcatc ccttcccgcg cccgctaaat attttcgtta acgtggcgct
    37501 gatttttacc gtgctgatgc atggtatgca gctggcgcta ttgaaatcca ctttaccgaa
    37561 agatggcccg cagatgacca ccgccgaaaa ggtgcggatt ttccttttcg gcgtgtttga
    37621 actgctggtc tggcagaaga aatttaaagt taaaaaataa ccttattgtt cgccaacaaa
    37681 gctaacaaag cgcgttcctt tatagctcag cgtaccttta tcgcccacgg tcagggcgtg
    37741 gtactgctgg gcgtcgagac gaaacgtctg ctccattcct ccgctttgcg gtttgaagct
    37801 tgcctcatag cgcatactgg tgcctgccgg agtcacttcc tgctggcgcg aacggcgatc
    37861 gttaatcggt ttttcccgct tgttgctcac caccaccagc ttttgctgga gcggagccat
    37921 atcattatca gctttttccc gtcgctgttg cataaaacga aacgatgcgg cgacgacaat
    37981 taagccaatg ataacaataa agaaaagagg tggtttactc atctttatcc ctcatcggaa
    38041 aatgcggaat taagcatacc ctgccagtta tggtgttgtc atccgtccac cctcgccact
    38101 aaactggaag caagaccgta ggcattccgc ttacgaaaaa ataacgaatt caaggaacta
    38161 agatgctttg gtcgtttatc gctgtctgtc tttccgcatg gctatctgtg gatgcatcgt
    38221 atcgtgggcc aacctggcaa cgctgggtgt ttaaaccgtt aacccttctt ctcctgctgt
    38281 tactggcctg gcaagcaccg atgttcgacg ccattagcta tctggtgctg gcgggactgt
    38341 gcgcctcact gctgggcgat gcgctaaccc tgttgccacg tcaacgtctg atgtacgcca
    38401 tcggcgcgtt tttcctctcg cacctgctgt acaccatcta tttcgccagt cagatgacgc
    38461 tctctttctt ctggcctctg ccactggtgt tgctggttct gggtgcgctg ctactggcga
    38521 ttatctggac gcggctggaa gagtaccgtt ggcctatctg cacgtttatc ggcatgacgc
    38581 tggtgatggt gtggctggca ggtgaactgt ggttcttccg tccgaccgct ccggcgctct
    38641 ctgcgtttgt tggcgcttcg ttgctgttta tcagtaactt tgtctggctg gggagccact
    38701 atcgccgacg cttccgtgcg gataacgcga ttgctgcggc ctgctacttt gccggtcact
    38761 tcctcatcgt ccgctcactg tatctctgat aaaacttgac tctggagtcg actccagagt
    38821 gtatccttcg gttaatgaga aaaaacttaa ccggaggatg ccatgtcgac acctgacaat
    38881 cacggcaaga aagcccctca atttgctgcg ttcaaaccga taaccacggt acagaacgcc
    38941 aacgactgtt gctgcgacgg cgcatgctcc agctcgccaa cgctctctga aaacgtctcc
    39001 ggcacccgct atagctggaa agtcagcggc atggactgcg ccgcctgtgc gcgcaaggta
    39061 gaaaatgccg tgcgccagct tgcaggcgtg aatcaggtgc aggtgttgtt cgccaccgaa
    39121 aaactggtgg ttgatgccga caatgacatc cgtgcccagg ttgaatctgc ggtgcaaaaa
    39181 gcgggctatt ccctgcgcga tgagcaggcc gccgacgagc cacaagcatc gcgcctgaaa
    39241 gagaatctgc cgctgattac gcttatcgtg atgatggcaa tcagctgggg tctggagcag
    39301 ttcaatcatc cgttcgggca actggcgttt atcgcgacca cgctagttgg gctgtacccg
    39361 attgctcatc aggcattacg gctgatcaaa tccggcagct acttcgccat tgaaacctta
    39421 atgagcgtag ccgctattgg tgcactgttt attggcgcaa cggctgaagc tgcgatggtg
    39481 ttgctgctgt ttttgattgg tgaacgactg gaaggctggg ccgccagccg cgcgcgtcag
    39541 ggcgttagcg cgttaatggc gctgaaacca gaaaccgcca cgcgcctgcg taacggtgag
    39601 cgggaagagg tggcgattaa cagcctgcgg cctggcgatg tgattgaagt cgccgcaggt
    39661 gggcgtttgc ctgccgacgg taaactgctc tcaccgtttg ccagttttga tgaaagcgcc
    39721 ctaaccggag aatccattcc ggtggagcgc gcgacgggcg ataaagttcc tgcaggagcc
    39781 accagcgtag accgtctggt aacgctggaa gtgctgtcag aaccgggtgc cagcgccatt
    39841 gaccggattc tgaaactgat cgaagaagcc gaagagcgcc gcgcccccat tgagcggttt
    39901 atcgaccgtt tcagccgtat ttatacgcca gcgattatgg ccgtcgccct gctggtaacg
    39961 ctagtgccgc cgctgctgtt tgccgccagc tggcaggagt ggatttataa aggtctgacg
    40021 ctgctgctga ttggttgccc gtgtgcgtta gttatctcaa cgcctgcggc gataacctcc
    40081 gggctggcag cagcagcgcg tcgtggggcg ttaattaaag gcggtgcggc gctggaacaa
    40141 ctaggtcgtg ttacccaagt ggcgtttgat aaaaccggta cgctgaccgt cggtaaaccg
    40201 cgcgttaccg cgattcatcc ggcaacgggt attagtgaat ctgaactgct gacactggcg
    40261 gcggcggtcg agcaaggcgc gacgcatcca ctggcgcaag ccatcgtacg cgaagcacag
    40321 gttgctgaac tcgccattcc caccgccgaa tcacagcggg cactggtcgg gtctggcatt
    40381 gaagcgcagg ttaatggtga gcgcgtgttg atatgcgctg ccgggaaaca tcccgctgat
    40441 gcatttgctg gtttgattaa tgaactggaa agcgccgggc aaacggttgt gctggtagta
    40501 cgtaatgatg acgtgctggg tatcattgca ttgcaggata ccctgcgcgc cgacgctaca
    40561 actgccatca gtgaactgaa cgcgctgggg gttaaaggag tgatcctcac cggcgataat
    40621 ccacgcgcag cggcggcaat tgccggggag ctggggctgg agtttaaagc gggcctattg
    40681 ccggaagata aagtcaaagc ggtgaccaag ctgaatcaac atgcgcccct ggcgatggtc
    40741 ggtgatggta ttaacgacgc gccagcgatg aaagctgccg ccatcgggat tgctatgggt
    40801 agcggcacag acgtggcgct ggaaaccgcc gacgcagcat taacccataa ccacctgcgc
    40861 ggcctggtgc aaatgattga actggcacgc gccactcacg ccaatatccg ccagaacatc
    40921 accattgcgc tgggactgaa agggatcttc ctcgtcacca cgcttttagg gatgaccggg
    40981 ttgtggctgg cagtgctggc agatacgggt gcgacggtgc tggtgacggc gaatgcgtta
    41041 aggttgttgc gcaggagata aggcaaaccg atcgcaacat tgagcgcgat cggtcccctc
    41101 gcccctctgg ggagagggtt agggggtgag gaaaaggcgg catcgaagcc aatcagcccc
    41161 tatcaaccgc ctttacgaat caaataacga taaggcagtc catccgtctc tttcgcaacc
    41221 agttcgtgtt ccataaaggt acaaaaccca ggaatatcgc gggtagtggc cggatcgtcg
    41281 gcgataatca gcaacgtttc gccaggctgc atattgcgca cggttttgcg caccatcatc
    41341 accggttccg ggcagcgcag gccaagcgcg tcgagtgtgt ggtcagggct ggaaaagaga
    41401 tcggtcattt tcttctcatc acttaaaaaa acggcgctag tttacgccct gtgagtccgt
    41461 aagcaaacca ggttaacgat tgcgtgaaaa ttaaccattg cattgtcaac gtaaagcagt
    41521 atcatgcggc ggctcgaaaa aagggtaagc acgttattat gttaaggtaa cagacgtgtc
    41581 gtacgtattg ggttccctca ccccaatggt taatcaaaaa ggtacaatat gaacgttttc
    41641 tcgcaaactc aacgctataa ggccttgttc tggttatcgt tatttcatct tctggtgatc
    41701 acctccagta actatctggt tcagcttccc gtctccattt ttggttttca taccacctgg
    41761 ggcgcgttta gctttccgtt tatttttctt gctaccgacc tgaccgtgcg tatttttggc
    41821 gcaccgctgg cccgacgcat tatcttcgcg gtaatgatcc ctgcgttatt aatctcctac
    41881 gtcatctcgt cgctattcta tatgggttcc tggcagggat tcggcgcact cgcccacttc
    41941 aacctgtttg tcgcccgtat cgccaccgcc agtttcatgg cctacgcgct ggggcaaatc
    42001 ctcgacgtgc acgtttttaa ccgcctgcgt cagagtcgcc gctggtggct ggcaccgaca
    42061 gcgtccacac tgttcggtaa cgtcagcgac acgctggcct ttttcttcat tgccttctgg
    42121 cgtagcccgg atgcctttat ggctgaacac tggatggaaa tcgcgctggt cgattactgt
    42181 ttcaaagtgt taatcagtat cgttttcttc ctgccaatgt atggcgtatt actcaatatg
    42241 ctgttgaaaa gactggcaga taaatccgaa atcaacgctt tgcaggcgag ttaaaggttc
    42301 gttatcagag ttgtgataag atggatgaat gagccgttat ggccgtttat cgaaaggaag
    42361 aagtcaatgc gcaatctggt taaatatgtc ggaattggcc tgctggttat ggggcttgcg
    42421 gcctgtgatg ataaagacac taacgctacg gcgcagggtt cggtcgcgga aagtaacgct
    42481 accgggaatc ccgtcaacct gcttgatggc aagttaagtt tctcgctgcc agcggatatg
    42541 accgaccaga gcggtaagct gggaacgcag gccaataaca tgcatgtctg gtccgacgcc
    42601 accgggcaga aagcagtcat cgtcatcatg ggcgatgatc cgaaagaaga tctggcggtg
    42661 ttggcgaagc gtctggaaga tcagcaacgt agccgcgatc cgcagctgca agtggtaacc
    42721 aataaagcca ttgagctgaa aggtcacaaa atgcagcagt tagacagtat tatctccgcg
    42781 aaaggccaga cggcgtactc ttccgttatt ctgggtaacg tgggtaatca actgctgacc
    42841 atgcaaatta cgctgccagc tgacgatcag caaaaagcgc aaaccaccgc agaaaacatc
    42901 attaatacgc tggttattca gtaagtttta agatgatgag gcggcctcag ggacgtgttc
    42961 cggaggccgt ttttttaatc gccacgtcag taataacgcg attgcgacca gtcccgccgc
    43021 cgccagataa atcaccggta cgcccgccca gctcatcacc agcccagcca gtggtccagt
    43081 cacgccaagc gataaatcca taaatacggt gtaagttgcc agcgccgccc cctaattttg
    43141 ctgcggaacc gcttttaccg ccactacacc caatgccggg aacaccagcg aaaacccggc
    43201 ccccgccagt aagacgccga ttttcgccat ccacggcata gtcgccacgc caaccagtag
    43261 caggccgatt atctcaacgc taaagcaaat catcgctacg tttaagccac cgatacggtt
    43321 aatgccgtta gggaataaca aacgcgtacc gacaaacgca cagctaaaca gcgtcagcgc
    43381 gaaagccgca ccgtcccaac ctttagcgtc ataaaacagc gtgataaagg tggcgatgac
    43441 gccaaatccg gcggaagcca gtgccagcgc cataccgtac agccagacgc gcccaagcac
    43501 cgcgcgaaac ggcagcggtt tgcctttact ggcttttacc gtcggacgcg ggatcgccaa
    43561 caaaatggcc accagcgcca cgcccataat gattaacgct aacgcctgca agccgcccca
    43621 gtgataaaac acgacgccta acggcgcacc catcgccatc gccccgtaag tgacaatgcc
    43681 gttccacgaa atcacccgcc cgatatgcag cgagccaacc actccaacgc cccatagggt
    43741 cgatcccgtt ccggcaaaac tttgcccaat cccaaggatg acgcgcccca ggcaaagtaa
    43801 taacaggctg atgacaggca gactggcggt taatcctgcc gtcagatacc ccagaccgct
    43861 caaaaagcag ccgcataaac cgaagacgac aatctttttg ggtcccagca aatcggcgta
    43921 acgtccggca tgagggcggc tcagcaaggt ggcgaaatat tgcaggctga taaccaatcc
    43981 tgcccagaag gcgctaaagc ccatcacatc atggacatag cccggtaata cagcgagcgg
    44041 caacccgatg gtgaggtagc tggcgaagtt aaacatgaca atggagacaa tgcgcaaatt
    44101 caggcgcaat ccgtttagcg cgggttcggc tacgggttcg ggcatgagga tcaccacatt
    44161 tttacaacag tgtttcattt ttaccatgtg ctgacgtgaa aatcagcagt aagaatcaga
    44221 atattgctgg cgtgactccc gctacactta acacaaaaag tcacaaggaa gccccaatgg
    44281 aaacccctca acccgataaa acgggcatgc acattctgct caagttggcc tcgctggtag
    44341 tgatcctcgc gggcattcac gcagcggcag atatcattgt gcagctgtta ctggcgctgt
    44401 tttttgccat cgtcctcaac ccgctcgtta cctggtttat tcgtcgggga gtacaacgcc
    44461 ccgttgccat tacgattgta gtggtggtga tgctgatcgc actaaccgcg ctggtcggcg
    44521 tactggcggc atcgtttaac gaatttatct ctatgctgcc gaagtttaat aaggagctga
    44581 cgcgcaaact ttttaaattg caggagatgt tgccttttct taatttgcat atgtcgccgg
    44641 agcgaatgct gcagcggatg gactcggaaa aagtggttac cttcaccaca gcgctaatga
    44701 ccgggctttc cggggcaatg gcgagcgtgc ttttgctggt gatgaccgta gtttttatgc
    44761 tgtttgaagt gcgccacgtc ccttacaaaa tgcgttttgc gctgaataac ccacagattc
    44821 acatcgcagg attacaccgc gcacttaaag gcgtttcgca ttatctggcg ttaaagacgc
    44881 tactcagttt atggacaggt gtcatcgtct ggctggggct ggcgctaatg ggcgtacagt
    44941 ttgcgctgat gtgggcagta ctggcgtttt tgctcaacta cgtgcccaat atcggcgcgg
    45001 taatttccgc cgtaccgcca atgattcagg tgctgctgtt taatggtgtt tacgaatgta
    45061 ttctggtcgg cgcattgttt ttagtggtcc atatggtcat cggcaatatt ttagaaccac
    45121 ggatgatggg ccatcgcctg gggatgtcca ccatggtggt atttctttca ttgttaatct
    45181 ggggatggct gctcggcccg gtagggatgc tactttcggt gccattaacc agagtgtgta
    45241 aaatctggat ggaaacgacg aaaggtggca gcaaactggc gattttactg gggccggaca
    45301 gaccgaaaag tcggttaccg ggatgaggcg acaagtgata cgatacgcac tttcattttc
    45361 cattaaacgt tggccctgat atgtatcgga tagttctggg gaaagtttcg accttaagcg
    45421 cagctccact gccaccgggt ttacgcgagc aagcaccgca aggtccacga cgcgaacgct
    45481 ggctggcggg gcgtgcattg ctttcgcaca cgctttcccc gctaccggag atcatctatg
    45541 gagaacaagg caaacctgca ttcgcgccgg aaacgccgct atggttcaac ttaagccata
    45601 gcggtgacga tatcgctctg ctgttgagcg atgaaggcga agtcggctgc gatatcgaag
    45661 tgattcgccc gcgcgccaac tggcgctggc tggcgaacgc cgtgttcagc ctcggggaac
    45721 acgctgaaat ggacgccgtg catcctgatc agcaactgga aatgttctgg cgcatctgga
    45781 cgcgcaaaga agccatcgtt aaacagcgtg gcggcagcgc ctggcaaatc gtcagcgtag
    45841 atagcaccta tcactcctcg ctgtcagtca gccattgtca gctagaaaat ttaagccttg
    45901 cgatctgcac ccccaccccc tttacgctca ccgccgacag tgtgcaatgg atcgattcag
    45961 ttaactgatc cgcccacccg actgcccatc tattgatcca gaacaggtaa tcagtatgac
    46021 gaatacttaa aatcgtcata cttatttccg ccatctattt taatccattg gggttaccat
    46081 gctctccaca ctccgccgca ctctatttgc gctgctggct tgtgcgtctt ttatcgtcca
    46141 tgccgctgca ccagatgaaa tcaccaccgc ctggccggtg aatgtcgggc cactaaaccc
    46201 gcacctttac acgcctaacc agatgttcgc ccagagcatg gtttatgaac cattggtgaa
    46261 atatcaggca gacggttcgg tgatcccgtg gctggcaaaa agctggactc attcagaaga
    46321 tggtaaaacc tggaccttca ccctgcgtga tgacgtgaaa ttctccaacg gtgaaccgtt
    46381 cgatgccgag gcggcggcag aaaacttccg cgcagtgctc gataaccgtc aacgtcacgc
    46441 ctggctggag ctggcaaacc agattgttga tgttaaagca ctcaataaaa cagagctgca
    46501 aattaccctg aaaagcgcct actatccttt cctgcaagaa ctggccctgc cccgtccttt
    46561 ccgttttatc gctccctcgc agtttaaaaa ccatgaaacc atgaacggaa ttaaagcgcc
    46621 gattggcacc ggaccgtgga ttttgcagga atcgaaactg aatcagtacg atgtcttcgt
    46681 ccgtaacgaa aactactggg gcgaaaagcc agcgattaaa aagatcacct ttaacgtcat
    46741 cccggacccg actacccgcg cggtggcgtt tgaaactggc gatatcgacc tgctgtacgg
    46801 aaacgaaggg ttattaccgc tcgatacctt cgcccgcttt agccagaatc cggcttacca
    46861 cacccaactg tcacagccgc tcgaaaccgt gatgctggcg ctcaataccg ccaaagcccc
    46921 caccaacgag ctggcagtac gtgaagctct taattacgcg gtaaacaaaa aatcgctgat
    46981 tgataacgcg ttatatggca cccagcaggt cgccgacacc ctgtttgccc cttctgtgcc
    47041 ttacgctaat ctcggcctga aaccgcgcca gtacgatccg cagaaagcga aagagttgct
    47101 ggaaaaagcc ggttggacgc tgcctgcggg caaagacatc cgcgagaaaa atggtcagcc
    47161 gctgcgcatt gaactgtcgt tcatcggcac cgatgcgtta agcaaatcga tggcggaaat
    47221 cattcaggct gatatgcgcc agattggcgc agatgtctcg ctgattggcg aagaagagag
    47281 cagtatctat gctcgtcagc gcgacggtcg ttttggcatg attttccacc gcacctgggg
    47341 cgcgccatac gatccacacg ccttcctgag ttcaatgcgc gtaccgtcgc acgctgactt
    47401 ccaggcacag caaggattag ccgacaagcc gctgattgat aaagagatcg gcgaagtgct
    47461 ggcgacccat gacgaaacgc aacgtcaggc gctgtatcgc gacattctga cccgtctgca
    47521 tgacgaggcg gtttatctgc ctatcagcta catctcgatg atggtggtat caaaaccgga
    47581 gctgggtaat atcccctacg cgccgatcgc caccgaaatt ccgttcgaac agattaaacc
    47641 ggtgaaacct taatgttgcg ttacgtatta cgccgctttc tgctgctgat cccgatggtg
    47701 ctcgccgcct cggtgatcat ttttctgatg ctgcgcctcg gtaccggcga cccggcgctc
    47761 gattatctgc gtttatctaa cctgccgccg acgccggaga tgctggcctc tacccgcacc
    47821 atgctcgggc tggatcagcc gctgtacgtc cagtacggta cctggttgtg gaaggcgctg
    47881 catcttgact ttggtatctc attcgccagc caacgcccgg tactggacga tatgctgaac
    47941 ttcctgccca ccacgctgga acttgcaggt gcggcgctgg tattaattct gctcacttcc
    48001 gtaccgctcg gtatctgggc ggcgcgccac cgcgatcgtt tgccggattt cgccgtgcgc
    48061 tttatcgcgt ttcttggcgt gtcgatgccc aacttctggc tggcattttt gctagtgatg
    48121 gcgttttcgg tgtatctgca atggctaccc gcgatgggct acggcagctg gcagcacatc
    48181 attttacctg cggtttccat cgcctttatg tcgctggcga ttaacgcgcg actgctgcgc
    48241 gccagtatgc tggacgtcgc cggtcagcgt cacgtcacct gggcgcggct acgcggcctg
    48301 aatgacaaac agaccgaacg ccgccatatt ctgcgcaatg cctcgctgcc gatgatcacc
    48361 gccgtgggga tgcacattgg cgaattgatt ggcgggacga tgattatcga aaacatcttt
    48421 gcctggcctg gcgtcgggcg ctatgcggtg tcggcgattt ttaaccgtga ctatccggtg
    48481 attcagtgct ttacgctgat gatggtggtg gtttttgtgg tctgtaattt gattgtcgat
    48541 ttgctcaacg ccgcgctgga cccgcgcatt cgtcgtcatg aaggagcgca cgcgtgaact
    48601 ttttcctctc ttcccgctgg tcggtacgcc tggcgctgat cattatcgcc ctgctggcgc
    48661 tgattgcgct caccagccag tggtggctgc cgtacgatcc acaggcaatt gatttgccgt
    48721 cgtgcctgct ttcgccggat gcgcagcact ggctgggcac cgatcactta ggtcgcgata
    48781 ttttctcgcg gctgatggca gcgacccgcg tgtcgctcgg ttcggtaatg gcctgcctgc
    48841 tgctggtgct gacattaggg ctggttattg gcggcagcgc cgggttgatt ggcgggcgcg
    48901 ttgatcaggc caccatgcgc gtcgccgata tgtttatgac cttcccgacc tcgattctgt
    48961 cgttctttat ggttggcgtg ctcggcaccg ggctgaccaa cgtaattatc gccatcgccc
    49021 tgtcgcactg ggcgtggtat gcacgcatgg tgcgcagctt ggtgatttca ctacgccaac
    49081 gcgagtttgt gctggcgtca cggctttccg gtgcgggcca tgtgcgggtg tttgtcgatc
    49141 atctggcagg cgcggtgatc ccttcgctgc tggtgctggc aacgctggat atcggccata
    49201 tgatgctgca cgtcgcgggg atgtctttcc ttggcctcgg tgtgaccgcg ccgaccgccg
    49261 aatggggcgt gatgattaac gacgcgcgcc agtatatctg gacccagccg ctgcaaatgt
    49321 tctggccggg gctggcgctg tttatcagcg tgatggcctt taacctggtg ggtgacgcac
    49381 tgcgcgatca tctggatcct catctggtga cggagcacgc acactaatgc cgcaacagat
    49441 tgaactacgt aatatcgcgc tacaggccgc gcagccgctg gtacacggtg tatcgttaac
    49501 cctgcaacgc gggcgcgtgc tggcgttagt cggcggtagc ggcagcggga aatcattaac
    49561 ctgcgccgcg acgctgggca ttttgcccgc tggcgttcgc cagacggcgg gggaaatttt
    49621 agccgatggc aaaccggttt cgccttacgc cctgcgcggc atcaaaattg ccaccatcat
    49681 gcagaacccg cgcagcgcct ttaatccact gcacaccatg cacacccacg cgcgggaaac
    49741 ctgcctggcg ttagggaaac ccgccgatga cgctacgctt accgctgcca tagaagcggt
    49801 ggggctggaa aacgccgcgc gcgtgctgaa gctgtacccg ttcgagatga gcggcggcat
    49861 gttgcagcgc atgatgattg cgatggcggt gctgtgtgaa tcaccgttta tcatcgccga
    49921 tgaaccgacc accgacctcg acgtggtagc acaggcgcgc atcctcgatc tgctggaaag
    49981 cattatgcaa aaacaagcgc cgggaatgct gctggtcacc catgatatgg gcgttgtggc
    50041 gcgtctggcg gatgacgtgg cggtgatgtc acaaggtaaa attgtcgaac agggcgatgt
    50101 agaaacgctg tttaacgccc ccaaacatac ggtgacgcgc agcctggttt ccgctcatct
    50161 cgccctctac ggtatggagc tggcatcatg accttactta acgtctgcgg cctttcccat
    50221 cactatgccc acggtggatt tagcggaaaa catcaacatc aggcggtgct gaataacgtt
    50281 tccctgaccc tgaaaagcgg cgaaaccgtc gccctgctgg gacgcagcgg ctgcggaaaa
    50341 agcaccctcg cgcggctgct ggtaggctta gagtctccct cacaagggaa tattagctgg
    50401 cgcggcgaat cgctggcgaa acttaatcgc gcccaacgta aagccttccg ccgtgatatt
    50461 cagatggtgt ttcaggactc catcagtgcc gtgaatccgc gcaaaaccgt gcgcgagatc
    50521 ctgcgtgaac cgatgcgcca cctgctatca ctgaaaaaat ccgaacaact ggcgcgcgcc
    50581 agcgaaatgc tgaaggcggt cgatctcgat gacagcgttc tcgacaaacg cccaccgcag
    50641 ttaagcggcg gccagctcca gctcgtctgc ctggcacgcg cgctggcggt cgaaccgaaa
    50701 ttgctgattc tggatgaagc cgtttctaac cttgatctcg tgttacaggc gggcgtcatt
    50761 cgcctgctga aaaatctaca acaacagttt ggcactgcct gcctgttcat cactcatgac
    50821 ttacgcctgg tggaacgctt ttgccagcga gtgatggtga tggacaacgg acaaatcgcc
    50881 gaaacccagg cggtgggaga taaattaacc ttttcctctg acgccggacg tgtgctacaa
    50941 aacgcggtat tacccgcatt ccccgtgcgc cgtcgcacct cagaaaaggt ttaacgccaa
    51001 tgcaacgagt caccatcacg cttgatgacg atttactgga gacgctggac agcctgagcc
    51061 agcgtcgtgg ttataacaac cgttccgaag ctatccgcga tattctgcgt agtgccctgg
    51121 cgcaggaagc tacccagcag cacggcacac aaggtttcgc ggtgctgtcg tatgtgtatg
    51181 aacacgaaaa acgcgactta gccagccgta ttgtttccac ccagcatcat caccacgacc
    51241 tctccgtcgc cacgctacat gtgcacatca accacgacga ctgtctggaa atcgccgtgt
    51301 tgaaaggtga catgggtgac gtgcagcatt ttgccgatga cgttatcgcc cagcgcggcg
    51361 tgcggcacgg gcatttgcag tgcctgccga aggaagattg agttatttct aaaacaagga
    51421 aagaaaagat attaactgaa cttaatttat aaaattaagt tcagttgcat agatagatcc
    51481 tgtaaaggca gatattctgc gtaatgtacg tcgctaaaat ttataaatga ttcaaacgct
    51541 cattcagtgt ttcaataata taggtattaa cagagacttg ttgctgcgca gccgcattat
    51601 taaggcgttc actcagcgat tctggatagc gtaacgtaaa ggtcttaatc ttttctgtac
    51661 gcgcatacgg ctcaataccc gcagctttac aatcctcaag atactctcgc agggaaagct
    51721 ctccttcttt ttgcagcccc tgaatactgt cagagacaaa atcacaataa cctgacaagc
    51781 ccaggaattt gccacgaaat gcattcagtt ctggcacgta ggtaataaca gctggttgcc
    51841 cggcaatctc catagagttt ggcgtcttca gtttgatcat ggttttaccc ctatacttag
    51901 taaccagtca cgcacgcttt ctacagcgcc tttatcggta tctggcgacg gatgaggccg
    51961 atggaaacac gcaacgctca tatttagtat gaacttgcaa cgcgaacctc ttccttcctt
    52021 aatttctccg cctaatgctt taaccagtga ctctatatca gaccacttga tcccttgagg
    52081 aacaggtgtt ttaaatattt gatccaatgt atttttttgt ttcttccgta aagcctgtac
    52141 cttggttctc atggcatgtc cttatgactt cactttatga catcacagta tgaagtcaag
    52201 tttcccttcc ttacgccatt gtcccaatcg tcttcctgaa tcgcagcagt gcgatggtga
    52261 aaaacgcgcc gccaattgcc atcagcgtca ggaactgcgg ccagacgatt tcgaatccgg
    52321 caccccggta gaggatggcc tgcgcgaggc taacaaagtg tgtcgtcggc atggtcagca
    52381 taatgtcctg caccatctgc ggcatacttt cgcgcggcgt ggatccaccg gaaagcattt
    52441 gcagcggcag cagcaccaga atcaccagca gccccagttg cggcattgaa cgcgctatcg
    52501 tccccataaa aatgccgatt gacgtggtgg caaacagact gagcgccacg cccagcataa
    52561 acagcgggat cgagccttca atcggtacgc ccagtacacc tttcaccatc agcaccagcg
    52621 ataatcccga taccaccagc accaccagcc ccatcgacca gatcttcgcc atcatgatct
    52681 caaacggcgt tatcggcatc accagtaaat gttccaccgt gccgtgttca cgctcgcgga
    52741 tcagcgccga tccggtcaat acaatcgcca gcatggtaat gttgttgatg atcgccatca
    52801 ccccgccaaa ccacgcggga tcaaggttcg ggttaaagcg catccgggtt tccagcgata
    52861 ccaacggttc gctgttatca cggtagcgcg cgacaaagct gttcacttca ccgttgataa
    52921 tattctggat atacccattg ccggtaaatg cctggctcat gcgcgtggca tcgacgttca
    52981 cctgaatatc cggctggcgt ccggcgagga catcacgctg aaaattaggc ggaatattta
    53041 tcgcgaaggt atagcgtccg gcgtccagtc cggcatccat ctcatcggcg gtgatcatct
    53101 ccggtggcaa aaaccacgga cgatagaagc tgttaacgat ccggttcgat aactgcgatt
    53161 gatccatatc ggcaatggcg atcggcgcga ggttcaacga tcctggcgta acggtcgctg
    53221 acgaatacac cgacaccgta aacgagaaga caatcagcgt cagcatcgct ttatcaccga
    53281 gcagactgcg caactctttg atacccagat taaaaatatt gcgtaaatgg cgcatcatcc
    53341 ctcctgtttt ttcagcagca ggatgcttaa gcccatcacc agcgggatgg ctatcagcaa
    53401 cgggataaaa agttgccaca aatcagtcag atccagcgct ttcgagaacg ttccgcgggc
    53461 gatagtcaga aaatgactgg tcgggtaaac ctcgccgatc caacgtccag gcccttccag
    53521 cgaagctacc ggatcgatca tcccggaaaa ctgtgtcgcc gggatcaatg tgataatcgc
    53581 cgttccgaaa atggcggcaa tctggctttt cataaaggtg gagatcagca gccccattcc
    53641 agtggcaatg atgatataca gcagcgccgc caaggtgagc gtcaggaaac tgcctttatg
    53701 cggtacgcca aacacaaaca ccgacaggcc gcagagcagg aaaaagttca gcatccccag
    53761 cgcgatgtac ggcagctgtt tgccaagcaa aaattcgcta cgggtggtgg gggtcacgta
    53821 aaggttgata atcgacccta gctctttttc ccgcaccacg ctaagggcgc ttagcattga
    53881 cgggatcatc atcagcagaa gcgggatcac cgccggaaca atcgctggca ggctttttac
    53941 gtccgggtta tagcgataac gcgtctcaat gttcatcagc ccgctttggc tggcgggtgt
    54001 caattgtcgg ctcgccacat cctgaagcca actctggtgc attgcctgca cgtaaccttt
    54061 taccgtttcg gcacgactgg gcatcgctcc gtcgatccag acgccgagtt ccacaggcgt
    54121 accacgcgcg atatcgcgcc cgaaattggg cgggatctca atggccaccg tgatatcgcc
    54181 cgcacgcatc cgacgatcaa gctcgtcata actggtgagc ggcggctgtt cgataaagta
    54241 acgggaaccg gagaggttga gtgtccacgc ctggctactg acggtctggt cgcggtcgag
    54301 caccgcaaag cgcaggtttt ccacatccat actgatgccg taacccatta tcagcatcag
    54361 gatcaccgtt cccatcagcg ccagcgtcga acgtactgga tcgcgtcgca gttccagcgc
    54421 ttcgcggcgg ctgtagctaa acagacggcg caggctaaat ccctgacgcg gcgcgtgggt
    54481 ggtgtcgtgt accacgggcg gcgcttcggc ttcgttgctc tgccctgccg cttcctgcaa
    54541 ataggcgata aatgcctctt ccagactggc ggctccgcgt ttctcaacca gttcctgcgg
    54601 tgtaccgctg gcaagcactt ttccggcgtg catcagtgag atgcggtcgc aacgttccgc
    54661 ttcgttcata aagtgggtgg agatgaagat agtcactttg tcctggcgcg agagatcgac
    54721 catcaactgc cagaacatat ccctcgccac cggatcgaca ccagaagtag gctcatcgag
    54781 gattaacatc tccgggcgat gaatcaccgc caccgccagc gaaagccgct ggcgaatgcc
    54841 gagcggcaat gactccggca gaacatcttc aacgtcgttg agcttaaaac gctcgctcat
    54901 ttcagccact cgcgcgggaa tttccgcttc cgggatgtga aacaaacggg catgtaactc
    54961 caggttttgc cgcacggtga gttcgttata gagcgaaaac gactgcgaca tatagcccac
    55021 acgacggcgg gtatcgatat cttttggatc aaccggttgc ccaaatagcc acgcctcacc
    55081 ttcgctggcg ggcagcagtc cggtaagcat tttcatggtg gtggatttac cgcagccgtt
    55141 cgaaccaagg aatccaaaaa tctccccgcg tggaatgcgg aaattgacgt gatcaacggc
    55201 aacgaaggaa ccaaaacgca tggtcagatc gcgcgcttcg atggcaatct ctgcgttttc
    55261 aggttgatac ggtgggatca ctaccgcctg atgcgcctgg cgttgcgctt gcggtaacag
    55321 attaataaat gcttcttcca gcgtggcgct ttgcgtttgc tgccgcagct cttcggcgct
    55381 gccggttgcc agcacttctc cggcattcat cgctaccagc cagtcgaagc gttcggcctc
    55441 ttccatatag gcggtggcga ccagcacgct catattgctc tgccgctggc gaatactgtc
    55501 gatcagatcc cagaactggg cgcgggagag cgggtcaacc cccgttgttg gctcatcaag
    55561 gatcaacagt tccgggtcgt ggattaacgc gcagcacagc ccaagttttt gcttcatccc
    55621 gccggagagt ttacctgccg gacgatcgcg aaacggtgct aacccggtgc tggtcagcag
    55681 ctcattaatt cgcacttccc gctccgcttt atcgtgaccg aacaggcgag cgaaaaaatc
    55741 gacattttca tacaccgaca aggtgtggta gaggtttttg cccagcccct gcggcatcca
    55801 ggcgatgcgc gggcagacgt ctcggcgatg cttcgggtcg tgcatatcgc cacccagcac
    55861 caccacattg ccctgctcaa tgacgcgggc accggaaatc agtgacaata aactcgactt
    55921 cccgacgcca tccggaccaa tcagcccgac catgcaacgg gccggaatat cgagagtgat
    55981 attgttcagc gcaacggttt ttccataatg ctggctcacg cccgccagtt gcgcgacagg
    56041 cgggacagga accagttcca gatgcgtcat tgcggcaacc tcaccacgag gtcgtcaggc
    56101 cacggaagtt cttcattcac ccgcacccac gctacgcccg gtaaaccggt tttgacatat
    56161 tccagatgct gctggagtaa ttccggtggg atacgcgctt tgacgcggaa catcagtttc
    56221 agccgttcat cgctggtttc gacggttttt ggcgtgaact gggcgacact ggcgacaaaa
    56281 ctgatggttg caggaatacg cagatctggc gcggcatcga ggatcagccg ggcttcaccg
    56341 cccagtttca gcgtgcccgc ctgttcggtt ggcaggaaga aggtcatata gacgtcgctg
    56401 agatcgacca tattcagcac ccgaccgcct gccgccagca cttcccctgg ctcggcaacc
    56461 cgatactgca cgcgtccgtc acgcggggct ttcagttcac tgtcatcgat atctgcggca
    56521 atgcgccgtt cagtggcttg cgctgcttcg acccgagttt gcgcctgaat gatattggtg
    56581 cgtgctgctt ctatagccgc tttagaagcc gaaacctggg ctttcgctga ttccagcgca
    56641 gctcgggcgc tctcagcggc ggcgcggtcg tcatccagct gttgcgcaga aatcgcccct
    56701 cgttgggcga gcgagcggga acgcgtatga cgtttggcaa cagagtccag ttcggcctgg
    56761 cgttgattga ccagcgactg tgcggcacga gtttcacttt gtcgttgctc cagcaaagcc
    56821 tgcgcggcgg caacggcgct ttgtgcctct ttgatttgcg cgatggcttc cagtcgctgt
    56881 tcctgcaaca cgcgagtatc catcttcgcc agcacttcgc cttcgcgaac aaactggcct
    56941 tctttcacca gaatggtgtc gatacgcccg gcagttttgc tggcgatatc cacttccgtc
    57001 gcttcaatgc gcccattgct gacagcaaag ccttccggca cacccgccgg gcgcaacagc
    57061 caccacgcca cgacggcagc caccgccagt gccccgacaa cccaccacgc cagatggcgc
    57121 ttactcttat ccataatcga cccgccataa tccctgtaag caaacgacgc gtgttacaac
    57181 tgccgccgaa cgacgttcag cggattcttc catccgggat aagaaggaaa cgacaaatac
    57241 gaccagcgcg cagagcattg agcgccaacg gggaaggctt agcgaaaacg ggtagtaatg
    57301 ttgtcatcat cagcattcct ggccgtaaat gaaacacgca tactcataag tgtgatctat
    57361 aaaactggct gacaaaacgg tatccatcct gttggatcgc gtttcacgcc tgttcagttg
    57421 caggaacaga gcattccttt ttacgtttta tcaacagtct caaccagata gtaacattaa
    57481 tattcatgtt gtctatggtt cagagctgta acattgtgcg ccttctaatc aaaggcataa
    57541 gtccatttct gtttttttca tgctggtaag acttccaggg agtggttatc tctggccctg
    57601 caccaaggtt aatttggcaa ttatgcatgc tgaacattct tccagcatgt ttactgtcac
    57661 gggcgttaag acctaaaact gtccccgttt cacctatgcg ttctctccca ccatttccgt
    57721 tatgtgcaac aacttgattt atacctaaca tttgtgaaca tgctaatagt gcttcaagtt
    57781 taggtctgta atctgtcgaa acagaaaaat aacgattaaa ggatcgacaa gagatagccc
    57841 ggctagtttt aaactttttg ccattaatta tagcatgatg ctttttatta ataatttccg
    57901 ctaattcgac cggatcgaga gcattggtca tttcatcaat agagacagtt atcggtccta
    57961 taatgtaacg ttttcgctga tcatcaaaag ttatcccatg atgatttgcc attattttgt
    58021 atttggggtc atagaaacat acaggataat cttttatccc gtcgtaggta tcctcatcaa
    58081 gtggtttcat gcgcgtgaag ttttgcatat agttaccatt gcaattcgtt tcatgatttc
    58141 cagctaatac atgaacattt tcattgatac tcattatgcg ttttaacaaa tcgatgataa
    58201 attgaacgcc acagatatta ctaaatcgat cgccggtttg gtcgccaaga aacgtacatg
    58261 gcgtgactgc gtctttatta ataaccaaat aaggctgaag actttctaaa acattatctc
    58321 ttctgttcag tatactgatt attgtattaa cattagtgtc aagttgctca ttaaagttct
    58381 cccttagcaa tacgttgaga acttcatctt ctatattaag caactggcta aggaaatttt
    58441 ctccttcagc catgattccc aaatgtcctg ttgcaaaaag cgcgtacaaa actgcaccga
    58501 ctgagctatc ggtatcgcca aaataggtcg gcctggtact tgtgggatca acaggtgaac
    58561 aattggagaa ataggttctg tcagcgaact ggggttttat aattgtttcg aatttaatat
    58621 caaatacatt gtattgcgat atttcccagt tccagatggg tttgtacgac tcagtaactg
    58681 caacatcatt agcattatca tttactgtca tattattaag taaataaaca atgctgcacc
    58741 aattaatatc ggtattatca agagggcaac cgtgattttt actattaatt gtcacaataa
    58801 actgataaca gttattccta cgtagaatat gagcgcgcat gcccgatagc atactatatt
    58861 tttctgaatg attcgtttgt atggtaaatg aaattttgtc atcttgaata gattgttgga
    58921 aacgttctat ttgccaatca tcaatttttt ccacaacttc accgagcatt gtcctggcaa
    58981 tacttgatat atcatcacct ataccatttg caatataccc aaacagttct ttagtgaagt
    59041 tggtaacatg ctcttgcacc atatcatttg ttgcgattgt cgttgtactg ccattagtgc
    59101 ctgccacagt tccaattttc atcttttacc tcatattatt caccgcgagg aatacatagt
    59161 taacatttta tcttatgaca tacacctcat attcgatggg aaaataatta tattttgcac
    59221 aaatcattca gccttaaagc ataatatgaa atttcttatc tcatagcaga gaaagtccct
    59281 aaagattgta gaggcgtcat cagtacatac ctctacaatc gattaagaaa acctatgaaa
    59341 tactggcaaa attaaaaaga gataccacag cactcatgaa gttttattga ggttgaaaac
    59401 aactaagccc tgggacacct tttttatcac cacgttcgtt aggtgctatc ttccaagaat
    59461 ttagccccat ggtataatta tcaatttttt gatgctggtc tacccctaat ccataatggc
    59521 caa
//
LOCUS       DADMEQ010000012        58004 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.012, whole genome shotgun sequence.
ACCESSION   DADMEQ010000012 DADMEQ010000000
VERSION     DADMEQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58004
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.012"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(24..278)
                     /locus_tag="JNZ05_000753"
     CDS             complement(24..278)
                     /locus_tag="JNZ05_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAY8564091.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="JNZ05_000754"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="JNZ05_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HAY8564092.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="JNZ05_000755"
     CDS             1228..2694
                     /locus_tag="JNZ05_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY8564093.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /locus_tag="JNZ05_000756"
     CDS             2812..3798
                     /locus_tag="JNZ05_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAY8564094.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQLL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="JNZ05_000757"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="JNZ05_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HAY8564095.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="JNZ05_000758"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="JNZ05_000758"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HAY8564096.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAMG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="JNZ05_000759"
     CDS             5709..7265
                     /locus_tag="JNZ05_000759"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAY8564097.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVARHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHLVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="JNZ05_000760"
     CDS             7347..9161
                     /locus_tag="JNZ05_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY8564098.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWHFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="JNZ05_000761"
     CDS             9162..10256
                     /locus_tag="JNZ05_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HAY8564099.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="JNZ05_000762"
     CDS             10256..11281
                     /locus_tag="JNZ05_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8564100.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="JNZ05_000763"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="JNZ05_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HAY8564101.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="JNZ05_000764"
     CDS             complement(12876..13220)
                     /locus_tag="JNZ05_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564102.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="JNZ05_000765"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="JNZ05_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HAY8564103.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAVFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="JNZ05_000766"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="JNZ05_000766"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HAY8564104.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="JNZ05_000767"
     CDS             15616..17376
                     /locus_tag="JNZ05_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAY8564105.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDPPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="JNZ05_000768"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="JNZ05_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HAY8564106.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEINVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="JNZ05_000769"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="JNZ05_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HAY8564107.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="JNZ05_000770"
     CDS             19113..19340
                     /locus_tag="JNZ05_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HAY8564108.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="JNZ05_000771"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="JNZ05_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HAY8564109.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDTVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="JNZ05_000772"
     tRNA            21195..21271
                     /locus_tag="JNZ05_000772"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="JNZ05_000773"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="JNZ05_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HAY8564110.1"
                     /translation="MHQSCSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="JNZ05_000774"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="JNZ05_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HAY8564111.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGNKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /locus_tag="JNZ05_000775"
     CDS             complement(25143..26195)
                     /locus_tag="JNZ05_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HAY8564112.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDTNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="JNZ05_000776"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="JNZ05_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HAY8564113.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /locus_tag="JNZ05_000777"
     CDS             complement(26746..28689)
                     /locus_tag="JNZ05_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY8564114.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="JNZ05_000778"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="JNZ05_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HAY8564115.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="JNZ05_000779"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="JNZ05_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HAY8564116.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="JNZ05_000780"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="JNZ05_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HAY8564117.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="JNZ05_000781"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="JNZ05_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HAY8564118.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="JNZ05_000782"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="JNZ05_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HAY8564119.1"
                     /translation="MLEAREVLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="JNZ05_000783"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="JNZ05_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HAY8564120.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="JNZ05_000784"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="JNZ05_000784"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HAY8564121.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="JNZ05_000785"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="JNZ05_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HAY8564122.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="JNZ05_000786"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="JNZ05_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HAY8564123.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="JNZ05_000787"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="JNZ05_000787"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HAY8564124.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="JNZ05_000788"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="JNZ05_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HAY8564125.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="JNZ05_000789"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="JNZ05_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HAY8564126.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37391..37555
                     /locus_tag="JNZ05_000790"
     CDS             37391..37555
                     /locus_tag="JNZ05_000790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564127.1"
                     /translation="MHTPIGVKPAAGSKEWREAWQKRAFAHISNVYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="JNZ05_000791"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="JNZ05_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HAY8564128.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38328..39359)
                     /locus_tag="JNZ05_000792"
     CDS             complement(38328..39359)
                     /locus_tag="JNZ05_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HAY8564129.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRTKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFELVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39593..39952)
                     /locus_tag="JNZ05_000793"
                     /pseudo
     CDS             complement(39593..39952)
                     /locus_tag="JNZ05_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40170..41813)
                     /gene="yojI"
                     /locus_tag="JNZ05_000794"
     CDS             complement(40170..41813)
                     /gene="yojI"
                     /locus_tag="JNZ05_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HAY8564130.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPHAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVPLLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASCDAVARTA"
     gene            complement(41889..42483)
                     /gene="alkB"
                     /locus_tag="JNZ05_000795"
                     /pseudo
     CDS             complement(41889..42483)
                     /gene="alkB"
                     /locus_tag="JNZ05_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
     gene            complement(42483..43547)
                     /gene="ada"
                     /locus_tag="JNZ05_000796"
     CDS             complement(42483..43547)
                     /gene="ada"
                     /locus_tag="JNZ05_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HAY8564131.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43621..44676)
                     /gene="apbE"
                     /locus_tag="JNZ05_000797"
     CDS             complement(43621..44676)
                     /gene="apbE"
                     /locus_tag="JNZ05_000797"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HAY8564132.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44788..45909)
                     /gene="ompC"
                     /locus_tag="JNZ05_000798"
     CDS             complement(44788..45909)
                     /gene="ompC"
                     /locus_tag="JNZ05_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY8564133.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46647..49319
                     /gene="rcsD"
                     /locus_tag="JNZ05_000799"
     CDS             46647..49319
                     /gene="rcsD"
                     /locus_tag="JNZ05_000799"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HAY8564134.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPRIEKYISDIDSYVKSLL"
     gene            49336..49986
                     /gene="rcsB"
                     /locus_tag="JNZ05_000800"
     CDS             49336..49986
                     /gene="rcsB"
                     /locus_tag="JNZ05_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HAY8564135.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGLLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50186..53035)
                     /gene="rcsC"
                     /locus_tag="JNZ05_000801"
     CDS             complement(50186..53035)
                     /gene="rcsC"
                     /locus_tag="JNZ05_000801"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HAY8564136.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53310..54086)
                     /locus_tag="JNZ05_000802"
     CDS             complement(53310..54086)
                     /locus_tag="JNZ05_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HAY8564137.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQISGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54091..55740)
                     /locus_tag="JNZ05_000803"
     CDS             complement(54091..55740)
                     /locus_tag="JNZ05_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HAY8564138.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLAFLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVSSMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPGSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55741..58004)
                     /locus_tag="JNZ05_000804"
                     /pseudo
     CDS             complement(55741..58004)
                     /locus_tag="JNZ05_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
BASE COUNT        14169 a        15655 c        14264 g        13916 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccatc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcggccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccatcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gctcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttgtg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcaatg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gccaggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact tggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcgcccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggca tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgcct atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcgc tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctag gtatcgatac ctggtggtat gacgttaata
     9121 aagcggcaaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcg tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagt acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaaca
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cagcgcactg atgaccatcc
    13861 acgctgccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgaccccccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgct ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctgaccatcg ttgttgacgg
    17701 taaagaaatc aacgttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcagatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccggttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgcccg cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct ggtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagaca ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ttggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccacccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ctttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggtc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga acaagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata atgaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgacggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcaacaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 gtatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gtcaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaggtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcca gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc aggatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgcgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggt
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtacctctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc aaggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgttg gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac ggttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cgggcggata ccgttactgg ctttgcgcca gcgaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgca gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat gtttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgca gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatccg gcataccatg ccggatgtgg cacatcatta caacgcaata
    38341 tccgccactt ctttttgaac gggttgcggt ttcaactgcg gtttcggcgt actatctgct
    38401 gcttgcggtt tgtcgtattt cagccccagc acttcgctgg tgtactgcaa ctcgcgttct
    38461 gttgccgcta catcaccgtt cagcttgcgt ccataagacg gaacgatcgc tttcaacgta
    38521 gcctgccatt gcgggctgga aacgcgatcg ccaaatactt tttccagcag attcaacata
    38581 atcggcgcgg cggttgacgc ccctggcgat gcccccagca gcgccgcaat ggtcccttgc
    38641 tggtcactga ccacttcggt acccagacgc agtacgccac ctttctcggc atcacgcttg
    38701 ataatctgca cacgctgccc cgcttgccac aaacgccagt cctctttttt cgcttgcgga
    38761 tagtactctt tcaacgcttc aaaacgatcc tcttcactca acatcacctg actcaccaga
    38821 tatttcacca gctcgaaatt atccagcccg acgtgcatca tcggcatcac gttagaggtg
    38881 gtggtggaac tcatcagatc ccacaatgaa ccgtttttga ggaatttggt tgagaaggtg
    38941 gcaaatggcc caaacagcac tacgcgttta ccgtccagaa cacgggtatc gatatgcgga
    39001 accgacatcg gtggtgcgcc aacggatgct ttaccgtaaa ctttcgccag atggcgatta
    39061 accacgtctg gattttccga aacaaggaac tgtccgccca ccgggaaacc ggcgtagtct
    39121 ttcgcttccg gaatccccga ttcctgtaac agcttcagcg ccgcaccgcc cgcgccgata
    39181 aagacaaatt tggtgcggat gttctgtgca gtgccgtttt tcagatcggc aacggtaacg
    39241 gtccaggtgt tatcgtcatt acgctttagg gcgcggactt cgctgctgag ttgcagcgag
    39301 aagttagatt tcttctgtaa ggaagcaatt aactggcggg tgatctcacc atagttcaca
    39361 tcagtgccaa tttctgttgc ggatcgcgcc cttccatcac taacggtgcc cactctttga
    39421 tctgcgcgtg atcttcagag taacgcatac cgcgaaacag cgagctttgt tgcaacgcgg
    39481 cgtaacgggc gcgcaggaaa ttaacgttat cctcgcccca gacaaagctc atatgcggaa
    39541 cggtattgat aaatgaacgc ggagtacgca gcacgccgcg ctcgacctgg tgcgcccaga
    39601 actggcggga aatctgaaat gcttcgttaa tggcgactgc tttttcaata ctgatgctgc
    39661 catcggcgtt ttgcggggtg tagttcagtt ccatcagtgc agaatgcccg gttccggcgt
    39721 tgttccagcc gttcgaactc tcctgcgcgc ccccctccag gcgctccacc atggtcatcg
    39781 accattcagg ctccagctcg cgtaaatagg tccccaacgt ggcgctcata atgccgccgc
    39841 caatcaacag tacatcagtt tcctgctcct cggacgcttt cgcttttgcc gccatcgaaa
    39901 cggcattaag ccccacggcc atcgagaaga gcatggcagt cacttttttc atcttgttaa
    39961 tgccttactt ttagtcgctt tattgcaggt gagatttgcg cggcatcaac ggtaacactt
    40021 aagtaacaac atttaaataa tgtttataaa ttatattcta attttagaaa cattataatt
    40081 tcgttgatta attatagggt tattagaaat gaagggattt ttagcatatt gacctgacgg
    40141 cagcagtccg ccaggtcagc gtcgtaatat tatgccgtcc gggcaacggc atcacacgaa
    40201 gcggcatcgc gctcttcgcc cgtcagctcg ctaagttgcc cattgcgcat ttccagcagg
    40261 cggtcggcgt ggataaagta atgatcatca tgactgatag cgaaaatagt tttacccatc
    40321 tcctgcatca gcggcagcaa cacctgataa aactcacgac ggaagtgtgg atcctgatcc
    40381 gccgcccatt catccagcag gataatatcg cgttcttctg ccagcgccag caacagcggc
    40441 acgcgttttt tctgcccttt tgataacttc aggttaacaa tacgcccgtt gcttaactca
    40501 agcttatgag ccattttcag ctgcgccagc cacttctcaa ccagttgcgg gttagcgggt
    40561 tgcccctccg gccccagcag ttgatcaaac agccagacat cggtaaacac tgccgaaaac
    40621 agtttgcgat aatcttccgg ttgttcgccg ctgacaggtt tgccatccag caagatttcg
    40681 ccgctttgtg gctgatacaa gcccgtcaac aacatcgcca gcgtcgattt tccgctaccg
    40741 ttgccgccaa tcagaaacag cagctcgcca cgtttgatgg tgagattaat cggaccaacg
    40801 gaaaacgcgt tatcctgata agcaaacgtc acgttacgca gctccagcgt ttgccagttg
    40861 ggaaacgcat gcgggcgcgg aaactcagct ttgaaaggcg cgagcgcgaa tttgttcagc
    40921 ttgttaaacg ccacctgcgc cgtcagcagc gtcggcaatg cgccaaccgc cgaaagcagc
    40981 ggcgtacgca ggaataacag cgtcaacgaa taggtcgcag caacgttggt atcagcccaa
    41041 ccgagactgt tcgccatcca gaacaccagg ccgatagcgc ccagcatcat gatgtttgac
    41101 cagttcacgg cactaagatg gaaggtgtct gcgcgaataa tatgatggcg atactcttgc
    41161 gcatcgggga tgtagaggtt gttaaacaca tactcggcgc gttcccggtt cagagtcagc
    41221 tctttgcgcc cttcaagtac agtttgaaaa tccgtgtaca gcttgtcttc ggtttcacgt
    41281 agggtcgcca tatgtttgta cacccgcgcc accagcacaa aaccgcccca gatggtgatc
    41341 gccatccaga tagccgttac cagcaacatt ttgcccgaca gcatccacag atacgccgat
    41401 gaaccgatag tgagaatgat cccctgcccc agttccggca gacgcacaaa agcaatggtg
    41461 atattgcgca catcgctggt taatcccgcc agcaacgagg cgctaccgag ttgttcaatg
    41521 cgctcgacgt gggtatccag aatccgcttg ataaattcac tacgcagtcg gtaaacgaag
    41581 tgatgcccca aagtggtgag cgccagttgc gatccgagag tgactgccat caacagcagc
    41641 aataatccca gaaactccgg taacaccagc agactggtat ccgccgtttc gataaggcgc
    41701 tgattgataa aagcaattaa gccaatgcct aatgccgcac tggcgaggct tagcgccatc
    41761 acactgataa atggccagcg atactgccgc cagacaagta caagaagttc catgcagaaa
    41821 acccggacaa tgaattacag cccgcagttt aaacatcttc gcgcgcacag caataataat
    41881 tcttattttt attctttttt acctgcctga cggaatgtca ggttgtagcg gcagtcggtg
    41941 gtgagtggat gaaaccccgc tttcaacggt tgaataccgt gataaaacag ccgcgattca
    42001 ccgccccagg ccaccaaatt gaaaaatcgc gggtaagccc agagaaacag aaacaattgg
    42061 cgcgcgcaga tccggttcgt ctttatcctg atgcagcgac agtttcgcgc caggagcgta
    42121 gcggttgata agacaagcat ctggctggaa atctggatag cccgccgccg tagccgcacg
    42181 ttgacataaa tcatgaaagc tttgtggcat ggcgggccac ggtttatttg tttgcggatc
    42241 aatgggcgaa tagagataac cttgccgatg ggtcgtccag cccagatgcc cacagttggt
    42301 catcgccacc gacatggtat atccgcctgg agcgaccatc tggcgaaacg gcgactggct
    42361 ggcaacgtca ttaatatcgc ggattagttg ctccgcagca ttaaaagcaa aacgccgtaa
    42421 aattaccgca ccagccgcca gtggctcttg ccacggttca gcatcggcaa acagatccaa
    42481 cattacctct cctcattttc agcttcgcgg cgcagcagtt gcgctttacg cgacacgccc
    42541 cagcggtaac cggaaagtgt gccatcacca cggaccactc gatgacaggg tattacgata
    42601 gccagcttgt tggcggcaca ggcgctggca acggcccgta ccgctttcgg tttgccgatg
    42661 gcgttagcca gttgctgata actgacggtt tcaccacaag gtatcgtgcg cagtgcctgc
    42721 cagacttgtt gctgaaaagc agtgccgcga atatccagcg gtaacgtcag cggcgtatcg
    42781 cgttgattga ggctggcgat cacttcacgc acatgttgct gaaacgtcag atcggcaggc
    42841 gcgttgtcgg cagcgggaaa catctgctgc aactcgctga ttaatgtcgc gtcatcatcg
    42901 cccagcaata tcgcgcaaat cccccgctcg ctttctgcca ccaggcaacg acccagctca
    42961 caatcagcca gcgcgtaacg caccgccaga ttttcgccac cgtgacggaa ttgtttagcc
    43021 gtcatgccca gcgtttcatc ggctttgcgg tagtaactgc tgctgtcggg gaatccggcg
    43081 ttaagaatag acgtcgtcac gctctcccct ttcgccagcg attcgcgcaa acggcgagcg
    43141 cgccaggcct gttgccaggc tttaggcgtc attccggtag tagctttaaa caaccgatgt
    43201 agatgaaatg gactcatcgc cacctggtcg gctaaggctt ccagcgttac aggcgtttcc
    43261 tgttccagca gtcgacacgc gtgggtgatt ttatccaacc gatgttgccg gggattggct
    43321 ttgtctggct gacaacgttt gcaggggcga aatccggcgg cgagtgcctc gctggcattt
    43381 gcgtagaagg agacattttc ccgcaaggca tgtctggcgc ggcaagacgg acggcaaaag
    43441 atacccgtgg tgcgcacggc gaaaacgaat tcgccgtcgg cattcgggtc gcgggctaag
    43501 acagattgcc agcgttgatc gtcagttaag catgtggcgt ttttcataat cagctccctg
    43561 gttaaggata gcctttagtc tgcccggtca ccatcacgca aaaaccaaca atcttgcgct
    43621 ttaatttttt tcgctgacaa ggaagctttt aaactgtggt gacatccagg ttttaaagct
    43681 atcgccttct ttggtgatca tataaaccgc cagcccttcc cggcgaacaa cctctttggc
    43741 tttctccggc ccgagtacca tcaagccagt atcccaggca tcggcttcca gcgccgtcgg
    43801 agcaatcacc gtcacggata ccagattgtg ttcgatggga cgcccggttt gcggatcgat
    43861 aacatgggaa agacgtttgc cgtccagttc gtaatagtta cggtagctgc cagaggtgct
    43921 aatcccatga ccgttgatat ccaccacagc ctgaaccgcg ttttctttat cggttggttt
    43981 ttgaatcgct acccgccacg gctggccttc accgttcata ccacggctgt tcagcgcgcc
    44041 gcccaccgac accagatagc gggaaatccc ttcctgctcc atcaagcgtg ccaggtgatc
    44101 cgccgcataa ccttcaccga cggtggagag atcgacatat aaatccggca ggtctttttg
    44161 cagatattgc tgatgcgact gattgatgac cgtcaggtgc tgtaagccgg ttttggcttt
    44221 catcgcatcg atctgttcct ggctcggaat ttgaaccggc tgttgttccg ggccaaagcc
    44281 ccacagattc accagcggcc cgacggtaat atccatcgca ccatcggtcc ttgccccaat
    44341 gcgcagcgag gtggtgacga tatcggccat cgcttcactt accggccacg gcgacaaact
    44401 ttgcgagtcg ttaaagcgca tcagcgcgga atctttttta taggtcgaaa gcagctgatc
    44461 gtcggcgtcc agctgggtct gaatcttttc tttaagttcg gcactgcgtt tggcatcaat
    44521 gcccgggatg ctggcacgcc agaaggtacc catggttttg ccttcaagaa cggtaacttc
    44581 agtagcgtgg gttttggcgg gttgtggttt ttgatcgcaa ccaacaaaga agagcgcggc
    44641 aaccagcagt gccacgcggg taaagcttat ttccatacgt gattatcctc atgcgaacgg
    44701 tcgcaagagt acaccaaaaa actgtgtttg tacgctgaaa acaatgaaaa aagggcccgc
    44761 aggccctttg ttcgatatcc atcgagatta gaactggtaa accagaccca gagctacgat
    44821 gttatcagtg ttgatgccag cgtcacgagt gaactggttg tcgtccagca ggttgatttt
    44881 gtagtcaacg taggtggaca tgtttttgtt gaagtagtag gtcgcaccaa catcaacata
    44941 tttcaggata tcttcgtcgt cgtagttacg accattgatg acacccaggt ttttaccttt
    45001 agactgcagg taagctaggg acggacgcag accgaagtcg aactggtact gagcaacagc
    45061 ttcgaagttc tgtgctttgt tcgcccaacc caagttgcct acgcgagttg cgttgtaggt
    45121 ctgggtgtac tgagcagcta ggtagatgtt gttcgcgtcg tatttcagac caccggtgta
    45181 ggtttcagcg cggtcgccat taccgatgta acgctcatca taacggttca gaccgaagtt
    45241 ctggtcatca gtacgtttgg agctggaaac tgcagcaccg ataccgaagc cttcgtaatc
    45301 ataagtaata gaaccgccaa cgccgtcacc gttctgacgc agagcttcac gaccgttgtt
    45361 ggtcatgcct tcaccttccg ggctaccgtt tttgccctga tactgaacag cgaagttcag
    45421 accgtcaacc agaccaaaga agtcggtgct acggtaggtt gcgaagccgt taccacgctg
    45481 ctgcatgaag ttgtcagaac cgtaagtgtc accaccgaat tctggcagta cgtcggtcca
    45541 ggaagttacg tcgtaaacta cgccgtagtt acgaccgtag tcgaaagaac ctacatcctg
    45601 gaatttcaga cctgcgaatg ccacacgggt ccaggagttg ttttcgtttt cagcgctgtt
    45661 gccctggatc tgatattccc actggccgta accggtcagc tggtcagtaa cctgagtttc
    45721 acctttgaag ccaagacgca tgtaggtctg gtcgccatct acagacttgt cgtcagagaa
    45781 atagtgcagg ccgtctactt taccgtacag atctaatttg ttgccgtctt tgttgtaaac
    45841 ttcagcagcg tttgctgctc ctgctaccag cagagctggg accaggaggg acagtacttt
    45901 aactttcatg ttattaaccc tctgttatat gcctttattt gcttttttat gccactgcat
    45961 actgattaac cctcattaat cagtcggcaa gtccattctc cccaaaaatg cagaataatc
    46021 caacacgaat atgatactaa aacttttaag atgtttcatt tatcgctata gatgtttcaa
    46081 aatgtaaata caagggaact ttttaagatt attgcggaat ggcgaaataa gcacctaaca
    46141 tcaagcaata ataattcaag gttaaaatca ataacttatt cttaagtatt tgacagcact
    46201 gaatgtcaaa acaaaacctt cactcgcaac tagaataact cccgctatca tcattaactt
    46261 tatttattac cgtcattcat ttctgaatgt ctgtttaccc ctatttcaac cggatgcctc
    46321 gcattcggtt tttttaccct tctttacaca cttttcatta ttctgtgcta ccacagaaaa
    46381 actataacgc ttgttaacta tttcacaaat aattaacatc agcataattt ccagcaatct
    46441 ttgtttattt acaattaatt tcgttgggct ttttgtaggt tatttgtaca gcaaaatggc
    46501 gcttgtacat ctatttcccc ccatacagga tgataaatat cacgggagaa tagagaatca
    46561 tcaatcaggt aagagtctgg aatttcacac tgtacccttt atactgccct atcacttcgc
    46621 gaagttttaa caggtcataa acacgaatgc gtcagaaaga gacaacggcc acgacccgct
    46681 tttcactcct accggggagc attacccgct tctttttact gttgatcatt gtgttactgg
    46741 tgacgatggg ggtaatggta caaagtgccg ttaacgcctg gctgaaagat aaaagttacc
    46801 agattgtcga cattacccac gcaatccaaa agcgcgtcga tacctggcgt tacgtgacca
    46861 ggcagatcta cgacaacatt gccgcgacga cctccccctc ctccggcgaa ggtttacaag
    46921 agacgcgcct gaaacaggat gtctactatc tggagaaacc acgccgcaaa acggaagcgt
    46981 taatctttgg ctctcacgac aactcaacgc ttgagatgac tcagcggatg tccacttatc
    47041 tggatacatt gtggggtgca gaaaatgtac cgtggtcgat gtattacctg aatggtcagg
    47101 ataacagtct ggtgctgatc tcaaccctac cccttaaaga tctcacctcc ggatttaaag
    47161 aatcgaccgt cagtgacatt gttgattcac gtcgtgcaga aatgttgcaa caggccaacg
    47221 ccctcgatga acgcgaaagt ttttctaaca tgcgccgcct ggcctggcag aacggtcatt
    47281 actttacctt gcgtaccacg ttcaaccagc cgggacatct ggcaacggtc gtggcttttg
    47341 atctgccgat taatgatttg atcccaccgg gtatgccgct ggacagtttc cgccttgagc
    47401 cagacgcgac ggcaacggga aacaatgata atgagaaaga agggacggat agcgtcagta
    47461 tccactttaa cagtacgaag attgaaatct cctcggcact caactctacc gatatgcgcc
    47521 tggtctggca ggttccttat ggcaccttat tgctggatac gttgcaaaac attctgctgc
    47581 cactgctgct gaacatcggt ttgctggcgc tggcgttatt tggctatacc acattccgcc
    47641 atttctccag ccgcagtaca gaaagcgtcc ccagcacggc ggtcaataac gaattgcgca
    47701 ttttacgggc aattaatgaa gagatagtct cactgttgcc gctcggcctg ctggttcacg
    47761 atcaggaatc gaaccgcact gtcataagta acaaaattgc cgatcatttg ctgccgcatc
    47821 tgaatctgca aaacatcacc accatggcgg aacagcatca ggggattatt caggcgacga
    47881 tcaataacga gctgtatgag atccgcatgt tccgcagcca ggtcgcgccg cgcacacaaa
    47941 ttttcattat tcgcgatcag gatcgcgaag tgctggtaaa caagaaactc aagcaggcgc
    48001 agcgtctgta tgagaaaaac cagcaggggc ggatgacctt tatgaaaaac attggcgatg
    48061 cgctgaaaga acccgcacag tccctggcgg agagtgcggc taaactcaac gccccggaaa
    48121 gcaaacaact ggcgaatcag gcagatgtgc tggtgcggct ggtcgaagaa atacagttag
    48181 cgaacatgct tgcggatgat agctggaaaa gtgagacggt gctgttctcc gtgcaggatt
    48241 taattgatga agttgtgcct tcagtgttgc ctgccatcaa gcgtaaaggt ctgcaactgc
    48301 tgattaacaa tcatctgaaa gcacacgata tgcgccgcgg cgatcgcgat gccttacgac
    48361 gtattttgct gctactgatg caatatgccg tgacctcaac gcaattggga aaaatcaccc
    48421 ttgaggttga tcaggatgag tcctctgaag accgcctgac attccgcatt ctggacacgg
    48481 gagaaggcgt aagtattcat gaaatggata atttgcactt cccgtttatc aaccagaccc
    48541 aaaacgatcg ttatggaaag gcggacccgc tggcattctg gctgagcgat caactggcac
    48601 gtaaactggg cggtcattta aacatcaaaa cgcgggatgg gcttggtaca cgctactctg
    48661 tgcatatcaa aatgctcgcc gctgacccgg aagttgaaga ggaagaagag cgtttactgg
    48721 atgatgtctg cgtaatggtg gatgttactt cggcagaaat tcggaatatt gtcactcgcc
    48781 tgttagaaaa ttggggtgca acctgtatca cacccgatga aagattaatt agtcaagatt
    48841 atgatatctt tttaacggat aatccgtcta atcttactgc ctctggcttg cttttaagcg
    48901 atgatgagtc tggcgtacga gaaattgggc ctggtcaatt gtgcgtcaac ttcaatatga
    48961 gcaacgctat gcaggaagcg gtcttacaat taattgaagt gcaactggcg caggaagagg
    49021 tgacagaatc gcctctgggc ggagatgaaa atgcgcaact ccatgccagc ggctattatg
    49081 cgctctttgt agacacagta ccggatgatg ttaagaggct gtatactgaa gcagcaacca
    49141 gtgactttgc tgcgttagcc caaacggctc atcgtcttaa aggcgtattt gccatgctaa
    49201 atctggtacc cggcaagcag ttatgtgaaa cgctggaaca tctgattcgt gagaaggatg
    49261 ttccaagaat agaaaaatac atcagcgaca ttgacagtta tgtcaagagc ttgctgtagc
    49321 aaggtagcct attacatgaa caatatgaac gtaattattg ccgatgacca tccgatagtc
    49381 ttgttcggta ttcgcaaatc acttgagcaa attgagtggg tgaatgttgt cggcgaattt
    49441 gaagactcta cagcactgat caacaacctg ccgaaactgg atgcgcatgt gttgattacc
    49501 gatctctcca tgcctggcga taagtacggc gatggcatta ccttaatcaa gtacatcaag
    49561 cgccatttcc caagcctgtc gatcattgtt ctgactatga acaacaaccc ggcgattctt
    49621 agtgcggtat tggatctgga tatcgaaggg atcgtgctga aacaaggtgc accgaccgat
    49681 ctgccgaaag ctctcgccgc gctgcagaaa gggaagaaat ttaccccgga aagcgtttct
    49741 cgcctgttgg aaaaaatcag tgctggtggt tacggtgaca agcgtctctc gccaaaagag
    49801 agtgaagttc tgcgcctgtt tgcggaaggc ctcctagtga ccgagatcgc taaaaagctg
    49861 aaccgcagta ttaaaaccat cagtagccag aagaaatctg cgatgatgaa gctgggcgtc
    49921 gagaacgata tcgccctgct gaattatctc tcttctgtga ccttaagtcc ggcagataaa
    49981 gactaatcat ctgtaggcca gataagacgc gttagtgtct tatctggcat ttgcaccgat
    50041 tgccggatgc ggcgtaaacg ccttatccgg cctacgattc ccattatttc aacagattac
    50101 attaaagtag gccagataag acgcgtcagt gtcttatctg gcatttgcac tgaatgccgg
    50161 atgcggcgta aacgccttat ccgtcctacg aatcccgcga tttcctgacc ctctcggcat
    50221 ataccgtcag cgtctgtttt atcacatcca gcgttaccgg cttcgagagg cagctgtcca
    50281 taccggactc cagacaccgc tgcttctctt cagccaacgc attagcagtt actccgatta
    50341 ccggcaacgt cagtcccaac tgacgaatgc gttgcgtcaa gcggtaacca tccatatttg
    50401 gcatgttgac gtcgctaagc acgatatcaa tatgattctt actaagtaca ttaagcgcat
    50461 cgacgccatc attcgcggtt ttacattgat agcccaacga tcccaactga tctgccagca
    50521 gacgtcggtt aatcggatga tcatccacga ccagaatcat catatcgtcg ttatcgctga
    50581 ccgctttgtc cgtcgacggc agagcgttag caggatcgtc gctctccatc tcgatcaaat
    50641 aaatacgcgc caacaatgcc ggtagctcat gcggagcagc cacactgtgt acccactccc
    50701 ctggcgcttt ctccagcgga ataccaatat ggcgacgaca gaaggtcact accgctctgc
    50761 cctgccattt tttactcact acctcgtcag tgattaacac atcttcggga gtcggttcct
    50821 gcccttcgta tgttgtaacg acgatgccgc tgcgctgcaa actggtttcc aggaactgac
    50881 agagcgacgc attgcggacc gccagccagc agcgtttacc actcaaccct tccacgcctt
    50941 ttttctgcgg gtactgagcg ccgtacaacg gaatacgcac ggtaaactgg ctgcccattc
    51001 ccggttctga atctaccgag atatcgccgt ccatcatgct gatcagtttt tcacaaatcg
    51061 ccagacccag accggtcccc tggaagtttc gctgtacgcc cgttccaacc tggaagaagg
    51121 gatcaaacaa gcgcaccact tctttcgccg gaatccccac gccggtatcg cgaacacgga
    51181 tagagagata atcgccatca gcgcgaacat gcaaaactat acagccggta tcggtgaatt
    51241 ttatggcgtt actcaacagg ttggagatga cctgctgtaa acgcatcggg tcgccattta
    51301 aggccactgg cacatccggt tcaataaagc agtacaagcc taactgcttg cgtaccacca
    51361 gcggtaaata gttggcggtg atgtggttca tcacttcacg cggtgaaaac tcacgcggtt
    51421 cgatcttcaa ctgttccgat tcaatcttcg agaaatcgag aatatcgctg ataattttca
    51481 acaacaggct ggaagagttg ttcattgccg tcaccagacg atcgacgcct ttcggtaact
    51541 ctttggtttg caacagatcc aggttaccga taatgccata cagcggcgtt cgcagctcat
    51601 gactgacggt ggcaaggaac atcgattttg actggctcgc ctgttccgct gcttgtgcca
    51661 tctcctgcaa cgactcttcc atcttcacgc gcgaagaaac atccaccagc acacaaatgg
    51721 ccacgttttc attacgatag cgcgaatgga cgaagctgat ttgcagattg gtattgttgc
    51781 tggtcaggac atcaacaaaa ttgacctgct gcccacagat aatttgcgtc agtcgttggc
    51841 ggtcctcatg cgtaagcata ttgagatagg tatgcgccag ttcgttactt aaaatattga
    51901 cgccatcagc ggtacgcaaa atgcagatac ccactggcgc ggaggcgaca atcttgcgat
    51961 tgaactgctc atgttcttcc agtcgcaggg cgtcgctttc cgccggaatg aaaatacgtc
    52021 gctcgtacat ccgcgcgaga gtaaacaatg cagctccggc aagcacattc agcaaaattg
    52081 cgttaaggat caacatgcga atgcgttcca gcaccttatc aaccggcacc gaatacacga
    52141 tgcttagcga tgagggtggc agatttttct tcagcaccag ctcccggaac ccttccgtat
    52201 agccaaacca ggagcgttcc tgcatccagc gaggatcgcc cttaatttta ctttctggtc
    52261 cggtaagcga aatcagggta tgaccatttt catcaagaat ggtaaccccc atcggcaacg
    52321 tacccggtaa gaaaaagttc tccatccgga tggtctgctc gacacccaaa agcgcctgca
    52381 accggttcgc cagataaact ggcgtcaacg cgtaaaaata cccgacgcca gggcgcggac
    52441 cttcgctgat ccagtagagg ttactgccgc tatcatcttg tggtgcattt cgatatttat
    52501 tgatgcgttc atgcaacgct ttcaacgcgg tatcgcgttc cactggcata tcacgcagac
    52561 cgaaattggc catgcagagg ttatcactgc cgattaaaaa tacccggttg agatcgtaag
    52621 ccgcagaaaa attatcgcgc cagtagcgca taaaccacgc caatgactcc agagaacctc
    52681 gccaggtgtt actcattgcg gaacaatcgg agtcggcaaa cagcggttca aacgcaggca
    52741 catccgcctg cgtttctcgt ccacgcgggg aaagcacacc gttttctgcc gataagcgat
    52801 tttcggcgat gtacttaagc tctttcatca catcagaggt gcgttgaata aagcgctgag
    52861 cctgatcgga actcagatta aattcctgac gaatttccga ttctcgctga tgtaacgcat
    52921 taacgatgta aaaaacggat gaaaaagcaa tcaacagcca gagcactaac gccaatgctc
    52981 tgaacatgta gcgcgaggct ttcagggttg tacgaaaaga agcaaggtat ttcaaagggg
    53041 cgaagctccg cctcaggtga ccgatggagt gtggttaagg tagcggtaaa agcgtgttac
    53101 cgcaatgttc tgtcaccatt gcaataatgc gtatcgctta gaaaccattg ccttaaccag
    53161 gaaagccgga gtagatggac agttctcagt acgaaaaata agaaatgacc aatctgccga
    53221 cgcttgttaa tggttatatc ctggcgtcgg cagatcgccc agcagtctta tctgaaaaaa
    53281 gagagagcca ggttgtgtga agggatggat caccagtcaa aacttttcac cagcgtcagc
    53341 tcgccagcat tacgcatcgg tacaataaat gtttcctgtt tctcattgac cgatccttca
    53401 tcggtgatca gcgtcagttg ggcggtcgtc aattccgttt cgctgcgccc accatagtag
    53461 ttgatataca cctgatagcg cccgtgaact ggtgctggca tggcgaaaat ctcgggtccg
    53521 taccccgtcg tgacatccat atccagtgca ccactgtttt tcagcacggt gttaccgtac
    53581 caggcgtgtt cgccatcggg cgtgacaaca tggagatcga gatcggtatt gtccgtatcc
    53641 cacgagagaa ccagccgtaa acgtgcacga atagttcctg tacccggcgt tgagtaaaac
    53701 tgcatttttt gccggctttg cccatccggg ctgatgacct gcacgctgtt gctgccttca
    53761 gtgaaaatat aagggcgtgc aaatgaacca tcggattcaa ttctctgcgg catactggca
    53821 ccgttgacca ccagccggcc ctgctgaact ttacccgccg cagcataatt ctttattttg
    53881 ccgctgatct gcgctgagat attttgatca tctgccatgt tgacggacga cgccgggtaa
    53941 ttgatcgatt gtgaaaaact ggcatcttcg ccctctgccg gatgccagcc agagagcggt
    54001 gcgtcaatct cgacttcctg cacaccttca ctgtgagcga caggcgaaag tgccaccagt
    54061 aacaacggaa gaaaaatctt tcgcatagca ttagtccatt aataattgtc gggtcagcgt
    54121 ttcgatatag ttctcatcaa gcccggtggg ataaccatca aaggccaagt gcagatactc
    54181 atgggttaaa tccagccgat cctgaagcgt aaagaagtta cgaataaaca gccgtttctg
    54241 ctgccgatcg gtatagggga aacctgagac taaacgacag accgcaaaca cgtctggttc
    54301 gttgtacccc gtctcgcctt gtaatatacg ccgccactgc ggcatttttt tcgccagcca
    54361 ggctttcgct ttgggtaata actggcaggt tgagcgtgga gctccccagc ggctaaggct
    54421 gttgtcggga taagcaaacg cgagtatctg gtcgtaacgc tccccctgcc ccgcctgggc
    54481 cgtggcttgc cgccaggaaa gtgtcccttc ggtagcgcga ctgccatgat aatgaaccgg
    54541 atcacctgca tagatgagct cctgcgtcca ggcggtcatc gttcgcgccc ccgtggtcgc
    54601 tggcgaagca gaaacacgct gcgtggcgct gctgccgggg atggtcaggc aatcaccttc
    54661 ccgattggcg ttttgctgta aaaaagtacg tatcgccaca gtcatggctt tcgctgcctc
    54721 aggtggcgta cttttcgcct cgcgatccag aactctggca acgtactctt cacgatcgag
    54781 atgcgactgc aatttcagtt tgcccttttc gctcagtaag gtcgtctcac cgtggctgac
    54841 aaacgtaatg tggttaccat tagtaaacgt cacacgatag cgcccattaa gtacgctggg
    54901 ttttactgcc gtcgtactct tttccgctgt gattttcttc agcggatagc gggcaaacag
    54961 ctccacctct acacattgcc cactggcaac ctgcgtcggc actggcaaaa cccgattaag
    55021 caccgtcgca taacgggtta gtaccgtttt gctggtgcca ctgccagtaa cccacagcgg
    55081 tgtaccatcg gttaaccagc cagcaaaacc accctgacgg atttcctgtt tatcatcggc
    55141 aaaccagctc caggttttca cccgtacccg actaccgagc atggatgaga caccgatttt
    55201 cgcctcgtcc agcaccacat ccagtaagac ttcctgagct ttgttttgcg caggtaacgt
    55261 cgctaatgaa tcaagcagcg atttaaccgt taccgaggtt tctggcttca gcgttgtaag
    55321 aaacgctaac cacgcaggtg cctgacgctg ttgccagtat tgcccccaca catctgcgcc
    55381 aatatgcaac cgctgcggcg caaaatagag cccacaggaa cgtaccagcg cggtatcgcg
    55441 cgttatgctc tccccagcct gacaacaata aacctcttcc ggggagtttc cgcgacactg
    55501 gtagggctgc tccggttgat gggtatcctc cagccaggcg taaacataca gtttccacaa
    55561 actccctaac ggcgtggtga gcgagtcagg taatgctgag actttggtca cgcctgatga
    55621 cgacagctga tacagttgat cgtgctgcgc accgcgcagc accagttgta acggcgtctc
    55681 ctccgcaagc gttggcagag taaccaacgc caatagccag acgattctgc gccagttcac
    55741 ttatttcacc tgcatcccgg tccattcact ccctgccgcg acactttgct gcgcaggtgc
    55801 ataggaacgc acataacgag caggaggcag aacgaattgc cctttttgcg agaagcgcag
    55861 caaatggcgg aaagtgaccg ttcccgtcag ttctttcacc ggcaccatat aggccagttc
    55921 gcccatttca ttacgcgctt tttccagcaa ttgcccctgc tgtttcgcgg cgttgggttt
    55981 attgactgaa atgccccatg ttgtgcgctc aacgtcggct cccggcggga gcggtacttc
    56041 cacctgacca tagcgcagaa ctgcatcctg ctcgcaggta agcgtaattt catcgagata
    56101 cagcgcatcg ctgtcaatct cattgctggt caccggctgc agagtaaagc tcatctcttc
    56161 ttcaccgggg ataagccgat acaactggcg ttcaacggtc accggaatgt tactttgttg
    56221 agccgttttt gccggttcac gccagcggac ctgcacattt tgcggcgata attcgtcacc
    56281 aaaagagaga atgtccggca caccctgacc aacccaacgc cagtcttcgc cccctccagt
    56341 taacttatgt ttagcccatg cgcccgcagg cgcaggcaaa acaactggag gcattgtcgc
    56401 catatatttc gccagccagt tcatggccag cgcgcgctca atagtggatt gctcagcggt
    56461 caaaccatta aaatagcggc cgtctgggta gcgtcaccac cgctattgag cagcacaacc
    56521 gtgtgcgcca gcggctgatt gctgtttgcc gctttatccc acgcatattg ctgaacctgt
    56581 ggcatcaccg tggacttcaa ctgcgctttt ttcagcaacg tcatggttaa gacgtttgcc
    56641 accgcatctg ccagtggaga ttccggtgta tcgaggatca ggctgtcatt gatatcgcgg
    56701 atgtcttcct cagagaaatc ttcatcttta gttccgcgcc gggcgatagc ttcatccccc
    56761 cctttcaaca acgttttgca cggcagattc atctcctgtg cccacgccag caccagcgcc
    56821 cgatgcaata acggcatgtt atccgcctgc tcggcataac tgtcgagcat atgctgccag
    56881 tattccggtt gttgcgttac gccgagcgcc tggctggcct gccaatcggc gtaccatgcc
    56941 catgccgtaa ggaaggcgtc accattgcca tcttcacccc accaggtaaa gcgcgctccg
    57001 ggccccgcca gttgcatcag ccgcagacgg ttatcctgaa tcatctgacg aatgtcgtta
    57061 gcggcggcac tttgatgatc ggcaagcgaa cgccaggcga gactgagcgg gatcagacgg
    57121 ctaccggtgt tgatcacccc accccacggt tcatcgacta acgcatcaag attgttgcgg
    57181 aaaatctcct gcggcgtttc actactttgt agccggatat tactcgcctg ctcgggcaac
    57241 atcagcgcgt tctcgccacc gccgagcatg acatttttct gctgttcaac gggccagctg
    57301 ttgtccacaa aagacagttt tgtgctaatg ctgtcctgca cttgcccatt ttgttgcagt
    57361 tctgcactta acaagccaga ttgctgaata ttctgcgtca gcgaaatata attcgcccct
    57421 ttgtgcagcg tcagcgtctg gcgcatctca gcgcctgcaa atttagtcac cagcgccacc
    57481 ggttcgttat cctgctgact gaagataaac agtcctgccg ccggtttgtc gcccacgcga
    57541 tacaccgttg gcatactcca cttcatgtag agattttttt ccgaacgcag ataagcacgc
    57601 ccctgcccga ccagcccgtc gccgttcatc ccacgcgcgg tgatacgcca gcgggttaac
    57661 gaatcaggca tcaggaacgt gaaatacgct ttgccttgtt tatcggttgt gagtgacggc
    57721 atccatgccg cggtatccac ctcttcacgc cgtggacgtt caagcatttt tactcgccgc
    57781 tcgctgcggt tagtcgcgcc aggcgcaacc ggctcgctgg agagcgcctg gtcatagctg
    57841 ataaacgaca gactggagct ggtacgcaca ttgttacgcc ccagcggata gaaaaatttg
    57901 ccgatattcg gcgcgatttc tggttgcagc gcgtagatca tttcatcgac cacgcctacc
    57961 gttagctgcg cagaaacagg tttacctttc agcgacgagg ttaa
//
LOCUS       DADMEQ010000013        55712 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.013, whole genome shotgun sequence.
ACCESSION   DADMEQ010000013 DADMEQ010000000
VERSION     DADMEQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.013"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            77..865
                     /gene="modE"
                     /locus_tag="JNZ05_000805"
     CDS             77..865
                     /gene="modE"
                     /locus_tag="JNZ05_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HAY8564139.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIAALC"
     gene            933..2405
                     /gene="modF"
                     /locus_tag="JNZ05_000806"
     CDS             933..2405
                     /gene="modF"
                     /locus_tag="JNZ05_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HAY8564140.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            2666..3682
                     /gene="galE"
                     /locus_tag="JNZ05_000807"
     CDS             2666..3682
                     /gene="galE"
                     /locus_tag="JNZ05_000807"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAY8564141.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            3692..4738
                     /gene="galT"
                     /locus_tag="JNZ05_000808"
     CDS             3692..4738
                     /gene="galT"
                     /locus_tag="JNZ05_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HAY8564142.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNETEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            4742..5890
                     /gene="galK"
                     /locus_tag="JNZ05_000809"
     CDS             4742..5890
                     /gene="galK"
                     /locus_tag="JNZ05_000809"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAY8564143.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            5884..6924
                     /gene="galM"
                     /locus_tag="JNZ05_000810"
     CDS             5884..6924
                     /gene="galM"
                     /locus_tag="JNZ05_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HAY8564144.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYTNRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            7127..7879
                     /gene="gpmA"
                     /locus_tag="JNZ05_000811"
     CDS             7127..7879
                     /gene="gpmA"
                     /locus_tag="JNZ05_000811"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAY8564145.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(8046..9098)
                     /gene="aroG"
                     /locus_tag="JNZ05_000812"
     CDS             complement(8046..9098)
                     /gene="aroG"
                     /locus_tag="JNZ05_000812"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HAY8564146.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            9414..9794
                     /locus_tag="JNZ05_000813"
     CDS             9414..9794
                     /locus_tag="JNZ05_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564147.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            9908..10849
                     /gene="zitB"
                     /locus_tag="JNZ05_000814"
     CDS             9908..10849
                     /gene="zitB"
                     /locus_tag="JNZ05_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HAY8564148.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(10846..11565)
                     /locus_tag="JNZ05_000815"
     CDS             complement(10846..11565)
                     /locus_tag="JNZ05_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HAY8564149.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(11603..12646)
                     /gene="nadA"
                     /locus_tag="JNZ05_000816"
     CDS             complement(11603..12646)
                     /gene="nadA"
                     /locus_tag="JNZ05_000816"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAY8564150.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKTRADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAVTLRG"
     gene            complement(12895..12970)
                     /locus_tag="JNZ05_000817"
     tRNA            complement(12895..12970)
                     /locus_tag="JNZ05_000817"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12935..12937),aa:Lys,seq:ttt)
     gene            complement(12974..13049)
                     /locus_tag="JNZ05_000818"
     tRNA            complement(12974..13049)
                     /locus_tag="JNZ05_000818"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13014..13016),aa:Val,seq:tac)
     gene            complement(13101..13176)
                     /locus_tag="JNZ05_000819"
     tRNA            complement(13101..13176)
                     /locus_tag="JNZ05_000819"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13141..13143),aa:Lys,seq:ttt)
     gene            complement(13179..13254)
                     /locus_tag="JNZ05_000820"
     tRNA            complement(13179..13254)
                     /locus_tag="JNZ05_000820"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13219..13221),aa:Val,seq:tac)
     gene            complement(13391..13466)
                     /locus_tag="JNZ05_000821"
     tRNA            complement(13391..13466)
                     /locus_tag="JNZ05_000821"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13431..13433),aa:Lys,seq:ttt)
     gene            complement(13631..14422)
                     /gene="cpoB"
                     /locus_tag="JNZ05_000822"
     CDS             complement(13631..14422)
                     /gene="cpoB"
                     /locus_tag="JNZ05_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HAY8564151.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(14432..14953)
                     /gene="pal"
                     /locus_tag="JNZ05_000823"
     CDS             complement(14432..14953)
                     /gene="pal"
                     /locus_tag="JNZ05_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HAY8564152.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(14988..16280)
                     /gene="tolB"
                     /locus_tag="JNZ05_000824"
     CDS             complement(14988..16280)
                     /gene="tolB"
                     /locus_tag="JNZ05_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HAY8564153.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(16413..17654)
                     /gene="tolA"
                     /locus_tag="JNZ05_000825"
     CDS             complement(16413..17654)
                     /gene="tolA"
                     /locus_tag="JNZ05_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HAY8564154.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(17719..18147)
                     /gene="tolR"
                     /locus_tag="JNZ05_000826"
     CDS             complement(17719..18147)
                     /gene="tolR"
                     /locus_tag="JNZ05_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HAY8564155.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(18151..18843)
                     /gene="tolQ"
                     /locus_tag="JNZ05_000827"
     CDS             complement(18151..18843)
                     /gene="tolQ"
                     /locus_tag="JNZ05_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HAY8564156.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(18840..19244)
                     /gene="ybgC"
                     /locus_tag="JNZ05_000828"
     CDS             complement(18840..19244)
                     /gene="ybgC"
                     /locus_tag="JNZ05_000828"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAY8564157.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQHIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(19394..19687)
                     /gene="ybgE"
                     /locus_tag="JNZ05_000829"
     CDS             complement(19394..19687)
                     /gene="ybgE"
                     /locus_tag="JNZ05_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HAY8564158.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(19687..19800)
                     /gene="cydX"
                     /locus_tag="JNZ05_000830"
     CDS             complement(19687..19800)
                     /gene="cydX"
                     /locus_tag="JNZ05_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HAY8564159.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(19815..20954)
                     /gene="cydB"
                     /locus_tag="JNZ05_000831"
     CDS             complement(19815..20954)
                     /gene="cydB"
                     /locus_tag="JNZ05_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY8564160.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTGGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(20970..22538)
                     /gene="cydA"
                     /locus_tag="JNZ05_000832"
     CDS             complement(20970..22538)
                     /gene="cydA"
                     /locus_tag="JNZ05_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HAY8564161.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILIANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(23387..26038)
                     /gene="mngB"
                     /locus_tag="JNZ05_000833"
     CDS             complement(23387..26038)
                     /gene="mngB"
                     /locus_tag="JNZ05_000833"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HAY8564162.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGFRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLEPLEPLATLAWTLGFEYHHGLLEKI
                     WKEILKNHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADK
                     LVLFNLMPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHY
                     GNYDPFMEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNED
                     GTLQLVDKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQ
                     SRAVIRYEMAVPRNLQQRSARQSTGRVGVELVVTLSHNNRRIDVDINLDNQADDHRLR
                     VLIPTSFNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGR
                     NGMAVFSEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQL
                     RGLLSCRLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYS
                     LLKMPPVGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHI
                     TTEENQGSNLSGPFLPGQSRTFSYRLA"
     gene            complement(26056..28032)
                     /gene="mngA"
                     /locus_tag="JNZ05_000834"
     CDS             complement(26056..28032)
                     /gene="mngA"
                     /locus_tag="JNZ05_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HAY8564163.1"
                     /translation="MVLFYRAHWRDYKNDQVRIMMNLTTLTHRDALCLNARFTSREEA
                     IHALTQRLAALGKISSTEQFLKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVAT
                     LSEPLQWESVDGPEAVDLVVLLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATT
                     PDELLSALDDKGGTQPSASFSNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVY
                     VEKQGANGIEGRLTADQLNSATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEAL
                     IQQSLTLERGDETRTVQQDTQPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLL
                     SQIFGLQDLFNEENSWLWMYRKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGL
                     AANMIGSGFLGAVVGGLIAGYLIRWMKNHLRLSSKFNGFLTFYLYLVLGTLGAGSLML
                     FVVGEPVAWINNSLTAWLNGLSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMA
                     NGVYGPYAIFASVKMVSAFTVTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPM
                     AIEDPLRVIGSFVLGSMVTGAIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWF
                     GAALVGAAISTAILLIWRRHAVKHGNYLTDGVMQ"
     gene            28142..28864
                     /gene="mngR"
                     /locus_tag="JNZ05_000835"
     CDS             28142..28864
                     /gene="mngR"
                     /locus_tag="JNZ05_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HAY8564164.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(28968..29837)
                     /gene="sucD"
                     /locus_tag="JNZ05_000836"
     CDS             complement(28968..29837)
                     /gene="sucD"
                     /locus_tag="JNZ05_000836"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HAY8564165.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(29837..31003)
                     /gene="sucC"
                     /locus_tag="JNZ05_000837"
     CDS             complement(29837..31003)
                     /gene="sucC"
                     /locus_tag="JNZ05_000837"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HAY8564166.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(31097..32314)
                     /gene="odhB"
                     /locus_tag="JNZ05_000838"
     CDS             complement(31097..32314)
                     /gene="odhB"
                     /locus_tag="JNZ05_000838"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HAY8564167.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(32329..35130)
                     /gene="sucA"
                     /locus_tag="JNZ05_000839"
     CDS             complement(32329..35130)
                     /gene="sucA"
                     /locus_tag="JNZ05_000839"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HAY8564168.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(35431..36147)
                     /locus_tag="JNZ05_000840"
     CDS             complement(35431..36147)
                     /locus_tag="JNZ05_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HAY8564169.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(36163..37929)
                     /gene="sdhA"
                     /locus_tag="JNZ05_000841"
     CDS             complement(36163..37929)
                     /gene="sdhA"
                     /locus_tag="JNZ05_000841"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HAY8564170.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(37929..38276)
                     /gene="sdhD"
                     /locus_tag="JNZ05_000842"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(37929..38276)
                     /gene="sdhD"
                     /locus_tag="JNZ05_000842"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HAY8564171.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(38270..38674)
                     /gene="sdhC"
                     /locus_tag="JNZ05_000843"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(38270..38674)
                     /gene="sdhC"
                     /locus_tag="JNZ05_000843"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY8564172.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            39055..39162
                     /locus_tag="JNZ05_000844"
     CDS             39055..39162
                     /locus_tag="JNZ05_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564173.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            39368..40651
                     /gene="gltA"
                     /locus_tag="JNZ05_000845"
     CDS             39368..40651
                     /gene="gltA"
                     /locus_tag="JNZ05_000845"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HAY8564174.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            41042..41607
                     /locus_tag="JNZ05_000846"
                     /pseudo
     CDS             41042..41607
                     /locus_tag="JNZ05_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            41658..44117
                     /locus_tag="JNZ05_000847"
     CDS             41658..44117
                     /locus_tag="JNZ05_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY8564175.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWYSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            44132..44863
                     /locus_tag="JNZ05_000848"
     CDS             44132..44863
                     /locus_tag="JNZ05_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY8564176.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            44860..45921
                     /locus_tag="JNZ05_000849"
     CDS             44860..45921
                     /locus_tag="JNZ05_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8564177.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIHVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            46028..47119
                     /locus_tag="JNZ05_000850"
     CDS             46028..47119
                     /locus_tag="JNZ05_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAY8564178.1"
                     /translation="MLYRYTKEKIMRERRMPVLQWGMLCVLSLLLSIGFLAVHLPAAL
                     LLGPMIAGIIFSMRGITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAI
                     LLVTLLSSAIVGWLLVRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYL
                     RVLFVAGAAVLVTRMMLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLP
                     SGTMLIPMLAGAVLQSGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQ
                     ILLSIFALLAICAGMAWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMA
                     MQTLRLFSILLTGPAIARFISTYAPKRSA"
     gene            complement(47116..47907)
                     /gene="nei"
                     /locus_tag="JNZ05_000851"
     CDS             complement(47116..47907)
                     /gene="nei"
                     /locus_tag="JNZ05_000851"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HAY8564179.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(47943..48677)
                     /gene="pxpA"
                     /locus_tag="JNZ05_000852"
     CDS             complement(47943..48677)
                     /gene="pxpA"
                     /locus_tag="JNZ05_000852"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HAY8564180.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKETQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(48667..49599)
                     /gene="pxpC"
                     /locus_tag="JNZ05_000853"
     CDS             complement(48667..49599)
                     /gene="pxpC"
                     /locus_tag="JNZ05_000853"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HAY8564181.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(49593..50249)
                     /gene="pxpB"
                     /locus_tag="JNZ05_000854"
     CDS             complement(49593..50249)
                     /gene="pxpB"
                     /locus_tag="JNZ05_000854"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HAY8564182.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(50272..51015)
                     /gene="ybgI"
                     /locus_tag="JNZ05_000855"
     CDS             complement(50272..51015)
                     /gene="ybgI"
                     /locus_tag="JNZ05_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HAY8564183.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLENDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            51286..52767
                     /gene="dtpD"
                     /locus_tag="JNZ05_000856"
     CDS             51286..52767
                     /gene="dtpD"
                     /locus_tag="JNZ05_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HAY8564184.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTLF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(52917..54335)
                     /gene="phrB"
                     /locus_tag="JNZ05_000857"
     CDS             complement(52917..54335)
                     /gene="phrB"
                     /locus_tag="JNZ05_000857"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HAY8564185.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(54332..54841)
                     /locus_tag="JNZ05_000858"
     CDS             complement(54332..54841)
                     /locus_tag="JNZ05_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HAY8564186.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            54970..55287
                     /locus_tag="JNZ05_000859"
     CDS             54970..55287
                     /locus_tag="JNZ05_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HAY8564187.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSLHLK
                     GDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELREELYQVKQVVKGMRDGMFSVL
                     NES"
     gene            complement(55342..55662)
                     /locus_tag="JNZ05_000860"
     CDS             complement(55342..55662)
                     /locus_tag="JNZ05_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564188.1"
                     /translation="MPGYRTWIEISLIDELKPSYPFRLEEYAMIGHSPYANECNKDEA
                     KFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELKTRLSTLHVNLEQFINSSEDD
                     EYTL"
BASE COUNT        13634 a        14852 c        13634 g        13592 t
ORIGIN      
        1 gcactcccct tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt
       61 ttgccaggac attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt
      121 attcgccgac ccgcgccgca tttcgctact aaaacacatt gcgctttccg gttccattag
      181 ccagggagcg aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat
      241 gaatcagtta agtgagcata ttctggtcga gcgcgcaaca ggcggtaaag gtggcggagg
      301 cgcagtactg acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat
      361 ccagcaaaaa gcctttgatg tgttaagtga tgatgacgcc ctgccgctga acagcctgct
      421 ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat
      481 caccgcccgc gatcatgatg acgttcaaca gcatgttgat gtcttactgg ctgacggaaa
      541 aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg
      601 caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt
      661 cgcacaaaac gccgacaacc aattaccggg tattattagt catattgagc gcggcgcaga
      721 gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt
      781 aaatgaagcg acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag
      841 cgtgattatc gccgcgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct
      901 gtcagtaatc gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca
      961 cgtttcgtct tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg
     1021 atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc ggcgctggcc cgcgcgctgg
     1081 cgggggaact tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc
     1141 tctccttcga gcaattgcaa aagctcgtca gcgacgaatg gcaacggaat aacaccgata
     1201 tgctcggccc tagcgaagat gacaccggac gcactacggc tgagattatt caggatgaag
     1261 taaaggatgc accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg
     1321 accgacgctt taaatacctt tccactggcg agacgcgaaa aaccctgctg tgtcaggcgc
     1381 tgatgtcgga gcctgacttg ttgattcttg atgagccgtt cgatggcctg gatgttgcct
     1441 cacgtcagca gctggctgag cgactcgcct cgttacatca gtccggtatt actttggtac
     1501 tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca gtttgctggc gtgctggcgg
     1561 attgtacgtt agcggaaact ggcgctaaag aggaactgct ccagcaagca ctcgtcgcgc
     1621 aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag
     1681 cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct gaacaatggc gtggtttctt
     1741 ataatgatcg ccccattctt aataacctta gctggcaggt gaatccaggc gaacactggc
     1801 aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt aagcctgatt actggcgatc
     1861 atccgcaagg ttacagcaac gatttgacgc ttttcggccg acgtcgcggc agcagcgaaa
     1921 ccatctggga catcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc
     1981 gggtcagcac taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt
     2041 atcaggccgt ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca
     2101 ttgataaaca cacggctgac gctccgttcc atagtctttc ctggggacag cagcgtctgg
     2161 cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac
     2221 aggggcttga tccgctcaat cgccaactta tccgccgttt tgttgatgtg ctgattagcg
     2281 aaggtgaaac gcaattgttg tttgtttcgc accacgctga agatgcgcct gcctgtatta
     2341 cccatcgcct tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat
     2401 attgagtcgg tagtgctgac cttgccggag gcggcctaag cgccctctcc ggccaacggt
     2461 tcgacgcatg caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa
     2521 accctctgtt ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg
     2581 attccactaa tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca
     2641 taccataagc ctaatggagc gaattatgag agttctggtt accggtggta gcggttacat
     2701 tggaagtcat acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa
     2761 cctctgtaac agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg gcaaacatcc
     2821 aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg accgagatcc tgcacgatca
     2881 cgctatcgac accgtgatcc acttcgccgg gctgaaagcc gtcgccgaat cggtacaaaa
     2941 accgctggaa tattacgaca acaatgtcaa cggcactctg cgcctgatta gcgccatgcg
     3001 cgccgctaac gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc
     3061 ccaaattcca tacgttgaaa gcttcccgac cggcacaccg caaagccctt atggcaaaag
     3121 caaattgatg gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg actggagcat
     3181 tgccctgctg cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga
     3241 tccgcaaggc attccgaata acctgatgcc atacatcgcc caagttgctg taggccgtcg
     3301 cgactcgctg gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga
     3361 ttacatccac gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa
     3421 caagccaggc gtacacatct acaacctcgg tgctggcgta ggcagcagcg tgctggacgt
     3481 ggttaatgcc ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg
     3541 cgaaggcgac cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg
     3601 gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca
     3661 tccacaggga tatcccgatt aaggaacgac catgacgcaa tttaatcccg ttgatcatcc
     3721 acatcgccgc tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa
     3781 gcgcccctgg cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc
     3841 agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc ccgattacac
     3901 cgggacttac gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc
     3961 agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg
     4021 cttttcaccg gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat
     4081 cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt
     4141 ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg
     4201 ggcaaatagc ttcctgccta acgaaactga gcgcgaagac cgcctgcaaa aagaatattt
     4261 tgccgaacag aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag
     4321 ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc
     4381 gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc
     4441 ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt
     4501 ccagtgctcc ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa
     4561 tcaacactgg cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg
     4621 taaatttatg gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca
     4681 ggcagcagag cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga
     4741 aatgagtctg aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac
     4801 tcacaccatt caggcgcctg gccgcgtgaa tttgattggt gaacacaccg actacaacga
     4861 cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg caccacgcga
     4921 tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct
     4981 caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt
     5041 gaaacatctg caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa
     5101 tgtgccgcag ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt
     5161 attgcagcag ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga
     5221 agcagaaaac cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact
     5281 cggcaagaaa gatcatgcct tgctgattga ctgccgctca ctggggacta aagcagtttc
     5341 catgccgaaa ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg
     5401 cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc
     5461 agctctgcgc gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat
     5521 cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag
     5581 cgcgctggag caaggcgacc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc
     5641 tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa
     5701 agctgtgatt ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat
     5761 cgtcgcgctg atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata
     5821 tgaagcaaaa acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg
     5881 acagtgctga acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact
     5941 ttgcgtaaca acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttcc
     6001 gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagtccg
     6061 gaatgctatc aggatcaggc tgcgtttctg ggggcctcta ttggtcgtta taccaaccgc
     6121 atcgccaata gtcgttatac ctttgacggt gaaaccgtga cgctttcgcc aagccagggc
     6181 gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac
     6241 cagaacgatc gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg
     6301 ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact
     6361 tatcgcgcca cagttgataa accttgcccg gtgaatatga ctaatcacgt ctatttcaat
     6421 cttgacggcg agcagtctga cgtgcgcaat cacaagttgc agattctggc ggacgaatat
     6481 cttccggttg atgaaggcgg cattccgcac gacggcttga aatctgtcgc cggaacgtct
     6541 tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc
     6601 aaagtgaaag gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg
     6661 gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccg
     6721 gctcttcaat tctactccgg caacttcctc ggcggcacac cgtcgcgggg aaccgaacct
     6781 tacgccgact ggcaagggct ggcgttggaa agcgagtttc tgccggacag cccgaaccac
     6841 cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg
     6901 gaatatcagt ttattgctga gtaatgttaa gccctcgtca tgagggcttt atcttcatat
     6961 tgttcaaatc accagcaaac accgacatat ttgcaactca atattcacaa caaccttaca
     7021 ctgcgccact attttcgcta tggttatgcg taagcattgc tgttgcttcg tcacggcaat
     7081 ataatgagaa ttattatcat taaaagatga tttgaggagt aagtatatgg ctgtaactaa
     7141 gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt tcaccggttg
     7201 gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca aaagcagcag gtaagctgct
     7261 gaaagaggaa ggttacagct ttgactttgc ttacacttct gtgctgaaac gcgctatcca
     7321 taccctgtgg aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg agaaatcctg
     7381 gaaactgaac gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg aaactgctga
     7441 aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt tttgcagtga ctccgccgga
     7501 actgactaaa gatgatgagc gttatccggg tcacgatccg cgttacgcga aactgagcga
     7561 gaaagaattg ccgctgacgg aaagcctggc gctgaccatt gaccgcgtga tcccttactg
     7621 gaatgaaacc attctgccgc gtatgaagag cggtgagcgc gtaatcatcg ccgcacacgg
     7681 taactccctg cgtgcgctgg tgaaatatct tgataacatg agcgaagaag agattcttga
     7741 gctgaatatc ccgactggcg tgccgctggt gtatgagttc gacgagaatt tcaaaccgct
     7801 gaaacgctat tatctgggta atgctgacga gatcgcggcg aaagcagcag ccgttgcgaa
     7861 ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg agaaagccga ctgcaagtga
     7921 gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat tgcctgatgc gacgctgcgc
     7981 gtcttatcag gcctgtggtg attcatcgga tacgccactc tgacggcgca tccgacaatt
     8041 aaaccttacc cgcgacgcgc ttttactgca ttcgccagtt gacgtaacag agcatcggta
     8101 tcttcccagc caatgcaggc atcggtgatg ctcttaccgt aggccagcgg ctccccgcta
     8161 tcgaggctct gattgccttc caccagatgg ctttccacca tcacgccaat aatggccttt
     8221 tcgccaccgg caatctgctg gcaaacgtca gcacaaacat ccatctgctt tttgaattgt
     8281 ttggacgaat tagcatggct gaaatcgatc atcacctgag ctggcagacc tgctttgttc
     8341 agcccttctt tcacttcagc aacgtgcttc gcgctgtagt taggctcttt accgccgcgc
     8401 agaatgatat ggcaatcgcc gttaccactg gtattcacaa tcgccgaatg cccccattta
     8461 gttacggaca ggaagcagtg cggcgcaccg gcggcattaa tggcatcgat agccacttta
     8521 atcgtaccgt ctgtgccatt tttaaaacct accggacaag aaagaccaga cgccagttcg
     8581 cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc cccagctcat caggtcagcg
     8641 agatactgag gagtgatcat atcgagaaac tcacccgccg ctggcagacc gctgtcgtta
     8701 atatcaagca gcaatttacg ggctatacgc agaccgtcgt tgatctggaa gctgttatcc
     8761 atatgcggat cgttaatcaa ccctttccag cccaccgtgg tacgcggctt ttcaaaatag
     8821 acgcgcatta cgatttccag ctcatctttc agctcttcac gcagcgccag caagcgagtg
     8881 gcatactctt tagccgcgac aggatcatga attgagcacg ggccaatcac caccaacagg
     8941 cgatcatcat tacctttcag gatcttatgg atcgcttttc gggcatgagc aaccgtattc
     9001 gcggcatttt cagttgcggg gaatttttcc agcaatgcga caggaggaag taactctttg
     9061 atttctttga tgcgtaaatc gtcgttctga taattcatgt ctgttccagt gtcgccatac
     9121 ttatcttagt gaatgcaata cttccaatct atatcttccg tcagaatgtg taaacggggt
     9181 tttacactat gaacggatta atcctagaat tagcaagaaa aacgccagat tgtcgcgaaa
     9241 aacgagatct ttcctacaat ttctaactgt aactcctttg tttgttaatt atttcaagat
     9301 tctctgctgc gtttcataaa ctggctgaaa tcttaaacca atgccttata ttcacctgca
     9361 aatgcactgt tggaagaggt tatccgacat aacgaccata acaggagcat cctatgaaaa
     9421 tgacaaaact ggccacatta tttctgactg ccactctaag ccttgccagc ggtgccgcac
     9481 tggctgccga tagcggagcg caaactaata acggccaggc aaacgccgca gctgatgcgg
     9541 gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa taacgtcgac aataacgggg
     9601 taaataccgg ttctggcggc acaatgctgc attcggatgg ttcttcaatg aacaatgacg
     9661 gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa agatggtcga tgtccggata
     9721 tcaataaaaa ggtacaaacc ggtgatggca tcaataatga tgtcgatacc aaaaccgacg
     9781 gtaccacgca gtaaccctgc aaaatagcca ataatgggag agcggagtct ctccctttta
     9841 atttttcact tctaaaagca gtatgatgtc ataaagttaa gtgagaataa ttaagggatg
     9901 actcgtcatg gcgcactcac actcacacac gtcctcacac ctgccagaag ataataatgc
     9961 tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt atgctggtag aagtcgttgg
    10021 tggttttctt tctggttctc tggcattgct ggccgatgcg ggtcatatgt tgaccgatac
    10081 tgccgccctg cttttcgccc tactcgccgt gcaattttca cgtcgtcctc ccaccattcg
    10141 ccacactttc ggttggctaa gactgaccac gctcgcggcg tttgtgaatg ccatcgccct
    10201 cgtggtaatt accattttga ttgtctggga ggcgatagaa cgcttccgca cgccgcgtcc
    10261 ggtcgaggga ggcatgatga tggcaattgc cgtggctggg ttgctggcaa atatactttc
    10321 tttctggtta cttcatcacg gcagcgaaga gaaaaacctc aacgtacgag cagcggcact
    10381 gcatgtgctc ggggacctgc tgggttcggt tggggctatt attgccgcgt tgattattat
    10441 ctggactggc tggacacctg ctgaccccat tctctcgata ctggtgtcgc ttctggttct
    10501 gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa ttacttgaag gtgcaccggt
    10561 atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg gaaatcccgg aagtccgcaa
    10621 tgtgcaccat gtacatgtat ggatggtagg cgagaagccg gtgatgacgt tgcatgtgca
    10681 ggtgatcccg ccacacgatc acgatgcctt gttggatcag atccaacact acctgatgga
    10741 tcactatcag attgagcacg ccaccattca gatggaatat caaccttgtc atgggccaga
    10801 ctgccacctg aatgaagggg tgtctggcca ttcacatcat caccattaat gggacagcgc
    10861 gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg ctgccgttga gcgcaataaa
    10921 ggtcaggatg atgtactcca gagacatggc gtaaacgccc tgaagtgcaa aaataacgac
    10981 gctaatcacg ttaataatca cccacaacag ccagttttcc acatacttac gcgtcatcag
    11041 aatcattgcc acgatagata acaccatcat gcatgaatcc cagaacggga aagcgtccgg
    11101 ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc atgatcatga ctgccacgcg
    11161 ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc agaccaatcg aaacaacgca
    11221 aaccgccaac cagctgagtg ccttcggcaa tggcaaccag cgaattttca actccgcctc
    11281 gttctgactg gtttgtcgcg accacgcata ccagccgtaa atattcgcgg caaagaaaaa
    11341 cacctgtaat agcaggctgg catacagctg aatctgaaag aaaataatgc caaacaaggt
    11401 aacgttgatc aggccaaaga agtagttgct gatcttctcc agactggcaa ggccaataca
    11461 cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag agatcataac cgcccgcccc
    11521 tattggtata tgtaccagga tattctgcac actaaaaaaa tccatctttc ccccaaagcg
    11581 taacgttacg ccttattatt cgttatccac gtagtgtaac cgcaaaatcc agcatacgat
    11641 tgagcggcac cagcgccctc tctcgcagcc tctcatcgac atgaacctcg tgattacttc
    11701 cttcctgttc taatgcctct gcgatagcct gaaggccatt catggccatc cacggacaat
    11761 gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg tgcttccagt aactctttat
    11821 ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc ggttgccaca ataagcctct
    11881 gatgaggcaa tgcttttgca gcagcgatca gttgactggt ggaaccgacc gcatccgcca
    11941 tatcgacaat agcttgtggt gattctggat gcaccagtat ggcagcatcc gggtattctt
    12001 cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg cacaatgcag gcaccctgcc
    12061 agcataaaat gtctccaccc gtctgttttt gcacgtaacg ccccagatgt ttgtcgggtg
    12121 cccagatgat tttttcaccc aaactatcaa gatgatcaat aagttcgacg gcaatgcttg
    12181 aagttaccac ccaatctgcg cgcgttttta ccgcagcaga agtgttggcg tagacgacga
    12241 cagtacgatc gggatgggca tcgcaaaatg cgttaaattc ttcaacaggg cagccgagat
    12301 ccagtgaaca ctcaacctga agtgtcggca tcagaattgt tttttccgga ctgagaattt
    12361 tggcggtttc tcccataaat ctcaccccag cgactaacaa agtagaagcg ggatgctttg
    12421 caccgaagcg cgccatttcc agagaatcag aaatacagcc accggtttct tctgccagtt
    12481 gttgaatttc gggatcggta tagtagtggg caaccatcac cgcattacgt tcttttagca
    12541 gacgttttat cttctcgcgg taatacgctt tttcatcaat gcttaacggc gtcggcttcg
    12601 gggggaaagg ataaatcgcc gtgtctggat caaacattac gctcatctta ccatctcgtt
    12661 ttacaggctt aacgttaaaa ccgacattag cgtaacattc gcttttatcg gtgtaatttg
    12721 ttttatatgc taaacaagat agtcgaatgg tggggaaaag tcactcgaat tacgcctaat
    12781 tccaccgacg tagagttgct tgcacagtaa gctctgacga tgtcatcagg tttacaaagt
    12841 gaaattgagg atgcctgaat agcaaaaaag cgcctttagg gcgctttttt acattggtgg
    12901 gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt caactgctct accaactgag
    12961 ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc cgaccccctc cttgtaaggg
    13021 aggtgctctc ccagctgagc taatcacccg atactgcgct ggatactaca aactacttcc
    13081 accgaaccca ccgtaattag tggtgggtcg tgcaggattc gaacctgcga ccaattgatt
    13141 aaaagtcaac tgctctacca actgagctaa cgacccggtg gtgggtgatg acgggatcga
    13201 accgccgacc ccctccttgt aagggaggtg ctctcccagc tgagctaatc acccgacact
    13261 acgctggata ctacaactaa ctatgttagt ggtgggtcgt gcaggatgac tcgacttcgt
    13321 ctcgcccttc ggcgccgttg ctgatgcaac gttatccttc acattttact accgcttcca
    13381 ccttagcgat tggtgggtcg tgcaggattc gaacctgcga ccaattgatt aaaagtcaac
    13441 tgctctacca actgagctaa cgacccactt tttcgttgct ttcggtttgt ttgatatccc
    13501 gtggcaacgg cggcatatat tactgatttc agacttgagc gcaacaaaaa tttcgatgca
    13561 gatcactcaa ctgcttatga ttcgcacgac acgaccagaa ataatgcgac ttctggtcgt
    13621 gtgttatgca ttacatcgcg ttcagacgtt tttgtgcctg tttagcgcca tcagtaccag
    13681 ggtatttact gataacctgc tggtatacgg ctttcgcttt tgcggtgtca cctttgtcct
    13741 gcatgatgac gccgacttta aacatcgcat cagcagcctt tggtgacttc ggatagtttt
    13801 tcactaccga agcaaaatag tacgccgcat catctttttt acccttgttg tagtttaact
    13861 gacccagcca ataattggcg tttggcaggt aagttgaatc agggtaattt ttgatgaaat
    13921 tctgaaatgc caccattgcg tcatcctggc gggatttatc ctgcaccagc gcaatagctg
    13981 cattgtaatc cgtgtttgca tcgccgcttt tcaccggcgc gccagcattc gcagtaccag
    14041 catcagctgt cggcgtcgtt gatgccgccg caccgctttg atcgctgctg gttgattgcg
    14101 ccgctgcacc accgctgctg aggctgtcga tctgcaacag gatctgcttt tgccgctcca
    14161 cgacctgatt cagttgatac tgattttcct gaatctgacc acgcagggaa tcaatatcgg
    14221 attgattatc agaaagttgt tgctggagtt gggttaaaag ctggctgtga gcattagaaa
    14281 tacgctcaag ttgagtgacg cggtcttcga ccgagcctga gccgacacta ctgattggtg
    14341 cctgagcaaa agcggcccag ggggccgcta taccaaccag taacgacaga ctcaatagtt
    14401 gatgtctgaa gttactgctc atgcaattct cttagtaaac cagtaccgca cgacggtttt
    14461 tggagtatgc tgcttcgtca tgacccagta ctgcaggttt ttctttaccg taagaaacga
    14521 tggagatctg gtctgcagaa acgcctttac cctgcaggta catcttaacg gcgttcgcac
    14581 gacgttcacc cagggagatg ttgtattccg gagtaccacg ttcgtccgcg tgaccttcta
    14641 cggtgacttt gtaagacggg ttgctacgca ggaagtttgc atgtgcatcc agcatttgag
    14701 cgaagtcaga acggatatcg tacttgtcca gatcgaagta aacgatgttg ttctgctgca
    14761 gctgttgcat ttgcagacga gcctgctctt cggaagacat gttgccgttg ccgccgttcg
    14821 catccatacc agtgccagca cccagcatgc cttcgctgcc gtcattgctg gcgttcttgt
    14881 tggaagaaca tgccgcaatt gccataacag gcagagcaat catcagccct ttcagcactt
    14941 tgttcagttg catttcaatg attcctttac tattcaatta attattatca cagatacggc
    15001 gaccaggcag ggaatttgac ctgtccatca gttgccggaa gacgcgcttt gaaacgccca
    15061 tctgtagaaa ccaaattcag cacggatccc atcccctgag aagagctgta gattaccata
    15121 gtgccgttag gtgccagact tggcgtttca tccaggaacg tggacgacag aacttgtacg
    15181 cctcccgttg ccagatcttg tttggcaatg tgctgctgcc caccattgga gctgaccatt
    15241 accataaatt taccgtcgct gctgacatcc gcatcctggt tctgcgaacc ttcccaggta
    15301 atacgttgtg gcgcaccgcc gttgatattc actttataaa cctgcggacg accggcctgg
    15361 tcagaagtaa atgccaggtt ctggctatcc gggaaccagg tcggttcggt attgttactg
    15421 cgaccatcag tcacctggcg gatctgacca gaagccaaat ccattacgta caggttcaga
    15481 ctaccggttt tcgacaaggc gaatgccagt ttgctgccgt ctggcgagaa tgcaggtgca
    15541 ccgttgtgac gcgggaatga agccacctga cgtacagcgc catttgccag cgtctgaata
    15601 accagcgcgg aacgaccgct ttcgaaggtc acataagcca gtttagaacc gtctggtgac
    15661 cacgccggag acatcagcgg ctgcggtgaa cggtgaacga caaactggtt gtaaccgtca
    15721 tagtcagata cgcgcagttc atacgggaac tgaccgccgt tggtctgaac aacgtaggca
    15781 atacgggtac ggaacgcacc tttaatgccg gtcagttttt caaacacttc atcactggcg
    15841 gtatgaccag cataacgcag ccactgcttg ttcactttgt acgagttctg agcaagtaca
    15901 gtacccggtg cgccgccagt gtcaacaagt tgataagcaa cattgtaaga accatccgga
    15961 ttcggagtga cctgaccgac aactacagcg tcaatgccca gtgcggacca tgcagctggt
    16021 tgtacttcct gcgcactacc cggctgctgt ggcagacgag cgcgatctaa cggattaaat
    16081 ttaccgctgt tacgcaagtc agcagcaacg atgccgccaa tatcttcagg tgccgcacca
    16141 ggccccgccc actggaaagg aacaacacca ataggacgac cggaatctac accgctgtcg
    16201 atcacaatgc ggacttcagc atgcagaact gatgcccaca gtatgagaaa accaaatgct
    16261 actcgtaatg cctgcttcat catatctccc ttatctggac cgatggccca cgataattta
    16321 gcagaatgtt aacaaactaa aatacacaaa actaccagaa ccccgtggca accggtgcct
    16381 gatgttgacc gtccgaacag tcaacatcgc gattacggtt tgaagtccaa tggcgcgttt
    16441 ttgaacactt catatactgc ctggcttggt ggtttcggga tcttcgcaag tttagctgct
    16501 gccaacgcag cctgacaaag tgcgggatcg ccaccttcag gtttgatatc cagtaacata
    16561 ccatcgggtg ccagttttat gcgcagcgta caggttttgc ctgcatagga cgatgcgtca
    16621 tagaacttac tttcgatagc agatttaatc tgcccggcat agttattgat atcggcccct
    16681 gatgcgccat tgtttttagt attaccactc ccggcaggcg aagcattgtt ccctttcgcc
    16741 cctccccccg ttttcggtgc attcttacca gagcttagct caccgaaaat atcatctgcc
    16801 tctgcggcag cttttgctgc agcggctttt tcagctgctg cttttgccgc tgccgctttt
    16861 ttgtctgcgg cggctttttc tgccgctgct ttcttatcag ctgctgcctt atcagcagcc
    16921 gctttttctg ctgctttttt gtcggctgca gctttctctg ctgccgcttt cttatcagct
    16981 gcagcctttt cagcagccgc tttcttctca gcttctgcct tggctttttc agcagcttca
    17041 gttgccgctt tctttcttgc ttcagctgca gctgcttctg ccgcttccgc tttcttcttc
    17101 agtgccgcag cggctacctc ggcttttttc tgcgcttcgg ctgcggcttt ggcggcttct
    17161 gcttctgctt ttttcttcgc gtctgcagcc gctttcttcg cagcttcttc cgcagcttta
    17221 gcatctgccg ccgctttcgc tgccgccact tcagcttgct tctgctttaa ctcggcctgt
    17281 tttgcggctt cttcagcctg ctttttctgc tcctgagccg ctaaccgctc tttctcaagt
    17341 tgcttcaggc gttcctgttc agccgcttgt ttctcacgca gttcttcagc agcctgctgt
    17401 tccttcatct tgcgctgttc atcagaacgc ttcgcgcttg attcctggct ttgcatgcgt
    17461 ttgtactgct caactaccgc acctgaatca accatgacag cgtcgatgga cgaaccaccg
    17521 ccgcctccgg ctgaagcttc tatattctca tcgaacgaac tccagatcag cgccgcaaat
    17581 aagatgacat gcagcactgc tgaaataatt atcgcccgct tgagcttgtc gttttgttcg
    17641 gttgcctttg acactctcgg tttccaaaaa ctgttcgcct gttacccgct ctctttcaag
    17701 caagggaaac gcagatgttt agataggctg cgtcattaaa ccaaccgatt tcacacccgc
    17761 actatgtaac aagttcagtg ctttaattat ttcatcgtaa ggcacatctt ttgcgccacc
    17821 gatcagaaag accgttttcg ggttggcctt gaaacggctg gacacttccg ccaccacctg
    17881 ctctggtggt aaacgctcca ggcgatcttt ctcaaccacc acggtgtact gaccaatacc
    17941 agacacttca acaatcactg gcggattatc gttactgctc accgcctgtg attcagtagc
    18001 gtctggcaga tcgacctcca cgctctgggt gatgatgggc gctgtcgcca taaagatcag
    18061 caacagcacc agcagtacgt ccagcaacgg tacaatgttg atttcggact tgagatcgcg
    18121 acgacctcgt ccacgcgctc tggccatggc ttaccccttg ttgctctcgc taacggtaaa
    18181 cgcctggcgg tgcagaatcg cggtaaactc ttccataaag ttgtcgtaat tcagttccag
    18241 tttgtttacg cgctggttga gacggttata agccattacc gccggaattg cggcgaacag
    18301 accaattgcc gtcgcaatca acgcttctgc gataccgggc gcaaccattt gcagtgttgc
    18361 ttgttttacc gccccgaggg cgataaaggc gtgcatgatc ccccagaccg taccaaacag
    18421 accaatatac gggctgatgg agccaaccgt accgaggaac ggaatgtgcg tttccagatt
    18481 ttcaagttca cggttcatgg agatacgcat agcacgcgac gccccttcca ctacggcttc
    18541 cggcgcatgg ctattggcac gatgcaggcg cacaaactct ttgaacccgc tgtaaaagat
    18601 ttgttccgaa ccagtcagat tatcccgttt cccctgactc tcttgataga ggcgagagag
    18661 ttcgattcca gaccagaatt tatcttcaaa cgcttcggct tcacgcgccg ctgcgttaag
    18721 aatacgggtt cgctggataa taatggccca ggatgcgatt gaaaaaccaa tcaaaatcaa
    18781 catgataagt ttaaccagaa ggctagcctt caggaacaaa tcaaggatat tcatgtcagt
    18841 cactgcttaa actccgcgac aatagacttg ggaagcgcac gaggcttcat tttgagtggg
    18901 tcaacgcaaa caaccagaac ctctgcttca ttcagcaaag tattctcggc gttgacaata
    18961 tgttgcgtga aaaccaaaga ggtgccacgc attgatgtta tttcagtctg tatttcgagc
    19021 atatcgtcga gccgcgcagg tgcgtaatat tccaccgtca ttttacgtac cacaaaggca
    19081 acgcgttcag ccatcagcgc ctgctgactg aagtgatgat gacgcagcat ctctgtgcgt
    19141 gctctttcat aaaaagcgac gtaactggcg tggtacacca caccaccggc atcggtatct
    19201 tcatagtaga cgcgaaccgg ccatcgaaac agcgttgtat tcactttaca tcccggtaat
    19261 gcaacaaaag ttagagcttt taaacgctgc tactatacgc gcgcgattga ggtttgggaa
    19321 tgagggcaag ttaagggagc gtaaaaattt atgggcctgg agcggcccat aattttttaa
    19381 agagaattct gatttaaaag aagaagaaaa tcagcccgac aatgagaaca atatcggcaa
    19441 gcaatgggca aaaaatccct tgccaaagaa ccttctgcgg acgaaagccc acgccgtgaa
    19501 tcacaccagc acagacggcc cacatcagca ataaaccatg ccagatttcc agttcactgg
    19561 tcttcgcggc aaaacgagat gggtcccaaa acatacatcc tgctaacaga agcgccatca
    19621 cgaaggaaag cgcccgcagg gggcgcttgt ccattaccgc atacaaggtc gcgataatct
    19681 tactcatcag atgtcttctt gaccggcttt gcctgattcg acgtgttcaa gcgccagcgc
    19741 ggtgattacc ccaaacgaac aggcaagaag cgttcccaga atccatgcga aataccacat
    19801 ttttagctcc ttacttagta cagagagtgg gtgttacgtt caatatcttc tttggtgata
    19861 cgaccgaaca ttttccagta acaccaggcg gtgtagagca gaatgatcgg taccagaacc
    19921 accgcaaccc aggtcatgac gttaagcgtc agctggctgg aagttgcatc ccacattgtc
    19981 agacttgcgt tcatcatggt gctggacggc atcacaaacg ggaacattgc gataccggct
    20041 gtcaggatga tgcaggccag cgtcagggag gagaacacaa acgcccacgc ggctttatcc
    20101 atacgtgcag tcaggatggt cagcagcggc agaaccacac ccagtgccgg aatagcccac
    20161 agaattggcg tgttgttgaa gttaaccagc catgcgccag cttcacgaac cacttcttta
    20221 ttcagtgggt tagaggctgc gtaatggtcc attgtcgatt tcacgacata accatcgata
    20281 ccgtacatca cccatacgcc agccagtgcg aaacagacca gtgtcaccag cgcagccacc
    20341 tgagccgttg cacgggtacg caggtgcagt tcgcccccgg tacgcatttg cagataggtt
    20401 gcgccctgag taatgatcat ccctacgctc accacgcctg ccagcaggcc gaacgggtta
    20461 agcaactgga agaagttacc ggtgtagtac agacgcagat attcatcaac gttgaacggt
    20521 acgccctgca acaggttacc gaacgctaca ccaattacca gcggcggaac gaagctacca
    20581 atgaagatgc cccagtccca catgttacgc cagcgggttt cttcaatctt ggagcggtag
    20641 tcaaaaccga ccggacggaa gaacaaagac gccagcacga ggatcatcgc cacatagaag
    20701 ccggagaacg cagcggcata gaccatcggc caggcagcaa agagtgcgcc gcccgcggtg
    20761 atcagccaaa cctggttacc gtcccagtgt ggtgcaatgg agttaatcat aattcgacgc
    20821 tcggtgtcgt tacgaccgag gaaacgggtg agcatgccca cccccatgtc gaaaccgtca
    20881 gtgactgcaa aaccaatcag cagaacgcca accagcagcc accagataaa acgcaatact
    20941 tcataatcga tcatttgacg actcctgtct tagcgtgccg gctgagtagt cgtggaagac
    21001 tgctcaaagt gatagcgacc ggttttcagg ctgcttgggc cgaggcgtgc aaacttgaac
    21061 attaagaaca attctgccac caggaacagg gtatacaggc cgcaaatcag caccattgag
    21121 aagatgagat cgcctgcggt cagtgacgaa ttcgccacag ctgtcggcag cacttcaccg
    21181 atagcccacg gttggcggcc atattcagcc acgaaccagc ccgcttctac agcaatccac
    21241 ggcagcggaa taccgtacag cgcggcgcgc agaagccatt ttttctcgcc aatgcggttg
    21301 cggatgacac tccagaaaga gagcgcgatg attgccagca gcaggaagcc acacgccacc
    21361 atgatacgga acgcaaagta cagcggcgct acacgcggga tggagtcttt ggttgcctgt
    21421 tgaatctgcg cttcagtcgc atcagccacg tttggcgtat agcgtttcag cagcagaccg
    21481 taaccgaggt ctttcttcat gctattgaac tggtcacgaa ccgcctggtc ggtagaacca
    21541 gaacgcagtt gttcgagcag agagtacgcc ttcatcccgt tacgaatgcg ttcttcatgc
    21601 tgcaccatca gctctttcag gccgataacc ggggtatcca cggaacgcgt tgcaatgatg
    21661 cccagtgcgt aagggatctg aatcgcaaat ttgttcgtct cctcttcctg atcaggaatg
    21721 ccgaacaaag taaaggcagc aggcgcaggt tgcgtttccc actcggcttc aatagcagcc
    21781 agtttggttt tctgcacgtc gcccatttcg tagccggatt catcacccag aacaataaca
    21841 gacagaacag cagccatacc gaagctggca gcgatagcaa aggagcgttt agcgaaggcg
    21901 aagtcacgac ctttcagcat ataccatgcg ctgataccga ggatgaacat cgcgccagtc
    21961 acataaccag acgctacagt gtgaacgaat ttcacctgag caaccgggtt aagcaccagc
    22021 tcggagaagc tcaccatctc catacgcata gtttcaaagt tgaaatcgga cgcgattggg
    22081 ttttgcatcc agccgttcgc aatcagaatc cacagtgcgg acaggtttga accgagcgcc
    22141 accagccagg tgacacacat atgctgaact ttacccagac gatcccaacc gaagaagaac
    22201 agacctacaa aggtggattc gaggaagaag gccatcagac cttcgattgc cagcggcgca
    22261 ccgaagatat cccctacata gtgggaatag taagaccagt tagtcccgaa ctggaactcc
    22321 atggtcagac cggtagccac acccagagcg aagttgatac caaacaactt gccccagaac
    22381 ttggtcatat ctttataaat ctgtttgccg gagaggacgt agaccgtttc cataatggcc
    22441 aggaggaacg ccataccgag cgtcagtggc acaaaaagga agtggtacat cgcggtcaag
    22501 gcaaactgta agcgcgacag ttcgactata tctaacatca tgactccttg ctcatcgcat
    22561 gaagactccg agagtgaacc ccgttagaaa gggtcacacg catgccccaa tagaaatttg
    22621 tttgaacact ttcatcatac catgtgaatg cacacaaaaa cggcgtaaag aaaggttgca
    22681 aacatgttaa taaaaactca aattgatccc acgtatatat tacgccgcaa aatccttaca
    22741 ataaacaggt ttttgttgaa gcaaatttgc atttttcgac ggtgatcaat ttatagctaa
    22801 attgccgcct ttcagccaat ttgatcgaga acaatttatc tctttttgat gcccatttcc
    22861 aagacttata cattgataaa tatcaatttc tacaaattat gttaatcatg tttcaacaag
    22921 gtaactttag tgacatttat gtttaaaatg tgtgaattat aagactgaaa ttagtttcgg
    22981 ttggcaatta aagataaaaa gagacgaagc aaaaacagca cggttgacct taattctttt
    23041 tttggtcact ggctaatacg acgcagagtt tcaggattat taataaaggc ataaatctcc
    23101 tccccgccga tattaacatt taattaactt ataattatat tataattaac tcacccgggg
    23161 ttattatttt gttaaaaata aagccacata tacgtagctt gagagtgaag ataaacgcaa
    23221 aattacctga tgcgctacgc ttgccagtgt ctacatcatc tctgcaatat attgaatttg
    23281 cctgcttttg tagaccgaat aaagcgttca tgccgtatcc ggcatgaaca aagcgccctt
    23341 tgtcaacaat ctggccgcgc atatgtggct tcggtttgat tgctattcag gcaagccggt
    23401 aactgaacgt ccgtgactgg ccgggtaaaa aaggccccga tagatttgaa ccttgatttt
    23461 cctcggtggt aatgtgttca tccatcatcg tttctgagca agaaatcacc tcgcgactga
    23521 aagcaacagt cgcatcacag gttgctgatt cagccggatt aaacagccgt aaaatcactt
    23581 cttgtcggtc ttcagctttc ttaagtgcgc ttatcaggca tcccactggg ggcattttca
    23641 acaaactata actttccggc acgttgaatc cggctttgtt gagcttcatc gcatcccatg
    23701 ggattttgtt gtaacactgt actggagtca gccatgctcg cgcctgctga gctacaccag
    23761 cggcggttgg cgtaccggta taactcaata aacttaggcg acaagaaagc agaccacgta
    23821 gttgtgagtc cgggactggc attttaattc ccgaaggccg cctaggcctt aaaagcagat
    23881 cttctttccc cagtaagccc acgccgcgca gcaacgtaat ggcaaaggtt ttcttctctt
    23941 caccgatgac ttcaaattca cgtagccctt cgctaaagac agccatgccg ttacgccctt
    24001 cctgtaaggc tgcatagttg agcatattcc ataccggaac cggcgcttct ttccagcctt
    24061 cttgctgcca gttgttcatt gcactgtcgt tcacggggcg cgttagcgaa ccaaactggg
    24121 tatctgccag aacactatcg gtgttaaaag atgtagggat caggacgcga aggcgatgat
    24181 cgtcagcctg gttatcaagg ttgatatcca catcaatgcg cctgttgtta tgactaagag
    24241 tgacaaccaa ctcgacacct actctgccag tggattgtct tgcgctgcgt tgttgcagat
    24301 tacgcggcac cgccatttca tagcggataa cagccctgct ctgccaggcc tcatgaataa
    24361 tctcgcattg cggtttcgca ttcgccgaag taattaccca ctcttctttt gcgggtgaat
    24421 agtcatattc atcaccatca tcagagcttt cttcaatttg caacacccgg tcatagcgca
    24481 caccgctatc tttatctacc agttgtagcg ttccatcctc attgagcgca atttgccaga
    24541 aagcattttc cagtatccct tcagcgtcac tttttgccgc aatcacgttg ccaggctgat
    24601 tcgcttcgat ataaagcgtg cgatagccca tagaagggac aatctggttg atctgtatat
    24661 caaactccat aaagggatcg taattaccgt aatgaactat ttgccgatcg attaggcctg
    24721 gatcgatctc acgggcatgg cgaataaaat acggtacagg ctgaccgcga tcgtctcgta
    24781 gattaaactg gctggcgcgc agccgcacag tggtgttgat cacttcttcc cgcggccagg
    24841 gcatcaggtt aaacaggacg agtttgtcgg cgtcgctctg cggcatgttg tcggcgattt
    24901 tacgcatata gaaacgcagc agattatccg ccatgtcttc agccagttcg aagcgggcga
    24961 cgatttcgcg atgaactttg tcactacagc agcagccgat gctgtcgtgg gcatgatttt
    25021 ttaagatctc tttccatatt ttctccagca agccgtggtg gtattcaaaa cccaacgtcc
    25081 aggccagcgt tgccagcggt tccagcggtt ccagcggttc cagcagatta acaatcttat
    25141 tttcaatacg tgcgtgggca actttgatat ccatgcgcgt ggaaccgatg gtgcgatgca
    25201 cgcgcatata tttgccatca ataaattccc ctttcagggt tgccagatta tctcgctgcg
    25261 cttcgatctt ctcaaatacc tcttcaaagc ggctcatcac aaactttcgt tgaggataaa
    25321 tttcgcgcag tttctccatc acttcgaaga tattttgctg caatggcatc tgatcatgcc
    25381 cattcggcag caaaatctct ttggttacag acgctttttc cagcacgtca aaataactgt
    25441 cgaggcgttt gcggaatccg ttttcgtccg caggtaagta cttaccgatg gcataaccca
    25501 acggcatcac ctgcgccgtc acttcgctac cgtcactgct ttgccacaaa aactcggttt
    25561 tatccgtacc gtggcgctca gaacatccac gccagaacat ggtgcaggta atgccaaatc
    25621 cattgtagat atgcggcagt tgcccggaca tgccaaagga atcgggtaaa taacctattt
    25681 tcatcggctc gccaaacgcg aggcagtcac gcattccgta catcagatta cggacgatgg
    25741 attccgcaga aacaatcgtg gtatcggtct gggtatacca ggggccgata atcaacttgc
    25801 cggcttctac cagtttcttc acgcggtctt tgttttccgg tttcaccgcg aaataatctt
    25861 cgaggatcgc cgtttgcccg tcgagtacgt aatatttgta ttcgttgtcc tgttccagtc
    25921 ggcacaggat ctcttccata ttattgacca gcagaatgcg tgactcttcg gtggtgaaat
    25981 accactcccg atcccagtgc atatgcgggg tgatgtgaac gcgagatacc gctttcatcg
    26041 tcgtttcctt tttgtttatt gcattacgcc atcagtcaga tagttgccat gcttaaccgc
    26101 gtgacgccgc cagatcagga gaattgcagt cgagattgca gcccccacca atgccgcacc
    26161 aaaccagcca attgccgcca taacaccgcc cgcgccatta tcatgaagta aaaagagcga
    26221 gaaaatgcca gcaccgggtg tcgaaaggcc gatattcatc gcaccgacaa tagcgcccgt
    26281 taccatagag cccagcacaa acgaaccaat aacccgcagc ggatcttcaa tcgccatcgg
    26341 gatcgcccct tcggtaatac ctgccagccc taacagccag gtggatttcc cggtctcaat
    26401 ttcaaactct ttaaacaggc gcggtgcgag catcgtggaa gcggttacgg taaatgccga
    26461 aaccattttg acggaggcga aaatggcata cgggccgtaa acgccgttcg ccattgcgcc
    26521 caggcagaat gcataagcgg ctttattcac tggccctcca aggtcaaagg aacacataaa
    26581 accgagaatg gcacccagca acagcgcgtt acttcctgac agaccgttca gccaggcggt
    26641 aagcgagtta ttgatccagg cgacaggttc ccccaccaca aacagcatca gactgcccgc
    26701 tcccaatgta ccgagcacca ggtagagata aaaagtcagg aagccattga atttactgct
    26761 aagacgcaag tgatttttca tccagcgtat caagtaaccg gctatcaatc cgccaacgac
    26821 cgcgccgaga aacccggagc cgatcatgtt ggcggcaagt ccagccgcaa agcctggcgc
    26881 taacgccggt ttatctgcca gcgaataggc ggtataggct gccagcaccg gtaccatcaa
    26941 aattccgagc agcccgccgc ccagtttgcg gtacatccac agccaggagt tttcttcatt
    27001 aaacagatct tgtagcccga agatttgcga cagtaatacc gcgaccgcca gcaccgtccc
    27061 ccccgcgaca atcaacggta cggcaaaaga gatcccgctc aacagtgcct gtttcagctc
    27121 cgttttgacg cttttcaccg gttgcgtatc ttgctgtacg gtacgcgtct catcgccacg
    27181 ctcgagggta agcgattgtt ggatcaacgc ttctgcatgg cgaatcggct cggcaacagg
    27241 cacagaaagc gcaggaatgc cgttaaaacg ctcactctcc ttgatggcga cttcagccgc
    27301 aaaaatacag gcagttgcac tattgagttg atccgccgtt aaacgccctt caataccgtt
    27361 agcaccttgc ttttcaacgt agacattcac gccgagtttg cgtccggctt tttccagata
    27421 ctccgcagcc atgtaggtgt gagcaatacc cgccgggcag gccgttacgc aaacgatagt
    27481 tggcgcgttg gaaaaagagg cagaaggttg cgtgcctccc ttgtcatcca gcgccgagag
    27541 caattcatca ggcgtcgtcg ccgactgtat acgcgcccga atctcatcat ccgccaggcg
    27601 cgtggtcagc gctgtcagca gttgcatgtg cgtagtaccc gcttcattgg ggggaatcgc
    27661 cagcaacacc actaaatcaa ccgcttccgg gccatccacg ctttcccact gaagcggctc
    27721 gctgagtgtg gcgaccgcaa acgccgcttc ttttaccgca gcagttttgc catgcggcac
    27781 agccaaccct tcacctaagg cggtcgggcc aaggctttca cggcgataca cttctttcag
    27841 aaattgctca gtactggaaa ttttccccag agcagcaaga cgttgagtca acgcgtgaat
    27901 ggcctcttca cggctggtaa agcgcgcatt cagacacaac gcatcgcggt gggtcagagt
    27961 cgtcaggttc atcatgatcc tcacttgatc gtttttatag tcgcgccagt gtgcccgata
    28021 aaacaatacc attgcgtaat caatttcact tgattgaaat tatttacatt gtatttgtat
    28081 tatttacagg atgaatttat cgggcataat ggctaaattc gtattaatga gatgctaaat
    28141 aatgggacac aaacccttat accgacagat tgctgatcgc attcgtgagc aaattgcgcg
    28201 tggcgagctt aaacctggcg atgcgttacc taccgaatcg gcattacaaa cagagtttgg
    28261 cgtcagccgg gttacggtgc gtcaggcttt gcgccagtta gtcgagcaac agatcctcga
    28321 aagcattcag ggcagcggga cttacgtcaa agaagagcgg gtcaattacg atatttttca
    28381 gttaaccagt tttgatgaaa aactgtcgga ccgtcacgtc gatacacaca gtgaagttct
    28441 gatatttgaa gtgattccgg ctgacgattt tcttcagcaa cagctacaaa tcaccgtgca
    28501 ggatcgtgtg tggcatgtga agcgcgtgcg ctatcgcaag caaaagccaa tggcgctgga
    28561 agaaacctgg atgccgcttg cgttgttccc ggatctcacc tggcaggtca tggaaaattc
    28621 gaaatatcac tttattgaag aagtgaagaa gatggttatc gatcgtagcg aacaggaaat
    28681 cattcctctg atgccaaccg aagagatgag ccgtctgctt aatatcagcc agacaaagcc
    28741 gattcttgaa aaagtatcgc gtggatattt agttgatggt cgggtgtttg aatatagccg
    28801 caacgctttt aataccgatg actataaatt taccctgata gcgcagcgaa aatcatcgcg
    28861 ataagcacaa aaaaggccat catacgatgg ccaaccatgt cgaaaccgac agcccttcgc
    28921 cattcctgta gagggaagcg gcgagggcta tttcttatta cagatattta tttcagaaca
    28981 gttttcagtg cttcaccgat atccgccagg ctgcgaacgg ttttcacgcc tgcggcttcc
    29041 agagcagcga atttctcatc cgcagtccct ttcccaccgg caatgatcgc acccgcgtgg
    29101 cccatacgtt tgcctttcgg cgcagtcaca ccagcgatgt aacccacaac tggcttggta
    29161 acatgctctt tgatgtacgc tgccgcttct tcttcagcgc taccgccgat ctcaccgatc
    29221 atcacgatcg cttcggtctg cggatctttt tcgaacattt cgagaatgtc gataaagtta
    29281 gagccaggga tcgggtcacc gccgataccg acacaggtcg actgaccgaa accgtaatcc
    29341 gtggtctgtt taaccgcttc ataggtcagt gtaccggaac gggaaacgat acccacttta
    29401 cccggtttgt gaatgtgacc cggctggata ccgattttgc attcacccgg ggtgataacg
    29461 cctgggcagt tcgggccgat catacgaacg cctgcttcgt ccagtttcac tttcacggtc
    29521 agcatatcca gcgtcgggat gccttcagtg atggtgataa tcagtttgat gcctgcatcg
    29581 atagcttcca gaatggagtc tttgcagaac ggtgctggta cgtaaataac agacgcggaa
    29641 gcgccagtag cagcaacagc ttcacgcacg gtgttgaaca ccggcaggcc gaggtgggta
    29701 gtgccgcctt tacctggagt tacgccacca accattttag tgccgtatgc aatggcctgt
    29761 tctgagtgga aagtcccctg gctaccggta aagccctggc agataacctt ggtgttttta
    29821 tcaattaaaa tggacattat ttcccctcca ctgcggcaac aacctgctga gctgcatccg
    29881 tcagaccttt tgctgcaata atattcaggc cgctgtcagc cagtttcttc gcgccgagtt
    29941 cggcgttgtt accttccaga cgtaccacga ccggtacgtt aacacccact tccgctaccg
    30001 cgccgatgat accgtcagcg atcaggtcgc aacgaactat accgccgaag atgttaacca
    30061 gaacggcttt cactttgtcg tcagagagga tgattttgaa cgcttcggtt acacgttctt
    30121 tggttgcgcc gccgccaacg tccaggaagt tagccggttc gccgccgtgc agtttaacta
    30181 tgtccatcgt acccatcgcc agacctgcgc cgttaaccat acaaccgatg ttaccgtcca
    30241 gcgcaacgta gttcagttcc cactgtgcag cctgtgcttc acgcggatct tcctgcgact
    30301 ggtcacgcat ttcgcgcaga tcaggctggc ggaacagggc gttgccatca gcgcccagtt
    30361 tgccgtcgag gcaaatcaga tcgccctgtt tggtgatgac cagcgggttg atttcgatca
    30421 gcgccaggtc gcgctccagg aaaatggtcg ccaggcccat gaagattttg gtgaactgct
    30481 gaaccagttt accttccaga cccagtttga acgccagctc gcgtccctga tacggcatcg
    30541 ggccagtcag cggatcaagc gcaactttat ggatcaggtg cggagtttct tccgccactt
    30601 tttcgatttc cacgccgcct tcggtggagg ccataaagac cacgcgacgg gaactacggt
    30661 ctacaacggc accgagatac agctctttag cgatatcggt cgccgcttca accagaatct
    30721 ggttaaccgg ttggccattg gcatctgttt gatacgttac cagacgcttg cccagccagt
    30781 tttctgcaaa agcacggatg tcttctttgc tgtttacaac tttcacaccg cccgctttac
    30841 cgcggccacc agcgtgaacc tgacatttca ctacccacgg accggcaccg atttttgaag
    30901 cggcttcttc tgcttcgcgc ggagtagtac aggcataacc caccggtgct ggtaagccat
    30961 agcgggcaaa cagttgtttt gcctgatatt catgtaagtt catgtgttct gtccatcctt
    31021 cagtaatcgt tatcttttaa accgtagacc gacaccctat ccacgtcggt ctacagtgca
    31081 ggtgaagctt aaactactac acgtccagca gcagacgcgt cggctcttcc agcaactctt
    31141 tgatcgttac caggaagccc acggattcgc gaccatcgat cagacggtga tcgtaggaca
    31201 gcgccaggta catcatcggc aggatctcaa cctgaccatt caccgccatc ggacgatctt
    31261 tgatagcgtg catacccaga attgcgctct gcggcgggtt gatgatcggc gtagacatca
    31321 gggaaccgaa cacaccaccg ttggtgatgg tgaagttacc accggtcaga tcttcaaccg
    31381 tcagcttgcc atcacggcct ttgactgcca gctctttgat tttcttctcg atgtctgcca
    31441 tgccgagggt atcgacatca cgcagaaccg gcgtcaccag gccgcgcggc gtagaaaccg
    31501 ccatgctgac gtcgaaatag ttgtggtaaa ccacgtcatc gccgtcgata gacgcgttca
    31561 cttccgggta acgtttcagg gcttcaacca ccgctttcac gtagaaggac ataaagccca
    31621 gacggatgcc gtggcgtttt tcaaacgctt caccgtactg cttacgcaga tccataatcg
    31681 gcttcatgtt gacttcgttg aacgtggtca gcatggcggt ggagtttttc gcttccagca
    31741 gacgctctgc cacacgctta cgcaggcgag tcatcgggac acgtttttca ctacgtgcag
    31801 ccagagccgg ttgcgccgcc ggagcagccg ctgccggagc agactctttc gccggggctt
    31861 tcgccagatg tttttccaca tcttcacgag tcagacgacc acccacaccg gtgcctttaa
    31921 tggcgctggc gtcgagattg tgttcagcca gcaggcgacg gatcgccggg cttaacgcat
    31981 cgttgttttg ctcttccaga gacgcctgct ggcgttgcgc cggagtggac gctttctctt
    32041 cagatttggc gctggtttct ttaccggcgc tgttgccttc acgcaggcga ccaaggatct
    32101 gacgagacgt taccgttgta ccttcatctt ccagaaccgc atccagaatg ccgtctgctg
    32161 atgccggtac ttccagtacc actttgtcag tttcgatttc taccagcact tcatcacgta
    32221 cgactgcgtc gccgggtttt ttatgccagg ttgcgacggt ggcatcggct acggattcag
    32281 gcaggtcagg gaccagaata tctacgctac tcattgtgta tcctttattt attcgacgtt
    32341 cagcgcgtca ttaaccagat cttgttgctg tttctggtga acggacatat accctaccgc
    32401 cggagaggcg gaggccgggc ggcctgcata acgcagagaa gccccaaacg gaatcacttc
    32461 acggaaatga tgctggctgc agtaccatgc gccctggttg agcggctctt cctggcacca
    32521 gacaaaatcc ttgacgtgag caaactgctg caacacttcc tgcatcgctt tatgcgggaa
    32581 cgggtagagt tgctcgatac gcacaatggc gacatcgtgt tgattgttct tacgacgctg
    32641 ttccagcagg tcgtaataaa ccttaccaga acacatcact acgcgcttca cgcccttcgg
    32701 atcaagttcg tcgatttcac cgatggctgg caggaaggtg ccgttcgcca gttcttcgag
    32761 gctggaaacc gccagcggat gacgcagcag ggatttcggc gacatcacga ccagcggacg
    32821 acgcatcccg cgcagcgcct gacgacgcag catgtggtaa acctgtgccg gggtagacgg
    32881 tacgcaaacc tgcatgtttt gctcagcaca aagttgcaga taacgttcca gacgcgcgga
    32941 ggagtgctcc ggcccctgcc cttcgtaacc gtgcggcagc aacatcacca gaccacacat
    33001 ccggccccat ttctgctcgc cagaggagat gaactggtcg ataaccacct gcgcaccgtt
    33061 ggcgaagtca ccgaactgcg cttcccagat ggtcagggtg cgtggttctg cggtggcata
    33121 accatattca aacgccagca cggcttcttc tgacagtacg gagtcccaga cacggaacgc
    33181 gccctgaccg ttatggatgt gttgcagcgg cgtgtaagtg gaaccgttag actggttgtg
    33241 gatcaccgcg tggcggtgga agaaggtacc gcgaccggag tcttcacccg acaggcgaac
    33301 cggaatgcct tcatcaacca gcgtggcgta agcgaggttt tccgcaccgc cccagtcgaa
    33361 cagtttctca ccggctgcca tcgcctggcg atcgccataa atcttggcaa cgcgagactg
    33421 catttcaact gcttctggta ccgtactgat acgtttagcc agttcctgca ggcgcttcat
    33481 ctcaactttg ttcgggtact cttcgtccca ttcgtggttg aggtacggcg accaggtgaa
    33541 agagtgcatg ttcatcggac gccactctgc cactacgcaa tcgccagcat ccagcgcatc
    33601 gcggtacagg ttaaccatct cggtggcatc ttccagcgtc gccacttttt cctgctccag
    33661 cttgtcagcg tagattttgc gcggtgtcgg atgttttttg attttctgat acatcagtgg
    33721 ctgggttgcg ctcggctcgt cggcttcgtt gtggccgtga cggcggtagc ataccaggtc
    33781 gatgaagaca tcacgtttaa aggtgttacg gaaatcgagc gccagacggg tcacaaaggc
    33841 aacggcttcc ggatcatccg cgttaacgtg gaaaatcggt gcctgaacca tcttaccgat
    33901 atcagtacag tacggtgtag aacgggcatc cagcgggtta gaggtggtga aaccaacctg
    33961 gttgttgata acgatacgta ccgtaccgcc aacttcataa ccacgcgctt tcgacatgtt
    34021 cagagtttcc tgaaccacgc cctgcccggt cactgcggcg tcaccgtgga tggtgattgg
    34081 cagcactttg ttgctgctcg gctcatcaag tctgtccaga cgggcacgaa cagagccgat
    34141 aacgaccggg cttacaatct caaggtgaga cgggttaaac gccagcgcca ggtgcaccag
    34201 gccgccatcg gtctggaagt cagacgagaa gcccatgtgg tatttcacgt cgcctgtgcc
    34261 gaggtgttct ttatgtttac cggcgaactc gtcgaacaag tcttgcggtt ttttacccag
    34321 cacgttcacc agcacgttca gacgaccacg gtgcgccatc ccgagaacca cttcgcgggt
    34381 gccgctgttg ccagcgtggc ggatcatctc tttaagcatc gggattaacg cgtcaccgcc
    34441 ttccagcgag aagcgttttg cgccagggaa ttttgcgccg aggtaacgct caaggccttc
    34501 agcggcggtc agttcgctta agaagcgttt tttctcttcg ctattgaaag tcgcgcgacc
    34561 agactcgata cgctgttgga tccagcgttt ttcttcggtg ctggtgatgt gcatatactc
    34621 ggcaccaatc gggccgcagt aggtttgctt gagggcggaa ataagctcgc cgagtttcat
    34681 ggtttctttg ccgctggcaa atgaaccgac gttgaaggtt tcctggaagt ctgcttcggt
    34741 cagatcgtgg aaagacggat ccagatcggc cactttatct tgctgccaca gtcccagcgg
    34801 atctagattc gcatgctggt gaccacggaa gcggtatgcg ttaatgagct gcaggacttt
    34861 aacctgcttc acattggtgt cagggtcgga gatcgttgaa gagtaacgtg aagcgtcttt
    34921 cgccaggcgg cggaaatatt cacgcgtttg agagtggaat tgatccggtt tgactcccgt
    34981 accaggtaac tgctggaacg tcgaacgcca gttagcgtca accgagtcag gatcggttaa
    35041 gaagtcttca tagagctgtt ctatccagct ctggtttgcg ccagagaggt aagaagagtc
    35101 caaccaggct ttcaaagcgc tgttctgcat cgtgatccct taagcatctt ttttatgctt
    35161 acttcgccgt ggatactacc acgcacagtg atgtgcgtgc cggggttcac ttgcgagctc
    35221 ttgtctacgt agtggctcgc gaaggaacct ttaaaaactg tcttagtgtc aggggcagtt
    35281 tttaaagatt tcctgcattt ctttgttacg cctgatgcgc tgcgcttatc aggcctgcgg
    35341 gtaatgactt gtaggccgga taaggcgttc acgccgcatc cggcactggt tgcctgatgc
    35401 aacgcttgcg cgtcttatca ggcctacagt ttacgcatta cgttgcaaca acatcgactt
    35461 gatatggccg atggcgcgcg tcgggttcag ccccttcgga catacactga cgcagttcat
    35521 gatgctgtga cagcggaata cgctgaatgc atcgctcaaa ccgtcgaggc ggctgtcagt
    35581 ctcggtatca cggctatcga tcaggaaacg atatgccgct aacaagcctg ccgggccgat
    35641 aaacttatcg ggattccacc agaaagacgg acaagaggtt gaacaacatg cgcagagaat
    35701 acattcatac agcccgtcga gtttttcgcg ctgctctggc atctgtaaat gctcgcgagc
    35761 tggcggattt tgtccattat tcaacaggta aggcttaatt ttctcatatt gcgcatagaa
    35821 ttgtcccatg tctaccacca aatcgcggat caccggtaaa cctggcagcg ggcgaatcac
    35881 aatcttcttg cccggctggt tgagtgccga aatcggggta atacaggcca gaccattctt
    35941 gccgttcatg ttcagaccgt cggaaccgca cacaccttca cggcaggagc ggcggaacga
    36001 caggctggga tctttctctt tcagctggat aagcgcatcc agcagcatca tgtcgcgacc
    36061 ttcttccgct tccagggtgt aatcctgcat acgcggagca tcatcaacat ccgggttata
    36121 gcgataaatt gaaaactcga gtctcatttt cctgtctccg cattagtaag tacgaatctt
    36181 cggcgggaat gccgggcgca gtttcggttc catgttgacg cttcggcgcg tcatggattc
    36241 cgactcaggc agatacaggg agtggcacag ccagttttca tcatcacgat ccgggaagtc
    36301 gaagcggcta tgtgcgccac ggctttcggt acggaagttg gcagaaacag ccgttgcata
    36361 cgccgtttcc atcaggttat ccagttccag gcactcaacg cgctgggtgt tgaactcgct
    36421 ggaagtgtca tccagacggg catttttcag acgctcgcgg atcactttca actgctcaag
    36481 ccctttcgcc atcgcatcac cttcacggaa gaccgagaag ttatgctgca tacattcttg
    36541 cagcgcttta cggatcgcca ccggatcttc accgttacga ttattgttcc accggttcag
    36601 gcgatccaga gacgcttcaa catcagactc gctggcatcg cgcagtgcgc cctgctcggc
    36661 gatagactct tgcagatgca gacctgccgc gcgaccaaag accaccaggt ccagcagcga
    36721 gttgccgccc agacggttag cgccgtgtac cgatacacaa gcgatttcac caacggcaaa
    36781 cagtcccgga acaaccacat cttcgccttt ctcattcaca gtcagcgcct gaccggtaac
    36841 tttggtcgga ataccgccca tcatgtagtg acaggttggg ataaccggaa tcggctcttt
    36901 caccggatcg acgtgagcga aggtacggga aagctccagg atacccggca gacgggattc
    36961 gagaacttct ttacccaggt gatcgagttt cagtttcgcg tgtggccccc acggaccatc
    37021 acagccgcga ccttcacgga tttcaatcat gatggaacgc gccaccacat cacggcccgc
    37081 caggtctttg gcgttcggcg cataacgctc cataaagcgt tcgccatgtt tgttcagcag
    37141 ataaccgcct tcaccacggc aaccttcggt gaccagtacg cccgcaccgg caatgccggt
    37201 cgggtggaat tgccacattt ccatatcctg caccggtacg cctgcgcgga tagccatgcc
    37261 gacaccgtcg ccagtgttaa tgtgggcgtt agtggtggac tgataaatac gccctgcccc
    37321 gccagtcgcc agcacggtag cgcgggcttt gaaataaacc acttcaccgg tttcgatgca
    37381 cagtgcggta caacccacca ccgcgccatc ctggtttttc accagatcca gcgcatacca
    37441 ctcggagaaa atggtggtgt ggtttttcag gttctgctga taaagcgtgt gcaacagtgc
    37501 gtgaccggta cggtcagcag ccgccgcagt gcgtgccgcc tgctcgccgc cgaagttttt
    37561 cgactgaccg ccaaacggac gttgatagat acgaccatca tcgagacgcg agaacggcag
    37621 gcccatgtgt tcgagttcca gaatcgcttc cggcccggtt ttacacatat attcaatcgc
    37681 gtcctggtca ccgatatagt ccgacccttt tacggtgtcg tacatatgcc attcccagtt
    37741 atcttcatgg gtattaccca gcgcaacggt aatgccgcct tgcgcagaaa cggtatggga
    37801 acgggtcggg aagactttag agagcagcgc acaggtctgg ccgctctggg aaatttgcag
    37861 cgccgcgcgc atacctgcgc caccggcacc aatcacaact gcatcaaatt ctctgactgg
    37921 caatttcatc acacccccca caccacaacg aatccataaa tcacgtaaac caccagtgca
    37981 acgacaatca ccagttgcag catcaggcgc aaggccagcg gtttaacgta gtcggtcaac
    38041 acctgccaca tgccgatcca ggcatgaatc aagatagaaa acagcgccag cagggtgaac
    38101 actttggtga acgcagaggc gaagaaaccg atccagactt catatgtcag ttcgccactg
    38161 gtggcgaaaa aaccgaccat ataaatgatg tagagcgtca ggacgatagc ggtagcgcga
    38221 acgaggatga aatcatgtac gccattgcgt cctaatgcgg aggcgttgct taccatacga
    38281 ggactcctgc gagaagtgaa agcacgacag taataacaaa ggagattttg gcggagcgtt
    38341 tacccgcttc gaatgtttct tccagatagc caaaatccat catcatgtgg cgaataccta
    38401 cgacgacgtg atacgccaga gcggtaagga tgccccacat gataaatttg acgaagaagc
    38461 tgcccataat cgcggaagct tgctcgaaac cttcagggga agagaggctg gtacccagaa
    38521 gccacagcag gatgcccact gcaacaaagg tgatcacacc ggaaacgcga tggagaatgg
    38581 acgctatcgc cgtgatgggg aaccggatgg tctgtaggtc cagattaaca ggtctttgtt
    38641 ttttcacatt tcttatcatg aataacgccc acatgctgtt cttattattc cctggggact
    38701 acgggcacag aggttaactt tctgttacct ggagacgtcg ggatttcctt cctccggtct
    38761 gcttgcgggt cagacagcgt cctttccata actgcgcgtc atgcaaaaca ctgcttccag
    38821 atgcgaaaac gacacgttac aacgctgggt ggctcgggat tgcagggtgt tccggagacc
    38881 tggcggcagt ataggctgtt cacaaaatca ttacaattaa cctacatata gtttgtcggg
    38941 ttttatcctg aacagtgatc caggtcacga taacaacatt tatttaattt ttaatcatct
    39001 aatttgacaa tcattcaaca aagttgttac aaacattacc aggaaaagca tataatgcgt
    39061 aaaagttatg aagtcggtat ttcacctaag attaacttat gtaacagtgt ggaagtattg
    39121 accaattcat tcgggacagt tattagtggt agacaagttt aataattcgg attgctaagt
    39181 acttgattcg ccatttattc gtcatcaatg gattctttac ctgcaagcgc ccagagctct
    39241 gtacccaggt tttcccctct ttcacagagc ggcgagccaa ataacaaacg ggtaaagcca
    39301 ggttgatgtg cgaaggcaaa tttaagttcc ggcagtctta cgcaataagg cgctaaggag
    39361 accttaaatg gctgatacaa aagcaaaact caccctcaac ggggacacag ctgttgaact
    39421 ggatgtgctg aaaggcacgc tgggtcaaga tgttattgat atccgtactc tcggttcaaa
    39481 aggtgtgttc acctttgacc caggcttcac ttcaaccgca tcctgcgaat ctaaaattac
    39541 ttttattgat ggtgatgaag gtattttgct gcaccgcggt ttcccgatcg atcagctggc
    39601 gaccgattct aactacctag aagtttgtta catcctgctg aatggtgaaa aaccgactca
    39661 ggaacagtat gacgaattta aaactacggt gacccgtcat accatgatcc acgagcagat
    39721 tacccgtctg ttccacgctt tccgtcgcga ctcacatcca atggcagtca tgtgtggtat
    39781 taccggcgcg ctggcggcgt tctatcacga ctcgctggat gttaacaatc ctcgtcatcg
    39841 tgaaattgcc gcgttccgcc tgctgtcgaa aatgccgacc atggccgcga tgtgttacaa
    39901 gtattccatt ggtcagccat ttgtttatcc gcgcaacgat ctctcctatg ccggtaactt
    39961 cctgaatatg atgttctcca cgccgtgcga accgtatgaa gttaatccga ttctggaacg
    40021 tgctatggac cgtattctga tcctgcacgc tgaccatgaa cagaacgcct ctacctccac
    40081 cgtgcgtacc gctggctctt cgggtgcgaa cccgtttgcc tgtatcgcag caggtattgc
    40141 ttcactgtgg ggacctgcgc acggtggtgc taacgaagcg gcgctgaaaa tgctggaaga
    40201 aattagctcc gttaaacaca ttccggaatt tgttcgtcgt gcgaaagata aaaatgattc
    40261 tttccgcctg atgggcttcg gtcaccgcgt gtacaaaaat tacgacccgc gcgccaccgt
    40321 aatgcgtgaa acctgccatg aagttctgaa agagctgggc accaaagatg acctgctgga
    40381 agtggctatg gagctggaaa acatcgcgct gaacgacccg tactttatcg agaagaaact
    40441 gtacccgaac gtcgatttct actctggtat catcctgaaa gcgatgggta ttccgtcttc
    40501 catgttcacc gtcattttcg caatggcacg taccgttggc tggatcgccc actggagcga
    40561 aatgcacagt tacggtatga agattgcccg tccgcgtcag ctgtatacag gatatgaaaa
    40621 acgcgacttt aaaagcgata tcaagcgtta atggttgatt gctaagttgt aaatatttta
    40681 acccgccgtt caaatggcgg gttaattttt atatgcctaa acacaaaaaa ttgtaaaaat
    40741 aaaatccact atcggacatt catatattct aaaaaaaaat atatcggtat aaattataaa
    40801 caaatcgatg atttttaatc atctatcttc ctctcagtta catgaataaa ccaccaaata
    40861 tagcgcaccc ctataatttc caatacttta ttcatcaaac atttaattca taccatcaat
    40921 taattacttt tacatttaat aaaattaata atatttttaa gaaaaactaa aaacattcga
    40981 caatcaaata agatattctt gcaccgatat acttttttct tttctgttaa aggactcagt
    41041 tatgtttaaa ggacaaaaaa cattggccgc actggccgta tctctgctgt tcacagcacc
    41101 tgtttatgcg gctgatgaag gatccggtga aattcacttt aaaggtgaag ttattgaagc
    41161 accgtgtgaa atacatcagg atgatattga taaagaggtt gaactcggtc aggtgaccac
    41221 cagccacatt aatcagtcac atcacagcga tgccgttgct gtcgacctgc tcttagtcaa
    41281 ctgtgatctg gaaaactcca gcaacggttc cggtggcaag atttctaaag ttgcagtcac
    41341 ctttgatagc tcagcgaaaa ccaccggcgc agatccgatt ctcaacaaca ccagcacggg
    41401 tgaagccacc ggcgtcggcg tacgtttaat gaataaagat caaagcaata tcgttttagg
    41461 caccgctact ccagatatcg acctgctccg acctccagcg aacaaacgct gaatttcttt
    41521 gcctggatgg aacaaattga tcaggctaca cctgtaacgc caggcgcagt tacggcgaat
    41581 gcaacctacg tgctggatta taaataagta ttactttggc ggcgctatcg ccgcctttca
    41641 taaaatattt atcaggtatg gacaccgtga atatttatcg actctctttt gtatcctgcc
    41701 tggtcgtggc gatgccttgc gcattggcgg tcgaattcaa tctgaatgtt ctcgataaat
    41761 caatgcgcga ccgcattgat atttcattat taaaggaaaa aggagtcatt gctcccggtg
    41821 agtattttgt tagcgttgcg gtaaataata accaaatcag taacgagcaa aagattaact
    41881 ggcacaaaaa tgacgataaa accattccgt gcatcaatga tttactggtc gataaatttg
    41941 gcttaaaacc tgaagtccgt cagtcattac cattgataaa tcagtgcgtc gattttagct
    42001 cccgacctga aatgctcttc aatttcgatc aagccaatca gcaactgaat attagtattc
    42061 cgcaagcctg gctggcgtgg tactcagaaa actggacccc accctccaca tggaaagaag
    42121 gtgtcgccgg tgtcctgatg gattacaact tgtttgccag cagctaccgc ccacaggatg
    42181 gcagcagcag cactaatctt aacgcctatg gaacagccgg aatcaacacg ggggcatggc
    42241 gcttacgcag tgattatcag ttgaatcata ctgatagcga tgataaccat gaacagtcag
    42301 gcgaaatatc acgtacctat ctttttcgcc cgttaccgca attaggttca aaattaaccc
    42361 tcggcgaaac ggattttagt tccaatattt tcgacggttt ttcttatacc ggcgcggcac
    42421 tggcaagtga cgatcgaatg ttgccatggg aactacgcgg ctacgcccca caaattagcg
    42481 gtattgcaca gaccaatgcc acggtgacga tcagtcaatc aggccgcgtc atttaccaga
    42541 aaaaagtccc accaggccca tttatcattg acgaccttaa tcagtctgtt cagggcacac
    42601 tggatgtcaa agtgacggaa gaagatggtc gggtgaacaa tttccaggtt tcagcagcat
    42661 cgacgccctt cctgactcgt cagggacagg ttcgctataa actggccgcg ggtcagccac
    42721 ggccctccat gtcacatcaa actgaaaatg aaaccttttt tagcaatgaa gtttcctggg
    42781 ggatgctgtc aaacacctcg ctgtacagcg gcctgctgct ttctggtgat gactaccatt
    42841 ctgccgcaat gggtattggg caaaatatgc tgtggcttgg tgcgctgtcg tttgatgtca
    42901 cgtgggccag tagccatttt gatactcagc aggacgagcg gggcttaagc taccgtttta
    42961 attacagcaa acaagtggat gccactaaca gcacgatttc cctcgccgct tatcgtttct
    43021 ccgatcgtca ttttcacagc tacgccaact atctggatca caaatacaac gacagcgatg
    43081 cgcaggacga aaaacagacg atcagcttat ctgtgggcca accgattacc ccactaaacc
    43141 tcaatcttta cgccaacctg ctacatcaaa cctggtggaa tgcagacgcc tccacgaccg
    43201 ccaacatcac ggcaggtttt aatgttgata ttggtgactg gagagatatc tctatttcta
    43261 cgtcattcaa tacaacccat tacgaagata aagatcgcga caaccagatt tacctgtcga
    43321 tttcgctccc cttcggtaac ggtggtcggg ttggctatga catgcaaaac agtagccaca
    43381 gcaccacaca ccgcatgtcg tggaacgata cgctggatga acgtaatagc tggggcatgt
    43441 ctgccggact gcaatccgac cgtcctgaca atggagccca ggtgagcggt aactatcagc
    43501 acctgagttc agcgggtgag tgggatattt ctggtaccta tgccgccaat gattacagtt
    43561 ccgtcagcag cagctggagc ggttctttca ccgcaaccca atatggtgca gcgtttcatc
    43621 gccgcagctc caccaatgaa ccacgcctga tggtcagcac cgatggcgtg gcagatattc
    43681 cggttcaggg caatctcgac tacaccaacc attttggcat tgcggtggtg ccgttgattt
    43741 ccagttacca gccttccacc gtggcggtga acatgaatga cttacccgac ggcgtaacag
    43801 ttgcagaaaa cgttatcaaa gaaacgtgga ttgaaggcgc gataggttac aaatcactgg
    43861 cttcccgttc cggtaaagac gttaacgtca tcattcgcaa cgccagcggt cagtttcctc
    43921 ccctcggagc ggatatccgc caggatgaca gcggcattag cgtggggatg gttggcgagg
    43981 aaggacatgc ctggttaagc ggagtcgctg aaaatcaaaa gtttaccgtg gtctggggtg
    44041 atagccagca ttgctcgctc catcttcctg aacatatgga agacaccgca aatcgcctga
    44101 ttttaccttg tcattaatta aggaaatgaa aatgacattt atgaaaggac tacccctttt
    44161 gttgttggtt gccagtctgt gcagccacgc tgcactacaa cccgatcgca ctcgtattgt
    44221 gtttaacgct aatgataaag ctaccagtct gcgggtggat aatcgtagcg ataaactccc
    44281 ctacctcgct tattcctggc tcgaaaatga aaaaggggag aaaagcgatg atcttctggt
    44341 cgccctgccg ccgatacaac gtctggaacc gaaagcaaca acgcaggtac ggatagtaaa
    44401 gcaagcctcg accacaaaat taccgggcga tcgcgaaacg ctatttttct acaatatgcg
    44461 tgaaattcct cccgccccgg aaaaaaacag cgaccatgcc gttctgcaag tcgccattca
    44521 gagtcgtatc aaggtgttct ggcgaccggc tgcattacgc aagaaagcag gagaaaaggt
    44581 cgaactgcag ttacaggtca gccagcaggg caaccaactg acactgaaaa atcctaccgc
    44641 gtattatctg accattgcct acctgggacg aaatgaaaaa ggcgttctcc ccggcttcaa
    44701 aaccgtgatg gttgcgcctt ttagtacggt caacacaaac accggaaatt atagcggcag
    44761 ccagttttat ctcggttata tggatgatta cggcgcgtta cgcatgacga cgctcaactg
    44821 cagcggacaa tgccatttac aagccgtgga ggcgaagaaa tgagtgctgg caagggagtg
    44881 ttgctcgtca tctgtctgtt atttctgccg ttgaagtctg cgcttgccct gaactgctac
    44941 tttggttcat ccggtggctc ggtagaaaaa tcagaagcga ttcaaccttt tgctgtacca
    45001 ggcaatgcca aactcggcga taagatctgg gaatctgacg atattaaaat tcccgtctat
    45061 tgtgacaaca ataccaacgg caactttgaa agcgaacatg tttacgcatg ggtaaacccc
    45121 tacccggggg tgcaggatcg ttattatcaa ctcggagtaa cttacaatgg cgttgattat
    45181 gacgccaacc agggaaaaag ccgcatcgac actaaccagt gtatcgacag taagaacatt
    45241 gacatttata cccctgagca gatcatcgcg atgggatggc agaacaaaat ttgctcgggt
    45301 gatcccgcta atattcacat gtcgcgtacc tttctcgctc gcatgcggtt atacgtcaaa
    45361 atacgagaaa tgccgccgca tgattatcaa agtacgctta gcgactatat cgtcgtgcaa
    45421 tttgacggtg ccggtagcgt taacgaagac cctactgccc aaaacctgaa atatcatatt
    45481 actggtctgg aaaacattca cgtgctggat tgcagcgtca atttttccat ttcaccggaa
    45541 acacaggtga ttgattttgg taaatttaat ttgctggata ttcgtcgcca cacaatgtcg
    45601 aaaacattca gtattaagac gacaaaatca caaaatgacc aatgcaccga cggatttaaa
    45661 gtcagttcct ccttttatac cgaagaaacg ttggttgaag aagataaagc attgttgatt
    45721 ggtaatggtc tgaagctacg tttgctggat gaaaacgcct cgccttatac cttcaataaa
    45781 tatgctgaat acgccgattt caccagcgac atgttggtct acgaaaaaac ctatacagct
    45841 gaactttcgt ccattgcagg tacccccatc gaggctgggc ccttcgatac agtggtgctt
    45901 tttaaaatta actataactg atttgccatg atgccctttg cagccggaaa gggcatctct
    45961 ttcagaaaat tgctgatttt ccccttttcc ttgattgctg ttattttttc ctagcccggc
    46021 tttctttatg ctgtatcgat acaccaaaga gaaaataatg agggagcgaa ggatgccagt
    46081 tttgcagtgg ggaatgttat gtgtgctatc acttctcctt tctattggtt tcctcgcggt
    46141 acatctcccg gcggcgctat tacttgggcc gatgatcgcc gggatcatct tcagtatgcg
    46201 cggaattact ctgcaacttc cccgctccgc ttttctcgcc gcgcaggcca ttcttggctg
    46261 catgattgcg caaaacctca ctggttcgat tctcaccacg ctggccgcca actggccgat
    46321 cgtgcttgct attttactgg tgacgctgct ttccagcgcc atcgtaggtt ggttattggt
    46381 gcgctatagc tcactgcctg gaaataccgg tgcctggggt tcttctccag gcggcgcagc
    46441 ggcaatggtc gctatggcgc aagattacgg cgcagatatt cgcctggtgg cgtttatgca
    46501 atatctgcga gtgctgttcg tcgcgggggc cgctgttctg gtcacccgca tgatgctggg
    46561 tgataacgct gaagcggtta accagcaaat cgtctggttc ccgccagtga gcattaatct
    46621 cctgcttacg attttgctcg cagtcgttgc cggtacggcg ggatgcatgc tgcgtcttcc
    46681 ttccggcacg atgctcatcc cgatgctggc gggcgcagtg ctccagtctg gtcagctcat
    46741 caccatcgaa ctaccggaat ggctgctggc gatggcgtat atggcaattg gctggcggat
    46801 tggtcttggt ttcgataaac aaatcttact gcgggcacta cgcccgctac cgcaaatcct
    46861 gctgtcgatt tttgctctgc tggctatttg tgcaggtatg gcgtggggac tgacccggtt
    46921 tatgcatatt gattttatga ctgcctacct cgccaccagt cccggcgggc ttgataccgt
    46981 ggcggtcatc gccgcaggga gcaatgccga tatggcgctt attatggcga tgcaaaccct
    47041 gcgcctgttc agtattttgc tgacagggcc agccattgcg cggtttattt caacctatgc
    47101 gccgaagcgg tcggcctagt gctggcagcc tggacaccag taaaacgggc gcgaggacaa
    47161 cgtggttttc tcaatgatgc cgccacagcg ttcgcacagt tcgccatcac gatgaaaaac
    47221 cttaaagcga aacagcgccc catgatgctt attctcatcc acctgccccc gcgtagcgta
    47281 agaaaaccga ggaatctcca gtaacgcgtg tgcgagtgca tccagttgcg ccgcattgag
    47341 atctttcgct ttatgatttc cagtcaaccc aacctgccag aggatctcca cccgcaaata
    47401 attgccaagc ccagccagaa acgcctgatc gagcagtaat cctgcaaact gacggttacg
    47461 aaagcgcggc gacaataacc gttcctttac cacctccggc gtcagattcg gatccagcac
    47521 atcgggacca acgcgttgta aaaacggatg cgtggtcagt tgttccggcc tcaacatctc
    47581 aatatccgag gcgctataaa gcagaatggt tttgtcagcc gtttgcagtt ttacccgcaa
    47641 tactcgcgtg gtctgcggct cttcgccggt atcaaccacg cgccagacgc cgtaaagctg
    47701 attatggctg tagagcgtta aaccgttggg aaaatgagtt aacaacgcct taccacgcgt
    47761 ttccacatgg gtaacgtgtt gaccgataag ttgtgattga taagttttta actgcgggaa
    47821 ggcaaaccag acatcagtta gtggtttgcc tttgatcgcc gcctccaggt tatccgctgc
    47881 acggcggatc tccgggcctt caggcatggt gatatccttt tttcattctt tattaagcgg
    47941 ggttatgctg cgacaacaat cccctttttg gcaaatgtag ctcgtaatcg gcgggcgaac
    48001 gccagagcgt gctcgccgtc gccatgcagg cagaccgttt gcgccgcgac cgttgcccat
    48061 tcgccggtga tacttttgac tctgccgtgt tgcaccatct ccagcgtttg cgccaacgcc
    48121 tgttcttcgt tttcaatcaa cgcgcctggc tgacttcgcg gcaccagcga gccgtcagcc
    48181 tgataaccgc gatcggcaaa cacttcctcg cgcgttgtca ggccatattg cttgcctgca
    48241 cgaatcagct cgcttcctgc cagcccaacg agaaccaatg ccggatcgca agcgtatacc
    48301 gctctggcga tggcgtctgc cagttgtgtt tctttcgccg cctggttgta caacatgccg
    48361 tgcggtttga catgacgcat tacgccgcct tgcgcacggg caatcgttgc cagcgcgcca
    48421 atttgataca gcgtctgggc gtaaacggtt tccggcggca gctgcatggc gctgcgacca
    48481 aaattttccc tgtcgggaaa actcgggtga gcgccaatcg cgacaccatt ttttattgct
    48541 tcacgcacgc aagcctgcat ggtttgcgca tcgcctgcat gaaatccaca agcaatattg
    48601 gcagaggaaa ccagcgttaa tagctctgcg tcgctggcgc agccttcgcc cagatcggcg
    48661 ttcaggtcaa ttttcattgt gcagccgcca cgctaattgt tcaaaataac gttgctgatc
    48721 ttgccgcgct ttcagtgcct cttccagtga acactggaca aaatgaatcg gctgaccgag
    48781 cggaatttgc gccagatggt acatatcagc ctcaatgata caggcaatac gtggataacc
    48841 accggtggtc tgtgcgtcgt tcatcaacac aatcggctgc ccgttatgcg gcacctgcac
    48901 cacgcccggt aacaaaccgt gagataacag ttcgcgatcg gtgatgcgtt ttaaaatttg
    48961 cccctgtaaa cgatagccca ttcggttact ttgcgagcta agctgccagg gcgaacgcca
    49021 gaatgcatcc tgcgaggcgc gatcgaactc atgatattcc ggccccggca aggcgcgaat
    49081 gcggttgccc cacagcagct gtttaacgcc ctgcgcttcc ataaaatcac gcttcgattt
    49141 gccaatcggg agtcggtcac catccttcag taaacggcct tccagcccgc caatccccac
    49201 tttgagatcg gtgctgcatg accccattac cggcggaaca tcaataccac ccgcgaccgc
    49261 cagataactg cgcatcccgt gctgcgggcg tttaagcgtt aaacgctggc ctgctttcat
    49321 cggcaatcgc cagccggtcc agacggcgtt atcatccaac cgcgcttcgc aacctgcacc
    49381 cgtcagggca aaccacccat cagtttcgaa ctcaactgtt aactgaccga gcgtgatctc
    49441 cagcgcgggg gcattggcgt cattacccac cagcaggtta gcaatgcgca gcgcaggcat
    49501 atccagtgcg ccacagtggc tgatacccga ctggcgaaaa ccgtgacgac cgccatcctg
    49561 cacagtggtg tacatgcccg cacgaataat cttcagcata ttccctcctt ctgcggtaca
    49621 aatcgcacgc tgtctcccgg acgtaataag atgggttcgt cacgcgccgg atcaaacagg
    49681 ctgagtgagg tatgaccaat caactgccag ccacccggcg ttgccagcgg ataaacacca
    49741 gtctgcggcc cgccgatccc gacagaacct gccggaacga gcaagcgcgg ttcagcgcgc
    49801 cgtggcgtgt gtagttgttc cggcaaactc ccgagatacg ggaagcccgg ttgaaaacct
    49861 aaaaaccaga ccacgtattc cacggaggag tgcaattcaa caacctgttt ttcgctcaac
    49921 ccgcaatacg ccgcgaccac cgccaaatcc ggtccgcctg caccaccgta aaccaccgga
    49981 atttcaataa agcgagactc aggttccagg gcctcgctct cctcccacca gcgttgcaaa
    50041 cgctctatgg catccagcgc cagcgactca ggattacgca aaatcaccgt gatattgttc
    50101 atgccgggaa tggcttcaac cacattcggc atatccacca gacgctgcgc cagtcgccag
    50161 atccgcttct ggctagccag cgtcaccggc ggttccagtt ccagcactac cgccgtttca
    50221 cctatcagat aacaacgcgc tcgttgcact ttcgatccct ctgattattc gttatgcagg
    50281 attaggaata tcaataaagg tcacatcaag atcggtattt tcattcagcc actcgctcaa
    50341 tgcgcgaata ccaccgcgtt cagtggcatg gtgacctgca gcataaaaat gcaatccctg
    50401 ctcgcgggct gaatgaatgg tctgttcaga aacttcgcca gtaataaaag catccacgcc
    50461 aaaacgcgcg gcgctatcga taaaactttg cccgccgccc gtgcaccagg cgacgcgctg
    50521 gaccacctca ggtccggtat cgccacacca taacggctta cgtcccagac gcgcttcaat
    50581 ccaggaggct agttccattc ccggcacagg catggtcagc tctccccacg gcaccaacgg
    50641 ctcaatttcg cccatgaccg tgatccccag taacgccgcc agctgtgcgt tattgcccag
    50701 ctcaggatgt gcgtcaagcg gcaaatgcca gccatacagg ttgatatcat tctccagcaa
    50761 cgtttttaaa cggttacgct tcattccgcg aatgaccgga gactctcctt tccagaagta
    50821 gccatgatgc acaatgaccg catccgcgcc cagacgcact gcctcatcga gcaaagcctg
    50881 gctggcggtg acaccggtaa caattttttg caccgtctct ttgccttcca cctgcaaacc
    50941 attcggcgcg taatcactaa tcgccgcgct gttcagtttt tcgttaatca gttgttccag
    51001 ttcggtgttt ttcatcttcg cgccttccgt attctgatag ctacaccgtt aacatagcgt
    51061 tacctcgcct gttcgtcgat agccaatctt ttgacatatt aagaaacaaa cccgacgcca
    51121 aaagacgcaa attgtttata tcatcacgct tcagctaatg tatatttatg caatcagaca
    51181 gcatattgca taaataatca gatctgtatc aaagttttaa ctttaaggaa acacaagcac
    51241 gccgcgcaac tattacgtga cgttgccgtc aattgtggaa ttattatgaa taaacacgca
    51301 tcacaaccgc gcgctattta ctatgtcgtt gcgctgcaaa tctgggaata ttttagcttt
    51361 tacggcatgc gtgccctgct gattctctat ctcaccaatc aactaaaata caacgatact
    51421 cacgcctacg agttatttag cgcctactgt tcgctggtgt atgtcacgcc aatcctcggt
    51481 ggctttttgg cggataaagt tctcggcaat cgcatggcgg tgatgctggg ggcgttgttg
    51541 atggcgatcg gtcatgtggt gctgggtgcc agtgagatcc atccgtcatt cctctatctg
    51601 tccctggcga ttatcgtctg cggctatggc ctgtttaaat ctaacgtaag ctgtctgctc
    51661 ggcgagctgt atgagccaac cgatccgcgt cgtgatggcg gtttctcgct gatgtatgcg
    51721 gcgggtaacg tggggtctat tatcgcacct atcgcctgtg gttacgccca ggaagagtac
    51781 agctgggcga tgggctttgg cctggcggcg gttggcatga tcgcgggtct ggtcattttc
    51841 ttatgtggca atcgtcattt cactcatacc cgcggcgtta acaaaaaagt actgcgtgcg
    51901 acaaactttc tcctgccgaa ctggggatgg ctgctggttc tgctggtggc aacgcctgca
    51961 ctgattaccg tgctgttctg gaaagagtgg tcggtatacg ccttaattgt cgcgactatt
    52021 attggcctgg gtgtactggc aaaaatttat cgcaaagcag aaaaccagaa acagcggaag
    52081 gagctggggc tgattgtgac gctcacctta ttcagtatgt tgttctgggc cttcgcacaa
    52141 cagggcggta gctcgattag cctttatatc gaccgcttcg ttaaccgcga tatgtttggt
    52201 tataccgttc cgaccgcgat gttccagtcg attaatgcct tcgcagttat gctgtgcggt
    52261 gtgttcctgg cgtgggtggt taaagaaagc gtcgcaggta atcgtaccgt gcgcatctgg
    52321 ggaaaatttg ctcttggcct tggcctgatg agcgccggat tctgcattct gaccttaagc
    52381 gcccgctggt ccgcaatgta tggtcactct tctctgccac tgatggtgtt aggcctcgcg
    52441 gtgatgggct ttgcggaact gtttatcgac ccggttgcca tgtcgcaaat tacgcgcatt
    52501 gaaattccgg gcgtgaccgg cgtattaacc ggcatctaca tgctgctttc tggcgcgatc
    52561 gcgaactatc tggcgggcgt gattgccgat cagacatcgc aggcttcgtt tgatgcttcc
    52621 ggggcgatca actactccat caatgcatat attgaagtat ttgatcaaat tacctggggc
    52681 gcactggcgt gtgtaggact ggtactgatg atttggctgt atcaggcgct gaaattcaga
    52741 aaccgcgcgc tggcgctgga gtcataatag gccagcaccg atgcctgatg cgacgcttga
    52801 cgcgtcttat caggcctacg tatttcctgc aatttattga atttgcacaa atttgtaggc
    52861 tggattaggc gttcacgccg catccggcat ctgggctcga ttgcctgacg cgtctgttat
    52921 ttccccttcc gcgccgcctc atacgctgcc aacgtttgta ctctcgcctc tttgtgctcg
    52981 actatcggtt gcggataatc cagcttcaca cctgctttct gcgcccactt ccacggctca
    53041 tgcaccgctt tccctggtac atcgcgcagc tccggtaacc atcgacgaat aaactcgccc
    53101 tcacggtcaa atttctcgcc ctgagttata ggattgaaaa tacgaaaata cggcgcagca
    53161 tcagtacccg tagaagccgc ccactgccag ccaccgttat tagctgccaa atcaccatca
    53221 atcagctgcg acatgaaata tcgctcgcct tcgcgccagt cgatcaacaa atctttcacc
    53281 agaaaactgg ctgtaatcat ccgtagccgg ttatgcatcc agccagtgct gttaagctga
    53341 cgcatagcgg catcaataat cgggtatccc gttttgcctt tctgccaggc ctgtaaatgt
    53401 gcgggattgc tttgccactg cacacgatcc gtccaggcaa taaatggacg atgtttacac
    53461 aacgaggggt aatacgtcat cagatgacgg tagaactcgc gccagatcag ctcactaagc
    53521 cagacactac cggccccacc gtccagcgcc tgcggctgtt ccgccaacaa gcgatgcaaa
    53581 cactggcgag gcgataaccc gcccgttgcc aagctggcgg acaaacggct ggtgccttcc
    53641 actgccggaa aatctcgttg ttgctcatac tctccagcac cgttctcgca aaactggcgt
    53701 aattgcgcaa tcgccgcttt ttcttccacc gggaaatgcg cagtatcgaa agactgacga
    53761 ggataattca gcgtaatgga tggcgcgggc tttatcgatc cgctactacg aacttttggt
    53821 gcagcgacgc actccggcat cccttcccgc agccgtttca gccaggcatt cttaaaaggc
    53881 gtaaagactt tgtacatctc gtgattgccg gtcatcaccg cccccggcgg caggatcacg
    53941 ctgtcatcaa atccttcaca caccacgtta cgcagcgctc tttcaacgtc cacatcccgc
    54001 gctcgctcat tcacctcata ctgatagtta taaaaaagat gagtaacgct gttttccgcg
    54061 cacacctgtt taactatttc gacactggcg acaaagtcat ccacttcacg gaacaataaa
    54121 ggaatacctt tttccgccag cgcgatttgt agcccattca gttgagcatt gataagttca
    54181 gcctgacgcg gcgacatgtt atgcgtctcc cactggcgtg gtgtagcgat atacagcgcc
    54241 agcacgcgtg cagacgaatt gcggcaggcg gcagccagtg cgagattatc gtgcagacgt
    54301 aaatcctggc gaaaccaggc cagatgggta gtcataaaac tcctgaatgg cgcaagttaa
    54361 tcacacgcgg ccaaagttcg aaataacgct gccctgaaag ccaggcgtca ggataaagcg
    54421 ccatatagtg tttgatacgc atcagcggcg caagaagtgg ttgctcgcca cggcgataac
    54481 tgtcgataag cgcagccagc tgctggcgtt ctgtggaatc aatatgcggg cgaaaataac
    54541 cctgaacgtg catcaaaacg ttggtgtgat cgcgcgggtt ggcgggatga gaaagcaaaa
    54601 caattacgcg ttggcggtac tggttgtaaa aatcatccag attttgccac tgatgaatat
    54661 cagccacaaa cgggccaagt ttgcggtact cgggctgaga atgggccaga aagacgagtt
    54721 tatagcggct gtgataatcg agaagcgccc cgcgcgttaa gccatgttct ttcagtcgat
    54781 gtaactcatg aagggcgaag atacggatta gggtgctgtc atcaaatcgt tgtagattca
    54841 ttactgctcc tgaacggtat acaggagtaa gtgtagacgt tggtaaatga tgtttgtgaa
    54901 gttaatccgc tagcagggca agattacgaa agcgcgctcc ccgcaagcaa ctaatcaagg
    54961 tagttaatca tggctgaaca cgatattacg ccagaagtca ccatttccaa aacacagcgc
    55021 cgctataaag tcggctatgt cagagcccgc caccaggacc gcaaaaccgg tatagcccgg
    55081 tattatagcc tgcaccttaa gggcgactgg ctgaaagagg ccggatttga taccggacgg
    55141 ggtgtcacgg tgaagatttc ggaggggggc ctgaccatca tcgctgacag tgacgaggtg
    55201 caggaactgc gggaggaact ttatcaggtt aaacaggtag ttaaggggat gcgggatggg
    55261 atgtttagtg tgctgaacga aagttaaata ttaaaaaagc tgggaagatt taaaaccttc
    55321 ctagcttttt attctgtctt attataatgt atactcatca tcttccgatg aatttataaa
    55381 ttgctccaag tttacatgaa gagtacttaa cctagttttt aactcattaa gtgaagcgat
    55441 aatttcatat tttccaacat aatcatatga tggatcgtat tcattatcta tgtcagaagc
    55501 taaaaatgat gatattcgta gtttaaattt agcttcatct ttattacatt cattggcata
    55561 aggtgaatgc cctatcatag catattcctc taatctaaaa ggataagatg gttttaattc
    55621 atcgatcaat gaaatttcaa tccatgttct atatccaggc attaatggac taatttcttt
    55681 gagcaaaacc tttccactta ataatgtatt ga
//
LOCUS       DADMEQ010000014        55423 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.014, whole genome shotgun sequence.
ACCESSION   DADMEQ010000014 DADMEQ010000000
VERSION     DADMEQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.014"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..373
                     /locus_tag="JNZ05_000861"
     CDS             <1..373
                     /locus_tag="JNZ05_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8564189.1"
                     /translation="IAGYYMKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQIL
                     NAIFLGILCGIGMLYFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHAL
                     FWLAIGMLGIAMICLLFIKDI"
     gene            484..1407
                     /locus_tag="JNZ05_000862"
     CDS             484..1407
                     /locus_tag="JNZ05_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY8564190.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVKGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(1411..2229)
                     /gene="nlpA"
                     /locus_tag="JNZ05_000863"
     CDS             complement(1411..2229)
                     /gene="nlpA"
                     /locus_tag="JNZ05_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HAY8564191.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVQLVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            2451..2744
                     /locus_tag="JNZ05_000864"
     CDS             2451..2744
                     /locus_tag="JNZ05_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564192.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(2785..3975)
                     /gene="nepI"
                     /locus_tag="JNZ05_000865"
     CDS             complement(2785..3975)
                     /gene="nepI"
                     /locus_tag="JNZ05_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HAY8564193.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4186..4638)
                     /locus_tag="JNZ05_000866"
     CDS             complement(4186..4638)
                     /locus_tag="JNZ05_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HAY8564194.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(4691..6025)
                     /locus_tag="JNZ05_000867"
     CDS             complement(4691..6025)
                     /locus_tag="JNZ05_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY8564195.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVVLAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            6200..7966
                     /gene="adeD"
                     /locus_tag="JNZ05_000868"
     CDS             6200..7966
                     /gene="adeD"
                     /locus_tag="JNZ05_000868"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAY8564196.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(8012..9403)
                     /gene="uhpT"
                     /locus_tag="JNZ05_000869"
     CDS             complement(8012..9403)
                     /gene="uhpT"
                     /locus_tag="JNZ05_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HAY8564197.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEVSEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(9541..10860)
                     /gene="uhpC"
                     /locus_tag="JNZ05_000870"
     CDS             complement(9541..10860)
                     /gene="uhpC"
                     /locus_tag="JNZ05_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HAY8564198.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(10870..12372)
                     /gene="uhpB"
                     /locus_tag="JNZ05_000871"
     CDS             complement(10870..12372)
                     /gene="uhpB"
                     /locus_tag="JNZ05_000871"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HAY8564199.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(12372..12962)
                     /gene="uhpA"
                     /locus_tag="JNZ05_000872"
     CDS             complement(12372..12962)
                     /gene="uhpA"
                     /locus_tag="JNZ05_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HAY8564200.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(13037..13327)
                     /locus_tag="JNZ05_000873"
     CDS             complement(13037..13327)
                     /locus_tag="JNZ05_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase isozyme 1 small subunit"
                     /protein_id="HAY8564201.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(13331..15019)
                     /gene="ilvB"
                     /locus_tag="JNZ05_000874"
     CDS             complement(13331..15019)
                     /gene="ilvB"
                     /locus_tag="JNZ05_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HAY8564202.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPSFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQLRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPVAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPLGAANTEMVGE"
     gene            complement(15125..15223)
                     /gene="ivbL"
                     /locus_tag="JNZ05_000875"
     CDS             complement(15125..15223)
                     /gene="ivbL"
                     /locus_tag="JNZ05_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HAY8564203.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            15788..15877
                     /gene="tisB"
                     /locus_tag="JNZ05_000876"
     CDS             15788..15877
                     /gene="tisB"
                     /locus_tag="JNZ05_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HAY8564204.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            16157..17341
                     /gene="emrD"
                     /locus_tag="JNZ05_000877"
     CDS             16157..17341
                     /gene="emrD"
                     /locus_tag="JNZ05_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HAY8564205.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYKRTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(17349..17846)
                     /locus_tag="JNZ05_000878"
     CDS             complement(17349..17846)
                     /locus_tag="JNZ05_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAY8564206.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMWVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(17843..18205)
                     /locus_tag="JNZ05_000879"
     CDS             complement(17843..18205)
                     /locus_tag="JNZ05_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HAY8564207.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(18195..18542)
                     /locus_tag="JNZ05_000880"
     CDS             complement(18195..18542)
                     /locus_tag="JNZ05_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HAY8564208.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            18651..19064
                     /locus_tag="JNZ05_000881"
     CDS             18651..19064
                     /locus_tag="JNZ05_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564209.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(19111..20577)
                     /locus_tag="JNZ05_000882"
     CDS             complement(19111..20577)
                     /locus_tag="JNZ05_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001665828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY8564210.1"
                     /translation="MTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNSAYTCSPVCT
                     PARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWHLDGHDYFGT
                     GECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETFTWAHRISNR
                     AVDFLQQPVRAEEPFLMVVSYDEPNHPFTCPVEYLEKYADFYYELGEKSQDDLANKPE
                     HHRLWAQAMLSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQRENTWVIYTSDH
                     GEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPTMMALADIEK
                     PEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLVLNLFTSDEL
                     YDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLRPWRKDARPR
                     WMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(20588..22303)
                     /locus_tag="JNZ05_000883"
                     /pseudo
     CDS             complement(20588..22303)
                     /locus_tag="JNZ05_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
     gene            22470..23363
                     /locus_tag="JNZ05_000884"
     CDS             22470..23363
                     /locus_tag="JNZ05_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8564211.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVLEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(23360..24174)
                     /locus_tag="JNZ05_000885"
                     /pseudo
     CDS             complement(23360..24174)
                     /locus_tag="JNZ05_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inactive 6-phospho-alpha-glucosidase"
     gene            complement(24174..25790)
                     /locus_tag="JNZ05_000886"
     CDS             complement(24174..25790)
                     /locus_tag="JNZ05_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY8564212.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            26085..26759
                     /locus_tag="JNZ05_000887"
     CDS             26085..26759
                     /locus_tag="JNZ05_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY8564213.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNGLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(26798..28459)
                     /locus_tag="JNZ05_000888"
     CDS             complement(26798..28459)
                     /locus_tag="JNZ05_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HAY8564214.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(28655..29083)
                     /gene="ibpB"
                     /locus_tag="JNZ05_000889"
     CDS             complement(28655..29083)
                     /gene="ibpB"
                     /locus_tag="JNZ05_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HAY8564215.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(29195..29608)
                     /gene="ibpA"
                     /locus_tag="JNZ05_000890"
     CDS             complement(29195..29608)
                     /gene="ibpA"
                     /locus_tag="JNZ05_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HAY8564216.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            29913..30245
                     /locus_tag="JNZ05_000891"
     CDS             29913..30245
                     /locus_tag="JNZ05_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAY8564217.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(30247..31420)
                     /locus_tag="JNZ05_000892"
                     /pseudo
     CDS             complement(30247..31420)
                     /locus_tag="JNZ05_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
     gene            31485..32503
                     /locus_tag="JNZ05_000893"
                     /pseudo
     CDS             31485..32503
                     /locus_tag="JNZ05_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(32464..33801)
                     /locus_tag="JNZ05_000894"
     CDS             complement(32464..33801)
                     /locus_tag="JNZ05_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8564218.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(33876..35024)
                     /gene="dgoD"
                     /locus_tag="JNZ05_000895"
                     /pseudo
     CDS             complement(33876..35024)
                     /gene="dgoD"
                     /locus_tag="JNZ05_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(35021..35638)
                     /locus_tag="JNZ05_000896"
     CDS             complement(35021..35638)
                     /locus_tag="JNZ05_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HAY8564219.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCAMATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(35622..36489)
                     /locus_tag="JNZ05_000897"
                     /pseudo
     CDS             complement(35622..36489)
                     /locus_tag="JNZ05_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
     gene            complement(36486..37175)
                     /gene="dgoR"
                     /locus_tag="JNZ05_000898"
     CDS             complement(36486..37175)
                     /gene="dgoR"
                     /locus_tag="JNZ05_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HAY8564220.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            37453..38109
                     /locus_tag="JNZ05_000899"
     CDS             37453..38109
                     /locus_tag="JNZ05_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564221.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(38155..38967)
                     /gene="yidA"
                     /locus_tag="JNZ05_000900"
     CDS             complement(38155..38967)
                     /gene="yidA"
                     /locus_tag="JNZ05_000900"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAY8564222.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMGNAIPSVKEMANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(39081..39488)
                     /locus_tag="JNZ05_000901"
     CDS             complement(39081..39488)
                     /locus_tag="JNZ05_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HAY8564223.1"
                     /translation="MIIMGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQS
                     GGLGAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASCLLAEQLPK
                     IIDALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(39719..42133)
                     /gene="gyrB"
                     /locus_tag="JNZ05_000902"
     CDS             complement(39719..42133)
                     /gene="gyrB"
                     /locus_tag="JNZ05_000902"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAY8564224.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(42162..43235)
                     /gene="recF"
                     /locus_tag="JNZ05_000903"
     CDS             complement(42162..43235)
                     /gene="recF"
                     /locus_tag="JNZ05_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAY8564225.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(43235..44335)
                     /gene="dnaN"
                     /locus_tag="JNZ05_000904"
     CDS             complement(43235..44335)
                     /gene="dnaN"
                     /locus_tag="JNZ05_000904"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAY8564226.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(44340..45743)
                     /gene="dnaA"
                     /locus_tag="JNZ05_000905"
     CDS             complement(44340..45743)
                     /gene="dnaA"
                     /locus_tag="JNZ05_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAY8564227.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLSLYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            46040..46120
                     /locus_tag="JNZ05_000906"
     CDS             46040..46120
                     /locus_tag="JNZ05_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564228.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            46350..46490
                     /gene="rpmH"
                     /locus_tag="JNZ05_000907"
     CDS             46350..46490
                     /gene="rpmH"
                     /locus_tag="JNZ05_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAY8564229.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            46507..46866
                     /gene="rnpA"
                     /locus_tag="JNZ05_000908"
     CDS             46507..46866
                     /gene="rnpA"
                     /locus_tag="JNZ05_000908"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAY8564230.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            46830..47087
                     /gene="yidD"
                     /locus_tag="JNZ05_000909"
     CDS             46830..47087
                     /gene="yidD"
                     /locus_tag="JNZ05_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAY8564231.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            47090..48736
                     /gene="yidC"
                     /locus_tag="JNZ05_000910"
     CDS             47090..48736
                     /gene="yidC"
                     /locus_tag="JNZ05_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAY8564232.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHRFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            48842..50206
                     /gene="mnmE"
                     /locus_tag="JNZ05_000911"
     CDS             48842..50206
                     /gene="mnmE"
                     /locus_tag="JNZ05_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAY8564233.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            50448..50522
                     /gene="tnaC"
                     /locus_tag="JNZ05_000912"
     CDS             50448..50522
                     /gene="tnaC"
                     /locus_tag="JNZ05_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HAY8564234.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            50743..52158
                     /gene="tnaA"
                     /locus_tag="JNZ05_000913"
     CDS             50743..52158
                     /gene="tnaA"
                     /locus_tag="JNZ05_000913"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HAY8564235.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            52249..53496
                     /gene="tnaB"
                     /locus_tag="JNZ05_000914"
     CDS             52249..53496
                     /gene="tnaB"
                     /locus_tag="JNZ05_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HAY8564236.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWSAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGARLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            53628..54803
                     /gene="mdtL"
                     /locus_tag="JNZ05_000915"
     CDS             53628..54803
                     /gene="mdtL"
                     /locus_tag="JNZ05_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HAY8564237.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            54778..>55423
                     /gene="yidZ"
                     /locus_tag="JNZ05_000916"
     CDS             54778..>55423
                     /gene="yidZ"
                     /locus_tag="JNZ05_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HAY8564238.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT        13613 a        14754 c        13934 g        13122 t
ORIGIN      
        1 aatcgcaggc tattacatga aacgtattgg caagcgacta ttaatgctca ttgctatcgt
       61 gagtggtatg tgtttttacg ccagcgtact catggcgacg actccggcgg ttgagctgga
      121 attgcaaatt ctaaatgcca tcttccttgg tattctctgt ggtatcggca tgctttattt
      181 tcaggacttg atgcctgaaa aaataggctc tgcgacaacg ttatatgcaa atacttcacg
      241 cgtcggctgg attatcgccg gctctgttga cggaattatg gttgaaatct ggagctacca
      301 tgcgttgttc tggctggcga tagggatgtt gggtattgcg atgatttgcc tgctgtttat
      361 taaagatatt tagctcacca gtctgggcta agaaaaatcg gcgtggcgca aacagagcca
      421 tcctcgccat ttttgctatc atgcctgcat acataaacga caaaacagta tgcagaggga
      481 aaaatgggtt ccaccagaaa ggggatgctg aacgttctga ttgccgccgt gttgtgggga
      541 agttcagggg tctgtgcgca atacatcatg gagcaaagcc agatgtcgtc gcagtttttg
      601 actatgacgc gtttgatatt cgccggtttg attctgctga cgctgtcatt tgttcatggc
      661 gataaaatct tttctattat taacaatcac aaagatgcca ttagtctgct gattttttcc
      721 gtggttggcg cgctaaccgt acagctcact tttttgctaa ccatcgaaaa atcgaacgca
      781 gccacggcaa cggtgctgca attcctctca ccgacgatta tcgtcgcctg gttctcactg
      841 gtacgtaaat cgcgcccggg cattctggtt ttctgcgcta ttttgacatc gctggtcggg
      901 acttttttat tggtgacaca cggtaatccg acgtcattat cgatctctcc tgccgcgtta
      961 ttctggggca ttgcctcggc atttgctgct gcattctata ccacctatcc ctcaacgctg
     1021 attgcccgct atggcacgtt gcctgtcgtc ggctggagta tgctgatcgg cggtctgatt
     1081 ctattgcctt tttatgccag acaagggaca aactttgtcg ttaaaggcag tttgattttg
     1141 gcgttttttt atttgatcgt cattggtacg tccctgacat ttagtctgta cctgaaagga
     1201 gcacaattaa ttggcggtcc aaaagccagc attttgagct gtgcagaacc attaagtagc
     1261 gcgctactct ctttgctgtt gctggggatc acgttcacat taccggactg gctgggaacg
     1321 ctgctgattc tgtcatcggt aattttgatt tcaatggatt cccgtcgccg cgccagaaaa
     1381 ataaatcgtc cggcgcggga tgagtgagaa ttaccagcca ggcaccgcgc caccgttaaa
     1441 gatggtttct gccgctttag cgacttcgga tgattgataa gattgcagaa attccttcac
     1501 gttttctgca ttcttattat cttcccgtgc caccaaaata ttcacatacg gcgaattttt
     1561 atcttcaata aatacactgt cgtgcaccgg agaaagcccg gtctgctgaa tgtaagtggt
     1621 gctgataatc gccacatcaa ctttgggatc atccagtacg cgcgggagct gcgccccctc
     1681 gagttccata atctgcaaat gacgaggatt atcggttata tccagcgcgg taggtaataa
     1741 cccttttccc tctttcagag taattagctt ctctttttgc aacagtaaaa gcgcacgacc
     1801 aaggttggtc gggtcgttgg ggatcgccac tgtcgcgcct tcttttattt gcgccacggt
     1861 tttgattttt ttggaataac ccgccatggg gaagacaaac gtatttccca ccgctaccag
     1921 tttataacca tgcgcctgat tatcttgttc aagaaagggt cgatgctgga agacgttcgc
     1981 atcaagttcg ccatgatttg tcgcatcgtt cggtagcaac gaaccgctaa atcctaccag
     2041 ttgaacatcg aggccatatt tctcttttgc caccttttta gcgacttccg cgacatcttg
     2101 ttctgcgcca tttataacgc caactttaat gtgttttgca tcgctgctac tctggtcgca
     2161 acctgccagc agaattccgg ccagcaataa tgcggcccct gtccgtagat gatgtgttgt
     2221 cagtttcatt tttttatcct tttattaatg attacctgac tatagcggta aggtcgctgc
     2281 ggtttaaaaa acgaaacgct atcgataaga ataaaaagga ataaaagtgg aatataaagt
     2341 ctggcacttc ttactgacca cgcaagcgcg tttcgtacag catgatgaga gcgatgaaag
     2401 caaactacac ttatgcttca ttcgatacac atttgtaaaa ggttgaatcc atgaaaccaa
     2461 cgacactact gcttattttc acttttttcg ctatgccagg catcgtatac gccgagtcgc
     2521 cttttagctc actgcagtcc gcaaaagaga aaaccacggt attacaagat ttacgtaaaa
     2581 tttgcacgcc acaggcgtca ttatcagatg aagcgtggga aaagttaatg ttgtcagatg
     2641 aaaacaataa acaacatata cgcgaggcca tcgtggcgat ggaacgtaat aatcagagca
     2701 attattggga agcgttgggt aaggtagaat gcccggatat gtaagaacag caacaactac
     2761 tgaccaacgc ttctttacct gatatcagga tttcttcatt ttcacctttg cagtaaccaa
     2821 caatgcagtc agcaacatca atgtgccgga caacatcaac ggcgaagtca gaccaatatt
     2881 atccagcgca taaccgccga ttgccgcgcc acaggtatta gcaagctgaa taaccgccac
     2941 ctgaatagac ccggcttttt ctgcctgatc ggccagcgag cgggtgatcc acgttgacca
     3001 gccgacggga accaatgcaa aagttagccc ccagataatc gccacgccgg tagcaacgat
     3061 tttatcgctt ccccacaacg tcagtaccaa cgcactcaca gccagtatta acggcgcgcc
     3121 tgctaaggcc agttttaccg aacgtttaag aatgaacgac gaaagcgacg taccaataaa
     3181 gctggcgata ccaaaactca acagcaccag cgttaagcca tccacgccga atcccgccag
     3241 gttcatatac accgggcgaa tatacgtgaa gaaagcaaac tgcccggcga aagacatgaa
     3301 gatggcgatc atccctgcca tcacacccgg acgttgtaat aagcggaaag tattttgttt
     3361 ctgatgcgag ggttcgcctg gcagtgaagg caatgatttg ataatccaga aaatacacag
     3421 cacgcccatc accgccgccg cattaaagac attgcgccaa ccgataagct cgcctaaaaa
     3481 actgcccaac ggcgcggcaa tcaccagcgc aatagaaacc gcgccgaaga tcaccgacag
     3541 cgccttcggc accgtacgcg gcggtaccag acgcatggtc agcgacgccg acatcgccca
     3601 gaacccgccc agcgccagcc ccagacaggc acgaccgatt aaaagcaaac tgaatgagtt
     3661 agcaaaggaa accagcaagc aggagagcgt cagcaaaacg gcaaacaaaa taacaacgta
     3721 gcggcggtca gtggcctgaa ttgtctgggt aataaacaaa ctggcaaaca ttgccacaaa
     3781 ggcggtcacg gtcaccgatt gcccggcaac cccttccgaa atgcctaaat cctgggccat
     3841 tggcgtcaac aaactgacgg gcaaaaactc aacgataatc agacaggcga cacaaaacgc
     3901 caccgagaaa acggctgacc agttcggtcg ggtgatggca tccgcgccgc ggttttcggc
     3961 aataaattca ctcatggtgt tacccgtggc atgtttcagg ggaaaagccg accactttaa
     4021 cattgaaatg tgtgacgcat ttaacgtttt tgcaactttt acggtgttgc ttgagctgga
     4081 gttaagatcg aaacggaaca gggcaaagat taccggatgc ggcgtgaacg tcttatccac
     4141 ccgacaggtc aggcgcggtc tgtaggctga taagacgaaa cggtttcaat gcaaccagtg
     4201 aacgccctcg gcaggctgaa agaacgcgtt atagcgcatc tgaaaagcat taatatcctg
     4261 catttcaggt tcggtttcac gcagaaaacc cagcgccagc ctgacctgag catcggtaat
     4321 cggcgcggaa aggcgggctt tttgtaaaat ccggtgccat tgcagcaggt tctgttcgct
     4381 atcatcgaca atgacaattt gccagataag caacacctgc gcggcgtttt gcaggtgcga
     4441 ctcatccggg cgatgcaaat aacgtaaaaa ttcgtcaccg gctgactttc ccatttgatc
     4501 gaccatcgac tccacgggta cgacatcgat attcagccga tcgctgagac ggcgaatcgc
     4561 ttttctggcc ccggatgtca gcacccgaaa accaaccaca aacactaccg ccagcgtggc
     4621 gagcattatc cagatcatga gttctcctgc gtaatcagaa ctcatgatat ctggaaaccc
     4681 tcgccagtta ctaatccacc agaataatct tcaatgcgaa cagagctgcg accaccacca
     4741 cacacaggtt cagatcgcgc cagcgcccgg tgcacacttt catgatgcag tacgacataa
     4801 agccgagtgc aatcccttcg gtgatcgaga aagtaaaggg catcatcacc gtggtaataa
     4861 acgcaggcac cgattcggta aaatcatccc agttaacgcg cgccaggctc gaagtcatca
     4921 gcacgccaac aaagattaac gctccagcgg ttgcgtaagg aggaaccatc gccaccagcg
     4981 gtgagaagaa cataactaac aggaacataa cgccgaccac gacagccgtt agccccgtgc
     5041 gaccaccgac tgccacacca gaagtacttt caatataggc agtaacagac gaggtaccga
     5101 taaatgcgcc tgccaccgaa ctgacgctat caacatacag cgccttattc atattgggga
     5161 atttaccgtt gctatctatt aagcccgctt tatcagttac accaattaat gttcctgatg
     5221 aatcaaatag gttgatcagc ataaaggaga aaatgatacc ggcgagttca agtgttaacg
     5281 cgccgctcaa atctacttca ccaatgacgc cgctaatatc aggcggaatg gaatagacgc
     5341 cgctaaaatg aacatcaccg aaaaataatc cacagcaaga cgtcaccaca atagaaacca
     5401 gcaccgcggc atgaaaatga cgtgatgaca acacggttat aataaaaaac cctaaaatac
     5461 ctaacaacac gccgtgagaa cttaaatcgc caatcatcac cagcgtgtct ttattggcga
     5521 caataacacc agtgtttttt aatcccatta aggcaataaa taatccgatt ccactggtga
     5581 taccaatacg taaacttaat ggaatgttgg agatcatcca gtaccggata cgaaacagcg
     5641 tgagcaaaaa tagcccaatt gcgccccaga atatcgcgcc catcccggtc tgccaggaga
     5701 tccccatcgc ccccacgacc acgaacgcaa agaaggcgtt cagccccatt gccggagcca
     5761 gcaccacggg taagttagca aatatcccca tcgcaatact gccgataccg gcaatcaaac
     5821 aggtggtaac aaacaccact ttcgggtcca tttgtgccgc gccgaggatt tgcgggttca
     5881 caaaaacaat gtacaccatg gttaaaaaag tggtcatccc cgcaatcaat tctgtgcgga
     5941 cggtggtccc atgctgaggt agtttaaata atcgcgaaag cgtccctgat tcattactca
     6001 cgtaatcggt attgtcatta ttcatttttt tgtccattgg aggagattta atcccttgct
     6061 tttgtaaaac aaatgacaac aacggttcag tgataattat cacatttcaa ttgcacatta
     6121 atagatattc tttaataatc tcgcgacgtt tctttatgat aaataataat caaattgata
     6181 aaatcaaaat gagaaaaata tgaataattc cattaaccat aaatttcatc acattagccg
     6241 ggctgaatac caggaattgt tagccgtttc ccgtggcgac gctgttgccg attatattat
     6301 tgataatgtc tctattctcg acctgatcaa tgccggagaa atttccggcc caattgtgat
     6361 taaaggacgt tacattgccg gtgttggcgc agaatacgct gatactccgg ctttgcagcg
     6421 gattgatgct cgcggcgcaa cggcggtgcc agggtttatt gatgctcacc tgcatattga
     6481 atccagcatg atgacgccgg tcacttttga aaccgctacc ctgccgcgcg gcctgacgac
     6541 cgttatttgc gaccctcatg aaatcgtcaa cgtgatggga gaagccggat tcgcctggtt
     6601 tgcccgctgt gccgaacagg caaggcaaaa ccagtactta caggtcagct cttgcgtgcc
     6661 cgctctggaa ggctgcgatg ttaacggtgc cagttttacc cttgaacaga tgctcgcctg
     6721 gcgggaccat ccgcaggtta ccggccttgc agaaatgatg gactaccctg gcgtaattaa
     6781 cgggcagaat gcgctgctcg ataaactcga tgcatttcgc tacctgacgc tggacggtca
     6841 ctgtccgggt ttgggtggca aagaacttaa cgcctatatt gctgcgggta ttgaaaactg
     6901 ccacgaaagt tatcagctgg aagaaggacg ccggaaatta caactcggca tgtcgttgat
     6961 gatccgcgaa gggtccgctg cccgcaatct caacgcactg gcaccgttga tcaacgaatt
     7021 taacagcccg caatgcatgc tctgtaccga tgaccgtaac ccgtgggaga tcgcccatga
     7081 agggcatatc gatgccttaa ttcgccgcct gatcgaacaa cacaatgtgc cgctgcatgt
     7141 ggcatatcgc gtcgccagct ggtcgacggc gcgccacttt ggtctgaatc acctcggctt
     7201 actggcaccc ggcaagcagg ccgatatcgt cctgttgagc gatgcgcgta aggtcacggt
     7261 gcagcaggta ctggtgaaag gcgaaccgat tgatgcgcaa accttacagg cggaagagtc
     7321 ggcgagactg gcgctgtctg ccccgccata tggcaatacc attgcccgcc agccagtttc
     7381 cgccagcgac tttgccctgc aattcacgcc cggaaaacgc tatcgggtca ttgacgtcat
     7441 ccataacgaa ttgattacgc actcccgctc cagcgtctac agcgaaaatg gttttgatcg
     7501 cgatgatgtg tgctttattg ccgtacttga gcgttacggg caacggctgg ctccggcctg
     7561 tggtttgctc ggcggctttg gcctgaatga aggcgcgctg gctgcgacag tcagccatga
     7621 cagccataat attgtggtga tcggtcgcag tgccgaagag atggcgctgg cggtcaatca
     7681 ggtgattcag gatggcggcg ggctgtgcgt ggtacgtaac ggccaagtac aaagtcatct
     7741 gccgttaccc attgccgggc tgatgagcac cgacacggcg cagtcgctgg cggaacaaat
     7801 tgacgccttg aaagccgccg cccgtgaatg cggtccgtta cccgatgagc cgtttattca
     7861 gatggcgttt ctttctctgc cagtgatccc cgcgctaaaa ctaaccagtc aggggctatt
     7921 tgatggcgag aagtttgcct tcactacgct ggaagtcacg gaataataaa aaaagcccgg
     7981 cgtcatgccg ggcaaaagtc accagttacg tttatgccac tgtcaactgc tgaatttttt
     8041 tctcgcggcg gattttgcgt tcttccatta ccgctactat cgccatcagg cagatacaac
     8101 caatcgcggc gatatccagc gcggcgaagg tgcctgccca gccggtaagg ccgaataccg
     8161 gcgtcccatc ggcaatcatt cccagaccta acttggcaaa gctgtcacca atcaggtaag
     8221 caaaggtgcc tttaatacca tcggcagcgc caatcgcttt tttaggtaca aagccaacag
     8281 cagccacacc aatcaacaat tgcgggccaa agaccaggaa acccaacgca aagagagaag
     8341 ccagatagat atattcgtta ctggcatgtt gatacacacc gagcgtggcg ataatcagcg
     8401 ccagcgcgat gcaggccacc aggccacggc gaccgttcgc caggtcagag agccagcccc
     8461 acagcagcgt accgaccagc gcaccagctt caaacagcgt aaagccctga atcgccaccg
     8521 ctttagagag tttcagttcc tggaacgcgt atacggttga ccactggtcg ataccaatac
     8581 gtaccacata gaggaaaatg ttggcgaagc acagcagcca gatcactttg tttttcagca
     8641 catactcaac aaagatctgc cacttggtca tatcggtaga ttctgtctct ttgtcctctt
     8701 cgctgacctc ctcgccgaac agttcttcag ctttgccgag gccataagat tccggggagt
     8761 cgctaccgta acgcaggccg ataaaaccga caatcagcgc gataatcgac gggaagataa
     8821 acatgccgat gacatggcca tcgaacaggt aatttgcccc gaacagcgcc acacctgctg
     8881 cgcctgcacc gccaaggttg tgagaaatat tccagaaacc gaggaatgtc ccgcgtttac
     8941 gacgcggcgt ccatttggtg atggtggagt aactgcacga accgccggta ctctggaaaa
     9001 agccgcttaa ggcgtagaag gcaatcatca ggaacaggct aaccgagccg ctgcccatac
     9061 tggcactgaa gcccagcata caaatggcag agaggataag catgaacggc aggaattgtt
     9121 tggtgttttt gccgtcggcg tagtatgaaa ccaacgtttt acccacgcca taagtgatgg
     9181 agaaacccag gccgatcatc cccagctgcg tcatgctcaa cccgtaggtc gaaatcatat
     9241 cgttctgcgc gatgttaaag ttcttgcgaa tcaggtacat cgtcaggtag ccgataaaga
     9301 ccaccaggta ggattgcatg aacggtttga accacatttt gcgccgcact tcgagcggaa
     9361 ggtccagggt cggcttgcga acctggttta ggaaagccag catgggttac tcctgaaatg
     9421 aatacctgcg tgaggcatgc attgtaaaaa tcagcagcaa atcaggcctg agatagcgtc
     9481 caagaaaaac ctggaaaatt tcttagtttt gccggatatg aagtgaaaag gtgagatgca
     9541 tcacgcttca cgcggtgtct gggcgttcaa aaagggcagt aacagcagtg cggaaatccc
     9601 ggcggcgata gagataacca caaaaaatcc gctccagtgc caggtatcga gcactttcgc
     9661 cagcggccaa ccagcaagcg acgcccccag ataagcaaac aagccgacaa accccgtcgc
     9721 cgcccctgcc gcctctttgt gggaacactc tgccgccgcc ataccaatta acatctgcgg
     9781 gccaaagaca aaaaaaccaa tggtgaagaa gcaggttgcc tgcatcacgt agctggcaaa
     9841 tggcatcagc cacagcgagc cgactgaaag caaaattccg gcggcgaaaa tcaaattcat
     9901 cggcccacgg ttgccgttaa acaatttgtc cgagccccaa ccggctacca gcgcaccgat
     9961 aaatccgccc agttcaaaca tcgtcactgc cgtattcgcc gtgaccagat cgacgcccag
    10021 cgtctcggac atatacaaat tgccccagtc gttgatcgcc gcccggacca catagaccag
    10081 cacatagcaa aacgaaagca gccagatata cggattcagc aacacatatt tggtgaggat
    10141 ctctttacgc gtcaaccctg ccccttcttg ttgttgagca atttccagcg cgtcgtgccg
    10201 ccattcaccg accgccggta aacctaacgc ctgcgggcga tcgcgtagcc gccagcagag
    10261 aaaaatcccc acgactatcg ccatacaacc agcaatcatc atcccggcac gccagccgta
    10321 atgcagcgcg ctcgctgcca tcacaatggg aatgagtgcg ccgccgacgt tatgcgccgt
    10381 gttccataat gcccaccaac cgccgcgctc ggtacgtgaa taccaggccg ttaacagacg
    10441 cgcacacacc ggtgaacccc agccctggaa aaaggcgttc agcacccaga gcacggcaaa
    10501 cgcccatagc gacgtcgaga agccaaacag aatgttcatg atgcccgtgg cgataagccc
    10561 tatccccata aaataacggg catttgagcg atcgctgaca atgccggaga caaacttcga
    10621 cacgccgtaa gtaatgtaaa acagggtcgc taacaggccg atatcgctac ggctgagcac
    10681 gccgttagca aggatttctg gtaccgcggc gttaaaactt ttccgcgtga agtaaaacag
    10741 cgcgtaaccc agccagatgg tcagcagaat atgccgacgc cagtagcgat aacgggcatc
    10801 aatttcatgt ttatcagtca ttaatggcgc atcggcaggc gctttcagaa acggcaacat
    10861 catcgaacct tagacataac gttgaggtag agaaacgctg acacgcgtgc cgtgcagaca
    10921 ggaaatgtgt aatgtgccgc ccagcgccgt tacgcgctcg cgcattccgg tgaggccaaa
    10981 accttgttgc ccggaacccg gcggcaaacc gctgccatcg tcttcaatca ccagcatcaa
    11041 ccgttcatcc tgctgccagc cttgcagcgt aaccgcgctg gcatcggcat gtttcacaat
    11101 gttgttcagc ccttcctggc agacacgaaa cagcgtcacg cgctggtttt cgcttaatgc
    11161 tgattcatcg atacgccatt cgagatggct gacaatgccg cgcccttcca gctccatttc
    11221 ccgcatcagt gagcggatgg cctgctccag ggtgagatca tccagctggc gcggacgtaa
    11281 ccgtcctaac aaacggcgca ccgcgtcgta aacgcccagc gacagttgtt cgatgagctg
    11341 cccgctctgc ttcacactgg cgttatctgc cgccagccgc tgaacaatgc ccgcctgagt
    11401 acgaatagca gtgatggtct gaccgatatc gtcgtgcagc tcacgcgcca cgtcgcggcg
    11461 cacgctctct tcggtttcta acaaacgttc agccagatgc tgattgcgcg ccagttcctt
    11521 ttgtagcgac tggttaagtt cacgcaaccg ctggatgcct gcgcccagta acaaccctgt
    11581 cagactttgc accagcaacg agagtaataa atccaccaga tgatcgcgcc aggtttgact
    11641 ggcgatcagc gcgatggcgt tcatcaacgt cgcaatcagc gctccttgcc agccatagtg
    11701 ccaggccagc gcgataatcg gcagcgccag acagaatggc gtaaagcgcg acagttcgtc
    11761 cggcaatccc aactggagcc agagactgat aacaaacagc agcaagtacc agaccagatg
    11821 ccgcccgcgc cagttgatgg gctgagaaac cagtgacggg ccgagcggca gccaggtgtt
    11881 attggcgaga tagtgccaga acaccagaca tatcggggcc agcgtcagac cgccagttaa
    11941 agtcagcaac agcgcattcc acgactcttt gccgtgccaa agccagggca gcgactgcaa
    12001 caacgccgcc gccgttaacg ccgcaccctg taacagcaag gtgcgccagt cacgctgatg
    12061 gcgatagcgc gagatcagcg ctacgggcag taacgtcagt aaactaccga tcatcaataa
    12121 cggaaaatgg gttaaaccga ccgcctgcgt tagccagtaa atcagcagcc actccgcacc
    12181 cagcaatacg ggccagtatc cgcgcgggca ttgcagcatt agccccagac gcagaccaaa
    12241 cggaaataac agcaccgcca tatcagggcg ctcaaccaga tgcaggctga tactccatag
    12301 gcaaaaccat gcggcagaga agataaaaaa gcaggcaata acggtaatta agcgggagaa
    12361 caacgtcttc atcaccagcc atcaaacatg cggcgcgcca gctctacgtc gttactgacg
    12421 cccagttttt ccatcagatt ggcgcgatgg acgtgtaccg ttttcggtga caagcccagt
    12481 tcggcggcaa tctctttcac cgccattcct tgcgccagtt tttccgccac ctggcgttca
    12541 cgtttggtca gcgggtcctg acgaccggat gccagtttaa tggcaatatc cggcgtcaga
    12601 taacagccgc ccgtggcaac cgtatgcacc gcagcaatga gttcatccgg gctacagcgt
    12661 ttggaaagaa agccgcgtgc ccccgcgtta agcgcctgct caaccagcgc aggactgtcg
    12721 tgaacggaga gcataatcgt cgccatacct ttcggcagct ggcttagcag ctccagaccg
    12781 gagatatcgg gcatggagat atcgcaaata cacacctgca caccgcgccc cggcagcccc
    12841 gccagcgcct cgcgccccga accaaactcg gcaactacct gcaaatcagg ttccagcccc
    12901 agcagctgcg caaagccgga gcggacgatg aggtgatcgt ctataagggc aacggtgatc
    12961 atggtcttgt cctggcgggt aaaaaaaaac gcgcttacct taacgataag cgcgatgttg
    13021 ttcaagcctt gagcggttac tgaaaaaaca ccgcgatctt gttaaacatc gtcggatcgg
    13081 actgattacg ctgcactttc acgacatctt ccagcttatc gatttggctt atcatctgct
    13141 ccagacgctg gtcgtcattg accagtagcc agatatggct tttgtcgctg tcctgaatcg
    13201 gcagacaaag aatgccttca acgttaaaag cgcggcgggc aaaaaggcca caaacgtggg
    13261 tcattacgcc cggatggttg cgaacggtga gctccagaat tacgttgtta tgagttgtgt
    13321 tttgcatggc ttattccccc accatttcag tattcgccgc acctagcggc accatcgggt
    13381 aaactttttc ttcggcatca atgcgcacat ggatcagcgc cgggccaggg cgattgatta
    13441 tttcctgcaa tgcagcctgc ggatcggctt cgttattcaa atcacaggtt tcgaggccga
    13501 atccggcggc aatctgcata aagttgattt tgcccggata ggtggcggca aaaacgcctt
    13561 gctcgtagaa cagactctgt tgctgatgca ccagccccag cgcttcgttg ttcatcagaa
    13621 tgattttgac atccagctga ttttcactgg cggtcgccat ctcctgaata ttcatcatca
    13681 ggctgccgtc gccggagaaa cacaacactt tgcgatccgg gttcgccagc gccgcgccaa
    13741 tcgccacagg caggccaaaa cccatcgtgc ccagcccacc ggaggtcagc cactggcgtg
    13801 ggcgattgag cggataagct tgcgcggtcc acatctgatg ctgacccaca tcggtggtga
    13861 taatcgcatt gtcatcgaca caggcggcaa cggcgttgat caggccgtaa tggcttaatg
    13921 gatcgcacgc tttcgggatt ggacacggga attcgcgctg caaatccgct accaactggt
    13981 gccactctgc acgcagttgc gcttccacct gcgggatcaa ttgcgccagc acgtcatcaa
    14041 catccgcctg aatcgccaca tgcggctgct tgattttacc cagctctgcg cgatcgatat
    14101 cgacatgaat aattttggca ttcggacaga actgctcggt tttgccaatc gcccggtcat
    14161 caaaacgcgc accgagcaca atcaacaaat ccgcctcctg caaaatatag ttggtactgc
    14221 gcacgccgtg catccccagc atacccagcg acaacggatg cgcttttggc agcatgccca
    14281 gcgccattaa agtcatggtg gtaggcagtt gcgctttctc cgccagttca cgcacccgcg
    14341 caggcgcatt gatcacacca ccacccagat aaagcaccgg gcgtttggca gcgttaatca
    14401 ttgtagctgc gtcacgaatg ctttcttcgc taaaggaggg tgcagcggct ttttctgcca
    14461 ccgcgggctg agcttcaatc tcaaaaaccg ccgtttgtac atccttagga atgtctatcc
    14521 acaccgggct agggcggcct gattgcgcaa tgcggaaggc atcgctcatg acctgtggga
    14581 gctcttcgat atgtctgacc agatagttgt gtttggtgat ggggatagag atgccgtagg
    14641 tgtccacttc ctggaaggcg tcggtgccga tcatcgaggc gggaacctga ccagtgatgc
    14701 aaatcagcgg gatggagtcc agccgcgcat cagcaatggc ggtcaccagg ttagtcgcac
    14761 ccggcccgct acaggccata cagaccgccg gtttaccgtc ggtgcgcgcc attccctgag
    14821 cgataaaccc cgcgccctgt tcatggcgag ccagaatatg gcggatttgc gtgctttggc
    14881 ttaaggcgtc gtaaacaggc aggatagaac cgcccggaat gcccgtcaca atcttaatgc
    14941 cctgctgttc caggaaatga acgataaatt ctgcgccggt aaagcgctta cgcgtcgatg
    15001 ttgtgcccga acttgccatg ctccagtcct tttcttctgg gccgactttc cgggaggtgc
    15061 ttaaacgaaa aaccccgccc ggtttgcgcc ggcggggttt tggaatcgtg tgttgttcca
    15121 gtccctacgg cgcattgccg acgaccacca ccacacgcac gacgaccact gcggcggatg
    15181 gcgcagttgg tagtagtttt gcgttgaaca tggaagtagt cattggggac cttgttggtt
    15241 ttgtgtttaa caatatttat acaagcacag ctttacaggg gagacaatgg aaaatttttc
    15301 agcaagggaa aattgagggg ttgatcacgt tttgtactga attgcagata acaaaaaacc
    15361 ccgccggagc gaggtttcgt cagccgcctg cggctggtaa ccgcaaagca cactgtatta
    15421 tgtcaacact gaaagtatac gtgttccgcg cagaacgcgc aatttcggca cgaattttga
    15481 cgtatttagt gcatagttga gtatcgttca cagtttgcgt tttgtccaaa tattactgtt
    15541 tatttataca gtaaacttct ataatatcac tgttcgcaat gtgttatgcg ggggccgcat
    15601 cgttacccgg cgcactaagt cctggctgaa acgggtggtg ccgtcagcgc cttaaccccg
    15661 cgtgagcaca ctgtgttatg tcaacaagca caacgtttct ccttgagata ccgcgtgcac
    15721 aacagctggc aacaggcagc ggaaaggtac gtcagctggc agtgctcctg aaccacagga
    15781 gacgcgtatg agcctggtgg atatcgccat tcttatcctc aaactcattg ttgcagcact
    15841 gcaactgctt gatgctgttc tgaaatacct gaagtaattc agattcaagt cgcaccaaag
    15901 gggagcgggt aaccgctccc cttttatatt tagcgtgcgg gttggagtcg gatgcgatgc
    15961 tgacgcatct tatccgccct accatccctc ccggcaacat ttattgccgc ttttgtttac
    16021 atattctgcc gctaaacaat tccccattcc tggcgtatat ctggctaaca ttcatcaatg
    16081 tgatagattc ctctcccgca tttatgggaa tgcgtagtga cttattctaa ttatttttat
    16141 aaaagcatcc gtgataatga aaaggcaaag aaacgtcaat ttgttattga tgttggtatt
    16201 actcgtggcc gtcggtcaga tggcgcaaac catttatatt ccagctattg ccgatatggc
    16261 gcgcgatctc aacgtccgtg aaggggcggt gcagagcgta atgggcgctt atctgctgac
    16321 ttacggtgtc tcacagctgt tttatggccc gatatccgac cgtgtcggtc gccgaccagt
    16381 gatcctcgtc ggaatgtcca tttttatgct ggcaacgctg gtcgcggtca cgacctccag
    16441 tttgacggta ttgattgccg ccagcgcgat gcaggggatg ggcaccggcg ttggcggcgt
    16501 aatggcgcgt actttgccgc gtgatttata taaacggaca cagttgcgcc atgctaacag
    16561 cctgttaaac atggggattc tcgtcagtcc gttgctcgca ccgctaatcg gcggtctgct
    16621 ggatacgatg tggaactggc gcgcctgtta tctctttttg ttggttcttt gtgctggtgt
    16681 gaccttcagt atggcccgct ggatgccgga aacgcgtccg gtcgatgcac cgcgcacgcg
    16741 cctgcttacc agttataaaa cgcttttcgg taacagcggt tttaactgtt atttgctgat
    16801 gctgattggc ggtctggccg ggattgctgc ctttgaagcc tgctccggcg tgctgatggg
    16861 cgcggtgtta gggctgagca gtatgacggt cagtattttg tttattctgc cgattccggc
    16921 ggcatttttt ggggcatggt ttgccgggcg tcctaataaa cgcttctcaa cgttgatgtg
    16981 gcagtcggtt atctgctgcc tgctggctgg cttgctgatg tggatccccg actggtttgg
    17041 cgtgatgaat gtctggacgc tgctcgttcc cgccgcgctg ttctttttcg gtgccgggat
    17101 gctgtttccg ctggcgacca gcggcgcgat ggagccgttc cccttcctgg cgggcacggc
    17161 tggtgcgctg gtcggcggtc tgcaaaacat tggttccggc gtgctggcgt cgctctctgc
    17221 gatgttgccg caaaccggtc agggcagcct ggggttgttg atgaccttaa tgggattgtt
    17281 gatcgtgctg tgctggctgc cgctggcgac gcggatgtcg catcaggggc agcccgttta
    17341 agcgcacgtc accgcagcat cgtcatcagc tccatgggag aacgatgctg ctttatcaga
    17401 tcacgcatca cccacatatg cggtgcggag taagaataaa acgcctgata gcccgcacaa
    17461 agcacgctgt ctggcgtctc ctgccgagcg tgcgggcaca ggcgatgcca gtcgcacgcc
    17521 tggcagtcac ggcgcggttg cgggttttcg ctgccacacc atttttgtaa ggtggcgtca
    17581 aaaagctcaa tcgagatacg actgatatct gcccgcaccc aacactcaaa cagggcaatt
    17641 aaaaatttcc cccacagctc tgctgtcacc gaagcggccg tcagatcgcc ccggtcatca
    17701 aattcaagat ggggaatgag ctgtacggag cggatgcccc gctgtttgaa attgtgataa
    17761 aaatcggcag gttgcagagc atccttgtac tctacaacca gcttatgcct gtcctcttca
    17821 gcatcaatga cctgacttcc tgtcatgcga ctctctcaat aaatacgata agttgatgta
    17881 tatgcgttac agcaaacagt attgcgagag ataacaccgc gagggagatc aaaaatttgt
    17941 cacggactac gtgaaattgg gaaaaatcgc tattcgtgac atccattaaa ttgcgattac
    18001 gggtgtagtg ccagaggatc agcgccacga tggcgaggat gccaatcgaa atccagaata
    18061 acatgcccgc ctggtgccag ttgtgtttga tagccaacgc catcaacgcg ccgtagccca
    18121 gcatggtacg aaaccacgcc agtgatgtgc gctccggctg cagccctggg tcggcaatgc
    18181 gtctggcttt acggctatcc ggcatacaac accagtccca taacgatcac cgccacaacc
    18241 atcaatatta agctgatgat taataagctg ttggtgtagg gcaggtcctc tttcagacgc
    18301 atcgcttttt cattgcgtaa ccagcgcagg tagccataca ttgctaaccc gccggaaaac
    18361 agacacaaca acaacgccag cagttcacga atgacgggcg tggcgaaatc tggcgcaagt
    18421 tgatcaagcc caacccccgc cgccagaaat cctagtgcgg tacggatcca cgccagaaaa
    18481 gtacgctcat tagccagcga gaagcggtaa tcgggcgctt ctccaaggcg ggaaattttc
    18541 attgttgctc ctttgcaatt tcactaacgc tctatcttta gcggtttttt ttaagaattt
    18601 cgtccgcccg tttcctgata tgcttttgcc atccgctaca aggaggtcag atgggtatca
    18661 tagcgcaaaa taaaatcagt tcattgggca tgctgttcgg tgccatcgct ttaatgatgg
    18721 ggattatcca cttttcattc ggcccttttt ccgcaccacc gccaacgctt gaaagcatcg
    18781 tggcggacaa aacggctgaa attaaaagag gtcttctcgc cggaataaaa ggcgaaaaaa
    18841 ttaccactgt ggagaaaaaa gaggacatgg atatcgataa aatcctcgat caaagcggta
    18901 tcgcactggc cattgctgcg ctactttgcg catttattgg cggtatgcgt aaggaaaatc
    18961 gctggggcat tcgcggcgcg ctggtttttg gtatcggcat cgtgtgcagc attttgctga
    19021 tattcctgat tttttccttc ctgaccggcg gttcgctggt ttaagcagcc tgaataatgc
    19081 ccgccgggtg atcatcagcg ggcattatcg tcagaatttc tgttttttct cctccacctt
    19141 cactccttgc gtcggtaggc cggtgtcata gtcgcgtacc actggcgaat agccatcttg
    19201 tgggcgtggg cgaaacgccc ccatccagcg cggtcgtgca tctttacgcc acggacgcag
    19261 actccattgg taactgcgga acggatcgcg aattttgtcc atgtaatcca ataaggcgtc
    19321 atgcattttg ctgcgaacgt ctgcaaaacg gatatcatcg atcaggttat gcatttcatt
    19381 tgggtcatta cggcgatcgt aaagttcatc actggtgaag aggttgagca ccagtttaaa
    19441 gtcatccgtc acccagcaac gcaccggaat aaaaccgcca aagctgtcat gctcaatctc
    19501 gtagcggtta aattccacca tcacgccgcg tggctctttc acggcaagga tattttcccc
    19561 cggcagaatc tctggttttt caatatctgc cagcgccatc attgtcggca gtaaatcaat
    19621 atgactgact ggcgtatcga cctgtcgccg ctccccttgc ggcgaacgga tgatcagcgg
    19681 aatacgggtg atgtcgtcat acatcgccgc tcctttactg atcagcttat gtgcgcccat
    19741 catttcaccg tgatcggagg tataaatgac ccacgtattt tcacgttgct ctggcgttaa
    19801 ggcattgatg acacgtccga tttggtcatc aacaaagtca ttgcaggcaa aatagagcgg
    19861 atggtgataa agcccgtcat caccgactgg cgatagcatc gcctgcgccc ataagcggtg
    19921 atgttccggt ttgttcgcca ggtcatcctg tgatttctcg ccaagttcgt agtaaaaatc
    19981 agcgtatttc tctaaatact ccaccggaca ggtgaacggg tgattcggct catcatacga
    20041 aaccaccatc aggaagggtt cctcggcgcg cacgggctgt tgcagaaaat ctaccgctcg
    20101 attgctgatg cggtgcgccc aggtgaaggt ttcgtcaata tggttcgcct gtaaatcttc
    20161 gacgctgttt aggccattgc gccacaggct gatctctttt tccgtcagtt cgctaagata
    20221 gttcgcccca tcaaaccagt aatcagcatc ccactccggc ggacactcgc cagtgccgaa
    20281 atagtcatga ccgtcgagat gccatttgcc gatgtaacag gtgtgatagc cggcatcctt
    20341 aaagtagcgc cccatagtgg agatgttttt gcctggcgcg acgttgttgg tccacgggcc
    20401 ggactggtta gcgtagatac cggtgaatag tccggcgcgt gcaggcgtac aaaccggtga
    20461 acaagtgtag gcggaattaa agcgaatacc ttccgccgcc agactatcaa tattttgcgt
    20521 attcagcggt ttaccgctat agcaaccgac catattggtg gcctgggtat cggtcaattg
    20581 gggcgtttca taggctatcc ttcgcgtcgg gagtaaaggt tacggctggg tcgtgacgat
    20641 gccgccagct gtcaaaaatc aggtagacaa tcactacggc agcgatgaaa taactgatca
    20701 tcgctaacca gcgcgtaccg tagccgccga attcagccaa cccgacatag acgccaatca
    20761 tcgcgaacag gataccaatt gacgcgatct tgacgttttt ccacggtttc atgtccaccg
    20821 caaacgcatc tttgaaggtg aacggcgtgg cgcgcgcttt gataaaaccg atcaccagca
    20881 tcacgaccac gttgatgcag aacgtacagg ccagcacata gaggaaatgg aagtcgaact
    20941 tcaccagata gttgatggtg atgtagctga ttatgccaat ccccatcgcc acttttgccg
    21001 ccagcgccgg gatgcgcggg aagaaaaagc ccataatgat gatggtaacc agcggcacgt
    21061 tgtaaatgcc gttgagctgt ttcatccagc tatacagccc ctgcggcgcg ttggcgatcc
    21121 acggcgctac caaaaccgaa acgatagcga taaagaaacc aaatttgcgc ccgacggtga
    21181 ccagctgctg cggctcggca ttctggttaa tgatgcgacg gtaaataccc atactgaata
    21241 atgtactggc gctattcaga aagccgttga aggtactgat caccgcacca aataacaccg
    21301 cgccgaagaa acccaccagt ggcactggca gaacgttatt gaccagcgtc gggtaggcca
    21361 tgtctgcttt cggtagatcc tgatacagat gaaacgcaat caaccctggc agcaccagta
    21421 ccagcgggtc gagcattttc agcaccgccg ttaacagcgc ccccttttgc ccttccgcca
    21481 ggctttttga cgccagcgtg cgttgcacga tgccctgatt tgtacaccag taaaaggtgt
    21541 tcaccagaat taaaccggta aatgccgcgc caatcggcaa gggatcggtc gggccaccga
    21601 ctgagtttaa tttctcggcg tgaacggtgg taatttgctc aatgccctgc ataaagctgc
    21661 ccttgcccat cgcgatcagg ccaaataccg gcaccatcaa cccgccgata accagcccaa
    21721 taccgttgat ggagtccgcc actgccattg cgcgcagtcc gccgatcacc gcatacaaaa
    21781 ttcccgccag accaagcaaa attaccagta gccagatagc cgcaccgtga gaaatctgta
    21841 gcgattcccc gacgtgaaac aggctgttga gcgccaacgc gccggagtag agaacaatcg
    21901 gcagaaagca gacgccggtg gcaatgagga agcagaagtc gatgataata cgcgtcgttt
    21961 tatcataacg ttcctccaga aaatcgggga tggtggcaat gccgcgcttc agatagcgcg
    22021 gtaaaaagat tagcgcgagg aagatcagcg tcaccgctga agtcacttcc cagcccatca
    22081 ccgacatgcc gcttttgtag gcctgcccgg aaagaccaac cagttgttcc gtggaaaggt
    22141 tggttaacat taacgaagcg gcaataaccg gcgcttttag tgaacgtccg gcaagaaaat
    22201 agccttgttg cgatccggta tctgttttgc gaaccttcca ctaggtgatc accgccacca
    22261 gcagcgtaaa accgacaaaa ctcaagattt gtaacgaatt catctcatag cccttattta
    22321 tgtttatgtc ccggacgcat gaatataggg agaggtaaaa tgtgtcgggt caggcagtca
    22381 gattcttaaa atacaaacgt cgtatccctg aacggattgt tttctgttaa gttcaggttg
    22441 tgagcataat tcgccggagg gatattaaaa tgaatggaaa attgcaaagt tcagatgtaa
    22501 aaaacgaaac tccgtacaat attcccttat tgattaacga aaatgtgatc tccagcggaa
    22561 tttctctgat ctcgctctgg catacctacg ccgacgagca ttaccgggtg atctggccgc
    22621 gggacaagaa gaaaccgctt atcgccaact catgggttgc ggtgtatacc gtacaaggat
    22681 gtgggaaaat tcttttaaag aatggcgaac aaataactct gcatggcaac tgtattatat
    22741 ttttaaagcc aatggatatt cactcttatc actgtgaagg tttagtcttg gaacagtact
    22801 ggatggaatt tacccccacc agtatgatgg atattcccgt tggtcagcaa agcgttattt
    22861 ataatggcga aatttataat caggaactca ccgaagttgc tgagttaata acttcaccag
    22921 aagcaataaa aaataatctg gcagtcgctt ttctgacgaa aattatttat cagtggattt
    22981 gtcttatgtc cgcagatggt aaaaaagatc cacaacggcg gcaaattgaa aaattaattg
    23041 ccactttaca tgccagtctg caacaacgct ggagcgtagc tgatatggct gccacgatcc
    23101 cctgtagcga agcctggttg cgtcgtctgt ttttacgcta taccggcaag acgccgaaag
    23161 aatattacct cgatgcgcgt ctggatctgg cgctatcgct attaaagcaa caaggaaact
    23221 cggttggcga agtcgctgat acgctcaact tcttcgactc ctttcatttc agcaaagcct
    23281 ttaaacataa atttggttat gcgccgtcag ccgtgctaaa gaatacggac cagcacccaa
    23341 cggatgccag tccacacaat taacgtaact ctggccagta cgctttgttg gcctccagca
    23401 actcatccag aatagctttg gcgaccgagg cgctcggtac cgttttcgac aacgtaatcg
    23461 cctgccacag gtgctgatat gaacgctgtt cccaggcgtc cacgaccagt ttttccaccg
    23521 ccacttgctg actcattaac cctttctgaa actgcgggat atcgccgacc accagtggtt
    23581 caggccgtag tagcgcacgc gcatctgttt gcgatcttgc agcccaacaa tttgcgccat
    23641 ccggctttca ataccgattg gcatgtcaca gatgttgagg attttcgcat tcgggcgcag
    23701 acgacgcgtg gcttctgcga caattgctgc cgggttggag tagttgagca tctaggcgtt
    23761 tggtgaatat ttttccatat aatccaccag ttccaggacg ccgccaatgg aacgcatgcc
    23821 gtacgctatt ccgccgggtc cgcaagtttc ctgaccaaca acaccgtggc gcagtgggat
    23881 tttttcatcc agttcgcgca tcgggtactt gccgacgcgg atgtgcgcca tgacaaaatc
    23941 aacgtcgctg aacgccactt caggatcggt ggtgtaacta aacgcaatgt ccggcgcttt
    24001 ttctttaagg atgactttac aggcttcggc aatcacttcc tgccgcgcgc catcgttatc
    24061 ataaaatttc agtgcacgaa gcgggaaacg gtcctgattc gccaggagca tcaacacgat
    24121 gcctggcgta aaggtgcttc caccgcctgc gacaaccact gagaatttgg tcattatact
    24181 gcctccgtaa tggcaacatt ttctgctgat tgatgagaat taattaagct atcgagctgt
    24241 tcacgcaatt gggatacatg cagaccaatg atcacctgaa tggcatcgcc actacggaag
    24301 acgccgtgcg ctcccagctt tttaaagact tcgtcatcca gcgtttgtga catgtcatgc
    24361 agtgcaatac gtagacgcgt cgcgcaattg ttaatgctgg agatattgcc gaccccgccc
    24421 agggcttgca ggataccggc agcctgatcc agctcttttt ttggctctgc agcggtggtt
    24481 tggcctcgcg aggctttgta ttcggctttt gagtagagtt tcacttccgc atcttcacgt
    24541 cccggcgtgc acatgttgaa ctgcagaatc agggtgcgga aaaccacgaa gtacagcagg
    24601 gtaaagcaca acccaatggc gatttgggtc agcatcatat ccgcgtggtt gctgaacatc
    24661 gggatccagt tttgcggtaa aacctggtca atcagacctc cgcccatgtt gcccaccaca
    24721 ccaaagagat acattacggt cgacattgag gccgccagca cggcgtgtac cgcaaacagc
    24781 aacggtgaaa tgaacaggaa ggtaaattcc agcggttcgg taattcccac cagcatggcg
    24841 gttaaggttg cgggaatcag caagcccgcc acttttaccc gattttccgg tgcggcagtg
    24901 aagtacatcg ctaaagaaat gcccacggca ccaaagattt ttgagttacc gtgcagggca
    24961 aaaccgcctt ccgggaacaa cgatttcagc ggctcggcgc tcagactgaa ctcttgcaga
    25021 tgctgcgccc agtacatctg aatgccgcct tcaacagctg ccggaccaaa gataaacggt
    25081 ccgtagatga agtggtgtaa accggttggg atcagaatac gttcgaggaa ggtgtaaacc
    25141 cacaccccaa gcgcacccgc cgaacgcagg aacgcttgca gagattcaat ccccatttgt
    25201 acttttggcc agccgagcaa tgtcagccag gcacagggga tcatcaccaa gaaggcgata
    25261 atcaccacat aagacgtccc ctggaaaatg ccgagaaaaa ccggcagttt tttatcgaac
    25321 agacggttat gcagcgccgt cacaatgccg gaaatgataa ttgcgccgat aatgctggta
    25381 tcgagggttt taatcccggc catcattgtc agaccactac ctgccaccgc gtcctgagtg
    25441 aaatcgacgc cgaagtagct tccccaggtc attcccatcg cattgatgaa atagttccag
    25501 gtcaggaaac tcaccatcac cgccagacaa gcacgtccct gcgcttgctt agcaaggcca
    25561 atgggtaaac cgacagcaaa aatcagcggc atattacgga ataccgtcca accgccctct
    25621 tcaataatgt gtacgatttg cgcgaataaa ctgttcggat cggtcagtga ttccccgaca
    25681 aacatcgggt tttgcagcaa gatggcaaga cccaccacaa tcccggcgaa gggaaacagc
    25741 agcactggcg tgaacatcgc gccgccaaag cgttgaattt gactgagcat ttccacatcc
    25801 tcttttctca attctgtaag taggaagcct gattggttat gcggcagacc tggagcgagg
    25861 ataggaagat gcgatcggca gaacatgacg acccaatggg attcgtgatc gctttcatgc
    25921 ttttcgttgg gcggtagaga tctacgtttt cgcgaaaaag tagacatctg tgagcacagg
    25981 tacacagcat cgaacacatc tttaaaaaaa agattttttt caatcgatta agcagaactt
    26041 gtgggcgcat tacccgtgct tgcaggcaaa aaagagagct agagatgatc tacaaaagca
    26101 ttgcggagcg gttaagaatt cgacttaatt ccgcggattt cacgctaaac ggccttcttc
    26161 ccggtgaaaa aaagctggcg gaagagtttg cggtatcgcg gatgactatc cgtaaagcca
    26221 ttgacctgct ggtagcgtgg gggctggtgg tccgccgcca cggcagcggc acttacctgg
    26281 tgcgcaaaga tgtgctgcat caaaccgcca gcctgaccgg actggtggag gtgttaaaac
    26341 ggcagggaaa aacggtcacc agccaggtgc tgatttttga aatcatgcct gcgcctccgg
    26401 ccattgccag ccagttacgg attcaaatca acgagcagat ctacttctcc cgtcgcgttc
    26461 gttttgtgga agggaaaccg ctaatgctgg aagacagcta tatgccggta aaactgttcc
    26521 gtaacctttc actgcaacat ctggaagggt cgaagtttga atatattgaa caagagtgcg
    26581 ggattttgat tggcggtaat tatgaaagcc tgatgccggt gctcgccgat agactgctgg
    26641 cgcggcaaat gaaggtagcg gaacacacgc cactgctgcg gatcacctcg ttgtcatata
    26701 gcgagagcgg ggagtttttg aattattcag tgatgttcag aaatgccagc gaatactagg
    26761 tggagtacca tttacggcgc ccccacccgg aaaagagtta accgatactc cagaagagca
    26821 ccgccagtaa ttggggggtg ataatgcgca ggaacatcac taacggatag acagtggcgt
    26881 aagagagcgc cgccgcacca ctggttggat gaagattatt agcaaacgcc agcgccggag
    26941 gatcggtcat ggaacctgcc agcatcccgc acatggtcag gtaattcatt ttggctaaca
    27001 tccgcgccag aatgccaaca gtaatcagcg gaacggcggt gatcagggca ccataaccaa
    27061 tccagcttag cccttcgcca ttgaccaggg tattcacaaa atccccacca gatttcagac
    27121 caacgaccga gaggaacagc acgatcccca gctcccgcag cgcgaggttg gcacttggcg
    27181 gcataaacca gtacagcttg ccgatactgc cgatacgccc gaggatcaac gccataatca
    27241 gcggaccgcc cgccagcccc agtttcaacg cggccgggaa tcctggcaca aagacgggaa
    27301 tagaacctaa caatacgcca agcccgatgc caataaacac tggcagcatc tgaacctgtt
    27361 gcagtttttg ctgcgcattc cccagcacat tggcaacggc atcaattgcg gacggacgcc
    27421 ccaccaggtt gaggatatcg ccgaactgca ggctgatatc gccactggcg accagttcga
    27481 ccccggcacg gttcaggcgc gagataacaa cgtcatagcg ttctttaaag tgcaggtcgc
    27541 gaatacgttt tccgagcacg ttttcattgg tgaccaccac acgctcgacg cgcaaatcag
    27601 tgcctttcgt agacagcgag gtatcgacct cctgaccaat caccagttgc gcattatgta
    27661 aatccgctgg ctgccccacc agatgcagca aatcgcccag ttggataatg gtatctggcg
    27721 aaggtacttt tagggtttct tctcgtttca gacgcgagca gataattttg tcgccgttga
    27781 gaatcggtac atctttaatg gctaaatcat gcaggttagg gttctcaacg cgaatattga
    27841 tagtcttgat cagcgcgccg ccattggtgc gtgaagactc gtgctgctga gcttctgtct
    27901 cgacattgac gcggaaaata acccgcaaca tccacatggt gaacaaaatc ccgcaaatgc
    27961 cgaatggata cgccatcgcg taactcatcc ccatctgatc gaccatttcc attggtgtac
    28021 ccaggtcgcg caagatctgc tgccctgccc ccagcgctgg cgtattggtt accgcaccgg
    28081 agaaaatccc cagcactacc ggcagtggaa tatcaaacag tttatgcagg atggcggtaa
    28141 ccagaccacc gatgatgacg atcagaacag caaacaggtt gaggcgtaat ccggagacgc
    28201 gcaatgaggc aaagaagccc ggccctacct gaatcccgat ggtataaacg aacaggatca
    28261 ggccaaattc ctgaataaca tgcagcatat cgctacttaa tgtcatcccc gcctgagaaa
    28321 caaaatggcc gacgatgatc ccaccaaaca gcacgccgcc aatacctaat cctatgccgc
    28381 gaaatttgac gttgccgata aacaaaccga cgactgccac caaagccaga atactgaccg
    28441 ttaatgctat atcactcatc gtccatttct cttgagataa cattttaatt gctacggatt
    28501 atggctgaat cggtgaagca gatttgcctt atagcgcaca ttatcacggg aaagaacgtg
    28561 ccgaaatatc ttaaacagtc gaaatttaac gcattgttga agaagaaaga caaaaaaagc
    28621 cccaccagaa tggcggggca aagagaatag ctagttagct atttaacgcg ggacgttcgc
    28681 tgatagcgat acgctgcgct gcgatgggtt caggctcatt acgaattaaa tcaatatgca
    28741 gtaaaccgtt tacgaaggtt gcgccagaga cttccatatt ttcagccagc gtaaaactca
    28801 ggctaaatgg ctgattcata agcccttgat gcagccattt tttctcttct tttggctgct
    28861 ccggcgtgcc ttttacgctc aggcgcgtac cttccagttg aatctctaaa tcttcctgac
    28921 ggaaacctgc cagcgcaagg gtaatgcggt agtggttatc gtcgcttttc tcaatgttgt
    28981 acggcgggaa gctctggctt tcaccggcgt tttgcagtgc gttggccagt ttgtcaaaac
    29041 cgatccattg acgcatcagt ggggataaat cgaagttacg catagtcatt tctccttcta
    29101 agaagcgagt aagtacctgc aaatccgaag attcgcatat gctccctgac ggcgagcatg
    29161 gagatgtcag gccgcgccag gcggccttag ggaattagtt gatttcgata cggcgcggtt
    29221 ttttcgcttc cggaatcacg cgttcgagat cgatatacag caaaccattt accaggttag
    29281 ccccacgaac atgaatgttc tcagctaact ggaatttgcg ttcaaagttg cgttcagcga
    29341 tgccctggta cagataggtg cgctcttttt gttcgtcggc gtgagcacct ttcaccacca
    29401 gcagattatc ctgggcggta atttccagtt cgctctcagc aaaaccagcc acagcgatag
    29461 caatgcggta atggttttcg tctaccagtt caacgttata cggagggtag ccgccattac
    29521 tctggctctg gttgttttct aagtggttaa acaaacggtc aaatccaata gcagaacggt
    29581 aaagcgggga taaatcaaag ttacgcataa tcaatagctc ctgaaatcag cgagaatgta
    29641 agaccttcca caatggacag gtcaggtagc cagaacaccc atcaggcgcg tcctcatcga
    29701 ctacgatgta aaatgggtct ggaaatgaac ttttcaagcc ttatcagcgg actttttttt
    29761 gcagtttatg gtctattgca tagactgagg gggcagcacg aaacgttaaa gtgcgatagc
    29821 cgccacagtg cgacgtaatg atgatgttat tttcagcaag ggatcgctat aactcatcag
    29881 gccaacaccc acgatcaaca gatgaattta ttatgataag aaatgtgttg ttagcgttca
    29941 tgatatgcag cggaatgaca ttactcggag gatgctccag cgtgatgtcc cataccgggg
    30001 ggaaagaagg aacgtatccc ggcacccgcg ccagcgccac catgattggc gacgatgaaa
    30061 ccaactgggg cactaagtcg ctggcgattc tcgatatgcc gtttaccgct gtgatggaca
    30121 cgcttttact gccgtgggat gtgttccgca aagacagctc ggtgcgctcg cgcgttgaaa
    30181 aaagcgaagc caacgcgcaa gccaccaatg cggttattcc accggccaga atgcctgata
    30241 actgatttag cgatcggttt cggtgatcca cagctggcca ccttccatcc acgccagcca
    30301 ttgaccatcc ggcgagaaga ccacggcgtc cgcagaaggt ggattggcgt ggttttcggt
    30361 taaatactca acctcgccgc tttgcgcatg ggcacaaaca attcgattat ccagcacaaa
    30421 gcccaaccat tctcctgacg gatgccagtt aaatgcagac tgaatatccg ttttgttatg
    30481 ggttaacttg cgcggttcgc cgccctgtgg cgagataagc cacagttgca caatgccgtt
    30541 atcatcacgc attaaaaacg cgatttgcgt accctgcgga ttacagcgca cccagtggcg
    30601 cgggtagggt tgtttccgtt ccacttaacg gcgcatcacc tgccgatttc cagctagctt
    30661 catcttgcgg taactcaacg ataaatagct ccggcacttt ctcgcccttt ggcgaaagtg
    30721 tgtcgccaat aaacgccagc gcgtgatttc ctacccatcc ttcttcataa gcacgattga
    30781 tttcatcact gccaggctgc ggcgtgggcg tggttttact caccagcaca caccagtggc
    30841 taccgctgta ttcacgcgga tgttgctttt gtacgttgac cgggccaaac ggtgcagcaa
    30901 ccccgacgtt tcgcaaatcc agcgccggat agagttgatg cattacatgg tcgttatagg
    30961 taaagctccc cctttcaccg ttcgggctaa agacatgcac atggctgcca ccgcgcagcg
    31021 ctcctggcgt atacggcgcg gtaatatcca ttgcatcgag attgctcacc ttgccgcctt
    31081 cagcaatgac tccgcgtcga tgatggaagt cgtaatgcca tgtttcatca ggattttcag
    31141 ggccgtgaat aaagacatat ttctctgact tcggatgaac ggtcaccacg ccgacgtgtg
    31201 cgccctgtga cgcgcgatag ataacctcga cctcgcctgt atggatattc acacgctcaa
    31261 tggtctcgcc ggtaaacgac gccccagaag gacgcacgtc aaataccagc cactggctgt
    31321 cgggcgtcca ggtattggta ttggtgagca ggtgattacg gggagcaaag gttatctgtt
    31381 tcatggcgcg gtcctgatac aaaacggttc ccgccatcat acttcacaag gacttcactt
    31441 tcagacaata gtcttttgcc atcgtgatga atgtgagtga aaagatggaa catttcgacg
    31501 tggcgattat tggcctcggc ccggcagggt cggcgttggc acgaaagtta gccggcaaaa
    31561 tgcaggtgat cgcgctggat aaaaagcacc agtgcggtac tgaaggtttc agcaaacctt
    31621 gcagattttc agcgtcaaaa ctgtcgacgt cgccgcatcg ctcacgcgta actaccagcg
    31681 aagctatatc aatattaatc gccatgcttt cgacttgtgg atgaaatcgc tgatccccgc
    31741 cagcgttgag gtttatcacg atagcctgtg ccgaaaaatc tggcgtgagg atgataaatg
    31801 gcatgtcatt tttcgtgcag acggctggga gcaacatatt accgcccgct atctggtcgg
    31861 tgccgatggc gcaaactcga tggtgcggcg acatctctac ccggatcatc aaatccgtaa
    31921 atatgtcgct atccagcagt ggttcgcaga gaaacatccg gtgccgttct actcctgcat
    31981 ctttgataat gcgataactg actgttactc atggagtatc agcaaagatg gttattttat
    32041 ctttggcggt gcctatccaa tgaaagacgg tcagacgcgt ttcacgacgc tgaaagagaa
    32101 aatgagcgcc tttcagttcc agtttggcaa agcggtgaaa agtgagaaat gcacggtgtt
    32161 gtttccctca cgctggcagg attttgtctg tggtaaggac aacgcctttc tgattggtga
    32221 agcggcggga tttatcagcg ccagctcgct ggaggggatt agctatgcgc tggatagcgc
    32281 agagattctg cgtgcggtgt tcctgaaaca gccggagaag agcaacgccg cttactggcg
    32341 cgccacacgc aaactgcgtt taaaactctt cggcaagata gtaaaaagcc gatgcctgac
    32401 cgcaccggct ttaagaaagt ggattatgcg cagtggtgtg gcgcatattc cacagttgaa
    32461 agattatcca acgcgcttca catcacccac cagcaggatg taagagagcg cgccaatcaa
    32521 cgcgacggcg gagatataaa ccagtgcagg cgcgaaaccg taaccctgcg ccaggtaccc
    32581 caccaccagc ggaacggtga tgccgcccag accaccggcg aagttaaaca cgccaccggt
    32641 taaaccaatc aggcgcatcg gtgccagaga agagaccagc gaccaggtaa tcgaagcaaa
    32701 accgttgccg aagaatgcca gcgccatcag gcacataatc atcatcggat cgttggtgta
    32761 gttagcgccc ataatgcagg tggagatcag caagccgcag ataatcggcg ttttacgcgc
    32821 aaagcccagt gaaaagccct tacgtaccag cagatccgcg acccagccag agagcaggac
    32881 gccgacaaac gccgcgagga atggcaccgt ggtcataaag cctgctttca gcgccgtgat
    32941 tcctttttcc tgggttaaat agttcgggaa ccaggttaag aaaaaccaca gtgcagaagc
    33001 caccgcaaat tgcccaagat aaacgccgat cagtttacga tggaacacca gtttccagtc
    33061 tttggctgtt aacggctgac gcgcctcttt cttcaccggc gcatcaccat ccaccagacc
    33121 gccgccatca cgaatgtaat ccagttcagc tttgctgata cctttggtca ggcgcggcgg
    33181 ctgataaacc ttaaaccaaa tcagcgacca gataatgccg ataccaccag tgacaatgaa
    33241 cacccagtgc cagctcaaca tctcctgaat ccagatcagc agcggagtca aaaacgccag
    33301 accgacaaac tgaccagacg tataaaaacc aacggcggaa gcgcgttcat gttccggaaa
    33361 ccagctggtc accatccggt tattggtcgg gaaagcgggc gcttcgaaaa taccggttat
    33421 cgcacgcaga ccaattaatg acattaatcc cgtggcaaag ccctggaata aagtcgccac
    33481 tgaccaacca aatatcgcaa taaaataagt cacgcgagaa cctacgcgat ctaaaaacca
    33541 accgccgggg atctggcata gcgtataaag ccaggcgaag gccgaaaata catagcccat
    33601 ttccgctttc gtaatgccga actcttcctg aatatgggcg gaagccacgg ccaggttagc
    33661 gcggtcaaca taacaaatga ccaccgtaat aaagatcatc accagcgtca gataacgccg
    33721 acgccccggc tttgctgcat taacgggaat atccatagcg agctttctcc agattttggg
    33781 catagcgaag ccgctcacca tgccctgtaa tttacagagg gttatatttt tgtattgctg
    33841 ttttagtgcc cgatgagggg cttacgtggc aggaattacc actctgctac gctgttatct
    33901 tcatgacgcc agagcggatt acgccagtcc ggggcatttt tactgaactc aataacttta
    33961 gcttcgtcga tttccacacc taagcccggt ttcgttaacg gtttaaagaa gccgccaacc
    34021 atgctgaagt cttctttgtt tttcacaaag tcgagtaact ccgcgccttt gttgtaatga
    34081 atgcccatac tttgttactg aagtaccgcg ttataggaaa caaagtcgat atgcaggcaa
    34141 gccgccagtg caatcggtcc gaacggacag tgcggcgcaa gggtcacatc ataggcttct
    34201 gccattccag caattttgta gcattcggta ataccgcctg catgggagag atccggttgc
    34261 agaatcgaaa taccgcctgc ctccagcacg cgtttaaaat cgaaacgtga gaacatgcgc
    34321 tcacccgccg ccagtggaat atgcgtttgt gccgccaatt tcgggtagta ttcggcttgt
    34381 tccgccagca ccggctcttc aataaacagc gggcgatacg gctccagttc tttaatcagc
    34441 actttcgcca ttggcgcgct gacgcgaccg tggaaatcaa gaccaaactc aatctgattg
    34501 ccaaaagctt cacgaatttg tgccacggtg ttgactgccg catctaccgc gcgggagtta
    34561 tcaattagcc ccagttcttc acaaccgttc agtttgaagg tatcgaagcc gatttcgcgc
    34621 agggttttaa tgccgtcgat aacatccgcc ggacgatcgc cgccgaccca actgtaggct
    34681 ttaattttgt cgcgaaccag gccgcccatc agttgccaga ctggcgcatt cagcactttg
    34741 cctttgatat cccataacgc ctggtcgatc ccggcgatgg cgctcatcag gattggaccg
    34801 ccacgataaa atccggcgcg atacatcact tgccataagt cgttgatgcg cgaaggatcc
    34861 tgaccaatca aatagtcacc cagctcgtga actgccgctt ccaccgtacg ggcgcggcct
    34921 tcaatcacgg gctcgcccca accgaccacg ccttcatcgg tttcaatttt caggaacatc
    34981 cagcggggag gtaaacgata cgtggtaatt ttggtgattt tcattgcact gcctctcgat
    35041 acgccttaac aaatgctgct gcctgctgcg cggtgcgttc tacagactgc ccggcgcgat
    35101 aaagatcgct gcctaagccc gccccaacac aacctgcgtc tatccactgc gccaggtttt
    35161 ctggcgtcac gccgccaacg gcaaagactg cgatgtccgg tggtaatacc gcttttaacg
    35221 ctttgatgta ttgcggacca aaagccgatg acggaaatat tttcagcgcc tgcgcgcccg
    35281 cttcgagcgc ggtaaaggct tcggtcgcca tcgcgcagcc ggggcaaacg gtcatgccgt
    35341 agcccaccgc gcggcggatc acttcactat ggatattggg cgtaacgatg agctgacagc
    35401 ccatcctggc gagcgcatcg acctgttcag gtttcagtac cgtacctgcg ccaatcaacg
    35461 ccttgtcgcc atacgcatca acgatggcgg gaatgctctg ctcccattgt ggggaattca
    35521 gcgggatttc aaccgcgtcg aacccggcgt caatcaccgc gccaacatgc gccagcgcct
    35581 cgtcgggcgt aatgccgcgc aaaatggcga tcagcgggag tttagtttgc cactgcatga
    35641 gcgatgctcc ttataccagc ctgaaatgcc gtgtcgcccg ccaccgccgc cacgtcgcaa
    35701 cccatcgcct gaaaggcttg ctggtagcgc gcggtcagcg atgttccggc gacaagggtg
    35761 atggcgtgtt gatgggtcac atagtcgcgc atactggcaa cctctgcgcc aatcaacaaa
    35821 ccagagagaa tgttcgcgga gaagtgttcc cagcacatgc gaggcgcgaa cttcaaaaag
    35881 ctgcggcaat atggcgggcg cattaaggcc gcgctcaagg ccagccgcga aggcatcggc
    35941 agagttttcc tgcggcggca aacctgcgcc aatcaatgag tgatttaaca gtaaatgatg
    36001 taattcaccg gtcatcacgg tgcgaaaatc gttgatttgc tggctatcgg cctgcaccca
    36061 tttgcaatgc gtaccgggca taacataaag agaggaagga gccagagctc gcgcgccgat
    36121 caattgtgtt tcttcgccgc gcatcacatt gtggttatcg tcatgagaga cgcataatcc
    36181 gggaataatc cagatattgt cgccaacaga cgttaattgt tcgccaatag acgaaaaacg
    36241 ggcaggaaca gataaatacg gagcaacttt ccagccgaca ttgctgccga ccattcctgc
    36301 catcaccact ggcgtatttt cttcacgcca gtcggtcgtg acttctgcta acaccgcagc
    36361 cggagatttt ccgttcaggc gcgtgacgcc tgcttctgat tgcctgctct ccaggcagtg
    36421 gtcgccctga taaagccagg cgcgcagatt ggtcgatccc cagtcaattg cgatgtagcg
    36481 agctgtcatg tgatttcctt taaccttcgt gtcgagctgg caatcatggt aagcgccgcc
    36541 tgctctgccg catcgccgtc ctgatgccgt atcgcatcga acagcgcctt atgttcctgg
    36601 agcgtttgcg gcatgttggc ctcatcgccc atccaggttc gttcaaaaac cgcccgctgc
    36661 agcgaactga tcgcaatgct aagttgctgt aacaccgggt tatgcaccga ctgcagcacc
    36721 gcctcgtggt agcgaatatc cgcttcgtta aacgcttcgc ggtcctgatt gttggcaatc
    36781 atctcgttca gcgccgattc aatctgcgcc agatcgctgg aggtcgcgcg ctctgctgcc
    36841 caacgggcaa tcgccggttc caccagattt cgcacttcgc tcatggcact gataagccgt
    36901 gggtcgtagt cgttttccag cacccattgc agtacgtcag tgtcgaggta attccactgg
    36961 ttacgcggtg ccacaaacgc cccgcgataa cgtttcattt caatcagccg cttcgccatc
    37021 agcgaacgga acacctcacg gatgatgttg cgcgaggttg caaactcttc acagagctcc
    37081 gcctcagccg gaagtggcga gccaggtacg tatttgccgt gaacgatctg tttacccagc
    37141 gtaatgacaa tgcgatcggt tttattgaga gtcatggaga gtccttgtgc tctgtatgtt
    37201 cttctctact ttaccccgat cgatgcataa cgcggcaact ttgtagtacc agcgtgatga
    37261 cgttcgcgtt tgccgtgcgt gtaatgtagt acaaacttat attgttgtgc tacaatttag
    37321 atcaaaaaaa gaacaatgca taaaaaaatg acatgcgtcg ggcagaaatc tgaaaaggga
    37381 tatcaggcgc taaacaggag ggaaagaaga gtatgctttc aacggcttag ctactcgttt
    37441 aaaagattaa tcatgaagtt gaattttaag ggatttttta aggctgccgg tttattccca
    37501 ctggcgctga tgctttcagg ctgtatctcg tatgctctgg tttcccatac cgcaaagggt
    37561 agttcaggaa agtatcaatc gcagtcagac accatcactg ggctatcgca ggcaaaagat
    37621 agtaatggaa caaaaggcta tgtttttgta ggggaatcgc tggattacct tatcactgat
    37681 ggtgccgatg acatcgttaa gatgctcaat gatccagcac ttaaccggca caatattcag
    37741 gttgccgatg acgcaagatt tgttttaaat gcggggaaaa agaaatttac cggcacaata
    37801 tcgctttact actactggaa taacgaagaa gaaaaggcac tggcaacgca ttatggtttt
    37861 gcctgtggtg ttcaacactg taccaggtca ctggaaaacc taaaaggcac aatccatgag
    37921 aaaaataaaa acatggatta ctcaaaggtg atggcgttct accatccatt taaagtgcga
    37981 ttttatgaat actattcacc cagaggcatt ccggatggtg tttccgcagc attactgcca
    38041 gtgactgtta cgctggacat cattactgca ccgctgcaat ttctggttgt atatgcagta
    38101 aaccaataat cagtaagcgg gcaaacgcgt ttatgctgtt tgcccgccca cagattaatt
    38161 cagcacatac ttctcaatag caaacgccac gccatcttca aggttggatt ttgtgacaaa
    38221 gttcgccatt tctttcaccg aaggaatagc gttacccatc gccacaccga cgcctgcata
    38281 ttcaatcatt gcgatgtcgt tttcctgatc gccaatcgcc atgatttctt ccggtttaat
    38341 acctaacacg tcggccagtg atttcacacc cgtacctttg ttaacgcgtt tatcgaggat
    38401 ttcgaggaag tacggcgcac ttttcagcac ggtatatttc tctttcactt cctgcggaat
    38461 acgcgcgata gcctggtcga ggatggcggg ttcatcaatc atcatcactt tcaggaactg
    38521 ggtattgggg tccattttct ccgcttcgca gaacaccagc ggaattgtgg caacgaagga
    38581 ttcatgcacc gtgtagtagc tgatatcgcg gttagcggtg tacagcgtgg tgcggtccag
    38641 ggcgtggaaa tgagaaccga cttcgcgaga gagtttttcc aggaaacgat agtcgtcata
    38701 gctgagagcg gtttgcgcca cggtgctacc atcagcggcc ttctgtacca gcgcgccgtt
    38761 ataagtaatg cagtagtcgc ccggctgttc catatgcagc tctttcaggt agttgtgcac
    38821 acctgcatac ggacgacccg tcgttagcac gacattcacg ccacgggcgc gagctgcggc
    38881 aatcgcattt ttaacggcgg gtgaaatggt gtgatcgggc agcagaaggg tgccatccat
    38941 atcgatagca atgagtttga tagccatgag ttcccaaggt agattagttc ctgacccatg
    39001 ctaacgcgat tccgctcaaa aatcagtaca acacccgagg gaaaagggga tgcaacgcgg
    39061 gttgcatgct ccccatttgc ttagcggaag agtttccctt tcagcagttc catgcctgcg
    39121 gaaagcagat cgttattggc ttgtgctgac acttcacctt gcggtgagag cgcatcaata
    39181 atcttcggca attgctctgc cagtaaacag gaagccgtac tggtatccac gccaagtttt
    39241 tgcccgagat cggacaccgc atttgtgccg agcgccgatt ccagttgctc gccgctaact
    39301 gattgatttc tctgttgatt actcaaccaa gttgagagaa tggcccctaa gccgccactt
    39361 tgcagttttt ccagcagcac ctgaatgcca ccctgctcct caacccaact taaaatcgcc
    39421 tgatatttcc ccgcatcgcc tttcagaaag gcaccgacaa cttcatcaaa aagccccatg
    39481 ataatcacct gtaaagcgtt acgtgttgac ccaaaaagta tagatctgcg ggatgataat
    39541 tgcggattgc agaaataaaa agggcggaga tgatctccgc ccttttctta tagcttcttg
    39601 ccggatgcgg cgtgaacgcc ttatccggcc tacaaaatca tgcaaaatca atatattgca
    39661 ggagtctcgt aggactaata agcgtagcgc atcaggcacg ctcgcatggt taacgccatt
    39721 aaatatcaat attcgccgct ttcagggcgt tctcttcaat aaacgcacga cgcggttcta
    39781 cggcgtcgcc catcagcgtg gtgaacaact ggtcagcggc aatcgcatcc ttaacggtaa
    39841 cgcgcagcat acgacggctt tccgggtcca tggtggtttc ccacagctgt tccgggttca
    39901 tctcgcccag acctttataa cgctggatgg agaggccacg acgggactct ttcaccagcc
    39961 agtccagcgc ctgctcgaag ctggctaccg gctgacgacg ctcgccacgt tcgataaacg
    40021 catcttcttc cagcaagcca cgcagtttct cacccagcgt gcagatacga cgatattcgc
    40081 caccggtgat aaactcgtga tccagcggat agtcagtatc cacaccgtgg gtacgcacgc
    40141 gaacaatcgg ctcgaacagg ttttgttcgg cattggtgtg gacatcaaac ttccactggc
    40201 tgccgtgctg ttctttgtcg ttcagttcgc tgaccagcgc gttcacccag cgggtaacgg
    40261 tctgctcatc agaaaggtca gcttccgtca acgtcggctg atagataagc tctttcagca
    40321 ttgctttcgg ataacgacgc tccatgcggt tgatcatttt ctgcgtcgcg ttgtactcag
    40381 ataccagttt ctctaacgct tcgccagcca atgccggtgc actggcgttg gtgtgcagcg
    40441 ttgcgccgtc cagcgcgata gagatctggt actgatccat cgcttcgtcg tctttaatgt
    40501 actgttcctg cttgcctttc ttcactttgt acagcggcgg ctgagcgatg tagacgtgac
    40561 cgcgttcaac gatttccggc atctgacgat agaagaaggt caacagcagc gtacgaatgt
    40621 gcgagccgtc gacgtccgca tcggtcatga tgatgatgct gtgataacgc agtttgtccg
    40681 ggttgtactc gtcacgaccg ataccacagc caagcgcggt gataagcgtc gccacttcct
    40741 gagaagagag catcttatcg aagcgcgctt tctcgacgtt aaggatttta cccttcagcg
    40801 gcagaatcgc ctggttcttg cggttacgcc cctgcttcgc agagccgccc gcggagtccc
    40861 cttccaccag gtacagttcg gaaagcgccg gatcgcgttc ctggcagtct gccagtttgc
    40921 ccggcaggcc agctaagtcg agcgcacctt tacggcgggt catttcacgc gcgcgacgcg
    40981 ccgcttcacg ggcacgggca gcatcgataa ttttgccaac cacgattttc gcatcagttg
    41041 ggttttccag caggtattcc gccagcagtt cgttcatctg ctgttcaacc gccgatttca
    41101 cctcagaaga aaccagtttg tctttggtct gggaggagaa tttcgggtcc ggcactttta
    41161 cggaaacgac cgcaatcagg ccttcacgcg catcgtcacc ggtggcgctg actttggctt
    41221 ttttgctgta gccttctttg tccatgtagg cattcagggt acgggtcatc gccgcacgga
    41281 agcctgccag gtgagtaccg ccgtcacgct gcggaatgtt gttggtaaag cagtagatgt
    41341 tttcctggaa gccatcgttc cactgcagcg ccacttcaac gccaataccg tctttttcgg
    41401 tggagaagta gaagatattt gggtggatcg gcgttttgtt cttgttcaga tattcaacga
    41461 acgctttgat gccgccttca tagtggaagt ggtcttcttt gccgtcacgc ttgtcgcgca
    41521 gacgaatgga aacgccggag ttgaggaacg acaactcacg cagacgtttc gccagaattt
    41581 catattcgaa ctcggtcaca ttggtgaagg tttcgaggct gggccagaaa cgcaccatgg
    41641 tgccggtttt ttcagtctcg ccggtaaccg ccagcggagc ctgcggtaca ccgtgttcgt
    41701 agatctgacg gtgaatttta ccctcgcgct gaataaccag ctccagtttt tgcgacaggg
    41761 cgtttactac cgaaacacca acgccgtgca gaccgccgga cactttatag gagttatcgt
    41821 caaatttacc gcctgcgtgc agaacggtca tgatcacttc cgccgccgat acgccctctt
    41881 ccgggtgaat accggtcgga atgccgcgcc cgtcatcctg tacagagaca gagttatcgg
    41941 cgtgaatggt gacgataatt tctttacagt gacccgcgag cgcttcgtcg atagcgttat
    42001 ctaccacctc gaataccatg tggtgcagac cggtgccgtc atccgtgtcg ccgatataca
    42061 tacccgggcg cttacgcacc gcatccagcc ctttcaggac tttgatactg gaggagtcat
    42121 aagaattcga catcaacgtt tctcgctcat ttatacttgg gttaatccgt tattttaccc
    42181 ttttccacgg taaacatctt cgaattttcg tccgacatgt ctataacgtg ttcagcactg
    42241 atcgcgctga caaagacctg tgattgcgtc gcttttaagc ggctggcaag caacccgcga
    42301 cgctcatcat caagctcaga ggcaaaatca tctatcaggt agagacaccg ccgcccgctt
    42361 tcacgagtga ggaactctcc ttgcgccaga cgtaaggcgc acatcaacag cttaagctgc
    42421 ccacgcgata aggtatcttc caccggcgca ccgtcggcgc gaatgcgtaa gtccgcttta
    42481 tgcgggccat gcgcggtgta ggttagctgg cgatcgcgtt caaaattacg ttccagcacc
    42541 tcagcgtact ctgtctcttt ctcccagccg cgctggaaag agaaagtcag agaaaactca
    42601 gggagaaatt gcttacaggt atcatccata tcggccgcga taccggcgct atactccgcg
    42661 cgccaggtgc tgatttgctc cgccagcggg atcagttctt tatcccacgg gcgtagctgt
    42721 tcgtaacgag tcacctggcg cagcgccgca ttgcgctgct tgagcaatcg cttgagattg
    42781 ctccaggcgg tgaaaaatcc aggttcgttg tgaaagcatc cccagtcgag gaatgctctt
    42841 ctgtatttgg ggccgccgtt gagtaaagta aacccttctg gcgttatcaa ctgcatcggc
    42901 atcaggtgcg ccagttccgc gactttatgc ccgtccgtac cgtcaatacg gactttgctg
    42961 tcgccctgtt tgtctttggt taagccaatc gccgtctcgc gctcttcgcc ctgtaatcgc
    43021 ccgtggagaa caaatgcctc ctgctcatgg cgaatgacgc gaccaatctg caaactgcga
    43081 aacgcccgac catggccgag cgtatagatg gcttccagca cgctggtttt gccactgccg
    43141 ttggcaccta ccagaaagtt aaagccggga gataaagcga gatccgcggt ttcaatattg
    43201 cggaaatcgc ggatcaacaa gcgggtgagg gacattacag tctcattggc atgacaacat
    43261 aggcagccga ctgtgatgcg gcatcttcaa tctgcacgct ggaaaccgaa tcggtcagca
    43321 tcatgcggac gttttcgcat ttcagcgcgt tcagaacatc cagcacatag ctgacgttga
    43381 agccgatttc catctccgca ccgctatagg taacgtcgag gatctcttcc gcttcttcct
    43441 gttccgggtt gttggcagta attttcagct ggttttcgct gacatacagg cgcacaccgc
    43501 ggaatttctc gttagagaga atcgccgcgc gagcaaacgc ctgcttgagc agatcgcagc
    43561 ctgcttccag atgtttgtcc gggttcttcg gcagaacgcg acggtaatcc gggaagcgac
    43621 catccaccag tttcgaggtg aaaataaagt cgccaacgtg ggcacgaatg ttattgctgc
    43681 caatctgcac acgcagcaga ttgtcgccgc cgtcgagcat acgcatcagt tcaatcacgc
    43741 ctttacgcgg tacgatcacc gaatggcttg gcaaagattg accaattggc attgaacaga
    43801 ccgccagacg gtggccgtcg gttgccacgg tgcgcagttc ttcaccttcg gtttcaaaca
    43861 gcataccgtt taagtaatag cgaacatcct gatgcgccat cgaaaactgg gttgcttcaa
    43921 tcagacgctt catcgtcgcc tgcggcaggg taaattcgac ttcgctctgc cagtcatcga
    43981 ggttcgggaa atccgccgct ggcagggtag acagcgaaaa acggctacgc ccggagcgca
    44041 ccagcatccg ttcaccttcc agctgcacgg caatttccgc gccttcaggc agaccacggc
    44101 agatatcaaa gaatttgcgc gccggaacgg tcgtcgctcc tggctcgtgt ggctgaacca
    44161 gcgcaacgcg tgccaccatt tccatctcga gatcagtacc ggtcagcgac aacgtaccgt
    44221 cagcaacctg taacagcaga ttaccgagaa tcggtagcgt aggacgacca cctaacggac
    44281 cgctcacctg ttgtagcggt tttaataaat gctcacgttc tacggtaaat ttcataggtt
    44341 tacgatgaca atgttctgat taaatttgaa aaatcttctt tgatatcgtg gctctcttca
    44401 cgcaactgct cgatcttacg gcaggcatga agcaccgtcg tgtggtcacg cccaccaaac
    44461 gcatcgccaa tctctggcag actgtggtta gtcagctctt tcgctagcgc catcgccatc
    44521 tggcgcggac gcgccaccga gcgggatcga cgcttggaaa ggagatccgc gactttgatc
    44581 ttgtagtact ccgccaccgt cttctgaata ttgtcgatgg tgaccagttt ttcctgcaat
    44641 gccagcaagt cgcgcagcgc ctcacgcacg aagtcgatgg tgatcgcccg tccggtaaag
    44701 ttggcgttag caataacgcg gttcagcgcc ccttccagct cacgtacgtt agatcgtaga
    44761 cgcttggcga taaagaacgc cacttcaccc ggcaaacgaa tgtcattttc gtcggccttt
    44821 ttcatcagga tcgccacacg ggtttccagc tctggcggtt cgatcgccac agtcagtccc
    44881 caaccgaagc gggatttcaa acgatcctca acgccgttga tctctttcgg atagcgatcc
    44941 gaggtgagaa tgatctgttg attaccttcc agcagggcgt tgaaggtgtg gaaaaactct
    45001 tcctgagatc gttctttatt agcaaaaaac tgaatatcgt cgatcagcag tgcatctacg
    45061 gaacggtagt agcgtttaaa ctcttcgatc gcgttgtttt gcagggcttt aaccatgtcc
    45121 tgaacaaagc gctcggagtg catataaacc actttggcat tcggcttgcg cgccataatg
    45181 ccgttaccca ccgcatgcag caggtgagtt ttacccagac ccgtgccgcc ataaaggaac
    45241 aacgggttat aggcaccgcc agggttatcc gccacctggc gagccgccgc gcgcgccagt
    45301 tggttagatt taccttcaac gaagttatca aacgtgtgtt tgacgtttac gttagaacga
    45361 taggtcggtt ctgccggggc cgggacgtta tcccaacctg agcgcgtaga aggcgcagca
    45421 cgttgcggct gcgtttgcgc cacctgtgca ggggccgcga cgttgctcgt cactgccgct
    45481 tgtggcgttt gcgtcaccgg tttggtgccg acttcaaaac gcagctgtgg ggcatccgct
    45541 ccgcagaaac tggttagcag tccattgata ttattaaggt acttgtcccg tacccaatcg
    45601 aggacaaaac ggtttggcgc gtacagggac agcgtgttat cgctcagttc cgcctgcaat
    45661 gggcgtatcc acatactgaa ttctgtggct ggtaactcat cctgcaatcg ggcaagacac
    45721 tgctgccaaa gcgaaagtga cacggcggac tccactcgaa caaaagtcga taatgactaa
    45781 ggctgaaaca ttcatgattg ttgacgtacg tcgaaaagac cctgcttgag ggtgacgcac
    45841 gaaccgctgt ctgcggttat atgcccgatc aagatcctgc aaaacgatcg ggaccgcgga
    45901 tcatagccta aactgcgcaa gagatcttct gtttctcaca gattttccct atttatccac
    45961 aggactttcc agaactcgct aagtgtaaac gatcctgccg cgaggcgggc acgatttacg
    46021 ccgcatattg gaaaatttaa tgaccataga caaaaattgg cttaatcgat ctaataaaga
    46081 tccaggacga tccttgcgct ttacccatca gcccgtataa tcctccaccc ggcgcgccat
    46141 gctggtttcc actggtgtga ggtcgtacat tttccctgcg aaaaggtgcg gaaaagcgcg
    46201 gtaaataagg aaagagaatt gactccggtg tgtacaatta ttacaatccg gcctctttaa
    46261 tcacccatgg cttcggtgtc catcgtttca tttttcggcg gatatccaat aaagccattg
    46321 aatttattca agtttaggta gaaatcgcca tgaaacgcac ttttcaaccg tctgtactga
    46381 agcgcaaccg ttctcacggc ttccgtgctc gtatggctac taaaaatggt cgtcaggttc
    46441 tggcacgtcg tcgtgctaaa ggccgcgctc gtctgaccgt ttctaagtaa taaagctaac
    46501 ccctgagtgg ttaagctcgc atttcccagg gagttacgct tgttaactcc cagtcaattc
    46561 acattcgtct tccagcagcc acaacgggct ggcacgccgc aaattaccat tctcggccgc
    46621 ctgaattcgc tggggcatcc ccgtatcggt cttacagtcg ccaagaaaaa cgttcgacgc
    46681 gcccatgaac gcaatcggat taaacgtctg acgcgtgaaa gcttccgtct gcgccaacat
    46741 gaactcccgg ctatggattt cgtggtggtg gcgaaaaaag gggttgccga cctcgataac
    46801 cgtgctctct cggaagcgtt ggaaaaatta tggcgccgcc actgtcgcct ggctcgcggg
    46861 tcctgatagc cctcattcgg gtctatcaac gcctgattag tccgctactc gggccgcatt
    46921 gtcgtttcac tccaacctgt tcaagctacg gaattgaggc attgcgcagg tttggagtga
    46981 taaaaggcag ttggttgacg gtgaaacgcg tattaaaatg ccacccttta caccctggtg
    47041 gtgacgatcc cgtcccgccc ggaccatttg ataccagaga acactaacga tggattcgca
    47101 acgcaatctt ttagtcatcg ctttgctgtt cgtgtctttc atgatctggc aagcctggga
    47161 gcaggataaa aacccgcaac ctcaggccca acagaccacg cagacaacga ccaccgcagc
    47221 gggtagcgcc gccgaccagg gcgtaccggc cagtggccag gggaaactga tctcggttaa
    47281 gaccgacgtg cttgatctga ccatcaacac ccgtggtggt gatgttgagc aagctctgct
    47341 gcctgcttac ccgaaagagc tgaactctac ccagccgttc cagctgttgg aaacttcacc
    47401 gcagtttatt tatcaggcac agagcggtct gaccggtcgt gatggcccgg ataacccggc
    47461 taacggcccg cgtccgctgt ataacgttga aaaagacgct tatgtgctgg ctgaaggtca
    47521 aaacgaactg caggtgccga tgacgtatac cgacgcggca ggcaacacgt ttaccaaaac
    47581 gtttgtcctg aaacgtggtg attacgctgt caacgtcaac tacaacgtgc agaacgctgg
    47641 cgagaaaccg ctggaaatct cctcgtttgg tcagttgaag caatccatca ctctgccacc
    47701 gcatctcgat accggaagca gcaacttcgc actgcacacc ttccgtggcg cggcgtactc
    47761 cacgcctgac gagaagtatg agaaatacaa gttcgatacc attgccgata acgaaaacct
    47821 gaacatctct tcgaaaggtg gttgggtggc gatgctgcaa cagtatttcg cgacggcgtg
    47881 gatcccgcat aacgacggta ccaacaactt ctataccgct aatctgggta acggcatcgt
    47941 cgctatcggc tataaatctc agccggtact ggttcagcct ggtcagactg gcgcgatgaa
    48001 cagcaccctg tgggttggcc cggaaatcca ggacaaaatg gcagctgttg ctccgcacct
    48061 ggatctgacc gttgattacg gttggttgtg gttcatctct cagccgctgt tcaaactgct
    48121 gaaatggatc catagatttg tgggtaactg gggcttctcc attatcatca tcacctttat
    48181 cgttcgtggc atcatgtacc cgctgaccaa agcgcagtac acctccatgg cgaagatgcg
    48241 tatgctgcag ccgaagattc aggcaatgcg tgagcgtctg ggcgatgaca aacagcgtat
    48301 cagtcaggaa atgatggcgc tgtacaaagc tgagaaggtt aacccgctgg gcggctgctt
    48361 cccgctgctg atccagatgc caatcttcct ggcgttgtac tacatgctga tgggttccgt
    48421 tgaactgcgt caggcaccgt ttgcactgtg gatccacgac ctgtcggcac aggacccgta
    48481 ctacatcctg ccgatcctga tgggcgtaac gatgttcttc attcagaaga tgtcgccgac
    48541 cactgtgacc gacccgatgc agcagaagat catgaccttt atgccggtca tcttcaccgt
    48601 gttcttcctg tggttcccgt caggtctggt actgtactat atcgtcagca acctggtaac
    48661 cattattcag cagcagctga tttaccgtgg tctggaaaaa cgtggcctgc atagccgcga
    48721 gaagaaaaaa tcctgattcg gtgagttttc gctaaaataa gggcggtcag ttgaccgcct
    48781 tttttctttt cgtagggcgg ataagcaccg cgcatccgcc acacaaagca acaggaacat
    48841 catgagcgat aatgacacta tcgtagccca ggccacacct ccgggacgtg gcggcgttgg
    48901 catcctgcgc atctccggct tcaaagcccg tgaagttgcc gaaaccgtgc tgggtaaact
    48961 gcctaagccg cgctacgccg attatcttcc gtttaaagac gccgacggca gcgtgctcga
    49021 tcaggggatt gcgctatggt tccctggccc gaactcgttc accggcgaag atgtgctgga
    49081 actgcaaggt catggcggtc cggtgatcct cgacctgctg ttaaaacgca ttctgaccat
    49141 tcccggcctg cggattgctc gccctggtga gttttccgaa cgcgcgtttc ttaacgataa
    49201 acttgactta gcccaggccg aggcgattgc cgatcttatc gacgccagtt cggaacaggc
    49261 ggcccgttcg gcacttaact cgctgcaagg cgcattctcc gcacgggtta atcatctggt
    49321 agaagccctc acccacttgc gcatttacgt cgaagcggca attgatttcc ctgatgaaga
    49381 gatcgatttc ctctccgacg gcaaaattga agcccagctt aatgacgtga tggccgatct
    49441 cgatgcagtg cgtgctgaag cacgtcaggg tagtttgttg cgcgaaggga tgaaagtagt
    49501 gattgccgga cgtcctaatg ccggtaaatc gagcctgtta aacgcgctgg cgggccgtga
    49561 agcggcaatc gtaaccgata tcgccggaac cacgcgtgac gtgctgcgtg agcatatcca
    49621 cattgacgga atgccgctgc atatcatcga cactgccggg ctacgtgagg ccagtgacga
    49681 agtggaacgt attggtatcg agcgcgcgtg gcaggaaatt gaacaggccg accgcgtgct
    49741 gtttatggtc gatggcacca cgacagacgc cgttgatccg gcagagatct ggccggaatt
    49801 tatcgcccgt ctgcccgcga aactgccgat caccgtggta cgcaataaag ccgatatcac
    49861 cggcgaaacg ctgggaatga gtgaagtgaa cggtcacgcg ttaattcgtc tctcggcaag
    49921 gactggtgaa ggcgtggacg tgctgcgtaa ccatctcaaa cagagcatgg gctttgacac
    49981 caatatggaa ggcggcttcc tggcgcgccg tcgccactta caggcgctgg aacaggcagc
    50041 ggagcatttg cagcaaggta aagcgcaact gttgggagcc tgggcaggtg aactgctggc
    50101 ggaagagttg cgcctggcac agcagaactt aagcgaaatc accggggaat ttacttcaga
    50161 cgacctgctg gggcggattt tctccagctt ctgtattggt aagtaaccgc gcttacgaag
    50221 ccgtattctg actgtcagat gcggcttcgc ttcattgtta gcactcctgt tatgcctcaa
    50281 ccctttttta aacattaaaa ttcttacgta atttataatc tttaaaaaaa gcatttaata
    50341 ctgctccccg aacgattgtg attcgattca catttaaaca atttcagaat agacaaaaat
    50401 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
    50461 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
    50521 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
    50581 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
    50641 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
    50701 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
    50761 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
    50821 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
    50881 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
    50941 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
    51001 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
    51061 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
    51121 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
    51181 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
    51241 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
    51301 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
    51361 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
    51421 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
    51481 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
    51541 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
    51601 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
    51661 tatggcggcc tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
    51721 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
    51781 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
    51841 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
    51901 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
    51961 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
    52021 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
    52081 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
    52141 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
    52201 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
    52261 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
    52321 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gagtgccttt
    52381 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
    52441 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
    52501 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
    52561 atctctgcta atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct
    52621 aatccacgta ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt
    52681 tcgttagccg ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg
    52741 atcgtgtttg gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc
    52801 accactgcgg gaacgtctta cttcccgtat atctttatgg ctttgccggt gtgtctggcg
    52861 tcatttggtt tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat
    52921 aagttaatca aaagcgtggt atttggttcg ctgctggcgc tggtgattta tctcttctgg
    52981 ctctattgca cgatggggaa tattccgcgc gaaagcttta aggcgataat ctcctcaggc
    53041 ggcaacgttg attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag
    53101 ttttgcctgc tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg
    53161 gggttgttcg attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc
    53221 aaaaccgtgc tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc
    53281 tttatttacg ggatcggcgg tgccaggctg tgcgccacca tctgggcggt cattattccc
    53341 gcagtgcttg caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc
    53401 ggcaatctta ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc
    53461 ggcaacgtct ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg
    53521 ctggcttctt gcctctcagg aaatcactta tgtccaaatg gcaactcgcc ttatcctcct
    53581 tcaccacgta tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt
    53641 tgatttgtag ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg
    53701 gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct
    53761 ccgtatatct ggcggggatg gcagctgcga tgctatttgc cggtaaagtg gccgatcgtt
    53821 cagggagaaa gccggtcgcc atacccggcg cggcgctatt tattattgcc tcggtgttct
    53881 gttcactggc tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg
    53941 caggctgttg ttacgtggtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc
    54001 gggctaaagt gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag
    54061 tgctcggaca tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa
    54121 tgatgggcat cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt
    54181 cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cgttttttcc
    54241 tcagccgtgt tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat
    54301 cgccggtatt gctgatggaa atcatggggt ttgagcgcgg agaatacgcc accattatgg
    54361 cgctgaccgc tggcgtcagc atgacctttt cattctcgac gccatttgcg ctgggaattt
    54421 ttaagccacg tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc
    54481 ttgccgtttc accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt
    54541 tctcggtagg ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg
    54601 gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc
    54661 tggccgcggt ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta
    54721 gcatagtgag cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg
    54781 aagaaatcca tcaccacgct tgatctcaat ctgctgctct gtctgcaact gctgatgcag
    54841 gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag
    54901 tcgctggcaa agttaagagc gtggtttgac gacccgctct ttgtgaactc accgctgggg
    54961 ctgtcgccca cacagctaat ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg
    55021 agcaatctgc tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagttg
    55081 gcggcggaat cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa
    55141 cgttacccgc aggcgaccat caaactacgt aactgggatt acgattcctt agatgccatt
    55201 actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag
    55261 ctgttaagct cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtaccctgc
    55321 gtctggttac gcaaagatca tccggcactg catgaaacgt ggaatctgga caccttttta
    55381 cgttatccgc atatcagcat ttgctgggaa cagagcgaca cct
//
LOCUS       DADMEQ010000015        53210 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.015, whole genome shotgun sequence.
ACCESSION   DADMEQ010000015 DADMEQ010000000
VERSION     DADMEQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.015"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(11..322)
                     /locus_tag="JNZ05_000917"
     CDS             complement(11..322)
                     /locus_tag="JNZ05_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HAY8564239.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="JNZ05_000918"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="JNZ05_000918"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="JNZ05_000919"
     CDS             701..1054
                     /locus_tag="JNZ05_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HAY8564240.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="JNZ05_000920"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="JNZ05_000920"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HAY8564241.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FINVIPYSSWQIDAAIIGNAHNVVITSSDKIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="JNZ05_000921"
     CDS             complement(2810..3280)
                     /locus_tag="JNZ05_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564242.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="JNZ05_000922"
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="JNZ05_000922"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HAY8564243.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="JNZ05_000923"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="JNZ05_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY8564244.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="JNZ05_000924"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="JNZ05_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HAY8564245.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="JNZ05_000925"
     CDS             complement(5283..8432)
                     /locus_tag="JNZ05_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAY8564246.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAVSLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="JNZ05_000926"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="JNZ05_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HAY8564247.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="JNZ05_000927"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="JNZ05_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HAY8564248.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="JNZ05_000928"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="JNZ05_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HAY8564249.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQVQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="JNZ05_000929"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="JNZ05_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HAY8564250.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="JNZ05_000930"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="JNZ05_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HAY8564251.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREAAAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="JNZ05_000931"
     CDS             14911..15288
                     /locus_tag="JNZ05_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HAY8564252.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="JNZ05_000932"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="JNZ05_000932"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAY8564253.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="JNZ05_000933"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="JNZ05_000933"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAY8564254.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWTAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     gene            18099..18428
                     /locus_tag="JNZ05_000934"
     CDS             18099..18428
                     /locus_tag="JNZ05_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAY8564255.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="JNZ05_000935"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="JNZ05_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAY8564256.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="JNZ05_000936"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="JNZ05_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAY8564257.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="JNZ05_000937"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="JNZ05_000937"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAY8564258.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="JNZ05_000938"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="JNZ05_000938"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HAY8564259.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23893)
                     /gene="aes"
                     /locus_tag="JNZ05_000939"
                     /pseudo
     CDS             complement(22933..23893)
                     /gene="aes"
                     /locus_tag="JNZ05_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24045..25349
                     /locus_tag="JNZ05_000940"
     CDS             24045..25349
                     /locus_tag="JNZ05_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HAY8564260.1"
                     /translation="MKFPGKRKSKNYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(25482..27158)
                     /locus_tag="JNZ05_000941"
     CDS             complement(25482..27158)
                     /locus_tag="JNZ05_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HAY8564261.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIGVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27396..28616)
                     /locus_tag="JNZ05_000942"
     CDS             complement(27396..28616)
                     /locus_tag="JNZ05_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8564262.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALPGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMISGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28834..30486
                     /locus_tag="JNZ05_000943"
     CDS             28834..30486
                     /locus_tag="JNZ05_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY8564263.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="JNZ05_000944"
     CDS             complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="JNZ05_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HAY8564264.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31206..32000)
                     /locus_tag="JNZ05_000945"
     CDS             complement(31206..32000)
                     /locus_tag="JNZ05_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564265.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALLVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32138..32479
                     /locus_tag="JNZ05_000946"
     CDS             32138..32479
                     /locus_tag="JNZ05_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY8564266.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32694..35198)
                     /gene="copA"
                     /locus_tag="JNZ05_000947"
     CDS             complement(32694..35198)
                     /gene="copA"
                     /locus_tag="JNZ05_000947"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HAY8564267.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35460..36392
                     /gene="glsA"
                     /locus_tag="JNZ05_000948"
     CDS             35460..36392
                     /gene="glsA"
                     /locus_tag="JNZ05_000948"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAY8564268.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36395..37687
                     /locus_tag="JNZ05_000949"
     CDS             36395..37687
                     /locus_tag="JNZ05_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8564269.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37812..38219
                     /gene="cueR"
                     /locus_tag="JNZ05_000950"
     CDS             37812..38219
                     /gene="cueR"
                     /locus_tag="JNZ05_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HAY8564270.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLMTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38220..38678)
                     /locus_tag="JNZ05_000951"
     CDS             complement(38220..38678)
                     /locus_tag="JNZ05_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HAY8564271.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTYVEVIAIEGITLHIRAVSS"
     gene            complement(38675..39592)
                     /locus_tag="JNZ05_000952"
     CDS             complement(38675..39592)
                     /locus_tag="JNZ05_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HAY8564272.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39738..40415
                     /gene="fetA"
                     /locus_tag="JNZ05_000953"
     CDS             39738..40415
                     /gene="fetA"
                     /locus_tag="JNZ05_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HAY8564273.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EVRYELA"
     gene            40402..41181
                     /gene="fetB"
                     /locus_tag="JNZ05_000954"
     CDS             40402..41181
                     /gene="fetB"
                     /locus_tag="JNZ05_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HAY8564274.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="JNZ05_000955"
     CDS             complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="JNZ05_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HAY8564275.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="JNZ05_000956"
     CDS             complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="JNZ05_000956"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY8564276.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42982..43580)
                     /gene="tesA"
                     /locus_tag="JNZ05_000957"
                     /pseudo
     CDS             complement(42982..43580)
                     /gene="tesA"
                     /locus_tag="JNZ05_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43551..44237
                     /locus_tag="JNZ05_000958"
     CDS             43551..44237
                     /locus_tag="JNZ05_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8564277.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44234..46648
                     /locus_tag="JNZ05_000959"
     CDS             44234..46648
                     /locus_tag="JNZ05_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8564278.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTDDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFVG"
     gene            complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="JNZ05_000960"
     CDS             complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="JNZ05_000960"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HAY8564279.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQATIAVVEDPFEIRLERLNEEYFFRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48523..49449)
                     /gene="allS"
                     /locus_tag="JNZ05_000961"
     CDS             complement(48523..49449)
                     /gene="allS"
                     /locus_tag="JNZ05_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HAY8564280.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQHRPEISGFLEIFGNPRS"
     gene            49679..50161
                     /gene="allA"
                     /locus_tag="JNZ05_000962"
     CDS             49679..50161
                     /gene="allA"
                     /locus_tag="JNZ05_000962"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HAY8564281.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50239..51054
                     /gene="allR"
                     /locus_tag="JNZ05_000963"
     CDS             50239..51054
                     /gene="allR"
                     /locus_tag="JNZ05_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HAY8564282.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51144..52925
                     /gene="gcl"
                     /locus_tag="JNZ05_000964"
     CDS             51144..52925
                     /gene="gcl"
                     /locus_tag="JNZ05_000964"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HAY8564283.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGIINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52938..>53210
                     /locus_tag="JNZ05_000965"
     CDS             52938..>53210
                     /locus_tag="JNZ05_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HAY8564284.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12825 a        13704 c        14016 g        12665 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca acgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgac tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccggg aagggcagga tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttta tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatt
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctggc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aatcagcgag aatttgctgg cgtaatgtgt gctcttccgc tagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagct ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcat tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc cagccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaaccg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gtttttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttaaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagccgg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgcagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcgcagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacccgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgctactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctagaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ttcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcactg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggcaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg cggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaagc
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactct gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgttcgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cctggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggcgaag ctagcggcag aagccattga gcgcgacccg tggacggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggcgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca ggcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgac atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaacc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtattgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagccg gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctc atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc gccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactcctccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatca ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgccttcc atgcaaataa aatagcgtcg
    23641 ccgggctatc tggctgcgga caaaagagac gtgtttccac ctgcccatat tttgttggaa
    23701 ccatgtaagc tctggttgcc atttctggag cgcccgcatt ccagaatcgg cgctcaagcg
    23761 tgtaatactg tcgttgctca gcaatcgttc ccgttgcggg ccaggacggt aaatccggct
    23821 gaagagtatt cacaacggtc ttcatttcag cagaaataag gtccagaaca ggtagtttgt
    23881 tttccggctt catacaaaaa ctcctttcaa attacgtcat tgtaaggaac cactgccatg
    23941 aaaatgcgat cccgcctgct gatattgaaa ctggctgcgt ctcgcgcgct cccgtcagat
    24001 tgtgttaaca ttcgccgctc agttaaccac ccgtaaaaac aaccatgaaa tttcccggta
    24061 aacgtaaatc caaaaattac ttccctgtaa atgcacgcga tccgctgctt cagcaattcc
    24121 agccagaaaa cgaaaccagc gccgcctggg tcgtaggtat tgaccagacg ctggtcgata
    24181 ttgaagcgaa agtggatgat gaattcattg agcgttatgg attaagcgcc gggcattcac
    24241 tggtgattga ggatgacgta gccgaagtgc tttatcagga gctaaaacag aaaaacctga
    24301 ttacccatca gtttgcgggt ggcaccattg gtaacaccat gcacaactac tcggtgctcg
    24361 cggacgaccg ttcggtgctg ctgggcgtca tgtgcagcaa tattgaaatt ggcagttatg
    24421 cctatcgtta cctgtgtaac acttccagcc gtaccgatct taactatcta caaggcgtgg
    24481 atggtccgat tggtcgttgc tttacgctga ttggcgagtc cggggaacgt acctttgcta
    24541 tcagtccagg ccacatgaac cagctgcggg ctgaaagcat tccggaagat gtgattgccg
    24601 gagcctcggc actggttctc acctcatatc tggtgcgttg caagccgggt gaacccatgc
    24661 cggaagcaac catgaaagcc attgagtacg cgaagaaata taacgtaccg gtggtgctga
    24721 cgctgggcac caagtttgtc attgccgaga atccgcagtg gtggcagcaa ttcctcaaag
    24781 accacgtttc tatccttgcg atgaacgaag atgaagccga agcgttgacc ggagaaagcg
    24841 atccgttgtt ggcatctgac aaggcgctgg actgggtaga tctggtgctg tgcaccgccg
    24901 ggccaatcgg cttgtatatg gcgggcttta ccgaagacga agcgaaacgt aaaacccagc
    24961 atccgctgct gccgggcgct atagcggaat tcaaccagta tgagtttagc cgcgccatgc
    25021 gccacaagga ttgccagaat ccgctgcgtg tctattcgca cattgcgccg tacatgggcg
    25081 ggccggaaaa aatcatgaac actaatggag cgggggatgg cgcattggca gcgttgctgc
    25141 atgacattac cgccaacagc taccatcgta gcaacgtacc aaactccagc aaacataaat
    25201 tcacctggtt aacttattca tcgttagcgc aggtgtgtaa atatgctaac cgtgtgagct
    25261 atcaggtact gaaccagcat tcacctcgtt taacgcgcgg cttgccggag cgtgaagaca
    25321 gcctggaaga gtcttactgg gatcgttaag ttatcgtcgg ttcgtaggcc agataaggcg
    25381 ttcacgccgc atctggcatt tggctctcga tgcctgatgc gacgctggcg cgtcttatca
    25441 tgcctacata tttttcatat tttacatccg gcaaccaccg tttaccccgt caccacttca
    25501 cccgccggtg gcgtttccag cagttccagc atagtacggg caatttcacg ttcacccatc
    25561 actacctgat tcgcgccacg ttcggtgata tacgtcactt catcgtcata atgggcgcgg
    25621 gcgataatct caatatccgg gtttttcgcg cgggcagacg ccacaatctc acccgcttca
    25681 taaccgttgg ggatcgtcag gatcagccat tttgcacatt ccagatgtgc cagttgcata
    25741 atttcttcgt tcgctgcatt gcccaataca gcgcggaccc cacgctctcg cagctcatca
    25801 acacgggttc gtgacgtctc aatcaccacc agcggaatat cagaggcgag caatttctcc
    25861 cccagcaggc tgcctacacg accgtaaccc accaatagcg catggttgca aatatccact
    25921 gggatctgtt tctcttcttc gattgcctct tccagcgtct gctcttccag cgtttcggtc
    25981 ttcgccagat atttctccag tagtgcgaac agtaccgggt tgagcataat cgacaggatc
    26041 gcccctgcca gtaccaggtt ttgtccggcc tgcggcagta aattcaatgc cattcccagt
    26101 cccgccagga taaacgcgaa ctcaccaatc tgcgccaggc tggcggcgat ggttaatgcc
    26161 gtacgttggg agtgaccaaa cagacggaca aggaacaggg ccgcgaccga tttaccaaac
    26221 agaataattg ccagcgtcgc cagcactgcc agcggttgct gaatcagaat taacggatca
    26281 aacaacatcc cgactgagac aaaaaacagc accgcaaacg cgtcgcgcaa tggcaacgta
    26341 tcgtgggcgg cacggtgact caattcagac tcgttcagta ccatcccggc aaagaacgca
    26401 ccgagtgcaa aggagacatc aaacagctct accgcaccaa aggcaatccc taacgccagc
    26461 gccagcactg acagggtaaa cagctcgcga gaaccggttg ccgcgctgcg tgccataatc
    26521 cacggcacca gacggcgacc taccagcatc ataatggcga taaatgcgat caccttgccg
    26581 atggtgatcc ccatatcgac tgcaagagtg gcaaagccca catcgccctg ttccatcatt
    26641 cctgccactg cgggcagcaa caccagcgtc agaaccatca ccaggtcttc cacaatcaac
    26701 caaccgatgg cgatttgccc acgctgactg tctattaatt gccgttcttc aagtgcgcgc
    26761 agtaacacca cggtactggc ggtggaaaga cataagccga acacgatacc ggtcattaac
    26821 gaccagccca gcacggcgga gagcgccata cccagcagcg tcgccacgcc tatctgggcg
    26881 atcgcgccgg gaatggcgat ggactttacc gccatcaaat ccttgagcga aaaatgcaaa
    26941 ccgacgccga acatcagcaa aataacgccc agttcagcca gttccggcgc aagcttggta
    27001 tcggcaacaa agcccggagt gaatggtcct gccagcacac ccgctaacag atatcccacc
    27061 agaggagaaa tacgtagttt attggccagc atgccgagga taaaggcgag cacaaggccg
    27121 ccaacaatgg tggtgataag cggggtggcg tgatgcattc cgtctccttt tcctggtggt
    27181 tattgtccat ttttggccgg gaaaaccaaa attacaggta atagtttatg acaatttcat
    27241 tgatgatgtt tatgaataat tgttgaattt tgcagaaaaa tggaattagc tgcaaaaaaa
    27301 gcacggatcg gaaaacagaa ggcggtttaa cagaggaaag gcttatggcg actgtaaaac
    27361 gatgcagcca aagtttgcct ttggctgcaa tgaaatcagt ctttatgccg gttatcaggc
    27421 aggaatatgg tcaacatccc caatagtggc aggaaagcac agattttata gactaactcg
    27481 atgctggtgt gatcggcgat aagccccaga actgccgctc ccagacctcc catgccgaaa
    27541 gcaaaaccga aaaagagtcc agaaatcata ccgatacgtc ccggaagtag ctcctgagcg
    27601 tagaccagaa tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc
    27661 cccgtccagt gcaggcgggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata
    27721 gagccccaaa tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta
    27781 cctgccgcaa cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat
    27841 ccgaattttt gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat
    27901 ttcgagaaaa tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt
    27961 ggattgatta tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa
    28021 cggctaattt gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg
    28081 ttgcctttgc cataaggcgc gataatcacc gccgccagca aggggcccag ggaactgcca
    28141 aagttgccgc cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata
    28201 cgggctacgc gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgcc
    28261 gccagcagaa ctgcgccaaa actgcccggc agcgcaagca gcaccagacc acttaaggta
    28321 aagcacatgc caattggcaa cgaccacggc atcggatatt tatcggtcca gtagccgacc
    28381 actggttgca gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata
    28441 aatgtcagag aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc
    28501 atgtcgttca gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc
    28561 ttggtcgttg acgcagccgc gcccgccaca ggctgggttt gttcactcat tgccatagga
    28621 aagtcacttt ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact
    28681 aacatacctg tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct
    28741 ggatgcagat ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt
    28801 ttaacacgtt gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg
    28861 ttagcgctgt taaccacatt tacactggcg agtgaaactg ctctggcgta tgagcaggat
    28921 aaaacctaca aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat
    28981 gaatatggcg aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag
    29041 gttgcggctg aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg
    29101 cccgagtctg acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat
    29161 gacgcgatgg cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag
    29221 gaaaagtggg ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag
    29281 cgcctgttta aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg
    29341 ctgacaaccg atgacacagc aaaaattggt aacccggaat acttcactga tatcgaattt
    29401 cgtaagcccg ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca
    29461 gacattatta tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac
    29521 gcaccgggcg atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc
    29581 ggtggtcact cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac
    29641 gtgccgggta cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat
    29701 gagtggggca aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg
    29761 gttaactacc agctgattcc ggtgaacctg aagaagaaag tgacctggga agacgggaaa
    29821 agcgagcgcg tgctttacac tcctgaaatc gctgaaaacc agcaaatgat ctcgctgtta
    29881 tcaccgttcc agaacaaagg caaagcgcag ctggaagtga aaataggcaa aaccaatggt
    29941 cgtctggaag gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttgatt
    30001 ctggcagccc aaatggatcg cactggtgcc gactttgcgg tgatgagcgg aggcggaatt
    30061 cgtgattcta tcgaagcagg cgatatcagc tataaaaacg tgctgaaagt gcagccattc
    30121 ggcaatgtgg tggtgtatgc cgacatgacc ggtaaagagg tgattgatta cctgaccgcc
    30181 gtcgcgcaga tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg
    30241 gcgaaagacg gcaaactgaa cgaccttaaa atcaaaggcg aaccggtcga tccggcgaaa
    30301 acttaccgta tggcgacatt aaacttcaat gccaccggcg gtgatggata tccgcgcctt
    30361 gataacaaac cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat
    30421 atccagaaaa gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg
    30481 cagtaatccg aaagtgccgg atgtttgcat ccggcacaat gcttaatcgc ggcgggcgat
    30541 atcagcaaat ttggcatcga ggatctttgc cagatcgcct gccgccagtt cgatatccag
    30601 tccgcgcttg ccgccggaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat
    30661 cgttggcaga cgttttttct gccccagtgg gctaatcccc ccaaccaggt atcccgtcga
    30721 acgctgcgcg accatcggat cggccatctc aactttcttg gcacccagcg cttttgctac
    30781 ttttttaaga tccagttgac ctgcgaccgg cgtaacggcc acggcaaggt gtttcatatc
    30841 accgttcact gccaccagca gcgttttgta gacctgatcc ggattcaaac ctaatttttt
    30901 gacgacttca tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa
    30961 cgaaatcttg tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta
    31021 aacaaaaaaa cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc
    31081 taagagtatt ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt
    31141 gacggcataa taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca
    31201 tttttttagc gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc
    31261 gcgaccacat atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc
    31321 tgatgcatca gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta
    31381 ttttgcggcg gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta
    31441 tgccagtggg tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac
    31501 atggcaatct ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc
    31561 gcctgcaata gctggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc
    31621 gtcgcctgaa gaaccatcgc gatttgccac agcggttggg tagaaaagag tgagggagaa
    31681 atgcccatct cctggctaat gtgctgcagg ttttggagtt gctcctcgct aatgcgctct
    31741 tccagcgcct cgcaggcagg caaattagca aaaggcgtat cgctggtgga aacatccgcc
    31801 tcgacgagca gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc
    31861 atatcgtggc tacccatatg aatactgcca atcagatgaa aatggcgatt gccggggagg
    31921 gtgatatcga tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt
    31981 acccggtaca acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga
    32041 gggatggcgc aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct
    32101 tgcatcgcta ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc
    32161 gactacaccc ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa
    32221 tgagttagca gagttgctgc atgttcatcg taatcgcgtc agtgcactga tcaataacaa
    32281 tcgtaaactc accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga
    32341 ttttaggcta aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac
    32401 ccaggaagaa ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg
    32461 tgcaaaaaag gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt
    32521 cacgccgcat ccggcatccg agcaccattg cctgatgcga cgcttacgcg tcttatcagg
    32581 cctacaaacc tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcagtc
    32641 atgcgtcgat gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg tacttattcc
    32701 ttcggtttaa accgcagcaa ccggttggcg ttactcacta cggtaatcga cgagagcgcc
    32761 attgccgctc cggcaactac cgggttaagc agtgttccag taaacggcca caaaataccg
    32821 gcggcgaccg gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg
    32881 ttgcgcagcg ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc
    32941 atcagggtaa tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg
    33001 acatccgcct gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga
    33061 cgtccttcac tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca
    33121 atcacttcat caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg
    33181 gtcaacatca ccagacgata tcccgcttta tgcaggcgtt gcagcgccgc cacgctatca
    33241 ctacgcaacg gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc
    33301 acaggcgttg ctccttgcga tgcctgagct gaaatatcgg cttcaatcgc tttagtatcg
    33361 acttgctgat catttaacaa cgcctgatta cccagcaata acgcatgacc ttctgcttca
    33421 ccgctcacgc ccagcccgcg caatgtgcgg aaaccgttga cctgcggtag ctgcatatcg
    33481 cctgctttat caaggatcgc tcgtgccaat ggatggctgg aaccttgctc cagcgccgcc
    33541 gccagacgca gcgcctgcgc ttcgtcaaca tcagcaaatg ttttcactgc gacaacctgc
    33601 ggcttccctt cggtcagcgt cccggtttta tcgaacacga cggtatcgag tgtactggcg
    33661 cgttgcagcg cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg
    33721 gaaataatcg acatcggcgt cgccagtccc agcgcacacg ggcaggcgat aatcagcacc
    33781 gtagtggcaa tcaccagggt atagacaatc tgcggtgccg gaccaaagaa ataccagatt
    33841 gccgcactga caagcgcaat aaccactact accggcacaa atacggctga gattttatcc
    33901 gccagctgac cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatcctt
    33961 gatagcgtgg tatggctgcc aaccgcactg gcacgaaaca gcacactgcc gtcctgtacc
    34021 actgtcccgg catggacgct atcgccttcg cctttttgct gcgggattgg ttcgcccgtc
    34081 agcatcgctt catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga
    34141 tcgccggtcg tcaggcgcag caacatacct ggctgcactt ctgccagagg cacgcttttt
    34201 tcaccttcgt cagtaaccag gcgcgccgtc ggtggggtta aatcgagtaa tttctccagt
    34261 gccttagaag aacgctgacg tgcgcgtgct tccagcatat ggccgagatt gatcagaccg
    34321 ataatcatcg cactggcttc atagtaaaga tgtcgcgctt ccatcgggaa ccactgcggc
    34381 cacaggttga cgctcatcga atagagccac gccacgcccg ttcccagcgc caccagcgta
    34441 tccatcgtcg ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgccg
    34501 gcgaaaacca tcaccgccag ggttatcagg ccgataacca accacaggct gcggttgtcg
    34561 gcggtgacca tcatgttatc gccgatcatc ccccagacca tcaccgggat acccaccgcc
    34621 agtgcgacaa ttgcctgcca gcggaagcgc ttcatcgtag cgacggcggt ttcttgctgg
    34681 cgttcgcggc gtttcgcgtc atcttcaatc gcttccgcgc cgtagcccgc tttttccacc
    34741 gcctgcacta aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg
    34801 tttacccgtg cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca
    34861 cagctggcgc agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc
    34921 gctgccggaa gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc
    34981 agcggtttag cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg
    35041 atcagctgtt ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgct
    35101 tgttccacat ccgggcgctg ttcaagactt tctttcacgc gtttaacgca gtgaccgcag
    35161 gacaggccgt ccaggttcag gtcgatagtt tgtgacataa aacactcctt taagacagtt
    35221 ttgactggct gtgataaagg ttaaaccttc cagaaagggg aaggtcaaga aattaataaa
    35281 ccaggcgggt aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac
    35341 atttatttct ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcctg
    35401 aaaatcggca caggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga
    35461 tgttagatgc aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac
    35521 ttaacggcgg acaaaatgcc gattacattc cctttctggc gaatgtacca ggtcaactgg
    35581 cggcagtggc tatcgtgacc tgcgatggca acgtctatag tgcgggtgac agtgattacc
    35641 gctttgcact ggaatccatc tctaaagtct gtacgttagc ccttgcgtta gaagatgtcg
    35701 gcccgcaggc ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag
    35761 ttatcgcctt agagttgcat ggcggcaaac cgctttcgcc actggtaaat gctggcgcta
    35821 ttgccaccac cagcctgatt aacgctgaaa acgccgaaca acgctggcag cgaattttac
    35881 atatccaaca gcaactggct ggtgagcagg tagcgctctc tgacgaagtc aaccagtcgg
    35941 aacaaacaac caacttccat aaccgggcca ttgcctggct gctgtactcc gccggatatc
    36001 tctattgtga tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct acgctcatca
    36061 atactgttga actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgttga
    36121 cgcataaacg cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg
    36181 aagggctgta tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg
    36241 gtgtaggtgg cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac
    36301 caccgctgga cgaagaaggc aatagtgttc gcggtcaaaa aatggtggca tcggtcgcta
    36361 agcaactcgg ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg
    36421 taacaaaccc ctcggtctat ggaacgtcgt ttccatcggt attggggcaa tggtgggggc
    36481 ggggatcttc gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctggggcgc
    36541 ctttgctttt ggcggtattg tggcgatgtt ttccggttat gcctatgcgc gtctgggggc
    36601 gagctatccc agcaatggcg gtattatcga cttttttcgt cgcggattag gcaacggcgt
    36661 cttttcgcta gcgctctcgt tgctatacct gttgacgctg gcggtgagca tcgccatggt
    36721 tgcccgtgct tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca
    36781 ccttattttg ctctacgcgt tggggatcat tgcggtgatg acgcttttca actccttaag
    36841 caaccatgcg gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt
    36901 attgctgatt attgccggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc
    36961 ccccagctcc ggtgcgttct tctcctgtat tgggataacc ttccttgcct atgcgggctt
    37021 tggcatgatg gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc
    37081 gtttctggtg gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct
    37141 tagcgatgta tcggcattag agttagaaaa atatgctgat accgccgtag cgcaggctgc
    37201 ttctccgctg ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac
    37261 ggcttcaggc attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag
    37321 cgaacgcgaa ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa
    37381 catcatcgtc gtggtgttga taatgctgat gacggcggcg ctaaatttag actcactcgc
    37441 cagcgttgcc agcgccacct ttttgatctg ctatctggcg gtgtttgtgg tggcgatccg
    37501 cctgcgtcat gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt
    37561 gttggtgatc gttggcttta tctacagcct gtggtcccag ggcagccgtg cgttgatatg
    37621 gattattggt gcaatcttac tcagccttat tgtggcaatg gtcatgaagc gtaataaaac
    37681 cgtataacac ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg
    37741 cactggcaaa aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc acaggaggcg
    37801 ttgcgcgaac gatgaacatc agcgatgtag caaaaattac cggcctgacc agcaaagcca
    37861 ttcgcttcta tgaagagaag gggctgatga cgccgccgat gcgcagcgaa aacggttatc
    37921 gcacctacac gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg
    37981 gctttaacct ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca
    38041 gcgccgacgt caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg
    38101 agctgcaatc catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca
    38161 gcgccgactg cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggtgat
    38221 taagacgaga cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatac
    38281 gtacctgcac cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata
    38341 tgaccgcgcc cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc
    38401 ccgcgctggt ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat
    38461 ttccaccaca gccaggcggc gagcagcgtc aggatggcaa acattacccc ttgccactcc
    38521 caacccagcg gcaccagcca gaccaccagg ccggtaatca ccgctgccac gccgctccac
    38581 aacaaataac catttccgcc cagcatctcg gctgccagca gcaacccgcc gagactgagc
    38641 cagaaaatat gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc
    38701 tgtctttcac cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg
    38761 gcatcatcac tactttgctg ttactggagg aaccgatctg ctgtaacgct tcggtgtatt
    38821 tttgcgctac gaagtagttc accgcctgaa tatcaccgga ggcgatagct tcagacacca
    38881 ttttggtggc gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc tgtaaaaacg
    38941 ccgactgacg ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga
    39001 ggatttccgc ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg
    39061 ctttcatctg cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt
    39121 caatacgggt gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc
    39181 gtgagttgat gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg
    39241 tacggatgtt agtcatggtc aggttgatga tcgccagctc cagattgctg acttcatagg
    39301 cagcgcgtgg cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt
    39361 tatctttcga gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct
    39421 tgcgaccaat gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg
    39481 tatagcgacc aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg
    39541 cgccgacaat gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa
    39601 cctcctgttg taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg
    39661 attactgaaa gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc
    39721 agaaaaaggg aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat
    39781 atctggcggg tgatacgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat
    39841 ttaagttaat taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt
    39901 cattgatcag cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa
    39961 aaccagaaat ctaccgccag caagtctctt actgcgccca gacgccgacg ctgtttggcg
    40021 atacggtata cgataatctg atctttccct ggcagatccg tgaccagcag cctgacccgg
    40081 ccatttttct cgattttctc gaacgcttcg ctttgccgga cagcattttg acgaagaata
    40141 tcgccgagct atctggtggt gaaaaacaac gcatctcatt gattcgtaac ctgcaattta
    40201 tgccgaaggt tttattgctg gatgaaataa ccagtgcgct ggatgaaagt aataaacata
    40261 acgtcaatga gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga
    40321 cacacgataa agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg
    40381 gagaaatgca ggaagtacgc tatgaactcg cataatatta ctaacgaatc attagcactg
    40441 gcattaatgc tggtggtggt ggcaatctta attagccata aagaaaaact ggcgctggag
    40501 aaagatattc tctggagcgt cgggcgagcg ataattcagc tgattattgt cggctatgtg
    40561 ctgaagtata ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc
    40621 tgctttaatg cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc
    40681 tcatcgttta ttgccattac ggtcggggcg ggaattaccc tggcggtgct gattctctca
    40741 gggtcgattg aatttatccc gatgcaggtg atccctgtcg ccgggatgat tgccggtaac
    40801 gccatggtag cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag
    40861 caacagattc aggagaaact gagtcttggc gcgacgccga agcaggcttc agcgatattg
    40921 attcgcgaca gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc
    40981 ttagtgagtt taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag
    41041 gcgattaaat atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc
    41101 attattgcct gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg
    41161 actcaattga agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg
    41221 ttcatgtgcc gaacaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc
    41281 gacgccagtg catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc
    41341 tgaccgtctg cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct
    41401 tcattgcgcc caacctgatg cagttgcagc gccagttgcg tcgccagtgc ggcatcttct
    41461 ggattctccg ccacctgctg ttgcaactgt tgaatttccg gcttatcagc cgcctgcttc
    41521 agcagttcga tttgcgctac cagcccctgg tagcgggtgt cctgatcctg caacggaatg
    41581 gttttcagca ccgcttccgc atcttcagaa cggttcagcg caatcagcgt ttctgccagc
    41641 agcaggccga tctccccgtt ctgattcgac aactgccagg cgtctttcag caatggcagg
    41701 gcatcggtgt aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct
    41761 tcgcgcggca gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg
    41821 aagccatcta ccggttgccc gttctggaac agatacacgg tcggaatcgc gcgcagacca
    41881 aactgggcgg caatcatctg ctccgcgtcg cagtccagct tcgccagaat aaattgcccg
    41941 ttgtactgcg ccgcgaggct ttccaggatc ggggttaact gcaaacagtg ctggctacgt
    42001 tcagaccaaa aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg
    42061 ttagattcgt taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat
    42121 attttctttg acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc
    42181 atcacgcgcc caggcagcag gcgctcgagc accattactg cccaggtcac cagcgttacc
    42241 ggatagcgca tcttcggctt ctcgctaata aaagcatggc gcactttgtc gaccaccgct
    42301 tccggtccca acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc
    42361 gtctggttga cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact
    42421 ttaattccgc tgtggcgtag ctccatgcgc agcgcgtctg accacgcctc cagcgcatat
    42481 ttactggccg cgtaagcgcc acgacccggc gtggagatta atcccatcac cgatgatgtc
    42541 atcacaatac gcccttcacc gtgcggtaac atcgcgggta acaggcgcat ggtgagctgg
    42601 tgtgcgccga aaaagttggc ggaaaactgc tgttccatct gcgcacggct gatggtggaa
    42661 agggggccat acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc
    42721 agggcgatca cctcgtctgc tgcgcgatta acactttctg gtgaatccag atcgatcaac
    42781 acgccggtaa atcccatgtt gttcatacgc tcaacatcat tcggttttcg gcaacctgcc
    42841 agcacatgaa aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa
    42901 catccggtaa ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta
    42961 tgagtcatga tttactaaag gctacaactg cttcgccatc cagtcggcaa taaacggctg
    43021 ggcgtcgcgg ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc
    43081 ttccataaaa aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc
    43141 gctaaaggct tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa
    43201 tggttcagcg ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg
    43261 ctgtggctga aaaccacgca aaccatcatt gcgcccagtt caaccagcac ccaacgcggc
    43321 tgatgctgtt tgagcagagc cggaaggcgc gccagccctt gttgcgaggt gtcgccgctg
    43381 atgctggcat taactaccga cgttttactc tgccacttat cattcaacaa ggcaggccag
    43441 gccgcgctgg cagacattcg atatccggcg ctcaggctat cacccagaat caataacgtg
    43501 tccgctgcgg cggcacggaa ggttaacagg accaggaaca ggaagggcaa atgccagcgg
    43561 aaaacattgt tgaagttcat catcttaaga agtccgtcgg tcagggggag catgaactct
    43621 ccatcctcac cggagttgag ctggttgtca aacgtggcga gaccatcgca ctggtgggcg
    43681 agtcgggatc gggtaagtca accttgctgg cgatcctcgc cgggcttgat gacggcagca
    43741 gtggcgaagt gagtctggtg ggacaaccgc tacataatat ggacgaagaa gcgcgggcaa
    43801 agttgcgcgc gaagcacgtc ggctttgttt ttcagtcatt tatgttaatt cctaccctta
    43861 acgcgctgga aaacgtcgag cttccggctc tgctgcgcgg tgagagtagc gcggaaagtc
    43921 gtaacggggc gaaagcgttg ctcgaacagt tagggctggg taaacgtctg gatcatcttc
    43981 cggcacagct ttccggcggt gaacagcaac gagtggcgct ggcacgagcc tttaatggtc
    44041 gacctgatgt gctgtttgcc gacgaaccca ccggcaacct tgaccgccag acgggcgata
    44101 aaattgccga cctgctgttt tccctcaacc gtgaacatgg caccacgttg attatggtga
    44161 cccacgacct gcaactggcg gcacgctgcg accgctgctt acggctggtg aacgggcagt
    44221 tgcaggagga agcatgattg cacgttggtt ctggcgcgaa tggcgttcgc cgtcgctatt
    44281 aattgtctgg ctggcgctaa gcctggcggt ggcctgcgtg ctggcgctgg gcaatatcag
    44341 cgatcgcatg gagaagggct taagccagca aagccgtgag tttatggcgg gcgatcgggc
    44401 gttgcgcagt tcacgcgaag tgccgcaagc gtggctggag gaagcgcaaa agcgcggcct
    44461 gaaagtcggc aagcagctga ctttcgccac aatgaccttt gcaggcgaca caccgcagct
    44521 ggcgaacgtc aaagcggtgg atgatatcta cccgatgtat ggcgatctgc aaactaatcc
    44581 ccctggcctg aaaccgcagg cgggcagcgt attgctggcc ccacgcctga tggcactgct
    44641 taacctgaaa acggacgaca ccattgacgt gggcgatgcc accttgcgga ttgccggaga
    44701 agtgattcag gaaccggatt ccggttttaa ccccttccag atggctccgc gtctgatgat
    44761 gaatctggcg gatgtcgata aaaccggagc cgtgcagccg gggagtcggg tcacctggcg
    44821 ttataaattc ggcggcaacg agaaccagct cgacggctat gagaaatggt tgttacctca
    44881 gcttaaaccc gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc tggggcgatc
    44941 gatggaacgc tcgcaacagt tcctgctgct ttcggcgctt ctgaccttgc tgctggcagt
    45001 ggcagcggtg gcggtagcga tgaatcatta ctgtcgcagt cgctacgatc tggtggcgat
    45061 cctcaaaacg ctgggggcag ggcgagcgca actgcgtaag ctaatcgtcg gtcagtggtt
    45121 gatggtgctg acgctttcag ccgttaccgg tggggccata ggcctgttgt tcgaaaacgt
    45181 gttgatggtg ctgctcaagc ccgttctgcc tgctgcacta ccgccagcca gcctctggcc
    45241 gtggctgtgg gcgcttggca ccatgacggt catctcgctg ctggtggggc tacgaccata
    45301 ccgcttgttg ctggcaacgc agcctttacg cgtattacgt aatgatgtgg tcgcgaacgt
    45361 ctggccgctg aagttttatc tgccgattgt cagtgtggtg gttgtgctgc tgctcgccgg
    45421 attaatgggt ggcagcatgc tgctttgggc ggtgctggcg ggcgcggtag tactggcttt
    45481 gctgtgcggt gtgctgggct ggatgctgct gaatgtactt cgccgcatga cgctgaaatc
    45541 gctgcctctg cgcctggcgg ttagccgcct gttacgtcag ccgtggtcaa cgttaagcca
    45601 gctttcggca ttttcgctct cctttatgct gctggcactg ctgctggtgc tgcgtggcga
    45661 tctgctcgac cgctggcagc agcagctacc gccagaaagc ccgaactact ttttaattaa
    45721 catcgccaca gaacaggtag caccgctaaa agcgttcctc gcggaacatc atatagtccc
    45781 ggaatcattt tatccggtag tgcgggcgcg gctgacggcg attaacgata agccgacaga
    45841 aggtaatgaa gatgaggcgc ttaatcgcga actcaatctt acctggcaaa atacgcggcc
    45901 cgatcataat ccgattgtcg ccggtaactg gccgccaaaa gccgatgaag tgtcgatgga
    45961 agaggggctg gcgaaacgct taaacgttgc cctcggtgat accgtgactt ttatgggcga
    46021 tacccaggag ttccgcgcta aagtgaccag cctgcgcaaa gtggactggg aaagtctgcg
    46081 gcctaatttc tattttattt tccctgaagg ggcattagac gggcaaccgc agagctggct
    46141 taccagtttc cgctgggaga atggcaacgg catgttgacg caactcaacc gccagttccc
    46201 gaccattagc ctgttagaca ttggcgcgat tttaaaacag gtcggtcagg tgctggagca
    46261 ggtaagtcgg gcgctggagg tgatggtggt gctggtcacc gcctgcggta tgttgctgtt
    46321 gctggcacag gtgcaggtgg gaatgcgcca gcgtcatcag gagctggtgg tatggcgcac
    46381 actcggtgcg gggaaaaaac tgctgcgcac gacattgtgg tgtgagttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tgtaactatg attttcttca tgtgctagat gaggactgcc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aacaaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg ttttttatat ctgtattctg gtgcaaccat tttgttctca cgacaatcgc
    47341 cgtttcagaa taccgcccat cactgcgcct taacccatac cgccacttcc gcccactcac
    47401 cgcgaaagac aactttttcc gcttttttct caagctgata gcgatacatt gggtcgtaat
    47461 attctttaag taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt
    47521 gcgttgtcag cgccgtatcc agctgggcag cgagttcgtt atagcgctgt aaccccagcc
    47581 gacgcttaat cgccgaaagt ccgtgatgca ggtattcgca atattcctgc cagccctgtt
    47641 cgtcgccata agcgtgggta aaatcatgat gcatacgaaa gaaatactct tcattcaggc
    47701 gttcaagacg gatctcaaac ggatcttcta ccaccgcaat cgtcgcctga gtcattcgct
    47761 cgcgcagaca ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc
    47821 acaggcgcaa atcctgacgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa
    47881 aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa cgccgaaccg cgatgacgcg
    47941 ccagcccttc cagatcaaca ccgtttggct gttgttgcac taatagcgtt ttaccgctgc
    48001 cggtacaacc gccaatcagc actatcggtt tttgtgccag ttcaatggtc gcctgaatcg
    48061 cggtctggcg cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcttcat
    48121 gcagccagcg ttgcacaata tgtgaacgtt gaccgccacg ggcgcagcag agaatacctt
    48181 gcggattttg caggcacgct gcccgccagg cgtacatacg ctgctgacga atttcacccg
    48241 ccaccagttt atgccccagc gccagcgctg cgtctgagcc ttgctgttta tagcaggtgc
    48301 caacggcggc gcgttcatcg ttattcatta acggcagatt gatagcggcg ggcattgcgc
    48361 cttgctcaaa ctcgataggg gcgcgaacat caataatggg cgtatcagca atcagcaggg
    48421 cacgatagtc ctgttccgtg tgtctctctt gcatagttaa aagtgaacct caaatcagct
    48481 tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtgggttgcc
    48541 gaaaatttct aaaaatccgc tgatttccgg cctgtgttgg gtaaacaagg tcacaatatc
    48601 ttctaccgct ttgccgctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg
    48661 catcgttggg attacccggc tgaccagttg ttgattatcg atcattgact ggcaaagtga
    48721 ttttggcaaa aaaccaatgc caacacccgc cagatgggcg gcgattttcg tttccatatc
    48781 gggaacaata atctcttttt gccccggcaa tcgccaggcg acgcgtttgg ttaaggtgcg
    48841 ggcgctgtct tcaatattga ccgccggaaa gcgacgcaac tgcgcttctg ttagcggctc
    48901 ttcaacgttc gccagcggat gatccgccgc catgacaaat cgccattgca ccgatcctaa
    48961 gggatcaaga ctaaaggtat ttgccagcgc ctcagttccc gtgaccccga tagccagcga
    49021 aaaaccttcg tacaatagcg agtcccagac gcccatatag atttgtcggg agatgtgaaa
    49081 ctgggtaaag gggtaacgct cattcagcca cgccagcaac tgggcgacgg cctgggggtt
    49141 atagagcagg ttgttgatga caatattcac ctggcgttcc acgccatcat tcacctgttg
    49201 cagctcgctg ggcatacttt ccagccagct cagccagtct ctggcctggg aaagtagatg
    49261 ctcgccagcc gctgtcaacg ttacgctacg agtcgtacgg aaaaacagcg ctactccggt
    49321 attctcttcc agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc
    49381 tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg
    49441 atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct
    49501 gatttatcag tatttttaca tgataatcct gttcaatttg tggactaaat ctagttttgg
    49561 aaaaacattc caacttttgt attgatgttg ttctcttaag gttttagatt gcctgttatt
    49621 gaaaccaagc tgaccggtcg gcggtggttg aacggaatta tgttacaagg acaaaaagat
    49681 gaaacttcag gtattaccgt taagtcagga agcctttagt gcttatggcg acgtaatcga
    49741 aacgcagcaa cgggattttt tccatattaa caatggcctg gtggagcgtt accacgattt
    49801 ggcgctggtt gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc
    49861 ggcgaatctg ccgctgacca ttcacgaact cgaacgtcat ccgctgggca ctcaggcctt
    49921 tatcccaatg aaaggtgaag tgtttgtggt ggtcgtggcg ttaggcgacg acaaaccaga
    49981 cctgtcaacg ctgcgggcgt ttatcaccaa cggtgaacag ggggtgaatt accatcgtaa
    50041 cgtctggcat cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg
    50101 cggcggcagt gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg
    50161 acgcctgcaa ccgacttgca taagataaac taattgttca ttgtttatgc tcacttgtag
    50221 gtcggagtta acgtaggtat gacggaagtt agacggcgcg gtaggccagg acaggcggag
    50281 cctgtggcac agaagggcgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg
    50341 gaaaaaagtg ggggaagttc gtcggttagc gatatttctc tcaatctgga tttgccgctc
    50401 tccacgacct ttcgcttgct gaaggtttta caagcagcgg attttgtcta tcaggacagt
    50461 caattaggct ggtggcatat aggattaggt gtctttaacg tcggtgcggc gtacatccat
    50521 aaccgcgatg tcctctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc
    50581 gaaacggtca atgtcgcgat ccgtaacggc aatgaagcgg tattaattgg tcagttagag
    50641 tgtaaatcga tggtcaggat gtgtgcgcca ctgggcagtc gtctgccgct gcatgcttcc
    50701 ggtgcgggca aagcgctgct ttatccgctg gcggaagagg agttgatgag catcattctg
    50761 caaaccggtt tgcagcagtt tacgccaact acgcttgtgg atatgcccac cttgctgaag
    50821 gacctggaac aagcgcgtga actgggctat accgtagata aagaagagca tgttgtaggt
    50881 ctgaattgca tagcttcagc aatttacgat gatgtcggta gcgttgttgc cgctatctcc
    50941 atctccgggc cttcatcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc
    51001 agagacaccg cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg
    51061 tcgcatcccg ctctgcggag cgggtttttt tgacaaaatt taaaagttgg aaaatttttc
    51121 caattaatag aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg
    51181 tgctggagaa agaaggtatc accaccgcct tcggcgttcc gggagctgca attaatccgt
    51241 tctactcagc gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag
    51301 gtgtttcgca catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc
    51361 tggggacttc cggtcctgcg ggcacggaca tgatcaccgc gctctattcc gcttctgctg
    51421 attccattcc tattctgtgc attaccggcc aggcaccgcg cgcccgtctg cataaagaag
    51481 attttcaggc cgtagatatt gaagcaattg ctaaaccggt cagcaaaatg gcggttacag
    51541 ttcgtgaagc ggcgctggtg cctcgcgtgc tgcaacaggc atttcacctg atgcgttctg
    51601 gtcgtccggg tccggtactg gtggatttac cgttcgacgt tcaggttgcg gaaatcgagt
    51661 ttgatcctga catgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga
    51721 tcgaaaaagc tgtagaaatg ttaatccagg ccgaacgtcc ggtgattgtt gccgggggcg
    51781 ggataatcaa tgctgatgca gctgcactgt tacaacagtt tgctgaactg accagcgttc
    51841 cggtgatccc aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg
    51901 ggatggtcgg tctgcaaacc gcgcatcgtt acggtaacgc aacgttgctg gcgtccgaca
    51961 tggtgtttgg tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca
    52021 ccgaagggcg caaaatcgtt catattgata ttgagccgac gcaaattggt cgcgtgctgt
    52081 gtccggatct cggcattgtc tctgatgcta aagcggcgct gacactgctg gttgaagtgg
    52141 cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa agaatgggtc gccgactgcc
    52201 agcagcgtaa acgcactttg ctgcgcaaaa cccacttcga caacgtgccg gtgaaaccgc
    52261 agcgcgtgta tgaagagatg aacaaagcct ttggtcgcga tgtttgttat gtcaccacta
    52321 ttggtctgtc acaaatcgct gcggcacaaa tgctgcatgt ctttaaagac cgccactgga
    52381 tcaactgtgg tcaggctggt ccgttaggct ggacgattcc ggcggcgctg ggggtttgtg
    52441 ccgctgatcc gaaacgcaat gtggtggcga tttctggcga ctttgacttc cagttcctga
    52501 ttgaagagtt agccgttggc gcgcagttca acattccgta catccatgtg ctggtgaata
    52561 acgcctatct cggcctgatt cgtcagtcac aacgcgcttt tgacatggac tactgcgtgc
    52621 aactcgcttt cgagaatatc aactccagtg aagtgaatgg ctacggtgtt gaccacgtaa
    52681 aagtagcgga aggtttaggt tgtaaagcta ttcgggtctt caaaccggaa gatattgcgc
    52741 cagcctttga acaggcgaaa gccttaatgg cgcaatatcg ggtaccggta gtcgtggaag
    52801 ttattctcga gcgtgtgacc aatatttcga tgggcagcga actggataac gtcatggaat
    52861 ttgaagatat cgccgataac gcagcggatg caccgactga aacctgcttc atgcactatg
    52921 aataagggag ataaatgatg ttacgtttct ctgctaattt atcgatgtta tttggtgaat
    52981 atgattttct cgcccgtttt gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta
    53041 tgtttcctta tgactacgac attgaagaat taaaacaggt actggcgagt aataaactcg
    53101 aacatacgct gcacaattta ccggcaggtg actgggcggc gggcgagcgc ggtattgcct
    53161 gtattcctgg ccgtgaagaa gagtttcggg atggcgtagc agcagcgatt
//
LOCUS       DADMEQ010000016        52549 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.016, whole genome shotgun sequence.
ACCESSION   DADMEQ010000016 DADMEQ010000000
VERSION     DADMEQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.016"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..354
                     /locus_tag="JNZ05_000966"
     CDS             <1..354
                     /locus_tag="JNZ05_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564285.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="JNZ05_000967"
     CDS             complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="JNZ05_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HAY8564286.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="JNZ05_000968"
     CDS             complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="JNZ05_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HAY8564287.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLPFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFSNAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3052..3261
                     /gene="mokC"
                     /locus_tag="JNZ05_000969"
     CDS             3052..3261
                     /gene="mokC"
                     /locus_tag="JNZ05_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HAY8564288.1"
                     /translation="MLNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVT
                     RKDLCEVHIRTGQTEVAVFTAYESE"
     gene            complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="JNZ05_000970"
     CDS             complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="JNZ05_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAY8564289.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="JNZ05_000971"
     CDS             complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="JNZ05_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAY8564290.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRATD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSSRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6877..7281
                     /locus_tag="JNZ05_000972"
     CDS             6877..7281
                     /locus_tag="JNZ05_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HAY8564291.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7307..8020
                     /gene="msyB"
                     /locus_tag="JNZ05_000973"
     CDS             7307..8020
                     /gene="msyB"
                     /locus_tag="JNZ05_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HAY8564292.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8169..8735
                     /gene="satP"
                     /locus_tag="JNZ05_000974"
     CDS             8169..8735
                     /gene="satP"
                     /locus_tag="JNZ05_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HAY8564293.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8770..9357)
                     /gene="mog"
                     /locus_tag="JNZ05_000975"
     CDS             complement(8770..9357)
                     /gene="mog"
                     /locus_tag="JNZ05_000975"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HAY8564294.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9472..10425)
                     /gene="tal"
                     /locus_tag="JNZ05_000976"
     CDS             complement(9472..10425)
                     /gene="tal"
                     /locus_tag="JNZ05_000976"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY8564295.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10704..12152
                     /locus_tag="JNZ05_000977"
                     /pseudo
     CDS             10704..12152
                     /locus_tag="JNZ05_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12222..12998
                     /gene="yaaA"
                     /locus_tag="JNZ05_000978"
     CDS             12222..12998
                     /gene="yaaA"
                     /locus_tag="JNZ05_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HAY8564296.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13151..13447)
                     /locus_tag="JNZ05_000979"
     CDS             complement(13151..13447)
                     /locus_tag="JNZ05_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY8564297.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13661..14947)
                     /gene="thrC"
                     /locus_tag="JNZ05_000980"
     CDS             complement(13661..14947)
                     /gene="thrC"
                     /locus_tag="JNZ05_000980"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAY8564298.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14948..15880)
                     /gene="thrB"
                     /locus_tag="JNZ05_000981"
     CDS             complement(14948..15880)
                     /gene="thrB"
                     /locus_tag="JNZ05_000981"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HAY8564299.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15882..18344)
                     /gene="thrA"
                     /locus_tag="JNZ05_000982"
     CDS             complement(15882..18344)
                     /gene="thrA"
                     /locus_tag="JNZ05_000982"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HAY8564300.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            complement(18425..18490)
                     /gene="thrL"
                     /locus_tag="JNZ05_000983"
     CDS             complement(18425..18490)
                     /gene="thrL"
                     /locus_tag="JNZ05_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HAY8564301.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18704..19390)
                     /locus_tag="JNZ05_000984"
     CDS             complement(18704..19390)
                     /locus_tag="JNZ05_000984"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY8564302.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSSDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19790..19930)
                     /locus_tag="JNZ05_000985"
     CDS             complement(19790..19930)
                     /locus_tag="JNZ05_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564303.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20026..20742
                     /gene="arcA"
                     /locus_tag="JNZ05_000986"
     CDS             20026..20742
                     /gene="arcA"
                     /locus_tag="JNZ05_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HAY8564304.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20801..22153)
                     /gene="creD"
                     /locus_tag="JNZ05_000987"
     CDS             complement(20801..22153)
                     /gene="creD"
                     /locus_tag="JNZ05_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HAY8564305.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22211..23635)
                     /gene="creC"
                     /locus_tag="JNZ05_000988"
     CDS             complement(22211..23635)
                     /gene="creC"
                     /locus_tag="JNZ05_000988"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HAY8564306.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNTIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRNFT"
     gene            complement(23635..24324)
                     /gene="creB"
                     /locus_tag="JNZ05_000989"
     CDS             complement(23635..24324)
                     /gene="creB"
                     /locus_tag="JNZ05_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HAY8564307.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGVDDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24337..24810)
                     /gene="creA"
                     /locus_tag="JNZ05_000990"
     CDS             complement(24337..24810)
                     /gene="creA"
                     /locus_tag="JNZ05_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HAY8564308.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25021..25890
                     /gene="robA"
                     /locus_tag="JNZ05_000991"
     CDS             25021..25890
                     /gene="robA"
                     /locus_tag="JNZ05_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HAY8564309.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGKFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25887..26534)
                     /gene="gpmB"
                     /locus_tag="JNZ05_000992"
     CDS             complement(25887..26534)
                     /gene="gpmB"
                     /locus_tag="JNZ05_000992"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HAY8564310.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26586..27107
                     /gene="yjjX"
                     /locus_tag="JNZ05_000993"
     CDS             26586..27107
                     /gene="yjjX"
                     /locus_tag="JNZ05_000993"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP phosphatase"
                     /protein_id="HAY8564311.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(27192..27518)
                     /gene="trpR"
                     /locus_tag="JNZ05_000994"
     CDS             complement(27192..27518)
                     /gene="trpR"
                     /locus_tag="JNZ05_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HAY8564312.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27608..29545)
                     /gene="sltY"
                     /locus_tag="JNZ05_000995"
     CDS             complement(27608..29545)
                     /gene="sltY"
                     /locus_tag="JNZ05_000995"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HAY8564313.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTHQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29756..31423
                     /gene="ettA"
                     /locus_tag="JNZ05_000996"
     CDS             29756..31423
                     /gene="ettA"
                     /locus_tag="JNZ05_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HAY8564314.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLDQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGSQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31731..32963)
                     /gene="nadR"
                     /locus_tag="JNZ05_000997"
     CDS             complement(31731..32963)
                     /gene="nadR"
                     /locus_tag="JNZ05_000997"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HAY8564315.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32984..34366)
                     /gene="radA"
                     /locus_tag="JNZ05_000998"
     CDS             complement(32984..34366)
                     /gene="radA"
                     /locus_tag="JNZ05_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAY8564316.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34415..35383)
                     /gene="serB"
                     /locus_tag="JNZ05_000999"
     CDS             complement(34415..35383)
                     /gene="serB"
                     /locus_tag="JNZ05_000999"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HAY8564317.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAARSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVLANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35489..36133
                     /locus_tag="JNZ05_001000"
     CDS             35489..36133
                     /locus_tag="JNZ05_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HAY8564318.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTQLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36161..37177
                     /gene="lplA"
                     /locus_tag="JNZ05_001001"
                     /pseudo
     CDS             36161..37177
                     /gene="lplA"
                     /locus_tag="JNZ05_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37209..37472
                     /locus_tag="JNZ05_001002"
     CDS             37209..37472
                     /locus_tag="JNZ05_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8564319.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37633..38352)
                     /gene="deoD"
                     /locus_tag="JNZ05_001003"
     CDS             complement(37633..38352)
                     /gene="deoD"
                     /locus_tag="JNZ05_001003"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAY8564320.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38409..39632)
                     /gene="deoB"
                     /locus_tag="JNZ05_001004"
     CDS             complement(38409..39632)
                     /gene="deoB"
                     /locus_tag="JNZ05_001004"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY8564321.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLIRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39684..41006)
                     /gene="deoA"
                     /locus_tag="JNZ05_001005"
     CDS             complement(39684..41006)
                     /gene="deoA"
                     /locus_tag="JNZ05_001005"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HAY8564322.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MVLCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41133..41912)
                     /gene="deoC"
                     /locus_tag="JNZ05_001006"
     CDS             complement(41133..41912)
                     /gene="deoC"
                     /locus_tag="JNZ05_001006"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAY8564323.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRYMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42170..43711
                     /locus_tag="JNZ05_001007"
     CDS             42170..43711
                     /locus_tag="JNZ05_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HAY8564324.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43683..44546
                     /gene="yjjW"
                     /locus_tag="JNZ05_001008"
     CDS             43683..44546
                     /gene="yjjW"
                     /locus_tag="JNZ05_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HAY8564325.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEVTTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44760..45542)
                     /locus_tag="JNZ05_001009"
     CDS             complement(44760..45542)
                     /locus_tag="JNZ05_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY8564326.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45539..46612)
                     /locus_tag="JNZ05_001010"
     CDS             complement(45539..46612)
                     /locus_tag="JNZ05_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HAY8564327.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGGSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46734..46895)
                     /locus_tag="JNZ05_001011"
     CDS             complement(46734..46895)
                     /locus_tag="JNZ05_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HAY8564328.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(47022..47627)
                     /gene="osmY"
                     /locus_tag="JNZ05_001012"
     CDS             complement(47022..47627)
                     /gene="osmY"
                     /locus_tag="JNZ05_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HAY8564329.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKDYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(48020..49609)
                     /gene="prfC"
                     /locus_tag="JNZ05_001013"
     CDS             complement(48020..49609)
                     /gene="prfC"
                     /locus_tag="JNZ05_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAY8564330.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49700..50377)
                     /gene="yjjG"
                     /locus_tag="JNZ05_001014"
     CDS             complement(49700..50377)
                     /gene="yjjG"
                     /locus_tag="JNZ05_001014"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HAY8564331.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50392..50838)
                     /gene="rimI"
                     /locus_tag="JNZ05_001015"
     CDS             complement(50392..50838)
                     /gene="rimI"
                     /locus_tag="JNZ05_001015"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAY8564332.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50807..51220)
                     /gene="holD"
                     /locus_tag="JNZ05_001016"
     CDS             complement(50807..51220)
                     /gene="holD"
                     /locus_tag="JNZ05_001016"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HAY8564333.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51323..52354
                     /gene="rsmC"
                     /locus_tag="JNZ05_001017"
     CDS             51323..52354
                     /gene="rsmC"
                     /locus_tag="JNZ05_001017"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HAY8564334.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRT
                     IMTRQAKKG"
BASE COUNT        12527 a        14204 c        13174 g        12644 t
ORIGIN      
        1 tatcagtttc atcatataag atttgactgc atccagggcg agcaaggtga attagttttt
       61 gaacccagtg acgttgaata ttactttgaa ccagtgacga tacaggagtc aggaaccagc
      121 atattaaaaa atgagttaga aaacagtgag gacggtgcgg gtcaaatagg ctttcaactc
      181 tccaatgatg gtacgaatga aatccagtat ggtaaaagca attactactc ttttcagcat
      241 ccccatgaag gaagtaatca gattcctcta tttattcgcc cgcgtacata tggaaacaat
      301 gtctcaagcg ggcaaataat gagccgagtc aaaatcgtgg taatgtacaa ctaatctatc
      361 tcccccacct taactttggg ggagctgccg attaacgcac cgctggacta aaaagcgcag
      421 aataatccgt attgcagatt cgctgtaccg ccgggtgctg aatcatccgc tcagcaaaaa
      481 tagcatggta ctcttccatc acattctcga cgcgaccaat ttctacgaca gttttatcgg
      541 cataaaaatc atatgcataa agcgttgggg caacgaagat tgcattgtgc atcgcaccaa
      601 aagctttcat caaagcggca tcatcaaact cgccgaggat ttctacgttt aatccctggg
      661 agttaaacca gttaagcaat ttgcgcccta acattgaacg tcgcccagga atcaaaagtc
      721 gccgttcttc cagacaagcc gggaacggtt tttctggtgg tggatttgta caccagaaac
      781 tcacgccaca ttcgccaatt ctcacggaga acaggccttc ctgctgcgta gagtctatcg
      841 gacagtcaga aatgatcata tccagtttat gctgacttaa ttgctccagc agcatttcgt
      901 gggtggattc gaagcagcga agatgaatgg gctcgccttc taccactgcg gcgttaagta
      961 cgctactgac caggcgtttg gaaagtgcat cagccacgcc aacgtcaaac aataaattgg
     1021 attctttgcg atagttcaca atatccagca tttcctggct taaggtgaac attttatcgg
     1081 catagcgata gaccagttct cccagctcgc tgggttcgag accacgtccc ttgcgtttaa
     1141 ataatttgcc ttgcaggcgc tcttccagcg ctcgaatctg tccggtaatg gtttgtggag
     1201 ttaaataaag cgcctccgct gcgccaacca cggaaccttc tttatagaca tgccagaagt
     1261 aatacaagtg gttgtaattg atatgagaca tgctcatttc tctccctgat aacaatgaaa
     1321 agggagccgt ttatggctcc ccggtaaacc gtcctgtcaa actgatggac gcaaacgaac
     1381 gcgtaaccag ctgtatccaa ttaccgccga agagatagaa ccgaccagga taccgagttt
     1441 cgcccagtta atcagttctg gatctacgct accaaaggcc aggctggcaa taaagataga
     1501 catagtaaaa ccgataccgc acaggatccc caccaccata atttgctgat aagtcgttcc
     1561 ctcaggcaga tgcgccagtt tcaaacgcag cgccaaccag cagaacagac taatccccag
     1621 cggtttgcca atcagcaagc cagcgatgat ccccaatggc agaatggagg tcaagccatc
     1681 cagcgtgacg ccttgcagtg aaacgccagc attagaaaat gcaaacagcg gcaaaatcag
     1741 atacgccacc cacgggtgca acacatgctc cagtcgcttc gctggagaac gcccatgctt
     1801 ctctttcaaa ggaataaaga agccgacaat tacccccgcc agagttgcgt gaacccccga
     1861 tttcaacacc gcagtccaca acaccacgcc aacaagaata tagacgcccg tgcggcgtac
     1921 accacacaga ttcaataccg cgagtaccgc aattgctaca gccgcgacgc caagagaggc
     1981 catcgataag tcattagtgt agaacaatgc gatgataatg atggccccaa gatcgtcgat
     2041 aatagccaga gccatcaaaa agatcttcag cgctaacgga acacgacttc ccaacagcgc
     2101 cagtacacca agtgcaaaag caatgtcagt agccgccggg atcgcccacc cttcgcgggt
     2161 aatcggatcg gcatagttaa aaggcagata gagtaatgcc ggcacaatca tcccaccaat
     2221 agcggcgata actggaaatg cggcctggcg taagctggct agcgatcctt gcatcagttc
     2281 acgtttaact tccagaccga ctaacaggaa aaataccgcc atcagcgcgt catttatcca
     2341 taacagcatg tttttgttga tttcgagtga accaacccgg agctgaaccg gcgtctccag
     2401 aaagtcgtga taccatccac tggttgcgcc gctgttggcc ataatcatcg ccaggatagc
     2461 ggcaatgata agtataatgc ctcccgaggc atcactgcta aagaatcgat gcagatgttt
     2521 cactttttat ttctctttca ggtgaataga tcgtttttac tattttaccc cgcacgaatc
     2581 atcgttaaaa tcagattata gtgtaggaat aattcgtttt ttacgagcta ttgaagaatt
     2641 tgcgccatca gcggtgagat gcgtttttga cggctgtaag cgctcacgag tgaccctaag
     2701 cccagataat catagcttaa ggtgaggatt cctgagggat taattaggaa aagtaataat
     2761 taatttgttg tcagtcggcg atgcttgtta ggccaatatt tcaaaaataa tccagcttgt
     2821 cgtcaataat tgcgatgaag agataaatta aagttaaata attaaatcat taaaaaatta
     2881 cgccacaaaa atgcgaggcg tcttcaggat tagaatcgat aaaacaaaat gggttaaaga
     2941 gagcgatata atagcggcgg gtgcctgagg ctgtctgtct caggcattag ctgaacggca
     3001 gatagagaaa agccccgagt gatattttac catcaacccg aggcctccta tatgctgaac
     3061 acatgtagag tgcctcttac tgaccgtaag gtcaaggaga agagagcaat gaagcagcat
     3121 aaggcgatga ttgtcgccct gatcgtcatc tgtatcaccg ccgtagtggc ggcgctggtg
     3181 acgagaaaag acctctgtga ggttcacatc cgaactggcc agacggaggt tgctgttttc
     3241 acggcttacg aatccgagta agagcaacgg cggggagtga tccccgccac tttacaggtg
     3301 ctcgcatatc ttcaacgcac cctattttta cccaggcctg cccacgggca ggcttttggg
     3361 gaggttagcg ggtcaggtcg tcaaaaaact tcttcacacc atcaaagaag ctctttgagc
     3421 gcgggctgtt gtgctcgccg gttgggccac cgaagctttc ttgcagctct tgcagcagct
     3481 gtttctgctt ttcgttcagg cctaccggtg tttcgacgac aacgcggcac agcaaatcac
     3541 cctgtgcacc accgcggaca gacttgacgc ctttaccgcg catacggaat agcttaccgg
     3601 tctgggtttc gccaggcact ttcagtttga cgcgaccatc aagggtcggt acttcgattt
     3661 cgccacccag cgccgccata gcgaagttga tcgggacttc gcaatacagg ttgttgcctt
     3721 cacgctcgaa aatcgggtgc tgtttaacct gaacctgaac gtacagatcg cctgccggtg
     3781 cgccatgctc gcccgcttca ccttcgcccg caagacggat gcggtctcca gtgtccaccc
     3841 ctgccgggat tttaacggac agcgttttgc tgcgctcaac acgaccatga ccatgacatt
     3901 tgttgcacgg atctttgatc agcgtaccgc ggccctgaca gtgtggacag gtctgctgta
     3961 cagcgaagaa tccctggcgc atctgcacct gaccagaacc atgacaggtc ggacaggtct
     4021 gcggctgtgt acctggtttt gcaccgctac cgtggcaaac gtcacactct tccagagtcg
     4081 gaatgcggat ttctttggtc acgccacgta cagcttcttc gagggtgagc tccatgttat
     4141 agcgtaaatc agcaccgcgc gccgcacgtt gacgaccacg tccgccgcca aaaatatcgc
     4201 cgaaaacgtc accaaaaata tcgctgaagt ctgcgccgcc gccaaaacca ccgccgccca
     4261 tgcctccttg ctcaaacgca gcatgaccat actgatcgta tgccgcacgt ttttgcgagt
     4321 cggtcagaac ttcataagct tccttgatct ctttaaattt cgcctcggcc tctttgtcac
     4381 cctggttacg gtccgggtgg tatttcatgg ccaggcgttt gtaggccttt ctgatttcac
     4441 gctcttccgc tgttttggaa acgcctaaaa tctcgtaata atcttgctta gccatctttt
     4501 ttaaattgcc cctagatgaa tgcacgggcg gagaggaaat tccccttcgc ccgtgtcagt
     4561 ataattaccc gtttataggg cgattatttt ttgtctttga cttcttcaaa ttcagcgtcg
     4621 acaacatcgt catctttcgc gttgtttgca gaagcatcag caccggcagt ctgctgctgg
     4681 gcatgttgct gctgggcgat ttccatcagt ttctgggaaa cctgtgccag ttcctgcatt
     4741 ttcgcttcga tagcggcttt gtcttcacct ttcagagcag tttccagtgc agtcagcgca
     4801 gactcgatag cagttttgtc gtcagccggc agtttgtcgc ctgcttcttc aacctgctta
     4861 cgggagctgt gcagcagatg gtcgccctgg ttgcgagtct gtaccagctc ttcaaactta
     4921 cggtcagctt cggcgttagc ttctgcgtcg cgtaccattt tctggatttc atcttcgttc
     4981 agaccagaag aagccttgat ggtgatcttc tgctctttac cgctgttttt atctttcgcg
     5041 gaaacgtgca ggataccgtc agcatcgata tcgaaggtaa cttcgatctg cggcatgccg
     5101 cgcggtgccg ggttgatacc atctaggttg aactgaccca gagatttgtt atcagtcgca
     5161 cgtttacgtt caccctgcag cacatggatg gttaccgcag actggttgtc ttcagcggta
     5221 gagaacacct ggctgtgctt ggtcgggata gtggtgtttt tcgcgatcag cgtcgtcatc
     5281 acaccgccca tggtttcgat acccagagac agcggggtaa cgtccagcag cagtacgtct
     5341 tttacgtcac cagtcagaac accaccctga acagcagcac cgattgctac agcttcgtcc
     5401 gggttaacgt ctttacgcgg ctctttacca aagaactcag caactttctt ctgaaccatt
     5461 ggcatacgag tctgaccacc aacgaggata acgtcgtcga tatcagatac ggacaggcca
     5521 gcatcctgca gtgcaacttt cagcggctca atggaacggt ttaccagatc ttcaaccagg
     5581 ctttccagtt tcgcacgagt cactttgatg ttcatgtgtt tcggaccggt cgcgtctgca
     5641 gtgatgtatg gcaggttaac gtcggtctgc tgagcggaag acagttcgat tttcgctttt
     5701 tctgccgctt ctttcaggcg ctgcattgcc agcggatcgt tgcgcaggtc aatgccctga
     5761 tctttcttga attcttcaac cagatagttg atcagacggc tgtcgaagtc ttcaccaccc
     5821 aggtgggtat caccgttggt tgccagaact tcgaaggttt tttcgccgtc aacttcgtcg
     5881 atttcgataa tagaaatatc gaaagtacca ccccccaggt cataaaccgc gatagtacgg
     5941 ttgccagtgc ctttgtccag accgtaagcc agcgcagctg cggtcggttc gttgatgata
     6001 cgttttactt ccagaccagc gatacggcct gcgtctttgg ttgcctgacg ctgagcatcg
     6061 ttaaagtatg ccggtacggt gataacagct tcagttaccg gttcacccag gtaatcttca
     6121 gcggttttct tcattttttt cagcacttca gcagaaatct gcggcggtgc cattttctgg
     6181 cctttaactt cgacccatgc gtcgccgtta tcagcagcaa taattttgaa cggcatgatg
     6241 gaaacatcac gctgtacttc ttcgtcctgg aagcggcggc caatcaggcg tttaatcgca
     6301 aacagggtgt tttgcgggtt cgtcactgcc tgacgtttag ccggctgacc aaccagagtt
     6361 tcaccatcct gggtataggc aatgatagaa ggcgtggtgc gatcgccttc ggcgttctcc
     6421 agcacgcgag gaatggtgcc atccataatc gctacacaag agttggtagt acccaggtcg
     6481 ataccaatta ttttacccat ctaaacgtct ccactatata ttcggtcatc atgtggttgt
     6541 gagtctgtaa taggggcgaa acgtctggtt tcaactgccc aatttcattt ttttgcagac
     6601 tcactcactg cggttgacta ctaaatgggg tcgtaaccca cgtcatcaag ggggagatgc
     6661 aaaaattttt tgtgcttttg cgcagaaatt tcgcgatatg gcaggacaag acgtaaaatt
     6721 gacagcaatt ttattcatat aaagaatgaa tcgcagccaa ggcattcatc aaaaaattgt
     6781 aataaaaaga aaagattacg tgcctgaatc ttctctttat cagcagtaaa ctagtgggta
     6841 ttcatccccc tacctcttcc cactaagaga atccttatga aatccgtttt tacgatttcc
     6901 gccagcctgg cgattagcct gatgctgtgc tgcacggcgc aggcaaacga ccataaactc
     6961 ctcggcgtca ttgcaatgcc gcgtaacgaa accaacgatc tggcgctgaa acttcctgtt
     7021 tgtcgcattg tgaaacgcat acaactctct gccgaccatg gcgatttaca gttaagcggt
     7081 gcatcggttt atttcaaagc cgcccgtagc gccagtcaga gcctgaatat tccttcagaa
     7141 ataaaagaag ggcaaaccac tgactggatc aacattaaca gcgataacga caataaacgc
     7201 tgcgtctcaa aaatcacctt ttccggtcat acggtgaact catcggatat ggccacgctg
     7261 aaaattatcg gcgacgacta acgccagaat atagaagcca caaaaaatga atgttaatta
     7321 cctgaatgat tcagatctgg attttcttca gcattgtagt gaggaacagt tggcaaattt
     7381 cgcccgattg ctcacccata atgaaaaagg caaaactcgc ctctccagcg tactgatgcg
     7441 caacgaactg tttaaatcga tggaagggca tcccgagcaa catcgccgca actggcagct
     7501 gattgccgga gaattacagc attttggtgg cgatagtatc gccaacaaac tgcgcggaca
     7561 cggtaaattg tatcgggcca ttttgctcga tgtttcaaag cgattgaagc tgaaagccga
     7621 caaagagatg tctacgtttg aaattgagca acagttactg gaacaatttc tgcgtaatac
     7681 ctggaagaaa atggacgagg aacataagca ggagtttctg cacgcggtcg atgccagggt
     7741 gaatgagctg gaagagctgc tgccgctgct gatgaaagac aaattattgg caaaaggtgt
     7801 gtcgcatttg ctttccagcc aactgacccg cattttacgc acccacgcag caatgagcgt
     7861 acttgggcat ggtttgctgc gcggagcggg gctgggaggc cctgtgggcg cagcattaaa
     7921 tggggttaaa gcggtcagcg gcagcgccta tcgcgtgacg attccagccg tactgcaaat
     7981 cgcctgcctg cgccggatgg ttagcgccac tcaggtctga taccggaaac cggggaaaat
     8041 tttcattttc ccctcaaaag atcgtagaca ctgccccact ggctgattat tatgccgcgc
     8101 cctgaaaaca ctacagttat tcagggaaat tatttcacca ttcattcgat gatgattttt
     8161 gaggaattat gggcaacact aagttggcta atccggcacc gctgggcctg atgggcttcg
     8221 gcatgaccac cattctgctt aacctgcaca acgtgggtta tttcgctctg gacggtatta
     8281 ttcttgccat gggcattttc tacggcggca tcgcgcaaat ttttgctggt ctgctggagt
     8341 acaaaaaagg caacactttc ggtttaaccg cattcacctc ttacggttct ttctggctga
     8401 cgctggttgc gattctgctg atgccgaaac tgggtctgac cgatgcgcca aatgcacagt
     8461 tccttggtgt ctacctgggt ctgtggggcg tatttacgct gtttatgttc ttcggcactc
     8521 tgaaaggcgc acgcgtcctg caattcgttt tctttagcct gaccgtgctg tttgccctgc
     8581 tggcgatcgg taacattgcc ggtaatgccg cgatcatcca ctttgccggc tggattggtc
     8641 tgatctgcgg tgccagcgca atctatctgg cgatgggtga agtgctgaac gagcagtttg
     8701 gtcgcaccgt tctgccgatt ggtgaatccc actaattgtc ttgagatccc cccgctcggg
     8761 gggatttttt tattcgctaa cgtcgcgtct tgcactcttc ggtctgaatg cagcaaccac
     8821 ttctggtgcc gtttcaacgt atggcccttc cagcaactga atgcagtacg gtacgctggc
     8881 aaaaatacca tgcaccacaa cgttaccctc agcgtccttc acaccttcca gcgtctcttt
     8941 aatagacttc ggctgaccgg gtaagttaag gatcagcgcc tgtttacgaa tcacgcccac
     9001 ctgacgcgaa aggatcgcag ttggtacaaa atgcaggctg atctggcgca tctgttcacc
     9061 aaagccaggc atctcgcggt ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg
     9121 cgccgggcca gttccgcccg tggtgagcac cagatggcaa ctcatttcat ccaccagctc
     9181 acacaacgtt tgctcgatga tcgcctgctc atcggggatt aagcgggttt ccagttcaaa
     9241 cggcgtggtt agcgccgatg tcagccattc ttccagcgct ggaatgcctt tatcctgata
     9301 aacgccgctg gatgcgcgat cagagatgga aactaagcca atacgtaaag tattcatcaa
     9361 gatttttccg tttaaacagt ctcgttaaac agaatgatac actgcgaagg gagtgacaga
     9421 caggcttcag aagaggtagc gtgaccgact tcccggtcac gctaagaatg attacagcag
     9481 atcgccgatc attttttcca gtttttcctg gtcaatagca aacttacgga taccttccgc
     9541 cagtttatct actgccattg gatcctggtt gtgctgccac aggaactcgg actcagtgat
     9601 acgcgccgga cgcgctttca cttcgccggt gtaagacagt ttacgttcga tagccccttc
     9661 gctctccgcc agctctttca gcagtgccgg tgcgatggtc agacggtcac agcctgccag
     9721 ttccagaatt tcgccgatgt tacggaagct tgcgcccata accacggttt cataaccgtg
     9781 ctctttgtag tactggtaga tttcagatac agaaaccacg cccggatctt ctgccggagc
     9841 gtactctttc ttatcggtat tcgctttgta ccagtcaaga atacggccaa caaacggcga
     9901 gatcaggaac acgcccgcct ccgcacaagc acgagcctga gcgaaggaga acagcagggt
     9961 caggttacag ttgatacctt ctttttccag ctgttctgca gcacggatac cctgccaggt
    10021 agaagccagt ttgatcagaa tacgatcgtt gctaatacct gcatcgttgt agagtttgat
    10081 caggcgtttt gctttcgcaa ttgacgcttc ggtgtcatag gaaagacgcg catcaacttc
    10141 agttgagata cggcccggaa ccagtttcag gatttccaga ccaatattta ctgccagttt
    10201 gtcggtcgcg tccacgatct gctgcgcgcg atcgttgctc tgctgtttcg cccaggcgac
    10261 agcatcatca atcaacttac ggtattccgg aatctgcgct gcgttaagaa tgagagaagg
    10321 gttggttgtg gcatcctgcg gttgatacag cttcattgcc gcgatgtccc cagtgtcggc
    10381 cactacggtg gtgtactgac gaagggaggt caatttgtcc gtcatgatag tatttctctt
    10441 taaacagctt gttaggggga tgtaaccggt ctgccctgat gatatcacga cgcaacgtca
    10501 gcgcaaccgc cgacaagacg gttatcgcca ggcgagactg tttcggattt ctgacactgc
    10561 gttgatatcg cccgccattt ttatacaaaa cctcatgtat gctacgcaga agttatcaag
    10621 tacctcgtag cgtatatact tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc
    10681 gaccggcaat aagagggata tgcatgccag attttttctc ctttattaac agcgtccttt
    10741 ggggatcggt aatgatttac ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg
    10801 gatttgtgca gtttcgctac atccgccagt ttggcaaaag tcttaaaaat agcattcatc
    10861 cacagccagg cggtttaacc tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg
    10921 gtagcggcaa cctggccgcc gttgcgctgg caattaccgc cggtggacca ggtgccgtct
    10981 tttggatgtg ggttgctgcg tttatcggca tggcgacctc gtttgccgaa tgttcccttg
    11041 cacaacttta taaagaacgt gacgtcaatg ggcagtttcg tggcggaccg gcatggtata
    11101 tggcgcgcgg gctggggatg cgctggatgg gcgttctgtt cgccgtcttt ttgctcatcg
    11161 cctacggcat aattttcagc ggagttcagg cgaacgccgt tgcccgagcc ctgagttttt
    11221 cttttgattt tcctccgctg gtgacgggca ttattctcgc tgtctttgct ctgctggcaa
    11281 tcactcgcgg tcttcatggc gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga
    11341 taatctgggt actgaccagc ctggtgattt gcgtaataaa tatcgggcaa cttccccacg
    11401 tcatttggtc tatttttgag agtgcttttg gctggcagga agcggcaggc ggcgcggcgg
    11461 gatatacctt aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg
    11521 cgggaatggg atcgacgcca aacgcggcag cggcagcggc agcggcagcg gcagctgcat
    11581 cctggcctcc gcatccggca gcacaaggga ttgtccagat gattggcatt tttatcgaca
    11641 ccctggtcat ctgtacggca agcgccatgc tgatattact ggcgggtaac ggcacaacct
    11701 acatgccgct ggaaggtatt cagcttatcc agaaggcgat gcgggtgcta atgggttcct
    11761 agggtgctga atttgttacc ctcgtggtta ttctgtttgc cttcagctcc atcgttgcca
    11821 actacattta tgccgaaaac aatctcttct ttttacgcct gaacaaccct aaagcgatct
    11881 ggtgtttgca gatctgcacc ttcgcaacgg tcatcggcgg caccttgcta agccttccgc
    11941 tgatgtggca actggcagat atcataatgg cctgcatggc tattaccaat ttgaccgcca
    12001 ttttactgct ctcgcctgtg gttcatacca ttgccagtga ttatctacgc cagcgtaaac
    12061 tcggcgtgcg cccggtgttt gatccgttgc gttatccgga tatcggtcgc cagctttctc
    12121 cggacgcgtg ggatgatgtt tcgcaggagt aatcacaact atcgatcaac tcattctctt
    12181 tttttgctaa agtcggcata aatttcctgc aaggactgga tatgctgatt cttatttcac
    12241 ctgcgaaaac gcttgattac caaagcccgt tgaccaccac gcgctatacg ctgccggagc
    12301 tgttagacaa ttcccagcag ttgatccatg aagcgcggaa actgacgcct ccgcagatta
    12361 gcacgctgat gcgcatcagc gacaaactgg cgggtatcaa cgccgctcgc tttcatgact
    12421 ggcagccaga tttcacgccg gcgaatgccc gccaggcgat tctggcgttt aaaggtgatg
    12481 tctacaccgg cttgcaggcc gaaaccttca gcgaagacga tttcgatttt gcccaacagc
    12541 atttgcgaat gctttccggc ttgtatggcg tactgcgccc gctcgattta atgcagcctt
    12601 atcgtctgga aatggggatc cgtcttgaga atgcccgagg gaaagatctg tatcaattct
    12661 ggggagatat catcaccaac aagctgaacg aggcgctcgc agcacaaggc gataatgtgg
    12721 tgattaatct ggcgtcagat gagtatttta aatcggtgaa gccgaagaaa ttgaatgccg
    12781 agattatcaa gccggtgttc ctcgatgaga agaacggcaa atttaagatc atcagcttct
    12841 acgctaagaa agcacgcggt ctgatgagtc gtttcattat tgaaaatcgg ctgaccaaac
    12901 cagagcaact gactggtttt aatagcgaag gttacttctt tgatgaagat tcctccagca
    12961 atggcgaact ggtgtttaaa cgctacgagc agcgttaagt ttgtagtcaa tgccggatgc
    13021 ggcgtgaacg ccttatccgg cctacaaaag catgcagatt caatatattg cagaattaac
    13081 gtaggcctga taagacgcgc cagcgtcgca tcaggcgctg aatgccggat tgttacccgg
    13141 catttgtcat ttagcggtga tgtttgcctg gaccatgacc gccgtgatga tcatgaggag
    13201 ctttcttatg gtggcgcggc ggtggcggcg gtccgtgtgg gtgccagcga ttgcctcgcc
    13261 attcataatg ttgtttccac cagccgtggt cgcgccagtg acctccatcc cagtaatagc
    13321 cacgattatc acgatcgcct atctgtaatt ttactgacgg gactaacgta atttccgcag
    13381 cctgtgctgc catgggagcg accagaacca gggaaagtgc gagtacgata gattgcatct
    13441 ttttcacagg ttactccttt tatcattgcc cgttatgggt cgatatccgg agattacgcg
    13501 atgagaacga cggttgttat tctccgcaat cctaatctct aagttaccgc atttattgat
    13561 aatttctcct ttttccctgt cgtttttctc cataatttct tcataaaaaa gccgggctgc
    13621 ataaaagcaa acccggcctg attgagataa tgaatagatt ttactgatga ttcatcatca
    13681 atttacgcaa cgcagaaaaa tcggcgggca gattatgtga aagcaagggt aaatcagcac
    13741 gttctgccag ctcttttggc agatccaacg tttcaccgag aatcgcttcc acgctctctt
    13801 taaatttcgc cggatgcgcg gtgccgagga acaagccata ttcgcctgga ttcaactggt
    13861 cacgcagcgc acgatacgct acggcagcgt gcggctccga ggtgtagccc agttctttta
    13921 actcacgcat tgtctgttgc gtggtttcat catccacggc tgcataaccc agctctttca
    13981 gttgccagat tttgcggcgg aacaactctt ccacacgcgg ccagttgttc ggctgactca
    14041 cgtccatcgc gttggataac gtcgcctgag tcgctttggg tgaccactga ccgtcgtgca
    14101 ggaaacgtgg cacggtatcg ttcacgttgg tcgcagcaat aaaacgtttc accggcagac
    14161 cgaatgactt cgccagcaga cccgccgtca aatcgccgaa gtttccgctt ggcaccgaga
    14221 caaccagctg gttgcgcgcc tcctgcggca gctgcgcaac agcttcaaag tagtagcaaa
    14281 tctgcgccag caaacggctg atgttaatcg agttagccga gtttaacccc agcgccactt
    14341 tcagttcttc atcatcaaac gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt
    14401 cgatggcaac agtttcgata ttgccgccca acgtacagaa cagtttttct tgcagtggac
    14461 tgattttgcc tcgtggatag aggataacca ctttcacatt cggcaaaccg tagaaagcat
    14521 gagccactgc agctccggta tcaccggagg tcgcggtcag aatggtcact ggcttatcat
    14581 ccgcaatatg ggtcagcatt tgtgccataa agcgaccgcc gaaatcttta aatgccagcg
    14641 ttggcccgtg gaacaattcc agacaaccga catcgctttc aacattggcg accggagccg
    14701 ggaaggcaaa cgccgcgcgc acgcgctctt ccaggatttc ctgtgggatt tcatcaccaa
    14761 taaacgccga gaggatcttc gcactgcggg tgacaaaatc cagcttcagc atctcatcaa
    14821 tttcagtcag gctgaattcc ggcaggtcgt gcggaaaaaa cagcccctga tttttgccca
    14881 acccctgggt tacggcttgc gcaaagctga cctgctcgtt gtgatctttc agattgtaga
    14941 gtttcattta gttttccagt actcgtgcgc ccgccgtatc cagccggcaa atatgaacaa
    15001 aaccttcctg attttgcagg tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat
    15061 ccggcttgtc acatagagcg aacaaggtcg ggccggagcc ggagataccg ctcgctaccg
    15121 cgccgatttc cgcgaccgcc tgccgcgcct gccggaagcc aggcagtaac cgttcacggt
    15181 agggttcagc gataacatct ttcatcagct tcgcggcaag ctcaggctga cgggaatagc
    15241 aggcgtgaat gaagccagcc agatgtcgcc cgtgcgcaat gcaatcctgg cggcgatact
    15301 gcgccggtaa aatagcccgg gcttctgccg tcgagacttt aattcccgga tacgccagca
    15361 cccacagcca ctcatcaaac cctggcactt gctggctgat gatgtcgttt tcttcgatca
    15421 tcaactgcat accaccaaga aaacacggtg ccacgttgtc gtaatgaatg ctgccggaga
    15481 tacgtccttc cagctcgccc atcaaagcca gcaaacgagt gtcattaagc ggcttgccgc
    15541 agtgttcatt catcgccatc agcgccgcga ccaccgaaca ggcgctggag cctaagcccg
    15601 aaccgatcgg catattcttt tccagagtca tcgccactgg aatttgcttg ccaagctcct
    15661 ggcaaaaacg ctcccagcac tgataaacga tattttcccg tggttctgac ggcagcttat
    15721 cggcaaagcg tccgaggttg ttgagactga atgtctctgc cgcctcaacc gtgactacat
    15781 ctccgagcaa tgcaccatca acgggtgcca ccgccgcccc gagcacatca aacccgacgc
    15841 tcatattggc actggaagcc ggggcataaa ctttaaccat gtcagactcc taacttccat
    15901 gagagggtac gtagcagatc ggcaaagaca ccggcagcgg taacgtcatt gcccgcacca
    15961 tatccgcgca gcaccaacgg cagcggctga taatagtggc tataaaaggc cagggcgttt
    16021 tcgccatttt tcactttgaa cagcggatca ttcccatcca cttcggcaat cttcacgcgg
    16081 cagacaccat cttcatcaat attgccaaca tagcgcaaaa cttttccttc atcacgggcc
    16141 ttcgccacgc gcgcggcaaa gagatcgtcg agctgtgaca gattcgccat aaaagcggca
    16201 acatcaccct cggcgttaaa ctctgcgggc agcacaggtt caatttcaat atccgccagc
    16261 tccagttcac gtcccgtttc acgagcgaga atcaatagtt tacgcgccac atccatacca
    16321 gaaagatcat ctcgcgggtc cggttcggta taacccattt cccgcgccag cgtggtcgcc
    16381 tcggagaaac tcatgccttc gtctaacttg ccgaagatat aagaaagcga acctgaaaga
    16441 atgccggaga acttcaccaa ttcatcacct gcattgagca gattttgcag gttctcaata
    16501 accggtaatc cagccccaac gttggtgtca tagaggaatt tacgccgcga tttttccgcc
    16561 gcataacgca actgatgata gtaatccatc gacgaggtgt tggccttttt gttcggcgtg
    16621 acaacgtgga aaccttcgcg caggaagtcg gcatattgat ccgccactgc ctggctggag
    16681 gtgcagtcaa caatgaccgg gttcagcaga tgatattctt tcacgaggcg aattaagcgc
    16741 ccgagattaa acggctcttt ggcttgtgca agttcttcct gccagttttc cagattaagg
    16801 ccatgcacac tagtgagcag agccttcgag ttggcaacgc cgcagacacg taagtcgata
    16861 tgtttattct tcagccagct ttgctgacgc ttcagttgct ccagcagcgc accgccaacg
    16921 ccaccgacgc caatcacaaa cacttcgata acctgatcgg tattgaacag catctgatga
    16981 gtaacgcgca cgccagtggt cgcatcatcg ttatttacca cgacagagat tgagcgttca
    17041 gaagatccct gagcaatggc gacaatgttg atattggcgc gggccagcgc ggcaaagaat
    17101 ttcgccgaga tcccacgcaa ggtgcgcata ccatcaccta caaccgagat aatggccagc
    17161 cgttccgtca ccgccagcgg ctccagtaag ccttctttca gttccaggta gaactcttcc
    17221 tgcattgccc gttcagctcg cacacagtcg ctttgtggaa cgcagaaact gatactgtat
    17281 tcggaagatg attgcgtaat cagcaccacg gaaatacggg cgcgtgacat tgcagcaaag
    17341 acgcgcgccg ccatgccaac catccctttc atccccgggc cggaaacgct gaacattgcc
    17401 atgttattca gattggaaat gcccttgacc ggtaattcgt cttcatcacg gctggcacca
    17461 atgagcgtac ctggtgcttg aggatttccg gtatttttaa tcaggcaagg gatctggaac
    17521 tgggcgatgg gggtaatggt gcgggggtga agaactttag cgccgaagta ggaaagctcc
    17581 atcgcttcct ggtaggacat cgacttcaac aacctcgcat cgggcacctg acgcgggtcg
    17641 caggtataga ccccgtcaac gtccgtccaa atctcgcaac aatcggcgcg taaacaggca
    17701 gccagcaccg ccgcggagta gtcggaaccg ttacgtccaa gcaccaccag ttcgcctttt
    17761 tcattaccgg cggtgaaacc tgccatcaac accatgtgat cagccggaat gcggcttgcc
    17821 gcaatacggc gggtggactc agcaatatcg acggtagatt cgaggtaatg ccccactgcc
    17881 agcagttttt cgaccggatc gataacggta acgttgtgac cacgcgcttc taacacgccg
    17941 gccataatgg cgatcgacat tttctcgcca cggcaaatca gcgcagcgtt gatgctatcc
    18001 gggcactgcc ccaacaaact aatgccatgc aggacatgtt ttatttgggc aaattcctga
    18061 tcgacgaaag ttttcaattg cgccagcggg aaccccggct gggcggcggc gagtcccgtc
    18121 aaaagttcgg caaaaatacg ttcggcatcg ctgatattgg gtaaagcatc ctggccgcta
    18181 atggtttttt caatcatcgc caccaggtgg ttggtgattt tggcgggggc agagaggacg
    18241 gtggccacct gcccctgcct ggcattgctt tccagaatat cggcaacacg cagaaaacgt
    18301 tctgcatttg ccactgatgt accgccgaac ttcaacactc gcatggttgt tacctcgtta
    18361 cctttggtcg aaaaaaaagc ccgcactgtc aggtgcgggc ttttttctgt gtttcctgta
    18421 cgcgtcagcc cgcaccgtta cctgtggtaa tggtgatggt ggtggtaatg gtggtgctaa
    18481 tgcgtttcat ggatgttgtg tactctgtaa tttttatctg tctgtgcgct atgcctatat
    18541 tggttaaagt atttagtgac ctaagtcaat aaaattttaa tttactcacg gcaggtaacc
    18601 agttcagaag ctgctatcag acactctttt tttaatccac acagagacat attgcccgtt
    18661 gcagtcagaa tgaaaagctg aaaaatactt actaaggcgt tttttatttg gtgatatttt
    18721 tttcaatatc atgcagcaaa cggtgcaaca ttgccgtgtc tcgttgctct aaaagcccca
    18781 ggcgttgctg taaccagtcg accagtttta tgtcatctgc cactgccaga gtcgtcagca
    18841 atgccatgac tcgttcgcgt aaagcttgca gttgatgttg gtctgccgtt gtgtcacttt
    18901 tcgccggttg ttgtattaat gttgctaatt gatagcaata gaccatcacc gcctgcccca
    18961 gattgagcga aggataatcc gccaccatcg gcacaccagt aagcacgtca gccaacgcta
    19021 actcttcgtt agtcagcccg gaatcttcgc gaccaaacac cagcgcggca tggctcatcc
    19081 atgaagattt ttcctctaac agcggcacca gttctactgg cgtggcgtag taatgatatt
    19141 tcgcccgact acgcgcagtg gtggcgacag tgaaatcgac atcgtgtaac gattcagcca
    19201 gtgtcgggaa aactttaata ttatcaataa tatcactaga tccatgtgcg acccagcggg
    19261 tggctggctc caggtgtgcc tgactatcga caatccgcag ttcgctaaaa cccatcgttt
    19321 tcattgcccg cgccgctgcc ccaatatttt ctgctctggc gggtgcgacc aggataatcg
    19381 ttatacgcat attgccactc ttcttgacca aataaccgcg aacagggtga tcactgtcaa
    19441 cttattacgc ggtgcgaatt tacaaattct taacgtaagc cgcagaaaaa gccctttact
    19501 tagcttaaaa aaggctaaac tattttctga ctgcactaac ggtttaattg ttaaaaaatg
    19561 ctacatatcc ttctgtttac ttaggataat tttataaaaa ataaatctcg acaattggat
    19621 tcaccacgtt tattagttgt atgatgcaac tagttggatt attaaaataa tgtgacgaaa
    19681 gctagcattt agatacgatg atttcatcaa actgttaacg tgctacaatt gaacttgata
    19741 tatgtcaacg aagcgtagtt ttattgggtg tccggcccct cttagcctgt tatgttgctg
    19801 ttaaaatggt taggatgaca gccgtttttg acactgtcgg gtcctgaggg aaagtaccca
    19861 cgaccaagct aatgatgttg ttgacgttga tggaaagtgc atcaagaacg caattacgta
    19921 ctttagtcat gttacgccga tcatgttaat ttgcagcatg catcaggcag gtcagggact
    19981 tttgtacttc ctgtttcgat ttagttggca atttaggtag caaacatgca gaccccgcac
    20041 attcttatcg ttgaagacga gttggtaaca cgcaacacgt tgaaaagtat tttcgaagcg
    20101 gaaggctatg atgttttcga agcgacagat ggcgcggaaa tgcatcagat cctctctgaa
    20161 tatgacatca acctggtgat catggatatc aatctgccgg gtaagaacgg tcttctgtta
    20221 gcgcgtgaac tgcgcgagca ggcgaatgtt gcgttgatgt tcctgactgg ccgtgacaac
    20281 gaagtcgata aaattctcgg cctcgaaatc ggtgcagatg actacatcac caaaccgttc
    20341 aacccgcgtg aactgacgat tcgtgcacgc aacctgctgt cccgtaccat gaatctgggt
    20401 actgtcagcg aagaacgtcg tagcgttgaa agctacaagt tcaatggttg ggaactggac
    20461 atcaacagcc gttcgttgat cggccctgat ggcgagcagt acaagctgcc gcgcagcgag
    20521 ttccgcgcca tgcttcactt ctgtgaaaac ccaggcaaaa ttcagtctcg tgctgaactg
    20581 ctgaagaaaa tgaccggccg tgagctgaaa ccgcacgacc gtactgtaga cgtgacgatc
    20641 cgccgtattc gtaaacattt cgaatctacg ccggatacgc cggaaatcat cgccaccatt
    20701 cacggtgaag gttatcgctt ctgcggtgat ctggaagatt aatcggcttt accaccgtca
    20761 aaaaaacggc gctttttagc gccgttttta tttttcaacc ttatttccag atacgtaatt
    20821 catcgtccgt tgtaacttct ttactggctt tcattttcgg cagtgaaaac gcataccagt
    20881 cgatattacg agtcacaaac atcatgccgg ccagcgccac caccagcaca ctggttccca
    20941 acaacagcgc gctatcggca gagttgagca gtccccacat cactccatcc agcaacaaca
    21001 gcgcgagggt aaacaacatg ctattgcgcc agcctttcaa taccgcctgc aaataaatac
    21061 cgttcattaa cgccccaatc agactggcga ttatccatgc cacggtaaaa ccgatatgtt
    21121 cagaaagcgc cagcaagagc aaataaaaca tcaccaatga aagccccacc agcaaatatt
    21181 gcattgggtg taaacgttgc gcggtgagcg tttcaaaaac aaagaacgcc ataaaagtca
    21241 gtgcaatcag cagaatggcg tacttagtcg cccggtccgt taattggtat tgatcggctg
    21301 gcgtcgtcac tgcgacgcta aacgcgggga agttttccca gccggtatca ttgcctgaag
    21361 caaaacgctc accgagatta ttagcaaacc agctgctttg ccattgcgcc tgaaaacctg
    21421 actcgctagc ttcccgtttg gctggtagaa aatcacctaa aaaactggga tgcggccagt
    21481 tgctggttaa ggtcatttcg ctattacgcc cggcaggcac cacagaaaga tcgccggtac
    21541 cgcttaaatt cagtgccata ttcagctgca ggttctgctt ccgccagtcc ccttcgggta
    21601 aagggatatg cacgccctgc ccgccttgct ctaacccggt accgggttca atggtcagtg
    21661 ccgttccgtt aacttcaggc gctttcacca caccaatacc acgcgcatcc ccgacgctaa
    21721 tcacaataaa gggcttgcct aagatgatat ttggcgcgtt gagctcgcta agacgcgaaa
    21781 catcgaaatc ggcttttagc gttaaatcac tgtgccagac ctgaccggta taaatcccta
    21841 tcttgcgttc ttccacgttc tgattgccat caaccatcaa tgactcaggt aaccaaaaat
    21901 ggataaaact tcgtttccgc tccacggttt tatcctcttc ctgcaccgta taaagctcgg
    21961 tcacaggaat agcgataagc ggcccaacga gtttttgcgg cccgctggta ctttggcgaa
    22021 tcgcatcttc cacatcgcta cggtaatcgg cacgttcgac aatcacttgc cgaatcagca
    22081 ttatcggaat caacaacagc aatactgcac caaacaaggt ggtcattttc cagaacaggg
    22141 gggatttcaa catagtcttc tcctttgcaa tggcagcagg atacgaaggc tatgtgggaa
    22201 gaatttgaag ctatgtgaag ttacggtgaa gtcgtagcga ggccagcacg ccaccttcct
    22261 gcacgttgcg cagcgtgact tcgccgttaa acaaacgggc gacctcactg acgaacgcca
    22321 accccagacc gctgcttttt tgcccatttg cacgcggcaa agagtaaaag cgttcaaaaa
    22381 tacgtgacag cgcgtagtca ggaataccac taccggtatc cagcacctta agcgtgacgt
    22441 gttcctgatc cacttcggcg cttagcgtta tgcaaccgct ctcgggagta aaatcgatgg
    22501 tgttatccag taaattcccc agcgcctgat ccagtaacgc cggttcagca gcaacattaa
    22561 cctcggtggg cataacatgc aaagtgatgt ttttttctgc caactgcacg gtgcgcgctt
    22621 cgctgacgcg gcgaaataat gccgccacat caacagcagt cagaacgact tcctgacgat
    22681 tctccagtct tgcctggcgt agtaacgttt ccaccagcgc ctgcattcgc gcattttgtg
    22741 tcagaatgtt gtcagtaaaa cgagccacta cttcaggcgg cggaccttcg cgtaaaattt
    22801 ccgccgcgcc acgaatcgcc gccagtgggc tttttagctc atgggttaac gcataaacat
    22861 actgctcaat atagtttttc ccttccagct tcacgcgcat actttccagc gcctgcgcga
    22921 gtttacgcag ctcgctacta ccgagatcgg gaagaggaac gggcttattg tcagtgacgg
    22981 aatcagcata gcgagtgagc cgggcaatgg agcggttgat ccaccaaacc atgcctgcgc
    23041 caatcaccag tgcaatcccc aacaaaatgg cgctggccca taaaattcgc cgctcgctgc
    23101 gtttaatgac cggagccatc gccgcgttcg gtttgcctac gctcaaaacg ccaataagcc
    23161 gcgagccgcc cataatcggc gcggcaacat acatcaccga actttcggga tcggcaggat
    23221 tttgcaacgt gctgcgcgca ccatactgac cacgcaacgt tagccagacg tcattccagc
    23281 gcgaataatc ctgtccaacg gctttatttg ccgaatcgaa caatactttg ccatgcgcat
    23341 cggtcatata gacatgatat tcattgcgca ctttgttaat gccaccaata ttggcgcgaa
    23401 acgggcgatg ttgtagctga ttaaacgcct gcgccagttg cccatgcgtt gggtccccag
    23461 agagcaaatc gggacgcgcc agctccgcaa gtaacgttgc cgtgtcgatc aacgtcccct
    23521 ccgttgctct tcgcacgccc ggtttaactt ctttgacaaa aatagccagt acgaaccagg
    23581 ccgccaccgc caccagtaaa aaatagccca gcagcaaccg catgccgata cgcattacaa
    23641 gcccctcagg ctatatccca tgccgcgatg agtattaatc ggtgaaagat cagggttgat
    23701 ggcgcgcagc ttggcacgca gcgttttaat gtggatatcg acggtgcgat cgtaggtgtc
    23761 ctgcgcatct tcccacacgc tatccattag ttgctggcgg gaccagacgc ggcctggcga
    23821 cttgagtaac gtcttcagca ataaaaactc ataccgcgtc agcgttaatg gcgtgtcaaa
    23881 ccagctgatc tgcgccgcgg gttcattcaa ttcaaaatga ccaatacgga tgacgggaga
    23941 cggcgtcgag aacttcttca cccgacgcag taaggtgcgc accctggcgc acacttcgcg
    24001 gggtgaaaac ggtttagcca catagtcgtc aacgccaatt tccagcccaa gcaggcgatc
    24061 gacctcttca cttcgggccg tcaggaacag tacaggtaac gccggatgga gcgccagtaa
    24121 ctggcggcac aattcaaagc cactgatatc aggcaaacca acgtcgagaa tcatgacgtc
    24181 gggaacctgc tggcgagctt tatccagcac cggcaaacct cgctcaaaga cctcgacggc
    24241 aaaaccttcc tgctgcaaca tgtagaccag cgtgtcggct atcccttgct catcttccac
    24301 taaccagacc gtttcccgtt gcataaatcg cctctgttat tgccgccacg gcatgacagg
    24361 aaccgcgcta atcgcgtttt tcggcgaacc ttctacaact ttgtcggagt aagccagata
    24421 agcgagcgcg ttgcgtttgg cgtcataaaa gcgcacgacc tgtaacgact taaagaccag
    24481 ggacgtgcgt tttttgaata ctacctcgcc ctgagctttg ccgtttttaa tacgatccga
    24541 cagttcaatc ggcccgactt gctgacaaga aatggccgca tcggaggtat cttctgccag
    24601 gcccaatccc cctttaatac caccggtttt cgcccggctc acataacagg tgacattttt
    24661 cacatcggga tcgtcaaagg cttccacaac aattttgtga tccgggccga tcattttaaa
    24721 tacggtgtcg accgaaccaa tctcttctgc atgagccaat ggccccagca ttatcaggct
    24781 taaagacagg atcaaatgct tgtatttcat attgttacca tttttatcta ttacacgagc
    24841 aattagttcg tcacggtaaa taacatattg attaagaaca aaatctcaat tcttttattt
    24901 ccgtcaggcc ctaaaacata ctctactaag aaaaaaacac tgaatgctaa aacagcaaaa
    24961 aatgctatta tccaattacc tgatgtcagg tgctcgttgt tgaaaggatg aggatatttt
    25021 atggatcagg ccggcattat tcgcgacctt ttaatctggc tggaaggtca tctggatcag
    25081 cccctgtcgc tcgacaatgt agcggcgaaa gcaggttatt ccaagtggca cttacagaga
    25141 atgtttaaag atgtcactgg ccatgctatt ggcgcgtata ttcgtgctcg tcgtttgtcg
    25201 aaatcggcgg tcgcactacg cctgactgcg cgtccgattc tagacatcgc gctgcaatac
    25261 cgcttcgact ctcaacagac atttacccgc gcattcaaga agcagtttgc ccagactcct
    25321 gcactttacc gccgttctcc tgaatggagc gcctttggta ttcgcccgcc gctacgtctg
    25381 ggtaaattca caatgccaga gcacaaattt gtcaccctgg aagatacgcc gctgattggt
    25441 gttacccaga gctactcctg ttcgctggag caaatctctg atttccgcca tgaaatgcgt
    25501 tatcagttct ggcacgattt tctcggcaac gcgccgacca ttccgccggt gctctacggc
    25561 ctgaatgaaa cgcgtccgag tcaggataaa gacgacgaac aagaggtatt ctataccacc
    25621 gcgttagccc aggatcaggc agatggctat gtactgacgg ggcatccggt gatgctgcag
    25681 ggcggcgaat atgtgatgtt tacctatgaa ggtctgggaa ccggcgtgca ggagtttatc
    25741 ctgacggtat acggaacgtg catgccaatg ctcaatctga cgcgccgtaa aggtcaggat
    25801 attgagcgat actacccggc agaagacgcc aaagcgggag atcgcccaat taatctgcgc
    25861 tgtgaactgc tgattcctat ccgccgttaa cgctgcagct catctaatgc aggggcgtcc
    25921 agatgcgaga tgtcccctgc ggtttcaacg acccagccag acgccagcca caggctttcc
    25981 tgataatcca cgcgcgaaat agaacagtta cgcagacgta agcggcgctc tgcccatgct
    26041 ggtaatccga gaatcgtact caccagacat cccagtgcaa taccatggct caccagcagc
    26101 ggtcggcttc cctgcggtaa gtcccggcaa gattccagtg ccgcattgac gcgatcgctg
    26161 agctcctgca ttgactcgcc ttcaggaata cgcccgtcaa cggtgccatt gaccagctgc
    26221 cgacgccagt tctcttcttc ttcggtcaga gaatcgatat gtcttttttc cagcacaccc
    26281 atgtttaatt cacgcaggcg agaatcaaag atgatgtcac agccgcaggc ctgggcgatg
    26341 atttccgccg tacgccgggt gcgtcctaaa tcgctactga taatatgcgt aatgccaagc
    26401 tctttggcac gggttgccac ctgcatcgcc tgttgctcac ctttggcggt cagcgggctg
    26461 tcagactggc cctgaatacg tcgctcggcg ttccactgcg tttcaccgtg gcggactagg
    26521 tatacctgta acatgctttt tttccgtata ctgctgtaac gttaattttc tgagtaatgc
    26581 tgattatgca ccaagttgtc tgtgcgacca ccaatcccgc taaaattcag gccattctgc
    26641 aggcatttca cgagatcttc ggcgaaggat cctgccatat tgcatccgtt gccgtcgaga
    26701 gcggtgtacc ggaacaaccc tttggcagtg aggaaacgcg cgctggcgca cgaaatcggg
    26761 tagcaaatgc ccgccgttta cttccagagg ctgatttttg ggtggcgatt gaagctggca
    26821 ttgatggcga cagcactttc agttgggtgg tgattgaaaa cgccagccag cgcggcgagg
    26881 cgcgttcggc aactttgccg ttacctgcgg tgattctgga aaaagttcgt gaaggcgagg
    26941 cgcttggtcc cgtgatgtcg cgttataccg gtattgatga gattggtcgt aaggaagggg
    27001 cgatcggcgt gtttaccgcc gggaaactca ctcgcgccag cgtctatcat caggcggtga
    27061 tcctggcgct gagtccgttt cacaatgccg tgtatcaggc attgtaggac ggataaggcg
    27121 ttcacgccgc atccgacatt cggtgcacga tgcctgatgc gacgctgccg cgtcttatca
    27181 ggcctacaaa atcaatcgct tttcagcaac acctcttcca gccactgacg cagctccacg
    27241 ggcgcggctt tcaggctgtt agatccacgc gtaatcgtcg cgatgcccgc gccgagttca
    27301 ttttttaact cacgctgact catttcgcca cgcaacagct cttcgacaat acgcacgcga
    27361 gtccccaacg cttcgcgctc atctggcgtc agcatcaggt ttaacaacgg taaatggaga
    27421 tcgttttggt aggcattctt aagcaggtcg acaaaacgta accactcctg gtgacgctgt
    27481 tctgccatcg ctgctgaata gggtgattgt tgggccataa tatgtcgcca ttgttagcgg
    27541 gggaagcaaa atgactcccc cggttgtact cgctaaagag tacgatagca tatcataaac
    27601 gtgcggatca gtaacgacgt ccccattccg tggcgctcat caacgtcggt ttatccccca
    27661 tgaaatagcg gtagtaagca tcataagcca gcacgttctt cacataaccg cgcgtctcgg
    27721 agaacggaat actctcgaca aatgccacag cgtcgatacg cccggcgctg ttgccaagcc
    27781 aagttcgcac ccgtccaggt ccggcgttat aagctgctga ggagaaaata cggttattgc
    27841 caaactgctg ataaacatat tgcaggtaac tggtgccaat gttgatgttc gtttccggat
    27901 ccagcaattg cccagggctg ctataaccag gaatagagaa catcttcacc gtatgggtcg
    27961 ctgtaccagg cataatctgc atcaggccgc tggcccctac cggtgatttc actttcggat
    28021 tccaggcgct ctcctgacgg gcaatcgcca tcgcatagct ttgcgggatc tctttcccac
    28081 tggtgtaacg tttgaaaaga tcgttgtaag ccagcgggaa tcgctcttcc agatgatccc
    28141 aaagcttccc ggcgatcgtt gcctgaacgc taagatccca ccattggtta ttgaaagcat
    28201 accgagccag ttgagcctgc tctgtttttg acttgctctt caccagattg gcccactcgc
    28261 tacgcgcggt attatcgaga ttccagtaca tcaactcgcg cacgcgcgcc atctccggcc
    28321 cctgagtcag ggtgctgtca acattctgcg gcgctttatc aatcttcagc tcatactctt
    28381 caccgatgcg ttgtgctgca accatcgggt agaaaccacg ctgttgcatg agttgatgca
    28441 aaatctcttt cgcttcagct tcacgtccgc gttccagcaa taaatccgcc tgccagtaac
    28501 gccattcatc tttctctttt gcttccatcg gcagacgcgc cagccaggta ttcaggccgc
    28561 gacgatcgcc ggtgccaagc gccattcgca cacggcgttc aatcaacgac gtggattgcg
    28621 agcgcataat ggcgtcatcg cgccatttcg cctgctcatc ggtgacatca ttgcccatca
    28681 aacgccaggc gacgatatcg cgcagctcct gaatctgatc ttcattaagc tgctgcgcct
    28741 gggcaagcga cgggatcatc agccgtgcat tttcggcatc ctgccgcgcc acactggcaa
    28801 acgccaccgc cgccatttga tgggtaaaat cggtcgcgcc ggttgtacgc gcgaaggtca
    28861 gtaccgtatt agggttgttc gccagtgaaa tgattgccga ggcgatagtc tggtaatcgg
    28921 caggcatctg ccctgccagc actgttacca ggcctgtgtt acccgctttc atcgccagac
    28981 ggatacgctc taaatacgcc agcggatctt gtttacctga cgcacgccag acgctaaata
    29041 acttgtcaca ggcgttaggc tggctcttgc cggttagcca cagctctttc gccccttgcc
    29101 aggcttcttc actctgcccg gtgttccatt tcgcatagta gtaattacat tgcgcttcgg
    29161 tagttccggg cttttccggg ctaaaggcta acaagccacg ccagtcttca cgccgcgcca
    29221 gttcattgac gaaacgagat tgcagcgtgc gagcgggagg aagcgtgggg ttagcgcgaa
    29281 caaagttagt gaccgtcacc gccggttgat tcatcaggtc atcggtgatc tggcggtatt
    29341 ccaggtaggg ataaagcgga taatccttca gtccaggcat catttgttcg accacatcca
    29401 tctgtcgatt atcccaggcc tgcttgattt gcgcgtaacg gctacgctgc tcatccagtg
    29461 agtcggctcg cgccacgctg ctgaccgtca gcagacagac accggcagcc aacagccgcc
    29521 aggtaacttg tttggctttt tccacaagcg catcctctaa gtgtaaatac atcaatgcag
    29581 cgtcataccg cgtaatagat ttatgctaac cagtcattgc cgtttacgcc acgttacgga
    29641 cactttttta cttttactgc gaaggtgatc gaccaactgg gattaggcgg ggaaacaggc
    29701 tacacttcgc ctttgaaaac tcatcatcac gataaacata gaggcgaagt ccaacgtggc
    29761 tcaattcgtt tataccatgc atcgtgtcgg caaagttgtt ccgccgaaac gtcatatttt
    29821 gaaaaacatc tctctgagtt tcttccctgg ggcaaaaatt ggtgtcctgg gtctgaacgg
    29881 cgcgggtaag tccaccctgc tgcgcattat ggcgggcatt gataaagaca tcgaaggtga
    29941 agcgcgtccg cagccagaca tcaagattgg ttatctgccg caggaaccgc agctgaaccc
    30001 ggaacacacc gtgcgtgagt ccattgaaga agcggtttca gaagtggtta acgccctgaa
    30061 acgcctggat gaagtgtatg cgctgtacgc cgatccggat gccgattttg acaagctggc
    30121 cgctgaacaa ggccgtctgg aagagatcat tcaggctcac gacggtcata acctgaacgt
    30181 acagctggag cgtgcggcgg atgcgctgcg tctgccagat tgggacgcga aaatcgctaa
    30241 cctctccggt ggtgagcgtc gtcgcgtggc gttgtgccgc ctgctgctgg aaaaaccaga
    30301 catgctgctg ctcgacgaac cgaccaacca cctggatgcc gaatccgtgg cgtggctgga
    30361 acgcttcctg cacgacttcg aaggcaccgt ggtggcgatt actcacgacc gttacttcct
    30421 cgataacgtt gcaggctgga tcctcgaact ggaccgcggt gaaggtattc cgtgggaagg
    30481 taactactcc tcctggctgg atcagaaaga tcagcgtctg gcgcaggaag cttcacaaga
    30541 agcggcgcgt cgtaagtcga tcgagaaaga gctggagtgg gtacgtcagg gaactaaagg
    30601 cagccagtcg aaaggtaaag cacgtctggc acgctttgaa gagctgaaca gcaccgaata
    30661 ccagaaacgt aacgaaacca acgaactgtt tattccacct ggaccgcgtc tgggcgataa
    30721 agtgctggaa gtcagcaacc tgcgtaaatc ctacggcgat cgcctgctga ttgatgacct
    30781 gagcttctcg atcccgaaag gggcgatcgt cgggatcatc ggtccgaacg gcgcgggtaa
    30841 atcgaccctg ttccgtatga tctccggtca ggaacagccg gacagcggca ccatcacttt
    30901 gggtgaaacg gtgaaactgg catcggttga tcagttccgt gactcaatgg ataacagcaa
    30961 aaccgtttgg gaagaagttt ccggcgggct ggatattatg aagatcggca acaccgagat
    31021 gccaagccgc gcctacgttg gccgctttaa ctttaaaggg gttgatcagg gtaaacgtgt
    31081 tggcgaactt tccggtggtg agcgcggtcg tctgcatctg gcgaagctgc tgcaggttgg
    31141 cggcaacatg ctgctgctcg acgaaccaac caacgacctg gatatcgaaa ccctgcgcgc
    31201 gctggaaaac gccctgctgg agttcccggg ctgtgcgatg gttatctcgc acgaccgttg
    31261 gttcctcgac cgtatcgcca cgcacatcct ggactaccag gatgaaggta aagttgagtt
    31321 cttcgaaggt aactttactg agtacgaaga gtacaaaaaa cgcacgctgg gcgcagacgc
    31381 gctggagccg aagcgtatca agtacaagcg tattgcgaag taatgcgtaa aatgccggat
    31441 gcggcgcgaa cgccttatcc ggcctacaaa accagcaatt tcaatacgtt gtaaaactgt
    31501 aggcctgata agacgcggta agcgtcgcat caggcattga gcaccgaatg ccggatgcgg
    31561 cgtgaacacc ttttccggtc tacaacagcc ttagcatcct caatttcgtg gcgggtcacc
    31621 cgtgcaggtt ttttcaaacc ttgtttaatc tcgacagcct cttccagaga ggttttcaac
    31681 tcatcaaaaa aactcatttt atacctcgcc tttgagcagt ttcgtcacgg ttatccttgc
    31741 tcccccatca tctcccgcac cagctccacg cagcgcagga aacggctgtc gtaatcgtcc
    31801 tcttcaaccc gcacgaattc gatattgttc tcttccagca tctccaccag caagttctgg
    31861 aactctttgc gatccaccga actgccgagg ctgcgtaaac catccgccac ccacggcgtg
    31921 ttgttctcca gcaggatcac cagatcgaaa cggtattcat caatcagcgc ctgcacgaac
    31981 ggatgctcac gcccttcgta ctttttgcag aacgcctggg tagtgacaaa atcggtgtca
    32041 ataaacgcca ctttattggc gtatttcact gcaaaatcaa tgtattgtgc gtggcccaac
    32101 gcgattttat cgtagtcgga atactgcaat gcgatctcat caccgccgag gtgtgaaaag
    32161 acataatcgc ggccatattc ccacgcactg gtggtgttga agatattggc aagtttgttt
    32221 accagggtgg atttaccgct cgactcgccg ccaaggatcg ccacggtacg cacaaaaaac
    32281 ggcttcactt cggtaggaat atattcccag tagcggaacg ggttttcgcg gatctgcgca
    32341 ccgctgatac tcataaaggt acgtttcgga tcaaccagca ccgtctcgat ccccagatgt
    32401 tccatatact gtggcgcatc ggcttcttcc gaggtgtaga tcaggtccgg ctggatcccc
    32461 ttttcagcca taaacttttt gatgccgttg ctccacacat cccagccatg cggatacggc
    32521 tccatgccct cttcgttgaa agcatgaata cggatatttt tctgatattt aaaggtttgc
    32581 aatagccagc gcagacgatc cggcaccgtg ggctgctgcg acatggcgct gtcttcaaac
    32641 aacgcgcggt cgcgggtatc gtcaaaaccc ataatgatat gcagctcgtc aacctggcta
    32701 caggcgcgct ggataaggta gatatgtccg gtatgcagcg ggtagaactt accgaatacg
    32761 acaccaatgg ttttcttctg ccggggaaat tcaagcccca aaaaacggtg caatgcctcc
    32821 agcttttgcg cgctggggct tttaattttg gcattcagta actggcttaa ataccctttg
    32881 gtcataccgc tggcatcagc tacctgctgt agcgtgcagc cctgttgctt gatggcagtt
    32941 ttcaggtaat caaatgacga catatctccc tccgtatctc tcattataag tcgtcgaaca
    33001 cgctaagcgc gtcggagagt tttttaacgc caaaaatctg catcccttcc ggcgcttttt
    33061 tcggtacgtt agccgccgga acaatcgccc ggcgaaaacc gtgtttcgcc gcttcagaaa
    33121 tccgctcctg gccactgggc accgggcgga tctcccctgc cagcccgact tcaccaaaca
    33181 ccaccagatc ctgtggcagc ggtctgtcac gcaggctgga aaccatcgcc agcagtaacg
    33241 ctaagtcggc gctggtttcg gttaccttca cgccgccgac cacgttcaca aacacatcct
    33301 gatcggccat ttgcagacca ccgtgacggt gcaagacagc cagcaggatt gccagacggt
    33361 tttgttccag ccccactgcc acgcggcgcg gattcgccat tatcgagtga tcgaccagcg
    33421 cctgaatctc caccagcaac ggacgcgttc cttcccacac caccatcacg gagctgccgg
    33481 aagtcacttc atctccgcga cttaagaaaa ttgccgaagg gttgctgact tcacgcagcc
    33541 cctgttcggt catcgcgaag acgcccagct cattcaccgc gccgaagcgg tttttatggc
    33601 tgcgtaaggt gcgaaaacgg gagtcggcat cgccatccag aagcaccgaa cagtcgatac
    33661 agtgttccag cactttcggg ccagccagcg aaccatcttt ggttacgtgt ccaaccatga
    33721 caatcgccac accgcgcgtt ttggcgaaac gcgtcagata agccgccgtt tcacgcacct
    33781 gcgccacgct accaggcgat gattgtacat ccgccatatg catcacctgg atcgagtcaa
    33841 ttaccatcag cttcggttgc tcttcttcgg caatcaggca gatctgctcg atgctggttt
    33901 ccgacaacat attgagattg tcagtcggca ggccaaggcg atgagcgcgc attgccacct
    33961 gttgcagcga ctcttcgccg gtgacataca gcgttttcat ctgctgggcc agtttgcaca
    34021 gcgtttgcag caacagcgtg gatttccccg caccagggtt accgccaatc agaatggcac
    34081 ttcctggcac cacgccgccg cctagtacgc ggtcgaactc tttaaatccg gtggaaaaac
    34141 gcggcagctc ttcaaggctg atatcggaga gtttctggac ttttgccacc ccggcgctac
    34201 cggcatagcc gctgagacgc tcgttacgcg ccaccgttgg cgacgcagca agacgcacct
    34261 cggtgatggt gttccaggca tgacaggcac tgcactgccc ctgccagcgc ggataatcgg
    34321 ccccgcattc attacaaaca aaggcgcgtt ttggagcttt tgccacggtg tacctcgtta
    34381 atgctgtgcc gcccgcagga tgacgggcag gcaattactt ctgattcagg ctgccagaga
    34441 ggatgcagaa tacccccatc aggtcagcgt gacggatggt gacttccgcc ttttcattca
    34501 cttttggctt ggcatggtag gcaatcccca gccctgctgc tttgatcatc ggcaggtcat
    34561 tagctccatc gccaatcgcc acggtctgcg ccagcgggat ttcatactcc tgcgcgaggc
    34621 gagtcagagt tttcgctttg tactgtgcgt ctacgatgtc gccgatcaca ttgccggtaa
    34681 atttaccgtc catgatctcc agttcattgg ctagcacggc tgtcaggcgc agcttgtcgc
    34741 gcaggtattc agcaaagaaa gtaaagccgc cggaggcaat cgccactttc cagcccagcg
    34801 tttccagctt gagcaccagc tgcgttaagc ctggcatcag cggcagattt tcacgcacct
    34861 gttgcagaat attggcgtca gcgcctttca gcgtcgccac gcggctgcgc aggctggcgg
    34921 taaaatcgag ttcgccgcgc atcgcccgtt cggttacctc cgctaccatc tcgcccgttc
    34981 cggccagctt ggcaatttca tcaatacatt caatctggat ggcggtggaa tccatgtcca
    35041 tcaccagcaa acccggcgtg cgcaggtgcg ggattttccc cagcggggcg acatccagct
    35101 gcgcttcgtg ggccaggcgt gtagcccgtg cggtgagtga acctgccaga cgaatcacct
    35161 gataatcttc cacgcaccag gcggcaacaa tcaccatcgc cgcacccagt ttgctctggt
    35221 attgggtcag acgctgttta tccagcccac gaccatacag cagccagccg ctacgagctg
    35281 cgtggtaatc cagtggcatc acttcatcac cacttaatga aagaggcaga cccggccata
    35341 aagagacatc ttcaggcagg tcgcaccagg taatgttagg cattaaggct cctgtaaaat
    35401 cgttcgaagc agggaaaata acgcatgagg ctaccttgta tccattgctt ctggcaacat
    35461 taagtctcaa attttcaaag ggtggaagat ggctcgcaca aaactgaaat tccggctaca
    35521 tcgggcagtg attgtcctgt tctgtcttgc cttgttagtg gcactgatgc agggagcgtc
    35581 atggtttagt caaaaccacc agcgacagcg taatccacag ctggaagaac tggcccgcac
    35641 cctggcgcgt caggtgacgc tgaacgttgc accgctgatg cgtaccgact caccggatga
    35701 aaaacgcatt caggcgatcc tcgatcagtt aacggatgaa agccgtatcc tcgacgcggg
    35761 tgtgtatgac gaacaaggcg atcttatcgc acgttctggc gaaagcgtcg aagtgcgcga
    35821 tcggctggcg ctcgacggta aaaaagcagg cggctatttt aaccagcaga ttgtcgagcc
    35881 aattgcgggt aaaaacggac cgctcggcta tctgcgcctg acactcgaca cccatacgct
    35941 cgccaccgaa gcccaacagg tggataacac cactaacatt ttacgcctga tgttgctgct
    36001 ctcactggca atcggcgtag tgctgacccg cacgcagcta cagggtaaac gcacccgctg
    36061 gcagcaatcg cccttcctgt taaccgccag caaaccggtg ccggaagagg aagaaagcga
    36121 gaaaaaagag tgacccatta ctacaagaaa ggaaatcgtt atgtccacat tacgcctgct
    36181 catctctgac tcttacgacc cgtggtttaa cctggcggtg gaagagtgta tttttcgcca
    36241 aatgcccgcc acgcagcgcg ttctgtttct ctggcgcaat gccgacacgg tagtaattgg
    36301 tcgcgcgcag aacccgtgga aagagtgtaa tacctggcgg atggaagaag ataacgtccg
    36361 cctggcgcgg cgcagtagcg gtggcggcgc ggtgttccac gatctcggca atacctgctt
    36421 tacctttatg gctggcaagc cggagtacga taaaactatc tccacgtcga ttgtgctcaa
    36481 tgcgctgaac gcgctcggcg tcagcgccga agcgtccgga cgtaacgatc tggtggtgaa
    36541 aaccgtcgaa ggcgaccgca aagtctcagg ctcggcctat cgcgaaacca aagatcgcgg
    36601 cttccaccac ggcaccttgc tactcaatgc cgacctcagc cgcctggcaa actatctcaa
    36661 tccggataaa aagaaactgg cggcgaaagg cattacttca gtgcgttccc gcgtgaccaa
    36721 cctcaccgag ctgctgccgg ggctcaccca tgagcaggtt tgcgaggcca taaccgaggc
    36781 ctttttcgcc cattatggcg agcacgtgga agcggaaatc atctccccgg acaaaacgcc
    36841 agacttgcca aacttcgccg aaacctttgc ccgtcagagt agctgagagt ggaacttcgg
    36901 tcaggctccg gcattctcgc atctgctgga tgaacgcttt agctggggcg gcgtggaact
    36961 gcatttcgac gttgaaaaag gccatatcac ccgcgcccag gtatttaccg acagcctcaa
    37021 ccccgcgccg ctggaagccc tcgccgggcg actgcaaggc tgcctgtacc gcgcggatat
    37081 gctgcaacaa gagtgcgaag cgctgttggt tgacttcccg gaccaggaaa aagagctacg
    37141 ggagttgtcg acgtggatag cgggggcggt aaggtaactc gctgattaaa atatccaagg
    37201 aaggaaatat gatcgatctg gaaaaccagg aacgggagat tatcaattta atgttcagcc
    37261 agcgtatttc ctggctagcc gccgtgcgta tcaggcataa attatcgctt gctgaagtaa
    37321 gtaaaatgtt aggtatctcg attaattctc taaaacagat cgaaaagacg gaacggctta
    37381 gctcaaacat caaaagcaaa atggcagaaa tttatggttg tccaccagag ttgctgatat
    37441 gcccttcctg gatgacggct gaacataaat gaatagattc gtgaatagat acttgaatgt
    37501 attttatttt ctatccatat gaaaaataat agtaaatcta taaatcagtt gctattgatg
    37561 aatagatact aaaaagccgg agcagtcccc cactccggct taacacgaca atcgcctttc
    37621 agcgaaacac aattactctt tatcgcccag cagaacggat tccagtgcga ttttgatcat
    37681 gtcgttgaag gtggtctgac gctcagcggc agtggtctgc tcgtgagtgc ggatgtggtc
    37741 agacacggtg cagatggtca gggctttcgc gccaaactct gcagccacgc cgtagatacc
    37801 agccgcttcc atttccacgc cgaggatgcc gtatttttcc atcacgtcga acatttcgcc
    37861 gtccggagag tagaacaggt cagcggagaa caggttaccc acgcgagcat caacgcccag
    37921 tgctttagct gcatctactg cgttacgcac catgtcaaag tcagcgatag cggcaaagtc
    37981 atggtcttta aaacggatgc ggttaacttt ggaatcggtg caggccccca taccgataac
    38041 gacgtcgcgc agttttacgt gcggcagaac tgcgccacag gaaccaacgc ggataatttt
    38101 cttcacgccg aaatcggtga tcagttcttt ggtgtagatg gagcaggacg ggatacccat
    38161 accgtgaccc attacggaaa ttttgcggcc tttgtaagta ccagtgaagc ccagcatacc
    38221 gcgaacgttg ttcacttcac gggcatcttc aaggaaagtt tcagcaatat acttcgcacg
    38281 cagcgggtcg cctggcatca aaactacgtc agcgaaatcg cccatttctg cattaatgtg
    38341 tggggtagcc attgttttat cctttttgtg acataacaaa ggcggagtca acgctccgcc
    38401 caaatccatc agaacatggc tttgccatat tccatatcag aagtaccaaa atattttgcc
    38461 agagtctggc cgatatccgc gaaggtttca cggtgaccca gtgagcccgg ttttactttc
    38521 gggccatata ccagtaccgg aatgtgttca cgcgtgtggt cagtaccggt ccaggtcgga
    38581 tcgcaaccgt ggtcagcggt gaggatcagg atgtcgtcat cgcgcagcag agacatcagc
    38641 tccggcagac ggcggtcgaa cagttccaga cccgcagcat aaccggcgac gtcgcgacgg
    38701 tggccccagg aagagtcgaa gtcaacgaag ttggtgaaga cgatggtgtt atcacccgct
    38761 tctttcatct ctttgatggt ggcgtcaaac agcgcgtcca agccagtcgc tttcactttt
    38821 ttggtgatac cgcagttggc gtagatgtct gcaattttac cgacagaaac cacctggccg
    38881 tgtttttcat caaccagttt ctgcagcacg gtcggtgctg gcggctcaac agccaggtcg
    38941 tgacggttac cggtacgctg gaagttacca gctttgtcac cgataaacgg acgagcgata
    39001 acacgaccga tattgtagcc gccgttggtc agctcttcac gggcgatttc gcacagttcg
    39061 tagagtttat ccagaccgaa agtttcttca tggcaggcaa tctggaacac ggagtcagcg
    39121 gaggtataga aaatcggctt gccggttttc atgtgctctt cgcccagttg atccagaatg
    39181 accgtaccgg aagagtggca gttaccgagg taacccggca gattagcgcg ttcgaccagt
    39241 ttatccagca gctcttgcgg gaagctgttt tcgtgatcgg agaaatatcc ccactcaaac
    39301 agaaccggga caccggcgat ttcccagtga ccagacgggg tatctttacc ggatgacatt
    39361 tcgtgcgccc atgcgtacgc gccgataact tcagcgttgc cgtccattcc cgccggaatg
    39421 aaaccggtag aaccttcgtg tgctttcgcc agccccagac ggatcagatt tggcagattg
    39481 agcggccctt tacgaccatt atcagcttcg cctttggcac aagcttctgc gatatgaccc
    39541 agggtgtcag ccccgacgtc accaaagcgt tctgcatctt ctgtagcgcc gatgccgaat
    39601 gagtccagca ccataataaa tgcacgtttc atatgttctc cgtactcagt gcttcaaatt
    39661 taaatgatca gatcagtata ccgttattcg ctgatacggc gatagacagt tggtgtgctt
    39721 tccggtgcgt tatcggcaag tttaattgcc gctttcaccg ctttcgccgc ttcctgccag
    39781 ctgttttcgt ctttcgcgag gataaccgcc agcggacgct gaccgtctac ctggtcgccc
    39841 agacgcgcca tatcagtaaa gccgacgctg taatcgatgg tgtcagatgc ctgacgacga
    39901 ccgccgccca ttgcaaccac tgccatcccc agcgcgcggg tatccatttc actgacaaaa
    39961 ccttcggtat cagcatagac tgctttcgtc agcatcgctg tcggcagata cttcgcgtag
    40021 ttctcaacga agtcggtcgg gcctttttgt gccgctacca tacgaccaaa gacttctgcc
    40081 gctttaccgt tgtccagcac cgcctgcaat ttcgcgcgcg cttcggcgtc atctttcgcc
    40141 agtttgccgg agatcagcat ctccacgcac agcaccatcg tgacatcaaa cagacgcggg
    40201 ttacgatact cacccgtcag gaactgcacc gcttcacgaa cttcaaccgc gttacctgca
    40261 ctggaggcca gtacctgatt catgtcggtg agcagcgcgg tggtgcgcac gccagcgccg
    40321 ttagccacgc caacaatcgc ttcggcaagg gcttcagaga gttcgtaggt cggcataaac
    40381 gcgccgctac ccactttcac gtccatcacc agcgcgtcca gaccttccgc cagtttcttc
    40441 gccaggatcg aggcagtgat cagcgggatg gagtccacgg ttgcggtaat atcacgggtc
    40501 gcgtagaaac gtttatcagc cggagccagt gaactggtct gaccgataat cgccacgccg
    40561 acgtctttaa taatttcgcg gaaacggttg tcatccggga aaatgtcgaa gccagggatg
    40621 gattccagtt tgtcgagcgt accgccagta tgaccgaggc cgcgaccgga gatcatcgga
    40681 atatagccgc cgcaggctgc gaccatcggc cccaacatca gcgaagtgac atcgccgacg
    40741 ccgccggtcg agtgtttatc aacaatcggg ccattcagat gcaggctttt ccagtcgaga
    40801 acggttcctg aatctcgcat cgccatggtc agcgagacac gctcaggcat tgtcatatcg
    40861 tggaagaaaa tggtcatcgc gagggcggca atctgccctt cggaaatagt gttgtcgcga
    40921 ataccgttga taaagaaacg aatttcttca tcgctcagcg catgaccatc acgtttttta
    40981 cgaataattt cttgtgcgag aaacaaggta acctcccgag gaaaagagta tgaggcggct
    41041 tttgccggat gcgtctacgc cttatccggc ctacgggtag atacgtctcg taggcctgat
    41101 aagcgcagcg catcaggcgt aaagcatctt acttagtagc tgctggcgct cttaccgtcg
    41161 ccgtggccca gcgctttcag caggcttgcc agcaggctgg aagcaccaaa gcggtagtga
    41221 cgcgcatcag cccagtcagc accgaacagt tcatctgcga tggcgagata tttctgcgca
    41281 tcttccgcag tacgcacgcc gcccgccggt ttgaaaccaa cggttttttc tacgcccata
    41341 taacggatca cttccatcat gatgcgagcg ctttccggcg tcgcgttcac agccacttta
    41401 ccggtagagg ttttgatgaa gtccgcaccc gctttgatgg agatttcaga cgctttacgg
    41461 atcagcgctt cgtctttcag ttcgccggtt tcgatgatca ctttcagcag tacattcgct
    41521 gccgcgcaag cctctttaca ggctttcacc aggtcaaaac caacctgctc gtcacccgcc
    41581 atcagcgcgc ggtacgggaa caccacgtca acttcatcag caccgtaggc gattgccgca
    41641 cgggtttctg ccagcgcgat ttcgatgtcg tcgttaccgt gtgggaagtt ggttaccgta
    41701 gcgatacgga tttccggggt gccctgctct ttcagagttt tgcgagcaat cgggataaaa
    41761 cgaggataga tacagatagc ggcggtattg ccgaccggag ttttggcctg atgacacaga
    41821 gcaattactt tctcgtcggt gtcgtcgtca ttcagggtgg tcaggtccat caatttcagt
    41881 gcacgcaggc tgcttgcttt cagatcagtc atttcattct cctggcttgt cgccaataaa
    41941 attcaccttg cgagtttgtt agtattctaa catctactcc gcaacacact tcgatacaca
    42001 tcacaattaa ggaaatctac ttacaagttt gcatcactgt aatgcgatct ggttcaaata
    42061 attcactttc aaatgaatgc gtcagtggtg gcaaacgcat caggatcaaa gtgaacatca
    42121 cgaaacttct tacaatggcg catcaccttc ggcataaaag gaaaagaaga tgcccacttc
    42181 tcatgaaaat gcactgcaac aacgttgcca gcaaattgtc accagcccag tgcttagccc
    42241 ggagcagaag cgccattttc tggcactgga agcagaaaac aatctgcctt acccacagct
    42301 tcctgccgaa gcccgccgcg cgctggatga aggtgtaatc tgcgatatgt ttgaaggtca
    42361 tgcgccgtac aaaccgcgct atgtcttacc cgattacgct cgttttctgg cgaacggttc
    42421 cgaatggctg gagctggaag gcgcgaaaga tcttgatgac gcactctctc tgctgaccat
    42481 tctttaccac cacgtaccgt cggtcacatc gatgccggtc tacctggggc aactggatgc
    42541 gttgttgcaa ccgtatgtta gaattctaac acaaaacgag atcgatgttc gaataaaacg
    42601 tttctggcgt tacctcgaca gaaccctgcc agatgccttt atgcacgcca atatcggccc
    42661 gtctgattcg cccattaccc gcgcgatctt acgtgcagac gcgcaggttt caccgaacct
    42721 gacctttatc tacgatcctg aaatcacccc tgatgacctg ctgctggaag tggcgaagaa
    42781 catctgtgaa tgtagcaaac cgcacatcgc caacggtccg gtgcatgata aaattttcac
    42841 aaaagggggc tacgggattg tgagctgtta caactcactg ccgctggcgg gtggtggcag
    42901 cacgctggta cgccttaacc tgaaagccat tgccgagcgc agcgaatcgc tggatgactt
    42961 ctttacgcgc actctaccgc actactgcca gcagcagatc gccatcatcg atgcgcggtg
    43021 tgaattcctc tatcaacaat cacacttctt tgagaatagc ttcctggtga aagaagggct
    43081 gattaaccct gaacgttttg tgccaatgtt tggcatgtat gggctggcgg aagcggttaa
    43141 cttgctgtgt gagaaagaag ggattgccgc gcgctacggt aaagaagccg ccgcaaatga
    43201 agtgggttat cgcatcagcg cgcaactggc ggagtttgtc gccaataccc ccgtgaaata
    43261 tggctggcaa aaacgcgcca tgttacacgc acagtcgggg atcagttccg atatcggcac
    43321 cacgccgggc gcgcgtttgc cgtatggcga tgagccagat ccgatcaccc atctgcaaac
    43381 tgtcgccccg catcatgctt attattattc cggcatcagc gacattctga cgctcgacga
    43441 aaccatcaaa cgtaacccgc aggcactggt acagctttgc ctcggtgcct ttaaagccgg
    43501 aatgcgtgaa tttaccgcca atgtcagcgg taacgatctg gttcgcgtta ccggttatat
    43561 ggtgcgtttg tcggatttag aaaaatatcg cgccgaaggt tcacgcacca acaccacctg
    43621 gctgggcgaa gaagccgcac gcaacactcg tattctggaa cgccagccgc gcgtgataag
    43681 ccatgaacag cagatgcgct ttagtcagta agattatccc cttctcctgc gttgacgggc
    43741 caggcagtcg tctggctctg tttttgcagg gctgcaatct gcgctgcaaa aactgtcaca
    43801 atccctggac gatgggacgt tgcaatgact gtggggagtg cgtgccacag tgtccgcatc
    43861 aggcgttgca gattgttgac ggcaaagtgg tgtggaacgc tgtggtctgc gagcaatgtg
    43921 atacctgcct gaagatgtgt ccgcaacatg ccacaccaat ggcgcaatcc atgagcgtgg
    43981 acgaagtgct tagccatgtg cgcaaagcgg tgctgtttat tgaagggata acggtaagcg
    44041 gcggcgaagt cacgacccaa ctgccgtttg tagtggcgct gtttactgct atcaaaaacg
    44101 atccgcaact gcgccatctc acctgtctgg tggacagtaa cggtatgttg agcgaaaccg
    44161 gctgggaaaa attactcccg gtgtgtgacg gcgcaatgct cgatctcaaa gcatggggta
    44221 gcgaatgtca tcaacaatta accggacgcg ataatcaaca gattaagcgc agcatctatt
    44281 tgctggcaga gcgcggcaag ctggcggaac tgcgtttgct gatgatccca ggccaggtgg
    44341 attatttgca acacatcgaa gaactggcgg cgtttatcaa gggacttgga gatgttccgg
    44401 ttcgcctgaa cgcgtttcat gcccacggcg tgtatggcga ggcgcaaagc tgggcgagcg
    44461 ccacgccgga agacgttgag ccgttggctg atgcattaaa ggtgcgcggg gtgagccggt
    44521 tgatatttcc ggcgctctat ttgtgacatg ccggatacgg cctacggttc ggcacagact
    44581 tgtaggcctg ataagacgcg tcaagcgtcg catcaggcat tgtgcgccaa ctgccggatg
    44641 cggcgtgaac gccttatccg gcctacggtt cggcacaaac ctgtaggcct gataagacgc
    44701 gttaagcgtc gcatcaggca ttatgcgccg actgccggat gctgcctacg aagggcaaac
    44761 tacccacgaa cgttaaacac cgcatacgtg ttattaagca acacttcggc aatctcatcc
    44821 gcgggttccg ggcgtaactc gcaaagcacg gcaaacacac gggcagcctg ttccgggcgg
    44881 ttaggctgcc cctgaaaacc gttgagcggc atatccggcg catcggtttc cagcaacaac
    44941 gatgccagcg gtaattttgc gatgacatcg cgggttttac tggcgcgtgg ataggtgata
    45001 gtaccgccta cgccgatttt gtagcccagc tgtacaaacc gttcagcctg ttgctggctg
    45061 ccagaaaatc cgtggacaac accagcacgc ggcaaatcgt ggcgtttaag atgcatcgcc
    45121 agtttgtcgt gcgtgcgccg tgaatgcagg atcaccggca gatcgtagcg tttcgccagt
    45181 ttcagttgtt cgtcgagtaa ccactgctgc ctctcaaatt gcggatcgtc gccgaagaga
    45241 tccagaccga tctcccccac cgccaccacc ttcgccggac gcctttccag cgcctgctgt
    45301 agctgctcaa gagacacatc gctatgtttt tccaacatac caggatgcaa gcccaatgcg
    45361 gcatacagcg gttgataatt ttccgctaac gcctgcacgc gggcaaaatt ttccgcctct
    45421 gtcgccggaa caatgatctt gcctacgccc gcttgtgccg cgcgttgcag gctggcctct
    45481 tcatcgccaa taaaaggcgg gaaatcaaaa tggcagtgag tgtcgataaa acggcaaatc
    45541 aagccagatc ctcattgtta aatgtcgggt cgttcgcctg cggagcgtcg ctcacttcgg
    45601 caaccagcgt atcgttggcg actggcgcag gcggaatgac aatcgattcc ggcgtcacca
    45661 ctggcaccag atggcgggta agcggcgctt tttcagttaa tagcttgcca accgtggcga
    45721 ggaaataacg accgcataaa cgcccaagtt tatagtcttc acgcagcgcc ggaatccgac
    45781 tgccaagcgc gatactatgt aatggcttcg gcggataaat ttcggatatc cgcagcttgc
    45841 ccggtggttt ctcaataaat tgctgaatgt cacgatagct ggtttcatga tgctgcacca
    45901 gattgaccag cggctgcagg ctactgccac ccagccagcg ttccatgcgt ttgaaccact
    45961 gcggcgtgta gtacatttgt gacggcacag tgcggatgac gaccaacgtt ttagcgccct
    46021 gccttgccgc ctctttaacc ggaatcgcat cactgatccc gccatccagg tagttaatgc
    46081 cttccagcga cactccgcta cgataaaagc caggtatcgc actggaggcg cgaatcacat
    46141 ccagccagtt ttgtttggtt ggtaaaaagt aattcggcgc gtagtcatcc tgacgacagg
    46201 cgcacatata aaacgatttg ccgctgtcaa acaaccgcgc ggcggtatcc atttgcaacg
    46261 gcatctggct tgcggtggcc tccaccagcc agtcgagatc gataagattt cctccacgga
    46321 caaagcgcaa tggatcgaaa aattcgcgtt ttgtggtata gcgcatgatg actttgcgcg
    46381 cgtaaccggg ctgattgcag ataaacgccg agaggttctg cgccccggca gatgtgccga
    46441 gataaagatc gaaaggatta aactgcgcgc gcataaactc atccagcacg ccagccgtga
    46501 aaattccacg ctgtccgccg ccttcgcaca ccagagctat tcgtccaggc tggaacggcc
    46561 ttagcgacaa cggcgcaata ttaccaagcg ttacaggtat tcgctgcccc acttctgcct
    46621 tcctgttttt gttgtgaaag aaagacaaag taacgcaatt tattattcgc taaaaccgca
    46681 aaagccagtc cgctggactg gctttggata aaattttctt aatggtctga aatctagggt
    46741 cgttttcggc ccatgaacaa actcactagg aacagaataa tcccgacgac aaagacaatt
    46801 ttagctgcgc ctgcagcggt accggccaga ccaccaaacc caagtgcggc ggcgattaac
    46861 gcgataacca gaaatatgat gccccaacga aacataagcg tctcctttac catagttaat
    46921 gtcaccgctt gatatgagcg tgaaaatcac tcaatgcgat atttttagtg tggtgcacat
    46981 cgcgcctccc gacaaagttc gggaggacga attacgacga attacttagt tttcagatca
    47041 tttttaacgc ttttcacacc atctaccgct ttggcgatac tttcagcacg gtcactttgt
    47101 gcctgagaat ctacggtacc ggagagctga accacgccat cggtggtttc aactttcact
    47161 ttacgtgaag ggacgatatc gtccgccagc agtttggctt tgatttcact ggtggtggcg
    47221 gtgtcacccg cgtagtcctt caccgagcct tctttagcgt cgcgaacgtg cagtttgtcg
    47281 ctgacagagg tcacgccttc aacgcctttc gccactttca ctgcctcttc ggcctgggcc
    47341 tgactttcaa cgaaaccgct cagggtcacg actttttgat cggttttgac agagatatcg
    47401 gtgctcttga tgttgtcatg atccaccagc gccgccttca ctttcgcggt gatggcgctg
    47461 tcatccatga aattaccgac tttattcata gagctatcga ctttttgccc tgcgctttca
    47521 ttggtagtct gcgcgttgtt ttccgcgtag gcagagccgg tcgcgacggc agaggtcaac
    47581 attacagcca gcagagtttt cgaaatcttc agtcttgtca tagtcatcga tttattcctg
    47641 tatgtttgct cgtaatttga gcctagcaac acgaggttgc attgctgaat agggagagac
    47701 ttcaccctct acagaagtca atggtcgcca tcacaaaagc gatgggtgat gaataacgac
    47761 cattacagtc tctgaatcag ttattaatat cggtagaatg acaatcgacg gctttagata
    47821 ctgatatcta cgcattgaac ggtatttaac gccatcagaa atgtcatcac tttgttaaat
    47881 atagatcaca attttgaaac cgctcgggat atcagcgaaa acataagcaa aagtgaatgt
    47941 tttaagaaca ttccgtaagc ggctaataag gaagggaaat tgacagggcg cagcggctac
    48001 cgcgccctgg aggcaagaat taatgctcgc gggtctggtg gaactgaacg tccggataac
    48061 gttcctgtgc caggcgcagg ttgaccatgc tggtagcgat gtaagcgagg ttatcgccgc
    48121 catcaagcgc cagttggctt tcgttcttac gcttgaactc ttcgaatttc ttcgcgtctg
    48181 cacattctac ccagcgggca gtggcaacgt tgactgactc atacactgct tcaacgttgt
    48241 attcgctctt caggcgcgct accaccacat caaactgcag cacaccaacc gcaccaacga
    48301 tcagatcgtt gttggagatt ggacggaaca cctgcaccgc gccctcttcg gaaagctgta
    48361 ccagcccttt gagcagctgt ttttgcttca gcggatcttt caggcggata cgacggaaca
    48421 gttctggtgc gaagttcgga ataccggtga acttcatcat ctcaccctgg gtaaaggtgt
    48481 cgccgatctg aatggtgccg tggttgtgca ggccgaggat atcgcccgga tacgcttctt
    48541 caacgtgcga acggtcaccc gccataaagg tcagcgcgtc ggagatcacc acatctttcg
    48601 cagtgcgcac ctggcgcagt ttcatgcctt tttcatattt accggacacc acacgcataa
    48661 acgccacgcg gtcgcggtgt ttcgggtcca tgttggcctg aattttaaat acgaagccgg
    48721 taaatttatc ttcgctcgct tctacggtac gggtatcggt ctgacgcggc atcggcgcag
    48781 gcgcccactc caccaggcca ttcagcatat gatcaacgcc gaagttaccc agcgcagtac
    48841 cgaagaatac cggagtgatt tcgcccgcaa ggaacagctc tttgtcgaac tcgttagacg
    48901 cgcctttaac cagttccagt tcgtcacgca gctgctgtgc cagatcttca ccaaccgcag
    48961 catcgagatc cgggttattc agccctttaa caatgcggac ttcctgaatg gtgtgccctt
    49021 taccgctctg atagagatag gtttcatctt tataaaggtg gtaaacgcct ttaaacagct
    49081 tgccgcagcc aattggccag gtgatcggtg cgcagccaat tttcagctcg ttctcaactt
    49141 catcgagaag ttccatcggg tcgcggatat cacggtcaag tttgttcata aaggtgagga
    49201 tcggcgtgtc gcgcagacgg gtaacttcca tcagcttacg ggtacgatct tcaacacctt
    49261 ttgcggcgtc gataaccatc aggcagcagt ccaccgccgt cagggtacga taggtatctt
    49321 ccgagaagtc ttcgtgcccc ggggtgtcga gcaggttaac caggcaatcg tgatacggaa
    49381 actgcatcac agacgtagta atggagatcc cacgctgctt ttccatctcc atccagtccg
    49441 acttagcgtg ctggttggaa ccacggcctt ttactgtacc ggcggtctga atggcctgtc
    49501 cgaacagcag caccttctcg gtgatggtag ttttaccggc gtccgggtga gaaataatgg
    49561 caaaagtgcg gcgcttcgcc acctcttgca aataaggaga caacgtcata attctttctt
    49621 cttgagtaag cggcatcgcg ccgcgcttgt tgaaaacgaa aaattgcggc tattttaccc
    49681 atcaacgggg gggaggcaat cagtgtttac acaggagctg ctccagttcg tgcaacgaag
    49741 aaacggtcca ggtgggcgcg atgccttctg gttgctcgcg attgtgcgca ttcagccagc
    49801 aggtcgcaag cccggcgttg atgccaccga gaatatcgga ctcggcagtg tcgccaacca
    49861 tcagcacgcg tgaacggtca ggattgcccg cctgttccag cgcataatcg aaaattttct
    49921 tattcggttt ggcaacgcca acttcttcgg aaatcaccag caaatcgaag taatcacgca
    49981 ggcccgtccg ttccagacgc acttgctgca aggcgctaaa accgttggtg atgatgccga
    50041 ttttagcgtt gccacgaatg gcgttaagca gagaaaccgc gcccggcaac ggcgtacaga
    50101 tttccgccat cgcattaata aaggcatcgt taagcaagcc tgcttcaact ttcagccgtt
    50161 cggcccagct ctcgaaacgc ccgtgctgaa gctgtaatga agtgatcgcg ccgttttgat
    50221 aatccaccca cagtggcttg ttaacggcct gatagtcctg aaaatcttca gcggtaaagg
    50281 tgacgctgta atcaagaaac atccgctgca ggccggtgaa tgagtcaaag gtaaacagcg
    50341 tttcatcggc atcaaagaaa atccagtccc acttcattat tccaccttgt cttacatact
    50401 gattggcaac gccatgatga tggcgtcttc gcgaccgtcc gtggtggggt agtaattgcg
    50461 gcgaatcgtc gcctcgttaa agcctaaact ttcgtacagg gcaatggcgg cagcatttga
    50521 ggcacggact tccagccata gtgtcgccac gccgcgtttt tccagttcgt cgatcagatg
    50581 ttccagcagc gccctaccca gcccctgacg ctgatagtcg ggatcgaccg caatattgaa
    50641 caatgtagct tcatccaaca ccacttgcgt aatcgcaaac gccgccattt tgccgttttg
    50701 cgttaactga aagttgagat aacgctcgcc ctggttgctg gcaaaggttt tttcactcca
    50761 cggaaaggcg tgggcgcgtt gttcaatgtg gtaagccgct ggtaaatcag tcgtttcgag
    50821 ggaagaaatc gtgttcatat gtgcaaattt gttgccataa cgcggcgcgt gccgttgggt
    50881 ttgcccgtaa ttcggtgagc gccggtgatg ccacctgagc gccttccaat gatagcggtt
    50941 cgtcagtacc caaccgccag ctgttgcagc gactgccttg cggcagcatc gcgatttttt
    51001 ctggcgtcag ttgcagcact tgatcggggc tgacggttaa tgcgcgcaga acatcgctca
    51061 ctaaaggatc agtcagggcg ggaagatcgt ttgccaccat caccagacgg acgtgtgccg
    51121 ggctcgcaat ggcaatctcc ccctgcaacg cgccagggcg acgcagcgac cactgggtaa
    51181 tgcccagttg ctgtaactgc cagtctcgtc gggatgtcat agcgaatcgc tcctgttgtc
    51241 aggggcgcaa atatagcaaa ttcgtcgata ccgcgccaac atatggctat aatcgccgcc
    51301 agtatcaatt gaggagcatt ccatgtctgc atttaccccg gcaagtgaag tcttgctgcg
    51361 tcacagtgat gatttcgaac aaagccgtat tctgtttgcc ggagacttac aggatgacct
    51421 gcccgcgcgt ttagataccg cggccagccg tgctcatacc cagcaattcc accattggca
    51481 ggtgttaagc tgccagatgg gggataacgc ccgttttagt ctggtcgcca cggcgaatga
    51541 tgtcgcagat tgcgatacgc tgatttacta ctggccgaag aacaaaccgg aagcccagtt
    51601 ccagttgatg aatttacttt ctctgctgcc agtggggaca gatatttttg tcgttggcga
    51661 gaaccgcagc ggcgtgcgca gcgctgagca gatgctggca gattatgcgc cgttgaataa
    51721 agtcgacagc gctcgtcgct gtggcctcta ttttggtcgt ctggaaaaac agccagtatt
    51781 tgatgcggat aaattctggg gcgaatacag cgtcgatggc ctgacggtca aaacgctgcc
    51841 tggcgtgttt agccgcgacg gtctggatgt cggtagccag ttgctgctct cgacgttaac
    51901 tccgcacacg aaaggtaaag tgctggatgt cggctgtggc gcgggtgtac tttcagttgc
    51961 ctttgcgcgc cattcgccga aaattcgtct caccttgtgc gatgtctctg ccccggcggt
    52021 agaagccagc cgcgcaacac ttgcagccaa cggtgttgaa ggtgaagtct ttgccagcaa
    52081 cgtcttttcc gaggtgaaag gttgttttga tatgatcatc tccaacccgc cgttccacga
    52141 tgggatgcaa accagcctgg atgcggcgca aacgctgatt cgtggcgcgg tgcgtcatct
    52201 taatagcggc ggcgagctgc gaattgtagc gaacgccttc ctgccttacc cggacgtgct
    52261 ggatgagaca tttggcttcc atgaagttat cgcgcaaacc gggcgcttca aggtatatcg
    52321 caccattatg acccgccagg cgaagaaagg ttgagtatct gatatctgtc gccgcatacc
    52381 gtgtcggatg cggcgtgaac gccttatccg acctacaaaa cctgcacgct agtttcctga
    52441 tggacatttt tccagcaatt acacatcagg cgataattaa ctattgacga aaagctgaaa
    52501 accactagaa tgcgcctccg tggtagcaat tctttttaag aattgatgg
//
LOCUS       DADMEQ010000017        47489 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.017, whole genome shotgun sequence.
ACCESSION   DADMEQ010000017 DADMEQ010000000
VERSION     DADMEQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.017"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(2..94)
                     /locus_tag="JNZ05_001018"
     tRNA            complement(2..94)
                     /locus_tag="JNZ05_001018"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="JNZ05_001019"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="JNZ05_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HAY8564335.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="JNZ05_001020"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="JNZ05_001020"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAY8564336.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="JNZ05_001021"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="JNZ05_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAY8564337.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="JNZ05_001022"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="JNZ05_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAY8564338.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="JNZ05_001023"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="JNZ05_001023"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HAY8564339.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYVLQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="JNZ05_001024"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="JNZ05_001024"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HAY8564340.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8018
                     /gene="srlA"
                     /locus_tag="JNZ05_001025"
                     /gene_synonym="gutA"
     CDS             7455..8018
                     /gene="srlA"
                     /locus_tag="JNZ05_001025"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAY8564341.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8015..8974
                     /gene="srlE"
                     /locus_tag="JNZ05_001026"
                     /gene_synonym="gutE"
     CDS             8015..8974
                     /gene="srlE"
                     /locus_tag="JNZ05_001026"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAY8564342.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8985..9356
                     /gene="srlB"
                     /locus_tag="JNZ05_001027"
                     /gene_synonym="gutB"
     CDS             8985..9356
                     /gene="srlB"
                     /locus_tag="JNZ05_001027"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAY8564343.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSMAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9360..10139
                     /gene="srlD"
                     /locus_tag="JNZ05_001028"
     CDS             9360..10139
                     /gene="srlD"
                     /locus_tag="JNZ05_001028"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HAY8564344.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10244..10603
                     /gene="gutM"
                     /locus_tag="JNZ05_001029"
     CDS             10244..10603
                     /gene="gutM"
                     /locus_tag="JNZ05_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HAY8564345.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGHFKPRVVVAIALDDQQCIVDILFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10670..11443
                     /gene="srlR"
                     /locus_tag="JNZ05_001030"
                     /gene_synonym="gutR"
     CDS             10670..11443
                     /gene="srlR"
                     /locus_tag="JNZ05_001030"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HAY8564346.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11436..12401
                     /gene="gutQ"
                     /locus_tag="JNZ05_001031"
     CDS             11436..12401
                     /gene="gutQ"
                     /locus_tag="JNZ05_001031"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HAY8564347.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12398..13912)
                     /gene="norR"
                     /locus_tag="JNZ05_001032"
     CDS             complement(12398..13912)
                     /gene="norR"
                     /locus_tag="JNZ05_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HAY8564348.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRVVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14099..15538
                     /gene="norV"
                     /locus_tag="JNZ05_001033"
     CDS             14099..15538
                     /gene="norV"
                     /locus_tag="JNZ05_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HAY8564349.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHCREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15535..16668
                     /gene="norW"
                     /locus_tag="JNZ05_001034"
     CDS             15535..16668
                     /gene="norW"
                     /locus_tag="JNZ05_001034"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HAY8564350.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16796..19048)
                     /gene="hypF"
                     /locus_tag="JNZ05_001035"
     CDS             complement(16796..19048)
                     /gene="hypF"
                     /locus_tag="JNZ05_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAY8564351.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19201..19728)
                     /gene="hydN"
                     /locus_tag="JNZ05_001036"
     CDS             complement(19201..19728)
                     /gene="hydN"
                     /locus_tag="JNZ05_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HAY8564352.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19877..20890)
                     /locus_tag="JNZ05_001037"
     CDS             complement(19877..20890)
                     /locus_tag="JNZ05_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY8564353.1"
                     /translation="MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEES
                     SYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKH
                     SAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSV
                     WCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLI
                     ANGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGF
                     DDIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIA
                     LSR"
     gene            21147..22604
                     /gene="ascF"
                     /locus_tag="JNZ05_001038"
     CDS             21147..22604
                     /gene="ascF"
                     /locus_tag="JNZ05_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HAY8564354.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVPWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQSVQPTVAKEVSLN"
     gene            22613..24037
                     /locus_tag="JNZ05_001039"
     CDS             22613..24037
                     /locus_tag="JNZ05_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY8564355.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQDD
                     EITPNQQGIAFYRPVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYTSRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24152..24571)
                     /locus_tag="JNZ05_001040"
     CDS             complement(24152..24571)
                     /locus_tag="JNZ05_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY8564356.1"
                     /translation="MTANAAHAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(24568..24879)
                     /locus_tag="JNZ05_001041"
     CDS             complement(24568..24879)
                     /locus_tag="JNZ05_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HAY8564357.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLGVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25052..25795)
                     /locus_tag="JNZ05_001042"
     CDS             complement(25052..25795)
                     /locus_tag="JNZ05_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564358.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFDKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            complement(25847..26317)
                     /gene="hycI"
                     /locus_tag="JNZ05_001043"
     CDS             complement(25847..26317)
                     /gene="hycI"
                     /locus_tag="JNZ05_001043"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HAY8564359.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(26310..26720)
                     /locus_tag="JNZ05_001044"
     CDS             complement(26310..26720)
                     /locus_tag="JNZ05_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HycH family protein"
                     /protein_id="HAY8564360.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARHKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(26717..27484)
                     /gene="hycG"
                     /locus_tag="JNZ05_001045"
     CDS             complement(26717..27484)
                     /gene="hycG"
                     /locus_tag="JNZ05_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HAY8564361.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGILHDLYCVWGGTDKIVPVDVYIPSCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(27484..28026)
                     /gene="hycF"
                     /locus_tag="JNZ05_001046"
     CDS             complement(27484..28026)
                     /gene="hycF"
                     /locus_tag="JNZ05_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HAY8564362.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28036..29721)
                     /locus_tag="JNZ05_001047"
     CDS             complement(28036..29721)
                     /locus_tag="JNZ05_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HAY8564363.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDDWPDELYPLRKDSMDYRQRPAPTTDAETY
                     EFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAET
                     RMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSH
                     LLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRHDLLKDDM
                     IQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIACDFSNVGPMVRASGLA
                     RDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLM
                     VEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGN
                     TVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(29739..30635)
                     /gene="hycD"
                     /locus_tag="JNZ05_001048"
     CDS             complement(29739..30635)
                     /gene="hycD"
                     /locus_tag="JNZ05_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HAY8564364.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIGSPLPQLGDLITLLYLFAIARFFF
                     AISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPL
                     SQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLK
                     QLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDI
                     TPRITWAGFGFAFLAFVSLLAA"
     gene            complement(30638..32527)
                     /gene="hycC"
                     /locus_tag="JNZ05_001049"
     CDS             complement(30638..32527)
                     /gene="hycC"
                     /locus_tag="JNZ05_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HAY8564365.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGSVGVSGAMSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGSDIWLLGVIGFGLLAGI
                     IPLHGWVPQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMI
                     TAFVGGLYALMEHNIQRLLAYHTLENIGIILLGLGLGLGLGLGLGLGLGAGVTGIALE
                     QPALIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIA
                     MLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVM
                     CMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAV
                     PLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSM
                     VITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVII
                     VSRGA"
     gene            complement(32524..33135)
                     /locus_tag="JNZ05_001050"
     CDS             complement(32524..33135)
                     /locus_tag="JNZ05_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY8564366.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPLLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVSGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33260..33721)
                     /gene="hycA"
                     /locus_tag="JNZ05_001051"
     CDS             complement(33260..33721)
                     /gene="hycA"
                     /locus_tag="JNZ05_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HAY8564367.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            33933..34283
                     /gene="hypA"
                     /locus_tag="JNZ05_001052"
     CDS             33933..34283
                     /gene="hypA"
                     /locus_tag="JNZ05_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY8564368.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            34287..35159
                     /gene="hypB"
                     /locus_tag="JNZ05_001053"
     CDS             34287..35159
                     /gene="hypB"
                     /locus_tag="JNZ05_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HAY8564369.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            35150..35422
                     /gene="hypC"
                     /locus_tag="JNZ05_001054"
     CDS             35150..35422
                     /gene="hypC"
                     /locus_tag="JNZ05_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HAY8564370.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            35422..36543
                     /gene="hypD"
                     /locus_tag="JNZ05_001055"
     CDS             35422..36543
                     /gene="hypD"
                     /locus_tag="JNZ05_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HAY8564371.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            36540..37550
                     /gene="hypE"
                     /locus_tag="JNZ05_001056"
     CDS             36540..37550
                     /gene="hypE"
                     /locus_tag="JNZ05_001056"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HAY8564372.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDCLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAAVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            37624..39702
                     /gene="flhA"
                     /locus_tag="JNZ05_001057"
     CDS             37624..39702
                     /gene="flhA"
                     /locus_tag="JNZ05_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HAY8564373.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(39739..40092)
                     /locus_tag="JNZ05_001058"
     CDS             complement(39739..40092)
                     /locus_tag="JNZ05_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HAY8564374.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(40124..40303)
                     /locus_tag="JNZ05_001059"
     CDS             complement(40124..40303)
                     /locus_tag="JNZ05_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564375.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMFMKQVRKIVSIG
                     EPFFFRVIVPFKIKM"
     gene            40379..42940
                     /gene="mutS"
                     /locus_tag="JNZ05_001060"
     CDS             40379..42940
                     /gene="mutS"
                     /locus_tag="JNZ05_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAY8564376.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            43046..43698
                     /gene="pphB"
                     /locus_tag="JNZ05_001061"
                     /pseudo
     CDS             43046..43698
                     /gene="pphB"
                     /locus_tag="JNZ05_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(43749..44546)
                     /locus_tag="JNZ05_001062"
     CDS             complement(43749..44546)
                     /locus_tag="JNZ05_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY8564377.1"
                     /translation="MSAGQRKEQKLIPVERRQIILEMVAEKGIVSIAELTDRINVSHM
                     TIRRDLQKLEQQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQP
                     GSCIYLDAGTTTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENR
                     SCVGEAAATMLRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDA
                     TKYGQVATWLALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            44712..45620
                     /locus_tag="JNZ05_001063"
     CDS             44712..45620
                     /locus_tag="JNZ05_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY8564378.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            45617..46879
                     /locus_tag="JNZ05_001064"
                     /pseudo
     CDS             45617..46879
                     /locus_tag="JNZ05_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
     gene            46876..>47489
                     /locus_tag="JNZ05_001065"
     CDS             46876..>47489
                     /locus_tag="JNZ05_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY8564379.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAG"
BASE COUNT        11234 a        12965 c        12366 g        10924 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatcg tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 aacgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaacga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata acctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgacccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctacgcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tacatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaagttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac gccccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttgcgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggaa tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgctggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctaggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tggcgaactg tttgtttatc ttggcattgc cagcggtctg acaacgctga
     7861 acctgccact tggcccactg gcggtgagtt atctgctggt tggtctggtc accaatttct
     7921 tccgcggctg ggtgaccgat ctgaccaccg ccatttttga gaaaaagatg ggcattcaac
     7981 ttgaacaaaa agttcacctg gcaggagcaa catcatgacg cgtattcgga tcgaaaaagg
     8041 aacgggtggc tggggcggcc cgcttgagct ggaagctacg ccgggcaaaa aaatcgtcta
     8101 tatcaccgcc ggtacccggc ctgcgattgt tgacaaactg gcacaactta ctggctggca
     8161 ggctattgac ggatttaaag aaggtgaacc cgcggaggcg gaaattggtg tcgcggtaat
     8221 cgactgtggc ggcacattac gctgcggcat ctatccgaaa cgacgtattc ccaccattaa
     8281 tatccactcg acgggcaagt ccggtccgct ggcgcagtac attgtggaag atatttatgt
     8341 ctctggcgta aaagaagaaa acatcactgt agtgggtgat gcgacaccac aaccctcttc
     8401 cgtgggccgt gactatgaca ccagtaagaa aatcaccgaa caaagcgatg gtttactggc
     8461 gaaggtggga atgggcatgg ggtccgccgt tgccgtgttg tttcagtctg gtcgtgacac
     8521 catcgacact gtattaaaaa ccattctgcc gtttatggca ttcgtctcgg cgctcattgg
     8581 catcattatg gcttctggcc ttggtgactg gattgcccac ggtcttgctc cgctggcgag
     8641 ccatccactg ggtctggtca tgctggcgct catctgctcc ttcccactgc tttcaccttt
     8701 cctcggccca ggcgcagtta tcgcacaggt tatcggcgta ttgattggcg tgcagattgg
     8761 tctcggcaat attccgccgc atctggcttt accggcactg tttgccatca acgcgcaggc
     8821 ggcctgcgac ttcatcccgg tcggtttgtc gctggcggaa gcccgtcagg acacggttcg
     8881 cgtcggtgtc ccttctgtac tggtgagccg ctttttaacc ggcgcgccga ctgtactgat
     8941 cgcctggttt gtctccggtt ttatctatca atagaggctg aaacatgacc gttatttatc
     9001 agaccaccat cacccgtatc ggcgcgagtg ccactgacgc cctcagcgac cagatgctca
     9061 tcacctttcg tgaaggcgcg ccagcggacc tcgaagagta ttgcttcatt cattgccacg
     9121 gcgagttgaa aggtgcactc catcccggtt tgcaattttc actcgggcag catcgctatc
     9181 cggtgaccgc tgttggcagc atggcggaag acaaccttcg cgaactgggt catgtcaccc
     9241 tgcgcttcga tggtttaaac gaagcggaat ttccgggcac tgtccatgtg gcaggccctg
     9301 tacccgacga tatcgcgccg ggatcggttt tgaagtttga atctgttaag gagtaaaaaa
     9361 tgaatcaggt tgccgttgtc atcggtggtg ggcaaacctt aggcgcgttc ctgtgccacg
     9421 gtctggctgc cgaggggtat cgcgtcgcgg ttgtcgatat tcagagcgac aaagccgcaa
     9481 atgtggcaca agaaattaac gccgaatatg gtgaaggtat ggcgtacggt tttggtgctg
     9541 acgccactag cgagcaaagc gttctggcgc tctctcgtgg ggtagatgaa atctttggtc
     9601 gcgtggattt gctggtctat agcgccggaa tagcaaaagc agcctttatc agcgacttcc
     9661 agctcggcga ttttgaccgt tcgctacaag tgaatctggt gggttatttc ctgtgtgccc
     9721 gtgaattttc gcgtttgatg atccgcgacg ggattcaggg tcgcattatt cagatcaact
     9781 cgaaatccgg caaagtgggc agcaaacaca actctggcta cagcgcagcg aaatttggtg
     9841 gcgtcgggct gactcaatca ctggcgctgg atctggcgga gtacggcatt acggtgcatt
     9901 cactgatgct cggtaacctg ctgaaatcgc cgatgttcca gtcactgttg ccacaatacg
     9961 cgaccaagct gggtatcaaa ccggatcaag tcgagcagta ttacatcgac aaagtaccgc
    10021 tcaaacgcgg ctgcgattat caagatgtgc tgaatatgct gctgttctac gccagtccta
    10081 aggcgtcgta ctgcaccgga cagtcgatca atgtcaccgg cggtcaggtg atgttctgat
    10141 caacagcgga gatccgttaa ggatctccgt gagactatag aatgcctgat gcgctacgct
    10201 catcaggcat acaggacttc cgccactaca ttaaggaaaa gttatggtat ccgcactcat
    10261 caccgtcgcc gttatcgcct ggtgtgcgca actggcctta ggcggctggc aaatttctcg
    10321 ttttaaccgt gccttcgaca cactatgcca gcaagggagg gttggcgtgg gccgttccag
    10381 cgggcacttt aaaccgcggg tcgtggtcgc catcgcgctg gacgatcagc agtgcatcgt
    10441 cgacatcttg tttatgaaag gactgactgt cttcgcccga ccgcaaaaaa ttcccgcaat
    10501 taccggcatg catgcgggtg atttacagcc cgatgtgatc tttccccatg atccactatc
    10561 acagaatgct ctatcattgg cgcttaaact gaaacgtgga taatttcgtt gtgaatgtta
    10621 cttgcttgcg aagttatcat tttgaaacct aaatcaggta atcacgccca tgaaacctcg
    10681 tcagcgtcag gccgccattc tggagtatct gcaaaagcag ggtaaatgct cggttgaaga
    10741 attggcgcaa tactttgaca ccacaggcac aaccattcgc aaagatctgg tcattctgga
    10801 acatgccgga accgtcattc gtacttatgg cggagtggtg ttgaataaag aggaatccga
    10861 tccgcctatc gatcataaaa cgctcatcaa cacccacaag aaagagctga ttgcagaagc
    10921 tgccgttagt tttatccatg atggcgattc gatcattctt gatgctggca gtaccgtttt
    10981 gcagatggtt cccctgctct cgcgctttaa taacatcacg gtgatgacca acagcctgca
    11041 tatcgtcaat gcgctatccg aactggataa cgaacaaact atcctgatgc caggcggaac
    11101 atttcgcaaa aaatcggcct catttcacgg gcaactggca gagaatgcct tcgagcattt
    11161 caccttcgat aaattgttta tgggcaccga cggcatcgat ctcaatgcgg gcgtaaccac
    11221 gttcaacgag gtttataccg tcagtaaggc aatgtgcaat gccgcgcgcg aagtgatttt
    11281 gatggcggac tcatcaaagt ttggccgtaa aagccccaac gtagtttgca gtcttgaaag
    11341 cgtcgataag ctaattaccg acgcaggtat cgatccggcg tttcgtcagg cgctggaaga
    11401 gaaagggatc gatgtgatca taaccggaga gagcaatgag tgaagcacta ctgaacgcgg
    11461 gacgtcagac gttaatgctg gagttgcagg aagcaagccg tttaccggaa cgtctgggcg
    11521 atgattttgt tcgcgccgcc aatatcatcc tgcactgcga aggcaaagtg gtggtttcgg
    11581 gaattggcaa atcgggccac attggtaaga aaatcgccgc aacacttgcc agtaccggca
    11641 caccggcttt ttttgtccat ccggcagaag cactgcacgg cgatctgggg atgattgaaa
    11701 gccgcgatgt gatgctgttt atctcttact ccggtggcgc gaaggaactg gatctgatta
    11761 ttccgcgtct ggaagataaa tctatcgcgc tgctggcgat gaccggcaaa ccgacgtcac
    11821 cgctgggcct ggcggcgaaa gcggtgctgg atatctccgt agaacgcgaa gcctgcccga
    11881 tgcaccttgc gccgacctcc agcaccgtca ataccctgat gatgggtgac gcgctggcga
    11941 tggcggtcat gcaggcgcgc ggttttaatg aagaagattt tgcccgctcc cacccagctg
    12001 gggcactggg cgctcgcttg ctgaataaag tgcatcatct gatgcgtcgt gatgacgcta
    12061 tcccacaggt agcgttaacc gccagcgtga tggatgcgat gctggaactc agccgcaccg
    12121 gtctggggct ggtggcggta tgtgacgctc aacaacaggt acaaggcgtc tttaccgacg
    12181 gcgatttacg tcgctggctg gtaggcggcg gcgcactcac cacgccagtt aatgaagcga
    12241 tgacgacagg cggcaccaca ttacaggcac aaagtcgcgc catcgacgcc aaagaagttc
    12301 tgatgaaacg caaaatcact gccgcaccag tggtagatga aaacggcaaa ctcaccggcg
    12361 caattaacct gcaggatttc tatcaggccg ggattattta atccttcaat cccagacgtt
    12421 tcgccagccg atgcaggttg gcgacgtcag tttccagcat tcgcgcacag gctgcccagt
    12481 tgtgatgatt ttgtgccagt gcctggcgaa tagtttcacg ctggaacgct tctgtcgctt
    12541 cacgcaggtt ttgcttaaca acgggcaccg ccgccgcttc tggcggcggc aacgtcacct
    12601 caggaaaagc aaaatgttgc gcctcaagaa tcacttcatc gccgttgcgg gtggctctcg
    12661 ccagcactac cacccgatga atagcatgtt ccagttcgcg cacgtttccc ggaaaactgt
    12721 agtgttgcag taaatttcgc gccccggcac ttaataccac gcgggagagc ccctgccgca
    12781 aacgacactg ctcgcagaaa taccccgcca gcagaatgac atcatcgccc cgctcacgca
    12841 gcggcggcac cgaaagtgga aacacgctca ggcgatgaaa caaatcggcg cggaatcgtc
    12901 ctgccagcac ctcttcgcgt aagtcgcggt tagtcgccgc cagcacgcgc acatcgaccc
    12961 gcaaactacg gtcatcgcca acgcgctgaa tatcgccata ctgcaacacc ctcagcagct
    13021 tggcctgcaa tgccaacgac aactcgccga tctcatcgag aaacagagta ccgttatccg
    13081 ccatttcaaa cttcccgctg cggttactga tagcgccagt aaacgctcct ttcacatgcc
    13141 cgaacaactc actttccgcc acactttccg gcagtgcggc acagttgaga tagaccagcg
    13201 gattcaccgc ccgtggcgag gcttcatgaa tcgctttcgc caccagctcc ttaccggttc
    13261 ccgtctcacc gctgatcaga acgttgagat cggacgccgc cacaatctca atctcttttt
    13321 tcagttgcgt catgccaggg gacaagccaa tcatctgcgt ctgtttcacc gcttcaaacg
    13381 gcgtggcatc gcctggcagc atattctggc tttccagttg ttcaatcagc aacgcattgc
    13441 ttaacgctcc cgccgccagc gcggcaatca gccgtaactc ttcgtcgctg aaaacatcga
    13501 actgatcggg ctgcatcccg tcgagcgtca gtgcgccgat caggttttgt ccgacaaaca
    13561 atggcagacc aacgcaggcg tgaaccttca gactctcctg cccaggaatc aaaccgtcat
    13621 agggatcggg caattcgctg tcagcgggaa agcgcaccac atccccggcg cgggcaatcg
    13681 cttccagccg tggatgccct tccagcgcaa agcgtctacc gagtacatcc tttgccagac
    13741 cgtcgatggc aagcggaata aactgccgcg aatcgtaacg tagcaacgca gacgcatcgc
    13801 actccagcac ctgacgtagc gtggtgatca ggcgctgaaa acgatcctga tgaccaatcc
    13861 cacgctgcaa ttcgatggcg atattcgcca gcacatcaac ggaaaaactc atctttgcct
    13921 cactgtcaat ttgactatag atattgtcat atcgaccatt tgattgatag tcattttgac
    13981 tactcattaa tgagcataat tttatttata gagtaaaaac aatcagataa gaaactggca
    14041 cgcaatctgc aattagcaag acatcttttt agaacacgct gaataaattg aggttgctat
    14101 gtctattgtg gtgaaaaata acattcattg ggtgggtcaa cgtgactggg aagtgcgtga
    14161 ttttcacggc acggaatata aaacgctgcg cggcagcagc tacaacagct acctcatccg
    14221 cgaagaaaaa aacgtgctga tcgacaccgt cgaccataaa ttcagccgcg aatttgtgca
    14281 gaacctgcgt aatgaaatcg atctggcgga tatcgattac atcgtgatta accatgcaga
    14341 agaggaccac gccggggcgc tgaccgaact gatggcgcaa attcccgata cgccgatcta
    14401 ctgtactgcc aacgctatcg actcgataaa tggtcatcac catcatccgg agtggaattt
    14461 taatgtggtg aaaactggcg acacgctgga tatcggcaac ggcaaacagc tcatttttgt
    14521 cgaaacacca atgctgcact ggccggacag catgatgact tacctgacag gcgacgcggt
    14581 gctgttcagt aacgatgctt tcggtcagca ctactgcgac gagcatctgt tcaacgatga
    14641 agtggatcag acggagcttt tcgagcagtg ccagcgttac tacgccaata tcctgacgcc
    14701 gttcagccgc ctggtaacgc cgaaaattac cgagatcctg ggctttaact taccagtcga
    14761 tatgatagcg acctcccacg gcgtggtctg gcgcgataac ccgacgcaaa ttgtcgagct
    14821 gtacctgaag tgggccgctg attatcagga agacagaatc accattttct acgacaccat
    14881 gtcgaataac acccgcatga tggctgacgc tatcgcccag ggaattgcgg aaaccgaccc
    14941 acgcgtggcg gtgaaaattt tcaacgtcgc ccgaagcgat aaaaacgaaa tcctgaccaa
    15001 tgtcttccgc tcaaaaggcg tgctggtcgg cacttcgacg atgaataacg tgatgatgcc
    15061 gaaaatcgcc gggctggtgg aggagatgac cggattacgc ttccgtaaca aacgcgccag
    15121 tgctttcgga tctcacggct ggagcggcgg tgcggtggat cgcctttcca cgcgcctgca
    15181 ggatgcgggt ttcgaaatgt cgctcagcct gaaagccaaa tggcgaccag accaggacgc
    15241 tctggagtta tgccgtgaac actgtcgcga aatcgcccgt cagtgggcgc tcgcgccgct
    15301 gccgcagagc acggtgaata cggtagttaa agaagaaacc tctgccacga cgacggctga
    15361 cctcggccca cgaatgcagt gcagcgtctg ccagtggatt tacgatccgg caaaaggcga
    15421 gccaatgcag gacgttgcgc caggaacgcc gtggagtgaa gtcccggata acttcctctg
    15481 cccggaatgc tccctcggca aagacgtctt tgacgaactg gcatcggagg caaaatgagc
    15541 aacggcattg tgatcatcgg ttcgggcttc gccgcccgcc aactggtgaa aaatattcgc
    15601 aaacaggacg ccactattcc attaaccctg attgccgccg acagcataga tgagtacaac
    15661 aaacccgacc tcagccatgt tatcagtcag gggcaacgtg ccgacgacct tacccgccag
    15721 acggcgggtg aatttgccgg gcagtttaat ctgcgcctgt ttccgcacac ctgggtgacg
    15781 gatatcgatg ccgaagccca tgtggtgaaa agccagaata atcagtggca atacgacaag
    15841 ttagtgctgg caaccggtgc cagcgccttt gtcccgcctg tgcctgggcg tgagttaatg
    15901 ctgacgttaa atagtcagca agagtatcgc gcctgtgaaa cgcaactgcg ggatgcccga
    15961 cgcgtgttga ttgtcggcgg tggtttgatt ggtagcgaac tggcgatgga tttttgtcgt
    16021 gcaggcaaag cggtcacgct aatcgacaac gctgccagta ttctggcgtc attaatgcca
    16081 ccagaagtaa gcagccgctt gcagcatcgg ttgacggaga tgggcgttca tctgctgttg
    16141 aaatctcagt tgcaggggct ggaaaaaacg gattctggca ttctggcaac gctggaccac
    16201 cagcgtagta tcgaagtgga tgcggtaatt gccgccaccg gactgcgccc ggaaacagcc
    16261 ctggcacgac gcgccgggct gacgattaat cgcggcgttt gcgtcgatag ttatctgcaa
    16321 accagtaatg ccgatattta cgcgctgggc gattgcgcgg aaattaacgg tcaggtattg
    16381 ccgttcctgc agccgattca acttagcggg atggtgctgg caaaaaatct tctcggcaat
    16441 aacacgccgc tgaaactacc ggcgatgctg gtgaaaatca aaacgccgga attaccgctg
    16501 catctggcag gcgaaaccca gcgtcaggat ttacgctggc agattaatac cgaacgccag
    16561 ggaatggtgg cgcgcggcgt tgacgatgct gaccagcttc gcgcctttgt ggtcagtgag
    16621 gatcggatga aagaggcatt tgtattgttg aaaacgttgc cggtgtaggt gggctactgt
    16681 gcctaaaatg tcggatgcga cgctggcgcg tcttatccga cctacgggga cgcatgtgta
    16741 ggccggataa ggcatttacg ccgcatccgg caagggtgtc caaatgcaac acgctttatc
    16801 cgttctggac ttcacccgct aaccaacgcg ccgcagcaat aaccccctgc cccagagaca
    16861 aaccgccatc acccgccggt aaactctgtg gaaagagcaa tgtgaaatca gcgagataat
    16921 gcgccagacg tgcacgcagc aaacggttat gaataacccc gccgctaaat accagcgtag
    16981 tgataccacg catcgtggcc tgctcacgca tcaacgcggc aaaaccctgc gccagcgcat
    17041 catgaaacgc ccacgcgcgt tgattaaccg gtgcctgcca gttcagccac tgctgccaga
    17101 aagtggcgag atccagttga ttgtccaccc gcggcatcgt caccggatgc gtcactccgt
    17161 cgcatgaggc tgcgagcgcc tccagagcac aagccgcttc accttcataa cttaacgtgg
    17221 ctggcgcaca gcccagtgcc gccgccactg catcgaacaa ccgcccacac gatgatgcca
    17281 gcggcgcgtt aattccacgc tcaatggccc gcgccagtac gctccagttt tgctgttgca
    17341 cacttgccgt ttcagggtaa ttctgccact ccggcacaaa gcgcaggcac tgcgccagca
    17401 ggtttcgcca cggctgcttc gccgccaaat cgccacccgg aagcgccact gcaggcaagc
    17461 cgcccaggtg ctcacattcg cgatagttca cccgcaggca ctcgccgccc cacaaagcgc
    17521 cattctcccc cataccgata ccgtcgagcg tcaaagcaat gacatcaccg ccatccagcg
    17581 gccactgatg ctctgccaga cacgccgctg catgggcatg atgatgcagt accgtttgcg
    17641 tcggcagatt catttcgctc gcccactggc tggagacata gcccggatgc acatcatgca
    17701 caatgtattg cggagtgaaa tcgtagatgt tttgcatcag gcgtaacgct tcgcgccact
    17761 gcatctggat gccatcgtca cttaaatcgc ccagatgctg actcaacacc gcttgttcac
    17821 cgcgcaccag gcagaaggta tttttcagat ccgcgccaag acacagcaca ggcggaacat
    17881 ttttaaagcc cggaggcaaa accagcgcat ccggcacata cccccgcgaa cggcgcagca
    17941 tttcgccgct ttcgcgcacc actgaatcat ccatccgctg cacgatgtcg cggttatgta
    18001 gcaagaatcc gtcggcaatg ccctgcaaat ccgccagcgc ctgttcgttg ctgatagctg
    18061 gtggtttacc gctcaggttg ccggaggtca tcaccagcgg gcattgcagt tcctgtaaca
    18121 gcaaatgctg gagcgggttc gcaggcaaca tcaccccgac ttcgttaagg ccaggggcga
    18181 tatcatcaca aagctcaggt acgtattttt tatccaccag cacaatcggc gcagcgggcg
    18241 tggtaagcaa ctggcgcgca gcgtctggta aactttctgc cactggcaac atgactgcca
    18301 gcggtttcgc cgggcgatgt ttgcgctccc gcagtgtcgc caccgcgtta ctgttacgtg
    18361 catcgcaggc aagatgaaat ccgccaatcc ctttgatggc gacaatgttg cccattttta
    18421 actgtgcgat agctgcctgt aatgccgcct cttgttctgc atgttcacca tgacttaccc
    18481 attcaagatg cgggccacac tccgggcagg ccaccggctg ggcgtggaag cgacgatcga
    18541 gcgggtcgcg gtactctttg tcacaggccg gacatagcgg aaacgccgcc atcacggtaa
    18601 acgggcggtc gtaaggcatc gcgcgaataa tggtgaaacg cgggccgcag tgggtacagt
    18661 tgataaacgg ataacgataa cgccgctcgc ctggggtatt catttcggca aggcaagcag
    18721 ggcaagtggc ggcatccggg acaatttgcg tattcatggt gccgccggtg ctctggcgga
    18781 tggtgaactc ggtgggcagt tgtgaccaga taaacggctc acgctcgacg ctatcaatac
    18841 gcgccagtgg cggacagtgc tgatgcaatt gaacaagaaa cgtttccgga tcttccagca
    18901 gccggacttc tacgccatcg ccgtcattac atacatcgcc gtgaagattt aattgctgtg
    18961 ccagctgcca gacaaacgga cgaaaaccga cgccctgcac tttgccacga atacgcagtt
    19021 ggacaccgca agatgtgttt tttgccattg agttattccc gccatcatga attgcgtaac
    19081 ccgccctgcc ggacacgaca gcgtcgtatc cggcagtctc aggtcggcga taccgcccgc
    19141 tccgtattct acgaatattt ccgggaattc ctttgatgcc agaacagttc tgtaagattt
    19201 ttagaacatc agcgccgtac ggcggcgttt ttctgcgctc agttgttcaa gtttattacg
    19261 atcgacacaa atcagcgcat gggtcgggca agccgccata cacgccgggc cgtcttcacg
    19321 atggttgcac aggtcgcatt tattggcttc ggctttgtca gcccgtacat tcagacccgc
    19381 gccgctgttg cgaatcacag gacgtaccac cacttccatc gcgccatacg ggcaagccac
    19441 cacgcaggtt ttgcaaccaa tgcaacgttc ctgcatcaca tgaacaaacc ctttatcacg
    19501 gctgatagca ccattcgggc agacgttagc gcacggtgca tcttcacact gacggcaaac
    19561 tgtcgccgtg gaaatgttca cacctttaat gacatggata cgcggtaaaa atgtttccgg
    19621 ggtcagcgat gcacagtcct gattttcctg atgagaaacc acgcacgcca cttcacaggt
    19681 acggcaacca atacatttac tcgcgtcagc aatgatgaaa cggttcatca aattctccag
    19741 caatgacagt taatgcgccg atacattcac aaatcatgcc agtttttaat ttgctgttat
    19801 ttaaggaaat taatttctgt aatgcaggaa aaaagatgtc atcgacacta gtgacgatga
    19861 catgtgatga caatgtttat cgcgaaaggg caatgagtga gtcgcggcgg atcagttttc
    19921 cgctaaaggt tttcggcggt gagaaatccc cgccatcgag cataaaaatc agccgtccaa
    19981 taatttcctg aatcatctca gtcaccggaa tttttacgct ggagagcgcc ggaacgatat
    20041 agggggcaat tgcgatatca tcgaatccga taactgacac ctgctctggc accgctacgc
    20101 cgcgctcgtg caacgctttg atcgcaccta tcgccatatc gtcgttactg gcaactaacg
    20161 cgctaaattt agccccacgt tcgagcaacg tttctacccc ttcggccccg ctggcgggcg
    20221 tccatttacc gttagcgata agtttttcat tgagcgcaat accatgctgc gacagcgcgt
    20281 ctttataccc ggcaagacgt tcaatgctgg tgggggaatc catcgagccg gtaaggaaag
    20341 caatctcctg atgcccggcg tttatcaact ctgccacggc gttaaagctg gtctgtttat
    20401 gatcgcacca gacgctatgg ctgctattat tgcgcaggcg gcgattaagc accattatcg
    20461 gctgactgtg cgcgtcaatg atgtcatcaa tctcatccac gcttaaaaaa cgcgggtaaa
    20521 tcataatggc gtcgcagcgc aaatccagca gatactgaat cgcctggcgc tcttcttcgg
    20581 cgctgtgttt accatctgcc aatagcaact gtcgcccttt ctcttccgcc attcgcgcgg
    20641 catgaaacag taattcacta aaataaatgc cgtgataaag cgtgttggtc actaccagcc
    20701 ccagcgtctg agtactcttc gccgacagat tgcgggccag caaatttggg cggtaactgc
    20761 tctcttctac cgcctgaaac acgcgatctt tagtctcctg gctgacgtag ccattacctg
    20821 aaagcacgcg ggaaacggtc gcttttgaaa ccccggcgcg cttcgccact tccagcatcg
    20881 tcgtcatcat tttcatccct ttacacgcaa tcaacgcagt gtactgcacc gtttgccgat
    20941 tgtccttgcg caatcggcgg gaaaaatatt caggtgaccg gtttcacaaa tataaaaaat
    21001 gaacaattaa ctctcttgct tatttagtga caactattca tgattttgtg aaaccggttt
    21061 cttaattccg tttcagcatc ggtatttttc cgtcacgtcg actgataaca actacatcta
    21121 ccctactgat aacaggataa aatccgatgg ccaaaaatta tgcggcgctg gcacgctcgg
    21181 tgatagcggc actgggcggc gttgataaca tctcggcagt cacgcactgt atgacgcgtt
    21241 tgcgctttgt tatcaaagat gaccagctta tcgacagccc gacgttaaaa accatccccg
    21301 gcgtgctcgg cgtggtacgt agtgacaacc agtgtcaggt aattatcggc aatacggttt
    21361 cgcaggcctt tcaggaagtc gtcagcctgc tgccgggaga tatgcagccc gcacagcccg
    21421 tgggtaaacc caaactcacg ctgcgtcgca ttggtgcggg gatcctcgat gcgctgatcg
    21481 gcaccatgtc accgctgatc ccggcgatta tcggcggatc gatggtcaaa ctgctggcga
    21541 tgatcctcga gatgagcggc gtgctgacaa aaggatcgcc gaccttaacc attctgaatg
    21601 tgattggtga cggtgctttc ttcttcctgc cgctaatggt cgcggcatct gccgccatca
    21661 aatttaaaac caatatgtcg ctggcgattg ccatcgcggg tgtgctggta catccgagtt
    21721 ttattgaatt aatggcgaaa gcggcccagg gtgaacatgt cgaatttgcc ctgatcccgg
    21781 tcaccgcggt gaaatacacc tacacggtga tcccggcgct ggtgatgacc tggtgcctgt
    21841 catatatcga acgctgggtg gatcgcatta caccggcggt gaccaaaaac ttcctcaagc
    21901 caatgctgat tgtgttgatt gccgcaccgc tggcaatcct gctgattggc ccgattggta
    21961 tctggatcgg tagcgccatt tcggcgctgg tttacaccat tcatggttat ctgggctggc
    22021 tttcagtcgc cattatgggc gcgctgtggc ctctgctggt aatgaccggg atgcaccgcg
    22081 tctttacgcc aaccatcatt cagaccattg ccgaaaccgg caaagaagga atggtcatgc
    22141 catcagagat cggcgctaac ctgtcgctgg gcggttcatc actggcggtg ccgtggaaaa
    22201 cgaaaaaccc ggaactgcgc cagacggcgc tggctgcggc ggcatcagcc attatggcgg
    22261 ggatttccga accggcgtta tacggcgtgg cgatccgcct gaaacgtccg cttatcgcca
    22321 gtcttatcag cggttttatt tgcggcgcgg ttgccggtat ggcggggctt gccagccact
    22381 caatggcagc gccggggcta tttaccagcg tgcaattctt cgatccggcg aatccaatga
    22441 gcatcgtctg ggtgttcgcg gtcatggcgc tggcggtggt gctgtcgttt atcctcacac
    22501 tgttgctcgg ctttgaggat attcctgttg aggaagcggc tgcccaggcg cgaaagcatc
    22561 agagcgtaca accgaccgtc gccaaagaag taagtcttaa ttgaggatga aaatgtcagt
    22621 atttccagaa ggttttttat ggggcggcgc gcttgccgcc aaccagtctg aaggtgcgtt
    22681 ccgtgaaggc ggaaaagggc tgaccaccgt cgatatgatc ccacacggcg agcatcgaat
    22741 ggcggtgaaa ctggggctgg aaaaacgttt tcagttgcgc gatgacgagt tttatcccag
    22801 ccatgaggcg acggattttt atcatcgtta taaagaagat atcgccctga tggcagagat
    22861 gggattcaag gttttccgta cctcaattgc ctggagccgt ctctttccgc aggacgatga
    22921 aatcacgccc aatcagcagg gcattgcttt ttatcgtcct gtctttgaag agtgtaaaaa
    22981 gtacggtatc gaaccgctgg tcacgttgtg ccacttcgat gtgccgatgc atctggtcac
    23041 cgaatatggc tcctggcgta accgcaagct ggtggagttt ttcagccgct acgccagaac
    23101 ctgctttgaa gcatttgatg gtctggtgaa atactggcta accttcaatg aaatcaacat
    23161 tatgttgcat agcccgttct ccggcgcggg tctggtgttt gaagaaggtg aaaatcagga
    23221 tcaggtgaaa tatcaggccg cgcatcacca gctggttgcc agtgcgctag ccaccaaaat
    23281 cgcccatgag gttaacccgc aaaatcaggt gggctgtatg ctggcgggcg gtaacttcta
    23341 cccttacagc tgcaaaccgg aagatgtctg ggcggcgctg gagaaagatc gggaaaacct
    23401 gttttttatc gatgtgcagg cgcgcggcgc gtatccggct tactctgccc gcgtatttcg
    23461 cgaaaaaggg gtaaccatag ataaagcgct gggcgatgat gagatcctga aaaacaccgt
    23521 cgattttgtc tctttcagct attacacctc gcgctgcgcc tcggcggaga tgaacgccaa
    23581 caacagcagt gcggcgaacg tggtgaaatc gctgcgtaac ccgtatctgc aagtgagcga
    23641 ctggggctgg ggaattgatc cactcggtct gcgtatcacc atgaatatga tgtacgatcg
    23701 ttatcagaag ccgctgtttc tggtggaaaa cggcctcggc gcaaaagatg aacttgctgc
    23761 caatggcgag attaacgatg actatcgcat cagctattta cgcgaacata tccgcgcaat
    23821 gggcgaagcg attgcagatg gcattccgct gatgggctac accacatggg gctgtattga
    23881 tttagtttcc gcctctacgg gtgaaatgag caaacgctac ggttttgtct acgtagaccg
    23941 tgacgacgca ggcaacggca cgctgacgcg cacacgtaag aaatcgttct ggtggtataa
    24001 aaaagtgatt gccagtaatg gggaagattt agagtaagta acagtgccgg atgcggcgtg
    24061 aacgccttat ccggcctaca tagttcgtgc gacatgtagg cccgataagc gcagcgcatc
    24121 gggcaattta gcatttggca ttagtcaaaa atcaaagaaa ccattccggc ttaacgccaa
    24181 atcgggctga aagtgcctta atgtgggata tcgtcaggga tcgttgtcca gacaaaatct
    24241 gactgaccag ggatttagag ccgatttcgt tcttcaaatc cgcataggtc agattatgtt
    24301 gatcaatcag cgtgcgaagt aacgccacgc caacaggcat tgccgcaacg gctttgtcga
    24361 actctgcaaa ctcttggttg ttgttttcat attctgcaat gcggctcgcc agaaagtcga
    24421 taagtggatt ggtgtcatct tcttcaatca aatattcgac cagttccagt gcttcacgat
    24481 aatcatcttc tgagtcacta cctcccagaa aggggactgc attgaccagt tctcttgttg
    24541 ctttaacagc gtgtgcagca ttagcagtca ttctttgttc tctctgtagt aacgtgtcag
    24601 tttgtcatac tcggcgtgat tagttatgtg cttcacaaag aaccgcttat taacaaagtt
    24661 tatgtaggca atcaccccta ggttgttccc acctacatcc agaacgtacc atttgttgcg
    24721 atatttaaaa ttgtcgaggt tgggaaacgc cgttcgcatc tcttctggcg tagaaaaatc
    24781 cgtttcctta ataacccgat acaatgcctg aagagccaga gcgtcgttag gatatttacg
    24841 cgcagactct tcaaatggcg ctttagaaat gatgtgcatt tttaagtttt aacttcctta
    24901 tagtaagtaa acagccacat ctgcgagttc tatccttatg tgttcggaaa ctatagctca
    24961 tcaaatccaa gttcacaata tgtaaacact tgtgcgcgat ataattaccc ccaaaatcct
    25021 catagccact ctgcaaaaag tcagttctgg ttcacccctc aagcacatcc ccctttatca
    25081 agcaacgctg cgtctgcgcc agatcttccg tttgcgggtc gcgcccggcg atgggtttga
    25141 ggacgagttt acggttgggg gtataagtaa aacgcgtgag cgcgggatag cttaacttgt
    25201 caaaccagcc gtgaacacgc tgccatttgg cgtcttttac ctgatacaga ctggttatcc
    25261 agtaaccaga attaaactgc atatccagca attgcgtgtg accattgcct tgcaaatcca
    25321 gcaaatcatc tacgccagtt ttgcttgcgg tgtagaatcc tctgggttca aagacacagg
    25381 gacggccttc gcgcgtgaac gtcataagaa ttaaatgtgc ataaggagcc agaccattac
    25441 cggtacttgg taaccagata accagatcct gaatgccgtt acgatcaaga tcggcgcgat
    25501 agagttgccc tttgcccgct atgtttgcca gcatacccac aggcacagtc caggatttac
    25561 cggcgcggtc tttagcggtt aattgccagt aatcttcttc atttggcatc ttctcaagcg
    25621 tcacgccagc aacgcccact ttcctaaaat gccaggtttg ctcggcatca taaggcatgg
    25681 tcaggagagg tttcagcgcc cgcttttgcg cgtattcgct gtcactcatt ggcagatatt
    25741 cggtaaagct ggtggcctgt gcaggcagca ctaagctcaa taaaaaagcg cacatccctg
    25801 ttctgatgtt caattccctg aattccctga attccctgat ggagaattat tcttcttcca
    25861 ccgctaactg cgcaaatcca ccatttcctt cccagccttc cagtcgttga taaacggttt
    25921 ctaccgcatc tttaatcggc tgggtcatcg ggtagtaaaa gccgacgata tccggctgaa
    25981 tgccgaggaa aatcacttca ccaatgtctt cttttaactg gtcgataagg taattcaacg
    26041 gcatgttatg ggtagtcatc ataaacatct cggcgatatc atccgggtcg atgatgcgga
    26101 tttcgccggg gtttagcccc atatccgtgg cgtcgacaat cagcagtcgt gtcgggcgca
    26161 gttcacggat ggcgacgatg tcgttttccg gtgcactacc gccgtcaatc accacccagt
    26221 tacctttcgg cgcggcggcg cacttttccg ccagcagcgg gccagcgcca tcatcgccca
    26281 tcatgctatt gccaacacag agtaaaacgt cagtcacgca gtctcctcac catcaaatag
    26341 atggcgtttt cctgatgaat atcatgcagc atacttaaca gcattgtact ccattcctgc
    26401 tgctgcgaag tttgcgctgc ccgggcttta tcgaatgcgt tggccagcat cggcacatgg
    26461 ttaatgtcga tgacgatctc gccatatttc ggtacgcctt ccattttgtg gcgggcttcg
    26521 ctgcccgcct ccagagtggc aatccatgcc agatattcat cccatgggca ggtgagcgcc
    26581 gcttccaggc aatcgataac cccaaggtgg tgaccaatcg ccaggctgta atagaccacc
    26641 tgctgcgcct cggcgggcgt ggcatcgttc tcatcaataa atttacggct cagttgactg
    26701 aacaccacct tttcactcat cggatacgcg cctcttcaac aacatgattc agatggctga
    26761 caatctcgtt cagacgcgga tcgttttccg cttccagcca gcgtgcaacc tgttcttcgc
    26821 cctgccctaa ctgtgtaagg taatcatcgg caatctgacg accgtaacga taacccgcca
    26881 ggcgacgtgc ttcgcgatcc actttcacgc gcagcggctg caccatatcg ccatgcagga
    26941 tctccgccgg ttgttcatcc agttcacccg gcccacgggc gtgaattttc tgctccagca
    27001 ggccgagcgc cattgcaaag ccgtacagcg tggcggcagg cgttggcggg cagctaggga
    27061 tataaacatc cacagggaca attttatccg taccgcccca cacgcagtag agatcgtgta
    27121 agatcccgcc gctgttaccg caggcaccgt atgagataca gattttcggg tccggcgcgg
    27181 actgccacgc acgcagcgca ggggatcgca ttgcacgggt gaccgcgccg gtaaacagta
    27241 aaatatccgc atgacgcggt gaaggaacga ctttaatgcc gaagcgttct gcatcgaaca
    27301 gcggagaaag cgtggcgaaa atttcgattt cgcagccgtt gcagccgccg cagtccacgc
    27361 ggtaaacata ggcagaacgt ttgatttttt tcagtaacga cgccttcatg ctggcgatgg
    27421 attcatccac cgtcatcggg accggaatgc cgttggcgtc acgggggcct aataaattgc
    27481 tcatcagatg gcctctttca tatggcgagt cagttcaata cggtcggacg gcaccaggca
    27541 tttctggcgc ttacattccg ggcaagtctc aaagctttcg cggtggtttt ccgcgcggct
    27601 gtcaccattg tgcttaagca gcgcaatggc gtagtcgatc tctttctgta cggcgaaagg
    27661 acgattgcag acgcggcagt tgcacagcgc gaagcgggac tgttgcagga agtcttcttt
    27721 cttccacacc gccagttcgt actcttgcga cagtttgatc gccgccgtcg ggcaaacttc
    27781 ttcgcagcgt ccacagaaga tgcagcgccc gagattaaac tcccacgcca gctctcctgt
    27841 ggcgaggtca gtttcaaccg ttaaggcgtt tgacgggcag gcattgacgc aagccgcgca
    27901 gccgatgcac tgctgcgggt tctgctctgg cttaccacgg aagtttttat caaccgcaat
    27961 cggctccagc ggataagacg aggtcgccgt gccggttttg atgacttttt tgataaaggt
    28021 aaacatggcg attccttatt tcagcggcga gtttttacgc tcaatgctgt aacgctcgag
    28081 ttctttgtac ggcaccactt tgctcttctt cttacgcaca tcgactacgg tcatgcggtc
    28141 ggtacaggag tagcaagggt ccaggctacc gataatcagc ggcgcatcgg aaacggtatt
    28201 gccacgcagc atgtagcgca gggtcggcca gttcgcgtag gtcgcggcac ggcagcgcca
    28261 gcggtacagc ttctggttgt cgccggtcat gctccagtgg atatcgtcgc cgcgcggcgc
    28321 ttcggcaaag cccagcgcga aacggtgcgg aatgtaggta aagccttcca ccatcagcgg
    28381 accgcccggc aggttatcca gaccgtagtc gatcatattc agcgcggtat agacctcgtt
    28441 gattcgcact ttcagacggg aaataacgtc gcagccctgc tcgctgtgga cttccattgg
    28501 cagcaggcca taaccgacaa acgggtgatc ggcgcgagta tcgcgggcga gaccgctggc
    28561 gcgaaccatc gggccaacgt tactgaaatc gcaggcgatt tccgggtcca gacgaccaat
    28621 gccgacggtg cgttgttcca tgttcggcgt actcagcagc acatccacca gctcctgcac
    28681 ttcacgacgc atttgttgcg ccagctggcg ggtctggatc atgtcatctt tcagcagatc
    28741 gtgacgaatc ccgccgatca ggttcaggcc gtaggtttta cgcgcaccgg taaggatctc
    28801 tgccattttc atggaggttt cacgcacgcg gaagaactgc ataaagccgg agtcgaagcc
    28861 ggtaaagtga caggcgaggc cgaggttgag cagatgcgag tgcaggcgtt ccacctccag
    28921 cagaatggcg cggatcatct gcgcgcgttc tggcacctga atacccatcg cgttttccac
    28981 cgacgtggtg taggcggtgc tgtgggcaaa gccgcagatc ccgcacacac ggtcagagag
    29041 gaaggtcact tcgttataac ccatgcgggt ttccgccagt ttttccatac cgcgatggac
    29101 gtagaacaga cggtagtcgg cgtcgataat gttttcgcca tcgacgaaca gacggaagtg
    29161 acccggttcg tcagaagtga cgtgcagcgg accaatcggc acgacgttgt ttttcttgtc
    29221 gcccagttcg ttgatgaact cgtaggtttc agcatcggta gtcggtgccg gacgctgacg
    29281 ataatccatg ctgtctttac gcagcggata aagttcatcc ggccagtcat ccggcagacc
    29341 aaccggaatc aaaccgtaca tatcgcgcac ttcgcgctcg ccccacaccg ccgccggaac
    29401 gcgcggcgtc acggacggat actccggttt gttggcgtca acttcgacgc gaaccgttac
    29461 ccaacacttg gtgcccttct ccatcgacag cacgtaataa acggcgtaat gaccattcag
    29521 tttgcgttcg tcgttaccaa acagcaccga cagccagcca ccctgcttgt agtagagaaa
    29581 ctccaccact tccggcagat agttcacctt tacggtgaca gtcagctgat ctttggtctg
    29641 ccaggcgtgg tccagcacga cgcctggaaa tgcctcattc agcgcggcga gataatgttg
    29701 acctaatttt tcttcagaca tgctcaaact ctctttaatc acgccgccag caaggagacg
    29761 aacgctaaaa atgcaaagcc aaacccagcc caggtaatgc gcggagtaat atcaagacgc
    29821 agacgggcca tactgttttc gaacagcgcg ataaccagga cgccgaccac cagtttgacg
    29881 atggcaatca ccagcgccag cagcagtcca ccgacggtga aggtttccat ttgtccccac
    29941 ggaataaaca ccccgacgaa catctgcaac accaccagct gtttcaggct gataccccat
    30001 ttcatgacgc caaagccgct accgctgtat tcggagagcg gaccttcctg caactcctgc
    30061 tcggcttccg ccaggtcgaa cggcagtttg cccatttcga taaaagtggc gaacgcacag
    30121 gcgcagagcg ccagcaccag cgggatgctt tggctcagcg gccagtgata aacggtgtcg
    30181 gtaatgttgc tgatgttggt ggaaccggca acctgtgcgg caacccacag accaagcagc
    30241 aacatcggtt cgaccagcac gccaagcatc gcttcacggc tcgcaccgat agcggtaaac
    30301 gggctaccgg tatccagacc ggaaatggca aagaagaaac gcgcgatggc gaagagatac
    30361 agtaaggtga tcaaatcgcc cagttgcggc agcggagaac cgatagtcag catgacgccc
    30421 accatcacat acggcgtcag gcggaacatc cagccagagg catccgggcc aacgctctgg
    30481 cggcccagca gtttgataat gtcgcgatac tcctgcaaca cgcccggccc gcgacggtta
    30541 tgcagacggg cgcgcgccac gcgggtaata ccggagagca gcggcgcaac ggcaaataac
    30601 accagcgcct gaattaacgg atataaaaca ctcattctca ggctcctcgt gaaacaataa
    30661 tcaccaccag taccgccagt tcaaccagcg ccatccggcg gaacagcaac gcactacctt
    30721 cgcactgcca gcccggcacc agcgacaccg gattcagcca tttgcgcagt ttcagcactg
    30781 gcgcaaacgc ctgtttaacc ggcatggcaa aaccgtgagc ggtaatcacc attgattttt
    30841 catggtcgta gccgcacacc caggccgcac cgcgggaacg tgacggcagg cggtcgcctt
    30901 tgcaaatcgc cataatgatg aatggcagca gcgggcaggc aatcagcagc aatgtgatca
    30961 ttggttgaga aacggtggtg ttagcaggct ccagaggcag aggtacagca gcagagagca
    31021 tcggcagcag ccacggcgca gcaacaccgc caattacgca gcaaatcgcc agtgccacta
    31081 cgcttacgct catcaggagc ggcgcacagg tggcgttttc ggcttctttg gtacgcggcg
    31141 cgccgaggaa cgtgacgcca tagactttcg ccatacacat caccgccagc gcaccggtaa
    31201 ttgccagacc cacagcgagc agcggcccca gcagacgggc aacaaacgcg ccgctattgc
    31261 tcagtttgaa gaaggattga tagataaccc attccccggc aaacccgttc agtggcggca
    31321 gcgcagccat tgccatcaac ccgactaaca tggcgatgga gataaccggc attttcttgc
    31381 caataccacc gagtttttcg atatcgcgat gaccggtacg gaaccagacg ctccccgccc
    31441 cgaggaacag tacgcttttg aacaggctat ggttaagcag atggtacaga ccaccgacca
    31501 ggccaagagc aatcagcgcc ggttgttcga gcgcgatacc cgttacgcca gcgcccagcc
    31561 ccagccctag ccctagccct agccctagcc ctagccctag ccctagcagg ataatgccga
    31621 tattttccag ggtgtggtaa gccagcaggc gctggatgtt gtgttccatc aacgcataca
    31681 gaccaccgac gaacgcggtg atcatgccga gcaccagcag cgcgatcccc caccacagcg
    31741 gtgcattacc gcccagcagt gacagggtta acatgcccag caggccaatt ttcatgacta
    31801 ccgtagaaaa caacgcggca gctggcgcag aggcgttagc atgtgcctgc ggcacccagc
    31861 cgtgcagcgg aataatccca gccagcaggc caaagccaat cacaccgagc agccagatat
    31921 cggaaccgag cagccagata tcggaaccga gcggcagctg ttgcatacgc atatccagca
    31981 ggcgcagatc cagcgtgcca taacgctgcc acagcagcca gcaagcaatc accagcagca
    32041 gagtgccaag acgccccagc gcaaaccaca gtttgccctc tttgctgttg ctggtgagga
    32101 acaccgcaca caaggccatg acttcggcca ttaccacgaa catgccgagg ttgctggcga
    32161 tgacggcgca gacggcggca gccatcaaca tattgatctg caagccgttg cacttcacct
    32221 gcgcgtggcg atgccagtca atgttataga ggctgacaaa cagaccgcaa aggccgaggg
    32281 taatcagcca aatcgcgtta agcggggaga tttgcacatc gtagcttacc agcgacatcg
    32341 caccgctcac gccaaccgag ccagtcagta cagtgaagcc cgcggctgcc gtatacagac
    32401 taccaaccgc gccgccaatt ccggctatcc agccacttag cgctttttga aaagaaaaga
    32461 gaaatgccag aacagcggcg gcgacaaacc atgccacgcc gctattgatc agggaaattg
    32521 cgctcattta gcctctccac tttgagcctg ctgaaacaag gtgagatcgc caaagtccgt
    32581 gttaaaagtc agctcacgct tacgtttgct gacgcgggcg atatcggtgt tatccaccag
    32641 atgcagggct ttagtcgggc acatccgcac acaggccgga ccttgttcat caaagctaca
    32701 aaggtcacat ttgacggcga tcgcgcgaat acctgacacc cagtcaagca atgtgctgac
    32761 acgcgccgga gcaggcggtg ccggtggcgc tttcggggta ttggcgtttg ccggaatatc
    32821 cagcggacgg ctgccggaaa attcaattgc gccaaacggg caggcgatgc cgcacagttt
    32881 gcagcttacg cacagacttt cattcaactg cacggcccca tcaacgcggg tgatggcgtt
    32941 aaccgggcag accaccgcgc agggtgcatc ttcacagtgg tgacagagca gcggcgcaga
    33001 ttctttttca ttgagcatca ctctcaggcg cggcattgat tgcaggccgt gctggcgatg
    33061 cgtctctgaa caggcggcct cacaggtgtg gcagccgata cagagcgtgg agtcggcaat
    33121 tacaaaacga ttcaccaggc attcctcagg tgattgtcat ttttgacgaa aacatgccga
    33181 tgaaatgtca ttttcgacac tcatcgacac gcccatcccc aaacaggcgt aacgcctgca
    33241 aaacgggcaa agcctcagct catgctgccg ggctttgtcc ctttaccagt tggcttaaat
    33301 tcaccggcac attgttttca atggcggtgt ataagctgtc gtaaacgcca gcgatttttt
    33361 cgagatagtg ttccagaacc tgttcgcgat cgcggatgtt gaccttgcca tcaatcatgc
    33421 cgtcaatgct cagttgcgcc tgcgcaatcc gctgtttgtc ttcgccatct tttgtttcga
    33481 cgtaatactc gatggtgtgg ctgttaaagc catccgccag ggtgacgtaa atacgaaaat
    33541 gttcaaaaag gacgaaacag agttctttgt ccggcgcgca gctcatggcg tgatgcaggc
    33601 gcgctttcga taacgtgatc ccctgaacca gcgaattgca gtagatgtgc cactggtcct
    33661 gtaggcgacg atgccgctgt gcgatgtaat cggctttctc gcttatttcc caaatagtca
    33721 ttgtcaggtt actcgtttaa cagagatgcc agctttaagc attttttgta ccaactttta
    33781 atttattgtt attaaagaga tttttaagct aaagatgaat ttcgtcgccg tgtcgacgtg
    33841 tcatttcgac atcatcgaca ttattcaccg cagggataat caacactggc gcaattattg
    33901 cttgtagctg gcaatagtta atgggaggcg atatgcacga aataaccctc tgccaacggg
    33961 cactggaatt gatcgaacag caggccgcaa aacacggcgc aaaacgcgta actggggtct
    34021 ggctcaaaat tggcgcattt tcttgtgtcg aaaccagctc tcttgccttt tgttttgatc
    34081 tggtttgccg cggcagcgtg gcggaaggtt gtaaactgca cctcgaagaa caagaggccg
    34141 aatgctggtg tgaaacatgc caacagtatg tgacgctact gacccagcgc gtccgccgct
    34201 gtccacagtg tcatggtgac atgctacaga ttgtggcaga cgacggttta cagattcggc
    34261 ggatagaaat agaccaggag tgagcgatgt gtacaacatg cggttgcggt gaaggcaacc
    34321 tgtatatcga gggtgatgaa cataaccctc attccgcgtt tcgtagcgcg ccatttgccc
    34381 cggcggcacg cccgcagatg aaaatcaccg gcattaaagc gcctgaattt acccccagcc
    34441 agactgaaga aggcgacctg cattacggtc atggcgaagc gggcactcac acaccgggca
    34501 tgagccagcg tcggatgctg gaagtcgaaa ttgacgtgct ggacaaaaac aaccgtctgg
    34561 ctgaacgcaa ccgcgcgcgc tttgctgccc gcaagcaact ggtgctcaac ctggtttcca
    34621 gccctggttc cggtaaaacc accctgctga cggaaacctt aatgcgcctg aaagacagcg
    34681 ttccgtgcgc ggttattgaa ggcgaccagc aaaccgtgaa cgatgccgca cgcattcgcg
    34741 ctaccggtac accagcgatt caggtgaaca ccggtaaagg ctgccatctt gacgcacaga
    34801 tgattgccga cgccgcaccg cgtctgccac tggacgataa cggtattctg tttatcgaaa
    34861 acgttggcaa cctcgtatgc ccggccagct tcgatctcgg tgaaaaacac aaagtagcgg
    34921 tgctttccgt taccgaaggt gaagacaaac cgctgaaata tccgcatatg tttgccgccg
    34981 cctcgctgat gctgctcaac aaagttgacc tgttgccgta tctcaacttt gacgttgaga
    35041 agtgcatcgc ctgcgcccgc gaagtcaatc cagaaattga aatcatcctt atttccgcca
    35101 ccagcggcga agggatggac cagtggctga actggctgga gacacagcga tgtgcatagg
    35161 cgttcccggc cagatccgca ccattgacgg caaccaggcg aaagtcgacg tctgcggcat
    35221 tcagcgcgat gtcgatttaa cgttagtcgg cagctgcgat gaaaacggtc agccgcgcgt
    35281 gggccagtgg gtactggttc acgttggctt tgccatgagc gtaattaatg aagccgaagc
    35341 acgcgacact ctcgacgcct tacaaaacat gtttgacgtt gagccggatg tcggtgcgct
    35401 gttgtatggc gaggaaaaat aatgcgtttt gttgatgaat atcgcgcgcc ggaacaggtg
    35461 atgcagttaa ttgagcatct gcgcgaacgt gcttcacatc tctcttacac cgccgaacgc
    35521 cctctgcgga ttatggaagt gtgtggcggt catacccacg ccatttttaa attcggcctc
    35581 gaccagttac tgcctgaaaa cgttgagttt atccacggtc cgggttgccc ggtgtgcgta
    35641 ctgccgatgg gtagaatcga cacctgcgtg gagattgcca gccatccgga agtcatcttc
    35701 tgtacctttg gcgacgccat gcgcgtacca gggaaacagg gatcgctgtt gcaggcaaaa
    35761 gcacgcggtg ccgatgtgcg catcgtttac tcgccgatgg atgcgttgaa actggcgcag
    35821 gagaatccaa cccgcaaagt ggtgttcttc ggcttaggtt ttgaaaccac catgccgacc
    35881 accgccatca ctctgcaaca ggcgaaagcg cgtgatgtgc agaattttta cttcttctgc
    35941 cagcacatta cgcttatccc gacgctgcgc agtttgctgg aacagccgga taacggtatc
    36001 gacgcgttcc tcgcgccggg ccacgttagt atggttatcg gcactgatgc ctataatttt
    36061 atcgccagcg attttcatcg tccgctggtg gtcgctggtt tcgaacccct tgatctactg
    36121 caaggcgtgg tcatgctggt ggagcagaaa atagcggccc acagcaaggt agagaatcag
    36181 tatcgtcggg tggtaccgga tgccggtaac ctgctggcgc aacaggcgat tgccgatgtg
    36241 ttctgtgtca acggcgacag cgaatggcgc ggcttaggcg tgattgaatc ttctggcgtg
    36301 cacctgacgc cggattatca acgattcgat gccgaagcac atttccgccc ggcaccgcag
    36361 caggtctgcg atgacccgcg cgcgcgttgt ggtgaggtat taacgggcaa atgtaagccg
    36421 catcaatgcc cgctgtttgg taacacctgt aatcctcaaa ccgcatttgg tgcactgatg
    36481 gtctcctccg aaggagcgtg cgccgcgtgg tatcagtatc gtcagcagga gagtgaagcg
    36541 tgaataatat ccaactcgcc cacggcagcg gcggccaggc gatgcagcaa ttaatcaaca
    36601 gcctgtttat ggaagccttt gccaacccgt ggctggcaga gcaggaagat caggcacgtc
    36661 ttgatctggc gcagctggta gcggaaggcg actgtctggc gttctccacc gacagttacg
    36721 tcattgaccc gctgttcttt cctggcggta atatcggcaa gctggcgatt tgcggcaccg
    36781 cgaatgacgt tgcggtcagt ggcgctattc cgcgctatct ctcctgtggc tttatcctcg
    36841 aagaaggatt gccgatggag acactgaaag ccgcagtgac cagcatggca gaaaccgccc
    36901 gcacggcagg cattgccatc gttactggcg atactaaagt ggtgcagcgc ggcgcggcag
    36961 ataaactgtt tatcaacacc gctggcatgg gcgcaattcc ggcgaatatt cactggggcg
    37021 cacagacgct aaccgcaggc gatgtattgc tggttagcgg tacactcggc gaccacgggg
    37081 cgactatcct taacctgcgt gagcagctgg ggctggatgg cgaactggtc agcgactgcg
    37141 cggtgctgac gccgcttatt cagacgctgc gtgacattcc cggcgtgaaa gcgctgcgtg
    37201 atgccacccg tggtggtgta aacgcggtgg ttcatgagtt cgcggcagcc tgcggttgtg
    37261 gtattgaaat ttctgaatca gcgctgccgg ttaaacctgc cgtgcgcggc gtttgcgaat
    37321 tgctgggact ggacgccctg aactttgcca acgaaggtaa actggtgatc gccgttgaac
    37381 gcaacgcggc agagcaagtg ctggcagcgt tacattccca tccactgggg aaagacgcgg
    37441 cgctgattgg tgaagtggtg gaacgtaaag gtgttcgtct tgccggtctg tatggcgtga
    37501 aacgaaccct cgatttacca cacgccgaac cgcttccgcg tatatgctaa taaaattcta
    37561 aatctcctat agttagtcaa tgaccttttg caccgctttg cggtgctttc ctggaagaac
    37621 aaaatgtcat atacaccgat gagtgatctc ggacagcaag ggttgttcga catcactcgg
    37681 acactattgc agcagcccga tctggcctcg ctgtgtgagg ctctttcgca actggtaaag
    37741 cgttctgcgc tcgccgacaa cgcggctatt gtgttgtggc aagcgcagac tcaacgtgcg
    37801 tcttattacg cgtcgcgtga aaaagacacc cccattaaat atgaagacga aactgttctg
    37861 gcacacggtc cggtacgcag cattttgtcg cgccctgata cgctgcattg cagttacgaa
    37921 gaattttgtg aaacctggcc gcagctggcc gcaggtgggc tataccccaa atttggtcac
    37981 tattgcctga tgccactggc ggcgggaggg catatttttg gtggctgtga atttattcgt
    38041 tatgacgatc gtccctggag cgaaaaagag ttcaatcgtc tgcaaacatt tacgcagatc
    38101 gtttctgtcg tcaccgaaca aatccagagc cgcgtcgtca acaatgtcga ctatgagttg
    38161 ttatgccggg agcgcgataa cttccgcatc ctggttgcca ttaccaacgc ggtgctttcc
    38221 cgcctggata tggacgaact ggtcagcgaa gtcgccaaag aaatccatta ctatttcgac
    38281 attgacgata tcagcattgt cttacgcagc caccgtaaaa acaaactcaa catctactcc
    38341 actcactatc ttgataaaca acatcccgcc cacgaacaga gcgaagtcga tgaagccgga
    38401 accctcaccg aacgcgtgtt caaaagtaaa gagatgctgc tgatcaatct ctacgagcgg
    38461 gacgatttag ccccctatga acgcatgttg ttcgacacct ggggcaacca gattcaaacc
    38521 ttgtgcctgt taccgctgat gtctggcgac accatgctgg gcgtgctgaa actggcgcaa
    38581 tgcgaagaga aagtgtttac cactaccaat ctgaatttgc tgcgccagat tgccgaacgt
    38641 gtggcaatcg ctgtcgataa cgccctcgcc tatcaggaaa tccatcgtct gaaagaacgg
    38701 ctggttgatg aaaacctcgc cctgaccgag cagctcaaca atgttgatag tgaatttggc
    38761 gagattattg gccgcagcga agccatgtac agcgtgctta aacaagttga aatggtggcg
    38821 caaagtgaca gtaccgtgct gatcctcggt gaaactggca cgggtaaaga gctgattgcc
    38881 cgtgcgatcc ataatctcag tgggcgtaat aatcgccgca tggtcaaaat gaactgcgcg
    38941 gcgatgcctg ccggattgct ggaaagcgat ctgtttggtc atgagcgtgg ggcttttacc
    39001 ggtgccagcg cccagcgcat tggtcgtttt gaactggcgg ataaaagctc tctgttcctc
    39061 gacgaagtgg gcgatatgcc actggagtta cagccgaagt tgctgcgtgt attgcaggaa
    39121 caggagtttg aacgtctcgg cagcaacaaa atcattcaga cggacgtacg tttaattgcc
    39181 gcgactaacc gcgatctgaa aaaaatggtc gccgaccgag agttccgtag cgatctctat
    39241 taccgcctga acgtattccc gattcacctg ccgccactac gcgagcgtcc ggaagatatt
    39301 ccgctgctgg cgaaagcctt taccttcaaa attgcccgtc gtctggggcg caatatcgac
    39361 agcattcctg ccgagacgct gcgcaccttg agcaacatgg agtggccggg taacgtacgc
    39421 gaactggaaa acgtcattga gcgcgcagta ttgctaacac gcggcaacgt gctgcagctg
    39481 tcattgccag atattgcttt accggagcct gaaacgccgc ctgccgcaac ggttgtcgcc
    39541 caggagggcg aagatgaata tcagttgatt gtgcgcgtgc tgaaagaaac caacagcgtg
    39601 gttgccgggc ctaaaggcgc tgcgcaacgt ctggggctga aacgcacgac cctgctgtca
    39661 cggatgaagc ggctgggaat tgataaatcg gcattgattt aactgcaaat tgccggacag
    39721 atctgcctgt ccggcatact attcacgagg ttttttcgga cgatattttt ccggcagttc
    39781 tggcaccgga cgcttgtcat cgatgagatg acgcacggtt aagatcggat gacgccacag
    39841 cattctcggc ccggcccaac gcataatctg tttcatctct tcacgctttg caggctggta
    39901 acagtgcacc ggacactgct tacaggctgg tttctcttcg ccgaacacac atttatccag
    39961 ccgcttttgc gcgtaaacaa acaacgcctc gtaatgctcc ggctccgctg acgcctgcgg
    40021 gcatttcgct tgataaagat cgatcatttt tttaatcgtc agtttttcac gagagatacg
    40081 cttgccggac atgctgcctc cacctcatta agatgcattt atattacatc ttaatcttaa
    40141 agggcactat gactctaaag aagaagggtt cgccaatcga tacaattttg cgtacttgct
    40201 tcataaacat cacgcaaaag ctgcaaaaca gcatctttcc cggaaccagc atcaagaact
    40261 cgccgttcgc ttcttcccct gaaatgatta actccggtat catgtgcgcc ttatgtgatt
    40321 acaacgaaaa taaaaaccat cacaccccat ttaatatcag ggaaccggac ataaccccat
    40381 gagtgcaata gaaaatttcg acgcccatac gcccatgatg cagcagtatc tcaagctgaa
    40441 agcccagcat cccgagatcc tgctgtttta ccggatgggt gatttttatg aactgtttta
    40501 tgacgacgca aaacgcgcgt cgcaactgct ggatatttca ctgaccaaac gcggtgcttc
    40561 ggcgggagag ccgatcccga tggcggggat tccctaccat gcggtggaaa actatctcgc
    40621 caaactggtg aatcagggag agtccgttgc catctgcgaa caaattggcg atccggcgac
    40681 cagcaaaggt ccggttgagc gcaaagttgt gcgtatcgtt acgccaggca ccatcagcga
    40741 tgaagccctg ttacaggagc gtcaggacaa cctgctggcg gctatctggc aggacagcaa
    40801 aggtttcggc tacgcgacgc tggatatcag ctccgggcgt tttcgcctga gcgaaccggc
    40861 cgaccgcgaa acgatggcgg cagagctgca acgcactaat ccggcggaac tgctgtatgc
    40921 agaagatttc gccgagatgt cgctgattga aggccgtcgc ggcctgcgcc gtcgcccgct
    40981 gtgggagttt gaaatcgaca ccgcgcgcca gcagttgaat ctgcaatttg gcacccgcga
    41041 tctggtcggt tttggcgtcg agaacgcgcc gcgcggactt tgtgctgccg gttgtctgtt
    41101 gcagtatgcg aaagataccc aacgcacgac cctgccgcat attcgttcta tcaccatgga
    41161 acgtgagcag gacagcatca ttatggatgc cgcgacacgt cgtaacctgg aaattactca
    41221 gaacctggcg ggcggtgcgg aaaatacgct ggcttctgtg ctcgactgca ccgtcacgcc
    41281 gatgggtagt cgtatgctga aacgctggct gcatatgcca gtgcgcgata ctcgcgtgtt
    41341 gcttgagcgc cagcaaacta ttggcgcatt gcaggatttc accgccgagt tgcagccggt
    41401 actgcgtcag gtcggcgacc tggaacgtat tctggcacgt ctggctttac gaactgctcg
    41461 cccacgcgat ctggcccgta tgcgccacgc tttccagcaa ctgccggagc tgcgtgcgca
    41521 gttagaaact gtcgatagtg caccggtaca ggcgctacgt gagaagatgg gagagtttgc
    41581 cgagctgcgc gatctgctgg agcgagcaat catcgacaca ccgccggtgc tggtacgcga
    41641 cggtggtgtt atcgcatcgg gctataacga agagctggat gagtggcgcg cgctggctga
    41701 cggcgcgacc gattatctgg agcgtctgga agtccgcgag cgtgaacgta ccggcctgga
    41761 cacgctaaaa gttggcttta atgcggtgca cggctactac attcaaatca gccgtgggca
    41821 aagccatctg gcacccatca actacatgcg tcgccagacg ctgaaaaacg ccgagcgcta
    41881 catcattcca gagctaaaag agtacgaaga taaagttctc acctcaaaag gcaaagcact
    41941 ggcactggaa aaacagcttt atgaagagct gttcgacctg ctgttgccgc atctggaagc
    42001 gttgcaacag agcgcgagcg cgctggcgga actcgacgtg ctggttaacc tggcggaacg
    42061 ggcctatacc ctgaactaca cctgcccgac cttcattgat aaaccgggca ttcgcattac
    42121 cgaaggtcgc catccggtag ttgaacaagt actgaatgag ccatttatcg ctaacccgct
    42181 gaatctgtcg ccgcagcgcc gtatgttgat tattaccggc ccgaacatgg gcggtaaaag
    42241 tacctatatg cgccagaccg cactgattgc gctgatggcc tacatcggca gctatgtacc
    42301 ggcacaaaaa gtcgagattg gacctatcga tcgcatcttt acccgcgtag gcgcggcaga
    42361 tgacctggct tccgggcgct caacctttat ggtggagatg actgaaaccg ccaatatttt
    42421 acataacgcc accgaataca gtctggtgtt aatggatgag atcgggcgtg gaacgtccac
    42481 ctacgatggt ctgtcgctgg cgtgggcgtg cgcagaaaat ctggcgaata agattaaggc
    42541 attgacgctg tttgccaccc actatttcga gctgacccag ctaccggaga aaatggaagg
    42601 cgtcgccaac gtacatctcg atgcgctgga gcacggcgac actatcgcct ttatgcacag
    42661 cgtgcaggac ggcgcggcga gcaaaagcta cggtctggcg gttgctgctc tggcgggcgt
    42721 accgaaagag gttattaagc gcgcacggca aaaactgcgt gagctggaaa gcatttcgcc
    42781 gaacgccgcc gcgacgcaag tggatggtac gcagatgtct ttgctgtcag tgccagaaga
    42841 aacttcgcct gcggtcgaag cactggaaaa tctcgacccg gattcactga ctccgcgtca
    42901 ggcgctggag tggatttatc gcttgaagag tctggtgtaa taacaattcc cgatagtctt
    42961 ttgctatcgg gaatattaac gacaactgac gaatcaaata aaaataccct gtataatagg
    43021 aaagcttatt ttacagggta aaaccatgcc atctacacgc tatcaaaaaa tcaatgccca
    43081 tcactatcgc catatatggg tcgttggtga tattcatggt gaatatcagt tattacaatc
    43141 ccgcttacat caactctctt ttttccccga aaccgactta cttatttctg tcggcgataa
    43201 tattgatcgt gggccggaga gtcttgacgt cctgcgcctg ctaaaccagc cctggtttat
    43261 ctccgttaaa ggcaaccacg aagcaatggc gctggatgcg ttcgcgaccg gcgatggcaa
    43321 tatgtggctt gccagcggtg gtgactagtt tttcgattta aatgattgca acaagaggca
    43381 atagatctgt tgctgaaatt ccatcacctt ccacatatta ttgaaatcac taacgacata
    43441 ataaaatatg tcatcgcaca tgcagattat ccgggtgatg aatatctctt tggcaaagaa
    43501 atagcggaga gcgaattact ctggcctgtt tatcgtgtgc agaaatcgct taatggcgag
    43561 ttacaacaaa taaacggcgc tgattatttt atatttggac atatgatgtt tgataacatt
    43621 cagacgttcg ctaaccagat ttatattgat accggatcgc cgaaaagtgg gcggctgtca
    43681 ttttataaaa taaggtagtc tcatgcttct tctgtgaagc atgagtaacc agatgttatt
    43741 gcaggccatt attcattttt cgctaccagc aaagagagat cctgcttcgc cagcgcgcga
    43801 ctggcactct ccggcaaacc gtcgtctgta ataatctgat caaactcgct taatggtaac
    43861 gccagccatg tcgccacctg accatatttc gtcgcatcac agaccaaaac tcgctggcgg
    43921 ctagcactgg caatcgcccg tttcaccgtg actttatctt ccgctggcgt agaaatcccc
    43981 cgcacactcc atgacgatgc agaaataaaa gcctgatcaa tcatcaggct gcgcagcatg
    44041 gtcgcagcgg cttccccgac acaggaacgg ttttcccgac acactgcacc gccagtgtga
    44101 ataattgtgc aattactgtt gtcgagcaag tagttcgcaa taacgaaatc gtttgtgacc
    44161 acagtcagtg actccatgtg aatcagatgc tgtgctatcg ctaacgtggt cgttcccgca
    44221 tccagataga tacaacttcc cggctgaaca agacttgccg ccagcttgcc aatagccgct
    44281 ttttgcgtca ttgccagcgc agtttttacc tgatgagaag gttcatgcgc cacgcgtccc
    44341 ggagactgga cgcctccgga caccagcaca acggctccct gctgctccag tttttgtaaa
    44401 tcccgacgaa tggtcatatg tgacacatta attctgtccg ttagttcagc aatactgaca
    44461 atgccttttt cagctaccat ctcaaggatg atttggcgac gctctacggg tatcaacttt
    44521 tgctccttcc tttgtcctgc tgacattcta cgctatttgc ctgcgaaacg tgcgcggcgc
    44581 aactaacgct tagttcacat aaaataacac aaaatgttaa tttatgtgaa tcagctcacc
    44641 atatcgttat ctcccagcgc ttatattcac aatgtcaaac aaaatatcac ttaaattaac
    44701 aaggagagca gatgaaaacg ggatctgagt ttcatgtcgg tatcgttggc ttagggtcaa
    44761 tgggaatggg agcagcactg tcatgtgtcc gcgcaggttt ttctacctgg ggcgcagacc
    44821 tgaacagcaa tgcctgcgct acgttgaaag aggcaggtgc ttgcggggtt tctgataacg
    44881 ccgcgacgtt tgccgaaaaa ctggacgctc tgctggtgct ggtggtcaat gcggcccagg
    44941 ttaaacaggt gctgtttggt gaaacaggcg ttgcacaaca tctgaaaccc ggtacggcag
    45001 taatggtttc ttccactatc gctagtgctg atgcgcaaga aattgctacc gctctggctg
    45061 gattcgatct ggaaatgctg gatgcgccag tttctggtgg tgcagtaaaa gccgctaacg
    45121 gtgaaatgac tgtcatggcc tccggtagcg atattgcctt tgaacgactg gcacccgtgc
    45181 tggaagccgt tgccggaaaa gtttatcgca taggtgcaga accgggacta ggttcgaccg
    45241 taaaaattat tcaccagttg ttagcgggcg tacatattgc tgccggagcc gaagcgatgg
    45301 cacttgcagc ccgtgcgggg atcccgctgg atgtgatgta tgacgtcgtg accaatgccg
    45361 ccggaaattc ctggatgttc gaaaaccgga tgcgtcatgt ggtggatggc gattacaccc
    45421 cgcattcagc cgtcgatatt tttgttaagg atcttggtct ggttgccgat acagccaaag
    45481 ccctgcactt cccgctgcca ttggcctcaa cagcattgaa tatgttcacc agcgccagta
    45541 acgcgggtta cgggaaagaa gacgatagcg cagttatcaa gattttctct ggcatcactc
    45601 taccgggagc gaaatcatga tcaagattgg cgttatcgcc gatgatttta ccggcgcgac
    45661 ggatatcgcc agttttctgg tggaaaacgg tctaccaacg gtacaaatta acggtgttcc
    45721 aacaggtaaa atgccggaag caatcgacgc actggtgatc agcctgaaaa cgcgctcctg
    45781 tccggtggtt gaagccacac agcaatcgct ggcggctctg agctggttgc aacagcaagg
    45841 ttgcaaacag atctatttca aatacagctc tactttcgac agtacggcga aaggtaatat
    45901 tggcccggtt accgatgcat taatggatgc tctcgacacg ccgtttacgg tcttctctcc
    45961 ggccctgccg gtcaacggac gtacggttta tcaggggtat ttgttcgtaa tgaatcaact
    46021 gctggccgaa tccgggatgc gccatcaccc ggtaaatccc atgaccaaca gctatcttcc
    46081 ccgtctggtt gaagcgcaat ccacagggcg ctgcggcgtc gtttcggcac atgttttcga
    46141 acaaggtgtg gatgccgttc gtcaagagct ggctcgctta cagcaagagg gctaccgcta
    46201 cgcggtgctt gatgcgctga ccgaacacca tctggaaatt cagggagaag ccttgcgcga
    46261 tgccccactg gtaacgggcg gttctggtct ggcgattggc ctggcccggc agtgggcaca
    46321 agaaaacggt aaccaggctc gcgaagcagg gcgtccgctc gctgggcgcg gcgtagtgct
    46381 ctccggttca tgctctcaaa taaccaaccg ctaggtggca cattaccgtc aaattgcacc
    46441 agcccgtgaa gttgatgtgg cacgctgcct ctcaaccgaa actctggccg cttatgcaca
    46501 cgaactggca gagtgggttc tgggccagga aagtgtactt gctccactgg tttttgccac
    46561 cgccagcact gacgcattgg cagcaattca acagcaatac ggtgcacaaa aagccagtca
    46621 ggcagtagaa actctgtttt ctcaactagc ggcgcggtta gcagcggaag gcatgacacg
    46681 ctttattgtc gcaggtggtg agacctccgg cgtagtcaca cagagcctgg gcataaaagg
    46741 gtttcatatt ggcccaacca tttcccccgg cgtgccgtgg gtaaacgcac tggataagcc
    46801 tgtctcactc gcccttaaat ctggcaactt cggtgatgaa gccttttttt cacgagccca
    46861 aagagagttt ttatcatgag cgatttcgca aaagtagagc agtctttgcg agaggagatg
    46921 acgcggattg ccagttcatt ctttcagcgc ggctatgcaa ccggttcggc tggcaatctg
    46981 tcgctgcttt tacctgacgg gaatttactg gcgacaccga caggttcatg cctgggcaat
    47041 ctcgatccgc agcggctttc caaagtcgcc gcggatggcg aatggttaag tggtgacaaa
    47101 ccctcgaaag aggtgctctt tcatctggcg ctgtatcgca acaatccgcg ctgtaaagcg
    47161 gtggtgcatt tgcacagcac atggtcgacg gcgctttcct gcctgcaagg gctggacagc
    47221 agcaacgtta ttcgtccgtt cacaccatac gtggtgatgc ggatgggaaa tgtcccgctg
    47281 gtgccctatt accgaccggg cgataaacgc atcgcacagg atctggcgga actggcagca
    47341 gacaatcagg cttttttact ggcaaatcat ggcccagtgg tttgcggtga aagcctgcaa
    47401 gaagccgcca acaatatgga agagctggag gaaacggcaa agctgatttt tattctcggt
    47461 gaccgcccga tccgttatct gaccgcagg
//
LOCUS       DADMEQ010000018        44100 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.018, whole genome shotgun sequence.
ACCESSION   DADMEQ010000018 DADMEQ010000000
VERSION     DADMEQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.018"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(1296..2978)
                     /gene="glcA"
                     /locus_tag="JNZ05_001066"
     CDS             complement(1296..2978)
                     /gene="glcA"
                     /locus_tag="JNZ05_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HAY8564380.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFEPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
     gene            complement(3333..5504)
                     /gene="glcB"
                     /locus_tag="JNZ05_001067"
     CDS             complement(3333..5504)
                     /gene="glcB"
                     /locus_tag="JNZ05_001067"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HAY8564381.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLCELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMVPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5526..5930)
                     /locus_tag="JNZ05_001068"
     CDS             complement(5526..5930)
                     /locus_tag="JNZ05_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HAY8564382.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(5956..7158)
                     /gene="glcF"
                     /locus_tag="JNZ05_001069"
     CDS             complement(5956..7158)
                     /gene="glcF"
                     /locus_tag="JNZ05_001069"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HAY8564383.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVENVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIV"
     gene            complement(7169..8221)
                     /gene="glcE"
                     /locus_tag="JNZ05_001070"
     CDS             complement(7169..8221)
                     /gene="glcE"
                     /locus_tag="JNZ05_001070"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HAY8564384.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8221..9720)
                     /gene="glcD"
                     /locus_tag="JNZ05_001071"
     CDS             complement(8221..9720)
                     /gene="glcD"
                     /locus_tag="JNZ05_001071"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HAY8564385.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKESLDINPVGRRARVQPGVCNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNYILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            9966..10736
                     /gene="glcC"
                     /locus_tag="JNZ05_001072"
     CDS             9966..10736
                     /gene="glcC"
                     /locus_tag="JNZ05_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HAY8564386.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10956..12002)
                     /locus_tag="JNZ05_001073"
     CDS             complement(10956..12002)
                     /locus_tag="JNZ05_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY8564387.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQDFAALFGAAEAWLKSQGASKICGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKRMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLIGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEM"
     gene            12406..14094
                     /locus_tag="JNZ05_001074"
                     /pseudo
     CDS             12406..14094
                     /locus_tag="JNZ05_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
     gene            14109..15005
                     /locus_tag="JNZ05_001075"
     CDS             14109..15005
                     /locus_tag="JNZ05_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAY8564388.1"
                     /translation="MTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNLTWVRGS
                     LEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGFCQRFLF
                     ISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKPLFDWML
                     RGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDWQRVRQL
                     AAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADWSASNNR
                     ISEDINWFPGISLEHALRNGLF"
     gene            15038..15286
                     /locus_tag="JNZ05_001076"
     CDS             15038..15286
                     /locus_tag="JNZ05_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY8564389.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLNVKPPAQLMETLLPWLENK"
     gene            15286..16458
                     /locus_tag="JNZ05_001077"
     CDS             15286..16458
                     /locus_tag="JNZ05_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY8564390.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTDTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFVVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISPGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(16494..17573)
                     /gene="lptG"
                     /locus_tag="JNZ05_001078"
     CDS             complement(16494..17573)
                     /gene="lptG"
                     /locus_tag="JNZ05_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY8564391.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17570..18640)
                     /gene="lptF"
                     /locus_tag="JNZ05_001079"
     CDS             complement(17570..18640)
                     /gene="lptF"
                     /locus_tag="JNZ05_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY8564392.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18671..19231)
                     /locus_tag="JNZ05_001080"
     CDS             complement(18671..19231)
                     /locus_tag="JNZ05_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY8564393.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(19243..20067)
                     /locus_tag="JNZ05_001081"
     CDS             complement(19243..20067)
                     /locus_tag="JNZ05_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564394.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(20067..21416)
                     /locus_tag="JNZ05_001082"
     CDS             complement(20067..21416)
                     /locus_tag="JNZ05_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAY8564395.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21462..22220)
                     /locus_tag="JNZ05_001083"
     CDS             complement(21462..22220)
                     /locus_tag="JNZ05_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564396.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22252..22965)
                     /locus_tag="JNZ05_001084"
     CDS             complement(22252..22965)
                     /locus_tag="JNZ05_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY8564397.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            23139..23831
                     /locus_tag="JNZ05_001085"
     CDS             23139..23831
                     /locus_tag="JNZ05_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY8564398.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23880..25379)
                     /gene="pitB"
                     /locus_tag="JNZ05_001086"
     CDS             complement(23880..25379)
                     /gene="pitB"
                     /locus_tag="JNZ05_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HAY8564399.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGVNDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25671..27530)
                     /gene="gss"
                     /locus_tag="JNZ05_001087"
     CDS             complement(25671..27530)
                     /gene="gss"
                     /locus_tag="JNZ05_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HAY8564400.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVGHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27735..28601
                     /gene="yghU"
                     /locus_tag="JNZ05_001088"
     CDS             27735..28601
                     /gene="yghU"
                     /locus_tag="JNZ05_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HAY8564401.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGNEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28724..28972)
                     /gene="hybG"
                     /locus_tag="JNZ05_001089"
     CDS             complement(28724..28972)
                     /gene="hybG"
                     /locus_tag="JNZ05_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HAY8564402.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(29106..29447)
                     /gene="hypA"
                     /locus_tag="JNZ05_001090"
     CDS             complement(29106..29447)
                     /gene="hypA"
                     /locus_tag="JNZ05_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY8564403.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29440..29928)
                     /gene="hybE"
                     /locus_tag="JNZ05_001091"
     CDS             complement(29440..29928)
                     /gene="hybE"
                     /locus_tag="JNZ05_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HAY8564404.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29921..30415)
                     /locus_tag="JNZ05_001092"
     CDS             complement(29921..30415)
                     /locus_tag="JNZ05_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HAY8564405.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALELVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30415..32118)
                     /gene="hybC"
                     /locus_tag="JNZ05_001093"
     CDS             complement(30415..32118)
                     /gene="hybC"
                     /locus_tag="JNZ05_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HAY8564406.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVSGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(32115..33293)
                     /gene="hybB"
                     /locus_tag="JNZ05_001094"
     CDS             complement(32115..33293)
                     /gene="hybB"
                     /locus_tag="JNZ05_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HAY8564407.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33283..34269)
                     /gene="hybA"
                     /locus_tag="JNZ05_001095"
     CDS             complement(33283..34269)
                     /gene="hybA"
                     /locus_tag="JNZ05_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HAY8564408.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34272..35390)
                     /gene="hybO"
                     /locus_tag="JNZ05_001096"
     CDS             complement(34272..35390)
                     /gene="hybO"
                     /locus_tag="JNZ05_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HAY8564409.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35579..35866)
                     /locus_tag="JNZ05_001097"
     CDS             complement(35579..35866)
                     /locus_tag="JNZ05_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HAY8564410.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35985..36911)
                     /locus_tag="JNZ05_001098"
     CDS             complement(35985..36911)
                     /locus_tag="JNZ05_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708817.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY8564411.1"
                     /translation="MLCLKKHQLRNATMPRLTAKDFPQELLDYYDYYVHGKISKREFL
                     NLAAKYAVGGMTALALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLV
                     KPAKMSGKTPAVGVVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKG
                     RELQQQVDPTKLMNDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACA
                     VPFYGRQAPTADVAKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVN
                     HGFHNDSTPRYDKSAADLAWQRTLKWFDKYLS"
     gene            37029..38069
                     /gene="gpr"
                     /locus_tag="JNZ05_001099"
     CDS             37029..38069
                     /gene="gpr"
                     /locus_tag="JNZ05_001099"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAY8564412.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKIRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(38109..38603)
                     /locus_tag="JNZ05_001100"
     CDS             complement(38109..38603)
                     /locus_tag="JNZ05_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HAY8564413.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38794..39678
                     /gene="yghA"
                     /locus_tag="JNZ05_001101"
     CDS             38794..39678
                     /gene="yghA"
                     /locus_tag="JNZ05_001101"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY8564414.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     ARLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39950..40375)
                     /gene="exbD"
                     /locus_tag="JNZ05_001102"
     CDS             complement(39950..40375)
                     /gene="exbD"
                     /locus_tag="JNZ05_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HAY8564415.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40382..41116)
                     /locus_tag="JNZ05_001103"
     CDS             complement(40382..41116)
                     /locus_tag="JNZ05_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbB"
                     /protein_id="HAY8564416.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41368..42555
                     /gene="metC"
                     /locus_tag="JNZ05_001104"
     CDS             41368..42555
                     /gene="metC"
                     /locus_tag="JNZ05_001104"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HAY8564417.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVVRVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42695..43354
                     /locus_tag="JNZ05_001105"
     CDS             42695..43354
                     /locus_tag="JNZ05_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8564418.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43394..>44100)
                     /locus_tag="JNZ05_001106"
     CDS             complement(43394..>44100)
                     /locus_tag="JNZ05_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8564419.1"
                     /translation="RVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLNVDILQLQ
                     ELLMDIGEDEHFQPSMAASGINSATLSEEILFAAERLLDVMERPLDARILGKQIIREI
                     LYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEANMSVSAFH
                     HNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSREFKRYFG
                     VTPGEDAARMRAMQGN"
BASE COUNT        10941 a        11759 c        10840 g        10560 t
ORIGIN      
        1 cgtaccgttt aaacaaagac aatacgtctc tttatttctc ataaaacccc aatcccttac
       61 agggcttatt gaaaaattta aaaatcacaa atttctcaat actcgaaatg agatcacata
      121 agaaatcaaa aagccttctc atctatttta agttttccca acggaaaata agagctttca
      181 ttaatactga accactttac tgttctattt gttcaggtat tgcattacgg tgtgctgaga
      241 tccatcggtg catccctgta tcccgcgatg agactaaaat agtcgtgatg taagcttgta
      301 tcagattctt agtgaatacc acatttgtta taattaagtg aaaagataca ttgctgattt
      361 acattatttg gtttacaaaa tgtcatcact tgcagagtta tcgctgacag atacctttaa
      421 tctcttacta ctttcgtgtt attgcaatac aaccatccgt aaagcagggc tatttttagc
      481 aggaaatact caggtgtcaa cattcgaact ccaactctga aatattcata taaattaatg
      541 attttttcat ttcttcgcgc ctaataaaag tattcaaatt cagatgattt ttctgctcaa
      601 cagacacaaa caggcaataa tactctccaa agcgaacaat atttggcatt acgatttaat
      661 gcttcataat caccaaatta tttaaccata aattttctta tttctaacaa aatgattggt
      721 gtgttgttgg agttaaaata catacataat attaacatct gccaacattt cactttccgc
      781 aagacggatg atttcgaatc ctcatccacg ttaccctatc ccaaatatct ttattaatag
      841 gcagtaacga acactgttat agcaagatta acttattaac ctaacggcat cttttatgta
      901 acaagtggag ataaattgct taaaattgat cctcgacaaa tcttaaaagt tctcattatt
      961 ctgcaaaatc agctttgcga ataatcttat gtttttggga gttttgggaa ttttgatcta
     1021 ctcattgttc tagtgaaaaa tataaataag agaaaaaacg ccattcgtca tgaaagcaac
     1081 agatgaagca atgtaataac atactaaaat tgatctcatg aaagtgggta ctttataacc
     1141 agcacagaag ccaagttgta taaatattaa acaattcgca tcgtgaaacg gagactacgt
     1201 ataaagaagc caatcgtaaa aacggttccc tggcgagata aacgacagac aaatggaaag
     1261 ccgggtgata tttaccttca tccggcttta ttgatttacg agactaacat cccggtaaac
     1321 acatacgcct gcagcagggt gataacgccg ataacgctgg caaaaatcag actgtgcttc
     1381 acggtgtagc ggaacagttc agattctcgg cccaccatgc ccgtcgcggc gcaggccacg
     1441 gcgatagatt gcggggagat catcttgcca gttacgccgc cgctggtatt tgctgccacc
     1501 agcagagtgt cagagacgtt gatttgctgc gccgtggtcg attgcagtga accaaacagg
     1561 gcgtttgagg aagtgtccga cccggtaagg aatacgccca gccagccgag aaacggtgag
     1621 aaaaacggga acatcacgcc tgtacctgcc agtaccagcg ccagcgtggt ggacatgccg
     1681 gaatagttgg tgacgaaggc gaatgccagc accatgccaa tcgacaatat cggccacttc
     1741 agactaatta gcgtttcggc aaagacgcca atccctttct tgatgcccac gccgaggatg
     1801 aagatagaga taatcgccgc aataaaaata gcggtgccgc cagccgagag aggttcgaat
     1861 ttaaacaccg catccattgg cgttggttgg gcgacaatgg gtgccgcttt caacacttgt
     1921 tgatgcaaat gagggatctg gaaattaatc accagtgaat aaaacgcgcc gcccggagca
     1981 aataacgctt taaacggctt catggtccag atggtgacca gcaccgttaa gattaaaaac
     2041 ggtgaccacg cacgaatgat ttgccccaga ctatattctg aaggcacggg accgccagaa
     2101 gatggcttat ttaccaccat cgcacctgcg gattgcccca tgctgattgc cgtttcggta
     2161 tttttcggcc gccagacttt aaggaataaa gcgagtgaga cgatactcac cagcgccgaa
     2221 gtaatatccg gcagttccgg accaatatag ttagaggtaa agaactgagt gacagcgaag
     2281 cttcccccag caaccagcgc cgctggccac gtctctttca cccctttcca gccgtccatc
     2341 attgctacca gccagaacgg cacaagaacc gacaggaacg gtaactgacg tcccgccatt
     2401 gcgccaatgt ggaacggatc gattcccgtt acctgaccgg cgaccagaat cggcacgccc
     2461 aacgcaccaa acgccaccgg cgcagtattg gcaatcagac acagccccgc cgcgtataac
     2521 ggtttgaagc ccaggcccac cagcagcgca ccggtaatcg ccaccggcgc accaaagcca
     2581 gccgctcctt ccagcaacgc accaaaggag aaaccaatca gtaacacctg caaacgctga
     2641 tcgtcggtga tggagataac cgagctgcgg ataatatcga actgcccgct ggcaacggtt
     2701 aatttataca ggaacaccgc cgcgacaata atccacgcaa ttggccataa tccataaata
     2761 aagccatagc ccgcagcagc aaatgccata tcaatcggca ttttaaaggc gaatatcgca
     2821 atcaggatag ataatataag ggttattgct ccagcgacat gtcctttcag acgtaatacc
     2881 gcgagtgcaa cgaagaagaa tattatcggg atcagggcga ccagagcgga tagccccagt
     2941 cctcccatcg gcatatacat ttgggtccag gtaaccatat tgttcagtct cttattatct
     3001 ttatatgctt gatatactta aggttgtaat aagcaaaaga ggactgaact gtaaaatata
     3061 ggcgttatac tttacagcaa cagtacgccg ctaacgcaat tgctacctct ggcataacaa
     3121 gtatatcggg taagggtttc tgttccgcac acgcaggcgc agagtatcgt taagatgtcc
     3181 atattgttgt tttaggcccg ctagtaatgc gctacgggta tttaatattg ttaaaccctg
     3241 ataatcgctc cggttatttc cgggataaat gtactaccgc agttactatc atagccccga
     3301 caataaaact tgccggggct tttttgacgc tattaatgac tttctttttc gcgtaaacgc
     3361 caggcgtgta ataacggttc ggtataaccg tttggctgtt tcacgccgag gaagattaaa
     3421 tcgctggcag ctttaaaagc acacgagtta gcgaaattcc ccgccatcgg acgataagcc
     3481 ggatcgccag cattttgctg atcaaccact ttcgccatat tctccagcga cgcctgcacc
     3541 tgttctttgg tcagaatacc gtgacgtaac cagttggcga tatgctggct ggagatacgc
     3601 agcgttgcgc ggtcttccat cagcgccacg ttgtgaatat ccggcacttt tgaacaacca
     3661 atcccctgct ccacccaacg caccacgtac cccagaatcc cctgcacgtt gttatccagc
     3721 tcttgttgga tctcttgcgc cgaccagtta gcgttttcag ccaccggaat agtcagcaga
     3781 tcgtccagca gcggttcaaa ttcagcattg aactcggtct gggcaatgtt ggcttgcacg
     3841 ctctgtacgt tggtttggtg gtagtgcagc gcatggagcg tagcagcggt tggtgacgga
     3901 acccaggctg tgtttgcccc ggcacgcagt tggtcgccct tctggctgta catgtctgcc
     3961 atcaggtccg gcattgccca catgccttta ccgatttgcg ctttaccgcg cagcccacag
     4021 aacagaccgg aaagcacgtt attacgttcg taggctttga tccacggcgt cgatttcatc
     4081 tggtttttac gcagcatcgg gccagcttcc atcaccgaat gcatttcatc gccggtacgg
     4141 tcgaggaaac cggtattgat gaacgccacg cggttgcgcg cctgagcgat acagctccgc
     4201 aagttcagcg aggtccggcg ttcttcatcc ataatgccca ttttcagggt attcggtacc
     4261 ataccgagca ttgtctcaat gcgggtaaac agtttgttgg cgaacgccac ttcctgcgga
     4321 ccgtgcattt tcggtttcac aatatagacg ctgccagtgc gcgagttttt ctgcactttt
     4381 aaatcataga gggcaatcgc gccagtcatg acgccatcaa gaataccttc cgggatttca
     4441 ttgccttcgc tgtcccaaat cacaggaatg gtcatcaaat gacccacgtt gcggataaac
     4501 agcaacgagc gtccgtgcag agaaatttca gagccatcgg cggcggtgta ctgacgatcg
     4561 tcattcagtt tacgtacgat ttgccgaccg tttttctcca ttttctcttg cagagtcccc
     4621 tgcatcaggc ccagcagatt gcggtacagc aggattttat cttccgcatc aaccgccgcg
     4681 accgaatctt cgcagtcgag aatggtactg atagcagctt cgacgataac atcgttgatg
     4741 tgcgccggat cgtctttgcc aatccgcccg ttggcatcaa tttgcagttc aatatgcagg
     4801 ccgttatttt tcagcaaaat gcaggtcggc gcagcggcat cgccacggta accgacaaac
     4861 tgtgctggag tacgtaacgt ggtttcttta ccatttttca actggatgcg taattgttta
     4921 tcgaccacct taaacgccac cacatcctga tagctgccgt tttccagcgg tagtgattca
     4981 tcgaggaaac gtcgaaccca ggcgataacc tgctcaccgc gctgcggatc gtagccactg
     5041 accatcgccc cttcctgcgg gatgatgtcg ctgccgtata acgcatcgta cagtgagccc
     5101 cagcgagcgt tcgccgcgtt cagcgcgtag cgggcgttca ttgccggaac caccagctgt
     5161 ggccccgcct gactggtgat ttcgctgtca atgcccgtgg tttccaccgt cacgcgctcc
     5221 ggttgcggca ccaggtagcc cagttcacaa aggaaagatt tataggccgc tttatctttt
     5281 accggccccg gattgctgcg atgccactca tcaagcgccg cctgaatgcg atcgcgttct
     5341 gccagcaact gacgattttc tggtgccaga tcgtgaacga tctcatcaac attgtgccag
     5401 aatgcagcag cgtccagttc cacgcccggc aaaacttctc catccacaaa acgtttaaaa
     5461 ttggcgtcaa tgcgtaaacg gccctgggtt atggtttgac tcattgtttc tctcctcgtt
     5521 ttcgcttatt tcgccaacac cgctgctgcc gctttcgcga cctgcgcatc ctgtgctccg
     5581 gttaaaccag aaacgcccac ggcaccaata atttgcccat caacaacaac cggtacgccg
     5641 ccttccagcg acgtcagtaa cggcgcagtc acgaacgcgg tacgtccgtt gttcaccatc
     5701 tcttcatagc ccttagtttc acgacgcccc agcgcggcgg tacgcgcttt ctcctgggag
     5761 atgtaagccg caatcggcgc gcaatcgtcc atgcgactta acgccagcag atgaccgccg
     5821 tcatcggcaa cagcaatgga aacagaccag ttatttttct gcgcctcttc ctgacctgcg
     5881 gcaataattg cactcgccat ttgctggcta agaatgactt tagttttcat ttttttattc
     5941 cttttcaagg gcttgttata caatttcaat ccagtgacgc acagaggtac gaccggcgct
     6001 cgccagatgc gtctggcaac caatgttggc ggtgacgatc atttccggtt tgccgctttc
     6061 cagcgcattc attttgttat cccgcagctg gcgtgccaga tcgggatgcg ttaacgcata
     6121 tgttcccgct gaaccgcagc acagatggct gtcgggaacg tccgttaagg taaatccaag
     6181 acgaagtaac acgttttcca cttcgccgtt cagcttttgc gcatgttgta gggtacacgg
     6241 gcagtggaag gccagttttt tatcgccgcg aattgccagt ttttccagcg gttcctcgcg
     6301 cagaagttcg actaaatcga ccgccagttc actgacctga cgcgctttat cggcatataa
     6361 cgcatcgttt ttcagcatct gcccatactc tttgacgaac gcgccgcaac cgctggcggt
     6421 ttgcaaaatt gcctcggcac ctgcttcaat cgcgggccac caggcatcaa tattattgcg
     6481 cgcccgtgcc aaccctttct cctgcgcatt aagatgatag tccaccgcgc cacaacagcc
     6541 tgcttcgtta gctgacatga cgctgatccc cagacgatcc agcactcgcg cagttgccgc
     6601 gttggtgttg ggcgaaagcg taggctgggc gcagccttcc agcattaaaa cccgacgctt
     6661 atggcgcagc ggcggacgcg gtttagcttt caccgtttca gcaggcagtt ttgctctgac
     6721 ctgttccggt aaaaacggtc gcagcaccag ccctacctgc gtcagcgcac ggaagaccgc
     6781 cggacgcggc actacctggc gcaatccttc gcgcagcatt cgctccggca gtgggcgttt
     6841 cactttctgc tcgacaatat cacgcccgat atccagcaaa ttgtgatagc gcacaccaga
     6901 aggacaggtg gtttcacaat tgcggcaagt gaggcagcga tcgagatgct cctgtgtttt
     6961 aagcgtgact tcgttgcctt ccagcacctg tttaatcaga tagatgcgcc cgcgcggccc
     7021 gtccagttca tcgcccagaa gctgataggt tgggcaggtt gcggtacaaa atccgcagtg
     7081 aacacaggcg cgcaggatgc tgtcggcttc cagcgcgcgc gcgttctgcc gcatctcttc
     7141 ggttaatcgg gtttgcatag cctgctcctc aaagttccgc gtacatgcga ccggggttaa
     7201 acacgccgca agggtcgagc tgctgtttaa gctgctggtg atagcggaat aaaggagccg
     7261 atagcggggc aaagccacca tctccggcac taaagcgggt cgcatgaccg ccagcgttgc
     7321 gggcgatgcg atggatttga ttgtcatcgg ctgtcgattt cagccagcgt aacgccccgc
     7381 cccagtcgat cagttgctcg ccgggtaaat ccatcatcgg cgcatcgctg ggtaatgaaa
     7441 tgcgccataa ggtacctggt aacgagaaga acggcagttg ttgttcacgc aattgctgcc
     7501 agaactgacc cgcaacctct tcgccaccca gcagttcacg cgctgctttt accgatcctt
     7561 cgccgccctc aaggcggatc cacaacgcat tgtcgaagta acataagcca ctaatgggta
     7621 atggctggag ttgccactcg gcgatttcac tcatggcttc ttgcaggctg atttcccgac
     7681 gcaggctcag agaggcacgc ggtcgcggta acactttcat tgagatttca gtgagcacgc
     7741 caagacaacc gtagcttccg gccattaacc gtgagagatc gtatccggca acgtttttca
     7801 tcacttcgcc accaaaacgc agatgttttc cagtgccggt aatgatgcgc gtgccgagga
     7861 caaaatcgcg gaccgaaccg ctccacgggc gacgcggccc cgccagcccg caggcgacca
     7921 tcccgcccca ggtggcttct tcaccataat gcggcggctc acagggaagc atttgcccgg
     7981 cgctctccag cgccgcttca atcgccacca gcggcgttcc ggcacgagcg gttatcacca
     8041 gctcggtcgg gtcgtaatta acaatgccgc gatgacaacg cacatccagc gtttgcccgg
     8101 tgactgggcg acctaaaaag gctttgctat tgctgccctg aatcaccagc ggcgttttat
     8161 cgctaattgc ctgattcacc tgctccagca gcgcctgact gtaatcacac tcgcgtagca
     8221 tcaaaagcgc tccagttcag gaaaaggtaa atgaccgtga tgcacatgca tggcaccaaa
     8281 ttcagcacag cggtgtagcg tgggaatgtt tttcccaggg ttcagcaaac catcggggtc
     8341 aaacgccgcc ttgaccgcat ggaaggtcgt gatttcatcg ctgttgaact gggcgcacat
     8401 ttgattgatt ttttctcgcc cgatgccatg ttccccactg atgctgccgc caacttcaac
     8461 gcagagttcg aggatcttcc cgcccagctc ttccgcgcgg gcaaattcac caggttcgtt
     8521 ggcatcgaaa aggattaacg ggtgcatgtt gccatctccg gcatgaaaga cgttggcaac
     8581 acgtaaatca tattgctgcg ataaacgggc aatgccttcc agtacgccag gcagggcgcg
     8641 acgcgggatg gtgccatcca tgcagtagta atccggggag atacgtccta ccgccgggaa
     8701 cgcatttttg cgaccggccc agaaacgtac gcgctctgct tcgtcctgtg ccaaacggac
     8761 gtcagtcgcg cccgctttca acaagatgta gttaacccgc tcgcagtctt cctgtacgtc
     8821 agactccacg ccgtccagtt cgcataacaa aatcgcttcg gcgtcgacgg gataaccggc
     8881 atgaataaaa tcttccgccg cgcggatcga caggttatcc atcatctcca gcccgccggg
     8941 gataatgcca ttggcgatga tgtcaccaac cgcaagtccg gctttttcta ccgagtcaaa
     9001 gctggctaac agaacccgcg ccacgggcgg cttcggcagc agttttaccg tcacttcggt
     9061 ggtcacgccg agcatacctt ccgatccggt gaacagcgcc agcaggtcaa aaccaggtga
     9121 atccagcgcg tccgatccca gcgtcagcgc ctcgccgtcc agcgtttgca cttcaatttt
     9181 cagcaggtta tgtacggtca gaccatattt caggcagtgg acgccaccgg cattttccgc
     9241 cacattgccg ccaattgaac aggcgatttg tgaggaagga tccggcgcgt aatagagatt
     9301 atgcggcgca acggcctggg agatcgccag gttacatacg cccggctgca cgcgcgcgcg
     9361 gcgaccaacg gggttaatgt cgaggctctc tttaaagcgc gccatcacca acaacacacc
     9421 tttttccagc ggcagcgcgc caccagaaag cccggtgcct gcaccacggg tcaccaccgg
     9481 tacacgcagg cgatggcaga cagccagaat cgctgtcacc tgttccattt gcttaggcag
     9541 aacaaccagt aatggacgcg tgcgatacgc gctcaacccg tcacactcgt aaggaatgat
     9601 ctcctcatcg gtatgcagga tctcaagtcc agggacatgc tcacgcagtg ccatcagtac
     9661 cgatgtgcgg tcgacatcgg gtaaagcgcc atcaagacgc tcttcgtaca agatgctcat
     9721 gagtaggctt cgctttgttg tgtggcagct gatttttgcg cgctgcttct gtgaacagtt
     9781 attaagcggg cttttcgttt tcgtctatct ctttagctac cggtcagacc attttttttc
     9841 cagctctgtg accttgtctt ggttaactta atgttaaatt gatgtaacat aatcacttgc
     9901 gtgatgtgcg tgttttgcga gttaagaaca gaaaaattgg tcctacctgt gcacgaggtc
     9961 cgggaatgaa agatgaacgt cgccctattt gcgaagtggt tgcagagagt atcgaacggt
    10021 taattatcga cggcgtactg aaggtcggtc agccgcttcc ctcggaacgt cgactgtgtg
    10081 aaaagctcgg cttctcacgc tccgcactgc gtgaagggct gaccgtgctg cgcgggcgcg
    10141 ggattattga aacggcgcag ggtcgcgatt ctcatgtcgc acggcttaat cgggtgcagg
    10201 acaccagccc gctgatccat ctgttcagta cgcagccgcg aacgctgtac gatctgctcg
    10261 acgttcgcgc attactggag ggcgaatcgg caaggctggc ggcaacgctg ggaacgcagg
    10321 ctgattttgt tgtgataacc cgctgttatg aaaaaatgct cgccgccagt gagaaccaca
    10381 aagagatttc gctgatcgaa catgcgcagt tggatcacgc tttccatctc gccatttgtc
    10441 aggcttctca caatcaggtg ctggtgttta cgctgcaatc attgaccgat ctgatgttta
    10501 attcagtgtt tgccagcgta aataatctct accatcgacc acagcaaaaa aagcagatcg
    10561 atcgccagca tgcgcggatc tacaacgcgg tgttgcagcg gctgccgcac gtcgcgcagc
    10621 gcgcagcgcg cgatcatgta cggaccgtga aaaagaatct ccacgatatc gagctggaag
    10681 gccaccattt gattcgctcg gcggtgccgc tggagatgaa caaggtggga atgtgagcag
    10741 agttttaccc tatccccaag gcgaggggac cgcccctgca cttaacagga tctggtgtta
    10801 aatctgttca tctataccct ctcccgaatg accgcccctg cgctaaacag gatctggtat
    10861 taaatttgct tttcatacaa tcggtaacgc ttgtacggct ccgccccaat gcgttccagc
    10921 atgttattca tgcctgtatt ggtttcgagg atccattaca tctccagcgc atcgatcttc
    10981 cggcgggcaa acggatcgcg taaggcttca atcaataaca gcgcaatcac cgggccgatg
    11041 cggctgaact gatactcgtc gcgcacgccg atcagcggta ctcgcgcagt tcgcacaccg
    11101 ctgactttca aacgccacag caattttgcc cagccgaagg gaaagagcga tccgttcaga
    11161 tcggcaatcg cctcgttgat gttaggcaag ccgacaataa acgcgcaggg tgcagaatca
    11221 atctcagcga tgtagatcat atcgtccggc accagatatt taagttggtc gcccatggtc
    11281 gcgaattcat gttcggtaaa cggcacaaat ccccagttgt gctgccagcc agagttgaaa
    11341 atctcacgca ggatctgcat ctcttcggca aaccgctgac gattgatgca gcgaatggtc
    11401 acctttttgc gcacctgatc catccgtttt tttagcgccg gagagaaagt gagatcggtt
    11461 cgctgcatcc accacgccag taaatcaatg cctttgtgat aacccagttg ttcaatatgc
    11521 gcggcatacc acggtttgcc gtgtggcatc atcgcacagg gtggtgtgtc aaaaccttca
    11581 atcagtaatc cgctttcctg attgatattc aggctgaaag gcccgcagat cttacttgca
    11641 ccttgtgact tcaaccacgc ttccgctgcg ccaaacaacg cggcaaaatc ctgcggatca
    11701 tcaatggcgt caatcatgcc gaaatgaccg gtatcttcac cgtaacgctc acggtgcaag
    11761 gtatctattt gcgcggtaat acgcccaact atctgccccg ctttttttgc cacccacgcc
    11821 tgccagatga tatggtccgt ccccggattt ttcgcagaca aatgctcgtt gcgttcaatg
    11881 aataaagggg gtatccagtt tggatcgtcg ggataaagtg atgacggaaa agcgataaat
    11941 gccttaaggt catttttatt aattactttt tcaattttaa tcatcagcag atcgcattcc
    12001 atatctctgt ggataacaaa taattaatgg tgaaaaacag gttgcaacag caacgagcgg
    12061 catcttaata atcaactcaa tgaatacaaa cgacgaataa atagtattaa ttaatgatta
    12121 tgataaggtc agaggctaca cagaaacgcc tcaatgcctt ttgtcgattt aaggcgaccg
    12181 gacaaggctt cttcttaata tattcataaa ttaaatatca ttcataataa aaataaaaaa
    12241 ctcaaactca ttatttaaat caatgttcat tttcacaccg atgaaaatac tatctcattt
    12301 atcttttatg cctatataat ccaccgcatt attgtcattt tatttttgat atccaatttt
    12361 ttcgtggtgt atatgtctaa taaaatcttt acgcattccc tacctatgcg ctatgccgat
    12421 tttccaacgc tggttgatgc tttggactac gccgctctga gtagagccgg aatgaatttt
    12481 tatgacagac gttgccaact tgaagatcaa ctggaatatc agacattaaa aacgcgtgcc
    12541 gaagctggtg cgaagcggtt gttatcgctg aacctgaaaa aaggcgaacg cgtggcgctg
    12601 attgccgaaa cgagtagcgg gtttgtagag gctttttttg cctgtcagta tgctggctta
    12661 gtcgccttcc cgttggcgat tccaatgggc gtcggtcagc gggattcctg gagcgccaaa
    12721 ctccagggtt tactggcaag ttgccagccc gcagccatta tcactggtga tgagtagttg
    12781 ccactggtca atgccgcgac gcataacaac cccgaattac atgttttaag ccacgcttgg
    12841 tttaaggcat taccggaagc cgatgttgcg ctccagcgtc cagttccgaa cgatatcgcc
    12901 tacctccagt acacctccgg cagcacccgt tttccccgtg gcgtcattat cacccatcgc
    12961 gaagtgatgg ctaatctacg tgctataagc cacgatggca ttaaattacg ccctagcgac
    13021 cgctgcgtct cctggcagcc tttctaccat gatatgggac tggtcggctt tctcctgccc
    13081 cccgtcgcca cgcaactttc agtagattat ttgcgcactc aggattttgc catgcgtcct
    13141 ttgcaatggc ttaaattgat cagtaaaaat cgcggcaccg tttccgttgc gccgccgttt
    13201 ggctatgaat tgtgccaacg ccgcgtgaat gaaaaagatc tcgctgaact ggatctttcc
    13261 tactggcgcg tcgctggtat tggtgctgag ccgatctccg cagaacaact ccatcaattc
    13321 gctgaatgtt tccgtcaggt taactttgac aataaaactt tcatgccgtg ctacggactg
    13381 gcagaaaatg cgctggctgt cagcttctct gatgaagcct ccggggttgt ggttaacgaa
    13441 gtggatcgcg acatcctcga atatcagggt aaagccgtcg cgccgggtgc agagacacgc
    13501 gccgtatcga ctttcgtcaa ctgcggcaaa gcgttgccgg aacatggtat tgaaatccgc
    13561 aatgaagcag gtatgccggt cgcggaacgt gtggtaggcc atatttgcat ctccggtccc
    13621 agtctgatga gcggttactt tggcgaccag gtttcgcaag acgagattgc cgcgacgggc
    13681 tggttagaca ccggcgacct cggttatctg ctggacggtt atctgtatgt caccggacgc
    13741 attaaagatc tgattattat tcgtggccgt aatatctggc cgcaggatat tgaatatata
    13801 gcggaacagg aaccggaaat tcattctggc gatgcgattg cttttgttac cgcccaggaa
    13861 aaaatcattt tgcagatcca gtgtcggatc agcgacgaag aacgtcgcgg gcagcttatc
    13921 cacgcgctgg cggcacggat ccaaagcgaa tttggcgtga ccgcggctat cgatctgttg
    13981 ccgccccaca gtattccccg aacgtcctcc ggcaagcctg cccgtgcgga agcgaaaaaa
    14041 cgttatcaga aggcttatgc tgccagtctt aatgtgcagg aatccctggc atgaatcaaa
    14101 ctgtcgcggt gacgggcgct accgggttta tcggtaaata tattatcgat aacctgctcg
    14161 cccgcggctt tcacgttcgc gcattgacgc gtactgcccg cgctcacgtc aatgataatc
    14221 tcacctgggt gcgcggttcg ctggaagata ctcattcact tagcgagctg gttgccggag
    14281 ccagcgtagt ggtccattgc gccgggcaag tgcgcgggca caaagaagag attttcaccc
    14341 gctgtaacgt tgacggcagc ctgcgcctga tgcaagcagc aaaagagagc ggcttttgcc
    14401 aacgttttct gtttatctct tcgctggcgg cgcgccatcc cgagctctcc tggtatgcaa
    14461 aatccaaata cgtcgccgag caacgactgg cagcaatggc ggacgaaatt acgctgggcg
    14521 tttttcgccc gacagccgtg tatggtcccg gcgataaaga gttaaaaccg ctgtttgact
    14581 ggatgctacg cggcctgctg ccacgacttg gtgcgccaga tacacaactc tcttttcttc
    14641 acgtcaccga ttttgcgcaa gcagtgggtc agtggttaag cgccgaaact gtacagacgc
    14701 aaacctatga attatgcgat ggcgtcgccg gtggctatga ctggcaacgc gtacgacagc
    14761 ttgccgccaa agcccgttgt ggttccgtgc gaatggttga cattcctctg ccggtactca
    14821 caggccttgc ggatatcagt accgcgttga gtcgcctggc aggtaaagaa cctatgctga
    14881 cccgctcgaa aattcgtgaa ttaacccacg ccgactggtc ggcaagtaat aaccgtattt
    14941 ctgaagatat caattggttt cccgggatta gcctggaaca cgcattacgc aacgggctat
    15001 tttgattgtc ttgttttaaa ggtgtgttaa cgtttctatg gtaaatcgtg aaatagtaat
    15061 ggattacatc ctttcctgtt tacaggattt ggtcgaaaac ggtgtggaaa ttaagcccga
    15121 cagcgatctc gtcaacgatc ttggcctgga gtcgatcaaa gtgatggatc tcttaatgat
    15181 gctggaagac agattcgata tttccattcc tattaatatt ctgctgaatg tcaaaccccc
    15241 tgcgcaatta atggaaactc tacttccatg gctggagaat aaataatggg gctatacgat
    15301 aaatatgcgc gccttgctgg cgagcgtctg caatttagtg acaacgggtt aacaccgttt
    15361 ggcacctgta ttgatgaagt gtattccgcc accgaaggcc ggatcggcaa taaaaaagtg
    15421 attctggccg ggaccaataa ttatctcggt ttaaccttta atcatgatgc catcagcgaa
    15481 ggtcaggccg cgttagcggc ccaaggcacc gacaccaccg gttctcgtat ggcaaacggc
    15541 agttatgcgc cgcacctggc gttagagaaa gagattgccg agtttttcaa tcgccctacc
    15601 gctatcgttt tttcaaccgg ttataccgct aatttaggcg tcatcagcgc acttgccgac
    15661 cataacgccg tggtgttact ggatgccgac agccacgcca gtatttatga cgcctgttct
    15721 ctcggtggcg cggaaattat tcgtttccgc cataacgatg ctaaagatct ggaacggcgc
    15781 atggttcgcc tcggtgaacg ggcgaaagag gcgattatca tcgttgaggg catctacagc
    15841 atgctcggtg acgttgcgcc gctggcggaa attgttgata tcaaacgtcg tctcggtggt
    15901 tatttgattg tcgatgaagc gcattcgttt gtcgtgttag gcgcaacggg gcgcggactg
    15961 gctgaagctg tcggcgttga agatgatgtg gatattatcg ttggtacttt cagtaaaagt
    16021 ctggcctcga ttggcggttt tgctgtcggt tcggaagcga tggaagtgct gcgctacggc
    16081 agccgcccgt atatttttac cgcctctcct tctccgtcct gcattgcgac cgtgcgctcg
    16141 tcgttaagaa ctattgccac acaaccagag ctgcggcaaa agctgatgga taacgccaac
    16201 catctgtatg acggtctgca aaaactgggt tatgagttaa gttcacatat tagcccggta
    16261 gtgccagtaa taatcggttc gaaagaggaa ggtttacgca tctggcgcga gcttatctcg
    16321 ccgggcgtgt atgtgaatct tatcctgccg cctgccgcgc ctgcaggcat cacgctgctg
    16381 cgctgtagcg taaatgctgc ccacagtcgt gagcaaatcg atgccatcat tcaggcattt
    16441 gctactctga agcagtaatg tgagcccgcc gtggtgtttg cgccacggcg ggtttaatgt
    16501 tgtctgtcat accaatacac caggctcaac gccaccaaca caaacgctac cggtaaaccg
    16561 agcgcggtaa caggtgcgct taacgcaaat agcaatccca gattaaccat gatttgatag
    16621 ctgatccagg tgagtaagcc cacgatgacg cctacagcga gacggctacc catcccaggc
    16681 gaacgcgggg cactaaaggt aaacggcaca gccagcaaaa tcatcgccag ggtcaggatg
    16741 ggttgcccca gtttttccca cagagcaagg cggtattcgc tgctgggttg cccggtttcc
    16801 tgcaaataat ggatgtaatg attaagctga cggacagaaa aagtattccc cggcattgat
    16861 aactcttcaa gattcatact ggtgaaggcc gattgccagg caagattatc taatgtttcc
    16921 agcgtttctt tgccattcag ccatttttta tgattcacac catgcaggac ccacgttttg
    16981 tcgtcttcaa tggtggcact gcgtgcataa atgtaggatt caagcgagag atcgtcgcga
    17041 taatgaaata tctccacgcc caccggctgg ccctgctcgt tcagcgattt cactgtcaca
    17101 aattcattac cacgcctggc ccacagcata ttgccggtaa tgtcatcgtc ctcccccaac
    17161 gcggtggcgg tcgatttgat ttgcaacgcc tgttgctgca atggcgacgc cacccactca
    17221 tctatcgcgc ctaacgaaac agtccacaat atcccggcaa ccagcgccac cagtgcaata
    17281 cggaagatag aaaaccccgt gctgcgaatg gcggtaagct cactgttttt cgataactgc
    17341 cccagtccaa caataccacc caataacgcg ataaacggcg aaagttcgac cagtgtgcgc
    17401 ggtaaggtca ttagcaccac cagcaccgcc tgagtccagc gataaccgcc cgggctgaca
    17461 ccatccagtt cgttaatcag gttgaaggtg gtaaaaagcg gcagcaatag ccctgcagcg
    17521 gcggcaaaac cgagaaagag atgacggatt aaatagcgac tgaaaacatt catcgggaaa
    17581 atttctgcag caaagagaag tcacgtgcga ccagtatcag caggcccatt agcatgagtc
    17641 cgggcactaa ccacaaacca ggaatgaggg gaatcgcgcc gttagccacc agtgtacggc
    17701 agacgtcgcc gccataaaaa atggcaataa acagcaacgt cagtggcaat aacgttgaaa
    17761 atcgcccttg ccgcggtttt acccgactta atgaaatggc cagcagcgcc atcaacaatg
    17821 cggtcaggcc acggctttgt cgccattgca gttcggcatg atcggcagga aaggcggagc
    17881 gcgccagctc cgtgaccgat ttcgctttgc gtttgacgtt cgggctttga tccagcggat
    17941 tcagatgtaa ttgcagatta cggtagatct gttcgttgtc gtcacgcccc tgatgatcga
    18001 gaagataggc agtgccgttg tgcaacataa cggtcggttt ctcgggtgat gggtcaacca
    18061 catcaaccga acgggcgcgg aaaatacggg ttcgattggc agtagaagta taaatcagcg
    18121 cgtcagtcag gcgattatta tcctgatcaa ccgtctgcga aaggatcatt cgtccgttat
    18181 cgttagtgtt aaatttcttt gcccgcaact ggcgaacatc cagctccgac tgcgactgct
    18241 gctccagttg ataaatctgc gcataagccc acggacgacc atacatcgac agaagggtga
    18301 caaaaatact tagcggtatc gccagataaa gaacggcttt gtacaagcgt cccggactgc
    18361 cccccgccgc agagatagcg gtaatttccg agtcggtata catctgccct agcgtcacgc
    18421 caactgacac atacagacca acaggtaaca acatctccag tgcaatcagt actttgtaga
    18481 aaacgatatc cagcacaaca tcaagagcta atgtcccatt tgccgcttcg gtcagataac
    18541 gctgtgcgga gtagctggcg aaaataaaga tcagaaaacc gacgataatc agcaccagac
    18601 ggcgcgtacc acgcatgatg tagtgttcaa ccagcttcat ctcactaacg ccctcaatcc
    18661 tttgagtttt ttattcattg atcagtttct gcgccaacgc aatgacatga tctacctctt
    18721 ccggcgtcag gctgccgtcc tttgcccact gaatttgccc ggctttattc agcaccagaa
    18781 tggttgaact ctgctccggc aagcgccagg caacgcgtcc ctgtccattg ccgtcaataa
    18841 cgaattgcgc ccacggatag cggcgtttat ttttttcgat tttgccaaca acaaaatagc
    18901 cagtaccaaa gatggcatca tcggtgttca caatggtggt gggctgaaaa cgatcctgcg
    18961 gaaagtttgc cgcttcaacc gctttaatta acagtgagtt ctttttcttc gccgacttac
    19021 gcccggcgat atactgaata atgcggactt ttcccgctaa ttgtgtgctg ttccactttt
    19081 ggtagtgaat ttcgtcgtcg ctattcagaa gcagctcgcc gccttcctgg atataaacag
    19141 gggtaaccgt tttgccagtg acgaaattgt gtgcctgcac acccggggct aagagcatga
    19201 taatcaacgc aataatcaaa cgagatgaca tcattgctcc ggctatagcg gaaaacgcga
    19261 aatcagcagc tctaagcgct gacaaatatc gctatccagc gccaacggtg cataatacga
    19321 ctggcggttt tccatatcat ccgccaccat gtcgtcgtgt ttatctccca tcaggccatt
    19381 aatggcggcg aggaaacggg aaatctgtaa cgcctcttta ttttcaggat caagcgatct
    19441 taaggtgtcg taataagaaa cctgctggct gatgattttt cgcacatcat gaaattccct
    19501 gccggttagt cgcggtttgg caataatctc tttcagggta tgcatttgct tttgccgata
    19561 ggcaataaaa ctgcttacgc tttctaactg gctatggcgt aatgaatagt tcaccaacga
    19621 ccagaccgga gagcttaaat aaatccgtaa caggttgccg taatacgaca caatattttt
    19681 cttaccgtta cgaaagccgt cctgaaaacg ccccagaaaa acggtcgttt tgccgaataa
    19741 aaatccggcc tgatgcttct ttaaatacag ccgttgcgca aaacgcaggt gcttgtagcg
    19801 cgctcgcatg tatttaaatt gtttgcgctc atcagcagtc agctcatcgc ctttggcttg
    19861 tttgcgaact aaacgcagta attcctgacg atcaacgcca ttgagccaga attgcaggca
    19921 taacgcgtaa ttatcatgaa tgatttcggt ggtacaactt agtgcaatag tcggcggcaa
    19981 actaacctgt gcatcaacga catcatcttc ttcaatcgca acaaatagac gctcgctcgc
    20041 ctcaagcggg aaatattttc tcatcatcag gatttctctc cggctgcgtt ataaagcaag
    20101 gaatggcgct gaaaaaatgc ctttaaggtt ggaattaacg agagcgccac gtccagacat
    20161 tgcccaccga agtaagcaaa cgctgctccc agcacaccaa acagatgaca gaacatgaac
    20221 agcaatacga tgtagccgat tgaggcgatg gtttgcgcca cgagaaaagc gcgctgtttt
    20281 cccgccatca atagcaatga ctcctgcggg aagcccagca tcgaaatcac aattgcgccc
    20341 agcatcacct gaatcaggtc atacgcttcg agatacttaa cgccaaacac cagcgaaatg
    20401 agcggcttgc cgacgatcaa caccgcgagc gccaccagaa taccgattcc gcccgccagt
    20461 aaaccggact tcacacctaa caaccacggt ctggtggtgc gcggatctaa acgcatcacc
    20521 tctgggtaaa aacttttacc cagcaaaccc gctggcgttc cggcggcgtc gaagaatgtc
    20581 atggcgattt taaataaccc agcggcagcg ggtcctaaca cgatccccac caacactgtg
    20641 ctgcacgagt tacgcgccga ccagatggag tgggcaatgt tggttgacca gacaaaactc
    20701 cacgcgcctt taatgtgtcg ggcagactca aacagattca atttgaaggc gttatggata
    20761 tttctacggc gtaattcgcg cgcggcaaac caccagtaca tggtgccgcc aaccagattc
    20821 gacacgtacc aggcaataac aaaacccgca aaaccaaagt caaaatacca ggctgcgacg
    20881 ctccccgctg cgcgcagaaa aggtttcgtc gcctgctgta cagcaatcaa atcaaagcga
    20941 tctaccgcac gcagaatgcc ggtcggcgtg gaggaagcca ttgaaggaat gagcgtgcaa
    21001 tacagcgccg ccagccaaaa gctttggtca tccagaccta atgaatggga aaggaatggc
    21061 agtaaggcaa tgccgccgac aatcgccacc gcgccgctga cgatatccag cgagaaggaa
    21121 aatgagacga cattgcgaaa ttgctgcgga ttattgttgg tcaaggctgg cgttccgtac
    21181 tgaaccacca gctgccatgt ctgaaactta ataaaatcgc tgatcgactt ggcgtacgat
    21241 tgcacaatca ccagtacacc aaacatggcg ggcgtcatcc ctttaccggc acacgagagc
    21301 gccaacagcc ccagcaaggc gctctcgaca ttactggagc ctaaccaggc gctattgcga
    21361 ataatggtgc gaaacgcgcc atctgcaaac caatgtttga tgttaaaacc cgccaattca
    21421 gcctgacctt atcgttgata gtaaaaaata tcccgccagc cttaagttaa acttcggcgg
    21481 tcagaaacga tggcaaccag agaaaccgcc ttctgtacct gttccagcac ttcgctgtag
    21541 ggcgctctgg aatcaatctc aagaattttt gtgccgttat agccaatcgt cgacatgacg
    21601 ccgattttgt cctgcagctc ggcatactca tggtcaggct tgcgggagat ggcagtatca
    21661 atatcaatcc ccaaacgaat gattaattca ggacggtatt gcgccatttt ttggtataaa
    21721 ctccgttcgc gctgcgccag aaacatgctg attttcccgg tcgcacgttc gacgccgatc
    21781 cccggtccgt cataataaaa gcccgaaatt tcagcctgcg ggaagcgatc gctgaccacc
    21841 agaacgccac tttgcgccag tcgctgaacc ttgcgtagat tcgccattct tcgcagcgag
    21901 aagcagtaca taatcaccgc cgcccatagc gccggagatt tggttttcat gctttgggtt
    21961 ttcgaggatt tggccgccag tcgccgttcc agccagacgc caaccaacgg caatcgtttg
    22021 attttgtcgc cgtcttcgcc ggagagcagc cccagatagc ggcgctcggt ttgccagtgt
    22081 tgttgcagcg atttcaccag gtcggtggtc agcgtggatt taccggtgcc atcacacccg
    22141 accaccgcaa tcagccccgg aatgtagttg ggctgcggcg cggttgtact attaacagtt
    22201 tgtgtttgta gtgcatccat tcgttaaatc atcccctatc cattttggtg atcaggaaag
    22261 cgcggcgtga attgcgcgta gtgacgcttg cataatttca tcggctggtt gccgaccatc
    22321 cagttcaagg atcttggcgc cattgaatgt cagttgcggc gtaacggcga ttttttcctg
    22381 taacgctgcc agttggtggt caggtttacg cgcaaacgcg gtttgttcat caatgccaag
    22441 acgaatcaac aatacgggta aataagacgc catccattgg tacagcttca actcgcgctg
    22501 ccttaacatt tttatccaac cgttaccgcc cgtggttttt gccaattgcg ggccatcaaa
    22561 gcgaaaaccc ggcacttcaa cctgcgggta gcggtcggtg atgagcagat agccttgctg
    22621 gcttttacac aacattttgc gaaacttgta cgcccgccag caggaaagca gatagataac
    22681 cagcgcagta atattgccag gcggtgttga gggcttttcg tgtacatgtg ccgctttact
    22741 tcgcagataa cacccacaag gtgcgccaat aacagggagc tgtgaaatcc attcgccaat
    22801 tcgcccggac gattgcccga gataaatgtg ttctgttggc attcttgctg ccagttcatt
    22861 taccaggctt gccgtgaggg tcgatttacc tgaaccatca catcctacaa tggcaataac
    22921 acgcactggc gtggaattaa ttattgacat attacgttga ttcacgaaaa acccggcagt
    22981 aaataatgta ttgaatatta cgtggtccgt gctcagaata tccgttcaac cttattgtga
    23041 caatggttaa ttgtgacatt gacctgaatt acgctttcat aaaaacatat taaccaaata
    23101 aatattttta atggatattt aaattaaagg atatattcat gcagtcaata acacctccat
    23161 taattgccgt tattggtagc gatggttcag gcaagtcaac ggtgtgtgaa catcttatta
    23221 ccgttgtcga aaaatatggt gctgccgaaa gagttcattt aggaaaacag gccggaaatg
    23281 tcggtcgtgc agtgacaaaa ttacctttga tgggaaaatc cttacataaa acaattgaac
    23341 gaaatcaggt gaaaacagca aaaaaattgc ctggaccagt tccggcgctg gtaattacag
    23401 cgtttgtcgc ccgtcgctta ctgcgctttc gtcatatgct tgcctgtcgt cgtcgcgggt
    23461 taattgttct aaccgaccgt tatcctcagg accaaattcc tggcgcttac gatggtacgg
    23521 tgttcccacc taacgttgaa ggtggtcgtt ttgtctcatg gctggcaagc caggaacgta
    23581 aagcgtttca ctggatggcg agccataagc ctgatctggt catcaaactc aatgttgacc
    23641 ttgaagttgc ctgtgcacgt aaacccgacc ataaacggga atcgctggcg aggaagattg
    23701 ccataacgcc acagttaacc tttggtggtg cacaactggt tgatatcgat gccaatcagc
    23761 cactggaaca ggtgttggtt gatgcagaaa aagcgattac ggattttatg accgcgcgtg
    23821 gttatcacta gtcaaaaatg gaaatgcccg atcgccagga ccgggcattt tcaggaaggt
    23881 taaatcaact gcaatgctat ccagtacagc ccaccagaaa gaaaaattgc cgccggtaaa
    23941 gtaaacaccc acgccatcag gatgctggtt accgttttac gctgtaaccc accgccgtcc
    24001 accaccatcg tccctgctac cgcagacgag aggacgtgtg ttgtggagac aggcatccca
    24061 atataactgg caaggccgat agacactgcc gccgtcattt gtgccgccat gccttgcgca
    24121 tacgtcatgc cgcgcttacc aatcttctca ccgatggtca tcgctacacg acgccagcca
    24181 atcatggtgc caatgccgag cgccagtgct accgccatga tgatccacac cggagcgtac
    24241 tcaatggtgc ttaacatatc gctgcgcagt tttttcagca ggttctggtc ttctttactg
    24301 acgcctggca gtttcgccag cttcgcggag gtatcagaga tgcacagcat aatgcggcgc
    24361 agctggctgc gctgactcac gcttaacggc tcgtaacttt ccatattgcc tggcagcatc
    24421 gttttaacgc gcgcaatcgc atcaaaggta tttgccggat gacagtgaaa ctctgttact
    24481 tgcgtgccat cagtcgatgc tgcaggcaat ggaggttcca tcgcaatcaa cttctgcggc
    24541 agttcaggat gctgttgcag gtagtgttcg aagttggtaa cggcatcgcg ggtacgggta
    24601 atttcatagc cggacgcatt catattgacg acgaagccag caggggcaat ccccaccagt
    24661 accagcatta ccaggccgat ccctttctgc ccgtcgttca cgccgtgcga aaacgccacg
    24721 cctgcagcgg aaacaatcag cgcaatacgc gtccagaatg gcggtttacg tttgcccttt
    24781 ttctttttgc gatcttccgg aatgcggtga atacggtcac gctttttcgt cccactccag
    24841 aaacggcgca gcaggaaaat cagaccaccc gcaatgacca ggccgacgat aggggaaaca
    24901 atcagcgagg agaaaatttt ggttacttca cgcaggttta acgcatccat caccgatgag
    24961 ccggttaaca gcgcgttggt taaaccgatg ccgataatcg caccaatcaa ggtgtgcgaa
    25021 ctggaggccg gtaaaccaaa gaaccacgtt cccaggttcc agataatcgc cgccagcagc
    25081 atggaaaaga ccatcgccag accgtgagtt gaccccatat tcagcaacaa atcggttggt
    25141 aacatatgga caatggcata ggcaacgcta agtccgccca ataacacgcc aaaaaagtta
    25201 aaaaatgccg ccatcaccac agcaagttgt ggctgcatgg cacgagtata aataacggtt
    25261 gccaccgcat tcgccgtgtc atgaaaacca ttgattgctt cgtagaacaa cacaaatgcc
    25321 agagcaagta ataacaaaag ccccgtatat atatcaaggc caacaaataa atttagcata
    25381 tattagatta cgccattttg aatttacgaa cggacgcatt atcagtgact ttaacggcat
    25441 gggcaaagtg aaatatcatt ttttaacgat aaaaagagtg ttatttcttg tgtattagac
    25501 aataagcatt cattcaaaac ctttagaaat aatcaaaaca caggtattcc actggttaat
    25561 atattcacat atgaaatgaa taaatattgg aatatataaa tattgaatat tttgattaat
    25621 ccccgtacta attattcttc ataatcagta cggggttgct acaacatgta ttactttttc
    25681 accacaatta acggttcaat atcactctct tttttgatga ccagtgattc atcaccgcgc
    25741 aaacacgtcc caccgtagtt gccgccaacg gtgaaggtac atacctgaat gtatttaccg
    25801 tccactttcg gcaaacacca cagttgctga tagatgtttt tctgctcggc aaatttaccg
    25861 ctggttttgt ccagcacctc ttcatgatgg ccgaccaggt cgatattgct accacagcga
    25921 ccggcgatcg gtttcactgc gtaacctgtt ttcaccaatt catcattaac agtgaaatcg
    25981 gtatccagca ggtaacgatg gtgcgggaac agcgaccaga ggatcggcag aatcgctttg
    26041 ttgccgggga tcaccgtcca cagcggctca aagaccagca cttccgggcg cagcaatacg
    26101 tcgataagac gcacttcgtt ttgcggatga ccggtacgga ttggcaccgc agcaaactca
    26161 cggtcgctaa cttcacgaat ctgatcaaac gcggtttccc acgcccaggt tttccacacg
    26221 cagttaacca gtcgcccttc cccatcaatc agttgcccgg cagcatccca gcccagttca
    26281 tccagcccgc gcaagatacg cgtttcaaag cccgcctggt gcagcgcctg ctccataaac
    26341 tgcgcgtgat agttttcctc gatatctttg tcctgcatga tatggacaaa cggacgtgca
    26401 cgactgtgtt tccaggcacc agccagttcg ttaatcagcc cttccgccgg attgaagccg
    26461 ttgcctttat agccctgctc cgcccaacgt tcgaggatca agcccgcttc ggtatgacag
    26521 gaggcggagt cggcgttgta ttcgtaaacc ttcaggccac gctcatccat gcagaaatcc
    26581 atacgaccag tgatcatatg gtgacggcga cgctgccagg agagacgcaa acgtggccag
    26641 aggattttcg gaatgtcgaa cagcgccagc aggttgtcat ctttcagcac cttgtcggtt
    26701 gcgtgaagat acatcagatg cagctcgttg gtggctttaa ttagctcctg ctcggcactc
    26761 tcggtaatgg tgtagtaatg atacggatcc tgattgatca cctgaccgtt ggcctgcaca
    26821 taggcgtttt gtagcggatc tttttcatcc agccatttac cgtcaaactg gcctttgttt
    26881 tccagacgcg ctccactgat tttcagcagc tcgcctgcaa tttccggctg cggtaagctg
    26941 tattcagtat cttccgtctg gatcatccag cccagaatgg tggtgtcatc aaaagtgtct
    27001 ttaagggtat agcagccgtt ttcgaccacc atctccagct cgcgcgtcca ctgttgccct
    27061 tgcggcaacg gggaatgaat cacgttctgt tccgcaatac ggactttgtt gccatgcaat
    27121 tgggtaatga tggcgacatg gccagtgtct ttaaattcac cgcctttatc ccagataaga
    27181 agcgcacccg cgaccggcgc acgcggcgag ccgttaggaa atgcctgcaa tggcaggatg
    27241 ttgtcattaa ccacttctcg caggaagcgc agcgagaaaa tctcccacgc catacccacg
    27301 tcagtaaaga ccacaccgta attcagaaag agaaaacggc gagcaaattc aacgcattgc
    27361 cacttgtggc ccatatattc gtcgtcgata tagctacgga atacggcgtc atcttcgtat
    27421 tcctgcggat cgagagaact gtaatctgaa gagtagattg ctaccccacc tggggcgtag
    27481 cccaataatg tcccgaacgg ggcatcctgg ctggtcgttc ctttgctcat cactttacct
    27541 ttaacaatac aacctaagca gttggcgtaa ttgttgtgct ctgattacct gctcagcgac
    27601 attaaccgga cagaggtcag actaatcata cacctcatcg cagcggctgg cgcaaagggt
    27661 taaaaaattc acattctgta cagcaagtga cctgctacac tgcttcaaca ctaccactca
    27721 gaaggcaact cactatgaca gacaatactt atcagcccgc gaaagtctgg acgtgggata
    27781 aatccgctgg cggcgcgttc gccaatatca atcgcccgtt ttctggtccg acgcatgaaa
    27841 aaacgctgcc cgttggcaaa catccattgc aactttattc gctgggaacg ccgaacggtc
    27901 agaaagtaac gattatgctt gaggagctac tggcgctggg cgttactggt gcagagtacg
    27961 acgcctggct gattcgtatt ggcgatggcg atcaattctc cagcggcttt gtcgaagtga
    28021 acccaaactc gaagatcccg gcgctgcgcg atcatacgca taatccgccg atccgcgtgt
    28081 ttgaatctgg ttcgatcctg ctttatctgg cggagaaatt tggctacttc ctgccgcagg
    28141 atctggcaaa acggactgaa acgttgaact ggctgttctg gttacagggt gccgcgccgt
    28201 tcctcggcgg tggttttggt cacttttacc attacgcgcc ggtgaaaatt gagtacgcca
    28261 tcaaccgctt taccatggaa gccaagcgcc tgctcgacgt gctggataag caactggcgc
    28321 agcataagtt tgttgcgggc aatgagtaca ccattgcgga tatggcgatt tggccgtggt
    28381 ttggcaacgt ggtgctcggc ggcgtgtatg atgccgcaga gtttctggat gcgggaagtt
    28441 ataaacatgt gcaacgctgg gcgaaagaag tgggcgaacg tccggcagtg aaacgtgggc
    28501 gtattgttaa ccgcaccaac gggccgctga acgagcagtt gcatgagcgc catgacgcca
    28561 gtgattttga gacaaataca gaagataagc gtcaggggta agggttggtg ttcgtcgcag
    28621 caagccatcc aggccggata aggcgttcac gccgcatccg gcaatcgtgc acaatgcctg
    28681 atgcgacgct gccgcgtctt atcaggccta caaaagctaa tcatcacgcg ctggtaatgt
    28741 cgtaatccat ttggcgcagt gcgtctaatg tggctttggc ttcatcttcg tcgatgatgc
    28801 tcatggcaaa tccgacgtgt accagcaccc actggcccag cagatcggca gggttacctt
    28861 cgcaaatcag ggcgatattc acgtcgcgct tgataccaca tacttcaacc tgcgcaagct
    28921 ggtgaatatc ttcaccgaca gccagcacct ggcctggaac gccaatacac attgttaact
    28981 ccggttattt cgattgccgg acaaacgcga aaaagaacct ttagcgggtg ttccctctca
    29041 ccctaaccct ctccccggag gggcgagggg accgtccgtg cgctttttag gcgttgtcct
    29101 cagtcttatt caacttcaat acttttgacg atcagcgaat cgccggtatc gacacgcaac
    29161 cgctcgccgt gacagagcgg acactgcgca tcgtgctgat gaatctccac cacctggctg
    29221 caatcccagc accaggcctg ggcgggttta tagacgatat gtaaatcgca cccttgcgcc
    29281 accgttccgt ggcagacaat ttcaaaacta aaacggacgg cgctctcctc aacgcaggag
    29341 agcgcgccaa tttccagcca cacggcggtg acgcgcttaa catcgtgctg ctccgcctgc
    29401 cgttggataa tttcaacggc gctctggcaa agcgacaact catgcatttt cgcccctcct
    29461 gcggccaaac agcagggcgc gacgccctgc gtgtggtaca tccggattag tgactggcaa
    29521 cgaaaggatc attcgtgcgc agtcatccgt caggcgttgg ccttcttcaa tcgacatgct
    29581 gtgcgaaagc ggcgacatca gcgaacagga gaggtattgc gaaacaccgt ccagttcgcc
    29641 aacggtaaag gtcatggtgc catacggcag ttgcagacca attttttcac tcactttgcg
    29701 cagcggccag agttgatccg ggccggggaa gataactgca ctcagcatcc acggggtgat
    29761 cacacacccc gtccactgac cttcgaacag cgtaaaatca gaaacataca ccggcattga
    29821 aggatgcaga aaagagagat cgtgcatcga acgccgggca atttcttcaa acgctgcctg
    29881 tacttgcgcc ttcggggagg tctggaaacc tgctatctct tcagtcatga atcgcctccc
    29941 gtgggatggc ttccacgcct gattcgcgca gcgcagccag aaccagctca agcgcaggtt
    30001 caatcattgc ttcaaccgtc ggcgttaagc cgatgtgtgg ctccagcgat tccgggatca
    30061 cgccgaccag ggtcagcttt ttcggaaact caccggtgaa gcgcagggcc gacaatacgt
    30121 cggccaggcc aagctgatgc ggagagattt tgttggtaaa caacgccgga acttcttcat
    30181 cccgcaggat catcatcgtt cccggcgtgt tctttttcga cacaatggca tccgcaataa
    30241 tcagatgatc gcgatttgcc atgtcgccaa gcagctccat tcccgccgtg ccgccatcga
    30301 ggatctcaac ataatccggc agaatgtatc gttgctctaa cgcttcgaca atccgcacgc
    30361 cgatggcttc atcggtcagc aaaatattgc cgacccctaa gactaaaata cgcattacag
    30421 aaccttcact gaaaccactt cgttgccgtc ggcatccact acgtgtaccg cacaggccat
    30481 gcacgggtcg aaggagtgaa tggtacgcac cacttccagc ggtttattcg gatcggcaac
    30541 cggtgtaccc accagcgact gctcgtaagg accgacgtca tcattgaagt tacgcggacc
    30601 agagttccag gttgatggaa caaccgcctg gtagttgctg atgataccgt ctttaataac
    30661 catccagtga gagagcatac cgcgcggcgc ttcgaggaag ccaacacctt tgaactcacc
    30721 cgttgccgga atgttcggct tcacaaaggt ggtgtgatcg cctttgccga tattggtgat
    30781 cagtgcactg tattggtttt gcaggatatc ctgcaattca cagcagtgaa cggtacgacc
    30841 aataatacgg cccagcgtgg agtgcagctg tgccacttcc agcgtgttgc cagtcagttt
    30901 ctgataaatc gcaacgattt cattcagttt gttctgggta gattcgcgac ctgccgccag
    30961 tttcaccagc atattagcca gcggacccac ttctaccgtt ttgccgtaga aagtcggtga
    31021 tttcacccag gaatatttcc cgtcgtcaga ccaaccatca taagccggaa tggtggtacc
    31081 ttcccatggc gcctgtggcg cttcgtcttt ataccaggag tgcttcgcgc tttcctgaat
    31141 gcctttgatc aggtattcat cggaatgaga agtgatcgga cgatacgagg acagatccgc
    31201 attctcaatg tagccgcccg ggaacaggaa gctgccgttt ttgctgtcgg tcgggaattc
    31261 cggcacgctc aggtagttca ccgcaccttt accgcgcgtc agccattccg ggtagaacgc
    31321 ggcgataacc gcggtatcaa ccttataaac ctgctcaaca aagtcgctca gtttgtcgat
    31381 gaaagacttg atgtacatca ggcgctcaag gttcaacacg cccaacccgt cgaggttgat
    31441 tgggttcgcg acaccactca ctgccaggtt ctgaatgtgc ggcgttttac cgcccagcag
    31501 cgccacgaca cggttagcgt cacgctggca ctccagcgcc tgcaggtagt gcgctaccgc
    31561 aatcaggttc acttccggcg gcagtttcat cgccgggtga ccccagtagc cattggcgaa
    31621 aatacccaac tgaccgctgg caaccagatc tttgatcttg ttctgaactt tagtgaactc
    31681 ttccggactg ttcagatgcc aggtcgaaac gcctttcagc atttcggagg ctttggttgg
    31741 gtcagcttgc agtgcagaag tgatgtccac ccagtccagc gccgaaagct gatagaaatg
    31801 aacgatatgg tcatgcgtgg tgtgcgcagc cagaatgatg ttacggatgt attgcgcgtt
    31861 aaccggaacg tcgatattca gcgcactttc tgccgcacga acggaagaca gcgcgtgagt
    31921 ggtagtacat acgccacaga tacgttgcac aatcatccat gcatcgcgcg gatcgcggtt
    31981 tttcacgatc tcttccatgc cgcgccacat ggtaccggaa gcccatgctt tcgaaacgac
    32041 gccattttcg atttcgcaat cgatgcgtaa atgcccctca atacgggtta ccggatcaat
    32101 agtaattctc tggctcatgc tttgctcgcc tcatgacgat tatgatcgtt ttgttttaaa
    32161 ggaggaagta tcggcagtag acgaatgagt acgatgtaag cgcaaatctc aatagccaca
    32221 aaaccaatag aaatcaacag ttcttcccag gtcgggaagt aggcgtaacc gccgcccggg
    32281 ttgaatgcca ccaacgaata ggtcagacgc caggttgcac aacctaacag tgcgctcagt
    32341 gctgacagga acagcatgcg ggaatcatta cgcagcttcg ccacacgcag aacgaccagc
    32401 gggaagagca tcagcaggac ttcaatccag aacatcacgg agtagaagtc accggcaaac
    32461 gctaacgaca gcttgtcgcg atagataagc tcgccaaagc gcagcccgat gaaaatcgcc
    32521 agcaacacac tgatggtgtt ggtcagcttg acgaacaggc tcttttcatc cggaccgttg
    32581 ccacgcagac ccgcctgcac cagtgaacct tcaaagatga caatcgagaa gcccatgatg
    32641 aacgccgtca gcagcgagaa cagcggcaac atttcatagc tctgccagag cggatgcacc
    32701 ttgtagcccg ccgagatcat cagcgacccc attgaagact ggtgcatggt cggcagcagc
    32761 gcaccgagcg cgatgatgaa gaacatcacc ttgttcagac gttgtagcga caccttccag
    32821 cccagacgtt caaacagtgc cggagcaaac tccagagcca tcacgccgat gtagatggtc
    32881 atacagaccg ccgtctcgaa cagtaccgag ttcacgttga agtgacccgg aatgtagaag
    32941 tacggcaggt tccagtagcg acccacgtcg atagtgatcg acaagccacc cagtgagtaa
    33001 ccaaacagac tcgccaacag cgccggacgc accagcggat ggtattgccc acggttaaag
    33061 acgtataccg cccacgccag cgcccagccg ccacaggcaa agccggtgcc aatcagcagg
    33121 tcaaacgcga tccacacgcc ccacgggaag ccgccgttca ggtcagagac agagcccaga
    33181 ccgaacacca gacgcttcac aatcaggagc atacagatga cgattaacgg tccaaaaatc
    33241 atgaccggtt tactgatgat tttgccgccc agcggttgtg gatcatgact catgatcgtc
    33301 tcctccgtcg tgatggtcgt ttttggtgtt gcgacgaacc agcacggtta agcccgccag
    33361 cacagccagt ggtagcatca tgcctttata cagggtgtgt tgaatatttt cggaacgcgc
    33421 accggtagaa agatcgtcca gtttcggcag gtcgagattt tcataaggca cacccgtcag
    33481 taccagaacc tgagtaccgc cgccctcttt ctcgccgtac agatgcggat aatatttcgg
    33541 caccgtatgc aggtaagtgt cgccagattt cagcgtctga cgcggatagt ggtattcgct
    33601 gccaggcttc agcgccagac gttttttcgc ctccgccatc agctcttcac gcgtaccgaa
    33661 aatcaccgcg cccgccgggc acacttctac gcagccaggc agaccgcctt tatcgagacg
    33721 ttccacacct ttctggttgc acagctcgca cttatgcagc gcaccaaacg ggttgttgta
    33781 gtcgtacttc ggcacgttgt acggacaggc gaccatgcag taacggcaac cggtgcacac
    33841 gtctttgtcg taatggacaa tgccggtttt cggatctttt ttcagtgcag agaccgggca
    33901 cacagagaca cagttcggat cgacgcagtg catacactgt ttcttaatgt acgcgtagcc
    33961 gttctcctcc tggtctttgt tgacccctgt gccgctggtc cacacctgaa tgatgttatt
    34021 ggtatacggc gacagtttgt cgttgttcga ccaggtctgt tccccttgcg ggttacgttc
    34081 agggaagttg atatcctgac acttggtgac gcaagcctgg cagcctacgc acaaggtcga
    34141 gtcgtacaac atccccagcg atcccggaat tggcgggcgg ttttcagcag ccgcatgact
    34201 gacagacggc agcgcgcccg tcagcaatgc cccgcaggag gctgctttaa taaaattacg
    34261 tctgttcacg gttattctcc ccgtgagtca gcgttatctt tcttttgctg acgacccagt
    34321 tcacgcaccg ccatcacgct gacaccggca accagcccaa ccacaccgcc gagcaaacca
    34381 atagcgcctg cagagacgtt gccgccctct ttagcgttaa catccggttt ctgtgaacgc
    34441 ggagtctgat tttcgacgtt ggcaagctga tggatgcctt tatggaagcc gatacctttt
    34501 tcgttacagc catagcacgg gtgaccaatc gccaccggcc acacaccgcc aacatcgcag
    34561 aattgcagcg ttgagcagtt gccgtaagtt tctggccctt tacagccgag gtggtacagg
    34621 caccagcctt cgcggtggcc ttcatcaccg aactctttgg caaaacgacc agcatcgaag
    34681 tgcgggcgac gttcgcagtg ttcgtgaatc agacggccat aggcgaaggt cggacggttt
    34741 ttgtcatcca gtttcggcgg tttgccgtaa gtgatgatgt gcgcaacggt cgcgaggaag
    34801 ttgtgcgggt tcggcgggca gcccggaata ttgataacgg ttttgcctgg cagaacttct
    34861 tgcaagctga ctgcgccagt tgggttaact ccagctgcgg caacaccgcc ccacgcggag
    34921 caggaaccaa tagcgataat ggctgctgcg ccttccgccg ctttgcggat gtgatccaca
    34981 atcggctcac cggcaaccat gcaataaata ccgttatctt ttaatgggat ggaaccatcc
    35041 accactaaca catactgccc tttgtacttc tcgagagcgt tatgtttgtt ctcttcgacc
    35101 tgatgaccga aggcggcgga aagcacttcg tgatattcca gagagatagt ctccagcacg
    35161 aggttttcta ccgttggatg cgttgcacga agcagagatt ccgtacaacc ggtgcactcc
    35221 tgcgcgccaa tccagataac tggcggacgc tgcgggttag taaccgattc ggccatctct
    35281 gcagcggctt tgctacttaa ccccatggtg gcggctaatg ctgcacaaag cttcatgaaa
    35341 tcacgacggt taatgccgtg agaatggatg agggtgttat ctccagtcat ttatagttat
    35401 tccgttgcga ggacctggca tatattttgc ctcaatcgca aaatcaataa tgcgatcgat
    35461 gcgccattta ccacacattt attatggtta tcgatatcat gatactgcgg cggggcgcta
    35521 aaacgaaggg aagaagcatt agtgtaatta attaaagcaa gataatacgt atgtttgatc
    35581 aattttcgag gcgataaccg gccataaaaa agcgcaacgt agaacaggaa ttattctcac
    35641 gaaagaaatc cattaccatc tctttatcca gtccatagcg gcgcgtcaga atacccgctt
    35701 cccaggcgct aagctggcga tctccggtct tttcgtacat ccggtgtgag tagcctaata
    35761 caaaaccgcg tttataatcg gcacagaatt ttgtcacatt aaccgcactg tcggcatgcg
    35821 ttgcttttaa tcccgccatt aagcctttac caaaatggtt attcatcgtt cacctcattc
    35881 gttatatatc gccatatata gcgatatttt aggcaaagaa cgacttttgc gcacgctatt
    35941 ttttcatact tgtttaagac gtaatcccgt aagagataaa cctatcagga gagatattta
    36001 tcgaaccatt tcagtgtcct ttgccaggca agatcggcgg cagatttgtc ataacggggc
    36061 gtggaatcat tatggaatcc gtgattaacc cccggataga tataagcctc ataaacctta
    36121 ttattgtctt tcaacgccgc ctcgtaagga ggccagccct cgttgattcg ggtgtccagt
    36181 tccgcgaagt ggagtagtaa aggcgcttca atcttcgcca catcggcagt gggtgcctga
    36241 cgaccataaa acggcaccgc gcaggccagt tccggatacg cgacagccgc cgcgttcgat
    36301 acgccaccgc catagcaaaa tccggtaata cccactttac ctgtcgcttg cggatagcgt
    36361 tgcataaact caattgcggc aaagaaatca ttcatcagtt tggttggatc aacctgctgt
    36421 tgcagctcac gacctttatc atcatttccc ggataacctc caacggaact taatccgtca
    36481 ggtgccaggg cgatataccc cgccttcgcc actcgccgtg ccacatcttc gatatacgga
    36541 ttcagtccac gattctcatg caccaccccc acggctggcg ttttgccgct catttttgcg
    36601 ggtttcacca gataaccccg tacctcgccg tgaccatttg gcgaaggata cgtgatgtac
    36661 tcagcaacaa tctccgggtc ggtaaactct acctgagtcg ccagcgcata atttggcttg
    36721 agcaaatcaa acaacgctaa tgccgtcatc ccgcccaccg catacttcgc cgcaaggttg
    36781 aggaactcac gtttcgaaat tttcccgtga acgtaatagt cgtagtaatc caacaactct
    36841 tgtgggaaat ctttggcggt taaacgcggc atcgttgcat tcctcaactg gtgttttttt
    36901 aagcaaagca taagcgcgtg tttttgccca gtttttcgtc actctgtgag ccagactacg
    36961 ggatacgcgc tggcgaatcg ctaaactaga aacattgttt cgaaattgaa cggtggaaag
    37021 gagaggtcat ggtctggtta gcgaatcccg aacgttacgg gcagatgcag taccgctatt
    37081 gcggaaaaag tggtttacgc ctgcccgcgt tatcgctcgg tttatggcac aatttcggtc
    37141 acgttaacgc gctggaatca cagcgtgcaa tcctgcgtaa agcgtttgat ttaggcatta
    37201 cgcactttga tttagccaac aattacgggc cgcctccagg aagcgcagaa gagaactttg
    37261 gtcgcctgct gcgcgaggat tttgctgctt atcgcgatga actgattatc tctaccaagg
    37321 ctggctacga tatgtggccc ggcccgtacg gctctggcgg ttcacgtaaa tacctgctcg
    37381 ccagcctcga ccaaagccta aagcgtatgg ggcttgagta tgtcgatatc ttttactctc
    37441 atcgcgtcga tgaaaatacg ccgatggaag aaaccgcctc tgcgctggct catgcggtac
    37501 aaagcggtaa ggcgctgtat gtcgggatct cctcttactc gccagagcgg acgcaaaaaa
    37561 tggtcgagtt gctgcacgag tggaaaattc cgcttttaat tcatcaacct tcgtacaatt
    37621 tactgaaccg ctgggtggat aaaagcggcc tgctggatac cctgcaaaat aacggcgtgg
    37681 gctgtattgc ctttactcct ctggctcagg gattgctgac cggaaaatat ctcaacggca
    37741 ttccgcaaga ttcacggatg catcgtgaag ggaataaaat tcgtggtctg acgccgaaaa
    37801 tgcttaccga agccaacctc aacagcctgc gcttattgaa tgaaatggca cagcagcgtg
    37861 gacaatcaat ggcgcaaatg gcgttaagct ggttgctgaa agatgatcgc gtgacgtcgg
    37921 tattgattgg tgccagccgc gcggagcaac tagaggagaa cgtgcaggcg ctgaataatc
    37981 tgacatttag caccgaggag ctggcgcaga ttgatcagca tatcgccgat ggcgaactga
    38041 atctgtggca ggcgtcgtcc gataaatgac ctgttaataa cgggcgcgcg aaccgcgccc
    38101 gaatcagatc agtgattatg acgagtcagt cggtcaagat agcccatcac aaatgcagaa
    38161 agcacaaacg tcagatggat aatgacgtac cacatcagtt tgttatccgg gacatttttc
    38221 gcatccataa agacgcgcag taagtgaatg gaagaaattg ccacaatcga cgctgctact
    38281 ttgtttttca gcgacgttgc gtccattttc cccagccagt tcagcttctc tttgttctcg
    38341 gagatatcca gctgtgagac gaaattctca taaccggaaa acatcaccat caccagcaaa
    38401 ccgccaacca gcgtcatatc caccagcgac agcaacacga ggatcaaatc tgattccgcc
    38461 atcgagaaga tattcggcag tacgtgaata atctcctgga agaacttcag cgccagggca
    38521 actaacgcca gcgaaaggcc aaagtacacg ggggcaagca gccagcgaga agcatacatt
    38581 gcattttcaa gaaaacgttc catacattcc tgtcgtgtag ttaaaacggg caacagtata
    38641 tcgcaattga gtaaacagca cgcaacagca aatcaacata tacgttaaac ctttgtcgca
    38701 taattctgag gtttagggat cttcctggtt cccttatcaa cgcatcgaga gcgtctaccc
    38761 ttaaaggacg ttgaacctga agggagaaaa acgatgtctc atttaaaaga cccgaccacg
    38821 cagtattaca ctggtgaata tcccaaacag aaacaaccga cgccaggcat ccaggcgaag
    38881 atgacaccga taccggattg cggcgagaaa acctatgttg gtagcgctcg cctgaaagat
    38941 cgtaaagcac tggtgacagg gggcgattcc ggaataggtc gcgctgccgc catcgcttac
    39001 gcgcgagaag gggctgacgt ggcgatcagt tatcttcccg tggaagaaga agacgctcag
    39061 gatgtgaaaa agatcattga agaatgcgga cgcaaagccg ttctgctgcc aggcgattta
    39121 agcgatgaga aattcgcccg ttcgctggtt cacgaagcgc acaaggcgtt aggcgggctg
    39181 gatattatgg cgctggtcgc cgggaaacag gttgccattc cggatattgc agacctcacc
    39241 agcgaacagt ttcaaaagac ctttgccatt aacgttttcg cgctgttctg gctaacccag
    39301 gaagcgatcc ccctgctacc gaaaggtgca agtatcatca ccacttcgtc aatccaggca
    39361 taccagccaa gtccgcattt actggactat gcggctacga aggcggcgat tctgaactac
    39421 agccgtggcc tggcaaaaca ggtcgcggag aaaggtattc gggtgaatat tgtcgcgcca
    39481 ggccctatct ggacggcact gcaaatttcc ggcggacaaa cgcaggataa gatcccgcag
    39541 tttggtcaga aaacgccgat gaagcgtgcg gggcaaccgg cggaactggc ccctgtatat
    39601 gtttatctgg caagtcagga gtcgagctac gtcaccgcag aagtgcacgg cgtgtgcggc
    39661 ggcgagcatt taggttaaaa aaatgcccgg ttgtgaaaag caaccgggca tcattgtgaa
    39721 ttcactctta cctgatgtgg cgaaaacgcc ttatccgcct acaaacgcat gaagattcaa
    39781 catgttgcaa tagattttgt aggcctgata agcgtagcgc atcaggcatt gagcacctaa
    39841 tgccggatgc gtcgtgaacg ccttatccgg cctacaaacg cacgaagatt caacatgttg
    39901 caatagattt tgtaggcctg atgagcgtag cgtatcaggc aattctactt tacttcgctt
    39961 tggcggtttc ttcgccgacc agacctatct tcaggtaacc cgcctgatgc agcgtatcca
    40021 ttaccttcat caacgtctcg taatcgacgg ttttgtccgc tcggaagaag atggtggtgt
    40081 ctttcttgcc ttcggttaac gcattcaacg ccgtaatcat tgtttcatcg gtgaccggat
    40141 cgttaccgat aaacatcgag ttgtctgcct tcaccgacag ataaaccggt ttttccgggc
    40201 gcggctgcgg cgtgctggta gaagcaggca agttcacctt cacatctacc gtcgctaacg
    40261 gtgccgccac cataaagata atcagcagaa ccaacatcac gtcgataaac ggcgtcacgt
    40321 tgatatcatg catttcgccg ttatcgtcga ggttttcgtt aagatgcatt gccatcggac
    40381 attatcctgc gcgtaatttt tgtgcgacac gaaccggatg cgcagcggcg ctggcttcca
    40441 gatccaggtc acggctttgc agcaacaata cctgcgctgc aacatcaccc agcatcgctt
    40501 taaagccgcc aatctggcgt gcaaatacgt tatagataac gaccgcagga atcgctgcca
    40561 cgaggccgat tgccgttgct aacagagctt ctgcgatacc cggcgcaacg actgccaggt
    40621 tagtggtctg cgtttgcgcg ataccaataa agctgttcat gatgccccag accgtaccaa
    40681 acagaccaac gaacggagaa atcgcgccga tggttgccag atagccgtta ccgcggccca
    40741 tttgacgacc cactgcggcg acccgacgct ccagacggaa gctggtacgt tctttaatac
    40801 cttcgttatc gtcgctgcct tctgacagtt ccagctcatt ctgcgcttca ttgagcaaat
    40861 gcaggcttaa gcttttgcta ccaaaatcag cggcgatatc gttggcctgg tttaaggaac
    40921 gtgcttcagc cagcagttgc tgctcgcgct taaggcgacg cttctgattg aagaactcta
    40981 cgctcttact gaagaagatt gcccaggtga ctacggaggc caaaataagc ccaatcatca
    41041 cgcacttaac gacaatatcg gcgtgctgat acatacccca gacggaaagg tccgtctgca
    41101 ttaaattatt acccactgtg tatctccagg acgcaagtca caaaatctgc gcataataat
    41161 atcaaaacga cgtcgaattg atagtcgttc tcattactat ttgcatgctg ccgtaccttt
    41221 gctttctttt ccttgcgttt acgcagtaaa aaagtcacca gcacgccatt tgcgaaaatt
    41281 ttctgcttta tgccaattct tcaggatgcg cccgcgaata ttcatgctag tttagacatc
    41341 cagacgtata aaaacaggaa tcccgacatg gcggacaaaa agcttgatac tcaactggtg
    41401 aatgcaggac gcagcaaaaa atacactctt ggcgcggtaa atagcgtgat tcagcgcgct
    41461 tcttcgctgg tatttgagag tatggaagcc aaaaagcacg cgacgcgcaa tcgcgccaat
    41521 ggcgagttgt tctatggacg gcgcggaacg ttaacccatt tttccttaca acaagcgatg
    41581 tgtgaactgg aaggtggcgc aggctgcgcg ctatttccct gcggggcggc agcggttgct
    41641 aattcgattc ttgcttttgt cgaacagggc gatcatgtgt tgatgaccaa caccgcctat
    41701 gaaccgagtc aggatttctg tagcaaaatc ctcagcaaac tgggcgtaac gacatcgtgg
    41761 tttgatccgc tgattggtgc cgatatcgtt aagcatctgc agccaaacac taaaatcgtg
    41821 tttctggaat cgccgggttc catcactatg gaagtccacg acgttccggc gattgttgcc
    41881 gccgtgcgca gtgtggtgcc ggatgccatc attatgatcg acaacacctg ggcagccggt
    41941 gtgctgttta aggcgctgga ttttggcatc gatgtttcta ttcaggccgc caccaaatat
    42001 ctggttgggc attcagatgc gatgattggc actgccgtgt gcaatgcccg ttgctgggag
    42061 cagctacggg aaaatgccta tctgatgggc cagatggtcg atgccgatac cgcctatata
    42121 accagccgtg gcctgcgcac attaggtgtg cgtttgcgtc aacatcatga aagcagtctg
    42181 aaagtggccg aatggctggc agaacatccg caagttgtgc gagttaacca ccctgccctg
    42241 cctggcagta aaggacacga attctggaaa cgagacttta caggcagcag cgggctattt
    42301 tcctttgtgc ttaagaaaaa actcaatgat gaagagctgg cgaactatct ggataacttc
    42361 agtttattca gcatggccta ctcgtggggc gggtatgaat cgttgatcct ggcaaatcaa
    42421 ccagaacata tcgccgccat tcgcccacaa ggcgagatcg attttagcgg gaccttgatt
    42481 cgcctgcata ttggtctgga agatgtcgac gatctgattg ccgatctgga cgccggtttt
    42541 gcacggattg tataacattg ccacttttgg acaattttgc agacatttta ttgtgaaaag
    42601 tcttaaattg ttgcgtccgg gatcaaggcg tcccggacga ttcaggagta caataggcag
    42661 ataaaggctt aaacgctgtt ccacaggaaa gtccatggct gttattcaag atatcatcgc
    42721 tgcgctctgg caacacgact ttgccgcgct ggcggatcct catattgtta gcgttgttta
    42781 ctttgtcatg tttgccacgc tgtttttaga aaacggcctg ctgcccgcct catttttgcc
    42841 aggcgacagc ttgttgatac tggcaggcgc attgattgcc caaggggtta tggattttct
    42901 gcctacgatt gcgattctga ccgccgcagc aagtctgggc tgctggctaa gttatattca
    42961 ggggcgctgg ttagggaata ccaaaacggt gaaaggctgg ctggcacagc ttcctgctaa
    43021 atatcaccag cgcgccacct gcatgtttga ccgccacggt ctgctggcgc tgctggctgg
    43081 acgttttctt gcatttgtcc gtacgctgct gccaaccatg gcgggaattt ccggtctgcc
    43141 aaaccgccgc ttccagtttt tcaactggtt aagtggattg ctgtgggtca gcgtggtaac
    43201 cagttttggc tatgccttaa gtatgattcc gttcgttaaa cgccatgaag atcaggtaat
    43261 gacgttcctg atgatcctgc caattgcctt gttaaccgct ggcttgttag gcacgctgtt
    43321 tgtggtgatt aaaaaaaaat actgtaacgc ctgacgattt tccccgttcc cggctgctgt
    43381 accgggaacg tatttaattc ccctgcatcg cccgcattct tgccgcatct tcccccggcg
    43441 tcacaccgaa gtaacgttta aactcacggc taaattgcga tgcgctttca tagccgacgc
    43501 gcatcgctgc tgcgctggcc ttcatgccgt catggatgat catcatccgc gccttatgca
    43561 gacggtaatt cttcaaatac tgcaacggcg aggtgctggt gacagactta aaattatggt
    43621 ggaacgccga tacgctcatg ttggcttctg ccgccagttg ctcgacgctc aggttttcgg
    43681 tgtatttatt ctcaattcgt ttcagcacgc ggctaatcag actgaagtga gtctggcgac
    43741 tgaccagcgc cagtaacgcg ccgccgcaag gtccggtcag cacgtagtac agaatttcgc
    43801 ggatgatctg tttgccgaga atacgcgcat ccagtggtcg ctccatcacg tcgagtaacc
    43861 gctccgccgc gaataaaatc tcttctgata acgtggcgga gttaatcccg ctggctgcca
    43921 tcgacggctg gaaatgctca tcttcgccaa tgtccatcaa cagttcctgt aactgcaaaa
    43981 tatcgacatt gagacgcaac cctgccagcg gcacctctga cgtggcatag gtttcgcact
    44041 caaacggcaa cggcaccgtc agcagcaggt attcattggc atcataacga aacacgcgtt
//
LOCUS       DADMEQ010000019        42885 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.019, whole genome shotgun sequence.
ACCESSION   DADMEQ010000019 DADMEQ010000000
VERSION     DADMEQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42885
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.019"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..413
                     /locus_tag="JNZ05_001107"
     CDS             <1..413
                     /locus_tag="JNZ05_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY8564420.1"
                     /translation="MYIGAPLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKL
                     FWRPKGLAYPLGEAPAKLRCTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFD
                     KYQFSLPKGAKNSSVTYRTINDYGAETPQLNCKS"
     gene            438..3037
                     /locus_tag="JNZ05_001108"
                     /pseudo
     CDS             438..3037
                     /locus_tag="JNZ05_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3028..4098
                     /locus_tag="JNZ05_001109"
     CDS             3028..4098
                     /locus_tag="JNZ05_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8564421.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESSDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4110..4652
                     /locus_tag="JNZ05_001110"
     CDS             4110..4652
                     /locus_tag="JNZ05_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8564422.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLIYQ"
     gene            4660..5175
                     /locus_tag="JNZ05_001111"
     CDS             4660..5175
                     /locus_tag="JNZ05_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY8564423.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5141..5878
                     /locus_tag="JNZ05_001112"
     CDS             5141..5878
                     /locus_tag="JNZ05_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HAY8564424.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRHEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5989..6999
                     /gene="pyrD"
                     /locus_tag="JNZ05_001113"
     CDS             5989..6999
                     /gene="pyrD"
                     /locus_tag="JNZ05_001113"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HAY8564425.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7173..7715
                     /gene="zapC"
                     /locus_tag="JNZ05_001114"
     CDS             7173..7715
                     /gene="zapC"
                     /locus_tag="JNZ05_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HAY8564426.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7712..8821)
                     /gene="ycbX"
                     /locus_tag="JNZ05_001115"
     CDS             complement(7712..8821)
                     /gene="ycbX"
                     /locus_tag="JNZ05_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HAY8564427.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            9065..11173
                     /gene="rlmKL"
                     /locus_tag="JNZ05_001116"
     CDS             9065..11173
                     /gene="rlmKL"
                     /locus_tag="JNZ05_001116"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HAY8564428.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPSATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11185..13092
                     /locus_tag="JNZ05_001117"
                     /pseudo
     CDS             11185..13092
                     /locus_tag="JNZ05_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            13222..14475
                     /gene="pqiA"
                     /locus_tag="JNZ05_001118"
     CDS             13222..14475
                     /gene="pqiA"
                     /locus_tag="JNZ05_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HAY8564429.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQLLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14480..16120
                     /gene="pqiB"
                     /locus_tag="JNZ05_001119"
     CDS             14480..16120
                     /gene="pqiB"
                     /locus_tag="JNZ05_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HAY8564430.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16117..16680
                     /gene="pqiC"
                     /locus_tag="JNZ05_001120"
     CDS             16117..16680
                     /gene="pqiC"
                     /locus_tag="JNZ05_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HAY8564431.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16936..17103
                     /gene="rmf"
                     /locus_tag="JNZ05_001121"
     CDS             16936..17103
                     /gene="rmf"
                     /locus_tag="JNZ05_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HAY8564432.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17173..17691)
                     /gene="fabA"
                     /locus_tag="JNZ05_001122"
     CDS             complement(17173..17691)
                     /gene="fabA"
                     /locus_tag="JNZ05_001122"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HAY8564433.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17760..19520)
                     /locus_tag="JNZ05_001123"
     CDS             complement(17760..19520)
                     /locus_tag="JNZ05_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HAY8564434.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPHWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19705..20157
                     /gene="matP"
                     /locus_tag="JNZ05_001124"
     CDS             19705..20157
                     /gene="matP"
                     /locus_tag="JNZ05_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HAY8564435.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20233..21279)
                     /gene="ompA"
                     /locus_tag="JNZ05_001125"
     CDS             complement(20233..21279)
                     /gene="ompA"
                     /locus_tag="JNZ05_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HAY8564436.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21635..22144)
                     /gene="sulA"
                     /locus_tag="JNZ05_001126"
     CDS             complement(21635..22144)
                     /gene="sulA"
                     /locus_tag="JNZ05_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HAY8564437.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22363..22992
                     /gene="sxy"
                     /locus_tag="JNZ05_001127"
     CDS             22363..22992
                     /gene="sxy"
                     /locus_tag="JNZ05_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HAY8564438.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(22955..25117)
                     /gene="yccS"
                     /locus_tag="JNZ05_001128"
     CDS             complement(22955..25117)
                     /gene="yccS"
                     /locus_tag="JNZ05_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HAY8564439.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQAPQETPVSA"
     gene            complement(25127..25573)
                     /locus_tag="JNZ05_001129"
     CDS             complement(25127..25573)
                     /locus_tag="JNZ05_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAY8564440.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            25696..27750
                     /gene="helD"
                     /locus_tag="JNZ05_001130"
     CDS             25696..27750
                     /gene="helD"
                     /locus_tag="JNZ05_001130"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HAY8564441.1"
                     /translation="MELKATTLGKRLVQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(27782..28240)
                     /gene="mgsA"
                     /locus_tag="JNZ05_001131"
     CDS             complement(27782..28240)
                     /gene="mgsA"
                     /locus_tag="JNZ05_001131"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAY8564442.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28336..28998)
                     /locus_tag="JNZ05_001132"
     CDS             complement(28336..28998)
                     /locus_tag="JNZ05_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HAY8564443.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29171..29584
                     /locus_tag="JNZ05_001133"
     CDS             29171..29584
                     /locus_tag="JNZ05_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAY8564444.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29629..29946)
                     /gene="hspQ"
                     /locus_tag="JNZ05_001134"
     CDS             complement(29629..29946)
                     /gene="hspQ"
                     /locus_tag="JNZ05_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HAY8564445.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30004..31194)
                     /gene="rlmI"
                     /locus_tag="JNZ05_001135"
     CDS             complement(30004..31194)
                     /gene="rlmI"
                     /locus_tag="JNZ05_001135"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HAY8564446.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31289..31567
                     /gene="yccX"
                     /locus_tag="JNZ05_001136"
     CDS             31289..31567
                     /gene="yccX"
                     /locus_tag="JNZ05_001136"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAY8564447.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31564..31893)
                     /gene="tusE"
                     /locus_tag="JNZ05_001137"
     CDS             complement(31564..31893)
                     /gene="tusE"
                     /locus_tag="JNZ05_001137"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HAY8564448.1"
                     /translation="MLIFEGKEIEMDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(31984..32643)
                     /gene="yccA"
                     /locus_tag="JNZ05_001138"
     CDS             complement(31984..32643)
                     /gene="yccA"
                     /locus_tag="JNZ05_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HAY8564449.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(32850..32937)
                     /locus_tag="JNZ05_001139"
     tRNA            complement(32850..32937)
                     /locus_tag="JNZ05_001139"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32901..32903),aa:Ser,seq:tga)
     gene            33363..34481
                     /gene="hyaA"
                     /locus_tag="JNZ05_001140"
     CDS             33363..34481
                     /gene="hyaA"
                     /locus_tag="JNZ05_001140"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HAY8564450.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLYYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPSCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            34478..36236
                     /gene="hyaB"
                     /locus_tag="JNZ05_001141"
                     /pseudo
     CDS             34478..36236
                     /gene="hyaB"
                     /locus_tag="JNZ05_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
     gene            36255..36365
                     /locus_tag="JNZ05_001142"
                     /pseudo
     CDS             36255..36365
                     /locus_tag="JNZ05_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase B-type cytochrome subunit"
     gene            36400..36987
                     /locus_tag="JNZ05_001143"
     CDS             36400..36987
                     /locus_tag="JNZ05_001143"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HAY8564451.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGRLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            36984..37382
                     /gene="hyaE"
                     /locus_tag="JNZ05_001144"
     CDS             36984..37382
                     /gene="hyaE"
                     /locus_tag="JNZ05_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HAY8564452.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            37379..38236
                     /locus_tag="JNZ05_001145"
     CDS             37379..38236
                     /locus_tag="JNZ05_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HAY8564453.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINSTGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            38370..39914
                     /gene="appC"
                     /locus_tag="JNZ05_001146"
     CDS             38370..39914
                     /gene="appC"
                     /locus_tag="JNZ05_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HAY8564454.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            39926..41062
                     /gene="appB"
                     /locus_tag="JNZ05_001147"
     CDS             39926..41062
                     /gene="appB"
                     /locus_tag="JNZ05_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY8564455.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41074..41166
                     /gene="cbdX"
                     /locus_tag="JNZ05_001148"
     CDS             41074..41166
                     /gene="cbdX"
                     /locus_tag="JNZ05_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HAY8564456.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDLRLKS"
     gene            41246..42544
                     /locus_tag="JNZ05_001149"
     CDS             41246..42544
                     /locus_tag="JNZ05_001149"
                     /EC_number="3.1.3.2"
                     /EC_number="3.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AppA family phytase/histidine-type acid
                     phosphatase"
                     /protein_id="HAY8564457.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(42659..>42885)
                     /locus_tag="JNZ05_001150"
     CDS             complement(42659..>42885)
                     /locus_tag="JNZ05_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564458.1"
                     /translation="AVSDAAVVGRSVGTSLLVARFGLNTAKEVSLSMQRLEQAGVNIK
                     GAILNGVIKRASTAYSYGYNYYGYSYSEKE"
BASE COUNT        10706 a        10450 c        11399 g        10330 t
ORIGIN      
        1 ttatgtacat tggtgcgccg ttggcgaaag acagagaaac ccttttcttc actagcgtac
       61 gggcagtccc ttcaacaacg aagcgggaag agggaaatac cctgaagatt gccacacaaa
      121 gcgtcatcaa acttttctgg cgaccaaaag gtttagcgta tcccttaggc gaggctccgg
      181 cgaaactgcg ttgcacttcg tcagctgaca tggttacggt cagtaaccca acaccttatt
      241 tcattaccct gacagacctg aaaataggtg gaaaagtagt taaaaatcaa atgatttccc
      301 cctttgataa ataccaattc tctctgccaa agggggccaa aaatagcagc gtaacgtatc
      361 gaaccatcaa tgactacggg gcggaaacgc cgcaactcaa ctgtaaatcg taagccgtct
      421 tcagttaaga gagcgagatg tatagaactc atcgacaaca cagcctgtta agctctggtg
      481 gagtgccatc gtttattggt gggctggtgg tgtttgtgtc ggcagcgttc aatgcacaag
      541 ctgaaacctg gttcgatcct gcttttttca aagatgatcc ctcaatggtg gccgatttgt
      601 ctcgtttcga aaaaggacaa aaaataacgc caggggttta tcgtgtcgat attgttctga
      661 atcagacaat tgtagatacg cgcaacgtca attttgttga gataacgcca gagaagggga
      721 ttgccgcctg tttgacgact gaaagcctgg atgcaatggg tgttaatact gatgcgtttc
      781 cggcttttaa acaactggac aaacaagcgt gtgtgccatt ggcggagatt attccggatg
      841 ccagcgtaac gtttaatgtg aataaactcc gtctggaaat ttcagtaccg caaatcgcca
      901 tcaaaagtaa tgcccgtggt tatgtccccc ctgaacgttg ggatgaaggg atcaacgcgc
      961 tattactggg atattcgttt agcggggcta acagtattca tagcagcgca gacagtgatt
     1021 ctggcgacag ctattttctg aatttaaaca gtggcgttaa tttaggccca tggagattgc
     1081 gcaacaattc aacatggagt cgcagtagtg gccaaaccgc agaatggaag aatctcagca
     1141 gctatttgca gcgggcggtt attccactaa aaggcgaact gaccgtaggt gatgattata
     1201 ctgcaggcga ttttttcgat agtgtcagct ttcgtggtgt gcagctggcg tcagatgaca
     1261 acatgctgcc agacagcctg aaagggtttg cgcctgtggt gcgtggtatc gccaaaagca
     1321 atgcccagat aacgattaag caaaatggtt acaccattta ccaaacttat gtatcgcctg
     1381 gtgcttttga aattagtgat ctctattcca cgtcgtcgag cggtgatttg ttagttgaaa
     1441 tcaaagaagc ggacggcagc gtcaatagct acagcgtacc gttttccagc gtgccattac
     1501 tccagcgtca ggggcgaatc aaatacgcgg tgacactggc gaaatacaga accaatagta
     1561 atgaacagca ggagagcaaa tttgcccagg ccacgttgca gtggggcgga ccgtggggaa
     1621 cgacatggta tggtggtgga caatatgctg aatattaccg tgccgccatg tttggtctgg
     1681 gatttaacct tggcgatttc ggagcaattt cgttcgatgc gacccaggcg aagagtacgc
     1741 tggcagagca aagcgaacat aaaggtcagt catatcgttt tctgtatgcc aaaacgctca
     1801 accaattggg cactaatttt caattgatgg gctatcgcta ttcgacgtcg ggtttctaca
     1861 ccctttccga caccatgtat aaacatatgg atggctacga atttaatgac ggtgatgatg
     1921 aagatacgcc gatgtggtcg cgttattaca atttgtttta caccaaacgt ggcaaactgc
     1981 aggtcaatat ctcccagcaa ttaggcgagt acggttcgtt ttatttaagt ggtagccagc
     2041 aaacttgctg gcataccgat caacaggatc ggctattaca gtttggctac aacacgcaaa
     2101 ttaaagatct ctcgctgggg gtttcctgga actacagtaa gtcccgtggt caacctgatg
     2161 ctgatcaggt gtttgcacta aatttttccc tgccgctcaa tctgttgctc cccagaagta
     2221 atgatagcta taccaggaaa aaaattacgc ctggatgacc tctaacacca gtatcgataa
     2281 cgaagggcac attacacaaa acctgggttt aacggagaca ctactcgatg acggtaacct
     2341 gagctacagc gtgcaacagg gatataacag cgaggggaaa acggctaatg gtagcgccag
     2401 catggactac aaaggggcgt ttgcagatgc ccgagtgggc tacaactaca gcgataacgg
     2461 cagtcaacaa caactgaact acgctctttc aggcagttta gttgcccatt cacagggcat
     2521 taccctgggg caatcgctgg gggaaactaa cgtgctgatt gcagcaccag gcgcagagaa
     2581 tactcgtgtg gcgaacagca ccgggctgaa aactgactgg cgcggatata ccgttgtccc
     2641 ctatgccact tcttatcggg aaaatcgaat cgcacttgat gcggcatcgt taaaacgtaa
     2701 cgtggatctt gaaaatgcag tagtcaacga ggttcccacc aaaggggcgt tggttctggc
     2761 ggagttcaat gcccatgcgg gagcaagggt attaatgaaa acatcaaagc agggtatacc
     2821 gctgcgtttt ggcgcgatag cgacgctgga cggcatacag actaatagcg gcattattga
     2881 tgatgatggc tcgctctata tgtccggttt gcccgctcag ggagcgataa ctgtacgctg
     2941 gggcgaagct cccgatcaaa tttgtcatat cagttaccag cttaccgaac aacaaattaa
     3001 ctctgcgatt acgcgaatgg atgccatatg cagataatct ttggagaaaa atgcgtgtta
     3061 ttactacgac tattttttgc cgccgtctta atgctatggt gcgctcaaac tgctgcttat
     3121 atcgggcagt gtcataccac tcaggggaat ccgtatattg gcgtcaattt tggcgttaaa
     3181 actctggatg aagaagaaaa tacggctgga gtagttaaag acaaatttta tcagtggaac
     3241 gaatcgagtg attattatgt ttcctgtgat tgcgataaag acaatgtcag aagtggccga
     3301 tgggcattcg ccgcggattc accgttagtc tatttaggcg acaactggta caaaattaat
     3361 gactatcttg ccgccaaagt tttattgcag gttaaaggca gttctcctac tgcggttcct
     3421 ttcgaaaacg tgggcacagg ggcagataca cgatggcata tttgcgatcc cggcggtcaa
     3481 cgtttaggtg gccagggggc tagcggtaat agcggtagct tttccctgaa aatattgcag
     3541 ccgttcgttg gttcggtcgt cattcctcct atggcgctgg cgcgattatt tgaatgctac
     3601 aacatacccg caggtgattc ctgcacgact actggtacac cggttttagt gtattacctg
     3661 tctggtacga tcaattcact tggctcatgt tccgtcaatg ccggagaaac aatcgaggtt
     3721 gatctgggcg acgtatttgc ggctaacttt cgtgttgtag ggcataagcc tcttggggcc
     3781 agaacggcag aacttgcaat tccggtcagg tgtaacacgg gaaacgcagg gttagttaac
     3841 gtcaatctga gtctgacggc aacctcagac cccagctatc cccaggcgat taagacgtca
     3901 cgtcctggcg tgggcgtggt ggtgaccgat agccagaaca acattatttc ccctgctggt
     3961 gggacactac cgctctctat tcctgatgat gcagacagta tcgcgcgaat gaatgtctat
     4021 ccagtcagca cgacaggcgt accaccagaa accgggcgat ttgaagccac ggcaacggtg
     4081 agaataaatt ttgattaacg cgtgaacgta tgaagaaaaa aacgatatat cagtgcgtta
     4141 ttttgttctt tagccttctt aacatccatg tcgggatggc tgggcctgaa caagtcagta
     4201 tgcatattta tgggaatgtg gtcgatcagg gctgtgatgt cgccaccaaa agtgcattac
     4261 aaaatattca tattggtgat tttaatatca gtgattttca ggccgcgaat accgtaagca
     4321 ctgctgctga tttgaatatt gatatcaccg gttgtgccgc tggtattact ggcgcggacg
     4381 tcctttttag cggcgaggct gacacccttg cgccgacact gctcaaacta actgacacag
     4441 gcggaagcgg tggtatggca acggggattg ccgtgcaaat tcttgatgcg caaagtcagc
     4501 aagaaatccc gctcaatcag gtccagcctc ttacgccctt aaaagccggg gataacacac
     4561 tcaaatatca acttcgttat aagtccacaa aggcgggagc aacgggcggt aatgcgacgg
     4621 cggttctcta ttttgatctg atttaccagt gaaggagtga tgttgaaacg gataatctgg
     4681 attctgttct tactgggatt aacggggggc tgtgagctat ttgcccatga tggcacggtc
     4741 aacattagcg gatcgtttcg ccgtaataca tgcgtgctgg cacaggatag caagcaaatc
     4801 aatgtgcagt taggcgatgt ctcgctgaca cgtttttttc atgggaatta tggcccggag
     4861 aaatctttca tcattaattt gcaggattgc ggaacagatg tcagcacggt tgatgtcacc
     4921 ttttcaggaa cccccgacgg agtgcagagc gaaatgctgt ccattgaaag tggtacggat
     4981 gcagccagtg ggctggcaat tgcgattctg gatgatgcga agatattgat tccgcttaac
     5041 caggcgagta aagactacag cctacatagc ggtaaggtgc cattaacttt ttatgcgcaa
     5101 ttgcgacctg tcaatagcga tgtgcagtcg ggtaaagtga atgccagcgc gacatttgta
     5161 cttcattatg actaatacgt ggaacagatt agcgctcttg attttcgccg ttttatcgct
     5221 gctggtggcg ggtgaattgc aggcaggagt ggtggtcggc ggaacgcgat ttatctttcc
     5281 ggcagacaga gaatcgatat ctattttact gactaatacc agtcaggaat cttggcttat
     5341 taacagcaaa atcaatcgcc caacgcgttg ggccgggggg gaagcgtcga cagtgccagc
     5401 actattactg gccgctccgc cacttattct cctgaagccc ggtactacag gcacgttgcg
     5461 cttgctgaga acggaaagcg acatcttgcc tgtggatcgc gaaacgctat ttgagttaag
     5521 cattgccagc gtgccatccg gcaaagttga aaatcagagc gtaaaagtgg cgatgcgctc
     5581 ggtatttaaa ctgttctggc gacacgaagg tttgccgggc gacccgctgg aagcttatca
     5641 acaattacgc tggacacgga attcgcaggg tgtacaactc actaacccaa cgccttacta
     5701 cattaacctg attcaggtga gcgtaaatgg taaagcgtta agtaatgcgg gagtcgtgcc
     5761 gcctaaaagc cagcgtcaga caaactggtg tcaggtaata gcaccgtgtc atgtcgcctg
     5821 gcggacgata aatgattacg gcgggttgag tgcaaagaag gagcaaaatc tgccctgaaa
     5881 caggttctga aaacgtttgc gttttttttg ccgcaggtca attccctttt ggtccggact
     5941 cgcacataat acgcccccgg tttgcacacc gggaatccag gagagttcat gtactacccc
     6001 ttcgttcgta aagccctttt ccagctcgat ccagagcgcg ctcatgagtt tacttttcag
     6061 caattacgcc gcattaccgg aacaccgttt gaagcactgg tgcggcagaa agtgcctgcg
     6121 aaacctgtta actgcatggg cctgacgttt aaaaatccgc ttggtctggc agccggtctt
     6181 gataaagacg gggagtgcat tgatgcgtta ggcgcgatgg gatttggatc gatcgagatc
     6241 ggtaccgtca cgccacgtcc acagcctggt aatgacaagc cgcgtctctt tcgtctggta
     6301 gatgccgaag gtttgatcaa ccgtatgggc tttaataatc ttggcgttga taacctcgta
     6361 gagaacgtaa aaaaggccca ttatgacggc gtcttgggta ttaatatcgg caaaaataaa
     6421 gatacgccag tggagcaggg caaagatgac tatctgattt gtatggaaaa aatctatgcc
     6481 tatgcgggat atatcgccat caatatttca tcgccgaata ccccaggatt acgcacgctg
     6541 caatatggtg aagcgctgga tgatctctta accgcgatta aaaataagca aaatgatttg
     6601 caagcgatgc accataaata tgtgccgatc gcagtgaaga tcgcgccgga tctttctgaa
     6661 gacgaattga tccaggttgc cgatagttta gttcgccata atattgatgg cgttattgca
     6721 accaatacca ctctcgatcg ttctctggtt cagggaatga aaaattgcga tcaaaccggt
     6781 ggcttaagtg gtcgtccgct tcagttaaaa agcacagaaa ttattcgccg cttgtcacta
     6841 gaattaaacg gtcgcttacc gatcatcggt gttggcggca tcgactcggt tatcgctgcg
     6901 cgtgaaaaga ttgctgcggg tgcctcactg gtgcaaattt attctggttt tatttttaaa
     6961 ggtccgccgc tgattaaaga aatcgttacc catatctaat tatttcttcg aattcctctt
     7021 tataagataa tcagggcttt attttcagcc ctggttgttt tatattcatc gctgttgctt
     7081 atttagacat tttgtacttt tattgttgag gttattaagc gaagcaacaa tggattgtgt
     7141 tgctgcggtt ttatagggta ggggagaggc agatgcgaat taaaccagac gataactggc
     7201 gttggtatta cgatgaagag cacgatcgaa tgatgctcga tttagccaat ggtatgctat
     7261 ttcgctcacg ttttgcgcgc aaaatgttga ctccagatgc cttttctccc gcaggctttt
     7321 gcgttgatga tgccgcgctc tatttctctt ttgaagaaaa gtgccgcgat tttaatttat
     7381 ctaaagacca aaaagccgaa ctggtgctga atgcactggt agcaattcgt tatctcaaac
     7441 cgcaaatgcc gaaaagctgg cattttgtct cccatggtga aatgtgggtg cccatgccgg
     7501 gcgatgcggc ctgcgtttgg ttgagcgata ctcacgagca ggtcaatttg ctggtcgttg
     7561 aatctggcga aaatgccgcg ctatgcctgc tggcacaacc ctgcgttgtt attgcgggtc
     7621 gcgcgatgca gttgggcgac gccattaaaa tcatgaacga caggctgaaa ccgcaggtga
     7681 atgttgacag cttcagcctc gaacaggcag tctaacgcgt cagcttaagg gcagtcttcg
     7741 gtacacagct acagcaaaga atggtgccat catcgcccat tgctgatttt ttcagcggcg
     7801 tgacttcgcc ttctaaaagc tgaacacggc aacttccaca aatgcccgcg cggcaagaat
     7861 aagggatacg aattccctga ttttctaatt gctccagcaa cacttgttgg ttatttccac
     7921 gaaatgcctg tccctgccag tcaatatcta catttgcgtc cggttgttgc gtgatgttgg
     7981 cggtatcatc agcggcagct gcgccgtaaa tttttgccgg agccgttgcc agaatttcca
     8041 cctcatcgcc aacgcgaatc acgccgctat tacgggcaat taaattctga ccaaaatcga
     8101 catcgccgtt atcctgggca gtgcggaaag attgcaatgt ttttaatggt tcgcctgccg
     8161 gatgtttttg ccctttttct gggctgacgg tggtgaaaat acagcggcta caaggtttaa
     8221 ccacatcaaa caccacatca ccaatgcgaa tcactttcca gctatcttct tcccaggctg
     8281 acgcgccgga aaccaccaga ttggggcgga attgctccat ttttacactg gccggacaac
     8341 gttgttggag atcacgtaac gaggcttcgt tagcaagaag gtaaggatag ccatcagcaa
     8401 atgacagagg tacagtgttg tggcgtttca cgcgccgggt catttgtggc cccacccagc
     8461 gtaattgcac ttcgcgggag aaaaatccac ttagccattt gttgatcgcg tctggcgcaa
     8521 ttcgcgcggt aaaatgtgtg ccccaaactt cggttggtgc gtcttgtgtg gcgaaatcag
     8581 caaaacgaac atatgcgcta ctgccatctg gtgcggtgag atgcaagcca tcatgcacag
     8641 gtgaaggagt aaaccgtacc atctggggaa actggcgagc ggtaataaac gtaccgtcag
     8701 gttccgtgat cataaagatg cgatcgaagg ccagaccact gacatcagcc agagcatgtg
     8761 taagaccaat gccgcgcatc gatttaacag gatgaataaa aagccgggtt aatgtcgcca
     8821 cggtgcggtc ctcaaatgaa aataagccct caactttatg acatgacgcg cttattagct
     8881 ataatgcgca acaattttct tagcgcctgt cccgttaggc tattttattt gtcagtttgg
     8941 ccctgggttg tgctcgaaat cctcacgtac ttgtgtacgc tccggtttct ccgcgctctc
     9001 catgtccaga ctgactgcaa caattacgcc tactgcggta ggctcttaga gtaaaagtga
     9061 cgatatgaat tctctgtttg ccagtacggc ccgtgggctg gaagagctgt taaaaactga
     9121 actggaaaat ctgggggccg ttgaatgcca ggtggttcag ggtggggtcc atttcaaggg
     9181 cgacacacgg cttgtttacc agagcctgat gtggagccgc ctggcctcgc gtattatgtt
     9241 gccgctgggc gagtgtaagg tttacagcga tttagacctc tatctcggtg ttcaggcgat
     9301 caactggaca gagatgttta atcctagcgc gaccttcgct gtccacttca gtggtttgaa
     9361 tgacaccatc cgcaacagtc agtacggcgc gatgaaagtg aaagacgcga tcgtcgatgc
     9421 tttcacgcgg aaaaatctgc cgcgtccaaa tgttgatcgc gatgcgccgg atatccgcgt
     9481 taacgtctgg ctgcataaag aaaccgccag tatcgctctt gatctgagtg gtgatggttt
     9541 acatctgcgt ggctatcgcg atcgtgctgg tattgcgccg atcaaagaaa ccctggcagc
     9601 cgctattgtg atgcgatccg gctggcagtc aggaacaccg ctgctcgatc cgatgtgtgg
     9661 ttccggtacg ttgctgattg aagcggcgat gctggcgacc gatcgcgcac caggcttgca
     9721 ccgtgggcgt tggggcttta gcggctgggc gcagcatgat gaagctatct ggcaggaagt
     9781 gaaagcggaa gcgcaaactc gcgcccgtaa aggcctggct gagtatagct ctcattttta
     9841 cggttcggac agcgacgcac gggtgattca acgtgcgcgc actaacgccc gtcttgcggg
     9901 gattggtgaa ctgatcacct ttgaggtgaa agatgtcgcg caactgacca atccgctgcc
     9961 gaaagggccg tacggtacag tgttgagcaa cccgccatac ggtgaacgtc tggacagcga
    10021 accggcgctg attgcgctgc atagtctgct gggccgaatc atgaaaaacc agttcggtgg
    10081 ctggaatctc tctttgttta gtgcctcgcc ggatctgcta agctgcctgc agctgcgtgc
    10141 agacaaacag tacaaggcga aaaacggccc gctggactgc gtacagaaaa attaccacgt
    10201 tgccgaaagc acaccagaca gtaaaccggc aatggtagcg gaagattacg ccaaccgcct
    10261 gcgtaagaac ctcaaaaaat tcgagaagtg ggcacgccag gaagggattg aatgttaccg
    10321 cctgtatgac gccgatctgc cggaatataa cgttgccgtt gaccgttatg ccgactgggt
    10381 ggtggtgcag gagtatgcgc cgccaaaaac tattgatgcg cacaaagcgc gtcagcgtct
    10441 gttcgatatt atcgctgcaa ccatttcggt actggggatt gcgccaaaca aactggtgct
    10501 gaaaacccgt gaacgccaga agggcaaaaa ccagtaccag aaactgggcg agaagggcga
    10561 gtttcttgaa gttaccgaat ataacgctca cttgtgggtg aacctgacgg attatctcga
    10621 tactggtctg ttcctcgatc accgtatcgc ccgccgtatg ctcggtcaga tgagcaaagg
    10681 caaagatttc ctcaacctgt tttcttatac cggcagcgcc accgtgcatg cgggattagg
    10741 cggtgcacgc agcaccacca ccgtggatat gtcgcgtact tatctggaat gggcagaacg
    10801 caacctgcgt ctgaatggcc tgactaggcg tgcgcatcgc ctgattcagg ccgattgcct
    10861 ggcgtggctg cgtgaggcaa atgaacagtt cgatctgatc tttatcgatc cgccaacctt
    10921 ctctaactca aaacgaatgg aagatgcgtt tgatgttcag cgcgatcatc tggcgctgat
    10981 gaaagatttg aaacgtctgc tgcgtgcagg tgggacgatc atgttctcga acaacaaacg
    11041 tggcttccgt atggatctcg acggcctggc aaaactggga ctgaaagcac aagaaattac
    11101 gcaaaaaacg ctctcccagg atttcgcccg taaccgccag atccacaact gctggctgat
    11161 taccgcagcc tgaaaggaat agtaatgtca ttaatcagta tgcatggcgc atggctgtcg
    11221 ttcagcgacg cgccgcttct cgataacgca gaactgcata tcgaagataa cgaacgtgtt
    11281 tgtctggtgg gccgcaacgg cgcaggaaaa tcgacgttaa tgaaaatcct caaccgtgaa
    11341 caagggctgg atgacggtcg cattatttat gagcaagatt tgattgtagc tcgtctgcaa
    11401 caggatccgc cgcgtaacgt tgaggatagc gtttatgatt tcgttgccga aggcattgaa
    11461 gaacaagcgg aatatctgaa acgctatcac gatatttcgt gcctggtgat gaacgacccg
    11521 agcgagaaaa atctcaacga actggcgaag gttcaggaac agctggatca ccacaacctg
    11581 tggcagctgg aaaaccgcat caacgaagtg ctggcgcaac tggggttaga tcctaacgtt
    11641 gcgctgtcgt cgctttccgg cggctggttg cgtaaagcgg cattaggacg cgcgctggtg
    11701 agcaatccgc gcgtattgct gctggacgaa ccgactaacc atctggatat cgaaaccatc
    11761 gactggctgg aggggttcct taaaaccttc aatgggacga ttattttcat ctcccacgac
    11821 cgttcgttta tccgcaatat ggcgacgcgc attgttgatc tcgatcgcgg caagctggtg
    11881 acctatccag ggaattacga ccagtacctg caggaaaaag aagaagccct gcgcgtggaa
    11941 gaactacaaa atgccgagtt cgatctcaaa ctggcgcagg aagaagtgtg gattcgctag
    12001 gggatcaaag cacgccgtac ccgtaatgaa ggccgcgtac gcgccctgaa agcgatgcgt
    12061 cgcgaacgtg gtgaacgtcg cgaagtgatg ggtaccgcaa agatgcaggt ggaagaggcc
    12121 agccgctccg gtaagatcgt tttcgaaatg gaagacgttt gctaccaggt taacggtaag
    12181 caactggtga aagatttttc tgcccaggtt ctacgtggcg acaaaattgc cctgattggt
    12241 ccgaatgggt gcggcaaaac cacgctgctg aaactgatgc tcggtcagct tcaagcgggc
    12301 agcgggcgta ttcacgttgg caccaaactg gaagtggctt atttcgatca gcaccgcgcg
    12361 gaactggatc ccgataaaac ggtgatggat aaccttgccg aaggtaagca agaggtgatg
    12421 gttaatggca agccacgcca cgtattgggc tatttgcagg actttctgtt ccatccgaaa
    12481 cgggcgatga cgccagtacg tgcgctttct ggcggtgagc ggaaccgctt gctgctggcg
    12541 cgtttgttcc tcaaaccaag caacttattg attcttgacg aaccgaccaa cgatcttgat
    12601 gtcgaaacgc tggaactgct ggaagaactg atcgacagct atcagggcac ggtattgctg
    12661 gttagccacg atcgtcagtt tgtcgataac accgttacag aatgctggat cttcgaaggc
    12721 ggcggtaaaa ttggtcgtta tgttggcggt tatcatgatg cccgtggtca gcaagaacaa
    12781 tatgtggcac tcaaacagcc tgcggtgaaa aaaagcgaag aagccgccgc gccaaaagca
    12841 gaaactgtaa aacgcagcag tagcaaacta agctataaat tgcagcgcga actggagcag
    12901 ctaccgcaat tgctcgaaga tctggaggcg aagctggaag ccctacagac gcaagtggcg
    12961 gatgcttcct tcttcagtca gccgcatgag cagacgcaaa aagtgcttgc tgatatggct
    13021 gctgcagagc aggagctgga gcaagccttt gaacgctggg agtatcttga agcgttaaaa
    13081 aatggtggct gatcgcaaaa tagtcgattt aaacagagac agctgacaac ggtaaaattg
    13141 cctgatgaac tgggtttatc aggcatgaaa accaacactt tgttagtcat ctgtaaaata
    13201 gcgcatcatt aaggagtacc aatgtgcgaa catcatcatg ccgcgaagca catcctgtgc
    13261 tcgcagtgtg acatgctggt ggcgttaccg cgccttgagc atggtcagaa agcggcatgt
    13321 ccccggtgtg gcacaacgtt aaccgtggcg tgggatgccc ctcggcagcg tcccaccgcc
    13381 tatgcgttgg ctgcactgtt catgctgttg ctgtccaact tgtttccttt tgtgaatatg
    13441 aacgttgcag gagttaccag tgaaattaca ttactggaaa ttcccggcgt gcttttttct
    13501 gaggactacg ccagcctcgg cacctttttc ctgttgtttg tgcaactggt tcccgcgttt
    13561 tgtctgataa ccattctgtt actggtgaat cgcgcggaat taccggtccg tttaaaagag
    13621 caactggcac gggtgctttt tcaactcaaa acctggggaa tggcggagat tttcctcgcg
    13681 ggtgtgctgg tcagtttcgt taaactgatg gcttacggca gcattggggt aggcagcagc
    13741 tttctcccct ggtgtttatt ttgtgtcctg caactgcgcg cttttcagtg cgttgatcgt
    13801 cgctggttat gggacgacat cgccccgatg ccagaactgc gccagctgct aaaaccaggc
    13861 gtcacgggga tacgtcaggg gctgcgttcg tgctcctgtt gtacggcaat ccttcctgct
    13921 gatgaacccg tgtgcccgcg ttgtagtacc aaagggtacg ttcggcgtag aaacagcctg
    13981 cagtggacac tcgcgctgct tgtaacgtcc atcatgctgt atcttccggc taatattttg
    14041 cccatcatgg tgacggattt attaggctcg aagatgccgt cgacgattct cgctggggtc
    14101 attctgttat ggagcgaagg ctcttatccc gtcgctgcgg tgatctttct ggccagtatt
    14161 atggtgccaa cgttaaagat gatcgccatc gcgtggctgt gttgggatgc caaagggcat
    14221 ggcaagcgcg acagtgaaag aatgcatttg atttatgaag ttgttgagtt tgtaggccgc
    14281 tggtcgatga ttgacgtttt cgttatcgcg gtgctctcgg cgctggtgcg tatgggaggt
    14341 ttaatgagta tttatccggc aatgggtgca ttaatgtttg ctttagtcgt cataatgaca
    14401 atgttttctg ctatgacgtt tgacccgcgt ttgtcgtggg atcgtcaacc tgaatcagag
    14461 catgaggagt cctgacagta tggaatctaa taatggggaa gccaaaatcc agaaagtgaa
    14521 gaactggtct cccgtgtgga tatttcctat cgtcacggcg ctcattgggg cctgggttct
    14581 tttttatcat tacagccatc agggaccgga agtgaccctg atcaccgcga atgcggaagg
    14641 aattgaaggt ggcaaaacca ccattaaaag ccgtagcgtt gacgtcggcg tggttgaaag
    14701 cgccacactg gctgatgatt tgacgcacgt tgaaatcaaa gcgcggctga attccggtat
    14761 ggaaaaattg ctgcataaag acaccgtctt ttgggtggtg aaaccgcaga ttggtcgcga
    14821 agggattagc ggcctgggaa cgctgctgtc tggagtttat atcgaactgc agccaggcgc
    14881 gaaaggcagc aaaatggata aatacgattt gctggactca ccaccgttgg ccccgcctga
    14941 tgcgaaaggt atccgtgtga ttctcgatag caaaaaagcc gggcagctct cgccaggaga
    15001 tccggtgctg ttccgtggct atcgggtagg ttcggttgaa accagcacct tcgatacgca
    15061 aaaacgcaat atcagttatc aactgttcat caatgcacct tatgaccgac tggtgaccag
    15121 caatgttcgc ttctggaaag atagtggcat tgcggttgat ctgacgtcag cggggatgcg
    15181 tgtggagatg ggctcattga caacgctgct tagtggcggt gttagctttg atgtgccgga
    15241 aggtctggat ttagggcagc cagtggcacc gaaaacagct ttcgttttgt atgatgatca
    15301 gaagagcatt caggattcgt tgtacaccga tcacattgat tatctgatgt tctttaaaga
    15361 ttcggtacgc ggtctgcaac cgggagctcc ggtagaattc cggggtattc gcctgggtac
    15421 cgtaagcaaa gtgccattct ttgcgccgaa tatgcgtcag acatttaacg atgattaccg
    15481 tattccggta ctgattcgta tcgagccaga gcggctgaaa atgcagcttg gcgaaaatgc
    15541 ggatgttgtt gagcaccttg gcgaattgtt gaaacgtggt ttacgcggat cgctgaaaac
    15601 cggaaacctg gtcactggtg cactgtatgt tgatctcgat ttctatccaa atacgcctgc
    15661 aataaccggt attcgtgaat ttaatggtta tcagattatc ccgactgtta gcggcggcct
    15721 ggcgcaaatc cagcaacgac tgatggaagc gttggataag atcaacaaac tgccattgaa
    15781 tccgatgatt gaacaggcaa ccagtacgct ttctgaaagt cagcgcacaa tgaaaaacct
    15841 gcaaacgacg ctggatagca tgaacaagat cctcgccagc cagtcgatgc agcagttgcc
    15901 gacggatatg cagtcaacgt tgcgtgaatt gaatcgcagc atgcagggct tccagcctgg
    15961 ctccgcagcc tacaacaaga tggtggcgga tatgcagcgc cttgatcagg tgttgcgaga
    16021 actgcaaccg gtgctgaaaa cgctcaatga gaagagtaac gcgctggtat ttgaagcgaa
    16081 ggacaaaaaa gatccagagc cgaagagggc gaaacaatga aaaagtggct agtgacgatt
    16141 gcagcactgt ggctggccgg atgcagctcc ggcgaaatta ataaaaacta ttaccagtta
    16201 cctgtggtgc agagcggtac acaaagtacc gccagccagg gcaatcgtct gttatgggta
    16261 gagcaggtca ctgttcctga ctatctggcg gggaatggtg tggtttatca aaccagtgat
    16321 gtgaagtatg tgattgccaa caacaacttg tgggccagcc cgttggatca acagttgcgc
    16381 aacaccctgg ttgccaacct gagtacgcaa ctgcccggct gggtggttgc ctcccagcct
    16441 ctgggaagcg cccaggacac gctcaatgtt accgtaacgg agtttaacgg tcgctatgat
    16501 ggcaaggtca ttgtcagtgg tgagtggctg ttgaaccacc agggacaact gatcaaacgt
    16561 ccgttccgtc tggaaggagt gcaaactcag gatggttacg atgagatggt taaagtgctg
    16621 gccggtgtct ggagtcagga agccgcttct attgcacaag agataaagcg tctaccttaa
    16681 ttataaagat ttgtaaatat aaccgtctcc ggtatgttgc ctgaggcggt ttttttgttt
    16741 ctaacgtgcg gaaaaatttg ttcctcttca cattttttgt acaaccgaca tgcccgtgta
    16801 gctcacaaat atgacagtgg cgtgaatttt gcgcattgac ggcagttatg attcgcggta
    16861 ttgcttaact gtgattgcac atttagtaat cactgttttc ttttccacca gaaaccagta
    16921 tgagggaaac gaggcatgaa gagacaaaaa cgagatcgcc tggaacgggc acatcaacgt
    16981 ggttatcagg ccggcatcgc cggacgctca aaagaaatgt gtccctatca gacgctgaat
    17041 caaaggtcac aatggctggg aggctggcga gaagccatgg cggacagggt agtaatggcc
    17101 tgattctgtc tctttaaaaa gaaacctccg cattgcggag gtttcgcctt ttgatactct
    17161 gtctgatgat aatcagaagg cagacgtatc ctggaacaga ccgactttca ggtcgctggc
    17221 ggtatagatc agacgacaat caaccagcac ttcgccatct gccagaccca taatcagacg
    17281 acgattaaca atgcgtttaa agtgaatacg gtaggtcact tttttcgctg tcggcagtac
    17341 ctgaccagtg aatttcactt cgccaacgcc cagcgcgcgg cctttacctt cgccgcccag
    17401 ccagccgagg tagaacccta ccagctgcca cattgcgtcc aggcccaggc atcccggcat
    17461 aaccggatcg ccaatgaagt ggcatccgaa gaaccacaga tccggattga tatccagttc
    17521 tgcttcaaca taccctttgt cgaagttacc acccgtttcg gtcattttga ccacacggtc
    17581 catcatcagc atgttcggtg ctggcaattg cgggccttta gcgccaaaca gttcaccgtg
    17641 accagaggca agaaggtctt cttttgtata ggattcgcgt ttatctacca tgttctctgt
    17701 aagccttatt ttattgaagc acgcaggata gctaacacgt gtacgctgaa caagtccgat
    17761 cagttcggaa taaaccagtt cagccaacgt aatggccatg gaaaacggtg acgtccttcc
    17821 tgttgcgatg cttgcgcgat acgttcctgg atggtttgca tcagcgtcgt ttggccttcg
    17881 ccatcccaca ccagatttaa taataacggc agtgcgtcag tcacatcgtc tactgcccag
    17941 atggtgaatt tgccttcttc taccgctttc accagttcac tgtgaagact taaatggcga
    18001 acgttagcag tggggataat gacaccttgt ttccccgtta actcacgttg ctggcaaata
    18061 gcaaagaagc cttcgatttt ctcatttaaa ccaccgaccg gctggacgcg gccgaactga
    18121 tcgactgaac ctgtgatagc gatactctga ttcactggca catcagcgag ggcgcttatc
    18181 agggcgcaga gttcagccat cgaggcacta tcgccatcca cttcactgta tgactgctca
    18241 aatgtcagcg atgctgagaa ggggatctgt tgctcaagct gtagttccga catcaggaac
    18301 gcctgcatga tcatcatccc tttcgcatgg atattgccgc caagctccgc tttgcgttcg
    18361 atgtcggtga attcaccatc gccaatatgc acaacgcagc taatgcgaga aggttcgcca
    18421 aaagcgcgtg gatgacccgg aaattcaatg accgaaaggg cgttaatttg cccgatgcgt
    18481 tcgccttcgg tttcaatcag gatttgctcc tgaaggatct catcctgcat acgttcagcg
    18541 aggaaacctt cgcgccattc acgctgctgc agcattaagt ttagctgctc gccggagaag
    18601 gtgtcgccat cacacaggga ggcgacctct ttacactggc ggaggatcca atgcgggcta
    18661 agcggaagtg tttcttgttc accggtgtag cgtgctgctt cgcggataag taccggccaa
    18721 gcatccgctc ccggggcagg taagtgatta tgtctggcgg taaatgtcac ccagcgacac
    18781 cactggctta ctgactccgc atcgacaatc tgcagagtat cttcaaattc gctataaata
    18841 gcctgctctg aaagctctgg ctccatctcc tggaaatcag ccaatgattc gcgttcgcct
    18901 accagaatga ctttcagctt caatggcatc gaaggcacag agacggggag agggcgcgac
    18961 tcatcaaacg caacccagtc aaaacgctcg cggttaacga tatttttcag ccgcatccac
    19021 agcagaggtt gcgccagcag tgtacgcaaa gagatgatga gaataccgcc atttgcctga
    19081 tgcaccagac caggctgcag ggtaatgtcg ccattaaact ggcgcaggca gccaaagagt
    19141 tgctccgctt ctacccagtc ggcagcgaca acttgcgtta aagtcgcaaa attatcatct
    19201 gcactcactg cgtgacgtaa gcggatggag tggccggaaa cctcatagtg atcgcccacc
    19261 agttggcctg catcgctttg tagcgtacgc gcggcatcgg caataagatt cagatactca
    19321 gactcttccg gggccttagc cagcataaaa gaggaggatg ctcgcgtatg cagcaactgc
    19381 tccagcgcaa attgcagtcg cggttgagta tcactgaata aaggatcgtt ttcgtcaatc
    19441 aaatgtggct gagcaaatat ttcctgatag ctatcggtat caggaaccaa gtcacgccat
    19501 gcaagtttcg taatggtcaa agttgatgtt ttttagtctg ttgtcaaagc cgcgattata
    19561 ccgtaaccgg cactacagca cacgtagaaa gcaccgacaa tactcctggc tgggcgttaa
    19621 agctcacagg atggagattc ttttcttcac tggcctaaaa agctgatatt ctgtaaagag
    19681 ttacacggta acattgagat cgctatgaaa tatcaacaac ttgaaaatct tgaaagcggt
    19741 tggaaatgga agtatctggt gaagaagcat cgcgaagggg agttaattac ccgttacata
    19801 gaagccagtg ccgcccagga agccgtcgat gtgttgctct cgctcgaaaa tgaacccgtg
    19861 ctggtaaatg gctggattga caagcatatg aatccggaac tggtcaatcg catgaagcag
    19921 accattcggg ccagacggaa gcgccatttt aatgcggaac accagcatac gcgcaaaaaa
    19981 tcgatcgatc tggaatttat tgtctggcaa cgtctggctg gtcttgcaca gcgtcgcggt
    20041 aaaacgcttt ctgaaacgat tgttcagctg attgaagatg cggaaaacaa agagaaatac
    20101 gcgaataaaa tgtcttctct gaagcaggat ctgcaggcat tgctgggtaa ggaataactg
    20161 acgaaagtca gttcaattta ctaaaggcaa aaaaaacccc gcagcagcgg ggtttttcta
    20221 ccagacgata acttaagcct gcggctgagt tacaacgtct ttgatacctt taacttcgat
    20281 ctctacgcga cgatccggag ccaagcagtc gatcagtgca gcacgctgtt tcacgttgtc
    20341 acaggtgttg ccagtaaccg ggttggattc gcccatacca cgtgcggaga tcttgtctgc
    20401 cgggatacct ttggagatca ggtaatcaac aacagactga gcacggcgct cggacagacc
    20461 ctggttgtaa gcgtcagaac cgatgcggtc agtgtaaccc agaacaacta cggaaccgtc
    20521 tttcggatcc aggttgctca gctggctgta cagctgatcc agagcagcct gaccttccgg
    20581 tttcagggtt gctttgttga agttgaacag aacgtcagac ttcagagtga agtgcttggt
    20641 ctgtacttcc ggtgccggag ctggagcaac taccggagct gcttcgccct gaccgaaacg
    20701 gtaggaaaca cccaggctca gcagaccgtt gtccggacga gtaccgatgg tgtttgcgtc
    20761 accgatgttg ttggtccact ggtattccag acgggtagcg atttcaggag tgatcgcgta
    20821 ctcaacacca cctgcgaaaa ccggagaaac gccagtgtcg tggtctttaa aggatgcgcc
    20881 accaggtacg ttagccttgg tgtctgcacg ccataccata ccacccagac gagtgtagat
    20941 gtccagatcg tcagtgattg ggtaacccag tttagcggtc agctgaacgc cctgagcttt
    21001 gtatgcgccg ttgatgttgt cgcctttgta cggcatacga cctaaccagt cgtaacccat
    21061 ttcaaaacca acatacgggt taacctggta accaccaaaa gcacctgcac ccagttggtt
    21121 ttcgtgggtc ggaccattgt taggaataaa accagtgtca tggtactggg accagcccag
    21181 tttagcacca gtgtaccagg tgttatcttt cggagcggcc tgcgctacgg tagcgaaacc
    21241 agccagtgcc actgcaatcg cgatagctgt ctttttcatt ttttgcgcct cgttatcatc
    21301 caaaatacgc catgaatatc tccaacgaga taacacggtt aaatccttca ccgggggatc
    21361 tgctcaatat taactctacc gatatcttcg gcttatgccg agcacccctg gcgatgtaaa
    21421 gtctacaacg tagttgaaaa cttacaagtg tgaactccgt caggcatatg aaaaaaaagt
    21481 cttgtataag gtatgtttaa tctttttgtc agcgacaatt tacagaaaaa aaacgcggaa
    21541 accgcttcag acaagccttc gcaaggaaaa ttagtcacga ctgaaagcat tggctgggcg
    21601 acaaaaaaag ttccaggatt aatcctaaat ttacttaatg atacaaatta gagtgaattt
    21661 ttagcccgga aagttgtctc gttgtgtgag aggatgcgct taccggacgc ataataaacc
    21721 ccatagcgtt accttcattt gccgcatcaa caagttcagc atgctcttct tcagtcaaat
    21781 catctgtcaa ccaaccgatc accacactgt aattgcccgt gcgtaaagcg cgaaccattg
    21841 actccactgt gtggcaaggg gagagctggc taatctgcat tactttcgtt aagggtagcc
    21901 cagatgcctg aacccattcc cgactcagtt tttgttgcgg tgttaaccag agttgccagc
    21961 gcgattgctg accgagttgc tgtaacaatg gcaacagtag aagttgcgtc atcatgggct
    22021 gatcttcgcg atagacaact tcactgataa gcccggctgt agtgttttcc gtagagacac
    22081 gcgcaatttt acttgctgcg gatgagaacg acgaagaacg atgtgcatag cctgaagtgt
    22141 acataatcaa tccagcccct gtgagttact gtatggatgt gcagtacatc cagtgacaac
    22201 aaagatcaac cctattttcg gaaagagcct cgcaaatttt gtcgttggtg acgggaaaac
    22261 ataaattaat cttgcccctt aagaataagt tgcctatttt cgtagttaac ggatccgtta
    22321 atgtgaatca ttcttttatg ttatgatttt aaaaggaatt ttatgaaaag cctctcctat
    22381 aagcggatct ataaatcaca agaatacctg gcaacgttgg gcacaattga ataccgatca
    22441 ttgtttggca gttacagcct gaccgttgac gacacggtgt ttgcgatggt ttctgatggt
    22501 gagttgtatc ttcgggcttg tgagcaaagt gcacagtact gtgtaaaaca tccgcctgtc
    22561 tggctgacat ataaaaagtg tggccgatcc gttaccctca attactatcg ggttgatgaa
    22621 agtctatggc gaaatcaact gaagctggtg cgtctgtcga aatattctct cgatgcagcg
    22681 ctgaaagaga aaagcacgcg caatacccgg gaaagactga aagatttgcc caatatgtct
    22741 tttcatctgg aagcgattct cggggaggtg gggattaagg atgtacgggc gttacgtata
    22801 cttggggcaa aaatgtgttg gttgcgactg cggcagcaaa acagtctggt gacagaaaag
    22861 attctgttta tgcttgaagg tgccattatc ggcattcatg aagctgcgct cccggtggca
    22921 cgccgccagg agcttgcaga atgggctgac tctcttacgc cgaaacagga gtttcctgcg
    22981 gagcttgagt aatctggcgt tgcagacgac caatctctgg cagtaatgca atcaataacc
    23041 caacttgttg tacgaccaga ggttctttgc tatctgcccg tggttcaagt tgctgcatcc
    23101 gctgtttcag gccagctaat gcctgattga cgcgttcttc atcagcaggt tgatgatgta
    23161 acgcgtcatc aacatagcaa actgcgtcat caagaaacgc cagaatttca ggattagtta
    23221 actgctcccg gtgagcaccg agggctgaga tatagctggt aaacgtatgg ttaaggcaca
    23281 gcaagcgaaa cgcggcttcg cgaatttgcg gggtaacgtt cggttcgctg gacatatttg
    23341 ataccaccga cgccagttca gcatcacggt tgtgtgcatc gcggcgggca atacgatacg
    23401 ccagacggtt atcacgcccc tgatggtatt gctccagtat ggcatcgaga tagcgacagt
    23461 tggcctctgt ggcgcgttcg agcattcgcg gcagattgcg aaacttccag tcaggccaga
    23521 tgtagctcac tgccgcccac gcaatggcac aaccaatcag cgtatcgatt acgcgaggta
    23581 acgctacttc aaaaccttca cccagtaagt taaaacacag tagcaccaaa agtgtgatga
    23641 acatcgttgc atgagcgtat tgcacgttac ggaaggcaaa aaagagcacg ccggtaataa
    23701 ccagcagcac cagctgccct tccagtgatg gcacaaacca cagcacagga atgccaatcg
    23761 cgatacctac cagcgtacca ataatcctta acttcaggcg gtggcgcgtg gcgttatagt
    23821 ttggctggca gacaaacaaa cttgtcagca agatccaata cccgtgatgc attccggtta
    23881 tttgaatgat ggcgtagccg aagcacaaca ccagcgacat tcttaccgca tgacggaaga
    23941 gggcggattc cggcgtgaag tgacggctaa gacgcagcca gatatcactc aatccgtgcg
    24001 ggctgtcatc agcgagctca ttttcgtcat tattacgggg tagtgcctgg gcctgttctg
    24061 attcaattgt tgccagttgg gcatcaatgg cgcgtaaatt gttcagcaaa aatcccagtg
    24121 ttttgagtaa atcggcgggt gcgccgttat cgcgtatccg ctccagcgca gcatcaatat
    24181 gcgtaaaagc gcgctcaaaa tgcggatcat gttgataaga ctgacgcaac aaaatacagc
    24241 gtgacagttg ctggcacgcc tggccctgca tcgacatcag ccgctgaaaa cggaacagca
    24301 cgtcgctgtg gcgaaaatgt tcacgcaatg tttgatactg aatatgagaa gagctggcac
    24361 gctcgtgaat atcctgtgcg acaaagtaat aatgcagcgt gcgacgcgtt ccccgttgac
    24421 cacgatcgcc acgtaagcgg gtcagcagcg agagtttcgt ctgattcaat gtcgccatca
    24481 gctgaccgtt ggcgagagcc aaatcgtaca gcggtgcctg gctttcatct tcaatatcag
    24541 gatcaaacat gcgcgacttg agctcaagat aacgcgccag ttgttcatag caacgcgcca
    24601 ggttgtcctg cagcgggcgg accgggaaca gcagatgacc aataagtgtc aggacgttgt
    24661 accagacggc accggccagc agatacatcg gctgctgata ccagtgctca tacagtgatg
    24721 ttcccaacat agtgtaaatg gcgatcagca atgcaccgaa ggcaattgtt gcatagcgtt
    24781 gacccagacc gccgagcaaa atgaagccgc tggtagagag cgttaagcca atcgcaaata
    24841 gccagggcca gggaaacagc aattctactg aggccgaggc gataaaaaag cagaacagcg
    24901 taatgatgag gttacgcaaa cgtcccgcca gtcggtcatc gagatcggtc agcgccgctg
    24961 ccaccatccc cagcgttagc ggaatcgtca gttttacatc acccagccac cacggaaacg
    25021 ctgtggttcc acaaagcgca ataaaaatac gcgcgtaata cagccaggcg ctgttccaag
    25081 tatagcgttt gagcaaagga cttagcataa aggccatatt agtggattat tcaaaacgac
    25141 gacgtgcatt ggcttcacgc gcagcttgcg ctgtttctac cgataccacg cgacgaccaa
    25201 ccggccatag tgcaatagcg gcaattttaa agttcgcaat gccgacagga atgccaatga
    25261 ttgaaataca ttgtgcgatg cccgttgcaa tgtgcatcag gcataaccac cagccaaaga
    25321 aaatcagcca gaaaatattc aataccgtac cgccagtatt cagcaacaca tttttgccag
    25381 ccgggttcag ttcatcgaca tgaatagctt cattgccata aggcaccaga gacagtttag
    25441 tgatctccca gcaggatcgt gtcagcggta aggtaaaaat cagcacaata ctgaccagag
    25501 tcgccaacag ccagcccaga gtggtggcaa atccgccaag cacaaagttc agaatgttca
    25561 aaacggtacg cataaaacct cgctttactg tggttttcag taatgggcgg caattgtaac
    25621 gtttttttgg gctggagcac gttttctctg acggttacac tgataagaaa taatttcgtg
    25681 tggatctaca gagtcatgga actgaaagcg acaacgcttg gaaaacgtct ggtacagcac
    25741 ccttacgatc gggcggtgat cctcaatgcc gggattaaag tctccggcga tcgccacgaa
    25801 taccttattc ctttcaatca attactggcg attcactgta agcgcggtct ggtatggggc
    25861 gagctggaat ttgtactgcc ggatgaaaaa gtggtgcgtc tgcacggcac agaatggggc
    25921 gagacgcagc gtttttacca tcatcttgat gctcactggc ggcggtggag tggcgagatg
    25981 agcgaaattg cgtctggtgt tttacgccag caactggatt tgattgccac gcgcactgga
    26041 gaaaataaat ggctgacgcg tgagcaaacc tctggcgttc agcaacaaat ccgccaggct
    26101 ttgtcggcgt tgccgttgcc ggttaaccga ctggaagaat tcgataactg ccgtgaggcg
    26161 tggcgtaaat gtcaggcttg gttgaaagat attgaaagcg ctcggttgca gcataaccag
    26221 gcgtataccg aagccatgct taccgagtat gcggattttt tccgccaggt cgagtcttca
    26281 ccgctgaatc cggcgcaggc ccgggcagtc gttaatggcg agcattctct gttagtgctg
    26341 gccggtgcag gaagcggaaa aacgtcggtg ctggtggccc gtgcaggctg gttgctggcg
    26401 cgtggtgaag cgtcccctga gcaaatttta ttgctggcgt ttggtcgcaa agccgctgaa
    26461 gagatggacg agcggattcg cgaacggcta cataccgaag acattaccgc acgcacgttt
    26521 catgcgctgg cgctgcatat tattcagcag ggcagcaaaa aagttccgat agtcagcaaa
    26581 ctggaaaatg ataccgctgc ccgtcatgaa ctctttattg ctgagtggcg caagcaatgc
    26641 agcgaaaaga aagcgcaggc gaagggctgg cggcaatggc tgacggaaga aatgcagtgg
    26701 tcagtgccag aaggtaactt ctgggatgat gaaaaattac agcgtcgcct tgcctctcgc
    26761 ctcgatcgct gggtaagtct gatgcggatg cacggtggtg cacaggcaga aatgattgcc
    26821 agtgcacccg aagagattcg cgatctgttc agtaaacgta tcaagttgat ggccccgtta
    26881 ttaaaagcct ggaaaggtgc gctgaaggca gaaaacgctg tcgatttttc gggccttatt
    26941 catcaggcga ttgtgattct ggagaaaggc cgctttatca gcccgtggaa gcatattctg
    27001 gttgatgaat ttcaggatat ctcgccgcag cgcgcagcgt tgttagcggc attacgcaag
    27061 caaaacagtc agacgacgtt gttcgccgtt ggtgatgact ggcaggcgat ttaccgattc
    27121 agcggtgcgc aaatgtcgct caccaccgct ttccatgaaa actttggtga aggcgatcgc
    27181 tgtgatttag acacgactta ccgttttaac agtcgtatcg gtgaggtggc aaaccggttt
    27241 attcagcaga acccaggcca gctgaaaaag ccgctaaaca gcttaaccaa tggagacaaa
    27301 aaagctgtca cgttattgga tgagagtcaa cttgacgctt tgctggataa gctctctggt
    27361 tatgccaaac cggaagagcg cattctgatc ctggcgcgtt accatcacat gaggcctgcc
    27421 agcctggaaa aagcggcaac acgctggccg aagttgcaaa tcgactttat gaccattcat
    27481 gccagcaaag ggcaacaggc ggattacgtc atcatcgttg gcttgcagga ggggagtgat
    27541 ggttttccgg ctgcggcgcg ggagtcgatt atggaagagg cgctactgcc accggttgag
    27601 gatttcccgg acgctgaaga acggcgttta atgtacgtgg cgctgacccg ggcacgccat
    27661 cgggtatggg cactgtttaa caaagagaat ccctctccct ttgtggaaat actgaaaaat
    27721 ctggatgtgc cggtggcgag aaaaccgtaa gaaacaggtg gcgtttgcca cctgtgcaat
    27781 attacttcag acggtcagcg agataacgct gataatcggg gataagaata tcgaccgcgt
    27841 cgttgaaatg cggcgactgg ataatgaagt ctgccgttgc cacgttggtg gcgaccggaa
    27901 tgttccatac cgtcgccaga cgcagcaagg ctttcacgtc aggatcgtgc ggcacggcat
    27961 ttagtggatc ccagaagaaa atcaatacat caattttccc ttctgagatc aatgcgccaa
    28021 cctgctggtc accccccatt gggccactca acatcgcgtt gacgttcatg ccggtcgcgc
    28081 gggaaatcaa gttaccggta gtgcctgttg catacagtac gtgttgttcc agtaacggtt
    28141 gatgccgttc cacccagctc atcagcattt gtttgcagtg atcgtgtgcc accagcgcaa
    28201 tatgtttccg cgcaggtaaa gtgcgagtcg tcagttccat aatgtacatc cgtagttaac
    28261 tttcctacag attactgtaa gcacttatcg ctgcaagata aagaccgaaa aagcctgcgc
    28321 acaggcacaa aaatctcagg aagatggttg tttttccgcc cattgcagga aagtatttcg
    28381 cgtttgtggg tcagccagtt taaaccaata cttcagccgt tgttctgtga gcacctgaga
    28441 ctgcggtgga atagcatcca gctcggaaac acccgaaagc agcgtactgt catctgccat
    28501 catcgtggca aattgcggta gtgaagcgcg tttcgcggat ttgttatagc gttcaacctc
    28561 gacctcataa tcaatcgttt ttgccgttga ggtaatggcg agaatatcca gctttaccgg
    28621 aatcggcgtc gcatcgccat ccaacaattc aaggcgcggc gcggcatcaa aatggttagc
    28681 ttcccgctca ttctccaggc gaggcaggcg aaaattgacc tggttgatga gctgggtatt
    28741 aaaactgacc accagcggtg gggagatata cagccgttct tcactattgg aaagatgaat
    28801 cgtcttctca acgcgaaaca ctaactgatg cggtccattg tccagttcaa tgctgtccgc
    28861 accgcgcagc agagagctgg aaaccttttt gccgtcgagt accagaagat cgacatcggt
    28921 tgaaagccgc agcgtggtgg caaaaacgga taccggcaaa cataatgcaa tcaaggtggt
    28981 cacgatgccg gttttcatag gtagctcctg tcaaaagacc gcccgaataa taatgtgtca
    29041 aaatttttct ttaatcatac ttactaacat atagacatat ttcccgcttt tgctctcatt
    29101 cattcgtatt agctgcatgg ttggcatgtc ggctttgtcg tacactttgt taaacagtct
    29161 ggaggaaata atgaaagaaa ccgatattgc aggcatttta acgtccactc acaccattgc
    29221 gctggtgggg gcaagcgaca aacccgatcg ccccagctat cgcgtgatga aatatctact
    29281 cgaccagggg tatcacgtca ttccggtttc gccaaaagtt gccggcaaaa cgctgctggg
    29341 ccagcagggg tatggcacgc tggcagacgt tccggaaaag gtcgatatgg tcgatgtttt
    29401 ccgtaattca gaagcggcgt ggggcgtggc acaggaagct attgccattg gcgcgaaaac
    29461 cctctggatg caactggggg tgattaatga gcaagcggca gtactggcac gggatgccgg
    29521 gttaaatgtg gtgatggatc gctgcccggc tattgagatc cctcgcctgg gtctggccaa
    29581 ataaaaaatc cccggaaggc aaaaaccttc cggggatttg ttcagggatt agttacgcag
    29641 acgcggggcc tggagttgtt tgcggatggt ctgcgccagc tcatccatgg atggctgttc
    29701 aggatgttca tcctgcaatt cgctacttag ctgggcttca gccaggtagg tatgaaccgg
    29761 taggccgtta tcgtcctcca tcaccacgtg ataccacgga gcggcgcgaa gctcgtcatt
    29821 caccgccagc tcatcaggcg acggttcaga gagcgaataa accgggtcga tatccacgac
    29881 cactccgaga taacctaaca gggaatggcg gacctgctgg ccgataccga atttgctggc
    29941 aatcatagtc acctcccggg aaatctttac ctttgatata agggtaaaat tccacatttc
    30001 aagttacatg acgcgacagg caaacccttt cagatatagc ccttccgggt aggtagcgat
    30061 caccggatga tcggctgcct gacggaactg ctctataaat tgtacatcac gaccggcatc
    30121 aattgcggca tccgcgatga ttttctgaaa taaatcgctg gtcatcagac cggaacagga
    30181 gaaagtcagg agaataccgc cttcattcag caactgaatc gccagcatgt tgatgtcttt
    30241 ataaccacga cacgcgccca tcaactggct tttattctca acaaacttcg gcgggtccat
    30301 cacgataacg tcaaattttt caccgcgatc gcgataagta cgcagcaatt taaagacatc
    30361 atcacggaca aactcagcct tgctcagatc cagtttgttc agctcaacgt tctgccgtgc
    30421 aatatccagc gcttcctggg aggtatcaac gctgacaacc tggctgcaac cgcccatcag
    30481 tgccgatacg gcgaaaccac cggtatagga gaaacagttc agcacacgtt tattttcaac
    30541 gtagcggcgg gtagccaggc ggctgtctcg ctggtccaga tagtagcccg ttttgtgtcc
    30601 gtgctgaata tccaccagca gcttcatgcc gtgttcttca atcggcagca gggcaggcgg
    30661 caactcgccg gtgacgaggc cctgggtcag ctccatccct tcttttttac gtaccgcaac
    30721 atcgctgcga tcgtaaatcg cacattccgg gtacagcgtt tgcagggcac taattaatgc
    30781 cgcgcgctga tattctgcgc cagcactcag cagttgcagc accagaaaat taccgaaacg
    30841 atcgatagta atacccggca ggccatcaga ttctccggcg attaaacgat agctgtcgag
    30901 gccatctttt tgcgccagcc agtcacgcca tttttgtgct tgttgcaaac ggcgggaaaa
    30961 aaaagcaatg tcgatagact cagacgggtc aaacgtccag acgcgcgccc ggatttgcga
    31021 agctggcgaa taagcgccgc gtgctaacca ttttccctga tgatcaacaa tatcgatggt
    31081 ttcaccgagg ctggctttac cttccatgcg ggcaacggcc ccggaaaaga cccacggatg
    31141 gcgacgaagt aatgattttt cgcgcccttt ggctaacact aaacgtacac tcataattta
    31201 cttttctgtc gatgcaaaga aatgggcgtc attgtccgga gttttccatg gatttgcaac
    31261 gcgtccatta aggataagga gagcagcaat gtcgaaagtc tgcataattg cctggattta
    31321 cgggcgagtt cagggcgtag gatttcgcta caccacacag tacgaagcga aaagactggg
    31381 cttaaccggg tacgccaaaa atcttgacga tggcagcgtg gaagtggttg cctgcggtga
    31441 agaagggcag gtagaaaaat taatgcagtg gttaaagagt ggcggcccgc gttctgcgcg
    31501 tgtagaacgg gtgctcagcg agccgcatca tccctcgggg gaattaaccg attttcgtat
    31561 ccgttaaata cattttaccg gttttggcag gccagcaatt ttggtggctt gcttagccgg
    31621 gcctttcggg aacagtcggt ataaatagcg gctgttacct ttttcttcac caaatttatt
    31681 cgccatcgct tttaccagca tacgaatcgc cggagacgta ttaaattcca ggtagaagtc
    31741 acgtacaaaa cgcaccactt cccagtgttc tggcgacagc gaaatccctt cgttctctgc
    31801 aatcaccact gccagcggtt cactccactg gctgctttct ttgagatagc cttcggtatc
    31861 catttctatc tctttacctt cgaagatcag cataattatt actaccttaa tcagactggc
    31921 ggcagtgtaa caaaaaacaa agccccgcat aaagcgaggc tatgaaagtg ttagcgggtg
    31981 agattaatcg cggctggcga agcccagaat gctcagcagg ctgacgaaga tgttgtacag
    32041 cgaaacatac aggctaaccg tggcacgaat atagttcgtt tcaccgccat gaatgatgtt
    32101 gctggtttca aacaagatag cgccagagga gatcagaatg aagaccgcgc tgatcgccag
    32161 atgcagagca ggcagttgca ggaagatatt agcaaccata ccaatcagta ccaccacaat
    32221 acccgccatc agcataccgc cgaggaacga catatctttg cgggtggtca gcacatatgc
    32281 agagcagcag aagaacacta acgccgttcc gcccagtgcc atagcgatta cgtcacccat
    32341 tccggcagac agataggtgt tcagaattgg tccgaggata taacccagaa aaccggtaaa
    32401 ggcgaatgcg gagataatcc cggtcggctt attcgccgtt ttataggtca ggaacatcaa
    32461 accatacata cccaccagcg tcagaatcag acccggagat ggcagcatca gcaccgtact
    32521 ggcagttgcg gtaatcgccg aaaaggccag cgtcaggctc agcagaaaat aggtattacg
    32581 cagcacctta tgggtgctaa gcagtgatgt acggtcatgt gaagaactaa caatacgatc
    32641 cattgagtca ctctctatga cagatgtaat taattaagca gcataatgat aatgcgtaag
    32701 ggcacccaga agttttaccc atctttacgc atttgatctg gaacaggttt aacagcggat
    32761 tatcaggtca ttaagcaaat ataacgccct gagaattccg acaggcaaaa gaaaaagggg
    32821 ttagcattta gctaacccct tatcttattt ggcggaagcg cagagattcg aactctggaa
    32881 ccctttcggg tcgccggttt tcaagaccgg tgccttcaac cgctcggcca cacttccgga
    32941 atgacgcgca ctataaacat cccgatgcgg cgtgtaaacc cctaatttgt ttgtttgcct
    33001 gaaaaacagc caaaagtgca ttgatagcgt gaaataacag cagattgatc atttcatcac
    33061 catgaattcc ttctctttta ctcgtttagc aaccggctaa acatccccat cgcccggcta
    33121 aagaaaaata ggtccatttt tatcgcaaaa agataaatcc acacagtttg tgttgttttg
    33181 tgcaaaagtt tcactacgct ttattaacaa tactttctgg cgacgtgtgc cagtgcagaa
    33241 ggatgagctt tcgttttcag catctcacgt gaagcgatgg tttgccttgc tacagggacg
    33301 tcgcttgccg accataagcg cccggtgtcc tgccggtgtt gctaggagaa gagacgtgcg
    33361 atatgaataa cgaggaaaca ttttaccagg ccatgcggcg tcagggcgtt acccggcgca
    33421 gctttctcaa atattgtagt ctggctgcca cgtcgctggg attaggcgcg ggaatggcac
    33481 caaagattgc ctgggcgctg gagaacaaac cgcgcattcc ggtggtatgg atccacggtc
    33541 tggaatgtac ctgctgtacc gaatctttta tccgctccgc tcacccactg gcgaaggacg
    33601 tcatcctttc cctgatttcc ctctattacg acgataccct gatggctgcc gccggaaccc
    33661 aggcggaaga agtctttgaa gacatcatca cgcaatataa tggcaaatat atcctcgcag
    33721 tagaaggtaa tccgccgctg ggcgagcagg ggatgttctg tatcagcagc ggtcgaccgt
    33781 ttattgagaa actcaaacgt gccgctgccg gagccagcgc gattatcgcc tggggaacct
    33841 gcgcgtcctg gggctgcgtg caggccgcgc gacctaatcc gacacaggca acgcctatcg
    33901 acaaagtcat caccgacaaa cccattatca aagtacctag ctgcccgccg atcccggatg
    33961 tgatgagcgc catcattact tacatggtga cctttgatcg cttgccagat gtcgacagaa
    34021 tgggtcgtcc actgatgttt tatggtcagc gtatccacga taaatgctat cgccgcgctc
    34081 acttcgacgc cggagagttc gtccagagtt gggatgatga cgctgcccgc aaaggttatt
    34141 gcctgtacaa aatgggctgc aaagggccta ccacctataa cgcctgttcc tccacacgct
    34201 ggaatgatgg cgtttctttc ccaatccagt ctggtcacgg ctgcctgggc tgtgcggaaa
    34261 atggtttctg ggatcgcggt tcgttctaca gccgcgtggt cgatattccg caaatgggta
    34321 ctcattccac cgccgatacc gtcggtttaa ccgcgcttgg cgtggtggca gcagctgttg
    34381 gtgtgcacgc agttgccagc gccgttgacc aacgcagacg tcataaccag caacctacag
    34441 aaaccgaaca tcagccaggc aatgaggata aacaggcatg agcactcagt acgaaactca
    34501 gggatacacc atcaataatg ccggacgccg cctggtggtc gacccgatta cgcgcatcga
    34561 aggccacatg cgctgcgaag tgaatattaa cgatcagaat gtgatcacca atgccgtctc
    34621 ctgcggcacc atgtttcgcg ggctggagat catcctgcaa gggcgcgacc cgcgcgatgc
    34681 gtgggcgttc gttgaacgta tctgcggcgt ctgtactggc gtacacgccc tggcttcggt
    34741 ttacgccatc gaagatgcca tcggtattaa agtgccggac aacgccaata tcatccgcaa
    34801 cattatgctg acaacgctct ggtgccacga tcatctggtg cacttctatc agcttgccgg
    34861 gatggactgg atcgacgtgt tagatgcgct gaaagccgac ccgcgaaaaa cctccgaact
    34921 ggcgcaaagt ctctcctctt ggccgaaatc atcccctagc tatttcttcg acgtacaaaa
    34981 tcgcctgaag aaattcgttg aaggcgggca gttggggatc ttccgcaatg gctactgggg
    35041 gcacccgcag tacaaattgc cgccagaagc caacctgatg ggctttgccc actatctcga
    35101 agctctcgat ttccaacgtg aaattgtcaa aattcacgcg gtctttggcg gtaaaaaccc
    35161 gcatccaaac tggattgtcg gcgggatgcc ttgcgccatt aacattgacg aaagcggcgc
    35221 ggtcggggcg gtcaatatgg aacgcctgaa cctggtgcag tcgattatca cccgcacggc
    35281 agacttcatt aacaacgtga tgatccccga cgccttagcc atcggtcagt tcaacaaacc
    35341 gtggagcgaa atcggcacgg gtctttctga taaatgtgtc ctcagctacg gcgcattccc
    35401 ggatattgcc aacgactttg gagagaaaag tctgctgatg cctggcggcg cggtgattaa
    35461 cggcgacttc aacaatgtgc tgccagtgga tttggttgat ccgcagcagg tgcaggagtt
    35521 tgtcgatcac gcctggtatc gttatcccaa cgatcaggtc gggcgtcatc cattcgatgg
    35581 cattaccgac ccgtggtaca accccggcga tgtcaaaggc agcgatacca acattcagca
    35641 gctgaatgaa caggaacgct actcgtggat caaagcgcca cgctggcgcg gtaacgcgat
    35701 ggaagtgggg ccgctggcac gcacgttaat cgcttatcac aaaggcgatg ctgcgaccgt
    35761 tgagtcggtc gatcgcatga tgtcggcgtt gaacctgccg ctttccggta tccagtcaac
    35821 gttgggccgc aggtaagttg cagtatttct tcgacaagct gatgaccaac ctgaaaaatg
    35881 gcaatctcgc cactgcttcc actgaaaaat gggaacctgc aacctggccg acagagtgcc
    35941 gtggcgtcgg ctttaccgaa gcgccgcgcg gagcgttagg ccactgggcc gccattcgcg
    36001 atggcaagat tgatctctac cagtgcgtgg tgccgaccac ctggaacgcc agcccgcgcg
    36061 atcccaaagg gcagattggc gcttatgaag cggcgctgat gaacaccaaa atggcgatcc
    36121 ccgagcaacc gctggagatc ctgcgtactc tgcacagctt tgatccgtgc ctcgcctgtt
    36181 caactcatgt gctgggcgac gacggtagcg agctgatctc cgtgcaggtg cgttaacagc
    36241 gaaggagaat catcatgcaa cagaaaagcg acaacgttgt cagccactat gtctttgaag
    36301 caccagttcg tatctggcac tggctgacgg tgttatgcat ggcggtgttg atggtcaccg
    36361 gttacacaaa tttggcaaaa taagtaacaa ggagcgttca tgagcgagca acgcgtggtg
    36421 gtcatggggc tgggcaacct gctgtgggcc gatgaaggct tcggcgtgcg ggtggcggaa
    36481 cggctgtatg cccattacca ctggcccgag tatgtggaga ttgtcgatgg cggtactcag
    36541 ggactgaact tgctggggta tgtcgaaagc gccagccatc tgttgattct cgatgccatt
    36601 gactacgggc tggaacctgg aaggcttcga acctatgccg gagaacgcat tccggcctat
    36661 ctcagcgcga agaaaatgag cctgcatcag aacagtttct ccgaagtgct ggcgctggca
    36721 gatatccgcg gacatctgcc agcacatatt gccctcgtcg ggctgcaacc cgcgatgctc
    36781 gacgactacg gcggcagcct gagcgaactg gcacgggagc aactgcccgc tgcggaacaa
    36841 tcggcgctgg cgcagttggc tgcgtgggga agtgtgccgc aaccggctaa tgaatcgcgt
    36901 tgtctcaatt atgactgtct gtcgatggaa aattacgaag gcgttcgctt gcgccagtac
    36961 cggatgacac aggaggagca gggatgagca acgacacgcc atttgatgcg ttgtggcaac
    37021 gaatgctggc gcgcggctgg acgccggtca gtgaatcccg tcttgacgac tggcttacgc
    37081 aagcgccaga cggcgtggtg ttattaagca gtgacccgaa acgcacgcca gaggtcagcg
    37141 ataatccggt aatgattggc gaattactgc acgagtttcc cgactataca tggcaggtgg
    37201 cgattgctga ccttgagcag agcgaagcca tcggcgatcg ctttggcgcc tttcgctttc
    37261 ctgccacttt agtgtttacc ggcggaaact atcgcggcgt gctgaatggt attcacccgt
    37321 gggcggaact gataaacctg atgcgcgggc ttgtcgaacc gcagcaggag cgtgcctcat
    37381 gagcgaaact tttttccatc tgctggggcc aggaacgcaa ccgaacgatg acagtttcag
    37441 catgaatcca ctgccgatca cctgtcaggt gaatgatgaa ccgagtatgg cggccttgga
    37501 gcaatgtgct cacagcccgc aggtgattgc gctgttaaac gagttacaac atcaactaag
    37561 cgaacgccag ccgccgttgg gcgaggtgct ggcagtcgat ctgttaaatc tcaacgccga
    37621 cgatcgtcac tttatcaata cgcttctcgg ggaaggggaa gtgtcagtgc gcattcagca
    37681 ggctgacgac agtaaaagtg aaatacagga ggcgatcttc tgcggattat ggcgggtgcg
    37741 cagacgtcgc ggcgaaaagt tgctggagga caaactggag gctggctgcg cgccgctggc
    37801 gttgtggcag gcggcaacgc aaaatctctt gccgacagat tcgctgttac cgccgcccat
    37861 tgatggcctg atgaatggcc taccgttggc gcatgagtta ctggcacatg tacgtaaccc
    37921 cgacgcgcag ccgcacagca ttaatctgac gcaattaccc atcagcgagg ctgatcggct
    37981 ttttctctca cgtctctgtg ggccgggaaa tattcagatt cgtaccattg gctatggcga
    38041 gagctatatc aactccacgg ggttacgcca tgtctggcat ttacgctgta cggacacctt
    38101 aaaaggcccg ttactggaaa gttatgaaat ctgcccaata ccggaagtgg tgctggcagc
    38161 gccagaagat ttggtcgact ctgcgcagcg gcttagcgag gtatgtcagt ggctggcgga
    38221 agctgcaccg acgtaaaaag acggtaagta tcgctttcag tcttatgaat atcgcaatcg
    38281 gcgaatacct ctggtcgtag agtttcagga taaagaggga gatctaacat tatcgggtta
    38341 tttttctctc ttcgcctaca ggagtgtgca tgtgggatgt cattgattta tcgcgctggc
    38401 agtttgctct gaccgcgctg tatcactttt tatttgtacc ccttaccctg gggctgattt
    38461 ttttgctggc tatcatggaa accatttacg tggtcaccgg caaaacaatc taccgcgata
    38521 tgacgcgctt ctggggtaag ctcttcggta tcaattttgc tcttggcgtg gctaccggcc
    38581 tgaccatgga gtttcagttt ggtactaact ggtcattcta ttccaactat gtgggcgata
    38641 tttttggcgc accactggcg atggaagcat taatggcctt cttcctcgaa tccacctttg
    38701 tcgggctgtt cttcttcggc tggcaacggc tgaataaata ccagcacctg ctggtgacgt
    38761 ggctggtggc gttcggttca aatctctctg cattgtggat attgaatgcc aacggttgga
    38821 tgcaataccc gaccggtgcg cattttgata tcgacaccct gcgtatggag atgaccagct
    38881 tcagcgagct ggtctttaac ccggtcagcc aggtgaaatt tgtgcacacc gtcatggcgg
    38941 gctacgtgac cggggccatg tttattatgg cgatcagcgc ctggtattta ctgcgcggac
    39001 gggagcgcga tgtcgcatta cgctcgtttg ccatcggttc cgtcttcggt actctggcga
    39061 ttatcggtac cctgcaactc ggagacagtt ctgcgtatga agtcgcgcaa gtacaaccgg
    39121 taaaactggc ggcgatggaa ggggagtggc aaacggaacc tgctcctgca ccgttccatg
    39181 tggttgcctg gccggaacag gatcaagagc gtaacgcctt tgccatcaaa attcccgcgc
    39241 tgctagggat cctcgccact cactcattag ataaacccgt gccgggtctg aagaatttga
    39301 tggctgaaac ctacccacgc ttgcaacgcg gacgtatggc ctggctgtta atgcaggaaa
    39361 tatctcaagg caatcgtgag ccgcatgtgt tgcaggcatt ccgggagctg gaaggcgacc
    39421 ttggttacgg catgttgctc tcccgctatg cgccggatat gaatcatgtc acagccgcac
    39481 agtaccaggc ggcgatgcgt ggcgcgatac ctcaggttgc gccggtattc tggagtttcc
    39541 gcatcatggt gggctgtggt tccctgctgc tactggtgat gctgattgcg cttgtccaga
    39601 cgctgcgtgg caaaatcgac cagcatcgct gggtgctgaa aatggcgctc tggagtttgc
    39661 cgttgccgtg gattgcgatt gaagccgggt ggtttatgac cgagtttggt cgtcagccgt
    39721 gggcgataca ggacatctta ccgacatact ccgcgcactc cgctttaacc acaggacaac
    39781 tggctttctc actgatcatg atcgtaggtc tttacaccct gttcttaatc gccgaagtct
    39841 acctgatgca gaaatatgcc cgtctggggc cgagcgcgat gcagagtgaa caaccgacgc
    39901 agcaacaggg gtaaaggaga aaatcatgtt tgattatgaa acattgcgct tcatctggtg
    39961 gctgctgatt ggcgtgatcc tggtggtctt tatgatctcc gacggatttg acatggggat
    40021 cggctgtttg ctgccgctgg tggcgcgtaa tgatgatgaa cgccggatag tgattaatag
    40081 cgttggtgcg cactgggaag gcaatcaggt ctggttgatc ctcgcaggtg gggcattatt
    40141 tgccgcctgg cccagagtgt atgcagcggc gttttccggc ttttatgtgg cgatgatcct
    40201 ggtgctgtgc tcgctgttct tccgtccgct ggcctttgat tatcgcggaa aaattgccga
    40261 tgcacgctgg cgtaaaatgt gggatgccgg tctggtcatc ggcagtctgg tgccgccggt
    40321 agtcttcggt atcgccttcg gcaacttgtt gctcggcgtg ccgtttgcct tcacaccgca
    40381 attacgcgtg gagtatctcg gcagcttctg gcaactgctg acgccattcc ctttattgtg
    40441 tggtttgctc agccttggga tggtgatttt gcaaggtggc gtctggttac aactgaaaac
    40501 tgttggtgtg attcatctgc gttcacagct ggcgactaaa cgcgctgcac tgttggtgat
    40561 gctgtgcttt ttgctggcag gttactggct gtgggtcggt attgatggct ttgtactgct
    40621 cgcccaggat gctaacggtc cttccaatcc gttaatgaaa ctggtggcag tgctacctgg
    40681 tgcctggatg aataatttcg tcgaatcgcc cgttttgtgg atcttcccgc tgctggggtt
    40741 cttctgccca ttgctgacgg tgatggcgat ttatcgtggt cgcccgggtt ggggattttt
    40801 gatggcatca ttgatgcaat ttggcgtgat tttcacggca ggcatcacgc tgttcccctt
    40861 tgtcatgccg tcaagtgtga gtccaatctc cagcctgacg ttgtgggaca gcacttccag
    40921 tcagctgacg ctgagcatta tgttggtaat cgtgctgata tttttgccca ttgtgttgct
    40981 ctacactctc tggagctact acaaaatgtg gggacgcatg acaacagaaa ctctccgccg
    41041 taacgaaaac gagttgtact aaggagcagg aacatgtggt atttactttg gttcgtcggc
    41101 attttgttga tgtgttcgct ctccaccctt gtgttggtat ggctggacct gcgtctgaaa
    41161 agttaacgaa cgtaggcctg atgcggcgca ttagcatcgc atcaggcaat caataatgtt
    41221 agatatgaaa agcggaaaca tatcgatgaa agcgatctta atcccatttt tatctcttct
    41281 gattccgtta accccgcaat ctgcattcgc tcagagtgag ccggagctga agctggaaag
    41341 tatggtgatt gtcagtcgtc atggtgtgcg tgctccaacc aaggccacgc aactgatgca
    41401 ggatgtcacc ccagacgcat ggccaacttg gccggtaaaa ctgggttggc tgacaccgcg
    41461 cggtggtgag ctaatcgcct atctcggaca ttaccaacgc cagcgtctgg tagccgacgg
    41521 attgctggcg aaaaagggct gcccgcagtc tgctcaggtc gcgattattg ctgatgtcga
    41581 cgagcgtacc cgtaaaacag gcgaagcctt cgccgccggg ctggcacctg attgtgcaat
    41641 aaccgtacat acccaggcag atacgtccag tcccgatccg ttatttaatc ctctaaaaac
    41701 tggcgtttgc caactggata acgcgaacgt gactgacgcg atcctttgca gggcaggagg
    41761 gtcaattgct gactttaccg ggcatcggca aacggcgttt cgcgaactgg aacgggtgct
    41821 taatttcccg caatcaaact tgtgccttaa ccgtgagaaa caggacgaaa gctgttcatt
    41881 aacgcaggca ttaccatcgg aactcaaggt gagcgccgac aatgtctcat taaccggtgc
    41941 ggtaagcctc gcatcaatgc tgacggagat atttctcctg caacaggcac aaggaatgcc
    42001 ggagccgggg tggggaagga tcaccgattc acaccagtgg aacaccttgc taagtttgca
    42061 taacgcgcaa ttttatttac tacaacgcac gccggaggtt gcccgcagtc gcgccacccc
    42121 gttattggat ttgatcatgg cagcgttgac gccccatcca ccgcaaaaac aggcgtatgg
    42181 tgtgacatta cccacttcag tgctgtttat tgccggacac gatactaatc tggcaaatct
    42241 cggcggcgca ctggagctca actggacgct tccaggtcag ccggataaca cgccgccagg
    42301 tggtgaactg gtgtttgaac gctggcgtcg gctaagcgat aacagccagt ggattcaggt
    42361 ttcgctggtc ttccagactt tacagcagat gcgtgataaa acgccgctgt cattaaatac
    42421 gccgcccgga gaggtgaaac tgaccctggc aggatgtgaa gagcgaaatg cgcagggcat
    42481 gtgttcgttg gcaggtttta cgcaaatcgt gaatgaagca cgcataccgg cgtgcagttt
    42541 gtaatgcata aaaaagagca ttcagttacc tgaatgctct gaggctgatg acaaacgaag
    42601 aactgtctaa tgcgtagacc ggaaaaggcg ttcacgccgc atccggtcac tttcagtttt
    42661 actctttctc ggagtaacta taaccgtaat agttatagcc gtaactgtaa gcggtgctgg
    42721 cgcgtttaat cacaccattg aggattgcgc ctttaatatt aacgccagcc tgttccagac
    42781 gctgcattga caagctcacc tctttggcgg tgttcaagcc aaaacgcgca accagcaggc
    42841 tggtgccgac agagcgtccc acgaccgcag catcgctcac cgcca
//
LOCUS       DADMEQ010000020        40611 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.020, whole genome shotgun sequence.
ACCESSION   DADMEQ010000020 DADMEQ010000000
VERSION     DADMEQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.020"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            231..1664
                     /locus_tag="JNZ05_001151"
     CDS             231..1664
                     /locus_tag="JNZ05_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAY8564459.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="JNZ05_001152"
     CDS             complement(1672..2730)
                     /locus_tag="JNZ05_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY8564460.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="JNZ05_001153"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="JNZ05_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="JNZ05_001154"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="JNZ05_001154"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HAY8564461.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JNZ05_001155"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JNZ05_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HAY8564462.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JNZ05_001156"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JNZ05_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HAY8564463.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="JNZ05_001157"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="JNZ05_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HAY8564464.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="JNZ05_001158"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="JNZ05_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HAY8564465.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="JNZ05_001159"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="JNZ05_001159"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HAY8564466.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="JNZ05_001160"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="JNZ05_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HAY8564467.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="JNZ05_001161"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="JNZ05_001161"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HAY8564468.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIHYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRNILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="JNZ05_001162"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="JNZ05_001162"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HAY8564469.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="JNZ05_001163"
     CDS             complement(17699..18322)
                     /locus_tag="JNZ05_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HAY8564470.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="JNZ05_001164"
     CDS             18619..18894
                     /locus_tag="JNZ05_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564471.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="JNZ05_001165"
     CDS             complement(18971..19213)
                     /locus_tag="JNZ05_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HAY8564472.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="JNZ05_001166"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="JNZ05_001166"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HAY8564473.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="JNZ05_001167"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="JNZ05_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HAY8564474.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="JNZ05_001168"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="JNZ05_001168"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HAY8564475.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGETVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22592..24289
                     /locus_tag="JNZ05_001169"
     CDS             22592..24289
                     /locus_tag="JNZ05_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAY8564476.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            24304..25038
                     /locus_tag="JNZ05_001170"
     CDS             24304..25038
                     /locus_tag="JNZ05_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY8564477.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            25051..25908
                     /locus_tag="JNZ05_001171"
     CDS             25051..25908
                     /locus_tag="JNZ05_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8564478.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(25911..28412)
                     /gene="ptsP"
                     /locus_tag="JNZ05_001172"
     CDS             complement(25911..28412)
                     /gene="ptsP"
                     /locus_tag="JNZ05_001172"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY8564479.1"
                     /translation="MLTIQFLCPLPMPNGLHARPARELKEQCSQWQSEITFINHRQNA
                     KADAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAEL
                     TAHPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHS
                     LATLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNM
                     EQVCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTP
                     SQFLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQ
                     CGVLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEA
                     PGAFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGD
                     KSISYLNVPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLD
                     QILWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSND
                     MTQYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLP
                     LLLGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPE
                     EDVRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTG
                     VGFGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVF
                     SQLARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28437..29474)
                     /gene="ypdE"
                     /locus_tag="JNZ05_001173"
     CDS             complement(28437..29474)
                     /gene="ypdE"
                     /locus_tag="JNZ05_001173"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAY8564480.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKILGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29474..30557)
                     /gene="ypdF"
                     /locus_tag="JNZ05_001174"
                     /pseudo
     CDS             complement(29474..30557)
                     /gene="ypdF"
                     /locus_tag="JNZ05_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30572..31819)
                     /locus_tag="JNZ05_001175"
     CDS             complement(30572..31819)
                     /locus_tag="JNZ05_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAY8564481.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGENGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31841..32167)
                     /locus_tag="JNZ05_001176"
     CDS             complement(31841..32167)
                     /locus_tag="JNZ05_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HAY8564482.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32386..33351)
                     /gene="glk"
                     /locus_tag="JNZ05_001177"
     CDS             complement(32386..33351)
                     /gene="glk"
                     /locus_tag="JNZ05_001177"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HAY8564483.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33555..34811
                     /locus_tag="JNZ05_001178"
     CDS             33555..34811
                     /locus_tag="JNZ05_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HAY8564484.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAIMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34926..35252
                     /locus_tag="JNZ05_001179"
     CDS             34926..35252
                     /locus_tag="JNZ05_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY8564485.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35299..36537)
                     /gene="mntH"
                     /locus_tag="JNZ05_001180"
     CDS             complement(35299..36537)
                     /gene="mntH"
                     /locus_tag="JNZ05_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+) uptake NRAMP transporter MntH"
                     /protein_id="HAY8564486.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36873..38075
                     /gene="nupC"
                     /locus_tag="JNZ05_001181"
     CDS             36873..38075
                     /gene="nupC"
                     /locus_tag="JNZ05_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HAY8564487.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38125..40314)
                     /locus_tag="JNZ05_001182"
                     /pseudo
     CDS             complement(38125..40314)
                     /locus_tag="JNZ05_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40522..40597)
                     /locus_tag="JNZ05_001183"
     tRNA            complement(40522..40597)
                     /locus_tag="JNZ05_001183"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40562..40564),aa:Ala,seq:ggc)
BASE COUNT        10804 a        10106 c         9404 g        10297 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaactag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 gcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccaa
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat gcggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtcccgaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactacccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aaacgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagcagc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggtag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccctc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatattgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aatgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata ccccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ccgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attatttcat cgctacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattgattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aataagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgag atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatgattt ttatggcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggtgtgatg cccacggtaa aatacagtgc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg gcgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgcggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccaaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag cgtttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtctca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaattacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttc aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgcgt cggtattctc ggcggcatgt tgtgtgagac
    23041 gttgaccatg attctggtaa ttgtctgggc accaactacc gcgttgggga tcgatatcgt
    23101 ctctaaaatc ggcattccaa tgatcctcgg tagcgtctgt atcggcttta ttgtgcttct
    23161 ggtgcaaagc gttgagggcg aaaaagaggc cagcgccgcg cggcaggcca agctggcgct
    23221 ggatatcgcc aacaaaacgc taccgctgtt tcgccatgtc aatagcgagt cattacgcaa
    23281 ggtctgcgaa attatccgcg atgacattca cgccgatgcg gtggcgatta ctaataccga
    23341 tcatgtgctg gcctatgttg gcgtgggtga acataactat cagaatggcg atgacttcat
    23401 tagcccgact acccgtcagg cgatgaatta cggaaaaatc atcattaaaa acaatgatga
    23461 agcccaccgc acaccagaga ttcattccat gctggtgatc ccattgtggg agaaaggggt
    23521 cgtgaccgga acgctgaaaa tttactactg ccacgcgcat cagatcacct cgtcattaca
    23581 ggaaatggcg gtcggtctgt cgcaaatcat ctccacgcaa ctggaggttt cacgcgccga
    23641 gcagctacgt gaaatggcaa ataaggcaga gcttcgcgcc ctgcaaagca aaattaatcc
    23701 ccattttctg tttaacgctc tgaacgctat ttcatcgtca atccgtctga atccggatac
    23761 cgctcgccag ttgatcttta atctgtcgcg ttatctgcgc tataacattg aattaaaaga
    23821 cgatgagcaa atcgatatca aaaaagagct gtatcaaatt aaagactata ttgccattga
    23881 gcaggcccgc tttggcgaca agctgacggt tatctatgat attgatgaag aggtgaattg
    23941 ctgcattccc agcctgctga tccagccgtt ggtggagaac gccattgtcc acggtattca
    24001 gccttgcaaa ggtaaaggcg ttgtcaccat cagcgttgca gagtgcggaa atcgggtacg
    24061 cattgcggtg cgagataccg ggcacggcat cgatccaaag gtgattgagc gggtcgaagc
    24121 gaatgaaatg ccgggcaata aaattggcct gctgaatgtc catcatcgcg tgaagttatt
    24181 gtatggcgag gggctgcata tccgccgcct ggagccgggg acggaaattg cgttttacat
    24241 tcctaaccaa cgcaccccag tcgcctcaca ggctacgtta ttgctttgag ccggagtgat
    24301 attgtgaaag tcatcattgt tgaagacgaa ttcctggcac aacaggaact gagctggcta
    24361 attaaagagc acagccagat ggagattgtc ggcacctttg acgacggtct ggacgtgttg
    24421 aagtttttgc agcataaccg cgtcgacgcc atttttctgg atatcaatat tccgtcgctg
    24481 gatggcgtgt tgctggcgca aaacatcagc cagttcgccc ataaaccgtt tattgtgttc
    24541 atcaccgcgt ggaaagaaca tgcggtagaa gcgtttgaac tggaggcgtt tgactacatt
    24601 ctcaaaccgt atcaggagtc acgtattacc gggatgctgc aaaaactgga agcggcctgg
    24661 caacaacagc agaccagcag tacgcctgcc gcgacggtaa cgcgtgagaa tgacaccatt
    24721 aatctggtga aagatgagcg aataatcgtc acgccaatta acgatatcta ttacgccgaa
    24781 gcgcacgaga aaatgacctt tgtctatacg cggcgtgaat cctacgtaat gccgatgaac
    24841 attaccgaat tttgcagcaa actgccgccg tcgcattttt tccgctgcca tcgctcattt
    24901 tgtgtcaatc tgaacaaaat acgcgaaatc gaaccgtggt ttaataacac ctacattctg
    24961 cgactgaaag atctggattt tgaagtgccg gtcagccgca gcaaagtgaa agaatttcgc
    25021 cagttaatgc atctttaatc gggaactttc atgaaagcac caggcttgcc cgccgatcaa
    25081 caatttttcg ccgatctgtt cagcggccta gtgcttaacc cgcaactact ggggcgcgtc
    25141 tggtttgcca gccagcctgc ctcgttaccg gtgggcagtt tatgtattga ttttccccgt
    25201 ctggatatcg tgctgcgcgg cgaatacggc aacctgctgg aagcaaagca gcagcgaatg
    25261 gtggaaggag aaatgctgtt tattccggca cgcgcggcta atttaccgat caacaacaaa
    25321 ccggtgatgc tgttaagcct ggtgttcgca ccgacctggc ttgggttatc gttttacgat
    25381 agccgcacca cgtcgttgtt gcatcctgct cgccagatcc agcttcccag cctgcaacgc
    25441 ggtgaaggtg aagcgatgct taccgccctc acccatctta gccgttcgcc gctggagcag
    25501 aatatcattc agccactggt gttaagtttg ctgcatcttt gccgcaacgt ggtgaatatg
    25561 ccgccgggta attcgcaacc gcgcggcgat tttctctatc acagcatttg taactgggtt
    25621 caggataatt atgcccagcc gctcacccgc gagagcgtgg cgcagttttt taatatcacg
    25681 cccaatcatc tgtcaaaact gtttgctcag catggaacga tgcgttttat cgagtatgtg
    25741 cgttgggtgc gaatggcgaa ggcgaggatg attttgcaga aatatcatct gtcaattcat
    25801 gaagtggcac agcgttgcgg ttttccggat agcgactatt tttgtcgcgt tttccggcgt
    25861 cagtttggtc tgacgccggg agagtacagc gcccgttttc agggctaacg tcagaaggtt
    25921 aattctgttt ccagcagcgt caggatactt tgtgcatctt gcgcggcaaa caacgactgg
    25981 cggaagtttt tattcaccag tttacgcgcc agctgcgaga agactttcac atgattcatc
    26041 ccttcgttag cacccagcgt cagcatgatc accagttcga cttcgcccat ttctgactgc
    26101 cagtcaaccg gtttcgccag ccgggcaatg ctgatactgg aatgacggat ccactgagat
    26161 ttggtgtgcg ggatcgctac gccaaaacca acgccggtgg taacaatctc ttcccgctgc
    26221 cagacatctt cttccagctc aaacggatgt tcagtgcgcc cgttaacgcc gaggttgccg
    26281 cacaggaact ggatcgcctg ctctttattg ctaaaatcct gatcaacaaa gatattctcc
    26341 agcgccagca gtgggcgaac gtcttcttcc ggcgtaaagg cggtgagtaa cgcttcaatt
    26401 tcctgcgcac tgcggcattc acatgcctga cgcgccagtt cccgacacgc ctcgctatcc
    26461 agttgacgaa gctggctttt caccgccgga atacgcgggc tactcatact cagctcatcc
    26521 aggcccagcc caagcagtag cggcagataa cggctttcac cgcccagttc accgcaaatg
    26581 cctacccatt tgccccgctg atgcgcagtg gtaactattt gctgcaacat gcgcaggaac
    26641 gatggcgtaa tcgggttata tagcggcgat acgcgcgggt tattacgatc gaccgcatac
    26701 agatactggg tcatatcgtt ggagccgata ctgaagaaat cgacctcatc gcagaagtgg
    26761 tcgatgatgt agcacaccga cggcacttcc accatgatcc caagcgtaat cgtctctgca
    26821 tgacgcaggc catcgcgctt aagctcaacg atcgcttttt ggatctcgcc tttcacccat
    26881 aagatctgat cgaggctgtg aaccatcggg atcatcaact gggcgttgcc gaaactggcg
    26941 gcgcgcaaaa tggcccgcag ttgagtgcgg aacaggccag caaattccgg gtaaatacgt
    27001 accgcgcgat agccgaggaa tgggttctct tcctggggaa cattcagata agaaatgctt
    27061 ttatcgccgc cgatgtccat cgtgcggaag ataatcggct tgtcgcccgc cgccagtagc
    27121 acctgctggt aggcttcaaa ttgctcctgc tcgtccggcg cgctgtcgcg atccatatag
    27181 agcatttcgg tacggaacag cccgacacct tccgcgccgt tggcaaacgc gcctggcgct
    27241 tccagagcgg tgccgatatt cgccgcaata tcaatacgct tgttatcacg ggaataggcc
    27301 agctgcgcgg cagcctgcgc ctgttgtttt tggcgtttat ccgccagcgt ctgcgcgacc
    27361 tgataataac cgctcaccgc gtcattcggg ttaatcgcca gcacgccgca ctgggcgtca
    27421 agcactgcag gttgcccggc ataacgggca atcgcatcca gcggcaagcc actcagtacc
    27481 gggatcgccg aggcacgggc cagaatcagt gtatgcgagg tgcgcccggt tttctccaga
    27541 atcatacccg caagattttt caaatcaagg ctcaaaaact ggcttggggt taaatcttca
    27601 gccaccagaa tggtcggttt ttccagcacc agattgttgc gcggcttcag ttccggccag
    27661 gtgatatgca gcaactgttc gctgatatcg cgaatgtcgc tgacccgctc gcgcagataa
    27721 tcgctggcag aagcagatag tttggcgcaa acctgctcca tattgctgat gatcgccgcc
    27781 cccagtccct gatgctgttc tgtcatcagg cgacggatat tgcctgcaaa ttcatcatcc
    27841 tgaatcagcg acaaatgagc gctgaggata gttttgcttt cgccgtcacg ctcacgcaat
    27901 tgctgattca gttgctcggc aagcgttgcc aggctgtgct ccagccgggt ggaatcttgc
    27961 gcactggcgg ggattgcccg ataactgtcg aggctgtcgc tttgtaacag ggtcagcgta
    28021 cccacgccga cgccgcttgc cagcacattg ccgtacagta aatccgggtt aaggcggctt
    28081 aatgaacgcg gcagcggatg cgccgtcagt tctgcctgcg tcggctgaac gctgtcgctg
    28141 tcgataaagc gcaccaggat gtactcttcc agcacgcgcc gcgcctgctc ttcatcgctg
    28201 ccgctaatgt tcaggctgca actgtcatta aataaggtgc cagttccaat caacgccagc
    28261 gagcttttag catcggcctt tgcgttctgg cgatggttga taaaggtgat ttcgctttgc
    28321 cactggctgc actgttcttt aagttcccgc gccggacgag catgtagacc gttaggcata
    28381 ggcaaaggac agagaaattg aatcgttaac ataaccgctc cgggttaatc aggagatcat
    28441 ctgaaatccg tcagttgaac aaccgtctca cgcgtaagac gttgaataag ggcagataaa
    28501 agttgctcca tctgcaaaat gtcgcggcaa tcggcaatcg atgccgcgca atgtccgtgg
    28561 cgggtggcag gccccattac caatgtgggt acgccagtac cggttaagtg caccgctcca
    28621 ccgtccgtgc cgccgttact gaacatatcc gcctgcaacg gaacgccaat ttctgccgcc
    28681 acggtttcaa tccacgcggt aagttttggc ggcgcaatca gcgatttatc gcttaacacc
    28741 agcatcggcc cgttaccaat ctgacgatgg ttagccgcgc cataatcaaa gtttttcgcc
    28801 cagcaggcgg tatcaagcac aatggcgacg tccggcgaca ccgcgcgggt agcagtttgc
    28861 ccgccgcgta atcccacctc ttcgctggaa cttgccacca gccacacttc cgcaggcagt
    28921 tcggcgtcgt gcagctcacg tagtaacgtc accagcagat agcaaccgag gcgatcatca
    28981 aaggctttgc ccatcacccg ctggtgaggg aggacctgaa aagtggtatc gaaggtgacg
    29041 cgatcgcctg gacgaattcc cgcctgcatc acttcgtcat aggtgcgcgc gccaatgtcc
    29101 acgcgcatgg cgctaacgtc attcccctgc cggtcgccgt caagcaggcc tagaattttg
    29161 cactcttcac gggtggtgat gcgcaccggc tgcagctggc gggcagccat gcgcacgttg
    29221 ccaaccggca gcacgtcaat agcgccttcg cgggagatgc tgcgcaccat aaatcccact
    29281 tcgtccatat gggcgcagat catcaccttc ggacctgtcg attcattgag gcggatcagc
    29341 accgatccca gaccatcaaa tcgcacttct ttttgcaggc gagccgcttc ttccagcagg
    29401 atctgccgca cttcctgttc cgaggaggcg atcgcatctg cctcgctcaa cgcttttaat
    29461 agcgataaat ccattatgcc tctcccgtga gcaacactgt tttcggcatg gcgtagagca
    29521 cttctgcgcc ttgcggggta accagcacaa catcttcgat gcgcacgccc ccttgcccta
    29581 gcaaataaat ccccggctcc acggtcagca acatgcctgg ctgtagcgtc gtggtgtccc
    29641 gcggtgaaaa acgcggatct tcatggactt caatgccgat agcgtgacca gtgttatgac
    29701 cgaaatagtc gccaaatcca gactcggtaa tgacccggcg cgcggcgtcg tcaatctgct
    29761 ggcagcgcac gccggggcga attgcagaga ttgccgcgag ctgtgcctgc aggacaatct
    29821 gatagacgtc aaacagcggg tgagattcgg cgctcacccc ttcgccattc accagcaagg
    29881 tgcgcgtcat atcagcagta gccctgatac agcgcaccaa aatcgagagt gacaaactcg
    29941 cccgctgcaa caatcttgtc gctggctttg ccgtgcggca gcgccccgcg ccagccgctg
    30001 gcgacaatgg tgtcaaaaga ggctttttct gcaccctgct ggcgcataaa ccactccagt
    30061 tcagcggcta tctcgcgctc gctcatcccc gcctgaataa agcggcgaat atgctccgct
    30121 ccgcgatcgg caatcccaca ggcgaggcgg attatctcca cctcctctgg cgttttgatt
    30181 tgccgcagca catccggcgt ggcgctaacc agtttcgcat tgagttcaga cttccagcgg
    30241 tgcgcggttt cccaactcac ctgctggcct tcaaaaccga gcgtttgcaa ctgctcatcg
    30301 gcaatgattt gattgacgat agtggtaagc gtgttcgtcg cgtcaagcaa atgcagctgg
    30361 tagccttgcg cacgggcttc cacctcaacg aaatagcgcg aatctaccag aatgtgcgca
    30421 ctttcacggc taataaccac atagcctgat ccggtggaga tccccagatg cggctgtttg
    30481 ttctgccgtg aggagagaag caccgcatcc agttgttgca ccttaagcca gttgcgcagc
    30541 gaagcgagta atgtcatctg gattttcctt cttacaggct atcgattaac aatttgcctt
    30601 tgcggaacat catcagacgc aggaacacca ccatcagcgc agtaatgatt gcccccagcg
    30661 cgatccccgc catataaacg cccaggttag tgaccagcgg ccatgcccag atagcggatt
    30721 ccgggaacca ttgcactgcg cccagccaga cagctgcggt tgagccgaca atcgcgccga
    30781 ccatatatga cggaatggcg gtgatggggc tttccagcgc aaacggaatc gcaccctcac
    30841 taatccccat aaaggccagg aacatcgcgg tcttaccctg cggataaagt tgagcgttga
    30901 acaggcgttt gccggttaaa cgacggtcaa taatggtcgc cagacccaga ccaatcggcg
    30961 gaataacgat agcgatagaa cgagcggtga ccggtaatac gtggtcagtg gtaaagctga
    31021 aggcaacgaa acctgcggct ttgttgatcg ggccaccaag gtcgattgct gtcgccgcgg
    31081 cgatccccat tgcgtacatc agcgcgccat tctcacctgc ggcagtcagc acagtacgga
    31141 taccgccgtt gatccagcca ccaaaggggg tgatgacgta gtacatcgcc agcatgacaa
    31201 aaatggcgga aagaatcggt agcaaaaatg tggttttgaa cgctaacagg aaatccggta
    31261 actggatttt ctggttcatc catttcacaa ggtaaccggc aacgatagaa ataatcagcg
    31321 caccaatgaa ggtggacggc accggcgaag aggtcgccca ttgcatagtg ctgggatcga
    31381 agttcagcag ttgcgtcggc tgggtagaca tcaacccgcc gataaaacca gccgggaacg
    31441 ccagtttgcc accgatagag ttcgccacaa atgcagcaaa catcggaatg gcaaaaccaa
    31501 acaacacgcc gccgaaggac tgcgacagcc aggcaaattt aagtaaggag aggtcgaaac
    31561 cggagaattt cccgctatta agagcatcca tgatgccgat ctcagcagga attttaagcc
    31621 agctataagc aatcagctgg ctgaaagcga ggatcacacc gcccataatt aaggtcggca
    31681 ccatgcgtga aatccctgac atcacatgct gcgggagttc accccagaag ctgcttttag
    31741 aggcctgcgg attcttaact gccgctgccg caccggatgc gccaggcaca acggttgcac
    31801 tgcgtttttt aatggccata acaattatcc ctgtcgaata ttactgttgt tcagaggcaa
    31861 tcatctcttc gatttctttg atgatgcccg cagcgttttt aattgcgtcc tgcaaagtga
    31921 tttcataaac gtcgcgtgat tcgaaacgtt cgttatcttc cggggtaact gccacggaat
    31981 ggatgatgat ggtcgcttcg gcgatatcct gcgccgtcag gcgattctgt atgccgtccg
    32041 cgccctgggt ttcaattttc acttcataac cggcttctac cgccgcttct tccagcgcct
    32101 gagcggccat aaaagtgtga gccaggccca ttgggcaggc acataaggca atcagtttct
    32161 tactcatcgt taattcctcg ttcagtgata gttgacgagt gaaattatga acgtgcaaaa
    32221 caaatcgccg ttaccagaca aataatgcat ttactggaga attaatcccg ctgtacggaa
    32281 gtgtgacgca ggtcggcctt gtgaggggac acaaagaaaa attgaattaa ctgtatgatt
    32341 taaaagatta tcgggagagt tacctcccga tataacagga aggatttaca gaatgtgacc
    32401 taaggtctgg cgtaaatgtg caccggaacc gagaagaccc ggatagtcat ggacgatgag
    32461 atacaccgga atatcatgga catattcttt aaagcgccct ttatcttcaa atgcggcacg
    32521 gaaaccggag gctttgaaga actcaaggaa gcgcggcacg ataccgcccg caataaacac
    32581 gccgccaaat gtcccgagat tgagcgccag attgccgcca aaacggccca taatgacgca
    32641 aaacagcgac aatgcgcggc ggcaatcggt gcagctgtca gccagcgcgc gttcggtaat
    32701 atcttttggc ttgagatttt ctggcaggcg gttgtcagct ttcacaattg cgcgatacaa
    32761 attcaccagc ccagggccag aaagcacgcg ctccgccgaa acatgaccaa tttccgcacg
    32821 caatatttcg aggataatgg cctcttcttc actattcggc gcaaaatcaa cgtgaccgcc
    32881 ttcgcccggc aagcttaccc aacgcttatc gacatggacc agatgcgcaa ccccaagccc
    32941 cgttccggca ccgtaaaccg caataggctt accttcgacc ggttctgcgc caccaaactg
    33001 aatcagatgc tcttttttca gcatcgggat cgccatcgat acagcggtaa aatcgttaat
    33061 aatttccaga tggctaaaac cgagattctt tttcatttcg gcaattgaga acgcccaggt
    33121 atggttggtc attgccaccc agtcaccggt aattgggcaa gcgatggcaa tacagccgtc
    33181 tttcacctcg accttatgtt cttcaagata aacgcgaatg accgcttcga ggctggggta
    33241 atcaagccct gaataggtct tagcctgcga gatttcacca ctggcaatat cacacagagc
    33301 aagacgtgcg ttggtgccgc ccacatcacc gactaatgca tactttgtca ttcttcaact
    33361 gctccgctaa agtcaaaata attctttctc acactgtaaa tacctggggg cataacaaca
    33421 acgccggaaa ggcaggctcc ctgtaaatat cgatctgggt cacacaatta ctttatcgtt
    33481 tcagcaccaa ttgcagcgat gcctttttgc aagctgggca aactaagtat ctgaccccgc
    33541 ataaggaata gaacatgctc catccgcgag ccagaaccat gttgttatta tcgctccccg
    33601 ccgtggcaat tgggattgcg tccagtctta ttctgattgt ggtgatgaaa atcgcctcgg
    33661 tattacagaa tttgctctgg caacgactgc cgggaactct ggggatagcc caggattccc
    33721 ccctctggat catcggtgta ttaacgctaa cgggtattgc ggtggggttg gttatccgtt
    33781 tcagccaggg tcatgccgga ccagaccccg cctgtgaacc gctgatcggc gcaccggttc
    33841 cgccctctgc gctacctgga cttatcgtag cattaattct cggtcttgct ggcggcgtca
    33901 gcctggggcc ggaacatccg atcatgaccg tcaatatcgc ccttgccgtc gccattggcg
    33961 ctcgtctgtt accgcgcgtc aaccgaatgg agtggactat tttagcctct gccggaacca
    34021 tcggcgcgct gtttggtaca cctgttgcgg cggcgttgat attttcgcaa accttaaatg
    34081 gcagtagtga agttccgcta tgggatcgtc tttttgcgcc gttaatggcg gcagcagctg
    34141 gtgcacttac taccggatta tttttccatc cccatttttc actgcccatt gcgcattacg
    34201 gacagatgga aatgaccgat attctcagcg gtgcaattgt cgcggcgatt gccatcgcag
    34261 cagggatggt cgccgtatgg tgcttaccac ggttgcacgc gataatgaat caaatgaaaa
    34321 atccggtgct cgtgctgggt attggcggat ttattctcgg aattctgggg gttattggtg
    34381 gaccagtttc gctgtttaaa gggctggatg agatgcagca gatggtggca aatcaggctt
    34441 tcagcaccag cgattacttt ttgctggcgg taattaaact tgccgccctg gtcgttgctg
    34501 ccgccagtgg ctttcgcggt gggcgaatct tcccggcagt gtttgtcggc gtggcattag
    34561 ggttgatgct gcatgagcac gttcccgccg ttccagcggc aataaccgtt tcctgcgcta
    34621 ttctcggcat cgtgctggtg gtaacacgcg atggctggtt aagtcttttt atggcggcag
    34681 tcgttgtacc caataccaca ttgctaccgc tgctctgtat cgtcatgctt ccggcatggc
    34741 tgttattagc aggtaagccg atgatgatgg tcaatcgtcc gaagcaacag ccaccccacg
    34801 ataatgttta gcaaaaaatg ctctccttta tgatttaaga gttatggcga tgatacgggg
    34861 ctttacctcc ccgtaatatt gcgttaacag gccgctgacg gcttcgatcg tgaaggagaa
    34921 taacaatgtt caggtcactg tttctggcgg ccgccctgat ggcatttacc ccgcttgcag
    34981 caaatgcagg tgaaatcacc ctactgccat cgatcaaatt acaaattggc gatcgcgatc
    35041 attacggtaa ttactgggac ggtggtcact ggcgagatcg tgattactgg catcgcaatt
    35101 atgaatggcg caaaaaccgc tggtggcgtc atgataatgg ctatcatcgt ggctgggata
    35161 agcgtaaagc atatgagcgt ggctatcgtg aaggctggcg cgatcgtgac gatcatcgcg
    35221 gaaaaggccg cggacatggg caccgccatt aaacgcgtcc tccaatggag cacaatgcct
    35281 gatgcgacgc tcattcaact acaatcccag cgccgtcccc accaacaacc agatattcag
    35341 cgccacgacc agcactacaa tcacccagcc tgtctgtttt acgcgtttgc tgttcaccag
    35401 atcgcccatc aacttgctgt cactggtgaa aatcagcagt ggaaccagcg ccagggcgat
    35461 accaaaactt aacagcacct gactcataac cagaatccgt gtcggatcta atcccatcag
    35521 aatgacaata aatgacggca acatggtgac tgtacgacgc acccacagcg ggatatggaa
    35581 gcgaatgaat ccctgcatca ccacctgccc cgccagtgtc cccaccaccg ttgaggacag
    35641 tccggcagca accagactta acccaaagac cgttgccgca gcatggctta acagcggttg
    35701 cagcgtcaga taagcctcat caagatcggc aacaccagta tgaccagaaa agtggaacgc
    35761 cgccgcagct gtagccatca tcgccagatt gacaaaaccg gcaatcgtca tggcgatagc
    35821 cacatcccat ttggtggcgg aataacgttg ttgacgcgaa ccgccatgta aatgctgagt
    35881 gagcgaagag tgcaaataaa tcacatgcgg cataatcgtc gcccctaaca cgcctgctgc
    35941 caggaagacc gcctccgaag taggtaaact cgggatcacc attcctttac ccagctgcgc
    36001 caggttaggc tgggagaaaa tcaactcgac aatgtaagcc gcggcaacaa acaacagtaa
    36061 cccgccaatc actttctcca gcggtttttg cccgcgacgt tgcagcatta aaatcaggaa
    36121 agtcgcgatc cccgtcagca ccgcgccctg caacaacgaa acaccaagaa tgagtttaaa
    36181 accgatcgcc gcaccaataa attccgccag gtcggttgcc atcgcaataa tttctgcctg
    36241 aacccaatag aaccacacta cgggacgcgg atagtgatca cgaatctgct ccgccagatt
    36301 tttaccggtg gcaatcccta gtttggcaga gaggatctga attagcatcg ccatcaggtt
    36361 ggcccaaacg acaacccaca gtagctgata gccgaagcta gcacccgcct gaatattggt
    36421 cgcaaagtta ccgggatcga tataaccaat cgccgcaatg aacgcaggtc ccattaatgc
    36481 gagcctcatc ttgcgcgccg cccgtccgct gctactctca acgcgatagt tcgtcatctt
    36541 gtgcctctaa aacatagcct ttgctatgtt tcatgctatg ctaaacgaga atgattatca
    36601 aattcattta aatggattgt ggtgatttct ctgatagacc aagattatga ttacgaaaag
    36661 attgatgact tgaatgttat ggttatgttt aatgttagca catttacata actttcagct
    36721 tccatacaca acatagcaga aatgtatgac agatcactat ttttgaagcc tgtcacagga
    36781 cgtcattata gtgtgtgtca gatctcgttt tccttaacca tgttacatag aatgtgcacg
    36841 gaaatttaac ctgcctcata tttggagcaa atatggaccg cgtccttcat tttgtactgg
    36901 cacttgccgt tgttgcgatt ctcgcactgc tggtaagcag cgaccgcaaa aaaattcgta
    36961 tccgttatgt tattcaactg cttgttatcg aagtgttact ggcgtggttc ttcctgaact
    37021 ccgacgttgg tttaggcttc gtgaaaggct tctccgaaat gttcgaaaaa ctactcggat
    37081 ttgccaacga agggactaac ttcgtctttg gtagcatgaa tgatcaaggc ctggcattct
    37141 tcttcctgaa agtgctgtgc ccaatcgtct ttatctctgc actgatcggt attctccagc
    37201 acattcgcgt gttgccggtg attatccgcg caattggttt cctgctctcc aaagtcaacg
    37261 gtatgggcaa actggaatcc tttaacgccg tcagctccct gattctgggt cagtcagaaa
    37321 actttattgc ctataaagat atcctcggca aaatctcccg taatcgtatg tacaccatgg
    37381 ctgccacggc aatgtccacc gtgtcgatgt ccatcgttgg tgcatacatg accatgctgg
    37441 aaccgaaata cgtcgttgct gcactggtac tgaacatgtt cagcaccttt atcgtgctgt
    37501 cgctgatcaa tccttaccgt gttgatgcca gtgaagaaaa catccagatg tccaacctgc
    37561 acgaaggtca gagcttcttc gaaatgctgg gtgaatacat tctggcaggt ttcaaagttg
    37621 ccattatcgt tgccgcgatg ctgatcggct ttatcgccct gatcgccgcg ctgaacgcac
    37681 tgtttgccac cgttactggc tggtttggct acagcatctc cttccagggc atcctgggct
    37741 acatcttcta tccgattgca tgggtgatgg gtgttccttc cagtgaagca ctgcaagtgg
    37801 gcagtatcat ggcgaccaaa ctggtttcca acgagttcgt tgcgatgatg gatctgcaga
    37861 aaattgcttc cacgctctct ccgcgtgctg aaggcatcat ctctgtgttc ctggtttcct
    37921 tcgctaactt ctcttcaatc gggattatcg caggtgcagt taaaggcctg aatgaagagc
    37981 aaggtaacgt ggtttctcgc ttcggtctga aactggttta cggctctacc ctggtgagtg
    38041 tgctgtctgc gtcaatcgca gcactggtgc tgtaaaacca tacataaaaa agccggggat
    38101 cattccccgg cttttttatg cctgttaatc agctaatggc tgcgggcgac caatcaaata
    38161 cccttgcaga tattgcaccc cgagcttatg caatagcgcc tgctgctgtt gcgtctcgac
    38221 aaactccgcg accacactca atgacttcgc tttcgccaga tcggtaattg atctcacaat
    38281 catcgcatcc agcgtattcg tgacaatatc tttcacaaag acgccatcaa ttttgatgat
    38341 atcagcctgc aaacgcttta accgttcgta gttggcatat ccggcgccaa agtcatcaat
    38401 cgcaatccgg aaaccaaact tatgcagctg ctcgatgttg tacatgctgc tttctgcgtt
    38461 agaaaacgcc tgctcctcgg tgatctcaag aatgaccgcc tgcggggaga tgtgataacg
    38521 cttaaacaga cgaataatcc gcccggcaat attcttttgc agcagcgtga gcggcattaa
    38581 attgactgaa aagcgcggtc cttttttgtc gcaagggtgt gttgccagcc acttcaacaa
    38641 ggattccagc acttgcaaat caaaacgcgc gctaaggtta aactgagcaa taaggggcag
    38701 aaacttatcc ggggtcataa tgccgccgtc atatttcagt cgcgcgagga tctcatcata
    38761 accttcaccc tctttgttgc gaattggctg ggcgtagagc agcaaatcac cctgatctaa
    38821 cgccgtgcga atggtattca gcaatagcac ctgtttagtg gtctgcccgg aaaccatctc
    38881 ctctctgcta tccagcgcca gcacatgatg atgtgcgcag gattgctccg ccagccagct
    38941 taactgcccc aacaagggtt gcagggtttc ctgatttcca tcaaaacgcc cccaggcagc
    39001 accatagccc atatccagcc cggtattgtt ccagtgaatt tgccgactat tcaggatgtt
    39061 aaccatatgc tggagtcgcc cttccgtttc cggcccgctc agcactaaca gcagttcact
    39121 acccggcaat tgatacaact tttcgttttc ctgcatcaac ggcagcagcg tacggcaaat
    39181 tgagcggata cagtgaacgc gcatcattaa gccgtaatga cgactcataa actcaagatt
    39241 atcaatgcgc aggcagcaaa aactcttgcc cgcctcttgc tccggcgctt gttccaacgc
    39301 acgaaagttg ggtaggagcg ttaacggatc tgttagcgcc tgcaaatgcc agcggcgatt
    39361 aagccattca ctgcgatgat aaatgcgcac catatagagc aggcaaacgc taaaggaaat
    39421 cagcaccgcg agaataaatg ccagcgaata ttcggtttcc accccttgca aaaagttctg
    39481 gttgtaattc agaaggcaaa gcgttgaaac agcctaggtg agatttaaaa acggatagcg
    39541 aagattaccg accccgaggg taaagatgat gaagaaaacg ggtaccaggt aaccggcaat
    39601 aaagtcgttt tcataaggtg tgcacaacag aagtaggagc acgcttaacg ctgccagcca
    39661 gcttaaggta aatgcgcgtt tctctttgcc caacgacgga gcgatatccc tgcgccacaa
    39721 tatctgcgca aagtggggac ttacaatcat gcgagtgaga tagtagaaaa gcatgttgta
    39781 aatcagcact gcggtgaaaa ggcttagtaa atcaacgacc gtgaaaatgg catccgcatc
    39841 gccgaaaaag gtggatatct ttagcgggaa atcaaagaag ctccccacaa gatacatgct
    39901 gcatttgatg ccgatcggcg tcaccaaacc aagccagaac agacgttgcc agacatatcg
    39961 gctggtcaat ccataacgcc aacgcgtccc cagctgccag cgtaatattg cgcaggcaca
    40021 caacacgaca aacgtctggc tgccgagcat gaccgcagtc tgtaaaaaag agagatgaaa
    40081 gttccacaga ttggtgcaaa acatcccgca taaaacggga acgacaccgc gccagccaaa
    40141 gataaacaag attgacagca ttacgcacag tggcatccac gccagaaaga tataactgga
    40201 atggataatg gcaagtggcg aaataaaacg ggatagctga ataagtacca cggtgagcgt
    40261 aaacgctagt gtgaatatct tgatattttt tatcaggtta tgctccacaa acatgagtac
    40321 actactctca ctgatcgggg ttttcaggca cacaaataat aagtttattc gcatcgcgaa
    40381 gcaaacaggg gccagcctgt tgccgatttt tcatccaatg atgctttcac caggcggcgc
    40441 taacccggac gtaaactgta ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct
    40501 ttgcagcgag ggttggaaat ttggtggagc taagcgggat cgaaccgctg acctcttgca
    40561 tgccatgcaa gcgctctccc agctgagcta tagccccacg atgcgtttac g
//
LOCUS       DADMEQ010000021        38993 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.021, whole genome shotgun sequence.
ACCESSION   DADMEQ010000021 DADMEQ010000000
VERSION     DADMEQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.021"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            91..450
                     /locus_tag="JNZ05_001184"
     CDS             91..450
                     /locus_tag="JNZ05_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005072913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY8564488.1"
                     /translation="MYFLATAYKGNGIPANNEKYLAYLQQAATLGSSNAQAEIGYLYL
                     IGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDGVERNMEKARFHLSNAAEG
                     GIEPALKALNELEHQTK"
     gene            503..2233
                     /locus_tag="JNZ05_001185"
     CDS             503..2233
                     /locus_tag="JNZ05_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564489.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSDPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDEIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(2293..2784)
                     /gene="yajQ"
                     /locus_tag="JNZ05_001186"
     CDS             complement(2293..2784)
                     /gene="yajQ"
                     /locus_tag="JNZ05_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HAY8564490.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2952..3863
                     /gene="panE"
                     /locus_tag="JNZ05_001187"
     CDS             2952..3863
                     /gene="panE"
                     /locus_tag="JNZ05_001187"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAY8564491.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARTHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3826..4416
                     /gene="yajL"
                     /locus_tag="JNZ05_001188"
     CDS             3826..4416
                     /gene="yajL"
                     /locus_tag="JNZ05_001188"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HAY8564492.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(4470..5918)
                     /gene="thiI"
                     /locus_tag="JNZ05_001189"
     CDS             complement(4470..5918)
                     /gene="thiI"
                     /locus_tag="JNZ05_001189"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAY8564493.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            6124..6366
                     /gene="xseB"
                     /locus_tag="JNZ05_001190"
     CDS             6124..6366
                     /gene="xseB"
                     /locus_tag="JNZ05_001190"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAY8564494.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            6366..7265
                     /gene="ispA"
                     /locus_tag="JNZ05_001191"
     CDS             6366..7265
                     /gene="ispA"
                     /locus_tag="JNZ05_001191"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HAY8564495.1"
                     /translation="MDFPQQFEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            7290..9152
                     /gene="dxs"
                     /locus_tag="JNZ05_001192"
     CDS             7290..9152
                     /gene="dxs"
                     /locus_tag="JNZ05_001192"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAY8564496.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTICVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            9207..10181
                     /gene="yajO"
                     /locus_tag="JNZ05_001193"
     CDS             9207..10181
                     /gene="yajO"
                     /locus_tag="JNZ05_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HAY8564497.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRGDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPMARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(10235..10753)
                     /gene="pgpA"
                     /locus_tag="JNZ05_001194"
     CDS             complement(10235..10753)
                     /gene="pgpA"
                     /locus_tag="JNZ05_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAY8564498.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10731..11708)
                     /gene="thiL"
                     /locus_tag="JNZ05_001195"
     CDS             complement(10731..11708)
                     /gene="thiL"
                     /locus_tag="JNZ05_001195"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HAY8564499.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(11786..12205)
                     /gene="nusB"
                     /locus_tag="JNZ05_001196"
     CDS             complement(11786..12205)
                     /gene="nusB"
                     /locus_tag="JNZ05_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAY8564500.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(12225..12695)
                     /gene="ribE"
                     /locus_tag="JNZ05_001197"
     CDS             complement(12225..12695)
                     /gene="ribE"
                     /locus_tag="JNZ05_001197"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAY8564501.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12787..13887)
                     /gene="ribD"
                     /locus_tag="JNZ05_001198"
                     /pseudo
     CDS             complement(12787..13887)
                     /gene="ribD"
                     /locus_tag="JNZ05_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
     gene            complement(13891..14340)
                     /gene="nrdR"
                     /locus_tag="JNZ05_001199"
     CDS             complement(13891..14340)
                     /gene="nrdR"
                     /locus_tag="JNZ05_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HAY8564502.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            14491..15030
                     /locus_tag="JNZ05_001200"
     CDS             14491..15030
                     /locus_tag="JNZ05_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY8564503.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            15329..16213
                     /locus_tag="JNZ05_001201"
     CDS             15329..16213
                     /locus_tag="JNZ05_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HAY8564504.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKIRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(16390..16737)
                     /locus_tag="JNZ05_001202"
     CDS             complement(16390..16737)
                     /locus_tag="JNZ05_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HAY8564505.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16866..17837)
                     /gene="secF"
                     /locus_tag="JNZ05_001203"
     CDS             complement(16866..17837)
                     /gene="secF"
                     /locus_tag="JNZ05_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HAY8564506.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17848..19695)
                     /gene="secD"
                     /locus_tag="JNZ05_001204"
     CDS             complement(17848..19695)
                     /gene="secD"
                     /locus_tag="JNZ05_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HAY8564507.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19723..20055)
                     /gene="yajC"
                     /locus_tag="JNZ05_001205"
     CDS             complement(19723..20055)
                     /gene="yajC"
                     /locus_tag="JNZ05_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAY8564508.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(20078..21205)
                     /gene="tgt"
                     /locus_tag="JNZ05_001206"
     CDS             complement(20078..21205)
                     /gene="tgt"
                     /locus_tag="JNZ05_001206"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAY8564509.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(21260..22330)
                     /gene="queA"
                     /locus_tag="JNZ05_001207"
     CDS             complement(21260..22330)
                     /gene="queA"
                     /locus_tag="JNZ05_001207"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAY8564510.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            22423..22996
                     /gene="acpH"
                     /locus_tag="JNZ05_001208"
                     /pseudo
     CDS             22423..22996
                     /gene="acpH"
                     /locus_tag="JNZ05_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(23001..24818)
                     /gene="malZ"
                     /locus_tag="JNZ05_001209"
     CDS             complement(23001..24818)
                     /gene="malZ"
                     /locus_tag="JNZ05_001209"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HAY8564511.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYLFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQSEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPSCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(24974..26347)
                     /gene="proY"
                     /locus_tag="JNZ05_001210"
     CDS             complement(24974..26347)
                     /gene="proY"
                     /locus_tag="JNZ05_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HAY8564512.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(26423..27742)
                     /gene="brnQ"
                     /locus_tag="JNZ05_001211"
     CDS             complement(26423..27742)
                     /gene="brnQ"
                     /locus_tag="JNZ05_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HAY8564513.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(28149..29444)
                     /gene="phoR"
                     /locus_tag="JNZ05_001212"
     CDS             complement(28149..29444)
                     /gene="phoR"
                     /locus_tag="JNZ05_001212"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HAY8564514.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVFNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEASPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(29502..30191)
                     /gene="phoB"
                     /locus_tag="JNZ05_001213"
     CDS             complement(29502..30191)
                     /gene="phoB"
                     /locus_tag="JNZ05_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HAY8564515.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLLGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            30381..31583
                     /gene="sbcD"
                     /locus_tag="JNZ05_001214"
     CDS             30381..31583
                     /gene="sbcD"
                     /locus_tag="JNZ05_001214"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HAY8564516.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            31580..34723
                     /gene="sbcC"
                     /locus_tag="JNZ05_001215"
     CDS             31580..34723
                     /gene="sbcC"
                     /locus_tag="JNZ05_001215"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HAY8564517.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADMKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVELDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTTLQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34849..36030
                     /gene="araJ"
                     /locus_tag="JNZ05_001216"
     CDS             34849..36030
                     /gene="araJ"
                     /locus_tag="JNZ05_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HAY8564518.1"
                     /translation="MKKVILSLALGTFGLGIAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYLLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALFSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(36173..37081)
                     /gene="mak"
                     /locus_tag="JNZ05_001217"
     CDS             complement(36173..37081)
                     /gene="mak"
                     /locus_tag="JNZ05_001217"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HAY8564519.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            37206..38117
                     /gene="rdgC"
                     /locus_tag="JNZ05_001218"
     CDS             37206..38117
                     /gene="rdgC"
                     /locus_tag="JNZ05_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HAY8564520.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCSSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELHDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(38195..38650)
                     /locus_tag="JNZ05_001219"
     CDS             complement(38195..38650)
                     /locus_tag="JNZ05_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564521.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAAGRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(38647..>38993)
                     /locus_tag="JNZ05_001220"
     CDS             complement(38647..>38993)
                     /locus_tag="JNZ05_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HAY8564522.1"
                     /translation="QVFFAELATIKDKSDDEWWRALLQSRNDHLRQTALRNAHTPASL
                     LTTLTEPQDRSLAINNPQLAADVKTAWLKEDPSLLLFVEQPDLSQLRDLVKTGATRKI
                     RSEARHRLEEKQ"
BASE COUNT         9939 a        10314 c         9702 g         9038 t
ORIGIN      
        1 cattagggaa atacgaattc atctgtgcaa atatagatga agggattaaa tggttaacca
       61 aatctgcaga gcaaaaaaaa cactgatgcg atatactttt tagctacagc ctacaaaggc
      121 aatggtatac ctgcaaacaa tgaaaaatac ctagcatatc tgcaacaagc tgcaacactg
      181 ggcagtagta atgcccaggc ggaaattggc tatttatatc tcatcggaaa agaactacca
      241 cagaatcttc ctgatgctgg tgtctggttc aaaaaagcag ctgcacaagg taattttgcc
      301 gcacatttct atctgggaag gatgtatcag aatggtgacg gcgttgagag aaatatggaa
      361 aaagccaggt ttcatctatc taatgcggca gaaggtggaa tagaacctgc gcttaaagca
      421 ttaaatgaac ttgaacatca aacaaaataa agcgctcagg gaccacgtaa ggttcatcaa
      481 acagaaaagg atatagtttg cgatgaaagc gaaatctact ttgttattaa tgattttatc
      541 tgttttttat tctcatcagg ttttatccag cgaccccact cagccggatg tttattctgt
      601 cgtcgagaaa aaactaaact ctgctttacc tctggaagaa aataccacct tcagatcgca
      661 ggctgaatgg tttaatttgc aatatgaatt attagtatct ggctaccctg aacgtgctta
      721 caatttactg agcaaacttg agacagaatc gaaaaacgct aaatattatc tggatttaac
      781 tgcctccaga gtcgctgaag aagaagcacc agagacagcc ttaacttttt tatcaaaaat
      841 tgggttaaat aaaccggcta ctcttacatc ctttgagagc tatatcaata gctggataaa
      901 aagcaaacaa cccgaaaagg ctatgcagct tctcagcctc gatgagaatg ccctgaatta
      961 ttatttaccg actgtgttag tcgcatatca gatcgttcct gataaggcaa taagtattta
     1021 taattctgta tatggagata atgttgttat accctatcag caagtcacca tgttacttac
     1081 agttgcggag ggttatcaaa caaaaggcga tataaaaaac gccattcatt atgccgataa
     1141 ggcgctgaat atgtttgata aagctattgc tgtagatagc agtcgtgaat cccattatag
     1201 tgatgagtat ttgaaactca tgaatatcta cgccgccaat ggcaacaaag aaaaagcggt
     1261 ggttttatct caacgtctac acaaagccat taccgaatct caatcgaatt acttaagtac
     1321 tctggaagga attttattgt tttatagaaa taatgatatg cagcaagact atcaaaacag
     1381 cctttcaaat tacatagtat ttatcgatga aatttttgcc ttttccccat caacaagaag
     1441 tgaattaagc ctcattaatt tattaaattc attaaatgag gtggaattga tgagaaaacg
     1501 tctggcgttt ctgatgacag caccagaata cgcttgttat gatagtcagt attgttatga
     1561 agacaaagtc agcgcattaa aaatcctgta tcgtcatcat gacaatgagc taatggacaa
     1621 atatttcacg actcttctta aagagatcag taatcaacca ttagaagact gggatataat
     1681 aataagtaat actggtgaaa aatttagtga agtgggtcta accaaatatg ctcaacatct
     1741 cgcatctttc gctgaagaat tttatataaa agagcaaaaa tcacattctg aaagtgaaat
     1801 gagaggacta ttttcatcac ttgccgaatt atatagtttt ggtaatgata ctgccagcgc
     1861 aaaccgagtg ttccatcagc atgtccctgc cttcaataca gatcatatga ttcaatattt
     1921 tataaatgcc aaagaatgga acaatgccag ggaattattg ataaaagagg aaatgttggg
     1981 tatgcataat ctgatattgt tggagaatat ttgtatgcaa aaaaatgcag aatgcatggc
     2041 acacattacc tttacgctta ataaattaac aacacaacca gcaataacaa aaatagatag
     2101 cgtgggtaat gagcaacttt atcagattgg caaaatctac cataagttgg aaattaaacc
     2161 tgagccagag caacaggttt taattcagtc gctttatgac cgggcatcag gaagcgcctc
     2221 tgcaactcaa taaatgcatg gtcaaaaggg ccaccacggt ggccctgaca atcagtagct
     2281 atatccagaa agttaatcgc ggaagttttt gaactggaac ggctgaccga gatcgccacc
     2341 gcgcaccatc gccatgacag cctgcaaatc atcacgagat ttgccagtca cgcggatctc
     2401 atcgccctga atttgcgcct gcactttcag tttgctgtct ttgatcatct tgacgatttt
     2461 tttctgagtg gcgctctcaa tgccctgttt cagtttcgct tccacaaacc aggttttacc
     2521 gctatgaacg atattttccg gtacatccag tgaactgcct tcaatgccgc gcttcagcag
     2581 cttggcacgc agaatatcca gcaactgatt gacctggaag tcggactcgc tcaacacttt
     2641 gatggttttg ctggcgtcgt tcagctcaaa tgaggcttca acgttacgga agtcaaaacg
     2701 ggactccact tcgcgactcg cgttatcgac cgcgttacgt gcttcctgaa gatcaacttc
     2761 agagacaata tcgaaagatg gcatcttttc ctctcccttc atttttgatg cgaagcataa
     2821 tacctgcaaa gttaaataac caacagtcct gcaccaggac gtatccctta attgtgcata
     2881 gtcgcaacag catgcacaat taagggatag ccttattgaa cacccggagt ggttgtgggt
     2941 gaggaggaac aatgaaaatt accgtattgg gatgcggtgc cttagggcaa ttatggctta
     3001 cagcactttg taaacagggt catgaagttc agggctggct gcgcgtaccg caaccttatt
     3061 gtagtgtgaa tctggttgag acagatggtt cgatatttaa cgaatcgctg accgccaacg
     3121 atcccgattt tctcgccacc agcgatctgc tcctggtgac gctgaaagca tggcaggttt
     3181 ccgatgccgt caaaagcctc gcatccacac tgcctgtaac tacgccaata ctgttaattc
     3241 acaacggcat gggcaccatc gaagaattgc aaaacattca gcagccatta ctgatgggca
     3301 ccaccaccca tgccgcccgc cgcgacggca atgtcattat tcatgtggca aacggaatca
     3361 cgcatatcgg tccggcacgg caacaggatg gcgattacag ttatctggcg gatattttgc
     3421 aaaccgtgtt gcctgacgtt gcctggcata acaatattcg cgccgagctg tggcgcaagc
     3481 tggccgtcaa ctgcgtgatt aatccactga ctgccatctg gaattgcccg aacggtgaat
     3541 tacgtcatca tccgcaagaa attatgcaaa tatgcgaaga agtcgcggca gtgatcgaac
     3601 gcgaagggca tcatacttca gcggaagatt tgcgtgatta cgtgatgcag gtgattgatg
     3661 ccacagcgga aaatatctcg tcgatgttgc aggatatccg cgcgctgcgc cacaccgaaa
     3721 tcgactatat caatggtttt ctcttacgcc gcgcccgcac gcatgggatt gccgtaccgg
     3781 aaaacacccg cctgtttgaa atggtaaaaa gaaaggagag tgaatatgag cgcatcggca
     3841 ctggtttgcc tcgcccctgg tagtgaagag actgaagccg tcaccacgat cgacctcttg
     3901 gttcgcggcg gtatcaaagt caccactgcc agcgtcgaca gcgatggcaa cctggcaatt
     3961 acctgctcgc gcggcgtgaa gctgctggcg gatgcgccgc tggtcgaagt ggctgatggc
     4021 gaatatgacg tgatcgtgct gcctggtggc attaaaggca cggagtgttt tcgcgatagc
     4081 actctgctgg ttgaaaccgt taaacagttc caccgttccg ggcgtatcgt cgcggctatt
     4141 tgcgccgcgc cagccaccgt gctggtgccg cacgatatct ttccgattgg caatatgacc
     4201 ggcttcccga cgctgaaaga caaaattccc gctgaacaat ggcaggacaa gcgcgtcgtc
     4261 tgggatgcac gggtaaaatt gctgaccagc caggggccgg gtacagctat cgactttggt
     4321 ctgaaaatta tcgacctgct ggttgggcgc gagaaagccc atgaagtggc atcacaactg
     4381 gtgatggcgg cagggattta taattattac gagtagtgtc ggatgcggca aacgtcgcat
     4441 ctgaccagat gcgacgtaaa aactacaaat tacgggcgat ataccttcac attgttaaag
     4501 ccttgctcgc gcagatagag cgcctgcaga cggctcatca ccccgcgctc acaccacagc
     4561 agccaggttt tgttctggtc gagatcgcca aatttggtgc tcagtttata gaacggcaga
     4621 gaaaccacat caatcccttc gactttcagt ggcttatctt cctgttcatc gacagaacgg
     4681 atatcgagaa tcacgtcgtt cgggccgaag ccgttgacgg tttccacttc caccacttcc
     4741 tgctcggtct gctgggcgat ttcgcggata tcaacgttat tcgcttcctc aaccacttta
     4801 tcgagaatac tgaagtcgaa cttctcttct tccgcttcaa tcttcgattt aaccgctttc
     4861 accgtcgggc ttttggagat cacgccacaa tattccggca tcgtgcgagc aaagtcttcg
     4921 gtgccaatct ggcgagccag gttgatgatg tgctctttgt cgtaagaaat cagcggacgc
     4981 aggatcagcg tgtcagagac gttatcaatc aggcgcaggt tggtcagcgt ctgactggac
     5041 acctggccga gcgcttcgcc ggtgaccagc gcctgtacgc cgtaacgttc agccacttta
     5101 gatgcggcac gcaccatcat acgtttgagg ataacgccca tctgaccgtc gtcgattttc
     5161 tcgagaattt ccccgacaac cggttcgaaa ttaatggcga caaaacgcac gcggtgggag
     5221 ctgccaaagc ggttccacag ataatgcgcc acctgacgca cgccaatttc atgcgctgcg
     5281 ccgccgaggt taaagaagca gtaatgcacg cggcagccgc gacgcatcaa catataactg
     5341 gaaacaccgg agtcgaaacc accggaaatg agcgacagca catcttcctg ggtgccaatc
     5401 gggaaaccac caataccttc gtagcgacct ttaatcagca ggaggcgatc gtcttccact
     5461 tccagatgaa cagttacatc cgggttagtc agcttcacgc gcgcggattc aatatgctga
     5521 tttaaaccgc cgccgacgta acgttccaca tcaatcgagc taaaatcatg tttgccacgg
     5581 cgcttcacgc gtacgcagaa ggttttgcct tccagctgat cgcgatactg aaccaacgct
     5641 ttctcaaaaa tatcgtgcat gtcggtaaac ggcacgtctt cgacttcgag aatatggtgg
     5701 atacccggaa tacgggtcag ggcgtcgcga atagtcagac gctggttttc atctttcgcg
     5761 cgaacttcga tgttatccca gtggcggacg acagcgagcg tctcatcata gtgctttaaa
     5821 acgttacgaa tgttcccggt aaggattttt ataaagcgca agcgcacaga ttggcttttg
     5881 atggtgattt ccgggaacaa tttaatgata aacttcatgg cggcaatggt tcgttggcaa
     5941 gccttaagcg acttgtatag ggaaaaatac agcagcccac acctgcggct gcatccaggc
     6001 gcggaagtat accactaaca tcgctttgct gcgcacatca ccttaccatt gcgcgttatt
     6061 tgctatttgc cctgagtccg ttaccatgac ggggcgaaaa atattgagag tcagacattc
     6121 attatgccga agaaaaatga ggcgcccgcc agctttgaaa aggcgctgag cgagctggaa
     6181 cagattgtaa cccgtctgga aagtggcgac ctgccgctgg aagaggcgct gaacgagttc
     6241 gaacgcggcg tgcagctggc acgtcagggg caggccaaat tacaacaagc cgaacagcgc
     6301 gtacaaattc tgctgtctga caatgaagac gcctctctaa ccccttttac accggacaat
     6361 gagtaatgga ctttccgcag caattcgaag cctgcgttaa gcaggccaac caggcgctga
     6421 gccgttttat cgccccactg ccctttcaga acactcccgt ggtcgaaacc atgcagtatg
     6481 gcgcattatt aggtggtaag cgcctgcgac ctttcctggt ttatgccacc ggtcatatgt
     6541 ttggcgttag cacaaacacg ctggacgcac ccgctgctgc cgtagagtgt atccacgctt
     6601 actcattaat tcatgatgat ttaccggcga tggatgatga cgatctgcgt cgcggtttgc
     6661 cgacctgcca tgtgaagttt ggcgaagcaa acgcgattct cgctggcgac gctttacaaa
     6721 cgctggcgtt ctcgattcta agcgatgcca atatgccgga agtgtcggat cgcgacagaa
     6781 tttcgatgat ttctgaactg gcgagcgcca gcggtattgc cggaatgtgc ggtggtcagg
     6841 cattagattt agacgcggaa ggcaaacacg tacctctgga cgcgcttgag cgtattcatc
     6901 gtcataaaac cggcgcattg attcgcgccg ccgttcgcct tggtgcatta agcgccggag
     6961 ataaagggcg tcgtgctctg ccagtactcg acaagtacgc agaaagcatc ggccttgcct
     7021 tccaggttca ggatgacatc ctggatgtgg taggagatac tgcaacgttg ggaaaacgcc
     7081 agggtgccga ccagcaactt ggtaaaagta cctaccctgc acttctgggt cttgagcaag
     7141 cccggaagaa agcccgggat ctgatcgacg atgcccgtca gtcgctgaaa caactggctg
     7201 aacagtcact cgatacctcg gcactggaag cgctagcgga ctacatcatc cagcgtaata
     7261 aataaacaat aagtattaat aggcccctga tgagttttga tattgccaaa tacccgaccc
     7321 tggcactggt cgactccacc caggagttac gactgttgcc gaaagagagt ttaccgaaac
     7381 tctgcgacga actgcgccgc tatttactcg acagcgtgag ccgttccagc gggcacttcg
     7441 cctccgggct gggcacggtc gaactgaccg tggcgctgca ctatgtctac aacaccccat
     7501 ttgaccaatt gatttgggat gtgggacatc aggcttatcc gcataaaatt ttgaccggac
     7561 gccgcgacaa aatcggcacc atccgtcaga aaggcggtct gcacccgttc ccgtggcgcg
     7621 gcgaaagcga atatgacgta ttaagcgtcg ggcattcatc aacctccatc agtgccggaa
     7681 ttggtattgc ggttgctgcc gagaaagaag gcaaaaatcg ccgcaccatc tgtgtgattg
     7741 gcgacggcgc gattaccgct ggcatggcgt ttgaagcgat gaatcacgcg ggcgatattc
     7801 gtcctgatat gctggtggtc ctcaacgaca atgaaatgtc gatttccgaa aatgttggcg
     7861 cgcttaacaa ccatctggct cagctgcttt ccggtaagct ttactcttca ctgcgcgaag
     7921 gcgggaaaaa agttttctct ggcgtgccgc ccattaaaga gctgctcaaa cgtaccgaag
     7981 aacatattaa aggcatggtg gtgcctggca cgttgtttga agagctggga tttaactaca
     8041 tcggcccggt ggacggtcac gatgtgctgg ggcttatcac cacgctaaag aacatgcgcg
     8101 acctgaaagg cccgcagttc ctgcatatca tgaccaaaaa aggtcgtggt tatgaaccgg
     8161 cagaaaaaga cccgatcact ttccacgccg tgcctaaatt tgatccctcc agcggttgtt
     8221 tgccgaaaag tagcggcggt ttgccgagct attcaaaaat ctttggcgac tggttgtgcg
     8281 aaacggcagc gaaagacaac aagctgatgg cgattactcc ggcgatgcgt gaaggttccg
     8341 gcatggtcga gttttcacgt aaattcccgg atcgttactt cgacgtggct attgccgagc
     8401 aacacgcagt gacctttgcc gccggtctgg cgattggtgg atacaaaccc attgtcgcga
     8461 tctactccac cttcctgcaa cgcgcctatg atcaggtgct gcatgacgtg gcaattcaaa
     8521 agctaccggt cctgttcgcc attgaccgcg cgggcattgt tggtgctgac ggtcaaactc
     8581 accagggcgc ttttgatctc tcttacctgc gctgcatacc agaaatggtc attatgaccc
     8641 cgagcgatga aaacgaatgt cgccagatgc tctataccgg ctatcactat aacgatggcc
     8701 cgtccgcggt gcgctacccg cgcggcaacg cggttggcgt ggaactgacg ccgctggaaa
     8761 aactaccaat tggcaaaggc attgtgaagc gtcgtggcga gaaactggtg atccttaact
     8821 ttggtacgct gatgccagaa gcggccaaag tcgccgaatc gctgaacgct acgctggtcg
     8881 atatgcgttt tgtgaaacca cttgatgaag cgttaattct ggaaatggcc gccagccatg
     8941 aagcgctggt caccgtagaa gaaaacgcca ttatgggcgg cgcaggtagc ggagtgaacg
     9001 aagtgctgat ggcccatcgt aaaccagtac ccgtgctgaa cattggcctg ccggacttct
     9061 ttattccgca aggaactcag gaagaaatgc gcgccgaact cggcctcgat gccgccggta
     9121 tggaagccaa aatcaaggcc tggctggcat aatccctact ccactcctgc tatgcttaag
     9181 aaattattca tggcaggagt ggcagcatgc aatacaaccc cttaggaaaa accgaccttc
     9241 gcgtttcccg actttgcctc ggctgtatga cctttggcga gccagatcgc ggtaatcacg
     9301 catggacact gccggaagag agcagccgtc ccataattaa acgcgcgctg gaaggtggca
     9361 taaatttctt tgataccgcc aatagctatt ccgatggtag cagcgaagag atcgttggtc
     9421 gcgcactgcg ggatttcgcc cgccgtgaag acgtagtcgt tgccaccaaa gtgttccatc
     9481 gcgttggtga tttaccggaa ggattatccc gtgcacaaat tttgcgctct atcaacgaca
     9541 gcctgcgacg tctcggcatg gattatgtcg atatcctgca aattcatcgc ggggattaca
     9601 acacgccgat cgaagagacg ctggaagccc tgaacgacgt ggtaaaagcc gggaaagcgc
     9661 gttatatcgg cgcgtcatcc atgcacgctt cgcagtttgc tcaggcgctg gaactacaaa
     9721 aacagcactg ctgggcgcag tttgtcagta tgcaggatca ctataatctg atttatcgcg
     9781 aagaagagcg cgagatgctg cctctgtgtt atcaggaggg cgtggcggtg attccgtgga
     9841 gcccgatggc gcgggggcga ctgacgcgtc catggggaga aactaccgca cgactggtgt
     9901 ctgatgaggt ggggaaaaat ctctataaag aaagcgatga aaatgacgcg cagatcgcag
     9961 agcggttaac gggcgtcagt gaagaactcg gtgcaacacg agcacaagtt gcgctggcct
    10021 ggttgttaag taaaccgggc attgccgcac cgattatcgg aacttcgcgg gaagaacagc
    10081 ttgatgagct gctgaacgcg gtggatatca ctttgaagcc agaacagatt gccgagctgg
    10141 aaacgccgta taaaccgcat gcggtcgtag gatttaaata agagtccagg cctgatgaga
    10201 cgtgacaagc gtcacatcag gcatcggtgc acaactacga cagaataccc agcggccagt
    10261 gatgaccgat aaagtacagg atgcctgcgg aaatcacccc ggcgacaata tcgtcgatca
    10321 tgatccccat gccgccatgt acattgcgat caaaccagcg gatcggccac ggcttccaca
    10381 tatccagaat acggaaaatc acaaacccgg cggcaaccca ctgccagtca ttggtcggca
    10441 gcgccatgag cgtgatccac ataccaataa attcgtccca gacaatgctg ccgtgatcgt
    10501 gcacgcccat atctttcgcc gtttgatgac aaagatagac gccgatacag atccccaaca
    10561 tcaccaccag cgagtagagc tgccagggta aaaaggtcat cagataccag aaagggatcg
    10621 ccgccagcga tcccatcgtg ccaggaacga ttgggcttaa tccacttccg aatccgacag
    10681 caagcagatg ccacggatta ctcatcttca ggcgactttt cgcgacatct ttatggcgtg
    10741 acaaaatggt catatccttt ccagtcaaac gtgacaggtt cgccgtcacg aataaaacaa
    10801 agcccttcga tatcggcggt catttgcccg atacaggtaa acggtacgcc aaggtgtccg
    10861 agagcaacat ccagcgcgcc acggttcagt tccggcaccg tgaaacacaa ctcgtaatct
    10921 tcaccgccag agagcgccca gcgcagcgcc tgttccggtt caacatggcg agaaagcgca
    10981 tcagaaaacg gcagcaatgc caggtcaata cgtgcgccgc agtcgctggc tttcacgata
    11041 tgcccgagat cggaaatcag accgtcagag agatcgatag ctgaatttgc cagatcgcgc
    11101 agtgcctgcc cctgtaaaat acgcggcgat ggacggagat gacgtttgat caagtagtca
    11161 gcatctttag catcggcaac ctgcaaacgg ttttgcaaaa tcgccagccc ggcggcgcta
    11221 tcgcccggtg taccggtcac atagatccag tcacccggtt tcgccccaga gcgcgttaag
    11281 gctcgtccca tcggaacaaa gccgtggata cccaacgtca ttgataatgg cccacgcgtg
    11341 gtatcgccgc caatgagttg catatcgtaa taattgagaa gatcaaacaa actgtcgctg
    11401 aaggactcaa gccacgcttc gtctacgtcc ggtaaggtta atgccagcgt cagccaggcc
    11461 ggatcggccc ccatcgctgc cagatcgctt aggttcaccg ccagtgcttt ataagccaga
    11521 tcagcaggat cgatatcagg gaggaaatgg ttgcccgcca ccagcgtatc agtgctgatc
    11581 gccagggtct gtttctcggg gatattgaga agagcgcagt cgtcgccgat gcccagttcg
    11641 acatcaagac gagaacttct tacacggtca aaataacggg caatcaggga gaactcgcca
    11701 catgccatac gttatgcctc agcaggtaaa aaagaaaagg ccggcgatca tggaacagtc
    11761 ttccgtgaat ctaccggcct ggatattact ttttgttagg gcgaatcaca ggtgctgctt
    11821 tatcgagtac gccgttgacg aacttatggc tgtcttctgc gccgaacgat ttcgccagtt
    11881 cgatcgcttc gttaatggcc actttgtatg gcacatcact acgtttagac agttcgtaca
    11941 gcgcaatgcg cagtactgct ttttctacct ggcccagttc ttccagcagg cgggacaggt
    12001 agggcttcat cagtccgtcg aggtatgcgg tgttagtcgc caccccggcc agcagctcac
    12061 ggaagtacag gacgtcaacg tcttttacat cctgttcagc caggaactgg tattcaacat
    12121 cagcgatgtc gttctgggac aactgccagg agtagagcgc ctggacggca cactcacgag
    12181 cgcggcgacg agcagcaggt ttcacggatt tccccttact aatttcaggc cttgatggct
    12241 ttcaatacat taatcatttc aagcgcggtc agtgcagctt ctgcaccttt gttgccagct
    12301 ttggtgccag cacgttcgat cgcttgttca atgctttcag tggtcagaac cccaaaagca
    12361 accggaattt cgctgtcctg ggcaacatgc gccaggccgt tgcttgcacc accagcgaca
    12421 tattcaaagt gggcagtgcc accacgaata accgtaccca gcgcaatcac cgcgtcgtat
    12481 ttaccggttt tagccagtgc acccgccgcc agcggcagct cataggcacc aggcacccaa
    12541 acaacggtaa tgttttcatc ttttacctga ccgatacgtt tcagtgcgtc aattgcacct
    12601 tccagcaggc tgtcattgat aaagttgttg aaacgcgcga tggtgatggc gacgcgagcg
    12661 tccggggtag caacgttagc ttcaataatg ttcattttct tccttcgggt tcgagtatgg
    12721 ccccgcaggg gggcggattt tagcataata tttcgtgcgc tgcttccctt tcgagccggg
    12781 agagcatgcc cccactaaat gcaggcaaac atccgggcct acatgacgta tctctttgaa
    12841 tttaaattgg ggggcgtcgg ctaatttctc aagccctggc agcgagcata atccacgggc
    12901 gtcgctgcct aatagtttag gtgcgatata gacaatcagt tcatcgacta aacccgcctg
    12961 tagcaatacg ccagcgagcg ttggccccgc ttccacccag atgctgttaa tctgctgttt
    13021 acccagttgc atcatcagta caaccagatc cagatgacct ttatgctctg gaatcagcaa
    13081 ggtacgcacc gtttccggcc actcacgaga atcttcctgc gtacgcgcga accaggtttc
    13141 gccgggctgc tgcacaatgc gatgttccgg cgtcacgcga ttttggctat caatcacaat
    13201 acgtactggc tggcggagat tttgttgcgg gtagagcgcc tgagtttgtt catccagttc
    13261 agaccaacgc actgttaagg ctggatcatc cgccagcacc gtggcgctgc tggttaaaat
    13321 ggcatgactt tgtgcgcgct gtcgttgtac gtcgcgccgc gcctgaggcg aagtgatcca
    13381 ctggctttcg ccgctcgcca tcgccgtgca gccatcaagc gatgcgccaa gttttaactg
    13441 aatataagga aagccggtgc gcatccgctt gagaaagcct ttattcaatt gctcggcttc
    13501 actcatcatc agcccgtggc tgacgtcaat gccagcctgt tgcagacggt aaagtccacg
    13561 cccagcgacc tgcgggttag gatcttgcat cgctgcgacc acgcgcgcta cgccagcggc
    13621 gatgagtgcg tcacagcacg gtggcgtacg gccatgatgg ctacagggtt cgagtgtcac
    13681 atacgccgtt gcgcctttgg ctttttcacc cgccatacgc aacgcgtgta cttcggcatg
    13741 tggttcaccc gctcgttggt ggtaaccttc accgacaatt tcgccatctt tgacaatgac
    13801 gcacccgaca ttcgggttgg ggtgggtggt aaaacgtcct cgttgcgcca gctttagcgc
    13861 ccgcgccatg taatactcgt cctgcacggc ttagtcctcc aggcgcgcga tctcttcgcc
    13921 aaattctttg atatcttcga aactgcggta gacagaggca aaacggatat aggcgacttt
    13981 atcgagcttt ttcaattgct ccatcaccag attgccaatc atcttgctcg gtacttcgcg
    14041 ctcaccggtg gcgcgcagct gcgatttaat atgattgatt gccatttcaa cgtaatcgga
    14101 actcaccgga cgtttttcca gcgcccgcag cattccgcta cgcaatttct cttcattaaa
    14161 cggttcacgc acgtcgttgc tttttacaac acgcggcata accagctccg ccacttcaaa
    14221 ggtggtgaaa cgttcattac acaccagaca ctgccgacgg cggcgtacgg atgaaccctc
    14281 gcccacgaga cgagagtcaa ttaccttagt gtccacggcg aaacagaatg ggcaatgcat
    14341 acggcgtcct gaccaggtgg ttaacggaaa tctattttac cctgaactga cgatacaaca
    14401 aaggcgcaac gcttttgaga taaaggatcg atgctttcgc tgcttttacc ctctaccatt
    14461 aggctaattg cgacgagaaa gggacgacct atgaatacta acgtttttcg actgctcctg
    14521 ctgggaagcc tgttcagcct tagcgcctgt gtgcagcaaa gtgaagtgcg acagatgaaa
    14581 cacagcgtca gcacgctgaa ccaggagatg acgcaactca atcaagaaac agtcaaaatc
    14641 actcagcaaa acaggctgaa tgcaaaatcc agcagtgggg tttaccttct gcctggagcg
    14701 aaaacaccgg caagactgga aagccagatc ggtactttac gtatgtcgct ggtgaatatt
    14761 acgcctgata cagatggcac cacccttaca ctacgtattc agggtgagtc gaatgatccc
    14821 ttgcccgcat tcagcggaac tgttgaatat gggcaaattc agggaacaat agacaacttt
    14881 caggaaatca atgtgcagaa tcaattgatt aatgcccctg ccagcgtcct cgcccccagc
    14941 gatgttgata ttccgttaca attaaagggg atttctgtgg atcagttagg ctttgtacgt
    15001 atccacgaca ttcaacctgt tatgcagtaa acgtatttcg ggacgatttt gtgcctcccg
    15061 caacatcttt ccccgtcatt ttgttactct gcttacatca cttggattga tagtaaaagt
    15121 ttgcaacaag ggcgaaagtc agtacaatcc ccgcccgaat gtgtgtaaac gtgaacgcaa
    15181 tcgattacgt aaatgataga actgtgaaac gaaacatatt tttgtgagca atgattttta
    15241 taataggctc ctctgtatac gaaatattta gaaacgcaat ttgcgccttt ttcactcccg
    15301 caagggattt tcaaacagtg gcatacatat gaaaaaaaca ttactggcag ccggtgcggt
    15361 actggcgctc tcttcgtctt ttactgtcaa cgcagctgaa aacgacaaac cgcagtatct
    15421 ttccgactgg tggcaccaga gcgttaacgt tgtcggaagc tatcacaccc gtttcggacc
    15481 gcagatccgc aacgatacct accttgagta cgaagcattc gctaaaaaag actggttcga
    15541 cttctatggt tatgcggatg cgccggtatt cttcggcggt aactccgatg ctaaaggtat
    15601 ctggaaccac ggttctccgc tgtttatgga aatcgaacca cgtttctcca tcgacaagct
    15661 gaccaatact gaccttagct tcggtccgtt caaagagtgg tacttcgcga acaactacat
    15721 ttacgacatg ggtcgtaata aagatggtcg ccagagcacc tggtacatgg gtctgggtac
    15781 cgatatcgac actggcctgc cgatgagcct gtccatgaac gtctatgcga aataccagtg
    15841 gcagaactat ggcgcagcga acgaaaacga gtgggacggt taccgtttca aaattaaata
    15901 ctttgtgccg attaccgatc tgtggggcgg tcagctgagc tacatcggct tcaccaactt
    15961 cgactggggt tccgatttag gggatgacag cggtaacgca atcaacggta ttaagatccg
    16021 tactaataac tctatcgctt ccagccatat tctggctctg aactacgatc actggcacta
    16081 ctctgtcgta gctcgttact ggcacgacgg tggtcagtgg aacgacgatg cagaactgaa
    16141 cttcggcaac ggcaacttca acgttcgctc taccggctgg ggtggttacc tggtagtagg
    16201 ttacaacttc tgattatgaa aatgccggga tttattcccg gcatttctga ttgttaacaa
    16261 cgtgtgcttt gttcatgccg gatgcggcgt aaacgctttg tccggcccac aaaagcacgt
    16321 aaattcaata tattgctgaa ataacgtagg cctgataagc gtagcgcatc aggcaatttt
    16381 acgttttaat cacttcttct tgttcatcat cgctttcagg tcagcgaatg ggttgtactt
    16441 cgcttcaccg acatctttct gcgcatcttc ccctgcgata acggtcgtac catactgatc
    16501 cgcttcggta tatttcgaat gctcatgatc gtggcaatag agacacaaca attcccagtt
    16561 actgccatct tccgggttat tggtatggtc gtgatcaatg tggtgaacgg taagttcacg
    16621 caggttagaa taaacaaact cgcgggaaca gcgaccgcag acccacggat agatttttaa
    16681 tgctttttcg cgatagccgc tttctaaccg tgcatagttt tttgggatga tagccatgaa
    16741 aagttacctg gatgaaatgt agggttacgc gcagtttccc aagatgcggg gattttcgca
    16801 atctccatac atcaaaaaaa atcccgatct tctgaccggg attttcaaca tcaacgggaa
    16861 cttgattaag gcagaattga cggctgatcc gccccttctt tttccacttt ctgctgcaac
    16921 atgtgttcgc gcttcatacc cagtttcaga gccaacgcag atgctacgta gatggaagat
    16981 gcagtgccga tggaaacacc gataagcatg gtcagcgaga agccttccag taccggacca
    17041 ccgaagagat acagcatcag gataaccatc aaggtagtac cggatgtgat caaggtacgg
    17101 tgcagcgtct gggtcaagga cacgttaaag atttcgtaag gcgtaccgcg acggatcttg
    17161 cggaagtttt cacgaattcg gtccgatacc acgatactgt cgttaagcga gtaaccgata
    17221 accgacatca acgatgccac aatggtcagg tcaatctcga tatggaataa cgacaaaata
    17281 cccagcgtaa tgataacgtc gtgcgccagc gcaataacca cccctgccgc cagtcgccac
    17341 tcaaagcgga aacctacgta cacgaggata gacagcagcg ctgccatcaa cgccatcgca
    17401 ccggtttgcg caaggtctgc ccccacgctc ggaccgacga actcaatacg cttcactgct
    17461 gcattctgat tggtggattc gttaatcacc ttcagaactt ggctgcccag cacctgaccg
    17521 ccggtttcgc cttcagcagg cggcatacgg accatgatgt cgtggctgct accaaagttt
    17581 tgcagcattg gctcttcaaa accggctttt tgcaatgcat cacgcattac gtcaatttca
    17641 gccggttttt cgagcgtaat ttcgataacc gtaccaccgg tgaaatccag cccccagtta
    17701 aagccgcgca cgcccataat aacgatagca gcgattaaca gcagaccaga gatgccgaaa
    17761 gcccagtagt cccagcgcat aaagtcatag actttacggc cgtggtttag ttgttcaaca
    17821 gtatattcct gtgccacatc gcactcctca gattgacagc ttcttgacgc gcttgccgcc
    17881 atatagcagg tttacgatgg cacgcgtacc gacaatcgcg gtaaacatcg acgtcgccac
    17941 accgataccg gtagtaatcg cgaacccttt aattgccccg gtacccactg cgtacaggat
    18001 gatgacttta atcagcgtgg tgatgttcgc atcgaagata gaactgaatg cgccacgata
    18061 accttcatca attgcctgtt gaacagtacg tccgttgctc aactcttctt taatacgttc
    18121 gttgatcagt acgttcgcat cgaccgctac cgcaagggtt aagacgatac ccgcgatacc
    18181 tggcatactc agtgttgcgc ctggcaacag cgacataata ccgacgatca agatcaagtt
    18241 ggcaatcaga gcactggtcg caatcagacc aaacttctta tagaagatga tcatgaacag
    18301 aatagaaacc agcagaccgg caaggcaagc ctccagcccc tgctcaatgt tctgcatacc
    18361 cagggtcggg ccaattgtac gttcttcaac aatctgaatc ggcgcgatca acgcaccggc
    18421 acgcagcagc agtgacagct gacgggcttc gttcgggttg ttgatgccgg tgatacggaa
    18481 gctgttaccc agacgagact ggatgttggc gatgttaatc acctcttcct gtttcaccag
    18541 aaccgcacga ccatttgcat ctttcttacc gctgtctttg tactccacaa acagggttgc
    18601 catcggtttg ccgatgttgt ccttggtgaa gttagacata atgttgccac cagcgctatc
    18661 gagcgagatg ttaacctgcg gctggttgta ttcgtcctgg ctggaagtgg agtcggtgat
    18721 atggtcacca gtcagaatta cgcgtttgta cagcacaacc ggctgacctt cgcgggtctg
    18781 tttcacttca gagtcgcccg gtacgcgacc ggatgccgca gcggcctgat caacattggt
    18841 gtttaccaga cggaattcca gcgttgccgt cgcgcccaga atctctttcg cacgcgcagt
    18901 gtcctgaata ccaggcagtt caacaacgat acggtcagca ccctgacgct gaaccaccgg
    18961 ctccgccacg ccaagttggt ttacacggtt acgcaggata ttaatgttct gctgcaccgc
    19021 atattcacgc gcttcactca gacgagcatc gctcattacc gcacgcagct ggttgctgcc
    19081 ctggctgcta atcaccaggt ccggatggcg cttgctcaga tacgcaatgg cttcatcacg
    19141 agctttagca tcgcggaaag tgatgctcag gccgtagttg ttttctttac gaacagtggt
    19201 atacgggatg cccttttcgc gcaggtcact gcgtaggcta tcgatatttt gttcctggag
    19261 tttgccaagc gcggtgtcca tatccacttc catcaggaag tgaacgccgc cacgcaggtc
    19321 aaggccgagc ttcatcggct cagcatgaat agctgccagc cagcgcggcg ttgccggggc
    19381 aaggttaagc gccacgacgt atttgtcacc cataacgccc attaatgctt cacgagcgcg
    19441 caactgggtg tcagtggagt cgaagcgcgc aagaatagcg ccctcttcca gtgccacaga
    19501 cttagcagtt attttttctt cttgtaacgt tttctggacc tggatcagcg tttgctcact
    19561 ggcggcgact ccgcgcgcac cagtgatctg aacagccgga tcctcaccaa acaggttggg
    19621 aagcgcatac agcagaccga tgacaatcac cacgatcagc atgacgtact tccacaaagg
    19681 ataacggttt aacacggcaa ttcccttagg gaaaaatttt aattacagcg ccttcatggt
    19741 gcctttcggc aggacggcag ctacgaagtc acgtttaata actacttcag tggtgtcatt
    19801 cagcgcgata gcaatgtagc cgttttccgc tactttggtt acgcgaccaa ccaggccacc
    19861 gttcgtcaga acttcatcac ctttggcaat ggagtccatc agctttttgt gttctttggt
    19921 gcgcttctgc tgtggacgca ggatcatgaa atagaaaatc agaccgaata ccaccagcat
    19981 caaaatcaaa gacatcgggc taccttgcgc cggtgcaccc gttgccgcta ccgcatcaga
    20041 aataaaaaag ctcattaaat ttccctcatt attaatatta atcaacgttc aaaggtggta
    20101 cttctcgccc ctgacgctgg taaaaatcag ttacgaagct ctctaattta ccctcttcaa
    20161 tagccttgcg taaacccgcc atcaaacgct ggtagtaacg aaggttatga atggtgttga
    20221 gtcgcgcgcc taatatttcg ttgcaacggt cgagatgatg caagtaagcg cgtgaataat
    20281 tgcgacaggt gtagcaatca cactcaggat cgagtgggcc agtatcgctc ttatatttcg
    20341 cattgcggat tttcaccacg ccatcggtca cgaacaaatg accatttcgg gcgttgcggg
    20401 ttggcattac gcagtcaaac atatcgatac cgcgacgtac gccttcaacc aggtcttccg
    20461 gtttaccaac gcccatcagg taacgcggtt tgtctgccgg aatttgcgga cacacatgct
    20521 ccagaatacg gtgcatatct gctttcggct cacccacagc cagaccgccg acagcgtagc
    20581 catcaaagcc gatatctacc agacctttaa cagatatatc acgtaaatct tcgtaaatac
    20641 tgccctgaat tataccaaat aacgcatttt tgttaccaag actgtcaaaa cgctcacggc
    20701 tacgcttcgc ccaacgcaga gacatctcca tagagcgttt tgcgtaatcc cagtcagcag
    20761 gatacggcgt acactcatca aagatcatga cgatatccga accaagatcg tactgaatct
    20821 ccatcgactt ttccgggtcg aggaaaatcg ggtcgccgtt gatcgggtta cggaagtgaa
    20881 cgccctgttc ggtgatttta cgaatatcac caaggctgaa gacctggaag ccgccggagt
    20941 cggtaagaat cggtcctttc cactgcataa aatcgtgcag atcgccgtgc agtttcatga
    21001 tttcctggcc cgggcgcagc cacaggtgga aagtgttgcc gaggataatt tgcgcgccag
    21061 tggcttcaac ttcttccggc gtcatccctt ttacggtgcc gtaggtgcca acaggcataa
    21121 aacaaggcgt ttccactacg ccacgatcaa agaccaggcg gccacggcgt gcgcgaccgt
    21181 cggtggtgtc cagttcaaat ttcatttttt ctccactacg tcagaaaaac agtccaacgt
    21241 ttaaaccagc gccgcggaat tactccccga cgcgctcatt aattgcctgc ggattgtacg
    21301 tgataaacat cgcatcaccg taactaaaaa agcgatattt ctcttctacc gccgctttat
    21361 aggcattcat ggtgtgttga taaccggcaa aggccgaaac cagcataatc agcgtcgact
    21421 ctggcaagtg gaagttcgtc accagcgcat cgaccacttt gtactggaag cccggataga
    21481 taaagatttg ggtatcgtca aagaacggtt cgataagatc gttttttgcc gcctgtgccg
    21541 cgctttccag cgaacgtact gaagtggtgc caaccgcaat cacccggtta ccgcgcgctt
    21601 tcgccgccag taccgcgtct accacatcct gcggtacttc agcgtattcc gagtgcatga
    21661 tgtgatcttc aatggtgtcg acgcgcaccg gctggaaggt gcccgcacca acgtgcaacg
    21721 tcacaaacgc catctccacg cctttggcgc gtaatttttc cagcaaaggc tcgtcaaaat
    21781 gcagaccagc ggtcggggct gcaaccgcgc ccggtttttc gctataaacg gtttgataaa
    21841 gttcgcggtc agcgtcttcg tccggacgat cgatatacgg cggcagcggc atatggccga
    21901 tgctgttgag gatatccagt accgagcgtt catcattaaa ttcgacttca aacagtgcgc
    21961 cgtggcgcgc ggtcattgtt gcgttgatac tttcgtcatc gcccagcaac agttctgcgc
    22021 caggttttgg cgctttcgag gcgcgaatat gcgcaagaat gcgtttgtcg tcgagcatcc
    22081 gctcaaccag cacttcaatc ttgccgccgc tggctttacg cccaaacaga cgcgccggga
    22141 tcacgcgggt attattaaaa accagaagat cgccggggtt gagcttatcg agtaaatcgg
    22201 tgaaagtacc gtgcgtcagc gcacccgtcg gcccgtccag cgacagtaaa cgacagctac
    22261 tgcgttcagg catgggatag tgggcaatca gggattcggg caattcaaag gagaaatcgg
    22321 taacgcgcat gacactgact cagactaaaa taagagtcgg gtagtctagt gccggggcgc
    22381 tctccctgca acctttaccc ccctttggat agaatataat cgatgaattt tttagctcac
    22441 ctgcatttag cccatctcgc ggaaagctcg ctttctggca atttactggc tgatttcgta
    22501 cgcggaaatc ctgaagaaag ttttccgccc gacgtcgtgg ctggcattca tatgcatcga
    22561 cgtattgact gacaatctgc cggaagtccg cgaagcacgg gagtggtttc gtagtgaaac
    22621 gcgccgcgtt gcgcctatta cgctggatgt catgtgggat cactttcttt cccgccactg
    22681 gtcgcagcta tcgccggact ttccgctaca ggaatttgtc tgttatgccc gcgagcaagt
    22741 gatgacgatt ttgccggact caccgccacg ttttatcaat ctgaacaatt acttgtggtc
    22801 agagcagtgg ctggtgcgct atcgcgatat ggatttcatc cagaacgtgt taaacggcat
    22861 ggcaagccgc cgcccacgtc tggatgccct gcgtgactcc tggtacgatt tagacgctca
    22921 ttatgacgcc ctcgaaaccc gcttctggca gttttatccg cggatgatgg cgcaggcgtc
    22981 acacaaggcg ttataaaacg ttagttcatc cataccgtag ccgaaatggc aggcaaagcc
    23041 agaatcccgt cagtcagttg cccatgccct tctttgcatt gccattgcac ggcattgaga
    23101 aacggtgacg cgggtagcac cacttcactg gcctcgccac ggttgattgc caccagtaca
    23161 cgttgctgat tcagcacgcg gacaaatacc actacgttat cttccgcata cagcacctga
    23221 cagccgccac gacgcagcgc ctgacttttc ttacgcagca caatcattcg ccggtacagc
    23281 gcgaataacg ccgtatcctg cttttccacc tgccagggga acggtttacg gcaagacgga
    23341 tcgtttttac catccagtcc cacttcatca ccgtaataaa tgcacggtac accaggccag
    23401 gtgaacagcc agaccaccgc cagcggcagg cgcgcaatat cccgaccgag cagcgtttta
    23461 aatcgcgcag tatcgtggct atcgagctga ttaaacattc gtaattgttg ttgatgagaa
    23521 agccctgcgc ggtaattatc catccaggcc atacaggttt gggcgtcaat ttgctgcgga
    23581 tcataagaga tgtcggtatt ggcaagaaat ccccacaacg ggaatgtgaa gccacgatag
    23641 ttcatggcag catcttccac atcggcctgt aaccattgcc gtgcatcgcc aaaatgttcg
    23701 ccgacaatat acgcttccga ctgggtttct ttcgccgctt cggtgatccc ggcaacgtgc
    23761 tgcatattat tgcgcgcccc acccgcctcc cccagcatat gcaccacatc cagccgccag
    23821 ccgtccatat tccacggcgc tttcagccag tggcggacaa tactgtcttc cccgcgataa
    23881 atttcattca ccagactttc cgactgataa tccagcttcg gcaagctggc atagccaagc
    23941 cagtcgagcg ccgtgccatc atcactaaac aagtaccagt cgcgccaggg cgattcagag
    24001 ttgtgacaag ctccccccgt gccaagattg tgcctgtcaa accaggcatg ggaatcgcca
    24061 ctgtggttaa acacgccgtc cagcaccagc cgcattccca gctgctgcgt attgtgtcgc
    24121 aaacgcagca acgccccatc accgccaaac tgcggatcga catggcgata atcctcggta
    24181 tcgtatttat gtacgctggg agctttaaac accggattga gatacagcgc tgtcacgcca
    24241 agctttttca gatacggcag tttttcgctt atcccgtcca gatcgccgcc atagaacgtt
    24301 gatccgcccg cctgcgccgt gaccggttca tcccagtcac gcaagatgat ctcttgtccg
    24361 gctgcatgat ggtaatagac atgatcctgt tcagcttcac gaggaagact acgcgcaaaa
    24421 cgatcaggga agatctgata aaaaatctga tccgcagccc attgtgggcc gatatccggt
    24481 acatcgacgg caaactgctc cagtcgtgcc ggcggcattc ggctgaagcc ctgcggtgta
    24541 aaccaacgct ggcgatcgtg ccacagcagt ttgaaactgt aacgccgccg gggttgtccg
    24601 ctggagagat caatcgccgc acgccatgcg gtgacgcctg gctgcggctg actgcgctgc
    24661 ttatgcattg gtactgacat ttcttcgtta tcgtgttctg tacgcagcat aatgcgctgc
    24721 ggtgggtctt cgcccgtcag ccacagtgta atgagcagtt gatctttgct ttgtttaaca
    24781 aatgggggca ccggcaggtg ccatgcattt aacatcataa ttcccctgtg atgaaaatct
    24841 gatcaccttg ccatagccta atgcaggatc actcatcatc tgaaggatat ttgggggagg
    24901 aggacggggg cggataactt tcggaatagc cggatcagga tttcctcctg aaccggctta
    24961 cagaacgcaa gtattattgg ttttcagcca gctgacgatc gtggcggcgt ttaaacatcc
    25021 agccaatcaa cagcacaaca atccacgcga aaccgacata cagcgagata cgcgtatccg
    25081 ggtgataacc aatcaacccg ataataaaga gcaggaaaat cagcccgccg atggtcgttg
    25141 ctaccccacc cggcacttta aatttcagcg ccttaacttc ttctggcggc aaacggcgac
    25201 ggaaggcgat ttgcgacagc aggatcataa tccacaccca taccgtggcg aaggttgcca
    25261 gcgaagcgat caccaggaag acgttttccg gcatgatgta gttcagatac accgcaaaca
    25321 gcagcgcggt agtcatcacc agcaccgtaa cccacggaat accgcgacgc gacgttttgc
    25381 tgaaaatttt cggtgcgctg ccctgctctg ccataccgtg gagcatacgg cctacgccaa
    25441 atacgtcact gttaattgcc gacagcgaag cggtcagcac aacaaagtta agaatgctgg
    25501 cggcaaaggt aatgcccata tgctggaacg tcagcacgaa cgggctaccg gcagtgccaa
    25561 cctgattcca cgggtagata gacataatga cgaacagcgt accgacgtag aacaccagaa
    25621 tacgcatcgg cacggagtta atcgcacgcg gaatcgattt ctcaggatct ttcgcttcac
    25681 cggcggtaat cccgataatt tcgatcccac cgtaagcaaa catcaccatt tgcaacgaca
    25741 ttaccatgcc aagccagccg ttactgaaga agccgccgtt gctccacagg ttatgaatac
    25801 cggtcggttg cccgccgttg ccaatccccc agatgatgat gccgaaaccg gcgacaatca
    25861 tgatgatgat ggtggcgact ttaaagaacg agaaccagaa ttccagctca ccgaagacct
    25921 tcacgctcat caggtttacg gcgcaaatga tcagcaccac gctcagtacc caaatccagt
    25981 gcggcaccgt cgggaacaag acacccatat agataccaaa agcggtcaca tcggcgatgg
    26041 cgacaataag gatttcaaag cagtaggtcc atccggtaat gtaacctgcc agcgggccaa
    26101 ggttttcctg cgcataacgc gagaaagagc tggcggccgg gttatgtacc gacatttccc
    26161 ccagcgcacg cataatgata tacgccgcga taccaccgat aatataggcc aacaacacgc
    26221 tcggaccggc cattttgatg gcgtctgccg aaccgtaaaa cagcccggtg ccaattgctg
    26281 aacccagtgc cataaagcgt atgtggcggg tacttagccc acgctttagc ttattcttac
    26341 tttccatcaa ttcctaaccc atcaacacaa taaaaaacca cggggcatcg cagccccgtg
    26401 gttaaaacaa atgttcagtg atttagtgag cgctggaggt cacctgacga cctgccgcgc
    26461 gatcccagat aatggccaga accaccatca ccactgttgg cattaaccac gccagacctt
    26521 gttcggccag cggtaaacgc tgcgcccagg acggtaagat atcgctgaat gcagatgctt
    26581 tgatcccgtc gagaatacca aaaagcaggc tgataaacat cggcggagca atcacgcggg
    26641 acgaattatg ccaccatgag cgtgtaaaac ttaatacaac cagtgcgata cacggcggat
    26701 aaatagcggt cagcaccggt acggagatct ggatcagctg gcttaagccg aggttagaca
    26761 ccaccatcga gaagccgccg aggataaaca ccagcgtacg ataagagagc ggtacgtact
    26821 gggcaaagaa ttctgcacaa gcacaggtca ggccaaccgc cgtgaccaga caggcgatga
    26881 agattaacgc cgccagcagg aagctaccgc cgccgccaaa ggtgtgctga acgtaagcat
    26941 gcagaatagc agcgccgttt gcagactgat cgaccagcga cgcgctgtct gaacccagac
    27001 agaacagcgc caggtacagc aaagtcagac caacacccgc catcaggcca gcccagacgg
    27061 tataacgggt cagcagacgc gcttcggtaa cgccacgaga acgcgccgcg ttaacaataa
    27121 cgataccaaa caccattgcg cccagcgtat ccatggtcag atagccgtta acgaagccgt
    27181 tagaaaatgc agcgttttga taagcctcag tcgccgtgct gatagaaccc gccggccaga
    27241 caatcgcgac aacagacagg atgaccagcg cgataatttt cagcggcgca aggaagttgc
    27301 ccacggtatc cagcagcttg cccggataga gcgaaaccag aataacgata gcgaaataga
    27361 ccaggctgta gataaacagc ggcagcgcgg aatcacccgt cagcggcgca atccccactt
    27421 caaaggaaac ggtagctgta cgcggcgtag cgaaaagcgg ccccaccgcc aggtaacaaa
    27481 ccgttgccag cagtacgcca gcgactttac cgattggcgt gctgaggctg tcaacaccgc
    27541 cgccaacttt tgccagcgcc actaccgtta ataccggcag gccaacggca gtaatgagga
    27601 agccgaatgc cgcagtccag acgtgttcgc ctgcctgtaa gccgaccatt ggagggaaaa
    27661 taatgttacc tgcgccgacg aacaacgcaa atgtcataaa gcccagagcg atgatatcgc
    27721 gcgatcttaa ttgatgggtc ataaatcata ctgcctgtgg atgtggtgtt gtaacgttaa
    27781 atttttgtgc caatccctgg ggatgataca gcggtatatt taaacaattt tagcgggaaa
    27841 tactcccaac caggcgcggc gaagaatagt ttttcgattt accgcgttaa tgcagtcagt
    27901 atgacagcac ctgaagcgca atttaaacgc ttataacgtt ttaaagcaag gagggatcta
    27961 aaaaccagag gtatatagcg ataagaaatc accaaccagt ataaaacact atttccgtga
    28021 aaaagacatg gctaatcatg cgaacaaaaa atcaccaacc gttgtaaaat ctaacgcccg
    28081 tattttgact gcaaagaaaa taagccagca tcggctggct tatggcaata aaagatgaca
    28141 aaggcggatt aatcgctgtt tttggcaatt aaacgttccg ggataacaaa actgaaacgt
    28201 gttccttttc ctactgtact ctcaatattc aggcgacttt cgtgatgatt caccgcatgt
    28261 ttcacgatcg ctaaccctaa tccgctaccg ccggtttgcc gggaacgcgc tttatcaacg
    28321 cgataaaaac gctcggtcag gcgcggaata tgctccggtg caatgcccgg tccgttatct
    28381 tcaacgctaa attcggcacc gtgcggcact cgctgccagc gtacggtgat atgcgtgcct
    28441 tccggcgtat gattcacggc gttatagacc aggttcgaaa tcgcactgcg tagctgatct
    28501 tcgttgccag acaccttgag gccgttatct atctcaaagg taaatgtctg ttttttctga
    28561 ctcagagtct gagcctcgcg ctcaacaacg cgcagcatca tcggcacatc aaccttttca
    28621 ttgagcaatt gcgtcggcga ggcttcaatt ttcgacagcg ttagcaactg cttcaccagc
    28681 ccttccatcc gctgggtctg ctcgcgcatg gtgtgcaacg ctttttcgcg taccgcgcct
    28741 tccagcggct gctcatccat catctccagg taaccctgta acacggtcaa tggcgtacgt
    28801 aactcatggc tcacgttggc aaaaaagtta cgccgcgccc cttccagttg atgcatttgc
    28861 gtgacatcac gcgccaccat caacaactgt ttgtgggtat aaggcatgac gcgaatttcc
    28921 agatgccgcc cggtgttgaa caccagattg agcgggcgag aaaaatcacg cgttttcaga
    28981 tattgcgtaa actccgggta acgcagtagg ttaaggatgt tctgcccgtt atcttccggc
    29041 cagcgcaaac caagaatttg ttgcgccaga ccgttacacc agaaaatacc gccctcttcc
    29101 gtggtcagca ccaccgcgtc gggcagcgac tccgcgccgc tacgaaagcg tttaatcaga
    29161 ttgcccagtt cacggcggcg ttttttattt cgcagctgca tctggtgtaa gccgtatagt
    29221 agcggttccc agctaccacg ccccggtggc ggggtcatac tgcgatccac ccacagccac
    29281 catgaaaggc gcaataaatt ccagaaatgc cagataagca gtcctgttac cgatgccagc
    29341 aaaaaccagg gcaggtaacc aaaaaatgca cccaggatga aagccgggag gcagcaaagt
    29401 agcagctcca gcaccagcct tttccacgac agccgttcca gcacgtaaga tactccagtt
    29461 aagaaatcat aagccctgct ctgcgtccga tgagcaaggc gttaaaagcg ggttgaaaaa
    29521 cgatatcctg taccgcgcac ggtctgcacc atgcggtcat gcccgccggg ctccagtgct
    29581 ttacgcaggc gacgaatgtg gacatcgacc gtgcggtctt ccacatacac gttagttccc
    29641 cagacgtggt ttaacagctg ctcgcggctg tacacgcgct caggatgcgt cataaaaaag
    29701 tgcagcagtt taaattctgt cggccccatc tccagcggct cttcgcccgc catcactcgg
    29761 tgagatgtcg ggtcgagact taatccctgc atctcaatca cctcttccac cgccattggc
    29821 gaaatacggc gcattaccgc tttgattcgc gccaccagct ccttcggcga aaatggcttg
    29881 gtgatatagt catccgcgcc ggtttcaagg ccgcgcacgc gatcttcttc ttcccctctg
    29941 gcggtcaaca tcaccactgg aatatcccgg gtcatcgact cgcgcttgag gtgtttgatg
    30001 aactggatac cggagccgcc aagtaacatc cagtcaagga gaattaaatc cggccaaggt
    30061 tcattcagtt gattcacagc actgtcataa tcttccgctt cgaccggctg aaagccattt
    30121 tgttcgagca cgaagcagac catttcgcga attggagctt catcttctac gaccagaata
    30181 cgtctcgcca tgatttgccc tgttgtaata aataggttgc gatcattaat gcgacgtcat
    30241 tatgcgtcag atttatgaca gatttatgaa aagctcgtcg cacatatctt caggttattg
    30301 atttccgtgg cgcagaaaaa agcaaatggc acatctgttt gggtataatc gcgaccatgc
    30361 tttttcgcca gggaaccgtt atgcgcatcc ttcacacctc agactggcat ctcggccaga
    30421 acttctacag taaaagccgc gaagctgaac atcaggcttt tcttgactgg ctgctggaga
    30481 cagcacaaac ccatcaggtg gatgcgatta ttgttgctgg tgatgttttc gataccggct
    30541 cgccgcccag ttacgcccgc acgttataca accgttttgt tgtcaattta cagcaaactg
    30601 gctgtcatct ggtggtactg gcaggaaacc atgactcggt cgccacgctg aatgaatcgc
    30661 gcgatatcat ggcgttcctc aatacaaccg tggtcgccag cgccggacat gcgccgcaaa
    30721 tcttgcctcg tcgcgacggg acgccaggcg cagtgctgtg ccccattccg tttttacgcc
    30781 cgcgtgacat tatttccagc caggcggggc ttaacggtat tgaaaaacag cagcatttac
    30841 tggcagcgat taccgattat taccaacaac actatgccga tgcctgcaaa ctgcgcggcg
    30901 atcagcctct gcccatcatc gccacgggac atttaacgac cgtgggtgcc agtaaaagtg
    30961 acgccgtgcg tgacatttat attggcacgc tggacgcgtt tccggcacaa aactttccac
    31021 cagccgacta catcgcgctc gggcatattc accgcgcaca gattattggc ggcatggaac
    31081 atgttcgcta ttgcggttcc cccattccac tgagttttga tgaatgcggt aagagtaaat
    31141 atgtccatct ggtgacattt tcaaacggca aattagagag cgtggaaaac ctgaacgtac
    31201 cggtaactca acccatggcg gtgctgaaag gcgatctggc gtcgattacc gcacagctgg
    31261 aacagtggcg cgatgtatcg caggagccac ctgtctggct ggatatcgaa atcactactg
    31321 atgagtatct gcatgatatt cagcgcaaaa tccaggcatt aaccgaatca ttgcctgtcg
    31381 aagtattgct ggtacgtcgg agtcgtgaac agcgcgagcg tgtgttagcc agccaacagc
    31441 gtgaaaccct cagcgaactc agcgtcgaag aggtgttcaa tcgccgtctg gcactggaag
    31501 aactggatga atcgcagcag caacgtctgc agcatctttt caccacgacg ttgcataccc
    31561 tcgccggaga acacgaagca tgaaaattct cagcctgcgc ctgaaaaacc tgaactcatt
    31621 aaaaggcgaa tggaagattg atttcacccg cgagccgttc gccagcaacg ggctgtttgc
    31681 cattaccggc ccaaccggtg cggggaaaac caccctgctg gacgccattt gtctggcgct
    31741 gtatcacgaa actccgcgtc tctctaacgt ttcacaatcg caaaatgatc tcatgacccg
    31801 cgataccgct gaatgtctgg cggaggtgga gtttgaagtg aaaggtgaag cgtatcgcgc
    31861 gttctggagc cagaatcggg cgcgtaacca gccggacggt aatttgcagg tgcctcgcgt
    31921 agagctggcg cgctgcgccg acggcaaaat tctcgccgac aaagtgaaag ataagctgga
    31981 actgacagcg acgttaaccg ggctggatta cgggcgcttc acccgttcga tgctgctttc
    32041 gcaggggcaa tttgctgcct tcctgaatgc caaacccaaa gaacgcgcgg aattgctcga
    32101 ggagttaacc ggcactgaaa tctacgggca aatctcggcg atggtttttg agcagcacaa
    32161 atcggcccgc actgagctgg agaagctgca agcgcaggcc agcggcgtca cgttgctcac
    32221 gccggaacaa gtgcaatcgc tgacagcgag tttgcaggta cttactgacg aagaaaaaca
    32281 gttaattacc gcgcagcagc aagaacaaca atcgctaaac tggttaacgc gtcaggacga
    32341 attgcagcaa gaagccagcc gccgtcagca ggccttgcaa caggcgttag ccgaagaaga
    32401 aaaagcgcaa cctcaactgg cggcgcttag tctggcacaa ccggcacgaa atcttcgtcc
    32461 acactgggaa cgcatcgcag aacacagcgc ggcgctggcg catattcgcc agcagattga
    32521 agaagtaaat actcgcttac agagcacaat ggcgcttcgc gcgagcattc gccaccacgc
    32581 ggcgaagcag tcagcagaat tacagcagca gcaacaaagc ctgaatacct ggttacagga
    32641 acacgaccgc ttccgtcagt ggaacaacga actggcgggt tggcgtgcgc agttctccca
    32701 acaaaccagc gatcgcgagc atctgcggca atggcagcaa cagttaaccc atgctgagca
    32761 aaaacttaat gcgcttgcgg cgatcacgtt gacgttaacc gccgatgaag ttgctaccgc
    32821 cctggcgcaa catgccgagc aacgcccact gcgtcagcgc ctggtcgcgc tgcatgggca
    32881 gattgttccc caacaaaaac gtctggcgca gttaatggtc actatccaga atgtcactct
    32941 ggaacagacg caacgtaatg tcgcacttaa cgaaatgcgc cagcgttata aagaaaagac
    33001 gcagcaactt gccgatatga aaaccatttg cgagcaggaa gcgcgcatca aaacgctgga
    33061 agcccagcgt gcacagttac aggcgggtca gccttgtcca ctttgtggtt ccaccagcca
    33121 cccggcggtc gaggcgtatc aggcgctgga gcctggtgtt aatcagtctc gattactggc
    33181 gctggaaaac gaagttaaaa agctcggcga agaaggtgcg gcgctgcgcg ggcaactgga
    33241 tgcattaacg aagcagcttc agcgcgatga aaacgaagcg caaagcctcc gacaagatga
    33301 gcaagcactt actcaacaat ggcaagccgt cacggccagc ctcaatatca ccttgcagcc
    33361 acaggacgat attcaaccgt ggctggatgc acaagatgag cacgaacgcc agctgcggtt
    33421 actcagccaa cgacatgaat tacaagggca gattgccgcg cataatcagc aaattatcca
    33481 gtatcaacag caaattgaac aacgtcagca gcagctctta acggcattgg cgggttatgc
    33541 actgacattg ccacaggaag atgaagaaga gagctggttg gcgacacgtc agcaagaagc
    33601 gcagagctgg cagcaacgcc agaacgaatt aaccgcgctg caaaaccgta ttcagcagct
    33661 gacgccgatt ctggaaacgt tgccgcaaag tgatgatctc ccgcacagcg aagaaactgt
    33721 ggagctggat aactggcggc aggttcatga acaatgtctc gcattacaca gccagcagca
    33781 gacgttacag caacaggatg ttctggcggc gcaaagtctg caaaaagccc aagcgcagtt
    33841 tgacaccacg ctacaggcca gcgtctttga cgatcagcag gcgttccttg cggcgctaat
    33901 ggatgaacaa acactaacgc agctggaaca gctcaagcag aatctggaaa accagcgccg
    33961 tcaggcgcaa acgctggtca ctcagacagc agaaacgctg gcacagcatc aacaacaccg
    34021 acctgacggg ttggctctca ctgtgacggt ggagcagatt cagcaagagt tagcgcaaac
    34081 tcaccaaaag ttgcgtgaaa acaccacgag tcaaggcgag attcgccagc agctgaagca
    34141 ggatgcagat aaccgtcagc aacaacaaac cttaatgcag caaattgctc aaatgacgca
    34201 gcaggttgag gactggggat atctgaattc gctaataggt tccaaagagg gcgataaatt
    34261 ccgcaagttt gcccaggggc tgacgctgga taatttagtc catctcgcta atcagcaact
    34321 tacccggctg cacgggcgct atctgttaca gcgcaaagcc agcgaggcgc tggaagtcga
    34381 ggttgttgat acctggcagg cagatgcggt acgcgatacc cgtacccttt ccggcggcga
    34441 aagtttcctc gttagtctgg cgctggcgct ggcgctttcg gatctggtca gccataaaac
    34501 acgtattgac tcgctgttcc ttgatgaagg ttttggcacg ctggatagcg aaacgctgga
    34561 taccgccctt gatgcgctgg atgccctgaa cgccagtggc aaaaccatcg gtgtgattag
    34621 ccacgtagaa gcgatgaaag agcgtattcc ggtgcagatc aaagtgaaaa agatcaacgg
    34681 cctgggctac agcaaactgg aaagtacgtt tgcagtgaaa taactattca gcaggataat
    34741 gaatacagag gggcgaatta tctcttggcc ttgctggtcg ttatcctgca agctatcact
    34801 ttattggcta cggtgattgg tagccgttct ggtggttgtg atggtggtat gaaaaaagtc
    34861 attttatctt tggctctggg cacgtttggt ttggggatag ccgaatttgg cattatgagc
    34921 gtgctcacgg agctggcgca taacgtagga atttcgattc ctgctgccgg gcatatgatc
    34981 tcgtattatg cactggtggt ggtggtcggt gcgccaatca tcgcactctt ttccagccgc
    35041 tacttactca aacatatctt gttgtttctg gtggcgttgt gcgtcattgg caacgccatg
    35101 ttcacgctct cttcgtctta cctgatgctc gccattggtc ggctggtatc cggctttccg
    35161 catggcgcat tttttggcgt cggagcgatc gtgttatcaa aaattatcaa acccggaaaa
    35221 gtcaccgccg ccgtggcggg gatggtttcc gggatgacag tcgccaattt gctgggcatt
    35281 ccgctgggaa cgtatttaag tcaggaatgc tggcgttaca cctttttatt gatcgctgtt
    35341 tttaatattg cggtgatggc atcggtctat ttttgggtgc cagatattcg cgacgaggcg
    35401 aaaggaaatc tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg gttaattttc
    35461 gccgccacga tgtttggcaa cgcaggtgtg tttgcctggt tcagctacgt aaagccatac
    35521 atgatgttta tttccggttt ttcggaaacg gcgatgacct ttattatgat gttagttggg
    35581 ctagggatgg tgctgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg
    35641 cgcattgcag cagtgactga ctttatcatt gtactggcac tgctgatgct ctttttctgc
    35701 ggcggcatga aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc
    35761 ctttcagcac ctctgcaaat attgttactg caaaatgcca aaggcggaga gttattaggt
    35821 gccgcaggtg ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggaggt
    35881 atgatgctga cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgttttcg
    35941 tttgctgcga tgtcatcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat
    36001 agtccggtgc tggcgaaacc actggggtag attactgtca cggtgcgcgt tgcttattgc
    36061 cggatgcggc gtgaacgcct tatccgccct acgcggttct ggcacatttt gtaggcctga
    36121 taagacgcgg caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt ttttactctt
    36181 gtggccataa ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc gccttactca
    36241 ccggcgtttc acattcgccg ccgaagacaa attgtttaat caactgccca accgtttgat
    36301 ataaacggtc tacattgctc atcccgcccc ccaggacaat cacatccgga tcgagaatat
    36361 tcacgacatg tgccagcgat tttgccagcc gcagctcgta gcgacgcaat gccagttccg
    36421 ctaccggatc gctttcttca accaggcgga taatttcact gcctttcagc gcatgtccgc
    36481 tcaaacgacg ataatccgtc gcgaatcccg tgcccgaaat aaaggtttca atacaacctt
    36541 gtttaccgca ataacaaggg acttcctcgc gataacgcag ttcgtcttcg tccatccacg
    36601 gtagcggatt gtgtccccac tcacctgccg tgccattgcc gccgatatgc gtccgcccat
    36661 tgaatgccac gcccgcgccg catcccgtgc cgataatcac ggcaaatacc gtctgcgctc
    36721 ccgctgccgc gccatctact gcttctgaaa ccgccagaca gttggcatca tttgccagcc
    36781 gcacttcccg ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg
    36841 ttgaattggc attcttcacc acaccggtgt agggcgaaat cgaacctgga atgcccatac
    36901 ctaccgttcc gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt
    36961 caatagtctg ccggtaatca tcacgcggcg tgggcagacg atggcggtac aactgctccc
    37021 ctgcatcgcc cagtgcaatc acttcagttt tggtgccgcc taaatcgata cctatacgca
    37081 ctgtactctc cttatttttt tcaatatcaa tagcgtagag acggacaacc ggattggcaa
    37141 tgcaaggccg cctacaattc gttatcatgc ccgctaaatt taacgacaag gccgtggaaa
    37201 ttatcatgct gtggttcaaa aatttaatgg tttaccgtct tagccgcgag atttcgctgc
    37261 gtgcagaaga gatggaaaaa cagctagcct cgatggcatt taccccatgc agcagccagg
    37321 acatggcgaa gatgggctgg gttcctccga tgggatcgca cagcgatgcg ttaacgcacg
    37381 ttgccaatgg tcaaattgtt atctgcgcgc gcaaagaaga aaaaatcctc ccgtctccgg
    37441 tgattaaaca ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc
    37501 tgaagaaaac cgaaaaagat tcgctgaaag acgaagtgct gcactctctg ctgccgcgtg
    37561 ctttcagccg ttttagccag acaatgatgt ggctcgacac ggttaacggt ttgattatgg
    37621 tggactgcgc cagtgccaaa aaagcggaag atacgctggc attactgcgt aaaagcctgg
    37681 ggtcgttacc ggttgtacca ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat
    37741 gggttcgctc cggtagtgcg gcacagggct tccagctgct tgatgaagcc gagctgaaat
    37801 cgttgctgga agatggcggc gtgatccgcg cgaagaaaca agatctgacc agcgaagaga
    37861 tcaccaatca cattgaagcc ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc
    37921 gcattcagtt tgtgatgtgc gacgatggtt cgctcaagcg tcttaagttc tgcgacgagc
    37981 tgcacgatca aaacgaagat atcgaccgtg aagatttcgc ccagcgtttt gatgccgatt
    38041 tcatcctgat gactggtgaa ctggcagcgt taattcaaaa cctgattgaa ggattaggtg
    38101 gcgaagcaca acgttaattg ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc
    38161 ttatccggcg tacgaagcca caccaggcat ataattattc gctacggcga gcaataattt
    38221 ttagcgcagc aatattatgc gttttacgcc gtaactgatc taatcgcctc atccctattc
    38281 gcctgccagc agcacgcaga atattcacta acccccattt atcgctggta aattgttttg
    38341 ttaacagctc cgcttttggg gtatcaatat attcacgcaa taaatcagaa attgaacaaa
    38401 tatccgtcag gtaaggatac cagcaagttt caccgtgaaa attacgcact ccagcgttac
    38461 ctgcaataaa gtctttcggg taatcccaga tcacgttttt atccaacgtg atccaataac
    38521 gcggtaaatc ggtacttcca ccattttggc tgcgcatggg ataacgggta cagtgaattt
    38581 gcaggttgat cgttggcgtc agtaagtcat agatctcccg ctgtaacttg ctccatggac
    38641 gttgtgtcat tgtttttcct caagccggtg acgcgcttca ctgcgaattt tccgcgttgc
    38701 cccggttttc actaaatcac gtaactgcga aagatcgggt tgttcgacaa ataagagtaa
    38761 tgatggatcc tcttttaacc acgccgtttt cacatcggca gccagctgtg gattattaat
    38821 agccagcgac cgatcctgag gctcagtcaa tgtcgttaac aacgacgccg gggtatgtgc
    38881 gttgcgtaat gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca
    38941 ttcatcatct gatttatctt ttatcgtagc cagttccgca aaaaatacct gga
//
LOCUS       DADMEQ010000022        38743 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.022, whole genome shotgun sequence.
ACCESSION   DADMEQ010000022 DADMEQ010000000
VERSION     DADMEQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.022"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="JNZ05_001221"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="JNZ05_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HAY8564523.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            530..580
                     /gene="hisL"
                     /locus_tag="JNZ05_001222"
     CDS             530..580
                     /gene="hisL"
                     /locus_tag="JNZ05_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HAY8564524.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            726..1625
                     /gene="hisG"
                     /locus_tag="JNZ05_001223"
     CDS             726..1625
                     /gene="hisG"
                     /locus_tag="JNZ05_001223"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAY8564525.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1631..2935
                     /gene="hisD"
                     /locus_tag="JNZ05_001224"
     CDS             1631..2935
                     /gene="hisD"
                     /locus_tag="JNZ05_001224"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAY8564526.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2932..4002
                     /gene="hisC"
                     /locus_tag="JNZ05_001225"
     CDS             2932..4002
                     /gene="hisC"
                     /locus_tag="JNZ05_001225"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAY8564527.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            4002..5069
                     /gene="hisB"
                     /locus_tag="JNZ05_001226"
     CDS             4002..5069
                     /gene="hisB"
                     /locus_tag="JNZ05_001226"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HAY8564528.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KEGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5069..5659
                     /gene="hisH"
                     /locus_tag="JNZ05_001227"
     CDS             5069..5659
                     /gene="hisH"
                     /locus_tag="JNZ05_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAY8564529.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5659..6396
                     /gene="hisA"
                     /locus_tag="JNZ05_001228"
     CDS             5659..6396
                     /gene="hisA"
                     /locus_tag="JNZ05_001228"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAY8564530.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGISLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6378..7154
                     /gene="hisF"
                     /locus_tag="JNZ05_001229"
     CDS             6378..7154
                     /gene="hisF"
                     /locus_tag="JNZ05_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAY8564531.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7148..7759
                     /locus_tag="JNZ05_001230"
     CDS             7148..7759
                     /locus_tag="JNZ05_001230"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HAY8564532.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7945..8922)
                     /gene="wzzB"
                     /locus_tag="JNZ05_001231"
     CDS             complement(7945..8922)
                     /gene="wzzB"
                     /locus_tag="JNZ05_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HAY8564533.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9068..10234)
                     /gene="ugd"
                     /locus_tag="JNZ05_001232"
     CDS             complement(9068..10234)
                     /gene="ugd"
                     /locus_tag="JNZ05_001232"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HAY8564534.1"
                     /translation="MKITISGTSYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRTSSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(10484..11890)
                     /gene="gndA"
                     /locus_tag="JNZ05_001233"
     CDS             complement(10484..11890)
                     /gene="gndA"
                     /locus_tag="JNZ05_001233"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAY8564535.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11999..12358)
                     /locus_tag="JNZ05_001234"
     CDS             complement(11999..12358)
                     /locus_tag="JNZ05_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564536.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12355..12906)
                     /locus_tag="JNZ05_001235"
     CDS             complement(12355..12906)
                     /locus_tag="JNZ05_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564537.1"
                     /translation="MIAKKRNIQTFLIFSLATILGGIYWYVRSFWISGDPFSPAGGNI
                     FGHYLWNEIDLQVQTAEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKIL
                     WVFFSIVLTYVLFWFSVTQGDRYLSPIYPLSVLLVAISIASLIKDINITNYKLGRYFI
                     LVCIIFFRGDRCILNSNIFIMNT"
     gene            complement(13007..13606)
                     /locus_tag="JNZ05_001236"
     CDS             complement(13007..13606)
                     /locus_tag="JNZ05_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564538.1"
                     /translation="MSLNILIIYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLA
                     INNIEFSTLFVILFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYL
                     RFPLFPQNADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPSIIAT
                     ILLFTINPLKDHLGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13778..14716)
                     /locus_tag="JNZ05_001237"
                     /pseudo
     CDS             complement(13778..14716)
                     /locus_tag="JNZ05_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14779..15153)
                     /locus_tag="JNZ05_001238"
     CDS             complement(14779..15153)
                     /locus_tag="JNZ05_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HAY8564539.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15157..16305)
                     /gene="wzy"
                     /locus_tag="JNZ05_001239"
     CDS             complement(15157..16305)
                     /gene="wzy"
                     /locus_tag="JNZ05_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HAY8564540.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFLCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16325..17194)
                     /locus_tag="JNZ05_001240"
     CDS             complement(16325..17194)
                     /locus_tag="JNZ05_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAY8564541.1"
                     /translation="MATYNGECWIEEQLKSIIEQKDVDISIFISDDLSTDNTLNICEE
                     FQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQDDIWYKDKIKNAI
                     DCLVFNNANCYSSNVIAYYPSGQKNLVDKAQSQTQFDYFFEAAGPGCTYVIKKETLIE
                     FKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQHENNQVGANISFK
                     AKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILALSFWKLRRKKAD
                     KIYILLMLILNIF"
     gene            complement(17223..18113)
                     /locus_tag="JNZ05_001241"
     CDS             complement(17223..18113)
                     /locus_tag="JNZ05_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HAY8564542.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18143..19399)
                     /gene="wzx"
                     /locus_tag="JNZ05_001242"
     CDS             complement(18143..19399)
                     /gene="wzx"
                     /locus_tag="JNZ05_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HAY8564543.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19415..19960)
                     /gene="rfbC"
                     /locus_tag="JNZ05_001243"
     CDS             complement(19415..19960)
                     /gene="rfbC"
                     /locus_tag="JNZ05_001243"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAY8564544.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19965..20843)
                     /gene="rfbA"
                     /locus_tag="JNZ05_001244"
     CDS             complement(19965..20843)
                     /gene="rfbA"
                     /locus_tag="JNZ05_001244"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY8564545.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20902..21801)
                     /gene="rfbD"
                     /locus_tag="JNZ05_001245"
     CDS             complement(20902..21801)
                     /gene="rfbD"
                     /locus_tag="JNZ05_001245"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAY8564546.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21801..22886)
                     /gene="rfbB"
                     /locus_tag="JNZ05_001246"
     CDS             complement(21801..22886)
                     /gene="rfbB"
                     /locus_tag="JNZ05_001246"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY8564547.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(23259..24152)
                     /gene="galF"
                     /locus_tag="JNZ05_001247"
     CDS             complement(23259..24152)
                     /gene="galF"
                     /locus_tag="JNZ05_001247"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HAY8564548.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24327..25721)
                     /gene="wcaM"
                     /locus_tag="JNZ05_001248"
     CDS             complement(24327..25721)
                     /gene="wcaM"
                     /locus_tag="JNZ05_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HAY8564549.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLNHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFIRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25732..26952)
                     /gene="wcaL"
                     /locus_tag="JNZ05_001249"
     CDS             complement(25732..26952)
                     /gene="wcaL"
                     /locus_tag="JNZ05_001249"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HAY8564550.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26949..28229)
                     /gene="wcaK"
                     /locus_tag="JNZ05_001250"
     CDS             complement(26949..28229)
                     /gene="wcaK"
                     /locus_tag="JNZ05_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HAY8564551.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28409..29887)
                     /gene="wzxC"
                     /locus_tag="JNZ05_001251"
     CDS             complement(28409..29887)
                     /gene="wzxC"
                     /locus_tag="JNZ05_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-antigen undecaprenyl disphosphate flippase"
                     /protein_id="HAY8564552.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29889..31283)
                     /gene="wcaJ"
                     /locus_tag="JNZ05_001252"
     CDS             complement(29889..31283)
                     /gene="wcaJ"
                     /locus_tag="JNZ05_001252"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HAY8564553.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLAHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGGNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31338..32708)
                     /gene="cpsG"
                     /locus_tag="JNZ05_001253"
     CDS             complement(31338..32708)
                     /gene="cpsG"
                     /locus_tag="JNZ05_001253"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HAY8564554.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32813..34249)
                     /gene="cpsB"
                     /locus_tag="JNZ05_001254"
     CDS             complement(32813..34249)
                     /gene="cpsB"
                     /locus_tag="JNZ05_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HAY8564555.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADKDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34252..35464)
                     /gene="wcaI"
                     /locus_tag="JNZ05_001255"
                     /pseudo
     CDS             complement(34252..35464)
                     /gene="wcaI"
                     /locus_tag="JNZ05_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
     gene            complement(35461..35940)
                     /locus_tag="JNZ05_001256"
     CDS             complement(35461..35940)
                     /locus_tag="JNZ05_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HAY8564556.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35940..36908)
                     /gene="fcl"
                     /locus_tag="JNZ05_001257"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35940..36908)
                     /gene="fcl"
                     /locus_tag="JNZ05_001257"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAY8564557.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENSQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36911..38032)
                     /gene="gmd"
                     /locus_tag="JNZ05_001258"
     CDS             complement(36911..38032)
                     /gene="gmd"
                     /locus_tag="JNZ05_001258"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAY8564558.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38058..38606)
                     /gene="wcaF"
                     /locus_tag="JNZ05_001259"
     CDS             complement(38058..38606)
                     /gene="wcaF"
                     /locus_tag="JNZ05_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HAY8564559.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38622..>38743)
                     /locus_tag="JNZ05_001260"
     CDS             complement(38622..>38743)
                     /locus_tag="JNZ05_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyl transferase"
                     /protein_id="HAY8564560.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10344 a         9693 c         8719 g         9987 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgag ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcaccatca tcctgactag tctttcaggc gatgtgtgct
      601 ggaagacatt cagatcttcc agtggtgcat gaacgcatga gaaagccccc ggaagatcac
      661 cttccggggg cttttttatt gcgcggttgt taacggttca gacaggttta aagaggaata
      721 agaaaatgac agacaacact cgtttacgca tagctatgca gaaatccggc cgtttaagtg
      781 atgactcacg cgaattactg gcgcgctgtg gcattaaaat caatcttcac acccagcgcc
      841 tgatcgcgat ggcagaaaac atgccgattg atattctgcg cgtgcgtgac gatgacattc
      901 ccggtctggt aatggacggc gtggtagacc ttgggattat cggcgaaaac gtgctggaag
      961 aagagctgct taaccgccgc gcccagggtg aagatccgcg ctactttacc ctgcgtcgtc
     1021 tggatttcgg cggctgccgc ctttcgctgg caacgccggt tgatgaagcc tgggacggtc
     1081 cgctctcctt aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc aagcgttatc
     1141 tcgaccagaa aggtatctct tttaaatcct gcttactgaa cggttctgtc gaagttgcgc
     1201 cgcgtgccgg gctggcggat gcgatttgcg atctggtttc caccggtgcc acgctggaag
     1261 ctaacggcct gcgcgaagtc gaagttatct accgctcgaa agcctgcctg atccagcgcg
     1321 atggcgaaat ggaagaatct aaacagcaac tgatcgacaa gctactgacc cgtattcagg
     1381 gtgtgatcca ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc gaacgcctag
     1441 atgaagtcat cgccctgctg ccaggtgccg aacgcccaac cattctgccg ctggcgggtg
     1501 atcaacagcg cgtagcgatg cacatggtga gtagcgaaac cctgttctgg gaaacgatgg
     1561 aaaaactgaa agcactgggt gccagttcaa tcctggtcct gccgattgag aagatgatgg
     1621 agtgatcgcc atgagcttta acacaatcat tgactggaat agctgtactg cggagcaaca
     1681 acgccagctg ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc gcaccgttaa
     1741 cgatattctc gataacgtga aaacgcgtgg cgatgaggcc ctgcgtgaat acagcgcgaa
     1801 gtttgataaa accacggtta ccgcgctgaa ggtgtctgct gatgagatcg ccgccgccag
     1861 cgaacgcctg agcgacgagc taaaacaggc gatggcggtg gcagtaaaga gtattgaaac
     1921 cttccacact gcgcaaaaac tgccgccggt agatgtagaa acgcagccag gcgtgcgttg
     1981 ccagcaggtc acgcgcccgg tggcttcagt gggtttgtat attccgggcg gctccgcccc
     2041 gctcttctca acggtattaa tgctggcaac tccggcgcgt attgcgggct gtaaaaaagt
     2101 ggtgctgtgc tcaccgccgc cgattgccga tgaaattctt tatgcggcgc agctgtgcga
     2161 tgtgcaggac gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg cgtttggtac
     2221 ggaatctgtg ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg tcaccgaagc
     2281 aaaacgccag gtaagccagc gtctggacgg tgcggcgatc gatatgcccg caggcccgtc
     2341 ggaagtgctg gtgattgctg acagcggcgc tacgccggat tttgtggctt ctgatttgct
     2401 ttctcaggct gaacacggcc ctgactcaca ggtaatttta ctgacccccg atgctgatat
     2461 ggcgcgtcgc gttgccgagg ccgtcgaacg ccaactggca gaactgccgc gtgccgaaac
     2521 cacccgccag gcactgaacg ccagccgcct gatcgtgact aaagatttag cgcagtgcgt
     2581 ggagatctcc aaccagtacg gcccggagca cctgatcatt cagacccgca acgcccgcga
     2641 actggtcgat ggcatcacca gcgccggttc ggtatttctt ggtgactggt caccggaatc
     2701 ggcaggcgac tacgcctccg gcaccaacca cgtgctgccg acttacggtt acaccgccac
     2761 ctgttccagc ctcgggctgg cagatttcca gaaacgcatg accgtacagg aactgtcgaa
     2821 agaaggtttc tccgcgctgg cttcgaccat tgaaacactg gccgccgccg agcgcctgac
     2881 cgcccacaaa aatgccgtta ctttgcgtgt taacgtcctt aaggagcaag catgagcacc
     2941 gtgactatta ctgatttagc gcgtgaaaac gtccgcaacc tgacgccgta tcagtcggcg
     3001 cgtcgtctgg gcggtaacgg cgacgtctgg ctgaacgcca acgaatatcc cacagccgtg
     3061 gagtttaagc ttactcagca aacgctcaac cgctacccgg aatgccagcc aaaagcggtg
     3121 attgaaaatt acgcgcaata tgcaggcgta aaaccggaac aggtgctggt cagccgtggt
     3181 gcggacgaag gtattgaact gctgatccgc gctttttgcg aaccgggtaa agacgccatc
     3241 ctctactgcc cgccaacgta cggcatgtac agcgtcagcg ctgaaaccat tggtgtcgaa
     3301 tgccgcacag tgccgacgct ggaaaactgg caactggact tacagggcat ttccgacaag
     3361 ctggacggcg taaaagtggt ctatgtttgc agccccaaca accccaccgg gcaactgatc
     3421 aatccgcagg attttcgcac cctgctggag ctaacccgcg gtaaggcgat tgtggttgcc
     3481 gatgaagctt atatcgagtt ttgcccgcag gcatcgttag ctggctggct ggcggaatat
     3541 ccgcacctgg cgattttgcg taccctttcg aaagccttcg cgttggcggg gcttcgttgc
     3601 ggatttacgc tggcaaacga agaagtcatc aacctgctga tgaaagtgat ccccccctac
     3661 ccgctctcga cgccggttgc cgacattgcc gcacaggcgt taagcccgca gggaatcgtc
     3721 gccatgcgcg aacgggtggc gcaaattatt acagaacgcg aatacctgat tgccgcactg
     3781 aaagagatcc cctgcgtgga gcaggttttc gactctgaaa ccaactacat tctggcgcgc
     3841 tttaaagcct ccagtgcagt gtttaaatct ttgtgggatc agggcattat cttacgtgat
     3901 cagaataaac aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa
     3961 agccagcgcg tcattgacgc cttacgtgcg gagcaagttt gatgagtcag aagtatcttt
     4021 ttatcgatcg cgatggaacc ctgattagcg aaccgccgag tgattttcag gtggaccgtt
     4081 ttgacaaact cgcctttgaa ccgggcgtga tcccggaatt gctgaagctg caaaaagagg
     4141 gctacaagct ggtgatgatc actaatcagg atggtctggg aacacaaagt ttcccgcaag
     4201 cggattttga tggcccgcac aacctgatga tgcagatctt cacctcgcaa ggcgtgcagt
     4261 ttgatgaagt gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc cgtaagccga
     4321 aagtaaaact ggtggagggt tatctggctg agcaagcgat ggatcgtgcc aacagttatg
     4381 taattggcga tcgcgcgacc gacattcaac tggcggaaaa catgggcatt aatggtttac
     4441 gctacgaccg cgaaaccctg aactggccaa tgattggcga gcaactcact aaacgagacc
     4501 gttacgccca tgtagtgcgc aacaccaaag agacgcagat tgacgtccag gtgtggctgg
     4561 atcgcgaagg tggcagcaag attaacaccg gcgttggctt ctttgatcac atgctggatc
     4621 agatcgccac ccacggcggt ttccgcatgg aaatcaatgt caaaggcgac ctctatatcg
     4681 acgatcacca caccgtcgaa gataccggcc tggcgctggg cgaagcgtta aaaattgccc
     4741 tcggcgacaa acgcggtatt tgccgctttg gttttgtgct gccgatggac gaatgccttg
     4801 cccgctgcgc actggatatc tctggtcgcc cgcacctgga atataaagcc gagtttacct
     4861 accagcgtgt gggcgatctc agcactgaaa tgatcgaaca cttcttccgt tcgctctctt
     4921 acaccatggg cgtgacacta cacctgaaaa ccaaaggtaa aaacgatcat caccgtgtag
     4981 aaagcctgtt caaagccttt ggtcgtaccc tgcgccaggc catccgcgtc gaaggcgaca
     5041 ccctgccctc gtcgaaagga gtgctgtaat gaacgtggtg atccttgata ctggctgcgc
     5101 caacctgaac tctgtgaagt ctgccattgc gcgccacggt tatgaaccca aagtcagccg
     5161 tgacccggac attgtgttgc tggccgataa actgttttta cccggcgtcg gcaccgcaca
     5221 agcagcgatg gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca
     5281 accggtgctg ggcatctgct tagggatgca actcctggga cgacgcagcg aagagagcaa
     5341 tggcgtcgac ttgctgggca tcatcgacga agacgtgccg aaaatgaccg actttggtct
     5401 gccactgcca catatgggct ggaaccgcgt ttatccgcag gcaggcaacc gcctgtttca
     5461 ggggattgaa gacggcgcgt acttttactt tgttcacagc tacgcaatgc cagtcaatcc
     5521 gtggaccatc gcccagtgta attacggcga accgttcacc gcggcggtac aaaaagataa
     5581 cttctacggc gtgcagttcc acccggagcg ttctggtgcc gctggcgcta agttgctgaa
     5641 aaacttcctg gagatgtgat gattattccg gcattagatt taatcgacgg caccgtggtg
     5701 cgtctccatc agggcgatta cggcaaacaa cgcgattacg gtaacgaccc gctgccgcgc
     5761 ttacaggatt acgctgcgca aggtgccgaa gtgttgcacc tggtggatct gaccggggca
     5821 aaagatccgg ctaagcgtca aatcccgctg attaaaaccc tggtcgcggg cgttaacgtc
     5881 ccggtgcagg ttggcggcgg tgttcgtagt gaagaagacg tggcggcgtt actggaagct
     5941 ggcgttgcgc gcgtggtggt cggttccacc gcagtgaaat caccggacgt agtgaaaggc
     6001 tggtttgaac gtttcggtgc cgatgcgctg gtgctggcac tggatgtccg tattgacgag
     6061 caaggcaaca agcaagtggc agtcagcggc tggcaagaga actcgggcat ttcactggaa
     6121 caactggtgg aaacctatct gcccgtcggc ctgaaacatg tgttgtgtac cgatatctcg
     6181 cgcgacggca cgctggcagg atctaacgtc tctttatatg aagaagtgtg tgccagatat
     6241 ccgcaagtgg cgtttcagtc ctccggcggc attggcgaca ttaacgatgt tgctgcccta
     6301 cgtggcactg gcgtacgcgg cgtaatagtt ggtcgtgcat tactggaagg taaattcacc
     6361 gtgaaggagg ccatcgcatg ctggcaaaac gcataatccc gtgtctcgac gttcgtgatg
     6421 gtcaggtggt gaaaggcgta cagtttcgca accatgaaat cattggcgat atcgtgccac
     6481 tggcaaaacg ctacgccgaa gaaggtgcag atgaactggt gttctacgat atcaccgctt
     6541 ccagcgatgg ccgtgtggta gataaaagct gggtatctcg cgtggcggaa gtgatcgaca
     6601 ttccgttttg tgtggcgggt gggattaagt ctctggaaga tgccgcgaaa attctttcct
     6661 ttggcgcgga taaaatttcc atcaactccc ctgcgctggc agacccaaca ttaattactc
     6721 gcctggccga tcgctttggc gtgcagtgta ttgtggttgg tattgatacc tggtacgacg
     6781 ccgaaaccgg taaatatcat gtaaatcaat ataccggcga tgaaagccgc acccgcgtca
     6841 ctcaatggga aacgctcgac tgggtagagg aggtgcaaaa acgcggcgcc ggagaaatcg
     6901 tcctcaacat gatgaatcag gacggcgtgt gtaacggtta cgacctcaag cagctgaaaa
     6961 aagtgcgtga agtttgccac gtcccgctga ttgcctccgg tggcgcgggc accatggaac
     7021 acttcctgga agccttccgc gatgccgacg ttgacggtgc gctggcagct tccgtattcc
     7081 acaaacaaat aatcaatatt ggcgaattaa aagcgtacct ggcaacacag ggcgtggaga
     7141 tcaggatatg ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa ccgacggtct
     7201 gatgccggtg attgtgcaac acgcggtatc tggcgaagtg ttaatgctgg gctacatgaa
     7261 cccggaagcc ttagacaaaa ccatcgaaag cggtaaagtc accttcttct cgcgcactaa
     7321 acagcgactg tggaccaaag gcgaaacctc gggcaatttt ctcaatgtgg taagtattgc
     7381 cccggactgc gacaacgaca cattactggt gctggcgaat cccatcgggc caacctgcca
     7441 caaaggcacc agtagctgct tcggcaacac cgctcaccag tggctgttcc tgtatcaact
     7501 ggaacaactg ctcgccgaac gtaaatatgc cgacccggaa acctcctaca ccgccaaact
     7561 gtatgccagc ggcactaaac gcattgcgca gaaagtgggc gaagaaggcg tggaaaccgc
     7621 actggcagca acggtacatg atcgctttga gctgaccaac gaggcatctg acttgatgta
     7681 tcacctgctg gtgctgttgc aggatcagga tctggattta acgacagtaa ttgagaacct
     7741 gcataaacgg catcagtgag ttacgtgctg agcaggtgtg atgttgttgt cacacccgga
     7801 gccattgccc gatgcgcaaa cttatcgtgc ctacacatta tctcaacaca cgtaggccgg
     7861 ataagatgcg ccagcatcgc atccggcaaa aaaaacgggc atggtgtcac caccctgccc
     7921 gtttctctta aatgcacaat aatattactt cgcgttgtaa ttacgcagag cgttacgccc
     7981 caacacaatc cccgcgccaa ccatgccgcc cagaagcact gccagaatca aggtgattgc
     8041 ctttttcgga ctatcgcgac gaataggtaa cgtcggtttc atcacatagc ggtaagcatg
     8101 aatatcaaga tcatcaaact ttaatttttc aatatccaac aggttttgac gtgtctgata
     8161 gtagtttggt gagaacacca acggacgagt cgcttcatgc ttaatcatcg actccagcgc
     8221 ttcgctccct agaaggaaca acgtatcttg cgtcacatct tcagtctgct gaacctgtgg
     8281 ctttgtcacc tgcgcctgat tcgcatactg caacgcttcc tgaatctgac ggatacgcag
     8341 atctttctgc tcctgcgcga ccacttcctg ggttcttaaa gagtcctgca agtttttccg
     8401 tcccagagca atgttgtcct tgagatcctt ttctagctct tgattcactt tatcatcaac
     8461 ttgctgaatg tattgggcca acttcatttg tgcgccctct gcagtttgcc caacataaga
     8521 aacagtcaat ggtaattgtt ggttcttaac agaaggttcg atggtaagtt tttctggctc
     8581 ttcctgatta tccagcgttt ctgctaatgc agagaaggca gaactgaagc gaccaattaa
     8641 cgtctcctgc aaatccgata ctttcggtgc agcctgacca tagataacat tcatggcatt
     8701 gttatagcca gcaatttgcc ccacgtcggg ctgagtgata attgctgttg acgtccattt
     8761 ctccttcgct actgccaaat aaccaatagc cagggcaata gccacaatga cggaaatgat
     8821 aattgtcatc ttgccacgcc acaactgcac tagtaaatca atcaaatcaa tctgttccgg
     8881 gtcatggttt tgcccagaaa cattattatt ttctactctc atcattaccc taactgacga
     8941 aaatttccaa gtccgcatag tttagcgata atccccgtga atgctatagg ataagtgata
     9001 aaaataatga attattgcgg aggtaacaaa agacaatcag ggcgtaaata gccctgataa
     9061 caagatgtta gtcgctgcca aagagatcgc gggtgtagac cttatctgcc acatccttaa
     9121 gctcttctgc catacggttg gagataatga cgtcggcttg ttgtttgaag gtggcgagat
     9181 cacgttccag gcgagagttg aagaatgagt cttctttcat caccggctcg tagatgatca
     9241 cttcaacgcc tttcgccttg atacgcttca taatcccctg aatagaagac gtacggaagt
     9301 tatctgaccc gctcttcata atcagacgat aaatacccac cacttgcggt ttgcgtgaca
     9361 aaatggcatc ggcaataaaa tctttacgcg tgcggtttgc atcgacaatt gccgagatca
     9421 ggttattcgg cacagactgg tagttcgcca gtaactgctt ggtatctttc ggcagacaat
     9481 aaccaccata accaaacgac ggattgttgt aatggttgcc aatacgcgga tcgagacaaa
     9541 cgccttcgat aatctggcga gtattcagac ctaaactttc tgcataacta tccagttcat
     9601 taaagtatgc tacgcgcatc gccagatagg tattagcgaa aagtttaatc gcttctgctt
     9661 cagtggagtc ggtaaacagg gtcgggatat tttgcttaat cgcgccttcc tgtaacaacg
     9721 cagcaaaacg ttctgcgcgt tctgaacgct caccgatgac aatacgtgac ggatggagat
     9781 tatcgtaaag ggctttacct tcgcggagga attccggaga gaaaataata ttttcagtac
     9841 gatatttctt atgcatcgct gcggtaaaac caacgggaac cgttgatttg atgaccataa
     9901 ccgcataagg atttatctca actacgtctt taattactga ttctacactg gatgtattga
     9961 aataattagt tttaggatca tagtcggttg gagtggcgat gatgacataa tcagcatccc
    10021 ggtaggcttc atttttatct aatgtggcat taaagtgtat tttatctgat tgcaaaaact
    10081 gctgaatttc cttatcaaca ataggagata tccgatcatt cagcatagca acgcgtgacg
    10141 gtaaaatatc taatgccaca acctcatgat tttgtgcgat tagaagcccg tttgacaagc
    10201 ctacatagct agtaccggaa atggtgattt tcatttcatg ctctcagaat taacttaact
    10261 gtgaatcatg atgtttttag catcgtgata agatgctaaa agttttaacg ctacgttgtc
    10321 agatggtcaa cgcaacgaaa aacaattcag attttcttta taagaatagc tgatgtttat
    10381 taagttaata ttaagcagtg aaatttagtc tataagatat ttggcaaaaa aaagcccggt
    10441 gcaatatacg ccgggcctca attttattgt tggttaaatc agattaatcc agccattcag
    10501 tatggaacac accttcttta tcaatgcgct tataagtatg cgccccgaaa tagtcacgct
    10561 gtgcctggat caggttcgca ggcagaaccg cggcacggta gctatcgtaa taggcaaccg
    10621 cagcggcgaa ggtcggaacc gggataccgt tctgtactgc ataagcgacg acatcgcgca
    10681 gcgcctgctg gtagtcatcg gcaatttgct tgaagtacgg ggccagcagc aggttagcga
    10741 tctgcggatt ttcggcataa gcatcggtga ttttctgcag gaactgcgca cggatgatgc
    10801 agccagcacg gaaaatcttc gcgatttcgc cgtagttcag atcccagttg tattcttcag
    10861 acgcagcacg cagctgagag aagccctgag cgtaagaaac gattttgccc agatacagcg
    10921 cactgcgaac tttttcgatg aactcagcct tgtcgcctgc tgactgagct tgcggaccag
    10981 agagaacttt agatgcggca acgcgctgat ctttcagaga agagatataa cgtgcaaaca
    11041 cagactcggt aatcagcgac agcggttcgc cgagatccag cgcgctctgg ctggtccatt
    11101 taccggtgcc tttgtttgct gcttcatcca gaatcacatc aaccaggtag ttaccgtctt
    11161 catctttttt ggtgaagata tctttagtga tgtcgatcag gtagctgcta agttcaccgt
    11221 tattccactc ggtgaaagtc tgggccagtt cttcattaga tagattcagg ccacctttga
    11281 gcagagaata cgcttcagca atgagctgca tatctccgta ttcaatacca ttgtgaacca
    11341 tcttcacata atggcctgcg ccgtcggcac caatataagt aacgcatggc tcgccgtctt
    11401 ctgccacggc agcgatttta gtcaggatag gcgcaaccag ctcataagcc tctttctgcc
    11461 cgccaggcat aatagaagga cctttcagcg cgccctcttc accgccggaa acaccggtac
    11521 cgatgaagtt aaagccctct gctgaaagct cacgattacg acgaatggtg tcctggaaga
    11581 aggtattacc accatcaatg atgatgtcac ctttatcgag gtatggtttc agggaatcaa
    11641 tcgcagcatc cgtgcctgca cctgctttca ccattaacag gatgcgacga ggcgtttcca
    11701 gagattcaac aaactctttc accgtatagt aaggagccag tttcttgcct ggattttcag
    11761 caatcacttc ttccgtcttt tcgcgggagc ggttgaaaat agagacggta taaccacgac
    11821 tttcgatgtt gagcgcaagg ttgcgcccca tcactgccat accgactacg ccgatctgtt
    11881 gctttgacat tgtttactcc tgtcttctat attattaacc gtctattaac cgttatatta
    11941 ttaaccgtcg tgattttcat cttctaatca aggaatacat atggttacag catgcttctt
    12001 actttatagc gataagtatg ccattgggtg tatccataag aactttgaat tttggatact
    12061 tttctggatt aaaattagca tagcttcgtt tagatataac aataaacgaa ctattaaaag
    12121 atttcatctt ctctattaaa tcatcctgtg ggattacctc acactcggga ttactaatag
    12181 tccaatcact atatcttgct aatccaaaga cgtcaccaat tgccatccct ttatagaagt
    12241 aaataccatc ctccaagcct aattggataa gattatctcc atataactca ctatattcac
    12301 ttgctttctt catcagttca tagctatagc gactttctaa gcgtgaatta aaattcaagt
    12361 attcataata aaaatatttg aattcaaaat gcaacgatcg ccccgaaaaa aaataataca
    12421 cacaaggata aaatatcgtc ccaacttgta atttgtaatg ttgatatctt ttattaaact
    12481 tgcaattgag atcgcaacca gcaatactga caatgggtaa atgggtgata aatacctgtc
    12541 accttgcgta acagaaaacc aaaataagac atatgttaaa acaatcgaga aaaataccca
    12601 aagtatttta ttattcctaa gtgtaaagac agacagtata gcataaaaaa caacatcact
    12661 gccaacttta ttaattgctt gttgcaaatc taatgtggca ggacttatac catgtcgagc
    12721 ttgttctgct gtttgaactt gtagatcaat ttcattccat aggtagtgac caaatatatt
    12781 tcctccagca ggactaaaag gatcaccaga gatccaaaaa ctacggacat accaataaat
    12841 gccaccaaga attgttgcca agctaaaaat aagaaatgtc tgtatgtttc tctttttcgc
    12901 tatcaatata acaaatatga tggctccata tatggcacca aatagtttta cgccacaagc
    12961 aattccaata aaaaaaccac ataaaaaaat taattcattt tgtgttttat tgttttctga
    13021 gcgaatatat ataagaatac tggctgcagc aaaacaaaga tagcgttaaa tcaatatatg
    13081 cataaccaag atgatcttta agtggattga tagtaaatag taggattgta gcaataatgc
    13141 taggataata agttttcgta atttcccatg aacagccaac aagtccacag gctattacaa
    13201 aaaaacagat atttgctaaa aactgagcta accttacatc tcctaactgt aaacctaaag
    13261 ccattaaaag atctgcattt tgcgggaaca gaggaaaacg caaatattgt tcaacaacaa
    13321 ttgcatggtg ctcaactaat gaacgagcca aaggtaagtg atacatagta tcatcccaat
    13381 caccgggagc tcgcagggac gataacaatg taaccacaaa aatcaaaaca agaaataaaa
    13441 taacaaacag cgtactgaac tcaatattat ttattgccag ccatctaaat tgctgacgtt
    13501 taataatgct taaaacccaa cacaccgtaa aaattaaaaa gatagcaatc ttattaaatt
    13561 gcccgaccat gcccagaaag taaattatca aaatatttaa agacatccct atgcataggc
    13621 aataaaaaaa taaagaaaat ttgttaagtc ctgccacccc ctccttaaaa aaagatgaca
    13681 atacaatatt accaatgaca aaaaatgtca ttccaaaagc aaaacaatac acaacataca
    13741 aatacaacgg caattcaatc atagtaaaat actctattta cattatcagt ttatagtttt
    13801 tttcagaaag ataaaataat gccttcattt cacgtctagt tgttgtattt gaatgcaaaa
    13861 taatgccaca tacaaatgcc attattgacg ataacatgat ggaggcagtt aaaaaggcag
    13921 tcggaaatct tctcaccaaa cctgtatcaa tataatcaac aatgatgggc aatcctaaaa
    13981 ttatagacaa agcagcaagt aaaacgccga taatactaaa aaagtatagt ggccgttcaa
    14041 gcgagtaaag tttaattatt gttttcagaa tcttgaaccc atcactccaa gttgatagtt
    14101 tgctcacact cccttcagat cgctcgccat attttgtatt aacctcacca tacttcatac
    14161 gtaattctaa agcatgaatt gtaagctcag tttcgatttc aaatcccctt gatagacatg
    14221 gaaaagattt aacatagcgt ctggaaaata cacgataccc cgacaacata tcagtaaaat
    14281 tccctttaaa gatagtctga acagtaccag tcaacagttt atttcctcag cgatgccctt
    14341 ttctgtatgt attttgatct ccattctctt gacgacatcc tacaaccata tccaattgat
    14401 tttttatgag atggttaatt atttctggag cagaacttgc atcataagtg ccatctccat
    14461 caaccattag ataaatatcc gcatctacat ctgagaacat acgtcttacg acattcccct
    14521 tgcctttcag aggaacagag tgaactgttg ctccagcatc ctctgccact tgtgctgtac
    14581 tgtcagtcga attattatca aatacatata tagaagcagt tggaatattc gttttgaaat
    14641 catttatgac ttttgctatt gccttctgct cattataaca aggaataatt acagcgatat
    14701 taaaagagtg atacataagc ccctcatata aactatttag ctaatgtgca agtaattgtg
    14761 tttttaacaa atcgtttact aacgaaaaat gaaatatttt gatgcgcaat atcctaaaac
    14821 caaactaaaa agagaaaagg tgaacaatgt aaatattgga gataaagcta agagatcaaa
    14881 taaaacgccg aataaataac tcatcgcccc cataaaaaat ataaatataa aatatctagc
    14941 cttggttgca gatactttaa atgagcatct tgcatttgca aaaaaagaaa aggttgctgc
    15001 acatataaaa ccaacaatgt tacttcggcc ttgaccaaaa gctaaattat tataacacgc
    15061 ataaaatacc ccccaatgta aagcagtatt gacaattcca attaaaaaat aactaaaaaa
    15121 agaactggtc aataacttcc ctatttttaa catcctttat tttgctccag aagtgaggtt
    15181 attactaatt tggatatttt ctatagaaaa tacccataaa actacaacgc ctataataaa
    15241 aattcccatg ggtgtaaaaa gaaatggatt agttatagaa acggtaatac tgaaaaaaag
    15301 tattaacgac atcattaatg atgtttttgt actatttaaa aaatagcctt tcaaaaagat
    15361 aagcaacata ggaactaatg atataaatac accaataacg cctgttttct gaagtatctc
    15421 aagaggtact atttcaatcc ttaatcgatc taatataaat gaaccaaatc ctcttccaaa
    15481 caagaacgtc gctaaatcaa catttttata ataaaaatat aaatcattaa atctaacaga
    15541 gtcagaatct tcactgcgtg atccgaccat gtaaagcata attataaata gaacgctgcc
    15601 caatattatt ataatagtat ttaaatatag cttatgtaat ttaaaactgg ctaataacag
    15661 tgataatgtt gtaaaaagta atacacctct tgattctgtc aataatatcg caagcactat
    15721 tatgagacat tttaaatatt ttttgtcgac aaaactgata atcgcaccga caccaaaaaa
    15781 gtagaaggat ttggaaaaaa aagccccatc gggacgaaaa aagaactcgc tagtcaatga
    15841 taagtgttca taaattaaat taaaatttaa caaaccgaaa ttcaacaaaa ttatgtatga
    15901 aatatataac agtgatccat ataatgcaaa tgcattaact attttaataa aaatctcaca
    15961 taaaaatact tttaaagtat atcttagtgt aaatagatat ataagtgaca gcataaaaag
    16021 gtatggttgc acatcaaaca gtatatcatt tattttatta ccagctaata agccgacaaa
    16081 gaagccataa aaaggaaata aaagcaacca tataaaaaga ctgacacatt tttgggtgat
    16141 tgaaaaattg aatgtaacta aattaaaaat taaaagtata aaactaaata caaataataa
    16201 atatcgcaaa gggaatattc ccaatggctc cagtaatctt ccaccaccac caataatcag
    16261 ttcaatacat aaaaatgtta taaaaatttt atttatatta ttcatattcg taaggtgatg
    16321 ttttttaaaa tatgtttaaa atcaacatta ataaaatata aattttatca gcctttttcc
    16381 gccttaactt ccaaaaactt aaagccaaga tcaaattgcc tatataacct ttacccaatt
    16441 gattattcac aaagctatca tcagctaagg cattagctat tttagttact tcttttcgat
    16501 accacttgtt tctcaccaaa ccaagccgtt tgtattttgc tttaaaacta atgtttgctc
    16561 ccacttggtt gttttcatgc tgtctgtaca gcattgttgg ttttctatca atataccacg
    16621 aataatttct cgtccgcgca aatgaatata aaaaccaatc atgcagacag atatcttgtg
    16681 cagcattttt attatttatg atgaacttct taaattcaat aagcgtttct ttttttataa
    16741 catatgtaca ccccggacct gctgcttcaa aaaaataatc gaattgagtt tgcgactgag
    16801 ccttatcaac taaatttttt tgccctgatg gataatatgc aattacattg ctggaataac
    16861 aattcgcatt attaaatacc aagcagtcaa ttgcattttt tattttatct ttataccaaa
    16921 tatcgtcctg atcactaaaa cagatataat catagttttc aagatcgaca tctttaatta
    16981 atctataaaa atttttacct gcccccccaa acttattgac gcttggaagt atatttataa
    17041 ttgagggata tgataattga aattcttcac aaatatttaa tgtattatca gttgataaat
    17101 catctgatat aaaaattgag atgtctacat ctttttgttc aataatactt tttaattgtt
    17161 cctcaatcca gcattcgcca ttatacgtag ccataagtac agctactctt ttttgatcag
    17221 aactatgcat tataacgacc gcccccagta attcctctta ttccatccag cgtagccaaa
    17281 atatgttttt tcatatattc aattttattt ttttgagttg cgataattaa agtcgtatga
    17341 attaaagaaa aaacaacttc cctaatagag agtaaaagag gcacatgatt ttttctaagc
    17401 aaaagaaatg catttcttac tcgataataa cgacgtactg gcgagtgaat tggtatgtta
    17461 atcccacaga tttttactga cttatcccca atagcatgaa ccatttctat atcaggaatg
    17521 atatgaacta atatgccata gtttaagcat ctaagacacc attctgtatc gacataatca
    17581 ataaaaagtt cctcatccat cattccaaca atctcaaaaa cttcttttga aaccatggtt
    17641 ccggatgaga ttgttactga acttttaaac ggtgtctgtc cttcagtgac atgaattttc
    17701 tctcgaagac catttttttt gatattacaa ataggataat aatgtgactt atctctatca
    17761 ataaaaactg ggccagctat ttttgcattt tcattatcca tatagcagga aagttttttg
    17821 ataaactcat tgcgaatacg gctatcttga tcaaaaaaaa ttatcttctc tgcccctttt
    17881 tcaagagaat atcgaatccc aatattttgt gcttttgcta taccttcatt cctgccaaga
    17941 caaattaaat gaactttttc aatgtctgct aatttatctt tcaaggtgaa attagtccta
    18001 ttatcgacta ctacaacgta acaattttgc tcctttagtt cagtgatcag tgcattcaga
    18061 tttttaagtt cgggattata tgtcacaatg acagcgtaaa tattactatt cattatccaa
    18121 gtgactcagt aattggttaa ttttagtttt catatacaga acacattcta gtataaagcc
    18181 attgtaataa cagagcatct aaaattacac gtatgctcca ggatattgcg gcacccagta
    18241 acccatattt ttcgaccata aaatacaaaa gggctaaata aggaaccaat tcagcacaat
    18301 gaatcaaagc agtaatttta gattttccta atgcctggat tgcagagaat ggtatcattg
    18361 ccagcgcatt gaagaaaaaa ccgatcaata acacatttaa tatattaaag gaattaattt
    18421 ggtaattctc accaaaccat attttcaata caagccctga atataacaag cctatgatta
    18481 tcaccggcaa acagattaga aacattaaaa gcaatgattt atttacatta cgtttaaatt
    18541 ctttaaagtc tttgatgtta cttaaccttg gaaacactgc cctcccgatt gctgcaggta
    18601 ttattcctaa ttttagaata acctccgctg gtgcagaata aaatgcaact ttatctgccc
    18661 ccataatatt tgacactata aatcgatcaa aatacaccat gaccggactt atgatattac
    18721 ttactgtcat ccaaccgcca aagaaaaaga gtcgtttaaa ggttttataa caaaaatgaa
    18781 cccccgaaat tttaatatca ttcctgacat aataggcaga gactaaaatc gaaataactc
    18841 ttgcaaatat taaaccagca accgctgccg acaatgtagc actataaaaa acaaatatgg
    18901 ccggaattcc cgcgatgcaa gagcttgata tagatttttg aatatttaca atgccaaatt
    18961 tttcatcccc ctcaagaatg gctgaccata attgatttag aataaataat ggaatgcaaa
    19021 tagccaacag tttaaatgct agttgtatat cactatgttc aacaccagaa atttttagat
    19081 aattaacaat tccatcagag aaaatcaata ataaaaaagc accaaagcat gaaaatagca
    19141 ccaaaaaaga tgtacttgtt gaaattacct tggttctttc atgatgatta tcacgatgaa
    19201 ttgcaatttc tctaataaca gagcgagtca ggcctacatc aaaaattcca gcatacccaa
    19261 caagtgcaat tgcaattgta taaacaccga aaccttcagg ccccaaacct cgagcgagaa
    19321 atcctagcga ggggatagca attaaagttg gtattgcata gccaaaaagg ttccagacac
    19381 tattttttat tatgctcatt attacatttc tttatcatat aaaataatca gcatctacaa
    19441 atttttgacc attaatatct ttatcagaaa gcaatggatt ttgaaccggc cattctatat
    19501 ttatactttt gtcgctccat aaaatcccac gctcttgcat ttttgaatag aaattatttg
    19561 ttttgtagac aaattctgct tcatcactaa gaacgcaaaa cccatgagcg aatccttccg
    19621 gtatccataa ttgacgctta ttctccgccg acaaatttac accaacccac ttgccaaatg
    19681 ttggtgatga tttccgaata tcaacagcta cgtcaaaaac ttcaccgaca acacagcgta
    19741 ccagttttcc ttgagcataa ggttccagct gatagtgcag tccgcgtaac acacccttag
    19801 ttgatttaga atggttatcc tgaacaaact caacctttcg tcctacagcc tcttcgaaaa
    19861 ctttctgatt gaagctttcc ataaaaaagc cccgctcatc accaaatact tttggttcaa
    19921 aaattagcac atcaggaatt tcagttttaa ttacgttcat tttattaata acctttaatc
    19981 attttcagca gatactgacc ataagcattt ttcttcagcg gctcggctaa taccttcact
    20041 tgctcagcat caataaagcc tttacggtaa gcaatttctt ccgggcagga aactttcagc
    20101 ccctgacgct cttcaattgt tgcaatgaag ttgcttgctt caataagact ttgatgtgtc
    20161 cccgtgtcca gccatgcata accacgcccc atcatagcga cagacaaacg cccttggtcc
    20221 atataaatac ggttaatatc ggtaatttcc agttcgccac gtgcagatgg tttaaggttt
    20281 ttcgccattt ctacaacgtc attgtcatag aaataaagcc cagtaaccgc atagttactt
    20341 tttggttcca gcggtttttc ttccagacta attgccgtac cgttatcatc aaattccacc
    20401 acaccgtagc gctcaggatc atttacgtga taggcaaaaa ccgttgcgcc actctctctg
    20461 ttaacagcgg tatccattaa tttcggcagg tcgtgaccat agaagatatt atcaccaagt
    20521 accagcgcac aatcatcacc acctataaat tcttcaccaa tgataaatgc ttgcgccaga
    20581 ccatccggac tcggttgtac tttatattgc agattaagcc cccactggct tccatcacca
    20641 agcaattgtt gaaaacgtgg tgtatcctgc ggcgtactga taatcagaat atctcgaata
    20701 cccgccaaca taagcgttga aagcggataa taaatcatcg gtttatcata aatcggtagc
    20761 agttgtttgc tcacagccat tgtcacagga taaagacgag tgccggaacc accagccaga
    20821 ataatacctt tacgcgtttt catttcacta ttccttttaa ttcatctcgc tctggcatca
    20881 tgagcgagat gcaataattt gttaaattgc cgtggtcgta aataattcgt tgagcatacg
    20941 tttcacgccc acctgccagt caggcaagac aagcgcaaag ttctgctgaa atttttcggt
    21001 attgaggcga gaattatggg gacgacgtgc tggtgtggga taggccgttg tcggcacggc
    21061 attaagtttg ttaagagcaa ggttgatccc cgctctgcgc gcttcttcaa ataccagagc
    21121 ggcataatcg tgccaggttg ttgtaccacc agcaaccaga tggtacaaac cagcaacttc
    21181 tggtttattt gcggccacgc gaatggcatg agcggtgcaa tcagccagta attcagcccc
    21241 cgttggtgcg ccgaattgat cgtttattac agccaactct tcgcgttctt ttgccaggcg
    21301 caacatcgtt ttggcaaagt tatttccttt accagcgtat acccagctgg tacggaaaat
    21361 aagatgcttt gcgcaatgtt cttgtaacgc tttttcccca gctagcttgg tttcaccgta
    21421 gacatttagc ggtgcggttg catccgtttc cagccatggc gtgtcaccgt tgcctgggaa
    21481 aacataatca gtggagtagt gtataaccca ggctccgact tcattagctg cttttgcaat
    21541 cgcttcgacg catgtcgcgt taagtaattg cgcgaaattc ggttctgatt ccgctttgtc
    21601 tactgcagtg tgagcagcag cattaacaat aacatcgggg cgaatttttt tgacggtttc
    21661 agccacacct tcggggttgc tgaaatctcc acaataatca gtggagtgaa catcgagcgc
    21721 aatcaagtta cccagaggag ccagagcacg ctgcagttcc caacccacct gccctgtttt
    21781 gccgaaaagc aggatattca ttactgacgg ccctcatagt tttgttcaat ccacgactga
    21841 taagcaccgc ttttcacatt ctcaacccaa tttgtattag ccagatacca ttccaccgtt
    21901 ttacgaatcc cgctctcaaa cgtttcttgc ggtttccagc ccaattcgcg gctaatttta
    21961 tcggcatcaa tggcgtaacg gcggtcatgc cccgggcgat cggcaacata agtaatttgc
    22021 tcacgataag atttctcttt cggtacaatc tcatccaaca aatcacaaat agtgagcact
    22081 acgtcgatgt ttttcttttc gttatgtcca ccaatgttat aagtttcacc cgctttacct
    22141 tcggttacga cggtatataa cgcacgagca tgatcctcta catacaacca gtcgcgaatt
    22201 tgatcccctt taccataaat aggtaatgcc ttaccttcca gagcattcag aataaccaat
    22261 ggaataagtt tttccgggaa gtgataagga ccataattgt tcgagcaatt agtcacaatg
    22321 gttggtaaac cataagtccg cttccaggca cgtaccaaat gatcgctgga agctttagac
    22381 gcagaatacg ggctacttgg cgcgtaagct gtcgtttccg taaatagcgg taacgcttcg
    22441 ttattatttg cttcatccgg atggggtaag tcaccataaa cttcatcagt agaaatatga
    22501 tgaaagcgga aacttttttt cttttcatca ttcagagcag accagtaatt gcgcgccgct
    22561 tctaaaagaa cataagtccc cacaatattg gtttcaataa atgccgcagg gcccgttatt
    22621 gaacggtcaa cgtggctctc tgccgccagg tgcatcaccg cgtctggctg gtgctgtgcg
    22681 aaaatacgcg acatggctac agcatcgcag atatctgcat gctcaaaagc ataacgttca
    22741 gaatcagaaa catcagcgag tgattctagg ttcccggcgt atgttaattt atcgacatta
    22801 acaacactat cttgcgtatt atttattatg tgacgaacaa cagcagaacc aataaaccct
    22861 gcgccaccag taacaagtat cttcactttt ttattccata tagctataga gcatgctgtg
    22921 aaatagactg ctccagattt gattaataga ggtattaata cacgctaccg cccctggctt
    22981 aacagctacc agtgcactgc atgcatctca acgatgtggc gagcgttagc cactcgcgcc
    23041 aaatccgaaa aattcaaacg ttaattgtct taccaaacag ccgtagaaac aaggaaaatc
    23101 ctggaaaaat ttgaataata tgagctatgt aacttcttgt ttttataggc tttataaaaa
    23161 cggcaacggc aggaaaattc aatgagcaaa acttatgcca cgaatcttca ctgccgttat
    23221 aattttctta tcaaccgtta catccggtca gattttcatt attcgcttaa cagcttctca
    23281 atacctttac ggaacttcgc cccttctttc aggttgcgta ggccatactt cacaaacgcc
    23341 tgcatatagc ccattttttt gccgcagtcg taactgtcgc cggtcatcag cattgcatca
    23401 acggattgtt ttttcgccag ctcggcaata gcatcagtca gctgaatacg tccccatgca
    23461 ccaggctgag tacgttccag ttccggccaa atatcggcag aaagcacata gcgacctacg
    23521 gccatgatgt ctgagtccag cgtctgcggc tgatccggtt tttcgataaa ttcaacaata
    23581 cggctgactt taccctcacg gtccagcggc tctttagtct ggatgacgga gtattcagag
    23641 aggtcacccg gcatacgttt tgccagcacc tggctgcggc ccgtttcgtt gaaacgtgca
    23701 atcatggcag caaggttgta acgtagcggg tcggcgctgg catcgtcgat cacaacgtct
    23761 ggcagtacca cgacaaatgg gttgtcacca atggcaggtc gcgcacacaa aatggagtgg
    23821 cctaaaccta aaggttcgcc ctgacgcacg ttcataatgg tcacgcccgg cggacagatg
    23881 gactgtactt ccgccagcag ttgacgcttc acgcgctgct caaggagtga ctctaactca
    23941 taagaggtgt cgaagtggtt ttcgaccgcg ttcttggacg cgtgagttac caggaggatt
    24001 tctttgatcc ctgcagccac aatctcgtca acaatgtact gaatcattgg cttgtcgacg
    24061 attggtagca tctctttggg tatcgcctta gtggcaggca acatatgcat cccgagaccc
    24121 gctacaggaa taactgcttt taaattcgtc attatttcat ccacctgtaa aatggttgct
    24181 gaattatagc ttgttcgatt tttttcgcca gcatcaatta ccctgaattg attactgaat
    24241 tacttgtgat gttacgccgc ttcgttgtgg attgcagtag cattgttcct aagtatggct
    24301 ccatttttcc aggaatggtc gcaaatctac tccctccgtt ccggcaatct aaagttaatc
    24361 ttttccacat taacaatatg gtgattaatc ctgtcgatat cgacggagct ttgccctctt
    24421 tcattcaccg catgaacatt tgcaagagac agcagtgttt cttttttcgc cataaagacg
    24481 ccgcgaacgt ctttgcgcat gtcgaagttc atgctcaatg ctggtccaac tgaggattcc
    24541 tgcatcacat tgatatttcg tataaaaaga tgttgcggct tgttgtgtaa ctccagcgag
    24601 gcacgcttca tctcaatgtt agttagcgac acaaaggaga cggcattccc ggcggagatt
    24661 tggatgccgc gcaatttata agcaagatgg gtattatcca gttgaatatt attcactcgg
    24721 aaattttgcg gtatcgagag atatttgcct ttaattaccc catagccgat taacatcccg
    24781 gcgctattaa tcatttcaat attatcaatc acgaaattgt cacaaccgta aatagcgact
    24841 gtcgcgttat caatgcccgc tttcttactg aaatccggcg tgatattgcg ggcattgata
    24901 ttacgaataa caaaatgttt accattttca acatggttca actgccgaca atccgatccc
    24961 gtgatattcg ccacgacaaa gtttttcact gcctggtctt ccgggtagtt gttatcataa
    25021 gtgcttcccg caaggcctat gccgatgccc cagttgattt tgccgttggt acagttgata
    25081 cgctcgatga catggtcaga tatcaaaata tcactgtcgt taattgccac gttccattca
    25141 atggcgtcgc cctgtaagtc gctgaactta cagttggtaa tgttggcacc gataatctgg
    25201 ttatgaaatc cctggcgtaa gatggcgtaa ttagcgtggc tgacagtgag gttatcgatg
    25261 gtcaggttgc gcatgacccg tttgttttta ccgccaatat aaatctgcat taccgggcca
    25321 aagccgctca tcgccagccc tttgatggtg cagtcagaac cacgcacatc cagggtaatg
    25381 ttatgcatac cgccgccctc tccccctgtc acctggctgc cgtcctgtaa gacaaatcgc
    25441 cctctgccgt tgccgcgcag gcttccaagg atgtgtaacg ttttgccggg agggatgaag
    25501 atgccggtgt tgatattgtc acaaacgaat ccggcaggca cgacgaccgt ttgcccttcg
    25561 ctgaaggctt gtttaaatga ggcgatccag tcatgtgggt tgtagtcgtt aatgttaacg
    25621 ctttgtcggg cgggaagtgc gcgagcgaaa ggggtatgga ggaaggcaag cgccgagctt
    25681 gccgtcagga aggtgcgtcg ggagagtgtt ttaaatggca tacgttctcc tctataaagc
    25741 ctgcagcaag ctggcgagtt ctcgattaat cacctgctgg ttaaaatcgt gttcgacttt
    25801 ttcgcgcgca cgtttgacga ccggagccag ttcgtcggta tccagttggc taaacgccgc
    25861 cagacgttgt gccagtgcgc gagcatcgtt ctcaggcacc agccagccgg atttatcggc
    25921 ctccaccagt tcaggaattc cgctatgcag agtagaaacc accggaatac cgaccgccat
    25981 cgcttccatt agcgccaccg gaatgccttc catatcgcca tccgcgcccg ttaccgatgg
    26041 caacaggaag acatccgcgt cgtcgagcat ctccttcact tcatggctcg gtttaaagcc
    26101 cggcatctct accacatctt ccagttgata ttgttcgatg agggtgcgca ggcgtcgttc
    26161 ccacaggcca atgccgagga tgcgatagcg aaatgccacg ccctgctctt tcaactgacg
    26221 gcaggcttca atcgccacat gcaggccttt tttctcggtt aagcgtgcga cagatatgat
    26281 ttccagcggc gttgcgggcg ctttcaccgg acgcgggcta aagcgcgtca tgtccacgcc
    26341 catacgcgat acggcgattt tttctctggg gcagcccatt ttttgcagcc ttccggccca
    26401 cagatcgctt atcggtaaca tcaggtcgcc acggcgaaac agttgttgat attcgggagt
    26461 gtagtggttg agcacttccc gactggagat atcaataccg tggaagatgg tggcaatttt
    26521 gccgcgaatg atacccagtt cgcgtagttt tgctgcggtt acccccgccg ggccaaaatg
    26581 agcgatgaac acatcggcgc gaaacggtgt tgcgacctga ccacaaatgg cagacaaaat
    26641 caggttccgc gactcggcac catagcgttt gaggttgagc gcatgccagg tatttttacg
    26701 atgaatgcca cgtaaggtct ggctggcgcg gtggcgcagt ttcgctactt tgcccgcagg
    26761 ttcgtcctgt aaccagcggg ttctggcgac aaggttgtat ttcgtccatg ccgcgtgggt
    26821 gttctctgta tcgccttttt gcaaagcgac aatctccact tcaaagccca tatcaataaa
    26881 cgcggtaatt tgattgagga cgaaggtttc cgacgacagc gggaatttca ataagaagaa
    26941 gccgaccttc atttcacctc cccgatgcgc tcaagcacag actgcaccat ctgcattccc
    27001 gtctgacgtt cgcgactgac ggcttcgtta aatcgagcgt tcagcaccgg aagctggcct
    27061 aaggtatccg caaccatcgc ttgcaggctg ccgtccagta aatgacggat atcaatcgcc
    27121 atctctggca gtcccagctg ctgcataatc ccggcggatt tatgttcata gttgatggca
    27181 atcgccggag tagcaaagtt cattgagata atggcggagt gcaggcgcgt accgacggtg
    27241 agttcacagg ctccgagaat tttgcccatt tccagatcgt tgagttcatc catcactacg
    27301 tggtaacggg caggatcgct gatgtgctgg cgcagattga gcgccaccat gcggtcgtct
    27361 ttgttatagc tgtcgatacc cgtacaggtg gagagcgcaa tcacctgata gccttcatcg
    27421 agaatgcgat tgaccacccc ggcaaaggct ttttcatacg cttgttgagt ggtgccgaga
    27481 cgtttgtcga acggtgccag ttcgcgcagg gtaattgcca ctgttttctg ttgtgcggca
    27541 acgtccagcc agtgttgaac ggcatagctg gcggtgaagt cttcggtgtg gtgatcgacc
    27601 agccacgcgg tatcgacgcc atgttccact tttgcggtgg tgatattgct gcgtttcatc
    27661 aaatcaaggc tgaccgattc gcgcaggatc agcgcgtcgc agtgaccaaa gacatagttc
    27721 gccagttggt taaattgctc atcctggaag gggccgacgc tgtgaccaat cataaacagc
    27781 ggctttttcg ccataaatgt gcaaagcgca tgttcaaact gcggcacgcc gtagagatcg
    27841 acaaaaaacg atccgccgac ctgaataatg gcgtcgtaac ctgacagcag gcgcacgaag
    27901 tcggtgaatc cctgggcgat ggcgatattg cgcagcttgc cagtgtcggt gacgcgtgag
    27961 agcaatacct gatgctggta gcggcgacgg aggacttttt taacgcgccc gacaacgccc
    28021 gccgcgctat tgtgttgtgt catctgcagg aacagcggat cgcccattac cgggcggttg
    28081 agcagccagg aagaactgac cggatagcgg ctcatcacgt ccacttcggt atgtggattg
    28141 aggatgttga tggcatcaag taagccgcgc aagatggcgc tgtcgccacg attgccgcaa
    28201 gtgtggttgc ccagaataag taatttcatt ttttcctcat aaatttgatg ccaggtgagg
    28261 ctgtgtttgt tgtgttcgtt gccgtttttt gcctgatgcg acgctggcgc gtcttatcag
    28321 gcctacaggt cccgagcaca gaaccgtagg acggataagg cgttttacgc cgcatccggc
    28381 aaccgttgtc ggaaccgaaa acagcaattc accccgcccg taaaagcatt ttcatttttt
    28441 cgctgcgaca aaactgacgc ttcacttcca ccaccagcgg atggcgtgac agcacaatca
    28501 tcaccacaaa cgccagcacg cccgcggcta tttgcaccgc cagcagcatc cccagcgcca
    28561 gttgcccttt cagcacaatg cccagcgcat aactgaccac cagcgtcggc aacgagagat
    28621 aaaacggcag ccacaaactc aggatgtact ggcgataact ggaaccaaga accggtttaa
    28681 tcatcacgaa gtaactcaga atggtgttga taatctgcac cagcaggaaa ccgagcgtga
    28741 cgccgattgc gcccgccatc tgcccaccaa taacaatcgc cggaataaac agaaatgttt
    28801 tgaatacatt gaatttaaag ctgatatcga cccgcgcttt cgccatcagc agcgaaccaa
    28861 tcgggttacc cacggagcgc agcagaccca caatacacag caattgcagc accggaataa
    28921 tgctgttcca cttttcgccg aataccagcg gtacgaagtt attcgacacc accatcagcc
    28981 ccagcagcgc cggaaagttg ataatcccca ctatcgacag cagcttgtag aagttaacac
    29041 gcagcttttc ggtgtcgtcc tgaattttgg caaacgccgg aaacaacacg cgggtgatga
    29101 ttgggttcag cttcatcggc ggtacgacgg ccacgttgta cgccaggttg tatccccctg
    29161 ccacgcccgc gccaagaata cgcgccagca ccagcgttga aaggttggtg ttgagatagt
    29221 tgatgatgct gtccgccgtc agccaggcac caaaacgtaa gttcggtgcc acagacgcca
    29281 gcgagaagtg cagaccgggg caataaattt tgcgtccaaa gtagccaaac agcagtgttc
    29341 tcaccgcact attgaccaga taaccgagga tcgcggtcat cgccagcggc cagaaatggg
    29401 cgctaactac cgtaaaagtg aagcccgcca gcaccgcgct ggtttcgatc atgccgattt
    29461 tgttgaactc cagctccttc tgcatcaacg cgcggaactg ttgcccgtgg gggatcacca
    29521 caaacgccag cgacaatgtt ttaatcaacg gtgccaggtc cgggttattc agcacgtcgc
    29581 cgatgacatc actcaacaaa aacaccgcca cacataccac aagccccagc ccgacgttca
    29641 gccagtacag cgtggtgagt tcaaggtgac tgatttcttt tcgctgaata atcgagttag
    29701 cgataccgaa gtcagaaagc gtatctgcca gcgcgataat caccagcgac acagtcagca
    29761 ggccgaactg gtggttgtcg ataatccgcg ccagcacggt catctgcacc agcccgaggc
    29821 cgatgatgat caccgtggca atagccgacc acttcgcgcc gctgatggtt ttttcacgta
    29881 agctcatatc aatatgccgc tttgttaacg aagcccttga ataccgtcag gaaaacgatt
    29941 ttgatatcga accagacgct ccattcgcgg atgtactcaa ggtcgaactc gacgcgtttt
    30001 tccattttct ccagcgtgtc ggtttcgccg cgccagccat taatctgcgc ccagccggta
    30061 atgcccggtt tcactttatg gcgcagcatg tagccttcaa tgagctggcg atactgttcg
    30121 ttatgcgcta ctgcgtgcgg acgtggaccg acaatcgaca tccccccggt cagcacattg
    30181 ataaactgcg gcaattcgtc cagcgaggtg cggcgcagaa agttccccac tttagtgacg
    30241 cgcggatcgt tctgcgtcgc ctgggtcacc actttgtcgt tctccatcac tttcatggaa
    30301 cggaacttcc acactttgat cggcttgcca tccataccat agcgagtctg gcggaaaata
    30361 accggccctg gtgaactgag tttcaccgcc agcgcaatac agcacagcac cggggagatc
    30421 agcagcagaa taagcgtcgc cagcacaatg tcttccgcac gttttagcag gcggttaccc
    30481 cccgaaagcg gcgtgtcata cagcggcacc accggaacgc cgttcatctc ttcgagacgt
    30541 gaatggagaa tgttgaaggt aaagacgtcg gggatcagca gcaccgaaca ggtggtatcc
    30601 gccagttgat gggccagttt tttcactcgt gcgccgtcgc acatttgcat cgcgatatag
    30661 acgttatgaa tcttgcccgc ttttgcatcc tcgaccagct gttgcaggtt acccgcccag
    30721 tcgttagaaa cgccgcccgg tttcgggtcg tggtaaacgc ccaccacttc aaaccctaac
    30781 cacggctgat tacggaaact ctccatcagc atttgtccgg cggctaaatc ccccgctacg
    30841 gcgaccatcc gcttgttgta gccatgatta cgcagccagc ccgccccaat gcgaatacat
    30901 gaacggcaaa ccaccagtcc gatgctggtc agcccatacc acgccagcca gattttcagt
    30961 tgcgtgtcga aatcattgtt gaacgccacc agtccggcgc tgaaaatcac gcttaaggtc
    31021 cagttttgta gcaacagggc aaattctgtc gctgcccgaa caccgcgcca tgagcgataa
    31081 aaatcggtga tgccgcccag catctggaac accaccagcg taatcagcgc caccaacagg
    31141 tgcatgtaga ggaatgacag tccgctgact tcgcaaacca gccatagccc ggcaaacatg
    31201 atggtgatat ctgaaaagcg ttgcaccata gagattaacg atgcattggt tttcgctcgc
    31261 tcgcgctttt ttagatttgt catcgttgtt cctgttatta gccccttacc cgcagtgggg
    31321 taagggaaga tccgacatta ctcgttcagc aacgtcagca gagttcgcgt tcgcgcttcc
    31381 atcagcggca catcaccgcg cgattccaca ttcaggcgca ccaccggttc ggtattggag
    31441 gtgcgcaggt taaagcgcca gtcggcaaag gtcatgctga tgccatcgct gcgatccacc
    31501 gccagcgcct cacggctaaa atgctgttcc acgcggttaa tcgcctcaac gggttgcgcc
    31561 agtttgctgt tgatctcacc gcttgccgga aacgccgcca tccggtcgcg caccagttcg
    31621 cccagcgttt ttcctttcag gcacaccagt tcggcgacca gcagccacgg gatcatgccg
    31681 ctgtcgcagt aagcgaaatc acggaagtaa tggtgggcgc tcatttcgcc accgtagatg
    31741 gcgtcttcct tgcgcatccg ttctttaata aaggcgtgtc cggttttcga cattaccggc
    31801 gtgccgcctg cagcagtcac cacatcaata gtgttccagg agagacgcgg atcatggatg
    31861 atcttcgcgc cgggattttt ttcgaggaac gcttctgcca gcaggccgac aatgtagtag
    31921 ccctcgataa actgcccttt ttcgtcaaac agaaaacagc ggtcaaaatc gccatcaaag
    31981 gcaatgccca tatccgcgcc gtgtttgatg accgcattgc gggtgtcgtc gcgacattcc
    32041 ggcagcagcg ggttaggaat accgttgggg aaattgccgt ccggtgtgtt gtgtactttg
    32101 attaattcca cgggcgcgcc gagggcttta aagcgggctt caatggcgtc caccaccgga
    32161 cccgctgcgc cattcccgga gttgatcacc agcttgagcg gcgtgagatt cttgacattg
    32221 atataaccga acaggtgatc aacgtaagcg tcacgcaggt tgatttgctg atagcgaccg
    32281 cgtttggttt catcgacggg aggaaagtcg ttggcttcag ccagacgctg gacgtcgcgc
    32341 agtccggtat caccgctgat cgggcgagcc ccctcgcgca ccagcttcat gccgttgtaa
    32401 tccatcggat tatggctggc agtaacttca atgccgccat ccacgccgag atggaacgtg
    32461 gcgaaataga tctcttcggt gccggacata ccgatatcca gcacatcgac gcccgcatcc
    32521 tgtaaacctt ttgccagcgc cagttttaag gtttcgctgg tgagacggac atcaccgcct
    32581 aacacaatgg ttttcggttt gagaaattcg ccataggcgc gaccaatgcg ccaggcgata
    32641 tcttcattca gttcttcgcc taatttcccg cgaatatcat aggctttaaa gcaggttaat
    32701 tttttcatgt cgttaccctt tttcaggcaa tagttggccc tgaccgaaac ggccaatttt
    32761 atttttgtcg ttatttacac cgcggtttcg cattcattgc ctgatgcgac gtttacaccc
    32821 gtccgtagcg atccgcgaag cgcaccacat catcctcttc gagataagag ccggagcgca
    32881 cttcaattaa atcgagcgga attttccccg ggttttccag gcagtgcgtc gcccccagcg
    32941 gaatataaat ggactcgttt tcaccaagca gtttgatatc accatcaatg gtgacttttg
    33001 ccgttcccgc gaccactacc cagtgttccg cgcggtgatg gtgcatctgt accgacaaac
    33061 cctcgcccgg tttcacggtg atgcgtttca cctggtagcg gtcgcccgcg tcgatagagt
    33121 catatttgcc ccacggacga tacacttcgc gatgcacccg atgctcatgg cgaccatcgg
    33181 ctttgatctg ctcgaccact tttttcacat cctgtaccgc gttacggtcg gcaatcagca
    33241 ccgcatcttt ggtctgcacc actaccagat ctttcacccc gacggtggtg actaggcctg
    33301 attcggcata cacatagctg ttttcagttt tgtgattaat cacatcgccg tggcaaacgt
    33361 tgccctcggc ggtgtgggcg ctgatctccc ataatgaaga ccaggaacca acatcgctcc
    33421 agcccgcatc catcggcacc acaacagcat ctgccgtacg ttccatgacc gcgtaatcca
    33481 ccgactcttc cgggcaggcg agaaacgctt cttcatctac acgaataaaa tcgagatccg
    33541 gatcgacggc gctcatcgct ttttcgcagg catcgaggat atccgggcga tattttttca
    33601 gttcttcgag atagcgtccg gcgcggaaca ggaacatgcc gctgttccag taatattcgc
    33661 cgcttgctac ataggcctgg gcggtttcca gattcggttt ttcgacaaac tgcgccactt
    33721 caaaggccac cgtatcctgc tcacccgcag acacttcacc gcgacgaata tagccataac
    33781 cggtttctgg tagatccggc acaatgccaa aggtcaccag cttgcccgct tcggcataag
    33841 gcatggcatt acgcacggcg gcacggaacg cgtctttatc ggcaatcaca tgatccgccg
    33901 ccaataccag cattaacggg tcgctctccg ggctatgacg ttttgccgcc agcgccgcca
    33961 gtgcaatggc aggtgcagtg ttacgccctg ccggttcgag aataatgttc tcggtgagtt
    34021 tgttcagttg acgcagctgt tccgcgacaa taaagcggtg ctgctcattg caaatcacca
    34081 ccgggctttc gcactccacg ccgttcaggc ggcagatggt ggtttgcagc atggtgagat
    34141 cgcctttcag gcataaaaac tgcttggggt aaagtacgcg ggaaagcggc cataaacggc
    34201 taccggagcc acctgccatc acaactggat agagtttcga ctgcgccata attatccccg
    34261 aatatcattt ataaattgac gtaacacgtt ctctttatcg agcgtgcgtt cggcatattc
    34321 acgtgccacc gtgttatgtt tgggcagcag gagcgcctga cgaatccccg ccaccagcgc
    34381 ctcgaccgat tccggttcta cgcaaaccgc aatacccgga aaggtttcgc aaagctgccc
    34441 cagttctgtg tgggcctctg cggtaatcac cgcgttaccg cctaccgcca gaatattggt
    34501 cagtttcgac ggcaatacgg catctgccgc gccgcgtttt tacaccacca gatggcaatc
    34561 gcccatcttc agcagtgcgg gtaaagcgtc atacgattgc agcgggaaaa attgcatgtt
    34621 gcgcagtcca cgctgctgcg ccattttttc cagccgcgct ttgccgccgc cctgcccgac
    34681 aatggcaaaa agcagcggtt catcgcgcag gagatcggca gcttcaataa cgttttccag
    34741 cccctgcttt tcaccaatat tgccggagta aagaatgatt tttttgttat ccggcaggcc
    34801 aagctggtta cgaagggcat caacatcggc gtctgcaaca tgctgaaaac gggcaatttc
    34861 cgaccagttg gggaagaaga tgacgttatc cgccgccacg cctttttcga tggctttatt
    34921 catcatcgaa cgcgaaatcg tggaaacgtt atcgacgtta tgcagtccgc tacgttcgaa
    34981 cgccgttgcc agctgtgcca ctttgccgcc tttgcctttt ccggcaaggc ccagccccag
    35041 catggcgtcc acttcgtaat cctgaatatg cagcacggta cgcgcaccag agagcttcgc
    35101 cagcaggcgc attcccggcg tgcaaaacag cgttggcact acgccaataa tgcgatccgg
    35161 cttccagcga cgttgcgcca tcagtggaaa gaaactgctg acggcaaaac tgccgagatg
    35221 caacaagcgt ttcagggtgc tcggctgttt tggcacgtac agccaccgtg gcggcccctt
    35281 cttctcgttt gtagcgccag gcggaatagt tctcacccac ctgccattgc gggtagtaag
    35341 gcggtgcggt aatgacccgc acctcatgac cttgtgccgc caaccattcc accatctcgc
    35401 cggtgtattt gccgatgccg gttaactccg gcgagtagtt aatgccgtag accagtattt
    35461 tcataatccg ggtactccgg cacgcttctc agcgagaaaa taggcgcggc tgttagcgtg
    35521 aacatcgtta ctggcgagca gcgcgtccgg cgtcagccag cggtaatcgt catgctgctc
    35581 atccggcagt aacagctctt cttccgctac tctgaagcga aaaccgagca ccacatagtg
    35641 agtggtgaaa tccgtgccgg agaagttatc gtcataaaaa tgctgccaga caccgtaaaa
    35701 ctggcctgct gttatcggca gacgcagccc cagttccgcc atcgtcagcc gctcaaatgc
    35761 ggcttccagc gtttcgtctt tctgcacgcg ccctcccggc acaaaccagt aaccctgcgc
    35821 cgggcggttg gttcttttgc caagcagaaa ctcgccgcga ctgttctcga caataaagtc
    35881 gagagagaca agcggtgtgg agcgcactac agtggcaaag tcttcctgac gtaaaaacat
    35941 cattcccccc gaaagcggtc ttgattctca aggaaccact ggtaagtgct ggcaagcccc
    36001 gcttccagtg agatttcgtg ataccagcca agctgatgca ggcgcgtcac atccagcagt
    36061 ttgcgcggcg taccatccgg tttgctggca tcaaaaacca cccgaccttt gtaacccacc
    36121 actttggcga tggtttgcgc cagctcgcgg atagtgcagt caacgcccgt gccgacgtta
    36181 atgtgcgaca gcatcggttg gctattctcc agccagactt catgcgccag ctccatgaca
    36241 tgaatgctcg ccgccgccat atcatcgacg tgcaggaatt cacgcatcgg tgtaccgctg
    36301 ccccatacca ccacgtccgg cgcattctgt gccgtcgcct cgtggaagcg acgcagcaat
    36361 gctggtatca catgggagtt actcgggtgg aagttgtcat gcggcccgta caggttggtc
    36421 ggcatgacag agcggtaatc gcgtccatac tggcggttgt aagattcgca cagtttgatc
    36481 ccggcgattt tggcaatggc gtaaggctcg ttggtcggct ccagcgtgcc ctgcaataac
    36541 tcgctttctg ccatcggctg ttttgccagt ttcgggtaga tgcaggacga tccgagaaac
    36601 atcagtttgt tcacgtcgtt ctgatgcgcg gcgtgaatga tgttgctctc aatcatcatg
    36661 ttctggtaga tgaaatccgc cggataggtg ttgttggcaa caatgccgcc cactttcgcc
    36721 gccgccagat agacctggtc aatgcgttcg ctggcaaaga aatcatgcac ggcgcggctg
    36781 tccagcaggt tcagttcatc gcgggtacgt aataccagct ccacatcacc gcgctgttcg
    36841 agctgccgct tgatggcaga accgaccatc ccacaatggc cagcgataaa aatgcgttgt
    36901 ttacttatgc ttatgactcc agcgcgatcg ccacgtcgta gccgtgagat ttcagcagag
    36961 agtgtttttt cgccgcttcg aggtcattag ccaccatttc agacaccatc tctctgaggg
    37021 tgatttccgg tttccagccc agtttttcgt gcgctttggt cgggtcgccg agcagcgttt
    37081 caacttctgc cggacggaag taacgcggat caacggcgat aatcacatca cccggtttaa
    37141 cgcccggcgc gtcatgcccg gtgacggaaa ccacaatgcc cttctcttca acacccgtgc
    37201 cttcaaagcg cagtttgatg cccagctgtg ctgccgccat ttccacaaac tgacgtacgg
    37261 agtactgaac gccggtcgcg ataacgaaat cttccggctg ttcctgttgc agcatcatcc
    37321 actgcatttt tacgtagtct ttggcgtggc cccagtcacg cagggaatcc atatttccga
    37381 ggtacaggca cgactccagc ccctgggcga tgttggcgat tgcgcgggtg attttgcggg
    37441 taacgaaggt ttcgccgcgg cgcggggatt catggttgaa gagaattccg ttacaagcgt
    37501 acatgccgta ggattcgcgg tagttaacgg tgatccagta ggcgtacagt ttggcgaccg
    37561 catacggaga tcgcgggtag aacggcgtgg tttctttctg cggaatttcc tgcaccagac
    37621 catacagttc agaggtggaa gcctgataga aacgagtttt cttttccaga ccgaggaagc
    37681 ggatcgcctc cagcaggcgc agcgtaccca tcgcgtcgac gtcagcggta tattctggtg
    37741 actcaaaaga gaccgcaacg tggctcattg cgcccaggtt gtacacttca tccggctgta
    37801 cttcacgcaa aatgcgcgtc aggttagagg tatcactcag gtcgccataa tgcagatgga
    37861 atttcgggtt gcaggtgtgc ggatcctgat aaatgtgatc cacgcgctcg gtgttgaatg
    37921 acgatgcgcg acgcttaata ccatgcacct cgtaaccttt ttccagcaga aactctgcca
    37981 ggtaagaacc gtcttgtccg gttacaccgg tgatgagagc gacttttgac atgtcttatt
    38041 cctctgtatt tttgaattta ttcagtttca acgcgttcgc gtatcaccac tgcgggattc
    38101 ccccggcaaa ccacatttgc cggaagcgat ttaaaaacac tgcttcgtgc acccacgacg
    38161 gtgccgtcgc cgattgtgac gcctggggca acaaagacat cggttgccag ccagcatttc
    38221 tcgccaatca caataggcgt ggcgttaatg gtgaaatgtt gacttgcatg gtcgtggcta
    38281 ccggtgcata aataactttt ttgcgatatc accgaatgtg cgccaatggt tatttcaccg
    38341 agggtatata aattgacgtc atcgccaacc cacgcgtaat cacctaaggt taatttccac
    38401 ggataggtaa tttttactga cggacgaata actacgtttt ttcctatttt tgctccgaat
    38461 aaacgtaata aaaaagcccg ccagcgatac aatacttgtg gcgaccaggc aaatattgtt
    38521 gcctgtactg cccaccataa ttgcacttta atagcgttgc cgccccggaa ccctttcggc
    38581 accgagaaac cgcttaaatc ttgcattggt tttccttata ttcagacttt gttatataag
    38641 gctttagtct tacctgtcgt acgttggcgt aaatgccagg ataattctgc ccagaagcca
    38701 ggtacatgta atatctgccg ttggactttt ttggcgtcag cac
//
LOCUS       DADMEQ010000023        38559 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.023, whole genome shotgun sequence.
ACCESSION   DADMEQ010000023 DADMEQ010000000
VERSION     DADMEQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.023"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            12..88
                     /locus_tag="JNZ05_001261"
     tRNA            12..88
                     /locus_tag="JNZ05_001261"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46..48,aa:Met,seq:cat)
     gene            complement(163..1416)
                     /locus_tag="JNZ05_001262"
     CDS             complement(163..1416)
                     /locus_tag="JNZ05_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HAY8564561.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1648..2979
                     /gene="argA"
                     /locus_tag="JNZ05_001263"
     CDS             1648..2979
                     /gene="argA"
                     /locus_tag="JNZ05_001263"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HAY8564562.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3041..4867)
                     /gene="recD"
                     /locus_tag="JNZ05_001264"
     CDS             complement(3041..4867)
                     /gene="recD"
                     /locus_tag="JNZ05_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HAY8564563.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMLL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4867..8409)
                     /gene="recB"
                     /locus_tag="JNZ05_001265"
     CDS             complement(4867..8409)
                     /gene="recB"
                     /locus_tag="JNZ05_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HAY8564564.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEVIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNDDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8402..11290)
                     /gene="ptrA"
                     /locus_tag="JNZ05_001266"
     CDS             complement(8402..11290)
                     /gene="ptrA"
                     /locus_tag="JNZ05_001266"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HAY8564565.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKTEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11466..14834)
                     /gene="recC"
                     /locus_tag="JNZ05_001267"
     CDS             complement(11466..14834)
                     /gene="recC"
                     /locus_tag="JNZ05_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HAY8564566.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14847..15170)
                     /locus_tag="JNZ05_001268"
     CDS             complement(14847..15170)
                     /locus_tag="JNZ05_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAY8564567.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15155..15562)
                     /locus_tag="JNZ05_001269"
     CDS             complement(15155..15562)
                     /locus_tag="JNZ05_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HAY8564568.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(15559..16122)
                     /locus_tag="JNZ05_001270"
     CDS             complement(15559..16122)
                     /locus_tag="JNZ05_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY8564569.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16113..16583)
                     /locus_tag="JNZ05_001271"
                     /pseudo
     CDS             complement(16113..16583)
                     /locus_tag="JNZ05_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(16767..17561)
                     /gene="thyA"
                     /locus_tag="JNZ05_001272"
     CDS             complement(16767..17561)
                     /gene="thyA"
                     /locus_tag="JNZ05_001272"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAY8564570.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17568..18443)
                     /gene="lgt"
                     /locus_tag="JNZ05_001273"
     CDS             complement(17568..18443)
                     /gene="lgt"
                     /locus_tag="JNZ05_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAY8564571.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18594..20840)
                     /gene="ptsP"
                     /locus_tag="JNZ05_001274"
     CDS             complement(18594..20840)
                     /gene="ptsP"
                     /locus_tag="JNZ05_001274"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY8564572.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSI
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKEMAAIFDLYSHLLSDTRLRRELFAEVDKGLVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20853..21383)
                     /gene="rppH"
                     /locus_tag="JNZ05_001275"
     CDS             complement(20853..21383)
                     /gene="rppH"
                     /locus_tag="JNZ05_001275"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HAY8564573.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            22068..22757
                     /gene="mutH"
                     /locus_tag="JNZ05_001276"
     CDS             22068..22757
                     /gene="mutH"
                     /locus_tag="JNZ05_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HAY8564574.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22826..23539
                     /locus_tag="JNZ05_001277"
     CDS             22826..23539
                     /locus_tag="JNZ05_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY8564575.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23677..23895
                     /gene="ygdR"
                     /locus_tag="JNZ05_001278"
     CDS             23677..23895
                     /gene="ygdR"
                     /locus_tag="JNZ05_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HAY8564576.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            24003..25043
                     /locus_tag="JNZ05_001279"
     CDS             24003..25043
                     /locus_tag="JNZ05_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HAY8564577.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25076..26269)
                     /gene="lplT"
                     /locus_tag="JNZ05_001280"
     CDS             complement(25076..26269)
                     /gene="lplT"
                     /locus_tag="JNZ05_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HAY8564578.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FCPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACVLAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26262..28421)
                     /gene="aas"
                     /locus_tag="JNZ05_001281"
     CDS             complement(26262..28421)
                     /gene="aas"
                     /locus_tag="JNZ05_001281"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HAY8564579.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSVITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28810..29841
                     /gene="galR"
                     /locus_tag="JNZ05_001282"
     CDS             28810..29841
                     /gene="galR"
                     /locus_tag="JNZ05_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HAY8564580.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDENGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29848..31110)
                     /gene="lysA"
                     /locus_tag="JNZ05_001283"
     CDS             complement(29848..31110)
                     /gene="lysA"
                     /locus_tag="JNZ05_001283"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAY8564581.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31232..32167
                     /gene="lysR"
                     /locus_tag="JNZ05_001284"
     CDS             31232..32167
                     /gene="lysR"
                     /locus_tag="JNZ05_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HAY8564582.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32154..32846)
                     /gene="ygeA"
                     /locus_tag="JNZ05_001285"
     CDS             complement(32154..32846)
                     /gene="ygeA"
                     /locus_tag="JNZ05_001285"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAY8564583.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGKFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(32975..34388)
                     /locus_tag="JNZ05_001286"
                     /pseudo
     CDS             complement(32975..34388)
                     /locus_tag="JNZ05_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
     gene            complement(34703..35464)
                     /gene="kduD"
                     /locus_tag="JNZ05_001287"
     CDS             complement(34703..35464)
                     /gene="kduD"
                     /locus_tag="JNZ05_001287"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HAY8564584.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(35494..36330)
                     /gene="kduI"
                     /locus_tag="JNZ05_001288"
     CDS             complement(35494..36330)
                     /gene="kduI"
                     /locus_tag="JNZ05_001288"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAY8564585.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVAVKD
                     LR"
     gene            complement(36650..37798)
                     /locus_tag="JNZ05_001289"
     CDS             complement(36650..37798)
                     /locus_tag="JNZ05_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAY8564586.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKAQALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGG"
     gene            38053..38211
                     /locus_tag="JNZ05_001290"
     CDS             38053..38211
                     /locus_tag="JNZ05_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter"
                     /protein_id="HAY8564587.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTY"
BASE COUNT         9508 a        10517 c         9431 g         9103 t
ORIGIN      
        1 cagtacggtg acgcggggtg gagcagcctg gtagctcgtc gggctcataa cccgaaggtc
       61 gtcggttcaa atccggcccc cgcaaccaat tattgaacac cctaacgggt gtttttttgt
      121 ttctgatctc ccataaaaaa agcgccattc agcgcctttt tatcatcccc ttctcgccag
      181 cgttgcccca tcggcaaaat acgctttaat ccccgcaaga atagactccg caacttcctg
      241 ctggaaagtc gccgttttca gtttacgctc ttcctcaacg ttactgataa acgccgtttc
      301 gaccagaatg gagggaatat ctggtgcctt tagtacggca aacccggcct gttcaacttg
      361 atttttatgc agcttgttga tttttccgag cttattcagt accgctttac caaacttaag
      421 gctgtcagca atagtcagcg actgcaccat atcgaacatg gtgtggtcga catagcggtc
      481 accgcttttg ctcacgccac caatcaagtc cgaggcgttc tgggtttgtg ccagatattt
      541 tgccgcagta ctggttgcgc ctttggttga gagcgcaaac accgaggaac cgctcggctg
      601 acgactggta aaggcgtcgg catggataga gacaaacagg tcagcacgct gtttctgggc
      661 ttttgctacg cgcacttgca acggaatgaa gatgtcttca ttgcgcgtca tgtacacctt
      721 catattgccc tctttctcga tcagagagcg caaacggcga gctatttgca ataccacatc
      781 tttttcgcgc gttttgtatt tccccaccgc accggaatct tcgccaccgt ggccagggtc
      841 aagcataatg acaatcggac gatcgcgccc tgctttaccc ggttgtggac cactttgcgc
      901 tggcggcacc tgcttttcga ggtcgccttt gttgtaatcc tccagcagcg ccagcagcgg
      961 gtcctgcata tcctgtgcat tggccggata gaggtccatc accagacgct ctttaaaccc
     1021 ggcgaccggc gcaagggcaa acagctgcgg ttttacgttt tgctttaatt caaaaaccat
     1081 gcgtacggtt tgcgggtcaa attgcccgac gcgcgccgac ttgatgaacg ggtcgtctgc
     1141 acgaatttgt gccgccatcc ccttgagtac cgagttcagg tttacatctt cgatatccac
     1201 caccacgcgt tcagggttac tcaacgcgaa ctgcttatat ttcagctgac gatttgattc
     1261 taccgtcacg cgggtgtagc tggacgcagg ccagacgcgc accgccacga cctggctaac
     1321 cgcagccagg ctgacctgac ttacgctcaa tagccacata gcgcccgcgc cttgcagtaa
     1381 acgacgacgg ctgattgcag tgttggatcc tgacatgcct ctcccgagca aaagaaatct
     1441 aagctgtgta acaagtaaac gactaatttg accggaaact ttagcgaaag acgcataatc
     1501 tgtcatctaa taaacggtaa acattctttt tatattcacg gcattactga taaaaaagtc
     1561 gctctcgcat aaaatttaca cttgcaccct gcgaaaaaac agaataaaaa tacaataatt
     1621 tcaaataatc atgcaaaggg gtgtgccgtg gtaaaggaac gtaaaaccga gctggtcgag
     1681 ggattccgcc attcggttcc ctatatcaat acccaccggg gaaaaacgtt tgtcatcatg
     1741 ctcggcggtg aagccattga gcatgagaat ttctccagta tcgttaatga tatcgggttg
     1801 ttgcacagcc tcggcatccg tctggtggtg gtctatggcg cacgtccgca gatcgacgca
     1861 aatctggctg cgcatcacca cgaaccgctg tatcacaaga atatacgtgt gaccgacgcc
     1921 aaaacactag aactggtgaa gcaggctgcg ggaacattgc aactggatat tactgctcgc
     1981 ctgtcaatga gtctcaataa cacgccgctg cagggcgcgc atatcaacgt cgttagtggc
     2041 aattttatta ttgcccagcc gctgggcgtc gatgacggcg tggattactg ccatagcggg
     2101 cgtatccggc ggattgatga agacgcgatc catcgtcaac tggaaagcgg tgcaatagtg
     2161 ctaatggggc cggtcgctgt ttcagtcact ggcgagagct ttaatctgac ctcggaagag
     2221 attgccactc aactagccat caaactgaaa gctgaaaaga tgattggttt ttgctcttcc
     2281 cagggcgtta ctaatgacga cggtgatatt gtctccgaac ttttccctaa cgaagcgcaa
     2341 gcacgggtag aagcccagga agagaaaggc gattacaact ccggtacggt gcgctttttg
     2401 cgtggcgcag tgaaagcctg ccgcagcggc gtgcgccgct gtcatttaat cagttatcag
     2461 gaagatggcg cgctgttgca agagttgttc tcacgcgacg gtatcggtac gcagattgtg
     2521 atggaaagcg ccgagcagat tcgtcgcgca acaatcaacg atattggcgg tattctggag
     2581 ttgattcgcc cgctggagca gcaaggtatt ctggtgcgcc gttctcgcga gcagctggag
     2641 atggaaatcg acaaattcac cattattcag cgcgataaca cgactattgc ctgcgccgcg
     2701 ctctatccgt tcccggaaga gaagattggg gaaatggcct gtgtggcagt tcacccggat
     2761 taccgcagtt catcacgggg cgaggttctg ctggaacgca ttgccgctca ggcgaagcag
     2821 agcggcttaa gcaaattgtt tgtgctgacc acgcgcagta ttcactggtt ccaggaacgt
     2881 ggatttaccc cggtggatat tgatttactg cccgagagca aaaagcagtt gtacaactac
     2941 cagcgtaaat ccaaagtgtt gatggcggat ttagggtaag cgatgaaaat caccggatgc
     3001 ggcaggcgta acactcgtgc gtcgcatccg gcaattacgt ttattcccgc gaactaaaca
     3061 acgccgccag accactgcgc cgctcagtac gagtggcgat tgccgcactt aatatacgct
     3121 catcggcata cagcgacaga cgacgacgcg cgcgggtcac cgcggtgtat accagctctc
     3181 gcgttactac cggcgtgcgt tggctcggta aaatcaacgc cgcatggtcg aactccgatc
     3241 cctgcgattt atgtaccgtc atcgcccacg tagtttcgtg ctctggcaag cgactcggtt
     3301 gcacagactt aatattgccg tccggcatcg caaaccagac gcgcgtcccc tgcccgcgat
     3361 ccagcgcaat accgatatcg ccattaaaca acccaagcgc gctgtcatta cgggcaatca
     3421 tcaccggtcg gccttcgtac caacgagagt gcggatgacg atgaattttg cgcttctgtt
     3481 gcataaactg ctcaattcgc tcattcagtc cagccacgcc aaacggccct tcccgcaggg
     3541 cgcacaaaag ctggtactca ttgaacgcct gaatgattaa atccggctcg gcacgcgctt
     3601 gcagcagatc cagataacgt ccgtaacccg caagagcttc ctcaagcatc gcaatataat
     3661 cttcgccgct ctgtaaaagt cgtttttcga tatcagtaaa atcctgctga aaaacggttt
     3721 tcactgccgt tttatcacca cggttgatcg ccgcagctaa ctgaccaatg ccagaatcgc
     3781 tgccgaaacg atagcttttt tgcagcaggc agagactgtc gcgcaaagat gccgcttctg
     3841 tgccggttcc tgccggaacg tgagtccccg tcaggcggct tagctgcctg gcacgctcgg
     3901 cggtaaagcc cgcgttggca taagcgcaga tatcgcccag cacagcccca gcctcaaccg
     3961 aggccagttg atcacgatcg ccgagaaaga tcactcgcgc atgatctggc aaggcgtcga
     4021 tcagtctcga catcataggc agatcgatca ttgacgcttc atctaccacc agcacatcaa
     4081 gatgcagcgg gttaccggca tgatgacgta aacgctggct acccggctgc gcgcccagca
     4141 atcggtgcaa agtgctggca tcttccggaa tgcgtttctt ttgttcatcg gtcagcggta
     4201 actgtcgcaa agccttgccg agagattcgg ttaagcgcgc ggcagcttta cccgttggtg
     4261 cagccagacg gatacggcag cgttcgccgt cggccatttg aattaacgct gccagcaact
     4321 tcgccacggt ggtcgtttta ccggtgcctg ggccgccgga aatcaccgag atccgccgcg
     4381 tcagcgccac tgccgctgca actttttgcc agttaatttc atcgcttact ggaaaaagtt
     4441 tgtccagggt ttgcgccagt agagcttcat caacctcaat ggtatgattc acctcattga
     4501 agaagcgcgc taccgtccgc tcgttacacc acatgcgatt caaataaaga cgatcgccac
     4561 agaggagcat cggcgtgggt tcatctcccc tgctgaccgc ttgagaagcc agcaagcatt
     4621 cttcccaatt ttgtagctca ccgatttcac tgacacaggt cgccaacagc ggatgcgacg
     4681 cctcgttatt ttccagtcgt gaaagcggca aacagacgtg tccctctccg gcatcatgac
     4741 ttaacagtgc cgccgcgagg gtgacagcag gatgttcatc tcccgccacg gtcagggcaa
     4801 attgcacatc cagcgggcgt agctgtttgt gctccacagc ttccagtaat tgcttttgca
     4861 atttcattac gcctcctcca gggtcatacc ggcaaacatc tcatccatca gggcaatcaa
     4921 cccggcgttg ggtcgggttg tgtaaatccc ttgttgcgga tgttctttat caacgccacg
     4981 caggaacaga tagataacgc cgccaaagtg aagatcatag tcgtaatcag caatgcgatg
     5041 gcgcagataa cgatgcagtg ccagggtata aagctgatat tgcagatcat agcggtgtgc
     5101 ctgcatcgct gctgccatag cctgttgggt gtaagccgaa ctgtcttcac ccagccagtt
     5161 ggatttatag tcgagcaggt aataacgccc ttcgtggcgg aataccaggt cgataaagcc
     5221 ttttaacatg ccgcgtacct gcatgaactc cagcggcggg cagccagcgg atagcgggtc
     5281 aaactggcga attagtgcat caagctggct ggcgatgagc ggttcgctaa tcggcaggta
     5341 aaactccatc tccacctgtt tatcgcggtc ggaaagctga ctcaggctaa cacccgtttc
     5401 attgagaggt gcctggagga cagccgtgat ccactcggtc aacaccggtt cccactgcga
     5461 ttcaaagccg ccgagttcca gtttttcctg tacccagttc gggtcaaccg gctgggtaaa
     5521 atcgaggtct tcaaacaaac tgtgcaagaa tgtccccggt gacgcaccgc gcgggaactg
     5581 atgcggtgtt aacgtcggtt cttcaacgac gctgaccacg cctgcggcat caacatccag
     5641 ccgtggcatc agatcctgag cgataccgtg accacgctgc tgcaagccgg agtagctggt
     5701 gacacgccag ttatcgccgg gcaatcgttg tagtgtcctc gcattcagtt ctgcagttaa
     5761 agcatcatta acctgccagg gctggttatc accagtttgt gccgtttgcc aggcaatatc
     5821 atcatcgcat aacgcttcaa tacaggtgcg aagccctgcc gcatcttgcg gttccccttt
     5881 ttgcagcaaa cgcccgagcg cactttggtg gacgtcggtg tcaccttttt tatcgccacg
     5941 acggcgcacc agcggtgcaa cgccgagact gcaatgccaa accgaacgcg tcagcgccac
     6001 gtaaagcaaa cgcagatctt ccgccagacg ttcgacctcc gcgaggtcga cgctttctgg
     6061 cgcagcatta agatccaaaa ctgcctcaaa cgagtggcga tcgtgataaa acgcctgatc
     6121 ctggacgcgg aaattggtaa taaatggcaa ccagaccagc ggatattcca gccctttcga
     6181 tttatggatc gtgacaatct gcaccagatg tttatcactt tcgaggcgca tttgttggct
     6241 ggaggcatta ctgtctggct cgaggatatg ttgcgataac cagcgtacca gcgcatgttc
     6301 actttccagc tgcgttccgg cttcttgaag cagttcgctg atatgcaaga tatcggtaag
     6361 acgccgctca ccgcctgccg ttgccagcaa gttttctgca atgttacgcg ccgacatcag
     6421 cgcccgcagc atcggcataa cgccacgttt gcgccagatt tgccgataac catcgaactc
     6481 ttcgactacc gcatcccacg catgttcgtc attattcagc gtttcaatat ccagcgcatt
     6541 cagccccatc attgacgttg ccagcgcact gcgcagggtg ttctcacgtt cgggcgtcat
     6601 caccgcctgc aacaaccaaa gcatttcctg cgcttccaga gtttcaaaaa cactgtcgcg
     6661 gttcgaaagg taaacggaag ggatttccag caacgttaag gcatcgcgca cctgggcggc
     6721 ctcctggcgg ctgcgcacca gcacgctgat gtccgaagca cgcaccggac gcgcgtcgtc
     6781 gtcgttcatc agcaacgctt cgccccgctg tccggcttgt agccagtcgc ggatttgcgc
     6841 agcacatacc tgcgccatgg tactttgata gtcgccaacg ccgcagcttt cgccttccat
     6901 cagccacatt ttcatcgcag gctgcgtttc acctttaaat acaaaacgta acgcctgatt
     6961 tttcccggct gatttcactg gaataaacgg tatttcgcga aacatgaacg cgtcatcagt
     7021 ctggctgaaa agcttgttca cactgttcac cattccaggt gcggagcgcc agttggtgtc
     7081 taaagtgtag tgggcgtgaa cttcactacg cgccttcata taagtgaaga tatccgcacc
     7141 ccggaatgca tatatggcct gcttcgggtc gccaatcagc aacaatgcgg tttccggctg
     7201 atggtgccag atacggcgaa aaattcggta ctgctggggg tcggtatcct gaaattcatc
     7261 gatcattgcc accgggaatc gcgtacggat cgccgctgcc aacacctcgc cgctttcgct
     7321 acgcagcgcg gaatcgagcc gacttaacat gtcatcaaaa cccaattcgc cacggcggcg
     7381 tttttcacgc gctactgttt cgcggatctc agccaatgct cgggtgatca ccagatcgcg
     7441 gatcgacaat ggttctgcaa gcagttgatc gatcacctca aacagtggat gtcgcggggt
     7501 ttcccccccg gccttcgtgc gatcttctaa gaaacgctgg gagaattttt ccagcgactc
     7561 cggcaactga taactgtttg tctcttcttc tgcccaagcg ctgatctttt cgatccattt
     7621 agcctgattg ctacggttaa acttgcgtcg atcaatacca gaagattcga tcagcgcatc
     7681 cagttcaccc actgcgtcgc gccactgctg ttttaccgta tcaatacgcg ccacaatttg
     7741 cgcgtggcgg gaagccagcg tttcatcatc gggcggcggt gctttgataa ccggcgcttc
     7801 gccttgcaga taacgattaa tatcgcgcag caacgcctgc ggccctttcc aggtttcaaa
     7861 gacgacctgg gctatttcac gcggcagcgg gtagcagtgg cgacgccaga aatcggcgca
     7921 ggcctggtag cgtagcagag actcatcttc aatcagctgc tgctcaaaca gcatgccgga
     7981 ttcaaaggca ttcaggttga gcatgcgctg gcaaaagccg tgaatagtaa agactgccgc
     8041 ttcatccatc tgccgttcgg ctaacaacaa ccactgcgcg gcttgcgctt tatcgtcgat
     8101 ctcttccagc aggcgttcgt acagtggatt gtcggtggtt tcacgcagac aggcgatgcg
     8161 caactcgtgg atattgctac ggatacgacc gcgcaattct gccgtggcag cctcggtaaa
     8221 ggtgaccacc agcagttctt caacgatcag cgggcgggga aaggcggcgg aaccgcctag
     8281 tccaagtaac aggcgcaaat agagcgccgc aatcgtaaag gttttgcctg tgccggcaga
     8341 ggcttcaatc aggcgctcgc cctgtaaggg caagcgcaaa ggatctagtg tctcggcgac
     8401 atcactcatt cttttcactc atcaggggca ttgtttgctg caacgcgctg acgttctccc
     8461 acactttcca gccttcagga tgcacatatt cggctttccc gttctggctg ccggaaatct
     8521 gcgacagaat agccatgcct tgcggctcga ccaccgcctg atggaagaaa tcagcaagtt
     8581 tttgcggcgt cagcagtttt atctgggcca cgattttatc acgcgaatcg aagcgcatat
     8641 tgccgcgatc gaaatcttta cttaacttcg atgcttcttc gccgagcgtt tgcggtgcct
     8701 gcagcatctg ggtaattacc gcctgctgga tttgcgcaaa ctcatctggc ttcatcgctc
     8761 gcaattttgc ctctgcggtt gggaaaaacg ccttgtaacg ctcccacaag aatgaaggct
     8821 gtttatcatt gctttgcaaa aggaagccca tgccccactg acgccccacg ctcattggaa
     8881 acgcaaacac ggcatagccc aattgttctt cggtacgcaa ctgattgtag aaccacggct
     8941 gtacgatctg ccccaacaga gagctatagg ctgagctggt gtattcatcg tagccagtcg
     9001 gtacaaatac cgctgccagt gcggagtcgg tgctgttacc ggctttttca aagatgacgg
     9061 attgtttttt atcgaccact acatctttgt ttcgacacca ctctgaacca tcagcgccca
     9121 actgtttttg cacatcgcgt gccagcgttg ttgcctgggc ctcggtcatg ttgccgataa
     9181 ccataaactc tggtcgagcc cctgatttta aggcgtcgcg ataggccagc acctctttca
     9241 acgtaatgga gggcaaaatt ttacgccgtt catctcgcga gaagtacggc acttgcgaga
     9301 gcatctgcgc gggcataatc gcctgctcaa acgctttacc cttttctgcg gaatccatca
     9361 tctggttata ccaggacttc gcctgctcaa gctgctcttc cgtagcggta tagctaaagt
     9421 acccctcgag caatgcctgg aacagctgcg gcagacgctg ggtgtaacca ttagcattaa
     9481 ccataaggcc gttgttagcg ttggtggaaa aacttatgcc accaaccgac gcctggttgc
     9541 ttaactgatc aagcgccagc cctgcgagat aatcattgag cgcaaacatc acctgattgc
     9601 gggcgctgtc catggctttc ggattacgca aaatcaggct gacatcagct ttgggctcgc
     9661 tggcaaaata acggcttggc gcatacacca cgcgcagatt cgactcatca acaatcagct
     9721 ctggatggtc gtatttcttc tctgacttaa tcagcgagaa atcatcagga atataagggt
     9781 taagctctgg caaagagagc gcaatgtcgg cggctttttt ctgccagtcg gcgaaagttt
     9841 gtgcgctgat tttatcgacc tgatacggcg catcgacaaa gtaagccgtt ttgttgtgcg
     9901 gctctttcgg gctgatatac cagatacgcg cattctgcgg cgtcatcatc gccagacgtt
     9961 cctttactgc tttagcatcg taccgatcgg caatattgac tgcatccagc gtatgctcaa
    10021 caggaacgcg aatcatggta tctgccagcc attcgacgta atccatatca cgggtgatcg
    10081 acggataacg gaagtcgata tccagcacat tcgccagttc atcgaagtat tgtttatcaa
    10141 tgcctttttc acgtaacaga ttgagatagc taaaaattgc cgccacaacc tgatcgcgat
    10201 tagccaggcc tttatcagtt aaagacgcag agatcgctaa tacgccgctg ttgccgttga
    10261 cgataggatc ggagttggcg ctaatgccct caactaatcc ctgcttttgc agccagtcag
    10321 aaagtgtacc tgggctgcga ttgccaatca gataggtaat caattcatcg gttttactac
    10381 ggaacttcgc tgagttgtta tcgatgcgaa actcaacgcg caacacttta cgcggcagcg
    10441 cagggacgta atgaatgata atgccctttt gcgcgtcggt gactaccggc acggtgattt
    10501 ccgttttttt gctctctttg ttcggcacgc gaccaaaggt gtccgccgcc atttttgcca
    10561 actccggcag cggtttatta ctgtaaataa ccgccttcat caaattggcg gaatagtact
    10621 tctcgtggaa atctttcagc gcctgctgca ccggattacc aggtttgtcg cttaaagttt
    10681 cgaggttacc accagaaaac tttgaaccgg ggtgtgccgg gttaatggtt tctgcactga
    10741 cctgtgccat gcgcatcccg tcacgcgtac gcgccatggt taattcagcg ttcactgcat
    10801 tacgctcacg ttcggcgtat ttcttgtcga gcaagggttc tgcaatagca tcggccaggc
    10861 ggtctaccgc accgggcaag gcgtcgttct caacttccag atagaaagcc gtgcgatacg
    10921 gcgcagtgct ggcattgtga ctaccgccgt gcattttgag atattcggcc agactgtcag
    10981 cctgcgggta ctttttcgac cccatcagac tcatatgttc aaggtaatgt gccagcccct
    11041 ggtacgcctc gggatcttcc agcgacccaa cgggcactac cagcgccgag agcgatttaa
    11101 ctgcctgcgg atcagaaacc agcaagacca ccataccgtt atccagacgt atagcctgat
    11161 actggcggtt atctttatca cttttacgga tggtttcctg aatcggctgc catcccgttt
    11221 ctgcctgact taatggtgcc cagagggcaa caaacaacaa taatgctttg aaccaggtgc
    11281 tgcggggcat tcacggacct cataatcaac tttattttct ggacagattc aacaggttaa
    11341 cgcaaatcat ctgccagaat ttaatcttgt gctgcacgag tcagcctatg tttatataac
    11401 catcagtccg tgactggtgc gcatcataaa gtaagcggat agattgcgca atttttatac
    11461 aacactcatg actgattaaa gcgaaacagc ggtaacagga aacgttgcga ctgttcaacg
    11521 atggcctcca ttgtctctgg tgttaattgc cgccagagcc gttgatacca gatatcatca
    11581 ccttcgccac gcaccatcat gttgccttcg taagcctgaa ggaatttcgt acgggctttt
    11641 tgcaacgtgg aatcgtcatc cagcatggca tcgttttgcg cgtcataaca ggtttttagc
    11701 cacgcgccgc cactttcagg taacaccagc aatggcgcgg acattccttc acgatacccc
    11761 tcaatcagtt gtgagaggta atgcaaagcc tgttcggctg caagcggcgg aaaacgccac
    11821 tcaccgtctt tgcgtagaaa aaggcgactt tcgccattac caccgctggc acagtagaca
    11881 aggtgttcca gccaaagttg cattccctgc gccacactta ataaagaggg acgccagcgc
    11941 aacaggccat ccggctgcac ctgcggcaac cagccagtta tctgcacacc gttgcaggcg
    12001 agatcaattt ccatactctg ccccggctgg cgacaggcaa tgactctgtc ggcaagctgc
    12061 tgcatctcct ggcactgtgt ttcccagaaa atttcaccaa aagcgccata cggtaaatcc
    12121 cctgccgccc ggaagcggcg gaacaagcgt tcggcatcat cctgctcaac cagtgcattc
    12181 aataactgct gattgatttg ataacggcta agtccttcca gaataaatgg ctcggtgtct
    12241 gggatttcgc tgtcttcagt acggaagttc acctgcaaac gcatctggaa gaacgcccgc
    12301 accggatgtg cccagaatcg ttgtagcgtt tccagcggca cggtttccgg taaggtaaac
    12361 ggcagcggct gaacaaattc agaatgtgct ttaccagcct ggctggccgc aggtagccat
    12421 tcacgagcat agctttgtcg ttcgcctggc tggtagtttt gcggatcaaa cggcatccgg
    12481 gtatggaggc aagtaagatg cgcttttacc cttgcctcgc tttcatcaca gttgagcgct
    12541 tcatcgcccg gtagataatg actttgcccg atgtagtcga tcagttcctg caccagtacc
    12601 gacgggaaac gttcactgtt atcctgaatg gaacgcccga tatagctgat atagagtttt
    12661 tgctgcgcgg aaattaacgc ttccaggaac aggtagcggt cgtcatcgcg acggctacgg
    12721 tcgccacgct tcggtttctg gctcatcagg tcaaagccca atggcgcaag ctgacgtgga
    12781 taaacgccgt cgttcattcc cagcaggcaa accactttga acggaattga acgcattggc
    12841 atcagagtac aaatattaac cggtccggcg agaaaacgct ggctgatacg ttcttgatcc
    12901 agacgctgtg ccagttcatc acgcaatagt gacagcggca ccgcgtcgcc atactgcgca
    12961 cctaaacctt cggcgataat cgcctgccat tgttgttcga tcagcgtcat cgccgcttcg
    13021 gtttccgcat ccggcaggaa gaaggcgttg agcatatcgc gacaaaccgg caaccactct
    13081 tctagcggac gctcctgtgc cagcccgcga cgccagatgt ttagctgcat tagcagtgaa
    13141 gccagatgcc ccaccagttc tgcaattaag ccgctcgatt catcataagg tagaaccgat
    13201 tgccactcgc cctgcgcgct ctccatcgcg tagcccagca acatacgcgt caggccaaat
    13261 cgccaggtgt gttgtccggt ggcggggagt tccagctcgc gaacgttgtc gtcatctatc
    13321 ccccaacgaa tgccggattc gttgacccac tggcgtaaat aacgcagccc ttcttcggtg
    13381 atgtcaaacc gcgccgccag caccggcaca tccagcagcg ccagcacatc ctctgacaca
    13441 aagcgactgt caggcagtga taacaggctg ataaacgctt ccagtaccgg atgtgactgc
    13501 cgcgcacgac ggtcggaaat ggcgtaaggc aggtaacgat ccgcaggtgc gctaccaaac
    13561 actgcctgaa taaacggact gtagctgtcg atatcagcca ccatcacgat gatgtcgcgc
    13621 ggagtaagtg tcggggcttc ctccagcatc gccagcaagc gatcgtgtaa aacttcaact
    13681 tcacgctgcg ggctatggca aacgtggaat gtgatactgc tatccagtgg atcaagcggg
    13741 cgtttgttat cgctacggga aaactcttcg atgttcacac ccgcaacggc gcggttttcc
    13801 agttccagaa tgtcagactg aatgttatgc agcaggttat ctggcgtcac atcgacaaaa
    13861 gcgtccagct cctggctgct ctccaggtca gaaaggagat aaatgtagtc gcgcccaagc
    13921 ttcccccatg aggccagcag cgggttgccg acatcctgtt caccatcgct gttaaagagc
    13981 tgtccggcat tttcgctatc gcgaaatagc ggcaattcgc gatcttcaaa actgtgtcga
    14041 cgctgacgag tcagcagttt cgccagataa gcaggatctt taatatcgcc ccagtaataa
    14101 cggcaggggt tggtaaacag gagatggatt tcaatatgtt tacccagcgc ctggagcgcc
    14161 tggagataaa caggcggtaa cgcggaaata ccgcatataa agacgcgcga aggtaacccc
    14221 ggcgggcagg tcgtcgcgga ctccagcgtt tcgataaagc gctgatagag attcgcgcgg
    14281 tgccagcgcg gttgtccgag ttcatgggta tattccacca gcgccttcca caacggggct
    14341 tgccaggcct gtgcttctcc cagcccttca accaaatgtc ctgtttccca ctgtgccagc
    14401 cagtccggac gatagaccag atactggtca aacaggtccg ccgcttttga ggaaagctgg
    14461 aacagttttc gcttgtcgct atcgtcagtc agataatgcc gcaacagggt aaagtcttcg
    14521 cgctccagca attgcggcag cagagtcatc agtttccagc tcatgctctg tttgttaaag
    14581 gcgctctctt tggggatttc cggtaacacc cggacgaaca tatcccagat aaagctcgct
    14641 ggcagcggaa aatcaatgtt tgccgcaata ccaaactttt gcgacagggt catttgcagc
    14701 cactgcgcca taccggtact ttgcaccaga atcatctctg gttcgaaagg atcgtccagc
    14761 cgttcgcgtt cgacaataaa ctccatcaac gcttccagca cgtccagacg attggaatgg
    14821 tagaccctta acatagcggc tcctgactac tgacgattcg ggcaatgcag gcgggtcatc
    14881 tcgccttctc tgcccccggg tgaaactagc gtaacgctga tgctgacaca tcccgcctgc
    14941 gatgtctgca ttcggttgac ctgccagttg gcaggtggcg aaatcgcgcg cagttgcgtt
    15001 tgctgccaac catgccgcca gagctgttgg tactggtttc tgctggcgag actgttcatt
    15061 aatgttcgct ggatacccga taacgcagtg acaatcatca ccatcagcac catcgcaacc
    15121 attacctccg gcaggctaaa gccttgttga ttcttcaggg aagctgacat aatgccccct
    15181 ctttcagcgg acaaaaatcg ctccagccgc gtggcgaaaa aacaattttt ccatcaatga
    15241 cttcgcctgt tcgccacaac gaaacgcctt cataaccggc aatcaataag gcttcattat
    15301 ctgccagtaa acgcaaacaa acctgggcat cggttccagc gtactgcgag cactgaactg
    15361 ctggctgcgt ctgccaggag tgcatttttc cccacgccag cgccgactga acgatggcct
    15421 ggcggcgcaa tgactgactt tccatgctca cgcgagaggc aaaactgcga tcctgctgac
    15481 tcattccttg caatagcaag ctacccaaaa ccagcagcat caggaccaga gccagtgacg
    15541 aaacaccttt ttcgcggttc acaggttgaa tcctgtcacg ctatagctgg catccaccac
    15601 ggtttgcggt tcagacttac tggcagcacg catattaacc gtcaacaccg gcgagaagcc
    15661 gctgacatcc tgacgtacga cctgaaaagt gtcgataatg atggcatccg gattagtgac
    15721 tttatcccag cccttacctt cacaggatgt cgcaccgcgt agcgtttcca gcacatgctc
    15781 cttcagacga aatccaatct ggtcggactc ttttaccggt tcgcgatccc agataccgtt
    15841 actgttcgca tcccactgca caatgataca gtcaccctgt ccgacaattt ccagcccttc
    15901 gccggtacag ctgccatgac aataacccgc cctctggaga tgcttcgcga cggtaaatac
    15961 ccgcagccag atttcatctt ccagtgccag cttacgagtg ctcgttaaac tttcacgctg
    16021 taacgcaggc agaaagcgtg cagcccccag caacaatacg ctactgatcg ccatagcaat
    16081 caacacttcc agcagagaaa aaccttgctc ttttacaggc atccttctgt ttctccttgc
    16141 tgataaagcc ggagtcttcc ccacggcgaa accaccagcc accactcgcc cgttgagttt
    16201 ttgaagcgaa tatgcccggc ccatgcggta ttgcgcaggc caaagaaagc aagcgaaggt
    16261 gtcaggtcgc tcatttcgac ttcgggccag cgtggcacaa agaccaatgg tgaactgcca
    16321 tgacaggtat tggccccagc agcggaactc acaaggcacc ataacgtccc ctccctgata
    16381 acgctgatac tgtggtcgcg gttatgccag ttggcatctt cacgtaaata gagcaaatag
    16441 tcccgcgcct agctggcggt ttgccaaagc cgttgcgact gctgccagta ttgccagcca
    16501 tagagtccac ttgcgcttag catgaccaaa atcagcatcg cgaccagcgt ttcaatcagc
    16561 gtataaccac gttgtgtttt catgccggca gtatggagcg aggagaaaaa aagacgaggg
    16621 ccagtttcta tttcttcggc gcatcttccg gactatttac gccgttgcag gacgttgcaa
    16681 aatttcggga aggcgtctcg aagaatttaa cggagggtaa aaaaaccgac gcacactggc
    16741 gtcggctctg gcagtatgtt tcgtaattag atagccaccg gcgctttaat gcccggatgt
    16801 ggatcgtagc cttcaatctc aaagtcttcg aaacggtagt cgaagatgga ttcgggttta
    16861 cgtttgataa tcaacttcgg cagcggacgc ggttcgcggc ttaattgcag atgagtttga
    16921 tccatatggt tgctgtacag gtgcgtatcg ccaccggtcc agacaaaatc acccacttcc
    16981 agatcgcact gctgcgccat catatgcacc agtaacgcgt agctggcaat gttgaacggc
    17041 aggccgagga agacgtcaca ggagcgctga taaagctggc aagagagttt gccgtctgcc
    17101 acatagaact ggaagaatgc atggcacggt gccagcgcca ttttatccag ttcgcctacg
    17161 ttccacgctg aaacaataat gcggcgcgaa tccgggtcgt ttttcagctg gttcagtacc
    17221 gtagtaatct ggtcaatatg acgaccatct ggcgttggcc aggcgcgcca ctgtttacca
    17281 tacactggcc cgaggtcgcc attttcatcg gcccattcgt cccagatggt gacattgttt
    17341 tcgtgtagat aagcaatgtt agtatcgccc tgcaggaacc acagcagttc gtggatgatg
    17401 gaacgcaggt ggcaacgttt ggttgtcacc agcgggaatc catcctgcag gttaaaacgc
    17461 atctgatgac caaaaatgga aagcgttccg gttccggtac ggtcgttttt ctgtgtgcct
    17521 tcgtcgagca ctttttgcat cagttctaaa tactgtttca tggttcctca ggaaacgtgt
    17581 tgctgtgggc tgcgacgata tgcccagacc atcatgatca cacccgcgac aatcatcggg
    17641 atggaaagaa tttgccccat gctgatgtac tgcacccagg caccggtaaa ctgcgcgtcg
    17701 ggctggcgga aaaactcaac aatgatgcga aacgcgccgt agccaatcag gaacaagcct
    17761 gagacagctc ccattgggcg cggtttacga atatacaggt tgaggataat aaacagcacc
    17821 acaccttcca gcagcagttc gtaaagctgt gatgggtggc gcggcagcac accgtaagtg
    17881 tcgaaaatgg attgccactg cgggttggtt tgcagcagca aaatatcttc tgtacgggag
    17941 cctgggaaca gcatggcaaa cgggaagttc gggtcaacgc ggccccacaa ttcaccgtta
    18001 ataaagttgc ccagacgccc ggcaccaaga ccaaacggaa tgagtggtgc gataaaatca
    18061 gagacctgga agaaggaacg tttagtacgg cgggcgaaga taatcatcac cacgataacg
    18121 ccaatcaggc cgccgtggaa agacatgccg ccgtcccaga cacggaacag atacagcgga
    18181 tcggccataa actgcgggaa attgtagaac agaacataac caatacgtcc cccgaggaag
    18241 acgccgagga agcccgcata gagtaagttt tcaacttcat ttttggtcca gccactgccc
    18301 ggacgattcg cccgacgtgt tgccagccac attgcaaaaa tgaaacccac cagatacatc
    18361 aggccgtacc agtgaagcgc cacgggtcct attgagaaaa tgaccggatc aaactccgga
    18421 aaatgcagat agctactggt catctgtcac cacaagttct tgttatttcg ctgaaagaga
    18481 acagcgattg aaatgcgcgc cgcaggtttc aggcgctcca aaggtgcgaa taatagcaca
    18541 aggggacctg gctggttgcc ggataccgtt aaaagatatg tatatgatcc gcgctataac
    18601 cctccgcgaa tcagcccgcc catgccgcga cgctccataa aggctgcaac ctgatgacga
    18661 acttcggtcg ccagttgcgc ttccagacta cgctgcgcaa gattttctgc ttcggcaaaa
    18721 tcaatgcgcc gcagcaggta ttttacccgc gctacagaac gtccgttcat agacagatgg
    18781 cgatacccaa gcccaatgag gattgccacg cacatgggat cgcccgccat ttcaccgcac
    18841 aaacggagat cgataccatg tatttccgct tcccgggcga tcatcgccag agctcgtaac
    18901 attgcaggat gaagactgtc gtaaatgttc gccacccggg tattgttgcg atcaacggcc
    18961 agaatgtatt gagtcagatc gttggtgcca acagagatga aatcgacccg ctttgccaga
    19021 tgcggcagca taaataccat tgacggcact tccagcatga tgccgatacg tggtttggga
    19081 atttcgtaac cgatcatctc ctcgacttca cgtccggcac gttcaatcag gcggcgtgct
    19141 tcgtcaactt catcgaggct ggtgaccatc ggcaacagaa tattcaggtt gcccgtagcg
    19201 gcattagcac gcagcatcgc ccgcacctgg atcaagaaga tctccggctg atcgagcgta
    19261 atgcgaatac cacgccaacc caggcatgga ttctcttcgc tgatcggcat gtaaggcagc
    19321 tgcttatctg ctccgacatc cagcgtacgc aaggtgacgg gtttatcatt gaacatttgc
    19381 agcatcccct gatactgcgc cacctgttct tcttccgacg ggaaaccact ttgcagcatg
    19441 aatgggattt cagtgcgata aagaccgatg ccatcaatac ggctgcccag tttttcttca
    19501 tgttccgggc ttaaaccagc attgagcatg acttttatac gctcaccgct ttttaactgc
    19561 gcgggtaaat tgacgtcatc ttccgccaga cggctaagct caatctcttc actaattagc
    19621 cgctgatatt cttgcagcag taccggctcc ggatcgacca gcaattcacc gcgatagcca
    19681 tcaacgatca gcgtcctacg atgcagcacc gaaggctgaa tatccgcgcc catcacggta
    19741 gggatcccca gcgcacgtac catgatcgca gcatgggagt tggctgcgcc atctcgcacg
    19801 acaacaccga ctaagcgatc ctggggcagc tcagcaagag ttgtcgctga cagttcatct
    19861 gccaccagaa tgaaacgttc cggccaggcg ttcggccctt gattagcgtc atcaagatga
    19921 aacagcaatc gctgacccag cgcacgtaaa tcgccagccc gctctttgag atagttatcg
    19981 cttagcgcgg caaactgttc ggcaaatttt tcaatgaccg tttttaccgc ccactctgcc
    20041 accaagcctt tatcaacctc ggcaaacaat tcgcgacgca gccgggtgtc cgaaagcagg
    20101 tgcgagtaaa gatcgaaaat agccgccatt tctttttgtg ctccggcggc aaagcgtttg
    20161 ctgtagcggc gaaactcgtt tgccgcttct tccagcgccc cggtcagtcg ttcgcgttcc
    20221 agagccggat ccagcgttga tgcctgatac acctgttcca ttaaaggtaa cgtggcatcc
    20281 tgccagcctt cggcaatcgc cacaccaggt gctgccggta atgcgcggat tcgcgtctgg
    20341 cgatattgcc caaacagggc agtcaactgc gactgagaaa gaatagctgc catctgggtg
    20401 gcaagcgtca ccaggaagga ttcttcactt tcgtcatact ggcgcaactc tcgttgctgt
    20461 accaccagta caccaagcaa ctggcgacgt tgaataattg gtacgcctaa aaacgcgcgg
    20521 aaacgttctt cttttatgga ggggatgtat ttgaagctgg ggtgcttttg cgcatctgca
    20581 aggtttatcg gttccgccag cctgccaacc aggccgacga tcccttcatc aaacgcgagc
    20641 gttacagtgc gaccgcgtgg ttttttcagc ccccgggtcg ccatcaggta gtaacaacgt
    20701 cgatcatgat cggccaggta gaccgaacag acctcggtat ccatcgcaag acagatgtcg
    20761 gtaaccagaa tatttaacgc ctcattcagg cgtggtgcgc tggctacctt ttcgactatt
    20821 tcgcgcaggc gagtgagcat aattggcgtg acttaacctc ttttacgtcg ataagcagat
    20881 gcgttttgtg gttttggcgt attttcctgc agtgacatca ccacactcgc gaactctttc
    20941 attaccctac ggtagacatc acgtttaaat gacaccacct gtctgaccgg ataccagtaa
    21001 cttacccatc gccagccgtc aaactctggt gtactgctgg tttgcatatt gatttctgca
    21061 tcgccgctta ccagctgcaa gagaaaccat ttttgttttt ggccgataca aaccggcttc
    21121 gtgtcccaac gcaccaaacg tttcggtaat ttgtagcgca accagttacg cgttgaagca
    21181 aggattcgaa cgtctttgcg gcttaatcct acttcttcaa acaattcacg gtacatcgcc
    21241 tgctctgcgg attctccggg gttgattccg ccttgcggaa attgccagga gtgctgacca
    21301 aatcgccggg cccacattac ctgcccctgg cgattacaaa tcacgatacc tacgtttggg
    21361 cggtagccat cgtcatcaat caccggacta cctcaaaata aagctttata tacgaatgat
    21421 tgtttcatac tccaggaaga cggtaaacca ctctctgcag ggcattacac actaataaca
    21481 attgaataac tcacagttat gtgcagagtt ataaacagag gaaggggtgg atagccgttt
    21541 ttattcacct tttctgtgga tagagttgtg aagaactacg gaattactac gggaaaaccc
    21601 ggagcattct gaataagccc gatatagaca ttttaaaata ttcattatta gtcaatgcat
    21661 tacatgtaat ttgagtaatg aaaaagagta attcgtgacc caggtcacac ctctcattta
    21721 cgggttggcg aaagatcaac caatgccgta tttatccaca gaatgtgcca ctaagttaag
    21781 cactgaacca ctaaaaactg gagtttcgtc gcacgtcaag gctgtaaatg aaaacagtag
    21841 tggaggtttt tcacagttat cccagctttc tgtggataac atggtgtaag atcctgttta
    21901 ttttcagtga ccagatttgg aaaacctatt gcagtgttgc gcaactcgat taccggcagc
    21961 ctaaaaagca atataaatca gagaattgaa caacacatgt ggaaaaagtt acactgcgaa
    22021 tattcggcac ataattgctg tttgtttttt aaccaaggca tcatgacatg tcccaacctc
    22081 gcccactgct ctctcctccc gaaactgaag aacagttgtt agcgcaagca cagcaacttt
    22141 ctggttatac attgggagaa ctggcggcac ttgccgggct ggttacgcca gagaatttaa
    22201 aacgcgataa gggctggatt ggcgtgttac tggagatctg gctaggtgcc agcgcaggga
    22261 gtaaacctga gcaagatttt gctgctctgg gcgtggaact taaaactatc cctgtggata
    22321 gtcttggtcg tccgctggaa acaacattcg tttgtgttgc cccgttaacg ggtaatagcg
    22381 gggtgacctg ggaaaccagc catgtgcgcc acaagctcaa acgcgtactg tggataccgg
    22441 ttgaaggcga gcgcagcatc ccgctggcgc agcgtcgcgt aggatcaccg ttgctgtgga
    22501 gcccgaatga agaggaagac cgacagctac gcgaagactg ggaagaatta atggatatga
    22561 ttgttctcgg tcaggttgag cggatcaccg ctcgtcacgg ggaatattta cagataagac
    22621 cgaaagcagc gaatgcgaaa gcgcttaccg aagccattgg tgcccggggc gaacggattc
    22681 tgacgctgcc acgcggcttt tatttgaaga agaatttcac cagtgcacta ctggcccgtc
    22741 attttctgat ccagtagcca tcgctttgac ctgccgcttt ccgggcatat aattaccgct
    22801 tcattttttt ggcagggctt tttagatgtt atttgcatgg ataaccgatc ctaacgcctg
    22861 gcttgcgctc ggtacgctga cgctgctgga gatcgttctt gggatcgaca atattatttt
    22921 cctttctctg gtggtggcaa agcttcccac agcacaacgt gctcatgcgc gccgtctggg
    22981 gttggcggga gccatggtta tgcgtctggc gctgctggca tcaatctctt gggttacgcg
    23041 cctgacaaat ccgcttttta caatattcag tcaggaaatt tctgcccgtg atttgattct
    23101 gcttctgggt ggcttgttcc ttatctggaa agccagcaag gaaatccacg aatccattga
    23161 aggtgaagaa gaagggctga aaacacgcgt ttcatcattc ctcggcgcta tcgtgcagat
    23221 tatgctgctg gatattatct tcagcctcga ctcggtgatt accgctgtgg gtctgtcaga
    23281 tcacctgttt attatgatgg cagccgtggt gattgccgta ggcgtgatga tgttcgccgc
    23341 gcgctcgatt ggtgattttg tcgaacgcca tccttcggta aaaatgctgg cgctctcttt
    23401 cctgattctg gtgggcttta ccctgattct ggaaagtttc gacatccacg taccgaaagg
    23461 ttacatctac ttcgcgatgt tcttctctat tgcggttgaa agcctcaact tgattcgcaa
    23521 caaaaagaat ccgctctgat actccgtacg ctctcctgcg ggagggcgta ctcactcctt
    23581 ttaccctcac agtttaagat tttcctgctt tcaagatata taacgtcgat ttataaacag
    23641 actattatca taggtaggcc aaatgaggat aaccggatga aaaaatgggc agtaataatt
    23701 tctgcagtcg gactggcgtt tgctgtttcc gggtgttcca gtgattacgt catggcgacc
    23761 aaagatggcc gtatgatttt gaccgatgga aaacctgaaa ttgatgatga taccgggctg
    23821 gtgagttatc acgatcagca aggtaacgcg atgcaaatta accgtgatga tgtttcgcaa
    23881 attattgaac gttaacaaat aaggtcagca tccggctggc cttaagattt ttctctttcc
    23941 cttttccctt ccctctgcca tttttatatt ccttatgtcg tgattataaa aaggaaacgg
    24001 ctatgcaata tcaccgtata ccccacagtt cgctggaagt cagcacgctg gggcttggca
    24061 cgatgacgtt tggtgaacag aacagcgaag ccgacgccca cgcacaactc gactatgccg
    24121 tcgctcaggg cattaacctt atcgacgttg ccgaaatgta cccagtacct ccgcgccccg
    24181 aaacgcaagg gttaaccgaa acctacgtcg gcaactggct ggcgaaacat ggcagccgcg
    24241 aaaagttaat tatcgcctcc aaagtgagcg gaccgtcgcg caataatgac aagggcatcc
    24301 gcccggatca ggcgctggat cggaagaata tccgcgaagc gctgcatgac agcctcaagc
    24361 gcctacagac tgattacctc gatctttatc aggtgcactg gccgcagcgc ccgaccaact
    24421 gcttcggcaa actcggttat agctggacgg attctgcgcc tgcggtttcg ctgctggata
    24481 cgctggacgc actggcagag taccaacgcg cgggaaaaat tcgttatatc ggcgtgtcga
    24541 acgaaactgc atttggcgta atgcgctacc tgcatctggc ggacaaacac gatctgccgc
    24601 gtattgtcac cattcagaac ccttacagtc tgttaaaccg cagttttgaa gtaggtctgg
    24661 cagaagtcag ccagtatgaa ggggtcgaac tgctggccta ttcgtgcctg ggtttcggca
    24721 cgctgaccgg gaaatatctc aatggtgcaa aacccgctgg cgcacgtaat acgctcttta
    24781 gtcggttcac ccgctatagc ggtgagcaaa cgcaaaaagc cgtcgcggcg tatgttgata
    24841 tcgccagacg tcatggcctt gatcctgctc agatggcgct cgcgtttgtt cgccgtcaac
    24901 cgtttgttgc cagcactctg ctgggcgcaa ccacgatgga tcagctgaaa actaacatcg
    24961 aaagtttgca tctggagtta agcgaagacg tattagctga aattgaagcg gtacatcagg
    25021 tttatactta tcctgcacca taaagattaa cgccggttaa aaccggcgtt aaatattaat
    25081 gacggcgctg ccagatccac agcgccgtta ttgccagcgc aaatagcgca ccgaagccaa
    25141 tgccaatggg cacgaccggg atgcctacca ttaccgccag cgagtaaatg cccagcatca
    25201 acaacatggc gctgttttcg ccaaggtttt gtactgcaat cgcattcccc gccccgacgc
    25261 tttttttacc ccgctcctgt agcaacgcat tgagcggaac gacaaaaaag ccccccagca
    25321 cgccaatcag catcaacaag gcataggctg gcagcagctc gtgttgcagg gagaaaatca
    25381 gtaccaccac gccaatcaaa atcccggctg gcatacagcg tgacacggtt tccagcgtaa
    25441 ccagcttcgc tgccgcacct gcgccaacca cgataccaat cgctaccatc gcgttgagat
    25501 aggtcggcgt agcgttatcg gtaatgccca gcgccaccgg tacccacagc accaacagga
    25561 aacgcagcgt gacacccgct ccccagaata aactggtgcc caccagcgaa aaacgcgttt
    25621 caccattgca ccatagcgag gtgcaggcat tcaggaaact gcgggtcatg ttgatgagat
    25681 tccaggactg ccccggacgc gccgccgcca gtttgggaat gtagatattg gcaacgaccg
    25741 caccaccgta ggccagtacg catgcggcca gggcgaccag gacatgccag tcagccagca
    25801 caccaccggc tacggaaccg agtaaaatcg ccgctatggc agaagcttcc attaaaccgt
    25861 tagcttttac taacttacta cccgtggtta attcgccgag aataccgtat ttcgccggtg
    25921 aataggctgc agcaccaaca cccaccagcg tatagccgag aaacggattg ataccaaagc
    25981 agatactggc tgcgcccagc agcttcaggc cgttggcaaa catcatcacc cggcctttgg
    26041 cgaagctatc cgccacctgc ccgacaaacg gcgccaaaag aatgtaagca cctacaaaca
    26101 ccatttgcag gatgggctgg ctccactccg gacagaactg cgctttcagt aacgccagag
    26161 tggcaaacag tagcgcgtta tcgccaaacg cagagagaaa ctgcgccacg ataaccgctt
    26221 tcatcccctt cgaccacaac gaagtgttag tgtgcactga ctcactcatc gtgttgttcc
    26281 gcttcgtcta cccagctttt caacgtgaca aagtcaggtt tgccgctgcc aagtaatggc
    26341 atctgtttca gatagcgaat atcgcgcggt acagcaagct ccggcacgcc gtgctcgcgg
    26401 gcatactgtt gcaacttatc gcgcgtcagt tcgttatctg tggtgaaaag caccagtgcc
    26461 tcgcctttgc tggcatcgct cttaatcgca gtggcatgga ctttatctgg cgaaacacca
    26521 agtgccagtt gttccaccat ttccagcgac accatttcgc ctgcaatttt ggcaaagcgt
    26581 tttgcgcggc cctgaatctg cacaaagccc tgctcgtcaa aacgcacaat atcgccagtg
    26641 tcataccagc cgcgctccat ttcgccgcga acattctcgg cggtgggcac ttccagtaca
    26701 cctggcttct ccacccgcag atagccgttc attatgttcg gccctttcag ttgcaggcgt
    26761 ccgccctctt cgataccagg gaccgacaac aggcgtgcat ccattcctgg tagaatacgc
    26821 cctaccgtac cgggtttcgc cgccatcggt acgttgatag aaacgacagg cgcgcattcg
    26881 gtcacaccgt agccttcaag gatgcgcagg ccaaatttat cctgccaaag ctgtttggta
    26941 ctttcttgta gtttttctgc gcctgccacc acatagcgta gacgatagaa gtcatacggg
    27001 ttggcgaaac gcgcgtagtg accgaggaaa gtcgaggtac cgaacaacac ggtgcaactg
    27061 cggtcataca ccaagtccgg cacaatgcgg taatgcagcg ggcttggata aaggaacact
    27121 tctgcacctg taagcagtgg cgtaaacagg cctaccgtca gcccaaagga gtgaaacagc
    27181 ggtaatgccg acataaagcg atcgttggtg gtgaagtcgg caatcgtttt aatctgctcg
    27241 acgttcgcca gaatgctttt atggctatgg acgacgcctt ttggatggcc ttcagaaccg
    27301 gaggtaaaga ggatcagcgc ctcttcttcc ggctgctgtt ttacctgcgc caggcgcggc
    27361 atcagcaaat gagcgaagat ccatactttg tcggcagtgg tgacatctgc ttttaaatct
    27421 tccagataga cccagcgcac ctgagtaagt tgctccggca gatgccagag tttgccttta
    27481 tcgagaaact ggcgggaagt gaagatggtt ttgatttcag ccgccgtaat aacactggtc
    27541 agccctttta ccccggcagt gtagttcatc attgcgggaa tgcggcgacg cgcgatggcc
    27601 ccaaaaatca ctgccgcact gatgcctgca ttgggcagca ttaagccaat gcgttcgcct
    27661 tcaacactgt atttttcaag gatgcgtcca acaaacagcg ttttcgtaag caatttgcga
    27721 taggagtctg gggtaaagtt gacgtcttcg acacatttct tcccggctcc gaagcggtac
    27781 attgcactca gtaaagattc gtacagagtt tcacgcgggc gcaccgccat acgcgcttcc
    27841 atcattattt gatgcagcat ttcgccagcg attttgcgac ggtcacgggc acgcggcgca
    27901 tccggcatcg ccacctgcgt tggaggcaaa atatgcagag taatttgcgg gaacaagcga
    27961 cgtttaacca gacctttcag gcggctgaag tgcgtaagtt ccgccccttc aatacgcaca
    28021 ggaataaccg ttgcaccaga cttcgccgcg acaaaacccg cgccatcgta gattttcatc
    28081 agcgagcctg tagtggtgat gcgtccttca gggaaaatca ccactggtcg gccctgttcc
    28141 accagacgta ccagatgttt aatagccata ggttgcgtcg ggtcgagagg aacaaagtcg
    28201 ataaatgatt tcagccaacg catataccac tgttggctta ttgaggtgta aacggcaaac
    28261 actggacgca caggtaaaaa cagtccaagc aaaatgccat caataaaaga gacgtgatta
    28321 ggcgtaatta gaacgcgctc gcccttcagt gcctgggtgt cacccgtaac gcgaacgcga
    28381 tacaaaacac ggcacaaatt tcgaaaaaag ctaaaaagca ttccaactcc ctttgctctg
    28441 attcagtaaa agcgaatgga gggagattac acgagataaa gaacgcgagc gacagtaaat
    28501 taggtgcgaa aaaaaacctg cgcatccgcg caggttggtg caagagacag ggtacgaaga
    28561 gcgtaccgaa taatctcacc aatcaatacc tctgggatca ccactttagc aacctgaagc
    28621 caaacgccac cagcggtcaa tcgcaacagc gtttcgcaaa gtgtaaccaa aggtttgcaa
    28681 tctctttttt tgtcttactt atttgcgttt ggctcacatt cccacgatga aaacacgcca
    28741 ccccttgaac caacgggcgt tttccgtaac actgaaacaa tgtaagcgtt tacccactaa
    28801 ggtattttca tggcgaccat aaaggatgta gcccgactgg caggcgtttc agtcgccacc
    28861 gtttcccgcg tcattaataa ttcacccaaa gccagcgaag cttcccggct ggctgtgcat
    28921 agtgcaatgg agtctcttag ctatcacccg aacgccaacg cccgtgcgct ggcgcagcag
    28981 accactgaaa cggtcggtct gatcgttggt gatgtttccg atccgttttt cggtgcaatg
    29041 gtgaaagcgg tcgaacaggt ggcttatcac accggtaatt tcttattgat tggcaacggt
    29101 taccacaacg aacaaaaaga gcgtcaggct attgagcaac tgatccgcca tcgctgtgct
    29161 gcgttggtcg tccatgccaa aatgatcccg gatgccgatt tagcctcatt aatgaaacaa
    29221 atgcccggta tggtgctgat caaccgtatc ctgcctggct ttgaaaaccg ttgtattgct
    29281 cttgacgatc gttacggtgc ctggctggca acgcgtcatt taattcagca aggtcatacc
    29341 cgcattggtt atctgtgctc taaccactct atttctgacg ccgaagatcg tctgcaaggg
    29401 tattacgatg cccttgctga aagtggtatt ccggccaatg atcggctggt gacatttggc
    29461 gaaccagacg aaaacggcgg cgaacaggca atgaccgagc ttttgggacg aggaagaaat
    29521 ttcactgcgg tagcctgtta taacgattca atggcggcgg gcgcgatggg cgttctcaat
    29581 gataatggta ttgatgtacc gggtgagatt tcgttaattg gctttgatga tgtgctggtg
    29641 tcacgctatg tgcgtccgcg cctgaccacc gtgcgttacc caatcgtaac gatggcgacc
    29701 caggctgccg aactggcttt ggcactggcg gataatcgcc ctctcccgga aattactaat
    29761 gtatttagtc cgacgctggt acgtcgtcat tcagtgtcaa ctccgtcgct ggaggcaagt
    29821 catcatgcaa ccagcgacta accgcagtta aagcaattcc agcgccagta attcttcgat
    29881 ggtctggcga cggcgaatca accgcgcctg accattatca aacagaactt ctggtaacag
    29941 cggacggcta ttgtagttgg atgacattga tgcgccatat gcccctgtat catgcagtac
    30001 cagataatca cctgccttca cttccggcaa ggcgcgggtt tcaacatttc ccccttcctg
    30061 ctgggtaaag acatcgcccg attcacataa cggcccggcg acgacggttt ccaccgttgg
    30121 cgcgtgttcc agagaacgac catcagctgc cagggcactg atatggtggt aactaccgta
    30181 cattgccggg cgcatcagat cgttgaaccc ggcatcaacc agcacaaagt ggcggctacc
    30241 catttgtttg acgctccgca cctgagtaat taatacgcca gactgcgcta ccaggaagcg
    30301 acccggttca atttccagtt tcacagggtg gcccaaatgg cgagcgattt gctcacgcgc
    30361 ggcattccac agaccataat aatgttcggt atcaaccgcc tcttcaccct gttgataagg
    30421 aatagaaagc ccaccgcccg cagaaatagc ctgtaaatcc tgaccgaatt cgatgacctg
    30481 acgcaccata gcaccacaca cctgctccag atgggcataa tcaacgccag aaccaatgtg
    30541 catgtgaatg ccgactagct gcagatgatg acgttgtatc acgtccagtg cggcgggcag
    30601 atcggtgtac cagataccgt gcttgctgtt ttcgccaccg gtattggttt tttggctatg
    30661 tccgtgacca aaccccggat taacgcgcag ccatacccga tgccctggcg aaacctggcc
    30721 cagttggtcg agcatatcaa cagaacccgc attcaccgga atttgcaatt cactgacgcg
    30781 ttcaagcgtc gcctgatcga taacatctgc cgtaaaaaca atatcatcgg ggtgcgtttg
    30841 cggattgtaa cccgccgcca acgcacgctc tatttcgcct aacgagacgg aatccacttt
    30901 cacgccctgc tcacgcatta agcgcaaaat atgaatattg gaacaggctt tctgtgcaaa
    30961 gcgcaccact tcaaactgtt tcagcgctgc aatctgccga cgaataattt gcgcatcgta
    31021 gacccacacc gggcagccaa attcagcggg caaacgcagc agattttcgg cggtgagatc
    31081 ggtatcggtg ctgaacagtg aatgtggcat aacaaactcc agatgagtgc ttttttatga
    31141 ttacgccaca tcataaaaag aataaaaaat atcgatttat gtcgaatcta tgcaaaaatg
    31201 atatggatta ccggattgcg agagagcgct aatggccgcc gttaacttac gtcatattga
    31261 aatttttcat gcggtaatga ccgccggaag cctgactgag gcagcacacc tgctacacac
    31321 ctcacagcca accgtcagcc gcgaactggc gcgctttgag aaggtgatcg ggctgaaatt
    31381 gtttgagcgc gtacgtggac gattacatcc taccgtgcaa ggactgcgtc tgtttgaaga
    31441 agtgcaacga tcctggtacg gactggatcg cattgtcagc gccgcagaaa gtctgcgcga
    31501 gtttcgccag ggagaactgt ctattgcctg cctgccggtc ttttcgcaat cttttttacc
    31561 gcagctcctg caaccctttc tggcacgtta tcccgatgtc agcttaaata tcgtgcccca
    31621 ggaatcaccg ctacttgaag agtggctctc ggcccagcgt catgatttag gactcactga
    31681 gacactccat acgcctgcgg gaacagaacg taccgaatta ctctctttag atgaagtgtg
    31741 tgtgttacct ccgggtcatc cgctggcggt aaaaaaggta ttaacgccgg atgattttca
    31801 gggtgagaat tacatcagcc tttcccgtac tgacagctat cgccagttgc tggatcagct
    31861 atttactgaa catcaggtta aacgacgcat gatcgtagaa acccacagcg ccgcgtcagt
    31921 ctgcgcaatg gtacgggcgg gggtaggtgt ttcggtggtt aacccgctca ccgcactaga
    31981 ttatgcggca agcggtttag tggtgcggcg gttcagtatt gcggttccgt tcaccgtcag
    32041 cctgatccgc cccctgcacc gcccgtcatc agcgctggtt caggcgttta gtgggcattt
    32101 acaagcgggg ttaccgaaac tggtcacttc tcttgacgct attttgtcgt cagctacgac
    32161 agcataaaag cgacagcatc ctcggcatgg atcgccgcgg tatcaaacac aggcaaaaca
    32221 ctgcgctctt ctggcaccag taaaccaatt tctgtgcagc caaaaatgac gccctgtgcg
    32281 ccctgttctg caaggcgagc aatcacttgc gcataataag cgcgtgacgc ttcggtaaat
    32341 ttccccagac acagttcttc aaaaataatc tgattaattt tcgcccgttc atccgcttca
    32401 ggaataagac aattgatgga aaattgttcc gtcagccgcc cgcgataaaa atcctgttcc
    32461 atggtgtaac gcgtacccag cagcgccaca cgagtcattc cggccccggt aattgcacgt
    32521 ccggtggcat ccgcaatgtg taagaaaggc agagtgcaac gtgactcaat ggcatccgcc
    32581 actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg ctgtaagcca
    32641 agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg ctggcactct
    32701 tctatttcat gaaaatcgac gctatgtagc agcatttgcg cagagtgaag cccaccaagc
    32761 cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc ccagctcatt
    32821 cctcccagca aaccaattgt tttcattcct ctctcctgat gtgtgttacc gcaacaaggc
    32881 tgaataagga acgagacaaa cgcctcaacg gccaggtgcc caatctctat taacgaaaaa
    32941 agggccggat gtacagcaca tccggcccgt gaaatcagac gccgatattt ctcaacttct
    33001 cgcctgccat cagtttgcgt tcgatatgtt ccagcgtgac atttttggtt tccggaatga
    33061 gccagaaagt aatgcccaca aacgcaatgt tcaacgcagt gtagagccag aacgtaccgg
    33121 cagcgccaat gctatcaagc agtgtcagga aggccgcgcc gataatcata ttcgacaccc
    33181 agtttgtcgt cgtcgaacag gtaataccga aatcgcggca tttcagcggc tgaatttcag
    33241 agcacaggat ccacaccact ggcgcggcgc tcatcgcata accggcaata cacatcatcg
    33301 tcatgccaac agagagccag gacaagccac tggaagccgt accgttatca aactgcatca
    33361 ggcaatagcc cagcaccaga gtgcctaacg ccatcacgct gaaaccaatt ttcagagccg
    33421 gtttacgacc tgctttatct accgtaaaga ccgcaatgaa ggtcgcgaac ataaaggtca
    33481 gtcccacgac cagagtcgca atcatctgtt gttctgtggt cgtaaagccc gccattttga
    33541 agatacgcgg cgcgtagtac atgatgatgt tcataccggt aaactgctgc atcgcctgca
    33601 acaacatacc gagaaacaca gcacgacgga cgttacggtt gatcttaaac agtgcccaac
    33661 cgccctgttt taacttcagg ctttcacgaa tttcgttgag ttcttctcgc gctttttccg
    33721 acgtatcgcg cagcatacgc aacacttctt ccgcctcaat atgacgcccc ttttccgcca
    33781 gccagcgcgg gctatttggc aggaagacca ccagaataat cagcagaact gctggtaaag
    33841 caagaacccc aacattgcgc gccagttacc gctataactg aacgctgtat cggataaaaa
    33901 cgccagcacg atgccgagtg tgaccatcaa ctggtacata ctgatcatct taccgcgaac
    33961 gtacttgcca tttcagaaag atacagagga gcggtgtaag acgcgatccc gacagcaatg
    34021 cccagcacca cacgagcggc gattaacatc tctacgctgg tcgcaaaagc ggaccctata
    34081 gaaccgagta caaacaggat ggcccccgcc atcaggctgt atttacgccc caggcggaac
    34141 gacagccaac cattaaacag cgcaccaatt gctgcgccga gcatcatgct actaaccacc
    34201 cattcctgca aacgactggt cagcacaaag tgatcggtaa tgaacggcaa cgctccggcg
    34261 attacgccga tatcaagacc aaataacaat cctgcgaccg cagcagctac cgaaacaaac
    34321 atattcatac gccgcgtatc acgcaaagaa cgtggcgtta aagcagattc cgtattgata
    34381 gtaaccattt tttcctgcca gcagagagta agacattgtg aaaaaatacg tgaacaattc
    34441 acacaggtgt caggtcggaa acagcataaa tatggattaa attgctgcga catgccgtta
    34501 tgtgatggat attccaattt taaaattaag ttgaattatt gagattatta ttaaccacct
    34561 aattttaaag tagataaaat tcataaagtt cattaattga taattgatat ggattatttc
    34621 ataaccatga tatggattat gatgatctag agatataaaa aaccctgcca tgcggcaggg
    34681 tcataaaagt aagaggaatg aattaacgcg ccagccaacc gccatccaca gcaatggtat
    34741 aaccatttac ataatctgaa gcgctggagg caagaaacac taccggcccc atcaggtcac
    34801 tcggcagtcc ccaacggcca gctggaatgc gatcgagaat ttccgcgcta cgttgttcat
    34861 ctgcccgcag ttgttgggta ttgttggtcg ccatgtaacc cggagctatc gcattaacat
    34921 taatgttgtg tttagcccat tcgtttgcca tcaatcgcgt cacacccatc actccgcttt
    34981 ttgatgcggt ataagaaggc acacggatcc cgccctggaa ggagagcatt gacgcgatat
    35041 tgataatctt gccgccattg ccttgcgcga taaagtgttt cgccgctgcc tgagacatga
    35101 agaatacgct cttgatattc aggttcatga catcgtccca gtctttttcg ctgaactcga
    35161 gagcgtcttc gcggcgaatc aatccggcgt tattcaccag gatatcaata tgaccaaatt
    35221 cagctaccgc gcgatccaac agtccaggaa tgccatcaat ctttcgcaga tcggcggtca
    35281 ggcttaaaaa acgacgcccc agcgccgtga cctgctcgat ggtttcagtc ggttcaacga
    35341 tgttaatgcc aacaatgtca cagcccgctt gcgccagccc caacgccatc ccctggccca
    35401 gcccagtatc acaaccagtg acgaccgcaa ctttaccttg gagagaaaat gcacttaaaa
    35461 tcataacaac accttattcg tttatgccca caactagcgc aaatctttaa cggccacatg
    35521 gtccatatca ttaaagacct ggttttcacc gaccatgccc cagataaagg tataagcttt
    35581 ggttccgacg ccggaatgga tcgaccagct cggggagatc accgcctgct cgttatgcat
    35641 cacaatgtga cgcgtttctt gcggctgccc catcatgtgg aaaacgcagg cgtcatcatc
    35701 catattgaaa tagaaataaa cttccatccg gcgctcgtgg gtgtgacacg gcatggtgtt
    35761 ccacaagtta cctggagcca gctccgtcag ccccatactc aattgacagg tttccagtac
    35821 atccgggaca aaatatttgt taatcgtgcg acggttactg gtgaggttat cgcctaacgt
    35881 gactggagat acttcgtccg gtgtgacttt tttggtggga tacgtcgtat gcgcgggtgc
    35941 gcaattgtaa taaaacttcg ccggagtggc ggtatcaata ctggcaaaga caacttcttt
    36001 tgcaccttta ccaacataca gggcgtcgcg gtgaccgatt tcatagcatt gaccatcgac
    36061 agtaatcgta ccggcaccgc caatattgat aacacctaac tcgcgacgtt caaggaaata
    36121 gcttacgccg agttgtttac caacttcccc gccaacggaa accgttttag ttatcggcat
    36181 aatgccgcca acaataattc ggtcaatgtg gctgtaaacc atggtgtact catcggcgac
    36241 aaataccttt tcaaccaaaa attcattacg cagcccttgg gtatccagcg tttttgcgtg
    36301 cgcaccgtgg atgctctgtc ttacgtccac atcaacctcc gataatcagt gaagcgtgaa
    36361 aatagaaaac gatagtgaaa cactgtttcg ttttctctat aaagggatga tatgcccatg
    36421 ggctagggtt ttcaattaaa aataaaacaa tgttttattt ttaatttaag gattaaaaaa
    36481 aatgcagtgt cgatcacgaa tatgtcatga gaatggaaaa atgagggagc ccagaaaatg
    36541 tatgtcgact tgccagccat cattattcaa cagattaatg acggcagata tttatcggta
    36601 aggaggaaat gcagagctac tcgtcacgtt caatggtcaa tgccacgccc tagcccccgc
    36661 cgatacaaag cgttgccagc cctttgcggg cattacgttt caccatttca tgaaccagag
    36721 aaaccaggat tcggcaaccg gaagcgccta tcgggtgacc gagtgcgatc gcgccaccat
    36781 tgacattgac ccgacgctca tcccactcaa gcatcttgcc aaccgaaagc gcctgtgcag
    36841 caaacgcttc attagcctcg ataagatcga catcagccaa ctgccagcct acacgctcca
    36901 ggcaacggcg ggtcgcatac accggcgcaa ttcccatcaa tgccggatct acaccaacgc
    36961 tggcaaatgc gcggatgcgg gccagcacgg gtaaattcaa cgcttgtgct ttggcttcgc
    37021 tcatcatcat taccgcagct gcgccatcgt ttatggatga tgcattcccc gcggtcacag
    37081 aaccgagact atcaaatgaa ggatttaaac gggctaaacc ttctgcactg gcgtcagtgc
    37141 gtggctgttc atcggtatca acaaccaacg tctgaccgtt actttggatc attaccggga
    37201 cgatctcatc tttaaatcgt ccggcgtcaa tcgccgctcg cgctttttgt tgcgagctaa
    37261 gtgcgtaagc atcctgcaac tgacggctga tgccatattc gcgagccaga ttttcggcgg
    37321 tgacaccaat atgataatca ttgaaggcat cccacaaccc atcatgcacc agactgtcaa
    37381 ccaactggct attgccaagc tgtgcaccgg tgcggctatc agtcagaaca tgtggtgcgc
    37441 ggctcatgtt ttcctggcca ccggcgatga caatatcagc ctcgccacac tgtatcgcct
    37501 gagtagccag atgcagtgct ttaagaccgg aaccgcaaac gtcattaatc gtgattgcag
    37561 aaacgctgtt aggaagacca cctttaatag ccgattgcct tgccggattc tgccctgccc
    37621 ctgcagtcaa cacctgacca agaattactt catccaccgc atatgcagga acgccggtac
    37681 gttctattaa cgctttcacg accagactac caagttccac ggcggaatga cccgctaacg
    37741 caccacgaaa gcagccgata ggtgtccgta acgccccgac aatcacaacg tctttcatca
    37801 caacctcgca ccaattaaca gtgcaatagt aaatgattgt taattacagt tatcttaatt
    37861 gtttaaaaaa agtgattttt atcacaaagg aaatatgcct gagcagtagt cagagtcata
    37921 actggcacgt aaggtttgca accactaacc caccaataga tgggtagata gggcgttaat
    37981 ctcccatact taacttggtt tatggtaaat tgccctccat tttgtttaat ttgtagatga
    38041 tacgttcaga taatgtctaa tatttggtct aaagaagaaa ctctgtggag tttcgcgctc
    38101 tacggcacag ccgttggtgc aggcacgcta ttccttccta ttcagttagg ttcggcaggg
    38161 gctgtggtcc tgtttattac tgctctggtc gcctggcctt taacatatta gctacataaa
    38221 gccttatgcc agttcatcct ctcatcaaaa acatcagcag gtgaagggat aacgggcgcg
    38281 gtaacacact actatggcaa gaagattggt aatctgatta ccacgctgta cttcatcgcc
    38341 ttttttgtcg tcgtgttgat atatgcagtg gcaattacca actcacttac ggaacagctg
    38401 gcaaagcata tggttattga tcttcgcatc cgtatgttgg tgagtctggg tgttgtatta
    38461 attctgaatc tcatttttct gatgggacgt catgccacta ttcgggtaat gggatttttg
    38521 gtattcccat tgattgccta tttcttattt ctttccatt
//
LOCUS       DADMEQ010000024        37976 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.024, whole genome shotgun sequence.
ACCESSION   DADMEQ010000024 DADMEQ010000000
VERSION     DADMEQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.024"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            1..2005
                     /gene="speF"
                     /locus_tag="JNZ05_001291"
                     /pseudo
     CDS             1..2005
                     /gene="speF"
                     /locus_tag="JNZ05_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            2002..3321
                     /gene="potE"
                     /locus_tag="JNZ05_001292"
     CDS             2002..3321
                     /gene="potE"
                     /locus_tag="JNZ05_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HAY8564588.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGTSLSPVQIGLATIGVQWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLPLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(3377..5017)
                     /gene="pgm"
                     /locus_tag="JNZ05_001293"
     CDS             complement(3377..5017)
                     /gene="pgm"
                     /locus_tag="JNZ05_001293"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HAY8564589.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5043..5588)
                     /gene="seqA"
                     /locus_tag="JNZ05_001294"
     CDS             complement(5043..5588)
                     /gene="seqA"
                     /locus_tag="JNZ05_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HAY8564590.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5773..6537
                     /gene="ybfF"
                     /locus_tag="JNZ05_001295"
     CDS             5773..6537
                     /gene="ybfF"
                     /locus_tag="JNZ05_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY8564591.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6608..6970
                     /gene="ybfE"
                     /locus_tag="JNZ05_001296"
     CDS             6608..6970
                     /gene="ybfE"
                     /locus_tag="JNZ05_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HAY8564592.1"
                     /translation="MYYGALSIRAEAWLIVSPEVTKIMAKEQTDRTTLDLFAHERRPG
                     RPKTNPLSRDEQLRINKRNQLKRDKVRGLKRVELKLNAEAVEALNELAESRNMSRSEL
                     IEEMLMQQLAALRSQGIV"
     gene            7110..7640
                     /gene="fldA"
                     /locus_tag="JNZ05_001297"
     CDS             7110..7640
                     /gene="fldA"
                     /locus_tag="JNZ05_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HAY8564593.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7850..7936
                     /gene="uof"
                     /locus_tag="JNZ05_001298"
     CDS             7850..7936
                     /gene="uof"
                     /locus_tag="JNZ05_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HAY8564594.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7929..8375
                     /gene="fur"
                     /locus_tag="JNZ05_001299"
     CDS             7929..8375
                     /gene="fur"
                     /locus_tag="JNZ05_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HAY8564595.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8459..8785)
                     /locus_tag="JNZ05_001300"
     CDS             complement(8459..8785)
                     /locus_tag="JNZ05_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564596.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVCVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8822..10228)
                     /gene="chiP"
                     /locus_tag="JNZ05_001301"
     CDS             complement(8822..10228)
                     /gene="chiP"
                     /locus_tag="JNZ05_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HAY8564597.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSYFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYNDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(10806..12470)
                     /gene="glnS"
                     /locus_tag="JNZ05_001302"
     CDS             complement(10806..12470)
                     /gene="glnS"
                     /locus_tag="JNZ05_001302"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HAY8564598.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEIGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(12673..14619)
                     /gene="nagE"
                     /locus_tag="JNZ05_001303"
     CDS             complement(12673..14619)
                     /gene="nagE"
                     /locus_tag="JNZ05_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HAY8564599.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVIINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            14952..15752
                     /gene="nagB"
                     /locus_tag="JNZ05_001304"
     CDS             14952..15752
                     /gene="nagB"
                     /locus_tag="JNZ05_001304"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY8564600.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVVNSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            15812..16960
                     /gene="nagA"
                     /locus_tag="JNZ05_001305"
     CDS             15812..16960
                     /gene="nagA"
                     /locus_tag="JNZ05_001305"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY8564601.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPNFKITKTIVNGNEVVTQ"
     gene            16969..18189
                     /gene="nagC"
                     /locus_tag="JNZ05_001306"
                     /gene_synonym="nagR"
     CDS             16969..18189
                     /gene="nagC"
                     /locus_tag="JNZ05_001306"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HAY8564602.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALKERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18237..18979
                     /gene="nagD"
                     /locus_tag="JNZ05_001307"
                     /pseudo
     CDS             18237..18979
                     /gene="nagD"
                     /locus_tag="JNZ05_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            19376..21040
                     /gene="asnB"
                     /locus_tag="JNZ05_001308"
     CDS             19376..21040
                     /gene="asnB"
                     /locus_tag="JNZ05_001308"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HAY8564603.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            21420..21496
                     /locus_tag="JNZ05_001309"
     tRNA            21420..21496
                     /locus_tag="JNZ05_001309"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21454..21456,aa:Met,seq:cat)
     gene            21505..21589
                     /locus_tag="JNZ05_001310"
     tRNA            21505..21589
                     /locus_tag="JNZ05_001310"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21539..21541,aa:Leu,seq:tag)
     gene            21613..21687
                     /locus_tag="JNZ05_001311"
     tRNA            21613..21687
                     /locus_tag="JNZ05_001311"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21645..21647,aa:Gln,seq:ttg)
     gene            21722..21796
                     /locus_tag="JNZ05_001312"
     tRNA            21722..21796
                     /locus_tag="JNZ05_001312"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21754..21756,aa:Gln,seq:ttg)
     gene            21812..21888
                     /locus_tag="JNZ05_001313"
     tRNA            21812..21888
                     /locus_tag="JNZ05_001313"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21846..21848,aa:Met,seq:cat)
     gene            21937..22011
                     /locus_tag="JNZ05_001314"
     tRNA            21937..22011
                     /locus_tag="JNZ05_001314"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21969..21971,aa:Gln,seq:ctg)
     gene            22049..22123
                     /locus_tag="JNZ05_001315"
     tRNA            22049..22123
                     /locus_tag="JNZ05_001315"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22081..22083,aa:Gln,seq:ctg)
     gene            complement(22277..23452)
                     /gene="ubiF"
                     /locus_tag="JNZ05_001316"
     CDS             complement(22277..23452)
                     /gene="ubiF"
                     /locus_tag="JNZ05_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HAY8564604.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGVGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            23598..25022
                     /gene="miaB"
                     /locus_tag="JNZ05_001317"
     CDS             23598..25022
                     /gene="miaB"
                     /locus_tag="JNZ05_001317"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAY8564605.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            25136..26215
                     /locus_tag="JNZ05_001318"
     CDS             25136..26215
                     /locus_tag="JNZ05_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAY8564606.1"
                     /translation="MFSFRRHTDKRNGLNIDTREITLEPADNARLLSLCGPFDDNIKQ
                     LERRLGIEINRRDNHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAI
                     KEARVLEQSAESVPEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTG
                     KTYLAVAAAVDALERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFE
                     MLGFEKVEKLIERNVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNS
                     KAVITGDVTQIDLPRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYE
                     AREEAEQKRKAALAAERKREEQEQK"
     gene            26212..26679
                     /gene="ybeY"
                     /locus_tag="JNZ05_001319"
     CDS             26212..26679
                     /gene="ybeY"
                     /locus_tag="JNZ05_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAY8564607.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            26769..27647
                     /gene="corC"
                     /locus_tag="JNZ05_001320"
     CDS             26769..27647
                     /gene="corC"
                     /locus_tag="JNZ05_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HAY8564608.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFGMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            27672..29210
                     /gene="lnt"
                     /locus_tag="JNZ05_001321"
     CDS             27672..29210
                     /gene="lnt"
                     /locus_tag="JNZ05_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HAY8564609.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            29607..30514
                     /gene="gltI"
                     /locus_tag="JNZ05_001322"
                     /pseudo
     CDS             29607..30514
                     /gene="gltI"
                     /locus_tag="JNZ05_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
     gene            30684..31424
                     /gene="gltJ"
                     /locus_tag="JNZ05_001323"
     CDS             30684..31424
                     /gene="gltJ"
                     /locus_tag="JNZ05_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HAY8564610.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTASRVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLT
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            31424..32098
                     /gene="gltK"
                     /locus_tag="JNZ05_001324"
     CDS             31424..32098
                     /gene="gltK"
                     /locus_tag="JNZ05_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HAY8564611.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            32098..32823
                     /gene="gltL"
                     /locus_tag="JNZ05_001325"
     CDS             32098..32823
                     /gene="gltL"
                     /locus_tag="JNZ05_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HAY8564612.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            32941..33876
                     /gene="rihA"
                     /locus_tag="JNZ05_001326"
     CDS             32941..33876
                     /gene="rihA"
                     /locus_tag="JNZ05_001326"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HAY8564613.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTLGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            33960..35630
                     /locus_tag="JNZ05_001327"
     CDS             33960..35630
                     /locus_tag="JNZ05_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HAY8564614.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDKQRKHTRLAADLAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(35690..37141)
                     /gene="djlC"
                     /locus_tag="JNZ05_001328"
     CDS             complement(35690..37141)
                     /gene="djlC"
                     /locus_tag="JNZ05_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HAY8564615.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIINRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(37138..37845)
                     /locus_tag="JNZ05_001329"
     CDS             complement(37138..37845)
                     /locus_tag="JNZ05_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY8564616.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         9286 a         9718 c         9610 g         9362 t
ORIGIN      
        1 aaatgaagaa cgtgtaccag cagaatatct gccacgtatt tctggtgtct tcgagcattg
       61 cgaatcacgg aaagagtttt atggacgtca gttagaaacc gctgctagcc attatgaaac
      121 gcaactgcgc ccgcccttct tccgcgcact ggtcgattat gtcaatcagg gcaatagcgc
      181 ttttgactgc ccgggtcatt agggaggtga gtttttccgt cgtcatccgg cagggaatca
      241 gttcgtggag tacttcggag aaacgctgtt ccattccgac ttatgcaacg cagacgtagc
      301 aatgggcgat ctgctgattc acgaaggcgc gccatgcatt gcacagcaac atgcggcaaa
      361 agtgtttaat gccgataaaa catacttcgt tctaaatggc acttcatctt ctaacaaagt
      421 ggttttaaac gccctgctaa caccgggtga tctggtgctg tttgatcgca ataaccacaa
      481 atctaaccac cacggagcgt tgctacaggc tggtgcaaca ccggtttatc tggaaacggc
      541 acgtaacccg tatggcttta tcggtggcat tgatgcgcac tgttttgaag aaagttacct
      601 gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa gaggctcgtc ctttccgcct
      661 cgctgtgatt cagttaggca cctacgacgg tacgatttat aacgcccgcc aggtggtgga
      721 taaaattggt catctgtgtg actacattct gtttgactcc gcatgggttg gctatgagca
      781 atttatcccg atgatggctg actgttcgcc gctgttgctg gatcttaatg agaacgatcc
      841 gggtattcta gttacgcaat ccgtacataa acagcaggct ggtttttctc aaacttcaca
      901 aatccataaa aaagacagcc acatcaaagg gcaacagcgt tatgtaccgc acaaacgcat
      961 gaacaacgcc tttatgatgc acgcctccac cagcccgttc tatccgctgt ttgccgcact
     1021 ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt aatatgtgga tggattgtgt
     1081 ggtaaatggc attaatgccc gcaaactgat cctcgataac tgtcagcata ttcgtccgtt
     1141 cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat gaaacagcgc aaattgcggt
     1201 tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg cattcttttg aaggctatgc
     1261 agagaatcaa tactttgtcg atccttacaa actgttgctg acaaccccag gtattgatgc
     1321 acgtaacggc gaatatgaag cgttcggtgt acccgcgacg attcttgcta acttcctgcg
     1381 cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc atcctcttcc tgctgactcc
     1441 ggcagaagat atggccaaac ttcagcaact tgttgccctg ctggtacgct tcgaaaaact
     1501 gcttgagtcc gacgcgccat tagcagaagt gctgccttcc atctacaaac agcatgaaga
     1561 gcgctacgcc ggttataccc tgcgtcagtt gtgccaggaa atgcatgatt tgtatgcccg
     1621 ccacaacgtg aaacaactgc aaaaagagat gttccgcaaa gcgcacttcc cacacgtcag
     1681 catgaatccg caagaagcca actacgccta tttacgcggt gaagtggagc tggttcgtct
     1741 gccggatgcc gaaggtcgta tcgctgccga aggggcgctt ccttatcctc cgggtgtgct
     1801 gtgtgttgtt ccgggtgaaa tctggggtgg tgctgttttg cgttacttca gcgctctgga
     1861 agaagggatc aacctgctgc caggttttgc accggagctg cagggtgtct atatcgaaga
     1921 acatgatggt cgtaagcaag tttggtgcta tgtcatcaag cctcgtgatg cgcaaagcac
     1981 cctgttgaaa ggggaaaaat tatgagtcag gctaaatcga acaaaatggg cgtcgttcag
     2041 ttaaccatac tgacgatggt caacatgatg ggctccggta tcatcatgct gccgacaaag
     2101 cttgccgaag ttgggacaat ttcaattatc tcctggctgg tgacagccgt aggctcaatg
     2161 gcactggcat gggcattcgc aaaatgcggt atgttcagcc gtaaatcagg tggtatgggc
     2221 ggttatgccg agtatgcttt cggtaaatcc ggtaacttta tggcgaacta tacctacggc
     2281 gtctcactgc tgattgctaa cgtcgcgatt gctatttcgg cggttggtta cggcaccgaa
     2341 ctgctcggca cgagtttgtc gccagtgcag attggtcttg cgaccatcgg ggtgcagtgg
     2401 atttgtaccg tggctaactt tggtggtgcg cgcattaccg ggcaaatcag tagcattacc
     2461 gtgtgggggg tcattattcc ggtggtaggc ctgtgcatta tcggctggtt ctggtttagc
     2521 ccgacgcttt acgttgattc ctggaatccg catcatgcac cgttcttcag tgcagtaggt
     2581 tcttccatcg ctatgacgct gtgggctttt cttggtctgg agtctgcgtg tgcgaatact
     2641 gatgtagtgg aaaacccgga acgtaatgtg ccaatcgcgg tactcggcgg tacgttaggt
     2701 gcggcggtga tttatatcgt ctccaccaac gtgattgcag ggattgtgcc taatatggag
     2761 ctggcaaatt caacggcacc atttggcctg gccttcgcac agatgttcac accagaagtg
     2821 ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg gttcgctgct tggctggcag
     2881 ttcaccattg cccaggtgtt taaatcttca tctgatgaag gctacttccc taaaattttc
     2941 tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt tgaccattgt gattatccag
     3001 agcgggttgc cgctgatgac gattagcccg tcgctaaaca gtcagttcaa cgtgctggtt
     3061 aacctggccg tggtgaccaa tatcattccg tatattctgt caatggcggc attagtcatt
     3121 attcagaagg tcgctaatgt gcccccctca aaagcgaaag ttgcaaactt tgttgctttt
     3181 gttggcgcga tgtatagctt ttatgcgctg tactcatccg gggaagaagc catgctgtac
     3241 ggttccatcg tgaccttcct tggttggaca ctgtatggtc tggtatcacc acgctttgaa
     3301 ctgaaaaata aacacggtta aaaaagcaaa gtaaaaaagg gcgatcttgc gaccgccctt
     3361 tttttattaa atgtgtttac gcgtttttca gaacttcgct aacaatctca accgcttctt
     3421 tctcaatctg cttgcgatgt tcttcaccga ggaagctttc gcagtagatc ttatatgcgt
     3481 cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc agtcatcact ttcagaccgc
     3541 caatagaagc accgttgccc ggagcagcag tcaggcgcgc ggtgatcggg tcacctgcca
     3601 gggtgctggc gctcaccatt tccggagaca gcttagacag cgccgctttt tgtgcggaag
     3661 tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa gcgttttgcc agttcgttgt
     3721 agtgttcctg cgggttctta ccggtgacag cggtgatttc cgccgccagc agacacataa
     3781 tgatgccgtc cttgtcggtg gaccacggcg tgccgtcgaa acgcaggaag gaagcccctg
     3841 cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag accatcaaca aaccatttga
     3901 aacctaccgg tacttctacc agcttacggc ccaagtcatt gaccacacgg tcgatcatcg
     3961 cagaagaaac cagcgtttta ccgacagcaa catctttgcc ccactgcgga cgatgctgga
     4021 acaggtagtt gatcgccacc gccaggtagt ggttcggatt catcaaacct gccggagtga
     4081 cgataccgtg acggtcataa tccgggtcgt tagcaaacgc cagatcgaac ttatcacgca
     4141 gtgccagcag acccgccatc gcacactcgg aggagcagtc catacggatc gcaccgtctt
     4201 tatcgaggtg cataaagcgg aaggtttgat cgacctgatc gttaacgatg gtcaggttga
     4261 ggttgtaata ctcgccaatg cgcttccagt attcgatacc ggaaccgccc aacggatcaa
     4321 cgcccagcgt caggcccgct ttctgaatcg ccgccatatc aacgatatcg gccagccctt
     4381 ccacgaacgg ctgcaccaga tcctgctctt tcacatagcc ggatgccatc gcttcgtcga
     4441 gggagatacg cttcacgcct ttcaggccat cggccatcag tgcgttggcc ctgtcttcca
     4501 ccactttggt gacgttagta tcagccgggc caccatttgg cggattgtac ttgataccac
     4561 catcttccgg cgggttatgg gacggtgtaa tcacgatacc gtctgccagc gggccacctt
     4621 ttttattgtg aaccaggatg gcattggaaa cggcaggcgt cggagtgaag ccattgtttt
     4681 cttgcacaat gacatcaacg ccgttcgctg ccagcacttc cagtacggaa atgaacgcag
     4741 gctcggacag ggcgtgagta tctttaccca catagcaagg gccagtgatg ccgtttttcg
     4801 cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg cggctcgtta aagctgtggc
     4861 gcgctgcact gccacggtga ccggaagtac cgaatttcac cgcgtgctcc gcattccctg
     4921 cttctggttt cagtacataa tattgcgccg tcagttgggc gacgttaatc aaatcactct
     4981 gttgtgcagg ttggcctgca cgattgtgga ttgccattgc tttgtccttt gtctgcaacg
     5041 ttttagatag ttccgcaaac cttctcaatc aattccgccg ggaattgcat cgactgcatg
     5101 atgtgttcga tcatgctgca tttacggccg gtgttggtgt tggtgatcac ccaatacggc
     5161 gtgcctggca catgtttcgg cttggtctga ttaccatttt tcagcagcgt ttgttcatct
     5221 gccgcaaagt acacgcgtgt acgaccgtgc aacgattccg ttgcttcggc aaacgcctgg
     5281 gcgtcaagag aatatagtgt agacaacagc agcataaagc gattgaccgc tcgcttttgc
     5341 tctgcgtatt cgtccgaaag cagaagttca cgcattgcgc gaaccttgtc tttaatcgtt
     5401 ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc gaacctcttt cgtcaccgga
     5461 gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc gtaaaatgtc ggatgcgctc
     5521 tcgccgatat gcttagtgtg gctggcaata tagctgtaga gttcatcatc aacttcaatc
     5581 gttttcatct taatccagtg cagtgtctta gctgaataca agtcgccaag agtatatgaa
     5641 taaatcccgg cagcggatag cgtcaagcct ggcttgcaga aaaactcgga ataaacctta
     5701 tttgttgcag ccctgtggca caatggtcaa cataataccc taacccgaca acgcgaaaaa
     5761 aagaactttg ccatgaaatt aaatatccgc gcgcaaactg cacaaaacca gcacaataat
     5821 tctcccatcg ttcttgtcca tggtctgttt ggtagcctcg acaaccttgg cgtactggct
     5881 cgcgatctgg taaacgatca caatatcatc caggttgata tgcgtaacca cggtctttca
     5941 ccaagagagc cggtaatgaa ttacccggcg atggcgcagg atcttgttga taccctggat
     6001 gcactgcaga tcgacaaagc aacatttatc ggtcactcca tgggcggtaa agcggtaatg
     6061 gcacttactg cactggcccc cgatcgcatc gataaactgg tggcgatcga tatcgcaccg
     6121 gtcgactatc acgtacgccg tcatgatgag attttcgcag ctatcaacgc ggtcagtgaa
     6181 tcggacgcac aaactcgcca gcaagcagca gcaataatgc gccagcatct taatgaagaa
     6241 ggggtgattc agtttctgtt gaaatctttt gttgacgggg agtggcgctt taacgtaccg
     6301 gtattgtggg atcagtatcc gcatattgtg ggttgggaga aaatcccggc atgggatcac
     6361 cctgccctgt ttatccctgg cggcaattct ccgtatgtta gcgagcagta ccgtgatgat
     6421 ttactggcac aatttccaca ggcacgggcg catgtgattg caggcgcggg tcactgggtc
     6481 catgctgaaa aaccggatgc ggtattacgc gctatccgtc gctatctcaa tgattaacgc
     6541 aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg tgggcgttgg cgtgactcat
     6601 cgcgctgatg tattatggcg cgctatccat ccgggcagag gcctggctga ttgtttcccc
     6661 cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt acgacattag atctgttcgc
     6721 gcacgagcgt cgaccgggac gaccgaaaac taatccgctt tcgcgcgatg aacagctgcg
     6781 tattaataaa cgcaaccagc taaaacgcga caaagtacgt ggccttaagc gtgtcgaact
     6841 gaagctgaac gcggaagctg tcgaggcgct gaacgagctg gcggaatcgc gtaatatgag
     6901 ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg gcggcgctgc gtagtcaggg
     6961 catcgtttaa atttccactt tcatgtagca cagtgtgcag tcctgctcgt ttgctgattc
     7021 cgcaagactg cctgttctgc tatgattgcc tttatccgtg ggcaattttc cacccccatt
     7081 tcaataagtt tcaagaggtt atttcactca tggctatcac tggcatcttt ttcggcagcg
     7141 acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa acagcttggt aaagacgttg
     7201 ccgatgtcca tgacattgca aaaagcagca aagaagatct ggaagcttac gacattctgc
     7261 tgctgggcat cccaacctgg tattacggcg aagcgcagtg tgactgggat gacttcttcc
     7321 cgactctcga agagattgat ttcaatggta aactggttgc gctgtttggt tgtggtgacc
     7381 aggaagatta cgccgaatat ttctgcgacg cattgggcac catccgcgac atcattgaac
     7441 cgcgcggtgc aaccatcgtt ggtcactggc caactgcggg ctatcatttc gaagcatcaa
     7501 aaggtctggc agatgacgac cactttgtcg gtctggctat cgacgaagac cgtcagccgg
     7561 aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat ttctgaagag ttgcatctcg
     7621 acgaaattct taatgcctga tgtgatgcgg cgtagactca tgtctacgcc gtattaatag
     7681 ataatgccaa tcaaaataat tgctacaaat ttgtaacttt tgctgttgta cctgtacaat
     7741 gtcccagtgt tcaagtggcc ttgccgttgt aaatgtaagc tgtgccacgt ttttattaac
     7801 aatatttgcc agggacttgt ggttttcatt taggcgtggc aattctataa tgatacgcat
     7861 tatctcaaga gcaaattctg tcacttcttc taatgaagtg aaccgcttag taacaggaca
     7921 gattccgcat gactgataac aataccgccc taaagaaagc tggcctgaaa gtaacgcttc
     7981 ctcgtttaaa aatcctggaa gttcttcagg agccggacaa ccatcacgtc agtgcggaag
     8041 atttatacaa acgtctgatc gatatgggtg aagaaattgg tctggctacg gtatatcgcg
     8101 tactgaacca gtttgacgac gctggtatcg tcacccgcca caattttgaa ggcggtaaat
     8161 ccgtatttga actgacacag caacatcacc acgatcacct gatttgcctc gactgcggca
     8221 aggttatcga atttagtgat gattccatcg aagcgcgtca gcgtgaaatt gccgcaaaac
     8281 atggcattcg cctgactaac cacagtctct atctttacgg tcactgtgcc gaaggcgatt
     8341 gccgcgaaga tgagcacgcg cacgaaggca aataagccag cctgaacgag aaaagccaac
     8401 ctgcgggttg gcttttttat acaagggaaa taagaaaagc gagagttaca gctctcactt
     8461 atttgttatt actgcgtatt tcctgccaga ccttatcaca atcggctttc accgcctgat
     8521 catttccggt ttgcgttgca cgcagacact gctgataatc cagtacgcat acactttcct
     8581 ggtcggcaaa ctgctgatgt tgtttctctt tcttcagcac gtttaacacg ctctgacagg
     8641 cttcaatttt ttctggcgaa ccttgcgcgg tgttgataca ggcgctatat gcctctttca
     8701 gacggctgtc ttcctgtggt gcggttgatt gcgcacaagc taccagcccc gatgccatca
     8761 tggcgatgag aatcagtttt ttcattgaat gcggcaggca caaaacctgc cgcgtcgggc
     8821 atcagaagat ggtgaatggt gcgattacca taaatttcac gtcacgctca tcctggaaga
     8881 tgttaccgta accgccgccc cagcttggga tgtcggagtg gttgtcgtat tcggtgaagt
     8941 gcagtttgaa catcgtgcct ttggcgcgac cgtcctgaat ggtgtagacc gcatccaggc
     9001 tgtaggcaga ctcttcaata gtccggtttt tgtcgttgta cgcatccgga ttgctctgcc
     9061 aggtcgcagg tttagcatcc catgcgtaaa cgtaggaagc gccgatggcg aagcctggaa
     9121 gattccagtt tttcaggtca tacatcgcac cgaagaagac cgctttttcg ccgttagcgt
     9181 tgaagtagga acggttatcc caccagatat ccaggcggcc gttggaggaa gcgtaggttg
     9241 gggtcatacg ttgcaggaag tatccctgct gactgtcagc cttaacccag gtgccttcga
     9301 ggcgcaaatc cactacgtca gccgcccggt aaccaaaggt caacgcttgc agccaagcgg
     9361 tgccgtcata aagatcgttg acgctgcgat cgtcaacttt atcgcgggta ccatagaact
     9421 ggtagctggt ggttaacggg ctaccggcga tatcaaattt gtagctggct ttggcaaaat
     9481 attgatcgat atacccttcc gcctgaccaa atgccgcttc cagtacgaag ttatttttga
     9541 agtcgtattt cgcaccgatg gagtgcagat aatcaacttt ggtggtttta tcgttctgat
     9601 aaaactcatc catttccaga tgccacggcg ctttgtattc gttggtccac atgtaggaga
     9661 aactcaaggc accagcatcg ccgtaatcaa aattcgcccc ggcttccgcc ccctgataag
     9721 tacccggcat aaagctccag tgcggcgcta acagcgtttg accggttggc tgaatgtaac
     9781 ctgccctcgc ccaaaccgga ccgtatttaa atttggccgc agctttatac aggcttatac
     9841 cgcttttgtc gccggaccag tcttcgtcat aggctttatt actttttgaa aacgcgattt
     9901 cgttcgggtg ggagctgtcg ccgttttccg ccatttcaat cgccgtaaac gcagcaatat
     9961 caaggccgaa catatcagca gcataaccgg actgaaaatc gaggttggca ttccaggtgg
    10021 agtgagaaag gttggttttg tatttgtcgc cgtcggtaac atctttacgg tcgcgttcac
    10081 gctgccagta atagataccg ccggttaagg ttgaatcgtc gatgaatcct tcggcgcgag
    10141 cctccggcat tgccataaag cccgacattg ctgtaacacc ggcaatagcc agcgccagcg
    10201 tactacgttt gccactaaac gtacgcatgg gttaatcctc tttgacgtat aaattgctgc
    10261 accaaaggtg aacagcgaaa aaaataaaaa ttaaaaaagt agcgttgaga aaagtctcgc
    10321 tgactacagg aacagactat ttttcgcgac gcgaattatc aatctctttc tgtaaccaga
    10381 atgtaagaac atgagcaacc gcacatattt tgatgatttt tgttacaagg cttaagtttg
    10441 cggttgaaag gcttgcagaa gaataaaaac gtgaaattcc agcaagatga aagcggttac
    10501 attttattta attatgtttt gtgattttaa atttatttta tggataaatt gcgaagcgga
    10561 attaattcgc gaagaaaagc agaaaaaacg ccgcagagcg cggcgtttgg gattgtttgc
    10621 tatttcttgc cggatgcggc gtgaacgcct tatccggcct acaaaagcac gcaaattcaa
    10681 tatattgcag agatcatgta ggcctgataa gcgaagcgca tcaggcaatt tagcgtttga
    10741 aatttattgt ctatttattg ccggatgcga cgtaaatgcc tcacccggct tggtagaact
    10801 taaaattact cgcctacttt cgcccaggta tcacgcagcc caacggtgcg gttaaatacc
    10861 ggtttttcgg ccgtggaatg gcggctgtcg aggcagaagt aaccttcacg ctcaaactgg
    10921 aatgctttac ccgctaccgc atctttcagc gacggttcag caaagccctg tttgatcacc
    10981 agcgattccg ggttaatcac cgacaggaaa tcatccgcag cacctgggtt aggcacgctg
    11041 aacagacgat catacaaacg gatttcaacc ggcagcgcat gtgccgcgct cacccagtga
    11101 ataacacctt tgactttacg accatctgcc ggatctttgc ttaaggtgtc ggcgtcataa
    11161 gtacagaaga tggtggtgat attaccttcg gcatctttct ccacgcgttc agccttaatg
    11221 acgtaagcat tacgcagacg cacttcttta cccagcacca gacgtttgta ctgcttgtta
    11281 gcttcttcgc ggaaatcggc gcgatcgatc caaatttcac cgctaaacgg cacctgacgg
    11341 ctccctattt ccggtttgtt cggatggttc ggcatggtga ccatttcgcc ttcgccctga
    11401 tagttttcga taaccagttt caccggatcg ataactgcca ttgcgcgcgg cgcattttcg
    11461 ttgagatctt cacggatgca ggattccagc gacgccatct caatggtgtt gtcctgcttg
    11521 gtcacgccga tgcgtttgca gaactcacga atagaagccg cagtgtaacc acgacgacgc
    11581 agaccggaaa tggtcggcat acgcgggtca tcccagcctt caacgtgctt gtcggtcacc
    11641 agcaggttca gcttacgctt ggacatcaca gtgtattcca gattcaggcg cgagaattcg
    11701 tactggcgcg gatgaacagg aatggtgatg ttatccagta cccagtcgta cagacgacgg
    11761 ttgtcctgga actcaagcgt acacagagag tgcgtaatac cttccagcgc atcgctgatg
    11821 caatgggtga agtcgtacat cgggtagatg caccacttgt tgccagtctg gtggtgttca
    11881 gcaaatttaa tgcggtacag caccggatcg cgcattacga taaatggcga cgccatgtcg
    11941 attttcgcac gcaggcaggc tttaccttct tcaaaaccac cggcacgcat tttttcgaac
    12001 agcgccaagt tctcttcaac gctgcggtcg cggtacgggc tgtttttacc cggctgcgtc
    12061 agggtaccgc ggtattcgcg gatctgttcc ggtgtcagtt catcaacgta cgccaggcct
    12121 ttattgatca gttcgatcgc ataggcgtgg agctgatcaa aataatcgga ggagtaacgg
    12181 acgttaccag accagtgaaa acctaaccac tctacgtcgt ttttgatcga atcgacatac
    12241 tcgatatctt cttttaccgg gttagtgtcg tcgaaacgca ggttgcactg gcctttatag
    12301 tcctgggcga tcccgaagtt caggcagata gatttcgcat ggccaatatg cagatagcca
    12361 ttcggctccg gcgggaaacg ggtgtgtact gtggtgtgct taccactggc cagatcttca
    12421 tcgatgatct gacggataaa gttagtcggg cgggcttctg cctcactcat cgtggattcc
    12481 tcaaagcgta aacaacgtat aacggcgtat gatcttataa gcgggacagg ctgacaactg
    12541 ttagtttttt aaaattgata atctaacaag gattatgggg atgattgatg aaaaaaaaag
    12601 cggcagagaa gatctccacc gcttgtcatt gttggatgcg acgctgccgt gtcgcatcag
    12661 gcatacagca gattactttt tgatttcata cagcggtgtt tgacccgcca caacatggcc
    12721 ctgagcttta atgatcaagc cactgaaatc gtcgatattg ctgcaaacca ccgggctaat
    12781 catcgagcgg gcgttagcgt tcaggtaatc cagatccatt tccagaatcg gttgccctgc
    12841 gcttacctgc gcaccctctt ccaccagacg tttaaagcct ttaccttcca gcgctacggt
    12901 gtcgataccc atatggacga cgatctccgc gcctttttcg gtttccaggc agaacgcgtg
    12961 gttggtgttg aagattttca cgattgtccc tgcggctggt gatacgacga ttttatctgt
    13021 cggtttcacc gccacaccgt cacccaccgc tttgctggcg aatgcttcgt caggaacctg
    13081 atccagtgcc acgacatcac cggtaatcgg cgataccagc tccgcgatag ataccgcgtt
    13141 tggtacagcc tgcggttttg ctacaggcgc ggcagttgcc ggagttgctt cagctgacgc
    13201 agcggctacc ggaccacggg caacgacttt cttcatcgca tcgccgatgg attctgcttt
    13261 cgcgccaaca atcacctgaa tagtctgttt gttcagtttc actacaccag aagcacccag
    13321 acgtttacac atcgtatcgt tgacgcgggc agagtcagcc acggtcaggc gcagacgggt
    13381 aatacaggca tcaatcgctt tcaggttgtc agtaccgcca accgcagcaa tatagttggt
    13441 tgccagttga gtcagacctt cttcagtatt gctgttggct tcttcagtaa cgatctcgtc
    13501 ttctttatct tcacgacccg gcgttttcag gttgaacatg cggataacca aactgaacac
    13561 cacgaagtag atagcgaaga agataacgcc catcaccagc agcatccaga cgttctggct
    13621 ggcggccggc aggttataca tcaacgcgta gtcgatagca cccgcagaga aagagaagcc
    13681 ggcatggata cccagcagcg ttgccacaaa caggctgata ccggtcagca gtgcgtgcag
    13741 gaggtacagc agcggcgcaa ggaacatgaa caggaattcc agcggctcag tcacaccggt
    13801 caggaacgca gtaacagcaa cagaaagcag catcccgcca accatcggac gacgctcttt
    13861 cggtgctgcg aagtacatcg ccagcgccgc acccggcaga ccgaacatca tgatcgggaa
    13921 gaagccggac atgaacatcc ccgcggtgcc gtcaccagcg tagaaacggt tgatgtcgcc
    13981 gtggaaaacc gtacccgccg cgttggtgaa ttcaccaatc tggaaccagg cgatggtgtt
    14041 cagcacctga tgcagaccgg ttgggatcag cagacggttg atgaaaccaa agataccgga
    14101 acccagcgcg cccgcagaaa cgatccactc gccgcctgca tggatagcgt gctgtaccgg
    14161 cggccagacg taaccaaaaa tggccgccag caccagacag aagaagccgg tggcgatcgg
    14221 cacaaagcgt ttgccgccga agaagctcag gaagtccggc agtttaatat cggaccaacg
    14281 gttataggct gcgccaccaa ccagaccggt aatgataccc gccagtacac ccatgttaat
    14341 ttctgggttg atgatcacca tcgctttggt taacacaaag taacctaccg cacccgccag
    14401 cgccgccgca cctgcgctgt ctttcgacca gctggatgcc acaccgatgg cgaagattaa
    14461 tgcgaggtta tcaaaaattg caccgcccgc ctgggcaata aacgcaacgt taagtaaatc
    14521 tggctgaccg aatcgcagca acagcgccgc caccggcagc accgcgatag ggagctgtaa
    14581 cgccctaccg agtcgctgga aaaaacctaa aatattcatc ttattccccc tacgagaacc
    14641 ctatttggct cgtttaaagc cgtattttta ttttgctgca aattttactg ccgatgttct
    14701 gtaatcagat tgttagatca tctgctacag agtgtgtgaa aatttaattc gtatcgcaaa
    14761 ttaaacgcgt gtcttttgtg agttttgtca ccaaatatcg ttatcatcac tcccttttac
    14821 tggctaaacc agaaaactta ttttatcatt caaaaaatca agtcggattg acgcctgtct
    14881 gcgcaaatcc aggttacgct taaagatgtc taatccgcca acggcttaca ttttacttat
    14941 tgaggtgaat aatgagactg atccccctga ctaccgctga acaggtcggc aaatgggctg
    15001 ctcgccatat cgtcaatcgt atcaatgcgt tcaaaccaac tgccgatcgt ccgtttgtac
    15061 tgggcctgcc gactggcggc acgccgatga ccacctataa agcgttagtc gaaatgcata
    15121 aagcaggcca ggtcagcttt aagcacgttg tcaccttcaa catggacgaa tatgtcggtc
    15181 tgccgaaaga gcatccggaa agctactaca gctttatgca ccgtaatttc ttcgatcacg
    15241 ttgatattcc agcagaaaac atcaaccttc tcaacggcaa tgccccggat atcgatgccg
    15301 agtgccgcca gtatgaagaa aaaatccgtt cttacggaaa aattcatctg tttatgggcg
    15361 gtgtaggtaa cgacggtcat attgcattta acgaaccggc gtcttctctg gcttctcgta
    15421 ctcgtatcaa aaccctgact catgacactc gcgtcgtgaa ctctcgtttc tttgataacg
    15481 atgtcaatca ggtgccaaaa tatgccctga ctgtcggtgt tggtacactg ctggatgccg
    15541 aagaagtgat gattctggtg ctgggtagcc agaaagcact ggcgctgcag gccgccgttg
    15601 aaggttgcgt gaaccatatg tggaccatca gctgtctgca actgcatccg aaagcgatca
    15661 tggtgtgcga tgaaccttcc accatggagc tgaaagttaa gactttaaga tatttcaatg
    15721 aattagaagc agaaaatatc aaaggtctgt aattgttatc cctgccctag ttccttgctc
    15781 agggcaatat tttttaaaat cgggggtcag aatgtatgca ttaacccagg gccggatctt
    15841 taccggccac gaatttcttg atgaccacgc ggttgttatc gctgatggcc tgattaaaag
    15901 cgtctgtccg gtagcggaac tgccgccaga gatcgaacaa cgttcactga acggggccat
    15961 tctctccccc ggttttatcg atgtgcagtt aaacggctgc ggcggcgtac agtttaacga
    16021 caccgctgaa gcggtcagcg tggaaacgct ggaaatcatg cagaaagcca atgagaaatc
    16081 aggctgtact aactatctgc cgacgcttat caccaccagc gatgagctga tgaaacaggg
    16141 cgtgcgcgtt atgcgcgagt acctggcaaa acatccgaat caggcgttag gtctgcatct
    16201 ggaaggtccg tggctgaatc tggtaaaaaa aggcacccat aatccgaatt ttgtgcgtaa
    16261 gcctgatgcc gcgctggtcg atttcctgtg tgaaaacgcc gacgtcatta ccaaagtgac
    16321 cctggcaccg gaaatggttc ctgcggaagt catcagcaaa ctggcaaatg ccgggattgt
    16381 ggtttctgcc ggtcactcca acgcgacgtt gaaagaagca aaagccggtt tccgcgcggg
    16441 gattaccttt gccacccatc tgtacaacgc gatgccgtat attaccggtc gtgaaccggg
    16501 cctggcgggc gcgatcctcg acgaagctga catttattgc ggtattatcg ctgatggcct
    16561 gcatgttgat tacgccaaca ttcgtaacgc taaacgcctt aaaggcgaca aactgtgtct
    16621 ggttaccgac gccaccgcgc cagcaggtgc caacattgaa cagttcattt ttgcgggtaa
    16681 aacaatatac taccgtaacg gactttgtgt ggatgagaac ggtacgttaa gcggttcatc
    16741 cttaaccatg attgaaggcg tgcgtaatct ggtcgaacat tgcggtatcg cactggatga
    16801 agtgctgcgt atggcgacgc tctatccggc gcgtgcgatt gacgttgaaa aacgtctcgg
    16861 cacgctcgcc gcaggtaaag tagccaacct gaccgcattc acacctaatt ttaaaatcac
    16921 caagaccatc gttaacggta acgaggtcgt aactcaataa gagaaagtat gacaccaggc
    16981 ggacaagctc agataggtaa tgttgatctc gtaaaacagc ttaacagcgc ggcggtttat
    17041 cgcctgattg accagtacgg gccaatctcg cggattcaga ttgccgagca aagccagctt
    17101 gcccccgcca gcgtaaccaa aattacgcgt cagcttatcg aacgcgggct gatcaaagaa
    17161 gttgatcagc aggcctccac cgggggccgc cgcgctatct ccatcgtcac cgaaacccgc
    17221 aatttccacg caatcggcgt acggcttggt cgtcatgacg ccaccatcac tctgtttgat
    17281 ctcagcagca aagtgctggc agaagaacat tacccgctgc cggaacgtac ccagcagacg
    17341 ctggaacatg ccctgctgaa tgccattgct cagtttattg atagctacca gcgcaagctg
    17401 cgcgagctaa tcgccatttc ggtgatcctg ccagggcttg ttgacccgga cagcggcaaa
    17461 attcattaca tgccgcatat tcaggtagaa aactgggggc tggtagaagc actgaaagaa
    17521 cgttttaaag tgacctgttt cgttggtcac gatatccgta gtctggcgct ggcggagcac
    17581 tacttcggtg caagtcagga ttgcgaagac tccattctgg tgcgtgtcca tcgcggaacc
    17641 ggggccggga ttatctctaa cgggcgcatt tttattggcc gcaacggtaa cgtcggtgaa
    17701 attggccata ttcaggtcga accgctgggt gaacgctgcc actgcggcaa ctttggctgc
    17761 ctggaaacta tcgccgccaa cgctgccatt gaacaacggg tgttgaatct gttaaagcag
    17821 ggctaccaga gccgtgtgcc actggacgac tgcaccatca aaactatctg caaagccgcg
    17881 aacaaaggcg atagcctggc ctcggaagtg attgagtatg tcggtcgtca tctgggtaaa
    17941 accatcgcca ttgctatcaa cttatttaat ccgcaaaaaa ttgttattgc tggtgaaatc
    18001 accgaagccg ataaagtgct gctccctgct attgaaagct gcattaatac ccaagcgctg
    18061 aaggcgtttc gtactaatct gccggtggta cgttctgagc tggatcaccg ctcggcaatc
    18121 ggcgcttttg cgctggtaaa acgcgccatg ctcaacggta ttttgctcca gcatttgctg
    18181 gaaaattaat gtgcttttat agtggcgctt attgttgtca atattctggg tagtccatga
    18241 ccattaaaaa tgtaatttgc gatatcgacg acgtgctgat gcacgataac gtcgccgtac
    18301 cgggtgcagc ggaatttttg cacgggatta tggataaagg cctgccgctg gtgttgctga
    18361 ccaactatcc ttcgcagact gggcaagatc tggcgaaccg ctttgccact gccggtgtcg
    18421 atgtgccgga tagcgtgttt tatacctctg cgatggcgac cgccgatttc ctgcgtcgcc
    18481 aggaaggcaa gaaagcgtat gtggtgggcg aaggcgcact gattcatgaa ctgtacaaag
    18541 ccggtttcac tattaccgat gtgaaccctg atttcgtgat tgttggcgaa acacgttcct
    18601 acaactggga catgatgcat aaagcagcct atttcgtcgc taacggtgca cgctttatcg
    18661 ccaccaaccc ggacacggtt tttatcccgc ttgtggcgcg ttgtgtgcag ggattgagaa
    18721 aatctccggg cgcaaaccgt tctatgttgg taagcccagc ccgtggatca tccgcgcggc
    18781 attaaacaaa atgcaggcgc attcggaaga aacagtgatt gtcggcgata acctgcgtac
    18841 cgatattctg gcgggcttcc aggccggtct ggagacgatt ctggtgcttt ctggtgtttc
    18901 atcgctcgac gatatcgaca gtatgccgtt ccgcccaagc tggatttacc cgtcagtcgc
    18961 tgaaatcgac gttatctgaa taccccccac gtctgcggac gtggttggct ctgatgacaa
    19021 acgccaaact gcctgatgcg ctacgcttat caggcctacg cagctcctgc aatatattga
    19081 attttcatgc ttttgtaggc cgtataaggc gttcacgccg catccggcat gaacaaatcg
    19141 cactttatca gcaatttggc ccccgtaaaa tataaccatt caataaaacc cgctaaaaat
    19201 tatcgattca tcaataaata catcctcaca ccagaccctt tttcaccaat cgacaatcac
    19261 cattacgttt tttatttttg cactgccaaa tagcttgcgc acagtgctgc tttgcggcat
    19321 ttttttaaac aagcaaacac aacaagcaac aaataccagg ttaacggaga aggttatgtg
    19381 ttcaattttt ggcgtattcg atatcaaaac agacgcagtt gagctgcgta agaaagcact
    19441 cgagctgtca cgcctgatgc gtcatcgtgg cccggactgg tccggtattt atgccagcga
    19501 taacgccatt ctcgctcacg aacgtctgtc aattgttgac gttaacgcgg gggcgcaacc
    19561 tctctacaac caacaaaaaa cccacgtact ggcggtaaac ggtgaaatct acaaccacca
    19621 ggcattgcgc gctgaatatg gcgatcgtta tcagttccag acagggtctg actgtgaagt
    19681 gatcctcgcg ctgtatcagg aaaaagggcc ggaatttctc gacgacttgc agggcatgtt
    19741 tgcctttgcc ctgtacgaca gcgaaaaaga tgcctacctg attggtcgcg accatctggg
    19801 gatcatccca ctgtatatgg ggtatgacga acacggtcag ctgtatgtgg cctcagaaat
    19861 gaaatcgctg gtgccagttt gccgcacgat taaagagttc ccggcgggga gctatttgtg
    19921 gagtcaggac ggcgaaatcc gttcttatta tcatcgcgac tggttcgact ttgatgcagt
    19981 gaaagataac gtgaccgaca aaaacgagct gcgtcaggca ctggaagatt ccgttaaaag
    20041 ccatctgatg tctgatgtgc cttacggtgt gctactttct ggtggtctgg attcctcaat
    20101 tatttccgct atcaccaaga aatacgcagc ccgtcgcgtg gaagatcagg aacgctctga
    20161 agcctggtgg ccgcagttac actcctttgc tgtaggtctg ccgggttcac cggatcttaa
    20221 agcagcccag gaagtggcaa accatctggg cacggtgcat cacgaaattc acttcactgt
    20281 acaggaaggt ctggatgcca tccgcgacgt gatttaccac atcgaaactt atgatgtgac
    20341 cactattcgc gcttcaacac cgatgtattt aatgtcgcgt aagatcaagg cgatgggcat
    20401 taaaatggtg ctgtccggtg aaggttctga tgaagtgttt ggcggttatc tttacttcca
    20461 taaagcgccc aacgctaaag aactacatga agagacggtg cgtaaactgc tggccctgca
    20521 tatgtatgac tgcgcgcgcg ccaacaaagc gatgtcagcc tggggcgtgg aagcacgcgt
    20581 tccgttcctc gacaaaaaat tccttgatgt ggcgatgcgc attaacccgc aggataaaat
    20641 gtgcggtaac ggcaaaatgg aaaaacacat cctgcgtgaa tgttttgagt catacctgcc
    20701 cgcaagcgtg gcctggcggc agaaagagca gttctccgat ggcgtcggtt acagttggat
    20761 cgacaccctg aaagaagtgg cggcgcagca ggtttctgat cagcaactgg aaactgcccg
    20821 cttccgcttc ccgtacaaca cgccgacctc aaaagaagcg tatctgtacc gcgagatctt
    20881 tgaagaactg ttcccacttc cgagtgccgc tgagtgcgtg ccgggcggtc cttccgtcgc
    20941 gtgttcttca gctaaagcga tcgaatggga tgaagcgttc aaaaaaatgg acgatccgtc
    21001 tggtcgtgcg gttggcgttc accagtcggc gtataagtaa gaaatgcggt actgccccgg
    21061 agaaatccgg ggctgtttta gttattgcgc tgcttgttga ttctgatatg cagtaaggaa
    21121 atgttccggg tttgccacat cactgtttga caactcatca ctgtaggcca gtatttcata
    21181 gattaatttc ccaccactat tgttgaattc cgaatttcat cctgtacgac aaaagaatgg
    21241 ctgtagggtg gggaaaaaac acaaagtaaa caataattga cgaatatagc gccacgctgt
    21301 tcgcaaccta accaaacagt cactttcgag caattttcct tgaaaaagag gttgacgctg
    21361 caaggctcta tacgcataat gcgccccgca acgccgataa ggtatcgcga aaaaaagatg
    21421 gctacgtagc tcagttggtt agagcacatc actcataatg atggggtcac aggttcgaat
    21481 cccgtcgtag ccaccatctt ttttgcggga gtggcgaaat tggtagacgc accagattta
    21541 ggttctggcg ccgcaaggtg tgcgagttca agtctcgcct cccgcaccat tcaccagaaa
    21601 gcgttgtacg gatggggtat cgccaagcgg taaggcaccg gtttttgata ccggcattcc
    21661 ctggttcgaa tccaggtacc ccagccatct tcttcgagta agcggttcac cgcccggtta
    21721 ttggggtatc gccaagcggt aaggcaccgg tttttgatac cggcattccc tggttcgaat
    21781 ccaggtaccc cagccatcga agaaataatc tggctacgta gctcagttgg ttagagcaca
    21841 tcactcataa tgatggggtc acaggttcga atcccgtcgt agccaccaaa ttctgaatgt
    21901 gtcgaatacg ttcggcaaat tcaaaaacca atttgttggg gtatcgccaa gcggtaaggc
    21961 accggattct gattccggca ttccgaggtt cgaatcctcg taccccagcc aatttattca
    22021 agacgcttac atcgtaagtg cacccagttg gggtatcgcc aagcggtaag gcaccggatt
    22081 ctgattccgg cattccgagg ttcgaatcct cgtaccccag ccacattaaa aaagctcgct
    22141 tcggcgagct ttttgctttt ctgcgttcat tcaatgtcga atgcgatgtt gacacgtctt
    22201 atccttcaat gtcggatgcg acgctgccgc gtcttatccg acctacggtt ggcacgcatc
    22261 cggcaatgtt gtaaggctac aaccctaacg catatttcag cgcctgacgt ttcaacacgc
    22321 cagcacgctc tgccgccatt aacccgagat tacgcacaaa acgcagcggc ggcagattat
    22381 tactgaatcc ggcataaaac agatccatac cactttgcat aatgaagtta tccgccatgc
    22441 gccgcatctg gtaacgcttg aggacaggat aactggccca cgcttcaccg tagctgcggg
    22501 cattcaccag aacatcaatc agggcatcga catcacgata accaagattc accccctgcc
    22561 ccgccagcgg atggatggta tgcgcggcat cgcccaccag cgcaagcccc ggctgcacat
    22621 actgcaacgc atgtcggcgc gtcagcggaa acgcaccagc ggcaagcggt gtaacgtaac
    22681 ccagacgcga cgggaaatgc ttcgcgattt ccgcctggag ttgcgccata ttcatattct
    22741 gcaactggcg aatacgcgcc ggagagtcat accacaccag cgatgcccag ttatcaaaca
    22801 acggcagaaa cgcacgcggt ccgtccgggg taaattgctg ccaggtgctg tcgccgggat
    22861 cgttctcaca ctgtacgcta atcaacatgc acgactgcgc atactgccag gcatgaacgc
    22921 caattcccgc catctgccgc acctgagaat ttgcgccgtc ggcaccaatc accagtttcg
    22981 cgcggatcgt ttcaccgccc ttcagttcca gctcctgaag atcattatct cgatgtaatg
    23041 caatcagcga ggttggcaca cgtaacgtta ctttcggatg cgcttccagc gcctgccaca
    23101 acgcctgttg caggactgtg ttttccacca tgtagccaag caacggcaac tttaattcgg
    23161 ctgcgtcaaa taccacatgt gccgtttccc actcccaggt ttccagtctg cggtaaggat
    23221 ggcaacgcat agcctgtact gcatcccaga cccctacccc tttaagcaat gataccgaag
    23281 ccgcgctgat cgccgagatc cgcacgtccg gttggctgtc agcgacaaac ggtgcaggtt
    23341 ctgcgtgctc gatcaccgtt accgaaaatc cgtgctgtgc cagccccagc gccagtgcgc
    23401 cgccgaccat tcctccgccg acaatggcaa tttccgttgg ttgatttgtc atggtcaatc
    23461 atcctgttag caatatgctt taagtttaca ggatttttac ccccaagggc tgataacgct
    23521 catactggtc acaacggcag caaagcatta cactatgcgc cctgcattcc tggctactat
    23581 ttcgcaagag caagtcgatg accaaaaaac tccatattaa aacctggggc tgtcagatga
    23641 acgagtacga ttcatcgaag atggccgatc tgctggatgc cacccacggc tatcaactga
    23701 ccgacgtggc ggaagaagcg gatgtgctac tgctgaacac ctgctcaatc cgcgagaagg
    23761 ctcaggaaaa agtcttccat cagttgggtc gctggaaact gttaaaagag aagaatccag
    23821 acctgattat cggcgtcggt ggctgcgtgg catcgcaaga aggcgagcac attcgccagc
    23881 gcgcccacta tgtcgatatt atttttgggc cgcaaacgct gcaccgtctg ccggagatga
    23941 tcaactccgt gcgcggcgac cgcagcccgg ttgtagatat cagcttcccg gaaatcgaga
    24001 agtttgaccg tctgccggaa ccgcgcgccg aagggccgac cgcgtttgtc tccatcatgg
    24061 aaggctgcaa taaatattgc acctactgcg tggtgcctta cacccgtggt gaagaggtaa
    24121 gccgtccgtc tgacgatatt ctgtttgaga ttgcccagct tgcggctcag ggcgtgcgtg
    24181 aagtcaacct gctcggtcag aatgtgaatg cctggcgtgg tgagaactac gacggcacca
    24241 ccggatcgtt tgccgatctg ttgcgtctgg ttgctgcgat cgacgggatc gatcgtattc
    24301 gctttaccac cagccatccg atcgaattca ccgacgatat catcgaagtg taccgcgaca
    24361 cgcctgagct ggtgagcttc ctgcatctgc cggtacagag cggttccgat cgcattctga
    24421 acctgatggg acgtacccat acggcgctgg agtacaaagc gatcatccgt aaactgcgtg
    24481 cggcgcgtcc ggatattcag atcagttctg acttcattgt tggcttccct ggcgaaacca
    24541 ccgaagactt cgagaaaacg atgaagctga ttgccgacgt caatttcgac atgagctaca
    24601 gctttatctt ctctgcacgt ccaggaacgc ctgccgccga tatggttgat gatgttccgg
    24661 aagaagagaa gaagcagcgt ctgtatattc tgcaagaacg tatcaatcag caagcgatgg
    24721 catggagccg tcggatgctc ggcaccaccc agcgtattct ggtagaaggg acttcgcgta
    24781 agagcatcat ggagctttcc gggcgtaccg aaaataaccg cgtggtcaac ttcgagggca
    24841 caccggatat gatcggtaaa ttcgtcgatg tagaaattac cgacgtctac ccgaactctc
    24901 tgcgcggtaa agtggtgcga actgaagatg aaatgggtct gcgcatggca gaaacaccgg
    24961 agtcggtgat tgcccgcacc cgcaaagaaa acgaccttgg cgtgggttat tatcagccgt
    25021 aattctcagg cctgccgttc cggtgggcct tgttttttct ctctttatcc ccatctttat
    25081 cgcaatgctt gcgccttttt cctgtggcgt gaataatttc cttataggcc ggtgaatgtt
    25141 cagcttccgg cggcatacag ataagaggaa cggtttgaac atagacactc gcgaaatcac
    25201 cctggagcca gcagacaatg cgcgtctgtt gagcctgtgc ggcccgtttg atgacaacat
    25261 caagcagctc gaacgccgtc tcggcatcga gatcaatcgc cgcgataacc actttaaact
    25321 gaccggccgt ccgatttgcg tcaccgctgc ggcagacatt ctgcgtagcc tgtatgtcga
    25381 tactgccccg atgcgcggtc agattcagga tatcgaaccg gaacagatcc accttgcgat
    25441 taaagaagcg cgggtactgg agcaaagcgc ggagagcgtg ccggagtacg gcaaagcggt
    25501 caatatcaaa actaaacgcg gcgtaattaa gccgcgcacg ccaaaccagg cgcagtacat
    25561 cgccaatatt ctcgaccatg acattacctt cggcgttggc ccggcgggta cgggtaaaac
    25621 ctacctggca gtggctgcgg cagttgatgc cctggagcgt caggaaattc gccgtattct
    25681 gctgactcgt ccggcggtcg aagccggtga gaaactgggc ttcctgcctg gcgatttaag
    25741 ccagaaagta gacccgtatc tgcgcccact gtacgacgcg ctgtttgaaa tgctgggctt
    25801 tgagaaagtc gagaaactga ttgagcgcaa cgttattgaa gtcgcgccgc tggcctatat
    25861 gcgtggtcgt acgctgaacg acgcgtttat cattctcgat gagagccaga acactaccat
    25921 cgaacagatg aagatgttcc tgacccgtat cggttttaac tcaaaagcgg ttatcaccgg
    25981 cgacgtcaca cagatcgacc tgccgcgtaa tactaaatca ggcttacgtc atgccattga
    26041 agtgctggcc gatgtcgaag agatcagctt taacttcttc cacagcgaag acgtggttcg
    26101 tcacccggtg gtggcgcgta tcgttaacgc ttatgaagcc agggaagaag cagaacaaaa
    26161 acgtaaagcg gcgctggcag cagaacgcaa gcgcgaagaa caggaacaaa aatgagtcag
    26221 gtgatcctcg atttacaact ggcatgtgaa gataattccg ggttaccgga agagagccag
    26281 tttcaaacat ggctgaatgc ggtgatcccg cagtttcagg aagagtcgga agtgactatt
    26341 cgcgtggtcg ataccgccga aagccacagt ctgaatctga cctaccgcgg taaggataag
    26401 ccgaccaacg tgctctcctt cccgtttgaa gtgccgcctg gcatggagat gtcgctactg
    26461 ggcgatctgg ttatctgccg tcaggtggtt gagaaggaag ctcaggagca aggcaaacca
    26521 ctggaggcgc actgggcgca tatggtggtg cacggcagtc tgcatttgtt aggttacgat
    26581 cacattgaag atgacgaagc agaagaaatg gaagccctcg aaacagagat tatgcttgct
    26641 ctgggctatg aggatccgta cattgccgag aaagaataat ttgccaggca tttgactggc
    26701 agacgtcccc gccgttggtg attaacgacg gcgttttgat taactaacat gagaacccat
    26761 acgacgccat gagcgacgac aattcacaca gtagtgacac gataagcaac aagaagggat
    26821 ttttctccct gttactcagc caacttttcc acggtgaacc gaaaaaccgt gacgaactgc
    26881 tggcgctgat ccgtgattcc gggcagaacg accttatcga cgaagatacg cgcgatatgc
    26941 tcgaaggggt gatggacatc gcagaccaac gcgttcgcga catcatgatc ccccgctccc
    27001 agatgattac cctgaaacgc aaccagacgc tggacgaatg ccttgatgtc atcatcgagt
    27061 ccgcccactc acgtttcccg gtgattagcg aagacaaaga tcacattgaa gggattctga
    27121 tggcgaaaga cttgctgccg tttatgcgca gcgatgctga agccttcggc atggacaaag
    27181 tgttacgtca ggcggttgtc gttcctgaaa gtaagcgcgt agaccggatg ctgaaagagt
    27241 ttcgctctca gcgttaccac atggcgatcg ttattgacga attcggtggg gtttccggtc
    27301 tggtgaccat tgaagacatc ctggaactga ttgttggtga gattgaagac gagtatgacg
    27361 aagaagatga tatcgacttc cgtcagctga gtcgtcatac ctggaccgtg cgcgcactgg
    27421 cttccattga agacttcaac gaagcgttcg gcacccactt tagcgatgaa gaagtcgaca
    27481 ctatcggtgg tctggtgatg caggcgtttg ggcatcttcc ggcgcgtggc gaaactatcg
    27541 acatcgacgg ttaccagttc aaagtggcga tggccgatag tcggcgtatt attcaggttc
    27601 atgtcaaaat cccggatgac tcaccccagc caaagctgga tgaataaaac cgaaactgga
    27661 tagataacta catggctttt gcctcattaa ttgaacgcca gcgcattcgc ctgctgctgg
    27721 cgttattatt cggtgcctgc ggaacgctgg ccttctctcc ttacgacgtc tggcctgcgg
    27781 cgattatttc gctgatgggg cttcaggcgt tgacctttaa ccgtcgccca ctccagtctg
    27841 ccgctattgg cttttgctgg ggatttggcc tctttggcag cggtattaac tgggtctatg
    27901 tcagcatcgc gacctttggc gggatgcctg gcccggttaa catcttcctg gtggtgctgc
    27961 tggcggcgta tttgtcgctg tataccggac tgtttgctgg cgtgctgtcg cgtctgtggc
    28021 cgaaaaccac ctggctgcgc gtggcgattg ccgcccctgc tctctggcaa gtgaccgagt
    28081 ttctgcgcgg ttgggtgctg actggcttcc cgtggttaca gttcggctat agccagattg
    28141 atggcccgtt aaaagggctg gcaccgctaa tgggcgtgga agccattaac ttcctgctga
    28201 tgatggttag tggcctgctg gcactggcgt tggtcaaacg caactggcgt ccgctggtgg
    28261 tggccgtcgt gctgtttgcc ctacccttcc cgctgcgtta catccaatgg tttaccccgc
    28321 aaccggagaa aaccattcag gtttcgatgg ttcagggcga tattccgcaa tcgctgaaat
    28381 gggacgaagg ccagcttctt aatacgctaa agatttacta caacgcaacg gcaccgctga
    28441 tgggcaaatc atcgttgatt atctggccgg agtcggcgat aaccgatctg gaaattaatc
    28501 agcaaccgtt cctcaaagca ctggacggtg agttgcgtga taaaggtagc tcgctggtaa
    28561 ccgggattgt cgacgcgcgt ctcaataagc agaaccgcta cgatacctac aacaccatca
    28621 tcacgctggg taaaggtgca ccgtacagct acgaatcagc cgatcgctat aacaaaaacc
    28681 atctggtgcc gtttggcgag tttgtcccgc tggagtcgat tctgcgtccg ttagcaccgt
    28741 tctttgatct gccgatgtcg tcgttcagcc gtgggccata tatccagccg ccgctgtcgg
    28801 caaatggtat tgagcttact gcggctattt gctacgagat cattctcggc gagcaagtgc
    28861 gcgataactt ccgcccggat accgactacc tgctgactat ctccaacgat gcgtggtttg
    28921 gtaaatctat tggtccatgg caacacttcc agatggcgcg aatgcgtgcg ctggagctgg
    28981 cgcgcccact gttgcgcagc accaacaacg gcattacggc ggtgattggc ccgcagggtg
    29041 agattcaggc gatgatcccg cagttcaccc gcgaggtgtt aaccactaac gtgacgccga
    29101 ccaccggact cacaccatac gcacgtaccg gcaactggcc gctgtgggtg ctgacggcac
    29161 tgtttggttt tgctgctgtg ttgatgagtc tgcgtcagcg acgtaaataa tcccttctta
    29221 tcgtgccgga atacatccca tatttcggca cgctcaccgg atgcggcatc agtgcattat
    29281 ccggataaca atattctcct atccaacttt ggcacatcta ttgctttgtt atacaaggca
    29341 aagcttgaac cagcatcagt acaacccggt tgcaccacag caatccaccg gtagcaccaa
    29401 aagagtgcat tttaagaatt ttgcttcaga atggtgcggt gcacctcgca aaaacaaaca
    29461 ataaaacatc aatatcttta catttagcca aactaaatgt tacctaacag gcacaacact
    29521 gcacaataaa gttgcagacg ataacaacac aaacactcac aacgggtatc catgcgttct
    29581 taacgcagaa gataaaggag ttggatatgc aattacgtaa acctgccaca gcaatcctcg
    29641 ccctggcgct ttccgcagga ctggcacagg cagatgacgc cgccccggca gcaggcagta
    29701 cgctggacaa aatcgccaaa aacggtgtga ttgtcgtcgg tcaccgtgaa tcttcagtac
    29761 ctttctctta ttacgacaat cagcaaaaag tggtgggtta ctcgcaggat tactccaacg
    29821 ccattgttga agcagtgaaa aagaaactca acaaaccgga cttgcaggta aaactgattc
    29881 cgattacctc acaaaccgta ttccactgct gcaaaacggc actttcgatt ttgaatgtgg
    29941 ttctaccacc aacaacgtcg aacgccaaaa acaggcggct ttctctgaca ctattttcgt
    30001 ggtcggtacg cgcctgttga ccaaaaaggg cggcgatatc aaagattttg ccgacctgaa
    30061 aggcaaagcc gtagtcgtca cttccggcac tacctctgaa gttttgctca acaaactgaa
    30121 tgaagagcaa aaaatgaata tgcgcatcat cagcgccaaa gatcacggtg actctttccg
    30181 caccctggaa agcggtcgtg ccgttgcctt tatgatggat gacgctctgc tggccggtga
    30241 acgtgcgaaa gcgaagaaac cagacaactg ggatatcgtc ggcaagccgc agtctcagga
    30301 agcctacggt tgcatgttgc gtaaagacga tccgcagttc aaaaagctga tggatgacac
    30361 tatcgctcag gtgcagacct ccggtgaagc ggaaaaatgg tttgataaat ggttcaaaaa
    30421 tccaattccg ccgaaaaacc tgaacatgaa tttcgaactg tcagacgaaa tgaaagcact
    30481 gttcaaagaa ccgaatgaca aggcactgaa ctaattacaa gaaccagggg cggaaattcc
    30541 agccctctcg attgttacgt agcacggaca gactatacgc ctgatggtcg ttccccatcg
    30601 ggcctgaaaa ccgcaatacg ctgggtaaca atcttcgagg gtagcagtta acgctgctac
    30661 cctctttttt ctggagtaga tttatgtcta tagactggaa ctggggtatt tttttacaac
    30721 aagccccgtt cggcaatacc acctatcttg gttgggtctg gagtggtttt caggtgacga
    30781 tcgctttgtc gatctgcgcc tggattatcg ctttcctcgt cggttcattt ttcggcattt
    30841 tacgtaccgt tccaaaccgt tttctctccg gtctgggtac gttgtatgtc gaactgttcc
    30901 gcaacgtgcc gttgattgtg caattcttta cctggtatct ggtgatcccg gagctgctgc
    30961 cggagaaaat cggcatgtgg tttaaggccg agctggatcc caatattcag tttttccttt
    31021 catccatgct ctgcctgggg ctgtttaccg cctcccgtgt ttgcgaacag gtacgcgccg
    31081 ctattcagtc gctaccgcgt gggcagaaga atgccgcgct ggcgatgggc ctgacgctac
    31141 cgcagactta tcgatacgta ctgctgccta atgcttatcg cgttatcgtc ccgccgatga
    31201 cttccgagat gatgaacctg gtgaaaaact ccgccatcgc ctcgaccatc ggtctggtgg
    31261 atatggcggc gcaggcgggt aaattgctcg actactcggc tcacgcctgg gagtcgttta
    31321 ccgccatcac gcttacgtat gtgttgatta acgcttttat catgctggtg atgacgctgg
    31381 ttgaacgtaa agttcgcctg cccggcaaca tggggggcaa ataatgtacg agtttgactg
    31441 gagttccatt gttccttccc tgccatatct gctcgacggg ctggtgatca ccctgaaaat
    31501 caccgtcacg gcagtcgtga ttggtatttt gtggggtacc atgctggcgg tgatgcgctt
    31561 gtccagcttc gcgcctgttt cgtggtttgc caaagcctac gttaacgtat tccgctcgat
    31621 tcctttagtc atggttttgc tgtggtttta cttgattgtg ccgggatttc tgcaaaatgt
    31681 gctgggatta tcgccaaaaa atgatattcg tttaatttcg gcgatggtgg cgttttcgat
    31741 gtttgaagcg gcctactatt cagagattat ccgtgccggt attcaaagta tttcccgtgg
    31801 tcagtcgagc gccgcgctgg cgttggggat gactcactgg cagtcgatga aactgattat
    31861 tctgccgcag gctttccgcg cgatggtgcc gctgctgctc actcagggca tcgtactgtt
    31921 ccaggatacc tcgctggtgt atgtgttatc tctggccgat ttcttccgta ccgcctcaac
    31981 cattggtgag cgtgatggta cgcaggtcga gatgatcctg tttgccggat ttgtttattt
    32041 cgttattagc cttagcgcgt cgttgttggt cagctacttg aaaagaagga cagcataatg
    32101 attaccctga aaaatgtttc aaaatggtat ggtcactttc aggtgctgac cgactgctca
    32161 accgaagtga aaaaaggcga agtggtggtg gtttgcggcc cgtctggttc cggcaaatca
    32221 acgctgatta aaaccgtcaa cggcctcgaa ccagtgcagc aaggtgaaat caccgtcgat
    32281 ggtatcgtgg ttaacgacaa gaaaaccgat ctggcaaagc tgcgctcccg cgtcgggatg
    32341 gtattccagc atttcgagct gttccctcat ctgtcgatta tcgaaaacct gaccctggcg
    32401 caggtgaaag tgcttaaacg cgataaagcg cccgctcgtg aaaaagcatt gaaattgctg
    32461 gagcgcgtgg gactttccgc acatgccaat aaatttccgg cgcagctttc cggcggtcag
    32521 cagcagcgtg tggcaatcgc tcgcgcattg tgtatggctc ctattgcgat gctgttcgac
    32581 gaaccgacat cggcgctgga tccggagatg atcaacgaag tgctggacgt gatggtggaa
    32641 ctggcgaacg aaggaatgac catgatggtg gtgacccacg aaatgggctt tgcccgcaaa
    32701 gtggcgaatc gggtgatctt tatggacgag ggtaaaattg tcgaagactc gccgaaagac
    32761 gctttcttcg atgatccgaa atcggaccgc gcaaaagact tcctcgcgaa aatcctgcat
    32821 taatcactct ggcgcgcata cccaattgcg cgccatctca cgctttatcg tttcgcccct
    32881 cgtatctgtt acatcctggc gttacccttg tcgcaaagaa gcacacaaca aggagcaaca
    32941 atggcactgc caattctgtt agattgcgac ccaggtcatg atgacgctat cgcaatagtt
    33001 ctcgccctcg cctcaccaga gcttgatgtc aaagcaatta cgtcttccgc cggaaaccag
    33061 acaccagaaa aaaccttacg caatgttctg cgtatgctga ccttgcttaa tcgcaccgat
    33121 attccggtgg caggcggcgc ggtaaaaccg ttaatgcgtg agttgattat cgcggacaat
    33181 gtgcacggcg aaagcggtct cgacggcccg gcattaccgg aaccgacgtt cgcaccgcaa
    33241 aactgtacgg cggtggagct gatggcgaaa acgctgcgcg aaagtgcgga acctgtcacg
    33301 attgtttcta ccggaccgca aactaacgtt gccttgctgc tcaatagcca tccggaactg
    33361 catagcaaaa ttgcccgtat cgtgattatg ggcggcgcaa tggggcttgg taactggacg
    33421 cctgcggctg aatttaatat ttacgttgac ccggaagcgg cagaaattgt cttccagtca
    33481 gggatcccgg tggtgatggc cggtctggat gttactcata aagcgcaaat ccacgttgaa
    33541 gacaccgagc gtttccgcgc gattggcaac cctgtttcaa ccattgttgc cgaactgctg
    33601 gatttcttcc tcgaatatca taaagacgaa aaatggggct ttgtcggcgc accgctgcat
    33661 gacccatgca ccatcgcctg gctgttgaaa cccgagttgt ttaccactgt tgagcgctgg
    33721 gttggcgtgg aaacacaggg gaaatatacc ctgggtatga cggttgttga ttattattat
    33781 ctgactggca ataaaccgaa tgccactgta attgtcgatg ttgatcgtca gggctttgtt
    33841 gatttactgg ctgatcgtct gaaattttac gcttaacact cgtcattgat taagatcacc
    33901 acgcgttacc tgggtggtct taatctattt atctcttttt ctttcaagat ataaatgcaa
    33961 tggataatgc agaactcgcc ataggtatcg atctcggcac taccaatagt ttaattgccg
    34021 tctggaaaga cggtgccgcg caattaattc caaataagtt cggtgaatat ttaacaccat
    34081 ccataattag catggatgaa aataatcata ttttagtcgg aaaaccggct gtatcacggc
    34141 gtacttcgca tccggataaa acggcagcgt tatttaaacg tgcaatgggc agtaatacca
    34201 actggcggtt aggcagcgac acatttaacg cgccagaact gtcctctttg gtattacgct
    34261 cattaaaaga agatgccgaa gaatttctgc aacgtccgat taaagatgtg gtgatctccg
    34321 ttccggctta tttcagcgat aaacaacgca agcatacccg tttagcagcg gacttagccg
    34381 ggttaaatgc ggtacgctta attaatgaac ccacagcagc tgcgatggcg tatggcctgc
    34441 atacccaaca aaatacccgt tcgctggtgt ttgatctcgg tggcggcacg tttgacgtta
    34501 cggtgcttga gtacgccacg ccggtgattg aagttcacgc ctccgctggc gacaacttcc
    34561 tcggtggcga agattttacc catatgttgg tcgatgacgt tttaaagcgc gcggatgtca
    34621 caaggaccac gcttaacgag agtgaactgg cagccttgta cgcctgtgtg gaagcggcaa
    34681 aatgtagcaa tcaatcgcca atgcacattc gctggcagta ccaggatgaa acgcgggaat
    34741 gcgaatttta cgagaacgaa ctggaagatt tgtggttgcc gctgctcaat cgcttgcgag
    34801 tgccgattga acaggcgttg cgcgatgcgc gtctgaagcc gagtcaaatc gacagtctgg
    34861 tgctggttgg cggcgcgtca caaatgccgc tggtgcagcg aatcgccgtg cgtctgtttg
    34921 gcaaattacc gtatcaaagt tacgatccga gcaccattgt cgcgctgggc gcagcaatcc
    34981 aggccgcctg ccgcttacgc agtgaagata ttgaagaagt gatcctcact gatatttgcc
    35041 cttactcgtt gggcgttgag gttaaccgcc agggcgtttc cggcattttc tcgccgatta
    35101 ttgaacgaaa caccactgtg cccgtgtcgc gtgtagaaac ttattcaacc atgcacccgg
    35161 aacaggattc aattacggtt aacgtctatc agggagaaaa ccacaaagtt aaaaacaaca
    35221 ttctggtgga atccttcgat gtgccgttga agaaaaccgg ggcttatcag tcgattgata
    35281 ttcgctttag ttatgatatc aacgggttgc ttgaagttga cgtgcttctg gaagacggca
    35341 gcgttaagtc cagagtgatt aaccacagcc cggtaacatt gagcgcgcag cagattgaag
    35401 agagtcggac gcggttatcc gcattgaaaa tttatccgcg cgatatgctc atcaatcgca
    35461 cctttaaagc caaactggaa gagttgtggg cgcgggcgct gggtgacgag cgagaagaga
    35521 ttggccgggt gatcaccgat tttgatgcgg cgctgcagtc aaacgatatg gcccgcgtcg
    35581 atgaggtcag gcggcgggcg agcgtttatt tagccattga gacctcataa tcacggctgc
    35641 cggatgcggc gtaaatgctg catccggcat cggcgcaaaa aacacatcgt caactaaaca
    35701 acatcgcgat tttgccgaga gcataaccta taaccagcac cgttaaactg attttgataa
    35761 tgctggtaat gctctgtggt ttttcaccct catcattttt ttcttcatgg ttttcaggtg
    35821 ttggctcatc gacatcttca tcctccggct cgagttttac tttcgtcaac agcatccggt
    35881 tgataatggc ctctaatgga tgagcatctc cgccttccag ccttgtatta agacgggcca
    35941 gcatctcttg tttcggaaac gcttcaagta ctacctgttc cgctacccga tacaaatcca
    36001 gtaacggtga atcttcttct gtctcgctac cgactctcga tttccaccac gcccatgctt
    36061 tgcgtttgaa gaataacgac tcaataaacg attgacctaa cggcgataat tttccgctat
    36121 gtaaaatttc cgcccataac agccgcgtcc gggcgctctc accaggaaca tatttcagcg
    36181 gttgaccttg cgcatctacc atgctccgcg cttcgataac catcactaat aacgcgaaat
    36241 aatccgggca atgacgcttg ctccactgca acaacaaatt atcagcttgt gtagatgggt
    36301 aactttccca gtacgcgccg agatcagcaa gcaggctttc cccctcgccg cagtacaaac
    36361 gttgtgcaca ctggtaatac cgggcatctt cgctttctgg ttcttccgct tgccactgtt
    36421 tggctacagg aatgagttcc accatacctg tttgcaccga gctgtaccag cgcaacagtt
    36481 tgcgatgtaa ctttgcatca tcaggaatga accacggtcc ttccatcgcc aaaaacgcag
    36541 tgacatagtc aggatggtaa cggcagcaac gttccagcgc gaagtaatat tcaacagtct
    36601 ggttctgtaa cgcgacggga tattccagaa ggctaaggaa atcgaaacaa tcaccagcct
    36661 gaatggcctc aagaaaagcc tccctttctt cctcatccat tccttcgctg tcattaaacg
    36721 aatgccagtt caaacgctct gccaacaaag aagcacaatt taatgacaga tatcgcgctt
    36781 ctatagcgat tgcacacagc ggccagcgta attgatcgac atcctccatg ttccaggtat
    36841 ttaattgctg aataaatttc tgccaagcgg agggctgaaa acgatcactt tctgaatcca
    36901 gtaatgtcct gaatgcacgt agtatttcat gttcagcggt ggcatctttt tcttcatcat
    36961 ctgcttcctc gacaggattt accgccagtc gcagcgcctc ttcataggcc tggcgtaatg
    37021 ctttaaaccc ctgcggatcg gtttcgggat gacacaacgg taagcgagca agataagcct
    37081 ggcggataat gtctatttgc gtcgtgcttt caatttcgag gatttgccaa catgttttca
    37141 taggtcgctc acatccttaa gcgttaccgg gcattcaggc tggacagtta atggctgcca
    37201 gggaagcgtg gcatagaaac ggtcgcttcc cgctgccagc tcttcgaaca tccgactccc
    37261 ggcgctatcg tatttgtaca gggttagcgc ctcgcgtagc gaagcgttgt cttcgcactg
    37321 agaagattgc cagtagagac gccctagtga ataggcagaa aaatagtgca tccagctgtg
    37381 atagtaatgg tgagctcgtg catagacgcg cgattgcagc cataaactct cttcttccgt
    37441 cagccagttg ttgagcacac cggcccgtga cagaaacccc attcgaacat aatcccaggc
    37501 tttgattccc ccttctccac agcacatagc ggtttgggca acgaattggg tataaatcaa
    37561 ccctctctcc gagccaccag caattaatgc tttccactct tcaggggaag agcgttgcca
    37621 tgtcagatat aagccagcta aatcattggc atgaccatca tcagtcatct gataaatagt
    37681 ctgaattaaa tcatcacgat tatttattcc ccaggatgat gagagatcga attcttttat
    37741 cccggtatac atttttggcg agtcatgcgc gggtttgcaa ctctgattaa gtatttccat
    37801 gggcgcagaa agcgcaaaaa gaagatattg ttcttcctta ttcatttttt aataatccct
    37861 taagtgcatt aactgactac ttttttatta ttgatgagta taaacttgac gctttcaaaa
    37921 taaaaagaaa atcgaagcat tcacacatga ataaaaaatt aatgtatata ttcgca
//
LOCUS       DADMEQ010000025        37628 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.025, whole genome shotgun sequence.
ACCESSION   DADMEQ010000025 DADMEQ010000000
VERSION     DADMEQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.025"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..174)
                     /locus_tag="JNZ05_001330"
     CDS             complement(<1..174)
                     /locus_tag="JNZ05_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 domain-containing protein"
                     /protein_id="HAY8564617.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQYR"
     gene            complement(258..1142)
                     /gene="yhdJ"
                     /locus_tag="JNZ05_001331"
     CDS             complement(258..1142)
                     /gene="yhdJ"
                     /locus_tag="JNZ05_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY8564618.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     YYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1228..1524)
                     /gene="fis"
                     /locus_tag="JNZ05_001332"
     CDS             complement(1228..1524)
                     /gene="fis"
                     /locus_tag="JNZ05_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HAY8564619.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1550..2515)
                     /gene="dusB"
                     /locus_tag="JNZ05_001333"
     CDS             complement(1550..2515)
                     /gene="dusB"
                     /locus_tag="JNZ05_001333"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAY8564620.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2844..3725)
                     /gene="prmA"
                     /locus_tag="JNZ05_001334"
     CDS             complement(2844..3725)
                     /gene="prmA"
                     /locus_tag="JNZ05_001334"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAY8564621.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKTEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3737..5188)
                     /gene="panF"
                     /locus_tag="JNZ05_001335"
     CDS             complement(3737..5188)
                     /gene="panF"
                     /locus_tag="JNZ05_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HAY8564622.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5178..5420)
                     /locus_tag="JNZ05_001336"
     CDS             complement(5178..5420)
                     /locus_tag="JNZ05_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HAY8564623.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5529..6878)
                     /gene="accC"
                     /locus_tag="JNZ05_001337"
     CDS             complement(5529..6878)
                     /gene="accC"
                     /locus_tag="JNZ05_001337"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAY8564624.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVQSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNTIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6889..7359)
                     /gene="accB"
                     /locus_tag="JNZ05_001338"
                     /gene_synonym="fabE"
     CDS             complement(6889..7359)
                     /gene="accB"
                     /locus_tag="JNZ05_001338"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAY8564625.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8337..9311)
                     /locus_tag="JNZ05_001339"
     CDS             complement(8337..9311)
                     /locus_tag="JNZ05_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY8564626.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPSTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9463..11403
                     /gene="csrD"
                     /locus_tag="JNZ05_001340"
     CDS             9463..11403
                     /gene="csrD"
                     /locus_tag="JNZ05_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HAY8564627.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVIEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11708..12751
                     /locus_tag="JNZ05_001341"
     CDS             11708..12751
                     /locus_tag="JNZ05_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAY8564628.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12817..13920
                     /gene="mreC"
                     /locus_tag="JNZ05_001342"
     CDS             12817..13920
                     /gene="mreC"
                     /locus_tag="JNZ05_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAY8564629.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13920..14408
                     /gene="mreD"
                     /locus_tag="JNZ05_001343"
     CDS             13920..14408
                     /gene="mreD"
                     /locus_tag="JNZ05_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAY8564630.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14417..15010
                     /gene="yhdE"
                     /locus_tag="JNZ05_001344"
     CDS             14417..15010
                     /gene="yhdE"
                     /locus_tag="JNZ05_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HAY8564631.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15000..16469
                     /gene="rng"
                     /locus_tag="JNZ05_001345"
     CDS             15000..16469
                     /gene="rng"
                     /locus_tag="JNZ05_001345"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HAY8564632.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16537..20337
                     /locus_tag="JNZ05_001346"
     CDS             16537..20337
                     /locus_tag="JNZ05_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein"
                     /protein_id="HAY8564633.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QSAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLIEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKAR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20493..21938
                     /gene="tldD"
                     /locus_tag="JNZ05_001347"
     CDS             20493..21938
                     /gene="tldD"
                     /locus_tag="JNZ05_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HAY8564634.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22066..22995)
                     /gene="aaeR"
                     /locus_tag="JNZ05_001348"
     CDS             complement(22066..22995)
                     /gene="aaeR"
                     /locus_tag="JNZ05_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HAY8564635.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23178..23381
                     /locus_tag="JNZ05_001349"
     CDS             23178..23381
                     /locus_tag="JNZ05_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HAY8564636.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23389..24321
                     /gene="aaeA"
                     /locus_tag="JNZ05_001350"
     CDS             23389..24321
                     /gene="aaeA"
                     /locus_tag="JNZ05_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HAY8564637.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24327..26294
                     /gene="aaeB"
                     /locus_tag="JNZ05_001351"
     CDS             24327..26294
                     /gene="aaeB"
                     /locus_tag="JNZ05_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HAY8564638.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26386..26658
                     /locus_tag="JNZ05_001352"
     CDS             26386..26658
                     /locus_tag="JNZ05_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564639.1"
                     /translation="MNIYAFDFNEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="JNZ05_001353"
     CDS             complement(26715..26978)
                     /gene="yhcN"
                     /locus_tag="JNZ05_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HAY8564640.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATPYQITEARSGDTWHATAELYK"
     gene            complement(27343..27813)
                     /gene="argR"
                     /locus_tag="JNZ05_001354"
     CDS             complement(27343..27813)
                     /gene="argR"
                     /locus_tag="JNZ05_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HAY8564641.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28248..29186
                     /gene="mdh"
                     /locus_tag="JNZ05_001355"
     CDS             28248..29186
                     /gene="mdh"
                     /locus_tag="JNZ05_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HAY8564642.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(29248..30315)
                     /gene="degS"
                     /locus_tag="JNZ05_001356"
     CDS             complement(29248..30315)
                     /gene="degS"
                     /locus_tag="JNZ05_001356"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HAY8564643.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30405..31772)
                     /gene="degQ"
                     /locus_tag="JNZ05_001357"
     CDS             complement(30405..31772)
                     /gene="degQ"
                     /locus_tag="JNZ05_001357"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HAY8564644.1"
                     /translation="MKKQTQLLSALALSAGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPSSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(31926..32324)
                     /locus_tag="JNZ05_001358"
     CDS             complement(31926..32324)
                     /locus_tag="JNZ05_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HAY8564645.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            32518..33645
                     /locus_tag="JNZ05_001359"
     CDS             32518..33645
                     /locus_tag="JNZ05_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HAY8564646.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33864..34292
                     /gene="rplM"
                     /locus_tag="JNZ05_001360"
     CDS             33864..34292
                     /gene="rplM"
                     /locus_tag="JNZ05_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAY8564647.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34308..34700
                     /gene="rpsI"
                     /locus_tag="JNZ05_001361"
     CDS             34308..34700
                     /gene="rpsI"
                     /locus_tag="JNZ05_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAY8564648.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35095..35733
                     /gene="sspA"
                     /locus_tag="JNZ05_001362"
     CDS             35095..35733
                     /gene="sspA"
                     /locus_tag="JNZ05_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HAY8564649.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLINTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35739..36236
                     /gene="sspB"
                     /locus_tag="JNZ05_001363"
     CDS             35739..36236
                     /gene="sspB"
                     /locus_tag="JNZ05_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HAY8564650.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36279..37541)
                     /gene="dcuC"
                     /locus_tag="JNZ05_001364"
     CDS             complement(36279..37541)
                     /gene="dcuC"
                     /locus_tag="JNZ05_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY8564651.1"
                     /translation="MMCGVWLGFGGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLS
                     IMAVGGYARYMERIGASRAMVSLLSRPLKLIRSPYIILSATYVIGQIMAQFITSASGL
                     GMLLMVTLFPTLVSLGVSRLSAVAVIATTMSIEWGILETNSIFAAQVAGMKIATYFFH
                     YQLPVASCVIISVAISHFFVQRAFDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLI
                     LMLGSLFLAHVGLMKSELHLVVVMLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGT
                     QFVNVVTLVVAGEIFAKGLTTIGTVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMG
                     SGNAPFMSFASLIPNIAAGLHVPAVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVS
                     PFAVVKRTAIPMAVGFVVNMIATITLFY"
BASE COUNT         9110 a         9413 c         9904 g         9201 t
ORIGIN      
        1 tctgtactga aaattcagtt cgtcacagtt aaccagtttt agtggattaa ctgagttcat
       61 attacggcat ttgtctgttt ctgttgtcag cagttcaatc cactgcatca gtaaagtatc
      121 aaacagaaaa acgaagacag cgaaaacgac gagccaccaa tacttgcgaa tcattacctg
      181 agcctgtgca tgtttaacca agtctggcag gaaatataca cgaaatgaat aatctgaaaa
      241 ccaggcttac acttccttta ctttgcaatg agatcggggt caacttcgct taaccggctt
      301 cgttttgaca ggttgccggt ctttcttttt ttcactttcg ccagttcttc cgcggagtaa
      361 tgcgacgcga catccagccg tcgaagcccc attttgatgt actcgctgtt gatctcaata
      421 ccaatgaatt ttcgtccgct ggcgatggct acggcaccgg tagtaaagct accggcaaac
      481 gggtcgagaa cgatatcgcc tgggttggaa gaggcgagaa taatgcgttt cagtaaggct
      541 ttcggttttt gcgtcgggtg gttctcatat tcatccatca aataacgcac gcgcggaaaa
      601 tcccagacgt tacccggtac tttttgatga ttgtatggct gtggaggatt tttgcgataa
      661 tagatcaacg cgcgctgcga tccggttttg gcttctacca gaatagcatc accgttgaat
      721 gtgtagttct ttgcgtcttt caccatcatc aggatgggtt cgtacatgga gccgtagtgt
      781 tttttcgcct gtactccaga actgtcatat gaccagacga tgcgactttt gatggtaaaa
      841 agcttgcggc actggagatc gataaagggc atgttttccg tactgttcat aatgtacatg
      901 ctgccctgct ttttcagaac gcggtggcac tctgcaatca cttcaaacag ccagtcgata
      961 aacagatctt ctttccaggc ttcgatcaga ccatcaaaat ttttaccgat gttatacggt
     1021 gggtcggcaa agagcagatc gacgctttca gtaggtagct ttttaagttc ggcaagggca
     1081 tcaccgtgaa taatggtctt agcttcatta ccaaaccggg tcggttcaca tcctgttctc
     1141 atggtcactc cctttgtgac acctataaaa aaggcgcttc cctatgccga gtagcgcctt
     1201 tttaatcaag catttagcta acctgaatta gttcatgccg tattttttca attttttacg
     1261 cagcgtacca cggttgatgc ccatcatcag cgcagcacgg gtctggttac cacgggtgta
     1321 ttgcatcacc atgtccaaca ggggctgttc tacttcagcc agtaccagct catagaggtc
     1381 attcacatcc tgaccattca gttgagcaaa atagttcttc agtgcctgtt taaccgagtc
     1441 acgcaggggt ttttgggtta cctgatcctg agagttaacg gtagaaacgg tcagtacgtc
     1501 agaatttacg cgttgttcga acatagttct gtcagctctt tatttctgtt tacgcaaaat
     1561 tttcgaagta tgcctccaac gcctccagct gttcgctggc atcctcaatg gcgttgaatg
     1621 tgcgccgaaa ctggtcattt ggagcgtgtt cctggagata ccaggaaacg tgtttacgtg
     1681 caattcggta cccttttgcc ggaccataaa agtcatgcag ttcccgaacg tgcgcgcaaa
     1741 gcaagcgctt aacctctgcc aaaggcagcg ggggcagcaa ctccccagtg tccagataat
     1801 gctggatttc ccgaaagatc cagggtcttc cctgagctgc gcggcctatc atcagggcat
     1861 ccgcccctgt atagtcgagc acagctctgg ctttaagcgg gtcagtaatg tcgccattcg
     1921 cgataaccgg aatggaaact ttctgcttaa ctgcccgaat actgtcgtac tcagcttctc
     1981 cattgaacaa acaggcgcgt gtacggccat gaatggtcag agcctgaatg ccacagtctt
     2041 cagccagttg ggcaatctct tcgcagttac ggtgttccgg tgcccagccg gtgcgaatct
     2101 tcagggtaac aggaacgtcc actgcattga cgacctcggt aaggatcgat ttaacgacat
     2161 ccgggtactg caagagggct gaacctgcga gcttgcgatt cactttttta gccgggcaac
     2221 ccatattgat atcaataatc tgggcaccgc tttccacgtt aatacgtgct gcatctgcca
     2281 tttctttcgg agcgctacca gcaatttgca cggtgcgaat accgggttca tcaatgtgca
     2341 ccatccgtaa acgagatttg tcgctttccc aaacctgtgg gttagaagac atcatctcgg
     2401 atactgtcaa tccggctccc atctcgtagc acaacgtccg aaaaggtctg tctgtaatgc
     2461 cagccatggg cgctgcgatc aggcgatttc tgagctgata ttgtccgatg cgcatgagtt
     2521 aagaaatgac catactgtga ctgcaaggcg gcgtatatta cgcatttttt gcacgagatg
     2581 aaaggccaaa ctttgaccaa tcctctgaga tggatcaaag aattgcattt aaaatgagcg
     2641 tggtgcgata attactcata aaaatcatca tattagaaaa tagtgactaa aatttacact
     2701 caaagaaatt tgagtaagtt ctcaattttt ctttatgaat gaaaattttg gcacgcaaat
     2761 ttgcgtaaat aatcggcaaa tttacgtgcc ttttgtgagc ttgctcgcac ttcgccccgc
     2821 gtcaccctac ggcgatgcga aggttaattc ttacgaccgg taatacggca ccactcttct
     2881 ttttccacga ccgggtccag tgcgaagcta tcggcataag cttcacaaac gctctctgcc
     2941 tggcttgcca gaataccgga aaggcccagc aaaccgcctg aaaccggcag gacgctgatt
     3001 aacggtgcca gttcacgtaa tgggcctgca aggatgttag cgaccaccac gtcggctttc
     3061 atttcttctg gctgatcttt cggtaagtag agttccagac ggtcagaaac gccattacgt
     3121 tcggcgttat cgcggctggc ctgaatcgcc tgcggatcga tatcaatacc aatggctttt
     3181 gctgcaccca gtttcagcgc cgcgatcgcc agaatgccgg aaccacagcc aaagtcgatg
     3241 actgttttac cggttaaatc gaggctgtcg agccattgca ggcacagaga ggtggttgga
     3301 tgggtacccg taccaaacgc cagccctgga tctaacatca cgttgacggc gttttcgtcc
     3361 ggcacatcac gccagctagg gcagatccac agtcgttcac caaagcgcat cgggtggaaa
     3421 ttatccatcc attcgcgctc ccagtcttta tcttctagtt gttcggtttt atgcgcgaag
     3481 cctgcgccga gcagcggatg gttttccaga atcgccacca cgtcgttcat atcggtttca
     3541 gcgtcgaaca gaccaatcac atcggtgtcg ccccacaggc gcgtttcgcc cggcagcggc
     3601 tcaaatactg gcgtatcgtg ggtatcctga aaagtgatag aaacggcacc cgcttccatc
     3661 agcgcatcgc taagatcttc cgcgttcgcg ccggtggtgt tcagtttcag ttggatccaa
     3721 ggcatggcaa aactctttat ttatcagtag tcaaaacggt agcttgcggg acggatgtac
     3781 cgaaacggtt tccgaccagg aaagccagca aacttagtag taacgagggc acgatagggt
     3841 ggaagcccag gtactgaata ttcagcgtcg cgagtacggc atacagcacg ccgccaacga
     3901 tcatcgcact tagcgcgcct ttggcgttgg cgcgttccca gtaaagaccc agcaccagcg
     3961 gccacaggaa aacggcttcc agcccaccga aggccagcaa attcagccag atgatcattt
     4021 ctggcggctt ccaggcggca agcagcagca acgcgccgag aactaacgta attaccgccg
     4081 acatccgctt cagacgcgtc tcgttttgca tttgatccgg acggatattc agatagagat
     4141 ctttaatgat cgtagcggaa ctttgcagca gttgggcgtt aattgtcgac atgatcgcag
     4201 ccataggtgc agccagaaag atcccggcag caaaaggtgg cagcactttt accattaacg
     4261 tcgggatcac cagatccggt acggtgagat cggggatcac tgcacgacct aacgctccgg
     4321 ctaggtgcat accgaacatc aggattgcga ctacaatcgt accgatgatg atcccccggt
     4381 gtactgcttt gctgtcttta taagagatac agcgcaccgc agtatgcggc aggccaatca
     4441 cgccaaagca caccagtacc cagaacgacg tcataaaggc aggcgacaga atatcgtcag
     4501 cgccttgtgg cgtaaccagt tgcggatcga tggtttgcaa ggtctgtact gcgttgctta
     4561 agccgccagc ggcatgtacc acgccaataa gcagcacaac ggtgccaatc agcatcacaa
     4621 gcccttgcat ggtgtcgttc agcacgctgg cgcgaaagcc accaaaggcg gtatataacg
     4681 caatgctgat accaaaaatc agcagcccgg tttcataagg aatacccgcc gcggtttcca
     4741 gcaggcgcgc accgccgata aactgcacgg tcatcgcacc aacgaacgca accagcaaac
     4801 tcaaactcgc cagccacacc agaagacgac tctggtagcg ggcaaacagc atatcgttca
     4861 gcgtcactgc attgtagcgg cgcgcaagaa tcgcaaactt cttgccgaga ataccgagtg
     4921 aaagccagac agcaggaagc tgaatcatcg ccagcaatac ccagcccagc ccgtatttat
     4981 aagcagctcc tggcccgccg ataaacgaac tggcactgat ataggtcgcg gtgagcgtca
     5041 tcgccagcac aataccgccc atagagcggc tgccgaggaa atactcatta aggaaggtgc
     5101 cggtgctccg tttacgcatc gcataaaccg agataccgaa caccaccacc agataggcga
     5161 ccagcggtag aattacttca agctgcatcg tcatcctcca gtgggatatc gcgatagata
     5221 aatttcacca tcgcccagca cagtccaata aacagtagcg gcgtcaggat gcaggccatc
     5281 tcaaaccagc gcggaaagcc ggtaaaaccg ggggcaacgc caggtaagta agcatctact
     5341 aaccaaactg ccagatacaa aagggtcagc cccagcgccc agcgcgcctc tttatgggcc
     5401 tgaacaaaac gagtgtccat tttttgtccc tgatgggtga agaaagcgag gattgtacct
     5461 tatgggggtt gtcgatcccc agtaataaaa aaggccggaa aatccggcct tttgacgctt
     5521 ttgcagtctt atttttcctg aagaccgagt tttttctcca gatagtggat gttagtgcca
     5581 ccatgctgga agttctcgtc attcatgatg cggatctgca gatcaacgtt ggttttgata
     5641 ccgtcgatga tcagctcctg cagcgcattc ttcatgcggg caatcgccac gtcacggttt
     5701 tcaccgtagc aaatcagctt accgatcatt gagtcatagt acggcggtac ggtgtagccc
     5761 gcgtagatat gagactccca acgtacgcca aaaccgccag gtgcgtggaa acgggtgatt
     5821 ttgcctgggc ttggcaggaa ggtgttcgga tcttcggcgt tgatacgaca ttccaccgca
     5881 tggccgcgaa cgtgaacttc ttcttgcttg atcgacagcg gttgaccggc agcgatacgc
     5941 agctgttctt tgatcaggtc aacgccggtg atcatttctg taaccgggtg ttctacctga
     6001 atacgggtgt tcatttcgat gaaatagaac tcgccgtttt cgaacaggaa ctcgaaagta
     6061 cctgcaccgc gatagccgat atcaacacac gctttagcgc aacgttcgcc gatgtagcga
     6121 cgcagttccg gggtaatgcc cggtgctggt gcttcttcga ccactttctg gtggcggcgc
     6181 tgcatggagc agtcacgttc cgccagatag atagtgttgc cctgaccgtc agccagtacc
     6241 tgaatctcga cgtggcgagg attctccagg tatttctcca tgtaaaccat atcgttgctg
     6301 aaagcagctt tcgcttccgc acgggtcatg gagatggatt gtgccagttc agcgtcgccg
     6361 cgcactacgc gcataccgcg accgccgccg ccgccggagg ctttgataat caccggataa
     6421 ccaatgcgtt tagcaatggc gcggttttta tccatatcgt cgcccagcgg gccgtcagaa
     6481 ccaggtacgc aaggaacgcc cgcttttttc atcgccgcga ttgcggatac tttgtcgccc
     6541 atcaggcgaa tggtttctgc tttcgggccg atgaagataa agccggagcg ttcaacctgc
     6601 tcggcaaagt tggcgttctc ggagaggaag ccgtaacccg gatggattgc tactgcgccg
     6661 gtgatttcag cggcgctgat gattgccggg atgttcagat aactttgtac tgacggagca
     6721 gggccaatac agaccgtttc atctgccagt aatacgtgtt ttagatcgcg atccgcgctg
     6781 gagtgcacag cgacagtctt gatgcccagt tctttacagg cacgaagaat acgcaatgca
     6841 atctcgccgc ggttggcaat aacaatttta tccagcatgt tcgcctcgtt actcgatgac
     6901 gaccagcggc tcgtcaaatt ctaccggttg tccactttcg accagaattg ctttcacggt
     6961 accggatttg tccgcttcga tctggttcat cattttcatg gcttcaacga tgcacagggt
     7021 atcgcccacg ttgactttct gacccacttc gatgaacgct tttgcgtccg ggcttggggt
     7081 gcggtagaaa gtaccaacca tcggggaacg tacgatgtga ccactgattt ccgctgctgc
     7141 tggcgcttcc atggaaggaa cggtcgccgg agcggctgcg ttagattgag ctggctgctg
     7201 catcattggt gcagcgtaag cttgttgcat cacagggaaa cttgcggcag gagctgcacg
     7261 gctaatgcgt actgactctt cgccttcaga aatttccagt tcggagatgc ctgattcttc
     7321 aaccagctcg atcagttttt taatcttacg aatatccatg agtgggttcc gtactctttg
     7381 tttagtgtga ttgtgacagg cgtttcaccg ccgtctgtaa agcgtatgcc ccgacagctg
     7441 tatgcatagc gataaattcc agcaggccgg gacgccggtc tattttgccc cagagtgtca
     7501 atgttagact tgacggacat tgtgcagcga tctgctgcta tcctccggca aaaaacaaaa
     7561 tataccttcg ttgcgccaat gtcacctttt ccgcagcgca aaaactgcgt cagggaggac
     7621 gaggtcgcac attataacga tttcgtagca attggcagct aaatactggt cttatcaggg
     7681 aagataatca acagctaaca tgtaaataac cttcaacacc gtgtaatttg caacaagccg
     7741 cacaattcac gaaattagcg ccatcatcga cggaacttct tataacgtaa ggtgaaaagc
     7801 tgaaaagcca gccctgcgaa gatgaggggc tgcaatgaga taatcttcac agaccacaaa
     7861 taatgtaggg atgccaggat cgcgacaaga tagacgacat tacgcagcgg ttgctgggga
     7921 ttacccaatt ttcactgcat cgtctggata gatgtgaatg ctaaagcacg caaaatcaac
     7981 caactgacaa ttctcagcat taaataggtt cgagtggtta atttgtagtc aaataaaatc
     8041 agattattca ccccatggta ccagaaatca gtaacaggtt aagcgtagtg tcgcccaggc
     8101 aaagcaccat aaacctaaca ggcgacggag gcgtatcaat aatggctgtt tagaggagta
     8161 tcaatgcgca tcaatttgtg gtcagtcgaa atcatgactt tccagtttgg atgatgttgt
     8221 acaacttcct gaccaaggtg ttcgacgaaa gctgtccggc taaccgcttt tcccgttaac
     8281 cgattgaata atgatgtcaa aatgagttcc ctctcttatt attccttgct aaatggttag
     8341 ttaaccttca ccagcgtgcg accctggatc tggttattaa tgatggcctc ggcaaagttc
     8401 ggtgcctctg acagagatat ctcttttgcc gcctgggtat agaatgattc cggtaaatcg
     8461 gcgaccagtc gctgccaggc ttgtgcgcgg cgttctggtg gcgtcattac tgaatccacc
     8521 ccttgcaaac ggacattacg cagaataaat ggcatgaccg tgcttggcag agtaaaacca
     8581 cccgccagac cacaggccgc cacgcagccg ccgtaattca tttgcgccag cacttttgcc
     8641 agcactttgt cgccaacagt gtcaattgcc ccagcccaga cttgtttttc cagaggacgg
     8701 gattcggcaa actcatcacg agggagaata cggctggcac ctaaactttt cagatattca
     8761 tgggtacttt cgcgaccgga aacggcaacg acctgataac ccaacttatg cagcagcgcc
     8821 acggcggtac tgccgacgcc accactggca cccgtcacga caatctcccc gtcctgcggg
     8881 cgaacaccgg catcttccag cgccatcaca cacagcatgg cggtaaaacc ggcagtaccg
     8941 ataatcattg ctttacgcgc gtccagccct tgcggcatgg caaccagcca gtcacctttc
     9001 actcgcgcct gctccgccag cccaccccag tggttttcac caacgcccca gccagtgagt
     9061 aacacctcct gaccggcatg aaaacgcgga tcttcgctgg tgcgtacagt tccggcaaaa
     9121 tcgatcccag gaatcatcgg aaaattacgg atgatttttc ccttaccggt aatcgccagc
     9181 gcatctttat agttcaggct cgaccagtga acatcgaccg tgacatcgcc ctccggcagg
     9241 cgactttcgt ccagagtctg tactgatgcg agagttttgc cgtcctgctg ttctaaaagt
     9301 aacgcctgca taagtggtcc tcatgtgcat gatggattgg aaaataattt ctgaagacta
     9361 tactcgctaa tggaaaagca aagccgatga agcgcaataa attgcgagat aaatctgatt
     9421 tgctagtatg cccgcttcct cactatcgga gttaacacaa ggatgagatt aacgacgaaa
     9481 ttttcggcct ttgttacgct gctcaccggg ttaacaattt ttgtgacttt gctgggctgt
     9541 tcgctaagtt tctacaacgc cattcagtat aagtttagtc atcgcgttca ggcggtggcg
     9601 acggcgattg atacccacct tgtgtcgaat gacttcagcg cattaaggcc acaaattacc
     9661 gaattaatgg tgtcagcaga tatcgttcgt gtagacctgc tgcatggtga taaacaggtt
     9721 tataccctgg ccagaaatgg tagttatcgt ccggttggca ccagcgatct gtttcgtgaa
     9781 ctgagcgttc cgttgataaa gcatccgggg atgtcgttgc gtctggttta tcaggatccg
     9841 atgggcaact atttccattc gttgatgacc accgcgccgc tcacgggggc gattggcttt
     9901 atcattctta tgctctttct ggcggtacgc tggttacaac ggcaacttgc cgggcaagaa
     9961 ttgctggaaa cccgggctac tcgtatctta aacggtgagc gtggctctaa tgtgttggga
    10021 accatctatg aatggccgcc cagaaccagc agtgcgctgg atacgctgct tcgtgaaatt
    10081 cagaacgcac gcgaacaaca cagccgtctt gatacgctga tccgctctta tgccgcacag
    10141 gacgtgaaaa ccggcctcaa taaccgactc tttttcgata atcagttagc aacgttactg
    10201 gaagatcagg agaaagtagg tacccacggg atcgtgatga tgattcgtct gccggatttc
    10261 aatatgttga gcgatacctg ggggcacagc caggttgaag aacagttctt cactctgacg
    10321 aatctgctgt cgacatttat gatgcgctac cctggcgcac tgctggcgcg ttaccaccgc
    10381 agtgattttg ctgcgctgtt accgcaccgg acgttaaaag aggcagagag catagccagt
    10441 cagttaatca aagccgtcga taccttgccg aacaataaaa tgctcgatcg cgacgatatg
    10501 atccacattg gtatctgtgc ctggcgtagt ggtcaggata ccgagcaggt aatagaacat
    10561 gcagagtctg ccacgcgtaa tgcgggattg cagggtggca atagctgggc tatttatgat
    10621 gactcgttgc ctgaaaaagg acgcggtaat gttcgctggc gtacgcttat cgagcaaatg
    10681 ctcagtcgcg gcggcccgcg cctttatcaa aaaccggcgg ttactcgcga aggtcaggtt
    10741 catcatcgcg aactcatgtg ccgcatcttc gatggtaatg aagaggttag ctcggcggag
    10801 tatatgccga tggtcttgca gtttggctta tcggaagagt atgaccgtct gcaaatcagc
    10861 cgtcttattc cactattgcg ttactggcca gaggaaaatc tggcgattca ggttaccgtt
    10921 gagtcgctga ttcgtccgcg ttttcagcgt tggctgcgcg atacgttaat gcaatgtgaa
    10981 aaatcacaac gaaaacgcat aattattgaa cttgcagagg ccgatgtagg tcaacatatc
    11041 agtcgtttac aacctgttat tcgtttagtg aatgctttag gggtacgggt agccgtcaac
    11101 caggctggtt tgacgctggt aagcaccagt tggatcaaag aacttaatgt tgagttactc
    11161 aagctccatc cggggctggt caggaacatt gagaagcgaa cggagaacca gctgctggtt
    11221 caaagcctgg tggaagcctg ctccgggacc agcacccacg tttacgccac cggcgtgcgt
    11281 tcgcgaagcg agtggcagac cctgattcag cgcggtgtta caggcgggca aggggatttt
    11341 ttcgcgtcct cacagccact tgatactaac gtgaaaaaat attcacaaag atactcggtt
    11401 taacctgccg tttaatccgt tttcacgtag aataacgcgc gctgcgtctc atgggagtgt
    11461 gcttgtctgc tcgccagatt gttgcagcac atatgcagat gaatgacctt acgcggttgc
    11521 aaacaggcga ggaatgctgc tgatgcatta agcctttctg gactcaggca gagatttgta
    11581 acaaaggaaa cgaactgcac taattttcac cgtagcagat gatttttgcg ccttgtcgct
    11641 gctgcgtgtg gttggtaaag taagcggatt ttcttttccg ccccagcttt caggattatc
    11701 ccttagtatg ttgaaaaaat ttcgtggcat gttttccaat gacttgtcca ttgacctggg
    11761 tactgcgaat accctcattt atgtaaaagg acaaggcatc gtattgaatg agccttccgt
    11821 ggtggccatt cgtcaggatc gtgccggttc accgaaaagc gtagctgcag taggtcatga
    11881 cgcgaagcag atgctgggcc gtacgccggg caatattgct gccattcgcc caatgaaaga
    11941 cggcgttatc gccgacttct tcgtgactga aaaaatgctc cagcacttca tcaaacaagt
    12001 gcacagcaac agctttatgc gtccaagccc gcgcgttctg gtttgtgtac cggttggcgc
    12061 gacccaggtt gaacgccgcg caattcgtga atccgcgcag ggcgctggtg cccgtgaagt
    12121 cttcctgatt gaagaaccga tggctgccgc aattggtgct ggcctgccgg tttctgaagc
    12181 gaccggttct atggtggttg atatcggtgg tggtaccact gaagttgctg ttatctcctt
    12241 gaacggcgtg gtttactcct cttccgtgcg cattggtggt gatcgtttcg acgaagctat
    12301 catcaactat gtgcgtcgta attacggttc tctgatcggt gaagccaccg cagaacgtat
    12361 caagcacgaa atcggttcgg cttacccggg cgatgaagtc cgtgaaatcg aagttcgtgg
    12421 ccgtaacctg gcagaaggtg ttccacgcgg ttttaccctg aactccaacg aaatcctcga
    12481 agcactgcag gagccgctga ccggtattgt gagcgcggta atggttgcac tggaacagtg
    12541 cccgccggaa ctggcttccg acatctccga gcgcggcatg gtgctcaccg gtggtggcgc
    12601 actgctgcgt aaccttgacc gtttgttaat ggaagaaacc ggcattccag tcgttgttgc
    12661 tgaagacccg ctgacctgtg tggcgcgcgg tggcggcaaa gcgctggaaa tgatcgacat
    12721 gcacggcggc gacctgttca gcgaagagta atcggatgca ggcaggggaa gcgtctgttt
    12781 accctgcctg gtctgatacg agaatacgca taacttatga agccaatttt tagccgtggc
    12841 ccgtcgctac agattcgcct tattctggcg gtgctggtgg cgctcggcat tattattgcc
    12901 gacagccgcc tggggacgtt cagtcaaatc cgtacttata tggataccgc cgtcagtcct
    12961 ttctactttg tttccaatgc tcctcgtgaa ttgctggatg gcatatcgca gacgctggcc
    13021 tcgcgtgacc aactagaact tgaaaaccgg gcgttacgtc aggaactgtt gctgaaaaac
    13081 agtgaactgc tgatgcttgg acaatacaaa caggagaacg cgcgtctgcg cgagctgctg
    13141 ggttccccgc tgcgtcagga tgagcagaaa atggtgactc aggttatctc cacggttaac
    13201 gatccttata gcgatcaagt tgttatcgat aaaggtagcg ttaatggcgt ttatgaaggc
    13261 cagccggtca tcagcgacaa aggtgttgtt ggtcaggtgg tggccgtcgc taaactgacc
    13321 agtcgcgtgc tgctgatttg tgatgcgacc cacgcgctgc caatccaggt gctgcgcaac
    13381 gatatccgcg taattgcagc cggtaacggt tgtacggatg atttgcagct tgagcatctg
    13441 ccagcgaata cggatattcg tgttggtgat gtgctggtga cctccggtct gggcggtcgt
    13501 ttcccggaag gctatccggt cgcggttgtc tcttccgtaa aactcgatac ccagcgcgct
    13561 tatactgtga tccaggcgcg tccgactgca ggactgcaac gtttgcgtta tctgctgctg
    13621 ctgtggggcg cggatcgtaa cggcgctaac ccgatgacgc cggaagaggt gcatcgtgtt
    13681 gctaatgaac gtctgatgca gatgatgccg caggtattgc cttcgccaga cgcgatgggg
    13741 ccaaagttac ctgaaccggc aacggggatc gctcagccga ctccgcagca accggcgaca
    13801 ggaaatgcag ctactgcgcc tgttgcgccg acacagcccg ctgctaatcg ctctccacaa
    13861 agggctacgc cgccgcaaag tggtgctcaa ccgcctgcgc gtgcgccggg agggcaatag
    13921 tggcgagcta tcgtagccag ggacgctggg taatctggct ctctttcctc attgcgctgt
    13981 tgctgcaaat catgccctgg ccggataacc tgattgtttt ccggccaaac tgggtgttac
    14041 ttatcttgtt gtattggatc ctggccttgc cccatcgcgt aaatgtgggc acaggttttg
    14101 tgatgggtgc catactggat ctgatcagcg gctcgatgct tggcgtacgc gtattggcga
    14161 tgagcatcat tgcttacctg gtggcgctga aataccagct tttccgcaac ctcgcattat
    14221 ggcagcaggc gctggtcgtc atgttgcttt cgctggtggt ggatattatt gttttctggg
    14281 cagagttttt agtgattaac gtctctttca gaccggaagt gttctggagt agtgtagtca
    14341 atggggtgct ctggccgtgg attttcttgc tgatgcgtaa agtccgtcag cagtttgccg
    14401 tgcaataaag gtttctatga cttctctgta tttagcttcc ggttctccgc gtcgtcagga
    14461 gttacttgcg caacttggcg tgacctttga acgtattgtt acgggcattg aggagcagcg
    14521 tcagccgcag gagagcgcgc agcagtatgt tgtgcgtctg gcacgcgaga aagcacaggc
    14581 gggtgtcgcg caaacggcac aggatctccc ggtgctgggt gcggatatta tcgttatcct
    14641 gaacggagaa gtgctcgaga aaccgcgcga cgcagagcat gcggcgcaga tgttgcgcaa
    14701 attatcgggt cagacccatc aggtgatgac ggcagtggcg ttggccgaca gccagcatat
    14761 tctcgattgc ctggtggtca ccgatgtgac tttcagaacg ttaacagacg aagacatcgc
    14821 gggctatgtc gccagcggtg aaccgttaga taaagcaggt gcatacggta ttcaggggct
    14881 gggtggctgt tttgtcagga agataaatgg cagctatcac gccgtagtcg gcttaccgct
    14941 ggttgaaacg tatgaattat taagtaattt taacgcactg cgtgagaaaa gggataaaca
    15001 tgacggctga attgttagta aacgtaacgc cttcggaaac gcgagtggcg tatattgatg
    15061 gcggtattct gcaggaaatt catattgaac gtgaggcgcg acgcggaata gtaggcaata
    15121 tctacaaggg tcgtgtaagt cgtgtacttc cgggtatgca ggcggctttt gtagatattg
    15181 ggctggataa agccgcgttt cttcatgcat ccgacatcat gccgcatacc gaatgtgtgg
    15241 cgggtgaaga acaaaagcaa ttcacggtgc gcgacatctc ggaactggtt cgtcaggggc
    15301 aagatctgat ggtgcaggtg gtgaaagatc cgcttggcac taaaggtgcg cgcctgacca
    15361 ccgatatcac gctgccttct cgctatctgg tatttatgcc aggggcttct cacgttgggg
    15421 tttcccaacg aattgaaagc gaatcagaac gtgaacgcct gaaaaaagtg gtcgcagagt
    15481 attgcgacga gcagggcggg tttatcatcc gcaccgcagc ggaaggggtt ggcgaggctg
    15541 aactggcctc cgatgccgct tatctgaaac gcgtctggac caaagttatg gagcgtaaaa
    15601 agcgcccgca gacccgttat cagctgtacg gcgaactggc gctggcgcag cgtgttctgc
    15661 gtgattttgc cgacgccgaa ctggaccgca ttcgcgttga ctcacgcctg acttacgaag
    15721 cattgctgga gtttacctcg gagtacattc ccgagatgac cagcaagctg gagcattaca
    15781 ccggacgcca gccgattttc gatctctttg atgtcgaaaa cgaaatccag cgagcgttgg
    15841 aacgcaaagt agaactgaaa tccggtggtt atctgattat cgaccagacc gaagcgatga
    15901 ccaccgtgga catcaatacc ggagcgtttg tcggtcatcg caatctggac gacactattt
    15961 tcaataccaa tattgaagcg acgcaggcta tcgctcgcca gttacggttg cgtaatctgg
    16021 gcgggattat cattattgat ttcatcgata tgaataatga agatcaccgc cgccgcgtgc
    16081 tgcattcgct ggagcaggcg ttgagcaaag accgggtgaa aaccagcgtt aatggttttt
    16141 cggcgctggg gctggtggag atgacgcgta aacgcacccg cgaaagcatt gagcacgtac
    16201 tgtgtaacga atgcccaacc tgccacggtc gcggaacggt gaaaaccgtg gaaacggtat
    16261 gctatgaaat catgcgcgag attgttcgtg tccaccatgc ttacgactcc gaccgtttcc
    16321 tggtctatgc ttctccggca gtagccgaag ccttgaaagg cgaagagtca cactcgctgg
    16381 cggaagtgga aattttcgtt ggcaaacagg ttaaagtaca aattgaaccg ctctataacc
    16441 aggagcagtt tgacgtcgta atgatgtaaa cagatgctgg gccgccatcc ggcaaagggt
    16501 ttttgagtca catttttagc agacaaggag tgacgggtga ggcgattgcc ggggatttta
    16561 ctgcttactg gagccgcgct cgttgtgatc gctgccctgc tggttagcgg cctgcgtatt
    16621 gctttaccgc atcttgacgc ctggcgtccg gaaatcctca acaaaataga atccgcgact
    16681 ggcatgccgg tagaagccag tcagctctca gccagctggc agaattttgg cccgacgctt
    16741 gaagcacacg acatccgtgc agaactaaaa gatggcggcg aattttcggt taaacgcgtt
    16801 actctggcgc tggatgtctg gcagagcctg ttacatatgc gctggcagtt tcgcgacctc
    16861 actttctggc agctgcgctt tcgcaccaac actcctatca ccagcggtgg tagcgatgac
    16921 agtctggaag ccagtcacat cagcgatctg tttcttcgtc aatttgacca tttcgatctc
    16981 cgcgacagtg aagtcagttt tctgacgcca tccggtcaga gcgccgagct ggcgatccca
    17041 caactcacct ggctgaacga tccacgtcga caccgtgcgg aaggcctggt aagcctctcc
    17101 agccttaccg ggcagcacgg cgtgatgcag gtgcgcatgg atttgcgcga tgatgagggg
    17161 ttgttaagca atggtcgcgt ctggctacag gcggatgaca tcgacctgaa gccgtggctc
    17221 ggtaaatgga tgcaggacaa tattgctctg gaaacggcac agttctccct tgaaggctgg
    17281 atgacgatcg acaaaggcga tgtaaccggc ggtgacgtct ggctgaaaca gggcggtgcc
    17341 agctggttgg gcgagaagca aacgcatacg ctgtcggtgg ataatctgac cgcgcatatt
    17401 acgcgtgaaa atccgggctg gcagttctct attcccgata cacggatcac gatggacggc
    17461 aaaccctggc cgagcggagc attgacgctg gcctggatac cggaacagga cgttggcggc
    17521 aaagacaata aacgcagtga cgaactccgg attcgcgcta gtaatctgga gctggcaggc
    17581 ctggagggca tacgcccgct ggccgcgaaa ctttcacctg cactgggtga tgtttggcgc
    17641 tccacacaac cgagcggcaa gattaacact ctggcgctgg atatcccgct tcaggcggca
    17701 gacaagaccc gttttcaggc atcgtggagc gatctggcct ggaagcaatg gaaattatta
    17761 ccgggtgcgg aacacttctc cgggacgctt tccggcagcg ttgaaaatgg tttgcttacc
    17821 gcgtcgatga agcaggcaaa gatgccttac gaaacggtat tccgtgcgcc actggaaatc
    17881 gccgacggcc aggcaactat aagctggctg aacaatgaca aaggtttcca gctggatggg
    17941 cgtaatattg acgttaaagc caaagccgtc catgcgcgcg gcggttttcg ttacctgcaa
    18001 cctgctaacg atgaaccctg gctgggtatt ctggctggca tcagtaccga tgatggttca
    18061 caagcctggc gctatttccc ggaaaacttg atgggtaaag acctggttga ttatttaagt
    18121 ggtgcgattc agggcggtga agcggataac gcgacgctgg tttatggtgg caatccgcaa
    18181 ctcttcccct ataaacacaa cgaaggtcag tttgaagtgc tggtgccgct gcgcaacgcg
    18241 aagtttgcct tccagccgga ctggcctgca ttaactaacc ttgatattga actggacttt
    18301 attaacgacg gtttatggat gaaaaccgat ggcgttaatc tgggcggcgt gcgcgcgagt
    18361 aatctcaccg cagtgatccc tgactactca aaagaaaaac tgctgattga cgctgacatt
    18421 aaaggtccgg gtaaagccgt tggcccttac tttgatgaga caccgctgaa agattctctg
    18481 ggtgcgaccc tgcaagaact ccagctcgac ggcgatgtga atgctcgctt acatcttgat
    18541 atcccgctga acggcgaact ggtaaccgcg aaaggtgaag tgacgctgcg taataacagt
    18601 ctgtttatca aaccactcga cagcatcctg aaaaatttga gcggtaaatt cagctttatc
    18661 aatggcgatc tgcaaagtga accactgaca gcaagctggt ttaatcagcc gttgaacgtg
    18721 gatttttcca ccaaagaagg ggcaaaagcc taccaggttg cggtgaatct caacggtaac
    18781 tggcaaccgg cgaaaaccgg cgttctgcct gaagcggtga acgaagcatt gagtggcagc
    18841 gtggcgtggg atggtaaagt gggcattgat ctgccttatc atgctggcgc gacctataac
    18901 gtagagctga acggcgatct gaataatgtg agtagtcact taccttcacc gttagccaaa
    18961 cctgcgggtg aaccactagc ggtaaacgtt aaggttgatg gcaatctcaa cagctttgaa
    19021 ttaaccggac aggctggtgc ggataaccat ttcaatagcc gctggttgct tggtcaaaag
    19081 ctgacgctcg atcgtgctat ttgggcggca gacagtaaaa cgctcccgcc gttgccggaa
    19141 caaagtggcg ttgaacttaa tatgccgccg atgaatggtg ccgagtggct ggccctgttc
    19201 cagaaaggcg ctgcggagag tgtcggtggt gcagcgagtt tcccacaaca cataacatta
    19261 cgtacgccta tgttgtcact gggaaatcag caatggaata acctgagtat tgtttcgcaa
    19321 ccgacggcaa atggcaccct gattgaggcg caagggcgtg aaatcaacgc cacgctagcg
    19381 atgcgtaata acgcgccgtg gctggcgaat atcaaatatc tttattacaa cccgagcgtg
    19441 gcgaaagctc gtggtgattc aacgccgtca tcacctttcc cgacaacgga gcgcattaac
    19501 ttccgtggct ggccggacgc acaaatacga tgcacagagt gctggttctg ggggcaaaaa
    19561 ttcggtcgca ttgacagtga tatcaccatt tctggcgata cgttaacgct gaccaatgga
    19621 ctgattgata ctggtttctc gcggcttact gccgatggtg aatgggttaa taatccgggg
    19681 aatgaacgta cctcgctgaa aggaaaactg cgtgggcaga aaattgatgc cgccgcagaa
    19741 ttttttggtg tcacgacgcc catacgccag tcgtcattta atgtggatta cgatttacac
    19801 tggcgcaaag caccctggca gccagatgaa gcgacgttga atggcatcat tcatactcaa
    19861 ctgggtaaag gcgaaattac cgaaatcaat accggacatg ccgggcaatt gctgcgctta
    19921 ttgagcgtag atgccctgat gcgtaagctg cgttttgatt tcagagacac ttttggcgaa
    19981 gggttctatt ttgactccat tcgcagcacc gcgtggatta aagacggcgt tatgcacacc
    20041 gacgacacgc tggtggatgg cctggaggcg gatatcgcca tgaaagggtc ggtaaatctg
    20101 gtacgtcgag acctgaatat ggaagcggtt gtcgcaccag agatttctgc gacggtgggc
    20161 gtggctgcgg cttttgcggt taaccccatt gttggcgcgg cagtgtttgc cgccagtaaa
    20221 gtgctggggc cgctgtggag caaagtctcc attttgcgct atcacatttc gggtccgctg
    20281 gatgatccgc aaatcaacga agtgttgcgc caaccgcgta aagaaaaagc gcaatgattt
    20341 gacgagggcg cgtaattgcc ccaatctcat atgataatcg ttgccaaagg ccaacgagcc
    20401 ggaacataac tgtaggccgg atagggcgtt cacgccgcat ccggcagccg taaaaaatcc
    20461 tctactgcag taactaacga gtagcaaaaa cgatgagtct gaacctggta agtgaacaat
    20521 tgctagcggc gaacggcctg aaacatcagg acttgttcgc gatcctcggt caactggccg
    20581 aacgtcgcct tgattatggc gatctctatt ttcagtcgag ctatcacgaa tcctgggttt
    20641 tagaagaccg cattattaaa gatggttctt acaacatcga tcagggcgtt ggcgtgcgtg
    20701 caatcagcgg tgaaaaaacc ggatttgctt acgctgacca aatcagcctg ctggcgctgg
    20761 aacagagtgc gcaagcggcg cgcaccatcg tccgtgatag tggtgatggc aaagtacaga
    20821 cgctgggcgc ggtagagcat agcccgttgt atacctcggt agatccgctg caaagcatga
    20881 gccgtgaaga gaagctggat atcctgcgtc gcgtcgataa ggttgctcgc gaagcggaca
    20941 agcgcgtaca ggaagtgact gccagcctca gtggtgtcta tgaattaatt ctggttgcgg
    21001 ccaccgacgg cacgctagcg gcggatgtcc gtccgctggt gcgtctttcc gtgagcgttc
    21061 tggtcgaaga agatggcaaa cgcgaacgcg gtgccagtgg cggcggcggt cgttttggtt
    21121 atgagttctt ccttgccgat cttgacggcg aagtccgtgc ggatgcatgg gcaaaagaag
    21181 cagtgcgtat ggcgctggtc aatctttctg ccgttgctgc accagcgggc accatgccgg
    21241 tagtacttgg cgcaggttgg ccgggcgtgc tgttgcatga agcggtaggt cacggtctgg
    21301 aaggcgactt caaccgccgt ggcacttcag tatttagtgg acaggtcggg gagctggtgg
    21361 cttcagaact gtgtaccgtg gttgatgacg gcacgatggt cgatcgccgt ggttcggtgg
    21421 cgattgatga cgaaggtacg ccaggccagt acaacgtgct gattgagaac ggcattctga
    21481 aaggctacat gcaggataaa ctcaacgcgc gtttgatggg gatgacgccg actggcaacg
    21541 gtcgccgtga atcctacgcc catctgccca tgccgcgtat gaccaacacc tatatgctgc
    21601 cgggtaaatc gaccccgcaa gaaattattg aatccgttga gtacggtatc tatgcaccga
    21661 actttggtgg cggtcaggtg gatatcacct ccggcaaatt cgttttctcc acttcagaag
    21721 catatctgat tgaaaacggt aaagtaacga agccggtgaa aggcgcaacg ttgattggtt
    21781 ccggtatcga aaccatgcag cagatttcga tggttggcaa cgacctgaaa ctggataacg
    21841 gcgtgggtgt ctgcggtaaa gaagggcaaa gtttgccggt tggcgtgggc cagccaacgt
    21901 tgaaagtcga taacctgact gttggcggta ctgcgtaata atttcattat tttcagaagg
    21961 ataattaatc tttctacgtg cacgaacggt cccctcgctc ctctggggtt agggtgaggg
    22021 gaacccgttg gcacaggttt gtagaacgta acagtacaat atgaattact tctctttccc
    22081 gcgcccgtgc atctcctgaa acaatttacc gacctcaaca aaataatccg tcagcgagtt
    22141 gatcacgacc tgtaccttca gcggcagctt atctttttcg gtatataacg cataaaccgg
    22201 gcgtggatct gactgataac gcggcagcag gatctccagc tccccacgat tgatctcgtt
    22261 gatcacccac atcagcggca cgtaggcgat cccggcaccc gccgttagcc agcgcaccag
    22321 cgtcatcgga tcattagtca caaatctccc ttgcgggatc aggcgagtcg agatcccttc
    22381 cggtgcaatc agttcaaatt cattgtcggg ccgcacgctg tattcaagcc atgaatggct
    22441 acttaaatcg gcgggttttt ccggtatgcc gtattgtgtg agatagcttt tcgcggcgca
    22501 caccaccatt ggcatcgcgc caagacggcg ggaaaacagg ctggaatcct gcaacgcgcc
    22561 gacgcggatc accacatcca gaccgtcggc aatcaggtcg ggggctggaa ttccggtaac
    22621 cagattggcg ctcaaacctg ggtattcttt cagcattttg gctgtcagcc cggcgagaac
    22681 attttgtgcc atagttgaag aacagccaat gcgtagcgtc ccgatggggg tgttattgaa
    22741 ggcatacagt tgctcatgaa catcctgcac ttcatgaagc atacgacggc agccctggta
    22801 gtaaattcta ccggcttcgg tcaggccaat gctgcgtgtg ctacggttta acagctttac
    22861 ctgcaactca tcttccagtt ttgacaccgt ctgactgatg gacgaaacgc tcatctgtag
    22921 ctgtctggcg gcggcggtaa aagagccaaa ttcaactact ttggcaaaca ccgacatgcg
    22981 ttttagtcgt tccattattc actctgactt aaaagtgatt tagatcacat aatatagata
    23041 acagcataac agttacgcta atatattaaa tatcaatcta cagcaatgtt gctctcgccc
    23101 ggctttccat gcccttctct gcggcgacag atgctgaaaa taataacgcc tgctctctct
    23161 atacaaccaa ggtcaacatg agtctgtttc ccgttatcgt ggtgtttggg ctgtccttcc
    23221 caccgatatt ttttgaattg cttttatcac tggcgatttt ctggctggtg cgccgggtac
    23281 ttgtgccaac aggtatctac gactttgtct ggcatccggc gttgttcaac accgcgctct
    23341 attgctgctt gttttatttg atatcgcgac tgttcgtttg aggttgaagt gaaaacacta
    23401 ataagaaaat tctcccgtac ggccatcacg gtcgtattag tcattctggc cttcatcgca
    23461 atttttaatg cctgggtcta ttacaccgaa tccccctgga cgcgtgacgc gcgctttagc
    23521 gctgacgtcg ttgcgatcgc gccggacgtt tctggactca ttacccaggt gaatgttcat
    23581 gataaccagc tggtgaaaaa aggacagata ctgttcacca tcgaccagcc gcgctatcaa
    23641 aaggcgcttg aggaagcgca agccgatgtt gcttattatc aggtactggc acaggagaaa
    23701 cgccaggagg ccggacgtcg taaccgtctc ggtgtgcagg cgatgtctcg cgaagagatc
    23761 gaccaggcta acaacgtact acaaacggtt ctgcatcagt tagcgaaagc gcaggcgacc
    23821 cgcgatctgg caaaactgga tcttgaacgc acggtgatcc gagcgccagc agatggctgg
    23881 gtgaccaacc tcaacgtcta taccggtgag tttattactc gaggatcaac ggcggttgcg
    23941 ctggtgaaac agaactcctt ctatgtactg gcctatatgg aagaaactaa gctggaaggg
    24001 gtgcgtccgg ggtatcgtgc agagatcacg ccgcttggca gtaacaaagt gctgaaaggg
    24061 actgttgata gtgttgccgc tggggtcacc aacgccagca gcacgcgtga cgacaaaggg
    24121 atggcgacca tcgactctaa ccttgaatgg gtgcgtttgg cgcaacgtgt tccggttcgt
    24181 attcgtctcg acaaccagca agagaacatc tggcctgcgg gcaccactgc tacagtggtg
    24241 gtcactggca aacaagatcg cgacgaaagc caggattcgt tcttccgtaa aatggcccat
    24301 cgcctgcgtg agtttggtta atcacgatgg gtattttctc cattgctaac caacatattc
    24361 gctttgcggt aaaactggcg accgccattg tactggcgct gtttgttggc tttcacttcc
    24421 agctggaaac gccacgctgg gcggtactga cagcggcgat tgttgccgcc ggtccggcct
    24481 ttgctgcggg aggtgaaccg tattctggcg ctattcgcta tcgtggcttt ttgcgcatca
    24541 tcggcacatt tattggctgt attgccggac tggtgatcat cattgcgatg atccgcgcac
    24601 cattattgat gattctggtg tgctgtatct gggccggttt ttgtacctgg atatcctcgc
    24661 tggtacgaat agaaaactcg tacgcgtggg ggctggccgg ttataccgcg ctgatcattg
    24721 tgatcaccat tcagccggaa ccattgctta cgccgcagtt tgccgtcgaa cgttgtagcg
    24781 agatcgttat cggtattgtg tgtgcgatta tggcggattt gctcttttct ccgcgatcga
    24841 tcaaacaaga agtagatcga gagctggaaa gtttgctggt cgcgcaatat caattaatgc
    24901 aactctgtat caagcatggc gatggtgaag ttgtcgataa agcctggggc gacctggtgc
    24961 gacgcaccac ggcactacaa ggcatgcgca gcaacctgaa tatggaatct tcccgctggg
    25021 cgcgggccaa tcgacgttta aaagcgatca atacgctatc gctgacgctg attacccaat
    25081 cctgcgaaac ttatcttatt cagaatacgc gcccggaatt gatcactgat actttccgcg
    25141 aattttttga cacgccggta gaaaccgcgc aggacgtcca caagcagctc aaacgcctgc
    25201 ggagagttat cgcctggacc ggggaacggg aaacgcctgt caccatttat agctgggtcg
    25261 cggcggcaac gcgttatcag cttctcaagc gcggcgttat cagtaacaca aaaatcaacg
    25321 ccaccgaaga agagatcctg caaggcgaac cggaagtaaa agtagagtca gccgaacgtc
    25381 atcatgcaat ggttaacttc tggcgaacca cactttcctg cattctgggc acgcttttct
    25441 ggctgtggac gggctggact tccggcagtg gtgcaatggt gatgattgcg gtcgtgacgt
    25501 cactggcaat gcgtttgccg aatccacgca tggtggcgat cgactttatc tatgggacgc
    25561 tggccgcgct gccgttaggg ctgctctact ttttggtgat tatccctaat acccaacaga
    25621 gcatgttgct gctgtgtatt agcctggcag tgctgggatt cttcctcggt atagaagtac
    25681 agaaacggcg actgggctcg atgggggcac tggccagcac cataaatatt atcgtgctgg
    25741 ataacccgat gactttccat ttcagtcagt ttctcgacag cgcattaggg caaatcgtcg
    25801 gctgtgtgct cgcgttcacc gttattttgc tggtgcggga taaatcgcgc gacaggactg
    25861 gacgtgtact gcttaatcag tttgtttctg ccgctgtttc cgcgatgact accaatgtgg
    25921 cacgtcgtaa agagaaccac ctcccggcac tttatcagca gctgtttttg ctgatgaata
    25981 agttcccagg ggatttgccg aaatttcgcc tggcgctgac gatgattatc gcgcaccagc
    26041 gcctgcgtga tgcgccgatc ccggttaacg aggatttatc ggcgtttcac cgacaaatgc
    26101 gccgcacagc agaccatgtg atatctgccc gtagcgatga taaacgtcgt cggtactttg
    26161 gccagttgct ggaagaactg gaaatctacc aggaaaagct acgcatctgg caagcgccac
    26221 cgcaggtgac ggaaccggtt catcggctgg cggggatgct ccataagtat caacatgcgt
    26281 tgaccgatag ttaagtcaaa accgacgcca aaagcgtcgg ttttttcatg gctatactta
    26341 gcgatgcacg gcagaactcg ccgcgaaacg tgacggtggc aacagatgaa tatttatgcc
    26401 tttgatttta atgagattga gagtcaggag gatttttatc gtgactttag ccaaaccttt
    26461 ggtctggcta aagataaggt acgcgatctc gactcactat gggatgtgtt aatgaacgat
    26521 gtcctgccgc taccacttga gattgaattt gttcatctgg gagagaaaac gcgtcgccgt
    26581 tttggcgcgt taatattgct gtttgatgag gcagaggaag agctggaagg gcatttgcgt
    26641 tttaatgttc gtcattagcg caaaaaaaaa gcccccgaac cgggggcaat atcgtcggac
    26701 aaaacgatga gggtttattt gtacagttca gccgtagcgt gccaggtgtc accgctacga
    26761 gcttcagtaa tctggtaggg cgttgcgcct ttctcttccg cttttttgtt cagcatttca
    26821 cgtatatcca ttggcgaaga cgccacacca cttacggata cggtcccgat tgcttcacga
    26881 ttttgtgctt gtgcagcatc aatggagtcg gcagcgaatg caccgaaaga gagaacagaa
    26941 agtacgctta atgcagctac agtggttttg attttcatga tttttacctc gacataatct
    27001 tttagctggg cctttgtttc gtgacccata tcacaaaatc aagtacacac taattattga
    27061 actaattaat accacgctaa ctgtttcagt tgatactaag tgttaaaaat gtgaatttta
    27121 ataacaaaaa agtataaaaa acacggttat ttattattaa ttatagtatt ttcaattaga
    27181 taatgaattt tgcataatgt gcttttttta ccgtatgtat tcattttgtg aatgatgtgt
    27241 cgcggtaaaa cgcactattc gttaaatgta tcgcaggaga aagaagggga atgagaggga
    27301 taagcaacat tttccccgcc gccagaagcg acggggcaga gattaaagct cctggtcgaa
    27361 cagctctaaa atcgcttcgt acaggtcttt gacggtgaaa ccgttagcag gggtggtaaa
    27421 gatggtgtca tcgccagcga tggtgcccag aataccttct gctttgccca gtgagtccag
    27481 caggcgagca attaactgcg ccgcgccagg gctggtatga atcacgacaa ctgcatcgtt
    27541 gtagtcgata tccagcacca gattcttcaa tggactggag gtggttggta cacccagttc
    27601 agctggcagg cagtaaacca tttccatttt ggcattgcgt gtacgtacag caccaaactt
    27661 ggtcaacatc cgcgagactt tagactgatt aatattgtca aagccttgct cctgcaacgc
    27721 ggcgacgatt tcgccctggg agctaaattt ctcttcttta agtaatgctt taaatgcttt
    27781 aactagttct tcttgcttag ccgagcttcg cataagtcac ccgatatggt ggttgataca
    27841 acattattgt gcatacagat gaatttttat gcaaacagtc agccctgaag aaggctgaaa
    27901 taatgttatg aaagagcggg attttatcaa atttcgttat tgagaaacat gcctgcgtca
    27961 cggcatgcaa attctgttta aaagtaaatt aattgttatc aaattgatgt tgttttggct
    28021 gaacggtagg gtatattgtc accacctgtt ggaatgttgc gctaatgcat aagcgactgt
    28081 taattacgta agttaggttc ctgattacgg caattaaatg cataaacgct aaacttgcgt
    28141 gactacacat tcttgagatg tggtcattgt aaacggcaat tttgtggatt aaggtcgcgg
    28201 cagcggagca acatatctta gtttatcaat ataataagga gtttaggatg aaagtcgcag
    28261 tcctcggcgc tgctggcggt attggccagg cgcttgcact actgttaaaa acccaactgc
    28321 cttcaggttc agaactctct ctgtatgata tcgctccagt gactcccggt gtggccgtcg
    28381 atctgagcca tatccctact gctgtgaaaa tcaaaggttt ttctggtgaa gatgcgactc
    28441 cggcgctgga aggcgcagat gtcgttctta tctctgcagg cgtagcgcgt aaaccgggta
    28501 tggatcgttc cgacctgttt aacgttaacg ccggcatcgt gaaaaacctg gtacagcaag
    28561 ttgcgaaaaa ctgcccgaaa gcgtgcattg gtattatcac taacccggtt aacaccacag
    28621 ttgcaattgc tgctgaagtg ctgaaaaaag ccggtgttta tgacaaaaac aaactgttcg
    28681 gcgttaccac gctggatatc attcgttcca acacctttgt tgcggaactg aaaggcaaac
    28741 agccaggcga agttgaagtg ccggttattg gcggtcactc tggtgttacc attctgccgc
    28801 tgctgtcaca ggttcctggc gttagtttta ccgagcagga agtggctgat ctgaccaaac
    28861 gtatccagaa cgcgggtact gaggtggttg aagcgaaagc cggtggcggg tctgcaaccc
    28921 tgtctatggg ccaggcagct gcacgttttg gtctgtctct ggttcgtgca ctgcagggcg
    28981 aacaaggcgt tgtcgaatgt gcctacgttg aaggcgacgg tcagtacgcc cgtttcttct
    29041 ctcaaccgct gctgctgggt aaaaacggcg tggaagagcg taaatctatc ggtaccctga
    29101 gcgcatttga acagaacgcg ctggaaggta tgctggatac gctcaagaaa gatatcgccc
    29161 tgggcgaaga gttcgttaat aagtaattga ttagcgaata ataaaaaacc ggagcacaga
    29221 ctccggtttt tgttttgagc actcgactta attggttgcc ggatattcct gaatggtgac
    29281 ctgcagcgtt aactgcttat catcacgcat cactacaacc gggatcaccg aaccagggcg
    29341 aatttccgcc acctgatcca tcgtctccag agcagagatg gccggtttgt tatccaccga
    29401 aataatcaga tcgttgacct gaatacccgc attcgccgct gggccgtcag gtgacacttc
    29461 attaaccacg atcccttgca gttgatctat accaccgctc tgcgcgtgca gtggtgcgat
    29521 ctcgcgtccg ccgataccaa tgtagccgcg gatcacgcga ccatcgcgga tcagcttatc
    29581 cataattttg gttgctaact ggaaaggaat cgcaaagccg ataccttccg gcgtttcgcc
    29641 atcgttactc ttatcaaacg acagcgtgtt aatgcccatc agttctccca gcgagttcac
    29701 cagcgcgccg ccagagttac cgtggttaat ggaagcatcg gtttgtagga agttttgccg
    29761 cccggtcggg ttcagaccga ttcgacccgt ggcactaata atcccttggg taatggtctg
    29821 cccgaggttg tacgggttac cgatcgccag tactacgtcg ccaatgtgcg gtacgcgacg
    29881 tgcattaatt ggaatggtag gtaaaccgcc agtggcatta attttaagta ccgccagatc
    29941 ggttagagag tcagatccca ccagcaatgc ttcaaataca cgtccatcct gtaaggcgac
    30001 gatgatctga tcggcgtcgt tgatgacgtg tttattggtg atgatataac cgcgttgatc
    30061 catgattaca ccggatccca gggtgcggat ctcaagctgg ttgtgagagt tggtgttcaa
    30121 accacggttg taaacgttaa ccaccgctgg cgcggcgcgg cgaaccgcca gattatagct
    30181 ggcaggcgtc tcatcggtac tgtcaaattg cggagtggaa agcgggttaa ggctgcgcag
    30241 cgaaggcatg gcaaccagca gaatagcgcc gacaattaat ccaatcgcaa cggaacgtaa
    30301 gagcttcaca aacatgatgg aggcgtcatt aaaaaaggga acggcagcag cataccacga
    30361 gttaaccgga catcacacgt cagcctgatg cccggtttac gacattaacg catcagcaga
    30421 tagatgcttt cattgccgcg tacaatttgc agggcgatga tggccggttt tgccgccagc
    30481 actttacgca tttcagcaat cgagttcacc cgatcgcggt tgacgccaat gatcacatcg
    30541 tctttttgca agccagcctg agcagctggg cttcctttga caacttcatc tatcttaata
    30601 cctttgccgc catcttttag ctgaccatcg ctcaacgttg caccttccag cgctggcgtg
    30661 atcatttcag cgctggccga cgaagaggtg ctggtatcaa gcgtcacttc tacttccagt
    30721 ggtttgccgt tacgcagcag gccaagcttc actttcgtgc ccggctctgt agtcgcgata
    30781 cgagagcgca actcagcaaa gctattcagc ggtttgccgt tgaggctggt aataatatcg
    30841 cccgctttga cgcccgcttt cgctgagcca gaacttggca acacttcgct gacaaacgcg
    30901 ccacgctgca cgtcaaggtt gaaggctttg gcgatatcgg cactcatctc ggtgcctttg
    30961 atgcctaaca aaccgcgttt gatttcacca aagtcgataa gctgctgcgc cagtgttcgc
    31021 gccatattac tggggatggc aaatccaatc ccgacgctcc cgccgccagg cgcaaggatt
    31081 gcagtgttga tgccaattaa ctcaccgtta aggtttaata gtgcaccgcc ggagttaccg
    31141 cggttaatgg aagcatctgt ctggataaag ttttccagac cttcaagatt caacccgctg
    31201 cggcctaatg cggaaacaat gccagaggtg gcggtttgcc caaggccaaa tgggttaccg
    31261 accgctacgg caaaatcacc gacgcgcaat ttatcggagt cggcaatagc gatttgcgtt
    31321 aatttgctcg ggttttgaat ttgtaacagg gcgatatcgc tctggtcatc gctaccaatc
    31381 agttttgcat caaactcgcg cccatcattg agctgaatac tgattttctg tgcctgatta
    31441 atcacatggt tgttggtcag cacatagcct ttactggcgt tgatgatgac accggaacct
    31501 aaaccttcga agggttgtgc aggttgatcc ggtaaatcat caccaaaaaa ctttttgaat
    31561 tcttccggga ttttctgtcc ctgactggcc gttccttcca cccgtacgct caccactgcc
    31621 ggaagcactt tttccagcat tggagccaga ctggggagag gggcctgatc ggcaacctgg
    31681 cctggaatcg acgcgacggc ctgaaatgac gccgagagag ttaacccggc acttaacgct
    31741 aatgcactca acagctgggt ttgttttttc attattcctg ctctcgtacc tgaatgataa
    31801 gaaaagagat tcaaaacagt ttgatgttat tgaattttca gacggctaac aatgagacac
    31861 cagattaaat gatagtcggg gagggagaga agaggagggc gcaatggctg cgcccgaaaa
    31921 ataaattagt cgcgcttcgc gccagtacgc agcaggccgg atgcgccttc agaatagtcg
    31981 cgaggcatct gcaccggtgc ctgatcgttg ctggcttcag actctgccag acgattacgg
    32041 aacgggtttg cttcagcaga cagttccggc agcaggctgc tggagctttt tgccatgtgc
    32101 tgatacagct ggcgatagtc gtgcgccatg gtatccagta attccgcgct gcgggcaaag
    32161 tggctaacca gctcttcgcg atactcgtcc agttcagctt tattcttttc cagttcgtac
    32221 tgcaacgcct gttgctggcg tagtttacga ttaccaaaac gcatggccac agcaccaata
    32281 atgatgccga cgactaaccc aattagcgca tattcccagg tcatgaacat ctcccgttgt
    32341 cttttgtttc cgtagggtgt tggcttcagg ctccatgcct gcggctgatt atgccactat
    32401 aaccgttaat tccacagaag tggaatcccg actgcatatc gcgtagtgta gaacggcctt
    32461 tttttcgtca accgtgaaca acggcgcacc gattattcaa ggaataacaa taagatcatg
    32521 caaagcgtta ccccaacatc gcaatacctg aaggcgctca atgaaggcag ccatcaaccc
    32581 gacgacgttc aaaaagaggc cgtcagccgc ctggaaatta tttatcaaga actcatcaat
    32641 agcaggccac cagcccccag gacgagtggg ctaatggcgc gggtcggtaa gctgtggggt
    32701 aaacgcgaag acacaaagca tacgccagtg cgtggcttat atatgtgggg cggtgtagga
    32761 cgcgggaaaa cctggctgat ggaccttttc tatcaaagcc tgccgggaga gcggaaacag
    32821 cgcctgcact ttcaccgttt tatgctgcgg gtgcacgaag agctaactgc cttacagggg
    32881 cagaccgatc cgctggaaat tattgccgat cgctttaaag ccgaaactga cgtgctctgt
    32941 tttgacgaat tttttgtttc tgatattacc gacgccatgc tacttggcgg tctgatgaaa
    33001 gccctgttca cccgcggtat taccctggta gcgacgtcaa atattccgcc ggacgaactt
    33061 tatcgaaatg gcctgcaacg tgcgcgtttt ctgcctgcaa tcgatgccat taaacagcat
    33121 tgtgatgtaa tgaacgtgga cgctggtgtt gattatcgac tgcgtacact cactcaggcg
    33181 catctgtggc tttcgccact caacgatgaa acccgggcgc agatggataa actatggttg
    33241 gcgctggcgg gggtgaaacg agaaaattca ccgacgttag aaatcaacca tcggccattg
    33301 gcgacaatgg gcgtcgagaa ccagacgctg gcggtttctt ttactacgct gtgcgtcgac
    33361 gcccgcagtc agcatgacta tattgcgctc tcacgcctgt ttcacacagt aatgttgttt
    33421 gatgtgccag tcatgacgcg gttgatggag agcgaagcgc ggcgctttat tgcgctggtg
    33481 gatgagtttt acgagcgcca tgtcaaatta gtggtgagtg cagaagtgcc gctgtatgaa
    33541 atttatcagg gtgagcggct gaagtttgag ttccagcgtt gcctgtcacg tctgcaagag
    33601 atgcaaagcg aagagtatct gaagcgcgag catttggcgg gttaaaacct gccacaaatc
    33661 acaaaaaggg gtcgatcttt gaccccgact tctctataat cctgcgaccc cacgttacaa
    33721 gaaagttttt ttcccaaaac tttttgtgtg ctggcatagg ctattcgaag gggtaggttt
    33781 gccggacttt gtcgtgtgaa cctcaacaat tgaagacgtt tgggtgttca ccaacgtgta
    33841 actatttatt gggtaagctt ttaatgaaaa cttttacagc taaaccagaa accgtaaaac
    33901 gcgactggta tgttgttgac gcgaccggta aaactctggg ccgtctggct actgaactgg
    33961 ctcgtcgcct gcgtggtaag cacaaagcgg aatatactcc gcacgtagat accggtgatt
    34021 acatcatcgt tctgaacgct gacaaagttg ctgtaaccgg caacaagcgt actgacaaag
    34081 tgtactatca ccacaccggc cacatcggtg gtatcaaaca agcgaccttt gaagagatga
    34141 ttgctcgccg tcctgagcgt gtgattgaaa tcgcggttaa aggcatgttg ccaaaaggcc
    34201 cgctgggtcg tgctatgttc cgtaaactga aagtttacgc gggtaacgag cacaaccacg
    34261 cggcacagca accgcaagtt cttgacatct aatcgggatt ataggcaatg gctgaaaatc
    34321 aatactacgg cactggtcgc cgcaaaagtt ccgcagctcg cgttttcatc aaaccgggca
    34381 acggtaaaat cgtaatcaac caacgttctc tggaacagta cttcggtcgt gaaactgccc
    34441 gcatggtagt tcgtcagccg ctggaactgg tcgacatggt tgagaaactg gacctgtaca
    34501 tcaccgttaa aggtggtggt atctctggtc aggctggtgc gatccgtcac ggtatcaccc
    34561 gcgctctgat ggaatacgac gagtccctgc gttctgaact gcgtaaagct ggcttcgtta
    34621 ctcgtgacgc tcgtcaggtt gaacgtaaga aagtcggtct gcgtaaagca cgtcgtcgtc
    34681 cgcagttctc caaacgttaa ttggcttctg ctccggcaga aaacaatttg cgaaaaaacc
    34741 cgcttcggcg ggttttttta tggctaaatt ctgaatcagc gtaaaaaatg ggaagttgct
    34801 ttttgctgcc gtctgaaaga caggtaaaac aaaaaccaca tcgccaataa gggtctaagt
    34861 caactatttc agactaaagc gcatctcttt ttccccattt ccggcatcca ctcaccacaa
    34921 aggtcgcaaa atctggtaaa ctatcatcca attttctgcc caaatgtcgg gtattgctca
    34981 ttttttgttt gattttcgaa caaagagagt cgttccttat tgggtaacac aacttctgac
    35041 tggccacctg gtggctggta gcagtaaaaa ttctgactat acctggaggt tttcatggct
    35101 gtcgctgcca acaaacgttc ggtaatgacg ctgttttccg gtcctactga catctatagc
    35161 catcaggtcc gcattgtgct ggctgagaaa ggtgtaagtt tcgagatcga acacgtggaa
    35221 aaggacaatc cgcctcagga tctgattgac ctcaacccga atcagagcgt tccgaccctg
    35281 gtggatcgtg agctgaccct gtgggaatct cgcatcatta tggaatatct ggatgagcgt
    35341 ttcccgcatc cgccactgat gcctgtttac ccggtagctc gcggtgaaag ccgtctgtac
    35401 atgcatcgca tcgaaaaaga ctggtacacg ctgataaaca ctatcatcaa cggttcagct
    35461 tctgaagcag atgccgcacg taagcaactg cgcgaagaac tgctggcgat tgcgccggtc
    35521 ttcggtcaga agccgtactt cctgagcgat gagttcagcc tggtcgattg ctaccttgct
    35581 ccgctgctgt ggcgtctgcc gcaactgggc atcgagttca gcggcccggg tgcgaaagag
    35641 ctgaaaggct atatgacccg cgtctttgag cgtgactctt tccttgcttc tttaactgaa
    35701 gcagaacgtg aaatgcgtct gggccggagt taatctgtat ggatttgtca cagctaacac
    35761 cacgtcgtcc ctatctgctg cgtgcattct atgagtggtt gctggataac cagctcacgc
    35821 cgcacctggt ggtggatgtg acgctccctg gcgtgcaggt tcctatggaa tatgcgcgtg
    35881 acgggcaaat cgtactcaac attgcgccgc gtgctgtcgg caatctggaa ctggcgaatg
    35941 atgaggtgcg ctttaacgcg cgctttggtg gcattccgcg tcaggtttct gtgccgctgg
    36001 ctgccgtgct ggctatctac gcccgtgaaa atggcgcagg cacgatgttt gagcctgaag
    36061 ctgcctacga tgaagatacc agcatcatga atgatgaaga ggcatcggca gacaacgaaa
    36121 ccgttatgtc ggttattgat ggcgacaagc cagatcacga tgatgacact catcctgacg
    36181 atgaacctcc gcagccacca cgcggtggtc gaccggcatt acgcgttgtg aagtaataca
    36241 aaacaggccc aggcggcctg ttttgtcttt ttaatgactt aataaaatag cgtgattgtg
    36301 gcaatcatat tcaccacgaa accgactgcc atggggatcg ctgtccgctt caccaccgca
    36361 aaaggcgaaa cgcctgcaat tcctgacgta acgaccacca ccgcagtaat cggcgaaacc
    36421 gcgcgcgcta gcgtcgtggc aaaatgcatc ggcataatca ttacaaccgc tggtacatgt
    36481 agtccggctg cgatattcgg aataagactg gcaaatgaca taaacggcgc attgccagag
    36541 cccatcacaa tggcacaaat ggcaatgact agcgccataa taatcatcac gccaataccg
    36601 cccagaccag aatgctccgc acccctgata accgcatcaa cagtgccaat cgtcgttaag
    36661 cctttcgcaa atatttcacc cgcgaccacc agcgttacca cgttgacaaa ctgcgtaccc
    36721 atgccgtcaa aaaacgcctg cacatcgtcc attgtttcgc gcaagttatg cttgcggaag
    36781 aactcaacaa acatcgtcac cgtcaaactt agtaacatca ccaccaccag atgcagttct
    36841 gacttcatca gcccgacgtg ggcgaggaac agcgagccga gcatcaggat taacggcatc
    36901 acaggtaaaa tggcgtaata gagcggcggg acattatcga gagttttttg ctctgcctgt
    36961 tcgtgattga tatttttatc ttttttgtca aaagcgcgtt gcacgaaaaa gtgggagatc
    37021 gccaccgaga taatgacgca agaggcgacc ggaagctggt agtggaagaa gtatgtggca
    37081 attttcattc ccgcgacctg ggctgcaaaa atggagttcg tttccagaat cccccactca
    37141 atggacatcg tggttgcgat aaccgccacc gcagagaggc gacttactcc cagactcacc
    37201 agcgtcggaa ataaggtgac catcagcaac atacccagac cggaggcgct ggtaataaac
    37261 tgcgccatga tttggccgat gacgtaagtt gccgacagaa taatatacgg cgagcgaatg
    37321 agttttaacg ggcggcttag caagctcacc atcgcgcgac tggctcctat gcgctccatg
    37381 tagcgggcat aaccgcccac cgccataatc gacagcccca atccggcaat gcggttggac
    37441 agcatgcgca ggatttcatt ataaatgtcg acgatcaagt agccgctgct tttggcggga
    37501 tcgagtacac caccgaaccc taaccagact ccgcacatca tcaggaagac acctgcggtt
    37561 tgtagttttt caggatcaaa tagcccatcg taattaatac gatgacagat ataattatgc
    37621 cgaacatt
//
LOCUS       DADMEQ010000026        36162 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.026, whole genome shotgun sequence.
ACCESSION   DADMEQ010000026 DADMEQ010000000
VERSION     DADMEQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.026"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..298
                     /locus_tag="JNZ05_001365"
     CDS             <1..298
                     /locus_tag="JNZ05_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8564652.1"
                     /translation="FPVYQRLSAIDNEIHNSGRRSREIVIGIDNTYPTIIFDQLISLG
                     DKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELENLTISELPPL"
     gene            422..684
                     /locus_tag="JNZ05_001366"
                     /pseudo
     CDS             422..684
                     /locus_tag="JNZ05_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001451535.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(681..2159)
                     /gene="scpC"
                     /locus_tag="JNZ05_001367"
     CDS             complement(681..2159)
                     /gene="scpC"
                     /locus_tag="JNZ05_001367"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HAY8564653.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(2183..2968)
                     /gene="scpB"
                     /locus_tag="JNZ05_001368"
     CDS             complement(2183..2968)
                     /gene="scpB"
                     /locus_tag="JNZ05_001368"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HAY8564654.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(2979..3974)
                     /gene="meaB"
                     /locus_tag="JNZ05_001369"
     CDS             complement(2979..3974)
                     /gene="meaB"
                     /locus_tag="JNZ05_001369"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HAY8564655.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(3967..6111)
                     /gene="scpA"
                     /locus_tag="JNZ05_001370"
     CDS             complement(3967..6111)
                     /gene="scpA"
                     /locus_tag="JNZ05_001370"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HAY8564656.1"
                     /translation="MPNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLGKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKMDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMTEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRAARDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVAGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(6315..7208)
                     /locus_tag="JNZ05_001371"
     CDS             complement(6315..7208)
                     /locus_tag="JNZ05_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HAY8564657.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            7591..8250
                     /gene="rpiA"
                     /locus_tag="JNZ05_001372"
     CDS             7591..8250
                     /gene="rpiA"
                     /locus_tag="JNZ05_001372"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAY8564658.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            8506..9738
                     /locus_tag="JNZ05_001373"
     CDS             8506..9738
                     /locus_tag="JNZ05_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAY8564659.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            9967..10026
                     /gene="ibsC"
                     /locus_tag="JNZ05_001374"
     CDS             9967..10026
                     /gene="ibsC"
                     /locus_tag="JNZ05_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HAY8564660.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            10359..10418
                     /locus_tag="JNZ05_001375"
     CDS             10359..10418
                     /locus_tag="JNZ05_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY8564661.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(10515..11117)
                     /locus_tag="JNZ05_001376"
     CDS             complement(10515..11117)
                     /locus_tag="JNZ05_001376"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAY8564662.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(11138..11321)
                     /gene="ssrS"
                     /locus_tag="JNZ05_001377"
     ncRNA           complement(11138..11321)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="JNZ05_001377"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(11363..11692)
                     /gene="zapA"
                     /locus_tag="JNZ05_001378"
     CDS             complement(11363..11692)
                     /gene="zapA"
                     /locus_tag="JNZ05_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAY8564663.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            11860..12438
                     /gene="ygfB"
                     /locus_tag="JNZ05_001379"
     CDS             11860..12438
                     /gene="ygfB"
                     /locus_tag="JNZ05_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HAY8564664.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            12464..13789
                     /gene="pepP"
                     /locus_tag="JNZ05_001380"
     CDS             12464..13789
                     /gene="pepP"
                     /locus_tag="JNZ05_001380"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAY8564665.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHARGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            13786..14964
                     /gene="ubiH"
                     /locus_tag="JNZ05_001381"
                     /gene_synonym="visB"
     CDS             13786..14964
                     /gene="ubiH"
                     /locus_tag="JNZ05_001381"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HAY8564666.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            14987..16189
                     /gene="ubiI"
                     /locus_tag="JNZ05_001382"
                     /gene_synonym="visC"
     CDS             14987..16189
                     /gene="ubiI"
                     /locus_tag="JNZ05_001382"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HAY8564667.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLSEWLR"
     gene            complement(16263..16346)
                     /locus_tag="JNZ05_001383"
     CDS             complement(16263..16346)
                     /locus_tag="JNZ05_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564668.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            16637..17731
                     /gene="gcvT"
                     /locus_tag="JNZ05_001384"
     CDS             16637..17731
                     /gene="gcvT"
                     /locus_tag="JNZ05_001384"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HAY8564669.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            17755..18144
                     /gene="gcvH"
                     /locus_tag="JNZ05_001385"
     CDS             17755..18144
                     /gene="gcvH"
                     /locus_tag="JNZ05_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HAY8564670.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            18263..21136
                     /gene="gcvP"
                     /locus_tag="JNZ05_001386"
     CDS             18263..21136
                     /gene="gcvP"
                     /locus_tag="JNZ05_001386"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HAY8564671.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEEIGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            21403..22146
                     /locus_tag="JNZ05_001387"
     CDS             21403..22146
                     /locus_tag="JNZ05_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY8564672.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGRSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(22203..23636)
                     /locus_tag="JNZ05_001388"
     CDS             complement(22203..23636)
                     /locus_tag="JNZ05_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY8564673.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            23681..23992
                     /locus_tag="JNZ05_001389"
     CDS             23681..23992
                     /locus_tag="JNZ05_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAY8564674.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLPELKKVIADIYPGQTQFYMIEFKC
                     L"
     gene            24156..24815
                     /locus_tag="JNZ05_001390"
     CDS             24156..24815
                     /locus_tag="JNZ05_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAY8564675.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(25011..25991)
                     /gene="ygfZ"
                     /locus_tag="JNZ05_001391"
     CDS             complement(25011..25991)
                     /gene="ygfZ"
                     /locus_tag="JNZ05_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HAY8564676.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            26234..26500
                     /gene="sdhE"
                     /locus_tag="JNZ05_001392"
     CDS             26234..26500
                     /gene="sdhE"
                     /locus_tag="JNZ05_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HAY8564677.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            26481..26888
                     /locus_tag="JNZ05_001393"
     CDS             26481..26888
                     /locus_tag="JNZ05_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564678.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(26928..27449)
                     /gene="fldB"
                     /locus_tag="JNZ05_001394"
     CDS             complement(26928..27449)
                     /gene="fldB"
                     /locus_tag="JNZ05_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HAY8564679.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            27561..28457
                     /gene="xerD"
                     /locus_tag="JNZ05_001395"
     CDS             27561..28457
                     /gene="xerD"
                     /locus_tag="JNZ05_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAY8564680.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLLMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            28482..29192
                     /gene="dsbC"
                     /locus_tag="JNZ05_001396"
     CDS             28482..29192
                     /gene="dsbC"
                     /locus_tag="JNZ05_001396"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HAY8564681.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            29198..30931
                     /gene="recJ"
                     /locus_tag="JNZ05_001397"
     CDS             29198..30931
                     /gene="recJ"
                     /locus_tag="JNZ05_001397"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAY8564682.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            31022..32120
                     /gene="prfB"
                     /locus_tag="JNZ05_001398"
     CDS             join(31022..31096,31098..32120)
                     /gene="prfB"
                     /locus_tag="JNZ05_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAY8564683.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            32130..33647
                     /gene="lysS"
                     /locus_tag="JNZ05_001399"
     CDS             32130..33647
                     /gene="lysS"
                     /locus_tag="JNZ05_001399"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAY8564684.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(33690..34238)
                     /gene="idi"
                     /locus_tag="JNZ05_001400"
     CDS             complement(33690..34238)
                     /gene="idi"
                     /locus_tag="JNZ05_001400"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAY8564685.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARILLSAFTQLK"
     gene            34293..34364
                     /locus_tag="JNZ05_001401"
     CDS             34293..34364
                     /locus_tag="JNZ05_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564686.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(34361..34486)
                     /locus_tag="JNZ05_001402"
     CDS             complement(34361..34486)
                     /locus_tag="JNZ05_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564687.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(34488..35936)
                     /locus_tag="JNZ05_001403"
     CDS             complement(34488..35936)
                     /locus_tag="JNZ05_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAY8564688.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         8678 a         9127 c         9703 g         8654 t
ORIGIN      
        1 gttcccggtt tatcaacgac tttctgcgat tgataatgaa atccataact cagggcgtcg
       61 ttcacgagaa attgtgattg gcatagataa tacttatccg acaattattt tcgatcagtt
      121 gattagtctc ggagataagt atgaaggcgt gaccgcccag ccagttgagt tcagcgaaaa
      181 cggcgttatc gataatcttt ttgaccgtca gttagatttt attatctccc cacagcatgt
      241 atctgcacga gttcaggaac tggaaaacct gactatcagt gaattaccac cgctgtgatt
      301 gggctttctc gtttctcgcc gttataaaga gcgtcaagag caagagctgc ttcaggagct
      361 gccctggcta caaatgcgct ttcaaaaccg ggctaacttc gaagcaatga ttgatgcgaa
      421 tatgcgacct tgtgggatca atcccaccat tatttatcgt ccctacagtt ttatggccaa
      481 aatcagcgcc gtggagcgcg gacattttct gacggtgatc ccgcattttg cctggtgact
      541 ggtgaatccg gcaacgctga aatattttga tgcgccgcac aggccgatgt atatgcagga
      601 atatctttat tcaatcagaa atcatcgtta taccgccacg atcttcagca tattgctgaa
      661 gatcgtgacg ggacaaacca ttaacccagc atcgagccgg ttgcaattaa attacggtgt
      721 aagtcgaaga cgtggctaag atcgtggtga atatgtccgc cgggcgcatt ttccagatag
      781 cgatgcagat aatcccgata cataggatgt gcacaattat caatgatagt gcgggcgcgt
      841 tgaagcgggg aaagaccgcg cagatcggcg atcccttgtt cggtgatgat cactttgacg
      901 ctgtgttcgc tgtgatcaac atggctgcac attggcacga cggttgagat cttgccttct
      961 ttagcaatcg acggagccat aaagatcgac agatacgcgt tgcgttcaaa atcaccgctg
     1021 ccgccgatgc cgttcatcag atcgaccccg gctacgtgcg ttgaatttgc atgcccgtaa
     1081 atatcaaact ccaggccgac gttcagagcg atgacgccca gacgacggat gatttccggg
     1141 ttattggaaa tctcctgcgg acgcaacaca atgcggctgg caaagtaatc catattgtcg
     1201 taaatcttgc gcagggaatc ggccgagatt gtcaggctgg aggcgctggc cccgctgatt
     1261 ttgccggttt ccagtaaatg caccaccgat tcctgtagca cttccgaata catcatgaac
     1321 ggaggaattt ccgggttttc ccccagacgc gccattaccg cattattgat attgcccacg
     1381 ccactttgca gcggcagaaa ttccggcgga atacgcccat gcgccatttc ctgcaataag
     1441 aacgtgacca cgttatcggc aatctgctgg cacatgggat tttgcttatc cagcatatta
     1501 ccggcgtcgg gcaagttggt ttccacgacg gcgacaatct ttttcggatc gatttgcaca
     1561 tagcgggtac cgacgcgatc cattgcatgg aagatcgaca cgctattgcg ccgcggtggc
     1621 gcgccaggaa tcacaatatc tgccagttct gcaacgcgcg gatcgtgata gtgattaagt
     1681 tcaatgatca ctttcttcgc tcgcagcagc caggtcggcg cattaccgat cccgctggtt
     1741 aaccagactc gaccatccgg tgccagtgcc gatgcttcaa tgacggcaac atcaatgtcg
     1801 ccgaagaaac cgtaattgac catttgcgcc acttcgctca aatgtaggtc aacgaaactc
     1861 accgcgccct gattgatctt tttacgtaaa ctggacgatg tttgatatgg cgcacgccag
     1921 gaaacagcat cggcgtcaga aagtacatcg tcagcggcgg cgctgattga cgcccccgtc
     1981 agcaggcgaa tttgatacgg ctttttggcc tcatgctgtt cgttagctct gcgggcaatc
     2041 gcggcgggta gggctttcgg cgaacccgct ggggtaaagc cgctaaatgc caccatgtcg
     2101 ttatgctgga taatttctgc cgcttcatcg gcggtcatcc ttgtccactg agtttccatt
     2161 tttactcctt cgttcgcagg gattaatgac caacgaaatt aggtttacgt ttttcgagga
     2221 aagcgttcat cccttcctgg taatcttcgc tgtcataaac cgcgcggcgc atcccctgaa
     2281 tacgttcaaa ttcatcggag ttcatggtgt gtgcttcgcc cagtacacgc agctcttctt
     2341 tgataacggc aatggctaac ggcgctttct cagagatgtg gtgcgccatt tgtaaggtga
     2401 aatcttccag ttcttccact tccacaacat ggttgaggat gccgacagcc agcgcgcgct
     2461 gggcggtgat tggcgaagcg gtaaaaatca gctctttgac aatgtggaag cccgcgtcgc
     2521 gggtcaggtt gtgaatgccg actaggttat acgggacgcc gaggtttaca ggcgtcattg
     2581 agaaggttga ggtactggcg gcgatgatca gatcggaact catgatcatt tcaaatgcgc
     2641 caccccaaac actaccttcc accatcgaaa tgatcggttt cgggaatttt tggatcatgc
     2701 gggtgatttg acgcaatgga tcatcatagg agagcggatc gcgaccgcca gacggcagtt
     2761 cgtgaatatc gtgacctgcg gagaagactt tggatccact tggcgcccgc aaaatgatac
     2821 agcgaatttc cggccggttg agatcgctta acgcctgcat aagatcatca ataaagactt
     2881 tacttaaggc attaagtttt cgtccatagt taaactcaat gaccgccact ttgttgatag
     2941 tggcaacgtt cacatactga taagacataa aaattccttt aatcaaaata ttgcgtctgg
     3001 ataaattcac tgagctgccg caggccggtg cgcggtgaga gcgtattgtt tttgaccgct
     3061 aaaagcgtct ggcggtaata gcgatcgaaa tcttcattcg cgaacaggtg attcagtact
     3121 tcttcttcgg tctgcttacg cagccattcc accgattgtt gttgccgcac ttgttgtaaa
     3181 cgaccactgg cagttagcgc ggttttgaag tcgatgatgg cgtgccagat ctcatcgatt
     3241 ccacgttttt ccagtgcgct acaagtcaga ccccgtggct gccattcgtc gtatttacgt
     3301 cgcagaatat gcagggcact ctcgtacata tgccgggcaa tggcgacatt ggtatggtta
     3361 tcgccatcgt ctttattgat aacgagcaga tcagccactt ccatcagccc ttttttaatg
     3421 ccctgcagat catcgccgcc accggcaatt tgcaacgaga taaaacagtc caccatgcgg
     3481 gcgacttccg tttccgactg cgcgacgcca accgtttcga caatcactac gtcataaccc
     3541 gccgcttcgc ataacagcat taattcccgt gctcgctgac tggcaccgcc cagatgaccg
     3601 gaggagggta ccgggcgaat aaacgccgcc tcggcacgcg ccaggtcatt catgcgggtt
     3661 ttatccccga gaatgctacc gccagtgacc gggctgctgg gatcgaccgc aataacagca
     3721 acctttaatc cctcacgaat caacaacatg ccaaaggctt caagaaaggt acttttcccc
     3781 gcgccgggtg tgccggtaac gcccagtcgc agggcgttac cacagaacgg cataatagcg
     3841 tcaagcagct gtgtacttag cgcctgatga cgtgggtgac ggctttccac cagcgtcatg
     3901 gcttgggcga gcgtggcacg ctcaccctga cgtaagcggc gaatactttc tgccagcgtg
     3961 gcttcattaa tcatgatgct ggcttatcag attcagtacg tcgcgcacac tgtcgagcat
     4021 aggtgtacct ggaccataaa tcgccgccac gccgcgctct tgcaggaagg cgtaatcctg
     4081 cggcggaatg acgccacccg cgaccacgca gatatcttcg cgtccccatt ttttcagcgc
     4141 ttcgaccagt tccgggatca gcgttttatg accggcagcc agtgaggatg cgcccgctac
     4201 gtgaacgtcg ttttctacgg ccaggcgggc gatctcttca ggtgtagaga acatcgggct
     4261 taaatctacg tcgaaaccga gatcggaata ggcgctggcg atcactttcg cgccgcgatc
     4321 gtgtccatcc tggcccatct tagcgatcag aatgcgcggg cgacgaccat tgtcggcaag
     4381 gaactgctcc gtttgcgcaa caatggcatc gaactcggag gccgatttct cagactgatg
     4441 atagctttgc gcaatcacgc cggtaacaca ctggcttggc accagataac ggtcgaaagc
     4501 gacttccagc gcatcggaaa tttcacccag ggtggcgcga acgcgagcgg cattaacagc
     4561 ggcagccagc aggttttcgt tatgctgtgc ggcgtgagtc agggcgttca acgcggcggt
     4621 tacggcggca tcatcacggg cggcgcgaat gcgttccagc gaagcaattt gctcgttacg
     4681 caccatcacg ttgtcgatct caagtacatc ggtttcgtct tcgtgatcca gtttgtactt
     4741 gttgacacca acgatgacac gcttgccctg gtcgatcagc gactgttcgc gcgctgaggc
     4801 ctcttcggtc attcgttttg gcagacctgc ttcgatcgct ttcgccatgc caccggcttc
     4861 gtcgatctgt tggataatag ctctggcttg tttgacgatt tgatcggtca gcgactcaat
     4921 gtaataggat ccggccagtg gatcgacggt gtggcagagt tctgattctt cctggatgat
     4981 gatctgggtg ttgcgggcaa tgcgtgctga gaaatcggta ggcaaaccaa gcgcttcgtc
     5041 aaaggcgttg gtatgcagtg actgagtacc gcccagcgtc gcagccagtg cttcaatggt
     5101 ggtgcggata acgttgttat acggatcctg ttcagtcagg ctccagcctg aggtctggca
     5161 gtgggtacgc agcgccagtg atttcggatc ctgtgcgcca aatccactga ccgcttcgct
     5221 ccataaataa cgtgccgcac gcaacatggc gacgttcata aacagatcca tgccgatacc
     5281 gaagaataac gacaggcgag gagcgaagtc atccattttc agtccggcag agattgctgc
     5341 tttgatgtac tcaatcccat cggcgagcgt aaatgctacc tgctgcacgc agttggcacc
     5401 cgcttccccc atgtggtaac cgctgatact gatggtatta aatcgcggca tgttgccgga
     5461 acaccaggcg atgatgtcgg cgataatgcg cattgacggt tttggtgggt aaatataggt
     5521 gttgcggcag aggtactctt tgagaatatc gttttgaatg gtgccggtca gtttatcagg
     5581 tgtaacacct tgctcttctg cggcgacgat ataaaacgcc agtactggta gcactgcgcc
     5641 attcatggtc atcgaaaccg acattttacc cagcgggatc tggtcgaaca ggactttcat
     5701 atcttccacg gtgtcgatag cgacgcccgc tttgccgacg tcacccgcca cgcgtgggtt
     5761 atcggagtcg tagccacggt gggtggcaag gtcaaacgca acggaaagac ctttttgccc
     5821 ggcggccagg ttacggcgat aaaaagcgtt ggactctttt gctgttgaaa aaccagcata
     5881 ctgacggatg gtccacggtt gggcggtata catagtggca cgcgggccac gaacgtaggg
     5941 cggcaaacca ggaagggtac ctgtcacctc cagattatcg agatcggctt cggtatacag
     6001 cggcttgatg gcgatccctt ccgcggtttg ctgaaccagc gagtcgacag ttttctcccg
     6061 acggctcaat tccttgttgg caagctgttg ccactcctgc tcgttaggca ttatttgctc
     6121 cgtaaaacgc aatcaaaatg agggtatttg cgcttacggt gagcttttta cgtgggtttg
     6181 tcgccagcaa aaaaaatgca ttcagacgtc gttttttgcc gcctgagatt tgtctatttt
     6241 gtgatgtgat tcaaggaata caaaaagaag tgaggacaac gcctgatatg tgctatcagg
     6301 cattatttga tggattaatc ctgacgaagg actttgtgac cataatcgag taacgcatca
     6361 gtgactttac gcatcatgcg gctttcagga gcaaagcggt gccagtagag catccgtcgt
     6421 tgaaatagcc caggcgttaa gtcaatcagt tcaccgctgg ccagctcttt ctcgatttgc
     6481 aggtgcggga tcatacagca ggtggtgccc tggcgagcaa gttgtacgaa cgcttctgaa
     6541 gaattaacga tatggcaggg tacgctgcca ggaggaagat cgaagttttg ctgcaaaaag
     6601 gcctggtgca tatcgtcaag atggtcaaac gcgaccactg gcgctttcaa taatgccgaa
     6661 cgcgttacgc cgttaggaaa atatttttcg gcaaacggtt ttgagctgac gaacagatag
     6721 tcgagcgcac cgagtttatc gacaagacaa ctcggcagcg cctgatgttg aatactcacc
     6781 gcgccgacca cttcgccgcg gcgcagacgt tcctgagtac gggtttcatc ttctacctgc
     6841 aagttgaggc ggataggcga atcagccaac acaggagcca gtgccggaag caaccacgtc
     6901 gccagactgt cggcgttgac cgccagtgaa agcagcagcg gagtcgaacc ggtttgttca
     6961 tcgcccagcc actcttcttc cagcaactcc acctggcgca gcagtgccag cagtttttgc
     7021 ccttgttccg tcgggcgcgg cggtacggta cgcaccaaca gcggctgccc gaacatattt
     7081 tccagttgct taatgcgctg tgagacggct gattgtgtaa tgcacagctt ttgtgccgcg
     7141 cgctcaaatc ctcgttcacg tatcaccgca tccagtgcct gtaatgttct gtagtccggg
     7201 cgtttcattg ctgttgtctg ctcctggtaa atgctttttc ctgcactatg acacaatttt
     7261 gtgtcaggtt gcatataccg aagccgtcgg cgggtaacat ttgtatcaat ggttaaacgc
     7321 gcagcttaaa agatcaggga tttgcgctat tgcatttcgc ttagcgactt cgcgccctgg
     7381 ttatgttgaa tggcgtggcg ttattgcctc tgttcgcacc gtggagcggc atacccagac
     7441 cgttgtatgc tttatttgcg gggcgaaagg ggatgcccgc cattgcgcga tattttatgg
     7501 atgagttaac cacccggctt gccaacgggg tctgaatcgc tttttttgta tataatgcgt
     7561 gtgaaatttc ataccacagg cgaaacgatc atgacgcagg atgaattgaa aaaagcagta
     7621 ggatgggcgg cacttcagta tgttcagccc ggtaccattg ttggtgtagg tacaggttcc
     7681 accgccgcac actttattga cgcgctcggc acgatgaaag gccagattga aggggccgtt
     7741 tccagttcag atgcttccac tgaaaaactg aaaagcctcg gcattcacgt ttttgatctc
     7801 aacgaagtcg acagccttgg catctacgtt gatggcgcag atgaaatcaa cggccacatg
     7861 caaatgatca aaggcggcgg cgcggcgctg acccgtgaaa aaatcattgc ttcggttgcg
     7921 gaaaaattta tctgtattgc cgacgcctcc aagcaggttg atattctggg taaattcccg
     7981 ctgccagtag aagttatccc gatggcacgt agtgcagtgg cgcgccagct ggtgaaactg
     8041 ggcggtcgtc cggaataccg tcagggcgtg gtgaccgata atggcaacgt gatcctcgac
     8101 gtccacggca tggaaatcct tgacccgata gcgatggaaa acgccataaa tgcgattcct
     8161 ggcgtggtga ctgttggctt gtttgctaac cgtggcgcgg acgttgcgct gattggcaca
     8221 cctgacggtg tcaaaaccat tgtgaaatga tctgacgggg gaacctcccc cgttaaaaaa
     8281 attctcttca ttaaatttgg tgacatgtgt cacgctttta ccaggcaatt gtcgattgct
     8341 ctaaataaat cctctaaacc agcatattca tccaagaatt acctttgcgt gatatttcct
     8401 caacatcgcg acgcaaacgt tcatattgcc gcaatattat tttttgatat gttgaaaggc
     8461 ggatgcaaat ccgcacacaa catttcaaaa gacaggattg ggtaaatggc aaaggtatcg
     8521 ctggagaaag acaagattaa gtttctgctg gtagaaggcg tgcaccaaaa ggcgctggaa
     8581 agccttcgtg cagctggtta caccaacatc gaatttcaca aaggcgcgct ggatgatgaa
     8641 caattaaaag aatccatccg cgatgcccac ttcatcggcc tgcgatcccg tacccatctg
     8701 actgaagacg tgatcaacgc cgcagaaaaa ctggtcgcta ttggctgttt ctgtatcgga
     8761 acaaaccagg ttgatctgga tgcggcggca aagcgcggga tcccggtatt taacgcaccg
     8821 ttctcaaata cgcgctctgt tgcggagctg gtgattggcg aactgctgct gctattgcgc
     8881 ggcgtgccgg aagccaatgc taaagcgcac cgtggcgtgt ggaacaaact ggcggcgggt
     8941 tcttttgaag cgcgcggcaa aaagctgggt atcatcggct acggtcatat tggtacgcaa
     9001 ttgggcattc tggctgaatc gctgggaatg tatgtttact tttatgatat tgaaaacaaa
     9061 ctgccgctgg gcaacgccac tcaggtacag catctttctg acctgctgaa tatgagcgat
     9121 gtggtgagtc tgcatgtacc agagaatccg tccaccaaaa atatgatggg cgcgaaagag
     9181 atttcgctaa tgaagcccgg ctcgctgctg attaatgcct cgcgcggtac tgtggtggat
     9241 attccggcgc tgtgtgatgc gctggcgagc aaacatctgg cgggggcggc aatcgacgta
     9301 ttcccgacgg aaccggcgac caatagcgat ccatttacct ctccactgtg tgaattcgac
     9361 aacgtccttc tgacgccaca cattggcggt tcgactcagg aagcgcagga gaatatcggc
     9421 ttggaagttg cgggtaaatt gatcaagtat tctgacaatg gctcaacgct ctctgcggtg
     9481 aacttcccgg aagtctcgct gccactgcat ggtggtcgtc gtctgatgca catccacgaa
     9541 aaccgtccgg gcgtgctaac tgcgctgaac aaaatcttcg ccgagcaggg cgttaacatc
     9601 gccgcgcaat atctacaaac ttccgcccag atgggttatg tagttattga tattgaagct
     9661 gacgaagacg ttgccgaaaa agcgctgcag gcaatgaaag ctattccggg taccattcgc
     9721 gcccgtctgc tgtactaatt ccccttctct gaaaattaac gggcaggtca ctgacttgcc
     9781 cgttttttta tcccttctcc acaccgttgc cgcgttgcgc attctacttt gcgagtctgc
     9841 tccagaaaat aaatcaaagt cagatcaatg cgttgtgttt atttaatggg gatttacgat
     9901 ggcgggacag catggggctg taacggtaaa gccctcaccg aagcgagggc ttaaaggaga
     9961 agggttatga tgcgacttgt catcatactg attgtactgt tactgataag tttcggagct
    10021 tattaacagt cagtctcaag ggaggagtca tcctccctta cccttactca ctaaattagg
    10081 tcaaagaatc aacgatgtca atcagggcga tgcggggtgc atcgccctga accgaacttt
    10141 ttgatagcca cttcatcgcc cacgtttact gccgtaatag atcctttttc ccaaaaatta
    10201 tctctgtttt gcatggggtt tttcgagcac gctcgaagaa aagatttaaa ggtagatcaa
    10261 tgcgttgtgg ttatttaaag agagcttacg atagctgggc agcatggggc tgtaacggta
    10321 aagccctcac cgaagcgagg gcttgaagga gaagggttat gatgcgactt gccatcatac
    10381 tgattgtact gttactcata agtttcccag cttattaaca gtcacagggg aggagcaatc
    10441 ctcccttacc cttactcact aaattaggtt aaagaatcaa cgatgtcaat cagggcgatg
    10501 caggtgtatc gcccttacca ctcccagact ttcgacggtg taaccaccgc aggaagaggg
    10561 atatcccact cttcaacggg gagtttttcc accaactgac aatcatgcgc ataacccacc
    10621 ggttgcgttt tatagtgctg ccagttttgt aaggtccgat cataaaaacc accgcccatt
    10681 cccaggcgct gaccgtactc atcaaaggcg accagcggtg tgatcagcac gtctaatcgg
    10741 gaaaggggta gcacgtcacg cacatccagt tttggctcat ggatcttcaa cctgttcatc
    10801 accagttcgc tttgcggatg gtaattcagg aacagcaaat taccggcact aaagggatgc
    10861 aaaactggaa gatatacgcg cttaccggcg cgccagagtt gttctattag cggctgggtg
    10921 tcgagttcac catcaaaaga gaggaataca gcgaccgtat gcgccatcac caccggggga
    10981 taagtcatca tccgggtagc ggcttgttga cccatttcca gctgttgttc cggcgttaac
    11041 gcacgacgac gttgccgaat cattttgcgg atttcttgtc gggataatgt cagtgggagc
    11101 tctggtagtt gcgtcatggc tggtgccaga taagaaggga atctccgaga tgccgccgca
    11161 ggctgtaacc cttgaaccct tggttcaagg tgaatgtgtc gtcgcagttt taaggcttct
    11221 cggacgaacc gagcatgctc accaaccgcg gagcgccaca ttcttgtggt atgaaatatc
    11281 ggctcagggg actggcccgc ttgcgaacat ctcagagaaa ttttgtcttc acggttactc
    11341 taccacagta aaccgaaaag tgtcattcaa agttttggtt agttttttcg gtgatgcgac
    11401 cttgttcaag taacgcttgt tctatggtct gctgcagcat ccgaatacgc tgttccatac
    11461 ttgccgcgta gtcacgagtc tttgcttttt cttgcgctaa ctcatagctg atattcaatg
    11521 cggcaatgaa gaccaactgt tcagtatttg tgactctagt gcgttctttc agatcttgca
    11581 accgttggtt cagatcgtcc gctgcctgat tcaacgcatc cctttggtca ggcgggcagt
    11641 tcacacgcag tgaacggcca aaaatttgga tatcgacggg ttgtgcagac atgccacctt
    11701 cctgctgatt gactgcgctg ccttcgtctt tagacccatg gtctgcgaag gggcgacgct
    11761 atagctaccc tgatgagaag agacaagccc ttttctggtc caccagggcc caaagtggta
    11821 gcatatcatg aatattcctc cctttgacga cgaatgctta tgtctataca gaacgaaatg
    11881 cctggttaca acgaaatgaa ccagtatctg aaccaacaag ggacgggtct gacccctgcc
    11941 gagatgcatg gtttaatcag tgggatgata tgtggcggta acgatgacag ctcatggctg
    12001 ccgctacttc acgacctgac gaacgaaggc atggctttcg gtcatgagct ggcacaggca
    12061 ctgcgtaaaa tgcactctgc caccagcgat gccctgcagg atgacggctt cctttttcag
    12121 ctttatctgc ctgatggcga tgatgtcagc gttttcgatc gggctgatgc actggcaggt
    12181 tgggtcaatc acttcctgct tggtcttggc gttacgcaac cgaagctgga caaagtgacc
    12241 ggcgaaaccg gtgaagccat cgacgatctg cgtaacatcg cgcagctggg ctacgacgaa
    12301 gacgaagatc aggaagagct tgaaatgtcg cttgaagaga tcatcgaata cgttcgtgtt
    12361 gccgcgctgt tatgccacga cacctttact catccgcaac cgaccgcgcc agaagtacaa
    12421 aaaccgactc tacactaaaa acaaaaaacg taaggagagt gttatgagtg agatatcccg
    12481 gcaagagttt cagcgtcgcc gtcaggccct ggtggagcaa atgcaacccg gcagcgccgc
    12541 gctgattttt gctgcaccag aagtaacacg tagcgccgac agcgaatacc cctatcgtca
    12601 gaacagtgac ttctggtact tcaccggctt taacgaaccg gaagcggtgc tggtgctgat
    12661 taaaagcgat gacactcata accacagcgt tctgtttaac cgcgttcgcg acctgacggc
    12721 ggagatctgg tttggccgtc gcttaggcca ggatgccgcg ccagagaaac tgggcgttga
    12781 ccgcgcactg gcattcagcg aaatcaatca gcaactttat caactactta acggcctgga
    12841 tgtggtttac catgcccggg gcgaatatgc atatgctgat gaaatcgtga acagtgcgct
    12901 ggaaaaactg cgtaaaggtt cgcggcaaaa tctcaccgca ccggcaacga tgatcgactg
    12961 gcgtcctgtt gttcatgaaa tgcgcctgtt caaatcgcca gaagagattg ccgtactccg
    13021 ccgcgcggga gaaatcaccg ccatggcaca tacacgggcg atggaaaaat gccgtccggg
    13081 aatgttcgag taccatctgg aaggcgaaat tcaccacgaa tttaaccgcc acggtgcgcg
    13141 ctatccttcc tataacacca ttgtaggcag cggtgaaaac ggctgcattc tgcactacac
    13201 cgaaaacgag agtgaactgc gcgacggtga cctggtgttg attgacgcgg gttgtgaata
    13261 caaaggttac gcgggcgata ttacccgcac cttcccggtc aacggcaaat tcacccaggc
    13321 ccagcgtgaa atctacgaca ttgtgctgga gtctctcgaa accagcctgc gcctgtatcg
    13381 tccgggaact tccattctgg aagtcactgg tgaagtggtg cgcatcatgg ttagcggcct
    13441 ggtaaaactc ggcatcctga aaggtaatgt tgatgaactg atcgctcaga acgcccatcg
    13501 tcctttcttt atgcatggcc ttagccactg gttaggactg gatgtccatg acgttggcgt
    13561 ttatggtcag gatcgctcgc gcattctgga accggggatg gtactgaccg tagagcctgg
    13621 tctgtatatc gcgccggatg cggatgtgcc agaacaatat cgcggtatcg gcattcgtat
    13681 tgaagacgac attgtgatta ccgaaaccgg caacgaaaac ctcaccgcca gcgtggtgaa
    13741 aaagccggaa gaaatcgaag cgttgatggc tgctgcgaga aagcaatgag cgtaatcatc
    13801 gtcggtggcg gcatggcggg cgcgacgctg gcgctggcta tttcccggtt aagtcacggg
    13861 gcgctgccgg tacatttgat tgaagcgact gcgccagagt cacatgctca tccgggcttt
    13921 gatggacgag cgatagcgct ggcggcgggt acctgtcagc aactggcgcg catcggcgtc
    13981 tggcaatctc tggctgattg cgcaaccgcc atcactaccg tgcatgtcag cgatcgtggt
    14041 catgctggat tcgtcaccct cgccgcagaa gattaccaac tggcggcgct ggggcaggtt
    14101 gtcgaattgc ataatgttgg gcaacggctg tttgcattgc tgcgaaaagc acctggcgta
    14161 atgctgcatt gccccgatcg cgtagctaac gttgcccgta ctcagagtca cgttgaagtg
    14221 acgctggaga gcggcgagat gctgacgggc cgcgtgctga tagccgctga tggcacccat
    14281 tcagcgttag ccaccgcgtg cggcgttgat tggcagcagg agccttacga acaactggca
    14341 gtgattgcca acgttgctac ttctgttgcg cacgaaggcc gcgcttttga acgcttcacg
    14401 caacatggcc cgctggcgat gttgccgatg tctgacggac gctgttcgct ggtctggtgt
    14461 catccactgg aacggcgcga agaggtgctg tcgtggagtg acgagaagtt ttgccgtgaa
    14521 ctccagtctg cctttggctg gcgactgggg caaattaccc acgccggtaa acgcagtgct
    14581 tatccgctgg cattaacccg cgccgccaga ccgattaccc atcgcactgt gctggtgggc
    14641 aatgcggcgc aaactctgca cccgattgcc gggcaagggt ttaacctcgg tatgcgagat
    14701 gtcatgagcc ttgcggaaac cctgactcag gcgcatgagc gcggagaaga catcggtgat
    14761 tacggcatat tgtgccgcta tcagcagcgt cgacagagcg atcgcgaagc aaccatcggc
    14821 gtcacggaca gcctggtaca tctttttgcc aaccgttggg caccgctggt tgtcgggcgc
    14881 aacatcggac tgatgacgat ggaattattc accccggcac gcgatgtgct ggcgcagcgc
    14941 accctcggtt gggtggcgcg ttgaaggcgg ttttaaggaa taaacaatgc aaagtgttga
    15001 tgtagccatt gttggcggcg gcatggtggg gctggcggtt gcctgcggct tacaggggag
    15061 cggcttacgc gttgccgtac tggagcagcg cgtaccggaa cctctggcgg tagatgcact
    15121 accacaactg cgcgtttcgg ctatcaatgc cgccagcgaa aaattactca cccgtcttgg
    15181 cgtctggcag gacattctcg ctcgtagggc cagttgctat cacggcatgg aagtgtggga
    15241 caaagacagt tttggtcaca tctcgtttga cgatcaaagc atgggctata gccatcttgg
    15301 gcacatcgtt gaaaactcag tgattcacta cgcgctgtgg aacaaagcgc agcagtcgtc
    15361 agatattact ctgttggccc ccacagaatt acagcaggtc gcctggggag aaaatgaaac
    15421 cttcctgacg ctgaaagatg gcagcatgtt aacggcgcgt ctggtgattg gcgcggacgg
    15481 tgctaattcc tggttgcgca acaaagcaga tattccgctg actttctggg attatcagca
    15541 tcacgcgctg gtagcgacca ttcgcacgga agaaccgcat gatgcggtgg cgcggcaggt
    15601 tttccatggc gaaggcattc tggccttttt accgcttagc gatccgcatc tttgctcgat
    15661 tgtctggtca ctgtcgccag aggaagcgca gcggatgcag caggcaagtg aagacgaatt
    15721 taatcgcgcg ttaaatatcg cttttgataa tcgccttggc ttatgcaagg ttgagagcgc
    15781 gcgtcaggtg ttcccgctga cggggcgtta tgcgcgccag tttgccgcac accgtctggc
    15841 gctggtgggc gacgccgcgc ataccattca cccactggcg gggcagggcg ttaatctcgg
    15901 atttatggac gccgcagagc tgattgctga actgaaacgg ttgcatcgtc agggtaaaga
    15961 catcgggcag tacatttacc tgcgccgcta tgaacgtagc cgcaagcaca gcgcggcgct
    16021 gatgctggct ggtatgcagg gttttcgcga tctcttttcc ggcgctaatc cggcgaaaaa
    16081 actgctgcgt gatattggtt tgaaactggc cgacacgctt cctggcgtta agccgcaact
    16141 tatccgccag gcaatgggat taaacgattt gtctgaatgg ctgcgttaaa aatttctcct
    16201 ctgttgttta tttgataccc atcacacttt catctcccgg ttttttcgcc gggagatttt
    16261 cctcatttga aataaactaa tttcacctcc gttttcgcat tatattttct aatgccatga
    16321 ttttttgatt tagtgttttt tgacattttt ttagctctta atattgtctt attcaaattg
    16381 actttctcat cacatcatct ttgtatagaa attggtgtat tttttggttt tttattctgt
    16441 cgcgattttt gcatttttta accataagct aatgtgatga tcaattttac cttatggtta
    16501 acagtctgtt tcggtggtaa gttcaggcaa aagagaacga ttgcgttggg gaccgggagt
    16561 ggctccgatg ctgggttttg tggtgataat ttcaccatga aaaagttgtc agccccgctt
    16621 attcaatgag gacaagatgg cacaacagac tcctttgtac gaacaacaca cgctttgcgg
    16681 cgctcgcatg gtggatttcc acggctggat gatgccgctg cattacggtt cgcaaatcga
    16741 cgaacatcat gcggtacgta ccgatgccgg aatgtttgat gtgtcgcata tgaccatcgt
    16801 cgatctccgc ggcagccgca cccgggagtt tctgcgttat ctgctggcga acgatgtggc
    16861 gaagctcacc aaaagcggca aagcccttta ctcggggatg ttgaatgcct ctggcggtgt
    16921 gatagatgat ctcatcgtct actactttac tgaagatttc ttccgcctcg ttgttaactc
    16981 cgccacccgc gaaaaagacc tctcctggat tacccaacac gctgaacctt tcggcatcga
    17041 aattaccgtt cgtgatgacc tttccatgat tgccgtgcaa gggccgaatg ctcaggcaaa
    17101 agctgccaca ctgtttaatg atgctcagcg tcaggcggtg gaagggatga aaccgttctt
    17161 tggcgtgcag gtgggcgatc tgtttattgc caccactggt tataccggtg aagcgggcta
    17221 tgaaattgcg ctgcccaatg aaaaagcggc cgatttctgg cgtgcgctgg tggaagcggg
    17281 cgttaagccc tgcggtctgg gggcgcgtga cacgctgcgt ctggaagcgg gcatgaatct
    17341 ttatggtcag gagatggacg aaactatttc tcctttagcc gccaacatgg gctggactat
    17401 cgcctgggaa ccgacagatc gtgactttat cggtcgtgaa gccctggaag tgcagcgtga
    17461 gcatggtaca gaaaaactgg ttggtctggt gatgaccgaa aaaggcgtgc tgcgtaatga
    17521 actgccggta cgctttaccg atgcgcaggg caaccagcat gaaggcatta tcaccagcgg
    17581 tactttctcc ccgacgctgg gttacagcat tgcgctggcg cgcgtgccgg aaggtattgg
    17641 cgaaacggcg attgtgcaaa ttcgcaaccg tgaaatgccg gttaaagtga caaaacctgt
    17701 ttttgtgcgt aacggcaaag ccgtcgcgtg atttactttt ttggagattg attgatgagc
    17761 aacgtaccag cagaactgaa atacagcaaa gaacacgaat ggctgcgtaa agaagccgac
    17821 ggcacttaca ccgttggtat taccgaacat gctcaggagc tgttaggcga tatggtgttt
    17881 gttgacctgc cggaagtggg cgcaacggtt agcgcgggcg atgactgcgc ggttgccgaa
    17941 tcggtaaaag cggcgtcaga catttatgcg ccagtaagcg gtgaaatcgt ggcggtaaac
    18001 gacgcactga gcgattcccc ggaactggtg aacagcgaac cgtatgcagg cggctggatc
    18061 tttaaaatca aagctagcga tgaaagcgaa ctggaatcac tgctggatgc gaccgcatac
    18121 gaagcattgt tagaagatga gtaacggctt tattcctctt ctgcgggaga ggatcagggt
    18181 gaggaaaatt tatgcctcac cctcactctc ttcgtaagga gagaggttca caattcactg
    18241 cacgtttcag gaaccatcgc tcatgacaca gacgttaagc cagcttgaaa acagcggcgc
    18301 ttttattgaa cgccatatcg gaccggacgc cgcgcaacag caagaaatgc tgaatgccgt
    18361 tggtgcacaa tcgttaaacg cgctgaccgg ccagattgtg ccgaaagata ttcagcttgc
    18421 gacaccaccg caggttggcg caccggcgac cgaatacgcc gcactggcag aactcaaggc
    18481 tattgccagt cgcaataaac gcttcacgtc ttacatcggc atgggttaca ccgccgtgca
    18541 gctaccgccg gttatcctgc gtaacatgct ggaaaatccg ggctggtata ccgcgtacac
    18601 tccgtatcaa cctgaagtct cccagggccg ccttgaagca ctgctcaact tccagcaggt
    18661 aacgctggat ttgactggac tggatatggc ctctgcttct cttctggacg aggccaccgc
    18721 tgccgccgaa gcaatggcga tggcgaaacg cgtcagcaaa ctgaaaaatg ccaaccgctt
    18781 cttcgtggct tccaatgtgc atccgcaaac gctggatgtg gtccgtactc gtgccgaaac
    18841 ctttggtttt gaagtgattg tcgatgacgc gcaaaaagtg ctcgaccatc aggacgtctt
    18901 cggcgtgctg ttacagcagg taggcactac cggtgaaatt cacgactaca ctgcgcttat
    18961 tagcgaactg aaatcacgca aaattgtggt cagcgttgcc gccgatatta tggcgctggt
    19021 gctgttaact gcgccgggta aacagggcgc ggatattgtt tttggttcgg cgcaacgctt
    19081 cggcgtgccg atgggctacg gtggcccaca cgcggcattc tttgcggcga aagatgaata
    19141 caaacgctca atgccgggcc gtattatcgg tgtatcgaaa gatgcagctg gcaataccgc
    19201 gctgcgcatg gcgatgcaga ctcgcgagca acatattcgt cgtgagaaag cgaactccaa
    19261 catttgtact tcccaggtac tgctggcaaa catcgccagc ctgtatgccg tttatcacgg
    19321 cccggttggc ctgaaacgta tcgctaaccg cattcaccgt ctgaccgata ttctggcggc
    19381 gggcctgcaa caaaaaggtc tgaaactgcg ccatgcgcac tatttcgaca ccttgtgtgt
    19441 ggaagtggcc gacaaagcgg gcgtactgac gcgtgccgaa gcggctgaaa tcaacctgcg
    19501 tagcgatatc ctgaacgccg tggggatcac ccttgatgaa accaccacgc gtgaaaacgt
    19561 aatgcagctt ttcagtgtgc tgctgggcga taaccacggg ctggagatcg acacgctgga
    19621 caaagacgtg gctcacgaca gccgctctat ccagcctgcg atgctgcgcg acgacgaaat
    19681 cctcacccat ccggtgttta atcgctatca cagcgaaacc gaaatgatgc gctatatgca
    19741 ctcgctggag cgtaaagatc tggcgctgaa tcaggcgatg atcccgctgg gttcctgcac
    19801 catgaaactg aacgccgccg ccgagatgat cccaatcact tggacggaat ttgccgaact
    19861 gcacccgttt tgcccgccgg aacaggctga aggttatcag cagatgattg cgcagctggc
    19921 tgactggctg gtgaaactga ccggttacga cgccgtttgt atgcagccga actccggcgc
    19981 acagggcgaa tacgcgggcc tgctggcgat tcgtcattat catgaaagcc gcaacgaagg
    20041 gcatcgcgat atctgcctga tcccggcttc tgcgcacgga actaaccccg cttctgcaca
    20101 tatggcagga atgcaggtgg tggttgtggc gtgtgataaa aacggcaaca tcgatctgac
    20161 tgatctgcgc gcgaaagcgg aacaggcggg cgataacctc tcctgtatca tggtgactta
    20221 tccttctacc cacggcgtgt atgaagaaac gatccgtgaa gtgtgtgaag tcgtgcatca
    20281 gttcggcggt caggtttacc ttgatggcgc gaacatgaac gcccaggttg gcatcacctc
    20341 gccgggcttt attggcgcgg acgtttcgca cctcaacctg cataaaactt tctgcattcc
    20401 gcacggcggt ggtggtccgg gtatgggacc gatcggtgtg aaagcgcatc tggcaccgtt
    20461 tgtaccgggt catagcgtgg tgcaaatcga aggcatgtta acccgtcagg gcgcagtttc
    20521 tgcggcaccg ttcggtagcg cctctatcct gccaatcagc tggatgtaca tccgcatgat
    20581 gggcgcagaa gggctgaaaa aagcaagcca ggtggcaatc ctcaacgcca actatattgc
    20641 cagccgcctg caggatgcct tcccggtgct gtataccggt cgcgacggtc gcgtggcgca
    20701 cgaatgtatt ctcgatattc gcccgctgaa agaagaaatc ggcatcagcg agctggatat
    20761 tgccaagcgc ctgatcgact acggtttcca cgcgccgacg atgtcgttcc cggtggcggg
    20821 tacgctgatg gttgaaccga ctgaatctga aagcaaagtg gaactggatc gctttatcga
    20881 cgcgatgctg gctatccgcg cagaaattga ccaggtgaaa gccggtgtct ggccgctgga
    20941 agataacccg ctggtgaacg cgccgcacat tcagagcgaa ctggtcgccg agtgggcgca
    21001 tccgtacagc cgtgaagttg cggtattccc ggcaggcgtg gcagacaaat actggccgac
    21061 ggtgaaacgt ctggatgatg tttacggcga ccgtaacctg ttctgctcct gcgtaccgat
    21121 tagcgaatac cagtaattca ctgattcgag tattttctaa aggcgcttcg gcgccttttt
    21181 agtcagatga caaagtacaa aagtgctcag acagtcccct cgcccctttg gggagagggt
    21241 tagggtgagg ggaacaggcc ggcactggcg cgaatattta ccctcacccc gaccatctcc
    21301 ctgaaagggc gagggggaaa agcgtgccaa cattgaagat tgagccagtt tgttagcaat
    21361 ctcaaagata cgtcaacgaa ttaatttttc tcggaaaaac aaatggctat agcacttgtg
    21421 accggtggca gtcgcggtat cgggcgggca actgcattac tgttggcgca agaagggtat
    21481 acggtggcgg ttaattatca gcaaaacctc cacgcggcgc aggaagtgat gaacttaata
    21541 atgcaagccg gtggcaaagc attcgtgctc caggcggata tcagcgacga aaaccaggtc
    21601 gttgcgatgt ttacagcaat cgatcagcac gatgaaccgc tagcagcgct ggtcaataac
    21661 gccgggatct tgtttaccca gtgcaccgtt gaaaacctta ccgcagagcg aatcaaccga
    21721 gtactttcca ccaacgtgac gggatatttt ctctgctgcc gcgaggcggt aaaacgcatg
    21781 gcgcttaaaa atggtggcag tggcggcgct atcgtcaatg tctcttcggt ggcctcacgg
    21841 ttgggttcgc caggggaata tgttgattac gcggcatcga aaggggcgat tgatacgtta
    21901 accaccggac gatcgctgga agtcgccgcg caggggatcc gcgttaactg cgtgcggcca
    21961 gggtttattt ataccgaaat gcacgccagc ggcggcgagc ctggacgcgt cgatcgcgtt
    22021 aagtcgaaca tccccatgca gcgtggtgga caggcagaag aggtcgcgca ggccattgtc
    22081 tggctactaa gtgataaagc ctcttacgtc acgggaagtt ttatcgattt ggcgggcggg
    22141 aaataaaaca gggaagttgt ctgaccggat gcaacaagta ttgcatccgg tacttcatcg
    22201 acttaaagct tctcgccgtt gctggcaatc acctctttgt accagttaaa gctcttctta
    22261 cgtgaacgcg acatatcgcc agtaccgtcg tcatgtttat tcacatagat aaagccgtag
    22321 cgtttgctgt actgcccggt agtgaatgac acgcagtcga tgcagcccca cggtgtgtag
    22381 cccatcagat ccacaccatc gtaagtcact gcttttttca tctcttcaat atgggcgcgc
    22441 aggtagtcaa tgcggtagtc gtcgttgatg ctgccatctt cttccacttt gtcgtaagcg
    22501 ccaaaaccgt tttcgacaat aaacagcggc ctctgataac gctcatacag ttcgcaaagg
    22561 gcatagcgca gacctactgg atcaatctgc cagccccagt cagacgcttt aacatacggg
    22621 ttcggtacgc tgccttcaaa accagagatc gcatcgccgg tgccgccttc ggccttcact
    22681 gcgttggtca tgtaatagct gaaaccaaga taatcgcagg tgccttcgcg cagtacatcg
    22741 agatcgccgt cttccatttt gatattaaat ccgcgacgtt cccactcgtt caatacatag
    22801 gacgggtaat agccgcatag ctgcacatcg gtaaagacgt agcgttcgcg catcgactcc
    22861 tgagcgaaca tcacatcgtc cgggttacat gagtagggat agagcggcac catcgccagc
    22921 atacagccga ctttcatctc cgggttaatg cgacgcgcgg ctttcactgc cagggcgctg
    22981 gcgacaaact ggtgatgcag cacctggtac atcgtctctt ccgggttgtc atgctcggta
    23041 tacaccacgc cggagcagca gtaaccaaac agcggcgcac gccagttacg ctggttgttt
    23101 atttcgttga aggtcatcca gtatttgact ttatgcttgt agcgttcgaa taccacttcc
    23161 gcgaaacgga caaagaaatc aacgaattta cggttggtcc agctaccgta ttgctgcacc
    23221 agatgcagcg gcatttcaaa gtgggagagg gttatcaccg gttcgatgtt gtatttcagc
    23281 agttcatcga acagagagtc gtaaaacttc agcccttctt cgtttggctg agcttcatca
    23341 ccttttggaa aaatacgcgt ccaggcgatg gaagtccgaa aacatttgaa gcccatttcg
    23401 gcaaatagct tgatgtcttc cttgtagtga ccataaaaat caacggcttc atggtttgga
    23461 tagtattttc ctggcaagac ttctttggtg atttcgcgcg gcacgccgtg tgcgccaccg
    23521 gtcagaacgt cacaaatgct cggtcctttg ccgcctttgt tccagccgcc ttcgacctga
    23581 tgagcggcaa cggcgccacc ccataagaaa tctttcggta aggtgagttt tttcactatc
    23641 ataggctcca gatcagtcaa ttcttacagg aaatagtacc atgcagccaa acgacatcac
    23701 tttttttcaa cgtttccagg atgacattct ggctgggcgt aaaaccatca ccatccgcga
    23761 cgagtctgaa tcgcacttca aaacgggtga tgtgcttcgt gtcggacgtt ttgaagatga
    23821 cggttatttt tgcacgattg aagtcaccgc aacctcaacc gtaacgctgg atacactgac
    23881 ggaaaaacat gcagagcagg aaaatatgac cctgcctgaa ctgaaaaagg tcattgctga
    23941 catctatccc ggtcagacac aattttatat gattgaattt aaatgtcttt aaattgaact
    24001 cacgcctgtt agttgaaatt tcgcatttaa tttcatgatt tttaggagat tagtctcaaa
    24061 tcagaattta ttttagctaa caggtgttca ctggaactat tctcagttac gctggagagg
    24121 tatacatgtg cgtgtacgtt tctctggagt aagttatggt tcagaagccc ctcattaagc
    24181 agggatattc gctggcagag gaaattgcca acagcgtcag tcacggcatt gggctggtgt
    24241 ttggtatcgt tgggctggtg ttgctactgg ttcaggcggt ggatctcaat gccagcgcca
    24301 cagcgataac cagttacagc ctctatggcg gcagtatgat cctgctgttc ctcgcttcga
    24361 cgctctatca cgccattcct catcaacggg caaaaatgtg gctgaagaaa tttgaccatt
    24421 gcgctattta cctgttgatt gccggaacct acacgccgtt tttgctggtg gggctggatt
    24481 ctccgttagc gcgcgggttg atgattgtta tctggagcct agcattgctg gggattctgt
    24541 tcaaactgac catcgcgcac cgattcaaaa ttttatctct ggtgacttat ctggcgatgg
    24601 gctggctgtc gctggtggta atttatgaaa tggcagttaa gctcgcggcg ggcagcgtta
    24661 ccttactggc ggtaggcggc gtggtttatt cgctcggggt gattttctac gtctgcaaac
    24721 gcattccata caaccatgcc atctggcacg gcttcgtgct cggcggtagt gtgtgccact
    24781 ttctggcgat ctatttgtat attgggcagg cgtaatgggt atgcctttgt aggcctgata
    24841 agacgcgtca gcgtcgcatc aggcatcgga gcgcaaccgc cggatgcggc gtgaacgcct
    24901 tatccgatct acgggcctcg tccctgttgt gataaaggca tcaaatccca gacatgataa
    24961 gacgcgtcag cgtcgcatca ggcgataaat gcctgatgcg atacagagat ttactcttcg
    25021 agcgaatacg gcagcggctc gatatgcagt gtattcgcat cgtcgcgtac gcggaagatg
    25081 ctgtccggtt ccatatcgtt attcatgacg acctgtacca cgacctgacc atcttccagt
    25141 tttaccgcag ccagcaccgt accggtacga cgccagttct cgcccatttt cagctctaag
    25201 tcttcaccag cttccggcag tcggctggca ctacctgcca gcaaccagag cgcgcgtttg
    25261 ttggcaccac ggaattttgc tcgcgccacc atctcttgtc cggtataaca gcctttctta
    25321 aagctgatac cgcccagcgc ctggagattg gtcgcctgtg ggataaactg cccgctgttg
    25381 gcggcatcaa tcaccgggaa accagcttca atgtttaatg ccagccactg ttggctattg
    25441 ttcagttccg cttcaccgcg cagtttatcg gtcagcatat tagcagtagc ttcatcggtt
    25501 acgatcagga aacgttctgc cgggtgttca aaccatagca aagtggtcgc gccttctttg
    25561 actacctgtt tttctttcga aggcagttcg ctaaagagat ttgccagcgc ggcgcgcgcc
    25621 tgaaaaccgg caacaccaag cagcacacgc tcgtcgtctg gcgcgatggt cactttagag
    25681 aataccgcat attttttcag ttcagtcagc tgcggttcac gcacgctgcg ccgttcaatc
    25741 catgcaaagc catcgccgtc gcggaacaga cgtaaattgc tccacatttt acctttggcg
    25801 tcgcaatggg cggcgagcag gtgctgatct tctgccatct ggctgacatc tgctgtcacc
    25861 tgaccctgca tatatttttc gctgtccgcg ccagtaatgg tggcaagcgc ccagtcatca
    25921 agcgtcatca gcgtcagcgg taaacgggca gaagccgtag gctgacgtgg aggaaaaggt
    25981 gtaaaagcca taatagagtc ctgagttgct taacggcgta ttaatgacta atggtaaaag
    26041 agctatgagg caatgcaagt gctttcacta cgcgatctgt gccttctgcg aggcgactgc
    26101 caaaaaaatg aagcccttga attgttgtga attaatttcg cgatcgcgat ggtgtttact
    26161 gatattcccg taaaagaagt cgaaaaacac gttacaatag ccgggtactg gattttcgtg
    26221 agacaggaag aagatggaca ttaacaacaa agcccgcatt cattgggcat gccgccgtgg
    26281 tatgcgcgaa ctcgatattt caatcatgcc gtttttcgaa catgagtacg acagcttaag
    26341 cgatgacgaa aaacgcatct ttattcgtct gctggaatgt gacgatccgg acctgtttaa
    26401 ctggctgatg aatcacggta aaccggccga tgcagaactg gaaataatgg tccgactcat
    26461 ccagacacgg aaccgggaac gtggtcctgt ggcaatctga tttgcgcgtc tcctggcgcg
    26521 cacagtggct ttccttgctg attcatgggc tggttgccgc tgttatttta ctcatgccct
    26581 ggccgctcag ttacaccccg ttatggatgg tgttactttc gctggtggta tttgattgcg
    26641 ttcgcagcca gcggtgtatt aatgctcgcc agggggaaat tcgcttgttg atggacgggc
    26701 gtttgcgttg gcaagggcag gagtggagca tcgtcaaagc accgtggatg attaagagcg
    26761 gcatgatgct gcgtttacgt tctgatggcg gtaaacggca acatttatgg ctggcagccg
    26821 acagcatgga cgaagccgaa tggcgggatt tacggcggat tttgttgcaa caagagacgc
    26881 aaagataaag atgacggcaa agcatatgcg ctaagccgtc agtgagatca ggcgtaatgc
    26941 tctgccattt cgttgaggat ttgctcgcac cagctctgaa tacgctcgtc gctaaggtca
    27001 tactggttag tttcatccag cgccagaccc acgaacagtt gcccgtcagc aatcaccggt
    27061 ttcgggctgg taaattcata tccttccgtt ggccagtagc cgacgaactt cacgcctttg
    27121 gtcgagagtt tgtcatgcag cataccgagc gcatcgagga accactcgcc gtatcccagt
    27181 tgatcgccaa gcccatacag cgcaataatt ttaccttcaa ggttcaggtc gtcgagctga
    27241 tcccagacgg cttcccagtc ttcttggatt tcaccaaaat cccaggtcgg gatacccaga
    27301 atgagcacat cgtactgctc cattaatttc ggggagtcgt ccttgaggtt atgtaaggtc
    27361 accagttctg ggccgataat atcgcggatt ttttctgccg ccatttcggt gtaacaggtg
    27421 ctggaaccgt aaaaaagacc catattcata acgtaagctt ctcgattctg ctgtggcttt
    27481 tgcagctagt gtatcagaat cgctttagtc acggcacaat aaggcataat ggatcaaatg
    27541 agtgaccata aggggcgcaa gtgaaacagg atctggcacg catcgagcag tttcttgatg
    27601 ctctgtggct ggaaaaaaat ctggctgaaa atacgttgaa cgcttaccgt cgcgatctgt
    27661 taatgatggt ggagtggttg catcaccgcg ggttgacgct ggcgacggcg caaagtgacg
    27721 atttgcaggc attactggca gaacggctgg agggcgggta taaagccacc agctcagcgc
    27781 gtttgctgag tgcagtgcgc cgattgttcc agtatcttta tcgcgaaaag tttcgtgaag
    27841 acgatcccag tgcgcatctc gcttcaccga aattgcccca gcgtttgcca aaagatttaa
    27901 gcgaagcgca ggtcgaacgt ttattacagg cgccattaat tgatcagcca ctggagctac
    27961 gcgataaagc catgcttgaa gtgttgtatg ctaccgggct gcgtgtctct gaactggtcg
    28021 ggctgacaat gagtgatatc agcctgcgtc agggcgtagt gcgggtcatt ggtaaaggca
    28081 acaaagagcg cctggtgccg ttaggggaag aggcggttta ctggctggaa acctatctgg
    28141 aacatgggcg tccgtggctg ctgaatggtg tgtcaattga cgtgttgttt cccagccagc
    28201 gtgcgcagca gatgacgcga cagaccttct ggcaccgcat taaacattat gccgtgctgg
    28261 cgggtatcga cagcgaaaag ctgtcaccgc atgtgttgcg tcacgctttt gccactcatt
    28321 tattaaatca tggtgcggat ttacgcgtgg tgcagatgct actgggccac agcgatcttt
    28381 ccaccacgca aatttatacg catgtcgcta ccgagcgtct gcggcaactt catcaacagc
    28441 atcacccgcg ggcgtgatgt ctgaaaagaa cgggaagatt tatgaagaaa ggttttatgt
    28501 tgtttacttt gttagcggcg ttttcaggct ttgctcaggc tgatgacgcg gcaattcaac
    28561 aaacgttagc caaaatgggc atcaaaagca gcgatattca gcccgcgccc gtagctggca
    28621 tgaagacagt tctgactaac agcggtgtgt tgtacatcac cgatgatggt aaacatatca
    28681 ttcaggggcc aatgtatgac gttagtggca cggctccggt caatgtcacc aataagatgc
    28741 tgttaaagca gttgaatgcg cttgaaaaag agatgatcgt ttataaagcg ccgcaggaaa
    28801 aacacgtcat caccgtgttt actgatatta cctgtggtta ctgccacaaa ctgcatgagc
    28861 aaatggcaga ctataacgcg ctggggatca ccgtgcgtta tcttgctttc ccgcgccagg
    28921 ggctggacag cgatgcagag aaagaaatga aagctatctg gtgtgcgaaa gataaaaaca
    28981 aagcgtttga tgatgtgatg gcaggtaaaa gcgtcgcacc agccagctgc gacgtggata
    29041 ttgccgacca ttacgcactt ggcgtccagc ttggcgttag cggtactccg gcagttgtgc
    29101 tgagcaatgg cacacttgtt ccgggttacc agccgccgaa agacatgaaa gaattcctcg
    29161 acgaacacca aaaaatgacc agcggtaaat aattcgcgtg aaacaacaga tacaacttcg
    29221 tcgccgtgaa gtcgatgaaa cggcagactt gcccgctgaa ttgcctccct tgctgcgccg
    29281 tttatacgcc agccggggag tacgcagtgc gcaagaactg gaacgcagtg ttaaaggtat
    29341 gttgccctgg cagcaactaa gcggtgtcga aaaggccgtt gagatccttt acaacgcttt
    29401 tcgcgaagga acgcggatta ttgtggtcgg tgatttcgac gccgacggcg cgaccagcac
    29461 ggctctaagc gtgctggcga tgcgctcgct tggttgcagc aatatcgact acctggtacc
    29521 aaaccgtttc gaagacggtt acggcttaag cccggaagtg gtcgatcagg cccatgcccg
    29581 tggcgcgcag ttaattgtca cggtggataa cggtatttcc tcccatgcgg gagttgagca
    29641 cgctcgctcg ttgggcatcc cggttattgt taccgatcac catttgccgg gcgacacatt
    29701 acccgcagcg gaagcgatca ttaaccctaa cttgcgcgac tgtaatttcc cgtcgaaatc
    29761 actggcaggc gtgggtgtgg cgttttatct gatgctggcg ctgcgcacct ttttgcgcga
    29821 tcagggctgg tttgatgagc gcggcatcgc aattcctaac ctggcagaac tgctggatct
    29881 ggtagcgctg gggacagtag cggacgtcgt gccgctggac gctaataatc gcattctgac
    29941 ctggcagggg atgagtcgca tccgtgccgg aaagtgccgt ccagggatta aagcgctgct
    30001 ggaagtggca aatcgcgatg cacaaaaact agccgccagc gatttaggtt ttgcgctggg
    30061 accgcgtctc aatgctgccg ggcgactgga tgatatgtcc gttggtgtgg cgctgttgct
    30121 gtgcgacaac atcggagaag cgcgcgtgct ggcaaatgaa ctcgatgcgc taaaccagac
    30181 gcgaaaagag atcgaacaag gaatgcaggt tgaagccctg accctgtgcg agaaactgga
    30241 gcgcagccgt gacacgctac ccggcgggct ggcaatgtat caccccgaat ggcatcaggg
    30301 cgttgtcggt attctggctt cgcgtatcaa agagcgtttt caccgtccgg ttatcgcctt
    30361 tgcgccagca ggtgacggta cgctgaaagg ttccggtcgc tccattcagg ggctgcatat
    30421 gcgtgatgcg ctggagcgat tagacacact ctaccccggc atgatgctca agtttggcgg
    30481 tcatgcgatg gcggcgggtt tgtcgctgga agaggataaa ttcgaactct ttcaacaacg
    30541 gtttggcgaa ctggttactg agtggctggc cccttcgcta ttgcaaggcg aagtggtgtc
    30601 agacggtccg ttaagcccgg ccgaaatgac catggaagtg gcgcagctgc tgcgcgatgc
    30661 tggcccgtgg gggcagatgt tcccggagcc gctgtttgac ggtcatttcc gtctgctgca
    30721 acagcggctg gtgggcgaac gtcatttgaa ggtgatggtc gaaccggtcg gcggcggtcc
    30781 actgctggat ggtattgctt ttaatgtcga taccgctctc tggccggata acggcgtgcg
    30841 cgaagtgcaa ctggcttata agctcgatat caacgagttt cgcggcaacc gcagcctgca
    30901 aattatcatc gacaatatct ggccaattta gcgtcatctt ctctataaaa aagagcgtgg
    30961 attgggtaca atcccgctct tatcaccgca ttttgactag ctcaataaaa gaaatcagac
    31021 catgtttgaa attaatccgg taaataatcg cattcaggac ctcacggaac gctccgacgt
    31081 tcttaggggg tatctttgac tacgacgcca agaaagagcg tctggaagaa gtaaacgccg
    31141 agctggaaca gccggatgtc tggaacgaac ccgaacgcgc acaggcgctg ggtaaagagc
    31201 gttcctccct cgaagccgtt gtcgacaccc tcgaccaaat gaaacagggg ctggaagatg
    31261 tttctggtct gctggaactg gctgtagaag ctgacgacga agaaaccttt aacgaagccg
    31321 ttgctgaact cgacgccctg gaagaaaaac tggcgcagct tgagttccgc cgtatgttct
    31381 ctggcgaata tgacagcgcc gactgttacc tcgatattca ggcggggtct ggcggtacgg
    31441 aagcacagga ctgggcgagc atgcttgagc gtatgtatct gcgctgggca gaatcgcgtg
    31501 gtttcaaaac tgaaatcatc gaagagtcgg aaggtgaagt ggcgggtatt aaatccgtga
    31561 cgatcaaaat ctccggcgat tacgcttacg gctggctgcg tacagaaact ggcgttcacc
    31621 gcctggtgcg taaaagcccg tttgactccg gcggtcgtcg ccacacgtcg ttcagctctg
    31681 cgtttgttta tccggaagtt gatgatgata ttgatatcga aatcaacccg gcggatctgc
    31741 gcattgacgt ttatcgcgcg tccggcgcgg gcggtcagca cgttaaccgt accgaatctg
    31801 cggtgcgtat tacccacatc ccgaccggga tcgtgaccca gtgccagaac gaccgttccc
    31861 agcacaagaa caaagatcag gccatgaagc agatgaaagc gaagctttat gaactggaga
    31921 tgcagaagaa aaatgccgag aaacaggcga tggaagataa caaatctgac atcggctggg
    31981 gcagccagat tcgttcttat gtccttgatg actcccgcat taaagatctg cgcaccgggg
    32041 tagaaacccg caacacgcag gccgtgctgg acggcagcct ggatcaattt atcgaagcaa
    32101 gtttgaaagc agggttatga ggaaccaaca tgtctgaaca acacgcacag ggcgctgacg
    32161 cggtagtcga tcttaacaat gaactgaaaa cgcgtcgtga gaagctggcg aacctgcgtg
    32221 agcagggaat tgccttcccg aacgatttcc gtcgcgatca tacctctgac caattgcacg
    32281 cagaattcga cggtaaagag aacgaagaac tggaagcgct gaacatcgaa gtcgccgttg
    32341 ctggccgcat gatgacccgc cgtattatgg gtaaagcgtc tttcgttacc ctgcaggatg
    32401 tgggtggccg cattcagctg tacgttgccc gtgacgatct gccggaaggc gtttacaacg
    32461 agcagttcaa aaaatgggac ctcggcgaca tcctcggcgc gaaaggtaaa ctgttcaaaa
    32521 ccaaaaccgg cgaactgtct atccactgta ccgagctgcg tctgctgacc aaagcactgc
    32581 gtccgctgcc ggataaattc cacggcttgc aggatcagga agcgcgctat cgtcagcgtt
    32641 atctggatct catctctaac gatgaatccc gtaacacctt taaagtacgc tcgcagatcc
    32701 tctctggtat tcgccagttc atggtgaacc gtggctttat ggaagttgaa acgccgatga
    32761 tgcaggtgat cccaggcggt gccgctgcgc gtccgtttat cacccaccat aacgcgctgg
    32821 atctcgacat gtacctgcgt atcgcgccgg aactgtacct caagcgtctg gtggtcggtg
    32881 gcttcgagcg tgtattcgaa atcaaccgta acttccgtaa cgaaggtatt tccgtacgtc
    32941 ataacccaga gttcaccatg atggaactct acatggctta cgcagattac aaagatctga
    33001 tcgagctgac cgaatcgctg ttccgtactc tggcacagga tattctcggt aagacggaag
    33061 tgacctacgg cgacgtgacg ctggacttcg gtaaaccgtt cgaaaaactg accatgcgtg
    33121 aagcgatcaa gaaatatcgc ccggaaaccg acatggcgga tctggataac ttcgactctg
    33181 cgaaagcgat tgctgaatct atcggtatcc acgttgagaa gagctggggt ctgggccgta
    33241 tcgttaccga gatcttcgaa gaagtggcag aagcacatct gattcagccg accttcatta
    33301 ctgaatatcc ggcagaagtt tctccgctgg cgcgtcgtaa cgacattaac ccggaaatca
    33361 cagaccgctt tgagttcttc atcggtggtc gtgaaatcgg taacggcttt agcgagctga
    33421 atgacgcgga agatcaggcg caacgcttcc tggatcaggt tgccgcgaaa gacgcaggtg
    33481 acgacgaagc gatgttctac gatgaagatt acgttaccgc actggaacat ggcttaccgc
    33541 cgacagcagg tctggggatt ggcatcgacc gtatggtaat gctgttcacc aacagccata
    33601 ccatccgcga cgttattctg ttcccggcga tgcgtccggt gaaataagca ttacgttatg
    33661 ctcacaaccc cggcaaatgt cggggttttt tatttaagct gggtaaatgc agataatagt
    33721 attctggctt cgcgatttgt cgcctgcatc accatccacg gactgaacgc ccacggcgtg
    33781 gcatcaatac cgcgtaatac atcagctaaa tcacaccatt gataatccat cacttcatca
    33841 tcgttgatct gtaacgcact ggtggtgcgt gcggcaaata ccggacacac ttcattttcc
    33901 acaatgccac tcggatcggt ggcgcggtag cgaaagtcag gatagataga ttcaggaggc
    33961 gtaatttcca cgccaagctc ataacggcaa cggcggatca ccgcgtcttc gctgctttct
    34021 cccagttgtg ggtgcccaca aaccgagtta gtccacacgc caggccacgc ttttttgctc
    34081 agtgcgcggc gggtaactaa taattgcccc ttggcattaa acagccaact ggagaacgcg
    34141 agatgtaaga gggtgtctgc cgtgtgtgcg gcatactttt ccagcgtacc cgtgggaact
    34201 ccctgtgcat tcaataaaat gacgtgttcc gtttgcataa tttctcacat gtaattctga
    34261 tcactcatca ttatagataa ttgaagagtg aaatgattaa ccaagagagc gtttatcgac
    34321 aaccgcccgt tttgagcgga tgccgacagg taaaaaccat ttaaaaaagt agcatcatat
    34381 atttgaagcg actttcaatg gccattgcaa cacagtcaga aatgacagga atagagagag
    34441 taaaaagcaa caacagactg aatatagcgc gcattaaaaa attcatatta ttctccatgc
    34501 tcatttttct tcagcttgcg catcagaagc cacatacgta ctgtcctgac ttttaaatct
    34561 ttatcagaga cggattcctt caccaggtca gcatgatgct gatagccatt aaagaagacg
    34621 ttcagcacaa cggcgctaag ggttgcgagc ataatgccgc tatgtagcag cggttgcagt
    34681 acggccggta acttagaaaa gaaatcgtga gagagcgtcg gagtcatgcc aacgccgaga
    34741 ctgatcgcca caatatagag gttgtaacgg ttggtggtgt agttacagcg cgacagaatt
    34801 cgaatccctg tcgccagtac catgccgaac atcactagac cagcgccgcc cagcacaaat
    34861 tgcggaatgg aggctaccag caccgccatt tttggcacca tgccaaacag gattaatata
    34921 attcccgaag aaatacacac ccagcggcta tgaacgcgca tcacgctaac caggccaacg
    34981 ttttgagaaa atgacgtgtg ggggaagctg ttaaacgtgc cgcctatcat tgtccctacg
    35041 ccatcgacac gcagcccgcg aataatatcg tgtgaagaga gtttacgacc gactatttca
    35101 cccagtgcca ggaacatccc catcgactcg ataaacacga tgattaacac ggcagtcatg
    35161 gtcagaatgg aaacgggatc gaaaatcggc ataccaaacg acatcggggt gacaatcgca
    35221 aaccacgagg catcatgtag cccggataaa ttgacttcat tcatcatcca cgaaagtaaa
    35281 aagccaaata caatccccag taatacggcg acgttggaca taaatccttt cgcatagcga
    35341 gtaatgagca agataaaaat taagacggca aaggagatac ctaaataaac gggattacca
    35401 tattgcggat tccctttacc tccagcggcc cagtcaatac ccacctgaat gatgctaagc
    35461 ccaatagaag taataaccac accggtaacc agtggcggga ataaaggcat caagcgacca
    35521 ataagtggcg ctaataatgt ggtgataaaa cccgcggcga tagtggcacc aaatatcccc
    35581 agcaggccga tatccgggtt catcccaatg gctatcattg gtgttacagc agcaaaggtc
    35641 accgacataa tcaccggcag gcggatcccc ataaagcggc cgataccgat acattgcaat
    35701 aatgtgacga tcccgcagca aaagagatcc gagctaatga gcatcgcaat agcatctttt
    35761 gagaggccca gtcggtcacc aatcataaga ggaacagcga ctgcacctgc gtacatgaca
    35821 aggacgtgtt gcagaccgag tatgatcagc tttcctggtg ataatatgcg gtcaacctca
    35881 tcagttgggc ggtcttgccc agaagatgag ggaagttggg aatctatggc gctcatcagt
    35941 gtttctcctt tcgcccgcca gaataaaaag caaccgttta cgttaatgaa actgtctaaa
    36001 cacatgctcg tggagtgcca gtttttgcga ggaaggggag ttagagacag gaattgcgga
    36061 ggcaatcaca taatcgtgct taaaccacga aaatgacaag agaacctgat aatggaatta
    36121 tctgtaattt tctcatattg atatttcatt aatcattctg gc
//
LOCUS       DADMEQ010000027        35963 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.027, whole genome shotgun sequence.
ACCESSION   DADMEQ010000027 DADMEQ010000000
VERSION     DADMEQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.027"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(269..886)
                     /gene="tdk"
                     /locus_tag="JNZ05_001404"
     CDS             complement(269..886)
                     /gene="tdk"
                     /locus_tag="JNZ05_001404"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAY8564689.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQT
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1491..1904
                     /gene="hns"
                     /locus_tag="JNZ05_001405"
     CDS             1491..1904
                     /gene="hns"
                     /locus_tag="JNZ05_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HAY8564690.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2048..2956)
                     /gene="galU"
                     /locus_tag="JNZ05_001406"
     CDS             complement(2048..2956)
                     /gene="galU"
                     /locus_tag="JNZ05_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAY8564691.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="JNZ05_001407"
     CDS             complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="JNZ05_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HAY8564692.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFHDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="JNZ05_001408"
     CDS             complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="JNZ05_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HAY8564693.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIIPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5281..5739
                     /locus_tag="JNZ05_001409"
     CDS             5281..5739
                     /locus_tag="JNZ05_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HAY8564694.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLSVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5789..6631
                     /gene="purU"
                     /locus_tag="JNZ05_001410"
     CDS             5789..6631
                     /gene="purU"
                     /locus_tag="JNZ05_001410"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HAY8564695.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6791..6875
                     /locus_tag="JNZ05_001411"
     tRNA            6791..6875
                     /locus_tag="JNZ05_001411"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6825..6827,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="JNZ05_001412"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="JNZ05_001412"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="JNZ05_001413"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="JNZ05_001413"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAY8564696.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="JNZ05_001414"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="JNZ05_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAY8564697.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="JNZ05_001415"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="JNZ05_001415"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY8564698.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="JNZ05_001416"
     CDS             complement(10156..13899)
                     /locus_tag="JNZ05_001416"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAY8564699.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="JNZ05_001417"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="JNZ05_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HAY8564700.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="JNZ05_001418"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="JNZ05_001418"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HAY8564701.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="JNZ05_001419"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="JNZ05_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HAY8564702.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="JNZ05_001420"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="JNZ05_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HAY8564703.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNFGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQMRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="JNZ05_001421"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="JNZ05_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HAY8564704.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /locus_tag="JNZ05_001422"
     CDS             complement(20438..21133)
                     /locus_tag="JNZ05_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY8564705.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="JNZ05_001423"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="JNZ05_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HAY8564706.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="JNZ05_001424"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="JNZ05_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HAY8564707.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGFLSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23296..23403
                     /locus_tag="JNZ05_001425"
     CDS             23296..23403
                     /locus_tag="JNZ05_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrA/LdrC"
                     /protein_id="HAY8564708.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(23552..24406)
                     /gene="kdsA"
                     /locus_tag="JNZ05_001426"
     CDS             complement(23552..24406)
                     /gene="kdsA"
                     /locus_tag="JNZ05_001426"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HAY8564709.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24442..25251)
                     /locus_tag="JNZ05_001427"
     CDS             complement(24442..25251)
                     /locus_tag="JNZ05_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HAY8564710.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25255..25647)
                     /locus_tag="JNZ05_001428"
     CDS             complement(25255..25647)
                     /locus_tag="JNZ05_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HAY8564711.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25644..26477)
                     /gene="prmC"
                     /locus_tag="JNZ05_001429"
     CDS             complement(25644..26477)
                     /gene="prmC"
                     /locus_tag="JNZ05_001429"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY8564712.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26477..27559)
                     /gene="prfA"
                     /locus_tag="JNZ05_001430"
     CDS             complement(26477..27559)
                     /gene="prfA"
                     /locus_tag="JNZ05_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAY8564713.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27601..28857)
                     /gene="hemA"
                     /locus_tag="JNZ05_001431"
     CDS             complement(27601..28857)
                     /gene="hemA"
                     /locus_tag="JNZ05_001431"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HAY8564714.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKNLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29071..29694
                     /gene="lolB"
                     /locus_tag="JNZ05_001432"
     CDS             29071..29694
                     /gene="lolB"
                     /locus_tag="JNZ05_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HAY8564715.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29694..30545
                     /gene="ispE"
                     /locus_tag="JNZ05_001433"
     CDS             29694..30545
                     /gene="ispE"
                     /locus_tag="JNZ05_001433"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAY8564716.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLKYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30696..31643
                     /locus_tag="JNZ05_001434"
     CDS             30696..31643
                     /locus_tag="JNZ05_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAY8564717.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31768..33447
                     /gene="dauA"
                     /locus_tag="JNZ05_001435"
     CDS             31768..33447
                     /gene="dauA"
                     /locus_tag="JNZ05_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HAY8564718.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPASVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33502..33780)
                     /gene="ychH"
                     /locus_tag="JNZ05_001436"
     CDS             complement(33502..33780)
                     /gene="ychH"
                     /locus_tag="JNZ05_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HAY8564719.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34058..34642
                     /gene="pth"
                     /locus_tag="JNZ05_001437"
     CDS             34058..34642
                     /gene="pth"
                     /locus_tag="JNZ05_001437"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY8564720.1"
                     /translation="MTIKLIVGLENPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34759..35850
                     /gene="ychF"
                     /locus_tag="JNZ05_001438"
     CDS             34759..35850
                     /gene="ychF"
                     /locus_tag="JNZ05_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAY8564721.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT         8847 a         9373 c         8826 g         8917 t
ORIGIN      
        1 ttctggggcc gtttttttgt ttacccaaag caacttttcc ataaaccgac agcattagcc
       61 ttcatcatat ttgcgacgat gtataacgcc taaacacagg gatattgtac tttacaggtc
      121 acaagtcaac gtcggtgctt aagagccctg tgaggcgtat agcggcgtta aaaaactgcc
      181 gagaagggta tatagcccgg aagaagtgcg taaaacgaac tgacaggata aaagtgccct
      241 gctcaccctg tcagtaaaga aattcttatt aatcgtggcg atgcctttcc tgaatagccg
      301 ttaatgagcc gacttgtaac gcctctttat agtgtttacg gcatacagaa acgtatcgtt
      361 cattaccacc aattaccacc tgctcacctt cgttataagg tctgcctgtt tgatcaagac
      421 gcagcaccat gcttgcttta cggccacaaa aacagatggt ttttaattca accagtttgt
      481 cggaccatgc cagtaagtat tggctgccaa taaataattc acctcgaaaa tcggtacgta
      541 aaccataaca aagtacgggt atatcgagtt gatcgacaac ctccgataat tcatatactt
      601 gttgtctggt taaaaactgg cattcatcaa ccagtacgca atgaattgcc tgctgttcat
      661 gttccgcacg aatctcatca aataatgatg aattttggtt aaataatttt gcaggcgatg
      721 acaaacctat acgcgaactg actttcccgg caccaaagcg atcatcaatt tctgccgtat
      781 atacgacagt gcgcatgccg cgttcctggt aattgtatga agattgcaac aatgctgtag
      841 acttacccgc attcattgcg gaatagtaga aatatagctg tgccatcagc cacaggccct
      901 caatgatatg aaataaagtt ggctggagtt tatcataatt cgtcagtttt tcagctcaac
      961 agtatgcaca gaagtgggta tatgcgttct cccttacgaa gccttgcata atccttctga
     1021 gctatcatta caactgcctc gcttgttata agcgggtaaa tgactgctgg taactattca
     1081 caatctttaa cctgttgcgc aagtaatagc cctctgttga cctccaggag atagtgcaat
     1141 actaagtcca tgctcttatt gcgactgttc tacttttcat cattcgctta atagggaatt
     1201 ctcgtaaaca caactaatac agaagactga aaggtcgtca gcctacgata atctccccat
     1261 aaaatgtgac atgaatcagg aagttttaac ctcacgtgct gcgaaatcat cggtgtaaat
     1321 agggctatat gccgcgtctt ttctggctaa ttttatgaaa agatatttat tggcggcaca
     1381 aaataaagaa caattttgaa ttccttacat tcctggctat tgcacaactg aatttaaggc
     1441 tctattatta cctcaacaaa ccaccccaat ataagtttga gattactaca atgagcgaag
     1501 cacttaaaat tctgaacaac atccgtactc ttcgtgcgca ggcaagagaa tgtacacttg
     1561 aaacgctgga agaaatgctg gaaaaattag aagttgttgt taacgaacgt cgcgaagaag
     1621 aaagcgcggc tgctgctgaa gttgaagagc gcactcgtaa gctgcagcaa tatcgcgaaa
     1681 tgctgatcgc tgacggtatt gacccgaacg aactgctgaa tagccttgct gccgttaaat
     1741 ctggcaccaa agctaaacgt gctcagcgtc cggcaaaata tagctacgtt gacgaaaacg
     1801 gcgaaactaa aacctggact ggccaaggcc gtactccagc tgtaatcaaa aaagcaatgg
     1861 atgagcaagg taaatccctc gacgatttcc tgatcaagca ataatctttt gtagattgca
     1921 cttgcttaaa atcccgccag cggcgggatt ttttattgtc cggtttaaga caatttaata
     1981 agaataagct ataaaaaaac ggcgtcgatt gctcaacgcc gtttcgtgga taacaccgat
     2041 acggatgtta cttcttaatg cccatctctt cttcaagcca ggctttaaat tccgtgccaa
     2101 gggtgttatg acgaataccg tattcaacga aggcctgcat gtaacctaat ttattaccgc
     2161 agtcatggct cttccctttc atatgatagg cttccaccgt ttctttttcg atcagcatat
     2221 caattgcgtc ggtgagctga atttcatcac cagctcccgg aggggttttt gccagcaacg
     2281 gccaaatatc cgcgctaagt acgtaacgac ccacaatagc gagattagac ggcgcaacat
     2341 ccgctttcgg tttttctacc acaccaacca tcggtacgct ttcacccggc gctaattcaa
     2401 cgcctttgca atccacaacg ccatatgcgg tcacatcagc aaccggttca accatgatct
     2461 ggctatgacc cgtttcatca aagcggcgga tcatctctgc caggttatcc tgtgacaaat
     2521 cggattcata ttcatccaga ataacatcag gcaaaataac agctaccggt tcatcaccca
     2581 ctaccgggtg agcacacaat accgcgtgtc ccaggccttt cgccagaccc tgacgaactt
     2641 gcataatagt cacgtgcggt ggacaaatag actgcacttc atcaagcagt tgacgtttta
     2701 cacgtttttc cagcattgcc tccagttcaa aactggtatc aaagtggttt tcaatagagt
     2761 ttttagatga gtgtgtaacc agcacaattt cagtaatgcc agccgcaata cattcattca
     2821 cgacgtattg aattaatggc ttatcgacaa gtggcagcat ctctttcggg atggctttcg
     2881 tcgccggcaa catcctggtt cctaatcccg caacggggat aacggctttt ttgactttcg
     2941 tattaatggc agccatttaa attctcctgg actgttcgtg ttttgaacgt gttcatattt
     3001 ctgtatcgca tcccagtata tcagcacccc gaaggggcta agttctgaaa aggaggcgag
     3061 atagcataat taagacaatt acttatacga atggcgacga tagtaccacc agccgacatt
     3121 agcaggtaat gcaaatttag cccgcgttat cgtttgctca ttctgcagac aacatcaagc
     3181 gcagtcgacc accggttccc catatttggc attgccaggc atcgcatcgc tggctcaatt
     3241 gattcaaata agcgttacct aaagtgccta acggaacacc attactgatt tgcacctggt
     3301 gttcgccggt atttaacgtc gcattcagac ccgcagaaac cagaatgaga tttttcagtt
     3361 cgcgatgata atagccaact aatagcggaa actgccccgg cagattggcc tgacgaagta
     3421 aatggtttac ctgcttcaat aacgcgccca actctggcaa ccgttgattt tggtgtgcaa
     3481 gctgttcctg taataatccg ttaaacaatg cgcgtaataa caaggcagca agtacgccat
     3541 tatgtccagc tcgggtgaca tcaaggcaat aaaatgccag atcgttttcc gaaagtgcgg
     3601 caatatcaag caccaggccg ggtttgtccg cggcaaccaa ttgacggtaa ttaacccggc
     3661 aatgggaaat cacctgctga accggcggtt gtagttcctg taataatttc gccgctgcgg
     3721 cagggttatc aaccattgca tcccagtcgt gaaaaagcct ttcctcttcc tcaacgcgcg
     3781 aattaaacat gctgggatag agacaggcaa aaaccatctc gcgcaagcga ttcagatctt
     3841 taactggttt cagcaaaaca tcttcaacgc ccagacgtaa cgctttggca atatctgcca
     3901 tattttcagt ggcagatatc accagaactg gggtctggtc gcctctgtta cgtatatgct
     3961 ccagcagttt aagcccgttc attcgtggca tcgcgatatc acatatcatc aggtctggag
     4021 tgaaacctcc cagcaactca agggcatcca ccccatcagc cgccagtacc gttgtcgctc
     4081 ccaatgagga aaaccatgaa tccagaagcg agcgaaatac ctgctcatct tcaacaatga
     4141 gaatctgttt tccgaccaat ggctgcgtca tgttctctcc cctgaccggc tttactcaat
     4201 agtggcatgc tattgccacc tgcgcctgtc agaattaact taagtgtaga taaaaattct
     4261 ggtcaaatgt tggtgcgtac caacggcaaa agttcgtcca ttttcctttc cactgccagc
     4321 tgtccggccg caatggcagc gtgcgcgcga tggaaatcaa gcgtagatat ttgcgggcaa
     4381 acaggttgaa tcagaatatc gggcggatca cctgccatgc ggttcctttt aaggcggttc
     4441 tccaacacct ggatagacgt ggtcataatc tctgttgccg ttggcgctgt cacagcccga
     4501 cgtgtcgtta tgctgcctaa cctttctttc agacgcgcat gccacggtag agaatcacca
     4561 ttctcgctat tttcttcact gacattaaag gagagcagat cttgttgcat caaatgagca
     4621 tcgtgctgca ggtcgaccgc tatcacaata tcagccccca atgcacgcgt aagagaaata
     4681 ggaattgggt taactactgc cccatcaacc agccagtagc cgttatgtgc aacgggtgcc
     4741 atgagtcctg gaatactgca tgatgcgcga atagcaagat ggagatcgcc ttcagtaaac
     4801 cataattcac gccccgtact taaattggtg gcaacagccg caaagcgacg ggaacaattt
     4861 tcgatctctg tttccggtat tatttcgcga tattgattga agacacgctc gccgcgcagt
     4921 aacccaccgc gctgccagga gaggtccatc aggcgtaaaa cgtcccaata actgaaagag
     4981 gttacccaat cttccagcgc agataatcga tcgcatgcat aggccgcgcc caccagcgaa
     5041 ccaattgaac atcctgcaac gatatcaatt tcaataccca ctttttttag cgcattaata
     5101 acgccaatat gcgaccaacc tctcgccgcg ccagatccca gcgccagccc tatttttatc
     5161 tttctcatta ttcccgcaag attcccctgg aatgcaatcg tagccacatc gctactgctc
     5221 ggttaacata gtgctactca ggcggttaat acgccgttat tgtttccagg gagattattg
     5281 gtgtctcagc tttgtccctg tggtagtgct gtcgagtata gcctatgttg ccacccttat
     5341 gtgtctggtg aaaaggttgc acctgatcca gaacatctca tgcgttcgcg ttactgcgct
     5401 tttgtgatgc aagacgctga ttatttaata aagacctggc atccctcttg tggagcagcg
     5461 gcgttacgtg ccgaattgat gaccggattt gcgcatactg aatggttggg attgagcgta
     5521 tttgaacatt gttggcagga cgccgacaat atcggatttg tcagttttgt tgctcgattt
     5581 accgaaggtg gcaaaacagg tgcaataatt gaacgttcgc gatttttgaa agaaaacggt
     5641 caatggtact atattgacgg tacacgtccg cagtttggtc gcaacgatcc ctgcccttgt
     5701 ggttcaggta aaaaatttaa aaagtgctgc ggccaataat ggttgacggt acggtttagc
     5761 aaacactctc aacaaggttt ttccagcaat gcattcactc caacgtaaag ttctgcgtac
     5821 tatttgtccg gaccaaaaag gtctgatcgc acgtattacc aatatttgct acaagcacga
     5881 gttaaatatc gtacagaaca atgaatttgt tgatcaccgt accgggcgct tttttatgcg
     5941 cacggaactg gaagggattt ttaatgattc caccctgctg gcggatctcg atagcgcatt
     6001 gccagaaggc tccgtgcgtg agctgaatcc tgccggtcgt cgccggatag tgattctggt
     6061 cactaaagaa gcgcattgcc ttggcgattt gttgatgaaa gccaattacg gcggtctgga
     6121 tgtcgaaatc gcggcagtta ttggtaacca cgatacttta cgttctctgg ttgagcgttt
     6181 tgatattccg tttgagctgg taagccatga agggttaagc cgcaacgagc acgatcaaaa
     6241 gatggcggac gccattgatg cttatcaacc tgactatgtg gtgctggcga agtatatgcg
     6301 ggtattaacg ccggaatttg tggcacgctt cccgaataaa atcatcaata ttcaccattc
     6361 attcctgcct gcgtttatcg gcgcacgtcc ttatcaccag gcctatgaac gtggtgtgaa
     6421 gattattggc gcaaccgctc actatgtgaa tgacaatctg gacgaaggcc caatcatcat
     6481 gcaggacgtt attcatgtcg atcataccta cacagctgaa gatatgatgc gcgcaggtcg
     6541 tgacgtcgag aaaaacgtct taagtcgtgc gctatacaaa gtactggcgc aacgcgtctt
     6601 tgtttacggt aatcggacga ttattcttta atcgccagca aaaataactg gttaccttta
     6661 atccgttacg gatgaaaatt acgcaaccag ttcatttttc tcaacgtaac actttacagc
     6721 ggcgcgtcat ttgatatgat gcgccccgct tcccgataag ggagctggct agtaaaaagc
     6781 attaccccgt ggtggggttc ccgagcggcc aaagggagca gactgtaaat ctgccgtcat
     6841 cgacttcgaa ggttcgaatc cttcccccac caccatcact ttcaaaagcc ccggaattct
     6901 caaacgaatc tgcaatcaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttgaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgtttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca gcaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacatac gacgtttcag caaaagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcaacatacc gaagaagtga ccgacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcggcc acggtcgaac agttcgctgt actggacctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcgatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 agccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaaca gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagcgcacg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaattccgtt gctgcccagc tcgcccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccatcgc cattttataa accggcgact
     9181 gctgtgcagc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacgtcca gctgacgctg gtagagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacggac
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt atcaaactca ccgcccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgataat
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcaaagattt
     9901 tacccagcag catggctcgg ttacccatgc gcggttgcag tttgccattg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tggcccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagtgc accgttgctg ttgaagactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgcgga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagcgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttt gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccgttaccg attttctcca tcagcgggcc gatagaggta aagcgttcgt aagttgccgg
    11221 ataatcgcgc tctacgacca taatgtgcgg tgcagttttg cctgggatca ggtcgcattc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcgtgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgctttta gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaggtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtggtcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaagaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagttacag cataacatgc tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcaaatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taacactaat
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcggcatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaagtaat cactacacag
    14341 gcgatataga aaatcaaaaa tactttcatt gcacctactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatcg cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgcc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcata gcaaagcccg cggagaagcc gatgaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa accgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccaacgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttgcggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtta tgacagctcc agtagccctt
    15661 tcggaggcag atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgga gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt agaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccggctat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatatatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aaggggttta
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16201 gttggcggca gtgccattaa gcgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttga ctcgagcagc agaacgagac ggacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgcg ctgatgcgtg cacaaaaccg
    16381 agaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgtattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagc catcgcgatt ttacccaacg
    16621 cgcaaacatc agcgggcgca acgaaatggc gatgcttgga actgcgttga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc ggtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaactgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actggtggat accctggttg aacaacttac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggaag cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgtct ggtgccttca catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaggtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tggtattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggctatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cttggttatc ttccaccacc acagacaatc gccagtgatt gcttgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgca tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tgtcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg tacaccaaag cggtaattaa gattcagccc gaggttattt
    18961 tgattttcgc cactttcccc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc aaagttaaac
    19081 aaatcaacac gatcaccaaa atactgttct aggctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgagc tgtcaggtcg tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgataa tcgcaaatat
    19261 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtcctg caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccattatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg attttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgctacaa gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aatgtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgcaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctctgattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgttta
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccttaaca
    20461 caccatccgg ataattctcc gccagtagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcat
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcatgatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcatcagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccagaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgcgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtatggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacatttcc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagtagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtttt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccaggcc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc ctgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aacgccagct cgctggagac attgctcgac agcatgaatg cccctgttgc
    22561 ctttactggc ttcctggtgg cactgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg ttcagcgcgc gatgaatctg ttctttggtt cagtgttagc
    22681 aacaatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 gcagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt actcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggagagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct
    23161 ggtggcattg gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaac
    23221 aaaacggggg ctaatcttga aaccagacac ttcaagaata gccgcaaaat gttgtcatta
    23281 caacaaggcg gctatatgac gctcgcgcag tttgccatga ctttctggca cgacctggca
    23341 gcaccgatcc tggcggggat tattaccgca gcgattgtca gctggtggcg taaccggaag
    23401 taaggtgaaa acgggcgtca ggctgccgtg atggcaatat gcgcccgaaa ccggccgcag
    23461 gggtaacagc ctgcggcctt ttttgtatct ggcacccatg aaaaaagtct taacgcagaa
    23521 cgctaagact ttatttttca agcaaaaaag attacttgct ggtatccagt tcttcgaaac
    23581 ctttcaccag atcatcaatc gctttcatct gcttgaggaa cggttccagt ttagccagcg
    23641 gcagcgcgga tggaccatca catttcgcat gttccggatc cggatgcgct tcaataaaca
    23701 gccccgccag acctaccgcc ataccggctc gtgccagctc agccacctga gcacgacgac
    23761 caccggaagc tgcgccaaac ggatcgcggc attgcagtgc gtgggtcacg tcgaaaatca
    23821 ccggcgagtt accagacact ttcttcataa tgctgaagcc cagcatatcg acaaccaggt
    23881 tgtcatagcc gaagttagca ccacgatcgc aaagaatcac tttttcgttg ccgccttctt
    23941 tgaatttatc aacgatatta cccatctgtc ccgggctgac aaactgtggt ttcttgacgt
    24001 taattaccgc accggttttc gccatggctt caaccaggtc agtctggcga gcaagaaacg
    24061 ccggcaactg aatcacatcc acgacatcag caacgggctg tgcctgactt ggttcgtgaa
    24121 cgtcggtgat aattttcacg ccaaaagtct gcttcaactc ctggaagatt ttcatccctt
    24181 cttccaggcc cggtccacga taagagtgga tggaggagcg gttggctttg tcaaaagagg
    24241 ctttgaacac gtaagggata cccagtttct gggtcacagt tacgtagtgc tcgcaaatgc
    24301 gcatcgccag atcgcgagat tccagcacgt tcataccgcc aaacagtacg aacggcaggt
    24361 catttgctac gttgatgtcg ccaatgctaa ccactttttg tttcatagga tcgccttaat
    24421 cttgagtaaa atgtcgatta attaatgcag cacaatatgt ttatgcgcga tgttatttat
    24481 ttgcgcacgg atcatttcgc tgatcgggtc ttccggacac tgttcaacga aataacttaa
    24541 atcgttcaac gcaacgtgtt cgcaatccag ttgcgcataa atcaacccgc gatcgcgaat
    24601 ttcataggga tcttcagggt tgaattgtaa taaagcttcg ctggtgcgta acgccagctc
    24661 catctgattt tcttccatca acgaggcttt gagtgtatcc agcaatttgc ggattacctc
    24721 aatgttatca gcttcatcaa ggtcttcata aaacagttcc gccgacgggc tgatatttcc
    24781 ctttaaccat acgtccagca tatgttcgct taacgattca ccgttaaaag gattaatcag
    24841 ccaaatttcg ccatccggac attcaatgcg caatatcagc tgcgtaggga aaatcaccgg
    24901 cagcagcggc aaatcgagac gattcgcgac ccataataaa accgcaccta atgataccgc
    24961 actgccctgt cgattcttta acacctggtc cagccataat gcatcggaaa gacgataaac
    25021 gccgcgtgag gctttaaatc cccagtcgcc gtaaaacagc gcgatcagtt tttccagctg
    25081 ctcttctaaa ggcagaagct ggctgatttc ttccttcgcc agactaacga gacgctccag
    25141 ttcgtcgtaa acatcttgcg agggaaaatc gcggcggatt gcttcgcaag ccaggatcat
    25201 gccttcgcac aatggcgctt tattaaattc gaaatcagct aacgatctca tgacttaccc
    25261 cagtaacggt acttttgtgg tggcgagttt aatgatgatg tacagcacca ccaacgccag
    25321 cgggaaggca ataatgcgcg cctgctgact gtgcatacga cgataatcga gtgcaataaa
    25381 acccaaaacg atataaatga taactccaaa cagcttttca gtcagccact gtgcctgccc
    25441 ggaaaatggc aggatgtgcg ctttagccat caacgcaatg ccgcttaaca ataacaccgt
    25501 atcgacaacc ggcggcacaa tgcgcgtcca gcgagcaaat gcctgtggat gcttctgata
    25561 acgtagccag aaacgtaagg ttaatagccc aacagaaagc gcgatactaa taagatgaac
    25621 actaagcagt gtagaaaaac ttgtcattga taatagcggc cgagcgttac gcgctcgtta
    25681 tcaccatagt cacggcaggt ttcgacgtca tgataccccg cgaggataaa tgcttgtcgc
    25741 accgcttcgc cctgctgcca gccatgttcc agaagcagaa agccgccgga taccagcgcg
    25801 ttacgcgact gttcgatgat atgcacgatg tctgccattc cactgtctgc cgcaaccagc
    25861 gcagtgagcg gctcaaagcg gacatcgcct tgttgaagat gtgggtcctg ctcgtcaata
    25921 tacggcggat tgctgacaat catcgcaaac tgctgcccgg ctagcgcgct aaaccagtcg
    25981 ctttgcagaa tgtggatatt tttgatcgcc agatgctggg cattacgttg tgccagggag
    26041 acagcatcag gcatacgatc tacagcgata atttcgcagt ccgggcgctc gctagccagc
    26101 gcaagcgcaa tcgccccggt acccgtcccg agatcgagaa tacggcaagg ttgttcaggc
    26161 aaccgcgcca gtgcctgctc caccagacac tccgtatccg ggcgcggaat taaggtcgct
    26221 ggagaaacaa ataacggcaa cgaccagaat tctcgcaccc cggttaaatg agcaatgggt
    26281 tcaccatcgc gacgacgtgt cagtagcgca tcaagttgct gacattgttc gtcagtcagc
    26341 tgcgtttcac caaaggcgag aataaaagta cgccctctgc cggtaacatg ctccagcagg
    26401 atttcagcat cacgccgcgg gctttcgctc gcctgaagtt ggcttattgc ttcacgtaac
    26461 cagtgttgat attccattat tcctgctcgg acaacgccgc cagttggtcg gcctgatgtt
    26521 cctggataat cggttcaatc agcatatcca gcttaccttc catcacttca tccaggcggt
    26581 agagcgtcag gttgatgcgg tgatcggtaa cgcgcccctg cgggaaattg taagtacggt
    26641 tacggtcgct gcgatcgcca ctccccagca ggttacgacg ggtagacgct tcggcctgtt
    26701 ggcgttttgc catttcagca gcgtggatgc gagcaccgag cactgaaagc gccttcgctt
    26761 tgtttttatg ttgtgaacgt tcgtcctgac attcaacaac aatcccggtc ggcaagtgag
    26821 taatacgaat tgccgaatcg gtggtgttaa cgtgctgacc acccgcccct gacgagcgga
    26881 aagtatcaat gcgtaaatct gctgggttga tgtccggcag ttctgcgtca ggcagttctg
    26941 gcattaccgc aacggtacaa gcagaagtat gaatacgacc ctgcgattcc gtagcaggaa
    27001 cacgttgcac gcgatgaccg ccggattcaa atttcagacg accatacaca ccatcaccgc
    27061 taattttggc gatgatctct ttataaccac catgttcacc ctcgctggcg ctcatgattt
    27121 ctacccgcca gcggcgggct tcggcgtaac ggctgtacat acggaacaga tcgcctgcaa
    27181 acagcgctgc ttcgtcgccg ccggttccgg cacggacttc gaggaaggcg ttacgttcgt
    27241 catcaggatc ttttggtagt aatagaacct gtaattgctg ttccagttgc tcgctttttt
    27301 ctttagcttc gcgcagttca tcctgcgcca tctcacgcat ttcaggatcg tcgagcatca
    27361 tctgtgcggt ttcgatatct tcctgaacct gttgccagtc ggtaaaacag cgcgaaacat
    27421 cacttaactg cgcatattcg cgtgataatg cgcgaaaacg ttcctggtcg gcgatagttt
    27481 gcgcgtcacc cagcaacgcc tgaacttctt catggcgttc atgcagggct tccagtttgg
    27541 caacgataga aggcttcata ggcgtaaatg caccctgtaa aaaaagaaaa tgatgtactg
    27601 ctactccagc ccgaggctgt cgcgcagaat attcaggcgt tcgttatccc cgtcacgggc
    27661 ggcctgttga agtgatttcg ttggcgcatg gatcaagcgg ttagtcagtt tccatgccag
    27721 atcctgcata atggcttgcg cgtcgccgcc ctgctcaagg gccgctaacg ctttggcggt
    27781 taactcatcg cgaacgtgct ctgcctggct gcgatactcg cgaatggttt cgctggcgct
    27841 ttgtgctcgc agccacgcca taaattcgct ggtttcctga gcgacaatag tttccgcctc
    27901 aaccgctgcg gctttacgct gcgccaggtt gtgcgaaatg atgctttgca gatcgtccac
    27961 actataaaga taagcattcg ccagtttgcc aacttccggc tcaacatcgc gcggaacggc
    28021 aatatccacc aacagcattg gttggttacg acgacttttt aatgcgcgct ccaccatgcc
    28081 tttcccgata atcggtaacg ggctggcggt ggaactgatg atgatatcgg cttcgcgcag
    28141 acgttcgtcg atctcactca gggcaatcac ttccgcgcca acttcatctg ccagaatttg
    28201 ggcacgttcg cgagtgcggt tggcgataat catcttctgt actttatgtt cgcgcagatg
    28261 acgcgctacc agctcgatgg tttcgcccgc gcctaccagc aacactgtga ccgtagagag
    28321 cgattcaaag atctgccgcg ccagcgtaca agccgcaaaa gcgacagaca cagcactggc
    28381 accgatatct gtttcagtgc gaacgcgttt cgctacagag aaagatttct ggaacatgcg
    28441 ttccagttcg ctggccttca tatgaccttt ttgcgaatcg gcaaacgctt ttttaacctg
    28501 accgaggatc tgcggctccc caagaaccag tgaatccagg ccgctggcaa cacgcattaa
    28561 atggctaacc gcgtcgttat cctgatgcca gtagaggttt ttacgcagat cttcttcatt
    28621 aagattgtga taatcgcaaa gccagcggat taacgcctct tgcaggttat cctgctcttc
    28681 aacgctaaga taaagttccg tgcggttgca cgtcgacagc accacgccgc cctgcaccat
    28741 cggctgcgca agcaggctgt caagcgcctg atcgagctta tccggcgaaa acgatacacg
    28801 ttctcgcagc gatacaggtg ccgttttatg gttgataccg agtgctaaaa gggtcatgtc
    28861 tgcgggaaat aataccaacg ttgatagggt tagtctgctt gcatcataca ggatgcgtaa
    28921 gatcaataaa agagagcgcc cccttttgga gtaattgccg aaagccgtta gattctggca
    28981 attaagacaa cttgaacata gacgatagcg gacggtaacg ctagcattaa gggttataac
    29041 tgcaacgtat ctcaaggact tgtcatcact atgcccctgc ccgattttcg tcttatccgc
    29101 ctgctaccgc tggctgctct tgtgctcact gcctgttccg ttaccacgcc caaaggtcct
    29161 ggcaaaagcc cggattcgcc acaatggcgt cagcatcagc aagacgtgcg caatcttaat
    29221 cagtatcaga ctcgcggcgc gttcgcttat atttctgacc aacaaaaagt gtacgcccgc
    29281 tttttctggc agcaaaccgg ccaggatcgc taccgtctgc tgctcactaa cccattgggc
    29341 agcacggaac tggagctgaa tgctcaaccg ggtaacgtgc agttagtcga caataaaggt
    29401 cagcgttata ccgccgatga cgccgaagag atgattggca aattgaccgg aatgccaatt
    29461 ccgctcaaca gcttgcgcca gtggatttta ggtttaccgg gtgatgcaac cgactacaaa
    29521 ctggacgacc agtaccgcct gagcgaaatt acctacagcc agaatggcaa aaactggaag
    29581 gttgtttatg gtggttatga caccaaaacg caacctgcga tgccagccaa tatggaactc
    29641 accgacggtg gtcaacgcat caagttaaaa atggataact ggatagtgaa ataatgcgga
    29701 cacagtggcc ctctccggca aaacttaatc tgtttttata cattaccggt cagcgtgcgg
    29761 atggttacca cacgctgcaa acgctgtttc agtttcttga ttacggcgac accatcagca
    29821 ttgagcttcg tgacgatggg gatattcgtc tgttaacgcc cgttgaaggc gtggaacatg
    29881 aagataacct gatcgttcgc gcagcgcggt tgttgatgaa aactgcggca gacagcgggc
    29941 gtcttccgac gggaagcggt gcgaatatca gcattgacaa gcgtttgccg atgggcggcg
    30001 gtctcggcgg tggttcatcc aatgccgcga cggtcctggt ggcattaaat catctctggc
    30061 aatgcgggct aagcatggat gagctggcgg aaatggggct gacgctgggc gcagatgttc
    30121 ctgtctttgt tcgggggcat gccgcgtttg ccgaaggcgt tggtgaaata ctaacgccgg
    30181 tggatccgcc agagaagtgg tatctggtgg cgcaccctgg tgtaagtatt ccgactccgg
    30241 tgatttttaa agatcctgaa ctcccgcgca atacgccaaa aaggtcaata gaaacgttgc
    30301 taaaatgtga attcagcaat gattgcgagg ttatcgcaag aaaacgtttt cgcgaggttg
    30361 atgcggtgct ttcctggctg ttaaaatacg ccccgtcgcg cctgactggg acaggggcct
    30421 gtgtctttgc tgaatttgat acagagtctg aagcccgcca ggtgctagag caagccccgg
    30481 aatggctcaa tggctttgtg gcgaaaggcg ttaatctttc cccattgcac agagccatgc
    30541 tttaagccgg gcaagctgag tttcggtgac aacgtcacct tgttccagac gttgcatcgc
    30601 gctctttaat acaccgcctg gaaaggatca tgcctggccc gcacagtttt cggcagattc
    30661 tttccaccaa tggacgcatg cctgaggttc ttctcgtgcc tgatatgaag ctttttgctg
    30721 gtaacgccac cccggtacta gcacaacgta ttgccaaccg cctgtacact tcactcggcg
    30781 acgccgctgt aggtcgcttt agcgacggcg aagtcagcgt acaaattaac gaaaatgtac
    30841 gcggtggtga tattttcatc atccagtcca cttgtgcccc tactaacgac aacctgatgg
    30901 aattagtcgt tatggttgat gccctgcgtc gtgcttccgc aggtcgtatc accgctgtta
    30961 tcccctactt tggctatgcg cgccaggacc gtcgcgtccg ttccgctcgt gtaccaatca
    31021 ctgcgaaagt ggttgcagac ttcctctcca gcgtcggtgt tgaccgtgtg ctgacagtgg
    31081 atctgcacgc tgaacagatt cagggtttct tcgacgttcc ggttgataac gtatttggta
    31141 gcccgatcct gctggaagac atgctgcagc tgaatctgga taacccaatt gtggtttctc
    31201 cggacatcgg cggcgttgtg cgtgcccgcg ctatcgctaa gctgctgaac gataccgata
    31261 tggcaatcat cgacaaacgt cgtccgcgtg cgaacgtttc ccaggtgatg catatcatcg
    31321 gtgacgttgc aggtcgtgac tgcgtactgg tcgatgatat gatcgacact ggcggtacgc
    31381 tgtgtaaagc tgctgaagcg ctgaaagaac gtggtgctaa acgtgtattt gcgtacgcga
    31441 ctcacccgat cttctctggc aacgcggcga acaacctgcg taactctgta attgatgaag
    31501 tcgttgtctg cgataccatt ccgctgagcg atgaaatcaa atcactgccg aacgtgcgta
    31561 ctctgaccct gtcaggtatg ctggccgaag cgattcgtcg tatcagcaac gaagaatcga
    31621 tctctgccat gttcgaacac taatcgaacc cggctcaaag acccgctgcg gcgggttttt
    31681 ttgtctgtaa tatccatttg tatgacctat gcctccttca cctgccattt agttgacaga
    31741 tgatgcgctc acggatgaaa cattattgtg aacaaaatat tttcctcaca tgtgatgcct
    31801 ttccgcgctc tgatcgacgc ttgctggaaa gaaaaatata ctgccgcacg gtttacccgt
    31861 gacctgattg ccgggataac cgtcgggatt attgctatcc cgctggcgat ggcgttggct
    31921 attggtagtg gtgtggcacc ccagtacggt ttatataccg cagctgttgc ggggattgtc
    31981 attgctctaa cgggtgggtc acgctttagc gtttccggtc cgactgcggc atttgtggta
    32041 attctctatc ccgtttcgca acagtttgga ctggcaggac tgctggttgc gaccttgttg
    32101 tcgggaatct ttttgattct gatggggctg gcacgctttg gtcgtctgat tgagtatatt
    32161 ccggcttccg tcaccttagg tttcacctcg ggtatcggga tcaccatcgg taccatgcag
    32221 attaaagatt ttctcggtct gcaaatggcc catgtcccgg aacattatct acaaaaagtc
    32281 ggcgcattat ttatggcgct gccgaccatt aatgtgggtg atgctgccat tggcattgtg
    32341 acgcttggca ttctggtttt ctggccgcgt ctgggcattc gtttacccgg tcaccttccg
    32401 gccttgctgg ctggttgcgc ggtgatgggg attgttaacc tgctcggcgg acatgttgct
    32461 accatcggtt cgcaattcca ctacgtcctg gccgatggtt ctcagggtaa cggtattccg
    32521 caactgctgc cgcaactggt gctgccgtgg gatctgccta attcagaatt cacgctaacc
    32581 tgggattcta ttcgcaccct gctgcctgcg gcattctcaa tggcaatgct cggggcgatc
    32641 gaatctctgc tctgcgctgt ggtactggat ggtatgaccg ggactaagca caaggcgaat
    32701 agtgaactgg tcggtcaggg gctggggaat atcatcgctc cattctttgg tggtattacc
    32761 gctactgctg ccatcgcgcg ttctgccgct aacgtccgtg ccggggcaac ttcccctatc
    32821 tcggcggtga tccactctat tctggttatt ctcgccctgc tggtactggc accactgctc
    32881 tcctggctgc cgctttccgc catggcagcc ctgctgttga tggtggcgtg gaacatgagt
    32941 gaagcgcaca aagtggtcga cttgctgcgt catgcgccga aagatgacat catcgtcatg
    33001 ctgctgtgta tgtcactgac cgtgctgttt gatatggtta ttgccatcag cgtggggatc
    33061 gtgctggcat cgctgctgtt tatgcgtcgt atcgcacgta tgactcgcct ggcaccggta
    33121 gtcgtagatg ttccagacga tgtcctggtt ctgcgcgtta ttggcccgct gttttttgct
    33181 gctgctgaag gcttattcac ggacctggag tcacgtcttg aaggcaaacg gattgtgatt
    33241 ctgaagtggg atgccgttcc ggtacttgat gctggtggtc ttgatgcgtt ccagcgtttt
    33301 gtgaagcgtc tgcccgaagg atgtgaactg cgcgtgtgca acgtggaatt ccagccactg
    33361 cgaactatgg ctcgcgcagg cattcaaccg atcccgggac gcctcgcgtt cttcccgaat
    33421 cgtcgcgcgg cgatggcgga tttataagta cgagattgac cagtcagcgc aaactgactg
    33481 gtcagcaaac tgcattattt gttagctatg acggcgatta tcgctacggc ggcaacgttt
    33541 gtcatagtga cgaacccacc agtaacggtc gcacacctgt tcatgcccgc caacacgggc
    33601 acccgccagc cagagaatgg caccgacgaa aatacttagc actgcaccat gtgcgaaata
    33661 ctggggcata ttaaactgtg gtaactggtt gaggattgaa taccccacgc cgaccaccat
    33721 taccaccaga cccaacccca tgagcacgtt accgagtatc gaagcgtttt tgcgtttcat
    33781 atgtcacctc cggaactttc tgggttgtaa cagggaatac ccctcttcct tatgtgtaaa
    33841 gtatagacaa caccctgtgg gttatgtgcg ggcgtaatca caattacaac ccttatttca
    33901 acaaaacttt acaaataaac gcctgaacac cttgactttc taatagtcca ctcaggcaat
    33961 tattacgtta tctgcatttg atacgcagtt ttttgtcaag cggggccgca accagtaaac
    34021 tacgcgccag ttatgtacac actcaggaca aaaaaacgtg acgattaaat tgattgtcgg
    34081 cctggagaac cccggtgctg aatacgccgc aacgcgacat aatgctggtg cctggttcgt
    34141 tgacttactg gcagagcgtt tgcgcgctcc gctgcgcgaa gaggctaaat tctttggtta
    34201 tacttcgcga gtcacccttg gaggcgaaga tgtccgcctg ttagtcccga ctacatttat
    34261 gaatctcagc ggcaaagccg ttgcggcgat ggccagtttt ttccgcatta atccggacga
    34321 aattctggtg gcccacgacg aactggatct gcctcctggc gtcgccaaat ttaagttggg
    34381 cggtggccat ggtggtcaca atggactgaa agacatcatc agtaaattgg gtaataaccc
    34441 taactttcac cgtttacgca tcggaatcgg tcatccgggc gataaaaata aagttgtcgg
    34501 ttttgtgtta ggcaaaccgc ctgttagtga acagaagtta attgatgaag ccatcgacga
    34561 agcggcgcgt tgtactgaaa tgtggtttac agatggcttg accaaagcaa cgaaccgatt
    34621 gcacgccttt aaagcgcaat aagtcgttgt ctgcggcatt tttgccgagt gccgtgtata
    34681 ataggcaaag ttatttccat ttctgcaatc tgttagcaat aacaggttga ttattaagat
    34741 attaaggtga tttaaatcat gggattcaaa tgcggtatcg tcggtttgcc caacgtcggg
    34801 aaatctaccc tgttcaacgc gctgaccaaa gccggtattg aagcggccaa ctttccattc
    34861 tgcaccattg agccgaacac aggcgtcgta ccaatgcctg accctcgcct ggatcaactg
    34921 gctgaaatcg taaaaccgca acgtacgctt cccacgacca tggaatttgt cgatatcgcc
    34981 ggtctggtaa aaggcgcatc gaaaggcgaa ggtctgggta accagttcct gaccaacatc
    35041 cgtgaaaccg aagcgatcgg tcacgttgtt cgctgctttg aaaatgacaa catcattcac
    35101 gtttccggca aagttaaccc ggctgacgat attgaagtta tcaacaccga actggcgctg
    35161 gcagacctcg acacctgcga acgtgcgatt catcgcgtac agaagaaagc caaaggtggc
    35221 gataaagacg cgaaagctga gctggcggtc ctggaaaaat gcctgcccca gctggaaaac
    35281 gcaggtatgc tgcgcgcgct ggatttaagc gctgaagaga aagcggctat tcgttacctg
    35341 agcttcctga cgctgaaacc aacaatgtac atagccaacg tcaacgaaga cggttttgaa
    35401 aacaacccat atcttgacca ggtgcgtgaa atcgcggcga aagaaggttc tgttgtggtt
    35461 ccggtttgtg ctgctgttga agcagacatt gccgaactgg acgacgaaga acgtgacgag
    35521 tttatgcagg agcttgggct ggaagaaccg ggcctgaacc gtgtgatccg tgccggttat
    35581 aagctgctga acctgcaaac ttacttcacc gctggggtga aagaagtgcg tgcatggacc
    35641 attccggttg gagcaaccgc gccgcaggca gcgggcaaaa tccatactga ttttgaaaaa
    35701 ggctttatcc gtgcacaaac catctcgttt gaagatttca tcacttacaa aggtgaacaa
    35761 ggcgcgaaag aagcaggcaa aatgcgtgca gaaggtaaag attacatcgt gaaagatggc
    35821 gatgtgatga acttcctttt caacgtctaa ttaatttatt gtctcatgag gtttcgctaa
    35881 atctcatgaa gagcgcaaaa gtatataaaa tccacgcaat tgcgtggatt tttcatttca
    35941 ccgagtccta atcattcttg taa
//
LOCUS       DADMEQ010000028        35272 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.028, whole genome shotgun sequence.
ACCESSION   DADMEQ010000028 DADMEQ010000000
VERSION     DADMEQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.028"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            606..2891
                     /gene="nrdA"
                     /locus_tag="JNZ05_001439"
     CDS             606..2891
                     /gene="nrdA"
                     /locus_tag="JNZ05_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HAY8564722.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3125..4255
                     /gene="nrdB"
                     /locus_tag="JNZ05_001440"
     CDS             3125..4255
                     /gene="nrdB"
                     /locus_tag="JNZ05_001440"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HAY8564723.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4255..4509
                     /gene="yfaE"
                     /locus_tag="JNZ05_001441"
     CDS             4255..4509
                     /gene="yfaE"
                     /locus_tag="JNZ05_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HAY8564724.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4563..5213)
                     /gene="inaA"
                     /locus_tag="JNZ05_001442"
     CDS             complement(4563..5213)
                     /gene="inaA"
                     /locus_tag="JNZ05_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HAY8564725.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSRVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5377..5619
                     /locus_tag="JNZ05_001443"
                     /pseudo
     CDS             5377..5619
                     /locus_tag="JNZ05_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5661..6737)
                     /gene="glpQ"
                     /locus_tag="JNZ05_001444"
     CDS             complement(5661..6737)
                     /gene="glpQ"
                     /locus_tag="JNZ05_001444"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY8564726.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6742..8100)
                     /gene="glpT"
                     /locus_tag="JNZ05_001445"
     CDS             complement(6742..8100)
                     /gene="glpT"
                     /locus_tag="JNZ05_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HAY8564727.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8373..10001
                     /gene="glpA"
                     /locus_tag="JNZ05_001446"
     CDS             8373..10001
                     /gene="glpA"
                     /locus_tag="JNZ05_001446"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HAY8564728.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIAHHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9991..11250
                     /gene="glpB"
                     /locus_tag="JNZ05_001447"
     CDS             9991..11250
                     /gene="glpB"
                     /locus_tag="JNZ05_001447"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HAY8564729.1"
                     /translation="MRFDTVIMGSGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNKENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQITQRAGGQQ"
     gene            11247..12437
                     /gene="glpC"
                     /locus_tag="JNZ05_001448"
     CDS             11247..12437
                     /gene="glpC"
                     /locus_tag="JNZ05_001448"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HAY8564730.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12630..13529
                     /locus_tag="JNZ05_001449"
     CDS             12630..13529
                     /locus_tag="JNZ05_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY8564731.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDESDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGFEREIVLAATQLTDADIPNCY"
     gene            13548..13769
                     /locus_tag="JNZ05_001450"
     CDS             13548..13769
                     /locus_tag="JNZ05_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564732.1"
                     /translation="MTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQQGMHEQAIKIA
                     LRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13810..14280)
                     /locus_tag="JNZ05_001451"
     CDS             complement(13810..14280)
                     /locus_tag="JNZ05_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY8564733.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14335..15537)
                     /locus_tag="JNZ05_001452"
     CDS             complement(14335..15537)
                     /locus_tag="JNZ05_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HAY8564734.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVSFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15624..16166)
                     /locus_tag="JNZ05_001453"
     CDS             complement(15624..16166)
                     /locus_tag="JNZ05_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564735.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16445..16870
                     /gene="nudI"
                     /locus_tag="JNZ05_001454"
     CDS             16445..16870
                     /gene="nudI"
                     /locus_tag="JNZ05_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HAY8564736.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16909..17511)
                     /locus_tag="JNZ05_001455"
     CDS             complement(16909..17511)
                     /locus_tag="JNZ05_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAY8564737.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17819..18958
                     /gene="arnB"
                     /locus_tag="JNZ05_001456"
     CDS             17819..18958
                     /gene="arnB"
                     /locus_tag="JNZ05_001456"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HAY8564738.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDTIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18962..19930
                     /gene="arnC"
                     /locus_tag="JNZ05_001457"
     CDS             18962..19930
                     /gene="arnC"
                     /locus_tag="JNZ05_001457"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HAY8564739.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19930..21912
                     /gene="arnA"
                     /locus_tag="JNZ05_001458"
     CDS             19930..21912
                     /gene="arnA"
                     /locus_tag="JNZ05_001458"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HAY8564740.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYDS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            21909..22799
                     /locus_tag="JNZ05_001459"
     CDS             21909..22799
                     /locus_tag="JNZ05_001459"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HAY8564741.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22799..24444
                     /gene="arnT"
                     /locus_tag="JNZ05_001460"
                     /pseudo
     CDS             22799..24444
                     /gene="arnT"
                     /locus_tag="JNZ05_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
     gene            24441..24776
                     /gene="arnE"
                     /locus_tag="JNZ05_001461"
     CDS             24441..24776
                     /gene="arnE"
                     /locus_tag="JNZ05_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HAY8564742.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24776..25162
                     /gene="arnF"
                     /locus_tag="JNZ05_001462"
     CDS             24776..25162
                     /gene="arnF"
                     /locus_tag="JNZ05_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HAY8564743.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25156..25428)
                     /gene="pmrD"
                     /locus_tag="JNZ05_001463"
     CDS             complement(25156..25428)
                     /gene="pmrD"
                     /locus_tag="JNZ05_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HAY8564744.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25533..26888)
                     /gene="menE"
                     /locus_tag="JNZ05_001464"
     CDS             complement(25533..26888)
                     /gene="menE"
                     /locus_tag="JNZ05_001464"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HAY8564745.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26885..27847)
                     /gene="menC"
                     /locus_tag="JNZ05_001465"
     CDS             complement(26885..27847)
                     /gene="menC"
                     /locus_tag="JNZ05_001465"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HAY8564746.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(27847..28704)
                     /gene="menB"
                     /locus_tag="JNZ05_001466"
     CDS             complement(27847..28704)
                     /gene="menB"
                     /locus_tag="JNZ05_001466"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HAY8564747.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28719..29477)
                     /gene="menH"
                     /locus_tag="JNZ05_001467"
     CDS             complement(28719..29477)
                     /gene="menH"
                     /locus_tag="JNZ05_001467"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HAY8564748.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGVAG
                     LCGVIIEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29474..31144)
                     /gene="menD"
                     /locus_tag="JNZ05_001468"
     CDS             complement(29474..31144)
                     /gene="menD"
                     /locus_tag="JNZ05_001468"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HAY8564749.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIGNIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31233..32528)
                     /gene="menF"
                     /locus_tag="JNZ05_001469"
     CDS             complement(31233..32528)
                     /gene="menF"
                     /locus_tag="JNZ05_001469"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HAY8564750.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAKGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGERLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32607..32912)
                     /gene="elaB"
                     /locus_tag="JNZ05_001470"
     CDS             complement(32607..32912)
                     /gene="elaB"
                     /locus_tag="JNZ05_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HAY8564751.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGEPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32967..33428)
                     /locus_tag="JNZ05_001471"
     CDS             complement(32967..33428)
                     /locus_tag="JNZ05_001471"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8564752.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDCIPHIGMAREVIQA"
     gene            33493..34410
                     /gene="rbn"
                     /locus_tag="JNZ05_001472"
     CDS             33493..34410
                     /gene="rbn"
                     /locus_tag="JNZ05_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HAY8564753.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34601..35272
                     /locus_tag="JNZ05_001473"
                     /pseudo
     CDS             34601..35272
                     /locus_tag="JNZ05_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         8671 a         9420 c         8715 g         8466 t
ORIGIN      
        1 tgcaatggct attttgttat tatttgatga ataatatcag tgcgtcataa ttcaagttaa
       61 taaccttaag ggataccagt tatatttaaa ctaaattaaa gtaatgaata attttcttat
      121 aatataaggt aaattaacaa aatggcttcg catttaacaa taaccgaata gaaaacaacc
      181 atttcgacat caacaatcgc ttacattcgc ttatatattg accacaactg atacatcaga
      241 ttatgtgatg actcgtgctt agatcaattt ttgcaatcat tagcaaaaag attaataaac
      301 catctatatc aatttatcca acctattctg ccgttcaaga aatcgccgaa cagttatttt
      361 taacaaattt ttctcttccc attgactttc ccggacacct tgtctgacct aaggtgcgcg
      421 aaagccactt tttccttcct gagttatcca caaagttatg cacttgcaag agggtcattt
      481 tcacactatc ttgcagtgaa tcccaaacat accccctata tatagtgttc taagcagctt
      541 cccttactac aggtagtctg catgaaacta ttgcggaaag aattccaaaa acaggtacga
      601 catacatgaa tcagaatctg ctggtgacaa agcgcgacgg tagcacagag cgcatcaatc
      661 tcgacaaaat ccatcgcgtt ctggattggg cggcagaagg actgcataac gtttcgattt
      721 cccaggtcga gctgcgctcc cacattcagt tttatgacgg tatcaagacc tctgacatcc
      781 acgaaactat tatcaaggct gccgcagacc tgatctcccg tgatgcgccg gattatcagt
      841 atctcgccgc gcgcctggcg atcttccacc tgcgtaaaaa agcctacggc cagtttgagc
      901 cgcctgcgct gtacgaccac gtggtgaaaa tggtcgagat gggcaaatac gataatcatc
      961 tgctggaaga ctacacggaa gaagagttca agcagatgga cacgtttatc gatcacgacc
     1021 gtgatatgac cttctcttat gctgccgtta agcagctgga aggcaaatat ctggtacaga
     1081 accgcgtgac cggcgaaatc tacgaaagcg cccagttcct ttatattctt gttgccgcgt
     1141 gcctgttctc gaactacccg cgtgaaacgc gcctgcaata cgtgaagcgt ttttacgacg
     1201 cggtttccac atttaaaatt tcgctgccga cgccaatcat gtccggcgtg cgtaccccga
     1261 ctcgtcagtt cagctcctgc gtactgatcg agtgcggtga cagcctggat tccatcaacg
     1321 ccacctccag cgcgattgtg aaatatgttt cccagcgtgc cgggatcggc atcaacgccg
     1381 gacgtattcg tgcgctgggc agcccgattc gcggtggcga agcgttccat accggctgta
     1441 tcccgttcta caaacatttc cagacagcgg tgaaatcctg ctctcagggc ggtgtgcgcg
     1501 gcggtgcggc aacgctgttc tacccgatgt ggcatctgga agtggaaagc ctgctggtgt
     1561 tgaaaaacaa ccgtggggtt gaaggcaacc gcgtgcgtca tatggactac ggggtacaaa
     1621 tcaacaaact gatgtatacc cgcctgctga aaggtgaaga tatcaccctg ttcagcccgt
     1681 ccgacgtacc ggggctgtac gacgcgttct tcgccgatca ggaagagttt gaacgcctgt
     1741 ataccaaata tgagaaagac gacagcatcc gcaagcagcg tgtgaaagcc gttgagctgt
     1801 tctcgctgat gatgcaggaa cgtgcgtcta ccggtcgtat ctatattcag aacgttgacc
     1861 actgtaatac ccatagtccg tttgatccga ccatcgcgcc agttcgccag tctaacctgt
     1921 gcctggaaat tgccctgccg accaaaccgc tgaacgacgt caacgacgag aacggtgaaa
     1981 tcgcgctgtg tacgctgtct gctttcaacc tgggcgcaat taatagcctg gatgaactgg
     2041 aagagctggc gattctggcg gttcgtgcac ttgacgcgct actggattat caggattacc
     2101 cgatcccggc cgccaaacgt ggagcgatgg ggcgtcgtac gctgggtatt ggtgtgatta
     2161 acttcgctta ctatctggcg aagcacggta aacgctactc cgacggcagc gccaacaacc
     2221 tgacgcataa aaccttcgaa gcgattcagt attacctgct gaaagcgtct aatgagctgg
     2281 ctaaagagca aggcgcgtgc ccgtggttta acgaaaccac ttacgcgaaa gggatcctgc
     2341 cgatcgatac ctataagaaa gatctggatg ccatcgctaa tgagccgctg cattacgact
     2401 gggaagcatt gcgtgaatca atcaaaacgc acggcctgcg taactccacg ctttctgccc
     2461 tgatgccgtc cgagacctct tcgcagatct ctaacgccac taacggtatt gaaccgccgc
     2521 gcggttacgt cagcatcaaa gcgtcgaaag acggtatttt gcgccaggtg gtgccggact
     2581 acgagcacct gcacgacgcc tatgagctgc tgtgggaaat gccgggtaac gatggctacc
     2641 tgcaactggt aggtatcatg cagaaattta tcgatcagtc gatctctgct aacaccaact
     2701 acgatccgtc acgtttcccg tcaggaaaag tgccgatgca gcagttgctg aaagacctgc
     2761 tcaccgccta caaattcggg gtcaaaacgc tgtattatca gaacacccgt gacggcgctg
     2821 aagacgcgca ggacgatctg gtaccgtcaa tccaggacga tggctgcgaa agcggcgcat
     2881 gtaagatctg atattgagat gccggatgcg gcgtaaacgc cttatccggc ctacggctcg
     2941 gtttgtaggc ctgataagac gcgccagcgt cgcatcaggc tccgggtgcc ggatgcagcg
     3001 tgaacgcctt atccggccta cggctcggat ttgtaggcct gataagacgc gccagcgtcg
     3061 catcaggcac aggatgcggc gtaaaatgcc ttatccggca ttaaactccc aacaggacac
     3121 actcatggca tataccacct tttcacagac gaaaaatgat cagctcaaag aaccgatgtt
     3181 ctttggtcag ccggtcaacg tggctcgcta cgatcagcaa aaatatgaca tcttcgaaaa
     3241 gctgatcgaa aagcagctct ctttcttctg gcgtccggaa gaagttgacg tctcccgcga
     3301 ccgtatagat taccaggcgc tgccggagca cgaaaaacac atctttatca gcaacctgaa
     3361 atatcagacg ctgctggatt ccattcaggg tcgtagcccg aacgtggcgc tattgccgct
     3421 tatttctatt ccggaactgg aaacctgggt cgaaacctgg gcgttctcag aaacgattca
     3481 ttcccgttcc tatactcata tcattcgtaa tatcgttaac gatccgtctg ttgtgtttga
     3541 cgatatcgtc accaacgagc agatccagaa acgtgcggaa gggatctcca gctattacga
     3601 tgaactgatc gaaatgacca gctactggca ccttctgggc gaaggcaccc acaccgttaa
     3661 cggtaaaacc gtaaccgtta gcctgcgcga gctgaagaaa aaactgtatc tctgcctgat
     3721 gagcgttaac gcgctggaag cgatccgctt ctacgtcagc tttgcttgtt cttttgcctt
     3781 tgcagaacgc gaactgatgg aaggtaacgc caagattatt cgcctgattg cccgcgatga
     3841 agccctgcac ctgaccggta ctcagcatat gctgaatctg ctgcgcagcg gcgcggatga
     3901 tccggagatg gcggaaattg ccgaagaatg taagcaggag tgctatgacc tgtttgtcca
     3961 ggcggcacag caagagaaag actgggcgga ttatctgttc cgtgacggtt cgatgattgg
     4021 tctgaataaa gatattctct gccagtacgt tgaatacatc accaatatcc gcatgcaggc
     4081 agtcggtttg gatctgccgt tccagacgcg ctccaacccg atcccgtgga tcaacacctg
     4141 gctggtgtct gataacgtgc aggttgctcc gcaggaagtg gaagtcagtt cttatctggt
     4201 cgggcagatt gacgcggaag tggacaccga cgatttgagt aacttccagc tctgatggcc
     4261 cgcgttaccc tgcgcatcac tggcacacaa ctgctgtgcc aggatgaaca cccttccctt
     4321 ctggcggcgc tggaatccca caatgtggcg gttgagtacc agtgccgcga aggttactgc
     4381 ggctcctgtc gcacccgact ggtcgcaggt caggtggact ggattgccga accgttagcc
     4441 tttattcagc cgggggaaat tttgccctgt tgttgccggg caaaaggcga tattgaaatc
     4501 gagttgtgat cgggtactag cgccagatac gacgcttacg cgtattatct ggcctacaac
     4561 gattacatgg cgtagtaata cgctttcacc tgctcccagt ccgctttagg gatcggctcc
     4621 agatattttt ccagctggcg gaagtcatga ttaatcgctt tatcgcgacg caagcggcga
     4681 cgacttttct ccagatcaag aaaaccagct tctgccttac cttctgtttt cacatagata
     4741 tggcgaacat agcaacagcc atgctgacga ttaacgctat gcattttctt aaacgccagt
     4801 gccactgctt tcaacatggc ttgccgtact tcgtcagaat aaggcgatac tgcatgccgg
     4861 gcataccagt cagcaatgct gatgaacccc gccatatctt cagtcaccag caacgcgcgc
     4921 cattcaccct caattttcac cgcttcacca aaaacgatct tcggcacgat gacaccggcc
     4981 cgttccagtt ctttaattac cgcaacctca cggacaatcg ttggtcggcc gaacggataa
     5041 cgtactgaat gaaacagatg atgcgtcatg cgctttacat acagcttttt gccgttgcgc
     5101 tcgacgcatt gcaccctgct cataccatta cggcgatagt taggctcttc aacccagtcg
     5161 ccctctgttg cccaccagtg attaaattcg tcgtactttg ctgaaactgc cataccctat
     5221 cgcctgtcat ttttattaac gacaatgact ataggtggtt acctgaggaa aatcttaatg
     5281 aaacgtgtcg tattccgcta caggtgtgac accgcttttg cccttcattt gcagcgcata
     5341 gattccacca gccactacca ctaatagtgc gcccagcaga taaaatgtga atttaatcag
     5401 tcggatacag cgatgaattt tattcgacag ggattaggca tcgccttaca accagagtta
     5461 acgctgaaaa gcattgcagg tgaattgtgt tccgttcctc acgaaccaac cttctatcga
     5521 cagatttcgt tgctgactaa agaaaagccg gtagaaggca gtccactgtt tttactacaa
     5581 atgtgcatgg aacaattagt ggcgattgga aaaatttgat atgagacagg atggcgcatg
     5641 aacgccatcc tgcagtaaca ttactcttta ttaagaaatt ttactgcctt atcagggaaa
     5701 tcagtaaaca gcccatttac acccgctttg ttatacagag catcatataa ctgattcaca
     5761 tcaggagtgt attcaggcag tttatctgac cgcacggtat aaggatgcac taccagttta
     5821 ttctgctgag catcttgcac catgccagtg agtttgatat tacccggctg cgatgtctcc
     5881 tcaatcaaca tatggtaatc cggaccaata ccatctgcat attccgccac ctgtttcatg
     5941 gcacccggct taaacatcca gtcgtagttg taattaaccc agcttccatc cggctgtttc
     6001 tgctgcgttt cattccagtc ggtataggca atcagctgta ccagattgag ctccatgccc
     6061 attttgggtt ccagctcatt cttaatacgc ttcagctcat cagcatcaaa acattgcaaa
     6121 taaactttat cgtctttacc ggtgtaacca tatttcttca gcacttccag cgtttttgcc
     6181 gcaatatcct tcccttcctg atgatggaac cacggcgctt tgatttctgg atagataccg
     6241 atatttttcc cggtagagtg atttaacccc tgaacaaatt caatctcttc ttcaaaggtg
     6301 tgcacccgga agtcggactt acccattggg aaacgccccg gataagtctg tactttttta
     6361 ccgttttcaa tatcgaaacc ttcggtaaat ttcagcgact taatttcatc cagcgtgaaa
     6421 tctatcgcgt agtaacgacc gtctttgcgc gcccgatccg ggaaacgatc ggcaacatca
     6481 gtaacacgat cgagataatg gtcatgcaga acaaccagat ggtcgtcttt ggtcatcacc
     6541 aaatcctgtt ccagataatc cgctccctgc gcatacgcca tcgcttttgc tggcagcgta
     6601 tgctccggca aatatccact ggcaccgcga tgggcgatga ctattttttc gttgctgtcc
     6661 gccgccattg cactgcttcc catgactata gtgctcatca tgatcgccat gctaaggttt
     6721 ttcagcttca atttcatgcc attagcctcc gttgcgtttt tgcagtaatt gttcatggcg
     6781 acgtttttcg ccaatcatca caacaatcaa caagataacc gccagaatgc tgccgccaat
     6841 cattaccata aagccgccat cccagccgaa gaagtccacg gtgtagccaa caatcgcgct
     6901 cgccgccacc gaaccgccca ggtaaccaaa cagcccggta aagcccgctg ccgtacctgc
     6961 cgcttttttc ggtgccagtt ccagcgcatg cagaccgatc agcatcacag gaccgtagat
     7021 caggaagccg ataacaatca tacaaatcat atcgacggtt gggttacctg ccgggttcat
     7081 ccagtaaacg atagtcgcga tggtcaccag tgtcataaag aagacgccgg ttgccccacg
     7141 gttaccacgg aagactttgt ccgacatcca gccgcacagc agggtgcccg gaatacccgc
     7201 atactcatag aagaagtagg cccaggagga tttatccagc gcgaaatgct taacctcttt
     7261 cagataagtc ggtgaccagt cgaggatgcc gtaacgcagc agataaacga acacgttggc
     7321 gatggcgatg taccacagca gtttgttcgg cagcacgtac tgcatgaaga tttgcttcgc
     7381 ggtcagctcc tgttccgctt tttcgttata gtcgtccgga taatcatttt tgtactcttc
     7441 gatcggcggc aagccacagg attgcggggt atcgcgcatc atcgcaaagg cgaataatgc
     7501 caccagaatg gcgcagaaag caggcatata gagcgccgca tgccagtcat tgaaccaggc
     7561 catccccagc aggaacagca gcggcggaat accaccaccg acgttgtgcg cacagttcca
     7621 cactgacaca atgccgccac gttctttctg cgaccaccag tgcaccatag tacgaccaca
     7681 cggcggccac cccatcccct ggaaccaacc gcagaggaac aacagtacaa acatcaccgc
     7741 aatgctcgac gtcgcccatg gcacaaagcc cataaacaac atcactgccg ccgccagaat
     7801 caaacctgcg ggcaggaaaa cgcgcggatt cgagcgatcc gataccgaac ccatgatgaa
     7861 tttcgaaaat ccataagcaa tcgagatccc cgaaagggca aaacctaaat caccgcgtga
     7921 gaatccctgc tcaaccagat aaggcatagc aagcgcaaag ttcttacgaa ccaaatagta
     7981 agccgcatag ccaaagaata tccccaggaa aatttgccag cgcaatcgac gataagtcgg
     8041 atcgatctcc gcggcaggta agcgcgcttt gtgtggcgct ggtttaaaaa tactcaacat
     8101 tgatagcctc cgtggcccgt ggtcttattt atgattaaca gcctgattca gtgagagaac
     8161 ctgccgtttc ttgagttgcc gcgatgttaa gaaaacattc ataaattaaa tgtgaattgc
     8221 cgcacacatt attaaataag atttacaaaa tgttcaaaat gacgcatgaa atcacgtttc
     8281 actttcgaat tatgagcgaa tatgcgcgaa atcaaacaat tcatgttttt actatggcta
     8341 aatggtaaaa aacgaacttc agagggataa caatgaaaac tcgcgactcg caatcaagtg
     8401 acgtgattat cattggcggc ggcgcaacgg gagccgggat tgcccgcgac tgtgccctgc
     8461 gcgggctgcg cgtgattttg gttgagcgcc acgacatcgc aaccggtgcc accgggcgta
     8521 accacggcct gctgcacagc ggtgcgcgct atgcagtaac cgatgcggaa tcggcccgcg
     8581 aatgcattag tgaaaaccag atcctgaaac gcattgcaca tcactgcgtt gaaccaacca
     8641 acggcctgtt tatcaccctg ccggaagatg acctctcatt ccaggccact tttattcgcg
     8701 cctgcgaaga agcagggatc agcgcagaag ctatagaccc gcagcaagcg cgcattatcg
     8761 aacctgccgt taacccggca ctgattggcg cggtgaaagt tccggatggc accgttgatc
     8821 catttcgtct gaccgcagca aacatgctgg atgccaaaga acacggtgcc gttatcctta
     8881 ccgctcatga agtcacgggg ctgattcgtg aaggcgcgac ggtgtgcggt gttcgtgtac
     8941 gtaaccatct caccggcgaa actcaggccc ttcatgcacc tgtcgtggtt aatgccgctg
     9001 ggatctgggg gcaacacatt gccgaatatg ccgatctgcg cattcgcatg ttcccggcga
     9061 aaggatcgct gctgatcatg gatcaccgca ttaaccagca tgtgatcaac cgctgccgta
     9121 aaccttccga cgccgatatt ctggtgcctg gcgataccat ttcgctgatt ggtaccacct
     9181 ctttacgtat tgattacaac gagattgacg ataatcgagt gacggcagaa gaggttgata
     9241 ttctgctgcg tgaaggggaa aaactggccc ccgtgatggc gaaaacgcgc attttgcggg
     9301 cctattctgg cgtgcgcccg ctggttgcca gcgatgacga cccgagcgga cgtaacgtca
     9361 gccgtggcat cgtgctgctc gaccatgctg aacgcgatgg tctggacgga tttatcacca
     9421 tcaccggtgg caaactgatg acctatcggc tgatggctga atgggctacc gacgcggtat
     9481 gccgcaaact gggcaacacg cgcccctgta cgactgccga tctggcactg cctggttcac
     9541 aagaacccgc tgaagttacc ttgcgtaaag tcatctccct gcctgccccg ctgcgcggtt
     9601 ctgcggttta tcgtcatggc gatcgcacgc ctgcctggct gagcgaaggc cgtctacacc
     9661 gtagcctggt atgtgagtgc gaagcggtaa ctgcgggtga agtgcagtac gcggtagaaa
     9721 atttaaacgt taatagcctg ctggatttac gccgtcgtac ccgtgtgggg atgggcacct
     9781 gccagggcga actctgcgcc tgccgcgctg ccggactgct gcaacggttt aacgtcacga
     9841 cgtccgcgca atctatcgag caactttcca ccttccttaa cgaacgctgg aaaggcgtgc
     9901 aacccatcgc ctggggagac gcactgcgcg aaagcgaatt tacccgctgg gtttatcagg
     9961 gactgtgtgg tctggagaag gagcagaaag atgcgctttg atactgtcat tatgggcagc
    10021 ggcctcgccg gattactctg tggcctgcaa ctgcaaaaac acggcctgcg ctgtgccatt
    10081 gtcacgcgtg gtcaaagcgc gttgcatttc tcatccggtt cgctggattt gctgagccat
    10141 ctgccagatg gtcaacccgt ggcagacatt cacagtggac tggaatcttt gcgtcagcag
    10201 gcacctgcac atccttactc tcttctcggg ccacaacgcg tgctcgatct cgcttgccag
    10261 gcgcaggcat taatcgctga aagcggtgcg caattgcagg gcagcgtaga actggctcac
    10321 cagcggatta cgccgctcgg cactttgcgt gcaacctggt taagttcgcc agaagttccc
    10381 gtctggccgc tacccgcgaa gaagatatgt gtagtgggga taagcgggct gatggatttc
    10441 caggcgcatc ttgcggcggc ttcgttgcgc gaactggatc tttcggttga aactgcagaa
    10501 atagagctgc cggaactgga tgtcctgcgc aataacgcca ccgaatttcg cgcggtgaat
    10561 atcgcccgtt tccttgataa taaagaaaac tggccgctgt tacttgatgc gcttattcct
    10621 gtcgccaata cctgcgaaat gatcctgatg cccgcctgct tcggcctagc cgatgacaaa
    10681 ctgtggcgtt ggttgaatga aaaactacct tgttcactga tgcttttgcc aacgctgccg
    10741 ccttccgtgc tgggcattcg tctgcaaaac cagttacagc gccagtttgt gcgccagggt
    10801 ggagtgtgga tgccgggcga tgaagtgaaa aaagtgacct gtaaaaatgg cgtagtgaat
    10861 gaaatctgga cccgcaatca cgcagatatt ccgctacgtc cacgtttcgc ggttctcgcc
    10921 agcggcagtt tctttagtgg cggactggta gcggaacgta acggcattcg cgagccgatt
    10981 ctcggccttg atgtgctaca aaccgccacg cggggtgaat ggtataaggg cgattttttt
    11041 gcgccgcaac cgtggcagca gttcggtgta accactgatg agacgctacg cccgtcgcag
    11101 gcagggcaaa ccattgagaa cctgtttgcc atcggttcgg tgctgggcgg atttgatccc
    11161 atcgcccagg gatgcggcgg cggtgtttgt gccgtcagtg ctttacatgc cgctcaacag
    11221 attacccaac gcgcaggagg ccaacaatga acgacaccag cttcgaaaac tgcattaagt
    11281 gcaccgtctg caccaccgcc tgcccggtga gccgggtgaa tcccggttat ccagggccaa
    11341 aacaagccgg gccggatggc gagcgtctgc gtttgaaaga tggcgcactg tatgacgagg
    11401 cgctgaaata ttgcatcaac tgcaaacgtt gtgaagtcgc ctgcccgtcc gatgtgaaga
    11461 ttggcgatat tatccagcgc gcgcgggcga aatatgacac cacgcgcccg tcgctgcgta
    11521 attttgtgtt gagtcatacc gacctgatgg gtagcgtttc cacgccgttc gcaccaatcg
    11581 tcaacaccgc tacctcgctg aaaccggtgc ggcagctgct tgatgcggcg ttaaaaatcg
    11641 atcatcgtcg cacgctaccg aaatactcct tcggcacgtt ccgtcgctgg tatcgcagca
    11701 tagcggctca gcaagcacaa tataaagacc aggtcgcttt ctttcacggc tgcttcgtta
    11761 actacaacca tccgcagtta ggtaaagatt taattaaagt gctcaacgca atgggtaccg
    11821 gtgtacaact gctcagcaaa gaaaaatgct gcggcgtacc gctaatcgcc aacggcttta
    11881 ccgataaagc acgcaaacag gcaattacga atgtagagtc gatccgcgaa gctgtgggag
    11941 taaaaggcat tccggtgatt gccacctcct caacctgtac atttgccctg cgcgacgaat
    12001 acccggaagt gctgaatgtc gacaacaaag gcttgcgcga tcatatcgaa ctggcaaccc
    12061 gctggctgtg gcgcaagctg gacgaaggca aaacgttacc gctgaaaccg ctgccgctga
    12121 aagtggttta tcacactccg tgccatatgg aaaaaatggg ctggacgctc tacaccctgg
    12181 agctgttgcg taacatcccg gggcttgagt taacggtgct ggattcccag tgctgcggta
    12241 ttgcgggtac ttacggtttt aaaaaagaga actaccccac ctcacaagcc atcggcgcac
    12301 cactgttccg ccagatagaa gaaagcggcg cagatctggt ggtcaccgac tgcgaaacct
    12361 gtaaatggca gattgagatg tccacaagtc ttcgctgcga acatccgatt acgctactgg
    12421 cccaggcgct ggcttaaact cccttctgat gcccggtaag catgtggtta ccgggcattt
    12481 ttgcgtacac gattccgtgc ccaatgtatg cgttgcaacg cagtgaaaat tcctctgaaa
    12541 acgtctcgca aaggctgaaa ctggcagatg tcaaaggcct gagataaccg taatgtcgcg
    12601 tcatcataaa tatcaggtga cggacaacca tgaccgaatc aacaacctcc tccccgcatg
    12661 atgcggtatt taaaaccttt atattcacac ccgaaaccgc acgggatttt ctcgaaatac
    12721 atttaccaga accactgcgc aagctttgca acctgcaaac cttacgcctg gaacccacta
    12781 gttttattga aaaaagttta cgcgcttact actcggatgt tttgtggtcc gtggaaacca
    12841 gcgacggtga cggctatatc tactgcgtga ttgaacatca aagctctgca gaaaagaata
    12901 tggcttttcg gccaatgcgc tatgccactg ccgccatgca gcgtcaccta gataaaggct
    12961 atgacagagt tccgctggtg gtgccattgc tgttttatca tggcgaaaca tcgccctacc
    13021 cgtactcact taactggctg gatgagtctg acgatccgca acttgcccgg cagttgtaca
    13081 ccgaagcttt tccgttggtg gatatcacca tcgtacctga cgatgagatc atgcaacatc
    13141 ggcgtatagc tctgctggaa ctgattcaaa agcatattcg cgaccacgat ttaatcggca
    13201 tggtcgacag gatcaccacg cttttggtta gaggcttcac taatgacagc cagctacaaa
    13261 cactgtttaa ttatctgctg caatgcggcg atacctcccg tttcacccgt tttattcagg
    13321 agattgccga acgttcacca ctacaaaagg agagattaat gactattgct gaacggctac
    13381 ggcaggaagg gcatcaaatt ggctggcagg aaggtatgca tgaacaagcc attaaaattg
    13441 ccttgcgcat gctggagcag ggctttgaac gtgagattgt gctggcggca acccaactca
    13501 ctgatgctga tattccgaac tgttattaaa aggagaggga gagattgatg actattgctg
    13561 aacggctacg gcaggaagga catcaaattg gctggcaaga aggtaaatta gtaggtttgc
    13621 agcaaggtaa attagaaggt ttgcagcaag gtatgcatga acaagccatt aaaattgctt
    13681 tgcgcatgct ggaacagggc attgatcgtg accaagtgct cgcggccacc cagctaagcg
    13741 aagccgatct ggcagcgaat aaccactaat taacacaggc ccacagccga tccccatggg
    13801 cctttgatat caataactac cttttatgcg tggcccattt ttgcctgatt taaacatcgc
    13861 cagtcgttga tccagggcat cgctgtagag catcgtgtca acgccaacag cgacaaagtt
    13921 cgctccccac gccaggcatt gctgcgccat atcaggagcc acagccagaa aaccagccgc
    13981 tttacccgca gcacggatcc gccgaatgct ggtttcaata attcgctgca cttccgggtg
    14041 cccggcgtta tccgggtagc ccaacgacgc agaaagatcc gctggtccaa taaacacgcc
    14101 atcaatccct tcgacgtcga ggatctcgtc caggttatcc agtgccgttt tactttccac
    14161 ctgcactaac agacaaagcg aatcgttgac ttgcgccatg taattctcaa tgcgtcccca
    14221 gcgcgcagcc cgtgccacac tggccccaac accacgctca ccgtagggag ggttggacaa
    14281 attttgccgg agggggaatg tgccggtgca gtaaaccggc acagagtggc gaaatcagac
    14341 ggataaagtc atggactcaa caacctcaat ccagccatgc tcactagcga tgtcctggcc
    14401 gtttaaccaa cggcgtagca tattcagtgc catcattgcg cacacttcct gacggatagc
    14461 caggctgtag cgtgtagtgc tgaaactcac acgcagagcg aaagtgccgt ctggcgtggc
    14521 tagcgcaaag ttgagatgct cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa
    14581 atggttggcc cgccgttctg taatccagtg cgcagtttgc gccagggttt cttcctgtga
    14641 aggaatcact tcacacgcca gcaatggagc acctgcgcga gaaagttgca aagccaataa
    14701 accaccggtg aattgctcgc tcaacgtcag gctgaactgg cggttttgca attcgcgact
    14761 gatctgcgcg ggcagtcctt cggtgccttc gaaaatcacg ctctgtccgg caacacgttt
    14821 aacgtccagc cacagttttt ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag
    14881 ttcgatgatc ggcattgagg agcgatagcc cattgttacg cccggcggca gttgtagagt
    14941 gtccaggctt tgtgccagat cgctttccga acgaccaaag gtagttaaac gcagacaaac
    15001 cggcggctgt ggtaaagaaa aacgctcgcg caggcgcggc aggatttcgt gctcgaccat
    15061 caccttaaat tctgacggta cgccgggagt aaagaacatc aggcaacgat taagctgcac
    15121 ggcaaaacca caggcggtgc ctaccgggtt attgataaat tcagcactgg caggcagctc
    15181 cgcttgttta cggttgctcg gtgccattac tcgtccacgt tcgtggaaat agcgttccat
    15241 ctctttgagc caggcttcat gcagcacaag gccttcacct tttgctgtcg cagcggcgag
    15301 tgcgcttaaa tcatcgctgg tcggtcccag cccgccgtta acgatcagca catcggcgtg
    15361 ctgactacgt tcgcgaagaa tggtgactaa gtcatcaagg ttatccccca ccgtattgcg
    15421 gcgagataat ggcagcccct gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc
    15481 aacgatttgc ccgtataaca cttcatcccc ggtggatagc atttccactt ttaacatggt
    15541 tatctcctgc ttttgttatc gaatcactat aacgcaaagg gagataaggc gtagtaagaa
    15601 actgaatgcc gcgccggaag ggatcagaaa ctggcgttaa ccccaacata caggccgtca
    15661 gccacagcgt tgtcgcggtt accgtcttta cccgacagat tcaggtagcg ataacccgcc
    15721 tcaatactga ctggacgcat aatggtgtaa cgcgcgccag cattcgcttc ctcataactt
    15781 tgaataccgc tggagagcga atccggagag tagtaatact cgccaaacaa acggaagctg
    15841 ttgccaattt tccactgcaa accacctcct accgctgcgg cataaccttc atcgccgtaa
    15901 ttcgggttgg tgtacacgcc ttttccgcca acggtcgcca ttaacggccc gagaggcaga
    15961 ttcaacccca gccccacgcc cgcgacgtca ccgtcgtcgt cgttatgtgt ccagttaccg
    16021 cttaaagcca ggcccgtcga ttcagtccca aaaccgacac caatattggt gtattcttta
    16081 cccgcctggc cgctgatgct cattgcattg accgatgcag aaaccaacag cattccggcc
    16141 agacctgcta gcgcaatttt tttcattatc ctctttccca caagcaatta atcaaaattc
    16201 ccaaagctgc gggattgtac tgcctggcac agaggattca atgagcgaaa aaagggatct
    16261 agtcacttat tgtaactaaa tacttcttaa ataattaaac gccgaaaaaa taaacacact
    16321 ttaccgatac acacaaaaat aataataagt gcagttatca ttgcaaaaga gattactgtc
    16381 actttcacat atttctgagt taccgtattc tcatggtttc tttttattta taaggaatac
    16441 ccctgtgcga caacggacta ttgtatgccc tttgattcaa aatgatggtg cttatttgct
    16501 gtgcaaaatg gccgacgatc gcggcgtttt ccccggtcaa tgggcgcttt cgggtggcgg
    16561 cgtggagtct ggcgaacgaa ttgaagaggc actacgccgc gaaattcgcg aagaactggg
    16621 agaacagctg cttttgacag aaatcacgcc gtggaccttc agcgatgata ttcgcaccaa
    16681 gacatatgca gatggtcgca aggaagagat ttatatgatt tacctgattt ttgactgcgt
    16741 ttctgccaac cgagaagtga aaataaacga agagtttcag gactacgcgt gggtaaaacc
    16801 tgaagatctg gtgcattatg atttaaatgt tgccacccga aaaacgttac gtttgaaagg
    16861 tcttctttaa caatgatgac agccatcact ggctgtcaga ttgaaaattt agtggttaac
    16921 gaattcccca tccagataaa ctttgccttt ttccacatgc atgactaaac catccagata
    16981 atcaggttta aatatcgcat cacgcttatt tttagcaata tatgtcaggc aatgattatg
    17041 ggtgaaaata acgatatttt tatcaggcgc tttgctttgt aagtccttaa ttgcactata
    17101 aatctcatta ccgcactgaa gaagtcgttt atctaccgtc agctttttac ccgcggaaaa
    17161 ccaggtcgcc gactgaatgg tccggacggt attactggaa taaagatcga aatcagggat
    17221 gtcagcgcta aaagcgttgc ccagttcacg ggcatcctgg gtacctttaa ccgtaatacc
    17281 tgttttatct gacaagcatt gattggttga acgatcgcaa cgttcagcat ggcgaaacaa
    17341 aacgacaacc gggtgctgct gtgccagtct ggccaatgtt ttattgtcga tacgtggcaa
    17401 accattgcta ctccaggcgg catgagtacc cagtccggca attgcagcga gcgccagtaa
    17461 aatgatgaaa tatttttttg acttcaatga tgagcggcaa aaagctaaca cgtaaactcc
    17521 acctgtagac aagcgcaacc agacaattac cgtgaaattg agcgacattt ctggcgataa
    17581 ttagcagttg gtgtaatatt aaaaatccta cgatgtcggc aaaatgcctc aaatttttgc
    17641 caaatgcaaa gcctaaataa gaaaaaatat aaaaatttca atatttacgt ctaatattag
    17701 tttcttaagg ttaagttaat attctatcct taaaatttcg ctccaaatgg caaaatatac
    17761 acaacactct ttatagcaaa tataagtgga caggtattca atggcggaag gaaaagcaat
    17821 gtcagaattt ttgccttttt cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa
    17881 agaggttctc gaatccggtt ggatcacaac cggtccgaag aatcaggcgc ttgagcaagc
    17941 cttttgccag ttgacgggaa atcagcatgc catcgcggtc agttcagcca ccgccggaat
    18001 gcatatcacg ctaatggcgt tggaaattgg caagggcgat gaagtgatta cgccttccct
    18061 gacctgggtt tcaaccctca atatgatttc cttgctgggt gcaacgccgg taatggtgga
    18121 tgtcgaccgc gatacgctga tggtcacgcc tgaagcgatc gaatcagcca ttacgccacg
    18181 cactaaagcc atcattccgg tgcattatgc cggtgcgcca gcagatattg acaccattcg
    18241 cgccattggc gaacgttacg gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac
    18301 gtattacaaa gggcgacata ttggcgcaaa aggtaccgct attttttcat ttcatgccat
    18361 taaaaatatt acctgtgctg aaggtggcct gattgtaact gataatgaaa accttgcccg
    18421 ccagctacgg atgctgaaat ttcacggtct gggtgtcgat gcctatgaca gacaaacctg
    18481 gggccgtgca ccgcaggctg aagtcttaac accgggctat aagtacaatc tgaccgatat
    18541 taacgccgcg attgccctga cacagttagt caaattagag cacctcaaca cccgtcggcg
    18601 cgaaattgcc cagcaatatc agcaagcact ggcagctctc ccctttcagc cattaagcct
    18661 tcccgcctgg ccgcacgttc acgcctggca tctgtttatt attcgtgtcg atgaacaacg
    18721 ttgtggtatc agtcgcgatg cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg
    18781 gttacatttc cgcgccgctc acacacaaaa atattatcgc gagcgttttc ccacgctgtc
    18841 gttaccgaat accgaatgga atagcgaacg catctgttcg ttgccgctgt tcccggatat
    18901 gactaccgcc gatgccgacc gcgtcatcac agcccttcag caactcgcag gacaataagc
    18961 catgtttgaa atccaccctg ttaagaaagt ctcggtggtt attcccgttt ataacgagca
    19021 ggaaagctta ccggaattaa tcaggcgcac caccacagcc tgtgaatcgt tggggaaaga
    19081 gtatgagatc ctgctgattg atgacggcag tagcgataat tccgcacata tgctggtcga
    19141 agcctcacaa gcggagaaca gccatattgt gtctattttg cttaaccgca attacgggca
    19201 acattcagcg attatggcgg gattcagtca cgttactggc gacttaatta ttacccttga
    19261 tgccgatctc cagaatccgc cagaagaaat cccccgcctg gtggcaaaag ccgatgaagg
    19321 ttacgacgtg gtagggactg tacgccagaa ccgccaggac agctggtttc gtaaaacagc
    19381 ttcgaagatg attaaccggc ttattcagcg caccactggc aaagcgatgg gtgactacgg
    19441 ttgtatgctg cgcgcctatc gccgtcatat tgtcgatgcg atgttgcact gccatgaacg
    19501 cagcaccttt atcccgattc tggcgaatat cttcgcccgc cgtgcaattg aaattccagt
    19561 acatcatgcc gagcgtgagt ttggtgaatc caaatacagt tttatgcgcc tgattaattt
    19621 gatgtacgac ctggtgacct gccttaccac aacgccgcta cgtatgctga gtctgctcgg
    19681 cagcattatt gcgattggag gttttagcat tgcggtgctg ctggtgattt tacgcctgac
    19741 cttcggacca caatgggcgg cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt
    19801 tattggcgct cagtttatcg gcatgggatt actcggtgaa tatatcggca ggatctacac
    19861 cgatgtccgc gcccgccccc gctattttgt tcagcaagtt atccgtccat ccagcaagga
    19921 aaatgaataa tgaaaaccgt cgtttttgcc taccacgata tgggatgcct cggtattgaa
    19981 gctctgctgg ctgccggtta cgaaattagc gccattttta cccataccga taatcccggt
    20041 gaaaaagcct tttatgattc ggtggctcat ctggcggcgg aaagagacat tccggtttat
    20101 gcgccggata acgttaatca tccgctgtgg gtggaacgca ttgcccaact gtcaccagag
    20161 gtgattttct ctttttatta tcgccatctt atttgcgacg agatttttca gctcgctccc
    20221 gcaggtgcat ttaatctgca cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg
    20281 aactgggtgc tggtgaacgg tgaaacggaa acgggcgtta cattgcaccg aatggtaaaa
    20341 cgtgccgatg ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc
    20401 gctattacgc tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta
    20461 cccgccatta aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt
    20521 tttggtcgca gaacgccgga tgacagtttc cttgaatggc ataaaccggc atccgtactg
    20581 cacaacatgg tacgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat
    20641 cagaaattca ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg
    20701 agcgtgattt ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc
    20761 accggacagg cgggcgacgg cattactatg cagggctcgc aattagcgca gatgctgggc
    20821 ctggtgcaag gttcacgctt gaatagccag cctgcctgca ccgcccgacg ccgtacccgg
    20881 gtactcatcc tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc
    20941 gaagatcatt atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg
    21001 aatcatccac attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag
    21061 tatcacgtca aaaaatgtga tgtcgtcttg ccgctggtgg cgatagccac gccgattgaa
    21121 tatacccgca acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc
    21181 cgctactgcg ttaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg
    21241 atgtgtagcg ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat
    21301 aaaccacgct ggatttattc ggtgtcaaaa caattacttg atcgggtgat ctgggcctat
    21361 ggcgaaaaag agggtttaca gttcaccctc ttccgcccgt ttaactggat gggaccacga
    21421 ctggataacc ttaatgcagc gcgaattggc agctcccgcg ctattacgca actcattctc
    21481 aatctggtag aaggttcacc aattaagcta attgatggcg gaaaacaaaa acgctgcttt
    21541 actgatatcc gggacggtat cgaggcgttg taccgcatta tcgaaaatgc gggaaatcgc
    21601 tgcgacggcg aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg
    21661 ggcgagatgc tgctggcgag cttcgaaaaa catccgctgc gccatcattt cccaccgttt
    21721 gcgggctttc gcgttgtcga aagtagcagc tactacggca aaggatatca ggacgtagag
    21781 catcgtaaac cgagcatccg caatgcccac cgctgcctgg actgggagcc gaaaattgat
    21841 atgcaggaaa ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat
    21901 aaaccatcat gaccaaagta ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg
    21961 aaggcgtgcc gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt
    22021 tcagcgtcgg cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt
    22081 tgtggaagat gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag
    22141 gtacggcctg gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg
    22201 ctaaacatca cgaagtcggt ctgcacgcct gggatcacca tgcctggcaa gcccgtagcg
    22261 gtaactggga tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag
    22321 agattatcgg tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg
    22381 tgatcgaagc aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatgc
    22441 cgttccgtcc actgctcgaa tcaggaaacc ctggcactgc gcaaattccg gtgaccttac
    22501 ccacctggga tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac
    22561 tcaaccgcat cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaag
    22621 gctgcgctta tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga
    22681 cattttgccc tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg
    22741 gaaatattgc cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat
    22801 gaaatcggta cgttacctta tcggcatctt cgcgtttatt gcctgctatt acctgttacc
    22861 gatcagcacg cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa tcagtcgaga
    22921 aatgctggca tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa
    22981 acccattgcc ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt
    23041 tggtgtgcgg gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt
    23101 tactctgcgc ttatggcgcg ataaacgtct ggctctactc gccacagtaa tttatctctc
    23161 attgtctatg ccatcggcac ttatgccgtg ctcgatccgt ttattgcatt ctggctggtg
    23221 gcgggaatgt gcagcttctg gctggcaatg caggcacaga cgtggaaagg caaaagcgca
    23281 ggatttttac tgctgggaat cacctgcggc atgggggtga tgaccaaagg ttttctcgcc
    23341 cttgccgtgc cggtattaag cgtgctgcca tgggtagcga cgcaaaaacg ctggaaagat
    23401 ctctttattt acggctggct ggcggttatc agttgcgtac tgacggttct cccctgggga
    23461 ctggcgatag cgcagcggga gcctgacttg tggcactatt ttttctgggt tgagcatatt
    23521 caacgctttg cactagatga tgcccaacat agagctccgt tctggtacta cgtgccggtc
    23581 atcattgccg gtagcctgcc gtggctggga ttactccccg gtgcactgta cacaggctgg
    23641 aaaaaccgca agcattccgc aaccgtctat ttgttgagct ggacgataat gccgctgctg
    23701 tttttctccg tcgctaaagg caaattgccc acctatattc tttcttgctt tgcacctctg
    23761 gcaatgctgt tggcgcatta cgctttgctg gcagcaaaaa ataatcctct ggcgctgcgg
    23821 attaatggct ggattaacat cgcttttggc gtcactggca ttattgccac atttgtgatc
    23881 tccccctggg gaccaatgaa cacgccggtg tggcaaacct tcgagagcta taaagtcttt
    23941 tgtgcctggt cgattttttc gctatgggca tttttcggct ggtacacctt aacaaacgtc
    24001 gaaaagacct ggtctttcgc cgcgctttgc ccgctggggc tggcgttgct ggtaggattt
    24061 tcaattcctg acagagtcat ggaaggaaaa catccgcaat tttttgtcga gatgacacaa
    24121 gaatcacttc agccaagccg ctatattctt actgatagcg tcggtgttgc cgcaggtctg
    24181 gcatggagcc tgcaacgcga tgacatcatc atgtatcgcc agacaggtga gttgaaatac
    24241 ggccttaatt atccggatgc gaaagggaga tttgtcagcg gtgatgagtt cgcaaactgg
    24301 cttaatcaac atcgtcagga ggggattatt acactcgtgc tttcggttga ccgcgatgaa
    24361 gatatcaaca gtctcgccat tccgcccgca gatgccatcg ttcgtcagga gcgtctggtg
    24421 ctgattcagt atcgtcccaa atgatctggc taacattagt ctttgccagc ttgcttagcg
    24481 ttgccgggca gttgtgtcag aaacaggcaa cctgctttgt ggcgataaac aaacggcgca
    24541 aacatatcgt gctgtggctg ggactggcgc tggcttgtct tggtcttgcc atggtgctct
    24601 ggctgctggt cttgcagaac gtaccggtag gcattgccta cccgatgtta agtctgaatt
    24661 ttgtctgggt gacgctggct gcagtaaaac tgtggcacga accggtatcg ccgcgtcact
    24721 ggtgtggggt ggcgttcatt attggtggca ttgtgatcct cgggagtacg gtgtaatggg
    24781 cctgatatgg ggattattca gcgtcattat tgcctcagtt gcgcaattaa gcctgggttt
    24841 tgcggcaagt catctgccgc cgatgacgca cttatgggat tttatcgcgg ctctgttagc
    24901 ttttggcctg gatgcccgga tactgttact cgggctgctg ggttatctgc tttccgtttt
    24961 ttgttggtat aaaacgctac ataaacttgc gctaagcaaa gcctatgcct tgctcagtat
    25021 gagttatgtg ctggtatgga ttgcttcaat ggtacttccc ggctgggaag ggactttttc
    25081 gctgaaagca ctactgggag tagcctgtat tatgagcggg ttgatgctga ttttcctgcc
    25141 cacgacaaaa caacgttact gagttttccc tgccgctgta cactgccgtt cccactcatc
    25201 ggggctataa ctgcttgccg agaggacttt caccgtatgc agcttttcac gattaataca
    25261 ataaagcgca ttctgcaaag gcgagagtaa atcccccact ttcacggcga agtcactctt
    25321 cacttcggcg atcattttta tcgcgccgcc agcatcgcag agcataagta catgtctgtt
    25381 atcttgttta ttgcagcacg attttttgtg ccagccattc cattgcatta tcctgtttgc
    25441 taagagtttt cacatcaatt tacagattaa cagataaact ctttatcaac ggcaatggaa
    25501 tggcagaaat gagaagtgaa acggagggtt aattattgct gacgttgcac ccactctttt
    25561 agcgcctgac gtgaaatttt aataccgccg tttttcagct ccggcggtaa agtcagccag
    25621 cgcaccggtt gttgaaaacg agccagctta tctttcaccc attcgccaag atcaacggtt
    25681 tgctggtcat actccaccac cgctaccggc cgatgaccaa actccttgtc ggcaacggaa
    25741 acgataaaca cctgcaaaac cgcaggatgt gcagcaataa cacgctcgac ttcttccggc
    25801 tgaataccct caccgccact gaagaacaga ttgtctaaac gtccgacaat ggtcagcttg
    25861 ccattatgca tctcaccgcg atcgcgcgta gcgtaccagc cttcgtcatt aaccagtgaa
    25921 accagttgcc cgttacgcca gtaaccttct gccatactgg cagcccgcag ccacacttca
    25981 ttattaacga ttttcacttc ccgacccggc agcggcaaac caacgtctgc caggccgtcg
    26041 gcttctttcg cacataccgt ggaggcaaac tcggtcagac catagccgca aaagcaacga
    26101 atcccctgct cgcgcgcctg ttccgtcaac tcgaccggga tagccgcgcc gccaagtaac
    26161 accgctttca gggaaacgga actgcggtta accagcaaac gccagagttg tgttggcacc
    26221 agtgaagcgt gagtacagcc tgccagcatt tgctccaatg gctgtttatc acgcaccgtc
    26281 atccgcgcac cagcgtataa ccagcgccac ataattccct gaccggagac gtgaaacagc
    26341 ggtaaagaga gcaaccagtc atcgtgatcg ccaaacggaa tcagcgataa cacaccttgc
    26401 gcactggcaa gatgggcctg ataagtatgg acagcggctt tcggcaaacc ggtagaaccc
    26461 gaggtcaacg tcattgagca cagacgcgtc ggctgccacg cagcggcatg tacgccttca
    26521 accagctgaa tgtgcagcga cgttaatgcc ggaaacgtgt tttccccatc cggcaccagc
    26581 gcaaattgca gcgtcagatt gggcagcaat tcttcaagca acggttgcgg cagctgaggg
    26641 ttcacgggca acacccgcgc cccgcattgc agtaacgcca gccaggcgag cagcgtttgc
    26701 ggcgtattcc acgcccgcaa catcacgccg ctgccctcaa ccacaccctg cgccgcaaag
    26761 cccgtagcta actcatcgac gcgagcacaa agctcgcgcc agttgagttg ctcgtcatta
    26821 agacgtaagg cgatggcttc tccccgcact tgccgccagt gacgccacgg ccagtcagag
    26881 aagatcataa caactgctcc agtacatcag cgtccactaa cggcagtggg ctccccggcc
    26941 agcgacgaac ctgctgcgcc tgcatcagat ccagcgtgtc cagtcccgga atggtgtctg
    27001 gcgttaacca ggcagcaatc cgcgccagtt gcgttagacc taagctcgac tcaatggaag
    27061 aactgatcac cgccgtcagc cccagcgcat gcgccgcctg tacctgctcg cgtacttttt
    27121 ccagactgcc cgtgagcgtg ggtttgataa ctaccgcgcg cacgccctct tcagccacaa
    27181 aggcaaaatc cggctcgcgc aggctttcat cccaggcaat ggcaatgccg gtttcacggg
    27241 caaacgctcg cgaatcatcg cgggttttgc acggctcttc gagaaacgcg atgcggtggc
    27301 gataatccgg gttaacatat ttggcaaact gctgaccttt cagcggtgtc caggcgcgat
    27361 ttgcgtcaag acgcaaatgc agatccggaa ttgcctccag caacagattc accaccatgc
    27421 cgtcgcgcac cgcttcgtac aatcccactt tgaccttcgc cactttctcg cccggcatat
    27481 cggcaagttt gaggatcaga tcgtccggat cgccattaca cagcggtgcc gcacggtagt
    27541 tggctgcttg cggcaacgta tctgtcagtt ctgccaatgc acagcttacg ccaaaggcca
    27601 cggaaggcat ctgcggtagc tcgcaatcgc ctgccagcca gttatttacc caggcaagca
    27661 gcacgctttg cgcctcttcc cagttttcct ggctgaagcc cggcagtggg gagatctccc
    27721 cccacccttc gcgctcgccc tcacgcaggc aaacgtacag cccgtcgcgg gtttttaacc
    27781 gcctgtcgcg cagaaccacc cccgcgtcca tggggatctg ccagcggtat acctgcgcgc
    27841 tacgcattac ggattccgtt taaatttgct gaaatcaggc tgacgtttct ggttgaaagc
    27901 gttgcgacct tcctgacctt cttccgtcat gtagaacagc atggtggcgt tgcccgccag
    27961 ctcctgcagc cccgcctgcc cgtcacagtc ggcgttcagc gcagctttca ggcagcgcag
    28021 cgccatcggg ctgttttgca gcatttcgcg gcaccaacgg acggtttctt tttccagatc
    28081 cgccagcggt accacggtgt tgacaaggcc catatccagc gcctgttttg cgtcgtactg
    28141 acggcacagg aaccagattt ctcgcgcttt tttctgcccg acgatacgag ccatgtagga
    28201 agcgccccag ccgccgtcga aggaaccgac tttcgggcca gtctgaccga agatggcatt
    28261 atctgctgcg atagtcaggt cgcacatcat gtgcagaacg tgaccaccgc cgatggagta
    28321 gccagccacc atcgcgacaa ccggtttcgg acaggtacgg atctgacgct ggaagtccag
    28381 cacgttcagg tgatgtacgc cggaatcatc tttatagccg ccgtaatcac cacgcacttt
    28441 ctggtcacca ccggagcaga acgctttatc gcctgcgcca gtcagaatga tcacgccgat
    28501 attgtcgtca taacgcgcat ctgccagtgc ctggatcatc tcttttaccg tcagaggacg
    28561 gaaggcattg cgcacctgcg gacgattaat ggtgattttt gcgataccgt cggtggattt
    28621 ttcataacga atgtcttcga aaccttcgga gcagtcgtgc cattcaaccg gtgcgtaagg
    28681 cattgcttca tcaggataaa tcatattgtg tcctttagtc aaaaacgcaa gatctgcgcc
    28741 agacttgcga ttacgccagc gggattttcc cgatgcgcgt tatgtccggc gcgaggaatg
    28801 acatggcagt cggcagccag ttccgccgcc agggcgcgga atttgctgtc acgttcacca
    28861 cataaataat aaaacgcaaa tgtgcgggcg ctaaggttag cacgtaaatc aggctggacg
    28921 gcgagagaag tcgcctccag catggcggca agggttgcgc cattattgtt gctgcgcagc
    28981 gccaccagct cccggcgttg atcgtcattg agcgaggcaa aaacaggctg ttgataccag
    29041 tcggcaaata ccgccgttaa cggttctgtg cgaaaacgct gcgcccattg gcgatcggaa
    29101 cgctgacgtt ccgcacgttg ttcagcattt tgcagccccg gatgcccgcc ttcgataata
    29161 accccacaaa gccccgccac cccctggcaa gccgccatca tcgccacccg tccgccaagc
    29221 gagtacccca ccagccagaa gtcaaggatg ttgtaactaa ccaaggtttt acgcagtaag
    29281 tcggtgacat catcaaatcc atcgacgcta atcgccgccg aaccaccatg acctgggaga
    29341 tcaacataca accgtgagta gtcggcaaac gcctcgccca cttcttgcca ttcgtggcaa
    29401 tcgccggaaa aaccgtgcaa aaacaccagc cagggtaaac ctggttttcc gtgttttgcc
    29461 tgcgcgtgca ggatcataaa tggcttacct gcgccagaag ttgctggagc gtttgcgcac
    29521 catcggtgtc gttaaccacc atttcaatca ccgtggtggt tggcgtgcgc caggcgtcgg
    29581 caaatgccgt ttcaagttcc tgccagtttt gcggacgatg atatttcagc tcgaacatcg
    29641 cggcggcgtg ctcaaaatgg acgttttgcg gcatcagata gaaacgctca cgctcgcttt
    29701 gcggcgttgg caacagcgag aaaatttgcc cgccgttgtt gttcaccaca attaatacca
    29761 gcggcgcaga aacctgacgc aataacgcca gcgcgttgag atcgtaaagt gcggagagat
    29821 cgcccacaat cgccagcgtc ggtttgccgc ttgcccgctg aacgcctgcg gcggtcgaaa
    29881 gcagtccgtc gataccgctg gcccctcggt tgctgtacac cgggtaacct gccggaagtt
    29941 gcgaaagcgc atcaatcaga cgtaccacca ggctgttacc aacaaacaat tgcccctgtt
    30001 caggcagata gtcgctgatg cgatgcgcca gttgcgcttc gccaaacgca tcacggcggg
    30061 caataaccgc ctgcattgcc tgttccgcca ggcgcgggat ttcaacgcac cagggctggc
    30121 gtttttctgc cggatgcagc tccagccagt cggcaatatt gccaattaag cgacgtccgc
    30181 gatggtgtgc cggatcaagt cgcccttcaa tgtcatcaac aatccagtac tcttctggtt
    30241 cacagcttgc ctgccattgc aggagccgtt tccccgtcag gctgcttccc agttgcacca
    30301 caatttgtgc ctgctgcagc tcgctggtcg ctttggcatt gcctaaccaa agatcggcac
    30361 acggcagcgg ctgcccggtt tgtgacagca catcgccaat cagcggccag ccaagagttt
    30421 gcgcccacag ggcaactttt ttgccctctt ccgcactcat gcgcccggca accaccacgc
    30481 cgcgcttttg tcgccagaag aaccagtcgc gctgtttttc actttccaga cgaggcgctt
    30541 cacgcagcca cggtttgtcg tcctgccacc agtcacccag acgctgttgc cagctaagcc
    30601 cggtatcgtc catttcgcca tacagcggtt cagcaaacgg gcagttgata tggactcccc
    30661 ccgcatgaag cgtaccgaga gcgtggtcga tggtagaaac cagccaacgt gcggggatat
    30721 cctgggtcgg gcgcggcaat gaaatactgt gcgtggggtg agaggcgaac attcccggct
    30781 ggcgaattgc ctgattcgcg ccgcagtcaa ttagctccgg cgggcgatcg gcggttaaga
    30841 gaatcagttt ttctccggtt aacccggctt caatcagtgc cggatagaga tttgccaccg
    30901 ccgtgccgga ggtcacaatc accgccaccg gctgcttgct gactttcgcc agccccagcg
    30961 ccagatgccc caacccacgc tcatcgaaat gggtgtggtg aatgaatgcg gaattctccg
    31021 ccgccgctaa cgttaacggt gtagaacgcg agcctggggc gatacagatg tgtctgacgc
    31081 cgtgacgcgt taatgcttcc agaatgaccg ccgcccagcg tcggttaaat gcgcttactg
    31141 acatgagttt gtccggtatc aatattgcgg ctaagtataa ggagctacaa aaatagatta
    31201 ttgatatgaa tcggtaatga tgcgactcat tactattcca tttgtaataa agtacgcagc
    31261 cctgccgctt tgttgtcgat ttcctgccac tcttgctcgg ggtcggaacc acggacaatg
    31321 cccgcgccag catataatcg cacgacattg ccgctaattt ttgctgagcg cagggaaacg
    31381 cagaattcgc tttgttgtaa tgagagatag cccgcagaac cggcgtacca ttctcgggtg
    31441 aacggttcgt gacgggcgat aaactgtcgc gccagatcgc gcggtaagcc agcaactgct
    31501 gccgttggct gcaactgatg taaacagatc acatcatccg ctttgttgag tgaagtccag
    31561 atacagcggc gaagatgctg cactttacgc agacgcagta cctgcggcgg taaaacatcc
    31621 agcgtctggg tatcggcctg taatcgttgg cagatatctt ccaccaccag catgttctcg
    31681 cgctggtttt tatcatccgc catcagcctc tctcctaact gctgcgcctg cttatcatca
    31741 ggatgatttg ctactgttcc cgccagcgct tcagtacgca gcgctttgtc acgccgccgc
    31801 cataaccgtt ccggtgaaga gccaagaaaa gcattttcgc catcaaaggc catgtaaaaa
    31861 tggtagcaat tcagattcag tcgacgactg gcagccatca tcgccgccgc gttgaccgga
    31921 cttgcgaaat gcaggtcagt tgcccgagcg agcaccactt tgtcgagctc acctttggcg
    31981 atggttttcg ttgccagttc gattaattgc gtccagcccg ttttgtccgg ccagtgttgt
    32041 tctcgcgtgg tggttaaatg taacccaggc aagggcttga tactcaccag tgtggcgata
    32101 aattcttttg cctgaatcgc atcgtgctga agggagcttt cgctgaatag cgtcaaccgc
    32161 agcgtggctt taccgccaca gcgtcgccat tccaggcggg gtaaaagtaa attgccctgc
    32221 gacgggtcaa aagcattcag cccccaaatg cgtaagtcgg cgtgttccgg gtgctggcga
    32281 agaaaacgtt gtgcctggtc caacgacgta aaacgggtaa tcgcgcccag gacggcagct
    32341 tcttcatcac cattacgttg ttgccagtag aattgcgggt acgtttgctg actggccagc
    32401 cagctcaagg catcaaaagc gtctttgaga gggaaaggaa tatcgataac ccgaatgccg
    32461 ggtgtcgccg gaatctcttg cgacaaatgg cgcagtagat tttccagcgc cgtagtaagt
    32521 gattgcacgc ggacctctcc ctgttaaaag cctcacatta tacggggtac tacaaaaaaa
    32581 atgcagtacc ccggtgtagg gaggttttaa cggcgtgcca gcaacagtcc tagtaccagc
    32641 ccgacggccg cgcccacacc aattccttgc cagggttttt cgtggacgta gtcatcagca
    32701 cgataaacag cctgcttcgc ccgatagtaa taactgtctg aagcctggct aacccgtttt
    32761 ttcacatcat ccagcgcttt ttctgcacgc gctttcagct caacatattt ctgatcggcg
    32821 ggctcgccag aggagcggag cacctcttcc agtgtttcac taagcagcgt caggtcgtca
    32881 tcgatacgtg tatcaccaaa ctgattagac attctcgttc tccatttgcg taaaacctgt
    32941 cactaactat agacaagagt tgttgattac gcctgaatta cctcgcgtgc catcccaatg
    33001 tgtgggatac aatcttcttc atagacctcc gtcacaggga taaaaccgaa actctggtag
    33061 aagttttgca gatgcgcctg cgccccgagg taaacaggct tatcaggcca gtgatgcgta
    33121 catgtttcga gtgttttact catcagctgc tgccccactt tttcaccacg caacgcttca
    33181 ctgacaatca cccgacctat aacgaccggc tcaagatcat catcactttt cagaatcctc
    33241 gcatacgcca ccagttcatc atttttccaa ccgagaatat ggcgattatc ccccgtcaaa
    33301 tcatcaccat cgatatcctg ataagggcaa ttctgctcga caacaaacac cgcgcaacgc
    33361 agttgtaata aggcataaag ctgggaaacc gataattcag agtgatgcag atcttgccat
    33421 tcaatcataa tgctctcctt ttagatgtat gccttatact agccggaata ttttttgaaa
    33481 cgtgatgaac tcatggaatt aattttttta ggtacttcag ccggtgtgcc aacccgcacg
    33541 cgcaatgtca cggcaatatt gcttaacctg caacatccga cccagagcgg actttggttg
    33601 tttgactgcg gtgaaggcac ccagcatcag ctactgcata ccgcctttaa ccctggaaaa
    33661 ctggacaaga tttttatcag tcaccttcat ggcgatcatc tttttggttt acccggcttg
    33721 ctgtgcagtc gttctatgtc aggcattatc caacccttaa cgatttatgg tccgcaaggt
    33781 atccgtgaat ttgtggaaac cgcgctgcgg attagcggct catggaccga ttatccgctg
    33841 gaaattgtcg aaattggcgc tggcgaaatt ctcgatgatg gcctgcgcaa agtaaccgct
    33901 tatccgctgg aacacccact ggaatgttat ggctatcgta ttgaagaaca tgataaaccg
    33961 ggtgcattaa atgcccaggc attaaaagct gctggcgtgc cgcctggccc actgtttcag
    34021 gaattaaaag cgggcaaaac aatcacgctg gaagatggaa ggcagattaa cggcgcagat
    34081 tacttagctg ctccagtgcc aggtaaagcg ctcgctattt tcggcgatac cggcccctgc
    34141 gatgccgcac ttgacctggc taaaggtgtc gatgtcatgg tgcacgaagc gacgctggat
    34201 ataaccatgg aagccaaagc caatagtcgc ggccatagct ctacacgcca ggctgcgaca
    34261 ctagcccgtg aggctggagt cggcaagcta atcattaccc acgtcagctc gcgctatgat
    34321 gacaaaggtt gtcaacacct gttacgtgaa tgcaggtcaa ttttcccggc gactgaactg
    34381 gcgaatgatt tcaccgtgtt taacgtttaa ctctgcttat tattaaaaac agggtgaaac
    34441 ttgccctgtt atagcaaccc gcgctacata tatccaccca cacaggcata ttgagcgatt
    34501 tttgatattt attcatctcc ttacgcacta tattcagagc aataagttgc ctcttttcat
    34561 cttgattcag cacaaccttt gcaactaaaa ggagcccatg atggttacag ttgtcagcaa
    34621 ttattgtcaa ttatctcaaa cccaactcag tcagacattt gcagaaaaat ttactgtgac
    34681 cgaggaatta ctgcagtctt taaaaaaaac agcgttatcc ggagatgaag aaagtattga
    34741 gttactgcat aatattgcgt taggttatga tgaatttgga aaaaaagctg aagatattct
    34801 ttaccatatt gttagaaacc caacaaatga gacctatcga ttatccgact tattaaaaat
    34861 gcctgtttaa aattatataa tttggcacat accgcaacca actcccacct taaaccaact
    34921 ggtccggata attcagatgt tctcctgttc aaaaagctat tctccccttc gaaattaatg
    34981 acaattatcg gtaatgaaat tcctcttata tctgaaaaac agtcgctttc aaaggtgctt
    35041 ttaaatgatg agaataatga actgagtgat ggtacaaact tctgggataa aaatcgtcaa
    35101 ttaaccacag atgaaatagc ttgctatctt cagaagatcg ccgctaatgc aaaaaatact
    35161 caagtcaatt atcctactgg tctctacgtc ccctactcca ccagaactca cctggaagac
    35221 gctctcaatg aaaatattaa gagcgatcca tcatggccga atgaagtcca gt
//
LOCUS       DADMEQ010000029        34804 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.029, whole genome shotgun sequence.
ACCESSION   DADMEQ010000029 DADMEQ010000000
VERSION     DADMEQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.029"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(18..93)
                     /locus_tag="JNZ05_001474"
     tRNA            complement(18..93)
                     /locus_tag="JNZ05_001474"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="JNZ05_001475"
     tRNA            complement(152..228)
                     /locus_tag="JNZ05_001475"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(331..1716)
                     /locus_tag="JNZ05_001476"
     CDS             complement(331..1716)
                     /locus_tag="JNZ05_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8564754.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYITMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="JNZ05_001477"
     CDS             complement(1907..2647)
                     /gene="rffM"
                     /locus_tag="JNZ05_001477"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HAY8564755.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="JNZ05_001478"
     CDS             complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="JNZ05_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen assembly polymerase"
                     /protein_id="HAY8564756.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="JNZ05_001479"
                     /gene_synonym="wecF"
     CDS             complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="JNZ05_001479"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HAY8564757.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHACEFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="JNZ05_001480"
     CDS             complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="JNZ05_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HAY8564758.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTVMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="JNZ05_001481"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="JNZ05_001481"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HAY8564759.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAEITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="JNZ05_001482"
                     /gene_synonym="wecD"
     CDS             complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="JNZ05_001482"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HAY8564760.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="JNZ05_001483"
     CDS             complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="JNZ05_001483"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY8564761.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFDYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTDTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="JNZ05_001484"
     CDS             complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="JNZ05_001484"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY8564762.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGHYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="JNZ05_001485"
     CDS             complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="JNZ05_001485"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HAY8564763.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="JNZ05_001486"
     CDS             complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="JNZ05_001486"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAY8564764.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="JNZ05_001487"
     CDS             complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="JNZ05_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HAY8564765.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="JNZ05_001488"
     CDS             complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="JNZ05_001488"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HAY8564766.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14927..16186)
                     /gene="rho"
                     /locus_tag="JNZ05_001489"
     CDS             complement(14927..16186)
                     /gene="rho"
                     /locus_tag="JNZ05_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAY8564767.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="JNZ05_001490"
     CDS             complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="JNZ05_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide rhoL"
                     /protein_id="HAY8564768.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="JNZ05_001491"
     CDS             complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="JNZ05_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HAY8564769.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16973..18238
                     /gene="rhlB"
                     /locus_tag="JNZ05_001492"
     CDS             16973..18238
                     /gene="rhlB"
                     /locus_tag="JNZ05_001492"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HAY8564770.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVETLEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18372..19856
                     /gene="gppA"
                     /locus_tag="JNZ05_001493"
     CDS             18372..19856
                     /gene="gppA"
                     /locus_tag="JNZ05_001493"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HAY8564771.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(19903..21924)
                     /gene="rep"
                     /locus_tag="JNZ05_001494"
     CDS             complement(19903..21924)
                     /gene="rep"
                     /locus_tag="JNZ05_001494"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HAY8564772.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22011..22292
                     /gene="ppiC"
                     /locus_tag="JNZ05_001495"
     CDS             22011..22292
                     /gene="ppiC"
                     /locus_tag="JNZ05_001495"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HAY8564773.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(22338..23813)
                     /gene="ilvC"
                     /locus_tag="JNZ05_001496"
     CDS             complement(22338..23813)
                     /gene="ilvC"
                     /locus_tag="JNZ05_001496"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HAY8564774.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMVDWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            23963..24853
                     /gene="ilvY"
                     /locus_tag="JNZ05_001497"
     CDS             23963..24853
                     /gene="ilvY"
                     /locus_tag="JNZ05_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HAY8564775.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(24850..26394)
                     /gene="ilvA"
                     /locus_tag="JNZ05_001498"
     CDS             complement(24850..26394)
                     /gene="ilvA"
                     /locus_tag="JNZ05_001498"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HAY8564776.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDSHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(26397..28247)
                     /gene="ilvD"
                     /locus_tag="JNZ05_001499"
     CDS             complement(26397..28247)
                     /gene="ilvD"
                     /locus_tag="JNZ05_001499"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HAY8564777.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(28312..29241)
                     /gene="ilvE"
                     /locus_tag="JNZ05_001500"
     CDS             complement(28312..29241)
                     /gene="ilvE"
                     /locus_tag="JNZ05_001500"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HAY8564778.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(29261..29524)
                     /gene="ilvM"
                     /locus_tag="JNZ05_001501"
     CDS             complement(29261..29524)
                     /gene="ilvM"
                     /locus_tag="JNZ05_001501"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HAY8564779.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(29521..31197)
                     /locus_tag="JNZ05_001502"
     CDS             complement(29521..31197)
                     /locus_tag="JNZ05_001502"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HAY8564780.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAIAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGIPKDIQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVG
                     MAQAVPALREFLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLL
                     IAVGARFDDRVTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQP
                     LNINDWQQHCAQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWA
                     AQHIAHTRPENFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGT
                     VKRKQLPLKIVLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHI
                     TRKDQVEAALDTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31200..31250)
                     /locus_tag="JNZ05_001503"
     CDS             complement(31200..31250)
                     /locus_tag="JNZ05_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564781.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(31336..31434)
                     /gene="ilvL"
                     /locus_tag="JNZ05_001504"
     CDS             complement(31336..31434)
                     /gene="ilvL"
                     /locus_tag="JNZ05_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HAY8564782.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            31786..33306
                     /locus_tag="JNZ05_001505"
     CDS             31786..33306
                     /locus_tag="JNZ05_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HAY8564783.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(33331..33669)
                     /gene="maoP"
                     /locus_tag="JNZ05_001506"
     CDS             complement(33331..33669)
                     /gene="maoP"
                     /locus_tag="JNZ05_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HAY8564784.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            33788..34627
                     /gene="hdfR"
                     /locus_tag="JNZ05_001507"
     CDS             33788..34627
                     /gene="hdfR"
                     /locus_tag="JNZ05_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HAY8564785.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLSGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(34723..34798)
                     /locus_tag="JNZ05_001508"
     tRNA            complement(34723..34798)
                     /locus_tag="JNZ05_001508"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34763..34765),aa:Trp,seq:cca)
BASE COUNT         8486 a         9521 c         8788 g         8009 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ccgggactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgct
      301 cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg ctttaccgtg
      361 gcgattaagc ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccgac
      421 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
      481 tgccattgtt atgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
      541 cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac
      601 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
      661 gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac
      721 cggtacgccg tgacgggaaa cttttgccat tgccgccggt aactgacggt ttttcgccag
      781 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt
      841 cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag
      901 tacgaacggg ctgccgttgc tgccaatttc attccacggg aagatggtga cgataacgaa
      961 aatcgcgcct acgtagaaaa tcaggatccg ccacagcact ttgcctacgg cactgcgcag
     1021 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     1081 ggacgccacc acaatacaca gagcggtcag gaaccctttc cagccacccg caaagaaacc
     1141 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     1201 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     1261 ccagaactcg atttcgccgt acaaccgcac cgccgccaga ttcgccaacg ccaccagcgc
     1321 caccgcgatc aatgcgggta tccactgcgc catctccggg gaccaaaact ggacataaac
     1381 gccaatggcg gtgatttctg agatccccac cgccatccac ataaaccagt aagaccaggc
     1441 ggtgagatag ccaaaaaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     1501 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaaa cgaacagccc
     1561 ggcgatgata taggccaaca atacggatgg cccggcccat ttcagggtac tggcggcccc
     1621 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     1681 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     1741 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     1801 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     1861 acaggcgacg aagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     1921 tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     1981 aaagcaggcg gtagagccac tccagtccca gcgtttgcca gattttcggt gcgcgtttta
     2041 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     2101 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     2161 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     2221 aaccatcctg actgccaacg atattcacat tccattggtt gcgcagttta gcttcagttt
     2281 gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc
     2341 gcgccatcag ctcttcccag agatcggcac cggcaacgcg ggaaacctgc gcctgcgggt
     2401 actttttacg tactgaacgt acaacgctga tgccatccgc atatttaaat tcggcagcgt
     2461 taattaactc cctgacctcg gcgttatctt caatggtcag cattttttcg gcattaatgg
     2521 caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg gcatgctgca
     2581 tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt
     2641 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag
     2701 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     2761 cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat
     2821 catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagt
     2881 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagtc caaccacaat
     2941 cgccccgagt gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     3001 cgagatcgcc agtccggagt ggttattcag tacttcccag gtaaagtaat tggctgagtt
     3061 cagcaccata ctcgggcgcc ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     3121 gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     3181 attctcccac ggtgaaaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc
     3241 gccgctcaca ttcattccat agcgttttaa cgccagccag aacatgccaa caatccccag
     3301 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     3361 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag
     3421 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctgt cctggcgcag
     3481 aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     3541 ggagacttca ctggaaaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     3601 gtgcatgaag aagatgccga cgcttaccag cgcgataccc atcaggatca cccacgtaag
     3661 attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcag caacgcgttt
     3721 gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg cagaaagcaa
     3781 cgcctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     3841 ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     3901 gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag
     3961 cagccagacc acaaacaggc cactgaattg cagcagactc atgcgacctc cccggcggca
     4021 atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt
     4081 ttatccgccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg
     4141 gtagtaaaca gcactggcaa atgctgttcc gtcatatcct gccagaacgg attttccctg
     4201 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaaa gcgtaccaat gccctgctgg
     4261 cgggcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata ggcgtcaaat
     4321 tccagttttt cgctcaaaat ttgcagattt tcttcgctga ataactccag ccccgcctga
     4381 cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt
     4441 accgtatcgc caaattgctg atgaacggca cgtaaagcgg cgacatgctc attgctgcgg
     4501 tcgccggagt tccccaccag aatggtcatt tttccttcac gttgccgatc gttcgccatc
     4561 gtattgagag aagggtccat ccgcgtcggg aagtacagca gttctccccg cacctttggg
     4621 tgcgttttgg caaaaaagct caaatcgccg cgggtggcaa atacacagcc gactcgcttt
     4681 tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagctcatac
     4741 aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc actcagcaga
     4801 gccagccaca gtgtgggatt gaactgaccg tggaaaaaaa atcgctgctg acggttagct
     4861 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     4921 agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcacgcg
     4981 tgctcgctcg tcgcggccag cgcgtcattg aaaaaccgca aaacggttcg gttatggtga
     5041 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     5101 acacgccaca acaaagagaa aaatagacga tataagttgc catatatgcc tgcgccgcgc
     5161 ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     5221 tgacttccgc cagaatataa aaccgcagtg acgctttggc gatcaccaga taaccaaaga
     5281 cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga tcgcgcataa
     5341 cggtaaattt attcgacaac agcaaccaga tagcaaaatc acgcagcaac cagacggtaa
     5401 aactcgctgc cgccactgcc ggtaagacga atttcagcga tttaaccact tcccgggtga
     5461 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     5521 taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacttcat
     5581 cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     5641 ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc
     5701 agctgggttt cagatagctt aacgggatga caccacgttt aatcaacatg atggcggcag
     5761 gaattaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccca
     5821 aacggtaact gacgtagtac gcgagaacgc caatcaagct gccgacaatc agcgataacg
     5881 cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg ccccaggcga
     5941 tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta ttaccaaaca
     6001 atccctggct gattggcgca gctgccagca caaaaaccag cgccatcaac gtagagaaac
     6061 caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt
     6121 actgggcaac gtatttggtt acaccgttaa agatgccagc cccggcaagc acgccgagca
     6181 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacaccg
     6241 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccgccgtcc
     6301 acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc
     6361 gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcgctc
     6421 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agtgttcccc cgcagggcaa
     6481 ccgtgcagcg gaatgtaatg aaacaccgcc atgatttccg cttctttcag aaagttaatc
     6541 aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg
     6601 cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg cgctaacgta
     6661 tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc tgcttccagt
     6721 tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc aatatcgcgc
     6781 caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc cttttcacgg
     6841 atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc gccttcacca
     6901 cccgccgtgt agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc
     6961 agtgcacgcc ctttgtaagt ggacatcacg ccctgagcgg catcttccac cacaaacaaa
     7021 ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg
     7081 accggcacga taacgcgcgt tttgtcggtg atctctgctt caatcaacgt ttcgtcgatg
     7141 ttcatggtgt ccgggcgaac atccacaaag acgattttcg cgccacgcag cacaaaggca
     7201 ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
     7261 agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg
     7321 ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
     7381 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
     7441 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
     7501 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg ccacccgcaa
     7561 agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctgc
     7621 accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc gtaaagagac
     7681 atagccgcga atatcgccgg acgccgcacg tagaatcaga cattgatgat caaaggtgcc
     7741 gcgcacggca ttttcaatcc actgtgcata aaagcgaccg ctggcgtcag gcgcatacca
     7801 cggcgcacga aaacggcttt gcgcaaatgc ggcgctagct aactgacgta atgcgggaat
     7861 atcggtctct tgtgccacga cagcaccgct atcactgaca ttgttcacgg gtagcgccaa
     7921 atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca gttcccccgt
     7981 atttgatgcc gcaattttgg cctgcacccg tgaccatggc gctaaggcgt ctgacgtcag
     8041 gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga aggcgttttc
     8101 ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc
     8161 cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca tcgagccagc
     8221 cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt ttttctactg
     8281 tctgcacaaa agtgctggcc tcaatcaggc tgtcgtgagt gtcggtatcc agccaggcaa
     8341 atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc tggttgatgg
     8401 aggtaatctc cagttcaccg cgctccgacg gcttcacctg ctttgcgtac tccacgactt
     8461 tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt tttggctttt
     8521 cttcaagcga gatagcgcgg aaattgtcgt caaattccac cacgccaaag cgttccgggt
     8581 ccatcacctg atagtcaaaa accgtcgccc cttccgtgcg cgccgcaaca tgacgcagct
     8641 tcggactgaa gccctgaccg aagaagatgt tatcgcccag caccagacaa gaaggttcac
     8701 cattaaggaa ggtttcaccg atgataaagg cctgcgccag accgtccggg ctgggctgtt
     8761 cggcatattc cagctggata ccgaactcac taccatcgcc cagcaggcgc tggaaataac
     8821 ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccagc atcagcaccg
     8881 acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc gatacgccgc
     8941 gcgtaatcgg atgcaatcgg gtaccggaac cgcccgccag gataataccc ttcatgtttg
     9001 cctccgctga aaattagcct ttcagaccta aacgctcacc ctgatagctg ccgtcctgca
     9061 cctgcttcca ccagctttca ttagccagat accactgaac cgttttacgc attccacttt
     9121 caaaggtttc ctgcggcagc cagccaagtt cacgggcaat tttcgaagca tcaatggcat
     9181 aacgcagatc atgccccgga cggtcagcga caaaggtgat caggtcacga tagtgcgcca
     9241 cgccgtgcgg cttgttcgaa gccagttctt ccagcagctc gcaaatggtt tccacaacat
     9301 cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact ttcccggtgg
     9361 tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca cggatttgct
     9421 gcccgttgcc atataccggc agcggtttac ccgccagcgc gttgaggatc atcagcggga
     9481 tcagtttttc cggaaagtgg taatgaccgt agttattcga gcagttggtg atcagcgtcg
     9541 gcagaccgta ggtccgcagc caggcgcgca ccagatggtc gctgctggct tttgacgcgg
     9601 aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca
     9661 ggtcaccata tacttcgtcg gtggagatat gatgaaaacg gaacgctgat tttttatctt
     9721 ccgtcagcgt attccagtaa gcccgcgccg cttcaagcaa tgtataagtc ccgacaatgt
     9781 tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt tcggctgcca
     9841 gatgcatgac acagtctggc tgatgctcag tgaatacgcg tgccagttct gcccgatcgc
     9901 agatatcaac tttctcaaag gcaaagcgct cgctttgcgc gaccggtgcc agcgacatca
     9961 ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc atcgctcgtt tcgttgatga
    10021 tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga atttttctca
    10081 tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt cgccattgat
    10141 aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg ccagcgcctc
    10201 gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt ggatgttagg
    10261 ctcaacaacc agcgtttcgc cgctgtgcca ctgggcgatc agttcagcga tttccatcgc
    10321 cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc aggcgatttt
    10381 cagttcactg gcgcgtttat cggtagcagc aaggcagtcg gcaaccgccg ctttcacctg
    10441 atcgataacc cagaacggtt tgtgatcgtt aacttcgcgc gcggtacgga taagccgcgc
    10501 ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt gaccgcccac
    10561 gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc gaatcagttc
    10621 ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa aagcgatatt
    10681 cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg agttagtgac
    10741 gacacactca ccttcgagga aaattttgta caattcgctg gcgcgggccg aacaaaccgg
    10801 cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct ggcccggtaa
    10861 tacacgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct gctgtgggaa
    10921 agtcagatcc gggcgcatct ctgctaacca ttcagccatc ttctcggttg accccaccgg
    10981 cgaggtggat tcaaggatca ccagggcgcc ttttttcagc actggcgcaa tggagcgagc
    11041 agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg taggtacagc
    11101 aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac cgccttctac
    11161 ggcagttttt actacgctcg ccaaatcagg ctcgacgata tggatttcgc cacgattgat
    11221 ggtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc gtgaggcaaa
    11281 cgctgctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg tcgcaaaact
    11341 catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc ctgaccatca
    11401 ccatacgggt tatgggcgcg gctcatagct tgatattcgt tttcgtcttt taaaagacgc
    11461 gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt acccgccgtc
    11521 accgcttccg gacgctcagt ggtatcgcgc atcaccagca ccggtttccc cagcgaaggc
    11581 gcttcttcct gaatgccgcc tgagtcagtc aaaatcagcc aggcgtggtt catcagccag
    11641 acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacatg ccccagaatg
    11701 cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat ctggatgtcc
    11761 tggtgcgtgg tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc acgaccgaaa
    11821 ctctcacgcc tatgaccggt caccagaatc atctttttat cgggggcgat aaacgggtaa
    11881 tttgccgcca gttctgaacg cagcgtgtcg ctgctcatca cctggtcacg cacccataac
    11941 agtgcatcaa tgactgtatt accggtaatg aagattcggc tatccgcaac gttttcacgc
    12001 agcaagtttt gccgggaagt ttcggttgga gagaagtgat acatcgccag atgcccggtc
    12061 aatgtacggt tagcctcttc cggccacggc gaatagagat cgcccgtgcg cagaccagcc
    12121 tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc cagcgtcgtc
    12181 gtcgtatcgc cgtgaaccag cacaacgtct ggtttgaact cggcaagaat aggttttagc
    12241 ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat tatgttgaga
    12301 tcgtagtcag gtacaatgga aaagagtttc agtacctgat caagcatctc ccgatgctgc
    12361 gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg caccaacgga
    12421 gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac atcgattctc
    12481 ttcgaataag cggcgagcgc ctttgcgctc accgcagcag tgttgctatt tcgagcaacg
    12541 gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca taatcatcag
    12601 gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca aatagcgata
    12661 ggtctgaaaa cgcggatcca gggttggacc aacattcagg gtgtttaaca tggctcgatt
    12721 ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca gtcgagcctg
    12781 gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg caggcacatc
    12841 tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct caatgctgtt
    12901 catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct gagctttcat
    12961 ctggatggtg cgtgccgccc atgcgccttt cagctcatca ttcagatggc tggctgcacg
    13021 ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg cggtttcggc
    13081 aataagcttc acgctgtcat tgaccgcgcg agtaaagtct ccggggataa actggatgtt
    13141 gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca tccgctgttt
    13201 gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca gttgcataac
    13261 aaactcttta taggcttcgt ccatgaccga tggttgatcg gcagaagcca tgtttgaacg
    13321 gacatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca tattcaccgt
    13381 tggacgatcg gtaatcgccg tcgagctcca ctcctgacga gcaaaaaaag tatacgccag
    13441 cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag cccacaaggt
    13501 acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt tcccaggcat
    13561 tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc acgatttcta
    13621 cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg cttaatgcaa
    13681 tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc cgggacaaaa
    13741 tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag ggtaatcagc
    13801 acaaacgcct gacgggaagt aaacccggca cgcatgatca aatggtgaat atgctgacgg
    13861 tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc caccatatcc
    13921 attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg ggttttgccc
    13981 tgggtcgttt cgagcaggat ccagataacg gtaaaaccaa tcagcgtact gcccgcatca
    14041 cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat gtatggcagg
    14101 atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc gaaccacaaa
    14161 atcataccga ttgctgcaaa cgagacgcag gacaacccgc ccagcaagcc atcaatgcca
    14221 tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag gaagtaacca
    14281 aacggtccga gcaccatctc ccaggagcca aagatataac ccaggctact gagataaagt
    14341 ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg gatttttacg
    14401 ctgatatcaa aacgatcatc cagcgcgcca atgaaaacaa gcacaccggc acaagcgaga
    14461 tagagagatg catgcggaat atagtaatcg acaattccga acgtgaagca aatccctgcg
    14521 taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg gaagtttggt
    14581 ttatccacta aaccgacttt ttttgccacc ttacgggcaa aaaacagaaa cagtgtcgtg
    14641 aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat gcatgctctc
    14701 agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg gaaagtccgt
    14761 tccgaaccca ccagccctgt gcaaaaatta accatttgat atgcaaattg cataacatgc
    14821 cagcaaattc caggaatttt cctaacatcg ccagcgcgac attaagatta cagatataaa
    14881 agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag accaatttat gagcgtttca
    14941 tcatttcgaa gaaatcgtca ttggtcttgg tcatggccag tttattaatg aggaattcca
    15001 ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt ttctgcagtt
    15061 cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag tcgatagccg
    15121 ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg cctgtacctt
    15181 taaactcttc gtaaataact tcgtccattt tagaaccggt atcgataagc gccgtcgcga
    15241 taatggtcag gctgccgccc tcttccacgt tacgcgccgc accaaagaag cgtttcggac
    15301 gatgaagggc attggcgtcc acaccaccgg tcaacacttt acctgacgcc ggaacaacag
    15361 tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag aatgataacg tctttcttgt
    15421 gctcaaccag gcgtttggcc ttctcgatca ccatttccgc aacctgaacg tggcgagatg
    15481 cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc atctcggtta
    15541 cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg tggttgtaag
    15601 caatgctctg agcaatgttc tgcagcagca tagttttacc ggctttcggc ggtgccacaa
    15661 tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga gcagttaaat
    15721 cttcagtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc agcggggtta
    15781 agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa
    15841 ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta ccagagatgg
    15901 tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag atgtcatcag
    15961 gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc tgcaatatct
    16021 ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc aggatggcaa
    16081 aaataatgtc ctgcttacgc atgcgagcca ggttttccag ccccatattt tcgccgagag
    16141 tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg gtgtgagttc
    16201 ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga tcatccatgc
    16261 ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag tacgcagaac
    16321 tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc atttaagata
    16381 aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt aatacgaagt
    16441 caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa
    16501 catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac ctttagacag
    16561 tgcgcccact ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc
    16621 acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt
    16681 cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg
    16741 accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt
    16801 gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact ccacaggaat
    16861 aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg ttgactttat
    16921 ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca ctatgagcaa
    16981 aacacattta acagaacaga agttttccga cttcgccctg catccgaagg ttgtagaaac
    17041 ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc ttccgctgac
    17101 gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt
    17161 tcttacgtca acgtttcatt atcttctctc tcatcctgcg attgccgatc gcaaggtgaa
    17221 tcaaccgcgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga tccacgccga
    17281 cgcggaaccg ctggcggaag ctaccggcct gaagctgggc ctggcttacg gtggtgatgg
    17341 ctatgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg gcaccacggg
    17401 tcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc aggtagtggt
    17461 actggacgaa gccgatcgca tgtacgatct aggctttatt aaagatatcc gctggctgtt
    17521 ccgccgtatg ccgcctgcaa atcagcgcct caacatgctg ttctccgcca cgctttcgta
    17581 ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg aagtggaacc
    17641 ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta acgaagaaaa
    17701 aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga ttattttcgc
    17761 caataccaaa caccgttgtg aagagatctg gggccacctg gcggcagatg gtcatcgtgt
    17821 cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac
    17881 ccgtggcgat ctggatattc tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc
    17941 ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt acgttcaccg
    18001 tattggtcgt acaggtcgcg caggcgcaag cggtcactct atcagcctgg cgtgtgaaga
    18061 gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc cggtaagcaa
    18121 atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca cgcgcccgcg
    18181 cacaggcaat ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaat
    18241 aaaatgtcat actcaaaatc attcaaactg tatcaagatg gcaagagaat gaatcccgat
    18301 gagcttactg tagtaagtga ttcgggtgaa agaatgcagc caacacagag acagattgaa
    18361 ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc ggttcgaata
    18421 gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg acgcgaatta
    18481 aacgcaaagt gcgtctggct gctggcctga atagcgaaaa tgacctctct aatgaagcaa
    18541 tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat atccctccct
    18601 cgcaaattcg cgttgtcgct acggcgacat tacgacttgc cgtcaatgcg ggtgatttta
    18661 ttgccaaagc gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt gaagaggaag
    18721 cgcgtctgat ttatcagggc gttgctcaca ccactggtgg tgccgatcag cgcctggtgg
    18781 tggatatagg cggtgccagt actgaactgg taaccggcac gggtgcacaa accacctcgt
    18841 tgttcagcct gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc gatcgtaatc
    18901 tggggcaaga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg
    18961 ccgatgaatt acggtatcac ggctggaaag tgtgcgttgg tgcttccggc accgtgcagg
    19021 cgttacagga aatcatgatg gcacagggga tggatgaacg cattaccctg gaaaagttgc
    19081 agcaattgaa acagcgagcc attcattgcg gtcggctgga ggaactggag attgacgggc
    19141 tgacgctgga acgtgcgtta gtgttcccga gtggtctggc gatcctgatc gccattttta
    19201 ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct
    19261 acggcatgtt gcatctggcc gtcgagcagg atattcgcag ccgtacactg cgtaatattc
    19321 aacgccgctt tatgatcgat attgatcagg cacagcgcgt agccaaagtt gcggctaact
    19381 tcttcgatca ggtggaaaac gaatggcatc ttgaagcaat aagccgcgat ttgctcatca
    19441 gcgcctgtca gcttcatgaa atcggcctga gcgttgactt caaacaagct ccgcaacacg
    19501 ctgcttatct ggtgcgtaac ctggatcttc ccggttttac ccccgcacag aagaaactgc
    19561 tggcgacgct actgctcaac cagactaacc cggtcgatct ctcatcgctg catcagcaaa
    19621 atgccgtacc accgcgcgtc gcagaacaac tctgccgttt actccgcctg gctatcattt
    19681 ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct aaccatgaac
    19741 tgttgacctt gacgcttccg caaggttggc taacccaaca tccgctgggt aaagagatta
    19801 ttgctcagga aaaccagtgg cagagctatg tccactggcc gctggaagtg cattaatgag
    19861 taagtgccgg atgcgatgct gacgcatctt ctccggcctt gattatttcc ctcgttttgc
    19921 cgccatcatc gctttcagat tcgccagatg gctttgccct ttctgcatcc gttcttcggc
    19981 actgaccact ttgcgctcct gttcccaaat cagatcatcc tgcggcagct ccagcaaaaa
    20041 gcggctcggc tccgggcgca ccagttcgcc gtactgacgg cgttctttac acagcgtaaa
    20101 ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct cctcatcgat
    20161 attatcttca tcgatactgc tctggtgcgg caaaaaccct tcttccatac cgaccatgta
    20221 gacataagga aactccagcc cttttgacgc atggagagtc atcagttgca cctgatccag
    20281 ctcttcttca ctctcaccac gctccatcat gtcgcgtaaa gtaaagcgcg tcaccacctg
    20341 ggtgagcgtc atcggctcat ccagttcgct gccttccagc atctccgtca tccagctaaa
    20401 cagttggttg acgttcttca tgcgcatttc ggcagctttc gggctgggcg atgtttcgta
    20461 cagccaggat tcataatcca tgccgtggat cagatcgcgc accgcggcaa tcggctcccg
    20521 ctccgccaga cgctggattt ctgccaacca gtgagtgaag cgggtcaatg cttcataacc
    20581 acgtccgcta agcgtctgac tcaggcccat atcaaagctg gcggtaaaca tgcttttatt
    20641 gcgcgtcatc gcccactcac ccagcttttt cagcgtcgcc gggccaatct ctcgcttcgg
    20701 cgtgttaacg atacgcagaa atgcgctgtc atcgtccggg ttagtcagca cgcgcagata
    20761 agccagcaag tccttgattt caggacgaga gaaaaacgac gtaccaccag aaattttgta
    20821 cgggatgcgg ttttgcatca ggaatttttc aaacacccgc gactgatggt taccgcgata
    20881 aagaatagcg taatctttgt actgcgtttt attgacgaag tgatgcgcaa tcagttcacc
    20941 agtgacgcgc tcggcttcat gttcttcgtt attcgcgctt aaaactttta gctctgtgcc
    21001 gtaacccagt tcggagaaca gacgcttttc aaagacgtgc gggttattgg cgatcaggat
    21061 gttcgccgct ttcaaaatac gtccggaaga gcggtagttc tgctcaagct taatcacctt
    21121 cagcgccgga aaatcctggc tcagcagcac caggttttgc ggacgtgcgc cgcgccagga
    21181 gtagatcgac tggtcatcgt cacccaccac ggtaaagcgc gcgcggctgc ccaccagcag
    21241 tttcaccagt tcatactggc tggtgttggt gtcctgatat tcgtccacca gcaggtagcg
    21301 aattttgttc tgccagcgct cgcggacttc ttcattgcgt tgcagaagta gcgtcggcaa
    21361 taaaatcaga tcatcgaagt cgagcacatt acaggctttc aggtgtgcat catacagccc
    21421 ataacaatgg gcaaaaatac ggtcccgctc gccaatcgca ctggctgccg cctgggacgg
    21481 tgttttgaga tcgttcttcc agttagagat ggtcgaaatc aactgttgca ggagaacttt
    21541 gtcatcttca atcagccctt cggtcaactc tttgagcaac gccagctgat cggtatcgtc
    21601 aaacagcgaa aagttcgcct tcatccccag cgccgcatac tcacgcttga tgatatccag
    21661 ccccagcgtg tggaaggttg agatcatcag cccacgcgcc tctttgcgac ccagcgtctg
    21721 tccgacacgc tctttcatct cgcgcgccgc cttattggta aaggtcaccg ccgcgatatg
    21781 tcgcgcctga taaccgcacc cgcggatcag atgggcgatt ttattggtga taacacgagt
    21841 tttaccggaa cccgcacccg ccagcaccag gcaggggccg gtaacgaatt cgacagcttg
    21901 ttgttggccg gggtttagac gcataggaaa atcgctcaat gaaagtcggg aggggaaaat
    21961 aacagcgtgg tagtatagcc agcctcatcc ttaccactca aggcacgatc atggcaaaaa
    22021 cagcagcagc actgcatatc cttgtaaaag aagagaaact ggctctggat cttctcgtgc
    22081 agattaagaa cggtgccgat ttcggcaagc tggcgaagaa acactccatt tgcccatcag
    22141 gcaaacgcgg cggtgattta ggtgaattcc gccagggtca gatggttccg gcgttcgata
    22201 aagttgtgtt ctcttgtccg gtactggagc caaccggccc gctgcacacc cagttcggtt
    22261 accacatcat taaggtgctg taccgtaact aatagcaagg ccttctccgg gagaaggcct
    22321 tgaactctga atcgctatta acccgcaaca gcaatacgtt tcatatccgt catatagccg
    22381 cgcagtttct tacctacttg ctcaatcgca tggctgcgaa tcgcttcatt tacatcacgc
    22441 agctgcgcgt tatctaccgc accttccgga atagctttac ccaaatcgcc cggttgcagt
    22501 tctgccataa acggtttcag cagcggcaca caagcgtaag agaacagata gttaccgtac
    22561 tcggcagtat cagagataac cacgttcatt tcatacagac gcttacgggc gatggtgttg
    22621 gcaatcagcg gcagttcgtg cagtgattca tagtaagcag attcttcgat gatgccggaa
    22681 tcaaccatgg tttcgaacgc cagttcaacg cccgctttca ccatcgcgat catcagtacg
    22741 cctttatcga agtactcctg ctcgccgatt ttgccttcat actgcggcgc ggtttcgaag
    22801 gcggttttgc cggtctcttc acgccaggtc agcagtttct tatcatcgtt ggcccagtcc
    22861 accatcatac cggaggagaa ttctccggag atgatgtcgt ccatatgttt ctggaacagc
    22921 ggtgccatga tctctttcag ctgttcagac aacgcgtaag cacgcagttt cgccgggtta
    22981 gagagacggt ccatcatcag tgtgataccg ccctgcttca gcgcttcggt gatggtttcc
    23041 caaccgaact gaattagttt ttctgcgtat gccggatcgg tgccctcttc caccagcttg
    23101 tcgaagcaca gcagcgagcc agcctgcaac ataccgcaca ggatggtttg ctcacccatc
    23161 aggtcagatt tcacttccgc aacgaaggac gattccagca cgcccgcacg gtgaccaccg
    23221 gttgctgccg cccatgcttt agcgatcgcc atgccttcgc ctttcggatc gttttccggg
    23281 tgaacggcaa tcagcgtcgg tacgccgaat ccacgtttgt actcttcacg cacttcggtg
    23341 ccagggcatt tcggcgcaac catgactacg gtgatgtctt tacggatctg ctcgcccact
    23401 tcgacgatgt tgaaaccgtg cgagtaaccc agcgccgcgc cgtctttcat cagtggctgt
    23461 acggtgcgca ctacatcaga gtgctgcttg tccggcgtca ggttaaccac cagatccgcc
    23521 tgtgggatca gttcttcgta agtacccact ttaaaaccat tttcggtcgc tttacgccag
    23581 gacgcgcgct tctcggcaat cgcttcttta cgcagagcgt aggagatatc gagaccagaa
    23641 tcacgcatgt tcagaccctg gttcagacct tgtgcgccac agccgacgat gactactttt
    23701 ttaccctgaa ggtagctcgc gccatcggcg aattcatcgc gccccataaa gcgacatttg
    23761 cccagctgtg ccagctgctg acgcagattc agtgtattga agtagttagc catggtgatt
    23821 cctcgtgatg ttgtgcttct tattgttcgg ttcgcgtttg cgagattgaa ttcactatat
    23881 gacaggaaat ttattgcgga aattgatata ttcacaacgt cacattgcaa tttctgcaac
    23941 gtcaacattg agggctgcgc cattggattt acgcgatctg aaaaccttcc tgcatctggc
    24001 ggaaagccgc cattttggcc gcagcgcgcg ggcgatgcac gttagcccgt ccacgctctc
    24061 acggcagatt cagcgcctgg aagaagatct cggccagccg ctgtttgtgc gcgataaccg
    24121 cacggtgacg ctgactgaag cgggcgaaga actgcgcgtt ttcgcccagc aaacgctgtt
    24181 gcagtatcag cagctgcgcc acaccatcga ccagcaaggg ccgttgctct atggcgaatt
    24241 acatatcttc tgctcggtaa ccgccgctta cagccatctg ccgccgattc tggatcgctt
    24301 ccgcgcggaa cacccgtcgg tggagatcaa actcactacc ggtgatgcgg cagatgcaat
    24361 ggaaaaagtc gtcacaggtg aagctgatct ggcgattgcc gggaagccag agactctgcc
    24421 cggcgcggtg gcgttttcga tgctggagaa tctggcagtg gtgttgattg cacccgcatt
    24481 gccctgcccg gtgcgtaatc aggtttctgt cgaaaagccg gactggtcaa cggtaccgtt
    24541 tatcatggcc gatcaggggc cggtgcgccg ccgcattgag ttgtggttcc gacgcaataa
    24601 aatcagtaac ccgatgattt acgccacggt tggcgggcat gaagcgatgg tatcgatggt
    24661 ggcgctcggc tgtggcgtgg cattgttgcc ggaagtggtg ctggaaaaca gccccgaacc
    24721 ggtgcgtaac cgcgtgatga ttttagagcg cagcgatgag aaaacgccgt ttgagctggg
    24781 cgtgtgtgcg cagaaaaagc ggctacatga gccgctaatt gaggcattct ggaagatttt
    24841 gccgaaccat taacccgcca aaaagaacct gaacgccggg ttattggttt cgtcgtggca
    24901 atcgtagccc agctcattca gccgggtttc gaaatccggt tcatggtcgc caagttcgaa
    24961 cgccgccagt acgcgcccgt agtcggtgcc atggctgcga tagtggaaca aagaaatgtt
    25021 ccagtgcgta cccagcgtgt tgaggaagcg cagcagcgcg cccggtgatt ccgggaattc
    25081 gaagctgtag aggcgttcct gcaacggatg tgacggacgc ccgccgacca tatagcgcac
    25141 atgcaacttc gccatttcgt cgtcggagag atccaccacg ctgtagccgc cgtcgttgag
    25201 catctgcaaa atttctttgc gctcttcgag gccgcggctc aggcgcacgc cgacaaagat
    25261 gcaggcgttt ttggcatcgg caaaacggta gttgaactcg gtgaccgatc gcccgccaag
    25321 cagttggcag aatttgagga agctgccttt ttcttccgga atggtcaccg ccaacaacgc
    25381 ttcacgctgt tcgcccagtt cgcagcgttc cgagacgtag cgcaggccgt ggaagttcac
    25441 gttggcaccg gaaagaatat gcgccagccg ttcgccgcga atgttgtgct gggcgatata
    25501 ttttttcatt cccgccagcg ccagcgcgcc agagggttcc gccaccgcac gcacatcttc
    25561 gaataaatcc ttcatcgccg cacagatcgc atcgctatcg acggtgatga tgtcgtcgag
    25621 atactcctgg cataaacgga aggtttcgtc accgatgcgt tttaccgcga caccttcggc
    25681 gaacagtccc acgcgtggca gatcaaccgg atgacccgca tccagcgctg ctttcaggca
    25741 ggcggagtct tccgcttcta cggcgatcac tttgatttgc ggcatcagtt gtttgatcag
    25801 caccgctacg cccgccgcca gaccgccgcc gccgactggc acaaatacgc ggtcgagatg
    25861 ggagtcctgc tggagcagtt ccagcgccag cgtgccttgc ccggcaatca ccatcggatg
    25921 gtcgaacggc ggcacccagg tgaacccctg ctgctgtgac agttcgatcg ctttagcttt
    25981 cgcttcgtca aagttcgcgc catgaagcag cacttcgccg ccgaatccac gtaccgcatc
    26041 gactttgatg tcggcggtgg cggttggcat aacgatcagg gccttcacgc ctaaccgcgc
    26101 agaagaaaac gcgacgccct gcgcgtggtt acccgcagaa gcagtgatca cgccgtgcgc
    26161 tttttgttct tccgtcaggc ccgccatcat ggcgtatgcg ccgcgcagct taaagctgtg
    26221 cactggctgg cgatcttcgc gcttcaccag aatgacgtta tcaagacgcg acgacagttt
    26281 ttccattttt tgtagcggcg taacctgcgc cgcctcgtaa accggcgcgc gcagcactgc
    26341 tcttaaatat tcggcacctt ccggagcacc ggacaggggt tgcgagtcag ccattattaa
    26401 ccccccagtt tcgatttatc gcgcaccgca cctttgtcgg cgctggttgc caggctggcg
    26461 taggcgcgca gggcaaagga aacctgacgt tcacggtttt tcggcgtcca ggctttgtca
    26521 ccccgagctt cctgcgcttc acgacgcgcc gccagttcgg catcgcttac ctgtaactga
    26581 atgccacggt tcgggatgtc gatagcgatc aggtcaccat cttcaatcag accaatgctg
    26641 ccgccgcttg ccgcttccgg tgagacgtgg ccgatggaaa gaccagaggt gccgccagag
    26701 aaacgaccgt cggtgatcag cgcacaggct ttaccgagac ccattgattt caggaagctg
    26761 gttgggtaga gcatttcctg catgccaggg ccgcctttcg ggccttcata gcggatcacc
    26821 accacatcgc ccgcgacaac tttgccaccg aggatcgctt ctaccgcgtc gtcctggctt
    26881 tcgtacactt tcgccgggcc ggtgaatttg aggatgctgt catcgacgcc cgcggttttc
    26941 acgatgcagc cgttttcagc aaaattaccg tagagcaccg ccaggccgcc gtctttgctg
    27001 taggcgtgtt ccagcgagcg gatacagccg tttgcgcgat cgtcatcgag agaatcccaa
    27061 cggcaatcct gcgagaatgc ctgtgtagta cgaatgcccg cagggcctgc gcggaacata
    27121 ttttttaccg cgtcatcctg ggtcagcata acgtcgtatt gttccagagt ttgcggcaac
    27181 gtcaggccaa gtacgttttt cacatcacgg ttcagtaacc ccgcgcgatc cagttcaccg
    27241 agaataccga taacaccacc agcacggtga acatcttcca tatggtattt ttgggtgctc
    27301 ggcgcaactt tgcataactg cgggacttta cgcgaaagtt tgtcgatatc actcatggtg
    27361 aagtcgattt ccgcttcctg cgccgccgcc agcaggtgaa gtaccgtgtt ggtagagccg
    27421 cccatcgcga tatccagcgt catggcattt tcaaacgccg ctttgctagc aatattgcgc
    27481 ggcagtgcac tttcgtcgtt ttgctcgtag taacgtttgg tcaattcaac aatgcgttta
    27541 ccggcattga ggaacaactg cttacggtcc gcgtgggttg ccagcagcga gccgttgccc
    27601 ggctgcgaca ggcccagcgc ttcggtcagg cagttcattg agttagcggt aaacatcccg
    27661 gagcaggagc cgcaggtcgg acacgcggaa cgttcaacct gatcgctctg ggagtcagaa
    27721 actttcgggt ctgcgccctg gatcatcgca tcaaccagat cgagtttgat tatctgatcg
    27781 gacagtttgg ttttcccggc ctccatcggg ccgccggaaa caaagatcac cggaatattc
    27841 aggcgcaggg aagccatcag catccccggg gtgattttgt cgcagttaga gatgcagacc
    27901 atggcgtcgg cgcagtgggc gttgaccata tactcaacgg aatcagcgat cagttcgcga
    27961 gatggaagtg aataaagcat ccccccgtgg cccatggcaa tcccatcatc caccgcaatg
    28021 gtgttgaact ctttggcaac gccgccagcc gcttcaattt gttcggcgac cagtttaccg
    28081 agatcgcgca gatggacgtg acccggtaca aattgggtga acgagttcac aaccgcgata
    28141 atcggcttgc caaaatcggc gtcggtcatt ccggtggcgc gccacagcgc acgagcaccc
    28201 gccatattac gaccatgagt ggtggtggcg gaacggtact taggcatact ttatttactc
    28261 ccagtgtctg tctcgtaaat gggacggtgc gtgccgtccc attttttgta tttattgatt
    28321 aacttgatct aaccagcccc atttatcttc ggtttcgcca gtgaagaggc cgaagaaggc
    28381 ttgctgaatg cgtttggtaa ccgggccaca acggccttcg ccaatctgaa taccgtctac
    28441 gctgcgcact ggcgtgattt ctgccgccgt accggacata aagacttcat ccgccaagta
    28501 cagggattcg cgcgacagca cctgctcacg cacttcaatt cccagctctt tcgccagttt
    28561 gatgatggca tcacgggtaa tacccggcag cgcggaggag gtgaacggtg gggtgaacag
    28621 cacaccatct ttcacttcaa acaggttttc gcctgcgcct tcagagatat aaccgttcac
    28681 atccagcgcg ataccttcct gataaccgtg gcggcgcgct tcgctaccca ccagcaggga
    28741 agagagatag ttaccaccgg cttttgccgc agttgggatg gtgtttggtg ctgcgcggtt
    28801 ccaggaggaa accatcgcat cgatcccctg ctccagcgct tctgcgccca gatacgctcc
    28861 ccacgggaaa gcggcgataa tcacatcggt tgagtatccc gctggcgggt ttactcccat
    28921 gccaacatca ccgacgaaga tcagcggacg gatataggcg ctggtgagat tgtttttgcg
    28981 gatcacgtca cgacaagctt ccatcagctc atcaatgctc tgtgaaaccg ggaagcgata
    29041 gattttggcg gagtcatgca gacgctgcat atgctcacga tggcggaata caaccggtcc
    29101 tttgtgtgag tcgtagcaac ggatgccttc aaaaaccgag gtgccatagt gcagcgcgtg
    29161 cgacatcaca tgcaccttcg cgtcttccca gcgaaccatc tccccattga accaaatgta
    29221 atcagctttc ttcgtggtca tttttatatt ccttttgcgc tcaggcgcgg atttgttgtg
    29281 atgtggttgt gctctggcag atggcaacgt gtgcgacgtc caccagttta tttaactgac
    29341 taaacagtaa gtcgaccgac cgtgggctgg caacggtcaa ttcgatattt atattttgtg
    29401 catcgctggc ggcagccata ttcattgagc agacgtggaa accacgatga cgcaccacgc
    29461 gtaaaacacg ttctaaggtt tccggattga agcgagccga tacattgacc tgatgttgca
    29521 tcatgataat ttctccaaca tttctgaatt actggcacct ggcggcacca gcggccagac
    29581 gttctcaagt tcgtcgattg agacatgaag caggtatggc ccatcactgt tcagcatggt
    29641 gtcgagtgcc gcttcaacct ggtctttacg ggtaatgtgt tggccaggga tgccgaaggc
    29701 gctggctaac atgaggaaat cggggttatc ggtaagggtg gtttcgctgt atcgttcctg
    29761 aaaaaacagt tgctgccatt gtcgaaccat ccctaaccgt tggttatcga gtaagacgat
    29821 tttcaacggt aactgcttgc gttttacggt gcccagctct tgcacattca tcatgaaaga
    29881 gccgtcaccg gagatacaga caacggtatc gttcggtcgc gcgacttgtg cgccaactgc
    29941 cgccggtaaa ccaaaaccca tggtacctaa gccgctggag gtgatgaaat tttccgggcg
    30001 agtgtgggcg atgtgctgcg cagcccacat ctggtgctgc cccacatctg tggtcacgac
    30061 gcaatccgca ggtttacgat ccgacagttg ttttaacaac aacggagcgt agatagcgtc
    30121 accgggatgg tcgtaacgcc aggaatgttc atcacgcagc tgcgcgcagt gttgctgcca
    30181 gtcattgata tttaacggct gctgtaatgc tggtaacaga gcatttaaat caccttgtaa
    30241 tgccacatgt gcctgacgca gcttgttcat ttctgccggg tcgatatcca tatggataac
    30301 actggcgtgt ggtgcgaagg tgttcagttt gccggtcacc cggtcatcaa aacgtgcacc
    30361 cacggcgatc agcaggtcac actcctgcac cgcgaagttt gccgctttgg ttccgtgcat
    30421 ccccagcatg cccagatagt acggataatc tgcttctact gcgcccagcc ctttcagcgt
    30481 acaggtggca ggcatttttg tggcagcgag aaattcacgc aaagccggaa ctgcctgcgc
    30541 catccccacg ccaccgccaa cgtacagcat cggtttttgc gcttttgcca gcatctggcg
    30601 cgcttgctcg acttcggcat gtgggaaagt cacttcgttt tcaacggtgg tgaaccacgg
    30661 ttccagatcg ccgctggcta actggatatc ttttgggatg ccgctggcta actggatatc
    30721 ttttgggata tcgaccagaa ccggaccagg acgacctgag ctggcaacgt cgaatgcttc
    30781 agccatgatg cgcggcaact cttccagaga ctgcaccaga aagctgtgct tggtacaggc
    30841 taacgacaat cccaggacat ccacttcctg aaatgcgtcg gtgccgataa acggtgcgga
    30901 cacctgacca gtgatggcaa cgacagggat ggaatctaac agtgcgtccg caagcccggt
    30961 tatcaggttg gttgcgcccg gaccagacgt ggcgatacat acgccagttt taccggtagc
    31021 acgggcataa ccgatagccg ctattgccgc accctgctca tgtcggcaca gcaagtgctc
    31081 cacgccgccg tcatacaatg catcgtaaac cggcataatt gcgccacccg gataaccgaa
    31141 aacggtgttc acaccctgtg cccgcaacgc atgtaccacc cactgtgcgc cattcatagt
    31201 tagttccccg tcctgaatct tgagaaacag aattttgtgc tgttattcat tgtctgctcc
    31261 tcggttatgt ttttaaggtc aaaaaaaccc ccggaccttt cggtgcgggg gtcttagttc
    31321 gttaaggctt gatctctaag cctttcctcg tccaagtgca gccccgcacg gtgggataat
    31381 aatcaccacc acgctaatca cgaccaggct aatcactcgt agaagggctg tcattttgtc
    31441 ttttcttgca tcttgttcga aggaatgcct aaagagttac catagatttt gcgaatagca
    31501 caagatattt ttgggcctat ttttatgatg gctttaataa tttcaataga aattgttgtt
    31561 ttttaaaaag aaaatagtga gtgaaaaaat tcaatttcag tataaatgtt gttcccttcc
    31621 tcgtagttca tcctttgtcc gctgcatttt tgcgagcatc attccaccgc cagaacaact
    31681 caacttcact tgcacaaata aacaggaatc agccactaaa attcacgaat tagcgggcta
    31741 cagcagcgat atcttatgca cataatggtg actcaaggag ggtttatgtc actgtcaatt
    31801 gttcataccc gcgcagccct ggaagtaaat gcgcccccaa tcactgttga ggtacatatc
    31861 agtaaaggtc tacccggctt aacgatggtg ggcttaacag aaacaacggt gaaagaagct
    31921 cgcgatcgcg tgcgcagcgc cattatcaat agcggatatg aatatccggc gaaaaaaatc
    31981 accatcaacc ttgctccagc cgatctgcca aaagaagggg gacgatatga tttacctatc
    32041 gccattgcgt tgctggcggc ctcagaacag cttacagcca ataagttaga tgaatatgaa
    32101 ttagtcggag aactagcgct tacaggcgct ctgcgtggcg ttcccggtgc aatctccagt
    32161 gcaactgaag ctattaagtc gggcagaaaa attatcgtcg cgaaagataa cgaagatgaa
    32221 gtggggctaa ttaacggtga aggatgcctg atagccgatc atctgcaggc tgtctgtgcg
    32281 tttctggaag gtaagaacac tctcgaacgc ccgaaaccaa ctgatgcagt atcccgggcg
    32341 ctacaacatg atctcagtga tgttgtcggt caggagcaag gaaaacgagg actggaaatt
    32401 accgccgctg gtgggcacaa ccttttactg attgggccgc cgggaacagg taaaacaatg
    32461 ctcgccagcc gtattaatgg cctgttgcca gatttaagca atgaagaggc actggagagc
    32521 gctgcgatat taagtctggt aaatgctgaa tcagtacaaa aacaatggcg gcagcgcccg
    32581 ttccgctcac ctcatcacag tgcgtcgtta actgcgatgg tgggcggtgg tgcaattcca
    32641 gggccaggtg aaatttcgtt ggcgcataac ggcgtgcttt ttcttgatga gctacctgaa
    32701 tttgaacggc gtacactgga tgccttgcga gagccgattg aatccgggca gatccatctt
    32761 tcacgcacac gagcaaaaat aacctatcca gcccgtttcc agcttgttgc ggcgatgaat
    32821 cccagcccta ccggacatta tcagggaaac cataaccgct gcacgccaga acagacatta
    32881 cgttatctca accggctctc ggggcccttt ctcgaccgct tcgatctctc actggagatc
    32941 ccattaccac cccccggcat tttgagtaaa acggtagtgc cgggagaaag cagcgccacc
    33001 gttaaacaac gcgtaatggc cgccagagag cgccaattta agcggcagaa taaactgaat
    33061 gcctggctgg atagtccgga aatacgccaa ttctgcaaac ttgagagcga agatgcgatg
    33121 tggttggaag aaacgctgat ccatctgggg ttatcgattc gtgcctggca gcggttactg
    33181 aaagttgcac gaaccattgc tgatattgat cagtctgaca ttatcacacg tcagcatttg
    33241 caggaggcag ttagctatcg tgcgattgac cgtttgctca tccatctgca aaaactactg
    33301 acataaaaaa agggcatttc gcccttttta ttaatcgtca gaatcggtgt agtcttcagc
    33361 accttcaacc tgcggtttac cgccggaaag ggtgtgaaaa cgttttggac gcttaatacg
    33421 cgtcatatac ttggaccaca cgcgttctgc ttctgtcact ggctcacgtt cgccacggca
    33481 tactgctacg aagagtttct cttcctcggt aaccggctcg cgtttgccaa gatccaactc
    33541 attgaaggca taaccatgac gctcaagcag ttgtgcctct ttgatggtga aatcaccatg
    33601 acgagagaat ccacgtggat aatgtttatt gtcgaaatat cgattagtcg tcgtaaagct
    33661 ttccgccatc ctgcacgctc ctaattcttt gaccgagcta gttatggcgc ggagtattag
    33721 ttacgcttga cagagtgtaa aacaaaacat ttaaatcata acgacaaata attttgcgga
    33781 gagcactgtg gatacggaat tgttaaaaac tttcctggaa gttagccgaa cgcgtcactt
    33841 tggtcgagcg gctgaatcgc tctatctgac ccagtcagca gtgagctttc gaatcagaca
    33901 actggaaaat caactgggtg tgaacctttt cacccgccac agaaacaata tccgtttaac
    33961 cgctgccggt gaaaaactac tgccttatgc agaaacgctc atgagcacct ggcaggccgc
    34021 ccgcaaggag gtggcgcata cctcacgaca taacgagttt tctatcggtg ccagcgcctc
    34081 gttgtgggaa tgtatgctta atcagtggtt gggacgcttg tatcaaaatc aggatgccca
    34141 tacaggctta cagttcgaag cgcgaattgc ccaacggcag tctctggtaa aacagctgca
    34201 tgaacgccag cttgatcttc ttattaccac tgaagcgccc aaaatggacg aatttagtag
    34261 tcagttgctg gggtatttca ctttagcgct ttataccagt gccccttcta aactaaaggg
    34321 agatcttaac tatctgcgac tggagtgggg gccagatttt caacagcatg aggcaggttt
    34381 gagcggtgct gacgaagttc ccattctgac aaccagttct gctgaactgg cacagcaaca
    34441 gattgcgatg cttaatggtt gcacctggct acccgtcagc tgggcgcgta aaaaaggcgg
    34501 cctgcatacc gttgtcgata gcacaacact ttcacggccg ctttatgcca tatggctgca
    34561 aaatagcgat aaaaatgcgt tgattcgcga tcttttgaaa attaacgtgc tggatgaagt
    34621 gtattaatct gaatggctgg caaggatgcc ggtagaagga tttacttcgg agaggattat
    34681 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggcaggg gcggagagac
    34741 tcgaactccc aacacccggt tttggagacc ggtgctctac caattgaact acgcccctaa
    34801 ttag
//
LOCUS       DADMEQ010000030        34266 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.030, whole genome shotgun sequence.
ACCESSION   DADMEQ010000030 DADMEQ010000000
VERSION     DADMEQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34266
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.030"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(94..2190)
                     /locus_tag="JNZ05_001509"
     CDS             complement(94..2190)
                     /locus_tag="JNZ05_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY8564786.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRLAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
     gene            complement(2192..2443)
                     /locus_tag="JNZ05_001510"
     CDS             complement(2192..2443)
                     /locus_tag="JNZ05_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY8564787.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            2596..3159
                     /locus_tag="JNZ05_001511"
     CDS             2596..3159
                     /locus_tag="JNZ05_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage CI repressor"
                     /protein_id="HAY8564788.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            complement(3435..3857)
                     /locus_tag="JNZ05_001512"
                     /pseudo
     CDS             complement(3435..3857)
                     /locus_tag="JNZ05_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
     gene            complement(3857..4450)
                     /locus_tag="JNZ05_001513"
     CDS             complement(3857..4450)
                     /locus_tag="JNZ05_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY8564789.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTIIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            4597..5004
                     /locus_tag="JNZ05_001514"
     CDS             4597..5004
                     /locus_tag="JNZ05_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY8564790.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            complement(5124..6116)
                     /gene="rpoS"
                     /locus_tag="JNZ05_001515"
     CDS             complement(5124..6116)
                     /gene="rpoS"
                     /locus_tag="JNZ05_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HAY8564791.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(6179..7318)
                     /gene="nlpD"
                     /locus_tag="JNZ05_001516"
     CDS             complement(6179..7318)
                     /gene="nlpD"
                     /locus_tag="JNZ05_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HAY8564792.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKIGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGELSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(7458..8084)
                     /gene="pcm"
                     /locus_tag="JNZ05_001517"
     CDS             complement(7458..8084)
                     /gene="pcm"
                     /locus_tag="JNZ05_001517"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HAY8564793.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(8078..8839)
                     /gene="surE"
                     /locus_tag="JNZ05_001518"
     CDS             complement(8078..8839)
                     /gene="surE"
                     /locus_tag="JNZ05_001518"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HAY8564794.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSSASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(8820..9869)
                     /gene="truD"
                     /locus_tag="JNZ05_001519"
     CDS             complement(8820..9869)
                     /gene="truD"
                     /locus_tag="JNZ05_001519"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HAY8564795.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(9866..10345)
                     /gene="ispF"
                     /locus_tag="JNZ05_001520"
     CDS             complement(9866..10345)
                     /gene="ispF"
                     /locus_tag="JNZ05_001520"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HAY8564796.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(10345..11055)
                     /gene="ispD"
                     /locus_tag="JNZ05_001521"
     CDS             complement(10345..11055)
                     /gene="ispD"
                     /locus_tag="JNZ05_001521"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAY8564797.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(11074..11385)
                     /gene="ftsB"
                     /locus_tag="JNZ05_001522"
     CDS             complement(11074..11385)
                     /gene="ftsB"
                     /locus_tag="JNZ05_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HAY8564798.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(11579..11902)
                     /locus_tag="JNZ05_001523"
     CDS             complement(11579..11902)
                     /locus_tag="JNZ05_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HAY8564799.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(11952..12557)
                     /gene="cysC"
                     /locus_tag="JNZ05_001524"
     CDS             complement(11952..12557)
                     /gene="cysC"
                     /locus_tag="JNZ05_001524"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HAY8564800.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVAAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEEPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(12557..13984)
                     /gene="cysN"
                     /locus_tag="JNZ05_001525"
     CDS             complement(12557..13984)
                     /gene="cysN"
                     /locus_tag="JNZ05_001525"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HAY8564801.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(13986..14894)
                     /gene="cysD"
                     /locus_tag="JNZ05_001526"
     CDS             complement(13986..14894)
                     /gene="cysD"
                     /locus_tag="JNZ05_001526"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HAY8564802.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15146..16183
                     /gene="iap"
                     /locus_tag="JNZ05_001527"
     CDS             15146..16183
                     /gene="iap"
                     /locus_tag="JNZ05_001527"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HAY8564803.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(16269..17420)
                     /locus_tag="JNZ05_001528"
     CDS             complement(16269..17420)
                     /locus_tag="JNZ05_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HAY8564804.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKNRRGEKEGSDDYHLLCWHLLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     EFKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPRIEIPTFWDDDEGVELIKQLSW
                     YISATVVLADWTGSSTRFFPRVAQAMDIEDYWQKALVQAQNALTVFPPQAETAPFTGI
                     NTLFPFIENTTPLQQKVLDLDINQPGPQLFILEDVTGAGKTEAALILAHRLMAAGNAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPEVFPAPAEINRRNTVRFSRK"
     gene            complement(17614..17766)
                     /locus_tag="JNZ05_001529"
     CDS             complement(17614..17766)
                     /locus_tag="JNZ05_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY8564805.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(18030..18764)
                     /gene="cysH"
                     /locus_tag="JNZ05_001530"
     CDS             complement(18030..18764)
                     /gene="cysH"
                     /locus_tag="JNZ05_001530"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAY8564806.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(18838..20550)
                     /gene="cysI"
                     /locus_tag="JNZ05_001531"
     CDS             complement(18838..20550)
                     /gene="cysI"
                     /locus_tag="JNZ05_001531"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HAY8564807.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(20550..22349)
                     /gene="cysJ"
                     /locus_tag="JNZ05_001532"
     CDS             complement(20550..22349)
                     /gene="cysJ"
                     /locus_tag="JNZ05_001532"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HAY8564808.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            22665..23030
                     /gene="queD"
                     /locus_tag="JNZ05_001533"
     CDS             22665..23030
                     /gene="queD"
                     /locus_tag="JNZ05_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HAY8564809.1"
                     /translation="MMSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGE
                     VDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPL
                     LSAVMVKETCTAGCIYRGE"
     gene            23108..24379
                     /locus_tag="JNZ05_001534"
     CDS             23108..24379
                     /locus_tag="JNZ05_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY8564810.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            24370..24630
                     /locus_tag="JNZ05_001535"
     CDS             24370..24630
                     /locus_tag="JNZ05_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY8564811.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GGLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            24647..25222
                     /locus_tag="JNZ05_001536"
     CDS             24647..25222
                     /locus_tag="JNZ05_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HAY8564812.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEVF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(25370..26263)
                     /locus_tag="JNZ05_001537"
     CDS             complement(25370..26263)
                     /locus_tag="JNZ05_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAY8564813.1"
                     /translation="MAGLFTPKDAAMNIAIVTINQENAAIASWLAAQDFSGCTLAHWQ
                     IEPQPVVAEQVLDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLD
                     IPTVSMRKSHWGNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPD
                     WLVSTEDLKNVSRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGTEVGYSRARVMNGG
                     VDAEKVIGISGHLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADID
                     VVDDWKVVLEALVTNIHAEYQ"
     gene            complement(26227..27006)
                     /locus_tag="JNZ05_001538"
     CDS             complement(26227..27006)
                     /locus_tag="JNZ05_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAY8564814.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRCGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(26984..28393)
                     /locus_tag="JNZ05_001539"
     CDS             complement(26984..28393)
                     /locus_tag="JNZ05_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8564815.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(28415..29853)
                     /locus_tag="JNZ05_001540"
                     /pseudo
     CDS             complement(28415..29853)
                     /locus_tag="JNZ05_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            complement(29923..30709)
                     /locus_tag="JNZ05_001541"
                     /pseudo
     CDS             complement(29923..30709)
                     /locus_tag="JNZ05_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            31027..32304
                     /locus_tag="JNZ05_001542"
     CDS             31027..32304
                     /locus_tag="JNZ05_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8564816.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            32331..33809
                     /locus_tag="JNZ05_001543"
     CDS             32331..33809
                     /locus_tag="JNZ05_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY8564817.1"
                     /translation="MSKKYIIGIDGGSQSTKVVIYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHILFDVQMPGTVLGHITPQGALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         8275 a         9073 c         8426 g         8492 t
ORIGIN      
        1 atcatcaata tcagtcatag tatttcctcg gttatttcaa atcagctttg tttcctttat
       61 ttaacagtcc gttcccgcgg actgtttttt tattcaggcc agcggatcat cttcttcctg
      121 catggcaaaa agtttttgca tccccagccg gaaatcgcgt tcaaattctt cgtcatgctc
      181 ttcttcatat tccggttgct gaactgccac cacattacca accgggcgat aaacattact
      241 cagccagggt tcagcctgtt tgtgttccag aatctgaata ccttcatctt cagcatcacg
      301 aattttttcc tcagcgcgtt tgcgcattcc tttaatacgc tgctgttgtt tgtgatattc
      361 agcactgaca gcaaaggctg cgcgtttatt gccgttccat atcgcctcac agataaagct
      421 gccgtccttg cgacgtaccg taattcgctc ggcgtcatga atgtcataac tgataagcac
      481 tttgcgaccg tgttcgtcac gcaactccgg cgcgtagtaa atattgttca gccactgaat
      541 ttcgcagcgt cttaccggtc gttccaccat tgggcggaac atgtcgcgca gctcaagatc
      601 actcagccac tcaatttcgg tgtcttcttc tgccaggcgt ttttttctga actctgtcgg
      661 gctgtaatgc cgtccgttcg gtttcatcgg cagctcagaa tgcgggcggt tgttgtacca
      721 ttccacgcct tcacgaatag cctcaataag ctccgcccac gatggcagct cgcgcattgc
      781 cgctttctgg cggtcgttca gaggcttgtc ctgttgcagg gcgttaaacg ctgaacgcag
      841 atcacggtta agtacacgca acgattcccg gtcagcaccc ttgccaaaat aggtgcgata
      901 cctcctggct atacgcatcg gcagtgtccg gttcaggcgt tcaataatgc cgcgtccctg
      961 tggattaccg gcaataccgg tagggtgttt aatccccagg cgtggcagaa tacccacgac
     1021 ctctttgtcc agcatgtcag ccgtttcacc ggaaccgtta tccgagtaat acaggaacgg
     1081 ttttccgtgg ttgcgaatgc cgtgctgtat cgcaccggca acggcgaaca cgttttcggc
     1141 aaggtcaaga ctccagccaa caacaaaccg tgttccacca tcgatcacaa acgtgacttc
     1201 cggtgagaat ggacgcccgt ggatcggatg cgcacatttc agcttcatac cgtgaccgtc
     1261 accaatccag acataattca ccggcatagc tgtccagtcg cggcgggtga atccttcaag
     1321 ctgacggtat tcactgccgg taacgcgccc tttttgtttc accacttccg gcagtttttt
     1381 catggcatac cggacaacat cataggaagg catcacctcc agcatgtacg gttcgtccgc
     1441 atggcgacgc tgccattctg cgacaaaatc ctcgtaggct tctgacatgg gtctgccgtt
     1501 cacctggcga tactgcgcca gaaattcagg cagccagctg atttcttccg gttttatttc
     1561 gtcgcgtttt cccggtgcca gtaacagcag ccgttcggcg gcgttctgcg actggttgta
     1621 tttcagaacc cactggtaaa gtggatcgcg gctgatagta cgattagcgc cacgttttgc
     1681 attggcgtta actgcggcct gttgcaggcg ctccggcagc tcacctttac gggctttttc
     1741 aacaagaaag ttaatggctt tcagtcgccc gaggttaccg gcgctttcca gcttcagcac
     1801 ttccaccacc agcgcagctc ttgcttcaga tacccagcgc tggttttcgg tcagcgcatt
     1861 caggcgttct tccatcagtt gtggagaacc gcgatatgct tcgattgcct ggacagcgtc
     1921 aggattgcgt gtttttctgg taacagcttt cacttcagaa acattgttct ctgtagccac
     1981 cagctgttcc acatagcgtt cacgcagtgc ctttcgtgtt atttcaggca gacaatcaat
     2041 gtggtactca atggcttttg tgccttcccg acggcgacta acgtcaccaa gatcacgaga
     2101 aaaacgctgt aacgcctgtc ggatgccttt tgtggtggct ggcattcctg gtacgccgag
     2161 caggtcattt acggtaacgt acgactgcat atcacgacgc cttcctgata aatccatcgt
     2221 tacgataccg gctgggccag atgatttctg gcggtacacc tatagcgtca gaaattacgc
     2281 gttcgtattt tttgtttggt gtgcgaacta ccgctttcag tgaatcctga gcataaccag
     2341 catggagtga aagctcccgg aaggaagacc acccgttatc tttgagcgcc agctgaatct
     2401 tcagcgaagg ccaatcgcgg tcactatctt ctctttgtct catctttcct ttaacctaca
     2461 aaataacccg cgcggatatc cgtatggata aagattaaga taccaaagat tccctataaa
     2521 gggttctttg gtgcttttat ggtttatttt ttaatttcat tatttatcaa tgaattagat
     2581 agaaaggaac caagaatgag aaaagcaccc caaggttctt ttcctgccga tggaaaagaa
     2641 ccgattatcg aaagaatctt taggttagta gaacgatacc cttcaagaaa tgcggctgct
     2701 cgggcatggg ggatcaatga aggcacttta aaaaattatt acaacaggcg ggattacgca
     2761 ccgataccac gcccgcatca gttaaaaaag atagcagaaa gcgaaaatgt taccttggaa
     2821 tggctcatga cgggagaggg tgatgttgaa ctaaaagatt ccaaagaacc caaaaaagag
     2881 aatgcggata atgttgatgc tcaaattcta atgttcttgt catttcttaa accacaagaa
     2941 aaacaacaag tagctgatgt tcttgggaga aaaggagcgg agcaattact cattcttctg
     3001 gatgagcaca tccaagagtt gcacgctcta gttggagttc gtagagccat agcgttaagc
     3061 cttggtaatt tgccagacga aaaggttaga gagatttacg cgttgtccga agctaagggc
     3121 gatcacttaa atctacctca gaaagcagcc agcgcataac ccagtgcaag tgcctagctg
     3181 tgctaaaatt acattggttt tggcgcttgc acccatcagc gctataataa acacctgaat
     3241 cgtttttgaa cggtctttaa aggctattgt aagaacctgt gagttatctt gaatgtttta
     3301 ttgatatcga accgtttaac atttttggac tcgctttatc ttgaaaacct tgagttccta
     3361 acttgttgat tccaatctat tcccgtcttt tttaatctct tcccggttat cttgaataaa
     3421 ttacctagtc atacgagata gaaaccgcca tcgccatcat tcaggcgaaa cagcggcaga
     3481 atatcgaaca ggttgatctc ttcgccatcg acggtgttct gcgcccaggc tggattggcg
     3541 cggcgctccg gggcaatcgg gaagttatcc cagcggcgga taaactcatc aatctgcttt
     3601 ttgaccgggg tgtttggtgg caggccaagg gaaatcgcgt ggttctgcca ggaaccgatg
     3661 gtattcatcg ccacgcgggc atcggtaaag ccgcgaatat tatcaaacca cagcgcgggc
     3721 gcgccgtcgc cgatacgccc ggtggcgtta gctgctgctg ccagatccgg ctcagcgttc
     3781 acttcttcgc tgattttcag taactggccg tggtcatcaa gcgcctgtaa aaagctgcgt
     3841 aaatcatcaa atgccattat tcattctcct gagaaaaatt ccgggcctgc ggcaatcctt
     3901 gccagcgcct ggcgtgggga tgttcgaggc caaattgatc cagcacgcgg gctaccacat
     3961 ggtggacaat gtcatctacc gtttcgggat ggttataaaa ggcaggcatc ggcggcacca
     4021 tcgccacgcc catgcgtgaa agtgcgagca tattttcgag atggatggtg ctaagcggca
     4081 tttcacgcgg caccagcacc agtttgcggc cttctttgag cacgacgtcc gccgcgcgcc
     4141 ctaccaggcc atcggcgtaa ccagcgcgga taccggcgag cgttttcata ctgcacggaa
     4201 taacgatcat gccgtcagta cgaaaagaac cggatgagat gatcgccgcc tgatccgccg
     4261 ggttatggct gaagtctgcg agggcagcaa catcgcgagc gctgtaaggc gtttccagtt
     4321 caatgatggt tttcgcccac ttcgacatca ccagatgagt ctcgacattc ggcatctcac
     4381 gcagcgcttg cagtaatgcc acaccaagag gcgcaccggt agcccctgtc atcccgacga
     4441 tcagtttcat tgcttattct cttaattgtt cgtatacgaa cgttattatt aacatactcc
     4501 ttcatttcca cagcggcaag gattgaaaag taacaacatc ttcttcgtca ctaaaaaacc
     4561 gctatgatag cgacagagaa ttagcccgga gtttacatgg cgttacgaaa taaagcgttt
     4621 catcagttac gacagctttt tcagcagcac acggctcgct ggcagcacga gttacctgac
     4681 ctgaccaaac cacagtacgc ggtgatgcgc gccattgctg ataaacctgg tattgaacag
     4741 gtggcactga tagaagcagc agtcagcacc aaagcaacgc tagcagaaat gttggcaaga
     4801 atggaaaatc gtggcttagt cagacgtgaa catgacgtcg ctgacaagcg gcgacgcttt
     4861 gtctggctga ctgccgaagg agaaaagcta cttgctgcgg cgatacctat tggtgacagc
     4921 gtggatgagg aatttttggg gcgtttgagt ggagaagagc aggagctgtt tatgcagttg
     4981 gtgcgcaaga tgatgagcac atagaatgct gattgccggg taggacgctg acgtgtctta
     5041 tccaggcgac aaaaacacaa gcttcccaaa caaaagaaaa aggccagcct cgcttgagac
     5101 tggcctttct gacagatgct tacttactcg cggaacagcg cttcgatatt cagcccctgc
     5161 gtttgcagga tttcgcgcaa acggcgcagg ccttcaacct gaatctggcg aacacgttca
     5221 cgggtgaggc caatttcacg acctacatct tccagtgttg ccgcttcgta ccccagcaaa
     5281 ccgaatcgac gtgccagtac ttcacgctgt ttggcgttca gctcgaacag ccatttgacg
     5341 atgctctgct tcatatcgtc atcttgcgtg gtatcttccg gaccgttctc tttttcatcg
     5401 gccaggatgt ccagcaacgc tttttcggaa tcaccaccca gcggggtgtc taccgaggta
     5461 atgcgctcgt taagacgaag catacggctg acgtcatcaa ctggcttatc cagttgctct
     5521 gcgatctctt ccgcacttgg ttcatggtcc agcttatggg acaactcacg tgcggttcgc
     5581 aggtaaacgt tcagctcctt tacgatgtga atcggcaaac gaatagtacg ggtttggttc
     5641 ataatcgccc gttcaatcgt ctggcgaatc caccaggttg cgtatgttga gaagcggaaa
     5701 ccacgttccg ggtcaaactt ctctaccgcg cggatcagcc ccaagttgcc ctcttcgata
     5761 aggtccagca acgccagacc acgattgcca taacggcggg caatttttac caccagacgc
     5821 aagttactct cgatcatccg gcggcgagag gcgacatctc cacgcagtgc gcgacgcgca
     5881 aaataaactt cttcttcggc cgttaacagt ggtgaataac caatctcacc aaggtaaagc
     5941 tgagtcgcgt ccaacacacg ctgtgtggct ccctgcgata acagttcctc ttcggccaaa
     6001 tcgttatcac tgggttcctc ttctactaag gccttttcgt caaaaacctc aactccgttc
     6061 tcatcaaatt ccgcatcttc atttaaatca tgaactttca gcgtattctg actcataagg
     6121 tggctcctac ccgtgatccc ttgacggaac attcaagcaa aagcctggtt ccgccgattt
     6181 atcgctgcgg caaataacgc agcgggttta cggatttccc cttgtaacga atttcaaaat
     6241 gcaagcgtgt tgaactggtt ccggtgctac ccatggttgc tattttttgc cccgccttca
     6301 cttcttgttg ttcccggacc agcattgtgt cgttatgggc gtaggcactc aggtaatcat
     6361 cattatgttt gatgataatc agattaccgt agccgcgcag cgcgttacca gcataaacaa
     6421 cgcggccatc tgcggtcgcg ataattgcct gtcctttgct gcctgcgata tcaatcccct
     6481 tgttgccccc ctcagaagcg ccaaaggttt cgatcacttt gccctcagtc ggccagcgcc
     6541 aggtggagat aggcgtactg gttgatgtac tgctgacagt cggctcggtt gtgcttgctg
     6601 ttggtaccgt tacaggcgct gtgaccgtgg tcgcagttgg cttgttgttc ggcaacattt
     6661 tgttagcact cagttcaccc gaagactcag aatacgtaat tgtcggttgc gacgcaacag
     6721 caacggtgga attttgtgca ggcttgatca caactccttg ctctgctgcg tcggcctggg
     6781 taatagcatt tccgccagtg attggcgtac cggaagcatt acccacctgc aaggtctgac
     6841 caacgttcag cgcgtatggt gcctgaatat tgttgcgctg agcaaggtca cggaaatcgt
     6901 tgccagtaat ccaggcgata tagaaaagtg tgtcaccttt tttcacggta taggtactgc
     6961 cgctataact gcctttcgga atgttcccat actgacggtt atagacgatg cgtccgtttt
     7021 ccatctgtac cggctgctga gctactggct gcactggctg gatttgcggt tgttgagtag
     7081 cctgaatttg tggctgctgt accggctgaa tttgcggttg ctgcgctgta gacgtcgtcc
     7141 ctattttcgg cggcggcgta atcaacatac cagaattagt atttgcaggc gcattgccat
     7201 taacggagct gaccggtgcc ggtggatttg aagtgtcaga acagcctgcc agccatagcg
     7261 aaaccagtga caaagccgca atgcggcgaa cggtgaattt tgggcttccc gcgctcattt
     7321 atcccccagg aaaaattggt taataaccag tgacataatt accgtgcaag gcaccctact
     7381 gaacactgga aaagatgttc acgatacgct gacctacggc aaaataacca ggaaaaatcc
     7441 aggtatttcc tcacgtttta agccagctca cccttcacta aagggacaaa gcgcacggcc
     7501 tccacggtat cgataataaa ttcgcctccc cgacgacgca cccgtttcaa atactggtgc
     7561 tcctccccta cgggtaagac gagaatcccg ccttcgtcca gctgcgtcat tagcgcagtt
     7621 ggaatttccg gcggtgccgc cgtaacaatg atagcgtcaa acggcgcacg tgcctgccaa
     7681 ccttgccatc catcaccatg acgggttgaa acattatgta aatcaagatt tttcaggcgg
     7741 cgacgtgcct gccactgcaa gcctttaatc cgttcaaccg agcaaacatg ctggacaaga
     7801 tgcgccagga ttgccgtttg atatcccgaa ccagtgccaa tttccagcac ccgcgactgc
     7861 ggcgtcagct cgagtaattc ggtcattcgc gccaccatat acggctgcga aattgtctgc
     7921 ccctgaccta tcggcaaggc gatattgtcc caggcttttt gttcaaacgc ttcatcaacg
     7981 aatttttcac gcggcacggc ggcaagtgca ttcagcacct gctcatcctg aataccttgc
     8041 gcacgtaatt gatccagaag tgcttgtacg cgtctgctta ccattgcgtg ccaactccca
     8101 cgctgtttaa ccagtctgaa accacatctt gcgcgctatg cgcagttaaa tccacatgca
     8161 gcggcgtgat ggagacatag ccctcatcta ccgcagcaaa atcggtcccc ggaccggcat
     8221 cacatttacc gcccggcggg ccaatccagt acagcgtatt gccgcgcgga tcttgctgcg
     8281 ggatcacctg atctgccgga tgtcgtgtac cgcagcgcgt cacgcgaata cctttgattt
     8341 gatccaaggg taaatccgga acgttaatat taagaatacg tccggtgcgc agcggctctt
     8401 tacacagtgc gcgcaaaatt gaacaggtta ccgccgcggc agtgtcgtaa tgtttatgcc
     8461 cgtcaagcga gacggccagc gccggaaaac ctaaatgacg gccttccatc gcggcggcta
     8521 ccgtaccgga ataaataaca tcatccccca gattcggccc ggcgttaatt ccggacacaa
     8581 caatgtccgg gcgcggacgc atcagagcat tcacgccaag atagacacaa tcggtcggag
     8641 ttcccatttg cacagcaata tcaccatttt caaaggtaaa cgtgcgcagg gaggattcca
     8701 gtgtcagaga atttgaagcg ctgctgcggt tacgatcggg ggcgaccacc tgaacgtcag
     8761 caaactcacg caaggctttc gccagcgttt gtataccggg tgcatgtacc ccgtcatcat
     8821 tactcagcaa tatgcgcata atcacctgtt gtgttgataa gttcccttac aacgctggtt
     8881 gcaaaactac ccgccggaag ccagaaacgg atctctacgg tgacgtcatc ccaccagttc
     8941 cagcttaatt gttgcggata cagcagcatc gctctgcgcg cggcttcaac tttttcgcgc
     9001 accagtaaag cttgtaattc agtttctgcg gcgacagctg cttgttcgaa tgccagcgct
     9061 tcacgctgag ttccccattc gccactgccc ggcaatgcgg cggttatcat cagctcttta
     9121 tcgttgacgc gacgctgtaa ttccgccagt tcttcggtgg ttgctacaaa ccagctacca
     9181 cgtccggcta attgtagcgc atcgccgtca acaacttgat taacgtctgc ttttttgagg
     9241 cgctcagcaa caatctgatt aaacaacgca ctgcgggctg ccgacaacca aaaactccgt
     9301 ttattgcgat cgcgcaccgg agtattggtt tgcgcccagc gcagcgcgcc ctgcaagttg
     9361 ctaccgccaa tcccaaaacg ttgggcaccg aagtagttcg gtacaccttt tacgcaaata
     9421 tcgatcagac gttgttcaac gtcatcgcga ttgctcactt cgcgcagaac cagggtaaag
     9481 gcgttacctt tcagcgcccc taaacgcagc ttgcgcttgt gccgcgcata ctccagcacc
     9541 tggcagcctt ccagttgaaa ggcgctcaga tcgggcattt ccttgcccgg cacgcgagca
     9601 cataaccact gttccgttac agcatgtttg tctttttgcc cagcgaagct gacttcacgg
     9661 gaatgaattt tcaggaattt cgccagtgca tccgccacaa aacgggtatt gcagccgttt
     9721 ttgaggattc taaccagaat atgctcacct tcaccatcag gctcaaagcc caaatcttcc
     9781 accaccacaa agtcttccgg attggctttc agcagcccgg tgccttgcgg tttaccgtgg
     9841 aggtaagtga gattatcaaa ctcaatcatt ttgttgcctt aatgagtagc gccaccgctt
     9901 cacaggcaat cccttcccca cgtccggtaa atccaagttt ttccgtagta gtggctttca
     9961 cgttaacatc atccatatgg cagccgagat cttcggcaat aaacacgcgc atttgtggaa
    10021 tgtgcggcaa catcttcggt gcctgagcga tgatagtgac atcgacgttg ccaagggtat
    10081 aacccttcgc ctgaatacga cgccaggctt cgcgtagcag ctcgcggctg tcggcacctt
    10141 taaatgccgg atcggtatcc gggaacagtt tgccgatatc ccccagcgcc gccgcgccaa
    10201 gcaatgcatc ggtcaacgca tgaagcgcca cgtcgccatc agaatgcgcc agcaatcctt
    10261 tttcgtaagg aatgcgtacg ccaccaatga taattgggcc ttcaccgcca aaggcgtgta
    10321 cgtcaaaacc gtgtccaatt cgcattatgt attctcctga tggatggttc gggtgaggta
    10381 aaactcggcc agtgccaaat cttccgggcg cgtgacttta atgttatccg cgcggccttc
    10441 gaccaactgc ggatgaaatc cgcaatattc cagcgccgag gcttcgtcgg taatggtagc
    10501 gccttcattt agagcgcgcg tcagacagtc atgtaacagc tcacgaggga aaaattgcgg
    10561 cgtcagcgcg tgccataagc cgttgcgatc aacggtatga gcaatggcat ttttgcccgg
    10621 ttcggcacgt ttcatagtat cgcgcactgg tgcggcgagg atccctcccg tgcggctggt
    10681 ttcgctcaac gccaacaatc gcgcgaggtc atcctgatgc aaacaaggac gagcggcgtc
    10741 atgcaccaat acccactgca catcgccagc ggctttcaga cctgccagca cggaatcggc
    10801 acgctcatca ccgccatcta caacggtgat ttgcggatga ttcgccagag gaagttgtgc
    10861 aaaacggcta tcgccaggac ttatggcaat gacgacacgt gtcacccggg gatgcgccag
    10921 cagcgcatgc accgagtgtt caagaatggt ttgattaccg attgagagat attgcttagg
    10981 acattccgtt tgcattcgac ggccaaatcc ggccgccgga accacggcgc aaacatccaa
    11041 atgagtggtt gccatgttaa ttcccgggct gatttatcga ttgttttgcc ccgcagactg
    11101 tgcgcgcttc gacgcgtcag gcaccagacg ataaaaagtt tcgcccggcc tggtcatgct
    11161 gagttcatta cgcgcacgct cttcgagcgc ctcctggccg ccattgagat cgtcaatttc
    11221 ggcaaaaagt tgatcgtttc gcgctttaag tttcgcgttt gtagcttgca gtgccgccac
    11281 atcatcattg acgcgggtat agtcatgtat accgttctta ccgaaccaca gcgaatactg
    11341 tagccagacc agaatagcca gcaacagcag cgttagttta cccatcctgc cccctgaaaa
    11401 actgcatcat catcccatgc atccgaagac gactctacat cctctgttgg ggataccgcg
    11461 acaacgcggg caaatgtacc acatttgtcc attgttacgt atacccaggg cgtgcagaac
    11521 ataatctcat tattagttac ggtttgaatt atgaacagag gagacaagaa agtacaaatt
    11581 agcccagtag ccacataaac agtgcgccaa acataatgcc tactgtcacc agggtgaaaa
    11641 caatactgta gcgtagcttt ccgtccatca atgaatgcag cgcaatcccc accactaccg
    11701 cgacaggcat cagtgccaga aagaaaggcc aggtgtagat aaagaagaac agcgtgttag
    11761 agccataaat caacatcggc atcgccagcg caaataacca ggagataaaa ccgaccacgg
    11821 caccaggcag tgaccatgta gtttcttcat cctcagtaag gctgtcgtta tttgttagtg
    11881 taatgttatg gctattacgc atatttgatc ctgttacttt gacgaaccgg gcatggaaac
    11941 ccggtggtgt ctcaggatct gataatatcg ttctgtctca acagatctaa taattgctgt
    12001 accaaatttg ttactaattg ttcaccattg agatgaattt ctgccgattc aggctcttcg
    12061 taaacggaat ctattcccgt aaagttgcgc agttcaccgg cacgcgcttt cttatataag
    12121 ccttttggat cgcgggcttc gcaaatcgcc agcggcgtat cgacaaacac ttcaataaag
    12181 cgcccttctc ctacgcgttc gcgaaccatc tggcgttcgg cgcggtgtgg cgagataaat
    12241 gcggtcagca ccaccagtcc ggctgcaacc atcaaattcg ccacttcacc gacgcgacgg
    12301 atattctctt tacgatcggc atcgctaaaa ccgagatcgc tgcataatcc gtggcgaaca
    12361 ttgtcgccat ccagcagata cgtactgacg ccgagtttat gtaacgcctc ctccagcgcc
    12421 ccggcgaccg ttgatttacc ggacccggag aggccggtaa accacagcac tacaccacga
    12481 tgaccgtggt gtagttcgcg ttgttgcaca gtgaccggat gactatgcca gacgacgttt
    12541 tcgtcatgca gcgccattat ttatccccca gcaaatcgcg cgcgccccag tgcggaaagt
    12601 ggcgacgaac cagggcattc aattccagtt cgaatgcact gaattcagat gacgcagctg
    12661 ttgcctggct aactggctcg tgcaccatac cggcacccac ggtcacattg ctcaggcgat
    12721 cgataaaaat cagcccaccg gtaaccggat tttgctgata gcgatctaac accagtggct
    12781 cgtcaaaagt gagatccacc aggccgatgc cgttcagcgg caggttttca acttcgcgtt
    12841 gggtaaggtt attaatatca acctgataac gaatgccatc aacacgagca cgcgtcttct
    12901 taccggcaat tttgatgtca taactctggc ccggggaaag cggctgttcc gccatccata
    12961 ccacatccac cgacgcactc tgcaccgccg gtaacgcttc gtctgccgcc agcagcagat
    13021 caccacggct gatatcgatc tcatccgtca gcaccagggt gatcgcttct ccggcaaaga
    13081 cttcttcgcg atcaccatca aaagtcacga tccgcgcgac gtttgattcc acaccagagg
    13141 gcagcacttt tacacgttgc ccgacttcca cgcgaccgga tgccagcatt ccggcgtagc
    13201 cacgaaaatc gagatttggg cggttaacgt actgcaccgg gaagcgcatt ggctgggcat
    13261 ccaccactcg ctggatctcc acggtttcca gcacttcgag cagtgtcaga ccgctgtacc
    13321 acggcatact ttcactttgc gaagccacgt tgtcaccttc cagtgcggag agcggcacaa
    13381 agcggatatc cagattaccc ggcagctgcc cggcaaaggt cagataatct tcacgaatac
    13441 gggtgaacgt ctcttcactg taatccacca gatccatttt gttgatcgcc acgaccagat
    13501 gtttgatccc caacagtgtg gagataaaac tgtgacgacg ggtttgatcg agcacgcctt
    13561 tacgggcatc aatcagtaag atcgccagtt cacatgtcga tgcgccagtc gccatattgc
    13621 gagtgtactg ctcgtgccct ggggtgtcgg cgataataaa tttacgcttc tcggtagaga
    13681 aatagcggta ggccacatca atagtgatgc cctgttcgcg ctcagcttgc aggccgtcca
    13741 ccagcagagc cagatccagc ttttcgccct gggtgccgtg acgcttactg tcgttatgca
    13801 gcgatgagag ctgatcttcg tagatttggc gggtatcgtg cagcagacga ccaatcaggg
    13861 tacttttgcc gtcatcgacg ctaccacagg tcagaaaacg cagcaggctt ttatgttgtt
    13921 gcgcaatcat ccaggcttcg acgccgcctt cattggcgat ttgttgtgca agtacggtgt
    13981 tcatcttaaa aatacccctg acgttttttc agctccatgg atcccgcctg gtcgcggtca
    14041 atcacgcgcc cctgacgttc actggtggtg gaaaccagca tctcttcgat gatttccggc
    14101 agtgtttgtg catttgactc caccgcaccg gtcagcggcc agcagcccag cgtacggaaa
    14161 cgcaccatcc gttttttgat cacttcgccc ggttgcaggt cgatacggtt gtcatcaatc
    14221 atcatcaaca taccgtcgcg ttccagaacc ggacgttccg cagcgagata cagcggcaca
    14281 atgtcgatat tttccagcca gatgtattgc cagatatcct gctcggtcca gttagagagc
    14341 gggaagacgc ggatgctttc gcctttgtta atttgcccgt tgtagttgtg ccacagctcc
    14401 gggcgctgat ttttcggatc ccagcgatgg aagcggtcac ggaaagagta gattcgctct
    14461 ttagcgcggg atttctcttc gtcacggcgc gcaccaccga aggcggcatc aaaaccgtat
    14521 ttgttcagcg cctgtttcag gccttcagtt ttcataatat cggtatgttt cgcgctgccg
    14581 tgcacgaatg gattaatccc catcgccacg ccttccgggt ttttatgcac cagcagctcg
    14641 cagccgtagg ctttcgccgt acgatcgcgg aactcataca tctcgcggaa tttccagccg
    14701 gtatcgacat gcagcaacgg gaaaggcagc gtacctggat aaaacgcctt gcgcgccaga
    14761 tgcagcatga cgctggaatc tttaccgata gagtagagca tcaccggatt tgagaattct
    14821 gccgccacct cgcgaataat gtggatgctt tccgcctcca gttgccgcag gtgagtaagt
    14881 cgtttttgat ccataaccgt tcctttgcaa taccgctatt ttcttgccat cagatgtttc
    14941 gactataggg agcgtaagag aacgaatgaa attaccaatt agaatgagta gttccttaac
    15001 ggaataacga tttggcaaag ctaatatcaa aaagtgctta aggcaccgga tttcgggcgt
    15061 ttaggaagat ttgaaattgt tttagcgcag cggcagtttc atactatggc ggtaaaaaaa
    15121 tttgcatggt atttaaggac tcactatgtt ttccgcattg cgccaccgta ccgctgccct
    15181 ggcgctcggc gtatgcttta ttctccccgt acacgcctcg tcacctaaac ctggcgattt
    15241 tgctaatacc caggcacgac atattgctac tttctttccg ggacgcatga ccggaactcc
    15301 agcagaaatg ttatctgccg attatattcg ccaacagttt cagcaaatgg gttatcgcag
    15361 tgatattcgg acatttaata gtcggtatat ttataccgcc cgcgataatc gcaagaactg
    15421 gcataacgtg acaggaagta cggtgattgc cgctcatgaa ggcaaagcgc cgcagcagat
    15481 catcattatg gcgcatctgg atacttacgc cccgctgagc gatgctgacg cagatgccaa
    15541 tctcggcgga ctgacgttac aaggaataga tgataacgcc gcaggtttag gtgtcatgct
    15601 ggaactggca gaacgcctga aaaatacgcc taccgagtat ggtattcgat ttgtggcgac
    15661 cagcggcgaa gaggaaggaa aattaggcgc tgagaattta ctcaagcgga tgagtgacac
    15721 cgaaaagaaa aatacgctgc tggtgattaa tctcgataac ttaattgttg gcgataaatt
    15781 gtatttcaac agcggtgtaa aaacccctga agcagtaagg aaattaacgc gcgacagggc
    15841 gctggcaatt gcacgcagtc acggaatagc cgcaacgacc aatccgggtt tgaataaaaa
    15901 ttatccgaaa ggcactgggt gttgtaatga cgcagaaata ttcgacaaag cgggcattgc
    15961 tgtactttcg gtggaagcga ctaactggaa tcttggaaat aaggatggtt atcagcaacg
    16021 cgcaaaaaca cctgccttcc cggcgggaaa tagctggcat gacgtaagac tggataatca
    16081 acaacatatt gataaggcac ttccaggaag aatagaacgt cgctgccgtg acgttatgcg
    16141 gataatgcta ccgttggtga aggagctggc gaaggcgtct tgatgggttt gaaaatggga
    16201 gctgggagtt ctaccgcagg ggcggggaat tctaagtgat acccatcatc gcatccagtg
    16261 cgtccggttt acttccggga aaaccgcacc gtattgcgcc ggtttatctc cgctggagcg
    16321 gggaacacct ctggcgaata gaaagcaagc caggttttga ccagtcgatc gtacatggca
    16381 ttggccgttg ccattgttgg caggccaaaa aacagcccct gagcgttacc cgcagccatc
    16441 aacctgtgcg ccaggataag cgccgcttct gttttacctg cgccggtcac atcttccaga
    16501 ataaacaact gtggtcctgg ctgattgata tccagatcca gcaccttttg ctgtaacggt
    16561 gtcgtattct caataaaagg aaacagcgta ttaattccgg tgaaaggcgc ggtttctgct
    16621 tgtggcggaa agacggttaa tgcgttttga gcctgaacta aagccttttg ccagtaatct
    16681 tcaatatcca ttgcctgtgc gacgcgtgga aaaaatcgcg ttgacgaccc tgtccagtct
    16741 gcaagtacga cggttgcaga gatataccag gaaagttgtt ttataagttc aacgccctcg
    16801 tcatcatccc agaatgtggg gatctctatt cgcggaaaca gtggcttgat ttcaaggaga
    16861 aaatctcgtg ctgcagcttt gtcttcaggc agaaaattat ccaattcatc catacgatca
    16921 gggggccgac catgatgccc tgtcgtcacg ggcatccaca tttctatcac tcgcctaagt
    16981 ttgcgcggag agagcgaaga tgaaggaagt aactcctgac attcacttaa ataatgattc
    17041 cacaaccagt agcccagcgt tgaatgagag attttttcgt aattttttct ggcaccttcc
    17101 tgaatcttga attcaggggg caggtaaagt tgttgaaaag agcgggcaaa ttttccaata
    17161 tcgtgccagc acagcaacca ggcgaaaaat tgagctgcct gttccttgtc agaaatccct
    17221 aattgacgaa aataatcagc cagcccgaag caatttcttt taaccattaa atagcccatt
    17281 gcggccacat ccagcaaatg ccagcaaaga aggtgatagt cgtcgctacc ctctttctcg
    17341 ccacgtcggt tttttcccca gaaatcaaag aaagtcacaa tatttttatc cttcagtaaa
    17401 cttaaaggat atttacgcat attttaaaaa ctatctgtga tatatatcag ccaataaaac
    17461 atttacatta taaataaatt aatgattttc atttgaaatt cataataata aatagagact
    17521 atatattcac ataaaaaata taaattttaa aaaagggtgc aggagaccaa accatcacaa
    17581 ttcagtacgc ggggttttac ccccgcgtgg tggctacttc ttcgattcgt aagcgagaac
    17641 tgccttaaac tccataccac gttctctgat actcaactca cataacgagt cacctaccat
    17701 caacgtgaat cccagtactg tacaacacag tacgatgatt gccacaaggg catattttgt
    17761 cagcatgtct tgcctccctg caaagaagag actaacatcc gaattgctac gttcgaacgc
    17821 gagcctcagg ttgatagaaa tatcgcctgg ggcttttgtc catctggaac ctcgcgaatg
    17881 cttaacgcca gacagcctca agcacccaac gccattctat acctgttaat tttccctgcg
    17941 atgttcacct ttaaatgccg gaatgctgct cgcaaaataa aaacgccccc atcatttctg
    18001 acagaggcgt ttaatttatc cggcaatatt tacccttcgt gtaatccaca ttcccttttt
    18061 aagccaaaga aacgcgtttc ttcttccgcc atgccgggtt cccatttacg ggttgtatgg
    18121 gtatcgccca ccgataaata tccttcgtcc cataatgggt gatatttcag gccatgtttt
    18181 tgcaggtact gataaatagt tcggttatcc cagtcgataa tcggcagcac tttaaatacg
    18241 ccacgctgaa ttgccagcac cggtaaattg gcacggctgc cggattgttc acgccgcagg
    18301 ccagcaaacc acgtttgcgc attcagttct ttcagcgccc ggttcatcgg ttcgactttg
    18361 ttgatgtcat tgtacttttc aatgccttca acgccctgtt cccacagttt gccgtagcgt
    18421 gcttcctgcc aggcggcgct ttcggtagcg cggtacactt tcaggttgag cttgagtttg
    18481 tccgttaact cgtcaataaa gcggtaggtt tccgggaaca gatagccggt gtcggtgagg
    18541 atcaccggaa tatccgggtg aatctgattc accagatgca ggctcaccgc cgcctgaatg
    18601 ccaaagctgg aagaaagtac atattcaccg ggcagattat ccagcgccca ggcaacgcgg
    18661 ccttcagcgt ccagtttttc cagttcggcg ttagtttctg ccagcgccag aatgcgatct
    18721 accttcggca gttcgttcag ggcgtttaga tcgagtttgg acatagattc ctcactgttt
    18781 gccttgcctg atgcgacgct cacgcgtctt atcaggccta caagaccggg ctgatggtta
    18841 atcccacaaa tcgcgcgccg gatcgagcac cgggcgaatg atgcccgcac gcaccgtaaa
    18901 gtcgccgaag ccttcacccg cttcgcgctc tttcgcccag cgccctatca gttcatcaag
    18961 cgacgccagg atttccggct cggtgatgtt ttctttatac atccgtggga tacgtgtccc
    19021 aatgcggttg ccgccaagat gcaggttgta gcgacccggt gctttaccca ccaggcccac
    19081 ttccgccagc atcgcgcgac cacaaccgtt cgggcagcct gttacacgca tcacgatatg
    19141 ctcatcgctg acaccatgtt tcgccattaa attatcgatg ttgtcgataa aagacggcag
    19201 gaaacgctcc gcttccgcca tcgccagcgg gcaagtcggg aatgacacgc aggccatcga
    19261 gttttcacgc tgcggcgtga cggcattcat taacccgctc tctttggcaa ttttctcgat
    19321 cttcgctttc tcgctttccg gcacaccggc gatgatcaga ttctggttcg ccgtaatgcg
    19381 gaaatcacct ttgtggatct tcgcgatctc cagcaggcca gttttcagcg gacgccccgg
    19441 ataatcaagg atgcgaccat tttcgataaa cagcgtcagg tgccagttat catcaatgcc
    19501 cttaacccag ccaatacgat cgccgcgtcc tgtaaattca tacgggcgga tcggttcaaa
    19561 tttgatcccc gcgcgacgct ccacttccgc tttaaacgtc tcaaccccca cgcgctccag
    19621 tgtgtatttt gttttggcat ttttacgatc ggttcggtta ccccagtcac gctgagttgt
    19681 cacgacggct tccgccaccg ctagcgtatg ctctagcggc agatagccaa actcactcgc
    19741 cgtgcgggcg taggttttct tgttaccgtg ttcgatagaa agcccaccgc ccaccagcag
    19801 gttgaagccc accagcttgc cgttttccgc gatcgccacg aagttcatgt cgttggcgtg
    19861 cagatcaata tcgttctgcg gcgggatcac taccgtggtt ttgaacttac gcggcaggta
    19921 ggtctgacca aggatcggtt cttcatcggt ggtggcgact ttttcctgat cgagccagat
    19981 ctccgcatac gcgcgggtac gcggcagcag atgttcagaa atcttcttcg cccactcgta
    20041 cgcttctgcg tgcagctgcg actcgtaagg gttcgaggtg cagagtacgt tacggttcat
    20101 gtcgttagca gtcgccagcg catcaagacc gaccgagtgc agcatctggt gcaccggttt
    20161 gacgttcttt ttcagaatgc cgtggaactg aaacgtctgg cggttggtca ggcgaatgct
    20221 gccatagatg gtgttttcac cggcaaattt atcgatcgcc tgccactgtt tggtggtgat
    20281 aactccaccc ggcagacggc agcgcaacag catcgcgtgg cgcggctcca gcttctgttc
    20341 agcacgttcg gcgcggatgt cgcggtcatc ctgctgatac atgccgtgga agcggatcag
    20401 caggaagtta tcgcccttaa agccgccggt cagaccgtcg tttaaatctt ccgcaatggt
    20461 gccgcgcagg tagttgcttt caagcttcat gcgctcggcg tctgtcagtt ttccttcgac
    20521 cactaaaggc cctggatgtt tttcgctcat tagtagacat ctcgctgata acggcgctct
    20581 acgcgcagct cacttaaaaa ttcatccgcc gcttcggtgt ccatgccacc aaattcggca
    20641 atcacttcca gaagtgcctg ctcaacgtct ttcgccatgc gattagcgtc gccgcagaca
    20701 taaatgtggg caccgtcatt gatccagcgc cacagctccg cgccctgttc gcgcagttta
    20761 tcttgtacgt aaactttttc tttttggtcg cgcgaccagg cgagatcgat ccgcgtcagc
    20821 acaccctctt tgacgtagcg ctgccactcc acctgataca ggaagtcttc cgtaaaatgc
    20881 ggattgccaa agaacagcca gtttttacct ggcgcttcgt cagcggcgcg ttgctgcata
    20941 aaggcgcgga acggcgcaat accggtgcct gggccaatca taatcaccgg ggtttccagg
    21001 ttagcgggca ggcggaagtt atcgttatgt tcgataaata cgcggacttc gccctcttct
    21061 tccacgcggt cagcgaggaa gctggaggca ccaccggcac gggcgcggcc ttccacgtcg
    21121 taacgcacca caccaacggt gacgtgtact tcgttctcga cttccgcctg cgaggaggca
    21181 atggaataga gacgcggggt cagcgggcgc agcagattaa ttagcgcttc ggcatcaagc
    21241 tgtgccgggg agaaacgcac catgtcaaca atcagcgtcg tcgcggcgta atgctgtaac
    21301 ttcgctttat cgcccaccag cggcagcagt gtttcactgc gggtaagcgt ggcgtaattc
    21361 tcaacaatgt tggcggtgtt gacggtcagt tcgaagtgcc actgtagcgc ttcgttcaga
    21421 ggcaacgttt tgccctcgac agtgacaggt tcatcgcctg tcagccacag cagttcgaca
    21481 agttctttca ccagtgccgg atcgttctga taccagacgc ccagcgcgtc acccggctgg
    21541 taacgcaggc ccgagtcgcc taagtcaatt tcgatatggc gaacgtcttt ttcagagtta
    21601 cgcccagtaa ttttctggtt aacagagagg ctagccacca gcggcgcgtc tttgctgtac
    21661 gggctggtgt ggatttcatt taccgcgcca gtagcgacgg attgcgaagg tgccgcgaca
    21721 ggcgcacgcg atttaagcgc atcaaccacg cgggcgcgcc actcgctggc agcagcctgg
    21781 tattcaacat cagaatcgac acggtcgagc aggcgttcac cacccagttc cgccagcttg
    21841 ctgtcaaaat ctttcccgga ctggcagaaa aattcataag agctatcgcc gaggctaaac
    21901 acggcaaacg cggtgttttc cagctttggc gcttttttgg agaacaggaa cttatgcagc
    21961 gcgacggctt cttccggcgg ttccccttcc ccttgcgttg acgtcactac gatgagcagt
    22021 ttttcgctgg cgatttgttt gaatttatag tcgcccgcgt tcaccagctt aacgttcagt
    22081 tttgctgcta ataaatcatc gcgtaatgct tcagcaaccc ggcgcgcatt gccggtttgc
    22141 gaagcagaga taatcgttat acccggcatt tctgcggcgg gcgctggcgt cactgcaagc
    22201 gcaacaggct gctggttgag cacgccccag aaatagccag aaacccaggc aagctgggta
    22261 ggcgttaaat cggtcgtggc cgcctgaagg cgtgccagtt gctccgggtt caacggaagc
    22321 aacgcggaag gtgggacctg tgtcgtcatg cgtcgttatg ttccagtaag caaagctgtt
    22381 tctgcgccct gtcagcgccc ataaaacaga agagaaggta aggttaacgg ggtgaacggt
    22441 gtggattaaa gacgggatag cgataactaa taaccaaatc gactaacctg ttttagcaat
    22501 agtctttaac aacaaaatag attaaccaac ctaatgaaaa acaaatgaat ttagccaatc
    22561 attaagataa atcagcgatt ttgcgcaaca aagtcgtttt agataatgcg aaaaaacagc
    22621 ctttccggta ctctacggcg gttttatcgt cctgtagaga aattatgatg tccaccacgt
    22681 tatttaaaga tttcaccttc gaagccgctc accgcttacc acatgtcccg gaagggcata
    22741 aatgtggtcg cctgcacggg cattccttta tggtgcgact ggaaattacc ggggaagtcg
    22801 atccgcatac gggctggatt atcgatttcg ctgaactgaa agcggcgttt aagccaactt
    22861 atgagcgcct cgatcaccat tatctcaatg atattccagg tctggaaaac ccaaccagcg
    22921 aagttttagc aaaatggatt tgggatcagg ttaaacccgt tgtgccgctg ttaagtgcgg
    22981 tgatggtaaa agaaacctgc accgcagggt gtatctatcg cggcgaatga taagagtgtg
    23041 tcggcggtca atttcccttt agtaacgcta tgttagggtg ttgtgttctg gatatctggg
    23101 gcatgacatg gaagacgact gcgacattat tattattggt gccggtattg caggcaccgc
    23161 ttgcgcgtta cgctgcgcgc gagcggattt atccgttttg ttactcgaac gcgctgaaat
    23221 ccccggcagc aaaaatcttt ccggcaggcg gttatatacc catgcactcg cggaactcct
    23281 cccgcaattt catctgaccg cgcctcttga acgacgcatc actcacgaaa gcctttccct
    23341 gttaacgccg gatggcgcaa cgacgttttc cagcttacag cccggcggtg aatcctggag
    23401 tgtattacgt gcacggttcg atccgtggct ggttgccgaa gccgaaaaag aaggtgtcga
    23461 atgcatcccc ggagcgacgg tggatgcact gtatgaagaa aacggcagag tctgtggcgt
    23521 tatttgtggt gacgatattc tccgcgcccg ttatgtggtg ctggcagaag gtgccaacag
    23581 cgtcctggct gaacgtcacg ggttagtgac tcgtcctgct ggcgaagcga tggcgttggg
    23641 gatcaaagaa gtgctgtcgc tggaaacatc cgctattgaa gaacgttttc atctggagaa
    23701 taacgaaggc gcagcgttgc tgttcagcgg cggaatctgt gatgacttac ccggcggcgc
    23761 atttctttat actaatcaac aaacgctctc gttagggatt gtttgcccgc tctcttccct
    23821 tacgcaaagt cgtgttccgg caagcgagct gctgactcgc tttaaagcgc atccggcagt
    23881 gcgcacgctt atcaaaaaca cggaatcact ggagtatggt gcgcatctgg tgccagaagg
    23941 tggcttgcac agtatgccgg tgcaatacgc cggtaacggc tggctgctgg tgggcgatgc
    24001 gttgcgcagt tgcgtcaata ccggaatttc cgtgcgcggc atggatatgg cgctgactgg
    24061 cgcacaggcg gcggcacaaa cgctaataag cgcctgccag caccgcgagc cgcaaaatct
    24121 gtttgcgctt tatcatcaca acgtagagcg cagcctgcta tgggatgttc tacaacgtta
    24181 tcagcatgtt ccggcgcttt tgcaacgccc tggctggtat cgggcgtggc ctgcgttaat
    24241 gcaggatatt tcccgcgatt tatgggatca gggtgataaa cctgttccac cgctgcgcca
    24301 gttattctgg cgtcatttac gtcgtcatgg cctgtggcat ctggcgggcg atgttatcag
    24361 gagtctgcga tgtctgtagc ccgtaatctc tggcgcgttg ctgatgcgcc gcacattgtt
    24421 ccggctgact ccgttgagcg tcagatggta caacggttga ttaacgcctg tccggtaggt
    24481 cttttttctc tcacacagga aggtggctta cgtattgact atcgcggctg cctggagtgt
    24541 ggcacctgcc gtttgctgtg cgacgaatcc acactacaac agtggcgcta tccgccttcc
    24601 ggattcggca tcacctaccg ctttggataa ataataagga taatttatgc ccctcttaca
    24661 cctgctccgc cagaatccgg tgattgctgc cgttaaagat aatgccagcc tgcaactggc
    24721 catcgattct gaatgccagt ttatttccgt gttgtacggc aatatctgca ccatcagtaa
    24781 tatcgttaaa aagattaaga acgccgggaa atatgctttt attcatgttg atttgctgga
    24841 aggcgcgtca aataaagaag tggtgattca gtttttgaaa ctggtgaccg aggcggatgg
    24901 aattatcagc accaaagcat cgatgctaaa agcggcaaga gcggaagttt ttttctgtat
    24961 tcatcgcctg tttattgttg attcgatttc gtttcacaac attgataagc aagttgcgca
    25021 atcgaatccg gattgtattg agatcctgcc aggctgcatg cccaaagtgc tgggctgggt
    25081 gacggaaaaa atccgccaac cgctgattgc tggcgggctg gtatgcgatg aagaagatgc
    25141 gcgtaatgcg attaacgcgg gtgtcgtggc gctttccacc acgaataccg gagtctggac
    25201 gttagcgaaa aaattgcttt gacgggataa tcgtaaccaa ttgaatttgt tttgatttgt
    25261 tggccgcacg ccacatccga cattcagcgc ctgatgcgac gcttaacgcg tcttatcagg
    25321 cctacaggtc cccagtccag aaccgtaggc aggatcatcc ggcagcacgt tattggtatt
    25381 ctgcgtgaat gtttgtcacc agtgcctcca gcaccacttt ccagtcatca accacgtcaa
    25441 tatctgcctg tgaaaatacc gctgcgctgg cgtcatgatt aatcgccacc ataaacttgc
    25501 tattgcgtac gcccgccatt aacgctgctg cgccggaagc gccaacaaca atgcacacct
    25561 caggtgccag cagatgtccg gaaataccaa tcactttttc agcatcaacg ccaccgttca
    25621 tcactcgcgc ccgactgtag cccacttcgg ttcccagctt ttctgccagc ataacgattt
    25681 cctggttatc tgcctctccg ccctgcccca ccaccagcac ccgtctggcc tcagctaatg
    25741 ggtcgcggct aacatttttc agatcctctg tgctaaccag ccagtcggga agcgcacctg
    25801 ggacaatgtt cagttgctgc attccagagg gcaacgtggc gtttttcgct gctcccgcct
    25861 gacgcgccag ggaaaggcat aacgggcgtt tttctgtttg cagcgtcgcc gttaacgcat
    25921 ttccccagtg ggatttgcgc atgcttacgg ttggaatatc taacgaagtc acctgacaga
    25981 tgcttgcacc atgtaaacgc caggccagcc gggtcgacag ttcatcgcca aacgttcccg
    26041 gcggaaagag aacaacgtcc gcaggcgttc gctgccactg ttccaccatc gcatccagca
    26101 cctgttccgc cactacgggc tgaggttcta tttgccagtg cgccagcgta caaccgctga
    26161 aatcctgcgc cgccagccag ctcgctattg cggcattctc ctgatttatt gtcacaattg
    26221 cgatattcat ggctgcatcc tttggcgtaa atagtcctgc catagctttt gagctttttc
    26281 tgctaccgtt tgcccatcaa tcagcgtcgc tccgcatcgt tgctctgctc ttgccagttg
    26341 cagacactgc atcgctggca tttcggcagc aacagtttta cggataattt ctgctttccc
    26401 cgcagccatt cgctggcgca ttcccggtac tggcaaggcc acttcaccgc actgacgcac
    26461 ggcaatcacc gcaggcaagc gaacccggca acagcgcaac ccatgttcag tacgctgttc
    26521 gagggtgata aacagcgcgt cgagagtgaa acgctccacc tgggtgaagc agggccagcc
    26581 cagcatttcc gccagcaaaa atggcgtttg cccattctgc ccttcgctgc tttggcagcc
    26641 ggtaattatc agatccagcg gattctgacg ctgccattcg gcaatatggc gagcgacaaa
    26701 ttccggcgca aagcgcagat ctgccgccgt ctccagcaat acagcttcct caaacccaag
    26761 agccatgaga tagcgcagcc agtgcaacgc ccgttcatcc cccatgctca acgcggttaa
    26821 agacatcggc gtaccgtttt ttctctgcgc taacagcagc gcggcagcgg cctgttcatc
    26881 agcaccgagt aaacttcgca gtagcgaaac atccggtccg ctattaccct gagccgccgc
    26941 ctgccactct ttttccgcca gcattccggc atccggttcg gctttaaacg ctaacagaat
    27001 gttcattcgc acctcctaca tttcccgccg ccaccagcgg gagggcttta gtttctggtg
    27061 cccatagcca ggtcacaaca aaaccaacca acaacactgt cgccaggagc aataaggtga
    27121 cttgcattcc ccactgcgcc agcacccacg gcagcaggcc agtacttacc gccgcgccaa
    27181 gtcgactcat ggcggtggca aaaccgacgc ccagcgagcg aatgtcagta ggaaaacttt
    27241 ccgcaggcaa aatgcccacc agattactga ctgccgaaat ggtggtgctg aagagaacaa
    27301 aaagcagcag cgttaatgaa ctgccggaag gcaaacaggc cattactacc agcgttgccg
    27361 ccagcagcaa aaaacttccc agcaaaaatt tgcgatgtgc cagcaggtgc gtcagaacta
    27421 atcccagcag cgcgcccaca attaacaacg cattaagcat caggctggca gtcagcgcat
    27481 cttccagacc aatagtctgg gcgatagttg gcagccaggt ataaatcaca aaccatggga
    27541 ttacgaggca gacaaagaag acgctgttaa acgccgtgcg ccgccagtaa cgcgaagaga
    27601 acaaggtttt gatgtgttta tgggtcgccg ttaccacttc atcgcccagt aaaacatggg
    27661 gaccaaaata gcgatgcacg atagcgtgag cttctgcaaa acgcccctgg cacagtagcc
    27721 aacgtggcga ttctggcgtt ccccagcgta ataacgtaat caacaacgcg ggcagagctg
    27781 ccgatgccag caaccagcgc caggcctccg ggttttcgga aataaagtga tgtccggcaa
    27841 tacttgccaa cacatagcct acggtccaca ccacgctgaa tgcgcccagc aaaataccgc
    27901 gatggcggcg cggggaaaat tcagccagca aggtgtgacc tactgagtaa tcgcctccca
    27961 gaccaatgcc gatcaaaatg cgcagcccaa taagatgctc tggcgtggtg gcaaaaaatt
    28021 gcaagaacga agcgagcgta atcagcaaaa agctgaaggt gaagattttt tgccgaccaa
    28081 tatggtcgga gatccaccca agaaccaggc tgccaagaaa caaaccaagg agcgccgagc
    28141 cgccgatcat tcccgccata aacggcgtca gttgcatggc gggcgtaagt tgaataatgg
    28201 cgtaaccaat gacgccgaga acataaccgt cggtcaggtg tgcgccgaaa gtgagcgcag
    28261 caattcgaca gtgaaaacgg ttaagcggta aatcatccat tcgcaccggt gaagtgttca
    28321 tctttacccc ttcattgtcc ggtaataaga acggcttctc gcctgagaag ccgaaaaggc
    28381 aacgccccgt catcgcggaa cgctgcctga tacattattt ttcaatcgga tagatagtac
    28441 ccgtattcat aatgccgtta ggatcgaact gctttttcag cccttccagc aacgcccacg
    28501 cgctgccgtg ttccagcttg ctccagtgaa cgcgatgttt accaatgcca tggtggtgca
    28561 ccatcgaacc gccgaggcga atggtttctt cgcagatgat tttgttgagc ggattgtagt
    28621 acttgtcgat ttcctcttcc ggcttacagt caacaacgtt gtagtcatag acgaagtaca
    28681 tgttggtgcc gttctgatag ctatgagagg agtgaccgcc cagcatggtg atgtcgtcgg
    28741 cgtgcgggaa ctcagtacga atacggttaa taacgctttc gtagatttcg tggatgcagc
    28801 tccagcagcc ggacacttcg gtggtaaagc ccatgttgcc ggttttgagg atctgcacac
    28861 gttcggcagc cactttatcc ggtccccagt tcaggttgtt aaaccaggtt tcaatcagtt
    28921 tgctgtccac gcgctggcac tgcgggtagc gggcaacgat ttccgcaatc ccttcgcccg
    28981 tcgccttcgc aatgcgaggg ttaccttcag ccataaagat cagcacccat tttccgtcgg
    29041 caaaatgggt gaagtgttgg gtgccatctt cagcgtcata caaacgagcg atcgacggac
    29101 gatacccttc caccatgatt tcacgcagga tgttgaagcc ggttttcatg tcttccagga
    29161 tatagccgta gaagaggttg ttttccgggg tgtatttaaa gattttcact gttacttcag
    29221 tgatatagca caatgcacct tcgttgccga tgatgatgtg acgaatgtcc gggccagccg
    29281 cgcggcgtgg cacgttttta atgcgtgtga cggtgccatc tgccaacact gcctccagac
    29341 caacgaccat atcttcgatt gcgccgtaga gtgtggagaa ctgcccgata ctgcgggttg
    29401 ctaccaggcc gcccatctgc gccagcggct ttgactgcgg agaatgcccc gtggtgtaac
    29461 ctttttcacg caacgcgttt tccagcactt ccagcggaac accacattgc accgtcgcct
    29521 gcatattctc aatatcaata ttaatgattt gattcatggc ggagccgtcg agcaccaccg
    29581 agttttctac aacagtttcc agcccacctt cggtggcgga agcaccggta cgcggcacac
    29641 cgttaatttt gtgcgcattc ataaaattca gcacacggga tacttactct gtggaaccga
    29701 gttttacgac cgctgccgga atcggcaaag tataaatgcc atgaatatcc ggaaatttac
    29761 gaaaacggtc aatactgttt catcggtaat tacgcgatct gcaccaacaa tttcctttag
    29821 ctggtcgaca atcgctgcgc gagataaaga catagtaatt ccttcctgat aataaaaatg
    29881 gaataatcat taataagaga aaacagaaac caccgttatg gattagcgca ctaaataacc
    29941 gccatcgacc actaataaat gcccgttgac ataattcgat gcctgacttg cgagaaacac
    30001 ggctgcgccc attaaatcct gagtatcgcc ccaacggttt gccggaatat gatcaagaac
    30061 gcgctgattg gtttctggat tactgcgtgt cgccagcata atatgctgtt gcataatagc
    30121 caggggcgat accatttacc tgaatattat attgacctag ttcatcacaa taagctttgg
    30181 tgaacccggc aagagcatgt ttagtggcag aatatgcagg tgaccattgt ccacctaagt
    30241 aagagaacaa tgaacagata ttaataattt tgccgctttt ctgcgggatc ataatttttg
    30301 ccgcttcata gcttaactcg aatgcggcgg tcaggttcac atcaatcatc ggatcccagt
    30361 cggcacgacc gaagtccagc accttattca gcttacaaat accggcattg ttaaccagaa
    30421 tatcgacagt accgaagcgc tcacagcagg cagcgataat cttctgcggt gcgccttctg
    30481 cggtaatatc cacctgcatg aagtccactt caacaccctg tttttcaatc atttcctttg
    30541 tttcgccgtt atctttgacg aagctaggaa taaaaatatt tgcgccagct ttggccaacg
    30601 ccattgcaaa tgcctgacct aaaccgctat tcccgccggt aacaattgct gttttacctt
    30661 tcagggagaa aaaatccatt gagaacgcat tgagagattc gattgacata gttagctcca
    30721 atttccgtaa ggcaaaaaaa agaaaggtaa cttcccccgc gagagggaag ttacctttct
    30781 cattatctct ggtaactgga gataattttt agcataacgg cataactgct aatgtgatcg
    30841 taatcacagt gtgatattcg tattgtcaaa tagttttaaa aagcagaagc gtgattactc
    30901 tcacacaaca cctctgcttt ccatgttatt tcttattgca attactagag acctgagaaa
    30961 agtaatccac ctgaaagggc ggcttacttt tctctttttt ttgtctatta caaatgggaa
    31021 tacgatatgc aacacaactc atatcgccgt tggataaccc tcgcgataat tagttttagc
    31081 ggcggcgtta gttttgacct ggcttattta cgttatattt atcaaattcc catggcgaaa
    31141 tttatgggat tcagcaatac cgagataggt ttaataatga gtacctttgg tattgcggct
    31201 attattcttt atgcccccag cggcgttatt gccgataaat tttcacaccg caaaatgatt
    31261 acttccgcga tgatcattac cggattactg ggtctgttaa tggcaacgta tccaccgctg
    31321 tgggtaatgc tctgtattca ggtcgccttt gcgataacga cgattttaat gctgtggtcg
    31381 gtgtcgatta aagccgcctc gttgcttggc gatcatagcg agcaagggaa aattatgggc
    31441 tggatggaag ggctgcgcgg cgtcggtgta atgtcgctgg cggtgtttac catgtgggtc
    31501 ttttctcgct ttgcaccgga tgacagcgcc agcctgaaaa cagtcattat catctacagt
    31561 gtggtttaca tcttgttggg gattctgtgc tggttttttg ttagcgataa caacaacctg
    31621 cgcagtgcca ataacgaaga aaaacagtca ttccagctta gcgacatcct tgccgttttg
    31681 cgtatcagca ccacctggta ttgcagcatg gtgatttttg gtgtcttcac aatctacgcc
    31741 attctgagtt actccaccaa ctatctgacc gaaatgtacg gcatgtcgct ggtggcagcg
    31801 agctacatgg ggattgtgat caacaaaatc ttccgcgcgc tgtgcggccc acttggaggc
    31861 attatcacca cctacagtaa agtgaaatcc cctacccgcg tgattcaaat cctttccatc
    31921 atcggtttgc tggcgttgac agccctgctc gtcacgaact ctaacccgca atcggtcgcg
    31981 atggggattg gcctgatttt actgctggga ttcacctgtt acgcctcacg cgggctgtac
    32041 tgggcctgcc ccggcgaagc gagaacaccg tcttacatta tgggcaccac ggtaggtatt
    32101 tgttcggtga ttggattcct gccggatgtc ttcgtttacc cgattatcgg ccactggcaa
    32161 gacaccctgc ccgctgcaga agcctaccgc aatatgtggc tgatgggcat ggcggcgctt
    32221 gccatggtga tagtctttac ctttttgctg ttccaaaaaa ttcgtactgc tgatagcgcc
    32281 cccgcaatgg ctagcagcaa gtaagccagg aaaattacag gagaaaaatc atgtcgaaaa
    32341 aatacatcat agggattgat ggcggaagtc agagcacaaa agtggtgata tacgatctgg
    32401 aaggtaatgt ggtttgcgaa gggaaaggct tattgcagcc gatgcacacg ccagatgccg
    32461 atactgcaga acatcctgac gacgatttat gggcatcatt atgttttgcc ggtcacgatt
    32521 tgatgagtca gtttgccggg aataaagaag atattgtcgg tattggtctg ggatccatcc
    32581 gttgctgccg tgcgttattg aaagccgatg gcacgcccgc tgcgccgttg attagctggc
    32641 aggatgcacg cgtaacgcgt ccctacgaac acactaatcc tgacgtggca tatgtcacct
    32701 ctttttcggg ttatctgacg catcgcttaa ccggcgagtt taaagacaat atcgccaact
    32761 attttggtca gtggccggtg gattataaga gctgggcatg gagcgaagat gctgcggtaa
    32821 tggagaagtt taatatcccc cgtcatatac tgtttgatgt gcaaatgcca ggcactgttc
    32881 ttgggcatat cacaccacaa ggcgcgctgg cgacacattt cccagctggg ctgccggttg
    32941 tttgtaccac cagtgataaa ccggtagaag ctctgggggc cggattactg gatgatgaaa
    33001 cggcggtgat ttcactcggt acttatatcg cgctgatgat gaacggcaaa gccttgccga
    33061 aagatccggt ggcgtactgg ccgattatgt cttctattcc gcaaacattg ctgtatgaag
    33121 gttacggtat tcgcaaaggt atgtggacgg tgagctggct gcgcgacatg ttaggcgagt
    33181 cgttaattca ggatgccagg gcgcaggatc tttcaccgga agatttactc aacaagaaag
    33241 cctctagtgt gccacctggc tgtaatgggc tgatgacggt gctggattgg ctgaccaatc
    33301 cgtgggaacc gtacaaacgc gggattatga tcggctttga ttccagcatg gattacgcat
    33361 ggatatatcg ttcgatactg gaaagcgtgg cgctgacgct gaagaacaat tacgacaata
    33421 tgtgtaatga aatgaatcac tttgcgaagc atgtgatcat tactggcggc ggttcgaaca
    33481 gcgatctgtt tatgcagatt tttgccgacg tgttcaacct tccggcacga cgtaacgcca
    33541 ttaacggttg tgcaagcctg ggggcagcga ttaatacagc ggtaggtctg gggctatacc
    33601 cggattacgc aacggctgtc gacaaaatgg ttcgcgtgaa agatatcttt atgcctgttg
    33661 agagcaatgc caaacgctac gacgcgatga ataaaggtat tttcaaagac ctaaccaaac
    33721 atactgatgt gatcctgaaa aaatcgtatg aagtgatgca tggggaattg gggaatgcgg
    33781 attcgatcca gagctggtcg aatgcgtaag tgaggaagac cgggcgggaa actgcccggc
    33841 ctgaatatac ctgaatggtt atccccgctg acgcggggaa ctcgaataac tgaccaacgt
    33901 ctggctctct aaatccggtt tatccccgct ggcgcgggga acactctaaa cataacctat
    33961 tattaattaa tgatttttta agccagtcac aatctaccaa ctttacagga tcacacaaac
    34021 aacacatcca ttatgttaaa gagcacttaa tccattgatt aaaaaggtaa atatttaaaa
    34081 taactctata caaactaaaa tctaccaaac ttcgccgcaa taattttcac tccagcaaaa
    34141 aattaacgcc gcagaatttg tagagaatga taatgattag tccgggatgg gcaagatgga
    34201 tgagttttca ggggcgacaa ataatacaaa tgagtggaat tttttacaag ttccacccat
    34261 ttataa
//
LOCUS       DADMEQ010000031        33498 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.031, whole genome shotgun sequence.
ACCESSION   DADMEQ010000031 DADMEQ010000000
VERSION     DADMEQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.031"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            1319..2098
                     /gene="ipaJ"
                     /locus_tag="JNZ05_001544"
     CDS             1319..2098
                     /gene="ipaJ"
                     /locus_tag="JNZ05_001544"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HAY8564818.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3046..3975)
                     /locus_tag="JNZ05_001545"
     CDS             complement(3046..3975)
                     /locus_tag="JNZ05_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HAY8564819.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4192..4428)
                     /locus_tag="JNZ05_001546"
     CDS             complement(4192..4428)
                     /locus_tag="JNZ05_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY8564820.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4432..6333)
                     /gene="ipaA"
                     /locus_tag="JNZ05_001547"
     CDS             complement(4432..6333)
                     /gene="ipaA"
                     /locus_tag="JNZ05_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HAY8564821.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLIDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRTNNKLKVTDANKINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSQIDDTSAELLTDDISDLKNNNDITAENNNIYNAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6342..7340)
                     /gene="ipaD"
                     /locus_tag="JNZ05_001548"
     CDS             complement(6342..7340)
                     /gene="ipaD"
                     /locus_tag="JNZ05_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HAY8564822.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNKALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRK
                     GYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7391..8482)
                     /gene="ipaC"
                     /locus_tag="JNZ05_001549"
     CDS             complement(7391..8482)
                     /gene="ipaC"
                     /locus_tag="JNZ05_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HAY8564823.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSAQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSI
                     SSNAVSLIISVAVLLSALRTAENKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSITQSRNSAASQIAGNIRA"
     gene            complement(8502..10244)
                     /gene="ipaB"
                     /locus_tag="JNZ05_001550"
     CDS             complement(8502..10244)
                     /gene="ipaB"
                     /locus_tag="JNZ05_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HAY8564824.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINR
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10250..10717)
                     /gene="ipgC"
                     /locus_tag="JNZ05_001551"
     CDS             complement(10250..10717)
                     /gene="ipgC"
                     /locus_tag="JNZ05_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocator chaperone
                     IpgC"
                     /protein_id="HAY8564825.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10774..11400)
                     /gene="ipgB"
                     /locus_tag="JNZ05_001552"
     CDS             complement(10774..11400)
                     /gene="ipgB"
                     /locus_tag="JNZ05_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgB"
                     /protein_id="HAY8564826.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSHNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11416..11805)
                     /gene="ipgA"
                     /locus_tag="JNZ05_001553"
     CDS             complement(11416..11805)
                     /gene="ipgA"
                     /locus_tag="JNZ05_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HAY8564827.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11818..13302)
                     /gene="icsB"
                     /locus_tag="JNZ05_001554"
     CDS             complement(11818..13302)
                     /gene="icsB"
                     /locus_tag="JNZ05_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HAY8564828.1"
                     /translation="MSLKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGDVTKDTEINYLFEKRLGYSVDTYKQDKLNMISDQTAERLDAGQEVINLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDVVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13616..15232
                     /locus_tag="JNZ05_001555"
     CDS             13616..15232
                     /locus_tag="JNZ05_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HAY8564829.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVYYEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESEHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGEPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIINKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15242..15604
                     /gene="ipgE"
                     /locus_tag="JNZ05_001556"
     CDS             15242..15604
                     /gene="ipgE"
                     /locus_tag="JNZ05_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HAY8564830.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15604..16062
                     /gene="ipgF"
                     /locus_tag="JNZ05_001557"
     CDS             15604..16062
                     /gene="ipgF"
                     /locus_tag="JNZ05_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgF"
                     /protein_id="HAY8564831.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16071..17177
                     /gene="mxiG"
                     /locus_tag="JNZ05_001558"
     CDS             16071..17177
                     /gene="mxiG"
                     /locus_tag="JNZ05_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HAY8564832.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKKIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFFR"
     gene            17195..17446
                     /gene="mxiH"
                     /locus_tag="JNZ05_001559"
     CDS             17195..17446
                     /gene="mxiH"
                     /locus_tag="JNZ05_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex protein
                     MxiH"
                     /protein_id="HAY8564833.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLARNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17459..17752
                     /gene="mxiI"
                     /locus_tag="JNZ05_001560"
     CDS             17459..17752
                     /gene="mxiI"
                     /locus_tag="JNZ05_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HAY8564834.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17758..18483
                     /locus_tag="JNZ05_001561"
     CDS             17758..18483
                     /locus_tag="JNZ05_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HAY8564835.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18480..19007
                     /locus_tag="JNZ05_001562"
     CDS             18480..19007
                     /locus_tag="JNZ05_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HAY8564836.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18949..19644
                     /locus_tag="JNZ05_001563"
     CDS             18949..19644
                     /locus_tag="JNZ05_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MxiN"
                     /protein_id="HAY8564837.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALSKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19637..20044
                     /locus_tag="JNZ05_001564"
     CDS             19637..20044
                     /locus_tag="JNZ05_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa secretion machinery protein MxiL"
                     /protein_id="HAY8564838.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            20028..20456
                     /gene="mxiM"
                     /locus_tag="JNZ05_001565"
     CDS             20028..20456
                     /gene="mxiM"
                     /locus_tag="JNZ05_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HAY8564839.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20586..21218
                     /gene="mxiE"
                     /locus_tag="JNZ05_001566"
     CDS             20586..21218
                     /gene="mxiE"
                     /locus_tag="JNZ05_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional activator MxiE"
                     /protein_id="HAY8564840.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21205..22905
                     /gene="mxiD"
                     /locus_tag="JNZ05_001567"
     CDS             21205..22905
                     /gene="mxiD"
                     /locus_tag="JNZ05_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HAY8564841.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22924..23991
                     /gene="mxiC"
                     /locus_tag="JNZ05_001568"
     CDS             22924..23991
                     /gene="mxiC"
                     /locus_tag="JNZ05_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HAY8564842.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSQASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24004..26064
                     /gene="mxiA"
                     /locus_tag="JNZ05_001569"
                     /gene_synonym="virH"
     CDS             24004..26064
                     /gene="mxiA"
                     /locus_tag="JNZ05_001569"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiA"
                     /protein_id="HAY8564843.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26077..26478
                     /gene="spaK"
                     /locus_tag="JNZ05_001570"
     CDS             26077..26478
                     /gene="spaK"
                     /locus_tag="JNZ05_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone SpaK"
                     /protein_id="HAY8564844.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26482..27774
                     /locus_tag="JNZ05_001571"
     CDS             26482..27774
                     /locus_tag="JNZ05_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HAY8564845.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27867..28205
                     /gene="spaM"
                     /locus_tag="JNZ05_001572"
     CDS             27867..28205
                     /gene="spaM"
                     /locus_tag="JNZ05_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface presentation of antigens protein SpaM"
                     /protein_id="HAY8564846.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28192..29070
                     /gene="spa32"
                     /locus_tag="JNZ05_001573"
     CDS             28192..29070
                     /gene="spa32"
                     /locus_tag="JNZ05_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HAY8564847.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29064..29945
                     /gene="spaO"
                     /locus_tag="JNZ05_001574"
     CDS             29064..29945
                     /gene="spaO"
                     /locus_tag="JNZ05_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SpaO"
                     /protein_id="HAY8564848.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29950..30600
                     /gene="spaP"
                     /locus_tag="JNZ05_001575"
     CDS             29950..30600
                     /gene="spaP"
                     /locus_tag="JNZ05_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaP"
                     /protein_id="HAY8564849.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30615..30875
                     /gene="spaQ"
                     /locus_tag="JNZ05_001576"
     CDS             30615..30875
                     /gene="spaQ"
                     /locus_tag="JNZ05_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HAY8564850.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30875..31645
                     /gene="spaR"
                     /locus_tag="JNZ05_001577"
     CDS             30875..31645
                     /gene="spaR"
                     /locus_tag="JNZ05_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaR"
                     /protein_id="HAY8564851.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31652..32680
                     /gene="spaS"
                     /locus_tag="JNZ05_001578"
     CDS             31652..32680
                     /gene="spaS"
                     /locus_tag="JNZ05_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein SpaS"
                     /protein_id="HAY8564852.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32693..32935
                     /locus_tag="JNZ05_001579"
     CDS             32693..32935
                     /locus_tag="JNZ05_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564853.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10457 a         4859 c         6709 g        11473 t
ORIGIN      
        1 tggatctgat ttcaggcgtt gggtgtggat cactattgca ccgttcgtga cacttgttga
       61 ctacctgtgc ggcatctggt atcaaggtgg cgtgaagccg gttcaggttc tcatgcggaa
      121 cgttggaacc tatatccccc tgataccaaa ggcgaaatgt aagtgacaaa atgcaatgaa
      181 cacgttctcc gttaactgag gagcatggtg ataaataaag gtaaatgcag aaggtgtaga
      241 gtctcgcaga tttctgacat catagagcct aacattagat gcataacgct acagattgta
      301 gaatctctac aggtgaaata tacactggtt ggcgtttatg tcgcaaaacc tgtcattcag
      361 gttcatctca ccaataagga tatgagtgaa gtggaggata agtgagtctg ctgtcagagt
      421 tttctggtgt atgtcagtaa ctgcgaacca tgtctgcttg atatgggagc ttatggaggc
      481 tctctgtaca aggttaggga gctaacaaag cagggataaa aagtccgcct gatgcaggca
      541 cggttcgtta aatcttttgc cattggtaac aaaatggtgc gatgaatgtt caggctatct
      601 ttatcttgat ggtggttcag ctgtttggta aagaaatcgc tgtaacaata gaagaactgc
      661 aggcagtact ggtcccacat cgtttgctca gtcaactggt gaagttccgt agcgcgtaaa
      721 ttaatgcttt gcatgaaacg ttactggagt ggatgaaaga atacctgcat atatcattac
      781 tgcgttggat taccagtaca acagattgaa tgaactggac atgttgatag acggcattga
      841 aaagcagctt ataagcatag tgcggcagag ttaattggag agcacttcgc cttctgagta
      901 ttaagtaagc atggtggtac ctgcggatac tatctattca ctgtgctcgg tcggcagcct
      961 tactgaccaa ggagttgggg gagttgataa caaagttgaa aaaacagctc tcaaccaagg
     1021 tggttattgt aacaatagca aataagctgg tgtgacctgt atcacaataa ccgattatgg
     1081 acgtaaatac gacaaaaata tgtcagtatc aggcggtatt aattgctgat gctgatggac
     1141 attaactctt aataaaaggg tggatgctga aaggttgtta tggtgacgga cgtgatgacg
     1201 acggaaaaga gtatgaggtt ttggatttaa agttcgttcg gggaataagg acccggtcag
     1261 ctaattctca tagtagttcg cattgctgga tgcaatactg tgctgcaatt actcgtcgat
     1321 gtcggaacaa cggaagcctt gcaaacgggg atgtattcat acaggtgtta tgttatatgg
     1381 ggttttattg cagggggcta taccaagaga atatatgata tctcatcaga ctgatgtgag
     1441 ggtaaatgag aatagagtaa acgaacaagg ttgttttttg gcgaggaagc agatgtatga
     1501 caattcttgt ggagctgcca gtttactctg tgctgcaaag gaattagggg ttgataaaat
     1561 accacaatac aaagggtcta tgtccgaaat gacaaggaag agttctcttg atttggataa
     1621 tcgttgtgag cgggatttat acttaataac tagtggtaat tataatccga gaatacacaa
     1681 agataatatc gcggatgccg gttattctat gcctgataag atagtcatgg ctacgaggct
     1741 tcttggattg aatgcttatg tcgttgaaga aagtaacatt ttttcgcaag taattagttt
     1801 tatttatccc gatgcaaggg acttattaat tggcatggga tgtaatatag tacatcaacg
     1861 agatgtgtta tcttctaacc aaagagtttt ggaagctgtt gctgtatcct ttattggagt
     1921 tcctgtcggc ctccactggg tgttgtgtag acctgatgga agctatatgg atccagctgt
     1981 aggagaaaat tattcttgtt tttcaactat ggaattaggt gcaagaagaa gtaattctaa
     2041 ctttatagga tatactaaaa taggtatttc catagttata actaatgagg ctttgtaaat
     2101 tgtcgttttt tatctgtgaa ggagcttgaa tattttgggg cggggggggc ataacaaatg
     2161 atattactga gtacaaattt agctatattg ggcggattag ttgtgtggga agacgaaatg
     2221 tttctcttga aataagtgaa ctccctcaag gaatgattgt gtgcttaaat atttaagtgc
     2281 tggtaagttt gagaaggctg acatattttg cagagcaaac agtgattgac ttggatgata
     2341 tttttatttt agtcactcgt tgtaattgga gtatgcttta ttttatttga aaatagatgt
     2401 attttatacc cgcaatttaa actgtaagcc ctatgagggg tatggatatt ataagttact
     2461 ctttgactaa gcataacttt tggggttaga aaaataaatc tcttgttaat aattaatatg
     2521 attttaaaat caaacttgtt tataattata aaataatgta agtcggtcat tttatgggaa
     2581 gattaggaga ataaaacagt aattatattt gagaaatctg ctaatatatt cttaaagctt
     2641 ttttgatact gtgaacgaag tctgttaatg gtggattttt tgtttatcac catgtaaaaa
     2701 aattgagtag taaaagcgtt gactgctaat cctcgctgtt tttattgaaa cgattaaaaa
     2761 tacgctgaat attttcggcc agttactcat cttcacataa aattttgacc atcatgccgc
     2821 atcccttagt tgcacaattc attttcaatg atatgaatat tactaatttc accagataat
     2881 tttctcgtgg tcagcagggt atgagttttc accgtaattt taaggaaaca cagtgcgtgg
     2941 ggattgaata acagatagcc gacggaatta ccccaccggc tgaaagtcaa gttcaacgaa
     3001 gatttttatt atctgaattg ggcagtttac atcagtgttc gatgtttatg aagacgatag
     3061 atggcgagaa attatatccc gaatagcttc atctatttct ctctgaactg ttttgtttat
     3121 tttagataat gtaaatatta gagttctgtt tgttttattc tcttttcttt ttatgtaagt
     3181 ccgtttgtct ttagaaataa ataacgagtc aacttttagc gaagggtttt gctttctgtt
     3241 tttgcttttc tttatgatgt ttaaaatttc ttttttatat atgtccgggg gcaaatttgt
     3301 atcaaggtct tttatttctt cctttaatat tggtagtgta gaactaagag attcattagc
     3361 cttttcaagt cctttataat aattgaataa tatcttgtag tcattaaagt taagttctga
     3421 cgcgattgga aatagagata ttatttcctg tggaacgctt gctgcctgaa aggcacgagt
     3481 gactttcgcg cgagacagat tctctttttt ggctatgtct ttataggaca tccctgatac
     3541 tttcagaaaa ttaagaccaa taccaagttc tcggatgcta tgctcttttg ctgtttgtat
     3601 atcgtttgct agttttctgg catcaagagt agatatatat tcttttgaat atagaacttc
     3661 aagatctgct cctgcatata ttgcagatgc tcttctacga gtgccatcca gaatttcaat
     3721 tctaccatca atctcccttc ctattacagg gaagaattgt tgtagcttta tagtttttat
     3781 gatatcggct aatgattctt ctgttaggga ttcttgatcg cgtacattcg ttttttggtt
     3841 gacgaaggtt aaatcttgga tccttttatg aggaatcttg gctttgataa aggaaacttt
     3901 attaccagaa tggagtgtaa actctttctt ttggccttcc tttatactta acaagtcgtt
     3961 gcacaaatcc accatatcac accctgttta ttcatattga tttatattga caaaagttaa
     4021 atgcagtgtg agcaagattg tatgtaactg ttgatgatag tcaacggtag tcttaaaaat
     4081 tactatttta atagctggat tgatgcaatt tatctttatt ggataaaaga ataatattct
     4141 tgtggagctc tgatgtgaga gctccataga tgaaaaaatg cttatcaatt actatattga
     4201 ttttgtacta tttttgacta cagaaataag atctccaata gtaataatgt gttctaatgc
     4261 agattcatct atacgtagat tgaactcaaa actaatcata tccagcattt caatcagaga
     4321 aagagaatcg gcagaaagat cttcaataaa tttagtttct tcagatagtt tgctgtacgc
     4381 tataccatag caaaaggcca ctattgataa tattttttct tttatcatat tttaatcctt
     4441 attgatattc tttaatactt ttgataggga agtggttaca tcttttgctg cgttatatat
     4501 attattgttt tcagctgtaa tatcattgtt attttttaaa tcagatatat catctgtaag
     4561 taattctgca gaggtatcgt caatttgtga caataccttg ctgagggcgc tagatacttc
     4621 ttttgccttt tcatagatcg catgattttt atcaattgta tctgcgttgg tttctgttac
     4681 gtagtttatt ttatttgcat ctgtaacttt taatttgttg tttgttctgt gttctgttgt
     4741 ggattttgta atgtcctttg atttttcctg taactctcgt tcttgtgttc ctgatttagt
     4801 tccaatgaga gatataagat tacttaggga gtttgttact ttttttgaag cttcaaatat
     4861 cgtttcccgt gttaaatatg tgtctccatt tcttgataaa aaatcagtct gaagtacttt
     4921 tgaatcagtg tttgagttga atttatttat ttgcgaagct actctatgag aaagagaagc
     4981 gctactttta gttgtatctg atgagttatc attactgctt gtcactcctt ccgggtggag
     5041 agggggggtg gttgaagagt tctgtatgtt cagaggcctg tgtccccgag aaagagagtt
     5101 atttgcagat ctttcttgac tactcgtagt taacaggtct gaacctattc catccgaatt
     5161 atctggagta tggttgaaaa ccttttctaa agctgaatta tttgcaaatg tctggacagt
     5221 atctgatata gagtgtggta gaacggaagt tactttttga actagtgctc tttgtggatt
     5281 tagtaatgag tttccgttac gggataatag atcgtttgtc tgagaagtag gagactgtgc
     5341 atcattttct gggttctcgt gatagctatt gtcatatact ctgttgtcaa aaactctatt
     5401 atcgtaagtt ctattatcat tatttatatg atagtgaatt acaggttggg atgttttctc
     5461 attggctccg ggggttggtc cgccatctgg catatcttcg ggaggtgtag ggaggccatc
     5521 ttggatagga tgcgaatctt ttatatcttc taaaatatca ttaaggactt ttctcaacag
     5581 ctcaggaaac acggctactg tatttaagtc tcttgacagt ttacctatac caaatccttt
     5641 actatcaata gtatttttac gttgattgcg caaactatca aaggcaagtt ttgcctgtgc
     5701 gtcaattaaa tcatctagtt tgagagatga aacagtttca ttacttaact ttaattcagc
     5761 aagaatatta ttcttgataa atgatgatac ttcagtattg aggagttttt gtcccaatga
     5821 taaagcttca ccagatgtgt gttgttgtgc taatagtgca agtctgtgct caatgttttg
     5881 tcgtaattga tggagtttct tattctttgc cgaccatggt ccaaaggcag aatcttggga
     5941 taatttatct tttataatta gttccgaata ctgtgcgaca attttttgaa gtcccttttc
     6001 gttttcaaaa ttaagaccat tttgatgtac taaggagtac aaaacatttg aaaattcgag
     6061 aatctcgctt ttggcatacg cattcattga gttggaataa atgcttctta acacatcttc
     6121 tttccctgag gaagaaaata aaaaatcaag acatttctga ttaaaagacg ttgcaaagtt
     6181 ttctttttca gacactgatg ctggtgtttt tagagaagtt tgcgaactat ggatatcgga
     6241 tattttgctt tttaactcgc tgtattctgt tgatgatggt gaagttgcct tatataagaa
     6301 tgttggcgct tgagtattat ttacattatg catggcgcac ctcagaaatg gagaaaaagt
     6361 ttatctgtat ctgtacatga gcttattgta ctactcaaaa cctttactaa attatcaaaa
     6421 atactattgg cattactgta tttttgaact aaagtttgaa gattattttt cattgtttca
     6481 tcttcggcag agaatccggc attccatgcc tgatattttg cattatctag cacaacctcg
     6541 ccatttccac ctagattatc taagcttttt aacatattgt ctattggggt catgtttata
     6601 ctgacaacat atcccccgtt tttttgagat accttgccga ttgttccacc taattctgta
     6661 agccatttat ttgcttgttc ctgactaaca gtattatttg ctggatatag cggtttatct
     6721 ttatattttt ccttgagttc ttccaatgcc tttttaagcg agttgacttg taatttcacg
     6781 gagtttccgt cgttacctcc gggagagatc cagccggcaa gactggaaag aacagcgcta
     6841 aaatcttgat acatttgagt atatgaacta acggcatgtt catatacttt cagatactgt
     6901 tcattaatat cattgatgga gtttgcaatt ttagcccaca gttctctatg agatatcatt
     6961 tgatctccat caagctcggc ttctttcggg gctgaatgta ataattctct tgcgtcttta
     7021 ttaattggat atcctttcct ggaaagaata tccaatagtt gagcggattt atttacatcc
     7081 atgctaatct gagatgaatg taatgctatt tcttctagcg atgttttagt caacgttttt
     7141 tgtgaaagct ctttctttaa tgccttattt gttgttctga tattatgaag ggtgtcgtta
     7201 agcatagtaa gggaacttac aggatgagaa ctggtcgttg tttttatatc agaattaact
     7261 gtttcggttg atgaaccgtt ggtattgttt ggactgaatg atgaggtgga aatactatta
     7321 gtcagagttg ttatattcat ggttatttcc ttacaggaag agccatatat tattatggct
     7381 cttggatata ttaagctcga atgttaccag caatctgact ggctgccgaa ttccttgatt
     7441 gggtgatgct gtcaattata ttcaataatt tttgtattaa ttgatttgtc gcttgggatg
     7501 cttctttaga tacttgggat gcttcctctg cttgtttact gctggcctga ctgattagtt
     7561 gttcttcttc ttcaagagca gatgcataac gccctcctga tgtggatata ttaccagcaa
     7621 cggctgatgt ttccattgtt gcacggccaa tgttttgttt ttgctgcgca gaaagggtat
     7681 tgagctcgta agttcttctc tgggtgtcaa ttttatcctg caaagaaata tcgggagaac
     7741 taagagaagt tttatgttca gttgacaggg atatattatc tggcgccagt ttatttttac
     7801 ctaaaaattt tgtgctagag ttagtttgtg gtgaggtgat atttgtatct atttgtttat
     7861 ttaaccctaa tttagaacct gcaagctctt tttcaagaga ttgagcagtg gcaaggttct
     7921 ttcttaaggc tcctttttgg tcgctaatcc ctgaatgcgt ttttttggca ccgatacccg
     7981 ttatacctac ttgtgtgact gctccagtaa tgcttgatga tagggctgcc aggccttgcc
     8041 gaacaatgtt ctctgcagct gattttgtag catcgaacgc aatcaatgac aattgagagc
     8101 ccaatttatt ttctgcagtg cggagagcag aaagtagaac ggctacacta ataattaaag
     8161 aaacagcatt agaagaaata ctggaaatat ccagagtgtt ctctggagta agggtgtgac
     8221 tcatctctga aagatctttc atctttttgt cagtaaggcg agcaatgatt tctgaatcat
     8281 gctgaacctg cgctgataac tttaaaagtt gatcatcatt taatgtggtt gttaatacgg
     8341 gatttttacc gacattaagt ggaatatgcg ctgcaatctg ctgataattt tgtgattttt
     8401 gtgtttcgga agaactttga gtttgttttg tggatatatc tgtatataaa atctgggttg
     8461 gttttgtgtt ttgaatttcc ataacattct ccttatttgt atcaagcagt agtttgttgc
     8521 aaaattgctt ttgcaacatc agttctatta gcctgagagt tggacattga ggctaataga
     8581 tctgcaatta cttcctgcaa ttggccgaat ttttctattg cttcactgat atattttgac
     8641 agttgttcaa cttgatattt cgataatgtc aggtctgcta gatttgtcga cgcgctgttc
     8701 tggaaaacag cagaagcgac acttcctccc gcttgtgtgg cagagttaac actttctcct
     8761 aataaaactg cttggtttaa atgggtggaa ataatttgtt tggatattac ttcatcaccc
     8821 gctgcaccaa gaaatttatt taatctggca acagcattag tgattaaatc gtccagttga
     8881 gaagaaaaat tcttgagaaa ctttggtata aggtctgtga gggttttacc tattattttg
     8941 ccaatattct ctgcaagttt tgctgctgcc tgtttaccaa cagtggctac gagaacgact
     9001 gctgcaacta ggacaagagc gcctgcgatt gcccccagaa tagagccaat cattttggct
     9061 tttttcgagt cgacgcccaa gccttcgagc atttttgtaa atgcatctga aaggagtttg
     9121 attaagggtt caatgactgc tttcatgatc ggattcaggg cttgttccat gaaggaattg
     9181 ccggtcgctg cttgtactat agcatccgta accataagag ctaaaccaac agctgccagt
     9241 gctagagagg ctcctccaga aaaagctgct gcaacaacac taacgatagt taaaagtgcc
     9301 ccaagtattt tcccaacaca acccattact ctgttgagtt cttctgcttt acgtacttca
     9361 gcagcatact catcagattt tctctccatt tcagtttttc ttgattcttg gagagactgg
     9421 aatagagcca gatcattttt taaagattct tcattatttt ttccaactag ttgaataaag
     9481 gttgccatca attgagtaac actggcaagt ccggttaatg atttctgctg ggttgatagc
     9541 tgttcagcag atgctgtgtt tgaaaatgca gaaaaagagt ctatttcttt ttcgagttgc
     9601 attgatttat ctgtaagttt gctatgaatt gacagggttt tctgctcaat caatgtcctg
     9661 tctttaactg ctgcgtcttt tttgatagtg agttgtattt cttcccggct taattttttc
     9721 ttttctggtg actctgggtc gagttcggat aatcttgttt gaattcggtt aattttattt
     9781 tctaggtctt ttattttaga atctgcgttt tttagtttat taatttgttt ttcatagtct
     9841 ctggtaagtc cttcagtttc agatagaaga gtgttaattt tatcggagaa ttctaggttt
     9901 ttttgctgtc ttgcctgttg ctgggacttc caagcagtaa ttttatttgt taatgcagtt
     9961 aaagattttt caccgagtat ttgaataagg tttccaatta aaagcgttag ttgggaactt
    10021 gcatttaatg actttggtgc tttaagttca gggattaata tatttgaagt tgagtgtgca
    10081 ttagttgtat taatattttg gttagcagta agatcagcaa ttgtaagaga aaataatttg
    10141 ttagctgcat catttgcagc ttggatagta ttgtctccaa gctcagtgga agccaatatt
    10201 ttggcaagag gaaaaccagt ggttgtggtg cttacattat gcataataat tactccttga
    10261 tatcctgaat tgcgtccaag tatgattgtg cttttatttt taatttttca tcattgctgt
    10321 gttgaattac gagttcgaag cactctttag cttttaaggg ggcttttaac cgaagctggc
    10381 attgtccagt atggaatact ggtgtatagt cattttttcc taatgcaaaa gcgacagcat
    10441 aaaggtctgc tgcttgttgg aactgttctt ttatctgata aatagctgcg agtcccataa
    10501 tgtagtctac attgtaaaag tcgtatatac ataaaaatct gaagaaaact tcagcttcct
    10561 ctattcttcc tttgttgtaa aagtcataag catatgaata aatgtcatcc atcatatcat
    10621 caggaattgc attaatatct ttcagtgtag cgccagagtt aattgcatca attactgcag
    10681 tagagatgct ttcattttca gtgatattta aagacataag gtctcctttt tgtctgcttt
    10741 actttaataa gtataagatt taatataaaa gatttaattt gtattgcttt gacggtatac
    10801 agcctttaga acctctgcgc ttatttctat ttgtaaaact tttgttgatt cattaaatat
    10861 tttatcgtag acggaactgg atactagatt tgtaagatcc ttcatttgtg tgttaaacaa
    10921 atccatgtca ctgctatagc gttttaaaat ctttgctctg aggccagatg aggcaatctc
    10981 gtttagtgct gtatgactga tagatgtttg tgctgatttt tcatcaagtt ttatatccgg
    11041 gaattttttt tcaatagctg caaatatgtt ttttttgtta tctgaggtca aaacaactcc
    11101 attgtgtatg gccatttcat ctatacttct gtttatcatt gaagagacat aagaggttcg
    11161 ctcttggctc atccaacaaa atagttgatt ccccgaatta ctatctttta ttttgtctat
    11221 gacgctgtcc ctgattttat ttatattcac cgggcatcca aaattcttct gaggaaagcg
    11281 ttgttttaag ttaaaaacag ataatatttt ctttacaagc ttattgtgac ttaacgaatc
    11341 aaatgatggg cgaggaatcg cgaattcaac ctgtggaagt attttgttta gaatttgcat
    11401 gataccccct atatgttagt tcacttctga agtgatgttt gcaatggtta gtttaataca
    11461 aaataaaaat tcatacaaag attcaatgag ttcatcaggt gaaagatatt ttttgtagtg
    11521 tttcttggtc aaaacacatt gatgagtatc agggagaaga cagatagaag aaccataatc
    11581 tctactcaaa gagtagttaa ggatgagtat gtgttctgga ttaataatat tttctgtaaa
    11641 aagttcatgt tcattaagaa gtccggtcaa aaagatgtat tcatcattat ctgttataca
    11701 aacaggtatt tgttcttcga gcaatataaa ggcttgattt ttatcgttaa aatcaagctt
    11761 tgcacctgtt atttcataaa gtttatcata tagtttgcga cacatttcta tggccttcta
    11821 tatattagaa tgagagttat tcaataaaag cttattaatc tgtaataagt cgtgaatgta
    11881 aagtttggtt ccaagatctg gcgatttaag agaattgtaa taatcaataa tttttattga
    11941 tttttcgata atttcttctt ttttatctgt tgcttcggtt tttaattcgt ttaatacacg
    12001 agtcatattg ctgggtgctg attttactaa gtatgagtgg caagcgttga tgaccacatc
    12061 aaagtcttct gaagatacat tttcagtagg atgtttgtaa agtgaaattg ataaaggctt
    12121 gaatgttttt ggtgtgtgga taaggttaat tttattgaaa cttttaagat agctgcgtcg
    12181 taattgagtc aatctggaaa gatcaaaatc agattcaata tacttcatta tttcgttata
    12241 agactgattg agactttcaa tgcgttgcct aactttattt gcatacgcgt atacatcgtt
    12301 aggtgtagca accaatttaa cgtcagggag tggaaagtat atttcagaat ttcctgcttt
    12361 aagaacatta agtgccatac cagcacagtt ttcagattta ctgattaatt tataatttgc
    12421 caattgtctg aaagcatctg ctttcttttt gcatataaat tttgacatgt ttgtttcatc
    12481 aaggccaaaa aggttatggc tgattttacc atcctttgtt ttattatcac ctgataatgg
    12541 aatgtatact ttatcagcac tgacccccca ataaccatag gggtcttttt tttgattagc
    12601 tctgggaaaa aatatttttt tgttgttatt ttgatcttgt tttgaattta ataagtttat
    12661 aacctcttgg cctgcatcaa gtctttcggc tgtttggtct gaaatcatat ttagtttgtc
    12721 ttgcttatag gtatcgacac tgtaacctaa gcgtttttca aaaaggtagt ttatttctgt
    12781 atcttttgta acatccccgc cgggatacca gctgagatag gtatgagttg gttcctgtag
    12841 taaattgttt tttatactga tactagtatg ccctaaatgg gttatgcctc tatttcccca
    12901 gcaacgaaaa actaaatgtc tttcaagatt tttggctgta ccgatgccat gaaaacgctc
    12961 tgcggtattc aacattaatt tgatgtctct acctgtgaaa gagtgttgaa ttgagttttt
    13021 agctgcttca taggcggcaa acgcactttc tgcagatttt tcgctagcta atgtattgag
    13081 gaaaagcaat aaggattttt gattctctag tcgataattt tttagttgtt catttaatat
    13141 ctgtgaattt atctttgcat taatagtgct taatgttctg aaaaagagat cctttaggtt
    13201 gaatttctgt gctatcaata taggtccatg ctctgtatct tcaacgcgta taggcccttt
    13261 tgtattgctt gcgtcaatga aattgctaat tttgaggctc atactttatt aactctccat
    13321 tacttggtga ttgtggggat gtaaaatgta tttggcatat aatatattca attaataaat
    13381 ttagaaactt gagcctgtta acataatcaa attttctttt gctgtacata atatgtacct
    13441 cgtgagcata tgtagtgctc gtttcatcat gaaatcccac aagataaagt gcctgatgta
    13501 tcaggctcgg agtgttatag aaaaagagag aaccctgttg aataagtatc ttttttgttg
    13561 taaaacgagg tacaaaactg caatcaatgc cccatttatg actgaggata attaaatgca
    13621 cataactaat ttgggattgc atcaggtttc atttcaaagc ggagattcct ataaaggcgc
    13681 agaagaaacc ggaaagcaca aaggtgtaag cgtgatttca tatcaaagag ttaaaaatgg
    13741 agaaagaaat aaggggatcg aagcgcttaa tcgattatat ttacagaacc aaacaagctt
    13801 aacaggaaaa agccttttgt ttgcgcgtga cagagctgaa gtttattacg aggcaataaa
    13861 gttggcaggt ggtgatacgt caaaaattaa agccatgatg gaacgattag atacctataa
    13921 acttggtgaa gttaataaaa gacatattaa tgagcttaat aaagtaataa gtgaagagat
    13981 aagagcacag ctaggcatta aaaataaaaa ggaattgcaa acgaaaataa aacaaatatt
    14041 tactgattat ttaaataata agaattgggg accggtcgac aagaatatta gtcatcatgg
    14101 gaaaaattat ggttttcaat taacacctgc ctctcatatg aagataggga ataaaaatat
    14161 atttgtcaaa gagtataatg gaaaaggaat ttgttgtgct tctacgagag agagtgagca
    14221 tatcgcgaat atgtggcttt caaaagtggt ggatgacgag gggaaggaaa ttttttcggg
    14281 tatcagacat ggagttatat cagcctatgg attgaaaaaa aactcatctg aaagagctgt
    14341 tgctgctcgt aataaagctg aggagttagt aagcgcagca ttatatagca gaccagagtt
    14401 attatcacag gctttgtctg gtaaaacagt agatttaaag attgtttcga cttctcttct
    14461 gacgccgacc agtttaaccg ggggagagga gagtatgtta aaagatcagg tgaacgcatt
    14521 aaaagggtta aactctaaaa ggggggagcc aacgaaatta ttaattcgga atagtgatgg
    14581 tcttctgaaa gaagtaagcg ttaatctaaa agtggtgaca tttaactttg gtgtaaatga
    14641 attagcgcta aaaatgggct taggctggag gaatgttgac aagcttaatg atgaatcaat
    14701 atgttctttg ttgggggata atttcctcaa aaatggtgtg attggaggtt gggctgcaga
    14761 agcaatagaa aaaaatccac catgtaagaa tgatgtgata tatctggcta accagataaa
    14821 agagattata aacaagaagt tacaaaaaaa tgataatgga gagccgtata aattgtcaca
    14881 aagaatgact ctattggctt atactatagg tgctgtacct tgctggaatt gtaagagtgg
    14941 gaaggacaga acaggtatgc aggatgctga aattaaaaga gaaataataa gaaaacatga
    15001 aacaggtcag ttttctcaat taaatagtaa attatcctca gaagagaaaa gattattttc
    15061 tactattcta atgaatagtg gtaatatgga aatccaagag atgaatactg gtgttcccgg
    15121 aaataaagtc atgaaaaaat tgccgctatc ttcgttagag ctatcttatt ctgaaagaat
    15181 aggggaccca aaaatatgga atatggtgaa agggtattcg tcatttgtat aagaggaata
    15241 tatggaagat ttagcagatg ttatttgccg agccttgggt atccctttaa ttgatatcga
    15301 tgatcaagca atcatgcttg atgatgatgt gctaatatat attgaaaaag aaggagattc
    15361 aataaattta ttatgccctt tttgtgcatt gcctgaaaat ataaatgatc ttatatatgc
    15421 gttgagccta aattactcag aaaagatatg cttagctact gatgatgaag gcggaaatct
    15481 aattgcgcga ttagatctta ccgggataaa tgaattcgag gatgtatatg ttaatacaga
    15541 atattatatt tcgcgtgtgc gttggctgaa ggatgaattt gcgcgaagaa tgaaggggta
    15601 ttaatgtccc gtttcgtatt tattttgctg tgttttatcc catatttagg gagagctgat
    15661 tgttgggata aggctggtga aaggtacaat attccatcaa gtttgttaaa agcgattgcg
    15721 gaaaaagaat ccggatttaa taaatctgca gtcaatgtta ataacaatgg aagtaaagat
    15781 tatggtataa tgcaaataaa tgactttcat tctaaaagac ttagagaaat gggatattct
    15841 gaggaaatgc taattagtca tccatgtctt tctgtacatt atgcagcaaa gttattaaat
    15901 gagtttatga tgatgtatgg aagagggtgg gaggctgttg gagcctataa tgcaggaacg
    15961 tctccgaaaa agaaaaaaga aagattaaag tacgcagagg atatctatag acgctatttg
    16021 cggatagcag cagagagcaa gcagaataat cgaaggatat aagaggattt atgtctgagg
    16081 caaagaactc aaatcttgca ccattcaggt tattagtgaa actcacaaac ggtgtcgggg
    16141 atgagtttcc gttatattat gggaataact taattgttct cggacggacg atagaaacac
    16201 ttgagtttgg aaacgacaat ttcccagaaa atattattcc tgtaacagat agtaaatcgg
    16261 atggtataat ttatttgacc ataagcaaag ataatatctg tcaatttagt gatgaaaaag
    16321 gtgagcaaat tgatatcaat tcacaattta attcatttga atatgacggg atttcttttc
    16381 accttaaaaa tatgagggag gataaatcga gaggccatat actaaacgga atgtataaga
    16441 accactcggt gttttttttc tttgctgtta ttgttgtgtt aattataatt ttttccctct
    16501 ctttaaagaa agatgaagta aaagaaattg ctgaaattat agatgataag agatatggaa
    16561 tagtcaacac tggacagtgc aattatattc ttgccgaaac acaaaatgat gcggtatggg
    16621 cgtctgtagc cttaaataaa accggattta ccaaatgcag atatattctg gtgtcaaaca
    16681 aagaaataaa tagaattcaa caatatatca atcaacgatt cccatttatc aatttgtatg
    16741 tgttgaattt agtgagtgat aaagcggaat tactggtgtt tttaagtaaa gaaagaaata
    16801 gttcaaaaga taccgaactt gataaattga aaaatgctct gattgttgaa tttccgtata
    16861 tcaaaaatat taaattcaac tatcttagcg atcataatgc aaggggggat gctaaaggta
    16921 ttttcacaaa ggtaaatgtc cagtataaag agatttgtga aaataataaa gttacatatt
    16981 ctgtcagaga agaactgaca gatgaaaagc ttgagctcat taatagactg atttctgaac
    17041 ataaaaaaat atatggagat cagtatattg agttttctgt acttttgata gatgatgatt
    17101 ttaaaggtaa atcatatctt aacagcaaag acagttatgt aatgttgaat gataaacact
    17161 ggtttttttt tagataaaaa taagtgagga taaaatgagt gttacagtac cgaatgatga
    17221 ttggacattg agttcattat ctgaaacttt tgatgatgga actcaaacat tacaaggtga
    17281 actaacattg gcactagata aattagctag aaatccttcg aatccacagt tgctggctga
    17341 ataccaaagt aaattatctg aatatacatt atataggaac gcgcaatcca atacagtgaa
    17401 agtgattaag gatgttgatg ctgcaattat tcaaaacttc agataatagg gagcattcat
    17461 gaattacatt tatccagtca atcaggttga tattatcaaa gccagtgatt ttcaatctca
    17521 agagatatca agtctggaag acgtcgtgtc ggctaaatat agtgatatta agatggatac
    17581 agatattcaa gtatcacaaa taatggagat ggtaagcaat ccagaatcat taaacccaga
    17641 atctttggcc aagttacaga cgacgctctc aaattattca ataggagtat cattagctgg
    17701 cacgttagca agaaaaacag tttcggctgt tgaaacttta ttaaagtctt aatttatatg
    17761 attaggtata aaggttttat tttattcttg ttgctgatgt tgattggatg tgagcaacgt
    17821 gaagagttaa tttctaattt atctcaaaga caggcaaatg aaataatatc tgtgctagaa
    17881 cgccataata ttactgctag aaaagttgat ggaggtaaac aggggatctc ggtacaagtc
    17941 gaaaagggga catttgcatc ggcagttgat ttgatgcgca tgtacgattt gccaaatccg
    18001 gagagagttg atatctcaca aatgtttcct acagattcat tagtgtcttc tccaagagct
    18061 gaaaaggccc gtttatatag tgctattgag caacggctgg aacagtcttt agtttctatt
    18121 ggtggtgtta tttcggcaaa aatacatgtt agctatgatc ttgaagaaaa aaatatatct
    18181 tcaaaaccga tgcatatatc agtaatcgct atatatgact caccgaaaga gtctgaacta
    18241 ttagttagta atattaagcg atttttgaaa aacacctttt ctgatgttaa gtatgaaaat
    18301 atatctgtca tattaactcc gaaagaagaa tatgtttata caaatgtaca acctgttaag
    18361 gaagttaaat cggaattttt aacaaatgaa gtaatatatt tatttctcgg gatggctgta
    18421 ctagttgtca ttcttttggt atgggcattc aaaacagggt ggttcaagag aaacaaaata
    18481 tgataagaat ggatggaatt tataaaaaat atctttcaat aatttttgat ccagcgttct
    18541 atataaatag aaatcggttg aatttgcctt ctgaactgtt agaaaatggc gtaatcagaa
    18601 gtgagattaa taatctcata attaataaat atgatctaaa ttgcgatatt gaacctttaa
    18661 gcggggtaac cgctatgttt gttgccaact ggaatttact tccagctgtt gcttatttta
    18721 tcggtagtca agagtctcgg ttaattaatc atagtgagat ggttattagt tattatgggg
    18781 gcaaaatcag taagcaaggg gaggccgcta ttcgaagtgg tttttggcat cttattgcgt
    18841 ggaaggaaaa tatatctgtt ggtatatatg aaagaataaa cttattattt aatccaattg
    18901 ctttggaggg aaattatacg cctgtagaaa gaaatttgtc ccgattaaat gaaggtatgc
    18961 aatatgcaaa aaggcacttt accggtatcc agacatcatg cctatgatgg tgttgttata
    19021 aagagaattg aaaaggaatt atgtaaaacc ataaaagata gagacactga gagtaagaag
    19081 aaagcaattt gtgttataaa ggaggctacg aaaaaagctg aatcactgcg tatagatgct
    19141 gtatgtgatg gttatcaaat tggtatacag actgcttttg agcatattat tgactatatt
    19201 tgtgaatgga agttaaagca aaatgaaaac agaagaaata ttgaggacta tataactagt
    19261 ttgctttcag aaaatttaca tgatgagagg atcattagca ctttattgga gcagtggctt
    19321 agttcgttgc gtaatacagt aacagaatta aaagtggttt tgcccaagtg taatttagca
    19381 ctgagtaaaa agttagaact tgatctccat aaatatcgct cagatgtcaa gattatttta
    19441 aaatattctg aagggaataa ttatatattc tgtagtggta atcaagtagt ggagtttagt
    19501 cctcaggatg taatatcagg tgttaaaata gaacttgcgg aaaaattaac aaaaaatgat
    19561 aaaaaatatt tcaaagagtt agcacataag aaactgagac aaattgcaga agacttgtta
    19621 aaagagaatc ctgttaatga ttaatcaaat aaatgcaagc aatgctctgc agcaaagatt
    19681 aaatagtgaa gaatttgtta atttaaatga gagattatca tcttcgcaat catttgatga
    19741 ggatataatc tatgaaataa tgcaatattt ctcacagagt gaattaaatt ccatagataa
    19801 tgatgaactg cacaataaga ttgaacaatt attcaattca cgttttcctt atcttacagc
    19861 tgcacaaaaa agttcacttc ttaataaact catagatgca aatcaatatg tcgatttgca
    19921 tgagggattt tatgcatcat tgtccatata taacaatatt gatttctata ttaagactac
    19981 aaccttcgat tcattaatta gtgtctttga agcagggaga gaggcagatg attcgacatg
    20041 gtagtaataa gttgaaaata tttattttaa gtatattgct attaacactg agtgggtgtg
    20101 ctttaaagtc atcatctaat tctgaaaaag aatggcatat tgttcctgta agtaaggatt
    20161 atttttctat tccaaatgat ttattatggt cgtttaatac aaccaataaa agtataaatg
    20221 tttactctaa atgtattagt ggtaaggcgg tttatagttt taatgcaggt aaattcatgg
    20281 gcaactttaa tgttaaggaa gtagatgggt gcttcatgga tgcacaaaag atagctatag
    20341 ataaactatt ttctatgctg aaagacgggg ttgttttaaa aggtaataag ataaatgata
    20401 ccatccttat agagaaggat ggggaagtta aattaaaatt aattcgaggg atataattgt
    20461 attgtgagta aatataaagg tctaaataca agtaatatgt tttacattta ctctagtgga
    20521 catgaaccag ttaacgttga gcttgtaaaa gataaagaac gtaacataat tgagctggct
    20581 ccagcatgga agggtttttt tttgtccgaa atcaaaatat aaaattcagt gataatgtga
    20641 actatcatta tcgctttaac attaatagtt gtgcaaaatt tcttgctttt tgggactatt
    20701 tcagtggtgc tctagtagag catagccatg ctgaaaaatg tattcatttc tatcatgaga
    20761 atgatcttcg ggatagttgt aataccgaat cgatgcttga taagttaatg ttaagattca
    20821 tcttttcatc tgaccagaat gtaagtaatg cactggctat gatacgaatg actgagtcat
    20881 atcatttggt tctttatctt ctgagaacta ttgagaaaga aaaggaggta agaataaaga
    20941 gtctgacaga gcattatggt gtgtcggagg cttattttag atctttatgt agaaaagctt
    21001 taggggcaaa agtcaaagaa caactgaaca cttggcggtt agtaaatggt ttattagatg
    21061 tatttttgca caatcaaaca atcacttctg ctgccatgaa taatgggtat gcatcaacat
    21121 cccatttttc aaatgagata aaaacacgac taggcttttc tgcaagagaa ttatcgaata
    21181 ttactttttt agtgaaaaaa ataaatgaaa aaatttaata ttaaatcttt gactctcttg
    21241 attgtattgt taccattgat agttaatgct aataatattg actctcattt attagaacaa
    21301 aatgacatag cgaaatatgt tgctcaaagt gatacggtag gatctttttt tgaacgtttt
    21361 tctgcgttat taaattatcc tattgttgtt agcaaacaag ctgcaaaaaa gagaatatct
    21421 ggggagtttg atttatctaa tccagaagaa atgcttgaaa aactgacatt acttgtggga
    21481 ctcatatggt ataaagatgg aaatgcatta tacatatatg actctggaga attaataagc
    21541 aaagttattt tgctggagaa tatatcactt aattatctta ttcagtatct gaaggatgct
    21601 aacctttatg atcacagata tcctataaga ggaaacatta gtgataaaac attctatata
    21661 tctggtcctc cggcattggt tgaactggta gctaatacag caactttgct tgataagcag
    21721 gtttcttcta ttgggacaga taaagttaat tttggggtta taaaattaaa aaatacattt
    21781 gttagtgaca gaacttataa tatgagaggg gaggatattg tcatcccagg ggtggctact
    21841 gttgtagaac gtttattaaa taatggtaag gctctgagta acagacaagc ccaaaatgat
    21901 ccgatgccac cctttaatat tacacagaaa gtatctgagg atagtaatga cttttcattt
    21961 agttcagtta ccaatagttc tatattagag gatgtatcac ttattgcata tccagaaacc
    22021 aattctatat tagtcaaagg taatgatcag caaatacaga ttattcgcga tattatcact
    22081 cagttagatg tagccaaacg acatatagaa ttatcattat ggataatcga tattgataaa
    22141 tctgaattaa ataaccttgg tgttaattgg caaggaacgg cttcatttgg tgattcgttt
    22201 ggtgcgtcat ttaatatgag ctcatcagca tcgataagca ctttggacgg caataagttt
    22261 attgcttcag ttatggcatt aaatcagaaa aagaaagcaa atgtagtttc aaggccggtg
    22321 attttgacac aagagaatat tccggctata tttgataata acaggacttt ttatgtgtca
    22381 ttagttggtg agcgcaattc ttcgttggag catgttacgt atggaacatt gataaatgtt
    22441 atacctcgct tttcatctcg gggacaaatt gagatgtctt taacgataga ggatggaaca
    22501 ggaaatagtc agtcaaatta caattataat aatgaaaata catctgtcct tccagaagtt
    22561 ggaaggacca aaatcagtac aatagcgaga gttccacaag gcaaaagttt gttgataggt
    22621 ggttatacgc atgaaacgaa ctctaacgaa atagtatcta ttccttttct ctcctctatt
    22681 cctgtaatcg gtaatgtgtt caagtataaa acaagtaata tcagtaatat tgtaagggtt
    22741 tttctgattc aaccccgtga gataaaggag tcaagttatt ataatactgc agaatataaa
    22801 agcctcatat ctgaaagaga aatacaaaag actacacaaa taatcccatc tgaaactact
    22861 ttgctggaag acgaaaaatc attggtttca tacttaaatt actaactata aagtaggtga
    22921 tgtatgcttg atgttaaaaa tacaggagtt tttagctctg cattcattga taagttgaat
    22981 gcaatgacaa attcagatga tggagatgag actgctgatg cagagcttga ttctggcttg
    23041 gctaatagca agtatattga ctcatctgat gagatggctt ccgctctttc gtcatttata
    23101 aacagaagag accttgagaa actgaaagga acaaatagtg atagtcagga acgtatttta
    23161 gatggggaag aagatgaaat taatcacaag atttttgatt taaagagaac gttaaaagat
    23221 aaccttcctc tagatcggga ttttatagac agactaaaga gatattttaa agatccaagt
    23281 gatcaagtct tagcattaag ggaacttttg aatgaaaaag atcttactgc tgaacaagtc
    23341 gaattattaa ctaaaattat taatgagata atatcaggta gtgaaaaaag tgttaatgct
    23401 ggaataaatt cagctataca ggctaaatta tttggcaaca aaatgaaact tgaaccacag
    23461 cttttgcgtg catgttatcg tggttttatc atggggaaca tatcaacaac agatcagtat
    23521 atagaatggc ttggtaattt tggttttaat cacagacata caattgtgaa ttttgtagag
    23581 cagtcactga ttgtagacat ggattctgag aaaccgagct gtaatgctta tgagtttggt
    23641 tttgtgttat ctaaattaat tgcaattaag atgattagaa cttcagacgt aatttttatg
    23701 aagaaactgg aatcctcaag cttgctaaaa gatggcagtt taagtgcaga gcagctattg
    23761 ctaactttat tatatatttt tcaatatcca agtgaaagtg agcaaattct tacttctgtt
    23821 atagaagtat cacaagccag tcatgaggat tctgtagtgt atcaaacata tctatcttct
    23881 gttaatgaaa gtcctcatga tatatttaaa agtgaaagtg aaagagaaat tgcgatcaat
    23941 attctacgag agcttgtcac aagtgcatac aagaaagagc tttctagata acaggagata
    24001 aaagtgatcc agtcttttct taagcaagta agtactaagc ctgaattaat tatattagtg
    24061 cttatggtta tgatcattgc tatgttgatc ataccattac ctacatacct tgttgatttt
    24121 ttaatagggc ttaatattgt acttgctatt ctcgttttta tgggatcatt ttatattgaa
    24181 agaatattga gtttttctac attcccttcg gttttgttga taacgacatt atttcgtctc
    24241 gctttatcaa taagtactag tcgactaatt ttagttgatg cggatgcggg taaaattatt
    24301 actacatttg gccagtttgt cattggtgat agtctggctg taggttttgt tattttttca
    24361 atagtaactg ttgtgcagtt cattgttata acaaaaggat ctgaaagggt tgcggaggta
    24421 gctgctcgct tttctcttga cggtatgccg gggaaacaaa tgagcattga tgcggattta
    24481 aaggctggaa ttattgatgc cgcaggagct aaagaaagac gcagtattct ggaacgtgag
    24541 agtcaattgt atggttcatt tgatggagca atgaagttca ttaaaggtga tgcaattgct
    24601 ggcatcatta ttatttttgt caatttaata ggaggtattt ctgttggtat gagtcaacat
    24661 ggaatgtccc tatctggtgc attatctact tataccatac taactattgg tgacggattg
    24721 gtctctcaga ttcctgctct gttaatttct ataagtgcag gatttatcgt tacaagagtt
    24781 aatggtgata gcgataatat gggacgtaac ataatgtccc agatatttgg gaatcctttt
    24841 gttctcattg ttacatcagc tcttgccttg gcgataggga tgttgccagg ttttccgttt
    24901 tttgtttttt tcctgatagc agttactttg acggctttat tctattataa aaaggtcgta
    24961 gaaaaagaaa aaagtctgtc tgagtctgat tctagtggct atactggtac atttgatatt
    25021 gataatagcc atgactcatc tttggcaatg atagaaaatc tggatgctat tagttcagaa
    25081 accgttcctt taattttatt atttgccgaa aataagataa atgcaaatga tatggaaggt
    25141 cttattgaaa ggataagaag tcagttcttt attgattatg gtgtcaggct tccaactatt
    25201 ttatatagaa caagcaatga gcttaaggtc gatgatattg ttttgttaat aaatgaggtg
    25261 cgtgccgata gttttaatat atattttgac aaagtgtgta ttacagatga aaatggagat
    25321 atagatgctc taggtattcc tgttgtttca acttcatata atgaacgtgt tatttcttgg
    25381 gtagacgttt catatacaga aaatctaact aatattgatg ctaaaattaa aagtgctcaa
    25441 gatgaatttt atcaccagtt gtcacaagct ttattaaaca acataaatga gatttttggt
    25501 atacaagaaa caaaaaatat gttagatcag tttgaaaatc ggtatcctga tctattaaag
    25561 gaagtcttcc gacatgtgac tatacagaga atttctgagg tattacaaag attgcttgga
    25621 gaaaatattt ctgttcgcaa tttaaaactt attatggagt ctttggcgct ttgggctccc
    25681 agagaaaaag atgtcataac attagttgaa catgtccgtg catcactttc taggtatatt
    25741 tgtagtaaaa tagctgtttc tggtgagatt aaagttgtga tgctttccgg atatattgag
    25801 gatgcaataa gaaaagggat aaggcaaacc tctggtggct ctttcttgaa tatggatata
    25861 gaggtttcgg atgaggtaat ggaaacttta gcacatgctt tgagagaatt gagaaatgca
    25921 aaaaaaaatt tcgttctttt ggtatcagta gatatacgta ggtttgttaa aagacttata
    25981 gataacagat ttaagagtat actcgttata tcgtatgctg agattgatga agcatatacc
    26041 attaatgtat taaagactat ttagtgaggt ttaaatatga gtaacattaa tttagttcaa
    26101 ttagttagag atagtctttt cacgattggt tgtcctccat caattattac agatttagac
    26161 agccattcag cgattaccat ttctttggat agtatgcctg ctattaatat agctcttgtc
    26221 aatgagcagg ttatgttatg ggctaatttt gatgcaccaa gtgatgtaaa acttcagtca
    26281 tctgcttata acatattaaa tttaatgtta atgaacttta gttatagcat taatgagctg
    26341 gtagagcttc atcgttctga tgagtactta cagttaagag ttgttattaa agatgactac
    26401 gttcacgatg ggattgtttt tgcagagatt ctgcatgagt tttatcagag gatggaaatc
    26461 ttaaatgagg tcttataatc aatgagctat acaaaattgc tcactcaatt atcttttcct
    26521 aatagaatct cggggccaat cttggaaaca agtcttagcg atgtttcgat tggtgagatt
    26581 tgtaacattc aggctggaat tgaaagtaat gaaattgttg caagagctca ggttgtagga
    26641 tttcatgatg aaaaaacaat attaagcttg attggaaatt ctcgtggact ttcacggcaa
    26701 acgttgatta agcccactgc ccagtttctt catacgcaag tcggccgtgg attattggga
    26761 gcagtagtca atcctttagg ggaggttact gataagtttg ctgttacgga taacagtgaa
    26821 attctttatc gacctgtaga taatgctcct ccgctatata gtgaaagggc tgcaattgag
    26881 aagccttttt taacaggtat taaggttatt gattctttac tcacgtgtgg tgaaggacag
    26941 cgaatgggga tttttgcgtc agctggttgt ggcaaaactt ttctcatgaa tatgctcatt
    27001 gaacatagtg gtgctgatat atatgttatt gggttaattg gtgagcgagg tcgagaggtt
    27061 acagaaacgg ttgattattt gaaaaactct gagaaaaaaa gcaggtgtgt tttagtatat
    27121 gcaacttcgg attactcttc ggttgatcgt tgtaatgctg catatatagc cactgctata
    27181 gccgaatttt ttaggactga aggacataaa gtagcgcttt ttattgattc attaacaagg
    27241 tatgccagag cattacgtga tgtggcctta gccgctggag aatcacctgc cagaagaggc
    27301 tatccggttt cggtttttga tagcttaccc agacttcttg aaaggccagg aaagttaaag
    27361 gcaggtggct ctattactgc attttacact gttcttttgg aggatgatga ttttgctgat
    27421 ccattagctg aagaggtaag atccatttta gatggacata tatatttgag cagaaatcta
    27481 gcccaaaaag gacaatttcc tgcaattgat tccttaaaaa gtataagcag ggtatttaca
    27541 caggttgttg atgaaaaaca tcgtattatg gccgctgcat ttcgggagct actttctgaa
    27601 atagaagagc taaggacaat tattgacttt ggtgaataca aaccggggga gaatgcctct
    27661 caggataaaa tctacaacaa aatatctgtt gttgagagtt ttctgaagca agattatcga
    27721 ctcggtttta cttatgagca gacaatggag cttattggtg aaacaattag ataaggtttt
    27781 aaaaatcaaa gataaatatc aacggtctgt taaattaatt gaagcacaca tattaacact
    27841 aaaaaaaaaa ctctttgtat cgagatgtgg aggcattaga taaaaggatt atatacttcc
    27901 tgcaactgga gaatgactta gaacctgtag gcgctcagag cgttagtcag cttttcaata
    27961 caagacgtaa gatcgctata gtaaaaaagc atattatcca atatcagtct gaacgaattc
    28021 ttctaaaggg aagaattgaa gaaattcaaa aagatattga tgaggcaaat gcttctaaaa
    28081 gaaaactgct tcataaagag agtaaaatat gtaaacggat tggcttgatt aaaagaaata
    28141 actttgccaa acagttgata cttgatgagt tatcacaaga ggatatgaag tatggcatta
    28201 gataatataa acctaaattt tagtagtgac aaacagatag aaaaatgtga gaaactatct
    28261 tcaatagata atattgatag tctcgttttg aagaagaaga ggaaggttga aattcctgag
    28321 tactctttaa ttgcatctaa ctattttact attgataagc actttgaaca taagcatgat
    28381 aaaggagaaa tttatagtgg cattaaaaat gcgttcgaac ttagaaacga acgagcgaca
    28441 tattctgata ttccagaatc aatggccatt aaagaaaata ttttgatacc agatcaagat
    28501 atcaaagcaa gggaaaaaat aaatatcggc gatatgaggg ggatcttttc atataataag
    28561 agtggaaatg cagacaagaa cttcgaaaga agtcatactt cttctgtaaa ccctgataat
    28621 ctgctagaat ctgataatag aaatggtcaa attggtttaa aaaatcatag cttgtctatt
    28681 gataagaata ttgctgacat catttcttta ctaaatggaa gtgttgctaa atcatttgag
    28741 ctgcctgtaa tgaataaaaa tactgcagac ataaccccat ccatgtcatt gcaagaaaaa
    28801 tcaatagttg aaaatgataa aaatgttttt caaaaaaata gtgaaatgac ttaccacttt
    28861 aaacagtggg gggctggaca ttctgttagt atttcagtgg agtctggttc ttttgttcta
    28921 aaaccgtcag atcaatttgt aggaaataaa cttgacttaa ttttgaaaca agatgctgag
    28981 ggtaattaca gatttgatag cagtcaacat aataagggga ataaaaataa tagtacagga
    29041 tataatgaac agagtgaaga agaatgctaa gaattaaaca ttttgacgct aacgaaaaac
    29101 tacagatttt atatgcaaag caactctgcg agcgtttttc gattcagaca ttcaaaaata
    29161 aatttacagg cagtgaaagt ttagtcactc ttacttccgt gtgtggggat tgggtaattc
    29221 gtattgatac attatctttt ttgaaaaaaa aatacgaggt attttcagga ttttctacac
    29281 aagaatcttt actgcattta tcaaaatgtg tctttataga gtcgtcatct gtattttcga
    29341 ttccagaact gtctgataag attactttcc ggatcacgaa tgaaatccag tatgcaacta
    29401 ctgggagtca tttatgctgt ttttcctctt ctttaggtat tatttatttt gacaagatgc
    29461 cggtattacg taatcaagtt tctcttgact tattgcatca tcttttagag ttttgcttag
    29521 gttcatctaa tgtaaggctg gctactttaa aaagaattcg cactggtgat ataatcatag
    29581 ttcagaaact ttataattta ttattgtgta atcaagttat tattggggat tatattgtga
    29641 atgataataa tgaggcaaaa attaatctgt cagaaagtaa tggtgagtca gaacacacag
    29701 aagtttcttt ggcattattc aattatgatg atatcaatgt aaaagtggac tttattcttt
    29761 tagaaaaaaa tatgacaatc aatgaactaa aaatgtatgt agaaaacgaa ttattcaagt
    29821 ttcccgatga catagttaaa catgtaaata ttaaagtaaa tggttctttg gttgggcatg
    29881 gggaacttgt ttctattgag gatggttatg gtatcgagat tagttcttgg atggtaaagg
    29941 agtaatctca tgctgagtga catgtccctc atagctacac tttctttttt tacgctatta
    30001 ccatttttag ttgctgctgg tacctgttat ataaagtttt ctattgtttt tgtaatggta
    30061 cgaaatgcac ttggtttgca gcaggttcca tcaaatatga ctctcaatgg tattgcattg
    30121 attatggctt tgttcgtgat gaaacccatt attgaagctg gttatgaaaa ttatctaaat
    30181 ggccctcaaa aatttgatac aatatctgat attgtccgtt tttctgattc tggcttgatg
    30241 gaatataagc aatacttgaa aaagcataca gatcttgagt tagcacgttt tttccaaagg
    30301 tctgaagaag aaaatgctga tttaaagagt gcagagaata atgattattc attgttttcc
    30361 ctcttgcctg catatgcgct aagtgaaatc aaagatgcgt tcaaaatagg tttttattta
    30421 tatttgccat ttgttgttgt tgatcttgtt atttcaagca ttttacttgc acttggtatg
    30481 atgatgatga gtccaatcac aatatcagta ccaataaaac ttgtattatt tgttgcgctt
    30541 gatgggtggg gtatcctgtc taaggccttg attgaacaat atatcaatat tcctgcttag
    30601 atgatggagg taatgtgtct gatatagttt atatgggtaa taaggctctt tatttaatcc
    30661 ttatcttttc cttatggcca gtaggtatag ctacggttat tggattatct attggtttgt
    30721 tacagacagt gactcaactc caagagcaga cacttccttt tggtataaag cttataggtg
    30781 tctcaatatc tttgctactt ctttctggat ggtatggtga ggttttattg tctttttgtc
    30841 atgaaataat gtttttaatt aagagtgggg tttgatggac atttcaagct ggttcgaaag
    30901 tattcatgtg tttttaatac tcctgaacgg cgtttttttt agattggctc cattattttt
    30961 ctttcttcca tttttaaata acggtataat ttctccatct attagaatac ctgtgatttt
    31021 tcttgttgca tcaggattaa ttacttctgg taaggtagac ataggttctt ctgtttttga
    31081 acatgtttat ttccttatgt tcaaggaaat aattgttggc ctccttctct ctttttgctt
    31141 gtctcttccc ttttggatat ttcatgctgt tggtagcatt attgacaacc agcgtggggc
    31201 aacgcttagt agttcaattg atcctgccaa tggtgttgat acgtctgagt tggcaaaatt
    31261 tttcaatctt ttttctgcag ttgtatttct atacagtggt ggtatggtct ttattttaga
    31321 atccatacaa ttgtcttata atatatgccc gttattttct caatgttctt tccgtgtctc
    31381 aaatatctta acatttctga ctttattggc aagtcaggct gttattttag ccagtcctgt
    31441 tatgatagta ttgttactat cagaagtatt acttggtgta ttatcgagat ttgctccgca
    31501 gatgaatgct ttttccgtat cattaactat taaaagttta cttgcaatat ttattatttt
    31561 catctgttct tctactattt acttttctaa agttcaattt ttcctcggtg aacataaatt
    31621 tttcacaaat ctatttgtta gataaaatat tatggcaaat aaaacagaaa agccgacacc
    31681 taaaaaacta aaggatgccg caaaaaaagg acagtcattt aaatttaagg atttaacgac
    31741 tgttgttatt attctggtag ggacatttac tataatatca ttcttttcct taagtgatgt
    31801 aatgctctta tacagatatg taataattaa tgacttcgaa attaatgagg gtaaatactt
    31861 ttttgcagtg gttattgtct tttttaagat aattggcttc ccactttttt tctgtgttct
    31921 ttcggctgtg ttgccaacat tggttcaaac aaagtttgtt cttgcgacta aagctatcaa
    31981 gattgatttt tcagtattaa accctgttaa agggttaaaa aaaatattta gtataaagac
    32041 aataaaagaa tttttcaaaa gcattctgct tcttattatt ctagcattaa caacctattt
    32101 cttttggatt aatgaccgaa aaataatttt ttctcaggtg ttttctagtg ttgatggctt
    32161 atatcttatt tggggggggc tgtttaagga tataatatta tttttcttgg cattttctat
    32221 ttttgttatt attcttgact ttgtgattga gttcatttta tacatgaaag atatgatgat
    32281 ggataaacag gagataaaaa gagaatatat agagcaagag ggacactttg agacaaagtc
    32341 gagaaggcgt gagttgcata tcgagattct ttcagagcag actaaatctg atatacgtaa
    32401 ttcaaaatta gtggtaatga acccgactca tattgcaatt ggtatttatt ttaatccaga
    32461 aatagcgcct gcacctttta tttctctcat tgaaactaac cagtgtgcct tggctgtcag
    32521 aaaatatgca aatgaagttg gtataccgac tgtgcgtgat gtgaaattag ctagaaaact
    32581 atataaaaca catacaaaat atagttttgt tgattttgaa cacttggatg aagtcctacg
    32641 tcttattgtt tggcttgagc aggttgaaaa cactcattaa taagtaaagg agatgtgtta
    32701 tatgggagtt aatttctgta ataaaatagg tattgatcag tctgaatttg aaatagaatc
    32761 ttccatcatt aactccattg ctaatgaggt attgaaccca atatctttcc ttagcaataa
    32821 ggatataata aatgttttac tcagaaagat ttcatctgaa tgtgacctgg ttagaaaaga
    32881 catttatcgt tgtgctctgg agttggtcgt tgaaaaaaca cctgatgatt tataatcatt
    32941 cataataact cttttgctga tataaatatt tttgttgaag atatttatgt agtcacagga
    33001 tcaattgtag aaaattgtta tgtttttgtt aggaactgca ctaaataata tatgataaga
    33061 cagcaaaaac gactgaccat aatccttctt ttattaggag ttgataagag agactattca
    33121 tcctgtaatg ttaaaactct gttatatagt attcgcgatt atgcaaagtc tgtaaatgat
    33181 catgagatac taaccgagag taatcgttta ttatctcatt gcatttcaga tagtaacgga
    33241 gctttcttta aaagtagtaa atacgttccc cttaagtact tacgtaaaag gagaatagca
    33301 cgaaagatac caaatgatta gcactttgct agtaagcttg ctggtaaact atattgaccg
    33361 ttgggtttgt tttgagactg taatttaaat tgtgtaattg cctgtttttg atatgtttac
    33421 tccaacaacg gagacaggta aattatggac gaaaagaagc ttaaagccct tgcggctgaa
    33481 ctggctaaag gcctcaaa
//
LOCUS       DADMEQ010000032        33453 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.032, whole genome shotgun sequence.
ACCESSION   DADMEQ010000032 DADMEQ010000000
VERSION     DADMEQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33453
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.032"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(1..1606)
                     /locus_tag="JNZ05_001580"
                     /pseudo
     CDS             complement(1..1606)
                     /locus_tag="JNZ05_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase"
     gene            complement(1784..2695)
                     /gene="gsiD"
                     /locus_tag="JNZ05_001581"
     CDS             complement(1784..2695)
                     /gene="gsiD"
                     /locus_tag="JNZ05_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HAY8564854.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(2698..3618)
                     /gene="gsiC"
                     /locus_tag="JNZ05_001582"
     CDS             complement(2698..3618)
                     /gene="gsiC"
                     /locus_tag="JNZ05_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HAY8564855.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(3636..5174)
                     /gene="gsiB"
                     /locus_tag="JNZ05_001583"
     CDS             complement(3636..5174)
                     /gene="gsiB"
                     /locus_tag="JNZ05_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HAY8564856.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDSGFSFED
                     ADLQ"
     gene            complement(5194..7065)
                     /gene="gsiA"
                     /locus_tag="JNZ05_001584"
     CDS             complement(5194..7065)
                     /gene="gsiA"
                     /locus_tag="JNZ05_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HAY8564857.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(7052..8017)
                     /gene="iaaA"
                     /locus_tag="JNZ05_001585"
     CDS             complement(7052..8017)
                     /gene="iaaA"
                     /locus_tag="JNZ05_001585"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY8564858.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMESVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            8221..9456
                     /gene="moeA"
                     /locus_tag="JNZ05_001586"
     CDS             8221..9456
                     /gene="moeA"
                     /locus_tag="JNZ05_001586"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HAY8564859.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            9456..10205
                     /gene="moeB"
                     /locus_tag="JNZ05_001587"
     CDS             9456..10205
                     /gene="moeB"
                     /locus_tag="JNZ05_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HAY8564860.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(10384..11046)
                     /gene="fsa"
                     /locus_tag="JNZ05_001588"
     CDS             complement(10384..11046)
                     /gene="fsa"
                     /locus_tag="JNZ05_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY8564861.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            11177..12076
                     /locus_tag="JNZ05_001589"
     CDS             11177..12076
                     /locus_tag="JNZ05_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAY8564862.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            12082..14514
                     /locus_tag="JNZ05_001590"
     CDS             12082..14514
                     /locus_tag="JNZ05_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HAY8564863.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHYELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMAAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            14660..15475
                     /gene="ybiV"
                     /locus_tag="JNZ05_001591"
     CDS             14660..15475
                     /gene="ybiV"
                     /locus_tag="JNZ05_001591"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HAY8564864.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            15626..16891
                     /locus_tag="JNZ05_001592"
     CDS             15626..16891
                     /locus_tag="JNZ05_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HAY8564865.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(17132..18724)
                     /locus_tag="JNZ05_001593"
     CDS             complement(17132..18724)
                     /locus_tag="JNZ05_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HAY8564866.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEGFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            18943..19863
                     /gene="ldtB"
                     /locus_tag="JNZ05_001594"
     CDS             18943..19863
                     /gene="ldtB"
                     /locus_tag="JNZ05_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY8564867.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(19921..21039)
                     /locus_tag="JNZ05_001595"
     CDS             complement(19921..21039)
                     /locus_tag="JNZ05_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HAY8564868.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFQTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGMQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(21036..21503)
                     /locus_tag="JNZ05_001596"
     CDS             complement(21036..21503)
                     /locus_tag="JNZ05_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MntR"
                     /protein_id="HAY8564869.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            21689..21817
                     /gene="mntS"
                     /locus_tag="JNZ05_001597"
     CDS             21689..21817
                     /gene="mntS"
                     /locus_tag="JNZ05_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HAY8564870.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            22089..23672
                     /locus_tag="JNZ05_001598"
     CDS             22089..23672
                     /locus_tag="JNZ05_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAY8564871.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYKTFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(23720..24235)
                     /gene="ompX"
                     /locus_tag="JNZ05_001599"
     CDS             complement(23720..24235)
                     /gene="ompX"
                     /locus_tag="JNZ05_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HAY8564872.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGLNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(24288..24353)
                     /locus_tag="JNZ05_001600"
     CDS             complement(24288..24353)
                     /locus_tag="JNZ05_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564873.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            24588..25475
                     /gene="rhtA"
                     /locus_tag="JNZ05_001601"
     CDS             24588..25475
                     /gene="rhtA"
                     /locus_tag="JNZ05_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HAY8564874.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            25774..26277
                     /gene="dps"
                     /locus_tag="JNZ05_001602"
                     /gene_synonym="pexB"
     CDS             25774..26277
                     /gene="dps"
                     /locus_tag="JNZ05_001602"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HAY8564875.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            26681..27427
                     /gene="glnH"
                     /locus_tag="JNZ05_001603"
     CDS             26681..27427
                     /gene="glnH"
                     /locus_tag="JNZ05_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HAY8564876.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEVYKKWFSTEPK"
     gene            27566..28225
                     /gene="glnP"
                     /locus_tag="JNZ05_001604"
     CDS             27566..28225
                     /gene="glnP"
                     /locus_tag="JNZ05_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HAY8564877.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            28222..28944
                     /gene="glnQ"
                     /locus_tag="JNZ05_001605"
     CDS             28222..28944
                     /gene="glnQ"
                     /locus_tag="JNZ05_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HAY8564878.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            29366..31429
                     /gene="ybiO"
                     /locus_tag="JNZ05_001606"
     CDS             29366..31429
                     /gene="ybiO"
                     /locus_tag="JNZ05_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HAY8564879.1"
                     /translation="MLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQK
                     VTEVSRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIR
                     LCALPLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNA
                     GSRTIAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLS
                     SLIGYGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAE
                     HSLAFFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAA
                     FVSGMFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAW
                     GLFDFWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSA
                     RTRTLLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDI
                     ITGVFIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANF
                     VRGIGSVVANYDVDRHEDADKANQALKDAVTELMENEEIRGLIIGEPNFAGIVGLSNT
                     AFTLRVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPP
                     GEPTL"
     gene            complement(31426..32352)
                     /gene="rlmF"
                     /locus_tag="JNZ05_001607"
     CDS             complement(31426..32352)
                     /gene="rlmF"
                     /locus_tag="JNZ05_001607"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HAY8564880.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            32628..32888
                     /locus_tag="JNZ05_001608"
     CDS             32628..32888
                     /locus_tag="JNZ05_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY8564881.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            33153..>33453
                     /locus_tag="JNZ05_001609"
     CDS             33153..>33453
                     /locus_tag="JNZ05_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HAY8564882.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         8234 a         8910 c         8232 g         8077 t
ORIGIN      
        1 tggcgaatat tacgcgcaat ccagatattc tcttctattt cgttgagttt cgcgtcatca
       61 cagaaaataa actcggagaa catatggtca cggatactgg aaatcgccag tttcagtttt
      121 tctgcgtaac tccaggcgtt tccccctgca aaggttcaca aatggcagac ttatggatgc
      181 gcagattgcg gtagccaaag ctgctgtcaa aatcgtttac catcgctatt ttacgggtaa
      241 atgtctgtac attttcccga cacagaagta aatagagatc ggcattgaat gtcacgatat
      301 cgcagtattg cgcatatttc tcggcaattc gctgacacaa tttcctgacg attttattgc
      361 gttcatcaac acaaaacacc gcttccacgg cttccagacc acacagtttg atgatcccca
      421 cagccagata ctgattattg ttggactcaa taatcgccat atttttaata aaaccgcttt
      481 cctgatcgta agagatcagt gagagcaatt tatttgaccg ttcaatttcg gcatctttca
      541 tttccgcgat cgaatttttg atgctgataa tctctttcgc atcgccttcg cgaattttca
      601 gagcctcgat ctgatccgtt gagtcgggca aaaacgccag cgtattaacc agctcggtca
      661 tttcagaaaa ttgccgacgg aacacacgag tggtcatgaa atataccacc agcaacacga
      721 taataatgaa cgcaaacggt agataaaaaa gtttatctga tgcgttcaat aaattaccgc
      781 caggataata aaaatagatt tcgaacaatg cattatttgg cactgctgat gtctttgcaa
      841 tatactccgt atcgacagaa tcaataatat tataattacc ttctctatta taaataatca
      901 gtttttcacg agcgagttga ttcttggcaa tgatctcatg accttgtttc aaaaatagaa
      961 cttcatcatt agcgctgatt tgatcaagat actcgttcag ataccgccag tttaaatcga
     1021 catgaatttt atctaccgct aacaaatcgt gatctttgcc gttgatacgt agcgtaaaag
     1081 agatacattt tttacccgga cgatcaaagc aattgtatgc cggattccag ctgttgttag
     1141 agtcatctga agccaccgac cacggtcgtt tcgatgggtt aaaatcggca aaaataaccc
     1201 gccctgactc gtcgacaagc ggtccattta cttgttcctg atgataaaca acgattttat
     1261 cattcgccct ccgcgaaaag agataatatt tattatcatc aagtaccagt cccaccgagt
     1321 ctaaaaatgg catcatttcc attttcagaa tcacggcgcg ttgaatctcc tctgtatttt
     1381 ttaataaata cagtgagtcc tttaatgtca gggccgaacg ttcaatatct ttaaagaaat
     1441 agccaacgtt tttctgaata agttcaaagc ggctatcggc acgttttacc gcgcgttccg
     1501 ataccagttg atagttaatc atgaaaaaaa gaccaataaa tgacagtgct gccaataaaa
     1561 ataaaattat cagccttatc tttatctttt cgtataaact cagcatgtta atacatttaa
     1621 caaaataact atctgaacgc tgtgccgctg caaaaatagt cccttgctgc ggctttttaa
     1681 ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta gggcaataat
     1741 ttcctgtgag gtgattaccc tttcaagcaa tattcaaacg taactatcct ttaattttcg
     1801 gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg gtcagaaaaa
     1861 tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct cgtgcctcat
     1921 tgagcattgc tccccactct ggtgtcggcg gctgcgcacc taggccgaga aatgacaggc
     1981 tggcggcaga gataatcgag gtgccaatgc gcatggtgaa aaacaccacg atagaagaga
     2041 cggtcccagg caggatatga cgcaataaaa tggtcatatc gctggcacca atactgcgtg
     2101 ctgactcaat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc aggcgggcaa
     2161 acgcggggat ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg cttcccaaca
     2221 ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca tcgcaaatgc
     2281 gcatgatcag ccgatcccac cagccttcat aatatccagc caacaagccc agcaacgtcc
     2341 ctatcgccac accgataaac acggcaaaca cgcccgccgc cagcgagatt tgcgcaccaa
     2401 ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag tgctgcaaag
     2461 aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag ggagcgatcc
     2521 agcgtgcaaa aatggccacc acaatcaata aaataacgaa taatgcggcg gtcatcgcca
     2581 tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgtacc tggtcaggtt
     2641 tgaccagtgg catggcgttt aacaccgcct gacgtcgcca gttaaatagt cgcatcctta
     2701 cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta agttgataag
     2761 aataaattcc agcgagaaaa gcagaatttc cgcctgaatc accgggtaat cgcgcatttc
     2821 tacagagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct ccaccacgat
     2881 agaacctccg agcaaaaagc caaactgtaa gcccatcatg gtcactaccg ggatcatcgc
     2941 gttgcgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg ccctcgcggt
     3001 acgcatataa tcttcgctta aaacgtcgac aaatgacgca cgggtaaagc gcgccatcac
     3061 ggcggccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc aactgtctgc
     3121 tcccacagta ggcagccagc ccagttcaac ggagaatacc tgaattaaaa acatccccag
     3181 cgcaaatgcc ggaaacgaga tccccgacac cgcaatggtc atactcaatc gatccggcca
     3241 acgattacgc cagacggcgg cgataattcc cgccgccata ccaaatatca ccgcccagac
     3301 catactggtt atggtcagcc acagcgttgg cataaagcgg ctggcaatct catcggcaac
     3361 cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc tgatatagtg
     3421 ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca gttctataac
     3481 ctgcgcatca gcttcgggcc cggcaatcaa tcgcgccgga tcgccgggca gcatgtggac
     3541 aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca gtaatcgttt
     3601 gataacgtaa ttaagcattc cactccctgc gttgcttatt gcaaatccgc gtcttcaaag
     3661 ctgaagccgc tgtctggcat gatccaaaaa ccggtcaggt ttttactgtg tgccgacacc
     3721 agtttttcta ccaccagcgg gatccacggc gattcttgcc agatgatatc ctgcgccgcc
     3781 ttatataagc gggtcttttc cgccggatca ttcgttttca gtgcctgagc caggaagtca
     3841 tccacctgtt tattgctgta aaacgcggta ttaaacagcg tcggtggcca gttttgcgag
     3901 gcaaacagcg gcgatagtgc ccagtcggct tcgccggttg aagccgacca gccagtgtag
     3961 aacatccgca cgccgctctc tttttgccct ttaccttcaa cttctgccgc ccgctgtccg
     4021 gcatccatcg cggtcacctg ggctttaatc ccgacctgcg ctaactgctg ctgggtaaat
     4081 tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt actgaaaccg
     4141 ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta aggccacggt
     4201 ttataacttt gcgcgtaggc gatacttggc ggtaccacac cagtagctgg cgttgcatag
     4261 cccgcaaagg caactttcac cagcgccgga cggttaatgg cgtaattcag cgcctcacgg
     4321 accttcgggt tatcgaacgg cttttgcgtc acgttcatac tgatataacg ctgcataatt
     4381 gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgtggc ctgctcgtag
     4441 ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg ggtgttgtta
     4501 tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg ccagtaaccc
     4561 gcgaattttt tcaccttcac aaaatcggtc tgattccagg tatccagttc atacggtccg
     4621 gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgt cggtgaaatc
     4681 atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaacggctg tttgagggta
     4741 atctttaccg ttgtcggatc gatcgcctcc gttttggcaa tattcttata caggttatag
     4801 cgtttaagat gattcgccgg atcgctggcc cggtccagat tcgcttttac cgccactgcg
     4861 ttgaaatcgg tgccatcctg gaatttaatt ccttcccgca atttcacggt gtaagtaatg
     4921 ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat ctctttatcc
     4981 agaccgaaca gcccctggta aaacgatttc gctacggcct gagataacgt gtcattcgcg
     5041 tcatacggat cgagcgtggt gaaattcgat cctaccgcca ccaccacatc tttggcagcg
     5101 aatgcacaag atgccatcaa cgctgtcgca atgcccagcg ccactaaccc actacggtgt
     5161 acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg cgtattctga
     5221 ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacgg ctgccacctc
     5281 ttcgccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac gctgcggtcg
     5341 ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat aaggatgctg
     5401 cgggttttcg aagaccgcgc accgtgaacc aatttcaaca atttgcccga gatacatcac
     5461 cgccacacga tgactaatcc gctctaccac agccatatcg tgggagataa acagatacgc
     5521 aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc gaatagaaac
     5581 atccagcgcc gaaacggctt cgtcggcaat gatcactttt ggattcaatg ccaacgcgcg
     5641 agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac gccaggcatg
     5701 ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg cgtctttacc
     5761 tggcaataaa ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg tctgacgtgg
     5821 gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcccggcgca atgcctgaag
     5881 tttgccaggt gacaaggtat cgattcgctg accgttaaag ataatttcgc cgccctgcga
     5941 ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag actcgcccac
     6001 cagcgatagc gtttcgccag gccagagatc aaaactgact ttctcaacgg catgcacttc
     6061 ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa gattacgcac
     6121 ccgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atggggggtt cctgtttcgc
     6181 tggatgttca agcgatatca atgggaaacg tcggggataa tctaaccctt tcatcgcacc
     6241 aagttgcgga acagcagcta acagcgcacg ggtgtaagga tgttgcggtg catgaaaaat
     6301 ctgttcgacg gtacccgttt ccaccgcctc gccctgatac atcaccagta cccgatcggc
     6361 aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg acatctcttt
     6421 ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca gcgcggtggt
     6481 tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa tcatcactcg
     6541 ctggcgcatc ccgccagaga gttgatgcga ataacgtgaa agaatggttt gtgcctccgg
     6601 aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac gactggcgtt
     6661 ctgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaatactg ggttcagcga
     6721 tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgcacatggc gcatttgtgc
     6781 agcgctctgc tcgctaagtt caatcacatc acgactgcgc cgccgcaaca gcattttatc
     6841 gcactgtact aaaccgcccg cctgttccaa cagacgcatc aatgccagcg cagtcactga
     6901 cttaccggag ccggattcgc ctacaattgc cagcgtctca ccgcgttgca gactaaaaga
     6961 gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat taagattttc
     7021 aaccgccagc acattaccgg catcaagttc atcactgtgt ggcaacggtg tccccttttt
     7081 cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga tacattcctt
     7141 cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag ccaccgctac
     7201 cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg agacttaatc
     7261 cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg aagacttcgc
     7321 ctgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg gcacccacta
     7381 agggactatc gccaactcgt ccgggtaatt tattggtcat tccgcctgtg gacgtggctg
     7441 ccgccagatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt ttctgttttt
     7501 catccagtgg cgcaccgcta tggtcgagga ctgttgcccc ttcctcgcgc gctgccagta
     7561 gttgttcata acgcaaaggc gtggagaaaa tctccggtga gacgctctcc atgccatgag
     7621 caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt tgctccatca
     7681 ccagccgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc gcaccggctt
     7741 tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca cgcgtaaaga
     7801 cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc gcttccgtca
     7861 ccacatccag cgcactttcg ccggcttcca gcattttctg cccggtttca acaatggcag
     7921 acaacgcctc gatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg ctaattgcac
     7981 ctgcgccacc atgaattgca atgactgctt tgcccatagt attcgtccat taagggtggc
     8041 tcgccacttt tgctataaat atcagaattt ttatgaatta ctcattcaat ttttgagtat
     8101 agaaagatgg atatggcatg taaaggcagg agccaacgct acgcccgagg tggcatacag
     8161 tctatggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag gagtgttttc
     8221 atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat gctttctcgc
     8281 gttaccccac tgaccaccca ggaaacgctg ccactggttc agtgttttgg tcgtattctg
     8341 gcgagcgatg tcgtttcgcc actggatgtc ccggggtttg ataactccgc tatggacggc
     8401 tacgcggtgc gtttagccga tattgcctcc gggcaaccgc tgcccgttgc cggtaaatcc
     8461 tttgccggtc agccatacca tggtgaatgg cctgcgggta cctgcattcg tattatgacc
     8521 ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac tgaacaaacg
     8581 gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg ccgtcgcggt
     8641 gaagatatct ctgcaggtgc ggttgttttc ccggcgggaa ctcgcctgac taccgcagag
     8701 ctgccagtaa ttgcttcact ggggattgcc gaagttccgg tgattcgtaa agtgcgtgta
     8761 gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccgctggg cgacggccaa
     8821 atctacgaca ccaaccgtct cgccgtacac ctgatgttag aacagctggg ttgcgaggta
     8881 atcaacttag gaattatccg cgacgatcca catgccctgc gcgccgcatt tattgaagcc
     8941 gacagccagg cggatgtagt gatcagttcc ggcggtgttt cagtgggtga agcggattac
     9001 accaaaacga ttcttgaaga gctgggggag atcgccttct ggaagctggc gattaaacca
     9061 ggtaaaccgt tcgcattcgg taaactcagc aatagctggt tctgcggcct gccgggcaac
     9121 ccggtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc aaaactaagc
     9181 ggcaacaccg ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc ccgcctgaaa
     9241 aaaacgccag gacgtcttga tttccagcgc ggcgtgctgc aacgcaacgc cgacggtgaa
     9301 cttgaagtga cgaccaccgg acatcagggt tcacatatat ttagctcctt tagtctcggc
     9361 aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg acgtgggcga atgggtggaa
     9421 gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatggc ggaactcagc gatcaggaga
     9481 tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt caggaggcgc
     9541 taaaagattc tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca gcttcgcagt
     9601 atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgacacg gtttcgctct
     9661 cgaatctgca acgccagaca ctgcacagtg atgccacggt cgggcaaccg aaggtggaat
     9721 ctgcccgtga cgccctgacg cggatcaacc cacatatcgc gattacgcca gtcaatgcac
     9781 tgctagatga cgcagaactt gcagcattga ttgctgaaca cgatctggtg ctcgactgta
     9841 cggataacgt tgcggtacgt aatcaactga acgcaggctg ttttgccgcg aaggtgccgc
     9901 tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact tatcaggacg
     9961 gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga gaacgcgtta acctgcgtag
    10021 aagcaggcgt gatggcaccg ttgatcggcg taattggttc gttgcaggca atggaagcga
    10081 tcaaactgct ggcaggttat ggcaaaccgg ccagcgggaa aatcgtcatg tacgatgcga
    10141 tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtgag gtgtgcgggc
    10201 agtaattgcc acctccaggc ctgataagac gcgacaagcg tcgcatcagg catttgctcc
    10261 agacgccaga tgcggcgtga acgccttatc tgccctacgg ttcgggcaca gatttccaag
    10321 cgcggcaggc atcggtgcac tccgccacgt catcagcaat ttatgaggat gcagaacgtc
    10381 cggttaaatc gacgttctgc caaacgctcc ctgccagtcc tgctcaaact tcgccacagc
    10441 ggcttccacc gccggatagc taatcatctg ttgtgccaca tccagtggca gagtaattga
    10501 ttcacatccg gccagtaagc agtccagcgc ctgacgcggg gttttgaaac tcgctgccag
    10561 cactttcgcc tgcggcgcat gcattttcaa taactggtgt aagtcggtca cagtctgaat
    10621 gccgctaccg ccctgagcat caatacgatt aacgtaaggc gcaacatatt ccgcacctgc
    10681 cagcgccgac agcagccctt gtgccgcgcc atataccgcg gttcccagcg tcggaatccc
    10741 ttccgctttt aacatcttaa tcgctgccag cccctcggct gtcaccggaa ctttcaccac
    10801 gatatccgca ataatagaac gcagcttaag cgcgtcattc accatccctt cagcagtggt
    10861 agccattacc tgggcaaaca gacgcccctg accgcccatc gcttcatgaa gttgcggaag
    10921 cacaacatcc agcggttttt tacccgcggc gataatgctt gggttagtgg tcacacccgc
    10981 cagcggaaaa atacgtgaca gcgccttcac cgcaacaacg tctgaagtat ccagatacag
    11041 ttccataacc atcctcaaaa tgtacttttt gtctgaatgt agactatcac gggaaaactc
    11101 tctgaacagt tgacgcacat caatctaact ttcattcgaa agtattttaa tgtttgaacg
    11161 aaacagagag gcaactatga ttttcaatat tcagcgctac tcgacccatg atggccccgg
    11221 tatccgcacg gtcgtatttc ttaaaggctg ttcgctgggc tgccgctggt gtcagaaccc
    11281 ggaaagccgc gcccgtacgc aggatctgct gtatgacgca cgactgtgtc tggaaggctg
    11341 cgagctgtgc gctaaggccg cgccggaagt gattgagcgc gcgctgaatg gtttgcttat
    11401 tcatcgggaa aagttaaccc cggagcatct gacggcgtta accgactgct gtccgacaca
    11461 ggcgttaacc gtgtgtggtg aagtgaaaag cgttgaggag atcatggcga ccgttctgcg
    11521 cgataaaccg ttttacgatc gcagcggcgg cggcttaacg ctttcgggtg gtgaaccctt
    11581 catgcagccg gaaatggcga cggcgctgct gcaagcctgc cacgaggcag gcattcatac
    11641 tgcggtagaa acctgtctac atgtgccgtg gaaatatatc gccccttctc tgccctatat
    11701 cgatctgttt cttgccgatt taaaacacgt tgccgacgcg ccgtttaaac agtggaccga
    11761 cggtaacgcc gccagggtgc tggataacct gaaaaaactc gccgcagcgg gcaaaaaaat
    11821 cattatccgc gtgccgctga ttcagggctt taatgccgac gaaacctctg taaaagccat
    11881 taccgatttt gccgccgacg agctgcacgt tggcgaaatt cattttctgc cctaccacac
    11941 gctgggcatc aacaaatatc acttacttaa tctgccctat gacgccccgg aaaaaccgct
    12001 tgatgcgcca gagctgctcg attttgccca gcagtatgcc tgccagaaag ggttaaccgc
    12061 gaccttacga ggataacaac catgaccaca ctgaaactgg acacgctcag cgaccgcatt
    12121 aaagcgcaca aaaatgcgct ggtgcatatt gtgaaaccgc cagtctgtac cgagcgcgcg
    12181 cagcactata ccgagatgta tcaacaacat ctcgataagc cgatcccggt acgtcgcgcg
    12241 ctggcactgg cgcatcacct ggcgaatcgc accatctgga tcaaacacta tgagttgatc
    12301 attggcaacc aggcaagcga agttcgcgcc gcgccgatct tcccggaata tactgtctcg
    12361 tggatcgaaa aagagattga tgatctggca gatcgtcccg gtgctggctt tgcggtgagc
    12421 gaagagaaca aacgtgttct gcatgaagtg tgcccgtggt ggcgcggtca gacggtacag
    12481 gatcgctgct atggcatgtt taccgatgag caaaaaggtc tgctggcgac cggcatcatt
    12541 aaagcggaag gcaatatgac ctccggcgat gcgcacctgg cggtcaattt cccgctgctg
    12601 ctggaaaaag ggcttgatgg tctgcgtgag aaagtagcgg aacgtcgctc gcgcatcaat
    12661 ctgacggtgc tggaagattt gcacggcgaa cagttcctga aagcgattga tatcgtgctg
    12721 gtggcagtca gtgaacacat tgaacgtttc gctgccctgg cgcgtgcaat ggccgcgacc
    12781 gaaacccgcg aaagccgtcg cgatgaactg ctggcgatgg cagaaaactg cgatcttatc
    12841 gcccaccagc cgccgcagac tttctggcag gcgctgcaac tgtgttactt catccagttg
    12901 attttgcaga ttgaatctaa cggtcactcg gtgtcgtttg gtcgtatgga tcagtatctc
    12961 tacccgtact atcgccgcga cgttgaactg aaccagacgc tggatcgcga acacgccatc
    13021 gaattgctgc atagctgctg gttgaaattg ctggaagtga acaaaatccg ctccggctcc
    13081 cactcaaaag cctctgcggg aagtccgctg tatcagaacg tcaccattgg cggacaaaat
    13141 ctggttgatg gtcaaccaat ggacgcggtg aatccactct cttacgcgat cctcgaatcc
    13201 tgcggtcgcc tacgttccac tcagcctaac ctcagcgtgc gttaccatgc aggaatgagt
    13261 aacgatttcc tcgacgcctg cgtacaggtg atccgctgcg gcttcgggat gccggcattc
    13321 aacaacgacg aaatcgtgat cccggaattt attaaactcg gtattgaacc gcaggacgct
    13381 tatgactacg cagcgattgg ttgtatagaa accgccgtcg gtggcaaatg gggctatcgc
    13441 tgtaccggca tgagctttat caacttcgcc cgcgtgatgc tggcggcgct ggaaggcggg
    13501 cgtgatgcca ccagcggcaa agtgttcctg ccacaagaaa aagcgttgtc ggcaggtaac
    13561 ttcaacaact tcgatgaagt gatggccgcg tgggatacgc aaatccgtta ctacacgcgt
    13621 aaatcaatcg aaatcgaata tgtcgtcgac accatgctgg aagagaacgt gcacgatatt
    13681 ctctgctcgg cgttggtgga tgactgtatt gagcgagcga aaagtatcaa gcaaggcggc
    13741 gcgaaatatg actgggtttc tggcctgcag gtcggtattg ccaacctcgg caacagcctg
    13801 gcggcagtga agaaactggt gtttgaacag ggcgcgattg gtcagcaaca gctggctgcc
    13861 gcactggcgg atgacttcga tggcctgact catgagcaac tgcgtcagcg gctgattaac
    13921 ggtgcgccga agtacggcaa cgacgatgat accgtcgata cgctgctggc tcgcgcttat
    13981 cagacctata tcgacgaact gaaacagtac cataacccac gctacggtcg tggtccggtt
    14041 ggcggcaact attacgcggg tacgtcgtct atctccgcta acgtaccgtt tggcgcgcaa
    14101 actatggcaa cgccggacgg gcgtaaagcc cacaccccgc tggcagaagg cgctagtccg
    14161 gcctctggta ctgaccatct cggccctact gcggtcattg gctcagtggg taaactgcct
    14221 acggcagcga ttctcggcgg cgtgttgctc aaccagaaac tgaatccggc aacgctggag
    14281 aacgaatctg acaagcagaa actgatgatc ctgctgcgta ccttctttga agtgcataaa
    14341 ggctggcata ttcagtacaa catcgtttcc cgcgaaacgc tgctggaggc gaaaaaacat
    14401 ccggatcagt accgcgatct ggtagtgcgt gtcgcgggtt attccgcctt cttcaccgcg
    14461 ctctctccag acgctcagga cgatatcatc gcccgtactg aacatatgct gtaatctctt
    14521 cggcccggct gcggcgtcgc cgggctaaat cattcacatc atcaatttca tccttacttt
    14581 cattcgaaat ataatttgtg ctctgcgtca cattgttatt acactgttac ggtttcattt
    14641 tcgagccagg agcacaccta tgagcgtaaa agttatcgtc acagatatgg acggtacttt
    14701 tcttaacgac gccaaaacgt acaaccaacc acgttttatg gcgcaatatc aggaactgaa
    14761 aaagcgcggc attaagttcg ttgtcgccag cggtaatcag tattaccagc ttatttcgtt
    14821 ctttcctgag ctaaaggatg agatctcttt tgtcgcggaa aacggcgcac tggtttacga
    14881 acatggcaag caactgttcc acggcgaact gacccgacat gaatcgcgga ttgttattgg
    14941 cgagttgcta aaagataagc aactcaattt tgtcgcctgc ggtctgcaaa gtgcatatgt
    15001 cagcgaaaat gcccccgaag catttgtcgc actgatggca aaacactacc atcgcctgaa
    15061 acctgtaaaa gattatcagg agattgacga cgtactgttc aagttttcgc tcaacctgcc
    15121 ggatgaacaa atcccgttag tgatcgacaa actacacata gcgctggatg gcattatgaa
    15181 acccgtcacc agtggttttg gctttatcga cctgattatt cccggtctac ataaagcaaa
    15241 cggtatttcg cggttactga aacgctggga tctgtcaccg caaaatgtgg tagcgattgg
    15301 cgacagcggt aacgatgcgg agatgctgaa aatggcgcgt tattcctttg cgatgggcaa
    15361 tgctgcggaa aacattaaac aaatcgcccg ttacgctacc gatgataata atcatgaagg
    15421 cgcgctgaat gtgattcagg ctgtgctgga taacacatcc ccttttaaca gctgacctct
    15481 ctaccggagc catgcgctcc ggttttccct tctttttaca tcctgtacat cacatcaaat
    15541 cattaaactg cattaactta tttataactt gtattgtcac atgcaaatca gcatagtttc
    15601 gaatgaaagt taatgcgagg tggttatggc ttctactttt accagcgaca cattgcctgc
    15661 cgatcacaaa gcagctatcc gtcagatgaa gcacgcgctg cgggcgcagc ttggcgatgt
    15721 ccagcagatc tttaatcagc taagcgatga cattgccacg cgagtggctg aaatcaacgc
    15781 actcaaagca cagggcgatg ccgtctggcc ggtgctgtct tatgccgata tcaaagcagg
    15841 tcatgttact gcagagcagc gcgaacagat taaacgtcgc ggttgtgcgg tgataaaagg
    15901 ccatttcccc cgcgaacaag cgctaggctg ggatcagtcg atgctggact atctgaaccg
    15961 caaccgcttt gacgaggtct acaaaggccc cggcgataat ttcttcggga cgctcagcgc
    16021 ttcacgtccc gagatttacc ccatctactg gtcgcaggcg caaatgcagg cccgccagag
    16081 tgaagaaatg gcgaatgcgc agtcgtttct caatcgtctg tggacatttg aaagtgatgg
    16141 aaagcaatgg tttaacccgg atgtgagcgt catctaccct gaccgtatcc gccgccttcc
    16201 gcccggaacg acctccaaag gtcttggagc gcataccgac tccggggcgc tggaacgctg
    16261 gctgcttcca gcgtatcagc acgttttcgc caacgtcttt aatggcaatc tggcgaaata
    16321 cgatccctgg catgcggcac atcgtacgga agttgaagag tacacggtgg acaacaccac
    16381 caaatgttcc gtgtttcgga cattccaggg ctggactgcg ctctcggata tgctgcctgg
    16441 tcaggggttg ctgcacgtcg tgcccattcc tgaagccatg gcgtacgtac tgttacgtcc
    16501 gctgcttgat gatgtgccgg aggatgaact gtgcggcgta gcgcccggaa gagtgttgcc
    16561 ggtatcagag caatggcatc cactgttaat tgaggcgtta accagcattc caaaactcga
    16621 ggccggagac tccgtctggt ggcactgcga cgtcatccat tccgttgccc ccgttgaaaa
    16681 tcaacagggt tggggcaacg tgatgtacat tcctgcggca ccgatgtgcg agaaaaatct
    16741 tgcctacgcg cacaaggtga aggccgcact ggaaaaaggc gcatcgccgg gcgacttccc
    16801 gcgcgaggac tatgaaacaa actgggaagg acgctttacg cttgccgacc tcaacattca
    16861 cggtaagcga gcgttgggca tggatgtttg agagttaatg gaagtgaatt gatgtgggat
    16921 taagaaaatc gattcgacgt tactcagatt gctgacaagt gcgctttctt aatgcctgat
    16981 gcggcgttaa cgccttatcc ggcctacaaa gttctgcaga ttcaatatat tacaggtact
    17041 gcgtaggcct gataagcgta gcgcatcagg ctattttgca tttgtcatca gccgtcgtca
    17101 ccccgacagt atcggggtga cggataatca cttactcgat ccctttacta cgcaggtaat
    17161 cttcgtaatt accgctaaag tcgatcacgc gttccggggt gatttccaga atgcgggtcg
    17221 ccagggagct tacgaactca cggtcgtgtg aaacaaagat cagcgtgccc tgatacagtt
    17281 ccagcgccat gttcagtgac tcgatggatt ccatatccag gtggttggtc ggttcgtcca
    17341 tgatcagaat gttcggcttc tgcatcatta acttaccaaa cagcatccgc cctttttccc
    17401 caccggaaag cactttagct ggctttttga tgtcgtcctg gctgaacagc aaacgaccga
    17461 gaatgctgcg taccgcctgc tcgtcatcgc cttcctgctt ccactgactc atccattcga
    17521 acacggtcag atcattttca aactcatatt cgtggtcctg ggcgtagtaa ccaatgcgcg
    17581 cgttttcaga ccattttacg gtgccgctgt ccggttgcag atcgcccacc agcgttttca
    17641 gcagcgttga tttaccgacg ccgttggtac ccagtaccgc aagtttttca ccgacctcca
    17701 gcagcaggtt gagattttta aacagtggac cgttatcaaa ccctttggtc agaccttcca
    17761 cttccagcgc gttacggaac agtttcttat cctgttcaaa acggatgaac gggttctgac
    17821 ggctggaggc tttcacctct tccagtttga ttttatcaat ctggcgcgcg cgcgaagttg
    17881 cctggcgaga tttcgaggcg ttggcgctaa agcggctaac gaaagattgc aactcagcaa
    17941 tctgcgcttt cttcttggcg ttatcggcca gcagacgttc acgcgcctgg gtcgccgccg
    18001 tcatgtactc atcgtagtta cccggataaa cgcgcagctc gccgtaatcc agatccgcca
    18061 tgtgggtaca gaccatgtta aggaagtgac ggtcgtgcga gatgatgatc atggtgctgt
    18121 cacgctcgtt cagcacctgt tccagccagc gaatggtgtc gatgtcgagg ttgttggtcg
    18181 gttcgtcgag caggagaata tccggctcag caaacagcgc ctgcgccaga agcacacgca
    18241 gcttccagcc aggagcaact tcactcatcg ggccgtagtg ctgttccact ggaattccca
    18301 cgccaagcaa cagttcaccg gcgcgagctt ccgcagagta accgtccatt tcgccgtatt
    18361 tcacttccag atcggccact ttatagccgt cttcttcact catttccggc aaagcataga
    18421 tgcggtcgcg ctcctgcttc acttcccaca actctttatg ccccatgatc accgtatcca
    18481 gcacagtgaa cccttcaaag gcaaactgat cctgacgcag cttaccaatg cgctcgttgg
    18541 gatcgaggga aacgttaccc agcgtcggct caaggtcgcc gccgagaatc ttcataaagg
    18601 tggatttacc actaccgttc gcgccaatca ggccgtaacg gttgccgccg ccgaatttga
    18661 cggaaatgtt ttcaaacaac ggcttactgc cgaactgcat ggtgacgtta ctggaaacta
    18721 acacaggcgt atcctgaaaa gagatatgac aaaccgcgca ttatgccata agtctgaaag
    18781 aagatcctgc tcctcgctta aggtctacac tcaagccaaa ggcaaaaaat gtgatttcgt
    18841 acacatctga tttcactgtg agctggaatg aacttataat gcgcttccaa tactctaata
    18901 ttctcaaccc aatggcctgc caggcacaaa atctcgctta acatgaatat gaaattgaaa
    18961 acattattcg cagcggcctt cgctgttgtc ggcttttgca gtaccgcctc tgcggtaact
    19021 tatcctctgc caaccgacgg gagtcgcctg gttggtcaga atcaggtgat caccattcct
    19081 gaaggtaaca ctcagccgct ggagtatttt gccgcggagt accagatggg actttccaat
    19141 atgatggaag cgaacccggg tgtggatacc ttcctgccga aaggcggtac tgtactgaac
    19201 attccgcagc agctgatcct gccggatacc gttcatgaag gcatcgtcat taacagtgca
    19261 gagatgcgtc tgtattacta tccgaaaggg accaacaccg ttatcgtgct gccgatcggc
    19321 attggtcagt taggcaaaga tacgcctatc aactggacca ccaaagttga gcataagaaa
    19381 gcaggcccga cctggacgcc gaccgccaaa atgcacgcag agtaccgcgc tgcgggcgaa
    19441 ccgctaccgg ctgtcgttcc tgcaggtccg gataacccga tgggtctgta tgcactctac
    19501 atcggtcgcc tgtatgctat ccatggcacc aacgccaact tcggtatcgg cctgcgtgta
    19561 agtcatggtt gtgtgcgtct acgtaacgaa gacatcaaat tcctgttcga gaaagtaccg
    19621 gtcggtaccc gcgtacagtt tattgatgag ccggtaaaag cgaccaccga gccagacggc
    19681 agccgttata ttgaagtcca taacccgctg tctaccaccg aagcccagtt tgaaggtcag
    19741 gaaatcgtgc caattaccct gacgaagagc gtgcagacag tgaccggtca gccagatgtt
    19801 gaccaggttg ttcttgatga agcgatcaaa aatcgttccg ggatgccggt tcgcctgaat
    19861 taatcgtcaa aacgtaaaac aaaggcggac tcataatccg ccttttttat ttgccagacc
    19921 ttaccaggcc gggatcataa ctaacaaaac gtatcccacc agcgccgccc acaacagcat
    19981 cggtattgaa tagagatgga aacgccacca gatgcggcga tcgttcgcca tacgtagcgc
    20041 aatcaaattt gccagcgatc ctggtaataa accaaagccc cccacgttta ccgcccatgc
    20101 cagtaataaa gacggaggta cataattgag cagcaaaatg gtactcggca cgttactgat
    20161 cacctgcgat aaaccgactg ccgttaacca taacccgggt tcagataaat gactcacgtt
    20221 acccaacacg ccttgcaacg ctggaagctg ggtcagtaaa tggacgtcga taaacatcgc
    20281 cataaacacc agcagcaacg tccagtccac actcaacacc acgcgacgcg ccagcagcac
    20341 aaagcccgcc gccacaatca ccagtcccca cagctcttgt ttgaactcca gcgccgtcag
    20401 aaaaacgata tacagcccca gacaattcca caccagccgc ggtttccact ccggtgtttg
    20461 cattcccgta tggtattgca gcgcctttcc agggaaacaa caccagcaca acagcaggag
    20521 tgtcagcatc attgcgccag ccagcggtgc catttgggca ataaatccgg caaacgaaag
    20581 accagaacgt ccccagataa gaatattttg cgggttgcca attggcgtca gtagcgaacc
    20641 agcgttgact gccagcgcct caaaaataat cagccgatta accgggatct cacacagtct
    20701 ttttagcgtg atagtcagcg gaacaacaat aaacagcgcg acatcgttgg tctgaaaggt
    20761 agaaagcagc gccgccgcca gcaccataaa catcgccagc cgacgctccg tagcaaagcg
    20821 gcgcaccatt ttgcgcccca gcacatcaaa ataaccgctt aactccacac ctttggtcag
    20881 cagcatcagg ccgcttaagg tgatgatggt gtgccagtcg atagcagcag gccaggattt
    20941 cggtgcaaag ggcacgaaaa agcttaatcc gataccaaca agaattaata actgaaaaaa
    21001 acgatcgcct tgcagcgtgc gtaaaaaagg caggctcatt tggcaccgtg tttctgggta
    21061 aacaaacgaa aagcgtcgag cgtctcttca ctaacatggt gctccatgcc ttccgcgtcg
    21121 cgacgggcga tttccggact gacgcccaac accaacaaga aattttcgac tatctgatgt
    21181 cgctcgcggc tttcctgcgc cagcttctct ccttctgccg ttaaaaacac gccacgccag
    21241 gggatcattt caatcagccc catggttgcc agccgcttaa gcattttagc caccgtcggt
    21301 tgcgaaactc ccagacgagc agccatgtcc acctgacgag cttcccccac ttccctgatc
    21361 aagtcagaaa tcagctcaac gtaatcatca ataagctcgc gccgatgcgc ctctctgacc
    21421 tggcggaacc cttcaacgtg ctcttccacg ttcactaact gcgtcacttt ttttgctgtt
    21481 ggcgtacctg cgcgacgact cattgtgctt cctcattatg gtgacgcata catagcgttc
    21541 tcatttcgtg cgcacattgt aaaccagagt tgcgaaggta caaaaaatta acgttttagc
    21601 aatagctata taatatagcc tgtgctatat ctgtatgtaa tgcaatcatc cctcaaggat
    21661 cgacgggatt agcaagtcag gaggtcttat gaatgagttc aagaggtgta tgcgcgtgtt
    21721 tagtcattct ccctttaaag tacggttaat gctgctctct atgttgtgcg atatggtcaa
    21781 caacaaaccg cagcaagata aaccttcaga taaatagcgg cgtcgcggta cgccgcttca
    21841 ctcctgcttt tatgcaggca taacgcgttt tggtctgaaa aaaaccactt tttgtcggat
    21901 ttgcaatccc cttcgcaaaa gatttgttcg tcagtagttg acctgaacgg cggctcgctc
    21961 tatcttcttg cagccctgcg tatattgcgg ctcgcggatg cggacccctt tcccctcttc
    22021 acgcactctt gcaggtattg acccttgacg ccagggtaag cacatggcgt ttgttaccat
    22081 agtggcatat gaatttaacc ctcaaagaat cgcttgttac ccgtagccgg gtatttagcc
    22141 cgtggactgc gttctacttt ttacagtcgc tattaattaa cctcggctta ggttacccct
    22201 tcagtttgct ctacaccgct gcgtttacgg ctattttgct tttgctatgg cgaacattgc
    22261 ctcgcgtaca aaaagttctg gtcggtgtca gttcgctggt ggcggcttgt tatttccctt
    22321 ttgctcaggc ctacggcgcg cctaatttca atacattgct ggcattgcac tccaccaata
    22381 tggaagagtc gaccgaaatc ctgacgattt ttccgtggta cagctacctg gtcggcttat
    22441 ttatttttgc gctcggcgta atagcaatca ggcgaaaaaa agagaatgaa aaagcgcgct
    22501 ggaatacctt cgacagcctg tgtctggtat tcagtgtggc gacatttttt gttgctcccg
    22561 tgcaaaacct ggcctggggt ggcgtattta aactgaaaga tactggctat ccggtatttc
    22621 gttttgctaa ggatgtcatc gtcaataata acgaggtgat tgaagagcaa gaacggatgg
    22681 caaaactttc cggaatgaaa gatacctgga cggtcactgc cgttaagccg aagtatcaga
    22741 cctatgtggt agtgatcggt gaaagcgcgc gtcgcgatgc cctcggtgcc tttggcggtc
    22801 actgggacaa taccccgttt gccagcagcg ttaacggttt gatatttgct gactacattg
    22861 ccgccagtgg ctccacgcag aaatcgcttg gcttaacgct caatcgcgta gtcgatggca
    22921 aaccacagtt tcaggataac tttgtcaccc tagcaaatcg cgcgggcttc cagacctggt
    22981 ggttttccaa ccagggccaa atcggcgaat acgatactgc tatcgccagc atcgccaaac
    23041 gtgcagatga agtgtacttc ctgaaagaag gtaattttga agcagataaa aacacgaaag
    23101 acgaagcgtt actggatatg accgctcaag tgctggcgca agagcactcg caaccgcagc
    23161 tgattgttct gcatctgatg ggctcgcatc cgcaggcctg cgacaggaca caaggcaaat
    23221 acaaaacctt tgtgcaatcg aaagaaacgt cgtgctatct ctataccatg acgcaaacgg
    23281 acgatttact gcgcaagctg tacgatcagt tacgcaacag cggcagcagc ttctctctgg
    23341 tttacttttc tgaccacggt ctggccttta aagagcgcgg caaagacgtg caataccttg
    23401 cccatgatga taaatatcag caaaatttcc aggtgccttt tatggtcatt tccagcggcg
    23461 ataaagcgca tcgggtgatt aaagcccgcc gctcagccaa tgacttctta ggctttttct
    23521 cgcagtggac gggaattaaa gcgaaggaaa taaatatcaa atacccgttt atatctgaga
    23581 agaaagccgg gccgatatac atcaccaact tccagttaca gaaggtggat tacaaccatc
    23641 tcggaaccga tattttcgac ccgaaacctt aaaaacaaaa atccgccccg agaggcggat
    23701 ttttatatca ccaaagtgat tagaagcggt aaccaacacc ggcaatccag gtgcctacgt
    23761 caacgctacg aatacggctc tgctcgtaag agaagtccag agcaacgttt tccatcgggt
    23821 tgaactgcag accagcaccg taggagaaac cgtagtcgct ggtgtcgtgt ttgtaggtcg
    23881 ggtattcagt ggtctggaat ttaccataac ccacacccac tacaccgtag atgcttgccc
    23941 agtcgttaat gcggtaagcc ggaccagcag tgatgccgta gtactggttt ttgttgtagt
    24001 caccagagct tgcagtacgg cttttctcgg tgtaagtgaa agaaccgatc acacccagcg
    24061 ggctgttgtc ttcttcatag cggtatttca ggtttaaacc gcccattttg ttcatttggc
    24121 cctgagcgtc gctctgtgcg taaccgccag ttacggtaga agtcgcagct acggaagtac
    24181 ctgcggtgaa agccagaact gcggccagtg ctgaaagaca tgcaattttt ttcataacca
    24241 cctcaaatgt gattcaaata agtcctaagt tttaaatata tcaaaaatta atgggaaact
    24301 cttcgcgatt tgtgatgtct aacgggccat ttcatgtaac agaacgtttc catacaccgc
    24361 tatccatcta aatttaaatc actttttcag agaactgcgt aagtattacg catgttttcc
    24421 ctgtcattca tccagattat tcctaatcac cagactaatg attccatcaa tcctggcgca
    24481 ttttagtcaa aacgggggaa aattttttca acaaatgctc aaccagcatt gggtatatcc
    24541 agtacactcc acgctttact taagtctaga tatttgtggg agaaaggatg cctggttcat
    24601 tacgtaaaat gccggtctgg ttaccaatag tcatattact cgttgccatg gcgtctattc
    24661 agggtggagc ctcgttagct aagtcacttt ttcctctggt gggcgcaccg ggtgtcactg
    24721 cgctgcgtct ggcattagga acgctgatcc tcatcgcgtt ctttaagcca tggcgactgc
    24781 gctttgccaa agagcaacgg ttaccgctgt tgttttacgg cgtttcgctg ggtgggatga
    24841 attatctttt ttatctttct attcagacag taccgctggg tattgcggtg gcgctggagt
    24901 tcaccggacc actggcggtg gcgctgttct cttctcgtcg cccggtagat ttcgtctggg
    24961 ttgtgctggc ggttcttggt ctgtggttcc tgctaccgct ggggcaagac gtttcccatg
    25021 tcgatttaac cggctgtgcg ctggcactgg gggccggggc ttgttgggct atttacattt
    25081 taagtgggca acgcgcagga gcggaacatg gccctgcgac ggtggcaatt ggttcgttga
    25141 ttgcagcgtt aattttcgtg ccaattggag cgcttcaggc tggtgaagca ctctggcact
    25201 ggtcggttat tccattgggt ctggctgtcg ctattctctc gaccgctctg ccttattcgc
    25261 tggaaatgat tgccctcacc cgtttgccaa cacggacatt tggtacgctg atgagcatgg
    25321 aaccggcgct ggctgccgtt tccgggatga ttttcctcgg agaaacactg acacccatac
    25381 agctgctggc gctcggcgct atcatcgccg cttcaatggg gtctacgctg acagtacgca
    25441 aagagagcaa aataaaagaa ttagacatta attaaattta catttctgta tggttatgca
    25501 taaccatgca gaatttctcg ctacttttcc tctacaccgt ctttatatat cgaattatgc
    25561 aaaagcatat ttattccgaa aattcctagc gagcagataa ataagaattg ttcttatcaa
    25621 tatatctaac tcattgaatc tttattagtt ttgtttttta cgcttgttac cactattagt
    25681 gtgataggaa cagccagaat agcggaacac atagccggtg ctatacttag tctcgttaat
    25741 tactgggaca taacatcaag aggatatgaa attatgagta ccgctaaatt agttaaatca
    25801 aaagcgacca atctgcttta tacccgcaac gatgtctccg acagcgagaa aaaagcaaca
    25861 gtagagttgc tgaatcgcca ggttatccag tttattgatc tttctttgat taccaaacaa
    25921 gcgcactgga acatgcgcgg cgctaacttc attgccgtac atgaaatgct ggatggcttc
    25981 cgcaccgcac tgatcgatca tctggatacc atggcagaac gtgccgtgca gctgggcggt
    26041 gtagctctgg ggaccactca agttatcaac agcaaaaccc cgctgaaaag ttacccgctg
    26101 gacatccaca acgttcagga tcacctgaaa gaactggctg accgttacgc aatcgtcgct
    26161 aatgacgtac gcaaagcgat tggcgaagcg aaagatgacg acaccgcaga tatcctgacc
    26221 gccgcgtctc gcgacctgga taaattcctg tggtttatcg agtctaacat cgaataaatc
    26281 catcgctgat ggtgcagaac tttagtaccc gataaaagcg gcttcctgac aggaggccgt
    26341 tttgttttgc agcccacctc aacgcactta tttagtgcat ccatctgcta tccccagctg
    26401 attaagtaaa ttttttgtat ccacatcatc acacaaccgt tacataaaga ttgttttttc
    26461 atcaggtttt acgctaaata atcactgtgt tgagtgcaca attttagcgc accagattgg
    26521 tgccccagaa tggtgcatct tcagggtatt gctctataaa tcgtgcatca cgtttttgcc
    26581 gcatctcgaa aaatcaatga gttgcaaaac tggcacgatt ttttcatata tgtgaatgtc
    26641 acgcagggga tcgtcccgtg gatagaaaaa aggaaatgct atgaagtctg tattaaaagt
    26701 ttcactggct gcactgaccc tggcttttgc ggtttcttct catgccgcgg ataaaaaatt
    26761 agttgtcgcg acggataccg ccttcgttcc gtttgaattt aaacagggcg ataaatatgt
    26821 gggctttgac gttgatctgt gggctgccat cgctaaagag ctgaagctgg attacgaact
    26881 gaagccgatg gatttcagtg ggatcattcc ggcactgcaa accaaaaacg tcgatctggc
    26941 gctggcgggc attaccatca ccgacgagcg taaaaaagcg atcgatttct ctgacggcta
    27001 ctacaaaagc ggcctgttag tgatggtgaa agctaacaat aacgatgtga aaagcgtgaa
    27061 agatctcgac gggaaagtgg ttgctgtgaa gagcggtact ggctccgttg attacgcgaa
    27121 agcaaacatc aaaactaaag atctgcgtca gttcccgaac atcgataacg cctatatgga
    27181 actgggcacc aaccgcgcag acgccgttct gcacgatacg ccaaacattc tgtacttcat
    27241 caaaaccgcc ggtaacggtc agttcaaagc ggtaggtgac tctctggaag cgcagcaata
    27301 cggtattgcg ttcccgaaag gtagcgacga gctgcgtgac aaagtcaacg gcgcgttgaa
    27361 aaccctgcgc gagaacggaa cttacaacga agtctacaaa aaatggttca gtactgaacc
    27421 gaaataataa cgctacacct gtaaagcgca ctggcagttc cctctccccc ctggggagag
    27481 gattagggtg aggggcgcaa acccgctccg gggccattaa ttaccctgaa tttgattatt
    27541 tacaccacgg taacaggaac aacatatgca gtttgactgg agtgccatct ggcctgccat
    27601 tccgcttctg attgaaggtg ccaaaatgac cctgtggatt tcggtcctcg gtctggctgg
    27661 cggtctggta atcggattgc tggcaggttt tgcacgcacc ttcggtggtt ggatagccaa
    27721 ccacgtcgcg ctggtcttta ttgaagtgat ccgcggcaca cctatcgtcg tccaggtgat
    27781 gtttatctat ttcgccctgc cgatggcgtt taacgactta cgcatcgacc catttactgc
    27841 ggcggtggtc accatcatga tcaactccgg cgcgtacatt gcggaaatca cgcgtggtgc
    27901 ggtgctgtct atccacaaag gttttcgtga agcaggactg gcgctcggtc tttcacgttg
    27961 ggaaaccatt cgctacgtca ttttaccgct ggcactgcgt cgtatgctgc cgccgctggg
    28021 taaccagtgg atcatcagca ttaaagacac ctcactgttt attgttatcg gcgtggcgga
    28081 actgacccgt caggggcaag aaattattgc cggtaacttc cgcgcccttg agatctggag
    28141 cgccgtggcg gtgttctatc tgattattac cctggtgctg agctttattc tgcgtcgtct
    28201 ggaaagaagg atgaaaatcc tgtgattgaa tttaaaaacg tctccaagca ctttggccca
    28261 acccaggtgc tgcacaatat cgatttgaac attgcccagg gcgaagtcgt ggtgattatc
    28321 gggccgtccg gttccggtaa atcgaccctg ctgcgctgca tcaacaaact ggaagaaatc
    28381 acctccggcg atctgattgt cgatggcctg aaggttaacg atccgaaagt tgacgagcgc
    28441 ctgattcgcc aggaagcagg tatggtgttc cagcagtttt acctcttccc gcatctgacg
    28501 gcgctgggaa acgtcatgtt tggtccgcta cgcgtgcgtg gcgcgaacaa agaagaggcg
    28561 gaaaaactgg cgcgtgagct gctggcgaaa gtcggtctgg cagaacgtgc acatcactac
    28621 ccttccgaac tttctggtgg tcaacagcag cgtgtggcga ttgcccgcgc gctggcggtg
    28681 aagccgaaaa tgatgctgtt tgatgaaccg acttccgctc ttgacccgga actgcgccat
    28741 gaagtgctga aggttatgca ggatctggct gaagaaggga tgacgatggt gatcgtgacc
    28801 cacgaaatcg gttttgccga gaaagtagct tcgcggctga tctttatcga caaaggccgg
    28861 attgcggaag atggcaatcc gcaggtgttg atcaagaacc cgccgagcca gcgcttgcag
    28921 gaatttttgc agcacgtctc ttaataagac acattgcctg atgcgctacg cttatcaggc
    28981 ctacaggata tctggcaact tattaaaatt gcatgaactt gtaggacgga taaggcgttc
    29041 acgccgcatc cggcaaaaaa gcccgcacgt tgtcagcaac cggcttaata tcccttcctc
    29101 cctttcaccc gaaagggagg cacaccagat tcctctcatt taaaatcgcc cctcccccag
    29161 catctatact tatctttttg ctctgttttc tcactggagg agtcatgcgg tggatcctgt
    29221 tcatcctctt ctgcctgctg ggcgcacctg cccacgcggt atccataccc ggcgttacaa
    29281 ccacaacgac aacggactca acgactgaac cggccccgga accggatatc gaacaaaaaa
    29341 agcggcctat ggcgcactgg cggatgtgct ggataacgac acctcgcgta aagagttgat
    29401 cgaccagttg cgtactgtcg ccgcgacgcc ccctgcggaa ccggtcccga agatcgtgcc
    29461 gccgacgctg gttgaagagc aaaccgtgct gcaaaaggtc accgaagtca gccgccatta
    29521 tggtgaagcc ctttccgccc gcttcgggca actttatcgc aatatcaccg gctccccgca
    29581 taagccattt aatccacaaa ccttcagcaa tgcgctgagc catttttcaa tgttagcggt
    29641 attagtgttt ggtttttact ggctgattcg cctgtgcgca ctgccgctat atcgcaaaat
    29701 gggtcagtgg gcgaggcaaa aaaatcgtga gcgcagcaac tggttgcagc ttccggcgat
    29761 gattattggg gcgtttatta tcgacctgct gttactggcc ctgacattat ttgtcggcca
    29821 ggtattaagc gacaacctga atgcaggtag tcgcaccatc gctttccaac aaagtttgtt
    29881 tctcaacgcc tttgccctga ttgaattttt caaagccgta ctacgcctga ttttttgccc
    29941 aaacgtggcg gagctgcgcc cgttcacgat tcaggacgag agcgcccgtt actggagtcg
    30001 ccgcctgagc tggttaagca gcctgattgg ctatggcctg attgtggccg tgccgattat
    30061 ctctaatcag gtgaatgtac agataggtgc gctggcgaac gtcatcatta tgctgtgcat
    30121 gaccgtctgg gcgttgtacc tgatcttccg aaataaaaaa gagattaccc agcatttgct
    30181 caactttgcg gagcattcgc tggctttttt cagcctgttt atccgcgcct ttgcgctggt
    30241 gtggcactgg ctggcaagcg cctattttat cgtgctgttt ttcttttcgt tgttcgatcc
    30301 gggcaacagt ctgaaattta tgatgggtgc aacggtgcgc agcctggcga ttattggtat
    30361 cgcagcgttt gtttccggta tgttttcccg ctggctggcg aaaaccatca ccctctcgcc
    30421 acatactcag cgtaactatc cggagctaca aaaacggctg aatggctggc tttcggcggc
    30481 gctgaaaacg gcgcgtattc tgaccgtctg cgtggcagta atgttgctgt tgagcgcatg
    30541 gggattgttc gatttctgga actggctgca aaacggcgcg gggcagaaaa ccgtagatat
    30601 cctgatccgc atcgcactca ttcttttctt ctcggcggtt ggctggacgg tgctcgccag
    30661 tttgatcgaa aaccggctgg cttcggatat tcatggccgc ccgctaccca gcgcccgtac
    30721 gcgtaccctg ctgacgctgt tccgtaacgc gctggcggtg attatcagta ccatcaccat
    30781 catgattgtg ttgtcggaaa tcggcgtcaa tatcgcgcca ttgctggcag gtgccggggc
    30841 attaggtctg gctatctcgt ttggttcgca aacgctggta aaagatatta tcaccggggt
    30901 atttattcag tttgaaaacg gcatgaacac tggagatttg gtgactatcg ggccgttgac
    30961 cggcacagtg gaacggatgt cgattcgctc cgtgggcgtg cgccaggata ccggggcgta
    31021 tcacatcatt ccgtggtctt cgataaccac ctttgctaac ttcgtccgcg gtattggttc
    31081 ggtagtggca aactacgatg ttgatcgcca tgaagatgct gataaagcca atcaggcact
    31141 gaaagatgcg gtaacggaat tgatggaaaa cgaagaaatt cgcgggctga ttattggtga
    31201 accgaacttt gccgggattg tcggcttaag caataccgcg tttacactgc gtgtttcgtt
    31261 caccacgctg ccactcaaac agtggacggt acgctttgcc ctcgacagcc aggtgaaaaa
    31321 acatttcgac ctggcgggcg ttcgcgcgcc agtgcagact tatcaggtgc tgcctgctcc
    31381 gggcgcgacc ccggctgaac cgctgccgcc gggggagcca acgctttaac gttggcgatt
    31441 aacaaaacgg cggcgctgct cgtcgttcat aaaggtccag gcaataaagc gactctgctt
    31501 ttgcccctgg gccatctctt tttttaccac cttcaccgcg cccacgtcgg tcagagcacg
    31561 atacaacggc ggtaagtttt caccacgaga taccagcgag gtaaaccaca tcacctgctt
    31621 cgcgaagcct ttgctctctt caatcatctt tttgataaag ttgacttcac cgccttcaca
    31681 ccacaactcc tgttgctgac caccaaagtt cagtgcatcg tctttgttca gccccaggtt
    31741 acggcgttta cgctcactac ctgcccgcgc cgcagcggcg gaatcgtgga atggcgggtt
    31801 acacaaggtc gcgtcgtatt gctcgttttt gtggatgatg ccgttaaaaa tcgccccact
    31861 ctctttttgc cgacgcagac gaatggcgcg gttaagcccc ggattagcac tgataatcgc
    31921 ctgcgcactg cttaacgcct ggctgctggt ttcactaccg gtaaaacgcc agccatattc
    31981 atgtacgcca atcagcggat aaatacagtt cgcaccaacg ccgatatcca gaatgctggc
    32041 attcgctgga attgttccgc ttgcttcagc cagtaaatcg gcaaggtgat gaatataatc
    32101 cgcccggccc ggtactggtg ggcagagaaa accgtcgggg atatcccagt tcgctacggc
    32161 gtaaaaatgg gccagcaacg ccttattgag cgccttcacc gccagcggat tggcaaagtc
    32221 tacgctttgc tcccctgcgg gtgtaagcgt gaggaattgc ctgagttcag gattgacctg
    32281 acaaagcgtg gcgagatcgt agcggctgtg atgacggttg cgcggatgca accccggttt
    32341 ctgggcggac atagcattct cctttaagca gcggcgtaag atacccgcat aacgcgacag
    32401 ggtaaatatc tggctgcgtt tatcattccc caaaacctta acatggctat ggtttatggc
    32461 aattcatcat aaaaatcagc attatgcatg tcattgattt gtattatttt gtaaaaatat
    32521 atcgttcaaa aaataagcat atctctgtca attttttgcg tcatttgcat cgcgcaggcg
    32581 acctacactt atcaggcact acccgttgta tttatgagga tgtcattatg aaaaagtgcc
    32641 tcacactact gattgccacc gtcctgagcg gaatctctct cacggcttat gccgcgcaac
    32701 cgatgagtaa cctggacagc ggtcagctac ggcccgccgg taccgtttcg gcaactggcg
    32761 catcaaacct aagcgatctg gaggataaac tggcagaaaa agcgcgcgaa caaggcgcga
    32821 aaggttatgt cattaattcc gcaggcggaa atgaccagat gttcggtact gcaaccatct
    32881 acaaataacc ccaccaacgt aactgcattc gcctctggat gcagttttcg cctgttttgt
    32941 aatcaaaaaa catcaaatat gaacgcaatg taaataaatg tatttctttt tcgcgcaatg
    33001 ggtgatagaa aatcgcgtca agtgataatg cttatcaaaa ttattatcac ttgcacaaac
    33061 actatcacgg gattaacagt ggcatcgcat ccgcagagat gctttctcgt ggcagtgaaa
    33121 atttcaacat ataagaaaaa gtcacctgca aaatggaaaa caatcgcaat ttccctgcca
    33181 gacaatttca ttcgctcacg ttctttgccg gtctttgtat tggtattacg cctgtggcac
    33241 aggcactcgc cgccgaaggg caagctaacg cggatgacac gctggttgtc gaagcatcaa
    33301 cgccttcgct ttatgcgcca caacaatctg ccgatccgaa attctcgcgt ccggtagcgg
    33361 atactacccg cacgatgacg gtgatttctg aacaagtgat taaagatcag ggcgcaacca
    33421 accttaccga cgcgctcaaa aacgtccccg gcg
//
LOCUS       DADMEQ010000033        33044 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.033, whole genome shotgun sequence.
ACCESSION   DADMEQ010000033 DADMEQ010000000
VERSION     DADMEQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33044
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.033"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            14..4591
                     /locus_tag="JNZ05_001610"
     CDS             14..4591
                     /locus_tag="JNZ05_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY8564883.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRSSVASG
                     TDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNT
                     IALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLA
                     LGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQ
                     LYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTK
                     GKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVEANTTNITNLTDS
                     VGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKTTNDAV
                     ATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTETTSKIT
                     NVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGEDALKW
                     DKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGGNASVN
                     TDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHGTNGDA
                     SVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYTIANAD
                     YDNVGDALNAIDTTPDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAISAASSD
                     AINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALDDNALL
                     WDKTANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQNTQII
                     NQLAGNTDATYIEENGAGINYVRTNDNDLAFNDASASGVGATAVGYNAVASGASSVAI
                     GQNSSSTVDTGIALGSSSVSSRVIAKGSRDTSVTENGLVIGYDTTDGELLGALSIGDD
                     GKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAM
                     GAKTVVNGNAGIGIGLNTLVLADAINGIAIGSNARANHANSIAMGNGSQTTRGAQTGY
                     TAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDERVAQNTQSI
                     TNLNNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIA
                     AADNSVALGTGSVAEEENTISVGSSTNQRRITNVAASVNATDAVNVSQLKSSEAGGVR
                     YDTKADGSIDYSNITLGGGNGSTTRISNVSAGVNNNDAVNYAQLKQSAQETKQYTDQR
                     MVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSM
                     VSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            4959..6614
                     /gene="lldP"
                     /locus_tag="JNZ05_001611"
     CDS             4959..6614
                     /gene="lldP"
                     /locus_tag="JNZ05_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HAY8564884.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDITLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            6614..7390
                     /gene="lldR"
                     /locus_tag="JNZ05_001612"
     CDS             6614..7390
                     /gene="lldR"
                     /locus_tag="JNZ05_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HAY8564885.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            7387..8577
                     /gene="lldD"
                     /locus_tag="JNZ05_001613"
     CDS             7387..8577
                     /gene="lldD"
                     /locus_tag="JNZ05_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HAY8564886.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            8773..9246
                     /gene="trmL"
                     /locus_tag="JNZ05_001614"
     CDS             8773..9246
                     /gene="trmL"
                     /locus_tag="JNZ05_001614"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAY8564887.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(9299..10120)
                     /gene="cysE"
                     /locus_tag="JNZ05_001615"
     CDS             complement(9299..10120)
                     /gene="cysE"
                     /locus_tag="JNZ05_001615"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAY8564888.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10200..11219)
                     /gene="gpsA"
                     /locus_tag="JNZ05_001616"
     CDS             complement(10200..11219)
                     /gene="gpsA"
                     /locus_tag="JNZ05_001616"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAY8564889.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11219..11686)
                     /gene="secB"
                     /locus_tag="JNZ05_001617"
     CDS             complement(11219..11686)
                     /gene="secB"
                     /locus_tag="JNZ05_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HAY8564890.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(11749..12000)
                     /gene="grxC"
                     /locus_tag="JNZ05_001618"
     CDS             complement(11749..12000)
                     /gene="grxC"
                     /locus_tag="JNZ05_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HAY8564891.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12142..12573)
                     /locus_tag="JNZ05_001619"
     CDS             complement(12142..12573)
                     /locus_tag="JNZ05_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAY8564892.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            12818..14362
                     /gene="gpmM"
                     /locus_tag="JNZ05_001620"
                     /gene_synonym="pgmI"
     CDS             12818..14362
                     /gene="gpmM"
                     /locus_tag="JNZ05_001620"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAY8564893.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPC
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            14372..15655
                     /gene="envC"
                     /locus_tag="JNZ05_001621"
     CDS             14372..15655
                     /gene="envC"
                     /locus_tag="JNZ05_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HAY8564894.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKEHAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQVLNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            15659..16618
                     /locus_tag="JNZ05_001622"
     CDS             15659..16618
                     /locus_tag="JNZ05_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HAY8564895.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQHEAAKFNASAN"
     gene            complement(16605..17639)
                     /gene="waaH"
                     /locus_tag="JNZ05_001623"
     CDS             complement(16605..17639)
                     /gene="waaH"
                     /locus_tag="JNZ05_001623"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HAY8564896.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(17878..18903)
                     /gene="tdh"
                     /locus_tag="JNZ05_001624"
     CDS             complement(17878..18903)
                     /gene="tdh"
                     /locus_tag="JNZ05_001624"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAY8564897.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(18913..20109)
                     /gene="kbl"
                     /locus_tag="JNZ05_001625"
     CDS             complement(18913..20109)
                     /gene="kbl"
                     /locus_tag="JNZ05_001625"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAY8564898.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEDRLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(20384..21241)
                     /gene="yibB"
                     /locus_tag="JNZ05_001626"
     CDS             complement(20384..21241)
                     /gene="yibB"
                     /locus_tag="JNZ05_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HAY8564899.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRNRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            21546..22478
                     /gene="rfaD"
                     /locus_tag="JNZ05_001627"
     CDS             21546..22478
                     /gene="rfaD"
                     /locus_tag="JNZ05_001627"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HAY8564900.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            22488..23534
                     /gene="rfaF"
                     /locus_tag="JNZ05_001628"
     CDS             22488..23534
                     /gene="rfaF"
                     /locus_tag="JNZ05_001628"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HAY8564901.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            23538..24530
                     /gene="rfaC"
                     /locus_tag="JNZ05_001629"
     CDS             23538..24530
                     /gene="rfaC"
                     /locus_tag="JNZ05_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HAY8564902.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLDYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            24527..25735
                     /gene="rfaL"
                     /locus_tag="JNZ05_001630"
     CDS             24527..25735
                     /gene="rfaL"
                     /locus_tag="JNZ05_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HAY8564903.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(25771..26913)
                     /locus_tag="JNZ05_001631"
     CDS             complement(25771..26913)
                     /locus_tag="JNZ05_001631"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HAY8564904.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(26922..27935)
                     /locus_tag="JNZ05_001632"
     CDS             complement(26922..27935)
                     /locus_tag="JNZ05_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HAY8564905.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(27960..28667)
                     /gene="rfaY"
                     /locus_tag="JNZ05_001633"
     CDS             complement(27960..28667)
                     /gene="rfaY"
                     /locus_tag="JNZ05_001633"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HAY8564906.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(28693..29700)
                     /gene="waaO"
                     /locus_tag="JNZ05_001634"
                     /gene_synonym="rfaI"
     CDS             complement(28693..29700)
                     /gene="waaO"
                     /locus_tag="JNZ05_001634"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HAY8564907.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(29743..30540)
                     /gene="rfaP"
                     /locus_tag="JNZ05_001635"
     CDS             complement(29743..30540)
                     /gene="rfaP"
                     /locus_tag="JNZ05_001635"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HAY8564908.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(30533..31657)
                     /locus_tag="JNZ05_001636"
     CDS             complement(30533..31657)
                     /locus_tag="JNZ05_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAY8564909.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(31654..32712)
                     /gene="rfaQ"
                     /locus_tag="JNZ05_001637"
     CDS             complement(31654..32712)
                     /gene="rfaQ"
                     /locus_tag="JNZ05_001637"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HAY8564910.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         8734 a         8083 c         7798 g         8429 t
ORIGIN      
        1 gcgagcatca ttaatgaaca aaatatttaa agttatctgg aaccctgcga cagggaatta
       61 tactgttacc agcgaaacgg caaaaagccg tggcaagaaa tctgggcgca gtaagctgtt
      121 aatttctgcg ctggttgcgg gtggaatgtt gtcgtcgttt ggggcattgg cgaatgccgg
      181 gaatgacaac ggtcagggtg ttgattacgg tagtggatca gctggcgacg gctgggttgc
      241 tataggcaaa ggggcgaaag caaatacttt tatgaacacc agtggttcca gtactgctgt
      301 gggttatgac gctatagctg aaggccaata tagctctgcc atcgggtcaa aaacccatgc
      361 gattggtggt gcatcaatgg cctttggggt tagtgcaata tcagaaggcg atagaagtat
      421 agcactgggt gcctcttcgt attcattggg ccaatactca atggccctgg ggcgtagcag
      481 tgtagctagc ggtactgaca gcctcgcatt tggcagacaa tcacttgcca gcgcagcgaa
      541 cgctattgcg ataggtgctg agaccgaagc cgctgaaaat gcaactgcta ttggcaataa
      601 tgcgaaggca aaagggacta atagcatggc aatggggttc ggaagccttg ccgataaagt
      661 caatactatc gcattaggaa atggcagcca ggctctggca gataatgcaa tcgccatagg
      721 ccagggcaac aaagctgatg gcgtggatgc catcgctctg ggtaatggta gccagtcgag
      781 aggcttaaac accattgcct taggcacagc cagtaatgca actggtgata agagtcttgc
      841 gcttggtagt aatagcagtg ccaacggtat taactctgtc gcgctgggcg cagattccat
      901 tgcggattta gacaataccg tctctgtcgg caatagttca ttaaaacgca agatcgttaa
      961 tgtgaaaaat ggcgcgatca agtctgacag ttacgatgcc attaatggtt cacagcttta
     1021 tgccattagc gactcggtag caaaaaggct tggaggaggg gctgcagtag atgttgatga
     1081 cggtactgtt acagcaccaa cctacaattt aaaaaatggt agcaaaaata acgtaggggc
     1141 tgcgctcgct gtacttgatg aaaacaccct gcaatgggac caaaccaaag gcaaatacag
     1201 cgctgctcat ggtactagta gcccaactgc cagcgtaatc accgatgttg cggatggcac
     1261 gatttcagcc tccagtaagg atgcggttaa cggttcccaa ctgaaagcta ccaatgacga
     1321 tgtcgaagcc aacaccacca atatcaccaa cctgacggat tccgttggtg accttcaggc
     1381 tgatgccctg ctctggaacg aaactaaaaa ggcattcagt gcagctcacg gccaggatac
     1441 caccagcaaa ataaccaacg ttaaagatgc cgacctgacg gctgacagca ctgatgctgt
     1501 taacggctct cagctgaaaa ccaccaacga tgctgtggcg acgaatacca ccaatatcgc
     1561 caataacact tccaatattg ccactaacac caccaacatc tctaacctga ctgagacggt
     1621 gactaatctt ggtgaggatg cgctgaaatg ggataaggac aatggtgtat tcacggcagc
     1681 tcatggcacc gagaccacca gcaaaataac caacgttaaa gatggcgacc tgacgactgg
     1741 cagcaccgat gccgttaacg gctctcagct gaaaaccacc aacgatgccg tggcgacgaa
     1801 taccaccaat atcgccacta acaccaccaa catctctaat ctgactgaga cggtgactaa
     1861 tcttggtgag gatgcgctga aatgggataa ggacaatggt gtcttcactg cagctcatgg
     1921 caacaatacc gccagcaaaa tcaccaatat cctggacggc acagtcactg caaccagttc
     1981 cgatgccatt aacggtagcc agctttatga cttaagcagc aatatcgcca cctacttcgg
     2041 cggcaatgct tctgtgaata ctgacggcgt gtttaccggt ccaacctaca aaatcggtga
     2101 aacaaattat tataacgtcg gcgatgcact ggctgcgatt aactcctcat ttagcacgtc
     2161 tctcggcgat gctctgcttt gggatgccac cgcaggtaaa ttcagtgcca aacacggtac
     2221 taatggtgac gcaagcgtga tcactgatgt cgcagatggt gaaatttcag actccagttc
     2281 tgacgcagta aacggctcac aactccacgg cgtgagcagt tatgttgttg atgcgctggg
     2341 gggtggtgcc gaagtcaatg cagacggcac catcactgcg ccgacgtaca ccattgctaa
     2401 tgctgattac gataatgtcg gtgatgccct gaatgctatc gataccactc ctgacgacgc
     2461 tctgctctgg gatgcggacg ccggtgaaaa tggtgcattt agcgccgctc acggaaaaga
     2521 taaaactgcc agtgtaatca ctaacgtcgc taacggtgca atctctgctg ccagcagcga
     2581 cgcgattaac ggctcacaac tctataccac caataagtac atcgctgatg cgctgggtgg
     2641 tgacgcagaa gtcaacgctg acggcaccat caccgcaccg acttacacca ttgcgaacgc
     2701 cgagtacaac aacgtcggtg acgccctgga tgcgcttgat gataacgccc tgctgtggga
     2761 taagactgcc aatggcggtg ctggagccta caatgccagc catgacggta aagccagcat
     2821 catcactaat gtcgctaatg gcagtattag tgaggacagt accgatgcag tgaacggttc
     2881 tcagttgaat gcgacgaata tgatgattga gcagaacacc caaattatca atcagctcgc
     2941 tggtaacacc gacgcaacct atatcgaaga aaatggcgct ggtatcaact acgttcgtac
     3001 taacgacaac gatttagcct ttaacgatgc aagcgcctct ggtgtcggcg ctacagctgt
     3061 aggttataac gctgtcgcgt ctggtgccag cagcgtagcc attggtcaga acagcagcag
     3121 caccgttgat accggtattg cgctgggtag cagctccgtt tccagccgtg tgattgccaa
     3181 aggttctcgt gacactagcg taacggaaaa tggcttggtg attggttatg acaccactga
     3241 cggcgaactg ctaggtgcat tgtcaattgg tgatgacggt aaataccgcc aaatcatcaa
     3301 cgtagctgat ggttcagaag cccatgacgc cgttacggtt cgccagttgc agaacgctat
     3361 tggagcggtc gccactacgc caaccaagta ctttcacgcc aactcaacgg cagaagactc
     3421 actggccgtt ggtgaagact cgctggcaat gggtgcgaaa actgtcgtta atggtaatgc
     3481 aggtattggt atcggtttga acactctggt tctggctgat gcgatcaacg gcattgctat
     3541 cggcagtaac gcacgcgcaa accatgcaaa cagtatcgca atgggtaacg gttctcagac
     3601 cacccgtggt gcacagactg gctacaccgc ctacaacatg gacgcaccgc agaactctgt
     3661 aggtgagttt tctgtcggta gtgaagacgg tcaacgtcag atcacaaacg tcgcagctgg
     3721 ttcagcggat accgatgcgg ttaacgtggg tcagttgaaa gtcactgatg agcgcgtagc
     3781 gcaaaatacc cagagcatta ctaacctgaa caatcaggtc actaatctgg atactcgcgt
     3841 tactaatatc gaaaacggta ttggcgacat tgtcaccacc ggtagcacca agtacttcaa
     3901 gaccaacacc gatggcgtag atgccaacgc ccagggtaaa gatagcgttg ctattggttc
     3961 tggttccatt gctgccgctg acaacagcgt cgcactgggt accggttccg ttgcagagga
     4021 agaaaataca atctctgtag gttcttctac taaccaacgc cgtatcacca acgtagctgc
     4081 cagtgttaat gccaccgatg cggttaacgt gtcgcagctg aaatcttctg aagcaggcgg
     4141 agtgcgctac gacaccaaag ctgatggttc tatcgactat agcaatatca ccctcggtgg
     4201 tggcaacggc agtacgactc gtatcagcaa cgtctccgct ggcgtcaaca acaacgacgc
     4261 ggtgaactac gcgcagttga agcaaagcgc gcaggaaacg aagcaataca ccgatcagcg
     4321 gatggttgag atggataaca aactgtctaa aactgaaagc aagttgagcg gtggtatcgc
     4381 ttctgcaatg gcaatgaccg gtctgccgca ggcttataca ccgggtgcca gcatggcttc
     4441 tattggtggc ggtacttaca acggtgaatc ggcagttgct ttaggtgtat cgatggtgag
     4501 cgccaatggt cgttgggtct acaaattaca aggtagtacc aatagccagg gtgaatactc
     4561 cgccgcactc ggtgccggta ttcagtggta atgatccatt aagttagtgt gactaagggc
     4621 aaacgccaaa ttgcctgatg cgttacgcgc atcaggcaag ataaggcgct ccgggtcaac
     4681 aatctaaggg caattctctg atgaggattg cccttttctt taccagacat ctccccccac
     4741 aagaattggc cctaccaatt cttcgcttat ctgacctctg gttcacaatt ccccaattaa
     4801 aactcacatc aatattgcta atacataaca tttagttaac cattcattgt cattatccct
     4861 acacaacaca attggcagtg ccacttttac acaacgtgtg acaaggagat gagcaacaga
     4921 ctcattacac gatgtgcgtg gactctagga gacctgcaat gaatctctgg caacaaaact
     4981 acgatcccgc cgggaatatc tggctttcca gtctgatagc atcgcttccc atcctgtttt
     5041 tcttctttgc gctgattaag ctcaaactga aaggatacgt cgccgcctca tggacggtag
     5101 caatcgccct tgccgtggct ttgctgttct ataaaatgcc ggtcgctaac gcgctggcct
     5161 cggtggttta tggcttcttc tatggtttgt ggcccatcgc gtggatcatt attgcagcag
     5221 tgttcgtcta taagatctcg gtgaaaaccg ggcagtttga catcattcgc tcgtctattc
     5281 tttcgataac ccctgaccag cgtctgcaaa tgctgatcgt cggtttctgt ttcggcgctt
     5341 tccttgaagg agccgcaggc tttggcgcac cggtagcaat taccgccgca ttgctggtcg
     5401 gcctgggttt taaaccgctg tacgccgccg gactgtgcct gattgttaac accgcgccag
     5461 tggcatttgg cgcgatgggc attccaatcc tggttgccgg acaagtaaca ggcatcgaca
     5521 gctttgagat tggccagatg gtggggcgtc agctaccgtt tatgaccatt atcgtgctgt
     5581 tctggatcat ggcgattatg gacggctggc gcggtatcaa agagacgtgg cctgcggtcg
     5641 tggttgcagg cggctcgttt gccatcgctc agtaccttag ctctaacttc attgggccgg
     5701 agctgccgga cattatctct tcgctggtat cactgctctg cctgacgctg ttcctcaaac
     5761 gctggcaacc agttcgcgta ttccgcttcg gtgacttggg ggcgtcacag gttgatataa
     5821 cgctggccca caccggttac accgcgggtc aggtgttacg tgcctggaca ccgttcctgt
     5881 tcctgaccgc taccgtaaca ctgtggagca tcccgccgtt taaagccctg ttcgcatcgg
     5941 gtggtgcgct gtatgagtgg gtgatcaaca ttccggtgcc gtacctcaat aaactggttg
     6001 cccgtatgcc accagtggtc agcgaggcta cagcctatgc cgccgtgttt aagtttgact
     6061 ggttctctgc caccggcacc gccattctgt ttgctgcact gctctcgatt gtctggctga
     6121 agatgaaacc gtctgacgct atcagcacct tcggcagcac gctgaaagaa ctggctctac
     6181 ccatctactc catcggcatg gtgctggcgt tcgcctttat ctcgaactat tccggactgt
     6241 catcaacact ggcactggca ctggcgcaca ccggtcatgc attcaccttc ttctcgccgt
     6301 tcctcggctg gctaggggta ttcctgaccg ggtcggatac ctcatctaac gccctgttcg
     6361 ccgcgctgca agccaccgca gcacaacaaa ttggcgtctc tgatctgttg ctggttgccg
     6421 ccaataccac cggtggcgtc accggtaaga tgatctcccc gcaatctatc gctatcgcct
     6481 gtgcggcggt aggcctggtg ggcaaagagt ctgatttgtt ccgctttact gtcaaacaca
     6541 gcctgatctt cacctgtatg gtgggcgtga tcaccacgct tcaggcttat gtcttaacgt
     6601 ggatgattcc ttaatgattg ttttacccag acgcctgtca gacgaggttg ccgatcgtgt
     6661 gcgggcgctg attgatgaaa aaaacctgga agcgggcatg aagttgcccg ctgagcgcca
     6721 actggcgatg caactcggcg tatcacgtaa ttcactacgc gaggcgctgg caaaactggt
     6781 gagcgaaggc gtgctgctca gtcgacgcgg cggcgggacg tttattcgct ggcgtcatga
     6841 cacatggtcg gagcaaaaca tcgtccagcc gctaaaaacg ctgatggccg atgatccgga
     6901 ttacagtttc gatattctgg aagcccgcta cgccattgaa gccagcaccg catggcatgc
     6961 ggcaatgcgc gccacacctg gcgacaaaga aaagattcag ctttgctttg aagcaacgct
     7021 aagtgaagac ccggatatcg cctcacaagc ggacgttcgt tttcatctgg cgattgccga
     7081 agcctcacat aacatcgtgc tgctgcaaac catgcgcggt ttcttcgatg tcctgcaatc
     7141 ctcagtgaag catagccgtc agcggatgta tctggtgcca ccggtttttt cacaactgac
     7201 cgaacaacat caggctgtca ttgacgccat ttttgctggt gatgccgacg gggcgcgtaa
     7261 agcaatgatg gcgcacctta gttttgttca caccaccatg aaacgattcg atgaagatca
     7321 ggctcgccac gcacggatta cccgcctgcc cggtgagcat aatgagcatt cgagggagaa
     7381 aaacgcatga ttatttccgc agccagcgat tatcgcgccg cagcgcaacg cattctgccg
     7441 ccgttcctgt tccactatat ggatggtggt gcatattctg aatacacgct gcgccgcaac
     7501 gtggaagatt tgtcagaagt ggcactgcgc cagcgtattc tgaaaaacat gtccgactta
     7561 agcctggaaa cgacgctgtt taatgagaaa ttgtcgatgc cggtagcact ggcaccggtg
     7621 ggtttgtgtg gcatgtatgc gcgtcgtggc gaagttcagg cagccaaagc ggcggacgcg
     7681 catggcattc cgtttactct ctcgacggtt tccgtttgcc cgattgaaga agtcgcgcca
     7741 gccatcaagc gcccaatgtg gttccagctt tatgtactgc gcgatcgcgg ctttatgcgt
     7801 aacgcgctgg agcgagcaaa agcagcgggt tgttcgacgc tggttttcac cgtggatatg
     7861 ccgacaccgg gcgcacgcta ccgtgatgcg cattcaggta tgagcggccc aaatgcggca
     7921 atgcgccgct acttgcaggc ggtgacgcat ccgcaatggg cgtgggatgt gggcctgaac
     7981 ggtcgtccac atgatttagg taatatctca gcttatctcg gcaaaccgac cggactggaa
     8041 gattacatcg gctggctggg gaataacttc gatccgtcca tctcatggaa agaccttgag
     8101 tggatccgcg atttctggga tggcccgatg gtgatcaaag ggatcctcga tccggaagat
     8161 gcgcgcgatg cagtacgttt tggtgctgat ggaattgtgg tttctaacca cggtggccgc
     8221 cagctggacg gtgtactctc ttccgctcgt gcgttgcccg ctattgcaga tgcggtgaaa
     8281 ggtgatatcg ccattctggc ggatagcgga attcgtaacg ggcttgatgt cgtgcgtatg
     8341 attgcgctcg gtgccgacac cgtactgctg ggtcgtgctt tcctgtatgc gctggcaaca
     8401 gcgggccagg cgggtgtagc taacctgcta aatctgatcg aaaaagagat gaaagtggcg
     8461 atgacgctga ctggcgcgaa atcgatcagt gaaattacgc aagattcgct ggtgcagggg
     8521 ctgggtaaag agttgcctac ggcactggct ccgatggcga aagggaatgc ggcatagtcg
     8581 tttgcgcccc ctcaccctaa ccctctccct cagggagagg ggaccgttcg gcgctgtatg
     8641 tactccctca ctctgaaacg acaccgcact ctttttttct ccctcgcccc tccggggagg
     8701 gccggggtga ggggaaaagg ccgcacggtt caccaatttt ctgctatcct gcccccgcac
     8761 taagggggca gtatgctaaa catcgtactt tacgaaccag aaattccgcc aaatactggc
     8821 aacatcatcc gtctttgcgc taataccggc tttcgtctac atatcatcga accgatggga
     8881 tttgcctggg acgacaagcg cctgcgccgc gcggggctgg actatcacga gtttaccgcc
     8941 gttacgcgtc atcatgacta tcgcgcgttc ctcgaagcag aaaatcccca gcgcctgttt
     9001 gccctcacca cgaaaggtac gcctgctcac agcgccgtaa gctatcagga tggcgattat
     9061 ctgatgttcg gcccggaaac acgcggcctg ccagcgagca ttcttgatgc cctgcccgct
     9121 gaacaaaaaa ttcgcattcc gatggtgccg gacagccgca gcatgaatct gtccaatgcg
     9181 gtgtcggtag tggtgtatga agcctggcgg cagctgggat atccgggagc gttgttgaga
     9241 gattagttac tgtatgccgg atagatgatt acatcgcatc cggcacgatc acaggacatt
     9301 agatcccatc cccatactca aatgtatggt taataccgtt gaaatgctgg tccatatcca
     9361 ttgatggctt atcgctgtct ggtttaccga caatacgagc cggaacgcca gcggcggttg
     9421 tatgcggcgg caccggttgc agcaccacgg aacctgcgcc aatcttcgcg ccgcgaccaa
     9481 cttcaatatt gccgaggatt ttcgcgcccg cgccaatcat cacaccttca cgaattttcg
     9541 ggtgacggtc accaccagat ttacccgtac cgccaagcgt cacagattgc agaatcgata
     9601 cgtcgttttc aattaccgcc gtttcaccca caacgatgcc cgtggcgtgg tcgagcataa
     9661 tgccacggcc aatttttgct gccgggtgaa tatcgacctg gaacgtcaca gaaacctggt
     9721 tttgcagaaa gattgccaat gcgcgacgcc cctgattcca caaccagtga ccgatgcgat
     9781 aggcctgcaa ggcatgaaaa cccttcaggt ataacaacgg ggttgagtat ttatcgactg
     9841 ccgggtcgcg ggtacgcacc gcctgaatat cacaggccgc agaggcgatc atttccgggt
     9901 cagcggcgta ggcttcttca accacttcac ggatagcaat agcaggcata attggcgatg
     9961 acagcttgtt cgccagcatg tagctcagtg cactgccaag gttttcgtgc ttgagtagcg
    10021 tcgcgtggta aaaactggcc agcattggct cacagtccgc cagcgttctg gcttcggctt
    10081 taatattgtt ccagacaatt tccagttctt cacacgacat tgcttactcc acacgatgag
    10141 ataatgaccg gcccgttctg cgcgggccgg gtcatagcgg taacaaaggt tccctggggt
    10201 tagtggctgc tgcgctcgtc cttgcgtgca cgacctaata atgtcaatgc tgcctcgcgc
    10261 gcgttttttc cgcaatataa tacttgataa atttcctcgg ttattggcat ttcaacgccg
    10321 aagcgatgcg ccagttcgcg gacttctttc gtattgcggt agccttccac cacctgacca
    10381 atcttctcct gcgcgctttg tacatccatg ccctgaccga gcatcatgcc aaaacggcgg
    10441 ttacgcgact ggttgtcggt acaggtaagc accagatcgc ccagccccgc catgcccata
    10501 aaggtggcag gatcggcacc cagcgccgca ccaagacgcg acatttcagc cagcccacgg
    10561 gtgatcagcg ccgtacgcgc attcgcacca aaaccgatac cgtcagacat ccccgcgcca
    10621 atggcaatga cgtttttcac cgcgccgcca agctgcacgc caatgaaatc cgggttgctg
    10681 taaacgcgga aacttttgcc gcagtgcagc agctgctgga gatcatcggc aaaggtctga
    10741 tcggtcgagg ccagcgaaat agctgtcggt aaacctgccg ccagttcttt cgcaaacgtt
    10801 gggccagaga taaccgccag cggaatttga tcgcctaagg cctcacgcgc cacgtcctgt
    10861 aacagacgtc cggtttccgc ttccagccct ttggtcgccc acaccagacg cgcatcagga
    10921 cgcatcagcg gtttaatctg gcgcagcact tcaccaaaga catggctggg tacgacgacg
    10981 agaatattac gactggctgc cagcgcagtg gcgagatcgc tttcaagatg gagcgtatcg
    11041 ggaaaaggca catcggggag aaacgcggcg ttacagcggt cgcgttcaag cgttgcgata
    11101 tgttcagggt catggcccca gaggacaacc tcgtggccat ttcttgccag ggtaatggca
    11161 agagcggtgc cgtacgagcc ggcaccgatc acagtcattg aagcattacg ttggttcatc
    11221 aggcatcctg atgttcttca gtaccttcgc cagcctgctg ctgcaaatag ttcatgaaca
    11281 gcgcatcgaa gttaaccggc gcaaggttca gttgcgggaa tgtaccgcgg gataccatgc
    11341 tggtgatgca ctcacgagca tacgggaaca gaatgttcgg gcagtatgcg cccaggcaat
    11401 gtgccatctg ggtgccttcg atacccgcga tggagaaaat accgccctgc tgaacttcac
    11461 acaggaacgc ggtttcttcg cccaaggagg ccgttacggt aacacgcagt accacttcgt
    11521 atacgtcatc tgccagttgg gaagatgccg tatccagatc aagtttaact tctggttgcc
    11581 aatctttctg gaaaacgtgc ggcgcgttcg gcgcttcgaa agagatatcc ttggtataaa
    11641 tacgttggat ctggaaagtc atttcagtgt tgttttgttc tgacatgtgt agaaaaccct
    11701 taagtgttgt ccttaaatac tgcgcaatgt aatgccaacg ttcacacgtt atttcagcag
    11761 gggatccagt ccaccacgtg catccaatgc atacaagtca tcacagccgc caatgtgctg
    11821 tgcgtcaata aaaatctggg gaaccgtggt gcgaccgctg cgtttgatca tctcttcacg
    11881 cttggcggca ttgccatcga tcggcaactc ctggaaactc acgcccttgc tgctcagcag
    11941 tgcttttgca cgatggcaat acgggcaggt ttctttggta tagatttcaa cattggccat
    12001 aatttagctc ctgttttttt accctgaaat ttcatgttgc agggcggctg caaaatggag
    12061 tgtccccaga agcttataac aataagtcgc tgggggccta attgcagcta acggcctgca
    12121 tcatgaaaga cgacaggtaa attatttgcc gcgcaccaga ggcaagtttt cgccagccca
    12181 gccagcaacg ccctctttca gcacgaatac ttgcgcaaaa ccagctttcg tcagcgcgtt
    12241 tgcaggctcc tggcactgca tgccagaacc gtctaccacg ataaccgatt tgtctttgtg
    12301 cttctcaagc tcaccaacat tgttggcttt gatttcgctc ggcaacagat tgatagaacc
    12361 tgcgatatgg cctttacgga agtcatcacg ctgacgtaaa tccacaacca cagcgtcttc
    12421 tttattgatc agacgcgtag cttcaccacg agtaatcacc ttcactttcg aggtcaggct
    12481 cttaaacgta gtcacaagaa ccgccaccag taacgcgatc caggcgatac tcagtatggg
    12541 atgacggcca acaaattgca taatttcttg catggggggt aacaactccc aacgtagtga
    12601 ttaaaaaaac caggaaagga gtatacctgc gcggtgcggc aaatacagcc agcgcgttaa
    12661 ctggaatgca attttgcggg gcacgacgaa aaaaaagcgc aaatctggca tcggcttgcc
    12721 gcgagcggtc gtatattttg atcttcagag gctattttat cgattcagct gtagtaaaat
    12781 tacgcaaatt ttgactcttg agtatgaggt tgtcgcaatg tcggtttcta aaaaacctat
    12841 ggtactggtg attctggatg gctatggcta tcgtgaagaa cagcaggata acgccatttt
    12901 tagtgctaaa accccggtaa tggatgcact gtgggccaat cgtccgcata ccctgatcga
    12961 cgcttccggt ctggaagtcg gtctgcctga ccgtcagatg ggtaactccg aagtaggcca
    13021 cgttaacctg ggtgccggcc gcatcgtgta tcaggacctg actcgtctgg acgttgaaat
    13081 caaagatcgc gctttctttg ctaatccagt gctggctggc gcggtacata aagcgaaaaa
    13141 cgcaggtaaa gcagtacaca ttatgggtct gctctccgca ggcggcgtac acagccacga
    13201 agatcacatc atggcgatgg tagaactggc agctgaacgc ggcgcagaaa aaatctacct
    13261 gcacgcattc cttgacggtc gcgacacccc gccgtgcagt gctgaatcct cgctgaaaaa
    13321 atttgaagag aaatttgctg cgctgggtaa aggccgcgta gcgtccatca ttggtcgtta
    13381 ctacgcgatg gaccgcgata accgttggga tcgcgttgaa aaagcctatg acctgctgac
    13441 tctggcgcag ggtgagttcc aggccgatac tgccgttgct ggtttgcagg cagcttatgc
    13501 tcgcgacgaa aacgacgagt tcgtgaaagc gaccgttatt cgtgcagaag gtcagccaga
    13561 tgcggcaatg gaagacggcg atgcgctgat tttcatgaac ttccgtgctg accgcgcgcg
    13621 tgaaatcact cgtgcattcg tgaacgctga tttcgatggc ttcgcgcgta agaaagtggt
    13681 taacgtcgat ttcgtgatgc tgaccgaata cgccgctgac atcaaaactg cggttgctta
    13741 cccacccgct tccctggtta acaccttcgg cgagtggatg gcgaaaaacg acaaaactca
    13801 gttgcgtatt tccgaaaccg aaaaatatgc ccacgttact ttcttcttca acggtggcgt
    13861 agaagagtcg ttcaaaggcg aagatcgcat tctgatcaac tcaccgaaag tggctaccta
    13921 cgatctgcaa ccggaaatga gctccgcaga gctgaccgaa aaactggttg cggccatcaa
    13981 gagcggcaaa tacgacacca ttatctgtaa ctatccgaac ggcgacatgg taggtcacac
    14041 cggggtgatg gaagcggcgg ttaaagcggt tgaagcactg gatcactgcg tggaagaagt
    14101 cgcgaaagcg gttgagtccg tgggtggaca actgctgatc accgctgacc acggtaacgc
    14161 tgaacagatg cgcgatccgg caacgggtca ggcacacacg gcacacacca acctgccagt
    14221 tccgctgatt tacgttggtg ataagaacgt gaaagcggtt gcaggcggca aactttctga
    14281 catcgcgccg accatgttgt cgctgatggg tatggaaatc ccgcaagaga tgactggtaa
    14341 gccgctgttc atcgtggaat aatccctccc catgagggga aaggcgatta ataccatgac
    14401 acgggccgtg aaaccgcgca ggtttgcaat caggcccatc atctacgcca gcgttcttag
    14461 cgctggcgta ttgttgtgcg ccttttccgc ccacgcggat gagcgtgacc aactcaaatc
    14521 tattcaggcc gatatcgccg caaaagagca cgcggtacgc caaaagcaac aacaacgcgc
    14581 aagcctgctc gcacaattga aaaagcagga agaagcgatc tctgaagcca cccgtaagct
    14641 gcgcgaaacg caaaacacgc tcaatcaact caataaacag attgatgaga tgaacgcgtc
    14701 gattgccaaa ctggagcagc aaaaagccgc ccaggagcgc agcctcgccg cacaactgga
    14761 tgccgcattc cgtcagggcg agcataccgg tattcagcta attctcagcg gtgaagaaag
    14821 ccagcgtgga cagcgtttac aggcttattt cggctatctc aaccaggcgc gacaagaaac
    14881 cattgcccag ttgaagcaaa cgcgtgaaga agtcaccatg cagcgtgctg aactggaaga
    14941 gaaacaaagc gagcaacaaa cgctgttata tgaacagcgc gcccaacagg cgaaactgac
    15001 tcaggttctg aacgagcgta aaaagacgct ggcggggctg gaatcttcca tccagcaagg
    15061 tcagcagcag ctaagtgaac tgcgtgccaa cgaatcccgt ctgcgtaaca gcattgcccg
    15121 tgcggaagcc gcggcgaaag cgcgtgctga acgagaagcg cgtgaggccc aggcggttcg
    15181 cgagcgccag aaagaagcga cgcgcaaagg caccacctac aaaccgaccg aaagcgaaaa
    15241 atcgctgatg tcccgaactg gtggcctggg tgcgccgcgt ggtcaggcat tctggccggt
    15301 tcgcgggccg acgctgcatc gctatggcga gcagctacag ggtgaattac gctggaaagg
    15361 gatggttatc ggtgcctctg aaggtactga agttaaagca attgccgacg gtcgagtgat
    15421 tctggctgac tggctgcaag gttacggtct ggtggtggtg gttgagcacg gtaaaggcga
    15481 catgagtctt tacggctata atcagagcgc actggtgagc gttggttcgc aggttcgcgc
    15541 gggccagcca attgcactgg tgggcagcag tggcggtcag ggtcggcctt cactctattt
    15601 cgaaattcgc cgccagggtc aggcggtcaa tccacagccg tggttgggaa gataagtttt
    15661 gtttccattt cgtcgtaacg ttcttgcatt tgccgctctg ttggcgctct cctcccccgt
    15721 acttgctggc aaacttgcca tcgtcattga tgattttggg tatcgcccgc acaacgaaaa
    15781 ccaagtgctg gcgatgcctt ccgctatctc cgtcgctgta ttacccgatt caccgcacgc
    15841 cagagagatg gcgaccaaag cgcataacag cggtcacgaa gtgttgattc atctcccgat
    15901 ggcaccgttg agtaaacagc cgctggagaa aaatacgcta cgcccggaga tgagcagcga
    15961 cgaaattgag cgcattattc gtagtgcggt caataacgtg ccctatgccg tggggatcaa
    16021 caaccacatg ggcagcaaga tgacctctaa cctgtttggt atgcagaaag tgatgcaggc
    16081 gctggagcgt tacaatcttt acttcctcga cagcgtaacc atcggtaata cccaggcgat
    16141 gcgcgccgcg caaggtactg gcgtgaaggt gatcaaacgg aaggtgttcc tcgacgattc
    16201 gcagaacgaa gcggacatcc gtgtgcaatt taatcgcgca attgatctgg cgcgtcgcaa
    16261 cggttcaacc attgccattg ggcatcctca cccttcaacg gtacgcgtgc tacaacagat
    16321 ggtttataac ctgccgccag acattacgct ggtgaaagcc agcagcttgc tgaatgaacc
    16381 gcaggttgat acttctacac cgccgaaaaa cgctgtgcct gatgcaccgc gtaatccgtt
    16441 ccgtggcgtg aagctgtgca aaccgaagaa accgatagaa cctgtttatg ctaatcgctt
    16501 ctttgaagta ttaagcgaaa gcatcagcca gagcacgctg atcgtttact tccagcatca
    16561 gtggcaaggc tggggcaaac agcacgaagc ggcgaagttt aacgctagcg caaattaaag
    16621 gcgctacgcg taatgcgatg gtgcgaccgc gttttgtcgc gccattgcca taagcggaaa
    16681 gaccacagta acgcctggta accgaccttc acgctgcgca cattggtaat caggcgctta
    16741 tacataccgg aagtaaatat ctcggcaatc atccgttggc gggtaagaat atccggctct
    16801 ttgcgcaccg catggcaaac acgcaatgct tcgtaagtta tttgctgatg aaattccgga
    16861 taaatcgtaa ttttgtcggc ataatttcga tttaatttct ccagcaggcg ggtaatctta
    16921 atatagtgac gttgataatt aaggttttta ttcccttgtc tatgcaaccg actcactgac
    16981 gtattatgca gataatattt atataatgat tgctcggtat atcgcgcacg cagcgcgtta
    17041 aacatgaatt ctgttgtcca gacaatatcc tgatgatgta atccggcaat aaatttaatg
    17101 ttatttttaa caataacatc acggcgataa acccccatcc agacaacgtg agtccaacgg
    17161 cgcgaagaaa gccccatccg cagccagtcc gggccggtta atacaccggt tgagcgaagg
    17221 cgatcgctgg ggatggattg ccaggtttct cccgtttcac gaaaagacca gtcagcgttg
    17281 cactgcgcca cgtcgaggtc gtcctctaac gccatggtca tcagcgtttc gtacatggtg
    17341 ggatagacct catcgtcagc atcgacaaaa gcgacatatt tgcccgtcgc cacctcgatc
    17401 ccacgattac gcgctaccga tgcgccagca ttcgcctgat gcaacaaacg aacgtgcgga
    17461 tagttttctg cgtaatgctt tgctatttca acagaattat ccgttgaacc atcgttaata
    17521 ataatgattt ccagagcagt ccaggtttgc gtaattaaag attccataca agtgcggaaa
    17581 tcatcgcccg cattatataa cggaataata acactaagtt tattggtgct gttcatcata
    17641 aattaccgac gacctctgaa gttatcctga cgttttacag gctttgtatt aagaaactat
    17701 taagcctgtg agatattatt tttacaggtg agagagtcac ttcagggtag agagataatg
    17761 agaacagcta tataaaagat cagcgcaatt acgaactctt tattacactg acaggatttt
    17821 acataattaa cagattatcc tcagataaaa gggctggaat accagccctt gttcgtgtta
    17881 atcccagctc agaataactt tcccggactg gcccgaacgc atagcgtcaa agcccttctg
    17941 gaaatcatcg atagagaaac gatgggtaat gatcggcgag agatcgaggc cagactgaat
    18001 cagcgccgcc atcttgtacc aagtttcaaa catctcacga ccgtaaatgc ctttaatgaa
    18061 caagccttta aagatcactt tggtccagtc gatagacata tcagacggtg gaatacccag
    18121 catcgcaata cgaccgccgt ggttcatggt atcgagcatg gtacgaaacg ctggcggcgc
    18181 gccggacatt tccagaccga catcaaagcc ttcggtcatg cccagttcag ccatcacatc
    18241 attaaggttt tctttcgcaa cgttaaccgc acgggtgata cccattttgc gcgccagctc
    18301 aaggcggtat tcgttaacat cggtgatcac cacattgcgt gcaccaacgt gtttcgccac
    18361 cgccgcagcc ataataccta tcggacctgc accagaaacc agcacatctt cgcccaccag
    18421 atcgaacgac agcgcggtat gcacggcgtt accgaaggga tcaaaaatgg aagccaggtc
    18481 gtcggagata ttgtcgggga ttttgaaagc gttgaacgcc ggaatcacca gatattcggc
    18541 gaagcagccc ggacggttaa caccgacacc aatggtgttg cggcacagat gggtacgacc
    18601 gccacgacag ttgcgacagt gaccgcaggt gatatggcct tcgccagaaa cgcgatcgcc
    18661 gattttgaag cctttcactt cctgaccaat acctaccact tcaccgacat attcatggcc
    18721 cacgaccatc ggcaccggga tggttttttg cgaccactca tcccagttat agatgtgaac
    18781 gtcagtcccg cagatggctg ttttacggat tttaatcagc agatcgttat gcccgagttc
    18841 cggtacaggg acgtcggtca tccagatgcc ctcttccgct ttcagtttgg ataacgcttt
    18901 catctcacat cctcaggcga taacgcccag ttgtttacca atacgcgtga atgcttctac
    18961 tgcacgcgta atttgctcag gggtatgcgc cgcagacatc tgggtacgaa tacgcgcctg
    19021 acctttcgga acgaccggat agaagaaacc ggtgacgtaa atgccctctt tttgcagctc
    19081 tcgggcaaat ttctgcgcca ctaccgcatc accaagcatg accggaataa tggcgtgatc
    19141 ggctcccgcc agggtaaagc ccgccgccga catttgctca cggaactgac gcgcgttcgc
    19201 ccacagacgg tcacgcaact cgctacccgc ttcaaccatc tccagcactt tgatggaagc
    19261 ggcgacaatc gctggtgcca gcgagttgga gaacaggtac ggacgagaac gctggcgcag
    19321 ccactcaacc acttctttgc gcgccgcggt ataaccacca gaagccccgc ccagcgcttt
    19381 accgagcgtg ccggtgatga tgtcgacacg ccccatcaca tcgcagtatt catgggaacc
    19441 acgaccattt tcaccgacaa aaccgaccgc gtgggagtcg tctaccatca ccagggcatc
    19501 gtatttatct gccagatcgc aaacgccctt cagattggca atcacgccgt ccatcgagaa
    19561 cacgccatcg gtggcaatca gcacatgacg cgcaccggct tcacgcgctt ctttcagacg
    19621 atcttcaagc tcctgcatat cgttgttggc atagcgatag cgtttagctt tacacagacg
    19681 cacaccatca ataatagacg cgtggttcag tgcgtcggag ataatggcgt cttccgcgcc
    19741 cagcagcgtt tcaaacaggc caccgttagc atcaaagcag gaagagtaga gaatcgcatc
    19801 ttccatcccc aggaatgccg ccagtttttg ttcaagctct ttatggctgt cctgagtgcc
    19861 gcaaataaaa cgcaccgaag ccatgccgaa accgtgagaa tccattcccg cctttgctgc
    19921 cgcaatcaga tccggatgat tcgccagccc gagatagttg ttggcacaaa agttaatgac
    19981 gtggcttcca tcagccacgg tgatatctgc ttgctgcgca gacgtaataa tgcgctcttc
    20041 tttaaacaac ccttccgccc gtgcggtttc cagatcgttg gttaactgct gataaaattc
    20101 tccacgcatt gcgattctcc agacagggca aattccagca catattaccc aaacttatag
    20161 gtcgcgacga gatgatgcgt taacacttct gcaaaattca ggataaataa cgtgtatctt
    20221 gatgtctctc aacacctaca gatagtagaa aaactgtaca gttttggctg agcataatga
    20281 tgagtgattt tactgcattg atataaaaag aaaaaatgat gccaaagcat catttttcct
    20341 atctcagttg gcatcaaaac gctacccact aatggaggga aacctatttt cttgaaagga
    20401 aaattctcag cgcctgcatt tttgccccta aagtgttact cctgaagcag cgaaacaaat
    20461 cgaaccattt tcctctcccc agatagttta ggttgaagag atcggaacgt ttgtaatagc
    20521 acatgacaaa aatgccctgg tcatcgtcca caatgttatt gtttagtgtt attttttgac
    20581 tttccagaac taatttatag aattctttcc atttatgctg tgaaccaaca attgcgcccc
    20641 caataatata tacatgattg ccaatcatga aatcaaaagc ctgttgttga gatgtaacag
    20701 taagcccttt ttttatagtg aaaagatgca tcttattttc atcaaatggg aaatcccaaa
    20761 ttttcaatcc tcgagttaca tttggcttac ggcaatagcc aaaatctatc cacgcaacaa
    20821 gaggcgtttt cactaaaccc atgttgatag ctttatttac aaaataggcc ttaaggttac
    20881 ataccaaaac atactctggt gaccagtact ctgggttttt taattgtcga ggctcaagtc
    20941 tatttgtaaa cgactcatct ttttgaattt tttcgatacg atttctgata tatctaaatt
    21001 tcttttttat atcgataacg ataaccgttg ttggttttcc gttgcgaatc gcttcaatcc
    21061 ttggtttcaa atcgggagaa gtgaaaataa tcatctcatt ttcaagtgcg gccagacgct
    21121 caaagtaact aaaataaaca tcaactgaac gagctagttt ttcacggaac cctttattag
    21181 cagtccaatc ccctctacca atgtcaaaat atgcagtgat aattgtggtg gatgatttca
    21241 ttactaataa tgaccattaa tataaaaaaa gaattatatc ataaatatta acagaatgta
    21301 tactgtatat tatccattca aatgattccg ttatttatca tacatcggaa tattgatact
    21361 aaagcactat tcacatgcaa aaccaacatc cgccatgaag gactagctaa aacccaaact
    21421 agtttgttgc aattagcatc cttgcacctc tatgtaaagg gctgaaggga ttcggatgtg
    21481 atggtatgat tacagacatt cgtgtctgag attgtctctg actccataat tcgaaggtta
    21541 cagttatgat catcgttacc ggcggcgcgg gctttatcgg cagcaacatc gttaaggccc
    21601 tgaatgataa aggcatcacc gatattctgg tggtggacaa cctgaaagac ggcaccaagt
    21661 ttgtgaacct ggtggatctg gatatcgcgg actatatgga taaggaagac ttcctgatcc
    21721 agattatggc tggcgaagag ttcggtgatg tcgaagcgat tttccacgaa ggcgcatgct
    21781 cttccaccac cgagtgggat ggcaagtata tgatggataa caactatcaa tactccaaag
    21841 agctgctgca ctactgtctg gagcgcgaaa tcccgttcct gtatgcctct tccgcagcca
    21901 cctacggcgg acgcacctcc gactttattg aatcccgcga gtacgaaaaa ccgttgaatg
    21961 tctacggtta ctcaaaattc ctgtttgatg aatatgttcg tcaaatcctg ccagaagcga
    22021 actcgcagat tgttggcttc cgctatttca acgtttatgg accgcgtgaa ggccataaag
    22081 gcagcatggc gagcgtcgct ttccatctca acacccagct taataacggt gaatcaccga
    22141 agctgttcga aggtagcgag aacttcaaac gtgacttcgt ctatgtgggc gacgtggcag
    22201 atgtaaacct gtggttcctg gaaaatggcg tttccggtat cttcaacctc ggcaccggtc
    22261 gtgcagaatc cttccaggca gtagcagatg ctacgctggc ttatcacaag aaaggccaga
    22321 tcgaatacat tccgttcccg gataagctga aaggccgcta ccaggcgttc actcaggcag
    22381 atctgacaaa tctgcgcgcg gcgggttacg acaaaccgtt caaaaccgtt gctgaaggtg
    22441 taacggaata catggcctgg ctgaatcgcg acgcataaga gctctgcatg aaaatactgg
    22501 tgatcggccc gtcttgggtt ggcgacatga tgatgtcgca aagtctctat cgcacgctcc
    22561 aggcgcgcta tccccaggcg ataatcgatg tgatggcacc ggcatggtgc cgtccattat
    22621 tatcgcggat gccggaagtt aacgaagcta tccctatgcc tctcggtcac ggagcgctgg
    22681 aaatcggcga acgccgcaaa ctgggtcata gcctgcgtga aaagcgctac gaccgcgcct
    22741 acgtcttacc aaactccttc aaatctgcat tagtgccttt cttcgcgggt attcctcatc
    22801 gcaccggctg gcgcggcgag atgcgctacg gtttactcaa cgatgtacgc gtgctcgata
    22861 aagaagcctg gccgctaatg gtggaacgct atgtcgcgct ggcctatgac aaaggcatta
    22921 tgcgtaccgc acaagatctg ccgcagccat tgttatggcc gcagttgcag gtgagcgaag
    22981 gtgaaaaatc atatacctgt aatcaatttt cgctttcatc agaacgtccg atgattggct
    23041 tttgcccggg tgcggagttt ggtccggcaa aacgctggcc acactaccac tatgcggaac
    23101 tggcaaagca gctgattgat gaaggttatc aggtggttct gtttggctct gcgaaagatc
    23161 atgaagcggg caatgagatt cttgccgctt tgaataccga gcagcaggca tggtgtcgga
    23221 acctggcggg ggaaacacag cttgatcaag cggttatcct gattgcagcc tgtaaagcca
    23281 ttgtcactaa cgattctggc ctgatgcacg ttgcggcggc gctcaatcgt ccgctggttg
    23341 ccctgtatgg tccaagtagc ccggacttca caccgccgct atcccataaa gcgcgcgtga
    23401 tccgtctgat taccggctat cacaaagtgc gtaaaggtga cgctgcggag ggttatcacc
    23461 agagcttgat cgacattact ccccagcgcg tactggaaga actcaacgcg ctattgttac
    23521 aagaggaagc ctgacggatg cgggttttga tcgttaaaac atcgtcgatg ggcgatgttc
    23581 tccatacgtt gcccgcactc actgatgccc agcaggcaat cccagggatt aagtttgact
    23641 gggtggtgga agaagggttc gcacagattc cttcctggca cgctgccgtt gagcgagtta
    23701 ttcctgtggc aatacgtcgc tggcgtaaag cctggttctc ggcccccata aaagctgaac
    23761 gcaaagcgtt tcgtgaagcg ctacaagcag agaactatga cgcagttatc gacgctcagg
    23821 ggctggtaaa aagcgcggca ctggtgacac gtctggcgca tggcgtaaag catggattgg
    23881 actggcaaac cgctcgcgaa cctttagcca gcctgtttta caattgtaag catcatattg
    23941 caaaacagca gcacgccgta gaacgcaccc gcgaactgtt tgccaaaagt ttggactata
    24001 gcaaaccgca aacccagggc gattatgcta tcgcacagca ttttctgacg aacctgccta
    24061 cagatgctgg cgaatatgcc gtatttcttc atgcgacgac ccgtgatgat aaacactggc
    24121 cggaagaaca ctggcgagaa ttgattggtt tactggctga ttcaggaata cggattaaac
    24181 ttccgtgggg cgcgccgcat gaggaagaac gggcgaaacg actggcggaa ggatttgctt
    24241 atgttgaagt attgccgaag atgagtctgg aaggcgttgc ccgcgtgctg gccggggcta
    24301 aatttgtagt gtcggtggat acggggttaa gccatttaac ggcggcactg gatagaccca
    24361 atatcacggt ttatggacca accgatccgg gattaattgg tgggtatggg aagaatcaag
    24421 tagagtgtcg ttctacaagt atgtctcttg cagatttgcc agctcaaacc gtttttcaaa
    24481 atttaaatct tgaaataata accaataagt tgacatcgga gataagatga cctcaacatt
    24541 atttttctct ctcgagaaaa aaaactggat agcgtactgg aacagagctc tcgtattctt
    24601 attcattacc acctattttt tgggtgggat tacaaggtat aaacatctta ttgttattct
    24661 tatgacaata acgacaatcg tctatctctg caaacggcca aaacactatc tctcactatt
    24721 taaaacattt ctttttggta gtgttgccat attaactatt gctgcattgc tgtcacttct
    24781 tcaatcccct gatgcaggtg ctagcatgaa ggaaattttc aaagctatta ttgagaatac
    24841 tttactatgc acaatagcaa taccggtcat attgagagac gagaaaagag aagatgtcga
    24901 aaaaatcgtt ttcttctcat ttattagtgc gttgggctta cgctgttttt ctgaattgat
    24961 tacctattat aaggactatc aacaagggat aatgccattc gcagattata gacaccgtag
    25021 catttctgac tcgatggtct ttttattccc tgcattgtta aatctctggc ttatcaaatc
    25081 agcaaaatac cgcatttctt ttgtggttct aagcgttatt tttatttttc tgatattagg
    25141 aactttatcc agaggggcct ggctttccgt gttagtcatt ggattaatat ggattctgat
    25201 gtttaaacaa tggaagttac tattagtagg agtaatggtt tccatcattg cattgtcggt
    25261 tattttcaca cataaggaga tgaccgcaaa gctaacgtat aaacttcaac aaactaatag
    25321 ttcttatcgc tatgcaaatg gtactcaagg cagcgcactc gatctaatat tagaaaatcc
    25381 tgttattggt tatggttacg gtaacgttgc atataaagat gtctataata aacgtgtcat
    25441 tgattatcca gaatggacct ttagacaatc aatagggcca cataattttg cgctattcat
    25501 ctggtttggc actggtttat tagggctggt aagtcttatg atgctatact gtgcaatatt
    25561 gaaagagtgt ataaaaaatg gcgtcaagaa taaatatcgc tcaccatata atgcatatta
    25621 tataatctta ctatctttta taggttattt tgttatccgt ggaaacgtag aacaaattga
    25681 accaaattta ttaggcgttt acgccggctt attattagcg atgaaaaaca agtaaaaaat
    25741 aaaaaaggct gcataatgcg gcctttttat ttattcaaac caattatgaa taacctcttc
    25801 gaatctttga gttacgccat cccaactgta gtgatcaaac acaaagtcct gaccttgctt
    25861 tgcaactgct gttaattcag gattcgctaa cgtttttaaa atatcgctgc ttatggagtc
    25921 tgcagtcata ggttctttta aatggaatcc cgtagtattt tctttgacaa attccgtcat
    25981 ccccccacgc gtactcacta aaacaggctt accagcaccc atagcttcaa ttgccaccat
    26041 gcaaaatggt tcctggaact gggaaggaat gacgactaaa tctgccagtg ggtaataaca
    26101 atgcatcttt tcaggcggta tgcttcctaa cataatgcaa cgatccttca aacgttttgc
    26161 aatttccctg acattgcgtt gatatgcacc tttgtcactt ttactcattt ctgtataatc
    26221 accaatcaca actaatttta aatttttgtg agcagcggcc aatttttcga atgattgcat
    26281 caacagcaat atgcctttgt cagggacgat acgcccagca taaaaaataa ttttctcttc
    26341 aggggagata ttaagttcgc ttctagtgat cggctgaaaa ttagattgat aggtttctaa
    26401 atcaattcca tttggtacaa tctcaatatc tgcatttgct aaataagatt ggtaatactt
    26461 tttcaagtat aaactaggga ctatcatttt aacattttgc tcaagcaatt ttggttcaaa
    26521 tgcattatgc atatgtataa ccactcttgc ctgaggagct cgcttacgaa tttgagtgta
    26581 tagcttcatg ctattatgaa ctacaataac actttctttc gttataggaa aatcatgtgc
    26641 aatattcaat attctctgtg aatatggaag aggatcaagt cttgtccatt tttgaaataa
    26701 ccgcttatat atgcgactga atccaaccct atgaatacta caattatcat taacaaaggt
    26761 gtaatttgag tatcctggat ttttgataca aacaatacga tttggaaaat tggtacgctg
    26821 agcaacctga tacatccacg tttcaacagc agccgctccg cgaggaggaa ttgaaaaaat
    26881 aggagtaacc gtaaatatta ttttatcaac catctcgtat attaaccttt cataacatta
    26941 tatttaattg ctgtaaaaac accatcgaga aattttttct ggtaaagcaa gtgtttatat
    27001 ttttctttgt gctcatgttt tttaacagct tttttataag gtatatttct ccatggtgat
    27061 atattataaa tattacgaaa ataatctgca gaggcgtagt tcgcccaatc atgccatggc
    27121 ttagttatac cagtataatg tatgaagaca gtgtcatcat taataaaccg ggtgtaatat
    27181 tcactatttt tttcttcaaa ttctgacttt atagtataaa tacaattata ttttctcggt
    27241 aatattttag catgatgtag aaacattaag tttaaaatat cttgatccgg gtactttaat
    27301 ttttttataa ttgactcatc tgataataga tcaaagaatt tttccgttag tctttgtttt
    27361 aaccattccc gtaaattaat atacattaca ccagagttaa aatagctacc gttaaaatcc
    27421 tcattacaca aacgctcagc gcttttactt tgcatagcat ctacatcaat taccactgcc
    27481 ccatattcat ctttaaactc taatgctatt aactcgttca atgaaccctt acagacgata
    27541 tcagcatcaa gatataataa tgaatccaat cgcgcagaaa aataatcaaa tgaaagcaaa
    27601 cgataataaa tagatactgg ccagatattt gattgaggta gcgccttcaa tggctcagaa
    27661 tcaatatgat ataatttaat tactgtttga tacttcgttg ctaattgtga taataattca
    27721 atatactctt tgtctaagta atcagcgatt atgtgaaagg taaatttgtt ttgtttattc
    27781 ttttcaagaa ttgaagcaat tgaaatcgca gccccaacct ggtagttctc atcaataccc
    27841 catgagacat taaacgtttc ccgttcatca agtttaacgg ggcgcttgtc cagttcaatt
    27901 atatctttaa attgagtaag atgtttaaaa tccaatttaa ttacccttca tcataatcat
    27961 tatttgctct tcaatcctag tttgactttt acatccctga ttactttttt aattttcttt
    28021 ttgaatataa gatatgtata gccaaagtct ttcaattcat tttttatatt atagtgccgt
    28081 tcaagatcaa tgcgatcttt tgcttttaaa acagcggttg tttttttacc tgaaaggtca
    28141 attagtctta aacctttttc ggatactata aaatttcctt tatgaggatc gccagacacc
    28201 attccatgct gatgcaactc ttttatcgat tctgataatt gatccttcag atcttcagaa
    28261 atttccaaat actcatttaa cccgacacct tcaatatact caatgagcat aatataataa
    28321 tgagcgaaat ttaatgtttt acgttcagct agcaaaaaat agtcattaat tgattggatg
    28381 ccttcaccac gtacccgatc ggtttgataa attaaatttt cataataatc tttttttatg
    28441 caagatttta agaaacgttc ggtcatttta tgctttggcg cataaacttt aagtacaaga
    28501 ggtccacgag cagtgtcaat aagaatgact ttagtatcat caatagaacg aaaaactttt
    28561 aatgttttaa ccctgcaagt taaaaagtca tttagaactt gctcataaaa gggatcatta
    28621 tcttttatga aaactttcaa cccatttatc gttttattat atatcataat gaattatttt
    28681 aaccttaaat ctttagaagc atttttcttt ataatacttt aaataattaa aaatgcctgc
    28741 catataatgc ttttgtttaa atttatgttt tgcacaatac cgatactgat tgctatttac
    28801 aggtttaagt aaatcttctt tactccacgg agaagctgct ttggcgatca aaaaactacg
    28861 tgaaaccgga taatcagccc actcatgcca gggttttgta ggtcccacat agtgtataaa
    28921 cacggtgtca tcattgactg gattatcaac tttgtctttt aattcatagt taatactata
    28981 tcgggtatta tatttctccg aaataaactt cactttacca ttcaataata cattcagtac
    29041 atcttgatca aggtgggtga ttttacttac ccaatctggg tcacgcaaca tttcaatagc
    29101 ttttgacgaa atgttattta gattccactc atcaatattt atcagtaaaa aaccagcatt
    29161 gaaatatcca gaagctaact gtggtgtagt taataccgag gctcgatttt gccaccattc
    29221 aatatctctt tcagctacaa ctgcggcaat ttcattagta gaaaattgat aatctaagag
    29281 ttctttaata ctacccttgc aagcaatatc tgcatcaaga tatagtattt tttcatgttt
    29341 atgatagaaa taatctgcaa ttataaatcg aaaatatgtt gcgtaagtcc agtttttcgt
    29401 gcttggtaat gacttcagct tatcacaatt gataacataa atattaattc ttgaattata
    29461 ttgttttgct aaatcagaaa aatataattt gtctttatca ctgatataat ctgtgaaaac
    29521 atgaaattca caagagattt ctctattgtt taatagaata gatgcgatgg ctacaccaca
    29581 tccaaaaaga aaatttctgt caataccaaa tgctatatca agagtatttt tagatcttat
    29641 attttgataa tgaaaattat actcaaaaag gatgatgtca ctatcattga gttgagacat
    29701 atttattctg tatccaaaat tttggcaaaa ataagttacg ggttacaacg attttcgaat
    29761 cgttctttcc ctgatttttg tggcttttgc ttctgcttgc gacagcagtc cttgttcctg
    29821 cttgagaatg tctttaagcg gggcggcaaa atacaccttc ataaagcgcc agatatcccg
    29881 ctgagtcagg ccaatattca ttgaagaaaa ataaagccct ataagatctt tatcgcgcca
    29941 acgacctgga acgcgcgtgc gaagctgcgc ccggtgtagg tcaattaccg aaatttttaa
    30001 ctcctcttcc ttaccggaaa aaggcaagtg cagcaggaaa tgacagatat aacagtcacg
    30061 atggttaatg cccgcagcat gcatatcgcg caccatcgtc gcgacacgct taataagcat
    30121 acgctttacg cgaacatctg gtgggttggt cgcccagtca gcacagtaat cttccagact
    30181 gatggttggt gtcagatctt cggtaataat aaacgaggtt ctggtcagcg gattcatgcc
    30241 tttttcgccg aatgccaccc catacatagt atcaacgccg acatcccgca gtcgatgaat
    30301 cgcattccat tcgcggtctg cgcctaatac tggcatccgc aatgagagta aatttttgat
    30361 tatctctttc agggtcgtgc cgcgatgcca tttgagaaaa tagctttttc ccgccatttc
    30421 aaagcgcaga gtacggcgag tttccagttc acgaaatacc tcaccctgca aggttttaac
    30481 ttcctcaaaa ggatctttgc cgcgccataa cgtggcaaac ggctctttaa gttcaaccat
    30541 ccagaccacc cgttatgata tccgccgctt tctctggcag actgtataaa tcttgtgtat
    30601 cagcataatg tcgcgcattt tccgcccaag cctggcgcaa tgaagattgc gttaacgctt
    30661 tgcgtaaaat ctcattcaat gtttcctggc ggaatggctc agcaatagcc tcgccgcaat
    30721 tagcgtcgac aatataatgc gcatagccac aaacggcagt tgttagtacc ggtaatcctg
    30781 cagtaatcgc ttccagcagc acaattcccg ccgcttcctg gtaggcagga tgcagtaata
    30841 aatccgccgc cgccattaat tccgagacat cgttgcgccc cgagaagaag tgaacattac
    30901 tgcgcacgcc gcgtttttct gccagtgcct caaattttcg cggtttatcc tgcccaacaa
    30961 catatagcaa tgtgttgtgg cgcagcgaat ccggtaacga agcaagtgct tcaatggaac
    31021 gatcgacacc tttacgcgtg aagtctgaac cgacctgcaa caataaatat tgttgttcgg
    31081 ttattccatt cttcttacgg aagatttcac ggctattggc tggctgctgg ctatatttac
    31141 gatcaggata aatccccggt ggcagaatat gaaaacgctc cgcttcagtc tgataatgtt
    31201 tctggaaatc ggcgatttgc ttatctgtca gcatcagcag ctgtgtcggc ttgccctgtt
    31261 cgaaggttgc ccgctcaaag gcggcataat ggcgataacg tgacgtcagg cgatagaaaa
    31321 agcctttttc ctgcgctact ttctcggcat aacaaacatc agcggcataa taaacgtcca
    31381 gccccggcat tttgttgaat ccaacgactt tatcgacggg atgttcgcgt aaatgttttt
    31441 gcacccaggc aaaatactcc gcattgcgcc cgtgattggt atgcgattta accggcactt
    31501 tgatcagttc aaatacatca gggcattcgc cttcccacga ctgggtataa acccgaacat
    31561 gatgacctcg ggcggcgact gtctgagcaa tacgcataaa atcgcgctgc aaaccgccaa
    31621 agggaaaata tttatataaa caaaaagcaa cgatcataac gacgtacctg tcgtggagga
    31681 gggcagtaat ttatcgacag cagcaatgac atctgccgcc ggaataaccg aaagatacat
    31741 ctcatttcgg tcacgctgat cgcgcgttgg catttcccgg taatctcccg cccagaattg
    31801 aatcatgtta tttgaccagg gacgccagaa aatatgatct gttgcgccaa acagcgatat
    31861 cagcggcgta ttaactgcag cggcaatatg cgccggtgcg gaatcaacgc caataaacag
    31921 ctgcgcatga tcgattaacg caccaagttc cgggaaggtc acctttccag ccagcgccgt
    31981 tactggtggc gtctggcatc cctgcgcaat ttcattgacg caggccagat cgtctttatc
    32041 tgggccggac gtcagaacga cttcataacc acgagcatgt aaggcatcaa tcacagcgga
    32101 aaacttggcg ttgtcccagc atttgaagat ttgccgcgcc gtaggttgga taaccacata
    32161 attttgtcca acaccagcgt gatcaagttc gcgacgcata cgtttccagc ttgcaggcgg
    32221 gtaactcatg gttgtctgct tcaccaacga atcaagtccc aatggtgtca gcacggataa
    32281 gttactttcc accacatttc caccctgcaa cggcaccaaa tgggtgaaac ttttacgcca
    32341 aaaagcagac tgccgatgat gataatcctg ggaaattttc acacgagcat ttaataagcg
    32401 aaccagtata gcaaccatcc attgatcggt aagattgacg ataaggtcat acttattggc
    32461 acgtaatacc ttgatgagat gaaaaaagtt ggcaattttt tctgaggctt ttgctttttt
    32521 attttttatg ccgtagagcg cgttaatctc tggattttca gacaggatcg ggatggtgtc
    32581 ctgataaagc agcacatcga tttttgcgtc agggtaattt tttttcagag aactaatgac
    32641 gggagtagtt aataacatat ccccatgaaa acgcatctta atgagcaaaa ttcttcgaaa
    32701 tggcttatcc acaagtaact ctttttgttg agattatccg attaaggtga agcagaaaaa
    32761 gcacgctacc gccccaggct caacagctac ctgaatactg ataccgcact gatattgacg
    32821 cgctagtgta tcacttcttt cgcggcaatc cgacccgaat gatttttaag cacattgttg
    32881 attttcaata aagaaatcaa tcaaataaaa tcattagcaa ccatattctt tcactgttgc
    32941 tctgcgattc cttcctgcac gtacagcaga aatcaatgaa tcgcataaaa atacaaaaaa
    33001 agtatagatg ttcagcaaga attggcgggt aaattgctat ttaa
//
LOCUS       DADMEQ010000034        32723 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.034, whole genome shotgun sequence.
ACCESSION   DADMEQ010000034 DADMEQ010000000
VERSION     DADMEQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.034"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            523..1155
                     /locus_tag="JNZ05_001638"
     CDS             523..1155
                     /locus_tag="JNZ05_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HAY8564911.1"
                     /translation="MKNLPIGGVWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFP
                     EFAHATPRVQLDLHPTGSVNGSNYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAA
                     GRRDGYFSIYRQGGTTTDEGERIDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRP
                     VVLPGIRYAVMCVPTPLTLAVDKFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(1243..2541)
                     /locus_tag="JNZ05_001639"
     CDS             complement(1243..2541)
                     /locus_tag="JNZ05_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8564912.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            3024..4436
                     /gene="uxaC"
                     /locus_tag="JNZ05_001640"
     CDS             3024..4436
                     /gene="uxaC"
                     /locus_tag="JNZ05_001640"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAY8564913.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSSRGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            4451..5938
                     /locus_tag="JNZ05_001641"
     CDS             4451..5938
                     /locus_tag="JNZ05_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAY8564914.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            6021..6572
                     /locus_tag="JNZ05_001642"
     CDS             6021..6572
                     /locus_tag="JNZ05_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HAY8564915.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(6577..7821)
                     /gene="sstT"
                     /locus_tag="JNZ05_001643"
     CDS             complement(6577..7821)
                     /gene="sstT"
                     /locus_tag="JNZ05_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAY8564916.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(8417..9382)
                     /gene="alx"
                     /locus_tag="JNZ05_001644"
     CDS             complement(8417..9382)
                     /gene="alx"
                     /locus_tag="JNZ05_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HAY8564917.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(9653..10669)
                     /locus_tag="JNZ05_001645"
     CDS             complement(9653..10669)
                     /locus_tag="JNZ05_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY8564918.1"
                     /translation="MEYPRVMIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQ
                     AQHFANDFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPL
                     ASNLAEVDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSS
                     RYQRYLDGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAH
                     GVVVMDYGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDL
                     TQPQHINTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL
                     YLPK"
     gene            complement(10730..11422)
                     /locus_tag="JNZ05_001646"
     CDS             complement(10730..11422)
                     /locus_tag="JNZ05_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HAY8564919.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(11499..12002)
                     /locus_tag="JNZ05_001647"
     CDS             complement(11499..12002)
                     /locus_tag="JNZ05_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY8564920.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRMQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            12087..13223
                     /gene="rlmG"
                     /locus_tag="JNZ05_001648"
     CDS             12087..13223
                     /gene="rlmG"
                     /locus_tag="JNZ05_001648"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HAY8564921.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            13508..13822
                     /locus_tag="JNZ05_001649"
     CDS             13508..13822
                     /locus_tag="JNZ05_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase HigB"
                     /protein_id="HAY8564922.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            13819..14235
                     /gene="higA"
                     /locus_tag="JNZ05_001650"
     CDS             13819..14235
                     /gene="higA"
                     /locus_tag="JNZ05_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HAY8564923.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(14280..16298)
                     /locus_tag="JNZ05_001651"
     CDS             complement(14280..16298)
                     /locus_tag="JNZ05_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HAY8564924.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLIGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVHERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLQLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(16524..18875)
                     /gene="ygjK"
                     /locus_tag="JNZ05_001652"
     CDS             complement(16524..18875)
                     /gene="ygjK"
                     /locus_tag="JNZ05_001652"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAY8564925.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(18892..19963)
                     /gene="ygjJ"
                     /locus_tag="JNZ05_001653"
                     /pseudo
     CDS             complement(18892..19963)
                     /gene="ygjJ"
                     /locus_tag="JNZ05_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(20097..21530)
                     /locus_tag="JNZ05_001654"
     CDS             complement(20097..21530)
                     /locus_tag="JNZ05_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8564926.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGDVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(21593..22042)
                     /locus_tag="JNZ05_001655"
     CDS             complement(21593..22042)
                     /locus_tag="JNZ05_001655"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HAY8564927.1"
                     /translation="MRIIDNLEQFRQIYASSKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(22039..25125)
                     /gene="ebgA"
                     /locus_tag="JNZ05_001656"
     CDS             complement(22039..25125)
                     /gene="ebgA"
                     /locus_tag="JNZ05_001656"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HAY8564928.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAYYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIIPTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHDNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(25307..26290)
                     /gene="ebgR"
                     /locus_tag="JNZ05_001657"
     CDS             complement(25307..26290)
                     /gene="ebgR"
                     /locus_tag="JNZ05_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HAY8564929.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELNN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            26509..26840
                     /locus_tag="JNZ05_001658"
                     /pseudo
     CDS             26509..26840
                     /locus_tag="JNZ05_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(26882..28324)
                     /gene="ygjG"
                     /locus_tag="JNZ05_001659"
     CDS             complement(26882..28324)
                     /gene="ygjG"
                     /locus_tag="JNZ05_001659"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HAY8564930.1"
                     /translation="MITAFVFIPIFAIAAGVAQSLQYLNRYHVIREPPEHILNRLPSS
                     ASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGA
                     VEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLD
                     PLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFH
                     GKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEP
                     IQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILC
                     LAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLDGFR
                     QLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEP
                     PLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            28630..30150
                     /locus_tag="JNZ05_001660"
     CDS             28630..30150
                     /locus_tag="JNZ05_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY8564931.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMFTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNSGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRTRGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(30304..30927)
                     /locus_tag="JNZ05_001661"
     CDS             complement(30304..30927)
                     /locus_tag="JNZ05_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAY8564932.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            31204..31950
                     /gene="nfeF"
                     /locus_tag="JNZ05_001662"
                     /pseudo
     CDS             31204..31950
                     /gene="nfeF"
                     /locus_tag="JNZ05_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(32004..32079)
                     /locus_tag="JNZ05_001663"
     tRNA            complement(32004..32079)
                     /locus_tag="JNZ05_001663"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32044..32046),aa:Ile,seq:cat)
     gene            32204..32710
                     /gene="mug"
                     /locus_tag="JNZ05_001664"
     CDS             32204..32710
                     /gene="mug"
                     /locus_tag="JNZ05_001664"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HAY8564933.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT         7945 a         8499 c         8610 g         7669 t
ORIGIN      
        1 tcaggcacag ttgaaacagc ctaaattacg tggaaacgtt cagacggcgc aaagggaggt
       61 tgcaccatga gaaatcgatt gattgcggcg atattgggct tgtttggcac gctcactggc
      121 gttcaggcag ctcctgacgt gaccagtgaa attacgtatg atttggcatc tggtagagcg
      181 gattattact tctggaagga tgaagcgtcg gcaggaaata atggatatat gtggtatgaa
      241 tgttcgtatc ctgacctcca acaaacctgt acagctaatg gaaatatatc gacagtacaa
      301 atctatttaa ctgaacaacg cagtgggatg cgttggccgg taaaactcaa aggatttaaa
      361 acagccattg taagtagcga tgaagcgccg ctaggatgca aggggggcaa agggcttcag
      421 acgaatctta aggattctaa tagatcttca tgtacagaag atggtcaaca ttattatata
      481 tacgatacaa agtttcttac gctctacctt gagcagacag agatgaagaa tttgccgatt
      541 ggtggcgtct ggaaggggaa agttaaatta cattcgaaca gcccggccca ggactatttc
      601 gcaaatatta ccctgaatac gctcgacccc aaccatattg acgtgttctt cccggagttc
      661 gcccacgcca cgccaagggt gcagttagac ttgcatccaa caggaagcgt taacggcagc
      721 aactacgcgc aagatctgac catgttggac atgtgcctgt acgatggttt taacggtaat
      781 gccatcagtt atgaaatcat gctcaaagat gaagggcgac cagctgcagg gcgcagagac
      841 ggttacttct ctatctatcg tcagggaggg accaccaccg acgagggaga acgcattgat
      901 taccgggtca aaatgtacaa cccggaaacc ggtgggcaaa ttgatgtgcg caataatgaa
      961 aatatggtct ggaacagcat taacctgaaa cgtgtgcgtc cggtggtact gcccggtatt
     1021 cgctatgccg tgatgtgtgt gccgacaccc ttgactctgg cagtcgataa attcagcgtg
     1081 atggataaac aggccgggta ctacatgggc aaattgtcag taatctttac gccttccttg
     1141 ccaaccatca attaaccctg caaaccggac aaacgctttt ttctatataa acgacgaagc
     1201 cgcctgataa ggcggcttcg tcgttataac ttacgttaaa cgttaatgtt gcggtgcggg
     1261 atcgttatgt gtttcctgcg ccacctcaat cgccggtttg ttctgcaata cggtccagat
     1321 aaccagcgca cccagcaggt caaatactgc cagaactgcg aacagcgggc tgaagccgat
     1381 ggtgtcagcc agtgcaccga caaccagcgc aaacagcgta cttgccagcc atgcggacat
     1441 cccggttaaa ccgttcgccg tcgccacttc gttacgaccg aacacgtcag aagagagcgt
     1501 aatcagcgcg ccagacagtg cctggtgggc aaaaccaccg atacacagca gcataattgc
     1561 gacatacggg ttggtgaaca gaccgatcat acccgggcca atcatcagca cagcacccag
     1621 cgttacgacc atcttacggg aaacgatcag gttcacacca aaccaacgct ggaacagcgg
     1681 cggcaggtaa ccaccgagga tacaaccgag gtcagcaaac agcatcggca tccaggcgaa
     1741 catcgcgatc tctttcaggt taaagccgta aactttaaac atgaacagcg ggatccacgc
     1801 gttaaaagta ccccaggccg gttctgctag gaaacgcggc agtgcgatac cccagaactg
     1861 acggttacgc aggatctgac caacggacat tttcttcgcc gtgctcacct ggtgctgggc
     1921 ttcctgacca ttaataatat agtcgcgttc ttcatcggtc agatgcttct ggtcgcgcgg
     1981 atgtttatag aaaatcagcc atgccatcgc ccagataaag ctcaatgcac cggagatgat
     2041 aaatgccatc tgccagctgt gcatcacgat tgcccatacc accagcggcg gcgcaatcat
     2101 cgcaccaatc gaagaaccta cgttaaagta gcctactgcg atggaacgct ctttcgccgg
     2161 gaaccattcg gagctggctt tcagacccgc cgggatcatc gctgcttccg cggcaccgac
     2221 cgcaccacga gcaacagcca ggccacccca gctacctgcc agcgcggttg caccacagaa
     2281 cacggcccac agcacagcaa acattgcata accgattttc gtacccagca catccagtac
     2341 ataacctgct accggttgca tgaccgtata agcagcagaa taggctgcga tgatatagga
     2401 atactgttgg gtggagatgt ttaactcttc catcagagtt ggcgcagctg ccgccacagt
     2461 gttacgcgtc aggtaaccaa gcacggtgcc gagcgtcacc agtgcgatca tataccaacg
     2521 taacccttta attttacgca tgtaaaacct catcgttttg ttatatccgc aggaagcgcg
     2581 gccatcatcc cgccagaggc tgaagatgtt agttacgacc gggtaaccga aaaaaaaccg
     2641 gcacgccccg aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc
     2701 cgattcatct aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga
     2761 cggcagaaag ataacttgtc atacaacctt aaaaggtgag agccatcaca aatgtgcgaa
     2821 tatttgtagg gacattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga
     2881 tttaccactt tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc
     2941 ttcggactct ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct
     3001 cactcgttga gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata
     3061 ccgaatttgc ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc
     3121 attgccattt gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca
     3181 tctggttgaa gggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggctgagc
     3241 gtctgtgtac tggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc
     3301 cgcacactat cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgttcg
     3361 gtatcactgg caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg
     3421 aactgctggc gcaggataac ttctcctcgc gcggcatcat gcagcagatg aacgtgaaaa
     3481 tggtcggcac caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag
     3541 acggctcttt caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaatatcg
     3601 aacaggcgac ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc
     3661 gccgctttgc tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg
     3721 gctgtaaagt gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg
     3781 aactcgacag cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg
     3841 cacagttcaa aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg
     3901 tacagcagta ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg
     3961 ggccggatgt aggctttgac tccatcaacg accgtccgat ggcggaagag ttgtctaagc
     4021 tgctgagcaa gcagaacgaa gaaaacctgc tgccgaaaac cattctctac tgcctgaacc
     4081 cgcgcgataa cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg
     4141 gcaaaatgca gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc
     4201 agatgaccca actggcgcag ctcggtctgc tgagccgttt tgtcggtatg ctgactgaca
     4261 gccgtagctt cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga
     4321 tcggtcgctg ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg
     4381 tgaaaaatat ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc
     4441 tggggttgat atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc
     4501 agatttggct gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga
     4561 tgttgctcgt ggacataaat ttgcgttaac ggatatcgca aaaggggcca acgtcatcaa
     4621 atatggcctg ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc
     4681 ccacaatacg cgcacgaatc tgagcgatct ggatcagtat cgctatcaac ctgattttca
     4741 ggatctgcct gcgcaagcgg cagatcctga agtgcagatc tatcgtcgcg ctaacggcga
     4801 tgtcggggtg cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca acggcatcgc
     4861 gcggcaaatc cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta ccgacggcgt
     4921 gttcctcttc agccacacct acggctgctc acaactgggc gacgatcaca tcaacacccg
     4981 caccatgctg caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg tgattggtct
     5041 gggctgtgaa aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata tcgatcctga
     5101 acgcgttcat ttcatgatct gccaacagca ggatgatgag atcgaagccg ggatcgagca
     5161 tttgcatcag ctgtataacg tgatgcgcaa cgataaacgc gagccaggca aactcagcga
     5221 actgaagttt ggtctggagt gcggtggttc tgacggtctt tctggtatta ctgctaaccc
     5281 gatgctgggg cgtttctctg actacgtgat tgctaacggc ggtactaccg tactgaccga
     5341 agtgccggag atgtttggcg cagagcagtt gctgatggac cattgccgcg acgaagcaac
     5401 gtttgaaaaa ctggtcacca tggtcaacga cttcaaacag tactttattg cccatgatca
     5461 gccgatctac gaaaacccat caccggggaa caaagcgggc ggtatcacca cgctggaaga
     5521 caaatcactt ggctgtaccc agaaagcggg ttccagtgtc gtggttgacg tgctgcgtta
     5581 cggcgagcgt ctgaaaacgc cagggctgaa cttgttaagt gcgccgggta acgatgccgt
     5641 agcgaccagc gccctggcgg gtgcgggctg ccatatggtg ctgttcagta ctggtcgcgg
     5701 cacgccatac ggtggatttg tgccgacggt gaaaatcgcc accaacagtg aactggcggc
     5761 gaagaaaaaa cactggatcg actttgacgc gggtcagctg atccacggta aagcgatgcc
     5821 gcagttgctg gaagaattta tcgacaccat cgttgagttt gccaacggta agcaaacctg
     5881 caacgagcgt aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa cgctataaag
     5941 tcgaaatgag ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt ttgcttttcc
     6001 atacctgcta aggatttatc atgaccgcgt attggctggc ccagggcgtg ggtgtcatcg
     6061 cctttctgat tggtatcaca acatttttca atcgtgacga acgtcgcttc aaaaagcagc
     6121 tttcggtcta tagcgccgtt attggcgtac atttttttct tctgggcacc tatcccgctg
     6181 gtgccagtgc catccttaat gccattcgta cattgattac cttacgcacg cgcagcttat
     6241 gggtaatggc gatttttatt gtgctgactg gcggaattgg cctcgcgaag ttccatcatc
     6301 ctgtcgaact attgccggtt atcggtacga ttgtcagtac ctgggcgctg ttccgctgta
     6361 aagggctcac catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg attcacaact
     6421 tctgggcggg gtcgataggc ggcacgatga ttgagggtag ttttctgctt atgaatggcc
     6481 tgaatatcat tcgtttctgg cggatgcaga aaaggggaat tgatccgttt aaagtagaga
     6541 aaacccctcc cgccatagac gaaaggggtt aaacaattaa ttacgcaggg cgctatttgc
     6601 cagacgatcg tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag ttgaagagtt
     6661 cagcgcggtt tcgcaagagt cctgcaatac gccgataata aagccgacgg caaccacctg
     6721 catggcgata tcgttcgaaa taccgaacat attacaggcc agtgggatca gcagcaaaga
     6781 accccccgcc acgccggatg cgccacaggc acacagagaa gccactacgc tcaacagcag
     6841 cgctgtgggc agatcgaccg gaatacccag cgtattaacc gcagccagcg tcaacacggt
     6901 aatggtgatt gctgcgcccg ccatattgat ggtggctccc agcggaatag aaactgaata
     6961 ggtatcgcga tccagattca gcttttcaca cagcgccata ttcaccggaa tgttcgctgc
     7021 agagctgcgg gtgaagaagg catacacacc gctttcgcgc aggcacagca gcaccagcgg
     7081 gaacgggtta cgacgaattt tccaccacac cagcaatggg ttaaccacca gcgccaccag
     7141 taacatacag ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg tggagaaacc
     7201 ggtggttgcc agggtagaag aaaccagccc aaaaataccg atcggtgcga agcgaatgac
     7261 cagtttcacc ataaaggtaa cggcattcga catatcattg accaggtttt tggtggtctc
     7321 gttaccgtga cgcagtgcga agccgaggcc gatcgcccac accagaatcc cgatgtagtt
     7381 acctttcagc agggcgtcga tggggttgga aaccatgctc attaccagtc cgcgcatcac
     7441 ttcgacaatg cctgacggcg gcgaaatatc acccgcgcta ctggacaagt gcagggtaga
     7501 agggaaggca aagctgaaga ctactgcggc cagagctgca gagaaggtgc ccagcagata
     7561 gaggaacaaa ataggacgga tattggtttt ctgcccgtgc tggtggttag caatagatgc
     7621 cattaccagc atcaacacca ggatgggggc aacagctttc agtgcgccga cgaacaaagt
     7681 acctaacaga ccaacagctt ccgccgcggg ttttgagatc catgccagaa gaatccccag
     7741 aacaaggccg accaggattt gttttaccag gctgccatga gccagacgcc ggaatagccc
     7801 cggtgaacgt tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac atccctggtg
     7861 cgttctgtcg acgcatcttt caggatgtaa caaagtgttt gcaaggtcga gtataaggaa
     7921 agttacagct tcgggaagcg aaaaatgctg gattttaaag agaggtcatg ttttgcaact
     7981 taatggttgc atttatttaa cataaacaga gggaaggact ggctattaag gctgattaga
     8041 taacatatgg gcaaaactat ttggaatatg agaacggttg cgctccttta acgtgttatg
     8101 ccatataaag tcactggcgc agggagcgcg cagggggcgg ccaatcgccg ccgccccctg
     8161 cacccccagg ctctggcgaa caaaatcgcc gctgcgcggt tccctcagcc tatcccttgc
     8221 ggctaccggg tcgggcgcaa ggtaacgtcc ctgtaagatg cgccctcgcc ccggcctcca
     8281 gggaacgctt cggcgatttt gacgccacca acacccgtgc ggtctttttg taaagagcta
     8341 taagccgatt acataattaa tagctcacct ttgtctggaa tgagttatct ctaggaatgg
     8401 cttaatatac agcgaatcat tctccccgct gcttatcatg ccgataattc acccaggcgt
     8461 tgataataaa cgtcatcacc agaatgccaa acaccacgcc cagcgagacg gcgattggaa
     8521 tatggtagaa gtcgacaatc agcatcttga taccgataaa caccagaatc accgccaggc
     8581 catatttgag catcgagaaa cgctctgcta cgcccgccag caggaaatac atcgcacgca
     8641 ggccgaggat tgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg gtcacggcga
     8701 agatagccgg aatgctatcc acggcaaaaa tcacgtcgct caattccacc agaatcagta
     8761 ccagcatcag cggtgtggcg tacagcaatc cattcttacg cacaaagaaa tgctcgttgt
     8821 cgatggtgtc tgtcatgcgc aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa
     8881 tgcctgattc atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac
     8941 cgaagatata caggatccag tcgaactgcg aaatcagcca gctgcctgtg aagatcatga
     9001 tggtacgcag aacaatcgcc ccgagcacgc catacaccag cacgcgacgt tgtaatgccg
     9061 ccggaacgga gaaatagctg aacaacatca gccagacaaa gacgttatcg accgccagcg
     9121 atttctcaat cagataaccc gtgagaaagg ccagggcctg tggatcggca acggcgcgac
     9181 cttcggtttg caccagatac caccagaaag cggcgttaaa cagtaacgac agcgtcaccc
     9241 agaccagcga ccaggccgca gcctgtttca tggtcatggc atgtgcccca cgacgcccct
     9301 gcaacaacag gtcgatagcc agcataatgg cgacaacaac agcgaatccg ccccatagca
     9361 acggcgtgcc gacagtattc atagaagttc cttacacata aaaaaacggc caacgtcgga
     9421 agacgtcagc cgctgctttt tatgcataga cctcgccttc cggcaaggtc tcacttacaa
     9481 caaaaaagga atacgtctgc gtattccttt cgggttgccc ggtgaccgga tgtggttttt
     9541 cacacatcgt aatgacgatc gaccggcaat gaagttactc ccctttgcag gtaacaaagt
     9601 atgacagacc attcagtcag tcaatggtct gttacatacc ttttacactg ttttactttg
     9661 gcaagtatag ttttacgcta tctgccggaa aaatcacccc agtctggcgg cggatctcgg
     9721 tcagcagttt ggctgtgatg cgactgaccg ccagccctgg atgatccacc agatgctcat
     9781 ccaccagggt agcgaacagc tctgcttcgt agagcatggt attaatatgc tgcggctggg
     9841 tgagatcctg catctggctg ccacgcggca caaagcagac tttctggcat tcagacagtt
     9901 tttcaatcac cagcgatccc gcttcgccct gaatctcgct tcccaggaca gaatcactga
     9961 cctttgagtg ctgcaaggtg acgctgaaat caccgtaatc catcaccacc acgccgtggg
    10021 cgtcaacgcc gcttgccagc aaactcgccg ttgcctgcac gcttttcggc tcaccaaata
    10081 acgccaccgc cgacgccagg cagtaaaagc cgatatccat aattgaaccg ttagagaatg
    10141 ccggattaaa ggtgttggga ttctcaccgt ccaggtaacg ttgataccgc gaggaatact
    10201 ggcaatagtt gaaaaagact ttacgcagtt tgcctgcttt cggcagcgcc tggcgcaaca
    10261 aatgaaagtt cggcaggcag gcggttttaa atgcctcaaa cagcaccacc tgattttcgc
    10321 gcgcacaggc aatggcggca tccacttccg ccagattcga cgccagcggt ttttcgcaaa
    10381 tcacatgaat tttatggcta aggaaaagtt gtgtctggga aaagtgcagg gaattcgggc
    10441 tggcaatata caccgcgtca atggcatcgc tttccgccat cgcttccagc gacgtaaaca
    10501 gatgctcgac agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg cgggaatata
    10561 cggcggttaa cttgtattta ccgctctcat gggcggcctc gacgaactgg cgagtgatcc
    10621 agttcgtacc aatcacagcg aaacgtatca taacgcgtgg atactccata aagggattct
    10681 gtcgtcaatg tagcacgcct ttttcatctt cgacgtgcgc agatacgcat tattctttcg
    10741 ccggacagcc atgctcacta aacggcccaa tcatgaccga tggcccgaga tatttcggct
    10801 ggcgatggag gatttttgcg tcaatcaccg cgctgacacg agcgagtttc tcccgcagtt
    10861 gagtataaaa accatgcttc atattgaggt ctggcgcgtt aaacccgata gcgtcgatgc
    10921 cgtactgttt tgccagccag atggcgcgtt ggttatgaaa ttcttgcgag ataatggtga
    10981 tatggttttc cccaaacacc tttttggcac gcactaccga atccagcgtt gagaatccgg
    11041 catagtcgca aaagatcact ttcgccggta cgcctttagc gatcaaagcc tgctgcatac
    11101 ctgatgcttc atcgtaattt ttacgtccgt tatcgccact caccagtagc catttcactt
    11161 ttccggcgtg gtacaacgct gctgcggtat caatacgtcg ggtaaagtag cgattacccg
    11221 gcctcgcccc cagcaataaa cccacgttac gcgccgggac ggcgttgaca tcgccccagg
    11281 tcagctgctt gctggcgttg accataacgc gatcggcgat aaaaatggtc gctcctgcca
    11341 caagtaacag taagcaggca atctttagag tccggcgaga aaaacaaata cggagaagaa
    11401 ggcgggcaaa tgcgcgcagc atggtcgtta tccttaacga aaggggggag gtcgatttta
    11461 ggcgctaaag aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc
    11521 gacaactgcg ttagccacag ccgggtgtcg aactcaagct ggtggtactg cggctccata
    11581 tggcagcaca attgatagaa cgctttgtta tgctctttct cctttaggtg cgccagctcg
    11641 tgcaccacga tcatgcgcaa aaacggttcc ggcgcgttgc gaaacacggt agcgacgcgg
    11701 atctctgctt ttgctttcag cttgccgccc tgcatacgag aaacagcggt atgtaacccg
    11761 agcgcgtttt ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca
    11821 ttacgcagaa actgattttt cagatcctga gtatattgcc agagagcttt atcggtggcg
    11881 tagtcgtgtg ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc
    11941 agtgttcgca cctgcgaaag cagctgctcg ggataaccct gaagataagt aagattgctc
    12001 ataaccgccc acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt
    12061 cgaaatttta tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga
    12121 cactacaacg ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcag
    12181 atgaatattt gctgcaacag ttggacgaca cagaaatccc cggcccggtg ttgatcctga
    12241 atgatgcctt tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg
    12301 actcatacat cagtgaactg gcgacgcgcg agaatttacg cctaaacggg attgatgagt
    12361 cgagcgtgaa gtttctcgac agcaccgccg actacccgca acagccgggt gtggtgctga
    12421 tcaaagtgcc gaaaacactg gcattgctgg aacagcaact gcgtgcgctg cgcaaagtgg
    12481 tcacgtcgga tacacgtatt attgccggtg ccaaagcccg tgacattcac acttccacgc
    12541 tggaactgtt cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc
    12601 gcctgattaa ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag accgttagct
    12661 ggaagctgga aggtactgac tggactatcc ataaccatgc gaatgtcttc tcccgcaccg
    12721 ggcttgatat cggcgcgcgc ttctttatgc aacatctgcc agagaatctc gaaggggaga
    12781 ttgtcgatct cggttgcggt aatggcgtta ttggtctgac gctgcttgat aaaaacccgc
    12841 aggcgaaagt ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg
    12901 ttgaaaccaa catgccagag gcgttggatc gcagcgagtt tatgatcaac aacgcgctct
    12961 ccggcgtgga gcctttccgc tttaatgctg tgctctgcaa ccctccgttt caccaacaac
    13021 atgcgctgac cgataacgtc gcctgggaga tgttccacca cgcccgccgc tgcctgaaaa
    13081 tcaacggcga gctgtatatc gttgccaacc gccatctgga ttacttccat aaactgaaga
    13141 aaattttcgg caactgcacc actattgcga cgaataataa atttgtggtg ctgaaagcgg
    13201 tgaagctggg acgtcgtcgg taagattggg agttacctgc cggatgcggc gtaaacgcct
    13261 tatccggcct acgttcagca cgttaccccc aggccggata agacgcggca agcgtcgcat
    13321 ccggcaatgt ctgctcagtt ctggaatcgc gcttccataa aacaaaaaat ccctgtcacc
    13381 atcctctaca ttctcttgtt tagcgttttt ctacgtttat tcttccgtca cacagataaa
    13441 ttcatccgtt gccaatctgt caacggatgt tatcatattg ccaatttgtc aacggacgtg
    13501 atgaagaatg cacctgataa ctcaaaaagc attgaaagat gctgcggaaa aatacccgca
    13561 acataaaacg gagttggtgg ctctggggaa cacgattgct aagggatatt tcaaaaaacc
    13621 tgagtcatta aaagcagtat tcccatctct ggataacttc aaatatctgg ataagcatta
    13681 tgttttcaat gttgggggca atgaattacg tgttgtagca atggtctttt ttgaatcgca
    13741 aaagtgctac atacgtgaag ttatgacgca taaagaatac gatttcttta ccgctgttca
    13801 tcgtactaag gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta
    13861 actgctgtgg caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg
    13921 gaactggtag atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt
    13981 gccaaaataa ccgcgtggga agaatcagcg cccgaatttg cggaatttaa tgccatggct
    14041 caagccatgc ctggcggtat cgccgtgatt cgtaccctta tggatcaata tggtttaacc
    14101 ctttccgatc tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag
    14161 aggaaattaa cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc
    14221 ttgtttattg attaacgcga cgtgccggat gcgttgcatc cggcgcttca aataacgatt
    14281 taaatctcca gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata
    14341 gccacatcgc agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc
    14401 agcgcgcggt ttggctcttg ccctgcgcag atcaccacat tgtccactgc taatacctgc
    14461 gtttcgccgt tgatcaccac atgcagcccg tcatcgtcaa tcttctgata acttacgcct
    14521 gggatcattt tcacaccccg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg
    14581 cctaacccct gtcccggttt gctggctttg cgctggagca tcacaatttg ccgtgggcta
    14641 cgcgggatct gcattccctg cgggcttaag ccaccagcct gttgtaggct gctgtcgatc
    14701 ccccactcat tacagaacct ggcgatattc tggctggtgg attcgcccgg ctgacttaaa
    14761 tacatcgccg tatcaaaacc aatcccgcca caaccgatga tggcaacctt gttgcctacc
    14821 ggcgctttgt cgcgcagtac atcgagataa ctcaatacct tcggatgatc gatcccgtcg
    14881 ataggcggaa cacgcggcac gatcccactg gcgaggatcg tttcatcgaa cgcctgtaac
    14941 tgatccgccg tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc gatcatccgg
    15001 cggtagtagc gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt ggcgatatta
    15061 aactgcccgc cgatctcgct gtgcgcatca aacaatgtta cctgatgccc acgcgccgcc
    15121 gcgttaatgg caaacgccag cccagcaggt ccagcgccga ccaccgccag attttttttc
    15181 tgcacggcgg gaaggattgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac
    15241 gaggtgactt tgccaacgaa gatctgatcg aggcaggcct ggttgcagcc aatacaagtg
    15301 ttgatctcat cggctcgccc tgattgcgct tttgacagca gttccgcatc cgcgagaaac
    15361 ggtcgcgcca tcgatactat atcggcatcg ccgcgcgaga gaatatcgtc ggcaacctgc
    15421 ggatcgttaa tccggttggt ggttaccagc tgcagcgaga cgtggccttt cagtttgcgc
    15481 gtgacccagc taaatgcgcc gcgcggcacg ggcgtggcga tggtcggaat ccgcgcttcg
    15541 tgccagccta ttccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagt
    15601 tctaccgttt cggcaaaagt gccgccgcct tcgaccaggt cgagcatcga caaccggtaa
    15661 ataataatga agtcgttgcc gacgcgttcg tgcaccgtac gcactacttc tacggcaaat
    15721 cgcatccggt tgcggtaatc gccgccccac tggtcactac gctgattggt gcgcagcgtc
    15781 agaaattcgt tgatcaaata cccttcagag cccatcactt cgacgccatc gtatccggcc
    15841 tcccgcgcca gttgcgcgca gcgggcgaaa tcgtcgatca gttgcaggat ctcttcatgg
    15901 gtgagttcgt gaggaacgaa acggttgatg ggggcctgca atgcggacgg tgcgaccaga
    15961 tgcggttggt agctgtagcg cccggtatgc aaaatttgca gggcgatttt gccgccttcc
    16021 tgatgtacgg cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc gttgagcatt
    16081 gcgccacctt ccatgccaac gcctattaaa tctggtgcaa tgccgccgct gacaatcagc
    16141 gccacgccgt gacgggcgcg ttcggcataa aacgcagcca gccgttcagc accgtccggg
    16201 tattcctcca gcccggtgtg cattgaaccc atcaacacgc ggttttttaa cgtggtaaaa
    16261 cctaaatcca gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat
    16321 aattgttatg tggtcggatg agttctaatt tagccgtcga tggagaaaag ggaaaagggg
    16381 agtgcggggt ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc cggatgcgac
    16441 gcttgccgtg ttttatccgg cttgggggat tgcacatgta gggcggataa ggcgttcacg
    16501 ccgcatccgc cagccgttgc aaattactgc ttacggaaaa aatcgttata caacatatac
    16561 aaatgagcgg cactccagga gaaatttggt gcgccttgct gcgcgcctgt cagcgggttg
    16621 taattctcct gaattgggcc atcggcggtt aatcctttgg cgtgcctgaa gaacgtatcc
    16681 gccagcttca gggcatcatc acgataaccg taacgctcca tccctttcag accaaaccag
    16741 aactgatcca cccatacgcg cccgcgccag tagatatcag cgccaaaagc cggattggtt
    16801 aacgccgccg ttcccagcgg aacaaaggtg ttgaactctt taggatcgag catcaccttc
    16861 accaccgcgt cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc
    16921 ggccctttac cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag tggtttatct
    16981 tcaatacgta cgtcatagta gaactgggta gtcgggtcga acatacaggt gttgatgtag
    17041 tccgcgagct gctgtgccaa ctggcgatag cgtttggcct cttctggctt accgaggatg
    17101 gttgccatct ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac
    17161 tcctgcaata gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc
    17221 accgtccagt cgctacgttt gccgccatta gcgacatatt tatccaactg ttctttgtcg
    17281 ataaaaccaa agacggcggc atcatcacgc cccgattccc acgacgcagc gacctgtgcc
    17341 ggaatttcca gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca
    17401 gactgcgtct cttctttatc gccttttttc accgtaaaca gcatctcgcc gctctcggtg
    17461 ttgtgggctt tgtcgcgggt cgcgccatat tccggcaccc cgttgccgtt atgatcgcgg
    17521 ttacgtaacc accagtcgtg ataggccacc agtttcgggt acatctctgc caaccaggct
    17581 ttatcctggg tgacgttgta tacttccatc accgaccagg cggcaaggct gggtttggta
    17641 ttacgttcgt tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt ccacgctatc
    17701 aggtcgggga caaagcccac atcctgcgga cgcacgctgt cgccaggctg gatctgccag
    17761 gagaagaccg cgcggatatt ctctttggcg atatccggat tgaaatgcgc catcgcaaac
    17821 gcctgcttcc aggtatccca cggccaggtc tgattgccgg agaaccagcg tccggtcacc
    17881 gacggcgtga cggtgttata attcaccgca ccgcccggtg agcgccagtt accgttgagc
    17941 gtttcgatcg ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg attggttaac
    18001 cctttcttca ggtattcttc ccagcgttgc tgcgaggcgg tgagataaaa cgccggacgc
    18061 gccagaatat cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg
    18121 gaataggtgg tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta
    18181 ccgttgattt cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag
    18241 gtcagcagat cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg
    18301 gcgctgattt tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt
    18361 ttcccttctt tcgcttccag tttttccagc agttcgccat cccacaccag atccagcggt
    18421 ttattgctga tgattttagt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc
    18481 atttcgacct gcacatcttt tgctgtcagt ttttgcacca gcgcgccggg aatactgtat
    18541 gcctccagcg tgaagttgac tttcttgccg tcttgccaga cagtcaggcg gtcgaaattg
    18601 ctggccataa agttgatgta ttcttccgtc agcagcgcaa cgcccggaaa gccgcccatg
    18661 gtgttcgggc cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta
    18721 aaacgctggt gatcgtcgta atcgtaatcc ttcatgtact gcggcgcacc agtacggtta
    18781 atcacgtttt tataattgtc ggcgttagcg gcatgagcgg aaaaacttat cagcagagcg
    18841 caggttactg gcgttaaaat agttttgatt ttcatgaaat tacagtcctt attaccagta
    18901 gaaatattgc atcaggaaga tttggtcgct gggatcgtta gaggcatttt tcatgtagaa
    18961 gcggctatca aaagccgtgg cgaaacgtcc gccgccatat tcgtaccaga taccaaactg
    19021 catgaaagag gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc
    19081 ggtggagaga taaacgccct ttaacgcttc atacatggcg ctcaccatca tcccgctttc
    19141 acttcccggt acgtccagcc cgtcggcacg tccggtatgg tgccaggcgc gagcggcaaa
    19201 gttaggggta aacaggcccg tgagatagag ttgtcctgtg ccgtcggtaa tttccagacc
    19261 attcatccag gtgatgcctt cggcaaggtc atactggatg taaccgttca tccattgccg
    19321 gataggtgta tttgtcgtcg tagcgatcgt acttgccaaa atgtagccag gctttgcttt
    19381 cgtcatgatg tccggcaggt gtggcggtga cgctataacg aaagttgccg cttaagttct
    19441 gtaccttcag cgcggtcata atgtcgcggg tattgggaat gacgtagccc aaatcgggtg
    19501 tgaagtcgcc ccaccattgc aggtcatcca gcgaggaatc ctgacgcaga ctgagcatga
    19561 tttcggtgcc atcattggta cgataaccgc catagaaacg gttaatccca ccttcgaaac
    19621 caccccagct atggtcaggc acctaggcat taccgtcgtg gtcggcctgc acggtccagc
    19681 cttcgccgta aatcaggccg aaccaggagc cgtacttcat ctccaggcca ccttcaatat
    19741 aggtgccgtc gctgtattta tgtttattct caccctctaa atccatatat ccaccaagcc
    19801 ctaattcgcc gtaaaaacga aatgaaggag aagatgtcga ggtaattgcg ggtgtttttg
    19861 tgtcgtcatg ttctgccgga cgtgcttctt ctcctgccgc agaaaaggcg tagcaaaacg
    19921 gcagagcaag taatgctctg catggtgcag tgattaattt cattgtgtta atccatgagc
    19981 taaagaaaaa gtacagcctt ttatcgttgt aattataaaa atataaaggc agaaactatt
    20041 ttccctctcc tcatccagga agagggaaaa tgcctgctca ttttttatta gtctgcttaa
    20101 tcaacattgc tggctggcgt cgcttcaata tggcgctctt cagccgttaa tccctttata
    20161 tatttactgt atttccacca cgcaaagccg aggaagataa caataccgcc gacgttataa
    20221 aaaatgatgg tcagaatatt cgcgccagtc gggaatgtcg aagcgacaaa ccctacggca
    20281 aatatcgcaa tcagcattga aacaacgata ataccggtgc ggcgggagcc catacggaaa
    20341 tcgcgtggca ggtgatctaa tttggcgcgt aaattcaggt aagccagcat gatgaataac
    20401 ggcggaagca tggacgctgc ggcggtcata ttaataatgg tattcatcag atcctgcacg
    20461 gtattggaac cgagcatcgg gataatcatc agcgggatga cgatcagaaa ctggatccat
    20521 gctgcgcgcg ccggaacgcc gttttcgtta agttcgacgg ttttcttacc aaagatgcct
    20581 tccgggattt cggagaagaa aattttcacc ggcgttgcgg tccacatcag cagggaaccg
    20641 aacattgcgg taaaggacac cagaccgaca aagcgattca tcaacgcttc cggcagacca
    20701 aaatacgccg ccatgccgtg gaatacctgc accgatccgc cggtaaattt caactctttg
    20761 ctgctgacga agacgttaat cagcaccgag gagacggaat acagtacgcc gataaaaatc
    20821 ccggcgagga tgatcacttt aacgaacgat ttcgaaccgc ctttgacgtc gttaacgtac
    20881 accgcgacgg actccgcgcc acctgcggcc ataaagatcc aggtggtaac gccgaggaac
    20941 gcccagttga agttcgggat catcgcgtca acggtgatcg gatcggcagg ctgtacgtcg
    21001 ccaaccagtg ccgtacctgc cagtaaaatg taggagagcg ttaacagcag catcagcgtt
    21061 gaagtgacgg aggtaattgg ccccagcatt ttcgccccgt tggtggaaac ccaggtggag
    21121 aaggcgaaca gcaccatact gataacggtg gtggcaaccg gcgtcataat atattcgtag
    21181 ccgaggaagg catacgaagc ataagcaata acgcgcggca acagcgaggt aaaaaagaac
    21241 agatttacga accagtaagt gtaggcagta ataaatgccc aacgtccgcc cagcgaactt
    21301 tttacccacg cgtagacacc ggcttctgag tttttattta acgaaacaaa ttctgcgatg
    21361 atcagacaga agggaataaa ataaaaaatc gtcgcgagga aaaacatcgg tgccgaggcc
    21421 agtccaagct caatattatt attgataacg ttgttaaagc tgtaaacggc ggcaaaagtc
    21481 agcgagagca agccgaattt gccgattgta ttacgtttgg tatcagacat aacacatctc
    21541 cgaaaatgtg tagggtagcg gtggcggcaa actccttttg ccgccgtagt tgttatttgt
    21601 tatggaaata accatcttcg atggtgactt tgagaaccac ttttttgacc gcgttattgc
    21661 agataaaccg atacgcttca tggatatcgc aaataacgat ttgcccttcg tggacctcta
    21721 cggtttctcc gcagcctttt aaatattcac ggtcagtttc atcacgataa tattccacta
    21781 cctgtaatgt ctctttcggc gcatattcaa ttttttgctg cccttgcagg tagtaatgca
    21841 cttcaaaata gcgtcgatgc ccggtaaata gcgcatcggt cgcggagtca ttttccacac
    21901 ggtaggtcaa tgaatcaccg atggagtggg cgacgccagg ctgaatgttg tcgatatttt
    21961 caatcgcttc aacgcagcgt tgccacttct tgctagaggc gtaaatctgg cggaactgtt
    22021 ctaagttatc gatgatcctc attgcttatt ctcgctgtgc aaattcgtgg aaaagaaccc
    22081 tgcgccgaac tcatacgacg ccaggctttg cgcggtagct tctccgccag aaaccggcag
    22141 caacgtaaag ccgtagctga agtcacggaa ccagacgcgc caggagtcca gcacctcgct
    22201 gccccaggag ttggagccga ggccaagtag ctggtggtcg aggttcaggg tgatgtcatc
    22261 actgcgctgc agctcgttac agtgctgggc agcgtggatg ttttcctggg tatagtgcca
    22321 ggcgctgaag ttaattgggc gctgcggaac caccagcaga ccgttgtcgt ggcggttagt
    22381 cagtgccgtc cagcggacat gctgacggtt gccgttgttc tgcgggaagg gatagttctc
    22441 gaacatggca tcgacggtgc tgcgccagat atcgatgatg ttagcctgct ggctgtcggc
    22501 gtagttttcg cccggtccac gaccgtaata cgccacctga tcgtattcgc cgttaatccc
    22561 catggtgaaa ccgatgcacg gaatgatgtg cggatagtcg ccgtaacgct cgccggaaag
    22621 cgccacgtta acctggccat cggcagcgat gcgccagatg taggtacagc gcatcccgaa
    22681 gtcaaacacc ggcggtgcaa taactgtgcg gctgatgatc aacacttcac catcgctctg
    22741 ttctacggca aagtcgcgca gatgttcctg catgatctgc aaatgattcg gctgccacaa
    22801 cccttcgtac tcctgcttgt ggttgtcgat catcggcttg aagaagttga tctttggctc
    22861 gcgggtcagc agcgactcgc catttacctg ccaggatgtc ggtttgccac tcatttttga
    22921 gaaggtgatc gcgaagttgt agccgcgaac ggtgcagctc aaacgatcgt cttccagcgt
    22981 cagcggacgc gcattatttg gtgcgaaagg cactggctgc gcggtgtttt ccttcagcgg
    23041 gaactgataa gtggcgatag aatgcccggc ttcgctgtag cgggtgcggg aatctttggt
    23101 caccgtaatg ttgaggaacg cttcgcgggc gtccagctgc ggcagcgtga tctgcaaggg
    23161 ggcttcgctg ttcggcgcaa cgtcgcgcag tttaatctgc tgcgtcgcga gcgtttcacc
    23221 ttcggcgcgc acctctgcgt gcagggtgta gtcatcaagc gtggtaaacc acagtttatt
    23281 ttcgactttc aattcgccgc gagtcagcgc gtggattttt accggcgcga taacctgttt
    23341 gtactctttc aggcccgatc ccggcgtctg atcggaatag atcaaaccat caagacagaa
    23401 gttatagttg ttgggatagt cgccgtagtc gccgccgaat ttataccaga cattgccgtg
    23461 gtcgtcctgt gcctggatcc cgtggtcgca ccactcccag acataatgac cctgaatgca
    23521 atcgtgctta tagaagacgt tctggtactc cgtcagcccg cccggtccgt tccccatcgc
    23581 atgagcatat tcacagatga tgcgcggctt cggatgcggg tattcaccaa actcattcat
    23641 cagcggcacg cgggtgtaca tggtgggaat aatatcgacc acttcagcat cgcgatcttc
    23701 ttcgtaatgc accagtcgcg tgtcatccag cgctttcgcc gcatggtaca tcgcgcggat
    23761 gttacagcca tagccggatt cattgcccag cgaccagatg atgatcgacg gatggttttt
    23821 ctgtgcgtgg atatggcgaa caatgcgctc gacgtagact ttttcccact gcggatcgtc
    23881 ggtaatacgg ctgatatcgc cgacattagc aaagccgtgc gattcgacgt cggtttccgc
    23941 catcacaaac aggccgtaga tatcacacaa ttcgtaaaaa cgcggatcgt tcgggtagta
    24001 agcggtacgc acggagttga tattgtgctg cttcatcaac tggagatctt tctcgacgcg
    24061 atccattcca acggcgcggc ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag
    24121 catcacataa cggttattga tccagaacag accgtcgcgc actttgatat cacggaagcc
    24181 aacgcgttgt ggcaccactt ccagaacgtt gccgttggcg tctttcagcg tcatgaccag
    24241 atggtaaaga taaggggatt ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa
    24301 gctggcgctg gtcagttttt caattgccaa atgatcaatg gcgctgctgt gcaccacgcg
    24361 ttcgccatca aacagggtat attccagcgt cgtgacgaca ggggaggcgg cgagattttc
    24421 cagcaccact tcgcaggaaa gcgtggcatc gcaataggct tcgtcaaagt cggtacgcac
    24481 agtgaaatcg ttaatatgcg ttaggtgttt tccgaccaga taaacatcgc ggaagatccc
    24541 cgccgaccac cacatatcct ggtcttccac gtaggtagag tccgcccact gcatcacgcg
    24601 cacacacaac aggttgtcgc cggttttaac catcgcgctg atgtcaaact ctgcggtcag
    24661 gcgactgccc ttgctgaaac ccacatactg accgttaacg tagacttcaa aataggtttc
    24721 gacgccgtca aatttaatca gcgtctgttt accctgccag ccgtcgctga gggtgaaaat
    24781 acgttgatag gcaccggttg ggttatcgct ggggacaaac ggcacatcga tggggaacgg
    24841 aaaaccttcg tcggtatatt gcagtttgcc gtgaccttcc atttgccaca tggcggggac
    24901 ggtaatatgc ccccagtcag ccattaactc agaggtgaag gcttctggta cttgcagcgg
    24961 atggtcaaaa aagtggaaat tccactgacc gcttaagggc agaaacaggc tgctggtttc
    25021 gcgggcaaag gtacgcgctt gcgcaacaga atcatatgaa aaaaagtacg cacgcggcgc
    25081 aagtcggttt tcgtgggtga gctgaatgtt ttcccagcga ttcatagggc ctcccggtag
    25141 agagaaatcg gaaccattca tggtgatggt agtttgtgta attgctgggg gtttagtaaa
    25201 atttttacta aaagtagcag gtaaatgcat ttactttaac tggatcacga aaataaacgg
    25261 cagcactgtc cggcaaaaat gattgccgga cagtgtgtaa ggggatttaa cgggtcgtgc
    25321 cgcgcagttt taatttgctg ggaacgaaga ctagcagcgg cagcgcgcga ccatcgcggg
    25381 ctttttcata caccaggtta acgccctgac ttcccatcat ctcggaatgg atgcgtacgg
    25441 tggagagcgg cggaaaggta aatcgcgcgg tggggatatc gttaacgctg ataagcgaaa
    25501 tatcttgtgg gatgttcagg ccacgttcat gaattgcccg cagtacgccg atagcaatgg
    25561 aatcggaagc aacaaacagt gccttcggat agtcttcccg cgccagcatt tgttttgcca
    25621 gttcataacc cgacgaactg gaaaaaccgc cgcgccagat atcctcttcg cgtaccactt
    25681 gtttcagtcg gccatattcc gcaaaggcga cctcacgaat atccgccttg ccaggctcat
    25741 cttcaccgcc aataaaacca atacgattaa cgccctggtt gatatagaag tcgatgattt
    25801 ctttactgat gcgtgccaga tcgatatcca ccgcatcgta accgctgccg ggttcgtgaa
    25861 agtcgataaa acagatattg tcggtcaacg cgctggcagc ggcgcgcagg gcgggcgtgg
    25921 gtttgccgac aattaaaata ccggtgacgt ttttaatgtc tggtaatccg ttgtgttcat
    25981 aacagttgtt cagttcgatc gccagctttt cgcactgagt ttcaatgccg tggcggatcg
    26041 ccagatagta aggatcgttg atctccagct cctgctggta gctgtagata gccagaatat
    26101 ggtgttggtt gactgcgcct gtctgtagtt tacgggcgct actggtcttg tactccagct
    26161 tttcggcgat ctcgagaatg cgatgtttcg tctcttcttt cacattcaat gtcggatcgt
    26221 catttaagac cctggatact gtcgccaggg atacgccagc ttcgattgcg atgtctttta
    26281 atgttgccat gtttaccttt tttactttcc ctgatgtgcg cccccattgt agaggaagtg
    26341 cttatcgggt cacctgtttt cagtaaataa agcgcgtttc tttgacgctg taacggtaac
    26401 acagcggctt aggaatgtga tcacgcctgc attctccatt tttagcctac aggattgaga
    26461 aaaacttttg ctatagttta gtaaaatttt acccgaaggg aggaagcaat ggaaaccgtg
    26521 gcttacgctg attttgcacg tctggaaatg cgcgtcggaa agattgtgga agtgaaacgc
    26581 catgaaaacg ccgacaagct gtacatcgta caggttgatg tggggaaaaa acactgcaaa
    26641 ccgtgaccag cttggtgccg tactacagcg aagaggagct gatggggaaa acggtggtgg
    26701 tcttatgcaa tctgcaaaaa gcgaaaatgc gtggtgaaac gtcggaatgc atgctgttgt
    26761 gtgcggaaac cgatgacggc agtgaaagcg tgttgttaac gccggagcgg atgatgcctg
    26821 cgggcgtacg cgtcgtataa aaagatcgga tggcgacgtc gtatcgccat ccgatttgat
    26881 attacgcttc ttcgacactt actcgcatgg ccgccagcgc cttacgcgcc gctttgatca
    26941 ccagttcaca ctgttcaatg gtcagtgtca gtggcggttc aatgcggatc gttttggcgt
    27001 tattgagcgt tccggccacc agtacgcgct ggcggaacat ctcgctggca aagttatagc
    27061 cgatttcgtt atcaacaaac tcaatcgcca tcaacatccc tttaccacgc gcttcctgta
    27121 ccagatcggg atattcccgc gccagttgac ggaaaccgtc cagcaacaca ttgatggtcg
    27181 ccagcgccgc cgcacaggcc agcgggttgc cgccaaaggt ggtggtatgc aggaatgggt
    27241 tgtcgaacag aactgaaaac acctcttcag tggcgatggt cgcgccaatc ggcatcacgc
    27301 cgccgccgag ggcttttgcc agacacagaa tgtccggctg aacgttctca tgttcgcagg
    27361 caaacatctt gcccgtgcgc cccatgcccg tttgtacttc atcgaggatc atcagtgcgc
    27421 cgaactcatc gcacagctta cgcacggcgg tgagatagcc cggcggcggc agaattacgc
    27481 caccttcgcc ctgaatcggt tcgaggatca ccgcagccac atcatcaccg gtttttttgc
    27541 actcattaag agccgtgcgc atggcttcga tattgccaaa cggcacatga cggaaccctg
    27601 gcagcagtgg cataaacggt ttgcggaagg tcgatttcgc cgtggccgac agcgcgccaa
    27661 gtgatttacc gtggaacgcg ccgctggtgg caataaaagt aaatttgccg cgcggcgact
    27721 gataagcctt cgccagcttc agcgccgctt caacggactc ggtgccgcta ttacagaaga
    27781 agctgtattt cagtttaccg ggcgttagcg cagcaagggt tttcgccagc atagcccgta
    27841 gcggatcgag tagctcctgg ctgtgcagtg gttgtttcgc aagttgattc tgtacggcgg
    27901 aaaccacaac tggattacgg tgccccacgt tgaaaattcc aaaacctccc aggcagtcga
    27961 taaactcctg tccctgggtg tcgacaagcg tatttaaacc tcccgcttgc cactctacgg
    28021 ctccgtaatc cccgccggcg gtaacagatt tgcgatactc taaaaacccc ggattgacat
    28081 gctctttgaa gtattcaatc acctctcggt taagtgcttt catctcctca tgatccagcg
    28141 ttcgcttctc aatgagattc agggcgtggg cgctgcacgc taaagccgat gcgctcgaag
    28201 gtaacctgtt caaaatatgc tccggaggct cgcgtatcac atgataccga tttaagtatt
    28261 gcagggattg cgccactccg gctgcgatcg cgaaaatcgg gataaacaca aatgcggtta
    28321 tcatttgtgc aatatttaat gctatccgtt aacatttgtt gttattttgc acggttttgt
    28381 cgggtatgga tttgatgatt gctgcgcaaa tgcacgtttt cagggcaata attgcctgat
    28441 ttttgggcaa gataattaac gttttcgtat agtaacagag tgaaagtatt ttaaatcaaa
    28501 gaattagatt tgtgctttaa tcgcaaattg cgatctaaat caaattaatc ggttaaagat
    28561 aaccgcagcg gggccgacat aaactctgac aagaagttaa caaccatata acctgcacag
    28621 gacgcgaaca tgtcttctca tccgtatgtc acccagcaaa ataccccgct ggcggacgat
    28681 accactctga tgttcactac cgatctgcaa agctatatca ctcatgctaa tgacactttt
    28741 gtgcaggtga gcggctttac cttgcaagag ttacaagggc agccgcacaa catggtgcgt
    28801 cacccggata tgccaaaagc ggcgtttgcg gatatgtggt tcaccctgaa aaaaggggag
    28861 ccctggagcg gcatcgtgaa aaatcgccgc aaaaatggcg accattattg ggtgcgggcc
    28921 aatgcggtac cgatggtgcg cgagggaaaa atcagtggct atatgtcgat tcgtacccgg
    28981 gcgacggatg aagagattgc ggcggtggag ccgctgtaca aagcgttgaa ctccggacgt
    29041 accagtaagc gtattcataa aggcctggtg gtgcgtaaag gctggctggg taaactgcct
    29101 tcattaccgc ttcgctggcg gacgcgtgga gtgatgaccc tgatgtttat cttgctggcg
    29161 gccatgcttt ggtttgttgc tgccccggtg gtgacgtatt tcctctgtgt gttagtggta
    29221 ttgttggcaa gcgcttgttt tgaatggcag attgtgcgcc cgatagaaaa tgtcgcccgt
    29281 caggcactga aggtggcgac cggagagcgt aatagtgttg agcacctgaa tcgcagcgat
    29341 gagctggggc tgacattacg cgcggtaggg cagcttggcc tgatgtgccg ttggctaatt
    29401 aacgatgtct caagccaggt gtccagtgtc agaaatggca gtgagacgct ggcgaaaggc
    29461 accgatgaac tgaacgaaca tacccagcag acagttgata acgttcagca aacggtggcg
    29521 accatgaacc aaatggcggc gtcggtgaaa cagaactctg ccacggcgtc ggctgccgat
    29581 aaactttcta tcaccgccag taatgcggca gtgcagggtg gggaggcgat gaccacggtg
    29641 atcaagacaa tggacgatat cgccgacagt acccagcgca ttggcaccat tacttcgctg
    29701 attaacgata ttgcgtttca gaccaatatt ctggccctga atgcggcggt ggaagcggcg
    29761 cgtgccggcg aacagggcaa aggttttgca gtggtggcag gggaagtgcg tcatttagcc
    29821 agccgcagcg ctaatgctgc caacgatatt cgcaagctga ttgatgccag tgctgataag
    29881 gtgcaatccg gttcgcagca ggtacacgcc gccggacgga cgatggaaga tattgtggca
    29941 caggtgaaaa acgtcaccca gttgatcgcc cagattagcc attcaacgct ggaacaggcc
    30001 gatgggcttt ccagcctgac ccgtgcagtg gatgagctta acctgatcac ccagaaaaat
    30061 gccgagctgg tggaagagag tgcgcaggtg tcggcgatgg tgaaacaccg cgccagccga
    30121 ctggaagacg cggtgacggt gctgcattaa tcggtatgcc ggatccggcg gttggagcac
    30181 aatacctgat gcgatgctgg cgcatcttat caggcctgcg gggtgtgtag cgggtgtagg
    30241 ccggataagg cgcgtcaagc gtcgcatccg gcaattgcac cgcgccactg gcggatgcgg
    30301 cgattaatcc agttgcgtaa tatcaaaagc ggcgcggtcg atcaccgcaa tgatcttttt
    30361 tatttgtgca tcactgatat cgctctggtt gacgcgtaaa tccagcaccg ctttaaaatt
    30421 atccagcgcc cgcttcattt gcgggttctg gcgcagcgcc gcgccaacgc aacgcgcttt
    30481 gatgcgttct tcaatcatct ccacctgttc gcggttttct tccagccact gcgcgccttg
    30541 ttcggtcagc gcaatctgct tcttaccacc ttcctcttcg cggatggtaa tcagcgactg
    30601 ctcctgcaga aaatccagcg tcgggtagat gacgcccggg cttggggtgt aattcccctg
    30661 ggttagattc tcaatcgctt taatcaattc gtaaccgtgg ctgtcatcgc gcgagagaat
    30721 atccagaatc accagacgca attcaccgtg accaaagaaa cgttgccgac gaccgccgcc
    30781 gcgaccgtgg cgaccaccgc aacattgacc gtgttcatgc tggtgaccgt gtccgcagtg
    30841 ctcgtgttct gacttctcac ctttgcagca gccctcatgg cgtggctggc cttcatgttt
    30901 acaacaccct tcgtgatgat ggctcatatc agcctccttt tgcttgatta gatatatcta
    30961 atttatatct gatgtgagca ttactgcaag tacaaaaatg atttttaaac gtggaagcac
    31021 ctcacatcta ggccggataa gacgttcact ccgcatccgg cagcattgct atggcactga
    31081 atgccttcac atacttcaga catatcagtt ctacaaatcg cttgcattta tcatgattaa
    31141 caatcattat catttgcgaa ttttatttag atatatctga ttaccatcac gaaggcgata
    31201 acaatgaata acaccccccg ctacccgcag cgcgttcgca atgatctgcg cttccgtgaa
    31261 ctgactgtgc tacgcgttga gcgcatcagc gccggttttc agcgcattgt ccttggcggc
    31321 gaggcgttgg acggttttac atcgcgcggc tttgacgatc acagcaaact cttctttcct
    31381 caacctgacg ctcactttgt gccgccaacg gtaacggaag agggcatcgt ctggccggaa
    31441 ggaccacgcc caccgtcgcg tgactatacg ccactgtatg acgaactacg ccatgaactg
    31501 gcgatcgatt tctttattca cgacggtggg gtcgccagcg gctgggcgat aaacttacgg
    31561 tggcaggtcc gcgcggttcg ctggtggtgc cggaagatta cgcgtatcag ctgtatgtct
    31621 gcgatgaatc cggaatgccc gcactgcgcc gccgcctgga aacgttgagc aaacttgccg
    31681 ttaaaccgca agttagtgcg ctggttagcg tgcgggataa cgcctgtcag gattatctcg
    31741 cgcaccttga tggttttaat atcgaatggc tggcacatga tgagcaggcg gtagatgcgc
    31801 gtctggcgca gatgcaaatc cctgccagat gattacttca tctggataac cggcgaaggt
    31861 aaagtcgtta agaatttaag ccgccgcttt gaagcggaac agtatgaccc acagcgggta
    31921 cgtgcagcgg cttactggca cgcaaagtaa ctgatatata aaaagaaaaa ggctgacgat
    31981 ttctcgtcag cctttgctat atctggtggc ccctgctgga cttgaaccag cgaccaagcg
    32041 attatgagtc gcctgctcta accactgagc taaggggcca tggtagcgga ttataaagta
    32101 actccgtgtc gcaatccagc cattaccgcg cgcctgctgt ttttataaac aatgtatttt
    32161 caatccgtta tacttttcct ggtgatgtta gaaaggagta aatatggttg aggatatttt
    32221 ggctccaggg ttacgggtcg tgttttgcgg tatcaaccct gggctttcat ccgccgggac
    32281 tggttttccc tttgctcatc cggcaaatcg cttctggaag gtgatatatc aggccgggtt
    32341 taccgaccgt cagttgaagc cgcaggaggc acagcatctg ctggattatc gttgtggcgt
    32401 caccaaactg gtagaccgtc caacggtgca agccaatgaa gtttcaaagc aggagctgca
    32461 cgcaggtggg cgtaagctga ttgaaaaaat tgaagattat cagccgcagg cgttggcgat
    32521 tctgggcaaa caagcatatg aacagggatt cagccagcgc ggtgcacagt gggggaaaca
    32581 aacgctcacc attggttcga cgcagatttg ggtgctgcca aatcccagcg gtttaagtcg
    32641 cgtttcactg gagaaactgg ttgaagcgta tcgcgagctg gaccaggcgc tggtagtgcg
    32701 tgggcgataa aaaacgccac cga
//
LOCUS       DADMEQ010000035        32329 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.035, whole genome shotgun sequence.
ACCESSION   DADMEQ010000035 DADMEQ010000000
VERSION     DADMEQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.035"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="JNZ05_001665"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="JNZ05_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HAY8564934.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="JNZ05_001666"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="JNZ05_001666"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAY8564935.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="JNZ05_001667"
     CDS             complement(3736..4092)
                     /locus_tag="JNZ05_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY8564936.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="JNZ05_001668"
     CDS             complement(4096..4512)
                     /locus_tag="JNZ05_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAY8564937.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRSVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /locus_tag="JNZ05_001669"
     CDS             complement(4623..5336)
                     /locus_tag="JNZ05_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B acid phosphatase"
                     /protein_id="HAY8564938.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5699..6241
                     /locus_tag="JNZ05_001670"
     CDS             5699..6241
                     /locus_tag="JNZ05_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005059113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564939.1"
                     /translation="MVINFITRDGDDDMNISYVNSNKTTSLPVELDALNNKDISYAKD
                     FSYAKDFFLYIETQLKIAKDFCRPGEEVSSSIASKVFHAFIDLVNKIRGKKDFMYICT
                     LCCFAEEVKGDYSHYRTFLFDIGNQYKVKLIQSGKKEFSLTLEFNDTIIESQKVTGNK
                     AKHILEDIEIFYRNKPDTYY"
     gene            complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="JNZ05_001671"
     CDS             complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="JNZ05_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HAY8564940.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7910..8989)
                     /gene="alr"
                     /locus_tag="JNZ05_001672"
     CDS             complement(7910..8989)
                     /gene="alr"
                     /locus_tag="JNZ05_001672"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAY8564941.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9042..10457)
                     /locus_tag="JNZ05_001673"
     CDS             complement(9042..10457)
                     /locus_tag="JNZ05_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase DnaB"
                     /protein_id="HAY8564942.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10540..11523
                     /locus_tag="JNZ05_001674"
     CDS             10540..11523
                     /locus_tag="JNZ05_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HAY8564943.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="JNZ05_001675"
     CDS             complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="JNZ05_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HAY8564944.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="JNZ05_001676"
     CDS             complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="JNZ05_001676"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HAY8564945.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13465..14172)
                     /locus_tag="JNZ05_001677"
     CDS             complement(13465..14172)
                     /locus_tag="JNZ05_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HAY8564946.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(14196..14450)
                     /locus_tag="JNZ05_001678"
     CDS             complement(14196..14450)
                     /locus_tag="JNZ05_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564947.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFENKDATKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            14768..15283
                     /gene="zur"
                     /locus_tag="JNZ05_001679"
     CDS             14768..15283
                     /gene="zur"
                     /locus_tag="JNZ05_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HAY8564948.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15325..15534)
                     /locus_tag="JNZ05_001680"
     CDS             complement(15325..15534)
                     /locus_tag="JNZ05_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAY8564949.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15650..17029)
                     /gene="dinF"
                     /locus_tag="JNZ05_001681"
     CDS             complement(15650..17029)
                     /gene="dinF"
                     /locus_tag="JNZ05_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709876.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HAY8564950.1"
                     /translation="MPPGVAVCFSSLFIRLVCMAFLTSSDKALWHLALPMIFSNITVP
                     LLGLVDTAVIGHLDSPVYLGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQA
                     LARALVQPLLLALGAGALIALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPAS
                     LANLVLLGWLLGVQYARAPVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYA
                     TLLIGLLMVRKILKLRGISGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVL
                     GARLGSDIIAVNAVLMTLLTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACR
                     QSGIVALLFSVVYLLAGEHIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMF
                     IGATRAAEMRNSMAVAAAGFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHW
                     RNGTWFAAT"
     gene            complement(17048..17656)
                     /gene="lexA"
                     /locus_tag="JNZ05_001682"
     CDS             complement(17048..17656)
                     /gene="lexA"
                     /locus_tag="JNZ05_001682"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HAY8564951.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17766..18134)
                     /locus_tag="JNZ05_001683"
     CDS             complement(17766..18134)
                     /locus_tag="JNZ05_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAY8564952.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18305..20728
                     /gene="plsB"
                     /locus_tag="JNZ05_001684"
     CDS             18305..20728
                     /gene="plsB"
                     /locus_tag="JNZ05_001684"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HAY8564953.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20883..21755)
                     /gene="ubiA"
                     /locus_tag="JNZ05_001685"
     CDS             complement(20883..21755)
                     /gene="ubiA"
                     /locus_tag="JNZ05_001685"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HAY8564954.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21768..22265)
                     /gene="ubiC"
                     /locus_tag="JNZ05_001686"
     CDS             complement(21768..22265)
                     /gene="ubiC"
                     /locus_tag="JNZ05_001686"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HAY8564955.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLFDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22488..24068)
                     /locus_tag="JNZ05_001687"
                     /pseudo
     CDS             complement(22488..24068)
                     /locus_tag="JNZ05_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24297..25217)
                     /gene="malM"
                     /locus_tag="JNZ05_001688"
     CDS             complement(24297..25217)
                     /gene="malM"
                     /locus_tag="JNZ05_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HAY8564956.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKIQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLIIDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTMEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25369..26709)
                     /gene="lamB"
                     /locus_tag="JNZ05_001689"
     CDS             complement(25369..26709)
                     /gene="lamB"
                     /locus_tag="JNZ05_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HAY8564957.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26781..27896)
                     /gene="malK"
                     /locus_tag="JNZ05_001690"
     CDS             complement(26781..27896)
                     /gene="malK"
                     /locus_tag="JNZ05_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HAY8564958.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28261..29451
                     /gene="malE"
                     /locus_tag="JNZ05_001691"
     CDS             28261..29451
                     /gene="malE"
                     /locus_tag="JNZ05_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HAY8564959.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29605..31149
                     /gene="malF"
                     /locus_tag="JNZ05_001692"
                     /pseudo
     CDS             29605..31149
                     /gene="malF"
                     /locus_tag="JNZ05_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
     gene            31164..32054
                     /gene="malG"
                     /locus_tag="JNZ05_001693"
     CDS             31164..32054
                     /gene="malG"
                     /locus_tag="JNZ05_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HAY8564960.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8229 a         8289 c         7802 g         8009 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtgaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgcc tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acgctgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctcttatg ccaaagattt ctttttatac atagaaacac agttaaaaat
     5881 tgcaaaagat ttttgtagac ctggagaaga agtatcaagt tctattgcaa gtaaagtttt
     5941 tcacgcattt attgatttag ttaacaaaat caggggtaag aaagacttta tgtatatttg
     6001 cacgctttgc tgttttgccg aggaggttaa aggtgattat tctcattaca ggaccttttt
     6061 atttgatatt ggtaatcaat ataaggttaa acttatacaa agcggaaaaa aagagttctc
     6121 tttaacatta gaatttaacg atactataat tgaatctcag aaagtcacag gcaataaggc
     6181 aaagcatatt cttgaggata tagaaatatt ctatcgtaac aaacccgata cttattatta
     6241 aatttaataa tggttagatg tgcagaaatt attactccac acgaaaaaat agggaaagga
     6301 attccagata attgggataa acttaacaat aaaagaactg acaacgtttt attatctctc
     6361 acgtagaaca acggcatcaa aaaaatcatc tacaaagaaa tagtgaaagc gacgacagtt
     6421 taatttcact gcaggctggg taactccagc ctgctttcct gcattacatc accgcagcaa
     6481 acgcctttgc cacacgttgt acatttgccg tatttaaccc ggcgacacac atgcgaccgc
     6541 tggcgatgag atagacacca aattcttcac gtagtcggtc aacctgagcg gcacttaaac
     6601 cggtataact gaacatgccg cgctgattaa gcagataatc gaaattgcgt tctggcatct
     6661 ctgtgcttaa taccttcacc aattcctgac gcattgccag aatgcgagta cgcatctctt
     6721 ctacttccgc cagccagctg gctttcaatg cctcgtcatt cagcactgca gccaccacct
     6781 gcgcaccaaa attcggcggg ctggagtagt tgcggcgaac tgttgctttc aattgcccca
     6841 gtacgcggcc agcggcttcg gcatcttcac acagaacaga aagtccgccg acgcgctcgc
     6901 cgtaaaggga gaaaattttc gagaacgaat tgctcaccag agcgggtaat ccagcgctgg
     6961 caatggcgcg aatagcgtag gcatcctctt ccataccggc actaaatcct tgataggcaa
     7021 tatcgaggaa tgggataagc tcgcgggctt tgagaatttc aatcaccgca tcccactgat
     7081 cattagtgag atcggcaccc gttgggttgt ggcaacatgg atgcagcaac acaatactgc
     7141 gggcaggtaa tgttttcagc gtcgccaaca ggtcattaaa gcgcacgctg ttagtcactt
     7201 cgtcatacca ggggtaagta ctcacttcga atccagcccc ggcgaatatt gctacgtggt
     7261 tttcccaagt aggatcgctg acccagacgc ctgattccgg gaagtagcgt ttcaggaaat
     7321 ccgcgcccac tttcaatgcc cctgagccgc caagggtttg aatggttgct acgcgctgtt
     7381 gttgcagtac cggatggtcc gcaccaaaca gcagcggcgc aatggcatgg cgatagctgt
     7441 taagcccttc catcggtaaa taaagcgaag cgccatgggg ctgcgcattc aggcgcgctt
     7501 ccgcctccgc cacggctttc aattgtggaa taattccgtc ttcgttgtag tacagaccga
     7561 tacttaaatt cactttgtcg ctgcgagggt cttctttaaa acgctccata agcgtaagaa
     7621 tcgggtcgcc agcgtaggcg tcaacttttt gaaacacgcg atggttctcc aggtttacgg
     7681 gcaggtggtt aaaacacaat aaaccggaag aaggcgaaga tcgagtggat gttcaggagc
     7741 gaacggcaat tagcaacaga gtgagactaa tgataaacgt acatccgcca gaggcacgac
     7801 ctttataaga gcatgaaaga acatcaactt attgaatttt taggatttta ttggccggat
     7861 aaggcattca cgccgcatcc ggcacagaca atcaaatatt acagaacgat taatccacgt
     7921 atttcatcgc gacccttgaa gtcaggcgcg taataagttc gtaagcgctt actttcgtca
     7981 tttcagcgat acgttctacg ggcaaacctt cgccccataa aatgaccgga tccccggctt
     8041 tgtcctgcgc ctgtggacct aagtctacgc agatcatatc catcgccacg cgcccgacaa
     8101 tcggtacttc gcgaccgttc accagcactg gcgtaccgga cggcgcggcg cgcggataac
     8161 catcgccata gcccatcgcg actacgccaa gacgggtatc acgttcgctt acccaggttc
     8221 caccataacc aacaggctct ccggctttat gctcacgcac ggcaatcagg ctggaggtta
     8281 gtgacatcac tggctgacag ccaaaatcgg caccggtgga gcgatcttcc agcggcgaga
     8341 cgccataaag aatgatgccc gggcgcaccc agtcaaaatg cgactgtggc cacagcagaa
     8401 tgccacccga cgcggcaatg gaacgttgac caggtttgcc ttcgcaaaag gtattaaaga
     8461 tagcgagttg tttctcggtt gcgccacatt ttggttcatc cgcgcgcgca aaatggctga
     8521 cgatattcac cggctgacga acgtttttgc actgggtcag gcgatgataa aacgcctcag
     8581 cctgttccgg ccttacgccc agacggtgca taccggtatc gagtttcatc cagacggtaa
     8641 ccggctcgtc caagctagcc tcttccagcg cagccagctg ttcttcgtta tgcacggcgg
     8701 tatgaaaatg ttgcgcagaa atcgtcggca gatctctggc atcaaaaaag ccttcgagta
     8761 acagtacagg tttggtgatt ccccccgcac gcagtcgcag agcttcttcg agacgggcta
     8821 cgccaaaggc gtcagcatcg gggagcgttc gcgcggtctc aagaagaccg tgaccataag
     8881 cgttcgcttt caccaccgca accattttac tggcaggtgc cagttcacga agacgttgca
     8941 ggttgtgtcg cagagcgcgg cggttaatca caacagttgc cgcttgcatt tgtgttcctt
     9001 gataattgtt tgctttaatt acctaattca taaaataatt attattcgtc gtcgtactgc
     9061 ggccccgcat agttgtcgaa gcgcgaccat tgaccgttaa aggtcaggcg taccgtcccg
     9121 attgggccgt tacgttgttt accgataata atttccgcga tgccttttaa atcactgttt
     9181 tcgtgataca cctcatcacg atagataaac atgatcaagt ccgcatcctg ctcgatagag
     9241 ccagattcac gcaggtcgga gttgaccgga cgtttgtcgg cacgttgttc cagagaacgg
     9301 ttcaactggg acagcgccac caccggcacg ttcagttctt tcgccagtgc tttcagcgag
     9361 cgggagattt ctgcaatttc cagcgtacgg ttatcggaaa gcgccggtac gcgcatcagt
     9421 tgcaggtagt cgatcatgat aagcccgatg ccgccgtgtt cacgggcaat acggcgtgcg
     9481 cgggaacgca cttccgttgg cgtcaggccg gaagagtcat cgatatagat attgcgtttt
     9541 tcgagcaaaa tacccatggt gccggaaatg cgcgcccagt cttcatcatc gagctgcccg
     9601 gtacggattt tagtctggtc aacgcgcgac agcgacgcca gagaacgcat catgatctgt
     9661 tctgatggca tctccagcga gaagataagt actggtttat cctgcaacat tgccgcgttt
     9721 tcgacgaggt tcatcgcaaa tgttgtttta cccatcgacg gacgcgcggc gacgatgatc
     9781 aaatccgacg gctgcaagcc agcggttttt ttgttgagat cgtcataacc ggtgtttacc
     9841 ccggtaacgc catcgtgtgg ctgctgaaac aactgctcaa tacgcgccac ggttgcgtcg
     9901 agcacatcgg cgatgttctt cggcccttcg tctttgtttg cacgactttc ggcaatttta
     9961 aagacgcggg attcagcaag gtccagcaga tcttcgctgg tacgcccctg cggatcaaaa
    10021 ccggcttcag caatctcatt cgcaaccgag atcatctcac ggacaacggc acgttcacgc
    10081 acgatgtccg cataagcact gatgttcgcc gcacttggcg tattttttga cagctctgcc
    10141 agataagcaa aaccaccgac gctatcgagt tgcccctggc gttccaacga ttccgcaagg
    10201 gtaatcagat cgataggact accgctttcc tgcaaacgcg ccatttcagt aaagatatga
    10261 cggtgtgggc gggtgtaaaa atcgtctgct accacacgct cggctacatc atcccagcgt
    10321 tcgttatcca gcattaaacc gcccaacacc gactgctccg cttcgatcga gtgcggaggc
    10381 actttcagcc cggcaacttg tggatcgcgt tcgcggggtt cagcctgctg tttgttgaag
    10441 ggtttatttc ctgccatagt gaatggagtt accgaaataa agaatgggtc gaaactttac
    10501 catatgaagc agactctgac gatacgttct ggaggacaca tggcaacacg aattgaattt
    10561 cacaagcacg gtggcccgga agtacttcaa gccgtagagt tcactcctgc cgatccggcg
    10621 gagaatgaaa tccaggtcga aaataaagcc atcggcatca attttatcga cacgtatatc
    10681 cgcagcggcc tttacccgcc gccatcgcta cccagcggat taggtaccga agcggcaggc
    10741 atcgtgagta aagtcggcag tggtgtaaag catattaagg ctggcgatca tgtagtctat
    10801 gcgcagtcgg cgttaggcgc ttacagctct gtgcataaca ttaatgcgga taaagcggcg
    10861 attctgcctg cggcaatttc ttttgagcaa gctgcggcat ccttcctgaa aggcttaacg
    10921 gtttattatc tgctgcgcaa aacctatgaa attaaacccg atgaacagtt cctgttccac
    10981 gcagcggctg gcggcgttgg cttaattgcc tgccagtggg caaaagccct gggcgcgaaa
    11041 ctcatcggca ccgtaggaac cgcgcaaaaa gcgcagagcg cgctaaaagc cggcgcgtgg
    11101 caggttatta actatcgtga agagaatctg gtcgagcgat tgaaagagat caccggcggt
    11161 aagaaagtgc gcgtagtgta cgattccgtg ggcagagaca cctgggaacg gtcgctggat
    11221 tgcctgcaac gccgcggctt aatggtcagt tttggcaact catcaggtgc ggttaccggt
    11281 gtgaacttag gcattctcaa tcaaaaaggc tcgttgtatg tgacacgccc ttccctgcaa
    11341 ggctatatca ccacgcggga ggaattaacc gaggccagta atgaactgtt ctctttgatt
    11401 gccagcggtg tgattaaggt cgatgtcgcc gagcagcaga aatatccgct gaaggatgcg
    11461 cagcgtgcgc atgagattct ggaaagccgg gcgacgcaag gttccagcct gctgattcca
    11521 taaaagaaat agggcttcca cctgggaagc cctttctttt tatagttcgg ctgtatgtag
    11581 ggtacagcac gatgaatctg ttagaggcgc aataatgaca gatttgatta tcaattccta
    11641 ttttgttcta aggataaaac cttaggttgt gatcatccgc acaatccctt agtaacgcca
    11701 gcggtcataa cgctgatatt tcggcatttt tggtgcttta atcgccttaa taacccacac
    11761 caccgcaatc gccagtagta accacggcag caacttaatc atcaatgcca gcataccgcc
    11821 gaggaacata atggccgtcg ccacaaccag cgcggcgata atgcccagca acgaaacgcc
    11881 ggtgaccatc agcatgacaa aaaagccaat cacaaaaagt agttccagca tgatgctctc
    11941 ccaaatatga aatctcttgc tggcattaca agaatcatgc caaaaataat ctattgattt
    12001 aacagcaaaa cgccccgcga cggtgcgcag ggcgtggtga atttgactac tttttggtga
    12061 aaagttaacg cttatccgcc accagtttga gcgcgtgttc cagcacgtta atgtcagcac
    12121 ccgctttatg ggcattttca cttaaataac gccgccactg ccgcgcgcca ggaataccct
    12181 ggaacaaacc cagcatatgc cggataatat ggccgagata cgtcccctgg ctgagttcat
    12241 gctcaatgta cggatacatg gcacgcacta ctgccaccgg atcggcatcg gtatccgagg
    12301 aaccaaagat ctcccggtct accgccgcca gaatacccgg attctgatac gcctcgcgcc
    12361 cgaccatcac gccatccata tgttgcaaat gcgctttagc ttcttccagc gacttgatac
    12421 caccgttaat cgacattgtc agatgcggga agtcacgctt cagttgatac acacgcggat
    12481 aatcgagcgg cgggatttca cggttttctt tcgggcttaa ccccgaaagc caggctttac
    12541 gtgcgtggat gatgaacatc tcacactcgc ctttgccgga aacggtattg atgaaatcgc
    12601 agagaaattc atagctgtcc tggtcgtcga tgccaatacg cgttttcacc gtcaccggaa
    12661 tcgacaccac atcgcgcatc gctttcacgc agtcggcaac cagctgcgca ttacccatca
    12721 gacacgcacc aaacatgccg ttctgcaccc ggtcagacgg gcagccgaca ttcaggttga
    12781 tctcatcata tccgcgcgct tctgccagct ttgcacactg cgccagcgcc gccggatcgc
    12841 taccgcccag ttgcaacgct accggatgtt cttcttcact gtacgccaga taatcacctt
    12901 taccgtgaat aatcgcccct gtggtcacca tttcggtata aagcaacgta ttgcgggaaa
    12961 gcagacgcaa gaaatagcgg caatgtctgt ccgtccagtc gagcattggt gcaacactaa
    13021 accgaccact ccagtgaacg tcagtttttt caggcattgc gctggtttgg ttgatttttt
    13081 gcatttcaga attaccgtgc attttcaaat gtagagatta ttttatcgat atatcatggg
    13141 gttatgttat tcagcttcac tgttcaggag gctcaatagc ggggtactat accataacaa
    13201 caggaagcgc ctgtctcatt gcaaaagaaa attgagatcc tctcaaggca tgaagctctc
    13261 acgaagtgat ggaaataatc ttattagccg ttaactttgt taaggccaat gataaacaat
    13321 ccaggttcga cgataaataa aaaatcacac attaaactct ggtgatatat ctccctgcta
    13381 atagcattga tagagaaaaa agaacccaat aaatattggg tccttttata taatgccttc
    13441 catactatcg aagaacttca catattattt ctccgattta accccgaaca agtcataaat
    13501 taatttagaa gtgtctgtaa gtattttaat ctcttccttt gaggttgggt caaacgactt
    13561 cgcgaagcta attaattgtg gtgcagcatc tcgcattttg cttaaaatat caggatcgag
    13621 cgttccttca ttcaccagat gtgacatctt atccagatag ccatcaaaat tcatttctcc
    13681 cccatctgcc agacgcttca tctctcccag gtacttcttc atatcacgtt gatataattt
    13741 ttcaaactga gctttcaggt agctctcatt attttcatta atatacactg tttctgataa
    13801 atctgcgtag gcactagaat atgaaagtga aattaacagc gaagcaaata attttaagct
    13861 gttgtcatca tgttcctggc aggcattaac aaacgttaaa aaaccagaac ctattgcttg
    13921 aaaatgtata ttcgctaaag gttcattatc cttagattcc tcataaaata gctgaaccgt
    13981 ggagggaagg ccattcttag tttctacatc aaaagtacat tgttcaatgg gcattgcatc
    14041 tttattatcc ccaggattac atatactatt aatcgcgaaa tgaaaaatag cccggtcaac
    14101 aactgatgca ataaagtcac gatcgtttaa atcctcatca gtgcaatcgt cagtatactt
    14161 attgacccac attttgagat catttatttg ctcaattaaa aacacatttt tttacgtgca
    14221 tgccataaat tatgcgtgat tcgttgaata aataaatcta tttttgcaat aattatatcc
    14281 cgatgacgca gcgctgcata atcaatagat ttaccatata tattatttaa tttacctttg
    14341 attgaacctc tattatccaa tttatctttg gttgcatctt tattttcgaa ttgatttgaa
    14401 ttgagtgttt tattaaagta tccagttgca ccgggtatta tcttcaacat gattcatcta
    14461 ccttttttgt cgtaccgtgt cctccttata attctttttc catctggtca ctatcaaaaa
    14521 tcgctcataa tttctgaact acaggtatgc gtttaaaccc agttaaataa atagcgagtt
    14581 aacttgaagc aatttattta aaaatgaatt attaattatt atcgtgttga taatggtacg
    14641 acaaggcaac acaacacctt acctacaaaa cagaaaatca acattacggc aacaataagg
    14701 gttctcggtg tttttcctgg aacatggtaa agtaaggaca ttcttaaccc ccactttgag
    14761 gtgcccgatg gaaaagacca caacgcagga gttattagcg caggctgaaa aaatctgcgc
    14821 gcagcgtaat gtgcgcctga ccccacagcg cctggaagtg ttgcgcctga tgagtctgca
    14881 ggatggcgct atcagcgctt atgatctgct tgatttactg cgcgaagctg aaccgcaagc
    14941 caagccgcca acggtttatc gcgcgctgga ttttctgctt gagcaaggtt ttgtgcataa
    15001 ggtggaatcc accaacagtt atgtgctctg tcatctgttc gaccagccca cgcatacgtc
    15061 agccatgttt atttgcgatc gctgcggcgc ggtgaaagaa gagtgtgcag aaggcgtgga
    15121 agacattatg catacgctgg cggcaaaaat ggggtttgcc ctgcggcata atgtgattga
    15181 agcacatggg ctctgtgcgg catgcgtgga agtggaagcg tgtcgtcatc ctgaacagtg
    15241 ccagcatgat cactctgtgc aggtgaaaaa gaaaccgcgt taagagggcg tacatccttg
    15301 tacacgtcgg gcaggaggga ttaattacca gcgatattca ttgcgggttt cccaatccac
    15361 gacctctttt tctgcctgat ctttctgata accataacgt tcctggattt taccgaccag
    15421 ttgatcacgt ttaccttcaa tgatcgtcat atcatcatcg gtcagtttgc cccattgctc
    15481 tttcacttta cctttaaact gtttccagtt accgccggct tcatctttat tcataatcaa
    15541 gacctcatcg ttaggttgtg aatgagagta cgttcacttt tcttctgaac gtgagattaa
    15601 gtgtagtcag caatttggct taggataatt tattcagaat ttttaaccgt cacgttgcgg
    15661 caaaccaggt accgttgcgc cagtgacgcc gccagatagc cgccagagaa agcccgcgca
    15721 acgccagaaa gacggttaat gccagccaca aaccatgatt acccagccac ggcagcgtaa
    15781 ggagcgtcag cgcaaaacct gcggcggcca ccgccatact gttacgcatt tcggcggcac
    15841 gcgttgcgcc tataaacatg ccgtccagca gataacacca gacgccaacc aacggcaaaa
    15901 tcacctgcca gataagatag cggtcagcca gctgctgaat ctgggttaac gacgtcagca
    15961 acgcaatgat gtgttcccct gccagcaaat aaaccaccga aaacagtaac gctacaatcc
    16021 ccgactggcg gcacgctgcc cgccatacat ccagcaactg gctaccgtcg cgcgcaccgt
    16081 atgcctgacc ggagtgcgct tcaaccgcgt aggcaaaacc atcaagcgca taggcggtaa
    16141 aggtgagtag cgtcatcaga accgcgttaa cagcgataat gtcactcccc agtcgcgcac
    16201 caagtacggt gatcgcgccg aaacagagtt gcaacaacag cgaacgcagc atgatatcgc
    16261 ggttaagcgc cagcaagcga cggaagtttc ctcgccaggc agttttcagc atttcgccgg
    16321 aaattccgcg tagtttgagg attttacgca ccattagcag accaatcagc aatgttgcat
    16381 attccgcaat aaccgtcgcc agcgccgcgc cctgcacgtt catatgcagc cccatcacca
    16441 gccagacatc cagcacaatg ttgaggatat tgccgaccac taacaaaatt actggcgcac
    16501 gggcatattg cacgccgagt aaccaaccga gtaataccag attcgccagc gacgccggag
    16561 cgcttaacca gcggatttca agaaagcgcc gcgcctgttc caggaccgct tcactaccgc
    16621 caacaatatg cagcgccaga tcgataatcg gcgtacgcaa cagcgcaatt aacgccccgg
    16681 cccccaacgc caacagcaac ggttgcacca gcgcacgggc taatgcctga ggatttttgg
    16741 caccataagc ctgcgcagtc agcccggtgg tgctcatgcg taaaaacagc aacagcataa
    16801 agagaaagct ggtcgccgtt gcaccaaccg ccacgccgcc caaataaacc ggactatcaa
    16861 gatgaccaat taccgccgta tcgaccagtc ccagcaacgg aacggtgata ttggagaaaa
    16921 tcatgggtaa ggcgagatgc cagagtgctt tatcagatga agtgaggaat gccatgcaga
    16981 caagcctgat gaagagagat gaaaaacaaa ccgcgacgcc aggcggcatc gcggtctcag
    17041 agatatgtta cagccagtcg ccgttgcgaa taaccccaac cgccagccct tcaatggtga
    17101 agctctgctg acgaaggtca acgacaattg gtttaaactc gctattttct ggcaacagtt
    17161 cgactttatt gccctgtttt ttcaggcgct taacggtaac ttcgtcatca atacgtgcga
    17221 caacgacctg accgttacgt acatcctgag ttttatgcac tgccagcaag tcgccatcca
    17281 taatgccgat atctttcatc gacatcccgc tgacgcgcag caggaaatca gcattcggct
    17341 tgaacaagga aggatcgacc tgataatgac cttcaatatg ctgttgcgcc agaagcggtt
    17401 caccggcagc cacacgacct accagcggca acccttcttc ctcttcctgc aacagacgaa
    17461 tcccgcgtga tgcgccggaa acaatttcaa taacgccttt gcgtgccagc gccttcagat
    17521 gttcttcagc cgcgtttggg gaacggaacc ccaaacgctg cgcgatttcc gcacgcgtcg
    17581 gcggcatacc tgtctggctg atgtgatcac ggatgagatc aaacacctct tgttgcctgg
    17641 ccgttaacgc tttcattccg ccccctgggt gtatatacag ttatactgtg agtatataca
    17701 gcaaaaggcg attttggaac cataaactgc acaataaacc agagatttat cgaaatctgg
    17761 aagggttatc caaaatgcga ccataacagg atgcaccagg taatcacggc gacgataatg
    17821 gcaatcagca ccgcggcgga tcccatatct tttgcgcgtc cggaaagctc atggtattca
    17881 gagccaattc ggtcaaccac tgcttcgatg gcgctattga ggatttccac aatcatcacc
    17941 agcatcacgg agctgataag cagcacgcgg gtaattgcgt ccacatccag ccagcaggcg
    18001 atgaccaccg ccaacaatac cgctacgcct tcctgacgga atgccgcttc gttgatccat
    18061 gcagcgcgta aacctttcca ggaatagcca gcagctttga taattcgggt gaatccagtg
    18121 gtattattgg ccattgaaag aaccttttta cattatgagc gtcaatatca gtgtaccgtt
    18181 aaatccgatg attcgggttt gcgtaacgcc gcgcgaaaca tgagcggata ccacagaatt
    18241 tcccatgact ttctgctatc cttgccgcgc atttgcatta ttaaccagag gctttacatc
    18301 gtttatgtcc ggctggccac gaatttacta caaattactg aatttaccat taagcatcct
    18361 ggtaaaaagc aagtctattc cggcagatcc tgccccggaa ctggggctgg atacctctcg
    18421 tccaattatg tacgttttac cgtacaactc gaaagcagat ttgctgacgt tgcgcgccca
    18481 gtgtctggca catgatttgc ctgacccgtt agagccgctg gaaatcgacg gcacgctact
    18541 gccgcgctat gtgttcattc acggcgggcc gcgtgtattc acctattaca cgccgaaaga
    18601 agagtctatt aagcttttcc acgactatct cgatttgcac cgtagcaacc caaatctgga
    18661 tgtgcagatg gtgccagtat cggtgatgtt tggtcgcgcg ccggggcgtg aaaaaggcga
    18721 agtgaacccg ccgctgcgta tgcttaacgg cgtacagaaa tttttcgctg tactgtggct
    18781 cggtcgcgac agttttgtgc gtttctcgcc gtcagtgtcg ctgcgccgta tggcggatga
    18841 acacggcacg gataaaacta tcgctcagaa actggcgcgc gtggcgcgta tgcactttgc
    18901 ccgtcaacgt ctggctgccg taggcccacg tctgcctgct cgtcaggatc tgtttaataa
    18961 gctgctcgcc tcccgcgcca ttgccaaagc ggtagaagat gaagcgcgca gcaaaaaaat
    19021 ctcccatgaa aaagcgcagc agaacgcgat tgcgctgatg gaagagattg cggcgaattt
    19081 ctcttacgag atgatccgcc tgactgaccg cattctgggc ttcacctgga accgatttta
    19141 tcagggcatc aacgtccata acgccgagcg cgttcgccag ctggcccacg acggtcatga
    19201 gctggtgtat gtgccttgcc accgcagtca catggactac ctgctacttt cttacgtgct
    19261 gtaccaccag gggctggtgc cgccgcatat cgccgccggg atcaacctga acttctggcc
    19321 ggccgggccg attttccgcc gtctgggggc gttctttatt cgccgtactt ttaaaggcaa
    19381 taaactttat tccaccgttt tccgtgagta tctcggcgaa ctgttcagcc gtggttattc
    19441 cgtcgagtac ttcgtggaag gcggtcgttc ccgcacgggg cgtttgctgg atccgaaaac
    19501 cggtacgctg tcgatgacca ttcaggcgat gctgcgtggc ggcacgcgtc cgattacgct
    19561 gattccgatc tatatcggtt atgagcacgt catggaagtg ggtacttacg ccaaagaact
    19621 gcgcggcgcg acgaaagaga aagagagcct gccgcagatg ctgcgcggtt taagcaagct
    19681 gcgtaatctc ggtcagggtt acgtcaactt cggtgaacca atgccgttga tgacctacct
    19741 taaccagcac gtaccagact ggcgtgaatc tatcgatccc atcgaagcgg tgcgtccggc
    19801 ctggttaacg ccgacggtca ataatattgc tgccgatctg atggtacgca ttaacaacgc
    19861 aggcgcggca aacgccatga acctgtgctg tactgcgcta ctggcatcac gtcagcgctc
    19921 actcacccgc gagcagttaa ccgagcaact caactgctac ctggatctga tgcgcaacgt
    19981 accttactcc acggactcta ccgttccttc agccagcgcc agcgagctta tcgatcacgc
    20041 gctgcaaatg aacaagtttg aagtcgagaa agacactatc ggcgacatca tcattctgcc
    20101 acgcgagcaa gcggtgctga tgacctacta tcgcaacaac attgcgcata tgttggtgct
    20161 gccttcgctg atggcggcaa ttgtcaccca gcatcgccac atctcccgcg acgtattgat
    20221 ggagcacgtc aatgtgcttt acccaatgct gaaagcggag ctgttcctgc gctgggatcg
    20281 cgacgagttg ccggacgtta ttgatgcgct ggcaaatgag atgcaacgtc aggggctgat
    20341 taccctgcaa gatgatgagt tgcatatcaa cccggcgcat tctcgcaccc tacagctgct
    20401 ggccgcaggc gcgcgcgaaa cgctgcaacg ttatgccatc accttctggt tgttgagtgc
    20461 caacccgtcg atcaaccgcg gtacgctgga gaaagagagc cgcaccgttg cgcaacgtct
    20521 ctccgtgctg cacggcatca acgcgccgga gttcttcgac aaggcggtgt tcagttctct
    20581 ggtgctgacg ctgcgtgatg aagggtatat cagcgatagc ggcgatgccg aaccagcaga
    20641 aacgatgaag gtttatcagt tgctggcgga gttgattaca tcagacgtgc gtttgacgat
    20701 tgagagtgcg acgcagggcg aagggtaatc agagagaatt gccggatgcg gcgaaaacgc
    20761 cttatccggc ctaccatgac ctgcaaattc aataaattgc gattcaccgg gtaggcctga
    20821 gaagcgcagc gcatcaggca acccagaaga aagccggatg atcatccggc ttttttacat
    20881 catcagaaat gccagtaact cattgccagt cctaaaaaca gtaccagacc aacatagtta
    20941 ttattcataa atgctttaaa gcaggcttca cgctcgcggt tggcaatcag tttttgttga
    21001 taaacaaaca gcgcgccagc caccacaatt gaccagtaat atccccagcc taagccattt
    21061 aactcaccga tgatcgccat cagtgccagt acgccaatct gcaaaatacc aataatcaat
    21121 ttatcgtatt ggccgaacag gattgccgtg gatttaatgc caatcttcac atcatcatcg
    21181 cggtcaacca tcgcatactg cgtgtcgtaa gccaccgccc agagaatatt ggcgaggaac
    21241 attaaccagc aactcaatgg caccgactca ctcacagcgg caaaagccat tggaatcgac
    21301 cagccaaacg ccgcgcccag caccacttgc gggagatggg tataccgctt cataaacggg
    21361 tacacccacg ccaatgccag agcggcaatc gacaacagaa tggtcatcgt attcagcgtc
    21421 agaaccagta aaaacgaaat cagtaccagc actacaaaca gcgcgcgcgc ctctttctct
    21481 gttaccgcgc cgctgggaag tggtcggttc gccgtgcgct taacatgacc atcaaacttg
    21541 cgatcagcat aatcattcac cacgcaacca gcagcgcgca tcagccagac acccgcgaca
    21601 aacaccgcca ggatccagag ctgaggaacg cccggtgtcg ccacccacaa cgcccataat
    21661 gttggccaga gcagcagtaa cgcgccaatt ggcttatccg tacgcattaa gcgatgaaac
    21721 gccagcagct tattctgcgt cagactccac tccatatttt tttcctctta gtacaacggt
    21781 gacgccggta aaaacagttc tgttagcaac agcggtttac cgcttaatcg caggcgggaa
    21841 cgtcgccccc acagtccggc atcacggcct atctcgataa aatcccgggt tagtgtcgat
    21901 gatgtgaaca gatagcgtcc taacggcgtt ttacccaatt tttgtaacgc tagttccggc
    21961 ccgcttaatg ttgatacagg aacgacggta cgaccggcaa gccacggttc accatcggca
    22021 cataacaaaa tttcacgtaa ccagtaacga gactctttcg gcagtagcgg cagttcttcg
    22081 gggatttcat tctgctcgac aaacccctcg cggatcatcg tcacactcac cgtttttccc
    22141 tgctgttcaa aacgttttgt catggaatcc tccagcaaca gccagtcgaa cagttgcgga
    22201 tccagggcag ggatctcttt aaaatagcgc agcgcacgca gttgcgttaa cgcggggtgt
    22261 gacatgccga actctccgtt acataacgta aaggcattgt atcgcagaaa agtgtttacg
    22321 ggagatgtaa agcaaattaa gtgatctaat gcgcaacaaa aacgcaacaa tcgcatgaag
    22381 tggtaaaacg ctgttgaacc ttacgctttg ccgatgcccg tgctggcgag ggcaaccata
    22441 ggggcatcac cgcccatgat gctaggaacg gtgatgtttt cctttactta aataccttta
    22501 ttctttatac gagtagctcg ccaactcgca gtaaaaattg ggaaaagcgc atatttacca
    22561 tgagatttgg agctgaaaag tgtttcctga gtaagattaa taggcaacat cttatgtctg
    22621 gatttataga tacacgtgga attccaattg gtatcttttg taggattcaa catatcatgt
    22681 aagcgctggg agcttaccac cataacaggc ccatcatcga cagaggagaa caggataaat
    22741 atattatcgt ttcgtgttaa tttattagtg ttaaaggttc ttaacttttc ttcagtttct
    22801 ataaatatag gtgaaacatc atcactacga atccactctt tataaagata ccaggcattt
    22861 gctttgatca tatcatccgt tgatcgtaag gcctgatcga taagttggat aaaaaaattg
    22921 ttatttttat caaccagccc tttattcata ttaaatctgt taataaaatg gatcatctgt
    22981 tgggtatcga aattcacctt actgaatcga tgttgacgca taaaattatc gaacttgtca
    23041 tcttccccag aatgtatata actacgtata attggatctt ttatataagg acaatggcta
    23101 aatgttttct tcatattctc aataaagttt ttattaacaa tattgagaat ttttaattgc
    23161 atcaccagtt ttactgccag aacggatttc aatatcattg cattatctgg caatgtatca
    23221 atcattttta taatgttatt gatataacca tcgagatctt cattcccaaa tatatctgaa
    23281 taatcaatag ttaattccaa ttgttctgta attcaatctt aaaaattccc tgtcgacatt
    23341 cgcacatgat caaacataac agctgtcaaa ttcgatttaa taaatgatat ttctttggca
    23401 gacagaaact ctgctctggt gaagtgcgaa gcgtaaagat aacaattatc gaagtttgaa
    23461 tttctaatct gacattcgcg aaaattacta tgaccaagtt gactctgcga gaagtcaact
    23521 gtatcaagaa tattttggct aaaatcaaca cattcgccac tcgttttgag aaaaatcaca
    23581 ttgtgtaaac gtcgacattt aaatatgaca ttatgaactg ttgccttagt aaaattgcaa
    23641 ttctcaattt ctttacattc gaaagttaca tgttgtagat ttgatgataa aaaagacacc
    23701 ttagaaataa cgtcaccttt aaaatgacaa tttgttaaat caaaatcaga caggtcgata
    23761 tacccctgcg catctttatc catcagaaaa ttacaagcgc attctatttt ttcatttctg
    23821 tagaatttta cacttatagt atcattacta attgagactt cacgtaacat atttattgca
    23881 tgattttttt cgttccgttg ttttatattt aaaaatatat cactgatata aataaagtta
    23941 tctctgttta aacctgtcac cccattgaaa ttacgtagta aatcaagatc tactatatta
    24001 tttccttgca ccacatcatg tttcatcaaa gagttattgc cgccagaatt tgtgatatga
    24061 cctatcataa aacctctgta atacagaaat aaaataacct ggaaaagtag cctgagcgtc
    24121 cagacactac cggcgaaagg aaaagaatct ctgataatgc attgaaataa tggacattct
    24181 tagtgaagaa gaagtgaagt actataaaaa atcgctcata tcccaggaag gaagcataaa
    24241 aaaaggtgcg ccaggagacg caccagttgt tgcaaaatca gcactgtggg gcgtaattac
    24301 cccttgcctt ttacactgct gataaaggtg gaacgggcag atgtcgatcc caggcgttca
    24361 gcttcatcaa gcagttttaa cgccttatca acatcacctt tcgcgacagc gtttttgatc
    24421 gcggtattaa aataactttc cgtgtcgttg agcatcggct cgcttttctt caccggcacc
    24481 ggagcaggtg cagccacagc tggtgccgcc gtgttgccta ccgtaaccgg agctggagcg
    24541 gaagaaccaa atagtggtcc taccaacacg ctggagctgg agttcgtttt gactttcagt
    24601 ttcagtaagc catcgctggt atgacgagca accggatcgg gaatatccgg gatcgagtta
    24661 ccgacgcctt tggcataggc tttagccggg tcgagcagtt gggtcgtctg ctggagatct
    24721 ttttccatgg taaagaccag aacataaagt ttttgctgcc ccaacgccgg tgtcaggcgc
    24781 ataacgcctt ccagccgatc tgcactcatc acgcctagtt cctggtaggt gaaataactg
    24841 ctggggaaga aggctgatgg ggtcatgttc tgatcaataa tcagcacgtt cggcgcaaaa
    24901 acgctggttt gtttgttcac ttcgctggtc agcgtcaggg tcagttcgcc aatgtttgcc
    24961 gggacgctgt acgcagcaac cggaccactg atgccgggaa cgttcagttg ttggccgccg
    25021 gtcgccagtt gggtggtctg gattttagat tgatcgaccg gtgtccaggt gagttgttgc
    25081 agcgcggcag atggaatggc tggcgcgtcg ctggtgtttt gcggtacgta gttaacatcg
    25141 gcaaggctaa ttccaggcgc gcttgccagt aaccctgctg ataaacagag ggcgatgaga
    25201 cttttattca ttttcattgt tttcacctca aaatctggag ctcagcggta gccaggcaat
    25261 agcgcgctaa acccgataat cagaggggct ttcgcccctt tcagataatg acaacctgtt
    25321 tttatgccgg atgcggcgta aacgccttat ccggcccagg ttttgctatt accaccagat
    25381 ttccatctgg gcaccgaagg tccactcgtc gctgtcgcca cgaccgaagc tgccgccgtt
    25441 gaaatcagca ggaacggctt tgccgtagtt cgggttaacc ttgctatcgc cgttgtagtc
    25501 gtaaccccat ttctcatccc acttggcgta ggttgcgaag acacgaatag ccgggcgtga
    25561 ccagatgctg tcgccagcct gccattgttg tgcgagggta attttgtact gattgttctt
    25621 gtcgccggtg cgctgggatt cgacgttgtc gtagccgatt tccatcacgg tgctcatgat
    25681 tggcgtccac ttgtacatcg ggcgaatacc aacggtccac cacttggtgc cgttgtcgtt
    25741 atcccagttg atatcctggt acatacccac gtacatcatg tcccagttgt cgcccatgga
    25801 gatcgcaccg tggtcgagga tacgcagcat gtgaccgttg ttgttgatat tgtaggcaaa
    25861 tttttcgtta tcaaacgcga cgccagaacc ctgcgacaga cctttaccct gcgaggtcat
    25921 cgagtcagta gcgtactgaa caacaaactt gttaaagccc ttcaggacac tctgagtatg
    25981 ttcagcagtg aataaccagc cgtctttcga tgcgccatca accagacgat agttatcacg
    26041 caggttggca cgaccgtagt cgacacccag ttctaatgtg ccgcccgggt tgatttccat
    26101 ctgcgctaaa cgcacatcga aaacgtcgtt cgcggtttcg ttggtatagt cataaatatt
    26161 gttgctggca aaagaggaag aaccaccagc ttcagaggag cgggttgctg ccagagagag
    26221 tttaccgaag ccaacatcga tgttttccag accggcacca ggaccagaaa tatcccagta
    26281 gtagaagtcg atcatatgaa cgtcatgacg ttggtagaag cgcttacctg cccagatggt
    26341 ggaacctggc agccattcga tcaggttttt tccctgcacg tttgcttcac ggaaggccgg
    26401 atcggtagct tcccagtcat tctgttgcgc gacggaatag gccacgttag tgtcgaaata
    26461 gaagctctta tcgccctctt tccacacttc ctgacccaat tttaattcag cataagtttc
    26521 acattcgttg ccaagacggt atttactttg agcaccggta gtctggaaac actgttgttc
    26581 accgccgcta cctgtccagc caataccgga acgtgcatag ccgtggaaat caacagccat
    26641 tgcctgagca gacattacgc ccgctgcgac ggcaaccgcc agaggaagtt tgcgcagagt
    26701 aatcatcatt ctatctcctg agtcattgct tttctttttt cacatcacct gtgacaggct
    26761 ttgtgtgttt tgtggggtgc ttaaacaccc ggctccttat gcagtcgacg acatgcagtg
    26821 ccatcctcac ggaacagatg gcaacgctct ggcggcaggc cgatagcgaa tgtggcacct
    26881 tcttctacca acaccacgtc gttctggcgg tacaccaggt tttgacgaat ggaagggatc
    26941 tggatataga tttgggtttc gttgccgagt tgctcgacga cctgaacttc accctcaagg
    27001 atgacgtcag cgatatcact cggcagtaga tgttccgggc gaatacccag cgacatattg
    27061 gctccaacct ggacatcacg gctttcaaca ggcagccaga cttgttgacg atttggcatc
    27121 ggcagctcca cctgcacttg atcgattgcg gtggcggtca cttttaccgg caggaagttc
    27181 atctttggcg aaccgataaa tccggcgaca aaacggtctg ccggatagtg gtacagttcc
    27241 agcggtttcc caacctgtgc cacgcgaccg gcgtccagca ccacgatttt gtcggccagc
    27301 gtcatcgctt cgacctgatc gtgggtgacg taaatcattg tgcggcccag gcgtttatgc
    27361 agacgggaga tttcgatacg catttgcaca cgcagtgcag catcgaggtt ggagagcggt
    27421 tcatcgagca aaaatacgct tggctcggcc accagcgtac ggccaatcgc cacgcgctga
    27481 cgctgaccac cggagagcgc tttcggtttg cgatccagca aatgcgccag ttgcagcact
    27541 tccgccacct ggttgacgcg ttggttaatc acctcttttt ttgcgccagc cagtttcagg
    27601 ccaaatgaca tgttttctgc tactgacagg tggggataga gcgcgtaaga ctgaaacacc
    27661 ataccaacgc cgcgttctgc tggcggagtg tcattcatcc gtttctcacc gatgaacagg
    27721 tcgccgctgg tgatcgtctc aagcccggca atcatgcgca gtaaagtcga tttaccgcag
    27781 ccagacggtc cgacaaacac cacgaattca ccttcatgga tatcgagatt gatatctttc
    27841 gataccacga cctcgcccca ggctttcgtt acattttgca gctgtacgct cgccatgccc
    27901 ttctcccttt gtaacaacct gtcatcgaca gcaacattca tgatgggctg actatgcgtc
    27961 atcaggagat ggcttaaatc ctccaccccc tggctttttt atgggggagg aggcgggagg
    28021 atgagaacgc ggcttctgtg aactaaaccg aggtcatgta aggaatttcg tgatgttgct
    28081 tgcaaaaatc gtggcgattt tatgtgcgca tccccacatt accgccaatt ctgtaacaga
    28141 gatcacacaa agcaacggtg gggcgtaggg gcaaggagga tggaaagagg ttgccgtata
    28201 aagaaactag agtccgttta ggtgttttca cgagcacttc accaacaagg accatagatt
    28261 atgaaaataa aaacaggtgc acgcatcctc gcattatccg cattaacgac gatgatgttt
    28321 tccgcctcgg ctctcgccaa aatcgaagaa ggtaaactgg taatctggat taacggcgat
    28381 aaaggctata acggtctcgc tgaagtcggt aagaaattcg agaaagatac cggaattaaa
    28441 gtcaccgttg agcatccgga taaactggaa gagaaattcc ctcaggttgc ggcaactggc
    28501 gatggccctg acattatctt ctgggcgcac gaccgctttg gtggctacgc tcaatctggc
    28561 ctgttggctg aaatcacccc ggacaaagcg ttccaggaca agctgtatcc gtttacatgg
    28621 gatgccgtac gttacaacgg caagctgatt gcttacccaa tcgctgttga agcgttatcg
    28681 ctgatttata acaaagacct gctgccgaac ccgccaaaaa cctgggaaga gatcccggcg
    28741 ctggataaag aactgaaagc gaaaggtaag agcgcgctga tgttcaacct gcaagaaccg
    28801 tacttcacct ggccgctgat tgctgctgac gggggttatg cgttcaagta tgaaaacggc
    28861 aagtacgaca ttaaagacgt gggcgtggat aacgctggcg cgaaagcggg tctgaccttc
    28921 ctggttgacc tgattaaaaa caaacacatg aatgcagaca ccgattactc catcgcagaa
    28981 gctgccttta ataaaggcga aacagcgatg accatcaacg gcccgtgggc atggtccaac
    29041 atcgacacca gcaaagtgaa ttatggtgta acggtactgc cgaccttcaa gggtcaacca
    29101 tctaaaccgt tcgttggcgt gctgagcacc ggtattaacg ccgccagtcc gaacaaagag
    29161 ctggcgaaag agttcctcga aaactatctg ctgactgatg aaggtctgga agcggttaat
    29221 aaagacaaac cgctgggtgc cgtagcgctg aagtcttacg aggaagagtt ggcgaaagat
    29281 ccacgtattg ccgccaccat ggaaaacgcc cagaaaggtg aaatcatgcc gaacatcccg
    29341 cagatgtccg ctttctggta tgccgtacgt actgcggtga tcaacgccgc cagcggtcgt
    29401 cagactgtcg atgaagccct gaaagacgcg cagactcgta tcaccaagta atgctgtgaa
    29461 atgccggatg cggcgtgaac gccttgtccg gcctacaaaa ctgaaacgca tgtaggcctg
    29521 ataagacgca tcagtgtcgc atcaggcagt tgttgtcgga taaggcgtga aagccttatc
    29581 cgtcctggaa tgaggaagaa ccccatggat gtcattaaaa agaaacattg gtggcaaagc
    29641 gacgcgctga aatggtcagt gctaggtctg ctcggcctgc tggtgggtta ccttgttgtt
    29701 ttaatgtacg cacaagggga atacctgttc gccattacca cgctgatatt gagttcagcg
    29761 gggctgtata ttttcgccaa tcgtaaagcc tacgcctggc gctatgttta cccgggaatg
    29821 gctggaatgg gattattcgt cctcttccct ctggtctgca ccatcgccat tgccttcacc
    29881 aactacagca gcactaacca gctgactttt gaacgtgcgc aggaagtgtt gttagatcgc
    29941 tcctggcaag caggcaaaac ctataacttt ggtctttacc cggcgggcga tgagtggcaa
    30001 ctggcgctca gcgacggcga aaccggcaaa aattacctct ccgacgcgtt taaatttggc
    30061 ggcgagcaaa aactgcaact gaaagaaacg accgcccagc ccgaaggcga acgcgcgaat
    30121 ctgcgcgtga ttacccagaa tcgtcaggcg ctgagtgaca ttaccaccat tctgccggat
    30181 ggcaacaaag tgatgatgag ctccctgcgc cagttttctg gcacgcagcc gctctacaca
    30241 ctcgacggtg acggcacgtt gacgaataat cagagtggcg tgaaatatcg tccgaataac
    30301 caaattggct tttaccagtc cattaccgcc gacggcaact ggggggatga aaagctaagc
    30361 cccggttaca ccgtgaccac cggctggaaa aactttaccc gcgtctttac cgacgaaggc
    30421 attcagaaac cgttcctcgc cattttcgtc tggaccgtgg tgttctcgct gatcactgtc
    30481 tttttaacgg tggcggtcgg catggttctg gcatgtctgg tgcagtggga agcgttgcgc
    30541 ggcaaagcgg tctatcgcgt cctgctgatt ctgccctacg cggtgccatc gttcatttca
    30601 atcttgattt tcaaaggatt gtttaaccag agcttcggtg aaatcaacat gatgttgagc
    30661 gcgctgtttg gcgtgaagcc cgcctggttc agcgatccga ccaccgctcg cacgatgctg
    30721 attatcgtca atacctagct gggttatccg tacatgatga tcctctgcat gggcttgctg
    30781 aaagcgattc cggacgattt gtatgaagcc tcagcaatgg atggcgcagg tccgttccag
    30841 aacttcttta agattacgct gccgctgctg attaaaccgc tgacgccgct gatgatcgcc
    30901 agcttcgcct ttaactttaa caacttcgtg ctgattcagc tgttaaccaa cggcggcccg
    30961 gatcgtcttg gcacgaccac gccagccggt tataccgacc tgcttgttaa ctacacctac
    31021 cgtatcgctt ttgaaggcgg cgggggtcag gacttcggtc tggcggcagc aattgccacg
    31081 ctgatcttcc tgctggtggg tgcgctggcg atagtgaacc tgaaagccac gcgaatgaag
    31141 tttgattaag ggagataaca aaaatggcaa tggtccaacc gaaatcgcaa aaagcacgtt
    31201 tgtttattac tcacctgcta ctgctacttt ttatcgcggc gattatgttc ccgctgctga
    31261 tggtcgtcgc tatctcgctg cgtcagggaa acttcgcgac cggtagcctg atcccggagc
    31321 aaatctcctg ggatcactgg aaactggcgt taggttttag cgttgaacag gctgatggtc
    31381 gcattacgcc accgccattc ccggtactgc tgtggctgtg gaactcggta aaggtcgctg
    31441 ggatttccgc gattggcatt gtggcgctct ccaccacctg cgcctacgct ttcgcccgta
    31501 tgcgctttcc aggcaaagcg acgctgctga aaggaatgct gattttccag atgttcccgg
    31561 cagtactttc actggtcgcg ttgtatgcgt tgtttgatcg tctgggtgag tacattccat
    31621 tcattggcct gaatactcac ggcggcgtaa ttttcgcgta tctgggtggg attgcgctgc
    31681 atgtctggac catcaaaggc tatttcgaaa ccatcgacag ttcgctggaa gaagcggctg
    31741 cgctggatgg tgcgacaccc tggcaggcct tccgccttgt cctgttgccg ctgtcagtac
    31801 cgattctggc ggtggtattc atcctgtcgt ttatcgctgc cattactgaa gttccggtcg
    31861 cgtcgctgtt actgcgtgac gtaaacagct acaccctggc cgtggggatg cagcaatacc
    31921 tcaacccgca aaactacctg tggggtgact ttgccgccgc tgccgtgatg tctgcattac
    31981 cgatcaccat cgtcttcttg ctggctcaac gctggctggt caacggcctg acggcaggtg
    32041 gtgtgaaagg ttaaagatgt tgttctgcca atgttatgcc gctgcaccct caacttacgt
    32101 tatcccaact tgtgactatt attcggcgct ccacggagcg cctttttttc tttcgtctgc
    32161 aatctgaatc gttcgccggt taatattccc atcatagaac ttattatttt tacgttattt
    32221 gttttcccac ttacgataat tctctttcgt gctctgagtc acggcaatag tattgttttc
    32281 atcaattttt gataattatc acaattaaga tcacagaaaa gacattacg
//
LOCUS       DADMEQ010000036        32129 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.036, whole genome shotgun sequence.
ACCESSION   DADMEQ010000036 DADMEQ010000000
VERSION     DADMEQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.036"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..537
                     /locus_tag="JNZ05_001694"
     CDS             <1..537
                     /locus_tag="JNZ05_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564961.1"
                     /translation="VLLTRQGQLVETFISTVEQWRDQKQIITIVCKDGISITDSIITQ
                     FKASKEHTIANGLRIQMTFQGVEFRSIVGQTDISAATGKTATTNDGGATSKKNTGNTT
                     TSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSVKNGESTFTASANERASKVLK
                     SGNALRKYSVNPNKKGTY"
     gene            complement(570..2090)
                     /locus_tag="JNZ05_001695"
     CDS             complement(570..2090)
                     /locus_tag="JNZ05_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY8564962.1"
                     /translation="MRTVITYLRFSSATQGAEGADSTRRQNELFKQWLRKNSDAQVVA
                     SFNDEGLSGYKGKHLSGQFGDMLSRIESGEFPEGTILLVEAIDRIGRLEHLETEALMN
                     RIIAHGIEIHTLQDGLIYTRDALSDDLGISIIQRVKSYIAHQESKQKSFRVSQKWEQR
                     AKLALAGEQRLTRNVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQANNI
                     SSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEAAIDAATFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDTMKRKASLTDKELPQLKDIDLTTKAGRVECQLI
                     LSKHLKGLTLGKGSVTITLQNDTEITIPTDPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            2181..2912
                     /gene="tonB"
                     /locus_tag="JNZ05_001696"
     CDS             2181..2912
                     /gene="tonB"
                     /locus_tag="JNZ05_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HAY8564963.1"
                     /translation="MPLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     KPKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRAL
                     SRNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWR
                     YEPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(2952..3350)
                     /gene="yciA"
                     /locus_tag="JNZ05_001697"
     CDS             complement(2952..3350)
                     /gene="yciA"
                     /locus_tag="JNZ05_001697"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HAY8564964.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3455..3994)
                     /locus_tag="JNZ05_001698"
     CDS             complement(3455..3994)
                     /locus_tag="JNZ05_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HAY8564965.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAVALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(4024..4767)
                     /locus_tag="JNZ05_001699"
     CDS             complement(4024..4767)
                     /locus_tag="JNZ05_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HAY8564966.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVCALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(4795..4884)
                     /locus_tag="JNZ05_001700"
                     /pseudo
     CDS             complement(4795..4884)
                     /locus_tag="JNZ05_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            5124..5762
                     /gene="ompW"
                     /locus_tag="JNZ05_001701"
     CDS             5124..5762
                     /gene="ompW"
                     /locus_tag="JNZ05_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HAY8564967.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGS
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(5822..6328)
                     /locus_tag="JNZ05_001702"
     CDS             complement(5822..6328)
                     /locus_tag="JNZ05_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY8564968.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(6374..6874)
                     /locus_tag="JNZ05_001703"
     CDS             complement(6374..6874)
                     /locus_tag="JNZ05_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY8564969.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLPRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(6960..7139)
                     /locus_tag="JNZ05_001704"
     CDS             complement(6960..7139)
                     /locus_tag="JNZ05_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HAY8564970.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSDKS"
     gene            complement(7520..8326)
                     /gene="trpA"
                     /locus_tag="JNZ05_001705"
     CDS             complement(7520..8326)
                     /gene="trpA"
                     /locus_tag="JNZ05_001705"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HAY8564971.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(8326..9519)
                     /gene="trpB"
                     /locus_tag="JNZ05_001706"
     CDS             complement(8326..9519)
                     /gene="trpB"
                     /locus_tag="JNZ05_001706"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HAY8564972.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(9531..10889)
                     /gene="trpCF"
                     /locus_tag="JNZ05_001707"
     CDS             complement(9531..10889)
                     /gene="trpCF"
                     /locus_tag="JNZ05_001707"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HAY8564973.1"
                     /translation="MQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLLI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            complement(10893..12488)
                     /gene="trpD"
                     /locus_tag="JNZ05_001708"
     CDS             complement(10893..12488)
                     /gene="trpD"
                     /locus_tag="JNZ05_001708"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HAY8564974.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(12488..14050)
                     /gene="trpE"
                     /locus_tag="JNZ05_001709"
     CDS             complement(12488..14050)
                     /gene="trpE"
                     /locus_tag="JNZ05_001709"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HAY8564975.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALHITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            14323..15204
                     /gene="yciV"
                     /locus_tag="JNZ05_001710"
     CDS             14323..15204
                     /gene="yciV"
                     /locus_tag="JNZ05_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HAY8564976.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            15201..15821
                     /locus_tag="JNZ05_001711"
     CDS             15201..15821
                     /locus_tag="JNZ05_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAY8564977.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKS
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            15922..16794
                     /gene="rluB"
                     /locus_tag="JNZ05_001712"
     CDS             15922..16794
                     /gene="rluB"
                     /locus_tag="JNZ05_001712"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HAY8564978.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(16834..17424)
                     /gene="cobO"
                     /locus_tag="JNZ05_001713"
     CDS             complement(16834..17424)
                     /gene="cobO"
                     /locus_tag="JNZ05_001713"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HAY8564979.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(17421..18179)
                     /locus_tag="JNZ05_001714"
     CDS             complement(17421..18179)
                     /locus_tag="JNZ05_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HAY8564980.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            18399..19448
                     /gene="sohB"
                     /locus_tag="JNZ05_001715"
     CDS             18399..19448
                     /gene="sohB"
                     /locus_tag="JNZ05_001715"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HAY8564981.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(19484..19735)
                     /locus_tag="JNZ05_001716"
     CDS             complement(19484..19735)
                     /locus_tag="JNZ05_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HAY8564982.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            20115..22712
                     /gene="topA"
                     /locus_tag="JNZ05_001717"
     CDS             20115..22712
                     /gene="topA"
                     /locus_tag="JNZ05_001717"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAY8564983.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            22922..23896
                     /gene="cysB"
                     /locus_tag="JNZ05_001718"
     CDS             22922..23896
                     /gene="cysB"
                     /locus_tag="JNZ05_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HAY8564984.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            24191..24355
                     /locus_tag="JNZ05_001719"
     CDS             24191..24355
                     /locus_tag="JNZ05_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HAY8564985.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            24358..24525
                     /locus_tag="JNZ05_001720"
     CDS             24358..24525
                     /locus_tag="JNZ05_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564986.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDRQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            24639..24734
                     /locus_tag="JNZ05_001721"
     CDS             24639..24734
                     /locus_tag="JNZ05_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8564987.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            24898..27573
                     /gene="acnA"
                     /locus_tag="JNZ05_001722"
     CDS             24898..27573
                     /gene="acnA"
                     /locus_tag="JNZ05_001722"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HAY8564988.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTQYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(27637..28227)
                     /gene="ribA"
                     /locus_tag="JNZ05_001723"
     CDS             complement(27637..28227)
                     /gene="ribA"
                     /locus_tag="JNZ05_001723"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HAY8564989.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            28397..29161
                     /gene="pgpB"
                     /locus_tag="JNZ05_001724"
     CDS             28397..29161
                     /gene="pgpB"
                     /locus_tag="JNZ05_001724"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HAY8564990.1"
                     /translation="MRSIARRTAVGAALLFVMPVAVWISGWRWQPGEQSWRLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFSILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            29310..29618
                     /gene="lapA"
                     /locus_tag="JNZ05_001725"
     CDS             29310..29618
                     /gene="lapA"
                     /locus_tag="JNZ05_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HAY8564991.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            29625..30794
                     /gene="lapB"
                     /locus_tag="JNZ05_001726"
     CDS             29625..30794
                     /gene="lapB"
                     /locus_tag="JNZ05_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HAY8564992.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            30987..31724
                     /gene="pyrF"
                     /locus_tag="JNZ05_001727"
     CDS             30987..31724
                     /gene="pyrF"
                     /locus_tag="JNZ05_001727"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAY8564993.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            31724..32050
                     /gene="yciH"
                     /locus_tag="JNZ05_001728"
     CDS             31724..32050
                     /gene="yciH"
                     /locus_tag="JNZ05_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HAY8564994.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
BASE COUNT         8079 a         7788 c         8238 g         8024 t
ORIGIN      
        1 gttctattaa ccagacaggg gcaacttgta gagaccttta tttctacggt tgaacaatgg
       61 cgagaccaga agcaaatcat cactattgtt tgtaaagatg gaatatctat cactgatagt
      121 atcattactc agtttaaagc gagtaaagag cataccattg ctaatggtct tcgtattcag
      181 atgaccttcc aaggtgtaga atttcgttca atcgtagggc aaactgatat ttcagcagct
      241 actggcaaga ccgctaccac gaatgatggt ggtgctacca gtaaaaagaa cactggcaac
      301 actacaacga gtcttggaag ccctatgctg acctgtaaag agctttttag ctattcggca
      361 agtgagttat cagatgaagc gctaaaagcc cgtgtaacgt gctctaagag cgttagcgtt
      421 aaaaatggtg agagcacctt cacagcctca gctaatgagc gagcttcaaa agtgcttaag
      481 tcgggtaatg ctttacggaa gtactctgtg aatccgaata agaagggcac ttattgagac
      541 ataaaaaggg gcttcatagc ccctgaaaat tacagttgat aagcatcaat atccagtagc
      601 tctttatcag caatttcaaa gattggtgac ccatcattta gcggtagcgg gtctgtaggt
      661 atggtgattt cagtgtcgtt ctgtagcgtt atagttactg aacccttacc caatgtaagc
      721 ccttttagat gtttggacaa aattaattga cactcgacac gacccgcttt agttgtcagg
      781 tcaatatctt taagctgggg tagttcctta tcagtgagtg aagccttacg cttcatagta
      841 tcaatcgtgg tctgtagttc tgtgcgcttc tggttaagtt ccttaagctg aatttttagt
      901 tcatcaatat catccatctc aagaagtagg gcggtagctt tcttgattct ggctgtgaca
      961 atttcaacct catgctgtaa cgctgcaatc ttcctctctg attctccatc attccccacg
     1021 tcaatcatcc ccagaaaatt atcaatcatc catcggtcaa acggtttacg cttcaacggt
     1081 ggaacatcac agcgaccgtc taagtggtta ttgcacctgt agaccccagc atacttattc
     1141 tttgttccgg taggatggac actagcccca cactgacacc taaacagacc tttgaagatg
     1201 ttaatcgtta gtgggttgtc acttgcaggt gtacggcctt tacgattctt atcaagaatc
     1261 tcttgagctt tattgaacgt tgcagcatct atagcggctt cgtagtagtt cggtatagcg
     1321 gggcggtcat tgcgctgtga tgctggtagt gtacctatcg ttgtctctga ccttaagata
     1381 gtacgcacag aatgaacgct aaatccatta gcatctttac gtcgtgagaa tgaactaatg
     1441 ttattagcct gtaggtgacg tgcaatgtta tgcagacttt caccatctag cagcagcttg
     1501 aaaatcaagc gtacagtttc agcatgttca ttgagtttaa aagtctcagg gtcaatccat
     1561 ccgggaacat tccttgttaa acgttgttca ccagcaaggg caagctttgc acgttgttcc
     1621 catttctgac taacacggaa agacttctgt ttactctcct gatgcgctat gtagctttta
     1681 acccgctgaa tgattgagat tcctaaatca tcggatagag catcccttgt gtagattagc
     1741 ccatcctgta gagtgtgaat ctcgatacca tgagcaataa tgcgattcat caaggcttct
     1801 gtttcaagat gttcaaggcg gcctatgcgg tcgatagctt cgactagtaa aattgtccct
     1861 tctggaaact cccctgattc aatgcgggag agcatatccc caaattgccc tgataagtgt
     1921 ttacctttgt aaccggatag accttcatca ttgaaagatg ccactacctg agcatcgcta
     1981 ttcttcctta accactgttt gaatagctca ttttgtcgtc tggttgaatc agcaccttca
     2041 gcaccttgtg tggctgatga gaatctcaag tatgttatta cggttctcac ggtgataaac
     2101 tcccatgcaa ttgattatca tttaaagatg aacttataaa cgttatggct aatgctacag
     2161 tgccgaatag ctacaataca atgccccttg atttacctcg ccgcttcccc tggccgacgt
     2221 tactttcggt ctgcattcat ggtgctgttg tggcgggtct gctctatacc tcggtacatc
     2281 aggttattga actacctgcg cctgcgcaac caatttctgt cacgatggtt gcgcctgctg
     2341 atctcgaacc gccacaagcc gttcagccgc caccggagcc ggtggtagag ccagaaccgg
     2401 aacctgagcc gatccccgaa ccgccaaaag aagcaccggt ggtcattgaa aagccgaagc
     2461 cgaaacctaa gccaaaaccg aagccaaaac cgaagccggt gaaaaaggta caggagcagc
     2521 caaaacgcga tgtcaaaccc gtagagtcgc gtccggcatc accgtttgaa aatacggcac
     2581 ccgcgcgccc cacatcaagt acggcaacgg ctgcaaccag caagccggtt accagtgtgg
     2641 cttcaggacc acgcgcatta agccgtaatc agccgcagta tccggcacga gcacaggcat
     2701 tgcgcattga agggcaggtt aaagttaaat ttgacgtcac gccggatggt cgcgtggata
     2761 acgtacaaat cctctcagcc aagcctgcga acatgtttga gcgtgaggtg aaaaatgcga
     2821 tgcgcagatg gcgttatgag ccgggtaagc caggcagtgg gattgtggtg aatatcctgt
     2881 ttaaaattaa cggcaccacc gaaattcagt aagcagaaag tcaaaagcct ccgaccggag
     2941 gcttttgact attactcaac aggtaaggcg cgaggttttc cttcaggatc aaccgcgaca
     3001 tacttaaata atgcttctgt cgctttatag cgttgcccga ttggttcaga cgctactttt
     3061 ttcacccaca cttcaatatt aatgctgacc gatgtcgtcc ctttctggac acagcgtgca
     3121 tagcagcaca ccacatcgcc gaccgcaacc ggccgtaaga aagtcattcc ttcaacccgc
     3181 acagtcacta cgcgaccgtg ggcaatttct tttgccagaa tagcgccgcc aatatccatt
     3241 tgtgacatta accaaccacc aaagatgtca ccattggcat tggtatcggc gggcatggct
     3301 aaagtacgta aaacaagatc gccctgaggg acgttatgtg ttgtagacat ggtaaaaccg
     3361 acttaaaagg aaaatcacag gcgcgatgct actatgattt tcctgaggag aacagaggag
     3421 atacagcgcc agccccgaag gactggcagt ctggttagga tttatcttcc tgcggcatgt
     3481 ggcggtagat atagataccg cttaacaatg tgaagattaa ggtaagggcg gtcaggccaa
     3541 agactttaaa gttgacccaa atattttgcg gcagccagaa tgcgatgtag atgtttgcca
     3601 gaccgcaaag gataaagaaa acagcccagg ccagatttag cttcgaccat accggttgcg
     3661 gcagcgtgag ttctttaccc agcatccgct gaattagcgg ctttttcatc acccattggc
     3721 tgactaacag ggctaccgca aacagagcat aaatgactgt aaccttccat ttaataaact
     3781 catcattgtg gaagaacagc gtcaagccac cgaagacgac caccagaaca aaagtgatca
     3841 gggccatctt ctcaacctta cgaaagcgaa cccagctata gataagcaca atcgccgtgg
     3901 cgacgatcag cgctgcagta gccgcataga tgtcataaat cttgtaaaac gcgaaaaaga
     3961 caaccagcgg taaaaaatca agaaactgct tcattttacg attccgtaat caaatgctta
     4021 aggttattgg cgaatcaaca tatacaggcg gaacagatag atgagcaata tggcagaaat
     4081 caggttgctc aaggtgttcg ccagtacggc accaatttcc ggggttaatg cggcaaaaga
     4141 agaggcaaaa agcagcaaca gtgtttttgc cagcaaccag ctcagtactg cgggtgccac
     4201 cagacgcata ttcgcccaag tcagccgcat actgctacgc atcgaggcaa aaatgcccat
     4261 tttgtcctgt accagcatca ccggtgccag ggccagtaaa atggccatga taattcccgg
     4321 aacgaccacc agcatgatgc caatctgtac taaaagggta gtcagaaaaa tcagaataaa
     4381 taacttgggc aatatcggcg cactggcacc aatagctcgc agcgcacaga ctctctgacc
     4441 cgcagacacc agctggataa ttaatattac gcctccggcg agaatggcgt taccgattaa
     4501 ttctgaaaaa gtggacgccg ctgaagcctg cagcaaaatt tgttgctgtt ccggtgacat
     4561 attctgaacc aggtcgaaca acccactact gccgctaacg ggcacgccgt cattgagctg
     4621 cgcaagctgt gcatcactgg gtgagaaaac atgccctaac accactgtga taaacgcaca
     4681 tagcaacgat accaacagaa tggtcataaa ttgattacgg aagaaatttc cggtgtcacg
     4741 gtatacggac tgcgccgtga tagacatgca ctctccttga gttttatgca ggtgttaatt
     4801 agcgggcaat tgtaccctgg ttaagcctcg ggtggcagca tcaggtcttg tatggaaaag
     4861 catatctttg taaagcggag gtaattgctg gccttcaggc gtgggcaaac cgcggcaagt
     4921 ggcgatcgag ttaatatttg cgtagagaaa atatttaaaa attgatttaa atcacattaa
     4981 ccaggattct caatgcaact tctaaattaa tccagatcaa taaatggtga attatcatat
     5041 gtaatgtgat ctatgtagga tcatttgtta ctccaatgta ggtatattcg tcacgttttt
     5101 ataaccataa cgacggagcg gatatgaaaa agttaacagt ggcggctttg gcagtaacaa
     5161 ctcttctttc tggcagtgcc tttgcgcatg aagcaggcga attttttatg cgtgcaggtt
     5221 ctgcaaccgt acgtccaaca gaaggtgctg gtagtacgtt aggaagtctg ggtggattca
     5281 gcgtgaccaa taacactcaa ctgggcttga cgtttactta tatggcgacc gacaacattg
     5341 gtgtggaatt actggcagcg acgccgttcc gccataaaat cggcacccgg gcgaccggcg
     5401 atattgcaac cgttcaccat ctgccgccaa cactgatggc gcagtggtat tttggtgatg
     5461 ccagcagcaa attccgtcct tacgttgggg ccggtattaa ctacaccacc ttctttgata
     5521 atggatttaa cgatcatggc aaagaggcgg ggctttccga tctcagtctg aaagattcct
     5581 ggggagctgc cgggcaggtg ggggttgatt atctgattaa ccgtgactgg ttggttaaca
     5641 tgtcagtgtg gtacatggat atcgatacca ccgccaatta taagctaggc ggtgcacagc
     5701 aacacgatag cgtacgcctc gatccgtggg tgtttatgtt ctcagcagga tatcgttttt
     5761 aattccgcac aaaaacgacc ccgtaatata cggggtcaat aaggacatgg tataaagggt
     5821 attatttctt cgcttctacg ccatcagttt cagaacgaat taaaaatttc tcagttgttt
     5881 gcggaatatg ctgaatcagc cagtcggcca tttgcttttc ctcatttaaa atcgcttcga
     5941 tgatcggaat tgagactgta tcaccggcat tttttgctgc tgctaatagt gaggtgtaac
     6001 aggcgatttc aaattgctcg aagacatatc cgctaataga gcctttaact atttcatcag
     6061 aagggaatat accaccgatt gactgcccaa gcgcagccat tttactcatg gaatctttaa
     6121 tgactgaacg tgaaatgtca ttacgatcaa gaatagtttc cagttgaaca atctggtttt
     6181 tggtttcact aagatgttgt tcaatacgag cgcgtagttc aggataatta tctatacggc
     6241 tggccatgga ttcaagcata gattcggctt gcttttccat tgcgtgggcg tcacgtaacc
     6301 agtcatgata atgttcaata cgattcatga aaatgtcctc tttataaaat gactaaaagt
     6361 taaaattcat atttcaggct ttattttcgg ctttcttatt tacgttatta agggccagat
     6421 cagtcagttt gatgtcggtg gccttttctt cttccagggt ttctttcaga agcttcgctg
     6481 ctttacggta acctaattgt tcagccagcg tcgctaatgt cccgtaactg gcaatctcat
     6541 aatgctcgac tttctgtgct gcggcaatca gtgcggcatc acgcacttcg tttttctcgg
     6601 tactttcgat gacctcatta gcttcttcaa taagaccttc cattgccaca catttcatgc
     6661 gcttaatttt cagatttgat tctgattcca caacttgatc aattcgttca atctgtccat
     6721 gagtttcctc gaggtgcgca tgaaaagcct gacttaattt ttcatttgat gttgctcttg
     6781 cgagttttgc cagtgcccgg ggtaattgtt tttctgcgct gtaggtatct gaaagcagtt
     6841 gaataaatac atcttcaatg gtcttcatat tcatattttt ctccagtgaa atcactgcga
     6901 gccatacggc ccgcagatgc aattatcttt atcagctgat attaataatt aacggtgaat
     6961 caggatttgt ctgatttatt accaccgctt tgttgaccgc cttttttacc cgcttcagat
     7021 gcgcgttgcg gatcattttt aaaattaccg ccgctatgct gaccgccttt acggcctgcg
     7081 tcggatgcct tctcacggtc ttcggcgaaa tttcctgaac caccacgatg ttcggccatg
     7141 ttatttctcc cgttgcgttg cattgtttca ttaatatgag tgttgtgtgt cgacactcat
     7201 taaaattagt cgctaatgag aattagtcaa attaagcgca acgagaagat agagggaaaa
     7261 tatattttga ggaacattct ggatatatta acaattacct gaggaataaa tgacttagag
     7321 aaaattattg attttactgg tgttatgttg cgggattaat ttgtttgatt atattactgt
     7381 tgggcggaaa aatgacgtaa gttgacgttc gaccggggta agcgaaacgg taaaaagata
     7441 aatattaaat gaatttagga tttttccggc ttcattaaag aaagttaaaa tgccgccagc
     7501 ggaactggcg gctgtgggat taactgcgcg tcgccgcttt catcggttgt acaaaaactt
     7561 tcagtgccgc cagcattttc tctggctcat taatatgttg ctcgatgatt ttaacaatgg
     7621 ccgaaccaga aatcgcgccc gcagctcctg catcaatcgc tgcttttacc tgatccgggg
     7681 cggaaatacc aaacccctgc aatggaggtg cagcgttgta ctctttcagc ttcgcaacca
     7741 gatgattgag gggtaacgcg gcgcggtttt ctgcgccggt cacgcccgct cgtgacagca
     7801 aatatgtgta accacgaccg taagaggcta tctggcgcag caggtcgtca tcggcatttg
     7861 gcgggcagat gaagataggt gcgacattat ggcgcaacgc ggcctggcgg aagggcgcgg
     7921 actcttcaac tggcacatcg gcaaccagca ccgaatcgac gccgactttc tcgcactggg
     7981 cataaaactc atcaatgcct ttgttaaaca ccagattggc atacatcaac aggccaatgg
     8041 gaatggtcgg gtgtttctgg cgaatcagtg ccagcatttc aaaacattgt gccggagtca
     8101 cacctgccgc aaaggcgcgc agagtggcgt tttgaatggt cgggccatcc gccagtgggt
     8161 cggagaaggg gatacctaac tccagcgcgt cagcaccggc ttcaattagc gtatcgataa
     8221 ttttcaatga ctgctcaatg cccggatcac cgagcgtgac gaaaggaacg aatgcgcctt
     8281 ctttgcgctc cttcaactgg gcaaacagag attcgtagcg ttccatcaga tttcccctcg
     8341 tgctttcaaa atatcgtgaa cggtgaagat gtctttatcg ccgcgaccgg aaaggttaac
     8401 caccagtagc tgctctttat ccgggttttc gcgcatcatt ttcagcgcat gggccagggc
     8461 gtgggaggat tccagcgccg ggatgatccc ttcgtgcagg cacagcgttt tgaaggcttc
     8521 aagggcttca tcatcggtaa tagacacgta atcagcgcgt ccagtgctgt taagatacgc
     8581 gtgttgtggg ccgacagacg ggaaatccag tccggcggag atggagtaag attcttcaat
     8641 ctgcccgtct tcggtttgca tcatcggcgc tttcataccg aaataaatgc ccacgcgacc
     8701 atgttttagc ggtgcgccgt gctcgccagt ttcgataccg tgaccaccag gctccacacc
     8761 aatcaggccg acgttggttt cattgatgaa atcagcaaac atgccgatgg cattcgaacc
     8821 gccgccaaca caggcgataa cggcatccgg caggcgacct tctctttcca gaatctgcgc
     8881 tttggtttct tcgccaatca tccgctgaaa ctcacggaca atggtcggat aaggatgcgg
     8941 gccagccgcg gtgcccagca tatagtgcgc ggtttcataa ctgccggacc agtcgcgtag
     9001 cgcctcatta caggcatctt tcagcgtggc ggaaccgcta tgtaccggga tcacttccgc
     9061 acccattaag cgcatacgaa aaacgttagg cgactggcgt tcaacgtctt tggcacccat
     9121 ataaatacgg catttcaggc cgagcagggc gctggcaagg gccgacgcca cgccatgctg
     9181 accggccccg gtttcggcga tgatttcggt tttacccatc cgcttcgcca gcaacgcctg
     9241 ccccagcacc tggttggttt tatgtgcgcc gccgtgcagc aaatcttcac gcttgagata
     9301 cagcgtggtg ttcgtcccgg ctgtaatgtt ctggcatttg gtcagtgcgg ttggacgccc
     9361 ggcatagttt ttcagcaggt cgttgaactg ggcttgaaat tcaggatctt tttgcgcgct
     9421 gacaaaagct tcttccagct ggcgcagagc aggcatcagg atttgtggca cgtacatgcc
     9481 gccaaactca ccaaaatagg ggttaagtaa tgttgtcatt gttcctttcc ttaatatgcg
     9541 cgcagcgtct ggaaaaccga ggccaaaaga cgtgcgtctt tgatgcccgg ttgcgactct
     9601 acagcagaat taaaatcaag tccggcgcag ccggtttgtg ccgcttccac gcagttatct
     9661 gcgcctaagc cccccgccag cagaacgttg ccaagcgatt gaccatttaa tagtgaccag
     9721 tcaaaacgtt gcccgcttcc accctggccg ttgtctaaaa catatttatc aacgtgctga
     9781 aactcgcggg cgggcagggt ttcaccgacg cttaatgctt tccagatggc aacatgtgct
     9841 ggcagagctt cacgcagcgt atcgatatac agctgatctt cattaccatg cagttgcact
     9901 gccgccagcg ataacacctt agctttgtcc accacatcgg caatatcgtg attgcggaac
     9961 acgccaacat actgcaacgg tgccgcagcc atcacttcct gcgcctgttc aacgttgacg
    10021 caacgcggtg atgtcgcaac aaaaatcaac ccaccgtaaa tcgcgcccgc gtcataagct
    10081 gctttagcat cttgcccacg cgtcaggcca catactttat tctcacccag caacacccgg
    10141 cgcacggcgg cgtgcaaatc gtcatgggcc atcaacgccg aaccaatcag aaaaccgtta
    10201 gcgaagtggc ttaactcgcg cacctgagcg taagtattga tgccggattc gctgattacc
    10261 gttacgttgt gccccagttt cggcgcaagc tcgcgggtac ggttgagatc aatcgacaaa
    10321 tcacgcagat cgcggttgtt gatgccaacg acctttgccc ctaatgcaat ggcgcgctcc
    10381 agttcctctt cattactgac ttcggtcagc acccccatct ccagactgtg agcgacggct
    10441 gcaagctggc gatattggtc gtcatccagt actgaaagca ttaataagca ggcatcggcc
    10501 tggtaatagc gcgccagata gatctggtaa gggtcgataa tgaagtcttt acataaaatc
    10561 ggctgcgggg cgatttggct gacgatgagg aggaaatcaa agctcccctg aaaatatttc
    10621 tcatcagtca gcaccgaaat tgccgaagcg taatgtttat aaatggcggc aatgcgtgcc
    10681 ggatcgaaat catcacggat cacgcctttt gacggcgacg cttttttaca ctccagaata
    10741 aacgccgtgc gtgcaccctg tagcgcatca taaaaatgtc gtgtgctcgg ctgaacctca
    10801 ttctgaaaac tggccagcgg ttgctgctgt ttgcgggttt ctacccaaat cgccttgtct
    10861 gcgacgattt tcgctaaaac ggtttgcatc atttaccctc gtgcagccag cgcggtaact
    10921 ctgtcgtaag cggaaccact gcgcagtacc tcaagaacgg tttgcgcatt ggcttgcaga
    10981 tcttcatggc catgcaggcg cattaacatg gcgacgttcg cagcgacggc tgcttcatgg
    11041 gcggcgtcgc ctttaccttg taacaagcgt gttaaaatgt cacggttttc ttcaggtgtt
    11101 ccgcctgcca gttgctcctg gtggtaggga gtcaggccaa aatcttcagc ggtcaattga
    11161 tagctcttaa ttttgccgtc atgcagctcg gcaacgattg tcggcgcgtg taatgaaact
    11221 tcatccatcc cgccgctgtg caccaccgcc gcgcgttgat accccagcac acgcaaggtt
    11281 tcggcaatcg gcagcaccag ttccggacta taaacaccaa ttaacgccag cggcggatgc
    11341 gccgggttaa tcaatggccc cagcacattg aacagggtgc gggttttcag ttgctggcga
    11401 accggcatcg cgtggcggaa tccggtgtga tatttcggcg caaagaggaa acatacgcct
    11461 aactcgtcca gcgcctggcg cgatttattg gcgttcatat caagattaat accgaatgcc
    11521 gccagcagat ccgacgagcc ggatttactg gagacgctgc ggttaccgtg tttcgccact
    11581 ttcagcccac aggccgcggc gacaaacgca ctggcggtag aaatattgat actgttgctg
    11641 ccgtcaccgc cagtaccgac gatatcagca aacagataat ccgggcgcgg gaacggcgct
    11701 gcgttttcca gtagcgcggt tgctgccccg gcgatctcgt tcgggtgctc accgcgaatt
    11761 ttcatgctca ccagcgccgc cgccagttgt tccggcttca gctcgccacg caccaccgct
    11821 gaaaacagct ggtggctttc ttgttggcta agcgtctgcg cctgatacag tttttccaga
    11881 atcggttgca gcgtgttggc tggctctagt ttctgctgcg cccaggccag cgtttgttcc
    11941 agcaggcgag cgccctgggt ggtgagaatg gattccggat ggaactggaa tccacaaacg
    12001 cgatccgcat cgtgacgtac tgccatcacc atgccattaa aatgggcgtt gatggttaaa
    12061 ccggccggaa tgttactgcc aaccagcgag tgataacgcg ccaccggcag cgggtttgtt
    12121 aatccggcaa acatcgcctg accgtcatgt tcaatgctcg acgctttacc gtgaagaatt
    12181 tcgcccgcct gaccgacata gcccccgtaa gcttcgacaa ttgcctgatg tccgaggcaa
    12241 atgccaataa tgggcagctt gccacgcaag cgggtgagga gttccggcat acaaccggct
    12301 tcgctcggca caccggggcc aggagaaagc atcagtaccg gattgctcat cgtcgccagg
    12361 cgttcaatta aggtctgcgc cggaatatgg ttgcggtaaa tcaccacgtt atgaccattg
    12421 ctgcgcaact gatctgccag gttgtacgta aaagagtcga tattatcgag cagcagaatg
    12481 tcagccatca gaaagtctcc tgtgcatgat gcgcggtggc aatagcgcgc agtacagcgc
    12541 gggctttgtt acgggtttcg tcggcttccg actgcggaac agaatcaagg actacaccag
    12601 cacccgcttg cacggtggcg ataccgtttt ccaccagcgc cgagcggatc acaatgcagg
    12661 tgtcgagatc gccatgcgcg gtgaaataac ctaccgcgcc gccgtagctg ccgcggcgac
    12721 gaccttccgc ctcggcaatt aactgcatag cgcgtacttt cggcgcaccg cttaacgtcc
    12781 ccatattcat acaggcgcga taagcgtgca gggcgtcaag atcgtgacgc agttcgccga
    12841 ctacgcgaga gacgaggtgc atcacatagg aataacggtc aactttggtg agatcggcga
    12901 cgtagcggct gccaggggtg caaatgcgtg ccagatcatt acgggcgaga tcaaccagca
    12961 tcagatgttc agacagctct ttatggtcag tacgcatttc cagttcaata cggctgtcga
    13021 gatctctgtc cagtgaacca tcggcgcgac gaccgcgtgg gcgtgttccg gcaatcgggt
    13081 agatctcaat ctggcggctg gtggcatcat acttgagcga gctttccggc gacgcgccaa
    13141 atagggtgaa atcattatcc tgcataaaaa acatgtacgg gctgggatta ctctttttca
    13201 gcacgtaata ggccgccagc ggtgacgggc agggcagaga gaaacggcga gatggcacca
    13261 cctggaaaat ttctccagcg cgaatcgctt tttgcaacaa acgcactacg ccaccgaact
    13321 cttcatcgct ctgattacat tcacaacgca tatgcggcac ggaaaccact ggcagcggcg
    13381 gcgcggcttc ggtcagttgc tgacgtagtt cgttcaggcg agcagtgaga cgttgttttt
    13441 cttcttcatt cggagcaaac aggctggcct gaatacgggt gctttttttc tgatggtcaa
    13501 tcaccatcag cgtttcagcg agataaaaac agaaatcagg gcagttattt tccgctgaca
    13561 gttgcggtaa atcttcaaat cccgccacaa ggtcataaga gaacaggccg ccgaagaaca
    13621 ttgcttctcg ttcttccttc ggtacattca acagattctg caataaacgg aaagcgtcaa
    13681 aaaccgaaag ggagcataag cgggcgtctt catccagcag tggactgaca ggggggaagc
    13741 gcagcacacg gcagtttggt gattgttcac tttccacacc cgcaggcagg gcgttatcca
    13801 gtagtgccag cagggcttcg ccattgccgg aaagtgcctg aattgtgacg gtgtcaccta
    13861 atgctgtaat gtgcagcgca ctgtctatca gcagcaggct ttttaaatca tctttgctgt
    13921 cgatatctgc ggattccagc agcagcgttg ccggacgatt cccacacaac tggtgaaaaa
    13981 gcgcagtcgg gttgtcgcga taagcgcctt cgcaggttag cagttcgaga gtcggttttt
    14041 gtgtttgcat tgttattctc taattttgtt caaaaaaaag cccgctcatt aggcgggctg
    14101 ggtatctgat tgctttacgc atggtgaata cactgcccgt ttcaggaagt ggccaccaac
    14161 ctttcagtac gaaaattgct ttcattgtcg ataccatttt tacgtgaact tgcgtactag
    14221 tcaactggtt cgatgattaa ttgtcaacag ctcatttcag aatatttgcc agacccgtta
    14281 tgatgtcggc gcaaaaaaca ttatccagaa cgggagtgcg ccttgagcga cacgaattat
    14341 gcagtgattt acgacctgca cagccatacc acagcttccg atggctgcct gacgccagaa
    14401 gcattggtgc accgtgcagt cgagatgcgc gtcggcaccc tggcgatcac cgaccatgac
    14461 accacagctg caatcgcgcc agcaagagag gaaatttcac gttcgggact ggcgctgaat
    14521 cttattcccg gcgtggaaat ttccacggtc tgggaaaatc atgaaattca tattgtcggg
    14581 ctgaatattg atattactca tccgctaatg tgtgagttcc tcgcgcagca gacagaacgg
    14641 cgcaatcagc gggcgcagca aattgccgaa agacttgaaa aagcgcaaat acctggggcg
    14701 ctggaaggcg cacaacgact ggcgcagggc ggcgcagtga cgcgcggtca ttttgcgcgt
    14761 ttccttgtgg agtgcggcaa agccagttca atggcagatg tctttaaaaa gtatctggcg
    14821 cgcgggaaaa ccggatacgt tccgccacag tggtgtacaa tagaacaagc tattgatgtc
    14881 attcatcatt ctggcggtaa ggcggtgcta gctcatcctg ggcggtacaa tcttagtgct
    14941 aaatggctga aaagattggt tgcgcatttt gccgaacacc acggcgacgc gatggaagtc
    15001 gcgcagtgcc agcaatcgcc caatgaacgc acccagctgg ccacacttgc gcgtcagcat
    15061 catttatggg catcacaagg atctgatttt catcagccat gcccgtggat cgaactgggt
    15121 cgtaaactct ggctgcccgc aggcgttgaa ggcgtctggc agctatggga acagccgcag
    15181 aacaccacag agagggaatt atgagccagt ttttttatat tcatcctgat aacccgcagc
    15241 aacgtctgat caaccaggcg gtggagatcg tgcgtaaagg cggggtgatt gtctatccaa
    15301 ctgattccgg ctatgcgctc ggctgtaaaa gtgaagataa aaacgccatg gagcgtattt
    15361 gtcgtattcg ccagctgccg gacggtcaca actttaccct gatgtgtcgc gatctttctg
    15421 aactctcgac ctattcattc gtggataacg ttgcgtttcg tttgatgaaa aacaacacgc
    15481 cgggcaacta taccttcatc ctgaagggga cgaaggaagt gccacgccgt ctgttgcagg
    15541 agaaacgcaa aaccatcggt atgcgtgtgc cttctaaccc gattgcgcag gcgttgcttg
    15601 aagcactggg cgaaccgatg ctttccactt cgctaatgct gccaggcagc gaatttaccg
    15661 aatccgatcc cgaagaaatt aaagaccgtc tggaaaaaca ggtagatttg attattcacg
    15721 gcggctatct cgggcagaaa ccaacaacgg ttattgatct taccgatgat acgccggtcg
    15781 tggtgcgtga aggcgtaggt gatgtgaagc ctttcttata agtgcgtaac tctgtatact
    15841 acgcggcctt aaaatggcgc tatacggcgc cattgattct cctgttcgac gcctgtgaag
    15901 gcgacaccaa aaggaagctc tatgagcgaa aaattacaga aagtgctggc ccgcgctggc
    15961 cacggctccc gccgtgaaat cgaatctatt attgaagccg gtcgtgtgag tgttgatggc
    16021 aaaattgcta aactcggcga tcgcgttgaa gttacccctg ggctgaaaat tcgtatcgat
    16081 ggtcatctga tttcggtacg tgagtccgct gaacaaattt gtcgcgttct ggcctattac
    16141 aaaccggaag gtgagttgtg tacccgtaac gatccggaag ggcgtccgac ggtgtttgac
    16201 cgtctgccaa agctgcgtgg cgcacgctgg attgccgtag gtcgtctgga cgttaacacc
    16261 tgtggtctgc tgctgttcac caccgatggt gaactggcaa accgtttaat gcatccaagc
    16321 cgtgaagttg aacgtgaata cgccgtgcgt gtatttggtc aggttgacga cgcgaaactg
    16381 cgtgatttga gtcgtggcgt gcaactggaa gatggtccgg cagcttttaa aaccatcaag
    16441 ttcagcggcg gcgaagggat caaccagtgg tacaacgtga ctctgaccga aggccgtaac
    16501 cgtgaagttc gtcgtctgtg ggaagcggtt ggtgtgcagg tgagccgcct gatccgtgtt
    16561 cgttacggtg atatcccact gccaaaaggt ctgccacgcg gtggctggac ggagctggac
    16621 ctcgcccaga ctaactatct gcgcgaactg gtggagcttc cgccagaaac cagctctaaa
    16681 gtcgctgtag aaaaagaccg tcgtcgcatg aaggctaatc agattcgtcg tgcggtgaaa
    16741 cgtcacagtc aggtgagcgg cggtcgtcgt tctggcgggc gtaataacgg ttaatcaaag
    16801 tatgccggac gtcatatccg gcatttttac agattaataa tcaatcccta tttgcgcctt
    16861 aacaccggca tcaaacgcat gtctgatggg gcgcagctca cttaccgtat ccgccagttc
    16921 aagaatatcc cgatgacagc ctcgacccgt gataatcacc gtctgttgat gtggacgttc
    16981 atttaacgcc tgcaccactt cttccagtgg caaatagtca tacgccacca tatacgtcag
    17041 ttcatcaagc aaaaccatat ccagtgagga atcagcaagc atccgctttg catgttgcca
    17101 gacttcgcgg caggcggcgg tatcagactc gcggttttgt gtatcccagg taaagcccgt
    17161 tgccatcacc tgaaactcaa cgccatgtgg ctccagcaga ttgcgttcgc cattaggcca
    17221 ggtgccttta ataaactgca cgacgcccac tttttttccg tgaccaactg cgcgtgttgc
    17281 cgtaccaaat gccgcggtgg ttttgccttt tccattgccg gtaaagacga tgataatacc
    17341 gcgttcatcc tgggcctggg ccacacgagc atctactttt tctttcactc gctgctgacg
    17401 ctgttggtag cgttcatcac tcattgggaa attcctggtt tacggcccgg ttgggcgtca
    17461 aaggtcatgc cggttttacg gcggctgtca tcgcccatca gccagaggta gagcggcatg
    17521 atatcagcgg gtgttttaag tttctgtgga tcttcggtcg ggaaggcgct tgcgcgcatt
    17581 gcggtgcgcg taccgcctgg gttaatgcag ttgacgcgca ggcgctgctg atattcatcg
    17641 gccagtacct gcatcatccc ttcggtggca aatttcgacg ctgcatatgc accccagttg
    17701 gctcgtccct gacgtccaac gcttgatgaa gtaaagacca gtgaaccggc gtccgatttg
    17761 agtaataaag gaagcagtgc ctgggtgagc ataaaggtgg cattaacgtt gacctgcatg
    17821 acgtcctgcc agacctgcgg attttgttcg ctcattgggc aaacatcgcc gagcaatccg
    17881 gcattatgca aaacaccatc cagacgcgga taattaacga caatgcgctg tgccagttgt
    17941 tggcaatttt cggacgtgca ggtcagcaaa tcgagaataa accactgtgg ctgacgccca
    18001 gtttcttcgt ttatgtggct ggctacctga cgtaattttt cttcattacg gcccaacaga
    18061 atcactgtcg caccatagcg tgcatacgtc atcgcggctt cacgaccaat cccatcgctg
    18121 gctcccgtca ccaggataat gcgatcattg agtaaatctt gttttggctg gtaatgcatg
    18181 gctactcctc aacgacgttg tctgtcgcgc tgccagtgta ctttatgact ttggggcttt
    18241 atgcctgaaa cagaagggca tttcaatcag cctggggcga taacaccgca aaatggatac
    18301 tttgtcatac tttcgctgca ataacatctc tgcgagacgg cttaacatgc ctgttgtaaa
    18361 ctgtgagcca aagcgttgtt taaccaaggt ggggactcgt ggaattgttg tctgaatatg
    18421 gtttgttttt ggctaaaatc gttaccgttg tgctagcgat tgcggcgatt gccgccatta
    18481 ttgtcaatgt tgctcaacgt aataaacgcc agcgtggcga gttacgggtc aacaatctca
    18541 gcgaacagta taaggagatg aaagaagaac tggccgcggc gctgatggac tcacatcagc
    18601 aaaaacagtg gcacaaagcg cagaagaaaa agcacaagca agaagcgaaa gcagcaaaag
    18661 cgaaagccaa actgggcgag gtggcaactg acagtaaacc tcgcgtctgg gtgctggatt
    18721 ttaaaggcag catggacgcc catgaagtga actcgctacg tgaagagata acggcggtac
    18781 tcgcagcatt caaaccgcag gatcaggttg tgctccgtct ggaaagccct ggtggcatgg
    18841 tgcatggtta cgggttggcg gcttcgcagc tgcagcgtct gcgtgataaa aacattcctt
    18901 taactgttac ggtagacaaa gtcgctgcca gcggcggtta catgatggcc tgtgtggcgg
    18961 acaaaattgt ttccgcaccg tttgctattg tgggttccat tggggtggtg gcgcaaatgc
    19021 ccaactttaa ccgcttcctg aaaagcaaag atattgatat cgaactgcac accgccgggc
    19081 agtataagcg cacgctgacc ttgctgggtg aaaataccga agaagggcgg gagaaattcc
    19141 gcgaagagct gaacgaaacg catcagttat ttaaagattt tgtgaagcgt atgcgtccgt
    19201 ctctggatat tgaacaggtg gcaacgggtg aacactggta cggacaacag gcggtagaga
    19261 aaggcctggt tgatgaaatc aacaccagtg atgaagttat tcttagcctg atggaaggcc
    19321 gtgaagtggt taatgtacgc tatatgcagc gtaaacgact cattgaccga ttcaccggca
    19381 gcgcggcaga gagcgccgat cgattgttgc tacgctggtg gcagcggggt caaaagccat
    19441 tgatgtaaaa gacaaacgcg aggctaagac ctcgcgtttt gctttaatca accagatgat
    19501 atttttctga aagcacatgg gccaggtgtt tgaacatatt aaacaccgcg gtgcttttgg
    19561 ctgttggcaa tccttgttca tctaaaaagt agtcgccggt aaataccagc acgccattac
    19621 gctgcgtgac gccggtggct tcaatccctg cgagcgtatc ctcatgctca cgaatgattt
    19681 tgttagcctc tttcaacagc gtttcgcggt cgatgggttg tgtctctttg ttcattattt
    19741 actccttaaa caaggacatt agtctacgcc aggcatggct tgcagacaaa tataccacgc
    19801 tggtggcaag agcgccttac tggcaacttt ggattttgca tgctaataaa gttgcgtatc
    19861 ggattttatc aggtacagtg tgacgctttc gtcaatctgg caatagattt gcttgacatt
    19921 cgaccaaaat tccgtcgtgc tatagcgcct gtaggccaag acctgttaac tcagtcacct
    19981 gaattttcgt gaacagagtc acgacaaggg ggtgatatcc gcagagagcg agtccatatc
    20041 ggtaactcgt tgccagtgga aggtttatca acgtgcgacg cattcctgga agaatcaaat
    20101 taggtaaggt gaatatgggt aaagctcttg tcatcgttga gtccccggca aaagccaaaa
    20161 cgatcaacaa gtatctgggt agtgactacg tggtgaaatc cagcgtcggt cacatccgcg
    20221 atttgccgac cagtggctca gctgccaaaa agagtgccga ctctacctcc accaagacgg
    20281 ctaaaaagcc taaaaaggat gaacgtggcg ctctcgtcaa ccgtatgggg gttgacccgt
    20341 ggcacaattg ggaggcgcat tatgaagtgt tgcctggtaa agagaaggtc gtctctgaac
    20401 tgaaacaact ggctgaaaaa gccgaccaca tctatctcgc aaccgacctt gaccgcgaag
    20461 gggaagccat tgcatggcac ctgcgggaag tgattggtgg tgatgatgcg cgctatagcc
    20521 gagtggtgtt taacgaaatt actaaaaacg cgatccgcca ggcatttaac aaaccgggtg
    20581 agctgaatat tgatcgtgtt aatgcccagc aggcgcgtcg ctttatggac cgcgtggtgg
    20641 ggtatatggt ttcgccgctg ctgtggaaaa agatcgctcg tggtctgtct gccggtcgtg
    20701 tgcagtcggt ggcggtccgc ctggtggtag agcgtgagcg tgaaattaaa gcgttcgtac
    20761 cggaagagtt ctgggaagtc gatgccagca cgactacgcc atctggtgaa gcgttggcgt
    20821 tgcaggtgac tcatcagaat gacaaaccgt tccgtccggt caacaaagaa caaactcagg
    20881 ctgcggtaag tctgctggaa aaagcgcgct acagcgtgct ggaacgtgaa gacaaaccga
    20941 caaccagtaa acctggcgct ccttttatta cctctacgct gcaacaagct gccagcaccc
    21001 gtcttggttt tggcgtgaaa aaaaccatga tgatggcgca gcgtttgtat gaagcaggct
    21061 atatcactta catgcgtacc gactccacta acctgagtca ggacgcggta aatatggttc
    21121 gcggttatat cagcgataat tttggtaaga aatatctgcc ggaaagtccg aatcagtacg
    21181 ccagcaaaga aaactcacag gaagcgcacg aagcgattcg tccttctgac gtcaatgtga
    21241 tggcggaatc gctgaaggat atggaagcag atgcgcagaa actgtaccag ctaatctggc
    21301 gtcagttcgt tgcctgccag atgaccccag cgaaatatga ctccacgacg ctgaccgttg
    21361 gtgcgggcga tttccgcctg aaagcacgcg gtcgtatttt gcgctttgat ggctggacaa
    21421 aagtgatgcc tgcactgcgt aaaggcgatg aagatcgtat cttaccggca gtcgataaag
    21481 gcgatgctct gacgctcgtt gaactaacac cagcccagca ctttaccaag ccgccagccc
    21541 gtttcagtga agcatcgctg gttaaagaac tggaaaaacg tggtatcggt cgtccgtcta
    21601 cctatgcgtc gatcatttcg accattcagg atcgtggcta tgtgcgagta gaaaatcgtc
    21661 gtttctatgc ggaaaaaatg ggcgaaatcg tcaccgatcg ccttgaagaa aatttccgcg
    21721 agttaatgaa ctacgacttt accgcgcaga tggaaaacag cctcgaccag gtggcaaatc
    21781 acgaagcaga gtggaaagct gtactggatc acttcttctc ggatttcacc cagcagttag
    21841 ataaagctga aaaagatccg gaagagggtg gtatgcgccc gaaccagatg gttctgacca
    21901 gcattgactg cccgacttgt ggtcgcaaaa tggggattcg cacagcgagc accggggtat
    21961 tccttggctg ttctggctat gcgctgccgc cgaaagagcg ttgcaaaacc accattaacc
    22021 tggtgccgga aaacgaagtg ctgaacgtgc tggaaggcga agatgctgaa accaacgcgc
    22081 tgcgcgcaaa acgtcgttgc ccgaaatgcg gcacggcgat ggacagctat ctcatcgatc
    22141 cgaaacgtaa gttgcatgtc tgtggtaata acccaacctg cgacggttac gagatcgaag
    22201 agggcgaatt ccgcattaaa ggttatgacg gcccgatcgt tgagtgtgaa aaatgtggct
    22261 ctgaaatgca cctgaaaatg gggcgtttcg gtaaatacat ggcctgcacc aacgaagagt
    22321 gtaaaaacac acgtaagatt ttacgtaacg gcgaagtggc accaccgaaa gaagatccgg
    22381 tgccattacc tgagctgccg tgcgaaaaat cagatgctta tttcgtgctg cgtgacggtg
    22441 ctgccggtgt gttcctggct gccaacactt tcccgaaatc gcgtgaaacg cgtgcgccac
    22501 tggtggaaga gctttatcgc ttccgcgacc gtctgccgga aaaactgcgt tatctggccg
    22561 atgcgccaca gcaggatccg gaaggtaata agaccatggt tcgctttagc cgtaaaacca
    22621 aacagcaata tgtctcttcg gaaaaagacg gaaaggcgac tggctggtca gcattttatg
    22681 ttgatggcaa atgggttgaa ggaaaaaaat aacctttaat tctgtcaggt ttttataaac
    22741 aaagggtcgc gaaagcggcc cttttttatg gcatattatt ttttcttcac acctatacac
    22801 taaggctata aatgatatag tggttatagt tagcaccttt tttattatta aatcgtatta
    22861 gtcacccgcc aggtgtgacg aaaaaacgat gttctgatgg cgtctaagtg gatggtttaa
    22921 catgaaatta caacaacttc gctatattgt tgaggtggtc aatcataacc tgaatgtctc
    22981 atcaacagcg gaaggacttt acacatcaca acccgggatc agtaagcaag tcagaatgct
    23041 ggaagacgag ctaggcattc aaattttttc ccgaagcggc aagcacctga cgcaggtaac
    23101 gccagcagga caagaaataa ttcgtatcgc tcgcgaagtc ctgtcgaaag tcgatgccat
    23161 aaaatcggtt gccggtgagc acacctggcc ggataaaggc tcgctgtata tcgccaccac
    23221 gcatacccag gcacgctacg cgttaccaaa cgttatcaaa ggctttattg agcgttatcc
    23281 ccgcgtttct ttgcatatgc accagggccc gccgacacaa attgctgatg ccgtctctaa
    23341 aggcaatgcc gatttcgcta tcgccacaga agcgctgcat ctgtatgaag atttagtgat
    23401 gttaccgtgc taccactgga atcgggctat tgtagtcact ccggatcacc cgctggcagg
    23461 caaaaaagcc attaccattg aagaactggc gcaatatccg ttggtgacat ataccttcgg
    23521 ctttaccgga cgttcagaac tggatactgc ctttaaccgc gcagggttaa cgccgcgtat
    23581 cgttttcacg gcaacggatg ctgacgtcat taaaacttac gtccggttag ggctgggggt
    23641 aggggtcatt gccagcatgg cggtggatcc ggtcgccgat cccgaccttg tgcgcgttga
    23701 tgctcacgat atcttcagcc acagtacaac caaaattggt tttcgccgta gtactttttt
    23761 gcgcagttat atgtatgatt tcattcagcg ttttgcaccg catttaacgc gtgatgtcgt
    23821 tgatgcggct gtcgcattgc gctccaatga agaaattgag gtcatgttta aagatataaa
    23881 actgccggaa aaataatttc ttgcgttatt ttcggcacct tttatgtagc gaaggtgccg
    23941 gaatatattc tcttttgtta cttatttcta taccgtgcac aatattttta tccctcatat
    24001 ttatagggta aattacctgt aaagtagtgt ctaattaccc gcctcgcaaa ttccagtttt
    24061 caaatatcta tttcaggtca aaagattgaa ttattcactg gagacgattc gtaaattcgc
    24121 tggatttttg actaaagttt ctttaggatt tgtctcatcg tatgattaat tacaccaatc
    24181 attcaacgtc ttgctgataa gtgatggcga ttatatgagg ttagcaatgc cttctggaaa
    24241 tcaggaaccc cgccgtgatc ctgaattaaa acgtaaagcc tggctggcgg tttttcttgg
    24301 ttctgcactt ttctgggtgg ttgtcgcact gctgatttgg aaagtgtggg gataactatg
    24361 gtcggtcagg agcaactgga gtcgtcacca ttatgccagc atagtgacaa tgaaccagaa
    24421 acgaaacggg aatgttccgt cgttattcca gacgaccggc aactaacatc gcagcagcaa
    24481 gcctttatag aactgtttgc tgaagatgat cagccgaaac aataattatc atcattctta
    24541 ttacccattt ttaatgaatt aaagggcttt taatacaccg cagcaataac agcttgagtt
    24601 atctcaacac aaaataataa ccgttaaggg tgtagcctat gatcaacaca aatatgaaat
    24661 attggtcctg gatgggcgcg ttttctctgt cgatgctctt ctgggccgaa ctcctctgga
    24721 tcattactca ctgatccttg accccgctgc ggcggggttg tcatttgctt tgccacaagg
    24781 tttctcctct tttatcaatt tgggttgtta tcaaatcgtt acgcgatgtt tgtgttatct
    24841 ttaatattca ccctgaagag aatcagggct tcgcaaccct gtcattaagg aggagctatg
    24901 tcgtcaaccc tacgagaagc cagtaaggac acgttgcagg ccaaagataa aacttaccac
    24961 tactacagcc tgccgcttgc tgctaaatca ctgggcgata tcacccgtct acccaagtca
    25021 ctcaaagttt tgctcgaaaa cctgctgcgc tggcaggatg gtaactcagt taccgaagag
    25081 gatatccacg cactggcggg atggctgaaa aatgcccatg ctgaccgtga aattgcctac
    25141 cgcccggcaa gggtgctgat gcaggacttt accggcgtac ctgccgttgt tgatctggcg
    25201 gcaatgcgcg aagcggttaa acgcctcggt ggcgatactg caaaggttaa cccgctctca
    25261 ccggtcgacc tggtcattga ccactcggtg accgtcgatc gttttggtga tgatgaggca
    25321 tttgaagaaa acgtacgcct ggaaatggaa cgcaaccacg aacgttatgt gttcctgaaa
    25381 tggggaaagc aagcgttcag tcggtttagc gtcgtgccgc caggcacagg catttgccat
    25441 caggttaacc tcgaatatct cggcaaagca gtgtggagcg aattgcagga cggtgaatgg
    25501 attgcttatc cggatacact cgttggtact gactcgcaca ccactatgat caacggcctt
    25561 ggcgtgctgg ggtggggcgt gggtgggatc gaagcagaag ccgccatgtt aggccagccg
    25621 gtttccatgc ttatcccgga cgtagtgggc tttaaactta ccggaaaatt acgtgaaggt
    25681 attaccgcca cagacctggt tctcactgtt acccaaatgc tgcgcaaaca tggcgtggtg
    25741 gggaaattcg tcgaatttta tggtgatggt ctggattcac taccgttggc ggatcgcgcc
    25801 accattgcca atatgtcgcc agaatatggt gccacctgtg gcttcttccc aatcgatgca
    25861 gtaaccctcg attacatgcg tttaagcggg cgcagcgaag atcaggtcga gttggtcgaa
    25921 aaatatgcca aagcgcaggg catgtggcgt aacccgggcg atgaaccgat ttttaccagt
    25981 atgttagaac tggatatgaa tgacgttgaa gcgagcctgg cagggccaaa acgcccacag
    26041 gatcgcgttg cactgcccga tgtaccaaaa gcatttgccg ccagtaacga actggaagtg
    26101 aatgccacgc ataaagatcg cctgccagtc gattatgtta tgaacggaca tcagtatcag
    26161 ttacctgatg gcgctgtggt cattgctgcg ataacctcgt gcaccaacac ctctaaccca
    26221 agtgtgctga tggccgcagg cttgctggcg aaaaaagccg taactcttgg cctcaagcgg
    26281 caaccgtggg tcaaagcgtc gctggcaccg ggttcgaaag tcgtttctga ttatctggca
    26341 aaagcgaaac tgacacagta tctcgacgaa ctggggttta accttgtggg atacggttgt
    26401 accacctgta ttggtaactc tgggccgctg cccgatccta tcgaaacggc aatcaaaaaa
    26461 ggcgatttaa ccgtcggtgc ggtgctgtcc ggcaaccgta actttgaagg ccgtatccat
    26521 ccgctggtta aaactaactg gctggcctcg ccgccgctgg tggttgccta tgcgctggcg
    26581 ggaaatatga atatcaacct ggcttctgag cctatcggcc atgatcgcaa aggcgatccg
    26641 gtttatctga aagatatctg gccatcggca caagaaattg cccgtgcggt agaacaagtc
    26701 tccacagaaa tgttccgcaa agagtacgca gaagtttttg aaggcacagc agagtggaag
    26761 gaaattaacg tcacacgatc cgatacctac ggttggcagg aggactcaac ctatattcgc
    26821 ttatcgcctt tctttgatga aatgcaggca acaccagcac cagtggaaga tattcacggt
    26881 gcgcggatcc tcgcaatgct gggggattca gtcaccactg accatatctc tccggcgggc
    26941 agtattaagc ccgacagccc agcgggtcga tatctacaag gtcggggtgt tgagcgaaaa
    27001 gactttaact cctacggttc gcggcgtggt aaccatgaag tgatgatgcg cggcaccttc
    27061 gccaatattc gcatccgtaa tgaaatggtg cctggcgttg aaggggggat gacgcggcat
    27121 ttacctgaca gcgacgtagt ctctatttat gatgctgcga tgcgctataa gcaggagcaa
    27181 acgccgctgg cggtgattgc cgggaaagag tatggatcag gctcaagccg tgactgggcg
    27241 gcaaaaggac ctcgtctgct tggaattcgt gtggtgattg ccgaatcgtt tgaacgaatt
    27301 caccgttcga atttaattgg catgggcatc ctgccgctgg aatttccgca aggcgtgacg
    27361 cgtaaaacgt tagggctaac tggggaagag aagattgata ttggcgatct gcaaaatcta
    27421 caacctggcg cgacggttcc ggtgacgctt acgcgcgcgg atggtagcca ggaagtcgta
    27481 ccctgccgtt gtcgtatcga caccgcgacg gagttgacct actaccagaa cgacggcatt
    27541 ttgcattatg tcattcgtaa tatgttgaag taacaagtat ttgcttgccg gttattttgc
    27601 ttccggcaag caaatgaatt acacaatgca agagggttat ttgttcagca aatggcccat
    27661 tttctcggct ttggtatcga gataatgttc gttattgggg ttacgaccta caatcaatgg
    27721 tacgcgttca acaatattaa tccctgcttc ggtcagaatt tcgacttttt tcgggttatt
    27781 ggttaacaag cggacttcat tgacgccaag gagtttgaac atatcagcgc aaagagtgaa
    27841 gtcgcgctca tcagcggcga agcctaactg gtggttagcc tctacggtat cgtaaccttg
    27901 atcctgcagt gcgtaagcgc ggattttatt cagcagacca atgttacgac cttcctgacg
    27961 gtgatacagc aaaataccac ggccttcctc ggcaatttgc gtcaatgccg cttcgagctg
    28021 gaagccacaa tcgcagcgca agctgaacag ggcgtcaccg gtcagacatt cggaatggac
    28081 gcgcgcaagt accggggtat gcccggaaat atcgccatag actagcgcga catgatcgtg
    28141 tccggttgcc agttcttcaa atcccaccat caggaaatcg ccccatgggg ttggcagttt
    28201 ggcttctgcc acacgtttaa gctgcatgaa attctccaga taatgctggt tctgtattgg
    28261 cttattttgc cataacgaga aggggttcac ctaatcacga cgcgtcgatc gttcacggaa
    28321 tggcacaaat ctgtcaattt ttcttggaac tggcattttc agttatgatt gtgggactta
    28381 tcaaaaagga gaggccatgc gttcgattgc ccgacgtacc gcagtgggag ctgcactatt
    28441 gtttgtcatg ccagtagccg tatggatttc tggctggcgt tggcaacctg gagaacaaag
    28501 ttggcgacta aaagcggctt tttgggttac tgaaactgtc acccagccct ggggcgtcat
    28561 tacacatttg attttgttcg gctggtttct ctggtgtctg cgttttcgca ttaaggctgc
    28621 ctttgtatta ttttccattc tggcggcggc aatccttgtt ggacaaggcg ttaaatcctg
    28681 gatcaaagac aaagtacagg aaccacgacc ttttgttatc tggttggaaa aaacacatca
    28741 tattccggtt gatgagttct acactttaaa gcgagcagaa cgcggaaatc tagtaaaaga
    28801 acagttggct gaagagaaaa atattccaca atatttgcgt tcacactggc agaaagagac
    28861 ggggtttgcc tttccttccg gtcacacgat gtttgctgcc agttgggcac tgctggccgt
    28921 tggtttgctg tggccgcgtc ggcgaacgtt aaccattgct atcttgttgg tctgggcaac
    28981 gggagtcatg ggaagccgcc tgctactcgg gatgcattgg ccacgcgatc tggtggtagc
    29041 tacgttgatt tcgtgggcgc tggtggcggt ggcaacgtgg cttgcgcaac gaatttgtgg
    29101 gccattaaca ccacctgcgg aagaaaatcg cgaaattgca caacgagaac aagaaagtta
    29161 acgctggttg attttccgaa tttaggcctt aaatcatcaa caatgcgcgt ggatgccatt
    29221 tcgcagacgg cgcgaaaatg gtactttaaa gggctattgc ggtaagttga ccataattta
    29281 ttcgctctaa ccacataacg ggaagtaatg tgaaatattt actcattttc ttactggtgt
    29341 tagcgatctt cgtgatttcg gtcacgttgg gtgcgcagaa cgatcaacag gtgacgttta
    29401 attatctgtt agcgcaaggg gagtaccgta tttccacatt gctggcggta ttgtttgctg
    29461 cggggtttgc tatcggttgg ttgatttgtg gcctgttctg gctgcgagtt cgtgtttccc
    29521 tggcgcgcgc tgaacgtaaa ataaagcgac tggaaaacca gctttcaccc gcgactgacg
    29581 tggctgtagt gccgcactcg tcagcggcga aggaataact ttctatgctg gagttgttgt
    29641 ttctgctttt gcctgtagcc gctgcctatg gctggtatat gggccgcaga agtgcgcaac
    29701 aaaacaagca agatgaagcc aaccgcttgt cgcgtgatta cgtagcgggg gttaacttcc
    29761 tgcttagtaa tcaacaggat aaagcggtag acctgtttct cgatatgctt aaagaggata
    29821 caggcaccgt tgaagcccac cttacgctcg gaaacctgtt ccgttcgcgt ggcgaagttg
    29881 atcgcgctat tcgcatccat cagaccctaa tggaaagcgc ctcgctgacc tatgaacagc
    29941 gtctgttggc gattcaacaa ctggggcgtg attacatggc cgccgggtta tacgaccgcg
    30001 cggaagacat gttcaatcag ctgaccgatg aaactgactt ccgcattggc gcgctgcaac
    30061 agttgctaca aatctaccag gctaccagtg agtggcagaa agcaattgat gttgccgaac
    30121 gcctggtgaa gctgggtaaa gataaacagc gcgtcgaaat tgcccatttc tactgtgagt
    30181 tagccctgca gcatatggcc agcgacgatc tcgatcgtgc catgaccttg ctaaaaaaag
    30241 gggcggcggc agataaaaac agcgcccgcg tatccatcat gatgggacgc gtgtttatgg
    30301 cgaaaggaga atacgccaaa gccgtcgaaa gtttgcaacg cgtcatatcc caggacagag
    30361 aactggtcag cgaaacgctg gaaatgttgc aaacctgcta tcagcagttg ggtaaaactg
    30421 ccgaatgggc agaattcctg caacgcgcgg tggaagagaa caccggtgcc gatgctgaat
    30481 tgatgcttgc tgatatcatc gaagcgcgcg acggtagtga ggctgcacag gtctatatta
    30541 cacgccagct tcagcgtcat ccgaccatgc gtgtattcca taagctaatg gattaccact
    30601 taaatgaagc ggaagaaggg cgcgccaaag agagtctgat ggtgctgcgt gacatggttg
    30661 gcgagaaggt gcgtagtaag cctcgttata gctgccagaa atgtggtttt accgcataca
    30721 ccctctactg gcattgtccg tcttgtcgtg cctggtcaac tattaaaccg attcgcggtc
    30781 ttgatggcct gtaattttta aaaaaatccg actttagtta caacatacta gttattgatg
    30841 tcccattgtg ctccggcaac gacggagcag aaaaagcctg ctaggggaac aatcgcaact
    30901 gttaattttt tatttccacg ggtagaatgc tcgccgttta cctgtttcgc gccacttccg
    30961 gtgcccatca tcaagaaggt ctggtcatga cgttaactgc ttcatcttct tcccgcgctg
    31021 ttacgaattc tcctgtggtt gttgcccttg attatcataa ttgtgatgac gcgctgtcct
    31081 ttgtcgacaa gatcgaccca cgcgattgtc gtctgaaggt cggtaaagag atgtttacat
    31141 tgtttgggcc acagtttgtg cgcgaacttc aacagcgtgg ttttgatatc tttcttgacc
    31201 tgaaattcca cgatatcccc aacactgcag cgcacgctgt cgctgctgca gctgacttag
    31261 gcgtgtggat ggtgaatgtt catgcctccg gtggggcgcg tatgatgacc gcagcgcgtg
    31321 aggcactggt tccgtttggc aaagatgcac cgcttttgat tgctgtgaca gtgttgacca
    31381 gcatggaagc cagcgacctg gccgatcttg gcgtgacact gtcacctgca gattatgcag
    31441 aacgtctggc agcactgacg caaaaatgtg gccttgatgg tgtggtgtgt tctgctcagg
    31501 aagctgtgcg ctttaaacag gtattcggtc aggagttcaa actggttaca ccgggcatcc
    31561 gtccgcaggg gagtgaagct ggtgaccagc gccgcattat gacgccagaa caggcgttgg
    31621 cggctggtgt tgattatatg gtgattggtc gcccggtaac gcaatcggta gatccagcgc
    31681 agacactgaa agcgatcaac gcctctttac agcggagtgc atgatgagtg attccaacag
    31741 ccgtctggtc tactcaacgg aaaccgggcg tattgatgag cccaaagcgg ccccagtacg
    31801 ccctaaaggc gatggagcgg tgcgtattca gcgtcagacc agtggacgta aaggtaaggg
    31861 cgtttgcctg attaccggta tcgatctcga tgatgccgaa ctgacaaaac ttgcagcgga
    31921 actgaagaaa aaatgcggct gcggcggagc agttaaagat ggagttattg aaatccaggg
    31981 cgataagcgt gatttattaa aatcactact tgaagcgaaa gggatgaagg taaaactcgc
    32041 aggcggttaa cataaaaagc cacggatata tccgtggctt tcgaatattt tactgtgtgt
    32101 attattcagt tatttcgtac tgaacagaa
//
LOCUS       DADMEQ010000037        31295 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.037, whole genome shotgun sequence.
ACCESSION   DADMEQ010000037 DADMEQ010000000
VERSION     DADMEQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.037"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            7..1025
                     /locus_tag="JNZ05_001729"
                     /pseudo
     CDS             7..1025
                     /locus_tag="JNZ05_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342299.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(1107..1445)
                     /gene="rcnB"
                     /locus_tag="JNZ05_001730"
     CDS             complement(1107..1445)
                     /gene="rcnB"
                     /locus_tag="JNZ05_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HAY8564995.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1664..2500)
                     /locus_tag="JNZ05_001731"
     CDS             complement(1664..2500)
                     /locus_tag="JNZ05_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HAY8564996.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTV
                     FWMFWRTWRGERNWLENMHGHDYEHHHHHHDHEHHQDHEHHHDQGHHHHHEHGEYQDA
                     HANDIKRRFDGREVINWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFS
                     IGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGI
                     MR"
     gene            2621..2893
                     /gene="rcnR"
                     /locus_tag="JNZ05_001732"
     CDS             2621..2893
                     /gene="rcnR"
                     /locus_tag="JNZ05_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HAY8564997.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      2985..3081
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3116..3904
                     /gene="thiM"
                     /locus_tag="JNZ05_001733"
     CDS             3116..3904
                     /gene="thiM"
                     /locus_tag="JNZ05_001733"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HAY8564998.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            3901..4701
                     /gene="thiD"
                     /locus_tag="JNZ05_001734"
     CDS             3901..4701
                     /gene="thiD"
                     /locus_tag="JNZ05_001734"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAY8564999.1"
                     /translation="MKRINALTIAGTDPSGGAGILADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            4766..5584
                     /locus_tag="JNZ05_001735"
     CDS             4766..5584
                     /locus_tag="JNZ05_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HAY8565000.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVDELQAFVDGIKETP"
     gene            5636..6389
                     /locus_tag="JNZ05_001736"
                     /pseudo
     CDS             5636..6389
                     /locus_tag="JNZ05_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(6363..7328)
                     /locus_tag="JNZ05_001737"
     CDS             complement(6363..7328)
                     /locus_tag="JNZ05_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY8565001.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIRAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(7325..8328)
                     /locus_tag="JNZ05_001738"
                     /pseudo
     CDS             complement(7325..8328)
                     /locus_tag="JNZ05_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(8325..8801)
                     /locus_tag="JNZ05_001739"
     CDS             complement(8325..8801)
                     /locus_tag="JNZ05_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8565002.1"
                     /translation="MLARSSFHRKGIQKVLLCGIVIAYLTLPVNFTYALLFLGILLHG
                     VSYDFYYVTAYIYVDKKAPVHMRTAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQE
                     PVNGLTFNWSGMWTFGAVMIAIIAVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            8955..10007
                     /gene="fbaB"
                     /locus_tag="JNZ05_001740"
     CDS             8955..10007
                     /gene="fbaB"
                     /locus_tag="JNZ05_001740"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HAY8565003.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVAVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            10315..11169
                     /gene="gatY"
                     /locus_tag="JNZ05_001741"
     CDS             10315..11169
                     /gene="gatY"
                     /locus_tag="JNZ05_001741"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HAY8565004.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRLKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNLAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            11198..12460
                     /gene="gatZ"
                     /locus_tag="JNZ05_001742"
     CDS             11198..12460
                     /gene="gatZ"
                     /locus_tag="JNZ05_001742"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HAY8565005.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAVVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            12470..12922
                     /gene="gatA"
                     /locus_tag="JNZ05_001743"
     CDS             12470..12922
                     /gene="gatA"
                     /locus_tag="JNZ05_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HAY8565006.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            12953..13237
                     /gene="gatB"
                     /locus_tag="JNZ05_001744"
     CDS             12953..13237
                     /gene="gatB"
                     /locus_tag="JNZ05_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HAY8565007.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            13241..14597
                     /locus_tag="JNZ05_001745"
                     /pseudo
     CDS             13241..14597
                     /locus_tag="JNZ05_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
     gene            14645..15685
                     /gene="gatD"
                     /locus_tag="JNZ05_001746"
     CDS             14645..15685
                     /gene="gatD"
                     /locus_tag="JNZ05_001746"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY8565008.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLDPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            15785..16564
                     /locus_tag="JNZ05_001747"
     CDS             15785..16564
                     /locus_tag="JNZ05_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY8565009.1"
                     /translation="MTMNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRF
                     LEQKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMT
                     VILDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMH
                     GSIAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKF
                     NRRGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(16646..17545)
                     /gene="yegS"
                     /locus_tag="JNZ05_001748"
     CDS             complement(16646..17545)
                     /gene="yegS"
                     /locus_tag="JNZ05_001748"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HAY8565010.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            17951..18268
                     /locus_tag="JNZ05_001749"
     CDS             17951..18268
                     /locus_tag="JNZ05_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565011.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(18610..19971)
                     /locus_tag="JNZ05_001750"
     CDS             complement(18610..19971)
                     /locus_tag="JNZ05_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HAY8565012.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEVMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(20118..20440)
                     /locus_tag="JNZ05_001751"
                     /pseudo
     CDS             complement(20118..20440)
                     /locus_tag="JNZ05_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
     gene            complement(20631..21353)
                     /gene="baeR"
                     /locus_tag="JNZ05_001752"
     CDS             complement(20631..21353)
                     /gene="baeR"
                     /locus_tag="JNZ05_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HAY8565013.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHESGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(21350..22753)
                     /gene="baeS"
                     /locus_tag="JNZ05_001753"
     CDS             complement(21350..22753)
                     /gene="baeS"
                     /locus_tag="JNZ05_001753"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HAY8565014.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLIDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(22750..24165)
                     /locus_tag="JNZ05_001754"
     CDS             complement(22750..24165)
                     /locus_tag="JNZ05_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8565015.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMPLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(24166..27231)
                     /gene="mdtC"
                     /locus_tag="JNZ05_001755"
     CDS             complement(24166..27231)
                     /gene="mdtC"
                     /locus_tag="JNZ05_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HAY8565016.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLRITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(27232..30354)
                     /gene="mdtB"
                     /locus_tag="JNZ05_001756"
     CDS             complement(27232..30354)
                     /gene="mdtB"
                     /locus_tag="JNZ05_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HAY8565017.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPSASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLNVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKSLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLVLWTK
                     SRFARHEEEA"
     gene            complement(30354..>31295)
                     /locus_tag="JNZ05_001757"
     CDS             complement(30354..>31295)
                     /locus_tag="JNZ05_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HAY8565018.1"
                     /translation="TGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDP
                     SQFKVALAQTQGQLVKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETEGT
                     IKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDL
                     VFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKA
                     RFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKHLV
                     TPGIQDSQKVEVVEAQSATTPEEKATSREYAKKGARS"
BASE COUNT         7702 a         7894 c         8208 g         7491 t
ORIGIN      
        1 cgcataatgc tatttatatt aatgatgatg gttatgcctg tgagctatgc ggcatgttat
       61 agtgagttat ctgttcagca caatttggtt gttcagggga attttgcact tactcaaaca
      121 caaatagcga catatgagca taattttaat gattcgtcat gcgtgagtac aaatactatc
      181 acccctatga gcccatcgga tattattgtt ggactttata acgataccat taaattaaat
      241 ttacattttg aatggaccaa taaaaacaac atcacgttgt caaataatca gagcagtttc
      301 accagtggtt attcggtaac ggtgacacct gcggccagta atgcaaaagt gaatatttct
      361 gcggggggcg gtggttcagt gatgattaat ggtgttgcga cattatccag tgcttcatca
      421 tcgacacgcg ggagtgccgc agtacaattt ctactgtgtt tattaggtgg caagtcatgg
      481 gatgcatgtg taaatagcta cagaaatgca ttggcacaaa atgcaggtgt ctattccttc
      541 aatctgacat tgtcatacaa cccgataacc acaacctgca aacaggacga tttattaatt
      601 actttagaca gtattcccgt ttcacaatta ccagccacag gtaacaaagc aacaataaat
      661 agtaaaaaaa gggatattat tctgcgttgt aaaaatttat taggtcaaca aaatcaaaca
      721 tcacggaaaa tgcaggtgta tttatcaagt tctgacttgt taaccaacag cgacacaata
      781 ctgaaaggtg cggaagataa tgcgtaggat ttattcttga aagtaatggt tcgccagtca
      841 cacttttaaa tatcactaac agcagtaaag gatacacaaa tttaaaagaa attgcggcaa
      901 agtcaaaact tacagataca acggtttcaa ttccgataac agccagttac tacgtctacg
      961 atacaaacaa agttaaatct ggcgcactgg aggcaaccgc attaatcaac gtgaaatacg
     1021 actaataaca agcaagacga gcaagtggct aataataaaa aataacaagg tgtctgcatt
     1081 tccccctcat gatgaggggg ctttttttag cgatgataaa aaatctcacc gtcgtaggct
     1141 ttaatgattt taccgtcggt gtcgctgatc aacacgtacg cgccacccat ataggtccag
     1201 tgcgtcccgg catcaggcgc gggcaggtta cgtaactgcc actgcttaat gttgtactca
     1261 tcggtgcggt acatctctgg gacggtatca cctggtttga aatgcttaaa gtcagcaatg
     1321 aaactactta actcatactt agaaattccc gaggtattgg tcgaacccgc ggtggctggt
     1381 gcggcccagg cggcactggt aaccagcaaa agcacaccca gcaacatctt atttttaatg
     1441 gtcataaact ctccactttg gggatctgta atatcagtcg catatctttc ctgccataca
     1501 atataactga caagcgatat tttgttagtg atcccatgat ttcgtaaaaa gtgtaaatga
     1561 aaggatgccc taaaagcatc cttagcgagc agagagtcag cggaaatagc tgatttaaga
     1621 atcatcctgg gctgaagata acatgcaagc ctaaaggatt gagttatcgc attatgccca
     1681 tgaagccatg tacgcccata tacacaccga ctaagccaat caacagactg gaaaaatagg
     1741 gggcgcgttt agcgagagtg ttaaatccgc tccagcgttt tgcgacctgc tgaacgctga
     1801 ttgctgcgcc aacgcctacg gtgacaagcg ttaacgccag gccaatgctg aaactgacga
     1861 ccagtgttgc gcccagtgtc agggctttca actgaatgca tatcaacagc acggtaattg
     1921 ctgccgggca ggggataagg ccaccggtta agccaaataa caaaatttgc cagttgatga
     1981 cctctctacc atcaaagcgt cgtttaatgt cattggcatg ggcatcctga tactcgccat
     2041 gttcgtgatg gtgatgatgt ccttggtcgt ggtgatgttc gtggtcttga tgatgttcgt
     2101 gatcgtgatg atggtgatga tgctcataat catgcccgtg catattctcc agccagttgc
     2161 gttcgccgcg ccaggtacgc cagaacatcc agaacacggt gccaataatg atcactgcgg
     2221 aaatcagctg gagccacggt tctgctgatt gagcagtaaa gcgcttactg atcaccatcc
     2281 cgccaaaggc aatcaaccag accaccgcgg tatgcgaaat ggttgctgcc agtccgagca
     2341 tcaccgcctg tttaatggtg cctttgatgg cgatgataaa cgccgccatc atcgtttttg
     2401 agtgccctgg ttccaggcca tgaagcgcac caagtaagat ggcgctgggg atgaagaacc
     2461 aggcgtttcc ttgctgaaga agagttgtaa attcggtcat gataataatt cttagtatta
     2521 attcggcaat ctgattctac tcccccccag tacctgatac taccccccag tagattaata
     2581 gtgctatgat ttttcatatt cttgttaacc aggtgttgcc atgtctcata caatccgtga
     2641 taaacagaaa ctgaaagcgc gtgccagtaa gattcagggc caggtcgtgg cgctcaagaa
     2701 aatgctcgac gagccgcacg aatgcgctgc agttttacaa cagattgctg ctatccgtgg
     2761 agcggtaaac ggtctgatgc gggaagtgat taaaggtcat ctgacggaac acatcgttca
     2821 ccagggggat gagctaaaac gtgaagaaga tctggatgtc gttctgaagg tgctggattc
     2881 atatatcaaa taatttatta acgcgattgt aaaactgccg tttttcctcg tttacaacgg
     2941 gtgcgctgga cattaccatc ctcctctgcg atttatcatc gcaaccaaac gactcggggt
     3001 gcccttctgc gtgaaggctg agaaataccc gtatcacctg atctggataa tgccagcgta
     3061 gggaagtcac ggaccaccag gtcattgctt cttcacgtta tggcaggagc aaactatgca
     3121 agtcgacctg ctgagttcag cgcaatctgc gcacgcgtta cacctttttc accaacattc
     3181 ccctcttgtg cactgcatga ccaatgatgt ggtgcaaacc tttaccgcca ataccttgct
     3241 ggcgctcggt gcatcgccag cgatggttat cgaaaccgaa gaggccagtc agtttgcggc
     3301 tatcgccagt gccttgttga ttaacgttgg cacactgacg cagccacgcg cacaggcgat
     3361 gcgtgctgcc gttgagcaag caaaaagctc tcaaacacgc tggacgcttg atccagtagc
     3421 ggttggtgcg ctcgattatc gccgccattt ttgtcatgaa cttttatctt ttaaaccggc
     3481 agcgatacgt ggtaatgctt cggaaatcat ggcattagct ggcattgcta atggcggacg
     3541 gggagtggat accactgacg ccgcagctaa cgcgataccc gctgcacaaa cactggcacg
     3601 ggaaactggc gcaatcgtcg tggtcactgg cgaggtggat tatgttaccg atggacatcg
     3661 tatcattggt attcacggtg gcgatccgtt aatgaccaaa gtggtaggaa ctggctgtgc
     3721 attatcggcg gttgtcgctg cctgctgtgc gttaccaggc gatacgctgg aaaatgtcgc
     3781 atctgcctgt cactggatga aacaagccgg agaacgcgca gtcgccagaa gcgaggggcc
     3841 aggcagtttt gttccacatt tccttgatgc gctctggcaa ttgacgcagg aggtgcaggc
     3901 atgaaacgaa ttaacgctct gacgattgcc ggtactgatc cgagtggtgg tgcggggatt
     3961 ctggccgatc ttaaaacctt ctcggcactt ggcgcttatg gttgctcagt tattactgca
     4021 ctggtggcgc aaaatacccg tggcgtacag tcggtgtatc gcattgagcc tgattttgtc
     4081 gccgcccagc tcgattcggt gttcagcgat gtgcgaatcg ataccactaa aatcggtatg
     4141 ttggcggaaa ccgatattgt tgaagcggtg gcagaacggt tgcaacgtta tcagatccaa
     4201 aacgtggtac tcgacaccgt tatgctggca aaaagcggcg acccgctgct ttcaccttcg
     4261 gcggttgcta cgctgcgcag tcgattattg ccacaggttt cattaataac gccaaacttg
     4321 cccgaagctg ccgccttgct cgacgcgcca cacgcgcgca ccgaacagga aatgctggaa
     4381 caagggcgat cgctgttggc gatgggctgt ggcgcagtgc taatgaaagg tggtcatctg
     4441 gatgatgagc aaagcccgga ctggctgttt acccgcgagg gtgaacaacg gtttaccgca
     4501 ccgcgcatta tgaccaaaaa cacccacggc actggttgta cactctctgc ggcgttggct
     4561 gcactacgcc cgcgccatac aaactgggct gacaccgtac aggaggcaaa aagctggctt
     4621 tcatcggcgt tagcccaggc cgacacgctg gaagttggtc acggtattgg tccggttcac
     4681 cacttccacg cctggtggtg agcaccgtac tatagctgct ttggttattg tcagcctttg
     4741 gcactgacat tcagggatga aaccaatgca gctaagaatt accagtcgaa aaaaactcac
     4801 cgctctatta tgcgctcttg ggctaatttc catcgttgca atttatccgc gtcagacggt
     4861 gaattttttc tattcgacag cagttcagat tacagactac atccacttct acggttatcg
     4921 cccggttaaa tctttcgcta ttcgcattcc tgccagttac accattcacg gattagatgt
     4981 ttcgcgctgg caggagcgta tcgactggca gcgggtggca aaaatgcgcg acaacgatat
     5041 ccgcttacag tttgctttta ttaaggctac ggaaggcgaa aagctggtgg acccctattt
     5101 ttcgcgtaac tggcaattaa gccgcgaaaa tggcctgctg cgcggggcgt atcattattt
     5161 ctccccgtcg gtatccgctt cagttcaggc gcgattattt ctgcaaacgg tggatttctc
     5221 acaaggcgat ttccctgccg tgctggacgt agaagaacgg ggaaaattat cggcaaaaga
     5281 attacgcaag cgggtaagtc agtggctaaa aatggtcgaa aaacgcacgg gaaaaaagcc
     5341 gattatttac tcaggagccg ttttttatca caccaatctg gcgggctatt tcaatgaata
     5401 tccgtggtgg gtggctcact attatcaacg tcgtccggac aatgacggca tggcctggcg
     5461 cttctggcag cattccgacc gtggacaggt agatggcatc aatggtccgg tggattttaa
     5521 tgtgtttaat ggcacggtgg atgagttgca ggcattcgtt gatgggatta aagaaacgcc
     5581 ttaaataaga agcagtgata tactcccacc agactccctg gcaggaaaaa gacaaatgga
     5641 acaagcgcat acccagctga ttgcccaact gaacgagcgt attttagcgg cggataacac
     5701 gccgctttat attaagtttg ccgaaacggt aaaaaatgcc gtgcgcagcg gggtgctgga
     5761 gcatggcaat attttgcccg gtgagcgtga tttaagtcag ttaaccggcg tttcgcgcat
     5821 tacggtgcgc aaggcgatgc aggcgctgga agaagagggc gtggtgacgc gttcgcgcgg
     5881 ttacggcacg cagatcaaca acatcttcga atactcgttg aaagaagcga gaggtttttc
     5941 tcagcaggtg gtattgcgcg ggaaaaagcc cgatacgcta tgggttaata agcgtgtcgt
     6001 gaaatgtccc gaagaagttg cgcagcagct ggcggtcgaa gcgggaagtg atgtcttttt
     6061 gcttaagcgt attcgctatg tcgatgaaga agcggtatcg attgaggaat cgtgggttcc
     6121 ggcgcattta attcatgatg ttgatgccat cggaatttct ctttatgact atttccgcag
     6181 ccagcacatt tacccacaac gtacgcgttc ccgcgttagc gcccggatgc cggatgccgg
     6241 atgccgagtt tcagtcacat attcagttag atagcaaaat accggtgctg gtgatcaagc
     6301 aagtggcgct tgaccaacag caacggccta ttgagtacag cattagccac tgtcgcagcg
     6361 atctatacgt ttttgtgtgc gaggagtagt tcctcgcgcg ttggcgcaca atcaccgccc
     6421 cgatgcccga caacccacga cgccactgca ttgcccagta atacggcatc cgccagtggc
     6481 agaccagagg ccagcccggc aagtacgcca cctgcatgac tgtcgcccgc cccaatggtg
     6541 tctacaactt gcgttggaaa tgcccgaatg cagccagaag cgtcgttgct gaaataccat
     6601 gcgccttctt tatcgagacg aacgatcaac ggcgcggcaa atttctcctg ccattgcttg
     6661 ccaagtgttg ttatctctgc agataaagca aaacgttcgg cggcaatctc agcctcttga
     6721 cgattgagtg acactaaagg tcgacaggcc atgatccgcg ccagtaatgc gtccgggata
     6781 tcgccaatac gtgggccaaa atcgataaac ggcgtcacgt cttgcagctt ttccagccat
     6841 tccactaaca attcgccgca gggcgaggcc agttgataac cggaaaaata gagcaggttg
     6901 ccaggcgcaa cggttaatcg tgccaaccat tggcgattcc attgattttc cacaccgctg
     6961 aatgacataa aagtgcgttc gccatccggc tcaaccagtg ccagacacca gccgttatcg
     7021 ctttcggcgt tatcgatcag gctgattaac ccctctttcg ccatccggtt gcgaataatc
     7081 tccgcccaca cgccctgacc gagcggcaag gcattacccg cttcgatgcc gaggcgcttt
     7141 aacgccacgg caatattcag tgcgcagccg ccaacgttaa cgctctgctg tttcagttcg
     7201 atatcgcacc cacgccaggg gagggcataa gcgtcggcga tcacgtcaat gaccgccgcg
     7261 ccgacgacca tcaccggctg acgcgtggtt aattcaggca gcagtgtgtg taatcgagcg
     7321 ccgctcatac cgcctcccgt tgttgacgat atttcgccag cgctgtggca tagcggttga
     7381 aatcaagctg atttaccgca tccagctccg cctttaacgc aggatcgaca gcgttaatgc
     7441 catgcaacgc gccgcaaatc gccgtcgcca tagcacctat ggtgtctgtg tcgccgccaa
     7501 gattggcgca caggacggcg cagcggtttg ggtcggtttg tgccagttca actagcgcaa
     7561 tggcgcacgg aacggactca atagtgctgg tgcctgcgcc aacgacctgg taaagctgtt
     7621 cgctggcgga ttcggtgccg tcggcattac gcacaatttt cagcgcaatc tccagacgtg
     7681 ccgccagtga ggcgctgaac gtggtggtgc gtttttgctg tgcatgtcgc gcaattgaag
     7741 gcaatgaatc aacaatcgct gaccagcgtt ctccgtcaat ggcacgagaa atcgcccatg
     7801 cgatgactac cgcgcctgca accgccagat cggatttatg ggtcgggctg gacgccagcg
     7861 ccacatcatc aataaaggaa tcaacatcac gcgctggaag caaacaacct aatggcgaga
     7921 cgcgcatcgc tgcgccgttg gttacgccgt tgttttccag ttcagcaacg ggtttaccgt
     7981 cgcgaatggc gttaagcgca atcttcgagg tcggacctag tacgtttttg ttaaaggcgt
     8041 cgaaacgcaa cgcccagtcg agaatattac gcccaatcag attcggatcg atcttgcctt
     8101 cacattccag taacgcatcc gccagacaca ggccatcgag gtatcgtagg tgaattcggc
     8161 gcggttaaaa taacaggccg cgttattctc ctttggtcca ggcagaaaac ggtcaatcca
     8221 gccaaagtgt gctttaacgc ggctgcgtgg ccaaagctcg gagggcatcc ccatcgcatc
     8281 ccctaacgcc tgcccataaa gagcaccgag aatacgttct gttttcattt aacttcccct
     8341 tgtgtcaacg caatatctcg atcatcgacc ttgatagccg taatttcgtt gtcggattcg
     8401 cggaaaaaaa tcatgaacag cacggcgata atggcaatca tcaccgcgcc gaaagtccac
     8461 atcccggacc agttgaaagt cagtccgttt accggttcct gataagcgaa catcttttcc
     8521 atcatcacac cgccaagacg atagccgagc aaactgccga agccctggca gcagagcgtg
     8581 atcagcccct gcgcagcggt acgcatatgc acgggggctt ttttatcgac atagatgtaa
     8641 gcggtaacgt agtaaaaatc gtaacttacg ccgtgaagca aaataccgag gaacagtaac
     8701 gcgtaggtga aattgacagg taatgtaagg tacgcgatga caataccaca taataatacc
     8761 ttttgaatac ctttgcggtg aaaacttgac cgcgctaaca ttttttctga tgaatcgagc
     8821 caacagaaaa cgctgaaaaa acatccaaaa gatggaaaaa ctcgcccgta acgaccagga
     8881 ttatgattgc ccgcaaaagg cgggccagga caatccatag ccgatatcca atcggaattt
     8941 acgggagcat agtaatgaca gatattgcgc agttgcttgg caaagacgcc gacaaccttt
     9001 tacagcaccg ttgtatgact attccttctg accagcttta tctccccgga catgactacg
     9061 tagaccgcgt gatgattgac aataatcgcc cgccagcggt gttacgtaat atgcagacgt
     9121 tgtacaacac cgggcgtctg gctggcacag gatatctttc tattctgccg gttgaccagg
     9181 gcgttgagca ctctgccgga gcttcatttg ctgctaaccc gctctacttt gacccgaaaa
     9241 acattgttga actggcgatc gaagcgggct gtaactgtgt ggcatcaact tacggcgtgt
     9301 tggtgtcggt atcgcggcgc tatgcgcatc gcattccatt tctcgtcaaa cttaatcaca
     9361 acgagacgct aagttacccg aatacctacg atcaaacgct gtatgccagc gtggagcagg
     9421 ccttcaacat gggcgcggtg gcggttggtg cgactatcta ttttggctcg gaagagtcac
     9481 gtcgccagat tgaagaaatt tctgcggcct ttgaacgcgc gcacgagctg ggcatggtgg
     9541 cagtgctgtg ggcctatttg cgaaactccg cctttaagaa agatggcgtt gattaccatg
     9601 tttccgccga cctgactggt caggcgaacc atctggcggc aaccataggt gcagatatcg
     9661 tcaaacaaaa aatggcggaa aataacggcg gctataaagc gattaattac ggttataccg
     9721 acgatcgcgt gtacagcaag ttaaccagcg aaaacccgat tgatctggtg cgttatcagt
     9781 tagctaactg ctatatgggc cgggctgggt tgataaactc cggcggtgct gcaggcggtg
     9841 aaactgacct cagcgatgca gtgcgtactg cggttatcaa caaacgcgca ggcggaatgg
     9901 ggctgattct tggacgtaag gcgttcaaga aatcgatggc tgacggcgtg aaactgatta
     9961 atgccgtgca ggacgtttat ctcgatagca aaattactat tgcctgatac gcctcatacg
    10021 aacagcacca ccccgtagga cggataaggc gtttacactg catccggcaa cctatgcctg
    10081 atgcgacgcg gaagcgtctt atcatgccta catagcactg ccacgtatgt ttacaccgca
    10141 tccggcataa aaacacgcgc actttgctac ggcttcccta tcgggaggcc gtttttttgc
    10201 ctttcactcc tcgaacaatt ttcatattgt cgtttttgtg atcgttatct cgacatttaa
    10261 aagcaaataa tttcattata ttttgaaatc gaaagcaaac gacaagatat gaaaatgtac
    10321 gtggtatcga caaagcagat gctgaacaac gcacagcgcg gcggttatgc ggttccggca
    10381 ttcaatattc acaatctcga aacgatgcaa gtggtggtgg aaaccgctgc caacctgcat
    10441 gcgccggtca tcatcgccgg aacgcctggc acatttactc atgctggtac agaaaatctg
    10501 ttggcgctgg tcagcgcgat ggcgaagcaa tatcaccatc cactggcaat tcatctcgac
    10561 catcacacga aatttgacga tatcgctcag aaggttcgtt ctggcgtgcg ctcagtcatg
    10621 attgacgcct cgcatttgcc ttttgcgcaa aatatttcac ggctcaaaga ggtggtggat
    10681 ttttgccatc gctttgatgt cagcgtcgag gcggagctgg ggcaacttgg cggccaggaa
    10741 gatgatgtgc aagtcaatga agccgatgcg ttgtacacca acctcgcaca ggcgcgtgaa
    10801 tttgccgagg caaccgggat tgattccctg gcggtcgcca tcggcacggc tcacggtatg
    10861 tatgccagcg caccggtgct tgatttttct cgactggaga acattcgcca gtgggtgaac
    10921 ttaccgctgg tgctgcatgg cgcgtcagga ttatcgacta aggatattca gcaaaccatc
    10981 aaactgggga tatgcaaaat caacgttgca acggagctga aaaatgcctt ctcgcagtcg
    11041 ttaaaaaatt acctgaccga gcaccccgaa gcgaccgatc cccgggatta tttgcagtcg
    11101 gctaaatccg caatgcgcga tgtggtgagc aaagtgattg ccgattgtgg ctgcgagggc
    11161 agggcataac gcacctgcca tttaacaagg aaaaaacatg aaaacgttaa ttgcccggca
    11221 taaagctggt gaacatatcg gcatatgttc agtctgttct gcccatccgt tggttatcga
    11281 agcggcgctg gcatttgatc gcaacagcac gcgcaaagtg ctgattgaag caacgtcaaa
    11341 ccaggtcaat caatttggcg gttataccgg aatgacaccg gcagactttc gcgaatttgt
    11401 ttttacgatt gccgataaag ttgggtttgc acgcgaacgc attattctcg gcggcgatca
    11461 tctggggcca aactgctggc agcaagaaaa tgcggatgcg gcgatggaaa aatccgtcga
    11521 gctggtaaaa gcatatgttc gtgctggctt cagtaaaatt catcttgatg cgtcaatgtc
    11581 ctgcgcgggg gatcccatac cgttagcacc agaaacggtt gcggaacgag ctgttgtgct
    11641 ttgctttgct gcggaaagtg tggcgacaga ttgccagcgt gagcaactga gctatgtcat
    11701 tggcactgaa gttccggttc cgggcggtga ggccagcgcc attcagtcag tacacatcac
    11761 ccatgttgaa gatgccgcca atactttacg tacgcatcaa aaggccttta ttgcccgtgg
    11821 gctgacagag gcgttaacac gtgtgattgc catcgtggtg cagccgggtg tggaatttga
    11881 tcacagcaat attatccatt atcagccgca ggaagcgcag gcgctggcgc aatggataga
    11941 aaacacccga atggtttatg aagcacattc taccgattac cagacccgga cggcttattg
    12001 ggaattagtc cgcgatcact ttgcaatatt gaaagtcggt cccgcattaa cctttgcttt
    12061 acgcgaggcg atatttgcac tggcacaaat tgagcaggaa cttatcgccc ccgaaaatcg
    12121 cagcggttgc ctggcggtaa ttgaagaagt gatgctcgac gaaccgcaat actggaaaaa
    12181 atattatcgc acgggtttta acgattcatt actggatatt cgttacagcc tgtcggatcg
    12241 tattcgttat tactggccgc atagtcggat taaaaatagc gttgaaacga tgatggtgaa
    12301 tctgcaaggc gtggacatcc cactgggcat gattagtcag tatcttccca aacaatttga
    12361 acgcattcag tccggggaat tatcagcaat gccgcatcag cttattatga ataaaattta
    12421 tgatgttttg cgcgcctatc gctacggctg tgcggaataa ggacggtata tgactaacct
    12481 gtttgttcgt agcggaattt cttttgtcga tcgtagcgaa gttttaacct atatcggtaa
    12541 tgagatgctc gcgaaaggtg tggttcatga tacctggcca caggcattaa ttgccagaga
    12601 agcagaattc cctaccggga taatgcttga gcagcacgcc attgcaatac cgcattgtga
    12661 ggcgattcat gctaagtcgt cagccattta tctgttaagg ccaacaaata aagttcattt
    12721 tcagcaagcg gatgatgata acgacgtggc ggtatcgttg gttattgcgt tgattgtgga
    12781 aaatccgcag cagcaattga aacttttacg ctgtttattt ggcaagttac aacagcccga
    12841 tatcgtcgag acactaatta ctcttcctga aactcagtta aaggaatact tcacaaagta
    12901 tgttttagat tcagacgaat aaatccctct gtaacaataa aaaggactat ttatgaaacg
    12961 caagattatt gtcgcttgcg gaggcgcggt tgcgacctct acgatggcgg cggaagaaat
    13021 taaagagttg tgtcagagtc ataatattcc tgttgaatta atccagtgtc gggttaatga
    13081 aatagaaacc tatatggatg gtgtgcattt gatatgtacc actgccaggg tggatcgtag
    13141 ttttggcgat attccgttag ttcacggcat gccttttgtt tctggtgtcg gtatcgaagc
    13201 attacaaaat aaaattctga ctatcttaca ggggtgacct atgttttcag aagtcatgcg
    13261 ttatattctc gacctcggcc ctacagtgat gctgccggtt gtcatcatta ttttttctaa
    13321 aatattaggc atgaaggcag gcgattgctt taaagcgggt ctgcatatcg ggattggctt
    13381 tgttggcatt ggccttgtga ttggcttaat gctggattcc attggtccgg cggcgaaagc
    13441 gatggcggaa aatttcgacc tgaatctgca tgtggtcgat gtcggctggc cgggctcttc
    13501 accaatgacc tgggcgtcgc aaattgcgct ggtggcgatt ccgattgcga tattggttaa
    13561 cgtggcgatg ttactgaccc gtatgacgcg ggtggtaaat gttgatatct ggaatatctg
    13621 gcatatgacc ttcaccggca gcgttgctgc acctggcaac cggttcatgg atgataggga
    13681 tggcgggtgt ggtaattcac gcggcgtttg tttataagct cggcgactgg tttgcccgcg
    13741 atacccgaaa tttctttgag ctggaaggta ttgctattcc gcacggtacg tcggcgtata
    13801 tggggccgat tgcggtgctg gtcgatgcta tcatcgagaa aatcccaggc gttaaccgaa
    13861 ttaaatttag cgccgacgat attcagcgca aatttggccc ttttggcgaa cctgtcaccg
    13921 tgggttttgt gatggggctg attatcggca tcctcgcggg ttacgatgtc aaaggtgtat
    13981 tgcagctggc ggtaaaaacg gcggcagtta tgctgctgat gccacggatg attaaaccca
    14041 tcatggatgg tttaacgccc atcgctaagc aggcccgtag tcgtttacag gcgaagttcg
    14101 gcggtcagga gttcctgatt ggcctggatc ccgcattact gctggggcat acggcggtgg
    14161 tatcggcaag cctgattttt attccgctca ccattttaat tgctgtttgt gttccgggta
    14221 atcaggtact gccgtttggc gatcttgcca ctatcggctt cttcgtggcg atggcggtcg
    14281 cggtgcatcg tggaaatctg ttccgcacct taatctcggg tgtcatcatt atgagcatca
    14341 ccctgtggat cgcgacgcaa actattggtt tgcacaccca actggcggct aatgctgggg
    14401 cgttaaaagc cgggggtatg gtggcttcaa tggatcaggg cggttctccc attacctggt
    14461 tactgattca ggttttctcc ccgcaaaata ttcccggttt cattattatc ggcgcaattt
    14521 atctgaccgg tattttcatg acctggcgta gagcgcgtgg ttttactaaa caagagaaag
    14581 ccgttctcgc agaataattt ttacctgagg gggaggttat cccccttaac aatcaggagt
    14641 ttttatgaaa tcagtagtga atgatactga tggtatcgtg cgcgttgcaa aaagcgtcat
    14701 tcctgaaatt aaacatcagg atgaggtgcg ggtaaaaatt gccagctcgg gcttatgtgg
    14761 ttccgattta cccaggatat ttaaaaatgg tgcacattat tatccaataa cgttaggcca
    14821 tgaatttagc ggctatattg atgcggtggg atccggtgtt gatgatttac accctggcga
    14881 tgcggttgcc tgtgtgccgt tattaccctg ttttacttgt ccagagtgtt tgaaagggtt
    14941 ttattcccag tgcgcaaaat atgattttat tggctcgcgg cgtgatggtg gatttgctga
    15001 atatattgtc gttaagcgaa aaaatgtctt tgctctaccc acggatatgc ctattgagga
    15061 tggggctttt attgagccga ttaccgttgg cctgcatgct tttcatttag cgcaaggttg
    15121 tgagaataaa aacgttatta ttattggtgc cggaaccatt ggcctgctgg ctattcagtg
    15181 cgctgtcgcg ctgggagcaa agagtgtgac ggcgatcgac attagctcag aaaaactggc
    15241 actggcaaaa tctttcggtg cgatgcaaac atttaacagt agcgaaatga gcgcgccgca
    15301 aatgcagagc gttttacgcg aactgcgctt taatcagctt atcctcgaga cggctggcgt
    15361 accgcaaact gtcgaactgg cggtagagat tgccggtcct catgcccaac tggcgctggt
    15421 gggcacgttg catcaggatc tgcatttaac atcggcaacg tttggcaaaa tattgcgtaa
    15481 agagctgacg gttatcggca gttggatgaa ctattccagc ccttggccgg ggcaggagtg
    15541 ggaaacggcg agccggttgc tgacagaacg taagttaagc ctggatccat taatcgctca
    15601 ccgtggaagc tttgaaagct tcacccaggc ggtgcgtgac atcgctcgta atgccatgcc
    15661 gggcaaagtg ttgctcattc catgaaaccg cgggccagcg tgatgctggc ccggtattgt
    15721 gcaaaacaga tcattcacca atggtccccc ttcgtttaca ctagccacaa ttgaatatgg
    15781 gtaaatgacg atgaattcat tcgagcgaag gaataagatc atccaattag tgaatgaaca
    15841 gggaaccgtg cttgttcagg atctggcggg agtatttgct gcctcggaag cgacaatccg
    15901 tgccgatttg cgctttctcg aacaaaaagg cgtggttacg cgctttcatg gcggtgcggc
    15961 gaaaataatg tccggtaata gtgaaaccga gacccaggaa gtcgggttta aagagcgatt
    16021 tcagctcgcc agcgcgccaa aaaacagaat agcgcaggcg gcagtcaaaa tgatccatga
    16081 agggatgacc gttattctcg acagcggaag taccacaatg cttatcgccg aaggattaat
    16141 gaccgctaaa aatatcacgg tgattaccaa cagtctcccg gcagcgtttg ccctttccga
    16201 aaataaagac attactctgg tcgtctgtgg tggcacggtc cgccataaaa cgcgctcgat
    16261 gcatggttcc attgccgagc gttcattgca ggatattaat gccgatttaa tgtttgtcgg
    16321 tgctgatggt attgatgcgg ttaatggtat tacgaccttt aatgaaggtt attcgattag
    16381 cggggcgatg gtcaccgccg caaataaagt gattgccgtt ctcgattcat cgaaatttaa
    16441 tcgtcgcggt tttaatcagg tactgccaat cgaaaaaatt gacattattg ttaccgatga
    16501 tgctgtgtca gaggtggata aattggcatt gcagaagaca cgggtaaaat taattacggt
    16561 gtagtattgt tctgtatgcc ggataaggtg tttacaccgc atccggcatt acggattctc
    16621 cctggttcaa gagagaatag tttgattaac gcaataatgg acaatctggt ggtaatcgac
    16681 aacgcaacgc cgccggaagt atttcaatat ggaagttttg cccactcaac ggttcgccat
    16741 caagattaaa agtgatttcg tgtggggctt gtatatcaaa ccacgacgaa gcgccttcga
    16801 taatattcgg gttatcttcg tcagatttta aggttgatac gagagccgga agaatttcat
    16861 cgccggtaaa aatgcgcagt tgcagcaagc catcgttaat taacgcgttc gggcacaatt
    16921 gttgaccgcc accggcctga cgcccgttac caataccaat gaccagggcg tcaccttgcc
    16981 agtgaaagtt ttcaccgcgg atttcacaac ggtccggttg cagagtatcc atgcgcatta
    17041 agccatgaat gatgtaagag acgccaccca gcgcggcttt taatttttcc ggcgtttctg
    17101 tggtaatacg cgtcccaaat ccgcctgtcg ccatattaat aaaacaggtt tgtttgttga
    17161 cctgcgccat atctatcgca atggcattac cggcaattgc cagtttcagc gccttatcca
    17221 gtgcctcagg aatccctaca ctggtggcaa aatcattggc ggttcctaat ggcaaaattc
    17281 ccagcgcggg tatgtcatcc ccctcacact gaatcaacgc cgtagaaact tcattaatgg
    17341 tgccatcgcc accaccggca atcaccgttg cgacgcccaa cttccgggcc tcctctacaa
    17401 atcgtgcggc atcacctttc tcccaggtga cccgcacatg gatcgtcatt ccttcctcac
    17461 gcaacagcat aattgcttcg cgcaagggta gattgtcagt acttttgcca ttaagaatca
    17521 gtaagctggc gggaaattct gccatgatcg tatgtgcctt tatgattggt ctgtagatag
    17581 tgtagagcag aaaacaaaag gtggagtcag aacaggatga aagtcggagg ataaaaagtc
    17641 agttcaagta tggaggattc agtggttacc accaggagct tgcaatgggg acagcgaata
    17701 ctaacggctg ccaccgataa atttcaaaaa agagcatata cctaatattc aactaaacag
    17761 tggcatcttc aatataatat attaaagccc ccatggagtt accctgaagg gcctcaatgt
    17821 ccgtaattcc tacttatgta ggaaatgttg tacagaacat ttattataat cctattcaat
    17881 tataataatc atgccattat tatatttaaa cactagagag tgtcgttggt atttaatggg
    17941 ggaaggtgag atgaaaaaga tagctgctat atcattaatt agtatttttc ttatatctgg
    18001 ttgtgctgtg cataatgatg aaacaagtat cggtaaattt ggtcttgcat ataaaagtaa
    18061 tattcagcgt aaactcgata accaatacta caccgaagcc gaagcttctt tagccagggg
    18121 cagaatatct ggtgcagaaa atatagtaaa aaatgatgca gcccatttct gtgttactca
    18181 gggcaaaaaa atgcagatag ttgacctgaa gacagaaggt gcaggattac atggcgtcgc
    18241 tcgtctgaca ttcaaatgtg gagagtgaga atattttttg gtaagcgtca aacatgcgcg
    18301 ttctggctgt gcgtagccgg aacctgggcg agcacgatac cgttacgtga aaggcattgt
    18361 gctatgaagg gggattctat cgatgtggtc aatggaagac ggttatcagg gatcaatcct
    18421 tctttacagc ttatgtacaa aaaataagcc cgtgtaaggg agattacaca ggctaaggag
    18481 gtggttcctg gtacagctag cattttatgg attatgtttt tcagcgaaac ggatgataac
    18541 cttaataaat gcagctgtat gtgatcggtt tctaagaatt ttccatccgg gaaaaataat
    18601 cgaaattaat cacttaccgt gggggttacg cgtggtttcc ccggagaaat tacgcattag
    18661 cagcgcgtaa tttagctcaa gatcctgcgg gaccgggagc cacacagtat aaccatcgcc
    18721 tggtgcgacc ggcataactt cgcctttggc gttttccatg tgctcaaggg taaagttaat
    18781 gttgccttgc ggcgtcatca gctcaagact gtcgccaacg gagaatttat ttttcaccgc
    18841 taccgctgcg aggtccccct tgcgctcacc ggtaaactca ccaacaaact gctggcggtc
    18901 agaaactgaa taaccgtatt cgtagttctg ataatcgtcg tgagtatgac gacgcaggaa
    18961 accttcggta tagccacgat gcgccagacc ttccagagtt tccagcaggc tggtatcgaa
    19021 cggttttccc gcagcggcgt catcgatagc tttgcggtaa acctgtgcgg tgcgtgcgca
    19081 atagtagaaa gatttggtac gaccttcgat tttcagcgaa tgcacgccca ttttggtcag
    19141 gcgttctaca tgggcgatgg cgcgcagatc tttcgagttc atgatgtaag tgccgtgctc
    19201 atcttcaaac gcggtcatat actcgcccgg acgctgggct tcttcgatca taaacacttt
    19261 gtcggttggc gcgccgatac ccagcgtcgg ctcaacattt tgcaccggaa tcggctcgta
    19321 cttgtgtacg atgttgccaa cgtcatcttc tttcccttcc tggacgttgt actcccagcg
    19381 gcaggcgttg gtgcaggtgc cctggttcgg gtcgcgcttg ttgatatagc cagagagcag
    19441 gcagcgaccg gagtaggcca tgcacagtgc gccgtgaacg aagatctcga tctccatatc
    19501 cggcacctga ttatggatct cttcaatctc ttccagcgac agctcgcgag agaggatcac
    19561 gcgggtcagt cccatttgct gccagaattt caccgtcgcc cagttcacgg cgttagcctg
    19621 caccgagagg tggatcggca tttctgggaa gtgctcacgc accagcataa tcagccctgg
    19681 atcggacata atcagcgcat ccggccccat ttccaccacc ggtttcaggt cacggataaa
    19741 ggttttcagc ttggcgttgt gtggtgcaat gttgaccacg acataaaact ttttccccag
    19801 cgcgtgggct tcattgatgc cgagctgaag attttcgtgg ttgaattcgt tgttgcgcac
    19861 acgtagggag taacgcggct ggcccgcata aacagcatct gcgccataag cgaaagcgta
    19921 acgcatattt ttcagcgttc ccgccgggga aaggagttcc ggtttaaaca taattctctc
    19981 gttctgatga caggtcagat ccgcttcacc tgatgaagcg gttaggggag gcccccactt
    20041 taagggcggg cattgtagcg ctgtaggggg gatctggtaa agcgccggac gccaacaacc
    20101 cacgcccggc atcgtaatta cgtattgtct ttcaccgtct ggcttgtgcc attggcttta
    20161 acggaggcaa ttccggtttc acgagattgc gcggtggcgt acatctggct ggagccgata
    20221 atttgatgat tagcggcttt cagattgaaa tagaatttgc cgtttttttc tcatagcgct
    20281 cttcttgcgg gctgttgcta cgcaccgacg cgatgccctt ttccgcagag gcttttgagg
    20341 tatacagctc gctggtgagg atagtctcac cattgcccgc ttttagcaca aaccggaact
    20401 gattatcact gctcttactg agttcaaacc aaccagccat aatgactcct tgattgaaat
    20461 tgcttatcga aaacagcatc taaaggtgct gtttactgat taaagtatga gcgattttct
    20521 gatgcctgcc agggcggaga gataatgtca gtttgctgac catgcgtgga ataatgttgc
    20581 cggatcgggt ctgaacgcca tctccggcta acaaagtaat gtcgctaaaa ctaaacgatg
    20641 cggcaggcgt cggcttccca gcggtaacca acgccgtaaa cggcgcggat aaatgactgt
    20701 tcggcgtcga gagattccag cttgcggcgc agatttttaa tgtggctgtc aatggtgcgg
    20761 tcggttacta cgcggtagtc gtcataaaga tgattgagca attgctcgcg ggagaacact
    20821 tttcctgatt cgtgagagag cgttttaagc agacgaaatt ccgcaggcgt caggtcaagc
    20881 attttaccgc gccatgaagc ctgaaaacga ccttcgtcga taatcaacgg gctttcggca
    20941 tcctgttgct gaagttcgcg ttgcggttta caacggcgca aaatggtttt gacacgcgct
    21001 accacttccc gtgggctgta cggcttgcag atgtagtcat cggcgccaat ctccagcccc
    21061 agcaggcggt cgatctcttc gatttttgcc gtcaccatca cgatcggaac atcggaaaaa
    21121 cgacgaattt cccggcataa tgtcaggcca tcggtgccag ggagcatcag atccaacagg
    21181 atcagatccg gtggtgtctg gcgcacatac gccagtacct gatcgccgtg gctgataagc
    21241 gtcggcgcat agctcgcagc acgcagataa tcaatgagca actgccccag cttcggttca
    21301 tcttccacga tcaaaatacg cggtgtgttt tcgtcgattg gtaactcggt catacttctc
    21361 tctgtaaatc ccgttccagc ggtaactcta ctgtaatgct taccccgcca aaaggcgaat
    21421 gggcggcaat aatgcggcca ttatgggctt caacaatgtt caggcaaatc gccagcccca
    21481 gcccggaacc gccgctggcg cggttgcggg aaccttcggt gcgataaaaa cgttcaaaca
    21541 atttttgtag ctgatcgtca ctgacacctg gcgcactgtc ggcaaaggtc aggcgcaccg
    21601 ttttgtcgtg ctgctcggca gagattttca ggctgccgcc gctgtcagtg tagcgcaggc
    21661 tgttttccag taagttattg aataactgca ttaaacggtc gcgatcgcca aatacggtga
    21721 tactgtctgg cagggaaaat tgcagtttca ggccacggct ggcgaatcgt tcgcgaaatg
    21781 cgccgcccgc cacttccagc agtgggatca aatctaccgg tgctttttga taggcgagag
    21841 cgccttcatc agacatcgac aactgatgaa gatcgtcaac cagtttggtc agtgtaccga
    21901 cctccgcctg taaagacgcc accgtctccg gcgtgaattt gcgcacgcca tcctgaatgg
    21961 cttccagttc accgcgcagc accgctaatg gcgtacgcag ttcgtgagaa atatcggcca
    22021 taaaatcgcg ccgcatttgc tggtttttct ccagcgtgct ggcgagctgg ttgaagtctt
    22081 gcgccagttt gcccagttca tcttcactgg tgggcgttac gcgggtagtg aaatcgcccg
    22141 ccgccagttt gtgcgtgcca tcgataagtc gttttaccgg tgccagcaaa ccgcgcgcca
    22201 gcagaaaggt ggcgagcgcc gcgagtaacg ttgccagggc gacgatcagc cagctggttt
    22261 gccgctgttg tttatcgaaa ttgatatcag tattgcgggt cagccgctca acaggggagg
    22321 caatcaccgc gcccacttcc gcaccgttga ccagaatggg tcgtcgtgta ccgtcaggtg
    22381 gaatcggcgc tcgcggacca accagtactt tgttgttttg atcgaccacc cagaactggg
    22441 tacgccagcc gtgcggcggc atacccgggc cgggtttatc ttccgaatta tcgtgttcaa
    22501 atgaacgcag gatctgaaag acaaagcgat cgttgttgcg caggaagcgc cagttaccat
    22561 gctgcgcata ctgctcgcca agcgcatcac ttaacagctg taatcgctgt tcattgccgt
    22621 gtttgatgta atcaataaag ccacgctcaa aactgatacg caccgcccag tgcatactga
    22681 tcagcaagac aatgcaggtg gcgaaaatcg ccagaaacag tttgccggta ataccgggtc
    22741 gccagaactt cattgcgcgc tcctttttcg ccgcgaaata gctacatttt gatgcgtatc
    22801 gttcggcact ctggcaaaga tgaacgccgg aagggcgatg ataaacgcca tgctaagcca
    22861 ggtgtacata aagacggttt gtgtggtgcc gctgtcgacg ctgacatgct gcgaaccaaa
    22921 aagccccagc aacagcccgg cgatggtgac gccgatactc atcgacaatg gcataatcat
    22981 cgacagcagg ctgtttccgc tgctcgccag attgtccggg agatctttca gcgtcagggt
    23041 gttcatggag gagaaacggg tcgagttgac catcccttgt aaaaacagga cgaacggcaa
    23101 aacgtagtac cagcccaaca gagcggtagt cataaacaac agggtgacca gcgacagacc
    23161 cagcgtggtc gctaccagta cccgacgata accaaagcga ttcaccacct gtaccacaat
    23221 tcgcttcatt cccatgctgc caagcaccat cgggatcatc atcagtccgg catgaaacgg
    23281 cgagaaaccg aggccaattt gcaggaaaac cggtgtcata aagggcaaca tgccactgcc
    23341 aatacgtccg gcaaagctcc ccgccaggcc cagcgaaaag gtacgagtgc ggaacagttt
    23401 cagactgaac agggcgcggt tgttatttct ggcgtgcagc agataaagca ccagtgccac
    23461 cacgccaact gcgaccaggc cagcaatcgc cagcggcgat aaacctgtac ctttactgcc
    23521 gtccagcgcc agggttaata ccgccatgcc aaccgccagc aataaaaagc cggaaagatc
    23581 aaagcgccgc gtctgcatgg tgtagttcgg cattaacatc aatgtggcga tcgcaccgat
    23641 aatccccacc ggaatgttga tcaaaaagat ccagtgccac gacgcgtatt ctaccagcag
    23701 accgccgagc gccggaccga gcagcggacc gacctgaccg ggtaacgtga caaaggtcat
    23761 cgccgccata tattgctcgc gcggtacgat tttcatcacc gtcaacctgc cgaccggcac
    23821 catcatcgca ccgccaacgc cctgtaacgc gcgtgccagc aacagttcgt tcagcgtgcc
    23881 ggaaagcgcg caaaacagtg aaccgagagt aaacagcacg atggcggtaa agaaaatatt
    23941 gcgcacgccg actttgtccg ccagccagcc gctggcgggc agcatcaccg ccacggtcag
    24001 cacataagag acaatgacca tgtgcatatg caacggactt tccccgaggc tttgcgccat
    24061 tgagggaagg gcggtgttta cgatggtggt gtccagcgac tgcataaaga agccgaaagc
    24121 cacaatccat aattgccagc gggtgctgtc gggaagatct gtcatttact cggttaccgt
    24181 ttgtttaggt ttacgcgaaa aacgcagccg cagacggtcg aaaaagagat acaccaccgg
    24241 cgtggtatac agcgtcagga gctggctcat caccagaccg ccgacaatgg tgatcctcag
    24301 gggttgccgc agctccgagc cgtcaccgcc cgacaatacc agcggcagcg cgccaaacag
    24361 cgccgccagg gtggtcatca taatcgggcg aaaacgcagc agacaggcct ggaaaatagc
    24421 ttcctgcggc gtcaggttac cgtgccgttg ggcttcaagc gcaaaatcga ccatcataat
    24481 ggcgtttttc ttcacgatgc cgattaatag catgatccct atcagggcga ttaggctgaa
    24541 cggggcattg aacagctcca gcgccaacag cgctccaacg cccgccgagg gcagggtgga
    24601 gagaatcgtc agcggatgta cgtaactctc gtaaaggata cccagcacga tatacaccgt
    24661 ggcgatggcg gcgataatca ggatcacctg cgagttcatc gtctcctgga aaacctgcgc
    24721 cgtgccggca aaactgccgc gcaccgtcga aggcacacca agctgggtca ttgcgcgatc
    24781 gatcgccgca ctggcgtccg agagcgattt tccggtcggc aggttgaacg agatggtcga
    24841 ggccgccgat aatccctgat gattcaccga tagtggggca ttcgccggtt gccatttagc
    24901 gaaatacgat agcgggatcg ctttgccttc gttattgata acgaacattt tttccagcgc
    24961 actgatgtcc tgggtatagc gcggatccac ttccatcacc actttatact ggttcatcgg
    25021 ctggtaaatg gtcgagattt gccgctgacc gaaggcgtta tttaacagac tgttggcggc
    25081 ctgtacgtcg attcccagcc gtgccatggt gtcgcggtcg taaaccagat tcatctccgc
    25141 gccgttatcc tgctgatcgg agttcacgtc cgccagttcc ggcaacgtcg ccagtttttt
    25201 gcggattttc ggctcccatt cgcgcagtgc cgccaggtcg tcggataaca acgtgtactg
    25261 gtagctggcg ttcgactgac gcccaccaac gcgaatatct tgtaccgcca tcaggaacag
    25321 attcgccccc ggttctttcg ccagcttcac gcgcagacgg tcgataattt gctgcgccgt
    25381 ttcgctgcgt tcgtcgcgtg gcttgagggt gataaacatc atcccactgt taactcgtga
    25441 accgccggta aagccggtga cattatccac tgccggatcg tcacggataa ttttcatgaa
    25501 atcctgcaat ttgccgcgca tcgcctgaaa cgaaatactc tgatccgcct gtatcccacc
    25561 catcagtacg ccggtgtcct gctccgggaa gaaggttttc gggatcgaga tattcagcgc
    25621 aatggtgcca agcagcacca cgcccaccag acgggtatga ttgagcaccc atttcagcga
    25681 cttgccgtag ccttgttgca gggctaccaa catgcggcca aaaccacgca gtcgcttttg
    25741 ctcacgcggc ttgctggctt ttagcatcca gccgcacatc attggcgtta gcgtcaggga
    25801 aaccagcaac gaaataccaa tggcgacaga aagcgtcacg gcaaactcgc gtaacagtcg
    25861 gcccggcaat ccgcccatca acagcagcgg caggaacacc gccaccagtg acagactcat
    25921 cgacagcacc gtaaagccga cttcgcgagt accttgcagc gcggcctgta gtggtttcat
    25981 ccccgcttcc agatgacggg caatgttttc cagcaccaca atggcgtcat ccaccacgaa
    26041 accagtagca atggtgagcg ccatcagcga aagattattg agactgaatc cgcacaggta
    26101 catcgccgca aacgtaccaa tcagcgaaac cggcaccgca acggcgggaa taatagtggc
    26161 gcgacccgag cgcaggaata aaaagaccac cagaatcacc agcgccaccg agataatcag
    26221 cgtttgctcg acttcttcca gcgaggcgcg aatggtgggg gagcgatcct gggcaatttg
    26281 cagatcaatc gccgccggaa tggtctcctg caactctggt aattttgccc ggatgctgtc
    26341 taccgtctgg ataatattgg cttccggcag tttgcggatc atcagtaaaa tagccggttt
    26401 ggcgttggtc atcccggcgt tgcgcacatc ctgcactgag tcagtcaccg tcgccacatc
    26461 gcccagacga accgcgccgc cgttgttgta gtgaataatc agcggctgat attcagcggc
    26521 ggttttcagt tcatcgttgg tctggatctg ccagcggtga gtgccatctt ccagcgcccc
    26581 ctgcggttta cgcacgttgg cattgctgat ggcagtgcgt acgtcgtcca gcgacacgcc
    26641 ctgattaaac agcgcctgcg ggttcagccc gacgcgcacg gcgggcagcg agctgccacc
    26701 gacatcgaca tcaccgacac cgtcgatttg cgaaatcgtc ggagccagct gcgtcgaggc
    26761 gaaatcgtac agttcaccct gcgaataagt atcggatgtc agcgtgagga tcataattgg
    26821 cgcatccgac gggttcgctt tgcgataggt cgggcggctg ggcatcccgc tgggcagcaa
    26881 actttgtgca gcgttgatcg ccgcctgcac atcacgcgct gcgccgttga tatcccggtc
    26941 aaaatcaaac tgcaaaataa tgcgcgtgct gccgagcgaa ctgctggagg tcatttcact
    27001 aaccccggca atgcgcccaa gtgagcgctc cagcggcgtg gcaacggaag acgccattgt
    27061 ctccggcgac gcaccgggca gcgaggcgct gaccataatc accggaaaat cgacctgcgg
    27121 cagcggggcg accggcagca tacggaaacc cagtatgccg cacagggtaa tggcgaccga
    27181 cagtaaaatc gtcgccaccg ggcggtaaat gaagagggca aaaaacttca cttacgcctc
    27241 ctcttcatgg cgagcaaagc gacttttggt ccacaatacc aagcggtcga acagcagata
    27301 aatcaccggc gtggtgaaca gcgtcagcac ctggctgaca atcagaccgc cgaccatgcc
    27361 gatacctaac ggacgacgca gttccgcgcc gaccccggta ctcaacatca gcggcagcgc
    27421 gccaagcaga gccgccaggg tggtcatcag gatcggacga aaacgcaaca gacaagcctg
    27481 gtagattgca tcgcgcggcg acatgccttg ctcgcgctca gcagccagcg cgaagtcgat
    27541 catcatgatg gcgttcttct tcacaatacc gatcagcaaa ataatgccga taatcgcaat
    27601 cacatccagt tcgctaccgg caatcattaa tgccagcagc gcgccgaccc ctgcggtggg
    27661 tagcgtcgag agaatggtga tcgggtgaat aaagctctcg tacagaatgc cgagcacgat
    27721 atacatcgcc accaccgccg cgacaatcag ccagacagtg ctgcccagcg ccgactggaa
    27781 ggcgagggtg ctgccctgga attgcgtggt gatatccacc ggcagattca gcgtcttttc
    27841 ggtgtccata atcgcctgca ccgcatcgcc cagcgaatag ttatccggca cgttaaagga
    27901 gatggttgtt accgggaact gatccagatg gttgatggaa agcggcgcaa aacgctgctc
    27961 aattttggca attgagctta acggcaccac gccaccgtcg ctgctggtca ggcgaatggt
    28021 atccagcgcc gcgaggcctg gggtattttc ggtattgtgc tccagcacca cgcgatactg
    28081 gttagcctga gtataaatag tggaaatcag tcgctgacca aacgcgttgt acagggcgtt
    28141 atcgacgtcc gccatgctga tccccagacg gctggcgctg tcgcgatcaa cattgacata
    28201 cgccaccagc cctttgtcct gccagtcgct ggagacatca gaaagctgtg gcagttgctg
    28261 gagtttttcc atcaactgtg gcacccaggt actgagcgca tccagtgacg tggcctgcaa
    28321 ggtaaactgg tactgggtgc ggctgacctg agtatcgatg gtcaggtcct gcgttggttg
    28381 caggaagaga tcgacgcccg gcactttatc taccgccgtt tgcagacggg cgatgacttt
    28441 ttgcacccga tcatcacgtt catccaacga tttgaggttg atttgtaaac gtgcactatt
    28501 cagcgacggg ttagtgccat caacgccaac aaatgaggtc aggctttgca ctgccggatc
    28561 ctgcaaaatc acgtccgcga cctggcgttg tcgctgggcc atattggcaa aggagctgga
    28621 ttgcggtgcc tgcaaagtgc cctgaataat gccgttgtcc tgtaccggga agaaaccttt
    28681 cggaatgaac acccacagca gcacgctaag cagcagcgtg ctgagtgcca cgcttaaggt
    28741 cagccacgga tgattcagca ctttcgccag tccacgacca taggcggcga ttatcctgtc
    28801 gaacattttt tccgaggcac gggagaagcg gttctgttta cgcaacgact cctggctgag
    28861 catccgcgcg cacatcatcg gtgtcagggt cagcgacacc accgctgaga tcaaaatcgc
    28921 taccgccagg gtaatagcaa attcgcggaa cagtcgcccg acgatatcgc ccataaacag
    28981 cagtgggatc aacaccgcaa tcagtgagaa ggtcagcgag ataatggtaa agccgatttc
    29041 acctgcgccc ttgagcgccg ccgccaacgg tttttcgcct ttttcgatat agcgggaaat
    29101 gttttcgatc accacgatgg cgtcatcgac cacgaatccg gtggcgatag ttaacgccat
    29161 cagtgtcagg ttattgattg aaaaatcgag aaacaccata accgcgaaag tgccgattaa
    29221 cgacagcggt acagcaacac cgggaatgat ggtcgccgga atattgcgca aaaacaggta
    29281 gataatcatg actaccagcg cgatagccat catcaattca aactgagtat catcgacgga
    29341 tgcgcggata ttggtggtgc gatcggaaag cactgtcacc ttcaccgatt tcggcagact
    29401 ctcagtgagc tgtggcagca tctgccgaat gctgtcggcg gtggagataa tgttagcacc
    29461 gggctggcgc tgaacattca tcacaatggc ctgttctttg ttcgcccacg cgccgagcca
    29521 gctgttttct gcaccttgct ctacagttgc tacatcgccc agacgaattg gcgcgccgtt
    29581 ctggtaggcg atgattagct ggcgatactc ttcggcggat tgcatctggt cgttcgcgga
    29641 aagcgtgacc gcacgggaag ggccgtcgag gctacctttt gccgagttaa cgttagcgcc
    29701 ggtaatggcg gtgcgcacgg tttcgctggt caggccgagg gcggcaatcg cctgagcgtt
    29761 aagtttgacg cgaacagccg gacgctgacc gccggaaagc gtcaccaggc cgacgccgga
    29821 aatctgcgag attttctgcg cgacgcgggt ttccaccata tcttccactt gcgtcatcgg
    29881 catggcggtt gaggtgacgg cgagcgtcat gatcggcgga tctgccgggt tcactttgct
    29941 gtaaaccggc gggttaggca gatcgctcgg caacaagttg gtcgcagcgt taatcgcggc
    30001 ctgcacttcc tgctcggcga cattgagcgg taatgttagc tggaactgca aagtgataac
    30061 tgacgcaccg ccggaacttt gcgacgacat ctgtttcagg ccagacattt gcccgaactg
    30121 gcgttcgagc ggcgcggtaa cggcagaggt catgacatcc gggctggcac ttgggtagag
    30181 cgtgaccacc tgaatggtcg gatagtccac ttccggcagc gccgaaacgg gcagggcgcg
    30241 ataaccgata atcccggcga gtaagatcgc caccatcagc agcgtggtgg cgacaggacg
    30301 cataataaac aggcgcgacg ggccgcctgt gctgctcggg ggtaacacct gcatcaggag
    30361 cgtgctcctt ttttcgcgta ttcgcggctg gtggctttct cttccggagt ggtggcgctc
    30421 tgggcttcca ccacttccac tttctgactg tcctgaatgc ccggcgtcac cagatgtttg
    30481 ctgaccttgt tttcgctatt cagcacccag acaaaatggc cttcattgcc catttgcagg
    30541 gcggctgttg ggatcactac ggcgttttgt tcggtgtcga ctaacatgcg cgcgttaaca
    30601 aactgattgg gaaacagcgc atcatcctga ttattaaagc gtgctttcac tttaatcgta
    30661 ccggttgtgg catcgatttg gttatcgagg cttaacagcg tgccttcact taatttcttc
    30721 gaattggtgc gatcccaggc ttctaccatc agcggttttc cggctttttg cgcctgcact
    30781 acagtagcga tatcgctttc cggcagggta aagactaaat cgataggatg cgtctgggtg
    30841 atcaccacaa ttccggtggt atcaccactg gagatttggt taccaacatc aacttgcttg
    30901 agaccaacgc gaccatcgac tggtgcggtg atgcggctcc agtcgagttg caactgcgcg
    30961 ctggcgacgc ttgcttcatc agccttaatg gtgccttcgg tttcactgac cagcgcctgt
    31021 tgggcatcca gctcctgacg ggagacgaga ttggtttttg ccagttgctg ataacgcgcc
    31081 agatcacggc gggcgttagc aagcgtggct ttatctttta ccagttggcc ctgggtttgt
    31141 gctaatgcaa ctttgaactg gctggggtca atttctgcca gtaaatcgcc tgctttgacc
    31201 tgctggcctt cctggaaatg taacgccatc agttgaccgt ccacgcggct gcgcaccgta
    31261 acagtattag cggcggtaat ggtgccaagc ccggt
//
LOCUS       DADMEQ010000038        29995 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.038, whole genome shotgun sequence.
ACCESSION   DADMEQ010000038 DADMEQ010000000
VERSION     DADMEQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29995
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.038"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            286..669
                     /gene="secE"
                     /locus_tag="JNZ05_001758"
     CDS             286..669
                     /gene="secE"
                     /locus_tag="JNZ05_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAY8565019.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            671..1216
                     /gene="nusG"
                     /locus_tag="JNZ05_001759"
     CDS             671..1216
                     /gene="nusG"
                     /locus_tag="JNZ05_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAY8565020.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1375..1803
                     /gene="rplK"
                     /locus_tag="JNZ05_001760"
     CDS             1375..1803
                     /gene="rplK"
                     /locus_tag="JNZ05_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAY8565021.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1807..2511
                     /gene="rplA"
                     /locus_tag="JNZ05_001761"
     CDS             1807..2511
                     /gene="rplA"
                     /locus_tag="JNZ05_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAY8565022.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2924..3421
                     /gene="rplJ"
                     /locus_tag="JNZ05_001762"
     CDS             2924..3421
                     /gene="rplJ"
                     /locus_tag="JNZ05_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAY8565023.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3488..3853
                     /gene="rplL"
                     /locus_tag="JNZ05_001763"
     CDS             3488..3853
                     /gene="rplL"
                     /locus_tag="JNZ05_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAY8565024.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4173..8201
                     /gene="rpoB"
                     /locus_tag="JNZ05_001764"
     CDS             4173..8201
                     /gene="rpoB"
                     /locus_tag="JNZ05_001764"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAY8565025.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EGGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8278..12501
                     /gene="rpoC"
                     /locus_tag="JNZ05_001765"
     CDS             8278..12501
                     /gene="rpoC"
                     /locus_tag="JNZ05_001765"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAY8565026.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12743..13876)
                     /gene="thiH"
                     /locus_tag="JNZ05_001766"
     CDS             complement(12743..13876)
                     /gene="thiH"
                     /locus_tag="JNZ05_001766"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HAY8565027.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANNCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13873..14718)
                     /gene="thiG"
                     /locus_tag="JNZ05_001767"
     CDS             complement(13873..14718)
                     /gene="thiG"
                     /locus_tag="JNZ05_001767"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAY8565028.1"
                     /translation="MSSGRNISCRMATRSCFFRLLQGVEMLRIVDKTFDSHLFTGTGK
                     FASSQLMVEAIRASGSQLVTLAMKRVDLRQHNDAILEPLIAAGVALLPNTSGAKTAEE
                     AIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAETLVQQGFVVLPYCGADPV
                     LCKRLEEVGCAAVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVVDAGIGVPSHAAQA
                     LEMGADAVLVNTAIAVADDPVNMAKAFRLAVEAGLLARQSGPGSRSYFAHATSPLTGF
                     LEASA"
     gene            complement(14645..14845)
                     /gene="thiS"
                     /locus_tag="JNZ05_001768"
     CDS             complement(14645..14845)
                     /gene="thiS"
                     /locus_tag="JNZ05_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HAY8565029.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14829..15584)
                     /gene="thiF"
                     /locus_tag="JNZ05_001769"
     CDS             complement(14829..15584)
                     /gene="thiF"
                     /locus_tag="JNZ05_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HAY8565030.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15577..16212)
                     /gene="thiE"
                     /locus_tag="JNZ05_001770"
     CDS             complement(15577..16212)
                     /gene="thiE"
                     /locus_tag="JNZ05_001770"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAY8565031.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKKMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16212..18107)
                     /gene="thiC"
                     /locus_tag="JNZ05_001771"
     CDS             complement(16212..18107)
                     /gene="thiC"
                     /locus_tag="JNZ05_001771"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HAY8565032.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18188..18288)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18341..18817)
                     /gene="rsd"
                     /locus_tag="JNZ05_001772"
     CDS             complement(18341..18817)
                     /gene="rsd"
                     /locus_tag="JNZ05_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HAY8565033.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18912..19685
                     /gene="nudC"
                     /locus_tag="JNZ05_001773"
     CDS             18912..19685
                     /gene="nudC"
                     /locus_tag="JNZ05_001773"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HAY8565034.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19725..20789
                     /gene="hemE"
                     /locus_tag="JNZ05_001774"
     CDS             19725..20789
                     /gene="hemE"
                     /locus_tag="JNZ05_001774"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HAY8565035.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20946..21617
                     /gene="nfi"
                     /locus_tag="JNZ05_001775"
     CDS             20946..21617
                     /gene="nfi"
                     /locus_tag="JNZ05_001775"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HAY8565036.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21660..22250
                     /locus_tag="JNZ05_001776"
     CDS             21660..22250
                     /locus_tag="JNZ05_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HAY8565037.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22437..22709
                     /gene="hupA"
                     /locus_tag="JNZ05_001777"
     CDS             22437..22709
                     /gene="hupA"
                     /locus_tag="JNZ05_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HAY8565038.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22722..23417
                     /locus_tag="JNZ05_001778"
     CDS             22722..23417
                     /locus_tag="JNZ05_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HAY8565039.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23419..23844)
                     /gene="zraP"
                     /locus_tag="JNZ05_001779"
     CDS             complement(23419..23844)
                     /gene="zraP"
                     /locus_tag="JNZ05_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HAY8565040.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24082..25458
                     /gene="zraS"
                     /locus_tag="JNZ05_001780"
     CDS             24082..25458
                     /gene="zraS"
                     /locus_tag="JNZ05_001780"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HAY8565041.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25455..26780
                     /gene="zraR"
                     /locus_tag="JNZ05_001781"
     CDS             25455..26780
                     /gene="zraR"
                     /locus_tag="JNZ05_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HAY8565042.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDFVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26777..28066)
                     /gene="purD"
                     /locus_tag="JNZ05_001782"
     CDS             complement(26777..28066)
                     /gene="purD"
                     /locus_tag="JNZ05_001782"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAY8565043.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28078..29667)
                     /gene="purH"
                     /locus_tag="JNZ05_001783"
     CDS             complement(28078..29667)
                     /gene="purH"
                     /locus_tag="JNZ05_001783"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAY8565044.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7017 a         7820 c         8253 g         6905 t
ORIGIN      
        1 tccgtgaagg cggccgtacc gttggcgcgg gcgttgtagc aaaagttctg agctaattgc
       61 cgataacatt tgacgcaatg cgcactaaaa gggcatcatt tgatgccctt tttgcacgct
      121 ttcgtaccag aacctggctc atcagtgatt ttctttgtca taatcattgc tgagacaggc
      181 tctgttgagg gcgtataatc cgaaaagcta atacgcgttt cgatttggtt tgcctcgcga
      241 tcgcggggtg aaaatgtttg tagaaaactt ctgacaggtt ggtttatgag tgcgaatacc
      301 gaagctcaag gaagcgggcg cggcctggaa gcgatgaagt gggtcgttgt ggtggcattg
      361 ctcctggcgg cgattgtcgg caactacctt tatcgcgaca ttatgctgcc gctgcgtgcg
      421 ctggccgtag taattctgat tgctgcagcg ggtggtgtcg cgctgttaac gacaaaaggt
      481 aaagctaccg ttgcttttgc ccgtgaagcg cgtaccgaag tccgtaaggt catttggccg
      541 actcgccagg aaacattgca caccacgctg attgtggctg cggttaccgc agtaatgtca
      601 ctgatcctgt ggggactgga tggtattctg gttcgcctgg tatcctttat cactggcctg
      661 aggttctgag atgtctgaag ctcctaaaaa gcgctggtac gtcgttcagg cgttttccgg
      721 ttttgaaggc cgcgtagcaa cgtcgctgcg tgagcatatc aaattacaca acatggaaga
      781 tttgtttggt gaagtcatgg taccaaccga agaagtggtt gaaatccgtg gcggtcagcg
      841 tcgcaaaagc gaacgtaaat tcttccctgg ctacgtcctc gttcagatgg tgatgaacga
      901 cgcgagctgg cacttggtgc gcagcgtacc gcgtgtgatg ggcttcatcg gcggtacttc
      961 cgatcgtcct gcgccaatca gcgataaaga agtcgatgcg attatgaacc gcctgcagca
     1021 ggttggtgat aagccgcgtc cgaaaacgct gtttgaaccg ggtgaaatgg tccgtgttaa
     1081 tgatggtccg ttcgctgact tcaacggtgt tgttgaagaa gtggattacg agaaatctcg
     1141 tctgaaagtg tctgtttcta tcttcggtcg tgcgaccccg gtagagctgg acttcagcca
     1201 ggttgaaaaa gcctaaccca gcgatcaaaa aagcggcgat ttaatcgttg cacaaggcgt
     1261 gagattggaa tacaatttcg cgccttttgt ttttatgggc cttgcccgta aaacgatttt
     1321 ttatatcacg gggagcctct cagaggcgtt attacccaac ttgaggaatt tataatggct
     1381 aagaaagtac aagcctatgt caagctgcag gttgcagctg gtatggctaa cccgagtccg
     1441 ccagtaggtc cggctctggg tcagcagggc gtaaacatca tggaattctg caaagcgttc
     1501 aacgcaaaaa ctgattccat cgaaaaaggt ctgccgattc cggtagtaat caccgtttac
     1561 gctgaccgtt ctttcacttt cgttaccaag accccgccgg cagcagttct gctgaaaaaa
     1621 gcggctggta tcaagtctgg ttccggtaag ccgaacaaag acaaagtggg taaaatttcc
     1681 cgcgctcagc tgcaggaaat cgcgcagacc aaagctgccg acatgactgg tgccgacatt
     1741 gaagcgatga ctcgctccat cgaaggtact gcacgttcca tgggcctggt agtggaggac
     1801 taagaaatgg ctaaactgac caagcgcatg cgtgttatcc gcgagaaagt tgatgcaacc
     1861 aaacagtacg acatcaacga agctatcgca ctgctgaaag agctggcgac tgctaaattc
     1921 gtagaaagcg tggacgtagc tgttaacctc ggcatcgacg ctcgtaaatc tgaccagaac
     1981 gtacgtggtg caactgtact gccgcacggt actggccgtt ccgttcgcgt agccgtattt
     2041 acccaaggtg caaacgctga agctgctaaa gctgcaggcg cagaactggt aggtatggaa
     2101 gatctggctg accagatcaa gaaaggcgaa atgaactttg acgttgttat tgcttctccg
     2161 gatgcaatgc gcgttgttgg ccagctgggc caggttctgg gtccgcgcgg cctgatgcca
     2221 aacccgaaag tgggtactgt aacaccgaac gttgctgaag cggttaaaaa cgctaaagct
     2281 ggccaggttc gttaccgtaa cgacaaaaac ggcatcatcc acaccaccat cggtaaagtg
     2341 gactttgacg ctgacaaact gaaagaaaac ctggaagctc tgctggttgc gctgaaaaaa
     2401 gcaaaaccga ctcaggcgaa aggcgtgtac atcaagaaag ttagcatctc caccaccatg
     2461 ggtgcaggtg ttgcagttga ccaggctggc ctgagcgctt ctgtaaacta atgcctttac
     2521 gtgggcggtg attttgtcta caatcttacc cccacgtata atgcttaatg cagacgtata
     2581 tccgagatat tcgggttgtg gcaaggcggc aactgagtga gtcgccagga gcatagctaa
     2641 ctatgtgact ggtgcgaatg aaggaagcca acgccgccac aagttgaata gcgacggata
     2701 gaaaagattt gttcgttgga gcctggccta tccaggcctc cgtcgaagac cgcaggagtt
     2761 tcgcaagaaa cttaatcccc tgcgtagacg gtgacagaac gctaagatta ttcttttata
     2821 ttctggcttg tttctgctca ccgtaattaa gacgctctct ccgtttggag gagtgaagtg
     2881 agttccagag attttctctg gcaaacatcc aggagcaaag ctaatggctt taaatcttca
     2941 agacaaacaa gcgattgttg ctgaagtcag cgaagtagcc aaaggcgcgc tgtctgcagt
     3001 agttgcggat tcccgtggcg taactgtaga taaaatgact gaactgcgta aagcaggtcg
     3061 cgaagctggc gtatacatgc gtgttgttcg taacaccctg ctgcgccgtg ctgttgaagg
     3121 tactccgttc gagtgcctga aagacgcgtt tgttggtccg accctgattg catactctat
     3181 ggaacacccg ggcgctgctg ctcgtctgtt caaagagttc gcgaaagcga atgcaaaatt
     3241 tgaggtcaaa gccgctgcct ttgaaggtga gctgatcccg gcgtctcaga tcgaccgcct
     3301 ggcaactctg ccgacctacg aagaagcaat tgcacgcctg atggcaacca tgaaagaagc
     3361 ttcggctggc aaactggttc gtactctggc tgctgtacgc gatgcgaaag aagctgctta
     3421 atcgcagtta tctttttaac gcattcgctt acgtataaac ttattctgat attcaggaac
     3481 aatttaaatg tctatcacta aagatcaaat cattgaagca gttgcagcta tgtctgtaat
     3541 ggacgttgta gaactgatct ctgcaatgga agaaaaattc ggtgtttccg ctgctgctgc
     3601 tgtagctgta gctgctggcc cggttgaagc tgctgaagaa aaaactgaat tcgacgtaat
     3661 tctgaaagct gctggcgcta acaaagttgc tgttatcaaa gcagtacgtg gcgcaactgg
     3721 cctgggtctg aaagaagcta aagacctggt agaatctgca ccggctgctc tgaaagaagg
     3781 cgtgagcaaa gacgacgcag aagcactgaa aaaagctctg gaagaagctg gcgctgaagt
     3841 tgaagttaaa taagccaacc cttccggttg cagcctgaga aatcaggctg atggctggtg
     3901 actttttagt caccagcctt tttgcgctgt aaggcgccag tagcgtttca cactgtttga
     3961 ctactgctgt gcctttcaat gcttgtttct atcgacgact taatatactg cgacaggacg
     4021 tccgttctgt gtaaatcgca atgaaatggt ttaagcgtga tagcaacagg cattgcggaa
     4081 agtgttccat tttccggtca acaaaatagt gttgcacaaa ctgtccgctc aatggacaga
     4141 tgggtcgact tgtcagcgag ctgaggaacc ctatggttta ctcctatacc gagaaaaaac
     4201 gtattcgtaa ggattttggt aaacgtccac aagttctgga tgtaccttat ctcctttcta
     4261 tccagcttga ctcgtttcag aaatttatcg agcaagatcc tgaagggcag tatggtctgg
     4321 aagctgcttt ccgttctgta ttcccgattc agagctacag cggtaattcc gagctgcaat
     4381 acgtcagcta ccgccttggc gaaccggtgt ttgacgtcca ggaatgtcaa atccgtggcg
     4441 tgacctattc cgcaccgctg cgcgttaaac tgcgtctggt gatctatgag cgcgaagcgc
     4501 cggaaggcac cgtaaaagac attaaagaac aagaagtcta catgggcgaa attccgctca
     4561 tgacagacaa cggtaccttt gttatcaacg gtactgagcg tgttatcgtt tcccagctgc
     4621 accgtagtcc gggcgtcttc tttgactccg acaaaggtaa aacccactct tcgggtaaag
     4681 tgctgtataa cgcgcgcatc atcccttacc gtggttcctg gctggacttc gaattcgatc
     4741 cgaaggacaa cctgttcgta cgtatcgacc gtcgccgtaa actgcctgcg accatcattc
     4801 tgcgcgccct gaactacacc acagagcaga tcctcgacct gttctttgaa aaagttatct
     4861 ttgaaatccg tgataacaag ctgcagatgg aactggtgcc ggaacgcctg cgtggtgaaa
     4921 ccgcatcttt tgacatcgaa gctaacggta aagtgtacgt agaaaaaggc cgccgtatca
     4981 ctgcgcgcca cattcgccag ctggaaaaag acgacgtcaa actgatcgaa gtcccggttg
     5041 agtacatcgc aggtaaagtg gttgctaaag actatattga tgagtctacc ggcgagctga
     5101 tctgcgcagc gaacatggag ctgagcctgg atctgctggc taagctgagc cagtctggtc
     5161 acaagcgtat cgaaacgctg ttcaccaacg atctggatca cggcccatat atctctgaaa
     5221 ccttacgtgt cgacccaact aacgaccgtc tgagcgcact ggtagaaatc taccgcatga
     5281 tgcgccctgg cgagccgccg actcgtgaag cagctgaaag cctgttcgag aacctgttct
     5341 tctccgaaga ccgttatgac ttgtctgcgg ttggtcgtat gaagttcaac cgttctctgc
     5401 tgcgcgaaga aatcgaaggt tccggtattc tgagcaaaga cgacatcatt gatgttatga
     5461 aaaagctcat cgatatccgt aacggtaaag gcgaagtcga tgatatcgac cacctcggca
     5521 accgtcgtat ccgttccgtt ggcgaaatgg cggaaaacca gttccgcgtt ggcctggtac
     5581 gtgtagagcg tgcggtgaaa gagcgtctgt ctctgggcga tctggatacc ctgatgcctc
     5641 aggatatgat caacgccaag ccgatttccg cagcagtgaa agagttcttc ggttccagcc
     5701 agctgtctca gtttatggac cagaacaacc cgctgtctga gattacgcac aaacgtcgta
     5761 tctccgcact cggcccaggc ggtctgaccc gtgaacgtgc aggcttcgaa gttcgagacg
     5821 tacacccgac tcactacggt cgcgtatgtc caatcgaaac ccctgaaggt ccgaacatcg
     5881 gtctgatcaa ctctctgtcc gtgtacgcac agactaacga atacggcttc cttgagactc
     5941 cgtatcgtaa agtgaccgac ggtgttgtaa ctgacgaaat tcactacctg tctgctatcg
     6001 aagaaggcaa ctacgttatc gcccaggcga actccaacct ggatgaagga ggccacttcg
     6061 tagaagacct ggtaacttgc cgtagcaaag gcgaatccag cttgttcagc cgcgaccagg
     6121 ttgactacat ggatgtatcc acccagcagg tggtatccgt cggtgcgtcc ctgatcccgt
     6181 tcctggaaca cgatgacgcc aaccgtgcat tgatgggtgc gaacatgcaa cgtcaggccg
     6241 ttccgactct gcgcgctgat aagccgctgg ttggtactgg tatggaacgt gctgttgccg
     6301 ttgactccgg tgtaactgcg gtagctaaac gtggtggtgt cgttcagtac gtggatgctt
     6361 cccgtatcgt tatcaaagtt aacgaagacg agatgtatcc gggtgaagca ggtatcgaca
     6421 tctacaacct gaccaaatac acccgttcta accagaacac ctgtatcaac cagatgccgt
     6481 gtgtgtctct gggtgaaccg gttgaacgtg gcgacgtgct ggcagacggt ccgtccaccg
     6541 acctcggtga actggcgctt ggtcagaaca tgcgcgtagc gttcatgccg tggaatggtt
     6601 acaacttcga agactccatc ctcgtatccg agcgtgttgt tcaggaagac cgtttcacca
     6661 ccatccacat tcaggaactg gcgtgtgtgt cccgtgacac caagctgggt ccggaagaga
     6721 tcaccgctga catcccgaac gtgggtgaag ctgcgctctc caaactggat gaatccggta
     6781 tcgtttacat tggtgcggaa gtgaccggtg gcgacattct ggttggtaag gtaacgccga
     6841 aaggtgaaac tcagctgacc ccagaagaaa aactgctgcg tgcgatcttc ggtgagaaag
     6901 cctctgacgt taaagactct tctctgcgcg taccaaacgg tgtatccggt acggttatcg
     6961 acgttcaggt ctttactcgc gatggcgtag aaaaagacaa acgtgcgctg gaaatcgaag
     7021 aaatgcagct caaacaggcg aagaaagacc tgtctgaaga actgcagatc ctcgaagcgg
     7081 gtctgttcag ccgtatccgt gctgtgctgg tagccggtgg cgttgaagct gagaagctcg
     7141 acaaactgcc gcgcgatcgc tggctggagc tgggcctgac agacgaagag aaacaaaatc
     7201 agctggaaca gctggctgag cagtatgacg aactgaaaca cgagttcgag aagaaactcg
     7261 aagcgaaacg ccgaaaaatc acccagggcg acgatctggc accgggcgtg ctgaagattg
     7321 ttaaggtata tctggcggtt aaacgccgta tccagcctgg tgacaagatg gcaggtcgtc
     7381 acggtaacaa gggtgtaatt tctaagatca acccgatcga agatatgcct tacgatgaaa
     7441 acggtacgcc ggtagacatc gtactgaacc cgctgggcgt accgtctcgt atgaacatcg
     7501 gtcagatcct cgaaacccac ctgggtatgg ctgcgaaagg tatcggcgac aagatcaacg
     7561 ccatgctgaa acagcagcaa gaagtcgcga aactgcgcga attcatccag cgtgcgtacg
     7621 atctgggcgc tgacgttcgt cagaaagttg acctgagtac cttcagcgat gaagaagtta
     7681 tgcgtctggc tgaaaacctg cgcaaaggta tgccaatcgc aacgccggtg ttcgacggtg
     7741 cgaaagaagc agaaattaaa gagctgctga aacttggcga cctgccgact tccggtcaga
     7801 tccgcctgta cgatggtcgc actggtgaac agttcgagcg tccggtaacc gttggttaca
     7861 tgtacatgct gaaactgaac cacctggtcg acgacaagat gcacgcgcgt tccaccggtt
     7921 cttacagcct ggttactcag cagccgctgg gtggtaaggc acagttcggt ggtcagcgtt
     7981 tcggggagat ggaagtgtgg gcgctggaag catacggcgc agcatacacc ctgcaggaaa
     8041 tgctcactgt taagtctgat gacgtgaacg gtcgtaccaa gatgtataaa aacatcgtgg
     8101 acggcaacca tcagatggag ccgggcatgc cagaatcctt caacgtattg ttgaaagaga
     8161 ttcgttcgct gggtatcaac atcgaactgg aagacgagta attctcgctc aaacaggtca
     8221 ctgctgtcgg ggtaaaaccc ggcagcggat tgtgctaact ccgacgggag caaatccgtg
     8281 aaagatttat taaagtttct gaaagcgcag actaaaaccg aagagtttga tgcgatcaaa
     8341 attgctctgg cttcgccaga catgatccgt tcatggtctt tcggtgaagt taaaaagccg
     8401 gaaaccatca actaccgtac gttcaaacca gaacgtgacg gccttttctg cgcccgtatc
     8461 tttgggccgg taaaagatta cgagtgcctg tgcggtaagt acaagcgcct gaaacaccgt
     8521 ggcgtcatct gtgagaagtg cggcgttgaa gtgacccaga ctaaagtacg ccgtgagcgt
     8581 atgggccaca tcgaactggc ttccccgact gcgcacatct ggttcctgaa atcgctgccg
     8641 tcccgtatcg gtctgctgct cgatatgccg ctgcgcgata tcgaacgcgt actgtacttt
     8701 gaatcctatg tggttatcga aggcggtatg accaacctgg aacgtcagca gatcctgact
     8761 gaagagcagt atctggacgc gctggaagag ttcggtgacg aattcgacgc gaagatgggt
     8821 gcggaagcaa tccaggctct gctgaagagc atggatctgg agcaagagtg cgaacagctg
     8881 cgtgaagagc tgaacgaaac caactccgaa actaagcgta aaaagctgac caagcgtatc
     8941 aaactgctgg aagcgttcgt tcagtctggt aacaaaccag agtggatgat cctgaccgtt
     9001 ctgccggtac tgccgccaga tctgcgtccg ctggttccgc tggatggtgg tcgtttcgcg
     9061 acttctgacc tgaacgatct gtatcgtcgc gtcattaacc gtaacaaccg tctgaaacgt
     9121 ctgctggatc tggctgcgcc ggacatcatc gtacgtaacg aaaaacgtat gctgcaggaa
     9181 gcggtagacg ccctgctgga taacggtcgt cgcggtcgtg cgatcaccgg ttctaacaag
     9241 cgtcctctga aatctttggc cgacatgatc aaaggtaaac agggtcgttt ccgtcagaac
     9301 ctgctcggta agcgtgttga ctactccggt cgttctgtaa tcaccgtagg tccatacctg
     9361 cgtctgcatc agtgcggtct gccgaagaaa atggcgctgg agctgttcaa accgttcatc
     9421 tacggcaagc tggaactgcg tggtcttgct accaccatta aagctgcgaa gaaaatggtt
     9481 gagcgcgaag aagctgtcgt ttgggatatc ctggacgaag ttatccgcga acacccggta
     9541 ctgctgaacc gtgcaccgac tctgcaccgt ctgggtatcc aggcatttga accggtactg
     9601 atcgaaggta aagctatcca gctgcacccg ctggtttgtg cggcatataa cgccgacttc
     9661 gatggtgacc agatggctgt tcacgtaccg ctgacgctgg aagcccagct ggaagcgcgt
     9721 gcgctgatga tgtctaccaa caacatcctg tccccggcga acggcgaacc aatcatcgtt
     9781 ccgtctcagg acgttgtact gggtctgtac tacatgaccc gtgactgtgt taacgccaaa
     9841 ggcgaaggca tggtgctgac tggcccgaaa gaagcagaac gtctgtatcg ctctggtctg
     9901 gcttctctgc atgcgcgcgt taaagtgcgt atcaccgagt atgaaaaaga tgctaacggt
     9961 gaattagtag cgaaaaccag cctgaaagac acgactgttg gccgtgccat tctgtggatg
    10021 attgtaccga aaggtctgcc ttactccatc gtcaaccagg cgctgggtaa aaaagcaatc
    10081 tccaaaatgc tgaacacctg ctaccgcatt ctcggtctga aaccgaccgt tatttttgcg
    10141 gaccagatca tgtacaccgg cttcgcctat gcagcgcgtt ctggtgcatc tgttggcatc
    10201 gatgacatgg tcatcccgga gaagaaacac gaaatcatct ccgaggcaga agcagaagtt
    10261 gctgaaattc aggagcagtt ccagtctggt ctggtaactg cgggcgaacg ctacaacaaa
    10321 gttatcgata tctgggctgc ggcgaacgat cgtgtatcca aagcgatgat ggataacctg
    10381 caaactgaaa ccgttattaa ccgtgacggt caggaagaga agcaggtttc cttcaacagc
    10441 atctacatga tggccgactc cggtgcgcgt ggttctgcgg cacagattcg tcagcttgct
    10501 ggtatgcgtg gtctgatggc aaagccggat ggctccatca tcgaaacgcc aatcaccgcg
    10561 aacttccgtg aaggtctgaa cgtactccag tacttcatct ccacccacgg tgctcgtaaa
    10621 ggtctggcgg ataccgcact gaaaactgcg aactccggtt acctgactcg tcgtctggtt
    10681 gacgtggcgc aggacctggt ggttaccgaa gacgattgtg gtacccatga aggtatcatg
    10741 atgactccgg ttatcgaggg tggtgacgtt aaagagccgc tgcgcgatcg cgtactgggt
    10801 cgtgtaactg ctgaagacgt tctgaagccg ggtactgctg atatcctcgt tccgcgcaac
    10861 acgctgctgc acgaacagtg gtgtgacctg ctggaagaga actctgtcga cgcggtaaaa
    10921 gtacgttctg ttgtatcttg tgacaccgac tttggtgtat gtgcgcactg ctacggtcgt
    10981 gacctggcgc gtggccacat catcaacaaa ggtgaagcaa tcggtgttat cgcggcacag
    11041 tccatcggtg aaccgggtac acagctgacc atgcgtacgt tccacatcgg tggtgcggca
    11101 tctcgtgcgg ctgctgaatc cagcatccag gtgaaaaaca aaggtagcat caagctcagc
    11161 aacgtgaagt cggttgtgaa ctccagcggt aaactggtta tcacttcccg taacaccgaa
    11221 ctgaaactga tcgacgaatt cggtcgtacc aaagaaagct acaaagtgcc ttacggtgcg
    11281 gtactggcga aaggcgatgg cgaacaggtt gctggcggcg aaaccgttgc aaactgggac
    11341 ccgcacacca tgccggttat caccgaagta agcggttttg tacgctttac tgacatgatc
    11401 gacggccaga ccattactcg tcagaccgac gaactgaccg gtctgtcttc gctggtggtt
    11461 ctggattccg cagaacgtac cgcaggtggt aaagatctgc gtccggcact gaaaatcgtt
    11521 gatgctcagg gtaacgacgt tctgatccca ggtaccgata tgcctgcgca gtacttcctg
    11581 ccgggtaaag cgattgttca gctggaagat ggcgtacaga tcagctctgg tgacaccttg
    11641 gcgcgtattc cgcaggaatc cggcggtacc aaggacatca ccggtggtct gccgcgcgtt
    11701 gcggacctgt tcgaagctcg tcgtccgaaa gaaccggcaa tcctggctga aatcagcggt
    11761 atcgtttcct tcggtaaaga aaccaaaggt aaacgtcgtc tggttatcac cccggtagac
    11821 ggtagcgatc cgtacgaaga gatgattccg aaatggcgtc agctcaacgt gttcgaaggt
    11881 gaacgtgtag aacgtggtga cgtaatttcc gacggtccgg aagcgccgca cgacattctg
    11941 cgtctgcgtg gtgttcatgc tgtgactcgt tacatcgtta acgaagtaca ggacgtatac
    12001 cgtctgcagg gcgtaaagat taacgataaa cacatcgaag ttatcgttcg tcagatgctg
    12061 cgtaaagcta ccatcgttaa cgcgggcagc tccgacttcc tggaaggcga acaggttgaa
    12121 tactctcgcg tcaagatcgc aaaccgcgaa ctggaagcga acggcaaagt gggtgcaact
    12181 tactcccgcg atctgctggg tatcaccaaa gcgtctctgg caaccgagtc cttcatctcc
    12241 gcggcatcgt tccaggagac cactcgcgtg ctgaccgaag cagccgttgc gggcaaacgc
    12301 gacgaactgc gcggcctgaa agagaacgtt atcgtgggtc gtctgatccc ggcaggtacc
    12361 ggttacgcgt accaccagga tcgtatgcgt cgccgtgctg cgggtgaagc tccggctgca
    12421 ccgcaggtga ctgcagaaga cgcatctgcc agcctggcag aactgctgaa cgcaggtctg
    12481 ggcggttctg ataacgagta atcgttaatc cgcaaataac gtaaaaaccc gcttcggcgg
    12541 gtttttttat gggtgttatt cagagggcaa attcttcacg gaaaatatta agaaaggcag
    12601 ggcgaaacga ggaagaagct tttcgccctg gacaacattc ctgctgacgg cactaccata
    12661 aaaaaacaca acgctttcaa caaccttgcg tttttcatct tccgtagcgg ctcggaataa
    12721 atttacgttc ttacattccg tctcatagtc tttgcgaggc gcgtcccaga tagctgtccc
    12781 agtctttcca taccggctgc aaaccctgag cggttaacgc ggcagcaacc gcttccggtc
    12841 tgcgatcgtc gtgcggtgag aactgttcca actcggggtg attatcggca tagccacctg
    12901 gctgcgtttt cgagaaggcg ctgacgttat tgatcgccag cggaataacg cgatcgcgaa
    12961 accacggtga ttcccgcgtg gagagtgaca gttcaatctc cggtgcaagc agtcggaagg
    13021 cgcagatggt ttgcactaac tggcgttcat ccataatcga cgcaggctca atgccgccag
    13081 tacacgggcg caggcgcgga aaggagacag agtaacggct ttgccagtaa tgctgttgca
    13141 gccatagcaa atgttcggca accatatagc agtcaacgcg ccagttgtcg gaaaggccaa
    13201 ttagcgcgcc gaggcctatc ttatcaatcc ccgcacgccc cagccgatcc ggcgtttcca
    13261 gccgccagaa gaagtcctgt tttttgcctt tcaaatgatg gcgggtataa gtcgcctcgt
    13321 gatatgtctc ctgataaacc atcaccccat ccagaccaag ttgctttaac tcggcgtatt
    13381 ccgtctccgc cagcggttgc acttccattt gtagtgaaga gaactgttca cgcagggcgg
    13441 ggagatgacg acgaaagtaa tccatcccca ctttcgcctg atgttcacca gtgactaata
    13501 gcagatgttc aaagcccatc tcccgtatag cggcgctttc cctggcaata tccgcttcat
    13561 ccagcgtttt gcgcttgatg cgattactca tggaaaatcc gcagtacgtg cagttgttag
    13621 cgcaaagatt ggaaagataa agcgggacgt agaaactaac tgtgttgcca aatcgctgac
    13681 gggtcagacg ctgcgcctgt tgggccagtt gttccagata gccactggcg gcaggcgata
    13741 acagcgccat catgtcgtcg cgggtgagtt gcgaggcatt tagcgcccgc tctacgtcag
    13801 cagccgtttt gccgttgata cgcaggcgga tgtcgtccca gtccagttgt cgccagcgat
    13861 tgctgaaggt tttcatgccg atgcctccag aaatccggtc agcgggctgg tggcatgagc
    13921 aaaataactg cggctgcccg gtccggactg acgtgccagc aggcctgctt ctaccgccag
    13981 acgaaatgcc ttcgccatgt tgacgggatc gtccgcgacg gcaatcgccg tattcactaa
    14041 caccgcgtcg gcccccattt ccagcgcctg cgcggcatgg ctgggaacgc cgatgccagc
    14101 atcgacaacc accggaactg tggcctgctg gataataatc tccagcatgg cacgggtttc
    14161 cagtccctga ttcgagccaa tcggcgcgcc gagcggcatc accgctgcac agccgacttc
    14221 ttccagacgt ttacacaata ccggatcggc cccgcagtaa ggcagcacga caaatccctg
    14281 ttgtaccagc gtttcggcgg ctttcagggt ttcgatggga tcgggcaaca gccagcgggc
    14341 gtcagggtga atctccaatt ttagccagtt tgtgcctaac gcttcacgtg ccagatgggc
    14401 ggcgaaaatg gcttcttccg ctgttttcgc cccggaagta ttcggcagca gggccacacc
    14461 cgccgcgata agcggttcga ggatagcgtc gttgtgctgg cgcaagtcga cacgtttcat
    14521 cgccagtgtc accagctggc tgccggaagc gcggatcgcc tccaccatca gttgtgaaga
    14581 agcgaatttc cctgtgccgg taaacagatg tgaatcaaac gttttgtcca caatacgtaa
    14641 catctcaacc ccctgcaata acctgaaaaa gcaggatctg gtcgccatcc tgcacgatat
    14701 gttgcgtcca ctgctcacgc gggacgattt gctgattaat cgccagagcc gcgcccgctt
    14761 gtcgttggtc gagttgctcc agtagttcgt gaacagtttg cccggcggcg cactgcatcg
    14821 gttgatcgtt aaacaggatc tgcattgctt cctccgcata ccgggcaacc actggcgcgg
    14881 cgcaacgcca ggctgcgcca ctggctcgac ttaccgtcga acagtcggag ttctcccgca
    14941 ggcgtctcta taccgcttaa taacttaatg gcttccagtg cctgcaaagt gcccatcacc
    15001 ccgaccaccg ggccaaccac gcccgccgtg cggcagttgc gttctggctg ctggttatct
    15061 ggccacaggc agcggtaaca cccctgctcc cagggcggtg tcagtaccat caactgaccg
    15121 ccaaatccga ccgcgctggc ggtgataagc ggcgtgttga gtgccacgca ggcggcatta
    15181 atctcctggc gagtcgccat attgtcggta cagtcgagca ccacatcggc ttgtgcaacc
    15241 gcatctttca acgcctcacc cgttaaccgt tgttgtaatg ctgtcagttg aatgtcggga
    15301 ttcaactgtg tcagtcgctg ttggctgacc tgcgatttcg ggctgttgat atcttcagtg
    15361 gtaaagagga tttgtcgttg cagattgctt aaatgcacat cgtcgtcatc tgccagtacc
    15421 agcgtcccga cgccagcccc tgccaggtat agcgcagcag gtgtacccag cccgccaaga
    15481 ccaataatca gcacctggct gtcgagcagt ttttgctgcc cgtcaagagc gatatcgtcg
    15541 agcagaattt ggcggctata acgcataaag tcacggtcat tcatcgccaa ctcctgcaat
    15601 ttccagcaac tgtgccgttg ccaaacgcca gtctgcggct tgagtaatgg cgctgacgac
    15661 ggcgatactg ccgacacccg ttgctatcac cgcaggcgcg cgtgccagac tgataccgcc
    15721 aatcgccacg gtgggataat ccgccaggcg ctcaacatgc cgtgccaact gttccagccc
    15781 ctgcggtgca gaaggcatct ttttggtttg cgtcgggaac acatgtccca gcgcgatata
    15841 agaggggcgt gctgccagcg cgacgtcgat ttccatatcg tcatgtgtcg aaacgcccag
    15901 ccgcaggcct gccgcgcgga tggcattgag atcggtggct tgcaaatctt cctgccccaa
    15961 atggacgcca tacgcctgat gcttgatcgc gagccgccag taatcgttga taaacaatcg
    16021 cgcgttatag cggcggccca gcgcaattgc cgccacgaca tcggcttcca cctcttcatc
    16081 gcgccgatct ttgatgcgta gctggagagt acgtacgcct gcatccaaca gacgttcgat
    16141 ccactgtacg ctgtccacca ccgggtacag tcctaaacga aaaggtacag gaggaaaatc
    16201 aggctgatac atcacgcttc ctccttatgc aggtagattt ctccgcctct ggcacggaag
    16261 ttctccgaca tatccgccat tcccacttca atggtttgcg cggcggcgta atcgcgtact
    16321 tcctggctga ttttcatcga gcagaatttt ggcccacaca tggagcaaaa atgggcgact
    16381 ttgccggact cttggggcag ggtttcatcg tgataagcgc gggcggtaaa cgggtcgagg
    16441 gccagattaa actggtcttc ccagcgaaat tcgaagcggg ctttcgacat ggcgttatcg
    16501 cgaatttgcg cgcccggatg cccttttgcc aggtcagcag cgtgggcggc aatcttataa
    16561 gtgataagcc cctgcttaac atcttcttta ttgggcagac ccagatgctc ttttggcgtt
    16621 acgtaacaga gcatcgcgca gccaaaccag ccaatcatcg ccgcaccaat ccccgacgtg
    16681 aagtggtcat agcccggtgc aatatcggta gttagcggcc ccagagtgta aaacggcgct
    16741 tcgtggcagt gctctaactc ctcggtcata ttgcggcgga tcatctgcat cggcacatgg
    16801 cctgggcctt caatcatcac ctgtacgtca tattcccagg cgattttggt cagttcgccc
    16861 agcgtatgca attcggcaaa ctgcgcttca tcgttggcgt cctgaataga accggggcgc
    16921 agaccgtcgc ccagcgacag cgaaacgtca taagcggcac agatttcaca aatttcgcgg
    16981 aagtgttgat agaggaaatt ttcctgatga tgggagaggc accatttcgc cataatcgaa
    17041 ccgccgcgag agacgatacc ggtcaggcgt ttcgcggtca tcggcacata gcgcagcagt
    17101 acgcccgcat ggatagtgaa gtaatccaca ccttgctcgg cctgttccag cagcgtgtcg
    17161 cggaacgctt cccaggtaag atcttcggcg atcccgttaa ccttctccag cgcctggtag
    17221 atcggcactg taccgatcgg caccggactg ttacgcaaaa tccactcgcg ggtttcgtga
    17281 atatagcgac cggtggagag atccatcacc gtatccgctc cccagcgcgt ggaccatacc
    17341 agcttttcca cttcttcttc gatggaagag gtgaccgccg agttgccgat attggcgtta
    17401 acttttacca ggaagttacg gccaataatc atcggctccg attccggatg attaatgttg
    17461 gccgggataa tcgcacgtcc ggcagcaact tcatcacgga caaattccgc agtgatattt
    17521 tccggcagat gtgcgccaaa gctcattccc ggatgctggt ggcgtaaaac ctcgctacgg
    17581 atgcgctcgc ggcccatatt ctcgcggatg gcgatgaatt ccatttccgg cgtgatgatg
    17641 ccctggcggg cgtagtgcag ttgggtgaca cggcgtcctg ttttggcgcg ttttggcgtt
    17701 aataagccgc taaaacgtag ctcgtcgaga ccatcatctg ccagccgcgt tctggtgtaa
    17761 tcggaactgc gcacggtaag ttcttcggta tcggcgcgcg catcgatcca cggctggcgt
    17821 agttttgcca gcccttgctg cacgttaatg gcgatctgcg gatcaccata cgggccggag
    17881 gtgtcgtaga ccggaatcgc ttcgttttct tcgtactgcg gctgttcttt gctaccgcca
    17941 attagcgtcg ggctaagctg gatctcacgc atcggcacgc gcacgccggg gtgtgtgcca
    18001 gtgatataaa tgcgttttga gttgggaaag gcggtgcctt ccagggtgtc gataaaatgt
    18061 tgggcccggg cgcgttgttc gcggcgggtc agttttgttg cagacatagc tcattccaaa
    18121 aagttaagga cgtggcttgt cagacgacgg atgaagcaag agacgatcgc cgcaaaggcg
    18181 atgcgatagc agattaactc ttgttccctt cgcaggtatt agcctgatca ggttccgcgg
    18241 atcccgaata aacggtctca gccagttaag gcactccgac aagaaattag cccccacaaa
    18301 cggggcgttg aatgtaaatt acgcgttaac agcgcagaac tcaagcagga tgtttgacgc
    18361 gggcggcgtc aagcaccagc agaatcagct tatcttccag cacaaagcgc gcttccagcg
    18421 cctcgccgat gtcagataaa acctgttgaa actcaaggta attatcatga tcgatggcgg
    18481 tttccaggct ggaatcgtag taatccataa tctgttgggt attggcttcg agttgcggcc
    18541 aaatcttcgc ggcgcgcgcg agttgcccgt tgccttccag cttatgaaga atgcgctcat
    18601 aaatactgaa atgtccggca gacaagtaat cgaccaggct ctgacaaaaa tcatcaaggg
    18661 ctttttcgtt tagcctcatg tacgattctt tgccaggctt aatgccaacc agattgtagt
    18721 aagccacgag cagatgctta cgtacatgta gccagcgatc aaccagtttg ttacttcctc
    18781 tgacgcgctc cgtcaggtta tcgagctggt taagcatgat tgactccgca agtttgtatt
    18841 caaaaactgc tcagtgagaa atgtaaaaac catgttaaac atgccagtga tgcaaaggta
    18901 gtgcaagagc tatggatcgt ataattgaaa aattagatca cggctggtgg gtcgtcagcc
    18961 atgaacaaaa attatggttg ccgaagggag aattgccata tggcgaagcg gcaaatttcg
    19021 atcttgtggg tcagcgcgca ctacaaatcg gcgaatggca gggggaacct gtttggttaa
    19081 tacaacagca gcggcgtcac gatatggggt cggtacgtca ggtcattgat ctcgatgttg
    19141 ggctgtttca actggccgga cgtggcgtgc aactggcgga gttttaccga tcgcataaat
    19201 actgtggtta ctgcgggcat gaaatgtatc cgagcaaaac cgaatgggcg atgctgtgca
    19261 gccattgccg tgagcgttac tacccgcaaa tcgccccctg cattattgtt gccatccgtc
    19321 gcgatgattc gatcctcctc gcccagcata cccgccatcg taacggtgtc catacagtac
    19381 ttgccggatt cgtcgaagtg ggcgaaaccc tcgagcaggc agtcgcgcgg gaagtgatgg
    19441 aagagagcgg aattaaagtt aaaaacttgc gttacgtgac ttctcagccg tggccgtttc
    19501 ctcagtcttt aatgaccgcg tttatggcgg aatatgacag cggcgacatc gtgatcgacc
    19561 cgaaagaatt gctcgaggcg aactggtatc gctatgacga tttgccgtta ctcccgccgc
    19621 ccggcaccgt agcgcgccgt ctgatagaag atactgtggc gatgtgtcgg gcagagtatg
    19681 agtgatgata cactgaccgc ctgacgcact aaggaacagc caaaatgacc gaacttaaaa
    19741 acgatcgtta tctgcgggcg ctgctgcgcc agcccgttga tgtcactcca gtatggatga
    19801 tgcgccaggc gggtcgctat ctaccggaat ataaagccac gcgcgcccag gcgggcgatt
    19861 ttatgtcgct gtgcaaaaac gccgagcttg cgtgcgaagt gactttgcaa ccgctgcgtc
    19921 gctacccgct ggatgcggcg atcctctttt ccgatatcct caccgtgccg gacgcgatgg
    19981 ggttagggct ctattttgaa gccggagaag gtccgcgttt tacctcgcca gtcacctgca
    20041 aagccgacgt cgataaactg ccaattccgg acccggaaga tgagctgggt tacgtgatga
    20101 acgcggtgcg taccattcgt cacgaactga aaggcgaagt gccgctgatt ggtttttccg
    20161 gcagcccgtg gacgctggca acctacatgg tggaaggcgg cagcagcaaa gccttcaccg
    20221 tgatcaaaaa aatgatgtat gccgatccgc aggcgctgca cgctctgctc gataaactgg
    20281 cgaaaagcgt cactttgtat ctgaatgcgc agattaaagc cggtgctcag gcagtgatga
    20341 ttttcgacac ctggggcggc gtgcttaccg ggcgcgatta tcaacagttc tcgctctatt
    20401 acatgcataa aattgttgat ggtttactgc gtgaaaacga cggtcgccgc gtgccagtca
    20461 cgctgtttac caaaggcggc ggacagtggc tggaagccat ggctgaaacc ggttgcgatg
    20521 cgctgggcct cgactggaca acggacatcg ccgatgcgcg ccgccgtgtg ggcaataaag
    20581 tcgcgttgca gggtaatatg gatccgtcga tgctgtacgc gccgcctgcc cgcattgaag
    20641 aagaagtagc gactatactt gcaggtttcg gtcacggcga aggtcatgtc tttaaccttg
    20701 gtcacggcat tcatcaggat gtgccgccag aacatgctgg cgtgttcgtg gaggcagtgc
    20761 atcgactgtc tgaacagtat caccgctaag agcctgatcc atcaggctat tttatttgcc
    20821 attttgaggc cgggcagtgc tcaaaatcct cacgtactac gtgtacgctc cggtttttcc
    20881 gcgctgtccg gcctcaaact gccttcaaca attacgcctg ttgaaccagg ctctaaggag
    20941 cgattatgga tctcgcgtca ttacgcgctc aacaaatcga actggcttct tctgtgatct
    21001 gcgaggatcg actcgataaa gatccaccgg atctgatcgc cggagccgat gtcgggtttg
    21061 agcagggcgg agaagtgacg cgagcggcga tggtgctgct gaaatatccc tcgcttgagc
    21121 tggtggagta taaagttgcc cgcatcgcca ccaccatgcc ttacattcca ggttttcttt
    21181 ccttccgcga atatcctgcg ctgctggcag cgtgggagat gctgtcgcaa aagccggatt
    21241 tagtgtttgt cgatggtcat gggatctcgc atcctcgccg tcttggcgtc gccagccatt
    21301 ttggcttatt ggtggatgtg ccgaccattg gcgtggcgaa aaaacggctc tgcggtaaat
    21361 tcgaaccgct ctccagcaaa ccgggcgcgc tggccccact gatggataaa ggcgagcagc
    21421 tggcctgggt ctggcgcagc aaagcgcgct gtaacccgtt gtttatcgct accggccatc
    21481 gggtcagcgt ggacagcgcg ctggcgtggg tacaacgctg catgaaaggc tatcgtctgc
    21541 cggagccaac gcgctgggcg gacgcggtgg cctcggaacg tccggcgttc gtgcgctata
    21601 cagcaaatca gccctaattc aggtaaactg cggataattt ccgtatttga gaactcaaca
    21661 tgttacaaaa cccaattcat ctgcgtctgg agcgcctgga aagctggcag catgtcactt
    21721 tcatggcttg cttatgcgaa cgcatgtacc ccaattacgc catgttctgc cagcaaaccg
    21781 gttttggtga tgggcaaatt taccgtcgta ttctcgatct catctgggaa acgctgaccg
    21841 ttaaagacgc aaaagtaaat ttcgacagcc aactggagaa atttgaagaa gcgattcctt
    21901 cagccgacga tttcgatctg tacggcgttt atccggcaat cgatgcctgc gtggcgttaa
    21961 gtgaactggt ccattcgcgt ttgagtggtg aaacgctcga acacgcggtg gaagtgagta
    22021 agacctccat cacgaccgtt gcgatgctgg aaatgactca ggctggtcgc gaaatgagcg
    22081 atgaagagct taaagaaaac ccagctgtag agcaagaatg ggacattcag tgggaaatat
    22141 tccgactttt agctgagtgc gaagaacgcg atatcgagct gataaaaggc cttagggcag
    22201 acctgcgtga gtcgggtgag agcaatattg gtataatttt tcagcaataa gaccagaaaa
    22261 cgtgatttaa cgcctgattt gtcgtacctg gagtcttccc tttcgccccc cgtctggtct
    22321 acatttgggg ggcgaaaaaa agtggctatc ggtgcgtgta tgcaggagag tgctattctg
    22381 gcatttccgt cgcactcgat gcttagcaag cgataaacac attgtaagga taacttatga
    22441 acaagactca actgattgat gtaattgcag agaaagcaga actgtccaaa acccaggcta
    22501 aagctgctct ggagtccact ctggctgcaa ttactgagtc tctgaaagaa ggcgatgctg
    22561 tacaactggt tggtttcggt accttcaaag tgaaccatcg cgctgagcgt actggccgca
    22621 acccgcagac cggtaaagaa atcaaaattg ccgcagctaa cgtaccggca tttgtttctg
    22681 gcaaggcact gaaagacgca gttaagtaag attgcgtggc agtgaacagt tttaacgaag
    22741 gggtggtttc accccttttg tctttctggc gtcgatcatt gatgctggca ggtgcgctgt
    22801 ttctcactgc ctgtagccac aactcttcac ttcctccctt taccgccagt ggatttgctg
    22861 aagaccaggg cgcggtacgc atctggcgaa aagacagcgg cgataatgtg catctgcttg
    22921 ccgtgtttag cccgtggcgc agtggcgata ccacgacgcg agagtatcgc tggcagggcg
    22981 ataacctcac gctcatcaat atcaatgttt acagcaaacc gccggtgaac attcgcgcgc
    23041 gttttgacga tcgcggcgat ctgagcttta tgcaacgtga atccgatggg gaaaagcagc
    23101 agctttctaa cgaccaaatc gatttatacc gttatcgtgc tgatcagatc cgccagatta
    23161 gcgatgcctt acgtcagggg agagtcgtgc tgcgccaggg gcgctggcat gcgatggaac
    23221 agaccgtgac cacctgcgaa gggcaaacca ttaaacctga tttagattcg caggcgatag
    23281 cgcatatcga gcgccgccag agccgctctt ctgttgatgt cagcgtggca tggctggaag
    23341 cgcccgaagg ttcgcaatta ctgttagtgg caaactctga tttctgtcgc tggcaaccca
    23401 acgagaaaac gttctgattt accagtggcc catacccata tgaccgccac caccgcagcc
    23461 gccgtagccc atgcccattc cggctccgcg cggaataccc gcttcagcca tcgcgatatc
    23521 tcgtttcacc cgtaactcat ctaacgactg acgcaaattc tccatctctt tggcgaccgc
    23581 gttaattttg ctgctatccg gtgggttcgc ggctaacagg gcattgtatt cataacgctt
    23641 cgtcaccagt tgctgttgca gtgcgctgct ttgagcgtaa aagtcattat ggattttctg
    23701 ccacgctgtc tgttgttcgc tggtcaaagg cgcggcattt tgctgccaca taccgtgtcc
    23761 gccgtgagca aatgcagatg tcgatcccat cgccattgct gaaagcgcca tcattaccag
    23821 ggcaattttc gtgttccgtt tcatggttaa tcctccagtg gttgtcttac ttcgggtatt
    23881 gcatcttccg tgccaacgat gaaacgctga tatgacgggt aatctggcat gataaacgag
    23941 taaaaatgac tcgcctgctg cgggtagcga gtcattttta ctcattgaaa cttaagcctt
    24001 tgtgttacag cgcagggtaa gcgctgataa aagatggcat gatttctgct gtcagaaagg
    24061 gatgagcagg caaagaagaa gatgcgtttt atgcaacgtt ctaaagactc cttagctaaa
    24121 tggttaagcg cgatcctccc cgtggtcatt gttgggctgg tagggttgtt tgcggtgacg
    24181 gtgattcgcg attatgggcg cgagactgcc gccgccagac aaacgctgct ggaaaaaggc
    24241 agtgtactta ttcgcgctct tgaatccggc tcgcgcgtcg gcatggggat gcgcatgcat
    24301 catgcgcagc agcaggcctt actggaagaa atggccgggc agcctggagt acgttggttt
    24361 gcggtcacgg atgaacaagg aacaatcgtg atgcatagca actccggcat ggtgggaaaa
    24421 cagctttatt cccctcagga aatgcagcag ttacatccgg gagatgaaga agcgtggcgg
    24481 cggatcgata gcgcagacgg cgagcctgtt ctggaaattt atcgccagtt tcaaccgatg
    24541 tttgctgctg gaatgcaccg gatgcgccat atgcagcagt atgccgcgac accacaagca
    24601 attttcatcg ctttcgacgc cagtaatatt gtgagtgccg aagatcgtga gcagagaaac
    24661 accctgatta ttctcttcgc cctggcgacg gtcttgctgg caagcgtatt gtcattcttc
    24721 tggtatcgcc gctatctgcg ctcgcgccag cttctacaag atgaaatgaa gcgcaaagag
    24781 aagctggtgg cgctagggca tcttgcggca ggcgttgccc acgaaatccg taacccactt
    24841 tcctcgatta aaggactggc gaaatacttt gccgagcgcg cgcctgcagg gggagaagcg
    24901 catcaactgg cgcaggtgat ggcgaaagag gccgaccgtt taaaccgcgt ggtaagcgag
    24961 ttgctggaac tggttaagcc aacgcatctg gctttgcagg cggtggatct caacacgctg
    25021 attaaccact cattacagct ggtaagccag gatgcaaaca gccgggagat ccagttacgc
    25081 tttaccgcca acgacacatt accggaaatt caggccgatc cggacaggct gactcaggtc
    25141 ctgttgaatc tctatctcaa tgctattcag gcgattggtc agcatggcgt gattagcgtg
    25201 acggccagcg aaagcggcgc gggcgtgaaa atcagcgtta ccgacagcgg taagggaatt
    25261 gcggcagatc agcttgaagc catcttcact ccgtacttca ccaccaaagc cgaaggcacc
    25321 ggattggggc tggcggtcgt gcataatatt gttgaacaac acggtggtac aattcaggtc
    25381 gcaagccagg agggaaaagg ctcaacgttc accctctggc ttccggtcaa tattacgcgt
    25441 aaggacccac aaggatgacg cacgataata tcgatattct ggtggtggat gatgacatta
    25501 gccactgcac tattttgcag gctttactgc gcggctgggg ctataacgtc gcgctggcga
    25561 acagcgggcg acaggcgctg gagcaggtgc gggaacaggt ttttgatttt gtgctttgcg
    25621 atgtgcgaat ggcggagatg gacggcatcg ccacgctgaa agagatcaaa gcgttaaacc
    25681 cggcaattcc ggtgctgatt atgactgcgt actccagcgt cgagacggcg gtagaggcac
    25741 tgaaaactgg ggcgctggat tatctcatca agccgctgga tttcgataac ctacaggcga
    25801 cgctggaaaa ggcgctcgcg catacgcaca gtattgatgc tgaaacacct gcggtgactg
    25861 ccagccagtt cggtatggtc ggtaaaagcc cggcgatgca acacctgctc agtgaaatcg
    25921 ccctcgtcgc gccatcggaa gccacggtgc tgatccacgg cgattccggc accggtaaag
    25981 agctggtcgc cagggcgatt cacgccagta gcgcacgtag cgaaaaaccg ctggtaacgc
    26041 tcaactgtgc ggcactcaac gaatccttgc tggaatctga attgttcggt cacgaaaaag
    26101 gggcgtttac tggagccgat aaacgccggg aagggcgctt tgttgaggcg gacggcggca
    26161 cgctgtttct cgatgaaatt ggcgatatct cgccgatgat gcaggtgcgt ctgctgcgtg
    26221 cgattcagga gcgcgaagtt cagcgtgtcg gtagcaacca gactatctcg gttgatgtcc
    26281 ggctgattgc ggcgacccat cgcgatcttg ccgcagaggt gaatgccggg cgttttcgcc
    26341 aggatctcta ctatcgcctg aatgtggtgg cgattgaagt accatcgctg cggcaacggc
    26401 gggaagatat ccctctgctg gctggccatt ttcttcagcg ctttgccgag cgtaatcgca
    26461 aggcggtaaa aggctttacg ccacaggcga tggatttgct gatccactac gactggccgg
    26521 gaaatattcg tgaactggaa aacgcggtgg aacgggcggt ggtgctgctg accggggaat
    26581 atatttccga acgcgagctg ccgctggcga ttgccagtac gccgatcccg ctggggcaaa
    26641 gtcaggatat tcagccgctg gtggaagtgg aaaaagaggt gattctggcg gcgctggaga
    26701 aaacgggcgg caacaaaacc gaagccgccc gtcagttagg gatcacgcgc aaaacgctat
    26761 tggcaaaact gtcgcgttag ttctgctcgc gttcgatggc gcgccaaccg atatctttcc
    26821 ggcagaagca atcatcccag tggatatcgg tcattaaggc gtaggcgcgt ttctgcgctt
    26881 ctgccacggt atgacccagc gcggtgacgc acagtacgcg cccgccgctg gttaccacct
    26941 gctcgtcatc cgccagtttt gtgcccgcgt ggaacacttt gccgtcagcc acttcttcca
    27001 gcggcaggcc gtggatcaca tcgccggtgc ggtaatcgcc cggatatcca cccgcagcca
    27061 tcaccacgct gagagaagca cgttcatccc actctgacgt tttctcgtcc agtttgcctt
    27121 cacaggccgc caggcagagc tcaaccagat cggacttcat gcgcagcata atcggctggg
    27181 tttccggatc gccaaagcgg cagttaaatt caataacctt cggattgccc tgtttgtcga
    27241 tcatcaggcc cgcatagaga aaaccggtgt aggtgttgcc ttccgccgcc atgcctttca
    27301 cggttggcca gatgatacgt tccatggtgc gctgatgaac ttcatcggtt actaccggcg
    27361 cgggggagta agcgcccatc ccgccggtgt ttggtccgtt atctttatcg cctacgcgtt
    27421 tgtgatcctg gctggtagcc atcggcagca catgctcgcc gtccaccatc acgataaagc
    27481 tcgcttcttc gccatcgagg aactcttcga taacgatgcg atgacccgcg tcgccaaaag
    27541 cgttgcccgc cagcatatcg tgaacagccg cttccgcttc ttccagcgtc atcgccacga
    27601 taacgccttt cccggcagcc agaccgtccg ctttaatgac gattggcgcg cctttctcac
    27661 gcagatacac cagcgcaggt tctacctcgg tgaagttctg gtattccgcc gtagggatct
    27721 tatggcgggc caggaaatct ttggtaaacg cttttgagcc ttccagttgg gccgcacctg
    27781 cggttgggcc gaagattttc agcccggcgg cgcggaaggt atcgaccacg cctttcacca
    27841 gcggcgcttc cgggccgacg atggtcagat caatcttttc gttttgtgcg aaatccaaca
    27901 gcgccgggat atcggtcacg ccgatagcaa cgttttgcag cgcgggttcc agcgcagtgc
    27961 ctgcattacc cggagcaaca aaaacagtct caaccagcgg cgactgggcc gctttccagg
    28021 ccagcgcgtg ctcgcgcccg ccgttaccaa tcactaatac tttcatctat tgctccatta
    28081 atggcggaag tggcgcatgt cggtgaagag catcgcaata ccgtgctcgt cggcggcggc
    28141 aatcacttcg tcatcacgga tggaaccgcc aggctggatt acgcaggtca cgcccgcagc
    28201 ggcggcggca tcaataccgt cgcggaacgg gaagaacgcg tcagaagcca tcgaggaacc
    28261 tttcacttcc aggccttcat cggcagcttt aataccggcg attttcgcgg agtacacgcg
    28321 gctcatctgg cccgcgccaa taccgatggt catattgttt ttggcataga cgatagcatt
    28381 ggatttcacg aatttcgcca ctttccagca gaacagtgca tcacgcagtt cctgttcggt
    28441 cggctgacgt tgggtgacga cgcgcagttc ttccgcaccg accatcccca ggtcacgatc
    28501 ctgaaccagc agaccgccgt tcacgcgttt gaaatcaaga cccggaacac gctcgcccca
    28561 ctgaccgcag gtcagaacgc gtacgttctg cttggcggcg gtgattttca gggcttcttc
    28621 gctggcggaa ggcgcaataa tcacttcgac aaactgacga gaaatgatgg cctgtgcggt
    28681 ttccgcatcc agctcgcggt taaaggcaat gatgccgccg aatgcggagg ttgggtcggt
    28741 tttgtacgcg cgatcgtaag catcaagaat ggaattgccg atagctacac cgcaagggtt
    28801 ggcgtgcttc acaatcacac atgccggctc ggcgaactct ttcacgcact ccagcgccgc
    28861 atcggtatcg gcgatgttgt tataagagag ggctttaccc tgaacctggg ttgcggtagc
    28921 aacggaggct tctttcacat tctcttctat atagaaggca gcctgctggt ggctgttctc
    28981 gccgtaacgc atatcctgct tcttaatgaa gttcaggttc agcgtgcgtg ggaagcgacc
    29041 ggcggcttct ttgctttcac cgtggtacgc cggaaccatg ctgccgaagt agttggcaat
    29101 catgctgtcg taggcggcag tgtgttcgaa ggctttgatg gcgaggtcga aacgggttgc
    29161 aagcgtcagc gatccttcgt tgtcatccat ctctttaata atggcgtcgt agtcgctgct
    29221 cttcaccacg attgcgacat ctttatggtt cttagcggcg gagcgcacca tggtcgggcc
    29281 gccgatatcg atgttctcaa ccgcatcttc cagcgagcag ccttcacgag caacagtctg
    29341 ggcgaacgga tacaggttaa caaccaccat atcgataggc tggatctgat gttcttccat
    29401 aatggcatcg tcctggccgc gacggcccag aatgccacca tgtactttcg gatgcagggt
    29461 cttcacgcgt ccatccatca tctccgggaa accggtgtaa tcggaaactt cggttaccgg
    29521 cagacctttt tctgctaaca gacgggcagt gccccctgta gacagcagct ccacaccgcg
    29581 tgcggaaagt gcctgggcga attcgacgat accggctttg tcagaaacac tgagcagagc
    29641 gcggcggact ggacgacgtt gttgcatggt aaatcccctg ggtttgacta ttacagagag
    29701 cgttagctga atttttcgcg aaaaactcag ctaacgcccc taacggggca tccttatttt
    29761 tcgcccgcat tgtaacgaaa acgtttgcgc aacgctcgcg aatttttctc tttcaatggt
    29821 gatcacaatt ttgacagtgg ttaccgtggg aaaaatacag aaattacatt gatgattgtg
    29881 gataactctg tgcgtaaaaa ggtataaagc ggggttttgc tggggaatgc agcagtcagt
    29941 catttttctg caatttttct attacggcct gcggagaact ccctataatg cgcct
//
LOCUS       DADMEQ010000039        29797 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.039, whole genome shotgun sequence.
ACCESSION   DADMEQ010000039 DADMEQ010000000
VERSION     DADMEQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.039"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..218)
                     /locus_tag="JNZ05_001784"
     CDS             complement(<1..218)
                     /locus_tag="JNZ05_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY8565045.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAEVY"
     gene            complement(399..1874)
                     /locus_tag="JNZ05_001785"
     CDS             complement(399..1874)
                     /locus_tag="JNZ05_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY8565046.1"
                     /translation="MIQSTRISLGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNEKTNVKLIPQMNYLMVVVALFFLNA
                     IIFLFMLMKYFTNKQILPTLILSLALLSGLIYLVETIVVIHKPISDSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTFFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHNI"
     gene            complement(2021..3304)
                     /locus_tag="JNZ05_001786"
     CDS             complement(2021..3304)
                     /locus_tag="JNZ05_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HAY8565047.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSSESVSIPTEDISEPMFHQGRGGLRHRVHTGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIEHVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(3417..5351)
                     /gene="yeaG"
                     /locus_tag="JNZ05_001787"
     CDS             complement(3417..5351)
                     /gene="yeaG"
                     /locus_tag="JNZ05_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HAY8565048.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEDNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5787..6533
                     /locus_tag="JNZ05_001788"
     CDS             5787..6533
                     /locus_tag="JNZ05_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA-interacting protein"
                     /protein_id="HAY8565049.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            6623..7384
                     /locus_tag="JNZ05_001789"
     CDS             6623..7384
                     /locus_tag="JNZ05_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAY8565050.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLCYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLVQAGRLRNGLLKNAVVNEIAHV
                     QQNAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(7435..8319)
                     /locus_tag="JNZ05_001790"
     CDS             complement(7435..8319)
                     /locus_tag="JNZ05_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HAY8565051.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGSVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(8403..9398)
                     /gene="gapA"
                     /locus_tag="JNZ05_001791"
     CDS             complement(8403..9398)
                     /gene="gapA"
                     /locus_tag="JNZ05_001791"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY8565052.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9740..10153
                     /gene="msrB"
                     /locus_tag="JNZ05_001792"
     CDS             9740..10153
                     /gene="msrB"
                     /locus_tag="JNZ05_001792"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAY8565053.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            10195..10467
                     /locus_tag="JNZ05_001793"
     CDS             10195..10467
                     /locus_tag="JNZ05_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HAY8565054.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            10837..11913
                     /locus_tag="JNZ05_001794"
     CDS             10837..11913
                     /locus_tag="JNZ05_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY8565055.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            11940..13319
                     /locus_tag="JNZ05_001795"
     CDS             11940..13319
                     /locus_tag="JNZ05_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8565056.1"
                     /translation="MEQITKPHCGVRLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESCGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYLLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAASIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            13336..14381
                     /locus_tag="JNZ05_001796"
                     /pseudo
     CDS             13336..14381
                     /locus_tag="JNZ05_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            14402..15238
                     /locus_tag="JNZ05_001797"
     CDS             14402..15238
                     /locus_tag="JNZ05_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAY8565057.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            15243..16191
                     /locus_tag="JNZ05_001798"
                     /pseudo
     CDS             15243..16191
                     /locus_tag="JNZ05_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
     gene            16201..17181
                     /gene="ydjG"
                     /locus_tag="JNZ05_001799"
     CDS             16201..17181
                     /gene="ydjG"
                     /locus_tag="JNZ05_001799"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HAY8565058.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDPALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            17318..18076
                     /locus_tag="JNZ05_001800"
     CDS             17318..18076
                     /locus_tag="JNZ05_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY8565059.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            18194..19552
                     /locus_tag="JNZ05_001801"
     CDS             18194..19552
                     /locus_tag="JNZ05_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8565060.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFLRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(19645..20286)
                     /gene="pncA"
                     /locus_tag="JNZ05_001802"
     CDS             complement(19645..20286)
                     /gene="pncA"
                     /locus_tag="JNZ05_001802"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HAY8565061.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(20297..21313)
                     /gene="ansA"
                     /locus_tag="JNZ05_001803"
     CDS             complement(20297..21313)
                     /gene="ansA"
                     /locus_tag="JNZ05_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAY8565062.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRL
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNVLAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(21480..23336)
                     /gene="sppA"
                     /locus_tag="JNZ05_001804"
     CDS             complement(21480..23336)
                     /gene="sppA"
                     /locus_tag="JNZ05_001804"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAY8565063.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            23497..24048
                     /locus_tag="JNZ05_001805"
     CDS             23497..24048
                     /locus_tag="JNZ05_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HAY8565064.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            24165..25208
                     /gene="selD"
                     /locus_tag="JNZ05_001806"
     CDS             24165..25208
                     /gene="selD"
                     /locus_tag="JNZ05_001806"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HAY8565065.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            25213..27174
                     /locus_tag="JNZ05_001807"
     CDS             25213..27174
                     /locus_tag="JNZ05_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAY8565066.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIEQDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSVAIA"
     gene            27301..28341
                     /locus_tag="JNZ05_001808"
     CDS             27301..28341
                     /locus_tag="JNZ05_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY8565067.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIAVLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(28458..>29797)
                     /gene="gdhA"
                     /locus_tag="JNZ05_001809"
     CDS             complement(28458..>29797)
                     /gene="gdhA"
                     /locus_tag="JNZ05_001809"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAY8565068.1"
                     /translation="QTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPKYR
                     QMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVNLS
                     ILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQALMTELYRHLGADT
                     DVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYFTE
                     AMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARAITASDSSGTVVDESGFTKEK
                     LARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDTAHQLI
                     ANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLGWK
                     AEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
BASE COUNT         7666 a         6860 c         7571 g         7700 t
ORIGIN      
        1 cagtacacct cagcgagagc gcgcatcaca tgcgcggcaa tactctgatt ctgatactta
       61 tcataaagaa aggaggagtc tgctttctgc acgatatagc gcagataggc actcattgct
      121 ctgtcgctgg cgacaagttc aaaaataagg aaggaagatg tcagcacaat gacacttgcg
      181 gcgataaaat gccggagcat tctatggtgc aatttcatga taaatggcac tccttcggtt
      241 agtgaaacga gaaaaccaga taaagttcga ttgcggcctg gcacaggggc gctaattata
      301 cttatctctg cgccgctggg gaggggaaat ttaggggtaa tagtaataaa ttgtatgcaa
      361 aagccacgca aaaagcatgg ctctgaatat ttttgcactt aaatattatg aataatcact
      421 ttgttgcccc cctcgctttt cgcttcgcgt aaggctttat ctgcttccgt catgacaagc
      481 gagatattac gagtgtcacc cgttgaaaaa aacgtgccaa tactgatggt catttgttca
      541 ggaacatcat atctgttact gaagccggtc aggagttcga catttttacg catacgttcc
      601 gcaatgattt ttgcctgggc actattgagt tcggtaaata gcaagccaaa cacctcaccc
      661 tccagtcgcg ctaaaatatc atcggggcgg atgctttgct ggataatatc gacaattgaa
      721 accagcactt tatcacccac acgatgtccc cattgggcat tgatgcgttt gaactgatta
      781 atatcaacca gcatgacaca gaagtcctta acaacaggtc gcgacaataa tgactctacg
      841 ctgttgaaaa agtagcgccg attataaata ttggtcagca catcatgaac tgccagtttg
      901 ctggagagtt gcagttcctg aaaaatgtta taaatcagaa aagacacaac aaacagttta
      961 ctggagactt caatcccgcg actgatgtac cagatggtgt aattatactc atccagagtc
     1021 agtagaagta agttgcagca tagcgatgcc agacataata atgctattaa tggccatagt
     1081 tcgctggcta atcgtgtacg catgataata aagaacagta aaaatgccca cagacaaacc
     1141 aatatttttg tatagttgat tccccaggtc gcagtatggt tgtcaggaca gtaatcgacg
     1201 acatacaaag aatagtcagc gttataactg ctcagattgt gtgccagaag gggaaaaact
     1261 aaaaaaggaa tcagcgccag caacagaatg ccagtttttt tcttattgtt gtcaaggatg
     1321 ttgtctttac cataacaaaa gagcgccaac gaggttaaac aaataaaact gagttggcgg
     1381 aaaatataga aaatagaaac atcattcgac tttgtctgga tcagtgtact gtcgctgata
     1441 ggtttatgga taacaacaat agtttcaact aaatagataa ggccacttaa aagtgcaaga
     1501 cttaaaatga gtgttggtag aatttgtttg ttagtgaaat atttcattaa catgaaaaga
     1561 aaaatgatgg cgttaaggaa aaacaaagcc acaacgacca tcagataatt catttgtggg
     1621 atgagtttta cattcgtctt ttcgttatat aatgtggtga tgcaaaataa aattaacgtc
     1681 agcaataaac tgaaaataaa aaagctttga gcttttaatt tttgtgtgcc catagaaacc
     1741 ataaaaagga gagcaatgtg agtgctggat tttattgatg gcagagatat atagttttga
     1801 ccaagatcaa ttttcgttaa cgataaaaaa tatttaaaga ataaccccag ggaaatacgt
     1861 gttgactgaa tcatttttcc gaatgattgt tttctggaac taaattgtgt aaaggttatg
     1921 ctgtcatatt taaagcgatt gtaagctaat gtatgtaata aatgagataa tttacagtaa
     1981 gctaaaaatc agccaggcga taatgacccg gctgatagtt ttagtcttta gctgttgcat
     2041 tttgtttatg aaacagttca cggaacaccg gataaatatc atcctggtcg cggatgtgct
     2101 gcatcgcaaa gttgtcgaaa gtagattgca gatgctcata ttctcgccac aatgtctgat
     2161 gtgcacgacg ggtaatttcg atatagctgt aataacgaac aacaggtaat aattttttcg
     2221 ccaggatttc atggcaaagc ggagagtcat cggcccagtt atcgccgtcc gatgcttgtg
     2281 cagcgtaaat attccactgt gccgggttat aacgctcttt cactacctca tccatcagtt
     2341 tcagggcgct ggaaacaatg gtgccgcctg tttcctgcga gtagaaaaac tcatgttcat
     2401 cgacttcttt cgcctgggta tgatggcgga tgtatacgac ttccacgttc ttatacgttc
     2461 tgctgaggaa cagatacagc agaatataaa aacgcttagc catatcttta gtggattgat
     2521 ccattgaacc ggaaacgtcc atcaggcaaa acatcactgc ctggctggag ggatcgggcc
     2581 gcttctcgta gttcttgtaa cgtaaatcga aggtgtcaat aaaagggacg tgttcaattt
     2641 tggcacgtaa ttctgcaatt tctttgcgca gacgttcctc ttccagcagc tgcgcaggtt
     2701 cactgttgct gatgatggcc aaattctctt ccagtgcatg aagttcccgc cgcttgcctg
     2761 ccgtcatggc tgtgcgtcgc gccagtgagt tctgcaagga acgcacaacg ctgatattgg
     2821 ctggaacgcc gttagcggta taacccgccc gatgcgtttt atattcggtc agctggcgtt
     2881 gttggttttg tttcagattc ggtaaggcca aatcttcaaa gagcagatca agatactcat
     2941 ctttcgaaat ctgaaagaca aattcatcct gaccttcacc atcctggctg gcctggccct
     3001 gaccactgcc ggaacctccg ccgccaccct ggggacgttc aattcggtcg ttctgaacga
     3061 aatggtcatt gcccgtatgc acgcggtggc gcagaccgcc acgcccctga tgaaacattg
     3121 gttcgctaat atcttccgtg ggaatggata cggattcgct gctgtcgacg tcagtcaccg
     3181 aacgcttatt aatggcctcg gagatcgact gtttaatttg cgctttataa cggcgtaaaa
     3241 aacgctggcg attcaccatg cttttgtttt tgccgttcag acgccggtca ataaaccagg
     3301 tcatatgccc cccgtactac atttgccaac tgtatgctac gcctgtgttg ccgcttctca
     3361 ggcccacggt tcgctatcgt tgtaggcccg gtagcatgtg taccgggcca gggttgttaa
     3421 gacgatttac gtacgcgcaa ataccattcg cacagtaaac gcacctgttt acgggtgtag
     3481 cctttttcca tcatacggtc gacaaagtcg tcgtgtttct tctgctcgtc ggttgacgtt
     3541 ttggcgttaa acgagataac cggcaacagc tcctcggtat tggagaacat tttcttctcg
     3601 ataaccgtgc gcagtttttc atagctggtc cagttcggat tgcgtccgct gttattcgct
     3661 ctggcgcgca gtacgaagtt aacaatctcg ttgcggaaat cttttggatt actgatcccc
     3721 gccggtttct cgattttctc cagctcggcg ttaagagact cgcggtcaaa cagctgcccg
     3781 gtatccggat cgcgatactc ctgatcctga atccagaaat ccgcgtaggt aacataacgg
     3841 tcgaaaatgt tttgcccata ttcggaatag gattcaaggt aggccgtctg gatctctttg
     3901 ccgataaact cggcatattt cgggatcaga taacctttca ggaactccag atagcgttct
     3961 gcctgctctt gcgggaactg ctcgcgctcg atctgctgtt ccaggacgta gaacagatgg
     4021 accgggtttg ccgccacttc tacatgatcg aagttgaaca cgcgggagag gatcttaaac
     4081 gcaaaacgcg tcgacagacc gttcatccct tcatcgacac cggcgtagtc acgatattcc
     4141 tgatacgact tggctttcgg atcggtgtct ttcagacttt cgccatcata aacccgcatc
     4201 tttgaataaa tgctggagtt ttctggctct ttcaggcgcg aaagaatgga aaaacgtgac
     4261 agtgtttcga gcgtgccagg ggcgcatggg gcgtgagtca attcactgtg attaagcaat
     4321 ttctcgtaga ttttgatctc ttcggaaatg cgcaagcaat acggcacctt cacgatgtaa
     4381 acacggtcga ggaaggcttc gttgtttttg ttattacgga aagtgaccca ttcggactcg
     4441 ttcgagtggg cgagaataat cccgttgaac ggcagggcgg agataccttc cgtcccgttg
     4501 tagttatctt cctgagtggc ggttaacaag ggatgcagca cttttatcgg tgctttaaac
     4561 atctcaacga attccatgat cccctgattg gcgcggcaca gcgcaccgga atagccgtag
     4621 gcgtccgggt cattctgcgc gtagtgttcg agtttacgaa tatcgacttt cccaaccagc
     4681 gcggagatgt cctggttgtt ctcatcaccg ggttccgttt tggcgatagc aatttgttgc
     4741 agaattgacg gccagacctt cactacccgg aacttagtga tatcgccacc aaattcatgc
     4801 aggcgttttg ccgcccatgg cgacatgatg gtgccgagat aacggcgagg aatgccatac
     4861 tctttttcca gaatctgagc atcttcctgc ggattgaaaa gacagaacgg atgatcgttg
     4921 accgggctac gctcaccgtt ggcgctcaat acataaatcg gtacgagctg cattaatgat
     4981 ttcagtcgct cagcaagcga tgatttaccc ccacccacag gccccagcag atacaggatt
     5041 tgtttcttct cttccagccc ctgagccgcg tgtttcagat aagagacaat ctgttcaatc
     5101 gcgtcttcca tgccgtaaaa ctcttcaaat gccggataac gtgcaatgac ccggttagaa
     5161 aagagtcgag aaagtctggg ttcctgggct gtatcgacca tgacaggctc accgatagcc
     5221 atcaatagcc gctcagccgc gttggcataa gcactgcgat cttgccgaca agtggtaaga
     5281 aactcctgca gtgtgaactc ttcgtccttg gcagcttcat atcgctggcg atagtgatcg
     5341 aatatattca tggtatgccg tcctttcgtt ttttagcaca ggttaagagc cgttcatatg
     5401 aatagtagag gctcccggaa gaggtaaact gaacgacgtc tcttgctaaa acacagcaac
     5461 tctcatgcca ttttatcgac gatgagaaca acgtgcccgg tgatacacct tcaaaattaa
     5521 gcgtagatgg cagatggaaa acttgcctgc acggctttct taatttcaat ggcatatcaa
     5581 caactcatcc ataaattttg cataattaat gtaaagacca ggctcgccag taacgctaaa
     5641 tccatttggc tgtaagcgcg gtgtcatccg cggcaggaaa attaaacagt tactttaaaa
     5701 aatgaaaacg taaaaaggtt gggtttcgat gtattgacgg gtaaactttg tcgcccgcta
     5761 aacatttgtt taaggaatga ttaattgtga ccaaactcaa acttctggca cttggagtgc
     5821 ttatcgcaac gtctgcaggc gtagcgcacg ctgaaggtaa attttccctg ggcgcaggcg
     5881 taggtgtcgt tgagcaccca tataaagatt acgataccga tgtttaccca gtaccggtaa
     5941 tcaactatga aggcgataac ttctggttcc gtggcttagg tggtggttac tacctgtgga
     6001 atgacgcaac ggataaactt tcaattaccg cttactggtc gccgctttac ttcaaagcga
     6061 aagacagtgg cgatcaccaa atgcgtcacc tggatgaccg taagagcacc atgatggctg
     6121 gtctgtctta tgctcacttt acccagtacg gttacctgcg taccaccctg gctggcgata
     6181 ccctggataa cagcaacggc attgtctggg atatggcttg gttgtatcgt tacaccaacg
     6241 gtggcctgac cgtgactccg ggtattggtg tgcagtggaa cagcgaaaac cagaacgaat
     6301 actattatgg cgtatcgcgc aaagagtccg ctcgcagcgg tctgcgtggg tataacccga
     6361 atgacagctg gagcccttac cttgagctga gcgccagcta caacttcctc ggcgactgga
     6421 gtgtttacgg taccgcgcgc tacacccgtc tgtctgatga agttactgac agcccgatgg
     6481 tggataaatc ctggactggc ctgatttcta ccgggatcac ctacaaattc tgataatgca
     6541 tctttccagg gcattttgtg cataaaaata gggcgtcacg ccctgttttg cattacggtg
     6601 gtgcaatacc tgggaggaaa caatgcaaca aaaaatgatt caatttagtg gcgatgtctc
     6661 actgccagcc atagggcagg gaacatggta tatgggcgaa gatgccagtc agcgcaaaac
     6721 agaagttgct gcactacgcg caggcattga actcggttta accctcattg ataccgccga
     6781 aatgtatgcc gatggcggtg ccgaaaaggt ggttggggaa gcattaaccg gtctgcgaga
     6841 gaaggtcttt ctcgtctcta aagtctatcc gtggaatgct ggcgggcaaa aagcgataaa
     6901 tgcatgcgaa gccagtttac gccgtctcaa tactgattat ctcgatctct acttattaca
     6961 ctggtctggc agtttcgctt ttgaagagac tgttgctgcg atggaaaaat tgatcgccca
     7021 gggaaaaatc cgtcgctggg gcgtttctaa cctttgttat gccgatatgc aggaactctg
     7081 gcagctgccg gggggaaatc agtgtgccac taatcaggtg ctttaccatc tcggttcacg
     7141 agggattgag tacgatctac tcccctggtg ccagcaacag cagatgccgg tgatggctta
     7201 cagtccatta gtccaggccg ggcggttgcg caatggactg ttaaaaaacg cggtagtcaa
     7261 cgaaattgca catgtccaac aaaatgcagc tgcgcttgag gtcgaacttt cgtcagcgga
     7321 attagctatg ctggataagg catatccggc accaaaagga aaaactgcgc tggatatggt
     7381 gtgatgttga tacgtgccgg atgcatcgca cccggcacac aacattaacg caaattaacg
     7441 tttggcaacg cgaatggatt gcgccagatg tgcaggtttc tctttggtga ctttttgcgt
     7501 ttctgaagcg taagccgttt ctacacaaac aaaggtcttg tagccatcgt ccggcatgtc
     7561 gcccatgcta actgaaagcg ccggtcccgg gttccagccg acaacgttca gatgatgctg
     7621 gtggcctacg gcgataatac gattcagcgc ttcatcatta atcacgctgc aatcttgcgg
     7681 attcagatac acgcgatcgg tacggtcagg gaaggtctga ataccgtcgg tcagtacatc
     7741 ttctttcgcg tcattcactt tatcaatgaa gcgatcgccc agcccactga cgcttacctt
     7801 agcgatatca cccacgttaa agtaggtatg cagggcagag gtggtttcaa attcgccatg
     7861 tgattcaaga tcgatttcac aggttttacc cacgcggaaa tgcgctaaca gcgtaaagtc
     7921 gtgcggccag aattttttcg tctcttcgct ttgtgtcaat tcaaaagtca gcgctacgcc
     7981 atcagcatct tcatgatgtg atttcagcgt ccacggcagg ttgcgggcaa aaccgtgcgc
     8041 aggcagacct tgttgtgccg ccggaccaaa ccacggccag caaaccggta cgctaccgcg
     8101 aatagcgacg ccatttttga acggtgtgtt gttgctcaac cacagaactt cttcttcacc
     8161 cgcaggtttc cacgagagaa ggtgtgcgcc ctgtaatgca aaagaggctt ttacctgggg
     8221 atgatcgacc acaatgaggt ccagttcatc cagtttacga cgggagagga caggggagat
     8281 ttgttcgatg accggaaggg caaaaatttt cttaatcatg acgcagtcct ttaacttcat
     8341 tttatcaggt aaaaaaaaga gcgaccgaag tcgctctttt tagatcacag tgtcatctca
     8401 acttatttgg agatgtgagc gatcaggtcc agaactttgt tggagtaacc ggtttcgttg
     8461 tcgtaccaag ataccagttt cacgaagttg tcgttcagag cgataccagc tttagcatcg
     8521 aacacggaag tgcaaacttc gccgttgaaa tcggtagata ctacgtcatc ttcggtgtag
     8581 cccagaacgc ctttcatttc gccttcagca gcagctttaa cggcagcttt gatctgctcg
     8641 taagttgcag ctttttccag acgaacggtc aggtcaacta cggatacgtt cggggtcgga
     8701 acgcggaacg ccataccagt cagtttgcca ttcagttctg gcagtacttt acctacagct
     8761 ttagcagcac cggtagagga cgggatgatg ttctgggaag cgccgcggcc gccgcgccag
     8821 tctttgtgag acgggccatc aacggttttc tgagtagcgg tagtagcgtg aacggtggtc
     8881 atcagacctt cgatgatgcc gaagttatcg ttgataactt tagccagcgg agccaggcag
     8941 ttggtggtgc aggaagcgtt ggaaacgatg tcctggccag catatttgtc gaagttagcg
     9001 cctttaacga acatcggagt gttgtcttta gacggaccag tcataaccac tttcttcgca
     9061 ccagcggtga tgtgtttacg agcagtttcg tcagtcagga acagaccagt tgcttcagcg
     9121 acaacgtcaa cacccacttc gtcccatttc aggttagccg gatcacgttc agcggtaaca
     9181 cggatttttt taccgttaac gatcagatga ccgtctttca cttcaacggt accgtcgaaa
     9241 cggccgtgag tggagtcata tttcagcatg tatgccatgt aatcagcgtc taacaggtcg
     9301 ttgattgcaa cgatctcgat gtcagaacgt ttctgagcag cacggaaaac aatgcgaccg
     9361 atacggccaa aaccgttgat acctactttg atagtcatat attccaccag ctatttgtta
     9421 gtgaataaaa ggttgcctgt aaaattacaa aaaccttacg cagcgtcaag cggaatcgtg
     9481 tcaatcattg cgacaaatca atcctgtgcc taagcattac gcggctgact cgcctcactc
     9541 ttcctttggg cttgagacca catggggtcg gcgccccgaa ttttaaaggg caattacgat
     9601 aaaaatgtga ttttcatcac gatttaggtg cagccatttc gtcgagatca agtttaggac
     9661 aaaattttgc cagttggtgt taatgttttg ttagaatcag tcacgtaatg tgagcacgat
     9721 taaagtgaga tgtgagtaaa tggcgaataa accttcggca gaagaactga aaaaaaattt
     9781 gtccgagatg cagttttacg tgacgcagaa tcatgggaca gaaccgccat ttacgggtcg
     9841 tttactgcat aacaagcgtg acggcgtata tcactgtttg atctgcgatg ccccgctgtt
     9901 tcattccgaa accaagtatg attccggctg tggctggccc agtttctacg aaccggtgag
     9961 tgaagaatcc attcgttata tcaaagactt gtcacatgga atgcagcgca tagaaattcg
    10021 ttgcggtaac tgtgatgccc atctggggca tgtctttccc gacgggccgc aaccaacagg
    10081 cgaacgttat tgtgttaact ctgcttcttt acgcttcact gatggcgaaa acggcgaaga
    10141 aatcaacggt tgaaaaaacg attcagcaac ttattccaca ggagcggaaa aattatgaat
    10201 cttgatgaca ttatcaacag catgacgcct gaggtatacc agcgtttgtc gaccgccgtt
    10261 gaactgggga aatggcctga tggcgttgcg ttgaccgagg aacaaaaaga gaattgcctg
    10321 caactggtga tgctgtggca agcccgccat aataccgaag cacagcatat gacaattgac
    10381 acaaatggtc agatggtcat gaagagcaaa cagcagttaa aagaagattt tggtatctca
    10441 gcaaagccta ttgccatgtt taagtaagtg gttcatcccc tttttgccgc ggctatgaag
    10501 gggatagcca acaatgaccc cacgactcgt ctcaattatc tcgacccgac ctggctaagt
    10561 cccagtccga tctcttgcag tcgcgttcac gcctgaatga cttctcctga acttaagtct
    10621 cagacctatt tggccggtaa tccctctcga atttgctctt tttgcgtccc gccttccacc
    10681 aacctgtaac agacccagcc attttctgcg taattgtgat ctaagttgta aaatgcaggt
    10741 cattatctgt gttgtgcaga tttaatgttg acctcaaaaa tcatgtgggt aagtatgatg
    10801 gccatctaag caaaacaaaa cacagaggat gacgcaatga aagcactggc tcggtttggc
    10861 aaggcctttg gcggctacaa gatgattcat gtcccacaac ccatttgtgg cccggaagac
    10921 gtagtgattg aaattaaagc cgcggcaatc tgcggcgcag acatgaagca ctacaatgtc
    10981 gatagcggtt ctgatgagtt taactctatc cgcggccatg agttcgcagg ttgtattgcg
    11041 caggttggtg aaaaagtcaa agactggaaa gtggggcaac gcgtcgtgtc ggataacagc
    11101 ggtcacgttt gcggcgtttg tccggcctgt gaacagggtg attttctgtg ttgtacagaa
    11161 aaagtaaacc ttggtctgga taacaatacc tggggcggtg gtttttccaa atattgtctg
    11221 gttcctggtg aaattctcaa aattcatcgt catgcgttgt gggaaatccc tgatggtgtt
    11281 gattatgagg acgcagccgt acttgaccct atctgtaatg cctataaatc catcgcgcag
    11341 caatcgaaat tccttcctgg tcaggatgtt gtcgtcattg gcactggccc actcgggctg
    11401 ttttccgtac aaatggcgcg gattatgggg gcggtaaata tcgtcgtcgt tggtctgcaa
    11461 gaagatgtgg cggtccgctt cccggttgca aaagaactgg gggcgacggc agtagtaaat
    11521 ggttctaccg aagatgtggt ggcgcgctgc cagcaaattt gtggcaaaga taatctggga
    11581 ctggtgattg aatgctccgg tgccaatatc gcactgaaac aagccatcga aatgctccgc
    11641 ccgaatgggg aagtggtacg cgttggaatg ggcttcaaac ctcttgattt ctcgattaat
    11701 gacattaccg cctggaataa aagcatcatt gggcatatgg cctatgactc cacctcatgg
    11761 cgtaacgcta tccggctatt agccagcggc gctatcaaag tcaaaccgat gatcacgcat
    11821 cgtatcggcc tgtcgcaatg gcgcgaaggg tttgatgcga tggtcgataa aaccgcaatc
    11881 aaagtgatca tgacttacga ctttgatgaa taactactct cctgaaggaa cgttatcgca
    11941 tggaacagat aacaaaaccg cattgtggtg tacggctgga tcgcttaccg gattgccgct
    12001 ggcattcgtc aatgtttgcc atcgtcgcgt ttggcttgct tgtctgctgg agtaatgccg
    12061 ttggtggctt gatcctcgcg cagctgaaag cgttgggctg gacagataat tccaccacag
    12121 ccacattctc agcaatcacg accgccggaa tgtttctcgg tgcgctggtg ggcggcatca
    12181 ttggcgacaa aaccggtcgc agaaatgcgt tcatcctcta tgaggccatt catattgcct
    12241 cgatggttgt cggtgctttc tcaccgaata tggattttct tattgcctgc cgttttgtga
    12301 tggacgttgg gctgggagct ttactggtta cactgtttgc tggtttcacc gaatatatgc
    12361 ccggtagaaa tcgcggaacg tggtccagtc gggtttcctt tattggcaac tggtcatatc
    12421 cgctctgctc actgattgcg atgggactca cgccgctgat tagtgcagag tggaactggc
    12481 gggtacaact gcttattcct gcaatattgt cgcttatcgc tacggcgctg gcctggcgct
    12541 actttcctga atccccgcgc tggctggaat cgtgcggacg gtatcaggaa gccgagaaag
    12601 tcatgcggag tatagaagaa ggcgtcatac gccagacggg taaacctttg ccgcctgtgg
    12661 ttattgctga tgacggtaaa gcgccacaag cggtgccgta ttcagcctta ctgacaggag
    12721 tattactgaa acgcgtgata ttaggctctt gtgtgctgat taccatgaac gttgtgcagt
    12781 acacactaat taactggttg ccaacaatat tcatgaccca ggggattaat ttaaaagact
    12841 cgattgtttt aaataccatg agtatgtttg gcgcgccatt tggtattttt attgccatgc
    12901 tggtgatgga taaaattccg cgtaaaacaa tgggtgtggg gctattaatc ctgattgcgg
    12961 tgctcggata tatctattta ctgcaaacca gtatgttgct cataacgctg attggtttct
    13021 tcctgattac tttcgtctat atgtacgttt gctatgcctc ggcagtgtat gtccctgaaa
    13081 tctggccgac agaggccaaa ctccgtggct ccggtctggc gaatgcggta gggcgaatca
    13141 gtggtattgc cgcaccttat gccgttgcag tgctgctcag tagttatggt gtaacgggag
    13201 tctttattct tctgggggct gcttcaatta ttgtcgcaat tgctatcgcc accattggaa
    13261 ttgaaaccaa aggtgtctcc gttgaaagtt taagtattga tgcagtagcc aataaataat
    13321 acgggtggat tcaaaatgaa aaattcaaaa gcaatattgc aggtgccggg cacaatgaaa
    13381 attatttcag cagaaatacc agtgcctaaa gaagatgaag ttttgattaa agtagaatat
    13441 gtcggtattt gtggttcaga tgtacatggt tttgaatcag gcccgtttat tccgcctaaa
    13501 gacccaaatc aagaaattgg cctgggtcat gaatgcgccg ggacggttgt ggctgtggga
    13561 agccgcgtgc gcaaatttaa accgggggat cgggtaaata tcgaacctgg cgttccttgc
    13621 ggtcactgtc gttactgtct ggaaggcaaa tataacatct gcccggacgt tgattttatg
    13681 gcgacacaac ccaactaccg cggcgcatta acgcactatc tgtgtcatcc ggagagcttt
    13741 acttacaaac tgcccgacaa tatggacacg atggaagggg cgctggtgga gcctgccgca
    13801 gtcgggatgc atgccgcgat gctggcagat gttaaaccgg gtaagaagat aattattctg
    13861 ggagcaggtt gtattggttt gatgacgttg caagcgtgca aatgcctggg cgcaacggaa
    13921 attgccgtcg ttgatgtact ggaaaaacgt ctggcaatgg gggaacagct tggtgcgaca
    13981 gtggttatta atggcgcaaa agaggacact attgcacgct gtcagcaatt taccgaagac
    14041 atgggcgcag atattgtttt cgaaacagcg ggttctgcgg tcaccgttaa acaggcacct
    14101 ttaatctggt aatgcgcggc ggtaaaatta tgattgttgg tactgtaccc ggcgattcgg
    14161 caatcaattt cctcaaaatc aatcgcgaag tcactatcca gacggtattc cgctatgcca
    14221 atcgttatcc ggtcacgatt gaagctattt cttcagggcg attcgatgtg aaatcgatgg
    14281 tgacgcatat ttacgattat cgggatgtac aacaggcatt tgaagagtca gttaacaaca
    14341 aacgcgacat tattaaaggc gttattaaaa ttagcgatta attaatacgg gagaacagaa
    14401 catgctcgca gatatcagat attgggaaaa cgatgccaca aataaacatt atgcaattgc
    14461 ccattttaac gtatggaatg cagaaatgtt gatgggcgtt aaagacgcag ccgaagaagc
    14521 gaaatccccg gttattattt cttttggtac aggttttgtc ggtaacacct catttgaaga
    14581 tttctctcac atgatggtat caatggcaca aaaagcaacg gtgccggtaa taactcattg
    14641 ggatcatggt cggagtatgg agattattca taacgcctgg actcatggta tgaattcatt
    14701 aatgcgtgat gcttctgcat ttgatttcga agaaaatatt cgtttaacca aagaggctgt
    14761 tgatttcttt catccgctgg gtattccggt agaggcggaa ttagggcatg tcggtaatga
    14821 aaccgtttat gaggaagcgc tggcgggcta tcactatacc gatcctgacc aggcggctga
    14881 atttgttgaa cgtacgggct gtgactcatt ggctgtcgcc atcggcaacc agcatggggt
    14941 ttatacgtca gagccgcaat tgaactttga ggtcgtcaaa cgcgtacgcg atgctgtttc
    15001 tgttccgctg gttttgcatg gcgcatcggg gatcagtgat gccgacatta aaactgcaat
    15061 ttcgttgggt attgcgaaaa tcaacattca tacggagctc tgtcaggccg caatggtcgc
    15121 agtgaaagaa aaccaggatc agcctttcct gcatttagaa cgtgaagtac gtaaagctgt
    15181 aaaagaacgg gcattggaaa aaattgaact gttcggttct gatggcaaag cggaataatg
    15241 atatggataa tctcgacgtt atttgtatag gtgccgctat tgttgatatt ccattacaac
    15301 cggtcagtaa aaatatcttt gatgtagatt cttatcctct tgaaagaatc gcaatgagca
    15361 ccggggggcg atgcaataaa tgaagcaaca attatttctc gtctggggca tcgtaccgca
    15421 ttaatgagcc gcattggtaa agatgcctca gggcaattta ttctcggcca ttgtcgcaaa
    15481 gagaatattg atattcaaag cctgaaacag gacgtcagca tagatacatc tattaacgtt
    15541 ggactggtga cggaggatgg cgagcggacg tttgtcacga accgtaacgg cagtttgtgg
    15601 aaattaaata ttgacgatgt tgattttgcg cggtttcctc aggcgaaatt attatcactg
    15661 gccagtattt tcaacagtcc tctattggat ggtaaagcgc taacagaaat ttttacgcaa
    15721 gcgaaagccc ggcagatgat catttgtgcc gatatgatca aaccgcggtt gaatgaaacg
    15781 ctggatgata tttgcgaagc attgagttat gtcgattatc tgtttccaaa ttttgccgag
    15841 gcaaaattac tcacagggaa agagacactg gatgaaattg ctgactgctt tcttgcgtgc
    15901 ggcgtaaaaa cggtggtgat taaaacgggt aaagacggct gctttatcaa gcgtggtgac
    15961 atgacgatga aggtgccggc ggtcgcagga ataaccgcca tcgacaccat tggcgcgggc
    16021 gataactttg cttcaggttt tattgcggca ctgttagaag gcaaaaatct gcgtgaatgc
    16081 gcacgctttg ccaatgcaac ggcggctatc tcggttttaa gcgtcggtgc caccaccggc
    16141 gtaaaaaaca gaaagctggt cgaacaattg ctggaagaat acgaaggata atgaaggcaa
    16201 atgaaaaaga tacctttagg cacaacggat ataacgcttt cgcgaatggg gttggggaca
    16261 tgggccattg gcggcggtcc tgcatggaat ggcgatctcg atcggcaaat atgtattgat
    16321 acgattcttg aagcccatcg ttgtggcatt aatctgattg atcctgcgct aggatataac
    16381 tttggcaata gtgaagttat cgtcggtcag gcgttaaaaa aactgccccg tgaacaggtt
    16441 gtagtagaaa ccaaatgcgg cattgtctgg gaacgaaaag gaagtttatt caacaaagtt
    16501 ggcgatcggc agttgtataa aaacctttcc ccggaatcta tccgcgaaga ggtagaagcc
    16561 agcttgcaac gtctgggtat tgattacatc gatatctata tgacgcactg gcagtcggtg
    16621 ccgccatttt ttacgccgat agctgaaact gtcgcagtgc ttaatgagtt aaaggctgaa
    16681 gggaaaattc gcgctatagg cgctgctaac gtcgatgctg accatatccg cgagtatctg
    16741 caatatggtg aactggatat tattcaggcg aaatacagta ttctcgaccg ggcaatggaa
    16801 aacgaactgc tgccgctatg tcgtgataat ggcattgtgg ttcaggttta ttccccgcta
    16861 gagcagggat tgttgaccgg caccatcact cgtgattacg ttccgggcgg cgctcgggca
    16921 aataaagtct ggttccagcg tgaaaacatg ctgaaagtga ttgatatgct tgaacagtgg
    16981 cagccacttt gtgctcgtta tcagtgcaca attcccactc tggcactggc gtggatatta
    17041 aaacagagtg atttaatctc cattcttagt ggggctactg caccggaaca ggtacgcgaa
    17101 aatgtcgcgg cactgaatat caacttatcg gatgcagacg caacattgat gagggaaatg
    17161 gcagaggccc tggagcgtta aatactcaac acgattcacc tctgtttctg tgaaaactgt
    17221 gatgaaaatc tgtgattcaa ataggttatt ttgaaagtaa acatcggtta tgcgataatc
    17281 gcgctaatgt gatgtaaaaa gatacagggg tgtaatcgtg gcggcaaaag acaggattca
    17341 ggcaattaag caaatggttg ccaacgataa aaaggtgaca gtctcaaatt tgagtgggat
    17401 ttttcaggta accgaagaaa ccattcgccg cgatcttgag aagctggaag atgaaggctt
    17461 tttgaccaga acctatggtg gtgctgtttt aaatacagcg gtgttgacgg agaatatcca
    17521 tttttataag cgcgcttcat cgttttatga agagaagcag cttattgcac gcaaggcact
    17581 accctttatc gacaataaaa ccaccatggc agccgattcc agtagtaccg taatggaatt
    17641 gctcaaattg ttacaggacc gtagtgacct gacgttgcta accaactcgg cggaagcgat
    17701 tcatgttctg gctcagtcag aaattaaagt cgtttcaacg ggtggggaac taaacaaaaa
    17761 tacactttcc ctgcaaggaa gaattactaa agagatcatc agccgctacc atgtcgatat
    17821 catggtaatg agttgtaaag gtcttgatat taacagcggc gcgctggact ctaatgaagc
    17881 ggaagctgaa atcaaaaaga caatgatccg tcaggcgaca gaagttgcgt tattggttga
    17941 tcactctaag tttgatcgta aagcttttgt ccagttagct gactttagtc atattaatta
    18001 tataataact gataaatcac cgggtgcaga gtggatagca ttttgcaaag acaataatat
    18061 ccagctggtg tggtaattta agcacacgtt gtcaatatta tttgttcctg cgcctggaaa
    18121 taaaatgatg ataaagatgc ggcaagtgaa tttgccgtaa ttgctacctt ttatagcaaa
    18181 tccgaggcac ccaatggaac aatatgatca aattggcgca agactggacc gcttgccttt
    18241 ggcccggttt cattatcgta tatttggtat aataagcttt agtctgttat taacggggtt
    18301 tttgagttac tcaggtaatg tcgtcttagc aaagctggta agcaatggat ggtcaaataa
    18361 tttcctcaat gccgccttta cctcggcatt aatgtttggt tatttcatcg gctcacttac
    18421 tggtgggttt attggtgact actttgggcg gcgcagggcg tttcgcataa atcttctcat
    18481 cgtcggtatt gctgcaacag gggccgcttt tgtccctgat atgtactggc tcattttctt
    18541 gcgcttcctg atgggaacag gaatgggggc gctgattatg gttggctatg cctcatttac
    18601 ggagtttatc cccgcgacgg tgcgtggaaa atggtccgcg cggctctcat ttgttggtaa
    18661 ctggtcgccc atgctgtctg cggcgatagg cgtggtggtt atcgcttttt ttagttggcg
    18721 aataatgttt cttctgggtg gtattggcat actgttagcc tggtttctct caggtaaata
    18781 ctttatcgag tcgccacgat ggctggcagg gaaagggcaa atcgccggtg cagaaagcca
    18841 acttcgtgaa gtagagcagc aaattgaaag ggagaagagt attcgtttac cccagcttac
    18901 tttgaaccag agcaacagca aggttaaggt aatcaagggt accttcaggc tcctgtttaa
    18961 aggggaaatg ttacgacgta cattagttgc gataactgtt ttaattgcaa tgaacatttc
    19021 gctttatacc attaccgtat ggataccgac catatttgtt aactccggca ttgatgtcga
    19081 taaatcaata ttaatgaccg ctgttattat gattggtgct ccggtaggaa tatttattgc
    19141 ggcattaatt attgatcatt ttcctcgtcg attatttggc tccgccttac ttattattat
    19201 tgccgtgtta ggctatatct attcaagtca gactacagag tgggcgattt taatctatgg
    19261 tctggtgatg atcttctttt tatacatgta tgtttgcttc gcgtcggcgg tttatatccc
    19321 ggagctttgg ccaacgcatt tacgcctgcg cggttcgggt ttcgttaatg ccgtcggacg
    19381 gatcgtcgca gttttcacgc cctatggcgt tgcggcatta ttaacacatt atgggtcgat
    19441 cacggtgttt atggtgcttg gtgtcatgtt agtgctctgt gcgctggttc tctccatttt
    19501 tggcatcgaa acgcggaagg tgtcgctgga agagatttct gaggtgaatt aaatctttcg
    19561 ttgatggcat caattgccag atgtaccact gacgtgtttt atttggccta caatgccagt
    19621 acagaattgt aggccagcgt aaaattaccc ctgcgtctct tcccagtctg ccagcgtata
    19681 tagcgttgcc ccagctgctg acatctccat aaacgcgtgt gcactgtcct ggggctggat
    19741 attcacgcca cgacagccat cggtaatcac gtttacctta taacctaact gtaacgcgtc
    19801 cagcacggta aacttcacgc aatagtcagt agccaggccc ataacgatca attcatcgat
    19861 ttcatgatcg cgcaaccagt catcaagcgc ggttttctgc cgacgtccgt tatcaaaaaa
    19921 ggcactgtaa ctgtcaacta aaggattttc gcctttatgg aacaccgctg cgatctcttt
    19981 ttgattcagt aacggatgta attgtgcgcc ttcactgttc tgcacacagt gttcaggcca
    20041 gaaagtttgc cgcaggccgt cgagttggcc tggcgtataa ggctctacgc cgtgctggct
    20101 ggcaaagctg ccgtgattcg ccggatgcca gtcctggctg gcgataaccg cttctccgcg
    20161 cgactggcac cagtcaataa ggcggttagc aacctccacc gtactgtcac cttccggtac
    20221 ggcgagcgcg ccaccagcac agaaatcatt ttgtaaatcg accagtaaca gggcgcgatg
    20281 gggcattgca gtctccttaa tcatccggcg tcagttcgcc gcgcaggttt tggctcatgg
    20341 ccttgcgaat ggtttcagta tccagttcct ggctcagcag gtaatgcagt ttggttagcg
    20401 tggcttctac agtcatatct gcaccgccaa taacgccggc atgggcgagg acgttaccgg
    20461 tggcataacc actcatattc actttaccgg acatacattg tgtcaggttg accaccacaa
    20521 taccgcgatc gctggcttct tgtaattcct gcaggaaggc tttgttttgt ggcgcattac
    20581 ccacgccata ggagcgcaga atcaatgctt tcaccggttg gcgcagaaaa ttgcgcacga
    20641 cgtcagcaga aatccctgga taaatcgtca ctacgccaat tggttgtggc gtgattggat
    20701 gaacgattaa ttccccttca ccgtgcgggg cgggtggcgt attcaaacga cgaatatgga
    20761 taccagcttc cagtaacgga ggaaggtttg gagaggcaaa cgcatcaaaa ccatcggcat
    20821 gggctttggt agtgcggttg ccgcgataca atcggttatt gaaaaagagc gttacttcgt
    20881 tgatcggata attcgccgca acgtacaacg cattcagcag attaatttgt ccgtcagagc
    20941 gtaactcagc cagcgggatt tgtgaccctg tcacaatgac cggtttaccg agattctcga
    21001 gcatgaacga cagcgcagag gcggtatacg ccatcgtgtc ggtaccgtgt agaatgacaa
    21061 aaccatcata gtcgtcatag tgcgctttaa tatcttcagc aatatgctgc cagtcttccg
    21121 gcgtcatatc agaagaatcc atcaacggcg tatattcatg aatggtgaaa tctggcatct
    21181 ccaggcgatg gaattccggc atcagcgcca gttggcgttg tagatgacct gacaccggaa
    21241 tataaccctg ctcggaacgc tgcatcccga tggtcccgcc cgtgtaggcg acgtaaattg
    21301 atttcttttg catgatattg atatgaatga tatcgaaaga gcaaaagtat atacgtgagg
    21361 caggaggagc gcacctgata ctatgcggat aaggaaatat ggggcgagac agcgggaaga
    21421 ggattcgtcg aggtaaaaaa taatgtggcc gacgatctgt cggccactca gtacaagact
    21481 taacgcacgt tggcgcaggt caggcaaaac gcataacggt tttgtgggtc gttaaacgcg
    21541 gccagcttgt cgctttcact tttaacggta gaggctaccg aggcaagcgg tgcaggtaac
    21601 atggcctgga acgcatctgg caacattgcc cggacagaac cagacatgtt gtccatcact
    21661 ttgtcgaaga aggtcggttc atcaacgtag tattccagat gccactgttt cacttttgcg
    21721 agctctgctg ctttggcaac cgcatcatcg aaatccccga gactatcgac cagcccgtta
    21781 gcttttgcat cctgaccggt ccagacgtgg ccctgggcga ttttatcaat ttgctccggc
    21841 gtcgaatgac gcgcatcagc aaccagcgtg ataaagcgtt tatagccatt ctcaatgctt
    21901 aattgcatca tctgctgcgc ttccggcggc agtgccctgg tgatagaaac atccgccagc
    21961 ggtgaagttg agacaccatc agtatgaaca ccaatcgaat ccagactatt ttctacggtg
    22021 gtgatcacgc cgaagatacc gatagaaccg gtcagggtgc tggggttagc cacaatgtaa
    22081 ttagctggcg tggaaatcca gtaaccacca gatgccgcca tgccgcccat cgatacaacc
    22141 acagacttac ccgctgcccg ggctgctgcc agttcagcgc gaatcacttc agacgcggta
    22201 acgctgccgc ctgggctatt aacacgcagg acaatcgctt tcactttcgg gtcaaggcga
    22261 gcgtcgcgga tttgtgccgc agtggtatca ccgccaacat tcccctgagt ttcctcgcca
    22321 tccataattg cgccattagc gaagacgaca ccgatgctgt caccggtatc tgccggcgtt
    22381 ttcaatgcgt aatcgtaata actgatggcg cgataatttt tatcagtctt actccagccg
    22441 aactctttgg tcagagcttt ttcgatttcg gcactcgatg ccagtgcatc gaccagcttg
    22501 ttttccagtg catatttcgc ggtatcgcca ccggttttgg ttaaaccctc aagcaaccct
    22561 tgcgcgccag ggaatacctg ctgagcaggg atctgccggt tagcggcaac agtattcaga
    22621 tagttttgcc acagctcacc aatccagcgg ctgtcagctt cgcgggctgc cggtgacata
    22681 tcatcacgaa taaacggttc aacggcagat ttatacgtac ccacgcggaa cacatgggtg
    22741 gaaactttca gcttatccag caacgatttg tagtacagac cgttggtagc aaaaccgtgc
    22801 agatcaacca cgccttgcgg tgacagccaa attttattgg cgaaactggc gagataatat
    22861 tgcccctggc tgtagttctc gccaacggca taaaccggtt tcccgctgtc acgaaactct
    22921 ttcagggctt tgccgatgta ctgcatagac ggctggtcgc cgcctgcgaa gtttttcaga
    22981 tccatcacga taccggtgat attgcggtcg tccttcgcct ggcgaatggt gttgacgata
    23041 tcaaacagtg agttttcctg cagacgatcg gaactggcac caagcagctg gcggcttaat
    23101 ttactaaaac gctgagaact gtctggttta tcgacgatca caccagaaat gtccagcagc
    23161 agtgcgccac gactggccgt ttctttcgaa tcaccaccac tgacctgcat ccagatcccc
    23221 acaccaacca gtacgaggaa aataaagaac aggttaagta ccatttcacg gacgaaattc
    23281 agcagacgcc acgtccattt aaaaaatccg gcaataaatc gccaaagggt tcgcatgtat
    23341 tctcccaact taaggtcaca cctgtcacag gcgcaataga tgtggttatc gtaatgaccc
    23401 aaccggcact tgtcagcagg aattgcggac tgtgctgtaa caaaatccac tctcgtgtta
    23461 attttgtgaa taaatatcac gacaggagtt aatcaaatgg atgcactcga actattgatc
    23521 aatcgccgta gcgcctcccg cttggctgaa cccgcgccaa cgggtgaaca actgcaaaac
    23581 atcctgcgtg cgggtatgcg tgcgccggac cataagtcca tgcaaccgtg gcattttttt
    23641 gtgattgaag gggaagggtg cgagcgtttc agcgccgtac tggaacaggg ggcgattgct
    23701 gccggtagtg atgataaagc tatcgacaaa gcccgtaatg cgccgttccg cgcaccgctc
    23761 atcatcacgg tggtggcgaa atgcaaagaa aatcataaag tcccgcgatg ggaacaggaa
    23821 atgtctgccg gatgcgcggt catggcgatg caaatggcag ccgttgccca gggatttggc
    23881 ggcatctggc gcagtggcgc attaactgaa agtccggtag tgcgtgaagc attcggttgc
    23941 cgtgagcagg ataaaattgt cggttttctc tacctcggca cgccgcagtt gaaagcatct
    24001 acgtcgatta acgtcccgga cccgacgccg tttgtaactt atttctgata attgtcgcga
    24061 aactgtctgg attgtgagca aaggcgcagg aattcagaca ctctcactta tcacttcacg
    24121 gaatgagggt taccataccc ggattgcatt gacaggagat gtccatgagc gagaactcga
    24181 ttcgtttgac ccaatacagc cacggagctg gttgcggctg taaaatttcc ccaaaagtgt
    24241 tggaaaccat cctgcacagt gagcaggcga agtttgttga tccgaatttg cttgtgggta
    24301 atgaaacccg cgacgatgcg gcggtgtacg atctgggcaa tggcaccagc gttatcagta
    24361 ccaccgactt ctttatgccg atcgttgata atcctttcga ttttggccgc attgcggcga
    24421 ctaacgccat cagcgatatc ttcgcgatgg ggggcaaacc gattatggcg attgcgatcc
    24481 tcggctggcc gattaacaaa ctttccccgg aaattgcccg cgaagtaacc gaaggtggac
    24541 gctatgcatg ccgtcaggcg ggtattgcgc tggctggcgg tcactccatc gatgcgccgg
    24601 agccgatttt tggtctggcg gtaacgggga tcgtaccgac cgagcgggtg aagaaaaaca
    24661 gtaccgcaca agccggatgc aaactgttcc tgacgaaacc gctggggatc ggcgttctta
    24721 ccacggctga gaaaaaatca ctgttgaaac cagaacatca gggactggcg acggaagtga
    24781 tgtgccggat gaacatcgca ggcgcgtcct ttgccaacat cgaaggcgta aaagcgatga
    24841 ccgacgttac gggctttggt ctgctgggcc acttgagcga aatgtgtcag ggggctggtg
    24901 tgcaggcacg cgtcgactat gaagcgatcc cgaaactccc cggtgttgaa gagtacatta
    24961 agttgggcgc agtacctggc ggcactgaac gtaactttgc cagctacggt catctgatgg
    25021 gtgaaatgcc gcgtgaagtg cgcgatctgc tgtgtgatcc gcaaacttct ggcggtttgc
    25081 tgctggcggt catgccggaa gcagaaaatg aggttaaaac tacagccgcc gagtttggca
    25141 ttgaactgac ggcgattggc gaactggtgc cagcgcgcgg cggtcgtgcc atggttgaga
    25201 tccgttaatt caatgcggtt gtttattgcc gaaaaaccga gtctggcgcg cgccattgct
    25261 gatgtcctgc ccaaaccgca ccggaaaggc gatggcttta tcgagtgcgg taatggtcag
    25321 gtggtgactt ggtgtatcgg tcacctgctt gagcaggcgc agccagacgc ctacgacagc
    25381 cgctatgcgc gctggaatct tgcggatttg ccgattgtcc cggaaaagtg gcaattacag
    25441 ccacgaccct ccgtgaccaa acaacttaac gtcatcaaac gcttcctgca tgaagccagc
    25501 gaaatcgttc acgccgggga cccggatcgt gaagggcaat tgctggtgga tgaagtgctg
    25561 gactatctgc aactggcacc ggaaaagcgc cagcaggtgc agcgttgctt gataaacgac
    25621 ctgaacccgc aggcggttga gcgggcgatc gaccgtcttc gttccaacag tgagtttgtg
    25681 ccattgtgcg tttctgcgct ggcgcgagcg cgtgccgact ggctgtacgg catcaatatg
    25741 acccgtgcgt ataccattct cggtcgcaat gccggttatc agggcgtact ttccgtggga
    25801 cgcgtgcaga cacccgtgct cgggctggtg gtgcgccgcg atgaagagat tgaaaacttc
    25861 gtggcgaaag acttctttga agtcaaagca catatcgtga cacctgccga tgagcggttt
    25921 accgctatct ggcaaccgag cgaagcgtgt gaaccgtacc aggatgaaga agggcgcttg
    25981 ttacatcgtc cactggcgga gcatgtggtt aaccgcatta gtggtcaacc ggctattgtc
    26041 accagctata acgataaacg ggaatcagaa tccgcgccgc tgccgttttc gctttcggcg
    26101 ttgcagattg aagcggcaaa acgcttcggt ctgagcgcgc agaacgtgct tgatatctgc
    26161 cagaagctgt acgaaacgca caagctaatc acttatccgc gttccgattg tcgctatttg
    26221 ccagaagaac attttgccgg acgccacgcg gtgatgaatg ccatcagcgt tcatgcaccg
    26281 gatctgttgc cgcagccagt ggtagatcca gatatacgca accgctgttg ggatgacaaa
    26341 aaggtcgatg cgcaccacgc catcatcccg accgcacgga gttctgcgat caacctgacg
    26401 gagaacgaag cgaaggtcta taacctgatt gcccgtcaat acctgatgca gttctgcccg
    26461 gatgcggtgt tccgcaagtg tgttatcgaa caggacattg ccaaaggcaa atttgtcgct
    26521 aaagcccgtt ttcttgctga agcaggctgg cgcacgctgt taggcagcaa agagcgcgat
    26581 gaagaaaacg acggcacgcc attgcctgtg gtggcgaaag gcgatgagtt gctgtgtgaa
    26641 aaaggtgaag tggtagagcg gcaaacccag ccgccgcgcc attttaccga tgcaacactg
    26701 ctttcggcga tgaccgggat cgcgcgcttt gtgcaggaca aagatctgaa aaaggtcctt
    26761 cgtgcgaccg atggtctggg gacagaagca acccgtgccg ggattattga actgctgttc
    26821 aagcgtggtt ttctgaccaa aaaagggcgc tatatccact ccaccgacgc cggaaaagcg
    26881 ttattccatt cgctgccaga aatggcgacg cgaccggaca tgaccgcgca ctgggaatcg
    26941 gtgctgacgc aaatcagcga aaagcagtgt cgctatcagg actttatgca gccgctggtg
    27001 gggacgctat atcagcttat tgatcaagcc aaacgtacgc cggtgcggca gtttcgcggc
    27061 attgtggctc cgggcagtgg tggcagtgct gataagaaaa aggctgcacc gcgtaaacgt
    27121 agtgcgaaaa aaagtccgcc agcagatgaa gccggaagcg tggcgatagc gtaagcgagc
    27181 gaatctttcg tgctattcga gtcatattct gaaatatcca gcggatcaag aaaattcgtt
    27241 ggatattttt ttgcatggat aaaattatcg cctctaaagt atgtaataac agggaatgtg
    27301 atgaaaaaag ttttattaca aaaccatcct gggagcgaga agtattcttt taatggctgg
    27361 ggaatattta atagtaattt tgaacgggtg attaaagaaa ataaggccat gctgctttgt
    27421 aagtgggggt tttatttaac atgtgttgtc gctgtaatgt ttgtattcgc agcgataaca
    27481 tccaacggtt tgaatgaaag aggcctgatt accgcgggat gctcttttct ttatctatta
    27541 attatgatgg ggcttattgt tcgggccggt tttaaagcaa aaaaagaaca actacattat
    27601 tatcaggcta aaggtattga gccgctcagt atcgagaagt tacaggcgct acaattgatc
    27661 gcaccttatc gattctatca taagcaatgg tctgaaacgc tagagttctg gccgcgaaag
    27721 cctgaacctg gcaaagatac cttccaatat catgtgcttc cttttgactc gatcgatatc
    27781 atcagtaaaa gacgcgagtc tttagagaat caatggggta tcgaagatag cgaaagttat
    27841 tgtgccttaa tggagcattt tctttctggc gaccatggag ccaatacctt taaagcaaac
    27901 atggaggaag ccccagagca ggttatcgcc gtgttgaata aatttgctgt ttttccctca
    27961 gactatatct ctgattgcgc taataatcgc tccggtaaat cctcggcgaa gctaatatgg
    28021 gcggcggaat tatcatggat gatctcgata tcaagcacag cttttcaaaa cgggacaatt
    28081 gaagaagaac tggcctggca ttatataatg cttgcttctc gaaaggcgca cgagttgttc
    28141 gaaagcgaag aagattatca aaaaaatagt ctaatgggat ttctttactg gcatatctgc
    28201 tgctatcgca gaaagttaac ggatgctgaa cttgaagcgt gttatcgtta cgacaagcag
    28261 ttttgggagc actacagtaa gaaatgccgt tggcccataa gaaatgttcc ctggggagca
    28321 tcatccgtta aatactcata aacgcctgaa attttgccgg gggcgctgcg cttgcccggc
    28381 ctacattaga gcgtaactgc aatgaattgt gcccatctgt aggcctgata agcgtagcgc
    28441 catcaggcat ttacaactta aatcacaccc tgcgccagca tcgcatcggc aaccttcaca
    28501 aaaccggcaa tgttcgcgcc ctgcacgtag ttggtttgct caccttcacc accatgctca
    28561 acacaggcat ggtggatatc cagcatgatg tgatgcaaac gtgcgtcaac tttctcggct
    28621 ttccagccca ggcgcgcagc gttttgtgcc atttccaggc ccgacgttgc tacaccgcca
    28681 gcattagccg ctttacccgg tgcaaacagt acgcctgcct gctggaacag ttcagtcgct
    28741 tcgatggtgg tcggcatatt tgccccttca gcgacggctt taacgccatt ggcgataagc
    28801 tgatgcgcgg tgtcaacatc cagttcgttc tgggtggcgc aaggcagggc gatatcaacc
    28861 ggtacagacc acggctgttg gccttcgaga tagaccagac caaattcttt ggcgtaatct
    28921 gccactcgac catcgcggct ggctttgatt tcgataagac gtgccagttt ctctttcgtg
    28981 aatccgcttt catcaactac agtgccgctg gagtctgacg cagtgatcgc acgagcacca
    29041 aattccatcg ctttttcgat agcgtactgg gcgacgttgc cggagccaga aacggaaacg
    29101 cgcatccctt caaaacccat accgtggcgt tttaacattg cttctgtgaa ataaaccaga
    29161 ccgtagccgg tagcttccgg gcgaataaga ctaccgccaa atgaaaggcc cttaccggtg
    29221 aagacgcagg cggtattgtt ggagagcttt ttcatcatcc ccgccataaa gccgacttca
    29281 cgaccaccaa cgccgatatc acctgccgga acgtcggtat ccgcgcccag gtggcgatac
    29341 agttcagtca tcagcgcctg gcaaaaacgc atcactttac cttcgctttt tcctttcgga
    29401 tcgaaatcgc tgccgccttt accaccgccc atcggcaggg tagtcagggc atttttgaag
    29461 gtttgttcaa agccgaggaa tttgagaatg gaaaggttaa ctgacggatg gaagcgcata
    29521 ccgcctttgt acgggccgat ggcagagctg aactgcacac gccatgcacg gttgacctgt
    29581 atctggttgc gatcatcaac ccataccacg cgaaactgga tcacgcgctc cggttcaacc
    29641 agacgctcca gtaatgacat ctggcgatat tttggatttt gttcaagaaa aggccagagt
    29701 gtggtcatta cttcacgaac ggcttgcgcg aactcggttt gattcgggtc gcgcttttgg
    29761 acatggttga ggaatgactc cagagaatat gtctgat
//
LOCUS       DADMEQ010000040        29652 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.040, whole genome shotgun sequence.
ACCESSION   DADMEQ010000040 DADMEQ010000000
VERSION     DADMEQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.040"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            347..640
                     /locus_tag="JNZ05_001810"
     CDS             347..640
                     /locus_tag="JNZ05_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565069.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            712..1371
                     /locus_tag="JNZ05_001811"
     CDS             712..1371
                     /locus_tag="JNZ05_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAY8565070.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1448..1909
                     /locus_tag="JNZ05_001812"
     CDS             1448..1909
                     /locus_tag="JNZ05_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415699.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HAY8565071.1"
                     /translation="MAEHLMSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDED
                     TDEIYFTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLA
                     EGKDLPAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="JNZ05_001813"
                     /pseudo
     CDS             complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="JNZ05_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3389..3808
                     /gene="umuD"
                     /locus_tag="JNZ05_001814"
     CDS             3389..3808
                     /gene="umuD"
                     /locus_tag="JNZ05_001814"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAY8565072.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3808..5075
                     /locus_tag="JNZ05_001815"
                     /pseudo
     CDS             3808..5075
                     /locus_tag="JNZ05_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            complement(5121..5651)
                     /gene="dsbB"
                     /locus_tag="JNZ05_001816"
     CDS             complement(5121..5651)
                     /gene="dsbB"
                     /locus_tag="JNZ05_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HAY8565073.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5797..7338)
                     /gene="nhaB"
                     /locus_tag="JNZ05_001817"
     CDS             complement(5797..7338)
                     /gene="nhaB"
                     /locus_tag="JNZ05_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HAY8565074.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREGVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7559..8278
                     /gene="fadR"
                     /locus_tag="JNZ05_001818"
     CDS             7559..8278
                     /gene="fadR"
                     /locus_tag="JNZ05_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HAY8565075.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8330..9862)
                     /locus_tag="JNZ05_001819"
     CDS             complement(8330..9862)
                     /locus_tag="JNZ05_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HAY8565076.1"
                     /translation="MATIDSINKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARHYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10192..11490
                     /locus_tag="JNZ05_001820"
     CDS             10192..11490
                     /locus_tag="JNZ05_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HAY8565077.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11500..12570
                     /gene="dadX"
                     /locus_tag="JNZ05_001821"
     CDS             11500..12570
                     /gene="dadX"
                     /locus_tag="JNZ05_001821"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HAY8565078.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12738..14164)
                     /locus_tag="JNZ05_001822"
                     /pseudo
     CDS             complement(12738..14164)
                     /locus_tag="JNZ05_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109184.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14774..16510)
                     /locus_tag="JNZ05_001823"
     CDS             complement(14774..16510)
                     /locus_tag="JNZ05_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HAY8565079.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYS
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16605..17519)
                     /gene="ldcA"
                     /locus_tag="JNZ05_001824"
     CDS             complement(16605..17519)
                     /gene="ldcA"
                     /locus_tag="JNZ05_001824"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HAY8565080.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            17619..18230
                     /gene="emtA"
                     /locus_tag="JNZ05_001825"
     CDS             17619..18230
                     /gene="emtA"
                     /locus_tag="JNZ05_001825"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HAY8565081.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLRYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18232..18966)
                     /locus_tag="JNZ05_001826"
     CDS             complement(18232..18966)
                     /locus_tag="JNZ05_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HAY8565082.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19167..19340
                     /locus_tag="JNZ05_001827"
     CDS             19167..19340
                     /locus_tag="JNZ05_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565083.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGA
                     ILVLGVFRLLRRE"
     gene            complement(19390..20850)
                     /locus_tag="JNZ05_001828"
     CDS             complement(19390..20850)
                     /locus_tag="JNZ05_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY8565084.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNHIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20787..21344)
                     /locus_tag="JNZ05_001829"
     CDS             complement(20787..21344)
                     /locus_tag="JNZ05_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HAY8565085.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARVLTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21370..22224)
                     /locus_tag="JNZ05_001830"
     CDS             complement(21370..22224)
                     /locus_tag="JNZ05_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HAY8565086.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22224..23033)
                     /locus_tag="JNZ05_001831"
     CDS             complement(22224..23033)
                     /locus_tag="JNZ05_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAY8565087.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23043..23801)
                     /locus_tag="JNZ05_001832"
     CDS             complement(23043..23801)
                     /locus_tag="JNZ05_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8565088.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APIIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23798..24778)
                     /locus_tag="JNZ05_001833"
     CDS             complement(23798..24778)
                     /locus_tag="JNZ05_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAY8565089.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVSV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGELMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24778..25800)
                     /locus_tag="JNZ05_001834"
     CDS             complement(24778..25800)
                     /locus_tag="JNZ05_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HAY8565090.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26866..27306
                     /locus_tag="JNZ05_001835"
     CDS             26866..27306
                     /locus_tag="JNZ05_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565091.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(27385..29082)
                     /locus_tag="JNZ05_001836"
     CDS             complement(27385..29082)
                     /locus_tag="JNZ05_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HAY8565092.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(29402..>29652)
                     /locus_tag="JNZ05_001837"
     CDS             complement(29402..>29652)
                     /locus_tag="JNZ05_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="phosphotransferaese subunit; phosphorylates
                     dihydroxyacetone along with DhaK/DhaL; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAY8565093.1"
                     /translation="PQFNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALI
                     ARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG"
BASE COUNT         7449 a         7204 c         7609 g         7390 t
ORIGIN      
        1 atggcctttg ctgaactcaa cattgacatt cttacccgca gcaaagctcg tggaggttag
       61 cagtgcaagc agcaatacag ccttactgat gctcttgatt ttcattggta gtctcctgtt
      121 cagaatgagt acgatgaact gttataatat aacaatccct tacgggagaa ttccagtggc
      181 ttttctgcgt tcggtaaaat cccggcgcgg cattttttgc cggggggatt acttataaag
      241 gggcatggac tcaacaaacc gcagaaatct acagctggct ggcttgtggc gcgggtttca
      301 ttgatgttag cttatgccaa aacctggcat cttaaagagt aaaagtatgt tttgtgtgat
      361 ttatcgaagc agcaagcgtg accagaccta tttatatgtc gaaaaaaaag acgatttttc
      421 gcgtgttcct gaggaactga tgaaaggttt tggtcagcct cagttagcga tgattctgcc
      481 gttggatggg cggaagaaac tggtcaatgc tgatattgaa aaggttaaac tggcattaac
      541 cgagcaaggt tactatttgc agttaccgcc accacccgaa gatttgctga agcaacatct
      601 ttccgtcatg gggcagaaaa cagacgacac taacaaataa ccgatatccg gcggtggcat
      661 tatctttgtc ggcgcgggtt ttcatatcca cgataaggtg aggggaacgt tatgtatcaa
      721 catcacaact ggcaaggtgc gctgctggat tatccggtga gtaaagtagt ctgtgttggc
      781 agtaactatg ccaaacatat taaagagatg ggcagcgcag tgcccgaaga gccagtgctg
      841 tttattaaac cagaaacggc actgtgcgat ctgcggcagc cattggcgat cccatccgat
      901 ttcggttcag ttcatcatga agtcgaactg gcggtgttga ttggcgcgac gctgcgtcag
      961 gcaacggaag agcatgtccg caaagccatt gccggttacg gcgtggcgct tgatctgaca
     1021 ttgcgtgatg ttcagggaaa aatgaagaaa gccgggcagc cgtgggaaaa ggctaaagcg
     1081 tttgataact cttgtccgct ttccgggttt atccccgcgg cggaatttac cggcgatcca
     1141 caaaatacaa cgctgggcct gagcgtaaac ggcgaaaaac gccagcaagg tacgactgcg
     1201 gacatgatcc ataaaatcgt tccgttgatc gcttatatga gcaagttttt taccctcaag
     1261 gccggtgacg ttgtgttgac aggcacgcct gatggcgtcg gcccgttgca aagcggtgat
     1321 gagctgacag tcactttcga tgggcattct ttgacaactc gcgttttgta atactttttg
     1381 ccgccagaaa gcggcggcaa aacttgcatc gctgtgccag actggttata aggtgcgttt
     1441 taacgtaatg gcggaacacc tgatgagcga tgtacctttc tggcaaagta aaaccctgga
     1501 cgaaatgagc gatgcggaat gggagtcgtt gtgtgatggt tgcggtcagt gttgcctgca
     1561 taaactgatg gatgaagaca ccgacgaaat ctacttcact aacgtcgcct gtcgccagct
     1621 caatattaaa acctgtcaat gtcggaacta cgaacgtcgt ttcgagtttg aacccgactg
     1681 cattaaatta acccgtgaaa atctgccaac attcgaatgg ctgccaatga cctgcgctta
     1741 tcggttgctg gcggaaggta aagatttacc tgcgtggcat ccgctactta ctggttcgaa
     1801 agcggcaatg catggtgaac gtatctctgt gcgtcatatc gcagtgaaag aatcagaagt
     1861 gattgactgg caggatcata tcttaaataa acctgactgg gcgcagtgat aaatttaata
     1921 gatgctgatg ggggctttct taatatacgt ctggatgctc gccatttcgt agccctgttt
     1981 ttgaaagcgc tctgtcataa tgagagttac ccgatacctt attatcaaca ggtaactctc
     2041 aatctttaaa aaacgtctgt gtttttaaat gtgataaacc ttataccttg agtacatgga
     2101 gagaataatc gcttatcaga cttcaggtac ttcaaagagt gtctttttac cgtgtctttt
     2161 ctgatactca ttacaggtgt taatcatttt tttggccgct tcttttagca aagaaagcat
     2221 taaatcatca taatcaacat agaatctggt tgtttcagtt tccgttttta tctccccgat
     2281 ggcggctatt tcggtggtta atttcaattt ggcggcatcg atatctttat tcgcttgttt
     2341 aaccgtgtta gacagggtgg taaagaaact ctgcacagat tttaacttgt tcttcaattc
     2401 tggaatcagt ttcccttcaa ctacgcccgc agcaatagaa taggaaatga ttaaaccaaa
     2461 tggaccggcg acgacatcgg ctgcggcacc ggcatacgct tccttcctga ttttatctac
     2521 ctgtgactgg aaatagctgc ttttttctga aaaatcattg gttaactggc tatctaacgc
     2581 cagcagtttc ccggaagcgt tgttgaaact ttgtgagctt accagcaggg atttttgcgc
     2641 ttcattcagc ttcgtgatgc cgtcatccag taccttaatg aggggcggat gctttcttct
     2701 cattgtactc atcaaatagc aaaatatacg ctgcgagcaa ttgcgtcgca acaccacacc
     2761 attcatacac tgtttgggtt gcttcaaaat acttatcctg gctatccata agtaaggttt
     2821 taatatcgcc gactaaaacg gaggctgcct gtgaatactc ctgtttaaag cgacttaact
     2881 cttttatggt ttcatcaaag gtctgccagg ggatgacctg atcgagatat ttattataaa
     2941 gatctaatgc tccatctgcg gtttcgattg cgtttttaac tacttctacc gttttatctg
     3001 caacgatttc agtcataatc attcgcctct ttaaatatat aaattgtaat gaaactcctg
     3061 ttttacaact attaataaat tttacttcat ctaattcata gttagccggg cgggatgcgt
     3121 caatgtcttt atttctatta atatgataaa tatcaaacaa tgtttaatgt cattatggcg
     3181 aatgcttcta ttctattttt ttgccgggtg atatttttca tttctgctgg atgagcgtcg
     3241 tcgccagaag gccacgtgag cacaggataa gagaacgaaa aatcagcagt ctatgcagcg
     3301 acaaatattg atagcctgaa tcagtattga tctgctggca agaacagact actgtatata
     3361 aaaacagtat agcttcaggc agattattat gttgtttatc aagcctgcgg atctccgcga
     3421 aattgtgact tttccgctat ttagcgatct tgttcagtgt ggctttcctt caccggcagc
     3481 agattacgtt gaacagcgca tcgatctgaa tcaactgttg atccagcatc ccagcgcgac
     3541 ttacttcgtc aaagcaagtg gtgattctat gattgatggt ggaattagtg acggtgattt
     3601 actgattgtc gatagcgcta ttaccgccag ccatggtgat attgtcatcg ctgctgttga
     3661 cggcgagttt acggtgaaaa aattgcaact acgcccgacg gtacagctta ttcccatgaa
     3721 cagtgcgtac tcgcccatta ccatcagtag cgaagatacg ctggatgtct ttggtgtggt
     3781 gatccacgtc gttaaggcga tgcgctgatg tttgccctct gtgatgtaaa cgcgttttat
     3841 gccagctgtg agacggtgtt tcgccctgat ttatggggta aaccggtggt tgtgctatcg
     3901 aataatgacg gttgcgttat cgcccgaaac gctgaggcaa aggcgctggg cgttaaaatg
     3961 ggcgatccct ggttcaaaca aaaagatctg tttcgtcgct gtggtgtggt ttgctttagc
     4021 agcaattatg agctttacgc agacatgagc aatcgggtga tgtcgacgct ggaagaacta
     4081 tcgccccgcg tcgagattta cagtattgat gaagcattct gcgatctgac aggtgtgcgt
     4141 aattgtcgcg atctgactga ttttggcaga gaaattcgcg caacggtgct acaacgtacc
     4201 catcttactg ttggtgtggg gatcgcccag accaaaacgt tggctaagct tgccaatcat
     4261 gcggcaaaaa aatggcagcg gcagacgggt ggggtggtgg atttatcaaa tctggaacgc
     4321 cagcgtaaat taatgtctgc tctccccgtg gatgacgtct gggggattgg acggcggatc
     4381 agcaaaaaac tggacgcgat ggggatcaaa accgttctcg atttggcgga tacagatatc
     4441 cggtttatcc gtaaacattt taatgtcatg ctcgaaagaa cggtgcgtga actgcgcggc
     4501 gaaccctgtt tcaactggaa gagtttgcac cgacgaagca ggaaattatc tgttcccgct
     4561 cgtttggtga acgcatcacg gattatacgt cgatgcggca ggccatttgt agttacgctg
     4621 cccgggcggc ggaaaaactt cgcagtgagc atcaatattg tcggtttatc tccacattta
     4681 ttaagacgtc accatttgcg ctcaatgaac cttattacgg caatagcacg tcggtaaaac
     4741 tgctgacgcc cactcaggac agcagggata tcattaacgc tgctacgcga tctctggatg
     4801 ccatctggca agcgggccat cgctaccaaa aagcgggcgt gatgctgggg gatttcttca
     4861 gtcagggagt cgcgcagctc aatttattcg atgacaatgc accgcgcccc gggagtgagc
     4921 aattgatggc ggtaatggat actctgaatg ctaaagaggg cagaggaaca ctctattttg
     4981 ccgggcaggg gatccaacag caatggcaga tgaagcgagc tatgctttca ccacgttata
     5041 caacgcgaag ttctgattta ctgcgggtca aataaatata gcggcaggaa aaaagcgttc
     5101 ccgcaggagc gccgaatgga ttagcgaccg aacagatcac gttttttcgc tttaaacggc
     5161 tgggaaatca ccaccagcac cgcgacaatc aggtaagcga taaaaatacc gagcagccac
     5221 tgcggcattt ccatacctaa aaaatcccac tgacgctcgg cgcaatcgcc agaggcgaca
     5281 aacacttgcg gcacccattt atccagcggc agccattccg ggaaacgaac cataaaatca
     5341 caggtggcaa atggcgaagg atagagctga agcatggtgt gctcgtaagt taactgcaca
     5401 ccgcggaacg cactatacaa ccagataacc atcgctacat aacgcagcgg agttttcggg
     5461 gcgatcgcgc caatcagcgc agcacccaga acgccgaata acgcgcagcg ttcataaata
     5521 cagagcacgc aaggtttcag taacatcaca tgctggaacc acagcgccgt cagttccagt
     5581 gccagagcag taaacgccat caacagccac gcgccccggc cttgtgaaca ttggttcaaa
     5641 aatcgcaaca taatcatttc cctgcaatat gcatagagtg cgcagtttaa accaattcat
     5701 tcgctgcgcc accaggaggc agcaaaaata ggacgcaatt ggacaattat cgggcgaaaa
     5761 ggcaaaccgg gcatttatat gcccggtaag ttgttatcaa agcgttgcta tccagcccat
     5821 ttgcataaac cattcggtta caggggcaag cgtaaactcg acgcagagca agccgacgag
     5881 tgtcagtacg agggtgtaag gcagggccat ccacaccatg cggccataag agaggcgaat
     5941 caatggcgcg agtgcagagg tcagcaggaa caggaacgca gcctgaccgt tcggcgtagc
     6001 gacagagggc agattggtac cggtattaat ggcgaccgcc agcagctcgt attgcttcaa
     6061 cgtgatagcg ccactttcca ttgccgcttt cgcttcgttg atataaatcg tccccacgaa
     6121 gacgttatcc gaaatggatg acagcagacc gttgaaaata tagaacagcg acagctgagc
     6181 atgttcagat gcctgcaaca caaactgaat aatcggcgaa aacagttgtt ggtcgataat
     6241 caccgcgact accgagaaaa acaccgtcaa cagtgcggtg aatggcagag attcggtgaa
     6301 ggctttaccg atagcatgtt catcggtgac accggtcaat gatgttgcca gaataatgac
     6361 tgacaaacca atcaagccaa cttccgccag atgcaacgcc agcgcagtca ccagccagac
     6421 gccaataatc gcctggacaa tcagacggat tttatcctga cgggtacgct ggtggcggct
     6481 ttgatcgtca aactgttgca acacttcgcg gactttctcc ggcagcgttt caccgtagcc
     6541 aaaccaacgc agcttctcta ccagcaggca ggttaacagg ccacaaatca gaaccggaac
     6601 ggtcaccggc gacatgcgca ggaagaaatc gccaaaatgc cagccagccg ctttagcgat
     6661 gatcaggttc tgtggttcgc ccaccatggt cattacgccg cctaatgcgg tgccgacacc
     6721 ggcatgcatc atcaggctac gcagaaagcc acggaactgt tccagaacca cgttgtaatg
     6781 cttgtcgata tgactatcgt cttgcaggtc ggtgtcttcg gtacgggaag aggctacgcg
     6841 atgataaata ccataaaaac cgactgcaac gctgatcacc accgccacga cggttaaggc
     6901 atcgaggaac gcggagagga acgctgccgc cacgcaaaaa gagagcgaca gcagcatttt
     6961 ggagcgaatg cttaacagca aacgggtaaa tatgaacagc aacagctgtt tcataaaata
     7021 gatacccgcc accataaaca tcaataacag caagacttca agatttgccg ccaccccttc
     7081 acggacgtgt tccgcgctgg tcatgccgat gaataccgct tcgatagcca acagaccacc
     7141 ggggagtagc gggtagcatt tcagggccat cgccagagtg aaaataaatt ccgctaccag
     7201 caaccagccc gcgacgaaag ggctgatgag gaaaattaac gggtttacga ttaagaaaat
     7261 gatgagggcg agtttgtacc agtcggggga ctggcccaaa aaattgcgcc atagcgcgcg
     7321 gccccaggag atctccatga tggtttccct taccttacaa ataatcaatg atgtttttgt
     7381 gtttaaacgc aaagcttaac ggtcaggcag gagtgaggca agtcttgata gtcaagggga
     7441 aagagatgcg gaaaatgaag ccttgatccc tttttcttct ttttgtctgc tatcagcgta
     7501 gttagccctc tggtatgatg agtccaactt tgttttgctg tgttatggaa atctcactat
     7561 ggtcattaag gcgcaaagcc cggcgggttt cgcggaagag tacattattg aaagtatctg
     7621 gaataaccgc ttccctcccg ggactatttt gcccgcagaa cgtgaacttt cagaattaat
     7681 tggcgtaacg cgtactacgt tacgtgaagt gttacagcgt ctggcacgag atggctggtt
     7741 gaccattcaa catggcaagc cgacgaaggt gaataatttc tgggaaactt ccggtttaaa
     7801 tatccttgaa acactggcgc gactggatca cgaaagtgtg ccgcagctta ttgataattt
     7861 gctgtcggtg cgtaccaata tttccactat ttttattcgc accgcgtttc gtcagcatcc
     7921 cgataaagcg caggaagtgc tggctaccgc taatgaagtg gccgatcacg ccgatgcctt
     7981 tgccgagctg gattacaaca tattccgcgg cctggcgttt gcttccggca acccgattta
     8041 cggtctgatc cttaacggga tgaaagggct gtatacgcgc attggtcgtc actatttcgc
     8101 caatccggaa gcgcgcagtc tggcgctggg cttctaccac aaactgtcgg cgttgtgcag
     8161 tgaaggcgcg cacgatcagg tgtacgaaac agtgcgtcgc tatgggcatg agagtggcga
     8221 gatttggcac cggatgcaga aaaatctgcc gggtgattta gccattcagg ggcgataatc
     8281 ccttccgttt aaagagcaaa cccctcaaac gaggggtttt ttgttgtttt tacagatttc
     8341 ccattcttgg cgggcaacgt tccagcaact cgatgctgcc gtcttcgttt tgctgttcga
     8401 gcatcacatc aaatccccac aggcgatgca catgcttcag gacttctttg cgcccccgat
     8461 ccagcggtgc gcgattatgt ggaatataac gcagcgtcag cgaacggtcg ccgcgcaaat
     8521 ccacgttcca gatctgaata ttcggctcca gattacttaa gttatattgc gacgataacc
     8581 ggttacggat ctcccgataa ccttcttcat tatgaatagc ggaaatctcc agataattat
     8641 gccgatcatc atccagcacg gtgaagaagc ggaaatcacg catcactttc ggtgacagaa
     8701 actggctgat aaaactctca tctttgaaat cacgcatcgc gaaatgcagc gtttccagcc
     8761 agtcggaacc ggcgatatcc gggaaccagt atttgtcttc ttccgttggc gactgacaaa
     8821 tccgtttaat atcctggaac atggcgaacc cgagggcata cgggttgatg ccgctgtacc
     8881 acgggctgtt ataggggggc tggaagacca cattggtgtg gctgtgcaaa aactccagca
     8941 taaaacgttc cgttactttc ccttcatcat acagatggtt aaggatggta tagtgccaga
     9001 aggtcgcaca gccttcgttc atcacctgag tctgtttttg cggataaaaa tactggctca
     9061 ccttacgcac aatacgcagg atttcacgct gccatgattc cagcagtggc gcatttttct
     9121 ccataaaata gagcaggttt tcttgtggtt cggacggata gtggcgcgct tcagcaaccg
     9181 ttttctcttc ctcgcgcttc ggcagggtac gccagagcat attgacctga ctttgtagat
     9241 actcttcgcg acttttctgc cgggctttct cttcttgcag cgagattttt tgcgggcgtt
     9301 tgtaccggtc cacgccgtag ttcatcagcg cgtggcacga gtccagaagc cgttctactt
     9361 catcaacgcc ataacgttct tcgcactcgg taatatattt acgggcaaaa atcagataat
     9421 cgacaatcga actggcgtcg gtccaactgc ggaataagca attgttttta aagaaagagt
     9481 tatgcccata gcaggcgtgt gccatcacca gcgcctgcat agtaatggtg ttctcttcca
     9541 tcaagtaagc gatacacggg ttagagttaa tgacgatttc ataggccagc ccttgctgac
     9601 cgtgcttata cagccgttca gtctcgataa actttttacc gaatgaccag tgcggatagt
     9661 taattggcat gccgacgctg gaataggcat ccatcatctg ttctgaggtt atcacttcaa
     9721 tctggtgcgg gtaggtatcc agccggtaga gtttcgccac ccggtctatc tctgccagat
     9781 aaacatccag caggtcgaac gtccagtcgg gtccatcgct caaacgtgtg gtgtccttat
     9841 taatagaatc gatcgtcgcc atacgcgcac ctcattgttg tcggcgctct ctgtgtggag
     9901 cacctcattt caagcataga acacctgtta aaaaccgcgt cgccggagaa tttttttctt
     9961 tgcgatttct tattatcaga gtgccactaa tccgcttctg aacggaattt tatgctggat
    10021 aaaaagggcg ttcagcagga gatactaaag acgccatatt gccgcagagt cagggagatg
    10081 tgagtcagct caccataaaa aagccgtatg ttgaataata ttttcaactg agttatcaag
    10141 atgtaattag attattattc ttttactgta tctaccgtta tcggagtggc tatgcgagtt
    10201 gtcatactgg gaagtggtgt ggtaggcgtt gccagcgcct ggtacttaaa tcaggcagga
    10261 catgaggtca ccgtcattga tcgggagccg ggggccgccc tggaaaccag tgccgcgaat
    10321 gccgggcaaa tctcccccgg atatgctgca ccgtgggcag caccaggtgt gcctttaaaa
    10381 gcgattaaat ggatgttcca gcgccatgcg ccgctggcgg ttcgtctcga cggtacgcag
    10441 tttcagttga aatggatgtg gcaaatgtta cgtaactgcg acaccagcca ctacatggaa
    10501 aacaaagggc ggatggtgcg tctggcggaa tacagccgtg attgcctgaa agcattgcgc
    10561 gccgaaacca atattcagta tgaagggcgt cagggtggga cgctgcaact gttccgtacc
    10621 gaacaacagt atgaaaatgc gacccgcgat atcgccgtgc tggaagatgc cggcgtaccg
    10681 tatcagctgc tggaatccag ccgcctggcg gaagtggatc ccgcgctggc agaagtagcg
    10741 cacaaactga cgggcggcct gcagttaccc aatgatgaaa ccggagactg ccagctgttt
    10801 acccagaatc tggcgcggat ggcagagcag gcgggggtta aattccgctt taatacgccc
    10861 gttgaccaac tgctttgcga cggcgagcaa atctacggcg tgaagtttgg cgatgaagtg
    10921 attaaggccg atgcgtatgt gatggcgttt ggttcttact cgacggcgat gctcaaaggc
    10981 attgttgata ttccggtgta tccgctgaaa ggctactcgc tgactattcc aattgcgcag
    11041 gaagatggtg cgccagtatc caccattctt gatgaaacct acaaaatcgc cattacccgt
    11101 ttcgataacc gcattcgtgt tggcggaatg gcggaaattg ttggttttaa caccgagctg
    11161 ttgcaaccgc gtcgtgaaac gctggaaatg gtggttcgcg atctctatcc acgcggcggt
    11221 catgtcgagc aggcgacttt ctggacgggt ttgcgcccga tgacgccaga cggtacgccg
    11281 gttgtcgggc gtacacgctt taaaaatctg tggctgaata ccggtcacgg cacgctcggc
    11341 tggacgatgg cttgcggttc cggtcagttg ttaagcgatc tgctctctgg tcgcacgcca
    11401 gcgatcccat atgaggatct aagcgtagcg cgctacagcc gtggatttac gccatcacgt
    11461 ccgggccatt tacatggcgc acacagctaa ggaaacgaga tgacccgtcc gatacaggcc
    11521 agcctcgatc tgcaggcatt aaaacagaat ctgtccattg tccgccaggc cgcgccgcac
    11581 gcgcgcgtct ggtcggtggt aaaagcgaac gcttacgggc atggtattga gcgtatctgg
    11641 agcgcgctcg gggccaccga tggctttgca ttacttaacc tggaagaggc aataacgtta
    11701 cgtgagcgcg gctggaaggg gccgatcctg atgctggaag gatttttcca tgctcaggat
    11761 ctggagattt atgaccagca ccgcctgacc acctgcgtac acagcaactg gcagctcaaa
    11821 gcactgcaaa atgcgcggct aaaagcaccg ttggatattt atcttaaagt gaacagtggg
    11881 atgaatcggt tgggcttcca gcccgatcgc gtgcttaccg tctggcagca gttgcgggca
    11941 atggcgaatg ttggcgaaat gaccctgatg tcgcattttg ctgaagcgga acatcctgat
    12001 ggaatttccg gcgcgatggc gcgtattgag caggcggcgg aggggctgga gtgtcggcgt
    12061 tcgttgtcca attcggcggc gactctgtgg cacccggaag cgcattttga ctgggttcgg
    12121 cctggcatta ttttgtatgg cgcttcgccg tccggtcagt ggcgtgatat cgccaatacc
    12181 ggattacgtc cggtgatgac gctaagcagt gagattattg gtgtccagac gctaaaagcg
    12241 ggcgagcgtg tgggctacgg cggtcgctat actgcgcgcg atgaacagcg aatcggcatt
    12301 gtcgccgcag gatacgccga cggttatccg cgccatgcgc ctaccggtac tcctgtttta
    12361 gtggacggcg tgcgcaccat gacggtgggg accgtctcga tggatatgct ggcggttgat
    12421 ttaacgcctt gcccgcaggc ggggattggt acgccggttg agctgtgggg caaggagatc
    12481 aaaattgatg atgtcgccgc cgctgccgga acggtgggct atgagttgat gtgcgcgctg
    12541 gcgttacgcg tcccggttgt gacagtgtaa cttattgtaa gccggatcgg aggcagcgtc
    12601 ttctggttgc gaaaaaatca tccatccggc tggtcagcaa ctgtagttgt taatgtgaca
    12661 gagccattgc ccatgatagt gtccattaaa agaatggaca ctatttcccc ggaacctgaa
    12721 ctcgccgcac aggcgttcta cataaaacgc ttacgcttca ttattgactc ggagtcgact
    12781 tagataaatc acgcttcacc cttgatgcag attcgggttc atgttcagat tgttgattaa
    12841 caggctgcga gtcctgttgt ctttccagac tcaacaggcc caactgttga tccaggagct
    12901 ccaggtcctc ctgtaccttc acgcgctcca ggagttccgg gtcatccagg aactccagat
    12961 ccataaggtc ctccagctcc tcgagtcctt ccaggtcaac ctagccttcc ttgtgcaaaa
    13021 agtccaaaag gtccttcagg ccctgctgtt ttttcaggcc ctcgacggct ttgagcagcg
    13081 tcggtaaatt gtctttgtcc cacgggttac cattacagtc aaacaccttc aggctctggg
    13141 gctgcggcac ctgtaccgtt ttaaacttgt tttctccaca gtagtaaatt attagtttgg
    13201 gcggcagccg tggtgaagag atcaaatcgt tactagaaca gtcaaatact ttcaggcgag
    13261 tcaagttctc cagcatctca tcaggtattg agttaatatt gttgccggca caggatagtt
    13321 tatacaggta ctggggcatc gacggcggta aaaattgcaa gaagttttca gaacagttaa
    13381 tcactctcag gttagtcgac agctcaggca gccagataat acagttttca tagcacttca
    13441 gtaccatcag ttttgagggc tgcaccttcg gtaacaagat caagacgtta tctgtacagt
    13501 caagcaccag cagccacttt ggcatcacct tcggtaacgc ggtcaacacg tttttactgc
    13561 agtaaaattc aatcagatgt ggaggcaaat tctcagggag tgaagtcaat cccaggttgc
    13621 taagattgag catactccgt cttgtaatga gacattcctt cattctgcgc aatgcttctg
    13681 cccggttttc aatccggctt gctggcaggt tttcttccag gatattttcc ttgatttctt
    13741 ccagactttc tttcaggtct tcttggggaa tttctgactg tttgttttgc tggttttctt
    13801 ccaggatttc gtacaggttt ttttgcaggt tttcttgcaa accatcctgc tgtatagccc
    13861 attcgcacca tttggcgacc atcatgaact cgttatgttg ctgattcagg cgagtcccca
    13921 acgtgatgat ggtattgttg tccacctgta tgttcacttt gcccgcgata ctgaggtcaa
    13981 tgatctgcca gggggcatca ccccagctga atttttgcgg gctgagatac ttcatgttct
    14041 gcaaaatttg attcttgagt tccaccgaca ggaagagccg ccctggcggg cgtcgtacgt
    14101 ctgggttaac ggaagatggg ggaatggcgg gggcgaatgg gcagatgtct ttaaagctag
    14161 ccataatgct acgtttcctt gaaataaata tcctctggca gcgccggaga cgtgaatgtg
    14221 gtcaccgcaa aggagccaca ggacgtcatt tgcgctgata tcactactca tcgttgactt
    14281 tttccctggt cagaaatgcc gtgtcgggtc tggtacatgc tgtattacgg cctaggtagc
    14341 tctgacgtat taaagaaaat acgagagtga acgacccgga aataatcgtt attaagcaaa
    14401 ggaataacta taagaaatcg ctcatgcatc cggtgctaca ggcggatgaa acgcctattg
    14461 tgtgggctat gtcagtgcgg caggttgcgt ggggaatatg ttcaggcgat gtcaggcagg
    14521 ggcccctgat ggttgaccgg taatgcgggg ctggttttta ttacctgtta cagtcggaaa
    14581 aagcccttac gctgactttc ttgagccggt gttttgagtt cattagccag agcctgcaat
    14641 ggtttgtcat ccataattta accttcttct gatgttggga tgagcatatc aaaatcagga
    14701 aatgacaagt ttattacagg ctcaggctgc gttcagtaaa gcatcgcatc cggcgataga
    14761 tgctgcatta aatttaagat tcagcttcct cttcagccac ccgaacgcca atcttcaaca
    14821 cttcattatc ttctttctcg gccaccgtcc agatcatccc ggcaaactct acctggtcac
    14881 cgacaaccgg tgccgcgcct agcaactgct ggacaatttc acccagcgtt tgctgtttat
    14941 cacgatattc tcgcccgtct tccaggccat atatcagcgc aacatcagaa tatttggcgc
    15001 tggcttcgag aatgaagtca ccaaagaagc gttgatccag cgcgaccggt ggcgactggc
    15061 tgaacagttt accgagcgcc gggagatcgc gttcccgacc aattacacac aatacatcgc
    15121 cttcgcgcag tcgggtgctg ccggtaggat gaagcaactg gttatcacga aacagggctg
    15181 caatacgcgt ctcttttggc atatgcaaat cgcgcagtgc cgcacctaca caccatttat
    15241 cagcactcaa ttgataaaca aactgctccc acggattttc cggatgaata tccaggccaa
    15301 cgcgtgacac cggacgtccg acgggcggaa ccaccacttt ggcttttttc gccgcccacg
    15361 agagtgatgt tccctgcaag agcagtgaaa ccagaaccac aaagaaggcg acattaaaga
    15421 acagtcgcgc attctccagc cccgccatca tcgggaacac ggccaggatg attggcaccg
    15481 cgccgcgtaa tcccacccag ctgataaaca cgcgctcacg cagattgaaa ccgcggaaag
    15541 ggagcaatcc agcaaatacc gaaaggggac gggcgaagaa tatcatccat gccgacaaaa
    15601 tgagcgccgg aatggcaata ggcagcagat cgcttgggtt aaccaatagc cccagcacca
    15661 ggaacatggc gatttgcgcc agccaggcga ggccgtcgaa attttgcagg atgccgtagc
    15721 ggttgcgaat cggccgatta cccagcagaa aaccgcacag ataaacagcc agaataccgc
    15781 tgccttccag cgccgtggtt aaagcaaaaa ttaaaatacc gccgcttaat gccagcaatg
    15841 gatataatcc ggcaggcagg gcgatgcgat taatcatttg cagcagtaaa taaccaccgc
    15901 caagaccgat gacaatcccg aggccaaatt gctgcagaat atcgaccacg aacatccaac
    15961 tgacactgct ttcatgctgc tggatcattg caatcagggt aatcgtcaga aagaccgcca
    16021 ttggatcatt actgccggat tcaatttcca gcgtcgagcc aacacgttcg ttaagcccct
    16081 taccacccag cagagaaaag accgctgcag catcggtgga gccgacgata gcgccgatta
    16141 ataagccttc aatcaaatca agattaaaca gccacgccgc catcatgccg gttaaaccag
    16201 aggtgataag tacgcccagc gtcgccagcg acagcgccgg tcctaacgcc acacgaaagg
    16261 aactggcctg agtgcgcatc ccgccgtcga gcaaaataat cgccagcgcc aggttactta
    16321 ccatgtaggc gaaggggtaa ttatcaaacg ggataccgcc gacgccatcg actcccgcca
    16381 gcatgccgat cgccagaaaa ataaccagaa taggaatgcc aagacgggaa gaaaatgaac
    16441 taagtaatat actgctggtg acaaggatgg atcctaagat gaaaaggcta attattgttg
    16501 tggcatccaa cggtcggtta ctcctgatta cgctgtctct tatataaacc ctaccatatt
    16561 agcggcagag acagcgtttt acttagccct gaagcgtgat ttttttacat tttaagaaca
    16621 ggatgaccgc taatcgtcaa ctgagtgcct tcccgggtgt tattcagaat ggcatgtgcg
    16681 cccaacggca gcgtaaccgt gcgttgttca tgaccaaaat cgaggccggt aatgagtgga
    16741 atcgacaggc gggaacgcaa aaacgcgtac actgactcca ggttgtaacc tgcgtcataa
    16801 tcattgggcg tgctgccgct aaagctaccg agaataatcg ccttctgacg tggcaaaatt
    16861 cccgcatgat agagctgcaa cagcatacgt tcgacgcgga aagggtgctc gttaatatct
    16921 tccagcacca gaataccgtt ctcaattttt ggcatccacg gtgtaccaat cagtgaaatc
    16981 agcatcgcaa gattgcctcc ccacaacgtg ccttcggtct gacatgtcgg gccttcccct
    17041 tgccattcaa tggtgaaggt ttcattgcgt aacgccagcc agaaatggtg ctcggtaaag
    17101 gcgttcagtt catccgcacc aaagtttgct accagcatgg ggccgctaaa ggtgatgaca
    17161 ttgccttgcg ccagaagacc gcactgaatg gcggtaaaat cgctatgtcc gcaaatgagt
    17221 aacgggtcat gttgttggcg agccaccagt gcctgccagt caatatctgc cagtaaacga
    17281 ctggcaccgt aaccgccgcg tacagccagc acgatggtgt tgggggtagt cagtctggca
    17341 agggaattga gatcttccag acgctccgtt tccgtacccg caaaacgctc acaacgacgg
    17401 gcaataacct cgacgttatt gacttgatgc cccgcgtcgg taaggcgctg gataccacgc
    17461 agcgcggcgt gctgtttaat gctgtaaccc gatggggcaa ttaagtgaaa cagagacatg
    17521 gcaattcctt gctgacaaca gaaacgaaat gtatatcatg ccgcttaggt gtgccgttgt
    17581 cacctcaacg gcgattccag gctataagga tagaagaagt gaaattgaga tggtttgcct
    17641 ttttgattgt gttattagcg ggttgttcat caaagcatga ctatacgaac ccgccgtgga
    17701 acgcgaaagt tccggtgcaa cgtgcgatgc agtggatgcc aataagccag aaagccggtg
    17761 cagcctgggg cgtcgatcca caattgatca cggcgattat cgctatcgaa tcgggtggta
    17821 atccgaacgc ggtgagtaaa tcgaatgcca ttggtttgat gcagttaaaa gcttcaacct
    17881 ccggacgtga tgtttatcgc cgtatgggct ggagtggtga gccgacgacc agcgagctga
    17941 aaaatccaga gcgtaatatt tcaatggggg cggcttacct gaatattctg gaaaccggcc
    18001 cgctggcagg cattgaagat ccgaaggtac tgcgatatgc gctggtggtg tcatacgcta
    18061 acggggcagg tgcgctgcta cggactttct cgtcagatcg gaaaaaggcg atcagcaaaa
    18121 tcaacgattt ggatgctgac gagttcctcg atcacgtagc gcgaaatcat cctgcgccgc
    18181 aggctccgcg ctatatctac aaacttgagc aggcactgga cgcgatgtaa atcagtcgcg
    18241 cactttgtcc gctttttccc gggcttctcg ctcgagagag aaaataatcc gctgtaattg
    18301 ccgctccacc gtcgggctga cgttaagaaa acggaagctc agacggggag tggtgatggt
    18361 ttcattcttg ccatcaatca ctttgcgctc gctggtggag attaactggg cgtcaaagtg
    18421 aaaaacaccc cattgcccca tgttgacttc aatttgagcg aagcgcatac cttcttgtaa
    18481 ttcggcaggc tttgctgttt ccagtaatgc gcccatgccg cctaacgaca aatcatacaa
    18541 gcggaaacgt aacgtactgt tatccgccag tttggtctgg caaaaataag gcggatggag
    18601 tggggcggag atgcggaaat atcggcgtcg ttgtacaaac cataaggtgg gaggcggtac
    18661 ggtaataaat gccggaagct gcaagtattc actctgctgt agttgttcaa cagtaaactc
    18721 gactttcgca ccctgagttt cggcggtaat ggtaatgtgc tgtgccttta gcacggcgat
    18781 gttgtcttcg gcttgactgc cgaaatccag caccagttta tccggggtta ttgccagtat
    18841 tttgctgatc agctgcccgc cattccaact gagacgcaaa ggaattgcgg ctttgtgcaa
    18901 atcgcgtaac acgcccagga cggctaacgg attttgtttc aggaactgct catggtaatg
    18961 actcacgcgg aaaaactcct gactgacaaa ctgtctttgg tttatcggtc atagttaaca
    19021 aaacttaata caaatgcgtg aatatttttt acatgttgtt cttaaatcag ccgacaaacg
    19081 cccagcatcg atcccgccct tgcctatact tagagcgttg acgtaagcat ttcttgcgac
    19141 cgattcatcg aaacgagggc ctgaacatgg gaattattgc ctggattatt tttggcctga
    19201 tagccggcat tatcgccaag ctaatcatgc cggggcgtga tggtggtgga tttttcctga
    19261 cctgtattct cgggatagtc ggtgcggtgg tcggcgctat cctggtgctg ggcgtattcc
    19321 gcctcctgcg aagagaataa gattttcata aggcggatag cgatacagat gccgctatcc
    19381 gctttcacat cagaacgtat actcgacacc tgcccagaaa ttgcgacctt cttcaataaa
    19441 cccttcactg taggcatatt gggtatcaaa caggttattg accgacgcat tgacgctgaa
    19501 gccatgacct aaggtgtaat cggctcgaat gtgggtcacc gcaaaaccgg cggctttttg
    19561 tgaaccgtca ctgttgctgt agctggagga acgcgcctct tccgacagcg ttacgcttaa
    19621 cggctcccat ggtttgagtg tcatccatgc ggtcattgtc tgcgttggaa gatcggttat
    19681 cttgccgatg tctttgcgtt tggcgtcagc gtggatcaga gcgtagctca atcctacatc
    19741 cagtatattg ctgattttcc ccttaatacc ggcatccaga ccgctgtaat ccaccgtgcc
    19801 gctgttctga ttttgaatgg tatcggcatt gatattgtgc gagaggatgg catcactcac
    19861 ccggttatag taaacgctga cctcaaatcc ccagtcgcgc gtgaaggcac cattccaggt
    19921 taaatccacc ccgcgagcgc gttccggctt gagctgtggg ttaacaatcg ctatatggtt
    19981 atacgcaggt ttggatgtgg tatagcgttc tttcagcgtc ggaaagcgtg tgcggtcata
    20041 gtacgaaagc gccagagtgt cttcattggc aaagtggtat ttccccatca cctgccagtt
    20101 aaaagctgac tgattgttgt cgtcataatg ggtgatgctg ccatctttct cgtgtttttt
    20161 cgcttctacg ctatcgcgcc agtcatagct gattcccgcc acgacatcga cattatcagc
    20221 agcagcccat tgatattcac tggcgagcga ccaggtacga tcttcatagc gatcgtaagc
    20281 ggcgtgcggc gcaccttttt cccggtgtac gtcatctttc cagttaacgg caaacgacag
    20341 cagatcgttt tcacgcacat cggctgccag ttgtaatccg gcaccgtcgc tgtaatcgga
    20401 ataatggctg tagctgcctt ttttattttt caaatcagcc agcgagttgt acatcatcag
    20461 cgtattttca aaggtgtcgc gatacagccg acttttcagg gtaaaacggt cgtttagttg
    20521 ggtcgctccc tgataataaa aactttcttt gtcatactct ggccactgcc agtagcgtga
    20581 tttttgacca caatttccgc tgtatggcgg gttatctttt tcaccatcct gcttaatgta
    20641 agtcagcgtg tattcatcgt tttcgcgcgg tgtaaaaccg agcttcacaa tgccgcgttt
    20701 atcatcagcc gatgaattaa tcatcttgcc gtgtttgcct gcaatatcat tatttacacc
    20761 atgcggcagg ccgagaaaat cctgctttag ctggctaccg ctgacttgca aatacccaag
    20821 atcgctgctg gcggcaaatg aagcatgcat atcgtaggca ttgtcctgac tacggctcca
    20881 tccctggcga tatcccagac ttgcttcttc agggtggtga tattaatggc tccgcccatc
    20941 tgattaggcc catgaagcag cgacgaatac cctttggaaa cttcgactgc ccccagattg
    21001 ttggtcagaa cccgcgccag atcgaggttg ccgtcatagg gaacataaat gggcacaccg
    21061 tcgaaataga ccggcacctg ccgactatca aagccacgaa cttttacctg ttcttcgttg
    21121 cggctgcctg acttttgcag caccacgccg gggacgacac ttaatgcctg agcgacattc
    21181 tgtttatcca aggctttcat ggtgggttga tcgagaacgg tggtcgtcgt cgatgatacc
    21241 ggactggacc atacggtcag cgtgtcgctc tcctgagccg ctacggcctg ctgggtaaag
    21301 gcaagtgata atgctgtgca gagataacgt tttttgagtc tcatagaata aattccgttt
    21361 aacaaagaat taaatagagg ctgtgggttg cagactgact ttaatgtcag ctggtgccgc
    21421 gtaataaggc gcggaggtaa taaaaaggcg aatgccgcag tcgaggtaat ttttgagtgt
    21481 cgtcagatta attccgccgg tcagagccag cgtgcagtga ggagccagcg acggcgctat
    21541 ttgcgcaatt tcagttgcct gttgcggact aaatttgtcg agctgaagta cgtctggttg
    21601 tgcgcgtaac gcggcgatag cctctttcgg cgtgtcggct tcaacaacaa ttttcttctc
    21661 gggtgcgtgg cgacgtaact gagcgattgc gcctgaccag ccctgattgt catgaagaaa
    21721 atggcggtgg ttggcaaaca ataatatggt ttccgcacat ccggcacgat gaattagtcc
    21781 tccggcagcc agaattgcct gcgaagccag taagcgggtg cccggaattg cttttcgggt
    21841 gcaggcgata ttgccgtcag gataacgttc acgaagcaat gccagcattt gagcgagata
    21901 atcagaaaca ccgcaactcc actccagcac attctggact gccttccatc cctgatgaag
    21961 tgctgcggca ttaccctgcg cacggattag ccgttgaccc gcgttcgctt gtgaaccgtc
    22021 gctgaccgcg tcatcaatag ttaatcccag cgtagttaac atcttacacg cgacagaaac
    22081 gccgctgacg cagccgccct gacggtgaaa aaacgctata tagccaggct ggtgtccaat
    22141 atttaatgct cgcgtggtca ggtcacctcc ctggatatct tccagcagta acgcatcgat
    22201 ttgtgcctga gaaaggaaaa tcatcacagt gcctcctgcg gtacgctatc ccaccagatg
    22261 agcgcccagt cgcggctggc aggggcgagg gcacgagcat cttgttgttg ataccagtga
    22321 tataaccgct cacgctcatc gtcgttaagc gcgccaagac tccagctgac gttctgctca
    22381 aaacgttccc aggtgctgtt atcctgttgg caattttgtc cacggataaa atcaacgtgg
    22441 gcataaattc ccatctgata caggacgttg agcgcaaaga tatagttggg taattcgatc
    22501 acttcccgac cagccgcccg ctgaatggct ggagagacga acgaggtgct gaccagatga
    22561 gttgtataga cgcgaagccg tgcctgatta ttcagtttgc tcattgcatg gcgcatatca
    22621 gccaccagag tggagcggga agccacggca atatcgcagc gtggcaggtc actccagtcc
    22681 tcttcccagg cgcgctggat ccagtggaca ttgtcagcct tcagtgcggc ggcgcggcgg
    22741 gcggcaacgt tcagcatccc ctgactgtaa tcaacgccat aaaccgtagt gagtttgtca
    22801 gccagcgcca gcgatacggt gcctggtccg cagcccatat cgaataaggt ctgagcgcct
    22861 tgcaggtcaa ttttagcgat cagttgttgc aggtagctgt ccgttggcga ggcgcaattt
    22921 tccgccattt tctccgcccg ttgatcccag tgatcgggcg ttttttctgt gcggtgagcc
    22981 agtttgagtt gctgcagata aaggtctgca aaatcaatat catcaatgag catgattgtt
    23041 ccttagcggc taattgcaga gaggtgatga tgaatttggt cggcagaaac ccgatacagg
    23101 gccgccagtt tgttcgttgt taactgttcg gtcggtaagc cttgtgttac tcgtccatct
    23161 ggctctacct ggataatgct gtcggcaatg gcgttggcat ggagcggatg gtgagtcgac
    23221 attaacattg tcatgccgtt atttttgagc tgcgccagcg tgtccagaag ttggatctga
    23281 tgaccgaaat cgaggctgga ggcgggctca tccagtaaca acaagcgcgg ttgttggacg
    23341 agtgcacggg cgattaaagc cagctgacgt tcaccgccgc taagcgtatt ccagcgtcgt
    23401 gtcgccagat gcaaaatgcc cagcttttcc agttgctccg tggctttcag gcgttcttgc
    23461 ttaccgggaa cggaaaaagc gcctatgatg ggggccaggc ccatcaacac catatccagc
    23521 acactgaaag caaaaatacc gtcgtgcgcc tgcggcaccc aggcaatagc ctgtgcgcgc
    23581 tggcgatggc tgagtgtctg cacagggagg ctatccagta ggatctcgcc ttttaataac
    23641 ggtataacgc caagaatgga gcgcatgagt gtggttttgc cgcaaccatt cgcgccaagc
    23701 agacaacaaa ttgttccttg cgacagggtg aaggacacgt cgcgtagcac tggatggcgg
    23761 gagtaaccca gagacgcatg acgaacggca agcaaagtca ctggcgacct ccgcgtaaca
    23821 acaaaagcag gaaaaacggt gcgccaacaa aagcggtcag aatgccaagt gggatttctg
    23881 ttgttccaat gctgcgcgcc agcgtatcgg taagcaggag cagaatagcg ccagtacaca
    23941 ttgccatagg caacagctgc tgatggttat gaccggtcaa caagcgggca atgtgcggaa
    24001 cgaccaggcc gatccaacca attatgcccg ctattgctac cgtgctggcg gtgattagtg
    24061 tggcgcagac aatcaatcca aagcgcagac gcgtcacgtt cagtcccagc gaacgggctt
    24121 cgtcgtcgga aagcgtgagt aaattcatgc gccagcggag aaaaaacagg ggcaaggagc
    24181 ctatgagaat gatcggtgca gcatagcaga gatcgcgtaa ggtcacggtg gacagtcccc
    24241 ccagcaacca gaaggtaatt gacggtaact gggtgtaagg atcggccagc gttttaatta
    24301 gcgatatccc cgctccgcac aacgttccta acgcaatgcc taccagcacc agggttaaga
    24361 tcggatcgtg gcgggtcacc cgacgcgtaa tcaaccagac gcccgcgacc accatcagct
    24421 cgccgcaaaa tgcgtacagc tggatgtaaa caatgggtaa tccccacagg attgccgcgc
    24481 aggcaccaag tccggcaccg gcggagacgc ctaaaatatc cggtgaaact aagggattac
    24541 gcaacattcc ctgataggtg gttccggctc ctgccagcgc tgcgcccagt aacagcgcag
    24601 caagaatacg cggcacgcgg atttgccaga agacaatttg ttcctgcaca gacacatttt
    24661 cttgacgaag aattagcgcc agcagttgct gcatatcgag atgataagcc ccacttacgg
    24721 cggcaaaagt gatactgata gctgccagca gcaaaataca tccgtttacg attctcatta
    24781 gctcgccacc aatttttgat agtcggcatc actgagtgaa caatgccaga acagttgggc
    24841 gtaatgctgc atgtcacttt taaactggcg attgatggcg ggatccagcc aggcgtgaag
    24901 tctgcgtaat cccagaagac ggttgattcc cggcggggca tcgagccagc caaagggcaa
    24961 gccacttaaa aataagatgc gttgttccgc aacggctttc acgccctgcc agagaggatc
    25021 acgacgaata aaatctgcag taacggcctc ctgaaccaga ataatatccg gctgccagcg
    25081 caggagattt tccatggaaa cctgtgtcag gccgtggcga tcggctattt gcgcgacgtt
    25141 gtgcaaacct aataattccg ccgcctcggt atgcaacgaa ccctgtaagc ccgtttccag
    25201 ccctcgggga ccgcgcgcag cataaaagcg gaggttagcg gcgggtgagg tggcgaatgc
    25261 ctgagcttca ccaacgaagt ggctggcgag attggcttgt tctgcggcgc ggtgctcttc
    25321 gcctaacgtt ttgccaagcg ttgttaactg ttctgctgat tgcgccagtt ttccgttaag
    25381 caataaccag gggatttgtg tctgttcgct aacctgccgt gcctgggaga tcaaggtttc
    25441 atccgtattg ccgcaatcaa cgaccaaatc gggatgtaac gccataagcc cttccagcga
    25501 tagtgtgctg gcgcgcccgg cgagtcgtcc cagcctgggt aactggcgaa tgtgctctgg
    25561 caatggaatt aatgcctgac gggcaaaatc aaaagaggaa aaaccgacca ttttttctgg
    25621 cgcgacagcc agcaatagca aatcggcggg cgcgcctgcg ctgacgatgc gtgtaatgtt
    25681 ttgggcttcg ggaagagatt gcgaagcaaa acgattaact gtttgtgaaa aggacggtag
    25741 agactgcagg agcagggtag atgcaagtgc ctgcgcaaac gtccgtctgg ttatgggcat
    25801 ggtgaacatc ctgaaaagca aaatcgatat atactttcat atatatcgat aaaaatcatg
    25861 ttcaatatca aagtttagct attgaaaaca tgaagatgtg acgacttccg caagcctgtt
    25921 gaccctctct atcattaaaa atgtatttat caggtggcta atattattta aatcatttaa
    25981 ggctaaatca tttaaatatg aatgacatta ttgtttttta tcgtccacgg ttacgcgacg
    26041 ttgaagttac ttgttttaca atgagcgatt tttgatatct cgccgagaaa attatcagca
    26101 ttctctcgtt tcagtgtcgt gaatatactg ttaacggagg tgaaaagaat gctgggggtt
    26161 atacaaaacc tatgttgttg tcgtccggat tcaccgcttg ctgatagtaa gcagcgtaac
    26221 cagaagttac cgcaacaaga ctttgaggat tttaagaacg acgttattaa tgtcattaac
    26281 aacaatgaag atatttattt tcttagagag agtaatttac tgaatgcgtt tattcgaacg
    26341 tgcaaaaaaa gagatgataa cagttacgga aatatgatat ttacgcttta ttttgataac
    26401 gatatctatg ggaacagaat tatcaaagca tcgctgaacg gcggagagtt gcagttatta
    26461 agcacagggt gtagcaatga taacattctc cattttatta atgcatttct cattcgggtg
    26521 aaatttccgc tacctggacg cataaaaccg gaactctcgc caaaagccgg gcactccgaa
    26581 ggaattgacg cgttgcgctg ctctgtaacg gcatttaccg caagccattt atttcgccag
    26641 cgacaatccg cagagaacca aacgcatcag gaaacgactg aacacaataa atactgtatt
    26701 gatcaacata ttatactggc acctccagca cgcagtgagg atacactgta tcatcagcga
    26761 atcttatcac tgcggagctg gttgaacgac tttctgagcg aaataagtcg gttcccggta
    26821 acaacaacag aatccttttt taattattgt ttcgttgttt ttggtgtgat tcagaaaata
    26881 aatattttag acagtagcca gtcaggcgtt aaaaatgggg cggatagtga ggcggttttg
    26941 gctgcgctta ttgaacattt agaattgatc aatccttctg gcagattaag ccaaaatacc
    27001 cgttcggcaa tgcttcagtt acgcgaagag gaatggtctg aattcttttt ttggttgcta
    27061 aattctctgg agtgtctgga ttatgtgatt attaatttaa ctccagaaag caaaaaaacg
    27121 ttgatgagtg aacaccgtaa caatattcag gtagcaattg atgctttata tagccaacgt
    27181 cggcgtaaat cgccgggtga tgaaagtgaa acacttaccc gtagaaatga cgctatcttc
    27241 ggcaaccacg tatggcaaac atttgcgcaa tacttcccgc caggacttga aaaaccgtct
    27301 gtgtagaaaa aaccatagat atggtgttat ctgccgacaa cgccatccgg agaagccgaa
    27361 tgacgttgtc ggagtaagcg ctggttaagg tgtgggttgt gcctctttgg ttgagggttg
    27421 cgtcgttgct gacttaacgg tcggacgcgt cgccggaaca ttgtcacacg gttgctcttt
    27481 cgggcagatc aaatccagca ttttcagcgt cacgccattg gtccagccaa agccatcctg
    27541 taatggatat tcgccaccgc cgccccccgt tccggtggtg ctgacatcat atttttccac
    27601 cagctttttc tcccggtcat aggtgtgctg aacattggtc aggaagtgcc agctaatgtc
    27661 catcgccacc tctttttgcc cgtagttttg taatccttct gttgcgaccc actgtaacgg
    27721 tgcccagcca tttggcgcat cccattgttg cccacttttc accgacgtgg tgttcaggcc
    27781 gccgggttgc agcagatgtg ttttcgtcgc cgtcgccatt ttgttggcgc gatctttcgc
    27841 tgccgcattg acgtacagcg ggaacagggc ggccgcggtt aactgattgc gcactttatg
    27901 acttttcagg tcgtaatcgg cataccagcc ttgttgatcg ttccacaggt atttttcgat
    27961 ccctttttga cgggcattcg ccagcgtttc gtactggttt gccatcgcgt tatctcctgc
    28021 agctttgctg gcgcgggcga ggattttttc cattttaaac atcaggctgt tcagatcgac
    28081 cggtacgatg ctggtggtgc gtaaggtatt taactgctgc gggttgtcca tccagcgcga
    28141 gctgaaatcc cagccagacg cagcggcaga gcgcaggtcg cggtaaattt cagtggcagg
    28201 tcgattcgga ttgcttttgg cggtggcaat atcttccacc catgactctg gtcgtggcgt
    28261 atcgcgatcg tcccagtagc ggttgagaag ggtaccatcc tgaagtttta caacgcgttt
    28321 ttcctgttgt ccggcttgca ggttttcaac accgtccatc cagtaagcat attctttttg
    28381 catttgcggc aggtattgct tcaacgcggc atcgccttca tgctgcgcca gtaactctac
    28441 catcagggca aagaagggcg gttgcgagcg gcttaaatag taactgcggt tgccgttggg
    28501 aatatgaccg taagtgtcta tttcatgagc aaaattggcc accatatccg cgactttatc
    28561 ccagtgaccg ctttcggcaa gtcctaacat ggtgaagtaa ctgtcccagt aatatacctc
    28621 gcgaaaacgt ccgcccggca cgacataagg ttccggcagc ggtaacagag aatcccattt
    28681 ttcggtgttt tcggtagaac gcgttaatac cggccaaagt ccgtcaatat gttcgcgcag
    28741 tgactgcccc tctggcggaa catatttctc gccttctttc ggcagggtga aattgacgtt
    28801 aacgaaatgg cgcagatcaa atccgctctg gttttgctgc atccgataat cagcaaggat
    28861 catcagcgga tcgctgttcg gcacggcatc ggcaaaggtt ttttggtccg gaaaaagttt
    28921 ggcgttttgc acatcattaa acagcggccc taataaaata tcaggcggct gtggtgttac
    28981 cggtgtttct tctgcctgca ccgatagcgc agcgaaacac aaaaagatac aggctggaat
    29041 taacgccatt ttttgcgggc gagaaggtgc gggggatttc atcaatcatt ctcctttggc
    29101 gaaaccgaat aaaacggtat tcaagtatca gaaaacctta gttcaggatc gcactagctg
    29161 catgatcgaa atctcattct gcgaacggtc aggcatttta gaaaagggaa ttaaagggat
    29221 aaataacggc gaaatagagt gatcaaataa cgtctgttta ttagccagcc agcgacattt
    29281 ggttcgcgat tagcgaagaa aaatcatcat aaattggtaa aaaatataac aggtagttaa
    29341 aaaacatcag tgctgggtaa attgccggat gacatcagaa cgatgccatc cgaacggtgg
    29401 cttaaccctg acggttgaaa cgttgcgttt taacgtccag cgttagcgtt tcttctggtt
    29461 gtatcgcata cagtttctca ccctgctggc aaatccagcc aatccccagt tcacgggcga
    29521 ttagagcgct gtgggatacc ggacttccgg cgctaaggca gatacctttt acaaccgccg
    29581 gatccagttg cagtactgtg gaaggataaa tgttctccgc cagtagaata gttggcgagt
    29641 taaactgcgg ga
//
LOCUS       DADMEQ010000041        29418 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.041, whole genome shotgun sequence.
ACCESSION   DADMEQ010000041 DADMEQ010000000
VERSION     DADMEQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.041"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..109)
                     /locus_tag="JNZ05_001838"
     CDS             complement(<1..109)
                     /locus_tag="JNZ05_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic ABC transporter ATP-binding protein"
                     /protein_id="HAY8565094.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVH"
     gene            complement(179..1168)
                     /locus_tag="JNZ05_001839"
     CDS             complement(179..1168)
                     /locus_tag="JNZ05_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8565095.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAH
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVETPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1965..2468
                     /locus_tag="JNZ05_001840"
     CDS             1965..2468
                     /locus_tag="JNZ05_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HAY8565096.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(2548..2799)
                     /locus_tag="JNZ05_001841"
     CDS             complement(2548..2799)
                     /locus_tag="JNZ05_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HAY8565097.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(2914..3000)
                     /gene="azuC"
                     /locus_tag="JNZ05_001842"
     CDS             complement(2914..3000)
                     /gene="azuC"
                     /locus_tag="JNZ05_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HAY8565098.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            3263..3586
                     /locus_tag="JNZ05_001843"
     CDS             3263..3586
                     /locus_tag="JNZ05_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565099.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            3757..4254
                     /gene="ftnA"
                     /locus_tag="JNZ05_001844"
     CDS             3757..4254
                     /gene="ftnA"
                     /locus_tag="JNZ05_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HAY8565100.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(4291..4530)
                     /locus_tag="JNZ05_001845"
     CDS             complement(4291..4530)
                     /locus_tag="JNZ05_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HAY8565101.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            4720..5931
                     /gene="tyrP"
                     /locus_tag="JNZ05_001846"
     CDS             4720..5931
                     /gene="tyrP"
                     /locus_tag="JNZ05_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HAY8565102.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVPSPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(5993..6658)
                     /gene="yecA"
                     /locus_tag="JNZ05_001847"
     CDS             complement(5993..6658)
                     /gene="yecA"
                     /locus_tag="JNZ05_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HAY8565103.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(6854..6940)
                     /locus_tag="JNZ05_001848"
     tRNA            complement(6854..6940)
                     /locus_tag="JNZ05_001848"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6904..6906),aa:Leu,seq:taa)
     gene            complement(6953..7026)
                     /locus_tag="JNZ05_001849"
     tRNA            complement(6953..7026)
                     /locus_tag="JNZ05_001849"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6992..6994),aa:Cys,seq:gca)
     gene            complement(7308..7856)
                     /gene="pgsA"
                     /locus_tag="JNZ05_001850"
     CDS             complement(7308..7856)
                     /gene="pgsA"
                     /locus_tag="JNZ05_001850"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAY8565104.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(7913..9745)
                     /gene="uvrC"
                     /locus_tag="JNZ05_001851"
     CDS             complement(7913..9745)
                     /gene="uvrC"
                     /locus_tag="JNZ05_001851"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAY8565105.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(9742..10398)
                     /gene="uvrY"
                     /locus_tag="JNZ05_001852"
     CDS             complement(9742..10398)
                     /gene="uvrY"
                     /locus_tag="JNZ05_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HAY8565106.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            10694..10870
                     /locus_tag="JNZ05_001853"
     CDS             10694..10870
                     /locus_tag="JNZ05_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565107.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            10857..11081
                     /locus_tag="JNZ05_001854"
     CDS             10857..11081
                     /locus_tag="JNZ05_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HAY8565108.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLILQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(11114..11734)
                     /gene="sdiA"
                     /locus_tag="JNZ05_001855"
                     /pseudo
     CDS             complement(11114..11734)
                     /gene="sdiA"
                     /locus_tag="JNZ05_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(11964..12716)
                     /gene="yecC"
                     /locus_tag="JNZ05_001856"
     CDS             complement(11964..12716)
                     /gene="yecC"
                     /locus_tag="JNZ05_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HAY8565109.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(12713..13381)
                     /gene="tcyL"
                     /locus_tag="JNZ05_001857"
     CDS             complement(12713..13381)
                     /gene="tcyL"
                     /locus_tag="JNZ05_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HAY8565110.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(13396..14382)
                     /gene="dcyD"
                     /locus_tag="JNZ05_001858"
     CDS             complement(13396..14382)
                     /gene="dcyD"
                     /locus_tag="JNZ05_001858"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HAY8565111.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(14487..15287)
                     /gene="tcyJ"
                     /locus_tag="JNZ05_001859"
     CDS             complement(14487..15287)
                     /gene="tcyJ"
                     /locus_tag="JNZ05_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8565112.1"
                     /translation="MKLAHLGRQALMGVMGVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(15375..15926)
                     /gene="fliZ"
                     /locus_tag="JNZ05_001860"
     CDS             complement(15375..15926)
                     /gene="fliZ"
                     /locus_tag="JNZ05_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HAY8565113.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(15972..16691)
                     /locus_tag="JNZ05_001861"
     CDS             complement(15972..16691)
                     /locus_tag="JNZ05_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HAY8565114.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDAPQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(16856..18508)
                     /locus_tag="JNZ05_001862"
     CDS             complement(16856..18508)
                     /locus_tag="JNZ05_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HAY8565115.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIILQAGNSVLAKANQVPQQVLSLLQG"
     gene            18774..20180
                     /gene="fliD"
                     /locus_tag="JNZ05_001863"
     CDS             18774..20180
                     /gene="fliD"
                     /locus_tag="JNZ05_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAY8565116.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASRNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            20205..20615
                     /gene="fliS"
                     /locus_tag="JNZ05_001864"
     CDS             20205..20615
                     /gene="fliS"
                     /locus_tag="JNZ05_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAY8565117.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            20615..20980
                     /gene="fliT"
                     /locus_tag="JNZ05_001865"
     CDS             20615..20980
                     /gene="fliT"
                     /locus_tag="JNZ05_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HAY8565118.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            21058..22545
                     /gene="amyA"
                     /locus_tag="JNZ05_001866"
     CDS             21058..22545
                     /gene="amyA"
                     /locus_tag="JNZ05_001866"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY8565119.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(22579..22992)
                     /locus_tag="JNZ05_001867"
     CDS             complement(22579..22992)
                     /locus_tag="JNZ05_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY8565120.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            23179..24384
                     /gene="yedE"
                     /locus_tag="JNZ05_001868"
     CDS             23179..24384
                     /gene="yedE"
                     /locus_tag="JNZ05_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HAY8565121.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            24381..24614
                     /gene="yedF"
                     /locus_tag="JNZ05_001869"
     CDS             24381..24614
                     /gene="yedF"
                     /locus_tag="JNZ05_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HAY8565122.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            24721..25391
                     /locus_tag="JNZ05_001870"
                     /pseudo
     CDS             24721..25391
                     /locus_tag="JNZ05_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(25431..26639)
                     /gene="tnpB"
                     /locus_tag="JNZ05_001871"
                     /pseudo
     CDS             complement(25431..26639)
                     /gene="tnpB"
                     /locus_tag="JNZ05_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
     gene            26707..27322
                     /gene="tnpA"
                     /locus_tag="JNZ05_001872"
                     /pseudo
     CDS             26707..27322
                     /gene="tnpA"
                     /locus_tag="JNZ05_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            27650..28732
                     /locus_tag="JNZ05_001873"
     CDS             27650..28732
                     /locus_tag="JNZ05_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HAY8565123.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(29221..>29418)
                     /locus_tag="JNZ05_001874"
     CDS             complement(29221..>29418)
                     /locus_tag="JNZ05_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY8565124.1"
                     /translation="SEGISFQSILDDVRLNNALSAIQTTVKPISEIARENGYKCPSRF
                     TERFHNRFKITPRELRKASRE"
BASE COUNT         7421 a         7405 c         6885 g         7707 t
ORIGIN      
        1 catgaacctg accggcatag cagtcaaaac taatatccgt cagcgcctta acgccgggaa
       61 acgttttacc gatgccgcga aatgagagat acggggtaga ctgttgcata acgtctccgt
      121 gaatcactgg tcgttcacac cccctctgca tcatgcagag gggaattttt ccggcaaatt
      181 acttaccgcc taaacctttt ttctccagtt cttctttaaa gttgtcacgc gtgatcagta
      241 ccacgtctgt aacttcggta aattttggcg tttcaacgtc ttttgctacc cagttgtaaa
      301 gcatttcgct ggatttatag ccatgtacgt ccgggcttgg cagcagggaa ccgtagaagc
      361 cggttgcctg tgctttagac agttcgctta ctgcatccac accgttaata ccaataccga
      421 tgatatcggc tgctttaaag ccctgacctt cggttgcacg tacgccgcca agtacggtgc
      481 tgtcgttcat accgacgatc agccaatgtt taacttccgg atgttgaacc agcattgagt
      541 tggcagcgtc aaatgccccc gggatgtcgt tagatttggt aggtacctga taaatttgtt
      601 tttccgggaa tcctgcagct ttcagtgcgt ccatagaacc cgttgtacgg cggcgggcgg
      661 tatccagttc gttggcggta atcgccatca ctgcgctttc tttgacatcc cagccacgtt
      721 tctgcatctc tttatacagt tcctggccct gacgttcgcc aattttagtc gccgccatca
      781 tcaccagagg aacggtatcc attggcttac ctttggcgtt aacaaactgg tcatccactg
      841 caatgacttt catatcgtag ccatgcgctt tcgcgacgat ggcagagccg agtttcgggt
      901 ccggagtaca aataacgaaa ccttttgcgc cactggctgc caggctgtcg atcgcgttca
      961 atgttttttc gccatccggc acggcaatct taataacctc aaaccctaaa tccttcccgg
     1021 ctttatcggc aaacttccat tcggtctgga accacggctc ttccggttgc ttcaccagaa
     1081 aaccgagctt caggttctcc gccatagcgg attgtgacat aacggctgcc agaccaatgg
     1141 ctgccagggc tttagtaaat ttgtgcatgg ttctctccag ctttagtgtc gttttgtgta
     1201 gggcaaaaac gaatgacatt cgttaaatta atcggaaaac aaagcattac cttttatcta
     1261 aaagataagt gaccgtgttg acatagtttt agcgagaaat taattctcca taggagagca
     1321 atatcacatc gcagaattac agtgagaacg tgcataaatt tagcgggaaa agacataagg
     1381 aaaagccaat ttgtcagaca aattgttgaa tgcacagcag attaatccat aagattagcc
     1441 tggaaatcct tgttgtcttc ggtacccatg cgggatgtct tctttttaac cagtcaatag
     1501 gccgcattac ctggcgttga gtttttgaaa tggtgtaata accacaactc aaagatgtgg
     1561 aaaatgcgcg tcattcattt cgccattaat tatcactgtg ctcattaatt aacagaacac
     1621 gtataatgag agccatctcg caaaaatgaa aaaacgtttt ataaaatcat cacttcatca
     1681 tgaattcaaa ttcatcgatt aatatcaaca agatacaaaa agcactatca ttaaaattca
     1741 ttgcatttac attgatttca ccaatgaaat gtaaaaatat ataaacttga tgatttaagc
     1801 attttcttat aaccgttcag acgttattct tatttcagat catcgtcaga attgactcca
     1861 cgatcacatt tcggaccggc agaaaggaat tattctgcaa acagtaatta tggtgttttg
     1921 atttatcttg cacctctcca cttctggata taaggatatt aggtatggca accgctggaa
     1981 tgcttctcaa actcaactct caaatgaacc gcgagtttta cgcatccaat ctctaccttc
     2041 acctgagtaa ctggtgttct gaacagagtc tgaacggcac cgccactttc cttcgcgccc
     2101 aggcacagag taatgtgacc caaatgatgc gcatgtttaa ctttatgaag agtgtcggcg
     2161 ctacccccat cgttaaagcc attgatgttc caggtgaaaa actgaactct ctggaagaac
     2221 tgttccaaaa aacgatggaa gaatacgagc aacgttcaag cacgctggcg cagttagccg
     2281 atgaagcgaa agaactgaat gatgattcaa ccgtcaattt cctgcgcgat ctggaaaaag
     2341 aacagcagca tgatggcctg ttgctgcaaa ccattcttga tgaagtgcgc agtgcgaaac
     2401 ttgcgggtat gtgccctgtg cagaccgacc aacatgttct gaatgtcgtg tcacaccagc
     2461 tgcattgatc atacatcggc gctaatacat tgcgccgatg aagtttttga gaaaccgctg
     2521 cctcatctgt ttgaagcagc ggttttttta atgggattca ccctgtgggg taaacttgag
     2581 ttcaatcagc gcgatggctt tttggattgc ccgcatggtg accgggtctg cggcagcggg
     2641 atgatgagta aagtcgatat tcttcagctg actggacatt ttttcacgaa cttcaacggg
     2701 cgcgattaca tcgagaacat ccagaatttg tttgataacc aactggcaag ctaccacatc
     2761 agaaaccagt tcctgatcgg cattcagcgg ctgggacatc gtaaactcct gatagcattt
     2821 tgaaagccgt tatagtagcg gcttcacatc ttcagcgata gtcacatcca ccgtcatcag
     2881 gacacaaaaa aacctgccgg agcaggtttt ttgttatcgg aacatattgc ctggcggtac
     2941 gtctttgaac gtcttgcaat agttattgaa catacttttc aggattttgc gcagtttcat
     3001 cgcggcactc cgaccatttg ttatacaggt gttattgtct ttgcgcttat aatatgacaa
     3061 ccatcacaaa aatcaatctt tatgtgatac aaatcacata aatacccctt taatgttata
     3121 aaaatgataa tcaaaaaaca gcccccctat ttctgacacc tacagatggc aagaaatagc
     3181 gcctgccagg catcttttcc ggccattgtc gcagcgctgt aacgcgtaaa atagtgcttt
     3241 ctcttactct tctggctgga ccatgagact tctgattctg actctttcat taataacgct
     3301 ggcggggtgt acggttactc gccaggccca tgtgagcgaa gttgacgcgg caacaggtat
     3361 tgtacggttg gtttatgacc aggctttttt gcagcatgcc cataccgatc gttatgtcag
     3421 tcgcgggatc gccgatcgcg catgccagca ggaaggctat acccacgcga tcccctttgg
     3481 tcagccagta ggcaactgta gcctttttgc gggttcctta tgtttgaata cggaattcac
     3541 tttgtcatat cagtgccatc attccgcctt ccctgtcttt ctctaaatat tttaatcagc
     3601 gaggggatct tcgctgatta aagaaatagc ggtaatgcgt tttattccca ttcgtatttt
     3661 taataattga agtttatatt ttaccttttg caaataataa aataacaaat tatagtgacg
     3721 ccacagcaac aaatataacc tttgtggagc actatcatgc tgaaaccaga aatgattgaa
     3781 aaacttaatg agcagatgaa cctggaactg tactcttcac tgctttatca gcaaatgagc
     3841 gcctggtgca gctatcatac cttcgaaggt gctgccgcgt tcctgcgccg tcacgcccag
     3901 gaagagatga cgcatatgca gcgtctgttt gattacctga ctgataccgg caatttaccg
     3961 cgtattaata ccgttgaatc tccgtttgct gaatattcct cacttgatga attattccag
     4021 gaaacctata aacacgaaca attaatcacc cagaaaatta acgaactggc tcatgctgca
     4081 atgaccaatc aggactaccc aacatttaat ttcctgcagt ggtatgtttc tgagcagcat
     4141 gaagaagaga aactgttcaa atcgattatt gataaattaa gcctggcagg caaaagcggc
     4201 gaaggtctgt attttatcga caaagaactc tctaccctcg acacacaaaa ctaatgctct
     4261 ccggcggcag gtttctgccg ccgtttcaaa tcagtgacgg caaatcttac tctgatcggt
     4321 cgaaaaacct tcttctgaag gtataaattt gccttttgct gccagaaacg ctaccagctc
     4381 tcccgctgtc atcccttctg ccgagcacgt gtgaaaacgt gcctgttcac caaaacgcgc
     4441 tttaatcgca gcttccagac tggcatgcgt atattgctcg cctgattcaa tcatcatatt
     4501 taacacttca tgaccgtgaa tagagtccat cgttcctcct caaaaaaagc ctagcgtagc
     4561 gattaccgct tatgaagact ttgcgccagc gcaggactga atgcttttat tgtacattta
     4621 tatttacaca atatgtaacg tcggtttgac gaagcagccg ttatgcctta acctgcgccg
     4681 cagatatcac tcataaagat cgtcaggaca gaagaaagcg tgaaaaacag aaccctggga
     4741 agtgttttta tcgtggcggg aaccacaatt ggcgcaggca tgctggcaat gccgctggct
     4801 gcggccggtg ttggttttag cgttacgtta atcttgttga ttgggctttg ggcgttgatg
     4861 tgctacacgg cgctattact gctggaggtg taccagcatg ttccggcaga taccggtctg
     4921 ggcacgctgg caaaacgcta tctgggacgc tacggtcaat ggctgacggg cttcagtatg
     4981 atgttcttaa tgtatgctct gactgcagca tacatcagcg gtgccggtga attgttggcc
     5041 tccagcatca gcgactggac aggtatttca atgtcggcaa ccgctggcgt gctgttgttc
     5101 acttttgttg ccggtggcgt ggtttgtgtt ggaacatcgc tggtcgattt atttaaccgt
     5161 tttctgttca gcgccaaaat tatttttctg gtggtgatgc tggtattgct gctaccgcat
     5221 attcacaaag tgaatctttt aaccctgccg ttgcaacagg ggctggcact gtctgcaatc
     5281 ccggtgattt ttacgtcgtt tggttttcac ggtagcgtgc cgagtattgt cagctatatg
     5341 gatggcaaca ttcgtaagct acgctgggtg tttataatcg gtagtgccat ccccctggtg
     5401 gcatatattt tctggcaggt ggcgacgctt ggcagcattg attcaacaac ctttatggga
     5461 ttgctggcta atcatgctgg attaaacggg ctgttacagg cgttacgcga aatggtgccc
     5521 tctccgcatg ttgagctggc ggtgcattta tttgctgatt tagccctcgc cacgtcattt
     5581 ctcggcgttg cgttaggctt atttgattat ctggctgatt tgtttcagcg ttcaaatacc
     5641 gttggtggac gattgcaaac tggtgcaatt acctttctgc cgccgttggc gtttgcactg
     5701 ttttatccac gaggatttgt gatggcgctg ggttacgccg gtgtggcgct ggcggtactg
     5761 gcattgatta tcccttcact attgacctgg caaagcagaa agcacaatcc tcaggcgggt
     5821 taccgggtca aaggtggtcg tccggcgctg gtggtggtgt ttctctgtgg tattgctgtg
     5881 attggcgtgc aatttttgat tgcggcaggg ttgttaccag aagtggggtg atcagatagc
     5941 ctcaaattcc ttattgggtg ccagaattaa cgctgacacc caatgtgacc tcttaatgca
     6001 ggcagcactg cttaaatttc ttaccactgc cgcacggaca aggatcgtta cgccccggtt
     6061 tctcttctgc tttgatcggt tgctgaacag ctttttcctg cggatgcgcc atccagtatg
     6121 catgtaaatc aagcgccgcc agtcgaatgg catctacgct ctcttcaaac gcttctggcg
     6181 acattttctc tacccgctcg aagttttcct cagtaccgtg cagcgcaatc gcctccagcg
     6241 ctggttttaa agaatcgggc aacgttgacc agtcagaaag tgccacgccc cgcatatagc
     6301 caaagcacca ctcctcaaca atcgtcagct cgctgccatc aacttctcgc aagccgaata
     6361 acggctcaaa ctgctccggg aattcgttca gacgctctgc ggtatcggcc atatgttgaa
     6421 aagccaggtt cataaagcgc gtcatctctt tctctgacgc ccaacgcggc acatagtcag
     6481 ccccacccca cacggcaacc agccactgtt ctggttcaat ctcttgcgga gaactcaaca
     6541 ccgccgtcaa taaaccgtcc agctccgcca catcaaggat ggcgtggtca gtgttgtatt
     6601 tggtcagaat atcgtccagc cattccaact cactttcgtt taacggtccc gttttcatac
     6661 gcttttcctt gtggatctca actcgccagc acctatctta catgccggtc cgtatcagag
     6721 atactttttg agtggctttg ccggtgatta aaaattaagg agggtgtaac gacaagttgc
     6781 aggcacaaaa aaaccacccg aaggtggttt cacgacactg cttattactt tgattttatt
     6841 cttatctttc ccatggtacc cggagcggga cttgaacccg cacagcgcga acgccgaggg
     6901 attttaaatc ccttgtgtct accgattcca ccatccgggc tcgggaagaa agtggaggcg
     6961 cgttccggag tcgaaccgga ctagacggat ttgcaatccg ctacataacc gctttgttaa
     7021 cgcgccaaat tcttcaggcc tttcagccag acatccgctt gacgccgatg tcttttaaac
     7081 tggagcggga aacgagactc gaactcgaga ccccgacctt ggcaaggtca tgctctacca
     7141 actgagctat tcccgtattc atcaagcaat cagttaatca cttgatttta ttatcgtctg
     7201 gcaatcagtg ccgccgttcg atgcgttgca ttctacttac ctggcgcgat gagtcaacga
     7261 tatttttcac cacttttgat cgtttgctga aaattacgcc gaaacgatca ctgatcaagc
     7321 aaatctgcac gcgcagcgct caaatattgc aacattgacc acagagtcag taccgcagcc
     7381 acaaagaaaa gtgcaatacc ggcgtactca acccaaatgt tcggacgcca cagcagccag
     7441 gccaacgcca ccatctgagc agtggttttc actttcccaa tccaggagac ggccacgcta
     7501 ctgcgtttac ccaactccgc catccattcg cgtagcgcag aaataataat ttcacgggcg
     7561 atcatcgttg ccgccggtaa tgtcacccac cagctgtgat aatgctcggt taccagcacc
     7621 atggcgatag ccacgagaac tttatctgcc acagggtcaa ggaaagcacc aaaccgggta
     7681 ctctggttcc agcggcgtgc cagaaaacca tcgaaccagt cagtcaccgc cgcgacgcag
     7741 aaaatgagcg cggcggcaaa cggcgaccag gtgacaggca gataaaagac caatacaaag
     7801 aatgggataa ggatgacacg gaacagtgta agcaacgtag ggatattaaa ttgcataatg
     7861 acgggtaact atctgttgtc agtaagatta cccctatgtt gctacagaga catcaatgtt
     7921 tcaacgacca gaagatcttt tctgccagac cttgcgaaat acccggcact tttgcaattt
     7981 cctcgacgct ggcgttacgt aaaccttgca aaccgcccat atatttcaac aacatttgcc
     8041 gacgttttgg cccgacgcct tcaatggttt ccagggaact ggtatttttg accttcgccc
     8101 gttttttacg gtgcccgcca atcgcgtgat catgtgattc atcgcgaata tgctggataa
     8161 catgcagcgc gggtgaatct ggcggcaaac taaatccctc accttccggc tcaaagaaca
     8221 gcgtttccag tccagcctta cgatctgctc ctttggcaac gccaagtagc agcggatgat
     8281 ttttatccca tgagacatcc agttcggcga agacattttt cgcctgcgca agctggcctt
     8341 tgccgccgtc gataaggatc acatccggga tcttactgtc gtcaatggct ttaccataac
     8401 gccgacgcag cacctgattc atcgccgcat aatcatcgcc cggcgtgatg cctgtaatgt
     8461 tatagcgccg atactccgca cgcagcgggc cgttagcatc aaacaccaca caggaagcga
     8521 cggtttgttc gcccatggta tggctgatgt caaagcactc catccgcttc acttccggca
     8581 atttcaacac gctggcaagc gcggtcagtc gctggtgaac ggtagattgc tgcgaaagtt
     8641 tgctggttaa ggccgtcgcc gcattggtgc gcgcgagttt cagataacgc gccctatcgc
     8701 cgcgaggttt ggtttgaaca ttaatcttgc gtcccgccag ttctgaaagg gaatcggcga
     8761 gcagcgtttt atcgctaaga ttaaaatcga gcaggatctc acccggtaag gtgcgcatct
     8821 ggctgccttg taaatagaac tgacctacga aggtttctac cacctcgctc agttccgtac
     8881 cgccaggcac tttcgggaaa tagctgcggc tgccgagcac tttgccctga cgaatgaaca
     8941 atacgtggac acaagccatg cccgcatcga acgccacacc aataacgtca aggtcgtcgc
     9001 cggtattgga aacaaactgt ttttcggtga cgcgtcgcac cgcctgaatt tggtcgcgaa
     9061 tacatgccgc ttcttcaaac tccagattct ggctggcagt ttccatacgg ctaatgagtt
     9121 gcgtaagcac ctgatcatct ttgccagaca aaaacaggcg cacatactcg acctgctgag
     9181 cgtattcttc ttcactcacc agtccttcaa cgcacggtcc cagacagcgc cctatctggt
     9241 attgcagaca cggacgcgag cgattgcgat aaacactatt ttcgcactgg cgaatgggga
     9301 aaatcttttg cagtagcgcc agtgtttcac gtacggcata gccattcggg aacgggccga
     9361 aatattcacc tttggcatgc tttgcaccac gatgcatcgc cagacgcggg tgggtatcgc
     9421 cactcaggaa gataaaagga taagatttat catcgcgtag caaaacgttg taacgcggct
     9481 gatagagttt gatgtagttg tgttccagca acagcgcttc ggtttctgta tgcgtaaccg
     9541 ttacatcaat ttgctggatc tgggcgacca acgcttcggt tttgcgcgaa gcgaggttgc
     9601 tgcggaaata gctggaaagc cgttttttca ggtctttcgc tttgccgaca tagataaccg
     9661 taccaccagc atcgtacatg cgataaacgc ctggctggct ggttacggtt tttaaaaacg
     9721 cttttgcgtc aaactgatca ctcactgact tgataatgtc tccgcattac acagaccatg
     9781 gcgaattgcc aggtgagtca gctcaacatc gccatgaatg tttagtttac tgaacatacg
     9841 atagcggtag ctgttcaccg ttttcggact gagattgagc tgttctgaga tctcattgac
     9901 cttctggccc ttagtgatca tcagcataat ctgcaattca cgttcagaca aactggcaaa
     9961 tgggctttct gttttttctg gttcgatctg gcttaacgcc atttgttgag cgatatcaga
    10021 agcaatgtaa cgctgccctg aatagacaga acgaatcgca ctcacgactt cctgcggagc
    10081 cgcgcctttg ctgaggtagc cagcagcacc tgcctgcatg actttcgctg gtaaagggtt
    10141 ttctgtatgg accgtaagca tgatgatttt aacatcagct gtggaacgcg cgattttacg
    10201 cgtcgcctca agaccgccaa tgcccggcat actcatgtcc attagcacca cgtcaacggc
    10261 atttgtccgg caccacttaa cggcgtcttc accgcacgat gcctctccga cgacttttat
    10321 accctttata tcttccagaa tgcgtcgtat ccctgcgcgc accagttcgt ggtcatcaac
    10381 aagtagaacg ttgatcaaag gaatatctcc agaaataggg ataacgctac tgatagttag
    10441 tcattcgtat attagcggtt tttattgcaa ctttgaaacg ttaaaaatgg tgggtattcg
    10501 atttttctct cgtttttgtc aattcgattg tccacagtta tggtcacgcc cgtcaccaac
    10561 ggcttaccgg atcgtttcac agtaagccct acgatgaaat gtgacaaaaa ttgactttat
    10621 tcagcaaaaa tgaaaatcag cccgctgaat atgtaacatt aattaacccg aacaatacgg
    10681 gcaaaaaaca ttcatgataa aaatatttat tggtcattat attaacgtat tttatagcac
    10741 tgccgatatc acgctcaaaa aacaaccact gctattttta gcaaagctta tggtatactc
    10801 cgccgcctta acatttttca ccgcaaattt tcattgcaac atgacgagga aaataaatga
    10861 gtacgcctga tttttctact gccgagaata atcaagaact ggcaaatgaa gtctcctgcc
    10921 tgaaagcgat gctgacgctg atactgcagg cgatgggaca agctgacgcg ggccgcgtga
    10981 tgttaaagat ggaaaaacag cttgcgctga tcgaagacga aacccaggct gcagtatttt
    11041 ccaaaacggt taagcaaatt aaacaagcct accgtcagta atataaaacc ggctgagagc
    11101 gtgcctttca gccggttatt gacaaaatca tcgctatctc tgctgatgtc ttcccttccg
    11161 ccgtccattt cagaatttct ttttcgcgct tgctgaaatt catctctggc gtcatcacta
    11221 tttcatcatt taaacgcatc agagccatca gactttcgcg caccagtaac tgcattttta
    11281 attgcagttc atcactaaga atgggtattt cgcgcgtgct gcaacgggaa aaggacaaaa
    11341 agcccagcgc ccgatttggc agcattaaat actgagtgac accgcggcgt aaaccatgtg
    11401 cgtgcgcggc ttcccataat ggctgtgctt cgctgaataa gtcatcattc cacattaaat
    11461 ggtcctgact aaagttttca gggttcagca caggatcaat tgcgagaaaa ttttttgcct
    11521 gataataact aacccacgac tcagggtaat tggtgtagaa agccacttta ggtcgcgtga
    11581 atggtaccgg gtggcgaaca cataacgaat agtaatcgta ctccagctgc tgagcctgaa
    11641 gctctatttc atggtagacc tcttctgcgg cctccatcct ctgaaaacgc aacagcatcg
    11701 tgcgacgcca gctgaaaaaa tccgtatcct gcatagtaaa ccgcaacgcc cctgagagtg
    11761 agtatcattt ataatgataa ttcaaactat cacataaata agatttatat ataatttata
    11821 ttattcaggc aatgaattac ttttgcaagc catcgcattc tcttatgtta ttaatgagtt
    11881 atgctgattt gttaagcagt tttatcacgc ttgaaatggc gtcccgcccc gacaggtgaa
    11941 tcgtcggggc tgattttttc ttattattgc agcagaagct tctcgaggaa ctggcgggtg
    12001 cgaggctgct ggggatcggc aaataacgct tttgctgccc cctgctcgac tatccgcccc
    12061 tggtccataa agatcgcccg gtccgcaaca tcccgggcaa agctcatttc gtgtgtcaca
    12121 atcaccatcg tgcgcttttc ctgcgccagc tggcggatgg tgttcaggac ttcgcccacc
    12181 agctctggat ccagcgccga cgttggctcg tcaaacaaaa tcacctcagg acgcattgcc
    12241 agtgcacgcg caatcgcaac acgctgctgt tgacctccag acaaacgacg tggatagctg
    12301 gtttctttac cggccagccc aactttcgcc agcagctcgc gagcgcgcgc cgtggcctct
    12361 tctttcggtt cgcctttgac gatcaccggc ccttcaataa tgttttccag caccgtacga
    12421 tgcggaaaca aattaaagtt ctggaagaca aacccgacgt gctgacgcaa ctggcgaatc
    12481 agcgattttt gctgacttaa tgaacgtgca gtatcaatag tgatatcgcc aacggtgatc
    12541 gttcccgctt ccggttgttc cagcagattt atgctacgta gcaatgtggt tttgccggaa
    12601 ccactcggac cgataattgc caccacttcg ccaggcttta cctcgaggtc gataccgtgc
    12661 agcaccgtct gaccgtggaa ttttttcacc aggttcttaa cttcaatggc actcattttg
    12721 gttctctctc ctggcgatta agttgattct caaaatggtt ctgcaacgtc gataacacag
    12781 tcgccatgat ccagtagatg agcgaagccg ccaggtacat ggtgaacacc tccagcgtgc
    12841 gcgaggtaat caactgcgcc tgacggaaca gctccggcac ctggattgtc gcggccagcg
    12901 acgtatcttt taccagactg ataaagctgt tcgacagcgg cggcaacgcc acccgcgccg
    12961 cctgtggcaa aatcgcgcga cgcatggtct gccacggcgt cataccaata ctggcagcgg
    13021 cttcccactg acctttatca atagaagaaa tggcggcgcg cagcgtttcg gcggcatagg
    13081 cggcagtgtt cagcgacaga ccaatcattg ctgacggaat gggatctaat tcgataccaa
    13141 actgcggcag gccgtaatag atcataaaca gttgtgcgat gagcggcgta ccacgaaaaa
    13201 tagagatata aaaacgcgcc agccagcgca cgggccagat tggcgacagg cgcatcagcg
    13261 cgagaataaa cccgagtagt aagccaaaaa acatgccgcc gatactgagt tgcagggtat
    13321 acccagcccc tttcaacagg aacggcaagg aatcaataac cagttgtata ctttcttgca
    13381 tgagcgttcc cggagttaaa cgtggggatg ataggcgaac agcgcaggcg cgccgccggt
    13441 atgaataaac agaatcggcc cttcatcttt gaagcgtttc tgactgatac cgtcaatcag
    13501 ccccgccatc gcttttccgg tatacacagg atcaagcaga ataccttcca gacgcgccag
    13561 caatttcact gcttccatgc cttcgtcgtt cggcacgccg tagccaggtg caaaatagtc
    13621 atcccagagt aaaatttccg ctgatgcggt cagctccagt tctttcgcaa tcgcctgttg
    13681 taggttaacc actttcggca attgatcggc aacggaacgc gacacggtca cgccaatcag
    13741 ttcgctttca ggcatcaggt gttccagccc aacagccagt ccggcgtgag ttccggcact
    13801 gcccgatgcg actaccaccg acgaaatatt aaccgcccct tcacactgtt gcgcgatttc
    13861 cagcgcactc tccacataac ctagcgcgcc aagagcatta gaaccgccaa ccggaatgac
    13921 atacgggcga aagccttgtg cttcgactcg cgtcgccagc tcttccagtt gggcattggg
    13981 atcggtcagt gcgtcgcaca tttcaatctg ggtattgaac agatccagca acaaacgatt
    14041 gccgttggtt aaatagtttt ctgcggttgt gccaatagga ttttccagca gcgccacgca
    14101 gtgcagaccg agtttcgccg cgactgcggc agtctggcgc acatggttag actggatcgc
    14161 gccggcagta atcagcgtat cggcaccttc gcgcagggca tctgccgcga gaaattccag
    14221 cttacgtaat ttattgccgc ccattgccat gggggtgacc tcatcccgtt tgatgaaaat
    14281 ttcccgtcca agataatcag aaaagcgcgg cagatattcg agcggcgttg gcgcgccgat
    14341 aaactccaac cgtggaaaac gggttaaatt atgcagtggc ataacagcct ccgatgtgtg
    14401 ttgttgtgat tttcttatta tgcacgctga aaacgcgtaa ataaaaaagg cgctagtgaa
    14461 agcgcccttt tttgtcatta tgctgattat ttggtcacat cagcaccaaa ccatttttcg
    14521 gaaagagctt gcagagtgcc gtctttttgc atttccgcaa ttgcatcatt caccgctttc
    14581 agcaggtctt catttccttt acgcagcgcc acgccagact cctgacggga gaatgcttca
    14641 ccggttactg ccagcgtatc gttggttttc ttcaccagat ccagcgccgc cagacgatca
    14701 acgaggatcg catcgatacg ccctacgcgc agatcctgat atttggtcgg gtcatcatca
    14761 taggtacgca catcgacacc ctgaacattc tgccgcagcc actcttcata gttggtgccc
    14821 agaccgacac ccactttttt gcctttcaga tcagcagccg ttttaatggt gccttcgtta
    14881 ccttttttca ccagcgcctg aataccggaa atggtgtaag gggtagagaa atcgtatttt
    14941 ttcttgcgct cgtcagaaat ggtgacctga ttaatcacca catcaatgcg tttagagtcc
    15001 agcgacgcca gcataccgtc ccatttggtc ggttttagtg acgcctcaac gccaagatgt
    15061 tttgccagct gttgggcaaa ttccacttca aaaccggtta atttgccgtc atccccctga
    15121 aaactgaacg gcggataagt gccttccagc cctaccagca gcgtgccgcg ctctttgact
    15181 ttattaagca gaccttcatc tgcaaaactt ttaacgctca tgcccgcaac cagcgccacg
    15241 cccatcacac ccatcaatgc ctgacgtccc agatgtgcta atttcatatt caccccgaat
    15301 gttgttatgt ctgtttgcag tgtagagcca ttgtttgtaa acacaaaaac aactccgcta
    15361 catcttattc ttatttaata tatatcagaa gcaggcaggc tggaggattt ctgcacaagt
    15421 ccggtacagg tttggcgctg atagtgttga tacttccgta atgcgatgcg gtaattgttg
    15481 gtgctggtgg tgggcagcca cggggcgagg atttcatcaa ggaaaccgtc ctgcagctga
    15541 tcgagggaaa tattttgctc atgtagatga ttacccaaac ggcgcagacg aacgacatat
    15601 tcacgcaccg tacctgggct catttcagtt tgctcaaaaa gaaatttctt aaagccgata
    15661 atatcgaaaa aatcactctg cgttttgcaa tgtaaatcac cgcaaaatcg gcacaatgcc
    15721 gcccatgatt gttgttccgt ttgccagcct ttttcatcaa gcagcgtgtc caggcgggaa
    15781 atctcgattt tattcactat tttgccgtcg cgaactaagg taatgcgatc gagtaattta
    15841 cggcaatgcg cgcaatgggt ctggctgtgt ttaaagtctt taagatagcg gcttaatggc
    15901 cgtcttttca ggtgctgcac catcattaag aactcctggt agtcaaagtt aaagtgcggc
    15961 atttactgac gttataactt acccagttta gtgcgtaacc gtttaatggc ctggctgtgt
    16021 aactgactga cccgcgattc cccgacctcc agcaccgcgc caatctcttt gagattcagc
    16081 tcttcctggt aatagagggt taataccagt ttttcgcgct ccggcaacgt ttcgatggct
    16141 tccatcaccc gctggcgcag attactgtcc agcagttgtt gtagcgggtt ttctcgctga
    16201 tgatcatcag taaccagttc gatgctatcg ccgtgctctt cacgccactc atcgtaggag
    16261 aagagctggc tgttattggt gtcgagcagc atttgacgat aatcggcaat atcgatccct
    16321 aaacgttccg ctacctcagt ttccgtggcg ttgcggccaa gttcctgctc cagttgccct
    16381 attgcctgtg ccacttcacg cgcgttgcgt cgcacgctgc gcggcaccca gtcacggctg
    16441 cgaagttcat ccagcatagc gccacggata cgctgcactg cgtaagttgt aaatgccgtt
    16501 ccttgtgggg cgtcatagcg ttcgacagca ttaagtaacc caatgccgcc cgcctgtagc
    16561 agatcgtcaa gttccacgct cgcgggcagt cgaacctgca ggcgcaatgc ttcgtgacgc
    16621 accagcggga cataacgctg ccacagcgag tgtttatcca ttacaccttc agcggtatag
    16681 agtgaattca cgataaacag ccctgcgtta tatgagttat cggcatgatt atccgtttct
    16741 gcagggtttt taatcggacg attagtgggt gaaatgaggg gttatttggg ggttacgcaa
    16801 aaagccgaat gtaaaaaacc ccgtcggtgg cggggtttga gaggcattca gacgattaac
    16861 cctgcagcag agacagaacc tgctgcggta cctggttggc ttttgccagc acggagttac
    16921 cggcctgcag gataatctgc gctttcgaca tgttggacac ttcggtcgca tagtcggcgt
    16981 cctgaatacg ggactgcgct tcagacaggt tggtagtggt gttgttcagg ttggtgactg
    17041 cggaatccaa acggttttgc accgcaccca gggaggagcg gaatttgtcg atggagctga
    17101 tggcttcatc cagagctttc aggggatcgg ctgtcgtagc tgcttttgtt tcagcatctg
    17161 tcgtcaattt accatcagca tctttataaa ctgccttacc gctgccgtta gtcactgaac
    17221 catcatcctg taggtaaaaa tctacagtat cagatgtggt caacgcctcg ttgttatcaa
    17281 cataaaccga atcaacacct tttgttacag cgccagttgt gccgttgacg ctataacctg
    17341 cagtagtgaa atcaacattg tttcccgagg ttgatacagc tttatcgaaa gctgcctgat
    17401 caacttttgc acctgttact gaataagtaa tagtgtccgg cgtagtacta ttaccggtaa
    17461 aggagataga ggtaccgcca atgtcaatgg ttgtcgcttt ggtattattg gctgcaagtg
    17521 tggacagact agcctcagtc aaagaagcac cagagccgtt tttcgttaag cgcccagttg
    17581 tatcaatgta gagtttatct ccattgcttg ccgtaatttt accatcactg ctgatctgaa
    17641 catcctgtgc agaaccatcg atagtaatag tgcccttagc ggtatcacca actcccgggg
    17701 tcaaatattt ctgaacatcg gcagctgaag ccgttgtggt atcaaaagag tatgacttac
    17761 ttgcactgtc atacttgtaa ttcgttgcac tagccgccgt tccgaagccg ttattaatgg
    17821 ttgcctgaac agtatcacca tcactaactc cagccagcaa atcagacgct ttagcagcat
    17881 cgtaacccgc actcacagta tatttgttgc cgaccactgt tgcattagca gctaccaagt
    17941 cagctttaga tgcagcagta ttagccacag ccccgccacc attcacatta aacccattca
    18001 gccccagcgt atcagagtca attttcttca gatcaatagt gatagtctgg ccgtcattcg
    18061 caccaacctg aattttcatc gaaccgtctt ttgccagtac gttcacgccg ttgaactggg
    18121 tctgaccgga tacgcggtca atttcgtcga gacgggattt gatttcgtcc tgaatggagt
    18181 ccagatccga atcagagtta gtcccggtag aagcctgaac cgtcagctca cggatacgct
    18241 gtaagttgtt gttgatttcg gacagcgcgc cttcagtggt ctgtgcaaca gaaataccgt
    18301 cgttggcgtt acgtgcagcc tgagtcaggc ctttaatgtt agaagtaaaa cggttagcaa
    18361 tcgcctgacc cgcggcgtca tccttcgcgc tgttaatacg caagccagaa gacagacgct
    18421 cgatagaact cgacagcgca gactggttct tgttgatatt attttgagtg atcagcgaga
    18481 ggctgttggt attaatgact tgtgccatga ttcgttatcc tatattgcaa gtcgttgatt
    18541 acgttttggg tttccacccg tcggctcaat cgccgtcaac cctgttatcg tctgtcgtaa
    18601 aacaaccttt agaatttttt tcacaaacag ccattttttg ttagtcgacg aaatactctt
    18661 ttctctgccc cttattcccg ctattaaaaa aaacaattaa acgtaaactt tgcgcaattc
    18721 aggccgataa ccccggtatt cgttttacgt gtcgaaagat aaaaggaaat cgcatggcaa
    18781 gtatttcatc gctgggagtc gggtcaggtc tggatttaag ttcaatcctt gatagcctca
    18841 ccgccgcgca aaaagcgacg ctaaccccta tttcaaatca gcaatcgtcg tttaccgcta
    18901 aacttagcgc ctacggtacg ctgaaaagcg cgctgacgac tttccagacc gccaatactg
    18961 cattgtctaa agccgatctt ttttccgcca ccagcaccac cagcagcacc accgcgttca
    19021 gtgccaccac cgcgggtaac gccatcgccg ggaaatacac catcagcgtc acccatctgg
    19081 cgcaggcgca aaccctgacc acgcgcacca ccagagatga tacgaaaacg gcgatcgcca
    19141 ccagcgacag caaactcacc attcaacaag gcggcgacaa agatccgatt accattgata
    19201 tcagcgcggc taactcatcg ttaagcggga tccgtgatgc catcaacaac gcaaaagcgg
    19261 gcgtaagtgc gagcatcatt aacgtgggta acggtgaata tcgtctgtca gtcacatcaa
    19321 atgacaccgg ccttgataat gcgatgacac tctcggtcag cggtgatgat gcgctacaaa
    19381 gttttatggg ctatgacgcc agtgccagca gaaacggtat ggaggtctcg gtcgccgccc
    19441 agaatgcgca gctgacggtc aacaacgtcg ccatcgagaa cagcagcaac actatcagcg
    19501 acgcgctgga aaacatcacc ctgaacctga acgatgtcac cacgggcaac cagacgctaa
    19561 ccatcactca ggacacctcc aaagcgcaaa cggcgattaa agactgggtg aatgcctata
    19621 actcgctaat agataccttc agcagcctga ccaaatacac cgccgtagat gcgggagctg
    19681 atagccagag ttctagcaat ggcgcactgc tcggcgactc cacgctgcgg acgattcaga
    19741 cgcagttgaa gtcgatgctg agtaataccg tcagttcttc cagctataaa acgctggcgc
    19801 agattggtat cacgaccgat cccagcgatg gcaaactgga actggatgcc gacaaactca
    19861 ccgctgcact gaaaaaagat gccagcggcg taggtgcatt gattgttggc gatggtaaaa
    19921 aaaccggcat cacaaccacc atcggcagca acctgaccag ttggctttcg acaacgggca
    19981 ttattaaagc cgctaccgat ggcgttagta aaaccctgaa taagttaact aaagactaca
    20041 acgccgccag cgatcgcatt gatgcgcagg tcgcgcgcta caaagaacaa tttacccaac
    20101 tggacgtttt aatgacctcg ttaaacagca ccagcagcta cttaacgcag cagttcgaaa
    20161 acaacagtaa ttccaagtaa tcattattca tcgggagaca ggtcatgtac gcggcaaaag
    20221 gcacccaggc ctatgcgcaa attggcgtcg aaagcaccgt aatgagcgcc agccaacagc
    20281 agttggtcac catgctattt gatggagtgc tgagcgcact ggttagagcg cgactgttta
    20341 tgcaggacaa caatcagcaa ggcaaaggcg tctctttgtc aaaagcgatc aacatcattg
    20401 agaacggact gcgggtgagt cttgatgaag agagcaaaga cgaattaacc caaaacttaa
    20461 ttgctcttta tagctatatg gtcaggcgtt tgctgcaagc caatttacgc aacgatgtct
    20521 ccgcagtcga agaggtggaa gcattaatgc gtaatattgc cgatgcctgg aaagagtctt
    20581 tactctcccc ttcttcgatt caggacccag tctgatgaac aatgcaccgc atttgtattt
    20641 cgcctggcaa caactcgtcg aaaaaagcca gctcatgtta cgcctggcaa cggaagaaca
    20701 atgggacgaa ctcatcgcta gcgaaatggc gtatgtgaat gcggtgcagg agatcgcaca
    20761 cctgactgaa gaggttgccc cgtccactac gatgcaggag cagctccgcc cgatgctgca
    20821 cctgattctc gacaacgaaa gcaaggtaaa gcagttatta cagattcgga tggatgaact
    20881 ggcgaaactg gtcggtcagt catcggtgca aaaatcggtg ttaagtgcct atggcgatca
    20941 gggcggcttt gtgctggctc cgcaggataa cctcttttga atcttaatga gccgatggct
    21001 cgcgaataat ccgattacgg ctacgcttct aatattcccc ttgaatggag tcgaagaatg
    21061 cgtaatccca cgctgttaca atgttttcac tggtattacc cggaaggcgg taagctctgg
    21121 cctgaactgg ccgagcgcgc agacggtttt aatgatattg gtatcaatat ggtctggttg
    21181 ccgcccgcct ataaaggcgc atcgggcggc tattcggtcg gctacgactc ctatgattta
    21241 tttgatctag gtgagtttga tcagaaaggc agcatcccta ctaaatatgg tgataaagca
    21301 caactgctgg ccgccattga cgccctgaaa cgtaatgaca ttgcggtgct gctggacgtg
    21361 gtagtcaacc acaaaatggg cgcggatgaa aaagaagcta ttcgcgtgca gcgtgtaaat
    21421 gctgatgacc gtacgcaaat tgacgaagaa atcattgagt gtgaaggctg gacgcgttac
    21481 accttccccg ctcgtgccgg gcaatactcg cagtttatct gggatttcaa atgttttagc
    21541 ggtatcgacc atatcgaaca ccctgatgaa gatggcattt ttaaaattgt taacgactat
    21601 accggagaag gctggaacga tcaggttgat gatgaactcg gtaatttcga ttatctgatg
    21661 ggtgagaata tcgattttcg caatcatgcc gtgacggaag agattaaata ctgggcgcgc
    21721 tgggtgatgg aacaaacgca atgcgacggc tttcgtcttg atgcggtcaa acatattccg
    21781 gcatggtttt ataaagagtg gatcgaacac gtacaggaag ttgcgccaaa gccgctgttt
    21841 attgtggctg aatactggtc gcatgaagtc gataaactgc aaacgtatat tgatcaggtg
    21901 gaaggcaaaa ccatgctgtt tgatgcgccg ctgcagatga aattccatga agcatcgcgc
    21961 atgggtcgcg actacgacat gacgcagatc ttcactggga cgctggtgga agccgatcct
    22021 tttcacgccg taaccctcgt tgccaaccac gacacccaac cgttgcaagc cctcgaagca
    22081 ccggtcgaac cgtggtttaa accgctggcg tatgccttaa ttttgttgcg ggaaaatggc
    22141 gttccttcgg tgttctatcc ggacctctac ggtgcacatt acgaagatgt cggtggtgac
    22201 gggcaaacct atccgataga tatgccaata atcgaacagc ttgatgagtt aattctcgcc
    22261 cgtcagcgtt tcgcccacgg tgtacagaca ttatttttcg accatccgaa ctgcattgcc
    22321 tttagccgca gtggcaccga cgaatatccc ggctgcgtgg tggtcatgtc gaacggagat
    22381 gatggcgaaa aaaccattca tctgggagag aattacggca ataaaacctg gcgtgatttt
    22441 ctggggaacc ggcaagagag cgtagtgaca gacgaaaacg gcgaagcgac cttcttttgc
    22501 aacggcggca gcgtcagcgt gtgggttatc gaagaggtga tttaaatcca tccccggcgg
    22561 caagccgggg agatttcatt acggcagtgg cgtcggtaac ggcgctttat ccagcgcggc
    22621 ggcacactct gccgtcgggc gatccacacg ctccatcgtc ataccatcgt actcaatggt
    22681 gttaccgtca cgctcaacct cgtacagctc acgttttacg gtaacgttag tgagatcatc
    22741 agacatcaat gtcaacttac ccggcacggc aatcccccgc tgccactgac ggcaatccaa
    22801 ggtatcgccc tctttagtga caatcaggct gcctatcgct tccggactga ccagcgcacg
    22861 ttgcggccct ttggtctgcc agtagcccgc cagccagtcc ggcgcggggg ttttcacgac
    22921 attgttatag ttttcgactt cggcacagcc cgccagcaac atgagcgcgc ctgcaattgc
    22981 gagttttttc atcattcgtc ctgcatgaga agaaaagagg attgtggcat taaagctctg
    23041 atgtcgccag cctttccagg ttcagagaga aatttgatct aactattatt tttgtgaatt
    23101 attaccccga aaataaattt gcatggtgta tgattcgcgg ctcttgtttc aaaccttctg
    23161 agttcagagg ctacattcat gtcatggcag caattcaaac acgcctggtt gattaaattc
    23221 tgggcaccca tccctgcggt catcgcggcg ggtattctct ccacttacta ttttggcatt
    23281 actggcacct tttgggctgt cacgggtgaa tttacccgtt ggggcggtca gctcctgcag
    23341 ctgtttggcg tccatgctga agagtggggt tattttaaaa ttatccatct ggaaggatcg
    23401 ccattaaccc gcatcgacgg aatgatgatc ctcggtatgt ttggcggctg ctttgctgct
    23461 gcgctgtggg ccaacaatgt caaactacgc atgccacgca gccgtatccg cattatgcag
    23521 gccatcattg gcggcattat cgccggtttt ggcgcgcgtc tggcaatggg ctgtaacctg
    23581 gcggcgttct ttaccggcat tcctcagttc tcgctgcatg cctggttctt tgccatcgcc
    23641 actgccattg gttcatggtt tggcgcgcgc tttaccctgc tgcccatctt ccgtattccc
    23701 gtgaaaatgc agaaagtttc tgccgcctca ccgctgacgc aaaaaccgga tcaggcgcgg
    23761 cgtcgttttc gtctcgggat gctggtcttt ttcggcatgc tgggctgggc gctgctcacg
    23821 gcgatgaacc aaccaaaact ggggctggca atgctgtttg gcgtcggctt tggtttactg
    23881 attgaacgtg cgcaaatctg ctttacttcg gcgttccgcg atatgtggat caccggacgt
    23941 acccatatgg cgaaagcaat cattatcggt atggcggtga gtgccatcgg gatcttcagt
    24001 tacgtacagt taggcgttga acctaaaatc atgtgggcgg gaccaaatgc ggtaattggt
    24061 ggtttacttt ttggttttgg catcgtgctg gcaggcggct gcgaaaccgg ctggatgtac
    24121 cgcgcggtag aaggccaggt gcactactgg tgggtcggtc tgggcaacgt gatcggctca
    24181 acgattctgg cgtattactg ggatgatttc gctccggcgc tggccaccga ctgggacaaa
    24241 atcaacctgc tgaaaacttt tggtccgatg ggaggcctgc tggtgacata tttgctgctg
    24301 tttgctgcgc tgatgttgat tatcggctgg gaaaaacgct tcttccgccg tgcggcaccg
    24361 cagactgcta aggagatcgc atgaaaaata tcgttcctga ttaccgcctt gatatggtgg
    24421 gtgaaccctg cccttatccg gcagtcgcaa cccttgaggc gatgccgcaa ttaaaaaaag
    24481 gtgagatcct ggaagtggtg agcgactgtc cgcagtcgat caataatatt ccactggatg
    24541 cgcgtaatca cggctacact gtgctggata ttcagcaaga cgggccgacc attcgttatt
    24601 taattcagaa gtaatccctc attcccgctg tggcccacag cgggaacatc gttacctctg
    24661 acacatagat tttacccgcc ccctctccta ttcttttcag tatgatctca ggaggttgct
    24721 atgtgtggac gctgtgccca atcccaaacg cgtgaggatt accttgcgct tctcgcggaa
    24781 gatattgaac gcgatattcc ctacgatccc gaacccattg gcagatacaa tgtcgcgccg
    24841 ggaaccaaag ttcttctgct cagtgaacgt gatgaacacc ttcatctgga tccggttttc
    24901 tggggatatg ctcccggatg gtgggataaa ccgccgctga ttaacgcccg cgtagaaact
    24961 gcctcaacca gtcgcatgtt taaaccgctc tggcaacatg gtcgggcaat ctgttttgcc
    25021 gatggctggt ttgagtggaa aaaagaaggc gacaaaaaaa gccttatttt atctatcgcg
    25081 ctgacggaca acctgttttt atggccgcga taggtagcat accgtttgag cgtggtgacg
    25141 aagccgaagg atttttgatc gtcactgcgg cggcagatca aggtctggta gatattcatg
    25201 accgccgccc gctggttctg tcgccagaag ctgcacggga atggatgcgg caagacattg
    25261 gtggtaaaga agcctcagaa atcgctacca gaagctgtgt gccagcaaac cagttcacct
    25321 ggcaccctgt gtcgcgcacg gtgggtaatg tcaaaaacca gggcgcggag ttaattcaac
    25381 ctgtttgttg acatcctcca cgccctgaat gacgaggccc cctgctacgt tcaggctgtc
    25441 gcctgaatca tttcggtggg ttcctgcttc aacgagcggc ctgactgcac catctctcca
    25501 caggcaagaa cggcgtgccc cgccgctaaa atgttacgag cgccatttac atcggcgttc
    25561 gctgtatatc cacatacctg gcatctgaat ttactttgtg acaggcgatt ttctttcgct
    25621 gtatgaccac agcacgcgca acgctggctt gtgtacgctg gcggaacagc aagcacctga
    25681 ccgccacgcc agagctgctt gtactcaagc tggcggcgca tttcatacca gccctgatcc
    25741 agtatcgaac ggtttaaacc tgattttgcc cggacattgc gccccggctg gctgaccgta
    25801 cccgctgctg actttgacat gttgctgacc ttcaaatcct caatgactat cattacgtgg
    25861 tttttgctga cggtcgttgt gactttgtga aggtagtccc tgcggatatt tgcgatacag
    25921 gaatgcagtc gctgtatttt gcgtttctgc ttctgccagt tgttgctgaa tttgaccttg
    25981 cggcttaact gtcgctgaag tctcgccagc ttcttctggt ttttctgaaa actgtttaca
    26041 ggctcaaaga ctgtgccatc tgacagcgag gcaagtttag ccacgccagc atccagcccg
    26101 accattgatg ctgaagggtg aaccggagtt gatacttcac tttctgcctg aatactgatg
    26161 taccacttac cgcaggactg gctgacagtg acatttttca caacacccgt gacctgacgg
    26221 ctgttccggt agcgcatcca gccaagtttt ggcagaaaaa tacggctgtt ttcctgatcg
    26281 agcttaacgc cctgcggata gcggaatgca tcattctgtc accgcttttt gaatcagggg
    26341 aacgccgacc gcttctggaa aaagtttttg caggcctgct cagggtcttt cagtgactgt
    26401 tgcaatggct gtgacggagc atctttaatc cattgcgttt cagtggcatt tttccactca
    26461 accaatcagg aagccatttt cgcgtaaggg atgtctttgt taccggcctt atgattctca
    26521 ttctgacgtg ccagagtacg actgaaaacg aaacgacatg cgcctgcgaa gcgcctcatt
    26581 tcacgcgcct gttgaccacc gggtcttaac cggaatttga atacctgtag tcgtttcaca
    26641 gggcaaatta tactttggtc tgtgaaaaaa actgatatcc gacgcggaag gcactgtgtt
    26701 ttcctgatgc atgctcaccg ggtctttgtt accaagtacc gacgccagat ttttgaccat
    26761 gacgcgacag aaaaactacg tacttacttt tcaaatgtat gtgctgattt tgaagctgaa
    26821 ctggttgaaa tggatggcga accagagcac gtccatctgt taattaacta tccccccaaa
    26881 ctggcgatat ccagtctggt aaacaaacag cctcaaaggc gtatcgggta ggttactgcg
    26941 acgggatcgg ccagatattg ctgccaggta ttaccgcaaa gatgttttgt ggcgtcccgg
    27001 ctattttgcc agtagctgcg gaggtgcgcc aatatccgtc atccgccaat acattgaaca
    27061 acagcaaaca cctggtcagg tgaaaaaacg agccttatat ccccgccctg aaggacgggc
    27121 ttttacggtg cactggataa gcctggagat tatctacacc tgcatggaca tcattgtact
    27181 atgccccgta catgccccat cacatcctca acatcaaagt attagagcct tgcaaaccat
    27241 tatgcgtctc agtttgtctc atctatctta cgcaaaaaaa atagaactaa tatcaagcgg
    27301 cgctatattg cgtattaaat aaaaccacct caattacatt aataaatcta aataaatatc
    27361 atgcaaataa tttccatggt tatttttagg ctatttaaat taaaaaaata aattatgagt
    27421 caatcataaa taacaacctt taatataatt tgactaccaa atttatattc gttcacaaaa
    27481 tagtcatgca ttgtgtgcaa tagaaacagt tcagataaaa atagggatct acttcacaat
    27541 ttaaatgaga aactaaaact tacatcttga aataatctca ttgattggat gaatatttgt
    27601 agtgcagtga catcattttt aaataatagt tcaaataaaa gggctcacga tgaaaaaatt
    27661 aacagtggca atttctgctg tagctgcatc agtactgatg gcgatgtctg ctcaggcagc
    27721 agagatttac aataaagaca gtaacaagct ggatctgtac ggaaaagtta atgctaagca
    27781 ctacttctct tctaacgatg cagatgatgg cgacacaact tatgttcgtc tgggcttcaa
    27841 aggcgaaact caaatcaacg atcaactgac tggtttcggt cagtgggaat atgaattcaa
    27901 aggcaaccgt gctgaatctc aaggttcttc caaagataaa acccgtcttg ccttcgctgg
    27961 cctgaaattc ggtgactacg gctccatcga ttatggccgt aactacggtg tagcatacga
    28021 tatcggtgca tggaccgacg ttctgccaga attcggtggc gatacctgga cccaaacaga
    28081 tgtgttcatg actggtcgca ccacaggtgt tgcaacctat cgtaacaacg acttctttgg
    28141 tctggttgat ggtctgaact ttgctgctca gtatcagggt aaaaatgacc gcactgacgt
    28201 aactgaagct aacggtgatg gtttcggttt ctccactact tatgagtatg aaggattcgg
    28261 tgtaggtgca acctatgcta aatctgaccg cactaatgat caggttatct acggtaacaa
    28321 cagcctgaat gcatctggtc aaaatgctga agtatgggca gctggtctga aatatgatgc
    28381 gaacaacatc tatctggcta ccacctattc tgaaacccag aacatgactg tttttggtaa
    28441 taaccatatt gccaacaaag cacaaaactt cgaagtagtt gcacaatatc agttcgactt
    28501 cggtctgcgt ccgtccgttg cttacctgca atctaaagga aaagacttgg gtgcgtgggg
    28561 tgatcaggac ctgattgaat atattgatgt aggtgcaacc tattacttca acaaaaatat
    28621 gtccactttt gttgattaca aaatcaacct gattgataag agcgatttca cgaaagcatc
    28681 tggcgttgct accgatgata tcgttgctgt aggattggtt taccagttct aatctgatta
    28741 ctcaaagata tgttgtggga ggctttgcct ccccaacata taagtggcgc cctcaagcca
    28801 ctttccttag aagcactatc ttgcttctga ctatataaat taccctttat tttctgggac
    28861 gtttcaacgc cccattttta ataatactca gtgaacaatt aacgtatcaa ttagatttaa
    28921 taacaacgat atccgtacct aacctgatat ctaatgtcat aagcatccct ttaattatcc
    28981 cctcaacctc ttgtaaattt cccactcttg agctccacat agtccaaacg ccggatgcaa
    29041 aaaaaatcac acgtgcatct ctgaaattgt gatggaggaa gtgagtatca attatatgtg
    29101 cttataaacg ttcccgattg gcacagaagt caccggattg ttcaggctcc aataggttga
    29161 gtatgtcgta tttattttcc aaaataaaaa tcgatgctgt tgctccttaa taaaatactt
    29221 ttactctctg gacgcttttc ttagctctct tggtgttatc ttaaaacgat tatggaacct
    29281 ttcagtaaaa cgagaaggac acttgtaacc attttccctg gcaatctcgc tgatgggttt
    29341 tactgtcgtt tgtatagcag acaacgcatt atttaacctc acgtcgtcca atatactttg
    29401 aaaacttatt ccctcgct
//
LOCUS       DADMEQ010000042        29323 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.042, whole genome shotgun sequence.
ACCESSION   DADMEQ010000042 DADMEQ010000000
VERSION     DADMEQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.042"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            26..2293
                     /gene="adiA"
                     /locus_tag="JNZ05_001875"
     CDS             26..2293
                     /gene="adiA"
                     /locus_tag="JNZ05_001875"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HAY8565125.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGKSEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     INEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVIHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            2618..3379
                     /locus_tag="JNZ05_001876"
     CDS             2618..3379
                     /locus_tag="JNZ05_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8565126.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            3516..4853
                     /gene="adiC"
                     /locus_tag="JNZ05_001877"
     CDS             3516..4853
                     /gene="adiC"
                     /locus_tag="JNZ05_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HAY8565127.1"
                     /translation="MSSDADAHKVGLIPVILMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            4957..6600
                     /gene="eptA"
                     /locus_tag="JNZ05_001878"
     CDS             4957..6600
                     /gene="eptA"
                     /locus_tag="JNZ05_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HAY8565128.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRTLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            6597..7265
                     /gene="basR"
                     /locus_tag="JNZ05_001879"
     CDS             6597..7265
                     /gene="basR"
                     /locus_tag="JNZ05_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HAY8565129.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLMVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            7266..8366
                     /gene="pmrB"
                     /locus_tag="JNZ05_001880"
     CDS             7266..8366
                     /gene="pmrB"
                     /locus_tag="JNZ05_001880"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HAY8565130.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(8342..8431)
                     /gene="pmrR"
                     /locus_tag="JNZ05_001881"
     CDS             complement(8342..8431)
                     /gene="pmrR"
                     /locus_tag="JNZ05_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HAY8565131.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(8543..10045)
                     /gene="proP"
                     /locus_tag="JNZ05_001882"
     CDS             complement(8543..10045)
                     /gene="proP"
                     /locus_tag="JNZ05_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HAY8565132.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(10309..11187)
                     /locus_tag="JNZ05_001883"
     CDS             complement(10309..11187)
                     /locus_tag="JNZ05_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565133.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(11184..13412)
                     /gene="crfC"
                     /locus_tag="JNZ05_001884"
     CDS             complement(11184..13412)
                     /gene="crfC"
                     /locus_tag="JNZ05_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HAY8565134.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            13814..14149
                     /locus_tag="JNZ05_001885"
     CDS             13814..14149
                     /locus_tag="JNZ05_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HAY8565135.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            14307..14750
                     /gene="yjdN"
                     /locus_tag="JNZ05_001886"
     CDS             14307..14750
                     /gene="yjdN"
                     /locus_tag="JNZ05_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HAY8565136.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            14883..15671
                     /gene="phnC"
                     /locus_tag="JNZ05_001887"
     CDS             14883..15671
                     /gene="phnC"
                     /locus_tag="JNZ05_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAY8565137.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            15696..16712
                     /gene="phnD"
                     /locus_tag="JNZ05_001888"
     CDS             15696..16712
                     /gene="phnD"
                     /locus_tag="JNZ05_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8565138.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            16818..17597
                     /gene="phnE"
                     /locus_tag="JNZ05_001889"
     CDS             16818..17597
                     /gene="phnE"
                     /locus_tag="JNZ05_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAY8565139.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAEEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            17618..18343
                     /gene="phnF"
                     /locus_tag="JNZ05_001890"
     CDS             17618..18343
                     /gene="phnF"
                     /locus_tag="JNZ05_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HAY8565140.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            18344..18796
                     /gene="phnG"
                     /locus_tag="JNZ05_001891"
     CDS             18344..18796
                     /gene="phnG"
                     /locus_tag="JNZ05_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HAY8565141.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHTERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            18793..19377
                     /gene="phnH"
                     /locus_tag="JNZ05_001892"
     CDS             18793..19377
                     /gene="phnH"
                     /locus_tag="JNZ05_001892"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HAY8565142.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAISEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDDGIS
                     SKQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            19377..20441
                     /gene="phnI"
                     /locus_tag="JNZ05_001893"
     CDS             19377..20441
                     /gene="phnI"
                     /locus_tag="JNZ05_001893"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HAY8565143.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGVADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLVHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            20434..21279
                     /gene="phnJ"
                     /locus_tag="JNZ05_001894"
     CDS             20434..21279
                     /gene="phnJ"
                     /locus_tag="JNZ05_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HAY8565144.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            21276..22034
                     /gene="phnK"
                     /locus_tag="JNZ05_001895"
     CDS             21276..22034
                     /gene="phnK"
                     /locus_tag="JNZ05_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HAY8565145.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            22145..22825
                     /gene="phnL"
                     /locus_tag="JNZ05_001896"
     CDS             22145..22825
                     /gene="phnL"
                     /locus_tag="JNZ05_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HAY8565146.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            22822..23958
                     /gene="phnM"
                     /locus_tag="JNZ05_001897"
     CDS             22822..23958
                     /gene="phnM"
                     /locus_tag="JNZ05_001897"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HAY8565147.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLLLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHLLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     SGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTQN
                     PAQALNLQDRGVIGEGKRADLVLAHRKDNHIHIDHVWRQGKRVF"
     gene            23958..24515
                     /gene="phnN"
                     /locus_tag="JNZ05_001898"
     CDS             23958..24515
                     /gene="phnN"
                     /locus_tag="JNZ05_001898"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HAY8565148.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAEIRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANSLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQALARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            24502..24936
                     /gene="phnO"
                     /locus_tag="JNZ05_001899"
     CDS             24502..24936
                     /gene="phnO"
                     /locus_tag="JNZ05_001899"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HAY8565149.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVSMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTALSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            24938..25696
                     /gene="phnP"
                     /locus_tag="JNZ05_001900"
                     /pseudo
     CDS             24938..25696
                     /gene="phnP"
                     /locus_tag="JNZ05_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
     gene            complement(25843..26172)
                     /locus_tag="JNZ05_001901"
     CDS             complement(25843..26172)
                     /locus_tag="JNZ05_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565150.1"
                     /translation="MKPYSTLFLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNSDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRCQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(26241..26873)
                     /locus_tag="JNZ05_001902"
     CDS             complement(26241..26873)
                     /locus_tag="JNZ05_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY8565151.1"
                     /translation="MIVMPTTYSPMTVARSFRVVEEIELSGRLLQVIYDNQTPRVAII
                     EADIEVFAGVPRHRVLAALDLQRKKEFGSDIIAVERWWEDSDLVQVEGICVSPGERDK
                     GLATRLYEALILQCGITLISDFDQYDGGKMLWQKIARESDQISVFVLDTEQGAFWPYD
                     GTKVIYDGGCIPEERIWSISPDQKCHGIVLVAENKQRANQLMEESAPYRI"
     gene            complement(26954..29169)
                     /locus_tag="JNZ05_001903"
                     /pseudo
     CDS             complement(26954..29169)
                     /locus_tag="JNZ05_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
BASE COUNT         6849 a         8279 c         7671 g         6524 t
ORIGIN      
        1 ttccttcata accgggtaag caatgatgaa agtattaatt gttgaaagcg agtttctcca
       61 tcaagacacc tgggtcggta acgccgttga gcgtctggca gatgctttaa gccagcaaaa
      121 tgttaccgtg attaaatcca cctcctttga tgatggtttt gccattctct cttcaaacga
      181 agccattgac tgcctgatgt tcagctatca aatggaacat ccggacgaac atcaaaacgt
      241 cagacaattg atcggtaagc ttcatgagcg ccaacaaaac gtgccggtct tcctgttggg
      301 cgatcgggaa aaagccctcg ccgcaatgga ccgggatctg ctggagcttg tcgatgaatt
      361 cgcctggatt ctggaagata ccgccgactt tatcgccgga cgcgccgttg ccgcgatgac
      421 ccgctaccgc cagcagctgt tgccgccact gttcagcgcg ctgatgaaat atagtgacat
      481 ccatgaatat tcctgggcag cgccaggcca ccagggcggc gttggtttta ccaaaacacc
      541 cgccggacgt ttctaccatg actactatgg tgaaaatctg ttccgcaccg acatgggcat
      601 cgaacgaact tccctcggtt ctttgcttga ccatactggc gcatttggca aaagcgaaaa
      661 atatgccgca cgcgtatttg gtgccgatcg ctcctggtcg gtagtcgtcg gtacttccgg
      721 ctctaaccgc accatcatgc aggcttgcat gaccgataac gatgtcgtgg tcgttgaccg
      781 taactgccat aaatccatcg aacaaggttt gatgctgaca ggcgcgaaac cggtctatat
      841 ggtgccaagc cgcaaccgct acggcattat cgggccaatc tatccgcagg aaatgcaacc
      901 tgaaaccttg caaaagaaaa tcagtgaaag cccgctgacc aaagacaaag ccgggcaaaa
      961 accgtcttac tgcgtggtaa ccaactgcac ctatgacggc gtgtgttata acgctaaaga
     1021 agcgcaggat ctgctggaaa aaacctccga tcgtctgcac tttgacgaag cctggtacgg
     1081 ctatgcacgt ttcaacccga tctatgccga tcactatgcc atgcgcggcg aacctggcga
     1141 tcacaacggt cctaccgttt tcgccaccca ctccatccac aaactgctga atgcgctgtc
     1201 acaggcttct tatattcatg tacgtgaagg tcgtggggcg attaacttct cccgcttcaa
     1261 ccaggcctac atgatgcatg ccaccacctc cccgctgtat gccatctgcg catccaacga
     1321 cgtggcggtg tcgatgatgg acggcaacag cggcctgtca ctgacacagg aagtgattaa
     1381 cgaagcggtt gatttccgtc aggcgatggc gcggctatat aaagagttca ccgctgacgg
     1441 tagctggttc ttcaaaccgt ggaacaaaga agtcgtcacc gacccacaaa ccggcaaaac
     1501 ctatgacttt gctgacgcac caaccaaact gctgaccacc gttcaggact gctgggtaat
     1561 acatccgggc gaaagctggc acggcttcaa agatattccg gataactgga gtatgctcga
     1621 cccaattaaa gtcagcatcc ttgctccggg aatgggtgaa gatggtgaac tggaagaaac
     1681 cggtgttccg gcggcgctgg tcactgcctg gcttggtcgc cacggcattg tgcctacccg
     1741 caccactgac ttccaaatta tgttcctgtt ctctatgggc gtaacccgtg ggaaatgggg
     1801 gactctggtt aacacccttt gctccttcaa acgccactat gacgccaaca caccgctggc
     1861 gcaggtgatg ccggaacttg ttgaacaata tcctgacact tacgcgaaca tggggattca
     1921 cgatctgggt gacaccatgt ttgcctggct gaaagaaaac aaccctggcg cacggttgaa
     1981 cgaagcctat tccggcctgc cggtggcgga aatcaccccg cgtgaagcgt acaacgcgat
     2041 tgtcgacaac aatgtcgaac tggtatccat tgaaaatctg ccaggacgca tcgcggcaaa
     2101 ctcagttatc ccgtatccgc caggaatccc gatgctgctg tctggtgaaa acttcggcga
     2161 taaaaacagt ccgcaagtaa gttatttacg ctcgctgcaa tcctgggacc accatttccc
     2221 tggatttgaa cacgaaactg aagggactga aattattgac ggtatttacc acgttatgtg
     2281 cgtgaaagcg taaccactat tccgctgaag gcgtaattgt ttaaataaca ttacgccgcc
     2341 tggccttagg ccttttgagt atggcaacgt tttcataaaa attgctgcaa acaaaaatgt
     2401 catacttttt gcgcggcccc accccgcgct tttttttgcc tgttatttat cctgtaaaaa
     2461 aatatgtaca tgagaaatta ctataaaaat ttgtaatatt agtaaaactc gttattttta
     2521 tgcatgttta tattcatcat acaattatat aaccatttcc cggtatcgct ttgctttagc
     2581 gagaactggt cttttattcg cactcaggag tacatgtatg aggatttgca gcgaccaacc
     2641 ttgtattgtt ttattgactg aaaaagatgt ctggataagg gtgaatggga aagaacctat
     2701 tagccttaaa gctaaccata tggcgttatt aaattgtgaa aataatatta tcgacgtctc
     2761 ctctcttaac aacactttgg ttgctcatat tagtcacgac atcatcaaag attacctccg
     2821 gtttctgaat aaagatctct cgcaaatacc agtatggcaa cgtagcgcta cgcccatact
     2881 caccctgcca tgcctgacgc cagacgtctt tcgcgttgcc gcgcaacaca gcatgatgcc
     2941 cgcagaaact gagtcagaaa aggaacgaac acgcgcatta ttattcacgg tgctatcccg
     3001 ttttctcgac agtaaaaaat tcctttcatt aatgatgtat atgttacgta attgtgtaag
     3061 tgacagcgtt tatcaaatta ttgaaagcga tattcacaaa gactggaatc ttagtatggt
     3121 agccagttgt ttatgtctta gcccaagtct gttaaagaaa aagttgaaaa gcgaaaacac
     3181 cagttatagc caaataatca ccacctgccg catgcgttat gccgtaaatg aattaatgat
     3241 ggacggtaaa aatatctctc aggtatcaca gtcctgcggc tacaacagta cgtcgtactt
     3301 tatttctgtc tttaaagact tctacggtat gacgccgctg cattatgtta gtcagcacag
     3361 agaacgcact gtcgcctgat ttttaacctt aacgaagagc tatattaata acggcatcag
     3421 cgataacccg gtcgataata attcaactat cgaatgcagg cgtatgatat gacgtaattt
     3481 attgtcacga agctcgcctt cgcaggagtt taattatgtc ttcggatgct gatgctcaca
     3541 aagtgggctt aatccccgtc atcctgatgg tgtcggggaa tattatgggg tcaggtgttt
     3601 ttctgttacc tgcaaacctg gcctccactg gcgggattgc catttatgga tggttggtga
     3661 cgattatcgg tgcgctgggg ctctcgatgg tatacgccaa aatgtcgttc ctcgacccaa
     3721 gtcctggtgg ttcttacgct tacgcccgcc gctgctttgg cccgtttctc ggttatcaaa
     3781 ccaacgtcct ctactggctg gcctgctgga tcggcaatat cgccatggtg gtcattggcg
     3841 taggatattt aagttacttc ttcccgattc tgaaagatcc attggtatta accatcacct
     3901 gcgtcgtggt gctgtggatc ttcgtcctgc tgaacattgt cggtccgaaa atgatcaccc
     3961 gcgtgcaggc cgttgccact gtgctggcgc tgatccccat cgtcgggatt gccgtatttg
     4021 gctggttctg gttccgtggt gaaacctata tggcggcatg gaacgtcagc ggcctgggca
     4081 ccttcggtgc gattcaaagt acccttaacg ttacgctgtg gtcgttcatc ggtgtggaaa
     4141 gtgcctccgt tgccgcaggt gtggtgaaaa acccgaaacg caatgtccct atcgccacca
     4201 ttggtggggt attgattgcc gccgtttgct atgtgctctc caccaccgcg attatgggga
     4261 tgatccctaa tgccgcactg cgcgtttctg cctcgccatt cggtgatgcc gcacggatgg
     4321 cgttgggtga caccgctggg gccattgttt ccttctgcgc agctgcgggt tgcttaggtt
     4381 cactgggcgg ctggacgttg ctggcgggtc aaacggcgaa agccgctgcc gatgacggac
     4441 tgttcccacc gatttttgcc cgtgtaaata aagcgggtac gccagtggcg gggctgatta
     4501 tcgtcggtat tttgatgacc atcttccagc tcagcagcat ttcaccaaac gcgaccaaag
     4561 agttcggtct ggtttcttcc gtctcggtca tctttacact ggtgccatat ctttacacct
     4621 gtgcggcgtt actgctgctc ggccacggtc actttggtaa agcacgcccg gcatatctgg
     4681 ctgttactac cattgccttc ctctactgca tttgggccgt ggtggggtcc ggggcgaaag
     4741 aggttatgtg gtcatttgtc accctgatgg tcatcaccgc catgtatgcg ctgaattaca
     4801 accggctaca taaaaacccg tatcccttag atgcaccaat aagcaaagat taattcccct
     4861 taatccagca aacataaaag ccaaccttaa gaacttaagg ttggcttaat tttgctttgc
     4921 gagcatatgc gcactttgtt cgatggaaac accgtgatgt tgaagcgcct actaaaaaga
     4981 ccctctttga atttactcgc ctggctattg ttggccgctt tttatatctc tatctgcctg
     5041 aatattgcct tttttaaaca ggtgttgcag gcgctgccgc tggattcgct gcataacgta
     5101 ctggttttct tgtcgatgcc ggtcgtcgct ttcagcgtga ttaatattgt cctgacacta
     5161 agctctttct tatggcttaa tcgaacactg gcctgcctgt ttattctggt tggcgcggct
     5221 gcacaatatt tcataatgac ttacggcatc gtcatcgacc gctcgatgat tgccaatatt
     5281 attgatacca ctccagcaga aagttatgcg ctgatgacac cgcaaatgtt attaacgctg
     5341 ggattcagcg gcgtgcttgc tgcgctgatt gcctgctggc taaaaatcaa acctgccacc
     5401 tcgcgtctgc gcagtgttct tttccgtgga gccaatattc tggtttctgt actgctgatt
     5461 ttgctggtcg ccgcactgtt ttataaagac tacgcctcgt tgttccgcaa taacaaagag
     5521 ctggtgaaat ccttaagccc ctctaacagc attgttgcca gctggtcatg gtactcccat
     5581 cagcgactgg caaatctgcc gctggtgcga attggtgaag acgcgcaccg caacccgtta
     5641 atgcagaacg aaaaacgtaa aaatttgacc atcctgattg tcggcgaaac ctcgcgggcg
     5701 gagaacttct ccctcaacgg ctacccgcgt gaaactaacc cgcggctggc gaaagataac
     5761 gtggtctatt tccctaatac cgcatcttgc ggcacggcaa cggcagtttc agtaccgtgc
     5821 atgttctcgg atatgccgcg tgagcactac aaagaagagc tggcacagca ccaggaaggc
     5881 gtgctggata tcattcagcg agcgggcatc aacgtgctgt ggaatgacaa cgatggcggc
     5941 tgtaaaggtg cctgcgaccg cgtgcctcac cagaacgtca ccgcgctgaa tctgcctgat
     6001 cagtgcatca acggcgaatg ctatgacgaa gtgctgttcc acgggctgga agagtacatc
     6061 aataacctgc agggtgatgg cgtgattgtc ttacacacca tcggcagcca cggtccgacc
     6121 tattacaacc gctatccgcc gcagttcagg aaatttaccc caacctgcga caccaatgag
     6181 atccagacct gtaccaaaga gcaactggtg aacacttacg acaacacgct ggtttacgtc
     6241 gactatattg ttgataaagc gattaatctg cttaaagaac atcaggataa atttaccacc
     6301 agcctggttt atctttctga ccacggtgaa tcgttaggtg aaaatggcat ctatctgcac
     6361 ggtctgcctt atgccatcgc cccggatagc caaaaacagg tgccaatgct gctgtggctg
     6421 tcggaggatt atcaaaagcg gtatcaggtt gaccagaact gcctgcaaaa acaggcgcaa
     6481 acgcaacact attcacaaga caatttattc tccacgctat tgggattaac tggcgttgag
     6541 acgaagtatt accaggccgc ggatgatatt ctgcaaactt gcaggagagt gagtaaatga
     6601 aaattctgat tgttgaagac gatacgctgt tattgcaggg actgattctg gcggcgcaaa
     6661 ccgaaggcta cgcgtgcgat ggcgtgacaa ccgcgcggat ggcggaacaa agccttgagg
     6721 acggtcatta cagcctgatg gtactggatt tagggttacc cgacgaagat ggactgcatt
     6781 ttctcgcccg tatccggcag aaaaaataca ccctgccggt actgatcctc accgctcgcg
     6841 atacgctgac cgacaaaatc gccgggctgg atgtcggtgc cgatgactat ctggtgaagc
     6901 cttttgcgct ggaagagtta cacgcccgta tccgcgccct gctacgacgc cataataatc
     6961 agggcgaaag tgagctgatt gttggcaatc tgacgctgaa tatgggtcgc cgtcaggtat
     7021 ggatgggcgg tgaagagttg attctgacgc ccaaagaata tgctctgctg tcacggttaa
     7081 tgctcaaagc aggcagtccg gtacatcggg aaattctcta caacgacatc tataactggg
     7141 acaatgaacc ctcgaccaac accctggaag tgcatatcca caatctgcgc gacaaagtgg
     7201 gcaaagcccg tatccgcacc gtgcgcggct ttggctatat gctggtcgcg aatgaggaaa
     7261 actaattgaa tctgatgcgt tttctgcgcc gaccaatatc gctgcgccaa cggctgatat
     7321 tgaccatcgg ggccattttg ttggtgtttg agctgatcag cgtcttctgg ctatggcatg
     7381 aaagtaccga gcagattcaa ctgtttgagc aggcgctacg cgacaatcgc aacaacgatc
     7441 gccacatcat gcgtgagatc cgcgaggcgg tcgccagcct gattgtcccc ggcgtcttta
     7501 tggtcagcct gacgctattt atctgctatc aggcggtacg ccgcatcacc cgcccgctgg
     7561 cggagctgca aaaagagctg gaagcgcgca ccgccgacaa cttaacgccc attgccattc
     7621 acagcgccac cctcgaaatc gaagcggtgg tttcggcgtt gaacgatctg gtcagtcgcc
     7681 tgaccagcac gctggataac gaaaggttgt ttaccgctga cgtcgcgcac gaactgcgaa
     7741 cgccactggc gggggtgcgt ttgcatctgg aactgctggc gaaaacgcat cacattgatg
     7801 tagcaccgtt agtggcacgg cttgatcaga tgatggagag cgtctcccag ctgctgcaac
     7861 tggcgcgtgc cggacagtca ttttcttccg gtaattatca acatgtaaaa ctgctggaag
     7921 atgtcattct cccctcgtat gacgaactca gtaccatgct cgaccagcga cagcaaaccc
     7981 tgctattgcc agagagcgcc gccgacatca ccgttcaggg tgatgcaacc ctgctgcgga
     8041 tgttattgcg aaacctggta gaaaacgccc atcgttacag cccgcaaggc agcaacatta
     8101 tgattaagct gcaagaagat ggcggagcgg tcatggcagt tgaagatgaa ggaccaggta
     8161 ttgatgagag taaatgcggg gagttgagta aagcgtttgt acgtatggac agccgttatg
     8221 gcgggattgg tctggggtta agtattgtca gccgcattac acagttgcat cacgggcagt
     8281 ttttcctgca aaaccggcaa gagacttccg gcacgcgggc ctgggtacgg ctgaagaaag
     8341 atcagaacgt ggcaaaccag atataaagaa agctgctgac caccagcacg ctgaacacgg
     8401 tagttaaact ttcataaaca cggtttttca ttgcactctc ctccgttaac ctggaggaga
     8461 gtatgcgcgt cagagattaa gtgaacctta agagttcaat tggcctgata agacagcgtc
     8521 acatcaggcc atccgtttca gcttattcat caattcgcgg atgttgctgc accaggcggg
     8581 tacgtttcgc ctgcaaatcg gcaatctcgt ggtcaatatt atcgattttc tgctcgatat
     8641 tatcgtaatg ctcgacgaga atttccttcg cttcctgtat atctgacgcc gccggtgtcg
     8701 cacctttcaa cggacgattt gccgtctctt tcatggttac gccggtgatt aaaccaatca
     8761 ccgccactac catcaggtaa taggcaggca tcatcagatt ctgcgagctt tcgaccagcc
     8821 aggccgccag tgttggcgtc agaccggcaa ccagcaccga aatattaaat gccgccgcca
     8881 gcgcgctgta acggatatgc gtcgggaaca tcgctggcaa ggtagaagcc ataacgccca
     8941 taaagcagtt aaggatcacc gccagcatca gtaacccggc aaaaatcagg ccgatgacgt
     9001 tactgttaat cagaataaac gccgggatcg ccaacacaaa cagtgcaaca ctaccaagta
     9061 gcacaaacgg acgacggcca aaacggtcac tcagcaagcc catcaccggc tggacaaaca
     9121 gcataccgat cataatggcg ataataatca gcaccccgtg gtcttcggag taatgcaggt
     9181 tatgcgacaa ataactcggc atataggtca gcaacatgta gtaagtcacg ttggtggcaa
     9241 ttaccagacc aatacatgtc aacaggctgc gccagtattt agtggcaatc tctttaaacg
     9301 agactttcgg gccatcctgc aaaccttcgc ggtcgccctg ttccagttta tcaacatgct
     9361 gctggaacgc cggagtttct tccagcgcat ggcgcaggta aagcccgata atccctaacg
     9421 gcagagcaat aaagaacgga atacgccagc cccagtcgag gaagttcgct tcgccgacaa
     9481 tggtcgaaat taacaccacc acgcccgcac ccagcacaaa cccggcaata gaaccgaagt
     9541 ccagccagct gcccataaag ccacgtttac ggtcggggga gtattccgca acaaatatcg
     9601 acgccccggt atattcacca ccgaccgaga aaccttgtgc catcttacag atcaacagca
     9661 gaatcggtgc ccaaatacca atcgtgtcgt aggacggtat taagccaata cagaacgtac
     9721 tgatcgacat aatcacaata gtgatagcga ggatcttctg gcgaccatat ttatcgccca
     9781 acataccaaa gaagagtccg ccaagcggtc gaatcagaaa gggaacggag aaagtggcaa
     9841 gtgcagcaac catctgcacg ctggggtcag cccccgggaa aaaaacttta cctaatgcgt
     9901 aagcaacaaa accataaaca ccaaaatcaa accattccat tgcattaccc agtgatgctg
     9961 cggtaatggc tttacgcagt ttaccgtcat caataatggt gacatcacga agggtaatcg
    10021 gttttacttt tttccttttc agcatagctt tcctcgcaga gttgacccat ttccgcaaac
    10081 gccagaaaaa tcagcgcgac ggccgcaaaa ggggagcggg aattgcagga tgcaatctct
    10141 gtaacaagcg tagcgggttt gcttacaccc tccggttaag gaatgtacaa tcaaaccagt
    10201 tgacgcctga attgggcagt taatgacctt tttaccctac cagtgtttaa atttgtgatc
    10261 aacttcacac atggatttcg tttaggactc attgatgtaa ctgattattt acgccaaatt
    10321 tacagagaag atttcctcga ccattgcccc caccaaacgc tgcgctgaac agagtcgtgg
    10381 catccccagc gcgacagttt gccagcgttg ggtcactgac cagcaaacgg gttgtttatc
    10441 tgccgtggtc cactttccca gccaacttaa aaaatcagca tgatccccgc ccgtcggaac
    10501 ggtcgaagag gcaaaccact gatggtaata gtgacgcgtg gtcggtgccg cattgatact
    10561 ctctgccaga tgttgtgtcg ctttctcgcg caaatcaccc agcaacaaca ccaccgcttg
    10621 cgggcttccc agacggcagg catcgccaaa tgcaccccag cgcaactcct ccagaacgat
    10681 aaaacagcgc ccaggcaacg accaggcgtt atacgcccca gcacgccagc gggtaaaaat
    10741 ttgctcgctc tgatcacctg cctgcaccag taggcctcgt tgcgttaacg ccttttcctt
    10801 ccagcgacaa cgggctgaaa accactcata caaggcggca atttgctggg ggagctccgg
    10861 gaaagcagag tgctggcggt gatccatttt ttgcagcagc gccagatgct gcgcgatctg
    10921 cgtcagcgcc agatgaccgg gatccagcat cgacgccagt ttttccacca ttgtcagtcg
    10981 cagactaagc ggcgcattta acatgccgcc gttcgtccat gtctgaagct gcacacgcga
    11041 aagaagttcc tgcatcagtc gttcacgaaa ctgctgctga tgggcttgag gatggcgggc
    11101 atcatcgccc tgcgccagat ccactaaaaa gcgcggatga accgactcca gcacgcgacc
    11161 ggggccgtcc agtaacgtct tggtcaatat cgttctgccg tgaaaagtgt ttgaatatca
    11221 tcgcgtaaca gctgagcgtc ggtgtaaatc cagctgtgag tcatcacagt ctgctgcaat
    11281 tgctgctgca tcagcctgac caccgattca ttttgttgac gcagagccag actttcgcgt
    11341 aaacgcgtct gtaattctgt caaacatgaa gcgaactcag cgaaaaaagt attcatgcct
    11401 gccgtaacag attcatcgac ctgctctgcc agaactttag ccatttgttg gcaatagaga
    11461 tcgacttctg cgcttaatgc tcgttgcaac acactgtaat caaccgtttc tgtcggggat
    11521 ttctcatttc cccgtcccca gtcgggctga ttcaaccagc gcgaaaaagt ctcacgcaca
    11581 acgcctaaac gcgtgctctg ctcgtccgtt gcttcctggc gcgaaatgac ttcactgaac
    11641 agctggcgag tgttgaagtg gggaactacg ccgtgaaaaa caggaaaatg aaacccagga
    11701 cgaaaccctg actcgctcaa ttccattttg acttgttgct caatggggcg aataacatcg
    11761 gttaacaccc ggcaaagggt ggattccagc tcggcaaaac gcagcgtaaa gtcgcgactg
    11821 atggtgttct gcgccgtctg taacagtgtc tcacagcggg tacgaatctc gcttaacggc
    11881 tccgaatcat cctgaaacaa ggcggctaac tgcgcattca ccgcatcttg ctgttgacgc
    11941 agaaagtggt tggcggaggt cagggccagc tcgatttcat gtttaatctc gccgctcacc
    12001 tgcgcctgat tgagttgcaa tagctgcaaa ctttcttcga cctgatggat attttgccgc
    12061 aattgttcac aagcgacgtt taacccgtgc gcacgaaaat ccaggtattc ccgcgcctgc
    12121 tgcgcgtaat tcaacagttt atgcgcagca gatcgcagag catacaatga ggcgttagcg
    12181 taagcggcat gaagcaacgc ctgaattggc tgggcgaaca gcgaatcttc ccacaactga
    12241 tcggcagcat gacgaatatg ttcgaggtcc gccagatcgg catgacgcca gcgcctgccg
    12301 agcgcggcat gggcaaaatc ttccacccag cgttgttgct ctggcgctgg taacttaccg
    12361 ttgttggcta actcatagcg cgcccgattc gccaggtagc cccacatcga cgacaccggg
    12421 aatatctgct gtggcgtaat acagcctttc atcagcgtcc cggaaatcag tgcccgcacc
    12481 tggtcggcgt cgtcactgtt acgatcctgt tgatcgaact tattgaccag cacatacagc
    12541 ggcaccgatt gccccaccgc caaaatcgcc tcacggacct cttcatcgga gatcgatttc
    12601 agttgcgtat aatccagcac cgccagtacc gccgaggcgc gtgccagctg ctggttaagc
    12661 attttttgca gatgcggttg cccggcttca tttggcccgg gggtatccag taacgtcaac
    12721 tgaccgggat aactttccag ccccgccaga tggacaaact ccacttcaat cacgggaata
    12781 tgctcaatgg cggcgtaagc agaaaaagga aaatcgacgt ccagcgcctt cgccagtcgc
    12841 actaaatcat tcaaactttt cagacaatga aaaataggct gggcacccag ataatatttt
    12901 tcgaaagcga cgccattttc gatccgctgc ataagcgcac gcatatcttt atctatttcc
    12961 agcacatcgg tcagatgctt aatatcgcaa tcacgcaggc gctgttgtaa ttgttgaatt
    13021 aaacaatcga ttggcgcgac atgtgaaaaa tgcagtaccg gttccttttg cccgggcgta
    13081 tggcgaataa gcgtcggcag cgcagtcatt gggcgattac gattaggcag aacctccgta
    13141 ccaacaatgg cattaatggt ggttgatttc cctgctttca tggtaccgac aattgccagc
    13201 accatttcca gtcgggaaat tttacgcaac tcattattca gcatcgcgtg acgttcggcg
    13261 atattaggct gactccaggg taaagccagt tgtggcgcgt cgtctccggg tacagagaga
    13321 ggcatttttt ccagtaactg caactgttgg cgagaaagct gtaacaggcg ttcagcctcc
    13381 tgacttaact catacagggt ctgtgtgtac atagaaaatt cttccttaaa gcaaattttg
    13441 ttacttttat ttagccagat tgtttttgag ttctgttttc ggcttttata attactgcaa
    13501 gaaataattt tatatttagt gtgttgtttt ttatcagaat aaataacgtc ttctgatgcg
    13561 tttaaaacgt cagaaagata aaaatatcat gtaaataaaa aaaagaacaa gtagagcatt
    13621 aacattatct taaataataa atagaggcaa aaagattatt ttctttttgc gtttcctttc
    13681 aaatgaaaac gatcgtcgtc taaaatcagc agtacccccg acaaactcag ggattttgtg
    13741 tataattgcg gcctttttcg gcaatctgcc gttttttggc gcttttgccc tgctgacttt
    13801 tgaggaaatc cacatgtcat taccacactg cccaaaatgc aactccgaat acacttacga
    13861 agataacggc atgtacatct gcccggaatg tgcctacgaa tggaacgacg cagaacctgc
    13921 acaggaaagc gacgagctga tcgttaaaga tgctaacggc aatctgctgg ctgacggcga
    13981 cagcgttacc atcattaaag atctgaaggt gaaaggtagc tcttcgatgc tgaaaattgg
    14041 caccaaagtg aaaaacatcc gcctggttga aggcgaccat aacatcgatt gcaaaatcga
    14101 cggttttggt ccgatgaaac tgaaatctga gtttgtgaaa aagaactgat tgtattgtga
    14161 tcggtaagcc gaataaggcg ctcgcgccgc atccggcaac gatgccgact gcctgatgcg
    14221 acgcttgcgc gtcttatcag gcagcctctc atctgtataa atttcgaact acacttaact
    14281 ggcttctctt aactgaggtc accatcatgc cgttaagtcc ctacctctct tttgccggta
    14341 actgttccga cgcgattgcc tattatcaac gtacgttggg cgcggaactg ctctataaaa
    14401 tcagcttcgg cgaaatgccg aaatcagcgc aggacagcgc cgagaactgc ccttccggaa
    14461 tgcaatttcc cgataccgcc atcgctcatg ccaacgtgcg cattgccgga agcgacatca
    14521 tgatgagcga tgctataccg tcaggaaaag ccagctactc cggctttacg ctggtgctcg
    14581 attcgcaaca ggtcgaagaa ggaaaacgct ggtttgataa ccttgccgct aacggaaaaa
    14641 tcgaaatggc ctggcaggaa actttctggg cgcatggctt tggcaaagtc accgataaat
    14701 ttggcgtacc gtggatgatt aatgtcgtca aacaacaacc aacgcaataa cccgccggga
    14761 ggcccgccct cccgcactgt catcgaattc ccgttaactc ttcatctgtt agtcactttt
    14821 aattaaccaa atcgtcacaa taatccgcca cgatggagcc acttttttag ggaggctgca
    14881 tcatgcaaac gattatccgt gtcgagaagc tcgccaaaac cttcaatcag catcaagcgc
    14941 tgcatgcggt tgatctgaac attcatcaca gtgaaatggt ggctctgctt gggccgtcgg
    15001 gttccggcaa atccaccctt ttacgtcatt taagcggttt gattaccggc gataaatccg
    15061 ccggtagcca tatcgagcta ctgggccgca ctgtccagcg cgaaggccgc ctggcccgcg
    15121 atatccgcaa aagccgcgcc aacaccggct acatctttca acaattcaac ctggtgaacc
    15181 gcctgagcgt actggagaac gtgctgattg gcgcgctcgg cagcacgccg ttctggcgca
    15241 cctgttttag ctggttcacc ggcgagcaga aacagcgcgc gttacaggcg ctgacccgcg
    15301 ttggcatggt gcattttgcc catcagcgcg tttccaccct ctccggcggc cagcagcaac
    15361 gtgtggcgat tgcccgtgcg ctgatgcagc aggcgaaagt gattctggcc gatgaaccca
    15421 tcgcctcgct ggacccggaa tcagcgcgca tcgtgatgga caccctgcgc gacatcaacc
    15481 agaacgacgg catcaccgtg gtcgtcacgc tgcatcaggt ggattacgcc ctgcgctact
    15541 gcgaacgcat cgtcgccctg cgtcaggggc acgtcttcta cgacggcagc agccaactgt
    15601 ttgataacga acgttttgac catctctacc gcagcattaa ccgcatcgaa gagaacgcga
    15661 aagctgcctg atatccccat cattgaggaa aacgaatgaa cgctaagata attgcctcgc
    15721 tggccttcac cagcatgttc agcctcagca ccctgttaac cccggcacac gccgaagagc
    15781 aggaaaaggc gctgaatttc ggcattattt caacggaatc acagcaaaac ctgaaaccgc
    15841 aatggacgcc gtttttacag gatatggaga agaaactggg cgtgaaggtc aacgccttct
    15901 ttgccccgga ctacgcgggc attatccagg ggatgcgctt caataaagtg gatattgcct
    15961 ggtacggcaa cctgtcggcg atggaagcgg tggatcgcgc caatggccag gtcttcgccc
    16021 agacggtcgc ggcggatgga tcgccgggtt actggagcgt gttgatcgtc aacaaagaca
    16081 gcccgatcaa caacctgaac gatctgctgg cgaagcggaa agatctcacc ttcggcaatg
    16141 gcgatcctaa ctccacctct ggcttcctcg tccccggtta ctacgtcttc gctaaaaaca
    16201 atatctccgc cagcgacttc aagcgcaccg tcaacgccgg gcatgaaacc aacgcgctgg
    16261 ccgtcgccaa caagcaggtg gatgttgcca ccaacaacac cgaaaacctc gacaagctga
    16321 aaacctccgc gccggagaag cttaaagagc tgaaggtgat ctggaaatcg ccgctgatcc
    16381 taggcgatcc gatcgtctgg cgcaagaatc tgtccgaaac taccaaagac aagatctacg
    16441 acttctttat gaactacgga aaaacgccgg aagagaaagc ggtgctggaa cgcctgggct
    16501 gggcgccgtt ccgcgcttcc agcgacctgc aactggtgcc gattcgccag ctcgcactgt
    16561 ttaaagagat acaaagcgtg aaaggcaata aagggctgaa tgagcaggac aagctggcga
    16621 aaaccaccga gattcaggcg cagctggatg acctggaccg cctgaacaac gcgttaagcg
    16681 cgatgagttc ggtgagtaaa gcggtgcagt aaatcgtagg tcggataaga cgccccggcg
    16741 tcgcatccga caatgtgcag gcgttgatgc cggatgcggt gcaagcacct tatccggcct
    16801 acagaccgga gccaaacatg caaaccatca ccatcgcccc acccaagcgt agctggttct
    16861 cgcttctgag ctgggccgtt gttctcgccg tgctggtcgt ctcgtggcag ggcgcggaaa
    16921 tggccccgct tacgctgatt aaagacggcg gcaacatggc aaccttcgct gccgacttct
    16981 tcccgcccga tttcagccag tggcaggatt acctcaccga aatggccgtc acgctgcaaa
    17041 tcgccgtctg gggcaccgcg ctggcagtgg ttctctccat ccccttcggt ctgatgagcg
    17101 ccgaaaactt ggtgccgtgg tgggtttacc agcccgttcg ccgcctgatg gacgcctgcc
    17161 gcgccattaa cgaaatggtc ttcgccatgc tgttcgtggt cgccgtcggc ctcggcccgt
    17221 tcgctggcgt gctggcgctg tttatccaca ccaccggcgt gctctccaag ctgctttccg
    17281 aagcggagga agcgatcgaa cccggcccgg tggaaggcat tcgcgccacc ggcgccaaca
    17341 aactcgaaga gatcctttac ggcgtgctgc cgcaggtgat gccgctgctg atctcctact
    17401 ccctctatcg cttcgaatcc aacgtccgct cggcgaccgt cgtcggcatg gtcggcgcgg
    17461 gcgggatcgg cgtcaccctg tgggaagcga ttcgcggttt ccagttccag caaacctgcg
    17521 ccctgatggt gcttatcatc gtcacggtca gcctgctgga tttcctctct caacggttgc
    17581 gtaagcactt tatctgataa gcgaggcatt gatatctatg cacttgtcta cacatccgac
    17641 cagctaccca acacgctatc aggagatagc cgcaaaactt gagcaggagc ttcgtcaaca
    17701 ctaccgctgc ggcgactatc ttcccgccga gcagcaactg gcggcgcgct ttgaggtgaa
    17761 ccgccacacc ctgcgccgcg ccatcgacca actggtggaa aaaggctggg tacagcgccg
    17821 tcagggcgtc ggcgtgctgg tgctgatgcg cccgttcgat tacccgctca acgcccaggc
    17881 gcgttttagc cagaacctgc tggaccaggg cagccatccc accagcgaaa aactgctgtc
    17941 ggtattgcgc cccgcgtccg gtcacgttgc tgacgcgctg gggattaccg agggcgagaa
    18001 cgttatccat ttgcgcaccc tgcgccgggt caacggcgtc gcgctctgtt taatcgacca
    18061 ctactttgcg gacctcaccc tttggccgac gctgcaacgc ttcgacagcg gttcgctgca
    18121 cgattttctg cgcgagcaaa ccggaattgc gctgcgccgc agccagacgc ggatcagcgc
    18181 ccgccgcgcc caggccaaag agtgccagcg ccttgaaatc ccgaatatgt cgccgctgct
    18241 gtgcgtgcgc acccttaacc accgtgacgg tgaaagcagc ccggcggagt actccgtcag
    18301 cctgacgcgc gccgacatga ttgaattcac tatggagcac tgaatgcacg cagataccgc
    18361 gacccgccag cactggatgt ccgtactggc gcacagccaa ccggctgaac tggcagcccg
    18421 cctgaacgcg ctgaacatca ccgccgacta tgaagtgatc cgcaccgccg aaaccggcct
    18481 ggtacagatt caggcgcgaa tgggcggcac cggcgaacgc ttttttgccg gcgacgccac
    18541 gctgacccgc gccgccgtac gcctgactga cggcaccctc ggctacagct gggtgctggg
    18601 gcgtgataaa cagcacaccg aacgctgcgc gctgattgat gcgctgatgc agcaatctcg
    18661 ccactttcaa aacttatcag aaacccttat tgccccgctg gacgctgacc gtatggcacg
    18721 cattgccgca cgccaggccg aagtgaacgc cagccgggtc gacttcttta cgatggttcg
    18781 cggagacaac gcatgaccct ggaaaccgct tttatgcttc ccgtgcagga tgcccagcac
    18841 agttttcgtc gcctgttaaa ggccataagc gagccgggcg tgattgtcgc cctgcatcag
    18901 ctcaagcgcg gctggcagcc gctgaatatc gccaccacca gcgtgctgct gacgctggct
    18961 gataacgaca cgccggtgtg gcttgccgcg ccattgagta acgatatcgt caatcagagc
    19021 ctgcgttttc ataccaacgc gccgctggtc agccagccgg aacaggcgac cttcgcggtg
    19081 acggatgacg gaatttccag caaacagctc aacgcccttt ccaccggcac cgccgttgcg
    19141 ccggaagcgg gcgcgacgct gattttacag gtcgccagcc tgagcggcgg gcgcatgttg
    19201 cgtctcaccg gcgcgggtat tgccgaagaa cgaatgatcg ctccgcagct gccggagtgc
    19261 attctgcacg aactcaccga gcgcccgcac ccgttcccgc tcggcatcga cctgatcctg
    19321 acctgcggcg aacgcctgct ggctattccg cgaaccactc atgtggaggt gtgctgatgt
    19381 acgttgccgt gaaagggggc gaaaaggcga tcgacgccgc ccacgccctg caagagagcc
    19441 gacgccgggg cgataccgat ttgcccgaac tgagcgtcgc ccaaattgaa cagcagctta
    19501 atctcgcggt ggatcgcgtg atgaccgaag gcggcgttgc cgaccgcgaa ctggcggcgc
    19561 tggcgctgaa acaggccagc ggcgataacg ttgaagcaat tttcctgctg cgcgcctacc
    19621 gcaccacgct ggcgaaactg gcggtaagcg agccgctcga caccaccgag atgcgtctcg
    19681 aacggcgtat ttcggcggtt tataaagaca ttcccggcgg ccagctgctt ggcccaacct
    19741 acgactacac ccatcgcctg ctcgatttca ccctgctggc aaacggtgaa gcgccgacgc
    19801 tgaccaccgc cgacagcgaa caacagccgt cgccgcacgt tttcagcctg ctggcgcgtc
    19861 aggggctggc gaagtttgaa gaagatagcg gcgcacagcc ggatgacatc acccgcacgc
    19921 cgccggttta cccctgctca cgctcctccc gcctgcaaca attaatgcgc ggggacgaag
    19981 gctatttgct ggcgctggcc tactccaccc agcgtggtta cggacgcaat cacccgttcg
    20041 cgggcgagat ccgcagtggt tacatcgacg tgtcgattgt gccggaagag ctgggatttg
    20101 cggtaaacgt cggcgaactg ctgatgaccg agtgtgaaat ggtcaacggt tttatcgacc
    20161 cgccggatga gccgccgcac ttcacgcgcg gctacgggct ggtattcggc atgagcgagc
    20221 gcaaagcgat ggcaatggcg ctggttgacc gtgcgttgca agccccggaa tacggcgagc
    20281 acgcgacagg cccggcgcag gatgaagagt tcgtgctggt gcatgccgac aacgtcgaag
    20341 ccgcaggctt tgtctcgcac ctcaaactcc cccactacgt cgatttccaa gccgaactgg
    20401 agctactcaa acgtctgcaa caggagcaga accatggcta atctgagcgg ctacaacttt
    20461 gcctacctcg acgagcagac caaacgcatg atccgccgcg ccatcttaaa agcggtggct
    20521 atccccggtt atcaggtgcc gtttggcggg cgcgagatgc cgatgcccta cggctgggga
    20581 accggcggca ttcagctcac cgccagcgtg attggcgaaa gcgacgtgtt gaaggtgatt
    20641 gaccagggcg cagacgacac cactaacgcc gtgtcgattc gcaacttctt caagcgcgtg
    20701 accggggtga acaccactga acgtacggac gatgcgacgc ttatccagac gcgccatcgc
    20761 atccccgaaa cgccgctgac cgaagatcag atagttattt tccaggtgcc gatccccgaa
    20821 ccgctgcgct ttatcgagcc gcgcgaaacg gaaacccgca ctatgcacgc gctggaggag
    20881 tacggcgtga tgcaggtgaa actgtatgaa gatatcgccc gcttcggtca tatcgccacc
    20941 acctatgctt atccggtgaa ggtgaacggg cgctacgtga tggacccgtc gccgatcccg
    21001 aaattcgata acccgaaaat ggacatgatg cccgccctgc agctgttcgg cgcagggcgc
    21061 gagaagcgca tctatgcggt gccgccgttt acccgcgtgg aaagtctcga tttcgacgat
    21121 cacccgttca ccgttcagca gtgggatgag ccatgcgcca tctgcggatc gacccacagc
    21181 tatctcgacg aagtggtgct ggatgacgcc ggaaaccgca tgtttgtctg ctccgatacc
    21241 gattattgcc gccaacagag cgaggcaaaa aaccaatgaa tcaaccgtta ctttcggtca
    21301 ataacctgac ccacctttac gcgccgggca aaggctttag cgatgtctct tttgatttat
    21361 ggccggggga agtgctgggc atcgtcgggg aatccggctc cgggaagacc acgctgctga
    21421 aatcaatctc cgcgcgcctg acgccgcagc agggggagat tcgctacgag aatcgttcgt
    21481 tgtatgcgat gagcgaggcc gaccgccgtc gcctgctgcg caccgaatgg ggcgtggtgc
    21541 atcagcatcc actcgacggc ctgcgtcgcc aggtctcggc aggcggcaat atcggcgagc
    21601 ggctgatggc gaccggggcc cgtcattacg gcgacatccg cgccaccgcg cagaagtggc
    21661 tggaagaggt ggagattccc gccaaccgga tcgacgacct gccgaccacc ttttccggcg
    21721 gcatgcagca acgtttgcag attgcccgca acctggtgac gcatccgaag ctggtgttta
    21781 tggacgagcc aaccggtggg ctggatgtgt cggtgcaggc acgcctgctc gatctactgc
    21841 gcggcctggt ggtggagctg aacctggcgg tggtgattgt cacccatgat ttaggcgtcg
    21901 cccgcctgct agcggaccgt ttgctggtga tgaagcaggg gcaagtagtg gagagtgggt
    21961 taaccgaccg cgtgctcgac gacccgcacc atccgtatac ccagctgctg gtgtcatcgg
    22021 ttttgcagaa ttgagccggt gccggatgcg gcgtaaacgc cttatctggc ctacaaatgc
    22081 gctccccgta ggtcggataa gacgcatcag cgtcgcatcc gacacccgaa ccacgaggcg
    22141 aaaaatgatt aacgtacaaa atgtcagtaa aaccttcatc ctgcaccagc aaaacggcgt
    22201 gcgcctgccc gtcctcaacc gcgcctcgct caccgtcaac gcgggcgaat gcgtggtgct
    22261 ccacggccac tccggcagcg gcaaatcaac cctgctgcgc tcgctgtacg ccaactatct
    22321 gcccgatgaa gggcaaatcc agatcaaaca cggtgacgag tgggtagacc tggtcaccgc
    22381 gccagcgcgc aaagtggtgg agatccgcaa aaccaccgtc ggctgggtga gccagtttct
    22441 gcgcgtcatc ccgcgtattt cagcactgga agtggtgatg cagccgctgc tcgataccgg
    22501 cgtaccgcgt gaagcctgcg ccgctaaagc cgcgcgtctg ctcacccgcc tgaacgtgcc
    22561 ggaacgcctg tggcacctgg cgccatcaac cttctccggc ggcgaacagc agcgcgtcaa
    22621 catcgcccgc ggctttatcg tcgactaccc gattctgctg ctcgacgaac ctaccgcctc
    22681 gctggacgcc aaaaacagcg ccgcggtggt ggaactgatc cgcgaagcca aagctcgtgg
    22741 cgcagccatt gtcggcatct tccatgacga agccgtacgt aatgacgttg ccgaccgcct
    22801 gcacccaatg gaagcctctt catgattatc aataacgtca aattggtgct ggaaaacgaa
    22861 gtggtacacg gttcactgga ggtgcaggac ggcgaaatcc gcgcctttgc cgaaagccag
    22921 agccgcctgc cggaggcgat ggacggcgaa ggcggctggc tgctgccggg gctgattgag
    22981 ctgcataccg ataatctgga taaattcttc accccgcgcc cgaaagtcga ctggcccgcc
    23041 cattcggcga tgagcagcca cgacgcgctg atggtggcaa gcggcatcac caccgtactg
    23101 gacgccgtgg cgattggtga tgtgcgcgac ggcggcgatc ggttggagaa tctggagaag
    23161 atgatcaacg ccatcgaaga gacgcagaaa cgcggcgtca accgcgccga gcaccgtctg
    23221 ctcctgcgct gcgaactgcc gcatcacacc acgctgccgc tgtttgaaaa actggtgcag
    23281 cgcgagccgg tgacgctggt gtcgctgatg gaccactcgc cgggccagcg ccagttcgcc
    23341 aaccgcgaga agtatcgcga atattatcag ggcaaatact cgctcaccga tgcgcagatg
    23401 cagcagtacg aagaagagca actggcgctt gccgcacgct ggtcgcagcc gaatcgcgaa
    23461 tccatcgccg ccctgtgccg cgcgcgacaa attgcgctcg ctagccatga tgacgccacc
    23521 cacgcccacg ttgccgaatc ccacctgctt ggcagcgtga tcgccgaatt tcccaccacg
    23581 tttgaagcgg cggaagcctc gcgcaagcat ggcatgaacg tactgatggg cgcgccgaat
    23641 atcgtgcgca gcggttcgca ctccggcaac gtggcggcca gtgaactggc gcagcttggc
    23701 ctgctggata tcctctcttc cgactactac cccgccagcc tgctggatgc ggcatttcgc
    23761 gtcgccgatg accagagcaa ccgctttacg ctgccgcagg cggtgaagtt agtgacccaa
    23821 aatccggcgc aggcgcttaa tcttcaggat cgcggggtga ttggcgaggg caaacgcgcg
    23881 gacctggtgc tggcacatcg caaggacaat catattcata tcgaccacgt ctggcgtcag
    23941 ggtaaaaggg tgttctgatg atgggaaaac tgatttggtt aatggggccg tccggctccg
    24001 ggaaagacag cctgctggcg gaaatccgcc tgcgggaaca aactcagtta ctggtggcgc
    24061 atcgctacat tacgcgcgac gccagcgccg gaagtgaaaa ccatatcgcc ctgagcgagc
    24121 aggagttttt tacccgcgcg gggcaaaatc tgttggcctt aagctggcac gctaacagtc
    24181 tgtattatgg cgtcggcgtt gagattgatc tctggctgca cgccggattc gacgtgctgg
    24241 tcaacggctc acgcgcccat ctgccgcagg cgctggcgcg ctatcaatcg gcgctgctgc
    24301 ccatttgctt acaggtttcg ccggagatcc tgcgccagcg cctcgaaaac cgtggtcgtg
    24361 aaaatgccag tgaaattaac gcccgactag cacgcgctgc ccgctatact cctcaggatt
    24421 gtcttacgct caataatgac ggcagcctgc gccagtcggt cgacaagctg ctgacgctga
    24481 ttcatcagaa ggagaaacac catgcctgct tgtgagcttc gcccggctac gcagtacgac
    24541 accgacgcag tttacgcgct gatttgtgag ctaaaacagg cggagtttga ccaccatgcg
    24601 tttcgcgtgg gctttaacgc caacctgcgc gacccaaaca tgcgctacca tctggcgctg
    24661 ctggatggcg aagttgtcag catgatcggc ctgcatttgc agtttcatct gcatcatgtc
    24721 aactggatcg gcgaaattca ggaactggtg gtgatgccgc aggcgcgcgg tctgaacgtc
    24781 ggcagtaaat tactggcatg ggcagaagaa gaagcccgcc aggccggggc cgaaatgacc
    24841 gcgctttcga ccaacgtgaa acgccacgac gcgcaccgtt tctatctgcg agaaggctac
    24901 gagcagagtc acttccgctt caccaaggtg ctgtaacatg agcctgaccc tcacgctcac
    24961 cggcactggc ggcgcacagg gcgttccggc atggggctgc gagtgcgcgg cctgcgaccg
    25021 agcgcagcgc tcaccgcagt atcgccgcca gccatgcagc ggcgtagtga agtttaacga
    25081 cgcaatcacc ctgatcgacg ccgggctgca cgatctcgcc gatcgctggt cgcccggatc
    25141 gttccagcag tttttgctga cgcattatca tatggatcac gcccaggggc tgtttccgct
    25201 gcgctggggc gtgggcgatc cgatcccgat ctacggcccg ccggatgacc agggttgcga
    25261 cgatctgttt aaacatccgg gcctgcttga tttcagccac acagtggaac cgtttgtggt
    25321 gtttgatttg taggggttgc aggtcacgcc gctgccgctt aaccactcaa aactgacctt
    25381 cggttatctg ctggaaacgg cacatagccg ggtggcgtgg ctatctgaca ccgctggcct
    25441 gccggaaaaa acgctgaaat ttttacgcaa taatcagcca caggtaatgg tgattgattg
    25501 cagccacccg ccgcgcgcgg atgcgccgcg taatcactgt gatttaaata ccgtgcttgc
    25561 gctgaatcag gttatccgct cgccacgggt gattctgacc catatcagcc accagtttga
    25621 tgcgtggctg atagaaaacg cactgccgtc ggggtttgag gcggggtttg atgggatgga
    25681 gattggggtg gcgtgatgag agggaatgtg cgcgctggtc ccctcaccct aaccctctcc
    25741 ccagaggggc gaggggagcg attgtgctcg atattgaata ttgcgctggt tttctccctc
    25801 tccccattgg ggtgaggggc gatgcctgct ccatacccaa cctcatcgcc catactcatc
    25861 ttccattctc cgctcttcat cttctagctg ccgacgctcc tgatcaagct ggcgctggcg
    25921 atcgtccagc tgcctgcggc gatcttcaaa ctggcagcgg cggtcgtcat attgtctgcg
    25981 ccgatcgtcg ctcacttcac gctgccagcc gtcgtcgcgc gaatcttcat agtctcgccc
    26041 acggtcagag ttataagcgt cattaatcgc ctgctgaata ttaccaatag tatcgtcgat
    26101 aatatcggcc tgggccggaa cggtggacag cgtgagcagg gtgaaaagaa aaagcgtgga
    26161 gtaaggtttc ataagccagt ctctgcgatg gactggctta acgttagttg agtgtgaatg
    26221 accggagtag cggagtaatc tcaaattcgg taaggcgcag actcttccat tagctggtta
    26281 gctcgctgtt tattttccgc caccaataca atgccatgac atttttgatc cggtgaaata
    26341 ctccagatac gctcttccgg aatgcagccg ccatcgtaga tcactttcgt gccatcgtag
    26401 ggccaaaatg ccccttgttc agtgtctaat acaaacacgg aaatctgatc ggactcgcgc
    26461 gcgatcttct gccagagcat cttcccgcca tcatattgat caaaatcgct gattagcgtg
    26521 ataccgcatt gcaggattag cgcttcatac agccgagttg ccagaccctt atcgcgctca
    26581 cctggagaga cacaaatacc ttccacctgt accagatccg agtcttccca ccagcgttca
    26641 acggcaatga tatcgctgcc gaactctttt ttacgctgca gatcgagagc ggcaagtacc
    26701 cgatgacgcg gcacgcctgc aaaaacttca atgtccgctt caatgatggc cactcttggc
    26761 gtctggttgt cataaattac ctgtaacaga cgtccagaaa gctcgatctc ttccaccacg
    26821 cgaaaagaac gagcaaccgt catagggcta taggtggttg gcatcacgat catatgtaaa
    26881 tcacttcaat tcagaacaga attgctcaca atagcagcac gtttgcttcg agcctcttga
    26941 taaattctga aaatcatccc tcaatttgcc ccagcacctg cgccagctgc gcgcgggtaa
    27001 agggtttgcg cagcagcgcc acgtccggca gcgcggggtt atggctgggg cgtaaatcct
    27061 gaccgctgat gagtagcagc gtcaggtgcg ggtaaagttt gcgggccgca ttgaccactt
    27121 ctgcgccgct catgccgcca gcaggtccag cgcctgttcg ccgctgctcg cttccagtgt
    27181 caaatagccc agcaggtgca actgctcgca gatggtctgg cgcaccgcgg cttcatcttc
    27241 cagcactaac accagtttat cgccattaac agctgtctgt tctaccgccg ctggcgatag
    27301 gtttttcgcc gccgtcagcg cgcggggaag ctgtaatctg accgttgtcc cctgccccgg
    27361 cgcgctttca atttccacgc gcccgccgga ctggcgcacg aaaccataga ccatcgacaa
    27421 gcccagcccg ctgccgctcc ccgtctgttt ggtggtaaag aacggctcga acacctggct
    27481 tttcaccgcc tgcgacatac cgctgccatg atcgatgacc tccagtgcca ccatgtcctg
    27541 acgctgccca ctgctgcggg taacacgctg attccaggta cgaattttga taattcccgt
    27601 ctgcccttcc atcgcgtcgc gggcgttcat caccaggttg atgatggcgt tttccagttg
    27661 gctgacgtca atccacgcgg accacgccgg agactgcgct tcaatctcaa tcgtcagcgt
    27721 cgccggtaag gagtggcgca ttaattcgct taactcttcc agcagtgttt ttagttcaac
    27781 ggcttgcgga tgtaaggact gcttgcggga aaatgccaac aatcgctggg tcagtagcgc
    27841 gccacgttcg gcggcttgct gcgcccgctg tattcgcggc gcgtcgggtg agttcgtgtt
    27901 caccagttcc aggctaccaa taattaccgc cagcagatta ttaaaatcat gggccagccc
    27961 gcccgtcagt tgcctgaccg cttttaactt ctggctgtgc aatagcgcct cttccagccc
    28021 ctgccgctcg gtttgggcaa tctccatctg cgtggttttc tctttcaaca gccgtgacgt
    28081 tcgttcaagt gatgcggtat tgcgggcaaa aacgttaaag gcgcgcgcca gttcacccaa
    28141 ctcatcgcgc cgctgtaacg ccggaaaagt gacgtccgct tcgccgtgcg ccaggcggct
    28201 catcgcgcgg gaaatcgccg tcaggttaga gccgagattg cgatagatat accaacctgc
    28261 aaacgcggtg atcaccaccg ctaacagggc gaaaatcatg atcagcctct cgccggagcg
    28321 caaatcctgg taactttgct ccacccgctg accagagagc gtcgccacct gttcggcgta
    28381 acggttgatg tcgccattaa ggatcgccac cagcgctttg atgtgaaaca tataccagct
    28441 gattgccaga tcgctttgct ccagagccgc cgaaagtggc gcgagtttac gcagttcacg
    28501 attaaaatca gcgagtagcg tattaaccaa tggcgtatcg ctgtgagttg gcaaggcaga
    28561 catcactccg accagttgat gaataatcgc ctgcggcgcg ggggtggcaa tggcggcaac
    28621 aatcagtcgg ttcatctcgt taagcaaaat attatcttgc gcgtgagtga ctttttgcag
    28681 atgccgcaga taacttaagt tttgatacag cgaacttaac agcgcgttac gctccagatg
    28741 ccgcctttgc ccgcgttcca gtattccgcc gacgctggtt tgcagttcgt tgctacgcgt
    28801 gatgatgcgc gtcaccagcc cggcttcctg ctgcgctaaa ggcgcgttcg ccagttgtgc
    28861 aagcgagctt tttaatgccc gctgtgtctc ctgcaaccgc tcggcttcgc ctttatattc
    28921 cagtgctccc accacctgcg acaatcgcac tgccgccgtt gccacgtttg ccgtgtcgcg
    28981 cgccagattc aggctgccgg tcatatcatc tacagtttgc tgctgcacca gctcttgcat
    29041 ctggctggca tgacgaaagc ccattaccgc cacgccgctg accatcaacg tcaccgccac
    29101 caccagcaaa ttcaaaagca gcaatcgccc gcgcgcgctg gcaaaaaagt gacgtccggg
    29161 gtgcgccata tcacgctccg tgaagaaaaa tcgcaatatg acaaatatga acggcttctg
    29221 acattttgca tttactttcc tgtgatggag tgctgatatc gacaacctcg caggagtcca
    29281 ccatgaacag cacgccggtt cttgagatgc gcaatattgc caa
//
LOCUS       DADMEQ010000043        29082 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.043, whole genome shotgun sequence.
ACCESSION   DADMEQ010000043 DADMEQ010000000
VERSION     DADMEQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.043"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            71..1348
                     /gene="waaA"
                     /locus_tag="JNZ05_001904"
     CDS             71..1348
                     /gene="waaA"
                     /locus_tag="JNZ05_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HAY8565152.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1356..1835
                     /gene="coaD"
                     /locus_tag="JNZ05_001905"
     CDS             1356..1835
                     /gene="coaD"
                     /locus_tag="JNZ05_001905"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAY8565153.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1874..2683)
                     /gene="mutM"
                     /locus_tag="JNZ05_001906"
     CDS             complement(1874..2683)
                     /gene="mutM"
                     /locus_tag="JNZ05_001906"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HAY8565154.1"
                     /translation="MPELPEVETSRRGIEPNLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2781..2948)
                     /gene="rpmG"
                     /locus_tag="JNZ05_001907"
     CDS             complement(2781..2948)
                     /gene="rpmG"
                     /locus_tag="JNZ05_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAY8565155.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2969..3205)
                     /gene="rpmB"
                     /locus_tag="JNZ05_001908"
     CDS             complement(2969..3205)
                     /gene="rpmB"
                     /locus_tag="JNZ05_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAY8565156.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRTRGEKY"
     gene            complement(3422..4090)
                     /locus_tag="JNZ05_001909"
                     /pseudo
     CDS             complement(3422..4090)
                     /locus_tag="JNZ05_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            4262..5482
                     /gene="coaBC"
                     /locus_tag="JNZ05_001910"
     CDS             4262..5482
                     /gene="coaBC"
                     /locus_tag="JNZ05_001910"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAY8565157.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5460..5918
                     /gene="dut"
                     /locus_tag="JNZ05_001911"
     CDS             5460..5918
                     /gene="dut"
                     /locus_tag="JNZ05_001911"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAY8565158.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYTTSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6025..6621
                     /gene="slmA"
                     /locus_tag="JNZ05_001912"
     CDS             6025..6621
                     /gene="slmA"
                     /locus_tag="JNZ05_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HAY8565159.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6658..7299)
                     /gene="pyrE"
                     /locus_tag="JNZ05_001913"
     CDS             complement(6658..7299)
                     /gene="pyrE"
                     /locus_tag="JNZ05_001913"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAY8565160.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7365..8081)
                     /gene="rph"
                     /locus_tag="JNZ05_001914"
     CDS             complement(7365..8081)
                     /gene="rph"
                     /locus_tag="JNZ05_001914"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAY8565161.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8208..9071
                     /locus_tag="JNZ05_001915"
     CDS             8208..9071
                     /locus_tag="JNZ05_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAY8565162.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9292..10116
                     /gene="dinD"
                     /locus_tag="JNZ05_001916"
     CDS             9292..10116
                     /gene="dinD"
                     /locus_tag="JNZ05_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HAY8565163.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            10407..11024
                     /locus_tag="JNZ05_001917"
     CDS             10407..11024
                     /locus_tag="JNZ05_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HAY8565164.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11021..12703)
                     /gene="ligB"
                     /locus_tag="JNZ05_001918"
     CDS             complement(11021..12703)
                     /gene="ligB"
                     /locus_tag="JNZ05_001918"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HAY8565165.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPSRAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12961..13584
                     /gene="gmk"
                     /locus_tag="JNZ05_001919"
     CDS             12961..13584
                     /gene="gmk"
                     /locus_tag="JNZ05_001919"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAY8565166.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13639..13914
                     /gene="rpoZ"
                     /locus_tag="JNZ05_001920"
     CDS             13639..13914
                     /gene="rpoZ"
                     /locus_tag="JNZ05_001920"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAY8565167.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            13933..16041
                     /gene="spoT"
                     /locus_tag="JNZ05_001921"
     CDS             13933..16041
                     /gene="spoT"
                     /locus_tag="JNZ05_001921"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HAY8565168.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16048..16737
                     /gene="trmH"
                     /locus_tag="JNZ05_001922"
     CDS             16048..16737
                     /gene="trmH"
                     /locus_tag="JNZ05_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HAY8565169.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16743..18824
                     /gene="recG"
                     /locus_tag="JNZ05_001923"
     CDS             16743..18824
                     /gene="recG"
                     /locus_tag="JNZ05_001923"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAY8565170.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18858..20063)
                     /gene="gltS"
                     /locus_tag="JNZ05_001924"
     CDS             complement(18858..20063)
                     /gene="gltS"
                     /locus_tag="JNZ05_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HAY8565171.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20343..21734
                     /gene="xanP"
                     /locus_tag="JNZ05_001925"
     CDS             20343..21734
                     /gene="xanP"
                     /locus_tag="JNZ05_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HAY8565172.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSDFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21855..23564
                     /locus_tag="JNZ05_001926"
     CDS             21855..23564
                     /locus_tag="JNZ05_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY8565173.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     QLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23617..25935)
                     /gene="yicI"
                     /locus_tag="JNZ05_001927"
     CDS             complement(23617..25935)
                     /gene="yicI"
                     /locus_tag="JNZ05_001927"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HAY8565174.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEYFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25945..27328)
                     /locus_tag="JNZ05_001928"
                     /pseudo
     CDS             complement(25945..27328)
                     /locus_tag="JNZ05_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27621..27715
                     /locus_tag="JNZ05_001929"
     tRNA            27621..27715
                     /locus_tag="JNZ05_001929"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27655..27657,aa:Sec,seq:tca)
     gene            27987..29082
                     /locus_tag="JNZ05_001930"
                     /pseudo
     CDS             27987..29082
                     /locus_tag="JNZ05_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         7183 a         7791 c         7309 g         6799 t
ORIGIN      
        1 ggaaaagtaa tggtaaagcc acagctaaat acatagaatc cccagcacat ccataagtca
       61 gctatttact atgctcgaat tgctttacac cgcccttctc taccttattc agccgctgat
      121 ctggatacgg ctctgggtgc gcggacgtaa ggctccggcc tatcgaaaac gctggggtga
      181 acgttacggt ttttaccgcc atccgctaaa accaggcggc attatgctgc actccgtctc
      241 cgtcggtgaa actctggcgg cgatcccgtt ggtgcgcgcg ctgcgtcatc gttatcctga
      301 tttaccgatt accgttacaa ccatgacgcc aaccggttcg gagcgcgtac aatcggcttt
      361 cgggaaggat gttcagcacg tttatctgcc gtatgacctg cccgatgcgc tcaaccgttt
      421 cctgaataaa gtcgacccca aactggtgtt gattatggaa accgaactat ggcctaacct
      481 gattgcggcg ctacataaac gtaaaattcc gctggtgatc gctaacgcgc gactctctgc
      541 ccgctcggcc gcaggttatg ccaaactggg taaattcgtc cgccgcttgc tgcgtcgtat
      601 tacgctgatt gctgcgcaaa atgaagaaga tggtgcacgt tttgtggcgc tgggcgcaaa
      661 aaataaccag gtaaccgtta ccggtagcct gaaattcgat atttctgtaa ctccgcagtt
      721 ggctgctaaa gccgtgacgc tacgccgcca atgggcacca caccgcccgg tatggattgc
      781 caccagcact cacgaaggtg aagagagcgt ggtcattgcc gcacatcagg cattgttaca
      841 gcaattcccg aatttattgc tcatcctggt accccgtcat ccagaacgct tcccggatgc
      901 gattaacctt gtccgccagg ctggactaag ctatatcaca cgctcttcag gggaagtccc
      961 ctccaccagc acgcaggttg tggttggcga tacgatgggc gagttgatgt tactgtacgg
     1021 cattgccgat ctcgcctttg ttggcggttc actggttgaa cgtggtgggc ataatccgct
     1081 ggaagctgcc gcacacgcta ttccggtatt gatggggccg catactttta actttaaaga
     1141 catttgcgcg cggctggagc aggcaagcgg gcttattacc gttaccgatg ccactacgct
     1201 tgcaaaagag gtttcctctt tactcaccga cgccgtttac cgtagtttct atggccgtca
     1261 tgccgttgaa gtactgtatc aaaaccaggg cgcgctacag cgtctgcttc aactgctgga
     1321 accttacctg ccaccgaaaa cgcattgagg ttgttatgca aaaacgggcg atttatccgg
     1381 gtactttcga tcccattacc aatggtcata tcgatatcgt gacgcgcgcc acgcagatgt
     1441 tcgatcacgt tattctggcg attgccgcca gccccagtaa aaaaccgatg tttaccctgg
     1501 aagagcgtgt ggcactggca cagcaggcaa ccgcgcatct ggggaacgtg gaagtggtcg
     1561 ggtttagtga tttgatggcg aacttcgccc gtaatcaaca cgctacggtg ctgattcgtg
     1621 gcctgcgtgc ggtggcggat tttgaatatg aaatgcagct ggcgcatatg aatcgccact
     1681 taatgccgga actggaaagt gtgtttctga tgccgtcgaa agagtggtcg tttatctctt
     1741 catcgttggt gaaagaggtg gcgcgccatc agggcgatgt cacccatttc ctgccggaga
     1801 atgtccatca ggcgctgatg gcaaaattag cgtagcgttt atgccggatg gtatgccatc
     1861 cggctcgcat gaattacttc tggcactgcc gacaataaaa cgttgcccgc tgcgcatgtt
     1921 tagtcgccac aatcggcgta ccgcacaccc gacacggctc accttttcgc ccgtaaacct
     1981 gcaattcctg cgcgaaatag cccggtttac catcactttg cagaaaatct ttcagcgttg
     2041 taccaccctg ctcaatcgaa cgcagcaaca ccgctttaat cacccgagct aacaattcac
     2101 actctgccag cgacagtgat gacgccagcc gatccggatg gatccccgcc gcaaacagtg
     2161 attcgctggc atagatattc cctaccccta ccaccagctt gttatccatc agccacggtt
     2221 taatcgccgt ttttttcttc gcgcacttct gatgcagata ctcaccattg aaatcgtcgc
     2281 taagcggctc cggtccaaga tgggccagca cattatgccc ttccagctct ttggtccaca
     2341 gccaggcacc aaagcggcgc ggatcggtgt agcgcagcac tttaccgttg ctcatcacca
     2401 aatccacatg gtcatgcttt tcagggggaa gttcttctgg aaggatgcgc aggctgccag
     2461 acatccccaa atgaatgata atccagccct caggcagctc cagcagcaga tatttagccc
     2521 gccgctgcac gctaagcact ggttgatcgc ttaatcggta gatctcttct gaaaccggcc
     2581 agcgcaagcg tccgttgcgc accaccgcat gaagaatggt tgcaccaacg agattcggtt
     2641 ctatgccgcg acggctggtt tcaacttcgg gtaattcagg catagcatct ccaggaatga
     2701 acagatgcaa tcaatatggg ggcaagcaga taacaaaaaa ccccgccgga gcgaggtttt
     2761 tcgttacatc aaagcgagaa ttatttgatt ttcgcttctt tgtagatcac gtgctgacga
     2821 acaactggat cgaatttttt cagttccagt ttttccggct tagtacgttt gttcttcgta
     2881 gtggtataga agtgaccagt accagcagaa gaaaccagct tgattttctc acgaatacct
     2941 ttagccatga tttatttcct ctaagtactt agtacttttc gccacgggta cgcagttcag
     3001 ccagaactgt atcgatgcct tttttatcga ttacacgcat acctttagca gatacgcgca
     3061 gggtgacaaa acgcttctcg ctctcaaccc agaaacggtg agagtgcagg ttcggcagga
     3121 aacggcgttt agtcgcgttc agtgcgtggg aacggttgtt accggtcacc ggacgcttgc
     3181 cagtaacttg gcagactcgg gacatgtcta ttctccaaaa atcaaattag ctcgagcttc
     3241 gtatggggta ttggcgcctc gtcaggcttt acagcccggt catcgcagtt ctatgtgaac
     3301 tctcgattgc caggcccaaa tgccaaaccc gagattctca aaggtggcgt agtatacgct
     3361 gactcagcga tgtgctcaag tcccgaacag acaaagatcc cgaaggatcg cgcatagcgg
     3421 gttaaatcca gccgcgttcg gcaaaagaaa catactctcc acgcccaatc acgatatggt
     3481 cgagcacgcg taaatccatg aactgacaac tctttattat ccgttcagta atgagtttat
     3541 ccgctttact gggttcagca caacccgaag ggtgattatg tgcaaggatc agcgccgagg
     3601 cgtttatttt tatcgcttag cggataattt cccgaggatg gacttcaaca tggtttagcg
     3661 tgccggaaaa aagacggcta tgcgttataa cccggtgttg tgagtcgaga aagatcacca
     3721 taaagatctc ccgctcctca cccgtgagct ggctttgtaa aaattcccgc gtcatctccg
     3781 ggctgagtaa agggctttct tcacgcatcc gcacgttgta gtaacgtcgc gccagttcag
     3841 caatcccctt taactgggca aatttcgcta cgccaattcc atgaacacca ctaaattgtt
     3901 catattcaga ggttaacaag ccataaagag agccgaaatt ctccagcatc tcttttgcca
     3961 gggttaatac atctttaccg cgcgttccgg tacgcagaaa tagcgccagc agctcgacat
     4021 ccgttaaggc gctaatacca aacttcagca ttttttcgcg cggcatcaac agctgtgcat
     4081 tgtttttcac cttcacctcc tttgtggtgc tcgcatcctg tcatagccgt tccgttaaat
     4141 cgacggccag ttttcaatcc tggaaagagc ctcgcaaagt gaatctcagg tgaaggcacg
     4201 acaacaaaag tattgtgata aaatcgccaa cttctggtgt cacccaacag gaaaaatcat
     4261 catgagcctg gccggtaaaa aaatcgttct cggcgttagc ggcggtattg ctgcctataa
     4321 aacccctgaa ctggtgcgtc gtttgcgcga tcgcggggcc gacgtccgcg tagccatgac
     4381 cgaagcggca aaagccttta tcaccccact tagcttgcag gcggtttctg gttatcccgt
     4441 ttccgacagt ctgcttgacc cggcagccga agccgctatg ggccatattg agctgggtaa
     4501 atgggctgat ttagtgattc tcgcccctgc cacggcagat ttgattgccc gtgttgctgc
     4561 cggaatggcg aatgacctgg tatcgacgat ttgtctggct acacctgcgc ctgtagccgt
     4621 gctccccgcc atgaaccagc agatgtaccg tgccgctgcc acgcagcata atttagaggt
     4681 gcttgcttcc cgtggtttgc tcatctgggg gccagacagt ggcagtcagg cttgtggtga
     4741 tatcggtcct ggacgaatgc tcgatccgtt aaccattgtg gatatggcgg tggcgcattt
     4801 ttcgcccgtc aacgacctga aacatctgaa cattatgatt accgccggtc cgacgcgtga
     4861 accgctcgat ccggtgcgtt atatctctaa tcacagctcc ggcaagatgg gttttgctat
     4921 cgccgccgct gctgcccgtc gtggcgcgaa cgtcacgctg gtatcaggtc cagtttcact
     4981 accgacgcca ccgtttgtta aacgtgttga tgtgatgacc gcgctggaaa tggaagccgc
     5041 cgtgaatgct tctgtacagc agcaaaatat ttttatcggt tgcgccgccg tggcggatta
     5101 tcgcgcagct accgtggccc cagaaaaaat caaaaagcag gccacgcagg gtgatgaatt
     5161 aacaataaaa atggttaaaa accccgatat cgtcgcaggc gttgccgcac taaaagacca
     5221 tcgaccctac gtcgttggat ttgccgccga aacaaataat gtggaagaat acgcccggca
     5281 aaaacgtatc cgtaaaaacc ttgatctgat ctgcgcgaac gatgtttccc agccaactca
     5341 aggatttaac agcgacaaca acgcattaca ccttttctgg caggacggag ataaagtctt
     5401 accgcttgag cgcaaagagc tccttggcca attattactc gacgagatcg tgacccgtta
     5461 tgatgaaaaa aatcgacgtt aagattctgg acccgcgcgt tgggaaggaa tttccgctcc
     5521 cgacttatac cacctctggc tctgccggac ttgacctgcg tgcctgtctc gacgacgccg
     5581 tagaactggc tccgggtgac actacgctgg ttccgaccgg gctggcgatt catattgccg
     5641 atccttcact ggcggcaatg atgctgccgc gctccggatt gggacataag cacggtatcg
     5701 tgcttggtaa cctggtagga ttgatcgatt ccgactatca gggccagttg atgatttccg
     5761 tgtggaaccg tggtcaggac agcttcacca ttcaacctgg cgaacgcatc gcccagatga
     5821 tttttgttcc ggtagtacag gctgaattta atctggtgga agatttcgac gccaccgacc
     5881 gcggtgaagg cggctttggt cactctggtc gtcagtaaca catacgcatc cgaataacgt
     5941 cataacatag ccgcaaacat ttcgtttgcg gtcatagcgt gggtgccgcc tggcaagtgc
     6001 ttattttcag gggtattttg taacatggca gaaaaacaaa ctgcgaaaag gaaccgtcgc
     6061 gaggaaatac ttcagtctct ggcgctgatg ctggaatcca gcgatggaag ccaacgtatc
     6121 acgacggcaa aactggccgc ctctgtcggc gtttccgaag cggcactgta tcgccacttc
     6181 cccagtaaga cccgcatgtt cgatagcctg attgagttta tcgaagatag cctgattact
     6241 cgcatcaacc tgattctgaa agatgagaaa gacaccacag cgcgcctgcg tctgattgtg
     6301 ttgctgcttc tcggttttgg tgagcgtaat cctggcctga cccgcatcct cactggtcat
     6361 gcgctaatgt ttgaacagga tcgcctgcaa gggcgcatca accagctgtt cgagcgtatt
     6421 gaagcgcagc tgcgccaggt attgcgtgaa aagagaatgc gtgagggtga aggttacacc
     6481 accgatgaaa ccctgctggc aagccagatc ctggccttct gtgaaggtat gctgtcacgt
     6541 tttgtccgca gcgaatttaa ataccgcccg acggatgatt ttgacgcccg ctggccgcta
     6601 attgccgcgc agttgcagta atatgacgcc ggatgacttt tcatccggcg agtttcttta
     6661 aacgccaaac tcttcgcgat aggccttaac cgccgccaga tgttccgcca tttccggctt
     6721 ctcttccaga taggcaatca gatctttcag ggtgatgata gagatcactt tgcagttgta
     6781 atcacgctca acttcctgaa tcgccgaaat ctcgccgcgc ccgcgttcct gacgatcgag
     6841 cgaaatcaac acgccagcaa gcgtcgcgcc attggcctga ataatctcca tcgactcgcg
     6901 aatcgccgtt ccggcggtga tcacatcatc taccagcatt acgcgtcctt gtaacgcgct
     6961 accaaccaga ttgccgcctt caccgtggtc ttttgcttct ttgcggttaa agcagtacgg
     7021 caggtccagg tcgtgatgct ccgccagtgc cacagcggtt gtggtggcaa tcgggatccc
     7081 tttgtaagca gggccaaaca gcagatcgaa ctcaatgccg gaatccacca acgcttcagc
     7141 gtaaaaacgg cctaacagtg ccagatcgcg cccggtatta aacagcccgg cgttgaagaa
     7201 ataggggctt ttgcgcccgg atttcagcgt aaactcgcca aactttaaca cctgcttgct
     7261 aagcgcaaat tcaataaact ggcgctgata tggtttcatg ccttcgctcc tcatcttact
     7321 tttctacaga caaaaaaaag gcgactcatc agtcgcctta aaaatcagtt tgccagcgcc
     7381 gccttctgcg tcgctacaat ggattcgatt ccccctcggg ccagcgccaa caaggtgagt
     7441 agctcttcat gggtgaacgg ctcgccttct gccgtcccct gcacttcaat gatgcgcccg
     7501 tcttcggtca tcactacgtt catgtcggtc tctgcggctg agtcttcaac gtattccaga
     7561 tcgcaaattg cttcaccgtt tacaataccg acagaaactg cggctaccat ccctttcatc
     7621 gggttggttt tcagcttgcc gttttccacc agcttctgta gcgcatctgc cagcgccacg
     7681 caggcacccg taatcgacgc ggtacgagtg ccaccatcag cctgaagcac gtcgcagtcc
     7741 agcgtaatgg tgaactcacc cagcgctttc aaatctactg ccgcgcgaag agcacgggcg
     7801 atcagacgct ggatttccat tgtgcgtcca ccctgcttac ctttcgccgc ttcacgagcg
     7861 ttacgggtgt gggtagaacg tggcagcatg ccgtactctg cggtgatcca gccctggccc
     7921 tgacctttca ggaagcgcgg cacgccttct tcaatagagg cggtacacaa tactttggta
     7981 tcgccaaatt cgaccagcac cgagccttct gcatgttttg tatagttacg agtcagggta
     8041 acgggacgca cctgattatt gctacggcct gctggacgca tattgaaatc tccggcttga
     8101 aacaaatgtg gctgcgcatt atacggactt ccggcggtta ttcctatcct gacaaggcat
     8161 cgatggctat aatccttcca cctctccttt tataaacagg aacgtctatg atccgcagta
     8221 tgaccgccta cgcccggcgt gaaatcaagg gtgaatgggg gagcgcaact tgggaaatgc
     8281 gctcggtaaa ccagcgttat ctggaaactt actttcgtct gccggagcag ttccgtagcc
     8341 ttgaacctgt cgttcgcgag cgtattcgtt ctcgcctgac gcgcggtaaa gtggaatgta
     8401 ccctgcgcta tgagccagat gttagcgcac aaggtgagct gatcctcaat gaaaaactgg
     8461 ctaaacagct ggtaactgcc gcgaactggg taaaaatgca gagtgacgaa ggggaaatca
     8521 acccggttga tattctacgc tggccgggcg tgatggcagc ccaggagcag gatctcgacg
     8581 cgattgccgc tgaaattctc gcggcgttgg atggtacgct ggacgacttt atcgtcgcgc
     8641 gcgaaaccga aggtcaggca ctgaaagcat tgatcgagca gcgtctggaa ggcgtcaccg
     8701 ccgaagtggt caaagtccgc gcccatatgc cggaaattct gcaatggcag cgtgagcgtc
     8761 tggtcgcgaa gctggaagat gctcaggttc agttggaaaa caaccgtctg gagcaggagt
     8821 tggttctgct ggcacaacga attgacgttg ccgaagagct ggaccgcctc gaagcgcatg
     8881 ttaaagagac gtacaacatt ctgaagaaaa aagaagcggt tggtcgtcgt ctggacttta
     8941 tgatgcagga gttcaaccgt gagtcgaaca ctcttgcgtc gaaatctatc aatgccgaag
     9001 tgacaaactc cgccatcgag ctgaaagtgt tgattgagca gatgcgcgag cagattcaga
     9061 atattgagta actcgtaaca ccaattctga cttttcagcc ttcacaaatg ccgcccactc
     9121 aaacagagcg gcatttttct tccccgcaac ttcaattctg ttttgcgtgc ctgctccaga
     9181 ttttgcgatg tttttttgcc cagcacactg agaacgtgag atactcacaa ctgtatataa
     9241 atacagttac agatttactt tctttgcaat cgatatcaca tggagtgggc aatgaacgaa
     9301 catcatcaac cttttgaaga gataaaactg attaatgcaa acggagcaga acaatggtca
     9361 gcaagacaac ttgggaaact actgggttat tcagagtatc gtcactttat acctgtatta
     9421 acgcgcgcca aagaggcctg tgagaacagt ggtcacacaa ttgatgacca tttcgaggag
     9481 atcctcgata tggtcaaaat tggctcaaat gccaaaagag cattaaaaga catcgtactc
     9541 tcccgctatg cctgttacct ggtagtacaa aacggcgacc ccgcgaaacc ggtcattgcg
     9601 gcagggcaga cttattttac tatccagacc cgacggcagg agcttgctga cgatgaagca
     9661 tttaagcaac ttcgtgaaga cgaaaagcgt ctgtttctga gaaatgagtt gaaagaacat
     9721 aacaaacaat tggttgaggc agcacagcaa gctggggtag cgacagctac tgatttcgcc
     9781 atatttcaga atcatggtta ccaggggctg tatggtggat tagatcagaa agccatccac
     9841 caactgaagg ggctgaaaaa gagtcagaag atcctggatc atatgggttc aacagaactg
     9901 gcggctaatc tctttcgagc tacccaaaca gaagaaaaac tcaagcggga tagcgtgaat
     9961 tcaaaacagc aagctaatac cacacacttt gacgtgggta gcaaggtgag gcaaaccatt
    10021 caggaacttg gcggaaccat gcctgaggag ttgccgaccc cgcaggtcag catcaagcag
    10081 ttggaaaaca gtgtaaaaat tacagagaag aaatagcaga aacgaaaaac tctttttgtt
    10141 gattgagaca cccgatgcgt aagccaaggt ccaggtgcag tgaacacaat ggctaaacat
    10201 tgcacctttc ttttcccccc tcagttttaa cctatttttt cttatgcatt ttctcagaca
    10261 agaagtcaga agaatgcatc tacgctacag aaaatagcga tttcacataa ctaaaagtta
    10321 tcaatttcct ctcaccttaa aaaatatcaa tcgtgacaat gcgcacaaat cgctaccctg
    10381 ccagacagat ttttagggag agaaccatgc tgttacacat tttgtatttg gttggcatta
    10441 ctgccgaagc catgacgggg gcgctggcgg ccgggcgacg gcgcatggat acatttggcg
    10501 taattattat tgcaaccgcc accgcaattg gcggagggtc agtgcgcgat attctgctgg
    10561 gccactatcc gctcggctgg gtcaaacacc cggaatatgt gattatcgtc gctaccgccg
    10621 cagtactcac cactatcgtt gctcctgtaa tgccttacct gcgcaaagtg tttctggtgc
    10681 tcgatgcgct cggactggtg gtcttttcta tcatcggcgc acaggttgca ctggatatgg
    10741 ggcacggccc aattattgcc gttgtcgcgg cggtaaccac cggtgtattt ggcggcgttt
    10801 tgcgcgatat gttctgtaaa cgcattccgc tggtctttca aaaagagttg tatgccggtg
    10861 tctcatttgc ctccgccgtg ctgtacatcg cgctgcaaca ctatgtttct aaccatgatg
    10921 tggtgattat ctccaccctg gtattcggct tttttgctcg tttactggcg ctacgtctta
    10981 agctgggatt accggtcttt tactacagcc acgaaggtca ctaaggttca aaacctgtga
    11041 tctgctgggc agccagccaa ctgcccagct tcttgatttg cgcattttcc ttccattcaa
    11101 taacctgtct ggcgcgccca gatccagtcc ccggcagctg ctgccagaac tgctccgtgc
    11161 taaataaaag ttgcgaccag gatcgttcat cactggcatt aagcgccgcc cgggttagcg
    11221 gtattcccat tgccatcacc cagcgagtaa aaggctggtt acgagccaga ttaaactgat
    11281 gccataactg cgcactttta cttttcgcga tccccggcgt gttctgtaat tgctctggcg
    11341 ttaataaaag ccaggaaaag atatgctcaa agcgatgagt ctgatgcagc gcgcgccaac
    11401 cggcctcacc aatgccatcc agccccagaa cctgttttga ccccagccag actaagcgtg
    11461 aaatgaactg ttcctgacaa acatcagaag caaagtagca ggtcaacgag ttaaagcggt
    11521 tttctggtgg tgtcggtttt gtacgttctg caccgcgcca gaccacatca tcaatgcgag
    11581 gaataccctg accggcaagg ctgacgagga tctgatcacc aggcgcaata tcccactcct
    11641 gccagcgcct gacggaacca atattcaccc gctggacttt tttatcatcc agcatgacag
    11701 gtgcgagtga cgcaaccacc gatattttac cgctcttacc caccgcaaac tgaatcgcct
    11761 tcacttcggc aacctgagct acaggttgat atttccaggc caccagccac tccgcctggc
    11821 ccggtagcca atgacgggat tcgggttcct tagcccctcg cacaaccacg ccatcggtga
    11881 cgaagggtaa tttcgctttc caccactcat tgcgtacgcg cgcaacctca tcagcatttt
    11941 taaccgcacg ggtatacctc tgcgttagag taaaacctgc ggtagccagc tcctttaaac
    12001 gatcggtcat taactgcggt ccatccggcc atgcccagac aaaaacagcc agtgagttta
    12061 gcgtgtcgct attcccctgg cgcatcatca ggccagcaac ttttgcgcgg gcatttattc
    12121 cccccatttg ttgttggata tgcccctcgc gctggagaaa tatttccccc tgaagcgtac
    12181 tgttggctaa aggcccgcta acggtttgcg gcacagcgga aattaagcta actttctgcg
    12241 tccagtcctc gcctttcagg ccattaccgc gactgattgc tttgttcagt ttcccgtccc
    12301 gataaaccag ggttaccgca acgccatcaa cttttggctg cacccaaaga tcgctacgtt
    12361 ctcgcatcca cagacttaat gcgtttttat ccgccatttt gcgtacaccc gtatgggcaa
    12421 ccggatgcat aaccgcacca tttaaaggcg gcatcatcac gtcgcggggc tcgctcacaa
    12481 agcagcgttg ccactgcgta agacgggcgc ttaactgatc gtaaacaccg tcttccacct
    12541 cactttttcc ttccttccag tagtcatcgt cccactgttt tatttgctgt tgcaggcggg
    12601 aaatttcttc ctgtgctctg gatggcgacc aggccggaca gaccgcccac acagatgatt
    12661 gccagcacaa gatacttatt aatatcgcca tccatacttt catcttcacc tccgctgtag
    12721 tcaggctggc agatatacaa cgtggtgaag gcaaagccga gtggcaaaaa cggagtctgc
    12781 gaggacgctt cctgagaatc gtctttattg cagtaaatca caggcaaatg cggaaacagc
    12841 tacgcaaaac gcaacaactt tgcgcaaaaa gtgtgagcaa gggctacgtc acatggccgc
    12901 gccgtgtata ataagctcgt atgtaggctt tatttcgcta atcacatacg aaagatactc
    12961 atggctcaag gcacgcttta tattgtttct gcccccagtg gcgcgggtaa atccagcctg
    13021 attcaggctt tattaaaaac ccaaccgttg tatgacaccc aggtttctgt ttcacacacc
    13081 acacgccaac cgcgtcctgg tgaagtccac ggtgaacatt atttctttgt taatcatgat
    13141 gaatttaaag aaatgattag cagagatgcg ttcctcgaac acgcagaagt ttttggtaat
    13201 tactatggca cttcgcgtga ggccattgag caagtactgg cgaccggtgt cgatgttttt
    13261 ctcgatatcg actggcaggg cgcgcagcaa attcgccaga agatgccgca cgcgcggagt
    13321 atctttattt taccgccgtc caaaattgaa ctggaccgcc gtctacgcgg tcgcggtcag
    13381 gacagcgaag aggtcattgc aaagcgtatg gcgcaagctg ttgcagaaat gagccattac
    13441 gccgaatatg attatctgat tgtgaatgat gacttcgata ccgcgttgac cgatttgaag
    13501 accattattc gcgccgaacg tctgcgcatg agccgccaaa agcagcgtca tgacgcttta
    13561 atcagcaaat tgttggcaga ctgaacctga tttcagtatc atgcccagtc atttcttcac
    13621 ctgtggagct ttttaagtat ggcacgcgta actgttcagg acgctgtaga gaaaattggt
    13681 aaccgttttg acctggtact ggtcgccgcg cgtcgcgctc gtcagatgca ggtaggcgga
    13741 aaggatccgc tggtaccgga agaaaacgat aaaaccactg taatcgcgct gcgcgaaatc
    13801 gaagaaggtc tgatcaacaa ccagatcctc gacgttcgcg aacgccagga acagcaagag
    13861 caggaagccg ctgaattaca agccgttacc gctattgctg aaggtcgtcg ttaatcacaa
    13921 agcgggtcgc ccttgtatct gtttgaaagc ctgaatcaac tgattcaaac ctacctgccg
    13981 gaagaccaaa tcaagcgtct gcggcaggcg tatctcgttg cacgtgatgc tcacgagggg
    14041 caaacacgtt caagcggtga accctatatc acgcacccgg tagcggttgc ctgcattctg
    14101 gccgagatga aactcgacta tgaaacgctg atggcggcgc tgctgcatga cgtgattgaa
    14161 gatactcccg ccacctacca ggatatggaa cagctttttg gtaaaagcgt cgccgagctg
    14221 gtagaggggg tgtcgaaact tgataaactc aagttccgcg ataagaaaga ggcgcaggcc
    14281 gaaaactttc gcaagatgat tatggcgatg gtgcaggata tccgcgtcat cctcatcaaa
    14341 cttgccgacc gtacccacaa catgcgcacg ctgggctcac ttcgcccgga caaacgtcgc
    14401 cgcatcgccc gtgaaactct cgaaatttac agcccgctgg cgcaccgttt aggtatccac
    14461 cacattaaaa ctgaactcga agagctgggt tttgaggcgc tgtatcccaa ccgttatcgc
    14521 gtaatcaaag aagtggtgaa agccgcgcgc ggcaaccgta aagagatgat ccaaaaaatc
    14581 ctctcggaaa tcgaagggcg tttgcaggaa gcgggaattc cctgccgcgt cagtggtcgc
    14641 gagaagcatc tttattcgat ttactgcaaa atggtgctca aagagcagcg ttttcactca
    14701 atcatggaca tctacgcttt ccgcgtgatc gtcaatgatt ccgacacctg ttatcgcgtg
    14761 ctcgggcaaa tgcatagcct gtacaagccg cgtccgggtc gcgtgaaaga ctacatcgcc
    14821 attccaaaag cgaacggcta tcagtctttg cacacctcga tgatcggccc gcacggtgtg
    14881 ccggttgagg tccagatccg taccgaagat atggatcaga tggcggagat gggtgttgcc
    14941 gcgcactggg cttataaaga gcacggcgaa accagcacca ccgcgcaaat ccgcgcccag
    15001 cgctggatgc aaagcctgct ggagctgcaa cagagcgccg gtagttcgtt tgaatttatc
    15061 gagagcgtta aatccgatct cttcccggat gagatttacg ttttcacacc ggaagggcga
    15121 attgtcgagc tgcctgccgg tgcaacgccc gtcgacttcg cttatgcagt gcataccgat
    15181 atcggtcatg cctgcgtggg cgcacgcgtt gaccgccagc cttacccgct gtcgcagccg
    15241 cttaccagcg gtcaaaccgt tgaaatcatt accgcaccgg gcgctcgccc gaatgccgcg
    15301 tggctgaact ttgtcgttag ctcaaaagcg cgcgccaaaa ttcgtcagtt gctgaaaaac
    15361 ctcaagcgtg atgattctgt aagcctgggc cgtcgtctgc tcaaccatgc tttgggtggt
    15421 agccgtaagc tgaatgaaat cccgcaggaa aatattcagc gcgagctgga tcgcatgaag
    15481 ctggcaacgc ttgacgatct gctggcagaa atcggacttg gtaacgcaat gagcgtggtg
    15541 gtcgcgaaaa atctgcaaca tggggacgcc tccattccac cggcaaccca aagccacgga
    15601 catctgccca ttaaaggtgc cgatggcgtg ctgatcacct ttgcgaaatg ctgccgccct
    15661 attcctggcg acccgattat cgcccacgtc agccccggta aaggtctggt gatccaccat
    15721 gaatcctgcc gtaatatccg tggctaccag aaagagccag agaagtttat ggctgtggaa
    15781 tgggataaag agacggcgca ggagttcatc accgaaatca aggtggagat gttcaatcat
    15841 cagggcgcgc tggcaaacct gacggcggca attaacacca cgacttcgaa tattcaaagt
    15901 ttgaatacgg aagagaaaga tggtcgcgtc tacagcgcct ttattcgtct gaccgctcgt
    15961 gaccgtgtgc atctggcgaa tatcatgcgc aaaatccgcg tgatgccaga cgtgattaaa
    16021 gtcacccgaa accgaaatta atgttttatg aacccaacac gttatgcacg catctgcgaa
    16081 atgctcgcca ggcggcagcc tgatctgacc gtctgcatgg agcaggtcca caaacctcat
    16141 aacgtttctg cgattattcg taccgcagat gccgttggcg tacatgaagt tcacgccgtc
    16201 tggcctggta gccgcatgcg caccatggct tcggcagcgg cgggtagtaa cagctgggta
    16261 caggtgaaaa cacaccgcac cattggcgat gccgtcgctc atctcaaagg ccagggcatg
    16321 cagattctgg caacccatct ttctgataat gctgtcgatt tccgcgaaat tgattacact
    16381 cgcccgacct gcattttgat gggacaggag aaaacgggca tcacgcagga agcattggcc
    16441 ctggcggatc aggacatcat cattccgatg atcggcatgg tgcagtcgct gaatgtttcc
    16501 gttgcctcag ccctcattct ttacgaagcc cagcgtcagc ggcaaaatgc aggcatgtac
    16561 ctgcgtgaaa acagcatgtt gccggaagca gagcaacaac gcctgttgtt tgaaggcggc
    16621 tatccggtgc tggcgaaagt cgcaaaacgc aaaggcctgc cttatcccca cgtcaatcag
    16681 caaggcgaga tcgaagctga tgccgactgg tgggctacta tgcaggctgc agggtaagtg
    16741 ccatgaaagg tcgcctgtta gatgctgtcc cactcagttc cctaacgggc gttggcgcag
    16801 cacttagtaa caaactggcg aaaatcaacc tgcataccgt gcaggatcta ctcttacacc
    16861 ttcccctgcg ctacgaagat cgcacccatc tctaccccat cggagaacta ctgccgggcg
    16921 tttatgccac ggtggaaggc gaagtgctga actgcaatat ctccttcggc ggtcggcgga
    16981 tgatgacctg ccagatcagc gacggttccg gcatcctcac catgcgcttt ttcaatttca
    17041 gcgcggcaat gaaaaatagc ctggcgacgg gccgccgtgt actggcttat ggcgaagcaa
    17101 agcgcggtaa atatggtgcg gagatgatcc acccggaata ccgcgtgcag ggcgatctca
    17161 gcacgccaga attacaggaa acgctcacgc cggtttatcc aacaacggaa ggcgtaaagc
    17221 aggccacgct gcgtaaatta accgaccagg cgctggatct gctcgacacc tgcgccattg
    17281 aagaactcct gccgccggaa ctgtcacaag gaatgatgac gctaccggaa gcgttgcgca
    17341 ctttgcaccg cccgccaccg acgctacagc ttagcgatct ggaaaccggg cagcatccgg
    17401 cgcaacgtcg tctgattctg gaagaactgc tggcgcacaa cctcagcatg ttagccttac
    17461 gtgccggagc acagcgtttt catgcccagc cgctgagcgc caatgacacg ctgaaaaata
    17521 aactcctcgc cgccttaccg ttcaagccaa cgggcgcaca ggcacgcgta gtggcggaga
    17581 tcgagcatga tatggcgctg gatgtgccga tgatgcgcct ggtacagggc gatgtaggtt
    17641 ccggtaaaac gctggtcgcc gccctcgccg cgctgcgtgc gattgcccac ggcaaacagg
    17701 tggcactgat ggcaccaacc gaattactcg ccgagcagca cgccaataac ttccgcaact
    17761 ggtttgcacc gctcggtatc gaagtgggct ggctcgccgg taagcagaaa ggcaaagcgc
    17821 gactgtctca gcaggaagcc atcgccagcg gtcaggtgca gatgattgtc ggtacacacg
    17881 ccatcttcca ggaacaggtg cagtttaacg gcctggcgct ggtgattatc gacgaacagc
    17941 atcgttttgg cgtgcatcag cgtctggcat tgtgggagaa aggccagcag cagggcttcc
    18001 atccgcatca gttgatcatg accgccacgc cgatcccccg cacgctggca atgacggcgt
    18061 atgccgatct cgatacctcg gtgatagatg agctgccgcc aggccgcacg ccagtgacta
    18121 cggtcgctat tcctgatacc cgccgtaccg acatcattga ccgcgtgcgc cacgcctgca
    18181 taactgaagg tcgtcaggcg tactgggttt gtacgttgat tgaagagtca gagttactgg
    18241 aagcgcaggc ggcggaagct acctgggaag agttgaaact ggcgctacca gaattgaatg
    18301 ttggcctggt acacgggcgg atgaaaccag ccgagaaaca ggcggtgatg gcgtcgttta
    18361 aacaaggtga gctacatctg ctggttgcca ctaccgttat tgaagtcggt gttgatgtgc
    18421 ctaacgccag cctgatgatt atcgaaaacc ctgagcgtct gggtctggcg cagttacacc
    18481 agctgcgtgg gcgcgtaggt cgtggcgcgg tggcttctca ctgcgtgctg ctctacaaaa
    18541 cgccgctttc taaaacggcg caaattcgcc tgcaagtgct gcgcgacagt aacgacggtt
    18601 ttgtgattgc gcaaaaagat ctggagattc gcggccctgg cgaattgtta ggcacgcgtc
    18661 agacgggtaa tgctgaattt aaagtggcgg atttactgcg cgatcaggcg atgatcccgg
    18721 aagttcagcg cctggcgcga catattcacg aacgttaccc acaacaggca aaagccttga
    18781 tagaacgctg gatgccggag acagaacgtt actcgaatgc gtaaaaggcg gcggtgagaa
    18841 gaccgccgct tcatcggtta accggcaaaa atcggcaaca ttaaatacag cttaatcacc
    18901 agcgcattga cgatatcgat aaagaacgca ccgaccatcg gcaccaccaa aaacgccatg
    18961 tgcgacgggc caaagcgttc agtgatcgcc tgcatgttgg cgattgccgt tggcgttgca
    19021 ccgaggccaa aaccacagtg acccgcagcc agcactgccg catcgtagtt tttgcccatc
    19081 atgcgccagg taacgaagat ggcatacaac gccatgaaga tggtctgtac caccagaatc
    19141 gccagcatcg gcagtgccag cgaagccagc tcccacagtt tcagccccat caacgccatc
    19201 gccaggaaca acgacaagct tacgttaccc agcacggata ccgcacgctc aaagacgcgg
    19261 taaaagccca ttattgacag accgttgctc agaatcacgc caacaaacag tacacagacg
    19321 aaggtcggca gttcaaaagc agtgccagcc aaaagttgcg caacaatttt ccccaccgtc
    19381 aggcagatag caatcagcgc gatagtttca atcagcacca acgaggtgat catgcgtccc
    19441 acatccggct tttcaaacgc cgtcgggact tcctggtcat ccggaatacc gttcggcgtg
    19501 gtggagtgtt tcaccagata gcgcgccacc ggaccgccaa tcaagccgcc cagcaccaga
    19561 ccgaacgttg cacaggccat cgccacttcc gtcgcattgg tgaagccata acgttcaatg
    19621 aacaatttac tccacgcagc gcccgtaccg tgaccgccgg aaagcgtaat agaaccggcc
    19681 aacagcccca tcagcggatc aagccctaac aggctagcca taccaatacc aatggcattt
    19741 tgcatcacca acagaccaac aaccacaatc aagaagatgc caaccacacg cccaccggca
    19801 cgcaaactgg caatgttggc gttcaggcca atggtggcga agaaagccag cattaacgga
    19861 tcgcgcaggg acatatcaaa gttgacttcc cagcccatgc tttttttcag tactagtagc
    19921 gccagcgcca ccaacaaacc acccgcaaca ggttccggta tggtgtattt cttcaaaaag
    19981 gagacggaat ggaccaactt acgcccgagc agcaacgtca gcgttgcggc aacaagcgtt
    20041 gctaaagtat cgagatgaaa catagttact cctttgtatc cgcatcactc tttcatactc
    20101 gtcatacttc atcgcgcagt ggcaacttca tgaactatga cgggtatcga gttatgcttt
    20161 cctgggtcaa attctcagct gttaatgaac aaatggcatg gattttaagc agaaaaatac
    20221 caaaagttat ataaaaaagt gcatttatct catttaaata tttagatgag gatgtggcaa
    20281 acgtttgctt ttctctttcg gtcagctaaa atgcccgctt tgctaccacg ggattgtttt
    20341 caatgtctgt ttccaccctc gagtcagaaa atgcgcaacc ggttgcgcag actcaaaaca
    20401 gcgaactgat ttaccgtctt gaagatcgtc cgccgcttcc tcaaaccctg tttgccgcct
    20461 gtcagcatct gctggcgatg ttcgttgcgg tgatcacgcc agcgctatta atctgccagg
    20521 cgctgggttt accggcacaa gacacgcaac acattattag tatgtcgctg tttgcctccg
    20581 gtgtggcatc gattattcaa attaaggcct ggggtccggt tggctccggg ctgttgtcta
    20641 ttcagggcac cagcttcaac tttgttgccc cgctgattat gggcggtacc gcgctgaaaa
    20701 ccggtggtgc tgatgttcct accatgatgg cggctttgtt cggcacgttg atgctggcaa
    20761 gttgcaccga gatggtgatc tcccgcgttc tgcatctggc gcgccgcatt attacgccgc
    20821 tggtttctgg cgttgtggtg atgattatcg gcctgtcgct aattcaggtg gggctgacct
    20881 ccattggcgg cggttacgca gccatgagcg ataacacctt cggcgcaccg aaaaatctgc
    20941 tgctggcagg cgtggtttta gccttaatta tcctgcttaa ccgtcaacgt aacccttact
    21001 tacgcgtggc ctcactggta attgcgatgg cggccggata tgcgctggcg tggtttatgg
    21061 gcatgttgcc agaaagcaac gaaccgatga cgcaagaact gattatggtg ccaacgccgc
    21121 tctattacgg tcttggcatt gaatggagtc tgctgctgcc gctgatgctg gtctttatga
    21181 tcacttcgct ggaaaccatt ggcgatatca cggcgacctc tgacgtttcc gaacagccag
    21241 tgtccggtcc gctgtacatg aaacgcctga aaggcggcgt gctggcaaac ggcctgaact
    21301 cgtttgtttc ggcggtgttt aacaccttcc cgaactcctg cttcgggcaa aacaacggag
    21361 tgatccagtt gactggtgtt gccagccgct atgtcggttt tgtcgtcgcg ctgatgttga
    21421 tcgtgctggg tctgttcccg gcagtgagcg attttgtaca acacattcca gaaccggttc
    21481 tgggcggcgc aacgcttgta atgtttggca ccatcgccgc ctccggtgtg cgtatcgttt
    21541 ctcgtgagcc gctgaaccgt cgggcgattc tgattatcgc gctgtcgctg gcggttggtc
    21601 tgggcgtgtc tcagcagccg ctgattttgc agtttgcccc tgaatggctg aaaaacctgc
    21661 tctcctccgg gatcgccgcg ggcggtatta ctgccatcgt gctgaatctg attttcccac
    21721 cagaaaaaca gtaattcact ggcggcggta agataattac cgccgcagtc tcacctcttt
    21781 caccattccc gccttgagga ttgcgcgtaa atcgtgcata actcccttat gtgcatttca
    21841 cgggatggaa gaccatgaaa tttattggga agctgcttct ctacattctc atcgctctgt
    21901 tagtggtgat cgctggcctc tattttcttc tgcaaacccg ctggggagca gaacatatca
    21961 gcgcatgggt ttccgagaat agcgactatc atctggcctt cggggcgatg gatcaccgtt
    22021 tttccgcgcc atctcatatc gtgctggaga atgtcacgtt tggtcgtgat ggtcagcccg
    22081 cgcccctggt ggcaaaaagt gtcgacattg cgctaagcag tcgccaactg accgaaccac
    22141 gccatgtcga taccatcctg ctggaaaacg ggacgctgaa tctcaccgac cagaccgcgc
    22201 cgctaccgtt caaagccgat cgtctgcaac tgcgtgatat ggcgtttaat agcccgaata
    22261 gcgaatggaa actgagcgcg cagcgggtaa atggcggcgt ggttccgtgg tcaccagaag
    22321 ccggtaaagt gctgggtacg aaggcgcaga ttcagtttag tgccggatcg ctttcgctca
    22381 atgatgttcc tgccaccaat gtactgattg aaggcagtat tgataacgat cgcgttacgc
    22441 tgactaacct gggtgccgac atcgcccgcg ggacattaac cggaaacgcg cagcgtaacg
    22501 ccgacggcag ctggcaagtg gaaaatctgc gcatggcgga tatccgtcta caaagcgaaa
    22561 aatcgctaac cgacttcttt gcgccattac gctctgtccc gtcgttgcag attggtcgct
    22621 tggaagtcat cgatgctcgt ctgcaaggtc cggactgggc ggtgaccgac ctcgatctca
    22681 gcttgcgcaa catgaccttc agtaaagatg actggcagac acaggaaggc aaactgtcga
    22741 tgaacgccag cgagttcatt tatggttcgc tgcatttatt tgacccgatt ataaacgcgg
    22801 aattttcccc gcagggcgta gcgctgcgcc agttcaccag ccgctgggaa gggggtatgg
    22861 tcagaacgtc agggaactgg ctgcgtgacg gtaaaacgtt gatccttgat gatgcggcaa
    22921 ttgccgggct ggaatatacc ttgccgaaaa actggcaaca gttgtggatg gaaacgacac
    22981 ccggttggtt aaacagcctg caactgaaga gatttagcgc cagccgcaat ctgatcattg
    23041 atatcgaccc tgacttcccg tggcagctca ccgcgctcga tggttacggt gccaacctga
    23101 cgctggttac cgatcataaa tggggcgtct ggagtggctc ggcgaatctg aatgccgccg
    23161 ccgcgacatt caatcgtgtt gatgttcgtc gcccgtcgct ggcgctgacc gccaacagca
    23221 gcacggtgaa tatcagcgaa ctgagtgcat ttactgaaaa aggcattctg gaagccaccg
    23281 caagtgtttc acaaacgcca caacgtcaga cccatatcag cctgaatgga cgcggtgtgc
    23341 cggtgaatat tttgcaacaa tggggatggc ctgaattaca gttgactggc gacggcaata
    23401 ttcagcttac cgccagtgga gatattcagg ccaatgcccc gctgaaacct acggttagcg
    23461 ggcaattgca tgccgtgaac gccgcaaagc agcaagtgac tcaaaccatg aatgcgggcg
    23521 tcgtttccag cggtgaagtt acatcgacgg agccggtgcg gtaaacatca atctcgccgg
    23581 gtacgactca gacgtacccg gcattccgtc aatagattac aacgtaattg tcagcgcatt
    23641 cccttgaggc gtcaccacca gcccctgctc actttcagcc tgcgaaccgc cttgcagacc
    23701 atttactttc acaacattgc gcaagcacag cgtccagttc ttcgcctcgc ccgcaccagt
    23761 cacagtaatc gtgttgccag tacgtgctgc ttttaaagta aagatcaccg atccgtcagc
    23821 agcaggcact tcacagacgg cttcatgccc gtcttgcaga ttaaagaggt ggaatgccgt
    23881 gccttcgtgc cacacgtaat cgggacgttg atcgttgttg cccagcgcca gtagagtgtt
    23941 atcacgcaca taaacgggca gactcaggaa gccgtgctgc tgtttatgcc agcgactacc
    24001 gtcgagttca tcgttgtgcc acaggtgtgt ccagcgacct tccggcaggt agaactgcac
    24061 atcgcccgct tcagtgaaca ccggcgcaac catcacgttg tcgcctaaca tgtattgacg
    24121 gtcaaggtaa tcacaagccg gatcgtccgg gaactccatc atcatggccc gcatcatcgg
    24181 cgtaccccgc gcgttcgcac gcgcagcttc acgatacaga tacggcatca tgcggcattt
    24241 cagttgcgtg aagaagcgca ccacatcaca ggactcatca tcgtaggccc acggcacacg
    24301 ataagatttg ctaccgtgta aacggctatg gctggagagc aaaccaaacg cgcaccagcg
    24361 tttgtaaacg tgcgccggag cggtattttc aaagccgccg atatcgtggc tccagaagcc
    24421 aaaacctgaa aggccaatag acaaaccacc gcgcaggctt tccgccattg attcgtagtt
    24481 agcgtaacaa tcgccacccc agtgtaccgg gaatttctgc gcaccgacgg aggccgagcg
    24541 ggcaaacaag acagcttctt cctcaccaac ggtgtccttg agcacgttcc acaccagttc
    24601 gttgtagatg tacgcataat ggttatgcat tttctgcgga tcggaaccgt caaaccactg
    24661 aacatcagtt gggatacgtt cgccaaagtc ggtcttaaag caatcaacgc ccatcgcgac
    24721 cagacctttc agtttgtcgg cgtaccattt gcaggcatcc ggattggtaa agtcataaat
    24781 cgccagacct ggctgccatt tatcccactg ccatagcgaa ccgtccgggc gtttgagtaa
    24841 atagcctttc tcttgtaact ctttaaagac gggggatttt tgaccgatat aggggttaat
    24901 ccagacgcag attttcagcc ctttcgcttt caggcggcgg atcatccctt ccgggtcagg
    24961 gaaagtcagc gggtcccact caaaatcgca ccactggaag gctttcatcc agaaacagtc
    25021 aaagtggaaa acatgcagcg gcagattgcg ttccgccata ccatcgataa agctgtttac
    25081 cgtcgcttcg tcgtagttgg tggtaaatga agtggttagc cacaggccga aggaccacgg
    25141 gggcggcagc gccggacgac cagtaaagcg ggtataacga tcaagtaccg ctttcggcgt
    25201 cgggccgtcg ataacaaagt attcgagata ttcactctca acgctgaact gcactttgga
    25261 gactttctcc gatcccactt caaaagagac acactgggga tgattgacca gtaccccata
    25321 accacggtta gtcatgtaga acgggatatt tttatacgcc tgttcagtac ttgtgccgcc
    25381 gtcccggttc caggtctcta ccgtctggcc attgcgcacc agggcagtaa agcgctctcc
    25441 cagaccgtaa actgtttcgc caacgccaag atcaagccgc tcaaacatat aattgcgttg
    25501 attattcgtg tcctgcacgt agccattatt tttcacctga ctaccggtaa tacgttcgcc
    25561 gttgcgcaga aaatccagtg accagaactc acctttgctg acacgcgcgc ttaagttgcc
    25621 acttttaaac tcagcataac gttctgtgtt ttcgattgtg accttcacgt cctgcaaaat
    25681 attgagcgga taatgaggac cgttattcag cgccccctga aaatactcaa tccgcacacc
    25741 gacaatacct tcctgtgggg agaaaaagcg cagcgtaaat aaaggcgtat caagctgcca
    25801 ggtacgttca cgcacatcac ggggggcagc atagaccacc atttcattat cctgctgttc
    25861 aacctcgaat acctgaagcg ggtgaatcaa attgaggcca ggttgaatca accagtttcc
    25921 atcgctaatt ttcattgccg ttccttagtt ctgcacttct tgagaggtga attgttgctg
    25981 gcaacggcgt ttaccctgag ccagctgttc cataacggtt ttcagattgt gcgtcgtgag
    26041 tgagtagtag cgtttagcga taatcgcgct cagcaaataa cagatcgccg gaacaatcgt
    26101 gaatagcgca ataatgatgc taatcgtggc gctgttctgc gctttttctg ccgcatcata
    26161 tccgccataa gccagcatcc agccgataag agcgccgccg aaggccaacc ccagtttgag
    26221 cacaaacagc gtgccagcaa aactgatccc ggtcaggcgt ttaccattgc accactcgcc
    26281 gtagtcgacg gtatcgaaca tcattaccca ctggataggt gtcaccagtt gatgcaacac
    26341 accaatcacg aagatgaaga caaacatagt gatgctggcc tgcatgggaa caaagaacat
    26401 cgcgaggcta atcactgcca gcagggcgtt cgtccaccag aagatagtga ctttacattt
    26461 ccagtcggtc agaggttttg ccagtgcgga accaatcagg ttacccacgc aataagtggt
    26521 gagaaaagcg acaaacactt ccggcgtgcc caaaatccat gtgacgtaat acatcatcgc
    26581 cccaccgcgt acgcacaccg ccaggatatt gaaaatggtt agtaaaccga caatccgcca
    26641 ctggtcgttt tgccagatat cacgtaaatc ttcccgcata gacgttgttg taggtggtgc
    26701 ttcaacgcgt tctttagtgg tgaagaaaca aaatgccagc atcatgaatg ccaccacgga
    26761 aaggaccacg ataccgccct ggaaaccgag tggtttatta tcaccgccaa ttaaattaac
    26821 cagtggcatc atcagaacag tagaaagcat gcctcccgcg gtcgccagca caaaacgcca
    26881 ggattgcagc gagatacgct gagtcgggtc attggtgatt acaccaccca atgcgcagta
    26941 agggatattg acgacggtat aaagtaaggt aagttagggt gtaagtaatt gctgcataga
    27001 tcattttgcc gttcatactg agatctggcg tgctataggc cagtacacag acgatcccga
    27061 atggcagtgc gccaaacagt acccacggac gaaatttacc ccagcgagag cgcgttcgat
    27121 cggccaacaa ccccatgcaa ggatcggaaa tcgcatccag tgcacgagcg accaaaaaca
    27181 tggttccgac aaatccggca ggaatgccaa aaatatcggt ataaaagaac atcatatata
    27241 acattacgtt atcgaaaata atgtggctgg cggcgtctcc catgccataa ccaattttct
    27301 ctttaacgga caacacttca ctcttcatct gcttttcctt gatgctatag gggtgctgag
    27361 accgataacg cattttgtaa accatccctg tgagagcgaa tattccgata tctggttatt
    27421 aatttatgat tcttgtttta tgtgatcgtg gtagcgttaa ttccgctcat atatcattgt
    27481 aaaatatggg ttttatataa attataatgc tttcgtgata atacgctgcg tttattaggc
    27541 ggaaaaaact gatctggggg atgtagaaac tcaaggaagt agctataatg cgccccgcct
    27601 ccatgtagca atcgaggcgc ggaagatcgt cgtctccggt gaggcggctg gacttcaaat
    27661 ccagttgggg ccgccagcgg tcccgggcag gttcgactcc tgtgatcttc cgccagttta
    27721 cctcttctga actctcccaa tgtcaataaa tctcttctaa tcccttgtat atcagtattt
    27781 atttgtatcc tgaggtcaac ctacttcacc ccgtatcaag tttcttttgg gggtacttta
    27841 gggggtgtta tttcctattg aaatacaagc acccccataa taggaaaaat gtgatacata
    27901 acaacggctt ttaacaatat ctttgttata caaggggtgt attgggggta tcaggttagt
    27961 tactggcaga aatccccctc aggaacatga attcatcaaa agccggagga tgtcatggca
    28021 ttaaccgata tcaaagtgaa aaccgcaaaa cccaaagaca aaccctataa gcttgctgat
    28081 ggcggcggta tgtatctact gattaacact aatggttcta aatactggcg catgaagtac
    28141 cgctttgctg gtaaagaaaa gatgctttct attggcgtat accccgatgt aacgctagcc
    28201 gatgctcgcg agaaacgcag tgaagccagg aaactcctcg ctgctggagg cgatccaggg
    28261 gaggcaaaaa aggaagaaaa aatcgcgcag caaatgagtc tgaaaaatac gttcgaggct
    28321 attgctcgcg aatggcacca gttaaaagct gatcggtggt cattacgcta tcgtgatgaa
    28381 atcattgata cctttgaaaa agacattttt ccctacattg gcaagcgccc tattgccgaa
    28441 atcaaaccaa tggagttact ggaagcactg cgtaaaatgg aaaaacgtgg agctctggag
    28501 aaaatgcgca aggtccgcca gcgctgtggt gaggtgttcc gctatgcaat tgttactggc
    28561 cgggcagact ataacccagc ccccgatctt gccagtgctt tagctacacc gaaaaaagta
    28621 cattttccct ttcttactgc taatgaactt ccccacttcc tcaacgatct ggcgggttat
    28681 accggaagta taatcactaa gacagctact cagatcatta tgttaaccgg cgtacgaaca
    28741 caagaactgc gttttgcacg ctgggaagat atcgattttg agacaaaatt gtgggagatc
    28801 ccggcagaag taatgaagat gaagcgacct catattgtgc cgctctctga gcaggtcatt
    28861 atgctattta agcagcttga accaatctca aaacatcatc ccctagtctt tatcggtaga
    28921 aatgatcccc gcaaaccaat tagtaaggaa agcattaacc aagtaattga attacttgga
    28981 tataagggaa gactcacagg gcacggtttc agacacacca tgagtacaat tctgcatgaa
    29041 caaggcttta attccgcctg gattgaaatg cagttagctc at
//
LOCUS       DADMEQ010000044        28463 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.044, whole genome shotgun sequence.
ACCESSION   DADMEQ010000044 DADMEQ010000000
VERSION     DADMEQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.044"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..1198
                     /gene="pdeC"
                     /locus_tag="JNZ05_001931"
     CDS             <1..1198
                     /gene="pdeC"
                     /locus_tag="JNZ05_001931"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HAY8565175.1"
                     /translation="IYYYRDTPFFSGYKMTYMQRGNYVAVINPLFWSEVMSDDPTLQW
                     GVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATIYSTKRPIAAIVATSY
                     QRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCLYYQP
                     IIDIKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAEKEGMIEQITDYVIDNVFRDLGA
                     YLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEKMT
                     PIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAEH
                     IIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLPAR
                     ELTRGQ"
     gene            complement(1201..1524)
                     /gene="soxS"
                     /locus_tag="JNZ05_001932"
     CDS             complement(1201..1524)
                     /gene="soxS"
                     /locus_tag="JNZ05_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HAY8565176.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1610..2074
                     /gene="soxR"
                     /locus_tag="JNZ05_001933"
     CDS             1610..2074
                     /gene="soxR"
                     /locus_tag="JNZ05_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HAY8565177.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2778..4127
                     /locus_tag="JNZ05_001934"
     CDS             2778..4127
                     /locus_tag="JNZ05_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY8565178.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4278..5927
                     /locus_tag="JNZ05_001935"
     CDS             4278..5927
                     /locus_tag="JNZ05_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HAY8565179.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(6081..7373)
                     /locus_tag="JNZ05_001936"
     CDS             complement(6081..7373)
                     /locus_tag="JNZ05_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY8565180.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(7551..9200)
                     /gene="actP"
                     /locus_tag="JNZ05_001937"
     CDS             complement(7551..9200)
                     /gene="actP"
                     /locus_tag="JNZ05_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HAY8565181.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLITERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVMLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLRLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(9197..9511)
                     /locus_tag="JNZ05_001938"
     CDS             complement(9197..9511)
                     /locus_tag="JNZ05_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HAY8565182.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(9711..11669)
                     /gene="acs"
                     /locus_tag="JNZ05_001939"
     CDS             complement(9711..11669)
                     /gene="acs"
                     /locus_tag="JNZ05_001939"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HAY8565183.1"
                     /translation="MSQIHKHTIPANITDRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPKAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            12062..13498
                     /gene="nrfA"
                     /locus_tag="JNZ05_001940"
     CDS             12062..13498
                     /gene="nrfA"
                     /locus_tag="JNZ05_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HAY8565184.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALEEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            13543..14109
                     /gene="nrfB"
                     /locus_tag="JNZ05_001941"
     CDS             13543..14109
                     /gene="nrfB"
                     /locus_tag="JNZ05_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HAY8565185.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            14106..14777
                     /gene="nrfC"
                     /locus_tag="JNZ05_001942"
     CDS             14106..14777
                     /gene="nrfC"
                     /locus_tag="JNZ05_001942"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HAY8565186.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCEHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            14774..15730
                     /gene="nrfD"
                     /locus_tag="JNZ05_001943"
     CDS             14774..15730
                     /gene="nrfD"
                     /locus_tag="JNZ05_001943"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HAY8565187.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEYDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAVCGASLVGVLMLRFFILYAGQLTVA"
     gene            15750..17468
                     /locus_tag="JNZ05_001944"
     CDS             15750..17468
                     /locus_tag="JNZ05_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY8565188.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLAAFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            17461..17844
                     /gene="nrfF"
                     /locus_tag="JNZ05_001945"
     CDS             17461..17844
                     /gene="nrfF"
                     /locus_tag="JNZ05_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HAY8565189.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWKFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            17841..18437
                     /gene="nrfG"
                     /locus_tag="JNZ05_001946"
     CDS             17841..18437
                     /gene="nrfG"
                     /locus_tag="JNZ05_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HAY8565190.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            18779..20092
                     /gene="gltP"
                     /locus_tag="JNZ05_001947"
     CDS             18779..20092
                     /gene="gltP"
                     /locus_tag="JNZ05_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HAY8565191.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWSLIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(20170..20859)
                     /locus_tag="JNZ05_001948"
     CDS             complement(20170..20859)
                     /locus_tag="JNZ05_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY8565192.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDQRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(20953..23100)
                     /gene="fdhF"
                     /locus_tag="JNZ05_001949"
                     /pseudo
     CDS             complement(20953..23100)
                     /gene="fdhF"
                     /locus_tag="JNZ05_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(23298..24764)
                     /gene="mdtP"
                     /locus_tag="JNZ05_001950"
     CDS             complement(23298..24764)
                     /gene="mdtP"
                     /locus_tag="JNZ05_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HAY8565193.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(24761..26812)
                     /gene="mdtO"
                     /locus_tag="JNZ05_001951"
                     /pseudo
     CDS             complement(24761..26812)
                     /gene="mdtO"
                     /locus_tag="JNZ05_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
     gene            complement(26812..27843)
                     /gene="mdtN"
                     /locus_tag="JNZ05_001952"
     CDS             complement(26812..27843)
                     /gene="mdtN"
                     /locus_tag="JNZ05_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HAY8565194.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGDDSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAALEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(27862..28137)
                     /locus_tag="JNZ05_001953"
     CDS             complement(27862..28137)
                     /locus_tag="JNZ05_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565195.1"
                     /translation="MPTVLSRMAIHLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(28346..>28463)
                     /locus_tag="JNZ05_001954"
     CDS             complement(28346..>28463)
                     /locus_tag="JNZ05_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565196.1"
                     /translation="ADLVKAKRAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         6484 a         7207 c         7643 g         7129 t
ORIGIN      
        1 tatctattat taccgcgata cgcctttttt ctctggctat aaaatgacct atatgcagcg
       61 gggaaattat gtggcagtta tcaaccctct cttctggagt gaagtgatgt ctgatgaccc
      121 gacattgcaa tggggtgtgt atgatacggt gacgaaaacc tttttctcgt taagcaaaga
      181 ggcctcggca gcaacgtttt ctccgctgat tcatttgaag gatttaaccg tacaaagaaa
      241 tggctattta tatgcgacaa tttattcgac aaaacgccca attgcagcca ttgttgcgac
      301 ttcatatcaa cgtcttataa cccattttta taatcatctt atttttgcgt tgcccgccgg
      361 tattttgggg agtcttgttc tgctattact ctggctacgt attcgacaaa actatttatc
      421 tcccaaacgt aaattgcaac gcgccctcga aaaacatcaa ctttgtcttt attaccagcc
      481 aataatcgat atcaaaacag aaaaatgtat cggcgctgaa gcgttgttac gttggcctgg
      541 tgagcagggg caaataatga atccggcaga gtttattccg ctggcagaaa aggaggggat
      601 gatagaacag ataactgatt atgttattga taatgtcttc cgcgatctgg gcgcgtacct
      661 ggcaacacat gccgatcgct atgtttctat taacctgtcg gcctccgatt ttcatacgtc
      721 acggttgata gcgcgaatca atcagaaaac agagcaatac gcggtgcgtc cgcagcaaat
      781 taaatttgaa gtgactgagc atgcatttct tgatgtcgaa aaaatgacgc cgattattct
      841 ggctttccgc caggctggtt acgaagtggc aattgatgat tttggtattg gctactctaa
      901 cttgcataac cttaaatcat tgaatgtcga tattttgaaa atcgataaat cgtttgttga
      961 aacgctgacc acccacaaaa ccagtcattt gattgcggaa cacatcatcg agctggcgca
     1021 cagcctgggg ttaaaaacga tcgctgaagg cgtcgaaact gaggagcagg ttaactggct
     1081 gcgcaaacgc ggcgtgcgct attgccaggg atggtttttt gcgaaggcga tgccgccgca
     1141 ggtgtttatg caatggatgg agcaattacc cgcgcgggag ttaacgcgcg ggcaataaaa
     1201 ctacaggcgg tggcgataat cgctgggagt gcgatcaaac tgccgacgga aaacgcggga
     1261 gaaggtctgc tgcgagacat aacccaggtc cattgcgata tcaaaaatcg gacgctcggt
     1321 ggtgcgcaac tcaacggcgg ccagtaacag gcggcgttgg cgaatgtaat cgccaagcgt
     1381 ctgatgcgtc accgtgcgga acatccgttg caagtaccac tttgaatagc ctgatttttt
     1441 tgcgacgaca tcaatgttaa gcggctggtc aatatgcacg tcaatccatg cgataagatc
     1501 ctgaataatt ttctgatggg acataaatct gcctcttttc agtgttcagt tcgttaattc
     1561 atctgttggg gagtataatt cctcaagtta acttgaggta aagcgattta tggaaaagaa
     1621 attaccccgc attaaagcgc tgctaacccc tggcgaagtg gcgaaacgca gcggtgtggc
     1681 ggtatcggcg ctgcatttct atgaaagcaa agggttgatt actagtatcc gtaacagcgg
     1741 caatcagcgg cgatataaac gtgatgtgtt gcgatatgtc gcaattatca aaatcgctca
     1801 gcgtattggc attccgctgg cgaccattgg tgaagcgttt ggcgtgttgc cagaagggca
     1861 tacgttaagt gcgaaagagt ggaaacagct ttcgtcccaa tggcgagaag agttggatcg
     1921 gcgcattcat accttagtgg cgctgcgtga cgaactggac ggatgtattg gttgtggctg
     1981 cctttcgcgc agtgattgcc cgttgcgtaa cccgggcgac cgcttaggag aagaaggtac
     2041 cggcgcacgc ttgctggaag atgaacaaaa ctaaagcgcc acaaggacaa gctgactcat
     2101 cgttaactat gaggccgggg tgagggtaaa tatttgcact ggtgctgcct gttcccctca
     2161 ccctaaccct ctccccgaag gggcgagggg actgatcgag ctcttttgga attatccgca
     2221 aacacaaaac taaagcgcca caagggcgct ttggtttgtt ttccggtctt tgtctttctc
     2281 tctatcccgc tggaacacag gagggtttcc cccgacgtca acacacctca ttcgagcacg
     2341 tggtggaggt tccggttggt gttgatgctt taattgtatg tcaccgacgt ttcttcgcca
     2401 gtgtaaaagt atacttttta gccgcaatat ttttgtcatc tcagacgatt ttttatcgca
     2461 atccagaacg gtatacggct cgataacgct gcaatcttgc acatcgacga taacgtttgc
     2521 gcatcaattg cctggttttt catcgtcaag acaataaaag agaaaaaagc agcaaacttc
     2581 ggttgaaaaa gccgctatga tcgccggata atcgtttgct tttttaccac ccgttttgta
     2641 tgcgcggagc taaacgtttg cttttttgcg acgcaccaaa ttgccgcaaa cctggagcag
     2701 gaagataacg tttcgctggc aggggattgt ccgccacgca tcttgacgaa aattaaactc
     2761 tcaggggatg ttttcttatg tctacgccat cagcgcgtac cggcggttca ctcgacgcct
     2821 ggtttaaaat ttcacaacgt ggaagcactg tccgtcagga agtggttgcc gggttaacaa
     2881 cgtttctggc gatggtctac tcggtcatcg tcgttccagg tatgttgggt aaagcaggct
     2941 tcccgcctgc ggcagttttc gttgcaacct gtctggttgc cggactcggt tctatcgtga
     3001 tgggtctgtg ggctaatctg ccgttggcga ttggttgcgc catctccctg acggcgttta
     3061 ccgcattcag cctggtgctg gggcaacata ttagcgtacc tgtcgcgctg ggtgccgtgt
     3121 tcctgatggg ggtgctgttt acggtaattt ctgccacggg tatccgtagc tggattttgc
     3181 gcaacttgcc gcacggtgtg gcgcacggca cggggatcgg tatcggtctg ttcctgctgc
     3241 tcattgccgc taatggtgtc ggtctggtga ttaaaaaccc gcttgatggt ctgcccgttg
     3301 cgctgggtga tttcgcgacc ttcccggtga ttatgtcact ggtaggtctg gcggtgatca
     3361 tcggcttgga aaaactgaaa gtccctggtg gcattctgct gaccattatc ggtatctcaa
     3421 ttgtcggttt gatcttcgat cctaacgtcc atttctccgg cgttttcgcc atgccttcat
     3481 tgagcgatga aaacggcaat tcactgattg gcagcctgga cattatgggc gcgctgaatc
     3541 ctgtagtcct gccaagcgtt ctggcgctgg tgatgacggc agtatttgat gccaccggaa
     3601 ctatccgcgc tgtcgccggc caggcgaacc tgctggataa agatgggcag attatcgacg
     3661 gcgggaaagc actgaccact gactccatga gcagcgtttt ctctggcctg gtgggtgcag
     3721 ctccggcagc ggtatacatc gagtctgcgg cgggtacggc ggcgggcggt aaaaccgggt
     3781 tgacggctat caccgttggc gtgctgttcc tgctgattct gttcctctct ccgctttctt
     3841 acctcgttcc ggggtatgca acggctccgg cgctgatgta cgttggcctg ctgatgctga
     3901 gcaacgtggc gaaaatcgac tttgctgatt ttgttgatgc gatggcgggt ctggttacgg
     3961 cggtattcat cgtgctgacc tgtaacatcg taacaggcat catgatcggc ttcgcgactc
     4021 tggtgattgg tcgtctggtt tccggtgaat ggcgcaagtt gaacatcggt acggtcgtta
     4081 tcgccgtggc gctggtgacc ttctatgcgg gtggctgggc tatctaatct ttcatcggat
     4141 tctgaaaacg ggtggcaatg gctgcccgtt tttattttct ccgcacattg tgtgagcttt
     4201 tgcgatattc tgaaaaaatg agaattcagg cataacgtct ttctcagatc ataaaaaaca
     4261 tcgcaaacag ggaacgcatg gaaatcttct tcaccatact gataatgacc ctcgtggtct
     4321 cgctgtccgg ggtggtcact cgtgtcatgc cctttcagat cccgcttccg cttatgcaaa
     4381 tcgccatcgg tgcgctactg gcgtggccga cgtttggttt gcatgtggag tttgatcctg
     4441 aactcttttt agtcttgttt atcccgccgt tgctgttcgc tgatggctgg aaaacgccga
     4501 cccgtgaatt tctcgaacat ggtcgagaga ttttcggcct cgcgctggcg ctggtggtgg
     4561 tcaccgtggt cggcattggc ttccttattt actgggtggt gccgggcatt ccgctgatcc
     4621 ccgcctttgc gctggcggcg gtactttctc cgaccgatgc tgtggcgctc tccgggattg
     4681 ttggcgaagg gcgcatcccg aaaaaaatca tgggcatttt gcagggcgaa gcgttgatga
     4741 atgacgcttc cggcctggtg tcgttgaagt ttgccgtggc agtggcgatg gggacgatga
     4801 tcttcactgt cggcggtgcg acggtcgaat ttatgaaagt agccattggc ggtattcttg
     4861 ccggttttgt ggtgagctgg ctgtatggtc gctcgctgcg attcctcagc cgctggggcg
     4921 gtgatgaacc cgcgacgcag atcgttctgc tgttcttgct gccattcgct tcttatctga
     4981 ttgccgaaca tattggcgtt tcgggcatcc tcgctgcggt tgccgccggg atgaccatca
     5041 cccgctccgg tgtgatgcgc cgtgcgccac tggcaatgcg cctgcgcgca aacagcacct
     5101 gggcgatgct ggaatttgtc tttaacggca tggtgttcct gctgttaggt ctgcaactgc
     5161 cgggtattct ggagacgtcg ctgatggcgg cagaaatcga ccctaacgtc gaaatctgga
     5221 tgctgtttac cgatattatt ctgatatatg cggcgctgat gctggtccgt ttcggctggc
     5281 tgtggacgat gaaaaagttc agcaaccgct tcctgaagaa gaagccgatg gagtttggtt
     5341 cgtggaccac acgagaaatc ctgatcgcgt ctttcgctgg ggtgcgtggg gcgatcactc
     5401 tggccggtgt gctctcaatc ccgctgctct tgccggatgg taacgtcttc ccggcgcgct
     5461 atgagctggt gttcctggcg gctggcgtca ttctcttctc gctgtttgtc ggcgtggtga
     5521 tgttgcctat tctgctacaa cacattgaag tcgcggacca ttcgcaacaa ttgaaagaag
     5581 aacgtattgc gcgagcggca acggcagaag tggcgattgt ggcgatccag aaaatggagg
     5641 agcgtctggc ggcggatacc gaagagaata tcgataacca gttgcttacg gaggtcagtt
     5701 ctcgcgtcat tggtaacctg cgtcgtcgcg ccgatggacg taacgacgtt gaaagttccg
     5761 tgcaggaaga gaaccttgag cgtcgcttcc gtctggcggc attgcgttct gaacgtgctg
     5821 aactgtacca cctgcgcgcc acgcgggaga tcagcaacga aacgctgcaa aaattactgc
     5881 acgatctcga tttgcttgaa gcgttgctaa ttgaggaaaa tcagtaagcc gaaagcgacg
     5941 taaccaggcc cgaggtaatc tgaaaaaggt gcaatctttt gcacctttaa caggttagct
     6001 cgggcgaaca ttcatgattc taaaacaaat aagttatctc tcttgtaaag tcaattcatt
     6061 ggctgttctt cgacttaacc tcaatctgcg tgttgcataa tatctgcagt aatcttcata
     6121 gcgactgcat taatattatc ctcagtttgt gacatgccag tatgagaggt acaatcttct
     6181 tcaaagaatt tctgtagtgt taccagggta tcactatcaa tggggatatt ttcctcccca
     6241 cggagaatgg aaatgcccat tccctggacg ctagcctcat cgaaattaac ccgatagaga
     6301 cgggtattaa acatacaggc ttttgttaaa atagaacagt tgaaatggca gttgcttaaa
     6361 ttggcattat taaaattaac attagccata ttagagccgg tacatataac catttcgagg
     6421 aatgtatttg tcagccacgt acaaaagagt ttgctatcaa ttaagtttat attctggagg
     6481 attgctttgt tcagattgac gcgagacaga tcagcatgtt caaagttaat taaatcgagt
     6541 ttagagaaac taaggtcggc tttaaataaa ttaacatatg gcgctattac tttataaaga
     6601 tttacctcag ccatgtacgc catataaaaa tttgaatagt caaggaaggt atttttaata
     6661 ttggctctat tcattgctgc attgtagaat tgaacatgca tagcggagca tgcatttaga
     6721 gttccgtcgg ccatttttat ctgagcgaaa tttgagtcat cgagaataca attatcaaaa
     6781 agtatttttt ccattatggc gtaataaaaa ttcgttttat acatattaca gtcctggaaa
     6841 atagcgtttt gtagtctgca acctgcaaaa ttcgaattgg ataaatcttc atctttaatc
     6901 acgataccct tcaggctaag gtgtgaaaaa tcgtaaccac catttactat ctgtggaatt
     6961 tcgatagtat tattatggac ggcgacatta tttataaaat ataccacctt aatggataca
     7021 tccttttctt tactaaggta aactcttctc gtaattctat cgtcacattt attatttgca
     7081 tataacgcta tatctataaa atatttatgt attttcctgt ccgtttcttt cgtgattttt
     7141 cctgatggta aactatcata aattcttttc attttctgaa tcaagcctat cactttttca
     7201 tgatcatcta ttttgaaaag agagagggct ttattgtcaa ttaacgtgtt tttatgatgt
     7261 ttgctgtaat tatctgattt tgttttcttt gtatgtgatg aacttgtgat agagtggtta
     7321 tcgttaatct ggcaaagagg gaaaaacata ttatttaaac cattatagcg cataaaatat
     7381 ccttataatt aacaagaaag aaaaggcata ttctctgcat tatcattttc tacgactgtc
     7441 aaaaatcgct cattttttaa tgagtttatt tgtttaatat tatgggaaaa ggtgatgcat
     7501 gttggagagg aagagtattc cccggtcagg cgaccgggga aggggagaga ttaatgtgcg
     7561 cggccttgct caacgccaaa gccggtctgg gagcggataa actgcgcgcg gaacagttca
     7621 cgctcgcgcg ccccttccgc tgagttatcg gttgccgaga agaaccagat gccgaggaat
     7681 gccacgctga tagagaacag cgccgggtat tcatacggga agatggcttt ttcgtgaccg
     7741 aggatctgta cccaaatagt cgggccgagg atcatcagca ccactgcggt aatcagcctc
     7801 agccagccgc ccagcatcgc gccacgggta gtcagtttcg accagtacat ggaaagcaga
     7861 atgatcggga agttacagct cgccgcgatg gcaaacgcaa ggcctaccat aaaggcgatg
     7921 ttctgattct caaacagcac gccgaggata atcgcaatca cgccgaggat cagtacggtg
     7981 atttttgata cccgcagctc ttcacgttcg gtcgcgcctt ttttgaagac gttagcgtac
     8041 aagtcatgcg aaaccgccga tgcgcccgcc agcgtcagac ccgcaaccac cgcgaggata
     8101 gtggcgaaag caaccgctga aataaaaccg aggaataggt tgccgcccac tgcattcgcc
     8161 aggtgaacgg ccgccatgtt gttaccacca atcagatggc ccgccgcgtc tttatattcc
     8221 ggattcgcac caacaagcat gatcgcgccg aagccgataa taaaggtcag aatatagaag
     8281 tagcccataa atccggtggc gtagaacacg ctcttacgtg cttcgcgggc atcgctgact
     8341 gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac caaacatcag tcccagcccc
     8401 agagagagcg cggagatcgg atctttcacc agtccgcccg gtttcatgat gtcgacacct
     8461 ttcgggtgta ccgccatcgc ttcgctgaac agattgttga agctaaagcc gacgtgtttc
     8521 atcaccataa aggccataaa gctggcaccg aacagcaaca gcacagcttt gataatttgt
     8581 acccaggtgg tcgccagcat gccgccgaac aggacgtaca tcatcatcag cacgccgacc
     8641 agcatcaccg caatgtgata gttaaggcca aacagcagct cgatcagttt acctgcgcct
     8701 accatctgag cgataaggta aagcgccacc accaccagag aaccacaggc cgaaagaata
     8761 cgaatcggcc cttgtttcag acgataagag gccacatcgg cagaggtgta gcgtcccagg
     8821 ttacgcagac gttcggtaat cagaaacaga atgattggcc agcccaccag gaagcccaac
     8881 gagtagatca gcccgtcata gccggaggta aacaccagcg cggagatccc caagaatgag
     8941 gcggcggaca tatagtcccc ggcaatcgcc agcccgttct ggaagccagt gatattgccg
     9001 cctgcggtgt agtagtcgct acgagaacgt acgcgttttg atgcccagta ggtaatgccg
     9061 agcgtaaaca cgacgaaaat caggaacata ataatcgcct gccagttcgt tggctggcgc
     9121 tctacggccc cgctaatagc atccgcggcg ttagctgcga aagggagtgt ggcggcaagc
     9181 gccgtcagaa ctcttttcat gatgcttgta cctcatgcag gacttcatta ttaagacggt
     9241 cgaattcgcc gttcgcccgc cagatgtaga taccggtgag aacaaaggag atcacaatca
     9301 ctccaacacc aattggaata ccgcgtgtga cgctggtgtt cggattcagc ggcgtgccca
     9361 gccagccggg cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc agcataataa
     9421 tcgacaggat ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa tgcgcattgt
     9481 cttctatccg ctgataaatc gtaccattca tcacagattc tccagaggta atgtagggat
     9541 tgttttaatt cccgctccct tatgggagaa ggttaacgct cgggtaaccc ttgccgaatg
     9601 taggccggat aaggcgttta cgccgcatcc ggcaatcaat gcctgatgcg acgctgtcgc
     9661 gtcttatcag gcctacaaac cgttaccgat tcgcatccgg caattgtggg ttacgatggc
     9721 atcgcgatag cctgcttctc ttcaagcagc ttctcgacca cgccaggatc ggcaagcgtc
     9781 gaggtatcgc ccaggttgct ggtatcgccc gccgcaattt tgcgcagaat acggcgcata
     9841 attttgccgg agcgggtttt aggcagggag tcggtccagt gcagcacgtc tggcgtcgcc
     9901 agcgggccaa tctctttacg cacccagttg cggacttctg cgtacagttc tggtgacggt
     9961 tcctccccgt gattaagcgt gacataggcg tagatcgcct gacctttaat attgtgcgga
    10021 atacctacta cggcggcttc ggcaatcttc ggatgcgcca ccagcgccga ctctatctct
    10081 gccgtcccca gacggtgacc ggagacgttc agcacgtcgt ccacacgccc ggtgatccag
    10141 taatagccat cttcatcgcg acgcgcgccg tcgccgctga aatacatatt tttgaaggtg
    10201 gaaaaatagg tctgctcaaa acgttcgtga tcgccaaaca gcgtacgcgc ctgaccaggc
    10261 caggagtcgg tgatcaccag gctaccttcg gtagcccctt ccagcgggtt accttcgtta
    10321 tcgaccagcg ccggttgcac gccgaagaac ggacgtgttg ccgaaccggc tttcagctcg
    10381 gtagcgccag gcagcggggt gatcatgaaa ccgccggttt cggtctgcca ccaggtatcg
    10441 accaccggac atttctcgtt gccgattttt ttccagtacc actcccacgc ttccgggtta
    10501 attggctcgc ccacggaacc gagaatgcgt agcgacgaac ggtcggtgcc ttcaatcgct
    10561 ttatcgcctt ccgccatcag cgcgcggatc gccgtgggtg cggtatagag aatattgacc
    10621 tgatgcttgt ccaccacctg cgccatacgg gcaggcgtcg gccagttcgg tacgccttca
    10681 aacatcagcg tggtcgcgcc gcaggccagc gggccgtaca gcaaataact gtgtccggta
    10741 acccagccca catcggcggt gcaccagtag atatcacccg gatgataatc aaagacatat
    10801 ttaaaggtca gcgccgcgta caccagataa ccgccggtag tgtgcagtac gccttttggt
    10861 tttccggtag aaccggaggt atagagaata aacagcggat cttcggcgtt catctcttcc
    10921 gcctggtgct gatcgctggc ttgctcaacc aggtcgtgcc accacaggtc gcgcccttcc
    10981 tgccagtcga tgtttccgcc agtacgcttc agtaccacca catgctctac gctggtgacg
    11041 ttcgggtttt ttagtgcgtc atcaacgttt ttcttcagcg gaatactacg cccggcgcgc
    11101 acgccttcgt cggaagtgat caccagtcgt gagttggaat caataatgcg cccggcaacg
    11161 gctttcggcg agaagccgcc gaaaatcacc gaatgcaccg cgccaatgcg ggcgcaggcc
    11221 agcatcgcaa ccgcggcttc cggcaccatc ggcatataaa tcgccaccac atcacctttt
    11281 ttaatgccca gctcgagcag ggtattggcg aagcggcaga cgtcgcggtg cagctcttta
    11341 tagctgatat gtttgctctg gctggcgtcg tcgccttccc agatgatggc ggtgcgatcg
    11401 ccgttttctt gcagatggcg gtcaaggcag tttgccgcca gattcagcgt gccgtcctcg
    11461 taccatttaa tggacacatt accgggggca aaggaggtat ttttcacctt ctggtacggt
    11521 ttgatccagt cgagaatttt tccctgttcg ccccagaagg tatcaggtac gttaatagat
    11581 tgttgataca tcgcctcgta ctgctgaggg tttatcaggc aacggtctgt gatgttggca
    11641 ggaatggtgt gtttgtgaat ttggctcatg cttttgttct ccttgtagga tgttaataat
    11701 atgtggcata agcgttaaat gtaggggtat tggcagtttt gtttagtatt tgggcgacag
    11761 atcacgcaaa agtagaatgg tgcaaataaa cggcagggta atttttgaag gtcaccaaca
    11821 aaagttgatt aattctttga ggaacatgca gttatgcatg ctgttgaaaa agaggaagat
    11881 actaactaac tctaaagtgg tattttacat gcacttacaa ttgattaaag acaacattca
    11941 cagtgtggtt atttgttaca cataggggtg agcaatgtca tgacagtgta ggtgcggtta
    12001 ctgtcgtgaa aaacaataaa aaccgccatt gcaacaatgg cgcaattcgg atgaagcccc
    12061 tatgacaagg ataaaaataa acgcacgccg tatcttcagc ttattgattc cttttttctt
    12121 tttcacttct gttcacgctg aacaaacggc agctcccgca aaacctgtaa ctgtggaagc
    12181 gaagaatgaa acctttgccc cgcagcatca cgatcaatat ctctcctgga aagccacctc
    12241 ggaacagtca gagcgtgttg acgccctgga ggaagatcca cggctggtga tcctgtgggc
    12301 ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga catgcctttg ctgtgaccga
    12361 tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct gaagatggtc cgctaccgat
    12421 ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg atccagaaag acggcgaaga
    12481 tggctacttc cacggtaaat gggcgcgcgg cggcccggaa atcgtcaaca acttaggttg
    12541 tgccgactgc cataacaccg cctcaccaga gttcgccaaa ggcaaaccgg agttaaccct
    12601 ttcccgtccg tatgcggctc gcgcgatgga agccattggt aaaccttttg agaaagccgg
    12661 acgtttcgac cagcaatcga tggtttgcgg tcagtgccat gtggagtatt acttcgacgg
    12721 caaaaacaaa gcggttaaat tcccgtggga tgacggcatg aaagtcgaaa atatggagca
    12781 gtattacgac aaaattgcct tctctgactg gactaactcc ctgtcgaaaa cgccaatgct
    12841 gaaagcgcag cacccggaat atgaaacctg gacagcgggc attcacggta aaaacaacgt
    12901 gacctgtatc gactgccata tgccaaaagt gcagaacgcc gaaggcaaac tctacaccga
    12961 ccataaaatt ggtaatccgt ttgataactt cgcccagact tgtgcgaact gccataccca
    13021 ggataaagct gccttgcaaa aagtggtcgc ggaacgtaag caatcgatta acgacctgaa
    13081 aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa gcgaaagcgg cgctggatgc
    13141 aggcgcgacg gaagccgaaa tgaagccgat tcaggacgat atccgtcatg cccagtggcg
    13201 ttgggatctg gcgatcgctt cccacggtat tcatatgcac gcaccggaag aaggtctacg
    13261 gatgctcggt acggcgatgg ataaagcggc ggatgcgcgc accaaactgg cgcgcctgct
    13321 ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat atctcgacca aagagaaagc
    13381 ccagcaggcc attggcctga acatggaaca aatcaaggcc gagaagcagg acttcatcaa
    13441 aacggtgatc ccgcagtggg aagagcaggc acgtaaaaac ggtctgttaa gccaataacc
    13501 ccgttccgcc tcgcaagggg cggaaaacac aatggagtga atatgagcgt attacgttcg
    13561 ttattaactg ctggggtgct ggcgtcgggc ctgttgtgga gcctgaacgg aattaccgct
    13621 acccctgcgg tgcaggcatc tggtgatcgc tacgaagtta cccagcagcg taacccggat
    13681 gccgcctgtc tggactgtca taaaccagat accgaaggta tgcatggcaa acatgcttcc
    13741 gttatcaacc cgaataacaa attgccggtc acctgcacca actgtcacgg ccagccatca
    13801 ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta acgagccgat gtacaaggtc
    13861 ggagagcaga acagcgtctg tatgtcctgt catctgccgg aacagttgca aaaagcgttc
    13921 tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca gctgccattc cctgcatcca
    13981 cagcaagata cgatgcagac gttaagtgac aaaggacgga ttaagatttg cgtcgattgc
    14041 cacagcgatc agcgcaccaa tccgaacttt aacccggcgt ctgttccgtt gcttaaggag
    14101 cagccatgac ctggtctcgt cgccagtttc tcaccggcgt cggcgtgctg gcagccgtca
    14161 gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat caatggggtg cgttacggca
    14221 tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg tatggatgct tgtcgggaag
    14281 tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat tcgtagcgag ccgcagggcg
    14341 aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg ccagcactgc gagcatgcgc
    14401 cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga tgccgctagc gggattgtcg
    14461 atgttaatcc tgatctctgc gtcggttgtc agtactgcat cgccgcctgt ccgtaccgcg
    14521 tgcgctttat ccatccggtc acgaagacgg cggacaaatg cgatttctgt cgtaagacca
    14581 atttgcaggc cggtaagctt cccgcgtgcg ttgaagcttg cccgacgaag gcgctgacgt
    14641 ttggcaatct ggacgatcct aacagtgaga tttcgcaact gctgcgccag aagcctactt
    14701 accgctacaa gctggcgctg ggaaccaaac cgaagctgta ccgcgtaccg tttaaatacg
    14761 gggaggtgag ccaatgacgc agacttccgc atttcatttt gaatcgctgg tgtgggactg
    14821 gccgattgcc atctacctgt ttttgattgg tatttctgcc ggtctggtga cgctggccgt
    14881 gctgttacgt cgcttctacc cgcaggcggg cggtgcagac agtacgttgc tgcgcaccac
    14941 gctgattgtc gggccgggcg cggtgatcct cggtctgttg atcctcgtct tccacctgac
    15001 aagaccgtgg accttctgga agctgatgtt ccactacagt tttacctcgg tgatgtcgat
    15061 gggggtgatg ctgtttcagc tctacatggt ggtgctggtg ctgtggctgg cgaaaatctt
    15121 tgaatatgat ttgcttgccc tgcaacaacg ctggttgccg aagctgggga tcgtgcaaaa
    15181 ggttctgagc cttctgacgc ccgttcatcg cggactggaa acgctgatgc tggtgttggc
    15241 ggtgctgctg ggggcttata ccggctttct gctgtcggcg ctgaaatcgt atccgttcct
    15301 caataacccg agcctgccgg tgctgctcct cttctccggc atctcgtccg gtgcggcggt
    15361 ggcgctgatc gccatggcga tacgccaacg cagtaacccg cattccacgg aagcgcactt
    15421 tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc ttcctgctgg tggcgttttt
    15481 tgtcggtctg gcgctgggcg atgacggtaa agttcgtgcg ctggtggcgg cattaggtgg
    15541 cggtttctgg acgtggtggt tctggcttgg tgtcgccggg ctggggctga ttgtgccaat
    15601 gttgctcaaa ccgtgggtca atcgcagttc cggcattcct gccgtgctgg cggtgtgtgg
    15661 ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt ctctacgccg gacagttgac
    15721 ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt ccttcctgaa gtcggttttc
    15781 tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc gttgacggcc tttgcgggag
    15841 tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat cggcattctg gcgcagttcg
    15901 cgctcctgct gctcgccttt ggcgtactga cgtattgttt tctcatcagc gatttctcgg
    15961 tcatttatgt cgcccaacat agctacagcc tgctgtcgtg ggaacttaaa ctggcggcgg
    16021 tgtggggcgg tcatgaaggt tcgctgctgc tttgggtgct gctgctttcc gcctggagcg
    16081 cgctgtttgc ctggcattat cggcagcaaa gcgatccgct atttccgctg acgctagccg
    16141 ttttatctct catgctcgcc gcactgctac tgtttgtggt gctgtggtcc gatcccttcg
    16201 tgcggatatt tccaccagca atcgaaggcc gcgatctcaa tccgatgctg caacatcccg
    16261 gtcttatctt tcatccaccg ctgctttacc ttggctatgg cggtttgatg gtagcggcga
    16321 gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc ggccagcgcc cgaatttgct
    16381 ggcgctgggc gttacctggc tggagtgcat taacggcggg gatcatcctc ggttcctggt
    16441 gggcctactg cgaactcggc tggggcggct ggtggttctg ggatccggtg gaaaacgcct
    16501 ctttattacc ctggctttct gccactgcgc tgctgcacag tttgtccctg acacgccagc
    16561 gggggatttt ccgccactgg tcgctgttgc tggcgatagt tactctgatg ctgtcgctgc
    16621 tgggcacctt aattgtccgt tctggcattc tggtttcggt tcatgcgttc gcactggata
    16681 acgtccgcgc cgtgccgttg ttcagcctgt ttgcactgat tagccttgcg tctttggctc
    16741 tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg tttttcgggg ttatcgcggg
    16801 aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt gctactgatc gtgctggtgg
    16861 gaacgcttta tccgatgatt tacggcctgc tgggctgggg acgcctctcc gttggcgcgc
    16921 cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat gctggtggtg attgtgctgg
    16981 cggcgtttgt ctctggcaaa cgcgtgcagc ttccggcgct ggtagctcat gctggcgtgc
    17041 tgttatttgc cgcggggatc gtggtttcca gtgtcagtcg tcaggagatc agcctgaatt
    17101 tacagccggg tcagcaggtg acgctggcag gatacacctt ccgttttgag cgactcgatc
    17161 tgcaagccag aggcaattac accagcgaaa aagcgatagt ggcgctgttt gaccatcagc
    17221 aacgcattgg tgaattaacg ccggagcggc gtttttatga agcacgccgt cagcaaatga
    17281 tggaaccgtc aattcgctgg aacggcatcc atgactggta tgcggtcatg ggtgaaaaaa
    17341 ccagagcgga tcgttacgct tttcgtttgt atgtacaaag cggtgtgcgc tggatctggg
    17401 ggggaggatt gttgatgatt gcgggcgcat tgttaagcgg atggcggggg aagaagcgcg
    17461 atgaataaag ggcttctcac gctactgctg ttatttacct gttttgcgcg cgctcaggtt
    17521 gtagacacct ggaaattcgc caatccgcaa caacagcaac aggcgttaaa tattgccagc
    17581 cagttacgtt gtccgcagtg ccagaatcaa aacttactcg aatccaatgc gccggtggcg
    17641 gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg ggaagaacga agtcgaaatc
    17701 attggctgga tgaccgaacg ctacggagat tttgttcgct ataacccacc gttaacgggt
    17761 cagacgctgg tgttgtgggc gctgccagtg gtgttgttat tgctgatggc actgatcctc
    17821 tggcgagtga gggctaagcg atgaaacagc ctcaaatacc ggtgaaaatg ctgacaacac
    17881 tcacgatatt gatggtattc ctctgtgtcg gcagttatct gttaagccca aaatggcagg
    17941 cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca tcagtttgcc agccagcaaa
    18001 ccccggaagc gcagcttcag gcattgcagg ataaaatccg cgctaatcca caaaacagcg
    18061 aacaatgggc gttactgggc gagtactatc tgtggcaaaa cgattacagc aattcgctgc
    18121 tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc tgaactgtat gcggcgctgg
    18181 cgacggtgct ttattaccag gccagccagc atatgaccgc ccagactcgc gcaatgatcg
    18241 acaaagccct cgcgctggac tctaacgaaa tcaccgctct gatgctgctg gcttccgatg
    18301 cgtttatgca ggcgaattac gcgcaagcca tcgaactatg gcaaaaagtg atggatctca
    18361 actcaccccg gatcaaccgg acacagctgg tcgagtcgat taatatggca aaactgttgc
    18421 agcggagatc ggattaatca tctggtgatg aagattatgt cattgagctg cataaaaaat
    18481 aatcgaatga acatatgcca aaaataatca ccaatcagta ttattgcaga ttaaacaaat
    18541 aaaaatcttt ccataacaaa tggttattca ttaatcctgc cataaactcg cgttattatg
    18601 cattaatgca gcgaaaagct ctgttgttaa agggttgcgc aacataccgc gcaaatgata
    18661 ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac acaattcatg ccctggcagt
    18721 atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc attgaggaag ttattctgat
    18781 gaaaaatata aaattcagcc tggcctggca gattctgttt gctatggtgc tgggcattct
    18841 cctgggaagc tacctgcact accatagcga cagccgcgac tggctggtcg tcaatttgct
    18901 ctctccggcg ggtgatatct tcatccatct gatcaaaatg attgttgtgc cgattgtgat
    18961 ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc aaacagctcg ggcgtattgg
    19021 cgcgaaaacc attatttact tcgaggtgat caccaccgtc gccatcattt tggggatcac
    19081 tctggcgaac gtcttccagc ccggtgccgg ggtggatatg tcgcagctgg cgaccgtcga
    19141 tatctcgaaa tatcagagca ctacggaagc ggtacaaagc agttcccacg gcattatggg
    19201 cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg atggcgaaag gcgaaatgct
    19261 gccgatcatc tttttctcgg tgctgtttgg tctggggctt tcttccctgc ccgcgacgca
    19321 tcgtgaaccg ctggtgaccg tgttccgctc catctctgaa accatgttta aagtgactca
    19381 catggtgatg cgttatgcac cggtgggtgt gtttgcgctg attgcggtga cggtggctaa
    19441 ctttggtttc tcgtctctgt ggccactggc gaaactggtg ctgctggtgc atttcgccat
    19501 tctgttcttc gcgctggtag tgctgggaat tgtggcacgt ctgtgcgggt taagcgtctg
    19561 gagcctgatt cgtattctga aagatgagct gattctggcg tactccactg ccagctctga
    19621 aagcgtactg ccgcgaatta ttgagaagat ggaagcctac ggagcaccgg cgtcgatcac
    19681 cagcttcgtg gtgccgaccg gttactcttt taaccttgat ggttcgacgc tgtatcaaag
    19741 tattgccgct attttcatcg ctcagctgta tggcattgac ctgtccatct ggcaggaaat
    19801 cattctggtg ctgacgctga tggtgacctc gaaagggatt gctggcgtgg ctggcgtgtc
    19861 gtttgtggtg ttgctggcaa cgctgggtag cgtaggtatt ccgctggaag gtctggcgtt
    19921 tattgctggt gttgaccgta tcctcgacat ggcgcgtact gcgctgaacg tggtgggtaa
    19981 tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa tttgaccgca aaaaggcgct
    20041 ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact gcggatcaat aattgaagat
    20101 tgccggggat atcccccggc aatgtgtgga tgcctgatgc gacgcttgcc gcgtcttatc
    20161 aggcctacct taaccgttgg ttaatttttc aaactcttca cacccggtat caaacccttc
    20221 catacagctc aggttcaacc agtgcagcgc cttttgtttg ttcttctcga taaaaccttc
    20281 ctcaccgttt aagaacatca ttcccgccca gtactcggaa taaccggttc gggaaattgc
    20341 agagctgcgt ttgaaatacc aggttgcctt gtcatcgtcg gccttaatgc ccacgccgtt
    20401 ggcgtaaatc aaaccaagca gcatttgggc atcgacggcg gagtcattct ccagatcttc
    20461 cgaggcgttt tctaacagcg aaatcgcctt tggataatcc ggtttacccg cttgagtatt
    20521 taccagaata tgcgccagcg ttacttcacc ttctttactg cccgcttgcg ccgctttctc
    20581 tgcaagaact tttgcttgtg gatagtccag actgaccgga ttggtgattt tgatctgcgc
    20641 caacagcgcg caggcatcag catcaccgct gtcggcggct ttctgtgccc aatattcggc
    20701 tttgctcaaa tcgccggagc taaaccagct gtcggcaaga aaatattgtg cgcgttggtc
    20761 cccggcctct gctgccttta aatactggct gccaggctcg gagtcgttcg cgtgggcaaa
    20821 gaatgtcaaa aacaacatca atgcgataat ttttttcatt ttgataatac gattgggtag
    20881 ggagtaacca gtataaagag ttcgaatgga taaaaaaaca gcctccgaaa ggaggctgta
    20941 gaaaggactg tattacgcca gtgccgcttc gcgcaggcga gttttcaact tgttgtactc
    21001 gtcaatcagg tactgctcgg cggcgcgctg atcggagatc ggctcgacgc gcacggcgca
    21061 gtatttgtat tccggcgttt tcgtaatcgg gcttaagttt tcggtaacca gctcgttaca
    21121 ggcaccaatc caccactggt aggtcatgta aatcgcccct ttgttcggac gatcgctgac
    21181 ctgcgcacgg gtgataattt tgcctttacg cgagtgcact caaaccaatg cctcatcttc
    21241 aatacccaga cgtttggcgt cttcggtatt gatttgtgcg tagccaggtt catcagccag
    21301 cgccgccagt gccgcacagt taccggtcat cgaacggcaa gagtagtgac caacttcacg
    21361 caccgttgac agtaccatcg ggtactcgtc ggtgagtttg tcgattggcg ctacccagtc
    21421 gcaggtgaag aactgcgcca gaccgttcgg ggtatcaaac ttctctttaa acagataaga
    21481 agtcccctga tcggcatctg aagtatcgcg gcaaggccac tgaatgaagc ccagttcgcc
    21541 cattttctcg taagtcgcac cgtagaaatc cgggcacaga tgacgcaact catcccagat
    21601 ctcctgggtg ttgttgtagt gcatcggata acccatacgg gtggcgattt cactgatgat
    21661 ttgccagtcc gttttcagat cccatttcgg ttcaactgct ttgaagaaac gctggaagcc
    21721 acggtcagcc gcagtaaaca cgccttcatg ctcgccccac gacgttgacg gcaaaatgac
    21781 atccgccgcc gacgcggttt tggtcataaa gatgtcctga acgatgacca gttccagatc
    21841 ttcaaaggct ttacgtactg ccgacagctc cgcatcagtt tgcagcggat cttcgcccat
    21901 aatgtacgcg gcacgcactt cgccatgcgc tgcgcggtgc ggcagctcgc tgatgcgata
    21961 gccggtatga gcaggcaggc tttccacgcc ccaggctttg gcgaatttct cgcggttagc
    22021 cggatctttc acgtactggt atcccggata cgtatccggc agcgcgccca tatcgcaggc
    22081 accctgcacg ttgttctgac cacgaaccgg gtttacaccc gcatgcggct taccgaggtt
    22141 accggtcagc atcgcgaggc tggtcagaga acgcacggtt tccacgccct ggtagaactg
    22201 ggttacaccc atgccccaca ggatggcggc gcttttcgcc tgggcataca tccgtgccgc
    22261 ctgacgaatc tcactggcgc tgacaccggt gatatcttca accgactccg gcgtgtagcc
    22321 ttcaacgatt ttacgatact cttcaaagcc ttctgtacgg gaagcgacga acgctttgtc
    22381 gtacagattt tcttcaataa tgacatggcc catcgcattc aacagcgcga tgttcgagcc
    22441 gtttttcagt gcaatgtgca tgtcagcaat gcgcgcggtt tcaattttgc gcggatcgca
    22501 gacgataatt ttcgccccgt tacgtttagc gttaattacg tgattcgcca cgattgggtg
    22561 ggaatccgcc gggttgtacc cgaaaacgaa cactaaatcg gtattatcaa tttcgttaat
    22621 agcattgctc attgcgccat taccgaccga ttggtgcaga cctgcaaccg atgggccgtg
    22681 tcagacacga gcgcagcagt caacgttatt ggtaccaata acggcgcgcg caaatttttg
    22741 cattacatag ttggtttcgt tacccgtacc acgcgaggag ccggtcgtct ggatggcatc
    22801 cggaccgtac ttctctttga tggcgctcag gcgctcggca acgtaattca gtgcctcatc
    22861 tcaggaaaca ggttcgagtt tgccgccacg ctggcgacgg atcatggggg ttttcaggcg
    22921 cggggtcagg atctgggtat cgttaatgaa atcccagcca taataaccct tcagacacag
    22981 ggtaccctgg ttggttttcc cctgcgctgc ctccgcccgg acgattttgc cgttatcgac
    23041 gaccaggttg attttgcaac ctgatgcgca ataggggcaa accgtgacga cttttttcat
    23101 cggtctcgct ccagttaatc aaatcacgca tacgcgctct cgactacagt atgcatcttt
    23161 tatgccacat tttatttggg gtcattccct gatattacgg gcactattta ttcaaaactc
    23221 tgacgaaaaa caggctgtcg tcagttttga cgtgacgaaa cgaaataccg cgtgacagcc
    23281 atcacgcggc agacatttta ttttttctcg acgacgggag ccgcctgata cccgccgccc
    23341 agcgatttca tcaactgaat gctttggatc acccggcggc tgtccagcat cagtaatgac
    23401 atctcttcgg caagtactgg caaccgggct tcagtggcct gtaagcggct ggttaagccg
    23461 cgctgatagg cggcctcggc agcgcgctgg gtgaagcgcg ttgcttccac gtgttcagcc
    23521 tgcatttctc gctcgtcgtt gagcgtttgc agacgcgtgc cgttgacggc aacgtcacgc
    23581 accgcgttca gtactgactg gttgtaacgt tcaatcatca tgttgctggc ggcgcgcgtg
    23641 ccttcgagat tggcattcaa ccgtccaccg tcaaacagcg gcaatttcag acccgggatg
    23701 aagttgaact ggcgactggt ttttttgaat aaggtatcta ggtggatggc atcaaggccg
    23761 aaaaacgctt tgatatcaaa gctcggatag aacaacgccc gcgcggaatc cacctgatct
    23821 aatgacgcct gaacatacca gcgcatggct tgcagatccg ggcgtctggc gagcaactca
    23881 taagagagtg tcgccggaat gccggtctgg actcgtggta atgccacagg tttgatctcc
    23941 ggcatatcgc tggctcccgc gccaatcaat gcgcgcagag attctctcgt ttcggtgatt
    24001 tgccctttga cggcagcaat ttgtttatcg accgccagaa tctgtgcccg cgcgccgtgg
    24061 aaaggcactt gtgcttccag accgtgcgcc actttacttt ggtgcgcttt caccgcgtaa
    24121 tcaatcacat cgcgagtttg ttctaacaga tcgagcatct gatagctggc ctgcatactg
    24181 taataaagct gcgctacgcc cgtggtcagc gatagctcta ctgctgcggt ttctgccagc
    24241 gcggcattat gcgcgccaat ggcggcggca accgctgagc gatgcacacc ccacaaatca
    24301 agatccagtc cggcaaacaa acctactgtg gcttccgtat agtacggccc gtccatcccc
    24361 agtgcgggcg catccatcgc ataagggctt aaaaagccgt tcgccgagac acgttggcgg
    24421 ttaagcatcc ctaacgctgc cacctgcaac tgtgaaccgg catctaacaa atcggcctgc
    24481 gattgcgctt tttcttcccg cagtttcgct tcggcgaggg tgtgtgaacc acttagcgtc
    24541 cgttggatca gggcatccaa ctgcgggtca ttgagttgtt tccaccactg cgcctgcggc
    24601 cagccggagc tggcaagatg aatatcatcg gccagtttga tttgttccgg tttgagctgt
    24661 tgatgaggcg cagaatcctt acgtaccagg gcacagccgg aaatcagcgt cgtgctgccg
    24721 agaatgctgc acaacagcag acgtgaaagt tgacgattga tcattgcgtg gctccttgtg
    24781 cctgttccgt ggcgggcgtt aatgccgggg ctgtccagtc tggcaagcgg gcgaaaagct
    24841 ggcagacgtg ttgctcctgt tttaataagg tgggcaggat ggcctgcgag gcgggtgtct
    24901 cttcaagcgt tatttgagga gaacggctga gtgcggttgc cagaccggca gcgtatttct
    24961 ccagggcgtc ggcaaactga cctgctctgt ccggctgtag tgcgttatcc agcgcctgcg
    25021 ccgaattcca ggtggcatcc caggcgtgaa ggatatcgcg gccctgacga ataaccgttt
    25081 gcgaacgttc aatcagtaag gcgcgttctt cgctgtccag ttgacgctcc agcgccacgc
    25141 gttggcacat ctcttcacag gcattaaacg ccgcatgcag accgatacga atttgcagat
    25201 aagtgcgcag agccgtgact tcctgctggc gtgggatccg cattacttta cttaacatac
    25261 ccagcgcgcc agccagtttt tgcggcagcg tgcgcgcttc actttcaggc cagacaaagg
    25321 tgtaaatcat cgcggacacc acggtaccaa tgatgatacc cagggcgcga tcgcgaattt
    25381 ccaccaggtc gtacactggg ccaaaaacgt tttcgagcgt ggcgagcgcg aaggtgacca
    25441 ccatctgtgt gccgatatta gaagagcgtt cagagctggt ggcaatctat gcgcccaaca
    25501 ggaaaatcgg tgccagcaca aacagcaatt cgacaatatt gtccagccag ggcatgacca
    25561 gtagcgtgaa taacagcgcc agaatcgcgc cgcaaaaggc cccgccaaaa cgcagcacca
    25621 tcttctggta cgacgaaccg acatttggat tagcgacgat cacgcaggtc agcatacagg
    25681 tgtgaatgcc ttcccagtcc acgccgctgt aaaaggtgta acagatcaaa caggcgagca
    25741 atgtttttac cgcgtagcgt atatagtctg gattggtaaa agcatcggcg accattgatg
    25801 gcggtttggc ggcgggcgtt ggcggcgtat tcgggttcat ctggcccagt tgtaacaacg
    25861 tctggcagat attctccagg ttacattccc gtgccgccac cgcttcactt tcactgatcc
    25921 gccagtcgct ttgccagcac tgaccttcag caacggcatg ctgcagcttg ttgatttctg
    25981 aggctaattt ttgccgaaat tcaataattg cctgagaatc agcaaaagag gtgggatcgt
    26041 agcgattcag cgtcgagtaa atgtaggtta ccgttgccac gcagctttgc caccatgcgc
    26101 tttgagttcg ccagttggca tcgtccgcga gacaaaagac attcagtttt tgcagcgcca
    26161 gcgcctctct ttcaatccgc gtttcgggta gcggtgcgag gctgtccgtc aggtggctaa
    26221 tggcatcatc aagccgatca ttaagcgctt gatgcatttg cgtaatggca cgattgggaa
    26281 accacagcac gccgattaac gtcatcagca aggttggata gaggccaaca acgatacacc
    26341 acagcgttaa gcgcacgact acttccggat agtcgagcat ggcggggaag gtttgcccgt
    26401 aaatggcgac aatggcgaca gcgaaaaaga ccagtcccag ccgatgggtg cgcatcaaaa
    26461 acatgcagcc catcaggatc ggcccggcga tgatcaatcg gatcaacggt tcgccgtatg
    26521 accatttgta gatcaaaaac aggctgccga tctccagcac cgtggcaacc acaaacaaga
    26581 tcgccacaaa tttggtgtaa aacgcgttcg actgaatacc gtaaaacagc actgccagcg
    26641 ataacgccac aaaagggatc tcaaaagtca tcgagatcag aatcaccagc aggcagccta
    26701 cccagagttg cacgatctgc ggcacgcggc cgggtcgccg ctcgcttaac tcttcatgaa
    26761 agaacgccag cagcctgacc accggtaatg gcagggagtt gagcgcgctc attattgcgg
    26821 ctcaagatta gcgactgccg aagcgccgat gcggaacatt tccgggtcag gtttatcgac
    26881 catgattttg accggaaaac gctgggcaac gcggacccag ttaatagaac gagacacttt
    26941 tggcaggccg cccagcacca ggccgccgtc atccggtagc acgccgtagc caatcgaatc
    27001 cactttaccc tcgaaggttt tgccgctgtc actcatcagg cgaatcgttg cgggtgtacc
    27061 tgagcgaata tttttcagat cggtttcgcg gaagttggcg atcacgtacc agtgacgagt
    27121 gtcgatgagg gtaaaaatag ggcgcatggc agaagcaaat tgcccgacgg aggttttgag
    27181 ggaaatgatc cggccatcaa atggcgcgcg aacggtcgtc atttccagat gtagtttggt
    27241 cagggcaata tccgcttcga gcgccgcacg ttgcgccacc agcgcatcga cgccgctgac
    27301 ggcgctggcg gctgactgcg cctgtaacaa tacggcatta agatccgctt ctgcggcgcg
    27361 ctgcgccgtt cttgcacggt caacgtcttc tgctgagaca aaaccctctt tcagtaatgg
    27421 ctcggtgcgg cgtaatgtat ctgtggcctg tttcgcggcg gcacgggctt tttctaccgt
    27481 ggcattaacc gagtcgtcac caaactgttg ggcgtcaacg ctacgctggg tgagcataat
    27541 ttgcttatcc agcgccgcga gggaggcttc ggctttcgcc aggttggctt cgtacgggcg
    27601 cgggtcgatg cggaacagca aatcgccctg tttcaccgcc tggttgtcgg tgaccgccag
    27661 ttctacaatg cgaccgctga cttccggcac cacatcaatg gtatctgctg acgcgtaagc
    27721 gtcattagtt gatggcgcac tgtctacgcg ccagataacg aaaacaaggg caaccagcgc
    27781 caacgcaacc actaacagag cagggaattt actgcgaggt gcttttttcg gcgtactttc
    27841 catgagcgcg tctcaaatag attagaaaaa tgccagccag aacagcatgg cgtagagagc
    27901 aaaaagggcg gtataaataa ttccgacaaa tgccagattg atatttgccc gctgaataac
    27961 tcgcctcgtg atgagcgtca aaataaggct ggcaatggcg cagaaaaacc agctgggata
    28021 ataagcgccg atcaccggga ttgccggaga taatgagcat cccgaaacca tgaagacggg
    28081 aattatccag gctgtttttt tgaggtgtat cgccatgcga gagagaactg ttggcatatt
    28141 aaatacatcc tgtctgctgc gaacaatatc tgtagtgaat atcaatatcc attgaaggcg
    28201 tcagtatggc tattttctcg atgaataaaa atagctttat tcagccaccg cattcataac
    28261 ttatatattt tatttattaa ttggctgtat atatttttaa aataaatgct tttgggggct
    28321 tcagcgcccc caaaagcgag tgacattaat ttggggttac gatattcacc cacaaatcga
    28381 aattatccag acaggcgata atttcttcca gtttcgcgcc attgccaata attttggctc
    28441 tcttcgcttt caccagatcc gcc
//
LOCUS       DADMEQ010000045        27688 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.045, whole genome shotgun sequence.
ACCESSION   DADMEQ010000045 DADMEQ010000000
VERSION     DADMEQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27688
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.045"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..704)
                     /locus_tag="JNZ05_001955"
     CDS             complement(<1..704)
                     /locus_tag="JNZ05_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565197.1"
                     /translation="MGPLPRKGNMKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRL
                     ITQRRKKHWDESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWS
                     SDDGFSVIDYHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGF
                     EDFFIAVDPKTDLSAVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLL
                     AMVDVLLCYLEKGAEYV"
     gene            complement(889..1203)
                     /gene="pspE"
                     /locus_tag="JNZ05_001956"
     CDS             complement(889..1203)
                     /gene="pspE"
                     /locus_tag="JNZ05_001956"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HAY8565198.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(1278..1499)
                     /gene="pspD"
                     /locus_tag="JNZ05_001957"
     CDS             complement(1278..1499)
                     /gene="pspD"
                     /locus_tag="JNZ05_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HAY8565199.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1508..1867)
                     /gene="pspC"
                     /locus_tag="JNZ05_001958"
     CDS             complement(1508..1867)
                     /gene="pspC"
                     /locus_tag="JNZ05_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HAY8565200.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(1867..2091)
                     /gene="pspB"
                     /locus_tag="JNZ05_001959"
     CDS             complement(1867..2091)
                     /gene="pspB"
                     /locus_tag="JNZ05_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HAY8565201.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2145..2813)
                     /gene="pspA"
                     /locus_tag="JNZ05_001960"
     CDS             complement(2145..2813)
                     /gene="pspA"
                     /locus_tag="JNZ05_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HAY8565202.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            2980..3957
                     /gene="pspF"
                     /locus_tag="JNZ05_001961"
     CDS             2980..3957
                     /gene="pspF"
                     /locus_tag="JNZ05_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HAY8565203.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLQDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4077..5342)
                     /gene="puuE"
                     /locus_tag="JNZ05_001962"
     CDS             complement(4077..5342)
                     /gene="puuE"
                     /locus_tag="JNZ05_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HAY8565204.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5380..6660)
                     /locus_tag="JNZ05_001963"
     CDS             complement(5380..6660)
                     /locus_tag="JNZ05_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY8565205.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(6662..8149)
                     /gene="puuC"
                     /locus_tag="JNZ05_001964"
     CDS             complement(6662..8149)
                     /gene="puuC"
                     /locus_tag="JNZ05_001964"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HAY8565206.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYAAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8299..8856)
                     /gene="puuR"
                     /locus_tag="JNZ05_001965"
     CDS             complement(8299..8856)
                     /gene="puuR"
                     /locus_tag="JNZ05_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HAY8565207.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(8883..9647)
                     /gene="puuD"
                     /locus_tag="JNZ05_001966"
     CDS             complement(8883..9647)
                     /gene="puuD"
                     /locus_tag="JNZ05_001966"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HAY8565208.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            9859..11277
                     /locus_tag="JNZ05_001967"
     CDS             9859..11277
                     /locus_tag="JNZ05_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HAY8565209.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIDQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11426..11590
                     /locus_tag="JNZ05_001968"
     CDS             11426..11590
                     /locus_tag="JNZ05_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565210.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            11580..12965
                     /gene="puuP"
                     /locus_tag="JNZ05_001969"
     CDS             11580..12965
                     /gene="puuP"
                     /locus_tag="JNZ05_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HAY8565211.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13099..13344
                     /locus_tag="JNZ05_001970"
     CDS             13099..13344
                     /locus_tag="JNZ05_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HAY8565212.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTHLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            13657..15300
                     /gene="sapA"
                     /locus_tag="JNZ05_001971"
     CDS             13657..15300
                     /gene="sapA"
                     /locus_tag="JNZ05_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HAY8565213.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15297..16262
                     /gene="sapB"
                     /locus_tag="JNZ05_001972"
     CDS             15297..16262
                     /gene="sapB"
                     /locus_tag="JNZ05_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HAY8565214.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16249..17139
                     /gene="sapC"
                     /locus_tag="JNZ05_001973"
     CDS             16249..17139
                     /gene="sapC"
                     /locus_tag="JNZ05_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HAY8565215.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17139..18131
                     /gene="sapD"
                     /locus_tag="JNZ05_001974"
     CDS             17139..18131
                     /gene="sapD"
                     /locus_tag="JNZ05_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HAY8565216.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18133..18939
                     /gene="sapF"
                     /locus_tag="JNZ05_001975"
     CDS             18133..18939
                     /gene="sapF"
                     /locus_tag="JNZ05_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HAY8565217.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19007..19360
                     /locus_tag="JNZ05_001976"
     CDS             19007..19360
                     /locus_tag="JNZ05_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HAY8565218.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            19728..20516
                     /gene="fabI"
                     /locus_tag="JNZ05_001977"
     CDS             19728..20516
                     /gene="fabI"
                     /locus_tag="JNZ05_001977"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAY8565219.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            20660..21787
                     /locus_tag="JNZ05_001978"
     CDS             20660..21787
                     /locus_tag="JNZ05_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HAY8565220.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPAIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            21855..23789
                     /locus_tag="JNZ05_001979"
     CDS             21855..23789
                     /locus_tag="JNZ05_001979"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HAY8565221.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(23867..23969)
                     /locus_tag="JNZ05_001980"
                     /pseudo
     CDS             complement(23867..23969)
                     /locus_tag="JNZ05_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24023..25997
                     /gene="pdeR"
                     /locus_tag="JNZ05_001981"
                     /pseudo
     CDS             24023..25997
                     /gene="pdeR"
                     /locus_tag="JNZ05_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26145..26318
                     /locus_tag="JNZ05_001982"
     CDS             26145..26318
                     /locus_tag="JNZ05_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565222.1"
                     /translation="MSEFDTQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26408..27157
                     /locus_tag="JNZ05_001983"
     CDS             26408..27157
                     /locus_tag="JNZ05_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY8565223.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27426..27644
                     /gene="osmB"
                     /locus_tag="JNZ05_001984"
     CDS             27426..27644
                     /gene="osmB"
                     /locus_tag="JNZ05_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HAY8565224.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         6437 a         7151 c         7117 g         6983 t
ORIGIN      
        1 acatactcag cacctttctc aaggtaacag agtaaaacat ccaccatcgc cagcaacact
       61 tcagggctac ggtaattcag gtcaatttga tcgtcactaa aggtggtcca taaatggcgc
      121 gtggaattat catccatttg aaacggcgtt aatagcggta acgcacgcgg acgggtgacg
      181 gcactgagat cggttttcgg atcaaccgca ataaagaaat cttcaaaacc cggaagctgt
      241 tgtaaatagt ttttaaacca ttcacttttt gcagacatat ggttgcagac aaaatcaaac
      301 attaaatgac tgcattcacc gagttgctga atatcctgcc actcccccgc ttcactggcg
      361 acctgatgat aatcaattac cgaaaatcca tcatcagatg accatgggta aaaaggtaat
      421 aaatgaacat gtgaaaaaat gctttgcagc cattgatggt agaactgatt aaatatgggt
      481 agtggtttta aatcattact gtgaaattga tcggcatagg taattaacac gacatcactt
      541 tcatcccagt gttttttacg tcgctgtgta attaatcgtt tcgcactctc aagacgcgtt
      601 acaagtttct gtaaatgagt tgcggtaaat gttccaccgt agatttcgtc caggtaatcc
      661 gtaattttct gtttcatgtt gcctttccgt ggtagcggtc ccatgaggcg agagactact
      721 ccgcagaaaa taaactgtca attgagcaca agggttttag tcacgcagga aatcattttt
      781 taccagggaa aaagcgtatt tgtgagctga cgcaaataat ctatagggat gtaaataagg
      841 tgtgacagaa aaaaaacggc gcataagcgc cgctcatggt gaattctttt aacctttgac
      901 cttcggcatt gcgatgtctt tcaggccacc ggcgttctca acgtgggtat atcccatctc
      961 gctaaggatc tcttttgcct gcccggactg gcgtccggca ttgcaataca ctttcacggt
     1021 gtcgttttta tccggaacgg cggtggcaat gcgctctttc acttccttca ggggaatatt
     1081 gatggccccc tgaacgtgct cttgctgata ctgctctgga acacgaacat cgatccagtg
     1141 ttcagcggcg aaaacgggca gtgaaaacac cagtgccaga gcaagtaagc cttttttaaa
     1201 catgtaagcg tccttctatg gatgaaaatt gccatccatt atacgcctga gatttaacaa
     1261 tttttgcgaa actcccctca ccttttataa cgctgtgcca gtttattagc agcccgactt
     1321 aacagcggtt ccagcgccac agccagcaac attttcagcg gtcggcgagc aactgatttt
     1381 atcgcccagc ccgccacacc cgccgggcca tagcgcagtg cggtaagaag taccagcttg
     1441 cctgctaatt tgaaaccaag cttcaccttt tgcccggcct gttgccagcg agtattcata
     1501 actttcctca cagttgacgg aaacggctac gtaacgtgaa agtatcggaa gtgacataac
     1561 gttccatctc gcgtaaacgc gtttcacttg ccgccagttc acggtcgact tcatccagca
     1621 attcgctgct ggaaggtagc tgctcaccaa aggccatgtt gtccggcatt ggatcaagcg
     1681 caaatgacaa aatgatgtaa gcaaccaggg taaacagcgc cagaccgaag aaaatcgaca
     1741 gcaccaccag gatacgcacc agttttaccg gtacatcaaa atagttggca atcccggcgc
     1801 agacgccgcg gaccatgccc tgctgtggaa tacgccataa ttttttattg agattaatgc
     1861 ccgccattag cgatccctcc agttcggatg ttctgcatca agaatagatt ccagcgcctg
     1921 aatacgttcg cgcatccgtt ttgcttcatc aaccagttgc gctaatcgct gctgctcact
     1981 ttgcgacaat tcactgcgac cagaacgatt gctgtaatgc agccataacc agatcggtaa
     2041 aacaaacagc acaaaaatgg ttaacggaat agccagaaat agcgcgctca taagtactcc
     2101 ttagacgatg agcggcggcg cgttcggacg ccgcctggat gttattattg attgtcttgc
     2161 ttcattttgg cttttaattg tgccagttgt tcgctgattg catcatcggc tttcagttcg
     2221 gcaaactgat cgtccagcga tttttgttta ccgaagctgt ggctttctgc ttccgcttcc
     2281 atctggtcaa tacgacgttc gaaagattcg aaacgagcca ttgcttcatc cagtttgcca
     2341 ctgtccagct gacgacgcac atcgcgcgac gagtttgccg cctgatgacg taacatcaat
     2401 gcctgctggc gagcgcgtgt ttcgctcaat ttgttttcca gctcgccaat ctctttcttc
     2461 atgcgtgcca gcgtatcgtc caccagcgtc acttcatgtt ccagggactt aatcagatcg
     2521 gtcagtttct gtttttcaat taacgctgca cgtgccagat cctctctctc tttcagcagc
     2581 gccagttcgg ctttttcctg ccattcaact tcacgcgccg acgcttgttc aatacggcga
     2641 gtcagctgtt tcttttctgc caacgcacgc gccgaagtag aacgtacttc aaccagtgta
     2701 tcttccatct cctggatcat cagacgcacc agtttctgtg gatcttccgc tttctctaac
     2761 agagcgttga tgttggcatt cacgatgtcg gcaaagcgag aaaaaatacc cataatgttg
     2821 tcctcttgat ttctgcgttc aggattgtcc tgctgaactg ttaatacaat ttgcgtgcca
     2881 attttttatc tttttgattt ataaagatct gattgaagaa tcaacagcaa catgccagga
     2941 tgaattagcg aattacacta acaagtggcg aatttcatca tggccgaata caaagataat
     3001 ttacttggtg aggcgaacag ctttctcgaa gtgctggaac aggtttcgca tctcgcaccg
     3061 ctggacaaac cggtgctcat catcggcgaa cgcggcaccg gtaaagagtt gattgccagc
     3121 cgcctgcatt atctctcctc ccgttggcaa gggccgttta tttcccttaa ctgcgcggcg
     3181 ttaaatgaaa atctgcagga ttccgaactg tttggtcacg aagcgggggc gtttaccggt
     3241 gcgcaaaaac gtcatcctgg gagatttgaa cgtgccgacg gcggtacgct atttcttgat
     3301 gaactcgcta cggcaccgat gatggtgcag gaaaaattat tgcgtgtgat tgagtacggt
     3361 gaactggagc gcgttggcgg tagccagccg ttgcaggtga atgtgcggtt ggtatgcgcg
     3421 acgaatgccg atctcccggc gatggtcaat gaaggtactt ttcgcgctga cctgctcgac
     3481 cggctggctt tcgatgtagt gcaactgccg cccctgcgcg agcgcgaaag cgacattatg
     3541 ctgatggcag aacactttgc cattcagatg tgtcgggaaa tcaagctgcc tctgttcccg
     3601 gggtttacgg agcgcgccag agaaacattg ctgaattatc gttggccggg aaatattcgt
     3661 gaattgaaaa acgtggtgga acgttcagtg tatcgccacg gcaccagcga ttatccgctt
     3721 gatgacatca ttattgatcc ctttaaacgg cgtccacctg aagaagccat cgccgtttca
     3781 gaaaatacct cgcttccgac gctgccgctg gatttacgcg agtttcagat gcagcaggaa
     3841 aaagagttgc tgcaactcag tttgcaacag gggaaatata accagaagcg cgcggctgaa
     3901 ttactggggt taacttatca tcagttccgc gcgttgttga aaaagcacca gatttagcga
     3961 agttgagcaa tacaacttgc cggatgcggc gtgaatgctt tattcggcat acataatcaa
     4021 tacgtcgttt gcaggcctga taagcgcagc gcatcagaca ttattgcgtt gggctattaa
     4081 tcgctcagcg catcctgcaa aattttcatt gccgcatcga attgcgcatc cgggatggtc
     4141 agcggataca ggaagcgaat cacgttgccg tatgcgccac aggtcagcag gagcagcccc
     4201 tgcgccagcg cgcgttgctg gattttctgt gcaatcgccg ctgacggctc gcccgtttgc
     4261 ggatcgttaa actctgccgc aatcatcgac cccaggccgc gtaccgcagc aatggccgga
     4321 acgctttctt tggcatcaat caacgtgttt ttgagacgct ggcccagttg attcgcgcgt
     4381 tcgcagagtg attctttgtc gataatattg agcaccgcgt gcgcggcagc caccgccagc
     4441 gggttcccgg cgtaggtgcc acccaacccg cccggcgcgg gcgcgtccat aatattcgcg
     4501 ttaccgacca cgcccgaaag cggcatcccg cccgcgaggc ttttcgccat cgtcattaag
     4561 tccggcttat cggcgtaatg atccatggca aacagtttac cggtacgcgc aaagccgctt
     4621 tgcacttcat cggcaatcat cacaataccg tgctcgtcgc acaggcggcg aatggcggca
     4681 accagctctt ttggcgcaac gttgaaaccg ccctcgccct gcaccggttc gaaaataatg
     4741 gccgcgacct gcttcgcttc gatgtctgat ttaaacaagc gttcgatggc gtcgagggag
     4801 tcctgtgttg aaatgccgtg taaatctgac ggataaggta cgtgatacac cgaaccaggg
     4861 aacgggccga agccgatttt gtacggcgca acttttccgg tcagcgccat ggtcatatat
     4921 gtacgaccgt ggaagccacc gctaaacgca atcacgccag gtcgtccggt atgggcgcga
     4981 gcaattttca ccgcgttttc caccgcttcc gcgccggtag tgaagaatgc ggttttagcc
     5041 tgcccgctca ctggggcaag ggcgttaatt ttctccgcca gcgtgacgta gctttcgtac
     5101 ggcacaatct gatacgccgt gtgggtaaac tgttgcaatt gctgctccac cgccgcgacc
     5161 agatcagggt ggcgatgccc ggtattcagc accgcaatgc ctgcggcgaa atcgatgtat
     5221 tcgttgccct ctacgtcctt cagcgtagcg ttttctgccg actgggcgaa gaagttacac
     5281 atcacgccaa ccccgcgtgg ggtagcagaa agacgacgct gatggaattc attgttgctc
     5341 atgtttcaca ccttttgact cttcactgtt aatcggaaat taaacgccca gacgatcgcg
     5401 caggctgtaa taagccgcgc ccatcgcggt aaatggcacg cgcagcgtgc gcccgccggg
     5461 gaacgggtaa tgcggcagat tggcgaaggc atcgaaacgc tcggcgtcac cgcgcagcag
     5521 ttcagcaatc aaacgcccgg caagatgggt acaggtgacg ccgtggccgc tgtaaccctg
     5581 catgtaatag atgttggtat caaggcggcc gaactgcggc atgcgcgaca aggtcagcag
     5641 gaagttgccc gtccagcggt aatcaatttt cacgcccttc aactgaggga aggtttttag
     5701 tagtttcggt accacaagac gctcaacgtc atccgggttg cgtgcaccat acactacacc
     5761 cccgccgtac agcaggcggt tgtcggcggt aagacggtag taatcaagca gatagttaca
     5821 gtcttccaca cagtagtttt tcgggatcag cgaacgggct aaatcttccg acagccgttc
     5881 ggtggtgatc acctgcgtgc cgcacggcat gctgcgtttc gctagttccg gctctacttt
     5941 atcgcccaga tacgcattcc cggcgacaat cacatacttc gccgtcacct gaccattggc
     6001 agtccgaacc acggctggcg tggtgtgctg gatctgcgtc acggcggaaa gctcatacac
     6061 gcgcccgccg ttcaggcgga tggcatcggc ttcaccaatg gcgaggttta gcgggtgaat
     6121 atgcccaccg ctgtgatcca gcagcgcacc agtgtagcga tcgctggcga cctcgcggcg
     6181 aatagcgttg gcgtccagca gctccagctg tttattgccg tagcgttccc agttctcttt
     6241 ctgctcttcg agtgtggcga gttgtttatc attcatcgcc acaaacagac cgccggggcg
     6301 gtagtcgcag tcaatctgat aacgtttgat acgttcgcgg atgatctcgc caccctcgaa
     6361 catcatgctg ccgagcatac gggcggtgtc catgccgtag cttttttcga tcacgtcgat
     6421 gtcgcggctg taggagttca caagctgccc gccattgcgc ccgcttgcgc caaagccgat
     6481 gcgtgaggct tcgagaacca ctacgtcaaa gcccgcttcc gccagatgca gcgcggagga
     6541 gagcccggta tagccgccgc caaccacgca aacgtcgcag gtgattgact cattcagtgt
     6601 gtcgaatggt gcatatttat tcgcactggc ggcgtagtaa ctgctggtat gttcggtcat
     6661 ttcaggcctc caggcttatc cagatggttt tcagttcagt gaatttttca agggcatgca
     6721 gggatttgtc gcgaccgttg ccgctctgct tatagccgcc aaacggcacg gtcatatcgc
     6781 cgtcgttgta gttattgacg aagacggaac cggctttcag gcgtcggctc atgcggtgcg
     6841 cgcgggagag gtcgcgcgtc cataccgccg cgccaaggcc gtactggctg tcgttggcaa
     6901 gctgcagcgc ctgatcttct gatgtgaaac gggtgaccac cagcaccgga ccgaaaatct
     6961 cttcgcggct taaggacgca ttcgggtcca catccacgaa gatggtcggg ccgatggccg
     7021 ctgccagccc ggcgttgcga ccatccagca gcagttgccc cttgccttcg ccttcacgaa
     7081 taaaggtgtg taccgagtca gcgtgagcgc tgtcgattaa ggtgcccatg gtggttgcgg
     7141 gatcaagtgg atggcccggc tgccagtttt gcgcctgctg ttttaacagg gctaagaatt
     7201 catcggcgat gctctcttcc agtaacaaac gcgttccggc gatgcacacc tggccctggt
     7261 tgtagaagat gcctgcggcg gtggcgcttg ccgctttttg cagatccgga caatcggcga
     7321 aaacgatgtt ggcgcttttg ccgcccgctt ccagccagac acgtttcatg ttgctatcac
     7381 ccgcgtcttt cagcagttgt ttacccgtac gcgtggagcc ggtaaaggcg atggcatcga
     7441 tatcgttatg acgcgaaagc gcctgccccg cttcatgacc aaaacccgtc accacgttca
     7501 acacaccatc cggcaagcct gcttctttcg ccagccccgc gagacgaatc gcactgagcg
     7561 gtgatttttc agacggtttt agaatcacgc tgtttcccgc cgccagcgcc gggccgagtt
     7621 tccagcaagt cagcaacagc gggaagttcc acggcacgat ggcggcaatc acgccgaccg
     7681 gttcacgcac gatcatcgcc agctcatggc tactggtggt cgccacttcg ccatacactt
     7741 tgtcgatcgc ttcggcgtac cagcgaatgg cgcgcgccgc gccgggaata tcatcacgca
     7801 gactgtgacg aatcggtttg ccggtgtcga gagtttccag cagtgccagc tcttcggcgt
     7861 gggcttccat taaatcggcg agtttattca gtaccgcttt acgtttagcc ggagaagaga
     7921 gtgaccagtc gccgcgttca aatacgccgc gagctgcgct caccgcacgg tcgatatcga
     7981 cgctcttgcc gcgggcaatt ttcgccagcg gtgcctgggt gaccggatca acggtttcaa
     8041 aggtttcatt ttccgccgca gcagcatatt caccgttaat aaataagcgg ttttcaatgg
     8101 cgagacttaa cgctttatcc tgccagtaag ccagatgatg aaaattcatt atgactcctg
     8161 tttcacgtct atcagatata tgcagtttgt gaacgtcagg gcgcagagat ccccgggctg
     8221 catgcccgtt tatttctggc gacattcttt atggattgcc gggcgcattg cgcttacccg
     8281 gcttacagaa caaaaagatt aaaacgtggt gggcgtatgg gcgctgataa ttcggcaaat
     8341 acctgccgac gtattactga aactgtgcgg gatgccggta ttaatggcat aactttgccc
     8401 cgcgacgagg tggtaatcct gaccattaat cgtcagaaca atttcacctt ccagtactgt
     8461 gcctatttcc tcaccctgat gcttaattct ttccccagtg gttgtgcccg gctggtacgt
     8521 ttcaaagatc atcgccagcg tgcgattcgg gttaccgtta tgaaccagct tcattgacac
     8581 accctgactg cccatctcaa ttaagtcgtc ctgattaatg acgacctgcg gctcatcagg
     8641 tttttccggc tcggaaaaga attccgagag tgacagacca tacaccttca gcagcttttg
     8701 cagcgtactg atggcagggc tgactttatc ttgttctatc gtactgatag cactgtgagt
     8761 cagcccggag agttcggcgg cacgacgttg tgaaagcccc tgctgctggc ggatttccga
     8821 caagcgtttt cctggcgcca gtccctcatc actcatattt gcatttcctt aaactgtagt
     8881 ggtcagagtc gctgtttttc agcgatatgg tgctgccaag cggtgataaa gccctcgaac
     8941 aatatacgcg aaagcgcgta ctcgctactg ttccattccg ggtgccactg tacgcccagc
     9001 gcaaaaggat gattgatgac gctaaccgcc tcaactaagc catccggcga gcgagcttca
     9061 acacgcaacc gtgggctaac gaccttcgcc ccttgtccat gtagagagtt tacccaaaag
     9121 ttgctacatt caggtaacaa cgcagacagt aatcccccct cttcaacctg aacttcatgc
     9181 gacggtgcat attgctgttc caccggcagt tcgggatctt cccgatgctc cagcaattca
     9241 ggctgttcgc acagcttgcg atgcaacgaa ccaccggttg ccaccaccag ttcttgtaaa
     9301 ccccggcaga tggcgaaaat ggggatgcgc ctttcgagtg cggcattaat tatcgccatg
     9361 ctcagaagat cacgcccggg atcggcgtca ggctcatcgc cgttttcacc atatagatgc
     9421 ggctgcacat tgctgggact actaggaaga taaatgccat cgagtttcgg caaaagttgt
     9481 tcaagtaacg acggttccgc cagcgcatgt ggtagcgcaa ttggcaagcc gcctgcgtgg
     9541 atgatggcat tcaggtactt ttcttgcaga gtctgggtcg catgaccctt aagcctgttc
     9601 ctgcacatta cgacaccgat aaccggattg ttcattatat tttccatgct caatctcaca
     9661 aagtggacta aattatcgcc attactgcgc tttaaatgac ctttatgttc aatatttttt
     9721 caatctagca gtggattttc atttttgcaa actaaattta acatttgaca aacattttgt
     9781 ttgcatacag attcgaatga tggtcattat attttacgct ttgataacga gcggaaaaca
     9841 aaccaaaggc gaagaatcat ggaaaccaat atcgttgaag tagagaactt tgttcagcag
     9901 tcagaagaga ggcggggtag cgcctttacg caggaagtga aacgctacct ggagcgctac
     9961 ccgaatacgc aatatgttga tgttttactt actgatttga atggttgctt ccgtggaaaa
    10021 cgtatccctg tttcaagcct gaagaagctc gagaaagggt gttatttccc ggcctcggta
    10081 tttgcaatgg atattctggg caacgtagta gaagaagcag gtctgggtca ggaaatgggc
    10141 gagccggatc gtacctgtgt tcctgttctc ggttccttaa ctccttctgc cgccgatcca
    10201 gagtttatcg atcagatgct cctgaccatg gtcgatgaag atggcgctcc ctttgacgtt
    10261 gagccgcgga acgttcttaa ccgcctgtgg cagcagctgc gccagcgcgg attgttcccg
    10321 gttgtagcgg tagagctgga gttctattta ctggatcgcc agcgcgacgc tgaaggggat
    10381 ctgcaaccgc cctgcgcgcc tggcaccgat gaccgcaata cacaaagcca ggtttactcc
    10441 gttgataacc tcaaccactt cgccgacgtg ctcaatgata ttgatgaact ggcgcagttg
    10501 cagctgattc cggcagatgg cgcggtcgct gaggcctcgc cgggtcagtt tgaaatcaac
    10561 ctttaccata ctgataacgt gctggaagcc tgcgatgatg cgctggcact aaaacgcctg
    10621 gtgcgtctga tggcagaaaa gcataagatg cacgccactt ttatggcgaa gccgtatgaa
    10681 gagcacgcgg gcagcgggat gcatatccat atcagtatgc aaaataatcg tggcgagaat
    10741 gtgctttctg acacagaagg cgaagattcg ccgctgctga aaaagatgct cgccgggatg
    10801 attgacctga tgccgtcgtc gatggcgttg ctggcaccaa acgtgaactc gtatcgccgc
    10861 ttccagccgg gaatgtatgt gccgacgcag gcgtcgtggg gccataacaa ccgcaccgtc
    10921 gccctgcgta ttccgtgcgg tgaccgtcat aatcaccgcg tggaatatca cgtggcgggt
    10981 gccgatgcca acccatatct ggtgatggca gcgatttttg ccggtatttt gcatggcctt
    11041 gataacgagc tgccgttgca ggaagaagtc gaaggcaacg ggctggaaca ggaaggctta
    11101 cccttcccga ttcgccagag cgatgccctg ggtgagttta tcgagaatga tcacctgcgc
    11161 cgctatttag gcgaacggtt ttgccatgtg tatcacgcct gtaaaaatga tgagctgttg
    11221 cagtttgagc gcctcatcac agaaacagaa attgagtgga tgttgaaaaa cgcgtaattc
    11281 cccttgcggg gttgtttcat cggcctgagc ggagccaggc ctgacatttg ctggtaatcc
    11341 agacatttcc cggatgtcgc gttatggagc gcaggcggca acgggcttcc gttaacgatt
    11401 aagactgtga caaggaaatg agatgatgcc gatgattaaa tccccgcatg gcgagggagg
    11461 ctgcgtatgc gcccctcctg ctactgactg gactccgccc cctttgcttc ccttgctaaa
    11521 caggtttgat ttcaggtcga cacgaccgca aaccttatta cgcaggggag gcagcaatta
    11581 tggctattaa ttcaccactg aatattgctg cgcaacccgg caaaacccgt ctgcgaaaat
    11641 cactgaaatt gtggcaggtg gtaatgatgg gtctggccta tctcacgccg atgaccgtat
    11701 ttgatacttt tggtattgtc tccggcatta gcgacggtca cgttccggcg tcctatttgc
    11761 tggcgctggc gggcgtgctg tttaccgcta tcagctacgg caaactggtt cgccagtttc
    11821 cggaggccgg ttcggcctat acctacgcgc aaaagtcgat taacccgcac gtcggattta
    11881 tggtcggctg gtcatcactg ctggattatc tctttttgcc aatgatcaac gttttgttgg
    11941 cgaaaatcta tctctccgcc ctcttcccgg aagtgccgcc gtgggtgtgg gtggtaacct
    12001 tcgtcgccat tttaaccgcc gcgaatctga agagcgtcaa cctggtcgct aacttcaata
    12061 ccctgtttgt actggtgcaa atctccatca tggtggtgtt tatcttcctg gtggttcagg
    12121 gactgcataa aggagaaggc gttggcaccg tctggtcact tcagccgttt atcagcgaga
    12181 acgcgcacct gatcccgatt attaccggtg cgacgattgt ctgtttctcg ttcctcggtt
    12241 tcgatgcggt gaccacgctt tcggaagaga cgccggacgc cgcacgcgtg atcccgaaag
    12301 ccatcttcct gacggcggta tacggcggcg ttatctttat cgcggcgtcg ttctttatgc
    12361 agctgttctt tcccgatatc agccgcttta aagacccgga cgccgcactg cctgaaattg
    12421 cgctgtacgt cggcggcaag ctctttcagt cgattttcct ctgcaccacg tttgtgaaca
    12481 cattagcgtc tggcctggct tcacatgcca gcgtgtcgcg tctgctgtat gtgatggggc
    12541 gtgacaatgt gtttccggag cgcgtgtttg gctatgtgca cccaaaatgg cggactccgg
    12601 cactcaacgt cattatggtc gggattgtcg cgctgtcggc gctgttcttt gatttagtca
    12661 ccgcgacagc actgattaac ttcggtgctc tggtggcgtt taccttcgtg aatctgtcgg
    12721 tgtttaatca tttctggcgg cgtaaaggaa tgaataaaag ctggaaggat cacttccact
    12781 atttgctgat gccgctggtt ggcgcgctga cggtgggtgt gctgtgggtt aacctagagt
    12841 caacgtcact gacactcggt ctggtatggg cttcgttagg cggcgcatat ttgtggtatc
    12901 tgatccgccg ctatcgcaaa gtgccgctgt acgaaggtga cagaacgccg gtgagcgaaa
    12961 cgtaatagcc ggatgcgcgg cgcattatcc tgcctgaggg taacgcgccg cgccattaac
    13021 tataaaaaga tgtatgaccc ggatcacgag tgctatcatc cgcccttatg cttagcgcat
    13081 taaccccagg ggcaaataat gaaccacgat atcccgctaa aatattttga tattgccgat
    13141 gagtacgcga ccgaatgcgc agagcctgtc gcagaggctg aacgcacacc gctggcccac
    13201 tacttccagt tactgctcac ccatttgatg aacaatgaag agatcagcga agaagcccaa
    13261 catgaaatgg ctgctgaagc ggggattaat cccgtacgca ttgatgagat cgcagagttt
    13321 ttgaatcaat ggggtaatga gtaattattt aattgcagac gatcggtcag tatcgttgtc
    13381 cggataaagc gttcacgccg catccggcta acaatgacac gcactgcagc gtgctttaat
    13441 gggtttctca cgatcgtacc gctctgctcc tcgctaagtt gttctgtgag ttatagttat
    13501 agagaaatca cgggtacgaa tgttgaatag atggcgacgc cacctgttca acgaccaacc
    13561 acacggccct cattacgacc cctgaacaca aacggtaatg gcaaatgtca ttaaatatta
    13621 gtaagatagc ctctttacat aaaatccctt aatattatgc gccaggtatt atcgtctctt
    13681 ttggtgattg ctggacttgt gagtggtcag gcaatcgccg cgcctgaatc ccccccgcat
    13741 gctgatatcc gcgacagcgg ttttgtctat tgcgtcagcg ggcaagtcaa cacctttaac
    13801 ccatccaaag cgagcagtgg gttaattgtc gatacccttg ccgcccagtt ttatgatcga
    13861 ctgctggatg tcgatcccta tacctatcgc ctgatgccgg aacttgccga aagctgggaa
    13921 gtactcgaca acggcgcgac ctatcgcttc cacctgcgtc gcgatgttcc gtttcaaaaa
    13981 accgactggt ttactcccac tcgtaaaatg aatgccgacg atgtggtgtt taccttccag
    14041 cgaatttttg accgcaacaa cccgtggcat aacgtcaacg gcagcaactt cccctacttc
    14101 gacagcctgc aatttgccga taacgtgaaa agcgtccgca aactggataa tcataccgtt
    14161 gagttccgac tcgcccagcc ggatgcttct tttttgtggc acctcgcaac ccattatgct
    14221 tcggtcatgt cggcagaata tgcccggaag ttagagaaag aagatcgcca ggagcaactc
    14281 gaccgtcaac cggtcggcac cggtccgtat cagttgtcgg aataccgcgc cgggcaattt
    14341 attcgcctac aacgtcatga tgacttctgg cgcggtaaac cgttaatgcc gcaggtagtg
    14401 gtggatttag gctccggcgg caccggacgt ctgtcgaaac tcctgaccgg ggaatgcgac
    14461 gttctggcct ggcctgctgc cagccagcta tccattttgc gtgacgaccc gcgcttgcgt
    14521 ttaacgctgc gtcctgggat gaacgtcgcc tatctggcat ttaacaccgc caaaccgccg
    14581 ctaaataatc ccgctgtccg ccatgcactg gcactggcga ttaataacca gcgcctgatg
    14641 caatccatct attatggtac ggctgaaacg gcggcctcta ttttaccgcg cgcctcgtgg
    14701 gcctatgaca acgaggctaa aattactgaa tacaatccgg cgaaatcgcg cgaacagttg
    14761 aaggcgttgg ggctggaaaa cttaacgctg aaactgtggg tgcccacacg ttcgcaggcg
    14821 tggaacccca gtccactgaa aactgccgaa ctgattcagg cggatatggc gcaggttggc
    14881 gtaaaagtgg tgattgtgcc ggtagaaggt cgctttcagg aggcgcggtt gatggatatg
    14941 agccatgatc tgacgttatc cggttgggcg acggacagta acgacccgga cagtttcttc
    15001 cgtccgttac tgagctgcgc ggcaattcat tcacagacca acctcgccca ctggtgcgat
    15061 ccgaaattcg acagcgtgtt gcgtaaggcg ctctcctcgc agcagctggc agcgcgtatt
    15121 gaagcctatg acgaagcgca gagtattctg gcgcaggaat tacctattct gccgctggcg
    15181 tcgtcattgc gtttgcaggc ctatcggtac gatatcaaag gtctggtact tagcccgttt
    15241 ggtaacgcct cctttgctgg ggtgtatcgc gagaaacagg atgaggtgaa aaaaccatga
    15301 ttatcttcac cttacgccgc attttgttat tgattgtcac cttgttcctg ctgaccttcg
    15361 ttggcttcag cttgagctat ttcactccac acgcgccgtt acaaggcgcg tcactgtgga
    15421 atgcctgggt gttctggttt aatggcctga tccactggga ttttggtgtt tccagcatta
    15481 acggtcaacc aattgccgaa cagttaaaag aggtcttccc ggcgacgata gaactgtgca
    15541 tcctcgcctt cggttttgcg ctgattgttg gcatccccgt ggggatgatt gccgggatta
    15601 cgcgccataa gtggcaggat aacttgatca acgccatcgc cttactgggc ttttcaatcc
    15661 cggtgttctg gctggcgctt ctgttgacac tgttttgttc actcacgcta ggttggttgc
    15721 ccgtttcagg gcgtttcgat ctgctctacg aagtgaaacc gattaccggt tttgcgttga
    15781 ttgatgcctg gctttcggac tcaccatggc gggatgaaat gatcatgagc gcaatccgcc
    15841 atatgatatt gcccgtgatc accctgtcgg ttgcgccaac aactgaagtg atccgcctga
    15901 tgcgtatcag cactattgaa gtgtatgacc agaactatgt aaaagcggcg gcgacccgcg
    15961 gtttgtcgcg ctttactatt ttgcgtcgcc acgttctgca taacgcgtta ccgccggtta
    16021 ttcctcgcct gggcttacag ttttccacca tgctgacgct ggcgatgatc accgaaatgg
    16081 tttttagctg gccgggttta gggcgctggt taattaacgc catccgccag caggattacg
    16141 cagccatttc cgccggagtg atggtgtgtg gctcactggt cattattgtt aacgtgattt
    16201 ctgatatttt gggtgccatg gctaaccctc tgaaacataa ggaatggtat gccttacgat
    16261 agcgtataca gcgaaaagcg cccgccgggc acgctgcgta ccgcctggcg caaattttat
    16321 agtgacgcct ctgcgatggt cggcctgtac ggctgcgcgg gactggctgt actgtgtatt
    16381 tttggcggct ggtttgcgcc ttacggcatc gaccagcaat ttctcggtta tcaattattg
    16441 ccgccatcat ggtcgcgcta tggcgaagtt tctttcttcc tggggactga cgacctcgga
    16501 cgtgatgttt taagccgttt actgagcgga gctgcgccaa ccgtgggtgg cgcatttgtg
    16561 gtgacgcttg ccgcgacgat ctgtggcctg gtactgggca ccttcgccgg ggcgacacac
    16621 ggcctccgct cagcggtgct taaccatatt ctggatacat tactggcgat cccttcgctg
    16681 ctgttggcaa ttatcgttgt agcgtttgcc ggaccgagtt tgtcccacgc tatgtttgct
    16741 gtctggctgg cattgctgcc gcgtatggta cgttcgattt acagcatggt gcatgacgaa
    16801 ctggaaaaag agtacgttat cgccgcccgt ctggatggcg catcaacgct gaatattctc
    16861 tggtttgctg tgatgccaaa catcaccgcc gggctggtca ccgagatcac ccgcgcactg
    16921 tcgatggcaa ttctcgatat cgccgcgctg ggctttctcg atctcggcgc acaactcccc
    16981 tcgcctgaat ggggagcgat gctcggtgat gcgctggaac tgatttatgt cgccccgtgg
    17041 actgtcatgc tgccaggtgc ggcaattatg attagcgtgt tgttggttaa cctccttggc
    17101 gatggcgttc gtcgtgcgat tattgcgggg gtggaataat gccattactc gatattcgta
    17161 acctgaccat tgaatttaaa accggtgatg agtgggttaa agccgtcgac cgggtaagta
    17221 tgaccttaac cgaaggtgaa atccgcggtc ttgttggtga atccggttcc ggcaagagtt
    17281 tgattgcgaa agcgatttgt ggggtgaata aagataactg gcgtgttact gctgaccgta
    17341 tgcgttttga tgatatcgat ttgctgcgtc tctccgcacg cgaacggcgc aaactggttg
    17401 gccataacgt gtcgatgatt ttccaggaac cgcagtcgtg tcttgaccct tcagaacgtg
    17461 tgggtcgcca gttgatgcaa aacatcccag cctggaccta taaaggccgt tggtggcagc
    17521 gttttggctg gcgcaaacgc cgtgccattg aactgctgca ccgcgtgggg attaaagatc
    17581 acaaagatgc gatgcgcagt tttccctatg agttgaccga aggtgaatgt cagaaagtga
    17641 tgatagccat tgcactggcg aatcaaccgc gtctgctgat tgctgacgaa ccgacaaact
    17701 caatggagcc aacaacccag gcgcaaatct ttcgcctgct gacgcgtctc aaccaaaaca
    17761 gcaataccac tattttgctt atcagccatg acttacaaat gcttagccaa tgggcggata
    17821 aaattaacgt gctttactgc ggtcaaacag tggaaaccgc gccgagtaag gagttggtga
    17881 cgatgccaca tcatccttat acccaggcgc tgatccgcgc gataccagac ttcggcagcg
    17941 cgatgccgca taaaagtcgc ctcaatactc tacccggcgc tatcccattg ctggaacagt
    18001 taccgattgg ttgtcgtctg gggccacgtt gcccgtatgc acaacgagaa tgcattgtga
    18061 cgccacgttt gacgggggcg aaaaatcatc tctatgcctg tcatttcccg ctgaacatgg
    18121 agaaagagtg agatgatcga aacgctgctt gaagtgcgta atttaagtaa aacgttccgc
    18181 taccggaccg gttggtttcg tcgtcagacc gtagaagcgg taaaaccctt gagctttacg
    18241 ctacgtgaag gccagacact ggcgattatt ggcgagaatg gttcgggtaa atccacactg
    18301 gcaaaaatgc tggcgggaat gatagaacct accagcggcg agttattgat tgacgatcat
    18361 ccgctgcatt ttggcgatta ttccttccgt agtcagcgta ttcgcatgat ttttcaggat
    18421 ccttcgacct cgttgaatcc ccgtcaacgt atctcacaaa ttctggattt tccactgcgc
    18481 ctgaacaccg acctggaacc ggaacagcgt cgcaaacaga ttattgaaac gatgcgtatg
    18541 gttgggctgc taccggatca cgtcagttac tatccgcata tgttggcacc cggacaaaaa
    18601 cagcgtctgg gtctggcgcg cgcgctgata ttgcgcccaa aagtcattat tgccgatgaa
    18661 gccctcgctt cactggatat gtcgatgcgt tcgcagttga ttaatctgat gctggagtta
    18721 caggaaaaac agggcatttc gtatatttat gttacccagc atatcggaat gatgaagcac
    18781 attagcgacc aggtgctggt gatgcatcag ggcgaggttg tcgagcgagg cagcaccgcg
    18841 gatgtgctgg catcgccgct gcatgaactc accaaacggc tgattgccgg gcactttggc
    18901 gaagcattga cagcggatgc gtggcgtaaa gatcgctaat tttctgaatc ctccttccag
    18961 attattcacc tcattctgac aagagattaa atcttgtcag aatgaggtga tggataaaat
    19021 aaaatcaaat gcgcgtgatt taagacgcaa tctcactttg caggaacgaa agctctggcg
    19081 atatcttcgc agccgacgtt ttggtgattt caaatttcgc cgtcaacatc cggtggggag
    19141 ctacattctc gattttgctt gctgctcggc gcgcgtagtc gttgagctgg atggtgggca
    19201 gcatgatttg gcagttgcct atgataccag gcgcactagc tggcttgagt cgcagggctg
    19261 gaccgtgctg cgtttctgga ataacgagat tgattgtaat gaggaggcgg tgctggagat
    19321 tattctgcag gaactgaacc gccggtcacc ctcaccctga aagggcgagg gggccgaccg
    19381 agccgaatag ctgttgtggt gaaaacatgg agacagtgct ggagaatatt cggcaaggtc
    19441 tgaaccatcc cagccatcgc cataaaaggg ttaggggctg tatgagcctg tttgttgctg
    19501 gggtaacaat atttgcacaa tatggtcccc tcgcccctct ggggagaggg ttagggtaag
    19561 gggaaaagcg ccccccctgc cgcagcctgc tccggtcgga cctggcaact atagctactc
    19621 acagccaggt tgattataat aaccgtttat ctgttcgtac tgtttactaa aacgacgaat
    19681 cgcctgattt tcaggcacaa caagcatcaa caataaggat taaagctatg ggttttcttt
    19741 ccggtaagcg cattctggta accggtgttg ccagcaaact gtccatcgcc tacggtatcg
    19801 ctcaggcgat gcaccgcgaa ggagctgaac tggcattcac ctaccagaac gacaaactga
    19861 aaggccgcgt agaagaattt gccgctcaat tgggttctga catcgttctg cagtgcgatg
    19921 ttgcagaaga tgccagcatc gacaccatgt tcgctgaact ggggaaagtt tggccgaaat
    19981 ttgacggttt cgtacactct attggttttg cacctggcga tcagctggat ggtgactatg
    20041 ttaacgccgt tacccgtgaa ggcttcaaaa ttgcccacga catcagctcc tacagcttcg
    20101 ttgcaatggc aaaagcttgc cgctccatgc tgaatccggg ttctgccctg ctgacccttt
    20161 cctaccttgg cgctgagcgc gctatcccga actacaacgt tatgggtctg gcaaaagcgt
    20221 ctctggaagc gaacgtgcgc tatatggcga acgcgatggg tccggaaggt gtgcgtgtta
    20281 acgccatctc tgctggtccg atccgtactc tggcggcctc cggtatcaaa gacttccgca
    20341 aaatgctggc tcattgcgaa gccgttaccc cgattcgccg taccgttact attgaagatg
    20401 tgggtaactc tgcggcattc ctgtgctccg atctctctgc cggtatctcc ggtgaagtag
    20461 tccacgttga cggcggtttc agcatcgctg caatgaacga actcgaactg aaataatcgt
    20521 tctgttggta aagatgggcg gcgttctgcc gcccgttatc tctgttatac ctttctgata
    20581 tttgttatcg ccgatccgtc tttctcccct tcccgccttg cgtcaggata acgatttcct
    20641 ttacgaccaa ggagcgccca tggaacaacg ccacatcacc ggcaaaagcc actggtatca
    20701 tgaaacgcaa tccagtacta cggagtatga cgttctgcct ctggtcccgg aagccgcaaa
    20761 ggtcagcgat ccctttctgc tcgacgtgat ccttgatgaa gagacgctgg cccccttcct
    20821 ttcatggctg gtccctgcgc gcgttcttgc agtggagttg ttccctgacc agcttaccgt
    20881 gacccgttca caaaccttca ccgcttatga acgcttgtcg acggccctga cggttgctca
    20941 ggtttgcggc gtccagcggt tatgtaacta ctattcggcg cgacttacgc cgctccccgg
    21001 acctgattcc tccagggaaa gtaatcatcg gttggcacaa atcacgcaat atgcccgcca
    21061 actggctagc tcgcctgcta ttatcgacaa ccgatcgcgc cagcatctga atgacgtcgg
    21121 tcttactgtc tgggactgtg tgatcattaa ccaaatcatt ggttttattg gctttcaggc
    21181 gcggacaatt gcgacatttc aggcttatct cgggcatccg gtacgctggt tacccgggct
    21241 ggcgatacaa aactacgccg acgcgtcact gtttgctgat gaatcaatac gctggcgaag
    21301 cagctatgag gtggaaaaac tacctgaaga gcacacaaaa agttcaactg cagaactttg
    21361 ccaactggcc gaaacacact ctctccaccc tatttcactt tcccttctcg aaaagttgtt
    21421 aaacagcaca cgggtcaata cacagccgga taatcagctt gcggcgttgt tatgcgcgcg
    21481 tataaatggc agtcctgctt gtttttccac ctgtatggat tcatcaaatg aatataaaaa
    21541 aatcagcacc cttctgcgca agggcgaaaa tgaaattaac cgatgggctg accgtcattc
    21601 tgttgagcgc gctaccgttc aggcgataca atggctgacc cgagcacccg atcgctttag
    21661 cgccgcccag ttcagccctt tactcgaaca cgaaaaatca tcaacgcaga ttattaatct
    21721 gctggtatgg agcgggctgt gtggctggat aaatcgctta aaaatcgcgt tgggtgagac
    21781 atattaacct tgccgcgtca gacagattcg cgtaaaactg tcagccgctc taatggccac
    21841 caaaatagac aattatgttt caggacaacc cgctgctagc gcagcttaaa cagcaactgc
    21901 attcccagac gccacgcgct gaaggggtgg taaaagccac agaaaaaggc tttggcttcc
    21961 tggaagtcga cgcacaaaaa agttatttca ttccgccgcc gcagatgaaa aaagtcatgc
    22021 atggcgaccg aattatcgcg gtgatccaca gtgaaaaaga acgtgaatcc gcagagccag
    22081 aagaactggt tgaaccgttc ctgactcgtt tcgtgggtaa agttcagggc aaaaatgacc
    22141 gtctgaccat cgttcctgat catccacttt taaaagacgc cattccttgc cgcgcagccc
    22201 gtggcctgaa ccacgagttt aaagaaggcg actgggcggt tgccgaaatg cgccgtcatc
    22261 cgctgaaagg cgatcgttct ttctatgctg aactgacaca atacatcact tttggcgacg
    22321 accactttgt accgtggtgg gttacccttg cgcgccataa tctggaaaaa gaagcaccag
    22381 acggcgtcgc taccgaaatg ctcgatgaag gtctggttcg tgaagatctg acctcgctgg
    22441 attttgtcac catcgacagt gccagcactg aagatatgga tgacgccctt ttcgctaagg
    22501 cgctgccgga tgacaaactt cagctgattg tggcgattgc cgatccaacc gcgtggattg
    22561 ctgaaggcag caagctggac aaagccgcga aaattcgcgc attcaccaac tatctgcctg
    22621 gcttcaacat ccctatgctg cctcgcgagc tttctgacga tctctgctca ctgcgcgcca
    22681 atgaagtccg cccggtactg gcatgccgca tgacgctctc cgctgatggc accattgaag
    22741 ataatatcga attctttgcc gccaccatcg aatccaaagc gaagctggtg tatgaccagg
    22801 tttctgactg gctggaaaat accggtgact ggaagcctga aagtgaagca attgccgaac
    22861 aagtccgttt gctagcgcaa atttgccaac gccgcggcga gtggcgtcat aaccacgcac
    22921 tagtgtttaa agatcgcccg gattaccgct ttattctcgg tgaaaaaggt gaagtgctgg
    22981 atatcgtcgc cgagcctcgt cgcattgcca accgtatcgt cgaagaagcg atgattgccg
    23041 ctaacatttg tgcggcccgc gtactgcgcg ataagctcgg ttttggcatc tataacgtgc
    23101 atatgggctt tgatccggcg aatgccgacg cgctggcagc gttgctgaaa acgcacggtc
    23161 tgcatgtcga tgccgaagaa gtactcacgc tggacggttt ctgcaaactg cgtcgtgaac
    23221 tggatgcgca accaactggt ttcctcgaca gccgcattcg tcgcttccag tcatttgctg
    23281 aaattagcac tgaacccggt cctcactttg gcctcggtct ggaagcatac gccacctgga
    23341 cttcgccgat ccgtaaatat ggcgacatga tcaaccaccg tctgctgaaa gcggttatca
    23401 aaggcgaaac tgcgacgcgt ccgcaggatg aaatcactgt ccaaatggcc gaacgtcgcc
    23461 gtctcaaccg gatggcagaa cgtgatgttg gcgactggtt atacgcacgc ttcctgaaag
    23521 ataaagccgg gaccggcacc cgtttcgcgg cggaaattgt cgatatcagc cgtggcggca
    23581 tgcgtgtgcg tttggttgat aacggcgcta tcgcctttat tccggcacct ttcttacacg
    23641 ctgtgcgcga tgaactggtt tgcagccagg aaaacggcac tgtacaaatt aaaggtgaaa
    23701 cggtttacaa agtaactgac gttattgacg tcaccattgc cgaagtccgc atggaaaccc
    23761 gcagcattat tgcgcgcccg gtcgcgtaat ctcctttcac ggcccattcc tcatggatgg
    23821 gccgtttatt tccccgctct accttcgtta tatccaggca gattttttaa ctgactttcg
    23881 tttgaaaact ggcgtttttc caaaactgga ttacaaataa atatataaaa aacaatgcgt
    23941 ttatccgccg ttaatttcat gtaagtcatg ttgttccttt tatttaaccg cggactccgc
    24001 tgttaaccgg aggatatgca tcatgaaaac cgttagggag tccacaacgt tgtacaactt
    24061 tctcggatcg cacaatccat actggcggtt gacggaaagc agcgatgctt tgcgcttttc
    24121 taccaccaaa accacagaac ctgatcgtac attgcagtta tctgccgaac aggctgctcg
    24181 catcagggaa atgacggtca tcacctccag cctgatgatg agtctgaccg tcgatgaaag
    24241 cgatctttct gagcatctgg taggacgaaa aatcaataaa cgggaatggg ctggcaacgc
    24301 gtctgcatgg catgacacac cggcggttgc tcgtgattta tcacacgggc tttcctttgc
    24361 tgagcaggta gtttctgaag cacattccgc aatagtgatt ctcgacagcc gggggaatat
    24421 ccaacgcttc aatcggttat gtgaagatta cacagggttg aaagaacacg acgtcattgg
    24481 gcaaagcgtg tttaaactgt ttatgagccg tcgtgaagct gcggcatcca ggcgcaataa
    24541 ccgtgtattt tttcgaagcg gcaatgcata tgaagtcgaa ctgtggatac caacacgtaa
    24601 aggccagcgg ctgtttctgt ttcgcaataa atttgtccac agcggcagtg gcaaaaacga
    24661 gattttttta atctgttccg gtaccgacat taccgaagag cgccgcactc aggagcgact
    24721 gcgtattctg gcaaataccg acagtatcac cggactgccg aatcgtaacg caatgcagga
    24781 gttaatcgat cacgctatta atcaggcaga taacaataaa gttggggttg tgtatcttga
    24841 tttggataat tttaaaaagg tcaacgacgc ctatgggcat ttgtttggtg accagttatt
    24901 acgcgacgtg tcattggcta ttttaagctg tctcgaacat gaccaggtgt tggcgcgtcc
    24961 aggtggcgat gagtttctgg tactggcatc caatacctca caaagcgcgc tggaagcaat
    25021 ggcatcacga attttgaccc gcttacgcct cccctttcgt attggtttaa ttgaagttta
    25081 taccagctgt tcagtaggta tttcgctctc tcccgaacat ggttcggaca gcgcgactat
    25141 tattcgtcac gccgacacag caatgtacac agcgaaggaa ggcggacgag gacaattttg
    25201 cgtttttact ccagaaatga atcagcgggt atttgaatat ctctggctgg ataccaactt
    25261 gcgtaaagca ctggaaaacg atcagttggt tatcgaaaat cacctagcgt ggcgaagtgc
    25321 gcagtctgga agcactagta cgttggcagt cacctgaacg tgggttgatt ccaccgttgg
    25381 acttcatttc ctacgccgaa gagtcagggc taattgtgcc tttaggccgt tgggtgattc
    25441 tcgatgtcgt acgccaggtg gcaaagtggc gggataaagg cataaacctg cgagtggcgg
    25501 taaatatttc tgcacgtcag ctcgccgatc aaaccatttt caccgccctg aaacaggttc
    25561 tccaggaact caattttgaa tactgcccta tagatgttga actgacagag agttgtctga
    25621 ttgagaatga tgaactggca ctgtctgtta ttcaacaatt tagccaacta ggtgcgcaag
    25681 tgcatctgga tgattttggt accggctact cttcactttc gcaactggcg cgctttccga
    25741 tcgatgccat caaacttgac caggtttttg ttcgagatat tcacaaacaa cctgtctcgc
    25801 agtcactggt ccgggcgatc gtcgctgtgg cccaggcatt gaatcttcag gtgatcgccg
    25861 aaggtgtaga gagtgcaaag gaagatgctt ttttaaccaa gaacgggatc aatgagcggc
    25921 aaggattttt gtttgccaaa ccgatgcccg ccgtcgcctt cgaacgctgg tataaacgct
    25981 atctgaagcg cgcataagta gatgaatata tatacttagc gctatatccc gtcattatat
    26041 atcgtattcc tggatgataa ttagccgttg ctgttaaaca cttctctttt agcggcaatt
    26101 tttgcattat tcattttccc ccttttgcta taaggatgtc cttgatgtct gaatttgata
    26161 cccagcgcgt tgccgaacgt attgatattg tgttagatat ccttgtggca ggcgattacc
    26221 actctgcgat ccataatctc gagatactta aagcagaact cttgcgccag gttgctgaat
    26281 ctacgccaga catcccgaag acaccgtggg agatttaaca atcgtgcctt caggttttcc
    26341 tatttgagga tgcccggcgc gtcagtattt ttttatttag tattataacg ttataagaat
    26401 tacagcgatg aattcccgac aacaaactat tctacagatg gtcattgacc agggtcaggt
    26461 tagcgtaacc gatctggcaa aagccactgg agtttctgaa gttaccattc gccaggatct
    26521 caacaccctc gaaaaactga gttacctccg ccgtgcacat ggctttgcag tttcgcttga
    26581 tagtgatgac gtcgaaaccc gtatgatgag caactatacg ctgaagcgtg aactcgccga
    26641 gtttgccgcg tcactggttc aaccgggcga aaccatcttt atcgaaaatg gcagcagcaa
    26701 tgccctgctc gcccggactc tgggggagca gaagaaaaat gtcactatca tcacggtcag
    26761 cagctacatc gcgcatttgc tgaaagacgc gccttgtgaa gtcattttgc tcggtggcgt
    26821 gtaccagaaa aaaagcgaaa gtatggttgg ccctttgaca cgccagtgca tccagcaggt
    26881 acatttcagc aaagcattta ttggtattga tggctggcaa cctgaaactg gattcaccgg
    26941 tcgcgatatg atgcgcaccg atgtggtcaa tgccgtgctg gaaaaagagt gcgaagcgat
    27001 agtcctgact gacagctcga aatttggtgc tgtacattca tactccatcg gtcccgttga
    27061 gcgattcaat cgcgtgatta ccgattcgaa aatacgcgcc agcgatctga tgcatcttga
    27121 gcacagtaaa ctcaccgttc acgtcgttga catttaacca tttcccttct ctacggatga
    27181 tttgcagttt ggcaaatcat ccgctctaag atgattcctg gttgataatt aagactattt
    27241 acctgttatt aacactctca agatataaaa ttattatcag cgatataaca ggaagtaatt
    27301 atcacgtgcg tgatataacc ctgcgcgcga gcagatttca cggaataatt tcaccaggct
    27361 tattcttagc tattatagtt atagagagct tacttccgtg aatcataaat tcaggagaga
    27421 gtattatgtt tgtaacgagc aaaaaaatga ccgcggctgt tctggcaatt actttggcaa
    27481 tgtctctgag tgcctgttct aactggtcta aacgggaccg caacaccgca atcggcgcgg
    27541 gtgcaggggc attaggcggt gcagtactga ccgatggcag tacgttgggt acattaggtg
    27601 gtgcagctgt cggtggtgtt attggtcacc aggtcggtaa ataatcgtaa ataaatgtcg
    27661 attatcgact cgcaattata atacgctt
//
LOCUS       DADMEQ010000046        27499 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.046, whole genome shotgun sequence.
ACCESSION   DADMEQ010000046 DADMEQ010000000
VERSION     DADMEQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.046"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            282..1580
                     /gene="kgtP"
                     /locus_tag="JNZ05_001985"
     CDS             282..1580
                     /gene="kgtP"
                     /locus_tag="JNZ05_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HAY8565225.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1577..1921)
                     /locus_tag="JNZ05_001986"
     CDS             complement(1577..1921)
                     /locus_tag="JNZ05_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HAY8565226.1"
                     /translation="MFFGGHVLRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAI
                     LSAAGNEYSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGA
                     STGYTVWQLTRH"
     gene            complement(1946..3301)
                     /gene="pssA"
                     /locus_tag="JNZ05_001987"
     CDS             complement(1946..3301)
                     /gene="pssA"
                     /locus_tag="JNZ05_001987"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HAY8565227.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3415..6075)
                     /gene="pat"
                     /locus_tag="JNZ05_001988"
     CDS             complement(3415..6075)
                     /gene="pat"
                     /locus_tag="JNZ05_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HAY8565228.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6107..6805)
                     /locus_tag="JNZ05_001989"
     CDS             complement(6107..6805)
                     /locus_tag="JNZ05_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HAY8565229.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGVAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6874..7293)
                     /gene="trxC"
                     /locus_tag="JNZ05_001990"
     CDS             complement(6874..7293)
                     /gene="trxC"
                     /locus_tag="JNZ05_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HAY8565230.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7500..8537
                     /locus_tag="JNZ05_001991"
     CDS             7500..8537
                     /locus_tag="JNZ05_001991"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY8565231.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8585..9274)
                     /gene="ung"
                     /locus_tag="JNZ05_001992"
     CDS             complement(8585..9274)
                     /gene="ung"
                     /locus_tag="JNZ05_001992"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAY8565232.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9579..9962
                     /gene="grcA"
                     /locus_tag="JNZ05_001993"
     CDS             9579..9962
                     /gene="grcA"
                     /locus_tag="JNZ05_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HAY8565233.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10018..10605)
                     /gene="eamB"
                     /locus_tag="JNZ05_001994"
     CDS             complement(10018..10605)
                     /gene="eamB"
                     /locus_tag="JNZ05_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HAY8565234.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10708..11589
                     /locus_tag="JNZ05_001995"
     CDS             10708..11589
                     /locus_tag="JNZ05_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8565235.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11624..12958)
                     /gene="srmB"
                     /locus_tag="JNZ05_001996"
     CDS             complement(11624..12958)
                     /gene="srmB"
                     /locus_tag="JNZ05_001996"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HAY8565236.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13090..13827
                     /gene="trmN"
                     /locus_tag="JNZ05_001997"
     CDS             13090..13827
                     /gene="trmN"
                     /locus_tag="JNZ05_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HAY8565237.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13812..15434)
                     /gene="nadB"
                     /locus_tag="JNZ05_001998"
     CDS             complement(13812..15434)
                     /gene="nadB"
                     /locus_tag="JNZ05_001998"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HAY8565238.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLISGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15690..15845
                     /gene="rseD"
                     /locus_tag="JNZ05_001999"
     CDS             15690..15845
                     /gene="rseD"
                     /locus_tag="JNZ05_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HAY8565239.1"
                     /translation="MLRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15842..16417
                     /gene="rpoE"
                     /locus_tag="JNZ05_002000"
     CDS             15842..16417
                     /gene="rpoE"
                     /locus_tag="JNZ05_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HAY8565240.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIEFLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16450..17100
                     /gene="rseA"
                     /locus_tag="JNZ05_002001"
     CDS             16450..17100
                     /gene="rseA"
                     /locus_tag="JNZ05_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HAY8565241.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17100..18056
                     /gene="rseB"
                     /locus_tag="JNZ05_002002"
     CDS             17100..18056
                     /gene="rseB"
                     /locus_tag="JNZ05_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HAY8565242.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHACLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18053..18532
                     /gene="rseC"
                     /locus_tag="JNZ05_002003"
     CDS             18053..18532
                     /gene="rseC"
                     /locus_tag="JNZ05_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HAY8565243.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18730..20529
                     /gene="lepA"
                     /locus_tag="JNZ05_002004"
     CDS             18730..20529
                     /gene="lepA"
                     /locus_tag="JNZ05_002004"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAY8565244.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20545..21519
                     /gene="lepB"
                     /locus_tag="JNZ05_002005"
     CDS             20545..21519
                     /gene="lepB"
                     /locus_tag="JNZ05_002005"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAY8565245.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21791..22471
                     /gene="rnc"
                     /locus_tag="JNZ05_002006"
     CDS             21791..22471
                     /gene="rnc"
                     /locus_tag="JNZ05_002006"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAY8565246.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22468..23373
                     /gene="era"
                     /locus_tag="JNZ05_002007"
     CDS             22468..23373
                     /gene="era"
                     /locus_tag="JNZ05_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAY8565247.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDIQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23385..24113
                     /gene="recO"
                     /locus_tag="JNZ05_002008"
     CDS             23385..24113
                     /gene="recO"
                     /locus_tag="JNZ05_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAY8565248.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24125..24856
                     /gene="pdxJ"
                     /locus_tag="JNZ05_002009"
     CDS             24125..24856
                     /gene="pdxJ"
                     /locus_tag="JNZ05_002009"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HAY8565249.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24856..25236
                     /locus_tag="JNZ05_002010"
     CDS             24856..25236
                     /locus_tag="JNZ05_002010"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAY8565250.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25656..25736
                     /gene="shoB"
                     /locus_tag="JNZ05_002011"
     CDS             25656..25736
                     /gene="shoB"
                     /locus_tag="JNZ05_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HAY8565251.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25930..26190)
                     /gene="yfhL"
                     /locus_tag="JNZ05_002012"
     CDS             complement(25930..26190)
                     /gene="yfhL"
                     /locus_tag="JNZ05_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HAY8565252.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26405..>27499
                     /locus_tag="JNZ05_002013"
     CDS             26405..>27499
                     /locus_tag="JNZ05_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAY8565253.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSFRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVYEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAV"
BASE COUNT         6784 a         6847 c         7209 g         6659 t
ORIGIN      
        1 ttggcgcaga aaaaaatgcc tgatgcgacg ctgcgcgtct tatactccca catatgacag
       61 attcagcaac ggatgcggtt tccccaactt gcctacttcc atacgtgtcc tccttaccag
      121 aaatttatcc ttaagctcct caataaccat tttcctgcta actaaattca tggtttaggt
      181 tgcataatga tacgcaacaa atgtataaca ttttatttac caaaagaaat aaacaaaagc
      241 gaccaacaaa agcatcggat tacggcagga gacataatgg catggctgaa agtactgtaa
      301 cggcagacag caaactgaca agtagtgata ctcgtcgccg catttgggcg attgtggggg
      361 cctcttcagg taatctggtc gagtggttcg atttctatgt ctactcgttc tgttcactct
      421 acttcgccca cattttcttc ccttccggga acacgacgac tcaactacta caaacagcag
      481 gtgtttttgc tgcgggattc ctgatgcgcc caataggcgg ttggctattt ggccgcatag
      541 ccgataaaca tggtcgcaaa aaatcgatgc tgttatcggt gtgtatgatg tgtttcggat
      601 cgctggttat cgcctgcctc ccaggttatg aaactatagg tacgtgggct ccggcattat
      661 tgcttctcgc tcgtttattt cagggattat ccgttggcgg agaatatggc accagcgcca
      721 cctatatgag tgaagttgcc gttgaagggc gcaaaggttt ttacgcatca tttcagtatg
      781 tgacgttgat cggcggacaa ctgctagccc tactggttgt cgtggtttta caacacacca
      841 tggaagacgc tgcactcaga gagtggggat ggcgtattcc tttcgcgtta ggagctgtgt
      901 tagctgttgt ggcgttgtgg ttacgtcgtc aattagatga aacttcgcaa caagaaacgc
      961 gcgctttaaa agaagctgga tctctgaaag gattatggcg caatcgccgt gcattcatca
     1021 tggttctcgg ttttaccgct gcgggctccc tttgtttcta taccttcacc acttatatgc
     1081 agaagtatct ggtaaatact gcgggaatgc atgccaacgt ggcgagtggc attatgactg
     1141 ccgcattgtt tgtattcatg cttattcaac cactcattgg cgcgctgtcg gataagattg
     1201 gtcgccgtac ctcaatgtta tgtttcggtt cgctggcagc catttttacc gttcctattc
     1261 tctcagcatt gcaaaacgtt tcctcgcctt atgccgcttt tggtctggtg atgtgtgccc
     1321 tgctgatagt gagtttttat acatcaatca gtggaatact gaaggctgag atgttcccgg
     1381 cacaggttcg cgcattaggc gttggtctgt catatgcggt cgctaatgct atatttggtg
     1441 gttcggcgga gtacgtagcg ttgtcgctga aatcaatagg aatggaaaca gccttcttct
     1501 ggtatgtgac cttgatggcc gtggtggcgt ttctggtttc tttgatgcta catcgcaaag
     1561 ggaaggggat gcgtctttag tgacgggtca gttgccagac ggtatagccg gtgcttgcac
     1621 cggcgacatc ccaggccaaa tccttccagc tccagccgct cccttcgggg cggctatccc
     1681 aaagctcttt tgacgccccc aaactgacag agaacatcaa tccaaacatg gcactgcgat
     1741 cccggctcat cccctgatgc tgtgaatatt catttccggc ggcggaaagt atcgccgagg
     1801 cgataaagtg ttgagcttta tcctgcccgc tccagctatc gttagccata tggctgcatc
     1861 cagaaagtaa caatagcgaa cagacaaaaa gaatacgcaa aacgtggcct ccaaaaaaca
     1921 aaccccgtac aggacggggt tgtgattaca ggatgcggct aattaatcgg tcgatgcgga
     1981 tacggcgcaa acggcggatg agtttacgaa ccttcaccgg ataatcggca atactttgca
     2041 gatcgcgata gtgcttaacg atggtggtac gctcgcggat cagctccagt tctttctctc
     2101 gctgcggcgc aagctccagt tgtggatcgt ggatcaaaat ggcgttttcc agatccagac
     2161 gccaggcgcg cgggttcagg ttattaccgg tgatcaacat ccacttatca tcaacccaca
     2221 tccctttcag gtgataggtg ttgtcgtcat ctttccataa ccgaaccact agctggtcag
     2281 tattgacgta atactgcaaa cggctcagga aacgacgcag attgatctca tagagataag
     2341 gcaatgcgcc aattatcttg aaaggttcat cttccggaat gtagaagtcg ttcgcggttt
     2401 tatcaccaac aataatttcg acctttttcc cttcgcgcag caactggata atattgcgca
     2461 caaggattgc tggcaggttg aagtatggcg tacagatggt tagtttctgc tcggcacaag
     2521 gcataagatg gaaaatggtc ttgttcaaca gactcgattt ccccagcccc actagcggcg
     2581 ttacggaaag ctgatcgttg tcggcatcgc cctggaaatg ataagcggca tcacgcagct
     2641 cctggcggaa cagacgaata tcgttcttga tttccgggct ttttggccga ttaacatcat
     2701 ccagacgatt aacgccgcgg ccattcataa tattctgtgt aacccattca aacataatgt
     2761 ctgacatctt acggttacgg atcagatgat aacggtcgta gcgatattta tcgtgttgat
     2821 gcaggtaaac atcgttcagg ctggcaccgc tataaagtac gctatcgtcg atgataaagc
     2881 ctttaaagtg cagaacacca agagcttcac gggtattgat tggaacgcca taaaccggaa
     2941 catctacgcc cggattttcc tgcgccatgc ggcagtacca gtcagcgtta gtgttagatg
     3001 ccgcagcgcc aatgcgtcca cgttgtgcac gatgccagtc gaccagcacc cgcacatcca
     3061 gttccggacg ctgcctttta gcctcataca acgcgttcag aatgcctttg ccaccgtcat
     3121 cctgttcgag atacagggcg acaatgcaaa tgcgctgctt cgcgctggct attttttcca
     3181 gcagcgtctc ccggaagtcg gcgggagcgt aaaagaaatc gacatcatca actgattgag
     3241 aaatcttggg tagttgggca aggtgttgtt gatgtttatt acgcttaaat tttgacaaca
     3301 tcacagtgca tttcttctct gttcattgaa gggtcctctg tgcaatgcag acgacataag
     3361 cgggcaataa taacaccagt cccgattaag tggtcaacat ttccagtacc ttactcatga
     3421 ttcctcgcgc cgggcaagat ttagcgtaag cccaacgatc ccctcttcga gctggatatc
     3481 aacgttaaac ccgagcttgc gggctagcgc caccatgcca cgattgtttg gcatcgtaat
     3541 accattcaga cgttgtagtc cgtgatttcg cgtataggta atcaactttt ccattaagcg
     3601 tcgacctaag cctaaccctt tgagatccga gcgaaccagt acagcaaatt cggcatcgat
     3661 gttatcagga tcggaaatcg cacgcgtgac gccgaggatc tcttccgttt gatcaatacg
     3721 tcgtaccgct acaaacgcca tttcccgatc gtagtcgatc tgtgtcatgt tggctaaatc
     3781 ttcatgggta aattcgttga tctcgctaaa gtagcggtaa taaagatctt ctttggtgac
     3841 tcgcgaaatg aattgctgaa gttgtggctc atcttctggc aaaatcgggc ggaacaagca
     3901 gcgttcaccg tttttcaatt ctacccattc ttccagctga tgcggataag ggcgcactgc
     3961 cagccgactc tcgttatcgc cttcaaacgg cgagatatcc agcgtgacat ccagcgcggt
     4021 aaattcactg ccagaagcca gcaaaggatg aatatccaga cgctgaattt ccgggcaatc
     4081 gacaatcaag ttggaaacct gcaccagaag ctggctcaag cctgcaacat ccaatgggcg
     4141 tagcgcactg cgcgcacgaa tctttttact tttgatcccc tgaataacca gatagcgggc
     4201 caggttcatg ttcagcggcg gcagtgcgac gacggcttga tcttcaggac gccactccac
     4261 accgccttca cccagcatga tcaacggccc gaaaaccgga tcgtgctcaa ccacaacccg
     4321 caactcctga gcgccagcac ggttagccat actttgcacc aacaggccgt ggacccgcgc
     4381 ctgtggccag gccattttta cgcgatcgaa aatagcgttc gccgcttgct ggacttcatt
     4441 ggctgtacgc aggtaaagca tgacgccctg aacttccgat ttatgtggaa tatccggcga
     4501 acgcaatttc agcgccaccg gataaccaat ctgttcggca atatgcaccg cttcggtgct
     4561 atcgctggca atccaggttg ggagcgtgtt catgccatac gcttgcagga tgggctgaac
     4621 ttcatgggta tcgagcgacg tagccccttc ggcaatcgcc tgttgcaaca gaagatgcgc
     4681 ttctgcggta ttggaagtca gattgctggg caacgccggc gtttcgcgta gttgcttctg
     4741 attacgccgg tactccacca tatgcataaa agcagtgatg gttccttccg gggtacggta
     4801 ggtcggcagc ccggcttcgc tgaataaacg tcgtgcctct tgcgaggagt gctcgccgca
     4861 ccagttcgtc agcaaagaga catatttgct gcggggatga tgctttaccg cttcaattaa
     4921 tacttgcgcg ctttctgttg cgggagcagc ggcgctgggc gaatgaataa ccatcagcgc
     4981 atcaaaatcc tggctgtgga gcagaatgtc cagcgttttg acataatgct cgctgctggc
     5041 gtcatcgcgc agatcgagcg ggttagagac tgccacatgt tctggcaatg catcgcgcaa
     5101 tttctggcag gtttcttcgc ttagcgttgc cagcttgcca ttgcgtgacc ataaggcatc
     5161 cagcgccagc gcggcaggcg cagcaccgtt gctgataatc atcaaccggt cgccacgcag
     5221 cgggcgcata tggctaaggg tttccaccgc cgaaaacagt tcgtgggtat cctgtacccg
     5281 caacaaaccg gcacgctgaa tagccgcatc ccatgccggg tccattcctg ccgtggtgtt
     5341 gagcagtcgc tgtgccgccg ggctacgtcc gcttttaatc accagaatcg gtttattacg
     5401 cgaggcacta cgggccgccg aaacaaagcg tcgcgcgtcg cttaactgtt cgagatagag
     5461 caggatggcg ctggttttac tgtcgcgcgc cagatagtcg agcaattcat caacgtcgat
     5521 atccaggctg tcgccgagcg caataaagta ggaaaagccc atctcacgct gttgcgccca
     5581 gtcgaggatg gtgttggaga cggcagccga ttgcgaaata aacgccagct tgccgcgttt
     5641 aatcggcaca ggcgaaaagc tggcattcag accttgccag ggagccagta aacccagact
     5701 gtttggtcca agcaggcgca tgttatggcg cagggcgcag gcgcggagat cttcgtgttg
     5761 cgatgccggg gcggaaagaa taatgcaggt tttacagcct ttctcgccga gctcttccag
     5821 aagagcaaga ttacggctgg cattggtaca taaaaccgca aggtcgggtg taaagggcaa
     5881 gctggcaata tccggccagg ccaacacacc caacaccgct ttccaggctg gcgtcaccgg
     5941 gagtaccggt ccgttaaagc ctcccgccag caggttacgc atcatcaggt aacctgcgcg
     6001 attgggtttc atcgacgcgc caattaccgc tatcgatttt ggtcgcagta gtgcttccag
     6061 tcctcgctga ctcataccgg tctcccgcag aagtgaccga atgattttaa acgctttcta
     6121 actgttctgc tgtgatgcta cccagatgtt gcgtttttcc tgccagatag cgtgttttaa
     6181 agcgggtaaa atgctcgcct aaccctgctg ccaccccggt atcgccggcc atatctaaca
     6241 gtgcgatggc tacctcggca gtacaatatt ggcctgcagc ctgggcttca cgcaggcgat
     6301 aggcagaaag ccgggaaaga tcgacggaaa tgacgggaag attatccaga tacggacttt
     6361 tacgaaacat cttgcgagct tccggccagg taccatcgag catgataaac agcggtggct
     6421 taccggcagg tggtgtgaag atcacttccc gttgctcatc agcatacgag gcgggaaaga
     6481 ccaccattgg ctgataatcc gggttttgta ccagatccag caaatcctgc gagggttcgg
     6541 tacgcgacca ttgaaacgca acggtatcag gcaaaatatc agcaatcaga cgcccggtat
     6601 tactgggctt cattggctcg gtgtcgaaca tcagcaaaca gaagcgactt tttgcttgtg
     6661 ctggggtaat tgtcgaacag agacataatt tctctggcaa aaggcagcgt tggcagcgac
     6721 gaacgcgatt accgcgggca agaaaaggac gtgttgcgcg cgcaatacgc tcggcgcgta
     6781 actggagaac agcgttttcg gtcataagag agcgtcgaaa aaacgccatt gtcgcagagg
     6841 agaaaacggg gcacaagatg cgccccggta agattaaaga gattcgttca gccagctatc
     6901 gaacggcgct ttcggtactg cgccattaag catgtcgaca acctgaccgt ttttgaaaat
     6961 catgatcgtc ggtatactac gaattccaaa gcgactgctc aattcacgtt cagcttcggt
     7021 attcactttc acaaagcgca ctttaccgct acgctcttgc gcgacatctt caaaaattgg
     7081 tgcgaaatta cggcaggggc cgcaccacgg tgcccagaag tcgatcacca caggtagatc
     7141 atccttcagc aatttgtcga gtgtttcacc ggtcgcatta atcacctctc cgtcaaacaa
     7201 gtcgtgaccg cagcgtccgc attttgccgc atcttcgatc cgatcgtcgg gaatgcgatt
     7261 gatggcctga caatgggtac aaacggtatt cataactaac ctcgggatga gtaaggagac
     7321 ttatcggcaa cgacgccggt atgtttctaa tatgttacat attatcgggg acattgttcc
     7381 agacaacaat ctgttttatt caataggtat gatagtcgca ggctttggta cgaaatcatg
     7441 gcgaaccgcg cggacttcgg gtaatctgcg cgcttcgcgc agcgctggtg gagaaaagca
     7501 tgaacgatga aatgaaaggt aaaagcggca aggtcaaagt gatgtatgtc cgcagtgatg
     7561 atgattctga taaacgtacc cacaacccgc gtaccgggaa agggggcggg cgtccaggaa
     7621 aatctcgtgc tgatggtggc cgtcgccccg cccgcgatga caaacagagt cagccccgtg
     7681 accgcaagtg ggaagattcg ccgtggcgca cggtttcccg cgcgccgggt gatgagacgc
     7741 cggaaaaggc cgatcacggt ggcatcagtg gtaaaagttt tattgatccg gaagtgttgc
     7801 gtcgtcagcg tgcggaagaa acccgcgtct acggcgaaaa cgcctgtcag gcactgttcc
     7861 agagccgtcc ggaagcgatt gttcgcgcct ggtttatcca gagtgtaacg ccgcgtttta
     7921 aagaagcctt gcgctggatg gcagcaaacc gcaaagcgta ccatgtggtg gatgaagcgg
     7981 aattgacaaa agcgtcaggc acggaacatc acggcggcgt ttgcttcctg atcaaaaagc
     8041 gtaacggtac aaccgtgcag cagtgggtaa tccaggcagg cgcgcaggat tgcgttctgg
     8101 cactggaaaa cgaatctaac ccgcataacc tgggtggcat gatgcgcagc tgcgcgcact
     8161 tcggcgtgaa aggtgtcgtg gtgcaggatg cggcactgct ggagtcgggg gcggctatcc
     8221 gtaccgcaga aggcggcgca gagcacgttc agccgattac tggcgacaac attgttaacg
     8281 tgctggatga tttccgtcag gcaggttaca ccgtagtgac cacttccagc gagcagggta
     8341 aaccgctgtt caaaaccagt ctgccagcga aaatggtact ggtgctgggt caggaatatg
     8401 aagggttacc ggatgccgca cgcgatccga acgatctgcg cgtgaagatt gacggtacgg
     8461 gcaacgttgc cgggctgaat atctctgtcg caaccggcgt tttgcttggt gaatggtggc
     8521 gtcagaataa agcctgataa atcagccggg tggcaactct gccatccggc atttccccgc
     8581 aaatttactc actctctgcc ggtaatactg gcatccagtc aatcggcgtc tcgccacgtt
     8641 gttccagcca ctgatttgcc agcacaaaat ggttgcagcc aaagaatcca cgatgcgccg
     8701 aaagcggcga cggatgcggt gctttcagta catgatggcg ttgcttatct ataatcgccc
     8761 ctttcttttg cgcatgtgat ccccacaaca aaaacaccac gccttcgcgg tgctggttaa
     8821 tcaggctgat gactttatca gtaaacgtct cccaaccgag gctggcgtgg gaatgcgcct
     8881 gacctgcgcg aaccgtcaac acagtattga gcagcagaac gccctgacgc gcccagcttt
     8941 cgagatagcc atgattaggg cgggtgaagc ccggaatagt attttccagc tctttataca
     9001 tattcaataa tgacggagga atggcaatgc cgggacgaac ggaaaatgcc agaccatgcg
     9061 cctgtcccgg tccgtgataa ggatcctggc cgagaatcac cactttaacg tcacccaact
     9121 cggtaaagcg gaacgcgtta aagacatctt tttgtggtgg atagatagtg acgccggact
     9181 gccgctcgct ggcgacggtc tgaagggtat taagaaaata aggttgttgc ttctcttcag
     9241 ccagcacgtc atgccaggtt aattcgttag ccatcttgct ctcctgcgaa tcttcaatcc
     9301 gcctagctta actgcttctt tctacagaac aaaatcatgc tcatttttta gacttaaacg
     9361 agtgcaaaaa aatgtaaatt ggtaaaattt cacacccgca taagttgatg taaaacaata
     9421 aaatccattt acgaccacaa ttgatgtgtg gtttttgttg atttaaatca aagaatcaag
     9481 ggtggttgag gagtatatat acactcaagc aacaatggtt ttaccaattg gccgcgacag
     9541 gctgaacaaa tcaaataatt ttgccgggga ggcatcacat gattacaggt atccagatta
     9601 ctaaagccgc taacgacgat ctgctgaact ctttctggct gctggacagc gaaaaaggcg
     9661 aagcacgttg catcgttgca aaagcaggtt atgcagaaga tgaagtggtt gcagtaagca
     9721 aactgggtga cattgaatac cgtgaagttc cagtagaagt gaaaccagaa gttcgcgttg
     9781 aaggtggtca acacctgaac gttaacgttc tgcgtcgtga aactctggaa gatgcagtta
     9841 agcatccgga aaaatatccg cagctgacca tccgtgtatc cggttatgca gttcgcttta
     9901 actctctgac tccggaacag cagcgcgacg ttatcgctcg tacctttact gaaagcctgt
     9961 aaggcttaag tagcaagtta ctaagcggaa gagggcgacc tcttccgctt tttttcgtta
    10021 atagaaaatg cgtaccgcgc aatagaccag caacagggca agcacgatat ttaactggcg
    10081 accatactgg cgaaacaatc gctgaaacag atgccccgcc agcgcccagc acacattgcc
    10141 aaacgtccca atcatcgcca gcaaaacgct gacgccaact acccagctta gcgcctgtgt
    10201 ttgcggcaga acaaacgtcg acagtgccgt aacaccgtac aaaatgattt tgacgttcac
    10261 aaactgcaaa gcaaagctgg cccaaaagct gattggtttt gcctgaagtc cgtcttcctt
    10321 tgttgggctg gtggcgattt tccacgccag ccagacaata tatgccgccc ccgcccaact
    10381 caaaaggtgt accgctgccg ggtcaatcac tgccagtgaa aatgaaatgc ccgcacacag
    10441 taacatcaca atcaaaaatc ccagactcat ccctgccagc acgcgggtac tttgacgaaa
    10501 tccatgcgac gtagcagagc taagggcgag aatattgttc ggtcctggcg tcatagcggt
    10561 aatcagggtg taagtccaaa aagcacttaa aaggatcggt gtcacttgtt atctcctctg
    10621 ttttggtaga cagtacccga tacggaacat ttaaaaaaat gaatgttttt atattctaaa
    10681 ttcgatattt tcgataggtt tggggttatg gatctgcgcc gttttattac gcttaaaacc
    10741 gtggtggaag agggttcctt tttgcgagct tcgcaaaaat tgtgctgtac acaatcgacg
    10801 gtgacttttc atattcagca gcttgagcag gagttttcag tccagttatt tgagaaaatt
    10861 ggccgacgga tgtgccttac ccgcgaagga aaaaagttgc tgccgcacat ttatgaactt
    10921 acccgagtga tggatacgct tcgtgaggct gccaaaaagg agtcagatcc ggacggggaa
    10981 ctacgcgttg tttcgggcga aacgttgctc tcttaccgaa tgccgcaggt gttgcagcgt
    11041 tttcggcaac gcgcaccaaa agtgcgttta tcgttgcagg cgctgaactg ttacgtgatc
    11101 cgtgatgcgc tactgaatga tgaggccgat gtcggcgtct tttatcgcgt agggaatgat
    11161 gatgccctga accgacgaga gttgggtgaa caatcgctgg tgctggtggc ttcaccacaa
    11221 attgcagatg tcgattttac tgagccggga agacataacg cctgtagctt tattatcaac
    11281 gaaccgcaat gtgtcttccg gcagatattt gagagcacgc tgcgacagcg gcggatcacg
    11341 gtggaaaaca ccattgagct tataagtatc gaaagcatca agcgttgtgt cgcggcgaat
    11401 atcggtgtca gttacttgcc ccactttgcg gtggaaaaag agcttgagag cggagaatta
    11461 attgaattgc cttttggcga acagtcgcag accattacgg caatgtgcgc acatcatgct
    11521 ggaaaagcgg ttagcccggc gatgcacatt tttattcagt gtgttgaaga gtgttttgtg
    11581 gttgcgtaaa aaaacaatgc cgggcttaaa cccggcattg agattactct tctgtcgttt
    11641 gcggtggcac gcccgttccg ctcggtttac ggcgcttacc aatatttttg gtgtcgcgat
    11701 ggcgtttttt cacccgcggc ttctctttct ctttagcttt tttcttctcg gcgcgttttg
    11761 ccagtacttt cttcgatggc ttgccggtct gcttctcgct tggcgcacgc gttttcgggc
    11821 gtaactcatc aataacgcga gctttaatcg gctcttcaat gtaacggcca accttgccca
    11881 gcagcaaatg gtcatgggct tccaccagcg aaattgcggt gcctttacga ccggcgcgcg
    11941 cggtacgtcc gatacgatgc aaataagtat cgccactgcg cggcatatcg aagttaaaga
    12001 cgtggctgac gtcaggaatg tcgataccgc gcgcggcaac atcggtggca accagtacgt
    12061 ttacgcggcc ttcggtcagg cgtttaattg cttcgttacg cttgccttgt accatctcac
    12121 cttcgagata gcagttgttg atgcccgctt cgcgcagcca gtttgccagc tcatgcacac
    12181 gttcacgctt acgcacaaac acgattgagc gagtcgcttc aggctgtttt aacaggtgca
    12241 ccagcaatgc ggttttatgt tccaggtcat cggcgcggta ataccactga tgaatttttt
    12301 tgcgctcacg ggtggacgga ttggcagaaa cttccaccgg atcttccagc agacgctcgg
    12361 caaagtcctg aatcgcatcg ccttccagcg ttgccgaaaa gagcagggtc tgtttacgcc
    12421 agcgagtttc gccagcaata tgttcgatat cctgagcgaa gcccatatcc agcatacggt
    12481 ctgcttcgtc gaggatcagc gtttcaaccg cgcggcaatc gaagttctct tcttttatgt
    12541 attgcagcag acgtccggtc gtggcgacca cgatgtcctg attttcgctg aacacttccg
    12601 cgtggttcat ataggctacg ccgccggtga tggtggcgat atccagatgc gtatgtttcg
    12661 ccagttcgcg ggcatgatcg gccacctgca tcgccagctc gcgagttggg gtgaggatca
    12721 aaatacgcgg cggaccggat ttcttacgtg ggaaatcgag caggtgctgc aacgctggca
    12781 gcagatacgc cgccgtttta ccggtgcctg tcggcgcaga accgagtaca tcacggccat
    12841 cgagcgcagg cggaatggcg gcagcctgaa tggcggtcgg gcgagtgaaa cctttatcct
    12901 ggagggcttc cagcaggctt tcgtcgagtt caagttcgga aaaagtcgtt acagtcatgt
    12961 tctacctctg tgtggggcgc tgattataga cgttacggct gcaatcttca tctgtttgta
    13021 tggatatcgc ttctcgggta ttgttctcac ccggtgctat cgccgttttt cctgcaaggt
    13081 tttattttca tgtcacagtc tacatccgtg cttcgtcgta atggatttac ttttaaacag
    13141 ttttttgttg ctcacgatcg ctgtgcgatg aaagtgggaa cggatggcat tttgctgggc
    13201 gcatgggcac cggtggctgg ggtgaaacgt tgccttgata tcggcgcggg tagcgggttg
    13261 ctggcactga tgctggcgca gcgaaccagt gacagcgtga taattgatgc cgttgagctg
    13321 gaaagtgaag ctgccgcgca ggcgcaggaa aatatcaacc agtccccgtg ggcagagcgg
    13381 attaacgtcc atacggcgga tattcagcag tggatcacac agcagacagt acgcttcgat
    13441 ttaatcatca gtaacccacc ttactatcag cagggagtag agtgcgcgac acctcaacgg
    13501 gaacaggctc gctataccac tacgcttgat cacccatcgc tgctgacctg tgcggcggag
    13561 tgcattaccg aagagggatt tttctgcgtg gtgctgccag agcagattgg taatggtttt
    13621 actgaactgg cattaagcat gggctggcat ttacgtttgc ggacggatgt ggcggaaaac
    13681 gaagcgcgac tgccgcatcg ggtgctgctg gcattctccc cgcaggcggg agaatgcttt
    13741 agcgatcgct tagtcattcg tgggccagac cagaactatt ccgaagcgta tacggcgctg
    13801 acccaggctt tttatctgtt tatgtaatga ttgccggggg aaaggatcga tggaccggaa
    13861 tgcgtgagca gttccgggta atccagcgta aaatgcagcc cccggctttc tttgcgcatc
    13921 atcgcgcagc gaacaatcaa ctcggcaacc tgtaccagat tacgcagctc cagcaaatta
    13981 tttgagacgc ggaaatgggc gtaatattcg tctatttctt gttggagcat ggttatccgc
    14041 cgcagggcgc gttccaggcg cttcgttgtg cgcacaatgc caacgtaatc ccacataaac
    14101 agccgtagct cgtgccagtt atgctgaatt actacccgtt cgtcagggtt ctcaacgcgg
    14161 ctttcatccc acggcggtaa cgtactgacg ccgtgggcat aaggcatacg tctggtgata
    14221 tcttctgccg ccgaccagcc atagaccaga cactccagca atgaattcga ggccatgcgg
    14281 ttagcgccgt gtaagccggt ataactcacc tcgccaatgg catacaagcc ctcgacgtcc
    14341 gtacgcccat gatcatcaac cattacacca ccgcaggtat agtgtgcagc aggcacaatc
    14401 ggtaccggtt cttgtgtgag atcaatcccc agcccgagca atttttcata aatcatcggg
    14461 aaatgctggc gaataaaatc ggcgggctta tggctgatgt cgaggaacat acaatctgcg
    14521 ccgaggcgtt tcatttcatg gtcaatggcg cgggcgacaa tatcgcgcgg ggccagttcg
    14581 ccgcgctcat caaaatcggg cataaaacgc gtgccatccg ggcgcttgag ataagcgcct
    14641 tcgccgcgca gtgcttccgt taacaggaaa ttgcgtgcct gtgggtgata cagtgcggta
    14701 gggtggaact gattaaattc gagattggca acccgacagc ccgcgcgcca cgccatagca
    14761 atgccatcgc cagaagaaat atccggattg gtggtgtact gatacacctt cgacgcaccg
    14821 ccggttgcca gtaccactgc ttttgcgtgg caggtttcca ccgtttcttt atttcggttc
    14881 catacccacg cgcctacaac ccgtcgcgtg cccggcaggc caattttgtc agagacaatc
    14941 agatcaactg cgttgctgcg ctccagcaca cgaatattcg gatggttctg cgccttgctc
    15001 accagcgtgg attgtacttc tctaccggtg gcgtcggcgg catgaagaat acgacggtga
    15061 ctatgtccac cttcacgggt cagatggtag ctttcttcgc cattcggttg aacgtgggta
    15121 tcaaacaaaa ccccctggtc aattaaccat tgcacacagg aacgagcatt gctggcgaca
    15181 aattcaactg catggcgatc gcaaatacca gccccggaaa tcaatgtgtc ttccacatgc
    15241 gagtcaatgc tgtcagtttc atcaaacacg gcggcaatac cgccctgggc ataaaatgtt
    15301 gaaccttccg ttaccgggcc tttacttaga acgatgacct gatgctggtc agccaggcgt
    15361 agcgccagtg aaagtccggc tgcgccgcta ccaataatca acacgtcaca tgaatgttca
    15421 gggagagtat tcattttctt tgtttaattt actaaacatt gtttggtcag catagcatca
    15481 tgttgtgcga ataaacacct gctattttaa tatttgttac agtggctaaa cacgttgacg
    15541 cagggcggcg agaaaaagag aagttactgg ctggtggagg attaggtggt gaaataaaaa
    15601 ggccgttggg ttactcttca ggcagttaaa tgggcatttc tacacagata atgcgatgtt
    15661 cagattctgt agacttataa tgatagataa tgctccgtct acagcatgac aaacaaaaac
    15721 agatgcgtta cggaacttta caaaaacgag acactctaac cctttgcttg ctcaaattgc
    15781 agctaatgga gtggcgtttc gatagcgcgt ggaaatttgg tttggggaga ctttacctcg
    15841 gatgagcgag cagttaacgg accaggtcct ggttgaacgg gtccagaagg gagatcagaa
    15901 agcctttaac ttactggtag tgcgctatca gcataaagtg gcgagtctgg tttcccgcta
    15961 tgtgccgtcg ggtgatgttc ccgatgtggt acaagaagct tttattaaag cctatcgtgc
    16021 gctggattcg ttccggggag atagcgcttt ttatacatgg ctgtatcgga ttgctgtaaa
    16081 tacagcgaaa aattacctgg ttgctcaggg gcgtcgtcca ccttccagtg atgtggatgc
    16141 cattgaagct gaaaacttcg aaagtggcgg cgcgttgaaa gaaatttcga accctgagaa
    16201 cttaatgttg tcagaagaac tgagacagat agttttccga actattgagt tcctcccgga
    16261 agatttgcgc atggcaataa ccttgcggga gctggatggc ctgagctatg aagagatagc
    16321 cgctatcatg gattgtccgg taggtacggt gcgttcacgt atcttccgag cgagggaagc
    16381 tattgataac aaagttcaac cgcttatcag gcgttgacga tagcgggata ctggataagg
    16441 gtattaggca tgcagaaaga acaactttcc gctttaatgg atggcgaaac gctggatagt
    16501 gagctgctta acgaactggc tcataaccca gaaatgcaga aaacctggga aagctatcac
    16561 ttaatccgtg attcaatgcg gggtgatact cccgaggtgc tccatttcga tatctcttca
    16621 cgcgtgatgg ccgccattga agaagagcca gtacgtcaat cggcaacatt gatcccggaa
    16681 gcccagcctg cgccgcatca atggcagaaa atgccattct ggcagaaagt acgtccgtgg
    16741 gcggcacagc ttacccaaat gggcgtagcc gcatgcgtat cgcttgcagt tatcgttggc
    16801 gtccagcact ataatggaca atctgaaacg tcccagcagc ccgaaacgcc ggtatttaat
    16861 acactgccga tgatgggtaa agccagcccg gtaagcctgg gagtaccttc tgaagcgacc
    16921 gcaaacaatg gtcaacagca gcaggtacag gagcagcgtc gtcgcattaa tgcaatgttg
    16981 caggattacg aactgcaacg ccgactccac tctgaacagc ttcagtttga gcaggcgcaa
    17041 acccagcaag ccgctgtaca ggtgccagga attcaaactt taggaacgca atcgcagtaa
    17101 tgaagcaact ttggtttgcc atgtcattag tgacaggtag cctgttattc tctgctaacg
    17161 cctcggccac tcccgcgtcc ggggcgttat tacagcagat gaacctggcc agtcagtcac
    17221 tgaattacga gctgtcattc atcagcatca ataaacaggg tgttgagtct ctgcgttatc
    17281 gacatgcatg cctcgataac cgtcctcttg cacaattgtt gcaaatggat ggcccgcgcc
    17341 gggaagtggt acagcgcggc aatgaaatca gctattttga accgggactt gaaccgttca
    17401 cgcttaatgg cgattacatt gttgattctc tgccatcgct tatctatact gatttcaaac
    17461 gcctttctcc ttactacgac tttatctccg tcgggcgcac gcgtattgct gatcgtcttt
    17521 gcgaagtcat tcgcgtggtt gcccgagatg gtacacgcta cagctacatc gtgtggatgg
    17581 acaccgaatc gaaattaccg atgcgggttg atctccttga tcgcgatggt gaaacgctgg
    17641 aacaatttcg cgtgattgct tttaacgtca atcaggatat cagcagcagt atgcagacgc
    17701 tggcgaaggc aaatttgccg ccgttgcttt ctgttcctgt aggtgaaaaa gctaaattca
    17761 gctggacgcc aacctggttg ccacagggtt ttagcgaagt ttccagtagt cgacgtccgc
    17821 taccgacgat ggacaacatg cctatcgaat cacgtctcta ttccgacgga ttattcagct
    17881 tctcggtaaa cgttaaccgc gctacgccat cgagcaccga tcagatgttg cgcaccggac
    17941 gcagaaccgt cagtacaagc gtacgtgata acgccgaaat caccattgtc ggtgaactgc
    18001 cgccgcaaac ggcgaaacgc attgccgaga atattaagtt cggggcagcg caatgatcaa
    18061 agagtgggct accgtcgtct cctggcaaaa cgggcaggcg ctggtcagtt gtgatgttaa
    18121 agcttcatgc agcagctgcg cttcacgcgc cggttgcggt agccgcgtgt tgaataaact
    18181 tggcccgcaa accacgcata ccattgtcgt accctgtgat gagccgttag tgccggggca
    18241 aaaagtggaa ttggggatcg ccgaaggcag cctgcttagc tccgcattac tggtttatat
    18301 gtcgccgctg gtgggattat tcctcatcgc ttcgctattt cagctactct ttgcttctga
    18361 cgtcgcagca ttatgtggtg cggtcctcgg cggtattggc gggttcctga ttgcgcgcgg
    18421 ctactcgcgt aagtttgctg cccgggcaga atggcaaccg atcatcttaa gcgtggcatt
    18481 gccgcctggc cttgtgcgat ttgaaacatc ttcggaagac gcgagccagt gatttcactg
    18541 gctttgttgc attttgcata acaaatgcga ggacgtttcc agataccaca atccatacgg
    18601 tatgaacatt tcctcgcctc aatgttgtag tgtagaatgc ggcgtttcta ttaatacaga
    18661 cgttaagctc agaacagcga cttctaaaag cctggttaac cgggcattaa ggcacaataa
    18721 tcatacttta tgaagaatat acgtaacttt tcgatcatag ctcacattga ccacggtaaa
    18781 tcgacgctgt ctgaccgtat tatccagatc tgcggtggcc tgtctgaccg tgaaatggag
    18841 gcgcaggttc tcgattccat ggatcttgag cgtgagcgtg gcattaccat caaagcgcaa
    18901 agcgtgacgc tggactacaa agcgtctgac ggcgaaacct atcagcttaa ctttatcgac
    18961 accccgggcc acgtagactt ctcctatgaa gtttcccgtt cgctggctgc ctgtgaaggt
    19021 gcattgctgg tggtcgacgc cgggcagggc gtagaagcgc aaaccctggc aaactgctac
    19081 accgccatgg aaatggatct cgaagtggtg ccggtactga acaagattga cctgccggca
    19141 gccgatcctg aacgcgtggc ggaagaaatt gaagatatcg tcggcatcga cgccaccgat
    19201 gcagtgcgct gttcagcgaa aaccggcgtt ggcgtgcagg acgttctcga acgtctggtg
    19261 cgcgacattc cgccgccgga aggcgatccg gaaggcccgt tgcaggcact aattatcgac
    19321 tcctggttcg acaactacct gggcgttgtt tcacttatcc gtattaaaaa cggcaccctg
    19381 cgtaagggcg acaaagtgaa agtcatgagt accgggcaga cctataacgc cgaccgtctg
    19441 ggcatcttca cgccgaaaca ggttgaccgc actgaactga aatgtggcga agtaggctgg
    19501 ctcgtatgtg cgattaaaga tatccacggc gctccagtcg gcgatacctt aacgctggcg
    19561 cgtaatccgg cagaaaaggc gctgcctggc tttaagaaag tcaaaccgca ggtatacgcc
    19621 ggtctgttcc cggtaagttc cgacgactat gaagccttcc gtgacgcgct gggtaaactc
    19681 agcctgaacg atgcctcact gttctatgag ccggaaagct ccagcgcgct gggctttggt
    19741 ttccgctgcg gcttccttgg cctgctgcac atggagatca tccaggagcg tctggaacgt
    19801 gaatacgatc tggatctgat caccactgcg ccgaccgtag tgtatgaagt tgaaaccacg
    19861 tcaagggaag ttatctacgt cgacagccca tccaagctgc ctgcggtaaa taacatttac
    19921 gaactgcgcg agccgattgc agagtgtcac atgctgctgc cgcaggcata tctcggcaac
    19981 gttattacgc tgtgcgtaga aaaacgcggc gtgcagacca atatggttta ccacggtaat
    20041 caggtggcgc tgacgtacga gatcccgatg gcggaagtgg tgctcgactt cttcgatcgc
    20101 ctgaaatcta cctcgcgtgg ttatgcgtct ctggattaca acttcaaacg cttccaggcg
    20161 tccgacatgg tacgtgtaga cgtattaatc aacggtgaac gtgttgatgc gctggcgttg
    20221 atcacccacc gtgataattc gcaaaaccgc ggtcgcgagt tggtggagaa gatgaaagat
    20281 ctgatcccac gccagcagtt tgatatcgcc attcaggcag cgattggtac gcacattatt
    20341 gcgcgatcca ccgtgaaaca gctgcgtaaa aacgtactgg ctaaatgtta tggcggcgat
    20401 atcagccgta agaaaaagct gctgcagaag cagaaagaag gtaagaaacg catgaagcag
    20461 atcggtaacg ttgagctgcc gcaggaagcg ttcctcgcca ttctgcacgt cggcaaagac
    20521 aacaaataac ccttaggagt tggcatggcg aatatgtttg ccctgattct ggtgattgcc
    20581 acactggtga cgggcatttt atggtgcgtg gataaattct ttttcgcacc taaacggcgg
    20641 gaacgtcagg cagcggcgca ggcggctgcc ggtgactcac tggataaagc aacgttgaaa
    20701 aaggttgcac cgaagcctgg ctggctggaa accggagctt ctgtttttcc ggtgctggct
    20761 atcgtattga ttgtacgttc gtttatttat gaaccgttcc agatcccgtc aggttcgatg
    20821 atgccgactc tgttaatcgg tgattttatt ctggtagaga agtttgctta tggcattaaa
    20881 gatcctatct accagaaaac gctgatcgaa accggtcatc cgaaacgcgg cgatatcgtg
    20941 gtctttaaat atccggaaga tccaaagctt gattacatca agcgcgcggt gggtttaccg
    21001 ggcgataaag tcacttacga tccggtctca aaagagctga cgattcaacc gggatgcagt
    21061 tccggccagg cgtgtgaaaa cgcgctgccg gtcacctact caaacgtgga accgagcgat
    21121 ttcgttcaga ccttctcacg ccgtaatggt ggggaagcga ccagcggatt ctttgaagtg
    21181 ccgaaaaacg aaaccaaaga aaatggaatt cgtctttccg agcgtaaaga gacactgggt
    21241 gatgtgacgc accgaattct gacagtgccg attgcgcagg accaggtggg gatgtattac
    21301 cagcagccag ggcaacaact ggcaacctgg attgttccgc cgggacaata cttcatgatg
    21361 ggcgacaacc gcgacaacag cgcggacagc cgttactggg gctttgtgcc tgaagcgaat
    21421 ctggtcggtc gggccacggc tatctggatg agcttcgata agcaagaagg cgaatggccg
    21481 actggtgtgc gcttaagtcg cattggcggc atccattaat agccatcttc gttcacgttg
    21541 tcgccgttat ggcgacaacg tgaattattt atgagataaa tctcccgtgg ctaacgacat
    21601 cccccgtcgt tgtgtataga atattccccc gaagtttaag gttggcacct ccaggttgcc
    21661 acggcacacg aaacagcgtt ggtccccata taccgctaaa ctgaaactgc agcgaagcag
    21721 ttagcagagc catgtatatc aggtctgttt cgtgtgctga attgttgacg catttattta
    21781 ttggtatcgc atgaacccca tcgtaattaa tcggcttcaa cggaagctgg gctacacttt
    21841 taatcatcag gaactgttgc agcaggcatt aactcatcgt agtgccagca gcaaacataa
    21901 cgagcgttta gaatttttag gcgactctat tctgagctac gttatcgcca atgcgcttta
    21961 tcaccgtttc cctcgtgtgg atgaaggcga tatgagccgg atgcgcgcca cgctggtccg
    22021 tggcaatacg ctggcggaac tggcgcgcga atttgagtta ggcgagtgct tacgtttagg
    22081 gccaggtgaa cttaaaagcg gtggatttcg tcgtgagtca attctcgccg acaccgtcga
    22141 agcattaatt ggtggcgtat tcctcgacag tgatattcaa accgtcgaga aattaatcct
    22201 taactggtat caaactcgtc tggacgaaat tagcccaggc gataaacaaa aagatccgaa
    22261 aacgcgcttg caagaatatt tgcagggtcg ccatctgccg ctgccgactt atctggtagt
    22321 ccaggtacgt ggcgaagcgc acgatcagga atttactatc cactgccagg tcagcggcct
    22381 gagtgaaccg gtggttggca caggttcaag ccgtcgtaag gctgagcagg ctgccgccga
    22441 acaggcgttg aaaaaactgg agctggaatg agcatcgata aaagttactg cggatttatt
    22501 gccatcgtcg gacgtccgaa cgttggcaaa tccacattgt tgaacaaact gctggggcag
    22561 aaaatctcca tcacctcccg caaggcgcag acaactcgtc accgcattgt ggggatccat
    22621 actgaaggcg cgtatcaggc gatctacgtc gatacaccgg gcctgcatat ggaagaaaaa
    22681 cgcgccatta accgcctgat gaacaaagcg gcgagcagct ctattggcga tgttgagctg
    22741 gtgatttttg tcgttgaagg cacccgctgg acgccggacg acgaaatggt gctcaacaaa
    22801 ctgcgcgaag gcaaagcgcc ggtaatcctc gcggtgaaca aagtggacaa cgtgcaggag
    22861 aaagccgatc tgctgccgca cctgcagttc ctggcaagcc agatgaactt cctcgatatc
    22921 gtgccaatct ctgccgaaac cgggctgaat gttgacacta ttgcggcaat cgtgcgtaag
    22981 catctgcctg aagcgactca tcacttcccg gaagattaca tcaccgatcg ctcacagcgt
    23041 tttatggcgt ctgaaatcat ccgcgaaaaa ctgatgcgtt tccttggcgc tgaactgccg
    23101 tactccgtga ccgtggagat cgaacgtttc gtctctaacg aacgcggcgg ttatgacatc
    23161 aacggtttga ttctcgttga gcgtgaaggg cagaagaaga tggttattgg caacaaaggg
    23221 gccaagatca aaaccatcgg gattgaagcg cgtaaagaca tacaggaaat gttcgaagcg
    23281 cctgtccacc ttgaactgtg ggtaaaagtg aaatcgggtt gggccgacga cgaacgcgca
    23341 ctgcgcagtc tcggttatgt tgacgatctt taagagtcac tccgatggaa ggctggcagc
    23401 gcgcatttgt cctgcatagt cgcccgtgga gcgaaaccag cctgatgctg gacgtcttca
    23461 cagaggaatc gggtcgcgtg cgtctggttg ccaaaggcgc acgctctaaa cgctctactc
    23521 tgaaaggtgc attacagcct ttcacccctc tcttgctacg ttttggcggg cgtggcgaag
    23581 tcaaaacgct gcgcagtgct gaagctgtct cactggcgct gccattaagc ggtatcacgc
    23641 tttacagcgg tctgtacatc aacgaacttc tctcccgcgt actggaatac gagacgcgct
    23701 tctctgaact ctttttcgat tacttgcact gcattcagtc tcttgcaggt gccactggta
    23761 cgccagaacc cgcgctgcgt cgctttgaac tggcactact cgggcatctg ggctatggcg
    23821 tcaattttac ccattgcgcg ggtagcggcg agccggtaga tgacaccatg acgtatcgtt
    23881 atcgcgaaga aaaagggttt atcgcaagcg tcgttatcga caataaaacg ttcactggaa
    23941 ggcagttaaa agcgttaaac gcgcgggaat ttcctgacgc agacacacta cgcgccgcga
    24001 aacgctttac ccgaatggcg cttaagccgt atcttggcgg taaaccatta aagagcaggg
    24061 aactgttccg gcagtttatg cctaagcgaa cggtgaaaac acattatgaa tgatgaggat
    24121 tgtcatggct gaattactgt taggcgtcaa cattgaccat attgcaacgc tgcgtaacgc
    24181 acgcggtact gcttacccgg atccggtgca ggcggcgttt attgccgagc aggcgggagc
    24241 ggacggcatt accgtgcatt tacgtgaaga tcgccgccac attactgacc gcgatgtgcg
    24301 catcctgcgg cagacgctgg atacccgaat gaatctggag atggcagtga ccgaagagat
    24361 gctggcgatc gccgttgaga cgaagccaca tttttgctgc ttggtaccgg aaaagcgtca
    24421 ggaagtaaca accgaaggcg gcctggacgt tgcagggcag cgtgaaaaaa tacgcgatgc
    24481 ctgcaaacgt ctggcagatg ccgggattca ggtttctctg tttatcgacg ccgatgaaga
    24541 gcagatcaaa gctgcggcag aggttggcgc accgtttatc gagatccaca ccggttgcta
    24601 tgctgatgcc aaaactgacg ccgaacaggc gcaagagctg gtgcgtatcg ccaaagccgc
    24661 gacctttgcc gctagtctcg gtctgaaagt taacgccgga cacggtctga cctatcacaa
    24721 cgtgaaagcc attgccgcca tccctgagat gcatgaactg aatatcggtc atgccattat
    24781 tggccgtgca gtgatgaccg gactgaaaga tgcggtggca gaaatgaagc gtctgatgct
    24841 ggaagcgcgt ggctaatggc aatattaggt ttaggcacgg atatcgtaga gatcgctcgc
    24901 atagaagcag tgatcgcccg atccggtgag cgcctggcac gccgcgtatt aagcgataac
    24961 gaatgggcaa tctggaaaac gcaccaccag ccggtgcgtt ttctggcgaa gcgttttgct
    25021 gtgaaagaag ccgcagcaaa agcgtttggc accgggatcc gcaatggtct ggcgtttaat
    25081 caatttgaag tattcaatga tgagctcggc aaaccacggc tacgactatg gggtgaggca
    25141 ttaaaactgg cggaaaagct cggcgttgta aatatgcatg taacgctggc agatgaacgc
    25201 cactatgctt gtgccacggt aattattgaa agttaaaata ataccgttct ctttaaaaag
    25261 taaaaccccc gaactggtaa ggaacggggg ttttactttt taactttaat tttgtgcagc
    25321 atgcaccctc aacaacagcg attatggatc agtgtactta tcttttacaa gcaacttgca
    25381 atctttagca taaaaactcg agcctttacg aagaaagcaa tattgatgga aagattaacg
    25441 tgaccgccaa ttcgtaagta cattaaaatt ggcttcgtta ttgaagattt tgctgtgctt
    25501 tacaccatgc cacagaattc ccccattgaa acgagtggtg tcgtcaaagc tctggtgtgg
    25561 agtgcagcat gcaccctcaa taactcgcac gttcagtttt ggggagatgt aagggctaat
    25621 ctgaatggct gcattccttg tttaaggaaa aacgaatgac tgattgccga tacctgatta
    25681 aacgggtcat caaaatcatc attgctgttt tacagctgat ccttctgttc ttataacaca
    25741 aggaaacgta cttaaggtgc gtccggtgaa ccaatcggac gcgcctttaa taactataaa
    25801 taagtgtctt ggcagatact atataaatta acttagtgaa tgattatgct aatgtcatca
    25861 attaaataaa tataatggcg ttaaggcttc ccagtaatat aattaatact ctacttccag
    25921 agtagaatat taaattttat ccgcgtggtg catcagcaca aatttatccc acaactgttc
    25981 ttctgtctcg acatgcgccg gatctttcac aatagtattg gggatcgggc acaccttctg
    26041 gcaggttggt gtctcgtagt gccctacgca ttcggtacac ttatcgctgt taatttcgta
    26101 gatatgatct cccattgaaa tcgcctcatt cgggcattcg ggttcacaca tatcacaatt
    26161 aatacagcgt ttagtaatta gtaaagacat ttcaatggat taccattaaa tcattttaaa
    26221 atcagtaagt tgtatcgagt ttgtatgctt tactgtcatt aacttactgt atgttgatcc
    26281 agtgtatttc accttgataa actcagtcca gcaacacaaa accgcaacac attgcatttt
    26341 gtcccgtaga aaagacttgt atgtgtgagc ttgttttctg cgcctacgca gataaggatt
    26401 gagaatgccg cgcactgtaa cacataatcc ggatagcccc aataatgacg atgttttagc
    26461 cgcatctgaa aaatgggacg cctgtaaacc cccctatacc agcgcacaca tgaaaatctg
    26521 tgttgctgcc gccaaaatca tcctcgctgc ttccggcgtg gctcgccgtt ccaaatacga
    26581 aaaagagaac tatctccgta tcgatttcag caaagccggt aaggttacat tttacgccga
    26641 gtttccaaaa aagatgggcc tcaaaggtaa aaagctcggc gagtggccgg agctcgctat
    26701 ccagctggcg cgcgaaaaag cactaggtat ggctgacggt ggactgcggg cagagtccgt
    26761 acatgcagcg ctggaaatgt accgggatga cctcaaagcc aaagtagccc ggcagaagct
    26821 gagcccggac agtttcacaa cctacggggt gcgtatcgac cggattaaag caacgttcgg
    26881 cgagcgtgag gtgttcagcg acgtaacata caatcggctg gtggaagtgc tggacgagtg
    26941 gatcgccact cgctcgaaca ataacgccct ggagttgttt gccgagctcc gtcggttctg
    27001 gaagttctgc gcacctactc tttgcaacgg ccgcaatgtt gccgccagtc tgccagatga
    27061 ttatgtttcc ttccgcgtac agaaacctac ccccacacgg ctttttaccg atattgaatc
    27121 aatcgcccga ctctggctca atgttgctgc ctgcacctct gtacaccaga agaatgctgt
    27181 tcgcttcatg atcatcactg gtgttcgtcc gattaatgtc cataacctgc gctgggacta
    27241 cgtttacgag gaggctggtg aaattgttta tccggaaggg gttatcggca tgcgaggggc
    27301 tatgaaaaca caaaaggctt tccgcctgcc gataacgcct gagatccggc ggattatcga
    27361 cgagcagaaa gcctggcgtg attcagttcc tgagtgcaac agggattatg tatttttgca
    27421 gccacgtgat ccaatgcagc cattttcaaa acgatcactg gataagctgg tgaaaacata
    27481 cagcccggac ggggctgta
//
LOCUS       DADMEQ010000047        27127 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.047, whole genome shotgun sequence.
ACCESSION   DADMEQ010000047 DADMEQ010000000
VERSION     DADMEQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27127
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.047"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..437
                     /locus_tag="JNZ05_002014"
     CDS             <1..437
                     /locus_tag="JNZ05_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HAY8565254.1"
                     /translation="IIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAPAPQV
                     ELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVETLAWF
                     TVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(499..1119)
                     /gene="rhtB"
                     /locus_tag="JNZ05_002015"
     CDS             complement(499..1119)
                     /gene="rhtB"
                     /locus_tag="JNZ05_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HAY8565255.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            1230..2252
                     /gene="pldB"
                     /locus_tag="JNZ05_002016"
     CDS             1230..2252
                     /gene="pldB"
                     /locus_tag="JNZ05_002016"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HAY8565256.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWHQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            2260..3060
                     /gene="yigL"
                     /locus_tag="JNZ05_002017"
     CDS             2260..3060
                     /gene="yigL"
                     /locus_tag="JNZ05_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HAY8565257.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            3136..4035
                     /gene="bioP"
                     /locus_tag="JNZ05_002018"
     CDS             3136..4035
                     /gene="bioP"
                     /locus_tag="JNZ05_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HAY8565258.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(3923..4876)
                     /gene="metR"
                     /locus_tag="JNZ05_002019"
     CDS             complement(3923..4876)
                     /gene="metR"
                     /locus_tag="JNZ05_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HAY8565259.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            5112..7373
                     /gene="metE"
                     /locus_tag="JNZ05_002020"
     CDS             5112..7373
                     /gene="metE"
                     /locus_tag="JNZ05_002020"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAY8565260.1"
                     /translation="MTILNHTLGFPRVGLRRELKKVQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(7412..8227)
                     /locus_tag="JNZ05_002021"
     CDS             complement(7412..8227)
                     /locus_tag="JNZ05_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY8565261.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            8489..9250
                     /gene="udp"
                     /locus_tag="JNZ05_002022"
     CDS             8489..9250
                     /gene="udp"
                     /locus_tag="JNZ05_002022"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HAY8565262.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            9391..10818
                     /gene="rmuC"
                     /locus_tag="JNZ05_002023"
     CDS             9391..10818
                     /gene="rmuC"
                     /locus_tag="JNZ05_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HAY8565263.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPGQPNDEAYQRDDEYNQQSR"
     gene            10913..11668
                     /gene="ubiE"
                     /locus_tag="JNZ05_002024"
     CDS             10913..11668
                     /gene="ubiE"
                     /locus_tag="JNZ05_002024"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HAY8565264.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            11682..12287
                     /gene="ubiJ"
                     /locus_tag="JNZ05_002025"
     CDS             11682..12287
                     /gene="ubiJ"
                     /locus_tag="JNZ05_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HAY8565265.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            12284..13924
                     /gene="ubiB"
                     /locus_tag="JNZ05_002026"
     CDS             12284..13924
                     /gene="ubiB"
                     /locus_tag="JNZ05_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HAY8565266.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            14003..14272
                     /gene="tatA"
                     /locus_tag="JNZ05_002027"
     CDS             14003..14272
                     /gene="tatA"
                     /locus_tag="JNZ05_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HAY8565267.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            14276..14791
                     /gene="tatB"
                     /locus_tag="JNZ05_002028"
     CDS             14276..14791
                     /gene="tatB"
                     /locus_tag="JNZ05_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HAY8565268.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            14794..15570
                     /gene="tatC"
                     /locus_tag="JNZ05_002029"
     CDS             14794..15570
                     /gene="tatC"
                     /locus_tag="JNZ05_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HAY8565269.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            15612..16394
                     /gene="tatD"
                     /locus_tag="JNZ05_002030"
     CDS             15612..16394
                     /gene="tatD"
                     /locus_tag="JNZ05_002030"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HAY8565270.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(16391..16879)
                     /gene="rfaH"
                     /locus_tag="JNZ05_002031"
     CDS             complement(16391..16879)
                     /gene="rfaH"
                     /locus_tag="JNZ05_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HAY8565271.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            17046..18539
                     /gene="ubiD"
                     /locus_tag="JNZ05_002032"
     CDS             17046..18539
                     /gene="ubiD"
                     /locus_tag="JNZ05_002032"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HAY8565272.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVTYIDAIWDELAIFNNGKSA"
     gene            18585..19286
                     /gene="fre"
                     /locus_tag="JNZ05_002033"
     CDS             18585..19286
                     /gene="fre"
                     /locus_tag="JNZ05_002033"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HAY8565273.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(19379..20542)
                     /gene="fadA"
                     /locus_tag="JNZ05_002034"
     CDS             complement(19379..20542)
                     /gene="fadA"
                     /locus_tag="JNZ05_002034"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HAY8565274.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARIHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(20552..22741)
                     /gene="fadB"
                     /locus_tag="JNZ05_002035"
     CDS             complement(20552..22741)
                     /gene="fadB"
                     /locus_tag="JNZ05_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HAY8565275.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEITAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            22931..24262
                     /gene="pepQ"
                     /locus_tag="JNZ05_002036"
     CDS             22931..24262
                     /gene="pepQ"
                     /locus_tag="JNZ05_002036"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HAY8565276.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVITLPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLGLIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            24262..24876
                     /locus_tag="JNZ05_002037"
     CDS             24262..24876
                     /locus_tag="JNZ05_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HAY8565277.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            24915..26366
                     /gene="trkH"
                     /locus_tag="JNZ05_002038"
     CDS             24915..26366
                     /gene="trkH"
                     /locus_tag="JNZ05_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HAY8565278.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPAINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            26378..26923
                     /gene="hemG"
                     /locus_tag="JNZ05_002039"
     CDS             26378..26923
                     /gene="hemG"
                     /locus_tag="JNZ05_002039"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HAY8565279.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHAMRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         6229 a         6905 c         7473 g         6520 t
ORIGIN      
        1 tgattatcga aaaaatggcc tggctgcata cgctgattat ggtgggcggt ggcctgtatc
       61 tctgctggat gggttaccag atgctacgcg gtgcactgaa aaaagaggct gtatctgcgc
      121 ctgcaccaca ggttgagctg gcgaaaagcg ggcgcagttt cctgaaaggc ttactgacca
      181 atctcgccaa tccgaaagcg attatctatt ttggttcggt attctcattg tttgtcggcg
      241 ataacgttgg cactaccgag cgctggggca ttttcgcgtt gattattgtc gaaacactgg
      301 cgtggtttac cgtcgttgcc agtctgtttg ccctgccgca aatgcgccgt ggttatcaac
      361 gtctggcgaa gtggattgat ggttttgctg gggcgttatt tgccggattt ggcattcatt
      421 tgattatttc gcggtgatgc cagacgcgtc ttcagagtaa gtcggataag gcgtttacgc
      481 cgcatccgac attatttttc acgcatgcct cgccgatgct aacagcgctc ccaccagcat
      541 aaacaacgag ccgaaaatct tattcagcgc cttcatctgc tttggtcctt taatccatag
      601 agcaatcctt tgagcaaggg tggcgtaacc gatcatcaca ataatatcga ccacaatagt
      661 ggtgacgccg agcacgatat actgcatcag ttgcggctgt tgcggcatga tgaattgcgg
      721 aaatagcgcc gccagaaaca caatactttt gggattggtg agattcacaa aaactgcgcg
      781 ctggaacaaa tgtcgacgcg attgagtaga ggccagcgat ttaagatcaa ttgcgccagc
      841 ggcgcgccac tgctggattc ccagccaaat caagtaagcc gcgcctgccc actttaacac
      901 ttcaaacgca atcactgagc gggaaaatag cgtccccaac cccacgccaa ccagcacaat
      961 atgaatcgcc agtccggtct gaagcccagc aatagacgcc accgcgccgc gataaccgtg
     1021 gttgagcgag gtggtcatag tgttgattgc accagagcct ggcgacaggc ttaaaatgat
     1081 cgatgtcagc aggtaggcaa accaccattc taaggtcatg atgaactccc ggtgtgtcta
     1141 tttttgtgcc acaatacgct actgtcgcag cgttgtgtca ggcacgctta aaaaaacgat
     1201 tttacgtggt ttaagaggca gattacccga tgtttcagca gcaaaaagac tgggaaacaa
     1261 gagaaaacgc gtttgctgct tttaccatgg gaccgctgac tgatttctgg cgtcagcgtg
     1321 atgaagcaga gtttactggt gtggatgaca ttccggtgcg ctttgtccgt tttcgcgcac
     1381 agcaccatga ccgggtggta gtcatctgcc cggggcgtat tgagagctac gtaaaatatg
     1441 cggaactggc ctacgacctg ttccatttgg ggtttgatgt cttaatcatc gaccatcgcg
     1501 ggcagggacg ttccggtcgc ctgttagccg atccgcatct cgggcatgtt aatcgcttta
     1561 atgattatgt tgatgatctg gcggcattct ggcatcagga ggttcagccc ggtccgtggc
     1621 gtaaacgcta tatactggca cattcgatgg gcggtgcgat ctccacatta tttctgcaac
     1681 gccatccagg tgtatgtgac gccattgcgc taactgcgcc aatgtttggg atcgtgattc
     1741 gtatgccgtc atttatggca cggcagatcc tcaactgggc cgaagcgcat ccacgtttcc
     1801 gtgatggcta tgcaataggc accgggcgct ggcgcgcgtt gccgtttgct atcaacgtac
     1861 tgacccacag cagacagcga tatcgacgta acttacgctt ctatgctgat gacccaacga
     1921 ttcgcgtcgg tgggccgacc taccattggg tacgcgaaag tattctggct ggcgaacagg
     1981 tgttagccgg tgcgggtgat gacgccacgc caacgcttct cttacaagct gaagaggaac
     2041 gcgtggtgga taaccgcatg cacgaccgtt tttgtgaact ccgcaccgcc gcgggccatc
     2101 ctgtcgaagg aggacggccg ttggtaataa aaggtgctta ccatgagatc ctttttgaaa
     2161 aggacgcaat gcgctcagtc gcgctccacg ccatcgttga ttttttcaac aggcataact
     2221 cacccagcgg aaaccgctct acagaggttt aaatttctta tgtaccaggt tgttgcgtct
     2281 gatttagatg gcacgttact ttctcccgac catacgttat ccccttacgc caaagaaact
     2341 ctgaagctgc tcaccgcgcg cggcatcaac tttgtgtttg cgaccggtcg tcaccacgtt
     2401 gatgtggggc aaattcgcga taatctggag attaagtctt acatgattac ctccaatggt
     2461 gcgcgcgttc acgatctgga tggcaatctg atttttgctc ataacctgga tcgcgacatc
     2521 gccagcgatc tgtttggcgt agtcaacgac aatccggaca tcattactaa cgtttatcgc
     2581 gacgacgaat ggtttatgaa tcgccatcgc ccggaagaga tgcgcttttt taaagaagcg
     2641 gtgttcaaat atgcgctgta tgagccggga ttactggagc cggaaggcgt cagcaaagtg
     2701 tttttcacct gcgattccca tgaacaactg ctgccgctgg agcaggcgat taacgctcgc
     2761 tggggcgatc gcgtcaacgt cagtttctct accttaacct gtctggaagt gatggcgggc
     2821 ggcgtgtcga aaggccatgc gctggaagca gtggcgaaga aactgggcta cagcctgaag
     2881 gattgtattg cgtttggtga cgggatgaac gacgccgaaa tgctgtcgat ggcggggaaa
     2941 ggctgcatta tgggcagtgc gcaccagcgt ctgaaagatc tgcatccgga gctggaagtt
     3001 attggtacta atgccgacga cgcggtgccg cattatctgc gtaaactcta tttatcgtaa
     3061 tcgttcttta tttggtcagt tgtcaacctg atacttcgct acaatggata ctcgttaatc
     3121 aaagagtttt ccattgtggc gctacttatc atcaccacga ttctgtgggc cttctccttt
     3181 agcttttatg gcgagtacct tgcggggcac gtcgatagct attttgcggt gctggtgcgc
     3241 gttggcttgg cggcactcgt ttttctgccg tttctgcgta cccgtagcaa tagcctgaaa
     3301 acggtcggcc tgtatatgct ggtgggcgcg atgcagctcg gcgtgatgta tatgctgagt
     3361 ttccgcgctt atctctacct gacggtctcc gaattgttgc tgttcaccgt actgacgccg
     3421 ctctacatca cgctgattta tgacatcatg agtaagcgcc gtctgcgctg gggctatgct
     3481 tttagcgcct tgctggcggt gattggtgcc gggattattc gctatgatca ggtcaccgac
     3541 catttctgga ctggcttgtt gctggtgcaa ctctccaata tcacttttgc catcggcatg
     3601 gtgggttaca aacgcctgat ggaaactcgc ccgatgccgc agcataacgc ctttgcgtgg
     3661 ttctatcttg gcgcgttcct ggtggcggta attgcatggt tcttgctggg aaatgcgcag
     3721 aaaatgccgc aaaccacgtt gcaatggggc attctggtgt ttcttggcgt ggtggcttcc
     3781 gggattggct actttatgtg gaactacggc gcgacgcagg tggacgccgg aacgctgggc
     3841 attatgaata atatgcacgt tccggcaggg ctgctggtaa acctggctat ctggcaccaa
     3901 cagccgcact ggccaacgtt tattacaggc gcgctggtga tcctggcctc actgtgggtg
     3961 catcgtaagt gggtcgctcc gcgctcttca caaacggcag atgatcgcag gcgtgattgc
     4021 gcgctgagcg aataaacgct tccgtaactg gctgacgctg ctcgccatcg cgcacggcgg
     4081 cgtacagtcg gctccacaag ccttcgccca gggttttggt caccaccaga ccctggcgct
     4141 caaaactctc tactacccaa tgcggtagcg cggcaatacc catccgcgcg gcaaccattt
     4201 gaatcaataa taaggtgtta tcgacgcttt tcagtgacgg gctgacgcct gccggctgaa
     4261 gaaaatgccg ccagacatcc agtcgactac gctgcaccgg ataaattaat agcgtctcgc
     4321 tggcgagatc ttccggtgta atacgcgttt ttgccgccag tggatggtca ggtgctaaca
     4381 ccaggcgtac ttcataatcg aacatcggcg aataatgcag gccactgcgc ggcaggatat
     4441 cggaggtcat caccagatcc agctcgccct gttgcaacgc gggctgcggg tcaaacgtca
     4501 cgcccgattt aaaatccatc tctacctgcg gccagttctt atggaaattt tctaaagcgg
     4561 gtgtcagcca ctgaatacag ctatggcact caatggcaat gcgcagacgc gtctgctgcg
     4621 gttcattgca ggcttgcagt gcctggctga tttgcggcag cacctggttt gccagttgca
     4681 acaggatttc tccctgcggt gtaaagcgta gcggctggct cttacgcaca aatagccgga
     4741 agccaaggcg ttgttccaga tcgctaaact ggtgagacag ggcggattgc gtctgatgca
     4801 acgttgccgc agcggctgcg agcgagccgc agttccgcaa cgcttgtagc gttttcaggt
     4861 gttttacttc gatcatgaaa gtccttcact tcggcatgaa taatttgcgc ttgaggaata
     4921 tacagtaacc gccaattatg gatgtgtaaa catctggacg gctaaaatcc ttcgtctttt
     4981 aaatttatgg tgcgttggct gcgtttctcc accccggtca cttacttcag taagctcccg
     5041 gcgatgaata aacttgccgc cttccctaaa ttcaaaatcc ataggattta catataatta
     5101 gaggaagaaa aatgacaata ttgaatcaca ccctcggttt ccctcgcgtt ggcctgcgtc
     5161 gcgagctgaa aaaagtgcaa gaaagttatt gggcggggaa ctccacgcgt gaagaactgc
     5221 tgacagtagg gcgtgaactg cgtgctcgtc actgggatca acaaaagcaa gcgggtatcg
     5281 acctgctgcc ggtgggcgat tttgcctggt acgatcatgt actgaccacc agtttgctgc
     5341 tgggtaatgt tccgccacgt catcagaaca aagacggttc ggtagatatc gacaccctgt
     5401 tccgtattgg tcgtggacgt gcgccgactg gcgaacctgc ggcggcagcg gaaatgacca
     5461 aatggtttaa caccaactat cactacatgg tgccggagtt cgttaaaggc caacagttca
     5521 aactgacctg gacgcagctg ctggaggaag tggacgaggc gctggcgctg agccacaacg
     5581 ttaagcctgt gctgctgggg ccggttactt acctgtggct ggggaaagtg aaaggtgaac
     5641 agtttgaccg ccttagcctg ctgaacgaca ttctgccggt ttatcagcaa gtgctggcag
     5701 aactggcgaa acgcggcatc gagtgggtac agattgatga acccgcgctg gtactggaac
     5761 taccacaggc gtggctggac gcatacaaac ccgcttacga cgcgctccag ggacaggtga
     5821 aactgctgct gaccacctat tttgaaggcg taacgccaaa tctcgacacg attactgcgc
     5881 tgcctgttca ggggctgcat gtggaccttg tacatggtaa agatgacgtt gttgaactgc
     5941 acaagcgcct gccttctgac tggctgctgt ctgcgggtct gatcaatggt cgtaacgtct
     6001 ggcgcgccga tcttactgag aaatatgcgc aaattaagga cattgtcggc aaacgtgatt
     6061 tgtgggtggc atcttcctgc tcgttgctgc acagccccat cgacctgagc gtggaaacgc
     6121 gtcttgatgc agaagtgaaa agctggtttg ccttcgccct acaaaaatgc catgaactgg
     6181 cactgctgcg cgatgcgctg aacagtggtg acacggcagc tctggcagag tggagcgccc
     6241 caattcaggc gcgtcgtcac tctactcgtg tacataatcc ggcggtagaa aagcgtctgg
     6301 cggcgatcac cgcccaggac agccagcgtg cgaatgtcta tgaagtgcgt gctgaagccc
     6361 agcgtgcgcg ttttaaactg ccagcgtggc cgaccaccac gattggttcc ttcccgcaaa
     6421 ccacggaaat tcgtaccctg cgtctggatt tcaaaaaggg caatctcgac gccaacaact
     6481 accgcacggg cattgcggaa catatcaagc aggccattgt tgagcaggaa cgtttgggac
     6541 tggatgtgct ggtacatggc gaggccgagc gtaatgacat ggtggaatac tttggcgagc
     6601 acctcgacgg atttgtcttt acgcaaaacg gttgggtaca gagctacggt tcccgctgcg
     6661 tgaagccacc gattgtcatt ggtgacatta gccgcccggc accgattacc gtggagtggg
     6721 cgaagtatgc gcaatcgctg accgacaaac cggtgaaagg gatgctgacg gggccggtga
     6781 ccatactctg ctggtcgttc ccgcgtgaag atgtcagccg tgaaaccatc gccaaacaga
     6841 ttgcgctggc gctgcgtgat gaagtggccg atctggaagc cgctggaatt ggcatcatcc
     6901 agattgacga accggcgctg cgcgaaggtt taccgctgcg tcgtagcgac tgggatgcgt
     6961 atctccagtg gggcgtagag gccttccgta tcaacgccgc cgtggcgaaa gatgacacac
     7021 aaatccacac tcacatgtgt tattgcgagt tcaacgacat catggattcg attgcggcgc
     7081 tggacgcaga cgtcatcacc atcgaaacct cgcgttccga catggagttg ctggagtcgt
     7141 ttgaagagtt tgattatcca aatgaaatcg gtcctggcgt ctatgacatt cactcgccaa
     7201 acgtaccgag cgtggaatgg attgaagcct tgctgaagaa agcggcaaaa cgcattccgg
     7261 cagagcgcct gtgggtcaac ccggactgtg gcctgaaaac gcgcggctgg ccagaaaccc
     7321 gcgcggcact ggcgaacatg gtgcaggcgg cgcagaattt gcgtcggggg taaatccaaa
     7381 ccgggtggta ataccacccg gtcttttctc attacagcga cttcttccca ccatactgct
     7441 taaaccattc cagcatacgc tgccagccat ctttcgcaga ttcggcatga tagctcgggc
     7501 gataatcagc gttgaatgca tgcccggcat ccgggtacac gataatctct gctttcgcat
     7561 tagcagcccg cagcgcctgg cgcatcgttt caacgctctc ctgcggaatg ctgttatcct
     7621 gaccaccata taagccgaga accggcgcgt taagatcggt tgcgatatca acaggttgtt
     7681 tcggtgaatt cagcgacttg tcgcccgtca gtttgccgta ccacgccact gcggctttta
     7741 gctgtggatt atgcgcggca tacagccagg tgatacgtcc accccagcag aatccggtga
     7801 tcattaaacg atgaacatcg ccgccgttgc gggaagccca actggcgaca tgatcgagat
     7861 cggccagcac ctgcgagtca ggcacttttg ctaccagacc gctaagcaac gtggggatat
     7921 cggcaaaatc attcggatcg ccttcgcgga agtaaagttc aggtgcgata gccagatacc
     7981 cctccagcgc cagacggcga caaatgtcgc ggatatgttc atgcacgcca aaaatttcct
     8041 gcactacaat gaccactggc agtgggccat cgctttgctt tggtctggca tggtaagcag
     8101 gcatgttatc cccttgtgaa gggatagagg tgaagcccgc cacaatcgcg tcgtccgggg
     8161 tctgaacgat ggtcgaagcg agaggcgatg cagcaggtgc aaatccagat tgttgtgttg
     8221 ttgccatggt attctccgta cccttataaa aatgttgcgc aatgttaact atagtcagca
     8281 tgcaacaaat cacattgcct gaatcggctc atcttttatg cagtcctgca gaatgaagag
     8341 tgatttatgt gatatgaatc acttttggtg ggtaaattta tgcaacgcat ttgcgtcatg
     8401 gtgatgagta tcacgaaaaa atgttaaacc cttcggtaaa gtgtcttttt gcttcttctg
     8461 actaaaccga ttcacagagg agttgtatat gtccaagtct gatgtttttc atctcggcct
     8521 cactaaaaac gatttacaag gggctacgct tgccatcgtc cctggcgacc cggatcgtgt
     8581 ggaaaagatc gccgcgctga tggataagcc ggttaagctg gcatctcacc gcgaattcac
     8641 tacctggcgt gcagagctgg atggtaaacc tgttatcgtc tgctctaccg gtatcggcgg
     8701 cccgtctacc tctattgctg ttgaagagct ggcacagctg ggcattcgca ccttcctgcg
     8761 tatcggtaca acgggcgcta ttcagccgca tattaatgtg ggtgatgtcc tggttaccac
     8821 ggcgtctgtc cgtctggatg gcgcgagcct gcacttcgca ccgctggaat tcccggctgt
     8881 cgctgatttc gaatgtacga ctgcgctggt tgaagctgcg aaatctattg gcgcgacaac
     8941 tcacgttggc gtgacagttt cttctgatac cttctaccca ggtcaggaac gttacgatac
     9001 ttactctggt cgcgtagttc gtcactttaa aggttctatg gaagagtggc aggcgatggg
     9061 cgtaatgaac tatgaaatgg aatctgcaac tctgctgacc atgtgtgcaa gccagggcct
     9121 gcgtgccggt atggtagcgg gtgttatcgt taaccgcacc cagcaagaga tcccgaatgc
     9181 tgagacgatg aaacaaactg aaagccatgc ggtgaaaatc gtggtggaag cggcgcgtcg
     9241 tctgctgtaa ttctcttctc ctgtctgaag gccgacgcgt tcggcctttt gtatttttgc
     9301 gtagcgcctc gcaggaaatg cctttccaac tggacgtttg tacagcacaa ttctattttg
     9361 tgcgggtaag ttgttgcgtc aggaggcgtt gtggatttct caatcatggt ttacgcagtt
     9421 attgcgttgg tgggtgtggc aattggctgg ctgtttgcca gttatcaaca tgcgcagcaa
     9481 aaagccgagc aattagctga acgtgaagag atggtcgcgg agttaagcgc ggcaaaacaa
     9541 caaattaccc aaagcgagca ctggcgtgca gagtgtgaat tgctcaataa cgaagtgcgc
     9601 agcctgcaaa gtattaacac ctctctggag gccgatctgc gtgaagtaac cacgcggatg
     9661 gaagccgcac agcaacatgc tgacgataaa attcgccaga tgattaacag cgagcagcgc
     9721 ctcagtgagc agtttgaaaa cctcgccaac cgtatttttg agcacagcaa tcgccgggtt
     9781 gatgagcaaa accgtcagag tctgaacagc ctgttgtcgc cgctacgtga acaactggac
     9841 ggtttccgcc gtcaggttca ggacagcttc ggtaaagaag cacaagaacg ccataccctg
     9901 acccacgaaa ttcgcaatct ccagcaactc aacgcgcaaa tggcccagga agcgatcaac
     9961 ctgacgcgcg cgctgaaagg cgacaataaa acccagggca actggggcga ggtagtattg
    10021 acgcgggtgc tggaggcttc cggtctgcgt gaagggtatg aatatgaaac ccaggtcagc
    10081 atcgaaaatg acgcccgctc gcggatgcag ccggatgtca tcgtgcggct gccgcaggga
    10141 aaagatgtgg tgatcgacgc caaaatgacg ctggtcgcct atgaacgcta ttttaacgcc
    10201 gaagacgact acacccgcga aagcgcgcta caggaacata tcgcgtcggt gcgtaaccat
    10261 atccgtttgc tgggacgcaa agattatcaa cagctgccgg ggctgcgaac tctggattac
    10321 gtgctgatgt ttattcccgt tgaacccgct tttttactgg cgcttgaccg ccagccggag
    10381 ctgatcaccg aagcgttgaa aaacaatatc atgctggtta gcccgactac gctgctggtg
    10441 gcgttgcgta ctattgccaa cctgtggcgt tatgagcatc aaagccgtaa cgcccagcaa
    10501 atcgccgatc gtgccagcaa gctgtacgac aagatgcgtt tgttcatcga tgacatgtcc
    10561 gcgattggtc aaagtctcga caaagcgcag gataactacc gccaggcaat gaaaaaactc
    10621 tcttcaggtc gcggaaatgt gctggcgcag gcagaagcgt ttcgcggttt aggagtagaa
    10681 attaaacgcg agattaatcc ggatttggct gaacaggcgg tgagccagga tgaagagtat
    10741 cgacttcggt cggttccggg gcagccgaat gatgaagctt atcaacgcga tgatgaatat
    10801 aatcagcagt cgcgctagcc cattaggggt agttaagccg ggtagaaatc tagggcatcg
    10861 acgcccaatc tgttacactt ctggaacaat tttttgatga gcaggcattg agatggtgga
    10921 taagtcacaa gaaacgacgc actttggttt tcagaccgtc gcgaaggaac aaaaagcgga
    10981 tatggtcgcc cacgttttcc attccgtggc atcaaaatac gatgtcatga atgatttgat
    11041 gtcatttggt attcatcgtt tgtggaagcg attcacgatt gattgcagcg gcgtacgccg
    11101 tgggcagacc gtgctggatc tggctggtgg caccggcgac ctgacagcga aattctcccg
    11161 cctggtcgga gaaactggca aagtggtcct tgctgatatc aatgaatcca tgctcaaaat
    11221 gggacgcgag aagctgcgta atatcggtgt gattggcaac gttgagtatg ttcaggcgaa
    11281 cgctgaggcg ctgccgttcc cggataacac ctttgattgc atcaccattt cgtttggtct
    11341 gcgtaacgtc accgacaaag ataaagcact gcgttcaatg tatcgcgtgc tgaaacccgg
    11401 cggccgcctg ctggtgcttg agttctcgaa gccaattatc gagccgctga gcaaagccta
    11461 tgatgcatac tccttccatg tgctgccgcg tattggctca ctggtcgcga acgacgccga
    11521 cagctaccgt tatctggcag aatccatccg tatgcatccc gatcaggata ccctgaaagc
    11581 catgatgcag gatgccggat tcgaaagtgt cgactactac aatctgacgg caggggttgt
    11641 ggcgctgcat cgtggttata agttctgaca ggagaccgga aatgcctttt aaacctttag
    11701 tgacggcagg aattgaaagt ctgctcaaca ccttcctgta tcgctcaccc gcgctgaaaa
    11761 cagcccgctc gcgtctgctg ggtaaagtat tgcgcgtgga ggtaaaaggc ttttcgacgt
    11821 cattgattct ggtgttcagc gaacgccagg ttgatgtact gggcgaatgg gcaggcgatg
    11881 ctgactgcac cgttatcgcc tacgccagtg tgttgccgaa acttcgcgat cgccagcagc
    11941 ttaccgcact gattcgcagt ggtgagctgg aagtgcaggg cgatattcag gtggtgcaaa
    12001 acttcgttgc gctggcagat ctggcagagt tcgaccctgc ggaactgctg gccccttata
    12061 ccggtgatat cgccgctgaa ggaatcagca aagccatgcg cggaggcgca aagttcctgc
    12121 atcacggcat taagcgccag caacgttatg tggcggaagc cattactgaa gagtggcgta
    12181 tggcacccgg tccgcttgaa gtggcctggt ttgcggaaga gacggctgcc gtcgagcgtg
    12241 ctgttgatgc cctgaccaaa cggctggaaa aactggaggc taaatgacgc ctggtgaagt
    12301 acggcgccta tatttcatca ttcgcacttt tttaagctac ggacttgatg aactgatccc
    12361 caaaatgcgt atcaccctgc cgctacggct atggcgatac tcattattct ggatgccaaa
    12421 tcggcataaa gacaaacttt taggtgagcg actacgactg gccctgcaag aactggggcc
    12481 ggtttggatc aagttcgggc aaatgttatc aacccgccgc gatctttttc caccgcatat
    12541 tgccgatcag ctggcgttat tgcaggacaa agttgctccg tttgatggca agctggcgaa
    12601 gcagcagatt gaagctgcaa tgggcggctt gccggtagaa gcgtggtttg acgattttga
    12661 aatcaagccg ctggcttctg cttctatcgc ccaggttcat accgcgcgat tgaaatcgaa
    12721 tggtaaagag gtggtgatta aagtcatccg cccggatatt ttgccggtca ttaaagcgga
    12781 tctgaaactt atctaccgtc tggctcgctg ggtgccgcgt ttgctgccgg atggtcgccg
    12841 tctgcgccca accgaagtgg tgcgcgagta cgaaaagacc ttgattgatg aactgaattt
    12901 gctgcgggaa tctgccaacg ccattcagct tcggcgcaat tttgaagaca gcccgatgct
    12961 ctacatcccg gaagtttacc ctgactattg tagtgaaggg atgatggtga tggagcgcat
    13021 ttacggcatt ccggtgtctg atgttgcggc gctggagaaa aacggcacta acatgaaatt
    13081 gctggcggaa cgcggcgtgc aggtgttctt cactcaggtc tttcgcgaca gctttttcca
    13141 tgccgatatg caccctggca acatcttcgt aagctatgaa cacccggaaa acccgaaata
    13201 tatcggcatt gattgcggga ttgttggctc gctaaacaaa gaagataaac gctatctggc
    13261 agaaaacttt atcgccttct ttaatcgcga ctatcgcaaa gtggcagagc tacacgtcga
    13321 ttctggctgg gtgccaccag ataccaacgt tgaagagttc gaatttgcca ttcgtacggt
    13381 ctgtgaacct atctttgaga aaccgctggc cgaaatttcg tttggacatg tactgttaaa
    13441 tctgtttaat acggcgcgtc gcttcaatat ggaagtgcag ccgcaactgg tgttactcca
    13501 gaaaaccctg ctctatgtcg aaggggtagg acgccagctt tatccgcagc tcgatttatg
    13561 gaaaacagcg aagcctttcc tggagtcgtg gattaaagat caggtcggta ttcctgccct
    13621 ggtgagagca tttaaagaaa aagcgccgtt ctgggtcgaa aaaatgccag aactgcctga
    13681 actggtttac gacagtttgc ggcagggcaa gtatttacag cacagtgttg ataagattgc
    13741 ccgcgagctt cagtcaaatc atgtacgtca gggacaatcg cgttattttc tcggaattgg
    13801 cgctacgtta gtattaagtg gcacattctt gttggtcagc cgacctgagt gggggctgat
    13861 gcccggctgg ttaatggcag gtggtctgat cgcctggttt gtcggttggc gcaaaacacg
    13921 ctgatttttt catcgctcaa ggcgggccgt gtaacgtata atgcggcttt gtttaatcat
    13981 catctaccac agaggaacat gtatgggtgg tatcagtatt tggcagttat tgattattgc
    14041 tgtcatcgtt gtactgcttt ttggcaccaa aaagctcggc tccatcggtt ccgatcttgg
    14101 tgcgtcgatc aaaggcttta aaaaagcaat gagcgatgat gaaccaaagc aggataaaac
    14161 cagtcaggat gctgatttta ctgcgaaaac tatcgccgat aagcaggcgg atacgaatca
    14221 ggaacaggct aaaacagaag acgcgaagcg ccacgataaa gagcaggtgt aatccgtgtt
    14281 tgatatcggt tttagcgaac tgctattggt gttcatcatc ggcctcgtcg ttctggggcc
    14341 gcaacgactg cctgtggcgg taaaaacggt agcgggctgg attcgcgcgt tgcgttcact
    14401 ggcgacaacg gtgcagaacg aactgaccca ggagttaaaa ctccaggagt ttcaggacag
    14461 tctgaaaaag gttgaaaagg cgagcctcac taacctgacg cccgaactga aagcgtcgat
    14521 ggatgaacta cgccaggccg cggagtcgat gaagcgttcc tacgttgcaa acgatcctga
    14581 aaaggcgagc gatgaagcgc acaccatcca taacccggtg gtgaaagata atgaagctgc
    14641 gcatgagggc gtaacgcctg ccgctgcaca aacgcaggcc agttcgccgg aacagaagcc
    14701 agaaaccacg ccagagccgg tggtaaaacc tgctgcggac gcggaaccga aaaccgctgc
    14761 accttcccct tcgtcgagtg ataaaccgta aacatgtctg tagaagatac tcaaccgctt
    14821 atcacgcatc tgattgagct gcgtaagcgt ctgctgaact gcattatcgc ggtgatcgtg
    14881 atattcctgt gtctggtcta tttcgccaat gacatctatc acctggtatc cgcgccattg
    14941 atcaagcagt tgccgcaagg ttcaacgatg atcgccaccg acgtggcctc gccgttcttt
    15001 acgccgatca agctgacctt tatggtgtcg ctgattctgt cagcgccggt gattctctat
    15061 caggtgtggg catttatcgc cccagcgctg tataagcatg aacgtcgcct ggtggtgccg
    15121 ctgctggttt ccagctctct gctgttttat atcggcatgg cattcgccta ctttgtggtc
    15181 tttccgctgg catttggctt ccttgccaat accgcgccgg aaggggtgca ggtatccacc
    15241 gacatcgcca gctatttaag cttcgttatg gcgctgttta tggcgtttgg tgtctccttt
    15301 gaagtgccgg tggcaattgt gctgctgtgc tggatgggga ttacctcgcc agaagactta
    15361 cgcaaaaaac gcccgtatgt gctggttggt gcattcgttg tcgggatgtt gctgacgccg
    15421 ccggatgtct tctcgcaaac gctattggcg atcccgatgt actgtctgtt tgaaatcggt
    15481 gtcttcttct cacgctttta cgttggtaaa gggcgaaatc gggaagagga aaacgacgct
    15541 gaagcagaaa gcgaaaaaac tgaagaataa attcaaccgc ccgtcagggc ggttgtcata
    15601 tggagtacag gatgtttgat atcggcgtta atttgaccag ttcgcaattt gcgaaagacc
    15661 gtgatgatgt tgtagcgcgc gcttttgacg cgggagttaa tgggctactc atcaccggca
    15721 ctaacctgcg tgaaagccag caggcgcaaa agctggcgcg tcagtattcg tcctgttggt
    15781 caacggcggg cgtacatcct cacgacagca gccagtggca agctgcgact gaagaagcga
    15841 ttattgagct ggctgcgcag ccagaagtgg tggcgattgg tgaatgtggt ctcgacttta
    15901 accgcaactt ttcgacgccg gaagagcagg aacgcgcttt tgttgctcag ctacgcattg
    15961 ccgcagaatt aaacatgccg gtatttatgc actgtcgcga tgcccacgag cggtttatga
    16021 cattgctgga gccgtggctg gataaactgc ctggtgcggt tcttcattgc tttaccggca
    16081 cacgcgaaga gatgcaggcg tgcgtggcgc gtggaattta tatcggcatt accggttggg
    16141 tttgcgatga acgacgcggg ctggagctgc gggaattgtt gccgttgatt ccggcggaga
    16201 aattgctgat cgaaacagat gcgccgtatc tgctcccgcg cgatctcacg ccaaagccat
    16261 catcccggcg caacgagcca gcccatctgc cccatatttt gcaacgtatt gcgcactggc
    16321 gtggagaaga tgccgcatgg ctggctgcca ccacggatgc caatgtcaaa acactgtttg
    16381 ggattgcgtt ttagagtttg cggaactcgg tgttcttcac actgtgctta atctctttat
    16441 taataagatt aagcaatagc atggagcgag cctcaccatc gggttcggtg aaaatggcct
    16501 gaaagccttc gaacgcgcct tcggtaataa tcaccttatc acccggataa gggggggccg
    16561 gatcgacaat gtctttcggt ttatataccg atagctgatg aatcaccgcc gatgggacta
    16621 tcgctggcga cgcgccaaag cgcacaaagt ggctgacgcc acgcgtagcg ctgatggtcg
    16681 tggtatgaat cacttctgga tcaaattcca caaacaggta gttagggaat aacggttcac
    16741 tgactgcggt acgtttaccc cgtacgattt tttccagggt gatcatcgga gcgaggcaat
    16801 taaccgcctg tctctcaagg tgttcctggg cacgttgaag ttgcccgcgc ttgcagtaca
    16861 gtaaatacca ggattgcata atgactctta tccgtttaat cggggcgcaa ggatagcaaa
    16921 agctttacgc taagttaatt atattccccg gtttgcgtta taccgtcaga gttcacgcta
    16981 atttaacaaa tttacagcat tgcaaagatg aacgccgtat aatgggcgca gattaagagg
    17041 ctacaatgga cgccatgaaa tataacgatt tacgcgactt cctgacgctg cttgaacagc
    17101 agggtgagct aaaacgtatc acgctcccgg tggatccgca tctggaaatc actgaaattg
    17161 ctgaccgcac tctgcgtgct ggtgggcctg cgctgttgtt cgaaaaccct aaaggctact
    17221 caatgccggt gctgtgcaac ctgttcggta cgccaaagcg cgtggcgatg ggcatggggc
    17281 aggaagatgt ttcggcgctg cgtgaagttg gtaaattatt ggcgtttctg aaagagccgg
    17341 agccgccaaa aggtttccgc gacctgtttg ataaactgcc gcagtttaag caggtgttaa
    17401 acatgccgac aaagcgactg cgtggtgcgc cctgccaaca aaaaatcgtc tctggcgatg
    17461 acgtcgatct caatcgcatt cccattatga cctgctggcc ggaagatgcc gcgccgctga
    17521 ttacctgggg gctgaccgta acgcgcggcc cgcataaaga gcggcagaat ctgggcattt
    17581 atcgccagca gctgattggt aaaaacaaac tgattatgcg ctggctgtcg catcgcggcg
    17641 gcgcgctgga ttatcaggag tggtgtgcgg cgcatccggg cgaacgtttc ccggtttctg
    17701 tggcgctggg tgccgatcct gccacgattc tcggtgcagt cacccccgtt ccggatacgc
    17761 tttcagagta tgcgtttgcc ggattgctac gcggcaccaa aaccgaagta gtaaagtgta
    17821 tttccaatga cctcgaagtg ccagccagtg ccgaaatcgt cctggaaggg tatatcgatc
    17881 ctggtgagat ggcgccggaa gggccgtatg gtgaccacac aggttactat aatgaagtcg
    17941 atagtttccc ggtgtttacc gtgacgcata ttacccagcg tgaagatgcg atttaccatt
    18001 ccacctatac cgggcgtccg ccagatgagc ccgcggtact gggcgtggcg ttgaacgaag
    18061 tgtttgtacc gattctgcaa aaacagttcc cggaaattgt cgatttttac ctgccgccgg
    18121 aaggctgttc ttatcgtctg gcggtagtga cgatcaaaaa acagtacgcc ggacacgcga
    18181 agcgcgtcat gatgggcgtc tggtcgttct tacgccagtt tatgtacact aaatttgtga
    18241 tcgtttgcga tgatgacgtc aacgcacgcg actggaacga tgtgatttgg gcgattacca
    18301 cccgtatgga cccggcgcgg gatactgttc tggtagaaaa tacgcctatt gattatctgg
    18361 attttgcctc gcctgtctct gggctgggtt caaaaatggg gctggatgcc acgaataaat
    18421 ggccggggga aacccagcgt gaatggggac gtcccatcaa aaaagatcca gatgttgtca
    18481 cgtatattga cgccatctgg gatgaactgg ctatttttaa caacggtaaa agcgcctgat
    18541 gcgcgtttgt tttgccctat ttatcgatcc gacagagaaa gcgcatgaca accttaagct
    18601 gtaaagtgac ctcggtagaa gctatcacgg ataccgtata tcgtgtccgc atcgtgccag
    18661 acgcggcctt ttcttttcgt gctggtcagt atttgatggt agtgatggat gagcgcgaca
    18721 aacgtccgtt ctcaatggcc tcgacgccgg atgaaaaagg gtttatcgag ctgcatattg
    18781 gcgcttctga aatcaacctt tacgcgaaag cagtcatgga ccgcatcctc aaagatcatc
    18841 aaatcgtggt cgacattccc cacggagaag cgtggctgcg cgatgatgaa gagcgtccga
    18901 tgattttgat tgcgggcggc accgggttct cttatgcccg ctcgattttg ctgacagcgt
    18961 tggcgcgtaa cccaaaccgt gatatcacca tttactgggg cgggcgtgaa gagcagcatc
    19021 tgtatgatct ctgcgagctt gaggcgcttt cgttaaagca ccctggtctg caagtggtgc
    19081 cggtggttga acaaccggaa gcaggctggc gtgggcgtac tggtaccgtg ttaacggcgg
    19141 tattgcagga tcatggtacg ctggcagagc atgatatcta tattgccgga cgttttgaga
    19201 tggcgaaaat tgcccgtgac ctgttttgca gtgagcgtaa tgcgcgggaa gatcgcctgt
    19261 ttggcgatgc gtttgcattt atctgagata taaaaaaacc cgcccctgac aggcgggaag
    19321 aacggcaact aaaactttat tcagtggcat ttagatctat gtcgcatccg gcaagtggtt
    19381 aaacccgctc aaacaccgtc gcaataccct gacccagacc gatacacatc gtcgccagac
    19441 caaactgaac gtctttgcgt tccatcagat tcagcagcgt ggtgctgata cgcgcaccgg
    19501 aacaacccag cggatgaccc agcgcgatcg caccaccatt gaggttgatc ttctcgtcaa
    19561 tctgctccat tagcccgaga tctttaatgc acggcaggat ctgcgcggca aaggcttcgt
    19621 tcatctcaaa cacgccgata tcgctggcag aaagccccgc ttttttcagc gccagcttcg
    19681 aggccggaac cgggccgtaa cccataatcg atgggtcaca accaacgacc gccatcgaac
    19741 gcacacgagc gcgcggctta agacctaatt catgggcgcg gctttcactc atcaccagca
    19801 tggcagctgc gccatcggaa agtgcagaag atgtgcccgc cgttaccgta ccgtttactg
    19861 gatcaaacgc cggacgcagc gtggcgaggg cttccacggt ggtttccggg cgaatcactt
    19921 cgtcgtaatt aaactgcttc aggacgccgt cggcatcgtg accaccagtt gggatgattt
    19981 catttttaaa tgcggccgac tgcgtggcgg cccaggcgcg ggcgtgtgac cgcgcggcaa
    20041 aggcatcctg catttcacgg ctgataccgt gtatacgcgc cagcatttct gccgttaagc
    20101 ccatcatgcc cgccgctttg gcgacattgc ggctcaggcc ggggtgaaaa tcgacgccgt
    20161 gactcatcgg cacatggccc atatgctcca cgccgccaac cagacatgcc tgcgcatcgc
    20221 cagtcatgat cattcgtgct gcgtcatgca gtgcctgcat ggatgaacca cacaagcgat
    20281 tgacggtaac cgccgggaca gagtgcggca cttctgccag cagcgccgcg ttacgggcga
    20341 tgttaaaacc ctgctccagc gtctgttgca cacaacccca gtaaatatcg tcgagggccg
    20401 ccgcttccag cgccgggtta cgcgccagca ggctacgcat taaatgagcg gagagatctt
    20461 ccgcacgcac gttacgaaaa gcaccgccct tcgaacggcc catcggggtg cgaattgcat
    20521 cgacaatgac aacctgttcc attgtgattc cttaagccgt tttcaggtcg ccaaccggac
    20581 gggctggctc aaccggagga tagtacggtt cgttatgacg cgctttatta cgcagacctt
    20641 ccggcacttc atacagcggg ccgaggtgct gatattgctg tgccatatcg aggtattttg
    20701 cgctaccgag ggtgtccagc cagcggaacg cgccgccgtg gaacggaggg aagcccaggc
    20761 cgtagaccag cgccatatcc gcttccgccg gagtggcgat aatgccctct tccagacagc
    20821 gcaccacttc gttgaccatc gggatcatca tgcgggcgat aatctcttct tcgctgaaat
    20881 cgcgcttcgg ttggctcact tctgccagca ggtcttcaac ggcggcgtct tcttctttct
    20941 tcggcttacc tttgctgtct tctttataac gccagaaacc gaggccgttc ttctgaccaa
    21001 agcggttggc atcaaacagc gcgtcgatgg catcgcggta atctttctgc atccgctgcg
    21061 ggaagcctgc tgccatgaca gcctgagcgt gatgcgcggt atcaatgccc acaacgtcca
    21121 gcagatatgc cgggcccatc ggccagccaa actgtttttc catcactttg tcgatcttgc
    21181 ggaaatccgc gccgtcgcgc agcagctggc tgaaaccggc gaaatacggg aacagcacgc
    21241 ggttaacaaa gaagccgggg cagtcgttaa ccacaatcgg cgtcttgccc atcttgctcg
    21301 cccaggcgac aactttcgcg atggtttcgt cggagctttt ctcgccgcga ataatttcta
    21361 ccaacggcat tcggtggacc gggttaaaga agtgcatccc gcagaagttt tccgggcgtt
    21421 ccagcgcgtt ggccagttcg ctgataggaa tggttgaagt gttagacgcc agcacggtat
    21481 cctggcgtac tttttgttcg gtttctgcca gtacggcttt tttcactttc gggttttcaa
    21541 caaccgcttc taccacaata tccacgcggt caaatccggc gtagtcgagc gttgggtgga
    21601 tcgtggagat cacgccagcc agtttcagac catcgatctt gccgcgctca agctgcttgt
    21661 tcagcagttt cgcggcttcg gtcatgccga gggttaacga cttgtcgttg atatctttca
    21721 tgacaaccgg cacgcctttc cacgcggact ggtaagcgat accgccaccc atgatgcctg
    21781 cgcccagcac agcagcctgt ttcggggttt caacgtcttt ggtgagtttc ttcgctttgc
    21841 cttttacata ttgatcgtta aggaaaatgc cgaccagtgc gcgagcttcg ttggtatgcg
    21901 ccagcgggac aaaacttttg ttttccagat ttaaggcttc ttcacgacca aaacgggccg
    21961 cagcttcaat ggtttttact gcggtgatgg gggccggata atgtttcccc gccgtttgcg
    22021 cgaccattcc tttagcgatg gtgaagctca tggtggcttc aatcttgctc agttttagcg
    22081 gttccagctt cggctgacgt ttcgctttcc agtcgaggtc gccgttaatg gcctggcgta
    22141 aaatcgccat cgccccttca atcagttttt ctgctttgac tacgccatcc accagaccga
    22201 ttttcagcgc ctgatccgcg ccgacatctt taccggcggc agtgatttcc agtgcactgt
    22261 cagcgcccag catacgtggc atacgtacag aaccgccaaa gcccggcatg atgcccagtt
    22321 tggtttccgg cagaccgatg cgcagatccg gcgtcgccag acgataatcg gtcgccagca
    22381 cgcattcgca gccaccaccc agcgcatagc cgttgacggc agcaatggtc ggcaccggca
    22441 gatcttccag gcgattaaac acgctattgg caaagtgcag ccactgactt aactgttctt
    22501 caggaacgag gaacagggac aaaaattcgg tgatatcagc accgacgata aaggctgctt
    22561 tgttcgaacg cagcagcagc ccttttagat ctgattgctg ttccagcacg ccgatggcct
    22621 cgccgaggct ggcgacggtc gcagtgtcga gtttattaac tgaacctggg gcatcaaata
    22681 ccagttcggc aatgccatct tccagccagt caaggtacag ggtgtcgcct ttgtaaagca
    22741 tgtcagtctc ctgaatccgc aaggtgatct ggtcgtacca gatgcatcgg agtgtgtgtt
    22801 ttgtgttaaa aatatgcaaa taaaagatta aagaaatgcc gatctgatca cgctcggcag
    22861 aaatcacgct ctggatgaac gatgtgctaa gatgcggaga cttaaggtca aaaaaacaga
    22921 agggtaaaaa atggaatcac tggcctcgct ctataaaaat catatagcta ccttacaaga
    22981 acggactcgc gatgcgctgg cgcgcttcaa gctggatgca ttacttattc actccggcga
    23041 gctgttcaac gtttttctcg acgatcatcc ctatccgttt aaagtgaacc cgcaattcaa
    23101 agcgtgggtg ccggtaactc aggtgccaaa ctgctggttg ctggtggatg gcgtgaacaa
    23161 gccgaaactg tggttctatc tgccggttga ttactggcac aacgtcgaac cgctgccgac
    23221 ctccttctgg actgaagatg tggaagtgat cacgctgccg aaagccgatg gcattggtag
    23281 cctgctgcct gctgcgcgcg gcaatatcgg ttatatcggt ccggtgccgg aacgtgcgct
    23341 gcaactgggt attgaggcca gcaacatcaa cccgaaaggg gtgatcgact acctgcatta
    23401 ttaccgctcc ttcaaaaccg agtacgaact ggcctgtatg cgtgaagcgc agaaaatggc
    23461 ggtcaatggt catcgtgcgg cagaagaagc gttccgttct ggcatgagcg agttcgatat
    23521 caatattgcc tatctgaccg cgaccggtca tcgtgatacc gacgtacctt acagcaacat
    23581 tgtggcgctt aacgaacacg ctgcggtgtt gcattacacc aaactggacc accaggcacc
    23641 ggaagagatg cgcagcttcc tgctggatgc cggggccgaa tataacggct atgcggctga
    23701 cctgacccgt acctggtcgg caaaaagcga caacgactac gcacagctgg tgaaagacgt
    23761 aaatgatgaa caactggggc tgatcgccac catgaaagca ggcgtcagct atgtggatta
    23821 ccacatccag ttccatcagc gcatcgccaa actgctgcgt aaacatcaaa tcatcaccga
    23881 tatgagtgaa gaagcgatgg tcgaaaacga tctcaccgga ccgtttatgc cgcacggtat
    23941 tggccatccg ctgggcctgc aggtgcatga cgtcgccggt tttatgcagg atgatagcgg
    24001 tacacacctc gcggcaccgg caaaatatcc gtacctgcgc tgcacccgta ttctccagcc
    24061 gggcatggtg ttaaccatcg aaccgggtat ctacttcatt gaatcgcttc tggcaccgtg
    24121 gcgtgaaggg cagttcagca agcacttcaa ctggcagaaa attgaagcac tgaaaccgtt
    24181 cggcggcatt cgtatcgaag acaacgtggt gatccacgaa aacaacgtgg aaaacatgac
    24241 ccgggatctg aaactggcgt gatggaaagc tggttaattc ctgcggcacc ggtcacggtc
    24301 gttgaagaga tcaaaaagag ccgcttcata acgatgttgg cgcataccga tggcgttgag
    24361 gcggcgaaag cgtttgttga atcggtgcgg gcagaacacc ccgatgcccg ccaccattgc
    24421 gtggcgtggg tggcgggtgc gccggatgat tctcaacagc tgggtttctc tgacgacggg
    24481 gagccggcgg gaacggcagg taaaccgatg ctcgcccagc taatgggcag cggcgtcggg
    24541 gaaattaccg ctgtggtagt gcgctactac ggcggcatat tgcttggcac cggtgggtta
    24601 gtgaaagcgt atggcggcgg cgtgaatcag gcgctgcgcc agctaacgac ccaacgcaag
    24661 acgccattaa ccgaatatac tttgcaatgt gaatatagtc agttaaccgg cattgaagcg
    24721 ttgctggggc agtgtgacgg caaaattatc aacagtgatt atcaggcatt cgttctgctg
    24781 cgggtggcgc ttccggcggc gaaagtggct gaattttccg caaagctggc ggattttagc
    24841 cgtggttcat tgcaattgtt agcgattgaa gaataatccc cacttcgttt tgcagaacta
    24901 aggaagcggc agagatgcat tttcgcgcca ttacccgaat cgttggacta ctggtcatct
    24961 tattttcagg gaccatgatt atccctgggc tggtagcact catctaccgg gatggagcgg
    25021 gccgcgcttt tacccagacc ttttttgtcg ccctcgccat tggctctatg ctgtggtggc
    25081 cgaaccgcaa agagaagggc gaactgaaat cccgtgaggg gtttctgata gtggtgctgt
    25141 tttggaccgt gctgggtagt gtcggtgcgc tcccttttat cttctcggaa agcccgaacc
    25201 tcacgattac cgatgcgttt tttgaatctt tctctggcct gaccaccacc ggggccacta
    25261 cgctggtggg gctggattcg ctccctcatg ccatcctctt ttatcgccag atgctgcaat
    25321 ggtttggcgg gatggggatc atcgtgttag cggttgcgat actgcctatc ctcggcgtgg
    25381 gtgggatgca gctctatcgc gcagaaatgc ccggcccgct gaaagataac aaaatgcgcc
    25441 cgcgaattgc ggaaacggcg aaaactctgt ggttgattta tgtcttgctg accgtcgcct
    25501 gtgcgctggc gttgtggttt gccggaatgg atgcctttga tgccatcggc catagctttg
    25561 cgactatcgc tattggcggc ttctcgacac atgatgccag tatcggttat ttcgacagtc
    25621 cggctattaa cactatcatt gctatcttcc tgctgatctc cggctgtaac tacggtctgc
    25681 acttttcact gttaagtggg cgtagtctga aggtttattg gcgcgatccg gaatttcgca
    25741 tgtttatcgg cgtacagttt acgctggtgg ttatttgtac actcgtactg tggtttcata
    25801 atgtctacag ttcggcgctg atgacaatta accaggcgtt tttccaggtg gtatcgatgg
    25861 cgacaaccgc cgggtttacg actgacagca ttgcccgctg gccgctcttt ttgccggtac
    25921 tgcttttatg ttcagctttt atcggcggtt gcgccgggtc aacgggcggt ggcctgaaag
    25981 tgatccgcat cctgctgctg tttaagcagg ggaaccgtga gctgaaacga ctggtgcatc
    26041 cgaacgccgt ctatagcatt aagctgggga atcgcgcact gccggaacgt atcctcgaag
    26101 ccgtttgggg atttttctcc gcctatgcat tggtgtttat tgtcagtatg ctggcgatta
    26161 tcgccacggg cgtggatgat ttttctgcct ttgcctcagt tgttgcgaca ttgaataacc
    26221 tggggccagg gcttggcgtg gttgctgata actttaccag tatgaacccg gtggctaaat
    26281 ggatcctgat tgccaacatg ctgtttggtc gtctcgaggt ctttacattg ctggtgctct
    26341 ttaccccgac tttctggcgt gaatgatgga gtaatacgtg aaaacattaa ttcttttctc
    26401 aacaagggac ggacaaacgc gcgagattgc ctcctacctg gcttcggaac tgaaagaact
    26461 ggggatccag acggatgtcg ccaatgtgca ccgcattgaa gaaccacaat gggaaaacta
    26521 tgaccgtgtg gtcattggtg cttctattcg ctatggtcac taccactctg cgttccagga
    26581 atttgtcaaa aaacatgcga tgcgactgaa ttcgatgccg agcgcctttt attccgtaaa
    26641 cctggtggcg cgcaaaccgg agaagcgtac tccacagacc aacagctacg cgcggaagtt
    26701 tctgatgaac tcgcaatggc gtcccgatcg ctgcgcggtc attgccgggg cgctgcgtta
    26761 cccacgttat cgctggtacg accgttttat gctcaagctg attatgaaga tgtcaggcgg
    26821 tgaaacggat acgcgcaaag aagttgtcta taccgattgg gagcaggtgg cgaatttcgc
    26881 ccgagaaatc gcccatttaa ccgacaaacc gacgctgaaa taagcataaa gaataaaaaa
    26941 tgcgcggtca gaaaattatt ttaaatttcc tcttgtcagg ccggaataac tccctataat
    27001 gcgccaccac tgacacggaa caacggcaaa cacgccgccg ggtcagcggg gttctcctga
    27061 gaattccgac agagaaagca aaaataaatg cttgactctg tagcgggaaa gcgtattatg
    27121 cacaccc
//
LOCUS       DADMEQ010000048        26412 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.048, whole genome shotgun sequence.
ACCESSION   DADMEQ010000048 DADMEQ010000000
VERSION     DADMEQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.048"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            117..1088
                     /locus_tag="JNZ05_002040"
     CDS             117..1088
                     /locus_tag="JNZ05_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY8565280.1"
                     /translation="MRTTRNLLLMATLLGSALFAMAADKEMTIGAIYLDTQGYYAGVR
                     QGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSSRTVR
                     RASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKIDQPKI
                     AVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIISTPDLN
                     AIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDISGKKM
                     GNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            1111..2622
                     /locus_tag="JNZ05_002041"
     CDS             1111..2622
                     /locus_tag="JNZ05_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY8565281.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2647..3645
                     /locus_tag="JNZ05_002042"
     CDS             2647..3645
                     /locus_tag="JNZ05_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8565282.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNAQPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3678..4772
                     /locus_tag="JNZ05_002043"
     CDS             3678..4772
                     /locus_tag="JNZ05_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAY8565283.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGAVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            4784..5656
                     /locus_tag="JNZ05_002044"
     CDS             4784..5656
                     /locus_tag="JNZ05_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAY8565284.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALALG
                     ESTTSEMSLRVEWL"
     gene            complement(5704..6126)
                     /locus_tag="JNZ05_002045"
     CDS             complement(5704..6126)
                     /locus_tag="JNZ05_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY8565285.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6223..7430)
                     /locus_tag="JNZ05_002046"
                     /pseudo
     CDS             complement(6223..7430)
                     /locus_tag="JNZ05_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            complement(7440..8252)
                     /gene="hcaB"
                     /locus_tag="JNZ05_002047"
     CDS             complement(7440..8252)
                     /gene="hcaB"
                     /locus_tag="JNZ05_002047"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(cis-5,6-dihydroxycyclohexa-1,
                     3-dien-1-yl)propanoate dehydrogenase"
                     /protein_id="HAY8565286.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVAALELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(8249..8569)
                     /locus_tag="JNZ05_002048"
     CDS             complement(8249..8569)
                     /locus_tag="JNZ05_002048"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HAY8565287.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(8569..9087)
                     /locus_tag="JNZ05_002049"
                     /pseudo
     CDS             complement(8569..9087)
                     /locus_tag="JNZ05_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
     gene            complement(9084..10445)
                     /locus_tag="JNZ05_002050"
     CDS             complement(9084..10445)
                     /locus_tag="JNZ05_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HAY8565288.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            10581..11471
                     /gene="hcaR"
                     /locus_tag="JNZ05_002051"
     CDS             10581..11471
                     /gene="hcaR"
                     /locus_tag="JNZ05_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HAY8565289.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            11631..12770
                     /locus_tag="JNZ05_002052"
     CDS             11631..12770
                     /locus_tag="JNZ05_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HAY8565290.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(12762..14042)
                     /gene="csiE"
                     /locus_tag="JNZ05_002053"
     CDS             complement(12762..14042)
                     /gene="csiE"
                     /locus_tag="JNZ05_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HAY8565291.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKHFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(14233..15087)
                     /locus_tag="JNZ05_002054"
     CDS             complement(14233..15087)
                     /locus_tag="JNZ05_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY8565292.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVLRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTSPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            complement(15232..16035)
                     /gene="suhB"
                     /locus_tag="JNZ05_002055"
     CDS             complement(15232..16035)
                     /gene="suhB"
                     /locus_tag="JNZ05_002055"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HAY8565293.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKR"
     gene            16154..16894
                     /gene="trmJ"
                     /locus_tag="JNZ05_002056"
     CDS             16154..16894
                     /gene="trmJ"
                     /locus_tag="JNZ05_002056"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HAY8565294.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            17164..17652
                     /gene="iscR"
                     /locus_tag="JNZ05_002057"
     CDS             17164..17652
                     /gene="iscR"
                     /locus_tag="JNZ05_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HAY8565295.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            17764..18978
                     /gene="iscS"
                     /locus_tag="JNZ05_002058"
     CDS             17764..18978
                     /gene="iscS"
                     /locus_tag="JNZ05_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAY8565296.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            19006..19392
                     /gene="iscU"
                     /locus_tag="JNZ05_002059"
     CDS             19006..19392
                     /gene="iscU"
                     /locus_tag="JNZ05_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HAY8565297.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            19409..19732
                     /gene="iscA"
                     /locus_tag="JNZ05_002060"
     CDS             19409..19732
                     /gene="iscA"
                     /locus_tag="JNZ05_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HAY8565298.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            19828..20343
                     /gene="hscB"
                     /locus_tag="JNZ05_002061"
     CDS             19828..20343
                     /gene="hscB"
                     /locus_tag="JNZ05_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HAY8565299.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            20360..22210
                     /gene="hscA"
                     /locus_tag="JNZ05_002062"
     CDS             20360..22210
                     /gene="hscA"
                     /locus_tag="JNZ05_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HAY8565300.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMDKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            22212..22547
                     /gene="fdx"
                     /locus_tag="JNZ05_002063"
     CDS             22212..22547
                     /gene="fdx"
                     /locus_tag="JNZ05_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HAY8565301.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            22559..22759
                     /gene="iscX"
                     /locus_tag="JNZ05_002064"
     CDS             22559..22759
                     /gene="iscX"
                     /locus_tag="JNZ05_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HAY8565302.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            22818..24101
                     /gene="pepB"
                     /locus_tag="JNZ05_002065"
     CDS             22818..24101
                     /gene="pepB"
                     /locus_tag="JNZ05_002065"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HAY8565303.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            24243..25019
                     /gene="sseB"
                     /locus_tag="JNZ05_002066"
     CDS             24243..25019
                     /gene="sseB"
                     /locus_tag="JNZ05_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HAY8565304.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25840..26322)
                     /gene="sseA"
                     /locus_tag="JNZ05_002067"
                     /pseudo
     CDS             complement(25840..26322)
                     /gene="sseA"
                     /locus_tag="JNZ05_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
BASE COUNT         6258 a         6583 c         7310 g         6261 t
ORIGIN      
        1 gttcactaaa ccctattaac tgtaatattt gagcggcacg cgctgccgct catcacaaaa
       61 taattagaat aatccgttgt taataaaaac cctacaggag gcactatgcc taaaaaatga
      121 gaacaacccg taatctatta ttaatggcta cgctattagg tagcgcacta tttgccatgg
      181 ctgcggataa agaaatgacc attggtgcaa tataccttga tacccaggga tattacgctg
      241 gagtgcgtca gggcgttcag gatgcggcaa aagattcttc agtacaggta cagttaattg
      301 aaactaacgc ccagggggat atttcgaaag aaagtacctt tgttgatacc ctcgtggcgc
      361 gtaatgtcga tgccattatt ttatcggcag tgtctgaaaa tggcagtagc cgtaccgttc
      421 gtcgcgccag tgaagcgggt attccggtga tttgctacaa cacctgtatt aatcaaaagg
      481 gtgtcgataa atatgtctcg gcgtatctgg tcggcgatcc actggaattt ggtaaaaaac
      541 tgggtaacgc tgccgccgat tattttattg ccaataaaat tgaccagccg aaaattgccg
      601 tcatcaattg cgaagccttt gaagtttgtg tgcagcgacg taaaggattt gaagaagtat
      661 taaaatcccg cgttcccggc gcgcaaattg tcgctaacca ggaagggact gttttagata
      721 aagcgatttc cgttggtgaa aaactgatta tctccacgcc ggatctcaac gccattatgg
      781 gggagtcggg cggtgcgaca ctcggcgcgg taaaagcggt acgtaatcaa aatcaggccg
      841 gaaaaattgc tgttttcggt tcggatatga caaccgaaat tgctcaggag ctggaaaaca
      901 atcaggtgct gaaagcggta gtggatattt ccggtaagaa aatgggcaat gctgttttcg
      961 cgcaaacatt gaaggttatc aataaacaag ccgacggtga aaaagtgatt caggtgccaa
     1021 tcgatctcta taccaaaacg gaagatggta aacagtggct ggcaacgcac gttgatggtc
     1081 tgccctaatc gctgattcgc agaggtccct atgttcacgg caacagaggc agtcccggta
     1141 gcaaaagtgg tggcaggaaa taagcgttac cccggcgtcg ttgcgctgga taacgttaac
     1201 ttcacgctca ataaaggcga agttcgtgcg ctgttaggta aaaacggcgc gggcaaatcg
     1261 actctcattc gaatgcttac cggcagcgaa cgcccggata gcggtgatat ctggattggc
     1321 gagacgcgac tggaaggtga cgaagctacg ttgactcgcc gtgccgctga actgggggtt
     1381 cgtgcggttt atcaggaatt aagtctggtg gaagggctga cggtggcaga aaacctctgc
     1441 cttggtcaat ggcctcgccg caacggcatg attgattacc tgcaaatggc gcaggatgcc
     1501 caacgttgct tacaggcgct gggcgttgac gttagtcctg aacaacttgt ttcaacgcta
     1561 agcccggcgc aaaagcaact ggtggaaatt gcacgggtga tgaagggcga gccgcgcgtg
     1621 gtcattcttg atgaacccac cagctcgctt gccagtgcag aagttgaact ggtgatcagt
     1681 gcggtgaaaa agatgtcggc actgggcgtg gcggtgattt atgtcagcca ccggatggaa
     1741 gaaatccgcc gcattgcctc ctgtgccacc gttatgcgcg atggtcaggt ggcgggcgat
     1801 gtgatgctcg aaaacacctc cacgcatcat attgtgtcgc taatgctagg gcgtgatcac
     1861 gttgatattg cgccggttgc ccctcaggaa attgtggatc aggccgtact ggaagtccgt
     1921 gcgttacgcc ataagcccaa gctggaggat atcagcttta cgctacgtcg tggcgaagtg
     1981 cttggcattg ctggcctgct gggggcaggg cgcagtgaat tgctgaaggc gattgttggg
     2041 ctggagacgt atgaacaggg cgaaattgtt atcaacggcg agaaaatcat gcgccccgat
     2101 tacggcgaca tgctgaaacg cggcattggc tatacgccag aaaaccgcaa agaagcgggg
     2161 atcattccct ggctgggcgt tgacgaaaat acagtgctga ccaatcggca aaaaatcagc
     2221 accaacggtg tgctgcaatg gtccaccatc cgccgcctga ccgaagaggt gatgcagcgg
     2281 atgacggtca aggctgccag tagcgaaaca cccatcggca cgctttctgg tggcaatcag
     2341 caaaaagtgg tgatcggtcg ttgggtctat gccgccagcc agattttgtt gctcgacgag
     2401 ccaacgcgcg gcgtcgatat cgaagccaaa cagcagattt accgtattgt ccgtgagctg
     2461 gctgccgaag gaaaaagcgt ggtgtttatc tccagtgaag tggaggaatt gccgctggtg
     2521 tgtgaccgca tcctgttatt acagcacggc acgttctcgc aggagtttca ctcgccggtc
     2581 aatgtggatg agctgatgtc cgccattctg tctgtgcact gatgaatttt accgaggaac
     2641 ctgaagatgt ctgcttcgtc attaccattg ccgcagggca agagcgtctc gctcaaacaa
     2701 tttgtcagtc gccatattaa tgagatcggt ttgctggtgg tgattgccat tctttatctg
     2761 gtcttctccc tgaacgcgcc tggctttatc tcattgaata accagatgaa cgtgctgcgc
     2821 gatgccgcca ccattgggat tgccgcctgg gcgatgacgc tgattattat ctccggtgaa
     2881 attgatgtca gcgttgggcc gatggtggct tttgtctcgg tgtgcctggc atttttgctg
     2941 caatttgaag ttccgttggc gattgcttgt ctgctggtgt tgctgttagg cgcgctgatg
     3001 ggaacgctcg ccggggtgct gcgcggcgtg tttaacgtac caagtttcgt tgccacactg
     3061 gggctgtgga gcgctctgcg cggaatgggg ctgtttatga ccaacgcaca gccagtgccg
     3121 attgacgaaa acgaggtgct ggactggctg ggcggacaat ttctcggtgt gccagtatcc
     3181 gcgctgatca tgatggtgtt gtttgcgctg tttgtgttca ttagccgaaa aaccgccttc
     3241 gggcgctcgg tttttgccgt tggcggtaat gccacggcgg cgcagttgtg cggtattaac
     3301 gttcgtcgag tgcgcattct tatctttacc ctttcgggat tattagcggc ggtgaccggc
     3361 attttgttgg cggcacgcct cggttccggt aacgcaggtg ccgcaaacgg tctggagttt
     3421 gacgtcatcg ccgcggtcgt ggtcggcggt acggcacttt ccggcggtcg cggttccctg
     3481 ttcggtacat tgcttggtgt gctggtgatt acgctaatcg gtaacggtct ggtgctgctc
     3541 ggtattaact cctttttcca gcaggtggta cgcggcgtca tcatcgtggt ggcggtgctg
     3601 gcgaatatct tgctgaccca gcgaagcagt aaagcgaaac gctaaacctt aacgcccctg
     3661 gcgcgggtgc ggcagggatg aaaattaatc gtattgttaa tgagggattt atgaaaacga
     3721 tgctggcagc ttatttacta ggaaattcaa ccgtcgatct gcgggaagtt gcggtgccga
     3781 cgccggggat taaccaggta ctgatcaaaa tgaaatcctc cgggatttgc ggaagcgatg
     3841 tccactatat ctatcatcaa caccgtgcca cggcggcggc acccgataaa ccgttatacc
     3901 agggatttat caacggtcat gaaccgtgcg ggcagattgt ggcgatgggg caaggctgcc
     3961 gccattttaa agagggcgac cgcgtgctgg tgtatcacat ttctggctgt ggtttttgcc
     4021 cgaactgccg ccgcggcttt cctatctctt gtactggcaa aggaaaagcg gcttacggct
     4081 ggcagcgtga cggcggtcat gccgaatatc tgctggcgga agaaaaagat ctgatcctcc
     4141 tgccggatgc gctgagctac gaagatggtg cgtttatcag ttgcggcgtt ggcacggcct
     4201 atgaagggat tttgcgcggc gaagtttccg gcagcgataa cgtgctggtg gtcggtctgg
     4261 gggcggtcgg catgatggcg atgatgctgg cgaaaggtcg cggtgcaaaa cggatcatcg
     4321 gcgttgatat gctgccggaa cgtctggcga tggcaaaaca gttagggggg atggatcacg
     4381 gctatttagc aaccaccgaa ggtctgccgc agataattgc tgaacttacc cacggtggcg
     4441 cggatgttgc gctcgattgt tccggtaatg ccgcaggtcg cttgctggca ctgcaatcca
     4501 ctgctgactg ggggcgggtg gtttacattg gtgaaaccgg aaaagtggaa ttcgaggtca
     4561 gcgctgatct gatgcaccat caacggcgga ttatcggctc ctgggtgacc agtctgttcc
     4621 atatggaaaa atgcgcccac gatttgactg actggaagct gtggccgcgt aacgccatta
     4681 cccatcgctt ctcgctagaa caggcaggtg atgcctatgt gctgatggcg agcggcaaat
     4741 gcgggaaagt tgtgattaac ttcccggatt aaggagttag cggatgacca tctatacctt
     4801 atcccacggg tcgctaaagc tggacgtttc tgaccagggc ggcgtaatcg aaggcttctg
     4861 gcgcgatacg acgccgctct tacgtcctgg taaaaaaagc ggtgtggcaa ccgatgcctc
     4921 atgttttccg ctggtgccat ttgcaaaccg ggtgagcggc aaccgttttg tctggcaggg
     4981 gcgtgagtat caactgcaac cgaatgttga gtgggatgcg cactatctac acggcgatgg
     5041 ctggctgggc gagtggcaat gtgtctcgca cagcgatgat agtttgtgtc tggtgtatga
     5101 acatcgcagc ggtgtctatc actatcgggt aagtcaggcg tttcatttaa cggcggatac
     5161 gctgacggtg acgctctctg tcaccaatca aggggcagag acgctgccat ttggtaccgg
     5221 ctggcatcct tattttccgt tgtcaccaca aacgcggatt caggcgcagg cgagcggtta
     5281 ctggctggaa cgggagcagt ggctggcggg tgagttttgc gagcagctac cgcaggaact
     5341 ggattttaac cagctcgcgc cgttgccgcg tcagtgggtg aacaatggtt ttgccggatg
     5401 gaatggtcag gcccgcatcg agcagccgca ggaaggttat gccatcatca tggaaacgac
     5461 gccacctgca ccgtgttatt tcatctttgt ttccgaccct gcgtttgata aaggatatgc
     5521 gtttgatttc ttctgtctgg aaccgatgag ccatgcgccg gatgatcatc atcgcccgga
     5581 agggggtgat ctcattgcgc tagcactagg ggaatcaaca acctcagaga tgtcgttgcg
     5641 ggtagagtgg ctgtaattta taaaatgccg gataaggcgt tcacgccgca tccggcatct
     5701 ttcctaacgc cgatcgagag aaattgcccc cggtccggta attgccaata gcaagaacgc
     5761 gccagcgata ctgacattct tccagaaatt aatcatattt ggcccaaccg catcgccggt
     5821 catatcccag taatggtgac caatcaccgc cgtacccagc gtgtagaaaa taaacagcac
     5881 cgccagcgga cgggtgaaaa agccaagcac gattaatatc gcggcgggca cttccataac
     5941 taccgcaata atcgctgcca gcatcggcat tggcgcgccc aacgaggcca tatattggac
     6001 cgtaccgtca aagcccatca ttttgggaaa accaaaaata atgaaaatta agaccacggc
     6061 gatacgggca attaataaca aaacggggcg ggcagctcca aaatcaaaat aacgtagtgt
     6121 gttcataacc aatccctttg caagataatg tgatttaaac gtaatacaca ctcttgcacc
     6181 tcgccacgcc ccaggcattg agatattcgc taattatcct ggttacagtg atttaagcgc
     6241 gatgttctca tctatcagca gtttcgcatc aaacgttttg ccgctctgga tccatttgcg
     6301 aattgggcga atctcacgcc cctgattcag cgtgaccgca ccgataagca cgccgttttg
     6361 cagattaaac caaatcgcct tctgagtttc cgggttgcca cgacaaagcc agtcatcgcc
     6421 acgcatatcg ccaataaact gtaagttatc actgtactga tcgctccaga accacggcgg
     6481 cggcagtagc ggtagcggta gccctaacat tgcggcagcg gcaatttgcg cctggttatt
     6541 ggcgttttcc cagctttcgc agcggtgtag tgcaccatta tcaagacgag tgattgccac
     6601 atcgccaccg gcaaagatcg cgggatcgca ggtgcggcaa gcctcatcaa tgacaatgcc
     6661 attggcagta tcaaggttgg cctcgcgagc cagttgctcg ttggcgctga tactaatacc
     6721 gtaaatcacc acatcagcct gaagcgtttc cccactttgc agcgtcagtt ctactttttc
     6781 accatcgacc acatgttcaa tggcattatt gagcagaatg cgcacaccag cctgctgatg
     6841 gcgctgtaaa agatagcgtt gcacgggcgg tggtgcatta cggcccatga cggttgccgc
     6901 cagttcaatc actgtcacct tacatctgcg ctgcgctgcg tggcgctggc agccagttcc
     6961 agaccaatag ttccggcacc gacaatcacg actgaccgtt cgggctgcag aacttctcgc
     7021 agtctggcgg catcaccggc atggtgtagg gtaaagcagc gttctcccag tgcatcaagc
     7081 aacggcagcg gtcgagctgc cgcgccggtt gctataaaaa gctgatccca gtgccagctt
     7141 tcgccgttgg ttaacactaa ctctcgtgtg tcgcgaccca gcgttttgat ggttacaccg
     7201 gaatgcagat gaacattgtt ttcctgccac cagttagcgg gtaacacctg ctgtaactgc
     7261 ggggaatctt ccagcaacat ggatttcgag agtggaggtc gttcataagg aagatgtcgc
     7321 tcatcggaaa acagatgcag ctcaccggtg aacccttgct ggcgtagcga ggccgcagcc
     7381 attgccgccg cttgcccgcc accgacaata atgatcgttt tttctttcat cgtgcttcct
     7441 taaagatcca gcccagccgc tacgtggcga atgccgcgaa tcgctaaacc cgcatcagcg
     7501 ttgatcatca caccgcttaa tgcgcgatta ttgcgccgcg atgccaacat cacatacggc
     7561 cccgtaaaat ccgccggttg cgggaaaaat tgcagcggta aaatggcggc aattttctcc
     7621 ggcgtcagag actgcattat cgaggtttca ctttgcccga gcgcctgtgg gccgcgcagg
     7681 tcgctggcca taccacacgg gccgacgcca ttcacccgca ctttcggtgc cagttcataa
     7741 gccagttggc gaataagtcc ggttgcggca tgtttactgg tggtgtacag cgggccaccg
     7801 ccgccaggat accaggcggc atttgacagt gtgaaaatca tgctgccttc actggcgatt
     7861 aacgccggag cgcaggcttt tgcgcccagc aggtaaccga gtacgttgac gttaaacagc
     7921 tcgtggaagc cggtttcgag cgtctctgcg ggagtattaa ccagtgaggc attgtgatcc
     7981 cagatgcctg cattgccgat aaaacaatcc agcttgccgg aacgagtcag gatctgatcg
     8041 accgcgcgtt gataatcggc ataacaggtc acgttacctt ccaccgccag aatatgttcg
     8101 ccaaatcgct gacgcagact ggcgactttt gccgccgaca gttccagcgc ggcaacctgc
     8161 gcgccttctt cgataaatcg ctcgaccagc gccagcccta atcccgatcc gccgccggta
     8221 ataaaaatgg actcgttatg cagatcgctc atggctgcgc ctccggtaag tcgatgaaaa
     8281 tgtcaccacc ttcaacgtgt actggataag tggtgagcgg atcggtggcg ggcaggcata
     8341 acgctttccc cgttttcagg caaaaactgg cggcgtgtag cgggcactcc accgtggcgt
     8401 catcttccag atacccttct gacatcgacg cattaccatg gctgcaacga tcgttaatgg
     8461 cataaaactc gccgcccacg ttgaacaagg cgatgacggg cgaggtatct atccggagag
     8521 cctcaccctc cggcacatcc gcaacgggac acgcataaat tcgattcatc agaacagtac
     8581 actcaggtta tgggaagtga tcaccgcttg atccaggacg atatcccgtt ccagcaagcg
     8641 ccagttgtca tcttccagac ggcgaacttt gtcgaaacgc gttccaacat agaatgtttc
     8701 atcgcgctct ttttgtgccc gataaagtag ataattcacc ctcacggcaa atacgtttgg
     8761 gatgtcggtt tcgcttacct ggcagttgct gattaagtga cgagtgcgtg acggcggctc
     8821 ttctgcctag gccatgcccg tttccagacg ggcgattcgc cgctccagct ggtctttggt
     8881 gtcattgaaa atccaggtcg tcggcggctg gacgcctttg cgacggtcgc gtgtttgcgc
     8941 gttaaccgtg gtgcgcatgg tgtaacgaat ctcttcgtcg agctgtgcca accagtcacg
     9001 aaatttccag tcatccagca gactggcttc gtgaaagaga aactggctaa tgcggtgatg
     9061 taactccagt gaaacttgcg cactcatttc atcacctcct gctggtaagc ggcggtttta
     9121 tcgagcactt cctgccagct ttcgctactc agaagatcgg cccagcgttg gtacattcca
     9181 cgagcggcag tttctgaaaa gatatagtta gtaatgccag gaatgccgtc gtcgcggcgc
     9241 ttttcctgac caagccccat ttccagacac agtttgctgt tgcgggcgcg gtggccttta
     9301 agcaatttct ggatttcaca ccagttctcc gagtcatcct gctcgagaaa accagcagga
     9361 ccaaaagcac gagtggcgct gttttcaaaa gcggctttaa cttcatcgga ggcggctttg
     9421 tcagtaatac agaacgccca cacttcaact tgatcagggc cgcgcggatg ccagacgcgg
     9481 agtgtggcag tgccgtttag ccatgaaagc gtggggaata tattgttatg acccgccagg
     9541 cgcagggcgc gaacttcacc taaacgttgt tctgcttcgg cataggtttc gcggtaatag
     9601 cttgaaactg cgccatcgac ccagacatta gcatccggtt tttcagtaaa gaagaaaccg
     9661 ctaccgtgac cgtcctgacc aaattgcagc gcatctttgg cggtttccca caccgggcgg
     9721 gcggtttgtc catcaccgag gcgcttatcg ctgccatcat ctttcgcccc taatacctga
     9781 acggcagaag catggctgaa cagagcatga tactggtcac tggcgaactg ctctgccggg
     9841 aatttccagt tacagttgat cacccacttt tgtacgccgc cgacaatttc ggtgccgcct
     9901 tcgcgacgat ccagcatgcc atccagatac caggcaatgt cacccaggta atcatgcagg
     9961 cccggtgcgc tggtatccca gttgccaaaa atcagtcctt tataactctc cacacaagga
    10021 acttcgttta gcccccagtg ggatttacac aacccttgtg ggtaggcgcg aggttccagc
    10081 ggtacatcga tcaactcgcc gttaatgcca taagaccagc cgtgatacgg gcaggtaaag
    10141 gcgcgagtgt tgccacaatc tgcataactc acacgcatgg cccggtggcg gcattggtta
    10201 agaaacgcct tgatgctgcc gtctttctgg cgcaccacga caactgcatc ttctcccatg
    10261 taggtgttaa agaaatcacc gggttttggg atctggcttt cgtgggcgag aaatagccag
    10321 caacgaccga aaatacgctc aagctccagt tggtaaatgt ccgggtcggt ataaatacgc
    10381 ggagtgaccc gaccattttg ggtgtcaatc agttggtaaa tgttcaaatc tgagggtgtg
    10441 gtcatatttt ttaatcctga aagataagat gtattgacct tctatttttg tgccataagg
    10501 atgttgctgg ttggttgcta atatgtgaaa tagttaattt ctaccagctg agacgttttt
    10561 tcgataacaa ggggaaggtg atggaactac ggcatttacg ctatttcgtc gcagtggcgc
    10621 aggcactgaa ctttacccgt gcggcggaaa aactgcatac ctcacagcct tcgttaagta
    10681 gccagatccg cgatcttgaa aactgtgtcg gtgttccgtt actggtgagg gataagcgca
    10741 aagtcgcgtt gacggcggcg ggagaatgtt ttctccagga cgcgctggca atcctcgaac
    10801 aagcggaaaa tgccaaatta cgggcgcgga aaattgttca ggaagacaga caattaacca
    10861 ttggctttgt gccatcggcg gaagtgaatt tattgccaaa agtattaccg atgtttcgtc
    10921 tcagacagcc agacaccttg attgagctgg tgagtttaat caccacgcaa caggaggaaa
    10981 aaattcgccg tggtgaactc gacgtcggct taatgcgcca tcctgtttat agcccggaga
    11041 ttgattatct ggagcttttt gacgaaccat tagtggtggt gttaccggtt gatcaccctt
    11101 tagcacatga aaaagagatc accgccgcgc aactggatgg cgtgaatttc gtcagtaccg
    11161 atccggcgta ttccggttcg cttgcgccga tcgttaaagc gtggtttgcg caagaaaaca
    11221 gccagccaaa tatcgtccag gtggcaacga atattctggt gaccatgaat ctggtgggca
    11281 tggggctggg cgtcactttg atacccggtt atatgaataa ttttaatacc gggcaggttg
    11341 tttttcgtcc cattgccgga aatgtgcctt ccattgcttt actgatggcg tggaagaaag
    11401 gggagatgaa gccggcatta cgcgatttca tcgccattgt gcaggaacgt ttggcaagcg
    11461 taacggcata attacgcatt atttttgcaa catgcgccgc ggatcgcagt tttttagtgc
    11521 ttgtgaactg gcgttacaga catcgggaag caggctcgaa tattcttatt tttcaacttg
    11581 caccaacatt cgattaaccc cagactaacg cctcctgacg ggagggactc atggttttgc
    11641 aatccacgcg ctggttggcg ctcggctatt tcacatactt ttttagttac ggcatttttt
    11701 tacctttctg gagcgtctgg cttaaaggga ttggtttaac gccagaaacc atcggcctgt
    11761 tattgggggc aggtctggtt gcccgttttc tcgggagttt gctcatcgcg ccccgcgtca
    11821 gcgatccttc ccgcctgatt tccgccttgc gcgtgctggc actgctgcca cttctctttg
    11881 ctgtcgcctt ctgggcgggg gcgcacgtag cgtggctgat gctggtgatg attggcttta
    11941 acctcttttt ctcaccgctg gtaccgttga ccgatgcact ggcgaatacg tggcaaaagc
    12001 agttcccgct tgattacggc aaagtgcgac tgtggggctc ggtggcgttt gtcattggct
    12061 cggcgctgac gggcaaactg gtcactatgt ttgattatcg ggtgatcctc gtgctgttga
    12121 cgttgggcgt ggcatccatg ctgctcggct ttctcatccg tccgacgatt cagccacaag
    12181 gggcaagccg ccagcaggag agcaccggtt ggtctgcgtg gttggcgctg gttcgccaga
    12241 actggcgctt tctggcctgc gtttgtttat tgcagggggc acatgcggcc tattacggtt
    12301 ttagcgccat ttactggcag gcagctggct actcggcctc ggcggtgggg tatttgtggt
    12361 cgctgggcgt ggtggcggaa gtcattatct ttgcgctgag taataaactt ttccgccgtt
    12421 gtagtgctcg cgatatgctg ttgatctcgg cgatttgcgg cgtagtgcgc tggggcatta
    12481 tgggagcaac tacggcgttg ccgtggttga tagtggtgca aattctgcat tgcggcacct
    12541 tcacggtctg ccacctggcc gccatgcgtt atattgctgc tcgccaggtt agcgaagtca
    12601 tccgtttaca ggcggtttac tctgccgtcg cgatgggcgg cagtatcgct atcatgaccg
    12661 ttttcgccgg tttcctgtat caatatctgg gccacggtgt gttctgggta atggcgctgg
    12721 tggcgcttcc ggcaatgttt ttgcgcccga aagttgttcc ctcatgctga ttcgagcatt
    12781 ttgcggatct gctcctgttg atgtgttgtc agcgtcaggt ccgtatagat cagcggtggt
    12841 gaaaagagcg gtaacggcat ggtataaggc gcaataatca gtgccgcgcc acgcggagcg
    12901 cctgtctgca aaaatgcctt taccgacata tgcttaatgt tgagcggcag taacgttagt
    12961 tcgcgtaact gctgctcaat ctgcgcttct cgctcgctat cattcccggt cagtagaata
    13021 atctgttttt catgcagatc gttttcctgc attagccagg caccgaaaat caccgcgacc
    13081 agaccacttt cctcatcaga aagatggacg ccgtattcac tttcaaatcc ggccagcgcc
    13141 gcgcgggtgg tgcgtaccag gcgtggatac agtctggcga actcttccgg cagggtatta
    13201 tcaataccga tggcaaacaa actgcgattt aacgcctggg cgaggtggat ataaagctga
    13261 tcacataacc cctgttcatc ataaaaacga acatttccca gctcacgaaa atggtttacc
    13321 agacgtttga tagactgacg caattgtctg tcccgctgat gcgcatcccg cagtggatcg
    13381 ggaacccgca acatggaaaa aagtaatgcc ataaacagtg gctcatcagg cggtacatgc
    13441 tggagcgccc gacgctgcca gtggcgtcca atttcctgcg ctacctgaaa ttcaaggcag
    13501 gattccgccc agcgacgttg gagcggatta aactgaggcg taattccagc gtggtgctgc
    13561 agcaaacaat attgcagata cagacacagg aaatgaatat cgcgcgattc aaagtgtttt
    13621 tgcagccgtc gggagcagag attcactaac gcttgtagat tggtatcgtc gtaaaagtta
    13681 cgcgcaattc cgcgccgctt cagttcactc ttcagggcgg gggtgaattg gctggtaata
    13741 aatgacggac acagacggaa accacgtcgt agccagtgaa ataaacacaa acgttggtta
    13801 agcactgtac cttcaacccg gtagctaccg tcataaccag tcgtcagtgt gagttgatga
    13861 tagcgcagga tctcccgtcc tgtttcgctg atatcaagac tggcaatatc atcatccaca
    13921 ccattaagct cgctgaaggt tgccatggtg gcggtcaacc ccggctgaaa gagcgtcagc
    13981 aagatctggc agcggcgctg gggagccgaa aggacagatg gtggagcaag cgtaggcatc
    14041 atctgtagat ctcccataag cataattgat aagaatagct aaagtttgct cagggaaatg
    14101 gcgaagggaa tgctaatcat cagaaatgtt ggcaagcgtc acagaatttt tctcatttta
    14161 aactgttctg accagtggat tagcatgtct taaggtggtt ttgtgaatga ttatcgtgct
    14221 agcgatcatg ggttagttct gcggattcaa cgcgctaagg ataaagtcca ccatctgttc
    14281 gcgataaaca ttgccgtgac gatcggaaaa agcatcaatg tgttcgccat ccgggattag
    14341 gatcaaccgt tttggctctt ttgccaggct atacaacttt tcgctgtgct gccatgggat
    14401 aacgtgatcg gctttaccgt gaatgagtaa aagcgggatc gggctgacgc tggcgatgta
    14461 attttcgccg ctgtaactct catcaagtaa gtagccactg ccggggatca tctggttggc
    14521 gatggttgca taagaggcaa atgtggagtc gaggatcacc gcacgtatgc cttcacgatc
    14581 accctgacca ataacagcaa gaatattcgc cccgccaata ctctgcccga acagcaccag
    14641 ccgttgtggg tttacatcac tgcgatggcg caccacattg atggcgcttt gcgtatcgtc
    14701 cagcaatccg gcctgggacg gagtgccttt tgatttacca aacccgcgat aatcaaacat
    14761 aaaaacgttg aaattacgct caggtaacca actgaccagc ggccagtggg cggacatatt
    14821 tccggcattg ccgtgagcat gaatgatggt tgcgatggcg ttgtcagcag ggctcgtcga
    14881 agaaggaata aaccagcctt gcagacgagt accgtcctta gccgtaaatt cgacggattc
    14941 cgccgaccag gggtcgggac cgtaaatttt atcgtcagga taatagaaga agttgatggc
    15001 aacgcgcagc actaaataaa cgcagaaggc aacaacaaag agaataaaca gaattttggg
    15061 aacgcgtttg ttcactggca gtgccataac ctttccttgc gacttcttta tcatccatta
    15121 atcggggaaa gatggtggga gagtgacgaa agaaagtaaa gcggtgagga agtatttcgg
    15181 aatagcgcct gaaaggcgag ggcgggtgag tgatatcacc cgcctgagtc attaacgctt
    15241 cagagcgtcg cttaactcgt cacgcatgtt cgccagtatg gctttcacaa cacgcgggtt
    15301 accagcaacg atgttaccgg tcagcatata gttatgacca ccggtgaagt cgctgacgat
    15361 gccgcccgct tcacgaacca gcagctcgcc tgccgcgaaa tcccacggac gcagaccgat
    15421 ttcaaagaaa ccgtcaacac gacccgcagc gacgtaagcc agatccagcg ccgcagaacc
    15481 ggtacgacgg aagtctgcac attcgttgaa cagcttgccg acgatgttga tgtaggtagt
    15541 ggcgtactgt tttgctttga acgggaagcc ggtcgccaga atggtaccgt cgagatcgcg
    15601 agcggtgcta ccgcgcagac ggtagccgtt cagctgtgcg ccctgaccgc gagtggcggt
    15661 gaacagttcg ttacgcatag gatcgtatac cacagcaact tcggtgcggc ctttgatacg
    15721 aacggcgata gataccgcga agtgcggcag acgtttgata aagttggtag taccatccag
    15781 tggatcgata acccattgaa catcctgatc agtaccttca agttcaccgc tttcttcggt
    15841 gatgatggtg tgctgtgggt aagatttacg aatcgtgtcg ataatcaccg cttcggcagc
    15901 tttatctacg ttggtcacga aatcgttact gcctttctgg ctcgcttcta cagcgtccgg
    15961 ggtttcatag tttttggcaa ttaaattacc cgccttgcgc gctgcgcgca cggcgatgtt
    16021 cagcatcgga tgcatcggtc tctctcactg gatgttaaag aacgggaaaa cggcgcagag
    16081 tatagcagcg agatcggcat ctgtcttcgt tttatgataa gatgcgtgaa taatctttag
    16141 atgaagaaag acaatgctgc aaaatattcg aattgtgctg gtggagacgt cacacaccgg
    16201 caatatgggt tctgttgccc gtgccatgaa aacaatggga ttaaccaatc tgtggctggt
    16261 taatccactg gtgaaacccg actcccaggc gattgccctg gctgcagggg ccagcgatgt
    16321 gattggtaat gctcacatcg tcgatacgct cgacgaagcg ttagccggtt gtagcctggt
    16381 ggtgggcacc agcgcacgct cccgcacgct gccgtggccg atgctcgacc cgcgcgaatg
    16441 cggtctgaaa agcgtcgctg aagcggcaaa taccccggtg gcgctggtgt ttggtcgtga
    16501 gcgcgtcggc ttgaccaatg aagagttgca gaaatgccat tatcatgtcg cgattgcggc
    16561 aaacccggaa tacagttcgc tgaacctggc aatggcggtt caggttattg cctatgaagt
    16621 gcgtatggcc tggctggcaa ctcaggaaaa tggcgagcag gtcgagcatg aagagacgcc
    16681 gtatccgctg gtcgatgatc tggagcgttt ttatggtcat ctggaacaaa cgctgctggc
    16741 aaccggtttt atccgtgaaa accatccggg gcaggtgatg aataaattgc gccgtctgtt
    16801 tacccgtgcg cgcccggaaa gccaggaatt gaatatcctg cgcgggattc tggcttctat
    16861 tgagcagcag aataaaggta acaaggccga ataacagtgg ttgcctgatg cgacgcttaa
    16921 cgcgtcttat caggcctaca atgtacagaa tcgtaggtcg gataaggtgt tcacaccgca
    16981 tccgacaaca ggtacaaacg ccacgataaa aaaatggcac tgaaggttaa atacccgact
    17041 aaatcagtca agtaaatagt tgaccaattt actcgggaat gtcagacttg accctgctat
    17101 gcaatacccc cacttttaca ataaaaaacc ccgggcaggg gcgagtttga ggtgaagtaa
    17161 gacatgagac tgacatctaa agggcgctat gccgtgaccg caatgcttga cgttgcgctc
    17221 aactctgaag cgggcccggt accgttggct gatatttccg aacgtcaggg aatttccctt
    17281 tcttatctgg aacaactgtt ttcccgtctg cgtaaaaatg gtctggtttc cagcgtacgt
    17341 ggaccaggcg gtggttatct gttaggcaaa gatgccagca gcatcgccgt tggcgaagtg
    17401 attagcgccg ttgacgaatc tgtagatgcc acccgttgtc agggtaaagg cggctgccag
    17461 ggcggcgata aatgcctgac ccacgcgctg tggcgtgatt tgagcgaccg tctcaccggt
    17521 tttctcaaca acattacttt aggcgaactg gttaataacc aggaagtgct ggatgtgtct
    17581 ggtcgtcagc atactcacga cgcgccacgc acccgcacac aagacgcgat cgacgttaag
    17641 ttacgcgctt aataaaaaga attcagaatc aggccggagt gctaaatact ccgtaaacac
    17701 ggtcgtacat ccagccggta gcctgattcc ttgcattgag tgatgtacgg agtttataga
    17761 gcaatgaaat taccgattta tctcgactac tccgcaacca cgccggtgga cccgcgtgtt
    17821 gccgagaaaa tgatgcagtt catgacgatg gacggaacct ttggtaaccc ggcctctcgt
    17881 tctcaccgtt tcggctggca ggctgaagaa gcggtagata tcgcccgtaa tcagattgcc
    17941 gatctggtcg gcgctgatcc gcgtgaaatc gtctttacct ctggtgcaac cgaatctgac
    18001 aacctggcga tcaaaggtgc agccaacttt tatcagaaaa aaggcaagca catcatcacc
    18061 agcaaaaccg aacacaaagc ggtactggat acttgccgtc agctggagcg cgaaggtttc
    18121 gaagtcacct acctggcacc gcagcgtaac ggtattatcg acctgaaaga actcgaagcg
    18181 gcgatgcgtg acgacaccat ccttgtttcc atcatgcacg taaataacga aatcggcgtg
    18241 gtgcaggata tcgcggctat cggcgaaatg tgccgtgctc gtggcattat ctatcacgtt
    18301 gatgcaaccc agagcgtggg caaactgcca atcgacctga gccagttgaa agttgacctg
    18361 atgtctttct ccggtcacaa aatctatggc ccgaaaggta tcggtgcgct gtatgtgcgt
    18421 cgtaaaccgc gtgtacgcat cgaagcgcaa atgcacggcg gcggtcacga gcgcggtatg
    18481 cgttccggca ctctgcctgt tcaccagatc gtcggaatgg gcgaagccta tcgcatcgca
    18541 aaagaagaga tggcgaccga gatggaacgt ctgcgcggcc tgcgtaaccg tctgtggaac
    18601 ggcatcaaag atatcgaaga agtttacctg aacggtgacc tggaacacgg tgcgccgaac
    18661 attctcaacg tcagcttcaa ctacgttgaa ggtgagtcgc tgattatggc gctgaaagac
    18721 ctcgcagttt cttcaggttc cgcctgtacg tcagcaagcc tcgaaccgtc ctacgtgctg
    18781 cgcgcgctgg ggctgaacga cgagctggca catagctcta tccgtttctc tttaggtcgt
    18841 tttactactg aagaagagat cgactacacc atcgagttag ttcgtaaatc catcggtcgt
    18901 ctgcgtgacc tttctccgct gtgggaaatg tacaagcagg gcgtggatct gaacagcatc
    18961 gaatgggctc atcattaatc ggtatcggaa tcaggagaat ttataatggc ttacagcgaa
    19021 aaagttatcg accattacga gaatccgcgt aacgtgggtt cctttgataa caacgacgag
    19081 aacgtcggca gcggcatggt gggtgcaccg gcctgtggcg acgtaatgaa gttgcagatt
    19141 aaagtcaacg atgaaggtat cattgaagac gcgcgtttta aaacttacgg ctgcggttcc
    19201 gctatcgctt ccagctccct ggtcaccgaa tgggtgaaag ggaagtctct cgacgaagcg
    19261 caggcgatca aaaacaccga tattgctgaa gaacttgaac tgccgccggt gaaaattcac
    19321 tgttctattc tggcagaaga cgcgatcaaa gccgccattg cggactataa aagcaaacgt
    19381 gaagcaaaat aagagttgag gtttggttat gtcgattaca ctgagcgaca gtgcagcagc
    19441 gcgagtaaat acctttctgg ctaaccgcgg caaagggttt ggcctgcgtc tgggcgtgag
    19501 aacctccgga tgttcaggta tggcttatgt actggaattt gttgacgaac cgacgccgga
    19561 agacatcgtg tttgaagaca aaggcgtgaa agtcgtggtc gatggcaaaa gcctgcaatt
    19621 tctggacggt acgcagctgg acttcgtaaa agaaggcctg aatgaagggt ttaaattcac
    19681 caacccgaac gttaaagatg agtgtggttg cggcgaaagc ttccacgttt gatgcgcata
    19741 ccgataaccc caccgtggtc gcctgcgcgt ggggtttgtt ttacctgatt cgccgttatc
    19801 gcggcggatc gcagccctga gaatgttatg gattacttca ccctctttgg cttgcctgcc
    19861 cgttatcaac tcgataccca ggcactgagc ctgcgttttc aggatctaca acgtcagtat
    19921 catcctgata aattcgccag cggaagcctg gcggaacaac tcgccgccgt acagcaatct
    19981 gcaaccatta accaggcctg gcaaacgctg cgtcatccgt taatgcgcgc ggaatatttg
    20041 ctttctttac acggttttga tctcgccagc gagcagcata ctgtgcgcga caccgcgttc
    20101 ctgatggaac agttggagct gcgcgaagag ctggacgaga tcgaacaggc gaaagatgaa
    20161 gcgcggctgg aaagctttat caaacgtgtg aaaaagatgt ttgatacccg ccatcagttg
    20221 atggttgaac agttagacaa cgaggcgtgg gacgcggcgg cggatacggt gcgtaagctg
    20281 cgttttctcg ataaactgcg gagcagtgcc gaacaactcg aagaaaaact gctcgatttt
    20341 taatttctgg aagctaaaca tggccttatt acaaattagt gaacctggtt tgagtgctgc
    20401 gccgcatcag cgtcgtctgg cggctggtat tgacctgggc acaaccaact cgctggtggc
    20461 gacagtgcgc agcggtcagg ccgaaacgtt agccgaccat gaaggccgtc acctgctgcc
    20521 atctgttgtt cactatcaac agcaagggca ttcggtgggt tatgacgcgc gtactaacgc
    20581 agcgcttgac accgccaaca ccattagttc tgttaaacgc ctgatggggc gctcgctggc
    20641 tgatatccag caacgctatc cgcatctgcc ttatcaattc caggccagcg aaaacggcct
    20701 gccgatgatt gaaacggcgg cggggctact gaacccggtg cgcgtttctg cggacatcct
    20761 caaagcactg gcggcgcggg caactgaagc cctggcaggc gagctggatg gtgtagttat
    20821 caccgttccg gcgtactttg acgatgccca gcgtcagggc accaaagacg cggcgcgtct
    20881 ggcggggttg catgttctgc gcttacttaa cgaaccgacc gctgcggcta tcgcctacgg
    20941 gctggattcc ggtcaggaag gcgtgatcgc tgtttatgac ctcggtggcg ggacgtttga
    21001 tatttccatt ctgcgcttaa gtcgcggcgt gtttgaagtg ctggcaaccg gcggtgattc
    21061 cgcgctcggc ggcgatgatt tcgaccatct gctggcggat tacattcgcg agcaggcgga
    21121 cattcctgat cgtagcgata accgcgttca gcgtgaactg ctggatgccg ccattgcagc
    21181 caaaatcgcg ctgagcgatg cggactccgt gaccgttaac gttgcgggct ggcagggcga
    21241 aatcagccgt gaacaattca atgaactgat cgcgccactg gtaaaacgaa ccttactggc
    21301 ttgtcgtcgc gcgctgaaag acgcgggtgt agaagctgac gaagtgctgg aagtggtgat
    21361 ggtgggcggt tctactcgcg tgccgctggt gcgtgaacgg gtaggcgaat ttttcggtcg
    21421 tccaccgctg acttccatcg acccggataa agtcgtcgct attggcgcgg cgattcaggc
    21481 ggatattctg gtgggtaaca agccagacag cgaaatgctg ctgcttgatg tgatcccact
    21541 gtcgctgggc ctcgaaacga tgggcggcct ggtggagaaa gtgattccgc gtaataccac
    21601 tattccggtg gcccgggctc aggatttcac cacctttaaa gatggtcaga cggcgatgtc
    21661 tatccatgta atgcagggtg agcgcgaact ggtgcaggac tgccgctcac tggcgcgttt
    21721 tgcgctgcgt ggtattccgg cgctaccggc tggcggtgcg catattcgcg tgacgttcca
    21781 ggtcgatgcc gacggtcttt tgagcgtgac ggcgatggat aaatccaccg gcgttgaggc
    21841 gtctattcag gtcaaaccgt cttacggtct gaccgatagc gaaatcgctt cgatgatcaa
    21901 agactcaatg agctatgccg agcaggacgt aaaagcccgt atgctggcag aacaaaaagt
    21961 agaagcggcg cgtgtgctgg aaagtctgca cggcgcgctg gctgctgatg ccgcgctgtt
    22021 aagcgccgca gagcgtcagg tcattgacaa tgctgccgct cacctgagtg aagtggcgca
    22081 gggcgatgat gttgacgcca tcgaacaagc gattaaaaac gtagacaaac aaacccagga
    22141 tttcgccgct cgccgcatgg accagtcggt tcgtcgtgcg ctgaaaggcc attccgtgga
    22201 cgaggtttaa tatgccaaag attgttattt tgcctcatca ggacctctgc cctgatggcg
    22261 ctgttctgga agctaatagc ggtgaaacca ttctcgacgc agcgctgcgt aacggtatcg
    22321 agattgaaca cgcctgtgaa aaatcctgtg cttgcaccac ctgccactgc atcgttcgtg
    22381 aaggttttga ctcactgccg gaaagctcag agcaggaaga cgacatgctg gacaaagcct
    22441 ggggactgga gccggaaagc cgtttaagct gccaggcgcg cgtcaccgac gaagatttag
    22501 tggtcgaaat cccgcgttac actatcaacc atgcgcgtga gcattaacag aggttagtat
    22561 gggacttaag tggaccgata gccgcgaaat tggcgaagca ctgtacgatg cgtatcccga
    22621 tcttgatccg aaaacggttc gattcaccga tatgcatcag tggatttgcg atctggaaga
    22681 tttcgacgac gacccgcagg catccaacga gaaaatcctc gaagcgattt tgttagtctg
    22741 gctggacgaa gccgagtgaa tgaacgggct gcctgggggt agcccgtttt gctgttaaac
    22801 gaataaggat aactaaaatg acagaagcga tgaagattac cctctctacc caacctgccg
    22861 acgcgcgctg gggagaaaaa gcaacttaca gcattaataa tgacggcatt accctgcatt
    22921 tgaacggggc agacgatctg gggctgatcc agcgtgcggc ccgcaagatt gacggtctgg
    22981 gcatcaagca tgttcagtta agcggtgaag gctgggatgc ggatcgctgc tgggcattct
    23041 ggcaaggtta taaaggcccg aaaggcacgc gtaaagtgga gtggccggat ctggacgatg
    23101 cccagcgcca ggaactggat aaccgcctga tgatcatcga ctgggtgcgt gacaccatca
    23161 acgcaccggc agaagaattg ggaccatcgc aactggcaca gcgtgctgtt gatctgatca
    23221 gcaacgtcgc gggcgatcgt gtgacttatc ggatcaccaa aggcgaagat ctgcgtgagc
    23281 aaggttatat ggggctgcac actgtcgggc gcggttcaga acgttctccg gtattgctgg
    23341 cgctggatta caacccaact ggcgataaag aagcaccggt gtacgcgtgc ctggtaggta
    23401 aaggtatcac ttttgactcc ggcggctaca gcatcaaaca gactgcgttt atggactcga
    23461 tgaagtcgga catgggcggc gcggcaacgg ttaccggggc gctggcattt gccattacgc
    23521 gcggactgaa caagcgcgtg aagctgttcc tctgctgtgc ggataacctg attagcggca
    23581 atgcgttcaa gctgggcgat atcatcacct atcgcaacgg taaaaaagtt gaagtgatga
    23641 acactgatgc cgaagggcgt ctggtgcttg ccgatggtct gattgatgcc agtgcgcaga
    23701 aaccggaaat gatcattgat gcggcgaccc tcaccggggc ggcgaaaact gcgctgggta
    23761 atgattatca cgcgctgttc agttttgacg atgcgcttgc cggtcgttta ctggcgagtg
    23821 cctcacaaga gaacgaacca ttctggcgtc tgccgctggc ggaattccac cgcagccagc
    23881 tgccgtctaa ctttgccgaa ctgaacaata ccggaagcgc ggcgtatccg gcaggcgcga
    23941 gcacggcggc gggcttcctg tcgcactttg ttgagaacta tcagcaaggc tggctgcata
    24001 tcgactgctc ggcgacttac cgtaaagcgc cggttgaaca gtggtctgcg ggcgctacgg
    24061 gacttggtgt gcgcacgata gctaatctgt taacggcgta aactaatatg ccggatgcgg
    24121 cgtaaacgcc ttatccggcc tacaaattct gcaaattcaa aaaattgcga atcccttgta
    24181 ggcctgataa gcgtagcgca tcaggcattt gcgccccgaa cgttgaatga attgtggttc
    24241 ttatgtccga aacaaaaaac gaacttgaag acctgctgga aaaagcagca accgaaccgg
    24301 cgcaccgccc ggcctttttc cgtactctac tggaatccac cgtctgggtg cctggtacgg
    24361 cggcgcaggg cgaggctgtg gttgaagata gtgcgcttga tttacagcac tgggaaaaag
    24421 aagacggcac cagcgtcatt ccttttttca cctcgttaga agcgcttcaa caggcggttg
    24481 aagacgaaca ggcatttgtc gtaatgcccg ttcgcacatt gtttgagatg acccttggcg
    24541 aaacgctctt ccttaatgcc aaactgccga ccggtaaaga atttatgccg cgtgaaatca
    24601 gtttgttgat tggtgaagag ggaaatccgc tgagcagcca ggaaatcctg gaaggcggtg
    24661 aatcgctgat attatcggaa gtcgcagagc cgccagcaca aatgattgat tcactcacca
    24721 ccttatttaa aaccattaag ccggtgaagc gtgcttttat ttgttcaatt aaagagaacg
    24781 aagaggcaca gcctaattta cttattggca ttgaagccga tggtgatatc gaagaaatta
    24841 ttcaggcgac gggaagtgta gcgaccgata cattacctgg cgatgaacca atcgatattt
    24901 gtcaggtgaa aaaaggggaa aaaggaatta gccactttat taccgaacat attgcgccat
    24961 tctatgaacg tcgctggggt ggttttttgc gtgactttaa acaaaaccgg ataatctaaa
    25021 atatgccgcc ctaaagggcg gcattcgggc ttacttgctg ctctgaaggt tgttaaccac
    25081 cagtaaattg tcgccggaga gactccggtt attatttact tgggaaaaca gcgcaccaga
    25141 aacaataagt acgatacaga agcaccgtat cttcattgtg ctgtcgtgat gcgaagaccg
    25201 catcacgaca gcaccactgg cgatattgcc gcgatagcaa gcgctgagac gcgcttgctc
    25261 taacggtcgc ggaacatcct ttccttaagc cagcgcccgc ttgcgagacg gcggaaaggg
    25321 ccgcagtgga taatacacta acagaacaat aataactctc ttttgcttga caaaaagaga
    25381 gttactggcg agtattgtct ccgctgagac gcgcttgcat tactacggta atacaacctg
    25441 ctggaaaggc cgcgaaccgg accagcaaca aataaccgcc tgattttggc ggttttttgt
    25501 tttttaaagg aaagtaaatt tacatgttta agtaatatct tttaccggtt agaattttta
    25561 attttgagca ggatttgttt atatttgtct gaattcggac taataagcca ggttatttta
    25621 ttaccgaaca cggcggaaag ggccggtgtc gataatacgc taaataaaca ataattactc
    25681 tcttttgctt gacaaaaaga gagttactgg tgagtattgt tttactgtga agcacctgcg
    25741 ttgctcatgt aacacaagtc aacctgctgg aaaggccgcg aaccagacca gcaataaaaa
    25801 aacgccaaat tcggcggttt tttattgcct gtaaaatact tatttcactg gctcaaccgg
    25861 taaatctgcc cgcgcgcccc attcactcca tgcgccgtcg tacagtttca cgtttggcac
    25921 atccagcgtc gcgagtgcta acaaaaccac ggctgccgtt acaccagagc cgcagctgac
    25981 gataatcggt ttgtcatagc tgacgccgcg accaaaaaat atcgcatcca gttcatcggt
    26041 cgtttttagt tcgccttcgc gcaccagttc cgtccacgga acgttcaatg caccgggaat
    26101 atgtccgcga cgtaaacctg ggcgaggttc atcaacttct gcgttaaaac gtgcagccgg
    26161 gcgggcatca ataatttgcg ccgtattttc atggcttgcc aataacacat cggttacttt
    26221 caccactgct tcaggattaa acgcggcgtt aaactctcct tccggcagct ctactgcacc
    26281 ttcttctaat agcaggtcat cgcgctgcca gcctgcaagt ccccgttact ttattcgtac
    26341 cccttataat ggggtgttag ccagccagac ccggcatgat tactgccccc agtcgtccat
    26401 gatccggggg gg
//
LOCUS       DADMEQ010000049        25899 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.049, whole genome shotgun sequence.
ACCESSION   DADMEQ010000049 DADMEQ010000000
VERSION     DADMEQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.049"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..343)
                     /gene="yeiL"
                     /locus_tag="JNZ05_002068"
     CDS             complement(<1..343)
                     /gene="yeiL"
                     /locus_tag="JNZ05_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HAY8565305.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHY"
     gene            474..1415
                     /gene="rihB"
                     /locus_tag="JNZ05_002069"
     CDS             474..1415
                     /gene="rihB"
                     /locus_tag="JNZ05_002069"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HAY8565306.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            1515..2765
                     /locus_tag="JNZ05_002070"
     CDS             1515..2765
                     /locus_tag="JNZ05_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY8565307.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLVQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(2821..3909)
                     /locus_tag="JNZ05_002071"
     CDS             complement(2821..3909)
                     /locus_tag="JNZ05_002071"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY8565308.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLVDCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3912..4769)
                     /gene="nfo"
                     /locus_tag="JNZ05_002072"
     CDS             complement(3912..4769)
                     /gene="nfo"
                     /locus_tag="JNZ05_002072"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAY8565309.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(4843..5892)
                     /locus_tag="JNZ05_002073"
     CDS             complement(4843..5892)
                     /locus_tag="JNZ05_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HAY8565310.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            5991..6872
                     /locus_tag="JNZ05_002074"
     CDS             5991..6872
                     /locus_tag="JNZ05_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8565311.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            7078..8547
                     /gene="lysP"
                     /locus_tag="JNZ05_002075"
     CDS             7078..8547
                     /gene="lysP"
                     /locus_tag="JNZ05_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HAY8565312.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVLAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            8839..10830
                     /gene="cirA"
                     /locus_tag="JNZ05_002076"
     CDS             8839..10830
                     /gene="cirA"
                     /locus_tag="JNZ05_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HAY8565313.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(10862..11698)
                     /gene="yeiG"
                     /locus_tag="JNZ05_002077"
     CDS             complement(10862..11698)
                     /gene="yeiG"
                     /locus_tag="JNZ05_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HAY8565314.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATYYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSAPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            11956..12624
                     /gene="folE"
                     /locus_tag="JNZ05_002078"
     CDS             11956..12624
                     /gene="folE"
                     /locus_tag="JNZ05_002078"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAY8565315.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            12641..13798
                     /locus_tag="JNZ05_002079"
     CDS             12641..13798
                     /locus_tag="JNZ05_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HAY8565316.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWWLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGLAISKTSR"
     gene            13940..14908
                     /gene="galS"
                     /locus_tag="JNZ05_002080"
     CDS             13940..14908
                     /gene="galS"
                     /locus_tag="JNZ05_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HAY8565317.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNTNAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRAATVLCRR"
     gene            15259..16257
                     /gene="mglB"
                     /locus_tag="JNZ05_002081"
     CDS             15259..16257
                     /gene="mglB"
                     /locus_tag="JNZ05_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HAY8565318.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            16318..17838
                     /gene="mglA"
                     /locus_tag="JNZ05_002082"
     CDS             16318..17838
                     /gene="mglA"
                     /locus_tag="JNZ05_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HAY8565319.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIGMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            17854..18864
                     /gene="mglC"
                     /locus_tag="JNZ05_002083"
     CDS             17854..18864
                     /gene="mglC"
                     /locus_tag="JNZ05_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HAY8565320.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(18900..20135)
                     /gene="preA"
                     /locus_tag="JNZ05_002084"
     CDS             complement(18900..20135)
                     /gene="preA"
                     /locus_tag="JNZ05_002084"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HAY8565321.1"
                     /translation="MLTKDLSITFCGVNFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLTALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVKKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(20129..21368)
                     /locus_tag="JNZ05_002085"
                     /pseudo
     CDS             complement(20129..21368)
                     /locus_tag="JNZ05_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(21562..21801)
                     /locus_tag="JNZ05_002086"
     CDS             complement(21562..21801)
                     /locus_tag="JNZ05_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HAY8565322.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(21804..22523)
                     /gene="sanA"
                     /locus_tag="JNZ05_002087"
     CDS             complement(21804..22523)
                     /gene="sanA"
                     /locus_tag="JNZ05_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HAY8565323.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(22673..23557)
                     /gene="cdd"
                     /locus_tag="JNZ05_002088"
     CDS             complement(22673..23557)
                     /gene="cdd"
                     /locus_tag="JNZ05_002088"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAY8565324.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(23687..24382)
                     /locus_tag="JNZ05_002089"
     CDS             complement(23687..24382)
                     /locus_tag="JNZ05_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HAY8565325.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMSVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKVARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(24379..24777)
                     /locus_tag="JNZ05_002090"
     CDS             complement(24379..24777)
                     /locus_tag="JNZ05_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAY8565326.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            25016..>25899
                     /gene="dusC"
                     /locus_tag="JNZ05_002091"
     CDS             25016..>25899
                     /gene="dusC"
                     /locus_tag="JNZ05_002091"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HAY8565327.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVR"
BASE COUNT         6365 a         6424 c         6738 g         6372 t
ORIGIN      
        1 ggtaatgttt catagggagc gcaaggcacc aacactcttc aatagcctgc accgcacgcg
       61 gttcatggtc tttgtcgatt aactcaatct cgccaataaa acagggagca gcaaagaaat
      121 cgatcagcga cacgcgacca ttagcaagcg tggcgtaaag cctggcgcgg cctcgcgtca
      181 gataaaacag ccatgacggc tgttgccctt cctgcacaat gtagtcacgc gctaaaaaat
      241 gaaacagccg cgtatcggct gaaacatccg ttgaaaagca atccttaaac gcagattcac
      301 tcatcaattg ctgcttaagg tcattattgt ggatttcttt cacgatgttt atccgctatg
      361 agatttctca tatttagcat acgtacgctg ttgttaagat tcaatcatca gcccggtcga
      421 attttctttg ctgtagaaaa aaataacgca actggaaaca gaggaaataa acaatggaaa
      481 agagaaaaat tattctggat tgtgagccgg gtcatgatga tgctattgca atgatgatgg
      541 cagcgaaaca tccggcaata gatttattag gcatcactat tgtagcgggt aatcagacgc
      601 ttgataaaac attaattaat ggcctgaatg tttgccagaa actggagatt aatgttccgg
      661 tttatgcggg gatgccgcaa cccattatgc gtaaacaaat cgttgccgat aatattcacg
      721 gtgaaaccgg actggatggc ccggtattcg agccgctgac ccgccaggca gaaagtaccc
      781 atgcggtgaa atatatcatc gataccctga tggcaagcga tggcgatatc actctggtgc
      841 cggttggtcc gctttcaaat atcgcggtgg caatgcgtat gcaacccgca atcctgccca
      901 aaatccgtga aattgtgcta atgggcggcg cttacggtac aggcaacttc accccatctg
      961 ccgagttcaa tatctttgcc gacccggaag ccgcccgcgt ggtgttcacc tccggcgttc
     1021 cattagtgat gatgggcctc gatcttacca accagaccgt ttgcaccccg gatgtaattg
     1081 ctcggatgga aagggtaggc ggccctgccg gagagctgtt cagcgacatc atgaacttca
     1141 ctctcaaaac gcagttcgaa tactacggcc tggctggcgg cccggtgcat gacgccacct
     1201 gcattggtta tctgatcaac cctgatggca ttaaaaccca ggatatgtac gtcgaagtgg
     1261 acgtcaacag cggcccttgc tacgggcgta cggtctgcga cgaactgggc gttcttggca
     1321 agcccgctaa taccaaagtc ggcatcacta ttgataccga ctggttctgg ggcttagtcg
     1381 aagagtgcgt gcgcggctac atcaaaaccc attaagtgtt gataacaagc cgggacagta
     1441 tcgcaagatg caggcccggc tatttctgat gggcagaaag ccgtcgccca aaatgacatg
     1501 aagagataat cactatggat gtcatgagaa gtgttctggg aatggtggta ctgctggcaa
     1561 ttgcgttttt actgtcagta aacaagaaga agatcagcct gcgtaccgtt ggcgcggcgt
     1621 tagtgttaca ggtcgtgatt ggcgggatta tgctttggtt accgccaggg cgttgggtcg
     1681 cagaaaaagt cgcttttggc gtccacaaag tgatggcgta cagcgacgca ggtagcgcat
     1741 ttatcttcgg ttctctggtc ggaccgaaaa tggatacctt atttgatggc gcaggattta
     1801 tctttggttt cagggtatta ccggcaatta tcttcgtcac tgcactggtg agtattctct
     1861 actacatcgg tgtgatgggg atcttaattc gcattctcgg cggtatcttc cagaaagctt
     1921 taaatatcag caagatcgag tcattcgtcg cggtcaccac cattttcctc gtgcaaaacg
     1981 aaattccggc gatcgtcaaa ccctttatcg atcgtctgaa tcgcaatgaa ttatttacag
     2041 cgatttgtag tggcatggcc tcgattgctg gttcgacaat gattggttac gccgcactgg
     2101 gcgtacctgt ggaatatttg ctggcggcat cgttaatggc gatccctggc gggatcttgt
     2161 ttgcccgcct gttaagcccg gctacggaat cttcgcaggt ttcttttaat aacctctctt
     2221 tcaccgaaac accgccaaaa agcattattg aagccgatgc gacaggggca atgaccgggc
     2281 tgaaaatcgc cgccggtgta gcgacagttg ttatggcatt tgtcgccatc attgcgttaa
     2341 ttaacggtat tatcggcggc gttggcggct ggtttggttt tgaacatgcc tcgctggagt
     2401 ccattttagg ttacctgctg gccccacttg cgtgggtaat gggtgttgac tggagtgatg
     2461 caaatcttgc cgggagtttg attggacaga aactggcaat aaatgaattt gtcgcttatc
     2521 tcaatttctc accctatctg caaacggctg gcactctgga tgctaaaacc gtggcgatta
     2581 tttccttcgc gttgtgcggt ttcgctaact ttggttctat cggggtggtg gtgggggcgt
     2641 tttctgcggt tgcgccacac cgtgcgccgg aaatcgccca gcttggttta cgcgcgctgg
     2701 cggcggcgac actttctaat ctgatgagtg ccaccattgc cgggttcttt attggtttag
     2761 cgtagtgtgc aatttatgag ccggattaga tgcggtcaag cgtcgaatcc ggcaacattg
     2821 ttacaccatt ggcactaatg acagcgcgtt atcggcagac agggtagggt tgtttaagct
     2881 gccgctggcg cgtgaaattg ccgcgcaggc catcgcaaaa cgggcgctgt cacggaaatt
     2941 gtttccctcc agaaagctat ataccagacc cgccataaaa ccatcgtcag caccaaaact
     3001 gtctaccgtc gtatgcgctg gcacagtcag caaaaattgt tcaccatcct tttcgctgca
     3061 ataaactgac tcatcgggca aataaacaaa cagttgctga acaccttgct gatgcaatgc
     3121 attcactgcg gcattacggt cagcatcgct ggtgatcgcc tgtccccata aaatttccag
     3181 ctccggtaaa gtgggtttca gggtgtgaat atgcgccagc cagtgtttga ttttgcccgc
     3241 tttgaattct gaaacggtat cgacaaacac cgggatctca tcagcgaggg taaagaccca
     3301 ttccagcgcc tcggctgtca ggttgcaatc aaccagtacc acgcccgcat gacgaagtaa
     3361 atcgcgcgac ccgttcagta gttgcggtgt caactgttcc agcagatggg tatcgttaat
     3421 cgccagcacg gtttgatcgt ctcgattggc aattgccaga tacgtcgatg tgctttgacc
     3481 atgcaaacga acgcagccgg agacattcac gccggcacgg cgcgtttctt ccaggagcat
     3541 ttcgccataa aagtcatcgc caatcactga aagcaaatgg acgtcacggc ctaacagcgc
     3601 cagattgtgg gcgatattgc gtccaacgcc gcctgccgaa caatgaattg taccgggatg
     3661 agaagccgct tgcgggtaat ggatatccgc catcccgcga atatccatat tgattgtccc
     3721 caccactacg cagtattcct gctcggtgag aatgtaacct ttgcctttaa tccggccttt
     3781 gcgcattaaa tccataatat gcgccgcaac gcgcgaacga ctgatttgca gcatgtccgc
     3841 aatttcgttc tgctgaatca gcgggttacg ccgtaaaatt gcaaggatct ccttttcccg
     3901 gttattcatc ttcaggctac cgctttttca gtttgttgcg ctttcagcca ggcgatctct
     3961 tctgcccaga tatccgggtt gatggtttca aggatcagcg gaatgccgtc gaaacggtcg
     4021 ttctgcataa tccagcggaa cgcatcatga ccgatattgc cttcaccgag gctatgatgg
     4081 cggtcaacgc ggctgccaaa ggtacttttc gcatcgttaa ggtgcatccc gcgcagatac
     4141 ttaaagccga caatacgggc aaaatcagcg aacgtttttt cgcattcggc tggagtacgc
     4201 aaatcatacc cggcagcgaa agcatggcag gtatcaatgc agacgccgac gcgggattta
     4261 tcttccacgc cgtcgataat cgccgcgaga tgttcgaatt taaaccctaa gttactgccc
     4321 tgaccggcgg tgttttcaat caccgctgtt acaccttgag ttttatccag cgcaatgttg
     4381 atggattcgg caatacgcgc aaggcaatcc tcttctgaaa tctgcatcag atggctgcca
     4441 gggtggaagt tgagcaaaga aagccccagt tgttcgcaac gctgcatttc atctataaag
     4501 gcatcgcgcg atttttccag agcttcagtg accggatgtc cgaggttgat cagataactg
     4561 tcgtggggaa gaatttgcgc cgatgtgtag tgatattttt cacaggcggc tttgaattca
     4621 tcgatggttt gcgtcgtgag cggtgcggca cgccactgac gttggttttt ggtgaacaag
     4681 gcaaacgcgg ttgcgtcgat ttcggcggcg cgaattgcgg catttgccag accgccagca
     4741 gcactaacgt gcgctccaat gtatttcatg cgaggactcc tgttaaaccc gctggaggaa
     4801 aacggtaatg atagcgggtt aacaggagca agaagtagtg gtttatgcga tgacgctttg
     4861 aatcacatag ttaatcgcac caccaccaac aatcagccag gcaaacagta ccagtgccat
     4921 cagcagaggt ttcgccccag cttttttcag cgcgctgacg tgagtggtca gacccagcgc
     4981 cgccatcgcc attgccagca ggaaggtatc cagcgttacc agcatgttca ccacgctctg
     5041 cggtaacagg tggaacgagt taaagatggc aactacgatg aacaagatgg caaaccacgg
     5101 aatagtgatt ttgcttttct cgccgctgtt cgccccagac agctgtttaa cacgcgccgc
     5161 cagcaggatg aggaacggag ccagcatcat cacgcgcagc attttggaaa taactgctgc
     5221 gttttctgca tccgggctga tggcatgacc tgccgccacc acctgcgcca cttcgtgcac
     5281 agtagaacca atgtagatac cgaaagtttc cggactaaac cattgagaca tcagcggata
     5341 tatcgccggg tagaggaaaa tcgcgacggt cccgaagata acaacggttg caacagccac
     5401 ggttacttta ctggcttccg ctttcactac cggctcagtc gccagtaccg cggcagcacc
     5461 acagatactg ctaccggcac cgatcaacca actggtgtgc ttatccagac caaacacttt
     5521 ctgccccagg aagcaagcca gcaggaaggt actggacagc gtcagcacgt caatgatgat
     5581 cccactgata ccgacatcgg caatttgcga gaacgtcaga cggaagccat aaagaatgat
     5641 acccagacgt aataaatatt gcttggcaaa cagcacacca ccgtcacagc ttttccagat
     5701 gtgcggatag atggtgttgc ctaaaaccat ccccaacaag attgcgaggg tgagggcact
     5761 aaacccggca cccgcaaccg cgggaatgga accaccccac agggcgaccc cggtgataac
     5821 tgcactcagg gctaaccccg gaataaaatg ccacagtgta cgatgttgtt tctgtaaggt
     5881 gatattcgtc ataaccctct cctttaaccg ggcataaggt tacgactaac tggtttaaaa
     5941 ataaaattga ttatatattt ataattaatc tttataagtg gtaagcgact atgcacatca
     6001 ccctccggca gttggaagtt tttgcagaag tattgaaaag tggatcaacc acccaggcgt
     6061 cggtgatgct ggcgttgtcg caatcagcag tgagcgcagc cttgactgac ctggaagggc
     6121 agcttggcgt tcaactgttt gatcgcgtgg ggaaaagact ggttgttaat gaacacgggc
     6181 ggctgctcta tccgcgagcg ttggcactgc ttgaacaggc ggttgaaatc gaacaactgt
     6241 ttcgcgaaga caacggcgcg attcgtatct atgccagtag taccatcggt aactacattc
     6301 tgcctgcagt tatcgcccgt tatcgccatg attatccgca gttgccgatt gaacttagcg
     6361 ttgggaacag ccaggacgtg atgcaagcgg tgctggattt ccgcgttgat attggcttta
     6421 ttgaaggacc gtgccacagc actgaaatca tttctgaacc gtggctggaa gacgagctgg
     6481 tggttttcgc cgcgccgact tcgccgttgg ctcgtggtcc ggtcacctta gaacagctgg
     6541 ccgctgcgcc gtggatcctg cgtgaacgcg gttccggcac gcgggagatt gtcgattatc
     6601 tgttgctgtc acatttaccg aagtttgaga tggcgatgga attaggtaac tccgaggcaa
     6661 tcaaacatgc ggtgcgtcat gggttgggaa ttagttgcct gtcgcgacgt gtgattgaag
     6721 atcaattgca ggcaggcaca ttaagtgaag ttgcggtccc tctgccgcgc ctgatgcgta
     6781 cgttgtggcg tatacatcat cggcaaaaac acctttccaa cgcgctacgg cgctttctgg
     6841 actattgcga tcccgcaaat gtgccgcgtt aagttgctgt acaagaacat gctggtgctg
     6901 tgtcgatttc gtgacgcagc gccttcagca tgcattcgcc agaaaagaga ttggctgctt
     6961 tacttataat ccctgggcga tcatgaaggt gtcttataac cgtgtatttc tgccggaagg
     7021 attgccaatc gtctgctaca atcgcgccat catttttaag atggatagca tttttgtatg
     7081 ggttccgaaa ctaaaactac agaagcgccg ggcttacgcc gtgaattaaa ggcgcgtcac
     7141 ctgacgatga ttgccattgg cggttccatc ggtacaggtc tttttgttgc ctctggcgca
     7201 acgatttctc aggcaggtcc gggcggggca ttgctctcgt atatgctgat tggcctgatg
     7261 gtttacttcc tgatgaccag tctcggtgaa ttggctgcat atatgccggt ttccggttcg
     7321 tttgccactt acggtcagaa ctatgttgaa gaaggctttg gcttcgcgct gggctggaac
     7381 tactggtaca actgggcggt gactattgcc gttgacctgg ttgcagcaca gctggtcatg
     7441 agctggtggt tcccggatac accgggctgg atctggagtg cgttgttcct cggcgttatc
     7501 ttcctgttga actacatctc tgttcgcggc tttggtgaag cggaatactg gttctcactg
     7561 atcaaagtca cgacagttat tgtctttatc atcgttggcg tgctgatgat tatcggtatc
     7621 ttcaaaggcg cgcagcctgc gggctggagc aactggacaa tcggcgaagc gccgtttgct
     7681 ggtggttttg cggcgatgat cggcgtagct atgattgtcg gcttctcttt ccagggaacc
     7741 gagctgatcg gtattgctgc aggcgagtcc gaagatccgg cgaaaaacat tccacgcgcg
     7801 gtacgtcagg tattctggcg aatcctgttg ttctatgtgt tagcgatcct gattatcagc
     7861 ctgattattc cgtataccga tccgagcctg ctgcgtaacg atgttaaaga catcagcgtc
     7921 agtccgttca ccctggtgtt ccagcacgcg ggtctgctct ctgcggcggc ggtgatgaac
     7981 gcagttattc tgacggcggt gctgtcagca ggtaactccg gtatgtatgc gtctactcgt
     8041 atgctgtaca ccctggcgtg tgacggtaaa gcgccgcgca ttttcgctaa attgtcgcgt
     8101 ggtggcgtgc cgcgtaatgc gctgtatgcg acgacggtga ttgccggttt gtgcttcctg
     8161 acctccatgt ttggcaacca gacggtatac ctgtggctgc tgaacacctc cgggatgacg
     8221 ggttttatcg cctggctggg gattgccatt agccactatc gcttccgtcg cggttacgta
     8281 ttgcagggac acgacattaa cgatctgccg taccgttcag gtttcttccc actggggccg
     8341 atcttcgcat tcattctgtg tctgattatc actttgggcc agaactacga agcgttcctg
     8401 aaagatacca ttgactgggg cggcgtagcg gcaacgtata ttggtatccc gctgttcctg
     8461 attatttggt tcggctacaa gctgattaaa ggaactcact tcgtacgcta cagcgaaatg
     8521 aagttcccgc agaacgataa gaaataagtt ttctcccttc cttgctaagc cctctcaacc
     8581 gagagggctt tttcaattcc atttccctga caaatcatgc ggatataaaa tttaacattt
     8641 ggattgataa ttgttatcgt ttgcattatc gttacgacgc aatcaaaaaa ggctgacaaa
     8701 tcagaggctg ttccggcttt ctgggatgat cacctgcata caaataagtc caccgcgatg
     8761 ctgccgtacg caaggggacg taaagaagat gtgagcgata cccattttat tttcgtagtt
     8821 acctcatgga gatatggaat gtttaggttg aaccctttcg tacgggtcgg gctgtgtttg
     8881 tccgctattt cttgtgcatg gcctgtgtta gcggtcgatg atgatggcga aacgatggtt
     8941 gtcactgcat cttccgtgga acaaaatctt aaagatgcac ccgccagtat cagcgtcatt
     9001 acccaggaag acctgcagcg aaaaccggta cagaatctga aggatgtcct caaagaagtg
     9061 cctggcgtac aactgacgaa cgaaggggat aaccgtaagg gcgttagtat tcgtggtctg
     9121 gacagcagct acaccctgat tcttgtcgac ggtaaacgcg ttaactcccg caatgccgtc
     9181 ttccgccaca atgatttcga tctgaactgg atcccggtcg attccatcga acgtattgaa
     9241 gtggttcgtg gcccgatgtc gtcgctatac ggctccgatg cgctcggcgg tgtagtgaat
     9301 atcatcacca aaaaaatcgg tcagaaatgg tcgggtaccg ttaccgtcga taccaccatt
     9361 caggaacatc gcgatcgcgg tgatacctat aacggtcagt tctttaccag tggaccatta
     9421 attgatggtg tgctgggaat gaaagcttac ggcagcctgg caaaacgtga aaaggatgac
     9481 ccgcaaaact caacgaccac cgataccgga gaaacgccgc gtattgaagg attctccagc
     9541 cgcgacggca atgtcgaatt tgcctggaca ccgaatcaaa atcacgattt tactgccgga
     9601 tacggtttcg accgtcagga tcgtgattcc gactcgctgg acaaaaaccg cctggaacgc
     9661 cagaactact gcgtcagcca taatgggcgt tgggattacg gcaccagcga actgaaatcc
     9721 tacggtgaga aagtcgagaa caaaaaccct ggcaacagca gcccgataac ttccgaaagc
     9781 aatacggtcg acggcaaata cacgttgccg ctgacggcga ttaatcagtt tctcacggtt
     9841 ggcggtgaat ggcgtcacga caaacttagc gatgcggtga acctgaccgg gggaaccagc
     9901 tccaaaacgt ctgccagcca gtacgcgctg tttgtggaag atgaatggcg gatcttcgag
     9961 ccgctggcgc tgacgaccgg cgtgcgtatg gacgatcacg aaacctacgg tgaacactgg
    10021 agtccgcgtg cctacctggt ttataacgcc accgacaccg taacggtgaa agggggctgg
    10081 gcgacggcat ttaaagcacc ttctctgttg caacttagcc ctgactggac gagcaattcc
    10141 tgccgtggcg catgtaagat tgtgggtagc ccggatctga aaccagaaac cagcgaaagt
    10201 tgggagctgg ggctttacta catgggtgaa gaaggctggc tggaaggggg tgaatccagc
    10261 gttatcgttt tccgtaacga tgtgaaagat cgtatcagca tcagccgtac gtctgacgtc
    10321 aacgctgcac cgggctacca aaactttgtt ggttttgaga cgggcgctaa cggacggcgc
    10381 ataccggtat ttagctacta cagcgttaac aaagctcgta ttcagggcgt ggaaaccgaa
    10441 ctgaaaattc cgttcaacga tgaatggaaa ctgtcgatca actacaccta caacgatggt
    10501 cgtgatgtca gcaacggcga aaacaaaccg ctatccgatc tgccgttcca tactgctaac
    10561 ggtacgctgg actggaaacc gctggcgctg gaagactggt cattctatgt ttctggtcac
    10621 tataccgggc agaaacgcgc cgacagcgcg acggctaaaa caccgggcgg ttataccatc
    10681 tggaataccg gcgcggcctg gcaggtgact aaagacgtca aactgcgcgc aggcgtgctg
    10741 aaccttggcg acaaggatct cagtcgtgac gactacagct ataacgaaga cggacgtcgt
    10801 tactttatgg cagtggatta tcgcttctga tgagaagatg cccggcgaac cgggcggact
    10861 ttcacttcag taaatactgc gcatggaagc gcaggtgatc ctctataaaa gaggcgatga
    10921 agtagtaact gtgatcatat cccggctgaa tacgcagcgt catcggccac gctttctggc
    10981 gcgcggcttc tgccaatacc gcaggttgca actggtcggc aagaaactga tcattatcgc
    11041 cctgatcgat aagcgtcggg atcgcatcct gcgcgttact ggcatacatc agcgcgcaac
    11101 tatcccattc cagccatgca tttttgtcct cacctaaata ggtgctaaac gctttgattc
    11161 cccacggggc gctgcacgga ttcacaatgg gcgcaaaggc cgaaacgctg gtgtatttac
    11221 ccggattttt cagcgccata atcagcgcac cgtgaccacc cattgagtga ccgctaatgg
    11281 cgcagcggtc gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat
    11341 aatcatacat ccggtaatac gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg
    11401 cgccctggcc taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt
    11461 ctggcatcac cagtacaatc cccagttccg ccgctacccg ctgggcaccc gccttggtgg
    11521 tgaagttctc gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt
    11581 gatcacgagg gggtgggaga aagatactga acgtcatcgg gcagtttaag gtactggagt
    11641 cgtgtcgcca gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc atttccatgc
    11701 gtggctcctt gttgtgttgt ttgcaatttc ttcatcatac cgtttattca atgtgctgtg
    11761 agtaactttc acttccgtat ttgcataacg atgttttaac atctgctgat gaaaggcagc
    11821 ggcaattaca ataattatcg ctgtgaatac tggattatgt gcgccgcctc acgcacaata
    11881 atcaagctgt aaatcagctt aataactttg cccccacgca gggcggaggc gtcacacctg
    11941 caggagaaat cataaatgcc atcactcagt aaagaagcgg ccctggttca tgaagcgtta
    12001 gttgcgagag gactggaaac accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg
    12061 cgcaaaagcc ttattgctgg tcatatgacc gaaatcatgc agctgctgaa tctcgaccta
    12121 gctgatgaca gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt
    12181 ttctccggtc tggattacgc caatttcccg aaaatcaccc tcattgaaaa caaaatgaag
    12241 gtcgatgaaa tggtcaccgt gcgcgatatc actctgacca gtacctgtga acaccatttt
    12301 gttaccatcg atggcaaagc gacggtggct tatatcccga aagattcggt gatcggtctg
    12361 tcaaaaatta accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg
    12421 acgcagcaaa ttcttattgc gctacaaacg ctgctgggca ccaataacgt ggctgtctcg
    12481 atcgacgcgg tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccacg
    12541 acaacgacct ctcttggtgg attgttcaaa tccagtcaga atacgcgcca cgagtttctg
    12601 cgcgctgtgc gccatcacaa ctgattaaaa ggcaggaacc atggagcgca acgtcacgct
    12661 cgattttgtt cgcggcgtcg ccattctggg gatcctgcta ttaaacatca gcgcctttgg
    12721 gctaccaaag gcggcttatc tcaatcccgc ctggtacgat gctattacgc cgcaggatgc
    12781 atggacctgg gcatttctcg atctcatcgg ccaggtgaaa ttcctcacgc tttttgcgct
    12841 gctgtttggt gctggcctgc aaatgttgct gccccgtggc agacgctgga tccagtcacg
    12901 gttaacgctg ttagtcttgt tgggctttat tcacggttta ttgttctggg acggcgatat
    12961 tctgctggct tacgggctgg tgggcttaat ctgctggtgg ctggtgcgcg atgcgccatc
    13021 ggtaaaaagc ctgtttaata ccggcgtcat gctttatctg gtggggcttg gcgttttgct
    13081 gttattgggg ctgatttccg acagccagac cagccgcgcc tggacgccgg atgcatcggc
    13141 tattttgtat gaaaaatact ggaagcttca cggcggcgtt gaagcgatca gtaatcgtgc
    13201 cgatggtgtt ggcaacagtt tactggcact gggcgcacag tatggctggc aactggcagg
    13261 gatgatgctc attggtgcgg cgctgatgcg cagcggctgg ctgaaagggc agttcagctt
    13321 acgtcactat cgtcgtactg gttttgtgct ggtggcgatt ggggtgacca ttaaccttcc
    13381 tgccatcgcc ctgcaatggc agctggactg ggcgtatcgc tggtgtgcct tcttacttca
    13441 aatgccgcgg gaactgagtg cgccgtttca ggcgattggc tatgcgtcgc tgttttatgg
    13501 cttctggccg caattgagcc gctttaagct ggtgcttgcg atcgcctgcg tcggacggat
    13561 ggcgctgacc aactatctat tgcaaacgct gatttgtacc acgctttttt accacctcgg
    13621 tttgtttatg cattttgacc gcctggagct gctggcgttt gttattccgg tatggctggc
    13681 gaatatcctc ttctctgtta tctggctgcg ttacttccgc caggggccgg tggaatggct
    13741 ctggcgtcag ttaactttgc gtgctgccgg actggcaata tctaaaacat caagataacg
    13801 atctggatca cattcgttaa caaaacggct gtaaccgttt ccattgctgt gactcgattc
    13861 acgaagtcct gtattcagtg ctgacaaaat agccgccagc aagcagtcat ttactgcaat
    13921 ctcataacag gtagtgaata tgatcaccat tcgtgatgta gcgcgtcagg ctggcgtctc
    13981 tgtggcaacg gtttcccggg tgctcaataa cagcacgctg gtcagtgccg acacgcgtga
    14041 agcagtaatg aaagccgtga gtgagctgga ttatcggcca aacaccaatg cccaggcgct
    14101 ggcaactcag gttagcgaca ccattggcgt ggtggtgatg gacgtttctg atgcgttttt
    14161 cggcgcgctg gtaaaagcgg tggatctggt cgcccagcag catcagaaat acgtgctaat
    14221 cggcaatagc tatcatgaag cggaaaaaga gcgtcacgcc attgaggtgt taattcgcca
    14281 gcgttgtaat gcgttgattg ttcactcaaa agcattgagt gacgatgaac tggcacaatt
    14341 tatggataac attcccggta tggtgttaat caaccgcgtt gtgccggggt acgcccatcg
    14401 ttgcgtttgc ctggataatc tcagcggtgc ccgaatggcg acgcgcatgt tgctgaataa
    14461 cggtcatcaa cgtattggtt atctttcttc cagccacggc attgaagatg acgccatgcg
    14521 taaagcaggc tggatgagtg cgttgaaaga gcaggatatt attccgccgg aaagctggat
    14581 tggcactggt acgccggaca tgccgggcgg tgaggcggcg atggttgaac tgctggggcg
    14641 caatctacaa cttaccgctg tatttgctta taacgacaat atggccgctg gcgcactgac
    14701 agcattaaaa gataatggca ttgcgattcc gttacatctc tcaatcatcg gtttcgatga
    14761 tattcccatc gcccgttaca ccgacccgca attaacgacc gtgcgttatc ccattgcttc
    14821 aatggctaaa ttagccaccg aactggcctt gcagggggca gcaggcaata ttgatcctcg
    14881 tgccgccact gttttatgcc gacgttagtg cgtcgccatt ctgtcgcaac gcgccagaat
    14941 gcggcggcga tcactaactc aacaaatcag gcgatgtaac cgctttcaat ctgtgagtga
    15001 tttcacagta tcttaacaat gtgatagcta tgattgcacc gttttaatgt tgtaacccgt
    15061 atgtaacagt gaataatcac tttcaccgag gtaatagcgt cataacaaca attaaagccg
    15121 ttttctggag cgttaccggg catggaagaa cgaattttaa aaagtgagct tcggcgttca
    15181 gtaacacttc attaactcta ctgccccgcc gagcatttat ctcaagcact accctgcata
    15241 agaaaaaccg gagataccat gaataagaag gtgttaaccc tgtctgctgt gatggccagc
    15301 atgttattcg gtgccgctgc acacgctgct gatactcgca ttggtgtaac aatctataag
    15361 tacgacgata actttatgtc tgtagtgcgc aaggctattg agcaagatgc gaaagccgcg
    15421 ccagatgttc agctgctgat gaatgattct cagaatgacc agtccaagca gaacgatcag
    15481 atcgacgtat tgctggcgaa aggggtgaag gcactggcaa tcaacctggt tgacccggca
    15541 gctgcgggta cggtgattga gaaagcgcgt gggcaaaacg tgccggtggt tttcttcaac
    15601 aaagaaccgt ctcgtaaggc gctggatagc tacgacaaag cctactacgt tggcactgac
    15661 tccaaagagt ccggcattat tcagggcgat ttgattgcta aacactgggc ggcgaatcag
    15721 ggttgggatc tgaacaaaga cggtcagatt cagttcgtac tgctgaaagg tgaaccgggc
    15781 catccggatg cagaagcacg taccacttac gtgattaaag aattgaacga taaaggcatc
    15841 aaaactgaac agttacagtt agataccgca atgtgggaca ccgctcaggc gaaagataag
    15901 atggacgcct ggctgtctgg cccgaacgcc aacaaaatcg aagtggttat cgccaacaac
    15961 gatgcgatgg caatgggcgc ggtagaagca ctgaaagcac acaacaagtc cagcattccg
    16021 gtgtttggcg tcgatgcgct gccagaagcg ctggcgctgg tgaaatccgg tgcactggcg
    16081 ggcacagtac tgaacgatgc taacaaccag gcgaaagcga cctttgatct ggcgaaaaac
    16141 ctggccgatg gcaaaggtgc ggctgatggc accaactgga aaatcgacaa caaagtggtc
    16201 cgcgtacctt atgttggcgt agataaagac aacttggctg aattcagcaa gaaataagac
    16261 tgattgggcg cagtcgttta ctgcgccctt attgtatggc caacaaggta aaattatatg
    16321 gtcagctcaa cgactccgtc ttccggggaa tacttgttgg aaatgagcgg tatcaacaag
    16381 tcctttcctg gtgttaaggc acttgataac gttaatttaa aagtccggcc acattctatc
    16441 catgcattaa tgggggaaaa cggtgcagga aaatcgacat tattaaaatg cctgtttggt
    16501 atttatcaaa aagactccgg caccatttta ttccagggta aagagatcga tttccattcc
    16561 gccaaagagg cactggaaaa tggtatttcg atggtacacc aggagttaaa cctggtatta
    16621 caacgttcgg tgatggataa catgtggctg gggcgatatc ccaccaaagg catgtttgtc
    16681 gatcaggaca aaatgtaccg cgaaaccaaa gcgatttttg atgaactgga tattgatatc
    16741 gatccgcgtg cgcgcgtcgg cacattatcc gtttcgcaaa tgcagatgat cgaaatcgcc
    16801 aaagcgtttt cctataacgc gaaaattgtg attatggatg aaccgacttc ttcgttaacc
    16861 gaaaaagagg tcaatcatct gttcactatt attcgtaaat taaaagagcg cggctgcggt
    16921 attgtttata tctcgcataa aatggaagaa atcttccagt tatgtgatga agttaccgta
    16981 ttgcgcgacg gtcagtggat cgccaccgaa ccgctggcag gactgacgat ggacaagatc
    17041 atcggcatga tggttgggcg ttctcttaat cagcgttttc ctgacaaaga aaacaagccg
    17101 ggcgaagtca tcctcgaggt gcgtaaccta acgtcactgc gccagccgtc gattcgcgat
    17161 gtctcgtttg atctgcataa aggggagatc ctcggtattg ccgggctggt gggggcgaaa
    17221 cgtaccgata ttgttgagac gttatttggt attcgcgaga aatcggctgg caccattacg
    17281 ttgcacggca aacagatcaa taaccataat gccaacgaag ccataaacca cggatttgca
    17341 ctggtaactg aggagcgccg ctcaacggga atttatgcct atctggatat tggttttaac
    17401 tcgttaattt ccaatattcg caactacaaa aataaagttg gtttactgga taactcgcgg
    17461 atgaaaagcg atacccagtg ggtgattgat tcgatgcggg taaaaacgcc gggtcatcgg
    17521 acgcaaattg gttcgctctc cggtggtaat cagcaaaagg tgattattgg tcgctggcta
    17581 ctaacgcaac cagaaatatt aatgctcgat gaaccgacgc gcggtattga tgtcggggcg
    17641 aagtttgaaa tttatcagtt aattgccgaa ctggcgaaga aaggcaaggg gattattatt
    17701 atctcctctg aaatgcctga gttgttaggg ataacagacc gtattctggt catgagcaat
    17761 ggtctcgttt ccggaattgt cgatacaaaa acaacaacgc aaaacgaaat tctgcgtctt
    17821 gcgtctttgc acctttaaga ttaggggctt cccatgagtg cgttaaataa gaaaagtttt
    17881 cttacttacc tgaaagaggg cggtatttac gtcgttcttt tagttttgct ggcgattatt
    17941 attttccagg acccaacatt tttaagtctg ttgaacttaa gtaatattct cacccagtca
    18001 tcggtgcgta ttattatcgc gctcggtgtg gcagggttaa ttgtcaccca ggggaccgat
    18061 ctttctgctg gtcgtcaggt agggctggcg gcagtggtgg ctgcgacatt attgcagtcc
    18121 atggataacg ccaacaaagt gttcccggaa atggcgacga tgccgattgc gctggttatt
    18181 ctgattgtct gtgccattgg tgcggtgatc ggtttgatca acggtctgat tatcgcttat
    18241 ctcaacgtga cgccgttcat taccacgctc ggcacgatga tcatcgtcta tggcatcaac
    18301 tcgctctatt acgactttgt cggggcgtcg ccaatttctg gttttgacag tggcttctct
    18361 acctttgctc agggctttgt cgcgctgggg agtttccgtc tctcttacat caccttctac
    18421 gcgttgattg cggtggcgtt cgtctgggtg ttgtggaaca aaacccgctt cggtaagaac
    18481 atttttgcca ttggcggtaa cccggaagcg gcaaaagtat ctggtgtcaa cgtcggcctg
    18541 aacctgctga tgatctacgc gttgtctggc gtgttctatg cctttggcgg gatgttagaa
    18601 gccggacgta tcggctctgc caccaacaac ctcggcttta tgtatgagct ggatgctatc
    18661 gcggcgtgcg tggtaggcgg tgtatcgttc agcggcggtg tggggacggt gattggcgtg
    18721 gtgaccgggg taattatttt taccgtcatc aactatggcc tgacgtatat cggcgtaaac
    18781 ccatactggc agtacatcat caaaggggcg attattatct tcgccgtagc gctggattca
    18841 ctgaaatacg cgcgtaagaa gtgatgtgat tgcggatatt gccggatgcg tctctggttt
    18901 tacaacgtta ccgggtgttc tttctcgcct ttcttaaact tcacttcccc gagctcaata
    18961 caacccaccg ggcagacgtg accacaaagc agacaaccca cacatttctc ggtattacaa
    19021 tgcggtgtgc gggttttctc gctccattcc atcgcctggt gaccgccgtc gtaacaggaa
    19081 atataacagc gtccacagcc aacacatttg tcaagattga tacggggata gacaatataa
    19141 ctgcggtcta aatcttccgc cgggacaata ttgttattcg ccagacctac catctcctgc
    19201 aacgaatcaa atccctgatc ggcgagataa tgcgataacc cgctcgccat atcttctacg
    19261 atgcgatagc cgtactgcat aatgccggtg gtgacctgta acgttgctgc gccgagcaat
    19321 aaaaactccg ccgcatcttc ccaggtttca atgccgccga taccgctgat tgggaaatcg
    19381 cgcagttctg gatgggtgcg catttgctga atgaagcgca gggcgatcgg ttttaccgct
    19441 ttaccggaat atccggaaat actcgattta ccgttaacga tcggcatacc gattttctga
    19501 ttaagatcga tattggtgat ggatttaacg gtgttaattg ccgcaatgcc atctgcgccg
    19561 ccgcgcttcg ccgccagcgc cacttcgcac atatcaccga tattcggcgt catcttcgcc
    19621 agcattggca gcgtggaacc ccgtttcact gcccgacaat atttttttac cagctccggg
    19681 ctttgcccga catcgctacc catcgcatga gaagtcattt gcggacagga gaagttacac
    19741 tcgatcatat ccgcgccagc ttcttgcacc aggcgcgcca gctcctccca ttgctgctca
    19801 ttttccccca tgatcgaagc gatcaatact ttgtccgggt aatcttcctt cagccgacgc
    19861 agggcggtca gattctcttc caacggatgt tcagcaatct gctccatatt tttgaagccg
    19921 ataaaaccgg tatcttcttt caccagatga tcaaaacgcg gcgagacttc gttggcgata
    19981 aaaaagccga tcgttttaaa caccacaccg ccccaacctg tgtcgtaggc tttggcacac
    20041 atctcatagc agttgcctac cggcgaagaa gagaggcaga acgggttggg aaagttcacg
    20101 ccgcaaaaag taatcgaaag atctttcgtt aacatgagca agctccctct aatatagtga
    20161 tgaatcgcct cggcggcttc tttcccggtt ttcacggcat agaccacggt tttgtcaccc
    20221 tcaacaatat cgccagcagc aaagacttgc ggatcgcggg tctgataatt ttgtgttttg
    20281 atggtgttac gctgctgctc taactccgca aaggcatcca gtctggcatg ctgaccgacg
    20341 gcgagaataa ttttatctgc cgccatcgtc agttcgcctg ataaccgtac atgcttaaac
    20401 gtgactttat tgccttccac ggctactggc gtgaatccat cgatgatcga aacgcccagc
    20461 tcccgggcgc tggcaaactc tttttcgctt gccggaaact catctaactc ttcacgtgct
    20521 acgcaagtta ccgcctgaca gccgagaact ttcagcgtgc tggctacgtc catcgcgaca
    20581 tcaccgccgc cgataattaa tgcgctttgt ggaatgctga tatcgccttg cgcctgtcgt
    20641 acacgttgca ggaagtcgac ggcaatctca acgtcgctat gctcaaacag cggtagcccg
    20701 gaaccgcttg ataaccccac ggtgaccagt accgcgcggt tttccgcttt cagttgctcc
    20761 agggtgagtg ttttaccgat ttcgttattg cacttaatgg tcacgcccat tttttcaata
    20821 cgggcgatct ctgcatccag cactgattgc ggtaaccgga attgcggaat accgttacgc
    20881 aaccagccac cggggtgcgc ttctttctca taaatcgtga cgtcataacc ctggtttgtc
    20941 agtgtcacac tggcctgcaa tccggcagga cctgcgccaa taatcgcgac tttgccgagc
    21001 gttttagtac cgggctgata aatttccatc ccggtttgtt gttcaaaatc agtaacaaaa
    21061 cgctgtaagc ggccgatatc aatgggagca tcaacaccgg cacgggtaca accgctttgg
    21121 cataatttct ccgtcgggca cactctggca caaacggcac cgagggcatt attttcgcga
    21181 attgtctcgg cagcgccttt aaaattacga aagtagattg agcgaataaa tttccccgga
    21241 tcggtctgtg ccgggcaagc ctgactacag ggagcgtcgt gacataataa acagcgagag
    21301 gcttctttaa tcgccagtaa cggcgtaaaa gccggagtga gttcatccag ataattttgt
    21361 tgcggcatgt gatcttcctt aatgctatga gtaattatgg cgaacgctaa ataaatattg
    21421 cgtgctgata ttaaggctat gcgcgtgggg aaatttaaac tgtgaaagga ttcacaaata
    21481 gctgcggttc agaatgttat atccgcagct tattgagtga agtaataatt attctcaata
    21541 attaacagaa aagagaaaat attaacgata gaaaataaga taaacaatca tgccgataga
    21601 taaaattccg aagccaatat atgccaccat ataagcgaag aagagtccgg gattttttgc
    21661 tcgccagaca tacaccggtt ccggtgcatt tttaacccgt ttatcaatcg cgcctgcgcg
    21721 ccagcctttc catgcttcgc ggaaacaaaa tatcgggata aggaaaaaaa cggcaacgac
    21781 aaaaaactgc tgtacatcca taactacttt ccttgtttct tttgtaattc aagtaactgt
    21841 tcgggtgtaa cggcgggata cccctgcgca tcttccggca cctggtgcat agccggaata
    21901 gggaccagcg gccctaaaaa acgcggttca cgtttaaaaa tataaaggtc agccagcgca
    21961 ccgaaacggg cggcaaattc gcgaatacgt actgacagca tatctttcgg tgacggtacg
    22021 gcataacact gagcctgaat ccccatatgc agcgcaataa ataatgctcg ctcacagtgg
    22081 aaacgttggg tgataataat gaaatcatta gtatcgaaaa ctttgcgtgt acgcacgatg
    22141 gaatccagcg tacgaaagcc tgcgtaatcg agaacaatat ctgatgggtc gacaccagca
    22201 gcgattaaat ctttgcgcat ggtcatcggc tcgttataac tttgcaatgc gttatcgccg
    22261 ctcagtaata gataatttac cttaccgctg ttataggcat taatcgctcc ttgaatgcgg
    22321 tagcgataat actgattaat aacgccggta cgataatatt ttgctgtgcc gagcaccacg
    22381 ccgacctggc ggtaggggag atcctgcaat tcgtcgtaga tataaggcgc ggttttccag
    22441 ctcatccagc gatcgaggcc gagcacagtc aacagcagca agccgatcag gactaacagg
    22501 ctgaggaaca cgcgctttaa catgcaggag actcgagcta aggaaaattt ccatcaggct
    22561 actttacccg ctggttttgc gcaagaaacg gcaaactgtt tgccgggaat ttcgacacca
    22621 ggagagggct ggcaagccgg acaaggcgtt cacgccgcat ccggcaccag gcttaagcga
    22681 gaagcacgcg gtcgatactg tgacagccga gagctttcag cgttgcggag gtggcatccc
    22741 actgaatcaa cggcgcatcg gctttttctg ccagaaccgc gcgctggata tccgggtaat
    22801 cataaccctt gagattcaac agaattaacg ccccctgcaa cggcggcaga gtcgggttga
    22861 atgcggcgtt ttcagcgtag ctgccgctga aaatacgacc gtctttacat tccagcgcga
    22921 caccgcttgg tgacttactg taaggcatgt gcgaacggtt tgccgctgca atcgccgcct
    22981 gagaaagcgc atcaccagtc agcgcatagc cgtgatcctg ctcgtccatc agcagcgttt
    23041 taatctccag atctttcggc ccaaaggcat ccggcagata gtcacgcagc gcgtgtgcct
    23101 cgcggcccgg caaatgaata cgcagatcca gaccgctgtt cagttcattc ataaactgac
    23161 ggcagtgacc acaaggcgta tagttaacgg tgatggctgc aagcgctttt tcaccactca
    23221 accaggcgtg gctgatcgcg ctttgttcgg catgaacggt ttgctgcatt gttgcaccaa
    23281 taaattccat attggcacca aaataccagg ttccgctcac accgcgcgca attgcgccaa
    23341 cattaaaatt cgacaatggc gtacgcgcac aggccgccgc cagcggaagt agtgcgaatg
    23401 ccagcgcgtc ttcgtccagc cccgttgcgc tcttcagcga tgagacttgc tccccggtca
    23461 acaaagcggg gaagtacttg tctgccagaa taggttccag tgcagattgc aagttatccg
    23521 caagttgggc aaaagcggtt tgaaaacgtg gatgcatggc gttgcctcat aatcatgtaa
    23581 tgggataaca gtttacgaac ccgttatggc tttatatgtg atctgaatct cattaattat
    23641 gcaacacgtg aaacttacat caaaaatgcg cgacgtatcg caaattttag cccattactg
    23701 ccagaataat cgggaaaagg aacggtgcga tcagcgaagt aattatcccg cataacacca
    23761 gcgctagcga actaaatgca ccttcctgat aatccagctc ggcgcagcgc gccgtaccga
    23821 gggcgtgcga ggcagtcccc attgccagac cgcgcgcaac tttggtacga atacgcatcg
    23881 cattaagcaa tgtatggcca aataccgcgc cgaggatgcc gacgaaaatc acgcaaactg
    23941 cgctgattgc cggaataccg ccaatactgc cgccaaccga cattgcaata ggcgtggtga
    24001 ctgatttcgg caggattgac gcggcgattt ccggtgaagc gcccatcaac aatgccacgg
    24061 aagtccccgt caccatcgca accacgctgc cgataaaaca gatggtgatg atcgatttcc
    24121 agcgcgcgcg gatctggtgt agctgctcat ataaaggata ggccagcgcg acgaccgccg
    24181 gttgcagcag atcgtttagc acttcgctac ctttaaagta actgtcgtaa gagatgccgg
    24241 tcagcattaa aaaaggaatg atcaccacca ttgctaccag caacgggtta agcaggggaa
    24301 acttataccg tgccgccagt ttgcgggcgg caaaaaagac aatcaacgtc agcggtaatg
    24361 accaccagat attcgccatc attcttctga tcctttctga cctacgactt tacgttcacc
    24421 gtgtaccagt tgtgaactcc agctcaccac cagaaaaacc accagcgtac tgacagcaca
    24481 ggaaaccact accgggccaa actgcgcgcg gagcaaatca aaatattgca tgacgcctac
    24541 gccaatcggc acaaacaata gcgccatata gcgaatcagt acgtagcacc ccggattgac
    24601 ccatttcgcc ggaagaattt gcaaggccag caggacaaac aggatcagca tcccgatgat
    24661 gctgcccgga atggttaccg gtagcaggga agcaatgaaa atgcctgcat acaggcaggc
    24721 ataaatcagg acgaaagcgc gtaaatattg ccagataatg ttcagtgtct tactcataat
    24781 caacacccca gatgagatgg attcatcata caattaattc gtaaaacgtg ctaccgatca
    24841 cattaagaaa tcagcgctta ttttttcgct acccggtatt acgcaatgag cagcgaactc
    24901 aggcatacat caagcataga gagggattgc agtttgcagc aatccctctt tgcagacctt
    24961 taatccgctg accataggcg ctaacatagc gcctcatttt tttgcgggtg atgatatacg
    25021 tgtgttactg gcaccgatgg agggagtgct tgactctctg gtgcgtgaat tgctgaccga
    25081 agttaacgac tacgatctgt gcatcaccga gtttgtccgc gtggtggatc aactgctgcc
    25141 ggtaaaagtc tttcatcgca tttgccctga gctacaaaac gccagccgga caccatctgg
    25201 tacgctggtg cgcgtgcagt tgttaggtca gttcccacaa tggctggcag agaacgccgc
    25261 ccgtgcggtg gagttaggtt cctggggcgt ggatctcaat tgcggctgcc cgtcgaaaac
    25321 ggttaacggt agcggcggcg gggcgacgtt actcaaagat cctgaactca tctaccaggg
    25381 ggcaaaagcg atgcgtgaag ctgtaccggc gcatttgcct gtcagcgtga aagtgcgtct
    25441 gggctgggac agcggtgaga agaaatttga aatcgccgat gcggttcaac aggctggcgc
    25501 tacggagctg gtggtgcatg ggcggacgaa agagcagggt taccgcgcgg agcatattga
    25561 ctggcaggcg attggcgaga ttcgccagcg gctgaatatt ccggtgattg ccaacggtga
    25621 aatctgggac tggcagagcg cgcaacaatg catggcgatc agcggctgcg acgcagtgat
    25681 gattggtcgc ggggcgctca atattcccaa cctgagccgg gtggtaaaat ataacgaacc
    25741 gcgaatgccg tggccggagg tggttgcttt gctgcaaaaa tatacccgtc tggaaaagca
    25801 gggcgatacc gggttatatc acgttgcgcg gattaaacag tggttgagtt atttgcgtaa
    25861 agaatacgat gaagcaacgg aattatttca gcatgttcg
//
LOCUS       DADMEQ010000050        25481 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.050, whole genome shotgun sequence.
ACCESSION   DADMEQ010000050 DADMEQ010000000
VERSION     DADMEQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.050"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            64..522
                     /locus_tag="JNZ05_002092"
     CDS             64..522
                     /locus_tag="JNZ05_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005083197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY8565328.1"
                     /translation="MVNYAFDYLRSPGSLPLTTAATELSAIHGHSTSQYRLGEFYLHG
                     SDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKPDTRKAILWYKEAAEQG
                     YAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(532..1959)
                     /locus_tag="JNZ05_002093"
     CDS             complement(532..1959)
                     /locus_tag="JNZ05_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HAY8565329.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1956..2663)
                     /locus_tag="JNZ05_002094"
     CDS             complement(1956..2663)
                     /locus_tag="JNZ05_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY8565330.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWILSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2821..3804
                     /locus_tag="JNZ05_002095"
     CDS             2821..3804
                     /locus_tag="JNZ05_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HAY8565331.1"
                     /translation="MIIIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAID
                     SPDDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFA
                     SNALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFW
                     YKQAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNA
                     GEGVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECN
                     DATAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(3874..4356)
                     /locus_tag="JNZ05_002096"
     CDS             complement(3874..4356)
                     /locus_tag="JNZ05_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HAY8565332.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTELDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            4591..7173
                     /gene="leuS"
                     /locus_tag="JNZ05_002097"
     CDS             4591..7173
                     /gene="leuS"
                     /locus_tag="JNZ05_002097"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAY8565333.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATLEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7188..7769
                     /gene="lptE"
                     /locus_tag="JNZ05_002098"
     CDS             7188..7769
                     /gene="lptE"
                     /locus_tag="JNZ05_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HAY8565334.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            7769..8800
                     /gene="holA"
                     /locus_tag="JNZ05_002099"
     CDS             7769..8800
                     /gene="holA"
                     /locus_tag="JNZ05_002099"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAY8565335.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8802..9443
                     /gene="nadD"
                     /locus_tag="JNZ05_002100"
     CDS             8802..9443
                     /gene="nadD"
                     /locus_tag="JNZ05_002100"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAY8565336.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9467..10078
                     /locus_tag="JNZ05_002101"
     CDS             9467..10078
                     /locus_tag="JNZ05_002101"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HAY8565337.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10338..10655
                     /gene="rsfS"
                     /locus_tag="JNZ05_002102"
                     /gene_synonym="rsfA"
     CDS             10338..10655
                     /gene="rsfS"
                     /locus_tag="JNZ05_002102"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAY8565338.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10659..11126
                     /gene="rlmH"
                     /locus_tag="JNZ05_002103"
     CDS             10659..11126
                     /gene="rlmH"
                     /locus_tag="JNZ05_002103"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAY8565339.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVILDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11157..13058
                     /gene="mrdA"
                     /locus_tag="JNZ05_002104"
     CDS             11157..13058
                     /gene="mrdA"
                     /locus_tag="JNZ05_002104"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HAY8565340.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWLQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13061..14173
                     /gene="mrdB"
                     /locus_tag="JNZ05_002105"
                     /gene_synonym="rodA"
     CDS             13061..14173
                     /gene="mrdB"
                     /locus_tag="JNZ05_002105"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HAY8565341.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14184..15272
                     /gene="rlpA"
                     /locus_tag="JNZ05_002106"
     CDS             14184..15272
                     /gene="rlpA"
                     /locus_tag="JNZ05_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HAY8565342.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAQTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15411..16622
                     /gene="dacA"
                     /locus_tag="JNZ05_002107"
     CDS             15411..16622
                     /gene="dacA"
                     /locus_tag="JNZ05_002107"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HAY8565343.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16733..16996
                     /locus_tag="JNZ05_002108"
     CDS             16733..16996
                     /locus_tag="JNZ05_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HAY8565344.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17097..17738
                     /gene="lipB"
                     /locus_tag="JNZ05_002109"
     CDS             17097..17738
                     /gene="lipB"
                     /locus_tag="JNZ05_002109"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HAY8565345.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17997..18950
                     /locus_tag="JNZ05_002110"
     CDS             17997..18950
                     /locus_tag="JNZ05_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAY8565346.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQSVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCRP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19159..20124
                     /gene="lipA"
                     /locus_tag="JNZ05_002111"
     CDS             19159..20124
                     /gene="lipA"
                     /locus_tag="JNZ05_002111"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HAY8565347.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20225..20428)
                     /gene="tatE"
                     /locus_tag="JNZ05_002112"
     CDS             complement(20225..20428)
                     /gene="tatE"
                     /locus_tag="JNZ05_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HAY8565348.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20557..21345)
                     /locus_tag="JNZ05_002113"
     CDS             complement(20557..21345)
                     /locus_tag="JNZ05_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HAY8565349.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            21438..21821
                     /gene="crcB"
                     /locus_tag="JNZ05_002114"
     CDS             21438..21821
                     /gene="crcB"
                     /locus_tag="JNZ05_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HAY8565350.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21875..22084)
                     /gene="cspE"
                     /locus_tag="JNZ05_002115"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21875..22084)
                     /gene="cspE"
                     /locus_tag="JNZ05_002115"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY8565351.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22259..22819)
                     /locus_tag="JNZ05_002116"
     CDS             complement(22259..22819)
                     /locus_tag="JNZ05_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid:lipid A palmitoyltransferase"
                     /protein_id="HAY8565352.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23404..24789
                     /gene="dcuC"
                     /locus_tag="JNZ05_002117"
     CDS             23404..24789
                     /gene="dcuC"
                     /locus_tag="JNZ05_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY8565353.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24830..25171)
                     /locus_tag="JNZ05_002118"
                     /pseudo
     CDS             complement(24830..25171)
                     /locus_tag="JNZ05_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT         6521 a         6332 c         6261 g         6367 t
ORIGIN      
        1 tttatagttg cagcaattac ctgtattagc caacccaaga aaacgacgtt gcgtgataaa
       61 gccatggtga attatgcctt tgattattta agatcaccag gcagtctacc gttaaccacg
      121 gcagccacgg agctttccgc gattcatggt cactcaacat cgcaatatcg ccttggcgaa
      181 ttttatcttc atggtagcga cggtaaacca ctggattata cacaggcgag atactggtat
      241 gagcaatcag cagaacagga aaatccgcgc gcacaaagca aactggggtg gatctacctg
      301 aaaggcctgg gagtcaaacc cgatacccgt aaagcaattc tctggtataa ggaagcagct
      361 gaacaagggt atgctcatgc tcaatatact ttgggtttga tctacagaaa tggcactggt
      421 attaatgtta accattatga atctcaaaaa tggttaaaac tggccgccaa acaacattac
      481 aaaaatgcgg aaagattact tgccggactt cccgcacatt aacgtcgact gtcacccgaa
      541 gagatggagt attttcccta tcaaaccggc aaagataaaa atgtagaaaa taattttgat
      601 gacgcccaga cagcccagcc cgccatcgtc aacgggcatt ggctgcggtt gcggctccgg
      661 ctcgacggtt tcctgaccgg taaaattcgc cgcctgaatt aataatcgac gaataattaa
      721 tgcttccagt ggacaacgat cttctgcatc aaggcggctc acaatcggca ttaatgattc
      781 ttctgtcggt tggctggcct gcactaattg tttggcaact aaatataatg gccataaggg
      841 atgttctgta tcacgcgtga gtgagatccg cggcttacgt cctttgtgca aaaccatctc
      901 gaccagtgaa cgccccagcg cagagagttt ttcatcgctg agtatttcgt ataaatgaaa
      961 acgcgtacgg gcgctgtcgc cggggacata aagtagcggt tttccctgat cgttaaccag
     1021 atcacgcgct tcaatcatca tcaccactaa tgggtaataa tcgacccgat gctgacggca
     1081 ccattgcaac attaaagcat ccgcctgggt ggagggatac tcgcgccagt agtaacaaag
     1141 ttctgccagc aggctttcgc cttctccaca atagacgcgt tgtgcgtatt cataataacg
     1201 cgcatcttca ttatcaggtt cagcttcccg ccacgaccgg gcgtaatcca ggagttcggg
     1261 gattcccagg cggcttgcgc tataccagcg gagtaaattt ctgtgcagtt ttgcatcatc
     1321 ggggataatc actgcgcgat gttgcgctaa atacaatgtc agccattctg gatgcgatga
     1381 ccaaatccga gcgaacattt ggtaaaaatc gatggtgtca ttttgcacgg caacgggcag
     1441 atgcagaatg gtctggaagt taaaaacatt ccccttcgca atggcatata aaaaggattc
     1501 cagttcttct tcgtctattt ccccgacgtt ttcctcctgc aaccaccgca atctttctgc
     1561 taataacacc acgcactcga aggataaatg ggcagtgttc atggcgattg tgcacagcga
     1621 ccagcgtaat tcatcaatct cttccatcga gcaataattt aattgctgaa tgaatcgctg
     1681 ccaggcggag ggcagaaaac gttcgctatc agaggcaagt aacgcgcgaa aggcgagcag
     1741 aatttcatgt tctgctacct catattcttc tggttgccaa acagatttag caggcgactg
     1801 cgcaatccgt agcgcctcct catacgcctg acgaagttgt ttaaaaccct gcggatcggt
     1861 ttctggatga aaggacggta acagcgccag ataagcgcgg cggataatat cgacgtcagt
     1921 cgtttcctct atatcgagga tcttccagca atttttcata agtcgctcat atccttaagc
     1981 gtttccgggc atgtggggta gtcagccagt ggttgccagg acaaggtagg gtaatacgca
     2041 tcatcactgg caaataattg ataaaacata tcattgccgg aatcgtcgta ttctttacgc
     2101 gctaagaatt cgcgtaataa aggcagatga tcatcttcgg gagactgcca atattgccga
     2161 ccaaaggtat aaccggcaaa gtattgccgc cagttactat aataacgtag agcccgtaga
     2221 tgaatacgcg acaggatcca tagactttct tcttcactca accagttatt aagtacgccc
     2281 attcgactta aaaaacccat ccggacataa tcccatgctt taattcctcc ctcaccacag
     2341 cattcggctg tgctggcgac aaattgcgca taagcctgac cttgctcatt taattcagca
     2401 agatagtcac gccattcaca tggagaataa cgaaaccagc ggtgataaaa ccctgccaac
     2461 cgagcggcat gaccgttctc gatcattcgg tgaattgtgc gcagcaggtc atcacggtca
     2521 tcaattcccc atgaggaaga aaggtcaaaa aagcatgtac ctaaatacat tttgggggat
     2581 gaatgcgaag gtaaacagca ttcattgcgt atttccatcg gagtggaaag tgcaaacaat
     2641 attttttgcg accccatgtc catataattt atccttaaac attcatttaa tgaatgcaaa
     2701 ctacatttcg agactcagtt atttatactt ttctgacgca acataatacg cataaaataa
     2761 ggcttacgca aaaatggaac atataaaaat ataatatatt ttatctacaa ccataaggaa
     2821 atgataataa tttttacgtc aagttgctgc gataatttat caatagatga gattatcgaa
     2881 cgtgctgaaa aaggcgattg cgaggctcaa tatattgttg ggttttatta taatcgcgat
     2941 agcgcaattg actctccgga cgacgaaaag gccttttact ggctgaagct ggccgctgag
     3001 caaggtcatt gtgaagcaca gtattcctta gggcaaaagt ataccgagga taaaagccgt
     3061 cataaagata atgagcaagc catcttctgg ctgaaaaaag ctgccctaca aggccatact
     3121 ttcgcttcca acgcccttgg ctggatactg gatcgtggag aagaccccaa ctataaagaa
     3181 gcggttacct ggtatcagat agccgcggag agcggaatgt cttatgcgca aaataatctt
     3241 ggatggatgt acagaaatgg caacggagtc gcacaagact atacgctggc atttttctgg
     3301 tacaaacaag ctgcattaca aggccatagt tacgcacaag acaatctggc cgatctttat
     3361 aaagacggag aaggcgttgc tcaaaacaag acactcgccg cattctggta tttgaaaagc
     3421 gcacagcagg gtaatcggca cgcccagttt caaattgcat gggattataa cgctggcgaa
     3481 ggggtggacc aggactataa gcaagcgatg tactggtatc tgaaggctgc cgctcaggaa
     3541 agcgtcggcg cttacgtcaa catcggttat atgtataaac acggacaagg cgttgagaaa
     3601 gattatcagg ctgcctttga atggtttacg aaagccgctg aatgcaatga cgccactgcc
     3661 tggtataacc tagccattat gtatcactac ggagaaggaa gacctgtcga tctccgacag
     3721 gctctcgacc tgtatcgtaa agttcagtca tccggaacca gagatgtcag tcaggaaatt
     3781 cgtgagactg aagctttact gtagaaataa aaacaagggc cggggaattt cattctgccc
     3841 atattctctt tattgctatg cgaaatcaga ctattacggc tcaaacgggc gtctctggaa
     3901 ctggtcataa ccacatttcg ggcatagcgt cagcacttcc ggtgtgtaga tcgggagatg
     3961 gaagtgacat ttctcgcaga ccagatttcc cagcccgacc acttctccgc tgtgataaac
     4021 cccatgatga ttgaggtcct ggaaaacttc gcgccattca agctgcgttt tatcggtgat
     4081 gtctgccagc tcctgccaca agctttcttt aatcacccgc ataaagacgc tgtcagattc
     4141 ttctttcagg ctctcttcat agctcatggc gaactcttcc aggtcacgtc tgacagctcg
     4201 cgtcagctca tcgagctcgg ttcgcgttaa ctcccctgtt tttattacgc gctcgcgcgc
     4261 ctgttccacc agtgcgtcga tatcacgttc gccattgcgc aggcgttcgt tcagtgacgc
     4321 gaccagttca cggtaatatt gagcaacctt gttcatcgtt tcatctcccg ggtcgttaac
     4381 tgactataat aatagacgtt ctttgttcct tgctttgcta atacgcccac aaactctgta
     4441 aattaatgta cgaataatcc gggcatcatt tcaacgtcga gtgcgcgaaa ggctgttttg
     4501 acgcggacgt ttgggctatg ctatgcggat ctgaaaaacc acctcaacgc tacatttgta
     4561 gccgtattga aaacaggacc actggctgcc atgcaagagc aataccgccc ggaagagata
     4621 gaatccaaag tacagcttca ttgggatgag aagcgcacat ttgaagtaac cgaagacgag
     4681 agcaaagaga agtattactg cctgtctatg cttccctatc cttctggtcg actacacatg
     4741 ggccacgtac gtaactacac catcggtgac gtgatcgccc gttaccagcg catgctgggc
     4801 aaaaacgttc tgcagccgat cggctgggac gcgtttggtc tgcctgcgga aggcgcggcg
     4861 gtgaaaaaca acaccgctcc ggcaccgtgg acgtacgaca acatcgcgta tatgaaaaac
     4921 cagctcaaaa tgctgggctt tggttatgac tggagccgcg agctggcaac ctgtacgccg
     4981 gaatactacc gttgggaaca gaaattcttc accgagctgt ataaaaaagg cctggtatat
     5041 aaaaagactt ctgcggtcaa ctggtgcccg aacgaccaga ccgtactggc gaacgaacaa
     5101 gttatcgacg gctgctgctg gcgctgcgat accaaagttg aacgtaaaga gatcccgcag
     5161 tggtttatca aaatcactgc ttacgctgac gagctgctca acgatctgga taaactggat
     5221 cactggccag acaccgttaa aaccatgcag cgtaactgga tcggtcgttc cgaaggcgtg
     5281 gagatcacct tcaacgttaa cgactatgac aacacgctga ccgtttacac tacccgcccg
     5341 gacaccttta tgggttgtac ctacctggcg gtagctgcgg gtcatccgct ggcgcagaaa
     5401 gcggcggaaa ataatcctga actggcggcc tttattgacg aatgccgtaa caccaaagtt
     5461 gccgaagctg aaatggcgac gctggagaaa aaaggcgtcg atactggctt taaagctgtt
     5521 cacccattaa cgggcgaaga aattcccgtt tgggcagcaa acttcgtatt gatggagtac
     5581 ggcacgggcg cagttatggc ggttccgggt cacgaccagc gcgactacga gtttgcctct
     5641 aaatacggcc tgaacatcaa accggttatc ctggcggctg acggctctga gccagatctt
     5701 tctcagcaag ccctgactga aaaaggcgtg ctgttcaact ctggcgagtt caacggtctt
     5761 gaccatgaag cggccttcaa cgccatcgcc gataaactga ctgcgatggg cgttggcgag
     5821 cgtaaagtga actaccgcct gcgcgactgg ggtgtttctc gtcagcgtta ctggggcgcg
     5881 ccgattccga tggtgacgct ggaagacggt accgtaatgc caaccccgga cgaccagctg
     5941 ccggtgatcc tgccggaaga tgtggtgatg gacggcatta ccagcccgat taaagcagat
     6001 ccggagtggg caaaaactac cgttaacggt atgccagcgc tgcgtgaaac cgacactttc
     6061 gacaccttta tggagtcctc ctggtactat gcgcgctaca cttgcccaga gtacaaagaa
     6121 ggtatgctgg attccgaagc ggctaactac tggctgccgg tggatatcta cattggtggt
     6181 atcgaacacg ccattatgca cctgctctac ttccgcttct tccacaaact gatgcgtgat
     6241 gcaggcatgg tgaactctga cgaaccagcg aaacagttgc tgtgtcaggg tatggtgctg
     6301 gcagatgcct tctactatgt tggcgaaaac ggcgaacgta actgggtttc cccggttgat
     6361 gctatcgttg aacgtgacga gaaaggccgt atcgtgaaag cgaaagatgc ggcaggccat
     6421 gaactggttt ataccggcat gagcaaaatg tccaagtcga agaacaacgg tatcgacccg
     6481 caggtgatgg ttgaacgtta cggcgcggac accgttcgtc tgtttatgat gtttgcttct
     6541 ccggctgata tgactctcga atggcaggaa tccggtgtgg aaggggctaa ccgcttcctg
     6601 aaacgtgtct ggaaactggt gtacgagcac acagcaaaag gtgatgttgc ggcactgaac
     6661 gttgatgcgc tgactgaaga tcagaaagcg ctgcgtcgcg atgtgcataa aactatcgct
     6721 aaagtgaccg atgatatcgg ccgtcgtcag accttcaaca ccgcaattgc ggcgattatg
     6781 gagctgatga acaaactggc gaaagcaccg accgatggcg agcaggatcg cgctctgatg
     6841 caggaagcac tgctggccgt tgtccgtatg ctcaacccgt tcaccccgca catctgcttc
     6901 acgctgtggc aggaactcaa aggcgaaggc gatatcgaca acgcgccgtg gccggttgct
     6961 gacgaaaaag cgatggtgga agactccacg ctggtcgtgg tgcaggttaa cggtaaagtc
     7021 cgtgccaaaa tcaccgttcc ggtggacgca acggaagaac aggttcgcga acgtgctggc
     7081 caggaacatc tggtagcaaa atatcttgat ggcgttactg tacgtaaagt gatttacgta
     7141 ccaggtaaac tcctcaatct ggtcgttggc taagcgcggg aggaagcgtg cgatatctgg
     7201 caacattgtt gttatctctg gcggtgttaa tcaccgccgg gtgtggctgg catctgcgtg
     7261 acaccacgca ggttccttcc actatgaagg tcatgatcct ggactcaggc gatccgaacg
     7321 ggccattaag ccgtgcggtg cgtaaccagt tacgtctgaa tggtgtcgag ttgcttgata
     7381 aagaaaccac gcgtaaggac gttccatccc tgcgtttggg taaagtgagc atcgcgaaag
     7441 ataccgcatc ggtattccgt aacggtcaaa cagcagagta tcagatgatc atgacggtga
     7501 atgcgaccgt gttgatcccc ggccgtgata tctacccaat tagcgccaaa gtcttccgtt
     7561 cattcttcga taacccgcaa atggcgttag cgaaagataa cgaacaagac atgatcgtaa
     7621 aagagatgta cgaccgtgct gccgaacagc tgattcgtaa gctgccaagc atccgtgctg
     7681 cggatattcg ttccgacgaa gaacagacgt cgaccacaac ggatactccg gcaacgcctg
     7741 cgcgcgtctc caccatgctg ggtaactgat gattcggttg tacccggaac aactccgcgc
     7801 gcagctcaat gaagggctgc gcgcggcgta tcttttactt ggtaacgatc ctctgttatt
     7861 gcaggaaagc caggacgctg ttcgccaggt agctgcggca caaggattcg aagaacacca
     7921 cactttttcc attgatccca acactgactg gaatgcgatc ttttcgttat gccaggctat
     7981 gagtctgttt gccagtcgac aaacgctatt gctgttgtta ccagaaaacg gaccgaatgc
     8041 ggcgatcaat gaacaacttc tcacactcac cggacttctg catgacgacc tgctgttgat
     8101 cgtccgcggt aataaattaa gcaaagcgca agaaaatgcc gcctggttta ctgctcttgc
     8161 gaatcgcagc gtgcaggtga cctgtcagac accggagcag gctcagcttc cccgctgggt
     8221 tgctgtgcgc gcaaaacagc tcaacttaga actggatgac gcggcgaatc aggtgctctg
     8281 ctactgttat gaaggtaacc tgctggcgct ggctcaggca ctggagcgtt tatcgctgct
     8341 ctggccagac ggcaaactga cattaccgcg cgttgaacag gcggtgaatg atgccgcgca
     8401 tttcacccct tttcattggg ttgatgcttt gttgatggga aaaagtaagc gcgcgttgca
     8461 tattcttcag caactgcgtc tggaaggcag cgagccggtt attttgttgc gcacattaca
     8521 acgtgaactg ttgttactgg ttaacctgaa acgccagtct gcccatacgc cactgcgtgc
     8581 gttgtttgat aagcatcggg tatggcagaa ccgccggggc atgatgggcg aggcgttaaa
     8641 tcgcttaagt cagccgcagt tacgtcaggc cgtgcaactc ctgacacgaa cggaactcac
     8701 cctcaaacaa gattacggtc agtcagtgtg ggcagagctg gaagggttat ctcttctgtt
     8761 gtgccataaa cccctggcgg acgtatttat cgacggttga tatgaaatct ttacaggctc
     8821 tgtttggcgg cacctttgat ccggtgcact atggtcatct aaaacccgtg gaaacgctgg
     8881 cgaatttgat tggtctgacg cgggtcacaa tcatccctaa taatgttcct ccgcatcgtc
     8941 cccagccgga agcgaacagc gtgcagcgta aacacatgct tgaactggcg attgccgaca
     9001 agccattatt tactcttgat gaacgcgagc taaagcgcaa tgccccctct tacactgcac
     9061 aaacactgaa agagtggcgg caggaacaag gaccggacgt gccgctggcg tttattattg
     9121 gtcaggattc attgctgacc tttccgacct ggtacgaata cgaaacgata ctcgacaatg
     9181 cacatttgat cgtctgtcgg cgtccaggtt acccacttga aatggcgcaa ccgcaatacc
     9241 agcaatggct ggaagatcat ttgacacata acccggaaga tcttcacctt cagcctgccg
     9301 gtaaaattta tctggctgaa acgccgtggt ttaacatctc ggcgaccatc atccgcgaac
     9361 gtttgcaaaa cggtgaatcg tgtgaggatt tattgccgga accggtattg acttacatta
     9421 accaacaagg cttgtatcgc tgatacctgt cgttacggag ggcatgatgc gactgtggtt
     9481 aattcgtcat ggcgaaacac aagcgaatgt cgatggtctg tacagcggtc atgcgcccac
     9541 acccctgacc gcacgcggta ttgagcaagc gcaaaatctg catacactgc tagatgatgt
     9601 ttcctttgac ctggttttat gcagtgaact ggaacgggca cagcataccg cgcgactggt
     9661 tctcagtgac cgccagctcc ccgttcatat catccctgaa ctcaacgaaa tgttttttgg
     9721 cgactgggag atgcgacatc atcgcgacct catgcaagaa gatgccgaaa actatagcgc
     9781 gtggtgcaat gactggcagc atgctatccc cacgaacggt gaaggatttc aggcattttc
     9841 gcaacgtgtg gaacgcttta tcgcaaggct tagtgaatat cagcactatc agaatatttt
     9901 aatcgtcagc catcagggcg tactgagtct gttaattgct cgtttaattg gcatgcctgc
     9961 cgagtctatg tggcattttc gcgttgacca ggggtgttgg agcgccattg atatcaacca
    10021 aaaattcgcg acgctacgcg tcctcaatag ccgtgccatc ggggtcgaga atgcatgact
    10081 tttctgtttt tttacgggta agccgcaacg accattgaca gccccgggca ggctgatatt
    10141 ctccgcagcc agactttttc cgccagacac gactttgtag aaattgtttt acaaaaatgg
    10201 cgatgcaatc tgcggcgcgg ggtgggatga tagcccactt tcgaaagccg attcggcgac
    10261 aattgtcccg aaatcgcctc tggttcaggt atactgacag accattttta tctatttgat
    10321 tcacccaggg ggaaaacttg cagggtaaag cactccagga ttttgttatc gacaaaattg
    10381 atgacctcaa aggtcaggac atcatcgcct tagacgttca aggcaaatcc agcatcaccg
    10441 actgcatgat catctgtacg ggtacgtcca gccgtcatgt tatgtccatt gctgaccacg
    10501 ttgtgcagga gtctcgcgca gcgggcctgt taccgctcgg cgtagaaggt gaaaacagcg
    10561 ccgactggat tgtcgtggat ttgggcgatg tgattgtcca tgtcatgcag gaagagagcc
    10621 gtcgcctgta tgaactggaa aaactctgga gttaatgcgt gaagctgcaa cttgtcgccg
    10681 tgggaacgaa aatgccggac tgggtacaaa ccggttttac cgagtacctg cgtcgttttc
    10741 cgaaagatat gcccttcgag ctgattgaaa ttccggccgg caaacgcggc aagaatgcgg
    10801 acatcaagcg catactcgac aaagagggtg agcagatgtt ggcggccgca ggcaaaaacc
    10861 gcattgtcat cctcgatatt ccaggcaagc cctgggatac gccgcagtta gccgctgagc
    10921 tggaacgctg gaagctggat ggtcgcgacg taagtctact gattggcggg cctgaagggt
    10981 tgtcgcctgc ctgtaaagcg gcggctgagc agagctggtc gctgtcggcg cttaccctcc
    11041 cccatccgct ggttcgcgtg ctggtcgcag agagtctgta ccgggcgtgg agcatcacca
    11101 ccaaccatcc ttatcaccgt gagtgataag ggagctttga gtagaaaacg cagcggatga
    11161 aactacagaa ctcttttcgc gactatacgg cagagtccgc gctgtttgtg cgccgggcgc
    11221 tggtcgcctt tttggggatt ttgctgctga ccggcgtgct tatcgccaac ctgtataatc
    11281 tgcaaattgt tcgctttacc gactaccaga cccgctctaa tgaaaaccgc attaagctgg
    11341 tgcctatcgc gcccagccgc ggcattatct acgaccgtaa cggtatccct ctggccctca
    11401 accgcactat ctaccagata gaaatgatgc cggagaaagt cgataacgtg cagcaaacgc
    11461 tggacgcttt gcgcagcgtg gtagatctga ccgatgacga tattgctgca ttccgaaaag
    11521 agcgcgcacg ttcacaccgt ttcacctcta ttccggtgaa aactaacctg accgaagtac
    11581 aagtagctcg ctttgccgtc aatcagtacc gttttccggg tgtcgaagtt aaaggctata
    11641 aacgtcgtta ctatccttac ggttcggcgt tgacccacgt catcggatat gtgtcgaaaa
    11701 tcaacgataa agacgtcgaa cgcctgaata atgacggcaa actggccaac tatgcggcaa
    11761 cgcatgatat tggtaagctg ggcattgagc gttactatga agacgtgctg cacggtcaga
    11821 ccggttatga agaggttgaa gttaacaacc gtgggcgtgt tatccgccag ttaaaagaag
    11881 taccaccgca agccggacac gatatttacc tgacgctgga tctcaaactc cagcaatata
    11941 ttgaaacgct gctggcgggt agccgcgcag ctgtggttgt taccgatccg cgtacaggtg
    12001 gggtgctggc gctggtttcc acgcctagtt atgacccaaa cctgtttgtt gacggtatct
    12061 ccagcaaaga ttattccgcc ttgctgaacg acccgaatac accgctggtg aaccgcgcca
    12121 cacagggggt ttatcctccc gcgtctacag ttaaacccta tgtggcggtt tcggcattaa
    12181 gcgccggggt gatcacgcgc aatacgacgc tgtttgaccc aggctggtgg caactaccag
    12241 ggtcggaaaa acgttatcgt gactggaaaa aatggggcca cgggcgtctg aatgtcacaa
    12301 gatcgctgga agaatctgcg gataccttct tctatcaggt ggcctacgat atggggatcg
    12361 atcgcctctc cgaatggatg ggtaaattcg gttatggtca ttacaccggt atcgacctgg
    12421 cggaagaacg ttccggcaac atgcctaccc gcgaatggaa acagaaacgc tttaaaaaac
    12481 cgtggtatca gggtgacacc attccggttg gtatcggcca gggttactgg acagcgaccc
    12541 caatccagat gagtaaggca ctgatgatcc tgattaatga cggtatcgtg aaggttccgc
    12601 atttgctgat gagcaccgcc gaagacggca aacaggtgcc atggctacaa ccgcatgaac
    12661 cgcccgtcgg cgatattcat tccggttact gggagctggc gaaagacggt atgtacggtg
    12721 ttgctaaccg ccctaatggt acggcgcata aatactttgc cagcgcaccg tacaaaattg
    12781 cggcgaaatc tggtaccgct caggtcttcg gtctgaaagc gaacgaaacc tataatgcgc
    12841 acaaaattgc cgagcgttta cgtgaccaca aactgatgac cgcctttgcg ccatacaaca
    12901 atccgcaagt ggctgtcgcc atgattctgg agaacggcgg cgcgggtccg gcggttggta
    12961 cgctgatgcg ccagatcctc gaccacatta tgctgggtga taacaacacc gatctgcctg
    13021 cggaaaatcc agcggttgcc gcagcggagg accattaatc atgacggata atccgaataa
    13081 aaaaacattc tgggataaag tccatctcga tcccacaatg ctgctgattt tactggcatt
    13141 gctggtttac agcgccctgg ttatctggag cgccagcggt caggatattg gcatgatgga
    13201 gcgtaaaatc ggccaaatcg cgatgggtct ggtcatcatg gtggtgatgg cgcaaattcc
    13261 tccacgcgtt tatgaaggct gggcccccta tctctatatc atctgtatta ttttgctggt
    13321 ggcggtagat gctttcggag ccatctctaa aggcgctcaa cgctggctgg acctcggtat
    13381 tgttcgtttt cagccgtcgg aaattgccaa aatagccgta ccactgatgg ttgcgcgctt
    13441 tatcaaccgc gacgtttgcc cgccatcgtt gaagaacact ggcatcgcgc tggtgctgat
    13501 atttatgccc acgctgctgg tggctgcaca gcctgacctg ggaacatcaa tcctcgttgc
    13561 gctttccggt ctgtttgtac tgttcctctc tggccttagc tggcgtctga ttggcgtcgc
    13621 agtagtgctg gtagcggcgt tcattccgat tctgtggttc ttcctgatgc atgattacca
    13681 gcgccagcgc gtaatgatgc tcctggaccc ggaatcagac ccactcggcg cgggctatca
    13741 cattattcag tctaaaattg ctattggctc cggcggatta cgcggcaaag gctggctgca
    13801 cggcactcag tcacagcttg aatttctccc cgaacgccat actgacttta tcttcgcggt
    13861 actggcggaa gagctgggat tagtgggcat tctgattctg ctcgctctct acattctgct
    13921 gatcatgcgc gggctgtgga tagccgccag agcgcaaacc acctttggtc gcgtcatggc
    13981 tggtggctta atgctgatat tattcgttta tgtcttcgta aatattggta tggtaagcgg
    14041 tattctgccg gttgtagggg ttccgctccc actggtcagt tatggaggat cggcgctaat
    14101 tgtgctgatg gctgggttcg ggattgtaat gtcaatccac acccacagga aaatgttgtc
    14161 gaaaagcgtg taagaggtgc gcaatgcgta agcagtggct cgggatctgc atcgcggcag
    14221 gaatgctcgc ggcatgtaca agcgatgatg gtcagcaaca gacggtaagt gtaccgcagc
    14281 ctgcggtatg taacggcccc atagttgaaa ttagcggggc ggacccgcgt ttcgaaccac
    14341 tgaacgcgac ggcaaatcag gattaccagc gcgacggtaa aagctacaaa atcgtgcagg
    14401 atccgtctcg atttagccag gcgggactgg cggcaatcta tgatgccgaa ccaggcagta
    14461 acctgacggc ctctggcgaa gctttcgatc cgacaaagct gacggcggcc catccaacgc
    14521 ttccgatccc cagctacgcc agaatcacta acctggctaa cgggcgaatg atcgtggtgc
    14581 gcattaatga tcgcggccct tacggcaacg accgcgttat ttcgctttct cgcgcggcag
    14641 ctgaccgtct taacacgtca aacaacacca aagttcgtat cgatccgatt attgtcgccc
    14701 aggatggttc gctttctggt cctggtatgg cttgtaccac agtcgccaaa cagacttatg
    14761 ccctgcctgc acctcccgat ttaagcggtg gcgcgggaac aagttcagtg tctggcccgc
    14821 agggtgacat tcttccggtc agtaattcga cgctaaaaag cgaagatccg accggcgcgc
    14881 cggtaaccag tagcggtttc ctcggcgctc aaacgacctt agcgcctggt gtactggaag
    14941 gcagcgaacc gacgcctgcg ccacagcccg ttgttacagc ttcgtcgaca acgcctgcaa
    15001 cctcgcctgc aatggtgaca ccgcaagccg cctcgcaaag cgccagcggc aactttatgg
    15061 tgcaagtcgg ggccgtaagc gatcaggctc gtgcgcaaca gtaccaacag caactgggac
    15121 agaagttcgg cgtccccggt cgcgtaactc aaaatggcgc ggtctggcgg atccagcttg
    15181 gcccattcgc cagcaaagcc gaagccagta ccttgcagca acgtttgcaa accgaagccc
    15241 aattacagtc atttattact accgcgcagt agcgtaaagc aggcatctga tgtgtcaatt
    15301 tctgtaaatg acgttaacaa agtcacgcgg aaagtcagat gcctgccggt agtgcatttg
    15361 ctatagtagg gcactttttt aattccatca cggatgtcgt agttcagacc atgaatacca
    15421 ttttttccgc tcgtatcatg aagcgcctgg cgctcaccac ggctctttgc acagccttta
    15481 tctctgctgc acatgccgat gacctgaata tcaaaactat gatcccgggt gtaccgcaga
    15541 tcgatgcgga gtcctacatc ctgattgact ataactccgg caaagtgctc gccgaacaga
    15601 acgcagatgt ccgccgcgat cctgccagcc tgaccaaaat gatgaccagt tacgttatcg
    15661 gccaggcaat gaaagccggt aaatttaaag aaactgattt agtcactatc ggcaacgacg
    15721 catgggccac cggtaacccg gtgtttaaag gttcttcgct gatgttcctc aaaccgggca
    15781 tgcaggttcc ggtttctcag ctgatccgcg gtattaacct gcaatcgggt aacgatgctt
    15841 gtgtcgccat ggccgatttt gccgctggta gccaggacgc ttttgttggc ttgatgaaca
    15901 gctacgttaa cgcactgggc ctgaaaaata cccacttcca gacggtacat ggtctggatg
    15961 ctgatggtca gtacagctcc gcgcgagata tggcgctgat cggccaggca ttgatccgtg
    16021 acgtaccgaa tgaatactcg atctataaag aaaaagaatt tacgtttaac ggtattcgcc
    16081 agctgaaccg taacggcctg ttatgggata acagcctgaa tgtcgacggc atcaaaaccg
    16141 gacacactga caaagcaggt tacaaccttg ttgcttctgc gactgaaggc cagatgcgct
    16201 tgatttctgc ggtgatgggt ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac
    16261 tgctaacctg gggcttccgt ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt
    16321 tcgcctcaga accggtttgg tttagtgatt ctgatcgcgc ttcgttaggg gttgataaag
    16381 acgtgtacct gaccattccg cgtggtcgca tgaaagatct gaaagccagc tatgtgctga
    16441 acagcagtga attgcatgcg ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc
    16501 agcttgatgg caaaacgatc gagcaacgcc cgctggttgt gttgcaagaa atcccggaag
    16561 gtaacttctt cggcaaaatc attgattaca ttaaattaat gttccatcac tggtttggtt
    16621 aaaaattgaa cacttgaaag tgtaatttcc gtccccatat actaagcatc agtaaaaaaa
    16681 ctcccacctt ctggcgggag ttgctattta attacgttac gccggagctg acatgaaaac
    16741 caaacttaac gaactgcttg aattccctac tccttttact tacaaagtta tggggcaggc
    16801 gttacctgag ctggttgatc aggtggttga agtggtacag cgccatgcgc caggtgacta
    16861 caccccaacg gtaaaaccaa gcagcaaagg caactaccac tcggtatcta tcactatcaa
    16921 cgccactcat atcgagcagg ttgaaacact gtatgaagaa ctgggcaaaa tcgatattgt
    16981 ccgcatggtt ctgtaactcg cttctccgtt acccggctcc tggtcgggta actctcctct
    17041 cctcgctgtg atatactttt cccccacttt tactcattct ccacggagat gccgttttgt
    17101 atcaggataa aattcttgtc cgccagctcg gtcttcagcc ttacgagcca atctcccggg
    17161 ctatgcatga attcaccgat acccgcgata atagtaccct tgatgaaatc tggctggtcg
    17221 agcactatcc ggtattcacc caaggtcagg caggaaaagc ggagcacatt ttaatgccgg
    17281 gtgatattcc ggtgatccag agcgatcgcg gtgggcaggt gacttatcac gggccgggac
    17341 aacaggtgat gtatgtgttg cttaacctga aacgccgtaa actcggtgtg cgtgaactgg
    17401 tgaccttgct tgagcaaaca gtggtgaata ccctggctga actgggtata gaagcgcatc
    17461 ctcgggctga cgcgcctggt gtctatgtcg gggaaaagaa aatttgctca ctgggtttac
    17521 gaattcgacg cggttgttca ttccacggtc tggcattaaa cgtcaatatg gatctttcac
    17581 catttttacg tattaatcct tgtgggtatg ccggaatgga aatggctaaa atatcacaat
    17641 ggaaacccga agcgacgact aataatattg ctccacgttt actggaaaat attttagcgc
    17701 tactaaacaa tccggacttc gaatatatta ccgcttaatt ccatacatca atggcccaat
    17761 ttacactggg tcattactca taatacctgc taatgcttta catctccttc gcgtttgtat
    17821 attttcttta tcacttgcag aaatgcggtg ccacgccttg ctccgtaact caccccattg
    17881 attaacctat tgaaatagtt gattatggtg agcaaaagcg ttccgttcca attagacata
    17941 agattattgt aattttttaa atattatttt tccatgaata aaattggagt aagtgcgtgg
    18001 atagtaataa tcaaattgaa ccctgtttaa gcagaaagtc gtcagaaggt aaaccacaaa
    18061 tatttacaac cttacgaaat attgatctta accttctgac tatttttgaa gctgtatatg
    18121 tacataaagg gatcgttaat gcagcgaaag tgcttaatct gaccccctcg gcaatcagtc
    18181 agtctattca gaaactgcgc gttatattcc ctgacccatt gtttattcgc aaaggccaga
    18241 gtgtcactcc taccgcattt gcgatgcatc tacatgagta tatcagtcag ggccttgagt
    18301 ccattcttgg cgcgctggat atcgaaggaa gctatgataa gcaacgaacg ataactattg
    18361 cgaccactcc ctcggtcgga gccctggtcc ttcctgtcat ctatcgggcg attaaaactc
    18421 actatccgca gcttttactg cgcaacccgc ccatcagcga cgcggaaaac caactcagtc
    18481 agtttcaaac cgatctcatc atcgataaca tgttttgcac caatcgtacg gtgcaacatc
    18541 atgttctgtt caccgacaat atggtgttaa tttgccgcaa gggaaatcca ctactctctt
    18601 tagaagatga cagagagact atcgacaacg ctgcgcatgt acttctgtta ccggaagggc
    18661 aaaatttcag cggtctgcgg cagagagttc aagagatgtt tccggaccgg caaatcaatt
    18721 tcaccagcta caacattttg acaatcgctg cactggttgc caacagtgac atgttagcga
    18781 ttattcccag ccgtttttat aacctgttta gtcgttgccg gccgctggaa aaactgcctt
    18841 ttccgtcctt aaatgaggag caaatagatt tctctatcca ctacaacaaa ttcagcctgc
    18901 gtgatcccat cctgcacgga gtaatagatg ttatccgtaa tgcattttga aaaagtattg
    18961 ttacagccag atcacagtgc agaacgataa aatggcacaa tccaacaaca attttacatt
    19021 ttcgcgaccg ctttggctgc tttcaggtcc gtttcaatga tatactgcca gtcgttaatt
    19081 caaaaatagt tgataattac aacaatctat tgaattgaaa cgctttcctt cgtaattcgc
    19141 aactggaaca cgcacgctat gagtaaaccc attgtgatgg aacgcggtgt taaataccgc
    19201 gatgccgata agatggccct tatcccggtt aaaaacgtgg caacagagcg cgaagccctg
    19261 ctgcgcaagc cggaatggat gaaaatcaag cttccagcgg actctacacg tatccagggc
    19321 atcaaagctg caatgcgcaa aaatggcctg cattctgtct gcgaggaagc ctcctgccct
    19381 aacctggcgg aatgcttcaa ccacggcaca gcaacgttta tgatcctcgg cgctatttgt
    19441 acccgccgtt gtccgttctg tgatgttgcc cacggtcgcc cggtagctcc tgatgccaat
    19501 gaaccagtga aactggcgca gaccattgcc gatatggcgc tgcgttatgt ggttatcacc
    19561 tccgttgacc gtgatgacct gcgcgatggc ggtgcccaac actttgcgga ttgtattact
    19621 gccattcggg aaaaaagccc gcaaatcaaa attgaaactc tggtgccgga tttccgcggt
    19681 cgaatggatc gtgctctgga tattctgact gcaacgcccc cagatgtgtt caaccataac
    19741 ctggaaaacg taccgcgtat ttaccggcag gtacggcctg gcgcagatta caactggtcg
    19801 ctgaagctgc tggaacgctt taaagaagcg catccggaaa tcccgaccaa gtctggtctg
    19861 atggtgggtc tgggtgaaac caatgaagaa attattgagg taatgcgcga cctgcgccgt
    19921 catggtgtga cgatgttaac gctggggcaa tatttgcagc caagccgcca tcacctgccg
    19981 gttcaacgtt acgttagccc ggatgagttc gacgaaatga aagccgaagc gctggcgatg
    20041 ggctttaccc atgctgcatg cggtccgttt gtccgctctt cttaccacgc cgatttgcag
    20101 gcgaaaggga tggaagttaa gtaatcctgt aataattact tacatctgtc tgataaaaaa
    20161 cccgcttttt gaagcgggtt ttttgtatcg aacaagatat tgagggagcg tcctgctcgc
    20221 cacgtcactc tttatgagag agcttttcag cctgaagatc aacgtctgcg ccttttttcg
    20281 ccgcagcatc gtcatcattc atcgccttct tgaacccttt aatggccgct ccaaggtctc
    20341 cgcccagcgt acgtaacttc ttagtcccaa acagcagaac gaccagcgcc gcaactacca
    20401 gcagtttggt aatactaatc tcacccatag ataccttctt gacatataac cggcaacaga
    20461 acgcgcctta aaaaccagag aatattaacg gtcattcgac gcgcgcacac aatagcaatc
    20521 tgtaacaagg tgaatcaagc gccgagtaaa aaaacatcat aataattgcg gcggcgcaaa
    20581 gcgacggttg tttaagatgg gcagttgcgc gcgcacctga cgcacacgtt cgggcgtcac
    20641 ttccgccata atgagtgcag gcatttctga cgctgccgca atggtgacac caaagggatc
    20701 tataatccgg ctttgcccga tatttttgtt cccgcactcc cccgccgcca ccatataaca
    20761 ggtggtatcc agcgcacgag cggcaagcaa cgttgaccag tgatgctctt tgagcggccc
    20821 acgaacccag gcggcaggaa gtaccaggat ttcagctccc tgtaaagcct gtgccagcgc
    20881 cagctctgga aagcgtaagt cataacaggt catcagaccg accttcatcc cttccacctc
    20941 cagtaacgga gctatttcat taccagcatc aacatggcgt gattcctgaa tggcaaatgc
    21001 atcatagaga tgcagtttgg cataacgggc gacgatgttt cctgcctgaa gtgccaccag
    21061 catattccat gcgcgccccg gcgttgaagg aagatgaatc gtcagaattg tcgtcatcat
    21121 gttacgttta ctttctcgcc gtaaacgtcc gaggaattcg ccttccagca gctgtgctga
    21181 tttaaccgat agatctgcat catgatcatc gcgcgccagc aatgcttccg gcaggacaaa
    21241 cagcgatacg tcgttttccg ccgcctgcgc catcaacgag gcacaaatct cagcgttctt
    21301 ttcccacaca gatgtaacag caaactgtcc tgcagcaacc agcattgcca cctcctgtca
    21361 gagtcgtaaa attcgccaac gacgattatt cggcgttaca cttatcactc atacaaatca
    21421 aatagcagga ttttgcagtg ttacaacttc ttttagcagt ttttattggc ggtggtacgg
    21481 gaagcgtagc gagatggctg ttaagtatgc gatttaaccc gctgcatcag gcgattccgt
    21541 tggggacgct ggcagcaaac ctgattgggg cattcatcat aggaatggga ttcgcctggt
    21601 tcagcaggat gacgaacatt gatccagtgt ggaaagtatt aatcaccacc ggattttgtg
    21661 gcggtctaac aaccttctca acattttcgg cagaagtggt gtttttgtta caagagggcc
    21721 gctttggctg ggcattactg aacgttttcg tcaaccttct ggggtctttt gccatgaccg
    21781 cactggcatt ctggctgttt tcggcctcaa ccgcacacta aagaccaaaa aaaacccgct
    21841 gattaagcgg gttttgaatt cttgctgacg tatcttacag agcgattacg tttgcagcag
    21901 aagggccttt ggcaccgtta gtgatttcga actctacgcg ctgaccttca gcaagagttt
    21961 taaaaccatt agtctggatt gcagagaagt gtacgaacac gtctttgctg ccgtcttccg
    22021 gagtaatgaa accgaatcct ttggactcat taaaccactt aacgttacct ttaatcttag
    22081 acatcaaaat tacctttaca tgaaaaatag acacaaatgc tgtgtcggtt atcagtacac
    22141 caattgtggt acgctttgtc cagtgcaact ttactaaaaa gtgataaaag tcgcgttatt
    22201 tttgatgttg cttattttag ttattgattt taaagaagtt actaaaactt catttgcctc
    22261 aaaactgaaa gcgcatccag gcaaagtaca cattgccatt gttgtaggta cccggaatgt
    22321 aggtcatctg aaaagtcact gggccataac ccacggaggc caatggcagt agaaccggga
    22381 gagggatgta attccagtta tcgcgtgccg ttacgccagc ggtgaatccc agacctaaat
    22441 gaaaattttc atccgccagc ggtcgccagg tactttccca tccgtatccg gcaatcggtt
    22501 cccatttgtt ccacgagtcc ttaaatgcca tggcatacag gccatgccag tttccttttt
    22561 catcccaacg cgacaggcca aaaccgccac cccacggtcg ctcgttatag cgatcggttt
    22621 tttctttgtc gtaagcgaaa cgtgcatgcc aggtgatggc aggaatatat aaatcataat
    22681 gttcaggctg ttgccaggtt tgtgcaatat tttctctaaa cgttgtcatc ctctcatctg
    22741 cgttagcaaa aactttacca acgctgatta actgaataaa aacaaaggaa aagatagcga
    22801 catatttact cacgttcatt tgtgaccata aaacatttat caaaaatcta ctactagcat
    22861 agcaaagcta caattaacat aaccttaata gacccaacat aaagaataat ctgaatagct
    22921 gctctattta tttccgcttg ttcaagttaa atatttccgc gcatcgatta aagatgatgc
    22981 gtcgttttct gcgatgggaa tagtcaaaaa agaaaaacca agtctttttt gatgacaaaa
    23041 tgcaatcaag gaaaaattat tttttttaac gagatacaac aatcatctta acgaagtata
    23101 taatatatat cgtcaaccca tgctcatatt agattatttt ttgagcgcac agccgcagct
    23161 aacaccattg caattaacaa atttgcatca atccaccatc aatttacaca cattattatg
    23221 tgataattgc caaccgctaa atatgcgttt tgttatctat gtataaaaac agcaacttca
    23281 atgtcttaat ggcagttttt cttgatttta atcagcattc atcgccaatt tattgggcat
    23341 attttttcct taagctttag gaagttttta ttatttactt tgggcctgga gacaggaaaa
    23401 attatgctga cattcattga actccttatt ggggttgtgg ttattgtggg tgtagctcgc
    23461 tacatcatta aagggtattc cgccactggt gtgttatttg tcggtggcct gttattgctg
    23521 attatcagtg ccattatggg gcacaaagtg ttaccgtcca gccaggcttc aacaggctac
    23581 agcgccacgg atatcgttga atacgttaaa atattactaa tgagccgcgg cggcgacctc
    23641 ggcatgatga ttatgatgct gtgtggcttt gccgcttaca tgacccatat cggcgcgaat
    23701 gatatggtgg tcaagctggc gtcaaaacca ttgcagtata ttaactcccc ttacctgctg
    23761 atgattgccg cctattttgt tgcttgtctg atgtcactgg ccgtctcttc cgcaaccggt
    23821 ctgggtgttt tgctgatggc aaccctgttt ccggtgatgg taaacgttgg tatcagtcgt
    23881 ggcgcagcag ctgccatttg tgcctccccg gcggcgatta ttctcgcacc gacttcaggg
    23941 gatgtggtgc tggcggcgca ggcttccgaa atgtcgctga ttgacttcgc cttcaaaacg
    24001 acgctgccta tctcaattgc tgcaattatc ggcatggcga tcgcccactt cttctggcaa
    24061 cgttatctgg ataaaaaaga gcacatctct catgaaatgt tagatgtcag tgaaatcacc
    24121 accactgccc ccgcgtttta tgccattttg ccgttcacgc cgatcatcgg tgtactgatt
    24181 tttgacggta aatggggtcc gcaattacac atcatcacta ttctggtgat ttgtatgctg
    24241 attgcctcca ttctggagtt cctccgcagc tttaataccc agaaagtttt ctctggtctg
    24301 gaagtggctt atcgcgggat ggcagatgcg tttgctaacg tggtgatgct gctggttgcc
    24361 gctggggtat tcgctcaggg gcttagcacc atcggcttta ttcaaagtct gatttctatc
    24421 gctacctcgt ttggttcggc gagtatcatc ctgatgctgg tattggtgat tctgaccatg
    24481 ctggcggcag tcacgaccgg ttcaggcaat gcgccgtttt atgcgtttgt tgagatgatc
    24541 ccgaaactgg cgcactcttc cggcattaac ccggcgtatt tgactatccc gatgctgcag
    24601 gcgtcaaacc tgggtcgtac cctatcaccc gtttctggcg tagtcgttgc ggttgccggg
    24661 atggcgaaaa tctcaccatt tgaagtcgta aaacgcacct cggtgccggt gcttgttggg
    24721 ctagtgattg ttatcgttgc tacagagctg atggtgccag gaacggcagc agcggtcaca
    24781 ggcaagtaaa tagtaatgcc ggaggcaagt ttcctccggc tttatcatgt cacccactgt
    24841 ggtatatgcg ttgtggtctg ccaactttgc cgtgaacaat ttcagcaata atcagatggc
    24901 ggctggcgca atattcaaga taacgcctgg cagtggtgcg gctgatggtc agtgcctgcg
    24961 ccaccgtttc tgccgtatgt tgcacaccag gctctttaaa cagttttcgc accgcgttta
    25021 gcgtcaaggg gtcaatgccg gtcggcagtt cgtctttggg ttcaccgcga gcataggcat
    25081 taaacatctc atcaatttgc ttctggctgg cgctatcaat actttccagc atatgtttac
    25141 gctggcggaa acgggttagc gtttgcccca gtgtattgat tcacttgaag tacgaaaaaa
    25201 ccgggagaac attggattat tcgggatctg atgggattag atttggtggg gcttgcaagc
    25261 ctgtagtaca aattttaggc gttaaccaat gaaacgcgaa agatagtaaa aaattgcttt
    25321 tgtttcattg aaaaaacgaa aaacaaaaac actataaatc atttcaatca tagcttcaaa
    25381 aaacgttcaa aaccgataac aaccaggctg tcaccaaatg actcatatca caaatcagct
    25441 catgctgagg tagcctgagt ttaacggaca ctccttcctg a
//
LOCUS       DADMEQ010000051        25411 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.051, whole genome shotgun sequence.
ACCESSION   DADMEQ010000051 DADMEQ010000000
VERSION     DADMEQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.051"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(1..2442)
                     /locus_tag="JNZ05_002119"
                     /pseudo
     CDS             complement(1..2442)
                     /locus_tag="JNZ05_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(2534..3034)
                     /locus_tag="JNZ05_002120"
     CDS             complement(2534..3034)
                     /locus_tag="JNZ05_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8565354.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            complement(3064..3780)
                     /locus_tag="JNZ05_002121"
     CDS             complement(3064..3780)
                     /locus_tag="JNZ05_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY8565355.1"
                     /translation="MVAKINLLLRLTPLLFVFAPFLAQSNMTVYPMPVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNSAQKLINAGNQRLKLLTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            4076..4852
                     /gene="exuR"
                     /locus_tag="JNZ05_002122"
     CDS             4076..4852
                     /gene="exuR"
                     /locus_tag="JNZ05_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HAY8565356.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            5197..5859
                     /locus_tag="JNZ05_002123"
     CDS             5197..5859
                     /locus_tag="JNZ05_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8565357.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            5863..6246
                     /gene="mzrA"
                     /locus_tag="JNZ05_002124"
     CDS             5863..6246
                     /gene="mzrA"
                     /locus_tag="JNZ05_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HAY8565358.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            6393..6747
                     /locus_tag="JNZ05_002125"
                     /pseudo
     CDS             6393..6747
                     /locus_tag="JNZ05_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            6785..7090
                     /locus_tag="JNZ05_002126"
     CDS             6785..7090
                     /locus_tag="JNZ05_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY8565359.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            7093..7497
                     /locus_tag="JNZ05_002127"
     CDS             7093..7497
                     /locus_tag="JNZ05_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565360.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            7487..7786
                     /locus_tag="JNZ05_002128"
     CDS             7487..7786
                     /locus_tag="JNZ05_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565361.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            7882..8364
                     /locus_tag="JNZ05_002129"
     CDS             7882..8364
                     /locus_tag="JNZ05_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY8565362.1"
                     /translation="MILSIDSNDANTAPLHKKTISSLSGAVESMMKKLEDVGVLVAHI
                     LMPILFITAGWGKITAYSGTQQYMEAMGVPGFMLPLVILLEFGGGLAILFSFLTRTTT
                     LFTAGFTLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW
                     "
     gene            8434..9420
                     /locus_tag="JNZ05_002130"
     CDS             8434..9420
                     /locus_tag="JNZ05_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY8565363.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            9713..10081
                     /locus_tag="JNZ05_002131"
     CDS             9713..10081
                     /locus_tag="JNZ05_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY8565364.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(10288..11184)
                     /locus_tag="JNZ05_002132"
     CDS             complement(10288..11184)
                     /locus_tag="JNZ05_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8565365.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYIMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFSLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGQLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            11289..11990
                     /locus_tag="JNZ05_002133"
     CDS             11289..11990
                     /locus_tag="JNZ05_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY8565366.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            12013..12177
                     /locus_tag="JNZ05_002134"
     CDS             12013..12177
                     /locus_tag="JNZ05_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565367.1"
                     /translation="MSKKSTKKRQLAKPVVAKEPARTAKNFGYEEMLSELEAIVTDAE
                     TRLAEDEATA"
     gene            complement(12389..13681)
                     /locus_tag="JNZ05_002135"
     CDS             complement(12389..13681)
                     /locus_tag="JNZ05_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HAY8565368.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGITVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGAKVSTSASAAWKAVLMALDDTAVTGNE
                     GIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(13709..14986)
                     /locus_tag="JNZ05_002136"
     CDS             complement(13709..14986)
                     /locus_tag="JNZ05_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565369.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFGVYLGFREATQGIVMNILR
                     RKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTSICSPIFGMVGCLIPAWLV
                     YKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(15261..16625)
                     /gene="tdcG"
                     /locus_tag="JNZ05_002137"
     CDS             complement(15261..16625)
                     /gene="tdcG"
                     /locus_tag="JNZ05_002137"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY8565370.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCD"
     gene            complement(16697..17086)
                     /locus_tag="JNZ05_002138"
     CDS             complement(16697..17086)
                     /locus_tag="JNZ05_002138"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HAY8565371.1"
                     /translation="MKKIIETQRALGAIGPYVQGVDLGRMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(17100..19393)
                     /gene="tdcE"
                     /locus_tag="JNZ05_002139"
                     /pseudo
     CDS             complement(17100..19393)
                     /gene="tdcE"
                     /locus_tag="JNZ05_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
     gene            complement(19427..20635)
                     /gene="tdcD"
                     /locus_tag="JNZ05_002140"
     CDS             complement(19427..20635)
                     /gene="tdcD"
                     /locus_tag="JNZ05_002140"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HAY8565372.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLALHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWIASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(20661..21992)
                     /gene="tdcC"
                     /locus_tag="JNZ05_002141"
     CDS             complement(20661..21992)
                     /gene="tdcC"
                     /locus_tag="JNZ05_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HAY8565373.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKVQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(22014..23003)
                     /gene="tdcB"
                     /locus_tag="JNZ05_002142"
     CDS             complement(22014..23003)
                     /gene="tdcB"
                     /locus_tag="JNZ05_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HAY8565374.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(23102..24040)
                     /gene="tdcA"
                     /locus_tag="JNZ05_002143"
     CDS             complement(23102..24040)
                     /gene="tdcA"
                     /locus_tag="JNZ05_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HAY8565375.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            24229..24573
                     /gene="tdcR"
                     /locus_tag="JNZ05_002144"
     CDS             24229..24573
                     /gene="tdcR"
                     /locus_tag="JNZ05_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HAY8565376.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            24829..25368
                     /locus_tag="JNZ05_002145"
     CDS             24829..25368
                     /locus_tag="JNZ05_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565377.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
BASE COUNT         6735 a         6419 c         5830 g         6427 t
ORIGIN      
        1 gaacctgaat cgactccggt aattgtgaat aagtcaccac actacaagta acatctccgc
       61 tgtaacgaat gctggcgcgt ttctttacaa cactcagtgg acacttaagg aattgctgcc
      121 cggaaagcag atagagctct ttttcgctgc cgttaatttc aagtgtaaat ccgccatctt
      181 cagctaccag taacatggcg ctgttcagcc ccacaatcgg gaattttttc accgctgccg
      241 gcagtaataa acgaccgata tagttgtagt taaagctggc agacacttcg cgatagcgca
      301 gcttgccagg caacaacatt aattcacgat tccctgcacc gttaatgatg tttgttgtcc
      361 cctgctgatt gccctggcta ctgtcgttaa cctcaacaaa accagactga aatccaggat
      421 aagggaacaa cacactgtca ctttgaccaa gctgcaaagt tcggctgcct gccacccgcg
      481 cttcgagttg cagatcctga ctctcttcat cctgttcaga gaagcctttc acatccacca
      541 gtaccgcacc cagcaaatcg tctcggtgtt caggcctccg acggttacgc caagttcttt
      601 atggctgaat gaatcgttat aaaaggtatg cgataactgc catgacgttt gatcgccatc
      661 ctgttcgcta taacgataat ccgtagacac attggttgaa tggctgttgt tattgctatc
      721 catcgttggc gtgtcagata acgaaaacga taagtacaag ccgttgtcgt cgttatcgcc
      781 accgttgtca ttacgatgga aaacaccgac tgacgtactc aaaatccagt cacgcatcac
      841 aatagcgcgg gaagcgctaa gttgtacagt ttcagagata ctttgcgctg ttgtctggcg
      901 aagatagtta ttttcaaagc tctgctgttt atcccaacgc aaactatcat cagtgttatt
      961 ttgtgtgcag gtataaccca gcatcatatt ccagccgaca aaattcagtg aaactgtcgc
     1021 ggaaatactt tcataacagc ttaaggcgtt atagctctct ctgctgtcgg ttgtacaagc
     1081 atcgccgtca gagttggtcc gataaaagcc caacgtcggc catcccggat tactccagtt
     1141 ggcctgttgc atatcacctt tgccgccgtc atcgttacgg aacacgctgg cgctgattgc
     1201 aagattcccc accccgccta aatctgccgt ccagttatta cctaactcga aagcactcac
     1261 atcatcggca ttcgccagcc ctgcgtagag ctcatattgc ggatgtaatg gcaggcgtac
     1321 tcctagctga taagctgaac tttcatcatc agaaacctgt gatgtagttt tacctgcctg
     1381 taagaaccat tgcgcatttc cgtcagtcag accgccggtt ttggtaaacg gcacaagctc
     1441 ggtgcgggtg agttggttat tttcgtagac ttttaatgcc acgctgtagc tgcctggcgg
     1501 aaaggaactg gtatcaataa attgcgaacc actattgaga taaaacgatc ccaaaagttg
     1561 ctcattacga taagcgtcaa cacgagaatt gcgcgaaagc agaaccatta ctggggttcc
     1621 ttgctggctt tgcgcctggt ttaaatagct cagtgtcgac ccgatacgca tcccatcgat
     1681 tgcaccgagt ggcagaaagt taaaggtaaa gttcccgcct tgtgcattaa atagtgtgcg
     1741 gttgtccatg cgacccgcct gcacataata acgacgtaaa aaatcataac gataatagag
     1801 atcgctgacg tcagtattac tgtcactgac atcatctgca tcataaccgt tgaaattcca
     1861 gtgtgcacca atatagctat tttctgttat tcccagcgca ccgtttccct gaatagacaa
     1921 cgattggtaa tcatcctgtg ccagaacatt gatgtcttgc tgatgtataa aagcgttaac
     1981 agtatttttg tctggattta aatataatga cttcgcatcg aaagctgaat tccattgcgg
     2041 attaataaac aacgttgccg aactttcttc atcattatag attatttcca gcgtatcggt
     2101 attcagaaaa ccacaccctg agtcagtacg tacataaccg caagccaatt caccgtggcg
     2161 agctagcggc tgcgataatt tttctttcac tatatgcgcg attttctgat cgtctacatc
     2221 cagttccagc ttgttatata gacttgctgg atcaagaaat gttacggtat caagattaac
     2281 cattgcataa tatttaccga gacttttccc tgaaaaataa acctcgatat actcctgctg
     2341 tccctgagca ataacctcaa accctgcggg aattttggtt aatgtcgcct ctgccgggct
     2401 gagactcgcc aggatcaaaa gagccagtaa ttttttatcc atggcaatcc tgaaatccat
     2461 aacaaatact aaaacagtta aaataaggga gggctacccc tccctttgtt acgtgttaat
     2521 tctgttatcc ggattaaata tcctgagaca gataaatgct tactacacca cggtagatgc
     2581 ctgtttccag aacacctttg gtggtttgag caaacatcag gtttttagcc agagaaccgt
     2641 tagttaacgc atcggaagca aagattttgg ttgcggtaaa agtggttgcc gcatcagctt
     2701 taatttcttc accaccccag gttactgtca gcggaacaat cttgctagca tccaggcttt
     2761 gaaccagttg cgctggggac actaactgca tttttacatc tttggtaacg tcgttagacc
     2821 agattttggt catcaactgg taagattgca gaccctgccc cggcagatat tgcatttcta
     2881 ctgctttcgg caacgcggtg ttatcggtct gtgtcatatc cagagcggca tcaacgttgg
     2941 ccgttacagt aatatccttt tgtactgcca atgcggagcc tgcaacagaa aacatcgctg
     3001 cgaccagaag agattttgca aatacctttt tcattactat tccttcgttt attacaatga
     3061 taattaatgg accgccagtt caaactcgcc attatctttg gtgatccagt tattatattt
     3121 gacaacgatt ttgtcgtagc cagatatgct ttcaagattt ctttcctgac cagggaagat
     3181 gtttttattt acggtctgta tcttacagtt ttctttgcta ttatttttac aataagcgat
     3241 cgttaaaagc tttaagcgtt gattccctgc attaattaat ttttgggcag aatttacttc
     3301 taacttagct ataggttgct gaggtggaac actaacaacg attccccaaa ttaagttaac
     3361 tgttaactgc atagagagat cttttttatc tgtggcaacg tcatcaatac tgggaaccgc
     3421 ttcaaattta acgcgataat ttttctcttt ttgctctggt tccatcgcaa caaaacgtac
     3481 ggttttgcta ctaccggctg gtaaagcgaa tttaggtggc ataacaacca gatgatttgc
     3541 atcaccggat ttaatctcaa cttcattttc ctgaggcgtc gatggattat cgatacggaa
     3601 tactgtcgcc ttgatgtact gaacttcatt acttttagat attacgcgaa cgttgccttc
     3661 accctgacta ttaatggaaa caggcatagg ataaacagtc atatttgact gtgccaaaaa
     3721 aggagcgaac acgaataaaa gcggcgtaag ccgtaataag agattaattt tcgccaccat
     3781 ataattttga tcttacaata tcgtttcaaa aaagttggtg tattagagat gtagatttat
     3841 tgtaagtcaa tataaaaaag catttttagt taacattaaa tttaaaggct gggaattaac
     3901 ttactgaaat ttagtagctt tcacaatgac gatacttatt ttttaggaat ttataaatat
     3961 tcacaatatt aatttaatac attaattcaa tcatcaaatt tatatatgac caatggcata
     4021 cccatgaata aagtggtatg ataacttcgt ccgcattcca ctccgggaga cgcatatgga
     4081 aatcactgaa ccacgccgtt tgtatcaaca acttgctgct gacctgaaag agcgcattga
     4141 acagggcgtc tatctggtgg gtgataaact gcctgccgaa cgctttattg ccgatgaaaa
     4201 gaacgtcagc cgaacggtag ttcgtgaagc catcatcatg ctggaagttg aaggctatgt
     4261 ggaggtgcgt aaaggttcag gtattcatgt ggtttccaac cagccgcgtc atcagcaggc
     4321 ggctgacaat aatatggaat tcgccaatta cggtccgttt gagttgcttc aggctcgcca
     4381 gctcatcgaa agtaatattg ccgagttcgc ggcaactcag gtaacgaaac aagacatcat
     4441 gaaactgatg gccattcagg aacaggcgcg cggcgaacaa tgcttccgtg attccgagtg
     4501 ggatttacag ttccacattc aggtagccct ggcgacgcag aactccgccc tggcggccat
     4561 cgttgaaaaa atgtggaccc agcgtagcca taacccgtac tggaaaaaac tacacgaaca
     4621 cattgattcc cgtaccgttg ataactggtg tgatgaccac gatcaaattc tcaaggcgtt
     4681 aattcgcaaa gatcctcatg ctgctaagct ggcaatgtgg cagcatctgg aaaacaccaa
     4741 aatcatgtta tttaacgaaa ccagcgacga tttcgagttc aatgccgacc gctatctgtt
     4801 cgccgaaaac ccggtagtgc atctcgatac ggccaccagc ggcagcaaat gaaattcctg
     4861 tccgacaggc gctgcaatgc gcctgtttgt ctcgtcggta agcgaaaagt ataaagtgtc
     4921 agcctgtgta aatcctctcg ccaccctccc ctgcaatcag caaaatcaga ctccacggac
     4981 atgtaatttt gataacgaac aacgttgacc tttgttacaa ttagattcaa tttgaattta
     5041 tgcttttgaa tgctttctta tctcacgttt taacagggaa tagttcaggc tgtgttgatg
     5101 tatcaaaccc gcagaacata ccaaaacagc aataacattg cggtagtgca tcttttaaaa
     5161 ccagcgtggc gttaaccgat tcaccaggaa taatgaatgg aacttttgac ccaattgctg
     5221 caagccctgt gggcgcagga ttttgaaacc ctggccaatc catcgatgat tggcatgttg
     5281 tattttgtct tgtttgtaat tttgttcctt gaaaatggct tgcttccggc ggccttttta
     5341 ccgggcgaca gtttactggt attggtcggc gtgttgattg cgaaaggcgc gatgggttac
     5401 ccgcaaacga ttctgctgct gaccgttgcc gccagcctcg gttgctgggt cagctatatt
     5461 caggggcgat ggctgggcaa tacccgcacc gtacaaaact ggctatctca tttacccgcg
     5521 cattatcatc aacgcgcaca ccatcttttt cataaacacg gtttatcggc gctgttaatt
     5581 ggtcgcttta ttgcatttgt cagaacactg ctgccgacga ttgccgggtt atcagggctg
     5641 aataacgcgc gctttcagtt cttcaactgg atgagcggtc tgctgtgggt attgatcctg
     5701 acaactctgg gttacatgct cggcaaaacg ccggtatttt taaagtacga ggaccagctg
     5761 atgtcatgcc tgatgctgct cccggtggtg ctgctggtgt ttggcctggc aggttctctg
     5821 gtcgtgttat ggaaaaagaa atatggaaat cgggggtaag ggatgcaaat acctcgcatg
     5881 tcgcttcgcc agctagcctg gtccggcact gttttacttc tggtcggcac gctgttgctg
     5941 gcctggtccg cggttcgcca gcaagagtct acgctggcga ttcgtgccgt tcatcaaggc
     6001 acaacgatgc cagacggttt ttcgatctgg catcaccttg acgctcatgg cattcctttc
     6061 aaaagtatca cccccaaaaa cgacactctg ttaattacat ttgactccag cgaccagagc
     6121 gccgccgcaa aagcggtcct cgacagaaca ttgccccatg gctacatcat tgcgcagcag
     6181 gacaataaca gtcaggctat gcagtggctg acccggttac gggataactc tcatcgcttc
     6241 ggataacttc ctgttatccg aaacatatca ctcactttgg tgatttcacc gtaactgtct
     6301 atgattaatg aggcggtggt taacactacc gcttcgttgt ctgaacaatc gttcctttgt
     6361 aataggtccg aatcacaatg gaaggttcaa gaatgaaata ccgcatcgct ttagctgttt
     6421 ctctctttgc tcttagtgcc ggtagttatg ccactaccct gtgtcaggaa aaggagcaaa
     6481 atatccttaa ggagatcagc tatgccgaaa aacaccaaaa ccagaatcgt attgacggtc
     6541 tgaataaagc cctgagtgaa gtccgggcca actgttcaga tagccagctg cgtgccgatc
     6601 atcacaagaa aatcgcaaag cagaaagatg aggtggcgga acgccagcaa gatttagccg
     6661 aggcgaagca aaaaggcgat gccgatagaa cggaaactgg cagaagcgca ggaagagctg
     6721 aaaaagctgg aagcgcgcga ctactaactc acaatagtca ctacttactc acctggagaa
     6781 aactatgtcg aaagaacaca ctacggaaca tctgcgtgct gagttgaaat ccctttccga
     6841 tacgctggaa gaggtgctta gctcatctgg cgagaagtcg aaagaagagt tgagtaagat
     6901 tcgtagcaaa gcggagcagg cactgaaaca gagccgttat cgcctgggtg aaaccggtga
     6961 tgccatcgcc aaacaaaccc gtgtcgcggc ggcgcgtgct gatgagtatg tgcgtgaaaa
     7021 tccgtggacc ggcgtgggaa ttggcgctgc aatcggtgta gtgctcggcg ttctgctgtc
     7081 gcgtcgttaa ttatggcgga cactcatcac gcgcaagggc ccggtaaaag cgttctgggc
     7141 atcgggcagc gaattgtttc catcatggtt gaaatggtgg aaacacgtct gcggctggcg
     7201 gtggtggagc tggaagagga aaaagctaat ctctttcaac ttttactgat gctgggcctg
     7261 acgatgcttt tcgctgcatt tggtcttatg agcctgatgg tgctaattat ttgggcggtt
     7321 gatccgcaat atcgcctgaa tgcgatgatt gccaccaccg tggtgttgct gctactggca
     7381 ctgattggcg gtatctggac gttacgtaaa tcgcgtaaat ccaccttgct gcgccataca
     7441 cgccatgagc tggcaaacga tcggcaactg ctcgaggagg agtcccgtga gcagtaaagt
     7501 cgaacgtgaa cgacgtaagg cgcaactgct tagccagatc cagcaacaac ggctggatct
     7561 ttccgccagt cgtcgtgaat ggctggaggc aacaggcgct tacgatcgtc gctggaatat
     7621 gctgctaagt ctgcgctcct gggcgctggt tggcagtagc gtgatggcga tctggacgat
     7681 tcgccatcct aatatgctgg tccgctgggc cagacgcggt tttggcgtat ggagcgcctg
     7741 gcgtctggtt aaaacgaccc tcaagcagca acagcttcgc ggttaacttc cctctggccg
     7801 gagcaattcc ggccttatcc ctcaaatttt ttgaatattt ttgacagttt tccttgctaa
     7861 caatcatcat tcaccacgtt tatgattctc tccatcgaca gcaacgacgc taataccgcg
     7921 cccttgcaca aaaaaacaat cagcagcctg agtggcgcag tggagagtat gatgaaaaaa
     7981 ttagaagatg ttggtgtact ggtagcgcac attttaatgc cgattctgtt tattaccgca
     8041 ggctggggaa aaatcaccgc ttactctggt acccaacaat atatggaagc aatgggtgtc
     8101 ccgggtttta tgctgccgct ggtgattctg cttgagttcg gtggtggtct ggcaatccta
     8161 ttcagtttcc tgactcgcac cacaaccctg tttactgcgg gctttacgct gctgacggca
     8221 tttttatttc acagcaactt tgctgaaggc gtcaactcgc tgatgttcat gaaaaacctg
     8281 acaatttctg gcggattcct gctgctggca attaccggtc cgggcgcgta tagcatcgac
     8341 cgcctgctga ataaaaagtg gtaagcacgc gctatactta acgataaaaa acgaggagga
     8401 agctcctcgt ttttgctatt ggaggagaga aaaatgggtc aactgattga cggcgtctgg
     8461 catgacacct ggtacgatac caaatctacc ggcggtaaat ttcaacgttc agcttccgca
     8521 tttcgtaact ggctcactgc cgatggcgca cctggcccca ctggcacagg cggttttatc
     8581 gcagagaaag atcgttatca tctctatgtt tcactcgcct gcccgtgggc gcaccgcacg
     8641 ctgatcatgc gcaaactcaa aggactggaa ccgtttattt ccgtttccgt agtgaacccg
     8701 ctgatgctgg aaaacggctg gacctttgat gacagttttc cgggagcaac cggagatacg
     8761 ctctatcaac atgaatttct gtatcagctt tatctccacg ccgatccaca ctacagcgga
     8821 cgagttactg ttcccgtgct gtgggacaaa aagaaccaca ccatcgtcag caacgaatca
     8881 gcagaaatca tccgcatgtt caataccgcg tttgatgcgc tgggcgcaaa agcgggtgat
     8941 tactacccac cagccctgca aacgaaaatt gacgaactta acggctggat ttatgacacc
     9001 gttaacaacg gcgtgtataa agctggtttt gccaccagcc agcaagccta cgacgaggcg
     9061 gtggcgaaag tgtttgaatc gctggcgcga ctggagcaga ttttaggcca gcatcgttac
     9121 ctgaccggca accagctaac cgaagccgat attcgcctgt ggactacgct ggtgcgtttt
     9181 gatccagtgt atgtgaccca cttcaagtgt gataagcacc gcatcagcga ttacctgaat
     9241 ctgtatggct tcctgcgcga tatctaccag atgccgggga tcgccgaaac tgtcaatttc
     9301 gatcacatcc gtaatcatta cttccgcagc cataagacca tcaaccctac ggggattatt
     9361 tcaattggtc cgtggcagga tctcgatgaa ccgcatggac gagatgttcg cttcggttaa
     9421 aaaaaggcac cttacgaggt gcctctgata attagctaag tgcatgttgt tcatgtcgga
     9481 tgcggcgtga acgccttatc aaacctacaa agcgtttata ttcaatatgt tacaatgacc
     9541 gcgtaggcca gataagcgta gcgcatctgg cgattttgcg cttgccatca gtcttagcgc
     9601 gccttcacgg cactttatta attccttgaa cgaatattta ctgccatttc attcacgtct
     9661 attcttaatt tgctgcttta aaaacaagtg attgagcaaa attgaggcaa aaatggactg
     9721 gtatctgaaa gtactaaaaa attatgtcgg tttccggggt cgtgcgcggc gcaaagagta
     9781 ctggatgttt attctggtca acatcatctt tacgttcgtg ctggggctgc tggataaaat
     9841 gttaggctgg caacgcgctg gtggcgaagg catcctgacg acaatctacg gtattctggt
     9901 gtttttaccg tggtgggcgg ttcagttccg ccgcctgcac gacaccgacc gctcggcgtg
     9961 gtgggcactg ctattcttaa tcccgtttat cggctggctt attatcatcg tctttaactg
    10021 ccaggcgggt acgccaggcg aaaaccgctt tggaccagat ccaaaactgg aacaagaata
    10081 atgattaagc accttgtaaa aggtgcttca aattgttgac aaagtgcgct ttgtttatgc
    10141 cggatgcggc gtaaacgcct tatccagcct acaaattgcc aaaattcaat atattgcagt
    10201 gactgcgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgtc agcagtcaga
    10261 agcaccttgt gaaaggtgct taatagatta ttttccagcg aacaactttg gaatttcccg
    10321 cagacaccag gatttcgctt cccccatact gtcacgacgc caggccataa taatatcgat
    10381 ctcgctggtc gattccgggc tgacgacacg caactgccct tccgcaatat ctttttcgac
    10441 catcggatac ggcatcgtcg ccacgccaag ccccgccagt aatgcctgac gtttatcttc
    10501 aatcgtgctc accgttaagc gcggctgttt gtccagcagc tgtacggtca acaccgggcg
    10561 ctcacgagcg gtatccgcta ccgcaattcc acgatatttc acacgcgtca cttcagataa
    10621 cggttccggc tcctgatgaa tcgggtgatc aggcgcggca acgtagacgt tcattaacgt
    10681 atagagcttg cgcgagttga tctccgacga ggaacgaaaa tgcatatccg gcgcgataac
    10741 gatatccgcc cgcccctgct ccagccgttc ccacgccccc gccagcactt ctgtgatgat
    10801 tgccagttgg gtattggctt ttgccgccag tttgtcgatt aacgagaaaa aggcaggtgt
    10861 cggtaccagc gcttcggtca caatggtgag atgcgtttcc caaccgcgcg cgagagcttc
    10921 cgcatcagta gtcagtttat ctgcggcttc cagcaaaacg cgccctcgct ccagcaacat
    10981 cctcccgaca ttggtgaatt tggtgcgatg gcccgagcgg tcaaacagca ccacatccag
    11041 ctcttcttcc agtttttgca tgatgtagct aagtgcggaa ggcacgcgtc ccagctcatc
    11101 cgctgccgcc gcaaaactgc cccggcgatc gatcgcatcc ataacccgta gtgcttccag
    11161 cgttaatgcc ctttctttgg ccatttcgtt ctcattcagg aaatttgaac ataccgggca
    11221 gaatatctgg ctaacaatgt agcgtccagc cccttaccat aaaaggaagt aaagagaggt
    11281 caagaattat gattactacc cgaactgcca ggcagtgtgg acaagcagac tacggatggt
    11341 tgcaggcccg gtatactttt tcctttggac actacttcga cccgaaattg ttaggctatg
    11401 cctccctgcg tgtgcttaac caggaagtgc tggccccagg tgccgccttt cagccgcgaa
    11461 cctatcccaa agtcgatatt ttaaatgtga ttctggatgg agaagcagag tatcgcgaca
    11521 gcgaaggcaa tcatgttcag gccagcgccg gagaggcgtt gctgctctct acccagccgg
    11581 gtgtcagcta tagcgaacac aatctcagca aagacaaacc gttaacgcga atgcagcttt
    11641 ggctggatac ctgcccgcag cgagagaatc cgctgattca aaagctggcg cttaatatgg
    11701 acaagcagca attaatcgcc tcgccagagg gtgcgatggg aagcctgcaa ttacgccaac
    11761 aagtgtggct gcaccatatc gtgctcgaca aaggcgaaag tgcgaatttc cagttgcatg
    11821 ggccacgcgc gtatttgcaa tcgattcacg ggaaatttca tgcgcttacg catcatgaag
    11881 agaaagcggc gctaacctgc ggtgatgggg cgtttattcg tgacgaggct aacattacgc
    11941 tggttgccga ttccccactg cgcgctttgc tgatagattt gcctgtctag ttgtttttac
    12001 agggagatga tgatgagtaa aaaatcgacc aaaaagcgcc agctagcgaa gcccgtggtg
    12061 gcgaaagaac ctgctcgcac cgccaaaaat tttggctatg aagagatgtt gagcgagctg
    12121 gaagctatcg tcacggatgc tgaaacgcgt ttagccgagg atgaagctac cgcgtaaaga
    12181 caggttgccg gatgcggcgt aaacgcctta tccggcctac aaaacacaac aacatcaaca
    12241 aattaaacac cccgtaggcc ggataagatg cgccagcatc gcatccggca tcaggtacaa
    12301 aacacaacaa catcaacaag ttgagcaccc cgtaggccgg ataaggtgcg ccagcatcgc
    12361 atccggcaca atactcaaaa cctggcgttt atctggcctt gctcgccata atctcgataa
    12421 tctgccgatc cgtttgctgc atcgaatggc ttgctaacgc acacaggttg gcaatcgact
    12481 gctcaacatc atgcgccacg atcccttcat tgccggtcac ggcggtatca tccagcgcca
    12541 ttaacaccgc tttccacgca gccgaagcac tggtcgaaac cttcgcacca tcgcaaatca
    12601 tgccgctgac atcgccgatc atactgctga tcgccatcga gatagtttca taacgcccat
    12661 ccaccagcca tgccatcccg gcggcggccc ccattgctgc ggtcgttgcg gcacaaagtg
    12721 cagacaaacg cggtaactgg ttatggatat aaattgcgct caaatgagaa agcatcagcg
    12781 cacgcgccag tcgttcatca tccgctccga agtgttctgc tacaaccacc acaggcattg
    12841 ttgcggtgat cccctggtta cccgagccgg agttactcat agccggaagc gtagcgccgc
    12901 ccatacgcgc atcggatgcc gcgctggtac gaatcacaat ggatgaagag agatctttcg
    12961 ccagcaaacc gcgctcgcac tgtttttcca gcgtcgcgcc aatatgcagc ccccacttac
    13021 cgctcaaacc ttcctgcgat aacgcacaat ttagcttcgc ggaatcgaga ataaagcgga
    13081 tcgccgcaaa tgggacttca ttgacgaact tcaggatctc agccagcgtc gttctggaaa
    13141 gcaccgtcag cggcgactct tgctcgccct ctgccacaca cgcctgctgg gtaaacacca
    13201 caccattgtg cgtctcaata tgcacaatgt tggtatgccc gccgacgatg gtgacacacg
    13261 cccacttctc accgttccag actttggcgc gtgagaagag gatttcattg caaggttcct
    13321 ggatcttaac agagactttc cccgccgcca gcagtgcttt ggcatcggca attgcctgcg
    13381 cagttgcgtc tttcagcact tccagcccgg cgttggcatt tccacctaac gcccccagcg
    13441 ccgccgcaat cggcagcccc accattcccg tgccgggaac ggtgatgccc agaccgttct
    13501 tcatcagatt tggcgaaacc caggcttcta cacgttcaac cggaccttcc agttctgccg
    13561 cagcaaccgc cgccgccagc gccagtgaaa tcggttcagt acatcccagc gccggtttta
    13621 cttcctcctg aacggcgagg atgtaacgct gccataacgg atttaaagtc gaatcaaaca
    13681 tattaaaaac cttaaaattt caggtaaatc aagaaaatgc caggaacaga gaaacacaaa
    13741 gcaacaaacc agtgacgata atcaggtaca gagacatccc tttgtatttg tgcaatgccg
    13801 gtactttgta aaccagccac gccgggatca ggcaccctac catgccgaaa atcgggctac
    13861 agatagaggt gaagctcaac accggtgcgt tcagtacgat ggcgctccag gccagcaaaa
    13921 tggcgaaaat catgatgccg cgctgaacaa gattttcgtt aatcttctcg gcaggcatct
    13981 tgcgacgcag gatgttcatt acgatccctt gcgttgcttc gcgaaagcct aaatagacgc
    14041 caaagaatgc agtcatgaca gcaaagatat tgagaatgac gctgaccact ttcacccacg
    14101 ctgcgccgtc accgctaata aactgtgcgg caatcgccag cgtgaacgac acggcgtaga
    14161 aaaagacggt gacaaacaaa atgccaaacg cgatattcat tgcccgcaat gctttatgcc
    14221 gcgccacttc aattgatttt tcccgcgagc gataagagat caccatcggg cttaacgtct
    14281 ggataaacag aatcgacgtc agggtaaacg gcagcgtaat aatggcgttt ttcaccagca
    14341 gccccagcgg cggtagcgaa ccgacgttat acagatgcca cattcccacc atcgacacgc
    14401 ccagcgcagc gactaccagc agcttggtca gcaccatgtc ggtcgaaatt ttgaataaca
    14461 atttctcgcc gcgtgaagag atcgccacca gaatgcaaat cagtaccaga ccataaaagg
    14521 gactgtctga cagcaaccct tccgtcacgc cgaaggtatg cagataggaa gcactatcgt
    14581 tggtgatggc ggtggaatag acgaacatcc aaatcaccag cattacgaaa tagagcgcac
    14641 ctaacaggat gccccagttt ttacctaaat aaccgctaat gacactcggg taatctttac
    14701 attctggtga ttctgccagc gtattaataa acaaccgctg aaacagatac attgccgggt
    14761 aaccaatcac cgatgagagc aaaaataccc acaatcccat caaaccgacc tgcaccggga
    14821 gaaaaacaat ccccgcgcca atcgccatcc cgatactcat aatcacccag ccggtgtcag
    14881 tactgtcgaa tttgatcgct tctcgccact cgctctcact cattccagca cgacccgccg
    14941 gggtcgaagc gtctgcaatg acgcctttat tcgatgcaat ttccataatt tctcgctcaa
    15001 tattttgtag ggtttattat ttttttccga gccgcatcaa ggcgatatgc ggtctacgtt
    15061 tttgcaggcg agttattaga ataaagaaat gatacgcgcg aggtcagaga aaatcttcac
    15121 aatcaagact ttaaaataac ggagatggat aagaattttc tactaaatta atcgcaagag
    15181 aatattcatt ctctatttgc ggcagatcac aaaaaaaagg tgcacatttg tgcacccaag
    15241 gatgaaagct gacagcaatg tcagtcgcag accactttaa tggccagtcc tccgcgtgat
    15301 gtttcgcggt atttatcgtt catatctttg ccggtttcat acatcgtctc gatcacttta
    15361 tcgagtgaaa cacgcggtgc cgaggtgcgg cgcatcgcca tccgcgcggc gtttactgct
    15421 ttcacggcat taatggcatt acgttcaatg cacgggattt gtacctgtcc ggcaaccgga
    15481 tcgcaggtca gcccaaggtt atgctccatc gcgatttccg ccgcattgca tacctgcgcc
    15541 ggactgccgc ccagtagttc agttaaccct gccgctgcca ttgaacaggc cacgccaatc
    15601 tccccctgac agccgacctc cgcgccagag atggaggcgt tcattttata cagcgcgcca
    15661 atagcccccg ctgccagaaa atagcgggca attgaccgct cgtttaccgg acgacggaac
    15721 ttatcgtaat aagccagcac tgccggaata atgccgcacg caccgttagt cggtgccgtt
    15781 accacgcgcc cgccagctgc gttttcttca ctaaccgcca gcgcgtacat gttgatccag
    15841 tcgatgacat tcatcggatc gttagagatg ttatcgctag aaaccagctg acgacgcagc
    15901 gctacggcac gacgcggcac attgagcgga ccaggcagca cgccttcagt gttcatcccg
    15961 cgttcaatac cgtcatgcat cacttgccag atacgggcaa accctgcgtc aatttccgct
    16021 ttgctgcgca gcgccagctc gttgtgcatc atcaggccag atatagacag gccgttgtaa
    16081 tcacacattt tcagcagttc acctgctgag tggaaatcgt aaggtacgga cgtttcgaca
    16141 tcgtgcgaca ggccgaagtg ttcttcttcg acaataaacc cgccgccgac agagtaatag
    16201 gttttactta atagcgcttc ctgtcccttc caggcagtga tccgcattcc gttctcatgg
    16261 cgaggcaaca tttcgggatg gaagataatg ttctttgcta caggaaaatc aacaatatgc
    16321 gcaccagatg ccactggcag ccgcccgctg cgcgttacta actctataaa tgcagggatc
    16381 tcatcaatga caacatcctg cggactgttt cctgccagtc ccatgataat ggcgacatcc
    16441 gtggcatggc ctttgcccgt cagtgacaac gacccgtaca gatcgaccac aatatggctc
    16501 gtcgcggtta ataagccgct actttccagc cgatcaataa aactttttcc ggcattcatt
    16561 ggccccacgg tatgcgaact ggagggacca atcccaattt tgaaaatatc gaatgcacta
    16621 atcatatcca caccctcgga ttgccgttca gtgaagtgga gcggaacgac cttgcgaccg
    16681 tcccgctcac gaggctttac gcactacgta ctgcgatagc ttcaatttcc agcttcacat
    16741 ctttcggcaa acgcgcaacc tgcacacagc tccgggtcgg gtaggtcgcc tgatgctcat
    16801 cgaagaactg cttatagact tcattgatgg tggcaaaatc attcagatcg gtgatgaaca
    16861 cggtcatctt gatgatatcg cccacgctca gcccggcagc aaccacgatc gctttgacgt
    16921 tttcgaggct taaacgcgcc tgatcttgca catcagccgg gatctcaccg gtctgcgggc
    16981 aaaccggaat ttgcccggag gtgaagacca ttctgcctaa atcaacgccc tgaacataag
    17041 ggccgattgc gcctagggca cgttgcgttt cgataatctt tttcatacat cctccggcgt
    17101 cagagcgcct gggtaaaagt acgtgaaata acatcctgtt gctgttcacg ggtcagtgcg
    17161 ttgaagcgca cggcgtagcc agagacacgg attgtcaggt taggatattt ttccgggtgc
    17221 tcgatggcat ccagcagcat ttcccgattc attacgttga cgttgaggtg ttgaccgcct
    17281 tcgacatccg cttcgtggtg gaaataccca tccagcaggc cgacaaggtt ggttttacgt
    17341 actggatctt ctttgcccag cgccgcagga acgattgaga aggtgtacga gatcccatct
    17401 ttggcgtagg tgaacggcag tttcgccacc gacgtcaatg aggccacggc acctttgcgg
    17461 tcacgaccat gcatcgggtt agcgcccggc gcgaacggtg ttccggcgcg acgaccgtcc
    17521 ggcgtattac cggttttctg gccgtacacc acgttagaag tgatagtcag aatcgactgg
    17581 gtagggacgg cgttgcgata ggttggcagc gctttaattt tcttcataaa gcgttcaacc
    17641 aggtcgcagg caatgctgtc tacgcgctcg tcgttgttgc cgtactgcgg atattcaccg
    17701 tcgatttcaa agtccaccgc caggccgttt tcgtcacgga ttggtttcac gcgggcatat
    17761 ttgatggcag acagggagtc cgtcgccacc gacaggcccg cgatgccgca tgccatagtg
    17821 cgatagacat cacgatcgtg cagcgccatc agcgaagctt cgtagctgta cttgtcgtgc
    17881 atgtagtgaa tgatattcag cgcgctgatg tactgcaccg ccagccagtc catgaagtga
    17941 tcgaggctgt ccatcacttt gtcgtaatcc agcacgtcgt ccatcagcgg tgctgttttc
    18001 ggcccgacct gaatcttcag cttctcgtcc accccgccgt taattgcgta gagcagcgtt
    18061 ttcgccaggt tagcgcgtgc accaaagaac tgcatttgct taccaatcac cattgggctg
    18121 acgcagcagg caatcgcgta atcgtcgctg ttgaagtcag tacgcatcag atcgtcattt
    18181 tcatactgca aggaagaggt gacgatcgac acctgcgcgg catatttttt gaaggcaatc
    18241 ggtaattctt ccgaccaaag aatggtcagg ttaggttccg gtgccggccc catagtgtgc
    18301 aggtgtgcaa atagcggaag gagtttttgg tcaccagcgt acgaccgtcc agccccatcc
    18361 cgccgatcac ttccgtcgcc cagattgggt cgccggagaa cagcgaatca aattccggtg
    18421 tacgcaggaa gcgtaccata cggatcttca tgatgaagtg atcgatcagt tcctgtgcct
    18481 gctgctcatt gaggacgcca gctttaaagt cgcgctcaat gtagatatcc aggaacgatg
    18541 ccgtgcgccc cagcgacatc gcgccgccat tttgcgattt cactgccgcc agataagcga
    18601 agtagagcca ctgcaccgct tcctgtgcat tctgcgccgg gcgagagata tcaaagccat
    18661 atttcgccgc catttcctga atctgcaaca gcgcatgacg atgctctgcc agctcctcac
    18721 gcagacggat ggtggcttcc agatcctcgc ctttttccag acgagactgg agatcggcaa
    18781 attgcagttc gcgttcacgc accagataac tgatgccata cagcgctacg cggcgatagt
    18841 caccgataat gcgcccacga ccatagccat ctggtaaacc ggtcagcacg ccatatttac
    18901 ggcagcgcag catatccggt gagtaaacat caaatacgcc ctggttatgg gttttacgca
    18961 gatcggtaaa cagatattca aattcactgt ccatttcacg gccataggcg tggaatgaac
    19021 ttttaatcat attaatgcca ccgaacgggt gtagcgcacg tttcaacggc gcatccgttt
    19081 gcaggccaac aattttttcc agcggctggt taatatatcc cgcatcatga gcggtaattg
    19141 tggtggcaat attggtatcg aaatcaaccg gcgcgtgggt tgcgttttcg atacggatgc
    19201 cttccattac tttttcccac aattccgtgg tggcaggcgt cgcttcggcg agaaaagatt
    19261 catcgccttc atacggtgta tagttatgtt gaataaaatc gcggacatta atttcgcttt
    19321 tccagtccgt acctttaaag ccaagccatg cgtcagcgta cagcttatcg ctggtatcaa
    19381 tatctacctt catgaaaaat aatctctcta caatacttca actaaattat gcaaattctg
    19441 cgggcgcgtt aactttacct aaatgaatgg catccaaagc aatcattttt tcttcgttag
    19501 tcggaataac gacgcaaatg acatgcgcat tttcactgga aacaattcgc tcgccaaaag
    19561 agttagatcg attattcatt tctgtatcaa tctctacgcc taatacagcc aaatgttcca
    19621 tgaccagacg acgaattaaa cttgaattct ctcctattcc gccggtgaat ataattccat
    19681 ccagccgacg taatgaagct gcatgtccgg caatatgacg ggcaattcgg tgaacaaagg
    19741 ttttaattgc cagttgcgcg cgttcgtgac cttcatgcca ggctttttcc agaacacgta
    19801 aatccgaaga aagaccggaa atacctaata atcccgactc tttattcact acgcgttcca
    19861 ggtcacccag gctctggttg gtttggctgg cgatccagga catcgcacca aagtcgacat
    19921 cgccactgcg ggtacccatc atcaagcctt ccagcggcgt cattcccatt gaggtgtcaa
    19981 cactctgacc gttgcgaact gcgcagattg acgcgccatt gccaagatgc gccacaacca
    20041 ggccggagtc atcttccgcc agattcagca gcgaatgtgc gcgctgggaa acatagcggt
    20101 gcgacgtgcc gtggaaacca taacggcgta cacctaactc ttcataatat ttccacggca
    20161 ggccgtataa ataagcttcc ggagccatcg tctggtggaa actggtatca aatacggcca
    20221 cctgagttac gcccggaaat aattgctgcg ccgattcaat accacttaaa tttgcgtaat
    20281 tatgcagggg tgccagtgga gaaacgcgac ggatattatc aatgacttca tcggtaataa
    20341 tggcggactc ggtaaaaata ctgccgccgt gagcgatgcg gtggccaatt aaggccacgc
    20401 tgtcatttaa attccgtttt tccagttcaa atgcaattgc cttcaatgca ccttcgtagc
    20461 tgtggagagc cagcggtgct ggctctcccc catttacgga taagaatgca ttttccgagt
    20521 taataccgtc ggcaatacct gacattaata cttcacagtc gctggcatcg agtacggaaa
    20581 acttaatcga agacgaacca cagttaataa ccaaaacaac cggaaattca ttcatctctt
    20641 ttctcatcct gagttacgga ttaaaacagt ttgtatacga tgttcaggat ggtcagcaga
    20701 ccaatcacgg taacaaacac gttatccaga cgaccacggt atttcgccag agacggcgct
    20761 ttacggatag catacatcgg caacaggcac agcagggatg cgataatcgg tgcgcccatg
    20821 gcttcaatca ggtcgaggat gttcgggttg gcgtaggcaa caacccaggt ggagcccatg
    20881 atgaagatca tgctgagagt attcagttta cccagcgaca ctttggtttt gtcaccttta
    20941 taaccgaact tcagaatcag accattcaag ccttccagcg tccccagata gtgaccgaag
    21001 aaagatttga agatagccac gagtgcgatg atggaagccg catattccag tgtaatcgcg
    21061 aacgttgttt tcgtaccggt catggacgca aagtggttag ccagataaga aagcactgga
    21121 atattctgca ctttggcttc cgccatgttg gccggagaca gagtaaacag gcagctaaag
    21181 gcaaagaaca tcaccactgc aaccatcagc atgctggcac gagaaatgat ttgggaacat
    21241 ttacgttcgg tgaagtcgcg accgaagtct ttctcatact cttcacgttt agaaaccacg
    21301 aaggaagaga cgattggcga gaagttaaag gagaaaacca tgatggaaat ccccagccag
    21361 acagtgatca ggataccgtc atgaccggtt aacgacagcg aaccgaggtc aacctggtcg
    21421 ataactgcag agttccagta agggatcagc gacagagaaa tcagcaccag gctggcgata
    21481 aacggccata ccaggtagct catcacttta accatcagat ccttaccaaa ccagatgacg
    21541 aaagccatca gcagcaacag gaacagcgcc acaaagccgc gattcagcgg tgcaaatccg
    21601 agctggtttt cccagaacgt cataaaggta ttggtaatag taacgccata aatccacagc
    21661 agtgggcaaa tcgcgaagaa gtacaggaac gtgataacca cgccgccagt tttaccaaaa
    21721 tgctcttcca ccgtttccgt aatgttgccg gaagggttag agccagaaag acacagacgc
    21781 gccagcgccc ggtggcaata aaatgcgatg gggtatgcca ataccaacat cagaagaatc
    21841 gggatcagtc cgccaaaacc tgcgcggata gggaagaaca gcaccccggc gccgattgcc
    21901 gtaccaaaca agcctaacgt ccatgtggta tctgatttac gccaggacga ttgtttcgtc
    21961 tggctggata caatgctatc tgaagtactc atatcctatc ctcaacgaat taattaagcg
    22021 tcaacaaaac cggtgatttg agagacgcga gaaagatcga tattgccgcc ggaaataata
    22081 ctgacggttt ttctgttttg aatatattgg tctaatttac cgcttaataa tgcagcacat
    22141 gccagagcgc ctgcgccttc ggtgacgact ttattgcgct gaattaaggc aatcatactg
    22201 tttctgattt cgtcttcgct gaccagcacg atgtcatcga ctaattcacg aacgatttcg
    22261 taagttaaat tacccgggcg ggagacatca caaccatccg ccagggtgcc ggtagttcgg
    22321 tgcgtggtta tttctccgga gtggaaagaa gccgccatgc cgtgaacgtt ttctgactgt
    22381 acgccaataa cacgaatggt cgggttaata gatttaattg ccaccgcaat accagcaatt
    22441 aaaccaccac caccaattgg cacaatcacg ttatcgacat catagagatc ttccataatt
    22501 tccagaccaa tcgttccctg gccagcaatc actttcggat catcgtaagg tgggataaaa
    22561 atacgacctt ccatttcgac aatttcgctc actttagcga tagtgtcgtt gaagttatca
    22621 ccatgcagaa cgacttctgc ggagtagtcg cacgttgccg ctactttgga ttttggcgca
    22681 ccttttggca tcaccacttt accgtcgata cccagcatcg cgcaggagag cgaaacccct
    22741 tgcgcatggt tgcccgcaga acaggccacc acgcctttgc gtttttccgc atcggtcagt
    22801 gaacttaatt tattaaatgc gccacgaatt ttaaatgaac ccgtacgctg catattttca
    22861 aacttcagga atatttcacc tttgcaacgt tcactaaaat agttggagcg aggcatgcct
    22921 gttttataaa ttcgcccagc cagtcgttgt ttcgcttcaa taatatcatc aatagcaacc
    22981 ggcagatcgt atgtaatatg cattataaaa cctcttcgcc tgaattaaat atgtaggtaa
    23041 ccgtaaccga cacctgcaaa acagacaggt ggattattta tgtttaaata aaaacaaata
    23101 actaaccaac ttctattaat tgccttcgtc tacacccatt ataagatgaa tactctttgg
    23161 ctaattccac caaaaccgat gctgcttttt taatacgata atttttcgac cataccgcgg
    23221 catattgtgc cacaggtaat gtttcttcaa ccggaatagt aataaattga ttagaaccaa
    23281 aaggtgacgt catatcacaa ggaattacag ttaagaaatc agcattgaga acaagattat
    23341 aaattgtcac gactgagtcg gttttaacga tgttttcaat actgatgcca tttctttgta
    23401 acgtagtaag cagttcgctg tagtacccca tattagtttg tggcaacacc cactgttcgt
    23461 tcttcaacga ctccagcgtg gtggtgccgg tgcatgttcg ggacttactg gctaccagca
    23521 caaactcgga ctcgaacagc ggctcaacat gtaaatcctg aagcttcatt tctgcactta
    23581 acgtaccaat cgcaaaatcc agtcgaccgt cgcggattgc cggtaagaac gaagacagtt
    23641 gcgcttcata catagaaacc tgcgctttcg ggaacacctc tttgaacttg ttgatcatcc
    23701 ctgacataaa agtaaaacca atcaatgaag gaaaaccaaa tgagacttcc accaccgcct
    23761 cagaagacat accgcttatc tcattaacca tatttttcat ttcacgggta atggattcgg
    23821 aacgggagag taacaattga ccggcaggtg ttaatgttac accggtattt ttccgcacca
    23881 ctaattccac accaaaataa tcttcaatat cgttaatgat tttactgacg gccggttgag
    23941 ttaaccctaa ttcttttgca gccgagccga tagagccact tctaatgact tcctgaaaga
    24001 ctaccaggtg ctgcgttttc ggaagaagaa tagtgctcat aacgacctac gttaattacc
    24061 tcattgacgg catgaagtgt atcaaaatga aataaacagg atatgtgcga ccactcacaa
    24121 attaactttc attactttcc aaggtatcgt tattacaatt aaataccttt aaatatcaac
    24181 aagttaaagc ataaaaatca gcataaaccc tgatttctat caaaaaaaat gacggggata
    24241 accatatttt atggcgataa catcattcgt tatgtggtta atacaaaaaa ggggctgaga
    24301 ccatatttca agcaattacc ggataattac caggcaaaat ttgagttaaa tttaatgagc
    24361 aagttctcaa attttattat aaataaacca ttttcagcga taaatactgc ggcacgtcac
    24421 attttttcac gctatttgtt ggagaacaaa catttatttt atcaatattt taaaatttcg
    24481 aatacaggta ttgatcatct cgaacaattg attaacgtca actttttctc ttctgacagg
    24541 acgtcatttt gtgaatgcaa tcgttttcca taaattcttc tcccctcata ggcgacgaat
    24601 agcattttgt gttgaggatc acaaaacgaa taattgctgg tcgccgcgat aaggtcagac
    24661 gaagtcaaca agggggattt tcactgtgct ttacatcagc gtaggacaca gaatgttgct
    24721 tcgtttactg taacgccggg taaatgagcg ttttttgata gtgcgtaagt gtcgtgcgag
    24781 gaaaataagc atctattatt gtttgtcatg acgacaaggc aggcatcaat gaaaggcttc
    24841 ccaattgcac atatttttca cccttcaatc ccgccaatgc acgcagtggt taacaatcac
    24901 aatagaaata ttgattattg gacggtaaaa agaaagtttg cagaaattgt ctccaccaat
    24961 gacgttaata aaatttacag tataagtaat gaactgcgga gagtattatc tgcaattact
    25021 gcattgaatt tctatcaagg cgatgttcct tctgtcatga tccgaatcca accggaaaat
    25081 atgagtccat tcatcataga tatttctaca ggagaacatg atgattatat catacaaaca
    25141 ttagatgtag gcacttttgc accttttggt gaacaatgta cttgctcagc cgtcaataaa
    25201 aaagagctgg aatgtattaa ggagacgatt tctaagtatt gcgcaaagtt cacccaaaaa
    25261 gaagccatct taacaccccc taggcacttt aataaaacct gcattacttc agattgttgg
    25321 caaattctct ttttttcacc cgatcatttt aataatgatt tttattgatt atttataaag
    25381 gataaaataa tgttccctgt ttcatcaatt g
//
LOCUS       DADMEQ010000052        25154 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.052, whole genome shotgun sequence.
ACCESSION   DADMEQ010000052 DADMEQ010000000
VERSION     DADMEQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.052"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..315
                     /locus_tag="JNZ05_002146"
     CDS             <1..315
                     /locus_tag="JNZ05_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8565378.1"
                     /translation="ILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRCACCGHTAK
                     ENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQA
                     TA"
     gene            complement(359..4976)
                     /locus_tag="JNZ05_002147"
                     /pseudo
     CDS             complement(359..4976)
                     /locus_tag="JNZ05_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(5069..5716)
                     /gene="rnt"
                     /locus_tag="JNZ05_002148"
     CDS             complement(5069..5716)
                     /gene="rnt"
                     /locus_tag="JNZ05_002148"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HAY8565379.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5819..6226)
                     /gene="gloA"
                     /locus_tag="JNZ05_002149"
     CDS             complement(5819..6226)
                     /gene="gloA"
                     /locus_tag="JNZ05_002149"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAY8565380.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(6307..7404)
                     /gene="nemA"
                     /locus_tag="JNZ05_002150"
     CDS             complement(6307..7404)
                     /gene="nemA"
                     /locus_tag="JNZ05_002150"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HAY8565381.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGRAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(7441..8040)
                     /locus_tag="JNZ05_002151"
     CDS             complement(7441..8040)
                     /locus_tag="JNZ05_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY8565382.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8143..8382
                     /locus_tag="JNZ05_002152"
     CDS             8143..8382
                     /locus_tag="JNZ05_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HAY8565383.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8431..9327
                     /locus_tag="JNZ05_002153"
     CDS             8431..9327
                     /locus_tag="JNZ05_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAY8565384.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGMLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9408..9929
                     /gene="sodC"
                     /locus_tag="JNZ05_002154"
     CDS             9408..9929
                     /gene="sodC"
                     /locus_tag="JNZ05_002154"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HAY8565385.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(9930..11912)
                     /locus_tag="JNZ05_002155"
                     /pseudo
     CDS             complement(9930..11912)
                     /locus_tag="JNZ05_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(11809..12696)
                     /locus_tag="JNZ05_002156"
     CDS             complement(11809..12696)
                     /locus_tag="JNZ05_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY8565386.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIHAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALVQGHAGAMALCV
                     TQYHCHVSGADGCLLFESG"
     gene            complement(12699..12935)
                     /locus_tag="JNZ05_002157"
     CDS             complement(12699..12935)
                     /locus_tag="JNZ05_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HAY8565387.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            13136..13570
                     /gene="slyA"
                     /locus_tag="JNZ05_002158"
     CDS             13136..13570
                     /gene="slyA"
                     /locus_tag="JNZ05_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HAY8565388.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(13617..14084)
                     /gene="slyB"
                     /locus_tag="JNZ05_002159"
     CDS             complement(13617..14084)
                     /gene="slyB"
                     /locus_tag="JNZ05_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HAY8565389.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            14358..15467
                     /gene="anmK"
                     /locus_tag="JNZ05_002160"
     CDS             14358..15467
                     /gene="anmK"
                     /locus_tag="JNZ05_002160"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAY8565390.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            15565..15894
                     /gene="mliC"
                     /locus_tag="JNZ05_002161"
     CDS             15565..15894
                     /gene="mliC"
                     /locus_tag="JNZ05_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HAY8565391.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGFSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            15953..16609
                     /gene="pdxH"
                     /locus_tag="JNZ05_002162"
     CDS             15953..16609
                     /gene="pdxH"
                     /locus_tag="JNZ05_002162"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HAY8565392.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            16738..18012
                     /gene="tyrS"
                     /locus_tag="JNZ05_002163"
     CDS             16738..18012
                     /gene="tyrS"
                     /locus_tag="JNZ05_002163"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAY8565393.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            18074..18940
                     /gene="pdxY"
                     /locus_tag="JNZ05_002164"
     CDS             18074..18940
                     /gene="pdxY"
                     /locus_tag="JNZ05_002164"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HAY8565394.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVK
                     AANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVN
                     NVEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMR
                     QPVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRI
                     AKPEHYFSATKL"
     gene            complement(18984..19589)
                     /gene="gstA"
                     /locus_tag="JNZ05_002165"
     CDS             complement(18984..19589)
                     /gene="gstA"
                     /locus_tag="JNZ05_002165"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HAY8565395.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(19695..21197)
                     /gene="dtpA"
                     /locus_tag="JNZ05_002166"
     CDS             complement(19695..21197)
                     /gene="dtpA"
                     /locus_tag="JNZ05_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HAY8565396.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(21808..22443)
                     /gene="nth"
                     /locus_tag="JNZ05_002167"
     CDS             complement(21808..22443)
                     /gene="nth"
                     /locus_tag="JNZ05_002167"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAY8565397.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(22443..23138)
                     /locus_tag="JNZ05_002168"
     CDS             complement(22443..23138)
                     /locus_tag="JNZ05_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HAY8565398.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(23142..23762)
                     /gene="rsxG"
                     /locus_tag="JNZ05_002169"
     CDS             complement(23142..23762)
                     /gene="rsxG"
                     /locus_tag="JNZ05_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HAY8565399.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFGQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(23766..24824)
                     /gene="rsxD"
                     /locus_tag="JNZ05_002170"
     CDS             complement(23766..24824)
                     /gene="rsxD"
                     /locus_tag="JNZ05_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HAY8565400.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(24825..>25154)
                     /locus_tag="JNZ05_002171"
     CDS             complement(24825..>25154)
                     /locus_tag="JNZ05_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY8565401.1"
                     /translation="ARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAE
                     PEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQ
                     QKVVNED"
BASE COUNT         6198 a         6507 c         6580 g         5869 t
ORIGIN      
        1 atactggatc agggctggta tgaaatgcgc cgccagcttg agtacaagca gctctggagt
       61 ggcggtcagg tgcttgctgt tccgccagcg tacacaagcc agcgttgcgc gtgctgtggt
      121 catacagcga aagaaaaccg cctgtcacaa agtaaattca gatgccaggt atgtggatat
      181 acagcgaacg ccgatgtaaa tggcgctcgt aacattttag cggcggggca cgccgttctt
      241 gcctgtggag agatggtgca gtcaggccgc tcgttgaagc aggaacccac cgaaatgatt
      301 caggcgacag cttgaacgta gcagggaacc acgtctttca gggcgtggag gatgtcaact
      361 atccccaatc cagcccttgt ggcgaacttg aaaatcccgc ctcacgcagc agcgtaaaca
      421 tcggcgtttg ccggaccgga agatcattca cttctgttag cgtaaagcgc agccttggtt
      481 cgcgacgcag tgcggtggtc agcgcctgga aaatctctgg ggcgagtaat tcctcctttt
      541 cctgccagac cagcattttt ttgccgcctt gcgccagata gagtaacaat ttgccgccag
      601 aaacgaccac cagcgcaccc gcccgacgcg ttggtaccag cgctgccgga tgtgcaggcc
      661 agggcagaag atttccccac acattagccg gatcgttggc ggagagcgcc actggtgtat
      721 agtggcgtgt ttgcgtggct tgtgtcgcca gatcgcgcaa tcggtcaata gtcagacgtt
      781 cagcgaattg cgcgccaccc agaccttcta caaaacgacc tcgcataatt cgcccggagt
      841 cttccatact tcgacaaagt gtttgcatcg atggaaaccc gccagggata ttttcggcta
      901 tcaccgcctg acgactgatg atgccgtagc ggtcgagcat attttccgcc agcgccagca
      961 tcctttcggt atcgtttagt ggctccacct gcaataacga ccagcgtcca gccagatttg
     1021 gtgtgttgta agatacccgc ggcgagacgg gttgtgcata gacaggacgc ccacgacgag
     1081 cccggtgact gcggcgagtt gaggtgcgtg cgttggaact gctgcgggtg agggcgcgta
     1141 acggtgccca aatatcgctg gtaatgacgc cttgccagac cagcgcccat aacgcctctt
     1201 gcagtgccga ggaatcaacc gattcgccga ttttatcgcg tatccgttgg ctgatttgtt
     1261 gtgcaaacca ggctcctccg tcagccagaa cagcgattat cgcttgctgc agcgccgaac
     1321 gattcgcctg atccgcttcg gcgggagtga acgattctgc ggcatatttc tgtagatgca
     1381 gtgccaccag gccgtcatct tcacccagct tttttttgtc ccgaccagat aaccgctccg
     1441 gttgccagta attcatcgag catttctggc gaatagtcgc gtacgcgggc aggcaggatc
     1501 tggctttccc agagtgaggc gggtaaaccg actccggcaa gctgttcgat cacccgcatc
     1561 acgccatcta cgccctcata aacgcctggc gatgttgagg caaagagcgc cgggctacca
     1621 tcggtggcgg gtaatacgcc ctgacgttcc agcagcaatc gcgcataggt cgtggctgca
     1681 acgggacgcg tcgcttctct ggcggcttgc agcgagcgca aacgcagacg acgaaatact
     1741 tcatcgctca cccagatgtc ttgttgcaga ttcatcacca gaccctgttc acgcagttgc
     1801 tgaagctgct cttcgacaat ggcaataccg agactaaact catgagccag ttgttcagcc
     1861 gtgaccagag catgagcccg gagatagcgc agaaagaggt cgcgaagcgg gtaactgact
     1921 ctatgtaaat aaatctctgg caatgactct ggtagtcgta cgccgagggc atcacgcagc
     1981 ctggcggcat catccataca ggcaagacgc tcctgtccgc taatcatcgc agggaagatt
     2041 cgttttactg caagaagatt ttccagatac gacgcaacct cttcactgct gcctgtatgc
     2101 cgttgcgcca ggtcttcaac ggtcattggc cccagttcgc gcagcaggtc gaacaatcct
     2161 tcttcacctt tcgctcttct gccaggagcc agtcgttgca actcttcttc cacctggcga
     2221 atgacctgcg ggtcgagtaa ttcccccgga tcgacctgtc cgagtagatt gcgcagtaac
     2281 tcgctgtcca gcgacagtac ggatgcccgg cgctctgcca gcggggcgtc gctctggtac
     2341 ataaattccg cgacatagcc gaacaataaa cttgtggcga aaggcgaggg cgtagtggtc
     2401 gttacatcgg atatttgaat ttcgccaccg ttcaggcgac gcatcaaacg ttccagtgcg
     2461 ggaagatcat aaacatcttg cagacattcg cgtagggttt cgagaatgac cggaaaatcc
     2521 ggatatccct gagcgatttc cagcaactga ctggcgcgca gccgttgttg ccaaagcggg
     2581 gtgcgatggc ccggcgtgcg gcccggcatt aataatgccc gcgcggcgca ttcgcgaaaa
     2641 cgggcggcga aaagtgccga gctgcctacc gcctcgcgga caatttgcag caacttttct
     2701 ggttcaaaca aaaaaatcgc ggcgtcgggc aatttaccat cagtgtcagg aatgcgcgca
     2761 acaatgccgt catcactggc gaccacagac gcgtcagcgc cccatagggc atgtattcgc
     2821 ccggcaatcg ccagcgccca gggttcatgc acccgtcttc catagggaga gtgcaaaata
     2881 atacgccagt cgccaatttc atcacggcac cgttcgagga ccagatggcg actccccgga
     2941 acgatgcccg tcgcgttgcg ttgctcgtca atcaacccct gaatattggc gttcgtattt
     3001 tcttctgcca gccacggggg aatagtcccg gaaaagaacg cgccatccgc cagtaaatga
     3061 agaaaatcgc cgatcatctc gcctaattca gccggacgtc cgttaccttc accacgccag
     3121 aaggggagct gggcagaacg acccggagca ggagtcacaa tcacctgatc gcgggtgatt
     3181 tgctggatcc gccatgaggt agcgccgaga gtgataatgt cgttcacccg cgactcatat
     3241 accatctcct catccagttc acccacccgc cgcgaaccgg ccttttcttc accttcgggt
     3301 aataacacgc tatacatgcc acgatccgga atggtgccgc cgctggtaac cgccaacaat
     3361 tgagcgccag gtcgggcggt caatatcccg gtctccctgt tccagaccag tttggggcga
     3421 aaagcagaaa aatcgccaga gggatagcgc ccggaaagca tatccagcgt ggcgtcaaaa
     3481 acacgtcttg gcagatcttt ccacggtgcg gcacggcgta cgcgggagta ccattcgtct
     3541 acctgtaatg catccatcgc cgcggcggca acggtttgct gcgcaaggac gtcgagagga
     3601 ttatgcggtg gtgtcaggtt ttccagcctg cctgcgaaca tacactctac aatgactgcg
     3661 gaatcgacta aatcacgccg ggtacggggg aaaaacagcc ctttagatac accgccaacc
     3721 tgatgtccgg cgcgaccaat gcgttgtaac ccactggcaa cagaaagcgg cgttgcctcc
     3781 tgaatcacca gatccaccgc gcccatatca atccccagtt caagactgga ggttgcgacc
     3841 acgcagcgta attcacccga tttcagcgcc tgttcggtga ttgctcgttg ttctttagag
     3901 acggagccgt ggtgtgagcg ggcaataaaa acgtcgctac tttgtacacg gttagaggtt
     3961 gcgccggagg tcgactcgaa atgggccgca tcaacggcga tagacgggga acgctgtaag
     4021 cgtgcggcgt aaagctcatt taatcgtgcc gtcagttttt ccgccagccc gcgtgaatta
     4081 gtaaagacaa tggtcgagcg atggcgcaac acttcatcaa ggatacccgt ttcaatatat
     4141 ggccagatgg agccttcccg gccggcatgg ctgtcttcgc cggtgccgct ggcgaccgat
     4201 gagacatcat ccatattggc gaccggtacg acaattcgta tctgcggatg gcgcattgcg
     4261 ggcgggttga ctaccgtaac cgggcgatcg ccaccaagaa atgctgccac atcgctggct
     4321 gagcgcacag tggcagaaag gccaattcgc tgtgctgagg tgtggagcag cgcatcgagc
     4381 cgctccagac ttaacgccag atgcgcacca cgtttactgc ccgctaccgc gtggacttca
     4441 tcaataatta ccgtttcgac gccgtgtagc gtttcgcgcg cgcgggaggt cagcatcaga
     4501 tagagtgatt cgggtgtggt gatcagaata tccggcggat tacgggtgag tttgctgcgt
     4561 tcctgtgcag gcgtatcgcc agtacggatc cctacgcgaa gattgacttc cgtttcgccg
     4621 cgccgccgcc gttcatcggc aatacccttc aacgggatct gcaagttgcg ctgaacgtcg
     4681 gtgcccaggg cttttatcgg tgaaatatag aggatgcgtg aggttttacg cttatgcgcc
     4741 tcgcgggtat cttcgccgcc ttcgcggaag agccgatcga gggcgtagag aaatgctgcc
     4801 agcgttttcc cggagccagt cggtgcaatc accagcgcat gttcgcctcg cgccgccaca
     4861 tgccaggttt gcgactggac agcggtcggc tgtttaaagg cgcgaagaaa ccagtcgcgg
     4921 gtcgccggtg aaaacacgtc cggcaggagc gatgaagggt ctggattatc tgccattaac
     4981 gctattcccc gtgaatatca tgcttgatgc gtggaatagc ctcactatag atgcctgaat
     5041 cattgcatgt catcaggcat cgactcgatt acacctcttc ggcggcaggt agcggccagc
     5101 ctcccagacg tttccagcgg ttgacgattt cacaaaacag cacagcagtg cgttcggtgt
     5161 cgtacagcgc ggagtgcgcc tgggtgctgt cgaagtccat gccagcggtc tggcaagcct
     5221 ttgacaatac ggtttgtccg agtgccagcc cggccagtgc agcagtgtca aaagtggcga
     5281 aagggtggaa cgggttacgt ttcagcgagg cgcgttctgc ggcggccatc ataaagctgt
     5341 gatcaaaatt ggcgttgtgc gccaccataa tggcgcggtt acagccgctc gctttaatac
     5401 ctttacgtac aactttaaaa atttcgtgca gcgcctcgta ttcgctgacc gcgccgcgat
     5461 cgggatcgtt cgggtcaatg ccgttgaagg cgagggcttc tggttgcaaa tttgcgccga
     5521 caaatggttc gacgtggaaa tgtaatgtgg tgtccggcat cagccagcct tgttcatcca
     5581 ttttcagggt gatggcggca atctcaagca gcgcatcggt tttggcgtta aatccggctg
     5641 tttcaacatc gatcacaaca ggataaaaac cacgaaaacg gtcgcacaga ccggtaagtt
     5701 gagcgttatc ggacatctga ttctcttaat acggataaat tgcagcgcgc attatgacaa
     5761 atattacttg tcgatgcagt aaagatgcgg gcgcgatgag ttcacgcccg gcaggagatt
     5821 agttgcccag accgcgaccg gcgtctttct cttcgattaa ctcaattttg taaccgtccg
     5881 gatcttccac aaacgcgata accgtagtac cgccttttac cggacccgcc tcacgggtca
     5941 cgttaccccc gttttgacgg attttttcgc acgcttcagc ggcgttatct acgctaagcg
     6001 cgatgtgacc ataagcagtg ccgagttcgt atttatccac gccccagttg taggtcagtt
     6061 caatcaccgc ttcttcggtt tccgggccgt agccaacaaa cgccagtgag tatttgtatt
     6121 ccgggttttc gctggtacgc agcagtttca tgcccagcac tttggtataa aaatcgatgg
     6181 agcgttgcaa atcgccaacg cgcagcatgg tatgaagaag acgcatcttt ttatcctcaa
     6241 aatggctaac agattcaaaa tgttgtttta gtatagcggc ggctttacgc cgctcgcaat
     6301 gttggattac aacgtcgggt aatcggtata gccttccgcg ccgccaccgt agaaactttc
     6361 ggcacgctgt gggttaagct cagctttgag ctgcaagcgg gcgaccagat ccgggttcgc
     6421 aatccagtca cgaccaaatg ccaccgcatc aattaaccct ttgccgatca gcgtttcagc
     6481 tttttctact gtgtatgcac ctgcgccgat aatcggaccg tggaaacggg cgcgtacttt
     6541 ttcgcggaac gcatcagtat acggttcacc ccccgcccaa tctggttctg acatatgcag
     6601 ataagcaatg ccgcgtttac ccagttgttc aatcagatac agtgcatcgg cttcttcatt
     6661 cgggccgtta tctgtgttct ggaaagtacc gattggcgaa acgcgaatgc caatgcgatc
     6721 ggcaccccat tcttcaatcc cggcatcgac cacttccagt accaaacgtg cgcgattttc
     6781 cacgctgccg ccgtactgat cggtacgatg gtttgaagaa ggggaaagga actgatgcag
     6841 caaataaccg tgagcagagt ggagctctac cagatcaaaa ccggcttcac gcgcgttagc
     6901 aatggcctga cggaaatcat tgacgatacc cggaatctct tccagttcaa gcgcacgcgg
     6961 catggatgtt tcaacgcgga tcgcccgacc attttcatcg cgcagagaag tacgtgttcc
     7021 cgcgctaagt gctgaaggcg ctaccggtgc ctgaccgcca ggttgcaggc tggcgtgaga
     7081 aatgcgtccg gtgtgccaca gctgcaaggc catatgacca ttttcagcat gaacgccagc
     7141 ggtgattttt ttccatgcgg caatttgctc cggactatgg atgccaggcg cacctgcata
     7201 tccttttgcc tgggcagaaa tttgcgtggc ttcactaata atcaaaccgg cactggcacg
     7261 ttggcgatag tattccgcca tcaacggggt aggaatgtca cccggttcaa tactgcgcag
     7321 acgcgtcagc ggtgccataa aaatacggtt tgccgccgtg atcgcgccca ctttcagtgg
     7381 ggaatacagt ttttcagatg acataaaggc tcccggtcgt ctggtgataa aaaatgcctg
     7441 ctaaacggca ggcgtcgcaa taatgttttt tacatgggcc agcgcgtttt ccagtggctc
     7501 gaaactgcgc gaaattttgg cctgcagatt cgcgccaagc catagtgcgt aaagcacttg
     7561 tgcctgttgt agcggttcgc cacaaaaggt taaacaatgg ttctcacggc cattttccag
     7621 agcctgcgag agcagggcga tcacgccgcg agcaccttta tccatcgcgc tgcgcatatc
     7681 ttctgacaga tcgcacactt cggcagagag ttttactgtc aggcaaccac tgatggttcc
     7741 atgttggcaa aactggttca gtgtttgctg gtaataagcc agtatgcggt cgcggtagtt
     7801 accttcgccg gattgcagca actcagccag tcgctggtga tatgcggcgt aatgacgctc
     7861 aagcatggca acgccaaacg cttctttaga gcgaaagtag tgatagaagg accctttcgg
     7921 cacttcagcg gtttttagta attcgcttag ccccatcccg gtgaatccac gttgcaggca
     7981 aagttgctcg cccgtcgcca agagatgttc gcgagtatca tgttcggtgt gtttgttcat
     8041 gcgttggagt gtagtagacc agtcggtcta gtgcaagcaa agttgctact ttggacatta
     8101 acagcttcaa taatggtaat aatcttatct gaggagggcg aggtggcgga gcaattagag
     8161 ttctttcccg tccagagccc gtgccgggga atttgccagt ctgacgaacg cgggttttgt
     8221 cgcggctgtt ttcgtagtcg ggatgagcgt tttaactgga ataaaatgag cgatggcgaa
     8281 aaacaagagg tgctgcgcct ttgtcggcag cgattaatgc gcaaattacg cgcaaataag
     8341 ccagcgtcat cagacgaacc ggagcaacca tcactctttt gaacctaatc ttgcgtatac
     8401 tcctctcata tctgataaga ggaagcgatt atggttcagc gtattactat tgcgccggaa
     8461 ggcccggagt tttcccgttt tgtgatgggc tactggcgat tgatggactg gaatatgtcc
     8521 gcccgccagc tggtcagttt tattgaagag catctggatc tcggcgtgac caccgtggac
     8581 catgctgata tttatggtgg ctatcagtgc gaagcggcgt ttggcgaggc actgaaactg
     8641 gcacctcacc tgcgtgaacg gatggaaatc gtcagtaaat gcggtatcgc gacgaccgcg
     8701 cgtgaagaaa acgtcattgg tcattacatc actgaccgca atcacatcat taagagcgcc
     8761 gaacagtcgc taattaatct cgcgaccgat catctggatc tgctgttaat ccaccgacca
     8821 gacccgttaa tggatgccga tgaagtggcg gacgcgttca aacatctgca tcagagcggc
     8881 aaagtgcgtc attttggcgt atcgaacttt acgcctgcgc aatttgccct gttgcaatca
     8941 cgtctgccgt ttacccttgc cactaatcag gtggaaatat ccccggtgca tcagccgtta
     9001 ctgctggatg gcatgctcga ccaactacaa caactgcgtg ttcgtccgat ggcgtggtcc
     9061 tgccttggtg gtggtcgtct gtttaatgat gattatttcc agccgctgcg tgatgaactg
     9121 gctgtggtgg cggaggagtt aaacgcgggc tcgattgaac aggtggttta cgcctgggta
     9181 ttacgtttac cgtcgcagcc gctgccaatt atcggctcag gtaaaattga gcgcgtacgg
     9241 gcagctgtcg aagcagaaac actgaaaatg acccgtcaac aatggtttcg tatccgtaaa
     9301 gctgcactgg ggtacgacgt accgtaagca cttttaggaa tagccgccgt tcaaaaatgt
     9361 gtcactggtt tacacttatt caggaatgca caatgaacgg aggtcctatg aaacgtttta
     9421 gtctggccat tctggcgctg gttgttgcga ccggcgcaca agctgccagt gaaaaagtcg
     9481 agatgaacct cgtcacgtcg caaggggttg ggcagtcaat tggtagcgtc accattactg
     9541 aaaccgataa aggtctggag ttttcacccg atctgaaagc attacccccc ggtgaacatg
     9601 gcttccatat tcatgccaaa ggaagctgcc agccagccac caaagatggc aaagccagcg
     9661 ccgcggaatc cgcaggcggg catcttgatc cacaaaatac cggtaaacat gaagggccag
     9721 aaggtaccgg gcatttaggc gatctgcctg cactggtcgt caataatgac ggcaaagcta
     9781 ccgatgccgt catcgcgcct cgtctgaaat cactggatga aatcaaagac aaggcgctga
     9841 tggtccacgt tggcggcgat aatatgtccg atcaacctaa accgctgggc ggtggcggtg
     9901 aacgctatgc ctgtggtgta attaagtaat tacgaggcca gcgtaccttg cggtggtgct
     9961 tgctcaagtt gcgaaagcga gcagtacagc cgccagacaa ttgccgccag ctcacgggcg
    10021 gcaggttgat gatgacgggc aaggctgtcg caaatccgct gtaattcttc aagtgtggcc
    10081 gccagtgatt tttgctgaac gccacgctca ctcatcactc cccgcaacag tgaaatacag
    10141 ttatcccgta ctcgcgataa cggatcggaa cgcgattccc agtcgcgcaa ttgccagaca
    10201 acatgagaac agttcagcag cactacaccc cagcgtaata accaacggcg agccagcgca
    10261 tcctggctgt tactcaactg actgacgtga tgataagtga gcgattcaaa ttcgctttca
    10321 ctcagtgttg gatggcggct tagctgatcg acaaaatccc ggcgcagcgc gcgaatatgg
    10381 cggcggcttt tacgagcatc cgatcctgga cgcagaatgg cgaacgctag ccaagccagc
    10441 gcaacgccaa cgatttttgc cagattatcg ttaagaaaat cagcaaaatc atacaccggg
    10501 ggattagtga cagcgataaa cgaacccata aaaacaatca gttgccccca cagtgcggca
    10561 aatttgggca tctgcaattt aagaagctgc attgtcgcca gcagtggaaa gagaaacagt
    10621 aaaaattgcc acagatcgct aatctggacc atcaggccaa atttgaccac aaagctgaat
    10681 agcgaaagta acaccagcgt gcgcatcagc agtgacaacg acttaaacgg tgctgcgacg
    10741 gcggagtaga gtacgcagct aattgctgcc agcgttaatg cattggcacc ggcatcccat
    10801 tgcgatgcaa tactccatgc gccaatcatc atcaacgtac aaaatgtacg cagcccgctc
    10861 cacatagctt cggcgttgtc ggtatgacga gccagagcgg atgtccgtgc gagtctggca
    10921 tgatcatcta caccgctttg cagacgatgt aattcctgac tactttgcag ataaaggcgg
    10981 caaaaataac gtagtcgctg ccagaaggcg acgtgccggt agtcggcgac gttggtcggg
    11041 cgtagcgggg cgataatacg tgcgacggtg taagcatctg tttgcgaact ggcgagcgcc
    11101 gtcagtaatt gttcgagaat ttctcgcgtg gcacctggcg gtgagggcca gttgagcaac
    11161 atacgtcgca ggctggagat gacactggtc atacgtaatt gctggtggag tagcgcatta
    11221 aggcgcgcgt tttgctggcg aaaacgatag tggctccaga aagcctggat acgcagcaaa
    11281 ttcatggtca gtatctgccc aatcaccccc tcatgtgctg cacgaatggc atcgtttgtt
    11341 tcaggctgcc agagtaaact ggcatgttcc agtaatcggg cgtgcatgtt tttcaatgca
    11401 gttaaaaggg cagtagcatc ggaactgcta ggcaggatca tcatcatcat gccgccgcac
    11461 aaaataccga ctatcacctc gcaaacgcgc gcctgagcga tatcccacag ctggctggcc
    11521 tcagtaatat taaccatcgg aaaggcgatg atggcagccg tgtagcccgc cagttgaaat
    11581 gcatacgcga cgttattcgt gaagtgcgca caggcctagg tacaaaagcc aagccacgcc
    11641 gacatgctca atagaaaaaa ccacggctca ttgagcgtat gtccggcaag aagcagtgcc
    11701 gcaatggctc ccagtaaact gccagcgatg cgtccgaggc ttttgctgat gacaccgcca
    11761 acggtgggaa agctaaccac tgcagccgag gtcatcgccc aatagggttc atccagattc
    11821 aaataatagg caaccgtcag cgccagacac atggcaatgg tattgcgtaa cgcataacgc
    11881 cattgcgcca gcgtggccct gaaccagcgc aaagcggacg ggaacgcgtt gggcgagacg
    11941 aacccagggg acgttaggtt tcacatccgg aataaggctt gagtcacttt caacgctctg
    12001 atcataaatc gcccgaccga tactggaaac gtgaccctgt aacgttttgt tatcactata
    12061 gagggtaatt tgtgctggtg caccttcgcg gatatggcgc aacttggttt cttcaaaata
    12121 accaatgacg taaaacgagt ggctgtcgac aagggcaaac agcggtttcc cggtgtcagc
    12181 gtaatcgccg atgcgggtag tgaggttagt cacccatcca ctcaccgggg cgcgaatttc
    12241 cgtttgcgcc agttgccatt gtgtctgttt cagcgtcgcc tgtgcggcat taacactggc
    12301 ctgcatcgct ttaacattga ggttagcggt atcgagttct tcggcaaaaa taaaattttg
    12361 tgagagatga cgacgacgat tcgcttcgtt attggctttt gcgaggtcag attgcgcttt
    12421 cgccagctga gcctgtgcgt tcagctcggc gatctgaaag ggcgttttat cgatcgtgag
    12481 cagaagttcc cccgcgttaa ccagttggtt gtcttttata ttcagctcaa caatacgtcc
    12541 cgacacctgt ggggtgatag aaacctgttc tgcgtgtatt ttcccgtcgc gtgtccacgg
    12601 agattgcatg taataattcc acagccacca tccggcaaga accgcaacta tcgctacaat
    12661 gattgttgag aaatacttaa ttgttttaat tgacatagtt accacgcaat cagtattgcc
    12721 agagcaaggc aaacgcaaat cgcaaacagc gataaatcca ttaacaaggg atgccagatg
    12781 tcaccggcgt agatccagcc acgaagcaga cggtgtacga caagccagat gacgaatcca
    12841 aacgcgaatg ctttgaaaaa cggaggaaaa tagacggacg caccgaacac cagatcttgt
    12901 aagggcaatc ctgttgcatt gagcataaac ttcacaaagt agattccttt acgaccgaat
    12961 atattgcgtt agattaataa atattcttta agtgcgaaaa atttacgcgc aatttctgaa
    13021 aaatatgtct gacggtaacc aaatgcagca atacatttgt tttagcaata caattgctgc
    13081 acactattct aaaagccgca taatatctta gcaagctaat tataaggaga tgaaattgga
    13141 atcgccacta ggttctgatc tggcacggtt ggtgcgcata tggcgtgctc tgatagacca
    13201 tcgcctgaaa ccgctggagt taacacaaac ccattgggtt acgttacaca atatccatca
    13261 gttacctcca gaccagtcgc aaattcaact ggcaaaagcg attggcatcg agcagccatc
    13321 actggtccgt actctggacc aactggaaga aaaagggtta atttcgcgtc aaacttgtgc
    13381 cagcgatcgt cgggctaaac gtattaaact gacggaaaag gcagagccac tgatcagcga
    13441 aatggaagct gttattaaca aaacccgcgc ggaaatatta catggcatct ccgcagagga
    13501 actggagcaa ctgattacgc tcatcgcaaa acttgagcat aatatcattg agttacaggc
    13561 caaagggtga aatgaagggg gcgtgtggtc agtaacctga ccacacgcaa acttatttag
    13621 cgcggagaaa cggtcacctg actgccattg ctggccagta cgacacgttg gcccggagag
    13681 aaacgagtgt tgccttgttt ctgtaccacc atgatggtat taccatcgtc tttacgaatt
    13741 tccagctcga caccctgcgt tttgttcatt gcactctgta cgccctgacc agctacgcca
    13801 cctgcaacag cgcctgctgc agtagccaga gaacgcccgg ttccgccacc aacagtattc
    13861 cccaggaaac caccaagaac agcaccgcca attgcaccga taacgttgga atcatcaccg
    13921 ccctgaatct gtaccggacg tacgttaacg atggtgccat agctgacatt ctgtacttgt
    13981 ttcgcttcag aagcggtata aacatcccct gacagggtgt cgttattaac acaaccgaca
    14041 agagacagac ctaccattga aacaaccaat acgcgtttaa tcactgaaaa atctcctgtt
    14101 caccatgaaa cgccacgcaa gcatccctca tggtcaaagt atatggcata tgtgaatcca
    14161 tggttacatg ttctaccaaa tatgagcaaa tcataatgaa agtaggatca accaattata
    14221 aacaaaacat tcatgatgat tattgatttg aagataatgt tttactacca acggtaaaaa
    14281 tcggtcattc cgcatggtat tcacgaaaag tttatgtttg agtggtgcgc aaacgtcaga
    14341 ttgaaaaagg atgaactatg aaatcgggcc gctttattgg cgttatgtca ggcaccagcc
    14401 ttgatggtgt tgatgttgtg ttggcgacaa ttgatgaaca ccgggtcgca cagctggcaa
    14461 gcttgagctg gccgatcccg gtgtctctga aacaggctgt actggatatt tgccagggcc
    14521 agcagcttac actttcgcag tttggacagc ttgatactca actcgggcga ctttttgctg
    14581 atgcggtcaa tgccttgctt aaggaacaaa acctgcaggc gagagatata gttgcgatcg
    14641 gctgtcacgg tcaaaccgtc tggcatgaac cgacgggcgt ggcaccacac actttacaga
    14701 ttggcgataa caatcaaatt gtggcacgca ccggaattac ggttgtcggt gattttcgcc
    14761 gtcgcgatat tgccttggga ggacaaggcg caccgctggt acctgcgttc catcatgcct
    14821 tgctggctca cccaaccgag cgacgaatgg tgctcaatat tggcggcatc gccaatctgt
    14881 cactgctcat tcctgggcag ccggttggcg gctacgatac cggtcctggt aacatgctga
    14941 tggatgcctg gatctggcgt caggcaggta aaccttacga taaagatgcc gagtgggcac
    15001 gggcgggtaa agttattctc ccactgctgc aaaatatgct cagcgacccg tatttctcgc
    15061 aacctgcacc gaaaagcacc ggacgcgaat actttaacta cggctggctg gagcgccatt
    15121 tacgccattt tccgggtgtt gatccccgtg atgttcaggc cacactggca gaactcaccg
    15181 ccgtgaccat ttctgaacaa gttttgttga gcggtggctg cgaacgattg atggtatgtg
    15241 gtggaggtag tcgtaatccg ctactcatgg cgcgtctggc ggtattactg ctaggcacag
    15301 aagtcaccac caccgatgcc gttggcatta gtggcgatga catggaagca ttggctttcg
    15361 cctggcttgc ctggcggacg ctggcgggat taccaggaaa tctgccttcc gtcactggcg
    15421 caagccagga gacggtactg ggggctattt tccccgccaa ctcgtgatat cagaattaac
    15481 tgaatttttt cttatcgtgt ctgccgttac acttggaaga ttaaggaggg tatttgccct
    15541 ccggaccagg aaagtcttcg ggatatgact atgaaaaaac tgttaattat cattttgccg
    15601 gtccttctct ccggttgtag cgcctttaat caactggttg agcgaatgca aaccgatacg
    15661 ctggaatacc agtgtgatga aaaaccgttg acggtcaaac tgaataatcc gcgccaggaa
    15721 gtcagttttg tttacgataa tcaactactg catctcaaac agggcttttc agcctctggc
    15781 gcgcgttact ccgacgggat ctatgttttc tggtcgaaag gtgaggaagc gactgtctat
    15841 aaacgcgacc gcatcgtctt gaataactgt cagttacaaa atccacagcg ttgagatttt
    15901 tccaggggcg gcgcacaata gcgtcaccca ctgacaatcc gtaaagaaaa ccatgtctga
    15961 taacgacgaa ttgcagcaaa tcgcgcatct gcgccgtgaa tacaccaaag gcgggttacg
    16021 ccgccgcgat cttcccgccg atccattaac cctttttgaa cgttggctct ctcaggcttg
    16081 tgaagccaaa ctggcggacc ctaccgcgat ggtggtcgct accgtggatg aacatggtca
    16141 gccttatcag cgcatcgttt tactcaaaca ttacgacgaa aaaggcatgg tgttttacac
    16201 caacctcggt agccgtaaag cacatcaaat tgaaaataat ccgcgcgtta gcctgctgtt
    16261 cccgtggcat acccttgaac gccaggttat ggtgatcggt aaagcagaac gactttcgac
    16321 tctcgaagtg atgaaatatt ttcatagccg cccgcgtgat agccagattg gtgcatgggt
    16381 ttcgaagcag tccagtcgca tttctgcccg cggtatcctt gaaagtaaat tcctggagct
    16441 gaagcagaag tttcaacagg gcgaagtgcc attgccgagc ttttggggcg gttttcgcgt
    16501 cagccttgaa cagattgagt tctggcaggg cggtgagcat cgcctgcatg accgcttttt
    16561 gtaccagcgt gaaaatgatg cgtggaagat tgatcgtctt gcaccctgaa aagatgcaaa
    16621 aatcttgctt taatcgctgg tactcctgat tctggcactt tattctatgt ctctttcgca
    16681 tctggcgaaa agtcgtgtac cggcaaaggt gcagtcgtta tatacatgga gattttgatg
    16741 gcaagcagta acttgattaa acaattgcaa gagcgggggc tggtagccca ggtgacggac
    16801 gaggaagcgt tagcagagcg actggcgcaa ggcccgatcg cgctctattg cggcttcgat
    16861 cctaccgctg acagcttgca tttggggcat cttgttccat tgttatgcct gaaacgcttc
    16921 cagcaggcgg gccacaagcc ggttgcgctg gtaggcggcg cgacgggtct gattggcgac
    16981 ccgagcttca aagctgccga gcgtaagctg aacaccgaag aaactgttca ggagtgggtg
    17041 gacaaaatcc gtaagcaggt tgccccgttc ctcgatttcg actgtggaga aaactctgct
    17101 atcgcggcga acaactatga ctggttcggc aatatgaatg tgctgacctt cctgcgcgat
    17161 attggcaaac acttctccgt taaccagatg atcaacaaag aagcggttaa gcagcgtctc
    17221 aaccgtgaag atcaggggat ttcgttcact gagttttcct acaacctgtt gcagggttat
    17281 gacttcgcct gtctgaacaa acagtacgat gtggtgctgc aaattggtgg ttctgaccag
    17341 tggggtaaca tcacttctgg tatcgacctg acccgtcgtc tgcatcagaa tcaggtgttt
    17401 ggcctgaccg ttccgctgat caccaaagca gacggcacca aatttggtaa aactgaaggc
    17461 ggcgcagtct ggttggatcc gaagaaaacc agcccgtaca aattctacca gttctggatc
    17521 aacactgcgg atgccgacgt ttaccgcttc ctgaagttct tcacctttat gagcattgaa
    17581 gagatcaacg ccctagaaga agaagataaa aacagcggta aagcaccgcg cgcccagtat
    17641 gtactggcgg agcaggtgac tcgtctggtt cacggtgaag aaggtttaca ggcggcaaaa
    17701 cgtattaccg aatgcctgtt cagcggttct ttgagtgcgc tgagtgaagc ggacttcgaa
    17761 cagctggcgc aggacggcgt accgatggtt gagatggaaa agggcgcaga cctgatgcag
    17821 gcgctggtcg attctgaact gcaaccttcc cgtggtcagg cgcgtaaaac tatcgcctcc
    17881 aatgccatca ccattaacgg tgaaaaacag tccgatcctg aatacttctt taaagaagaa
    17941 gatcgtctgt ttggtcgttt taccttactg cgtcgcggca aaaagaatta ctgtctgatt
    18001 tgctggaaat aatgcattaa gtggaaaggg ggagtgagaa atcactcccc ctggttttta
    18061 tacagggaac atgatgaaaa atattctcgc tatccagtct cacgttgttt atggtcatgc
    18121 gggtaacagt gcggcagagt ttccgatgcg ccgcctgggc gcgaacgtct ggccgctgaa
    18181 caccgttcaa ttttctaatc acacccaata cggcaaatgg actggctgcg tgatgccgcc
    18241 cagccattta accgaaattg tgcaaggcat tgccattgcc gccattgata aattacacac
    18301 ctgtgatgcc gtattaagtg gctatctggg atcggcggag cagggtgaac atatcctcgg
    18361 tatcgtccgt caggtgaaag cagcgaatcc gcaggcgaaa tatttttgcg atccggtaat
    18421 gggtcatccg gaaaaaggct gtatcgttgc accgggtgtc gcagagtttc atgtacggca
    18481 cggtttgcct gccagcgata tcattgcgcc gaatctggtt gagctggaaa tactctgtga
    18541 gcatgcggta aataacgtcg aagaagcggt tctggcagcg cgcgaactca ttgagcaagg
    18601 gccacaaatt gtgttggtta aacacctggc gcgagctggc tacagccgtg accgttttga
    18661 aatgctgctg gtcaccgccg atgaagcctg gcatatcagc cgtccactgg tggattttgg
    18721 tatgcgccag ccggtaggtg ttggtgatgt gacgagcggt ttactgctgg tgaaactgct
    18781 tcagggggca acgctgcagg aggccctgga acatgtgacc gctgcagtct acgaaatcat
    18841 ggtgaccacc aaagcaatgc aggaatatga gctgcaagtg gtggctgctc aggatcgtat
    18901 tgccaaacca gaacattact tcagcgcaac aaagctctga aatatctcat ttcggcccac
    18961 aacgacctgt gggccgatgg ctattacttt aagccttccg ctgacagcgc gtcttgtact
    19021 tccggacgtt cagccatacg ttgcataaat gccgcaatgt gctctaaccc ttccagattc
    19081 agtttcaccg cgtatgccca gcgcagaacc gtaaacagat aggcatcagc aattgtaaat
    19141 ctttgcccgc agatccagtg ctcatccttc agtgcctcgt tcacatattg cagcttctta
    19201 tccagctgcg cgcgaactgt cggtttgtac tcttccggtg tatctgggcg aaacagaggt
    19261 gtgaaacctt tatgcagctc ggtggcgatg taattcagcc attcgatggt tttatagcgg
    19321 gaaatactgt ttaccggtgc cagcaactgg cggtcgggga cgctgtcggc aagatactgc
    19381 ataatcgcaa cgccttccgt cagcaaagta ccatcgtcca gcagcaatgc aggcacctgc
    19441 cccttagggt taacggaaaa gtaatcgtca ccgttttcga gacgtttttt cattaaatcc
    19501 acactgacga gcgtaaaatc ctttccgctc tcacgcaggg tgatatggga agcgagagag
    19561 caggcgcccg gtttgtagaa caatttcatc ggtaactcct tttgtctgtg gtttaaagta
    19621 tggtagtgcg ttgctgaaca aaaaaaaagc cgctaacgaa aagttagcgg cttgtaaaag
    19681 agtttccctg aattttacgc tacagtagct ttcgccgctt tgtctgcagc gtcatcctgc
    19741 gtcatgcggt gcagtttagg cgcggtcagc agcatcagta ctgcaataac ggcagtagcg
    19801 acaccaatct gcaagaatac gcgaccatag acttccagtg acatcagcgg gtcggtaacg
    19861 ttatccggca cagccatcat acccgcaaca taaccaccaa tcaggtttgc accggcagtg
    19921 gtcaggaacc agctacccat aatgaagccc atcagacgct gcggaacgag ttgtgcaacc
    19981 attgccagac ccagaccaga gatcatcagt tccccgatgc tctgcaggcc atagcttgcg
    20041 accagccagc tgacagacac gatacctgcg tcagacgcga atttcgcacc cagcggcaga
    20101 atcaggaacg caccagaaca catcaccatg ccgattgcaa acttggttgg catcggcagg
    20161 gtatcaccca tcttgttata gatagcggcc agaatcggac taccgatgat gatccagaac
    20221 gggttcagtg cctgatactg ctcaggttct acggccagac ccagaatgga gtgctcaacg
    20281 ttacgaatcg caaagaagtt cagtgacgtt ggcatctggc tgtacagcac gaagaagatg
    20341 attgcttcga gcatcaggat gaaggcaacg atcattttac gacgcgcagc acctttcatc
    20401 gcgaaagctt ctttaccgaa gataaccaca ataccgaagg caacaacgcc cagcgccata
    20461 cgcgcaactt cctgattgtg cagtagccag gtggcaacag cgatcagcgc cacaacacca
    20521 ataatggtca gcaacaggtt acggtagttg actggttcaa agtctggttt tgaaccgtac
    20581 tgtttaaccc agcgttggca gaaggcgaag ttaacgatag tgatcagcag gcctacaacg
    20641 ctcaacgcaa acgcaacact ccagccgtat ttcgcggcca gccacggggt ggctatcata
    20701 gagaagaaag agccgatgtt gacggacatg tagtacatgg tgaatgcacc gtccagacgt
    20761 gggtcgtttt tctcatagca tgtagaaagc agagaagacg ggttagcttt aaacaggccg
    20821 ttaccgaccg caatagccgc catacccata taaacaatac cggcgtcgtg accagaccag
    20881 gcaaccagag cataaccaat cgccagcaca atagcgccga gcataattac gcgtttagta
    20941 cccagtacct tgtcacctaa ccagccgccg atagcgacca gaccataaac cagggcacta
    21001 aaggaagaga aaagggtgat tgaatccgct tcagacatac ccagttgttt aaccaggtaa
    21061 acagccataa ttccttgtag gccgtaataa ccaaaacgtt cccataactc aatcgagaag
    21121 atgagataga acgccttcgg ttgtttgaaa gcgttcaaac tgacgctttc agttggtttt
    21181 tggtttgcag tggacacttt tacctctttt tttacatccc atattaacgg gggggctttc
    21241 tcgcgttatg tctaaaaaac atacgcgcaa ttgttatagt gggaggggaa acggcaggta
    21301 atgttcacta tcttgcactt tcagacaagt cctttgtaat aatctgttac atataactga
    21361 ggcggggaat tcaaccattc cgggaaatgt tatccagcgt taaatttttc aaaaaacgta
    21421 acggcagatt ttttcactgc attgagaggg ggatgatggc ttgacggaga tatgttctgc
    21481 tatttagcgt ttttcctagt gagatagtcc agtttctgaa aaatagccag tgtaatgttt
    21541 tgcaggtcaa tatttgttgc gtgtgtaaat ttaaatcatt ttaacctgat tcttgcaatg
    21601 tttttacatt gccacagcat cgtgatcttg atcacgaatg aatagaaaat ttcgctatta
    21661 aaaaaacaat taacctgcat ttttggttat ccgcaaacgg attacctgat gataacgcta
    21721 agcaatggca tcattgacgt taaaaagatg ttttaaaaga ggataaagaa aggttatcaa
    21781 tggggtaatc ggtgttaccc cttttcttca gatgtcaact ttctctttgt attcacaaag
    21841 atcttcaata atacaagagc cacagcgggg cttgcgggca atgcaggtat aacgcccgtg
    21901 caggatcaac caatggtggc agtcgacttt aaactctgcc ggaaccactt tcagtagctt
    21961 ttcttctacc tgttcgacgt ttttccccgg cgcaaattga gtacgattac aaacgcggaa
    22021 aatgtgcgtg tcgacagcaa tagtcggcca gccgaatgca gtgtttaata cgacgttggc
    22081 tgttttacga cctacgccgg gcagggcttc aagcgcagca cgatcttccg gaacctcgcc
    22141 attatgttgt tccagtaata tacggcaggt tttgatgata ttttctgctt tgctgttata
    22201 aaggccaatc gttttgatat aggttttcac cccttcaacg cccagttcaa gcatcgctgc
    22261 aggcgtattc gccaccgggt agagtttcgc cgtcgcctta ttaacactga tatcggtcgc
    22321 ctgagcggaa agcagtacgg caatcagcaa ttcaaaaggc gaactgaaat taagctcggt
    22381 ggtgggatga ggattgttct cacgcaggcg agtgaggatc tccagacgtt ttgctttgtt
    22441 catcagacat tccctgtttc accgtttggc aatgcacgtt cggcagctgc ttcagcacgg
    22501 cgctttttca ttctttcatc aatcaggtat tttcctgcca gcatcagtcc caagccaata
    22561 aatgcacctg gtggcagcat cgccagcagg aaaggggagt cggtgtggaa aatctccacg
    22621 cgtaatactt ttgcccagct acctaacagc gcatctgcac cgtcaaacaa cgtgccattg
    22681 ccgataattt cgcgtagtga acccagcacg aacatggcgc aggttgcgcc cgtaccaatt
    22741 gaaaagccgt ccagtgccga aagcgccgga ccttttttgg cggcgaaggc ttcagcgcgg
    22801 cccacaacga tacagttagt gacaatcagc ggaataaaaa tccctaatga ttgatacagg
    22861 ccaaaggcgt aggcgttgat cagcatctgt acagcgctga ccaccgaggc gatgatcatc
    22921 acgtaaatgg gaatgcggat ctcggctggc gtccagtgac gcagcgtcga aatggtcagg
    22981 ttggtcagcg tcagtaccag cgtagtcgca agtcctaaac ccagagcgtt agtggcagtg
    23041 gacgtgaccg ccaatagcgg acaaaggccg agcagttgga ccagtgcaga gttgtttttc
    23101 cacaacccct gaacaataac gtctttaatt tcgctcacgc tttattctcc acaggcagga
    23161 agttgagaaa gttgtgccgg taacgtctga gcgtacaatc cggcgcgttt taccgcatta
    23221 accaccgcgc ggggagtaat cgtcgcgccg gtgaactggt cgaaatcacc accatctttc
    23281 ttcaccgccc agtgcgcatc atccgcacca ctgatttttt ttcccgcaaa atgggtgagc
    23341 cagtcagaaa ggcgcaattc gattttatcg ccaagccctg gcgtttcgtg gtgctctgtc
    23401 acgcgcgtgc caagtaccgt gccattaaaa tcggctccca ccagcagctg aatcgcaccg
    23461 gaatagccat ctggcgcggt tgcttccaga acggcggcta ccggtttgtc atcctgtttg
    23521 gcgatgtaaa cccgatgctc acctttacct aactctggcg cagttaccag atagcaactc
    23581 tgtgcgagcg cattgttata gcgttcggct ggcagcacct gaccaaataa cgccttttgt
    23641 tgcagactgg cctgttcagc aattgtcgtt ttggtcatct ggttgatggc cgcagttaac
    23701 cctgtagaac ccgctgcaaa tagtgccagt gtaatgccgt gttttcggat agttttcagc
    23761 atggtttacc ctttgcgatg gccgtagacg cgcggacgcg tgtagtaatc gatcagagga
    23821 accgtgatgt tcgccagcag gacggcaaaa gccacgccgt caggatagcc gccgaaactg
    23881 cggatcaacc agactaataa gcccgccagc gcgccgaaca tcagccgacc acgattggtc
    23941 gtagaagcag taactgggtc agtcaaaata aagaatgcgc cgagcatagt cgcaccagac
    24001 agcagatgaa tttgcggtgc tgccagtgtt tctggtgaga acaaccagcc caacgttgcg
    24061 cataacgcca gcgttactaa gaagctgagg ggaatatgcc agcgaatcgc tttctgccat
    24121 agcaaccaca cgccaccagc cagccaggcg agatttaccc attgccaacc agcgcccgcc
    24181 agaataccgc tgtagatcgg atattgcata atctgttcaa ccgaatgacc tgcgcggaca
    24241 gaggttttga atgtatccag cggtgtcgcc tgactaatgc catcaatacc taagcgtagc
    24301 gtattcatat caccaccact ggcggtatga ccgctgaaaa taacctgtat ggcgtcgata
    24361 aaaccaggga tgttgaccgc aatttcatgt ggcggtaacc agctggtcat ctgcaccggg
    24421 aaggagatca gtaagaccac ataaccaatc attgccggat taaacgggtt ttgccccaga
    24481 ccgccataca actgtttagc gatgatcacc gcaaacaccg tacccagtac gaccatccac
    24541 catggcgcta gggggggaat acttaccgcc agcaataagc ctgtcagcaa tgctgagtta
    24601 tctttcaacg ttgcggctac cgactgcttg cgtagtttga gtacgagagc ttcggctaac
    24661 agagcactga ccgacgccaa caggatctga acgagagtac cccaaccaaa aaaccacagt
    24721 tgcgctgcga ttcctggcac ggctgcgagc aacaccaaca gcataatgcg cgatgtctgg
    24781 cgctggttat gggtataagg ggagctagct attctgaata ccatttagtc ctcgtttaca
    24841 accttttgct gggcggcttt tttggcctga acgcgggcaa tagccgcggc aactgcggct
    24901 ttgcgcggat cgacctgttc ttctggttcc gcattagcct gttgctgttc cagcttgcgt
    24961 gctttggcgc gggcaatagc ggcttcgacg gcagctttgc gcggatcaac ctgttcttct
    25021 ggctccgcat tggcctgttg ctgttccagc ttgcgtgctt tggcgcgggc aatagcggct
    25081 tcgacggcgg ctttgcgcgg atcgacctgt tcttctggtt ccgcattagc ctgttgctgt
    25141 tccagcttgc gcgc
//
LOCUS       DADMEQ010000053        24416 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.053, whole genome shotgun sequence.
ACCESSION   DADMEQ010000053 DADMEQ010000000
VERSION     DADMEQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.053"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..488)
                     /locus_tag="JNZ05_002172"
     CDS             complement(<1..488)
                     /locus_tag="JNZ05_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="required for cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY8565402.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LL"
     gene            761..949
                     /locus_tag="JNZ05_002173"
     CDS             761..949
                     /locus_tag="JNZ05_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565403.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            961..1671
                     /gene="bcsQ"
                     /locus_tag="JNZ05_002174"
     CDS             961..1671
                     /gene="bcsQ"
                     /locus_tag="JNZ05_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HAY8565404.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAEEILTLA
                     NWCLLNYSGLKTPVGSAS"
     gene            1668..4245
                     /gene="bcsA"
                     /locus_tag="JNZ05_002175"
                     /pseudo
     CDS             1668..4245
                     /gene="bcsA"
                     /locus_tag="JNZ05_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            4256..6595
                     /gene="bcsB"
                     /locus_tag="JNZ05_002176"
     CDS             4256..6595
                     /gene="bcsB"
                     /locus_tag="JNZ05_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HAY8565405.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNLPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIREYGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6602..7708
                     /gene="bcsZ"
                     /locus_tag="JNZ05_002177"
     CDS             6602..7708
                     /gene="bcsZ"
                     /locus_tag="JNZ05_002177"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HAY8565406.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7767..7943
                     /locus_tag="JNZ05_002178"
                     /pseudo
     CDS             7767..7943
                     /locus_tag="JNZ05_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            8271..10019
                     /gene="bcsC"
                     /locus_tag="JNZ05_002179"
                     /pseudo
     CDS             8271..10019
                     /gene="bcsC"
                     /locus_tag="JNZ05_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10101..12089
                     /gene="hmsP"
                     /locus_tag="JNZ05_002180"
     CDS             10101..12089
                     /gene="hmsP"
                     /locus_tag="JNZ05_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HAY8565407.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12273..13559
                     /locus_tag="JNZ05_002181"
     CDS             12273..13559
                     /locus_tag="JNZ05_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAY8565408.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            13780..15276
                     /locus_tag="JNZ05_002182"
     CDS             13780..15276
                     /locus_tag="JNZ05_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY8565409.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(15372..16520)
                     /locus_tag="JNZ05_002183"
     CDS             complement(15372..16520)
                     /locus_tag="JNZ05_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY8565410.1"
                     /translation="MFKSDAARTRQRLSARKGRTLPPGRGKFPHSTTESFRNTFWLKK
                     IMEHCFNMVDQQTTTAQTNANFLQIRFTTMSKKIAVIGECMIELSEKDADVKRGFGGD
                     TLNTSVYIARQVDPAALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGL
                     YYIETDSTGERTFYYWRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTS
                     REKLLSLLRECRAKGGKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDA
                     LWGQQPVEDVIARTHNAGVKEVVVKRGAGSCLVSIAGEALVDVPAVKLPKEKVIDTTA
                     AGDSFSAGYLAVRLTGGSVENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16533..17300
                     /gene="pdeH"
                     /locus_tag="JNZ05_002184"
     CDS             16533..17300
                     /gene="pdeH"
                     /locus_tag="JNZ05_002184"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HAY8565411.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            17370..19421
                     /locus_tag="JNZ05_002185"
     CDS             17370..19421
                     /locus_tag="JNZ05_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY8565412.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWSFRL
                     DNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFGLKA
                     QGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGGVDL
                     RLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGDSDI
                     HGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKSVQP
                     AGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQPLKF
                     GMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAELRG
                     SGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNCAAT
                     NLHIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSPLYV
                     RGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILSQMK
                     K"
     gene            complement(19603..20925)
                     /locus_tag="JNZ05_002186"
     CDS             complement(19603..20925)
                     /locus_tag="JNZ05_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAY8565413.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLAGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21336..22349)
                     /gene="yhjD"
                     /locus_tag="JNZ05_002187"
     CDS             complement(21336..22349)
                     /gene="yhjD"
                     /locus_tag="JNZ05_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HAY8565414.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22398..23369)
                     /locus_tag="JNZ05_002188"
     CDS             complement(22398..23369)
                     /locus_tag="JNZ05_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8565415.1"
                     /translation="MLEKTINNAICALLFRCEQQSVKEMDKIHAMQLFIKVAELESFS
                     RAADFFALPKGSVSRQIQALEHQLGTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSE
                     LDGLFQQDATSISGKLRIDIPSGIAKNLLLPRLSEFLYQHPGIELELSSHNRPVDILH
                     DGFDCVIRTGALPEDGVIARPLGKLTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTP
                     HLGVHPLGFEVASVNGVQWFKSGGMLTVNSSENYLAAGLAGLGIIQIPRIAVREALRA
                     GRLIEVLPGYRAEPLSLSLVYPQRRELSRRVNLFMQWLAGVMKEHLD"
     gene            23817..>24416
                     /locus_tag="JNZ05_002189"
     CDS             23817..>24416
                     /locus_tag="JNZ05_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY8565416.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5787 a         6151 c         6710 g         5768 t
ORIGIN      
        1 tcaagcaaca atgaaaattg tttatcgtta ttatttccgg gattaattac caaaagcgaa
       61 caatggttta acctgctcca tttattcatt ttatccaacc atgagcgaag ccgctcggca
      121 ggaatgtttt gccatgcgtt atttgcgcaa acaagaataa aaaggtaatt atggggatca
      181 atagaacact gcaaatcacg ggccaaatag tatagacctt tttcatgatt tagcattgaa
      241 aataatttta ttttttccgg cccttgagaa tcatctaatt gaaagatttt cgccggatcg
      301 ctgtccatgc taatgaccgc gacgtgtgcg gtttcagcct gggatgcaat tgtttgattc
      361 gccagactga tagcatcttc atggcgatcg acgttaaacc accagacgcc gcctgctggc
      421 atatgtcgca gctcatccca taatgatgag ataccgatag agaatacagg gtccacaatg
      481 tccctcatgt caccattttg tctgattatc agtttactag cgaaagctaa gaaataaacc
      541 taccattgaa attaaaactt atcagattta gcatgtgaat caatttagaa ggtcacaaga
      601 cgtgtgattt tagtcttttc gtttttaact ttttctgttg cgtaacggaa agtcaaaaag
      661 tgagcaaatt cccgtctcgc cgtaaacaat aaaccggcga aatgctcacg gttagaattg
      721 tatcatgaat ggtacggtta tttcataggg atcaagcaaa atgaataaca atgaaccaga
      781 tactctgcct gatcccgcga taggctatat cttccagaat gatattgtgg cgttaaagca
      841 ggcattttca ctgcctgata ttgattatgc cgatatttcc caacgcgaac agttggccgc
      901 ggcattaaaa cgctggccgt tgctggcaga gtttgcgcaa caaaagtagg ggattggtga
      961 atggccgtac tgggattgca gggggtgcgg ggaggcgtgg ggacaacaac catcaccgcc
     1021 gcattagcct ggtcattaca aatgttggga gaaaatgtcc tagtggtcga tgcctgcccg
     1081 gacaacttgt tgcgcctgtc atttaatgtt gattttaccc accgtcaggg ctgggccaga
     1141 gcgatgctgg atggccaaga ctggcgtgac gctgggttgc gctacacctc gcagctcgat
     1201 ttgctgcctt ttggtcagtt atccattgaa gaacaagaaa atccacagca ctggcaaacc
     1261 cggctgagcg atatttgctc cggcttacag caactaaaag ccagcgggcg ttaccagtgg
     1321 attttaatcg acttaccgcg tgatgcctcg cagataaccc accagctgct gagtttgtgc
     1381 gagcactcgc tggcaatcgt caatgtggat gccaactgcc atatccgact gcatcagcaa
     1441 gcgctgccgg atggcgcaca tattttgatt aatgacttcc gtattggcag tcaggttcag
     1501 gacgatattt accagctttg gttgcaaagc cagcgccgat tactgccgat gctcattcat
     1561 cgtgatgaag cgatggctga atgcctggcg gctgaagaga tactgacgct ggcgaactgg
     1621 tgcctgttga actactccgg gctgaaaacg ccagtcggga gtgcatcatg agtatcctga
     1681 cccggtggtt gcttatcccg ccggtcaacg cgcggcttat cgggcgttat cgcgattatc
     1741 gtcgtcacgg tgcgtcggct ttcagcgcga cgctcggctg tttctggatg atcctggcct
     1801 ggatttttat tccgctggag cacccgcgct ggcagcgtat tcgcgcagaa cataaaaacc
     1861 tgtatacgcc aacgcctcgc gtccgcgtcc gctggacccg gtccgttatc tcattcaaac
     1921 atgctggtta ttgatcggtg catcgcgcaa agaaacgccg aaaccgcgca gcgggcattt
     1981 tcaggtctgc aaaatattcg tggacgttac catcaatgga tgaacgagct gcctgagcgc
     2041 gttagccata aaacacagca tctggatgaa aaaaaagagc tcggtcattt gagtgccggg
     2101 gcgcggcggt tgatcctcgg tatcatcgtc accttctcgc tgattctggc gttaatctgc
     2161 gttactcagc cgtttaaccc gctggcgcag tttatcttcc tgatgctgct gtggggggta
     2221 gcgctgatgt tgattgtgct gtcgctgacc gtttcttgcc gttatatctg gtggcgttac
     2281 acctctacgc tgaactggga cgatccggtc agcctggtgt gcgggcttat tctgctcttc
     2341 gctgaaacgt acgcgtggat tgtgctggtg ctcggctact tccaggtagt atggccgctg
     2401 aatcgtcagc cggtgccatt gccgaaggat atgtcgctgt ggccgtcagt ggatatcttt
     2461 gtcccgactt acaacgaaga tctcaatgtg gtgaaaaata ccatttacgc ctcgctgggt
     2521 atcgactggc cgaaagacaa actgaacatc tggatcctcg atgacggcgg cagggaagag
     2581 tttcgccagt ttgcgcaaaa cgtgggggtg aaatatatcg cccgcaccac tcatgaacat
     2641 gcgaaagcgg gcaacatcaa caatgcgctg aaatatgcca aaggcgagtt cgtgtcgatt
     2701 ttcgactgcg accacgtacc aacgcgatcg ttcctgcaaa tgaccatggg ctggttcctg
     2761 aaagagaaac agctggcgat gatgcagact ccacaccact tcttctcgcc ggacccgttt
     2821 gaacgcaacc tgggacgttt tcgtaaaacg ccgaacgaag gcacgctgtt ctatggtctg
     2881 gtgcaggatg gcaacgatat gtgggacgcc actttcttct gcggttcctg tgcggtgatt
     2941 cgtcgtaagc cgctggatga aattggcggc attgctgtcg aaaccgtgac tgaagatgcg
     3001 catacttctc tgcgtttgca ccgtcgtggc tatacctccg cgtatatgcg tattccgcag
     3061 gcggcggggc tggcgaccga aagtctgtcg gcgcatatcg gtcagcgtat tcgctgggcg
     3121 cgcgggatgg tacaaatctt ccgtctcgat aacccgctca ccggtaaagg actgaagttt
     3181 gctcagcggc tgtgctacgt caacgccatg ttccacttct tgtcgggcat tccacggctg
     3241 atcttcctga ctgcgccgct ggcgttcctg ctgcttcatg cctacatcat ctatgcgcca
     3301 gcgttgatga tcgccctatt cgtgctgccg catatgatcc atgccagcct gaccaactcg
     3361 aagatccagg gcaaatatcg ccactctttc tggagtgaaa tctacgaaac ggtgctggcg
     3421 tggtatatcg caccaccgac gctggtggcg ctgattaacc cgcacaaagg caaatttaac
     3481 gtcaccgcca aaggtggact ggtggaagaa gagtacgtcg actgggtgat ctcgcggccc
     3541 tacatcttcc ttgttctact caacctggtg ggcgttgcgg taggcatctg gcgctacttc
     3601 tatggcccgc caaccgagat gctcaccgtg gtcgtcagta tggtgtgggt gttctacaac
     3661 ctgattgttc ttagcggcgc agttgcggta tcggtagaaa gcaaacaggt acgccgatcg
     3721 caccgcgtgg agatgacgat gcccgcggca attgcccgcg aagatggtca cctcttctcg
     3781 tgtaccgttc aggatttctc cgacggtggt ttggggatca agatcaacgg tcaggcgcag
     3841 attctggaag ggcagaaagt gaatctgttg cttaaacgcg gtcagcagga atacgtcttc
     3901 ccgacccagg tggcgcgcgt gatgggtaat gaagttgggc tgaaattaat gccgctcacc
     3961 acccagcaac atatcgattt tgtgcagtgt acgtttgccc gtgcggatac atgggcgctc
     4021 tggcaggaca gctacccgga agataagccg ctggaaagtc tgctggatat tctgaagctc
     4081 ggcttccgtg gctaccgcca tctggcggag tttgcgcctt cttcggtgaa gggcatattc
     4141 cgtgtgctga cttctctggt ttcctgggtt gtatcgttta ttccgcgccg cccggagcgg
     4201 agcgaaacgg cacaaccatc ggatcaggct ttggctcaac aatgatgata acgcgatgaa
     4261 aagaaaacta ttctggattt gtgcagtggc tatggggatg agtgcgttcc cctctttcat
     4321 gacgcaggcg acgccagcaa cgcaaccact gatcaatgct gagccagctg tagccgccca
     4381 gacggaacaa aatccgcagg tggggcaagt gatgtcaggc gagcagggag ctgatgcccc
     4441 catcgtggcg cagaacggtc cttcgcgtga tgtgaagctg acctttgcgc aaatcgcgcc
     4501 gccgccgggc agcatggtgc tacgtggtat taacccgaac ggcagcattg agtttggtat
     4561 gcgcagcgat gaagtggtga cgaaggcgat gctcaacctc gaatacacgc catcgccatc
     4621 gttactgcct gtccagtcgc agttaaaggt ttatctcaat gatgagctga tgggcgtgct
     4681 gccagtgacc aaagaacagt tgggtaaaaa aacgctggcg caaatgccca ttaatccact
     4741 gtttattacc gacttcaacc gtgtgcggct ggagtttgtc ggccattatc aggacgtgtg
     4801 cgaaaacccg gccagcacca cgctttggct ggatgttggg cggagcagtg gactggatct
     4861 gacctatcag accctgaatg tgaagaatga cctgtcacac ttcccggtgc cattctttga
     4921 cccgcgcgat aaccgtacta acaccttgcc gatggtcttt gcgggtgcgc cggatgttgg
     4981 gctgcaacaa gcatctgcca ttgtcgcctc gtggtttggt tcgcgttctg gctggcgtgg
     5041 gcagaacctc ccggtgctct ataaccaatt gccggatcgc aatgccattg tctttgcaac
     5101 caacgacaaa cggccggact tcctgcgcga tcatccggcg gtaaaagccc cggtgattga
     5161 gatgattaac catccgcaga atccttacgt caaactgctg gtggtgtttg gtcgtgacga
     5221 caaagacctg ttgcaggcag cgaaaggtat cgctcagggt aacattctgt tccgtggtga
     5281 aagcgtggta gtgaatgaag tgaaaccgct gctaccgcgt aagccgtacg atgcgccgaa
     5341 ctgggtacgt accgatcgtc cggtcacatt tggcgaactg aaaacctatg aagaacagtt
     5401 acaatccagc ggccttgagc cagcagcgat taacgtttcg ctaaacctgc cgccggatct
     5461 ctacctgatg cgcagtaccg gcattgatat ggatattaat taccgctaca ccatgccgcc
     5521 ggtgaaagac agttcgcgga tggatatcag cctgaataac cagttcctgc aatccttcaa
     5581 cctgagcagc aaacaggagg cgaaccgtct gctgctgcgt attccggtat tacaaggttt
     5641 gctggatggc aaaacagatg tctctattcc ggcgctgaaa ctgggcgcga ccaaccagct
     5701 gcgcttcgac tttgagtata tgaacccgat gccaggcggt tcggtagata actgtattac
     5761 cttccagccg gtgcagaatc atgtggtgat tggtgacgat tccaccatcg acttctcgaa
     5821 gtattaccac ttcatcccga tgccggatct acgcgccttt gctaacgcgg gcttcccatt
     5881 cagccggatg gcggatctgt cgcaaaccat caccgtgatg ccgaaagcgc ctaacgaagc
     5941 acagatggaa acgttgctga atactgttgg ttttatcggc gcacagacgg gcttcccggc
     6001 gattaatctg acggtgaccg atgatggcag caccattcag ggcaaagatg ccgacatcat
     6061 gatcatcggt ggtatcccgg acaaactgaa agacgataag cagatcgacc tattggtgca
     6121 ggcgaccgaa agctgggtga aaacaccgat gcgccagacc ccgttccccg gcattgtgcc
     6181 ggacgagagc gatcgcgcgg cagaaaccca gtcaacgctg acctcttccg gtgcgatggc
     6241 ggcggtgatt ggcttccagt cgccgtataa cgaccagcgc agcgtgattg cgctgttggc
     6301 agatagccca cgcggttatg aaatgcttaa cgatgcggtg aacgatagcg gcaaacgcgc
     6361 caccatgttc ggttcggtcg cggtgatccg cgagtacggt atcaacagcc tacgtgttgg
     6421 cgacgtttat tacgtaggtc atctgccgtg gttcgagcgc ttgtggtatg cgctggcaaa
     6481 ccatccgatt ctgctggcgg tgctggcggc tatcagtgtg atattgctgg catgggtact
     6541 gtggcgtctg ctgcgaatta ttagtcgtcg tcgtcttaac ccggataacg agtaattgaa
     6601 gatgaatgtg ttgcgtagtg gaatcgtgac gatgctgttg ttggctgcct ttagtgttca
     6661 ggcagcctgt acctggcctg cctgggagca gtttaaaaag gattacatca gtcaggaagg
     6721 gcgcgtcatc gaccccagcg acgcgcgcaa aatcaccacc tccgaagggc aaagttacgg
     6781 tatgttcttt gccctggcgg ctaacgaccg tgcagctttc gataatattc tcgactggac
     6841 gcagaacaat ctcgctcagg gttctttaaa agaacgtttg cccgcctggc tgtggggcaa
     6901 gaaagagaac agtaagtggg aagtgctgga cagcaattcg gcctccgatg gtgatgtctg
     6961 gatggcctgg tcgttgctgg aggcggggcg tttgtggaaa gagcagcgtt ataccgacat
     7021 cggcagcgcg ttgctaaaac gtatcgcgcg ggaggaagtg gtgacggtgc ctgggctggg
     7081 ttccatgttg ttaccgggca aagtgggttt tgctgaggat aacagctggc gttttaaccc
     7141 cagctacatg ccgccgacgc tggcgcagta tttcacccgc tttggcgcgc cgtggaccac
     7201 gctgcgcgaa accaatcaac atttattgct ggaaaccgcc ccgaaaggct tttcgccaga
     7261 ctgggtgcgc tatgagaaag acaaaggctg gcagctaaaa gccgaaaaaa cattgatcag
     7321 cagctacgac gctatccgcg tttacatgtg ggtaggcatg atgcctgaca gcgatccgca
     7381 aaaagcgcgg atgctcaacc ggtttaaacc gatggcgaca ttcactgaga aaaacggtta
     7441 tccgccggaa aaagtggatg tggctacggg gaaagcgcag ggtaaaggac cggtcggttt
     7501 ttctgccgcc atgctgcctt ttttacaaaa ccgcgatgcg caggcagttc agcgccagcg
     7561 cgtggccgat aactttcccg gcagcgatac ctattacaac tatgtgctga ccctgtttgg
     7621 acaaggctgg gatcaacacc gtttccgctt ctcgacaaaa ggtgagttat tacctgactg
     7681 gggccaggaa tgcgcaaatt cacactaaac atattcacgc tttccctcgg tctggccgtc
     7741 atgccgatgg tcgaggcagc accaaccatc agcagaccct gcggatggac agcggcaaca
     7801 ctaatgccgt gcgcgggctg gcgaatattt accgccagca gtcgccagaa aaagctgaag
     7861 cgtttatcgc ctcgctctct gccagccagc ggcgcagcat tgatgatatc gaacgcagcc
     7921 tgcaaaacga ccgtctggca cagcaggcag aggtactgga aaaccagggc aaatgggcgc
     7981 aggcggcagc acttcagcgg caacgactgg cgctggaccc cggcagcgta tggattactt
     8041 accgactttc gcaggatctc tggcaggccg gacaacgcag ccaggccgat acgttaatgc
     8101 gcaatctggc gcagcagaag ccgaacaacc cggagcaggt ttacgcttac gggctgtacc
     8161 tctcaggtca caaccaggac agagcggcgc tggcgcatat caacagcctg ccgcgcgcgc
     8221 agtggaacag caatattcag gagctggtta atcgactgca aagcgatcag gtgctggaaa
     8281 ccgctaatcg cctgcgagaa agcggcaaag aggctgaagc ggaagcgatg ctgcgtcagc
     8341 aaccaccttc cacgcgtatt gacctcacgc tggctgactg ggcgtaacaa cgacgtgatt
     8401 acaccgccgc ccgcactgca tatcagaatg tcctgacgcg ggagccaact aacgccgatg
     8461 ccattcttgg tctgacggaa gtggatattg ctgccggtga caaagcggcg gcacgtagcc
     8521 agctggcgaa actgcctgcc accgataaca cctcgctgaa cactcagcgg cgcgtggcgc
     8581 tggcacaggc gcagcttgga gataccgcag cggcgcagca gacgtttaat aagttgatcc
     8641 cgcaggcaaa atctcagcca ccgtcgatgg aaagcgcgat ggtgctgcgc gacggtgcga
     8701 agtttgaagc gcaggcgggc gatccaaagc aggcgctgga aacctacaaa gacgccatgg
     8761 tcgcatccgg tgtgactacg acgcgtccgc aggataacga cacctttacc cgactgaccc
     8821 gtaacgacga gaaagatgac tggctgaaac gcggcgtgcg cagcgatgcg gcggacctct
     8881 atcgccagca ggatcttaac gtcacccttg agcacgatta ctggggttcg agcggcaccg
     8941 gtggttactc cgatctgaaa gcgcacacta ccatgttgca ggtggatgcg ccgtattctg
     9001 acgggcggat gttctttcgc agtgatttcg tcaatatgaa cgtcggcagt ttctccacta
     9061 atgccgatgg caaatgggat gacaactggg gcacctgtac attacaggac tgtagcggca
     9121 accgcagcca gtcggattcc ggtgccagcg tggcggtcgg ctggcgaaat gacgtttgga
     9181 gctgggatat cggtaccacg ccgatgggct tcaacgtggt ggatgtggtc ggcggcatca
     9241 gttacagcga tgatatcggg ccgctgggtt acaccgttaa cgcccaccgt cggcccatct
     9301 ccagttcttt gctggccttt ggtgggcaaa aagactcccc gagcaatacc gggaaaaaat
     9361 ggggtggcgt acgtgccgac ggtgtggggc taagtctgag ctacgataaa ggtgaagtaa
     9421 acggcgtctg ggcatcgctt agtggcgacc agttaaccgg taaaaatgtc gaagataact
     9481 ggcgcgtgcg ctggatgacg ggctattact ataaggtcat taaccagaac aatcgccgcg
     9541 tcacaatcgg cctgaacaac atgatctggc attacgacaa agatctgagt ggctactcac
     9601 tcggtcaggg cggttactac agtccgcagg aatacctgtc gtttgccata ccggtgatgt
     9661 ggcgggagcg cacggaaaac tggtcgtggg agctgggggc gtctggctcg tggtcgcatt
     9721 cacgcaccaa aaccatgccg cgttatccgc tgatgaatct gatcccgacc gactggcagg
     9781 aagaagctgc gcggcaatcc aacgatggcg gcagcagtca gggcttcggc tacacggcgc
     9841 gggcattact tgaacgacgt gttacttcca actggtttgt tggcacggca attgatatcc
     9901 agcaggcgaa agattacgca cccagccatt tcctgctcta cgtacgttat tccgccgccg
     9961 gatggcaggg tgacatggat ttaccgccgc agccgctgat accttacgcc gactggtaag
    10021 ttttcagata gcgcctctct taatgccgct gcgatcgggt atactcgggc ggcaatctgg
    10081 gatttccggg gggagacaat ttgcgcgtaa gtcgctcgtt aacaatcaag cagatggcaa
    10141 tggtggcagc cgttgtcctg gtgttcgttt ttattttttg caccgttttg ctgttccatc
    10201 tggtccagca gaatcgctat aacacggcta cgcaactgga aagcattgct cgctctgtcc
    10261 gcgaaccctt atcttcagct attttgaaag gcgatattcc cgaagcggaa gctattcttg
    10321 ccagcattaa accggcaggc gtggtcagcc gtgccgatgt agtgctgcct aaccagttcc
    10381 aggcgctgcg taaaagtttt attccagagc gtccggtgcc ggtaatggtt actcgcctgt
    10441 ttgagctacc ggttcaaatc tcgctgggcg tctactcgct cgaacgtccg gcaaatccgc
    10501 agccaatagc ctatctggtg ctacaggcgg attccttccg tatgtataag ttcgtgatga
    10561 gcactctctc aacgttagtg accatttact tacttttgtc gcttatcctg accgtcgcca
    10621 tcagctggtg cattaaccgc ctgattttgc atccgttacg caatattgct cgcgaactta
    10681 acgccatccc agcccaggag cttgttggtc accaactggc attaccgcgt ctgcatcagg
    10741 acgatgaaat cggtatgttg gtgcgcagtt acaacctcaa ccagcaattg ctgcagcgcc
    10801 attatgaaga acagaacgaa aatgcgatgc gcttcccggt gtcggatttg ccgaacaaag
    10861 ccttgctgat ggagatgctg gagcaggttg tcgcgcgtaa acaaaccacc gcgctgatga
    10921 tcatcacctg tgaaaccctg cgtgatactg cgggcgtgct gaaagaggcg caacgagaaa
    10981 ttctgctgct gacgctggtg gaaaaactca aatcggtact gtcgccacgt atgatcctcg
    11041 cgcagattag cggttatgac tttgctgtca ttgccaacgg tgtacaggaa ccgtggcacg
    11101 caatcacttt gggtcagcaa gtactcacta tcatgagcga gcgcctgccg attgaacgta
    11161 ttcaactccg tccgcactgt agcattggcg tggcgatgtt ctacggcgat ctcaccgccg
    11221 aacagcttta cagtcgcgct atttctgcgg catttaccgc tcgccataaa ggcaagaatc
    11281 agattcagtt ctttgatccg cagcagatgg aagcagccca gaagcggttg acggaagaga
    11341 gcgatatcct taatgcactg gaaaatcatc agtttgcaat ttggttacag ccacaggtcg
    11401 agatgaccag cggtaaactg gtcagtgcgg aagtgttact gcgtatccag caaccggatg
    11461 gcagttggga cctgccggat ggcttaatcg atcgcattga gtgctgtggg ctgatggtta
    11521 ccgtcggtca ctgggtgctg gaagagtcct gtcgattgct tgcagcctgg caagagcgcg
    11581 gcattatgct gcccttgtcg gtaaacctct ctgcgctgca actgatgcac ccgaatatgg
    11641 tggcggatat gctggaactg ttaacccgct atcgcattca gccgggaaca ctgattctgg
    11701 aagtgacaga aagccgacgt attgacgacc ctcatgctgc ggtggcaatc ctccgtccgc
    11761 tgcgcaatgc cggagttcgg gtggcgctgg atgatttcgg tatgggctac gcagggctgc
    11821 gtcagctgca gcatatgaaa tcgttgccaa tcgacgtact gaaaatcgac aaaatgtttg
    11881 ttgaaggctt gccggaagat agcagcatga ttgctgcaat tatcatgctg gcgcagagcc
    11941 tgaacttaca aatgattgcc gaaggcgtgg agactgaagc acaacgagac tggctggcaa
    12001 aagcgggcgt tggtattgcc cagggcttcc tttttgctcg cccactccct attgaaatct
    12061 tcgaagagag ttacctggaa gaaaagtagc taccccaaac tgattacaaa actttaaaaa
    12121 gtgctggttt gtgcgagcca gctcaaactt tttaaccttt ttgtttcaat tatgatccag
    12181 gtacatttct gtgatgttgt ctgggtgtta ttttaaggcc gcaggtaccc ccataacctt
    12241 acaagacctg tggttttact aaaggacacc ctatgaaaac ctctctgttt aaaagccttt
    12301 actttcaggt cctgacagcg atagccattg gtattctcct tggccatttc tatcctgaaa
    12361 taggcgagca aatgaaaccg cttggcgacg gcttcgttaa gctcattaag atgatcatcg
    12421 ctcctgtcat cttttgtacc gtcgtaacgg gcattgcggg catggaaagc atgaaggcgg
    12481 tcggtcgtac cggcgcagtc gcactgcttt actttgaaat tgtcagtacc atcgcgctga
    12541 ttattggtct tatcatcgtt aacgtcgtgc agcctggtgc cggaatgaac gtcgatccgg
    12601 caacgcttga tgcgaaagcg gtagcggttt acgccgatca ggcgaaagac cagggcattg
    12661 tcgccttcat tatggatgtc atcccggcga gcgtcattgg cgcatttgcc agcggtaaca
    12721 ttctgcaggt gctgctgttt gccgtactgt ttggttttgc gctccaccgt ctgggcagca
    12781 aaggccaact gatttttaac gtgatcgaaa gtttctcgca ggtcatcttc ggcatcatca
    12841 atatgatcat gcgtctggca cctattggtg cgttcggggc aatggcgttt accatcggta
    12901 aatacggcgt cggcacactg gtgcaactgg ggcagctgat tatctgtttc tacattacct
    12961 gtatcctgtt tgtggtgctg gtattgggtt caatcgctaa agcgactggt ttcagtatct
    13021 tcaaatttat ccgctacatc cgtgaagaac tgctgattgt actggggact tcatcttccg
    13081 agtcggcgct gccgcgtatg ctcgacaaga tggagaaact cggctgccgt aaatcggtgg
    13141 tggggctggt catcccgaca ggctactcgt ttaaccttga tggcacatcg atatacctga
    13201 caatggcggc ggtgtttatc gcccaggcca ctaacagtca gatggatatc gtccaccaaa
    13261 tcacgctgtt aatcgtgttg ctgctttctt ctaaaggggc ggcaggggta acgggtagtg
    13321 gctttatcgt gctggcggcg acgctttctg cggtgggcca tttgccggta gcgggtctgg
    13381 cgctgatcct cggtatcgac cgctttatgt cagaagctcg tgcgctaact aacctggtcg
    13441 gtaacggcgt agcgaccatt gtcgttgcta agtgggtgaa agaactggac cacaaaaaac
    13501 tggacgatgt gctgaataat cgtgcgccgg atggcaaaac gcacgaatta tcctcttaat
    13561 cccaccactt atgcccgcac tcccttcccc gagtgcgggt gtttgcgcat aattgacccc
    13621 tgtgcctgaa cttaccacct gggtgagttc aggtttattt cacttcttcc gtgacatttt
    13681 catgttcttg cggtctaaca cgaagtgttt ttaacgtcat ttagactgac cgacaacgga
    13741 cagttttttt attaccagga tagttgatca ggggttcaca tgcagggcat aaaaattcga
    13801 cttttagcgg gcggtttgct gatgatggcc actgctggct atgtgcaggc agatgcgctc
    13861 cagcctgatc cagcatggca acaggggacg ctttccaacg gtttacagtg gcaagtgctg
    13921 accacccccc agcgtcccag cgatcgtgtt gaaattcgcc tgctggttaa taccggttcg
    13981 ctcgccgaaa gtacacaaca gagcggttac agtcacgcca tccctcgtat tgcgctaacg
    14041 caaagcggtg gccttgacgc agcacaggcg cgttcattgt ggcagcaggg gatcgaccct
    14101 aaacgcccga tgccgccggt aattgtctct tatgacacca cgctgtttaa tctgagtttg
    14161 cccaataacc gtaacgattt gctgaaagaa gcgctctctt atctggcaaa tgccactggc
    14221 aaattgacca tcacaccaga aaccatcaac cacgcgctgc aaagtcagga catggtggca
    14281 acctggcctg ccgatactaa agagggctgg tggcgctatc gtctgaaagg gtcaaccttg
    14341 ttaggtcacg atcctgccga tccgctgaaa caacccgttg aagcggaaaa aattaaagat
    14401 ttctatcaga aatggtacac cccggatgca atgacgctac tggtggtggg aaacgtggat
    14461 gcgcgctcgg ttgtcgacca aatcaacaaa acgtttggcg aactgaaagg caaacgtgaa
    14521 acgccagctc cggtgccgac gctttctccg ctgcgtgcgg aagcggtgag tattatgact
    14581 gacgcggtgc gtcaggaccg gttatctatc atgtgggata cgccgtggca gccgattcgt
    14641 gaatcagccg cactgctgcg ctactggcgt gcggacctgg cccgcgaggc gctgttctgg
    14701 catgttcagc aagcgttaag cgccagtgac agcaaagaca tcggtcttgg atttgactgc
    14761 cgtgtgctgt atctgcgtgc gcagtgtgcc atcaacatcg aatcaccaaa cgacaagctg
    14821 aacagcaacc ttaatctggt ggcgcgtgaa ctggcgaagg ttcgcgataa aggtctgccg
    14881 gaagaagagt tcaatgcgtt agtggcgcaa aagaaactgg agctgcagaa actgtttgcc
    14941 gcctatgcac gggctgatac cgatattctg atgggccagc ggatgcgttc gttgcaaaat
    15001 caggtcgtcg atatcgcgcc ggagcagtat cagaaactgc ggcaggattt ccttaacagc
    15061 ctgactgtgg agatgttaaa tcaggatctg cgtcagcagt tgtcgaatga tatggcgtta
    15121 atactgctgc agccgaaagg cgagccggaa tttaacatga aagcgttgca ggcggcctgg
    15181 gatcaaatca tggccccatc taccgccgct gcgaccacct ctgtcgccac ggatgacgta
    15241 catcctgaag tgacggatat tccacctgca cagtaatgcc tgatggtgat gagttagcac
    15301 aaccatcagg tatttatgga tgagctggat agaggggtaa cactttatcc agccttttgc
    15361 atatgctgcg tttacgctgg catcgcctca cgcgggataa tcgcgccgcg atactgaata
    15421 acggtacttg cggtcaggtg cccgcgtttc gccgcgtttt ccacactgcc gcccgtcaaa
    15481 cgtaccgcca gataaccggc gctgaaagag tcgccagctg cggtggtatc gatcactttt
    15541 tctttcggca gtttcaccgc cggaacatca actaacgctt caccagcaat ggacaccaag
    15601 caagaacccg ccccgcgttt caccaccact tctttcacgc ccgcgttatg ggtgcgcgca
    15661 atgacgtctt ccaccggctg ttgaccccac agcgcgtctt cgtcgtccag cgtcaggaag
    15721 gcgatatccg tgcattccag catttgctgg tacacctgct gtgtctcttc tttgctggcc
    15781 cacaggcgcg gacgatagtt attgtcgaaa atcacttttc cgcctttggc gcggcattcg
    15841 cgcagcaggg aaagcagctt ttcgcggctg gtcgggctta agatcgccag gctaatcccg
    15901 ctcaggtaga gataatcgaa attcgccagc tcttcgcaaa tcgccgcaga ctgctcgctc
    15961 gccagccaga atttggcggc ggcttcgtta cgccagtagt agaacgtacg ttcgccggtg
    16021 ctgtcggttt caatgtagta aaggcccgga agacggtttt ccatccgttg ggtcagggaa
    16081 gtatcaacgt tctcgccgtg ccaggcgtcc agcatctgct ggctaaaact gtccgttccc
    16141 agcgccgtta cgtaatgaac ggttaatgcc gcaggatcga cctgacgggc gatatagacg
    16201 gaagtgttca gggtatcgcc gccgaaaccg cgcttaacgt ccgcgtcttt ctcggaaagc
    16261 tcaatcatgc attcgccaat cacggcaatc tttttagaca tagtcgtgaa cctgatctgt
    16321 aaaaaattag cgttagtttg cgctgtggtg gtttgctggt caaccatatt aaaacagtgt
    16381 tccattattt ttttgagcca gaacgtattc ctgaaagatt ccgttgtgga gtgaggaaat
    16441 ttaccccggc caggcggtaa agttctgccc ttacgcgccg ataatctttg tcgagtccgg
    16501 gcagcatcac ttttaaacac aggacatctt tgatgataag gcaggttatc cagcgaataa
    16561 gcaaccctga agcaagcatc gagagcttgc aggaacggcg tttttggttg cagtgtgagc
    16621 gtgcttacac ctggcagccg atctatcaaa catgcgggcg gttaatggcc gtggagctat
    16681 taacggtggt cacgcatccc ttgaaccctt cgcaacgcct gccgccggat cgctatttta
    16741 ctgaaatcac cgtcagccat cggatggagg ttgtgaaaga gcagattgat ttgctggcgc
    16801 aaaaagccga cttctttgta gagcacggcc tgctggcatc ggtcaatatt gatggcccta
    16861 cgctcatcgc cctgcgtcag caaccaaaaa tcctgcgcca gattgagcgt cttccctggc
    16921 tgcgtttcga actggtggag catatccgtc tgccgaaaga ttcaaccttt gcctcgatgt
    16981 gtgaatttgg cccgctgtgg ctggatgatt ttggtaccgg gatggcaaat ttctctgcac
    17041 taagtgaagt gcgttatgac tacatcaaaa tcgcgcgaga actgtttgtg atgctgcgtc
    17101 agtcgccgga aggccgcaca ctcttttctc agcttttaca tctaatgaat cgctattgtc
    17161 gcggggtgat tgtcgagggc gtagaaacac cggaagagtg gcgtgatgtt cagaactcgc
    17221 ccgcattcgc cgcacaaggc tggtttcttt cacgcccggc accgatggaa acgctgaata
    17281 cggcggttct ggcgctataa gctgcctcat tttccgccgg gctggactat ctttaggact
    17341 ggcacaggaa gaatatgagg aagcgaacga tgagcaaggc aggcaaaata accgctgcga
    17401 tttcaggggc tttcttgttg ttgattgtcg tggcgatcat tttgattgca acttttgact
    17461 ggaatcgact caaaccgacc atcaaccaga aagtctctgc ggagttgaat cgtccgttcg
    17521 ctatccgtgg cgatctgggc gtggtgtggg agcggcaaaa acaagaaact ggctggcgca
    17581 gctgggtgcc gtggcctcat gtacatgcag aagacatcat tcttggtaat ccaccggata
    17641 ttcccgaagt cacgatggtg catttgccgc gagtagaggc accgctggcg ctgctgacca
    17701 aaacggtctg gctgccgtgg atcaagctcg aaaagcccga cgcgcgcctg attcgcctct
    17761 ctgaaaagaa caataactgg acgtttaatc ttgccaacga tgacaacaaa gacgcgaatg
    17821 caaagccgtc ggcatggtcg tttcggctgg ataatattct tttcgatcaa gggcggatcg
    17881 ccattgatga caaagtaagc aaagcggatc tggaaatttt tgttgatccg ttaggcaagc
    17941 cgctgccatt cagcgaagtt actggatcga aaggtaaagc ggataaagaa aaggtgggcg
    18001 attacatttt tggcctgaag gcgcagggac gttataacgg tgaaccgctc acgggtacgg
    18061 gaaaaatagg cggtatgctg gcgctgcgtg gcgaagggac gccgtttccg gtacaggctg
    18121 atttccgttc aggtaatacc cgtgttgctt ttgatggcgt cgtgaatgac ccaatgaaga
    18181 tgggcggtgt cgatttacgg cttaaatttt ctggcgattc actgggtgat ctctatgaac
    18241 tgacgggcgt tctgctgccc gataccccgc cgtttgaaac ggatggtcgg ctggtagcga
    18301 aaatcgacac tgaaaaatcg tcggtctttg attatcgcgg ttttaatggg cgaattggcg
    18361 atagcgatat ccacggttct ctggtctaca ccaccggaaa gccacgacca aaactggaag
    18421 gtgatgtcga gtcgcggcaa ttgcggctgg cggacctggg accgttgatt ggcgttgatt
    18481 ccgggaaagg ggcagaaaag tcgaaacggt ctgaacagaa gaagggcgaa aaaagcgttc
    18541 agcctgcggg caaagtgctg ccttatgacc gcttcgaaac cgataaatgg gacgttatgg
    18601 atgccgatgt tcgcttcaaa gggcggcgca ttgagcatgg cagtagcctg ccgattagcg
    18661 atctttctac tcatatcatc ctcaaaaatg ctgacctgcg cctgcaaccg ctgaaatttg
    18721 gcatggcggg cggcagcatt gcggcgaata ttcatctgga aggcgataaa aagccgatgc
    18781 aggggcgggc agatattcag gctcgtcgac tgaaactgaa agaactgatg cccgatgtgg
    18841 aactgatgca gaagacgctg ggggaaatga acggtgacgc ggaactacgc ggtagcggta
    18901 actcggtggc ggcgctttta ggcaacagta acggcaacct gaaactgttg atgaatgacg
    18961 ggctggtgag ccgcaacctg atggagattg ttgggctgaa tgtcggcaac tacattgtcg
    19021 gtgcgatatt tggtgacgat gaggtgcggg tgaactgcgc ggcgacgaat ctgcatattg
    19081 ccaacggcgt ggcgcgcccg cagatttttg ctttcgatac tgagaacgcg ttgattaatg
    19141 ttaccggcac ggcaagtttt gcttcggaac agctggattt gactattgat ccggagagta
    19201 aagggattcg gattatcaca ctgcgttcgc cgctgtatgt gcgtgggacg tttaaaaatc
    19261 cgcaggctgg ggtgaaagcc ggaccgctga ttgcccgtgg tgctgttgct gcggcactgg
    19321 caacgctggt aacaccggcg gcggcgttac tggcactgat ctcaccttcc gaaggggagg
    19381 ctaatcagtg tcggacgata ttgtcgcaga tgaagaagtg atgataattg ccggatgtct
    19441 gacatccggc aattaaacag atttagattt ttggcaaagt gtgctttgtt catgccggat
    19501 gcggcgtgaa cgccttatcc ggcctacaaa tccttgtgaa ttcaatatat tgtatggaaa
    19561 ttcagtcctg ataagcgtag cgcatcaggc ttttcacact tattacaacg actgatgtcg
    19621 cgtctcatgg gtcagcagca gggcgattaa cgtcaagcca gccatcgccg ccagatataa
    19681 ccccaccgca cctaacccgt agttagcctg caaccaagct gcgatatacg gcgcaacgga
    19741 agccccgaga atcgacgata cgttgtaaga gaacgatgct ccggtgtaac gcacttctgt
    19801 cggaaacagc tctggtaaca gcgcacccat cggcccgaag gtcagcccca tcagacttaa
    19861 ccccagcagc aggaaggcaa aaaccagaat cgggttgcca gaaccgagca gcgggttaaa
    19921 ggcgaacagc gcgaacagga tgatcagcgt ggtgatgatc accatgcttt tacggcgacc
    19981 aaaggcatca gccagtaatc cggcgactgg caccatcacg ccaaaaccaa taactgccat
    20041 catcagcatc cacaacactt cgttacgcgg caggccaagc ccaactggcg cggcggcggt
    20101 actgaaggtc atagagtaga ccgtcatgat gtaaaacagc gtataggttg ccagcataat
    20161 gaacgtaccc agtacggtta cgcgaacatg tttggtcagc agcgtaccca gcgggatctt
    20221 cacctgtttt ttcgctttag cgactttctc aaacaccggc gactcatgca gcgacacgcg
    20281 aacatacagg ccgataatga ccagcaccgc cgagaagata aatggcacac gccagcccca
    20341 gctcataaac tgctcatcgg tcagtagcca ggaaagcagc aagaaagtgc cgttggcaaa
    20401 gaagaagcca atcggtgcgc ctagctgtgg gaaggagcca tacagtgcac gtttgcgcgg
    20461 tggggcgttt tcagtcgcca gcagcgccgc gccgccccat tcaccgccta agcccagacc
    20521 ctgaccaaat cgagccagcg ccagcagcag tggggcgaaa ataccaatcg tggcatagcc
    20581 cgccagcaga ccaatcacca cggtcgaaat ccccatcgtt agcaacgagg cgaccagcgt
    20641 cgctttacgc ccaacgcgat cgccaaaatg accaaaaacg gcagagccaa tggggcgcgc
    20701 gacgaaggcg atggcgaagg tggcgagcga ctgtagcgtt gctgccgttg gatcgccctg
    20761 cgggaagaag atatgcggaa acacaataac ggccgcagtg gcgtaaatgt aaaagtcgaa
    20821 gaactcaatg gccgtgccaa tgagagaggc gacaaggact ttattacgcg agttgatcgg
    20881 cgtgtattct tgttcgtggt cgagtgttgt ggctgttgct tgcataatct tttcttattt
    20941 tttggctaac gaatagccat attacgcaca gcaaaagtga tatttcaatg tgtaacaaat
    21001 cgcggaaatg gtcaaaaacc aggcaaaaag gggataatgt ttaggccagt gatttttctt
    21061 ctatgaaatg cttcacataa ttttataaat agtggataaa actggtttca ggttaaataa
    21121 aagttaccaa ctggatttat atgctggggt tatgagttgg agtgaaattg tttgctccca
    21181 aatagatggc tgaagaggct ctggggaaga gtttcaagag gttatgtgcg cattatagcc
    21241 ggatgcgatg ctggcgcatc ttatccggcc tacggggagc caggccgaat aaaatcgtat
    21301 gccggataag gcgtttacgc cgcatccggc ctattttaag gctgcgtttt ccctggcatt
    21361 cgcgggtcgt ctttatattc ggcggtggca atccacgccg cgcaaaacag cgtcaaacgg
    21421 gcgaagaagt agaaaaatgc catcagcccc agcacggaac caaatgctgc gccagagggg
    21481 gatttcatca gcgatggcag ggtgtaggtc atcacgattt taatcacttc aaaaccaata
    21541 gcagccagaa atgttccgcg aatcagggct tttttgcgcg gacggtggcg cggcaaccgc
    21601 cagaagatcc agaagaaaag cagatagttg gcgaagatgg aaatcgccag gccaatcaat
    21661 cgccacgtcg gtttcagcca ctcaatgctg ttcaggtgca gggcgctaat aatcatttgc
    21721 tgcgccgaac cggcaaccga ggtgatcgaa agcgtcacaa tcagcgcaat caacaaacca
    21781 atcagtgaaa taaaatcacg cagatatttt acccagaact tctcctgatc ttgcggcgag
    21841 cgttcccaga catcgcgcga ctgggcacga atcgcttcac gcaggttacc catccagttg
    21901 atgccggaat aaagtgccac cgccaggccg acaagcccta cagtcgtacg ctgctgaacg
    21961 gcggtgttga tggtgttttt caacgtggcg gctagtgtcg gatcgctgat gttttgcaga
    22021 attttgtcga agatatcctg tagcaacatc ggatgggagg ccagcacaaa gccccctgcg
    22081 gcaaacgaca ccatcaaaat cgggatcatc gacaaaaatg agaaataggt gatagccgca
    22141 ccaaactggt tacccagccg atcgttaaag cgttctgtcg cgcgaataag gtgcgcaata
    22201 accggttggc gttggacttt ttcggcagtg gtggtgacgg tttccagcgc ctggctaact
    22261 ttgccgcctt tctcgctatt atccaactgc ttaatcggct cgtgctccag atcctgggtg
    22321 ggacgtttga tctcgttttc ctgcgtcatc cgctcttttg tccttctgtc gtggtcattc
    22381 ttaaaaagta tagtcggtca gtccaggtgc tctttcatta cgccagccag ccactgcata
    22441 aacaggttta cacgccggga aagctcccga cgctgcgggt aaaccagcga aagggagagc
    22501 ggctcggcac ggtagcctgg caacacttca ataagccgcc cggcacgcag ggcttcgcgc
    22561 acggcaatgc gcgggatctg aataatcccc agaccggcaa gaccggcggc gagatagttt
    22621 tcgctactgt ttaccgtcaa catgccgcca gacttaaacc actggacgcc attaacgctg
    22681 gcaacctcaa aacctaacgg atgtacaccc aggtgcggtg tataacgcac tattgcgtgt
    22741 gaagtcagat catcggggct ttgcggataa ccaaagcgcg tcagatagtg cggactggca
    22801 cagttgacca tggttagttt gccgagggga cgggcgataa cgccatcttc cggtaacgcg
    22861 ccagtgcgta tcacgcaatc aaaaccatca tgaagaatat ctaccggacg gttatggcta
    22921 ctcagttcca gctcaattcc cggatgctga tagagaaatt ccgacagacg cggcagtaac
    22981 aggtttttcg cgattcctga cgggatgtcg atgcgtaatt taccgctgat actggtggca
    23041 tcctgttgaa acagaccgtc cagttcgctg agattactca acacatcttt tgcccgttga
    23101 taataggtca tgccttctgg cgtgagtttg acccgtcgcg tagtgcgctg gagaagctgg
    23161 gtgccaagtt gatgttccag tgcctgtatc tggcgcgaaa cacttccctt tggcaaagca
    23221 aagaaatccg ccgcgcggga aaaactttcc agctccgcga ctttgatgaa caactgcatt
    23281 gcgtgaattt tatccatttc tttgaccgat tgttgttcac agcgaaacag taacgcgcat
    23341 atcgcattgt ttattgtttt ttctaacatc taatgccggg tttcacaatg ttgtcatgac
    23401 gaatgtggcg atttctcagc aggaaaaggg cggaatagct ttatgagaaa ggtgaatccc
    23461 cttaaacaga gcgtattttt cagtgattcc ctttaaagtg aatagaggac gtattggcta
    23521 tttgtgctta tttttgtctc attgccattt gttattttgg cttgtagtcg cggaatttca
    23581 tttttaattg catgcgtact aaccaccaat ataaggaaat cctcaaaatt gttttaaata
    23641 gtgccctcga catgtaattt caggcgttta gctgtaacta tttatatcgg taactatttt
    23701 ctacgcgtca tctctgtaga atggccaccg ccaaatggcg aattcttatt ttttctcgcg
    23761 ggtaataccc ttccactact taagtctttt taacgtattc aggtggagat tcagttatgc
    23821 aaatagtcat gtttgacagg cagtcaatat ttattcatgg aatgaaaatc agtttacagc
    23881 agcgtattcc aggagtgagt attcaggggg tcagtcaggc agacgagtta tggcaaaagc
    23941 tggaaagtta ccctgaagcc ttagttatgc tcgatggcga tcaggatggt gagttttgct
    24001 actggttgct gcaaaaaacc gtggtgcagt ttcctgaggt taaggtgtta atcacggcga
    24061 cggattgcaa taaacggtgg ttacaggaag ttatccattt taatgtgctg gccattgtgc
    24121 ctcgcgattc aaccgtcgag acatttgcgt tggcggtaaa tagtgcggcg atggggatga
    24181 tgtttctgcc tggagactgg cggacgacgc cggaaaagga cattaaagat ctcaaatcgt
    24241 taagcgcccg ccagcgggag attttaacca tgttagcagc gggcgaatca aataaggaga
    24301 tcggcagagc gctgaatatc agtaccggaa cggtaaaagc gcatctggaa tccctgtatc
    24361 gccgtctgga agtgaaaaat cgcacccagg cggcaatgat gttaaatatc tcctcc
//
LOCUS       DADMEQ010000054        24188 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.054, whole genome shotgun sequence.
ACCESSION   DADMEQ010000054 DADMEQ010000000
VERSION     DADMEQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.054"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(22..594)
                     /locus_tag="JNZ05_002190"
     CDS             complement(22..594)
                     /locus_tag="JNZ05_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565417.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="JNZ05_002191"
                     /pseudo
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="JNZ05_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="JNZ05_002192"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="JNZ05_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HAY8565418.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKLVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="JNZ05_002193"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="JNZ05_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAY8565419.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="JNZ05_002194"
     CDS             complement(3556..5337)
                     /locus_tag="JNZ05_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY8565420.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYNTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="JNZ05_002195"
     CDS             complement(5330..7102)
                     /locus_tag="JNZ05_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY8565421.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSELVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIHEEAVDA"
     gene            complement(7132..7590)
                     /locus_tag="JNZ05_002196"
                     /pseudo
     CDS             complement(7132..7590)
                     /locus_tag="JNZ05_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="JNZ05_002197"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="JNZ05_002197"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HAY8565422.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLSSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8662..10361
                     /locus_tag="JNZ05_002198"
                     /pseudo
     CDS             8662..10361
                     /locus_tag="JNZ05_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10426..11121
                     /gene="queC"
                     /locus_tag="JNZ05_002199"
     CDS             10426..11121
                     /gene="queC"
                     /locus_tag="JNZ05_002199"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HAY8565423.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11173..11571)
                     /locus_tag="JNZ05_002200"
                     /pseudo
     CDS             complement(11173..11571)
                     /locus_tag="JNZ05_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
     gene            complement(11665..12036)
                     /locus_tag="JNZ05_002201"
     CDS             complement(11665..12036)
                     /locus_tag="JNZ05_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565424.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12187..14058)
                     /gene="ppiD"
                     /locus_tag="JNZ05_002202"
     CDS             complement(12187..14058)
                     /gene="ppiD"
                     /locus_tag="JNZ05_002202"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY8565425.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNETESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14250..14522)
                     /gene="hupB"
                     /locus_tag="JNZ05_002203"
     CDS             complement(14250..14522)
                     /gene="hupB"
                     /locus_tag="JNZ05_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HAY8565426.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14731..17085)
                     /gene="lon"
                     /locus_tag="JNZ05_002204"
     CDS             complement(14731..17085)
                     /gene="lon"
                     /locus_tag="JNZ05_002204"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAY8565427.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17273..18547)
                     /gene="clpX"
                     /locus_tag="JNZ05_002205"
     CDS             complement(17273..18547)
                     /gene="clpX"
                     /locus_tag="JNZ05_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HAY8565428.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELDKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18673..19296)
                     /gene="clpP"
                     /locus_tag="JNZ05_002206"
     CDS             complement(18673..19296)
                     /gene="clpP"
                     /locus_tag="JNZ05_002206"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAY8565429.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19543..20841)
                     /gene="tig"
                     /locus_tag="JNZ05_002207"
     CDS             complement(19543..20841)
                     /gene="tig"
                     /locus_tag="JNZ05_002207"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAY8565430.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21185..21502)
                     /gene="bolA"
                     /locus_tag="JNZ05_002208"
     CDS             complement(21185..21502)
                     /gene="bolA"
                     /locus_tag="JNZ05_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HAY8565431.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21807..22385
                     /locus_tag="JNZ05_002209"
     CDS             21807..22385
                     /locus_tag="JNZ05_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY8565432.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22429..23904
                     /gene="ampG"
                     /locus_tag="JNZ05_002210"
     CDS             22429..23904
                     /gene="ampG"
                     /locus_tag="JNZ05_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HAY8565433.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5672 a         6657 c         5832 g         6027 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacggtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattg cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgctag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg aagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccaga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcactc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccgctgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcccacgagt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctca gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacgttgg cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcaa gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gttgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gagatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact acgcctactg ccgcccgatt aaacagcagc gactgagcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gtgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccagccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgagttcgct accatcgttc accaccggcg cttccgccag catcgcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cagcaatcag caccatggtg gcgatccaca tcaggatcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctagacgcgc atcagataat cgtattcacc agccatgcgc cagaacccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtcagcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ctgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctgc cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 ataatattgt tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg
     8701 tggcaacctt cagccggaaa gccgcaaacc gtcaccgtca gcgaactggc cgaacgctgt
     8761 ttttgcagcg aacgccatgt tcgtacgctg ttgcgtcagg cacaggaggc gggatggctg
     8821 gagtggcagg cgcagtcagg acgcggaaag cgcggacaat tacgctttct ggtcacgccg
     8881 gaatcgctac gcaatgcgat gatggaacag gcactggaaa ccggaaagca gcaagatgtg
     8941 ctggagctgg cgcaactggc cccaggtgag ctgcgcactc tgttacagcc gtttatgggc
     9001 ggacaatggc aaacgataca cccacgttgc gtattcccta ctatcgcccg ctcgaaccgc
     9061 tacaaccagg ctttttgccc ggccgtgccg agcagcatct cgccgggcag atattttccg
     9121 gcctgacccg cttcgataat aatactcagc gcccgattgg cgatttagcg catcactggg
     9181 aaacctctac tgacgggtta cgctgggact tttatcttcg ttcaacccta cactggcata
     9241 acggcgatgc agtaaaagcc tcacacttac accagcgatt attgatgctg ttacaactgc
     9301 cagcactgga tcaattattt attagcgtga agcgtattga agtcacccat ccgcagtgtc
     9361 tgaccttctt tttacatcgc cccgattact ggcttgcgca ccggctggcg agctattgca
     9421 gccatctggc gcatccgcaa ttcccactga tcggcactgg tccttttcgc ttaacacaat
     9481 tcacagcgga gctggtgcgc ctggagagcc atgattatta ccatttgcgt catccgctgc
     9541 ttaaagcggt tgagtactgg ataactccgc cgcttttcga aaaagatttg ggaaccagtt
     9601 gtcggcatcc cgtgcaaatc accatcggca aaccggagga gctgcaacgg gtcagccagg
     9661 tcagtagtgg catcagttta ggtttttgct atttaacgtt gcgcaaaagt ccccgactct
     9721 ccctctggca ggcgcgaaaa gtgatctcca ttattcatca atccggttta ttacaaacgt
     9781 tagaagtcgg agaaaacctg atcaccgcca gtcatgcatt actgccaggc tggactattc
     9841 cgcactggca agtaccggat gaagtcaaac taccgaaaac cttgacgctg gtttatcacc
     9901 taccgataga acttcatacc atggcagaac gcctacaggc gacactggca gcggaaggct
     9961 gtgaactcac aattattttt cataacgcaa aaaactggga cgacacgacc ctactggcac
    10021 acgcagacct catgatgggc gacagattaa ttggcgaagc accggaatat actctggagc
    10081 aatggctacg ttgcgatcca ctatggccac atgttttcga cgctccagca tatgcacatc
    10141 tgcaatcgac gctggacgcg gcgcaagtaa tgcctgatga ggaaaaccga tttaatgccc
    10201 tgaaagcggt ttttagccag ttaatggcag acgcgacgct gacgccgctg ttcaactatc
    10261 actatcgcat tagtgcccct cccggcgtga acggtgtgcg actgacaccg cgcggctggt
    10321 ttgaatttac cgaagcctgg cttcccgcgc cgtcgcaatg aatagctggt ccgggttaat
    10381 cgcaggcgtt accataacgc ttttattgtt caagcaggat tatctatgaa acgtgctgtc
    10441 gttgtgttca gtggaggcca ggattccacc acctgtctgg tgcaggcatt acaacaatat
    10501 gatgaagtcc attgtgtgac gttcgattac ggtcagcgac atcgcgcaga aatcgacgtg
    10561 gcacgcgaac tggcgctgaa actgggagca cgcgcgcata aggttcttga tgtaacgctg
    10621 ctcaacgagc tggcggtcag cagcctgaca cgtgacagca ttccggtgcc tgattatgaa
    10681 cctgaagccg atggcatccc gaatacgttt gtcccagggc gtaatatttt gttcctgacg
    10741 ctggcggcaa tatatgcgta tcaggtaaaa gcagaagccg taattaccgg cgtctgtgaa
    10801 acggatttct ccggctaccc ggattgccgc gatgagtttg tgaaagcact aaaccatgcc
    10861 gtcagtttgg gcatggcgaa agatattcgt tttgaaacgc cgctaatgtg gattgataaa
    10921 gcggaaacct gggcgctggc agattattac ggcaaactgg atttagtccg taacgaaacg
    10981 ttgacctgct ataacggcat taaaggcgac ggttgcggtc attgtgcggc atgtaattta
    11041 cgcgccaacg gtttgaatca ttatctggcc gataaaccga cggtgatggc agcgatgaag
    11101 cagaaaaccg ggttgaggta attattccgg gtgtcgccgg atgcggcttg agcatccggc
    11161 accacaaaac gtttacttaa ccatctgctc cagcttttcg cgcaattccc cttccagagc
    11221 taatgctttc tgcgttttaa gatcaataca aacaaacgta ataagcgcat ccgctaccac
    11281 ctgcccttcc ggctccagtg taatgaccta gcttaagatg ccgcttttac cgtttaattg
    11341 ctgcaactga ctggtaatag ttaacaggtc gcttaatacc gctgggcgac gatagttaat
    11401 attgatattg accacgacga aggcgatgtt atgggccgtc atccactgaa aactgtcgct
    11461 attttccaac ccatcccagc gggcttcttc gagaaattca aggtagcggg cgttgttgac
    11521 gtgctggtaa acgtcgagat gatatccacg aactttgatt tgtgtttgca tagcgcaata
    11581 accttacgtt gttgttataa gtacagaggt cataactggt atgacctctt cagtctggca
    11641 aaaaattgcc actatgcaaa ttaattacag ggttaatacc gccagattac gttccaccag
    11701 cgaattgccc atccccggca cctgctttag atcctccacc gttttaaacg gaccgtactc
    11761 ttcacgataa ctgacaatcg cctgcgcttt cttcaggcca acgccattca tcgcgcgggc
    11821 tagctcttcc gcgctggcat tattaatgct gacccgggtg ccttcttcgt cactggcttt
    11881 cgccggtact gctgctttac tttgtgctgc aggagcttcc gctttggttt ctaccgccgt
    11941 cggtttcgcc acagaagctg ccgccagcgc gctatgagac attccggcac aggccaggga
    12001 cagggtaatg agcagtgctt taattccgtg tttcatactg ttttctcctt gtttgttaac
    12061 agtggaatca ccttaacggg agtgaaaaca gtaaacaaat ggcaatgttc agaaatggaa
    12121 aaggccgcga aagcgacctt ttcttgttac agctgcaacg catttttttg cgaggcggct
    12181 tcaggattat tgctgttcca gcgcatcgcc aattttgatt ttcgcctctt tacgcaggtt
    12241 actcatcaga gcttcaaaga cgatttgtgc gttgttctgg gtgataccct gcaccatcgc
    12301 ttttttctga tcttccggca ttgaaccttg tttcacttca tccagcgcca gcagaaccac
    12361 attaccttgc atatcggtcg ccataccgta gctcggtttg tctttcgctg gcagtggcag
    12421 tgcaaacgcc gcctggctaa tcgggtcacg accggaacgg cttaaggttt tcggctcgcc
    12481 aaatttcaga ccggcagcct gcatagcttc cgcacctttg ccggctttca aatcaaccag
    12541 cagtttctca gcatccactt tcgcctgttg ttcagctttg ttgtgctgaa ccagtgcctt
    12601 aacttgttcc tgaacatctg ccaacggttt caccgcttcc ggtttgtgct cgctgacgcg
    12661 cagcacgaat gcgcggtcgc cgtctacggt gatgatgtca gagttgatgc ccgacgcgcc
    12721 gttttcacct accagaccgc cgttaaagat agcgtcggca accggcttga agttcaactc
    12781 ttccggcagg ttatctttgc tgaaccaacc cgtctgagtg gctttaacgc cggcagcttg
    12841 ctctgcaccg gccagagact cggtttcgtt gcttgccgca tcgctcactt tctgctgcag
    12901 cgcatagtac gcatcgaggg ctttttcgtg tttcactttc gccgcaatgt cgtcacgtac
    12961 ttcgtctaac gatttcactt tcgctggctg aatgtcgtcc agacgtacaa tcaggaaacc
    13021 gaccgaagat ttgatgacac cagacagttg gcctttttct ttcagaccag catttttcag
    13081 ttcgtcgggg atagtggcat cttctaacca acccatatcg ccgccgttac gggcagagat
    13141 aatatcggca gatttttctt tggctaatgc agcaaaatca ccgcctttat tcagctcatc
    13201 aagtaccgct ttcgcttcat cttcagtttt ggtctggatg atgctgtagc gggtacgctg
    13261 cggctgggtg aattgatcct gatgctgatc gtagtagctc tggatatccg catcgctaac
    13321 cggttgctgc atcgttgcgg caaccagctt gatgtaactc acgcggaatt gttccggcgt
    13381 catgaaattg tttttgtttt gttcgtagta gctggcaatc tcctgttcgg tcacaggctg
    13441 cttcgccgcc agcgcgttaa catcgatagt cgcctcacgc accacgcgtt gttgcgcgac
    13501 cagtgccgcc agctcgtcgg tttcaccttt cagcataaaa tcggtaccgg caacgccgtt
    13561 aatcagctgt tgggtagtga gctggttacg cagcgcctgg gcgtactgat cggcggtcat
    13621 ccccatctgg ttgaggatac cgttatagcg gctgttatca aatttgccgt caacctggaa
    13681 ggctggggtc gcgaaaatcg cctgtttaac ctgctcatca ctgataccca gtttcagctc
    13741 acgagcgtac tgatccaaca gcgcctcgtc gatcagacga ttcagcacct gttgacgcag
    13801 ggttttcata tagccttcgt tcgctgccag ctcggagtat tgatcgccca gctgttgctg
    13861 catgcgatta cgctcgctgt taaaggcgtt ctcgaactgc ccacggctga tttcctggtc
    13921 attcactttt gcggcgtagt tattgcctcc gccaatcagg taaccactca cgccggtcaa
    13981 tatgaacgac acgataatga taccgaaaat aatcttgagc acgagactgt ttgcagccgt
    14041 gcgtaagctg tccatcatgg tgtaacaaca ctccgctgta gatgactgtg tacctcacgc
    14101 aacgtatcga tgcgcgcaaa ggcctattgt gacaagaaac gggggcaatt gtcagcccac
    14161 aagacacaga aagtcaccga atacaaataa aaaaggcaca tcagtagatg cgcccttgaa
    14221 cttcgtcaca tccccactgg ggacaacgct tagtttaccg cgtctttcag tgctttacct
    14281 gcacggaagc tcggtacttt agcggcagcg atggtgatct ctttaccggt ctgcgggttg
    14341 cggccagtac gggcagcacg ctctttaacg gcaaaagtac caaaacctac cagtgctaca
    14401 tcatcccctt ctttcagaga ttcagttacg gaagcaataa tagcatctaa cgcacggcca
    14461 gccgcagctt tagagatatc agcccctgca gcaatcttgt cgatcaattg agatttattc
    14521 actcttctct tcctctttat aatttatatc gcacttgaat ccttcaaggt acgaacgcgc
    14581 agcagttata tcaggccagc catcccctta caacacccct taataagggc aagcccgatc
    14641 cgctatctaa cttagcgaga caaaaaaagg ctggcaagcc cgaattagcc tgccagccct
    14701 gtttttatta gtgcattttg cgcgaggtca ctattttgca gtcacaacct gcatgccaga
    14761 cggttcattt tgcagcgcca gagtcagaac ttcctcaatg cgcttcacag gatgaatgtc
    14821 cagatcggca attacgttgt caggaatctc ttccagatcg cgtttatttt cgaacggaat
    14881 tagcactgtt ttaatcccgc cgcgatgcgc tgccaggagt ttttctttca aaccaccgat
    14941 cggcagtacc tgaccacgca gagtgatctc accggtcatt gccacatcgg cacgaaccgg
    15001 gttaccggtc aggcaagaaa ccagcgcggt gcacatagca ataccggcac tcggaccatc
    15061 tttcggcgtc gcaccttccg gtacgtggac gtggatgtca cgtttttcgt aaaaatcagg
    15121 gttgatcccc agtttttccg cacgcgcacg aaccaccgtt aacgccgcct gaatagactc
    15181 ctgcatcact tcgccgagcg aaccggtata ggtcagtttg cctttacccg gaacacatgc
    15241 ggtttcaatg gtcagcaagt caccgcccac ttccgtccac gccagaccgg ttacctgacc
    15301 gacacggttt tcgttatcag cgcgaccata gtcgaaacgc tgaacaccga ggtagtcatg
    15361 caggttatcg ccgttaattt cgatatgttt taatgactta tcgagcagta actgcttaac
    15421 cgctttgcgg cacagtttgg agatttcacg ctccagacca cgcacgcccg cctcacgggt
    15481 gtagtaacga ataatgccga taatggcgct atcgtcgacg gtcagctcac cttttttcag
    15541 tgcattacgt tcaatctgct tcggcagcag gtgacgtttg gcgatgttca gtttttcatc
    15601 ttcggtataa ccggagaggc gaatcacttc catacgatcc agcagcggtg ccggaatgtt
    15661 catggagttc gacgtcgcga caaacatcac gtcgctgaga tcgtaatcca cttccaggta
    15721 gtggtcgctg aacgctacgt tctgctctgg atccagcact tcaagcagtg cagaggccgg
    15781 atcgcctcgc atgtcagaag acattttgtc gatctcatcg agcaggaaca gcgggttttt
    15841 cacgcccact ttcgccattt tctggatcag tttacccggc atagaaccga tgtaagtacg
    15901 gcggtgacca cggatttccg cttcatcacg cacgccgccc agcgccatac ggacatattt
    15961 acgcccggtg gctttggcaa tggactgacc aagagaggtt ttacctaccc ccggcggccc
    16021 taccaggcag aggatcggtc ccttgatttt gttgacacgg ctttgaaccg caaggtactc
    16081 aaggatgcga tctttcacgc gctccagacc ataatggtcg gtatcaagga tttcctgcgc
    16141 ctgacgcagg tcttttttga ccttgctgcg cgcattccac ggtacctgta ccatccagtc
    16201 gatataacca cgcactacgg tcgcttctgc cgacatcgga gacatcattt tcagcttctg
    16261 caactctgct tccgctttct cttttgcctc tttcggcatc ttcgccgcgt cgattttgcg
    16321 cttcagggct tcgttttcgt ccggcgcgtc gtccatttca ccgagttctt tctgaatagc
    16381 tttcatttgc tcgttcagat agtactcacg ctgggatttc tccatctgct ttttaacgcg
    16441 gttgcgaatg cgtttctcaa cctgcagcag atcgatttcc gattccatca ttgccatcag
    16501 atattccaga cgttcgttaa cgtcggacat ctccagaacg gactgtttgt cagccagttt
    16561 cagcggcata tgtgcagcaa tggtatccgc cagacgcgcc ggatcgtcga tgctattcag
    16621 cgacgtcagc acttctggtg ggattttttt gttcagcttg atgtagcctt cgaactggct
    16681 gattgcagta cgcaccagca cttcctgttc ccgctcatca atggtcggcg actccagata
    16741 ctccgccttc gcagaaaagt gttcgccatt gtcagagagc gcagaaatac gcgcgcgctg
    16801 taacccctcg accagcactt tgactgtgcc gtcaggcagt ttcagcatct gcaatataga
    16861 ggccacggtc ccgacggtga aaagatcgtt tacacccggc tcatccgttg aagcttcttt
    16921 ctgcgcgacc agcataattt ttttatcatg gtccatcgcc gcttccagac aacggataga
    16981 tttttcccgc ccgacaaata aggggatgac catgtgcgga taaaccacca catcgcgcag
    17041 cggcaatacg gggatttcaa tgcgttcaga acgctcagga ttcatagagc tctctcttag
    17101 tttaatttcc gccaggtaat cagatgacac gactgtgctt cacgccatct attaacatgt
    17161 acgtcagtat atggggatgt ttcccccaca ttcaacgccg agaatagagg aaaaattaaa
    17221 ggggagataa aatcccccct ttttgtttaa ctaattgtat gggaatggtt aattattcac
    17281 cagatgcctg ttgcgcttcc ggcttaccat aaatcagcaa cggtttgctt tgaccatcaa
    17341 ttaccgactc gtcaataacc actttttcga cgtcttccat ggacggcaga tcgtacatgg
    17401 tatcgagcag tgcggcttct acgatggaac gcaggccacg ggcaccggtt ttacgcgcca
    17461 tcgctttctt agcgatagca tccagcgcct cgtcacggaa ttccagatcc acgccttcca
    17521 gattaaacag cgcctgatac tgcttggtca gggcgttttt cggctctttg aggatctgaa
    17581 tcagagcttc ttcgctcagt tcattcaacg ttgctacaac cggcagacga ccaatgaact
    17641 cagggataag accaaacttg atcagatctt ccggttcaac ctgcgccagc agctcgcctt
    17701 cgcttgcttt gtcggacttc gcttttaccg tcgcgccaaa accaatgccg gagccggttt
    17761 ctacacggtg ggaaatcact ttatccagac cggcaaacgc accgccacaa ataaacagga
    17821 tcttagaggt atcaacctgc aagaattcct gctgcggatg tttacgccca ccttgcggtg
    17881 gaacagcagc taccgtacct tcgatcagtt tcaacagtgc ctgctgtact ccttcaccgg
    17941 aaacgtctcg ggtaatggac gggttgtctg acttacgaga aatcttgtcg atttcatcga
    18001 tgtagacaat cccgcgctgc gctttctgta cgtcgtaatc gcacttttgc aacagcttct
    18061 gaatgatgtt ttcaacgtct tcgcccacat aaccggcttc ggtcagcgtg gttgcgtcgg
    18121 ccatggtgaa cgggacgtcc agcaggcgcg ccagcgtttc agccagcagc gttttaccgg
    18181 aaccggtcgg accgatcagc agaatgttac ttttgtccaa ctcgacacca ttgctggtat
    18241 cgccgttgcg cagacgtttg taatggttgt ataccgcgac cgccagcact tttttcgcct
    18301 gttcctggcc gataacgtaa tcgtccaggt ggttgcgaat ttcatgcggc gtcggtagcg
    18361 cactgcgttc acgatgcggt gcaacttctt taatctcttc gcgaatgatg tcgttacata
    18421 aatcaacaca ttcgtcgcag atatacacgg atggaccggc aatcagcttg cgcacttcat
    18481 gctggctttt gccgcaaaaa gagcaataca gcaatttgcc tgagccatct ttgcgtttat
    18541 ctgtcatggg tcaaaacctc ttctttgttc tttgtgccgc acacgacgac gcaaatgcca
    18601 ttctcaggcg caagccgctt acagcgttgt gccgccctag ataagtatag cggcacagtt
    18661 gcgcctctgg catcaattac gatgggtcag aatcgaatcg accagaccgt attccaccgc
    18721 ttcaggggcg gaaaggaagc gatcgcgctc ggtatcacgt tcaatctgtt ctaatgattg
    18781 acccgtatga agcgccataa gttcattcat gcgcccttta actttcagaa tttcacgagc
    18841 atgaatttcg atatcggtcg cctggccctg gtagccgccc aacggttggt gaatcatcac
    18901 gcgcgaattc ggcaggcaaa aacgtttacc ttttgcccct gcggtcaaca agaaagcgcc
    18961 catcgaggcc gcctggccca tacagatggt gctgacatca ggcttgataa actgcatggt
    19021 gtcatagata gacatcccgg cagtgatcac cccgcctggg gagttaatgt acagatagat
    19081 atctttttct gggttttccg cttccaggaa cagcatctgc gccacaatca ggttagccat
    19141 gtggtcttca acctggccag tcagaaaaat gacgcgttcc ttaagtagac gagaatagat
    19201 atcaaaagag cgctcaccgc gtgaggtctg ttcaatgacc atcggcacca gcgccatatg
    19261 gggtgcaaag ttatctcgtt cgccgctgta tgacatttcc gtctcctgga taaaattgaa
    19321 aaaaacctgc tgtaccgatt gtaaccttat ggacggatta tcagctagtc cctgtgttat
    19381 gggtacggca tcaccatatt tcaagcataa caatcttttg ttgttacgct aacaccgaaa
    19441 gaggcttttc gcacggctcc atgcaaaatt catgacaaaa aaaagcccac cacctggcgg
    19501 tgacgggcct ttgtgcgaat ttagcgcgtt atgctgcgta aattacgcct gctggttcat
    19561 cagctcgttg aaagtggttt ctttttcagt cactttcgct ttcgccagta cagcttcaac
    19621 agcctgttct tccagagcaa cattgcgcat gttgtccatc agttctttgt ttttgctgta
    19681 gaactcgata acttctttcg gatcttcgta cgcagaagcc atctcttcga tcaggccttt
    19741 cacgcgctct tcgtcagctt tcagctcgtt ggtgcggata acttcgccca gcagcaggcc
    19801 aacaactacg cggcgtttag cctgttcttc gaacagttcg cgcggcagtt ccagagcttg
    19861 tttttcgttg ccaccgaaac gctgtgcagc ctggcgacgc agaacgtcga tttcgctgtc
    19921 gatcagcgca gccggtacgt cgatgtcgtt agcttttacc agaccttcga tcgcctgaga
    19981 cttaacgcgg ttacggatgg cgctcttcag ctcgcgctcc atgtttttac gcacttcagc
    20041 gcgcagacct tctacggaac catcttcaac gccgaaacgt ttgatgaatt ctgcagtcag
    20101 ttccggcagt tcacgctctt caactttctt caggttgata gcgaatttcg ctgctttacc
    20161 tttcaggttt tctgcgtggt attcttccgg gaaggtcacg tcgatggtga actcttcgcc
    20221 agctttgtgg cctttgatac cgtcttcaaa gcccgggatc atacgaccct ggcccatcgc
    20281 cagtacgaaa tcagacgctt taccgccttc gaactcttcg ccgtctacag aaccggtgaa
    20341 gtcgatggtt acgcggtctt ctgcttcaac agcgccgtct ttttctttcc aggtcgcctg
    20401 ctgtttacgc agagtatcca gcatgccgtc aacgtcagcg tcggtcactt caacgatcgg
    20461 tttttcaact tcgatcgctt ccagaccctg cagttcaact tccggataaa cttcaaactc
    20521 tacagagtaa gtgaagtctt cacccagctt gtattcgccc ggaacataag tcggtgcgcc
    20581 agccggattg attttttctt taatgatggc gtcaatgaag ttacggctca tcaggtcacc
    20641 cagaacgtcc tggcgtacag acgcgccata acgctgagca acgatattca ttggcacttt
    20701 gcctttgcgg aagccgtcaa tacgtacttt tttcgcaacg ttgaccagct cgcttttaac
    20761 agcggtctcg atgctgtcag cagcgatagt aatcgttaca cggcggccaa ggccttgagt
    20821 ggtttcaact gaaacttgca tcttgttacc tcaaaaaatc acagtgctcg gtcaactctt
    20881 accccgcaag cacaggttgt tggcttcgcg gaaccgggat gttcttataa tcaatcacat
    20941 tccctgttac cagaatcatc ccgaaagcat tcaaatagga cgcggcatta tagcggcatc
    21001 acttttatga gtcgagaacg gttattgcgc gctgtcgcgt attttttcac aattcccggg
    21061 caattttcgt ctgaatgctg ggcaaaaagc gcaaaatcca gacaaaacaa aacggcccgc
    21121 aggccgtttt tcataaaatc tttagcaaca tactggaaaa gcgccgacag ttgcaaatgc
    21181 gtttttacgc gatgcttcct gctccacgac agggaggaga ggcaaagacg gtgtcctgca
    21241 acccttccca ctccttaata gtgtaagtat gcagagccag cgcatgaacg gtagtagaga
    21301 gttcctccgc taaagtactg taaatcattc gatgacgatt cagaaaacgt tcacccgtaa
    21361 aacgatcgct gaccagcaca actttaaaat ggctttcaga gccggctggg acattgtgac
    21421 gatagctttc atccactact tcgaggaata cgggttggaa cgccgccctt aatttttctt
    21481 ctatccgctc acgtatcatc atgaaatatc tcctccgaca acgctgagat gtcatccatc
    21541 cctttaaata ctagccgctt ttaccgtttc cattgcagct taacaacaaa tatttaggtt
    21601 tcgtcctggt ttttccgtca agcgtatgaa gtttactgat ttaaagattg aaacacaggc
    21661 ctcgcatcag cgtttctttt accaggggtg attatttcgt caacatgatg aatttacatg
    21721 ccttacccac ttcccctcgc cgttggcaat gttatgatgg cgggaatttt taccaaacac
    21781 gcttaagagt ctctgagagc ccgaacatgt tcaaaaaaat cctcttcccg ttagttgctc
    21841 tgtttatgct tgcaggatgc gcaaaaccgc caacaactat tgaagtttcc ccgacgatta
    21901 cactgccaca gcaggatcca agcctgatgg gcgtcaccgt aagcattaat ggtgccgatc
    21961 agcgtacaga tcaggcgctg gcaaaagtca cccgcgataa tcaaatcgtt accctgaccg
    22021 cctcccgcga tctgcgtttc ctgctgcaag aagtgctgga aaaacagatg accgcgcgtg
    22081 gttacatggt tggtccgaat ggcccggtta atctgcaaat catcgttagc caactgtatg
    22141 ctgacgtgtc ccagggcaac gtgcgctaca acatcgctac caaagcggat atcgccatca
    22201 tcgctaccgc gcagaatggc aacaaaatga ccaaaaacta tcgtgccagc tacaacgttg
    22261 aaggtgcgtt ccaggcctcc aacaaaaata tcgccgatgc ggttaacagt gtgctgaccg
    22321 ataccatcgc tgatatgtct caggacacca gcattcacga attcatcaag cagaacgcgc
    22381 gttaatttct gccctctggc ccggtgcaag ccgggcctgt agacgcccat gtccagtcaa
    22441 tatttacgta tttttcaaca gccgcgttca gccatattgc tgatcctggg ttttgcttcc
    22501 gggctaccgc tcgccctgac atccggcacc ttacaggcct ggatgacggt cgaaaatatc
    22561 gatctcaaaa ccattggttt cttctctctg gtaggccagg cttacgtttt taaattcctc
    22621 tggtcaccgc tgatggaccg ctacacgccc ccattttttg ggcggcggcg cggttggctg
    22681 ctcgccacgc aaatcctgtt attagtcgcc attgcggcga tgggttttct cgaaccaggc
    22741 acccaactcc gctggatggc ggcgctggcg gtggtgatcg ctttttgctc tgcctcccag
    22801 gatattgtct tcgatgcgtg gaaaaccgat gtgctcccgg cagaagaacg tggtgcgggc
    22861 gcggcaatca gcgtgctggg ttaccgttta gggatgctgg tttccggcgg cctagccctg
    22921 tggctggcag ataaatggtt gggctggcag ggcatgtact ggttgatggc ggcgctgttg
    22981 atcccctgta ttatcgcgac gttgcttgca ccagaaccaa ccgacaccat tcctgtgccc
    23041 aaaacgctgg aacaagcggt tgttgcacct ctgcgagatt tctttggtcg caataatgcc
    23101 tggcttattt tgcttcttat cgtgctgtat aagctgggcg acgcattcgc catgagcctg
    23161 acaaccacgt ttttgattcg cggcgtcggg tttgatgcgg gtgaagtagg cgtggtaaac
    23221 aaaacgcttg gcttactggc gaccattgtt ggcgcattgt acggtgggat tttgatgcag
    23281 cgcctgtcgc tgttccgggc gctgctgatt ttcggcattt tacaaggtgc gtctaacgct
    23341 ggttactggc tgctgtcgat tactgataag catctctaca gcatgggcgc agccgtcttt
    23401 ttcgaaaacc tctgtggcgg gatgggcaca tcagcctttg tcgcgctgtt aatgacgctg
    23461 tgtaataagt cattttccgc tactcaattt gccctgctct cagcgctttc cgctgtaggg
    23521 cgagtttatg tcggccccgt ggcgggttgg tttgttgaag cacacggctg gtcgacattc
    23581 tatctattct ccgtcgccgc tgccgtaccg gggcttattt tgctgctggt ttgccgccag
    23641 acgcttgaat atacacgagt aaatgacaac tttatctccc gtaccgaata tccggcaggt
    23701 tatgcctttg ccatgtggac actggcggcg ggcgtcagcc tgttggccgt gtggttactt
    23761 ctgttgacga tggacgcgct ggatttgacg cacttctctt tcctgcctgc tctgctggaa
    23821 gtcggggttt tagtcgccct ttctggcgtc gtgcttggtg gtttgctgga ttatctggcg
    23881 ctacgaaaaa cgcatctgac gtaatctgta aatattattt agctgtagta atcactcgcc
    23941 gtaattatta cggctaaata aacatcagca tcgcttatta atatttgttc ttttgtgcgg
    24001 cttagcgttt ggaaattatt ggcgccattt ataaattcta tttttcttac acgattcagc
    24061 taatgagtct ttattttctc accacccagt tgtcactcta atgataatta tttgctaaat
    24121 aattgtttta tttcgcattg gttataccaa ttgcccgcca agactccttc tgcactcccc
    24181 ttttgtta
//
LOCUS       DADMEQ010000055        24069 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.055, whole genome shotgun sequence.
ACCESSION   DADMEQ010000055 DADMEQ010000000
VERSION     DADMEQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.055"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..188)
                     /locus_tag="JNZ05_002211"
     CDS             complement(<1..188)
                     /locus_tag="JNZ05_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565434.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITT"
     gene            259..324
                     /locus_tag="JNZ05_002212"
     CDS             259..324
                     /locus_tag="JNZ05_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565435.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            463..1074
                     /gene="ruvA"
                     /locus_tag="JNZ05_002213"
     CDS             463..1074
                     /gene="ruvA"
                     /locus_tag="JNZ05_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAY8565436.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            1083..2093
                     /gene="ruvB"
                     /locus_tag="JNZ05_002214"
     CDS             1083..2093
                     /gene="ruvB"
                     /locus_tag="JNZ05_002214"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAY8565437.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(2240..3025)
                     /gene="znuB"
                     /locus_tag="JNZ05_002215"
     CDS             complement(2240..3025)
                     /gene="znuB"
                     /locus_tag="JNZ05_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HAY8565438.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(3022..3777)
                     /gene="znuC"
                     /locus_tag="JNZ05_002216"
     CDS             complement(3022..3777)
                     /gene="znuC"
                     /locus_tag="JNZ05_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HAY8565439.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3856..4788
                     /gene="znuA"
                     /locus_tag="JNZ05_002217"
     CDS             3856..4788
                     /gene="znuA"
                     /locus_tag="JNZ05_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HAY8565440.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4804..6126
                     /gene="mepM"
                     /locus_tag="JNZ05_002218"
     CDS             4804..6126
                     /gene="mepM"
                     /locus_tag="JNZ05_002218"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HAY8565441.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            6246..7217
                     /gene="lpxM"
                     /locus_tag="JNZ05_002219"
                     /gene_synonym="msbB"
     CDS             6246..7217
                     /gene="lpxM"
                     /locus_tag="JNZ05_002219"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY8565442.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(7348..8790)
                     /gene="pyk"
                     /locus_tag="JNZ05_002220"
     CDS             complement(7348..8790)
                     /gene="pyk"
                     /locus_tag="JNZ05_002220"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAY8565443.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMHADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(8918..9787)
                     /locus_tag="JNZ05_002221"
     CDS             complement(8918..9787)
                     /locus_tag="JNZ05_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY8565444.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            10125..11600
                     /gene="zwf"
                     /locus_tag="JNZ05_002222"
     CDS             10125..11600
                     /gene="zwf"
                     /locus_tag="JNZ05_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAY8565445.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            11835..13646
                     /gene="edd"
                     /locus_tag="JNZ05_002223"
     CDS             11835..13646
                     /gene="edd"
                     /locus_tag="JNZ05_002223"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HAY8565446.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLFRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVRTGRELFSALREKLSGAEQGATCITF"
     gene            13683..14324
                     /locus_tag="JNZ05_002224"
     CDS             13683..14324
                     /locus_tag="JNZ05_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAY8565447.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(14379..15557)
                     /gene="purT"
                     /locus_tag="JNZ05_002225"
     CDS             complement(14379..15557)
                     /gene="purT"
                     /locus_tag="JNZ05_002225"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HAY8565448.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            15691..15981
                     /gene="yebG"
                     /locus_tag="JNZ05_002226"
                     /pseudo
     CDS             15691..15981
                     /gene="yebG"
                     /locus_tag="JNZ05_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            16048..16404
                     /gene="yebF"
                     /locus_tag="JNZ05_002227"
     CDS             16048..16404
                     /gene="yebF"
                     /locus_tag="JNZ05_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HAY8565449.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDATGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            16731..17390
                     /locus_tag="JNZ05_002228"
     CDS             16731..17390
                     /locus_tag="JNZ05_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HAY8565450.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            17599..19659
                     /gene="ptrB"
                     /locus_tag="JNZ05_002229"
     CDS             17599..19659
                     /gene="ptrB"
                     /locus_tag="JNZ05_002229"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HAY8565451.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQHWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPT
                     TPAD"
     gene            complement(19656..20318)
                     /gene="exoX"
                     /locus_tag="JNZ05_002230"
     CDS             complement(19656..20318)
                     /gene="exoX"
                     /locus_tag="JNZ05_002230"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HAY8565452.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(20342..20998)
                     /locus_tag="JNZ05_002231"
     CDS             complement(20342..20998)
                     /locus_tag="JNZ05_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HAY8565453.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGNYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(21100..21330)
                     /locus_tag="JNZ05_002232"
     CDS             complement(21100..21330)
                     /locus_tag="JNZ05_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAY8565454.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            21469..21843
                     /gene="yobA"
                     /locus_tag="JNZ05_002233"
     CDS             21469..21843
                     /gene="yobA"
                     /locus_tag="JNZ05_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HAY8565455.1"
                     /translation="MASTARSLRYALTILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            21847..22719
                     /gene="copD"
                     /locus_tag="JNZ05_002234"
                     /pseudo
     CDS             21847..22719
                     /gene="copD"
                     /locus_tag="JNZ05_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            22732..23073
                     /locus_tag="JNZ05_002235"
     CDS             22732..23073
                     /locus_tag="JNZ05_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HAY8565456.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(23466..>24069)
                     /locus_tag="JNZ05_002236"
     CDS             complement(23466..>24069)
                     /locus_tag="JNZ05_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8565457.1"
                     /translation="RISQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPAWFPLA
                     MDLALVTGQRREDITNMRFSDIYDDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGAVVE
                     QCRMVSRGDYLISAGIRKNSPDGSIHPDGLTKKFVAARKLTGILFSENPPTFHEIRSL
                     AGRLYKEICGEEFAQRLLGHTSEKTTKMYLDEREKTYLLL"
BASE COUNT         6109 a         5932 c         6141 g         5887 t
ORIGIN      
        1 gtggtgatag ttgaaagggg aacccggctt tctgcaacca ggaagtcttc accgttatga
       61 ccgataatga tcccgacgtg attactccag caatttgatg cagctgatat ttgaccaaat
      121 aaggcagcac ctatacatgt aaagacgata tctccaattt catattcagc agggtaatta
      181 atattcacgt tgtcattcca ttgaaataga tacgaatata cgtaatagca taaaaaaatt
      241 attttttcag gagtcgcaat gtatattttt attacgcatt tcttcactga atatgtaata
      301 ttcaaatatt tgcttccaat ataacctgtt gaataaatta tactgtgcca tttttcagat
      361 catcgggaca cctcgcaagt tttcttcatc ctacgctgga tatctatcca gcattttttt
      421 atcatacagc attatctttg attcattacg caggagcgtc atgtgatagg cagactcaga
      481 ggcatcatca ttgaaaaaca acccccgctg gtgttaattg aagtgggcgg cgtaggctat
      541 gaagtgcata tgccgatgac ctgtttttat gaactccctg aagcgggtca ggaagcgatc
      601 gttttcaccc actttgtggt gcgtgaagac gcgcaactgc tgtacggttt taacaataaa
      661 caagaacgca cgttgttcaa agagttgatc aaaaccaacg gcgtcggccc gaagttggcc
      721 ctggcgatcc tctccggaat gtcagcgcag cagttcgtta atgccgttga gcgtgaagaa
      781 gtgggggcac tggtgaaact gccgggtatt ggcaaaaaaa ccgccgaacg cttgattgtt
      841 gaaatgaaag accgatttaa aggtttgcat ggcgatctct ttacgccagc cgccgacctg
      901 gtactcacgt ctcctgccag cccggcgacc gacgatgctg aacaagaagc agttgccgcg
      961 ctggtggcgc taggctataa accacaagaa gcaagccgca tggtgagcaa aatcgctcgc
     1021 cctgacgcca gcagtgaaac attaattcgc gaagccctac gcgccgcgtt atgaggtaaa
     1081 ggatgattga agcagaccgt ctgatttctg ccggtaccac tttgccggaa gatgtagcag
     1141 atcgcgccat tcgccctaaa ttactggaag agtatgttgg tcagccgcag gttcgttcac
     1201 agatggagat tttcatcaaa gcagcgaaac tgcgcggcga tgccctcgat catttgttga
     1261 tttttggtcc tccggggttg ggtaaaacta cgcttgccaa cattgtcgcc aatgaaatgg
     1321 gcgttaattt acgcacgact tctggtccgg tgctggaaaa ggcgggcgat ttggctgcga
     1381 tgctcactaa ccttgaaccg catgacgtgc tgtttattga tgagatccac cgtttatcgc
     1441 cagttgttga agaagtgctg tacccggcaa tggaagacta ccaactggat atcatgattg
     1501 gtgaaggtcc ggcggcacgc tccattaaaa ttgatttgcc gccgtttacc ctgattggcg
     1561 caaccacgcg cgcaggttcg ctgacatcac cgttgcgcga ccgttttggt attgtgcaac
     1621 gtctggagtt ttatcaggtg ccggatctgc aatatatcgt cagtcgcagt gcacgcttta
     1681 tggggcttga gatgagtgat gacggtgcgc aggaagtagc tcgtcgcgct cgcggtacgc
     1741 cacgtattgc caaccgtctg ctgcgtcgag tgcgtgattt cgccgaagtg aagcacgatg
     1801 gcaccatctc ggcagatatc gctgctcagg cgctggatat gttgaatgtc gatgctgaag
     1861 gtttcgatta tatggaccgc aaattgttgc tggcggtaat cgataagttc tttggtgggc
     1921 cggtaggtct ggataacctg gcggcagcca ttggcgaaga acgtgaaacc attgaagatg
     1981 tactggaacc ttatttgatt cagcaaggct ttttgcagcg tacaccgcgc gggcgtatgg
     2041 cgacgacgcg ggcgtggaat cactttggca taacgccgcc agaaatgccg taagtcggat
     2101 tggtcgctcg cgccgcatcc gacactggca gtgtgtctga tgcgacactg ttcgcgtctt
     2161 atcaggccag ccagagatag cgaaccgtag gtcggataag gcgctcgcgc cgcatccgac
     2221 aaatgtgttc agcgatagat tagctggcct gctttttcat catactgata ataaataaca
     2281 gtgccgcaca tagcaccacc gacggacccg ccggcgtatc gtaaaacgcg gaaaaggtta
     2341 aaccgccagt tactgccacc atccccacca aaacagcgac accagccatc tgttccggcg
     2401 tacgggcaaa gcgacgcgca gtagctgcag gaataatcag caacgaagta ataatcaacg
     2461 caccgacaaa tttcatcgct acaccaatcg tcaatgccgt caccagcatc aacaacaatt
     2521 tcacttgctg taatttcaca ccatcaacaa acgccagatc cgggctaatc gtcatcgaca
     2581 gcaaattgcg ccattgccag aacaaaatag ccaccacgat gaccacgcca atcgcaatag
     2641 agatgagatc ttctggcgtc actgccagta aatcaccgaa caggtaagcc atcaaatcaa
     2701 cacgaatgtt agacatcaga ctaacgacca ctaggcccag cgacagggca ctgtgcgcca
     2761 taatccctaa taacgtgtcg atcgccagct gtggacgctt ctccagccat accagaccgc
     2821 ccgccagcag cagcgtaacg gcaatcaccg catagaatgg attcacgtcc agcaacaaac
     2881 caaacgcgac accaagtaat gaggcatgag ccagcgtatc accgaaataa gacatacgac
     2941 gccagactac aaacgaaccc agcggacccg cggcacaggc gagcatgatc ccggctaacc
     3001 aaccgggaaa taataattca atcatgagcg atcatttccc cgacgcaaaa caattcgtcc
     3061 ctgtaaatcg tgacgatgat tatgatgatg gcgatagata cccagttgtt cagcaccacg
     3121 aggaccaaac attgaaataa actccggatg cagggaaaca acttccggtg tgccggaaca
     3181 acaaatgtgg tgattcaggc acagcacttc atcggttttt gccatcacca gatgcagatc
     3241 gtgagaaacc attaaaacgc cacaatccag ttcacgacgc aactggtcaa taaggtcata
     3301 taacgccact tggccattta catccacgcc ttgagtgggt tcatccagca ctaataattg
     3361 cgggcaattt aacaatgctc gcgctaatag tacacgctgc gtttcgccac cagagagctt
     3421 ttgcatcggt gcgttaatca ggtgcccggc ctggacacgt ttcagtgcag gcaaaatatc
     3481 ttctttatgt gtaccagggc gtaagcgtaa aaaacggttt acggtcagtg gcaacgtggt
     3541 gtcgagatac agcttctgcg gtacatagcc gatgcgcagt tttccgttgc gcttgataac
     3601 cccttcatcg ggtgttacca gcccgagcac tacccgtacc agtgtcgact tacctgcgcc
     3661 atttggccca agtaaagtca aaatttttcc aggtttaagt tccagcgaca catcagagag
     3721 gacgcggcgt tggccaaaag aaaccgagac attttccagg gaaaccagac ttgtcatgtt
     3781 aattttagtc ttgcagtagt catgaaatgt tataatatca cacttctcat attcattacg
     3841 atgattggtc gcattatgtt acataaaaaa acgcttcttt tcgcagcatt atccgccgct
     3901 ctctggggag gtgcaacaca ggccgcagat gccgccgttg tcgcttcgct taaacccgtt
     3961 gggttcatcg cttctgccat tgctgatggt gtaacagaaa cggaggtttt actgcctgac
     4021 ggcgcttcag aacatgatta ttcactgcgt ccatcggatg taaaacgctt acagaacgcg
     4081 gacttagtcg tttgggttgg cccggagatg gaagcgttta tgcaaaaacc ggtaagcaaa
     4141 ttaccagaag cgaagcaggt aacgattgcg cagcttgaag atgtgaaacc gctactgatg
     4201 aaaagtattc acggcgatga tgatgatcac gaccacgcgg aaaaaagtga cgaagatcac
     4261 catcacggcg atttcaacat gcatctttgg ctttccccag agatagcgcg ggctacagcg
     4321 gttgcaatcc atggaaaatt agtggaactt atgccgcaaa gtcgagccaa acttgacgcc
     4381 aacctgaagg attttgaggc acaattggcc tcaaccgaaa cgcaggttgg taacgagctc
     4441 gcgccgctca aggggaaagg ttatttcgtt tttcacgatg cttacggcta tttcgaaaaa
     4501 cagttcggac tgacaccgct tggtcatttt accgttaacc ctgagattca acctggcgcg
     4561 cagcgtttac atgaaataag aacacagttg gttgagcaaa aagcaacctg cgtttttgct
     4621 gagccacagt tcaggccagc ggtcgttgag agcgtcgcac gagggacatc cgttcgtatg
     4681 ggaacgttgg atcccctggg gacgaatatc aaactgggta aaacaagcta ttcagaattc
     4741 ctgagtcaat tagccaacca gtatgcgagc tgcctgaaag gagattaatg aggaagtgat
     4801 tacgtgcaac agatagcccg ctctgtcgcc ctggcgttta ataatttacc gcgaccacac
     4861 cgcgttatgt tggggtcgct caccgttctt actctggccg tcgctgtctg gcggccctat
     4921 gtttatcacc gtgatgccac gccaattgtc aaaaccattg agctggaaca gaacgaaatt
     4981 cgttcgctct tacctgaagc cagtgagccg attgatcaag ctgcacaaga agatgaagcc
     5041 attccccagg acgaactgga tgacaaaatc gccggtgaag cgggcgtgca tgaatatgtt
     5101 gtttccactg gcgatacgct aagcagcatt ctcaatcagt atggtattga tatgggtgat
     5161 atcacccaac tcgctgctgc cgacaaagaa ttgcgtaacc tgaaaatcgg tcaacaactc
     5221 tcctggacat taaccgcgga cggcgaactg cagcgcctca cctgggaagt gtctcgtcgt
     5281 gaaacccgaa cctatgaccg tactgccgct aacggtttta aaatgaccag cgaaatgcag
     5341 caaggagagt gggttaacaa tctgctgaaa ggtaccgtcg gagggagctt tgttgccagc
     5401 gccagaaacg ccggtttaac cagcgccgaa gtgagcgcag tgattaaagc catgcagtgg
     5461 caaatggatt tccgcaaact gaaaaaaggc gatgaatttg cggtattaat gtctcgagaa
     5521 atgcttgatg gtaaacgtga gcaaagccag ctgctgggcg tacgtttgcg ttcagaaggt
     5581 aaagattatt acgcaatccg cgctgaggat ggcaaattct acgatcgtaa cggtactggt
     5641 ctggcgaaag gattcttgcg attcccgacg gcgaaacagt tccgtatctc gtctaacttt
     5701 aacccgcgtc gaactaatcc ggtgacaggt cgcgttgcac cacacagagg tgttgatttc
     5761 gccatgccac aaggtacgcc agtgctttca gtgggtgacg gtgaagtggt ggttgccaaa
     5821 cgcagtggcg cagcaggtta ttatgtggct attcgtcatg gtcgcagcta caccacgcgt
     5881 tatatgcact tgcgcaagat tctggtgaaa ccgggacaga aggtgaaacg tggcgaccgt
     5941 atcgcgcttt ccggtaatac cggacgttca actgggccgc atctgcacta tgaagtatgg
     6001 ataaaccagc aggccgtaaa cccgctgacg gcaaaactgc cgcgtaccga agggctgacc
     6061 ggctccgatc gtcgcgaatt cctggcgcag gccaaagaga ttgtgccgca gctacggttt
     6121 gattaattaa catccattcg cagccggtac gcagtcagta ccggcttttt ttatttggtg
     6181 cggggcaagt tgcgccgcta cactatcacc agattgattt ttgccttatc cgaaactgga
     6241 aaagcatgga aacgaaaaaa aataatagcg aatacattcc tgagtttgat aaatcctttc
     6301 gccacccgcg ctactgggga gcatggctgg gtgtagcagc gatggcgggt atcgctttaa
     6361 cgccgccaaa gttccgtgat cccattctgg cacggctggg acgttttgcc ggacgactgg
     6421 gaaaaagctc acgccgtcgt gcgttaatca atctgtcgct ctgctttcca gaacgtagtg
     6481 aagctgaacg cgaagcgatt gttgatgaga tgtttgccac cgcgccgcaa gcgatggcaa
     6541 tgatggctga gttggcaata cgcgggccgg agaaaattca gccgcgcgtt ggctggcaag
     6601 ggctggagat catcgaagag atgcggcgta ataacgagaa agttatcttt ctggtgccgc
     6661 acggttgggc cgtcgatatt cctgccatgc tgatggcctc gcaagggcag aaaatggcag
     6721 cgatgttcca taatcagggc aacccggttt ttgattatgt ctggaacacg gtgcgtcgtc
     6781 gctttggcgg tcgtctgcat gcgagaaatg acggtattaa accattcatc cagtcggtac
     6841 gtcaggggta ctggggatat tatttacccg atcaggatca tggcccagag cacagcgaat
     6901 ttgtggattt ctttgccacc tataaagcga cgttgcccgc gattggtcgt ttgatgaaag
     6961 tatgccgtgc gcgcgttgta ccgctgtttc cgatttatga tggcaagacg catcgtctga
     7021 cgattcaggt gcgcccaccg atggatgatc tgttagaggc ggatgatcat acgattgagc
     7081 ggcggatgaa tgaagaagtc gagatttttg ttggtccgcg accagaacaa tacacctgga
     7141 tactaaaatt gctgaaaact cgcaaaccgg gcgaaatcca gccgtataag cgcaaagatc
     7201 tttatcccat caaataaaaa agcctctcgc gaggagaggc cttcgcctga tgataagttc
     7261 aagtttgctt cagaatattc gaaatctgtt gaactatcat tgaactatag gccggatgtg
     7321 gcgttttcgc cgcatccggc aacgtactta ctctaccgtt aaaatacgcg tggtattagt
     7381 agaacccacg gtactcatca cgtcgccctg ggtgacaatc accaggtcac cagacatcaa
     7441 gtaaccttta tcgcgcagca gattaaccgc ttcgctggca gctgctacgc cgtcattagc
     7501 gctatcaaag tgcaccggcg taacgccacg atagagagca gtcaggttca gcgtacgttc
     7561 atggcgcgac atggcgaaaa ttggtagacc agagctgata cgggaggtca tcagcgcggt
     7621 acgacccgat tcggtcatgg taatgatcgc cgtaacgccc ttcaggtggt tagctgcgta
     7681 cattgccgac atggcaatag cttcttccac attgtcgaac tgaacgtcca gacggtgttt
     7741 agaaacgttg atgctcggga ttttttccgc acccaggcaa acgcgcgcca tggctgcaac
     7801 agtttctgac ggatactgcc cagcggcagt ttctgcagac agcatcacag cgtcagtacc
     7861 atccagaacg gcgtttgcta cgtccatgac ttctgcacgc gtcggcatcg ggttagtaat
     7921 cattgactcc atcatctggg tcgccgtgat taccgctcgg tttagctgac gcgcacgacg
     7981 gatcaacgct ttctgaatgc cgaccagttc cgggtcgcca atttccacac cgaggtcgcc
     8041 acgtgcaacc attaccacgt cagaggcgag gatgatgtca tccattgcat cctggctgca
     8101 aacggcttcc gcacgttcaa ccttggcaac aattttcgca tcacatcctg catcgcgtgc
     8161 caggcgacgg gcatagttca gatcttcgcc acagcgtggg aaggagacag ccaggtaatc
     8221 tacgccaatc aacgccgcag tcttaatgtc tgctttgtct ttttcggtca gcgcttcagc
     8281 cgacaaaccg ccgccaagtt tgttgatacc tttattgttg gagaggggac caccgacggt
     8341 gacttcggtg aacactttca tgccctgaac ttccagtact tttaactgga cgcgaccatc
     8401 gtccagcagc aggatgtcac caggcacgac gtcagcaggc aggcctttgt agtcgatacc
     8461 gactttttct ttgtcgcctt cacctttacc caggttggcg tcgagcagga atttatcccc
     8521 aatattgagg aaaactttgc cttctttaaa ggtggataca cggattttgg gcccctggag
     8581 gtcacccaga atagccacat gacgccccag ttttgcggca atctcacgaa ctttatccgc
     8641 gtgcattttg tgatcttcag gcgagccgtg agaaaagttc atacgtacaa cgttggcacc
     8701 cgccgcgata actttttcaa gattattatc gcgatctgtt gctgggccta acgtggtaac
     8761 gatttttgtt ctgcgaagcc ttttggacat gtaatactcc gttgactgaa acaaccaggt
     8821 gttgcttgaa catgaaatcc gaatgaaata acgccgcgat ggcgataaat taatctcatt
     8881 cggatgtcgt ataagattag gacagtgaca gtcgctttta gcgatcgtca cttaaattaa
     8941 gtaactgctt atcaaaacgc gattccttca gcgcttcctt gacccgcttc aagttatctc
     9001 tgaattttgc accgcgtcgc aaagtaaatc ctgtcgccag cacatctatc acggtcagct
     9061 gtgcaagtcg agaaaccatg ggcatataaa tgtcagtatc ttccggtacg tcgagggtaa
     9121 ttgccagcgt tgcttcccgg gcgagtgggg tacccgcaga ggtgagggca atcaccatgg
     9181 cgtcgttttc gcgtgccagc tgcgccagct cgaccagatt ttttgttctt cctgtgtgag
     9241 aaatcagcac caccacgtct ccgtcgctac aattcataca actcatgcgt tgcagcacga
     9301 tatcatcgga gtacaccacc ggaacattaa aacgaaagaa cttattcatc gcatcgtggg
     9361 caacggcggc agaagagcct aatccgaaaa aggcgatttt ttttgcctga gtgagcaagt
     9421 cgacggcgcg gttgatggca gatttatcca gtgaatgacg gacatgatcg agcgttgcca
     9481 ttgcggactc aaatattttc cccgtgtatg attcaacgct gtcatcttca ttgacgttgc
     9541 gattaacata gggagtgcca ttcgccagac tttgtgccag atgaagttta aaatcaggaa
     9601 aaccgcgcgt gtccatgctg cgacagaaac gattcaccgt cggttcgcta acattggctt
     9661 ccagcgccag agcagcaata ctcgaatgga tcgcgttatc gggcgaagcc agaatgacct
     9721 cggcaacttt gcgctctgat ttgctcaaat gttccagctg agactggatt ttttccagca
     9781 tattcatgat gtaaagagac tcacgggtaa tgacgatttc cgcactgaaa gaaatcgaaa
     9841 tgcagttttg tcagatatta cgcctgtgtg ccgtgttaat gacaaaagca gataaaaaag
     9901 ttgttatttt ttttcataac atgatcagtg tcagattttt accgaatgga aaacgatgat
     9961 ttttttatca gttttgccgc actttgcgcg cttttcccgt aatcgcacgg gtggataagc
    10021 gtttacagtt ttcgcaagct cggaaaagca gtacagtgca ccgtaagaaa attacaagta
    10081 taccctggct taagtaccgg gttagttaac ttaaggagaa tgacatggcg gtaacgcaaa
    10141 cagcccaggc ctgtgacctg gtcattttcg gcgcgaaagg cgaccttgcg cgtcgtaaat
    10201 tgctgccttc cctgtatcaa ctggaaaaag ccggtcagct caacccggac acccggatta
    10261 tcggcgtagg gcgtgctgac tgggataaag cggcatatac caaagttgtc cgcgaggcgc
    10321 tcgaaacttt catgaaagaa accattgatg aaggtttatg ggacaccctg agtgcacgtc
    10381 tggatttttg taatctcgat gtcaatgaca ctgctgcatt caaccgtctc ggcgcgatgc
    10441 tggatcaaaa aaatcgtatc actattaact actttgccat gccgcccagc acttttggcg
    10501 caatttgcaa agggcttggc gaggcaaaac tgaatgctaa accggcacgc gtagtcatgg
    10561 agaaaccgct ggggacgtcg ctggcgacct cgcaggaaat caatgatcag gttggcgaat
    10621 acttcgagga gtgccaggtt taccgtatcg accactatct tggtaaagaa acggtgctga
    10681 acctgttggc gctgcgtttt gctaactccc tgtttgtgaa taactgggac aatcgcacca
    10741 ttgatcatgt tgagattacc gtggcagaag aagtggggat cgaagggcgc tggggctatt
    10801 ttgataaagc cggtcagatg cgtgacatga ttcagaacca cctgctacaa attctctgca
    10861 tgattgcgat gtctccgcca tctgacctga gcgcagacag catccgcgat gaaaaagtga
    10921 aagtactgaa gtccctgcgc cgcatcgacc gctccaacgt acgcgaaaaa accgtacgcg
    10981 ggcaatatac tgcgggcttc gcccagggca aaaaagtgcc gggatatctg gaagaagagg
    11041 gcgcgaacaa aagcagcaat acagaaacct tcgtggcgat ccgcgtcgac attgataact
    11101 ggcgctgggc cggtgtgcca ttctacctgc gtactggtaa acgtctgccg accaaatgtt
    11161 ctgaagtcgt ggtctatttc aaaacacctg aactgaatct gtttaaagag tcgtggcagg
    11221 atctgccgca gaataaactg actatccgtc tgcaacctga tgaaggcgtg gatatccagg
    11281 tactgaataa agttcctggc cttgaccaca aacataacct gcaaatcacc aagctggatc
    11341 tgagctattc agaaaccttt aatcagacgc atctggcgga tgcctatgaa cgtttgctgc
    11401 tggaaaccat gcgtggtatt caggcactgt ttgtacgtcg cgacgaagtg gaagaagcct
    11461 ggaaatgggt agactccatt actgaggcgt gggcgatgga caatgatgcg ccgaaaccgt
    11521 atcaggccgg aacctgggga cccgttgcct cggtggcgat gattacccgt gatggtcgtt
    11581 cctggaatga gtttgagtaa tatcagcgct tatcctttat ggttatttta ccggtaacat
    11641 gatcttgcac agattgtaga acaattttta cactttcagg cctcgtgcgg attcacccac
    11701 gaggcttttt ttattacact gactgaaacg tttttgccct atgagctccg gttacaggcg
    11761 tttcactcat aaatcctctg aatgaaacgc gttgtgaatc atcctgctct gacaactcaa
    11821 tttcaggagc ctttatgaat ccacaattgt tacgcgtaac aaatcgaatc attgaacgtt
    11881 cgcgcgagac tcgctctgct tatctcgccc ggatagaaca agcgaaaacg tcgaccgttc
    11941 atcgttcgca gttggcatgc ggtaacctgg cacacggttt cgctgcctgc cagccagaag
    12001 acaaagcctc tttgaaaagc atgttgcgta acaatatcgc catcatcacc tcctataacg
    12061 acatgctctc cgcgcaccag ccttatgaac actatccaga aatcattcgt aaagccctgc
    12121 atgaagcgaa tgcggttggt caggttgcgg gcggtgttcc ggcgatgtgt gatggtgtca
    12181 cccaggggca ggatggaatg gaattgtcgc tgctaagccg cgaagtgata gcgatgtctg
    12241 cggcggtggg gctgtcccat aacatgtttg atggtgctct gttcctcggt gtgtgcgaca
    12301 agattgtccc gggtctgacg atggcagccc tgtcgtttgg tcatttgcct gcggtgtttg
    12361 tgccgtctgg accgatggca agcggtttgc caaataaaga aaaagtgcgt attcgccagc
    12421 tttatgccga aggtaaagtg gaccgcatgg ccttactgga gtcagaagcc gcgtcttacc
    12481 atgcgccggg aacatgtact ttctacggta ctgccaacac caaccagatg gtggtggaat
    12541 ttatggggat gcaattgcca ggctcttctt ttgttcatcc ggattctccg ctgcgcgatg
    12601 ctttgaccgc tgccgctgcg cgtcaggtta cacgcatgac cggtaatggt aatgaatgga
    12661 tgccgatcgg taagatgatc gatgagaaag tggtggtgaa cggtatcgtt gcactgttgg
    12721 cgaccggtgg ttccactaac cacaccatgc acctggtggc gatggcgcgc gcggccggta
    12781 ttcagattaa ctgggatgac ttctctgacc tttctgatgt tgtaccgctg atggcacgtc
    12841 tctacccgaa cggtccggcc gatattaacc acttccaggc ggcaggtggc gtaccggttc
    12901 tggtgcgtga actgctcaaa gcaggcctgc tgcatgaaga tgtcaatacg gtggcaggct
    12961 ttggtctgtt tcgttatacc cttgaaccat ggctgaataa tggtgaactg gactggcggg
    13021 aaggggcgga aaaatcactc gacagcaatg tgatcgcttc cttcgaacaa cctttctctc
    13081 atcatggtgg gacaaaagtg ttaagcggta acctgggccg tgcggttatg aaaacctctg
    13141 ccgtgccggt tgagaaccag gtgattgaag cgccagcggt tgtttttgaa agccagcatg
    13201 acgttatgcc ggcctttgaa gcgggtttgc tggaccgcga ttgtgtcgtt gttgtccgtc
    13261 atcaggggcc aaaagcgaac ggaatgccag aattacataa actcatgccg ccacttggtg
    13321 tattattgga ccggtgtttc aaaattgcgt tagttaccga tggacgactc tccggcgctt
    13381 caggtaaagt gccgtcagct atccacgtaa caccagaagc ctacgatggc gggctgctgg
    13441 caaaagtgcg cgacggggac atcattcgtg tgaatggaca gacaggcgaa ctgacactgc
    13501 tggtagacga agcggaactg gctgctcgcg aaccgcacat tcctgacctg agcgcgtcac
    13561 gcgtgagaac aggacgtgaa ttattcagcg ccttgcgtga aaaactgtcc ggtgccgaac
    13621 agggcgcaac ctgtatcact ttttaagacg acaaatttgt aatcaggcga gagaaaactc
    13681 tgatgaaaaa ctggaaaaca agtgcagaat caatcctgac caccggcccg gttgtaccgg
    13741 ttatcgtggt aaaaaaactg gaacacgcgg tgccgatggc aaaagcgttg gttgctggtg
    13801 gggtgcgcgt tctggaagtg actctgcgta ccgagtgtgc agttgacgct atccgtgcta
    13861 tcgccaaaga agtgcctgaa gcgattgtgg gtgccggtac ggtgctgaat ccacagcagc
    13921 tggcagaagt cactgaagcg ggtgcacagt tcgcaattag cccgggtctg accgagccgc
    13981 tgctgaaagc tgctaccgaa gggactattc ctctgattcc ggggatcagc actgtttccg
    14041 aactgatgct gggtatggac tacggtttga aagagttcaa attcttcccg gctgaagcta
    14101 acggcggcgt gaaagccctg caggcgatcg cgggtccgtt ctcccaggtc cgtttctgcc
    14161 cgacgggtgg tatttctccg gctaactacc gtgactacct ggcactgaaa agcgtgctgt
    14221 gcatcggtgg ttcctggctg gttccggcag atgcgctgga agcgggcgat tacgaccgca
    14281 ttactaagct ggcgcgtgaa gctgtagaag gcgctaagct gtaaaagtca aaatgcccga
    14341 tctaggatcg ggcatttttg tagcgttttt tgccgggttt aaccctgtac ttttacctgt
    14401 ccggcggcgt gcttcgcgcg ttcaatggcg tcaacaacac tctctgaagt agccagtacc
    14461 acccccagac gacggctgcc atcaatttcc ggcttaccaa ataaacgaat ctgcaaatct
    14521 gcgcctacgg cattctgcac attatcaaac gtgacattct gactggtcag ttgtggcaga
    14581 ataacggcag aagctgcagg accatactga cggatcccgc caacaggaag tccgaggaag
    14641 gcacgtacat gcagggcaaa ctctgagaga tcttgagaaa ttaacgtcac catcccggta
    14701 tcatgtggac gaggggagac ctcactgaaa atcacctcat caccacagac aaacagctcg
    14761 acaccaaaca gaccataacc gcccagtgcc agcaccactt tacgggcaat ctcctgcgca
    14821 cgttcaaggg caagcgggct catttgctgt ggttgccagg attcacggta gtcgccatct
    14881 tcctggcgat gacctactgg tgcacagaaa tggacgccat ccaccgcgct gacggttagc
    14941 agggtaattt cgaagtcaaa cttaacgacg ccttcaacaa ttacgcggcc cgctccggcg
    15001 cgaccgcctt gctgagcgta ttcccatgcc tgagcaagtt gctctgcaga gcgaataaac
    15061 gtctgcccct tgccggaaga gctcatcacc ggttttacaa tgcagggata gccaatggcg
    15121 gcaaccgcct cgcggaaaag gcttttgcta tcggcaaaac gataagtgga agtgggcagc
    15181 tgcagctctt ctgccgccag gcgacggata ccctcgcgat tcatcgttaa tttcgttgcg
    15241 cgagcgcagg ggacaacatt cagtccttcc tcttcaagtt ggatcagcat atcggtggca
    15301 atagcttcga tctccggcac gatataatgt ggtttttcca gttcaaccac acggcgtaat
    15361 gcatcaccat caagcatatt aatgacatgg gagcgatgcg cgacatgcat ggctggtgcg
    15421 tcggcatagc gatcgacggc aatcacctct acgccgagac gctgacactc gattgccact
    15481 tctttaccca gttcaccgga gcctaataac atcacgcgag ttgctgccgg acgcagcgca
    15541 gtgcctaata acgtcataac gaatatcccc tgattaattc cgcgcgcagt ataaacgaaa
    15601 acgtttgcgt gtgtctttat tgctgatgtt gatttctcaa ccgaaaagaa atatactgta
    15661 taaaatcaca gttattatga gaggctgcaa atggcggttg aagttaaata cgtagtcatt
    15721 cgtgagggcg aagagaaaat gtcgtttacc agcaaaaagg aagccgatgc ttatgacaaa
    15781 atgcttgata cggcggatct gcttgacacc tagctgacaa attctccagt gcaaatggaa
    15841 gacgagcaac gtgaagccct ttcgctatgg ctggcagaac aaaaagatgt gctgagcacc
    15901 attctgaaaa ccggcaaatt accgtcccca ctggtcgtcg gtgctgaatc cgaagaggaa
    15961 gatgcctcac acgcggcctg aagcaacttg cgctcctgac aatttgtacc atgctttctc
    16021 ctgaatcatt gtgctatgga gaaaaacatg aaaaaaagag gggcgttttt agggcggttg
    16081 ttggtttctg cctgcgcatc agttttcgct gccaataatg aaaccagcaa gtcggtcact
    16141 ttcccaaagt gtgaaggtct ggatgctacc ggaattgccg cgagcgtaaa acgtgattat
    16201 caacaaaatc gcgtggcgcg ctgggcagat gatcaaaaaa ttgtcggtca ggccgatccc
    16261 gtggcctggg tcagtttgca ggacattcag ggtaaagatg ataaatggtc agtaccgcta
    16321 accgtgcgtg gtaaaagtgc cgatattcat taccaggtca gcgtggactg caaagcggga
    16381 atggcggaat atcagcggcg ttaatggctt ctgataattc gattgaccgt tggcggatgc
    16441 ggcgcgagcg ccttgtccgt cccagaacgc accacaatcg taggcctgag aagacgcaac
    16501 agcgtcgcat cgggcaatca gcatcaaaca atgccactca aaacgacaaa ctactttcac
    16561 ctaaaatacc cttatcacct gatatacagt aaatacctta cctggcgcga atttctttga
    16621 ataacggcct accgccccgc ttatttgcgc atcttttgtc tcgatataca ttttctgaca
    16681 tcctcatccg ctatgctaca ggcactaaaa atggttattg aggagcaaat atggctaact
    16741 ggttaaatca actgcaatcc ctgcttgggc aaagcagttc ttctacctcc tcgtctgcgg
    16801 atcagggatt gggcaaactg ttagtgccag gcgcattagg cggactggct gggctgctgg
    16861 tcgcaaataa atcagcacgt aaacttctta cgaaatatgg caccaacgcg ttactggttg
    16921 gcggcggagc ggtagcgggt acggtgctgt ggaataaata caaagataaa attcgcgcgg
    16981 cgcatcagga cgaaccgcag tttggcgcgc aaagtacgcc gctggatgag cgtacggaac
    17041 gtttgatcct tgcgctggtc tttgccgcta aaagtgatgg tcatattgat gccaatgaac
    17101 gtgcggcaat cgaccagcaa ttgcgtgaag ccggcgtgga agagcagggg cgtgtactca
    17161 ttgagcaggc aatcgaacaa ccgctggatc cacaacgcct ggctaccggg gtccgcaatg
    17221 aagaagaggc gctggaaatc tatttcctga gctgcgcggc tattgatatt gaccatttta
    17281 tggaacgcag ttacctgaat gcactgggtg acgcgctgaa aatcccccag gatgttcgtg
    17341 aaggcattga acgcgatctc gaacagcaaa aacgcacttt ggcggaataa ttccgctctc
    17401 tggatagcct gaagttgact atttcgggct atccagaacc gcacagccat ttgaacaggc
    17461 tgattttctc tgtctgcata acattcgata tccgcgccat tacctgtcat catctaagca
    17521 atgacgaccc tgtttcgctt gcatccccgg tgagttttgc cacccttata agatgtttca
    17581 accagaaaga acaataacat gctaccaaaa gccgcccgca ttccccacgc catgacgctt
    17641 catggcgata cgcgcatcga taattactac tggctgcggg acgatacgcg ttctcagcca
    17701 gaagtcctgg actacctgca acaagaaaat agttacggtc atcgggtgat ggcctcacaa
    17761 caagccttgc aggatcgcat cttaaaggaa atcatcgacc gcattccgca aagagaagtt
    17821 tctgcgccct acataaaaaa tggctaccgc tatcggcata tttatgaacc aggctgtgaa
    17881 tatgctatct accagcgtca atcggcattc agcgaagagt gggatgagtg ggaaacattg
    17941 ctcgatgcca acaagcgcgc agctcatagt gagttttatt cgatgggcgg aatggcgatt
    18001 acgcccgata acaccattat ggcgctggca gaagattttc tttcccgacg ccagtacggc
    18061 attcgttttc gtaatctgga aacaggtaac tggtacccgg aactgctgga taacgttgaa
    18121 cccagctttg tctgggcaaa tgactcctgg actttctact atgttcgcaa gcatccggtg
    18181 acgctgctgc cttatcaggt ctggcgtcac gccatcggta cgccagcatc gcaagataca
    18241 ctgatctacg aagaaaaaga cgatacctat tatgtcagcc tgcataaaac gacgtcgaag
    18301 cactatgtcg tcattcatct ggccagcgcc accaccagtg aagttcgcct gctggacgcg
    18361 gaaatggccg atgccgagcc gtttgttttt ctgccgcgcc gcaaagatca cgaatacagc
    18421 cttgatcact accagcatcg tttttatctg cgttccaatc gccacggcaa aaactttggc
    18481 ttataccgta cccgtatgcg tgatgagcaa cagtgggaag agttaattcc gccacgtgaa
    18541 aacatcatgc tggaagggtt tacgctgttt accgactggc tggtggttga agagcgtcag
    18601 cgcgggttaa ccagtttgcg ccaaattaac cgcaagaccc gggaagtcat tggcattgcc
    18661 tttgatgatc cggcctatgt gacctggatt gcctacaatc cagaacctga aaccgcgcga
    18721 ttgcgttatg gttattcttc catgaccaca ccagacactt tgtttgaact ggatatggat
    18781 accggtgagc gtcgtgtatt aaaacaaaca gaagtgcccg gttttgatgc ggcgaattac
    18841 cgcagtgaac acctgtggat agtcgcccgt gatggcgtcg aagttccggt ttcgctggtc
    18901 tatcatcgca aacattttcg caaaggacac aacccgttgc tggtgtatgg ctatggttct
    18961 tacggcgcaa gtattgatgc cgatttcagt tttagccgct tgagtttgtt agatcgtggc
    19021 tttgtctacg ccattgtcca tgttcgcggc ggtggtgagc tggggcaaca atggtacgaa
    19081 gacggtaaat ttctgaagaa gaaaaatacg tttaatgatt atcttgatgc ctgcgatgca
    19141 ttgttaaaac tgggctatgg ctctccttcg ctctgttatg cgatgggcgg aagtgcgggg
    19201 ggcatgttga tgggcgttgc aattaatcaa cgcccggaat tattccacgg cgttatcgcc
    19261 caggtaccgt ttgttgatgt tgtaacaacg atgcttgatg aatcaattcc tcttaccact
    19321 ggtgagtttg aagagtgggg aaatccgcag gatccgcaat attacgagta catgaaaagc
    19381 tacagcccgt atgacaacgt caccgcacag gcttatccgc atttactggt aacgaccggt
    19441 ttacacgatt ctcaggtgca acattgggaa ccggcaaaat gggtcgctaa attgcgcgag
    19501 ctgaaaaccg atgaccatct tttattgctc tgtaccgaca tggactcagg ccatggcggt
    19561 aaatctggcc gctttaaatc gtacgaaggc gtagcgatgg aatatgcttt tctggtcgcg
    19621 ctggcgcagg gaacattacc cactacgcct gcggattaag tattttccag ataatgtttc
    19681 agtgttaaac gcagctccgg gctcatgctg tccaggttat taaataacca gcgcagatag
    19741 cccggatcgc gttcggcaac gtcggaaacc gctttgccac ggtatttgcc aaaggtgaag
    19801 gtcgtcatca acgacggacg tccggtgata tcggccatct gttctgccgt ccagccggag
    19861 gtgttcataa tatcgataag caacgcggcg gtgatataac agtcatacaa cgcgcggtga
    19921 tgatgcaggc ccggcggtgt ctgtacattg agcttgcgtg ttttatataa cgccatattg
    19981 ctgtacttga gccctggcca caaacgacgg gccagtttca tagtgcaaat ccactcaccg
    20041 ggcatctcag gcagtacgcg gcggtcaaag ctggcgttgt gcgcgacata ccattcacta
    20101 ccgtaatagt gtgggatcac atcttcaatc cacggtttat cggcgaccat ggcttcggtg
    20161 atgcgatgaa tcgccatcgc ttgtggacta ataggacgat cggggcgcac caggaggctc
    20221 atggggttga cgatttttcc gtcaatgaca tcaacagagg caatctcaac gatccctccc
    20281 tgcaaaccgc aggtttctgt atcgataatg cgcaacatgg catgctccag gccgaaaaag
    20341 cctagcgtaa tggaatgata tcgccttgcc aaccctgtga tgaacgctga ccgactaaca
    20401 ataatgaacc acgatcggcg cgaacgatga gacgaccata ttcatcccat agtgcactgt
    20461 taccacgggc atttgccatc agcacggcaa tcgagtactg atgtgaaaaa aattgtaagc
    20521 gacgggccga cgccagcagg tcgggttctc ccagacattg ccctgtcgta aacagtgaac
    20581 aggtcgggtc catatccata ccttgcggtt gttcatcgac caccgtgatg gttctggaac
    20641 gacggcccag acatgcacca tgactctgat ggtagttccc aggcgttttt cgccacggtg
    20701 cgaacaccgc aattccacga ataaagcgat cgttatattc aacgggaagg ccggcaatga
    20761 tggtcattcg ccaggtcgtt gcggcataac aaagcgggtc taacagtgaa agatcgggtg
    20821 gggcaggaag ggcacgtcgc gaataatcac accccagtaa agaaagcgat ggaaaaacta
    20881 acagctgaca ttgttgcctg gcggcggccc tcacaaactc cagatgatga gctacctgtt
    20941 cggtgagtga ggttttacga ggctcatatt gcgctgctgc aactttccag aacgacataa
    21001 tgacttcctt ttcaaaatga ctgcaaatta tgggggcggt ttagaaaata ctcataattt
    21061 agcaggcgtt atgtaagaaa gtgtaactct gtataagttt tatttaagtt tgggctcgta
    21121 aggtaaacgt gacagattga ccgaagccaa acggtgggca ataagccgct cgcgaaacca
    21181 gctgcgcaaa tgttctggct gttcacgttc aaccgcttca gcgatcaccg gcatattgta
    21241 gcgttcttta aaagccaccc cggccgccgc caaatcgaca ttcactttat ccatttctgt
    21301 ttgatccagt ttagccagat tcttcagcat tcttaatctc ctcatcattc gcgggcaaaa
    21361 gttactaaga gtgagcctgg atggcaagcc ggaattgatg atcctcgact cagacattag
    21421 acaaggttat gctctgtaaa aaagttatta caaaaaggaa tagataatat ggcttcaact
    21481 gcacgctccc ttcgctacgc gctgacaatc ctgaccactt cgttggtgac accttccgtc
    21541 tgggcacatg cgcatttaac gcatcagtat cctgcggcaa acgcgcaagt gacagctgcc
    21601 ccgcaggcaa tcactttaaa cttctcggaa ggcgttgaaa caggattcag tggtgcaaaa
    21661 atcacggggc caaaaaacga aaatattaaa acattaccag cgaagcggaa tgagcaggac
    21721 caaaagcaac tgattgttcc actggccgat tcgctgaaac ccggaaccta caccgttgac
    21781 tggcatgttg tttcggtgga cgggcataaa acgaaaggac actacacctt tagcgtgaaa
    21841 taaatcatgc tggcgtttac ctggatcgcg ctgcgattta tccatttcac ctcgttgatg
    21901 ctggtctttg gctttgcaat gtatggtgcc tggctggccc ccttaatgat tcgtcgcttg
    21961 ctgacgaagc gttctctgcg tttacaacag cacgctgccg tctggagttt gatcagtgcc
    22021 actgcaatgc tcgccgttca gggcgggttg atgggaacgg gatggacaga tgtattttcg
    22081 ccaaacattt ggcaggctgt tttgcaaacg cagtttggtg gcgtctggtt atggcaaatt
    22141 gttctcgccc tcgtaacgtt gatcgtcgcc cttatgcaac cgcggaatat gccacgtttg
    22201 ttgtttatgc tcactaccgc gcaatttatt ctgctggcgg gagtggggca tgcgacgctg
    22261 aatgaagggg taacagcgaa aatccatcag actaatcacg cgattcacct gatttgtgcg
    22321 gcggcgtggt ttgggggatt actgccggtg ctctggtgta tgcaactcat caaaggccgt
    22381 tggcgacacc aggctattca ggcgctgatg cgtttttcct ggtgcgggca ttttgcggtg
    22441 atcggcgtac tggcaagcgg cgtgcttaat gcattgttga ttaccggatt tccccccacg
    22501 cttacgactt actagggcca actcttattg ctcaaagcca tcctggtgat gattatggtg
    22561 gtcattgcgc tggctaatcg ttatgttctt gtaccgcgca tgaggcagga tgaagatcgt
    22621 gcggcaccct ggtttgtgtg gatgactaaa ctcgaatggg cgataggtgc ggtagtgctg
    22681 gtgatcatca gcctgcttgc gactctcgaa cctttttgat ggattacgaa aatgatgaaa
    22741 aaaagtattc tggcgtttct gttactcacc agttctgcag cggcgctggc ggctccacag
    22801 gtgataaccg tgagtcgttt tgaagtgggt aaagacaaat gggcgtttaa tcgcgaagag
    22861 gtaatgctga cttgccgacc gggtaatgct ttgtatgtca tcaacccaag taccctcgtg
    22921 cagtatcctt taaacgatat cgcacaaaag gaagttgcca gtgggaagac caatgcccaa
    22981 cccatttcgg tgattcagat tgatgatcct aacaatcccg gcgaaaaaat gagtctggca
    23041 ccgtttatag aacgagctga aaaactctgt taattaccta aaatagcctt ttgatttcca
    23101 ataaaaaaaa cgcctcagtt ctttcaccag aacgggcggt ttttaacatt taagctgatg
    23161 accaccgcgc tttttattga ccattttgca cgcaaactgg aaaacctggc gtcgtcatct
    23221 attcttaaag ggcaaggcaa ctaagcctgc attaatgcca acttttagcg cacggctctc
    23281 tcccaagagc catttccctg gaccgaatac aggaatcgta ttcggtcttt ttttaattgt
    23341 atttaaaatc aattagttgc aaacgtctcc ccgaaattcc ccgaaattta ctcgaatttc
    23401 tgtattccgg tcttttttgg ttatatcaca accaaaatac atttaacaat ccatttacgt
    23461 taaaatcaga gcagtaagta cgttttttct ctctcatcaa gatacatttt tgttgtcttc
    23521 tccgatgtgt ggccaagtag acgctgagca aattcttctc cacatatttc tttgtacaat
    23581 cgtccagcca ggcttctgat ttcgtgaaaa gttggtgggt tttcactgaa caggatacct
    23641 gttaattttc tggctgcgac aaatttcttt gtcaggccgt ccgggtgaat gctgccgtca
    23701 gggctgtttt ttctaatccc ggcactgatt agataatccc ccctacttac catgcggcac
    23761 tgttcaacta ctgcaccaag tcgtaaacca gcgacaggaa ggctgagtga cagggggata
    23821 gcaatcatca ttcctgtctt aatttgcctg atgtggagac gatcatcata aatatcactg
    23881 aaccgcatat tcgttatgtc ttcgcgacgt tgtcctgtta caagggctaa gtccatagct
    23941 aatgggaacc atgccggaag ttgatctgct gcctccctga tgcagttgta tgtctttagt
    24001 ttcagccgtt ctcttgtaac tactattttc ggtgctcttg ttggcgttac tggattttga
    24061 gatatacgt
//
LOCUS       DADMEQ010000056        23693 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.056, whole genome shotgun sequence.
ACCESSION   DADMEQ010000056 DADMEQ010000000
VERSION     DADMEQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.056"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            580..2508
                     /gene="thrS"
                     /locus_tag="JNZ05_002237"
     CDS             580..2508
                     /gene="thrS"
                     /locus_tag="JNZ05_002237"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAY8565458.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKIPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2512..3054
                     /gene="infC"
                     /locus_tag="JNZ05_002238"
     CDS             2512..3054
                     /gene="infC"
                     /locus_tag="JNZ05_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAY8565459.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3151..3348
                     /gene="rpmI"
                     /locus_tag="JNZ05_002239"
     CDS             3151..3348
                     /gene="rpmI"
                     /locus_tag="JNZ05_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAY8565460.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3401..3757
                     /gene="rplT"
                     /locus_tag="JNZ05_002240"
     CDS             3401..3757
                     /gene="rplT"
                     /locus_tag="JNZ05_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAY8565461.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            3880..3924
                     /locus_tag="JNZ05_002241"
     CDS             3880..3924
                     /locus_tag="JNZ05_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl--tRNA ligase operon leader peptide"
                     /protein_id="HAY8565462.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4207..5190
                     /gene="pheS"
                     /locus_tag="JNZ05_002242"
     CDS             4207..5190
                     /gene="pheS"
                     /locus_tag="JNZ05_002242"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAY8565463.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5205..7592
                     /gene="pheT"
                     /locus_tag="JNZ05_002243"
     CDS             5205..7592
                     /gene="pheT"
                     /locus_tag="JNZ05_002243"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAY8565464.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEATEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7597..7896
                     /gene="ihfA"
                     /locus_tag="JNZ05_002244"
     CDS             7597..7896
                     /gene="ihfA"
                     /locus_tag="JNZ05_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HAY8565465.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7997..8977
                     /gene="btuC"
                     /locus_tag="JNZ05_002245"
     CDS             7997..8977
                     /gene="btuC"
                     /locus_tag="JNZ05_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HAY8565466.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9040..9591
                     /gene="btuE"
                     /locus_tag="JNZ05_002246"
     CDS             9040..9591
                     /gene="btuE"
                     /locus_tag="JNZ05_002246"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HAY8565467.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYQDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9591..10340
                     /gene="btuD"
                     /locus_tag="JNZ05_002247"
     CDS             9591..10340
                     /gene="btuD"
                     /locus_tag="JNZ05_002247"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HAY8565468.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10418..10882
                     /locus_tag="JNZ05_002248"
     CDS             10418..10882
                     /locus_tag="JNZ05_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY8565469.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11129..11831
                     /gene="ydiV"
                     /locus_tag="JNZ05_002249"
                     /pseudo
     CDS             11129..11831
                     /gene="ydiV"
                     /locus_tag="JNZ05_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
     gene            11895..13331
                     /locus_tag="JNZ05_002250"
     CDS             11895..13331
                     /locus_tag="JNZ05_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HAY8565470.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMPMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="JNZ05_002251"
     CDS             complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="JNZ05_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HAY8565471.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="JNZ05_002252"
     CDS             complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="JNZ05_002252"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HAY8565472.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="JNZ05_002253"
     CDS             complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="JNZ05_002253"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HAY8565473.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16026..18404
                     /gene="ppsA"
                     /locus_tag="JNZ05_002254"
     CDS             16026..18404
                     /gene="ppsA"
                     /locus_tag="JNZ05_002254"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HAY8565474.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18461..20161)
                     /gene="fadK"
                     /locus_tag="JNZ05_002255"
                     /pseudo
     CDS             complement(18461..20161)
                     /gene="fadK"
                     /locus_tag="JNZ05_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707412.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(20164..20457)
                     /locus_tag="JNZ05_002256"
     CDS             complement(20164..20457)
                     /locus_tag="JNZ05_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY8565475.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20454..21743)
                     /locus_tag="JNZ05_002257"
     CDS             complement(20454..21743)
                     /locus_tag="JNZ05_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY8565476.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21769..22737)
                     /locus_tag="JNZ05_002258"
     CDS             complement(21769..22737)
                     /locus_tag="JNZ05_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HAY8565477.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSRQFPFQPHLPL"
     gene            complement(22757..23521)
                     /locus_tag="JNZ05_002259"
     CDS             complement(22757..23521)
                     /locus_tag="JNZ05_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HAY8565478.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5843 a         5781 c         6247 g         5822 t
ORIGIN      
        1 tgcaatctat gaggagactt ataatgagtt ctgataatga tatcgttttg actcataatc
       61 tattcgcctt atatatatct ggaggttttg aggatgatga ttaattatta tcgccagaac
      121 gaaatgtgca cgaaaaattg ttataagaat tgtgtcactt ttatcattgt gatatgacca
      181 cttcgtcatt aatctcatga gcgtttaaaa tactaccgcc aaatcacgca acgaactttg
      241 ctggattcag tttatgctgc tgtaaatccg ctcgcagtaa acctttcaga cgcaccgtga
      301 tgttatcagt ggttctttaa gcgttttgct ggtgtactca ctacaaacga attgcgaatc
      361 gaatcaatgt gaaacggaaa ggtacaatct cccttttttt agtgttaaca tcgttcaacc
      421 gggtggaagg tgagaggtta atgtctttca tttgtgtacc tgattgcgaa ccaatttagc
      481 atttgttggc tagatggttt cgcaatgaac tgttaataaa caaatttttc tttgtatgtg
      541 atctttcgtg tgggtcacca ctgcaaataa ggatataaaa tgcctgttat aactcttcct
      601 gatggcagcc aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt
      661 ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct
      721 tgcgatctga ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt
      781 ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg
      841 ccgcatacca aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat
      901 cttgaccgca cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt
      961 gctgagaaaa actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact
     1021 ttcgccaacc gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat
     1081 gacaagccag gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta
     1141 ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt
     1201 ggcgacagca acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa
     1261 gcacttaacg cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc
     1321 ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac
     1381 aacgacggct ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag
     1441 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
     1501 ggtcactggg acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac
     1561 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct
     1621 tatcgcgatc tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca
     1681 ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc
     1741 tgtactgaag aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg
     1801 tacagcactt ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt
     1861 attggcagcg acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa
     1921 aacaacatcc cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa
     1981 tttaccctgt atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc
     2041 tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taagataccg
     2101 gtaatgattc accgcgcaat tctggggtcg atggagcgtt tcatcggtat cctgaccgaa
     2161 gaattcgctg gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt
     2221 accgattcac agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt
     2281 cgtgttaaag cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg
     2341 cgtcgcgtcc catatatgct ggtctgtggt gataaagagg tggaatcagg caaagttgcc
     2401 gttcgcaccc gccgtggtaa agacctggga agcatggacg taaatgaagt gatcgagaag
     2461 ctgcaacaag agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc
     2521 ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa
     2581 gttcgcttaa caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg
     2641 gagaaagcag aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg
     2701 gtttgtcgta taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa
     2761 cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tagtacagat
     2821 gaaggcgact atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa
     2881 gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa
     2941 gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag tagtcgaatc cttcccaacg
     3001 aagatcgaag gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt
     3061 caagtagcac tatctgtgga gcctttgggc ttcacaggca ttgttcgcct ttgtttcgtt
     3121 tattaacaat gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct
     3181 gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt
     3241 cacattctga ctaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt
     3301 tccaaaggcg atctgggctt ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt
     3361 tttaactttt taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg
     3421 tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc
     3481 gcgttctcgc gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta
     3541 ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc
     3601 agcagcacgt cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc
     3661 tgttgaaatc gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac
     3721 cgctctggtt gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt
     3781 ctccctcttt tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa
     3841 taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc
     3901 ttcttttttt actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa
     3961 acagcgcctg aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa
     4021 agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac acggccagtg cggagaaacc
     4081 ggagcgtact taagtacgtg aggatttcga gcactgcccg ggaccaaaat ggcaagtaaa
     4141 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
     4201 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
     4261 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
     4321 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
     4381 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
     4441 gaaagcgctg cactgaatgc gcgtctggcg gcggaaacga ttgatgtctc tctgccaggt
     4501 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
     4561 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
     4621 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
     4681 tggtttgacg ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
     4741 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gtgtttatcg taacgactac
     4801 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
     4861 agctttacca acctgaaagg cacgctgcac gacttcctgc gtaacttctt tgaggaagat
     4921 ttgcagattc gcttccgtcc ttcctacttc ccgtttaccg agccttctgc agaagttgac
     4981 gtcatgggta aaaacggtaa atggctggaa gtgctgggct gcgggatggt gcatccgaac
     5041 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
     5101 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
     5161 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
     5221 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tacgctggcg aatcaaatca
     5281 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
     5341 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
     5401 aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg cggtgcgcca aactgccgtc
     5461 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
     5521 aagcggcgaa actgcgtggc gaaccgtctg aagggatgct gtgctccttt tctgagctgg
     5581 gaatttctga cgatcacagt ggcattatcg aactgcctgc ggatgcgccg attggcaccg
     5641 acatccgcga atacctgaaa ctcgatgata acacgatcga aatcagcgtg acaccaaacc
     5701 gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc
     5761 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta
     5821 cagtcgaagc gccggaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg
     5881 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
     5941 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
     6001 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatggcaaaa gagggcgaaa
     6061 cgctggtgct gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
     6121 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
     6181 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
     6241 gtgctcgtcg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
     6301 cggtactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg
     6361 gtgaggctgg cccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
     6421 tcactctacg tcgtagcaaa ctggatcgcc tgataggcca tcatattgct gatgagcagg
     6481 taactgacat tctgcgtcgt ctcggctgcg aagcgaccga aggcaaagac gaatggcagg
     6541 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtc gaagaagtcg
     6601 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
     6661 gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
     6721 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
     6781 caggcgttga agccttactg ctggcaagcc cgatctctgt tgaaatgtca gcaatgcgtc
     6841 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
     6901 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
     6961 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
     7021 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgaccttgaa tccgttctcg
     7081 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
     7141 cggggcaatc cgcagcgatt tatctgaaag gtgaacgtat tggttttgtt ggagttgttc
     7201 atcctgaact ggaacgtaaa ctagatctta acggtcgcac tctggtgttc gaactggagt
     7261 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
     7321 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
     7381 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
     7441 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
     7501 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
     7561 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
     7621 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
     7681 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
     7741 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
     7801 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
     7861 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
     7921 atgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
     7981 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
     8041 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
     8101 acaatggatt tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat
     8161 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
     8221 tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
     8281 cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg
     8341 ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
     8401 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
     8461 ctgcagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
     8521 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
     8581 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtct aggccgatga atatgttagc
     8641 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
     8701 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
     8761 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
     8821 acttcccggc tgcgcgctgg caggggcgag cgcattgctg ctggccgata ttgtagcgcg
     8881 cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
     8941 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
     9001 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
     9061 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
     9121 ctgttaattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
     9181 aatattcaaa aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
     9241 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
     9301 ggggtgacgt tcccgatgtt cagtaagatt gaagttaatg gcgaaggacg ccatccgctg
     9361 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
     9421 gcccgtatgg tcagcaaagg ccgtgcaccg ctgtaccagg atgatatttt atggaatttt
     9481 gaaaaattcc tagttggcag ggacggaaaa gtcatccagc gtttttcccc ggatatgacg
     9541 ccggaagatc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
     9601 tgatgcagtt acaggatgtt gcggaatcta cccgcctggg gccgctttca ggcgaggttc
     9661 gggctgggga aatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg
     9721 cgcgaatggc cggaatgacc agcggtaagg gaagcattca gttcgcgggg caaccactgg
     9781 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
     9841 cgccgtttgc aatgccggtc tggcactacc tgacactgca tcagcacgat aaaacgcgta
     9901 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggtcgtagca
     9961 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgttgcaaa
    10021 tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc
    10081 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgcgctgtgt cagcaaggac
    10141 tggcgattgt gatgagcagt cacgatctca accacacgtt gcgtcatgcg catcgggcgt
    10201 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    10261 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    10321 tgctgatttc gaccatctga tttcattagt atcccgaaaa tcgggtaatt acgacgctaa
    10381 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    10441 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    10501 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    10561 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    10621 gacgatgcgc gatcgtttcg atttgcatct gccccgtgaa accaaacaac aagcctctat
    10681 tggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    10741 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    10801 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    10861 ctggcaggcg agacgaatct agtgtgtcga aaaattgacg gcgatgatac atcgccgtca
    10921 atttactcct gaatacagta gtattttgca gacaaaaatt ccgtaaatgg attattcgtt
    10981 tataatcagg ataatctaaa aaaaggcccc ctgaaaaagt ggttaacatg gaacaaatga
    11041 aagttactcc ttctgtaatt gttatgtctt atggcctcat cccttttagc cggatactga
    11101 aaaacatcct tcgagaggga cggttaccat gaagattttt ttggagaatc tttatcattc
    11161 tgattgctat ttcctgccga taagagataa ccagcaactc cttgtcggcg ttgagttaat
    11221 tacccatttc tccagtgaag atggcacggt tcgtatcccg acgagtcggg ttatagcgca
    11281 acttaccgag gagcaacatt ggcaattatt ttcagagcag ttggaattac taaaatcatg
    11341 ccaacacttt tttattcaac ataaactctt tgcctggctt aatttaacgc cacaagtggc
    11401 tacattatta ttagacaggg ataattacgc aggcgaacta ttaaaatatc cgtttattga
    11461 gctgttgatt aatgagaatt acccgcatct caatgaagga aaagataatc gcgatctttt
    11521 gtccttatcg caaatgtatc cccttgtgtt aggcaatttg ggagcaggta atagcacaat
    11581 gaaagcggtt tttgatggtc ttttcacccg cgtaatgctg gagaaaggaa caaatcacgc
    11641 atcgctcctt cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt
    11701 gcatcatcgc ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt
    11761 tccatgctct ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg
    11821 ttcagcgata accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca
    11881 aacagatagg atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa
    11941 cctatacagc actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg
    12001 aactcgctaa cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg
    12061 gcggcgaaac cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt
    12121 tcggcgtctg ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc
    12181 ttgctgatgg cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc
    12241 gaatgggtga tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg
    12301 cgatgcatta tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc
    12361 cagtgtatcg ggaaacggtg gagccaggcg cgatgccgat gcgtgtggca ccaagtcatc
    12421 tgcgctttgg tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt
    12481 tggctgactt tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc
    12541 gtctctggtt taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg
    12601 tcggctttgc acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg
    12661 attacgggcc gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg
    12721 atcatcaagg gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc
    12781 gtctggcgca gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca
    12841 gctatcagca ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca
    12901 tgacggagca aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag
    12961 agcgcagcga ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg
    13021 cgtcaccgct acgtgatgag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt
    13081 atcgggggcg tttgcaacaa gacgaggtta gcgatagtga gcgtcagcaa ctgatgcaaa
    13141 gcgttaaccc agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag
    13201 aaaagggtga tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg
    13261 acagagatga tgactatgtc agtcgtccac ctgactgggg taaacggctg gaagtcagct
    13321 gctcgagtta aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga
    13381 tattcttgcc cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg
    13441 gtaattcgcc ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaggcgtg
    13501 agttttctgc tatccgtata acgcataaaa attaataata attgatataa caatgattcc
    13561 tattatcaat attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg
    13621 cgcagaaatg cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa
    13681 aggcgagttt ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg
    13741 attgaccgta agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac
    13801 tttccgccat aattccagca atctccgtag agccattgcg gatttgctga caaatatcct
    13861 cacaaacttc taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca
    13921 ccagatgttc aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg
    13981 catgataatt cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg
    14041 tctgatagat ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg
    14101 ctgcgcggat agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta
    14161 ccggacagga gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc
    14221 caatcgcgcc ccaactgatt aaatcagcaa taaactgacc ggtcaccata tcgaggaact
    14281 cagtcgcggt agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca
    14341 gaccgtgatt tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc
    14401 cgacaacagt tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt
    14461 actggttgcg cagcgactgc agacgggtgg cgtactccat tgcagcggtg agatcgtgga
    14521 tcgagcaggg gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa
    14581 ttctgcggcg ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg
    14641 cgagttcggc gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt
    14701 tcattacaag tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca
    14761 tcacgataaa ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg
    14821 ttagcatgat aacaaaaaaa caaatcaatg cactagttct ctagtacatt cggcgactaa
    14881 ggcccatgat atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa
    14941 tccacgggat ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact
    15001 gacgaagcga ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt
    15061 ccgggtcgat agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca
    15121 ccagattatc catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca
    15181 ttgccagata cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct
    15241 gagcctgatc cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg
    15301 ccgcaatgcg cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag
    15361 caatcggcgt tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga
    15421 tatcctggca aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg
    15481 agtagaagac cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca
    15541 ctgcccgtgc acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg
    15601 gaaattgtga cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa
    15661 tataaaaaac gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata
    15721 attctatgaa agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata
    15781 aaatgaacct tcattttatt ttttatgaaa acagcatttc atttttatgg tttcgtttat
    15841 accgatggtt tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc
    15901 ttaaaaagta aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggtaaaat
    15961 atgcaaagat aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt
    16021 gttcgatgtc caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga
    16081 atgatgtaga cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt
    16141 ccggaatggg tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt
    16201 ttctggacca aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg
    16261 acgatgttac tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc
    16321 ccttccagcc tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg
    16381 acgaaaacgc ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt
    16441 cctttgccgg tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg
    16501 cagtgaaaca tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc
    16561 agggttacga tcaccgtggc gtggcgctct ccgccggtgt tcagcggatg gtgcgctccg
    16621 acctcgcatc ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg
    16681 tgtttatcac ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg
    16741 atgagtttta cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca
    16801 ccatggggtc gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg
    16861 ttaaaatcga agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag
    16921 tgcaggaact ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata
    16981 ttgagtgggc gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa
    17041 ccgtgcgctc acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta
    17101 tcgccgaagg ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg
    17161 acatcagcga aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc
    17221 cggactggga accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta
    17281 cctgtcacgc ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag
    17341 atgcaacaga acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata
    17401 ccggttacgt ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga
    17461 tgccggatct gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact
    17521 tcgcctgcct gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta
    17581 tgattggcgt ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa
    17641 acgaaatccg cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc
    17701 tgactgaagg gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc
    17761 tctctgattt taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag
    17821 atgaagagaa cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc
    17881 gcgactgctt cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga
    17941 ccaacgttga gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg
    18001 aagaactggc gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt
    18061 gtgaaatccc gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct
    18121 caattggctc aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg
    18181 tgtctgaatt gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc
    18241 gtgccgcgaa gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg
    18301 aagactttgc cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca
    18361 ccgtggtgca aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg
    18421 cgttcagtcg ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga
    18481 catcctgcgt taatcgccgt ataatatctt tccttaacaa aaacttttgt attttatctg
    18541 aggcagtgcg cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga
    18601 cccgtttacg gctaaaaaaa gccaccatct cttccagcga taatgaatga tgcggcgctt
    18661 tctgcacgac ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac
    18721 aggcatcgtg aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat
    18781 tttcgccgcc gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct
    18841 catccatacg gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg
    18901 ttaattcagg ttcatcaaaa taccccataa acacattggg gccacgcgat gtttcttcac
    18961 cttcgcaacc tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg
    19021 cggcagcgta accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg
    19081 catgcggcga actttctgtg gaaccataaa cacttaataa tttaatgctg cgctgctggc
    19141 attcgcgcgc cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg
    19201 aaaggtctgc gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc
    19261 ctagcataca ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa
    19321 tatctaacaa cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc
    19381 ccgttgcgtg accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc
    19441 gcgcgcaata agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg
    19501 gcagaccctc ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta
    19561 tcgccgtggt cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg
    19621 cgggagccag tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg
    19681 gcaggattaa atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg
    19741 cctgacactt attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca
    19801 cggaaaccgc accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag
    19861 gcagttgaaa tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca
    19921 gacagctcgc ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca
    19981 cggcaatttt gtctggcatc gcacgagcgg tctgctgtca gtaatcggcc agtgaagcat
    20041 cgccccatag cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca
    20101 ctttcatttt ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca
    20161 tgattagccg tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag
    20221 aatagtgtta ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa
    20281 atgaatgttt cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt
    20341 acggaattca ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac
    20401 atggaattta ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc
    20461 gcggttgctc ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt
    20521 cccatgatca ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc
    20581 atgatgtcgg ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc
    20641 gggatcttgc gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg
    20701 tattgcgcca gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg
    20761 gcggcagcct gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc
    20821 aggcagaagc ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc
    20881 attgccagac cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta
    20941 atcagcgggc gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt
    21001 tgcgcatggg cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg
    21061 gtatagagaa atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg
    21121 gcaccttcct cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata
    21181 acctctttaa caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag
    21241 cggccaagca ttgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca
    21301 tccccagcct gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg
    21361 cctgggataa actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga
    21421 ttacgcaata cggtataaga tgcgtgttgc ggaacatctg gttgctcgcg gtgaaaatcg
    21481 agggtaacgg cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct
    21541 accggcgctg atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc
    21601 ccgccggtca tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc
    21661 ccggctcgtg ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg
    21721 gcatcaaatt tgtcatccga catgcgttat gctcccttcc cgctgcgttt acagcgggag
    21781 atgtggttga aaggggaatt ggcggctcaa ctggctaatc agggcaggga cgactttgta
    21841 gatatcgccc accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt
    21901 gatggcgaca atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat
    21961 ccccagcgtc aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc
    22021 acgctccatc cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag
    22081 caccgccgcc agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac
    22141 gccaaccaca cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc
    22201 gcgacagaga atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg
    22261 agaggtatca ctggtgcacg gttcctgaac accgggtgca agggtaatga tcgccagcgg
    22321 gctgttgatc ttttcctgag cgaacgctaa cccgccatac atccggtgtt cggcgcaaat
    22381 gtgaccatcg acaatatcca ccgccgtggc atcgttcacc agcgccgcat tcagttgcac
    22441 acttaaccgt gctgccagag ctttaccacg tttcgtcgcg gccaacagca acatagcggg
    22501 gtgtttatct ttcagcaggg cagcaatact ttcggcgtaa ttttcagtgc gttgcagcgc
    22561 gtcgttttgc tcaagaacat aaatacattt tggaccataa ggcataactg cctgcgcctg
    22621 gtcggtattt tgtacaatgg catacacctg ttggccccat tgctgagcgc cgccaaacag
    22681 ttcagcataa cgttcaggat tatcgctaaa gacccagacg ctgtttaatt gactcatcgt
    22741 ttcttctccg taggcttcag ttcagggctt tcttcagatg ttccgccagc tcggcaatgg
    22801 cctccggcga atcgttatcg agaatgatgt gcttacgttc tgtttgcggc ggtacgcgaa
    22861 tgccgaccag ttcagcaagt ggcgcgctct ggctccagtc aatatcactt gcctgccact
    22921 gatttaccgg ttttttaccc gcgccgagaa tggctttcat cgaaggaatg cgtggcacgt
    22981 taatatcgga ggtgacgcag agcacggctg gaacagagag ttcaataact tcaacatcat
    23041 cttcaagcgt gcgttcaatc accagtgtat tgccctgacg ctgaatagca ctcactgcat
    23101 taatcaccgg aagttgcaga atttctccga ccagcaagcc aacctgctgg gcataaaggt
    23161 cgccggaacc ttcaccaaag atcagtaaat cgaagccgat cttttcaatt gctgccgcca
    23221 gcgcttttgc ggtatcgaga ggcagtgcat gttcaagttg cgcatcctgc accaaataca
    23281 ggctgtgcgg cccgcgggat agcacgtctt tgcgcacttt cgagttctgc aacaatgagc
    23341 cgccaacggt cagcgcggct atctcatcgt catctgttgc gagctggctt gcagcttcaa
    23401 tggcattgag atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc agggtgtatt
    23461 ctggagtgac aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt attattttca
    23521 tgatttctcc tgtgagtcgc atacggaacc atctccgatg gaaggtaaaa atggcctgaa
    23581 aatagtgtgc gatagcggtg cggggggttc gacggagcaa gctgttttcc ttgccaacaa
    23641 tcttgcaaaa aaaatgcgat tgctaaaaca aaagtgcaat ttgcggcaat ata
//
LOCUS       DADMEQ010000057        22319 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.057, whole genome shotgun sequence.
ACCESSION   DADMEQ010000057 DADMEQ010000000
VERSION     DADMEQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.057"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(21..692)
                     /gene="queE"
                     /locus_tag="JNZ05_002260"
     CDS             complement(21..692)
                     /gene="queE"
                     /locus_tag="JNZ05_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HAY8565479.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            831..971
                     /locus_tag="JNZ05_002261"
     CDS             831..971
                     /locus_tag="JNZ05_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565480.1"
                     /translation="MSEENKENGFNHVKIFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            985..1857
                     /locus_tag="JNZ05_002262"
     CDS             985..1857
                     /locus_tag="JNZ05_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HAY8565481.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTGLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQY
                     PANPSESESTSSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDL
                     FLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1917..3215)
                     /gene="eno"
                     /locus_tag="JNZ05_002263"
     CDS             complement(1917..3215)
                     /gene="eno"
                     /locus_tag="JNZ05_002263"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAY8565482.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(3303..4940)
                     /gene="pyrG"
                     /locus_tag="JNZ05_002264"
     CDS             complement(3303..4940)
                     /gene="pyrG"
                     /locus_tag="JNZ05_002264"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HAY8565483.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVVGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(5168..5959)
                     /gene="mazG"
                     /locus_tag="JNZ05_002265"
     CDS             complement(5168..5959)
                     /gene="mazG"
                     /locus_tag="JNZ05_002265"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HAY8565484.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(6055..8289)
                     /gene="relA"
                     /locus_tag="JNZ05_002266"
     CDS             complement(6055..8289)
                     /gene="relA"
                     /locus_tag="JNZ05_002266"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HAY8565485.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LYGS"
     gene            complement(8337..9638)
                     /gene="rlmD"
                     /locus_tag="JNZ05_002267"
     CDS             complement(8337..9638)
                     /gene="rlmD"
                     /locus_tag="JNZ05_002267"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAY8565486.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9695..12451
                     /gene="barA"
                     /locus_tag="JNZ05_002268"
     CDS             9695..12451
                     /gene="barA"
                     /locus_tag="JNZ05_002268"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HAY8565487.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12683..14023)
                     /gene="gudD"
                     /locus_tag="JNZ05_002269"
     CDS             complement(12683..14023)
                     /gene="gudD"
                     /locus_tag="JNZ05_002269"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY8565488.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(14044..15384)
                     /locus_tag="JNZ05_002270"
     CDS             complement(14044..15384)
                     /locus_tag="JNZ05_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY8565489.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFETFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15386..16738)
                     /locus_tag="JNZ05_002271"
     CDS             complement(15386..16738)
                     /locus_tag="JNZ05_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8565490.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLRSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGQFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(17172..17621)
                     /locus_tag="JNZ05_002272"
     CDS             complement(17172..17621)
                     /locus_tag="JNZ05_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAY8565491.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17639..18421)
                     /gene="truC"
                     /locus_tag="JNZ05_002273"
     CDS             complement(17639..18421)
                     /gene="truC"
                     /locus_tag="JNZ05_002273"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HAY8565492.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGERLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18421..18750)
                     /locus_tag="JNZ05_002274"
     CDS             complement(18421..18750)
                     /locus_tag="JNZ05_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HAY8565493.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19372..19917)
                     /gene="syd"
                     /locus_tag="JNZ05_002275"
     CDS             complement(19372..19917)
                     /gene="syd"
                     /locus_tag="JNZ05_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HAY8565494.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19985..20833
                     /gene="queF"
                     /locus_tag="JNZ05_002276"
     CDS             19985..20833
                     /gene="queF"
                     /locus_tag="JNZ05_002276"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HAY8565495.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20945..22309
                     /gene="ppnN"
                     /locus_tag="JNZ05_002277"
     CDS             20945..22309
                     /gene="ppnN"
                     /locus_tag="JNZ05_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HAY8565496.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5510 a         5916 c         5294 g         5599 t
ORIGIN      
        1 taacgcttat aaatgtgtaa tcaggcaata tttaaatatt tatgtgtttg catcgacaaa
       61 cgccaattac gcgcaataca ggtttcaatg cacaatcgtg tggcatcatc cttttggcta
      121 atcggctgca gtgcaatgac tcgcggttta tcatcggtca gcgttgccag tagttcatcc
      181 agtgcttcaa tatcgcgtac gcgccccact ggatgcttga tttcgttggc tcgctccagt
      241 gcctgtgaca acacttcata gccgccgcgc atgttcagct ttggcgatac ggtaacccag
      301 gtattcggtg tgcagcgtac ttcatgagtt ccactggttt cgatctggca gctaaaaccg
      361 ttcttttcga gcaggtcagt cagtggcagc aaatcatgaa tgcaaggctc accacccgta
      421 atcaccacat gccgcgcggt atatccctga cgaccaatga cagccagcaa gtcttcactg
      481 ctcgccgccc cccacttatc actctctttg gtcttcgcca gaatgctgaa aagggagact
      541 tcccgatcct caagcttttc ccaggtgtgt ttggtgtcgc accaggcaca gccaaccggg
      601 catccctgta aacgaataaa aatggcgggg acgccggtaa agtaaccctc accttgcagg
      661 gtctggaaca tctcgttaat cgggtactgc atagcattct ctgtgaagtg gataattgtt
      721 aattattgca gatcctgccg caacaatcat gtcttattaa cattctgtta catgcagatt
      781 taaagaaaaa tacgaaacat atcgttaata attaaaggga gtaacgtatt atgtcagaag
      841 aaaataaaga aaatggattt aatcatgtca aaatattcac caaaattata tttatttttt
      901 ctgtattagt ttttaatgat aacgaatcta aaattaccga tgccgccgtc aatttattta
      961 tccagattta atgagaaatt tataatgcgc tatttcatac taatgttcac tttcgtatgt
     1021 tcctttgttg cagcccaacc aactattgtc ccacaattac agcaacaagt tacgggtctt
     1081 acgagtagct taaactcaca agaaaagaaa gaactgacgc acaagttaga atctattttc
     1141 aataataccc aagtgcaaat tgctgtatta attgtttcca caaccaaaga tgagacaatt
     1201 gaacaatatg ctacaagagt ttttgacaat tggcgtttag gagatgccaa acgtaatgat
     1261 gggatactga tcgttgttgc ctggtcggat cgcactgtcc gcatccaggt aggttatggg
     1321 ctggaagaaa aggtaaccga tgctctggct ggagatatca tccgtagcaa catgataccc
     1381 gcatttaaac aacaaaaatt agctaaggga ttagagttag ctataatcgc tttgaataac
     1441 caactcactt cacaacacca atatccggct aatccttcag aaagtgaatc aacgtcttcc
     1501 actgatcatt attactttgc tattttttgg gtatttgcag tgatgttctt ccctttctgg
     1561 ttttttcatc aaggcagtaa tttttgtcgc gcatgtaaaa gtggagtctg tatttcagca
     1621 atccatcttt tagatttatt cctgttctcc gataaaactt tttccattgc tgtattttcc
     1681 ttctttttta ctttcaccat atttatggtc tttacctgtt tatgtgtact tcagaaaaga
     1741 gcatctggta gaagttatca ttcagacaac agcggttccg ctggaggctc aagtggcggc
     1801 ggatttagcg gcgggggagg ttcttctggc ggtggcggcg catccggccg ctggtaactc
     1861 caccataaaa aatgccagcc cgaaggctgg cattatcaaa tcagataaag tcagacttat
     1921 gcctggcctt tgatctcttt acgaccgttg tacggtgctt tttcgcccag agcttcttcg
     1981 atacgaatca gctggttgta tttagcaaca cggtcagaac ggctcataga accagttttg
     2041 atctggcctg cagcagtacc aacagccaga tcagcgatgg tagcatcttc agtttcacca
     2101 gaacggtgag agataactgc agtgtagcca gcatctttcg ccatcttgat tgcagccaga
     2161 gtttcggtca gagaaccgat ctggttgaat ttgatcagga tggagttagc gatacctttt
     2221 tcgatacctt ctttcaggat cttggtgttg gttacgaaca ggtcgtcacc aaccagctgg
     2281 attttgtcgc ccagaacttt ggtctggtat gcgaaaccgt cccagtcaga ttcgtccaga
     2341 ccgtcttcga tagaaacgat cgggtactgt ttggtcagtt cttccaggaa gtgagtgaat
     2401 tcttcagagg tgaacgcttt gttgccttcg ccagccagaa cgtatttacc atctttgtag
     2461 aattcagaag ctgcgcagtc catcgccaga gtgatgtctt tgcccagttc ataaccagca
     2521 gctttaacag cttcagcgat aacagccaga gcttcagcgt tggaacccag gttcggcgca
     2581 tagccacctt cgtcaccaac agcagtgttc atgcctttcg ctttcagaac ttttgccaag
     2641 tgatggaaaa cttcagaacc catgcggatg gcttctttca cagttttcgc gccaaccggc
     2701 tgaatcatga attcctggat atcaacgttg ttgtcagcgt gctcaccacc gttgatgatg
     2761 ttcatcatcg gaaccggcat ggagtatttg cccggagtgc cgttcagttc agcgatgtgc
     2821 tcgtacagcg gcataccttt agcagctgca gcagctttgg cgttagccag agatacagcc
     2881 aggattgcgt tcgcgccgaa tttggatttg ttttcggtgc cgtccaggtc gatcatgatc
     2941 ttgtcaatgc cagcctgatc tttagcatct ttgccaatca gcgcctgagc gatcgggccg
     3001 tttaccgcag caacagcttt ggttacgcct ttacccagga aacgggattt gtcgccatcg
     3061 cgcagttcca gagcttcacg ggaaccagta gaagcacctg acggagcagc tgccataccg
     3121 acgaaaccac cctccagatg tacttcggct tcaacagtcg ggttaccacg ggagtcgatg
     3181 atttcacgac cgatgatttt tacgattttg gacattaggt tttcctcaag tcactagtta
     3241 aactgaaact ccagacaaac aacgcgtacc caaggtacgc gttgccgttc taactttttt
     3301 acttacttcg cctgacgttt ctggaactcg ctggcggctt tcacaaagcc tgcaaacagc
     3361 gggtgaccat cacgtggagt agaagtaaac tccggatgga actggcaagc cacgaaccac
     3421 gggtgattcg gaacttcgat gatctcgacc aactgatcat ccccggaacg gcccacaacg
     3481 cgcagacctg catcttcaat ctgtttcaac agcatgttgt tgacttcgta acggtgacga
     3541 tgacgctcaa caattgtcgg cgcattgtat agctggcgaa ccaggctatc gtcaaccaac
     3601 tggcactgct gtgcgccgag acgcatggta ccgccgagat cgctcttctc gctacgaact
     3661 tcaacgttgc cgttttcatc gcgccactcg gtaatcagcg ccacaaccgg gtacttacag
     3721 tctggcacaa attctgtaga gttggcgttc tccatgttgg caacatggcg agcgtaatca
     3781 attaacgcca cctgcatacc caggcaaatg cccagataag gaatattgtt ctcacgcgca
     3841 aaacgcgcgg tcgtaatcat gccttctacg ccacgatagc cgaaaccgcc aggtacgagg
     3901 attgcgtcca gacctttaag gatttcaacg ccgcgcgttt caacatcttg tgaatcgatc
     3961 agtttgatgt tgacgctgac acgattcttc agcccaccgt gtttcagtgc ttcgatcact
     4021 gatttataag catccggcag ttcaatgtac ttgccgacca taccgatggt gacttcactt
     4081 accggattcg cttcttcgaa gataacctgt tcccattcgg acagatttgc ttccgggcag
     4141 tttaagctga atcgtttaca aatataatcg tccagcccct gagatttcaa caggcccgga
     4201 attttataga tggaatcgac gtctttcaga gaaataaccg ctttttccgg aacattacag
     4261 aacaatgcaa tcttcgcacg ttcgttcgcc ggtacagcgc gatctgaacg acaaatcagg
     4321 atgtcaggct ggataccgat ggagagcagc tcttttacag agtgctgagt cggtttggtt
     4381 ttgacttcac cagacgctgc catgtacggc accagcgtca ggtgcataaa cagagtgtgc
     4441 tcacggccaa tttcaacagc catctggcga atagcttcga ggaacggcaa ggattcgata
     4501 tcacctactg taccgccgat ttctaccagt actacgtcat gaccttcgcc accttccagc
     4561 acgcgctctt tgattgcgtt agtgatgtgc ggaataacct gcacggttgc gccgaggtag
     4621 tcaccgcggc gttctttacg cagaacgtca gagtagatac gacccgtggt gaagttgttg
     4681 cggcggctca ttttggtacg aatgaaacgc tcgtagtgcc ccaggtccaa gtcggtttca
     4741 gcgccgtctt cagtaacgaa cacttccccg tgttggattg ggctcatagt acctggatcg
     4801 acgttgatgt aaggatccag tttcatgatg gtcacattga ggccacgggc ttcaagaatg
     4861 gctgcgaggg aggctgcggc aatgccttta cccagagagg atacgacccc gccggtcaca
     4921 aaaatatagt tcgttgtcat gctgaacctg agaagttagg ttgaaaagac gatggaataa
     4981 ccaagacggg aaagcagtat acccgaacat gacctgtgcc acaaactttc attatccctc
     5041 ctcttcgcca gcgcactatt gaaatcagga gtgagaaaat agcccctttg gggtaaatgt
     5101 ttttgacgca aatcaagcgc ttgtcattta aaaaatgaca caaatggcgc ttgaccgcgt
     5161 aattccctta gagatcaatt tcctgccgtt ttacctgttg ccagacttct tccattgttt
     5221 cgaggtcaac acctgtcatt tccagtccac gcgcggcaac aatacgctcc acttcgcgaa
     5281 aacgacgctc gaatttttcg ttcacttttt gcaatgcgat ttctgctttc gtccctaaat
     5341 ggcgagccag attaaccgtg gcaaacagca ggtcccccat ttcctcctcc agtttagcct
     5401 ggtcgacaac agcctgccgc gcctcgtaca tcacctcgtc gatctcttcg tagactttat
     5461 caaccaccgg gccaagcgtc gtccagtcga agccaacgtt ggcgcaacgt ttctggattt
     5521 tttgcgcccg cattaaagcc ggtaaactac gaggaatatc gtccagcgcc gaatgctgcg
     5581 ctttctgcgc gcgctcttcg gttttgattt gctcccaacg ggcaagcact tcactactgt
     5641 tttcggcaga actatcagca aaaacatgcg gatggcgacg ctctaattta tcgctaatag
     5701 cagcgcaaat atcattaaag tcaaagcgcc cttcttcctg agccatttgc gcgtaaaaca
     5761 ccacctggaa tagcagatcg cccagttcac cgcgaagatc gtcaaaatct tcacgggcga
     5821 tggcgtccag cacttcgtag gtttcttcaa gggtgtaagg cgcaatggtg gcaaatgtct
     5881 gctctttatc ccacgggcag ccgttttccg gatcgcgcag gcgctgcata atagtgagca
     5941 aacggtcgat ttgattcatt gaattgtcct gaaaattgcg ggtctgtcag gtgtaatacc
     6001 ttcgagtaaa agatatcgct ggatacggct gacgccatat ccagctatgt aagactaact
     6061 cccgtacaac cgacgcgcgt cgataacatc cggcacctgg ttgagtttac ccagcacgcg
     6121 ccccagcact tgcaggttgt aaatctcaat ggtcatgtcg atggtcgcca gttgctgttt
     6181 ggtgtcgcta cggctggcaa cgccaagcac gttcaccttc tcgttggcga gaatggtcgt
     6241 gatatcacgt aacaacccac tacgatcatt agccaccacg cggaccacca gcgaatatcc
     6301 ggcggagtag ctctcacccc ataccgcgtc aacaatgcgt tctggcgcat gggagcgcag
     6361 ttccgccagt tgttcgcaat cggcgcggtg tactgaaata ccgcgtccct gggtaatgaa
     6421 gccgacaatc tcatctccag gaatcggctg gcagcagcgc gcgatgtggt gcatcaggtt
     6481 accaacacct tcaaccacca cacgaccgtt atctttactg cggttttgcg gtgtgtagct
     6541 tttttgctga agctgtttca gcgcggcggc gtcctgctct tcggcactcg gcttattaaa
     6601 ttgcgattgc aggaagttca ccatctgatt gagacggata tccccgccac caatcgccgc
     6661 cagcaactcg tcgacatcat tgaagttgta acgcggcagc agatgttttt ctgcttcttt
     6721 caggctgatc cccagatgtt ccagctcgtc gtcaaggatt tgccgcccag ccagaatgtt
     6781 tttgtcacgg tcctgtttac ggaaccaggc gtgaattttc gaacgcccac ggctggttgt
     6841 gacgtaaccg aggtttgggt ttaaccagtc acggctgggg ttcggctgtt tctgggtgat
     6901 aatttcaatc tggtcgccca tttgcagctg gtaggtgaac ggcacaatgc gcccgccaat
     6961 ttttgccccg atgcagcggt gtccgacatc actgtggatg tggtaagcga agtccagcgg
     7021 cgttgatccc gcaggcaaat caacgacatc acctttcggc gtaaagacat acacccggtc
     7081 gtcaaacacc tgactacgta cttcgtcgag catttcgccg gaatcagcca tctcttcctg
     7141 ccacgcaatc agtttacgca gccaggcaat ccggtcttca tgtcccgaac gtgcgccgcc
     7201 agctgccgcg ccctctttat atttccagtg cgcagcaaca cccaactccg catcttcatg
     7261 catctgtttg gtgcggattt ggatctcaac ggtttttcca cccggcccca gaaccacggt
     7321 atgaatagac tgataaccgt ttggtttcgg gttagcgacg taatcgtcaa actcatccgg
     7381 caggtggcga tagtgagtgt gcactatccc cagtgcggca taacaatcct gtaaacgctc
     7441 ggcgacaata cgtaccgcac gcacatcaaa cagctcatcg aaggcgaggt tctttttctg
     7501 cattttgcgc cagatgctgt agatgtgttt cggacgacca tacacttcag ctttaacgcc
     7561 ttcagctttc atctcagcgc gcagatggcc gacaaactct tcgatatagt gttcgcggtc
     7621 gagacggcgt tcatgcagca gttttgcgat gcgtttgtat tcggtcgggt ggagataacg
     7681 gaagcagtaa tcttccagct cccatttcag ttgcccaatc ccaagacggt tcgccaacgg
     7741 cgcgtagata ttggtgcact cttttgccgc cagtacacgt tcatcttccg gcgcatcttt
     7801 tacttcgcgc agatgagcaa tacgctccgc cagtttgatg actacgcagc gaaaatcatc
     7861 gaccatcgcc aataacatcc ggcgaacgtt atcgacctgt tcggaggaaa cagaatcagt
     7921 gtgcgtcgct ttcagctggc ggatcgccgc catatcacgc acgccgtgaa taaggttaac
     7981 aaccgactta ccgacgctct cacgcagcac atcttcgctg actacgttgg catcagccag
     8041 agggaacagc agcgccgccc gcagcgtgtc aatgtccata cttaatgtcg agaggatctc
     8101 caccatctca acaccacgcc acaataacag actggcatcc ggatgcccct gcgtctgttg
     8161 cagacaatac gcccaggttt cggctaagca ctcacacgac ttctggctgg taatacccag
     8221 acttgcgatc catttttccg gatcaaattc accagcctta ttgatatgtg cacttcttac
     8281 cgcaaccatc gtcctctcct ttagggacca gacctgccga aatcggcaaa tcgtaactat
     8341 ttaacgcgcg agaaaagtac catcgattcc agatgtcccg tgtgtgggaa catatccagc
     8401 atcgccagtc gcgcaatggt atatcctgct tttaataacg cttcgctatc ccgagccagc
     8461 gttgcagggt tacaggatac ataaactata cgaataggtt ccagttttat aatttgctgc
     8521 ataacacctg cggcacctgc tcgcgccggg tccagcaaca ctttatcgaa gccgtttttc
     8581 gcccacggct gctttgtgac atcttcttca agattttcgt gataaaacgt cacattctgt
     8641 aagccattaa gacgcgcatt ctgctggcct ttttccacca gcgccggaac accttctaca
     8701 ccgaccacac tggcagcttg tgtcgccaat ggcagtgtaa agttgcccat accgcagaac
     8761 agatccagta cgcgatcttc aggttgcaca tccagccatt ccaacgcacg cgctaccatt
     8821 ttttggttca cacccgcatt gacctgaata aaatcgcgcg ggctaaaagt taagcgcaac
     8881 ccgtttgagt cataccaggg catctcacca gagacggttt cgagtatctc actatcgggg
     8941 gcgagataca gatccaggcc ttcagaatgc gaaaagcgtt ccagtttttc gcgatctgcc
     9001 gaacttagcg gtgcggtatg gcgcaaaatc atcagcgtgc cgctggttgc ctgtaccagt
     9061 tcaacatgac caagatggcg catagcttgt aagctgccca gacatgccct gactttgggc
     9121 agcaatgctt caagttgggg cgctaaaatg gggcattgtt tgacgtcgac aatgtcactg
     9181 gagcccgctt tgcgaaaccc catctgaagt tgctgtgttt tcggtaagta gttcagactt
     9241 aaacgcgcgc ggcggcgata gccccaggga acatcggcga tcacttcaga gacatcgtgt
     9301 ttcattaatc gggcgagtgc cgcacttttg cttcgctgct gtaaatccac gctggcgtgt
     9361 tgttgctgac agccgccgca tacgccaaaa tgaggacagc gtggcgtttc gcgttccggg
     9421 ctatcgctta accggcgtac gactttagcg cgggcatact gttttttatc ttcagtaaca
     9481 gcaacttccg cgttttcctg cggcaataat ccggggataa ataacgtttt gccgttatgt
     9541 cgcgccacgc cctgaccaaa agagtcgagg tcgttgactg aaacggttat gatctgacgc
     9601 gtcgtcgtgc gtcgttttgc agagtagaat tgcgccattg gcgagacttt ctcaatttaa
     9661 cagtgtgacc ttaattgtcc cataacggaa ctccatgacc aactacagcc tgcgcgcacg
     9721 catgatgatt ctgatcctgg caccgaccgt ccttattggt ttattgctga gtatcttttt
     9781 cgtcgtgcat cgctataacg acttgcagcg tcaactggaa gatgccggtg ctagcattat
     9841 tgagccgctt gcagtttcta ctgaatatgg catgagcctg caaaatcgcg aatctatcgg
     9901 tcagttaata agcgtactgc atcgtcgcca ttccgatatt gttcgcgcga tttcggttta
     9961 tgatgaaaat aaccgactct ttgtcacctc caattttcat cttgatccct catcaatgca
    10021 gctcggcagc aacgtgccgt ttcctcgcca gctcactgtc actcgtgacg gcgatattat
    10081 gatcctccgc acgccgatta tttctgagag ttactccccc gacgaatcgc ccagtagcga
    10141 tgccaaaaat agtcaaaata tgttgggata tattgcgctt gagctggatc ttaaatcggt
    10201 tcgcttgcag caatataaag agatctttat ttcctgcgtg atgatgctgt tttgtatcgg
    10261 tattgcgctt atttttggct ggcgcttaat gcgcgatgta accggtccga ttcgcaacat
    10321 ggtgaatacc gtcgaccgca tccgtcgcgg gcaactcgac agccgggtgg aaggatttat
    10381 gctcggcgag ctggatatgc tgaaaaacgg tatcaactcg atggcaatgt cgctggctgc
    10441 ttatcacgaa gagatgcagc acaatatcga ccaggcgacg tccgatctgc gtgaaacgct
    10501 ggagcagatg gaaattcaga acgttgagtt ggatctggcg aaaaagcgcg cccaggaagc
    10561 ggcgcgtatt aaatccgagt ttctggcaaa tatgtcacac gagctgcgta caccactgaa
    10621 tggtgttatt ggctttaccc gcctgacgct gaaaacagaa ttaacaccaa cgcagcgcga
    10681 tcacctgaat acgattgaac gttcggcaaa taatttgctg gcaattatta atgatgttct
    10741 cgacttctcg aaactggaag caggtaagct gattctggaa agtattccat tcccactacg
    10801 cagcacgctg gatgaagtcg ttactctgct ggcacattct tctcacgata aagggttgga
    10861 actgacgctc aatattaaaa gcgacgtgcc tgataacgtg atcggcgacc cactgcgatt
    10921 acagcaaatc atcactaacc tagtggggaa tgcaattaaa ttcaccgaga atggcaacat
    10981 tgatattctg gtagaaaaac gtgcgctgag taataccaaa gtgcagattg aagtgcagat
    11041 tcgggatacc ggcattggta ttcctgaacg cgatcaatcg cgcttattcc aggccttccg
    11101 acaggctgat gccagtattt cccgccgtca tggtggcacc gggctggggc tggtgattac
    11161 acaaaaactg gttaatgaaa tgggcggcga tatttcgttc catagccagc cgaatcgcgg
    11221 ttcaactttc tggttccaca ttaatctcga tctgaacccg aacattatta tcgaagggcc
    11281 atccatccag tgcctcgcag gtaagcgcct ggcctatgtc gaaccaaact ccgcagcagc
    11341 gcaatgcacg ctggatattt taagtgaaac gccgctggaa gtggtttata gcccaacgtt
    11401 ctccgcgctg cctcccgcgc attacgacat gatgttgtta ggcatcgcgg tgaccttccg
    11461 cgagccgcta acaatgcaac atgagcgatt agcgaaagcg gtatcgatga ccgatttcct
    11521 gatgctggca cttccttgcc atgcacaagt caatgctgaa aaactcaagc aagatggtat
    11581 cggcgcgtgt ctgctgaaac cattaacacc tacgcgcctg ttgcctgccc tgacggaatt
    11641 ttgtcatcac aaacaaaaca cgcttttgcc tgtaaccgat gaaagtaagc tggcaatgac
    11701 agtcatggcg gttgatgaca accccgctaa cctgaaactt atcggcgcat tgctggaaga
    11761 tatggtgcaa catgtggaac tttgcgatag cgggcatcag gcagttgaac gggcgaaaca
    11821 gatgccgttc gatttgatct taatggatat tcaaatgccg gacatggatg gcattcgggc
    11881 ctgcgaactc atccaccaac tcccgcatca gcgacaaacg ccggttatcg cggtaacggc
    11941 gcatgcaatg gccgggcaaa aagagaagct gcttggcgca gggatgagcg attatctggc
    12001 gaaaccgatt gaagaagagc gattgcataa tttgttgttg cgctacaagc ctggcagcgg
    12061 tatttcgtct cgcgtcgtga cgcccgaagt caacgaaatt gtggtgaacc cgaatgcgac
    12121 cctcgactgg caactggcac tacgccaggc agcaggaaaa accgatttag cgcgcgatat
    12181 gctgcaaatg ttactcgatt tcctgcctga agttcgcaac aaagttgagg aacagctggt
    12241 tggagaaaac ccggaaggcc tggttgattt gattcataaa ctgcatggca gttgcggcta
    12301 tagcggcgta ccacgtatga agaatctctg ccagctgatc gaacaacagc tacgtagcgg
    12361 tactaaagaa gaagatttgg agccggagct gctggaactg ttggacgaga tggataatgt
    12421 cgcgcgcgaa gccagcaaaa ttctcgggta acggacgtct tgtcgagtag caatcggctt
    12481 tttatgacgc tggtttcaga ttggtgacaa agtgcctgtg tttatgccgg atgcggcgtt
    12541 aacgccttat ccggcctaca aaaccaatta aattcaatga attaaaaaat gatgtagacc
    12601 tgatacatag cacaacaggc tattttgcgt ttagcatcag tctcaaaccg gctccagata
    12661 gagccggttt tggttttctg tcttaacgca ccatgcacgg gcgcttgtta tcgaacgtcc
    12721 agccaggaat cagatactgc attcccatcg catcgtcacg cgcgccaagc ccgtgtttct
    12781 gatacagctc atgggctttc atcacttgat ccatatcgat ttctacaccc agccccggtt
    12841 tttctggcac ctgtaccagc ccgcctttga tctcaaacgg ttctttggtc aggcgctgat
    12901 taccttcctg ccagatccag tgcgtatcaa tagcagtaat tttacccggt gcagcggcgg
    12961 caacatgggt aaacatcgcc agggaaatat cgaagtggtt gtttgagtgt gaaccccagg
    13021 tcaggccaaa ttcatggcac atttgcgcca cacgtaccga accttgcata gtccagaaat
    13081 gcggatccgc cagcgggata tcaacggatt gtaacgagag cgtatggccc atctgccgcc
    13141 agtcggtggc gatcatattg gttgcagtcg gcaggccagt cgcgcgacgg aactctgcca
    13201 tcacttcacg cccggagaaa ccttgctccg caccacacgg atcttctgca taagccagcg
    13261 aacctttcag gtatttacca attttaattg cttcgttcag cgaccaggca ccgttaggat
    13321 cgagcgtaat acgcgcctgc gggaagcgtt gcgccagtgc cacaatagac tcggcttctt
    13381 cttccccggc cagtacaccg cctttcagtt tgaaatcgtt gaagccatat ttttcatacg
    13441 ccgcttccgc caggcgcacc accgcatccg gcgtcatcgc ttcttcatga cgcaggcgat
    13501 accagtcgca tgagtcatcc ggctggcttt gatacggcag aggtgtggct ttgcgattac
    13561 cgacgaagaa cagataaccg agcatttcga cttcgctacg ctgttgacca tcgcccagca
    13621 gcgatgccac gtttaccccc agatgctgcc ccagcagatc cagcattgcc gcttctatcc
    13681 cggtaactac atgaatagtg gtacgcaaat caaatgtctg caaaccgcgc ccactagcat
    13741 cacgatcggc aaaagtatta cgcaccagcg tcagaacgtt tttgtattca cccagcgttt
    13801 tacctaccac cagcggaatc gcatcttcca gcgttttacg gattttctcg ccgccgggaa
    13861 tttcccctac gccagtgtga ccggaattat ctttgataat cacaatatta cgcgtgaaga
    13921 acggtgcgtg tgcaccactc agattcatca gcatactgtc atgacccgcc accgggatga
    13981 cctgcatttc agtaacaaca ggcgtcgtaa attgagaact catatttatg tcctttttca
    14041 gaatcaatga cggccgaaaa cgggacgttt acggtcaaag gtccagccgg ggatcaggta
    14101 ctgcatcgga cctgcgtcgt tacgcgcacc gcaaggcaga cgtttatagg cctcatgtgc
    14161 cttttgtacc tgttcccagt ccagttccac gcccagaccg ggcgcatcag gaacggcaat
    14221 ttttccgttt ttaatctcca gcggattttt ggtcaggcga caatcgccct cctgccaaat
    14281 ccagtgggta tcgatagcgg taggattacc cggtgccgcc gcgcccacat gggtaaatat
    14341 cgccagagag atatcgaaat ggttgttgga atggcagccc caggtcagcc cccagtcgtc
    14401 gcaaagctgc gccacgcgga ctgcaccgga aagcgtccag aagtgcggat cggcaagtgg
    14461 aatatctacc gcattgagca tcaccgcatg acccatttcg cgccagttgg tggcgatcat
    14521 gttagtcgcg acgggcaagc cggtcgcccg tcgaaactcc gccatcactt cacgtccgga
    14581 gaagccctgt tctgcgccgc atggatcttc ggcataggta agaacatcat tcagcccttt
    14641 gcataaagaa atggcttcat caagcagcca tgcaccgttg ggatcaacgg taatccgcgc
    14701 atccgggaag cttttcttca atgcacgaac agtgtcgatt tcttgctcgc caggtaacac
    14761 cccgccctta agtttgaaat ctttaaagcc atagcgatcc tgtgaggctt ccgccagacg
    14821 cacaacggct tcgctgttca tcgctttctg atggcgcaac tgataccact catggttgcc
    14881 cggcgtattt tccagataag gaagatcggt tttggtccga tcacctatat aaaacagata
    14941 accgaggacg gtaatagtct cgcgttgctt gcctggcccc aacagttcgc agaccggaac
    15001 attcagcgcc ttacctagca ggtcaagcaa ggcggcttcc agcgccgcca cggcattaac
    15061 gcgcaattca aaagtccagg cacctttgcc gaaggtctca aaatcggctg cctgattacc
    15121 tttatgtacc tgctgaacca ctttattcag gcgcgcaact tcctggccca gaaccatcgg
    15181 aatagcatcg accagtgttt gataaatcac ctctccgccc ggcgcttcac caatgccggt
    15241 atgcccggcg ttatcggtga gtaccacgat attgcgggtg aagtatgcgt tatgcgcacc
    15301 accaatattg agcaacatgc tgtcatgtcc ggcgaccgga atgactttca tatcagtaat
    15361 aacaggactg gattgtgtcg ccatcttatt gccccgcgac aggtttcagc tcgatacgct
    15421 tgatatcgcc caccagcacc aggtagctca gtaccgcgat tagggcatga acaccaacat
    15481 aaatcagcgc cccattaaac gagccagtcg tgccaacgat ataaccaatt gcgattggcg
    15541 tgacgatgcc agaaatgtta ccgaacatgt tgaactggcc accggaaaga ccgctgatct
    15601 cttttggcgc ggtatctgcc attactgccc aacccagcgc cccgatgccc ttaccgaaga
    15661 aggccagcgc cataaagccg atgatcatcc actcaacgtt gacgtagttg cagaacacca
    15721 tcaccatcga cagcaacatg cccattacga tcggtgtttt acgcgcaatg ttcagcgatc
    15781 ccgtgcggcg catcagccag tcggaaataa ttccacccag cacaccgccg ataaaaccgc
    15841 aaaccgccgg aacggaagcc acaaagcccg ctttcagaat cgacatccca cgcgcctgca
    15901 ccagataaac cggaaaccag gtaatgaaaa agtaagtcag ggcgttgata cagtactgac
    15961 cgatataaac gccgatcatc atccgtgacc tgagcagctg tttgatctgc ccccacttca
    16021 cgctgaacgg cactttaact ttggtgcttt gctgatccat attgatcagc gcgccacccg
    16081 cggcgatata ctccagctct ttctgattta cccccggatg ttgatttggc tcgtggatga
    16141 ctttcaacca gataaagctg atgacaatgc ccagaccgcc cataaagaag aagacgtgtg
    16201 accagcccac ttcatgcgtc agccagccca taatcggcgc gaagatcacc gttgcgaagt
    16261 attgagcgga gttaaaaatc gacaccgccg ttcccctttc ctgcgccgga aaccaggccg
    16321 caacaatgcg actgttgccg gggaaagatg gcgcttcagc aagcccgacc aggaagcgca
    16381 gagtaaacag ggcaacgata atgccgaatc cactaaagat atcgacgaag ccttgcagca
    16441 aggtaaacat cgaccagata aagatcgacc agaagtagac gcgttttgaa ccaaaacgat
    16501 ccagcaacca accaccaggg atctgcccga taacataagc ccatgagaag gcagagaaca
    16561 catagcccat tcccacggga tcaaggccga tatctttggc catttccgaa ccggcaatag
    16621 aaagcgtagc gcggtcgccg tagttgaagg atgtgacgat aaacaacatc accactatcc
    16681 agtaacgagc attcgtgcgt ttttccacac tgctcgcagc ctgacttaaa gaactcattg
    16741 ttgcactcct gaaaattcgc gttagccacg ctcactctgg actgcgacat cgccaggaaa
    16801 tcagaggtga cgtagggtgt tttttgccgt ttttataggt cgttcgccga atacggcgcg
    16861 tgtttatatc tggcaatagc agtataaaaa gcgcgccata gcggctcacc gtgcaacaac
    16921 acaacattaa tgcgttcaat gaggcccgat tttggcatta gccctggacg gtggaatcca
    16981 cttcacggaa atgaaaacaa gaacaagaaa ggaagggtaa aaacgaagaa ataaagaaga
    17041 gtatgaaatg gatcgcttga ctctaggcaa acgccagtaa aaatccgcgc tatgaagcag
    17101 tttttactgg catttgcctg aaaagattcg attcagcacc gctaaaacga catttaccgc
    17161 tcgctgacat atcaggacaa cagcgtgccc cactgttcga cccacggatt tgattccgtt
    17221 tccggttccg ggttctcact ggcgtcaatc aacagcattt cgccaacccg ctgagcgctc
    17281 tgttcctgca acaaggcatc gaactgtttg ccgccattgc aaaaatttac ataactgctg
    17341 tcgccgagcg caatcacgcc ataacgcaga ttcggctgga agcccagact atctttgatt
    17401 ccctgaaaga gcggcacaat gctatcagga aggtcgccct gcccggtcgt ggacgtaacc
    17461 accagaacat acttatcctg atagggcagc cagtcgctta attcaggatc ttcaaatacc
    17521 gttgctttgt ggccctgtgc ggtcagaatt gcttctgctt cttccgccac taacagcgaa
    17581 tttccgtaca tggtgccgac aaaaatacca atttccgcca taccgttttc tccctggatt
    17641 aggaacttat ctctccatcc tgacccgatg gcgcactgaa ctcaaccctt tcattttcag
    17701 gaagcagacc gcgccagcca aattgtgata acgcctgcat ccaggtgtcg tccaggcccg
    17761 cgtgaatagt cagccgctcg ccagtaaaag gatgcgtcaa tgacaactga ctggcatgta
    17821 gcattaaccg ctggaggcca aaatgctcag caccgctgcg attctggcgt aaatctccat
    17881 gtttgctatc gccaataatc ggatgacgca aatgggcaag atgtcggcga agctgatgtt
    17941 tgcgtccggt tttcggctcc agttccacca ggccgtagcg cgtggtcggg taacgtccgg
    18001 tcgctaccgg catttctacg gtcgccagac cgcgataatg cgtcactgct ggctgcgggc
    18061 ctttatcttc gcgggcaaat ttatcagcga ttttgtccag ttcttccacc agtggataat
    18121 ccaacaccgc ttcttccatc aaccagccgc gcacaatcgc atggtaacgt ttctggattt
    18181 ggtgctgttc aaactgttgt gccagcagcc gtccggcctc gctggataat cccatcaaca
    18241 acacaccaga agtgggtcgg tccagacgat gagcagtaaa aacatgctgt cctatctggt
    18301 cacgcacggt ttgcatgacc actactttct cgtcgcgatc cagccagcta cggtgaacca
    18361 gccagccgga gggtttattt accgcaacca gccattcatc ctgatagagt atttccagca
    18421 ttagctcgca tcatccgcaa aaagagcatc cagtttttcc aactcagcca gaataagcgc
    18481 gcgttgcgga tggtccgtcg ctagcgccat ttcataatag ggtgcaacag caaaagcgcc
    18541 cggtaacggc tggttgttat ttaacaaatc gtgcattcgc gggatcagcc cccactgcaa
    18601 ccactccagt ggttccatgg tgtccataaa gaacggttgg gtactattaa attgatgcgg
    18661 ctggggttca tcgtttcgcc agtgctgatg ttcacgcagt aacgcttcaa gcgcctgcaa
    18721 ctggagacga acgcggtcat gagtggtcat aaagcaacct caataagaaa aactgccgcg
    18781 aaggatagca ggaataaaaa aagggagcac tgtattcaca gtgctcccgg ttcgtttcgc
    18841 agcattccag ctacttttgt tgctccctgc tcatccttga caacttttcc tctggccttg
    18901 cggccaatcg ttcatcctga actattgctt cctgctcaca ccaccccgat gtgatacttc
    18961 atcctgaagt gtccctggcc ttcctgaccc accgaatcat cctgaccggt tctcattctc
    19021 catcctggag gtgtccttta acgcgtcctg cgtcatcctc ttcgcttcat ccagaagcct
    19081 ttccctgaaa caccatcctg gtgtgtcctg cagaagtgtc atcatcctga tgttcacttc
    19141 gttgtctgac tccctgtcga cgaagataga atcgtctttt tcgcgaagtc ttacaaggcg
    19201 cttacagaca atgccttaag gaaaatttca tatgaaagtt aagaattaac aatcaacatt
    19261 ttgattttaa atgaaaataa cgattcttat ctttttaagt attcgcttat ttcctggcga
    19321 tctcctacag agtctgtaag agatctctca caaccagatg gggtatttag attacagaag
    19381 aggcttaagt tgattaagga attccgcaag atttgaggct aaatgcgtcc gtttacgtgt
    19441 tcccagcgtc tctttgcaca cttcaccgga gagattacac accgagatga cctctaactc
    19501 ttcttccagc gtcgcgataa atagcgtggg cggcagtttt agccttttct gagtcaccag
    19561 atgaccaatc agattttcct gcacccgacg gaagtcgtct tcactccagg tttgcagcaa
    19621 tgtcagtttg atatcgccaa actgcgcgtg catatccccg gcaaactgag tcgtataaaa
    19681 ggtatgaatt gtgggttgta tcacaatatc aaaagcgcgt tcaaccgcgt ttacattttg
    19741 ttccccggta aacggctggg gttgccagta tacggcatct tcggtagttg aaataatgca
    19801 cggtgaagga acaccgtaca gttcctcact taacggccag cttttatgct cttcatgcca
    19861 cgcatcgcag tagcgtgcag taaaatcttt cagggcttgt gcggtcaaat cgtccactaa
    19921 tttctctctt cacttaaacc agatacactt gtcgtttagt ttatctggtt tatgacggtg
    19981 aaacatgtct tcttatgcaa accatcaggc acttgcgggc ctgactcttg gaaaatcaac
    20041 cgattaccgg gatacctatg acgccagcct actgcaaggc gttccacgca gcctgaatcg
    20101 cgacccgctg ggtctgaaag cggataacct gccttttcac ggtacggata tctggacgct
    20161 gtatgaactt tcctggctga atgcgaaagg tttgccgcag gtcgctgtcg gtcatgttga
    20221 acttgattac accagcgtaa atctgattga gtcgaagagt tttaagctct atctcaacag
    20281 ttttaaccag acgcgtttta ataactggga tgaggtgcgc cagacgctgg agcgcgactt
    20341 aagcacttgc gctcagggta agattagcgt ggcgttatat cgtcttgatg aactggaagg
    20401 ccagccgata ggtcatttca atggcacttg cattgatgac caggatatca ctatcgataa
    20461 ctatgaattc actactgact atctggagaa tgccaccagc ggtgaaaaag tagtggaaga
    20521 gacgctcgtc agccacctgc tgaaatcaaa ctgcctgatc acccatcaac cagattgggg
    20581 ttcgatccaa attcagtatc gtggacgcca aattgacaga gaaaaactgc tgcgttacct
    20641 ggtctcattc cgtcatcaca acgagttcca cgaacagtgc gtggaacgca tctttaatga
    20701 cctgttacgc ttctgccagc cagagaaatt gagcgtttac gcacgttata cccgtcgtgg
    20761 cggtctggac attaacccgt ggcgcagtaa tagcgatttt gtcccatcga ccacaagact
    20821 ggttcggcaa taaatttttt ctcaattttg cgtgctggat tcacgcagaa ggttgtgaaa
    20881 ggtcatcagg cagggctatt gtaatcaaag ggaatgacga tattcgtccc ataaggagtt
    20941 tttcttgatt acacatatta gcccgcttgg ctccatggat atgttgtcgc agctggaagt
    21001 ggatatgctt aaacgcaccg ccagcagcga cctctatcaa ctgtttcgca actgttcact
    21061 tgccgtactg aactccggta gtttgaccga taacagcaaa gaattgctgt ctcgttttga
    21121 aaatttcgat attaacgtcc tgcgccgtga acgcggcgta aagctggaac tgattaatcc
    21181 cccggaagag gcttttgtcg atgggcgaat tattcgcgct ttgcaggcca acttgttcgc
    21241 ggttctgcga gacattctct tcgtttacgg gcaaatccat aataccgttc gttttcccaa
    21301 cctgaatctc gacaactccg tccacatcac taacctggtc ttttccatct tgcgtaacgc
    21361 tcgcgcgctg catgtgggtg aagcgccaaa tatggtggtc tgctggggcg gtcactcaat
    21421 taacgaaaac gagtatttgt atgcccgtcg cgtcgggaac cagctgggcc tgcgtgagct
    21481 gaatatctgc accggctgtg gtccaggagc gatggaagcg ccgatgaaag gtgctgcggt
    21541 cggacacgcg cagcagcgtt acaaagacag tcgttttatt ggtatgacag agccgtcgat
    21601 tatcgccgct gaaccgccta acccgctggt caacgaattg atcatcatgc cggatatcga
    21661 aaaacgtctg gaagcgtttg tccgtatcgc tcacggcatc attatcttcc ctggcggtgt
    21721 gggtacggca gaagagttgc tgtatttgct gggaatttta atgaacccgg ccaacaaaga
    21781 tcaggtttta ccattgatcc tcaccggccc gaaagagagc gccgactact tccgcgtact
    21841 ggacgagttt gtcgtacata cgctgggcga aaacgcgcgc cgccattacc gcatcatcat
    21901 tgatgacgcc gctgaagtcg ctcgtcagat gaaaaaatcg atgccgctgg tgaaagaaaa
    21961 tcgccgtgat acaggcgatg cctacagctt taactggtca atgcgcattg cgccagattt
    22021 gcaaatgccg tttgagccgt ctcacgagaa tatggctaat ctgaagcttt acccggatca
    22081 acctgttgaa gtgctggctg ccgacctgcg ccgtgcgttc tccggtattg tggcgggtaa
    22141 cgtaaaagaa gtcggtattc gcgccattga agagtttggt ccttacaaaa tcaacggcga
    22201 taaagagatt atgcgtcgta tggacgacct gctacagggt tttgttgccc agcatcgtat
    22261 gaagttgcca ggctcagcct acatcccttg ctacgaaatc tgcacgtaat ctcccctcc
//
LOCUS       DADMEQ010000058        21644 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.058, whole genome shotgun sequence.
ACCESSION   DADMEQ010000058 DADMEQ010000000
VERSION     DADMEQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.058"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..157
                     /locus_tag="JNZ05_002278"
     CDS             <1..157
                     /locus_tag="JNZ05_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY8565497.1"
                     /translation="LSIEMNKLKQARYSIGIAMSEEKYSGIIGALRGKYINCLVTNSS
                     TAELLLK"
     gene            227..1033
                     /locus_tag="JNZ05_002279"
     CDS             227..1033
                     /locus_tag="JNZ05_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY8565498.1"
                     /translation="MQTWLNLQDKIIIVTGSASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            1043..1450
                     /locus_tag="JNZ05_002280"
     CDS             1043..1450
                     /locus_tag="JNZ05_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HAY8565499.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIA
                     VKLEKLASIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            1450..1944
                     /locus_tag="JNZ05_002281"
     CDS             1450..1944
                     /locus_tag="JNZ05_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HAY8565500.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            2010..2807
                     /locus_tag="JNZ05_002282"
     CDS             2010..2807
                     /locus_tag="JNZ05_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY8565501.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            2818..3606
                     /locus_tag="JNZ05_002283"
     CDS             2818..3606
                     /locus_tag="JNZ05_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAY8565502.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            3658..4887
                     /locus_tag="JNZ05_002284"
     CDS             3658..4887
                     /locus_tag="JNZ05_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY8565503.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            5269..5958
                     /gene="pepE"
                     /locus_tag="JNZ05_002285"
     CDS             5269..5958
                     /gene="pepE"
                     /locus_tag="JNZ05_002285"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HAY8565504.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(6049..7680)
                     /locus_tag="JNZ05_002286"
     CDS             complement(6049..7680)
                     /locus_tag="JNZ05_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAY8565505.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7900..11583)
                     /gene="metH"
                     /locus_tag="JNZ05_002287"
     CDS             complement(7900..11583)
                     /gene="metH"
                     /locus_tag="JNZ05_002287"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAY8565506.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMVDYQMESLSAE
                     INFAAAKLARARADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            11783..12607
                     /gene="iclR"
                     /locus_tag="JNZ05_002288"
     CDS             11783..12607
                     /gene="iclR"
                     /locus_tag="JNZ05_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HAY8565507.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            12924..15108
                     /locus_tag="JNZ05_002289"
                     /pseudo
     CDS             12924..15108
                     /locus_tag="JNZ05_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(15077..16813)
                     /gene="aceK"
                     /locus_tag="JNZ05_002290"
     CDS             complement(15077..16813)
                     /gene="aceK"
                     /locus_tag="JNZ05_002290"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HAY8565508.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPVLMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(16997..18301)
                     /gene="aceA"
                     /locus_tag="JNZ05_002291"
     CDS             complement(16997..18301)
                     /gene="aceA"
                     /locus_tag="JNZ05_002291"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HAY8565509.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(18331..19932)
                     /gene="aceB"
                     /locus_tag="JNZ05_002292"
     CDS             complement(18331..19932)
                     /gene="aceB"
                     /locus_tag="JNZ05_002292"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HAY8565510.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGCGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(20201..21130)
                     /gene="metA"
                     /locus_tag="JNZ05_002293"
     CDS             complement(20201..21130)
                     /gene="metA"
                     /locus_tag="JNZ05_002293"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HAY8565511.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            21287..21577
                     /locus_tag="JNZ05_002294"
     CDS             21287..21577
                     /locus_tag="JNZ05_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565512.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5338 a         5547 c         5470 g         5289 t
ORIGIN      
        1 gctctctatc gaaatgaata aattaaagca ggcgcgatat tccattggca ttgccatgag
       61 tgaagaaaaa tacagcggaa ttattggtgc actgcgtgga aaatatatta attgtctggt
      121 aacgaatagc agcacagctg aactattact gaaataaaaa tcttcccgat gtttaccgta
      181 ataaaaatta cggcgggtcc ctattatcta aaatcaggag tctgttatgc aaacgtggtt
      241 aaatttgcag gataaaatca ttattgtcac cggcagcgca tccggtattg gtctggcgat
      301 tgtagaggaa ttattagcac aaggcgcgaa tgtacagatg gtcgatattc acggtggcga
      361 tggtcaatat gaaggtcata aaggctatca gttttggccg accgatattt ctagcacaaa
      421 agaggtaaat catacggtag cggaaattat ccagcgtttt ggtcgcatcg acggtctggt
      481 caataacgcc ggggtcaatt tcccgcgtct gctggtcgat gagaaagcgc ctgccgggca
      541 gtatgaactc aacgaagctg cattcgaaaa aatggtcaat atcaaccaga aaggtgtttt
      601 tctgatgtcg caggcggtgg cgcgacagat ggtcaaacaa catgatggcg tgattgtgaa
      661 tgtttcctca gaaagtgggc tggaaggctc agaaggacaa agctgttacg ccgctaccaa
      721 agccgcgctc aatagcttca cgcgctcctg gtcgaaagag ctgggtaaac acggtatccg
      781 tgtggtcggt atcgcgcccg ggattctgga aaaaaccggg ctacgcacgc cggaatatga
      841 agaggcgctg gcgtggacgc gcaatatcac cgtcgagcag ctgcgtgaag gctataccaa
      901 aaacgccatt cctattgggc gcgccggaag attagcagaa gtagctgatt ttgtttgtta
      961 tctgttgtct gaacgcgcca gctacatcac cggagtaacc actaacattg cgggcggcaa
     1021 aacgcgcggc taaggaggct ttatggtcaa cgccattttt tgtgcccacg gcaaattagc
     1081 ttgcgccatg ctggaatcgg ttcagatggt ttacggcaac gccaatgttg aagccgttga
     1141 atttgtgccc ggtgaaaacg ccggagacat tgcggtaaag ctggaaaagt tagcaagcat
     1201 tcacaatcaa gatgagtggc tgattgccgt ggatttacag tgcggcagcc cgtggaatgc
     1261 ggcagcaatg cttgcgatgc gcaatcctcg tttgcgggta atcagcggcc tttctctgcc
     1321 gctggcgctc gaactggttg ataaccagga cagcatgaat gttgatgaac tgtgtgaaca
     1381 cctgacgcaa atcgcaaagc aaacctgcgt tgtctggaaa caactggcaa cgacagagga
     1441 ggatttctga tgaacattac gctcgcccgc attgatgacc gtctgatcca cggccaggtc
     1501 actaccgtct ggtcgaaagt ggctaacgcc cagcgcatta ttatctgcaa tgacgaagtt
     1561 tataacgatg aagtgcgccg gacattattg cgtcaggctg cgccaccggg aatgaaggtt
     1621 aacgtggtca atattgaaaa agccgtcgcg gtttatcaca acccgcaata tcaggacgaa
     1681 accgtttttt atcttttcac tcgcccacag gatgccttag cgatggttcg ccagggggta
     1741 aaaataggca ccctgaatat tggcggcatg gcctggcggc ccggtaaaaa acagttaacc
     1801 aaagcggttt cattagatga tgatgatatt aacgcatttc atgagttaaa taatcttggt
     1861 gtcatactgg atttacgcgt cgtagcgtca gatccctcaa tcaatattat cgacaaaata
     1921 aacgaacagt taatcgcaaa ttaaataata gcgcctgtgt acatatgctt taacaggcag
     1981 ggatgacgac acttaaaagg tgacaaatca tggaaataag taccctacaa atcattgcta
     2041 tatttctttt ttcctgtatt gccggaatgg gcagcgtgct ggatgaattt cagactcatc
     2101 gcccgttaat tgcctgtacg gtgattggtt taattctcgg tgatttaaaa accggaataa
     2161 tactcggcgg tacgctggaa ttgatagctc tcggctggat gaacgtcggc gcggcgcaat
     2221 ctccggattc tgcactcgcc agcataatct ccgccattct ggttatcgtt ggtcagcaaa
     2281 gcatcgccac cggaatcgcc atcgcgttgc ctgtggctgc ggcaggccag gtgctgacgg
     2341 tgtttgcccg taccatcacc gtagtgttcc agcacgcggc ggataaagca gcagaagagg
     2401 cgcggtttcg cactctcgat attctgcatg tctccgcgct tggcgtgcag gcgctgcgcg
     2461 tcgccattcc ggcactgatt gtctcactgt tcgtcagcgc cgatatggtc agcaatatgt
     2521 taaacgccat tcccgaattt gtgacccgag gactacagat tgctggcggt tttatcgtgg
     2581 tggtcggtta cgccatggtg ctacgtatga tgggcgtgaa atatttgatg cctttctttt
     2641 tcctcggttt cctcgcaggt ggctacctcg atctcagcct gctggcgttc ggtggcgtcg
     2701 gcgtgatcat ggccctggtc tacatccagc taaatccaca gtggcgtaaa gctgaacctc
     2761 aactccaggc caccgcctcc accgcccttg accaacttga cgattaacgg agccatcatg
     2821 gaacagagaa aaattacacg cagcgatctg gtgagcatgt ttctgcgctc caacctgcaa
     2881 caggcgtcct ttaactttga acgtattcac gggctgggct tttgctacga catgatcccc
     2941 gccatcaagc gactttaccc attaaaagag gatcaggttg cggcgctcag gcgacacctg
     3001 gtgttcttca ataccacgcc agccgtatgt ggcccggtca tcggcgcgga aattgatgac
     3061 ggtaccatca acggcatcaa agtcggtctg atgggaccat tggcaggagt tggcgatcca
     3121 ctggtctggg gaacgctgcg cccgattacc gccgcgctcg gcgcatctct ggcactttcg
     3181 ggcaacattc tcggcccgct gctgttcttc tttattttca acgcggtgcg tctggcgatg
     3241 aagtggtatg gcctacagct cggctttcgc aaaggggtga atatcgtcag cgatatgggc
     3301 gggaatgtgc tgcaaaaact caccgaaggc gcgtcgattc tcggactgtt tgtgatgggc
     3361 gtgctggtga ccaaatggac gtcaatcaac gtaccgttgg tggtttcaca aacgcatgcc
     3421 gccgatggct ccaccgtcac catgaccgtg cagaacattc tcgaccaact ttgccctggt
     3481 ttgctggcgc tcggtctgac gctactaatg gttcgtctgc tcaacaaaaa aattaacccg
     3541 gtatggctga ttttcgccct gtttggctta gggattatcg gcaatgctct gggcttcctg
     3601 tcctgattct tcgccccggc acgactgccg aggccatcgc tcaacatgag gtggtttatg
     3661 aaaacaacag ctctgcgtct ttatggtaaa cgtgatttac gcctggaaac ctttgacctt
     3721 cctgaaatgc aggaggatga aatcctcgca acggtggtca ctgacagcct gtgcctctct
     3781 tcctggaaag aggccaatct gggtgaaaac cataaaaaag tacccgacga tgtggcgacc
     3841 aacccaatca tcatcggcca cgaattttgc ggcgatattc tggccgtggg taaaaagtgg
     3901 cagcacaaat tccagccggg tcagcgttat gtgattcagg ccaacctgca actccccgac
     3961 cgcccggatt gccccggcta ctccttcccg tgggtaggcg gcgaagcgac gcatgtggtt
     4021 attcccaacg aggtcatgga acaagattgc ctgctggcat acgacggcga aacctatttt
     4081 gaaggctcac tggttgaacc gctttcctgc gtgattggcg cgttcaacgc caactatcat
     4141 cttcaggaag gtagttataa ccacacgatg gggattcgcc cgcaagggcg cacgctgatc
     4201 ctcggcggca ccggaccaat gggactgttg gcgattgatt atgcgctgca tggaccagtt
     4261 aacccgtcgc tgctcgtcat taccgatacc gacaacgaca aattgagtta tgcgcgcaag
     4321 cactatccat cagaaccgca aacactgatt cattatctca atgccaccga tgcagcattt
     4381 gatacgttaa tggcgctgag tggcggtcac ggcttcgatg atattttcgt ctttgtgcct
     4441 aatgaaggac tggtgactct cgcctcttcc ttgctggcga cagatggttg cctgaatttc
     4501 ttcgccggac cgcaggataa acatttcagc gcgccaatta atttctacga tgtgcattat
     4561 gcatttaccc actacgtggg cacgtcaggc ggcaataccg acgacatgcg cgcggcggtc
     4621 aaactgatcg aagagaaaaa agtgcaggcc gcaaaagtgg taacacatat tcttgggctg
     4681 aatgccgcgg gcgaaaccac gcttgaattg cctgccgtcg gcggcggcaa aaagctggtg
     4741 tataccggga aatacctgcc gctgacgtca ctcacgcaga ttcaggatca agcactggcg
     4801 gcgattctgg cgcgtcatca ggggatctgg tccggtgagg cggagcaata cctgctcgcc
     4861 catgcagagg caatttccca tgattaatcg cgctacttaa ctctgcatgt cgctggctgg
     4921 tcgccccagc aacttcgcga ccggatgtgc ggctgattgc tgaagcagca caatttgcac
     4981 gagaaggata aaaaatatca gcgccttaag gggcgctcaa attggtggca aagtacacat
     5041 tgtttatgcc ggatgcggaa tgaagcctta ttcggtctac ataattttga aaatccaata
     5101 tattgcagtt atctggtagg ctaggtaagc gtagcgcatc aggcaatgct gtgtttgtca
     5161 tcagtctcag cgcccggtgc aatacgtgac atcgtgagcc atgtcagtaa gtggttaaat
     5221 tcgcagtggt agcatgcctt ccgtgatttc ttactaaccg gagaagtaat ggaactgctt
     5281 ttattgagta actcgacgct gccgggtaaa gcctggctgg aacatgcact gccgctaatt
     5341 gctgaacagt tgcagggtcg ccgctcagcg gtgtttatcc ctttcgctgg cgtaacgcag
     5401 acctgggatg attacacaga gaaaacggct gcggttctcg ctccgctggg tgtttctgtc
     5461 accggtattc atagcgttgt cgatcccgtt gccgcgattg aaaatgcgga aattgtgatt
     5521 gtcggcggcg gtaatacttt ccagttgctg aaacagtgcc gcgagcgcgg gctgctggct
     5581 ccaattactg acgtggttaa acgtggcgcg ctgtatattg gctggagcgc aggcgctaac
     5641 cttgcttgcc caaccattcg taccaccaac gatatgccga ttgtcgatcc gcaaggtttc
     5701 gatgcgctaa atctgttccc gttgcaaatc aacccgcact tcaccaacgc gctgccggaa
     5761 ggccataaag gtgaaacccg tgagcagcgt attcgcgaac tgctggtcgt cgcgccagaa
     5821 ctgacgatta ttggtctgcc ggaaggtaac tggatcacag tgagtaaagg tcacgctacg
     5881 ctgggtggcc cgaacaccac ttatgtgttt aaggctggtg aagaagcggt tccgctggaa
     5941 gctggtcacc gtttttaagt gaattgccag atgcatgatc cggcatcttg ttcatagtgc
     6001 ctgatgcgac gcttgacgcg tcttatcagg cctacaggat tcacaatatt aatactcgtc
     6061 ccgtccttca tcttcatccg gctgttccag cacactgtaa gccaccgaac aaaacagcga
     6121 gttcaggcgc tgcatatctc ccagtaagcc taaatgtagc gaactggttt caatgctctg
     6181 cacgttttgc tgatgcaggc gatcgacgtg ggcgtgggaa tagcggcgat taagaatgcg
     6241 aaaacgatgt ttgctgcgac gcaaacgacg agcgctggtg acatcgccag agaagaacac
     6301 cgacattgcc agctttaaat tactgagcaa ttgctcatag agcgcatcca gttccttcaa
     6361 cccatcaagc gaaaatgccc gccgtgctgc cagcgattta tcggcaattt cgctgcccat
     6421 gcgctcgacg atatcggagg cctgttcaag gttgagcgac atttcgatga tctccgccca
     6481 gcggcgcgac tcttcctccg ccaactcttc ttttggcatc cgcgccagat acagcttaat
     6541 ggcggtgtag agaacgttga tatcatctgc cagcttacgc agctctttct cctgccgtgg
     6601 ctcgccgtgc atcactttat tcagcccttc catcatctgt tccatggcgt cgccaatgcg
     6661 cagggtttcg cgcgcggcgt tcgccagagc aagcgtgggc gtgtccagcg cgctgacatc
     6721 cagatgttta ggccgtagct gggtatccag ttccggttca tcgcgaataa tcgttttgca
     6781 aaaccgtgcc atcgggtcaa caaatggcag catgactagg caacgtacaa ggttgtagaa
     6841 gacgtggaaa tagatcacca gttccgcttt tggcaatgac agcttcccca ttgtctctgc
     6901 cagcaaatgg acaaacggca ggataatcag gctacccacc agcttaaaca gcagactacc
     6961 cagcgcgaca cggcgggctg cggcattggc ggcactgttg ttgagcatcg ccagcaggcc
     7021 ggaaccgagg ttagctccaa tcaccagaca gagcgccacg gggaaggaga taatgcctgc
     7081 agcggtcaga gtcgcggtca gcagtacagc agcaaggctg gagtagctga taatggcgaa
     7141 catcgcgcca atcagcgcat ccagcagaat atcgccggtc agcgaggcaa agatcacctg
     7201 aacgccgttt gcctgggtga tcggcgttac ggcctgcaca atcaactcca gcgccagcaa
     7261 aatcagccca agaccaatac cgacgcggcc cagttgcccg gcgcgtgact gtttgcgtcc
     7321 gaggaaaaaa atcacgccga taaaaataag taacggtgac agccaggata agtcgaaggt
     7381 gagaatacgc gccattagcg ccgtcccgac atctgccccc agcacaatga ccagagccgg
     7441 tgcgagggct accagatcct gagcgacgaa cgaggtcacc agcatggtgg tggcattact
     7501 gctctgtacc agtgcggtaa cgccgatccc cgcgcaaaag gcgagcggct ttttttcgac
     7561 gctccggcta aggacagtac gcaaacgcgc gccgaagacg cgcattacgc cggttcgaac
     7621 aatatgagtc ccccagacca gcagggcgac ggcagaaagc aggtgaagca gcgttaacac
     7681 gagttcaggt tctccttatc gttatatgtt cctgaagcaa ttgaatgacc aaaggaccgg
     7741 aaacacgctg actggcatct ttactgtgcc ttttgacatt cgtttttccg gctaagatta
     7801 ttccagcctg tttccagtat aagtgtttca tattaataaa gagacagggc actcattgag
     7861 cgccctgttt gttgtaagga aaagtgacag atttgtgaat cagtccgcgt cataccccag
     7921 attcggtgcc agccagcgct caacatcgga gacgctcata cctttacggc gggcatagtc
     7981 ttcaacctga tcgcgctgaa tttgcgccac cgcgtagtac ttgctgtccg gatgactgaa
     8041 ataccaaccc gaaaccgatg caccaggcca catagcgaaa gattctgtga gtttcatgcc
     8101 agtatgtttt tccacttcca gcagctccca gatggtggct ttttccgtat gttccgggca
     8161 ggccggatag cccggtgccg gacggatgcc ctggtagttt tcgcggatca gttcttcgtt
     8221 gctgaggttc tcgttcggcg catagcccca gtagacttta cgcacacgct catggagata
     8281 ctccgcaaag gcttcggcta aacggtcggc aagcgctttc accatgattt tgttgtaatc
     8341 atcgtgctgc gcttcaaagg catcagccag tgcgtcctct tccagcccgc cagtcacggc
     8401 aaatgcgccg atgtaatctg ctttaccaga aagcttcggc gcaacgaagt cagcgagaca
     8461 gtagttagcg aatcccgttt tttcggtctg ttgacgcaga tggtggctga cgttgatcac
     8521 atgggtgcgc gtttcgtcac ggtagatttc aatgtcatcg cccacacggt ttgccgggaa
     8581 caggcccacc acgccacgcg gattcagcgt tttctcggcg cttaatttat ccagcatgtc
     8641 gttggcgtct ttaaacagcc gctgcgcctc aacgcccacc acttcatctt ccagaatgcg
     8701 cggatacttc ccggccagcg accaggtcat aaagaacggt gtccagtcga tgtaattacg
     8761 cagcgtttcg atgctggctt ccacttcctg cacgccgaga cggtgcgcca ccggcggcgt
     8821 gtaagcctgc cagtcaaatg cgaaatcgtt atcgcgcgcc gcttccagcg tgaccggtgg
     8881 tgtgcgtggt ttcttgcgcc cgtgctgaat acgcacggtt tcgtactcct tgcaggtacg
     8941 agcgacaaaa tcatcacgct gagtatcgga aagcagcgcc gctaccacac caacggtgcg
     9001 cgaggcattc tgcacataca ccgtagggcc gctgtagttc tgctcgattt tcaccgccgt
     9061 gtgcgctttt gaggtggtcg cgccgccaat cagtaacgga atggtgaagc cctgacgctc
     9121 catctctttc gccacgttaa ccatctcgtc cagcgacggc gtgataagcc ccgaaaggcc
     9181 aatcaaatca gcattcactt ctttggcgat acggagaatt ttttccgcag gcaccataac
     9241 gccgagatcg acaatttcgt agttgttaca ttgcagcacc acaccaacga tatttttacc
     9301 gatgtcgtgg acatcgcctt tcacggtggc gatcaccatc ttgccgttgg ttttaccctg
     9361 ctctttgctg gcttcaataa acggttcgag atacgccact gcctgtttca tgacgcgcgc
     9421 cgatttgacc acctgtggca ggaacatttt gccttcgcca aacagatcgc cgaccacgtt
     9481 catgccgtcc atcaacgggc cttcaatcac ttcaatcggg cgcgtagcct gctggcgggc
     9541 ttcttcagta tcctgctcga taaactcggt tatgcctttg accagcgagt attccagacg
     9601 tttattcacg tcccacgagc gccactccgc ttgctgggcg ttagcagtgt cgtccgcttt
     9661 actgccgcga tatttctcgg caagctccag taaacgctca gtgccatcgt cacgacgatt
     9721 aagaatcaca tcttccaccg cgtcgcgcag ttcagcgggt aggtcgtcgt aaatcgccag
     9781 ttgcccggcg ttgacgatcc ccatatccat gccattgcga atagcgtagt agaggaacac
     9841 tgcgtgaatg gcttcgcgca ccggatcgtt gccacggaat gagaaagaaa cgttagatac
     9901 gccgccggaa atcagcgcgt gcggcagttc gcgtttgatg tcttcacacg cgccgataaa
     9961 gtcctgcgcg tagttgttgt gctcttcaat accggtggcg accgcgaaga tgttcgggtc
    10021 gaagatgata tcttctggcg ggaagccaac ctcttcggtg aggattttgt acgcccgacg
    10081 gcaaatctcg attttccgtg cgcgagtatc ggcctgtccc tgttcgtcaa aggccatcac
    10141 caccactgcc gcaccgtagc gacgcaacaa tttcgcgtga tggataaagg cgtcgacgcc
    10201 ctctttcatc gagatagagt taacaatgcc tttgccctgg atacacttca gacctttttc
    10261 aatgacatcc cattttgagg agtcgatcat aatcggcacg cgagcgatat ccggttcacc
    10321 ggcaatcaga ttgagaaaac gcaccatcgc cgcttcggca tcgagcatcc cttcatccat
    10381 gttgatatcg ataatctgcg cgccgttttc cacctgctga cgcgccacat ccagcgcctc
    10441 gctgtatttc tcttctttga tcaggcgctt gaacttagca gagccggtga cgttggtgcg
    10501 ttcacccacg ttcacaaaca ggctatcgtc gccaatgttc agcggctcca ggccagacaa
    10561 acggcaggct acgggaattt ccggcagttt gcgcggcgct aatccttcta ctgcacgact
    10621 catcgctgca atatgttgcg gcgtggtccc gcagcaacca ccgacgatat tgagaaagcc
    10681 cgcttgcgcc cattcacgta tctgttttgc catcgtgtcg gcgtcgagat cgtattcacc
    10741 aaaggcgtta ggcagcccgg cgttcgggtg ggcggtgacg tagcattccg caatccgtga
    10801 tagttcctgc acgtactggc gcagttcatc gggccccagc gcacagttca ggccaaaggt
    10861 cagcgcttcg gcgtggcgca atgagttgta aaatgcttcg gtggtctgcc cggagagcgt
    10921 gcgcccggag gcgtcggtga tggtgccgga gatcataatc ggcagctcaa cgcccagcgc
    10981 ttcaaactcc gttttcaccg caaagaccgc cgctttggcg ttaagggtgt cgaaaacggt
    11041 ttcaatcagg atcagatccg caccaccttc caccagcgct ttggtggatt cgcgataggc
    11101 cgccaccagc tggtcaaaag tgatgttgcg gaatgccgga tcgttgacgt ccggagaaat
    11161 agacgccgtg cggttggttg ggccgagaac accggcaacg tagcgcggtt tctccggcgt
    11221 gcgcgcggtc cactcgtcag cacgggcgcg cgccagtttc gccgccgcaa agttgatttc
    11281 cgccgacagg gattccatct ggtaatccac catcgcaatg gtcgtggagt tgaaggtgtt
    11341 ggtttcgatg atatccgcgc ccgcttcaaa gtaggcgttg tggatagcgg cgatcacttc
    11401 cggtttactg agtaccagca ggtcgttgtt gcctttgagg tcgcatggcc agtcggcaaa
    11461 gcgttcacca cgaaaatcgg cttcgttcag tcgatagctc tggatcatgg tgcccatacc
    11521 gccgtccaac accagaatac gttcatttaa ctgcgcacgc agttgttcca ctttgctgct
    11581 cacacttgct cccgacacgc tcaacccaga tttaataaaa attcaacaaa ccatactggc
    11641 ataaacgcat ctgtggtaaa agcgaccacc acgcaacatg agatttgttc aacattaact
    11701 catcggatca gttcagcaac tattgcatta gctaacaata aaaatgaaaa taatttccat
    11761 gatacagaaa aaggaggctg tcatggtcgc acccattccc gcgaaacgcg gcagaaaacc
    11821 cgccgttgcc accgcaccag cgactggaca ggttcagtct ttaacgcgtg gcctgaaatt
    11881 actggagtgg attgccgaat ccaatggcag cgtggcactc acagagctgg cgcaacaagc
    11941 cgggttaccc aattccacga cccaccgcct gctaaccacg atgcaacagc agggtttcgt
    12001 gcgtcaggtc ggcgaactgg gacactgggc aatcggcgca catgccttta tggtcggcag
    12061 cagctttctc cagagccgta atttgttagc aattgttcac cctatcctgc gcaatctaat
    12121 ggaagagtct ggcgaaacgg tcaatatggc ggtgcttgat caaagcgatc acgaagcgat
    12181 tattatcgac caggtacagt gtacccacct gatgcgaatg tccgcgccta tcggcggtaa
    12241 attgccgatg cacgcttccg gtgcgggtaa agccttttta gcccaactga gcgaagaaca
    12301 ggtgactaag ctgctgcacc gcaaagggtt acatgcctat acccacgcaa ctctggtgtc
    12361 tcctgtgcat ttaaaagaag atctcgccca aacgcgcaaa cggggttatt catttgacga
    12421 tgaggaacat gcgctggggc tacgttgcct tgcagcgtgt attttcgatg agcaccgcga
    12481 accgtttgcc gcaatttcta tttccggacc gatttcacgt attaccgatg atcgcgtgac
    12541 cgagtttggc gcgatggtga ttaaagcggc gaaggaagtg acgctggcgt acggtggaat
    12601 gcgctgactt tttctggcgg gcagaggcaa tattctgccc atcatacctg agtggcaata
    12661 gaataagggt gtctgttaat cgcattgacg ccaaaataac ttaatgtcat acacttcaca
    12721 cacttttatt aactcagcat tatttttaaa catcaaacca cttaattatt aacaaaatcg
    12781 caagatctca ttccaccgaa acttttacgg ctgcattatg agattgagcg atttatgata
    12841 tttttttagt tgtaatcata ttaaattttt tcactgttct gatagttaaa attcaagaca
    12901 tcaataaaca atgagatatt taaatgatta ctcgtattcc tcgtagttct ttctctgcaa
    12961 atattaataa tacagcccag acaaatgaac accaaaccct gagtgaattg ttttataaag
    13021 aactcgagga taaattttct ggcaaggagc tggcgacgcc tctattaaaa agcttctcag
    13081 agaactgtag acaaaatgtt cgtcatatct ttagcaacaa ggattttgtc attaaatttt
    13141 ccacgtccgt cttacaagct gataagaaag aaattacgat aattaataaa aacgaaaaca
    13201 cgacactcac tcaaaccatt gctacaatat ttgaagaata cctaatggaa attttacctc
    13261 aacgctcaga cactcttgat aaacaagaat taaacctaaa atcagataga aaagaaaaag
    13321 aattcccaag aattaaactt aatggtcaat gttattttcc ggggcgcccc aaaaccgtat
    13381 agtataccga cacattgctg cacaatatat taatgatatt tatcagaatg ttgattacaa
    13441 accccatcaa gatgattact cttcagctga aaaatttctc actcacttca acaaaaaatg
    13501 caaaaaccag actttggcgt tgatttccag ccgtcctgag gggcgttgcg ttgctgcctg
    13561 cggtgatttc gggctagtta tgaaagcata ttttgacaag atggaatcaa atggcatcag
    13621 tgttatggca gccatattac tggtggataa ccatgctttg acggtccggc taagaataaa
    13681 gaacacaact gaaggatgta cccattacgt gatttcggtt tatgatccta atgtaactaa
    13741 cgataaaata agaattatga gcgaaagcaa agaggatatt aaacactatt ctctgatgga
    13801 ttttatgaat gtagattata gcctcctgaa atggtcaaat gatcatgtta ttaaccaatc
    13861 tgttgcaata attccagcac ttccgaaaga acagctattg atgttaaaag gaactgtgga
    13921 tgaaataacc cctccattat caccagcaac gatgaatttg ctaatggcaa ttggtcagaa
    13981 tcaccaactt acgcaactga tgattcagct ccagaaaatg ccagaactac atagaacaga
    14041 aatgttgact gcctataata gtattaacct ccccggttta tatttggcta taaattatgg
    14101 caatgcggat atcgttgaga ctattttcaa ttcattgtca gaaacaggat atgaagggtt
    14161 actctcgaaa aaaaatctca tgcatattct ggaggcaaaa gataaaaatg gtttttctgg
    14221 attattttta gcgatatcac gtaaggataa aaatgttgta acctcgattc tgaacgtctt
    14281 acctaaactg gccgcaacac atcatttaga taatgaacag gtgtataaat tcctgagtgc
    14341 caaaaataga acgtccagcc atgttttata ccatgttatg gcgaatggtg atgccgacat
    14401 gctgaaaatt gttttggtcg cgttaccttt gttaattcgc acatgtcatt tgactaaaga
    14461 acaggtactc gatctcctga aggcaaagga tttttatggt tgcccaagac tatacctggc
    14521 gatgcaaaat ggacatagct atatcgtgaa agttattctc gaagcattgc cctgtctggc
    14581 ccaggaaatt aacatttcag cttccgatat tgtagatctt ctgaccgcta aaagtcttgc
    14641 gcgcgacacg ggtttgttta tggccatgca gcgcggacat atgaacgtta ttaatactat
    14701 ttttaacgca ttacccactc tttttaatac gtttaaattc gataaaaaaa tatgaagccc
    14761 ctcctcctgg caaataattc taatgaatat cccggtttgt tttcagcgat acagcataaa
    14821 caacaaaacg ttgtagagac ggtttatctt gctttatctg accatgcacg cctgtttgga
    14881 tttaccgctg aagatattat ggatttttgg caacacaaag cgccacaaaa atactctgcc
    14941 tttgagttgg cttttgaatt tggtcaccgg gttattgctg aattaatcct taatacatta
    15001 aataagatgg ctgaaagctt tggctttacg gataaccctc gatacattgc ggagaaaaat
    15061 tatatggaag ctttactcaa aaaagcatct ccccataccg tacgctaaat ctttgcctgc
    15121 gccgatacgc ataaacatct tccacatgcc cttcacgtat gcggttttgt agtgcgcgcc
    15181 agtaatcagc gcggaacagg tcggcgtgca tctcttcaaa cagcggacca atacgcgggt
    15241 cggcgcatag ccagtggcga aactcttccg ggaaaacatc gcccggcgag acgctgtacc
    15301 acggttcgct ggcaagttcg tcttccggat agcgcggcgg cgggatgtcg cggaaattca
    15361 cttccgtcat gtagcaaatt tcatcgtaat cataaaaaac cacacgcccg tgacgggtga
    15421 caccgaagtt tttaaacagc atgtcgccag ggaaaatgtt agcagcggca agctggcgaa
    15481 tagcgttacc gtattcttca atggcgtcgc gcaactgctg accttccact tgttccagcc
    15541 agatattgag cggcaccatc cgccgctcga tatagagatg gcgaattaca atctgctcac
    15601 caagatcggt gattttttcc gctgcttcct ggagcagtaa ttccattaat accggggaaa
    15661 tatgccgctt ctccagcaca aagttttcaa actcctgggt gtccgccatt cggcccacac
    15721 ggtcgtgctc tttcaccagt tgatagcagg cacgaacgtg agcggcagac atctctttct
    15781 gcggcgcgaa cttgtctttg atgactttga atacccgatc aaagcccggc agcgtaaaca
    15841 ccaacatcac cattccacga atacccggcg cttcaatgaa ctgctcatta cagccctgta
    15901 gataaacgag atattcgcgg tagctttcgg ttttggcgtg cttctggcag ccgatagcca
    15961 tatacaattc agcagtggtt ttacctggca gaatttcccg tagccactcg accagcgctg
    16021 cgggcagcgg tgcataaacc ataaaataag aacgcgcaaa gccaaaaaca atgctcgctt
    16081 cggcggtcgt cgtcaggcag gtatcaataa ataactcgcc gtcgtccgtc tggtggatcg
    16141 gcagtaagaa gggcaacgtg ccggaaggcg tgatcagttt gcccaccagc caggcggctt
    16201 tattgcggta aaacagttcg ttcgccacct gtaaatgact ttccgcgagg ttgtctgtcc
    16261 ccagcgtttc cgtcagatgg cgaatgatgt aatgaatgtc acggctttta ttctgccagc
    16321 gcaggcgcag cggcaggtcg ctgataacgc gcatcagtag agattcccag ccgtgatcgg
    16381 ggtgaaagtc tttcgccagc gggcggggaa tggtacgaaa gcggcgctct ggctgagagc
    16441 taaagataaa aagccgctcg ggagtaagcg agcggtggtc aaataaccga cagtacacgg
    16501 agttaaaaaa gctctccgca atctcgaagc gcgggtaatc cggcaacagc cgcgtgtaat
    16561 gctctttaac gcgcagtaaa aatgccgcgt ccgtactttg gccgttagta atgcagcgca
    16621 gttgctccac gaccagacca acgtgatgat cgtaaagatg gatacggttt ttcatcgcct
    16681 gctggacagc atgccagtcg gcctgttcga aacgctgctg cgccccggaa gtcacttcga
    16741 ggaatcgacc atactgagcg tcgaagcctt gcaaaatggt ttgagcaatc agtaattcca
    16801 ggccacgcgg catttagcgc actcatcagg agcagagaat tgccggatgc ggcgtaaaca
    16861 ccttatccag cctacgttcg gcaacggttg taggcatgat aagacgcgca agcgtcgcat
    16921 caggcatcgt gcaccgattg ccggatgcgg cgtaaatgcc ttatccggcc tacagtcagc
    16981 aacgtttgta ggttgcttag aactgcgatt cttcagtgga gccagtcagc gcggtgactg
    17041 aagacgtgcc gccctgaata atagtcgtca ctttatcgaa gtaacctgta cccacttcct
    17101 gctggtgaga tacgaaggta tagccatctt tcgcggcggc aaattccggc tgctgcactt
    17161 tctcaacata gtgcttcata ccttcgccct gcgcataagc gtttgccagg tcgaacatgt
    17221 taaaccacat gctgtggata cctgccaggg tgatgaactg gaacttgtag cccatatccg
    17281 acaactgctg ctggaagctg gcaatagttt tgtcgtcgag gtttttctgc cagttgaacg
    17341 acggcgagca gttataagcc agcagtttgc ccggatattt cgcgtggata gcttgtgcaa
    17401 agcgacgcgc cagttccaga tccggcgtgg aggtttcaca ccagaccagg tcagcatatg
    17461 gcgcatacgc caggccacgg ctgatcgctt gctcaatgcc cgcatgagta cggaagaagc
    17521 cttcactggt acgctcgccg gtaataaatt cgctgtcata cgggtcgcaa tctgaggtga
    17581 tcagatccgc cgcatcagca tcggtacgtg caaccagcag ggttggaacg cccgtcacgt
    17641 cagctgccag acgcgccgcg accagtttct gaatagcttc ctgagttggc actaaaactt
    17701 tgccgcccat gtgaccgcat ttcttcactg acgccagctg atcttcgaag tgaactgccg
    17761 cagcaccggc ttcaatcatc gctttcatca gttcaaaggc attcaggaca ccgccaaaac
    17821 cggcttccgc atcggcaacg atcggcagga agtaatcgac atagcgcgga tcgcccggct
    17881 caatgcccac ggaccattgg atctgatcgg cacgacggaa ggtgttgttg atccgctcca
    17941 ccacagctgg caccgagttt gccggataga gcgactgatc cggatacatg ctggccgcca
    18001 ggttagcgtc cgccgctacc tgccatcccg acagatagac tgcttcaata cccgctttcg
    18061 cctgttgcag cgcctgaccg ccagtcagtg caccgaggct gttgatgtag ccttttttcg
    18121 actcaccgtg cagcagacgc cacattttcg ctgcgcccag ttgcgccagc gtgcattcag
    18181 gattgactga accgcgtaat ttcaccacat cttccgcact gtatgggcga gtaatgcctt
    18241 cccaacgcgg ttgagtccac tctttctgta attcttcaat ttgttgtgta cgggttttca
    18301 tgtgcagatg ctccatagtt atgtggtggt ttacgctaac aggcggtagc ctggcagggt
    18361 caggaaatca attaactcat cggaagtggt gatctgttcc atcaagcgtg cggcatcgtc
    18421 aaaacggccc tgggagaaac gttcttcgcc cagttcgctg gcaatgactt tcatctcttc
    18481 gcccagcatc tggcggaaca aggctttggt caccggtttg ccattgctca acgttttttg
    18541 atgatggatc cactgccaga tcgaggtacg ggaaatttca gccgtcgccg catcttccat
    18601 caggccataa atcggcacgc agccgttgcc ggagatccac gcttcgatgt actgcacagc
    18661 cacgcgaatg ttggcgcgca taccttcttc ggtgcgttca ccatcacaag gtgccagcag
    18721 ctgatcggca gtaatcggcg cgtcttgttc gcgcaatact tcaagctgat ttttacggga
    18781 gccgagaata tcgttgaata ccgccattgc cgtgtccgca aggcctgggt gagcgatcca
    18841 tgtgccatcg tgaccgttat tggcttccag cgctttatcc gcttttactt tgttgagcac
    18901 ctggttatta cgctcttcat ctttgctcgg aataaacgct gccatgccgc ccatcgcaaa
    18961 agcaccgcgt ttatggcagg ttttaatcag caggcgtgag taagcattca ggaatggttt
    19021 atccatcgtc acggcctgtc tgtctggcag gacacgatcg ggatagtttt tcaacgtttt
    19081 gatatagctg aagatgtaat cccagcgacc gcagttcaga ccaacaatat ggtcgcgcag
    19141 cgcgtgaagg atttcgtcca tctggaacac cgcgggcagc gtttcaatca gcaacgtcgc
    19201 cttgatggtg ccgcgcggca gattaaagcg atcttctgca tagctgaaga cttcgctcca
    19261 ccaggccgct tcctgccagg actgggtttt cggcagatag aaatagggac cgcagccctt
    19321 tgccaacagt gcctgatagt tgtggaagaa atagagcgca aaatcaaaca ggctaccggg
    19381 gattgcctca ccacgccagg tgacatgttt ttccggcaag tgcagaccgc gtacccgaca
    19441 aatcaaaacc gctggattgg gcttgagctg ataaattttg cctgcttcat tggtatagct
    19501 gatggtaccg ttaaccgcat cgcgcaggtt aatttgcccg tcgatcactt tgttccagtc
    19561 tggtgccagt gaatcttcga aatcggccat aaagactttc acattggcgt tcagtgcgtt
    19621 gatcaccatc ttgcgctcta ccgggccagt tatctctacg cggcggtctt ctaagtccgc
    19681 aggaatcccg cgaattttcc aatcagcatc gcgaatggaa gccgtttccg aaataaaatc
    19741 aggcaacgtt ccgttatcaa tatcttgctg ctgctgaatg cgcgctgcca gaagtttatt
    19801 gcgttgtggc gtaaaatgcg tcaccagctc agtcagaaat tctaccgctt cggcagtaag
    19861 aatttgcttc tcctgctcgc catacggcct tgtgaaagcc agttcatcgg ttgttgttgc
    19921 ctgttcagtc atcgtgcagc tcctcgtcat ggatccgaaa acttcccctc tgtgaacgaa
    19981 ggaatgttgt gcgcttttcg ttcagcacaa ctaagactac tcatttaaaa tgcaaaaaca
    20041 aaaacaattt ccattttaat taaaaatact tgataactta ttgataacaa tatgaataaa
    20101 atttattcac ttgctgaggt gcgtttcgga aataaaaaag gcacccgaag gtgcctgagg
    20161 taaggtgctg aatcgcttaa cgatcgacta tcacagaaga ttaatccagc gttggattca
    20221 tgtgccgtag atcgtatggc gtgatctgat agacgtaata gttgagccag ttggtaaaca
    20281 gcaaattacc gtgactacgc cagctcgctc gcggtttgtt ttgcggatcg ttgtgcggga
    20341 aatagttata cggtacatcc gggtctagtc cggcttccac atcgcggaaa tattcctgcg
    20401 ccagcgtttg cgcatcatat tcgggatggc ccgtcacaaa ggcaatgcgc ttatctttgc
    20461 tggcaaacag atatgcatcc ccttcttccg tctctgccag aatttccaga tcggtgtaat
    20521 cacgaatcaa cgctgccgga aagtcagcat agcgcgaatg cggtgccagg aatgaatcat
    20581 caaagccacg cgtcagaagc gcatgaggat ggagaatatg atgctcgtaa acgccagaga
    20641 gtttgtcggt gcgagtttgc ttaggaatgc cgtagaggat attgagcgcg gcctgtaccg
    20701 cccagcagac aaacagcgtc gaggtgacgt gatctttcga ccactccagc acctgtttga
    20761 tctgcggcca gtaagcgaca tcattaaact ccaccaggcc cagcggcgca ccggttacaa
    20821 tcagaccgtc aaagttctgt tcctgaatat cttcaaagtt acagtagaag ttgttcagat
    20881 gctctgcggg cgtgttgcgc gattcacggg aatcgatgcg caacagctga atatcgacct
    20941 gcaaaggtga gtttgaaagc aggcgcagaa actgattttc agtttcaatc ttcttcggca
    21001 tcaggttaag gatcagaacc ttaagtggac gaatttcctg accagacgca cgagaagttg
    21061 tcatcacaaa gacgttttct tcacgcaaga aattgacggc gggtagctcg tccggcacac
    21121 gaatcggcat aacctgatta cctcactaca tacgcttata cgtttagaca tccagatagc
    21181 tgaagataac cagaaaattt gccaatgtcg agcctgcatg ttgaaggtga gaaagtttca
    21241 aggaaaaggc tacgttagaa tataagaatg acgaaaagga gagaaaatgg ttattagtat
    21301 tcgccgctca cggcatgagg aaggggagga actcgttgcg atttggtgtc gatctgtcga
    21361 tgccacccac gattttttat cagcagagta tcgggccgag ctggaagagc tggtgcgttc
    21421 ctttctgccg gaagcaccgt tgtgggtcgc ggttaatgaa cgggagcagg cggttgggtt
    21481 ctataagaag gtgggtttta aggttacggg acgctctgag gtggacgatt tggggaaacc
    21541 gtatccgttg ctgaatctgg cgtatgtggg ggaataagcc gcccctgccg tgcctgccca
    21601 agctggagaa gacgcgccag cgtcgcaccc ggcatttttt tcac
//
LOCUS       DADMEQ010000059        21505 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.059, whole genome shotgun sequence.
ACCESSION   DADMEQ010000059 DADMEQ010000000
VERSION     DADMEQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.059"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="JNZ05_002295"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="JNZ05_002295"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAY8565513.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="JNZ05_002296"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="JNZ05_002296"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAY8565514.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEMEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="JNZ05_002297"
     CDS             complement(3121..3420)
                     /locus_tag="JNZ05_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565515.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /locus_tag="JNZ05_002298"
     CDS             3595..4590
                     /locus_tag="JNZ05_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8565516.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="JNZ05_002299"
     CDS             complement(4632..5069)
                     /locus_tag="JNZ05_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HAY8565517.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="JNZ05_002300"
     CDS             complement(5115..5456)
                     /locus_tag="JNZ05_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565518.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5726..7078)
                     /gene="xylB"
                     /locus_tag="JNZ05_002301"
     CDS             complement(5726..7078)
                     /gene="xylB"
                     /locus_tag="JNZ05_002301"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAY8565519.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMLALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGMWGQHWAQQGWRRLRRIQRNRSLSCCRSYR
                     "
     gene            complement(7150..8472)
                     /gene="xylA"
                     /locus_tag="JNZ05_002302"
                     /pseudo
     CDS             complement(7150..8472)
                     /gene="xylA"
                     /locus_tag="JNZ05_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8838..9830
                     /gene="xylF"
                     /locus_tag="JNZ05_002303"
     CDS             8838..9830
                     /gene="xylF"
                     /locus_tag="JNZ05_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8565520.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKEKEL"
     gene            9908..11449
                     /gene="xylG"
                     /locus_tag="JNZ05_002304"
     CDS             9908..11449
                     /gene="xylG"
                     /locus_tag="JNZ05_002304"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HAY8565521.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11427..12608
                     /gene="xylH"
                     /locus_tag="JNZ05_002305"
     CDS             11427..12608
                     /gene="xylH"
                     /locus_tag="JNZ05_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HAY8565522.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12686..13864
                     /locus_tag="JNZ05_002306"
                     /pseudo
     CDS             12686..13864
                     /locus_tag="JNZ05_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
     gene            complement(13972..14796)
                     /locus_tag="JNZ05_002307"
     CDS             complement(13972..14796)
                     /locus_tag="JNZ05_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HAY8565523.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15116..17146
                     /gene="malS"
                     /locus_tag="JNZ05_002308"
                     /pseudo
     CDS             15116..17146
                     /gene="malS"
                     /locus_tag="JNZ05_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            17324..18577
                     /gene="avtA"
                     /locus_tag="JNZ05_002309"
     CDS             17324..18577
                     /gene="avtA"
                     /locus_tag="JNZ05_002309"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HAY8565524.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRSLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18728..19201)
                     /locus_tag="JNZ05_002310"
     CDS             complement(18728..19201)
                     /locus_tag="JNZ05_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY8565525.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="JNZ05_002311"
     CDS             complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="JNZ05_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HAY8565526.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTHNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGALT"
     gene            20352..21350
                     /gene="yiaK"
                     /locus_tag="JNZ05_002312"
     CDS             20352..21350
                     /gene="yiaK"
                     /locus_tag="JNZ05_002312"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HAY8565527.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21362..>21505
                     /locus_tag="JNZ05_002313"
     CDS             21362..>21505
                     /locus_tag="JNZ05_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HAY8565528.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQ"
BASE COUNT         5405 a         5487 c         5279 g         5334 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccatttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccttgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaacctt tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 cccccgtacg gtaatcgagc tgctgaccgc tgatatccgc cagcatctga cgccagtact
     5881 cactacgcgc cccgccacca atcaacgtaa cactttgcgg tttaatgccg caggcatgca
     5941 cgacatccat gccatctgcc agcgcataac ccacgccttc cagcactgct cgcgccagtt
     6001 cattggggcc atgttgatga gtcaaaccaa agaaaacccc cttcgcctgg ggattattgt
     6061 gtggtgtacg ctcgccggaa agataaggca gaaaccaaac tggctcggca ctttcatcag
     6121 cctgttgagc tgcagcgatt aaagctggga cattgcacag gccggttaat ttcgcggccc
     6181 aatccagaca cgatgctgca ctcagcatta cagacattaa atgccaacgc tgcggtagcg
     6241 catggcaaaa gctgtgtaca gcgctttctg gcttgcttaa gaacccttcg ctgacagcaa
     6301 aatagacccc cgacgtcccc agcgataaca ttgcctgatt agcatcaacc attcccacac
     6361 caactgcacc agctgcattg tcgccaccgc ctgcgacaac tggcaccgtc gccatacccc
     6421 acgctttcgc aacttcaggt aacaaagcac cagtaatttc gctgccttcg tataatgcga
     6481 gcatctggtc acgagataag tcgcaagcct gcagcatgac gtcactccag tcacgctttg
     6541 cgacatccag ccacatggtg ccagctgcgt cagacatatc gctggcaaac tcccccgtca
     6601 tacgcagacg caagtaatct ttcggtaata atactttgtc gatttgacgg aatatctccg
     6661 gctcatgccg ctgaacccat agcaatttag gcgcagtaaa tccgggcatc atcaggttgc
     6721 cggtaatcac tcgtgattgc ggaactctcg cttccagcaa agtgcactct tgcgcacagc
     6781 gcccgtcgtt ccacaaaatg gcagggcgca atacccgttg ttgagcatcc agtaaggttg
     6841 ctccatgcat ctggccggca atacccaatg ctttaacgtc ctgcagagaa tgctgatcgc
     6901 ccagagcttt cattgcgcga tcagttgcct gccaccactg ttccgggtct tgttccgacc
     6961 aaagtggatg cgggcgcgaa acggtcagct tttccgtttg cgaagcaacc acctcaccct
     7021 gctcgttgag caaaataact tttacgcccg aggtgccaag atctatcccg atatacatat
     7081 cgatcgttcc ttaaaaaaat gcccggtatc gctaccgata accgggccaa cggactgcac
     7141 agttagccgt tatttgtcga acagataatg atttaccaga ttttccagtt gctcctggcg
     7201 accactctga tgcaccggag acaaattatg ttcctgagca tatttggcta aatctgccag
     7261 tgacatttgg cctttcagga tttgctggcc caattcgcta ttccagccgg aataacgctg
     7321 cgcgatgcgt ttatccagct cgccatcttc aatcatgcgc gctgcaattt tcagcgccag
     7381 tgccatcgta tccatcgcgc cgatatgacc gtaaaacaga tcatatttat cagtactttg
     7441 acgacgtact ttggcatcga agttcagacc accggtggtg aaaccgcctg ctttgagaat
     7501 ttcatacatc accagcgcat tctcttccac actgttcggg aactggtcgg tgtcccagcc
     7561 cagttgcgca tcgccacggt tggcgtcgac agaaccgaac aggccaagcg caatggcggt
     7621 ggctatttca tgatggaaag agtgacctgc cagcgtcgcg tggttagctt caatgttcag
     7681 tttaatctct ttttccagac caaactgttt caggaagcca tagaccgtcg cggcatcgta
     7741 atcatattga tgtttagtcg gttcttgcgg tttcggttcg ataagcaacg tgccctagaa
     7801 gccgatttta tgtttatgct caaccaccat ctgcataaag cggcccagtt gttcacgctc
     7861 ctgacgcaag tcggtattta acagcgtttc gtaaccttca cgaccacccc acaggacata
     7921 gttttcaccg cccaatttat gggttgcttc catcgctgta acaacttgcg ttgccgccca
     7981 gctgaagact tcaggatctg ggttcgtcgc cgcacccgcg ccatagcgag ggtttgtaaa
     8041 gcagttagcg gttccccaca gcagcttcac gccgctctct tcttgcttgg ctgccaggac
     8101 atcaaccatt tgcgcaaaat tattgatgta ctcttttaac gacgcgccct caggggaaac
     8161 atccacatcg tggaagcaat aaaatggcac atgtaacttg tggaaaaact caaatgcgac
     8221 atctgcttta cgcttcgcca acgccagtgc ctcaccaggc tgctgccacg gacgattaaa
     8281 cgcccccaca ccaaacatgt ccgccccgtt ccagcagaag gtgtgccagt agcaggcggc
     8341 aaaacgcaag tgctcttcca tacgcttacc caacaccagt tcgtcgggat tgtagtgacg
     8401 gaatgctaac gggtttgagg attttgagcc ttcataacga acgcgatcga gctggtcaaa
     8461 ataggcttgc atattgaact ccataatcag gtaatgccgc gggtgatgga tgatgtcgta
     8521 atattgggca ctccctttca attgctcaat tatgttattt cacactgcta ttgagataat
     8581 tcacaagtgt gcgctcgctc gcaaaataaa atgggatgat gaaactgggt aattcctcga
     8641 agataaaaat gcaataagta caattgcgca acaaaagtaa gatctcggtc ataaatcaag
     8701 aaataaacca aaaatcgtaa tcgaaagata aaaatctgta attgttttcc cctgtttagt
     8761 tgctaaaaat tggttacgtt tatcgcggtg attgttactt attaaagctg tcctctaact
     8821 acagaaggcc ctacaccatg aaaataaaga acattctact caccctttgc acctcacttc
     8881 tgcttaccaa cgttgcggca cacgccaaag aagtcaaaat aggtatggcg attgatgatc
     8941 tccgtcttga acgctggcaa aaagatcgag atatttttgt gaaaaaggca gaatctctcg
     9001 gtgcgaaagt atttgtacag tctgcaaatg gcaatgaaga aacacaaatg tcgcagattg
     9061 aaaacatgat taaccggggt gtcgatgttc ttgtcattat tccgtataac ggtcaggtat
     9121 taagtaacgt tgtaaaagaa gccaaacaag aaggtattaa agtattagct tacgaccgta
     9181 tgattaacga tgcggatatc gatttttata tttctttcga taacgaaaaa gtcggtgaac
     9241 tgcaggcaaa agccttggtc gatattgttc cgcaaggtaa ttacttcctg atgggcggct
     9301 cgccggtaga taacaacgcc aagctgttcc gcgccggaca aatgaaagtg ttaaaacctt
     9361 acgttgattc tggaaaaatt aaagtcgttg gtgaccaatg ggttgatggc tggttaccgg
     9421 aaaacgcatt gaaaattatg gaaaacgcgc taaccgccaa taataacaaa attgatgctg
     9481 tagttgcctc aaacgatgcc accgcaggtg gggcaattca ggcattaagc gcgcaaggtt
     9541 tatcagggaa agtagcaatt tccggccagg atgcggatct cgcaggtatt aaacgtattg
     9601 ctgccggtac gcaaactatg acggtgtata aacctattac attgctggca aatactgccg
     9661 cagaaattgc cgttgaattg ggcaatggtc aggagccaaa agcagatacc tcactgaata
     9721 atggcctgaa agatgtcccc tcccgcctcc tgacaccgat cgatgtgaat aaaaacaaca
     9781 tcaaagatac ggtaattaaa gacggattcc acaaagagaa agagctgtaa gcgttacgcc
     9841 ccggcgcgga gcgggggcgt gatttctctc catgccgcgt gaatgaattg gcttaggtgg
     9901 agtcgttatg ccttatctac ttgaaatgaa gaacattacc aaaaccttcg gcagtgtgaa
     9961 ggcgattgat aacgtcagct tgcggttgaa tgctggcgaa atcgtctcac tctgtgggga
    10021 aaatgggtct ggtaaatcaa cgctgatgaa agtgctgtgt ggtatttatc cccatggctc
    10081 ctacgaaggc gaaattattt ttgcgggaga agagattcag gcgagtcaca tccgcgatac
    10141 cgaacgcaaa ggtatcgcca tcattcacca ggaattggcc ctggtgaaag aattgaccgt
    10201 gctggaaaat atcttcctgg gtaacgaaat aacccacaat ggcattatgg attatgacct
    10261 gatgacgcta cgctatcaga agctgctcgc acaggtcagt ttatccattt cacctgatac
    10321 ccgcgttggc gatttagggc ttgggcaaca acaactggtt gaaattgcca aggcacttaa
    10381 taaacaggtg cgcttgttaa ttctcgatga accgacagcc tcattaactg agcaggaaac
    10441 gtcggtttta ctggatatta ttcgcgatct acaacagcac ggtatcgcct gtatttatat
    10501 ttcgcacaaa ctcaacgaag tcaaagcgat ttccgatacg atttgcgtta ttcgcgacgg
    10561 acagcacatt ggtacgcgtg atgctgccgg aatgagtgaa gacgatatta tcaccatgat
    10621 ggtcgggcga gagttaaccg cgctttaccc taatgaacca cataccaccg gagatgaaat
    10681 attacgtatt gaacatctga cggcatggca tccggtcaat cgtcatatta aacgagttaa
    10741 tgatgtctcg ttttccctga aacgtggcga aatactgggt attgccggac tcgttggtgc
    10801 cggacgtacc gagaccattc agtgcctgtt tggcgtgtgg cccggacaat gggaaggaaa
    10861 aatttatatt gatggcaaac aggtagatat tcgtaactgt cagcaagcca tcgcccaggg
    10921 gattgcgatg gtacccgaag acagaaagcg cgacggcatc gttccggtaa tggcggttgg
    10981 taaaaatatt accctcgccg cactcaataa atttaccggt ggcattagcc agcttgatga
    11041 cgcggcagag caaaaatgta ttctggaatc aattcagcaa ctcaaagtta aaacgtcgtc
    11101 ccccgacctt gctattggac gtttgagcgg cggcaatcag caaaaagcga tcctcgcccg
    11161 ctgtctgtta cttaacccgc gcatcctcat tcttgatgaa cctaccaggg gtattgatat
    11221 tggcgcgaaa tatgagatct acaaattaat taaccaactc gtccagcagg gtattgccgt
    11281 tattgtcatc tcttccgaat tacctgaagt gctcggcctt agcgatcgtg tactggtgat
    11341 gcatgaaggg aaactaaaag ccaacctgat aaatcataac ctgactcagg agcaggtgat
    11401 ggaagccgca ttgaggagcg aacatcatgt cgaaaagcaa tccgtctgaa gtgaaattgg
    11461 ccgtaccgac atccggtggc ttttctgggc tgaaatcact gaatttgcag gtcttcgtga
    11521 tgattgcagc tatcatcgca atcatgctgt tctttacctg gaccaccgat ggtgcctact
    11581 taagcgcccg taacgtctcc aacctgttac gccagaccgc gattaccggc atcctcgcgg
    11641 taggaatggt gttcgtcata atttctgctg aaatcgacct ttccgtcggc tcaatgatgg
    11701 ggctattagg tggcgtcgcg gcgatttgtg acgtctggtt aggctggcct ttgccactta
    11761 ccatcattgt gacgttggtt ctgggactgc ttctcggtgc ctggaacgga tggtgggtcg
    11821 cgtaccgcaa agtcccttca tttattgtca ccctcgcggg catgttggca tttcgcggca
    11881 tactcattgg catcaccaac ggcacgactg tatcccccac cagcgccgcg atgtcacaaa
    11941 ttgggcaaag ctatctcccc gccagtaccg gcttcatcat tggcgcgctt ggcttaatgg
    12001 cttttgttgg ttggcaatgg cgcggaagaa tgcgccgtca ggctttgggt ttacagtctc
    12061 cggcctctac cgcagtagtc ggtcgccaag ctttaaccgc tatcatcgta ttaggcgcaa
    12121 tctggctgtt gaatgattac cgtggcgttc ccactcctgt tctgctgctg acgttgctgt
    12181 tactcggcgg aatgtttatg gcaacgcgga cggcatttgg acgacgcatt tatgccatcg
    12241 gcggcaatct ggaagcagca cgtctctccg ggattaacgt tgaacgcacc aaacttgccg
    12301 tgttcgcgat taacggatta atggtagcca tcgccggatt aatccttagt tctcgacttg
    12361 gcgctggttc accttctgcg ggaaatatcg ccgaactgga cgcaattgca gcatgcgtga
    12421 ttggcggcac cagcctggct ggcggtgtgg gaagcgttgc cggagcagta atgggggcat
    12481 ttatcatggc ttcactggat aacggcatga gtatgatgga tgtaccgacc ttctggcagt
    12541 atatcgttaa aggtgcgatt ctgttgctgg cagtatggat ggactccgca accaaacgcc
    12601 gttcttgatt ttgataaaaa ttttctcaaa gccggttacg tattaccggt tttgagtttt
    12661 tgcatgattc agcaggaaaa gaaccatgtt tactaaacgt caccgcatca cattactgtt
    12721 caatgccaat aaagcctatg accggcaggt agtagaaggc gtaggggaat atttacaggc
    12781 atcacaatca gaatgggata ttttcattga agaagatttc cgcgcccgca ttgataaaat
    12841 caaggactgg ttaggagatg tcgtcattgc cgacttcgac gacaaacaga tcgagcaagc
    12901 gctggctgat gtcgacgtcc ccattgttgg ggttggcggc tcgtatcacc ttgcagaaag
    12961 ttacccaccc gttcattaca ttgccaccga taactatgcg ctggttgaaa gcgcattttt
    13021 gcatttaaaa gagaaaggcg ttaaccgctt tgctttttat ggtcttccgg aatcaagcgg
    13081 caaacgttgg gccactgaac gcgaatatgc atttcgtcag cttgtcgccg aagaaaagta
    13141 tcgcggagtg gtttatcagg ggttagaaac cgcgccagag aactggcaac acgcgcaaaa
    13201 tcggctggca gactgactac aaacgctgcc accgcaaacc gggattattg ccgttactga
    13261 cgcccgagcg cggcatattc tgcaagtatg tgaacatcta catattcccg taccggaaaa
    13321 attatgcgtg attggcatcg ataacgaaga actgacccgc tatctgtcgc gtgtcgccct
    13381 ttcttcggtc gctcagggcg cgcggcaaat gggctatcag gcggcaaaac tgttgcatcg
    13441 attattagat aaagaagaaa tgccgctaca gcgaattttg gtcccaccag ttcgcgtcat
    13501 tgaacggcgc tcaacagatt atcgctcgct gaccgatccc gccgttattc aggccatgca
    13561 ttacattcgt aatcacgcct gtaaagggat taaagtggat caggtactgg atgcggtcgg
    13621 gatctcgcgc tccaatcttg agaagcgttt taaagaagag gtgggtgaaa ccatccatgc
    13681 aatgattcat gccgagaagc tggagaaagc gcgcagtctg ctgatttcaa ccaccttgtc
    13741 gatcaatgag atatcgcaaa tgtgcggtta tccatcgctg caatatttct actctgtttt
    13801 taaaaaagca tatgacacaa cgccaaaaga gtatcgcgat gtaaatagcg aggtcatgtt
    13861 gtagcgatga tgagaattgt cggcgtcaca tcagggaact cagcacagcc acctgatgcg
    13921 agcaaaagcc ttatccgacc gacaaatcag ataaggctcg aggaaatgaa attacatatg
    13981 cgccgcgatt aaccgttggt tatcctggta cattgcgaac aggtagttgt tataactctt
    14041 ccctttggtc gagtagccct tcagcttgtg aatcatcgct gtggcagtca cttcctgatc
    14101 cgctttacgc agctgtgcac gcgatttacg gaacgaagag taagccgggt gcgtattcag
    14161 gttagtgaca taggcgctca ccgattcttt gacagaacta aactgtgagt accctttcac
    14221 tttacccggc gcattggtac aacgtccttt catgcatttc atgccgaaca gattgttatt
    14281 gttgcgcgcc agcttcgacg ttccccaacc gctttctgct gcagccatcg tcgccaccat
    14341 actggtaggg ataatgtcta cgcgttcaag caaggtattc cacgggattt ttcgcgtatt
    14401 accagaccac ttcaccttgt agcgtttggc gatgtctttc agacgcgcac gctcagcagg
    14461 tgaccattgg ccctgatact gttttgaaat cagccagtta cgttctgcag taatggctgc
    14521 attttggctg gtaatgtaag gcattacggt ccggagaaac gcctttttcc ttggtgttcc
    14581 ggaagggtat tttcgcaaat caggaagtga actactcttt gcactattgc gagaatactc
    14641 ttgtttactg cttacctgtt tattactagc tttagttaag tgggactttt gactcgctgt
    14701 tgttgtgtgc gtcttcgcta acacctcact cgaaaacacc agagtgagta acataagaat
    14761 catcgcccca tatcgtcgta tgggagtcaa aatcatcagg tctcctggtc ggatttaatc
    14821 attccaacac cttatatttt tcacaaattt gagagttgaa tctcaaatca tatcaaaaat
    14881 agctgtcaag agcaccccaa ggaatagtcc aaatctgaaa ctatgtcacg agttaacgat
    14941 tcagattggc gctaaatcgc agaaaatgtg ggggttatcg caaaattcag ccgttttttg
    15001 cgcgcgatcg ctcacccttg cttctcatcc tgtggactta ccgctcaggg atgagttttg
    15061 tctggcttat cgctggcaaa ctgtctgaaa tcgcagcaat aaggactcat ccaccatgaa
    15121 actcgccgcc tgttttctga cactccttcc tggcttcgcc gttgccgcca gctggacttc
    15181 tccggggttc cctgccttta gcgaacaggg aacgggaaca tttgtcagcc acgcgcagtt
    15241 gcccaaaggt acgcgtccac tcacgctaaa ttttgaccaa cagtgctggc agcctgcaga
    15301 tgcgataaaa ctcaatcaga tgctttccct gcaaccttgt agcaacacgc cgcctcaatg
    15361 gcgattgttc agggacggca aatatacgct gcaaatagac acccgctccg gtacgccaac
    15421 attgatgatt tccatccaga acgccgccga accggtagca aacctggtcc gtgaatgccc
    15481 gaaatgggat ggattaccgc tcacgctgga tgtcagcgcc actttcccgg aaggagccgc
    15541 cgtacgggat tattacagcc aacaaattgc gatagtgaag aacggtcaaa taacgttaca
    15601 acccgctgct accagcaacg gtttactcct gctggaacgg gcagaaactg acacatccgc
    15661 ccctttcgac tggcataacg ccacggttta ctttgtgctg acagatcgtt tcgaaaacgg
    15721 cgatcccagt aatgaccaga gttacggacg tcataaagac ggtatggcgg aaattggcac
    15781 ttttcacggc ggcgatttac gcggcctgac caacaaactg gattacctcc agcagttggg
    15841 cgttaatgct ttatggataa gcgccccatt tgagcaaatt cacggctggg tcggcggcgg
    15901 tacaaaaggc gatttcccgc attatgccta ccacggttat tacacacagg actggacgaa
    15961 tcttgatgcc aatatgggca acgaagccga tctacggacg ctggttgata gcgcacatca
    16021 gcgcggtatt cgtattctct ttgatgtcgt gatgaaccac accagctatg ccacgctggc
    16081 ggatatgcag gagtatcagt ttggcgcgtt atatctttct ggtgacgaag tgaaaaaaac
    16141 gctgggtgaa cgctggagcg actggaaacc tgccgccggg caaacctggc atagctttaa
    16201 cgattacatt aatttcagcg acaaaacagg ctgggataaa tggtggggaa aaaactggat
    16261 cagaacggat atcggcgatt acgacaatcc tggattcgac gatctcacta tgtcgctggc
    16321 ctttttgccg gatatcaaaa ccgaatcaac taccgcttct ggtctgccgg tgttctataa
    16381 aaacaaaacg gatactcacg ctaaagtcat cgaaggcttt acacctcgcg attacttaac
    16441 ccactggtta agtcagtggg tccgcgacta tgggattgat ggttttcggg tcgataccgc
    16501 caaacatgtt gagttgcccg cctggcagca actgaaaacc gaagccagct ccgcgcttcg
    16561 cgaatggaaa aaaactaacc ccgacaaagc attagatgac aaacctttct ggatgaccgg
    16621 tgaagcctag ggccacggcg tgatgcaaag tgactactat cgccacggct tcgatgcgat
    16681 gatcaatttc gattatcagg agcaggcggc gaaagcagtc gattgtctgg cgcagatgga
    16741 tacgacctgg cagcaaatgg cggagaaatt gcagggtttc aacgtgttga gctacctctc
    16801 gtcgcatgat acccgtctgt tccgtgaagg gggcgacaaa gcagcagagt tattactatt
    16861 agcgccaggc gcggtacaaa tcttttatgg cgatgaatcc tcgcgtccgt tcggtcctac
    16921 aggttctgat ccgctgcaag gtacacgttc ggatatgaac tggcaggatg ttagcggtaa
    16981 atctgccgcc aacgtcgcgc actggcagaa aatcagccag ttccgcgccc gccatcccgc
    17041 aattggcgcg ggcaaacaaa cgacactttc gctgaagcag ggctacggct ttgttcgtga
    17101 gcatggcgac gataaagtgc tggtcatctg ggctgggcaa cagtgacttt tccggcttcc
    17161 cgttcgtcag tacctcggga agccgccaac caggataaaa tgtcagccct aatcagcgtt
    17221 gcgggataaa gcaccgctca ctcttcaaca gaccgatttg caccccagca aatgtagcgt
    17281 tattgttacc ttccttgcta cagagttcga cagatatccc gctatgacat tctccctttt
    17341 tggtgacaaa tttacccgcc actccggcat tacgctgctg atggaagatc tgaacgacgg
    17401 tttacgcacg cctggcgcga ttatgctcgg cggcggtaat ccggcgcaga tcccggaaat
    17461 gcaggactac ttccagacgc tactgaccga catgctggaa agtggcaaag cgactgatgc
    17521 actgtgtaac tacgacggtc cacaggggaa aacggagcta ctcacactgc ttgccggaat
    17581 gctgcgcgag aagttgggtt gggatatcga accacagaat attgcactaa caaacggcag
    17641 ccagagcgcg tttttctact tatttaacct gtttgccgga cgccgtgccg atggtcgggt
    17701 caaaaaagtg ctgttcccgc tggcaccgga atacattggc tacgctgacg ccggactgga
    17761 agaagatctg tttgtctctg cgcgtccgaa tattgaactg ctgccggaag gccagtttaa
    17821 ataccacgtc gattttgagc atctgcatat tggcgaagaa accgggatga tttgcgtctc
    17881 ccggccgacg aatccaacag gcaatgtgat tactgacgaa gagttgctga agcttgacgc
    17941 gctggcgaat caacacggca ttccgctggt gattgataac gcttatggcg tcccgttccc
    18001 gggtatcatc ttcagtgaag cgcgcccgct atggaatccg aatatcgtac tgtgcatgag
    18061 tctttccaag ctggggctgc ctggctcccg ctgcggcatt atcatcgcca atgaaaaaat
    18121 catcactgcc atcaccaata tgaacggcat tatcagcctg gcacctggcg gtattggtcc
    18181 ggcgatgatg tgtgaaatga ttaagcgtaa cgatctgctg cgcctgtctg aaacagtcat
    18241 caaaccgttt tactaccagc gtgttcagga aactatcgcc atcattcgcc gctatttacc
    18301 ggaaaatcgc agcctgattc ataaaccgga aggagccatt ttcctctggc tatggtttaa
    18361 ggatttgccc attacgaccg agcagctcta tcagcgcctg aaagcacgcg gcgtgctgat
    18421 ggtgccgggg cacaacttct tcccgggact ggataaaccg tggccgcata cgcatcaatg
    18481 tatgcgcatg aactacgtac cagagccgga gaaaattgag gcgggggtga agattctggc
    18541 ggaagagata gaaagagctt gggcagaaag tcactaaaaa ttgcctgatg cgctacgctt
    18601 atcaggccta catgatttct gcaatatatt gaatttggaa gaatttgtag gccggataag
    18661 gcgtttacgc cgcatctggc ataaacaaag cgcactattc cggcgttgag aaacgccgga
    18721 aaacgtttca aacaggctgc tgctgtagcc gatgccgctg cactttctcg acatccatgc
    18781 actgcaacgc ctttgtcggg caggcttcaa cacaggccgg ccccgtctcc cgatgccagc
    18841 acagatcgca cttaatcgcc ctcgcctttt tgcgcgaaga aacgacctcc atcgcaccaa
    18901 acgggcaagc cagcatacag cttttacagc caatgcaacg tgtttgttca acgaaaatat
    18961 gcccatgttc gcggcttatc gcgtcaacag ggcagacatt cgcgcacggt gcatcttcac
    19021 actgatgaca ggctactggc gtggtccagc tgtggtcttt aatgacacga atacgggaaa
    19081 taaactcgtc tggtgacaac gcagcgcaat cctgattctc ctgatgcgac actgcgcaag
    19141 ccacttcaca ggtacggcaa ccgatacatt tcgtcgcatc cgcaataata aaccggttca
    19201 tgatcattcc tttgcactaa aacagcagag tgccagagag cgcgtcccta ctgctttgat
    19261 ccaacgaagg aaatgttaaa gttttggcac ttgtccaaag cgttatgtga gcgcgcccag
    19321 gtcatccctg acagtaaatc ccagttcatt agaaatagcc tgcgcggttt cacgcagtgg
    19381 tttcaggaga tttttctctc ccacctgttt cagacgtgat gtcgaaagcg aaatcgacac
    19441 ggcgtacggc acccgcccat gaatatcaaa caccggaaca gcaatacagg agacgccgag
    19501 ttcgttttct tctctgtcca tcgccgctcc gctttcacga atgtgcgcca gttcgtcgaa
    19561 catcgcgggc agctcggtaa tggtattgtg ggttaacggc tggatctcat gctgatggtt
    19621 ttcccagtat gacttcacgt agtccgggtg accaaacgcc atgtagatct tgcccattgc
    19681 ggaacagtag agcggcatat gctggccaat ataggcacgg gttcgcagca tcccggttgt
    19741 gggttccagc ttataaatca aaatagcgtg atcgtcttcg cggctggaga agttaatggt
    19801 ttcaccagtg gcgatgttca gtgcctcaag atgcggagcg gcgatatgaa tgatattcag
    19861 cgaagacagc gccttctgcc cgacggcaat aaatttggta gtcaggcggt aactccctgc
    19921 ggcgggcgcg gtggtcacat agccgcagga ctgtaatccc tgcaataagc gatggacggt
    19981 actcttattt aaaccagcca gctccgaaag atgcgccaac ggacaaccgt ttggatagtt
    20041 gctcaaaatc tcaatcagca tcaacccgcg aaacagactc tggcttccgg ctggacgctc
    20101 tttttcctgc gccatctcgt tctctttttt tcccatcact tctttcccca ttttgtcgcg
    20161 tcctgatggt agcgcaaagt gtgccgtagt tcacgatctc gacagataat ttataaccac
    20221 ttgattttta tgtcttttga aattcatcaa tcagattgct ttgttaaaaa gtgatcgata
    20281 tatttgaaat ctagtttcgc atattgaaat tttaagccaa aaaagcgatc aaaaaaacaa
    20341 ggaagcctag gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa
    20401 tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca
    20461 ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg
    20521 aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg
    20581 aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg
    20641 ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact
    20701 ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct
    20761 ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc atcggcacta
    20821 acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga
    20881 tgttctctta cggcatgtta gaagttaacc gcctggcagg ccgtcagctc ccggtcgatg
    20941 gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc
    21001 gcattttgcc gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg
    21061 ctactctcct ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat
    21121 acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc
    21181 gcgatgccaa gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa
    21241 accaggccat ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta
    21301 acggcatcac tgttgatgac agcgtgtggg ccaaaatcca ggcgttatga ggagataagt
    21361 catgattttt ggacatatcg cgcagcccaa tccgtgccgt ttgcccgccg ccattgaaaa
    21421 ggcgctcgat tttctgcgcg ctaccaactt caacgtcctg gagccgggcg ttgtcgaaat
    21481 cgacggcaag aatatttatg cgcaa
//
LOCUS       DADMEQ010000060        21359 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.060, whole genome shotgun sequence.
ACCESSION   DADMEQ010000060 DADMEQ010000000
VERSION     DADMEQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.060"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..76
                     /locus_tag="JNZ05_002314"
     CDS             <1..76
                     /locus_tag="JNZ05_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY8565529.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            92..1018
                     /locus_tag="JNZ05_002315"
                     /pseudo
     CDS             92..1018
                     /locus_tag="JNZ05_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin"
     gene            1257..1316
                     /locus_tag="JNZ05_002316"
     CDS             1257..1316
                     /locus_tag="JNZ05_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY8565530.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1633..1692
                     /gene="ibsE"
                     /locus_tag="JNZ05_002317"
     CDS             1633..1692
                     /gene="ibsE"
                     /locus_tag="JNZ05_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HAY8565531.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1812..3245)
                     /gene="hldE"
                     /locus_tag="JNZ05_002318"
     CDS             complement(1812..3245)
                     /gene="hldE"
                     /locus_tag="JNZ05_002318"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HAY8565532.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3293..6133)
                     /gene="glnE"
                     /locus_tag="JNZ05_002319"
     CDS             complement(3293..6133)
                     /gene="glnE"
                     /locus_tag="JNZ05_002319"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HAY8565533.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAAAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6156..7457)
                     /locus_tag="JNZ05_002320"
     CDS             complement(6156..7457)
                     /locus_tag="JNZ05_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HAY8565534.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQPLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7699..8319
                     /locus_tag="JNZ05_002321"
     CDS             7699..8319
                     /locus_tag="JNZ05_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAY8565535.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPNRKRKDRWMN"
     gene            8383..9621
                     /gene="cca"
                     /locus_tag="JNZ05_002322"
     CDS             8383..9621
                     /gene="cca"
                     /locus_tag="JNZ05_002322"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HAY8565536.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9784..10605)
                     /gene="bacA"
                     /locus_tag="JNZ05_002323"
     CDS             complement(9784..10605)
                     /gene="bacA"
                     /locus_tag="JNZ05_002323"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY8565537.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10696..11064)
                     /gene="folB"
                     /locus_tag="JNZ05_002324"
     CDS             complement(10696..11064)
                     /gene="folB"
                     /locus_tag="JNZ05_002324"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HAY8565538.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11169..11786
                     /gene="plsY"
                     /locus_tag="JNZ05_002325"
     CDS             11169..11786
                     /gene="plsY"
                     /locus_tag="JNZ05_002325"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAY8565539.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11799..12730)
                     /gene="ttdR"
                     /locus_tag="JNZ05_002326"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(11799..12730)
                     /gene="ttdR"
                     /locus_tag="JNZ05_002326"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            12937..13780
                     /gene="ttdA"
                     /locus_tag="JNZ05_002327"
                     /pseudo
     CDS             12937..13780
                     /gene="ttdA"
                     /locus_tag="JNZ05_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            13777..14382
                     /gene="ttdB"
                     /locus_tag="JNZ05_002328"
     CDS             13777..14382
                     /gene="ttdB"
                     /locus_tag="JNZ05_002328"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HAY8565540.1"
                     /translation="MKKILTTSIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14430..15893
                     /locus_tag="JNZ05_002329"
     CDS             14430..15893
                     /locus_tag="JNZ05_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HAY8565541.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLLLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYPPTMVMVTLIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(15936..16949)
                     /gene="tsaD"
                     /locus_tag="JNZ05_002330"
     CDS             complement(15936..16949)
                     /gene="tsaD"
                     /locus_tag="JNZ05_002330"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAY8565542.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNSTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17187..17402
                     /gene="rpsU"
                     /locus_tag="JNZ05_002331"
     CDS             17187..17402
                     /gene="rpsU"
                     /locus_tag="JNZ05_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAY8565543.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17513..19258
                     /gene="dnaG"
                     /locus_tag="JNZ05_002332"
     CDS             17513..19258
                     /gene="dnaG"
                     /locus_tag="JNZ05_002332"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY8565544.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVIFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTSGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19453..21294
                     /gene="rpoD"
                     /locus_tag="JNZ05_002333"
     CDS             19453..21294
                     /gene="rpoD"
                     /locus_tag="JNZ05_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAY8565545.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5155 a         5897 c         5484 g         4823 t
ORIGIN      
        1 actgtcgttc tcaaaatcgg tggagttgca tgacaaggtc atcgggcatt atctgaacat
       61 aaaacactat cagtaagttg gagtcattac cgaacaacaa gaagcgttta tgacgcttga
      121 gcaggagcag caggttaaaa cccgtactgc cgaacagaat gcacgtattg cggcttttga
      181 agctgaacgt cgtcgtgaag cagagcagac gcgaattctg gctgaacgac aaattcagga
      241 aacggagatc gagcgcgagc aggccgtccg ctcaagaaag gttgaggctg aacgtgaagt
      301 tcgtattaaa gagatcgaac agcagcaggt caccgaaatc gccaaccaga cgaaatcgat
      361 cgctattgcc gccaaatcgg aacaacagtc ccaggcagaa gcgcgtgcta accttgcact
      421 cgcagaagca gtaagtgcgc aacaaaacgt agaaaccact agccagaccg cagaagccga
      481 tcgtgctaaa caagttgccc taatcgctgc cgcgcaggat gcagaaacca aagcggttga
      541 actgaccgtg cgggcgaaag cagaaaaaga agccgcagag atgcaggcgg cggctatcgt
      601 tgagttagcc gaagctacac gtaaaaaggg tctggcggaa gcagaagcac aacgtgcgct
      661 gaacgatgct atcaacgtac tttctgatga acaaaccagc cttaaattca aactggcctt
      721 gttgcaggcg ctacctgcgg taatagaaaa atccgttgag ccgatgaaat ctattgacgg
      781 tatcaagatt attcaggtcg atggtctgaa tcgtggcagc gctgcgggtg atgcaaacac
      841 gggtaatgtg gggggcggaa acctagcgga acaagcatta tcagccgctc tctcttaccg
      901 cacacaggct ccgctgattg actccttgct caatgaaatt ggcgtttcag gcggttcact
      961 ggcgacattg acttcaccct taacctcaac aactcccgtc gccgaaaacg tagaataaat
     1021 tttcagctat gaacgtccct ggcacaaaaa gccggggatt ttttatatct gcgttccgct
     1081 aaaaactgca aatgatcagg tcgttgcaac gttttgcgtg accgctcggg gaaggcaaaa
     1141 ctgcctctgg gaaagcgttg cgcgaggtcc ggcgctcatc aacaatcggg gagtcagcaa
     1201 agggctgaaa cgggaaagcc cctcccgagg aaggggcctt acagaaggaa agggttatga
     1261 tgaagctcgt catcatactg attgtgttgt tactcgtaag tttcgcagct tattaacagc
     1321 caatcagagg ggggagaaat cctccctcac ccttgttcct ttactttagg ttgaaaaaaa
     1381 atcagcgtca agaaggtttc attaacacat atccctggcc caaaaagccg gggatttttt
     1441 atatctgcgt tccgctaaaa ggtgcaaatg ctcaggacgt tgcagcgttt tgcgtgaccg
     1501 ctcggggaag gcaaaactgc ttcagggaaa gcgttgcgcg gggtccggcg ctcatcaaca
     1561 atcggggagt cagcaagggg ctgaaacggg aaagcccctc ccgaggaagg ggccttatag
     1621 aaggaaaggg ttatgatgaa gttcgtcatc atactggttg tgttgttact gttaagtttc
     1681 ccgacttact aacaactgat cagagggggg agaaatcctc ccttaccctt gttcctttac
     1741 tttaggttga aaaaacaaca gcgtcaatag gcctgcctgc tacgaagcga gatctgtgaa
     1801 ccgctttccg gttagccttt tttatcctgt tggatcttct tgatgatgtt agtggtcgag
     1861 caaccgtctt cgaagttgag caccagcact tcgccgccgt tggcccagac ttctttactc
     1921 ccggcgatct cttctggttt atagtcgccg cccttcacca gtaaatctgg caggatcccg
     1981 gcgatcaagc gctgcggcgt gtcttcttca aacgacacca cccagtcgac cgcttccagt
     2041 gcgcccagca caatcatacg ctgttcgagc gggttaacgg ggcgggaatc ccctttcagc
     2101 cgtttggtgg aggcatcgct gttcacagcg acaatcaagc ggtcacccag tttgcgggca
     2161 tttgccagat agaagacgtg tccggcgtgc aggatgtcaa agacgccatt ggtcatcacc
     2221 actttttcac cgcgtttacg cgctgccgct acggccagct tcagttcctc ttcggtcatc
     2281 acgccaaagc cggtatctgc gcgcccacgc actgcatttt ccagctcgat cggcgaaacc
     2341 gtggaggttc ccagtttgcc gaccaccacg ccagccgccg cattggcaaa gaagcaggct
     2401 tcttccagcg aattacccgc tgccagcgtt gccgccagga cgccaatcac cgtgtcgccc
     2461 gcaccggtaa cgtcatacac ttcctgcgcc tgggttggca tatgcagcgg cgctttaccc
     2521 ggttgcagca gcgacatacc ctgttcggaa cgggtcacta acagagccga gagttcgtaa
     2581 tcggcaatca gtttcatgcc gcgctcaaca atctcttctt cggtcttaca tttaccgaca
     2641 acagcttcaa attccgagag attcggtgtc agcagcgtag cgccgcggta gcgctcaaaa
     2701 tcggtacctt ttggatcaat cagcaccgga acgcccgctt tacgcgccag ttggatcatc
     2761 tgctgtacgc ttgccagcgc acctttggcg tagtcagaaa gcaccagcgc gccaatcgaa
     2821 ctcagcgcct gattaatccg ctcgtgcagc ggctgcggat caacaccttc gaaaccttct
     2881 tcaaaatcca gacggatcag ctgttggttg cgggaaagta cccgtaattt ggtgatggtc
     2941 ggatgcgtcg gtacagaaac gaagtcgcat ttgacgttga cgtcggccag agatttactc
     3001 agcgcgcgcg ctgcatcgtc aatgcccgtc aacccgacca ggcgtgcatt agcaccgaga
     3061 gaagcgatat tcatcgccac gttagccgcg ccgcccggac gttcttcgat ggtattcact
     3121 ttaaccacgg gcaccggcgc ttccggcgag atacgactgg tggggccgta ccagtaacga
     3181 tccagcatca catcaccaac caccatcact cctgcacgtt caaactctgg cagcgttact
     3241 ttcattcctg tctcctgaga gattcaaaat ttgcgcgcga taataccata cttcattctt
     3301 ccaccagcca cttctgccag cttgcccgca ccagatcacg ctctgcggtg aagcaatcct
     3361 ccgacacatg gcccggcaat tcctgtaatg ccagatgatg aagttcatcg cgcagcgtcg
     3421 tgtaagcacg ggtcagcgcc atcgcttcct gctcttccat aatgtcgttt tgcgccagca
     3481 gctccagaat acgcacgttg tctgaccagc gcgttaactt cggtttttca tgagcgtagc
     3541 gcaacaccag atattgggta ataaattcga tatcggtaat tccgccttca tcggctttga
     3601 tatcaaagcg attacgatgt ttattgccga gatgagcgcg cattttctcg cgcatttccc
     3661 gcacttccgt ttgcagagtt ttaccttctc gcggcagtgt cataatctca cgacgcaccg
     3721 cgtcaaagtg cgcagtgagc tgcggatcgc cgtacactac acgcgcacgc accagcgcct
     3781 gatgttccca cgtccaggcc tcgtttttct gataatcggc aaatgctgct gcggatgtca
     3841 ccagcattcc cgccgccccg gacggacgca gtcgagcatc cacttcatac aaaatgccgg
     3901 aagaggtacg cgtactgaac agatgcataa tgcgttgcgc cagacgcaga taaaactgcc
     3961 gcccgtcgat ttcccgctcg ccgtcagtca tcgcatccat tgggcaatca tggaggaaga
     4021 taaggtcaag atcggaactg tagcctaact cccagccgcc cagcttgccg tagccgacca
     4081 ccgcaaaacc gcgcccttca cgttcgttga ggtgatttgg cttaccgtag cgggccacca
     4141 tttgacccca cgcctgctga acgacggcat ctatcatagc ttctgccagc caggttaagt
     4201 gatcgctcac tttcattacc ggtagcgtac cggcgatatc cgctgccgca atgcgtaaca
     4261 gctgcgcctg tttgaactga cgcagcgcct caagctgttg ttcttcgtca tcttccggca
     4321 cgcgcagcaa atactggcgc agttcatcac ggtaggcatc ggtcgccgtc ggctggtaaa
     4381 gggtatttgg atcgagcaat tcatccagca ataatggata acgcgctagc tggctggcaa
     4441 tcatcggcga tgcggcacac agagaaatca aatgtttaag cgccgcaggg aattcactga
     4501 gcaattctaa ataggttgtg cgggtaacaa tccccaccag caaggcggta atgcgcgaca
     4561 gcgtgacagc agcgtcttca cgcgcacaga catcacttag cagatgtggc atcagatggt
     4621 cgagcacctg acgtcctcgc ggcccgatag tacgcttatc cagctctttg cggaaatcag
     4681 caatcagcgt taggacctgt ttgcgatcat cctcgctaag atgcgccagc actggcgtag
     4741 tgtcatcttc ctgcaacgca tcctgccaca gctcacgcca ctgttccgac agcgactctt
     4801 cctgagtttc actttcgtcg tcgccaatca attcattaaa cacccggcgc acattggtca
     4861 tatgtgcggt cagcgccccg gtcagttgcg gccagtcagc aaagtccatc gcccacgcca
     4921 gccgcgcacg gttaagctca tcagaaggaa gcgtttgggt ttgttcgtcg ttaatgcttt
     4981 gcagcaggtt ttccagacgc cgcaggaaca gatatgccac tcgcaattgt tcagcatcgt
     5041 tttcagaaag cagatgcagc gcggcaatgg cgctgagcgt tggcagtaaa gagcgcgact
     5101 gcagcaacgg ttcgcgccca ccgcgaatga gctggaacac ctgaacgata aattcaattt
     5161 cgcgaatgcc gcctgcgccg agtttgatat tgtcggtcaa accacgtcga cgcacttcac
     5221 gggcaatcat ccctttcatg ttgcgcagcg actgaatcac gctgaaatcg atgtaacgac
     5281 ggaaaacaaa cgggcgcagc atcgcacgca actcgttagc atagacgcct tcgctatcgc
     5341 ccataatccg cgctttgacc atcgcgtaac gctcccagtc gcgtccctgc tcctggtaat
     5401 aatcctccaa cgcggcaaag ctcagcacca gcgggccact ttcgccaaac ggacgcagtc
     5461 gcatatccac gcgatagacg aagccatcct gcgttggttg atccagcact ttaatcagcc
     5521 gctgccccat gcgggtaaaa aactgcgcgt tatccagttc ccggcgtccg ccctgcgtac
     5581 aaccatgttc cggccaggca aaaatcagat cgatatcaga ggagaaattt agttccccgc
     5641 cgcccagctt acccatgcct aagatcagca gcggttgcgc ttcgccctgc gcattgcacg
     5701 gcgttcccca ctcgcggcag caggcgtcat acagccagtc acgcgccgca acaatcagcg
     5761 tctccgccag ataactgagc tgctgcaata tgctctcttc agtaaccagt gccagcgttt
     5821 gcgcccatgc gatgcgcacc ataatgcgcc gccggaatag ccgcagctcg cgcattaacc
     5881 cggcttcgtc gctcacatta ctgagcgcct cctgcaacca cacgctgtaa tgttgccatt
     5941 cgtcagcctg tggcggttgg ctttccagtt ccgtcagcca ctctggatgc gcaatcacgc
     6001 tgtcctgcac aaaatcactg aaagtaagta ctgacttcgc ctgtgcacta agtgattcct
     6061 cggctaaaag ctctggcagc cgctcaacaa cggtctgcca gtactgctgt aacggtgaag
     6121 agagcggctt cataagcgat tttatccttg cctgattaac gttttccgct gtgcaaccag
     6181 aacggttcct gattgtttgc ctcattacgg aaatgttcaa tttcaatgcg ttgcccggtc
     6241 gcaatagcgt gacgcagccc ctgccagttc tccagccagg cttgcgcgac gacaggatca
     6301 tagtaacccg ccagcaacag tattgagtca atatcacgca tcaggcgtgg caactggtca
     6361 cggtagcgat cgcctaacgg ctggcagaaa acgcttttca gttcagcggc atggcgggaa
     6421 agatggatat cggcaaagcg tttgaaggag tcgctaattt tgccctgcgc tttggcatct
     6481 aaaaacggct gccatgcttt gcttaccagc cattcggtca acgccagctt cgccattgcc
     6541 gtttcggtag agtagaccgc cgtcacggca gaaaccgcag aagcgatggt cgcttcgcat
     6601 tgagtcagca aatcacgtaa gtgagtgctc gctttacgcg gcacaatgcc accgaacagc
     6661 atcagtgtat gacggactag gctaatggct gccagcacct gctctttcgc cgcatcgttg
     6721 ccgcgtaccc acagttcttc atgatactgc cattgcgcta acaccagctc gagcgccgct
     6781 tccagcccct gttccacatc ggcttttgcc gcaacatgca aaatggtggt cggtttgatt
     6841 tcacgcgccg gattgccctg cgccagatga tagccacgcg ccgctttgct caggctgccc
     6901 tggcgtaatc cggtttgcga taccagttgg ttcgccagtt tcagcaccgc gcgcgtgtcg
     6961 ccgctaagca gctccagttc cagctcgcag ataggctcag caaattcacc cgctttcact
     7021 tccccctggt cgagagcgat ttcaattcgg ctgccatcga tctctactag ccatttttcg
     7081 cgataaaaat cggtgctaaa cagcggctgc acgcgggaag cgagatcggc aggcaattcg
     7141 ccgttcggcc agatttccgt cggtaactgc gccaggtcga gcgtcggttc gctcaacgcc
     7201 acgttatatt ccgggcgctg gtgtaagccg cctgtcactc ttcctgcaac tttcatggtc
     7261 atctcatagc gaccgttttc gccacgaata cgtaagccca tatcgtgcct acgcagccag
     7321 ttatccggcg tttcgtagta aatattcagc aactgcacgg ggtcatggtg ctcgccgccc
     7381 agcgtattga gatggtcacg caacgcctca acggcactgt gattaacaat aaactttaat
     7441 tcgatttcct gagccatggc ctttacttat cggttatgtc acataagtga cgatgaacgg
     7501 cgaacttaat gcgatctttt ttgtcagtag atagtatttt gcgccaaatt gccatgcaac
     7561 gggcaatttg acgggcgtaa aagtttgaag cagtggcaag acctgacaca gaggattccg
     7621 attgatgtcg aatgctttgc gtagcgacac tgatgccact actatcgttc cactttccat
     7681 gacaataacg acagcctgat gccaaaatta cgcctgatcg gattaacttt actcgcactt
     7741 agcgcgactg ccgtctcaca cgctgaagaa acgcgctatg tttctgacga actgaatacc
     7801 tgggtccgta gcggtccggg agatcattat cgcctcgtgg gcacggttaa cgccggcgag
     7861 gaagtgacct tattacaaac tgacgccaac accaattatg cccaggtgaa agacagctct
     7921 ggccgtaccg cctggatccc gttgaaacaa cttagcactg agccaagcct gcgctcccgt
     7981 gtgccagatc tggaaaatca ggtcaaaacc ctgaccgata aactcaccaa tatcgataac
     8041 acctggaatc agcgcaccgc agaaatgcag caaaaagtgg cgcagagcga cagcgtgatc
     8101 aacgggttaa aagaagaaaa tcagaaactg aaaaacgagc tgattgtcgc gcagaaaaag
     8161 gtcgatgccg ccagcgtaca gctggatgac aaacagcgca ccatcatcat gcaatggttt
     8221 atgtatggcg gtggcgtgct ggggcttggc ttgctgctcg gtctggtact gccgcacctg
     8281 atcccaaacc gcaaacgcaa agatcgctgg atgaactaaa tcgccttctt cgttgcactg
     8341 tcttattatt aggaattgtg tatttgacga agggatgatg gcgtgaagat ttatctggtc
     8401 ggtggtgctg ttcgggatgc attgttaggg ctatcggtca aagacagaga ttgggtggtg
     8461 gtcggcagta cgccacagga gatgctcgac gcgggctacc agcaggtagg ccgcgatttt
     8521 cctgtgtttc tgcatccgca aacgcatgaa gagtatgcgc tggcacgtac cgaacggaaa
     8581 tccggttccg gttacaccgg ttttacttgc tatgccgcac cggatgtcac gctggaagat
     8641 gatcttaagc gtcgcgatct gaccattaat gcgctggccc aggacgataa cggcgagatt
     8701 atcgacccgt acaacggtct gggcgatctg caaaatcgtc tgttgcgcca tgtttccccc
     8761 gcttttggcg aagatccgtt acgcgtactg cgcgtggcgc gttttgctgc gcgttatgcc
     8821 cacctcggtt ttcgtattgc cgatgaaact ctgacgctga tgcgcgagat gacccatgcg
     8881 ggtgaactgg aacacctgac gcctgaacgg gtatggaaag agacggaaag cgccctcacc
     8941 acccgcaatc cccaggtgtt cttccaggta ctgcgcgatt gcggcgcact tcgcgtttta
     9001 ttcccggaaa ttgacgcact gtttggcgtt ccggcacctg ccaagtggca tccggaaatc
     9061 gacacgggta ttcatacctt aatgacgctc tcaatggcgg cgatgctgag tccgcaggtt
     9121 gatgtccgtt tcgcaacttt atgccacgat ctcggtaaag gactgacgcc gccagaactt
     9181 tggccgcgtc atcatgggca tggcccggcg ggtgttaagt tagtggaaca actatgccag
     9241 cgtctgcgcg tgccaaatga aattcgcgat ttagccagac tggtggctga gtttcacgat
     9301 ctcatccaca ccttcccaat gctgaaccca aaaaccatcg tcaaattatt tgattccatc
     9361 gatgcctggc gtaaaccgca gcgtgtcgag caactggcgc tgaccagcga ggctgacgtg
     9421 cgcggcagaa ccggttttga atcagcggac tacccgcaag gccgttggtt gcgcgaaggc
     9481 tgggaagtgg cgcagtcagt gccgacaaaa gcggtcgttg aagcgggatt taaaggcgtg
     9541 gagattcgcg aggagctgac ccgacggcgg attgcggcgg tagccagctg gaaggaacaa
     9601 cgttgcccaa agcctgactg agtataaaac taaacgttag cacgaaccgt cccctcgccc
     9661 cttcggggag agggttaggg tgaggggaac agaccagcac cggtgcgaac aatcaccccc
     9721 accccggccc tctccctaca gagcgaggga gaaaacccgg cctgctcccg aaccttttct
     9781 caatcaaaag aacacgacat acaccgcagc cgccacaata aagcgataaa tggcgaacgg
     9841 gataaacgaa atgcgcttaa tcaattgcag gaaggtttta atcgctatca gcgccaccac
     9901 aaaagcggtg ataaacccaa cggcaaacat cgggatatcg ccggttgtca ggaagcccca
     9961 gcttttgtag agatcgagcg ccgttgcgcc catcatcatc ggtaccgcca gcaggaacga
    10021 gaactcggaa gcagcgtaac ggctcacgcc catcagcatc ccacctgaaa tggtcgcccc
    10081 ggaacgggag aaacccggcc acagcgccag acactggaaa cagccaatca taaacgcctg
    10141 acgataggtc atatcatcaa gacccggcgc acgcggctct ttcggcttca ggcattcggc
    10201 ggcaatcagc aacaaaccgc caacgaccag cgcatacatc acatttatcg ggttaaacaa
    10261 tgacttaatt gtgtcgtgga ataatagccc cagtaccacc gccggaatca tccccagcaa
    10321 aatgtggatc agcgttaaac gacctttgct ttcaccttcg tgctgcaacg ggcggccaaa
    10381 gtggatgcca atcaggccaa acagacgccg ccagaacatc actactaccg ccagaattga
    10441 tcctaactgg atcacaactt caaaggtttt cgccgtgtcg ccctcaaatc ccaacaagtg
    10501 accgacaata atcatatggc ccgtgctgga taccggcaga aattctgtca atccttcgac
    10561 cacacccaat attgccgcta tcagcagcga gtgcatatcg ctcatcaata aacccctaaa
    10621 ttattaaaat gtaccgcttg tccgaactac tgcagtatga ccaggttata accgtttggt
    10681 ttaacagctg taaaattaat tattttcttt cagattattg ccacgctcaa tgattacgcc
    10741 aacattcgcc gcccgcgcca ctgcgcctgg cttgctgagt ttgatacgca cccacggcga
    10801 gttgaagcgt gctaacagca gctccgccac ctcttcagcc acgcgttcca ccagcgcaaa
    10861 acgcgccccc tcgacgtggc tgaccaccgt ttctgcaatg tcagcgtaac tgaggcaatc
    10921 cgccacatca tcacttttcg ccgctttacg gttatcccat gccatttcga tatcgaacac
    10981 taacttctgt tcgatggtct gttcccagtc gtaaacacca atagtggtga ttaccgaaag
    11041 ttgctctata aatacaatat ccatcacgtc ctgcctgctt tttggctaac cggataccac
    11101 ttccggcgaa atgtgcgtat tatccacaga ttcatcgttg aacacgaatt ttcaaaacgg
    11161 aacagcttat gagtgcaatc gcgcctggaa tgatcctcat cgcgtacctc tgcggctcca
    11221 tttccagtgc cattctggtt tgccgcttgt gtgggctgcc cgatccgcga accagcggct
    11281 ccggcaatcc aggcgcaacc aatgtgttac gtatcggtgg caagggagca gccgtagcag
    11341 tactgatttt cgacgttctg aaaggaatgt tgcctgtctg gggcgcgtat gaattaggtg
    11401 tcagcccctt ctggctaggc ttaattgcca tcgccgcctg tcttggacac atctggcccg
    11461 ttttcttcgg ttttaaagga ggaaaaggcg ttgctaccgc ttttggagca atcgcaccaa
    11521 ttggctggga tctcaccgga gtaatggcgg gaacctggtt actgaccgtg ctattgagcg
    11581 gatactcgtc gctgggagcg attgtcagtg cactgattgc tccgttttat gtctggtggt
    11641 ttaagccaca attcaccttc ccggtttcga tgctctcttg cctgatcctg ctgcgtcatc
    11701 atgacaacat ccagcgtctg tggcgtcgtc aggagacaaa aatctggacg aaattcaaaa
    11761 gaaagcgcga aaaggatccc gagtgatttc tggtggatct acatgacctg atagccttca
    11821 tcgggcttgc ccagccgttg ctggcaccat gccgccagaa attccacgca gacacgaatt
    11881 tcatgctgcg atagagcggc tcccggtaaa cagcccagat attggcgctt tgtgcatact
    11941 ccggcaatac ttgcaccagt ttgccactct ccagaaacgg caacacatcc cactcggaac
    12001 gcagcataat ccctttgccc tccagcgccc attgcaacac tatctcgccg ctattggagg
    12061 aaagatgccc gctaaccttc accgattttt tctcctgacc gttccccaac tcccatattc
    12121 cgtgggtcat atcgcgctct ttggtcacca gacagtcatg acgacttaat tcctgtaatg
    12181 attgtggctg cggatatttt tgcagatact caggtgctgc gcataatatt cttttatttt
    12241 tcgttaacag atgcgcaata taataatcag gaatttcgtc attaatacga atatccagat
    12301 caatattatc ctgcactaaa tcaatttgcc gatcgaacaa ttcaaaatgc acctgtaact
    12361 caggataatt gcgcataagt tcggtaatgg ctggcgcaat atggctgcgc ccaaaaccaa
    12421 aactgcaacc aatacgaatc atcccctccg ggcgcgtttt gatttgcgtg acgtcatcga
    12481 ccagtcgctg atattgggtg aggatttcca gcgcatgttc gtaacaacgt tggccacttt
    12541 cagtcagcgc cacgccgcga gcggagcggt tcagcagcgt tgttgccagc gtattttcaa
    12601 ggatttggat ccgcttggtg acaaatgcag gcgtttgccc aagcgtcgcc gctgcagcac
    12661 taaaactccc gctgtggacg atttctacca gtacctgcaa gtctttggct aaaggccagc
    12721 tattcagcat cgttaattat ccgcagttgt gataagcgca gtgtatttcg caaaacattg
    12781 ccattccctg ctctggcgca atattcattc ttattttcgc gttgattcac gaactgttaa
    12841 ttacatattc acggtggcaa aaaatataaa accacatttt tagtggtagt ttgtggcggt
    12901 gaatttttcc agacaaatac aaaaactgga gttgccatga tgagcgaaag taataagcaa
    12961 caggcagtga ataagttgac agagattgtc gctaacttta ccgccatgat ttctacccga
    13021 atgcctgatg acgtggtgga taaactaaaa cagctaaagg atgccgaaac gtcgtcgatg
    13081 gggaaaatta tctaccatac gatgttcgac aacatgcaaa aagcgattga cctgaatcgt
    13141 cctgcctgtc aggacaccgg ggagattatg ttcttcgtta aagtcggttc ccgcttccca
    13201 ctgcttggcg agctgcaaag catactcaaa caagccgtgg aagaggcaac cgtcaaagcg
    13261 ccactacgtc acaatgcggt agaaattttt gacgaagtaa acaccggcaa aaataccggt
    13321 agcggcgtac cgtgggtcac ctgggacatc atccccgaca atgacgatgc ggaaatcgaa
    13381 gtttacatgg caggcggcgg ctgcacgcta cctggccgct cgaaagtgtt aatgccgtca
    13441 gaaggctacg aaggcgtggt gaaattcgtc ttcgaaaata tctccaccct cgccgtaaac
    13501 gcctgtccac cggtactggt gggcgtgggc atcgccacct cggtggaaac cgccgccgta
    13561 ctctcgcgta aagccattct gcgcccgatt ggcagccgcc atcccaatcc aaaagcggca
    13621 gaactggagc tacgcctgga agaaggactc aaccgtctgg ggattggtcc acaagggctg
    13681 accggcaaca gttcagtgat gggcgcatcg tcgcggcacg ctgctggttc atgccgatct
    13741 cacctttgaa aatctgtctc acacccggag cgcgttatga aaaagatcct gacaacctcg
    13801 atcaaagctg aagatctgca agatattcgc gtcggcgatg tgatctacct gaccggtacg
    13861 ctggtgacct gccgcgacgt ttgtcaccgc cgtttgatcg aactgaaacg tccgatccct
    13921 tacgatctca acggcaaagc gattttccac gctggcccca tcgtgcgcaa aaacggcgac
    13981 aaatgggaga tggtctccgt cggcccgaca accagtatgc gtatggaaag ttttgaacgt
    14041 gaatttattg agcagaccgg cgtaaaactg gtggttggca aaggtggtat ggggccgctg
    14101 accgaagaag gctgccagaa attcaaggcg ttacatgtga ttttcccggc aggctgcgcg
    14161 gtgctggcgg caacccaggt agaagagatt gaagaagtgc actggacaga actcgggatg
    14221 ccggagtcac tgtgggtctg ccgggtcaaa gagttcggcc cgctgattgt ctctattgat
    14281 acccacggca acaacctgat agccgaaaac aaaaagctgt tcgccgaacg ccgcgatccc
    14341 atcgtggaag agatctgcga gcacgtccat tacatcaaat aaccctcccg gagaggctca
    14401 cccctctcct ttttcgcagg cataacacga tgaaaccttc cactgaatgg tggcgatatc
    14461 ttgcaccgct ggcggtcatc gccattattg ctctacttcc ggttcccgca gggctggaga
    14521 accatacctg gctctacttt gccgttttta ctggcgtgat cgttggactg atcctcgaac
    14581 ccgtgccggg tgccgtggtg gcgatggtgg gtatctccat catcgccata ctctctccct
    14641 ggctgctgtt cagccccgaa cagctcgccc agccaggctt taagttcacc gccaaatccc
    14701 tctcctgggc agtttctggt ttttctaatt cggttatctg gctcattttc gccgccttta
    14761 tgtttggcac aggctatgaa aaaaccgggc ttggacgccg cattgcgctg attctggtga
    14821 aaaagatggg gcatcgcacg ctgtttctcg gctatgcggt gatgttctcc gagctgatcc
    14881 tagcacctgt aacaccgtcc aactcggcgc gtggtgcggg tattatctat cccatcatcc
    14941 gtaacctgcc accgctctat caatcacaac caaacgacag cagttcgcgc agcattggct
    15001 cgtacatcat gtggatgggg attgttgccg actgcgtgac cagcgccatt ttcctgacgg
    15061 cgatggcacc taacttgctg ttaattggac tgatgaaaag cgcatctcac gccacgctga
    15121 gttggggcga ctggttcctc gggatgttgc cgctcagcat tttactgctt ctgctggttc
    15181 cctggctggc ttacgtgcta tacccgccgg tactgaagtc tggtgatcag gtgccgcgct
    15241 gggcagagac ggaactgcag gcaatgggcc cgctctgttc gcgtgaaaaa cggatgctgg
    15301 ggctgatggt aggcgcgctg gtgctgtgga ttttcggcgg tgattatatc gatgccgcga
    15361 tggtcggtta cagcgtggtg gcgctgatgc tgcttctgcg cattatcagt tgggacgaca
    15421 ttgtcagtaa taaagcggca tggaacgttt tcttctggct ggcctcgctt atcaccctcg
    15481 cgaccggact caacaatacc ggttttatta gctggtttgg caaactgtta gcaggcagct
    15541 taagtggtta tccgccgacg atggtgatgg tgacgttgat tgtggtgttt tatctactgc
    15601 gctacttttt cgccagcgcc acggcgtata cctccgccct cgcaccgatg atgatcgccg
    15661 ccgcgctggc gatgccggaa atcccgctgc cggtgttctg cctgatggtt ggcgcggcaa
    15721 ttggtctggg gagcattctt acaccatacg ccaccggacc cagtccgatt tactacggta
    15781 gtggttatct gccaacggcg gattactggc gactgggggc gatttttggg ctgatattcc
    15841 tcgtgttgct ggtgattacc ggcttactgt ggatgcccgt ggtgttgctt taagtgagga
    15901 cggtgccgga aattgctctc cggcacccaa tagttttacg cagccggtaa ctccgccagc
    15961 ggccagcgcg gacgcacgct aacgccgaga tccgccgtcg cgcctgcttt aaaccgcacc
    16021 attccggcat aggcgatcat cgcgccgtta tcagtacaaa attccggacg cgcgtagaac
    16081 acttcgccgc ggcgtttttt catcatttca gccagcttcg cccgtaacgt gcggttagca
    16141 ctcacgccgc ccgccatgac cagtcgctta aagcccgtct gatccaacgc tcgcttacac
    16201 ttaatcatca acgtatcgac caccgcatct tcaaaggcgc gggcgatatc agcacgcgtc
    16261 tggtcgtcgg tgctgttgtc acgaatcgtg ttcgccgcaa aggttttcag accagaaaag
    16321 ctgaaatcca gccccggacg gtcggtcatc ggacgcggga aaacaaagcg cccggcagta
    16381 ccctgagccg ccattttcga cagtaacggt ccgccaggat aatccagccc cagcagcttc
    16441 gcggttttat caaacgcttc accggcggca tcatcgatag actcgccgag cagctcgtac
    16501 tgaccaatgc cagtcacgct gattaactgc gtatgaccgc cggacaccag cagcgcgaca
    16561 aacggaaatt ccggcgggtt atcttccagc atcggcgcta acagatgccc ttccatatgg
    16621 tgtaccggga ttgccggaac gttccaggca aacgccagag agcgccccac ggttgcgcca
    16681 accagcagtg cgccgactaa tccagggcct gcggtatagg ccacagcatc aatgtctttt
    16741 gccgttaaac cagactcctt tagcgccgcc tggatcaacg gtacggtttt acgcacatga
    16801 tcgcgggagg ccagttcagg cacgacgccg ccgtagtcag cgtgcaattt cacctgacta
    16861 tacaattggt tggctaacaa acctttttca tcgtcgtaaa tggcgatgcc ggtttcatcg
    16921 caggaagttt caatacccag tacacgcatg acttgtttta cctcgcttta ttaccgcgca
    16981 gtgtaggacc aatgcgggtt gatgtaaaac tttgttcgcc cctggagaaa gcctcgtgta
    17041 tactcctcac ccttataaaa gtccctttca aaaaaggccg cggtgcttta caaagcagca
    17101 gcaattgcag taaaattccg caccattttg aaataagctg gcgttgatgc cagcggcaaa
    17161 ccgaattaat caaaggtgag aggcacatgc cggtaattaa agtacgtgaa aacgagccgt
    17221 tcgacgtagc tctgcgtcgc ttcaagcgtt cctgcgaaaa agcaggtgtt ctggcggaag
    17281 ttcgtcgtcg tgagttctat gaaaaaccga ctaccgaacg taagcgcgct aaagcttctg
    17341 cagtgaaacg tcacgcgaag aaactggctc gcgaaaacgc acgccgcact cgtctgtact
    17401 aattccccga gagcgttgct ctccgatcag accgagttgt agttgtaagg ccgtgcttcc
    17461 gaaaggaatg cgcggcttat tttcgtttat gaattgctaa aaatcggggc ctatggctgg
    17521 acgaatccca cgcgtattca ttaatgatct gctggcacgc actgacatcg tcgatctgat
    17581 cgatgcccgt gtgaagctga aaaagcaggg caagaatttc cacgcgtgtt gtccattcca
    17641 caacgagaaa accccgtcat tcaccgttaa cggtgagaaa cagttttacc actgctttgg
    17701 atgtggcgcg cacggcaacg cgatcgactt cctgatgaac tacgacaagc tcgagttcgt
    17761 cgaaacggtc gaagagctgg cagcaatgca caatcttgaa gtgccatttg aagcaggcag
    17821 cggccccagc cagatcgagc gccatcagag gcaaacgctt tatcagttga tggacggtct
    17881 gaatacgttt taccaacaat ctttacaaca acctgttgcc acgtctgcgc gccagtatct
    17941 ggaaaaacgc ggattaagcc acgaggttat cgctcgcttt gcgattggtt ttgcgccccc
    18001 cggctgggac aacgtcctga agcggtttgg cggcaatcca gaaaatcgcc agtcattgat
    18061 tgatgcgggg atgttggtca ctaacgatca gggacgcagt tacgatcgtt tccgcgaacg
    18121 ggtgatattc cccattcgcg ataaacgcgg tcgggtgatt ggttttggcg ggcgcgtgct
    18181 gggcaacgat acccccaaat acctgaactc gccggaaaca gacattttcc ataaaggccg
    18241 ccagctttac ggtctttatg aagcgcagca ggataacgct gaacccaatc gtctgcttgt
    18301 ggtcgaaggc tatatggacg tggtggcgct ggcgcaatac ggcattaatt acgccgttgc
    18361 gtcgttaggt acgtcaacca ccgccgatca catacaactg ttgttccgcg cgaccaacaa
    18421 tgtcatttgc tgttatgacg gcgaccgtgc aggccgcgat gccgcatggc gagcgctgga
    18481 aacggcgctg ccttacatga cagacggccg tcagctacgc tttatgtttt tgccggatgg
    18541 cgaagaccct gacacgctgg tacgaaaaga aggtaaagaa gcgtttgaag cgcggatgga
    18601 gcaggcgatg ccactctccg catttctgtt taacagtctg atgccgcaag ttgatctgag
    18661 tacccctgac gggcgcgcac gtttgagtac gctggcactg ccattgatat cgcaagtgcc
    18721 gggcgaaacg ctgcgaatat atcttcgtca ggaattaggc aacaaattag gcatacttga
    18781 tgacagccag cttgaacgat taatgccaaa agcggcagag agcggcgttt ctcgccctgt
    18841 tccgcagcta aaacgcacga ccatgcgtat acttataggg ttgctggtgc aaaatccaga
    18901 attagcgacg ttggtcccgc cgcttgagaa tctggatgaa aataagctcc ctggacttgg
    18961 cttattcaga gaactggtca acacttgtct ctcccagcca ggtctgacct ccgggcaact
    19021 tttagagcac tatcgtggta caaataatgc tgccaccctt gaaaaactgt cgatgtggga
    19081 cgatatagca gataagaata ttgctgagca aaccttcacc gactcactca accatatgtt
    19141 tgattcgctg cttgaactgc gccaggaaga gttaatcgct cgtgagcgca cgcatggttt
    19201 aagcaacgaa gaacgcctgg agctctggac attaaaccag gagctggcga aaaagtgatt
    19261 taacggctta agtgccgaag agcgatcggg aagcccccga cagccgcact gagaggcagc
    19321 ggcaaatata taagtacgcc ctcgtaatta tcgttggcgg taaacaaccg ttggatttca
    19381 gcgttaacgg ctgaaggaca tcgggtcaat cgcccaacac caacctcatg aaataagtgt
    19441 ggataccgtc ttatggagca aaacccgcag tcacagctga aacttcttgt cacccgtggt
    19501 aaggagcaag gctatctgac ctatgccgag gtcaatgacc atctgccgga agatatcgtc
    19561 gattcagatc agatcgaaga catcatccaa atgatcaacg acatgggcat tcaggtgatg
    19621 gaagaagcac cggatgccga tgatctgatg ctggctgaaa acaccgcgga cgaagatgct
    19681 gccgaagccg ccgcgcaggt gctttccagc gtggaatctg aaatcgggcg cacgactgac
    19741 ccggtacgca tgtacatgcg tgaaatgggc accgttgaac tgttgacccg cgaaggcgaa
    19801 attgacatcg ctaagcgtat tgaagacggg atcaaccaag ttcaatgctc cgttgctgaa
    19861 tatccggaag cgatcaccta tctgctggaa cagtacgatc gtgttgaagc agaagaagcg
    19921 cgtctgtccg atctgatcac cggctttgtt gacccgaacg cagaagaaga tctggcacct
    19981 accgccactc acgtcggttc tgagctttcc caggaagatc tggacgatga cgaagatgaa
    20041 gacgaagaag atggcgatga cgacagcgcc gatgatgaca acagcatcga cccggaactg
    20101 gctcgcgaaa aatttgcgga actacgcgct cagtacgttg taacgcgtga caccatcaaa
    20161 gcgaaaggtc gcagtcacgc tgccgctcag gaagagatcc tgaaactgtc tgaagtattc
    20221 aaacagttcc gcctggtgcc gaagcagttt gactacctgg tcaacagcat gcgcgtcatg
    20281 atggaccgcg ttcgtacgca agaacgtctg atcatgaagc tctgcgttga gcagtgcaaa
    20341 atgccgaaga aaaacttcat taccctgttt accggcaacg aaaccagcga tacctggttc
    20401 aacgcggcaa ttgcgatgaa caagccgtgg tcggaaaaac tgcacgatgt ctctgaagaa
    20461 gtgcatcgcg ccctgcaaaa actgcagcag attgaagaag aaaccggcct gaccatcgag
    20521 caggttaaag atatcaaccg tcgtatgtcc atcggtgaag cgaaagcccg ccgtgcgaag
    20581 aaagagatgg ttgaagcgaa cttacgtctg gttatttcta tcgccaagaa atacaccaac
    20641 cgtggcttgc agttccttga cctgattcag gaaggtaaca tcggcctgat gaaagcggtt
    20701 gataaattcg aataccgccg tggttacaag ttctccacct acgcaacctg gtggatccgt
    20761 caggcgatca cccgttctat cgcggatcag gcgcgcacca tccgtattcc ggtgcatatg
    20821 attgagacta tcaacaaact caaccgtatt tctcgccaga tgctgcaaga gatgggccgt
    20881 gaaccgacgc cggaagaact ggctgaacgt atgctgatgc cggaagacaa gatccgcaaa
    20941 gtgctgaaga tcgccaaaga gccaatctcc atggaaacgc cgatcggtga tgatgaagat
    21001 tcgcatctgg gggatttcat cgaggatacc accctcgagc tgccgctgga ttctgcgacc
    21061 accgaaagcc tgcgtgcggc aacgcacgac gtgctggctg gcctgaccgc gcgtgaagca
    21121 aaagttctgc gtatgcgttt cggtatcgat atgaacaccg accacacgct ggaagaagtg
    21181 ggtaaacagt tcgacgttac ccgcgaacgt atccgtcaga tcgaagcgaa ggcgctgcgc
    21241 aaactgcgtc acccgagccg ttctgaagtg ttgcgtagct tcctggacga ttaatcggta
    21301 ggccggatca ggcgttacgc cgcacccggc actagtctct ctgcacaaac gccaccttt
//
LOCUS       DADMEQ010000061        21217 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.061, whole genome shotgun sequence.
ACCESSION   DADMEQ010000061 DADMEQ010000000
VERSION     DADMEQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21217
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.061"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(32..1012)
                     /locus_tag="JNZ05_002334"
     CDS             complement(32..1012)
                     /locus_tag="JNZ05_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HAY8565546.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSALDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1020..1730)
                     /locus_tag="JNZ05_002335"
     CDS             complement(1020..1730)
                     /locus_tag="JNZ05_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HAY8565547.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(1947..3770)
                     /gene="typA"
                     /locus_tag="JNZ05_002336"
     CDS             complement(1947..3770)
                     /gene="typA"
                     /locus_tag="JNZ05_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HAY8565548.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4143..5552
                     /gene="glnA"
                     /locus_tag="JNZ05_002337"
     CDS             4143..5552
                     /gene="glnA"
                     /locus_tag="JNZ05_002337"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HAY8565549.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5725..6774
                     /gene="glnL"
                     /locus_tag="JNZ05_002338"
     CDS             5725..6774
                     /gene="glnL"
                     /locus_tag="JNZ05_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HAY8565550.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6786..8195
                     /gene="glnG"
                     /locus_tag="JNZ05_002339"
     CDS             6786..8195
                     /gene="glnG"
                     /locus_tag="JNZ05_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HAY8565551.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8307..8417
                     /gene="yshB"
                     /locus_tag="JNZ05_002340"
     CDS             8307..8417
                     /gene="yshB"
                     /locus_tag="JNZ05_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HAY8565552.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8646..10019)
                     /gene="hemN"
                     /locus_tag="JNZ05_002341"
     CDS             complement(8646..10019)
                     /gene="hemN"
                     /locus_tag="JNZ05_002341"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAY8565553.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10208..10717)
                     /gene="yihI"
                     /locus_tag="JNZ05_002342"
     CDS             complement(10208..10717)
                     /gene="yihI"
                     /locus_tag="JNZ05_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HAY8565554.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11299..11931
                     /locus_tag="JNZ05_002343"
     CDS             11299..11931
                     /locus_tag="JNZ05_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAY8565555.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12313..15099)
                     /gene="polA"
                     /locus_tag="JNZ05_002344"
     CDS             complement(12313..15099)
                     /gene="polA"
                     /locus_tag="JNZ05_002344"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAY8565556.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15101..15343
                     /locus_tag="JNZ05_002345"
     CDS             15101..15343
                     /locus_tag="JNZ05_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565557.1"
                     /translation="MSVPVYQIMGKGCHRQDENMSFSPLFVKIFCRSAGSLANRHVDN
                     FVHKLFTSLLIYYHAFYSMMFVFIFHAVKNKSIPNN"
     gene            15463..16395
                     /locus_tag="JNZ05_002346"
     CDS             15463..16395
                     /locus_tag="JNZ05_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8565558.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLISSQRQNK"
     gene            complement(16436..17866)
                     /locus_tag="JNZ05_002347"
     CDS             complement(16436..17866)
                     /locus_tag="JNZ05_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY8565559.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRIQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNVVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(18021..18647)
                     /gene="dsbA"
                     /locus_tag="JNZ05_002348"
     CDS             complement(18021..18647)
                     /gene="dsbA"
                     /locus_tag="JNZ05_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HAY8565560.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(18664..19650)
                     /gene="srkA"
                     /locus_tag="JNZ05_002349"
     CDS             complement(18664..19650)
                     /gene="srkA"
                     /locus_tag="JNZ05_002349"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HAY8565561.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQESPLQLTPMY"
     gene            complement(19727..19996)
                     /locus_tag="JNZ05_002350"
     CDS             complement(19727..19996)
                     /locus_tag="JNZ05_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HAY8565562.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20066..20650
                     /gene="mobA"
                     /locus_tag="JNZ05_002351"
     CDS             20066..20650
                     /gene="mobA"
                     /locus_tag="JNZ05_002351"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HAY8565563.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20632..21159
                     /gene="mobB"
                     /locus_tag="JNZ05_002352"
     CDS             20632..21159
                     /gene="mobB"
                     /locus_tag="JNZ05_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HAY8565564.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDAAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5173 a         5340 c         5113 g         5591 t
ORIGIN      
        1 gttttattaa ctgcgtatga gttaaaactt attagtggtt cagaagatgg tttgcctctt
       61 gtttgatttg ttgtagtacg ttacgcacat gatttatctg attaattgca tcttcttttt
      121 ctttacgtaa ccgcgcatcc agtaaatgat tttctggtag gggtgtttca ctcatctggc
      181 gataagggat ccacggatta tcatcttcgt cttcaaagcg gaacgccggc gcatagtaat
      241 caacctcttc ttccaggcca tatgccgtca cctgcggctc atcatcacca aggcagataa
      301 ggtgcgttaa taacgctttg aatggcatat caccctgacc tgaaatacag gctttatgac
      361 ccaggccacc ctgttcttta acgatcaagg catctttaat atggacctgg gtaatatgcg
      421 gtgccatcgt ttttaaggcg tcgatgggat gctcatttgc attgatcatg ttagcaaaat
      481 caaacagtaa ggaaagagac tccatctcac tttctttgac cagcgacacc agctcatgac
      541 tttttaaatc ttcatgctgc tcaatcgtaa aagtcaggcc gctgtcctga tacgtttccc
      601 gtacataggc aatgtcgtta gcgataatcg ataatacgtc gcgcaaatta ccttcataac
      661 gtggataaaa acgtacggac gttgccccag ttttcaacgc gatggcgacg gcttcgtcaa
      721 tagatgcctt atctaaagcg ctggtttcaa tatgaatatc aaggttcaga cggcgggctt
      781 tatccccaaa ggcagagagt tctttatcgt ccatattacc aagagaaaaa cgctcgccat
      841 ccagaacatg gattttgacg ccacgtagat tattttcact tgcgatatca agtaaatcag
      901 caggcaacac tctttccagc cgcatattta aatgaaaggc ataagcatgt aaataaagag
      961 gaagagtgtc gacacgttgt agaatctttc ttgcgttatt tattgtcacc ataaaacacc
     1021 tacagattaa tgatgaggtc tacggcctca aatcgccaat attcaatatc tatctggaca
     1081 atattgcctt gctggtcggc gcgatgcttt tcaattaaga tcgctggcat acccgcagaa
     1141 ccgccaagat acttgctgat atcgcccggc attctgacgg gtttgaaaat caattttttt
     1201 actaccgtct ctttttggta gcacttttcc cagactgcag aaaatgagtg gttttcaagt
     1261 tgttcaataa aacccggagc aacttcagga ttaatatatg tttcgtgata gaaaacttta
     1321 tgtccttcca gcgcccccca gccggtgatg cgatagagtt cagttgatgg cgtcactgca
     1381 atcagtggcg cgagcgtctc gggaatatcg ctggtacggt ttttctcggt aaacccccaa
     1441 gaaggctctc gcccttgctc aattgcggcc cgctgaaagc tcgccgacag ctccggactg
     1501 taattaaatc gcggctgggt cacgaaccac cccttacgat ccttacggaa gattttggat
     1561 tctgcctcga ggttcaacaa cgcctggcgc agcgtcatac gtttaatgcc cagcagttct
     1621 cccagctctc tttctgaagg gagttttcca cccggcgtgg tgatgccatc tttcaaccac
     1681 cgatccagtt tctcttttgc attttctacg gtggattgat tctccgccat tgtttaatcc
     1741 ttttggttta aaccagatga agcatcttac gcttacgcca aaaaagaaag caattaacgg
     1801 tgtatgactt tgcagataat tgtgacaaag ctctctattg acgtaatcac agacttataa
     1861 gggagatact cgcattggtg tgcgctcacg acaggtaaaa aaaaacctgc catcgctggc
     1921 aggtttttta tgactaaaaa acgaaattaa tcgtctttcg gtgcgcggtt ggcgcggcga
     1981 cgatcgtttt ccgtcaggtg acgtttacga atacggatag aggtcggagt cacttctacc
     2041 agttcgtcat catcgatgaa ctccagagct tgttccagag tcatgcggat aggcggaacc
     2101 agaacaacag cttcgtcagt accggaagca cgcatgttgg tcagtttctt accggtcagg
     2161 cagtttacag tcaggtcgtt agagcggcta tgaataccga taatctgacc ttcgtaaact
     2221 tctgcaccgt gaccgaggaa cagcttaccg cgatcctgca gaccgaacag cgcgaacgcg
     2281 acagctttac cctgaccgtt agagatcagt acaccgttct gacgctgacc cacttcgccc
     2341 ggacgtacgt cgtcgtagtg gctgaaggtg gagtacagca gaccagtacc ggaagtcatg
     2401 gtcatgaact cagaacggaa gccaatcaga ccacggcttg ggatcacgta gtcgagacgt
     2461 acgcggcctt taccgtctgg attcatgttt ttcaggtcgc ctttacgttc gcccagcgcc
     2521 tgcatcacag aaccctgatg ctgttcttca acgtccagcg tcacgttttc atacggctct
     2581 tgtttacgac cgtcgatttc acggaagatt actttcggac gggataccgc cagttcgaaa
     2641 ccttcacgac gcatgttttc gataagaaca gagaggtgca gttcaccacg accagaaacg
     2701 cggaacgcat cggcgtcttc ggtttcttct acgcgcagcg caacgttgtg taccagttct
     2761 ttgttcagac gatcgaggat ctgacgagac gttacgaact taccttcttt accgcagaac
     2821 ggcgaggtgt taacgcagaa gaacatagaa acggtcggct catcaacgga gagtgccggc
     2881 agcgcttcaa cgttttgcgt gtcgcaaacg gtgtcagaaa tgttcagttc gcccaggccc
     2941 gtgatcgcca cgatatcgcc agcttccgcc agatcggttt cgatacgttc cagaccgagg
     3001 tggcccagca ctttaccgac tttcgcgtta cgggttttgc cttcgctatc gatgatagta
     3061 acctgctggt tcggcttcac tttaccacgc ttgatgcggc caatgccgat aacgccaaca
     3121 tagctgttgt aatcgagctg agaaatctgc atctggaacg gaccgtcaag gtcaacgtcc
     3181 ggcgcaggaa cgtggtcaac aatcgcctga tacagcgggg tcatgtcttc cgccatatct
     3241 tcgtggtcca gacccgcgat accgttcagc gcagaagcgt aaacgatcgg gaagtccagc
     3301 tgctcgtcgg tcgcgtcgag gttaacgaac agatcgaata cctgatccac aacccaatca
     3361 ggacgcgcgc cagggcggtc aactttgttg ataacaacaa tcggcttcag gccgtaagca
     3421 aacgcttttt tggttacgaa gcgcgtttgc ggcatcgggc cgtcaaatgc gtcaaccacc
     3481 agcagcactg agtctaccat ggacattaca cgttcaactt caccaccgaa gtcggcgtgc
     3541 cccggggtat caacgatgtt gatacggtaa tcattccatt tgatagcggt gtttttcgcg
     3601 aggatggtaa tcccacgctc tttctccaaa tcgttggagt ccatcacgcg ctcttgggtt
     3661 tcggcacgag agtcgaacgt accggattgt tggagcagct tgtctaccaa ggtggtttta
     3721 ccatggtcta cgtgcgcgat gatggcgata ttacgcaatt tttcgatcac aactttgcct
     3781 caggcattag aaatagcgcg ttattgtaca cggattaatc gcactacaaa acaggatcac
     3841 aaacatcctc cgcaaacaag tattgcagag tccctttgtg atcgctttca cggagcataa
     3901 aaagggttat ccaaaggtca ttgcaccaat atggtgctta atgtttccat tgaagcacta
     3961 tattggtgca acattcacat catggtgcag cccttttgca cgatgttaag catgataacg
     4021 ccttttaggg gcaatttaaa agttggcaca gatttcgctt tatctttttt acggcgacac
     4081 ggccaaaata attgcagatt tcgttaccac gacgacaatg accaatccag gagagttaaa
     4141 gtatgtccgc tgaacacgta ctgacgatgc tgaacgagca cgaagtgaag tttgttgatt
     4201 tgcgcttcac cgataccaaa ggtaaagaac aacacgtcac tatccctgct catcaggtga
     4261 atgctgaatt cttcgaagaa ggcaaaatgt ttgacggctc ctcgattggc ggctggaaag
     4321 gcattaacga atccgacatg gtgctgatgc cagacgcatc caccgcagtg attgacccgt
     4381 tcttcgccga ctccaccctg attatccgtt gcgacatcct tgaacctggc accctgcaag
     4441 gctatgaccg tgacccgcgc tctattgcga agcgcgccga agattacctg cgttccactg
     4501 gcattgccga caccgtactg ttcgggccag aacctgaatt cttcctgttc gatgacatcc
     4561 gtttcggatc atctatctcc ggttcccacg ttgctatcga cgatatcgaa ggcgcatgga
     4621 actcctccac ccaatacgaa ggtggtaaca aaggtcaccg tccggcagtg aaaggcggtt
     4681 acttcccggt tccgccggta gactctgctc aggatattcg ttctgaaatg tgtctggtga
     4741 tggaacagat gggtctggtg gttgaagccc atcaccacga agtagcgact gctggtcaga
     4801 acgaagtggc tacccgcttc aataccatga ccaaaaaagc tgacgaaatt cagatctaca
     4861 aatatgtcgt gcataacgta gcgcaccgct tcggtaaaac cgcgaccttt atgccaaaac
     4921 cgatgttcgg tgataacggc tccggtatgc actgccacat gtctctgtct aaaaacggcg
     4981 ttaacctgtt cgcaggcgac aaatacgcag gtctgtctga gcaggcgctg tactacattg
     5041 gcggcgtaat caaacacgct aaagcgatta acgccctggc aaacccgacc accaactctt
     5101 ataagcgtct ggtcccgggc tacgaagcac cggtaatgct ggcttactct gcgcgtaacc
     5161 gttctgcgtc tatccgtatt ccggtggttt cttctccgaa agcacgtcgt atcgaagtac
     5221 gtttcccgga tccggcagct aacccgtacc tgtgctttgc tgccctgctg atggccggtc
     5281 ttgatggtat caagaacaag atccatccgg gcgaagccat ggacaaaaac ctgtatgacc
     5341 tgccgccaga agaagcgaaa gagatcccac aggttgcagg ctctctggaa gaagcactga
     5401 acgaactgga tctggaccgc gagttcctga aagccggtgg cgtgttcacg gatgaagcga
     5461 tcgatgcgta catcgctctg cgtcgcgaag aagatgaccg cgtgcgtatg actccgcatc
     5521 cggtagagtt tgagctgtac tacagcgtct aatagttgaa gttgtactac ccggcgcaca
     5581 acgccgggat ttagttgccg tggaaacttt cagcccatct ctgcatgggc ttttttctcc
     5641 gtcaattctc tgatgcttcg cgctttttat ccgtaaaaag ctataatgca ctaaaatggt
     5701 gcaacctgtt caggagactg ctttatggca acaggcacgc agcccgatgc tgggcagatc
     5761 ctcaactcgc tgattaacag tattttgtta attgatgaca atctggcgat ccattacgcc
     5821 aaccccgccg cgcaacaact gctcgcccaa agctcccgca aattgtttgg tacgccgtta
     5881 ccggaactgt tgagctactt ctcattaaat atcgagctga tgcaagaaag tctggaggcg
     5941 gggcaaggct ttaccgataa cgaagtgacg ctggtcatcg atggtcgctc gcatatcctt
     6001 tctgtgacgg cccagcgtat gccggacggc atgatcctgc tggagatggc accgatggat
     6061 aaccagcgcc gcttaagcca ggaacaacta caacatgctc aacaagtagc tgcccgtgat
     6121 ttagtgcgcg gcctggcaca tgagattaaa aatccgcttg gcggtttacg tggcgcggcg
     6181 cagttactca gcaaagcgtt acctgaccca tcactactcg aatataccaa agtgattatc
     6241 gaacaggcgg accggctgcg aaatttggtc gaccgtctgt tggggccgca gctgcccggt
     6301 acgcgcgtta ccgaaagtat tcacaaagtg gctgaacgcg tggtaacgct ggtgtcgatg
     6361 gaactgccgg acaacgtgcg gttgattcgt gattacgatc ccagcctgcc ggaactggcg
     6421 cacgacccgg atcaaattga acaggtcttg ctgaatattg tgcgcaatgc gctacaggcg
     6481 ctggggccgg aaggcggtga aatcattctg cgtacccgca ccgcgtttca actgacctta
     6541 cacggcgagc gctaccggct ggcggcgcgg attgatgtgg aagataacgg gccgggcatt
     6601 ccgcctcatt tgcaggatac gctgttttac ccgatggtca gcggccgcga aggtggcacc
     6661 gggcttggct tatccatcgc ccgtaatttg attgatcagc attcaggcaa aattgaattt
     6721 accagttggc caggtcatac cgagttctcg gtttacctgc ctatcaggaa ataaaggtga
     6781 cgtttatgca acgagggata gtctgggtag tcgatgacga tagttccatc cgttgggtgc
     6841 ttgaacgtgc gctcgctgga gcgggtttaa cctgtacgac atttgagaac ggcgcggaag
     6901 tactggaggc gctggcgagc aaaacgccgg atgtgctgct ttcagatatc cgtatgccgg
     6961 gaatggacgg gctggcgctg ctcaagcaga ttaaacagcg ccatccaatg cttccggtca
     7021 tcattatgac cgcacattcc gatctggatg ctgccgtcag cgcctatcaa caaggggcgt
     7081 ttgattatct gcccaaaccg tttgatatcg acgaagcagt ggcgctggtt gagcgcgcta
     7141 tcagtcatta ccaggaacag cagcagccgc gtaatattca gcttaacggc ccaacgaccg
     7201 atatcatcgg cgaagcgcaa gccatgcagg acgtgttccg tattatcggt cggctttcgc
     7261 gttcttctat tagcgtgctg attaacggcg aatccggcac cggtaaagaa ctggtcgctc
     7321 atgccctgca tcgccacagt ccgcgcacca aagcgccgtt tatcgcgctg aatatggcag
     7381 ctatcccaaa agatttgatc gaatcagaac tgtttggcca cgagaaaggc gcgtttactg
     7441 gcgcgaatac cattcgtcag gggcgttttg aacaggccga tggcggcacg ttatttctcg
     7501 acgaaattgg agatatgccg ctggatgtgc agacgcgttt gctgcgcgtg ctggcagacg
     7561 gtcagtttta tcgcgttggc ggctatgcgc cggtgaaagt ggatgtgcgg attatcgctg
     7621 ccactcacca gaatcttgaa cagcgggtgc aggaaggtaa gttccgtgag gatctgttcc
     7681 accgcctgaa cgttatccgc gttcatctgc cgccgctgcg cgaacgtcgg gaagatattc
     7741 cccgtctggc gcgccatttt ttacaggttg ccgcgcgcga actgggcgta gaagcgaagt
     7801 tgctgcatcc ggaaaccgaa gctgctctga cgcgtctggc gtggccaggc aacgtgcgcc
     7861 agctggaaaa cacctgccgc tggctaacgg tgatggccgc cgggcaggaa gtgttgattc
     7921 aggatttgcc cggcgaactg tttgaatcaa cggttgcgga gagtacttcg caaatgcaac
     7981 cggacagttg ggcgacactt ttagcgcagt gggcagacag agcgctgcgt tccggtcatc
     8041 aaaatctgct ttccgaagcg cagccagagc tggagcggac gttactgacg accgcgttgc
     8101 gacatacgca ggggcataaa caggaagcgg cgcggctact cggctggggc cgcaacactc
     8161 tgacgcgtaa gttaaaagag ctggggatgg agtgattcac ggcttgtgtg taaagattga
     8221 ttattgagcg caaattgctg gtattttgcg ctttactgtt ccgataagtt cagtatgatc
     8281 ttgcccggaa aacggggaga gtcattatgc tggaatcaat aattaatctg gtatcgagtg
     8341 gcgcagttga cagccacaca ccgcaaactg ccgttgctgc cgtgctgtgt gccgcgatga
     8401 ttgggctgtt tagctgaagc tcgttgttta tgccggatgc ggagaaagtc gtaggcctga
     8461 taagacgcga ccagcgtcgc atcaggcatc atgctcagat gcctgatgcg gcgtaaacgc
     8521 cttatccggc ctacaaaagc aataatttca acaggttttg ttgcccagcg catcaggcaa
     8581 tcttgttctc agcacattga tggcgctttc gttttctact ttgtaaacga agcgccattc
     8641 actctttaaa tcacccgtga gaactgctgc atccgcgctt tctggcgcag ataggtatca
     8701 aagcacatgc aaatgttgcg gatcagcaag cgacctttcg ccgtcacctg tattcccttc
     8761 tcatccacat ccaccagccc atcttttgct aacggggcga gcagcttgag atcttccgca
     8821 aagtaatcag cgaagtgcaa atcccactgt ttctcaagag gggcgtaatc cagacggaag
     8881 ttgcagatga gcgacttaat cacatcgcgg cgaatacagt catcacgcgt tagcgcaata
     8941 ccacgccaca gcgcattgcc ttgttcatcc acttgctgat agtactgctt caactctttc
     9001 tggttctgcg cgtagcagtc gccaatcatg ctgatggcgg aaacgcccat ccccagcaga
     9061 tcggtatcgc cctgagtggt gtagccctgg aagttacgat gcagcacgcc ttcacgctgg
     9121 gccaccgcca gctcgtcatc aggacgggca aagtgatcca taccgataaa ctgatagccc
     9181 gattgcgtca ggaaggcgat ggtttcctgc aggatatcga gtttttgctg cggactcggc
     9241 aggtcagcat ctttgatttt gcgctgagca gcaaaaatgg tcggcagatg cgcgtagtta
     9301 aaaacgctca gacgatcggg gttcagctcc gccacacgtt tcagggtaaa ggcgaaactc
     9361 tccggcgtct gtttcggcag gccgtaaatc aggtcgatgt tggtggaggt aaaaccaatc
     9421 tcacgcgcat ggttaagcag tgcaaagatg aactcttcat cctgctcgcg gttaaccaga
     9481 cgttgcactt ctttgttgaa gtcctgcacg cccatgctca ggcgattaaa gccttcggcg
     9541 cgtaaatgat cgagtacatc cagttcgatt tcccgcggat cgacttcgat cgaaatctcc
     9601 gcatcggtat tgaaatggaa gttttcgcgc agcagcttca tcagacggct gatttgcgct
     9661 ttattcagat acgtcggcgt tccgccgccc cagtgcaatt ggctgacgtg acgcccggcg
     9721 aacagcggtg cacgatggac gatttcttgc tccagcgcgt ccagatactg atcggccttg
     9781 tgctgctggc gagtaacaat cttattgcaa ccgcagaagt aacaaagctt atggcagaac
     9841 gggatatgta cgtagagaga taatggacgc tcaggatagc gcgccacggc ttgtaaaaac
     9901 gcctgttcgc cgaagtcttc tgaaaactcc agcgcggtcg ggtacgaggt gtatcgtggc
     9961 ccggaatagt tatatttctg gatcagggcc agatcccagt cgatttgctg tacagacatg
    10021 ctcactcctt ccgatggcgt ctctggcggc tacggcgtac cggcggatgc ccgtttcggg
    10081 tcatcagccg gttgcgcagc cacttttgtc gccgcgacaa ccgctgtagt ttaacgaata
    10141 accacaccag ataacatata accggaaggg ttatcagcag aacgggaagg cccacggcgg
    10201 caatccgtta gttgccccgc agcagacgca tcatgtcttc ttgcttctcg tcttcttcct
    10261 cttcttcgtc atcgtcataa gagaggccga gtttctgcat caactcatca atacgatcca
    10321 gtttagcatc cacccaggat tgctcttcgg cgctcagggt ttcgcctgct tccagacgtt
    10381 ccagcagcgc atccagacgc tcatccgttt ccagtaattc caactccgcc tgcggtgaaa
    10441 gcataggttt ctcactcttc ggtttgtgct gtttggtgac tttttcagtc actcccaatg
    10501 gaataggggt tttactgcca atacgtggat cttttggtgc gttctggcct ttgctgcctg
    10561 acgtggtgtt tccgcccgct gcgcggctgc ccggcgcatg accacgacgt tttttctgac
    10621 gcttacggtc acgagcttcc tgatccagct cctcgcgtgt ttttcgacgc gcttttgcat
    10681 gacctttgct gcgtgagttt gaagatgatg gtttcataat ggttcgtctt ttagccgttt
    10741 tattcagtat agatttgcgg cggaatctaa cagaaagcaa gcaaagaaaa aaggcgacag
    10801 attactctgt cgcctttttt cctgactcat aacccttaac gggtcttaca atccttgcag
    10861 gccgccaaca caccctgttt ttccattagc cggaacaatc cgtgttgatt ccatttccgt
    10921 ttaattacgt ctccggacgt ttgtcttcct gacaatcctc tgtcttcgcc tcctggcgct
    10981 cctgaccctc atcctgagtc attgttcctg ttagcgtcct cgctctatgg gtgctacttt
    11041 acgcctttgc tttaaacaaa caatcaaccg ccatcagtat ttgcacattt tcagagtttt
    11101 atataaaagc aattcactga tttatatagc ttatttgaca tgcatcgcag aattttctct
    11161 gctatttcgc ataaagacta tcggcaatgt ctcacaaagc gtatggcttt tacttacaac
    11221 gctcacgccg aaaaatccgc cttttattat ggttcaggta taatcgccac aaagctttga
    11281 ctgacggaga cgaccgcttt gactaatttg aattatcaac agacgcattt tgtgatgagt
    11341 gcgcctgata ttcgccacct accttccgat accggaattg aagtggcttt tgcaggccgt
    11401 tccaacgcag gtaaatccag cgcgctgaac acgctgacta accagaaaag cctggctcgt
    11461 acctcaaaaa ccccagggcg cacccagctt atcaacctgt ttgaagtggc tgacggcaag
    11521 cgtctggttg acttgcctgg gtacggttat gcggaagtcc cggaagagat gaagcgcaaa
    11581 tggcagcgtg cgctcggcga atacctcgaa aaacgtcaga gcctgcaagg tctggtggtg
    11641 ctaatggata ttcgccatcc gctgaaagat ttggatcagc agatgattga gtgggcggta
    11701 gacagcaata tcgccgttct ggtgctgctg accaaagcgg acaaactggc aagcggcgca
    11761 cgtaaagcgc tattgaatat ggtgcgtgaa gctgtactgg cgtttaacgg tgatgtgcag
    11821 gttgaaacgt tttcttcgtt gaagaaacaa ggcgtagaca agctgcggca gaaactggat
    11881 acctggttta gcgagatgca gcctgtagaa gaaacgcagg acggcgaata attttcttgc
    11941 cttaatgctt gtgccggatg tggcgtatcc ggcccgtaaa ttcaatagat tacaattcta
    12001 ccgttggcct gataaacata gcgcatcagg cattacggat cttttctttc gcccaataaa
    12061 aaacgcccca gtcattactg actggggcgg ctaaaatatt cagccaaatc cgattacgtg
    12121 aagtaaaagg tctgaaagat agaacatctt acctctgtac cctacgcgac taactttact
    12181 cttttttgtt cagttcagaa agcacttttt gtaatttttt ttcagttgtt gcataggaat
    12241 ttctaatagc catcacaaaa cgtgacagct tatgttgctt acttacgaaa aaagggcatg
    12301 ttcaggcgaa tcttagtgcg cctgatccca gttttcgcca ctccccactt ccaccagcaa
    12361 cggcacatcc agacgggtac agttttccat cagttgatga atctgcttcg cgacggcatc
    12421 aacatcatct ttatgaactt caaataccag ttcatcgtgt acctgcatga tcatacgtac
    12481 acgcggttgc tcagcctgta accacgcatc aacggcaatc atcgcccgtt tgataatgtc
    12541 ggcggcggtt ccctgcattg gcgcgttaat ggctgcacgt tcagccgctg cacgacgagc
    12601 accattgctg gatttgatat ccggcagata cagacggcgt ccgtccagcg tttcaacgta
    12661 gcccagctct ttcgcctgag cacgggtgcg ttccatatac tccagcacgc cagggtagcg
    12721 ttcgaagtaa aggtccatgt acttctgcgc ttctttacgt ggaatgttca attgccgcgc
    12781 cagaccgaaa gcactcatgc cataaatcag accaaagttg atcgctttcg cgctacggcg
    12841 ttgctcgctg gtgacggttt ccagtggcaa accaaacact tctgccgccg ttgcccggtg
    12901 gatatctttt ccttccgcga atgcggtcag caagccttta tcacgcgaga gatgcgccat
    12961 aatgcgcagt tcaatctgcg agtagtccgc tgagacaatc acataatcct ctggcgcaat
    13021 aaacgcctgg cggatacgac gaccttcttc gttacgcacc ggaatgtttt gcaggttagg
    13081 atcggttgac gataaacgtc ccgttgcagt tactgcctgg tgataagagg tatgcacacg
    13141 cccggttttc gggttgatca tcagcggcag cttgtcggtg taagtcgact tcagcttcgc
    13201 cagaccacga tactccagaa tcacttttgg caacggatag tccagcgcca gttcttccag
    13261 tacctcttcc gacgttgacg gcgcgccacc cggcgttttc ttcagcggtt taataccctg
    13321 tttttcaaac agaatggttt gcaactgctt ggtggaagaa aggttaaatt cttcacctgc
    13381 aatttcatgc gctttctttt ccagctcagc cagacgcaag gtgagctctt cagaatgatt
    13441 gtgcagcact ttcggatcga tcttcacacc gttacgttca atgcgtgaaa gcaccggcac
    13501 cagcggcatt tcgatattct cgaagacgtt caacggccct ttgtgttttt gcagatccgg
    13561 ccacattttc agatgcaact gcaaggtgac atctgcatct tcggcggcgt aacgtccggc
    13621 ctcttcgagg gcaatctggt taaaggtcag ttgatttttg cctttacccg caatctcttc
    13681 aaaagtgatg gttttgtgct tcaaccaacg ttccgcgagg ctgtccatat cgtgacgccc
    13741 ggcaacgcta ttgagaatgt aggactccag catggtatca aacgcaatcc cacgcagttc
    13801 aatgccgtaa ttcgccagaa taccgcgatc gtatttcagg ttttgcccga ccttcagcgc
    13861 cttttcatct tccagcagcg gttttagcaa ctcgagtgca cgctcgtgag agatttgatc
    13921 gggcgcatca agataatcat gagcaaccgg aatatatgcc gctacgcctg gctcgatagc
    13981 aaaagaaagc ccgaccaggt tagcagagat gttatcaagg ctgtcggttt cggtatcaaa
    14041 tgcaaatacc ggcgcttttt ccagcttcgc aatccacgct ttcagtgttt cttcatcaag
    14101 gatagtgacg tagttgtcat aagaaatcac cgttgccgtc acttctggcg cttcgtctgc
    14161 aacactggtt tcctgcggct tcgcggctgg ttttgcccct ttggcctgta accatttgcc
    14221 cgcttcgaca tcagcagtcc agcgtttgaa ctcatacttt ttgaacagcc ccaacaactc
    14281 ttccgctgcc ggttgctgca cttccagttg ttcacaggtc agctccagtt caacatcggt
    14341 tttaatcgtc gccaactggt atgagagata agcaacttct ttgttttgct cgagcttcgc
    14401 tgccattgtt ttcgcgccac ggaagctcaa cccagcaatt ttttctggct cggcatacag
    14461 cgtatccagc ccgccaagac cttgcagcaa tgcctgcgcg gttttttcac cgacgcccgg
    14521 tacgccagga atgttatcag aggagtcacc catcagcgcc aggaagtcga tgatcaattc
    14581 tggcggcacg ccgtacttat tcaccacctc ttccggtccg aggatggtat tcgtcatggt
    14641 attgataagc gtaatatttg gcgtcaccag ctgcgccata tctttatcgc cagtgctaat
    14701 cagcaccgga cgcccggctt tttcggcttc gcgcgccaga gtaccgataa cgtcgtccgc
    14761 ttctacgcca gaaaccgcca gcagcggcag tcccatcgct ttaaccatcg cgtgcagagg
    14821 ttcgatttgt gctcgcagat cgtccggcat tggcgggcga tgtgatttgt aatgttcaaa
    14881 cagttcatca cgaaaggttt ttcccttggc gtcaaagacc accgctgcat gcgtcggttt
    14941 atattgcatg atcagactgc gcagcatgtt gaggacacca tacatcgcac cggtcggctc
    15001 gcctgcgctg ttagtcagcg ggggaaacgc gtgatatgcg cgataaagat aagatgaacc
    15061 atctacaagg ataagtggat tttgggggat ctgaaccata atgtccgtgc ctgtttatca
    15121 gattatgggt aaaggatgcc acagacagga tgaaaacatg agtttttcac cgcttttcgt
    15181 gaaaatcttt tgtagatctg ctggatcgct cgcaaatcgt catgtggata actttgtgca
    15241 taaattattt acatcgcttt taatttatta tcatgctttt tacagcatga tgtttgtctt
    15301 tatttttcat gcagttaaaa ataaatccat ccctaataat tgagataatg ggattttttc
    15361 taatgtggat agatatgaat tatttttctc cttaaggatc atccgttatt tgggtcattt
    15421 tctgctaaaa tctgcgcctt gcgcttcttt agtgcactgt ctatggctaa ccttttgaat
    15481 aaattcatta tgacgagaat actcgctgcg ataacccttt tgctgagtat cgtattgacc
    15541 attttggtca ctattttttg ttccgtcccg atcatcattg ccgggattgt aaaactattg
    15601 ctacctgtgc cagtcatttg gcgaaaggtt tcgcgttttt gtgattttat gatgtattgc
    15661 tggtgtgaag gtctggcggt attactgcat ctgaacccac acctgcaatg ggaagttcac
    15721 gggctggaag ggttaagtaa gaagaactgg tatctgctta tttgtaacca ccgtagttgg
    15781 gcagatattg tcgtactgtg cgtgctgttt cgtaagcaca ttccgatgaa taaatatttc
    15841 ctcaagcaac agctggcctg ggtgccattt cttggcctgg cgtgctgggc gctggatatg
    15901 ccgtttatga agcgttattc ccgcgcttat ttattacgtc atccggagcg acgtggtcaa
    15961 gatgttgaaa ccactcggcg ttcttgtgaa aagtttcgcc tgcatcccac caccattgtc
    16021 aatttcgttg aaggctcccg tttcacgcaa gaaaaacatc agcaaaccca ctccactttt
    16081 caaaacttgt tgccaccaaa ggcggcaggc attgcgatgg cgctaaatgt actgggtaaa
    16141 caattcgata aactgcttaa tgttacgctg tgttatccgg acaataaccg tcagccgttc
    16201 ttcgatatgt taagcggtaa actgacgcgg attgtcgtac atgtggattt acagcctatt
    16261 gccgatgagt tacatggcga ctacatcaat gacaaaagct ttaagcgcca ttttcagcaa
    16321 tggctgaact cattgtggca ggaaaaagac cggcttctga cgtctctcat atcatcacag
    16381 cgtcagaata agtaagtttt actcgcctct gaatgagcag aggcgagtga gtattttaat
    16441 gaacaaaacg tccggcacga gacataaatt cttcttcgct catctcctgt ccgttaaaaa
    16501 caactttacc cggtgtataa cgcagctgca gcgaggcggt attgttgtca atcgtgatta
    16561 actgaaacat ctgacctaat gtcatcatcc cgctgatttg tttatcagcc tgcttttgcg
    16621 ctttttccgc atccattcct tcagataaat taagctgttt tgcggtttct gtgacgacat
    16681 taagcggtaa cttcacatca aaattgagcg atttgatatc tttgtttgtg gatgatgaag
    16741 atttggctgg gtcggcaata ctgatatcca gattggcatt tagttcgccg agtgcgttct
    16801 tccatcttac cggttgttta atggtcggct cacttttttg taataacggt aaatactctt
    16861 tgaacaatgc agcattcact tcttgcagag caacttcatc gtttgctaac tcagggtgtg
    16921 cagcaagctg cttctgcatc gcaatgttat actggatgat aaattggcgc actgctgagg
    16981 gatcaataga ttcagcaatc aatgaccact caccactacc gagttgctga ttcccttttg
    17041 ttaacttatc aagttcgtag gtcagattga cattgatata atctttgtct ttaacgcgat
    17101 ccagcctggt ttttgcaacg aaagcatcga tttgggcaac gtcttccccg tgattgataa
    17161 tgttaatctg attaaatttc gactcgcttt caccaaccgg aaatttcttt tcttctaatc
    17221 gcgccagaga atcaatggta aaactttttg ccgttacttt gttcattggt gataattgca
    17281 gaacaatatc ttcgactttg ccttcaatat ccaggttaga caatgaacta ccatcaccat
    17341 taaaagtaaa ctggccctca ccaaaagaaa actttccata ctctgcaggg gcaacatcta
    17401 atgtcgtaga gaatgagcca ccaaacgcga atcgcaatgt cgcttccacg ggcgatttat
    17461 ttttcgcggc gataaatagc ggttgtgtca gttcattatt cactacgttc acttttgccg
    17521 ctgccagtgc tgggatgaca ttaccatgca ttaacattgt gatgggcaac ggaccgtgct
    17581 caacatccac atcaaataca actttctggc ctggcttgat attgagatcg ggtgcaccgt
    17641 tatcgaatgt catttgcatt tgaatacggg aattaaaaaa gcctttgtca aagtttttat
    17701 aacttaacgt catatcgtag gcatgttcac ctttcttttt cgcatcgtta aaatctttaa
    17761 taaacttttc gataccaggc tgaatttgcg tacctgtgta ccatgtgcca ccaccccaga
    17821 ttacggctaa cgcaacaata acacctgtag ctgacttacg tatcatataa atattccctg
    17881 ataaaacagc aaaactttga atatccactt atgctgaaat aatgtccagc ggcaggatgc
    17941 attatcagaa tgtatttata tttattaaat aataaaaaaa gcccgtgaat attcacgggc
    18001 tttatgtaat ttacattgaa ttattttttc tcggacagat atttcaccgt atcagcatac
    18061 tgctgaacaa aaacatccat attgctggta tccataccct gcggattcag ctgatattta
    18121 ccgttaacaa acatcgccgg aacaccacgc aattgcacat cagctgcggc tttttcctgc
    18181 tgagcgacca gagatttcac cacgaagctg ttccacgccg cgtcgtactc ttcaccttta
    18241 atacctgcat tgataaatac atcgcggata tcagatgctg aacgaatggt ctgggttttc
    18301 tgtacgcctt caaacagcgg aacagtcact ttgtcttcca cgcccagcgc catcgccaca
    18361 gcccatgcct gagtcagatc tttgcccagg tcaccaccca tgaagttgac gtggtattta
    18421 gtcatcttca cgccttccgg cagttttttc ttcacattat cagaaatatg cagaacttct
    18481 tcaaactgat agcagtgcgg gcagaagaaa gagaaaaact ccagcacttg cggcgcgcca
    18541 gctaccggtt tttccagggt agtgtactgt ttaccatctt catactgcgc cgccgatgca
    18601 ctaaacgcta aaactaaacc agccagcgcc agccaaatct ttttcatgat ctactctctc
    18661 cgattaatac ataggtgtta attgcaaagg ggattcttgt agaacttttg cctgttctat
    18721 aaaagtcgcc gtctgtcgta gccagtaatc ttccccggtt aaccacggga aatttttcgg
    18781 gaacgcggga tcagcccaat gccgcattag ccaggcaaga taataaacca aacgcatggc
    18841 gcgtaaaggt tcaatcagtc cgatttcagc ggtgtcgaac tcgctaaatt cttcataagc
    18901 ttcaataata gtttccagtt gcatccgctg ctgggcttta tcgccattga gcaacatcca
    18961 caaatcctga atagctggac cattacgtgc atcatccaga tcaacaaaca ttggaccatc
    19021 gcgccagaga atattcccgg cgtggcagtc tccatgtagc cgcagaacgg tgaaatcttc
    19081 ccgccagtgt gctgtaacgg cggcgatcag ctcatctgtc gctttcagaa atgccgcttt
    19141 caacccggaa ggtatcagtg tagcgtcctc aaacagcttg cgaggctcaa tgagatattc
    19201 attcagaccg atggtcgggc gatgaataaa aagctgtttg cgccccgtct ggtgcatacg
    19261 ccctaaataa cgcccaaccg cttccatctg atcgatatta tcagcttcga actggcgacc
    19321 accgacgctt ggaaaaacag cgaaataaaa tccctgatga ttcaataaag tctgaccgtt
    19381 aaaggccaca ggtgctgcga ccggaacttc atcatttacc agctgcaacg caaattgatg
    19441 ttcttcgagg atttgatcgg ctgtccaacg ttcagggcga taaaatttga cgacaaaacg
    19501 tcgacgctct tcgtcctgaa attgatagac acggttttca tagctgttaa gcggggtaag
    19561 accggaatcc acccggatcc cttgctcaaa cagagcgtcc atgatggtat ccgggtgtag
    19621 tgtctggaaa gtaaaagcgc tgttgttcat cccatcatcc ggaaaacatt acgaatgatg
    19681 aaggatatca tttcacgatg attttggtgg cgccgcttac aagcttttac tctttaatta
    19741 cgccgcgagc gcgtaacagc gccgttttga agtcttcttc ataatctttc tgcaaacccg
    19801 ggatcaccgc atctttcgcg gaatcacgca ttttcaggtg atagatcaga atgtcatccg
    19861 tcaaatccgc cagttcgccg tcaaaacctg actctttcgc cagtttctgc aaaaattgca
    19921 acaagttaaa gtctggctct ttttgccagg ctggctggag gagttcaata acttcattca
    19981 gacgtttaca tttcatagta atgctcctta ctcttgagac agacacgtta gcagggtcaa
    20041 tcccacaata aaagaggcga tatcggtgaa tctgatgacg acgataacag gcgttgtgct
    20101 ggcaggcggt aaagccagac gaatgggcgg cgtagataaa ggattgctgg acttaaatgg
    20161 caaaccgctg tggcaacatg tcgctgacgc gcttaagacg cagctctctc acgtcgtggt
    20221 taatgctaat cgtcatcagg aaatctatca ggcaggcggt ctgaaagtga ttgaagattc
    20281 actggcggat tacccaggcc ctctggcagg aatgctttca gtaatgcagc aggaagctgg
    20341 tgagtggttt ttgttttgtc cgtgcgatac gccttacatt ccccatgatt tagccgcccg
    20401 gcttaatcat cagcgcaaag atgcgcctgt cgtgtgggtc catgacggtg aacgcgatca
    20461 cccgactatt gctctggtaa accgcgctat tgagccttta ttactggaat atctgcaagc
    20521 aggagaacgc cgggtaatgg catttatgcg tctggctggc ggtcatgcgg ttgatttcag
    20581 cgatcataaa gatgcatttg ttaacgtgaa tacgccagag gagcttgccc gatggcagga
    20641 aaaacgatga taccgttact cgcctttgcc gcgtggagtg gtaccggaaa aactacgctt
    20701 ttgaaaaaac tgatcccggc attatgcgcc agagggatcc gtccggggct gattaagcat
    20761 acgcaccatg atatggatgt tgataagcca ggcaaagata gctatgagct gcgcaaggct
    20821 ggcgcggcac aaaccatcgt tgccagccag caacgatggg ccttgatgac ggaaacacca
    20881 gacgaagaag agctggatct acattttctc gcaagtcgaa tggatacctc aaagctggat
    20941 ttgattctgg tcgaagggtt taagcatgaa gagatcgcaa agattgtgct gtttcgcgat
    21001 gcagccggac atcgacctga agaattagtg atagacaggc atgttattgc tgtagccagt
    21061 gatgtgccgc ttaatcttga tgtcgcatta ctggatatta atgatgttga ggggctggct
    21121 gattttgtgg tggagtggat gcaaaagcag aacggataag caagagtagg ctggatcaga
    21181 cgcagccagt gtcgcatccg acctttttct gcaccag
//
LOCUS       DADMEQ010000062        21035 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.062, whole genome shotgun sequence.
ACCESSION   DADMEQ010000062 DADMEQ010000000
VERSION     DADMEQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.062"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(703..3273)
                     /locus_tag="JNZ05_002353"
                     /pseudo
     CDS             complement(703..3273)
                     /locus_tag="JNZ05_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189288.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            3391..4584
                     /locus_tag="JNZ05_002354"
     CDS             3391..4584
                     /locus_tag="JNZ05_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAY8565565.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLAQAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKTTQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4782..6035)
                     /locus_tag="JNZ05_002355"
     CDS             complement(4782..6035)
                     /locus_tag="JNZ05_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAY8565566.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDAAGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6362..7552
                     /gene="hmpA"
                     /locus_tag="JNZ05_002356"
     CDS             6362..7552
                     /gene="hmpA"
                     /locus_tag="JNZ05_002356"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HAY8565567.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(7597..7935)
                     /gene="glnB"
                     /locus_tag="JNZ05_002357"
     CDS             complement(7597..7935)
                     /gene="glnB"
                     /locus_tag="JNZ05_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HAY8565568.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7996..9330)
                     /gene="glrR"
                     /locus_tag="JNZ05_002358"
     CDS             complement(7996..9330)
                     /gene="glrR"
                     /locus_tag="JNZ05_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HAY8565569.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9320..10033)
                     /gene="qseG"
                     /locus_tag="JNZ05_002359"
     CDS             complement(9320..10033)
                     /gene="qseG"
                     /locus_tag="JNZ05_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HAY8565570.1"
                     /translation="MRHIFQRLLPRRPWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10198..11625)
                     /gene="qseE"
                     /locus_tag="JNZ05_002360"
     CDS             complement(10198..11625)
                     /gene="qseE"
                     /locus_tag="JNZ05_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HAY8565571.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRIIWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12183..16070)
                     /gene="purL"
                     /locus_tag="JNZ05_002361"
                     /gene_synonym="purI"
     CDS             complement(12183..16070)
                     /gene="purL"
                     /locus_tag="JNZ05_002361"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAY8565572.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16328..17884
                     /gene="mltF"
                     /locus_tag="JNZ05_002362"
     CDS             16328..17884
                     /gene="mltF"
                     /locus_tag="JNZ05_002362"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HAY8565573.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17881..18417)
                     /gene="tadA"
                     /locus_tag="JNZ05_002363"
     CDS             complement(17881..18417)
                     /gene="tadA"
                     /locus_tag="JNZ05_002363"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAY8565574.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(18442..19077)
                     /gene="yfhb"
                     /locus_tag="JNZ05_002364"
     CDS             complement(18442..19077)
                     /gene="yfhb"
                     /locus_tag="JNZ05_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HAY8565575.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19286..20134
                     /locus_tag="JNZ05_002365"
     CDS             19286..20134
                     /locus_tag="JNZ05_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY8565576.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(20473..21027)
                     /gene="pinE"
                     /locus_tag="JNZ05_002366"
                     /pseudo
     CDS             complement(20473..21027)
                     /gene="pinE"
                     /locus_tag="JNZ05_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
BASE COUNT         5051 a         5683 c         5355 g         4946 t
ORIGIN      
        1 gtagttaaaa ataaaaggca tcacagtggt gaataatgcc gctttcggcc aggccggatt
       61 aatcgccgtc aattcagcgc gagcagactc atattgcgca acctccccga gtcccgcgga
      121 cgccagcatc gccaccatca ggcatttttc tttatgctgc tgttgtaaat cgccatacag
      181 cgataacagg tcaggttgtg aaacagcaaa gaaatccgcc tcaatactgg ttttcgccat
      241 ctcttgcgcc cactgtttca tttcactaaa cagttgctgt gcggtttgct gttcacccag
      301 cagtcgcagc gccattcctt gccagaagag ataatcaacc ggctgatcgt tgtaataact
      361 gtggatgtta atggtgcgat cgccggtcgc cgccagacgt aaacaacgca tcgcttcagt
      421 ttcatcgccc tgcgcgttgg cgcatatcgc ctgccagaac cagatgtcgt tatcagtttg
      481 ccccggtaaa cggccttcgc ttaaattctc cggataatgc agcgcggcat gaagcagttc
      541 gctggcctgc tgcggctctc tggcatcaag atgctgccag gcgcgtaata actggttgag
      601 gataaactga ctggtgacct tcccttcccc gccttcccac gggtggaatt tacgcgtggc
      661 gagaatgtcc gccgctttgt ctgcctgacc cgtgagatgc cacaaattga gcagttctgc
      721 ggtcatgtcg tcgcgtttca gcgcaatttc cagattattt tccagacgcg ccagtcgttt
      781 ctccggtgtg gctccactta acttatccag caaatcccgt tcgaaaagca gacgtgcatc
      841 ctgcggcgca agctgataag cattatcaag ataacgcgcg gccagctcat aatcgtgttt
      901 cttattccac gcatggatcg ctaacccgcg ccagccgtcg gcaaactccg gcgacatctc
      961 tacgcaacgt tgccataagg caatggcttt gttgtagcta cgtttgttgt agtagaagca
     1021 ggccagtaaa tggcgagcaa accagcactc ttcaatactc tccagcgccg ccacttcttc
     1081 cagcgtattc gggaaacgga caaactgcgg gaagacatca atggcttttg cgaccagttc
     1141 gccacgttcg gctttcggca gcaagctggc ttgcaggtaa agcggcagcg tgcgctggca
     1201 gtccagtgcg ttcagcatct ctgccgccag ggtgggcatt ccccagttaa tcagttgccc
     1261 ggcggtcatc agagcgttaa cgtcgcgtcc ctgacacaga ccgcgccact gcgcgagggc
     1321 tgattcgcta cgaccatcga accagttcag ccaccacaga gtggcgttca gcggataatc
     1381 acgcagcagt ttctcgcgct gcaaacgcgc gttgtcctga cgaccactta acaccagcag
     1441 cagattatgc aagcaaagca cttcctgatt ggttgggcag gcacgaagac tttgttggca
     1501 aaaatccaga cccgcgtcga agttatcatt acgcgccgcc agtcgtgcca gaccataata
     1561 gccgcctgct ttactattgc cgctccagac cgcacgccag aaatcctctt cggcttgttg
     1621 atattgtccc tgacgttcgt aagcactggc gcgaatcaaa cttgcttgtc cacactgcgg
     1681 gtttttgttc agcgcatgtg cgcgtttcag cgcctgactg gcatacgcca ccgcttgcgg
     1741 gaaatctgcg cggttatatt ccagcatcgc cagcgccagg ttacagcgat aatccagcgg
     1801 gtccagcgcc acgccgcgca ggtagtaatc gaacggtgaa cggctggcgt gatgatattg
     1861 ctccagatgc tgaccgataa accaggcttc atctgtactg gtaatgtctt gtgctgccag
     1921 tggcgctttg gcgacgtccg gcaacggcaa ctcctgcggc tgatgttcct gataactcag
     1981 tacaatattg ccgtcggcat cggagagttc aatggtcagt ctgtcaggat tgataccgtg
     2041 caacacgccc tggatggcgg tcgctggtgt cagggccacg gcatcatcga gcaacgcgtt
     2101 gcatttgccg atttcgcgga tcgccagacg gtatccgttc aacggagaga tggcatacag
     2161 cccccactca atcccccgct cactacgctg gagttttatc accgcatcgc gggaggcatt
     2221 ttgcaccatg cacagagaat gataaggcag gaaatactgc tcgaaacgct tctcttcgta
     2281 agcatcaagc caggtaaaat caggctggtt atcggcaaaa ataccggtca tcagttcgat
     2341 atacgggccg ttattgtcgg tcagactctt atcccacgcc tggccaaatt cactgtgtcc
     2401 ccaactccac tgttttttac ctggcgcaat atggtggttg gcaacgtgca gcaaaccgcc
     2461 atcttcatcg tgacaccacg cgccgacaaa atcgtactgc gatttttcgg ccatatatga
     2521 ggttggaaca ggcacatttt tatagcgaga aatgtccact ccggcggagt agtccacttt
     2581 gtagtaagtg ccggtggcga tggggaaagc ggagacggcc cgtttgccgt gatcaaacac
     2641 cgcagtcaca tccggtggga aaacgctctg atgcccttca ccccctttca ctgccgggtt
     2701 ggcccaccac aagaaatgac gcggcgtggc gttgccgtta tagacgcggc tggcgatttc
     2761 cagtgccgcc cggtcagggc gcagggtgaa acctgtcatc acctgtaaac catgcatcgg
     2821 ctccgtttcg ccgacccaca ccgtctgtgc accgtcttca tgggcttcga gggtgaaatc
     2881 aacgggcata aaggtggtcg ggcgatggtg ttgcggccag ttaaactcaa tcccgccgga
     2941 gatccacggt cccagcagcc ccaccagcgc aggtttaatg acttcattgt gatagacaaa
     3001 atcgcgctgt ttcactttat cccatgcgcg atgcacccga ccgcccagtt ccggcaggat
     3061 catcactttg atgtagtcgt tttccagcca caccgcctgc taggatttca gggttttctg
     3121 ctcgctcagc gtatcggtca cgccgtaggg ataaaccgcg ccggacgatc cctgataaac
     3181 gcgattttcc aggaacatgg gatgtatatc ctgcggcccg gtttcatagg tcgggatctc
     3241 aacgcgctct tgccacactt ttactggagt catggtgccc tcaatattaa caagacatac
     3301 tgaattaaaa gatttgtggc agtgtattga acaatctggc aatgttttcg cggaataatc
     3361 acgcaattaa ctaaacaagg tttagtgaag atgagagcct gcattaacaa tcaacagatt
     3421 cgccaccata acaaatgcgt gattctggaa ctgctgtacc ggcaaaagcg cgccaataaa
     3481 tcaacgctgg cccggctggc gcaaatttcg attccggcag tcagtaatat tttgcaggaa
     3541 ctggaaagcg aaaaacgggt ggtgaatatt gacgatgaaa gccagacgcg cgggcatagt
     3601 agcggtacat ggctgattgc gccggaaggt gactggacgc tgtgcctgaa cgtgacgccc
     3661 accagtattg agtgtcaggt tgctaatgct tgtttaagtc cgaaaggcga atttgagtat
     3721 ttacagattg atgtaccgac gccgcaggcg ctgctgtccg aaatcgaaaa atgctggcat
     3781 cgccaccgta aattgtggcc ggaccgcacc atcaatctgg cgctggcaat ccacgggcag
     3841 gttgatccgg tgaccggcgt atcgcaaacc atgccgcaag cgccgtggac aacgccagtt
     3901 gaggtgaagt atctgctgga agagaagctg ggcattcggg tgctggtcga taatgactgc
     3961 gtgatgctgg cgcaggcgga gaaatggcaa aataattcgc aggaacggga tttctgcgtg
     4021 atcaacgttg attacggcat tggctcgtcg ttcgtgatta acgagcaaat ttatcgcggc
     4081 agtttgtatg gtagcggaca gattggtcac accatcgtta atccggatgg cgtcgtctgc
     4141 gactgtggac gttacggctg cctggaaacc gtcgcctcgt taagtgcatt aaaaaaacag
     4201 gcgcgggtat ggctaaaatc acaaccggtt aatactcagc ttgatcctga aaaactgaca
     4261 accgcgcagt taatcgctgc ctggcagagt ggagaaccgt ggatcaccag ctgggttgat
     4321 cgctctgcca atgccattgg tttgagtctg tataacttcc tcaacatcct caatattaat
     4381 cagatttggt tgtacggtcg cagttgtgcc tttggtgaga actggcttaa tactattatt
     4441 cgccagacag gatttaaccc gttcgaccgc gacgaaggac cgagcgtgaa aacgacgcaa
     4501 attggcttcg ggcaattaag ccgcgcacaa caggtgctgg gaattggcta tttgtatgtt
     4561 gaggcgcagt tacgacagat ttgatggcgc gataacgtag aaaggcttcc cgaaggaagc
     4621 cttgatgatc ataaacgaaa aattgcctga tgcgctacgc ttatcaggcc tacacggaga
     4681 ttgcaatata ttgaatttac aagattttgt aggcctgaga aggtgttcat gccgcatcca
     4741 acatgaacaa cgagcacatt gtcagcaaat caccgtttcg cttatgcgta aaccgggtaa
     4801 cgtgcgcaga tgtcgagaac tttacctttg atgcgctcga taacggcttc atcattgatg
     4861 ctgtccagca cgtcacacat ccagccagcc agttctttcg cttcagcttc tttaaagccg
     4921 cgacgggtaa tcgccggagt acctacacga ataccggagg tcacaaacgg gctcttcgga
     4981 tcgttcggta cgctgttttt gttgacggtg atgttagcac ggcccagagc ggcgtctgct
     5041 tctttaccgg tcaggttttt atcaaccaga tcaaccagga acaggtggtt atcagtgccg
     5101 ccggaaacca ctttgtagcc gcgctcgagg aacacttcta ccatcgcttt agcgtttttc
     5161 gcgacctgct gctggtaagt tttgaactca ggctccatcg cttctttcag agcaaccgct
     5221 ttaccggcga ttacgtgcat caacggaccg ccctgaccac cagggaaaac ggcagagttc
     5281 agttttttgt acagctcttc gctaccacct ttcgccagga tcaggccgcc gcgcggaccc
     5341 gccagggttt tgtgagtggt ggtagtaaca acgtgagcat gaggaaccgg gttcgggtag
     5401 acgccagcag caaccaggcc cgcaacgtgc gccatatcaa cgaacagata agcaccgatg
     5461 ctgtcagcga tttcacgcat tttcgcccag tcaaccacgc cggaatatgc agagaaacca
     5521 ccgataatca ttttcggctt gtgttctttg gcttgttttt ccagatcggc gtaatcgata
     5581 tgaccggcag catcgatacc gtaaggaacg atgttgtaca gtttaccgga gaagttaacc
     5641 ggagaaccgt gagtcaggtg accgccatgc gccaggttca tacccagaac ggtatcacct
     5701 ggttccagca gcgcggtgta gaccgcaaag ttagcctggg agccggagtg cggctggacg
     5761 ttagcgtagt cagcgccgaa cagttctttc gcacgatcga tcgccagttg ttcaacgata
     5821 tcaacatact cgcaaccgcc gtagtagcgt ttgcccggat aaccttcagc atatttgttg
     5881 gtcagctgag aaccctgcgc ctgcattacg cgcgggctgg tgtagttttc ggaggcgatc
     5941 agttcgatgt gctcttcctg acgtactttt tcctgctcca tagcctgcca cagttcggca
     6001 tcataatcgg caatgttcat ttcacgcttt aacatccgca tctcctgact cagctaacaa
     6061 taaaattttt ggcctttata ggcggtcctg ttggacaacg gcgaacagta taaccgaatc
     6121 attgtgcgat aacaggtctt gacaaaggaa tttacgcaaa cgattacctt aaggctacgc
     6181 aaggctttgg agaataaaga gcttgcaacc ggaaacggat ttcttttcag gtttgtgatg
     6241 caaatttttc acttcatcac attctttctg aaaaacacca aagaaccatt tacattgcag
     6301 ggctattttt tataagatgc atttgagata catcaattaa gatgcaaaaa aggaagacca
     6361 tatgcttgac gctcaaacca tcgctacagt aaaagccacc atccctttac tggtggaaac
     6421 ggggccaaag ttaaccgccc atttctacga ccgtatgttt actcataacc cagaactcaa
     6481 agaaattttt aacatgagta accagcgtaa tggcgatcaa cgtgaagccc tgtttaacgc
     6541 tattgccgcc tacgccagta atattgaaaa cctgcctgcg ctgctgccag cggtagaaaa
     6601 aatcgcgcag aagcacacca gcttccagat caaaccggaa cagtacaaca tcgtcggtga
     6661 acacctgttg gcaacgctgg acgaaatgtt cagcccgggg caggaagtgc tggacgcgtg
     6721 gggtaaagcc tatggtgtac tggctaatgt atttatcaat cgcgaggcgg aaatctataa
     6781 cgaaaacgcc agcaaagccg gtggttggga aggtactcgc gatttccgca ttgtggctaa
     6841 aacaccgcgc agcgcgctta tcaccagctt cgaactggag ccggtcgacg gtggcgcagt
     6901 ggcagaatac cgtccggggc aatatctcgg cgtctggctg aagccggaag gtttcccgca
     6961 tcaggaaatt cgtcagtact ctttgactcg caaaccggat ggcaaaggct atcgtatcgc
     7021 ggtgaaacgc gaagagggtg ggcaggtatc caactggttg cacaatcacg ccaatgttgg
     7081 cgatgtcgtg aaactggtcg ctccagcagg tgatttcttt atggctgtcg cagatgacac
     7141 accagtgacg ttaatctcta ccggtgttgg tcaaacgcca atgctggcaa tgctcgacac
     7201 gctggcaaaa gcaggccaca cagcacaagt gaactggttc catgcggcag aaaatggcga
     7261 agttcacgcc tttgctgatg aagttaagga actggggcag tcactgccgc gctttaccgc
     7321 gcacacctgg tatcgtcagc cgagcgaagc cgatcgcgct aaaggtcagt ttgatagcga
     7381 aggtctgatg gatttgagca aactggaagg tgcgttcagc gatccgacaa tgcagttcta
     7441 tctctgcggc ccggttggct tcatgcagtt taccgcgaaa cagttagtgg atctgggcgt
     7501 gaagcaggaa aacattcatt acgaatgctt tggcccgcat aaggtgctgt aatttgatgt
     7561 tgccggatgg aaacatccgg caacccttga cgcggtttaa attgccgcgt cgtcctcttc
     7621 accggtacgg atgcgaatga cccgagccac gtcaaagacg aagattttac cgtcaccgat
     7681 tttgccggtt tgcgccgtgc gaataatggt atcgacacag gtatcgacaa tgtcgtccgg
     7741 tacgacaatc tcaattttca ctttcggcag aaaatccacc atatactccg cgccgcggta
     7801 cagctcggta tggcctttct ggcgaccaaa gcctttcact tcggtcaccg tcatgccggt
     7861 aataccgact tcggccagtg cttcgcggac atcgtccagc ttgaagggtt ttataatcgc
     7921 atcaatcttt ttcatgctat tccttgaaaa ggttgcctgt cttttgatct gctaaacgta
     7981 acacataacg ccaattcatt ccttgaaatc gtttgcatcc agctcgtgtc gggaaagcag
     8041 tttataaaat tctgtccggt tgcgccccgc cattctcgcc gcgtgggtga cgttgccttt
     8101 ggtgatttgc agcagcttac gcaaatagtt gagttcaaaa tgattacgtg cctcaacaaa
     8161 ggttggcagc gccgtatttt caccctccag cgcctgctcc accagcgcat cactaatcac
     8221 cggagatgag gtcagcgcca cgcactgttc aatcacgttg accaactggc gcacattacc
     8281 cggccagctc gcggtcatca ggcgtttcat cgcatcggta gagaacgcgc ggacaaacgg
     8341 tttatgtcgc tctgccgcct ggcgcaacag gtgatttgcc agtagcggaa tgtcttctgt
     8401 gcgctccgcc agtgccggaa ttttcaggct gacaacgttg aggcggtaat agagatcttc
     8461 gcggaattcc ccgcgcgcca tcgcttttga cagatcacgg tgagtggcag aaataatccg
     8521 cacattgata tcaatatcgc ggttactgcc cagcgggcgc actttacgct cctgcaacac
     8581 gcgcagcagt ttgacctgta acggcgcagg catatcgcca atttcatcga gaaatagcgt
     8641 tccgccttcc gccgcctgga ataaaccttc gcgattgctg acagcgccag taaacgcgcc
     8701 acgcgcatga ccaaacagct ccgactccag caattgctcg ggcaatgcgc cacagttaat
     8761 agcaataaat ggtttgctgt tgcgcgggct ggcgttgtgg atagcctggg cgaaaatctc
     8821 tttcccggtg ccgctctgac cgttaatcaa aacgctgacg tctgattgcg ccaccagccg
     8881 cgcctgttcc agcaaacgca gcatcagcgg gctgcgggtg acaattgcct cgcgccagcg
     8941 ttcatcggtg gctggcgcgg attgctccag cgcatcgtca attgcctgat atagcgcatc
     9001 tttatcgaca ggcttggtga ggaaactaaa aacgccctgc tgtgttgcag caacggcatc
     9061 ggggatagaa ccatgcgcag taagaataat tactggcatt cccggctgca ctttctggat
     9121 ttcagcaaat agctgcatac cgtccatttc atccatccgc aggtcgctga taactaaatc
     9181 tactttttcg cgattcagta cccgtaatcc ttcagcgcca ctttccgccg tgaccacact
     9241 gtagccttcg ctggtcaggc gcaggccaag cagtttcagc aatcccggat cgtcatcgac
     9301 caataataaa tgcgcaggtt tatggctcat caggagtgac ctcatgggtg gatggcgcgg
     9361 gcttttcact ctcgtgcggc gtatccggcg agaaatttcc agccggtttg cgggtcgaga
     9421 gctggcgttc aatatcggtc aggttttcca gcttgcgggt ggtgagttcc agttgctgct
     9481 gtagaacgtg atgttgctgg cgcaatgtat ccagctcgct gtcgctggac tgctggagtt
     9541 tactgtaacg ttggcgctct tccgccagtt gcagttgcag cgcctgaccg tcgcgccaga
     9601 gttgatacag tgggcgaacc tgtgccggga tctcggtact taacgcttca atacgcgcga
     9661 ccagctggcg gcgctcatac ggcgtaattt tggcgtcggc gagcaaaatc ccttgtttaa
     9721 aggtattttg ccagctgccg tcgtcatatt ggcgagcttg ctgacgcgac tgcgcaggca
     9781 ttaaacgatc agcacaatcc atcgcccgca gccagtaaag cggattggtt tcggttgatt
     9841 taccttgcag cgcccagatg tcactacatt cagtagaaag atagtcagcc agttgataaa
     9901 ccggaatttt ttcttctgct ggcgtatcaa tggctggctt attgtgattc tgcacgcaac
     9961 ccagcaatgc cagacatggc agccctgcca gccacggccg tcggggcaat aatcgttgaa
    10021 aaatgtgtcg catattcacc agacttaaag cctatcccag tgggcgtaat tgttgcagac
    10081 agtctggaca tggacagcgc ggagaaaccg gggcgtacat atcgtacgtg aagatttcga
    10141 gcactacccg gggccgaact gacaaataaa atagcctgat aggataggct gttagcatta
    10201 tttcgtgttt ttcgacgacg gtaattcaat gcggaaacaa acgtcttgcc cgctctcgtc
    10261 gaccagatac agttcccctt gcatacggcg aatacaatcc ctggcaatgc ttaatcccag
    10321 accgctgccc ttcaccgccc cttttcgctg gtggcttccc tgaaaaaagg gttcgaagat
    10381 catggcgcgt tcctcttgcg gaatgggcgt gcctgtattg atgacatcaa tataaacccg
    10441 cgcaccatgt aaactgctgc gaaggcaaat gttaccggat tcagccccgt agtgcaccgc
    10501 attggagtaa agattatcca gtacgctcat cagcagcatt ggctccgcca ggcaagctgt
    10561 tgctttgaga tcgacgtcgg tatgcatcat tttagcccgt gcgggcaggc tatgagcaga
    10621 aaccactgtc tccaccagcg gtgctaattc aacattctcc agttccaccg cactgtccgc
    10681 ctgtttacgg ttgtaatcaa gcagttgttc gatcagtttt tgcaaattgc agctgctgct
    10741 atcaagaatg ctcaccacct ctttttgctc tggcgtaagc ggcccgacaa cctgatcagc
    10801 cagtaattca gtgccctcgc gcatactcgc cagtggcgtt tttaattcat gagataaatg
    10861 tcttaaaaat tgatggcgtt gggattccag ccatgacagg cgctcactta accaaataat
    10921 acgttgccca accgagcgta actcgctcgg tccacagaac gaaacgctat tgcccagaga
    10981 acgcccttcc cccagacggt tgatcatgcg ctcgatattt ttcaccggcc cgataatcat
    11041 ccgcgtgaaa agtagcacca tcaccagact caccagaaat agcaccagcg attgccaacc
    11101 aaaatattgc ccacgttcgg cgatttcacg ctgaagttgc tgcccacgag agaacaccac
    11161 tgtgcgcgtg gcctgcacca tttcggtatt ggcactggca aaggcttcaa gacgcgcggc
    11221 ggcggcagca tcgggaccac tgttgttaca ctgaagttgg gccagattgt tcaaatcctg
    11281 acgcaatgcc tggtaaagtt tatcgtctgg cagtacgcct gcgtgggcat cgagcatttc
    11341 gctgtaacgc ttgcgctggc tttgataaac cttcgcaagc gtagggtcgt ccagcacgca
    11401 atactgacgg tagctacgct ccatctccag cgccgcgttg gtcatcgctt cactacgccg
    11461 ggcatcaata agcgtagtgc ggttaaccag cgccgcctga tcgctaagcg cattcaggct
    11521 ttgccaggct tgccatgcca gcaccaacag gggtagtaga attagcaaaa atgccagcat
    11581 taccagttgt cgtaatgagc ggggaaaaac gggccagcgt ttcaaggtgt tactctcgtc
    11641 agacgcgaat agcctgatgc taaccgaggg gaagttcaga tacaacaaag ccgggaatta
    11701 cccggctttg ttatggaata aggcggtgcc taactcgacg tttcgcccga tggttgatat
    11761 agctacgctg atatcagaag ttggacggca ggcaccttgt tgtgcgtcat tcgaatttta
    11821 tgtagcacgt cccgaagggg ctgacataag tcggtgaatg agccactgct tactattatg
    11881 cagtaactgt gccaataaag aaaatagttt ggtaacgtac tgattatacg ttgctttgag
    11941 agatttatgg cttcttcgct atttgaatgt aaatcagcca ctgtgtcgct aaaaagagac
    12001 aacttaagat aaacttatta gattacaata aaatcaatca tttaagtgtc gccagaaagc
    12061 gacacggtaa accacccatt gtcgtgattt acagacacaa aaaagctccc ggagttggga
    12121 gcttatgata gtggttggtg cttaatgccg catccgggct gcaaaaccaa tgaactgatg
    12181 acttacccca actgcttacg cgcattgcgg aaaatgcgca tccatgggcc atcctcgccc
    12241 cagttttccg gatgccagga gttgctgaca gtacggaaaa cacgttccgg gtgcggcatc
    12301 ataatggtga ctcgaccact ttcagtcgta accgccgtaa taccgttcgg tgaaccgttc
    12361 gggttagccg ggtaggtttc agtgactttg ccgaagttat cgacatagcg cagtgccacc
    12421 agccctttgc tttccagtgc cgccagatgc gccgcatcac gcacttccac gcgcccttca
    12481 ccatgagaga cagcaatcgg catttgcgag cccaccatcc cctgcaacag cagagacggg
    12541 ctttgggtta cttcaaccag gctgaaacgc gcttcaaagc gatcggaggt gttacgcaca
    12601 aaacgtggcc acaactcgct ccccgggatc agttcacgca ggttagacat catctggcaa
    12661 ccgttacata cccccagggc cagcgtttgc ggacgatgga agaaggttgc aaactcatcg
    12721 cgtacacggt cattgaacag gatagacttc gcccaacctt caccggcacc cagcacatca
    12781 ccgtaggaga aacctccgca cgcgaccagg gcgtgaaaat cctccagtcc cgtacgtcca
    12841 gtcagcaggt cgctcatatg cacgtcgata gcatcaaagc ccccacgttg gaaagctgcc
    12901 gccatttcaa catgcgagtt cacgccctgc tcacgcagta cggcaacttt cggacgtgcg
    12961 ccagtggcaa tatacggtgc tgccacatct tcgttgatat cgaacgacag ttttacattc
    13021 aggcccggat cggcgtcgtt agatttcgcc tgatgctcct gatcggcaca ctccgggttg
    13081 tcacgcaggc gctgcatctg ccaggtagtt tctgcccacc agacacgcaa cgttgtgcgg
    13141 ctttcgctga atacagtctg cccgttggcg gtaatcacaa aacggtcacc ggaaaccgcc
    13201 tgcccaacat aatggacgca atccgcaagc ccatgctgtg ccagtacgga ctcgaccgct
    13261 tcacggtcag cggcacgaac ctgaatcacc gcacccagtt cttcgttaaa caacgccgcc
    13321 aggcgatcgt cacccagaga ggcgatatcc gcgtcaatgc cacaatgacc tgcaaaggcc
    13381 atttccgcca gcgttaccag caggccgcca tcagagcggt cgtgatacgc cagcagctta
    13441 cgctgtgcaa ccagcgcctg aatcgcgtca tagaagcctt tcagttggac aacatcgcgt
    13501 acatctgccg gtttgtcgcc aagctgacga taaacctgcg ccagcgccgt tgcgcccagc
    13561 gcgttattgc ctttgcccaa atcaatcagc agcagtgcgt tatcttcggt acaaagctgc
    13621 ggcgtgatgg tgtgacgtac atcttccacg cgggcaaatg cagaaatcac cagcgacagc
    13681 ggcgacgtca tttcgcgctc ttcgttacct tcctgccagc gggttttcat cgacatagag
    13741 tctttaccca ccgggatcgt caggcccagc gccggacaaa gttcttcacc cacggcttta
    13801 acggcttcat acaggcccgc atcttcacca gggtggcctg ccgccgccat ccagttggcg
    13861 gaaagtttaa tgcgtttgat atcgccaatc tgtgttgcgg caatattggt taacgcttca
    13921 ccgaccgcca gacgggcaga ggcggcgaaa tccagcagcg caaccggcgc acgctcgcca
    13981 atcgccatcg cttcaccgta gtagctgtcg aggctggcgg tagtgaccgc gcagttagcg
    14041 accggcacct gccacggccc caccatctga tcgcgcgcca ccatgccggt tacgctgcgg
    14101 tcaccgatgg tcaccaggaa agttttctcc gccacggtcg gcagatgcag tacgcgtttc
    14161 accgcgtctg caatggtgat cccttcacgg accagcgcat cgcccttcgc tttcagcgtt
    14221 tgtacatcgc gggtcatctt cggcgtttta ccaagcagga cgtccagcgg cagatcgatc
    14281 ggctgattat caaaatgacg atcgtgcaga gaaagatgca gttcttcggt cgcttcacca
    14341 atcaccgcgt agggtgcgcg ctcacgctta cacagttcgt caaacagcgg taattgatcg
    14401 gcagcaaccg ccagcacgta gcgttcctgg gattcgttac accagatttc cagcgggctc
    14461 atgcccggtt cgtcgcttag aatatcgcgc agttcaaatt taccgccgcg tccgccgtcg
    14521 ctcaccagct ccggcatcgc gttagaaaga ccgcccgcac cgacgtcgtg gataaacaga
    14581 attgggttag catcaccaag ctgccagcaa cggtcgatca cttcctggca acgacgctcc
    14641 atctccgggt tgtcgcgctg tacggaagca aagtcgaggt cggcatcaga ctgaccagac
    14701 gccatggaag acgccgcgcc gccgccaaga ccgatgttca ttgccgggcc gccgagaacg
    14761 accagcttcg caccgacgtt gatctcgcct ttttgtacgt gatcggcgcg aatgttgccg
    14821 atcccgcccg ccagcatgat cggtttgtga taaccgcgca gctcttcgcc attgtggctg
    14881 ttcacttttt cttcataagt acggaagtag ccgttcagtg ccggacgacc aaattcgttg
    14941 ttaaacgccg caccgcccag cgggccttcg gtcatgatgt ccagcgcggt aacaatgcgc
    15001 tcaggcttac cgaaatcttc ttcccacggc tgttcgaagc caggaattcg caggttggat
    15061 acggagaaac caaccaaacc agctttcggc ttcgcgccgc gcccggtggc accttcatcg
    15121 cggatttcac cgccggaacc ggtcgccgcg cccggccacg gagaaatcgc cgtcgggtgg
    15181 ttgtgagttt cgactttcat caggatgtgc gccggttcct gatggaaatt gtagcggccc
    15241 gtttcgtggt cagcaaagta gcggcccact tcagaacctt ccattacggc ggcgttatct
    15301 ttataagcag agagaacgtg atctggcgtg gtttcgaaag tatttttgat catcctgaac
    15361 agcgatttcg gctgttgttc accgtcgata atccagtcgg cgttaaaaat tttgtggcgg
    15421 cagtgctcag agttcgcctg agcgaacata tacagctcga tgtcgttcgg gttacgacca
    15481 agctttgtga aagcatcctg cagatagtca atttcatctt ccgccagagc caggccaaga
    15541 cgcaggttag cgtcgatcag cgcctgacgg ccctgcccca gcaaatcaac gcttgtgacc
    15601 ggagtcggtt gatggtgggc aaacaactgc tctgcatcat ctaaatcaaa aaagaccgtt
    15661 tccatcatgc ggtcgtgcag ttcagcggta acctgctgcc attgttcatt ggtcagcgta
    15721 ccggcttcta tatagtaagc aacgccgcgc tcaaggcggt ttacctgttg tagcccgcag
    15781 ttatgggcaa tatcggtcgc tttcgaagac cagggagaga tggtgccagg acgcggggtc
    15841 accagcagga gtttgccttg cggggcgtgg ctggcgagtg ccggaccata tttcagcagg
    15901 cgttcaagtt gtgcgtgctc atcatcgttt aacggcgcat tgaggtcagc aaaatggaca
    15961 tactcggcgt aaatattgtg aaccgggagc ctggcagcct gaaaacgtgc cagcagtttg
    16021 ttgattcgga atgccgacag tgcaggcgaa ccacgcagaa tttccatcat aagtctctcg
    16081 tcttctaagc tttcggtgta cccaaggggg ggaaacgggc gtcattataa agaatctgat
    16141 gcgctgacga aaccgtttgc gtggaaataa aatcaccatc gtgaattagc aacgcgtgcc
    16201 gccaatggct gtaataagtt gccatctggc gcaggtttac gcaaaatgcc gctcatttat
    16261 gagtaaacct ttcactatta ttacgttttt tcaagctggg acgcgcacga cacagagaat
    16321 taactaattg aaaaaattaa agattaatta tctgttcatc ggcattctgg cactgctgct
    16381 cgcggtcgct ctctggccat ccattccctg gtttggtaaa gccgacaacc gtatcgccgc
    16441 cattcaagcg cggggagagt tgcgtgtgag caccattcat actcccctga cttataacga
    16501 aatcaacggg aaaccttttg gcctggatta cgaactggcg aaacagtttg ccgattacct
    16561 cggcgtaaaa ctgaaagtga ccgtgcggca gaatatcagc cagctgtttg acgaccttga
    16621 taatggtaac gccgacctgc tggcggcagg actggtctat aacagtgagc gggtaaaaaa
    16681 ttatcagcct ggccctacct attattccgt gtcacaacaa ctggtttata aagtgggtca
    16741 gtatcgccca cgtacgctgg gcaacctgac ggcggagcaa ctcaccgttg caccgggtca
    16801 tgtggtggtt aacgatctcc agaccctgaa agaaacaaaa ttcccggaat taagctggaa
    16861 ggtagacgac aaaaaaggct ctgcggaatt aatggaagat gtcatcgaag gaaaactcga
    16921 ttacaccatt gctgattctg tcgccatcag cctgtttcag cgcgttcacc cggagctcgc
    16981 cgtagcgctc gatatcaccg atgaacaacc ggtgacttgg tttagcccgt tagatggcga
    17041 taataccctt tccgccgccc tgctcgactt cttcaacgaa atgaatgaag acggtacgct
    17101 ggcacgcatt gaagagaaat acctggggca tggcgatgat tttgattacg tcgatacgcg
    17161 cacattttta cgcgccgtcg atgcggtact gccgcagtta aagcccctgt ttgagaaata
    17221 cgccgaagaa attgactggc gtttgctggc cgctattgct tatcaggaat cgcactggga
    17281 tgcacaggcc acttcaccga cgggtgtgcg cggcatgatg atgttaacca aaaataccgc
    17341 gcaaagcctc ggcattacgg atcgtaccga tgccgaacag agcatcagcg gtggcgtgcg
    17401 ttatttgcag gatatgatga gtaaagtgcc ggaaagtgtg ccggagaacg aacggatctg
    17461 gtttgccctc gctgcgtaca atatgggcta tgcgcatatg ctggatgccc gcgccctgac
    17521 ggcaaaaacc aaagggaatc ctgacagttg ggctgacgta aaacagcgtc tgcctttact
    17581 tagccagaaa ccctattaca gcaagctgac ttacggctac gctcgtgggc atgaagccta
    17641 cgcttatgtc gaaaatattc gtaagtatca gattagcctg gtgggttatc tgcaagagaa
    17701 agagaagcag gctacagaag cggcgatgca actggcgcag gattatccgg cggtatcgcc
    17761 tacggagttg ggcaaagaga aatttccttt tctctcgttt ctttcccagt cgtcatcaaa
    17821 ctatttgacc cattctccct ctctgctgtt ttccaggaaa gggagtgaag agaaacaaaa
    17881 ttaatccgtc gaggattgcg cttttttctg cgctttaatt tcctggcggc gcatgcgaaa
    17941 gaagtcactg agcaacgccg cgcactcatc cgccagtatt ccttccgtaa tttccactcg
    18001 gtgattcata cccggatgat gcagcacatc cattaaagat cccgcagcgc cagttttcgc
    18061 gtcacgcgca ccaaagacca cgcgaccaat gcgactgtgg atcatcgctc cggcacacat
    18121 tacacatggt tcaagcgtga catacaacgt ggcgtcgatc agacgataat tttgcatcac
    18181 cagaccaccc tgccgcaggg ccatgatttc tgcatgtgcg gtgggatcat ggcgaccaat
    18241 cgggcggttc cagccttcgc cgattacccg attgttatgc actaataccg cgccgaccgg
    18301 cacttcccgc tcatcccagg cacgtttcgc cagcgtcagc gcgtgacgca tccagtattc
    18361 gtggctaaat tcgacttcag acaaaaagaa aactccggtt ataaaagcgc ggcgcattat
    18421 acacggacgc tatgctttac tctattccag ttgctggagt tcaccgcgcg gggttactcg
    18481 ccaacgatgc tgacagaaat aaagcagcgg attgtcctga ttactgtcgc tatagccact
    18541 gtacagccgc agcggcgtgc cgattttgcg ctccagttgt gcgacctttt catgtcccag
    18601 acaacgcatc gtcaataccc aaccaccata gccacgctga atttggctgg cgataagatt
    18661 aacccgcggc agccagggcg tatcgaaata aaccgcttca accagcggct gcggagagcc
    18721 ggtaatcaac cagatatcag catcggaact taacaggtag gtggttaatc gctcctgaac
    18781 cagcggaaag gcggtaacat tgtcgcgaaa ccagcgcacg aaatcggcct gcaacgtctg
    18841 taaacgtgct tcgctgtgac caaaagtgca cccccacaga agcagactca tcggccagcg
    18901 tgccgcacga ccttttatca ataacgcaat ggctataatc ggtaacaacg gcaggacaag
    18961 taacgcattc agcggttggc gacgcagtaa atagcgcaga aaactgccga acatatcctg
    19021 ctgatgcaat gttccatcta agtcaaaaaa caccacacga cgctcgtgag ttgccaaacc
    19081 atactcctct ggactgaaac atcctgatta attttcgcta ctacatagcc taacagatag
    19141 atcatcactt ttccggcaac aatcgaccgc atcatggcta actggaattt ttaattcatg
    19201 gcaattagcg gcaatggaat ataaaattca ctcgcgtgtg tctcatattg cctgttgtcg
    19261 ccactatttc atcgcgagga aagatatgaa cggcttactt cgtatccgtc agcgttacca
    19321 ggggcttgcc cagagtgata aaaaactggc ggattatctg ctgctacaac ctgatacggc
    19381 gcgccattta agctctcagc aactggccaa cgaagccgga gtcagtcagt ccagcgtcgt
    19441 gaagttcgcg caaaaactcg gctataaagg ttttccggcg cttaagttgg cgttgagtga
    19501 agcgctggca agccagccgg aatcgccctc cgtgcccatt cataaccaaa tccgcggtga
    19561 tgatccgtta cggctggtcg gcgaaaaact gattaaagaa aatactgccg ctatgtacgc
    19621 aacgctaaac gttaatagtg aagagaaact gcatgaatgc gtaacaatgt tgcgctctgc
    19681 gcggcggata attctgaccg gtattggcgc ttcgggtctg gtggcgcaaa actttgcctg
    19741 gaagctgatg aagattggct tcaatgctgc cgcactgcgc gatatgcatg cgctactcgc
    19801 aacagtacag gcgtcgtccc ctgacgatct gttattagcc atttcctaca ccggtgtacg
    19861 acgcgagtta aacctggcgg cagatgagat gctgcgagtg ggcggaaaag tgctggcgat
    19921 taccggcttt actccgaatg ccctgcaaca gcgtgcttct cattgcctgt ataccattgc
    19981 cgaagaacaa gcgacaaaca gtgcttcaat ctctgcttgt cacgctcagg gaatgttaac
    20041 ggatttgctg ttcattgcgc tgattcagca ggatctggaa ctggcaccag aacgtattcg
    20101 tcatagtgaa gcgctggtga aaaaactggt ctgagtaaag aatgcgcgta taatgcccgc
    20161 ccggtttgtg ttgttttgag agtttcctta tggcgctgtt aatcactaaa aaatgcatca
    20221 attgtgatat gtgtgaaatc aaataaaaac acatttattt tcatagtgat acagcaatgt
    20281 gttattgaat gtgttgttgg atttatggtg caaaaaaatt ttgcataagt tcagcaacac
    20341 aaagaacacc actttcttta acgtcctccg cgccctaaag gacggggctt tacggcgcaa
    20401 aaggcctgtg acatacaaca ttaacttttc agtcaaccgt agcacgtcct tgtatacgcc
    20461 gtgtctgcgg ttttatataa ccctggctgg aaacctctta tacaaagtcg attcgccaac
    20521 atcatagatg atcgccacct tctggcgaga aactcctgat gcaattagtc gcccggcctg
    20581 cgcccattat tctggtgaaa gtttgggacg gcgaccgcca attcgtccct gtgcgcgagc
    20641 agcttccagt cctgcttttg ttcgttcaac aatcagttca cgttccattt cagccagggc
    20701 acccatcaca tggaagaaaa agcgtcccat tggtgtgctg gtatcaattg aatcagtcag
    20761 actacgaaag ttgatgcctc gttcgcgcaa ctcctccacc agcacgacaa gatgccgcat
    20821 actgcgcccc agccgatcca gcttccagac aaccagtgtg tcgcctgccg ataatgtcct
    20881 gagcagtttt ttcagccccg gtctttcgga ctttgtacca cttatcttgt cttcaaaaat
    20941 cagctcgcat cctgcacagt tcaacgcatt acgttgtaga tcggtattct ggtcatttgt
    21001 tgatacgcgt acatagccaa taagcatggt aaatc
//
LOCUS       DADMEQ010000063        20685 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.063, whole genome shotgun sequence.
ACCESSION   DADMEQ010000063 DADMEQ010000000
VERSION     DADMEQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.063"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="JNZ05_002367"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="JNZ05_002367"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HAY8565577.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="JNZ05_002368"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="JNZ05_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HAY8565578.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="JNZ05_002369"
     CDS             complement(2649..3200)
                     /locus_tag="JNZ05_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HAY8565579.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="JNZ05_002370"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="JNZ05_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HAY8565580.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5215
                     /gene="cybC"
                     /locus_tag="JNZ05_002371"
     CDS             4829..5215
                     /gene="cybC"
                     /locus_tag="JNZ05_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HAY8565581.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="JNZ05_002372"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="JNZ05_002372"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HAY8565582.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="JNZ05_002373"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="JNZ05_002373"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAY8565583.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="JNZ05_002374"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="JNZ05_002374"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAY8565584.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHTESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVTWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKFIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="JNZ05_002375"
                     /pseudo
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="JNZ05_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="JNZ05_002376"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="JNZ05_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HAY8565585.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTPGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="JNZ05_002377"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="JNZ05_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HAY8565586.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="JNZ05_002378"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="JNZ05_002378"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAY8565587.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="JNZ05_002379"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="JNZ05_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HAY8565588.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="JNZ05_002380"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="JNZ05_002380"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HAY8565589.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="JNZ05_002381"
     CDS             complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="JNZ05_002381"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAY8565590.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKNKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="JNZ05_002382"
     CDS             complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="JNZ05_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HAY8565591.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18174..18569)
                     /locus_tag="JNZ05_002383"
     CDS             complement(18174..18569)
                     /locus_tag="JNZ05_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY8565592.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTEVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="JNZ05_002384"
     CDS             complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="JNZ05_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY8565593.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19485..20078
                     /locus_tag="JNZ05_002385"
     CDS             19485..20078
                     /locus_tag="JNZ05_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY8565594.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20223..20675
                     /locus_tag="JNZ05_002386"
     CDS             20223..20675
                     /locus_tag="JNZ05_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY8565595.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
BASE COUNT         4917 a         5209 c         5489 g         5070 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc ggatggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgcta gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggctcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaaa catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtagag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgccgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga
     6901 ctttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaag ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gacgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtagt tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gatgaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgtcac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agccagcgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgt atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aggcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tcgagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt ccggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg ggtggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gctatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccgggc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgaccttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acacaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctccgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg atcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtcagg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc caggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag
    17581 agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg ttcttcaaca
    17641 gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat
    17701 cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt
    17761 atctcctgac gcctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca
    17821 acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa
    17881 cacactgaac catacatcct cccggaaaat tgtccggcat tatactcata gccagaagcg
    17941 gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa
    18001 aatatatgca tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat
    18061 tgcagacatt tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc
    18121 cattcatccg acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc
    18181 tcagggatac gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc
    18241 acctccgtcc agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg
    18301 tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa
    18361 gtgcatcccg cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc
    18421 tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc
    18481 aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca
    18541 gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca
    18601 gaagatttat atttaccggg agccgctttt agggggacga cgttagtaaa caaaacccag
    18661 acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat
    18721 caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca
    18781 taccagcgac ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat
    18841 cgcgcattgg cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt
    18901 tgatggtgat ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg
    18961 cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg
    19021 agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat
    19081 gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca
    19141 gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct
    19201 tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc
    19261 cagtctcttg tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat
    19321 tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga
    19381 ggatgagaac tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac
    19441 atgcattaca aaactgtttt aactttctgt caacaggttt tgtaatagtc actaaaaaac
    19501 aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa
    19561 aagtgctttt tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc
    19621 ttggtattaa ccccccgagc ctctacgcgg cttttggcag taaggctggg ttatttagcc
    19681 gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg
    19741 atcgtccggt aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc
    19801 aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag
    19861 cgcgtgatat tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg
    19921 ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag
    19981 ggctttctgc gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc
    20041 tggcggggga agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc
    20101 gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga
    20161 attgatcatc ttttcgtata acgatagaaa tgaaacattg ttttaattaa ggagtggaaa
    20221 agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg
    20281 cgattgagca tatcaaagca cacgttacgg cagaaacgcc aaagggcaag cacgatatcg
    20341 aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc
    20401 gtgcggagta ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca
    20461 tgaccttcag cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca
    20521 tcgcattttt gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg
    20581 ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg ccagcccagg
    20641 ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact tttcg
//
LOCUS       DADMEQ010000064        20668 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.064, whole genome shotgun sequence.
ACCESSION   DADMEQ010000064 DADMEQ010000000
VERSION     DADMEQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.064"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JNZ05_002387"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JNZ05_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HAY8565596.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMLRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQSEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JNZ05_002388"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JNZ05_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HAY8565597.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JNZ05_002389"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JNZ05_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HAY8565598.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JNZ05_002390"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JNZ05_002390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HAY8565599.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGRVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="JNZ05_002391"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="JNZ05_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HAY8565600.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGTRVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="JNZ05_002392"
     CDS             complement(8217..9110)
                     /locus_tag="JNZ05_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HAY8565601.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="JNZ05_002393"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="JNZ05_002393"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAY8565602.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLVEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /locus_tag="JNZ05_002394"
     CDS             complement(10007..10189)
                     /locus_tag="JNZ05_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HAY8565603.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="JNZ05_002395"
     CDS             complement(10241..11473)
                     /locus_tag="JNZ05_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAY8565604.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="JNZ05_002396"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="JNZ05_002396"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HAY8565605.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVVMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="JNZ05_002397"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="JNZ05_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HAY8565606.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16497)
                     /locus_tag="JNZ05_002398"
                     /pseudo
     CDS             complement(14278..16497)
                     /locus_tag="JNZ05_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16704..16988)
                     /gene="ihfB"
                     /locus_tag="JNZ05_002399"
     CDS             complement(16704..16988)
                     /gene="ihfB"
                     /locus_tag="JNZ05_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HAY8565607.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17148..18821)
                     /gene="rpsA"
                     /locus_tag="JNZ05_002400"
     CDS             complement(17148..18821)
                     /gene="rpsA"
                     /locus_tag="JNZ05_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAY8565608.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18932..19615)
                     /gene="cmk"
                     /locus_tag="JNZ05_002401"
     CDS             complement(18932..19615)
                     /gene="cmk"
                     /locus_tag="JNZ05_002401"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAY8565609.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19788..20552)
                     /locus_tag="JNZ05_002402"
     CDS             complement(19788..20552)
                     /locus_tag="JNZ05_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY8565610.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4968 a         5713 c         5017 g         4970 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttcctg gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg actgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcaaacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc agcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt gcttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactctg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgctg gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtacca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc atattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaaacct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc caccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcctcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcaccac tacatgttca agctgagcaa
    11821 cgtcacaccg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccagaccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatacgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcaatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgac cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggctatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagg ccagtcctgt gtcatagaga atcgctttgc cgtttctggc
    15001 tatcaccatt gccagccctt gcccgacatc aagcatgtac agctgccact cgtcaggtcg
    15061 aggtttttgc cacagcggcc agcacatcag caagcctcca gccacacaca ttactggcaa
    15121 cgttcgccag gcgtttaatc accataccac cagtaagaac catggggaaa atgatagcca
    15181 ttgccaacat tcaacaatgt tgatccaccc ttccggcaaa ctctttaacc cctgttccag
    15241 gattaacggc ccgctaaaat gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa
    15301 tgtcaccaag ggaattgcaa atagatttgc aataaacgag gtcagactaa tgccatgaaa
    15361 tatgacgatt tgcatgggca taagcaggag tgtgattccc agttgcagat ggatgaggga
    15421 aacaactgca cgcaataccg gcggcagttg ccactcagga cagggaaacc actgatacca
    15481 aaaaatcagt gccgcgaccg cagcggcaga gagccataaa ctttgcgaga gaatggcaac
    15541 aggatccatc agcaaaattg ccgccagaca acatatccat acatcccagc cactccactg
    15601 tcgcccactt aacttaagca ttccccacgt agcaagcgcc accacggtac gcaatgcagg
    15661 aggttgcatt cccgtcaacc aggcataaaa agcagcacag cagattccgc caattaatgg
    15721 tatttgccag tggatccagc gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc
    15781 agccagcaac gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc
    15841 agtatcgcgc atgatatttt tgatttcttt ggggacggat aaccgttccc ccatacctaa
    15901 accaagaata accgcattcc acggataggg ttgcagcgtt gtttgtagtg acgccagata
    15961 ctgtgcacgc aggctacaat tcggttcaat gacacttgcc tgcagaaaac ggccggtgag
    16021 cggctgatgc tgggcaatgg cataacgctg gctatcaaag ccgccatcat taagttggcc
    16081 atgaactgca cgaactttga gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg
    16141 aagttattcg ccatacagca caaggcctgg cgcagggaat atacgtcgac cttgtagata
    16201 agtaatttga ccgtaatgag tggtcatgcc gtcagttgca gtgatctcaa caattgcatc
    16261 ctgcgtcgcg ccagttaagg tttctcctgc ccacaaaatt tgctttgctg ataatatgcc
    16321 ccaaacgaaa aacagcaacg tcagcgcgat ataacggacg gttttaacag ggataaatgc
    16381 cagtacgcag gcgaagagag tcagaaacgc aagggttaat gtcctaggca attggggcaa
    16441 aatcagcaac ggaaaaattc cgcaaattat gcatacgccg actgtcgtta ttttcatgca
    16501 cccctccctg tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt gggaattttg
    16561 cgttccccga ggcacattca gaagaattta acgaaagggt gaaaactgct caaaactctg
    16621 cgaggcagct tccagttcct gattagagaa ttgaaaaaag cacccgacag gtgcttttct
    16681 cgcgttcaag tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg cagttcttta
    16741 ccaggtttaa agtgaggaac gtattttcct tccagttcta ctttatcgcc agtcttcgga
    16801 ttacgtccgg tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc gcggatttca
    16861 atacgctcgc cctgcgcaag agtcgaggcc atatgctcca gcatctcttt tactgcatct
    16921 tcaaccgtct tggcgggaat gtgcgattgc tgggtggcaa gtctttctat caattctgac
    16981 ttggtcatga ttcctccggt tccttaaagg caaattaagc ggctgctgtt gctgcattga
    17041 ttaagggcgg ccgtagccgc ccttagtgct tgattacagg acgaaacctg caatctgtca
    17101 agtaaactca acaaacttcg gaataaaaat cccgaagagt cagagaatta ctcgccttta
    17161 gctgctttga aagcttcagc cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta
    17221 acagttgcga ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga caggctgatt
    17281 gcgcggtttt tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga
    17341 accagggtag cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag gtaaccttca
    17401 acgccgtcag ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt
    17461 acgatagcgc ctttcttgtt cagagcaacc cagttgttga acggatcttc tgcgagctgt
    17521 ttaacgccca gggagatacg ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt
    17581 tcgtcgcctt ttttgtattc acgaactgct tcttcgcctg caacgttcca ggagatgtca
    17641 gacaggtgaa ccaggccgtc gatgccgccg tccaggccga tgaagatacc gaagtcagtg
    17701 atagacttga ttttaccttc aacacggtcg cccttgttgt gggtttccgc gaactgctgc
    17761 cacgggttag ctttgcactg tttcagccct agggagatac gacgacgttc ttcgtcgata
    17821 tccagaacca taacttccac tacatcgcca acgttaacaa ctttggacgg gtggatgttt
    17881 ttgttggtcc aatccatttc ggaaacgtgt accagacctt caacgccttc ttcgatttca
    17941 acgaagcagc cgtagtcagt caggttggtc acgcgaccag tcagtttggt accttccgga
    18001 taacgtttag cgatagctac ccacggatct tcgcccagct gtttcaggcc cagggataca
    18061 cgggtacgtt cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc cacgttgacg
    18121 atttcgctcg gatgcttaac gcgtttccag gccatgtcag tgatgtgcag caggccgtca
    18181 acgccgccca gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat acctttaact
    18241 tccatgcctt cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg
    18301 ataacggcac gacgagaaac aacaacgttg ttgcgcttct gatccagctt gattacttta
    18361 aattcaagct ctttgccttc caggtgcaga gtgtcacgca ccggacgaac gtctaccaga
    18421 gaacctggca ggaacgcacg aataccgttc agctcaacag tgaagccgcc cttaactttg
    18481 ccgttgataa caccggtaac agtttcagca tcttcgtaag ctttttccag cgtgatccag
    18541 gcttcgtgac gtttagcttt ctcacgggac agcagagttt caccgaagcc gtcttctact
    18601 gcgtccagag caacgtcaac ttcgtcacct acctggattt ccagctcgcc ctgggcgttt
    18661 ttgaactgct cagccgggat ggcggactca gatttcagac cagcgtcaac cagtactacg
    18721 tctttgtcga tagcaacaac aacgccacga acgatagaac ccgggcgggt ttcgatttct
    18781 tttaaggact cttcaaagag ttgagcaaaa gattcagtca tgtttaatct tcaggtttat
    18841 atttaacgtc cacctggctc catgccggat ggggttgttt aacatacccg ctgtcattcc
    18901 attgcaacgg gggtactgca aattcggtcg cttatgcgag agccaatttc tggcgcgcgt
    18961 attgtagcgc tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc aacactaaag
    19021 catcggctgc cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt
    19081 tgatctcggc caaaaggcgc tcgaagttaa cactaaagcc cttctcctgc aactgtagca
    19141 tgcggcgatg cgcacgttct tccgaggagg cgtcaaggaa aattttcact ggtgcatcag
    19201 ggaataccac cgttcccatg tcgcggccat cggcaatcag acctggtaat tcgcgaaacg
    19261 cgcgttggcg acgcaataat gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg
    19321 cattcgccac ttcctgagta cgaatttcgc cgctgacatc ttccccttcg aggatcactt
    19381 ccagattgcc attggtcgac acaaaacgta catccagatg ggatgccagc ggtaccagcg
    19441 catcttccga cgcaacatca acatggtgat gtaatgccgc caatgccagt acgcgataaa
    19501 ttgcacccga gtccagcaga tgccattgca acgcttccgc catagcctta cacaaggtgc
    19561 ctttccctgc accgcttggg ccatcaatgg taataaccgg ggcaattgcc gtcatcttta
    19621 tctccttaaa acaggcgtac cgttaacata accgcgcgca ttatacgcgc cgacgccaca
    19681 aatcgttaac gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg atttattgcg
    19741 caattatagc gggctggcag aagaccagcc cgaaagatga caatgattta cttaccagag
    19801 gcgatacgat cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg
    19861 gactgggtgc gaccgccatc caggctagcc agtttttcaa agctgccaac cagcccctgg
    19921 gtgctgatgc cacgtttctt caacagatca aaagagaaat catctgcttc ggactcttta
    19981 tcacgggagt atttagcatt aatagcgcct tctgcgatat cacccaattg tgagcgggaa
    20041 agctgggaag ccacaccgct ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa
    20101 gaagctttca tttcagccag cgagtgaccc aacgcgacgt ggcccagttc atggcccaga
    20161 acaccttcaa tttcgttgtc attcatcatg tccatcaggc cactgtagac acgaacacag
    20221 ccgttcgcca tcgcccatgc gttgacgtcg ctggtcatat aaaccttata gttgaccggc
    20281 gtgccattaa tattgttacc caatgctttg gcgatttttg ccagacgttt accgtacttg
    20341 ctggatttac ttgcgacttg attgccgctg tccatttcgg cacagccctg attggcaatc
    20401 gcttttgcat cggcatcgct taatgttgct gctttatagg catttaaacc ggagctgata
    20461 gccatattgg tatcaatacc gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc
    20521 gcaatcgcca gcagtaattt agtattcttc atgtgaatca tccgttgatt aatcatatat
    20581 aaatattatg tatcaacaac ttacattgac aagcgacgct accattaatc aaaatgactc
    20641 acaaggtccg aaaaaaaacg ccgacaat
//
LOCUS       DADMEQ010000065        20549 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.065, whole genome shotgun sequence.
ACCESSION   DADMEQ010000065 DADMEQ010000000
VERSION     DADMEQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.065"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="JNZ05_002403"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="JNZ05_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAY8565611.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="JNZ05_002404"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="JNZ05_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HAY8565612.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="JNZ05_002405"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="JNZ05_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HAY8565613.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JNZ05_002406"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JNZ05_002406"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HAY8565614.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JNZ05_002407"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JNZ05_002407"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HAY8565615.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="JNZ05_002408"
     CDS             4449..4814
                     /locus_tag="JNZ05_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HAY8565616.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISD
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            4964..5482
                     /locus_tag="JNZ05_002409"
     CDS             4964..5482
                     /locus_tag="JNZ05_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4154 domain-containing protein"
                     /protein_id="HAY8565617.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="JNZ05_002410"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="JNZ05_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="JNZ05_002411"
     CDS             6713..7195
                     /locus_tag="JNZ05_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAY8565618.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="JNZ05_002412"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="JNZ05_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAY8565619.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="JNZ05_002413"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="JNZ05_002413"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAY8565620.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="JNZ05_002414"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="JNZ05_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAY8565621.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="JNZ05_002415"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="JNZ05_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAY8565622.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="JNZ05_002416"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="JNZ05_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAY8565623.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10864..11730
                     /locus_tag="JNZ05_002417"
     CDS             10864..11730
                     /locus_tag="JNZ05_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HAY8565624.1"
                     /translation="MQRLEQRSPDAILLLFLIAQTVDITMPVFALLALVAYSVSLALI
                     VPGLLQKNGGWRRMAIISAVIALVCHAIALEARILPDGDSGQNLSLLNVGSLVSLVIC
                     TVMTIVASRNRGWLLLPIVYAFALINLALATFMPNEYITHLEATPGMLVHIGLSLFSY
                     ATLIIAALYALQLAWIDYQLKNKKLAFNQEMPPLMSIERKMFHITQIGVVLLTLTLCT
                     GLFYMHNLFSMENIDKAVLSIVAWFVYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLA
                     YFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="JNZ05_002418"
     CDS             11751..13037
                     /locus_tag="JNZ05_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY8565625.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="JNZ05_002419"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="JNZ05_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAY8565626.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="JNZ05_002420"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="JNZ05_002420"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HAY8565627.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="JNZ05_002421"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="JNZ05_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAY8565628.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="JNZ05_002422"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="JNZ05_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HAY8565629.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="JNZ05_002423"
     CDS             complement(16985..17275)
                     /locus_tag="JNZ05_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HAY8565630.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17741)
                     /gene="ratA"
                     /locus_tag="JNZ05_002424"
     CDS             complement(17265..17741)
                     /gene="ratA"
                     /locus_tag="JNZ05_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HAY8565631.1"
                     /translation="MILFVGFLLMEIVMPQISRTALVPYSAEQMYQLVNDVQSYPQFL
                     PGCTGSRILESTPGQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGG
                     WKFTPLSQEACRIEFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="JNZ05_002425"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="JNZ05_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAY8565632.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="JNZ05_002426"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="JNZ05_002426"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="JNZ05_002427"
                     /pseudo
     CDS             19137..20378
                     /locus_tag="JNZ05_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         5305 a         5363 c         4824 g         5057 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcgct ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc agtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 gccatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcattaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgt gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acaccccctt tgaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcggac agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaggggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gccttatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgttgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaattagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgtcttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcgacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcta
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctggtgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccag gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gctgtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttagacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtgccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgtcgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg cgagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aagctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgcat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatctc tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttga ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtactgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgccccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgccagtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatag tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg caggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 tcggtaatgc tgccaactta ctgatttag
//
LOCUS       DADMEQ010000066        20481 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.066, whole genome shotgun sequence.
ACCESSION   DADMEQ010000066 DADMEQ010000000
VERSION     DADMEQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.066"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            145..297
                     /gene="hokA"
                     /locus_tag="JNZ05_002428"
     CDS             145..297
                     /gene="hokA"
                     /locus_tag="JNZ05_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HAY8565633.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(484..696)
                     /gene="cspA"
                     /locus_tag="JNZ05_002429"
     CDS             complement(484..696)
                     /gene="cspA"
                     /locus_tag="JNZ05_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HAY8565634.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNYGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(977..1267)
                     /locus_tag="JNZ05_002430"
     CDS             complement(977..1267)
                     /locus_tag="JNZ05_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HAY8565635.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIERLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLMK"
     gene            1701..2411
                     /locus_tag="JNZ05_002431"
     CDS             1701..2411
                     /locus_tag="JNZ05_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HAY8565636.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2461..3435)
                     /gene="ghrB"
                     /locus_tag="JNZ05_002432"
     CDS             complement(2461..3435)
                     /gene="ghrB"
                     /locus_tag="JNZ05_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HAY8565637.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3539..4198)
                     /locus_tag="JNZ05_002433"
     CDS             complement(3539..4198)
                     /locus_tag="JNZ05_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HAY8565638.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4351..6684
                     /locus_tag="JNZ05_002434"
     CDS             4351..6684
                     /locus_tag="JNZ05_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY8565639.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLAHCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6653..7093)
                     /locus_tag="JNZ05_002435"
     CDS             complement(6653..7093)
                     /locus_tag="JNZ05_002435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY8565640.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7090..7653)
                     /gene="tag"
                     /locus_tag="JNZ05_002436"
     CDS             complement(7090..7653)
                     /gene="tag"
                     /locus_tag="JNZ05_002436"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAY8565641.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKCGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7811..8509
                     /locus_tag="JNZ05_002437"
     CDS             7811..8509
                     /locus_tag="JNZ05_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY8565642.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDSTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8738..9946
                     /locus_tag="JNZ05_002438"
     CDS             8738..9946
                     /locus_tag="JNZ05_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8565643.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10270..11961
                     /gene="eptB"
                     /locus_tag="JNZ05_002439"
     CDS             10270..11961
                     /gene="eptB"
                     /locus_tag="JNZ05_002439"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HAY8565644.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12053..12129
                     /locus_tag="JNZ05_002440"
     tRNA            12053..12129
                     /locus_tag="JNZ05_002440"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12087..12089,aa:Pro,seq:cgg)
     gene            12873..14480
                     /gene="dppA"
                     /locus_tag="JNZ05_002441"
     CDS             12873..14480
                     /gene="dppA"
                     /locus_tag="JNZ05_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HAY8565645.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14788..15807
                     /gene="dppB"
                     /locus_tag="JNZ05_002442"
     CDS             14788..15807
                     /gene="dppB"
                     /locus_tag="JNZ05_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HAY8565646.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15817..16719
                     /gene="dppC"
                     /locus_tag="JNZ05_002443"
     CDS             15817..16719
                     /gene="dppC"
                     /locus_tag="JNZ05_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HAY8565647.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16730..17713
                     /gene="dppD"
                     /locus_tag="JNZ05_002444"
     CDS             16730..17713
                     /gene="dppD"
                     /locus_tag="JNZ05_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HAY8565648.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17710..18714
                     /gene="dppF"
                     /locus_tag="JNZ05_002445"
     CDS             17710..18714
                     /gene="dppF"
                     /locus_tag="JNZ05_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HAY8565649.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18744..20015)
                     /locus_tag="JNZ05_002446"
     CDS             complement(18744..20015)
                     /locus_tag="JNZ05_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY8565650.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4906 a         5070 c         5381 g         5124 t
ORIGIN      
        1 tccaattgaa tatcggaatc agacctatat gcctcgtgtt taactgtcca actttttggg
       61 gtcagtacaa agtgaggcta ccctatgcct gaacaccata aggtgtgcct cttactcgtt
      121 ggaaatcaac acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg
      181 atttgtttca cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat
      241 attaaacagg agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag
      301 tcttcggcgg gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccac
      361 atttattctt cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga
      421 aaaaaaataa aaaaatcccc gcaaaatggc agggatctta gattctgtgc ttttaatcag
      481 agattacagg ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa
      541 ggacactttc tgaccttcgt ccagagattt gtaaccatag ttctggatag cagagaagtg
      601 tacgaacaca tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt
      661 gaaccatttt acgataccag tcattttacc ggacatagtg tattaccttt aataattaag
      721 tgtgcctttc ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact
      781 acgaggggta tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc
      841 tgtacgtcaa accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt
      901 tttcatattt atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa
      961 taaagtggat aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca
     1021 aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta
     1081 ccatggcgac tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt
     1141 ttcgtaaccg ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg
     1201 tttcatcttt aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat
     1261 attccattga aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta
     1321 agaatagtcg ggaaaagcag acacagcggg gttaagtcag ggcatgctct ggctatgatt
     1381 cgattcagcc tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata
     1441 tatctcttat cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta
     1501 aatctctggc tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt
     1561 aaccttgttt cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc
     1621 aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt
     1681 tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc
     1741 ttgcggcgtt attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc
     1801 gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa
     1861 ccctgactgc cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt
     1921 atggttattc tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca
     1981 gcgttaacgc aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa
     2041 tgaatggttc gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg
     2101 ctgcgcactc tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca
     2161 ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt
     2221 tcacgcaaca actggtgatg gttggagact acattgctca gcaaggtact caggtgagct
     2281 ttgtcgcaaa tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga
     2341 ttgcgccatt accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg
     2401 caacccaata aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcggc
     2461 ttagtccgcg acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat
     2521 caaattatcc acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagagccaat
     2581 atgcggtact gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc
     2641 ttgttcgaag acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag
     2701 tgcattttcg tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat
     2761 tttggcgaat tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag
     2821 gcaaacgaaa tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg
     2881 ttcttctgct tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa
     2941 gtgcgcacgt tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt
     3001 atggtgaacg tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt
     3061 tacccgttct gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt
     3121 atcggcgacg gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt
     3181 aagcgcatcg acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt
     3241 cggcattttt tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc
     3301 ttctgcaaaa attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg
     3361 aacggtgaaa tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat
     3421 aacggacggc ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata
     3481 tgatgaagga aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt
     3541 acagcgggct taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt
     3601 tgctggcgat tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc
     3661 cctgggtgat caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca
     3721 ggtcgtgacc ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact
     3781 ctttcagcac cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggcgcgc
     3841 tgctgctgtc gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg
     3901 ttacgctaac accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt
     3961 aataacccac gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac
     4021 cgcgatcttt cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac
     4081 ctgcgccgat agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc
     4141 cagataccgc cagagcgcca ctcactacgg cggcaataag ataaacacgt ttcttcattg
     4201 ttaatcctta ataacctttt tattctttgc cacgggttcc gtggcgggag attatgccgc
     4261 gtgaacatga agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc
     4321 aatacatcta acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt
     4381 ctgactgccg cccactgggg gcccatgctg gttgaaaccg acggcgaaac cgtgtttagc
     4441 tcgcgtggcg cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag
     4501 gttcacagca atacgcgggt acgatttcca atggtgcgca aaggctttct cgcgtcaccg
     4561 gaaaacccgc aaggcattcg tgggcaggat gaatttgttc gcgtgagttg ggatgaggcg
     4621 ctggatctta ttcaccaaca acataaacgc attcgtgagg cttatggtcc ggcatcgatt
     4681 tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta
     4741 caacgctata tggcgctggc aggcggttat accgggcatc tgggggatta ttcgaccggt
     4801 gcggcacagg cgatcatgcc gtatgtcgta ggtggcagcg aagtttatca acagcaaacc
     4861 agttggccgc tggtgctgga acatagcgat gtcgtggtgt tgtggagtgc taacccactc
     4921 aatacgctga aaattgcgtg gaatgcatcc gatgagcagg ggctttctta cttttccgca
     4981 ctgcgtgaca gtgggaaaaa gctgatctgc attgatccaa tgcgatcgga aaccgtcgat
     5041 ttctttggcg ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt tgcgctgatg
     5101 ctggggatcg cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcat
     5161 tgcaccacag gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggaatagcg
     5221 aaaaacgccg aatgggcggc agagatttgt ggtgttggcg cagcgaaaat ccgcgagctg
     5281 gcggctattt tccaccaaaa taccaccatg ctgatggcag gctggggaat gcagcgccaa
     5341 cagtttggtg agcaaaaaca ctggatgatc gtcacgctgg cagcaatgtt ggggcaaatc
     5401 ggcacacccg gcggcggttt tggtctttct taccattttg ccaatggtgg taaccccacg
     5461 cggcgttctg cggtgctctc ttccatgcag ggcagcttgc cgggtggctg cgatgcggtg
     5521 gataaaatcc ctgttgcccg cattgttgaa gcactggaaa accctggtgg cgcatatcaa
     5581 cacaacggta tgaaccgaca tttcccggat attcgtttta tctggtgggc gggcggtgcc
     5641 aactttactc atcatcagga taccaatcgt ctgatccgtg cctggcaaaa accggagctg
     5701 gtggtgatct ctgaatgctt ctggacggcg gcggcgaaac acgcggatat cgttctgcct
     5761 gcgactacct cttttgagcg taatgatctc accatgaccg gtgattacag taatcagcat
     5821 ctggtgccga tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga ttttgatgtt
     5881 tttgccgagt tgagtgaacg ttgggagaag ggcggctatg cgcggtttac ggaaggaaaa
     5941 agtcagctgc aatggctgga aacgttttat aacgttgccc ggcagcgcgg ggcaagccag
     6001 caggttgaat tgccgccatt tgctgagttc tggcaagcca accagttaat tgagatgccg
     6061 gaaaacccgg acagcgagcg gtttattcgc ttcgctgatt tttgccgcga tccgctggcg
     6121 catccgttaa aaaccgccag cggcaagatt gaaatcttct cacagcgtat tgccgattac
     6181 ggttacccgg attgccctgg gcatccaatg tggctggagc cggacgaatg gcagggcaat
     6241 gccgaaccag aacagttgca ggtactttct gcccatccgg cgcaccgcct gcacagccag
     6301 ctgaattaca gttctctgcg cgaattgtac gcggtagcaa accgtgagcc tgtcaccatt
     6361 catcctgacg atgctcaggc gcgcggcata accgaagggg atatggtgcg ggtgtggaac
     6421 tcccgtgggc agatccttgc tggagcggtc attagcgagg gaattaaacc tggcgtgatt
     6481 tgcattcacg aaggggcatg gccggatctg gatttaaccg ctgacggtat ttgtaaaaac
     6541 ggcgcagtga acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg
     6601 ggtaatacgg cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg
     6661 tttgaaccac cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc
     6721 aatcgacaat gtgaaaaccc tgcgcacggt aaaaatctat cgccggttga tttttttgat
     6781 aaacctccag catcagatgg ggatagcgct gctgcacata ctgcatcagc gccttaccaa
     6841 taccgcgtct gacggccttc ggtgcgacaa acattgctgc cagaaatcgg ccttccataa
     6901 tgctgacaaa accgagaagc ttaccgtcct cttcccagac ccagttttgc gcgttggcaa
     6961 gataggcatc ccgcaccagc ggaatgcagt cacgccagta attcgctttt ataaagggat
     7021 gcccccaggt tgtactttcc agccacagtt cgaggatcgc ggggagttct gaacgttgcg
     7081 cttcccgaat catggtttat ttcccggata gcaacagcag ccaaccacat gatcattcac
     7141 cagcccacat gcctgcataa aagagtaaca gattgtggtg ccgacaaact taaaaccaca
     7201 ttttttcagt gccttagata gggcgtcgga ggcggacgta gatgtgggaa tttcgctcaa
     7261 cgttgtggct tgtgtcacct gtggctgatg atttacaaac gaccagacaa agtcgggaaa
     7321 cggttcgccg ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat
     7381 tttccctcga tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg
     7441 cattgctgcg accttcaccg gatcgaactg atgaaagcag gcgcgatagt tttcgcgttt
     7501 tttgaggacg gtgatccacg ataacccagc ctgctgccct tcaaggcaga tcatttcgaa
     7561 cagtttttta ctgtcagttt caggcacgcc ccactcatta tcatggtagg caatataaag
     7621 cgggccctga ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt
     7681 aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct
     7741 actatgcccg atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg
     7801 agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgctggcgag
     7861 cgtggtaatc agtgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt
     7921 tgatacgcaa cccggacatt ccacagagcg ttacacctgg gatagtgatc atcaacctga
     7981 ttacaacgat attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt
     8041 caatctggcg gaagaaactc ctgtggatgt gaccagcagt atgagtatgg gctggaattt
     8101 tcctttatat gaacaggtta caaccggccc ggtcgcggca ttacattacg atagcacaac
     8161 cacctcgatg tataacgagt ttggcgacag tactaccacg ctgaccgatc cgttatggca
     8221 tgccagcgtg agtagcttag gctggcgtgt tgactcccgg cttggcgatc tccgaccctg
     8281 ggcgcaaatc agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct
     8341 gagccggatg acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga
     8401 tatgttgctc aatcaaaata ttgccgccta tgctgcgcta actcaggcag aaaataccac
     8461 taataatagc gactatctgt atacgatggg ggttagcgcc agattttaac gtaacagtca
     8521 caattgaaac cattgaataa caacagttgt ggcgatagtg ggtgctaact taccaaataa
     8581 taaatttggt gaataattgt cgcgtcattc attcctgaac taaggcattt cattccgttc
     8641 tgatggcatt tcatgccgtt tttccccaag cataaaatgc acttcgttat ggttgtcggc
     8701 agagattttt cctttttatt actgcaggaa tactgccatg acaccttcaa attatcagcg
     8761 tacccgctgg ctgacactca tcggcactat cattacccag tttgcgctgg ggtcggttta
     8821 tacctggagc ctgtttaatg gcgcgctttc tgccaagctg gatgcgccgg taagccaggt
     8881 cgctttctct ttcggcttgt taagtctggg gctggcaatt tcgtcttctg ttgcgggcaa
     8941 attgcaggaa cgttttggcg ttaaacgcgt caccgtggct tccggcattt tgctgggatt
     9001 aggcttcttc ctgaccgcgc attccaacaa cctgatgatg ctgtggttaa gcgccggtgt
     9061 gctggtcggt ctggcggatg gcgcgggtta cctgctgacg ctctctaact gcgtgaagtg
     9121 gttcccggag cgtaaaggtc tgatctccgc gttcgctatc ggttcttatg gtctgggtag
     9181 cctgggtttc aaatttatcg acacgcagct gctcgaaacg gtgggtctgg aaaaaacctt
     9241 tgtgatttgg ggagcgattg cgctggtgat gattgttttc ggcgcaacgt taatgaaaga
     9301 cgcaccaaaa caggaagtga aaaccagcaa tggtgtggtg gagaaagatt acacgctggc
     9361 agagtcgatg cgtaaaccgc agtactggat gttagcggta atgttcctga ctgcctgcat
     9421 gagcggcctg tacgtgattg gggtagcgaa agatatcgcc caaagtctgg cacaccttga
     9481 tgtggtttcc gcagccaatg cagtaacggt tatttccatc gctaaccttt caggtcgtct
     9541 ggtgctgggc attctgtctg acaaaattgc ccgtatccgt gttatcacca ttggtcaggt
     9601 gatttcgctg gtgggtatgg cggccctgct gtttgcacca ttaaatgcag tgacgttctt
     9661 tgcagcgatt gcctgcgtgg catttaactt tggcggcact attaccgtct ttccatcact
     9721 ggtcagtgag ttcttcggcc tcaataacct ggcgaaaaac tacggtgtga tttacctcgg
     9781 tttcggtatc ggtagcattt ttggttcgat tatcgcctca ctgtttggcg gtttctatgt
     9841 gaccttctac gtgattttcg ccctgctgat tctgtcattg gcgctttcta cgacgattcg
     9901 tcagccagag cagaaaatgt tgcgtgaggc gcatggctcc ctttaatgct cagccgcgag
     9961 ctactgggct tcagcttccg tgtgcatcaa acgcatcaac gaaatgtata aacaatgact
    10021 cgtaaaattt gggccagtta actggccctt tttactgctt tttgtaacgc taaagtctct
    10081 tttcaaactt gcatttttgt aaatttgtgc ttcatgcaca ctctttcccc acactttttc
    10141 gctttgctgt ggtctactta ttcgcgcgtg tagattttac ttatctgact acctccgcac
    10201 ttttcccctg ccgggcctga aaagccgcta agcagggtgt tatcacctgt ttgtccaggg
    10261 tttgtttgca tgagatacat caaatcgatt acacagcaga agctgagctt tttgcttgca
    10321 atctatattg gcctttttat gaatggcgcg gttttttatc gccgcttcgg cagctatgcg
    10381 cacgatttta ccgtctggaa aggcgtttct gctgttgttg aactggccgc caccgtactg
    10441 gtgaccttct ttttactacg tcttctatcg ctgtttggcc gccgcagctg gcgtattctg
    10501 gcgtcgctgg tggtgctctt ttccgcaggt gccagctatt acatgacctt ccttaatgtg
    10561 gtcattggtt atggcatcat cgcttccgtc atgaccaccg atatcgacct gtcaaaagaa
    10621 gttgttggtc tgaactttat tctctggtta atcgccgtta gtgcattgcc tcttatcctt
    10681 atctggaata accgctgtcg ctacaccttg ctccgacaac tgcgaacccc agggcagcgt
    10741 attcgcagcc tggtggtcgt cgtactggcg ggtattatgg tttgggcacc gattcgtttg
    10801 ctggatatcc agcagaagaa agtggagagg gcgaccggcg ttgatttgcc gagttatggc
    10861 ggtgtcgtag cgaactctta tctgccatca aactggcttt ctgcgttggg gctgtatgcc
    10921 tgggcgcggg tcgatgaatc ttccgataat aattcattgc ttaatccggc gaagaagttt
    10981 acataccagg ctccacaaaa cgttgatgat acttatgtcg tgtttatcat cggtgaaacc
    11041 acgcgttggg accatatggg tattttcggc tatgagcgta ataccacgcc gaaactggcc
    11101 caggagaaaa atctggcggc gttccgtggt tactcctgtg ataccgcaac caaactctca
    11161 ctgcgttgca tgtttgtacg tcaggggggc gcggaagata atccgcagcg cacattaaaa
    11221 gaacagaaca ttttcgcggt tctgaagcag ttaggattca gttctgacct ctacgctatg
    11281 cagagcgaaa tgtggttcta cagcaacacg atggcagaca acattgctta tcgtgagcag
    11341 attggtgcgg agccacgtaa tcgtggcaag ccggtagatg atatgttgct ggtagacgaa
    11401 atgcagcaat cgctagggcg caacccggat ggtaagcatc tgatcattct gcataccaaa
    11461 ggttcgcatt ttaactacac ccagcgttat ccgcgtagct tcgcgcagtg gaagccggaa
    11521 tgtattggtg ttgatagcgg ctgtaccaaa gcgcagatga tcaactccta tgacaactcg
    11581 gtgacctatg tggatcactt tatctccagc gtgattgatc aggttcgcga taagaaagcg
    11641 attgtgttct acgcagctga ccacggtgag tcaattaatg aacgcgagca cctgcacggc
    11701 acgccgcgtg aactggcacc gccggagcag ttccgcgtac cgatgatggt ctggatgtca
    11761 gataaatatc tggaaaatcc ggccaatgcg caggcgtttg cgcagctgaa aaaagaagcc
    11821 gacatgaaag tgccacgccg tcacgtagag ctgtacgaca ccatcatggg ttgtcttggc
    11881 tatacttcac cggatggtgg aattaacgaa aacaacaact ggtgtcacat cccgcagaca
    11941 aaagtggcag cggctaacta agcggcctgc tgactttctc gccgatcaaa aggcattttg
    12001 ctattaaggg attgacgagg gcgtatctgc gcagtaagat gcgctccgca ttcggtgatt
    12061 ggcgcagcct ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg aatcctctat
    12121 caccgaccaa attcgaaaag cctgctcagc gagcaggttt ttttgcattt gtagctcatg
    12181 aggatgagaa cctccggggg cagggaggct cgactcgacg taggcctgat aagacgcgcc
    12241 agcgtcgcat caggcaacgg caccgcactt attgtcggat gcggcgcgac cgccttattc
    12301 gaccaacaca ccacacattt tcctgccaac tttgcgcccc atatctcact atccattaac
    12361 ggatttgtga cagaaaccat tgtctttttt tatatttgct taaatctttt ttgcgttact
    12421 tatggctggc gctggcattt tccgctgtgc gttttagcgt tcatggtatt aatcgctcag
    12481 atatccacct tttcctcaga tttttttcat taaatgcata aaacttaatc accaaatttt
    12541 gctaaatatt aagctgattg ttctggttat gttggagtag cacaaatttc tctgctgcaa
    12601 atagctgttt gaagtttttt taatctttgt ttgtggattc tcaccgttgg tgtaaatccc
    12661 ggttggctgt attgacgttt tcacattctg ttgacagatt gtaggtcacg aggggcattt
    12721 tatggaggat ccgcactgtt acactgatgt taattagtac ggcatcccca cctcataacg
    12781 ttgacccgac cgggcaaaaa acaaaaaagg tcaggcagcg acaacccact gcaaagggtt
    12841 aaaacaacaa acatcacaat tggagcagaa taatgcgtat ttccttgaaa aagtcaggga
    12901 tgctgaagct tggtctcagc ctggtggcta tgaccgtcgc agcaagtgtt caggctaaaa
    12961 ctctggttta ttgctcagaa ggatctccgg aagggtttaa cccgcagctg tttacctccg
    13021 gcaccaccta tgacgcctct tccgtcccgc tttataaccg cttggttgaa tttaaaatcg
    13081 gcaccaccga agtgatcccg ggcctcgctg aaaagtggga agtcagcgaa gacggtaaaa
    13141 cctatacctt ccatctgcgt aaaggtgtga agtggcacga caataaagaa ttcaaaccga
    13201 cgcgcgaaat gaacgccgat gacgtggtgt tctcgttcga tcgtcagaaa aacgcgcaaa
    13261 acccgtacca taaagtttct ggcggcagct acgaatactt cgaaggtatg ggcttgccgg
    13321 agctgatcag cgaagtgaaa aaggtggacg ataacaccgt tcagtttgtg ctgactcgac
    13381 cggaagcgcc gttcctcgct gacctggcaa tggacttcgc ctctattctg tcaaaagaat
    13441 atgctgacgc gatgatgaaa gccggtacac cggaaaaact ggacctcaac ccaatcggaa
    13501 ccggtccgtt ccagttacag cagtaccaaa aagattcccg tatccgctac aaagcgtttg
    13561 atggctactg gggcaccaaa ccgcagatcg atacgctggt cttctctatt acccctgatg
    13621 cttccgtgcg ttacgcgaaa ttgcagaaga atgaatgcca ggtgatgccg tacccgaacc
    13681 cggcagacat cgctcgcatg aagcaggata aatccatcaa cctgatggaa atgccggggc
    13741 tgaacgtcgg ctacctctcg tataacgtgc agaaaaaacc gctggatgac gtgaaagttc
    13801 gccaggcact gacctacgcg gtgaacaaag acgcgatcat caaagcggtt tatcagggcg
    13861 cgggtgtatc agcgaaaaac ctgatcccgc caaccatgtg gggctataac gacgacgttc
    13921 aggattacac ctacgatcct gaaaaagcga aagccttgct gaaagaagcg ggtctggaaa
    13981 aaggtttctc catcgacctg tgggcgatgc cggtacaacg tccgtataac ccgaacgctc
    14041 gccgcatggc ggagatgatt caggcagact gggcaaaagt gggcgtgcag gccaaaatcg
    14101 tcacctacga atggggtgag tacctcaagc gtgcgaaaga tggcgaacat cagacggtaa
    14161 tgatgggctg gactggcgat aacggggacc cggataactt cttcgccacc ctgttcagct
    14221 gcgccgcctc tgaacaaggt tctaactact caaaatggtg ctacaaaccg tttgaagatc
    14281 tgattcaacc ggcgcgtgct accgacgacc ataacaaacg cgttgaactg tacaagcaag
    14341 cgcaggtggt gatgcacgat caggctccgg cactgatcat cgctcactcc accgtgtttg
    14401 aaccggtacg caaagaagtc aaaggctatg tggttgatcc attaggcaaa catcacttcg
    14461 aaaacgtctc tatcgaataa ttaaaagcca tacaagactg atggcaaagg caaaactgcc
    14521 tgatgtgctc cgcttatcag gcctacgaaa attctgcaat gtattgaatt tgcacgattt
    14581 tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caaagcgcac tttgtcaaca
    14641 atctgtatac ccggtggcgc tgtgcctctg cgtgcagcgc catccggcag caatacttca
    14701 ttctctgtcc gacaagggcg ggaattgatt tgtgagcaat acagacacgc agttccaggc
    14761 tgcgggtcac tatagagaat ccgggttatg ttgcagttta ttctccgacg tttgggactc
    14821 gtcatcccca cgtttatcgg tattaccctt ctcacatttg cctttgtcca catgatcccg
    14881 ggcgatccgg tgatgatcat ggcaggcgaa cgtgggatct cccctgagcg tcacgtgcag
    14941 ctgctggctg aactcggctt agataaaccg atgtggcagc agtatctcca ttacatttgg
    15001 ggcgtaatgc acggcgatct aggcatttca atgaaaagcc gaattccagt atgggaagag
    15061 ttcgtgccgc gctttcaggc cacgctggaa cttggcgtct gcgcgatgat ttttgctacg
    15121 gcagtcggta ttccggttgg tgtgctggct gcggttaaac gcggttccat tttcgatcac
    15181 actgcagttg gcctggcgct gaccggctac tcgatgccga tcttctggtg gggcatgatg
    15241 ctgatcatgc tggtttcggt gcactggaac ctgacgcccg tctccggtcg cgtgagcgat
    15301 atggtgttcc tcgatgactc caatccgtta accggtttta tgctaatcga caccgccatc
    15361 tggggtgaag acggcaactt tatcgatgcc gtcgcccata tgatcttgcc cgccattgtg
    15421 ctgggtacta ttccgctggc ggtcattgtg cgtatgacac gctcctcgat gctggaagtg
    15481 ctgggcgagg attacatccg caccgcgcgc gccaaagggc taacccgcat gcgggtgatt
    15541 atcgtccatg cgctgcgtaa cgcgatgctg ccggtagtga ccgttatcgg cctccaggtg
    15601 ggaacattgc tggcgggggc gattctgacc gaaaccatct tctcgtggcc cggtctgggg
    15661 cgctggttga ttgacgcact gcaacgccgc gactatccgg tagtgcaggg cggcgtattg
    15721 ctggtggcga cgatgattat cctcgtcaac ttgctggtcg atctgctgta cggcgtggtg
    15781 aacccgcgta ttcgtcataa gaagtaaggg gacatcatgt cacaggttac tgaaaataaa
    15841 gtgattagcg caccggtgcc gatgaccccg ttacaggagt tctggcacta ttttaaacgc
    15901 aacaaaggcg cggtcgtcgg gctggtttac gtcgccatcg tgctgttcat cgcgatcttt
    15961 gccaactgga ttgcgcctta taacccggcg gatcagttcc gcgatgcgct gctcgccccg
    16021 ccagcctggc aggaaggcgg cagtatggca cacttgctgg gcaccgatga cgtcggtcgt
    16081 gatgtgctgt cgcggctgat gtacggcgcg cgtttatcgc tgctggttgg ctgtctggtg
    16141 gtggtgttgt cgctgattat gggcgttatt ctcggcctga tcgccggtta cttcggcggc
    16201 ctggttgata acattatcat gcgcgtggtc gatatcatgc tggcgctgcc aagtctgctg
    16261 ttggcactgg tgctggtggc gattttcggc ccgtcgattg gtaacgccgc gctggcgctg
    16321 accttcgttg ccttgccgca ctatgtgcgc ttaacccgcg ccgccgtgct ggtggaggtg
    16381 aatcgcgatt acgtcaccgc ctctcgcgtg gcaggtgccg gggcgatgcg ccagatgttt
    16441 attaacatct tcccgaactg ccttgcgccg ctgattgttc aggcgtcgct cggtttctct
    16501 aacgccattc tcgatatggc tgctcttggt ttcctcggca tgggggcaca gccgccaacg
    16561 cctgagtggg gcaccatgct ctccgacgtg ttgcagttcg cgcaaagcgc ctggtgggtc
    16621 gtgaccttcc ctggtctggc gatcctgttg acggtgctgg catttaacct gatgggtgac
    16681 ggtctgcgtg acgcgctcga tcccaaactg aagcagtaag aggttcgaga tggcgttatt
    16741 aaatgtagat aaattatcgg tgcatttcgg cgacgaaagc gcaccgttcc gcgccgtaga
    16801 ccgcatcagc tacagcgtaa aacagggcga agtggtcggg attgtgggtg agtccggctc
    16861 cggtaagtcg gtcagttcac tggcgattat ggggctgatt gattatccgg gccgcgtaat
    16921 ggcagaaaaa ctggagttta acggccagga tttgcagcgt atctcagaaa aagagcgccg
    16981 caacctggtg ggtgccgaag tggcgatgat cttccaggac ccgatgacca gccttaaccc
    17041 atgctacacc gtgggtttcc agattatgga agcgattaaa gtgcatcagg gcggcaacaa
    17101 aagtacccgc cgtcagcggg cgattgacct gctgaatcag gtcggtattc ccgatccggc
    17161 ttcgcgtctg gatgtttacc cgcatcagct ttccggcggc atgagccagc gcgtgatgat
    17221 cgccatggcg attgcctgtc ggccaaaact gctgattgcc gatgaaccga ccaccgcgct
    17281 ggacgtgacc attcaggcgc aaatcatcga actactgctg gagctacagc agaaagagaa
    17341 catggcgctg gtgttaatta cccatgacct ggcgctggtg gcggaagctg cgcataaaat
    17401 cattgtgatg tatgcaggtc aggtagtaga aacgggcgat gcacacgcca tcttccatgc
    17461 gccgcgtcac ccgtatactc aggcattgct gcgtgcgctg ccagaatttg ctcaggacaa
    17521 agaacgtctg gcgtcgttgc caggtgttgt tcccggcaag tatgaccgcc cgaacggctg
    17581 cctgcttaac ccgcgctgcc cctatgccac tgacagatgt cgcgctgaag aaccggcgct
    17641 gaatatgctc gctgatgggc gtcagtccaa atgccattac ccacttgatg atgccgggag
    17701 gcccacacta tgagtacgca agaggccacc tcgcaacaac cgctgttgca ggctatcgac
    17761 ctgaaaaaac attatccggt gaagaaaggc atgttcgtgc cggaacgtct ggtgaaggcg
    17821 ctggacggcg tttcgtttaa ccttgaacgt ggcaaaacgc tggcagtagt gggcgaatct
    17881 ggctgcggta aatcgaccct cggtcggttg ctgacgatga tcgaaacgcc caccggcggc
    17941 gagctgtatt atcaggggca ggatctgctc aagcacgatc cgcaggcgca gaagctgcgt
    18001 cggcagaaaa tccagatcgt cttccagaat ccctacggtt cgctgaatcc gcgtaagaaa
    18061 gttgggcaaa ttctcgaaga gccgctgctt attaacacca gcttaagcaa agaacagcgt
    18121 cgggaaaaag ccctgtcgat gatggcaaaa gtcggcctga aaaccgagca ctatgaccgc
    18181 tatccgcaca tgttttccgg cggacaacgt cagcgtatcg ctattgcccg tggtctgatg
    18241 ctcgacccgg atgtggtgat tgccgatgag ccggtttctg cgctggacgt ctcagtgcgt
    18301 gcgcaggtgc tgaatctgat gatggatttg cagcaggagt tggggctttc ttatgtcttt
    18361 atctcccacg acctgtcggt tgtggagcac attgctgatg aagtgatggt gatgtaccta
    18421 ggccgctgcg tggagaaagg aacgaaggac caaatcttca ataacccgcg ccatccgtac
    18481 acccaggcgc tgctctccgc gacgccacgc ctgaacccgg acgatcgtcg tgaacgcatc
    18541 aaactcaccg gtgaactgcc aagcccgctg aatcctccgc cgggttgcgc cttcaacgcc
    18601 cgctgtcgtc ggcgcttcgg cccctgcacc cagttgcagc cgcagctaaa agactacgga
    18661 ggtcaactgg tagcttgttt tgctgttgat caggatgaaa atccgcagcg ttaactcaca
    18721 aaaccggggc catgcgctcc ggtttactca atcagcttca acagtggcga caagcatagc
    18781 aaaatgccgt aaagcagaat cagccaggct ttaaatccgc gaagtttttc cagttgtgcg
    18841 actttataga tcaggaaaaa cggaatgagg cacgacacaa tgccatataa cgggcttccc
    18901 aactgaaaga acaccagcac tgatacacga aacgaaaccc aaatcgtcaa cgtaatgacg
    18961 ataaaagcgc agatcgccag agtcagcatg cgtgagttaa ttttcttggt atcaataatt
    19021 cggcttaaca gattgagaat aatgccttta atagcctcgt ggaaaccgag ataaatgcca
    19081 aagaatgcgg tcagtacggc aaagatattc agcaccgtag aggtgatatg aatgatatgt
    19141 ccagggatca cctgcgcggc cagtgccagt gccgagatat tttgttcaaa ggcagaaacg
    19201 gcttcttcgt ggctaattga gaaggtaaac gaaaaggcaa aaaacaggat caccgcgatg
    19261 agcgtgatat aactaattcg gtgggtacgc agcgcgagcc gggttgccag tactttatct
    19321 gcttcccgtt tacgataggc aatattcatt gggttaagta cctgaataaa taccgcagaa
    19381 aagaagcaaa atggaatggt aagcaggaca tcgcggaaaa agacggaggc ttgcgggaag
    19441 gcggttatat tggcgaaatt ccagtgcggg atcatcgcaa aaccgaacac gacaataatc
    19501 cctactttga ccaccaccat tggcccagaa atcttaaaca gtaatcgttc accaccagac
    19561 gcaatcgcta tcagtacggc gaaaatagcg actttataaa gtagagattg tgaaagatcg
    19621 gcatcggtta aaccgaaggt tttcaggtac gaggcgctgt cgaaaaccac ggagagagag
    19681 tagataaaaa tcccgtggat aatcatcaaa aagtagataa ccccgaggaa aattccccag
    19741 ttcttcccca ggtaatgact gataatatcg gtgtagtcat tacaggaatc gctttcagaa
    19801 agggttttta aataaatgtc ctgcactacc caggtggcag gataagcaat gatcgctgcg
    19861 gtaataaata cccaaattcc cttcaagcca atttgtactg gcatcagcac ggttccggca
    19921 ccaatcgcca tgcctatgca taataaaacc cagccgaagt cgtagcgtgt aaacggcaat
    19981 ttctgattgt gtttcgatag tgtgttgtgc tgcataaagt cctgtccaga aagaagtcgg
    20041 cctcgcaaaa atgaataaac ccagtttctc ttgcgcaaca ggtggagaag tcgaatacgc
    20101 agtatgtaaa aaatgaggca acagattcac tggggaggtc gcgaggaggc gcaattctac
    20161 aaaattgcgc atttcactac cagtctggag tcagcccgga tatgaactgc aactattgca
    20221 aataaagaaa ttaagctgga aggcatcgcg caatgaggaa gattgccgga aaaagcaatc
    20281 gtaaaatcag cggcaagccg gaagggttct ggtgaggcgc aatgttgcgg gggctttatc
    20341 cctggtggca ttggttgctg gagagagaaa acccccgcac gttgcaggta tgtacctgac
    20401 aacataacgg gggctaatct tgaacctaga ccactcaaga atagccgcga aacgttgtca
    20461 ttacaacaca ggcggctata t
//
LOCUS       DADMEQ010000067        19929 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.067, whole genome shotgun sequence.
ACCESSION   DADMEQ010000067 DADMEQ010000000
VERSION     DADMEQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.067"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..441
                     /gene="feaR"
                     /locus_tag="JNZ05_002447"
     CDS             <1..441
                     /gene="feaR"
                     /locus_tag="JNZ05_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HAY8565651.1"
                     /translation="QESMNNPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQ
                     KVVTLIDDNIREEILRPEWIAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAI
                     RHAADDEKLAGIGFHWGFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(613..942)
                     /locus_tag="JNZ05_002448"
     CDS             complement(613..942)
                     /locus_tag="JNZ05_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HAY8565652.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(947..1132)
                     /locus_tag="JNZ05_002449"
     CDS             complement(947..1132)
                     /locus_tag="JNZ05_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HAY8565653.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(1129..3768)
                     /locus_tag="JNZ05_002450"
     CDS             complement(1129..3768)
                     /locus_tag="JNZ05_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HAY8565654.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLDVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHVGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3976..4965
                     /gene="ldhA"
                     /locus_tag="JNZ05_002451"
     CDS             3976..4965
                     /gene="ldhA"
                     /locus_tag="JNZ05_002451"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY8565655.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAVLELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            5076..5412
                     /locus_tag="JNZ05_002452"
                     /pseudo
     CDS             5076..5412
                     /locus_tag="JNZ05_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
     gene            complement(5409..5675)
                     /locus_tag="JNZ05_002453"
     CDS             complement(5409..5675)
                     /locus_tag="JNZ05_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HAY8565656.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5949..9473
                     /gene="nifJ"
                     /locus_tag="JNZ05_002454"
     CDS             5949..9473
                     /gene="nifJ"
                     /locus_tag="JNZ05_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAY8565657.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAGAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9840..10969
                     /gene="ompN"
                     /locus_tag="JNZ05_002455"
                     /pseudo
     CDS             9840..10969
                     /gene="ompN"
                     /locus_tag="JNZ05_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            11110..11544
                     /gene="uspF"
                     /locus_tag="JNZ05_002456"
     CDS             11110..11544
                     /gene="uspF"
                     /locus_tag="JNZ05_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HAY8565658.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11720..12655
                     /gene="ttcA"
                     /locus_tag="JNZ05_002457"
     CDS             11720..12655
                     /gene="ttcA"
                     /locus_tag="JNZ05_002457"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAY8565659.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(12787..14154)
                     /gene="dbpA"
                     /locus_tag="JNZ05_002458"
     CDS             complement(12787..14154)
                     /gene="dbpA"
                     /locus_tag="JNZ05_002458"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HAY8565660.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(14632..15615)
                     /gene="zntB"
                     /locus_tag="JNZ05_002459"
     CDS             complement(14632..15615)
                     /gene="zntB"
                     /locus_tag="JNZ05_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HAY8565661.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIISTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            15870..17102
                     /gene="dgcM"
                     /locus_tag="JNZ05_002460"
                     /pseudo
     CDS             15870..17102
                     /gene="dgcM"
                     /locus_tag="JNZ05_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(17123..17686)
                     /gene="smrA"
                     /locus_tag="JNZ05_002461"
     CDS             complement(17123..17686)
                     /gene="smrA"
                     /locus_tag="JNZ05_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HAY8565662.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(17940..19394)
                     /locus_tag="JNZ05_002462"
     CDS             complement(17940..19394)
                     /locus_tag="JNZ05_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAY8565663.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGILQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(19391..19843)
                     /locus_tag="JNZ05_002463"
     CDS             complement(19391..19843)
                     /locus_tag="JNZ05_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005101980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY8565664.1"
                     /translation="MRQQQDEQGRFSICSRQAAVVRAPIDGIVANRSAHTGSWVEGGT
                     SLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKGEVFHGHIESLSPATGASF
                     SLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         5233 a         5050 c         4700 g         4946 t
ORIGIN      
        1 caggagagca tgaataatcc ggcactttct gaaacagaaa gtgaagctgc gctacaggcg
       61 atggtgtgtc tgcttcgccc agtacttcat cagcgggaat ctgtccaacc tcgccgtgaa
      121 cgtcagtttc aaaaagtggt tacgttgata gacgataata ttcgcgaaga gatattacgc
      181 ccggagtgga tagccggaga gacaggtatg tcagtacgta gtttgtaccg aatgtttgcc
      241 gataaaggtt tggtagtcgc gcaatatatt cgtaaccgtc gtctcgattt ttgtgcagat
      301 gcgattcgcc atgccgcaga tgatgaaaaa ctggcaggca tcggctttca ttggggattt
      361 tctgaccaga gtcatttctc aacggtattt aagcaacgct ttgggatgac gccaggcgag
      421 tatcgccgta aattccgcta actaattggc gttgcatgta ctgcaacgcc aattagttaa
      481 aacagatatt tgactatgca tttttcaggg cgaaaggcaa tttgacagga gtttttgaat
      541 attcaccttg tttcaggaaa atagatcaat cgctttctcc tgtagcgtta tcccgatatt
      601 gcggttttgc tattaaaact ttcgcaccca tttaccgttt atcccttgta catattcccc
      661 cggtttggcc cgagctacga gcttttgccc ggcgagtttc gcgatatcat ctacagacac
      721 attattttgc tttgcccgtt gttggtaact cgctttacgt tcggcattaa tttcggttac
      781 taatttctct gtttcagcat ccgttttcag cgcaaccaga taaccataaa atgtttcacc
      841 tacccgcccc tgagttctgg cttcatccag agtcaatgcc attacattgc tgcttaccag
      901 cccaacaaga aacgcacaaa gaagtaatgt tttcttcatc atcgcctcag aaaagatcgc
      961 tacgagtttc aagcagctct tcgacatctt tgtctgcctt gatgatgatc tcatgctcaa
     1021 ttttaacgtt catattgata gtgatcggtt ccttaggtgc agcgacttca atgcgaggcg
     1081 tacagccaac cagcataaaa gatgacgtca acgcagccag taaaattttc attgtttttc
     1141 ctcacactct tttccttgcg gacagtcatt tcccggcaga cgtgcgttct gctccaacca
     1201 tgcctggaga ttatcgccaa agcgtaaact gcgccacagc gtaaaaatat tctcttcatg
     1261 atggtaatta aggtttaccg taccgctttt accatcaacg cgactggtac ctgtaatgtt
     1321 ggcctgcatg gttaataaac cgagattatc taagttaatt tttgtcgatg accggctaat
     1381 ttccatatag cgcaaccagt taattgctga acccgcagtc atattgtctt tcaccaccgc
     1441 atctgcggta tctttatcca gccgcaaggt cattggcccg ctatttgcca gccaaccatc
     1501 tttcactatc catttttcgt tgtttagcca taacggcaat gcaccactaa atgccccgga
     1561 catggcgaat tgtttcggat tgacggcgct aaccagttcg ctggatgaga gattattcag
     1621 ccgcaacagc gccgggtcat gttgcggcat acgtaattgt ttcatcagca cgttaccgcc
     1681 taacacatcg acgctaacat cagtcaacag caagggttct tcttcagtcc acggataacg
     1741 cccttgcaaa tcagccgtaa tatttttcgc tgtcaccaga ttaatcactt cggcaattcg
     1801 caacgtaacg gggccgcgag tccccagatg ccaggctcca tcggcaaaac ggaaaggcag
     1861 gacaaaatcg acaccgttaa cctgattatc tggcatccag gcactaccgc ctttcaacac
     1921 gccgtgccct cccgcgcgga atccttgttc aggcgcagca gaaaatgcaa cctgagcata
     1981 cagttcacca tcgcgtaagt tcatcttcca gtcgggtggc accagcggct ggaatacagt
     2041 cagtgattgt tttggccacc aggcgttacc gcgcagacga ataccgtccc agcgaccatt
     2101 tacccgaacc gggccaatct caccaacatg taaatcgcct ttaaagagga aataggtcgg
     2161 atcgcgccca tcgacgctaa attttaaggt tgatggcggt aacacactgc cgccagtgaa
     2221 cagcgtttgc cctgcgtcca gtgacagcgc gccgctgaag gagggatgct gtgcgtcacg
     2281 tacccagacg acgggcttgt cgagaattaa tcgcggcttt tctaccgtca tcgtaccgta
     2341 ttgtaactgg tcgaaaccgg tggagagatc ggtcagcgta atcgtgctgt catgccactc
     2401 accttttcct gcgacatccc aggtggcatt catcggtgta aaactccctt ttccccagta
     2461 gcgccactgc cagcggccag cgtcagggag aaaatcattc gccagcccat ccatatgcag
     2521 cacgaaatcg cccagttcat tttcatgcgc ctgcaaaatg gcctgcaaac gtccgtcaac
     2581 accacgttgg gtgactttta cacccgctaa aggccagcgg atttcatcga tgtccagcga
     2641 atcgatgact cttccctttg aacgaagtaa cgcgcctggc tcaaaggtca gcgttgggtc
     2701 agacagactt ccacttagct gcgcaggtaa acgggcatat aaaatgaggt ccgcctgttt
     2761 cgcttcaccg gtcagctgca gaggcagttg actgttatcc atgcttaatt ttcctgggcc
     2821 aaaattaagc accgcgttgc ccttacccgc ttgcccctgg gtcagcacac tcagtcgtcc
     2881 gctgaccaga gcgttctcaa gccctgcctg ccagttgtca actttgacat ccagtcggcc
     2941 actcaaagga aaacctgcat acagccagct ccagcgacca tcgcttacgg tcaattgttg
     3001 acgagtaatt tgccacggca aatcgagcaa cggatcgccg ttatcccgtg ccagcacgat
     3061 caattgcccg ctattttcct gccagtccag ctcggcatcc accagtgacg gttcctgcgg
     3121 caagtttaac gtcgcagtag catgcccact tacaggaagt ccatccggca cgagcggcat
     3181 agtaaattcc cccaccagtt taaccggcgg ctgattttca aacgcgacga catccagttc
     3241 gctgactgta agttgttgcc ctttcagctg gccttgaaat ttaacttttt cgccctgata
     3301 acgcagttgc tggatatcag aggttaatgc gagagagagt tttccctgcc attcctgcca
     3361 gggagaaaaa atcagtttat cgatattgat ccaggtgtta ggcagcatgg actgccactg
     3421 cgcgagggtt tttggagcag ctggcgattg ctccgtctgc ggcaatttcg ccagacaagc
     3481 agaatcaagt tctaccgtgc cgacgttcaa taaccagcgg ctgggatgtg aaaggctggc
     3541 gttggtgata tgcgcaagct gacaatctcc caccagataa cggagatcgg gaatgattaa
     3601 acctttacgc gtaatgcgtg ggctttcatc taatgcaata cgtgtcccga gcggtagcca
     3661 gatgtccgcc agcgtgggaa cccacagccc gagcgtcatc agcagcgtca acggcacaag
     3721 aataatcagt aataacagcg cgagaacggc tttatattta cccagcatgg gtagttaata
     3781 tcctgatttc gcgaaaaatt aagcattcaa tacgggtatt gtggcatgtt taaccgttca
     3841 gttgaaggtt gcgcctacac taagcatagt tgttgatgaa tttttcaata tcgccatggc
     3901 tttcaattat atttgaaatt ttgtaaaata tttttagcag cttaaatgtg attcaacatc
     3961 actggagaaa gtcttatgaa actcgccgtt tatagcacaa aacagtacga caagaagtac
     4021 ctgcaacagg tgaacgagtc ctttggcttt gagctggaat tttttgactt tctgctgacg
     4081 gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat gtattttcgt aaacgatgac
     4141 ggcagccgcc cggtgctgga agagctgaaa aagcacggcg ttaaatatat cgccctgcgc
     4201 tgtgccggtt tcaataacgt cgaccttgat gcggcaaaag aactggggct gaaagtagtc
     4261 cgtgttccag cctatgatcc agaggccgtt gctgaacacg ccatcggtat gatgatgacg
     4321 ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg acgctaactt ctctctggaa
     4381 ggtctgaccg gctttactat gtatggcaaa acggcaggcg ttatcggtac cggtaaaatc
     4441 ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc gtctgctggc gttcgatccg
     4501 tatccaagtg cagcggtgct ggaactcggt gtggagtatg tcgatctgcc aaccctgttc
     4561 tctgaatcag acgttatctc tctgcactgc ccgctgacac cggaaaacta ccatctgttg
     4621 aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga tcgtcaatac cagtcgcggt
     4681 gcattgattg attctcaggc ggcaattgaa gcgctgaaaa atcagaaaat tggttcgttg
     4741 ggtatggacg tctatgagaa cgaacgcgat ctgttctttg aagataaatc caacgacgtg
     4801 atccaggatg acgtattccg tcgcctgtct gcctgccaca acgtgctgtt taccgggcac
     4861 caggcattcc tgacagcaga agctctgacc agtatttctc agactacgct gcaaaactta
     4921 agcaatctgg aaaaaggtga aacctgcccg aacgaactgg tttaatcttg ccgctcccct
     4981 gcaatccagg ggagctgatt cagataatcc ccaatgacct ttcattctct attcttaaaa
     5041 tagtcctgag tcagaaactg taattgagaa ccacaatgaa gaaagtagcc gcgtttgttg
     5101 cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa aattgctgtt acgccagaaa
     5161 tcagctttgg tgaaaaaatg atgatttccg gcagcatgtg taaccgcttt agcggtgaag
     5221 gcaggtgaac tgacagccaa agggctggca atgacccgta tgatgtgcgc taacccgcag
     5281 cttaatgaac tcgataacac cattagcgaa atgctgaaag aaggtgcaca agtggatctg
     5341 accgcgaacc agttaacgct ggcgaccgcg aaacagacat taacttataa gctggcggat
     5401 ttaatgaatt aatagctgcc acagctcccg gcggcaagtg actgttcgct acagcgtttg
     5461 ccgttgggta atgcacacat cccaatcgcc gtaccatcca gttgacgggc aacagaaagc
     5521 gaaccgccga tcattgcaca atttgcttct ccactactgg acatcgacgc ttttaaactt
     5581 ggcgctacgt gcgccgcagt cgcctgctga acaggctcac tactacacgc cgacaacaat
     5641 aaagcggcac accctaccca aaacgctgct cgcatctctt cttcctctga tcttcaagcc
     5701 aaacgacacc gccataaata ataggcagca cagagggcgg cgtcgagagc tgtcctgcgc
     5761 gttgccccgc atttttactt ttttatggct atttttttgc cctctgtttg atcaaaacat
     5821 tcattactct gatgtggggg acacaaaagc gaaaatgcag aagaaagcca tttgctaaaa
     5881 ttgaaggatt actactgggc gcgcagcaat ttcgtgcgcc cctcatttgc gcaatgtaag
     5941 ggtgtcatat gattactatt gacggtaatg gcgcggttgc ttcggtggcg tttcgcacca
     6001 gtgaagttat cgccatctac cctattaccc ccagttccac gatggcagaa caggctgatg
     6061 cctgggccgg aaacggcttg aagaacgttt ggggagacac accacgcgtg gttgaaatgc
     6121 agtcggaagc gggtgctatc gctaccgtgc atggcgcttt gcagacgggt gccctttcaa
     6181 catcgtttac gtcatcgcag ggtttgctgc tgatgatccc gacgctgtac aaactggcag
     6241 gcgaactgac gccatttgtc ctgcatgtag cggcgcgtac cgttgccaca catgcacttt
     6301 ctatttttgg cgatcattcc gacgttatgg cggtgcgcca gacgggttgc gcgatgttgt
     6361 gtgcggctaa cgtccaggaa gcgcaagact ttgctctcat ttcgcacatc gcaacgctga
     6421 agagccgcgt gccatttatt catttctttg atggttttcg cacgtcccac gaaatcaata
     6481 aaattgtccc gctggccgat gacactattc ttgatctcat gccgcaggcc gaaattgatg
     6541 ctcatcgcgc ccgggcgctc aacccggaac atccggtgat ccgcggtacg tccgccaatc
     6601 ctgacactta tttccagtct cgcgaagcca ccaacccctg gtacaacgcg gtctatgacc
     6661 atgttgaaca ggcgatgaat gatttcgctg ccgcgacagg tcgtcagtat cagccgtttg
     6721 agtattacgg gcatccgcaa gcggaacggg tgattatcct gatgggctct gccattggca
     6781 cctgtgaaga agtggttgat gaattgctaa cccgtggcga aaaagttggc gtgctgaaag
     6841 ttcgtctgta ccgtcccttc tccgccaaac acttgctgca agctctgccg ggatccgtac
     6901 gcagcgtggc ggtactggac agaaccaaag aacccggtgc ccaggcagaa ccgctctatc
     6961 tggatgtgat gaccgcactg gcaggagcct ttaataatgg tgagcgcgaa accctgcccc
     7021 gtgtcattgg tgggcgctat ggtctttcat ccaaagaatt tggcccggac tgtgtactgg
     7081 cggtatttgc cgagctcaac gcggctaaac ctaaagcacg ctttacggtt ggtatttacg
     7141 atgatgtgac caatctgtca ctgccgttgc cggaaaacac tctgccaaac tcggcgaaac
     7201 ttgaagcctt gttttatggc cttggtagtg atggcagcgt ttccgcaacc aaaaacaata
     7261 tcaagattat cggtaattcc acgccgtggt acgcacaggg ctattttgtt tacgactcca
     7321 aaaaagcggg cggcctgacg gtttctcacc ttcgcgtgag cgaacagccg attcgttccg
     7381 cttatctcat ttcccaggct gattttgttg gctgccacca gttgcagttt atcgataaat
     7441 atcagatggc tgagcgttta aaacctagcg gcattttcct gcttaatacg ccgtacagcg
     7501 cagatgaagt gtggtcgcgc ttgccgcagg aagttcaggc cgtgttaaac cagaaaaaag
     7561 cgcgcttcta tgtgattaac gcggcgaaaa tcgcccgcga atgtggcctg gcggcccgta
     7621 ttaataccgt catgcagatg gcttttttcc atctgacgca aattctgcct ggcgatagcg
     7681 ccctcgcaga attgcagggt gcgattgcca aaagttacag tagcaaaggt caggatctgg
     7741 tggagcgcaa ctggcaggct ctggcgctgg cgcgtgaatc cgtagaagaa gttccgttgc
     7801 aaccggtaaa tccgcacagc gccaatcgac cgccagtggt ttccgatgcc gcccctgatt
     7861 tcgtgaaaac cgtaaccgcg gcgatgctcg ccgggcttgg tgatgccctc cccgtttcgg
     7921 cgctgccgcc agacggcacc tggccgatgg gcaccactcg ctgggaaaaa cgcaatatcg
     7981 ccgaagagat ccccatctgg aaagaggaac tctgtaccca atgtaaccac tgcgtcgccg
     8041 cttgcccaca ctcagctatt cgcgcaaaag tggtaccgcc tgaagcgatg gaaaacgccc
     8101 ctgccagcct gcattcgcta gatgtgaaat cgcgtgatat gcgcgggcag aaatatgtct
     8161 tgcaggtggc accggaagat tgcaccggtt gtaacctgtg cgtcgaagtt tgcccggcga
     8221 aagaccgtca gaatccagag attaaagcca tcaatatgat gtctcgccag gaacatgtcg
     8281 aagaagagaa aatcaattac gatttcttcc tcaacctgcc agaaatcgac cgtagcaaac
     8341 tggaacgtat tgatattcgt acatcgcagc tgattacacc gctgtttgaa tattcaggtg
     8401 cttgccccgg ttgtggcgag acgccgtata ttaaattgct gactcagctc tatggtgacc
     8461 ggatgttgat cgctaacgcc accggctgtt cttcaattta tggcggtaac ctgccctcta
     8521 caccgtatac caccgatgcc aacggtcgtg ggccggcatg ggcgaactct ctatttgaag
     8581 ataatgccga atttggcctt ggtttccgcc tgacggtcga tcaacaccgt gtccgcgtgc
     8641 tgcgtctgct ggatcaattt gccgataaaa tcccggcgga attactgacg gcgttgaaat
     8701 cagacgctac gccagaggtt cgtcgagaac aggtcgcagc tttacgccag caactcaacg
     8761 atgttgccga agcacatgaa ctgctgcgtg acgcagatgc actggtggaa aaatcaatct
     8821 ggctgattgg tggcgacggc tgggcttacg atatcggctt tggcggtctg gatcatgtat
     8881 tgagtttgac ggaaaacgtc aacattctgg tgctggatac gcaatgctat tccaacaccg
     8941 gtggtcaggc gtcgaaagcg acaccgctgg gtgcagtaac taagttcggc gagcacggaa
     9001 aacgtaaagc gcgtaaagat cttggcgtca gtatgatgat gtacggtcat gtttatgtgg
     9061 cgcagatttc tctcggcgcg cagctgaacc agacggtgaa agcgattcag gaagcggaag
     9121 cgtatccggg accatcgctg atcattgctt atagcccgtg tgaagagcat ggttacgatc
     9181 tggctctcag ccacgaccag atgcgccaac tcactgctac cggcttctgg ccgctatatc
     9241 gctttgatcc gcgtcgtgcc gatgaaggca aactgccgct ggcgctggat tcacgcccgc
     9301 cgtcagaagc actggaagaa acgttacttc acgagcagcg tttccgtcgt ctgaattcgc
     9361 agcagccaga agtggcagaa cagttatgga aagatgctgc agctgatttg caaaaacgct
     9421 atgacttcct agcacaaatg gccggaaaag cggaaaaaag caacaccgat taaatgctct
     9481 ggataaggat tatccaattc taaaaaaaaa gcccggacga ctgttcgggc ttgtcttttt
     9541 atatgttgga aaatcagtgg caatgcaatg gcccaacaga aaataaaatc tgtgacaata
     9601 aaggcatata acccgcgcag aataacgtat agaaaattaa aagtataatt tttattttgt
     9661 atgtattatc ttcgatacat tcaccagact tattatcatt tcagaggaat tatttgatta
     9721 aggttttact taaggcgtaa caaatgatat ttatcggcaa actgaacttc tcctttagga
     9781 tgttttcact ccccgaaatg gggataaaag gcaaataaaa taactaaagg atttattcaa
     9841 tgaaaagaaa agcactggca cttttaatcc ctgccctgct cgccgcaggt gctgcacatg
     9901 cagccgaagt ttataataaa gacggcaaca aattagatct gtatggcaaa gttgatggcc
     9961 tgcattattt ttctgataat tcagcgaaag atggcgacca gagctatgcg cgtctgggtt
    10021 ttaaaggcga aacccaaatt aacgatcaac ttactggcta cggtcaatgg gaatacaata
    10081 ttcaggcaaa caacactgaa tcttcaaaaa accagtcatg gacccgtctg gcatttgccg
    10141 gactgaaatt tgcagattac ggttctttcg attacggacg taattatggc gtaatgtacg
    10201 acatcgaagg ctggaccgat atgctgccta aatttggcgg tgactcttat accaatgcag
    10261 acaactttat gactggtcga gccaatggcg tcgcgactta tcgtaatact gatttcttcg
    10321 gtctggtaaa tggtctgaac ttcgcggtgc agtatcaagg taacaacgaa ggagccagta
    10381 atggtcagga aggcaccaat aacgaacgcg atgttcgtca tgaaaacggt gacggctggg
    10441 gtctttccac aacatatgat ttaggcatgg gctttagcgc tggtgcggca tacacctctt
    10501 ctgaccgcac caatgaccag gttaaccata ctgcggcggg tggtgataaa gcagacgcgt
    10561 ggactgctgg gctaaaatac gatgctaaca atatttacct agcaaccatg tattcagaaa
    10621 cgcgtaatat gaccccgttt ggcgacagcg attatgcagt tgcgaacaaa acccagaact
    10681 ttgaagttac tagtaccagt ttgattttgg tctgcgtccg gcagtctctt tcctgatgtc
    10741 taaaggccgt gaccttcacg ctgctggtgg tgcagacaac ccggcaggtg ttgatgataa
    10801 agatctggtt aaatacgccg atgttggcgc gacttactat ttcaataaaa acatgtccac
    10861 ctatgttgac tataaaatca acctgttgga tgaagatgac agtttctacg ctgccaacgg
    10921 catctctacc gatgatattg tcgctttagg tctggtttat cagttctaaa tcctcctgcc
    10981 cgctgttatg gcgggctttt tctgcttatt cttcctcctt tgatctaaat taaaaatgtg
    11041 aactccgtca ttacacaaaa agtgtcatct ggcgttacac tttatgcgga tactaaaaca
    11101 ggaggtttta tgaacagaac gattcttgtc cctatcgata tttcagattc agaattaact
    11161 caacgcgtga ttagccacgt tgaggcagag gcaaagattg atgatgcaga ggttcatttc
    11221 ctgacggtaa taccttcact gccctactat gcctctctgg gtttagcgta ttccgcagaa
    11281 ttaccggcaa tggatgacct gaaagcggaa gccaaatcgc aactggaaga gatcattaaa
    11341 aaatttaaac tgccaaccga cagagtgcat gtccatgttg aggaaggctc gcccaaagac
    11401 cgcattctgg aattggcgaa gaagatcccc gctcatatga tcatcattgc ttcccatcga
    11461 ccggatatca ccacttatct gctcggttcc aacgccgcag ctgtagtgcg tcacgcagag
    11521 tgctccgtgc tggttgtgcg ctgacactaa cgcccgcaca ttgctgcggg ctttttgatt
    11581 catttcgcaa atgtgctgac attttcccct ctaatccgta ccatacacgc cacagttttt
    11641 atatcagatt tcttatgctg agcgttccgg catgacgcat actcttctga tgccatataa
    11701 cgaattgagt cgcttttaaa tgtcgcaaaa tcaagaaatt agtaagaaag aacaatacaa
    11761 cctgaacaaa ttacaaaaac gtctgcgtcg taacgtgggc gaagccattg ctgacttcaa
    11821 tatgattgaa gaaggcgatc gcatcatggt ttgcctctcc gggggtaaag acagctatac
    11881 catgctggag attctgcgca atttgcagca aagcgcgcca atcaattttt cgctggtggc
    11941 tgttaacctc gatcaaaagc aaccgggctt cccggaacac gttctgcccg agtatcttga
    12001 aaagctgggc gttgagtaca agattgttga agagaacact tacggtatcg tgaaagagaa
    12061 gattccagag ggcaaaacca cttgctcact gtgttctcgg ctacgtcgcg gtatccttta
    12121 tcgcaccgca acggaactgg ggacgacgaa gatcgcgttg ggtcaccatc gtgacgatat
    12181 cctgcaaacg ttgttcttaa atatgttcta cggcggcaag atgaaaggta tgcctccgaa
    12241 actgatgagc gatgatggca aacatatcgt tattcgtcca ctggcctact gccgcgagaa
    12301 agatattcag cgatttgccg atgcaaaagc gttcccgatt attccgtgca acctgtgcgg
    12361 ttcacagcct aacctgcaac gtcaggtgat tgctgacatg ttgcgtgact gggataaacg
    12421 ttatccaggg cgtatcgaga cgatgttcag cgcgatgcag aatgtggtgc cgtcgcatct
    12481 gtgcgatacc aacctgttcg atttcaaagg cattacccac ggttctgaag tggttaacgg
    12541 gggtgatctg gcgtttgatc gcgaagagat cccactacaa ccggcgggct ggcagccaga
    12601 agaagatgaa aatcagttgg atgagttacg gctgaatgtg gttgaagtga aataaccagg
    12661 atagcgcccg atgcgcaagc gtatcgggct actcttatgg aggccggata agacgcggcc
    12721 agcagcgtcg catccggcaa tcccgaataa gatgtttact cttgcacccg gcaattcaac
    12781 atttcattat tttaataacc gcacccggca cgtttttcct ttaatcttcc cgccctgtaa
    12841 ctgtttccat gctttatgag caacagcctg acggaccgcg acatagacat gcgccggatg
    12901 cacggcgatt ttgccaatat ctgcgccatc aagcccgata tctcctgtca gtgcacctaa
    12961 tacatcaccc gggcgcattt tggctttttt cccgccatcg atacacaacg ttgccatttc
    13021 tgcttccagc gtcacaatgg aactattagc tggcggcgtt tgccagttaa gttttatctg
    13081 caacatgtca gaaatgatat tggcccgctg tgcttcttcc ggagcacaga aactgatcgc
    13141 cagaccgcta tttcctgcac gagctgtacg accgatgcga tgtacatgaa cttcagggtc
    13201 ccacgccagc tcaaagttca ccaccagctc aagcgattta atatccagac cacgcgcagc
    13261 aacatcagtc gcaaccagta cacgggcgct accgttagca aaacgtacca gggtctgatc
    13321 gcgttgctcc agatcgccgt gtaacgacaa tgcactttgc tctacttcat tcagcgcgtc
    13381 gcagacagcc tggcaatctt ttttggtatt gcaaaacacc acacaagagg atggctgatg
    13441 caagcttaat aaccgttgca acagaggaat tttgccttta ctggatgtct cataaaattg
    13501 ttgttcaatg ggtggcaaag catctgttga gtcaatttca atcgccaaag gatcgcgttg
    13561 cactcgtccg ctgattgcgg cgatggcttc cggccaggtt gccgaaaaca gaagcgtctg
    13621 tcgagatgca ggcgcaaaac ggatgacatc atcaatggca tcgctaaatc ccatatccag
    13681 catgcggtcg gcctcatcca tcaccaaagt attcagcgca tccagtgata ccgtgccttt
    13741 ttgcaagtga tccagcaaac gacccggcgt tgccacgata atatgcggcg catgttgcaa
    13801 cgaatcacgc tgcataccga acggttgacc accgcacaac gtcaaaattt tggtatttgg
    13861 cagaaaacgc gccagccgac gcaattcacc tgccacctga tccgccagtt cacgcgtagg
    13921 acacagcact aaagcctggg tttgaaatag cgacgcatca atttgctgta acaagccgag
    13981 gccaaaagcc gccgttttgc cgctgccggt tttcgcctgc acgcgaacat cttttccggc
    14041 aaggatcgcc ggaagcgcgg cggcctgcac cggcgtcatg gttaaataac ccaactcatt
    14101 aaggttcgtg agttgggcgg gaggcaaaac attcagggta gaaaaagcgg tcacaatcta
    14161 ttctcgtggt catcgacgca aagttagcag gcgcgtatcc tcgcagatct acgctcacga
    14221 tgcgacaatt taatcggttc ttcatcgggt ggtgggtcag gcatgggttg cgggcgaggg
    14281 atcggatcgg gcactggaac aggatcgcca ggaatcggtt cagggacagg aatttgcaaa
    14341 taaataagtg tcgtcatatt tccctccggt cattgggtgg actcttaaag ggtagacgct
    14401 gataaataac aggcaaaaaa aagccgactc atcaaagtcg gcgtcgtacg aatcaattgt
    14461 gctatgcagt aattcaaaaa aggaagtaag acaatatgga gcgcaacgcc catcgcttga
    14521 cgttgcattc acctgcaagg gagatattgc cctgaatggg tagagagttt attgacttcg
    14581 ctcaaacttt gcggcgtttt tgtatacaga cagccggaaa aattgctttt gttacaacca
    14641 tttactacgg tgcaaccata aagcaacacc accaataaga acaactaaca gaatacaaaa
    14701 aattgaaaat ccgaattgcc acccgccgcc agggatccca ccaaggttga cgccaaataa
    14761 cccggtcaga aaggtactgg gtaaaaagac cattgccatc aacgacattg tataggtacg
    14821 acgagctaaa ttttcctgca tcacctgagc gatttcatcc gccatcacgc cagtccgtgc
    14881 tatacaggcg tcgatttcgt caaggccgcg cccaaggcga tcggcaatat cctgcatccg
    14941 acggcgttgg tcatcgctca tccacggcaa acgttcactg gcaagacgag cataaacatc
    15001 acgttgcggt gccatatagc gacgcatcac aattaattgt ttgcgcagca gagccaggaa
    15061 tccacgcggt ggaatttgct gatcaaggag attatcttca aggtcgataa ttttatcgtg
    15121 cagctgctcg ataaattcac tggaatgatc ggtcaacgca tcgcacacat ccaccagcca
    15181 tcccccgcaa tcggtcggac ccgtgccctc ttccagatcg ctcaccacat cgtccagcgc
    15241 cagcactttg cgttgtcggg tcgaaataat taaccgcccg tccatatata cacgcatggc
    15301 gaccagttga tcggggcgtt catcggtgct gccgtttata cagcgcaatg taatcagcgt
    15361 gccttcaccg aaacggctga ctcggggccg tgtgctctcg cccgccagcg catcacgtac
    15421 gttattggga agcagcggtg ttgtcgccag ccattgggcg ctatcatggt gtacataatt
    15481 aaggtggagc cagcagggat gcgcttcatc aatcacatct gtattttcca gcggtttaac
    15541 gccgcctcta ccatccagca tccaggcaaa tactgcatcc gggacattaa cgtccgatcc
    15601 cttaatcgct tccacagtgc ctccatcatc aacgcattat tttgtagtct agccttctgg
    15661 ccctgttacg caacatctca tcaccccatt accctgaaat gattaataaa attctgtcta
    15721 aattgaatac aaaaagcaaa atgcttttcc gtatacaaac cgtgtgaagt gttaaatagc
    15781 gtctatcatt atcagaatta tctgatcata tgacgtggct tttttgcgat cggatagcaa
    15841 caaaaattga taaaaataac gggatctcaa tgattacgca caacttcaat accctggact
    15901 tactcaccag tcctgtctgg attgtttcgc cctttgagga acagttaatt tatgccaata
    15961 gcgcggcgcg actgttgatg caagacctca cgtttagtca gctacgaacc ggaccctatt
    16021 ccgtctcctc acaaaaagaa ctgccgaaat acctctccga tctgcaaaac caacacgata
    16081 ttatcgaaat cctcactgtt cagcgtaaag aagaggaaac agcattaagc tgtcggcttg
    16141 ttttgcgaga gctgacagaa acagaaccgg tgattatttt cgaaggtatc gaagcgccgg
    16201 caacgctggg tttaaaagcc agtcgctcgg caaattatca gcgcaaaaaa caaggttttt
    16261 atgcgcgctt ttttctgact aactctgcac caatgttgtt gattgacccg tcacgagatg
    16321 gacaaatcgt cgatgctaac ctcgccgcgc tcaatttcta tggttataac catgaaacga
    16381 tgtgccagaa acatacctag gaaataaata tgcttgggcg tcgcgtcatg cctatcatgc
    16441 atgaaatttc gcatttaccc ggcggtcata accctttgaa ttttattcat aaactggcgg
    16501 atggttctac tcgtcatgtg cagacctatg ccggaccgat tgaaatttat ggcgacaagc
    16561 tcatgttatg tattgtgcat gatattactg agcaaaaacg gctggaggag cagctggaac
    16621 atgctgctca ccatgacgcg atgaccggat tactgaatcg gcgacagttt tatcacatta
    16681 cggaaccagg ccaaatgcag catctcgcca tcgctcagga ttacagcttg ttgctcatcg
    16741 acaccgatcg ttttaaacac attaacgatc tctatgggca ttctaaaggt gatgaggtgt
    16801 tatgcgccct cgcccgcccc ctcgaaagtt gcgctcgcaa aggcgatttg gtgtttcgtt
    16861 ggggaggcga agagtttgtc ttattgctac caagaacccc actggatacc gcgctttcgc
    16921 tggctgaaac tatccgcgta agcgtggcaa aagtgagtat ttcgggctta ccacgcttta
    16981 ccgtcagcat tggtgtggcg catcacgaag gaaatgaaag catcgatgaa ctgtttaaac
    17041 gcgttgatga tgctttgtat cgggcgaaaa atgatggacg caaccgcgtg ctggcggcat
    17101 aagccgcgga tgcgtctcga gatcaacgac tgcgcttagc gtggcgctcc cagttttctt
    17161 gcttcgcctg cgccgtttta cgtagtgcga cgtaacacgc cccgctgccg ccatgatgcg
    17221 gtagcgcggt gcaatatgcc tgaacatcat caaattcggt cagccagcgc gccacatagc
    17281 tgcggacaat attggcatgt gatttatctt cccgcccttt accatgaata atcagcacat
    17341 tacgcaaacc atccgccagg gcttgttgaa tgaaactgaa caccattttg cggcactctt
    17401 ccaccggctg gcgcaaaaga ttcaggctcg cctgttgcgg atatttacca ctgcgcagct
    17461 tatccagcac cccatgctgc aacccttcct gccgaaactc cagcggctga cttagcggga
    17521 tgatgtcgag aaatccggta gtgaggaaat tatcaagctg cagcgtgtcg atacgctgcg
    17581 gcgcacgttg gttacgcgtt ggatgccagt ggacatctgt agcacgtttc agcggctgga
    17641 catcttccat ggcgtcaaga aacagcgatt tgtcgtcaag gttcatgtaa cctcctatgc
    17701 accttcacac gtcgtgattc agacgggtta attgttcata cttactgcat cgttcccttc
    17761 acagattgaa acaatactct acttcatacc tgtgagtatc tgcgcaggcg gggcatttta
    17821 tactagcctg ttagcagtat cgaacagttt agcccgagtt taattcagca caggctccga
    17881 tcaggatgat ttattcatag caggaaccaa caaaaatgcg ataaataaca gaccactgat
    17941 tagcagaaag gtaccgaaaa tgccatagtt tgcgcctccc tcagcatcaa ttgactcaat
    18001 aatttggttg aagccagtat ttccgatgtc tggtcacttc tactgcgatt aaaaaataaa
    18061 gcacttctgg aaatgaagtc attcaccgta tggggaacag aaaccatttt ttcacccatt
    18121 ctcgaaaaat gaaaattagt tcgattattg agaatagagc cacacagggc aataccaatc
    18181 gcgcccccaa gattacgtgt taaattaaat acaccagagg ctaacttcag ccgttctttt
    18241 ggaattcccc ccagcgttaa tgttacgatg ggggccatcg caaactgctg caaaatccct
    18301 ctaattgcct gtggaaacag taattcctgc catccccatt cgtgggttat aggggtaaaa
    18361 agatacattg aaaacacaaa tccgcccagc cccgccatca acagccatcg cagatttatt
    18421 tttttactta accagaagta aaagggaaca gaaaataact ggaaaatacc tgttgtgcaa
    18481 acggcaaaac ctatctcttc ggcatttaat ccgcgaatct gccccaaaaa cacaggtatt
    18541 aaatatacag tagagaatat cccaacccct ccagaaaaag aaaaaaaaca tcccagagtg
    18601 aaatttttat ccttaaaagc atgaagatcc attatcggat tggatattgt aagtgtacgc
    18661 gcagcaaata aaataaaact caccaatgcc agaactgatg tcagcaaaat agtattatca
    18721 tccagccatc cccagcgcgc tccttcttca agggtatatt ccagacatcc cagagtcatg
    18781 gccaacagga taatactcgg ataatcagca actttcagta aagagagatc aggtttatca
    18841 aaattaacca gaaaaggaat actcaaaact aaatatatgc caggtaatat attgatataa
    18901 aatagccatc gccagtcgag gttatcagtg agccagcccc ccagagttgg ccctagcgtc
    18961 ggtgacagtg aggccagcgc actgactacc gcagcagcca accccaactc tttaccctgg
    19021 taataaataa acgccgtcgt gaaaactaaa gggatcatgg atgcgccagc agctccctgt
    19081 aaagcacgaa acaaaatcat gatctgaata ttccatgcaa gtccacacgc gatactcatt
    19141 aaagtaaata ttcccgcgga tagagtaaaa agccaacgag tggaaaatac ccgtgacagc
    19201 cacccagaga gcggaataac aataatctca gcaattaaat agctggtctg aagccaggcc
    19261 atttcgtctt tacctgcgga cagaccacca cctatttcat ttagggatga cgatacaatt
    19321 tgaatatcaa gctgagcaat aaataatccc atacacatac tggcgaaagc aatatgttta
    19381 cgcatagaca tcatctctca tcaacactga cggtgacgga cagtcccggg cgaagaagtt
    19441 gctttaattc ctttgcgtcg tcaaatgcaa tccgtaccgg gacacgctga acaattttgg
    19501 tgaaattgcc agtggcattt tctatcggaa tcaagctgaa actggctcct gtcgctgggg
    19561 aaaggctttc aatgtgaccg tgaaacactt cccctttgag aatatcagca cggatctcag
    19621 ccttcattcc tggcttcatt cccgctattt ggttttcttt ataatttgca tcaacccaca
    19681 actcactgac tggtactagt gaaactaacg atgtgccgcc ttccacccaa ctgcctgtat
    19741 gggcgctgcg gtttgcgaca atgccatcaa tcggcgcacg aaccacggca gcctgacggc
    19801 tgcaaattga aaaccgcccc tgttcgtcct gttgttgccg cacgctccgc agaatatgta
    19861 tgctctgagg acctgtttta ttaaggactc atcctcttat gttgacccgc aaaagtatag
    19921 ataccgttc
//
LOCUS       DADMEQ010000068        19914 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.068, whole genome shotgun sequence.
ACCESSION   DADMEQ010000068 DADMEQ010000000
VERSION     DADMEQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.068"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..744)
                     /locus_tag="JNZ05_002464"
     CDS             complement(<1..744)
                     /locus_tag="JNZ05_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8565665.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADSDTDSY
                     MWFETGDNGNEYFKWRHHLTGGRVKDLMNLKWDALYVLVKALFSSEVKISTVNALRIF
                     NSSFGAIFRRSEECLHIIPTRENEGENGNIGPLRPFTLNLRTGRISMGHGLVVTGDIF
                     TNRFLINSSTGMWIHMRDQNVIMGRNAVSNDGAQALLRQDHTDRKFVIGGLGNRQFGI
                     YMINNSRTANGTDGQAYMDNNGNWLCGSQV"
     gene            838..1035
                     /locus_tag="JNZ05_002465"
     CDS             838..1035
                     /locus_tag="JNZ05_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY8565666.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTRTDRPG
                     LKRALKRLQKGTHIENDDRIN"
     gene            1129..1317
                     /locus_tag="JNZ05_002466"
     CDS             1129..1317
                     /locus_tag="JNZ05_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY8565667.1"
                     /translation="MKSIRCKNCNKLLFKADSFDHIEIRCPRCKRHIIMLNACEHPTE
                     KHCGKREEITHSDETVRY"
     gene            1238..1963
                     /locus_tag="JNZ05_002467"
     CDS             1238..1963
                     /locus_tag="JNZ05_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HAY8565668.1"
                     /translation="MPASIPRRNIVGKEKKSRILTKPCVIEYEGQIVGYGSKELRVET
                     ISCWLARTIIQTKHYSRRFVNNSYLHLGVFSGRDLVGVLQWGYALNPNSGRRVVLETD
                     NRGYMELNRMWLHDDMPRNSESRAISYALKVIRLLYPSVEWVQSFADERCGLAGVVYQ
                     ASNFDFIGSHESTFYELDGEWYHEITMNAIKRGGQRGEYLRANKERAVVHKFNQYRYI
                     RFLNKRARKRLNTKLFKVQPYPK"
     gene            complement(2075..2422)
                     /locus_tag="JNZ05_002468"
                     /pseudo
     CDS             complement(2075..2422)
                     /locus_tag="JNZ05_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2391..4048)
                     /gene="dpiB"
                     /locus_tag="JNZ05_002469"
                     /pseudo
     CDS             complement(2391..4048)
                     /gene="dpiB"
                     /locus_tag="JNZ05_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            4429..5454
                     /gene="citC"
                     /locus_tag="JNZ05_002470"
     CDS             4429..5454
                     /gene="citC"
                     /locus_tag="JNZ05_002470"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAY8565669.1"
                     /translation="MFVNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKLRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWFHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSR"
     gene            5502..5798
                     /gene="citD"
                     /locus_tag="JNZ05_002471"
     CDS             5502..5798
                     /gene="citD"
                     /locus_tag="JNZ05_002471"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAY8565670.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5795..6703
                     /gene="citE"
                     /locus_tag="JNZ05_002472"
     CDS             5795..6703
                     /gene="citE"
                     /locus_tag="JNZ05_002472"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAY8565671.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6714..8246
                     /gene="citF"
                     /locus_tag="JNZ05_002473"
     CDS             6714..8246
                     /gene="citF"
                     /locus_tag="JNZ05_002473"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAY8565672.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            8250..8801
                     /gene="citX"
                     /locus_tag="JNZ05_002474"
     CDS             8250..8801
                     /gene="citX"
                     /locus_tag="JNZ05_002474"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAY8565673.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8776..9654
                     /gene="citG"
                     /locus_tag="JNZ05_002475"
     CDS             8776..9654
                     /gene="citG"
                     /locus_tag="JNZ05_002475"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAY8565674.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            9705..11168
                     /gene="citT"
                     /locus_tag="JNZ05_002476"
     CDS             9705..11168
                     /gene="citT"
                     /locus_tag="JNZ05_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HAY8565675.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMDKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNML
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            11282..12088
                     /gene="rna"
                     /locus_tag="JNZ05_002477"
     CDS             11282..12088
                     /gene="rna"
                     /locus_tag="JNZ05_002477"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HAY8565676.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            12317..12727
                     /gene="rnk"
                     /locus_tag="JNZ05_002478"
     CDS             12317..12727
                     /gene="rnk"
                     /locus_tag="JNZ05_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HAY8565677.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLRDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(12958..14195)
                     /locus_tag="JNZ05_002479"
                     /pseudo
     CDS             complement(12958..14195)
                     /locus_tag="JNZ05_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            14416..14844
                     /gene="uspG"
                     /locus_tag="JNZ05_002480"
     CDS             14416..14844
                     /gene="uspG"
                     /locus_tag="JNZ05_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HAY8565678.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(14965..16530)
                     /gene="ahpF"
                     /locus_tag="JNZ05_002481"
     CDS             complement(14965..16530)
                     /gene="ahpF"
                     /locus_tag="JNZ05_002481"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAY8565679.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(16775..17338)
                     /gene="ahpC"
                     /locus_tag="JNZ05_002482"
     CDS             complement(16775..17338)
                     /gene="ahpC"
                     /locus_tag="JNZ05_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HAY8565680.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            17710..18456
                     /gene="dsbG"
                     /locus_tag="JNZ05_002483"
     CDS             17710..18456
                     /gene="dsbG"
                     /locus_tag="JNZ05_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HAY8565681.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            18666..19568
                     /locus_tag="JNZ05_002484"
     CDS             18666..19568
                     /locus_tag="JNZ05_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8565682.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            19715..19914
                     /locus_tag="JNZ05_002485"
                     /pseudo
     CDS             19715..19914
                     /locus_tag="JNZ05_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         5019 a         5127 c         4754 g         5014 t
ORIGIN      
        1 aacttgcgaa ccgcaaagcc agttcccgtt attatccatg tacgcctgac catcggtgcc
       61 attggctgtc cttgagttat taatcatgta gatgccaaat tgcctattcc ccagcccacc
      121 aattacgaat ttacggtcgg tatggtcctg gcgaagcaat gcctgcgcac catcgttgga
      181 taccgcatta cggcccataa taacgttctg gtcacgcata tgaatccaca tgcctgtact
      241 gctgttaatt aaaaaacggt ttgtaaatat atctcctgta acaaccagac catgccccat
      301 gcttatccgg ccagttctga gattaagcgt aaaggggcgt agtggcccta tattaccatt
      361 ttccccttca ttctctcgtg tagggatgat atgcaggcat tcttcagaac gacgaaaaat
      421 ggcaccaaaa gatgaattaa atattctcag tgcattgacc gtcgatattt ttacttcact
      481 gctgaaaagg gctttaacaa gaacatacag agcatcccat ttaagattca tcaggtcttt
      541 aacccggccg ccagtgaggt ggtgtctcca tttgaaatac tcattgccgt tgtcgcctgt
      601 ttcaaaccac atgtatgaat cagtatcgct gtcggcatca tttttaaatc caatctttgc
      661 ccagtcagta ttccgaatcc aggcaaggat tgagtcgttt tcaaaagtaa gtccaccgga
      721 caacgtatcg ccattttttt gcacggcgtt accagccttg tttaccgttt cctgtaaacc
      781 gaggggccgt ttctggcctg catggcatga tttccgcttt taaacgggga attcagcgtg
      841 ctgattggct atgtaagggt atcaacaaat gaccagaata cagacctgca acgaaacgct
      901 cttgtttgtg caggatgtga acaaatattt gaagataaat taagcggaac aaggacagac
      961 cgaccgggat taaaacgtgc tttaaagcgc cttcaaaaag gaacgcatat agaaaacgac
     1021 gatcgaatca attaaatcga tctatattac cgatcgatta tgtcccgata accacacccg
     1081 actgatgaga ttttttaaga taacggcgaa ttgatgcaag gaggtgaaat gaaatcaatt
     1141 cgctgtaaga actgcaacaa actgttattt aaagcggatt cctttgatca cattgaaatc
     1201 aggtgtccgc gttgcaaacg tcacatcata atgctgaatg cctgcgagca tcccacggag
     1261 aaacattgtg ggaaaagaga agaaatcacg cattctgacg aaaccgtgcg ttattgagta
     1321 tgaaggccag attgttggct atggttcaaa ggagctgcgc gttgaaacca tatcctgctg
     1381 gctggcccgc acaatcattc agacaaagca ctattcccgc cgttttgtga ataactctta
     1441 ccttcacctg ggagtattca gcgggcgtga tctggttggc gttctccagt ggggatatgc
     1501 ccttaacccc aactcaggtc gtcgtgtcgt gcttgaaacg gataaccggg gctatatgga
     1561 gttgaatcgc atgtggctac acgacgacat gccccgcaac tctgaatcac gggccatcag
     1621 ctacgcgctg aaagttatca gattactgta tccgtcagtg gagtgggttc agtcctttgc
     1681 tgacgaacgc tgcggactcg caggcgttgt gtatcaggcg tcgaattttg attttattgg
     1741 cagccatgaa agcacgttct acgagctgga tggtgaatgg tatcacgaga taacgatgaa
     1801 cgcgattaag cgcggagggc aacgaggcga gtatttgcga gctaataagg agcgtgccgt
     1861 ggtgcacaaa tttaatcagt atcgctacat cagattcctg aacaagcgag caaggaagcg
     1921 gctaaatacc aaactattca aggttcagcc ataccctaag tgatccccat gtaatgaata
     1981 aaaagcggta attaatactg ctgtttcatt tgaagcgcga aagctaaagt tttcgcattt
     2041 atcgtgaaac gctttcgcgt ttttcgtgcg ccgcttcacc cagccgttca taggcaattg
     2101 gcttaatgag ataatcaaat acaccacaac gtacggcttc agacaccgtt tccatatcac
     2161 tggctgcagt ggtaaacacc acgtcgccgg gataatgcgc ctgcaccagt tcatgcagta
     2221 aattaatccc tctaccgtca ggaagatagt tatcgagcaa gattagcccc ggcttaaaac
     2281 gctcgatcat cattcgggcc tgcgccaggt ttcccgccag taatatctga ctgaatccgg
     2341 gaatgtgacg aatatattcc gcatgcatct ctgccagcgg cgtttcgtcc tcaacgatca
     2401 atagggttaa tggagctgtc attaggtttc actttcggaa tatagattga aaataaggta
     2461 ccgcagggat cattatcttc gagagtgata acaccaccgc agcgcgttac gtagctggca
     2521 atcaagtaca acccaatgcc atgttcaccg ggctcgtcag cacgcgtact gacccctgct
     2581 caaatatttt gtctcgtaga gactctggaa cgccgcagcc ctgatcggcg acttcaatca
     2641 ccacatcatc gccttcatcg ctgaggaata attcaacgct cttgtttcct tcatcgctac
     2701 gcaggctggc ttcgaaggcg ttatcaagta aattgccaac aatggcggca aactcggtgc
     2761 tatccagtcc tggcggcagt tgcgaaagct ggctaccggg gacgatgatc atttttagcc
     2821 ccagttcgcg ggcgcgctgc actttaccaa aaagcagccc cgccacctgg cgatcggcaa
     2881 acgcctcgcg caggctgtca ataagctgtt gctgggcctg agactccccc tgcaccatcg
     2941 ccagcacgcg atcatactct ttcatctgca acagaccatt gagagtcgac atccaattga
     3001 gatgctcgtg tcgcaatgta cgaaggctct caacgtattg ttttatttgc gtcagttgcg
     3061 cattgagggt ggaaatttcg tctttactgc gaaagctgat aatggctccc agcaaatcat
     3121 cacctgaacg aatagcttcc cgattggcaa taacgctcag accgttaaag ttagccacca
     3181 catcctgacg tttttcatca atctgttcgg taaagaaatc ggcagacctg accacttcag
     3241 caatgggttt acccaaccat tgccgtccag gggaactcag ccccagcatc tttcttgcgt
     3301 tacgattgat ggcggtaatg taaccgtgcg gatccaccgc aatcagccct tcataaaccg
     3361 aactaaacag cgcctcttgc tgacggacca cccgtgcgat ttgctttggc tccatgccca
     3421 tcatctgccg acggatatgc gcggccagga accacgacag caacatcaga atccctaaca
     3481 gcacgacaaa cacacctgcc atcggtaata aaaactcagc ccgccagcta tcgattttac
     3541 tcaccaggta gccaatcgac accacgccga tgacttttcc atcgtcatca aagattggcg
     3601 ttttggcgcg catcgccata cccattgacc ctttcccggt aatgaagtag ctctcccctt
     3661 tctccagcgc gccctgtttg gtgaactgca taggataacc aattttctcc ggattaggat
     3721 ggtaaaggcg gatcgagtgc cggtcgccaa tcaccacata atcaaaatcg gtatctcttt
     3781 gtaatttgtt agcgatggtc gccagccgtt tgtagtcacg cgttttcacc gcagagatga
     3841 cactgtcatt ggaggcaata attttcgcct gattcattgc catgtcgcgt acatgaagcg
     3901 ttaaatagtc ctcaaaactg gccgtaaaat attgcgccag ggctgcaatg acaaatattg
     3961 agaacaccag aatcagcaaa aagatacgca gcggaaatgt cagtctttgg aaaaatgcaa
     4021 actgtttatt ctcgttaagc tgcaacattg ttttccttgc gggttgtccc gagcctcttt
     4081 gacggatgta attaaaatgt tgaatcaaaa tacatagata ttaattatga tcgtggcaaa
     4141 ttttattcat taaatcaatt aaatcactta aacctgcgag tcatttcgta attacattaa
     4201 ttaaaaaact taaaaccatt aaacaaataa aaaaaccact aaccctatgt gaaataaatc
     4261 aaaatttcac gccgaaatac tccttaggat gtatagcgaa aggagaaaaa gatatacctc
     4321 gatcaccccc ctttctccca agtgaaaata aaaggttatc agtttgcaac attgaacaac
     4381 attcgttgca aatcgataac aacatgcacc ttcaggatac tatttattat gttcgtcaat
     4441 gatattttca cccgcgtaaa acgttcagaa aataaaaaaa tggcggaaat cgcccaattc
     4501 ctgcatgaaa atgatttgag cgttgacacc acagtcgaag tatttattac cgtaacccgc
     4561 gatgaaaagc ttatcgcgtg cggtggaatt gccggaaata ttattaaatg cgttgctatc
     4621 agtgaatccg ttcgcggtga aggactggcg ctgacattag ccactgaact gataaacctc
     4681 gcctatgagc ggcacagcac gcatctgttt atttatacca aaaccgaata cgaggcgctg
     4741 ttccgccagt gcggtttttc cacgctgacc tgcgtacccg gcgtgatggt gctgatggaa
     4801 aacagcgcca cgcgactgaa acgctatgcc gaatcgctga aaaaattgcg tcatccaggg
     4861 aacaagattg gctgcattgt gatgaacgcc aatcccttta cgaatggtca ccgttatctg
     4921 attcaacaag ctgcggcaca gtgcgactgg tttcatctgt ttttagtcaa agaagattct
     4981 tcacgcttcc cctatgaaga ccggctggat ctggtgttaa aaggcaccgc cgatattcca
     5041 cgcctgactg tgcatcgcgg ctcagaatac atcatctccc gcgctacgtt cccttgctac
     5101 ttcattaaag aacagagcgt cattaaccat tgttacaccg aaattgatct gaagattttc
     5161 cgtcagtacc tcgctcccgc gcttggtgta actcaccgct ttgtcggtac tgaacccttt
     5221 tgtcgcgtta ccgcccagta caaccaggat atgcgctact ggctggaaac gccgactatc
     5281 tccgcagcgc ccatcgaact ggttgaaatt gagcggctgc gttaccagga gatgccgata
     5341 tccgcttccc gggtacgtca actgctggcg aaaaacgatc tcacggctat cgcgccgctg
     5401 gtccctgcag tcacgctgca ttatttgcag aacctgcttg aacactcccg ctaggacgcg
     5461 gcagctcgtc aaaagacccc cgcatgagaa acaggtgaaa aatgaaaata aaccagcccg
     5521 ccgttgcagg cacccttgag tctggggatg tgatgatacg catcgcccca ctcgatacgc
     5581 aggatatcga cctgcaaatc aatagcagcg ttgagaaaca gtttggcgat gcaattcgca
     5641 ccaccattct ggacgttctc gcccgctaca acgtgcgcgg cgtacagctg aatgtcgatg
     5701 acaaaggcgc actggactgc attttacgtg cacgactgga agccctgctg gcacgcgcca
     5761 gcggtatccc ggctctgcca tgggaggatt gccaatgatt tccgcttcgc tgcaacaacg
     5821 taaaactcgc acccgccgca gcatgttgtt tgtgcctggt gccaatgccg cgatggtcag
     5881 caactccttc atctacccgg ctgatgccct gatgtttgac ctcgaagact ccgtggcatt
     5941 acgtgaaaaa gacaccgccc gccgcatggt ttatcacgcg ctgcaacatc cgctgtatcg
     6001 cgatattgaa accattgtgc gtgtcaacgc gctggattcc gaatggggtg ttaacgacct
     6061 ggaagccgtc gttcgcggtg gtgcggacgt tgtgcgtctg ccgaaaaccg ataccgctca
     6121 ggatgttctg gatattgaaa aagagatcct gcgtatagaa aaagcctgtg gtcgtgaacc
     6181 cggcagcacc ggcctgctgg cggcgattga atctccgctg ggcattaccc gcgcagtgga
     6241 aatcgctcac gcttccgagc gtttgatcgg tatcgccctc ggtgcagaag actatgtgcg
     6301 caacctgcgt acagaacgct ccccggaagg aactgaactg ctgttcgcac gctgttccat
     6361 tttgcaggcc gcgcgctctg cgggtattca ggcgttcgat accgtctatt ccgacgctaa
     6421 caacgaagcc ggattcctgc aagaagccgc ccacatcaaa cagctgggct ttgacggcaa
     6481 atcgctgatc aacccgcgtc agattgatct gctgcacaac ctctacgcac cgacccagaa
     6541 agaagtggat cacgcccgcc gcgtcgtaga agccgctgaa gccgccgctc gcgaaggcct
     6601 cggcgtggtt tccctgaacg gcaagatggt ggacggtccg gttatcgatc gcgcccgtct
     6661 ggtgctctcc cgtgcagaac tttccggcat ccgcgaagaa taaggcaatc aaaatgacgc
     6721 agaaaattga acaatctcaa cgacaagaac gggtagcggc ctggaatcgt cgcactgaat
     6781 gcgatcttgc cgctttccag aactcaccaa agcaaaccta ccaagctgaa aaagcgcgcg
     6841 atcgcaaact gtgcgccaac ctggaagaag cgattcgtcg ctcaggttta caggacggca
     6901 tgacggtttc cttccatcac gctttccgtg gcggtgacct gaccgtcaat atggtgatgg
     6961 acgtcatcgc gaagatgggc tttaaaaacc tgaccctagc gtccagctcc ctgagtgatt
     7021 gccatgcgcc gctggtagaa cacattcgcc agggcgtggt tacccgcatt tatacctctg
     7081 gcctacgcgg tccactggcg gaagagatct cccgtggtct gctggcggaa ccggtgcaga
     7141 tccactctca cggcggtcgt gtgcatctgg tacagagcgg cgaactgaat atcgacgtgg
     7201 ctttcctcgg cgtcccgtcc tgtgatgaat tcggtaatgc caacggctac accggtaaag
     7261 cctgctgcgg ctccctcggc tatgcaatgg ttgatgccga caacgcaaaa caggtcgtga
     7321 tgcttaccga agaactgctg ccttatccgc ataatccggc aagcattgag caagatcagg
     7381 ttgatttgat cgtcaaagtt gatcgcgttg gcgatgctgc aaaaatcggc gctggtacta
     7441 cccgtatgac cactaacccg cgcgaactgc ttattgcccg tagcgctgcg gatgtgattg
     7501 tcaactctgg ctacttcaaa gaaggtttct ccatgcaaac cggcaccggc ggcgcttcgc
     7561 tggcggtaac ccgtttcctg gaagacaaaa tgcgtagccg cgatattcgc gccgacttcg
     7621 ccctcggcgg tattaccgcg acgatggttg atctgcacga aaaaggtctg atccgcaaac
     7681 tgctggatgt gcagagcttt gacagccatg ctgcgcaatc gctggcccgt aaccccaatc
     7741 acatcgaaat cagcgccaac cagtacgcta actggggttc gaaaggcgca tcggttgatc
     7801 gtctcgacgt ggtggtactg agcgcgctgg aaattgacag gcagttcaac gttaacgtgc
     7861 tgaccggttc tgacggcgta ctgcgtggcg cttccggtgg tcactgcgat accgcgattg
     7921 cctctgcgct ttccatcatc gtcgcgccac tggtacgcgg tcgtattccg actctggtgg
     7981 ataacgtgct gacctgcatc accccgggct ccagtgtcga tattctggtc acagaccacg
     8041 gtatcgcagt taacccggca cgtccggaac tagcagaacg tctgcaggaa gcgggcatta
     8101 aagtggtttc cattgagtgg ctgcgcgaac gtgcgcgtct gctgaccggt gaaccacagc
     8161 cgattgaatt caccgaccgc gtcgttgccg ttgtgcgtta ccgcgatggc tcggtgatcg
     8221 atgttgtgca tcaggtgaag gaataagcca tgcacctgct tcctgaactc gccagccacc
     8281 atgcggtgtc aattcccgag ctgctcgtca gccgggatga aaggcaagca cggcaacacg
     8341 cctggctcaa gcaccatcct gttccactgg tctcctttac cgtggttgcg cctgggccga
     8401 ttaaagacag cgaggtcaca cgccgaattt ttaatcatgg cgtgacagcc ttgcgtgcct
     8461 tagccacaaa gcagggctgg caaattcagg agcaggctgc actggtttcc gccagcgggc
     8521 cggagggcat gttgagcatt gccgccccgg ctcgcgatct caagctcgcc accattgagc
     8581 ttgaacatag tcatcctctc gggcggttat gggatatcga tgtcctgacg cccgaaggcg
     8641 aaattctctc ccgccgcgac tattcactgc cgcctcgccg ctgcctgttg tgcgagcaaa
     8701 gcgcagccgt ctgcgcgcgt ggaaaaaccc atcaactgac cgatttactc aaccgcatgg
     8761 aggcactgct gaacgatgtc gatgcctgca acgtcaacta aaaccacaaa gcttgcgacg
     8821 tcattaatcg atgagtacgc cctgctgggc tggcgcgcca tgctgactga agtcaatctg
     8881 tcaccgaaac caggcctcgt ggatcgcatt aactgcggtg cgcacaaaga tatggcgctg
     8941 gaagatttcc accgcagcgc gctggcgatt cagggctggt taccccgttt cattgaattt
     9001 ggtgcctgta gtgcagaaat ggcaccggaa gcggtactcc acggattacg cccaattggt
     9061 atggcttgcg aaggtgatat gttccgcgcc actgcgggcg taaacacgca taaaggcagc
     9121 attttttctt tagggctgct atgtgcggca attggccgtt tgcttcagct caaccagccg
     9181 gttacgccaa caaccgtttg ttctacggcg gcaagtttct gccgtggcct gaccgatcgc
     9241 gaactgcgta ccaataattc acgactgacg gcaggtcaac ggttgtacca gcagcttggc
     9301 cttaccggcg cacgcggtga agccgaagcg ggttatccac tggtgatcaa ccacgccttg
     9361 ccgcattacc tcactctgct ggatcagggg ttagatcctg aactggcatt gctcgatacc
     9421 ttgctcctac tgatggctac caacggcgat accaacgttg catcgcgcgg tggcgagggg
     9481 ggcctgcgct ggctacagcg cgaggcgcaa acattattgc aaaaaggggg cattcgaacc
     9541 cccgccgatc tcgattatct ccggcagttc gacagggagt gtatcgaacg aaatctcagt
     9601 ccaggcggca gtgccgacct gctgatcctt acctggtttt tagcacagat ttaattattt
     9661 aagcacttga taaatttgga aatattaatt ttcggagaac ccgtatgtct ttagcaaaag
     9721 ataatatatg gaaactattg gccccactgg tggtgatggg tgtcatgttt cttatccctg
     9781 tccccgacgg tatgccgccg caggcatggc attacttcgc tgtgtttgtg gcaatgattg
     9841 tcggcatgat cctcgagcca attccggcaa cagcgatcag ttttattgcg gttactattt
     9901 gcgttattgg cagtaattac ctgctctttg atgccaaaga attagctgac ccagcgttta
     9961 atgcgcaaaa acaggcgctg aaatggggcc tggctggttt ttccagcacc acggtatggc
    10021 tggtatttgg cgcatttatt tttgcattag ggtatgaagt ttccgggtta ggtcgtcgca
    10081 ttgccctttt cctggtgaaa ttcatggaca aacgcacgct gacgttgggt tatgcgattg
    10141 tcattatcga cattctgctg gcaccgttta caccgtccaa caccgcgcgt accgggggta
    10201 cggtttttcc ggtcattaaa aacctgccgc cgttgtttaa atcattcccg aacgatccgt
    10261 ccgcgcgtcg tattggcggc tatttgatgt ggatgatggt cattagtacc agtctgagtt
    10321 catccatgtt tgtcaccggt gcggcaccaa acatgctggg tctggagttc gtcagcaaaa
    10381 ttgccggtat ccagattagc tggttgcagt ggttcctctg cttcctgccg gttggtgtta
    10441 tcttgcttat cattgcgccg tggctttcct acgtgctgta caaaccggaa atcacacaca
    10501 gtgaagaagt ggcaacctgg gcgggtgatg aactaaaaac catgggtgcg ctgacacgca
    10561 gagagtggac gctgattggc cttgtattgc tcagcttagg tttgtgggta tttggcagtg
    10621 aagtcattaa tgctactgcg gttggtctgc tggcagtttc gctaatgctg gctctgcacg
    10681 ttgtgccgtg gaaagacatt acccgctata acagcgcatg gaacacgctg gtcaacctgg
    10741 caactctggt tgtgatggct aacggcctga ctcgttctgg ttttattgac tggttcgccg
    10801 gtaccatgag tacgcacctg gaaggattct caccaaacgc aacggtgatt gtactggttc
    10861 tggtgttcta ctttgcacac tacctgtttg ccagcctgtc tgcgcacacc gcaaccatgc
    10921 tgccggttat tctggccgtc ggtaaaggta ttccgggcgt accaatggaa caactgtgta
    10981 tcctgctggt gctgtctatc ggtatcatgg gctgtctgac gccgtatgca accggtcctg
    11041 gggtgattat ttacggctgt ggctatgtga aatcaaaaga ttactggcgt cttggcgcaa
    11101 tcttcggggt gatttacatc tctatgttgc tgttggttgg ctggccgatt ctcgccatgt
    11161 ggaactaata cgttcgccaa acgctgaata aatcatcacg cccaccaggt aagcccacct
    11221 ggcgggcttt tttatgattt aatagatagt attcagtgta tgtatgagtt ccacacccat
    11281 tatgaaagca ttctgccgta acgccgcgtt gctcgcggtt tctctgcttc ccttatcttc
    11341 tgccaacgcc gtagcgttgc aggcaaaaca gtatggcgat tttgatcgct atgtcctggc
    11401 cctctcctgg caaaccggat tttgccagag tcaacacgat cgaaatcgta acgaacgaga
    11461 tgaatgtcgc ctgcaaaccg aaacgaccaa caaagctgat tttctgaccg tacatggtct
    11521 gtggccagga ttgcctaaat cggttgctgc ccgtggtgtt gatgaacgcc gctggatgcg
    11581 cttcggttgc gctactcgcc caatcccgaa tctaccagaa gcacgcgcca gccgaatgtg
    11641 ttcatcgccg gaaaccgggt tatcactgga aacggccgct aaactaagtg aagtcatgcc
    11701 aggagctggc ggacgttcct gcctggaacg ctacgaatat gccaaacacg gtgcctgctt
    11761 tggttttgat ccggacgcat acttcggtac gatggtacgc ctgaatcaag aaattaaaga
    11821 gagcgaagcc ggaaaattcc ttgcggataa ttacggtaaa acagtgagcc gccgtgactt
    11881 tgacgccgcc tttgccaaaa gctggggaaa agagaacgtg aaagcagtta agctaacgtg
    11941 ccagggtaac cctgcgtatt tgactgaaat tcagatctcg atcaaagctg acgccatcaa
    12001 cgctccgctt tctgcaaact cattcttgcc acaacctcac ccaggtaact gtggcaaaac
    12061 ctttgtgatt gataaagcgg gttattaatc ctctgattta caaaacacgc taattcgtct
    12121 tccataacca tgccgattaa gaattataca taatgcgctg cattgaggaa ttccctgaat
    12181 gtgacgcaaa tcacttcaaa gagtgagcaa cagtcagtat catcatgtga gttaaaccct
    12241 cgccgcctga cggtgagggt tttcttttgg gtatgtttat ctgcgacact cgcagtaccg
    12301 acgacatgga gtaaaaatgt ccagaccaac tatcatcatt aacgacctgg atgccgaacg
    12361 catcgatatt ctgctggagc aacccgccta tgctggtttg ccaatcgccg acgcgttaaa
    12421 cgcagagttg gatcgcgccc aaatgtgttc gccagaagag atgccacacg acgtggtgac
    12481 aatgaacagc cgggttaaat tccgcaatct tagagatggc gaagtgcgtg tgcgcacgct
    12541 ggtgtatccg gcaaaaatga ccgatagcaa tactcagctt tccgttatgg ctccggtagg
    12601 tgccgcactg ctggggctgc gcgttggcga ttccattcac tgggaacttc cgggcggcgt
    12661 tgcaacccac cttgaagtgc tggaactcga ataccagcca gaagctgctg gcgactacct
    12721 gctttaatcc catctgcaca agcgataact cgctttgccc agaggatgca ctcgcatcct
    12781 ctttacgcaa aatcagcaat ttttcctggt tctcactgtt cacttttgct gcaaaattat
    12841 tccgtttcgt ttgtgggaat aaccatggca gaagcatttt acatcctgat aggatttctg
    12901 attatggcgg cgattatcgt catggctgtc ctttacctcg aaaaccactc ctgacgatca
    12961 tattgttccc cccggcatcg cattcaccag acctgaaacc gccttctgcg ccgcctctgc
    13021 gctttgtgca ccgggtacca gaatcacctt acggcactcc tcttcacgtt tttcgaaaat
    13081 ctcatatccc cgggcggcct cttcaaacgg catatagtgg gtaacaattt cttctggttt
    13141 cagtaatcct ttctcaatta acggtaataa ttctcccagc catgcgtgaa cgtgggtctg
    13201 tcccatttta aacgtcaacc ctttatcaaa ggcgtcgcca aacaggaaac cgtgaataaa
    13261 tccagcgtag acgcccggta cgctaacaat gccgccacgc ctgaccgccg caatacactg
    13321 gcgcaacgct ttaccgctgc tgccctccag tttcaggtta gtcagcaccg tttccgtggt
    13381 gctgcctttc gcttcaaaac cgacggcgtc tattactgca tccacgcccc ggtgacctgc
    13441 cgtttgttca ataattgact gtgccggatc gctatcttca tcgaaattaa tcgggatcgc
    13501 gccgtagcgg tcagcggcga aacgcaagcg gtagggatga tgatcaacaa caaaaatctg
    13561 ttccgcaccg agcaaccgtg cacaggcgat tgtcaacaat cccacaggac cagcaccata
    13621 gactgcaacg cttgaacctt gttggatctg cacatttttt gctgcctgcc atgccgttgg
    13681 cagaatatca gaaaggaaaa gggctttatc atctgaaagc aaaggcggta ctttaaacgg
    13741 ccccacattc cctttaggga cgcggacata ttccgcctgc ccgccaggaa cgccaccata
    13801 caggtgacta taaccaaaca atgccgcggg agcaggtatc tgttttttat tgagcgcagc
    13861 gcctttaccc gcattggtat tttcgcaggc ggcatattgc tgtaatcgac agaaaaaaca
    13921 gtcgccacaa gcaatgacga acggaattac cacccggtcg cctttttgca aattttttac
    13981 gtccttcccg gtttcaacga cttccccata aattcatgac caaaaatatc gccatgttta
    14041 acctggggta ttttgcctcg ataaagatgg aggtcagagc cacagatcgc cgttaccgta
    14101 atacgcagaa taatatcatc tgcctgttca atgcccggat cgggaacatt ttctacctga
    14161 acgtgatgtg ggccgtgata agtcaatgct ttcatgcaac ctccagagac agttcaaaaa
    14221 agagtcagtc tttaagggta gctatcacag cgggaaaacg ggcggtaccg caggttttgg
    14281 gagaattcct ggtgttcagt gagaatatat taatcacaga aaccgttcgt aaggaaaaag
    14341 aaccagacaa atatcaggga taaatattat aatcatggcg aatgatttgt tcatgattaa
    14401 cagggagaaa gggttatgta taaggcaatc attatgccag ttgatgtatt tgaaatggaa
    14461 ttgagcgaca aagctgttcg ccacgctgaa ttcctcgccc aggatgacgg agttattcat
    14521 ctacttcacg tactacccgg gtcagccagc ctgagcctgc accgttttgc cgctgatgtg
    14581 cgtcgttttg aagagcatct gcaacatgaa gcagaagaac gtctgcaaac gatggtcagc
    14641 cacttcacca tcgatccttc ccgcattaaa caacatgtcc gttttggtag cgtgcgggat
    14701 gaagtcaatg agttggcaga agaactgggg gctgatgttg tagttatcgg ttctcgcaac
    14761 ccatcgattt cgacccatct gttaggttct aacgcctcga gcgtgatccg ccacgccaat
    14821 ctgcctgtac tggtcgttcg ctaacagata aaagtattta tgccgccctg cctggaacat
    14881 cccggcaggg ctttttaatg ccataaaaaa gccgccaggt ttgaccccgg cggctaagca
    14941 attgcaggtg aatcttactt cttcttatgc agttttggtg cgaatcaggt agtcaaaagc
    15001 actcagagag gctttggcac cttcgccagt ggcgatgatg atctgcttgt acggaaccgt
    15061 cgtacagtca cccgctgcga acacgccttt gacgttggtt tcacatttcg catcaatgat
    15121 aatctcgccc atgcggttac gttcgactgc gccttccagc cagttggtgt tcgggagcag
    15181 accaatctgg acaaaaatac cggccagttc gatgttgtga atattgccgc tgacacgatc
    15241 tcgatattcc agaccaacga ctttgctgcc gtcgcctttc acttccgtgg tttgcgcatt
    15301 cagaataatg tcgacgtttt tcaggctgcg cagtttgtcc tgcagaacct ggtcggcttt
    15361 catttctggc gcaaattcca gcagcgttac gtgctcaacg atacccgcca ggtcgattgc
    15421 cgcttccacg ccggagttac cgccgccgat aaccgctacg cgtttgcctt taaacagcgg
    15481 gccgtcgcag tgcgggcagt aggtcacgcc tttagtgcga tactgatctt cgcccggaac
    15541 gttcatgttg cgccattttg cacccgtcgc cacgataatg ctgcgtgctt tcagtaccgc
    15601 gccagaagct gtttcaatct ggtgtagacc accttcaacc gctgccggga tcagtttgct
    15661 ggcgctctgg ctgtcgatca catcaacgtc gtattcatca acgtgaactt tcagtgcgcc
    15721 tgccaacttc tgcccttcag tcttcggtac agaaatgtag ttttcgatat caacggtatc
    15781 gaggatctga ccaccaaaac gttcgcccat cagaccggta cggatgcctt tacgtgcgga
    15841 gtaaattgct gccgctgcac ccgccgggcc ggaaccaacg attaatacgt cataagcatc
    15901 acgcttgttc agctcttctg ccgcacgttt ttccgcgcca gtatcaattt tggcaacgat
    15961 ttcagtcaac gtcatgcggc cctgaccaaa ctctttcccg tttacgaaca ctgccggaac
    16021 gcccatcacg ttgcgatcgg tgatttcgtt ctggaaggtg ccgccgtcaa ttgcagtgtg
    16081 cttgatgcgc gggttcaata cgctcatcag gttcagcgcc tgcaccacgt ccgggcagtt
    16141 atggcaagag agcgagtaat aggtttcgaa ttcaaaatca ccgtcaatat ggcgaatctg
    16201 ctccagcaga gactgcgctt ctttcgacgg atgaccaccg gtccacagca acgccagtac
    16261 cagcgaggtg aactcgtggc ccagcgggga gcctgcaaaa cgtggtccct ggttggaacc
    16321 tgggttggtg atcaggaaag acggcttacg cactggcaag ctgttatctt ctttaaaggt
    16381 gactttgtct gacagttctg cgatttcagc caacagttcc ttgatttctg ccgatttagc
    16441 gctgtcatcc agcgtggcaa ttaactcaac aggcttggtc aatttctcaa ggtaagcctt
    16501 gagttgagtt ttcatatttg tgtcgagcat gtttatctcc tgggctttaa acatcatcat
    16561 gcaagcggcc tctgcggcta cctggatgca gcttgcatca tggtgcgggg aagaaaatcg
    16621 ggtgccgcag cacccgaaga attaggtgaa tttccgaagt atttcgcgtc agagcaaggc
    16681 gactagcctg tgaatccccg ggagcttaca caagtaagtg accggggtga gcgggcgacg
    16741 ccaacgccgc tatggcgtga aagacgaagg aaatttagat tttaccaacc aggtccagag
    16801 acggagccag agttgcttca ccttctttcc atttagccgg gcaaacttca cctgggtgag
    16861 aagctacgta ctgagctgct ttgattttac gcagcagatc agacgcgtca cggccaatgc
    16921 cttcagcggt aacttcgatt gcctggatga taccctgcgg gtcaacaacg aaggtcgcac
    16981 ggtcagccag accttcatct tcacgcatgt tgtcgaagtt acgggtcagg gcgccagtcg
    17041 ggtcgccgat catcgcatat ttgattttag cgatggtttc agagctgctg tgccatgctt
    17101 tgtgggtgaa gtgagtatcg gtagatactg cgtatacgtc tacgcccagt ttctgcagtt
    17161 cttcgtagtg gtcagcaacg tcacccagtt cggtcgggca tacgaaagta aagtcagccg
    17221 ggtagaagaa gaagacgctc cagcggcctt cggtatcttt ttcggtgatt tcgatgaatt
    17281 cgccgttttt gaatgcctgg tttttaaaag gtttaatttt ggtgttaatc aaggacatct
    17341 atacttcctc cgtgttttcg atgagatgta aggtaaccaa tttttgccga ttcggctaat
    17401 gcgtttccat tatcaaatcg ataagtttta ccttacaacc ttcgtaagac aactttgtga
    17461 actttgcacc gccagcggcg ataaaaagta aaatggcccc ctttccaggt gaccgtgtaa
    17521 tctgaactac cctttggtat cttcagcgcc tgcctgcagg ctgaggtgtt gcccttacaa
    17581 atgcaacaac gacatggatt acaacaccct cataaacaaa gggcaatcac ctgatctaag
    17641 ctcttaccta tgacagtgat aggttatgcc ttttactcga cttttgcact gactgaaaag
    17701 aacaaattaa tgttaaaaaa gatactttta ctggctctgc ttcctgcaat cgccttcgca
    17761 gaggaacttc cttccccagt aaaagcgatt gaaaaacagg gcattacaat catcaaaacg
    17821 ttcgatgccc ccggaggaat gaaaggttat ctcggaaagt atcaggatat gggcgtcacc
    17881 atctacctga ctcccgatgg taagcacgct atctctggtt acatgtacaa cgagaaaggt
    17941 gaaaacctga gtaacacact tatcgaaaaa gaaatttacg caccagccgg acgcgaaatg
    18001 tggcaacgga tggaacaatc ccactggctc ctcgacggta aaaaagatgc gccggtcatt
    18061 gtctacgtct tcgccgatcc gttctgccca tattgtaaac agttctggca gcaggcgcgc
    18121 ccgtgggtag attctggcaa agtgcaatta agaacattgt tggttggggt tatcaagcca
    18181 gaaagcccgg cgacagcagc ggcaattctt gcctccaaag atcccgcaaa aacctggcaa
    18241 caatatgaag cctctggttg caagcttaag ctaaacgtgc ctgcaaacgt aagtacagag
    18301 caaatgaaag tgttaagtga caatgagaaa ctgatggacg atctgggggc aaatgtcacg
    18361 ccggctatct attacatgag taaggaaaat acgctgcaac aggccgtggg gttgcccgat
    18421 cagaaaacgc ttaatatcat tatgggaaat aaataaaagg taattcatgg atggttgaac
    18481 tatatccctg cttttcaacc atcctaaaaa agcatgaatt gctcgctcta ccctaagtgg
    18541 ctgtgctatt ttagaaaaac ttaatcctga gttaagcaaa aaaacataat caataaaaat
    18601 acatacgcaa aaaacattta ttaagtgaat ataccatgga agaaaaatat aaaccggagt
    18661 agtgtatggc caatctctac gacttgaaaa agttcgactt aaatcttctt gtcatatttg
    18721 agtgtattta ccagcattta agcattagca aagctgctga atcactatat atcaccccat
    18781 cagccgtgag ccagtcgtta caacgcttac gtgcgcaatt taatgaccct ttattcattc
    18841 gctcaggaaa aggtatcgcg ccgacgacaa cagggttgaa tctgcatcac catcttgaga
    18901 aaaacctcaa aggccttgaa caaacaatta atatagtcaa taagtccgaa ctaaagaaaa
    18961 actttattat ctacggccct cagctcattt cttgctctaa taatagtatg ctgatccgct
    19021 gcctacggca agatgcctct gtcgagattg agtgccatga tatcctcatg tcagcagaga
    19081 atgctgaaga actgttggtt caccgcaaag ccgatctggt gatcacccaa atgccagtga
    19141 tcagccgttc cgtcatttgc atgccattgc atacaattcg caatactcta atttgtagta
    19201 ataagcatcc gcgaattact gacaatagca cttatgaaca aattatggca gaagagttca
    19261 cccaattaat atccaaatca gcaggagttg atgatattca aatggaaata gacgagaagt
    19321 ttatgaatcg caaaatttca tttcgtggtt cctctttgct gacaataatt aatagcattg
    19381 cagttacaga tttactggga atagtcccct atgaacttta taactaccac cgtgactttc
    19441 ttaatttaaa agagataaaa ctagaacatc cgttaccttc aataaagctt tacatttcct
    19501 ataataaatc ttctttaaat aatttggtgt tctcccgatt tattgaccgt ctaaatgaga
    19561 gtttttgata tataactaca gacagctact ataactttca tctatttatt cacagctcga
    19621 atgaaaaata aaacactctg ctttacagtg tttactttat aattaacagt cttatctcac
    19681 tgttcaccct gtaacgttca gagagcgtct tcagatgtct atttataaaa ttcctcttcc
    19741 actcaatatt ctggaagctg cacgagagcg tattacctag acactcaaca ccctaccccg
    19801 cgtttgtgtc tcattttctg gcggcaaaga ctctggttta atgctgcatc tgacagccga
    19861 acttgcccga caaatgggca aaaaaatctg cgttttgttt atcgactggg aggc
//
LOCUS       DADMEQ010000069        19900 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.069, whole genome shotgun sequence.
ACCESSION   DADMEQ010000069 DADMEQ010000000
VERSION     DADMEQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19900
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.069"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            7..375
                     /locus_tag="JNZ05_002486"
     CDS             7..375
                     /locus_tag="JNZ05_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565683.1"
                     /translation="MFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSER
                     PIWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPIRCAFTGALTLYYYFIGFF
                     GILAAIAITLVWQRHTRFFH"
     gene            complement(411..1367)
                     /locus_tag="JNZ05_002487"
     CDS             complement(411..1367)
                     /locus_tag="JNZ05_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAY8565684.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IITALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1367..2608)
                     /gene="clsB"
                     /locus_tag="JNZ05_002488"
     CDS             complement(1367..2608)
                     /gene="clsB"
                     /locus_tag="JNZ05_002488"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HAY8565685.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQKRIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQCEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2605..3366)
                     /locus_tag="JNZ05_002489"
     CDS             complement(2605..3366)
                     /locus_tag="JNZ05_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY8565686.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3499..3909
                     /locus_tag="JNZ05_002490"
     CDS             3499..3909
                     /locus_tag="JNZ05_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565687.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(3871..4977)
                     /locus_tag="JNZ05_002491"
     CDS             complement(3871..4977)
                     /locus_tag="JNZ05_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8565688.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(4988..6121)
                     /locus_tag="JNZ05_002492"
     CDS             complement(4988..6121)
                     /locus_tag="JNZ05_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8565689.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6114..7850)
                     /locus_tag="JNZ05_002493"
     CDS             complement(6114..7850)
                     /locus_tag="JNZ05_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8565690.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(7843..8841)
                     /gene="hlyD"
                     /locus_tag="JNZ05_002494"
     CDS             complement(7843..8841)
                     /gene="hlyD"
                     /locus_tag="JNZ05_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HAY8565691.1"
                     /translation="MMKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRT
                     VNLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLML
                     AGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQA
                     TLKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTR
                     AVEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQ
                     IGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(8841..9512)
                     /gene="cecR"
                     /locus_tag="JNZ05_002495"
     CDS             complement(8841..9512)
                     /gene="cecR"
                     /locus_tag="JNZ05_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HAY8565692.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEETERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHSHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9741..10928
                     /gene="rhlE"
                     /locus_tag="JNZ05_002496"
                     /pseudo
     CDS             9741..10928
                     /gene="rhlE"
                     /locus_tag="JNZ05_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(11135..11617)
                     /gene="ybiA"
                     /locus_tag="JNZ05_002497"
                     /pseudo
     CDS             complement(11135..11617)
                     /gene="ybiA"
                     /locus_tag="JNZ05_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
     gene            11737..13887
                     /gene="dinG"
                     /locus_tag="JNZ05_002498"
     CDS             11737..13887
                     /gene="dinG"
                     /locus_tag="JNZ05_002498"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HAY8565693.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            13915..14877
                     /gene="ybiB"
                     /locus_tag="JNZ05_002499"
     CDS             13915..14877
                     /gene="ybiB"
                     /locus_tag="JNZ05_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HAY8565694.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEVATISDGLARVNQAF"
     gene            15018..16103
                     /locus_tag="JNZ05_002500"
     CDS             15018..16103
                     /locus_tag="JNZ05_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HAY8565695.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(16332..16592)
                     /locus_tag="JNZ05_002501"
     CDS             complement(16332..16592)
                     /locus_tag="JNZ05_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY8565696.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(16884..17123)
                     /locus_tag="JNZ05_002502"
     CDS             complement(16884..17123)
                     /locus_tag="JNZ05_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HAY8565697.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNR"
     gene            complement(17197..17910)
                     /gene="ybiX"
                     /locus_tag="JNZ05_002503"
     CDS             complement(17197..17910)
                     /gene="ybiX"
                     /locus_tag="JNZ05_002503"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HAY8565698.1"
                     /translation="MWGAGPTFWRTCMMYHIPGVLSPQDVAHFREQLEQAEWVDGRVT
                     TGAQGAQVKNNQQVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNN
                     ETYGFHVDGAVRSHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGD
                     LVLYPSSSLHCVTPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENE
                     EILSLLNLYHNLLREWSEI"
     gene            complement(17916..19900)
                     /locus_tag="JNZ05_002504"
                     /pseudo
     CDS             complement(17916..19900)
                     /locus_tag="JNZ05_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         4616 a         5225 c         5373 g         4686 t
ORIGIN      
        1 ggcgcgatgt ttttaatcag tatgctggcc ggtttattat ttaatgttga tcctggttct
       61 caccgtttta tcattatgat gacgttgaca gggttggccc tggtaatcat cgtgaatgcg
      121 gcattaatga gtgaacggcc catttgggta ataagttgct taatgattgt gttatggcca
      181 ggcattatct cgcatggacg aaataagctc cttgaactgg caggaaaatg ccatagtgaa
      241 gagttgtgga gtccgattcg ttgcgctttt acaggtgcat taacactcta ttactatttt
      301 atcggcttct ttgggatact tgccgcgata gctataacgc ttgtctggca aaggcatacg
      361 cgtttttttc attagtcgta aatgcagata ctaaagaact taactaaact tcacatcgcc
      421 gcttcatttt tcgcccgtag cttcttcgct tggctttcca gtagcaggta acagatcagc
      481 gccagcagca gcgggataaa gtaatacagg acgcgataag cgagcagggc ggtgataatg
      541 ctccctttcg aggtatgctc cccagccagt agtgcgataa acaccgcttc cagcacaccg
      601 atccccgccg gaatatgcac gatgacgcca gcaatactac taaccagtaa cacgcccagt
      661 acaaagaaat agttcacgct ttgaccaagt aacagccaga taatcgcccc cattaccatc
      721 cagttaacgc tggaaatcag catctgagcg agggcgaatt tccatgaagg cagcaccagc
      781 ttttgccctt tgatggtcat atggcggtgc ttcgcgaaag cgcaaaacca caaataaacc
      841 gcgataatca tcagtaagcc aatgccgaga atacgcagcg tagtttgatc gacataccag
      901 tgatccggca actccaccac gcctgcggta aagataatcc ctgccagtaa aatatagccc
      961 agccagttgg tggtaatact gagcgaggaa atccgcgtaa tagtgctgcc cggtaaccct
     1021 agtcgggagt acaaacgata gcgcatacca atgccgccga cccaggtact gagcgtcagg
     1081 ttgaaggcgt agcagataaa cgacaccagc atcacctggc gcttcgccag tttgtgaccg
     1141 cagtaaaagc gggcgagcag gtcatagcag ccgtaaatca gatagctgac gaccaccagc
     1201 ccgaccgcac taagcagcgc aacgcgattg tagtcgcgga tgaccttcca gacctcttcc
     1261 cagtccactt ttttggcgta gaccaccagc aacacaatca ccgcgataaa aaacagccag
     1321 gtgaggatct tctttgctaa gcgccagcgc gggtgtgatt tactcatcag ggttttaccc
     1381 ccgtgttttc agtttctacc cgatcctgcg tttccattgt cggttgtgcg gggggatcaa
     1441 cctgcgtcag acgtggtgtg tgtgccggaa gccagccaac cagcgccggg aagtggcgta
     1501 aaaagtggaa cgccagcacg cttttggtca ggttccacca ggtgcgtttg ggcagcatgg
     1561 tttcatccac ctgctgacaa tctgcggcaa taatgccgtt cagattatcg cgcagcgtct
     1621 ggttaaaatg acgatcgtgg atgatgacat ttgcttcgag attcagtgac aaactgagcg
     1681 gatcgagatt actggaccct actgtcgccc agtgatcgtc catcaatgcc actttgccgt
     1741 ggagcgggcg gcggcggtac tcaaaaacct gaacgccgcc tttaaccaga tagttataca
     1801 gcaagcgcgc accgactctg acaatcggca tatccggttc gcactgaatg atcagtttga
     1861 tccgcacccc gcgccgtgcc gctttacgca aggcgtgtaa aaatcgatag ccggggaaga
     1921 agtaggcgtt ggcgataatc acttcccgcc gcgcctgagt gagcattttc agataatggc
     1981 gttcaatatc atcgcgatgt tcttcgttat cgcgccagac cagcaatacc tgcgcttctc
     2041 ccggctggcg gttctcttcc gctttgtgat gacgtcgcca ccagcgtcgt gccgcgctct
     2101 gtccaggcag gttttccagc tcaaactgga gaatatcttc gacaatcggc ccttcaaggc
     2161 gtaccgcgta atcctgttta gcctctggac cgtagctgga catatgctcg gcggagtaat
     2221 tcagcccgcc aataaaggcg atacgcgcgt cgatcaccac aattttgcga tgcatccggc
     2281 gaaacacatt ggtgcgcata ccaaaaaggc gagggcgggg atcgtagtag cggaacacta
     2341 cgccagctgc cgtcagttca ttgacaaact catcgctgag atccggcgaa ccgtagccat
     2401 ccagcaagac ttccgcttta accccgcgtt gcgctgctgc cagtagtgcc gcatgcagct
     2461 gtttgccgac gttatcctca aaccagataa acgtttcaag aatgatgcgt ttttgtgcct
     2521 cgccaatcgc cttaaacacc gcgggataat attgctcgcc gttttccagc aactggatct
     2581 tattgccttc gcgccagcta catttcataa atgaatctcc gcacttaaag gggcatgatc
     2641 agaaaggtgt cgccatgtcc gcagcggcaa cgcggttggc gcgctggcgc tggcattttt
     2701 gacgtagatc ctgtccagtc gtagtagagg aaattgcacc ggaaacgtgc gcgccgggcg
     2761 tccgtgggcg cgggtaaaaa tctcatccag tccggcctgc acttttaacg gatgattagc
     2821 tttttgccgc cagtcattga aatcacccgc caccaatacc ggttcgccgt ccggtagctc
     2881 attcacccat tcggcgagca tcgcaagctg cgcctgacgg tgcgcctcac gcaggcccag
     2941 atgtacgcac atcacatgaa tcgcttttcc ggtcatcggc ggcacaatgc ggcagtagag
     3001 cacgccgcgc ttttccgcac catcgaccga aacatcgcga ttctcataat gttcaatggg
     3061 ataacgcgac agtacggcgt tgccgtgatg cccttccggg tatacggcat tgcgaccgta
     3121 ggcaaaatcg ctccacatag tgtcggcgag aaactcgtag tgcgaggtat cgggccagtt
     3181 ttccacatgc agcggatgaa cttcgtgcgc gcccatcact tcctgcaggc aaacaatatc
     3241 ggcgctgacg gtacgcacgg catcgcgaag ttccggcaaa atgaagcgtc ggttaaacgc
     3301 ggtaaagcct ttgtgaatgt tgatggtgag caccttgaac gaaaattgtt gtgtttgatc
     3361 gggcataatt ttcctgtctt tgcctcttat ctcattgaaa tagtgtagtc ggcgtcacaa
     3421 aaaggtgcgg tcttacggaa ttttccgtaa agttcggtac tctgagtaag tagagataaa
     3481 ttcttcagga gagaagccat gaagtggcaa caacgtgttc gtgtcgcaac gggtctaagt
     3541 tgctggcaga ttatgttgca tttactggta gtggcgctgc tggtggtggg ctggatgagt
     3601 aagactctgg ttcacgtcgg cgtgggatta tgcgcactgt attgtgtcac ggtagtgatg
     3661 atgctggtgt ttcagcgcca ccccgagcaa cgctggcgtg aggtggcaga cgtgctggaa
     3721 gagctgacca cgacctggta ttttggcgca gcgctgattg tgctgtggct gttgtcccgc
     3781 gtactggaaa acaacttttt gctggcaatt gcagggctgg cgatccttgc tggcccggcg
     3841 gtagtgtctt tgctggcgaa agataagaag ttacatcacc ttacgtctaa acatcgcgta
     3901 cgccgctgac cctgtcgtgg ccgttatcac cagtagcggc cacaaactat tccacacaat
     3961 atccagactc gcatccttca aataaatctg cttggtaatg tccgtaaagt ggcgaatagg
     4021 gttaatccac gtcagatttt gcagccacac tggcatgttt tccaccggcg aaacgtaacc
     4081 ggaaaggaga atggcgggca tcataaaaac aaacacgccg ataaacgcct gctgttgtgt
     4141 cgagcagagt gatgaaatca acagaccgaa tcccaccagc gataaaccgt aaatcaccat
     4201 cgtaaagtag aacagcgcta gcgatccggc gaaggggatt tgatacgccc agataccaat
     4261 cgccagcaca atggtggcct ggaaggtggc gacaattaac gccggtacgg ctttgccgat
     4321 gaagatctgc caggtggtga gcggcgaaac cagtagctga tcgagcgtac cttgttcacg
     4381 ttcgcgggcg acggaaagtg aagtgacgat cattacgccg atagtggtga tcatggcgat
     4441 cagcgacggc accacaaacc atttgtagtc gagattcggg ttataccagt tgcgtaccac
     4501 cagctcgctg ttgttgggtt tcggttttcc ttccagcagc tcctgctgat aatttttgac
     4561 gatctgttgc aggtagttgg cggcaatttg cgcactgttg gagttacgcc cgtcgaggat
     4621 caactgcaac ggcgcggtct ggaaggtatc cagtttgcgc gagaagtcag ccgggaaacg
     4681 caccagtagt aacgcctttt gtgtgtcgat ggtcgggcgg atctcctgtg ggcttttcag
     4741 cagcagcaca tgtgtaaagg cgctggcgcg ggcaaaacgt tgagtaagtt ccaccgaatg
     4801 ctcgccgtta tcttcatcgt agatggcgat ggtggcgtta gtcacttcca gcgtggcggc
     4861 gaacgggaac aggatcacct gaattagcac gggtaaaatc agaatcgcgc gggtttgtgg
     4921 ttcgcgcagc aacgactgca actctttgcg gattaacgtc cataagcgat gaaacatgct
     4981 cttctcccta atccagccga cgtttggttt tcagccacgt caggccgata aacatcaccg
     5041 ccgaagcgat caaaaacagc acgtttacca ccagcaccac tggaatattc ccggcgagga
     5101 acaggctttg cagggtgctg acgaaataac gagcgggaat aatgtacgtc accgcgcgga
     5161 tcaccgcggg catactgtcg atctgaaaaa taaagccgga aagcataatc gacggcagaa
     5221 aagcggcgtt cagggcgacc tgagcggcat tgaactggtt gcgggtaatc gtggaaatca
     5281 gcagccccat tcccagggta ctgagtaaaa acaggctgga gataaaaaac agaatcagca
     5341 gcgaaccgcg atacggcacg ccgagaataa acactgacac cagcatacac agcaacatcg
     5401 ccagcatccc gagaaagtaa taagggatca gcttacacag cagcagttcc gtgcgggtaa
     5461 tctccgtaga gagcagagcc tccatggtgc cgcgttccca ttctcgcgcc accaccagcg
     5521 aggtgagaat cgcgccgatg accgtcatga taatggtcac cgcacccggg ataataaagt
     5581 gctggcttat cgccgccggg ttaaaccagt agcgggtttg tacatcaata agcggttcaa
     5641 aagtctgccc gttgtcctcc gctcgctgca tttgccagat ctgccagatc ccttcgacat
     5701 acccctgcac aaagttagcg gtattcggct cactgccgtc ggtgatcacc tgaatcggtg
     5761 cggtggcgtt ggcgcgctcc atctgttcgg cgaaatccac gggaataacc accagaccgc
     5821 gaattttccc cgactgcatt ttggcgatca gttcctgacg gttatcgctg atggtggcgt
     5881 cgatgtaggg cgaaccggtc atggtgtggg tgaaatccag cgccgcttcg ctacgctgtt
     5941 ccagcaaaat cccgacccgc agcttgctgg agtcgaggtt aatgccgtaa ccaaaaataa
     6001 acagcagtag cagtgggatc actaccgcaa tcagccagct actcggatcg cgaacgatct
     6061 gccgcgtctc tttgacgcac agcgcccgca cgcgatgcca ggaaaggatc gggttactca
     6121 ttgctatgct ccttatccca gtcgtggatc aactgaataa aggcttgctc catggtggga
     6181 tcgggttgct catcgttagc tgactgtgct ttcaaatcgt ccggcgtacc gctggcgatt
     6241 aatttcccac ggtacaccag gccgatgcgg tcgcaatatt ccgcttcatc cataaagtgg
     6301 gtggtgacca tcaccgtgac gcctttctct accatgctgt tgatgtgcag ccaaaattca
     6361 cggcgggtga gggggtcaac gccggaagtc ggttcgtcga gaaagagaat atccggttca
     6421 tgcatcagcg aacaggccag cgccagccgc tgtttaaaac ctaatggcag ttcatcggtg
     6481 gcgtgtgagg caatactttt caggccaaat gcctcgctca tacgggagat tttttcgttc
     6541 tgcgcccgac cgcgtaagcc atacacacca gagaaaaagc gtaaattctg ttcgaccgtc
     6601 aggttgccgt agagcgaaaa tttttgcgcc atatagccga gatgctggcg cgctttaccg
     6661 gaactctctt tcagatccat ccccagcacc agcgcctggc cggaagtcgg caccagcaaa
     6721 ccgcacatca tcttaaaggt ggtcgattta cccgcgccgt ttggccccag caaaccaaaa
     6781 atctccccac gtttaacggc aaagttgacg tgatcggtgg cggcaaaatc accaaatttc
     6841 ttggtcagtt ctttcgcttc gatcaccgtc tcgccgggtg tgccttctac cgtatgcaat
     6901 attgcaccca gcggcgattc tgaggttccg gcaccgccca gcaaatcaat aaacgcatct
     6961 tcaaaacgcg gcgtagtttc gttgatgttg atttccggca tcccgtcggc atggcgaata
     7021 tcgtctggtg tggcctcttt tttgaggatc agacgtaccg atttcccctg aatcatgccg
     7081 tcgctgacct gcggcagttt caaggcgcgt tgcaacagtt tgcggttgcc ctcgtgtgga
     7141 ctggtcatca gaaagctgcg tccggccatg gtttgtgtca gggcttttgg ttctccctga
     7201 tacagcaact cgccttcgtt catcagtaac acgtcacggc actgctcggc ttcgtcgaga
     7261 tacgaggtac tccagaggat taacatccct tcgcccgcca gctcatgcac catctgccac
     7321 agttcgcgcc gtgagatagg gtcaacgccg acgccgggtt catcgagcag caacactttc
     7381 ggttcgccca ccagggtaca ggccaggccg agtttttgtt tcatcccacc ggagagcttg
     7441 cccgccaggc gtccggtaaa cggcccaaga gacgtaaact ccagcaggcg agcaaaagtt
     7501 tgcttacgtg cctcgccggt gacgctgcgc aaatccgcgt acagattgag gttctccatc
     7561 accgtgagat cttcatacag accaaatttc tgcggcatat agccgagcac ggcgtgcagc
     7621 gcgccgtcgt ttttgatcgg atcaaagcca atcaccgtgg cactgccgct gtcgggtttc
     7681 agtaatcccg ccaacatccg catcagcgtg gttttacctg caccgtccgg ccccaccaac
     7741 cccgtcacat aaccggcgtg aatggtacaa tcgagcggcg cgacggcggg cttgtccatg
     7801 cccggaaagc gtttttccag gccgttcagc gtgataacgg catcattcat gtcctgcctc
     7861 gtcaccgaat tgtaccgtca ctggcattcc ctggcgtaac gcatcatcgg cgtcggtcac
     7921 cacaatacgc aggcgataga cgaggtcggt acgcagatcc ggtgtttcga cggttttcgg
     7981 ggtaaattca gcagtcggcg aaacgaaacc aatctgcccg tgatacggct tgtccgggcg
     8041 accatcggta taaagcagca ctttgcgccc cggctgggcc tggtcaagat tccgttcatc
     8101 aacataagcg cgcacccaca ccggacgcgt tagtgaaacg gtaaacaccg tgccaccttc
     8161 attgagaacc gtgcctggct ctaccgcgcg cgttaacagc gtgccatcag acggtgctat
     8221 caacgttgag tcctgtaaat tcagctccgc ctgcgccagt tgcgcctgcg cctgttcgag
     8281 gctggctttc gcctgagcga tatcctgttc acggttaccg gaacggtact gacgcaattt
     8341 atcctgtgct gatttcagcg ttgcctgtgc ctggtcgcgc gaggagcggg cattttccag
     8401 gtcatttgcc gaaatggtgc ggcttttcca cagtccttgc tggcggttat agaagttctg
     8461 cgcatagtca taggcggctt gcgcctgttt caccgctgcg gcggcctgag cgatttcttc
     8521 atcgcgatac ccggcaagca tcagatcata ctgcgcctgt gccaccgaga cgcccgcttt
     8581 cgcctgcatc agggcaatct catacggctt gtgatccagt tcgcccagca cctggcccgc
     8641 tttgatagca tcaccttcgt ccaccgccag cgattcaacg cgccccccaa cacggaaact
     8701 aagattcacc gtacgaatat ccacgttgcc atacagcgtc aggccgttat cctggcggct
     8761 ttgataccac cagtagcctc cggcaaccac ggcggcaagt accactaccg ccagtccgat
     8821 cacgacaggt tttttcatca ctacaaactc ctttgcgata atccttgcaa aatcaggtcg
     8881 atatgacagg tcaccgtctg gttgatcagt tcggtctttt cttcatcgaa cgcggtccag
     8941 ccggtacgta acagaattgt ttctttgcca agacggaacg ccagaatctc accaatcaac
     9001 gcatgggaat gaaggatcat gcgggtgtca ttagcgtcgc agccggtcca ggcggcaatc
     9061 aggcgtgtca ggtggctgtg tagcggacta atcacctgtt cgtgcaccag gtggtaggct
     9121 gccgtgggag agagctgctc acgggagata aacttgctga ggttgacggt gtcatcctgg
     9181 gtgagcagct taatcatgtt cctgcaggcg cgaaggatca gttcacggat ggcagcccga
     9241 tcaggctgtg gttgggcgaa caagcgttcg gtttcctcgg catgcggacg gaactgctcg
     9301 ccaataaaat cggcaatcca ctgggcgcag gcgaggtaca aatcttcttt cgaaccgaag
     9361 tagtaggtga tggcagcgat attctgcccg gcctgggcgg ctatctcgcg ggtggtggcg
     9421 ttcattccat attcaccaaa ctgcgccagt gcggcagcaa tcagctgttt tttcgcctgt
     9481 tcacccttga ttgtcatggc aggattattc atcgcacagt ccattcttaa tcaaatgatt
     9541 gattaagatt atgactccat atgggagttg tccagtatgg ctaagaattt tagctacgcc
     9601 agtcacaggg ataatttatg cgctgcgtca caaaaactgc tacactccgc tccctcatga
     9661 cattgtggtt tttgtcattt tccttttcag tatctccctg aaaactacac cggtaacggt
     9721 cggggcggtt cggagtagtt atgtctttcg attctttggg tttaagccct gatatcctgc
     9781 gcgccgttgc cgagcagggt taccgtgaac ccacccctat tcagcagcag gcgatccctg
     9841 cggtgctgga aggccgcgac ctgatggcta gcgcccagac cggcaccggc aaaacagcgg
     9901 gctttacgct gccgctgttg caacacctga tcactcgcca gccgcacgcc aaagggcgtc
     9961 gtccggtacg tgcgctcatt cttaccccga cccgtgaact ggcggcacag attggcgaaa
    10021 acgtccgtga ttacagcaaa tacctgaaca ttcgttcgct ggtggtgttt ggcggtgtca
    10081 gcattaaccc acaaatgatg aagttgcgcg gcggcgttga tgtgctggtg gcaacgccgg
    10141 gacgtttgct ggacctggaa catcaaaatg cagtgaagct ggatcaggtt gaaatcctcg
    10201 tcctcgatga agctgaccgc atgctcgaca tgggctttat ccacgatatc cgtcgcgtgt
    10261 taacaaaact gccagcgaaa cgccagaacc tgctgttctc tgcgaccttc tctgacgata
    10321 ttaaagccct ggcggaaaaa ctgttgcaca acccgctgga aatcgaagtg gcacgccgca
    10381 ataccgcgtc tgatcaggtg acccagcacg ttcactttgt cgataagaaa cgcaaacgcg
    10441 aattgctgtc gcacatgatt ggtaaaggga actggcagca ggtgctggtg tttacccgta
    10501 ccaaacacgg cgctaaccat ctggctgagc agctcaataa agatggcatc cgtagtgtgg
    10561 cgatccacgg gaataaatcg caaggtgcgc gtacccgtgc gctggctgat tttaaatcgg
    10621 gtgacattcg cgtactggtg gcgaccgata tcgctgcgcg cggtctggat atcgaagagt
    10681 tgccgcacgt cgtcaactat gaactgccaa acgtaccgga agattatgtc caccgtatcg
    10741 ggcgtaccgg tcgtgcggct gctaccggtg aagcgttgtc gctggtgtgt gttgatgaac
    10801 acaaactgct gcgtgatatc gaaaaactgc tgaaaaaaga gatcccgcgc attgcgattc
    10861 cgggctatga gccggacccg tcaatcaaag ccgaaccgat ccagaacggt cgccagcaac
    10921 gtggcggcat caggctgatg aacaaacgca aaactgcctg atgcgctacg cttatcaggc
    10981 ctacgtgaac tctgcaatat attgaatttg catgcttttg taggccggat aaggcgttta
    11041 cgccgcatcc ggcatttcac aacaagcact tgtcagcaat ttgagaacac gggaaaaaat
    11101 ttatctgttt taccgcccat aagccaccga gacgttactt ctctatagcc aattgttcgc
    11161 gcaactccat taaaaggtag cccagtcgat tcttgccctt accattacca ccgtctcccc
    11221 agtaagcatc gttttccgta tgctcaacca gttttgcggg cgcggttgcc agcaagagca
    11281 cacgcagttc tgcatgctgt tcgaatttag cgcgaagtgc tttacgcatc acctgttctt
    11341 taaccgactc ccagttttta cgaagtggct tggaacgatt acgccccatg cgagcggcga
    11401 ccatggggga agaaacccga cggatctctt cgcggtattt ttcatcaagg aatttttgtg
    11461 cctgaaaata gtgttcggag gtgggctagg tattcccgtc aaccttgatg ggcctggcgg
    11521 caaagttaga aaaatcaccg tagtcatcgc tggtgctgta gaaatttatg atggtgtctt
    11581 gcatgacgtg ttggatcctt tgtgctcgaa cgggcattaa accgcattat gttggtggtt
    11641 attgcgagcc gctttccaga aacagaaaaa ccattacccc tgaaaaccga aaaatgccac
    11701 aatattggct gtttatacag tatttcaggt tttctcatgg cattaaccgc cgcgcttaaa
    11761 gcgcaaattg ccgcctggta taaggcgctt caggaacaga tccccgactt tattccccgt
    11821 gcgccgcagc ggcagatgat tgcggacgtc gccaaaacgc tggccggaga agaagggcgg
    11881 catctggcga ttgaagcccc caccggcgtt gggaaaacgc tctcctattt gatccccggc
    11941 atcgccattg cccgcgaaga gcaaaaaacg ctggtggtga gtaccgccaa cgtggcgttg
    12001 caggatcaga tttacagcaa agatttaccg ctgctgaaaa agatcattcc cgatcttaaa
    12061 ttcactgccg ctttcgggcg tgggcgctac gtttgtccgc gtaatctgac ggcgctcgcc
    12121 agcactgaac ccacgcaaca ggatctgctg gcgtttcttg acgacgaact gacgccaaat
    12181 aatcaggaag agcaaaaacg ttgtgcgaag ctgaagggcg atctcgacac ttataaatgg
    12241 gatggtctgc gcgatcatac ggatatcgct atagatgatg atctctggcg tcgtttgagt
    12301 accgacaaag ccagctgcct caaccgtaac tgttactact atcgtgaatg cccgtttttt
    12361 gttactcgtc gggagattca ggaagcggaa gtggtggtgg caaaccatgc gctggtgatg
    12421 gcggcgatgg aaagcgaagc cgtactgcct gacccgaaaa atctgctgct ggtgctggac
    12481 gaaggtcatc acctgccaga tgtggcccgg gatgcgctgg agatgagcgc tgaaatcacc
    12541 gcgccctggt atcgactaca gctggacttg ttcacgaaac tggtcgctac ctgcatggag
    12601 cagtttcgcc cgaagaccat cccaccgctg gcgatccctg aacgtttgaa tgcgcattgt
    12661 gaagagttgt atgagcttat cgcctcgtta aacaacattc tcaatctcta catgcctgcc
    12721 gggcaggagg cagagcaccg ttttgcgatg ggcgaactgc ctgatgaatt gctggagatc
    12781 tgccagcggc tggcaaaact caccgagatg ctgcgtggtc tggcggagtt atttcttaac
    12841 gatttaagtg agaaaaccgg cagccacgat attgtgcgtc tgcatcggtt gattttacag
    12901 atgaaccgcg cgctggggat gtttgaggtg caaagcaaac tctggcggct ggcgtctctg
    12961 gcgcaatctt ccggtgcgcc ggtgaccaaa tgggcgacgc gggaagagcg cgaagggcag
    13021 ctacacctct ggtttcactg cgtgggaata cgtgtcagcg atcagctgga aaggctgctg
    13081 tggcgcagta ttccgcacat tattgtcacc tccgcaacct tgcgttcgct gaatagtttt
    13141 tcgcgtttgc aggagatgag tggtctgaaa gagaaagcgg gcgaccgttt tgtggcgctg
    13201 gattccccct ttaaccactg cgaacagggc aaaattgtta ttccccggat gcgctttgag
    13261 ccttccatcg acaacgaaga gcaacacatt gccgaaatgg cggccttttt ccgtgaacag
    13321 gtggagagca aaaaacatct cggtatgttg gtactgtttg ccagcggacg ggcgatgcag
    13381 cgctttctcg actatgtgac ggatttacgc ctgatgttgc tggtgcaggg cgatcagccg
    13441 cgttatcgct tagttgaact gcaccgcaaa cgcgttgcca acggtgagcg tagcgtgctg
    13501 gtgggcttac agtcatttgc cgaagggctt gatttgaaag gtgatctgct cagccaggtg
    13561 catatccaca aaatcgcttt tccgcccatc gacagcccgg tggtgatcac cgaaggggaa
    13621 tggctgaaaa gcctcaaccg ctatccgttt gaggtgcaaa gcctgccgag cgcctcgttt
    13681 aacctgattc agcaggttgg gcgactgatt cgaagccacg gttgctgggg cgaagtggtt
    13741 atctacgaca aacgcttgct gacaaaaaat tatggcaagc gactactgga tgcattaccg
    13801 gtatttccga tagagcaacc ggaagtccct gaaggtatag ttaaaaagaa agaaaaaacg
    13861 aaatccccac gccgtcggcg gcgttaatga tgtgagtcag gtaaggagtc gtaaatggac
    13921 tatcgcaaaa taattaaaga gatcgggcgc gggaaaaacc acgcgcgcga tttagaccgg
    13981 gatactgcgc gcggtctgta tgctcatatg ctcaatggtg aagtccctga ccttgagttg
    14041 ggcggcgtat tgattgcgct gcgtatcaaa ggggaagggg aagcagagat gctcggcttt
    14101 tacgaagcca tgcaaaatca caccatcaag ctgacgccgc ctgcaggcaa gccgatgccg
    14161 attgtcatcc ccagttacaa cggcgcgcgt aaacaggcta acctgacgcc attattggcg
    14221 attctgctgc ataaactcgg ttttcctgtg gtggttcacg gagttagcga agatccaact
    14281 cgcgtgctga cagaaaccat tttcgaactg atgggtatta caccaacgtt gcacggcggg
    14341 caggcgcagg cgaagctcga cgaacatcaa ccggtgttta tgcctgtcgg cgcgttttgc
    14401 ccgccgctgg aaaaacaact ggcgatgcgc tggcggatgg gcgtgcgtaa cagtgcacat
    14461 accctggcga aactggcgac gccatttgcc gaaggtgagg cactgtgtct ttccagcgtt
    14521 tcgcatccgg aatacattgg acgcgtcgcg aagttcttta gcgatatcgg cgggtgggcg
    14581 ttactaatgc atggtactga aggcgaagtg tatgctaatc cgcagcgctg cccgcagatc
    14641 aatctcattg accgtgaagg gatgcgggtg ctgtatgaaa aacaggaccc tgctggtagc
    14701 gagttactgc cacaagcaaa agatccggaa accacggcgc agtggactga gcgttgcctg
    14761 gctggcagcg aagcgattcc cgaatcgctg aaaatccaga tggcttgctg cctggtggct
    14821 acgggtgaag tggcaactat cagcgatggc ctggcgcgcg ttaatcaggc attttaattt
    14881 ttatctcagc cggatgagcc acgtcatccg gcttttaccc caccgcaata tgaaattcct
    14941 gcatctttat tgaccttccc gcgtccggcg tgcagcataa aaaaacaaca aacacataac
    15001 ataaacagga gttaaccatg gaaagtggtc atcgctttga tgctcagacg ctgcacagtt
    15061 ttattcaggc tgtatttcgt cagatgggta gcgaggaaca agaagcgaaa ttagttgccg
    15121 atcatttaat cgcggcaaac ctggcagggc atgattctca tggtattggc atgatcccaa
    15181 gctatgtgcg ttcctggagt caggggcatc tgcaaattaa ccatcatgcc aaaatcgtta
    15241 aagaggcggg ggcggcagtc acgctcgatg gcgatcgcgc attcggtcag gtcgcggcac
    15301 acgaagcgat ggcgctgggg attgagaaag cgcatcagca cggtattgct gccgtggcgc
    15361 tacataactc gcatcatatc ggccgtatcg gttactgggc ggagcagtgt tcagcggcgg
    15421 ggtttgtctc tatccacttt gttagcgtgg tcggtattcc aatggtcgcg ccgttccacg
    15481 gtcgcgacag ccgctttggc accaatccct tctgtgtggt tttccctcgt aaagataatt
    15541 tcccgctgtt gcttgattac gccaccagcg ccattgcatt tggcaaaacc cgcgtcgcct
    15601 ggcataaagg cgtccccgtg ccgccaggtt gcctgattga cgttaacggc gtgccgacga
    15661 ccaatccggc ggtaatgcag gagtcgccgt tggggtcgct gttgaccttt gccgaacata
    15721 aaggctacgc ccttgcagcg atgtgtgaaa ttcttggcgg ggcgctttct ggcggtaaaa
    15781 cgacgcatca ggaaacgttg caaaccagcc ccgatgccat tcttaactgc atgaccacta
    15841 tcatcatcaa cccggaactg ttcggcgcgc cggattgtaa cgcgcagacc gaagcctttg
    15901 ccgagtgggt gaaagcctcg ccgcatgatg acgataagcc gattttgcta ccgggcgagt
    15961 gggaagtgaa cacgcgtcgc gaacggcagg agcaggggat tccattggat gcgggaagct
    16021 ggcaggccat ttgtgatgcg gcgcggcaga ttggtatgtc ggaagagacg ttgcaggctt
    16081 tctgtcagca gttagccagc taaaaaaaag cccgtccagt ggcggacggg caaacaaggg
    16141 taacatagga tcaatgaggg ttagagcata tgcgtctgtc ggcaaacaga cagggaaata
    16201 cttgtgctgg acgtagcgta aacgcctgat ccgtccaacg gttcggattt tgtaggcctg
    16261 ataagacgcg gtagcgtcgc atcaggcatc tgttgccgga tgcggcataa acgccttatc
    16321 cgtcctacga attacttata aattaccgca gtaccgctta atttattgtt gttggtggcg
    16381 gaagtgatgc tgtatccact ggcaccggct gccgccgctt tctcagccag tttcgcttcc
    16441 agggcatcga gggtggatgc gccatcggca gaaaccacgc cgattttatt catgttctgt
    16501 gcctgggatg ccgttaccgg ttccgccgcg aacacgccaa atgacagagt tgaaagagcc
    16561 atagcagcaa caacagtatt gatagttttc ataattaatc tctcgcaggt gatttttgta
    16621 taagtgacgt tgtttcgtcg atgtgatgag tatcacgttt ttttacgaga gataaaatcg
    16681 aagagaattg accgccttgt tcaaataaat tgattgatat ctaacataca gataaaccat
    16741 ttatttgttg tacttatgga cttgttttat gccgcgtggc agtcacagca gcgttaaagg
    16801 cacggaatgc cacgcaaaat acaaattata ctcgctatgc atctcgccag ttgtagttaa
    16861 cgtaactggc tgtctttcga acctcagcga ttatatcctg tatacgctgg tttttgtaaa
    16921 tctttctcct gctgacaatg aatacataag cgcacgccag gaatggcttc ccgacgggct
    16981 tgcgggatgg gggcaccgca ctcttcacat tcatacaggc tttcgccacg cggaatttca
    17041 ccccgagcgc gggcaatcgc atcttcaatt gtactgttga tctgttcgtt gacggcgtca
    17101 tcgttagccc aagcggatgc catatcgtcc tccccatatc aatgcttgta cagttaagtg
    17161 tagctaatat agggacgaac ctgggcagtt caagcgtcag atctccgacc attcccgcag
    17221 cagattatga taaagattga gcagcgacag gatctcttca ttttcaccat agcggctttt
    17281 cagcgactga atatttttgt ccagttcaaa cagcatggcg cgctttttat catcgcggat
    17341 catcgactgg atccacataa atgatgccac tcgtacgccg cgggttacgg gtgtcacgca
    17401 atgcaggctg ctggagggat acaacaccag atcacctgcc gggagtttta cccgatgttg
    17461 tccaaaggtg tcattaacga ccagttcgcc gccgtcgtag ctttgtggat cgcttaaaaa
    17521 cagcgtggca gaaaggtcag tgcgcatcca gccgttttgt ggatggctgc gtactgcgcc
    17581 atccacatgg aaaccatagg tttcattgtt ctgatagcga ttaaacagcg gcgtggaaag
    17641 ggtacgcggc aaggcagcgg caaagaacaa tgcatgttgg ttaaccgcgt tcagcacctc
    17701 attttgcagt gcggcgtata acgcgctgcg ggtgtctact tgctgattgt tcttaacttg
    17761 tgcaccttgt gctccggtgg tgacgcgtcc atccacccat tcggcttgtt ccagttgttc
    17821 gcgaaaatgg gcgacgtcct gtggcgataa cacgccggga atgtggtaca tcatacaagt
    17881 tctccaaaaa gtggggcctg cgccccacat ctgaatcaga aatgcatatt ggctgtgagc
    17941 aagaaggttc ttggctcgcc cgggtgataa cggtagccgc tcttgttgat tgaggcgacg
    18001 taatcggtat caaacaggtt gtaaacgttt agctggaagt cgagattgcg attaacccga
    18061 taccccagtt tggcatctgc gacccagtaa ccttctgtaa atgctggcgt tcccaccgcg
    18121 ccgtctgaac ctttatgtat actgccgata tagcgtgcgc ccgcgccaac agagatatca
    18181 tcggttgcct gatattggct ccataaggtg aaggcgtgct ccggggtata cggcagcgat
    18241 gaggaaccat cctgggcaac atctttgccg tttttgatgg ttgctttttg ctgggtatag
    18301 ccgccaatca cctgccacgc gggagtgata ttccctgcca cggatatctc atagccttcg
    18361 acgcgtttct taccgtattg cgagtaagtc ccgtcatcat tttgctcaac ttcattttcg
    18421 atatcagtac ggaacagcgc ggcggtgagc aacaggcgtt tatccagaac ctgccatttg
    18481 gtgccaatct cgctggtgtt ggctttttgc ggtttaaaat cggtgcggtt ggcactgtta
    18541 ccactgccag actgcgcaag ggcgaagttg ttgccgcccg gaggctgctg ggaaacggca
    18601 tagttaatat agacattgcc gttttccgtc aggtgataca gcgccccggc tttccagttc
    18661 accagattgc ccgacttggc ggtgtcgacg gtggtgactg gagaaccttt tgccacacca
    18721 gctgggcagg tgatggcacc gcgtccgctg ccgccgcagg tggtggcact gtcatattca
    18781 gtatgataat tatccagacg gatcccgccg ttcagctcaa aatcacgggt gatttgcagc
    18841 gtatcgaagg cgtaaattgc gaaggtatcc gtctgaccat tggcgtttgc gccgttgcgc
    18901 gtcaggccgc cgggatgaat tctgctgtca ggatgataaa tatttaccgc cggtaacgtc
    18961 accggattaa cgccatagtt agtctgcgtt tcacgggtaa attccacgcc ggtactgaca
    19021 tcatgaccga tagaaccggt atagaacgtc gaggtcaggt tggtctggtt ggtgagaatt
    19081 ttattactca catctttgat attcgccgtg cgtgactagg tccagctatt cacatcgctg
    19141 gtgggctgag taatattcga cgccccgccc ataatcgccg tcatcaggta atcctgcttt
    19201 acgcgcgacc aacgggtagt attgcgaatg gtggtgttat cgttgatgtc gtgctcaaaa
    19261 cgcatggtgg cggtgtcggt ggtcgaatcg tcgtaatcgg aatccgtgcc gtaaaagtta
    19321 tgagtatcaa cttttccgga atgattcagg gccgccgctc ccgcagatgg ggcagaatag
    19381 cccggcaaac cgatggtcgg aatgccgccg tctggcgtgt tgtgctgagt gacatgcaga
    19441 taattaagat acaaacgatt cgctgtacca aggccaaaag cgacagaagg ggcgacgccg
    19501 taacgctcat ttttgacttt gtcgcgtccg gcatcgtggg ttttttcgcc catcacattc
    19561 aggcgcaccg cggtagtatc accaatgacc tgattgacgt ccagcgtgcc gcggcggaac
    19621 caggcgctgc caatactggc ggaggcgtca atgccggaat cattgcgcgg ctgcttgctg
    19681 atcatattga tcgagcctgt cggtgcgctg cgcccgtagt cggtgccgga cggcccttta
    19741 atcacttcga cctgctcggt attgaaggtg tcgcgcgaga cgctgccgat atcgcgaata
    19801 ccatcaatat aaatactgtt agaggtatcg gcaccacgca tataaatggc gtcgccagtg
    19861 gtggagttac cgttctcacc cgcaaaaaac gcacccacgc
//
LOCUS       DADMEQ010000070        19704 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.070, whole genome shotgun sequence.
ACCESSION   DADMEQ010000070 DADMEQ010000000
VERSION     DADMEQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.070"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            205..1302
                     /gene="mltA"
                     /locus_tag="JNZ05_002505"
     CDS             205..1302
                     /gene="mltA"
                     /locus_tag="JNZ05_002505"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HAY8565699.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGKTHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1541..2347
                     /gene="tcdA"
                     /locus_tag="JNZ05_002506"
     CDS             1541..2347
                     /gene="tcdA"
                     /locus_tag="JNZ05_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HAY8565700.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2398..2841)
                     /gene="csdE"
                     /locus_tag="JNZ05_002507"
     CDS             complement(2398..2841)
                     /gene="csdE"
                     /locus_tag="JNZ05_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HAY8565701.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2841..4046)
                     /gene="csdA"
                     /locus_tag="JNZ05_002508"
     CDS             complement(2841..4046)
                     /gene="csdA"
                     /locus_tag="JNZ05_002508"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HAY8565702.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPALARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4238..4465
                     /locus_tag="JNZ05_002509"
     CDS             4238..4465
                     /locus_tag="JNZ05_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HAY8565703.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            4824..5741
                     /locus_tag="JNZ05_002510"
     CDS             4824..5741
                     /locus_tag="JNZ05_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HAY8565704.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKIPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5760..6155
                     /locus_tag="JNZ05_002511"
     CDS             5760..6155
                     /locus_tag="JNZ05_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HAY8565705.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6148..7248
                     /gene="rlmM"
                     /locus_tag="JNZ05_002512"
     CDS             6148..7248
                     /gene="rlmM"
                     /locus_tag="JNZ05_002512"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HAY8565706.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7292..8023)
                     /gene="fucR"
                     /locus_tag="JNZ05_002513"
     CDS             complement(7292..8023)
                     /gene="fucR"
                     /locus_tag="JNZ05_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HAY8565707.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGAPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGYLDEVTHIISDERQVATSLVTA"
     gene            complement(8081..8503)
                     /gene="fucU"
                     /locus_tag="JNZ05_002514"
     CDS             complement(8081..8503)
                     /gene="fucU"
                     /locus_tag="JNZ05_002514"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HAY8565708.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8505..9924)
                     /gene="fucK"
                     /locus_tag="JNZ05_002515"
                     /pseudo
     CDS             complement(8505..9924)
                     /gene="fucK"
                     /locus_tag="JNZ05_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(10108..11808)
                     /gene="fucI"
                     /locus_tag="JNZ05_002516"
     CDS             complement(10108..11808)
                     /gene="fucI"
                     /locus_tag="JNZ05_002516"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HAY8565709.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHTCGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     "
     gene            complement(11841..13157)
                     /gene="fucP"
                     /locus_tag="JNZ05_002517"
     CDS             complement(11841..13157)
                     /gene="fucP"
                     /locus_tag="JNZ05_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HAY8565710.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRTYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRITGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13704..14351
                     /gene="fucA"
                     /locus_tag="JNZ05_002518"
     CDS             13704..14351
                     /gene="fucA"
                     /locus_tag="JNZ05_002518"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HAY8565711.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14379..15527
                     /gene="fucO"
                     /locus_tag="JNZ05_002519"
     CDS             14379..15527
                     /gene="fucO"
                     /locus_tag="JNZ05_002519"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAY8565712.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15580..16425)
                     /gene="xni"
                     /locus_tag="JNZ05_002520"
     CDS             complement(15580..16425)
                     /gene="xni"
                     /locus_tag="JNZ05_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HAY8565713.1"
                     /translation="MRSLFLFSQPAIACSGIECYPYRLIFKGVIVAVHLLIVDALNLI
                     RRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRSSGWRHQRLPDYKADRPP
                     MPEELHDEMPALRAAFEQRGVPCWSASGNEADDLAATLAVKVTLAGHQATIVSTDKGY
                     CQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVPGVAGIGPK
                     SATQLLVEFQSLEGIYENLDAVAEKWRKKLETHKEMAFLCRDIARLQTDLHIDGNLQQ
                     LRLVR"
     gene            complement(16446..17813)
                     /gene="sdaB"
                     /locus_tag="JNZ05_002521"
     CDS             complement(16446..17813)
                     /gene="sdaB"
                     /locus_tag="JNZ05_002521"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HAY8565714.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDISIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17871..19160)
                     /locus_tag="JNZ05_002522"
     CDS             complement(17871..19160)
                     /locus_tag="JNZ05_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY8565715.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLSMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4870 a         4825 c         5197 g         4812 t
ORIGIN      
        1 gtggatgcgc actatactcc gctcgcgttt tcatgcaacc ttttttttac cccgatcacg
       61 gatttagcat gattttgaac aaaaacaaac cactaagctt attttttgtg caaaatttgc
      121 gtcagggaga ggctgctcat ttcattaaac gcggcggttt gttatcttcg ttgcgcctta
      181 ttttttaacc tgaagaagag aacaatgaaa ggacgttggg taaagtacct tcttatgggc
      241 acggttgtgg caatgcttgc cgcctgctct tccaaaccaa ccgatcgcgg acagcaatat
      301 aaagacggga aatttaccca gcctttctct ctggtgaacc agccagatgc cgttggcgcg
      361 ccgattaacg ccggtgattt tgccgagcaa attaaccata tccgtaattc gtcaccgcgt
      421 ctgtatggca accagagtaa tgtttataac gcggtgcaag agtggctgcg cgcaggcggt
      481 gatacccgca atatgcgcca gttcggcatt gatgcctggc agatggaagg tgccgacaac
      541 tatggtaacg tgcagtttac aggctattac acgccggtaa ttcaggcgcg ccatacccgc
      601 cagggcgagt tccagtatcc tatttaccgt atgccgccaa aacgtggtcg tctgccgtct
      661 cgtgcggaga tctacgccgg ggcattgggc gataaataca tcctcgctta cagcaactct
      721 ctgatggata acttcattat ggatgtgcag ggtagtgggt atatcgactt tggtgatggc
      781 agtccgctta actttttcag ctatgcaggg aaaaacggtc atgcctatcg cagcattggt
      841 aaggtgctga tcgaccgtgg cgaagtgaaa aaagaagata tgtcgatgca ggcgattcgt
      901 cattggggca aaacacacag tgaagccgag gttcgcgagc tgctggaaca gaacccgtct
      961 ttcgtcttct ttaaaccgca atcttttgca ccggtgaaag gggcaagtgc ggtgccgctg
     1021 gttggccgtg cttctgttgc ctccgatcgt tcaattattc cgccaggtac taccttgctg
     1081 gcagaagtgc cgctgctgga taataacggc aaatttaacg gtcagtacga attgcgtctg
     1141 atggtggcgt tggatgtcgg gggcgcaatc aaaggccaac acttcgatat ctatcaaggg
     1201 atcgggccgg aagccggaca ccgcgcaggt tggtacaacc actatggacg ggtttgggtg
     1261 ctgaaaacgg ctccgggcgc aggtaacgtc tttagcggct gatgtgatat tctgtatgcc
     1321 gtttttacgg atatgacagg gtgaggggta acctcacatc ctgattattg actaagtgcg
     1381 cgttgttcat accggatgcg gcgtgaacgc cttatccggc ctacaaatca tgcaaatcca
     1441 aaatattgca ggagttatgt aggcctgata agcgtagcgc atcaggcatt tatgcgttta
     1501 gggtgaggga taacctcgcc ctttttaatt ctgaggtgct atgtctgtgg taattagtga
     1561 tgcatggcgt cagcgttttg gtggcacagc gcgtctgtat ggtgaaaaag cgttgcaact
     1621 gtttgctgac gcgcatattt gtgtggttgg tatcggtggt gtcggttcct gggcggcgga
     1681 agcgctggcg cgcacgggga ttggcgcaat cacgcttatc gatatggatg atgtgtgcgt
     1741 caccaatacc aatcggcaaa ttcacgctct gcgcgataac gtcgggctgg caaaagcgga
     1801 agtcatggcg gagcgcattc gccagattaa cccggagtgc cgtgtaacgg tggtggatga
     1861 tttcgtgacg ccggataacg tagcgcaata tatgagtgcc ggttattcgt acgtgattga
     1921 tgccattgat agtgtacggc ccaaagcggc gctgattgct tattgtcggc gcaataaaat
     1981 cccgctggtc acgaccggtg gcgcgggtgg gcagattgac ccgacgcaga ttcaggttac
     2041 cgatctggcg aaaacgattc aggatccgct ggcggcgaag ttgcgcgagc gcctgaaaag
     2101 cgattttggc gtagtgaaaa acagtaaagg taagctcggc gtggattgcg tgttttctac
     2161 tgaagcgctg gtgtacccgc agtcagacgg tacggtgtgt gcgatgaaag ccacggcaga
     2221 agggccgaag cggatggatt gtgcatctgg atttggcgcg gcaacgatgg tgaccgccac
     2281 ctttgggttt gttgcggttt ctcatgcgct gaagaagatg atggcgaaag cggcgcgtca
     2341 gggttaaaca atcaggtcgg atgagacgcg aacgtcttat ccgacccggt tctgtgctta
     2401 aacctgcttc gtcgcagcga taatcgcctc gcttaacgca tttaacccct ggctgcgtga
     2461 ggcgctaagc tgcgcacgta atcccagctc atcaaacaat gccagcggcg actgtgcctg
     2521 taattcggcg gcggttttcc cctcaacggc agtcaacaac accgccaaca ggccgcgcac
     2581 aatgcgccct tcgctgtcgc caaagaagtg cattttgccg ttttcagcca cggtatatcc
     2641 cagccagacg cggttttcgc atccggcaat ctctttagcc tgcgctttta actcgtctgg
     2701 caaagccgga agctgtttcc ccagcatgat caactggcga tatttatctt cccattgcga
     2761 caacggtgcg aaggtattgc gtaacgtttc tgcggttacg gttgtgccga acggatgtcc
     2821 ggcgaattgc gggtttgtca ttaatccacc aataattcca gcgcgcggtc aacggcattc
     2881 accagcgcat ccacatcact ctttgtatta tatggcgcaa atgaggcgcg cagtgtgccg
     2941 gttacgccta attctgccag tagcggctga gcgcaatgtt gcccggcccg caaggcaata
     3001 ccgtactccg ccagtagcgt caccatatcg ctgtgatgaa cgccagcaaa atcaaaggcc
     3061 agcaggctgg aatcctggca gcggaatgaa cgaaagcctg gacgtttcgc cagcgcttct
     3121 tctgcaagcg ttgctaagct acggctccag ctttcggcct ggttgatatc gtaatctgcc
     3181 agccattcca gcgccgcgct taatcctatg acgccagcga catttggcgt tcctgcttcc
     3241 agtttccacg gcgcagattg agtggtgaaa ccgtcaaaac tcacttcgtg aatcattttg
     3301 ccgccaccca gccagggcga catcgcttcc agcagttctg atttgccata cagcacgccg
     3361 atacccgtcg ggccatacag tttgtgacca gagaaagcat agaaatcgat atccagttgc
     3421 tgaacatccg cggggaaatg caccgccccc tgagcaccat caaccatcac caccatcccg
     3481 gctgaatgag caaaggtaat cgctcgcgcc agagccgggc aaccgccagt cacgttcgac
     3541 atctgaccca acgccagaat ccgactacgg ggagtaatca gttctggcaa caaatcgaca
     3601 tccggcagtc gctgcgcatt aagcggcaat ttcaccactt tggctctagt ttgttgggcg
     3661 accatcagcc aggggacgag gttagcgtgg tgttctgcca cgctgacaat aatctcatcg
     3721 cccggttgca gacgcggacg cgcatagcat tgtgccacca tgttgatgga ttcagtggtg
     3781 ccgcgcgtcc agacgatagt tttatcatcc ggtgcattca gtaattgcgc cactttctct
     3841 cgtgcagctt cataacgcgc ggtcaggcgt tgggcttcgg caaactggct gcgatggacg
     3901 tttccggcgc tcaggctgta aaactgtcga gtggcttcaa ccacggcttc aggtttaagc
     3961 gcggtcgcgg cgctgtcgag atagacgccc gcatcctgta gtgcgggaaa ctgggcgcga
     4021 aactgcgcgg gattaaaaac gttcatggta ctcctcggct tgatgcaccg atcgtgacgc
     4081 aaaattcact ttgatacaag gagagtgcta accatcagaa ttatctggat atcctggcga
     4141 agccgcgtaa gtaacatatg ctgaatagtg ttaggcgtta attttagcct gttaatcaat
     4201 actattaaga tttattaatc gctttaagga gaagatgatg aaaaagactg ccgcaattat
     4261 ttctgcctgt atgctgactt ttgccctgag cgcctgttcc ggttcgaact atgtgatgca
     4321 caccaatgac ggacgtacca tcgtctctga cggcaaacca cagactgata acgataccgg
     4381 tatgatttcg tataaagacg ctaatggcaa caaacagcag atcaaccgta ctgacgtgaa
     4441 agagatggtc gaactggaac agtaattcgc gatcgcaagg taaaaaaaag caccgcaatt
     4501 aggcggtgct acattaatca ctatggacag acagggtaaa tgtacaggaa gtgaaaaaag
     4561 gtagctttgc taccatggtc tgaatcgcag accaattgca aacacaacaa cacaacaaca
     4621 caacatcaca accgtaagcc aaaagttcac cagaatacgc attccgataa aacttttcgt
     4681 tccggctcag gaagtgccgc cactataggt atttgctggt ataagctcaa cggacaattt
     4741 ataatggctc agattaaaaa aactaatagg ttacacagtg tgatctaatt gttaaattca
     4801 tttaacatca aagtttaaaa gccatgtcta aacgattacc accgctaaat gccttacgag
     4861 tttttgatgc cgcagcacgc catttaagtt ttactcgcgc agcagaagag ctttttgtga
     4921 cccaggccgc agtaagtcat caaatcaagt ctcttgagga ttttttgggg ctaaaactgt
     4981 tccgccgccg taatcgttca ctcctgctga ccgaggaagg gcaaagctat ttcctcgata
     5041 tcaaagagat attttcgcaa ttaaccgaag cgacgcgtaa actccaggcc cgtagcgcca
     5101 agggggcgtt gacggtcagt ttactcccca gtttcgccat tcattggttg gttccgcgac
     5161 tttccagctt taattcagct tatccgggaa ttgacgttcg aatccaggcg gttgatcgtc
     5221 aggaagataa gctggcggat gatgttgatg tggcgatatt ttatggtcgg ggcaactggc
     5281 cggggctacg ggtggaaaaa ctgtacgccg aatatttatt gccggtgtgt tcgccgctac
     5341 tgctgacagg cgaaaaaccc ttgaagatcc cggaagatct ggctaaacat acgttattac
     5401 atgatgcgtc acgccgtgac tggcagacat atacccgaca gttggggtta aatcatatca
     5461 acgttcagca agggccaatt tttagtcata gcgccatggt gctgcaagcg gctattcacg
     5521 ggcagggagt ggcgctggca aataacgtga tggcgcaatc tgaaatcgag gccggacgtc
     5581 ttgtttgccc gtttaatgat gttctggtca gtaaaaatgc tttttatctg gtttgtcatg
     5641 acagccaggc agaactgggt aaaatagccg cctttcgcca atggatcctg gcgaaagccg
     5701 ctgctgaaca agaaaaattc cgctttcgtt atgaacaata atttacgtag ggtacgacca
     5761 tgaccagccg ttttatgctg attttcgccg ccattagcgg cttcattttt gtggcactgg
     5821 gcgcttttgg tgcgcatgtg ttaagtaaaa ccatgggggc cgttgagatg ggctggatcc
     5881 aaaccggtct cgaataccag gcgtttcata cgctggcgat cttaggtctg gcggtggcaa
     5941 tgcagcgtcg catcagtatc tggttttact ggagtagcgt tttcctcgcg ttaggcacgg
     6001 tgctgttcag cggcagcctt tattgcctgg cgctgtccca tctgcgtttg tgggcgtttg
     6061 tcacaccggt tggcggcgtg agcttccttg cgggctgggc gttaatgtta gttggtgcta
     6121 tccgtttaaa gcgcaagggc gtaagtcatg aataaggttg tattgctgtg ccgtccgggc
     6181 tttgaaaaag agtgcgccgc agaaattacc gataaagccg gccagcggga aattttcggt
     6241 tttgcccgcg tgaaagagaa tgcgggttat gtcatttatg aatgttatca acctgatgat
     6301 ggcgataagt taatccgtga gctgccgttc agttcattaa tttttgcccg ccagtggttt
     6361 gtggtggggg aactcctgca gcatttgccg ccagaagatc gtattacccc cattgtcggc
     6421 atgttacagg gtgtagtaga gaagggcggt gaactgcgtg ttgaagttgc cgataccaac
     6481 gaaagcaaag agttgctgaa attctgccgt aagttcactg ttccgctacg cgctgccttg
     6541 cgcgatgctg gggtgctggc gaactatgaa acgccgaagc gtccggttgt gcatgtattc
     6601 tttattgcac caggatgctg ctataccggt tactcataca gcaacaataa ttcgccgttc
     6661 tatatgggca ttccgcgcct gaaatttccg gcagaggcgc cgagtcgttc cacgctcaaa
     6721 ctggaagagg catttcatgt gtttattcct gcggatgagt gggatgaacg cctggcgaac
     6781 gggatgtggg cggtggattt aggcgcttgc cctggcggct ggacctacca actggtgaag
     6841 cgcaacatgt gggtttattc cgtcgacaac ggcccgatgg cgcaaagtct gatggatacc
     6901 gggcaggtga cgtggctgcg ggaagacggt ttcaaattcc gtccgacacg cagcaatatc
     6961 tcctggatgg tatgcgatat ggttgaaaaa ccggctaaag ttgcggcatt gatggcgcag
     7021 tggctggtta atggctggtg ccgtgaaacc attttcaatc tcaaactgcc gatgaaaaaa
     7081 cgctacgaag aagtatcaca caatctggcg tatattcagg cacagcttga tgaacatggc
     7141 ataaatgctc agattcaggc gcggcagttg tatcacgatc gcgaagaagt gacggtgcac
     7201 gtccgccgca tctgggctgc ggtgggtggt cgtcgcgacg agcgataaca ggcaaggccg
     7261 gggcgtcgtc tccggcctgc taccctttcc gtcaggctgt taccaaagaa gttgcaacct
     7321 ggcgctcatc agaaataatg tgcgttacct catccagata cccgatgcgg gcttcccctg
     7381 aacgattaaa tttactttta tcaatcaata acaacgattg cgcggcacgt tttaatagca
     7441 tcgatttgta atcagcgttg atcgcattgg agtcccacag tgcgccgctg ctatcgatcc
     7501 cttcacaaga aaaaataaac agatcgattt ccagcgattt aagttgggaa atcagcgagg
     7561 gattgacgta acagccatat ttgcgctcaa gtgtgccgcc ggaactgatc agttgaatgc
     7621 gttcgcgttt accgagttca tggcaaatcg gatggctatt ggtgaagacc tgaatgttga
     7681 tgtcaggcaa ctggcgtgcc agataccagc aagttgaact ggcgtctaag gctatcacca
     7741 tcccttcttc aatccacgcg agcgcctcgc gcgcgatatc tgctttatgc gcataatggc
     7801 ttttcagcct gatgtgaaag ggagcgccac tgtcttgatt ttgacggtgg atatatttag
     7861 cgcgtccatg attgcgcaga attttaccct gcgtctgtaa ttcattgaga tcgcgacgaa
     7921 tggtttcttt actgaccttt agctgttcag agagagcttc cgtggtcagg ctggtatggt
     7981 tcagcagcag gtcgactatc gcttgctggc gtgccgcttt catatttttc accccatcat
     8041 tcgataaccg cccgcgtcat gcgggcgtac cggtatgaga ttacggtgtt accccttttt
     8101 ttaaaagaat attcccgtac ttcgctcgtt cgcctgtgat aacgatcgca aaggcttttt
     8161 gcgcccgttc ataaaacgca aaacgattga tgcggatgat gtcaggacac ggggcttgta
     8221 gtgaaagcgc attacggtaa cgtcgttcta cttcaggatc gagagtgtca ccctctaccg
     8281 ccgccatcat caccagcggc ggggcataac tgtccagctc aaataacggg ataatcgcct
     8341 ggagcaagtc gctcaccaac aggccatcag cgcggatcac ctgcggtccc atcgaatggg
     8401 cgggaaagtg agcatcggaa aaaataattt catctccatg tcccatctct gccagcactt
     8461 tcaatagttc gggagaaatt aacggcgaaa ttgttttcag catttcacac ttcctctata
     8521 aattcaggtt cagtttgcgg gtagaaataa cggtactgat aatgaatctg tgcacgggct
     8581 tcttccgggc tgttaaattc ccctacgcca taccaaccga acagcgcagc tcctgcgacg
     8641 gtcgtttcgg catcgtcgag aacttttacc ggaatatcaa gcatattggc tttaatctga
     8701 ttccacaatg tgttgcgact tcctccaccg actaacaata attcataggc tttaaagtgc
     8761 ccgatttttt ccagcatctg tagattgcgc tgtaattgcg cagttaaccc ttccagcgcc
     8821 gcgcgataga aatgcccccg cgtggtatta agcgtcactc cttgccagcc agcgttctga
     8881 cacgacaata aatcacactg cattttcaca ccatccgcgc caggtgcgat cagacgagct
     8941 tcttcaatca acatttgcca gggtgtttca gccgtccaga acagttttct cacccattcc
     9001 agcacgccgg atgccagcca ttgcatacct gggttataca accatgcctg gctatctagt
     9061 tcgcaggtgg aaccggcgta ctgacttaac agcgaagtat caacctaggc gctgcgaacc
     9121 attaaaattt cccatgtacc ggaggagagc acgggttcat tttgttcggc accagcgcca
     9181 aaaagggcga actgggtatc gtgacctgcg gaaatcaccg gtatgccaac gggtaagccg
     9241 agcattgctg cggcgctgtt ctgtagcgta ccaatctgtt cacccgcttc caccagacga
     9301 gggaagagtc ggcgtggaat accggtggct tgtaaaattt gcggactgaa atcgcgttgc
     9361 tggatatcca gcatctggct ggttccggct atcgtgatat cagtagtgaa ttcgccggtt
     9421 aaacggtggt taatcagcga cgaaatgaag agccaggcgt gcgcgcgttc cagcagttgt
     9481 ggatgatttt ctttcaacca caccaactta tataacgtat tgaaactaaa ggctccgacg
     9541 ccagaaatag cctgcaaccg ctgtgcggag attaaccgtt caatattgtc cataaccgct
     9601 gctgttcgcg gacatttcca gctaatgatc ggatagagca gattgccttg cttatctacc
     9661 agagcgccat ccacaccaaa ggtggtgacg gcgataccgc ggatgtggca ttcagtcagt
     9721 tcactattga tttgccgaca gcaatcagca aagcgttgca aaatggcagt ctaaagacca
     9781 ctggtgccag gtgttgtttt ccatcgcgat atcgctggca ttaggcgttg aggcgcgggc
     9841 aacaattttt ccctgccgat taaccgcgat ggccctgaca ttggtcgcgc cacagtcgag
     9901 taccaggata acttcttgtt tcataatcgc tcctgcaata tagccggata acattgttta
     9961 tccggctaac cactcttgct tgttcaggcc cggtagccgg agcgaccggg catcacatca
    10021 gggtgtaatg tattaacgct tgtacaacgg accgtagttc tggcaagcgc ggtaatcctg
    10081 gccttcaata tccatgccgt gcgcagccta ggcagaagga cgatacactt tggtctcttc
    10141 aacgttgtgc atacataccg ggatacgcag catggaggca agggtgataa agtctgcgcc
    10201 aacgtggccg atggtcagaa ctccgtggtt ggcaccccag ttcgccatta ccgaatacac
    10261 atccgtaaac ggccctttac cagtgaggcg cggtgcaaac caggtggttg gccaggttga
    10321 gttggtgcgt ttgttgagga tgtcatgcac atccttcggc aattccacgc tccagccttc
    10381 cgcgatttgc agtaccggtc ccaggccttt gatgatgttg acacgagtca tggtgaacgg
    10441 gacgccgcct tcggtaagga agcgggaaga gtaaccgccg ccacggaagt attcgtggat
    10501 cgccgggcac cattcggtag cggcgaggca agcgtcagcc tcttgctgag agatttccca
    10561 gtgcggcttc atcgtcgggt taccttcgct gtcgcgttgt ttacaggaac cgtccagcgc
    10621 agcagaacct gagttgatca aatggatgat gccgtgttct gccagtccgt ccagtttatg
    10681 ccccgttaca cgctcaattg cttctggtga ccagtaggta cgcacatcgg caaatacctg
    10741 agcggtgccg gtgagctggt gacccattag cattgccacg ccgttaagac tgtcgttttc
    10801 ggtcgccacg acaaagggtt cgcgcacgcc attccagtca aatgaactgt tgaggatcgc
    10861 ttcggcggtg tcaccattgg gatattgatc ggtccagtga cgttgcccct ggaagcccgc
    10921 agcgatggcg ttgtagccaa gtgattcttc cacgcgacca atatcggcca gtttgctgtt
    10981 gccttgcatc atgtcgcgga tacacatcgc catcagtaaa ctttcgcgca gaactgcgcg
    11041 gctttgctcg gcattgcgtt gatactgttt gttattttca tcttcgccat agcggaagtt
    11101 tttatcagcc caggccagtg ccatttccag ttcggcttcg tcgtaaatct tctgatcgat
    11161 acggcgacgc agttcggtca tatccaccgc ctggactttc attcccagcc aggattcaaa
    11221 gaagttgtga tcaacaatag aaccggcgat acccatcgaa acgccgccca gcgacagata
    11281 gcttttacct ttcatgctgg cgacggccaa accggcgcgg gcaaagcgca gcagtttttc
    11341 ttcaacatcg gcaggaatcg atgtgtcatc ggcatcctga acgtcatgac cgtaaatgga
    11401 gaatgccggg atgcctttct ggctgtgagc tgccagagcc gctgccaggt aaacagcgcc
    11461 ggggcgttca gtgccgttaa agccccaaat ggctttcggg cgggttggat ccatgtcgat
    11521 ggtttcactg ccatagcacc agcaaggcgt taccgtaatg gtgaggccta cattctggct
    11581 gctgaatttt tcttcgcaag cagcggcttc agccataccc gcgatacagg tatcggaaat
    11641 gacacactcg acggcagctc cgcaggtatg gcgcagtttc tcggtcagca gtgcggccgt
    11701 agctttcgcc atattcattg tttgttcttc aagcgactca cgaacaccca tgcgacgacc
    11761 gtcaataacc gggcggatac caattttcgg taagctgatt tttttcatta cagattcctc
    11821 actttattcg gaaaatatgt tcagttagtt gccgtttgag aacggaaacg ggcaaagata
    11881 aagatgaccg cgaagcagag tgcggggatc agttcagcag tggggatgtt gcccgccgcg
    11941 tcactgacaa aacccatgac cggagtgaca ataccgccgc caataatggt cataacgatg
    12001 aaggacgaac cgtatttggt gtcctggccg agattcttaa tgcccagcga gaagattgtt
    12061 gggtactgaa tcgacataaa ggcgctgcat aaagtcaggg ctattaagcc cacatgaccg
    12121 ccagcgaagg ctgagatcag gcacagtgcc atagcgatta atgcgtaggc ggccaggact
    12181 ttgtgtggtg cgaagcgact gatgagccag gtaccggtga tacgaccaat aaagaagcac
    12241 accatggttc cggttaaata gttagcggca aagcctgcag tcatacctgg aatttcttct
    12301 acagcgtagc gaatcaaata gctccagcag gccgtttgtg cgccgacata gcagaattgc
    12361 gctaataccg cccagcgcca gtgacgaata cgcgccagac gagaaagcga tgcggagaac
    12421 gatccttgtt tggcgtcact gtgattatca ctctgcaatg ccgggaattt cgtcagcatg
    12481 atcagcaggg cgaccagtaa cacgatagcc acgatgatca tataaggtgt ctgtaccgat
    12541 aataccaagc tgtgtttata cgcactcaat tgctctggag acattttatc gagaacgtct
    12601 tgcgattgat gtggcacgtt agacaaaata agactttgcc caaagacaac cgcgataatt
    12661 gcgccaaacg agttaaatgt ttgcgcaaga tttaagcgga agtgactact gctttccggc
    12721 cctaataccg taacaaaagg gtttgcggca gtttccagac aacctaatcc ggctgcaata
    12781 ataaataggc caactaaaaa taaggtgtag ttcattattt ctgcagcggg ccagaataat
    12841 gcagcgccca aggcatataa aaataatccg gtaataatcc ctgctttata actgagtttt
    12901 ttcatcaata tcccagcagg gattgggata atgaaataac caaagtaaaa ggccgattgg
    12961 atcaggccag cctggaaatt tgtcagcgta aaagcctgct ggaattgagg taataaaatg
    13021 tcgttaaggt tattggctac cgcccaaaga aaaaacagtg agcacagcag cgcgaatgga
    13081 ataatgtaag ttctgctttg ccctgcatct ttatctaccg cacggtaact ctgcgtttgt
    13141 attgatgtgt ttcccatagc atcctcttag gttcagaagc ttaagcacgt aatcggttat
    13201 gttttcaaaa gtgcaaaacc agcgagttcg ggcttcatat cggtgaatat ttgcctgtct
    13261 gaaatgaact catggattca ttttcaaata aaaaacgctc tgtaatatga cggcggtcac
    13321 acttattagc tggaaggtga ctttatgtga ctaccatcac tttaataatc gaaatttcat
    13381 gcccgtttca gaaaataaat cataaaaaac acaaaccaga tgaccgtatt aattgtttgt
    13441 gccgaaaacg ggcatttctg gcgaggggaa atggaaaaat gaccgttttg ataaatattt
    13501 ttaagagcgg tcatttttaa cggggttata aaaagagaac ttgcagttat ttactgtgat
    13561 tagttgagct aggtcacaaa atataagctt gtgaatttct ttcttgttaa tcaggaagaa
    13621 gatggtgggt aattaaacgg ctaattcaat agtgtgaaag gaacaacatt attgccctgt
    13681 tttgaatcag agagaggtag ctaatggaac gaaataaact tgctcgtcag attattgaca
    13741 cttgcctgga aatgacccgc ctgggactga accaggggac agcggggaac gtcagtgtac
    13801 gttatcagga tgggatgctg attacgccta caggcattcc atatgaaaaa ctgacggagt
    13861 cgcatattgt ctttattgat ggcaacggta aacatgagga aggaaagctc ccctcaagcg
    13921 aatggcgttt ccatatggca gcctatcaaa gcagaccgga tgccaacgcg gttgttcaca
    13981 atcatgccgt tcattgcacg gcagtttcca ttcttaaccg atcgatcccc gctattcact
    14041 acatgattgc ggcggctggc ggtaattcta ttccttgcgc gccttatgcg acctttggaa
    14101 cacgcgaact ttctgaacat gttgcgctgg ctctcaaaaa tcgtaaggca actttgttac
    14161 aacatcatgg gcttatcgct tgtgaggtga atctggaaaa agcgttatgg ctggcgcatg
    14221 aagttgaagt gctggcgcaa ctttacctga cgaccctggc gattacggac ccggtgccag
    14281 tgctgagcga tgaagagatt gccgtagtgc tggagaaatt caaaacctat gggttacgga
    14341 ttgaagagta atttcgtaaa gcaacaagga gaaggatgat ggctaacaga atgattctga
    14401 acgaaacggc atggtttggt cggggtgctg ttggggcttt aaccgatgag gtgaaacgcc
    14461 gtggttatca gaaggcgctg atcgtcaccg ataaaacgct ggtgcaatgc ggcgtggtgg
    14521 cgaaagtgac cgataagatg gatgctgcag ggctggcatg ggcgatttac gacggcgtag
    14581 tgcccaaccc aacaattact gtcgtcaaag aagggctcgg tgtattccag aatagcggcg
    14641 cggattacct gatcgctatt ggtggtggtt ctccacagga tacttgtaaa gcaattggca
    14701 ttatcagcaa caacccggag tttgccgatg tgcgtagcct ggaagggctt tccccgacca
    14761 ataaacccag tgtaccgatt ctggcaatcc ccaccacagc aggtactgcg gcagaagtga
    14821 ccattaacta cgtgatcact gacgaagaaa aacggcgcaa gtttgtttgc gttgatccgc
    14881 atgatatccc gcaggtggcg tttattgatg ctgacatgat ggatggtatg cctccggcgc
    14941 tgaaagccgc gacgggtgtc gatgcgctca ctcatgctat tgaggggtat attacccgtg
    15001 gcgcgtgggc actaaccgat gcactgcaca ttaaagcgat tgaaatcatt gctggggcgc
    15061 tgcgaggatc ggttgctggt gataaggatg ccggagaaga aatggcgctc gggcagtatg
    15121 ttgcgggtat gggcttctcg aatgttgggt tagggttggt gcatggtatg gcgcatccac
    15181 tgggcgcgtt ttataacact ccacacggtg ttgcgaacgc catcctgtta ccgcatgtca
    15241 tgcgctataa cgctgacttt accggtgaga agtaccgcga tatcgcgcgc gttatgggcg
    15301 tgaaagtgga aggtatgagc ctggaagggg cgcgtaatgc cgctgttgaa gcggtgtttg
    15361 ccctcaaccg tgatgtcggt attccgccac atttgcgtga tgttggtgta cgcaaggaag
    15421 acatttcggc actggctcag gcggcactgg atgatgtttg taccggtggc aacccgcgtg
    15481 aagcaacgct tgaggatatt gtagagcttt accataccgc ctggtaaatg cgctgatgtg
    15541 ataatgccgg atacgatttg cggcgtatcc ggctcgccgt taccgtacca accgcaattg
    15601 ctgtaaattg ccgtcgatat gcaaatcggt ttgtaagcgg gcaatatcgc ggcacagaaa
    15661 cgccatctct ttatgggttt ctaatttttt gcgccacttt tcggcaaccg catccagatt
    15721 ctcatatatc ccttccagac tctgaaactc gaccagcagc tgcgtggcgc tttttggtcc
    15781 gattcccgca acacccggta cctttgaact gctgatcccc gcaagtcccc agtaatcggg
    15841 caactgctgc ggttgaacgc caaattcttt atcgataaat ggcgcatcca gccaacgttt
    15901 ctggaagtaa tcacgaatac gtaatgtcgg tgaaagtaac tgacagtagc ctttatctgt
    15961 cgaaacaatc gttgcctgat gcccggccag tgtcactttg accgccagtg tggcggctaa
    16021 gtcatcggct tcgttgccgc tggctgacca gcacgggacg ccgcgttgct caaaggcggc
    16081 gcgtaatgca ggcatctcgt cgtgcaactc ttccggcatt ggcggtcgat ccgctttgta
    16141 atctggtaaa cgctgatgac gccagccgct actgcggttt tcatcatcaa aaacggcgac
    16201 cgcgtgggtt ggctggctgt gcataatgag ctgatcgagc gcatgctggc aggtttcgac
    16261 acagggcgaa ccctgaacgg catgaatgcg acggataaga ttcagtgcat cgacaataag
    16321 caaatgaaca gccacgataa cccccttaaa gatcaggcga taagggtaac attcgatgcc
    16381 ggaacaggct atggctggtt gagaaaacag gaagaggctt cgcaaaacga ggcctctggg
    16441 agagattaat cgcaggcaac gatcttcatc gccaggccgc cgcgagaggt ttcgcggtac
    16501 ttggcgttca tgtctttacc tgtttcgtac atggtttcga taactttatc gaggcagacg
    16561 cgcggttcgc tggtacggcg cagtgccata cgcgcggcgt tcaccgcttt cactgctgca
    16621 atggcgttac gctcaatgca cggtacctga acctgccccg cgaccgggtc acaagtcaga
    16681 ccgaggttgt gctccatcgc gatttccgct gcgatgcaaa cctgcgccgg gcttgcgcct
    16741 aacagttctg ccagaccagc tgccgccata gagcacgcca cgccaacttc accctggcaa
    16801 cccacttcgg caccagaaat cgacgcgttc atcttataaa gagaaccaat ggcgctggct
    16861 accagcaggt aacgagccag tgagttagcg ttcacttcgc ggataaactt atcgtagtac
    16921 gccagcacag ccgggataat cccgcacgca ccgttagtcg gcgcagtcac cacgcgaccg
    16981 ccagcagcgt tctcttcgtt cactgccagt gcaaacatgt tgatccagtc aacaaccgcc
    17041 atcgggtcag tggtggtttt atcctggctg accagcatcc ggcgtagtgc cgcagcacgg
    17101 cgtggaacgc gcagtttgcc aggcaacacg ccttcggtgg aaataccgcg ctcaataccg
    17161 ccacgcatga cttcccagac gttcgccagg tgctgttcca gctcttcttt actgtgcagc
    17221 gccagctcgt ttttcatcat caggccagag agtgacagcc cggtttcctg acaatgtttt
    17281 tgcagatcgg ctgctgaact gtacggataa ggaacttcaa ccggtgcgct atcctgctgg
    17341 ccaaaatgct cttcatcaac gataaagcca ccgccaatag agtagtaagt ctggctgtaa
    17401 acgactttat cgcccgccag cgcggtaatg cgcataccgt tttcatgcag agaaaggttg
    17461 tctgcatgga agttcatgca ctgatcaacc gggaactcca cttcatgctg accgtttgcc
    17521 agcatcaggc gaccatgagt attcacatcc tgaataaaac cggggatgga atcgatatcc
    17581 acggtatccg gcaggttacc cgccaggccc ataataatgg agatatctgt gtggtggcct
    17641 ttaccagtca gagagagcga gccgtacacg tcaaccacca cgcgggtcac gtctttaagc
    17701 aggttacggg caatcagatc gtcggtaaat tgtttacccg ctttcattgg tccaacggta
    17761 tgagaactgg aagggccaat gccgattttg aaaatatcga atacgctaat cataggaaat
    17821 acatcgcgtt aaaacggagg aagcgccgcc cgaaagcggc gcgaaaggac ttagctgaac
    17881 agagagtaga agattgcgga gattgcaatc agccccatca cgacaacgaa tacgttgctg
    17941 atgtgaccgc tgtacttacg cattgccggt actttctgaa ttgcgtacat cggcatcagg
    18001 aacaggatca tcgcgatgat tgggccgccc agggtttcaa tcatacccag gatgctcggg
    18061 ttcagggtgg caacaatcca ggtcgttacc agcatgaaca gcgcagtgat acggttcagc
    18121 ttgttgattt cgatagactt acctttacca cgcagagatt taatcaccat accgttgaag
    18181 ccttcacgtg cgcccaggta gtggccgagg aaggatttgg tgatagcgat aatcgcgata
    18241 atcggagcca tccacgcgat aaccggtgcg ttaaagtggt tagccaggta agacagaatc
    18301 gagatgttct gctctttagc cgcagccagg tctgccggag tcaggctcag tacgcagctg
    18361 aagacgaaga acattacggt cagcaccatc atgatgtgtg cgaatgccag gatcttcgag
    18421 catttctgtt ctgccatatc gccgtactct tcacgcttcg caacggcgaa agaagagatg
    18481 atcggagagt ggttgaacga gaacaccatt accggaattg ccagccacag ggtcatccac
    18541 agaccgtttc cggttgcaga tgcagtgtcc agagacagcg tttccagtgc tgcgccgttc
    18601 cactgcggga tcaggtacag agccagcagc atcagtacgc caacaaacgg gaataccaga
    18661 atactcatcg ctttaacgat catctgctca ccaaagcgaa cgatggtcat catacccacg
    18721 atcaggatca gcgacagaat cgcacgcggc ggtggcgtca tactcagctg gtgagacatg
    18781 aagctttcaa cggtattggt gattgccacg ctataaacca gcaggatcgg gtagatagcg
    18841 aagaagtaga gcagggtaat cagtttacct gcgccaatac caaagtgttc ttctacaacc
    18901 tcggtgatgt cttcgcccgg gtttttacca gacagtacga agcgagtcag gccgcggtga
    18961 gcaaaaaacg tcatcgggaa cgcaaggata gccatgatga tcagcgggat cataccgcca
    19021 acaccggcgt tgattggcag gaacagcacg cccgcgccga ttgccgtgcc gtaaaggccc
    19081 agcatccaca tggtgtctgt cttgcgccag gcactacgag agtctttcga cgcaatcgtg
    19141 ctggtttgag tcgtttccat ctatttctcc tggaggaatg caaataattc agcttttagc
    19201 cagttcagcc cgggaaggtg cctgaccgta aaaatgaaat ctgtctatcg gcgatggctt
    19261 aatcatttac cgctgtaata atttgagggc ggaaagatac acacaagtta tgtatctatc
    19321 agtgatcttg atcccaaata caataatctg cttggaagat agattatccc aacgctttac
    19381 tctgtaaatc aaaagtaaaa gagaatttaa gggcaatatt tgcccattaa acgacatctg
    19441 acttcttccg gggcgcgcaa tatatgtagg ttgaactgaa aaaatcaata cttttagtta
    19501 atcatggcga taatttcttt ttcatagcga taaaatacgg tttatgaaaa tagggaaacg
    19561 tttgcgttgc aacataaatt gtatggcata ttaactgtga tgatatttgt cgcgtgaggg
    19621 tggtaagaaa cattcatata acaaatgaaa tgagttgatg agtaagtgac caaaaacgag
    19681 aaaacccggc aacaaacgtc accg
//
LOCUS       DADMEQ010000071        19376 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.071, whole genome shotgun sequence.
ACCESSION   DADMEQ010000071 DADMEQ010000000
VERSION     DADMEQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.071"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(30..1859)
                     /gene="glmS"
                     /locus_tag="JNZ05_002523"
     CDS             complement(30..1859)
                     /gene="glmS"
                     /locus_tag="JNZ05_002523"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAY8565716.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2021..3391)
                     /gene="glmU"
                     /locus_tag="JNZ05_002524"
     CDS             complement(2021..3391)
                     /gene="glmU"
                     /locus_tag="JNZ05_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAY8565717.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3743..4162)
                     /gene="atpC"
                     /locus_tag="JNZ05_002525"
     CDS             complement(3743..4162)
                     /gene="atpC"
                     /locus_tag="JNZ05_002525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAY8565718.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="JNZ05_002526"
     CDS             complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="JNZ05_002526"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAY8565719.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="JNZ05_002527"
     CDS             complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="JNZ05_002527"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAY8565720.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="JNZ05_002528"
     CDS             complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="JNZ05_002528"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAY8565721.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="JNZ05_002529"
     CDS             complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="JNZ05_002529"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAY8565722.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="JNZ05_002530"
     CDS             complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="JNZ05_002530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAY8565723.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKLRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="JNZ05_002531"
     CDS             complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="JNZ05_002531"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAY8565724.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="JNZ05_002532"
     CDS             complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="JNZ05_002532"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAY8565725.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="JNZ05_002533"
     CDS             complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="JNZ05_002533"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HAY8565726.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="JNZ05_002534"
     CDS             complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="JNZ05_002534"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAY8565727.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="JNZ05_002535"
     CDS             complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="JNZ05_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAY8565728.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="JNZ05_002536"
     CDS             complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="JNZ05_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HAY8565729.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="JNZ05_002537"
     CDS             complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="JNZ05_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HAY8565730.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15345..16337
                     /gene="asnA"
                     /locus_tag="JNZ05_002538"
     CDS             15345..16337
                     /gene="asnA"
                     /locus_tag="JNZ05_002538"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAY8565731.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPVAVRESVPSLL"
     gene            complement(16342..17793)
                     /gene="viaA"
                     /locus_tag="JNZ05_002539"
                     /pseudo
     CDS             complement(16342..17793)
                     /gene="viaA"
                     /locus_tag="JNZ05_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
     gene            complement(17787..19283)
                     /gene="ravA"
                     /locus_tag="JNZ05_002540"
     CDS             complement(17787..19283)
                     /gene="ravA"
                     /locus_tag="JNZ05_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HAY8565732.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLKASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4627 a         5120 c         4859 g         4770 t
ORIGIN      
        1 cggcagccct tttttatcta tgatagtgat tactcaaccg taaccgattt tgccaggtta
       61 cgcggctggt caacgtcggt gcctttgatc agcgcgacat ggtaagccag cagctgcagc
      121 ggaacggtgt agaagatcgg tgcaatcacc tcttccacat gcggcatctc gatgatgtgc
      181 atgttatcgc tacttacaaa acccgcatcc tgatcggcga agacatacaa ttgaccgcca
      241 cgcgcgcgaa cttcttcaat gttggatttc agtttttcca acagttcgtt gttcggtgca
      301 acgacgataa ccggcatatc cgcgtcaatc agcgccagcg ggccgtgttt cagctcgcct
      361 gcagcgtagg cttcagcgtg aatgtaagag atctctttca gcttcaatgc gccttccagc
      421 gcgattgggt actgatcgcc acggcccagg aacagcgcgt gatgtttgtc agagaaatct
      481 tctgccagag cttcaatgcg tttatcctga gacagcatct gctcaatacg gctcggcagc
      541 gcctgcagac catgcacaat gtcatgttca atggaggcat ccagaccttt caggcgagac
      601 agcttcgcca ccagcatcaa cagcacagtt aactgagtgg tgaatgcttt agtggacgcc
      661 acgccgattt ccgtacccgc gttggtcatc agcgccagat cggattcgcg caccagagaa
      721 gaacccggaa cgttacagat tgccagcgaa ccaaggtaac ccagctcttt cgacagacgc
      781 aggcccgcca gggtatccgc ggtttcgcca gactgtgaca aggtgatcat caggctgtta
      841 cgacgcacgg cagatttacg atagcggaat tcagaggcga tttcgacgtc gcacggaata
      901 cctgccagcg attcaaacca gtagcgggaa accataccgg agttataaga agtaccacag
      961 gcgaggatct gaatatgctc aaccttcgac agcagttcgt cggcgttcgg tcccagctcg
     1021 cttaaatcaa cctgaccgtg gctgatgcgc ccggtaaggg tgttttttat cgcgttcggc
     1081 tgttcgtaga tctctttctg catgtagtga cggtaaatac ctttatcgcc cgcgtcatat
     1141 tgcagattgg attcgatatc ctgacgtttt acttccgcgc cagttttatc gaagatgttt
     1201 accgaacggc gagtgatttc cgcaatatcg ccctcttcaa ggaagataaa gcgacgggtc
     1261 accggcaaca gcgccagctg gtcagaagcg ataaagtttt cgcccatacc caggccaatc
     1321 accagcggac taccagaacg tgccgccagc agggtatccg ggtgacggga gtccatgatc
     1381 actgtaccgt acgcaccgcg cagctgcggg atagcacgca gaacagcctc acgcagagtc
     1441 ccgccttgtt tcagctccca gttcaccaga tgggcaatca cttcggtgtc ggtttcagaa
     1501 acgaaggtat agccacgcgc ttttaactct tcacgcagcg gttcatggtt ttcgatgatg
     1561 ccattatgca ccaccacaat gtgttcagaa acatgcggat gcgcgttagc ttctgaaggt
     1621 tcaccgtggg tcgcccagcg agtgtgagca ataccggtgc cgccatgcag aggatgttct
     1681 tccgctgcct gagccagcat ctggacttta ccgaggcgac gcaggcgagt catatgacct
     1741 tccgcatcaa caacggccag accggcagag tcatatccgc ggtattccag acgacgtaaa
     1801 ccttcaagaa ggatttctgc tacatcacgt tgcgcgatcg cgccaacaat tccacacata
     1861 gtttttgatt ccgatttata tcgttgtcgg tcaacctgta tgcccgtttt ttcgggcgcc
     1921 ccgagccttg tagagagtgg ggttattttt atagttactg cttgtgggag ggagattatg
     1981 ttatctcctc atcccagatg accggattac ccggccagaa ttactttttc tttaccggac
     2041 gacgccagcc ttctttctga gtctgcggca cacggctgat agctaatgca ttttcgccga
     2101 cattacgcgt cacagttgta cccgcagcaa tggtcgcgcc tttgcctact gttaccgggg
     2161 ccaccagctg agtgtcggaa ccgacaaaca catcgtcgcc gataatggtc ttaaatttat
     2221 tcgcaccatc gtagttgcag gtaatggttc ccgcgccgat gttaacgtta tcgccaattt
     2281 ccgcatcgcc taggtaagtc agatgcccgg ctttcgtgcc tttacccaga cgcgcttttt
     2341 tcatctcaac gaagttacca acgtgtgcac cttccagcaa ctcagcacca ggacgcagac
     2401 gggcaaacgg gccaatagta caggccgctg ccagattcgc gtcttccaca acggtatacg
     2461 gactgatttc gcaatcatcg ccaatcacgc tgtttttaat cacgcaaccg gtgccgattt
     2521 tcacgcgatg accgagagtc acgttgccct cgatgataac gttagtatca atttcaacat
     2581 cgcgcccgtg agtaagcgta ccgcgcagat caaaacgcgc cggatcgcgc agcataacgc
     2641 ctgctaacag cagtttttca gcctgttcgg actggtaaac gcgctccaga cgggagagtt
     2701 gcaggcggtt attcacacct tctacttcgc ttaaacgttg cggatgaacg gcgacgattt
     2761 cacgcccttc ctgatacgcc agcgcaataa tgtcggtgat gtagtattcg ccctgagcat
     2821 tattgttggt cagcttcgcc agccagcgtt tcatatctgc gccgttggca atcagaatgc
     2881 cggtgttgat ctcctgaatc tgacgctgct cgtcggtggc atctttgtgc tcaacaatgc
     2941 cggtaacttt gccattttca cgggtgatac gtccataacc ggtcggatca tccagtttca
     3001 ccgtcagcag accaatgcca ccctggggtt tagcatcacg caggcgctgg agtgtttcga
     3061 cagagatcag cggcacgtcg ccgtagagca ttaaaatgtc ttcatcatcg gcaaagaaag
     3121 gtgcggcctg ctgcattgca tgacccgtac ccagctgctc tgcctgaagc acccagttaa
     3181 ggttgtcgtc tttcaacgcc tgtttaagca gatcgccgcc gtgaccgtac accaggtgaa
     3241 cgtgcgctgc gcctaattca ttcgcagcat caatgacatg ctgaaccatc gctttcccgg
     3301 caagggtatg cagcactttc ggaagatcgg aatacatgcg cgtgcctttg cctgcggcaa
     3361 ggatcactac gctcatagca ttattcaaca tacgcgtcct gactgtaatt tgagaacgaa
     3421 tttaaaccgc ttcaccttga aaaaactaca tttttttcat cgtgaaatgg acagaggata
     3481 aattgttcaa taacggatta tccccgctga cgaaagtgcc attttcgacc atcgtcacgc
     3541 cttttgtctg cagaaaatta aggcagtacc ttcatttaag cgtcaggtgg atgtttttgc
     3601 tcttatttcg atcaatgaat aaacgggaaa acaggggtgt tttatatcag caggatctat
     3661 gtgaacgctc ttcaggacgg gtcaacgcgt aaaaaaaagc cagcctgttt ccagactggc
     3721 ttttgtgctt ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct
     3781 gcgcgatcgc tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc
     3841 tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct
     3901 ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc aggctgcact tcaagaatgc
     3961 cgccagacag atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag
     4021 gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac
     4081 ccgttacctg gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt
     4141 ccaggtggta agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt
     4201 ttccacagct tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta
     4261 ttcgccttcc atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc
     4321 cggagaaccg gtgaatactt ctgccacgaa gaacggctgg gacaggaagc gctggatctt
     4381 acgagcacgc gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaggatggc
     4441 gatgatgtct ttcagttcct gataacgttg caggatggac tgaacgccac gcgcggtgtc
     4501 gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc
     4561 aacggccggg tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag
     4621 gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca tccgcaggta cgtataccgc
     4681 ctgtacggag gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacgcccat
     4741 ctcttccgcc agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga
     4801 tacttccgta ccggccaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg
     4861 accttcgtca cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt
     4921 tcccggcggc tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc
     4981 ggtcatttcg tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac
     5041 agagtaaccg gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt
     5101 acctacaccc gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag
     5161 gtcgataact ttgataccgg tttccagcag ttcctgagag tttgacagct cttcgtagga
     5221 aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac
     5281 cggttcaccc agtacgttca tgatacggcc cagagtcgct ttacctaccg ggacttcgat
     5341 cgggtgttcg aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc
     5401 gatggtacgc acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt
     5461 accattttgc acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac
     5521 gtcaactacg gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg
     5581 aaataacctg tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg
     5641 ctggcctgac gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg
     5701 tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc
     5761 acgccctgat aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc
     5821 ggatcgggtt cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc
     5881 ggtaacggca gcagctggct gatggtcgga acctgagaca tggtgttaat aaatttgttg
     5941 ctgacaatgt aaagcttgtc cagacggcct tcgtcgtagg cctgcaacat cacttttacc
     6001 ggaccgatca gttcggacag ggaagggtta tcccccatgc cggtgacttg ggcaacaaca
     6061 ttgccgccca cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat
     6121 tgaacgcctt tgtcggtcca ggtcttcatt tccgccagca gttttttgaa caggttaatg
     6181 ttcaaaccac cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg
     6241 tcgcggtctt ccaggtaagg gtgcttatat tccagattac cgtgtgcaag gtgaccaatc
     6301 actttgcgca tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc
     6361 attttggaag cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt
     6421 gcgatcttac tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc
     6481 ctgccctaag gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga
     6541 tgcctttcag cttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca
     6601 tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccaattt
     6661 tcgacagttc aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgct
     6721 gcgcaacgga catcggcgca tactgtttct gtttcagcag ttcggtcact ttctgaccgt
     6781 ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc aaactgagag aacgctgcca
     6841 gttcacgata ctgtgccaga gcggtacgga taccaccgga cagttttttc atgatcttgg
     6901 tctgtgctgc accaccaaca cgggatacgg aaatacccgg gttaaccgca ggacgaatac
     6961 cggcgttgaa caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg
     7021 tcggaacgaa cgcagaaacg tcacccgcct gagtttcgat aatcggcagt gcggtcagag
     7081 aaccggtttt ccctttcact tcacctttgg tgaaggcttc aacgtattcg gcgttaacac
     7141 gtgcagcacg ctccagcaga cgagagtgga ggtagaaaac gtcgcccggg aatgcttcac
     7201 gtcctggcgg acgacggagc agcagggaga tctgacggta agcaacagcc tgtttagaca
     7261 ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg gaagtattcg cccattgcgc
     7321 aaccggcata cggtgccagg tattgcagtg cagcggattc agacgcggtt gctaccacaa
     7381 cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg
     7441 acgctttctg gccgatagcg acatagatac atttgatacc ggaatcgcgc tggttgatga
     7501 tggcatcgat agccagggcg gttttacctg tctgacggtc accgatgatc aattcacgct
     7561 gaccacgacc gattgggatc atggagtcaa cggctttata accggtctgt accggctgat
     7621 ctacggactg acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt
     7681 gatccagcgg acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca
     7741 ggccacggcc aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg
     7801 caaggtcagc gtacggaccc ataacaaccg cacctacaga gtcgcgctcg aggttcagtg
     7861 cgatagcgta acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggccaggc
     7921 cgtgaatgcg gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac
     7981 tcacaacatt gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca
     8041 gttgcatgct ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc
     8101 gtacgctgcc atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact
     8161 tatcgatttt gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa
     8221 ttttcgcgag ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag
     8281 cctcactcac ggcacgaagg tgaataaact gctccagaac atccgggagc gcgttaagac
     8341 gaccattttc agccattacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac
     8401 aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag agaagctctg
     8461 ccatttgttc gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta
     8521 cactttggtg ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa
     8581 taaattcaga catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc
     8641 gctgttagca gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag
     8701 gatagcaact tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttctgc
     8761 ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag
     8821 aatctgcgag cggagtttat tcgcctgctc gatgattacc tgggcttccg ctttcgcttt
     8881 tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc
     8941 ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg
     9001 ccatacgtac ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat
     9061 tgttgcgtta agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt
     9121 taaataaaag caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag
     9181 cgatcatcgg gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag
     9241 gaatcagatc aggttgacgc gctgcgcctt ccaggaattt acccccgagg atgccgatac
     9301 cgatcgcagc accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat
     9361 ccatattcag gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg
     9421 gtaaattaat gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg
     9481 aaggcttgca gcgtaatgat caggatgtgg aaaatggccc acggcacatt caggatccac
     9541 tgtgaccacc acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg
     9601 ttaccgaaca gtcgcaaacc gagtgaaact ggtttggaca gcaggcttac cccttcaagg
     9661 attaagttga caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg
     9721 aagccgccga tgcctttcat tttgatgttg tagaacagaa tcaggataaa tacgcccagt
     9781 gccatagaca gcgttacgtt cacgtccgca gacggaacca cacgcagtgc aggcagaccc
     9841 agtacatgtt cagcaatgta cggcagcaag tcgataggca gtaaatccat cagattcatc
     9901 aggaataccc agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg
     9961 tacatgtctt tcacgctacc attaacaaag ccgatcacca gctcaatcgc ggtctgaaac
    10021 ttacctggca caccgctggt cgcctttttg gctacgctac ggaataaaac caagaacaac
    10081 agacccagca ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg
    10141 gggttttgtg gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg
    10201 tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt
    10261 tgttaattac agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca
    10321 gcggcaagaa taccgccttt aaaaccgcca acgccaccac cagtaacacc aacatcgcca
    10381 gaactttgaa agcttcgcca aatgcgaatg tccaagccac ccggcctttc gctggtgtat
    10441 gcgcctggtg acgccaggca aatatcataa acaaaacgtt aggcagaaag actgccaggc
    10501 ccccgcttat tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg
    10561 ctatcaccac cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg
    10621 acacagacat cacgtttttc actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa
    10681 ctttctttaa ggcttagagt caagcatcaa aaagcggtca aattatacgg tgcgcccccg
    10741 tgatttcaaa caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat
    10801 acaattgcga cttttccgct acccctgttc gatcatgaaa aactgtaaat aacgcgtaaa
    10861 cactggtgat aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac agcatattta
    10921 tttacttggc aaatgatgcc tttgcaagtt tatgatattt cagtctaaaa acagatactg
    10981 ttttaataaa tgacatttac acaacaaaaa ccacccattg acatttttaa taatgtttta
    11041 acagccaatg atggttctta gcgccgattt ttagcagact gatattttca ctaatgactt
    11101 attttctgct taccaaaaaa agccacgtta tcttgttgat gcaaaagagt gaacgtggcg
    11161 ttaaatgtaa ccagttatat cagtagaaaa cctggttgtt gttaacagtc taaccggtca
    11221 attttttatg atttttttga taaaaattaa attttatttg ctttaatcac caccagatga
    11281 cgttcgccat ccagggctgg aacctgaagt ttaaccactg attcgacctg atattcttcg
    11341 ggcaacaaag cgatttcatc ttccggcatt tgccctttca gcgcgtagaa acggccttgc
    11401 tcaccaggaa gatggtggca ccagctcacc atatcgttca gagaggcaaa agcgcggcta
    11461 attacgccat caaatggcgg ctctgaagga aactcttcta ccctgctctg tactggttca
    11521 atattctcca gtttaagctc atgttgcacc tgacgaagga aacgcacgcg tttaccaagg
    11581 ctatccaaca gagtgaaatg ggcttcagga cgcacgatag agagtggaat gcctggcagt
    11641 cctggtccgg tgccgacatc gataaaccgt tcaccttgca gatacggtgc caccacaatg
    11701 ctatcgagaa tatggcgtac cagcatctca ttaggatcgc ggaccgaagt caggttgtac
    11761 gctttgttcc atttatgcag catattcacg taggcaataa gctggttttt ctggtgatca
    11821 gtaagcgaaa tacctgcgtc tttcagcagt aaggagagtt tgttgagcac ggtgattacc
    11881 tgttcttgat gcgttgcctg gtaagcgggt gcttaccagg catttttaat gcgttatgcg
    11941 ctacgacgca gcataccctg ttttttcagc cacaccagca gaatggagat ggccgcaggc
    12001 gtgacgccag aaatacgcga agcctggccg atagaggccg gtttgtgatc gttaagtttg
    12061 gcgatcactt cgttagaaag accggatacc tggcggtaat ccagtgtcgc gggtagcagg
    12121 gtgttctcgt tacgcagctg cttttcgatc tcatcttgct ggcgcgcgat ataaccttcg
    12181 tatttaacct gaatctcaac ctgttccgcc gcctgttcgt ctgtcaacgc aggggcaaac
    12241 ggcgtcagcg tggttaattt ttcataagtc atttccggac gacgcagcag atcttcacca
    12301 ctggcttcac gggaaagcgg cgcagtcagg tgagcattca cttcggctgc agcttccgcc
    12361 gacggggtta cccaggtcga tttcagacgc tgacgctcac gctcgatatt ctcaagtttc
    12421 tcgttaaagc gcgcccaacg ttcgtcatcc accaggccca gttcacgacc gatttcagtc
    12481 aaacgcagat ccgcattatc ttcgcgcagc atcagacgat attctgcgcg cgaagtaaac
    12541 atacgatacg gttctttggt tcctaaagtg cacaggtcat caaccagtac gccgagatac
    12601 gcctgagaac gtgccggagc ccaaccttct ttgtcagcag acagacgggc agcgttaaga
    12661 ccggccagca agccttgcgc agcggcttct tcgtaaccgg tagtgccgtt aatctgacca
    12721 gcaaagaaca gcccctggat aaacttgctc tccagcgtcg gtttcaggtc gcgaggatcg
    12781 aagaagtcat actcaatggc ataacccgga cgcacgatct tcgcgttttc catcccctgc
    12841 atagagcgga cgatttgcat ctgcacatcg aacggcaggc tggtggagat accgttcgga
    12901 taaatttcat tagaggtcag tccttccggt tcaaggaaga tctgatgctg atttctgtct
    12961 gcgaagcgca tgactttgtc ttcgatcgac gggcagtagc gtgggccgac accttcgatc
    13021 acccctgcat acattgggct acgatcgagg ttactgcgga tcacatcatg ggttttctcg
    13081 ttggtatgag tgatataaca cggcacctgc tggggatgca gggacgcatt gcccataaac
    13141 gagaataccg gcattgggtt atcgccatgc tgttgcgcca gcacgctaaa gtcgatggtt
    13201 cgagcatcaa tacgcggcgg agtaccggtt ttcagacgac caacgcgcag cggcagttca
    13261 cgcaaacggc gagagagcgg aatggatggc ggatcgccag cacggccacc gctgtaatta
    13321 tccagaccga tatgaatttt gccgtcgagg aacgtcccaa cggtgagcac cacagctttg
    13381 gcacggaact tcagtcccat ttgggtaaca gcaccgacca cgcgatcgtt ttcgacaata
    13441 agatcttcaa ccgcctgctg gaagatcatc aggttcggtt ggttctccag cgccgtacgt
    13501 accgcctgac ggtagcgcac acgatccgcc tgagctcggg tagcgcgaac cgccggtcct
    13561 ttgcttgcgt ttagtatcct aaactggata cccgcttgat cgatcgcttt cgccatcaga
    13621 ccgccgagtg catccacttc ttttaccaga tgtcccttcc caataccgcc gatcgccggg
    13681 ttgcagctca tctgccccag agtgtcgata ttgtgtgtca aaagcagagt ctgttgaccc
    13741 atacgcgccg cagccatcgc ggcctcggtg cctgcatgac ccccgccaat gatgatgacg
    13801 tcaaaaggat ccggataaaa catggtgatt gcctcgcata acgcggtatg aaaatggatt
    13861 gaagcccggg ccgtggattc tactcaactt tgtcggcttg agaaagacct gggatcctgg
    13921 gtattaaaaa gaagatctat ttatttagag atctgttcta ttgtgatctc ttattaggat
    13981 cgcactgccc tgtggataac aaggatccga cttttaagat caacaacctg gaaaggatca
    14041 ttaactgtga atgatcggtg atcctggacc gtataagctg ggatcagaat gaggggttat
    14101 acacaactca aaaactgaac aacggttgtt ctttggataa ctaccggttg atccaagctt
    14161 cctgacagag ttatccacag tagatcgcac gatctgtata cttatttgag taaattaatc
    14221 cacgatccca gccattcttc tgccggatct tccggaatgt cgtgatcaag aatgttgatc
    14281 ttcagtgttt cgcctgtctg ttttgcaccg gaatttttga gttctgcctc gagtttatcg
    14341 atagccccac aaaaggtgtc atattcacga ctgccaatac cgattgcgcc aaagcggacc
    14401 gcagaaagat cgggcttctg ttcctgcaat gcttcataga aaggagaaag gttgtccgga
    14461 atatctccgg caccgtgggt ggagctgata accagccaga tccccgaggc agataaatct
    14521 tccaacagcg gaccgtgcag cgtttcggtg gtaaaacccg cctcttccag cttttcagcc
    14581 aggtgttctg ctacatattc ggcaccgccg agggtgctgc cgctgataag agtgatatct
    14641 gccataaacc gccaccttta ttaagagtgg cgtattgtac gctgtgaact cgttgggatc
    14701 tacctgtgga aaagtatggg attaaaaaag ccgatcaggg cttgatggta cgcatgattg
    14761 ggttttgcag gacgatcaat gtctcggtgg actgaatttc atcaattgtt tggatcttgt
    14821 tgataagtac atgctggaga gcgtcgatcg aacggcacat aacttttata aagatgctgt
    14881 agtggccggt tgtgtaatag gcttcagtga cttcatcaag gctttccagc tttgccagcg
    14941 cggaagggta gtctttggcg ctctttaata taatgccgat aaagcagcct acgtcataac
    15001 cgagctgctt cgggctgaca tcaatacgcg ccccggtaat gatccccgcc tgcttcattt
    15061 tctctactcg aacgtgaatc gtccccggac tgacgccaaa ttgtttcgcc agttcggcgt
    15121 aagcggtgcg cgcattgccc attaatgctt ccaggatgcc acggtccaga ttgtcgatca
    15181 gataattttc cataggattt tcttatgcag attgatgatt cattctattt tagccttctt
    15241 ttttaatgaa tcaaaagtgt gttaggcttt ttattgaatg attattgcat gtgtgtcggt
    15301 ttttgttgct taatcataag caacaggacg caggagtata aaaaatgaaa accgcttaca
    15361 ttgccaaaca acgccaaatt agcttcgtga aatctcactt ttctcgccaa ctggaagaac
    15421 gtctggggct gatcgaagtc caggcgccga ttcttagccg tgtgggggat ggcacgcagg
    15481 ataacttgtc gggctgtgaa aaagcggtgc aggtaaaagt gaaagctctg cctgatgccc
    15541 agttcgaagt ggttcattca ctggcgaagt ggaaacgtca gaccttaggg caacacgact
    15601 tcagcgcggg cgaagggctg tacacgcaca tgaaagccct tcgccccgat gaagaccgtc
    15661 tttctccgtt gcactcggtc tatgttgacc agtgggactg ggaacgcgta atgggcgacg
    15721 gtgagcgtca attctcgact ctgaaaagca cggtagaggc gatctgggcg ggaattaaag
    15781 caaccgaagc tgcggttaac gaagagtttg gcctggcacc gttcctgccg gatcagattc
    15841 acttcgtaca cagccaggag ttactgtctc gttatccgga tctcgatgcc aaagggcgtg
    15901 agcgggcgat tgcgaaagat cttggcgcgg tattcctcgt cgggattggc ggcaagttga
    15961 gtgatggtca tcgtcacgac gtgcgcgcac cggattatga tgactggagc accccgtcag
    16021 agctgggcca tgcgggcctg aacggcgata ttctggtgtg gaacccggta ctggaagatg
    16081 cgtttgagct ttcttccatg gggatccgcg tggatgccga cacgctgaag catcagctgg
    16141 cgctgaccgg tgatgaagat cgtctgcagc tggaatggca tcaggcgctg ctgcgcggtg
    16201 aaatgccgca gaccatcggc ggcggtatcg gccagtctcg tttgactatg ctgctgctgc
    16261 aactgccgca tatcggccag gttcagtgtg gagtatggcc agttgctgtt cgcgagagcg
    16321 tcccttctct gctgtaataa tttatcgccg ccagcgtctg agcaggcggc ttcgcatccc
    16381 ggtatcaaag cgccagatat gatcgaaaat gcgcatgatg ccgggtttgc cgtgtgccga
    16441 catcgccacg gcatgaaagc gatgctgatg tacccgctgc agctctttca ctttactcgt
    16501 cacgtcgtca ggcaaccgct gagcgataaa atcagaaatc accaccgcat cggcatcaaa
    16561 ccattccctg ctttgcaagc gttccataat ggcgcgaaaa caactggcaa gatcggtgcc
    16621 gctacgaaac tgctggctta aaaaacggat tgcttgttcg atgccttgtg ggcctgaaag
    16681 ctcataacgg acgatctcgg tggaaaatag cataatataa cagcgccggt tttcagcgag
    16741 agcaatgcgc atcaaagcca ggcagaacgc tttcgcacac tgttcattaa agccgcccat
    16801 tgagccggaa gtatccacac agacaataaa cggcccgcgc ggctgttcat cgtaatcttt
    16861 atgtaccacc ggacgttcga gcactttttc acgccacgac tcaccgtgca ggcgataggt
    16921 gagcaactgt ttttccacca gccgacggta aaactcatac tccagttccg ttatccctag
    16981 tgtcgccagt tctggcggca ggagacgtaa aatatcatcg ctttgttgca gaccatcaac
    17041 ctgctcagga accgtcgccg gttcgcgcac catggtgcgg aaggtttcca tctacgcatc
    17101 gttgcgcggt attgatttgg cttcccgaga acgccccagc tgctctgcca ggcgtttcag
    17161 ttccggctgt tcgttaagaa attcaccgta tttcacaata aactgatagt cgccacgttt
    17221 aagctgaccg gcgctcatat cccacagacg accagctgcg gtattgttat ctgcgagaat
    17281 cggttcaagt tgtccgctca gcgtcatgcg ttcctgaact tcacttaaca gttgttcgcg
    17341 ttcttcttct aatagctgtt gatttaacgt cgttgcttgc acgatcagac ttaaacgcca
    17401 acgctggaga aaaagcgtgt gtaacgctga agttattgtg ctgttagcat caaccaactg
    17461 ccgggcttgt tctgcccatg gggaattcag acgatgcagt aagtctagga tctgtggtag
    17521 ctgcacaata aactgcggcg tggagaggag ctggctttgc tgatagcaca tcacctcttc
    17581 ggtgagttcc ggcgggactc gggcatcttt cagccgactg cgcagcgcct cacgccagcg
    17641 gggaacatca tcagtgattg ccgccttcag tcgtgggaat ttttcaaaga agaccgccag
    17701 ctgcggtgag gccagcagtg cgatgatcat ctcttcgatc aatccctctt cgctgacggc
    17761 cagcatcaca ttaagcgtat ccagcgttag cattgttgtg cctggcgaat ctgtgcaccg
    17821 acatcctgta ggctggcttc aatgcgacct aaccagtcgc cagggataaa cagacatttt
    17881 tgctgttcgc tgaataaagc gtgttgctta cgccagtcgc tttcaagctc ttccagttgt
    17941 tgttttattt cacctggcag accttcagcc gatgaaccgg gaagtgccag cgtactgcct
    18001 tgtaaactta catcgcgtac aacaagatgt tgggtgctat caacttccag attcagtttc
    18061 tgggcaaagc cgataccgtt cagtttgccg cgaatttcac cacctttact cagccactgt
    18121 tccagcgcgc tacgctcaaa ggagatatga accacttcca tatcatgcag ttttaacggt
    18181 ttttgcagca gcagagtcag agtggaagca gtaacgttaa caggaagttg atactgctga
    18241 cgacggctga aaatgccgcc cagacgaatt aacgttaagg ctgttttatc gctttgttgc
    18301 tgctggagtt gcaggtgacg ttgcacaatc gcgcccaggc gggtcaacat cccttgctgt
    18361 tgccaggcat gaccggtcat caatacatca atttgttgtt gtatcaaatt caggctttgc
    18421 gcgtcatacc acaggcaatc tttcagcaaa atgagatcaa ccggggcaac agcactgcga
    18481 ccgctaaaaa aggcgctggc cttcaataag cggatcgctt ttttccagcg acgatccgag
    18541 acataaggcg catccggtaa tttatccagt tgctggcgca gcataaaaat cagctcaaat
    18601 acatgatcgg gcagcgtaat ttcaccaatc tctttctgcc agcgttcata ttcttcatct
    18661 gtgacctgca aggcatcagg aaccggattg tcgttttcat cctgttgact ggtcagcatg
    18721 gagcggaaat tcgctttatc ttgcacttta tctaaccata gacgaatcag catgcggtca
    18781 tataacgctt ccagactgct gtctgcttcc ggcagctcgt tggaggccgc caccagtaga
    18841 cgcatcggga ttttttctac gtgtgcaccg ttgcggaact ggcgctcgtt aatggcggtg
    18901 agcaaggtat taagaattgc cgggcccgct ttccagatct catccagaaa gacgatttct
    18961 gcttccggca ggtaaccgct ggttaaacgt tcatagcgcc cttcatcttt tagcgcctga
    19021 atagaaaggg gaccaaaaac ttcttccggc gtggagaagc gggtcatcag atattcaaac
    19081 gcgcgggcat tctgaaaggc gaattttaag cgccgggcga tcaaactttt ggcaatacct
    19141 ggcgggccaa ggaggaacac actttcacca cttaatgccg ctaataaaca caagcggatg
    19201 gcgtggctac gttcataaag ccccttttcc aacgaactgc tcaggcggga aattctttcc
    19261 gctaataaat gagggtgagc cataatgaag tggcgtcctt tcgtcaaaag ttctgcgtaa
    19321 attgcgagta tagacgtttc ttgctggtgg ctaaaatagt ctcaaagggg ggtaat
//
LOCUS       DADMEQ010000072        18259 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.072, whole genome shotgun sequence.
ACCESSION   DADMEQ010000072 DADMEQ010000000
VERSION     DADMEQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18259
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.072"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(62..820)
                     /gene="aroD"
                     /locus_tag="JNZ05_002541"
     CDS             complement(62..820)
                     /gene="aroD"
                     /locus_tag="JNZ05_002541"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAY8565733.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="JNZ05_002542"
     CDS             complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="JNZ05_002542"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HAY8565734.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1729..2943)
                     /locus_tag="JNZ05_002543"
     CDS             complement(1729..2943)
                     /locus_tag="JNZ05_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8565735.1"
                     /translation="MYFSYFLHGISVITLAQNMTSLAEKFSTDNAGIAYLISGIGLGR
                     LISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLTLAYGLAVCVGIANSALD
                     TGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYMLLNNIWYGYGLIIPGILF
                     VLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSSVLFGVAAFSTFYVIVVW
                     MPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKMVRPIWANVFNSALATIT
                     AAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFPKSKAKVTSIYMMMGGLA
                     NFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRYYRVFIIPENDVRFGERK
                     FSTRLNTIKHRG"
     gene            complement(3183..4328)
                     /locus_tag="JNZ05_002544"
     CDS             complement(3183..4328)
                     /locus_tag="JNZ05_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8565736.1"
                     /translation="MGVILMSLNMASLETLCQTNAAGVSIVISSLGIGRLSVLLFAGL
                     LSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFGFLAGMANSFLDAGTYPSLME
                     AFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWFGWSFMIAAGIMFINALFLYR
                     CTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGYISMATFYLVSQWLAQYGQFV
                     AGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTTLLMLYTFISFIALFTVCLHP
                     TFYVVIIFAFVIGFTSAGGVVQIGLTLIAERFPYAKGKATGIYYSAGSIATFTIPLIT
                     AHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHSLKENVAPGG"
     gene            complement(4487..4843)
                     /locus_tag="JNZ05_002545"
                     /pseudo
     CDS             complement(4487..4843)
                     /locus_tag="JNZ05_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            complement(4979..5086)
                     /gene="rprA"
                     /locus_tag="JNZ05_002546"
     ncRNA           complement(4979..5086)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="JNZ05_002546"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="JNZ05_002547"
     CDS             complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="JNZ05_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HAY8565737.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6773..9829
                     /locus_tag="JNZ05_002548"
     CDS             6773..9829
                     /locus_tag="JNZ05_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY8565738.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9826..10236
                     /gene="menI"
                     /locus_tag="JNZ05_002549"
     CDS             9826..10236
                     /gene="menI"
                     /locus_tag="JNZ05_002549"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HAY8565739.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10336..10524
                     /locus_tag="JNZ05_002550"
     CDS             10336..10524
                     /locus_tag="JNZ05_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565740.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11072..11440
                     /gene="sufA"
                     /locus_tag="JNZ05_002551"
     CDS             11072..11440
                     /gene="sufA"
                     /locus_tag="JNZ05_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HAY8565741.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEIDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11449..12936
                     /gene="sufB"
                     /locus_tag="JNZ05_002552"
     CDS             11449..12936
                     /gene="sufB"
                     /locus_tag="JNZ05_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAY8565742.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12946..13692
                     /gene="sufC"
                     /locus_tag="JNZ05_002553"
     CDS             12946..13692
                     /gene="sufC"
                     /locus_tag="JNZ05_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAY8565743.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETHDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13667..14938
                     /gene="sufD"
                     /locus_tag="JNZ05_002554"
     CDS             13667..14938
                     /gene="sufD"
                     /locus_tag="JNZ05_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAY8565744.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGFKQQVLARIGQRLPGGAR"
     gene            14935..16155
                     /gene="sufS"
                     /locus_tag="JNZ05_002555"
     CDS             14935..16155
                     /gene="sufS"
                     /locus_tag="JNZ05_002555"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HAY8565745.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALGCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16168..16584
                     /gene="sufE"
                     /locus_tag="JNZ05_002556"
     CDS             16168..16584
                     /gene="sufE"
                     /locus_tag="JNZ05_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HAY8565746.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16733..17737
                     /gene="ldtE"
                     /locus_tag="JNZ05_002557"
     CDS             16733..17737
                     /gene="ldtE"
                     /locus_tag="JNZ05_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HAY8565747.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17800..18036)
                     /locus_tag="JNZ05_002558"
     CDS             complement(17800..18036)
                     /locus_tag="JNZ05_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major outer membrane lipoprotein"
                     /protein_id="HAY8565748.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4801 a         4404 c         4691 g         4363 t
ORIGIN      
        1 acggttaaaa atactttttt tatttcggcg ggggagggta tttccgccga aatattattg
       61 cttatgcctg atgtaaaata gttaataccg tgcgcaaatc atttaccgag atttgccctg
      121 gcgcagaggc tttttttacc gcaccaaaag ttgccgccga gccaaatact tcaccagcca
      181 gacgagaaat tacgccagtt tttgccatcg acatcgtgat gattggacga tcggcatact
      241 gctcttgcat ctccagggtc gcggcaagca acgtcagcac atcgctggta ctttgcggca
      301 tcagcgcaat cttaggaata tcggcgtcga aggactgcat tttgcgcaga cgggcaatga
      361 tttcttcggc ttccggcgtt ttatggaagt catggttgga catgacaact ttcacatcat
      421 gcgcgtgggc gtaggcgacg gtttctttga cctgatcatc gccggtaaat aactccagat
      481 cgatcatatc aaccaggccg ctgtcgatgg ctgcacgatt gagagcaata taagcctcgg
      541 tggaaatcgc ctgctcgccg ccttctttgg cactgcggaa ggtaaacagc agcggttttt
      601 ctggcatggt ttcacggaga atttttgccg ccgccatgac agactccaca ttggagaggt
      661 cggcaaagtg gtccacacgc cattccagaa tatcaaagtc cgcttcacga taggcgagag
      721 cttcggattt cacgcgggcg atatctttcg ccatcagcga gacgatgatt ttaggtgcgc
      781 ccgcaccaat gacgagatct tttacagtta cggttttcat tttttaccct ttctgcacgc
      841 ggtcagcctg tcaggcaccg aaccccatga cctgtttaac atattccaga gggaaatctt
      901 tgccagtcca taacgtgaac tgttcagccc cttgccacaa caacatgccg tatccatcaa
      961 tcgttttgca accagcttgt tgtgcctgct gcaataactt cgtcatatgc gggttataca
     1021 cgcattcagt gaccagcagt cccggatgta acagactgat atcattaacc aatgattcat
     1081 tctcaagggg tttcatacct acttttgtgc cattggttaa aatgtcggcg gaagccaggg
     1141 cttcagcaaa ggcttgctga tcggcgagat cggtaaccgt gacgacacaa tcggtgtttt
     1201 cgttaacccg ctgcgcgaag gcgagggctt tatcgaagaa ctcatcccga cggttaaaga
     1261 gtttaatttc ttttaaacct tcaattgccc cctgcgcgcc aatcgccgtt gaggcacctc
     1321 cggcccctaa cagcaccatc gttttgcctt tgatatcaaa accgctctct ttaatggcgc
     1381 gaatatggcc cgtgccgtcg gtgttatagc cacgcagata gccatcatca ttaacgatgg
     1441 tgttgatggc acccaccagt ttggctgctg gcgttaattc atcaacatat tcacacgcca
     1501 gttgtttgtt cggcatcgac acaccagttc cgcgcatttt gagggctttt aatccttcaa
     1561 ttgctccggg aaagctatcg ttatccactt cgaaggccat ataggtaaat ggcaatcccg
     1621 ctttttctaa ggctttattc tgcatttcag gcgataaact gtggcggata ggataagcca
     1681 tcaatccaat caattcgtat tttgcggtaa catccataat taactccttt aacctctatg
     1741 cttaattgtg tttaaccggg tgctaaattt gcgttcgcca aaccgcacat cattctcagg
     1801 aataataaaa acgcggtaat agcggctaaa aacaataatt gcggtaatca gggccagcag
     1861 cgcgaaagta aaatcgagaa caatgatata ttgcaggccg atgttcgaca gataaccggt
     1921 aatcagcgga ataacaaagt tagccagtcc acccatcatc atataaatac tggtgacttt
     1981 ggctttgctt ttggggaaaa actctgacat gaccgaaaca ccgagctgta aaatgccgcc
     2041 agctgctgag aaaccgataa caaaggcgcc ggcattacac accagtggag aagggtacag
     2101 gtagataatg gcagctgtta ttgttgccag cgcagagtta aatacattag cccagatggg
     2161 ccggaccatt tttttcagta gtgcggcaaa aataaagaca cagaccaacg agcccatact
     2221 gtaataagag atggttttta atgcctcggc ttctgacata ccagcaaaag ccatcgcata
     2281 tttgggcatc cacaccacaa tcacataaaa ggtcgagaat gcggctacac cgaacagtac
     2341 cgatgaaaca ccttccagcc agactaacgg tttgctgttc atttgcggta attcattagc
     2401 tacgctggcg tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag
     2461 tacaaacaga atacccggaa taatcagccc atagccgtac cagatattat tgagcaacat
     2521 atagctcacc agcattgggt agaacatttg cccaaatgac accatcgctt taaccagtat
     2581 gaccgccgaa ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc
     2641 cagcgctgag ttagcgatac ctacgcacac tgccagaccg taggcgagag ttaaattcgg
     2701 gcaagcggga ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc
     2761 ccgacgacca aacttatcgg agatcacacc gaagaataaa atactgatca atcgccccaa
     2821 accgataccg gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact tttctgccag
     2881 agatgtcata ttttgggcaa gcgtaataac actaatgccg tgcaggaaat agctgaagta
     2941 aatacaaaga acagccagga taaatggcgt gctgaaagat aatttcgtta ccgtacgtat
     3001 tgttctccga cggtttacat aattactata ctgaagatga ttttccctgg gtacatggca
     3061 taaagtttat gtttccagga atattgcatg tcatagtttg tttttgatcg cgattttcct
     3121 tagttagcgc agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg
     3181 cattacccac ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg
     3241 cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag tatcgaacca cataatatcg
     3301 gcaatacttc tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg
     3361 cccgcactgt aatagatccc tgtagcttta cctttagcgt aagggaaacg ttcagctatt
     3421 aacgtcaggc caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat
     3481 attatcacca cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagata
     3541 aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta
     3601 ataaatacac aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg
     3661 tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac
     3721 gttgccattg agatatagcc atataaggta taactggcta aatcgataat tgaacagcga
     3781 tgttccgtag agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg
     3841 aacgtacaac ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag
     3901 gaccaaccga accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat
     3961 tgtccgctgg aaacaaatgc tttaattaaa atattggctg ttccaggtga gcgtggaaaa
     4021 gcttccatca aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga
     4081 aagccaaaaa cataagcgat aatgatgtta ttggtctgca ggatgccaaa aaagaaggcc
     4141 atatagcagc acattccgag catgataaaa gggcggcgac caaagcgatc ggataataat
     4201 cctgcaaaaa gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca
     4261 cccgcggcat tagtctgaca aagtgtctcc agcgaggcca tattcaggct catcaaaatg
     4321 acgcccatac catgcaccag gtaataaaat acaacccaag tgcggtaggg taatagggat
     4381 ttttcatagg ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat
     4441 ccattcatta ttgttgtaac aacgacaatt cctttctgtg gaaaaattaa caaagccgct
     4501 ccagatcgtg tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat
     4561 tgccatcagg gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt
     4621 tgccgatgcg gttatttatt ttctctatga tggcatgaag atgttttttt cgttactgac
     4681 gcatagccaa cagttgctcg actacacaat caggaatggc gcatttgcca ttttcccact
     4741 gacgccagct ttcactgtca ccggtctggg caatccaggt cgcgcattca tcaatagtca
     4801 tggcaaaaat atggcggagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg
     4861 aaataaacaa taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca
     4921 gtatcttgtg caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt
     4981 aaaaaaaagc ccatcgtggg agatgggcaa agactacaca cagcaattcg ttgtttcact
     5041 caggggattt ccatgcttat aaatcaatat gttgatttat aaccgtgagc taatagtagg
     5101 catacggact ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt
     5161 gcgacaggta ctaccagctg aatggcggta agcaggaaaa tatggggaag agcaagcaaa
     5221 tatcaattag cggtgattaa caatcaggcg gcagagccgc ctgatgagaa attacttatt
     5281 cggtttctcg atttcgccaa gttcttcgag aatttcttcc ggttggtccg tcggcggcgg
     5341 gacttcttcc acccacgcgg caaacagacg ccaggaaacg gctaacagaa ccggaccaat
     5401 aaacagaccg atcatcccga aagcaatcaa accaccaata acgccagaga ggatcaggat
     5461 cagcggtaaa tcggcaccca tgcgaattaa cattgggcgg atgacgttat ccagcgtgcc
     5521 aaccacaccg ctccacacta acaataccgt tccccaggtg gtatcgccgg tccagtagag
     5581 ccagataatc gccggaatca gtaccggcaa cgggccaagc tggacaaggc aggagaggat
     5641 cattaacacc gttagcagcg ttgcataagg tacgccggat acggcgaggc cgataccgcc
     5701 aagcactgcc tgtactaacg ccgtcaccac cacacccagc gccaccgcgc ggatagcctg
     5761 tgccgccagc agcacggcgg catctccgcg aacgcctgcc agacgggttg caaaatggcg
     5821 aatgccttgt gccacctgtt caccgcgcca gtacagcagg gcactgaaga gaagcatcag
     5881 cgcacaatgc accataaagc gcccgatatg cgccgcctgc ccaacgaacc aggtggtggt
     5941 ggtgccaata taagggcgga ctttcgccat gatcgccgta ccccccatat ccagcaagtt
     6001 gtgccagcct gcatacagct tcgcgccaat caccggaatg gtattaagcc acgccagatc
     6061 gggtaacgtc atgtcgccgc tggaaatggc tttaattagc gggccgctgc cgtcgacgat
     6121 actgttaacc agcaaagcaa tagggatgat aaacaccatc actaataaca gcgtcatcac
     6181 cagaacggcg agagagcggc ggccaaacat gatcttttgc aaacgtaaca ataccggcca
     6241 ggtggcgata accaccgtac cggcccatgc aaagccgaga ataaagggtt gaacaatcca
     6301 cagacatgcc acaatcatga tggctaaaaa cagcaccgaa agcagaattt gtgcgacatc
     6361 cctgggctga cgaacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg
     6421 acgcgacgtg gacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa
     6481 caggccgatt ctgtccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact
     6541 caggcaacac gcaaacgatt tactcgtcgt atttcaagtt gcatgtgctg cggctacgtt
     6601 cgttcacccc agtcacttac tattgtaagc tcctggagtt cactcgcttg ccgccttcct
     6661 gcaactcgaa atccatagag taaataaaca tcacctttca gtgcgctatt gcagccgcct
     6721 gaaagaatag tgtatcgacc tacatcacag accgcaggaa agggtcaata taatgattcc
     6781 acagatttcc caggcacccg gcgtcgttca actggtgctt aattttttgc aagagctgga
     6841 gcaacaaggt tttaccggcg atgcggcgac aagttatgcc gatcgtctga caatgtcgac
     6901 cgacaacagt atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga
     6961 tgtggcgctg atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga tctttacccc
     7021 ccgcggcggc ggcaccggca ctaacggtca ggcgctcaac caggggatta ttgttgatat
     7081 gtcccgccat atgaaccgca tcatcgaaat taaccctgaa gagggctggg tgcgcgttga
     7141 ggccggggtg ataaaagacc aactcaatca gtacctgaaa ccgttcggct acttttttgc
     7201 gccggaactt tcgaccagca accgggcaac gctcggtggg atgatcaata ccgatgcatc
     7261 cggtcaggga tcgctggtct atggcaaaac gtcagatcac gtacttggcg tacgcgcggt
     7321 gttgttgggg ggcgatattc tcgatacgca acctttaccc gtcgagctgg cggaaacgct
     7381 gggtaaatcc aataccacaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca
     7441 gcaacgccag ttaatcatcg acaacttccc caaacttaac cgctttctta ccggttacga
     7501 tctgcgtcat gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg
     7561 ttcagaaggg acgctggcct ttattaccga agcgcggctg gatattacgc gcttgcctaa
     7621 agtgcgccgt ctggtgaatg tcaaatatga ctcttttgac tctgcgctgc gtaacgcgcc
     7681 gtttatggtt gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct
     7741 ggcgcgggaa gatattgtct ggcattccgt cagcgagttg attaccgatg tgcctgacaa
     7801 agagatgctc ggactgaaca ttgtggaatt tgctggtgat gatgaggcgc tgattgacga
     7861 gcgagttaat gcactctgtg tgcggcttga tgagctgatt gctagccaac aggcaggtgt
     7921 gattggctgg caggtgtgcc gcgagctggc gggggttgaa cgtatctatg cgatgcgcaa
     7981 aaaagccgtt ggtctgcttg gcaatgccaa aggtgccgct aagccaattc cgtttgctga
     8041 ggatacctgc gtaccgccgg aacatctggc cgattatatt gctgaatttc gcgcgctgct
     8101 cgacagccac ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt
     8161 ccgtccggca ctggatatgt gcgatccgca acaagagatt ttgatgaagc aaatctctga
     8221 tgacgtggtg gcgctgactg cgaaatacgg tggtttgttg tggggcgagc acggcaaagg
     8281 ttttcgcgct gaatacagcc cggcgttttt cggtgaggaa ctttttgcag aactgcgcaa
     8341 agtgaaagcg gcatttgacc cgcataaccg actcaaccca gggaagattt gcccgccaga
     8401 aggcctcgat gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcggca
     8461 gatccccatt gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg gcaacggttt
     8521 atgcttcaac tttgatgccc gtagtccgat gtgtccgtcg atgaagatca cccagaaccg
     8581 gattcattca ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgct tgttggcgga
     8641 tcgcggcgtt gacccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg
     8701 gacgttaatt gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat atgacttctc
     8761 acacgaagtc aaagaggcga tgtcgggctg tctggcctgt aaagcttgtt cgacccagtg
     8821 ccccatcaaa attgatgtgc cggagtttcg ctctcgtttt ctgcagctct atcacacccg
     8881 ttatttacgc ccgcttcgcg accacctcgt tgctacggtc gagagctacg caccgctgat
     8941 ggcacgcgcg ccgaaaacct ttaacttctt cattaaccag ccgctggtgc gcaaactctc
     9001 ggaaaaacat atcggcatgg ttgatttgcc actgctgtcg gtcccctcgc tacaacaaca
     9061 aatggtgggg catcgctcgg caaacatgac gctggaacag cttgaagctc tcaatgcaga
     9121 gcagaaagcg cgcacggtgt tggtggtgca ggaccccttt accagctatt acgatgcgca
     9181 agtggtggcg gattttgtcc gtctggttga aaaattaggt ttccagcctg tgttactgcc
     9241 attttcgcca aatggcaaag cccagcatat caaaggcttt cttaatcgtt ttgcgaagac
     9301 ggcgaaaaag acggcggatt tcctcaaccg tatggctaag cttgggatgc caatggtggg
     9361 cgtcgatccg gcgctggtac tttgttatcg cgatgaatat aaactggcgc tgggcgagga
     9421 acgtggcgag tttaacgtct tacttgcgaa tgaatggctg gcaagcgcac ttgagtcaca
     9481 tccagtggct acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaggttac
     9541 cgctttgccg ggcgcgccag cacaatgggc cgcgatattt gcccgttttg gcgcgaaact
     9601 ggaaaatgtc agcgtgggtt gctgcggcat ggcagggact tacggacatg aagcgaaaaa
     9661 ccatgaaaat tcgctcggga tctatgagtt atcctggcat caggctatgc agcgactgcc
     9721 gcgtaaccgc tgtctggcga ccggatattc ctgccgtagc caggtaaaac gggttgaagg
     9781 cacgggggta cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacgg
     9841 aaaatcaccc tggaagcact gaatgctatg ggtgaaggaa acatggtggg gttcctggat
     9901 attcgctttg aacatattgg tgatgacacc cttgaagcga caatgccagt agactcgcgg
     9961 acaaagcagc ctttcgggtt gctgcatgga ggagcatccg tggtactggc cgaaagtatc
    10021 ggttccgttg ccggttattt atgtaccgaa ggtgagcaaa aagtggttgg tctggaaatc
    10081 aatgctaatc acgtccgctc ggcacgagaa gggcgggtgc gcggcatatg taaaccgttg
    10141 catctcggtt cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg
    10201 tgctgttcgt cacgattgac gaccgccatt ttgtgacgtt gtcaccagaa aagtgtgacg
    10261 catattgctt ttggtcatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct
    10321 aaaagcgagg atgctatgtc tactcaactt gatcccaccc agctggccat tgaattttta
    10381 cgccgtgacc agagcaatct ttcacctgcc caatatctca aacgtctgaa acaactagaa
    10441 cttgaatttg ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttt
    10501 gcctggcgtc tgggcgtgca ttgatcctga cttaatgccg caagttcgcc aaatcgacct
    10561 ggattaacct ccgtccaggt tcgatgacct tcttaatact gcttatctac cgcctgatat
    10621 tgttcaaaaa taatactaat cgcagtttgt gttaaaacgg cgggttagct ttatgagatt
    10681 attcttatcg ccccttcaag agctaagcca ctgagagtgc cggagataag cgccggatgg
    10741 ggtagaaacc cttaagcctg tgtcgcacag acttaagggt tttcttattt ctgaacgctg
    10801 cactttttta cctatctctt aaatagtcat tttcaataca actttttctt tttcatcaca
    10861 gccttaatgc tccttagata ataaccatta tctaacaatg agatacctaa ttcttagcgt
    10921 gcctgttaac ccacacatca ggggctatgc ttattaaaag caggcaaaaa gcatgtttta
    10981 cggtaaagcc cctgcgtttg ctgggttgaa ctgataatca ttatcactaa catgctgtta
    11041 tacgctgaaa gcgatgaagt gaggtaaata gatggacatg cattcaggaa cctttaaccc
    11101 acaggatttc gcctggcaag gcttaacgct gacacccgca gcggcgatac acatccgcga
    11161 gctggtggca aagcagccag gtatggtcgg cgtgcgctta ggtgtgaagc aaacgggctg
    11221 cgcgggcttt ggctatgtgc tcgacagtgt tagcgagccg gacaaagacg atctgctgtt
    11281 tgaacacgac ggcgcgaagc tgtttgtccc gctgcaatcg atgccgttta ttgatggcac
    11341 ggaaatcgat ttcgttcgtg aaggacttaa tcagatattc aaatttcaca accctaaagc
    11401 ccagaatgaa tgtggctgtg gcgaaagctt tagggtatag gcggtactat gtctcgtaat
    11461 actgaagcaa ctgacgatgt caaaacctgg accggcggcc cgctgaatta taaagaagga
    11521 ttcttcaccc agttagccac cgatgagctg gcaaagggga taaacgaaga ggtggtgcgc
    11581 gcaatttcgg cgaagcgtaa tgagccggag tggatgctgg agtttcgtct aaacgcctat
    11641 cgcgcatggc tggagatgga agaaccgcac tggttgaaag cgcactacga caagctgaat
    11701 tatcaggatt acagctacta ctcagcacca tcgtgcggta attgtgacga cacttgcgcg
    11761 tctgaacctg gcgcggtgca gcaaactggc gcgaacgcct ttttaagtaa agaggtggag
    11821 gcggcgtttg agcagttggg cgttcccgtg cgggaaggca aagaggtggc ggtggatgcc
    11881 attttcgact ctgtttcggt tgccaccact tatcgcgaaa aactggcgga gcagggaatt
    11941 attttctgtt cctttggtga ggcgatccac gatcacccgg aactggtgcg taaatatctc
    12001 ggcaccgtgg tgccggggaa tgacaacttc tttgcagcac ttaatgcggc ggtagcctct
    12061 gacggtacgt ttatttatgt gcctaaaggc gtgcgctgcc cgatggaact ttccacctat
    12121 tttcgcatta acgcggaaaa aaccgggcag tttgagcgca caattctggt ggccgacgaa
    12181 gacagctacg tcagctacat tgaaggctgt tccgctccgg tgcgtgacag ctatcagttg
    12241 cacgcggcag tggtggaagt catcatccat aaaaacgccg aggtgaaata ttccacggta
    12301 caaaactggt tccctggcga taacaacacc ggcggtattc tcaacttcgt caccaagcgt
    12361 gctttgtgcg aaggcgaaaa cagcaaaatg tcatggacgc aatcagaaac cgggtcagcg
    12421 attacgtgga aatatcccag ctgcattttg cgcggcgata actccattgg tgagttttac
    12481 tcagtggcgc tgaccagcgg tcatcagcaa gcggataccg gcaccaagat gatccacatc
    12541 ggtaaaaaca ccaaatcgac cattatctcg aaagggatct ctgccggaca tagtcagaac
    12601 agttatcgcg gcttagtgaa aatcatgccg acggcaacca atgcgcgcaa tttcactcag
    12661 tgcgactcaa tgctgattgg cgctaattgt ggggcgcata ccttcccgta tgtcgaatgt
    12721 cgcaataaca gcgcacaact ggagcacgag gcaacgacat cacgtattgg tgaagatcaa
    12781 ctgttttact gcctgcaacg cgggatcagc gaagaagacg ccatctcgat gattgttaac
    12841 ggtttctgca aagacgtgtt ctcagaactg ccgctggaat ttgccgttga agcacaaaaa
    12901 ctcctcgcca tcagtcttga acacagcgtc ggataaggaa taaacatgtt aagtattaaa
    12961 gatttacacg tcagcgtgga agataaagct atcctgcgtg gattaagcct cgacgttcgt
    13021 cccggcgaag ttcacgccat tatggggcca aacggttcgg gcaaaagtac tttatcggca
    13081 acgcttgccg ggcgcgaaga ttatgaagtg acgggcggca cggttgagtt caaaggcaaa
    13141 gatttgcttg cactgtcgcc ggaagatcgt gcaggcgaag gcatctttat ggccttccag
    13201 tatccggtgg agattccagg cgtcagtaac cagtttttcc tgcaaacggc acttaatgca
    13261 gtgcgcagtt atcgcggcca ggaaacgcac gaccgctttg attttcagga tttgatggaa
    13321 gagaaaatcg ctctcctgaa gatgccggaa gatttattaa cccgttcggt aaacgttggt
    13381 ttttccggcg gcgagaaaaa gcgcaacgat attttgcaaa tggcggtgct ggaaccggag
    13441 ttatgcattc ttgatgagtc ggactccggg ctggatattg acgcattaaa agtggtcgcc
    13501 gatggcgtga actcgctgcg tgatggcaag cgctcattca tcattgttac gcactaccaa
    13561 cgcattctcg actacatcaa gcctgattac gttcatgtgc tatatcaggg acgaattgtg
    13621 aaatccggcg atttcacgtt ggtcaaacaa ctggaggagc agggttatgg ctggcttacc
    13681 gaacagcagt aatgcgctgc aacagtggca tcacttgttt gaagctgaag ggacaaaacg
    13741 ctcaccgcaa gcacagcagc atttacaaca attgctgcgt accggactgc cgacacgtaa
    13801 acatgaaaac tggaaatata cgccgctgga agggctgacc aatagtcagt ttgtcagcat
    13861 tgcgggagat atatccccac agcagcgtga tgccttagcg ttaacgttag acgccgtgcg
    13921 gctggtgttt gtcgatggac gttacgtgcc cgcgctgagc gatgcaattg aaggcagcgg
    13981 atatgaagtg agcattaacg acgaccgtca ggggttaccc gacgctattc aggcggaagt
    14041 gtttctgcat ttgacggaaa gcctggcaca aagcgtgacg catatcgccg tgaagcgcgg
    14101 tcaacggccg gcaaagccat tgctgttaat gcatatcacc cagggcgtgg caggtgaaga
    14161 ggtgaacact gcccattacc ggcatcatct ggatctggcg gaaggtgccg aagcaacggt
    14221 gatcgaacat tttgtcagcc ttaatgatgc tcgtcatttt accggggcac ggttcactat
    14281 caatgtcgca gcgaacgccc acttgcagca tatcaagctg gcgtttgaaa acccggtcag
    14341 tcaccacttt gcccataacg atttgttgct ggctgacgat gccaccgcat ttagccatag
    14401 tttcctgctg ggtggcgcag tgttacgaca caacaccagt acgcaactca atggcgaaaa
    14461 cagcacgctg cggatcaata gcctggcgat gccggtgaaa aacgaggtgt gtgatacccg
    14521 tacctggctg gaacacaata aaggtttttg taacagccga cagttgcaca aaactatcgt
    14581 cagcgacaaa ggccgcgcgg tatttaacgg tttgatcaac gtcgcgcagc acgccatcaa
    14641 aacggatggt cagatgacca acaataatct gctgatgggc aaactggcgg aagtggatac
    14701 aaaaccgcag ctggaaatct atgcagatga tgtgaaatgc agccacggcg cgacggtggg
    14761 gcgtattgat gatgaacaga tgttctatct gcgctcgcgc gggatcaatc agcaggatgc
    14821 ccagcagatg atcatttacg cctttgctgc cgaactgacg gaagcactgc gtgatgaggg
    14881 gtttaaacag caggtgctgg cccgaatcgg tcaacggctg ccaggaggtg caagatgact
    14941 ttttccgtcg acaaagtgcg ggccgacttt ccggtgcttt cgcgtgaggt aaacggtttg
    15001 ccgctggctt atctcgacag cgccgccagt gcacagaaac cgagccaggt gattgacgcc
    15061 gaggccgagt tttatcgtca tggctacgcg gcggtgcatc gcggtattca taccttaagc
    15121 gcccaggcga ccgagaaaat ggagaacgtg cgcaagcggg catcgctgtt tattaatgcc
    15181 cgttcggcgg aagagctggt gttcgtccgc ggcacgacgg aagggatcaa tctggtcgcc
    15241 aatagctggg gcaacagcaa tgtgcgggcg ggcgataaca tcatcatcag tcagatggag
    15301 caccacgcta acattgtccc ctggcagatg ctttgcgcac gcgttggcgc agagctgcgt
    15361 gtgatccctc tcaaccccga cggtacgctg caactggaga cgctgcctac gctgtttgat
    15421 gagaaaactc gcctgctggc aattactcat gtctccaacg tgcttggcac agaaaatcca
    15481 ctggcggaaa tgatcacgct tgcgcaccag catggcgcaa aagtgctggt ggatggcgct
    15541 caggcggtga tgcatcatcc ggtggatgtt caggcgctgg gttgcgactt ttacgtgttc
    15601 tccgggcata aactgtatgg ccccaccgga attggcattc tttatgtgaa agaagccttg
    15661 ttgcaggaga tgccgccgtg ggaagggggc ggttctatga tcgccaccgt cagcctgagt
    15721 gaaggcacta cctggaccaa agcaccatgg cggtttgaag ccggtacacc caataccggg
    15781 ggcatcattg gtcttggcgc ggcgctggag tatgtttcgg cgctggggct taatagcata
    15841 gccgagtatg aacagaatct gatgcattat gcgctatcac agctggaatc tgtaccggat
    15901 ctcactctct atggccaaca aaacaggctt ggcgttattg cttttaatct cggtaaacac
    15961 cacgcctatg atgttggcag ttttctcgat aattacggca ttgctgtgcg taccggacat
    16021 cactgcgcta tgccattaat ggcctattac aacgtccctg cgatgtgtcg ggcgtcgctg
    16081 gccatgtata acacccatga agaagtggat cgtctggtga ccggcctgca gcgtattcac
    16141 cgtctgctgg gataacaggg aggcactatg gctttattgc cggataaaga aaaattgctg
    16201 cgtaattttt tacgctgcgc caactgggaa gagaaatatc tctacattat tgagctgggc
    16261 cagcgtctgc cagaattacg cgccgaagac ggaagcccac aaaatagcat tcagggctgc
    16321 cagagtcagg tgtggattgt catgcgccag aatgcgcagg gaattattga attgcagggc
    16381 gacagcgatg cggcgattgt gaaagggctt attgcggtcg tctttattct ctacgatcag
    16441 atgacgccgc aggatattgt caatttcgat gtgcgtccgt ggtttgaaaa aatggcgctc
    16501 acccaacatc tcaccccatc tcgttcacaa ggtctggaag cgatgattcg cgcaattcgc
    16561 gccaaagccg ctgcacttag ctaaactaaa gggacagctt tcatccggtt ggctcacatt
    16621 gccggatggt caatgtttac actgacctgc acaggagtgt tttggtttca ggtgaacata
    16681 agcaccatcc gacgtttcag cgtgagtctc cggcaaatac aggaggttta caatgaaacg
    16741 cgcgtctttg cttacactca cgcttatcgg cgcttttagc gccatccagg ctgcctgggc
    16801 ggttgattat ccgctaccac caaccggaag ccgactggtt gggcaaaatc aaacgtatac
    16861 ggtgcaagaa ggggataaaa accttcaggc tattgcccga cgttttgata ctgcggcaat
    16921 gttgatcctt gaagccaata acactatcgc cccggtgcca aaacctggta cgacgataac
    16981 tattccttca caactgttat tacctgatgc accgcgtcag gggattatcg ttaaccttgc
    17041 agagctgcgc ctttattatt atccgccggg agaaaatatt gtgcaggttt atccaatagg
    17101 tattggattg caggggctgg aaacgccggt gatggaaacg cgtgttgggc agaaaatccc
    17161 taacccaacc tggacgccta cggcaggcat tcgtcagcgt tcgctggagc gtggcattaa
    17221 attaccgcca gtcgttcctg ccggaccaaa taacccgcta ggacgttacg cactgcgcct
    17281 cgcgcatggt aatggcgaat acctcattca tggcaccagt gcgccggaca gcgttggttt
    17341 gcgcgtcagt tcagggtgta ttcgcatgaa tgcaccggat attaaagcct tgttctccag
    17401 cgtgcggacg ggaacgccgg tgaaagtgat caacgaaccg gtgaaatatt ccgtggaacc
    17461 taacgggatg cgttatgttg aagtacatcg accactatca gcagaagaac agcaaaacgt
    17521 tcagacaatg ccatacacac tgccagcagg ctttacgcaa tttaaagaca ataaggctgt
    17581 agatcagaag ttagtcgata aagcgttgta tcgtcgggca gggtatccgg tttcggtgag
    17641 cagtggagca actcccgcag ccagcaatgc gccttcagta gagtcagcgc agaatggtga
    17701 accagagcaa gggaatatgt tacgcgcgac gcagtagtgg taaatagcag acaaaaaaat
    17761 ggcgcacaat gtgcgccatt tttcacttca caggtactat tacttgcggt atttagtagc
    17821 catgttgtcc agacgctggt tagcacgagc tgcgtcatct ttagcagcct gaacgtcgga
    17881 acgcattgcg ttcacgtcgt tgctcagctg gtcaacttta gcgttcagag tctgaacgtc
    17941 agaagacagc tgatcgattt tagcgttgct ggagcaacct gccagcagag tagaacccag
    18001 gattaccgcg cccagtacca gtttagtagc tttcattatt aataccctct agattgagtt
    18061 aatctccatg tagcgttaca agtattacac aaagtttttt atgttgagaa tatttttttg
    18121 atgggaaggc acttattttt gatcgttcgc tcaaagaagc atcgaacgct gggttttaga
    18181 taaaaaaatt gagagaaaac aggatgcttc catcggattc atcttaaata aagtgagatt
    18241 atatagtgaa atccgattt
//
LOCUS       DADMEQ010000073        18173 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.073, whole genome shotgun sequence.
ACCESSION   DADMEQ010000073 DADMEQ010000000
VERSION     DADMEQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.073"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            1..447
                     /locus_tag="JNZ05_002559"
                     /pseudo
     CDS             1..447
                     /locus_tag="JNZ05_002559"
                     /inference="COORDINATES: protein motif:HMM:NF013106.1"
                     /note="penicillin-insensitive
                     transglycosylase/transpeptidase; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
     gene            934..2083
                     /locus_tag="JNZ05_002560"
                     /pseudo
     CDS             934..2083
                     /locus_tag="JNZ05_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
     gene            2233..2664
                     /gene="ndk"
                     /locus_tag="JNZ05_002561"
     CDS             2233..2664
                     /gene="ndk"
                     /locus_tag="JNZ05_002561"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAY8565749.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2814..3968
                     /gene="trmG/rlmN"
                     /locus_tag="JNZ05_002562"
     CDS             2814..3968
                     /gene="trmG/rlmN"
                     /locus_tag="JNZ05_002562"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HAY8565750.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4253..5266
                     /gene="rodZ"
                     /locus_tag="JNZ05_002563"
     CDS             4253..5266
                     /gene="rodZ"
                     /locus_tag="JNZ05_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HAY8565751.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5293..6411
                     /gene="ispG"
                     /locus_tag="JNZ05_002564"
                     /gene_synonym="gcpE"
     CDS             5293..6411
                     /gene="ispG"
                     /locus_tag="JNZ05_002564"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HAY8565752.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            6522..7796
                     /gene="hisS"
                     /locus_tag="JNZ05_002565"
     CDS             6522..7796
                     /gene="hisS"
                     /locus_tag="JNZ05_002565"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAY8565753.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7814..8434
                     /locus_tag="JNZ05_002566"
     CDS             7814..8434
                     /locus_tag="JNZ05_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HAY8565754.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8445..9623
                     /gene="bamB"
                     /locus_tag="JNZ05_002567"
     CDS             8445..9623
                     /gene="bamB"
                     /locus_tag="JNZ05_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HAY8565755.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTTVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9741..11213
                     /gene="der"
                     /locus_tag="JNZ05_002568"
     CDS             9741..11213
                     /gene="der"
                     /locus_tag="JNZ05_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAY8565756.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11283..11498
                     /locus_tag="JNZ05_002569"
     CDS             11283..11498
                     /locus_tag="JNZ05_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565757.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11495..12865)
                     /gene="xseA"
                     /locus_tag="JNZ05_002570"
     CDS             complement(11495..12865)
                     /gene="xseA"
                     /locus_tag="JNZ05_002570"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAY8565758.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWFFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13027..14493
                     /gene="guaB"
                     /locus_tag="JNZ05_002571"
     CDS             13027..14493
                     /gene="guaB"
                     /locus_tag="JNZ05_002571"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAY8565759.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14562..16139
                     /gene="guaA"
                     /locus_tag="JNZ05_002572"
     CDS             14562..16139
                     /gene="guaA"
                     /locus_tag="JNZ05_002572"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAY8565760.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEAQKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKTDLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16234..16773)
                     /locus_tag="JNZ05_002573"
     CDS             complement(16234..16773)
                     /locus_tag="JNZ05_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565761.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16789..17307)
                     /locus_tag="JNZ05_002574"
     CDS             complement(16789..17307)
                     /locus_tag="JNZ05_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY8565762.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17618..17809)
                     /locus_tag="JNZ05_002575"
     CDS             complement(17618..17809)
                     /locus_tag="JNZ05_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HAY8565763.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWLHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17827..17979
                     /locus_tag="JNZ05_002576"
     CDS             17827..17979
                     /locus_tag="JNZ05_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565764.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
BASE COUNT         4300 a         4450 c         4994 g         4429 t
ORIGIN      
        1 gaagcgctga tcaattatga agatcgctgg ttctggaagc atccgggggt taatccattc
       61 tcggtggcgc gcgcagcatg gcaagatctc acttcgggac gggttatttc cggtggcagc
      121 acgctcacta tgcaggttgc tcgtctgctt gatcctcacc ccaaaacatt tggtggcaaa
      181 attctccagc tctggcgcgc gttgcaactg gagtggcatt tgtctaagcg tgaaattctg
      241 atcttgtatc ttaaccgcgc tccgtttggc ggtacgttgc aggggatcgg tacggcaagt
      301 tgggcttatc tcggaaaatc gcctgcgaat ttaagctatt ccgaggcggc aatgctggcg
      361 gtgttaccgc aagtgctcag ccgactgcgc ccggatcgtt ggccggagcg tgccgaagcc
      421 gcgcgtaata aagtgctcga acggatggct gcgcaaggtg tgtggtcccg tgagcaggta
      481 aaagagttaa gggaagaacc catctggctg gcaccccgac aaatgccgca actggcaccg
      541 ctgttttcgc gcatgatgct cggtaaaagc aaaagcgaca aaatcgttac tacgctggat
      601 gccggtcttc aacgacgtct ggaagaactg gcgcaaaact ggaaagggcg gttgccaccg
      661 cgcagctcac tggcgatgat cgtggttaat cataccgata tgcgtgttcg cggctgggtg
      721 ggatcggttg atctcaacga tgattcacgc tttggtcatg ttgatatggt caatgcgatc
      781 cgatcgccag gatcggtgct caaaccgttt gtttatggtc tggcgctgga tgaaggcttg
      841 atccacccgg catcactgct gcaagacgtc ccccggcgca ccggtgatta tcgaccaggt
      901 aactttgata gcggttttca tggcccgatc aacatgagcg aggcgctggt gcgctcgctg
      961 aacttaccag ctgtgcaggt gctggaagcc tatggaccga aacggtttgc ggcaaagtta
     1021 cgcaatgttg gattgccgtt atatttgccc aacggcgctg cgccgaatct ttcactcatt
     1081 ctcggcggcg ctggtgcaaa actggaagat atggcggcag cgtataccgc gtttgctcgc
     1141 cacggcaagg caggcaaatt gcgcttacag cctgacgatc cgctgcttga acggccttta
     1201 atgtcgtcgg gggcggcgtg gatcattcgc cggattatgg ctgatgaagc gcaacccttg
     1261 ccggatagtg ccttgccgcg cgtcgcccca ctggcatgga aaacgggcac cagctatggc
     1321 tatcgtgacg cctgggcgat tggggttaac gctcgctatg tcattgggat ctggactggc
     1381 agacctgacg gcacgcccgt tgttggtcag tttggctttg ccagtgccgt accattagtt
     1441 gaatcaggtc aataatatct tactgtcgcg cagtgcgaat ctgccggaag acccgcgccc
     1501 gaactcagtc actcgtggcg ttatctgctg gccgggcgga cagtctttgc cggaaggtga
     1561 cggtaactgt cgccgccgcc tggcaacctg gctgctggat ggcagccagc cgccaacgct
     1621 gttactgtca gagcaggaag ggattaatgg cattcgcttc cccatctggc tggatgacaa
     1681 agggcagcgc gtcgccgctg acttcccgca ggcgaagcaa gaaatggtaa atgtctggcc
     1741 gctaccgctg gaaccctggc tgcccgcatc agaacgtcgc gctgtgcgct taccaccggc
     1801 ctcgacaatt tgcccgccat acggtcacga tgcccagctc cctctgcagc tgacaggcgt
     1861 tcgcgatggc gcgattatta aacgtttacc gggcgcagcg gaagcgactt tgccgttgca
     1921 atcaagtgga ggggcaggtg aacgctggtg gtttctgaat ggcgaaccgt taactgaacg
     1981 cgggcgcaac gtgaccctgc atttgacgga taaaggtgat tatcaattgc tggtgatgga
     2041 tgatgtgggg caaatcgcga cagtgaaatt tgtcatgcaa tagtctgttt tgtccctgat
     2101 tgttgctaaa actcatttta ttttaaaaaa aatgttacct tcatcaatag tcaacggccc
     2161 tgttgctcat tataatccgc gccatctcgt acgctggtac agacaacaac agaacaattt
     2221 acagaggtaa aaatggctat tgaacgtact ttttccatca tcaaaccgaa cgcggtagca
     2281 aaaaacgtca ttggtaatat ctttgcgcgc tttgaagctg cagggttcaa aattgtcggc
     2341 accaaaatgc tgcacctgac cgttgaacag gcacgtggct tttatgctga acacgatgga
     2401 aaaccgttct ttgatggtct ggttgaattc atgacctctg gcccgatcgt ggtttccgtg
     2461 ctggaaggtg aaaacgccgt tcagcgtcac cgcgatctgc tgggcgcgac caatccggca
     2521 aacgcactgg ctggtactct gcgcgctgat tacgctgaca gcctgaccga aaacggtacc
     2581 cacggttctg attccgtcga atctgccgct cgcgaaatcg cttatttctt cggcgaaggc
     2641 gaagtgtgcc cgcgcacccg ttaataattt cgtaaatgcc gcgtgcaaac gtggcatccg
     2701 tgcgccagaa tttgtacaat gcagcgcccc cggacgagca gccgctcacc ggggcgtttc
     2761 ttttttcaac cctccagggg ccataacgtg taataacgag gccggaatag catatgtctg
     2821 aacaattagt cacacctgaa aacgtcacca cgaaagatgg aaaaatcaac ctgctggatc
     2881 tcaaccgtca gcagatgcgg gagtttttta aagatttagg tgaaaaaacc ttccgcgccg
     2941 atcaggtgat gaagtggatg tatcactatt gctgcgacaa ctttgatgag atgaccgaca
     3001 tcaacaaagt gttgcgcggc aaactgaaag aggtggcgga aatccgcgca ccggaagtgg
     3061 ttgaagaaca gcgttcatcc gacggcacca ttaaatgggc gatcgccgtt ggcgatcagc
     3121 gcgtcgaaac ggtgtatatc ccggaagacg accgtgccac gctctgcgtc tcttcgcagg
     3181 tggggtgtgc gctggagtgt aaattctgtt ccaccgccca gcagggcttt aaccgcaacc
     3241 tgcgggtatc ggaaattatt ggccaggtgt ggcgtgcggc gaaaatcgtc ggcgcagcaa
     3301 aagtcaccgg tcagcgccca atcactaacg tcgtgatgat gggcatgggc gagccgctgc
     3361 tcaacctgaa caacgtcgtt ccggcgatgg aaatcatgct tgatgatttc ggttttggcc
     3421 tgtctaaacg tcgcgtgacg ctttccactt ccggtgtagt tccggcgctg gataaactgg
     3481 gcgatatgat cgacgttgca ctggcaattt ccctgcacgc gccgaacgac gaaattcgtg
     3541 acgaaattgt gccgatcaac aaaaagtaca atatcgaaac gttccttgct gcggtgcgcc
     3601 gttatctgga gaaatccaac gccaatcagg gccgagtcac tattgaatac gtgatgcttg
     3661 accacgttaa cgacggcact gaacacgcgc accaactggc ggaactgctg aaagatacgc
     3721 cgtgtaagat caacctgatc ccgtggaacc cgttcccggg cgcgccgtat ggacgcagtt
     3781 cgaacagccg tatcgaccgc ttctcaaaag tgctgatgag ctacggtttc accactattg
     3841 tgcgtaaaac gcgtggtgat gatatcgatg ctgcctgtgg tcagttggcg ggcgatgtta
     3901 tcgaccgtac gaaacgtact ctgcgtaaac ggatgcaggg tgaagccatc gatattaaag
     3961 cggtctgata gccattacgc cacggttact ttatgtaccg tggcgtaatc ttttatgaat
     4021 gaagtattaa gttgcaattg tctggtcaat attaacggtg cgtttttgct gactttaagg
     4081 cagtatgtaa cgatgcaaca gtaagttagc ctcagtgaat gtgggctttg tcacgagcac
     4141 acagacggtc ttatactgta tgataacggt taacttaacg gatgtttcgc ggtgtgggtg
     4201 agcatgatgg ttcaccggca tctcaattct catttaaacg tacctgcagc gaatgaatac
     4261 tgaagccacg cacgaccaaa atgaagcact tactaccggc gctcgcctgc gtaatgctcg
     4321 cgaacaacta ggacttagtc agcaggccgt tgccgagcga ctttgcctga aggtttccac
     4381 ggtacgcgac attgaagaag ataaggcacc cgccgatctt gcttcaacat tcctgcgcgg
     4441 atatatccgc tcttatgcgc gtctggtaca tattccagaa gaagaactgc tgccagggct
     4501 ggaaaagcag gctccacttc gggctgcaaa agttgcgcca atgcagagtt tttccctcgg
     4561 taaacgccgc aaaaaacgcg acggctggct gatgaccttc acctggctgg tgttgtttgt
     4621 ggttatcggc ctgagcggtg cctggtggtg gcaagaccac aaagctcagc aggaagagat
     4681 caccactatg gccgatcaat cttcggcgga actgagcagt aatagcgagc aggggcagag
     4741 tgttccgtta aatacgtcga caactacaga cccggctaca acgtccacgc cgccagcgtc
     4801 tgtggatact accgcaacca acacgcaaac acctgccgta actgcgccag caccagctgt
     4861 tgatccgcaa cagaatgcgg ttgtttcgcc ttcgcaggca aatgttgata ccgccgcgac
     4921 cccggtacca acggcagcaa caacgccaga tggtgctgcg ccgttgccaa ccgatcaggc
     4981 tggcgtgacc acgccggcgg ctgatccgaa tgcgctggtg atgaacttta ctgccgattg
     5041 ctggctggag gtcactgatg ctaccggtaa aaaattgttt agcgggatgc agcgtaaaga
     5101 cggtaatttg aatttaaccg gccaggcacc gtataaactg aaaattggtg cgccagccgc
     5161 agtacagatc cagtatcaag ggaaacctgt cgatctgagt cgttttatca gaactaacca
     5221 ggttgcgcgt ctgaccctca atgccgaaca atcaccggcg cagtaacaga cgggtaacgc
     5281 gggagatttt tcatgcataa ccaggctcca attcaacgta gaaaatcaac acgtatttac
     5341 gttgggaatg tgccgattgg cgatggtgct cccatcgccg tacagtccat gaccaatacg
     5401 cgtacgacag atgtcgaagc aacggtcaat caaatcaagg cgctggaacg cgttggcgct
     5461 gatatcgtcc gtgtctccgt accgacgatg gacgcggcag aagcgttcaa actcatcaaa
     5521 cagcgggtta acgtgccgct ggtggctgac atccacttcg actatcgcat tgcgctgaaa
     5581 gtagcggaat acggcgtcga ttgtctgcgt attaaccctg gtaatatcgg taatgaagag
     5641 cgtattcgca tggtggttga ctgtgcgcgc gataaaaaca ttccgatccg tattggcgtt
     5701 aacgccggat cgctggaaaa agatctgcaa gaaaagtatg gcgaaccgac gccgcaggcg
     5761 ttgctggaat ccgccatgcg tcatgttgat catctcgatc gcctgaactt cgatcagttc
     5821 aaagtcagcg tgaaagcgtc tgacgtcttc ctcgctgttg agtcttatcg tttgctggca
     5881 aaacagatcg atcagccgct gcatctgggg atcaccgaag ccggtggtgc acgcagcggg
     5941 gcagtaaaat ccgccattgg tttaggtctg ctgctgtcgg aaggcatcgg cgacacgctg
     6001 cgcgtatcgc tggcggccga tccggtcgaa gagatcaaag tcggtttcga tattttgaaa
     6061 tcgctgcgta tccgttcgcg agggattaac ttcatcgcct gcccgacctg ttcgcgtcag
     6121 gaatttgatg ttatcggtac ggttaacgcg ctggagcaac gcctggaaga tatcatcact
     6181 ccgatggacg tttcgattat cggctgcgtg gtgaatggcc caggtgaggc gctggtttct
     6241 acactcggcg tcaccggcgg caacaagaaa agcggcctct atgaagatgg cgtgcgcaaa
     6301 gaccgtctgg acaacaacga tatgatcgac cagctggaag cacgcattcg tgcgaaagcc
     6361 agtcagctgg acgaagcgcg tcgaattgac gttcagcagg ttgaaaaata ataacgtgat
     6421 gggaagcgcc tcgcttcccg tgtatgattg aacccgcatg gctcccgaaa cattgaggga
     6481 agcgttgagg gttcattttt atattcagaa agagaataaa cgtggcaaaa aacattcaag
     6541 ccattcgcgg catgaacgat tacctgcctg gcgaaacggc catctggcag cgcattgaag
     6601 gcacactgaa aaacgtgctc ggcagctacg gttacagtga aatccgcttg ccgattgtag
     6661 agcagacccc gctattcaaa cgtgcgattg gtgaagtcac cgacgtggtt gaaaaagaga
     6721 tgtacacctt tgaggatcgc aatggcgaca gcctgactct gcgccctgaa gggacggcgg
     6781 gctgtgtacg cgccggcatc gagcatggtc ttctgtacaa tcaggaacag cgtctgtggt
     6841 atatcgggcc gatgttccgt cacgagcgtc cgcagaaagg gcgttatcgt cagttccatc
     6901 agttgggctg cgaagttttc ggtctgcaag gtccggatat cgacgctgaa ctgattatgc
     6961 tcactgcccg ctggtggcgt gcgctgggta tcttcgaaca cgtaactctt gagctgaatt
     7021 ctatcggttc gctggaagca cgcgccaatt accgcgatgc gctggtggca ttccttgagc
     7081 agcataaaga aaagctggac gaagactgca aacgccgcat gtacactaac ccgctgcgcg
     7141 tgctggattc aaaaaatccg gaagtgcagg cgcttctcaa cgacgctccg gcattaggtg
     7201 attatctgga cgaggaatct cgtgagcatt ttgccggtct gtgcaaactg ctggagagcg
     7261 cggggatcgc ttacaccgta aaccagcgtc tggtgcgtgg tctggattac tataaccgta
     7321 ccgttttcga gtgggtgact aacagtctcg gctcccaggg caccgtgtgt gcaggcggtc
     7381 gttatgacgg tcttgtggaa caactgggcg gtcgtgcaac accggctgtc ggttttgcga
     7441 tgggcctcga acgtcttgta ttgttagtac aggccgttaa tccggaattt aaagccgatc
     7501 ctgttgtcga tatatacctg gtggcttcag gtgctgatac acaatctgcg gctatggcat
     7561 tagctgagcg tctgcgtgat gaattactgg gcgtgaaatt gatgaccaac cacggcggcg
     7621 gcaactttaa gaaacagttt gcccgtgctg ataaatgggg tgcccgcgtt gctgtggtgc
     7681 tgggtgagtc tgaagtggct aacggcacag cagtagtgaa ggatttgcgc tctggtgagc
     7741 aaacggcagt tgcgcaggat agcgtagccg cgcatttgcg cacgttactg ggttaaggaa
     7801 ggagaaggac agcgtggaaa tttacgagaa cgaaaacgac caggtagaag cggttaaacg
     7861 cttttttgct gaaaatggca aagcactggc tgttggggtg attttgggcg ttggcgcact
     7921 gattggctgg cgctactgga acagccatca ggttgattct gcacgctccg cttctcttgc
     7981 ctatcaaaat gcggttaccg cagtgagcga aggcaaaccg gatagcatcc cggcggcgga
     8041 aaaatttgct gctgaaaata aaaatactta tggtgcgctg gcttctttgg aacttgcgca
     8101 gcaatttgtt gacaaaaatg aactagagaa agctgccgcc cagttacaac aggggctggc
     8161 agacacgagc gatgaaaatc tcaaagccgt gataaatctg cgtcttgctc gcgttcaggt
     8221 acagctcaag caggctgatg ccgcgctgaa aacccttgat accatcaaag gtgaagggtg
     8281 ggctgccatt gttgccgacc tgcgtggtga agcattgctg agcaaaggtg ataagcaagg
     8341 tgcgcgtagt gcatgggaag caggcgtgaa aagcgatgtt actccggcac tgagcgaaat
     8401 gatgcagatg aaaattaata atttgtccat ctgagaggga cccgatgcaa ttgcgtaaat
     8461 tactgctgcc aggactgctt tccgttaccc ttttaagcgg ctgttcgctg tttaacagcg
     8521 aagaagatgt ggtaaagatg tccccattgc caaccgttga aaaccagttt acgccgacca
     8581 cggcgtggag cacttccgtt ggtagcggca ttggcaactt ctattccaat cttcatccgg
     8641 cactggcgga caacgttgtc tatgcagcgg accgcgctgg tttggtaaaa gcgctgaatg
     8701 cggatgatgg caaagaaatc tggtctgtca gcctggccga gaaagatggc tggttctcta
     8761 aagagcctgc attactttct ggcggtgtga ccgtgtctgg tgggcatgtc tacattggca
     8821 gcgaaaaggc gcaggtttac gcgctgaata ccagcgatgg tactgtggca tggcaaacta
     8881 cagtcgcggg tgaagcactt tcgcgcccgg tggtcagcga cggtctggtg ttaatccaca
     8941 ccagtaacgg tcagttacaa gcgctgaacg aagctgacgg cgctgtcaaa tggacagtta
     9001 acctcgatat gccttcgctc tctttgcgtg gcgagtctgc gccggcaacg gcttttggtg
     9061 cggccgtcgt gggtggcgat aatggtcgcg tcagcgcagt gctgatggaa cagggccaga
     9121 tgatttggca gcagcgtatt tcccaggcga ccggttctac cgaaattgac cgtctgagcg
     9181 atgttgacac gactcccgtc gttgttaacg gcgttgtttt cgcgctggcc tataatggta
     9241 acctgacggc gcttgatctg cgcagtggtc agattatgtg gaaacgcgaa ctgggttcgg
     9301 tgaatgattt catcgtcgat ggcaatcgca tctatctggt cgatcaaaat gaccgggtga
     9361 tggcgttgac cattgatggc ggcgttacgc tgtgggcaca aagcgatctg ctgcatcgcc
     9421 tgctgacttc tccggtgctg tataatggca acctggtggt cggtgacagt gaaggttatc
     9481 tgcactggat taacgtcgaa gatggtcgtt tcgttgccca gcaaaaagtt gatagttccg
     9541 gtttccagac tgaaccggtt gccgctgacg gcaaactgct gatccaggca aaagacggaa
     9601 ccgtgtactc tattacacgt taatcgtctc tgtcgttcac tttgaaaacg gctcctggac
     9661 aggggccgtt ttcctgtttt taacaacgac gcgaatatag cgtgcgttgt ctgatgattt
     9721 ataaaaatga ggctttaaac atggtacctg tggtcgcgct tgtcgggcgc cctaacgtag
     9781 gaaaatccac gttatttaac cgtctaactc gcacccgaga tgcgctggtt gcggatttcc
     9841 cgggtctgac tcgtgaccgt aagtacggtc gtgcggaaat tgaaggccgt gagtttatct
     9901 gtattgatac cggcgggatt gatggcacag aagacggtgt agaaacccgc atggcggaac
     9961 agtcgctgct ggcgattgaa gaagcggacg tcgtactgtt tatggtggat gcgcgcgcgg
    10021 gcctgatgcc ggcagatgaa gcgattgcca aacatctgcg ctcccgtgaa aaaccgacct
    10081 tcctggtggc aaacaaaact gacggtctgg atcccgatca ggcagtggtt gatttctact
    10141 cgcttggttt aggtgaaatc tacccgatcg ccgcgtctca cggtcgtggc gtattaagtc
    10201 tgctggagca tgtgctgctg ccgtggatgg aagatctcgc accgcaagag gaagtcgacg
    10261 aagacgctga atactgggcg caatttgaag cggaagagaa cggcgaagaa gaagaggaag
    10321 acgacttcga cccgcaaagt ctgccgatca aactggcgat tgtgggtcgt ccgaacgtag
    10381 gtaagtctac actcactaac cgtattcttg gagaagagcg cgttgttgtt tacgacatgc
    10441 ctggcacgac gcgtgacagc atctacatcc caatggaacg cgatggacgt gagtatgtgc
    10501 tcattgacac cgctggcgta cgtaaacgcg gcaaaatcac cgatgctgta gagaaattct
    10561 ccgtaatcaa aacgttgcag gccattgaag acgccaacgt ggtgatgtta gtgattgatg
    10621 cgcgcgaagg tatttccgat caggatctct cgctgctggg ctttattctc aatagtgggc
    10681 gctcacttgt cattgtggtg aataagtggg atggcctgag ccaggaagtg aaagagcagg
    10741 tgaaagaaac gctggacttc cgtctgggct ttatcgattt tgctcgtgtg cactttatct
    10801 ctgccttgca cggcagtggt gttggtaact tgtttgaatc agtacgtgaa gcgtatgaca
    10861 gctccacccg tcgtgtgggg acctctatgc tgacgcgcat catgacgatg gctgttgaag
    10921 atcaccaacc gccgctggta cgcggtcgtc gtgtgaagct gaaatatgcc cacgctggtg
    10981 gttataatcc gccgattgtg gtgattcacg gtaatcaggt gaaagacctg cctgattcct
    11041 acaagcgcta cttgatgaac tacttccgca aatcgctgga cgtaatggga tcgccgattc
    11101 gtattcagtt caaagaaggg gaaaacccgt atgcgaacaa gcgtaacacc ctgacgccaa
    11161 cccagatgcg taaacgtaag cgtctgatga agcacatcaa gaaaaataaa taattatccg
    11221 taagtatttt atctgccatt ccctctatga atgtagaggg aatggctacc ggaggaattc
    11281 atatggaact gcattgtcca aaatgtcagc atgtgcttga tcaggataac ggccatgcgc
    11341 gttgcccatc ctgcggggaa tttattgaaa tgaaagctct ttgtccagac tgccatcaac
    11401 cgttgcaggt gttaaaagcc tgcggtgcag tagattactt ctgtcagcac gggcatggct
    11461 tgatatcgaa aaaacgcgtt gaattcgtgc tggcttaatg aaccttttta cgcgattttt
    11521 ttaccggctg gatgtttttt acttcacttt ctatccagcc gtcttccaga cgtgtggtta
    11581 gcatttcacc cgctttaact tgcttcactt ttttcagtac attgccgtca gtagcagtag
    11641 taacgctgta tccacgcgcc agcgttgaca gtgggcttac ggcttcgagg tgcgttactg
    11701 cattaccgaa acgttcacgc gtggcgctaa gctgtgcgcg caggatttct gctaaacgat
    11761 attccagttg ctgaatgcgc gtttgcgtgc gatgaatctt cggctgtgga ttttgctgat
    11821 tcagccgctg tgttaatcgc tgctgctgtt gcccggcacg cttaagctga ttttccagcg
    11881 caaagctcat ccgtttttgc aggcgttcaa gcatggtttg ctggcgtgcc agccggagct
    11941 gcggatgctg ctgctgcaag cgatgatgaa tctgcgtaaa gcgacgcgta cggttggcga
    12001 gataataatc catcgccatc tccagccgtt gacgggccga ttgcacctgg cgcagtaact
    12061 cttgctgatt acggctcact acttcagcgg cggcagacgg cgttggcgca cgcagatcgg
    12121 caacaaaatc ggcaatggtc acatccgtct catgcccgac ggcgctgacg accggaatgc
    12181 ggctggcaaa aatcgcccgc gctacgcgtt cgtcgttaaa actccataaa tcttccagcg
    12241 aaccgccgcc gcgcccaacg atcaacacgt cgcactcatt gcgctgattc gccagttcaa
    12301 tggcgcgaac aatttgcccc ggcgcgtcat cgccctgaac ggaggtgggg tagatgatca
    12361 ccggtagaga aggatcgcga cgttttaaca catgcaaaat atcatgtagc gcagcaccgg
    12421 tttttgaggt gatcacacca acgcaatgcg caggggaggg aagtggtttt ttgtattgct
    12481 gatcgaacaa accttcagcc tgcaacttcg ctttgagctg ttcgtacttc tgttgcagca
    12541 gcccttcacc ggccggctgc atactctcaa cgattatctg gtagtcgccg cgcggctcgt
    12601 agagcgtaat attggcgcga actaaaactt gttgcccatg ctgcggacgg aaggtgaccc
    12661 ggcggttgct gttgcggaac atcgcgcagc gtacctgggc ggtgtcgtct ttgagtgtaa
    12721 agaaccagtg accggaagcc ggttgtgtga aattagagat ttcgccgctg atccaaacct
    12781 gtcccatctc atgctcaagc agcagacgaa ccgtttgatt caggcgacta acggtaaaaa
    12841 ttgcagggga ttgagaaggt aacatgtgag cgagatcaaa ttctaaatca gcaggttatt
    12901 cagtcgatag taacccgccc ttcggggata gcaagcattt tttgcaaaaa ggggtagatg
    12961 caatcggtta cgctctgtat aatgccgcgg caatatttat taaccactct ggtcgagata
    13021 ttgcccatgc tacgtatcgc taaagaagct ctgacgtttg acgacgttct cctcgttcct
    13081 gctcattcta ccgttctgcc gaatactgct gacctcagca cccagctgac gaaaactatt
    13141 cgtctgaata tccctatgct ttccgcagca atggataccg taacggaagc gcgcctggct
    13201 attgctctgg ctcaggaagg cggtatcggc tttatccaca aaaacatgtc cattgaacgc
    13261 caggcagaag aagttcgccg tgtgaaaaaa cacgaatctg gtgtggtgac tgatccgcag
    13321 actgttctgc caaccacgac gctgcgcgaa gtgaaagaac tgaccgagcg taacggtttt
    13381 gcgggctatc cggtcgttac cgaagaaaac gaactggtgg gtattatcac cggtcgtgac
    13441 gtgcgttttg ttaccgacct gaaccagccg gttagcgttt acatgacgcc gaaagagcgt
    13501 ctggtcaccg tgcgtgaagg tgaagcccgt gaagtggtgc tggcaaaaat gcacgaaaaa
    13561 cgcgttgaaa aagcgctggt ggttgatgac gaattccacc tgatcggcat gatcaccgtg
    13621 aaagacttcc agaaagcgga acgtaaaccg aacgcctgta aagacgagca aggccgtctg
    13681 cgtgttggtg ctgcagttgg cgcaggtgcg ggtaacgaag agcgtgttga cgcgctggtt
    13741 gccgctggcg ttgacgttct gctgatcgac tcctcccacg gtcactcaga aggcgttttg
    13801 cagcgtatcc gtgaaacccg tgctaaatat ccggatctgc aaatcatcgg cggcaacgtg
    13861 gcaacagctg caggtgcacg cgctttggca gaagctggtt gcagcgcggt taaagtcggt
    13921 atcggccctg gttctatctg tacgactcgt atcgttactg gcgtaggtgt tccgcagatc
    13981 actgccgttg ctgacgcagt tgaagccctg gaaggcaccg gtattccggt tatcgcagat
    14041 ggtggtattc gcttctccgg cgacatcgcc aaagctatcg ccgctggcgc aagcgcggtg
    14101 atggtaggtt ccatgctggc gggtactgaa gaatctccgg gtgaaatcga actctaccag
    14161 ggccgttctt ataaatctta ccgtggtatg ggttccctgg gcgcgatgtc caaaggttcc
    14221 tctgaccgtt atttccagag cgataacgct gccgacaaac tggtgccgga aggtatcgaa
    14281 ggtcgcgtag cttataaagg tcgcctgaaa gagatcattc accagcagat gggcggcctg
    14341 cgctcctgta tgggtctgac cggctgtggt actatcgacg aactgcgtac taaagcggag
    14401 tttgtacgta tcagcggtgc gggcattcag gaaagccacg ttcacgacgt gaccattact
    14461 aaagagtccc cgaactaccg tctgggctcc tgattctctt cgcccgactt catgtcgggc
    14521 gatttatatt atctgtttca cttgcctcgg aataagcgtc aatgacggaa aacattcata
    14581 agcatcgcat cctcattctg gacttcggtt ctcagtacac tcaactggtt gcgcgccgcg
    14641 tgcgtgagct gggtgtttac tgcgaactgt gggcgtggga tgtgacagaa gcacaaattc
    14701 gtgacttcaa tccaagcggc attattcttt ccggcggccc ggaaagtact actgaagaaa
    14761 acagtccgcg tgcgccgcag tatgtctttg aagcaggcgt accgatattc ggcgtttgct
    14821 atggcatgca gaccatggca atgcagttgg gcggtcacgt tgaagcctct aacgaacgtg
    14881 aatttggcta cgcgcaggta gaagtcgtaa acgacagcgc actggttcgc ggtatcgaag
    14941 atgcgctgac cgcagacggt aaaccgctgc tcgatgtctg gatgagccac ggcgataaag
    15001 ttaccgctat cccgtccgac ttcgtcaccg tagccagcac cgaaagctgc ccgtttgcca
    15061 ttatggctaa cgaagaaaaa cgcttctatg gcgtacagtt ccacccggaa gtgactcaca
    15121 cccgccaggg tatgcgcatg ctggagcgtt ttgtgcgtga tatctgccag tgtgaagccc
    15181 tgtggacgcc agcgaaaatt atcgacgatg ctgtagctcg catccgcgag caggtaggcg
    15241 acgataaagt catcctcggc ctctctggtg gtgtggattc ctccgtaacc gcaatgctgc
    15301 tgcaccgtgc tatcggtaaa aacctgactt gcgtattcgt cgacaacggc ctgctgcgtc
    15361 tcaacgaagc agagcaggtt ctggatatgt ttggcgatca ctttggtctt aacattgttc
    15421 acgtaccggc agaagatcgc ttcctgtcag cgctggctgg cgaaaacgat ccggaagcaa
    15481 aacgtaaaat catcgggcgc gttttcgttg aagtattcga tgaagaagcg cagaaactgg
    15541 aagacgtgaa gtggctggcg cagggcacca tctaccctga cgttatcgaa tctgcggctt
    15601 ctgcaaccgg taaagcacac gtcatcaaat ctcaccacaa cgtgggcggc ctgccgaaag
    15661 agatgaagat gggcctggtt gaaccgctga aagagctgtt caaagacgaa gtgcgtaaga
    15721 ttggtctgga gctgggcctg ccgtacgaca tgctgtaccg tcatccgttc ccgggaccag
    15781 gccttggcgt tcgtgttctg ggtgaagtga agaaagagta ctgtgacctg ctgcgccgtg
    15841 ctgacgccat cttcattgaa gaactgcgta aaacggacct gtacgacaaa gtcagccagg
    15901 cgttcaccgt cttcctgcca gtacgttccg ttggcgtaat gggcgatggt cgtaagtatg
    15961 actgggttgt ctctctgcgt gctgtcgaaa ccatcgactt tatgaccgca cactgggcgc
    16021 atctgccgta cgatttcctc ggtcgcgttt ccaaccgcat tatcaatgaa gtgaacggta
    16081 tttcccgcgt ggtgtatgac atcagcggca agccgccagc taccattgag tgggaatgat
    16141 tttgatccct gcactatgaa tgaacaaaac cctccgttac tacagagggt tttttatctt
    16201 caagaatcat aggcttgaag ttactaacat cgattaatta aaccagctgt ccgatttgtt
    16261 ctcttctgct ttgcccacgc ttttcatcag atcgcgaccg ccttcagtca tatttctgtt
    16321 tgcgtcagct tcagattgca ccacatcggt ttgcgcagct ttgtgcttca gttcctgatc
    16381 gataaattcg ttttcgcgct taacgcgggc ttcttctttc gccagcgcca gtttttgttt
    16441 ctgaatctct aagctgcgta gctcatcttc ataactttga tcgcgttttt tgtccgcagt
    16501 ggcttcggcg tccagtttat cctgacgagc tttcttattt gccgctgccg ttgccgctct
    16561 tttgttcgcg gcggcctggg catttgcgcg acgttgcttc tcttgctgga tttccctgtt
    16621 gcgctccgcg acccattcgt catgctgcct ttgctcttca tttttacctt gctgttccgc
    16681 ttctgcgaca gcagagagtt gatcctgcaa tgatgaggct atagccggat agcttaagga
    16741 ggctaagatg gcgcaaagaa aaactttctt catgactcct cctgattatt agctcttttc
    16801 aggacatttg gtatttggct gaatacgcgt ttcgttatac gttgtggtaa taacaacggc
    16861 taaacctgtc gtaaactggc actctttacc ctcctgggtg gaggtataca ctttggtgcc
    16921 ttccttatat gttaaagaaa caccttccac taaggtttta tcattcacca tagaacccgc
    16981 tgccgcgcct acagctccgc cgccaactgc ccctgccgtc gttccggaat tgctgccaga
    17041 accgacgttg tggccgataa caccgccagc gactgcgcca ataagcgcgc cgaaggcttg
    17101 tgcgttccgt ttattttggg agttgtctac ggcaactttt gcgggaagaa tggaaataat
    17161 attaacggtt ttagtttctt gtttggtatt cagttgatcg gtttgataaa catcggcggc
    17221 atgatcgtca gcatttgact ggcatcctgc cagagtgaat gacgctaaca ttgccacagg
    17281 cagaagacat tttttaaatt tcatcactat tccttgttat acacattacg aaaaataaga
    17341 catttaagat catcaaaaga ggaaagtaaa tgtattgcgt ataaagggat agattaaatt
    17401 tattaaaatt cacgcaggga ataatttata aaaatatatc attacaacat atatattaat
    17461 tttatattta tttcgcagcc aaacaggata aacatcagtc aggacaaaga cgtaaaattt
    17521 aagatgaaag aatatctgcc gctttgataa tcccaaagag caaaagcatc cgacagcgaa
    17581 gtgccgttat gttctgtgaa agtggagaag gtaaccgcta acgttgtacc ggtgattcga
    17641 cggagagtgt ggattgctga gcttcttttt tgctctgatg gtgcagccag gcaccgacgg
    17701 acgagtagat aaaacggcca aagaagaaga taaagctgat gagcagtacg atacgggtca
    17761 tgcgactgtt aaatcggtgt cgttttcgca tactagttgc ctgactcaca aaaggttcct
    17821 tgaagtatgt cccacgctgt ggacggtact tacattaagg cacaacagga caaaatggtc
    17881 aattcttcgt tatgtaaaaa agcgtcagtg gatacatatt ttaatgttat ggaagttaat
    17941 ttaattattt acaatgatga tgtaataatg atgaattaac atgataaacg taataattca
    18001 ttaatcaggg ttattttgtt tgatatatat caattggaac tttcatatga cacttagaat
    18061 caatgctctc tatagtgatg aataatcatc attcgaagtc aggtgggatg cctgtctgaa
    18121 tacacctcct tcaggatgtg ggggattctg ctgagcatct atgaaactga atg
//
LOCUS       DADMEQ010000074        17649 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.074, whole genome shotgun sequence.
ACCESSION   DADMEQ010000074 DADMEQ010000000
VERSION     DADMEQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.074"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            144..473
                     /locus_tag="JNZ05_002577"
     CDS             144..473
                     /locus_tag="JNZ05_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY8565765.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            721..966
                     /gene="nrdH"
                     /locus_tag="JNZ05_002578"
     CDS             721..966
                     /gene="nrdH"
                     /locus_tag="JNZ05_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAY8565766.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            963..1373
                     /gene="nrdI"
                     /locus_tag="JNZ05_002579"
     CDS             963..1373
                     /gene="nrdI"
                     /locus_tag="JNZ05_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAY8565767.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            1346..3490
                     /gene="nrdE"
                     /locus_tag="JNZ05_002580"
     CDS             1346..3490
                     /gene="nrdE"
                     /locus_tag="JNZ05_002580"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAY8565768.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            3500..4459
                     /gene="nrdF"
                     /locus_tag="JNZ05_002581"
     CDS             3500..4459
                     /gene="nrdF"
                     /locus_tag="JNZ05_002581"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAY8565769.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            4814..6016
                     /locus_tag="JNZ05_002582"
     CDS             4814..6016
                     /locus_tag="JNZ05_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HAY8565770.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            6009..7073
                     /gene="proW"
                     /locus_tag="JNZ05_002583"
     CDS             6009..7073
                     /gene="proW"
                     /locus_tag="JNZ05_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HAY8565771.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGACSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            7131..8123
                     /gene="proX"
                     /locus_tag="JNZ05_002584"
     CDS             7131..8123
                     /gene="proX"
                     /locus_tag="JNZ05_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HAY8565772.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            8315..9494
                     /locus_tag="JNZ05_002585"
                     /pseudo
     CDS             8315..9494
                     /locus_tag="JNZ05_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            9618..10355
                     /locus_tag="JNZ05_002586"
     CDS             9618..10355
                     /locus_tag="JNZ05_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAY8565773.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            10345..10680
                     /gene="ygaH"
                     /locus_tag="JNZ05_002587"
     CDS             10345..10680
                     /gene="ygaH"
                     /locus_tag="JNZ05_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HAY8565774.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            10771..11301
                     /gene="emrR"
                     /locus_tag="JNZ05_002588"
     CDS             10771..11301
                     /gene="emrR"
                     /locus_tag="JNZ05_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HAY8565775.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPNQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            11428..12600
                     /gene="emrA"
                     /locus_tag="JNZ05_002589"
                     /pseudo
     CDS             11428..12600
                     /gene="emrA"
                     /locus_tag="JNZ05_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            12617..14155
                     /gene="emrB"
                     /locus_tag="JNZ05_002590"
     CDS             12617..14155
                     /gene="emrB"
                     /locus_tag="JNZ05_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HAY8565776.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(14219..14734)
                     /gene="luxS"
                     /locus_tag="JNZ05_002591"
     CDS             complement(14219..14734)
                     /gene="luxS"
                     /locus_tag="JNZ05_002591"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAY8565777.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGSHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(14884..16440)
                     /gene="gshA"
                     /locus_tag="JNZ05_002592"
     CDS             complement(14884..16440)
                     /gene="gshA"
                     /locus_tag="JNZ05_002592"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY8565778.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFHDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(16513..16941)
                     /locus_tag="JNZ05_002593"
     CDS             complement(16513..16941)
                     /locus_tag="JNZ05_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8565779.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(16938..17504)
                     /gene="yqaB"
                     /locus_tag="JNZ05_002594"
     CDS             complement(16938..17504)
                     /gene="yqaB"
                     /locus_tag="JNZ05_002594"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HAY8565780.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4180 a         4593 c         4497 g         4379 t
ORIGIN      
        1 ctcgttattt tcaaccgcgg gcctacctta cctgattgcg cattcacaac gctttcagaa
       61 aagtccataa agccgcgatg aacctcagtt aagaaaatta tggtctacac tgaaaattac
      121 atcgaattct aatgggggat catatgttta accgaccgaa ccgcaacgac gttgatgatg
      181 gcgtgcagga tatccagaat gatgtcaatc aattagctga cagcctcgaa tctgtattga
      241 agtcctgggg cagcgacgcc aaaggggaag ctgaagccgc acgcagtaaa gctcaggcgt
      301 tgctgaaaga aaccagggcg cgaatgcatg gtcgtactcg cgtccagcaa gccgcgcgcg
      361 atgccgttgg ctgcgcggat tcttttgttc gtgaaagacc ctggtgtagc gtgggtacag
      421 cagctgcggt aggtattttt atcggcgcac tgttaagcat gcgcaaatcg tagtgcaaaa
      481 atgataataa atacgcgtct ttgaccccga agcctgtctt cggggtttct ttttgcctgg
      541 tgaatcacaa aaatccccct cccccgtcac gctcatatcc agggtaattt cgatcactat
      601 ttgctatata ttgtgtggtt gaatcttttt tcaactacat ctagtatctc tgtatcaaca
      661 gagagacaac ccgacgcgta tcatcgcgcc gtatcttcat tttaaacaga aatacgaatc
      721 atgcgcataa ctatttatac tcgtaacgat tgcgttcagt gccacgccac caaacgggcg
      781 atggaaaacc ggggctttga ttttgaaatg attaatgtcg atcgcgttcc tgaagcagcg
      841 gaagcgttgc gtgctcaggg ctttcgtcag ttgccggtag tgattgctgg cgatcttagc
      901 tggtctggtt tccgtccgga catgattaac cgtctgcatc cagcgccaca cgcggccagt
      961 gcatgagcca gctcgtctac ttctccagca gctccgaaaa cacgcagcgt tttatcgaac
     1021 gtttaggtct gcccgcggtg cgcatcccgc tcaatgagcg ggaacggatt caggtagacg
     1081 agccttacat cctgatcgtg ccctcttacg gcggcggcgg tacggctggc gcggtgccac
     1141 gacaggtaat tcgcttttta aacgacgagc acaaccgggc gttgcttcgc ggcgttattg
     1201 cttctggtaa tcgcaacttt ggtgaggcgt atggccgcgc cggagatgtg attgcccgga
     1261 aatgcggcgt gccgtggctg taccgttttg aactcatggg tacgcaaagc gatatcgaaa
     1321 acgttcgtaa aggagtaacc gaattttggc aacgacaacc gcagaatgcc tgacgcagga
     1381 aacgatggat taccacgcgc tgaatgcgat gcttaatctt tacgatagcg caggtcgcat
     1441 tcagttcgat aaagaccgcc aggccgttga cgcctttatt gcgacgcatg tgcgtccgaa
     1501 cagtgtgacc ttcagtagcc agcagcagcg cctgaactgg ctggtcaacg aaggttacta
     1561 tgatgaaagc gttcttaatc gctactctcg cgactttgtc attacgctgt ttgcccacgc
     1621 acacaccagc ggttttcgtt tccagacatt cctcggggca tggaagtttt acaccagcta
     1681 tacgttgaag acattcgacg gtaaacgtta tctggaagat tttgccgatc gagtcacgat
     1741 ggtggcgctg acgctggcac aaggcgatga gacgctggcg ttgcaactga ccgatgaaat
     1801 gctgtcagga cgctttcagc cagccacgcc aacattcctc aactgcggta agcagcagcg
     1861 cggcgaactg gtttcctgtt ttttgctgcg tattgaagac aatatggagt cgattggtcg
     1921 ggcggtaaat tccgcactgc agctgtcgaa acgcggcggc ggcgtagcat ttttgctgtc
     1981 gaatctgcgg gaagcgggcg cgccaattaa acgtattgaa aatcaatctt ctggcgtaat
     2041 tccggtgatg aaaatgttgg aagacgcatt ttcctatgcc aaccaactcg gcgcgcgtca
     2101 gggggcaggt gcagtctatt tacatgctca tcatcccgat attctgcgtt ttctcgacac
     2161 gaaacgggaa aatgccgacg aaaaaatccg cattaaaaca ctgtcgctgg gcgtggtgat
     2221 cccggatatc actttccatc tggcaaaaga gaatgcgcag atggcgctgt tttcgcccta
     2281 tgacgttgag cgagtttatg gcaagccgtt tgccgatatc gccattagcg aacactatga
     2341 cgaactggtt gccgatgaac gcatccgcaa aaaatacctc aacgcccgtg atttcttcca
     2401 gcgactggca gaaatccagt ttgagtccgg ctatccctac atcatgtatg aagacacagt
     2461 aaaccgtgct aaccctatcg ccgggcgcat aaatatgagt aatctctgct cagaaatttt
     2521 gcaggttaac agcgcctcag agtatgacga gaatctcgac tatgcccgca caggccatga
     2581 tatttcctgc aatttaggtt cgttgaatat tgcgcacacc atggattccc ccgatttttc
     2641 ccgcacggta gagactgccg tgcgcggttt aacggcggtg tcagatatga gtcatatccg
     2701 cagcgtgccg tccatcgaag ccggaaatgc cgcctcgcac gccatcggac tggggcagat
     2761 gaatttacat ggttatctgg cgagggaagg catcgcttat ggttcgccgg aagccctgga
     2821 tttcaccaat ctctatttct ataccatcac ctggcacgca ctgcgtacct cgatgttgct
     2881 ggcgcgcgaa cgcggtgaaa ccttcgccgg gttcaaacag tcacgctatg ccagtggtga
     2941 atatttcagc caatatctgc aaggtaactg gcagccgaaa acggcgaaag ttggcgaact
     3001 gtttgcccgc agcggtatta cgttacctac ccgtgagatg tgggcgcaac tgcgcgacga
     3061 cgtgatgcgc tacggcatat acaaccagaa tctccaggcg gtgccgccaa ccgggtctat
     3121 ctcttatatc aaccatgcta cgtcgagtat tcatccgatt gtggcgaaag tagagatacg
     3181 caaagagggc aaaacaggac gcgtttacta ccctgccccg tttatgacta acgaaaatct
     3241 ggcgctgtat caggacgctt acgaaattgg cgcagaaaag atcatcgaca cctacgcgga
     3301 agcgactcgc catgtcgatc aggggctgtc gctgacgctg tttttccccg ataccgccac
     3361 cactcgcgat atcaacaaag cgcagattta cgcctggcgc aaaggtatca aaacgctcta
     3421 ttacatccgc ctgcgtcaga tggcgctgga aggcactgaa attgaaggct gcgtctcctg
     3481 tgcactttaa ggaatatcta tgaaactctc acgtatcagc gccatcaact ggaacaagat
     3541 atctgacgat aaagatctgg aggtgtggaa tcgcctgacc agcaatttct ggctaccaga
     3601 aaaggtgccg ctgtcgaacg atattcccgc ctggcagaca ttaactgtcg tagaacaaca
     3661 actgacgatg cgcgttttta ctggcctgac gctgctcgac acgctgcaaa atgttatcgg
     3721 cgcgccttcg ctgatgcccg atgcgctcac gcctcatgaa gaggcggtat tatcgaatat
     3781 cagctttatg gaagcggttc atgcccgctc ttacagttcg attttctcga cgctgtgcca
     3841 gaccaaagat gtcgatgccg cctacgcctg gagtgaagaa aacgcaccgt tgcagcgaaa
     3901 agctcagatt attcagcaac attatcgcgg tgatgatccg ctgaaaaaga aaatcgccag
     3961 tgtgtttctt gaatcttttt tgttctattc cggtttctgg ctgccgatgt atttttccag
     4021 ccgcggaaag ctgaccaata ccgcggacct gatccgtctg attatccgcg atgaagcagt
     4081 ccacggttac tacataggct ataaatatca gaaaaacatg gaaaagatat ctctgggaca
     4141 acgtgaagag ttgaagagtt tcgccttcga tttgttgctg gaactctacg acaacgagtt
     4201 gcaatacacc gatgagctgt acgccgaaac cccgtgggct gacgatgtga aagcgtttct
     4261 ctgttacaac accaataagg ctttgatgaa tctgggctac gaaccgttat ttcccgcaga
     4321 aatggcggaa gtgaatccgg caatcctcgc cgcgctttcg ccgaatgccg atgaaaatca
     4381 cgatttcttt tccggttcag gctcctctta tgtgatgggg aaagcggttg aaacagaaga
     4441 tgaagactgg aatttctgag ggtgttattt tcaaaaatat cactacccgc agcagggaaa
     4501 taattcccgc caaatagctt tttatcacgc aaatattctg tagtgatcta cactgatact
     4561 ctgttgcatt attcgcctga aactacaata ttcaggcgtt ttttcgctat ctatgacaaa
     4621 aaatatcaac ttttctcgat ttgctctcag cccttatatc acggaaaatt ccggcgattt
     4681 gctcgcatca atattcatgc cacatttgcc ctcaggggtt gcctcagatt ctcagtatgt
     4741 tagggtagaa aaaagtgact attcccattg ggtaatatat cgacataaac aaataaagga
     4801 atcattctat tgcatggcca ttaaattaga aattaaaaat ctttataaaa tatttggtga
     4861 gcatccacag cgagcgttca aatatatcga acaaggactt tcaaaagaac aaattctgga
     4921 aaaaactggg ctatcgcttg gcgtaaaaga cgccagtctg gccattgaag aaggcgagat
     4981 atttgtcatc atgggattat ccggctcggg taaatccaca atggtacgcc ttctcaatcg
     5041 cctgattgaa cccacccgcg ggcaagtgct gattgatggt gtggatattg ccaaaatatc
     5101 cgacgccgaa ctccgtgagg tgcgcagaaa aaagattgcg atggtcttcc agtcctttgc
     5161 cttaatgccg catatgaccg tgctggacaa tactgcgttc ggtatggaat tggccggaat
     5221 taatgccgaa gaacgccggg aaaaagccct tgatgcactg cgtcaggtcg ggctggaaaa
     5281 ttatgcccac agctacccgg atgaactctc tggcgggatg cgtcaacgtg tgggattagc
     5341 ccgcgcgtta gcgattaatc cggatatatt attaatggac gaagccttct cggcgctcga
     5401 tccattaatt cgcaccgaga tgcaggatga gctggtaaaa ttacaggcga aacatcagcg
     5461 caccattgtc tttatttccc acgatcttga tgaagccatg cgtattggcg accgaattgc
     5521 cattatgcaa aatggtgaag tggtacaggt cggcacaccg gatgaaattc tcaataatcc
     5581 ggcgaatgat tatgtccgta ccttcttccg tggcgttgat attagtcagg tgttcagtgc
     5641 gaaagatatt gcccgccgga caccgaatgg cttaattcgt aaaactcctg gcttcggccc
     5701 acgttcggca ctgaaattat tgcaggatga agatcgcgaa tatggctacg ttatcgaacg
     5761 cggtaataag tttgtcggcg cagtctccat cgattcgctt aaaaccgcgt taacgcagca
     5821 gcaaggtctt gatgcggcgc tgattgatgc gccgttagca gtcgatgcac aaacgcctct
     5881 tagcgagttg ctctctcatg tcggacaggc accctgtgcg gtgcccgtgg tcgacgagga
     5941 ccaacagtat gtcggcatca tttcgaaagg aatgctgctg cgcgctttag atcgtgaggg
     6001 ggtaaataat ggctgatcaa aataatccgt gggataccac gccagcggcg gacagtgccg
     6061 cgcaatccgc agacgcctgg gggacaccga cgactgcacc ggctgacggc ggtggtgctg
     6121 actggctgac cagtacgcct gcgccaaacg tcgagcattt taatattctc gatccgttcc
     6181 ataaaacgct gatcccgctc gacagttggg tcactgaagg gatcgactgg gttgttaccc
     6241 atttccgtcc cgtcttccag ggcgtgcgcg ttccggttga ttatatcctc aacggtttcc
     6301 agcaattgct gctgggtatg ccagcgccag tggcgattat cgttttcgct ctcatcgcct
     6361 ggcagatttc cggggtcgga atgggcgtgg cgacgctggt ctcgctgatt gccatcggcg
     6421 caatcggtgc ctgttcgcag gcaatggtga ctctggcgct ggtgttaact gccctgctgt
     6481 tctgtatcgt catcggtttg ccgttgggga tatggctggc gagaagtccg cgagcggcga
     6541 aaattattcg tccactgctt gatgccatgc agaccacgcc agcgtttgtt tatctggtgc
     6601 caatcgtcat gctatttggt atcggtaacg tgccgggcgt ggtggtgacg atcatctttg
     6661 cgctgccgcc gattatccgt ctgaccattc tggggattaa ccaggttccg gcggatctga
     6721 ttgaagcctc gcgctcattc ggtgccagcc cgcgccagat gctgttcaaa gttcagttac
     6781 cgctggcgat gccgaccatt atggcgggcg ttaaccagac gctgatgctg gccctttcta
     6841 tggtggtcat tgcctcgatg attgccgtcg gcgggctggg tcagatggta cttcgcggta
     6901 tcggtcgtct ggatatgggg cttgccaccg ttggcggcgt cgggattgtg atcctcgcca
     6961 ttatcctcga tcgcctgacg caggccgttg ggcgcgactc acgcagtcgc ggcaaccgtc
     7021 gctggtacac cactggccct gttggtctgc tgacccgccc attcattaag taactctgca
     7081 cttgcccggt gacgccgggc attatcaccc tgccaaaaaa aggaataaca atgcgacata
     7141 gcgtactttt tgcgacagcg tttgccacgc ttatctctac acaaactttt gctgccgatc
     7201 tgccgggcaa aggcattact gttaatccag ttcagagcac catcactgaa gaaaccttcc
     7261 agacgctgct ggtcagtcgt gcgctggaga aattaggtta taccgtcaac aaacccagcg
     7321 aagtggatta caacgttggc tacacctcgc ttgcttccgg cgatgcaacc ttcaccgccg
     7381 tgaactggac gccactccat gacaacatgt acgaagctgc cggtggcgat aagaaatttt
     7441 atcgtgaagg ggtatttgtt aacggcgcag cacagggtta cctgatcgat aagaaaaccg
     7501 ccgaccaata caaaatcacc aacatcgcac aactgaaaga tccgaagatc gccaaactgt
     7561 tcgataccaa cggcgacggt aaagcggatt taaccggttg taaccctggc tggggctgcg
     7621 aaggtgcgat caaccaccag cttgccgcgt atgaactgac caacaccgtg acgcataatc
     7681 aggggaacta cgcagcgatg atggccgaca ccatcagtcg ctacaaagag ggcaaaccgg
     7741 tgttttatta cacctggacg ccgtactggg tgagtaatga gctgaagcca gggaaagatg
     7801 tggtctggtt gcaggtgccg ttctccgcac tgccgggcga taaaaatgcc gataccaaac
     7861 tgccgaatgg cgcgaattac ggcttcccgg tcagcaccat gcatatcgtt gccaacaaag
     7921 cctggaccga gaaaaatccg gcggcagcca aactgtttgc cattatgcag ttgccagtgg
     7981 ccgatattaa cgcccagaac gccattatgc atgacggcaa agcctcagaa ggcgatattc
     8041 agggccacgt tgatggctgg atcaaagccc accagcagca gttcgatggc tgggtgaatg
     8101 aggcgctggc agcgcagaag taatttttat tcgggcggat aaggcgtttt cgcctcatcc
     8161 gccgctctgt acacaatgcc tgatgcgacg ctgacgcgtc ctatcatgcc tacaaaccac
     8221 atcgcacatt tcaacaagct atctttcatc ccatattcat caacatccgc tattattgat
     8281 ttccagctta atcatcacct gatgaacaaa aataatgact aaacctaatc atgagcttag
     8341 cccggcgctg atcgtgctga tgtctatcgc caccggtctg gcggtagcca gtaactatta
     8401 cgcccagcca ttgctcgaca ccatcgcgcg taacttttcc ctttccgcca gttcggcagg
     8461 ctttattgtt accgccgcgc agttgggcta tgccgcaggt ctactgtttc ttgttcccct
     8521 cggtgatatg tttgaacgcc gccgcctgat tgtctcgatg accttactgg cagcgggcgg
     8581 tatgttaatc actgccagca gtcagtcact ggcgatgatg atcctcggta cggcattaac
     8641 cggtttattc tcagtcgtgg cacaaattct ggttccgctg gcagcgacgc tggcttcacc
     8701 ggataaacgc ggcaaagtgg ttggcaccat tatgagcggt ctgctgttgg ggatattgct
     8761 ggcacgaacc gttgccgggt tactggcaaa cctcggcggc tggcgcaccg tcttttgggt
     8821 tgcttcggtg ttaatggcac tgatggcgct ggcattatgg cgtggtctgc cacaaatgaa
     8881 atcagaaacc cacctcaact acccacagtt gttgggttcc gttttcagca tgttcatcag
     8941 cgataagatc ctgcgcaccc gcgcgttgct gggctgcatg acctttgcca acttcagcat
     9001 tctctggacc tcaatggcct ttttgcttgc cgctccacct tttaactaca gcgatggtga
     9061 cattggtctg tttgggcttg cgggagctgc cggagcgttg ggcgctcgtc cggcgggcgg
     9121 ttttgccgac aagggcaaat cgcaccacaa ctttcggtct gcttctgctg ttactttcat
     9181 ggctggcaat ctggtttggt cacacttccg tactggcgtt gattatcggc attctggtgc
     9241 tggacctcac cgtacagggc gtgcatatca ctaaccagac ggtaatttat cgaatacatc
     9301 ctgatgcgcg taatcgcctg accgcaggtt acatgaccag ctactttatt ggcggtgccg
     9361 ccggttcgct aatttcagcc tcagcctggc aacatggcgg ttgggctggc gtttgtctgg
     9421 ctggcgcgac gattgccctg gttaacttac tggtctggtg gcgaggtttt catcgtcagg
     9481 aagccgcaaa ttaagcaatc agcaaccgtt ttcgtggctt tacacttata agggtgttaa
     9541 gaagcccatc agtctgataa ggttaagata ttcattcagt ctatttataa tattaacaat
     9601 cgttaagcgt acactctatg gaaagcccta ctccacagcc tgctcctggt tcggcgacct
     9661 tcatggaagg atgcaaagac agtttaccga ttgttattag ttatattccg gtggcctttg
     9721 cgttcggtct gaatgcgacc cgtctgggat tctctcctct cgaaagcgtt tttttctcct
     9781 gcatcattta tgcaggcgcg agccagttcg tcattaccgc gatgctggca gccgggagta
     9841 gtttgtgggt tgctgcactg accgtcatgg caatggatgt tcgccatgtt ttgtatggcc
     9901 cgtcactgcg tagccgtatt attcagcgtc tgcaaaaatc gaaaaccgcc ctatgggcgt
     9961 ttggcctgac ggatgaggtt tttgccgccg ccaccgcaaa actggtacgc aataatcgcc
    10021 gctggagcga gaactggatg atcggcattg ccttcagttc atggtcatcg tgggtatttg
    10081 gtacggtaat aggggcattc tccggcagcg gcttgctgca aggttatccc gccgttgaag
    10141 ctgcattagg ttttatgctt ccggcactct ttatgagttt cctgctcgcc tctttccagc
    10201 gcaaacaatc tctttgcgtt accgcagctt tagttggtgc ccttgcaggc gtaacgctat
    10261 tttctattcc cgtcgccatt ctggcaggca ttgtctgtgg ctgcctcact gcgttaatcc
    10321 aggcattctg gcaaggagcg cccgatgagc tatgaggttc tgctgcttgg gttactggtt
    10381 ggcgcggcga attattgctt ccgctatttg ccgctgcgcc tgcgtgtggg taatgcccgc
    10441 ccaaccaaac gtggcgcggt aggtattttg ctcgacacca ttggcatcgc ctcgatatgc
    10501 gctctgctgg ttgtctctac cgcaccagaa gtgatgcacg atacacgccg tttcgtgccc
    10561 acgctggtcg gcttcgcggt actgggtgcc agtttctata aaacacgcag cattatcatc
    10621 ccaacactgc ttagtgcgct ggcctatggg ctcgcctgga aagtgatggc gattatataa
    10681 cccacaagaa tcatttttct aaaacaatac atttacttta tttgtcactg tcgttactat
    10741 atcgactgaa attaatgagg tcatacccaa atggatagtt cgtttacgcc cattgaacaa
    10801 atgctaaaat ttcgcgccag ccgccacgaa gattttccta atcaggagat ccttctgact
    10861 cgtctttgca tgcacatgca aagcaagctg ctggagaacc gcaataaaat gctgaaggct
    10921 caggggatta acgagacgtt gtttatggcg ttgattacgc tggagtctca ggaaaaccac
    10981 agtattcagc cttctgaatt aagttgtgct cttggatcat cccgtaccaa cgcgacgcgt
    11041 attgccgatg aactggaaaa acgcggttgg atcgaacgtc gtgaaagcga taacgatcgc
    11101 cgctgcctgc atctgcaatt aacggaaaaa ggtcacgagt ttttgcgcga ggttttacca
    11161 ccgcagcata actgcctgca tcaactctgg tccgcgctca gcacaacaga aaaagatcag
    11221 ctcgagcaaa tcacccgcaa attgctctcc cgtctcgacc agatggaaca agacggtgtg
    11281 gttctcgaag cgatgagcta acgcgtcatc tcgctcaaaa atccagattt ataaaagaaa
    11341 aaatgaccgg ccagcatcgc aacatgctgg cctttttggc aagcaggtcg gctcagccga
    11401 tgagttaaga agatcgtgga gaacaatatg agcgcaaatg cggagactca aaccccgcag
    11461 caaccggtaa agaagagcgg caaacgtaag cgtctgctcc tccttctcac cttgctcttt
    11521 ataattattg ccgtagcgat agggatttat tggtttttgg tactgcgtca tttcgaagaa
    11581 accgatgacg catacgtggc agggaatcag attcaaatta tgtctcaggt gtctggcagc
    11641 gtgacgaaag tctgggccga taacaccgat tttgtaaaag aaggcgatgt tctggtcact
    11701 ctcgacccga cagatgctcg ccaggcgttt gaaaaagcca aaactgcact ggcttccagc
    11761 gttcgccaaa cccaccagct gatgattaac agcaaacagt tgcaggcgaa tattgaggtg
    11821 caaaaaatcg ccctcgcgaa agcacaaagc gactacaacc gccgtgtgcc gctgggcaat
    11881 gccaacctga ttggtcgcga agagctgcaa cacgcccgcg acgccgtcac cagtgcccag
    11941 gcgcaactgg acgtcgctat tcaacaatac aatgccaatc aggcgatgat tctggggact
    12001 aaactggaag atcagcctgc cgtgcaacag gctgccaccg aagtacgtaa cgcctagctg
    12061 gcgctggagc gtacccgtat tgtcagtccg atgaccggtt atgtttcccg tcgtgccgtg
    12121 caaccgggtg cgcaaattag cccaacgacg ccgctgatgg cggtcgttcc agccaccaat
    12181 atgtgggtgg atgccaactt taaagagacg cagattgcca atatgcgcat cggtcagccg
    12241 gtcaccatca ccaccgatat ttacggcgat gatgtgaaat acaccggtaa agtggttggt
    12301 ctggatatgg gcacaggtag cgcgttctca ctgcttccgg cgcaaaatgc gaccggtaac
    12361 tggatcaaag tcgttcagcg tctacctgtg cgtatcgaac tggaccagaa acagctggag
    12421 caatacccgc tgcgcattgg tttgtccacg ctggtaagcg tcaataccac taaccgtgac
    12481 ggtcaggtac tggcaaataa agtacgttca acaccggtcg cggtaagtac cgcgcgtgaa
    12541 atcagcctag cacctgtcaa taaactgatc gacgatatcg taaaagctaa cgctggctaa
    12601 tccagaggtg cgtgtgatgc aacagcaaaa accgctggaa ggcgcgcaac tggtcattat
    12661 gacgattgcg ctgtcactgg cgacattcat gcaggtgctg gactccacca ttgctaacgt
    12721 ggcgatcccc actatcgccg ggaatctggg ctcatcgctc agccaaggaa cgtgggtaat
    12781 cacttctttc ggggtggcga atgccatctc gatcccgctt accggctggc tggcaaagcg
    12841 cgtcggggaa gtgaaactgt tcctttggtc caccatcgcc tttgctattg cgtcgtgggc
    12901 gtgtggtgtc tccagcagcc tgaatatgct gatcttcttc cgcgtgattc aggggattgt
    12961 cgccgggccg ttgatcccgc tttcgcaaag tctattgctg aataactacc cgccagccaa
    13021 acgctcgatc gcgctggcgt tgtggtcgat gacggtgatt gtcgcgccaa tttgcggccc
    13081 gatcctcggc ggttatatca gcgataatta ccactggggc tggatattct tcatcaacgt
    13141 gccgattggc gtggcggtgg tgttgatgac actgcaaact ctgcgcggac gtgaaacccg
    13201 caccgaacgg cggcggattg atgccgtggg gctggcactg ctggttattg gtatcggcag
    13261 cctgcagatt atgctcgacc gcggtaaaga gctggactgt ttttcatcac aggaaattat
    13321 cattcttacc gtggtggcgg tggtggctat ctgcttcctg attgtctggg agctgaccga
    13381 cgataacccg atagtcgatc tgtcgttgtt taagtcgcgc aacttcacca tcggctgctt
    13441 gtgtatcagc ctcgcgtata tgctctactt cggcgctatt gttctgctgc cgcagttgtt
    13501 gcaggaggtc tacggttaca cggcgacctg ggcaggtttg gcctctgcgc cggtagggat
    13561 tattccggtg attctgtcgc cgattatcgg ccgcttcgcg cataaactgg atatgcggcg
    13621 gctggtaacc ttcagcttta ttatgtatgc cgtctgcttc tactggcgtg cctatacctt
    13681 tgaaccaggt atggattttg gcgcgtcggc ctggccgcag tttatccagg ggtttgcggt
    13741 ggcctgcttc tttatgccgc tgaccaccat tacgctgtct ggtttgccac cggaacgact
    13801 ggcggcggca tcgagcctct ctaactttac gcgaacgctg gcggggtcta tcggcacgtc
    13861 gataaccacg accatgtgga ccaaccgcga gtcgatgcac catgcgcagt tgactgagtc
    13921 ggtaaacccg ttcaacccga atgcccaggc gatgtacagt caactggaag ggcttgggat
    13981 gacgcaacag caggcatcag gctggattgc ccagcagatc accaatcagg ggctgattat
    14041 ttccgccaat gagatcttct ggatgtcagc cgggatattc ctcgtactgc tggggctggt
    14101 gtggtttgct aaaccgccat ttggcgcagg tggcggcgga ggcggtgcgc actaagtaca
    14161 actaagccag ttcatttgaa ctggcttttt tcaattaatt gtgaagatag tttactgact
    14221 agatgtgcag ttcctgcaac ttctctttcg gcagtgccag ttcttcgttg ctgttgatgc
    14281 gtacgtcacg ttccagaatg ctacgcgcaa tatcctgcgc ttcctgcaac gagtgcatct
    14341 ggtaagtgcc acactggtag acgttcagtt ccgggatctg attctgatcc tgcactttca
    14401 gcacgtcttc cattgccgct ttccaggcat cagcaacacg ctgctcatct ggcgtaccaa
    14461 tcagactcat ataaaaaccg gtgcggcagc ccattggcga gatatcgata atctctacac
    14521 cattaccgtt aagatggtta cgcataaaac cagcaaacag gtgctccagg gtatggctcc
    14581 ctctttctgg catcacttct ttgttcggca cgcagaagcg cagatcgaac acggtgattg
    14641 cgtcgccatg cggggtgttc attgttttcg ccacccgaac tgcaggcgct tccatccggg
    14701 tatgatcgac tgtgaagcta tctaacaacg gcatttagcc acctccggta atttttttaa
    14761 aaattttctg aactctttct tcccaggcga gtctgagtat atgaaagacg cgcatttgtt
    14821 atcatcatcc ctgaattcag agatgaaatt ttggccactc acgagtggcc tttttctttt
    14881 ctgtcaggcg tgtttttcca gccacaccgc aaacggttcg gtatcagcgg tttccatttc
    14941 ctgctgacgg cgttcagatg cctcgcgctc ggctacaaaa tcctcttcgc gcagaatttc
    15001 cagcggctct tcacgcagca gattacggta ggcttctgca aacgctttgc ctgttccgcc
    15061 aataccagta tcaatcatag accttaagat acgggcagag aaagtcagat cgggattatc
    15121 gaagcaggca accagttcat cacacacttt ctgatacgct tcgccgccgt taatgctatc
    15181 cagcgtttgc gcgacgcgtt tcagatcgtg gaacagatct ttacccacct gcagtaacgg
    15241 gaactgcgcg gtttcgcagc cgatacccag cgtcaggccc ggtttgcgac cttcaaggat
    15301 cacccggttc cagttaacgc gcgtacaggc aagttcgcta ctgctcattt ccggtgcatc
    15361 agccagcgca caccagacca taaacaggtc gaggaatcgc acctgctgtt catctacacc
    15421 aatcggcgag aacgggttga tgtccagcga acgcacttca atatattcaa tgccgccacg
    15481 taacagcgca tcagaaggtg actcgccgct gcgggtaacg cgttttggac gaatcggcgc
    15541 atacagttcg ttttcaatct gcagcacgtt gctattgatt tgcagcctct taccgtcttt
    15601 ctcaatacca atcttcgcgt actcttccga tggcgttttg attgcctgtt taaggcccgc
    15661 tacgtactcg taaagatcgt tgaaggtaat accaagattg ctttgcgatt tattggtata
    15721 gccgagatcg ctcaaacgaa gagaggtcgc atacggcagg taatacatac cgcactcggt
    15781 tttctcaaac ggcagcgacg ttggttttcc ttgcaggaaa gaagaacaaa tcgccggaga
    15841 tgcaccaaac agataaggaa tgacccaacc gaaacgatag taattgcgga taacgcggaa
    15901 atagcccgca gaaattttct ctttggcatc agcgcccgag atatcaccgc acttcgcttg
    15961 ccagaatgcc attggcaaag agaaattgta gtgcacgccg gaaatggttt gcatcagcgc
    16021 gccgtagcga tttttcagcc cttcacgata cagcgtttta aagcgtccgg tgttagaagt
    16081 gccgtactgt gccagttcga tgtcctgacc ttctgcgatg tagcatggca tacttaacgg
    16141 ccacatccgc tcatcgccca tattgcgcgc cgtataacga tgcagatcgc gcataaaggt
    16201 cagcatatgt tcaatatcac catccactgg tgtaatgaat tccagcaatg cttccgcaaa
    16261 atcggtagta atccatttgt gcgtcagtgc ggaacctaat gcttcaggat gacctgttgt
    16321 tgccagtgtg ccatcagcat taacacgcaa agtttcgcgc tccagcccac gctgtatccc
    16381 ctttaacgcc tgaggatgtt tttccagcca ggccagcgcc tgtgatacgt ccgggatcaa
    16441 attgacctcc cgcctgtcaa aatcgtttta attagcataa ctgtaatggt gaccatatgt
    16501 gcaggcctac aattagtgcc accacatcat gccctgaacg gtcgctgctg caactgcaac
    16561 atagcgtaac gctttaccaa ggcacaaaaa aaagattact ggcccccagg agatgcgcat
    16621 ccatcccgct aacagacaca gtaaatcgcc aaccacgggc atccagctta ataatagcgt
    16681 gactgcacca tagcgtttca gccagccggt agctttctct tgccagcgcg atgttttacg
    16741 caatggaaag aaacgcccaa ggataacgtt agttaaccct ccaaggctat tacccattgt
    16801 tgctgttaaa actaaaaccc agggatgact gatcccggaa agcaacattg ccaccagcac
    16861 gacttcggag ttgccgggta atagtgtagc gctgagaaaa ctactggcaa acaacgagaa
    16921 aagcgataac gcttcactca cagcaagcga acatccacag cgtccatgcc tgctgcacgg
    16981 gccgcctgaa taccgaaatc ggcatcttca aagaccacac actgcgtcgg ttgcacgccc
    17041 atacgctgcg cgcacaacaa aaatgtgtct ggcgcgggtt tatggtgttt gacgtgatca
    17101 gcagcgacga cggcgtcaaa ataacggcgt aatcccaggt gcgccagcaa tgcctctgcg
    17161 atggcgcttt cactccccgt tcctacagcc attgggcgac gaccatgcca acttttcacc
    17221 acttcaacaa gaggaagcgg ctcgacacta tccagcagca tacttcttac tgcttctgtt
    17281 ttttcacgcg ctaacgcatg cgggtcgaga tcggcctgat tcagctcaat aattgcctga
    17341 gcaatacgcc aggtgggcga tccattaagc gcaatcatcg cctgaatatc gtactgaaga
    17401 ccgtagtgcc ctaatacttc gcgccacgct ttacggtgcg taggctccgt atccaggatt
    17461 gtgccatcca tatcaaaaat taaacctgca taacgctcgt acatggtctt ctcgcaagtc
    17521 gaaaaatatg acgctacttt agcgtaattg cttgattttg tcgctgatgg cgaaggagaa
    17581 gaaaattcag gaaatggata aagtagttat ttagaaatga gatatttttg aaggaaattt
    17641 tttggagaa
//
LOCUS       DADMEQ010000075        17458 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.075, whole genome shotgun sequence.
ACCESSION   DADMEQ010000075 DADMEQ010000000
VERSION     DADMEQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17458
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.075"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            564..1334
                     /locus_tag="JNZ05_002595"
     CDS             564..1334
                     /locus_tag="JNZ05_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HAY8565781.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="JNZ05_002596"
     CDS             1325..1798
                     /locus_tag="JNZ05_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAY8565782.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGTLSIGLSIPGLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="JNZ05_002597"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="JNZ05_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HAY8565783.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="JNZ05_002598"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="JNZ05_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HAY8565784.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGVIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="JNZ05_002599"
     CDS             3233..3727
                     /locus_tag="JNZ05_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HAY8565785.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="JNZ05_002600"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="JNZ05_002600"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HAY8565786.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /locus_tag="JNZ05_002601"
     CDS             complement(6410..7432)
                     /locus_tag="JNZ05_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAY8565787.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFVISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7571..8484
                     /locus_tag="JNZ05_002602"
                     /pseudo
     CDS             7571..8484
                     /locus_tag="JNZ05_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            8699..10060
                     /locus_tag="JNZ05_002603"
     CDS             8699..10060
                     /locus_tag="JNZ05_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8565788.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="JNZ05_002604"
     CDS             complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="JNZ05_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY8565789.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="JNZ05_002605"
     CDS             complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="JNZ05_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HAY8565790.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12610..13899
                     /locus_tag="JNZ05_002606"
     CDS             12610..13899
                     /locus_tag="JNZ05_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HAY8565791.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13896..15362
                     /gene="hpaE"
                     /locus_tag="JNZ05_002607"
     CDS             13896..15362
                     /gene="hpaE"
                     /locus_tag="JNZ05_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HAY8565792.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLTDVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTANRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATERNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKLSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15364..16215
                     /gene="hpaD"
                     /locus_tag="JNZ05_002608"
     CDS             15364..16215
                     /gene="hpaD"
                     /locus_tag="JNZ05_002608"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HAY8565793.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16225..16605
                     /locus_tag="JNZ05_002609"
     CDS             16225..16605
                     /locus_tag="JNZ05_002609"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HAY8565794.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16673..>17458
                     /gene="hpaH"
                     /locus_tag="JNZ05_002610"
     CDS             16673..>17458
                     /gene="hpaH"
                     /locus_tag="JNZ05_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HAY8565795.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSI"
BASE COUNT         4296 a         4626 c         4374 g         4162 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgttt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tggggtctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtacgtt
     1741 atccatcggt ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggtg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctaggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaggcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact aaatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgcccg ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccagcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc tgacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaataacga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cccaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tctccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcgt
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacggttt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg cttcagctgc cgacaatatc ttttttgatc aatcgcttga gatcatctca
     8281 gtcatctttc attttcacta tcaatgggac gaaagcgatc ttaaacagcg caatatcatt
     8341 gccgtcgatg aacatatgac cattctcagc gctctgattt gccgcagtga ccttgatgcc
     8401 acccttgcgc tccgtaacca tttgaattca gctaaacaat caatgattcg atccattaat
     8461 gagaacacgc gttatgctca ttaagtacgg attaaaaaca gcaaactatt agcgggtgca
     8521 cggttttatc aaaagataaa taccgggaaa ttcccgctta cctatgctca caatcagtcg
     8581 actaaaaatg tttttgtgag cggtaataaa gtcctaaaac tttaacctgc cgttaatgtc
     8641 aaccttgcgt aatgcacaat tacgtaaaca attaaaatta taaatacagg agtccggcgt
     8701 ggaaaaagaa aatatcaccc tcgatccgcg ttcttcattt actccatctt cgtcggcaga
     8761 tattcccgtg ccaccagatg gattagttca acgcagtacc cgaattaaac gcattcaaac
     8821 caccgccatg ttgttattat tttttgcggc ggtaatcaat tatctcgacc gcagttcgct
     8881 gtcggtagca aatttaacga ttcgtgaaga attgggatta agtgccaccg aaatcggcgc
     8941 tttgctctcc gtgttttcac tcgcttacgg cattgcgcaa cttccttgcg gcccactatt
     9001 ggatcgtaaa ggcccacgcc tgatgctggg actggggatg ttcttctggt cactgttcca
     9061 ggccatgtct ggcatggtgc acagctttac gcagttcgtg ttggtgcgta tcggtatggg
     9121 gattggtgaa gcgccgatga acccatgcgg tgtaaaagtc attaacgact ggttcaacat
     9181 caaagagcgc ggacgcccga tgggcttctt caacgcagct tctaccattg gcgttgcggt
     9241 aagcccaccg attctggcgg caatgatgct ggtgatgggc tggcgcggga tgtttattac
     9301 cattggtgta ctggggattt ttctcgccat cggctggtat atgctctatc gcaaccgcga
     9361 gcacgtagaa ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg tcaatgcccg
     9421 ccgagatccg ctcaattttg ccgaatggcg cagcctgttc cgtaaccgca caatgtgggg
     9481 aatgatgctc ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg cctggcttcc
     9541 tggttacctg caaacagcct ataacctgga tttaaaaagc acagggttga tggcggctat
     9601 ccctttcctg tttggggctg ccgggatgct ggtcaacggt tacgttactg actggctggt
     9661 caaaggggga atggctccga ttaaaagccg taagatctgc attattaccg ggatgttctg
     9721 ttctgccgcc tttacgctga tagtaccaca agcgacaaca tccatgatgg cggttctgct
     9781 gattggcatg gcactgttct gtattcactt tgccggaaca tcctgctggg gcttgatcca
     9841 cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt atccagaact ttgccagctt
     9901 catctgcgcc tctttcgcgc cgatcattac tggttttatt gttgatacaa cccactcatt
     9961 ccgtctggca ctaatcattt gcggttgcgt caccgcagcg ggagcactgg cgtacatctt
    10021 cctggttcgc cagccgatca acgacccgcg taaagattaa actccctatg cccgataaca
    10081 ttttgcttat cgggcatttt tatggcgatt aaaacgtttc ccagttctcc ccgctatctg
    10141 ccactgccat tttacgcggc gtagctggcg tcacggtttt taccacagcc gatgtttcac
    10201 gctgctgttg ctgctgttgc tgaatccgga acactgccac agcttcggtc aggcgactgg
    10261 cctgctcttc cagcgcggcg gcggcagcgg cagattcttc caccagcgcg gcgttctgtt
    10321 gagttacccg gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct
    10381 catcagaagc agaagctatt tcgcccataa tgtccgtcac gcgggtcaca gcactgacaa
    10441 tctccgccat tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca
    10501 ccgagtcttc aatcaggctt ttaatttcac gagccgcctg ggcgctacgc tgggccagat
    10561 tacgcacttc tcccgcaacc accgcaaaac cacgtccttg ctcacccgca cgcgctgctt
    10621 caaccgccgc gttcaaagcc agaatattgg tctggaaggt aatgccgtca attacgctga
    10681 taatatcggc gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat
    10741 ctaccacttt gccgccgcgt tgcgccgttt cagaagcact taatgccaga tggctggcct
    10801 ggcgcgcatt ctcggcgttc tgtttcaccg ttgcggtcag ttgctccatg ctggctgccg
    10861 tctcttccag cgaagcggcc tgttgctcgg tgcgcgaaga gagatcatta ttgccggtag
    10921 cgatttcgct ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac
    10981 gcatcagctc tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc
    11041 catccacctc aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca
    11101 ggcgattcat tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg
    11161 ccagtacgac gatcatcacg cccacaagaa tccacatcgc ctggctgtag gaggcattgt
    11221 tatcgctgac ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct
    11281 tctcgaaacc gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg
    11341 cacctaacag ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga
    11401 tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc
    11461 agtttttttc cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg
    11521 aaccgctacc aatattattc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt
    11581 tacgcgtctg caacaacgcg acccagctgc cattcagcgt ggattgctgc tggcgaatgg
    11641 tttgtaaaac agtgaaattt tctttgtcat tctttaaggc attaaaaaac agaccgcctg
    11701 atgtcagttg taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga
    11761 tacgttttaa catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc
    11821 aaaaacttta tgaaaaggtg taacccgcca tccatggaaa aaccaggcgc tggttatcag
    11881 atactaaaaa gttattcatt atcgcccggg atgtcttctt gtcgggaatt gcccagagca
    11941 ataaattcct ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca
    12001 atctgccgat aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct
    12061 ttggtcagcg agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa
    12121 ccgtcgcgtt ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga
    12181 tacgccagat cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac
    12241 tgttgctcag tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct
    12301 cgcgcctgga gcaacgcaat ggttagtgag tcgtgcatta tctttcccct gaaaaattca
    12361 aaatcattaa tatagaaaca attaatcatc acgttaccgg gactgagctg cccccgcaat
    12421 tcttcatttt atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta
    12481 tctttaaatg taaatagttt gttaattaga tcacatttat atcacttact tttgccaaac
    12541 tacgcaatcg ggaataaaag taatcattaa catattaatg attaagatga tcgaatccga
    12601 ggagtggtaa tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat
    12661 gcctggcagg aagcgttcca gcaatctccc tacaaagccc cgcctaaaac tgcggtctgg
    12721 tttattaaac cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt
    12781 gaaaaagtac tgagcggtgc gactgttgcg ctgattgtgg ggaaaacggc gacgaaagta
    12841 cgtgaagaag atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg
    12901 ccggaagaga gcttttaccg tccggcaatc aaagcaaaat gtcgtgatgg attctgcccc
    12961 attggcgaaa ccgtggctct cagcaatgtc gataatctga ctatctatac cgagatcaac
    13021 gggcgtcctg ccgatcactg gaataccgcc gatttacaac gtaacgccgc acagctgctg
    13081 agcgccctga gcgaatttgc cacgctgaat ccaggcgatg ctattctgct cggcacgcca
    13141 caggcgcgcg tggaaataca gccaagcgat cgcgtgcgtg ttctcgcaga aggtttcccg
    13201 ccgctggaaa atccggtagt ggacgaacgt gaagtgacca cgcgcaagag cttcccaacg
    13261 ctgccacacc cgcacggtac gctgtttgcc ctcggcctga actacgccga ccacgccagc
    13321 gaactggaat ttaagccacc ggaagagccg ctggtgttcc tgaaagcgcc gaataccctc
    13381 actggcgaca accagacctc cgtgcgcccg aacaatattg aatacatgca ctacgaagcg
    13441 gagctggtgg tagttattgg caagcaggcg cgtaacgtca gcgaagccga tgccatggat
    13501 tatgtcgcgg gctacaccgt gtgtaacgac tacgccattc gcgactatct ggaaaactac
    13561 taccgcccta acctgcgggt caaaagccgc gacggactga cgccgatgct ttcaaccatc
    13621 gtgccgaaag aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc
    13681 gagttacgcc agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc
    13741 tacttaagcg aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacaccaaaa
    13801 ggcttatctg acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc
    13861 ctggtgaacc gaattgtgag tgaggaaaca gcgaaatgaa aaaagtaaat cattggatca
    13921 acggcaaaaa tgttgcaggt aacgactact tccagactac caatccggca acgggtgaag
    13981 tgctgacgga tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga
    14041 aagaggcgtt cccgaaatgg gctgatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc
    14101 gtctgggcga tctgatcgac cagaacgtgc ctgagatcgc cgcgatggaa accgcggaca
    14161 cgggcctgcc gatccatcag accaaaaatg tgttgatccc acgcgcttct cacaactttg
    14221 aatttttcgc ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga
    14281 tgctcaacta cacgctggtg caaccggtag gcgtttgtgc gctggtgtcg ccgtggaacg
    14341 tgccgtttat gaccgccacc tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg
    14401 tgctgaagat gtcggaactc tccccgctga ccgccaaccg cttgggtgag ctggcgctgg
    14461 aagctggtat tccggcgggc gtgctaaacg tggtacaggg ctacggcgca accgcaggcg
    14521 atgcgctggt ccgtcatcat gacgtgcgtg ccgtgtcgtt caccggcggt accgccaccg
    14581 agcgcaacat catgaaaaac gccgggctga aaaaatactc catggagctg ggcggtaaat
    14641 caccggtgct gatttttgaa gatgccgata ttgagcgtgc gctggacgcc gccctgttca
    14701 ccatcttctc aatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcaacaga
    14761 gcatctatcc agaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtgggcg
    14821 atccgaacga tccgaatacc caggttaggg cgcttatcag tcagcaacac tgggaaaaag
    14881 tctccggcta tatccgtctc ggcattgaag aaggcgcaac cctgttggcg ggcggcccgg
    14941 ataaactgtc cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaacggtgc
    15001 tggcggacgt agataaccgt atgcgcgttg cccaggaaga gattttcggg ccggtcgcct
    15061 gcctgctgcc gtttaaagac gaagccgaag gcttacgcct ggcaaacgac gtggagtacg
    15121 gcctcgcgtc gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca
    15181 ttgaagcagg catggtgttc gtcaacaccc agaacgtgcg cgacctgcgc cagccatttg
    15241 gcggcgtaaa agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg
    15301 cggaaatgaa gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct
    15361 gatatgggta agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatctctct
    15421 gaactaccag gaaaaaacca cggttgtcgc cagggtgcga tcgacgggca taaagagatc
    15481 agcaagcgtt gccgggaaat gggcgtcgat accattatcg ttttcgatac tcactggctg
    15541 gtcaacagtg cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac
    15601 gagctgccgc attttattcg cgacatgacc tacaactacg agggcaaccc ggagttgggg
    15661 cagctaattg ccgaggaagc cttaaagctc ggcgtgcggg caaaagcgca caatattccc
    15721 agcctgaaac tggaatacgg cacgctggta ccgatgcgct acatgaatga agacaagcac
    15781 ttcaaagtgg tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag
    15841 ctgggcgaag cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc
    15901 agcggttcgt tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc
    15961 tacacccgcg agttcgaccg ccagatggac gagcgtgtgg tgaagctgtg gcgcgaaggc
    16021 cagttcaaag agttttgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc
    16081 aatatgcacg acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag
    16141 gtggagttta ttaccgaact gttcccaagc tctggcaccg gtcaggttaa cgctgttttc
    16201 ccgctgcccg cgtaaggacg ttttatgccg cactttatcg tggaatgtac tgaaaatatt
    16261 cgtgaagaag cccgcttacc tgagctgttt gccagtgtta acactgcact ggcggcaacg
    16321 gggatttttc cgcttggggg aatacgtagc cgggcgcact ggatcgatac ctggcaaatg
    16381 gctgatggca agcacgatta cgcctttgtg catatgacgc taaaaatcgg cagcggacgc
    16441 agtctggaaa gccgtcagga agtgggcgaa atgctgtttg atctgattaa aacgcacttc
    16501 gcctcactga tggagagccg ttacctagcg ctttcatttg agattgcaga gctgcacccg
    16561 acgttgaatt tcaaacagaa caacgtacac gcgttgttta aataaatttg caccagcttc
    16621 caggccggat gataaggcgc tgccgcatcc ggcaatgcca caggatatcg ctatgttcga
    16681 taaacacacc cacaccctga tcgcccagcg tctggatcag gcagaaaaac agcgcgaaca
    16741 gatccgcgcg atctcgctgg attacccgga gatcaccatc gaagacgcct acgccgtgca
    16801 gcgcgaatgg gttcgcctga aaatcgccga aggtcgcacg ctgaaaggcc acaaaatcgg
    16861 tctgacctcg aaagcgatgc aggcaagctc gcagatcagc gaaccggatt acggtgcact
    16921 gctggacgac atgttcttcc acgatggcag cgatatcccg accgatcgct ttatcgtgcc
    16981 gcgcattgaa gtggagctgg cttttgtgct ggcaaaaccg ctgcgtgggc caaactgcac
    17041 gctgttcgac gtttacaacg ccacggacta tgtgatcccg gcgctggagc tgatcgacgc
    17101 tcgctgccac aacatcgatc cggaaaccca gcgcccgcgc aaagtattcg acaccatttc
    17161 cgataacgcc gccaacgccg gagtgatcct cggaggtcgt ccgatcaaac cagacgaact
    17221 ggatctgcgt tggatctccg ccctgatgta tcgcaatggc gtgattgaag aaaccggcgt
    17281 cgccgctggt gtgctgaatc atccggcaaa cggcgtggcc tggctggcga acaaactcgc
    17341 accgtatgac gtacaactgg aagccgggca aatcattctc ggtggttcgt tcacccgccc
    17401 ggttccggcg cgtaagggcg acaccttcca cgtcgattac ggcaacatgg gctccatt
//
LOCUS       DADMEQ010000076        17383 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.077, whole genome shotgun sequence.
ACCESSION   DADMEQ010000076 DADMEQ010000000
VERSION     DADMEQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.077"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(40..771)
                     /gene="rlmB"
                     /locus_tag="JNZ05_002611"
     CDS             complement(40..771)
                     /gene="rlmB"
                     /locus_tag="JNZ05_002611"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAY8565796.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPMIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(951..3392)
                     /gene="rnr"
                     /locus_tag="JNZ05_002612"
     CDS             complement(951..3392)
                     /gene="rnr"
                     /locus_tag="JNZ05_002612"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAY8565797.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3431..3856)
                     /gene="nsrR"
                     /locus_tag="JNZ05_002613"
     CDS             complement(3431..3856)
                     /gene="nsrR"
                     /locus_tag="JNZ05_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HAY8565798.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLLE"
     gene            complement(4061..5359)
                     /gene="purA"
                     /locus_tag="JNZ05_002614"
     CDS             complement(4061..5359)
                     /gene="purA"
                     /locus_tag="JNZ05_002614"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAY8565799.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5463..5660)
                     /locus_tag="JNZ05_002615"
     CDS             complement(5463..5660)
                     /locus_tag="JNZ05_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HAY8565800.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5742..6746)
                     /gene="hflC"
                     /locus_tag="JNZ05_002616"
     CDS             complement(5742..6746)
                     /gene="hflC"
                     /locus_tag="JNZ05_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HAY8565801.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLRRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6749..8008)
                     /gene="hflK"
                     /locus_tag="JNZ05_002617"
     CDS             complement(6749..8008)
                     /gene="hflK"
                     /locus_tag="JNZ05_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HAY8565802.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8094..9374)
                     /gene="hflX"
                     /locus_tag="JNZ05_002618"
     CDS             complement(8094..9374)
                     /gene="hflX"
                     /locus_tag="JNZ05_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAY8565803.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9450..9758)
                     /gene="hfq"
                     /locus_tag="JNZ05_002619"
     CDS             complement(9450..9758)
                     /gene="hfq"
                     /locus_tag="JNZ05_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAY8565804.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9844..10794)
                     /gene="miaA"
                     /locus_tag="JNZ05_002620"
     CDS             complement(9844..10794)
                     /gene="miaA"
                     /locus_tag="JNZ05_002620"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAY8565805.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10787..12634)
                     /gene="mutL"
                     /locus_tag="JNZ05_002621"
     CDS             complement(10787..12634)
                     /gene="mutL"
                     /locus_tag="JNZ05_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAY8565806.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPCYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12644..13981)
                     /gene="amiB"
                     /locus_tag="JNZ05_002622"
     CDS             complement(12644..13981)
                     /gene="amiB"
                     /locus_tag="JNZ05_002622"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HAY8565807.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVLVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14000..14461)
                     /gene="tsaE"
                     /locus_tag="JNZ05_002623"
     CDS             complement(14000..14461)
                     /gene="tsaE"
                     /locus_tag="JNZ05_002623"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAY8565808.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14433..15980)
                     /gene="nnr"
                     /locus_tag="JNZ05_002624"
     CDS             complement(14433..15980)
                     /gene="nnr"
                     /locus_tag="JNZ05_002624"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAY8565809.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSVYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15979..17118
                     /gene="queG"
                     /locus_tag="JNZ05_002625"
     CDS             15979..17118
                     /gene="queG"
                     /locus_tag="JNZ05_002625"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAY8565810.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17101..17154)
                     /locus_tag="JNZ05_002626"
     CDS             complement(17101..17154)
                     /locus_tag="JNZ05_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565811.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3939 a         4956 c         4368 g         4120 t
ORIGIN      
        1 accatccaaa tctggatggc ttttcataat tcagaggaac tagctgcgct gacgaaccgc
       61 ttcaaataag caaattccgg ttgcaaccga aacgttcagg gaagaaacac ttcctgccat
      121 tgggatgctg atcaactcat cgcaatgttc acgggtcagg cgacgcatac cttcaccttc
      181 cgcgcccatc accagcgcca ggcgtccggt cattttgctc tgatagagtg tatgatccgc
      241 ctcgcctgcc gtaccgacga tccagatatt ctcttcctgc aacatacgca tggtgcgcgc
      301 aaggttagtc acccgaatca tcggaacgct ttctgccgcg ccgcaggcta cttttttcgc
      361 cgtagcgttg agctgtgcgg agcgatcttt cggcacaatc accgcatgaa cgcctgcggc
      421 gtccgcacta cgcaggcacg cgccgaggtt gtgcggatcg gttacgccgt cgaggatcag
      481 caggaacggt tgatcgagcg aagcgatcag atccggcaga tcgttttcct gatactgacg
      541 tcctggcttc acgcgggcga taatgccctg atgcacggca ccgtcgcttt tctcgtcgag
      601 atattggcgg tttgccaact ggataaccac gccctgagac tcaagggcgt gaatcagcgg
      661 taacagacgt ttatcttcac ggccttttaa aataaagact tcctgaaaac gttcaggggc
      721 gcgctccagc agggcctgca ctgcgtggat gccgtaaatc atttcgctca ttaatgtact
      781 cgttgtttac gttttgcctg atgcactacg tttatccggc ctacatgatc tctgcaatat
      841 attgaatttg cgtgattatg taggccggat aaggcgttca cgccgcatcc ggcatgaaca
      901 aagcgcattt tgtcagcaat ctaaccctct tcttttaaag agggtattga tcactctgcc
      961 acttttttct tcgccgcacg cttcgctttg gtcgctgcgg ctattttctg cgttttcgcc
     1021 gatggctttt tcgcttttct cgcgtctttc ttcgccgctt tcggcttcgt ttttttctca
     1081 ccgcggaagg cgctgtctgg ctcaaagttt acctttttac cgacctgacg acgcttgccg
     1141 ccttttttgc ctgcatcgcc ttttttcgct ttctcgcgcg ccgttttacc gacgttgcgc
     1201 ggtgcgcgtt cactggagat cagactaaag tcgattttgc gctcgtccat attaaccgct
     1261 tcgacgcgaa cttccacgcg atcgcccagg cgataagtct ggccgctgga ttcccccatc
     1321 aggcgttgcc ctacctggtc aaagcgatag tagtcattgt ccagcgaaga gacatggacc
     1381 agaccatcaa tgaacaagtc gtccagacgg acgaagaagc caaagccagt gacgctggaa
     1441 attacgcctt taaagacgtt acctacctgg tcgagcatga agtcacactt cagccagtca
     1501 gcgacatcgc gcgttgcttc gtcggcacga cgttccgcca tcgaacagtg ctgacccagt
     1561 tgcaacatct cttccatcga ataatggtag ccgccggttt cagtggtgtt gccctgatgc
     1621 ccctgctctt tcgccagcag atatttaatg gcgcggtgca gcgtcaggtc aggataacga
     1681 cgaatcggcg aagtaaagtg cgcataggac tgcaatgcca gaccgaagtg accacggttt
     1741 tctggatcgt aaatcgcctg tttcatcgag cgtagcagca tggtttgcag catttctgca
     1801 tcaggacggt cggcaaccga ctccagcagt tccgcgtagt cacgcggttc cggcttgtta
     1861 ccacccggca gctccagccc cagctccgcc agcactgaac ggaaagaggt aatcgcttcg
     1921 gtgctcggct tgtcgtgaat acggaacagt gccggttctt tcgctttctc aacgaaacgc
     1981 gccgccgaga tattcgccag aatcatgcac tcttcaatta atttgtgcgc gtcgttacgc
     2041 tgggtctgtt cgatacgttc aatacggcgt tcagcgttga aaatgaactt cgcttcttcg
     2101 ctctcaaatg agatcccacc gcgttcttca cgggctttat ccagcacttt atagaggtta
     2161 tgcaactctt cgagatgctt aaccagcggg gcgtactgct cgcgcagatc ctgatcgccc
     2221 tgcagaatat gccagacttt ggtgtaggtc agacgcgcgt gagagctcat caccgcttcg
     2281 tagaatttgt agcccgtcag gcggcctttc gacgaaaccg tcatctcgca caccatacac
     2341 aggcggtcta cctgcgggtt gagcgaacac aggccgttag agagcacttc cggcagcatc
     2401 gggataacct gcgaagggaa gtacaccgac gtgccacggt tacgcgcttc tctgtccagc
     2461 ggcgttggcg gacgcacata gtagctgacg tcggcaatcg cgacccataa acgccagccg
     2521 ccgccgcgtt ttttctcgca gtaaactgca tcgtcaaagt cacgggcgtc ttcgccatca
     2581 atggtgacca gcggtaaatc gcgcaaatcg acacggcccg cttttgcttc ttccggcact
     2641 tcttctttca gccccgcaac ctgttgctca acagcctgcg gccagatata cggaatttca
     2701 tgggtacgca gagcgatatc aaccgccatg ccggtgccca tattgtcgcc cagcacttcg
     2761 acgattttac ccaccgcttt ggtgcggcga gtcggacgct gagtcagttc gactacgacc
     2821 acaaagccca tccgcgcgcc catgatctga tcgggcggga ttaagatatc gaagctcaga
     2881 cggctatcgt caggaaccac aaagccgacg cccgcttcgg taaagtagcg accaacaatc
     2941 tggctggttt ttggcaccag tacgcggaca atacgcgctt cacgacgacc tttacggtca
     3001 gcacccagcg gctgcgccag cacctgatcg ccatgaatgc aggttttcat ctgctcgctg
     3061 gagagataca aatcatcttt acgcccttca acccgcagaa agccgtagcc atcacggtgg
     3121 ccaataacgg tacctttcac caggtcgagg cgttccggca gcgcatagca ctgacgacga
     3181 gtgaagacca gttgaccatc gcgctccatc gcgcgcaggc ggcgacgcag gccttcaagc
     3241 tgctcttcgc cttcaatgtg cagttctacc gccagctcat cacggctggc cggtttttca
     3301 cgtttggtta aatgttcgag gataaattcc cgactaggga tgggattcgc gtatttttca
     3361 gcttcgcgtt cctggaaagg atcttgtgac atctcggttc ctccgttgtc atctctgatg
     3421 aagattttcg tcactccaac agcaataatt tataaagcgg ttgattctct tcaaccaaat
     3481 cggcaagcgt gtagttatcc agttccgtaa gaaaactttg cacggcctta gaaagcgcct
     3541 gtttcaaccg acaggcaggt gtaatgtggc aaaactcact gctgcaattc accagcgata
     3601 agggctccag ctcgcgcacc acatcaccaa tacgtatcgc actcgccggt ttacccaggc
     3661 gaatgccgcc attttttcca cgaacagcag tcacgtagcc ggcacgacta agttgattga
     3721 ttattttgac catatgatta cgggagacgc cgtagacgtc agtcacttca gaaatactgg
     3781 tcatccgccc ttctggcaat gacgccatgt agatcagcgc acgtaatccg taatcagtga
     3841 aactcgttaa ctgcacatca acctcaaaag ggaaatcggg aaaaaataca tttatattga
     3901 tgataaacca gccacaagct gtgtcgctaa tttatttcag ttcggggaag gaaaaaagcg
     3961 aggatttaac actgtgtcgg atagcgtaat ggcttaaccg acacagcaat gtatcacact
     4021 gttagcccgg caggcaaaat atctgccagg cgtaccagaa ttacgcgtcg aacgggtcgc
     4081 gcagaatcat ggtttcagta cgatccggac cggtagagat gatatcgatc ggcacaccgg
     4141 tcagctcttc aatacgcttg atgtagttca gcgccgcctg cggcaggccg ctacgatctt
     4201 tcacgccgaa agtggattca gaccagcccg gcatggtttc gtaaatcggc tctacacctt
     4261 tccagtcgtc agctgccagc ggagtggtag tcacttcgcg accatccggc atacggtaag
     4321 ccacgcagag tttaacctct ttcaggccat ccagaacgtc cagtttagtc aggcagaagc
     4381 cagacaggga gttcagctgt accgcacgac gaacggcaac ggtgtccagc cagccggtac
     4441 gacgacgacg acccgtagtt gcaccgaatt cgttaccctg cttgcagagg aactcgccag
     4501 tttcatcaaa cagttcggcc gggaaaggac ctgcacctac acgagttgag taagctttga
     4561 ggatacccag aacgtaatca acataacgcg ggcccaggcc ggaaccggtc gccacgccac
     4621 cagcagtggt gttggaagaa gttacgtacg gataagtacc gtggtcgata tccagcagcg
     4681 taccctgcgc accttcgaac atgacgaaat cgccacgctg acgcgcctgg tcgagcaggt
     4741 cagaaacgtc aaccaccata gaagtcagga tgtcggcaac agccatcgta tcatccagaa
     4801 ctttctggta atcaaccgct tcagctttgt agtagttaac caactggaag ttgtgatatt
     4861 ccatcacttc tttcagtttt tcagcgaagg tttctttgtc gaaaaggtcg ccaacacgca
     4921 gaccgcgacg tgccacttta tcttcataag caggcccgat accacgaccg gtggtgccga
     4981 tcgctttcgc gccacgcgct ttctcacgcg cgttatccag cgcaacgtga taatcaagga
     5041 tcagcggaca tgcttcagac agcagcagac gctcacgaac ggggatgcca cggtcttcca
     5101 gttctttcat ctctttcatc agcgcggccg gagacagcac aacaccgtta ccgatgatgc
     5161 tggttacatt ctcgcggaga atacctgatg gaataagatg gagaacggtt ttttcaccgt
     5221 tgattacgag agtatggcct gcgttgtgac cgccctggta gcgtacaaca tatttagccc
     5281 gttcagtcag aagatcgacg atcttacctt taccttcgtc accccattgg gtgcccagta
     5341 cgacgacgtt gttacccatt tttcaaaatc accgtttgct taaaaatgga ttctaccatc
     5401 gctttttcag agttacagca ctttttgcac tcaaaaatga cctcgaacag tctgtttttt
     5461 gttcagccaa tcgttttcct caacatgtag tagaccacaa cgcccgcaac cacaagtcca
     5521 ccgccaaaac gacgtaaaat attatcgggc aaattggtca tcgcagagat catcttcttc
     5581 catgccttcg ggtaaagcat cggccctaaa ccttccagta ccaaaaccag ggcaagcgcc
     5641 agccagattg tcgaattcat tcattatcct tatagaaaaa gaaaaccacc gacatccctg
     5701 aggatgcggt ggctttattg acctgtaccg cagtcgttat attaacgcgt tgcggaagtc
     5761 ggcgtcttca tgtagcggaa gaaatcgcta tccgggctca tgaccatcac gtcctgattg
     5821 ccagagaagc tgttctcata agcacgcagg ctacggatga atgcgtagaa gtccggatct
     5881 ttactgaatg catcagcaaa cagtttggct gcttcggcat cgccttcacc acgcataatg
     5941 cggccctgac gctcagcttc tgccagcgtt ctggtcactt catagtcggc agtcgcgcgc
     6001 agtttttccg cttcttcctg accttgtgaa cggtgacgac gcgctaccgc ttcacgctcg
     6061 gcgcgcatac ggttgtagat cgcttcagac acttcggtcg gcaggttgat ctgcttgata
     6121 cgcacatcga caacttcaat acccagcgcc gccatactgt tcgggttgat gaccggaact
     6181 ttgcccttcg tctcagccgt tacgcgctct gccgcttcgg caatggcgtt atctgctgcc
     6241 ggggtagtaa cttcatcttc tgtacccgca gaaccggagt tcagcgcgtc acgtacttcg
     6301 agggtcagac gaccacggga atcggtgacg atatctttca cgtccaggcg accaatttca
     6361 gaacgcagac ggtcagagaa cttacgtctc aacagcactt ccgcttgcga aatgtcgcca
     6421 ccacccgttg ccaggtagta acggctgaaa tcgctgatgc gccatttgat gtaagagtcg
     6481 acgatcaggt ctttcttctc tttggtcaca aagcggtcgg cctggttgtc catggtctga
     6541 atacgtgcgt cgagcatttt caccgtttca atgaacggta tcttgaaatg cagaccaggc
     6601 tcataaacca aaggtttgtt gtcatcgtca cgcagtacct taccaaaacg cagcgtaata
     6661 ccgcgctcac cttctttgac gacaaagaca gacatgtaaa gcactaccag cacgatgatg
     6721 ataatcgcga taactgactt acgcatcgtt attccccctg acgctggtag tcgttacgct
     6781 gcgcgttggc gcggcgttgg tccataatat cgccctgact ggtggacgac gtgttgcttg
     6841 ctccactggt tgtggaggaa gaggctggcg gcagacgcag cagattgctg gcaccgttat
     6901 cgctcttcgc cgcaggggcg ttaccacctt tcaacatctg gtctaacggc agaaccatca
     6961 ggttgccacc tttatcgtta accagcactt tgcgggtgtt acccaacact ttttccatcg
     7021 tctcgatata cagacgctcg cgagtaattt ccggcgcggc tttatattcc ggcagaagtt
     7081 tagcaaagcg cgccacttca ccctgagctt ccaggatggt ctgggccttg tacgcacgcg
     7141 cctcttcgag gatacgttgc gcctgaccgt tcgcacgcgg ctgaacttcg ttggtatacg
     7201 cttctgcttc acgaatgtat tgctgttcgt tttcacgcgc ggcaatcgca tcgtcaaacg
     7261 ccgcttttac ttcttccggc ggacgagcag cctggaagtt gacgtccagc agcgtgatac
     7321 ccatgtcata cggacgaatc gtctcttcca gttcgcgctg agtatcgcta cgaatcacgg
     7381 tacgaccttc cgtcagaatg cggtccatgg tgtatttacc gataactcca cgcagggcgc
     7441 tgtcggtagc ctgacgcagg ctgtcatccg ggctggtcac gctatacaga tatttttccg
     7501 gattggtgac gcggtactgc acgttcatct caacgcgcac tacgttctcg tccgacgtca
     7561 gcatcacacc agaagcggcc agttcacgca cggcttccac gttcaccggt ttgacttcgt
     7621 cgataaacgt cggtttccag ttcagacccg gctcaaccag atggctgaat ttaccaaagc
     7681 gtgttaccac gccgcgttcg gcttctttaa tggtatagaa accactggcc gcccagataa
     7741 tgacaatcgc tgccgctgcg atggtaacga cacgaccgcc aagctgcggg cgcgggcctt
     7801 gcgatgaact gccaccgcca gatccggtgc ctttaccgcc gcccagacca ccgagctttt
     7861 tgctcagttt gcggaagata tcatctaaat caggtggccc ttgatcgcga ccgcctttgt
     7921 ttccatttcc ctcagagttg ccgccaggtt tgctgcttcc ccacgggtcg cggtcttgtc
     7981 cgttattacc gggctgattc cacgccatgt ttgtgctcca tatttgttat gcggtgatcc
     8041 ctgttgtctt tcgacgccag aggatatgac tccacgcttc agacgctacg ccgttagatc
     8101 aggtaatcga tcaacgccgg ttcttgttta cagaggcgac gccagtcaac gatcggcata
     8161 cgaacttgca gacttacgct gccgtcctcc tccatccact ctttttctat tgcctgaagc
     8221 tgataaaaac gactcctcag acgcccttcc tgcggtggca gacgcaatgt atgctgcgcc
     8281 acctcgccgg aaagccgctc cgtcaaagcc tgaaaaagct gtggtatccc cgctccggtc
     8341 tgtgcggaaa gccagacacg gatcggtttg ttctcttcgt cccgatcaat acgcggttcg
     8401 aaatcttcca gcatatcgat cttgttcatc accagcaggg ttgggatctc gtgagcgtcg
     8461 atctcttcaa gaaccgtatt caccgcttcg atgttttctt gtacacgcac atccgccgca
     8521 tcaatgacgt gcagcagtaa tgtggcttgc cgcgtctctt gtaacgtggc tttaaatgct
     8581 gccaccagat cgtgcggcag gtggcgaata aaccctacgg tatctgcaag tacggtttca
     8641 ccgacatctg caacgtcaat acgccgcaac gtcgggtcga gggtggcaaa caactggtct
     8701 gccgcgtaga cccgcgcttc ggtgatgcga ttgaaaaggg tagatttacc ggcgttggta
     8761 tatcccacca gcgaaacagt aggaacgtcg gctttgatac gcgattgccg cccctgctca
     8821 cgctgctttt caactctttc caggcgcgac tgtatctgca cgatgcgatt acgcaacaaa
     8881 cgacggtcgg tttcgagctg ggtttcaccc ggaccacgca aacctatccc gcctttctgt
     8941 ctttcaaggt gggtccagcc acgcaccagg cgcgtagcca gatggcgcag ctgcgccagc
     9001 tcaacctgca acttaccctc atgggtacgc gcacgttggg cgaaaatatc taaaataagg
     9061 ccggtgcggt cgataacacg acactcgcac aaacgctcca ggttacgctc ttgcgccggg
     9121 ctcagggcat ggtcaaaaag aacgaccgaa gcacccgtcg ctttgacagc ttccgcaatt
     9181 tcaactgctt taccttcacc tacaaaatac tttgggtgcg gcgctttacg gctaccggta
     9241 atcacctgca atgcttcgac accggcggaa gagaccagag attcaaactc ctggaggtct
     9301 tccatatctt tgtcttgcgt aaaatagatg tgtaccagta ccgcctgctc accagcatca
     9361 taacggtcaa acaagcgtat aaccctctaa atagatcagc ggggaacgca ggatcgctgg
     9421 ctccccgtgt aaaaaaacag cccgaaacct tattcggttt cttcgctgtc ctgttgcgcg
     9481 gaagtattct gtgcgctgct accatgatgg tagttactgc tggtaccgcc accggcgttg
     9541 ttactgtgat gagaaaccgg gcgagatggg acaacagtag aaatcgcgtg cttgtaaacc
     9601 atctggctga ccgtgttttt caacaggatc acgaactgat caaaagactc gatttgccct
     9661 tgcagcttaa taccattcac caaataaata gaaactggaa cacgttcccg acgcagtgcg
     9721 ttcaggaacg gatcttgtaa agattgcccc ttagccattc tctcttttcc ttatatgctt
     9781 atttgtactt tgaacctttc gattctgaaa aaattgcgca cgatacgtct caattgtaca
     9841 cattcagcct gcgatagcac caacaacctg taatacttcg tcacgcgcct gttctggttt
     9901 ttcactgtca agccagtgaa ccccttccca accacgcagc caggttatct gccgcttcgc
     9961 caactgtctc gtggcgcaaa cacctcgata aaccatttca tcgtatgaga tttcgccttc
    10021 aaggtaagac cacatctggc gataacccac gcaacgaatg gaaggcaagt ccgtatgcaa
    10081 atctcctcgg gcaaaaagcg cccggacttc tgcttcaaaa cctgaagcca acatctgatg
    10141 aaaacgctgc tcaatgcgtt gatggagcag ttcacggctc gccggggcga tggcgaactg
    10201 atgcacctga tacggtagag cgtctcctga cgtttgcgtc agttccgtta aagttttacc
    10261 cgaaatgaaa aaaacttcca gtgcccggga aagcctttgt ggatcatttg gatgaatcct
    10321 tgctgccgca accggatcta cctcctgaag ttgacgatgc aatgactccc aaccttgctc
    10381 tgccgcctgt tgctcaattc tggcccgcac ttccgggtct gccgacggta gcggcgacaa
    10441 cccttccagc aatgccttga aatacaacat cgtaccgccc actaacagtg ggatccgccc
    10501 cgccgcggtg atatcggcca tttccgccag cgcatcgcgg cgaaaatcag cagccgagta
    10561 agcctgcgac ggatcgcgaa tatccagcaa tcggtgcggc gcggcgagta actcttcagc
    10621 gttcggcttc gccgtcccga tatccatccc tttgtaaata agggcagaat caacgcttat
    10681 caactctact ggtaaaattt tacgcagctc aatggctaac gccgttttac cggaggccgt
    10741 cggccccatc aaaaaaatcg ccttaggcag gctcgcctta ctgatatcac tcatctttca
    10801 gggcttttat cgccggatgt aaatcaacag attgtaacag accacccggc ggcgttttca
    10861 caagttgcgg acataaccgt tccacgtccg ccagcagggt tatggcctgt gccattgacc
    10921 actgcgcatg ttcgctcatc agatttcgtg caatccactg cgcaatattg ccaggttcga
    10981 atacggactg cttcgccagg tagcctatca gttcaggaat caagatttgt aaattttgtt
    11041 ggcgtaaggg taaaggcacg gccctgatgg tcacatgctg tgcatctgac tggaaatcaa
    11101 tacccaattc cgctagggca gactgcgctt tttctaatgc cgatttttct tcgccagaaa
    11161 cttttagccg caacggaatc agcagcggct gggcgcaaac gggcgcttca cccggcgtca
    11221 attgtgcctg acgcagccaa cgttctgcca ctggcaagga taaaagtgaa atgttgccat
    11281 cgcgctccag caacgcacag tcggaatgga cgatagtcag tacccgacca aaactctgac
    11341 tgttcgccgc aagtgcaggt tcctgcggtt ccggcgcttt tggtttttgc atcggcgcgg
    11401 gcgtttgcaa aagctggcga tacacttcac cttgctgttt ctggtagcct ggctgcgcat
    11461 tcggccaggg ggcagccgga cgactgcctg aggcgggcgc aggagagtag cacggagcta
    11521 ccggctcacg agctgccggt tctgcaaagt gattgcgccc cgccgccacg cggttttccg
    11581 ggatggcacg cggtgcgggt tgtggttcat cgtccagcgg tagcggcgtt tccagttgct
    11641 gttgtagcac gctcagcacg ccctgataga taaaatcatg caccagacgc gactgatgga
    11701 aacgcacttc gtgtttggcg gggtgtacgt tgacgtccac ctgatgcggg tcgatctcca
    11761 gatacaacac aaatgccggt tgctgatcgg cccccagttt gtcttcgcag gcctggcgga
    11821 tcgcatgatt gatcaggcga tcgcgcatca tgcgaccatt cacgtagcaa tactgaattt
    11881 ccgccagtgc gggcgtagtg tgatttggat cggccaccca gccgcgcagg gtgagatcgc
    11941 cgtgttgcca ttcaatcgcc agcgcttgtt cgagaaaagc ggtgccgcaa atcgcgccta
    12001 agcgccgttc tttttgcccg ccttccggca ctgcgcggta ctgacgcaca attttaccgt
    12061 tatgcgacag gttgatcgtg acgtcgaaac gcgccagcgc aatgcggcgg atgatttcat
    12121 caatatggct aaattcggtt ttctcggtgc gcaggaattt gcgccgcgcc ggggtgttgt
    12181 agaacagatc cagcacctcc agcgtcgttc ccaccggatg cgccgccggt ttaaccgtca
    12241 cgtccatgtc gcgtccttcg gcataggcct gccaggcttc ctgctgttct gcggtgcgtg
    12301 aagtgagcgt caggcgggaa accgaactga tactcgccag cgcctcaccg cgaaagccca
    12361 ggctgataat ggcttcgaga tcgtccagag aggcgatttt actggtggca tgacgagcca
    12421 gcgccagcgc cagctcatct tttttgatac cgcagccgtt atcacgaatg cggataagtt
    12481 tcgccccacc gcgttcgata tcaatatcga tacgcgtcgc acctgcatcg aggctgtttt
    12541 ccactagttc tttgactacc gacgcaggtc gctcgaccac ctcacctgcg gcaatctggt
    12601 tcgccagttg tggcggtaag acctgaattg gcatcaatcg tccttagttt ggcagcgtgc
    12661 gatctggcgt cgtcaccgta ctggcagttt gtgccgttgc accctgcggc gcagattgca
    12721 tcggatgcgc aaggaagtaa ttgcgcaggc ctttgtaaat ggcttctgcc agctgttgtt
    12781 ggtaatcgtc gctcgccagc aaacgttctt cgctgttgtt gctgataaaa ccggtttcga
    12841 ccagtactga tgggatatcc ggcgagcgca gaacgccaag gctggcgtgt tctggtcgac
    12901 gtttatgtat ttcgccaatg cgttgcaact gactgatcat actggtcgct acatcatacc
    12961 ctacccgctg ggaatgaccg aactgtaaat ccagcaccgc ctggcttaaa taggggtcag
    13021 actgactgtt cgccagcaca tcacccgccc cacccagcag ctccgactgt ttctcgtgct
    13081 gctccagcca gctggccatt tcactgttgg cgcgacggtt agagagcacc aatacggaag
    13141 cgccagtcgc actgcggttc ggtgcggcat cagcgtgaat cgacacgagg aaattggcgt
    13201 tttgcttatg tgccacatca ctgcgcccca tcaccgagat aaagtaatcc ccgtcacggg
    13261 ttaaaacgcc tttaaacatc ggatcgtcat tgagcaaagt acgcaattta cgcgcgatgg
    13321 cgatggtgac atttttctcc cgcgtaccac cggggccgat agcgccaggg tcctgaccgc
    13381 cgtgtccggc atcaatagcg atgataattt tatcgccagt gttagccgtc gcgcgcgctg
    13441 ccggacgcgt taccgtatta ctgctgataa cacccgtagt gcggttactt tccgttttaa
    13501 acggattgcg cgccggttcg ctgacgcgcg gtgcgacaac cgcaggcgtt tcaacgcgtt
    13561 tcgcaaccac gggcggcgga ggaggcggtg gcggcacatc ggcgttaatc gtaaagacga
    13621 cagtgtaatt gctgccattc tgccgcttca ccgcttcggt tttaccgttt tcggtaagat
    13681 cgaccaccag ccgtagcgtt tgtgcatctt taggcgttcc agagcgaatc gccttcacca
    13741 gattattgcc gctgaacaac aacggcagtc cctgaatcac gcccgtttgt ttgatatcga
    13801 gcgccacggt gcgtttgctt tgatggctaa acgcataatc aggatcgcca ataaaactca
    13861 acgttatccg tgcctgttga ttaccgttag aaacctgaat atcagagagc gtcgcggcac
    13921 ccaccggcgt gcacagcagc agcagcgtcg ctaccaacca atttctgatg cgatacatca
    13981 tcccgccacc tttcaaaggt taaccggcta aacgcgccag caacaattca cccgcagagg
    14041 aaaccgcact cacgcgcgcc tcacggcctt gtgcctgata atcaatgtgt atttcgacat
    14101 ccgggtcagg aagaacacct gtaccttgtt gtggccactc cacaaggcag atggcatcgt
    14161 tggcaaaata atcgcggatc cccataaact ccagctcctc gggatcggca aggcggtaca
    14221 aatcaaagtg atagaccatt aagttgtcga gcgtataggg ttcgaccagc gtataagtgg
    14281 ggcttttgac attaccctga tgacccagag cctgtaaaaa gccccggcta aaggtggttt
    14341 tacctgcgcc taaatcgcca tacagataga ttacggttgc tccatcgcag gctttcgcta
    14401 cccgctcgcc caggtctaat gttgcctgct catcagggag cggaattact cgattcatca
    14461 tggtttttat cagtcacttc cgggttaaca atacgctgta gcgtggaaaa gagatcggtt
    14521 gccagcatcc cgcgcgttcc aaaacgcgcc gccagtacgt cagctgccgc accgtgcgcg
    14581 acacagcctg cacaggctgc atcatacggc gacagttttt gcccaagcaa tgcgccaata
    14641 ataccagaga gcacatcgcc catgccgccg ctcgccatgc ctgcatttcc ggcatcaata
    14701 atgcctaaag cgtcaggatg ggcggcgacc acggttccgg cacctttcag caccgctacg
    14761 ccgccataac gttgtaccag acgtttggcg caatgtaagc ggtcactttc aatttcagcg
    14821 acggaacagc ctaacaaccg tgcggcctcg ctaggatgcg gcgtgatcac gcgattgtga
    14881 cgcttatcgg gattgattgc cagcaggttc aatgcatcgg catcccacaa catcggtttg
    14941 cgaaaattct caactttttg cagggctttt ttcccccact cttgctggcc cagaccggga
    15001 ccaatcacca ccacatcggc ccattccagg ctttcggtaa gagagtccat agtcagttca
    15061 tgcaccatca attctggtcg tgcagtcagc agcggcgcaa tgttctcact gcgggtcagt
    15121 actcggacta aaccagcacc agcacgtagc gccgcttccc ccgtcatacg aatagccccc
    15181 gccgtgccgt gatcgccacc gataatcacc agccgcccgt gatcgccttt atgcgaagtc
    15241 gggcgacgcg gtttcagcca gtgagaaagt tgttctgccg aaaaccgctg aattttcgtc
    15301 tcctgacctg ccagccagct atccagcccc agtgagtcaa aatgcagttg tcctgtaaca
    15361 tcccgcgctt ttccagtgag caagcctagt ttcagcgcaa taaaagtgat ggtgtgatcg
    15421 gcgttgatca ctgcgcctgg cgtagcgccg gtttcagcca gcaggccgga agggatatca
    15481 accgccacaa tcggcgcagg atgggaatta gcgtggtcga ttaactggct aatggattcg
    15541 cggggcgctt gctgcaagcc ggtaccgagc agcgcatcaa caatcagatc taccgattcg
    15601 ggccagacaa tattcgaagc atggatctcg ccacccgcgt ttaaccatgc ttcgcgtgcc
    15661 agcgcggcct cttccggcaa cggtttgtcg ctctcctggg ccaacaacgt gacctcaatg
    15721 ccgaccgctt tggccagtcg cgcgaccacg tagccatcgc cgccgttatt accatgaccg
    15781 cacagcacca gccagtggcg ggcgtcagga tacaccgaac gacacacctg gaatgcggcc
    15841 tcgccagcgc gaagcatcag ctcatagagt gtgagcccca gcacatctgc cgcctcgcgt
    15901 tctccgcggc ggatatcgtc ggcgtaccag acggtgtgtg gtatacttac ggggtttttc
    15961 ttcattgtat ggtccgtcat gtcagagccc ctcgatctca atcagttagc gcaaaaaatt
    16021 aaacagtggg ggctggaact gggctttcag caggtaggta ttaccgatac cgatctcagc
    16081 gagtccgagc ccaaactgca agcatggctg gacaaacaat accacggcga aatggactgg
    16141 atggcacgtc acggtatgct gcgcgctcgc ccccatgagt tattacccgg tacgctgcgt
    16201 gtaatcagcg tgcggatgaa ttaccttcct gctaacgccg catttgccag cacgctgaaa
    16261 aaccccaaac tcggctatgt tagccgttat gcgctgggcc gtgactatca caaacttctg
    16321 cgcaaccgac tcaaaaagct gggcgagatg attcagcaac attgtgtttc gctgaatttt
    16381 agaccgtttg tcgattctgc gcctattctc gagcgcccgt tagctgaaaa agctgggctc
    16441 ggctggacag gtaagcactc acttatcctc aatcgcgagg ccggttcgtt cttcttttta
    16501 ggcgaattgc tggtcgatat tccgctgccc gtggatcaac cagtcgagga aggatgcggc
    16561 aaatgcgtgg cctgtatgac gatttgcccg accggtgcca tcgtcgagcc atataccgtc
    16621 gatgctcgcc gctgtatctc ttatctcacc atcgaacttg aaggggcgat cccggaagag
    16681 ttgcgaccgt taatgggaaa ccgtatttac ggttgcgatg actgccagct tatctgcccg
    16741 tggaatcgct attcgcaact cactacagaa gacgatttca gcccgcgtaa gccgctacac
    16801 gcaccggaac tcattgagtt atttgcctgg agcgaagaga agtttttaaa agtcacggaa
    16861 ggatcggcga ttcgccgtat aggtcacctg cgctggctgc gtaatatcgc cgtggcatta
    16921 ggcaatgccc cttgggatga aacgattttg acagcgctgg aaagtcgtaa aggtgagcac
    16981 ccacttctcg atgagcacat agcgtgggcg attgcgcagc aaatagagag acgaaatgcg
    17041 tgcatagtcg aagtgcaatt gccgaaaaaa cagcgtctgg ttcgggtgat tgaaaaaggg
    17101 ttaccgcgtg acgcctgatt catccacagc ttgtgaataa aaataaaaac gcattgcaat
    17161 tcaagacgaa taaaatcgtc aagtgatcga ctcgacaaaa cggaatcttg atttttaact
    17221 tatttatcaa taggttaaaa aataccattc accgtgcgaa gtttctttga aattaagatt
    17281 acagcctgag gctgtggata actctgttta caagagtttg tcagagacaa caaaaaccag
    17341 caataacgca tcgcgtcgct gtggatattt tattgagaga aga
//
LOCUS       DADMEQ010000077        17341 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.078, whole genome shotgun sequence.
ACCESSION   DADMEQ010000077 DADMEQ010000000
VERSION     DADMEQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.078"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(267..1295)
                     /gene="epmB"
                     /locus_tag="JNZ05_002627"
     CDS             complement(267..1295)
                     /gene="epmB"
                     /locus_tag="JNZ05_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY8565812.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1337..1903
                     /gene="efp"
                     /locus_tag="JNZ05_002628"
     CDS             1337..1903
                     /gene="efp"
                     /locus_tag="JNZ05_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY8565813.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1955..2080
                     /gene="ecnA"
                     /locus_tag="JNZ05_002629"
     CDS             1955..2080
                     /gene="ecnA"
                     /locus_tag="JNZ05_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY8565814.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            2191..2337
                     /gene="ecnB"
                     /locus_tag="JNZ05_002630"
     CDS             2191..2337
                     /gene="ecnB"
                     /locus_tag="JNZ05_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY8565815.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2513..2742
                     /locus_tag="JNZ05_002631"
                     /pseudo
     CDS             2513..2742
                     /locus_tag="JNZ05_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(2739..3272)
                     /locus_tag="JNZ05_002632"
     CDS             complement(2739..3272)
                     /locus_tag="JNZ05_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY8565816.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3361..4494)
                     /gene="blaEC"
                     /locus_tag="JNZ05_002633"
     CDS             complement(3361..4494)
                     /gene="blaEC"
                     /locus_tag="JNZ05_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY8565817.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEKKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            complement(4557..4916)
                     /gene="frdD"
                     /locus_tag="JNZ05_002634"
     CDS             complement(4557..4916)
                     /gene="frdD"
                     /locus_tag="JNZ05_002634"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY8565818.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4927..5322)
                     /gene="frdC"
                     /locus_tag="JNZ05_002635"
     CDS             complement(4927..5322)
                     /gene="frdC"
                     /locus_tag="JNZ05_002635"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY8565819.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5333..6067)
                     /locus_tag="JNZ05_002636"
     CDS             complement(5333..6067)
                     /locus_tag="JNZ05_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY8565820.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6060..7868)
                     /gene="frdA"
                     /locus_tag="JNZ05_002637"
     CDS             complement(6060..7868)
                     /gene="frdA"
                     /locus_tag="JNZ05_002637"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAY8565821.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTCLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            8193..9170
                     /gene="epmA"
                     /locus_tag="JNZ05_002638"
     CDS             8193..9170
                     /gene="epmA"
                     /locus_tag="JNZ05_002638"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY8565822.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEANIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9389..10891
                     /gene="yjeM"
                     /locus_tag="JNZ05_002639"
     CDS             9389..10891
                     /gene="yjeM"
                     /locus_tag="JNZ05_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HAY8565823.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(11042..14353)
                     /gene="mscM"
                     /locus_tag="JNZ05_002640"
     CDS             complement(11042..14353)
                     /gene="mscM"
                     /locus_tag="JNZ05_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAY8565824.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQGLRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALRSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(14375..15343)
                     /gene="psd"
                     /locus_tag="JNZ05_002641"
     CDS             complement(14375..15343)
                     /gene="psd"
                     /locus_tag="JNZ05_002641"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAY8565825.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDARPLVDDKKDQV"
     gene            complement(15440..16492)
                     /gene="rsgA"
                     /locus_tag="JNZ05_002642"
     CDS             complement(15440..16492)
                     /gene="rsgA"
                     /locus_tag="JNZ05_002642"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAY8565826.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            16587..17132
                     /gene="orn"
                     /locus_tag="JNZ05_002643"
     CDS             16587..17132
                     /gene="orn"
                     /locus_tag="JNZ05_002643"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAY8565827.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         4003 a         4469 c         4545 g         4324 t
ORIGIN      
        1 aaaggttaat tattggtgtt agctatataa aaagtaacaa cagcaatgca tatgaataat
       61 taaaggttat gcaacgtgca aagattaaat ttctcagttt aatgacaggt tgctgtactt
      121 gcgtttgttg ctcttgccgg atgcggcgta aacgccttat ccggcctaca gttcattgca
      181 aatccattca atttcaatgc acgatgtagg cctgataagc gtagcgcatc aggcaatttt
      241 aatgtttaac ttccctgttt aatcagttac tgctggcgta gctggagatc cagcggcgtt
      301 ttgctgggtt cgccgccaat ttctcgcgcc agtttcggca ccagatatcc cgacaccaat
      361 gtcagcaact cacgcataat ctgccgtgct tcgtcatcac tcaccataaa atgcgccgcg
      421 ccctgtactt tatctagcac atgcaggtaa tacggcatca cgccggcatc gaacaacgca
      481 ttactcaggt ttgccaacgt ttgtgcgtta tcgttcacac cacgtaacag aacgctctgg
      541 ttaagcaggg tgacaccgac acggcgcaat ttagccatcg cctgacggaa cgtttcatct
      601 acctcattgg catgattgat gtgattcacc agcaagattt gcagcgtaga acgggcaaag
      661 cgttcaacca gcgcctcggt gatacgtgcc gggatcacaa tcggcagacg gctgtgaatc
      721 cgcagacgtt tgatatgcgg gatggcttcc agttgtgtga gcaaccagtc cagctcgtga
      781 tctttcgcca tcagcggatc gccgccggag aaaatcatct cgtccagttc cggatgcgca
      841 gcaacatact caagtgcagt ctgccagtta cgcttgttgc cctgattttc ggcatagggg
      901 aagtggcgac ggaagcaata gcggcaattt accgcgcagc cgcctttgac cagcaaaagc
      961 gcccggttgt ggtatttatg caacaaacca ggcactacgc tgtgctgttc ttccagtggg
     1021 tcggtggaga atccggacgc gacgacaaac tcatcttgcg aggtaagtac ctgacgcaaa
     1081 agaggatcgt ccggattgcc tttctccatg cgatcgataa atgagcgggg cacacgcagg
     1141 gcaaacagct ttttggcgct gcgtccggcc aacagttttt cgtccgcgtc tatattcaaa
     1201 agacgcagaa gttcatcagg atcggtcaca acatcggcaa gttgcgttaa ccaatcttct
     1261 ctggatgggg tatttagggt tacaatatgc gccattttgt ggcttagcta ccaattaaca
     1321 aatttcagag ggccttatgg caacgtacta tagcaacgat tttcgtgctg gtcttaaaat
     1381 catgttagac ggcgaacctt acgcggttga agcgagtgaa ttcgtaaaac cgggtaaagg
     1441 ccaggcattt gctcgcgtta aactgcgtcg tctgctgacc ggtactcgcg tagaaaaaac
     1501 cttcaaatct actgattccg ctgaaggcgc tgatgttgtc gatatgaacc tgacttacct
     1561 gtacaacgac ggtgagttct ggcacttcat gaacaacgaa actttcgagc agctgtctgc
     1621 tgatgcaaaa gcaattggcg acaacgctaa atggctgctg gatcaggcag agtgtatcgt
     1681 aactctgtgg aatggtcagc cgatctccgt tactccgccg aacttcgttg aactggaaat
     1741 cgttgatacc gatccgggcc tgaaaggtga taccgcaggt actggcggca aaccggctac
     1801 cctgtctact ggcgctgtgg ttaaagttcc gctgttcgta caaatcggcg aagtcatcaa
     1861 agtggatacc cgctctggtg aatacgtctc tcgcgtgaag taatgcggtt gtggtgcggc
     1921 ctgcaggctg caccatcact tattcaggtc agagatgatg aaacgcctta tcgttcttgt
     1981 tttgcttgcc agcacgctgc tcacgggctg taacaccgct tgcggtttcg gcgaagacat
     2041 caaacatctc ggcaactcca tctctcgcgc tgccagctaa tttttcttct cttccgaaaa
     2101 atcatcagat ttccatcatt tttggcgatg ttgtctatta ttaatttgct ataggcaaac
     2161 ataaataaca ttacctaaaa ggaagacgtt atggtgaaga agacaattgc agcgatcttt
     2221 tctgttctgg tgctttcaac agtattaact gcctgcaaca ccacgcgtgg cgttggtgaa
     2281 gacatttctg atggcggtaa cgcgatttct ggcgcagcaa cgaaagcgca gcaataagca
     2341 ataacggtac gacagcagtg tcgtgccgtt tgttttttct gcgatcgtca taaaggtaat
     2401 agttgaaatt cccctgccac ctggcaaaat atccgttcaa ccatcagctt tgcaggacga
     2461 cctgcaaacg cctcttttca ccggggacgg ccccaaattt ccggagcctg atatgtccta
     2521 gattatctta gttattgctg gtctgctgga agtggtatgg gccgttggcc tgaaatatac
     2581 ccacggcttt agccgtttga tgccgtgtgg acgggtattg gcgcagtcgg cgcggccatc
     2641 accggcattg tgctgctcgg tgagtccgct aacccgatgc gtctggcgag tctggcgtta
     2701 atcgtattgg ggattattgg tctgaaactc agcactcact aactaccggg ctgctgtacc
     2761 caaataaact tactgacatc aaacccttcc cgggtcgcga ctgccagcat ctcctgtttc
     2821 acttcgtcag aaatggttgg cgtgcgggag agtatccaca ggtagtcgcg gtccgggccg
     2881 caaaccagcg catggcggta ttcccgatcg agtgcaataa cgttataacc gccatagaaa
     2941 ggaccaaaga atgacacttt cagcgcagcg cgagttggtg cgccggtaaa gtacgctttc
     3001 ccttcactct gctgccacat tcctctgtca gggttatagc ctttattaat gacattcagg
     3061 ccgccgtcat cacgcaggct gtatgttgcg gtgacttttt ccagtccacg ttcaaaacgg
     3121 tgatcaaaac gggcaatctc ataccaggta ccgagataac gtttggcgtc gaaattattc
     3181 actacggtca cgccacgcgg cggcgtagga gaactacagg caacgaccag aaatgcagct
     3241 gtcgctgcgg caacgagagg gagcaggcgc ataaatgttt ccttactggt ttttttctaa
     3301 gtgtagatga cagcaaggaa aagcgcagaa aaggtccgaa aattcggacc cgatgaaatt
     3361 ttactgtaga gcgttgagaa tctgacaggc ggcggcgact ctctctggat tgggatagtt
     3421 tttgttagcc agcatcacga tacccagctc tttttctgga ataaacgcga catagctacc
     3481 aaatccgccg gtcgcccccg ttttatgtac ccatgatgcg cgtactgcag gagttggggg
     3541 cttaatcgct tttaccgggc gtgctgccag tgcaatttta ttgtcactgc cgttaatgat
     3601 gctgtcagga ttgaccggcc agtccagcat ttcccagccc aggccctgat acatatcgcc
     3661 ggtttgccag tagcgagatt gtgccagttg tatcccttgt tgaagcgttt tctcattgat
     3721 atcaaggggg ggtaaattgc tttgcaccca gcgggccata tcttcaatgg tcgacttcac
     3781 accataagct tcagcatcta acgcccctgg cgaaacatgc actgccttac cttcgcgata
     3841 tccccaggcg taattctttt tttctggggg aggtacatta atccacgtat ggttgagttt
     3901 gagtggctgg aagacacgag tttgcatcgc ctgctcaaaa ctcaaaccag acggcttcac
     3961 agccagtgcg ccgaacaaac cgatactgga gttggcatac agacgttgtg ttcctggagc
     4021 ccatgcaggc tgccagtttt gatagaagcg cagcaagtcg cttgaggatt tcacttcatc
     4081 cggcacctgc aatggcaggc cgccagcggt gtaggtcgca agatgtaata gtgtgatccc
     4141 attccactgt ttagcggtaa gttcaggcca gtattttgtt gcgggatcgc ttaacttgat
     4201 ttcccctcga gcaatagcgt cgccaccaag cacgcccgta aatgttttgc tgaccgaacc
     4261 taactcaaac aacgtttgct gtgtgacggg ctgctttttg gcgatgtccg catagcccca
     4321 ggtaaagtaa taaggtttac cctgataaat taccgccacc gccataccgg ggatcttttg
     4381 ttgctctata agcggggtaa ttgtgcgatg cacaatatcg ttgatttgtt gaggggcagc
     4441 aaatgtggag caagaggcgg taattaataa ggcgcagagc gtcgttttga acataaggtc
     4501 tggtttccat acaaaacggc ccgccatagg cgggccggat ttacattggc gatgcgttag
     4561 attgtaacga caccaatcag cgtgacaact gtcaggatag cagccagacc gtagaaaacc
     4621 catttgcccg caggcacgtg gattttcaga tcgtgcatcg cgtggtgcat acggtgtaaa
     4681 ccacaccaca gcggcagaac gatcatcagg aacaggaata cgcgaccaat gaagctctgc
     4741 gcgaacgcca gaacgcgctc gtagctcagc gcatcacccg gaaacagccc cagtggcagc
     4801 agaataccca ccagcaggat catcaccggc gcaataattg cgctccacat accaccggcc
     4861 ccgaagaggc cccagaatac cggttcgtca gaacgctttg gatttggatt aatcatctca
     4921 ggctccttac cagtacaggg caacaaacag gattacgatg gtggcaacca cagttaccgc
     4981 ccagagactt ttgataattg gctctggtcc cattttttcg tcttttacaa tgatattggc
     5041 cgctttcggt gccagttcaa accaggtttt ggtgtgcagc agagctgccg tcagagtgat
     5101 caggttaatg atcacgataa ccgggttttg taaaaagtcg acgaatcccg cccaggcttc
     5161 cgggccattt ttcagggcaa acagcccgaa aatcagttca atgctgaacc acacagccgg
     5221 aaccgccgtg ccttcgcgca gcatgtaaaa gcgataaaac ggcaattttt tccaccaggt
     5281 ggacgtcatt ggccgtacat acggtttacg tttagtcgtc atgttgcact ccttagcgtg
     5341 gtttcagggt cgcgataaga aagtctttcg aactttctac tttgccctgc tgaatggccg
     5401 cagccggatc gacgtgtttc gggcagactt cggagcaata gcccacgaaa gtacagctcc
     5461 atacaccgtt ctggctgttc aactgcgcca tacgctcctt cttaccgtgg tcgcggctat
     5521 cttcgttata acgatgcgcc agcgtaatgg cagccggacc gatgaactct gggttcaggc
     5581 caaactgcgg gcacgcggcg tagcacagac cacagttgat gcaaccggag aactggtgat
     5641 actttgccat ctgcgccggg gtctggatgt tagtaccctg atccgcggtg cgggagttgc
     5701 cgatgatgta cggtttgatc gcttccagac tttcgatgaa gtgggtcata tcgaccacca
     5761 gatcgcgttc aatcgggaag ttagctaacg cttcaacctt cataccgtcg gtgtaatcac
     5821 gcaggaaggt tttacatgcc agttttggca cgttgttaac catcatgccg caggaaccac
     5881 aaatcgccat acggcaggac cagcggtaac tcaggtccgg tgccaggttg tctttgatgt
     5941 agcccagcgc atccagtaat gaggtagttg cgtcataagg cacttcatag aatgcgctat
     6001 gcggtgcggt atcgacttcc gggttatagc gcaccacctc aattttcagg tttttcatct
     6061 cagccattcg ccttctcctt attattggct gcttccgcct tatcggctgc atccgcttcg
     6121 ccaccgtaaa cgcgtttagc tggcggcagc gtagtaatct tcacgtcgct gtactccagg
     6181 caagtcgtgc catcagcatc gcggaaggcg agggtgtgtt tgagaaagtt gacgtcgtca
     6241 cgctcggtgc aaccttcgtc cagacgctgg tgtgcgccgc gggactcttt acgtgccatt
     6301 gcggagtgcg ccatacattc agcaacgttc agaccgtggc tcagttcaat ggtgtagagc
     6361 aggtcggtgt tgaacacgct ggaggtgtcg gtgatgcgca cgcgcttgaa gcgttcctgc
     6421 agctctgcca gcttgtcgat ggttttctgc atcagttccg gcgtacggta gataccgcaa
     6481 ccttcttcca ttgccaggcc catttcgtcg cggatcttcg cccagttttc gccgccatcc
     6541 tggttaacca gatctttcag acgttgttca acgccagctg cctgcgcttc aattgccgct
     6601 tcgttgccat taccggcagt tgctgcacgc tctgtcgctt gttcaccggc cagacggccg
     6661 aagaccacca gttccgccag ggagttggag cccagacggt ttgcaccgtg cagaccaaca
     6721 gaggaacatt cacccacggc gaacagacct ttaatgcggg tttcacagtt ctgatcggtt
     6781 tcgataccgc ccatggtgta gtgtgcagtc ggacgtaccg gaatcggttc tttaaccgga
     6841 tcgacgccaa cgtacgcttt cgccagttcg cagatgaacg gcagacgttc atgcagtttt
     6901 ttctcgccga ggtgacgcag gtcgagataa accacatcac cacgcggcgt ggagatggtg
     6961 ttgcctttac gccattcgtg ccagaaggcc tgagaaactt tgtcgcgtgg acccagttcc
     7021 atatatttgt ttttcggctc gcccagcgga gtttccgggc ccatgccgta atcttgcaga
     7081 taacggtagc catttttgtt gaccagaata ccgccttcac cacggcagcc ttcggtcatc
     7141 aggataccgg aacctggcag accggttggg tgatactgaa cgaattccat gtcacgcagc
     7201 ggaacgccgt ggcttagcgc catacccata ccgtcaccgg taacgatgcc gccgttggtg
     7261 ttgtaacgat aaacgcgacc cgcaccgccg gtagccataa cgaccgcgtt agcacggatc
     7321 tgcaccaacg tgccttccat catgttcatt gctaccaggc cgcgaacatg accatcatca
     7381 accagaatat ccagcacgaa atgttcgtca aaacgctgga tctgcgggaa ttgcagagag
     7441 gtctggaaca gcgtgtgcag catatggaag ccggtcttat cggcggcgaa ccaggtgcgc
     7501 tcgattttca tgccgccgaa gcgacgtacg ttgacgctac catccgggcg acggctccat
     7561 gggcatcccc acagttccag ttgggtcatt tcggttgggc agtggtggac gaaataatcc
     7621 acgacatcct gctcacacaa ccagtcgcca cccgctactg tatcgtgaaa gtgatattcg
     7681 aagctgtcat gatcctgcgc gacagcggcg gagccccctt ctgcagcaac ggtatggcta
     7741 cgcatcgggt atacttttga gattagtgcg atttttgcat ttggatttgc ctgcgcggca
     7801 gcaattgcag cacgtaatcc cgcgccaccg gcgcctacaa tggcaagatc ggcttgaaag
     7861 gtttgcacga cattcctcca gattgttttt atcccacagc cccgtacttc agggtaagtg
     7921 cctgaaagtt acggtctgcg aacgctattc cactgctcct ttataggtac aacagtatag
     7981 tctgatggat aagtctgaaa tttgacgaga tcgatttttt tagtgcgcaa gagggctaaa
     8041 ttatcactga agatgattaa tttaattact aaaccatcag atcgtgcttt ttttagtcac
     8101 tgcccaccgc tgtttgattc ctgcgtaaaa tttgtctcgc cgtcgcgttg cgagtagact
     8161 tcgtgccctt gtcaaaaact ggagatttaa ctatgagcga aacggcatcc tggcagccga
     8221 gcgcatccat tcctaactta ttaaaacgcg cggcgattat ggcggagatc cgtcgtttct
     8281 ttgccgatcg tggagtgctg gaggtggaga cgccttgtat gagccaggcg acggtaaccg
     8341 atattcattt ggtcccgttt gagacacgtt tcgttggccc cgggcattcg caggggatga
     8401 atctctggtt aatgaccagc ccggaatacc atatgaaacg cctgctggtt gctggttgtg
     8461 ggccggtatt ccagctgtgc cgcagcttcc gtaatgaaga gatggggcgt tatcacaacc
     8521 ctgagttcac tatgctggag tggtatcgac cgcactatga tatgtaccgg ttgatgaacg
     8581 aggtggacga tctcttacaa caggtgctgg actgcccggc agcagaaagc ctttcttatc
     8641 aacaagcttt cttgcgttat ctggaaattg acccgctctc tgccgacaaa acgcaactgc
     8701 gggaagtcgc agcgaaactg gatttgagca atgttgctga taccgaagaa gaccgcgaca
     8761 cgttgctaca attgctgttt acctttggcg tagaggcaaa tattggtaaa gaaaaaccga
     8821 cctttgtgta ccactttcca gccagccagg catcactggc gcaaatcagt accgaagatc
     8881 atcgggtcgc tgaacgcttt gaggtttatt ataaaggtat tgagctggcg aatggtttcc
     8941 atgaattaac ggatgcccgt gagcagcaac aacgctttga acaagataac cgtaagcgcg
     9001 cggcgcgcgg tttgccgcag caccccattg accagaatct gattgaagcc ttgaaagtcg
     9061 gtatgcctga ctgctccggc gtggcattag gtgttgatcg tctggtgatg ttggcgctgg
     9121 gcgcggagac actggctgaa gtcatcgcct ttagcgttga ccgggcataa ttctgaaaaa
     9181 ttactgaatt aacagcgaag aatggcgtga taagcgccat tctgtagcat atgttttcat
     9241 ttttccgtta aaggccacaa tttaagactg gatatttgat atcatcaagg tatcaattct
     9301 gtattggttt ttacgctgcc tgctcaatgt tgcgcagtgc agttcagtgg gcacgtgtta
     9361 tacacgcgct gaaatgaagg atggtttcat gcctcacacg ataaaaaaga tgagtctgat
     9421 aggactcata ttgatgatct ttacttccgt atttggattt gccaatagcc catcggctta
     9481 ttacttaatg ggttatagtg cgattccctt ttatatattt tctgcattgt tattctttat
     9541 tccattcgcc ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat
     9601 ctattcctgg atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg
     9661 gttttcctct tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc
     9721 aacattcctc tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac
     9781 gcaggtggtt ggtctgctgg cagtagcatg gatgattctg gtcaccgtcg ttgcttcaaa
     9841 ggggattaat aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa
     9901 tttagtattg ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca
     9961 ggatattaat ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc
    10021 gtttgtggta tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga
    10081 taaaacggaa aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat
    10141 ttcaatcggt tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt
    10201 attaagtaat ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg
    10261 ggtgacgctg ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg
    10321 gtttgcgcgt attaccggac tttcgatgtt cctcgcttat accggtgcgt tctttacgct
    10381 ttgctattca ccgctgaaag ccatcatcca ggggacgccg aaagcgttgt ggccggaacc
    10441 gatgacgcgc ctgaatgcga tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt
    10501 ggttactgtc ttcattctgc tggtttcgtt tggtggcggt accgcatcgg cgttctttaa
    10561 caagctgacg ctgatggcga acgtgtctat gacgcttcct tacctgttcc tcgcgctggc
    10621 tttcccgttt tttaaagcac gtcaggatct cgacagacca tttgtgattt tcaaaacgcg
    10681 tatgtcggca atgattgcga cggtggttgt cgttctggtg gtgacatttg cgaacgtctt
    10741 caccattatt cagcctgtgg ttgaagccgg agactgggac agcacattgt ggatgattgg
    10801 cggccctgtc ttcttctcgc tgttagcgat ggcgatttac cagaactatt gcagtcgcat
    10861 ggcgaataaa cctgagttag ctctcgactg atcatatgaa atcctgcaac tcatcgtctc
    10921 tccggcgatg agttgtcagg aagtaatgcg tatggatgat actctccagg gccggtgata
    10981 actgaagatg atgttaagct gctaaatgag ccacgttgac atcatttggc agcttcatgc
    11041 attacaaact tcccgcctga cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt
    11101 taccgttgag gctttccaga cgcatctgga agggcgggaa tggcatatcg ataccgtggg
    11161 cgtggaagcc tgccagaatc agctggtgga tctcatggcg tagcggcata cggtgaccca
    11221 tctcagcggc gtaaatacgc agctcgaaaa tctgaatccc ctgttgcaga tccaccagga
    11281 agacttccgg tgccgggttg tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga
    11341 tttccgtcac ttcttcactg ttggcatcgg caggggccgg aatcgtcaac actacgcgcg
    11401 tgaccgagtc agagagcgac cagttgataa actgctcggt aataaacgcc ttgttcggca
    11461 cgattatctc tttacggtcc cagtcgctga tggtggtggc gcgggtgtta atttttgtca
    11521 cgctaccagt aagatcgcgg atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca
    11581 ggatgatcag gccagagata aagttggcga aaatttcctg caaaccaaaa ccgagaccaa
    11641 caccgagcgc ggcaaccagc cactgcaatt tcgaccactc aataccaatc attgagaagc
    11701 cgaccagccc gccaatcagc atcagcagat attttgtgat ggtggtgatg gcgtaacccg
    11761 tacccggcgt taaatccagg tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc
    11821 gcaccagctg cgtggtgatg ataaatacca gaatggcaat cagcaccgca ccgagggtaa
    11881 ttggctccag actttctacg ccctgtaccg tggaggtgac atcccacagc gaaatatttt
    11941 cgaggaagcc gaaagcggaa tggatttctg accacagcac aatgaccgaa cgcagggcga
    12001 tcaacatcaa aattgagcgc accagccgca aggattgcgc actgatggca tcgagatcga
    12061 cttcgctttc atcgacttca attgctcctt ccgggctact gtgatgatgc gcttcctctt
    12121 cgccacgcgc acgttgcgct aacatctctg cccggcgatg cttcgcccgg tcaaacgcca
    12181 gcctgcgacg ctggatcagc atccagcggc ggataacgtg ataaaccacc agtagcagga
    12241 accagatggc aaccgaggtt tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac
    12301 ccaccgccga cgccagaatg gcaaccagtg gtgcgccaat catcatgttc cacagcatat
    12361 ggttggtaat gttgtcgccg ctgccttctt tgttgagata caacggaata ccggcttttt
    12421 tcaggcttag ggtgaccacc gccagcgcac cgcaaatgag gataaagcaa agccgtccca
    12481 gcgaaccgga aaattcgcgg tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc
    12541 cgatgctcat caggtagtag cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat
    12601 gagcgataaa caagccgttc gggcgggcaa aggtggcgca aatcatcact acccacagca
    12661 gcggcacggt ggccgttaca ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct
    12721 cgcgcaagcc gtaacccagc gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg
    12781 accagaaaag agtgcgcaac gtcagccaga agtgatcctg agtcactttg ccgactttcg
    12841 ccgccgaacg ttcaagaaaa cgggtgaaat agcggcgcga gtaaatactg caaccgacca
    12901 gaatcaacgc gccaaacagc ggcaaaattg tctctttgct ggtcagcatc atcacactgg
    12961 ctttgcccaa ctgactgaag gtgtccagcg aaatgagacg acgcagatcc tgggcgattt
    13021 ccagcggcca ggcgatggtc atcgggcgca cgtcagaggt ccagaacaga tagcggtgcg
    13081 ttgcttcgtt cacctctttc agcgcatcct ccagttgccc gttggagact ttcagcttgg
    13141 tcagttccaa tagtagcgtg tcgccaccct gcaacaatga gttcagcaac tcacgctgag
    13201 tgcgcagttg tgcttccaga atacggtttt gctcggcggt cagcggctga ccgtcggcct
    13261 gatggatttg ccgtagcagc ggctgtttat taagtaaatc ctcataacgt aaccgttgca
    13321 cacgcaactg cgccatttcg gtatcaagct gttgtggttt tggcatttct ggtagccgtg
    13381 ccacctgcgc ccgcagcgct tcgccgagca ggttggacga tcccagccat tgcgactgtt
    13441 cacgcagcgt attcaacgcc tgccggacct gtaacgtctg gctggcagcc tgacgctgtt
    13501 gcgaggcaac gagctccatc cgctgcgcct gttgattcag cgctgccgat agttcgcggt
    13561 taattttgaa ttgcgcgacg atatctttcg gcaaatcggc actgttttct gccagtaatt
    13621 cggtactttc cagcgcccgc tccgcctcaa gctgacgttg gctattcaac tgattacgta
    13681 agccctgcaa atacgcatcc agttgctggc tctctttttc cgccagctct gagcgtaagc
    13741 gcgctaattc ctggcggtta ttggcagaca gctgcgccag ctccagttca tcaacgagcg
    13801 ccttaagacg tgcagagtca gactgcaacg cgaaattttg tgcctgattg agcggagtat
    13861 tgccggtaag cgttcccagg cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt
    13921 gctgttgcgg cagttgattc agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct
    13981 gggcctgacg gcttttatcc agcaactggc tgctgacctg gagaatttcc tgattcagcg
    14041 cgtcggtaga cattcccggc gacacgctgc gcggctcgtc acgcatgttg tttaattgtg
    14101 cgcgcagagt agcggagagt ttcggataat tatcgataac ttgctgatat tgtttgatgc
    14161 gctcaaggga accttttcgt tcctcaagcg catttaaggc agactggagc gcctctacga
    14221 cttccggctg tgcgggtttc gccgcttttg cctgctccag ttcctgagtg atttgtttgc
    14281 tatcgggggc cgtcgcggcg tacgcccccc aactgaggca ccaggccatc agaaaagtga
    14341 taatcaggcg cacgtcagcg tttcctttga tggattagac ctggtctttt ttatcgtcaa
    14401 ccaatggcct ggcgtcgtgt tctgcttcga tctcttcagc aggaagtggg gcgggttcag
    14461 cgtctggcgt aacaaaggtt tcggtagata ctgccagcgg ctggccaatt ttcgtgacag
    14521 acaggctttc cagttgctca accagattca ctttacccgg tgcaaacagg ttgataacgg
    14581 tggaaccgag tttaaagcga cccatttcct ggcctttcag cagtgccaca gaaccgtcgt
    14641 tttccccggc aggccaggtc cagcgcttga tgataccttc gcgcggcggc gtaatggtgc
    14701 ccgcccagac cgtctcaatg ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg
    14761 ggccaaattc ggtatcgaaa aggcaaatca cgcgttcgtt acgggcaaac agattcggca
    14821 cgttctgagc ggtgagatgg ttaacggaaa agagatcgcc cggcacgtag atcatctcac
    14881 gcagaatacc gttgcacggc atgtgtacgc gatggtagtc acgcggggag aggtaagtgg
    14941 tcacaaacgt accgttgcgg aacaggtccg ccatcagata gttgcctgcc agcagggctt
    15001 cgaggctgta gttgtggcct ttggcttgca ggattttatc ttcttcgatt ttacccaact
    15061 ggctgataac gccatcggca ggcatgacca gtacattcgg atcggtatcg attgggcgta
    15121 cttcgtcacg cagcggacgg acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg
    15181 gcttttgcgc ctctttcatg tcgaccttgt agtatttaac gaacagatcg ataaccagtt
    15241 ttgtcagcca tcctgcccgc ttgcttgcgc cccaacccgc caggcgagta agccatagtt
    15301 tcggcagaat gtactgtagc gaaagtttaa atgaatttaa caaggtagcc tccaggccat
    15361 tgttttgtcg ttcctgatcc ggccaacatg ccggatcctg aaaaaaaggg gacgattcta
    15421 acgacggtta gcttaattgt cagtcatccg tatcagaaaa gtttttacgc gtttttacct
    15481 gcgccatgct ttccagaata cggtgatagt tttcgaaacg ggtttccgcg attttccctt
    15541 cctcaaccgc ttcccggata gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt
    15601 tacacagacc taaatagtca tggaattcga caaagccctg agtgatttgt tccggctcca
    15661 ggtgccagag gccgaactca cgcacaccag gggaatcaat cacatcaccg ccgtgcggga
    15721 agtgatacag ccgagcggcg gtcgtggtgt gctgaccgag ccccgagttg tcagagacat
    15781 cgttggtcag gatctctttt tgcagcccca gcaacgcgtt cagcaggctg gatttaccga
    15841 cgccagactg cccggcaaaa atgctgatgc gcccggtcaa cgcctcttcc agcggtttca
    15901 gcccatcctg agtatggctg gaaaccatca atacgcgata accgatgttg cggtagatat
    15961 ccatctgctc gttgacgaac gccatgcctt cgtcgtccag caggtctatc ttgttgagca
    16021 cgataatcgg ctcaatctgc aaggtttcgc aggccaccag gtaacggtcg ataatattga
    16081 gcgacagctc cggcaaaatg gcggagacaa tgacaatctg gtcgatgttg gcggctatgg
    16141 gtttcacgcc gtcgtagaaa tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt
    16201 ccacgatccc tttgacattt acgccttccg ccgccggttt acccggacgc cagactacgc
    16261 ggtcgccggt taccagcgaa cggatggtac ggcgaatatt gcagcggtga acgtcgccat
    16321 cggcggattc cacatcagcg tgcataccaa agcggctgat gacgatacct tcatcaggct
    16381 cgccaaacag attgtcgtcg tagtcgggct tctccttaga cgttttaaga cgacgctggt
    16441 gattggcgtt cacgcggcgc tgctggcctt tggagagttt atttttactc aatcgtactg
    16501 actcctggtc gcccctgatg ggcaaaacat ctatgataca cgcaattgtg gatcaatata
    16561 gtcactgtga atgggtggaa aatagcatga gtgccaatga aaacaacctg atttggatcg
    16621 atcttgagat gaccggtctg gatcccgagc gcgatcgcat tattgagatt gccacgctgg
    16681 tgaccgatgc caatctgaat attctggcag aagggccgac cattgcagta catcagtccg
    16741 atgaacagct ggcgctgatg gatgactgga acgtgcgcac ccataccgcc agcgggctgg
    16801 tagagcgcgt gaaagcgagc acgatgggcg atcgggaagc tgaactggca acgctcgaat
    16861 tcttaaaaca gtgggtgcct gcgggaaaat cgccgatttg cggtaacagc atcggacagg
    16921 accgtcgttt cctgtttaaa tacatgccgg agctggaagt ctacttccac taccgttatc
    16981 tcgatgtcag caccctgaaa gagctggcgc gccgctggaa gccggaaatt ctggatggtt
    17041 ttaccaagca ggggacgcat caggcgatgg atgatatccg tgaatcggtg gcggagctgg
    17101 cttactaccg cgagcatttt atcaagctgt aaaatttttc ctggtcacgt aagcgccgga
    17161 atccacttgc ggcggcgtga ttttgacgct aaatcgaacg tcttgctgat aatttcagca
    17221 cttgagataa aaacgcaaaa aaaacttttt tggggggttg cagagggaaa gatttctcgt
    17281 ataatgcgcc tcccgtaacg acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt
    17341 a
//
LOCUS       DADMEQ010000078        17302 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.079, whole genome shotgun sequence.
ACCESSION   DADMEQ010000078 DADMEQ010000000
VERSION     DADMEQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.079"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..600)
                     /locus_tag="JNZ05_002644"
     CDS             complement(<1..600)
                     /locus_tag="JNZ05_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8565828.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHKVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIVNYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPRTKWTANKVARYLYISVSTLHRRLA"
     gene            complement(610..1161)
                     /gene="ybcL"
                     /locus_tag="JNZ05_002645"
     CDS             complement(610..1161)
                     /gene="ybcL"
                     /locus_tag="JNZ05_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HAY8565829.1"
                     /translation="MKKLVVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(1330..1662)
                     /gene="emrE"
                     /locus_tag="JNZ05_002646"
     CDS             complement(1330..1662)
                     /gene="emrE"
                     /locus_tag="JNZ05_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HAY8565830.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2007..2321)
                     /gene="fliE"
                     /locus_tag="JNZ05_002647"
     CDS             complement(2007..2321)
                     /gene="fliE"
                     /locus_tag="JNZ05_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAY8565831.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAAIDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2535..4193
                     /gene="fliF"
                     /locus_tag="JNZ05_002648"
                     /pseudo
     CDS             2535..4193
                     /gene="fliF"
                     /locus_tag="JNZ05_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
     gene            4186..5181
                     /gene="fliG"
                     /locus_tag="JNZ05_002649"
     CDS             4186..5181
                     /gene="fliG"
                     /locus_tag="JNZ05_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HAY8565832.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVNDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5174..5860
                     /gene="fliH"
                     /locus_tag="JNZ05_002650"
     CDS             5174..5860
                     /gene="fliH"
                     /locus_tag="JNZ05_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HAY8565833.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQVHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAALGVV"
     gene            5860..7161
                     /locus_tag="JNZ05_002651"
     CDS             5860..7161
                     /locus_tag="JNZ05_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HAY8565834.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAAYATRIAEDFRDRGQHVLLIMDSLTRYAMAQREIALAIGEPPATKGYPPS
                     VFAKLPALVERAGNGISGGGSITAFYTVLTEGDDQQDPIADSARAILDGHIVLSRRLA
                     EAGHYPAIDIEASISRAMTALISEQHYARVRTFKQLLSSFQRNRDLVSVGAYAKGSDP
                     MLDKAIALWPQLEGYLQQGIFERADWEASLQGLERIFPTVS"
     gene            7180..7623
                     /gene="fliJ"
                     /locus_tag="JNZ05_002652"
     CDS             7180..7623
                     /gene="fliJ"
                     /locus_tag="JNZ05_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HAY8565835.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7620..8747
                     /gene="fliK"
                     /locus_tag="JNZ05_002653"
     CDS             7620..8747
                     /gene="fliK"
                     /locus_tag="JNZ05_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HAY8565836.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRMHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8852..9316
                     /gene="fliL"
                     /locus_tag="JNZ05_002654"
     CDS             8852..9316
                     /gene="fliL"
                     /locus_tag="JNZ05_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HAY8565837.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            9321..10325
                     /gene="fliM"
                     /locus_tag="JNZ05_002655"
     CDS             9321..10325
                     /gene="fliM"
                     /locus_tag="JNZ05_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HAY8565838.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10322..10735
                     /gene="fliN"
                     /locus_tag="JNZ05_002656"
     CDS             10322..10735
                     /gene="fliN"
                     /locus_tag="JNZ05_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HAY8565839.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILNN
                     GYLIAQGEVVVVADKYDVRITDIITPSERMRRLSR"
     gene            10738..11103
                     /gene="fliO"
                     /locus_tag="JNZ05_002657"
     CDS             10738..11103
                     /gene="fliO"
                     /locus_tag="JNZ05_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HAY8565840.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            11103..11840
                     /gene="fliP"
                     /locus_tag="JNZ05_002658"
     CDS             11103..11840
                     /gene="fliP"
                     /locus_tag="JNZ05_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HAY8565841.1"
                     /translation="MRRLFSVAPVLLWLIMPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11850..12119
                     /gene="fliQ"
                     /locus_tag="JNZ05_002659"
     CDS             11850..12119
                     /gene="fliQ"
                     /locus_tag="JNZ05_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HAY8565842.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12128..12913
                     /gene="fliR"
                     /locus_tag="JNZ05_002660"
     CDS             12128..12913
                     /gene="fliR"
                     /locus_tag="JNZ05_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HAY8565843.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFIDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            13198..13822
                     /gene="rcsA"
                     /locus_tag="JNZ05_002661"
                     /pseudo
     CDS             13198..13822
                     /gene="rcsA"
                     /locus_tag="JNZ05_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
     gene            complement(13866..14108)
                     /gene="dsrB"
                     /locus_tag="JNZ05_002662"
     CDS             complement(13866..14108)
                     /gene="dsrB"
                     /locus_tag="JNZ05_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HAY8565844.1"
                     /translation="MPEKRNCSTLAQSHRRKTMKVNDRVTVKTDGGPRRPGVVLAVEE
                     FSEGTMYLVALEDYPLGIWFFNEAGHQDGIFVEKAE"
     gene            14217..14444
                     /gene="yodD"
                     /locus_tag="JNZ05_002663"
     CDS             14217..14444
                     /gene="yodD"
                     /locus_tag="JNZ05_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HAY8565845.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14742..15557
                     /locus_tag="JNZ05_002664"
                     /pseudo
     CDS             14742..15557
                     /locus_tag="JNZ05_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(15554..17248)
                     /gene="yedQ"
                     /locus_tag="JNZ05_002665"
     CDS             complement(15554..17248)
                     /gene="yedQ"
                     /locus_tag="JNZ05_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HAY8565846.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNFLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFMRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLIFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLARVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
BASE COUNT         4363 a         4393 c         4446 g         4100 t
ORIGIN      
        1 tgccagacgg cgatgcaatg tggaaacaga aatgtagaga tatctcgcta ccttgtttgc
       61 tgtccatttt gttctaggtt cggatagcat taggttataa caacgactta ttaatgattg
      121 tttactatct gataaaagta aacaattaac atgatgtgct cctaatgaaa gtagaacgcc
      181 cattgctaag tgttcctgaa tttgcgttga gaagcctcgg gaaatagatg tttttagttg
      241 ctcccaacaa tatattaact cggggttctg aggtaagcag aaacctgtcg tgttacgaat
      301 ttgatcagtt atcgcataaa atttttgaaa cttttcaatt agatcaatgg gataataaag
      361 catttctgca agataaagac ctgcttctgg ataattcaca atataaaatt catatccaca
      421 gggaaataat attatctgat tattatcaac agttagacta tgcgtctccc aattgataac
      481 tttttttccc tgacggatac gacataaagc tggcataaga ggcttaacct tgtgaatctc
      541 atgatgttta tgcatccgta tttcttcaat ctttaggtta gacttatctt ttgccagcat
      601 actcttaccc tactttatct cataaactgg tgttatctca gcggttgcaa ttttattggc
      661 attaagcata taaccaacta acgctccgct ggaattagaa tccacaggaa tcttatcagt
      721 ttttagagcc catactttaa actggtaatg atggggttta tctcctttgg gaggacatgc
      781 tccaccaaat cctgcataac caaaatcatt tcggccttga acagcaccag tcggaagttt
      841 tgtaccatca cgtcttcctg catcagtggg caaatatgtt acagttgctg gaatattagc
      901 aacagtccag tgccaccatc cactgcctgt aggagcatct ggatcataca cagttacggc
      961 aaagcttttg gtgccttcag gagcaccaga ccaggttaat gagggcgatg tattaccacc
     1021 ttcacaccca aatccagaaa atacatgaga cgttgtaagt tgctctcctg tttttatttc
     1081 attactagtg acctgaaatg ctgcggcctg cgcagaaaat gttatgaatg ccaatacact
     1141 tgaaacgaca agttttttca taaaaacctc attgttgtga cctatcgtta ttttatttga
     1201 tgtgccttga tctcattatg cataaaggca caatgatcgc gcaaatgcaa tcataattat
     1261 aaaggtgccc cgaacattag ccataatatg cacaaatcag attcctgtta tctcaaggag
     1321 aattattttt taatgcggtg cacttcgtga caacaagtta atgactaaca caccggcaca
     1381 aatcaacatc atgcctatga tagctggcag gtccagtcgt tgaccaaaaa atccccagga
     1441 aagcaaacta atcagaacga taccgactcc tgaccagata gcataagcaa tccctgtagg
     1501 tatataagcc agcgtctgag ctaataacca gaatgatgca caatagcaaa taattgtgcc
     1561 aacagagggc cataaccgtg taaacccctc tgaaaacttc attaaggttg taccaatgac
     1621 ctctgcaagt attgcgccac caagataaat ataagggttc atagcatatt ctttcctgtt
     1681 catactggag agaattgtac tacagtttga acacaactca ccgttttcat cctagtgtat
     1741 gtgtatccaa ggggctctgg cgtggcaaga gataaaggca ggaacaataa tgatcagaag
     1801 gaaccccagg ctaacgaatg agttcactct tcctgggagc aaagcgaaga caaaacatgt
     1861 tatcgcgttt acggatcgtg gatggcagaa aacaaccagg atcaggtatc cattttaaac
     1921 cagaaattga gtgagtttgc cccatccatg ccccacgctg tgggatcgga tataattaaa
     1981 caagcatagt caacaggtta cacaacctac acctgcatag acatcacttc ctgatacgcc
     2041 gccaccagct tattacgcac ctgaatcccc atttgcatag aaactgaggc tttttgcata
     2101 tcggtcatca catcgtttag cgccacgccg ggttcaccga gggtgaattt ttctgcctgc
     2161 gtgcgggcga ctgtttgtgt atcgcttatg cgatcgatcg cggcgtgcag ctgcccggca
     2221 aaactaatgg tcggttgcgg cagtgattcc tgcgcacgcg cactcatcgc cgtagcctgt
     2281 aactggctga taaccccttc aatcccctgt atcgctgaca ttttcgtctc ccggataatt
     2341 tctggtaaca aagcctacca gtaagtcaat aagacaaagg cgctaaatag caacaaaaaa
     2401 acgggtttat tggcggatag aaaaaacgaa agcacaaata atgggagcgt caatttttcg
     2461 agtttgctga cccgagagtg agtcttgttc cactttgcca ataacgccgt ccataatcag
     2521 ccacgaggtg cgcgatgaat gcgactgcag cccagacaaa atctcttgag tggcttaatc
     2581 gcctgcgtgc gaatccgaaa attccattga ttgttaccgg ttccgcggca gtggcggtca
     2641 tggtcgcact gatcctgtgg gcgaaagccc ccgactaccg cacattattc agcaatcttt
     2701 ccgatcagga tggtggcgca attgtcagcc aactgacgca aatgaatatt ccttaccgct
     2761 tcagcgaagc cagcggcgct attgaggttc cggcagataa agttcacgaa ctgcgcctgc
     2821 gcctggcgca acaaggtttg cccaaaggcg gcgcggtcgg tttcgaactg ctcgatcagg
     2881 aaaagtttgg tatcagccag ttcagcgaac aggtgaatta tcagcgggcg ctggaaggcg
     2941 agctttctcg taccatcgaa actatcggcc cggtaaaagg ggcgcgcgta catctggcaa
     3001 tgccgaaacc gtctttattc gtccgtgaac aaaaatcccc ttctgcatcg gtgacggtaa
     3061 atctgttacc cggccgcgca ctcgatgaag ggcaaattag cgctattgta catctggttt
     3121 ccagcgcggt tgctggtctg ccgcctggaa acgtcacgct ggtggatcag ggcgggcatc
     3181 tgttaaccca gtccaatacc agcgggcgcg atcttaatga cgctcagttg aaatatgcca
     3241 gcgatgtcga aggccgtatt cagcggcgta ttgaagcgat cctgtcgcct attgttggta
     3301 acggtaatat tcacgcctag gtcacggcgc aactggactt cgccagtaaa gaacaaacgg
     3361 aagaacagta tcgccctaac ggtgatgaat ctcatgcggc gcttcgttca cgccagctta
     3421 atgagagcga gcaaagcggt tccggttatc cgggcggcgt accgggggcg ttgtcgaatc
     3481 aaccggcacc tgcgaataac gcgccaatca gcacgcctcc gacaaatcaa aataaccgcc
     3541 agcagcaggc gagcaccacc agcaatagtg ggccgcgtag cacacagcgg aatgaaacca
     3601 gtaactacga agtcgatcgc accattcgtc ataccaaaat gaacgtgggc gatgtgcaac
     3661 gtctgtcagt cgcggtagtg gtgaattaca aaaccttgcc agacggtaaa ccattgcctc
     3721 tcagcaacga acagatgaag caaattgaag ctctgacccg cgaggcgatg ggcttttctg
     3781 aaaaacgcgg tgattcgctc aatgttgtta actcgccgtt caatagcagt gacgaaagcg
     3841 gcggagcact gccgttttgg caacagcaag tgtttatcga tcagttgctg gctgccggtc
     3901 gctggttgct ggtactgctg gtggcgtggt tgctgtggcg gaaagcggta cgcccgcagc
     3961 taacacgtcg cgccgaggcg gtgaagactg tgcagcaaca ggcgcaggcc cgcgaggaag
     4021 tggaagatgc ggtggaagtc cgtctgagca aagacgaaca actccaacaa cggcgcgcta
     4081 accaacgact gggggcagaa gtcatgagcc agcgtatccg tgaaatgtct gataacgatc
     4141 cgcgcgtggt ggcgctggtc attcgccagt ggataaataa cgatcatgag taacctgaca
     4201 ggcaccgata aaagcgtcat cctgctgatg accattggcg aagaccgggc ggcagaggtg
     4261 ttcaagcacc tctcccagcg cgaagtgcaa accctgagcg ctgcaatggc gaacgtcacg
     4321 cagatctcca acaagcagct aaccgatgtg ctggcggagt ttgagcaaga agctgaacag
     4381 tttgctgcac tgaatatcaa cgccaacgat tatctgcgtt cggtattggt caaagctctg
     4441 ggtgaagaac gtgccgccag cctgctggaa gatattctcg aaacccgcga taccgccagc
     4501 ggtattgaaa cgctcaactt tatggagccg cagagcgccg ccgatctgat tcgcgatgag
     4561 catccgcaaa ttatcgccac cattctggtg catctgaagc gcgcccaggc cgccgatatt
     4621 ctggcgttgt tcgatgaacg tctgcgccac gacgtgatgt tgcgtatcgc cacctttggc
     4681 ggcgtgcagc cagccgcgct ggccgaactg accgaagtac tgaatggctt gctcgacggt
     4741 cagaatctca agcgcagcaa aatgggcggc gtgagaacgg cagccgaaat tatcaacctg
     4801 atgaaaacgc agcaggaaga agccgttatt accgccgtgc gtgaattcga cggcgagctg
     4861 gcgcagaaaa tcatcgacga gatgttcctg ttcgagaatc tggtggatgt caatgatcgc
     4921 agcattcagc gtctgttgca ggaagtggat tccgaatcgc tgctgatcgc gctgaaaggg
     4981 gccgagcagc cactgcgcga gaaattcctg cgcaatatgt cgcagcgtgc cgccgatatt
     5041 ctgcgcgacg atctcgccaa ccgtggtccg gtgcgtctgt cgcaggtcga aaacgaacag
     5101 aaagcgattc tgctgattgt gcgccgtctt gccgaaactg gcgagatggt gattggcagc
     5161 ggcgaggata cctatgtctg ataatctgcc gtggaaaacc tggacgccgg acgatctcgc
     5221 gccaccaccg gcagagtttg tgccaatggt cgagtcggaa gaaaccatta ttgaagaggc
     5281 cgaacccagc cttgagcagc aactggcgca actgcaaatg caggtccacg agcagggtta
     5341 tcaggcgggg attgccgaag gtcgccagca aggtcacgaa cagggctatc aggaaggact
     5401 ggcccagggg ctggaacaag gtctggcaga ggcgaaggct caacaagcgc caattcatgc
     5461 ccggatgcag caactggtca gcgaatttca aactaccctt gatgcgcttg atagtgtgat
     5521 agcttcgcgc ctgatgcaga tggcgctgga ggcagcacgt caggtgatcg gtcagacacc
     5581 aacggtggat aactcggcac tgatcaaaca gatccaacag ttgttgcagc aagaaccgtt
     5641 attcagcggg aaaccacagc tgcgcgtaca cccggatgat ctgcaacgtg tggatgatat
     5701 gctcggcgca actttaagtt tgcatggctg gcgcttgcgg ggcgatccca ccctccaccc
     5761 tggcggatgt aaagtctccg ccgatgaagg cgatctcgac gccagtgtcg ccactcgctg
     5821 gcaagaactc tgccgtctgg cagcactagg agtggtgtaa tgaccacgcg cctgactcga
     5881 tggctaacca cgctggataa ctttgaagcc aaaatggcgc agttgcctgc ggtacgtcgc
     5941 tacgggcgat taacccgcgc taccgggctg gtgctggaag ccaccggatt acaattgccg
     6001 ctcggcgcaa cctgtgtcat tgagcgccag aacggcagcg aaacgcacga agtagaaagc
     6061 gaagtcgttg gctttaacgg tcaacggctg tttttaatgc cgctggagga agtggaaggt
     6121 gtcctgcccg gcgcgcgtgt atatgccaaa aacatttcgg cagaagggct gcaaagcggc
     6181 aagcagttgc cgctcggtcc ggcgttatta ggtcgtgttc tggacggtag cggtaaaccg
     6241 ctcgatggcc tgcccttccc cgatacgacg gaaaccggtg cgctgattac cccgccattt
     6301 aacccgttgc aacgtacacc gattgaacat gtgctggaca ccggcgtgcg cccaatcaat
     6361 gccctgctta ccgtcggacg cgggcagcgt atggggctgt ttgccgggtc gggcgttggt
     6421 aaaagtgtgc tgctggggat gatggcacgt tacacccgcg ccgatgtcat tgtcgtgggt
     6481 ttgattggtg aacgtgggcg cgaagtaaaa gattttattg agaatatcct cggtgccgcc
     6541 tatgccacgc gcattgccga agattttcgc gatcgtggtc agcatgtgtt gctgattatg
     6601 gactccctca cccgctacgc gatggcccag cgtgaaattg cgctggcgat tggcgaacca
     6661 cctgccacta aaggttatcc accgtcggtg tttgccaaat taccggcact ggtcgagcgt
     6721 gccggtaatg gcattagcgg cggcggctcg attaccgcgt tttataccgt gctcaccgaa
     6781 ggcgatgacc agcaggaccc cattgctgac tccgcgcggg caatcctcga cggtcacatt
     6841 gtgctgtctc gccgactggc ggaagccggg cactatccgg ctatcgatat tgaagcgtcg
     6901 atcagtcgcg caatgacggc gttgatcagt gagcaacatt acgcgcgagt gcgcaccttc
     6961 aaacagctgt tgtcgagttt tcagcgtaac cgcgatctgg ttagcgtcgg cgcgtatgcc
     7021 aaaggcagcg atccgatgct cgataaagcc atcgccctgt ggccgcagct ggagggctat
     7081 ttgcaacaag gcatttttga acgcgcggac tgggaagcgt ctctccaggg gctggagcgt
     7141 attttcccga cagtgtcata acccaggaga taacggcaga tggcggaaca tggtgcgctg
     7201 gcgaccctga aagatctggc agaaaaagag gtagaggatg ccgctcgcct gctgggtgaa
     7261 atgcgtcgcg gatgtcagca ggcggaagaa cagctcaaaa tgctgatcga ttatcagaat
     7321 gaatatcgca ataacctcaa tagcgatatg agtgccggga tgaccagcaa ccgctggatc
     7381 aactatcagc agtttatcca gacgctggaa aaagccatta ctcagcatcg ccagcaactt
     7441 aatcagtgga cgcagaaagt tgacattgcc ctgaacagct ggcgagaaaa gaaacaacgt
     7501 ttgcaggcct ggcagacact gcaggaacgg caatccacgg cggcactgct tgcagaaaac
     7561 cgcctcgatc agaaaaagat ggatgagttc gcccagcgcg ccgccatgag gaaacctgaa
     7621 tgattcgctt agcgcccttg attaccgccg acgttgacac caccacattg cctggcggca
     7681 aagccagcga tgctgcacaa gattttctca cgttgttgag cgaagcatta gcaggcgaga
     7741 caacaactga caaagcggcc ccccagttgc tggtggcaac agataagccc acgacaaaag
     7801 gcgagccgct ggtcagcgat attcttgccg atgcgcaaca agcggattta ctgatccctg
     7861 tggatgaaac acttcctgtc atcaacgacg aacaatccac atcaacaccg ttaaccaccg
     7921 ctcagacaat gacgttggct gcggtggctg acaaaaatac gacaaaagac gaaaaagcgg
     7981 atgatcttaa tgaagacgtc accgcaagtc tgagcgccct ttttgcgatg ttgccgggtt
     8041 ttgacaatac gcccaaagtg actgatgcac cgtcaaccgt gttaccggca gagaaaccaa
     8101 cgctcttcac aaaactgact tctgcgcaac tcactacagc acagcctgat gacgcccccg
     8161 gcacaccagc tcagccatta acaccgctgg tagcagaagc ccagagtaaa gcggaagtca
     8221 tcagcacgcc ttcgccggtg accgctgacg ccagcccgct aatcactcca caccagacac
     8281 agccactgcc caccgtcgcc gcgcctgttt tgagtgcacc gctgggttct cacgaatggc
     8341 aacaatcatt gagccagcat atttcgctgt tcacccgcca ggggcaacaa agtgcagagt
     8401 tgcgtatgca cccacaggat ttaggtgaag tgcaaatttc cctcaaagtg gatgataacc
     8461 aggcgcagat ccagatgatt tcaccgcatc agcacgtacg cgccgccctg gaagcagcgc
     8521 tgccggtact gcgtacgcag ctggccgaaa gtggcattca gttagggcaa agcaacatta
     8581 gtggcgaaag ctttagtggt cagcagcagg ccgcttccca gcaacagcaa agccaacgca
     8641 cagcaaacca tgaacctctg gcgggggaag acgacgatac gcttccggtt cccgtctctt
     8701 tacaagggcg tgtaacaggt aacagcggcg ttgatatttt cgcctaacgt cagaggtagc
     8761 accgtaatcc gcgtcttttc cccgctttgt tgcgctcaag acgcaggata attagccgat
     8821 aagcagtagc gacacaggaa gaccgcaaca catgactgat tacgcgataa gcaagaaaag
     8881 caagcgatcg ctttggatcc cgattctggt attcattacc ctcgcggcct gtgccagcgc
     8941 aggttacagc tactggcatt cgcatcaggt tgccgctgac gacaaagcgc agcaacgcgt
     9001 cgtgccctcc ccggtcttct atgcgctgga tacctttacg gtcaatttgg gcgatgcgga
     9061 tcgcgtgctt tatatcggca taaccctgcg actgaaagat gaagcaaccc gctcgcggct
     9121 gagtgagtat ttgccggaag tccgtagtcg cttgctgtta ctgttttcac gtcaggatgc
     9181 tgccgtactg gcgacagaag aaggcaagaa aaacctgatt gccgagatta aaaccacact
     9241 ttccaccccg cttgttgccg ggcaaccgaa acaggatgtc accgacgtgc tgtataccgc
     9301 ttttattctg cgataacgac atgggcgata gtattctttc tcaagctgaa attgatgcgc
     9361 tgttgaatgg tgacagcgaa gtcaaagacg aaccgacagc cagtgttagc ggcgaaagtg
     9421 acattcgtcc gtacgatccg aatacccaac gacgggttgt gcgcgaacgt ttgcaggcgc
     9481 tggaaatcat taatgagcgc tttgcccgcc attttcgtat ggggctgttc aacctgctgc
     9541 gccgtagccc ggacattacc gtcggggcca tccgcattca gccataccat gaatttgccc
     9601 gcaacctgcc ggtgccgacc aacctgaacc taatccacct gaaaccgcta cgcggcacag
     9661 ggctggtagt gttctcgccg agtctggtgt ttatcgccgt ggataacctg tttggcggcg
     9721 atggacgctt cccgaccaaa gtggaaggtc gcgagtttac ccatactgaa cagcgcgtca
     9781 tcaaccgtat gttgaagctg gcgctggaag gctatagcga cgcctggaaa gcgattaatc
     9841 cgctggaagt tgagtacgtg cgttcggaaa tgcaggtgga atttaccaat atcaccacct
     9901 cgccgaacga cattgtggtt aacacgccgt tccatgtgga gattggcaac ctgaccggcg
     9961 aatttaatat ctgcctgcca ttcagcatga tcgagccgct acgggaattg ttggttaacc
    10021 cgccgctgga aaactcgcgt aatgaagatc agaactggcg cgataacctg gtgcgccagg
    10081 tgcagcattc acagctggaa ctggtggcca actttgccga tatctcgcta cgcctgtcgc
    10141 agattttaaa actgaaaccc ggcgacgtcc tgccgataga aaaacccgat cgcatcatcg
    10201 cccatgttga cggcgtcccg gttctgacca gtcagtacgg cactctcaac ggtcagtatg
    10261 cgttacggat agaacatttg attaacccga ttttaaattc tctgaacgag gaacagccca
    10321 aatgagtgac atgaataatc cggccgatga caacaacggc gcaatggacg atctgtgggc
    10381 tgaagcgttg agcgagcaaa aatcaaccag cgaaaaaagc gctgccgacg cggtgttcca
    10441 gcaatttggc ggtggtgatg tcagcggaac gttgcaggat atcgacctga ttatggatat
    10501 tccggtcaag ctgaccgtcg agctgggccg tacgcggatg accatcaaag agctgttgcg
    10561 tctgacgcaa gggtcagtcg tggcgctgga cggtctggcg ggcgaaccgc tggatattct
    10621 gaacaacggt tatttaatcg cccagggcga agtggtggtt gttgccgata aatatgacgt
    10681 gcggatcacc gatatcatta ccccgtccga gcggatgcgc cgcctgagcc gttagtgatg
    10741 aataaccatg ctactgtgca atcttccgcg ccggtttctg ctgagccact gctgcaggtg
    10801 agcggcgcac tcatcgccat tattgccctg atcctcgctg ctgcctggct ggtaaaacgg
    10861 ctgggatttg cccctaaacg cactggcgtt aacggtctga aaattagcgc cagtgcttcg
    10921 ctgggcgcgc gtgaacgggt cgtggtggtc gatgtggaag atgcacggct ggtgctcggc
    10981 gttaccgcag ggcaaatcaa tctgctgcat aaacttcccc cttcagcacc aacggaagag
    11041 ataccgcaga ccgattttca gtcggtcatg aaaaatttgc ttaagcgtgg cgggagatcc
    11101 tgatgcgtcg tttgttttct gtcgcaccgg tccttctctg gctgattatg cccctcgcct
    11161 tcgcgcaact gccgggtatc accagccagc cgctacctgg cggtggacaa agctggtcgc
    11221 tcccggtgca gacgctggtg ttcatcacct cgttgacgtt cattccagcg attttactga
    11281 tgatgaccag tttcacccgc atcattattg tttttggttt attgcgtaac gcgctgggaa
    11341 cgccctccgc gccacctaac caggtattgc tggggctggc actgtttttg acctttttta
    11401 ttatgtcgcc agtgatcgac aaaatttatg tcgatgcgta ccagccattc agcgaagaga
    11461 aaatctcaat gcaggaggcg ctggaaaaag gggcgcagcc gctgcgtgag tttatgctgc
    11521 gtcagacccg cgaggcagat ttagggttgt ttgccagact ggcgaatacc ggcccgttgc
    11581 aggggcctga agctgtaccg atgcgcattt tgctcccggc ctacgtgacc agcgagttga
    11641 aaaccgcatt tcagataggc ttcacgattt tcatcccttt tttgattatc gacctggtga
    11701 ttgccagcgt gttgatggca ttggggatga tgatggttcc ccccgccacc attgctctgc
    11761 cctttaaact gatgctgttt gtactggtgg atggctggca attgctggtc ggttcgctgg
    11821 cgcagagctt ttacagctag agaggcaaaa tgacacctga atcggtcatg atgatgggaa
    11881 ctgaagcgat gaaagtcgcg ctggcactgg cagccccgct gttgctggta gcgctggtca
    11941 cgggccttat catcagtatt ttgcaggcag ccacgcagat taacgaaatg acgctgtcgt
    12001 ttattccgaa aatcatcgcc gtatttatcg ccatcattat tgccggaccg tggatgctca
    12061 atctgttgct ggattacgtc cgcaccttgt tcactaacct gccgtatatc atcgggtagc
    12121 ccgtacaatg atgcaggaga caagcgatca atggctttcc tggttaagcc tgtacttctg
    12181 gccgttactg cgcgtgctgg cgctcatttc taccgcgcca attctgagcg aacgcagcgt
    12241 gccgaaacgg gtaaaactgg gtctggcaat gatgatcacg ttcgccattg ccccatcatt
    12301 acctgccaac gatgttcctg ttttttcgtt ctttgctctg tggctggccg tgcagcagat
    12361 cctgatcggc attgcgcttg gttttaccat gcaatttgcc tttgccgctg tgcgaaccgc
    12421 tggcgaaatt atcggtctgc aaatggggct gtcatttgcg acatttatcg atcctggcag
    12481 ccatctcaat atgcccgttt tagcgcgcat catggatatg ctggcattac tgctgttcct
    12541 gacatttaac ggtcatttat ggttgatttc actgctggtc gatacctttc acaccctgcc
    12601 gattggtagc gaaccgttga acagcaatgc gtttctggca cccaccaaag cagggagttt
    12661 gattttcctt aacgggctga tgctggcgtt accgctcatt actctgttgc tgaccctgaa
    12721 tctggcatta ggtttactga atcgtatggc cccgcaatta tccatttttg ttattggatt
    12781 tccattaact ctgactgtcg gcatctcttt aatggcggca ttaatgccgt taattgcgcc
    12841 tttttgcgaa catttattca gtgaaatttt taatttgctg gctgatatta ttagtgaatt
    12901 accattaata taattccgta acgtttatca tgctacccta aggattatcc gaaaaataat
    12961 atctgcgaac atcttccagg atactcctgt agcgaaatat ttgttttaag ctcactcaca
    13021 tatcgcaaca tttactttaa gacaattcca ggcaaattat acaacacttt acgggatagt
    13081 aagtccgcct gaaaaattgc gagagtggcg cattaggtga cccatgttgt tccgtttagt
    13141 catgatgaaa tattcaggta aggggaatta tcgttacgca ttgagtgagg gtatgccatg
    13201 tcaacgatta ttatggattt atgtagttac acccgactag gtttaaccgg gtatctgttg
    13261 agtagagggg ttaaaaaaag agaaatcaac gacattgaaa ccgttgatga ccttgccata
    13321 gcttgtgatt cacagcgtcc ttcagtggtg tttattaatg aggactgttt catccacgat
    13381 gcttctaaca gtcagcgcat caagcacatc attaatcaac atcccaatac gttatttatc
    13441 gtttttatgg caattgccaa tgttcatttt gatgaatatc tattggtcag aaaaaaattt
    13501 attgatcagt tctaaatcga ttaaaccgga atctctcgac gatatccttg gcgatattct
    13561 gaaaaaagag acaacgataa cctcgttttt aaatatgccg acgttatcat tgagccgaac
    13621 cgaatcgagt atgttgcgaa tgtggatggc aggtcaggga accattcaaa tctccgacca
    13681 aatgaatatc aaagccaaga ccgtttcatc gcataaaggt aatattaaac gtaagatcaa
    13741 aacgcataat aaacaggtta tctaccatgt cgtccgactg acggataatg tgactaatgg
    13801 tatttttgtc aacatgcgct aacacattct gactggtggt ttcccaccag tcaggctgaa
    13861 taagattact ctgctttctc aacaaagata ccgtcctgat gccctgcttc attaaagaac
    13921 cagatgccga gcgggtagtc ttccagcgca accaggtaca ttgtgccttc actaaactcc
    13981 tcaactgcca gtaccactcc aggacgacgc ggaccgccat ccgttttgac tgttacccga
    14041 tcattcacct tcatcgtttt cctcctgtgg ctttgtgcca gtgtagaaca atttcgtttt
    14101 tctggcagcg ccaagcgcgc gcgagtgctg attttctcga ctgtctatac ttaagagatg
    14161 ccaggcggac ttaacgactg gcggcaacaa cagagtaacg gttgcgagga aagatgatga
    14221 aaaccgcaaa agtgtacagc gataccgcaa aacgtgaggt cagcgtcgat gtcgatgccc
    14281 tgctggcggc gatcaatgaa attagcgaaa gcgaagttca tcgcagccag aacgattctg
    14341 aacacgttag cgtcgatgga cgtgaatatc atacatggcg tgaattggcg gatgccttcg
    14401 aactggatat tcatgacttc agcgtctctg aagtgaatcg ttgaatgcac aataaaaaaa
    14461 tcccgaccct gagggtgtcg ggatgaaact tgcttaagca agaagcactt aaaaaattcg
    14521 ttacaccagg aaatctgatg tgttcatcac cttatctgca atttttttcg ctgacaagaa
    14581 aatattcgcg ctatgaatga ttaacgctac ttatgaatag aaatgtgacg cgcgtcaccc
    14641 cttggtggct ctggtgtcaa cgggatcgct cccgaaaaaa tcatctttga ttatttgtgc
    14701 tgttttgaga atcatcctgg taaggggagt actgcgcgac catgttttca attcaacaac
    14761 cactactggt ttttagcgat cttgatggca ccctgctgga cagtcatagt tatgactggc
    14821 aaccggcagc cccctggctc agccgtttac acgaagccaa tattcccgtc attctctgta
    14881 gcagtaaaac ttcagcggaa atgctgtact tgcaaaaaat gttggggcta caaggcttac
    14941 cgctgattgc agagaatggc gcagtgatcc agcttgctga acaatggcag gatatagacg
    15001 gttttccacg catcatctca ggtattagcc atggcgaaat ctgccaggtt ttaaatacgc
    15061 tacgcgagaa agaacatttt aaattcacga cttttgatga tgtcgacgat gcaaccattg
    15121 ccgaatggac gggattaagc cgtagctagg cggcgctgac gcagctgcat gaggcgtcgg
    15181 taacgctaat ctggcgcgac agtgacgagc gtatggcaca atttatcgct cgtctgaacg
    15241 aactgggctt gcagtttatg cagggtgcgc gcttctggca cgtactggat gcctctgccg
    15301 gaaaagatca ggctgccaac tggattatcg cgacctatca acaattgtca ggcagacgcc
    15361 caaccacact tggcctgggc gatgggccaa acgatgcgcc tttactggag gtaatggatt
    15421 acgcggtgat tgtgaaaggg ctaaaccgtg aaggggtaca tctgcatgat gaggatccgg
    15481 cccgcgtctg gcgaacgcag cgtgaaggac cggaaggctg gcgtgaaggg ctggaccatt
    15541 ttttctccgc ccgttaagcg ttatcgctcg cgaatacccg attgcgcccg gcctgttttg
    15601 cgaggtaaag ccgacggtcg gccaatgact ggagttgttc aaaatcataa tcaccggttt
    15661 cctcgctgct acttaccccc agcgaggcac tgatgcgtat cgttgtactc ttggcgatca
    15721 gcatctcttt ttcatttaac ttcaggcgaa tacgttctgc gacctccgca gcctgcgtca
    15781 gattcgcgcc tggcagaatc acacaaaact cctcaccacc gacccgcccg gcaacgtcct
    15841 gcgcacgcaa ggaactgcta attaatccgg cagcatgaga aagaacacgg tcgcccgcct
    15901 gatgaccaaa gcggtcatta atcgctttaa aatggtcaag atcgacctga atgacagaaa
    15961 aaggatgttg gtgcgtctga cacaatttag cgagcggacg ggctttttcg aacagtgcgc
    16021 cacggttata taaacgcgtt aaggtgtcgt gccacgcctg ccactgcaac gagctttgca
    16081 gaacatacat gttgctgacc atccggcgaa tcacatacca ggaaaggagt aacatggtgg
    16141 taaagagcgc ccacagcagg gttaatgcaa tgctgatact gccgaaatcg ccgcgcacgc
    16201 cttcgcttag cgtatggacg cgcgccagca caccgtcgaa atggtccaga cgttcccagc
    16261 taacatagcg actgtccata cgaatgccgc cccgcgtgtc atgttccatt gcctgcgcca
    16321 gcaaggccag ttcacgagga tcaaaaatat tccctgttgg atggtcagga ttggaagagg
    16381 tcaaaaatat cagcttactg tcatagagct gatactcacc atcgaggttt ttatcgatgg
    16441 cgtttcttaa aaattgctgc atagtacgca cgggaatact catccccagc acgccatacc
    16501 agtagttatt actgtctacc ggaacactga cggtaacctg cggttcagta ttgctggcgt
    16561 gttccggttg cgaagtgaac cagcgtaccg cgcggtgacg attttctcgt tgcgaatggc
    16621 cgataaacca gggttgggtg acatagccgt aataacgcgt tggtacattg cgcataaaca
    16681 aggtcggctg cgtcgaaacg taaaatccag cacgcgagac atacatcgcc tgttcaacca
    16741 tcgacgagga gttgtgcgcc agtcgcagca agtaaccaac ttccagtgca gcggtaattt
    16801 cattgtcgag gctttcattt tcgcgagaca ggaaattccc ctcgctgact aatgcatccg
    16861 atacgccatt gactggcaga gtgcgccgtc ggttgagttc gatttgccag gcgtgctcgt
    16921 cgcgatgctg ttcgaactcg gttacggcat tacgcagaga ggtgaaatcc agtggcgcca
    16981 cgagagcttc gcgcatgcca ttacgcaaaa agatcagttt gtcgacgtta tactgcaaat
    17041 gcttatcgag cgcgttggct acgttttcca gatgattacg ctggctggag atataggcat
    17101 cttccagcac caccacttca cgccaggtga gcaaggtgga aaaaagcaat accacgataa
    17161 agcagagatt aacgacatga ccgggcccca ggcggcgtgc gagttttttc aaccagctct
    17221 ggttttccat ttttgtctcg tgctgcaccc tgacgactcc caacctgctt ttttatgatt
    17281 ctggcataac ccggcaatag cg
//
LOCUS       DADMEQ010000079        17238 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.080, whole genome shotgun sequence.
ACCESSION   DADMEQ010000079 DADMEQ010000000
VERSION     DADMEQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.080"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..170
                     /locus_tag="JNZ05_002666"
     CDS             <1..170
                     /locus_tag="JNZ05_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HAY8565847.1"
                     /translation="SRSRLGERPKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAV
                     FGGELTLVQLQ"
     gene            complement(171..446)
                     /locus_tag="JNZ05_002667"
     CDS             complement(171..446)
                     /locus_tag="JNZ05_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HAY8565848.1"
                     /translation="MAGYLSWLFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DIVVDVYNTEQQCLYSMSDQRIRHGGCFPIEDFIDGFWRPAQEYGDF"
     gene            complement(467..703)
                     /locus_tag="JNZ05_002668"
     CDS             complement(467..703)
                     /locus_tag="JNZ05_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HAY8565849.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(821..2260)
                     /gene="rsmF"
                     /locus_tag="JNZ05_002669"
     CDS             complement(821..2260)
                     /gene="rsmF"
                     /locus_tag="JNZ05_002669"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HAY8565850.1"
                     /translation="MAQHAVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFGGRVFGASVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPVPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2340..4973)
                     /gene="yebT"
                     /locus_tag="JNZ05_002670"
                     /pseudo
     CDS             complement(2340..4973)
                     /gene="yebT"
                     /locus_tag="JNZ05_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4942..6226)
                     /gene="yebS"
                     /locus_tag="JNZ05_002671"
                     /pseudo
     CDS             complement(4942..6226)
                     /gene="yebS"
                     /locus_tag="JNZ05_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
     gene            6356..6853
                     /locus_tag="JNZ05_002672"
     CDS             6356..6853
                     /locus_tag="JNZ05_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAY8565851.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6950..7648
                     /gene="proQ"
                     /locus_tag="JNZ05_002673"
     CDS             6950..7648
                     /gene="proQ"
                     /locus_tag="JNZ05_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HAY8565852.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7668..9716
                     /gene="prc"
                     /locus_tag="JNZ05_002674"
     CDS             7668..9716
                     /gene="prc"
                     /locus_tag="JNZ05_002674"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HAY8565853.1"
                     /translation="MSMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLERSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMMAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9908..10789
                     /gene="htpX"
                     /locus_tag="JNZ05_002675"
     CDS             9908..10789
                     /gene="htpX"
                     /locus_tag="JNZ05_002675"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HAY8565854.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10835..12208)
                     /locus_tag="JNZ05_002676"
     CDS             complement(10835..12208)
                     /locus_tag="JNZ05_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8565855.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12385..13176
                     /gene="kdgR"
                     /locus_tag="JNZ05_002677"
     CDS             12385..13176
                     /gene="kdgR"
                     /locus_tag="JNZ05_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HAY8565856.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13319..13558)
                     /locus_tag="JNZ05_002678"
     CDS             complement(13319..13558)
                     /locus_tag="JNZ05_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565857.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            13717..13860
                     /gene="mgrB"
                     /locus_tag="JNZ05_002679"
     CDS             13717..13860
                     /gene="mgrB"
                     /locus_tag="JNZ05_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HAY8565858.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13935..14222
                     /locus_tag="JNZ05_002680"
     CDS             13935..14222
                     /locus_tag="JNZ05_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565859.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14892..15035
                     /locus_tag="JNZ05_002681"
     CDS             14892..15035
                     /locus_tag="JNZ05_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HAY8565860.1"
                     /translation="MCGIFSKEVLGKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            15048..15257
                     /gene="cspE"
                     /locus_tag="JNZ05_002682"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             15048..15257
                     /gene="cspE"
                     /locus_tag="JNZ05_002682"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY8565861.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15423..16232
                     /gene="rlmA"
                     /locus_tag="JNZ05_002683"
     CDS             15423..16232
                     /gene="rlmA"
                     /locus_tag="JNZ05_002683"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HAY8565862.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16229..16795)
                     /gene="mntP"
                     /locus_tag="JNZ05_002684"
     CDS             complement(16229..16795)
                     /gene="mntP"
                     /locus_tag="JNZ05_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HAY8565863.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGSDDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4555 a         4169 c         4346 g         4168 t
ORIGIN      
        1 gtagccgctc gcgattaggt gaacgcccaa aagggcaggg aattacaggt gctgatcatt
       61 tctggtttgg tcatacaccg ttgcgacatc gcgtggatat tggcaacttg cattatattg
      121 ataccggtgc tgtctttggg ggcgaactga ctcttgtgca actgcaataa ttaaaaatca
      181 ccatactcct gtgctggtcg ccagaaacca tctataaaat cctcaatcgg aaaacaaccg
      241 ccatggcgga tccgttgatc gctcatagaa taaagacact gctgttccgt gttgtagaca
      301 tccacaacaa tatcttcaca accgccatcc aggtagcaaa caaaaagtac cagcgcgaac
      361 atttcatccc cgaagtgtgg tgccgtaccg ttaagtttag gagagatttt acaacggggg
      421 aataaccagg acaaataacc cgccatgccg acgggttctt tttggatcag gcaagacgca
      481 taatccaggc atcagattgt tgatcgtaac gggtacggta aaggacagag tgttcgccat
      541 ttagcagggc agggatactg gtttcagcat cccaggcatc cagttgcata cataaatcgg
      601 ggtcagattt acaaggaatg gttaacgttc gatcacccgg cgattcaccg tgtaattcgc
      661 cgtcgtcgat ttcaaaagcg cctatgcgca cactggtttt catcttcgtg ctctccattt
      721 agctgctgat tgtggtacga ccagtccgat cgcacaattt ttcagcctag ttgaggccgc
      781 cgaatgtgcc agtcaaaaag tgcgcttttt ttgtgcgctg tcatgcgtta ctggtaaaaa
      841 gtttcccgtc ccgcaccagt tcacgcgggt agctgttttt cagtcgcgaa ccaatccgtt
      901 tggctaaacc aatcggctga tgctggaaag taaccaacac gtcatccgcc actggcgcgg
      961 cttgcgggta aacatcgcgc ccgcgatacc actcctccgc ttcctgcggt gtcagttcaa
     1021 aagcgtttac attgtcgggg gaggcatggg caataaccgc ttcatgctgc cagcgataac
     1081 ctttgttatg cgtttcggca agtttaatcc ctaagcgaga aaatcggact ttaccgatca
     1141 gggcttcaat gcctaccggg aacaaccaca gttctttgtc gcgttgccag agtcgcaggt
     1201 ttccatccca gtttaagcca acacttgcag ccgcctgacg aatttgtccg gcttcgcgat
     1261 ctttcaccgg gctgaacggg aaattgccca ctttgtattt tgggacgggt aaggcgggga
     1321 tcgcctgagt tttacgcaga cgagcaacga agaagccttc gcagtcgtaa atttgtggga
     1381 aaacatgcaa aaaaccttct tcggtcagcg ccttgtttgc accagggaag agatcgccaa
     1441 gtggtaaaaa ctctacttcg tcggggtaag tctctttcag ccaccggcaa acggcttcgt
     1501 tttcttcccg gtttaaggta caggtcgagt aaaccagcgt accgccagga cgtaatgcat
     1561 gaaaggcgct gtcaatcagt tcccgttggg tagctgcgat ttcctgattg ctttcgggtg
     1621 accagttttt tagcgcatcg ggatctttac gcaccacgcc ttcgccggag cagggagcgt
     1681 ccagcaaaat ggcatcaaac atttctggca ctgacgcacc aaacacgcgg ccaccaaaat
     1741 gtgtgagcgc aacattactg atgccacagc ggctgatatt ggcatgtaac acttttaccc
     1801 gactggcgga aaactcattg gcaaggattg ccccttcgtt attcatccgc gcggcaattt
     1861 gcgtcgtttt tgagcctggc gcagcagcga catccatcac ccgctgtggt gcattaccgt
     1921 cagcaaacaa ggcggcgacg ggcaacattg aactggcttc ctgaatataa aacaggccac
     1981 ttaaatgctc ggtggtacta cccaatggca atgcatcttc attgtcgcgt tcaatccaga
     2041 aaccttcttc acaccacgga attggcgtaa gcgtccagcc ataaggagcg gttaattgca
     2101 ggaaatcagc aacggagatt ttcagcgtat taacgcgaat gctgcggcgc aacgggcgct
     2161 gacaggcggc aagaaaatca tcaaatgaga gcgtcgaagg catagcttcg cgcatttgtg
     2221 tcagaaacgc gtccgggaaa taaacggcgt gttgggccac gagcatgtac cactggcaaa
     2281 aaaacaggcg cgcagtttag cataaacgct ccggcgcagg cacgccggag cagtgggcat
     2341 tatttgggaa gcgcagttcc ccattcacgc cactctttcg gttcactttc ctgcaacagg
     2401 aagtgtttgc cttcctgggc tttcggtgca agtggcgtac ccggaggcgt ggcgaaggcg
     2461 atgccgccac ggataaattg gttaaaggtg ccggttttca ctacgccgcc cgtcagacca
     2521 aagtccagac tgtaacccga tgccaaccag aagacggaat tgttacgcac caggtgttga
     2581 tagcgtttac tgatgcgcat cgcaatcatc acgcgatctg acaatgtccc cagcgtcatt
     2641 ccggtaaccg tacctacttc cagaccacgg aacagcacag gcgtaccgat gcctaacgaa
     2701 ccggcttccg gcgcttcaac aataatgctt aaaccatcta agtaacgcga atcagtaatg
     2761 gtggcctctt ctaattcaaa gtcgcgacga ggattgccac ggcctggttc gacgttgata
     2821 tacggctgga ggatagtatc tagatgctca acgccagctg ccgagatttg cggtgtgacc
     2881 actgagaagc gcgtaccacc gcgagcaaag gtctggacat attccggata gagcaccgcc
     2941 tttgcctgta cttcattgcg tgcggtaatc agatccagcg tctggatttg cccgatatca
     3001 ataccgagat agcgaattgg catcccgacc gccagttttc cggcatcgaa agcgtgaagc
     3061 gtaatctgcc tgccaaccgc acgggccgct gtttcggaag catacagaat tcgcttgtcg
     3121 cctttacgct gactggcgct ggcaccactg aggttatcga agctaatggc cccctttaat
     3181 gctctggaga gcggggatgc ctgtacggtc aggccactac cattcagctg aactttcgcc
     3241 ccgccttctg cctagaacac gctgttgctg gtcaaaaggt tgcgatattc cggtttaatg
     3301 tgcagatcga tatcgaacgc gttagctcgc ggacgcacgg taataacttc accaacttca
     3361 aatttacggt acagcactac cgatcctgcc tgcacatccg gcagcgtctc tgcactcaaa
     3421 ctcacggttg tggtgggtaa atcgctaagg ctgttctcca gcgctttttc cagattggca
     3481 tacagtggat agctggcttt catctcgcct ttatcgcccg gcagaatacg tatcccgccg
     3541 ttaatccatt ctgaggcgct ggcaccgaga aactcaacgc catccagccc caccttcacg
     3601 tcgacacggc tgttgacgac aaatttgcta tcgcctttta ccagttctcg atgctgaggc
     3661 tcgatggcga cggtaaaggt gacgcctttg ctggtgagtt tacgatcgat aacctggcct
     3721 acctgcacgc cgtgaagaat gagcggctga cccgcatcaa taccgtaact ttccggtgct
     3781 gtcagggtca gtgtcagaac gtccggctct tgcagcaggg ctttttcgcc cggcacaaca
     3841 acgaactctt tgcgtggctc gccatcgccg ggcaccaact caaaggtttt tccggtcagc
     3901 agggcgctga gattagcatc gctaagggat aatttcgggt tgcgtaattc tatgcgggta
     3961 ttttcacgaa gcagggtaac gacgctggga tcaacggtca tttccccagt gactttacca
     4021 ccaggattta aatccagttt agtcagctgt ccgacttcca gcccctgata cattaacggc
     4081 gtcgagtcgg cggttaatcc tgctccactc ggcagttcca gttttattat tactccacgc
     4141 tggctgtggg ccagatcttc atacagacca aaggtatctt ccgcctcggc aggtttcgac
     4201 tcttcaggtg aatcgaaggc aatcgcaccg ttaaccagtg ccgccagact ttccagtttc
     4261 accttcgcgc cactgatact gacgttggca tcaacgccgg aaacgttcca gaaacggcta
     4321 ccttttttca ccagatcggt aaaacgccgc tcgatcagga catcaatcac cacgccttgc
     4381 ttgttgggat tgatggcata gtcgtagact tttcccaccg ggatcttgcg gaaatagacc
     4441 aatgaaccgc tgttcagcga accgagatcg ggggcttgca ggtggatcat cagatcgcca
     4501 ttgtccagcc gatatttcgg ttgggtatcg agcgcgacaa agtgatcctg ctctttacct
     4561 ttacccggca tcatgccgat atagttccca ccgacgaggg cgtccagccc ggagacacct
     4621 gccaacgatg cttttggcgt catcagccag aactgagtct cttcgcgcag cgcatctttc
     4681 atatcggact tgatgctgac cttgacttca atcttacgaa gatcgtcgct gaggctgata
     4741 tcctgcactg ttccgacttc aacgccctga taacgaacag gcgtacggcc cggaacaata
     4801 ccatccgccg acataaagtc gatggtgacg gtattacccc ggtcctgata actgtcccaa
     4861 atcagccaac cggcaatcat tagcgcgatg aaaggcagca gccagaaagg tgagatacgg
     4921 cgtttatttt taatctgcgc ttcagtcgtc gaagcgggcg tttcctgact catgtgcatc
     4981 ccaaagtaag cggctgtcca gccattccac agcaagaata gtcaaaatta cggcagcgcc
     5041 aaaataaaac gcagccggtc ccatagtaaa agcgaggatc tgatcgcgat taatcagcga
     5101 catggttaaa gatatgacaa acaggtcgag catcgaccag cgtccaatcc aggtcaccat
     5161 ccgcagtaac agaatgcgtg tgcgtaaacc ttgctggcat ttaaaatgaa tgctcaacag
     5221 taaagtgaac atgacgatca ctttagtaaa cggtaccaga atactggcga taaacacgat
     5281 tcccgcaacc gcaatgttgc tacttgccag cgacataatt ccggaaagaa tcgtatcttc
     5341 ctgccggcca ccattcagat aaatgataga aataggcaat aggttggctg gtaacaacaa
     5401 aacgattgac gctaacagcg ccgcccagca tttttgcaaa ctatgacgac gacgcaggcg
     5461 tagcgggata tggcaacgcg ggcagagacc acgctgatca ggatagccgg taaaatggca
     5521 cccaagacag acacgaagtt tctcgtccct acgcgtagcg gggcgctgcg gataatatcg
     5581 ctcccacagc tcctcaacat taagatgtga caacgtcacc gtcgttaaaa tcaccaacgc
     5641 cacaaaagag aacaagccga cacccgcctg gatatgggca taatcctgta ccttatatag
     5701 aagcaacgcc aatgccgacc aggtagatat ccagcattac ccactcttta agtcgctcaa
     5761 gcatcagcag taccggacgt aaattcattc ccagtcggtt accaaaccat aaatatgcta
     5821 tggaggacac cagaatgagg ggggcaccaa taacgcagaa aaagaccatt gatcccgtta
     5881 tcgcatcgcc ctgtttggtc atttgccaga tgccttgcat gacgttggcg tcaatacgaa
     5941 tgcctaacag ccagatatgc aacagcggtt cgccccaggc aaacggcata agcaacagca
     6001 tggtgaaggc cattgccgcc aggcgcgtta gcgacaagtc gcgcccgtcg cgaatttttg
     6061 cctgacagcg cggacaatag gcactttgat gagaatttat ctcgggcagg ctaaacagca
     6121 tgtcacattg cgggcaacgt tggtaatcac cacgcggcag ttcctcgccg attgccctca
     6181 ctgttatctt ttttgtcggc gtaatttgtg gtgtgttaag agccatgacg tgccaaatgt
     6241 aataagaatt aacattaatc ttaacccatg atgggaatga tcttgagcat taacgcattt
     6301 aatgcttatt ttaataggct ggcagttcca ggtaagacaa ccaagtgatt aaataatgaa
     6361 caaaacagaa ttttacgcgg atttaaatcg cgactttaac gcgctgatgg cgggagaaac
     6421 cagttttctg gcaacgcttg cgaacaccag tgcgttgtta tacgagcgtc tcactgacgt
     6481 taactgggca ggtttttatt tgcttgagga cgatacactg gtactcggac catttcaggg
     6541 caaaattgcc tgtgtccgga tacccgtcgg gcgcggggtg tgcggcacag cggttgcccg
     6601 caatcaagtg cagcgtatcg aggatgttca cgcgtttgac gggcatattg cctgtgatgc
     6661 ggcgagtaat tctgaaattg ttctgccgct ggtggtgaaa aatcagatta ttggtgttct
     6721 cgacatcgat agtaccgtct tcgctcgctt tacagacgag gacgagcaag gcttacgtca
     6781 gcttgtggca cagcttgaaa aagtgcttgc aacgacggat tacaaaaaat tctttgcgag
     6841 cgtcgcagga taatcaacgg ataacgtagc atttactggt agcgtcatta taatgacgcc
     6901 tgttcatgcc tgcgcttgtt ggctacgtcc gttgtaatca ggaaatttca tggaaaatca
     6961 acctaagttg aatagcagta aagaagtaat cgcgtttctc gccgaacgtt ttccccactg
     7021 tttcagtgcg gaaggtgaag cgcgtccgct gaaaattggt atttttcagg atttggtcga
     7081 tcgtgttgcc ggggaaatga acctgagcaa aacgcaatta cgatccgctt tacgtctcta
     7141 cacttcgagc tggcgttatc tttacggtgt taaacccggc gcaacgcgtg tcgatcttga
     7201 cggcaaccca tgcggtgagc tggacgagca acatgtagag catgctcgca agcagcttga
     7261 agaagcgaaa gcgcgtgttc aggcacagcg tgctgaacag caagcgaaaa aacgcgaagc
     7321 tgccgcagct gctggtgaga aagaagacgc accgcgccgc gaacgcaagc cacgtccgac
     7381 cacgccacgc cgcaaagaag gcgctgaacg taaacctcgt gcgcaaaagc cggtagagaa
     7441 agcgccaaaa acagtaaaag cacctcgcga agaacagcac accccggttt ctgacatttc
     7501 agctctgact gtcggacaag ccctgaaggt gaaagcgggt caaaacgcga tggatgccac
     7561 cgtattagaa atcaccaaag acggcgtccg cgtccagctg aattcgggta tgtctttgat
     7621 tgtgcgcgca gaacacctgg tgttctgaaa cggaggccgg gccaggcatg agcatgtttt
     7681 ttaggcttac cgcgttagct ggcctgcttg caatagcagg ccagaccttc gctgtagaag
     7741 atatcacgcg tgctgatcaa attccggtat taaaggaaga gacgcagcat gcgacggtaa
     7801 gtgagcgcgt aacgtcgcgc ttcacccgtt ctcattatcg ccagttcgac ctcgatcagg
     7861 cattttcggc caaaatcttt gaccgctacc tgaatctgct cgattacagc cacaatgtgc
     7921 tgctggcaag cgatgttgaa cagttcgcga aaaagaaaac cgagttaggc gatgaactgc
     7981 gttcaggcaa actcgacgtt ttctacgatc tctacaatct ggcgcaaaag cgccgttttg
     8041 aacgttacca gtacgctttg tcggtactgg aaaagccgat ggatttcacc ggcaacgaca
     8101 cttataacct tgagcgcagc aaagcgccct ggccgaaaaa cgaggctgag ttgaacgcgc
     8161 agtgggacag taaagtcaaa ttcgacgagt taagcctgaa gctgacagga aaaacggata
     8221 aagaaattcg tgaaaccctg acacgccgct acaaatttgc cattcgtcgt ctggcgcaaa
     8281 ccaacagcga agatgttttc tcgctggcaa tgatggcgtt tgcgcgtgaa atcgatccgc
     8341 ataccaacta tctttccccg cgtaataccg aacagttcaa cactgaaatg agtttgtcac
     8401 tggaaggtat tggcgcagtg ctgcaaatgg atgatgacta caccgttatc aattcgatgg
     8461 tggcaggtgg tccggcagcg aagagtaaag ctatcagcgt tggtgacaaa attgtcggtg
     8521 ttggtcaaac aggcaagccg atggttgacg tgattggctg gcgtcttgat gatgtggttg
     8581 ccttaattaa agggccgaag ggcagtaaag ttcgtctgga aattttacct gctggtaaag
     8641 ggaccaagac ccgtactgta acgttgaccc gtgaacgtat tcgtctcgaa gaccgcgcgg
     8701 ttaaaatgtc ggtgaagacc gtcggtaaag agaaagtcgg cgtgctggat attccgggct
     8761 tctatgtggg tttgacagac gatgtcaaag ttcaactgca gaaactggaa aaacagaatg
     8821 tcagcagtgt gatcatcgac ctgcgtagca atggcggtgg ggcgttgacc gaagcggtat
     8881 cgctctcagg tctgtttatt cctgcgggtc ccattgttca ggtccgcgat aacaacggca
     8941 aggttcgtga agacagcgat accgacgggc aggtgttcta taaaggccca ctggtggtac
     9001 tggttgaccg tttcagtgct tcggcttcag aaatctttgc cgcggcaatg caggattacg
     9061 gtcgtgcgct ggttgtgggt gaaccgacgt ttggtaaagg caccgttcag caataccgtt
     9121 cattgaaccg tatttacgat cagatgttac gtcctgaatg gccagcgctg ggttctgtgc
     9181 agtacacgat ccagaaattc tatcgcgtta acggcggcag tacgcaacgt aaaggcgtaa
     9241 caccagacat catcatgccg acgggtaatg aagaaacgga aacgggtgag aaattcgaag
     9301 ataacgcgct gccgtgggat agcatcgatg ccgcgactta tgtgaaatca ggagatttaa
     9361 ctgcctttga accggagctg ctgaaggaac ataatgcgcg tatcgcgaaa gatcctgagt
     9421 tccagaacat catgaaggat atcgcgcgct tcaacgctat gaaggacaag cgcaatatcg
     9481 tttctctgaa ctacgctgtg cgtgagaaag agaataatga agatgatgcg acgcgtctgg
     9541 cgcgtttgaa cgaacgcttt aaacgcgaag gtaaaccgga gttgaagaag ctggatgatc
     9601 taccgaaaga ttaccaggag ccggatcctt atctggatga gacggtgaat atcgcactcg
     9661 atctggcgaa gcttgaaaaa gccagacccg cggaacaacc cgctcccgtc aagtaatatc
     9721 aatcaggcac aagaaattgt gcctgatttt ttaacagcgg caagatgccg taaatcagat
     9781 gctacaaaat gtaaagttgt gtctttctgg tgacttacgc actatccaga cttgaaaata
     9841 gtcgcgtaac ccatacgatg tgggtatcgc atattgcgtt ttgttaaact gaggtaaaaa
     9901 gaaaattatg atgcgaatcg cgctcttcct gctaacgaac ctggccgtaa tggtcgtttt
     9961 cgggctggta ctgagcctga cagggataca gtcgagcagc gttcaggggc tgatgatcat
    10021 ggccttgctg ttcggttttg gtggttcctt cgtttcgctt ctgatgtcca aatggatggc
    10081 attacgatct gttggcgggg aagtgatcga gcaaccgcgt aacgaaaggg aacgttggct
    10141 ggtcaatact gtagcaaccc aggctcgtca ggcggggatc gctatgccgc aagtggctat
    10201 ctaccatgcg ccggacatca acgcttttgc aaccggtgcg cgccgtgatg cctctctggt
    10261 tgctgtcagc accggtttgc tgcagaacat gagcccggat gaagccgagg cggtaattgc
    10321 tcacgaaatc agccacatcg ccaatggtga tatggtcacc atgacgctga ttcagggcgt
    10381 ggtgaacacc ttcgttatct ttatttcccg tattctggcg cagcttgccg cgggttttat
    10441 gggcggaaat cgtgatgaag gtgaagagag caacggcaac ccgctgatct actttgcggt
    10501 tgcaacggtt ctggaactgg tgtttggtat tctggcgagc attatcacca tgtggttctc
    10561 gcgtcatcgt gaattccatg ctgatgccgg ttcggcaaaa ctggttggtc gcgagaaaat
    10621 gattgccgcg ctgcagcgcc tgaaaaccag ctatgaaccg caagaagcaa ccagcatgat
    10681 ggctttctgc attaacggta agtcgaaatc gctcagtgag ttgttcatga ctcacccgcc
    10741 gctggataaa cgtattgaag ctctgcgtac gggtgaatac ctgaagtaat atgaagtaaa
    10801 tactaaaaag cgcgtcctga tcgacgcgct ttttttatgc cctggctcgt ggctgagtga
    10861 tacgtaaacc actgacacag gcagcaatca ctgccagaat agccgcagcc atcagcgaga
    10921 cgtgcgtacc attatcacca aactgattta gcatcagcgc caccagcgcc gcgccactac
    10981 tctgacccag tagacgagcc gttcctaaca tgccactggc cccaccgcta cgttcgcgcg
    11041 gagcggaggt aataatggtg tggttattgg gtgactggaa taagccaaat ccagcgccac
    11101 ataagatcat cggccagata atattgatat ccgcaggtga cgcgggcagc agaaccaggg
    11161 aaaaaagccc cgcagccatg atgaacaacc ctaaagcacc cagcaatcct gcatgtacgc
    11221 gttcaatcaa atagcctgcc agcggagcca tcaccatcgt tgctaacggc cacggtgtca
    11281 gaagtaaacc tgtttcgact tcactacgcc cgagcacggt ttgcaggtaa aagggcaggg
    11341 aaaccattgc cagcatttgt gcgcagaaag agcaaacaga tgtgcaaata gaaagtgaaa
    11401 acagcgggat acgcagtaaa tccaccggta gcagcggtac gggaagagaa agctggcggc
    11461 gaataaagaa aataccaaca acaaccatta ccaccagttc cgcagcaatt aacgtcagcg
    11521 attgcccctg agcgaaacca ctcaacgcag tgataagcag gccgaaggtt aacgcgttca
    11581 tcacggcgct gggcaggtcg aaacggggtt tactggcgcg agaaccattg ggtggcagaa
    11641 aacgcatcgc cagaagcagg gcgataatac ctaacggtac gttgattaaa aataaccatt
    11701 tccaggatgc gatggagagg attgctgcag caattgtcgg cccggcagca gaagagacgg
    11761 caacaataaa cgagtttatg cccatccctc tccccagaaa acgttgtgga tagatcaggc
    11821 ggataagtgc ggtattaacg ctcatcaacg ctgcaccgcc gaaaccttgt atgacacgcg
    11881 caagggtgag catttgcagc gaatcagaaa gggcgcagaa cagtgaagac aacagaaaaa
    11941 cgaccagacc gcatttataa atacgtcgat agccaaacat atcgcccaga aacgaaaacg
    12001 agagcaggga gatgacaatg gcgatttgat aggcgttcac tacccagatg gaactggctg
    12061 gtgtggcatg aaggtccgtg gcgattgttg gcagggcgac gttggcgatt gcgccgtcaa
    12121 ggacagccat cgaaatacca atcacaatgg ttaatatcgc accgtatcgc tggggcaatg
    12181 gcaggccgtc ggcctgaact tttggcatag gaattttata tctttggtga ataatcaaca
    12241 ggggtatgat tctagcactg atattcacgg tggatgtcgc agaattgtaa ctaaccggca
    12301 aaagatggat tgcgaccacc ctggtgcgaa attataataa aaaacagttc tgatttttat
    12361 aaaacactcg caatgaggtg ataaatggct aacgcagatc tggataaaca gcctgattct
    12421 gtatcttccg tgctaaaagt ttttggcatt ttgcaggcgc tgggtgaaga gcgcgaaata
    12481 gggataaccg agctgtcgca gcgcgtcatg atgtcaaaaa gcaccgttta tcgcttttta
    12541 cagaccatga aaaccttagg ttatgtggcg caggaagggg agtcggagaa atattccctg
    12601 accctgaaat tgtttgaact gggcgctcgc gcgttacaaa acgtcgattt aattcgtagc
    12661 gcagatatcc agatgcgtga gatctcccgc ctgaccaaag aaactatcca cctcggcgca
    12721 ctggacgaag acagtattgt ttacattcac aaaattgact ctatgtacaa tttgcgcatg
    12781 tattcacgga ttgggcgtcg taatccgctg tacagcaccg cgattggtaa ggtactgctg
    12841 gcatggcgcg atcgcgatga agtgaagcaa attcttgagg gcgtggagta taaacgcagt
    12901 accgagcgga ccatcaccag tacagaagcg ttattacccg ttctggacca ggtgcgcgag
    12961 caggggtatg gcgaagataa cgaagagcag gaagaagggc tgcgatgcat tgcggtaccg
    13021 gtatttgatc gctttggcgt ggtcattgcc ggtttgagca tctccttccc gacgttgcgt
    13081 ttctctgaag agcgtttaca ggaatatgtc gcaatgttgc ataccgcagc gcgcaaaatt
    13141 tctgcccaaa tgggttatca cgactatccg ttctgatgag agtaagaacc tgtcggaata
    13201 tcaaacagac aggttcttta tttagcatga gaaaaataaa gttgaaggtg cgttatatta
    13261 aacgcgcttg ctataagaat attttactca tgagtgagaa tctggttatt tattaccctt
    13321 aaccattatc gaccacgata ttgcttttac gtaacagtgg gcaatctgtt atccccaaga
    13381 aaccactttt agtgtgcaag tattgtaccg tgctggtgcc tctggcagtc agataggtac
    13441 attgcaaacc taatcctgcg gcattctctt tgcttccaat caaaacgcca tatccgctga
    13501 gtaataatcc tatccatacc agtgctatca gcataactgt gcgaatgatg aatcgcatta
    13561 caatctcttc tctttttatg ttcgcttaat cgtagcggca atatgcgctg aagcaagcta
    13621 ctcattccga aaaagcacga atatcgacat agttaggcgc tgtttaacta acgcatgcta
    13681 gtttaatgac ataaggtagg tgaagcggag attggagtga aaaagtttcg atgggtcgtt
    13741 ctggttgtcg tggtgttggc ttgcttgctg ctttgggcgc aggtattcaa catgatgtgc
    13801 gatcaggatg tacaattttt cagcggaatt tgtgccatta accagtttat cccgtggtga
    13861 tatcattttt tcagcactag attttcttcc tctccagcag tggtagaata gcgcctctac
    13921 tcagaggtgg tgaaatgaac gaagttgtaa attcaggcgt gatgaacatt gcgtctttgg
    13981 ttgtatcggt ggtggttctt cttatcgggc tcatcttgtg gttttttatc aatcgtgcca
    14041 gttcacggac taacgaacag attgaactgc ttgaggcgtt gctggatcag caaaaacgtc
    14101 aaaatgcact gttacgtcgt ttgtgcgaag caaacgaacc agagaaagca gataaaaaga
    14161 ccattgagag tcaaaaatcg gttgatgacg aagatattat tcgcctggtc gccgaacgat
    14221 aatccttcct caatcttgag taaccccgtc gttgaagggt tactcaattc atcctgatta
    14281 tacgcactgt catggtgcaa ctgcttcaaa ttaaaactct ggtctgactt caaataatta
    14341 tcatattatt tatttgcgta acgggatatc agaaaaagat attttcctca ttaatttatt
    14401 aggatgttta catcagattt gtgattaagc gtggtattat ttattagcga aacgtttctc
    14461 tcttgatttt tttgcgcact catcaatttt tcttatttta aatttacaat ccctttgggg
    14521 attgacttct ctttagggta attgatagcc gttaactgac tgttttatga gaaaaagtga
    14581 tataactttt tattcattgc atagcagaaa atgtgatatt gcacgcacta tgtaataact
    14641 tctccctctg gcctggaaca actgaactta ttgaactatg ttagaaacta cgccagttta
    14701 agtatctgcc tgaactggca aggttaagca caatgatata tcggcgcgta ttccgttgca
    14761 taagtgtgca aaaaaagtgg aagacgtatc gagatttgtg cgtctgatcg agacatgttt
    14821 aaaaatggct tgccataatt aacgttgtat gtgataaaag atttcgggtt aaacgaggta
    14881 cagttctgtt tatgtgtggc attttcagta aagaagtcct gggtaaacac gttgacgttg
    14941 aataccgctt ctctgccgag ccttatattg gtgcctcatg cagtaatgtg tcagttttat
    15001 ctatgttatg cctgcgggcg aagaaaacaa tctaaggaat ttttcaaatg gcaaagatta
    15061 aaggtcaggt taagtggttc aacgagtcta aaggttttgg cttcattact ccggctgatg
    15121 gcagcaaaga tgtgttcgta cacttctccg ctatccaggg taatggcttc aaaactctgg
    15181 ctgaaggtca gaacgttgag ttcgaaattc aggacggcca gaaaggtccg gcagctgtta
    15241 acgtaacagc tatctgatcg aatccactga tctgaagtgt gaatacgctt caatctcgct
    15301 ataaagcctc gtcgaatgcg aggcttttta ctatgcttta tcttcgctcc tggcgttcgg
    15361 atatttgcca gccacgtgat tcgcgttaca cttgcggcct ttagtatcct gccggagttg
    15421 tcatgtcgtt ttcctgtcca ctttgccatc agcctctttc gcgtgaaaaa aacagctata
    15481 tctgtcccca acgacatcag tttgatatgg cgaaagaagg gtatgtcaat ctgctgcccg
    15541 ttcagcataa acggtctcgt gatccgggcg acagcgcgga aatgatgcaa gcacgccgcg
    15601 cattcttaga tgccggacat tatcagccgc tgcgtgatgc aattgtcgcc caactgcggg
    15661 aacggcttga tgaaaaggcc acggcggtgc tggatattgg ctgtggtgaa gggtattaca
    15721 cacacgcatt tgccgatgcg ttgcccgaaa tcaccacgtt tggcctggat gtctcgaagg
    15781 tagcgataaa agcggcggcg aaacgctatc cgcaggtcac tctttgtgtc gcttccagcc
    15841 accgtttgcc gttttccgat accagtatgg acgccataat acgtatttac gcgccgtgta
    15901 aagcagaaga attagcacga gtagtgaagc ccggcggctg ggtcattact gccacgccgg
    15961 gaccgcgaca tttgatggag ctgaaggggc tgatttacaa tgaagtacat cttcatgcac
    16021 ctcatgcaga acaactggaa ggttttacat tacagcagag tgcggagttg tgttatccga
    16081 tgcgtcttcg cggtgatgaa gccgtcgcat tattgcagat gacgccgttt gcctggcgtg
    16141 cgaagccaga agtctggcaa acactggcag caaaagaagt gttcgactgc cagacggact
    16201 ttaatattca cctctggcag cgttcttatt aaccgtggaa gtgcgtccag aggatctgga
    16261 cgccgatgcc gatcagcacc agcccgccga gaatttccgc ttttttccca ataattgagc
    16321 cgataaagcg accaaccatc atccctaatg tcgacataat caaggttgca caaccaatgg
    16381 ccaatgcggt cgcgataatg ttgacctgaa ggaaagcaag accaacaccc acagccatgg
    16441 catccaggct ggtggcaatc gcggtggtta ccagtagcca gaaaccgtgc cgacggcgcg
    16501 gctcttcatc ttcatcatct gagccacgaa aaccctcaat aatcattcgc ccgccgagga
    16561 atatcagcag cacaaacgca atccagtgat tccattcaag gacaaaccgg ctggctaaca
    16621 tgcccattcc ccagccgatc agcggcgtca gggtttcgac ggcaccaaaa ataaggccgg
    16681 ttcgcaatgc ttcagaaaat ttcggtttat ggaggggggc acctttaccg attgatgcag
    16741 caaatgcatc catcgacata ccaaacgcaa gaagaacagt agcagtgata ttcatgacaa
    16801 cgtcctgacc ggggtatcca taaaacacat catcgccccc agcaaacatg catacatgaa
    16861 caacaagccg cgatgggttt tcgcgttcat gcaggcatag cgattgatgt gcatatggtc
    16921 tcgcctgact gaaagtcttc agtccgtacg tgccacgttt tgcaacgagt atgttgacac
    16981 gtacatttcc ggaatctgga aatcggctac tccccaaatg agggcgcaac cttaacatat
    17041 tttgagaata taaaacaact gttggaatat attatttcat tgctttattg ataacgattt
    17101 tcatttatgt ttatatatca atgaaacgtt aaaataaata acaaataagg gaagagataa
    17161 tatagcctaa gctatgtctg attattaaaa atacactaaa catagctttg gctaaattca
    17221 taagttattg agttgata
//
LOCUS       DADMEQ010000080        17183 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.081, whole genome shotgun sequence.
ACCESSION   DADMEQ010000080 DADMEQ010000000
VERSION     DADMEQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.081"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(329..673)
                     /locus_tag="JNZ05_002685"
     CDS             complement(329..673)
                     /locus_tag="JNZ05_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY8565864.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            805..2715
                     /locus_tag="JNZ05_002686"
     CDS             805..2715
                     /locus_tag="JNZ05_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAY8565865.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSLDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2773..3468
                     /gene="tsaB"
                     /locus_tag="JNZ05_002687"
     CDS             2773..3468
                     /gene="tsaB"
                     /locus_tag="JNZ05_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAY8565866.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3508..4089
                     /gene="yeaY"
                     /locus_tag="JNZ05_002688"
     CDS             3508..4089
                     /gene="yeaY"
                     /locus_tag="JNZ05_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HAY8565867.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4294..5979
                     /gene="fadD"
                     /locus_tag="JNZ05_002689"
     CDS             4294..5979
                     /gene="fadD"
                     /locus_tag="JNZ05_002689"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HAY8565868.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            6061..7176
                     /gene="rnd"
                     /locus_tag="JNZ05_002690"
     CDS             6061..7176
                     /gene="rnd"
                     /locus_tag="JNZ05_002690"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HAY8565869.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRADWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMFNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHRKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="JNZ05_002691"
     CDS             complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="JNZ05_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HAY8565870.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8251..8853)
                     /locus_tag="JNZ05_002692"
     CDS             complement(8251..8853)
                     /locus_tag="JNZ05_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-hydroxylating oxygenase subunit alpha"
                     /protein_id="HAY8565871.1"
                     /translation="MLEACPEVHNLKLAARFTTRTPANWKNIVDNYLECYHCGPAHLG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKQSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTNEQKSLIEWYRDVFHPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            complement(9210..9761)
                     /locus_tag="JNZ05_002693"
     CDS             complement(9210..9761)
                     /locus_tag="JNZ05_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8565872.1"
                     /translation="MRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTYPSLDR
                     LAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELLEDKEL
                     HSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRKKILSK
                     YGIYEQEGLVAPSQEPQDVKKAV"
     gene            9987..10214
                     /locus_tag="JNZ05_002694"
     CDS             9987..10214
                     /locus_tag="JNZ05_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8565873.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSREAGLKPDTMRNVFRC
                     HIPRYEAIIARYLDMEPAQIWPSRYMEKTSC"
     gene            10208..10639
                     /locus_tag="JNZ05_002695"
     CDS             10208..10639
                     /locus_tag="JNZ05_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565874.1"
                     /translation="MLTHWVTAQECVGMSCFPKHEVSIRRNLEKLAANHPELRRKRTG
                     SKVLEFHISVLPAAARVELLLKSEQIETSQGYFEIARPTLEAHDYDREALWSKWDKAS
                     DSQRRLAEKWLPAVQTADEMLQPLLVRLSCYLSTGRLKVNA"
     gene            complement(10691..10765)
                     /gene="yoaJ"
                     /locus_tag="JNZ05_002696"
     CDS             complement(10691..10765)
                     /gene="yoaJ"
                     /locus_tag="JNZ05_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HAY8565875.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(10768..10866)
                     /gene="yoaK"
                     /locus_tag="JNZ05_002697"
     CDS             complement(10768..10866)
                     /gene="yoaK"
                     /locus_tag="JNZ05_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HAY8565876.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            complement(10899..11924)
                     /gene="dgcP"
                     /locus_tag="JNZ05_002698"
     CDS             complement(10899..11924)
                     /gene="dgcP"
                     /locus_tag="JNZ05_002698"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HAY8565877.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            12107..12361
                     /gene="yoaF"
                     /locus_tag="JNZ05_002699"
     CDS             12107..12361
                     /gene="yoaF"
                     /locus_tag="JNZ05_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HAY8565878.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            complement(12383..12730)
                     /locus_tag="JNZ05_002700"
     CDS             complement(12383..12730)
                     /locus_tag="JNZ05_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HAY8565879.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(12785..13966)
                     /gene="nimT"
                     /locus_tag="JNZ05_002701"
                     /pseudo
     CDS             complement(12785..13966)
                     /gene="nimT"
                     /locus_tag="JNZ05_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            14063..14884
                     /locus_tag="JNZ05_002702"
     CDS             14063..14884
                     /locus_tag="JNZ05_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8565880.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            complement(14841..15287)
                     /locus_tag="JNZ05_002703"
     CDS             complement(14841..15287)
                     /locus_tag="JNZ05_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAY8565881.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            15457..15561
                     /locus_tag="JNZ05_002704"
     CDS             15457..15561
                     /locus_tag="JNZ05_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565882.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            complement(15562..16065)
                     /gene="yeaK"
                     /locus_tag="JNZ05_002705"
     CDS             complement(15562..16065)
                     /gene="yeaK"
                     /locus_tag="JNZ05_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HAY8565883.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNTGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(16108..17166)
                     /locus_tag="JNZ05_002706"
     CDS             complement(16108..17166)
                     /locus_tag="JNZ05_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY8565884.1"
                     /translation="MSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCD
                     TIVEAAALLPAFDQAVEGNRHQDDYGSGLGIAEEKFHYYLDLNDRYVYFYEPVNVEYF
                     AMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQHVMSLLTPVYVAGQLKG
                     IVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHD
                     LPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMT
                     GLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDNLGHQEGDLAITLHDAY
                     KASDERLYVNKQNKNSRS"
BASE COUNT         4406 a         4175 c         4358 g         4244 t
ORIGIN      
        1 agttaacctg ttgacattca ggtgttttaa aggtaacagc tcatggtgtg ttatccagta
       61 agatggagaa aattaacggt cagcccgcac gctataacca gattgttcag ctaagaaaga
      121 aaaccagagt tgctaatcaa agggcgaaca caacggcatg gttatgttta tttatatagt
      181 tagttttatg gataaaattg aaattataaa tataaataat gaattagcag aagcatcaac
      241 agtgatatct tattctctgg tcgatttatg taaaaccgac cagtattatc gataaattat
      301 cgtctgactt attttgcttc gataaagctt acaccgcagc cacaatctta atttcaactt
      361 tatacttcgg gttcatcaaa cccgcttgta ccgtgcagcg caccggcgca tgacccgcga
      421 caacccaggc atcccacgct ttattcatcg ccgcgaagtc gtttttatcg gccaggaaaa
      481 tggtggcatc cagaatgctc gatttattgc tgccctgttt ttccagcacg gcgtcaatct
      541 gtgccagcgt gttggcggtt tgctcaaagg catcggcgtc gaggttttcc ggtacaccag
      601 tgtagtagag cgtgttgttg tggattacta catcagacca gcgggcttca gcatcgatac
      661 gaacgatagt catcattttg tcctcattat acttccattt tcgtgctgca aagactgcca
      721 caatgtgagg ttgccgtcac cacttcaact ggcgaaagcg ccctcatcct gacataatcc
      781 ctgttcaaat caacaggggg tagtgtgacg gacgattttg caccagacgg tcagctggcg
      841 aaagcgatac caggctttaa gccgcgagaa ccacagcgac agatggcggt agccgtcacc
      901 caggcgatag aaaaaggcca gccgctggtg gtggaagcag gaaccggtac gggcaaaacc
      961 tacgcttacc tggctcctgc gctgcgggcg aaaaagaaag tcattatctc gaccggctca
     1021 aaagcgttgc aggatcagct ctacagccgc gatttgccaa cagtctcaaa ggcattgaaa
     1081 tatacgggca acgtggcgct gctgaaaggg cgctcaaact acctctgcct cgaacgtctc
     1141 gaacagcagg cgctggcggg gggcgatctg ccggtacaaa tcttaagcga tgtgatcctg
     1201 ctgcgctcct ggtctaatca aacagtcgat ggtgatatca gcacctgcgt cagcgtggcg
     1261 gaagattcac aggcgtggcc gctggtcacc agcaccaacg acaactgtct tggcagcgac
     1321 tgcccgatgt ataaagattg ctttgtggtc aaagcacgta aaaaagcgat ggacgccgat
     1381 gtggtggtgg taaaccatca tctctttctg gcggatatgg tggttaaaga gagtggattt
     1441 ggcgagttga tccctgaagc ggacgtcatg atcttcgacg aagcccacca gctaccggac
     1501 attgccagcc agtattttgg tcagtcactc tccagtcgac aactgctcga cctggcaaaa
     1561 gacatcacca tcgcctaccg caccgaatta aaagacaccc agcagttaca aaagtgcgct
     1621 gatcgtcttg cccagagtgc gcaggatttt cgtctgcaac tcggtgagcc aggttatcgc
     1681 ggtaacctgc gtgagctgtt agctaatccg caaattcagc gggcattttt actgctcgat
     1741 gacaccttgg aactttgtta tgacgtggcg aaactgtcac tggggcgttc cgccttgctg
     1801 gatgcggcat ttgagcgcgc cacgttgtat cgcacacggc tgaagcggct aaaagagatc
     1861 aatcagccgg gctacagcta ctggtacgaa tgcacttcgc gccattttac tctggctctc
     1921 acgccgctca gcgtggcgga taaattcaaa gagttaatgg cgcaaaagcc cggtagctgg
     1981 atcttcacct cagcaacgct gtcggtgaac gacgatctgc atcatttcac ctcgcggctt
     2041 gggatagaac aggcggagtc gttgctattg ccaagcccgt ttgattacag ccgccaggcg
     2101 ttactctgtg tgccgcgcaa tctgccgcaa accaaccagc caggttctgc tcgccagtta
     2161 gcggcaatgc tgcgaccgat catcgaagct aacaacggtc gttgttttat gctttgtacc
     2221 tcgcacgcca tgatgcgcga tctggccgag cagttccgcg ctaccatgac gcttcccgtt
     2281 ttgttgcagg gggaaaccag taaaggccaa ctgttgcagc agtttgtcag tgccggtaat
     2341 gcgcttcttg tggcaaccag tagtttctgg gaaggggtgg acgtgcgtgg cgatacattg
     2401 tcattggtaa ttatcgacaa attgccgttt acctcgctgg atgatccact gttaaaagcg
     2461 cgcatggaag attgtcgttt gcgcggtggc gacccgttcg atgaagtgca actaccagat
     2521 gccgtcatta ctctcaaaca gggggtaggt cgactgattc gcgacgccga cgatcgtggc
     2581 gtgctggtga tttgtgacta tcggctggtg atgcgtcctt acggcgcgac gtttctcgcc
     2641 agtctgccgc ccgcgccacg cacccgtgac attgcccgag cggttcgttt ccttgcgata
     2701 ccatcctcca ggtaatttgt tacggagtat ggtatgatgc gcgccttttt attgatctaa
     2761 gcacgaagac ccatgcgaat tctggctatc gataccgcga cagaggcctg ctctgtcgcc
     2821 ctgtggaacg acggtactgt caacgctcat tttgagcttt gccctcgtga acatactcaa
     2881 cgaatcttac cgatggtgca ggatatcctg accaccagcg gaacttccct gactgatatt
     2941 aacgctctgg cttacgggcg cggccccggt agctttaccg gcgtgcgcat tggtattggc
     3001 atcgcgcaag ggctggcgct tggcgcggaa ttaccgatga ttggcgtctc cacgctaatg
     3061 acgatggcgc aaggggcgtg gcgcaaaaac ggcgcaacac gcgtgctgtc agccattgac
     3121 gcgcgaatgg gcgaagttta ctgggccgaa tatcagcgtg atgaaaacgg tatctggcac
     3181 ggtgaagaaa ccgaagccgt actcaaaccc gaactcgttc atgaacgaat gcaacagctt
     3241 tccggcgaat gggtgacggt agggacgggc tggcaagcct ggccggatct cggtaaagag
     3301 agcgggctgg ttttgcgcga tggcgaagtg ttactccccg ctgcagaaga tatgctgccg
     3361 attgcttgtc agatgtttgc cgagggtaaa acggtggcgg tggaacatgc cgagccggtt
     3421 tatttgcgta acaacgtcgc atggaagaaa cttccgggca aagaatgaat cttaagagta
     3481 tgccctgaga aaaaaaggag tcgcatcatg gcggttcaaa agaatgttat caaaggcata
     3541 ctggcaggta cgtttgcgct aatgctgagt ggttgtgtca ctgtgccgga cgccattaaa
     3601 ggcagcagtc ccacgccgca acaagattta gttcgggtga tgagtgcgcc gcagctgtac
     3661 gttggtcagg aggcacgctt tggtggcaaa gtggttgcgg tacaaaacca gcaagggaaa
     3721 acccgcctgg aaattgctac cgtaccgctg gacagcggag ctaggccgac gctgggagaa
     3781 ccttctcgcg ggcgcattta tgccgatgtg aacggttttc tggacccggt ggatttccgt
     3841 ggacaactgg ttacggtagt cgggccaatc accggtgcgg ttgacggcaa aatcggcaat
     3901 acgccctata aatttatggt gatgcaagta acgggttaca aacgttggca tttaacccag
     3961 caggtgatta tgccgcctca gccgattgat ccatggtttt atggcggtcg tggctggccc
     4021 tatggctacg gcggatgggg ctggtataat cccggccccg cgagagtaca aacagttgta
     4081 accgaataat tgcttgtttt taaagaaaaa gaaacagcgg ctggtccgct gtttctgcat
     4141 tcttacggta aagataaaaa taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt
     4201 atcaagctgg tatgattagt taatattatg ttaacagcgt gtatatcatt tggggttgcg
     4261 atgacgacga acacgcattt tagaggtgaa gaattgaaga aggtttggct taaccgttat
     4321 cccgcggacg ttccgacgga gatcaaccct gaccgttatc aatctctggt agatatgttt
     4381 gagcagtcgg tcgcgcgcta cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg
     4441 accttccgca agctggaaga acgcagtcgc gcgtttgccg cttatttgca acaagggttg
     4501 gggctgaaga aaggcgatcg cgttgcgttg atgatgccta acttattgca atatccggtg
     4561 gcgctatttg gcattttgcg tgccgggata atcgtcgtaa acgttaaccc gttgtatacc
     4621 ccgcgtgagc ttgagcatca gcttaacgat agcggcgcat cggcgattgt tatcgtgtct
     4681 aactttgccc acactctgga aaaagtggtt gataaaaccg ccgttcagca cgtaattctg
     4741 acccgtatgg gcgatcagct atctacggca aaaggcacgg tagtcaattt cgttgttaaa
     4801 tacatcaagc gcttggtgcc gaaataccat ctgccagatg ccatttcatt tcgtagcgca
     4861 ctgcataacg gctaccggat gcagtacgtc aaacccgaac tggtgccgga agatttagcc
     4921 ttcctgcaat acaccggcgg caccacaggt gtggcgaaag gcgcgatgct gactcaccgc
     4981 aatatgctgg cgaacctgga acaggtcaac gccacctatg ggccgttgct gcatccaggt
     5041 aaagaactgg tagtaacggc actgccgctg tatcacattt ttgccctgac cattaactgc
     5101 ctgctgttta tcgaactggg tgggcagaac ctgcttatca ctaacccgcg tgatattcca
     5161 gggttggtaa aagagttagc gaaatatccg tttaccgcta tcactggcgt taacaccttg
     5221 ttcaatgcgt tgctgaacaa taaagagttc cagcagctgg atttctccag tctgcatctt
     5281 tccgcaggcg gtgggatgcc tgttcagcaa gtggtggcag aacgctgggt gaaactgacc
     5341 ggacagtatc tgctggaagg ttatggcctg accgagtgcg cgccgctggt gagcgttaac
     5401 ccgtatgata ttgattatca tagcggtagc atcggtttgc cggtgccatc gacggaagcc
     5461 aaactggtgg atgacgacga caacgaagta ccaccgggtc aaccgggtga gctttgtgtc
     5521 aaaggaccgc aggtgatgct gggttactgg cagcgtccgg atgctacaga tgagatcatc
     5581 aaaaatggct ggttacacac cggcgacatc gcggtgatgg atgaagaagg attcctgcgc
     5641 attgtcgatc gtaaaaaaga catgattctg gtttccggtt ttaacgtcta tcccaacgag
     5701 attgaagatg tcgtcatgca gcatcctggc gtacaggaag tcgcggctgt tggcgtacct
     5761 tccggctcca gtggtgaagc ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc
     5821 gaagagtcac tggtgacttt ttgccgccgt cagctcacgg gatacaaagt accgaagctg
     5881 gtggagtttc gtgatgagtt accgaaatct aacgtcggaa aaattttgcg acgagaatta
     5941 cgtgacgaag cgcgcggcaa agtggacaat aaagcctgag cgttaagtca gtcgtcagac
     6001 gccggttaat ccggcgtttt ttttgacgcc cactaaagag aaaacaattt gaattaccaa
     6061 atgattacta cggacgatgc gctggcttct ttgtgtgaag ccgtccgtgc ctttccggcg
     6121 atagccctgg atactgaatt tgttcgtacg cgcacttatt acccgcagct ggggttgatt
     6181 caacttttcg atggcgagca tctggcgcta attgatcctc tcgggatcac cgactggtca
     6241 ccgctgaaag cgatcctgcg cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa
     6301 gatctggaag tgttcctcaa tgtctttggc gaattaccac aacccttgat tgacacgcaa
     6361 atccttgctg ccttctgcgg acgcccgatg tcatggggtt tcgcttccat ggtggaagag
     6421 tattccggcg ttacgctgga caagagtgaa tcgcgcgccg actggctggc cagaccgctg
     6481 accgaacgtc agtgtgaata cgcagcggcg gatgtctggt atctgttacc gatcactgcc
     6541 aagcttatgg tagaaacgga ggcctccggc tggctacctg cggcgctgga tgaatgccgc
     6601 ctgatgcaaa tgcgtcgtca ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc
     6661 aatgcctggc agttacgcac acgccaactg gcctgtctgc aactgttagc cgactggcga
     6721 ctgcgcaagg cgcgagagcg tgatctggcg gtgaactttg tcgtgcgtga agagcatttg
     6781 tggtcggtcg cgcgttatat gccgggaagt ttaggcgaac tggacagcct gggtttatcc
     6841 ggtagcgaaa tccgctttca cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca
     6901 ttgccggaag atgccttacc gcagccgatg tttaacctga tggacatgcc tggttatcgt
     6961 aaagcgttta aagcgattaa gtcgctgatt actgacgtga gcgaaacgca taagatcagc
     7021 gccgaattgc tggcatcgcg tcggcaaatc aaccaactgc tgaactggca ccggaaactg
     7081 aaaccgcaga acaatttgcc ggagctgatt tccggctggc gtggtgagct gatggcggaa
     7141 gcattacaca atttattgca ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg
     7201 cgctcagcgc cacatccggc tttggcaaac tacaaatcca acaccaggcg tttacccttc
     7261 gcacgcgaac aacagatcaa catactttgc tggctggcac gctcttcatc gctaaaatac
     7321 tgatcccgat ggtcagcttc accttccagt attgccgttt cgcaggttcc gcatacccct
     7381 tcacgacata aacattccac tttcgcggct ttattatttt caataacctg caaaatagtc
     7441 atctcttccg gcaccacaaa ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg
     7501 ccggttttgt cttcgatagc aaattgctca aagtgcagcg tatcggcggc gatgtccaga
     7561 cgcgcagctt cacttcttac cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt
     7621 gtgccaggtt cgatatccgc caataatcgc gctaattcca gtcgtgttcc ggttgatgaa
     7681 tgatgcaaat ggactttctc agcctgcgga tgctggacta gctcatcacg aaatgcgcaa
     7741 ctttctggat ttcgcgagca gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct
     7801 gccatgtgcg acaggaaagg ggtgatacca ataccgcccg cgataaacag atgctttctg
     7861 gctgagggaa ttagcgcaaa caggttatta ggcgttgaga tcgttaaccg atcgcccact
     7921 tttacctgct gatgcaaaaa gcgggaaccg ccgcgcgagt tttcttccag tcgaacggca
     7981 atctgataac tagaggtgtt atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg
     8041 ttatcaccat cgctcatctg cactaggatg tgacttcctc cggtgaacgc aggcagcggt
     8101 ttgccatcgg ttgccaccag cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg
     8161 ctcacctgta cttcaaacat ttgatagtct gacattgctg tactccatca aaaatcagcc
     8221 cagcggcgga aaataccgcc gccgatgtca ttagtcctta aacacctgcg ccagcagatt
     8281 atggaaatgg gcgataccat gttcggaaat gccactgccg ctactgtcgg ccatgatgcg
     8341 cccctgacca cgatagccac gcgatttcag ccctttctgt acgctttcaa ccagacgtaa
     8401 atcttccgga tggaacacat cgcgatacca ctcaatcagc gatttttgct cgttggttaa
     8461 ctcttcattg gtgaagtaaa tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa
     8521 ttcataaatg accgtcatca tccctttgat cggggtgacg ttcagcatcg tgcacggcca
     8581 cagccagaaa ccgtggaatg ccgcatccgt accctcttca aatttaaacg actgttcgga
     8641 ctgtttggcg aaaccgtatt gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc
     8701 aacctgtacg gagtcggaga aacctagatg cgccggacca cagtgatagc actcgagata
     8761 gttatcgaca atgttcttcc agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt
     8821 cagattgtgg acttccgggc aggcttccag cactttcgcg cccagaccgg tgtattgatt
     8881 cacttgaagt acgaaaaaaa accgggagga cattggatta ttcgggttct gatgggatta
     8941 gatttggtgg ggcttgcaag cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga
     9001 aagatagtaa aaaattgctt ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat
     9061 catttcaata acagcttcaa aaaacgttca aaaccgataa caaccaggct gtaaccaaat
     9121 gactcatatc acaaatcagc tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg
     9181 tgaagtatgt tttagctggt tcatggttgt tatacggctt tttttacatc ttgtggttct
     9241 tgtgaaggag ctacaagacc ttcctgttca taaataccat actttgacaa aatcttttta
     9301 cgagtatctg ttggtaatga ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata
     9361 ccttgcaaat cagcctggat ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca
     9421 aatcctttct ttctgaacaa ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg
     9481 acaattgcga tcagtttagt tttcaatgtg ctgcggtcag gttgagaatt tttattctct
     9541 tgattatttt tatgctcaac cattggcatg ctctgacttt ggataaacca ctctattgga
     9601 cgccctgttg cgtaggctat ttgtgctaat cgatctaaag acgggtatgt cttgccagac
     9661 aggtagtttc tgataacccc atcagaaagt ccgcacttag ctgcaaacgc ccggcttgat
     9721 tctccattca ttgcctcttc aaggcgttcc ttgaaacgca ttatttccgg ggcataaaaa
     9781 tgcgctctgc atttttgctt tctttttcac ctgtcatgct caatctctct gttatataaa
     9841 gaattttaca gaattctttg atttgcattg gttaaaataa aaatgtgcat agctcttgaa
     9901 atgcgcattt ttctgcgata tcgtttttcc cgatagcggt agccggtatc agttaccgaa
     9961 aacgttaaga gagtaaggat cgcagaatgt cagcaaaaaa ccaagactgg cacgcagagc
    10021 agataaaggc tgctgttcgt atgaagggga agacactatc tcaattatcc agagaggcag
    10081 gcttaaagcc tgacactatg cgtaacgtat ttcgctgcca tataccacga tacgaagcaa
    10141 tcattgctag atatttggat atggaaccag cccagatttg gccttctcgg tatatggaga
    10201 aaacatcatg ttaacccatt gggtaacagc gcaagaatgc gtaggaatgt catgctttcc
    10261 taaacatgag gtgagtattc gccgcaatct tgaaaagctt gcagccaatc atccagaact
    10321 acgtcgcaag cgtacgggtt caaaggtttt ggaatttcat atatcagttc taccagcggc
    10381 agcacgtgtt gagttgttat taaaatctga gcaaatagaa acaagccagg ggtattttga
    10441 aatcgcccgc cccacgctgg aagcgcacga ttacgatcgt gaggcactgt ggagcaaatg
    10501 ggataaagcc agcgattccc agcgcagact tgctgaaaaa tggttgcctg cggttcagac
    10561 tgcagacgaa atgcttcagc cgttattggt gcgattgtcg tgctatttat ctacaggaag
    10621 attaaaagtt aacgcttaaa ttgcacaaag gctgcacaca ggcagccttt gctatttttt
    10681 agaggtgacg ttaccaccat cccagctcag tgccgagtac gacaataatc gccacaccca
    10741 tcataataat cgttgttttt ttcatcttta tgctcccggg gcagcatagc cgccaataaa
    10801 aaaccatgct aaaaatacga ccgctgtaat gaagattaca accggaaaaa taataccgat
    10861 tcgcatgtta tcaccaatca aaaggatgtg aatacgcctc aggaatgtag cgctggatgc
    10921 gcgacaaaag gtgttttctg tttgtgtttt ttctcctgat acatcgcaat atcggcagca
    10981 tgcagggcac tgtctgcatc ggttgtttca ggatcgactt ctactatgcc aagactggca
    11041 ccgggataat acaaatcaac atcacctaag tgatattctc cacgtatttg ctgttgaata
    11101 cgttctcgca gcgacgaaat atccgcattc tcgttgttta gtgaaacaac caaaaactca
    11161 tcaccaccga gacgaccaat aacttcgcca ttttgctgga gcgtattaag gcgctcgcca
    11221 acctgaatga gaaacagatc gccactatta tgaccaaaac gatcattgat taatttgaag
    11281 ttatccagat cgataaacgc gatcattatc ttatggttaa gatgccgggc gagggaaaac
    11341 agcgtcgtca gattttcaaa aatcgcccgc cgattcggta gcccggttaa cgagtcggta
    11401 tacgattgcg caatcagcgc agcattggct tcgtgcagct gttcaaccag tgcctctttt
    11461 tgaatatatt gtgcaatcag tccggcgaat aactgtaaaa cctgttccgc gcgttcactc
    11521 cactggcgct tctcactgct ggcggcgcaa agcgtgccat agaatgatcc atccggtaag
    11581 tgaattggcg tgctcagaaa tgtggtgatg ccaagattgc gtgccgcaat acagtcgccc
    11641 caacggtcag ggacttcatc gctgaaaaag cagttttcat caatggcgcg tttgcataac
    11701 gagtaatccc acgagacggt aaaattctcc gggatgtgca ttttctggct gttacgggca
    11761 aacataatat gctgcaggcg cgcttcgaca tccactttgg tcaggtaggt tgattccata
    11821 tcagtgacca tttccagcat ctccagaagc tgtcggacca gactttccag tgactgttct
    11881 ttggcaaggg attgcgagac gcgggcgata atctgatctg acacggaaca actccaggat
    11941 acggcgggta agtgctgatt atttaatctt tgcaggcatg ataaaacatc atcaacaaag
    12001 atgaaacaag acgtaatatg ttttgagtga atgtaataaa aatagtgctg caaaacaaaa
    12061 cgataccgcg atactctaag cttggataaa tatgtgagat caataaatga aaattatcag
    12121 ttttgttctg ccttgcttgc tggtcctggc tggttgttca accccttctc agccagaagc
    12181 acctaaaccg ccgcagattg gtatggcaaa tccggcgtcg gtctattgcc agcagaaggg
    12241 cgggacgctc attccagtgc agacagcgca aggggtcagc aacaattgca aattaccggg
    12301 cggtgaaacg attgatgaat gggcattgtg gcgacgggac catccggctg gtgaaaaata
    12361 accgccggat gccgtactaa aatcacaagc tacgtagcca gtcggccagc accagcgcat
    12421 ggttctgcgt ggtgtttttt gctgcgtaga gcagggtcag cggctgtttt ttggcgatgt
    12481 ccgccagccg ctttccttct tgctcgtgtt gcgccagttc tgcgcgatat tgctcgcgaa
    12541 aggttgcaaa atcgacgact tcgccgtgaa aggctttgcg cagttccgtt gacggcgtga
    12601 tttctttatc ccactcatca agggctaaat cggttttttt gataccgcgc ggccagaggc
    12661 ggtcgaccag tacgcgataa ccatcgctct gttcagccgg atcataaacg cgtttgcact
    12721 gaatattcat cgtgtttgtt ctccctttca cgcaagattt gtcacgtcac tatagcaccg
    12781 gatattagcg aatttctttg tctctcccgg cgcaaagtcc gaaaatcgcc atcagtagtg
    12841 aaagtattgc aacacccatc agtggtacgg accagttacc gttagcatcg tgtattttac
    12901 ccattagcgg cggcccacag gctgccagca aatacccgac ggattgtgcc atccccgaga
    12961 gtgccgccgc ctgatgcgca gaacttgccc gaagaccaat gaacgtcaac cccagtatca
    13021 ttgttgcgcc ggaaccaaaa ccgaaaagca gagtccaggt gatcgcgtgc gctggcataa
    13081 agcagagccc aaccgcgccc actgcgcaca tcaaggcaac gaacgctgca ataccacgct
    13141 ggtctttcac atgatgtaag aaaagtggga tcagcaaacc gggagctgct gtggctagtt
    13201 gcagcaaacc atgcagtgaa cccgcctgtg cttcgctata gccgtgactg atgaggatcg
    13261 ccggaagcca gccaataatc acgtaataga ccagtgagtt gatcccaaga aacaatgtta
    13321 cctgctaggc aagtggcgaa cgccagatac cccgagtatg taaggcgcgt gaggtactca
    13381 aatttgcatg ttgctgactt cgccactgtg gcagccataa aaaaagagcc agcagaggaa
    13441 aacacatcag catgaacaac gcgccttgcc agccaaaacc gttcaaagcc agcggcacga
    13501 ccatagccga tcccagtgcc gctgcagctc ccattgtcag ggaatatgcg ccggtaagtc
    13561 tggcgacgga atgagggaaa tcgcgtttaa ttaatcctgg cagtaagaca ttgcctaatg
    13621 caatcccacc gccaatgacc gctgtaccgc caaataataa gtaaggcgaa gggagagaac
    13681 gaattgcgat acctgcacag atcaaaagta acgcggcaaa caggctacgt tccataccaa
    13741 aacgtcgcgc caccggggca gccagaggtg agattagcgc aaaggccaat aatggcaggg
    13801 tggttaataa gccggtttgc gctgtcgtca gcgagtaagc ggaacgaatc gtatccagta
    13861 acggcgctgc gccggtaaag gtgacgcgta atgttgtggc aatcatcaga atgccagcga
    13921 taaggacaat cctgtttttg ccgcttaatg aagttgaaca ggtcatatgc ttctcattgt
    13981 tacaaacgag gctgtacctt accgttttta ttgttatgtt gaatcaagct aaaatgacaa
    14041 attatcgcta agttcggaca aaatgatgca caggttgaat ctcaatggct atgaacccga
    14101 ccgacaccat gaagccgcgg tcgcattttg tattcacgcc ggaacggatg agttaaccag
    14161 tcctgtacat cagcaccgca aaggacagct gatactagca ttacatggtg caattacctg
    14221 tacggtggaa aatgctttgt ggatggtgcc gccacaatat gcggtttgga taccgggcgg
    14281 ggtgaagcat agcaatcaag tgacagctaa cgctgaactc tgttttctgt ttatcgaacc
    14341 cagcgcagtg acaatgccga caacatgttg cacattgaaa atatcgccct tatgccggga
    14401 attaatttta accctggcaa acaggacgac gactcagcgt gctgagccga tgacccgcag
    14461 attaattcag gtcctgtttg atgaactccc tcagcagcca caacaacagc tgcacctgcc
    14521 cgtttcttct catcctaaaa tccgcacgat ggtggagatg atggcgaaag ggcccgtcga
    14581 gtgggggtca ttggggcaat gggctggctt ttttgcgatg agtgaacgca atcttgcccg
    14641 actgatagtg aaagagacgg ggttgagttt tcgccagtgg cgtcagcagt tacagctgat
    14701 tatggcgctt cagggattag tgaaaggcga tactgtacag aaagttgctc atacgctggg
    14761 gtatgattca acaacggcct ttattactat gtttaagaaa ggcctagggc aaacgccagg
    14821 ccgatatatt gcgggattaa ctactgtttc cccacaatca gcgaaaccag acccgcggca
    14881 ataagcgggc caaccggtac gccgcgaaac agcgctacgc ctaaaacagt gcccaccagc
    14941 agcccagcaa ccagttgcgg ctggctgccc attaacgtca cgccgcgccc acccagccag
    15001 gagacaataa cgcctactgc aattgccacc agcgatttcc agttcagaaa cgagtgaatc
    15061 aaggttgagg gcggtagcgt cccgctggcg atgggggcca taacgccaat cgtcagaatg
    15121 attatcccga tacttagccc ctgtttttca atccacggga aaaaggtgct taacggtgtg
    15181 acgcggacaa tgatcaacac cagaattgaa acggcgacag tggtgttatg actgataaag
    15241 cccagcgcag ctaacccgag caggatcagc aaagtgacat caaacatcgt ggtttccttg
    15301 ccaaaaaata ataagcctgc ttactttacg cttgctgcag gaaggaagta gaaaatcttt
    15361 acgttttttg cataaaaaat caggcgaact gtcatcaaac tgccatttgt gcacgctaac
    15421 aataacgtga aaacagctat ctcccggggg catgctatga acgatcaaat gttcgtcgag
    15481 acactgatta tcacatcatc gttttttgct attgcggttg tgctggtttt gtccgttctg
    15541 cttattgaac ggataggcta gctacgcagt gcggcggaaa ttgaccagtt ctggttgcgc
    15601 aatgcgcaaa taatccgcgg ttttcaaaat aacggattta tccagcatcc ctgtattgaa
    15661 ggcgatttca tcaaatcgtt cgaagagtag agggtcggca accagtttga gttttggatg
    15721 gaagctgaaa ggggggatcg cgccgaagac acagcctgtg agttcatcaa cttccgccgg
    15781 gctggccaac gaagcgcgta atccgcctat atggctggcg agttggctca gatcggcctg
    15841 ttgatcggct gcgaggatcg ccagaacatg ttgatttacg ccattgcctt tgactttaca
    15901 taccagtgcc ttagcaccct ggcctagagc ggtgccacga atttctgaca ccgcttcaca
    15961 tttacctacc gcttcatggg taacgacgcg gaagttagca ccttcttgtg ataataaagc
    16021 gattaactgc tgatgcgtca cgcttccttt agccatttca gtcatttcac gctcctgaga
    16081 ttacaagcaa acaaccacag aaggttatca tgaacggctg tttttgttct gcttattgac
    16141 atataaacgc tcatcggaag ctttatacgc atcatgtaac gtaatcgcta aatccccctc
    16201 ctgatgaccg aggttgtcat ttatttgctt taacttgtcc atgtcaattg caataaacat
    16261 caccgaagaa ccggattgca ccagtttctg caaccgctgc tccagttcag gggttaaaat
    16321 tttgcgatta tacagtccag tcatcgcatc actaatattt tctcgcgaca cattttggta
    16381 taaacggaag tgcatccgga ccatattcag caaaattaac gccgtcaaaa tccagaacag
    16441 aacgcttttc cacgaagacg tgataaagta aagaatatca atggataacg agacacgaat
    16501 gccgcccggt aagtcatgga cgtaactgac atactggaac agattatctt cgctctggtt
    16561 gatgataatg tcgcgccccg aatcggtatc ggttagcgtg acattgagaa aacgccagag
    16621 gagagggcgg tcatgagtgt aaaagatatt ccgcaaattg tttttgttaa tatccagcag
    16681 cacaatccct tttagctgcc ctgcgacata taccggcgtc agcaaactca tcacgtgctg
    16741 tttagtacgc tgatcctggt aaatgctcga caatacggta cgcccggtaa ataccttttc
    16801 aatatctttg cgatcgatgc caatacttcc tgactgcagg aaggaccagt tattcatcgc
    16861 aaagtattca acattaaccg gctcataaaa atagacatag cggtcattga gatcgagata
    16921 atagtgaaat ttctcttcgg ctatcccaag acctgaaccg taatcatcct ggtggcggtt
    16981 gccttccact gcctgatcaa aagcgggtaa tagtgctgca gcttccacaa ttgtgtcgca
    17041 gtcagtggat gcggtttgta gtgtgccgcg taagcccggg tatttatggg cagtcaggtt
    17101 taagccatgg gtattattgg cagactcaaa agcctcgcag atggcgcgcc gctgttctgg
    17161 cgacacttcc gactgctcag cag
//
LOCUS       DADMEQ010000081        17099 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.082, whole genome shotgun sequence.
ACCESSION   DADMEQ010000081 DADMEQ010000000
VERSION     DADMEQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17099
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.082"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(464..1660)
                     /gene="hemY"
                     /locus_tag="JNZ05_002707"
     CDS             complement(464..1660)
                     /gene="hemY"
                     /locus_tag="JNZ05_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HAY8565885.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1663..2874)
                     /gene="hemX"
                     /locus_tag="JNZ05_002708"
     CDS             complement(1663..2874)
                     /gene="hemX"
                     /locus_tag="JNZ05_002708"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY8565886.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPVPQ
                     GDTPAAAPQGE"
     gene            complement(2896..3636)
                     /gene="hemD"
                     /locus_tag="JNZ05_002709"
     CDS             complement(2896..3636)
                     /gene="hemD"
                     /locus_tag="JNZ05_002709"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HAY8565887.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFTIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3633..4574)
                     /gene="hemC"
                     /locus_tag="JNZ05_002710"
     CDS             complement(3633..4574)
                     /gene="hemC"
                     /locus_tag="JNZ05_002710"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAY8565888.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            4961..7507
                     /gene="cyaA"
                     /locus_tag="JNZ05_002711"
     CDS             4961..7507
                     /gene="cyaA"
                     /locus_tag="JNZ05_002711"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HAY8565889.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7547..7867)
                     /gene="cyaY"
                     /locus_tag="JNZ05_002712"
     CDS             complement(7547..7867)
                     /gene="cyaY"
                     /locus_tag="JNZ05_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HAY8565890.1"
                     /translation="MNDSEFHHLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7915..9228)
                     /locus_tag="JNZ05_002713"
     CDS             complement(7915..9228)
                     /locus_tag="JNZ05_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HAY8565891.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9225..9530)
                     /locus_tag="JNZ05_002714"
     CDS             complement(9225..9530)
                     /locus_tag="JNZ05_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000463015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565892.1"
                     /translation="MFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFD
                     GAQLWQYRTADPKQVRLIWKEQHSGYVYGVNYKTGYLDGKITDDGEFIPAPRNEVTK"
     gene            complement(9552..10703)
                     /locus_tag="JNZ05_002715"
                     /pseudo
     CDS             complement(9552..10703)
                     /locus_tag="JNZ05_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10892..11095
                     /locus_tag="JNZ05_002716"
     CDS             10892..11095
                     /locus_tag="JNZ05_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY8565893.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11132..11956
                     /gene="dapF"
                     /locus_tag="JNZ05_002717"
     CDS             11132..11956
                     /gene="dapF"
                     /locus_tag="JNZ05_002717"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAY8565894.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            11953..12660
                     /locus_tag="JNZ05_002718"
     CDS             11953..12660
                     /locus_tag="JNZ05_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HAY8565895.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12657..13553
                     /gene="xerC"
                     /locus_tag="JNZ05_002719"
     CDS             12657..13553
                     /gene="xerC"
                     /locus_tag="JNZ05_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAY8565896.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13553..14269
                     /gene="yigB"
                     /locus_tag="JNZ05_002720"
     CDS             13553..14269
                     /gene="yigB"
                     /locus_tag="JNZ05_002720"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HAY8565897.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14353..16515
                     /gene="uvrD"
                     /locus_tag="JNZ05_002721"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14353..16515
                     /gene="uvrD"
                     /locus_tag="JNZ05_002721"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HAY8565898.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACSELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(16662..17054)
                     /locus_tag="JNZ05_002722"
     CDS             complement(16662..17054)
                     /locus_tag="JNZ05_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565899.1"
                     /translation="MRPGGVFYVAGDKVGIVRLDAFKASKDIQFAVQSGPMLMENGVI
                     NPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVEQLLYLGGT
                     ISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         3959 a         4428 c         4541 g         4171 t
ORIGIN      
        1 ttgatgctgt ttttagtttt aacggcaatt aatatatgtg ttattaattg aatgaatttt
       61 atcattcata ataagtatgt gtaggatcaa gctcaggtta aatattcact caggaagtta
      121 ttactcagga agcaaagagg attacagaat tatctcataa caagtgttaa gggatgttat
      181 ttcccgattc tctgtggcat aataaacgag tagatgctca ttccatctct tatgttcgcc
      241 ttagtgcctc ataaactccg gaatgacgca gagccgttta cggtgcttat cgtccactga
      301 cagatgtcgc ttatgcctca tcagacacca tggacacaac gttgagtgaa gcacccactt
      361 gttgtcatac agacctgttt taacgcctgc tccgtaataa gagcaggcgt ttttttatgc
      421 atcagaaagg tcccggaggt gctttcctcc gggtgagaag gaactactgt ggcgggttat
      481 tctgcaacgt taacatcaaa ccgtcgcgac gcatcgctgc agcttcttcc ggcttgtgca
      541 gtctgtccag cgcgtcggca agccatgcgt aatcgtaggc gtccggacgt tgtttcagcg
      601 ccgcgcggaa ggcgagcgat gcttcctgcc attcaccgtg cttcatcagt gactgaccca
      661 atgtgctcca caacagcggg cgatcaccga cgtttttgat ttgctggcgt agcacttttt
      721 ccagctgttc cgggttattg gttttcagac gcgggatcgg cagcagtagg cgatcgtcat
      781 actggcgttt caggccgtcg atgataattt gctgggcagt atcatgatcg tcacattcaa
      841 taagatgttc cgccattgcc acctgcaacg ctacctgatg acgcgttttc cggctttggt
      901 ttttccacca gttacgcaaa ccttcactac cgttatcggc acgcgcctga tccatcaggc
      961 caatccatgc ctgttgttcc agcattgcac gatgttcttc atcaccaaca tgggctttcg
     1021 ccattgatgg gatgatatcc agcagagaac tccatgcccc tgtgcggata tacgcctgtt
     1081 ccgccagacg taatacttca ggatggcgtg gcgtaacttc cagcagctta tccacgccgt
     1141 ggcgtgcagc atggttttca ttacgggcca gttgcagacg tacacgggtg atttctaccg
     1201 gaatggtgtc attgccggcc agctccgctg cgcgttccag atgttggttg gcgcgtgctt
     1261 catcaccacg ttgttgcgcg gcttcggcag ccagtagata gttcaccacc ggttgttcag
     1321 cgtgatcggc atttttcgcc atcagctttt caacttgctg ataatcacct tccgccagtt
     1381 tcagcagcgc ctgttcggtc tgcttacgcg cacggcgacg cttacgtccg acaaaccatc
     1441 cacgggtgtg cgcgccagtg cggaagatcc gccgcagtag ccactcaatg gcaaacagca
     1501 ctaccatcgc cagaatcaat atgatcgcca ggcccgtgac gctggtttcg atattgtagt
     1561 tgtcggtctg gatcagcaca taaccctgat gaccggcaat catcgggcca accactatcc
     1621 ccgcaatcag caaaacaaag agtaataaca cttttagcat ggttattctc cttgcggcgc
     1681 ggctgccgga gtatcacctt gcggtacagg tgcaggtgca ggtgcaggtg caggtgcagg
     1741 tgcaggttta gcttccgttg tccccgctgc cggttgtgcc agcaggttac gcacgcgagt
     1801 ctgcatcagt ttttccagca tcgcctggct ttgcagggtt tccggaagat ccatcgagat
     1861 attttgctgg cttaactggt ccacctcgtc gaggaacgct ttggtggtgg catcatcagt
     1921 atcgtagtaa gcacgtaccc aggtggagac gttctccagc gcctggcgat aagtctcttc
     1981 ctgatgacgc ggtacagctt gtgctgcgac cagcaggcga gagcgaatat tttcgcgcag
     2041 atagatatcc tgatttggcg ctaacagcgg tacggcggtg tcatcacgac ggcgaatcgt
     2101 aatgaagttg tccataaagt tctgccagct tttttgcaga ttgatacgcc attcgctgat
     2161 ggaactggaa agctcttcac cgtctgaatc catcggcgaa ccatcgctgt cattatcggc
     2221 cagacgcagg ttatctacct gatttgaaag ctgattaagc ttaaggatga tgccgtcata
     2281 atccacctgc gatactgcag aaaggctggc gatatcatcg gtaattgccc gacgaacggt
     2341 aatcagactc gggtcattca tatccgccag gctggcgtct gcacttttca gcaacgctgc
     2401 agaggtcgtg acgtcctgat cgctccacag cttccgtccg gcgagtttca ccagaaaatc
     2461 ggcctgagcc agcagccagg ttttagcatc gctgccggaa atggtggcga tcttttgttg
     2521 gacttcatcc aactgttttg ccagcgtttc ttgctgacga ttcgcctgct taagctgcgc
     2581 agcttgttgc ttaataatgc cttccagctc ggctttttgg ctctcctggg ctttttgcaa
     2641 tgccgtcagt tggttagcca gggcatcgct ggtggcggtc tgattgacgg cctgttgttt
     2701 accccagcca tacaaaccga cacccgccgc cagagcaata gcgatagcca ccgcgctgag
     2761 aatcaatgca gtattgttct tactcttttt ttctgttgcg acaggttgtg acgtggtgtc
     2821 cacggcctcc ctggtctctt caaccacggc ggaggttttt tcttgttccg tcattatggc
     2881 ttcctgttat gagagttatt gtaatgcccg taaaagcgca tcgttgtcag cgttatcggc
     2941 gaccttaatg tcttgccagc ccagttcccg ggcgagtttc gccaaacgct cactgacgac
     3001 caatagtcga cagtgtagta accagtgctc acgataccat tgtgggatca gcgaccagag
     3061 ttgctgcaac atttcaccgc tggtaacaac gaccatcgtc acctcgcggg attgccagcg
     3121 catcgcttct tctgcaccat cgtaatggat tgcgcatcgt tgataacatt cacaaaaagt
     3181 gacctcagca ccgcgcaccg tcagggtatc cccaattagc tcacgaccgc cattgccacg
     3241 taatatcagc gcacgtttgc ccgcaatatt ttgtaattca ggtaattgta gcaagacttc
     3301 gctgatttcc cgatcctgtg ggtagagaat cttctgtccg cttacggtat gtagcgccag
     3361 tgcggtggtg cgtccaatgg tgaaataagt aggtagttgg ggccatttac gatcttgctg
     3421 atgcagctgt gattgggcaa aagcaaccgc gtgttgcgag agggcaaaca acagatcgct
     3481 ctcccccagc gctgccagtt gatcagcaag ttgcggtaat tgtcgacccg gagaaaactc
     3541 aatcagcgga aaatgccagg ccacctgccc cagtgtgcgc agacggctca ctaactcttc
     3601 tccagcggga gacgggcggg tgaccaggat actcatgccg gggcctctcc gttatagact
     3661 tcagcgagga tctcgcgcgc gccgttattc agtagctctt ctgccagcga aatccccatt
     3721 tgttcggcat cttgcggcgc accgcggcgt tcaccgcgaa taatctgcga accgtccggc
     3781 gcgccgacca gcgcacatag ccagatttcg ccatcaataa gctcggcgta gctaccaatt
     3841 ggcacctgac atccgccttc gagacgggta ttcatggcgc gttctgcggt aacgcgcagt
     3901 gcagtttcgt ggtgattcag cgcggcaagc agctcgcgag tgcgtgtatc atcaaggcgg
     3961 cattcaatac cgactgcacc ttgtcctacc gccggaagag aaatctcggg tggtaacgcg
     4021 gcgcgaatgc gggactccag acctaaacgt tttagtccgg ctacggcaag aatgatggca
     4081 tcgtattcgc cgttatctag tttgctcagg cgagtgccga cgttgccgcg cagggagcgg
     4141 ataatcagat ccgggcggcg ttcagccagt tggcactggc gacgtaaact ggacgtcccg
     4201 acgatactgc ctgctggtaa cgcatccaga ctgtcatagt tattggacac aaaggcatcg
     4261 cgaggatctt cacgctcgca aatagtgacc agccccagac cttgcgggaa ttcaaccggc
     4321 acatctttca ttgaatgtac ggcgatatca gcgcgattct ccaggagcgc gacttccagc
     4381 tcttttacaa ataagccttt tccgcctact ttcgccagcg gcgtatcaag aatcacatcg
     4441 ccgcgcgtca ccatcggtac cagttcaacg accaggcccg gatggctcgc catcaacttg
     4501 tctttgacat agtgtgcctg ccagagtgca agtgggcttt ggcgtgtggc aattcttaaa
     4561 acattgtcta acatgcttgt taccgtcatt atcatccgtg gtccatccta acatccttgc
     4621 cagagtgatg tcagtgttgt ggtgaaacgt agacgcctgc gcaaaccgta aaatgaggtc
     4681 tggcagtgga tcctgacagg cgtttcacgc cgtcgtaata aggaatttac agagaataaa
     4741 cggtgctaca cttgtatgta gcgcatcttt ctttacggtc aatcagcaag gtgttaaatt
     4801 gatcacgttt tagaccattt tttcgtcgtg aaactaaaaa aaccaggcgc gaaaagtggt
     4861 aacggttacc tttgacatac gaaatatccc gaatgccgcg tgttaccgtt gatgttggcg
     4921 gaatcacagt catgacgggt agcaaatcag gcgatacgtc ttgtacctct atattgagac
     4981 tctgaaacag agactggatg ccataaatca attgcgtgtg gatcgcgcgc ttgctgctat
     5041 ggggcctgca ttccaacagg tctacagtct actgccgaca ttgttgcact atcaccatcc
     5101 gctaatgccg ggttaccttg atggtaacgt tcccaaaggc atttgccttt acacgcctga
     5161 tgaaactcaa cgccactacc tgaacgagct tgaactgtat cgtggaatgt cagtacagga
     5221 tccaccgaaa ggtgagcttc caattactgg tgtatacacc atgggcagca cctcgtccgt
     5281 agggcaaagt tgttcctctg acctggatat ctgggtctgt catcaatcct ggctcgatag
     5341 cgaagagcgc cagttactac aacgtaaatg cagcctgctg gaaagctggg ccgcctcgct
     5401 gggtgtggaa gtcagcttct tcctgattga tgaaaaccgc ttccgtcata atgaaagcgg
     5461 cagcctgggg ggcgaagatt gtggctccac ccagcatata ctgctgcttg acgaatttta
     5521 tcgtactgcc gtgcgtctcg ccggtaagcg tattctgtgg aatatggtgc cgtgcgacga
     5581 agaagagcat tacgacgact atgtgatgac gctttacgcg cagggcgtgc tgacgccaaa
     5641 tgaatggctg gatctcggtg gcttaagctc gctttctgct gaagagtact ttggtgccag
     5701 cctttggcag ctctacaaga gtatcgattc cccatacaaa gcggtgctga aaacactgct
     5761 gctggaagcc tattcctggg aatacccgaa cccacgtctg ctggcgaaag atatcaaaca
     5821 gcgtttgcac gacggcgaga ttgtatcgtt tggcctcgat ccatactgca tgatgctgga
     5881 gcgtgttact gaatacctga cggcgattga agatttcacc cgtctggatt tagtacgtcg
     5941 ctgcttctat ttaaaagtgt gcgaaaaact cagccgtgaa cgcgcctgtg taggctggcg
     6001 tcgcgcagtg ttgagccagt tagtgagcga gtggggttgg gacgaagctc gtctggcaat
     6061 gctcgataac cgtgctaact ggaagattga tcaggtgcgt gaggcgcaca acgagctgct
     6121 cgacgcgatg atgcagagct accgtaatct gatccgcttt gcgcgtcgca ataaccttag
     6181 cgtctccgcc agtccgcagg atatcggcgt actgacgcgt aaactgtatg ccgcgtttga
     6241 agcattgcca ggtaaagtga cgctggtaaa cccgcagatt tcacccgatc tctcggaacc
     6301 gaatctgacc tttatttatg tgccgccggg ccgggctaac cgttcaggtt ggtatctgta
     6361 taaccgcgcg ccaaatattg agtcgatcat cagccatcag ccgctggaat ataaccgtta
     6421 cctgaataaa ctggtggcgt gggcatggtt taacggcctg ctgacctcgc gcacccgttt
     6481 gtatattaaa ggtaacggca ttgtcgattt gcctaagttg caggagatgg tcgccgacgt
     6541 gtcgcaccat ttcccgctgc gcttacctgc accgacaccg aaggcgctct acagcccgtg
     6601 tgagattcgc catctggcga ttatcgttaa cctggaatat gacccgacag ctgcgttccg
     6661 caatcaggtg gtgcattttg atttccgtaa gctggatgtc ttcagctttg gcgagaatca
     6721 aaattgcctg gtaggtagcg ttgacctgct gtaccgcaac tcgtggaacg aagtgcgtac
     6781 gctgcacttc aacggcgagc aatcgatgat cgaagccctg aaaactattc tcggcaaaat
     6841 gcatcaggac gccgcaccgc cagatagcgt ggaagtcttc tgttatagcc agcatctgcg
     6901 cggcttaatc cgtactcgcg tgcagcaact ggtttctgag tgtattgaac tgcgcctttc
     6961 cagcacccgc caggaaaccg ggcgtttcaa ggcgctgcgc gtttctggtc aaacctgggg
     7021 cttgttcttc gaacgcctga atgtatcggt acagaagctg gagaacgcca tcgagtttta
     7081 tggcgctatt tcgcataaca aactgcacgg cttgtcagtg caggttgaaa ccaatcacgt
     7141 caaattaccg gcggtggtgg acggctttgc cagcgaaggg atcatccagt tctttttcga
     7201 agaaacgcaa gacgagaatg gctttaatat ctacattctc gacgaaagca accgggttga
     7261 ggtgtatcac cactgcgaag gcagcaaaga ggagctggta cgtgacgtca gtcgcttcta
     7321 ctcgtcatcg cacgaccgct tcacctacgg ctcaagcttc atcaacttca acctgccgca
     7381 gttctatcag attgtgaagg ttgatggtcg cgaacaggtg attccgttcc gaacaaaatc
     7441 gattggcaac ctgccgcccg ccaatcagga tcacgatacg ccgctattac agcagtattt
     7501 ttcgtgatga gcgtgccgga aagcgaggct tatccggcat gcaatcttag cggaaactga
     7561 ctgtttcacc cgcctgttgc gtcgccgcct gttccagcaa atcccagaag gtttcgccgc
     7621 tgcgatcgca aatccattca tcgcctttca ggtcaaaatg gtagccgccc tgtttggttg
     7681 ccagccacac ctggtgcagc ggctcctggc ggttaataat gattttgctg ccgttttcaa
     7741 aggtaatggt cagtacgccg ccgttgatct cgcagtcgat atcgctgtcg ccatccctgt
     7801 cgtccagacg ctcttcaatg gtcagccaca gttgatcagc caggtgatga aattcactgt
     7861 cgttcattgt tgtaccctgt ttttaagtga tggcggcagt atagcggcat ggggtcaggg
     7921 cttcaaagtt tgcacctctg ctgctgcgtt ccggcatgat tcatccgtca ccggaataat
     7981 gatgtctctg ttttgaactt cacgtacgtt ctcctctctg acacgagttt tcgccgggca
     8041 gggggagggc gtaatcctta cctctgtatc ggtccatgta taaacattgc gatcgtcata
     8101 agcgaagcga tcgtttttag ttagcggacc gttaactgga ataatttgca agctctttga
     8161 ccgtaattgg cggataaagt aagcatgctg tttgccgcta ttaaatgagc gatcaatgac
     8221 caagtctggc gtatccagcg ggattatctc aagctggtct tcaccaaatg tcgataattt
     8281 tgttacatac agccgatctt tatattgcgc aatattgcca gtaatcagat ggaattgttc
     8341 aggttcaata cctggtaggt ctatccactt gctccactga cggtcgggcg tgagctgttt
     8401 acgccagcga acgcgacttt cccctacttc aaaagagacg tcatcatcta cgggtatcgc
     8461 tttgcctgat aattgaccaa agggataacg atgcagaccc ttattgtcgc gaaaaaccag
     8521 cagtgaatga ggtatccagc gaattatctc aaatgagtct gcattcgccg taaggtgaac
     8581 gccatttaaa taaatattct gcccactgcg gataagcaga tcattggaat atgaatttct
     8641 gtttacactc ataagacgtg gattaatacc ccaccatctt ttttctaata cagtaacatc
     8701 actgctgctc ccctgacgtc gaccggacaa gtagagattt ttactatcca ccagcgtggt
     8761 agagttacct tcgatagctt ttagtgtcgc cagatcaaca cgtgacgcgc cgctattact
     8821 ttcggtgcgc tgtccgtcaa aatagaggct atatttatcg accgcgaagc ggcccatagc
     8881 ctgaaagctg gcaatatcga cagtgggtgt tcccggtgga ttgctaacga ttttgccacg
     8941 ccagagaata acgcggccgt ctgtcaccca gtggtattcc tcattttcta tgcttatttc
     9001 atcggaaggt atataacccg ttttcccaga gatatttttc acaaggcgca aaccggccct
     9061 gctcatctcc ggaaactggg caatgatttt cttatccccc caataacctt taccgtaaga
     9121 cataacaccg tgatcattgt aatagtcgaa ccctatggcc tgtacagcag gcgtcagaaa
     9181 tgtgtgtccc aacaggatga gtgcaactgt gattatttga cccttcattt tgtcacctca
     9241 ttgcgcgggg caggaataaa ttcgccgtca tcagtaattt ttccgtccag atatcccgtt
     9301 ttgtaattta cgccatagac atatcccgaa tgttgttcct tccagattaa gcgaacttgt
     9361 ttcggatcag cggtgcggta ttgccagagc tgcgcgccat caaagctgta aatgtagcgg
     9421 ttatcgcggg cataccatct gcgcaaaaag ggatggtcat taagatgggc ttcgcgttca
     9481 tccggagaac ggttatccag taagatcagc ggtccgtcag attcaaacac gtgtaaaccg
     9541 gaatggtatg gctacccttc ggcacgcgta agtaaatagc cgtagcgttt cccggcgttg
     9601 aaacgtttgt tgataataag attgggatca tcaagtttca cgatctccag cttacggtca
     9661 ccagactctg tttcgatgat ggtataaagc gagtcctgat actgggcgac agcatcactg
     9721 atgggttgaa actgctccgg atcgagtccg ggtaattctt cctgtttaca agcaggtcct
     9781 ttacgccaga acactttctt ttccagcaga gaaaaatcca gacagtgctt tgttaaatct
     9841 tcatcccacg ccagattttc cttgtcgagg actttacgct gcagcccgtt tttatcacgc
     9901 caggtcaaga gtgagccggg catccagcgc acgacggaaa aggtgtccgg gtcggcatca
     9961 agctgttcgc cgttgagcag gattttggtc cgtgtacgag ccagggtatc ccaggggcca
    10021 aatggtacgg caatgcaggg gttaaacgtg gtggaaaact ttcctcgctg atcccaggac
    10081 ttctgcgcca gcacacggaa actctcgggg tcagcgaggc gatgcccatt gatatacaag
    10141 aaattagcgt cacgtaatat cagccccagt aagtcaggat cccagggagg ccggaattcc
    10201 acctgatgca gcgttttgat atccaagcta tttccaccac cattgtcatc ggtgcgcttg
    10261 ccttcaaaat agagactgtg tttatctgcg gcaaaatcac cccatgcctt gaacgaggcg
    10321 acatcgacgg gaggtgtgcc gggagggttt tgcacaactt cacccgcgta gaggatattg
    10381 cgaccatcgc tatgccatga ataatgagga tactaggcct gtgcgccctc atagaggtag
    10441 tcggttatca aatcagtcag aggcggttct ttatcccatt cgtctagtgt tgtcgggtct
    10501 attaataaat tgggtaaccg acgcaaactc ttgtaattca cgtcaggtag agaaagattt
    10561 gtttctttat aagggcgcag gtacaccacg ccttttccct gtttctggta aatattatgt
    10621 tcaggtgcgg aaagccgaca tccccatgcc gacgataaaa acaatgaaca agccaacaaa
    10681 caaaccacat tgcgatagtg cataaagcca tcctggcgcg aggtgttgat cacgaaactg
    10741 ccagcagaac ataaatcctc acgagtaagc gttatactcg cagcatttcc tcacttttca
    10801 gacttcataa agagtcgcta aacgcttgct tttacgcctt ctcctgcgat gatagaaagc
    10861 agaaagcgat gaactttaca ggcaatccat aatgaaaaac gtgtttaagg cactcactgt
    10921 attacttact ctcttcagcc tgacgggctg cggtctgaaa ggtccgctct atttcccgcc
    10981 tgcagataaa aacgcaccgc cgccgaccaa accggtagag acgcaaacgc aatccacggt
    11041 gccggataaa aacgatcgcg ccactggcga tggtccatcc caggtgaatt actaaaagtc
    11101 agtttctgta cccgcgtgat tggagtaaat gatgcagttc tcgaaaatgc atggccttgg
    11161 caacgatttt atggtcgtcg acgcggtaac gcagaatgtc tttttttcac cggagctgat
    11221 tcgtcgcctg gcggatcggc acctgggggt agggtttgac cagctgctgg tggttgagcc
    11281 gccgtatgat cctgaactgg attttcacta tcgcattttc aatgctgatg gcagtgaagt
    11341 ggcgcagtgc ggtaacggtg cgcgctgctt tgcccgtttt gtgcgtctga aaggactgac
    11401 caataagcgt gatatccgcg tcagcaccgc caacgggcgg atggttctga ccgtcaccga
    11461 tgatgatctg gtccgcgtaa atatgggcga acccaacttc gaaccttccg ccgtgccgtt
    11521 tcgcgctaac aaagcggaaa agacctatat tatgcgcgcc gccgagcaga caatcttatg
    11581 cggcgtggtg tcgatgggaa atccgcattg cgtgattcag gtcgatgatg tcgataccgc
    11641 ggcggtagaa acgcttggtc ctgttctgga aagtcacgag cgttttccgg agcgcgccaa
    11701 tatcggtttt atgcaagtgg ttaagcgcga gcatattcgt ttacgcgttt atgagcgtgg
    11761 ggcaggagaa acccaggcct gcggcagcgg cgcgtgtgcg gcggtggcgg tagggattca
    11821 gcaaggtttg ctggacgaag aagtacgcgt ggaactcccc ggcggtcgtc ttgatatcgc
    11881 ctggaaaggt ccgggtcacc cgttatatat gactggcccg gcggtacatg tctacgacgg
    11941 atttattcat ctatgaagca accaggggaa gaactgcagg aaacactcac ggagcttgat
    12001 gaccgggcgg ttgtcgatta tctgattaaa aatcctgagt tttttatccg caatgcgcgc
    12061 gcagtagaag cgatacgtgt gccgcatccg gtacgcggca ccgtttcgtt ggtcgagtgg
    12121 cacatggccc gcgcacgtaa tcacattcat gttctggaag agaacatggc gttgttgatg
    12181 gagcaggcta tcgccaacga agggctgttt tatcgcctac tctacctgca gcgcagtctc
    12241 acggccgcca gcagtctcga cgatatgctg atgcgctttc accgctgggc gcgcgatctc
    12301 ggcctggctg gtgcgagtct gcgcctgttt ccagatcgct ggcgcttagg tgcgccgtcg
    12361 aaccacactc atctggcatt aagccgtcag tctttcgaac cgctgcgtat tcagcgtttg
    12421 gggcaggaac agcactatct tgggccgctt aacggaccgg agctgctggt ggtgctaccg
    12481 gaagcgaaag cggtgggatc ggtggcgatg tcgatgctgg gaagcgatgc tgatttgggt
    12541 gttgtgctgt ttaccagtcg cgatgccagt cactatcaac aagggcaggg aacgcagtta
    12601 cttcatgaaa ttgcgctgat gttgccggag cttctggagc gttggattga acgcgtatga
    12661 ccgatttaca caccgatgta gaacgctacc tacgttatct gagcgtggag cgtcagctta
    12721 gcccgataac cctacttaac taccagcgtc agcttgaggc gatcatcaat tttgccagcg
    12781 aaaacggcct gcaaagctgg caacaatgcg atgcagcgat ggtacgcaat tttgctgtac
    12841 gcagtcgccg taaagggctg ggagcagcaa gtctggcgtt acggctttct gcgctacgta
    12901 gcttttttga ctggctggtc agccagaacg aactcaaagc taacccggcg aaaggagttt
    12961 cggcaccgaa agcgccgcgt catctgccga aaaatatcga cgtcgacgat atgaatcggc
    13021 tgctggatat tgatatcaac gatcccctcg ctgtacgcga ccgtgcaatg ctggaagtga
    13081 tgtacggcgc gggtctgcgt ctttccgagc tggtggggct ggatatcaaa cacctcgacc
    13141 tggagtccgg cgaagtgtgg gtgatgggga aaggcagcaa agagcgccgc ctgccgattg
    13201 gtcgcaacgc tgtggcgtgg attgagcact ggcttgattt gcgcgacctg tttggtagcg
    13261 aagacgacgc gctttttctg tcgaaactgg gcaagcgtat ctccgcgcgt aatgtgcaga
    13321 aacgctttgc cgaatggggc ataaaacaag ggctgaataa tcacgttcac ccacataaat
    13381 tacgtcactc gttcgctacg catatgctgg agtcgagcgg cgatcttcgt ggtgtgcagg
    13441 agctactggg tcatgccaac ctctccacca cgcaaatcta tactcatctt gattttcaac
    13501 accttgcctc ggtgtacgat gcggcgcatc cacgcgccaa acgggggaaa taatgcgttt
    13561 ttaccggcct ttggggcgca tctcggcgct cacctttgac ctggatgata ccctttacga
    13621 taaccgtccg gtgattttgc gcaccgagcg agaggcgctt acctttgtgc aaaattatca
    13681 tccggcgctg cgcagcttcc agaatgaaga tctgcaacgc ctgcgccagg cggtacggga
    13741 agcggaaccc gagatttatc acgacgtgac gcgttggcgt tttcgttcga ttgaacaagc
    13801 gatgctcgac gccgggctga gtgccgaaga agccagtgca ggcgcacacg cagcaatgat
    13861 taactttgcc aaatggcgca gccgaatcga cgtcccgcag caaactcacg acaccttaaa
    13921 acagctggcg aagaaatggc cgctggtggc gatcaccaac ggtaacgccc agccggaact
    13981 gtttggtttg ggggattatt ttgagtttgt gctgcgcgct ggcccgcacg ggcgctcaaa
    14041 accgttcagc gatatgtact ttttggctgc ggaaaaactc aacgtgccga tcggcgagat
    14101 cttacatgtt ggggacgatc tcaccactga cgtgggtggg gcaattcgca gcggaatgca
    14161 ggcttgttgg atcagaccgg aaaatggcga tctgatgcaa acctgggaca gccgtttact
    14221 gccacatctg gaaatttccc ggttggcatc tctgacctcg ctgatataat cagcaaatct
    14281 gtatatatac ccagcttttt ggcggagggc gttgcgcttc tccgcccaac ctatttttac
    14341 gcggcggtgc caatggacgt ttcttacctg ctcgacagcc ttaatgacaa acaacgcgaa
    14401 gcggtggccg cgccacgcag caaccttctg gtgctggcgg gcgcgggcag tggtaagacg
    14461 cgcgtactgg tgcatcgtat cgcctggctg atgagcgtgg aaaactgctc gccatactcg
    14521 attatggctg tgacgtttac caacaaagcg gcggcggaga tgcgtcatcg tatcggacaa
    14581 ctgatgggca ccagccaggg cggcatgtgg gtaggcacct tccacgggct ggcgcaccgc
    14641 ctgctgcgtg cgcaccatat ggacgccaat ctgccgcagg atttccagat cctcgatagc
    14701 gaagaccagc tacgcctgct taagcgtctg atcaaagcca tgaacctcga cgagaagcag
    14761 tggccgccgc gccaggcaat gtggtacatc aacagccaga aagatgaagg cctgcgtccg
    14821 catcatattc aaagctacgg taatccggtg gagcagacct ggcagaaggt gtatcaggcg
    14881 tatcaggaag cgtgcgatcg cgcgggcctg gtggacttcg ccgagctgct gctgcgcgct
    14941 cacgaactgt ggcttaacaa gccgcatatc ctacaacact accgcgaacg ttttaccaat
    15001 atcctggtgg acgaattcca ggataccaac aacattcagt acgcgtggat ccgcctgctg
    15061 gcgggcgaca ccggcaaagt gatgatcgtc ggtgatgacg accagtcaat ctacggctgg
    15121 cgcggggcgc aggtggagaa tattcagcgt ttccttaatg atttccccgg tgccgaaatt
    15181 attcgtctgg agcaaaacta ccgctctacc agcaatattc tgagcgccgc taacgccctg
    15241 attgaaaaca ataacgggcg tctgggtaaa aaactgtgga ccgatggcgc ggacggtgag
    15301 cctatttccc tctattgcgc ttttaacgaa ctcgatgaag cgcgttttgt ggttaaccgc
    15361 atcaaaacct ggcaggacaa cggcggggcg cttgccgagt gcgccattct ctaccgcagt
    15421 aacgcccagt cgcgtgtgct ggaagaagca ctgttgcagg cgagtatgcc gtaccgtatt
    15481 tacggcggga tgcgcttctt cgaacgccag gaaatcaaag atgcgctctc gtatctgcgc
    15541 ctgattgcca accgtaacga cgacgcggcc tttgagcgtg tggtgaatac gccaacgcgg
    15601 ggtattggtg accggacgct ggacgtggta cgtcagacat cgcgcgatcg ccagttaaca
    15661 ctctggcagg catgtagtga actgttgcag gaaaaagccc tcgccggacg tgctgccagc
    15721 gccttacagc ggtttatgga actgatcgac gccttagcgc aggaaactgc cgatatgccg
    15781 ctgcatgtac agactgaccg ggtaattaaa gactccggcc tgcgtaccat gtacgagcag
    15841 gagaagggcg aaaaaggtca gacgcgtatc gaaaacttag aggaactggt gacggcaacg
    15901 cggcagttca gctacaacga agaagacgaa gatttaatgc cgctgcaggc gttcctatcc
    15961 catgcggcgc tggaagcagg cgaagggcag gcggatacct ggcaggatgc ggtacaactg
    16021 atgaccatgc actcggcgaa aggtctggag ttcccgcagg tgtttatcgt cggtatggaa
    16081 gagggcatgt tcccaagcca gatgtcgctg gatgaaggcg ggcgtctgga agaagaacgc
    16141 cgtctggcct acgttggcgt aacccgcgcg atgcagaaac tgacgctgac ctacgcggaa
    16201 actcgccgtc tgtatggcaa agaggtttac catcgcccgt cgcgctttat tggcgagttg
    16261 ccggaagagt gtgtggaaga ggtgcgcctg cgcgccacgg taagccgccc ggtcagccat
    16321 cagcggatgg gtacgccgat ggtcgagaac gacagcggct acaagctcgg ccagcgtgta
    16381 cgccacgcta agtttggtga aggcaccatc gtcaatatgg aaggcagcgg tgaacatagc
    16441 cgtttgcagg tggcattcca gggccaggga atcaaatggc tggtggcagc ttacgcccgg
    16501 ctggagtcgg tgtaacgttg ccggatgcgg tgctgcgcac cttatttggc ctaaaaaatc
    16561 attcagattc aataaattgc aacgtcatgt aggcaggata aggcgtttac gccgcatccg
    16621 gcatcagcag catatttagt atctgacaca aagctatcat tctaaccttt ccgctcgacg
    16681 gaaatcatgg tgacaaaagg atagcgttgc cacgggattg ccccgccttt catatacata
    16741 tgtgaaatcg tgccaccaag ataaagcagc tgctcaacgt tcagtttcgc tttggcataa
    16801 caggcaaaat cataaaaatt tgtcgcctgc tggctcaaca aaaacacggc attcccatgt
    16861 ttattaatcc caacaccgtt acgaatttta cgtgaggcga cgttgggatg aatacgcgga
    16921 ttaattacac cgttttccat caacattggt cctgactgca ccgcaaactg aatatcttta
    16981 ctggctttaa aagcatccag acgaacgatg ccgactttat ctcccgcgac ataaaacacg
    17041 ccgccaggac ggataaagaa attcccttca cctgaagcga gatttaacgc taccttctg
//
LOCUS       DADMEQ010000082        16751 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.083, whole genome shotgun sequence.
ACCESSION   DADMEQ010000082 DADMEQ010000000
VERSION     DADMEQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16751
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.083"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="JNZ05_002723"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="JNZ05_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY8565900.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTMSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVHNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="JNZ05_002724"
     CDS             2219..2767
                     /locus_tag="JNZ05_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HAY8565901.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="JNZ05_002725"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="JNZ05_002725"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HAY8565902.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QSLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /locus_tag="JNZ05_002726"
     CDS             complement(3664..4854)
                     /locus_tag="JNZ05_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HAY8565903.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFSAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JNZ05_002727"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JNZ05_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HAY8565904.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="JNZ05_002728"
     CDS             complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="JNZ05_002728"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAY8565905.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8298..9500)
                     /gene="pncB"
                     /locus_tag="JNZ05_002729"
     CDS             complement(8298..9500)
                     /gene="pncB"
                     /locus_tag="JNZ05_002729"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAY8565906.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9766..12378
                     /gene="pepN"
                     /locus_tag="JNZ05_002730"
     CDS             9766..12378
                     /gene="pepN"
                     /locus_tag="JNZ05_002730"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAY8565907.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12585..13352)
                     /gene="ssuB"
                     /locus_tag="JNZ05_002731"
     CDS             complement(12585..13352)
                     /gene="ssuB"
                     /locus_tag="JNZ05_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY8565908.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLFDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13349..13996)
                     /locus_tag="JNZ05_002732"
     CDS             complement(13349..13996)
                     /locus_tag="JNZ05_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY8565909.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNI
                     DRGLVEMARSYGLSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGI
                     GYLAINAREFLQTDVVVVAIILYALLGKLADVSAQLLERLWLSWNPAYHLKEATI"
     gene            complement(14008..15153)
                     /gene="ssuD"
                     /locus_tag="JNZ05_002733"
     CDS             complement(14008..15153)
                     /gene="ssuD"
                     /locus_tag="JNZ05_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HAY8565910.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSEHYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(15150..16109)
                     /locus_tag="JNZ05_002734"
     CDS             complement(15150..16109)
                     /locus_tag="JNZ05_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8565911.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPAGARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16102..16677)
                     /gene="ssuE"
                     /locus_tag="JNZ05_002735"
     CDS             complement(16102..16677)
                     /gene="ssuE"
                     /locus_tag="JNZ05_002735"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HAY8565912.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQPH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
BASE COUNT         4182 a         4320 c         4287 g         3962 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga tgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cacaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgctctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agtcgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgcctctgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg agaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcag ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaattatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt tttatgacac ctgccactgc cgtcaataag
     6181 ttctgtcaat aaaaatttac ggaactattg atgagagttt ggtgtcttta tgtgtctgca
     6241 ggcatctttc cattcaaact aacgtttcgc taccgtgaaa gtgctacaaa gataaagatt
     6301 tagtttcaaa aagaaaaaat atgtaaccaa aagtaaaaat taaggagctt tgtgaacacc
     6361 gtcatatttc catagaaacg tgatgatatt tacagcaatt tttatctatt tatatgattt
     6421 acttatattt aaattaacta aacggaaatt ttgtttctga tggaaacttt atcgacctgg
     6481 cacaaaatcc tctttcagac atccagaatg ccaaaaaata gtatgaaaat tgtgctatca
     6541 gctaagaaaa aacgaatccg caataagaat gcgggttatt ttttgtgcgt aaatgttgca
     6601 atgtaatatt ccgtaacagg atgatcgtta tggcgggggt gcaaatagat tgcttgactt
     6661 aacaaacaga taacttgaca gaaaggataa aaaagagcca gcaggcgctg gctctttgag
     6721 gaagaaaatc agaagctggc gttgcgcgga gtacgtggga acggaatcac atcacgcacg
     6781 ttttgcacgc cagttacgta agcaatcagg cgttcaaaac caagaccgaa acctgaatgc
     6841 ggaacagtac cgtagcgacg cagatcgcga taccaccagt aatcttcttt attcaggccc
     6901 atttccagca tacgctcgtc cagaacgtcc agacgttctt cacgctggga gccaccaatg
     6961 atctcaccga tgcccggagc cagaacgtcc atagccgcaa cggttttacc gtcttcgttg
     7021 aggcgcatat agaacgcttt aatatctttc ggatagtttt taaccactac cggtgcttta
     7081 aagtgttctt ccgccagata acgctcatgc tcagaagaga gatcgacgcc ccagtaaacc
     7141 gggttttcaa acttcctgcc gcagttttcg agaatggtca ctgcgtcggt gtaatccacc
     7201 tgtgcgaaat cggcttcaat gaagcgttcc agacgtgaaa cggcatcttt atctacgcgt
     7261 tcagcgaaga atttcatgtc gtctgcgcgt tcttcgagaa ccgctttgaa gacatatttc
     7321 agcatggctt cagccagacc cgcgatatcg ttcaggttag caaacgccac ttccggctcc
     7381 agcatccaga attccgccag gtgacggctg gtgttggagt tttcagcacg gaaagtcggg
     7441 ccgaaggtat aaattttgga taatgcgcaa gcgtaggttt cgccgttcaa ctggccagat
     7501 acggtcagga aagactcttt accaaagaag tctttgtcga aatccacttt gccctgatcg
     7561 ttacgcggca ggttttccag atccagcgta gaaacgcgga acatttcgcc tgcaccttcg
     7621 gtatcagatg cggtaatcag tggcgttgaa acccagaaga atccctgctc gttaaagaag
     7681 cgatgcagcg cctgcgccag cgtatggcga acgcgcgcga cggcaccaat caggtttgtg
     7741 cgcggacgca ggtgagcgac ttcacgcaga tactcaatgc tgtggcgttt tgccgccatc
     7801 gggtaagtgt ctggatcttc aacccaacca gcaacttcaa ccttgctggc ctgaatttca
     7861 aattgttgcc cctggcccgg cgatgccacg actttacccg tcacaatgac cgagcagccg
     7921 gtggtcagac gcaggacgtc ttcattgtaa ttgggcagag aattattgat gacagcctgt
     7981 acaggatcaa agcaggaacc gtcataaacg gcgaggaagg agatgccagc ttttgaatct
     8041 cggcgggtac gtacccatcc gcgtacggtg acttcgctgt caacggctac gcggccctgg
     8101 agtacgtcgg ctacaggcac aacgctcata atattctctc tgttaatagt cggaaaaaat
     8161 aaacacttgt ccacccgtaa atggggggga tacctatgtt acctggcatc tgcaatcaga
     8221 caagcagaat tcgcaaatgc agcgaaagat ttcggaatta aaggtaaaaa agggagccga
     8281 ttagctccct gatgatatta actggctttt ttaatatgcg gaaggtcgaa cgctttgcgc
     8341 agcgcccgaa caaacgcttt atcatggcag atagttttgc cagggctgtc agaaagtttc
     8401 gccaccggtt taccgttaca ctctaccaac ttaatcacaa tattcagggg ttttacctgg
     8461 gggatatcgc aggtcaggcg agtcccaata ccaaaactta attgcacgcg ggaagagaag
     8521 tggcggtata gctcaaccgc tttgcgtaaa tccagattgt cagagaaaac cagcgtttta
     8581 ctctgtggat caattcccag cttttcataa tgtgcaatgg ctttttcacc ccattcaacc
     8641 gggtcgccag agtcatgacg caggccctga taccgactag cgaactcgac accgaaatca
     8701 cgcaggaaag catccatagt gatgcagtcg gttaatgcaa tgccaagttg gtcgggatac
     8761 tcttccagcc aggcagcaag tgcagctcgc tggctgttgg ctagatccgg gctgatttgc
     8821 tgatgtgcct ggaaccattc gtgtgcctgt gttcccatcg gcgtgaggga aagccgacgc
     8881 gccagatcgt agttgctggt gcccacgaac caggattcct gttgcagacg cttaacgatg
     8941 gtttcttgta cttcgcgaga aaaacggcga cgggtgccaa aatccatcag atggaagcgc
     9001 gacatatcaa gaccggcggt taacgccgag aagtcgacta atttgctttc cagcgtgtcg
     9061 agggcttgtg caacgtcggc ctgcggtgag cgatagcgat gtaccatttc actgataacc
     9121 gccagcaaag gaacttccca gaggatgact tcacgccacg gaccgcttaa gcgaatatcc
     9181 agcttgccat tatcgttgga cacggtgact tgttccgggt taaagcggaa ctcgcgtaac
     9241 cagttaagat agtcggcctt aaagaaaggc agggcagaga gccactgata ttcatcatcc
     9301 tgcaggcgca ggtgctgcat cgcctgaatc tgttcacgaa tagcatcggc ataaataccc
     9361 agcagatcgt cacctcggca acgaaactcc gccgcgacat gcacatcgta atagtgatga
     9421 aacacggctt gctgcatatg caacttataa gcatctgtat ccagcaacga gtgcagaaca
     9481 ggagaagcga attgtgtcat aggtgcgcag tagcgtcctc gtacaggagc gtttagtaca
     9541 ataaacatct tcaggaactg ctggagtata ccttgtttcg caatttattg aaccccgatc
     9601 acaccatatg ccacctttct ggtcgatggc attacgcggt gtatgttata aaaatgtagc
     9661 aataaaggcg tttgtacctg aaaagatgaa gattctgcat agcgcgattt acgcaacagg
     9721 aatagactga acaccagact ctataaaaga tgctaaaggt tatttatgac tcaacagcca
     9781 caagccaaat accgtcacga ttatcgtgcg ccggattacc agattactga tattgacttg
     9841 acctttgacc tcgacgcgca aaagacggtc gttaccgcgg tcagccaggc tgtccgtcat
     9901 ggtgcatcag atgctccgct tcgtctcaac ggcgaagacc tcaaactggt ttctgttcat
     9961 attaatgatg agccgtggac cgcctggaaa gaagaagagg gcgcactggt catcagtaat
    10021 ttgccggagc gttttacgct taagatcatt aatgaaataa gcccggcggc gaatacggcg
    10081 ctggaagggc tttatcagtc aggcgatgcg ctttgcaccc agtgtgaagc cgaaggtttc
    10141 cgccatatta cgtattatct cgaccgcccg gacgtgctgg cgcgttttac caccaaaatt
    10201 attgccgata aaatcaaata tcccttcctg ctttccaatg gtaaccgcgt tgcgcaaggc
    10261 gaactcgaaa acggacgcca ttgggtacag tggcaggacc cgttcccgaa accgtgctac
    10321 ctgtttgcgc tggtggcagg cgactttgat gtactgcgcg atacctttac cacgcgttct
    10381 ggtcgcgaag tagcactgga gctgtacgtc gatcgcggca accttgatcg cgcgccgtgg
    10441 gcgatgacct cgctgaaaaa ctccatgaaa tgggatgaag aacgctttgg cctggagtat
    10501 gacctcgaca tctatatgat cgtcgcggtg gatttcttca atatgggcgc aatggagaat
    10561 aagggtctga atatctttaa ctccaaatat gtgctggccc gcaccgacac cgccaccgac
    10621 aaagattacc tcgatattga acgcgttatc ggccatgaat atttccataa ctggaccggt
    10681 aaccgagtga cctgccgcga ctggttccag ctcagcctga aagaaggttt aaccgtcttc
    10741 cgcgatcagg aattcagctc tgaccttggt tcccgcgcag ttaaccgcat caataatgtg
    10801 cgcaccatgc gcggattgca gtttgcggaa gacgccagcc cgatggcgca cccaatccgc
    10861 ccggacatgg tcattgagat gaacaacttc tacaccctga ccgtttacga gaagggcgcg
    10921 gaagtgatcc gtatgatcca caccctgctg ggcgaagaaa acttccagaa agggatgcaa
    10981 ctctatttcg agcgtcatga tggtagcgca gcgacctgtg acgactttgt gcaggcgatg
    11041 gaagatgcgt cgaatgttga tctctcccat ttccgccgtt ggtacagcca gtccggtacg
    11101 ccggttgtga ccgtcaaaga cgactacaat ccggaaactg agcagtacac cctgaccatc
    11161 agccagcgca cgccagccac gccggatcag gcagaaaaac agccgctgca tattccgttt
    11221 gccatcgaat tgtatgacaa cgaaggcaaa gtgatcccgt tgcagaaagg cggtcatccg
    11281 gtgaattccg tgctgaacgt cacccaggcg gaacagacct ttgtttttga taatgtctac
    11341 ttccagccgg tgcctgcgct gctgtgcgaa ttctctgcgc cagtgaaact ggaatataag
    11401 tggagcgatc agcaactgac cttcctgatg cgtcatgcgc gtaatgattt ctcccgttgg
    11461 gatgcggcgc aaagtttgct ggcaacctac atcaagctga acgtcgcccg tcatcagcaa
    11521 gggcagccgc tgtctctgcc ggtacatgtg gctgacgctt tccgcgcggt gctgctcgat
    11581 gagaagattg atccggcgct ggcggcagaa atcctgacgc tgccttctgt caatgaaatg
    11641 gctgaactgt tcgatatcat agacccgatt gctattgccg aagtacgcga agcactcact
    11701 cgtactctgg cgactgaact ggcggatgag ctgctggcta tttacaacgc gaattaccag
    11761 agcgagtacc gtgttgagca tgaagatatt gcaaaacgca ctctgcgtaa tgcctgcctg
    11821 cgcttccttg cttttggtga aacgcatctg gctgacgtgc tggtgagcaa gcaataccat
    11881 gaagcaaaca atatgaccga tgcgctggca gcgctttctg cggcggttgc tgcacagctg
    11941 ccttgccgtg acgcgctgat gcaggagtac gacgataagt ggcatcagga cggtctggtg
    12001 atggataaat ggtttatcct gcaagccacc agcccggcgg cgaatgtgct ggagacggtg
    12061 cgtggtctgt tgcagcatcg ctcatttacc atgagcaacc cgaaccgtat tcgttcgttg
    12121 attggtgcgt ttgcgggcag caacccggca gcgttccatg ccgaagatgg cagcggttac
    12181 cagttcctgg tggaaatgct taccgacctc aacagccgta acccgcaggt agcttcacgt
    12241 ctgattgaac cgctgattcg cctgaaacgt tatgatgcca aacgtcagga gaaaatgcgc
    12301 gcggcgctgg aacagttgaa agggctggaa aatctctctg gcgatcttta cgagaagata
    12361 actaaagcac tggcttgata cttttgagcc aaaatgcctg atgcgacgct tgacgcgtct
    12421 tatcaggcct acaactccgt tgaaattgct ggttttgtag gctggataag gcgttcacgc
    12481 cgcatccggc agccgtgccc aactgcctga tgcgatgctt gacgtgtctt atcaggccta
    12541 caagtccgtt gaaaatgctg gttttgtgcg ccacatccgg catattaacc atgacgccga
    12601 atcagttgtt cagaatgccc acgccgcatt acccgttgca atacttccgc ttccagctcc
    12661 gctaatcgca ccgaccctaa tcgacgcgga cgggggatat ccactgtcaa atccagacta
    12721 atttttccct cttcaattaa caacacccgg tcagccattg ctaccgcttc gctcacatca
    12781 tgcgtcacca acagtacggt aaagccgtgc tgctgccaaa gtgacacaat caaatcctgc
    12841 atctcgagtc gcgttaaggc gtccagtgcc ccgagcggtt catcaaacaa caataaaccc
    12901 ggtcgatgaa tcaacgctct tgccagcgcc acacgctgtt tctgcccgcc agaaagtgcg
    12961 gcaggccatt ccccggcgcg attctccagt cctaccgcag ccaacgcccg acgcgcggca
    13021 tcgcgccact gacctttaag gcctaaccca acgttatcaa tcaccgattt ccacggcagc
    13081 agacgcgcat cctgaaacat cattcgcgta tcatcctgaa tttcagccag cggtgtggtg
    13141 cccgctaaca catcgcctgc ggttggcgtt tccagaccgg ccagcaggcg cagcagggta
    13201 cttttaccac caccgctgcg gcccaccacc gccacaaact gacctgccgg aatatgtaaa
    13261 tccagttggt tcaggacgat attttccgcg taatgtttgc ttactgcatt gagcaacaat
    13321 ggtgtgccct ggttcagacg agcagtattc atatcgtggc ctccttcaaa tgataagccg
    13381 ggttccagct caaccagagg cgctctaaca gctgcgcgct gacgtcagca agtttgccga
    13441 gcagggcgta aagaataata gcgactacca ccacgtctgt ttgcaaaaac tctcgcgcat
    13501 ttatcgccag ataaccaatg ccggaattgg cagaaatggt ttcggcaaca atcagcgtca
    13561 gccacatcag gcccaacgca aaacgcacgc cgaccataat tgagggcagg gcatcgggca
    13621 ggatcacatg gataaacagc ggtatgccgg ataatccata gctacgcgcc atctccacca
    13681 gcccgcgatc gatattacgg atgccatgcc aggtgttgat ataaatgggg aacagcgtac
    13741 cgagcgccac cagaaagatt ttcgcggatt catcaatgcc aaaccacaaa atcaccagcg
    13801 ggatcagcgc cagatgctgc cacagttcgc cgctggcgga gagcgtccag aacgccgtca
    13861 ccactccttc cggtgaaggc aaaatacgcg tcgacagcca gccaaccgag gaggccagtt
    13921 gccacaccgc cacgatgccc accggtaaaa accagggggc aacgcgcaat aaccacttct
    13981 tcactggcgt tgccatcatc gtgttcctta gctttgcgcg actttacggg ggataaaatc
    14041 attcgccacc gcttcgcctt gcggattcag cggctgcggc tggggaattt ccggaatggc
    14101 gacatccaga tgtgggaaca gcaactcgcc aacccgatac gcttcttcca gatgcgggta
    14161 gcccgaaagc acaaagctgt cgatgccaag cgcggcgtat tcgttgattc gcgcggcgac
    14221 cgtaggacca tcgcccacca gcgccgttcc ggcaccgccg cgcactaagc caacgcccgc
    14281 ccataaattg gggctgatct ccagattgtc gcgcttgccg ttatgtagcg ccgccattcg
    14341 ctgttgtcct acggaatccg tccgggcgaa tgcggcttgt gctttggcga tagtctcatc
    14401 atcaagatgc gagattaacc gctcggcggc ctgccacgct tcgtcgttag tttcacgaac
    14461 aatcacatgc agacgaatac cgaaacgaat tttgcgccca tacgcggcag ctttcgcccg
    14521 cacttgttcg attttctctt taacaagttc cggcggttcg ccccaggtga ggtagagatc
    14581 aacttgttct gccgccagct cctgggcgac atctgacgat ccgccaaagt aaagcggagg
    14641 ataaggctgt tgaatcggcg ggaagagcag ttttgctccg cgcacatgaa tatgtttacc
    14701 gttgaaattg acggtttccc ccagcaataa gcgccgccag acctgggtaa attccgccga
    14761 ggcttcgtag tgctcgctat gatcaaggaa cacgccgtcg cctgccagct cttgtggatc
    14821 gctgcctgtg accagattaa acaacgcacg accatttgag agacggtcaa gcgtggcggc
    14881 ctggcgggcg gcaacagtag gtgaggttac gctgggacgc agggcgacaa gaaacttcag
    14941 ccgctgcgtc accgggatca tcgacgctgc aaccagccac gcatcttcac acgatcgccc
    15001 cgttgggatc agcacaccgg tatagcccag acgatccgcc gcttgcgcaa tttgttgcag
    15061 ataaccgtga tcaaccgggc gtgaaccttc ttccgttccc agatagtgcc cgtcaccgtg
    15121 ggtcggtaaa aaccagaaca tattcagact cataattgtt ttccttccag ttgagtgggc
    15181 tgccagatgc gctggcgaat atcgactttt ttcggcacca gacggttttc ataaaacaga
    15241 tctgccgttt gctgctgtaa ggcggcaacc tcggcgttaa ccggtttgat ggtggtagga
    15301 gggcgatgat ctaagtaaga ggcaatcacc ggtgccggta agcccatcgt ttttgccagt
    15361 aaagcgatgc tttgttcgcg ctggctgcgg gttaacgcat cggcctcact aaaggttgcc
    15421 agtacgccct gaataaaagc gccgtttttt tctgcatagg gacgagctgc cagataaaac
    15481 gatccagttt gattgagatc ggtgccgtct ttcagcaccc gcacgccgcc ctgtaataac
    15541 gcagcggagt agtagggatc ccagatagcc caggcgtcaa cgttaccttg ctggaacgcg
    15601 gcgcgggcac cggcaggcgt caggtaagtg ggctggatat cggtgaattt aagtccggcc
    15661 tgacgcagtg cacgcagtaa aaggttgtgt gaactggaac ctttctgaaa ggcaactttg
    15721 tgacctttaa gatcggctac ggttttgatc gggctgtttt ctgccaccag aatcacttcg
    15781 gctttgggct tcggtggctc gacgcccaca tataccaaat cagccccggc agcctgggca
    15841 aagattggcg gaatatcccc ggtactgccg agatcaatac tgccaacgtt taacgcttcc
    15901 aacatttgcg gacccgcggg gaactccacc caggagattt ttgattgcgg atagcgtttt
    15961 tccagtaact ggtggctttt tgccagcacc ataccaatac tgcctttctg atagcctata
    16021 cgtaacgctt caggcgagga ttctgcagca accgcaaaag tagagacgct aagcaatccc
    16081 gccagcgcca gtttaatgat gttacgcatg ggcattacct cgcagagaca gaaggtcagg
    16141 aacctgaaca tcgcggcggt gcaatgcctg ccagaaagtt tctagcgcgg tatcaagatg
    16201 gggttgcaga tttggcgtga actggggtct gtgatggtaa tcaattaatt gtgagtcatc
    16261 ggcaaacacg ccgtgcagga tctcctgagc tttcagtgcg cttaaaactg gtttaagggc
    16321 ataatcgacc gccagcagat gggccacggt accgcccgtc gccagcggta gcaccacttt
    16381 gccttgcaaa gcgcgctctg gcagcaggtc gagcagggtt ttcagcgcgc cggaataggc
    16441 ggctttatac acaggcgtgg cgacaatcag cccatcagcc tgttgcagct gttcggtgaa
    16501 ggtcttgagt gccggactat cgaaacgagc ataaagcaga tcttccgggg cgaagttttg
    16561 cagattccag tgataaacct ctacatctag gccatttagt ttttcccgcg catattccag
    16621 caaggagctg gagcgagaag gaaagcgagg actacccgcc agggtgatga cacgcatact
    16681 ctctccttat aaccaattgt tctttttttt gttaacattg ataacaattc ggtcagtctg
    16741 tcggagagac a
//
LOCUS       DADMEQ010000083        16429 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.084, whole genome shotgun sequence.
ACCESSION   DADMEQ010000083 DADMEQ010000000
VERSION     DADMEQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.084"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..83
                     /locus_tag="JNZ05_002736"
     CDS             <1..83
                     /locus_tag="JNZ05_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAY8565913.1"
                     /translation="TPGQVENRALYPRPEGWGFTAHRIKD"
     gene            complement(122..469)
                     /gene="grxD"
                     /locus_tag="JNZ05_002737"
     CDS             complement(122..469)
                     /gene="grxD"
                     /locus_tag="JNZ05_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HAY8565914.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            804..1631
                     /locus_tag="JNZ05_002738"
     CDS             804..1631
                     /locus_tag="JNZ05_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HAY8565915.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATAHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1759..2340
                     /gene="sodB"
                     /locus_tag="JNZ05_002739"
     CDS             1759..2340
                     /gene="sodB"
                     /locus_tag="JNZ05_002739"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HAY8565916.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(2486..3637)
                     /locus_tag="JNZ05_002740"
                     /pseudo
     CDS             complement(2486..3637)
                     /locus_tag="JNZ05_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3803..3892)
                     /gene="ynhF"
                     /locus_tag="JNZ05_002741"
     CDS             complement(3803..3892)
                     /gene="ynhF"
                     /locus_tag="JNZ05_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HAY8565917.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            4191..5216
                     /gene="purR"
                     /locus_tag="JNZ05_002742"
     CDS             4191..5216
                     /gene="purR"
                     /locus_tag="JNZ05_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HAY8565918.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(5213..6145)
                     /locus_tag="JNZ05_002743"
     CDS             complement(5213..6145)
                     /locus_tag="JNZ05_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8565919.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            6258..7469
                     /locus_tag="JNZ05_002744"
     CDS             6258..7469
                     /locus_tag="JNZ05_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HAY8565920.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRTALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            7760..8908
                     /gene="cfa"
                     /locus_tag="JNZ05_002745"
     CDS             7760..8908
                     /gene="cfa"
                     /locus_tag="JNZ05_002745"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HAY8565921.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(8948..9589)
                     /gene="ribE"
                     /locus_tag="JNZ05_002746"
     CDS             complement(8948..9589)
                     /gene="ribE"
                     /locus_tag="JNZ05_002746"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAY8565922.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            9804..11177
                     /gene="mdtK"
                     /locus_tag="JNZ05_002747"
     CDS             9804..11177
                     /gene="mdtK"
                     /locus_tag="JNZ05_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HAY8565923.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFISLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(11218..12474)
                     /locus_tag="JNZ05_002748"
     CDS             complement(11218..12474)
                     /locus_tag="JNZ05_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565924.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYAYGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYCLGT
                     DGLQLYSSGKTQNLSVNVGDRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            12782..12858
                     /locus_tag="JNZ05_002749"
     tRNA            12782..12858
                     /locus_tag="JNZ05_002749"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12816..12818,aa:Val,seq:gac)
     gene            12863..12939
                     /locus_tag="JNZ05_002750"
     tRNA            12863..12939
                     /locus_tag="JNZ05_002750"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12897..12899,aa:Val,seq:gac)
     gene            13047..13352
                     /locus_tag="JNZ05_002751"
     CDS             13047..13352
                     /locus_tag="JNZ05_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HAY8565925.1"
                     /translation="MATLLQFHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            13478..15082
                     /locus_tag="JNZ05_002752"
     CDS             13478..15082
                     /locus_tag="JNZ05_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HAY8565926.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPCEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(15094..15906)
                     /locus_tag="JNZ05_002753"
     CDS             complement(15094..15906)
                     /locus_tag="JNZ05_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565927.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(15910..16429)
                     /locus_tag="JNZ05_002754"
                     /pseudo
     CDS             complement(15910..16429)
                     /locus_tag="JNZ05_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
BASE COUNT         4062 a         4251 c         3906 g         4210 t
ORIGIN      
        1 aaacacctgg tcaggtggaa aaccgcgcct tatatccccg ccctgaagga tggggtttta
       61 cggcgcaccg gataaaggac tgacggatgc ccgttcgcat ccgtcagtat tgcaggacgg
      121 attattccgc gtccggctct tcagacttgt atttagcggc agtttctttg atcagctgct
      181 gcagttcgcc acgctgatac atttcgatca cgatatcaca accgccgacc agctcgccgt
      241 caacccacag ttgcgggaag gtcggccagt tagcatattt cggcagttcc gcacgaatgt
      301 ccggattctg cagaatatca acataggcaa aacgttcgcc acatgcggca agcgcctgga
      361 ctgcctgggc agagaaaccg cagctcggca gtttcggtga acctttcatg tacagcagga
      421 tcgggttttc agcaatctgg cgttggattt tttcgatagt ggtgctcatt gtcttgcttc
      481 ctcaaacttc tttacggcag taatctgaca ttgtagcggg tcagtgcggc atcggaaaat
      541 aacattttca tcacgctttt gctattttat ccctttgctc tatttgttgc atttcaaata
      601 ttcgtttctt ctctgatgcg cggcaaactg gcgtatttat aaccattttt agctttcact
      661 gctgcatttt tttgcgctcg ccaacgaaac gtatttttta acaataaaag ctattaactt
      721 tctcttcttc tatgcattag aatcatcaag ttttgtaaat cagacgcagg catgatagac
      781 ctgccttaac agagggacgc tcagtggcgc ggataaaccg tatttcgatc acgctctgtg
      841 ctttactttt taccaccctg cctttaacgc ctatggccca tgcttcaaag caagccaggg
      901 agagttctgc taccgctcat atcaccaaaa aagcagataa aaagaaaagc acggcaacca
      961 ccaaaaagac ccagaaagcc agccagaaaa cagcgaaaaa agccgccagt aaaagtacga
     1021 ccaaaagcaa aaccgcttct tccgttaaaa aatcttccat taccgcttct aaaaacgcca
     1081 aaactcgcag caaacacgcc gtcaataaaa cggcctcagc cagctttacc gaaaagtgta
     1141 ccaaacgtaa gggctataaa tcgcattgtg tgaaagtcaa aaatgccgcg tcaggaaccc
     1201 ttgccgacgc gcacaaagcg aaggtgcaaa aagccacgaa agtggcaatg aataaactga
     1261 tgcagcaaat tggtaagcca tatcgttggg gcggcagctc accgcgtacc ggttttgatt
     1321 gcagcggcct ggtttattac gcttataaag atttggtgaa aattcgtatt ccgcgtacgg
     1381 cgaatgaaat gtatcacctg cgtgatgcag gacctatcga acgtagtgag ctgaaaaacg
     1441 gcgacctggt ctttttccgt actcagggac gcggtacagc cgatcatgtc ggcgtgtatg
     1501 tcggcaacgg caaatttatt cagtcaccgc gcacaggtca ggaaattcaa atcacttctc
     1561 tcagtgaaga ctactggcag cgccactatg ttggcgctcg tcgggtaatg accccaaaaa
     1621 cacttcgcta aaactttacc ctgttgttac ggcaacaggg taagttcatc ttttgtctca
     1681 ccttttaatt tgctacccta tccatacgca caataaggct attgtacgta tgcaaattaa
     1741 taataaagga gagtagcaat gtcattcgaa ttacctgcac taccatatgc taaagatgct
     1801 ctggcaccgc acatttctgc ggaaactatc gagtatcact acggcaagca tcatcagact
     1861 tatgtcacta acctgaacaa cctgattaaa ggtaccgcgt ttgaaggtaa atcactggaa
     1921 gagattattc gcagctctga aggtggcgta ttcaacaacg cagctcaggt ctggaaccat
     1981 actttctact ggaactgcct ggcaccgaac gccggtggcg aaccgactgg aaaagtcgct
     2041 gaagctatcg ccgcatcttt tggcagcttt gccgatttca aagcgcagtt tactgatgca
     2101 gcgatcaaaa actttggttc tggctggacc tggctggtga aaaacagcga tggcaaactg
     2161 gctatcgttt caacctctaa cgcgggtact ccgctgacca ccgatgcgac tccgctgctg
     2221 accgttgatg tctgggaaca cgcttattac atcgactatc gcaatgcacg tcctggctat
     2281 ctggagcact tctgggcgct ggtgaactgg gaattcgtag cgaaaaatct cgctgcataa
     2341 taactgatgg caaatgcagc attgcctgat gcgcttcgct taccatgcca acgcggatga
     2401 ggcgttaaca gcgcattcgg caagataaat cgcacgttgt cagcaactgt aacgcagaag
     2461 gttatccttc tgcgtttttg tttaattagc tgttagcaac gcaaactgtt tcaggttgtt
     2521 ttctggctga cataaacacc agcaataatg ccagtcccgc gacaatcgcc cccgtcaccg
     2581 gcacaaagct gtatcccagc ccagcggaaa ttaccgcacc accagcagct gctcccagcg
     2641 catttccaag attaaaggca ccaatattga ctgatgaaga cagacctggc gcttcactgg
     2701 cgacatgcat cacgcgcatc tgtaacggag gtacaaccgc aaaggttgct gcgccccaca
     2761 ccaccatgct aatagctgcg ccgaactcat tgcgggccag gaacgggatt gccagcataa
     2821 tcaccatcag cagcaacaaa aagcctttca acgtgccgtt attgccgccg agatagttac
     2881 cgatagagaa tccgacacca atcagcacca gcattgccgt gacgaacacc ggtgttgcgt
     2941 gggtaatact ttgcagtacc ggagagatat aggtgtagag ggtaaacatt gcaccagctc
     3001 ccagtaccgt cgtcagcaat gcagacagca cctgcggacg cattaatacc gccagctctt
     3061 ttttcacttc aggtcgtgcc cctgtaccac ctttaggtaa tgagaagaac agacttacca
     3121 ttgaaatcac tcccagcccc gccgttgcca gaaatgacat ccgccagccg atggtttcac
     3181 ccaacctggt cgccgccggc acgccaccga tatttgccag ggttaacccc ataaacatag
     3241 tggcaactgc gctggcctgt ttatgttttg gcaccacgct tgcggccacg actgaaccca
     3301 aaccaaaaaa tgctcagtga ttcaggctgg tcaaaatacg tgaaagcatc agggtcatat
     3361 aatccggcgc gatggcggaa agaacgttgc ctagcgtgaa aattgccatc aggaaaatca
     3421 acgcactgcg gcgggcacga tgagaaagta gaagcgtcat cagcggcgcg cccaccatta
     3481 cgccaactgc ataggcactg attaacattc cggcagcggg aatcgagaca tccacaccgc
     3541 gcgcaatgac gggcaacaag cccattggcg agaactccgt tgtcccgata ccaaacgcgc
     3601 caatcgccag cgccagcaac ggatagttaa ttttcatgcc ttatctccac ctcttcgcgt
     3661 cattacgcga tattcattaa agtggcgaaa gcatgacagc aatcacaaaa aaatgaaaat
     3721 aacaaaaaga gaaaacactt ttgccatttt gctaacaaac aggaaggaga tgcgagggag
     3781 aacgcgctcc ctcgagagga aatcagtgca gcgcggcagt caaacccacg gctacgatca
     3841 aaccgaggac gataatcgtt gttaccagtg aaaatttaag gtcggtgctc atcaagtttt
     3901 ctcctttttt attaccacac aaaaaatgat attacgcatt tttacacact gtgatgaaaa
     3961 aatctcccgt catttataat gataagtgtt tttaccactt ccccttttcg tcaagatcgg
     4021 ccaaaattcc acgcttacac tatttgcgta ctggccattg accccttcct gacgctccgt
     4081 gtcgtttttc cggcgtaccg caacactttt gttgtgcgta aggtgtgtaa aggcaaacgt
     4141 ttaccttgcg attttgcagg agctgaagtt agggtctgga gtgaaatgga atggcaacaa
     4201 taaaagatgt agcgaaacga gcaaacgttt ccactacaac tgtgtcacac gtgatcaaca
     4261 aaacacgttt cgtcgctgaa gaaacgcgca acgccgtgtg ggcagcgatt aaagaattac
     4321 actactcccc tagcgcggtg gcgcgtagcc tgaaggttaa ccacaccaag tctatcggtt
     4381 tgctggcgac cagcagcgaa gcggcctatt ttgccgagat cattgaagca gttgaaaaaa
     4441 attgcttcca gaaaggttac accctgattc tgggtaatgc gtggaacaat cttgagaaac
     4501 agcgggctta tctgtcgatg atggcgcaaa aacgcgtcga tggtctgctg gtgatgtgtt
     4561 ctgagtaccc agagccgttg ctggcgatgc tggaagagta tcgccatatc ccaatggtgg
     4621 tgatggactg gggtgaagca aaagctgact tcaccgatgc ggtcattgat aacgcgttcg
     4681 aaggcggcta catggccggg cgttatctga ttgaacgcgg tcaccgcgaa atcggcgtca
     4741 tccccggccc gctggaacgt aacaccggcg caggccgcct tgccggtttt atgaaggcga
     4801 tggaagaagc gatgatcaag gtgccggaaa gctggattgt gcagggtgac tttgaacctg
     4861 aatccggtta tcgcgccatg cagcaaatcc tgtcgcagcc gcatcgccct actgccgtct
     4921 tctgtggtgg cgatatcatg gcaatgggcg cactttgtgc tgctgatgaa atgggcctgc
     4981 gcgtcccgca ggatgtttcg ctgatcggtt atgataacgt gcgcaacgcg cgctatttta
     5041 cgccggcgct gaccacgatc catcagccaa aagattcgct gggtgaaaca gcgttcaaca
     5101 tgctgttgga tcgtatcgtc aacaaacgtg aagaaccgca gtccattgaa gtgcatccgc
     5161 gtttgattga acgccgctcc gtggctgacg gcccgttccg cgactatcgt cgttaatcac
     5221 ccgttgcggg agtctcttcc ggctcccgca gccactcctt attcagcgtc tcactatcgc
     5281 cgagatactc aagcagccag gttaacgcag gcgacatatc attttgctgc catgtcagac
     5341 aacatgccga atccgggaag gggttttcca gttcaagtgc tacccacttc ccctcattaa
     5401 gccacggttt ggcgaaatgt gttggcacca tccctatgca taatcctgcc gagatacagg
     5461 ttgccgatga ttcccagtca ggtacgacaa gtcttttttg gttatccagc aaccaggtaa
     5521 tacgtttagg tagcgttcgc gaggtgtctt cgcgcaccaa cgacggccag ttgcgcaacg
     5581 tatcatcgct gaacgggcca tccatcaacg ccagcgggtg gtgactggca acaacgcaac
     5641 tccagcttag catccccata tcccggaagg cataacgacc gcctaccgga atcgcgcgtg
     5701 ttgcgccaat tgccagttcc acgcgcccgt cggaaagcgc atcccagaca ccgttgaaca
     5761 cttcctgaaa gacaagaagt tcaacatcat caaaatggcg ataaaaatca acgatcatct
     5821 gccgtgtacg ttctggcctg acaatattat ccactgcgat agctaactga ccgcgccagc
     5881 cgttcgctat ctgctgacat tgctggcggg tgatctgcat ttttttgaca acagagcgcc
     5941 cttctttgag aaaccacgct ccagcagcgg tcagctccac atcacggtgc cgtcgttcaa
     6001 agagcggtac cgccagccac tcttccagct gacgcacggt atagctgacc gcagaaggaa
     6061 cgcgatgcag ctcctgtgcc gcagcgctaa aactaccatt acgcgctacc gcatcaacaa
     6121 cttcgagtga atattctgac cacatagtca gcctgcaaaa tttttgaaac cagtcatcaa
     6181 atattaccgt ttcacaacac taatttcact ccctacactt tgcggcggtg tttaattgag
     6241 agatttagag aatatacatg caacctggga aaagattttt agtctggctg gcgggtttga
     6301 gcgtactcgg ttttctggca accgatatgt atctgcctgc tttcgccgcc atacaggccg
     6361 acctgcaaac gcctgcgtct gctgtcagtg ccagccttag tctgttcctt gccggttttg
     6421 ccgcagccca gcttctgtgg gggccactct ccgaccgtta tggtcgtaaa ccggtgttat
     6481 taatcggcct gacaattttt gcgttaggta gtctggggat gctgtgggta gaaaacgccg
     6541 ccacgctgct ggtattgcgt tttgtacagg ctgtgggggt ctgcgccgcg gcggttatct
     6601 ggcaagcatt agtgacagat tattatcctt cacagaaagt caaccgtatt tttgcgacca
     6661 tcatgccgct ggtgggtctg tctccggcac tggctcccct gttaggaagc tggctgctgg
     6721 tccatttttc ctggcaggcg attttcgcca ccctgtttgc cattaccgtg gtgctgattc
     6781 tgcctatttt ctggctcaaa cccacgacga aggcccgtaa caatagtcag gatggtctga
     6841 cctttaccga cctgctacgt tctaaaacct atcgcggcaa cgtgctgata tacgcagcct
     6901 gttcagccag tttttttgca tggctgaccg gttcaccgtt catccttagt gaaatgggct
     6961 acagcccggc agttattggt ttaagttatg tcccgcaaac tatcgcgttt ctgattggtg
     7021 gttatggctg tcgcaccgca ctgcagaaat ggcaaggcaa gcagttatta ccgtggttgc
     7081 tggtgctgtt tgctgtcagc gtcattgcga cctgggctgc gggcttcatt agccatgtgt
     7141 cgctggtcga aatcctgatc ccattctgtg tgatggcgat tgccaatggc gcgatctacc
     7201 ctattgttgt cgcccaggcg ctgcgtccct tcccacatgc aaccggtcgc gccgcagcgt
     7261 tgcagaacac tctgcaactg ggtctgtgct tcctcgcaag tctggtagtt tcctggctta
     7321 ttagtatcag cacgccattg ctcaccacca ccagcgtgat gttatcaaca gtagtgctgg
     7381 tcgcgctggg ttacatgatg caacgttgtg aagaagttga ctgcccgaat catggcaatg
     7441 ccgaagtcgc tcatagcgaa tcacactgat ctatatcgat atacttatac ttaggctgct
     7501 aacaaaattt tgttgtatga ttgaaattag cggcctatac taatttcgag ttgttaaagc
     7561 tacgataaat attatgtttt tacggggaca ggatcgttcc cgactcacta tggatggtca
     7621 tttcggcaag ggttcctcct ttccctctgt tctacgtcgg attatagact cgcggttttt
     7681 tctgcgagat ttctcacaaa gcccaaaaag cgtctacgct gttttaaggt tctgatcacc
     7741 gaccagtgat ggagaaacta tgagttcatc gtgtatagaa gaagtcagtg taccggatga
     7801 caactggtac cgtatcgcca acgaattact tagccgtgcc ggtatagcca ttaacggttc
     7861 tgccccggcg gatattcgtg tgaaaaaccc cgattttttt aaacgcgttc tgcaggaagg
     7921 ctctttgggg ttaggcgaaa gttatatgga tggctggtgg gaatgtgacc gactggatat
     7981 gttttttagc aaagtcttac gcgcaggtct cgagaaccaa ctcccccatc atttcaaaga
     8041 cacgctgcgt attgccggcg ctcgtctctt caatctgcag agtaaaaaac gtgcctggat
     8101 agtcggcaag gagcattacg atttgggtaa tgacttgttc agccgcatgc ttgatccctt
     8161 catgcaatat tcctgcgctt actggaaaga tgccgataat ctggaatctg cccagcaggc
     8221 gaagctcaaa atgatttgtg aaaaattgca gttaaaacca gggatgcgcg tactggatat
     8281 tggctgcggc tggggcggtc tggcgcacta catggcatct aactatgacg taagcgtggt
     8341 cggcgtcacc atttctgccg aacagcaaaa aatggctcag gaacgctgta aaggcctgga
     8401 tgtcaccatt ttgctgcaag attatcgtga cctgaacgac cagtttgatc gtattgtttc
     8461 tgtggggatg ttcgagcacg tcggaccgaa aaattacgat acctattttg cggtggtgga
     8521 tcgtaatttg aaaccggaag gtatattcct gctccatact atcggttcga aaaaaaccga
     8581 tctgaatgtt gatccctgga ttaataaata tatttttccg aacggttgcc tgccctctgt
     8641 acgccagatt gctcaatcca gcgaacccca ctttgtgatg gaagactggc ataacttcgg
     8701 tgctgattac gatactacgt tgatggcgtg gtatgaacga ttcctcgccg catggccaga
     8761 aattgcggat aactacagtg aacgctttaa acggatgttt acctattatc tgaatgcctg
     8821 cgcaggtgct ttccgcgccc gtgatattca gctctggcag gtggtgttct cacgcggtgt
     8881 tgaaaacggc cttcgagtcg ctcgctaaag gctattctat cgccccctct tcgggggcga
     8941 gttcagatca ggcttctgta cctggttgat tcatggcatt ttctcgtgcc gccagcacac
     9001 gttctaccgt atctaccact gcctgagttt gtggatcgat ttcaatgttg acgcgtgcgc
     9061 caagtttttt cttcccaaga gtcgtgcgtt ccagtgtttc cggaattaaa tggacgcaaa
     9121 aacgcgttgg cgtgacttcg ccgacggtca ggctaatacc gtcgatgcca ataaatcctt
     9181 tgtacaggat atatttcatc aactgactat cctggacttt aaaccagatc tggcgattat
     9241 tttccgaggt taatattttc gccacttcag cggtggtcat aatatgacct gacattaagt
     9301 gtccgccaat ttcatcactg aatttcgccg cacgctcaac gtttacccaa tcccccactt
     9361 ttaaatcgcc aagattggta atgcgtaacg tttctttcat caggtcaaaa ctgacatggt
     9421 tgccgttaat ttccgtcacg gtcaggcagc aaccgttatg cgccacggaa gcaccggttt
     9481 ccaggccgtc cagcatgtgg tcgggtaact ccaccacatg cgtacgaaaa tttggtttct
     9541 cgtcaatcga caccagtttt gcggtgccct gtacaatccc cgtaaacata cttacaactc
     9601 ctgaaatcag ttaagacatt ctgttcagca caatagcagg tggaaaacgc ccttaccagt
     9661 gaaggggtaa gaatggctat tttttcactg gagaattaat aaatcctcgc tacaatagac
     9721 tgaatttccc ctgcttcttc tttttgctgc ccattcaggc ggctttttag tctctcatat
     9781 aactacaaat aaaaggtgtt cacgtgcaga agtatatcag tgaagcgcgt ctgttattag
     9841 cattagcaat cccggtgatt ctcgcgcaaa tcgcccaaac tgcgatgggt tttgtcgata
     9901 ccgtgatggc gggcggctat agtgccaccg acatggcggc ggtcgctatc ggtacttcta
     9961 tctggcttcc ggcgatcctc tttggtcacg gactgctgct ggcattaacg ccggttatcg
    10021 cgcaattaaa tggttccggt cgacgtgagc gcattgcgca tcaggtgcga caaggtttct
    10081 ggctggcagg ttttgtttcc gttctcatta tgctggtgct gtggaatgca ggttacatta
    10141 tccgctccat ggaaaacatc gatccggctc tggcggacaa agccgtgggt tatctgcgtg
    10201 cgttgttgtg gggcgcgccg ggatatctgt tcttccaggt tgcccgtaac caatgtgaag
    10261 gtctggcaaa aaccaagccg ggtatggtaa tgggctttat cagcctgctg gtgaacatcc
    10321 cggtgaacta tatctttatt tatggtcatt tcggtatgcc tgagctcggt ggcgttggtt
    10381 gtggcgtggc tactgcggcg gtgtattggg tcatgttcct tgccatggtt tcttacatta
    10441 aacgcgcccg ctccatgcgc gatattcgta acgaaaaagg caccgctaag cccgatcctg
    10501 cggttatgaa acgactgatt caactcggtt tgccgattgc gctggcactg ttctttgaag
    10561 tgacactgtt tgccgtcgtg gctctgttag tgtctccgct cggtattgtt gatgtcgcag
    10621 gacaccagat tgccctgaac tttagttcac tgatgtttgt gcttccaatg tcgctggcgg
    10681 cagcggtaac tatccgcgta ggttatcgtc tgggtcaggg ctcaacgctg gatgcgcaaa
    10741 ccgctgcgcg gaccgggctt atggtgggtg tctgtatggc aaccctgacg gccattttca
    10801 cggtttcact gcgggagcaa atcgccctgt tgtacaacga caatcccgag gttgtaacgc
    10861 tggctgcgca tttgatgttg ctggcggcgg tatatcagat ttctgactca atccaggtga
    10921 ttggcagtgg gattttgcgt ggttataaag atacgcgttc cattttctat atcaccttta
    10981 cagcttactg ggtgctgggc ttgccaagcg gctatattct ggcactgacc gatctggtcg
    11041 ttgaacctat ggggccagca ggcttctgga taggctttat tatcggcctg acatcggcag
    11101 ccattatgat gatgctgcgt atgcggttcc tgcaacgtat gccgtcagcc atcattctgc
    11161 aacgagcgtc ccgctaataa agacaaggcg caaccttcac gggttgcgcc tgtgttttta
    11221 cgcaggctgg agcgttgcgc caatcccgtc ttcgtctggc tgtaatttca gagcgttaca
    11281 cagagtttgc cgactggcat ctgttatctc aacgcgtata tccccgttaa cttccccttt
    11341 cacattgatg aagtcaggtg aaatttcttt cgcactggtt tgcaggcaaa tcgctccctc
    11401 tccgcgcagg cgtttcactt tcagttgcac atgcgttgcc gcaccagtga tcagccacag
    11461 ttttccgcca ttcagattga tgttaccaac aatgaaagtg acgctgtcac cttttaccgt
    11521 actgccgtcg agaatcaaca caccgccctg ctgtacagta atcgttgatt gttgcagctc
    11581 tgcgccataa ccaataatca ttgaagcgcc gtccagtaat gcaacactcc cggtcagttc
    11641 aaccggtgcg cgatcttcct ctacgacaaa attttcgtcc gcgctggtgg gtgacaacag
    11701 gatcactgtt ccaccttgta ttaccgcatt ttccagcgta ccggcatgca cttcagcccg
    11761 atcccccaca ttcaccgaca ggttttgcgt cttaccggaa ctgtagagct gaaggccatc
    11821 cgtccccaga caataaatgg atccattctc aacaatagtg tcagagtctt caccaccgag
    11881 attattcatg gtgccaccag caagcaacgt atcactggct ttcccggcca gcataaaaac
    11941 gccgccctga ttaatctcac aattgctggc ttctccactt ggatacacca acaattcacc
    12001 accttcatct accacgaccg ctgaggttgt cccattaacc aacaggccgc cctcttgatc
    12061 gagaatgatt ttttccgcgc gatgtccttc cagtacacgc aggttaccgc cattttccag
    12121 caacaaaccg taggcataac cctgatcaac gctgaattca ccttcgggat ggcggccatt
    12181 aaccgtagcg agcgtagaga gactaattgc gcctccctcg gactgaacaa tatctgtggc
    12241 aatggcaccg tcgctgaccg atagcgtgcc attgttaccg tactgtgtgc atttggcgat
    12301 cccaccggtt gccactttca accagccagc ccccgtgacc acggtatttt cggctcttcc
    12361 gccagcttca acaatcaact cgccttcagt aagttgcgtc gcgccaatga cctcgccggt
    12421 cttaacaatt tgcacattcc cgtcgctcat caagtttttc gcatcagatc ccatgattta
    12481 ttcctttgct gcatctgtgt gcctttattg ctacctaagt gtaaaggcta cggaggattt
    12541 atccacgaca gatttgagat ggtggcaaac aactctgttt aaactctgat acacgaatta
    12601 ttgggttgta tcagatgtaa atgcgatcct gaataaaaat cgcccttgca aatcaacaaa
    12661 atatagccaa tttgcttaaa agctctgcaa acggtcgaaa tagcgcagaa aattacgttt
    12721 tgcctcttgc cacctttcca ctctgccgct aatattcgtc cccgttgtca cctacaacgt
    12781 tgcgttcata gctcagttgg ttagagcacc accttgacat ggtgggggtc gttggttcga
    12841 gtccaattga acgcaccatc ctgcgtccgt agctcagttg gttagagcac caccttgaca
    12901 tggtgggggt cggtggttcg agtccactcg gacgcaccag attttcttaa tctggtcttt
    12961 tcctttttcc ctctgtttct tctctgtatc caatacgtta aaagatttac actgtcttcg
    13021 tctgcgttat cagaaggaga atcgctatgg caactttgtt acaatttcat tttgctttta
    13081 atggcccatt tggcgacgca atggctgagc agcttaaacc acttgctgag tcgattaatc
    13141 aggaacctgg ttttctgtgg aaggtatgga cagaaagtga aaagaaccac gaagccggtg
    13201 ggatctacct tttcactgat gaaaaaagcg cccttgccta tctggaaaaa catactgccc
    13261 gactgaaaaa tctcggcgtt gaggaagttg tcgccaaagt tttcgatgtc aatgagccac
    13321 ttagtcaaat caatcaggca aaactcgcct gacagaattt aatcaagggc ggttagcgcc
    13381 cttttcatcc ctgtctgaaa tttctcaaat tctaaaaatc tcaaccaaac ttatctgata
    13441 acactaaatt cgaaagaatg cgtacaggta agtaacaatg aaaaaaattg ctattgtggg
    13501 tgccgggcct acggggatct acaccttatt ctcgcttcta cagcaacaaa ctccactttc
    13561 tatttctatc ttcgagcagg ctgacgaggc cggtgtcggg atgccataca gtgatgagga
    13621 aaactcaaaa atgatgctgg caaatattgc cagtattgaa ataccgccga ttaattgtac
    13681 gtatctcgaa tggctacaaa agcaagaagc cagccatctc cagcgttatg gcgttaaaaa
    13741 agaaaccttg cacgatcgtc agtttttacc gcgaattctg ctgggcgaat atttccgcga
    13801 tcaattttta cgattagtag accaggcacg acagcaaaaa tttgcagtgg ctgtttatga
    13861 atcatgccag gttaccgatc tgcaaattac aaatgctggc gtcatgctcg ctacaaatca
    13921 ggatttaccc agcgagacgt ttgatttagc ggtgattgcc acgggtcacg tctggcctga
    13981 tgaagaagaa gcaaccagaa cgtattttcc cagcccgtgg tcaggcctga tggaagcaaa
    14041 ggtcgatgcg tgtaacgtgg gtattatggg aacatccttg agcggactgg atgcggcaat
    14101 ggcagtggct attcagcatg gttcgttcat tgaagatgat aaacaacacg tcgtttttca
    14161 ccgcgataac gcaagtgaaa agctaaatat tacgttgatg tcgcgcacgg gtattttacc
    14221 cgaagccgat ttctattgcc ctattccctg cgagccctta cacatcgtca ccgatcaggc
    14281 attaaatgct gagattcaaa aaggtgaaga gggccttttg gatcgggtat ttagattgat
    14341 agtagaggaa atcaagtttg ctgatccaga ctggagccaa cgcatagcct tagagagcct
    14401 gaatgtcgat tcctttgctc aagcctggtt tgccgagcgc aaacaacgcg acccatttga
    14461 ctgggcagaa aaaaatctcc aggaagtcga acgcaataaa cgagaaaaac atactgttcc
    14521 ctggcgttat gtcattctgc gcctgcatga agccgtacag gaaattgttc ctcatctgaa
    14581 tgaacacgac cataaacggt tcagtaaagg ccttgccagg gttttcatcg ataattatgc
    14641 ggcaatccct tcagagtcta ttcgtcgact actggcctta cgtgaagcgg ggatcattca
    14701 tattctcgcc ctcggtgaag actacgaaat ggaaattaat gagtcgcgca ccgtcctgaa
    14761 aacggaagac aacagctact cgtttgacgt ttttattgat gcccgcggac agcgtccgct
    14821 taaagtgaaa gatattcctt tccctgggct acgcgagcaa ttacagaaaa caggggatga
    14881 aatccctgat gttggcgaag attatacgtt acagcaaccc gaagatattc gtggacgcgt
    14941 agcgttcggc gcgttgccct ggctgatgca cgaccagcct ttcgttcagg gacttacggc
    15001 atgtgcagaa attggtgagg cgatggctcg ggcggtcgta aagcctgcat cccgtgctcg
    15061 tcggcgtctt tcgtttgatt aaaggtaaag ctatcagcac tcaacaaact gatgctgttc
    15121 attaagacga tagcggactc cggttcgaat attgttttca ccttcaatgg cgacggcgaa
    15181 gcgaactctt tcgccgtcat gatatgccag tgcggcgctt ccccctagcc cgagaataat
    15241 tgagtcgacc acgcccgtgc tcactaccgt tgaattttca ccgctggcaa tgatgtgtac
    15301 attgtcgccg ctgttggcaa ttctggcgtt tgcgccaaag ctggcgattt gcaccaggtc
    15361 accattactg gcgacatgac accgttcacc caatgtacaa acgcgtactc gcattccggt
    15421 attcgcaatc cgactactat ctccagcgct gcttatccgc gaagaattac cggttacagc
    15481 aacccgtgcg cgttcaccgg agctgccaat gtgactgtta aagccgacac ttccaatccg
    15541 cacgctataa cccatactgg caatttgcgc ggcataccca gcggttgcga ttcgggcgtt
    15601 atcctcacaa cagccgatgc gagcatgatc gtcgctgcgg gcaaactgac tgcaaaatgg
    15661 tatctctccg gggtctgtgg ccagtttttg catagaggaa acttcctgat gcgcgaagtt
    15721 ttcatccgcc agccattttt tccacgcata ctcaaccaga ctctccgccc agtccgtata
    15781 tccttcctca atcagtgcat gacgaatatc ggcataacta ccgccgcgtg ggaaatggcg
    15841 caggaaccag cgatacatca ccgctccgat acgccattct cgcaggtctg ctcgtgtaat
    15901 gatcattcgt tagtgccggt gatagccatc gaccatgctt ttaaaggttt cgtgtggcgt
    15961 acgccccgtg gtgcaaagat aaagatgacc gaagataaaa aagaggctta taaatgccag
    16021 agcaaaatgc gcctgcaata accagtatct tacgccagga aacacatctc ccacggcttg
    16081 cggatagaga cacaacagcc ccgtcaatag taacaacggc agcaatccat acatgacacc
    16141 aacataggcg acctgctgta agggattaaa tttagactgt gttgttgccg ggaaaggatg
    16201 ttcttccccc tgcataatgc caaacaaata aaatcgcgtt tgttttgccg ctcgttccag
    16261 ccacccctga cgacgaatgc gatagtggtg accattatcc ccaacggcat tgatcagcac
    16321 aaagccgagc cagcatgcca gtaacaaaaa tcagcaaact tcatgcaccg caaccagact
    16381 tttaaccgca gttgcgccca ccatcgcaaa gtgatttatc agcccactt
//
LOCUS       DADMEQ010000084        16326 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.085, whole genome shotgun sequence.
ACCESSION   DADMEQ010000084 DADMEQ010000000
VERSION     DADMEQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.085"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(89..2065)
                     /gene="speA"
                     /locus_tag="JNZ05_002755"
     CDS             complement(89..2065)
                     /gene="speA"
                     /locus_tag="JNZ05_002755"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HAY8565928.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENALPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2074..2205)
                     /gene="yqgB"
                     /locus_tag="JNZ05_002756"
     CDS             complement(2074..2205)
                     /gene="yqgB"
                     /locus_tag="JNZ05_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HAY8565929.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2341..2556
                     /locus_tag="JNZ05_002757"
     CDS             2341..2556
                     /locus_tag="JNZ05_002757"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565930.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2860..4014
                     /gene="metK"
                     /locus_tag="JNZ05_002758"
     CDS             2860..4014
                     /gene="metK"
                     /locus_tag="JNZ05_002758"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAY8565931.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4451..5845
                     /locus_tag="JNZ05_002759"
     CDS             4451..5845
                     /locus_tag="JNZ05_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY8565932.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5922..6419
                     /locus_tag="JNZ05_002760"
     CDS             5922..6419
                     /locus_tag="JNZ05_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HAY8565933.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLAWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6514..7221
                     /gene="endA"
                     /locus_tag="JNZ05_002761"
     CDS             6514..7221
                     /gene="endA"
                     /locus_tag="JNZ05_002761"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HAY8565934.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7301..8032
                     /gene="rsmE"
                     /locus_tag="JNZ05_002762"
     CDS             7301..8032
                     /gene="rsmE"
                     /locus_tag="JNZ05_002762"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAY8565935.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8045..8995
                     /gene="gshB"
                     /locus_tag="JNZ05_002763"
     CDS             8045..8995
                     /gene="gshB"
                     /locus_tag="JNZ05_002763"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HAY8565936.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9032..9667
                     /locus_tag="JNZ05_002764"
     CDS             9032..9667
                     /locus_tag="JNZ05_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708713.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HAY8565937.1"
                     /translation="MTAPFFPRILAAVAFLNQETEPLTMNLQHHFLIAMPALQDPIFR
                     RSVVYICEHNTNGAMGIIVNKPLENLKIEGILEKLKITPEPRDESIRLDKPVMLGGPL
                     AEDRGFILHTPPSNFASSIRISDNTVMTTSRDVLETLGTDKQPSDVLVALGYASWEKG
                     QLEQEILDNAWLTAPADLNILFKTPIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9667..10083
                     /gene="ruvX"
                     /locus_tag="JNZ05_002765"
     CDS             9667..10083
                     /gene="ruvX"
                     /locus_tag="JNZ05_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAY8565938.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10259..11239)
                     /locus_tag="JNZ05_002766"
     CDS             complement(10259..11239)
                     /locus_tag="JNZ05_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HAY8565939.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11257..11961
                     /gene="yggS"
                     /locus_tag="JNZ05_002767"
     CDS             11257..11961
                     /gene="yggS"
                     /locus_tag="JNZ05_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HAY8565940.1"
                     /translation="MNDIAHNRAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYIRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11979..12545
                     /gene="yggT"
                     /locus_tag="JNZ05_002768"
     CDS             11979..12545
                     /gene="yggT"
                     /locus_tag="JNZ05_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HAY8565941.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            12542..12832
                     /locus_tag="JNZ05_002769"
     CDS             12542..12832
                     /locus_tag="JNZ05_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HAY8565942.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12840..13433
                     /locus_tag="JNZ05_002770"
     CDS             12840..13433
                     /locus_tag="JNZ05_002770"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HAY8565943.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAEMTREEKSAISHRGQALKLLLDALRNG"
     gene            13426..14562
                     /gene="hemW"
                     /locus_tag="JNZ05_002771"
     CDS             13426..14562
                     /gene="hemW"
                     /locus_tag="JNZ05_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HAY8565944.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRSRLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGMQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14876..15839
                     /locus_tag="JNZ05_002772"
                     /pseudo
     CDS             14876..15839
                     /locus_tag="JNZ05_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839537.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15884..>16326
                     /locus_tag="JNZ05_002773"
     CDS             15884..>16326
                     /locus_tag="JNZ05_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAY8565945.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYA"
BASE COUNT         4042 a         4206 c         4111 g         3967 t
ORIGIN      
        1 aaagcagcta gattattttt aagaatatat aaaaagaaag cgggccgatt tacgtaaata
       61 aatcgacccg ccagcggaat aatcttaatt attcatcttc aagataagta taaccgtaca
      121 aacctgcctc gaactcttca aggaactgtt gttgcagttc agcatcaaga tcggttttct
      181 tcacttgatc gcggaactgg gttaacagcg ttttcggatc gagctgtaca tattgcagca
      241 tgtccgccac ggtatcgcct tcgtcagaca gttctacttc tacgctaccg tcagggaaca
      301 cgaacacgtc aactgcttcg gtatcaccga acaggttgtg catattgcca aggatctcct
      361 gatatgcacc gaccataaag aaaccgagca tcggcggatt ctctggatcg tactccggca
      421 ttggcattgt cgtggcaata ccatcaccat caatatagtg gtcgatagca ccgtcagagt
      481 cacaggtaat atccagcagc acagcgcgac gttccggcac ttgatccagc ccttccagcg
      541 gtagaaccgg gaacaactgg tcgatccccc atgcgtccgg cattgactgg aacagcgaga
      601 aattgacgta cattttgtcc gccatacgtt cctgcagttc gtcgattatc ggacgatgcg
      661 cacggttttg cggatccagt tgcttctgca cttcatggca catgctcaga tagagttgtt
      721 ctgcccatgc acgttcttgc aggctaaagg tgccggaaga gtagccgata tgaatgtcgt
      781 gcagatccat ctggctgtcg tgtaaccatt cacgcaaaga acggcgagtt cccggttcgt
      841 gcatttcctg ccaggtttcc cacatgcttt gcagcgcacg cggcgcatct tctgcgggcg
      901 cggtcggcac cgtatattcg ttacgttcca cgccgatgat attcgacacc agcacggtat
      961 ggtgcgcagt caccgcacgg cccgattcgg tgattaccgt cggatgcggc agagcatttt
     1021 cttcacacgc atcgccaatc gcccagatga tgttgttggc gtattcattg aggccatagt
     1081 ttacggagca gtcagactgc gaacgggtgc cttcataatc cacgcccaga ccgccgccga
     1141 cgtcgaagca ctgaatattg acgcccagct tgtgcagttc tacatagaaa cgcgcggatt
     1201 cacgaacgcc tgtcgcgata tcgcgaatat tcgccatctg cgaaccgagg tggaagtgca
     1261 gtagttgcag gctgtcgaga cgcccggctt cacgcagggt ttcaaccagt tgcagtacct
     1321 gagtcgcagc caggccgaac ttcgattttt ccccgccgga ggactgccat ttacccgagc
     1381 cctgcgaagc cagacgtgca cgcacgccca gacgaggaac gacattcaga cgttctgctt
     1441 catccagcac aatggcgatt tctgacatct tctcaatgac cagatagacc ttgtgcccca
     1501 tcttctcgcc aattaatgcc aggcggatat attcgcggtc tttataaccg ttgcagacga
     1561 tgacgctacg agtcatgcca gcatgtgcca gcactgccat caactcggct ttggaaccgg
     1621 cttccagacc cagcggttca cccgaatgaa tcagggactc gatcacgcgg cggtgctggt
     1681 taactttgat cggataaaca aggaagtaat cgccgttata gccgtaggat tccctcgcac
     1741 gtttgaacgc ggcgttaatg gaacgcaaac ggtgctgcag gatctgtggg aaacagaaca
     1801 gtgcaggcag acgctgaccc tgtgcttcac gagttttcac taactgcgcg agatcgacgc
     1861 gagcttccgg gacgtccggg tccgggcaca cgctgatgtg gcccagctcg ttaacgtcat
     1921 agtagttatt gccccaccag gcaatattgt aagtacgcag catcttgctg gcttcctggg
     1981 agctcattgc aacctcctgc atggagcgta gtacaccgtg ttcgcccgct gacgaaggca
     2041 aacccataga catgtcgtca gacatagcga acctcaaatt attttattaa gtgtaaaaca
     2101 gttaacgact gtcgcagcct ggaactgcga taacaaccca taagcacatg gattttccag
     2161 cagtgtatgc tgacgctccg actgcgcaac cggtttcttt ttcataacat tattaagcac
     2221 ataaccgaac gtaagtgtga cagttcggcg aaaccacgag aaaactcttg tttttacaag
     2281 agcgcccttg ttcagtcctc agtaactgta accagctctt gaatcctgag aagcgccgag
     2341 atgggtataa catcggcagg tatgcaaagc agagatgcag agtgcgggga acgaatcttc
     2401 accagaacgg tgagacaggt taagcagcag acaacggttc attatttcgt atcacctcca
     2461 cggccgcctg ttaagacgaa cccacaagcc aaaactctga tttcaacccg gctggaagtg
     2521 gcaacacgaa aaaaacgtcg tgtgcttttt atttaagccg cgcgccgcgt tttatacccc
     2581 acaatggcag aaaattgcaa aagataaata cgcagaatgc cggcattgtc aggaaaaatt
     2641 tccagccacg tttttaacag aatgagacac gattcaaaaa aaagtggaaa tcgggtgaag
     2701 aattgaccta aaatagccat ccagatgtta atccatccat accgattaac actcagactg
     2761 ccagtgtttt taacctgcag agtcgtggta ggatccgcta ccacagaaaa tccacacaac
     2821 agtttgagct aaccaaattc tctttaggtg atattaaata tggcaaaaca cctttttacg
     2881 tccgagtccg tctctgaagg gcatcctgac aaaattgctg accaaatctc tgatgccgtt
     2941 ttagacgcga tcctcgaaca ggatccgaaa gcacgcgttg cttgcgaaac ctacgtaaaa
     3001 accggcatgg ttttagttgg cggcgaaatc accaccagcg cctgggtaga catcgaagag
     3061 atcacccgta acaccgttcg cgaaattggc tatgtgcatt ccgacatggg ctttgacgct
     3121 aactcctgtg cggttctgag cgctatcggc aaacagtctc ctgacatcaa ccagggcgtt
     3181 gaccgtgccg atccgctgga acagggcgcg ggtgaccagg gtctgatgtt tggctacgca
     3241 actaatgaaa ccgacgtgct gatgccagca cctatcacct atgcacaccg tctggtacag
     3301 cgtcaggctg aagtgcgtaa aaacggcact ctgccgtggc tgcgcccgga cgcgaaaagc
     3361 caggtgactt tccagtatga cgacggcaaa atcgttggta ttgatgctgt cgtgctttcc
     3421 actcagcact ctgaagagat cgaccagaaa tcgctgcaag aagcggtaat ggaagagatc
     3481 atcaagccaa ttctgcccgc tgaatggctg acttctgcca ccaaattctt catcaacccg
     3541 accggtcgtt tcgttatcgg tggcccaatg ggtgactgcg gtctgactgg tcgtaaaatt
     3601 atcgttgata cctacggcgg catggcgcgt cacggtggcg gtgcattctc tggtaaagat
     3661 ccatcaaaag tggaccgttc cgcagcctac gcagcacgtt atgtcgcgaa aaacatcgtt
     3721 gctgctggcc tggccgatcg ttgtgaaatt caggtttcct acgcaatcgg cgtggctgaa
     3781 ccgacctcca tcatggtaga aactttcggt actgagaaag tgccttctga acaactgact
     3841 ctgctggtac gtgagttctt cgacctgcgc ccatacggtc tgattcagat gctggatctg
     3901 ctgcacccga tctacaaaga aaccgcagca tacggtcact ttggtcgtga acatttcccg
     3961 tgggaaaaaa ccgacaaagc gcagctgctg cgcgatgctg ccggtctgaa gtaatctttc
     4021 ttcacctgcg ttcaaaggcc agcctcgcgc tggccttttt cttttacctc accgcggtgg
     4081 ataggcgttc acgccgcatc cagcaaaaaa actgcccgca caataacatc attcttcctg
     4141 atcacgtttc acggcaaatt accatcacaa ttgaaaccga ttacaccaac cacaccagca
     4201 aaatatttgt aatattttca tattattatt cagctttcac agttgttaca tttcttttca
     4261 gtaaagtctt aattgcagat gacagcgttt aatctatgat gatataactc aattattttc
     4321 atgcacttaa atcataacta agataaatgt tagtgtaagc gattacactg atgtgatttg
     4381 cttcacatct ttttacgtcg tactcaccta tcttaattca caataaaaaa taaccatatt
     4441 ggagggcatc atgcctgacg ctaaaaaaca ggggcggtca aacaaggcaa tgacgttttt
     4501 cgtctgcttc cttgccgctc tggcgggatt actctttggc ctggatatcg gtgtaattgc
     4561 tggcgcactg ccgtttattg cagatgaatt ccagataact tcgcacacgc aagaatgggt
     4621 cgtaagctcc atgatgttcg gtgcggcagt cggtgcggtg ggcatcggct ggctctcctt
     4681 taaactcggg cgcaaaaaga gcctgatgat cggcgcaatc ctgtttgttg ccggttcact
     4741 gttctctgcg gctgcgccaa acgttgaagt actgattctt tcgcgcgttc tgctggggct
     4801 ggcggtgggt gtggcctctt ataccgcacc actgtacctg tcagaaattg cgccggaaaa
     4861 aattcgcggc agtatgatct cgatgtatca gttgatgatc actatcggga tcctcggtgc
     4921 ttatctttct gataccgcct tcagctacac cggtgcatgg cgctggatgc tgggtgtgat
     4981 tatcatcccg gcaattttgc tgctgattgg tgtcttcttc ctgccagaca gcccacgttg
     5041 gtttgccgcc aaacgccgtt ttgttgatgc cgaacgcgtg ctgctacgcc tgcgtgacac
     5101 cagcgcggaa gcgaaacgcg aactggatga aatccgtgaa agtttgcagg ttaaacagag
     5161 tggctgggcg ctgtttaaag agaatagcaa cttccgccgc gcggtgttcc ttggcgtact
     5221 gttacaggta atgcagcaat tcaccgggat gaacgtcatc atgtattacg cgccgaaaat
     5281 cttcgaactg gcgggttata ccaacaccac cgagcaaatg tgggggacag tgattgtcgg
     5341 cctgaccaac gtacttgcca cctttatcgc aatcggcctt gttgaccgct ggggacgtaa
     5401 accaacgcta acgctgggct tcctggtgat ggctgctggc atgggcgtac tcggtacaat
     5461 gatgcatatc ggtatccact ctccgtcggc gcagtatttc gccatcgcca tgctgctgat
     5521 gtttattgtc ggttttgcca tgagtgccgg tccgctgatt tgggtactgt gctccgaaat
     5581 tcagccgctg aaaggccgcg attttggcat cacctgctcc accgccacca actggattgc
     5641 caacatgatc gttggcgcaa cgttcctgac catgctcaac acgctgggta acgccaacac
     5701 cttctgggtg tatgcggctc tgaacgtact gtttatcctg ctgacattgt ggctggtacc
     5761 ggaaaccaaa cacgtttcgc tggaacatat tgaacgtaat ctgatgaaag gtcgtaaact
     5821 gcgcgaaatc ggcgctcacg attaatctcc tcaggcttcc tcccgtcgcg gaggaagccg
     5881 cctcttgcag tcatcttttc ttcgctctat cctctgccgc tatgaaaaca tcccgtctcc
     5941 ctatagccat ccaacaggcc gttatgcgcc gcctgcggga aaaactcgcc caggccaacc
     6001 tgaagctagg gcgtaactac ccggagccaa aactctctta cacccagcgc ggaacctccg
     6061 ccggaacagc ctggctggaa agctatgaaa tccgcctcaa tcccgttttg ctgttggaaa
     6121 acagtgaagc ttttattgaa gaagtggtac cgcacgaact ggcacatttg ctggcatgga
     6181 aacatttcgg ccgcgtagcg ccacatggca aagagtggaa gtggatgatg gaaagcgtgc
     6241 tgggcgttcc cgcccgtcgt acgcatcagt tcgaactgca atccgtgcgt cgcaacacct
     6301 tcccctaccg ctgtaagtgc caggaacatc agcttaccgt acgccgccat aatcgcgtag
     6361 ttcgtggcga ggccatctat cgctgtgttc actgcggtga acagctggtt gcgaaataac
     6421 cacctgaact atcaggaact ttcctgatct ggctgattgc ataccaaaac aactttcgct
     6481 acgttgctgg ctcgttttaa cacggagtaa gtgatgtacc gttatttgtc tattgctgcg
     6541 gtggtactga gcgcagcatt ttccggcccg acgttggccg aaggtatcaa tagtttttct
     6601 caggcgaaag ccgcggcggt aaaagtccac gctgatgcgc ccggtacgtt ttattgcgga
     6661 tgtaaaatta actggcaggg caaaaaaggc gttgttgatc tgcaatcgtg cggctatcag
     6721 gtgcgcaaaa atgaaaaccg cgccagccgc gtagagtggg aacacgtcgt tcccgcctgg
     6781 caattcggtc accagcgcca gtgctggcag gacggtggac gtaaaaactg cgctaaagat
     6841 ccggtctatc gcaagatgga aagcgatatg cataacctgc agccgtcagt cggcgaggtg
     6901 aatggcgatc gcggcaattt tatgtacagc cagtggaatg gcggcgaagg ccagtacggt
     6961 caatgcgcca tgaaggtcga tttcaaagaa aaagctgccg aaccaccagc gcgtgcacgc
     7021 ggtgccattg cgcgcaccta cttctatatg cgcgaccaat acaacctgac actctctcgc
     7081 cagcaaacgc agctgttcaa cgcatggaac aagatgtatc cggttaccga ctgggagtgc
     7141 gagcgcgatg aacgcatcgc gaaggtgcag ggcaatcata acccgtatgt gcaacgcgct
     7201 tgccaggcgc gaaagagcta acctacacta gcgggattct ttttgttaac ccctacccca
     7261 cgcgtacaac cgcgtgggga gacgacgcgg atttttaact atgcgtatcc cccgcattta
     7321 tcatcctgaa ccactgacca gccattctca catcgcgctt tgcgaagatg ccgccaacca
     7381 tatcgggcgc gtactgcgca tggggccggg gcaggcgttg caattgtttg acggtagcaa
     7441 ccaggtcttt gacgccgaaa ttaccagcgc cagcaaaaaa agcgtggaag tgaaggtgct
     7501 ggaaggccag atcgacgatc gcgaatctcc gctgcatatt cacctcggtc aggtgatgtc
     7561 gcgtggtgaa aaaatggaat ttactatcca gaaatcgatc gaactcggtg taagcctcat
     7621 tacgccactt ttttctgagc gctgcggcgt taaactggat agtgaacgtc tgaacaagaa
     7681 gcttcagcag tggcagaaga ttgcaattgc tgcctgtgag cagtgtggtc gtaaccgggt
     7741 gccggaaatc cgtccagcga tggatctgga agcctggtgt gcagagcagg atgaaggact
     7801 gaaactgaat cttcacccgc gcgccagtaa cagcatcaat acgttgcctt taccggttga
     7861 acgcgtccgc ctgctgattg gcccggaagg cggtttatcg gcagatgaaa ttgccatgac
     7921 tgcccgctat caatttactg atatcctgtt gggacctcgc gttttgcgta cagagacaac
     7981 tgcgctcacc gccattaccg cgctacaagt acgatttggc gatttgggct aacggagaag
     8041 aataatgatc aagctcggca tcgtgatgga ccccatcgca aacatcaaca tcaagaaaga
     8101 ttccagtttc gctatgttgc tggaagcaca gcgtcgtggt tacgaacttc actatatgga
     8161 gatgggcgat ctgtatctga tcaatggcga agcccgcgcc catacccgca cgctgaacgt
     8221 gaagcagaac tacgaagagt ggttttcgtt cgtcggtgaa caggatctgc cgctggccga
     8281 tctcgatgtg atcctgatgc gtaaagaccc gccgtttgat accgagttta tctacgcgac
     8341 ctatattctg gaacgtgccg aagagaaagg gacgctgatc gttaacaagc cgcagagcct
     8401 gcgcgactgt aacgagaaac tgtttaccgc ctggttctct gacttaacgc cagaaacgct
     8461 ggttacgcgc aataaagcgc agctaaaagc gttctgggag aaacacagcg acatcattct
     8521 taagccgctg gacggtatgg gcggcgcgtc gattttccgc gtgaaagaag gcgatccaaa
     8581 cctcggcgtg attgccgaaa ccctgactga gcatggcact cgctactgca tggcgcaaaa
     8641 ttacctgcca gccattaaag atggcgacaa acgcgtgctg gtggtggatg gcgagccggt
     8701 accgtactgc ctggcgcgta ttccgcaggg gggcgaaacc cgtggcaatc tggctgccgg
     8761 tggtcgcggt gaacctcgtc cgctgacgga aagtgactgg aaaatcgccc gtcagatcgg
     8821 gccgacgctg aaagaaaaag ggctgatttt tgttggtctg gatatcatcg gcgaccgtct
     8881 gactgaaatt aacgtcacca gcccaacctg tattcgtgag attgaagcag agtttccggt
     8941 gtcgatcacc ggaatgttaa tggatgccat cgaagcacgt ttacagcagc agtaacccac
     9001 cttagcgaga aggatctcgt tgagactctg agtgacagcg cccttctttc cacgcatact
     9061 ggccgctgtt gcttttttga accaggaaac agaacctctg acaatgaatt tacagcatca
     9121 ctttcttatt gccatgcctg ctctccagga tccgattttc cgtcgttccg tggtctacat
     9181 ttgcgaacat aataccaatg gtgcaatggg gatcatcgtc aacaagccgc tggaaaatct
     9241 caaaattgaa gggattctgg aaaagctgaa gatcacgccg gagccgcgtg atgaatcaat
     9301 ccgtctggat aaaccggtta tgctcggcgg cccactggct gaagatcgcg ggtttatttt
     9361 gcatactccg ccctccaatt ttgcttccag cattcgcatt tcagacaaca cggtgatgac
     9421 cacttcccgc gatgtgctgg aaacgctcgg caccgataaa caaccgtctg acgtattggt
     9481 ggctctgggc tatgcctcct gggaaaaagg tcaactggaa caagaaattc tcgataacgc
     9541 gtggctaacg gccccggcgg atctgaatat tctgttcaaa acgccgattg ccgaccgctg
     9601 gcgcgaggcg gcaaaactga ttggtgtgga tattctcacc atgcctggtg tggcaggaca
     9661 cgcctgatga gtggaacctt actcgccttc gacttcggca ccaaaagcat tggcgtagcg
     9721 gtcggccaac gcattaccgg caccgctcgc cctttgcctg caattaaagc gcaggacggt
     9781 acgccggact ggaacctcat cgaacgttta ctgaaagagt ggcagcctga cgaaatcatc
     9841 gtcggtttgc cgctgaatat ggacggcacc gaacagccgt taaccgccag agcgcgcaaa
     9901 tttgccaacc gtattcatgg tcgtttcggt gttgaagtaa agctccatga cgagcgtctt
     9961 agcactgtgg aagcccgttc cggtcttttt gaacagggcg gctatcgggc gctcaacaaa
    10021 ggcaaagttg actctgcctc tgcggttatt attctcgaaa gctatttcga gcagggatat
    10081 taaggcgatc taaaagcctg gcggatagtc gttaccgcca tcttgccgga tgcggcgtga
    10141 acgccttatc cagcacacat ctggcagcgg ctgtaggtct gataagacgc gttagcgtcg
    10201 catcagacat ttattgcctt tataggccct ataaacttgc gcatcgggca tggcaacgtc
    10261 acaaacgccc ttcccccacc cgctgctgat aactctgctg aaacgttaac atccccacct
    10321 gctgcccggt ttgaataaca tgcggtaact ggtgggtttt cccttcgcga atcaaattcc
    10381 ccaccgcggg agtgttaatc agtaattcaa acagcgccac gcgtccttcc tgtttatcca
    10441 cttccagctt ttgtgacagc actgcccgta aactacccgc aagttgatta cgtacgggat
    10501 ctttttcctg cgccggaaat gaatccacca gtcgctcaac tgcctgcgcg gcaccacgtg
    10561 tatgtaaggt tgccagcacc agatgcccgg tttctgccgc cgtcagcgcc agacggattg
    10621 tctcactgtc acgtagctct ccgagcaaaa tcacatcagg atcttcccgc aatgcagccc
    10681 gcaaccccga tgcgaacgtc atacagtgca aaccaatttc ccgctgctgg atcaaacatc
    10741 gctggctggc atagagatat tccacaggat cttccagcgt cagaatatgc gcatcggcat
    10801 gttgattaag atagccaacc atcgccgcca gcgtggtaga tttgccactc cccgtagccc
    10861 ccgtcaccag aatcaggcca ttctcgctct tgagtaattc cggcaatacc ggtggcgcac
    10921 caagctgttc gagctgtgga cagtgcgaag gtaacaaccg taatgccaga gaaatacctt
    10981 gccgttgcgc gaacgcactg ccacgcaacc gctggttttc cgccagcgac acggcaaaat
    11041 ccagctggcc attttccagc aatattgccc gctgatcgtc atccagccac tcccgcagta
    11101 gctcttcgac gtccggcgca tcaaacggcg cagcttccat tctgccacga atgcgccatc
    11161 gtgcgggcca ggcgctgcac aggtgtagat ccgagacgtt atgctttaca ctaagggcca
    11221 caatttcttc catattcata ctaagatcct cggaaaatga acgatattgc gcataaccgg
    11281 gcacaggtcc gggacaaaat ctcagcggct gcaacgcgtt gcggccgttc tccagaagaa
    11341 attacgctgc ttgcagtcag taaaacaaaa cctgcgagcg ccatcgcaga agccattgat
    11401 gccgggcagc gtcaatttgg tgaaaactac gttcaggaag gggtagataa aattcgccac
    11461 tttcaggaac tgggcgtaac aggattagaa tggcatttta ttggcccgtt gcagtctaat
    11521 aaaagccgcc tagtggcaga gcatttcgac tggtgtcata ccatcgaccg tttgcgcatt
    11581 gctacccgcc tcaacgatca gcgcccggca gaacttcccc ctcttaacgt actgattcaa
    11641 attaatatta gtgatgaaaa cagtaagtcc gggattcaac tggcagaact ggacgagctg
    11701 gcagcggcgg tcgctgaact accgcgttta cgtctgcgcg ggctgatggc aatccctgcg
    11761 cctgagtcag aatatataag gcagtttgaa gttgcacgcc aaatggctgt agcatttgcc
    11821 ggactgaaaa cgcgctaccc gcatatcgac acgctctctc tgggaatgtc ggacgatatg
    11881 gaagccgcca ttgcggcagg tagcacgatg gttcgtatcg gcactgcaat ttttggtgcg
    11941 cgtgattact ctaaaaaata aggaattaaa ggaacgccat gaatacgttg actttcctgc
    12001 tttcaacggt cattgagctg tataccatgg tgctgttatt acgcatctgg atgcagtggg
    12061 ctcattgtga tttttacaac cccttctcac agtttgtagt gaaggtagcg cagccaatta
    12121 tcgggccact gcgccgcgtt attccggcaa tgggtcccat tgacagcgcc tcgctgctgg
    12181 ttgcctatat tctcagtttt atcaaagcca ttgtgctgtt tgaagtggtg accttcctgc
    12241 caatcatctg gattgccggt ttactgattc tgctgaaaac catcggcttg ctgattttct
    12301 gggtcctgct ggtgatggcg attatgagct gggtaagcca gggtcgtagc ccgattgaat
    12361 acgtgctgat tcagctggcc gatccgctgc tgcgcccgat tcgccgcttg ctaccggcaa
    12421 tgggtgggat tgatttctcg ccgatgatcc tcgttctgct gctgtatgtc atcaatatgg
    12481 gtgtcgcaga agtattacag gcaaccggaa acatgctgct gccggggctg tggatggcgt
    12541 tatgagtgcc gtaacagtta atgatgacgg tctggtttta cggctctata ttcagccgaa
    12601 agccagccgt gattctattg tcggtttaca tggcgacgaa gttaaagtcg ccattaccgc
    12661 gccgccggtt gacggccagg ccaacagtca tctggtgaag tttctcggta agcaattccg
    12721 ggttgccaaa agccaggtgg tgattgaaaa aggcgaactt ggccgccaca aacaaattaa
    12781 aatcattaat ccgcaacaaa tcccgccaga aatcgcggcg ttaattaatt aggtatccta
    12841 tgcaaaaagt tgtcctcgca accggcaatg ccggtaaagt gcgtgagctg gcgtcgctgc
    12901 ttagcgactt tggtcttgat atcgtggccc aaacagacct cggtgttgat tccgctgaag
    12961 aaaccggcct aacctttatc gaaaacgcga ttctgaaagc gcgccatgcg gcaaaagtga
    13021 ccggtttacc ggcaattgcc gacgactctg gtctggcggt agatgcgctg ggtggcgcgc
    13081 cgggtattta ctccgcgcgt tattccggtg aagacgcgac cgatcaaaag aatctgcaaa
    13141 aactgttgga aacactgaaa gacgtaccgg acgaccaacg tcaggcgcgt ttccactgcg
    13201 tgctggtata tctgcgtcac gcggaagatc cgactccgct ggtatgccac ggcagctggc
    13261 cgggcgtgat tactcgcgaa ccagcgggca ctggtggctt tggttatgat ccaatcttct
    13321 tcgtaccttc cgaagggaaa accgccgccg aaatgacccg cgaagaaaag agcgccattt
    13381 cccaccgtgg tcaggcgttg aaactgctgc tggacgcttt acgtaatggt taaattaccg
    13441 ccgctgagtc tctacattca catcccgtgg tgcgtgcaga aatgcccgta ctgcgatttc
    13501 aactctcacg cgttgaaagg agaagtgccg cacgacgatt atgttcagca tctgctttgc
    13561 gatctggaca atgatgtggc ttacgctcag ggccgtgaag taaagacaat ctttattggc
    13621 ggtggtacgc cgagcctgct ttccggcccg gcgatgcaaa cgctgctaga cggcgtgcgt
    13681 tcgcgtttgc cgctggcagc ggatgcagaa attactatgg aagcgaaccc aggcacggta
    13741 gaagcggatc gctttgtcga ttatcagcgt gctggtgtaa accgcatctc tattggtatg
    13801 cagagtttta gcgaagaaaa gctgaaacga cttgggcgta ttcatggccc gcaagaagcg
    13861 aaacgagcgg caaagctggc gagcggttta gggttacgta gctttaacct tgatttgatg
    13921 catggactac cggatcaatc actggaagag gcgcttggcg atctacgcca ggccattgaa
    13981 ctgaatccgc cgcatctttc ctggtatcaa ctgaccatcg aacctaatac gctgtttggc
    14041 tcgcgaccgc cggtgctgcc ggacgatgac gcgctgtggg atattttcga acaggggcat
    14101 cagttattaa ccgcagcggg ttatcagcaa tatgaaactt ccgcttacgc caaacccggt
    14161 tatcagtgcc agcacaatct caactactgg cgctttggtg actacatcgg tattggctgc
    14221 ggcgcgcacg gcaaagtgac cttcccggat gggcgcattc tgcgtaccac taaaacgcgt
    14281 catcctcgtg gttttatgca aggaaggtat ctggaaagcc agcgtgatgt cgaagccgca
    14341 gataagccgt ttgagttctt tatgaatcgc ttccgtctgc tggaggccgc gccgcgcgtg
    14401 gagtttattg cgtataccgg gctttgcgaa gatgtgattc gcccacagtt agacgaggcg
    14461 attgcccagg gttatctcac cgaatgtgcg gattactggc agataacgga acatggtaaa
    14521 ttattcttaa actcacttct tgaattattc ctcgctgagt aatcattaat gcgcctgcgg
    14581 gcgcattaat cacttctttt atccccccgt taattaacct cggcagaaat atatttctta
    14641 ctgaataaat atcgacgaca tttattatat atatttagtg aatatttact aatcattcat
    14701 tgattataaa tatgtacaac ctggcgcaca aatttaatat ttaatttctg actttatttc
    14761 aagttgtgat aaacatagac aagttgtcag acaggttaac caatttgtcg cacaaagcac
    14821 tgataagcac aattagatat caaccaattt ccatccttca acagggagtt tttttatgaa
    14881 aatgaggtca tttacccgct cattagtctg cgcatccttg ctggctctgg ttagcactgg
    14941 cgtaaacgcc gcagaaaaag tgacgttaaa gttagcacac aacctggaaa gaagtcatgt
    15001 tgtacatcag agttttgaag agctggcgaa agaggtaaaa cagctgtcaa aaggtaatat
    15061 ggtgatccgt atttatccca gcagccagat gggtaatgcg cgagaaacta tggaattgtt
    15121 gcaaaatggc gcactggata tgaccaaagg ttccgccagt gatctggagt cttttgataa
    15181 tatttatgct atctataatt tgccgttctt attaagttgt ttttggtgaa gttggtaaag
    15241 aaattatgga ctcaacaaaa ggcaaaggct tttttgcttt atcagcttat gtagcgggta
    15301 ctcgcagttt ctatgcgaaa aaaccgatta caaagccaga agatcttaaa ggactgaaaa
    15361 ttcgtgttca gccaagtccg accaccatca aaatgattga actgatgggt ggttcaccca
    15421 ctccaatttc atttggcgaa gtttatactg cgatgcagca aggtgtagta gatggcgcag
    15481 aaaataacgt gccttcctgg atgcagactc gacatattga aattgccaaa gttttttctg
    15541 aagatgaaca cgcctcaatt cctgactttt tggttatctc caccaaaaca tggaataaat
    15601 tacccccaga acagcaacaa atcctcgaaa cagcggcgaa gaaatccgaa gcttatcaac
    15661 aaaaattgtg ggaaaaaatt gacgctgaca cacgtgctca ggcgaaagct atggggggtg
    15721 aaattgtaaa agtggataaa gcgccattcc gtaaagccgt gcaacctctg tttgacgact
    15781 tcaaaaaaga tccaaaacaa gctgctttgt tagagaaatt cgacaacgct gctcaataat
    15841 attccgggcc agcctctggc cctgattttc caggatgctt acaatgatat ttaaccgcct
    15901 gaaacttgcg gtagaccgcg tcattgccgc gttttctgtc gccgtaatgc ttgctctggt
    15961 tgtatgcgta gtctggcaag tatttagccg ctacgtactt aaccagccaa gtacattaac
    16021 cgatgagctg gcacgctttt tgatgatctg ggtaggatta ttaggtgctg catataccgt
    16081 cggcgcgcag cgacatctct ctattgattt atttgctctc gccctgaata agcgcaaaca
    16141 attacttctt agtatagtca ttaacgttct cattttaggt tttgccggtt cggtcattgt
    16201 gactggtggc ctgaaattaa ttgataaaac actggcaaca tcacaggtat ctgccgctat
    16261 gcaaataccg atgggatacg tatatatcat tctgccatta agtggagtgg taatgatgtt
    16321 ttatgc
//
LOCUS       DADMEQ010000085        16030 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.086, whole genome shotgun sequence.
ACCESSION   DADMEQ010000085 DADMEQ010000000
VERSION     DADMEQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.086"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(186..872)
                     /gene="kptA"
                     /locus_tag="JNZ05_002774"
     CDS             complement(186..872)
                     /gene="kptA"
                     /locus_tag="JNZ05_002774"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HAY8565946.1"
                     /translation="MWKRFCAGLSCRKFINPVTMTLVNKNRKSRDENAMAKYNEKELA
                     DTSKFLSFVLRHKPEAIGIVLDREGWADINKLILCAQKAGKRLTRALLDTVVATSDKK
                     RFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQFLYHGTASRFLDEIKKQGLIAGDRH
                     YVHLSADEATARKVGARHGSPVVLTVKAQEMAKRGIPFWQAENGVWLTSTVAVEFLEW
                     SFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="JNZ05_002775"
     CDS             1020..1703
                     /locus_tag="JNZ05_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HAY8565947.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="JNZ05_002776"
     CDS             1700..2161
                     /locus_tag="JNZ05_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565948.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="JNZ05_002777"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="JNZ05_002777"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY8565949.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHLESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIL
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="JNZ05_002778"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="JNZ05_002778"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAY8565950.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSRAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSAFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="JNZ05_002779"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="JNZ05_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HAY8565951.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /locus_tag="JNZ05_002780"
     CDS             complement(4704..4787)
                     /locus_tag="JNZ05_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide"
                     /protein_id="HAY8565952.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            5380..6210
                     /locus_tag="JNZ05_002781"
     CDS             5380..6210
                     /locus_tag="JNZ05_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY8565953.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(6351..7124)
                     /gene="uxuR"
                     /locus_tag="JNZ05_002782"
     CDS             complement(6351..7124)
                     /gene="uxuR"
                     /locus_tag="JNZ05_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HAY8565954.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(7339..8799)
                     /locus_tag="JNZ05_002783"
     CDS             complement(7339..8799)
                     /locus_tag="JNZ05_002783"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HAY8565955.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGSAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(8880..10064)
                     /gene="uxuA"
                     /locus_tag="JNZ05_002784"
     CDS             complement(8880..10064)
                     /gene="uxuA"
                     /locus_tag="JNZ05_002784"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAY8565956.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            10404..11747
                     /gene="gntP"
                     /locus_tag="JNZ05_002785"
     CDS             10404..11747
                     /gene="gntP"
                     /locus_tag="JNZ05_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HAY8565957.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(11998..12900)
                     /gene="fimH"
                     /locus_tag="JNZ05_002786"
                     /pseudo
     CDS             complement(11998..12900)
                     /gene="fimH"
                     /locus_tag="JNZ05_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
     gene            complement(12920..13423)
                     /gene="fimG"
                     /locus_tag="JNZ05_002787"
     CDS             complement(12920..13423)
                     /gene="fimG"
                     /locus_tag="JNZ05_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HAY8565958.1"
                     /translation="MKWCKRGYVLAAMLALASATIQPADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(13436..13966)
                     /gene="fimF"
                     /locus_tag="JNZ05_002788"
     CDS             complement(13436..13966)
                     /gene="fimF"
                     /locus_tag="JNZ05_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HAY8565959.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(13976..>16030)
                     /gene="fimD"
                     /locus_tag="JNZ05_002789"
     CDS             complement(13976..>16030)
                     /gene="fimD"
                     /locus_tag="JNZ05_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein FimD"
                     /protein_id="HAY8565960.1"
                     /translation="SVQNRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSG
                     SKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRG
                     FAPVIHGIAHGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGS
                     TQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYG
                     GTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNES
                     GTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGK
                     LQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAW
                     QKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLED
                     NNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVL
                     AHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVAL
                     DTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSE
                     SSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAE
                     CR"
BASE COUNT         3865 a         4130 c         3923 g         4112 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagcc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tgactgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct ttttttccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc tggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattct
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccgg gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgaatccg tgcactggaa caggcgattg gagttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggg gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagcg
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtaag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaac cgctagtata ttctgaatat tccgaatagt tacgtagatg
     4981 aaaatacttg tcaccgctgc tacttaattc cggtgggaaa ttatttattt ccgcataatc
     5041 agctatgtat tatttgaaac aattagtttg taaataatat aaattgataa catatcaaat
     5101 acatcaaaaa taccattcac aggcattatt aatatagacc tcaatatagc aacatcaaat
     5161 tccaattagt aataagatat gcttttaaat tgcatgcaaa taattttaaa tgagcgtttt
     5221 ttgatacttt agagtgtttc ctacccttta atatcactgt gtagagttga tatcactaca
     5281 acagaaacat gcatgtcatt tgtttacttc tctttttact acagaaaaat ttatctattt
     5341 ttctaaacat cagttaaata ctcataaaag gtaactatta tgtctatgcc attaagcaat
     5401 gccgctaata accaattcaa tacaaataat cacttccttc atcatccaaa agtcgacagt
     5461 gagttaacac gtcgatatga atatgcacgc ctggatacgg aaaatatcta tcttctgcct
     5521 cttgctcgtg gcaataacca taattacgat ggaaaaagcg ttgtcgaaat tagaaaactt
     5581 aatattggaa aacagtcctg gccatttaat tatgtgacag atacatgcag agaattcaat
     5641 ggtattacaa caacaggacg tatgctctat cgaaacttga aaatcacctc agctttagac
     5701 gaaatatatg gtggtatatg taaaaaggcg catgctgcta cagagttagc agaaggttta
     5761 cggctaaatt tatttatgaa gagccctttc gatcctgttg aggactatac agttcatgaa
     5821 ataacattag gccctggttg caatgttcct ggttatgccg gaaccacgat aggatatatt
     5881 tcaacccttc cagcttctca ggcaaaaaga tggaccaatg agcagccaag aattgatatc
     5941 tatattgatc agataataac tgtctcaggg gtagcaaatt cttccggatt cgcccttgcc
     6001 gcattactta atgctaatat tgagatggga aatgacccaa tcattggtat agaagcctat
     6061 cctggtaccg cggagatcca ttcaaaaatg ggatataacg ttatccccgg tgatgaagat
     6121 gcgccactca aacgcatgac tctgcaaccg tcatctcttc cagaactgtt tgaattaaaa
     6181 aatggtgaat ggaattatat tggtaaatag ccactgagaa gaatgagtac taatattcag
     6241 gttatacaga tatttaaaca aaaccctgca atttacaaag taatactcat tgcgacttct
     6301 taatttaaga aagcgcaagg aacgtttacc cttgcgctta ttataataag tcaggcgtcg
     6361 actttatcca gcggccatga atcaaagaga tagccgtcaa aataaatatc gtcaacgttc
     6421 gagaattcca gcagacgttg cttaacgttt tccagatgct gccacattgc cagcttcgcc
     6481 gctcgggcat cttttttgat taacgcggcg aggatctgtt tgtgatcgcc caaccactct
     6541 ttgcgataca ggctgtcatc cagatggctg tgcaactgga tccacattgg attgttttcc
     6601 cgccactgcc aggactgacg gaataactcc accagcatgc tgttatgcgt tgcttcagca
     6661 atggcgagat ggaactgcat gtcaccgctt tcgctgctac ccggcgcgct ggaagccagt
     6721 tcacgctctt ccagttgcaa tgcctgacgc attttgacga tatcttcgcg ggtagcctgc
     6781 agtgcggcaa actcggcgat gttactttcc agtaactgac gcgcctgtaa cagctcaaaa
     6841 ggacccgcat cattgcagac gttggcatcc ggactgtctg tgttgtggct gcctgagcta
     6901 tcaagcacat agataccggc accccggcgc acttccacca gccctttgat ctccagcatg
     6961 atcagcgctt cacgtaccac cgtccgcgtg acatcaagca tttctgcaat ttcacgctcc
     7021 ggcggcagcc gttcgccagg attgtacggc gtctttatga tcagatcgcg gatcatcgcc
     7081 ccgacttcct ggtaaggtct ttgcgcagag gtgacagatt tcatggtgtt gtgtccgtta
     7141 ggtgcggtta atcaaatttt tgccggatgc ggcgtgaacg ccttatccgg cctacaacgg
     7201 gcaattttac ggcccccgtt aatctcaaac tataccatta gcactggaag ttggcacgta
     7261 caggtgggca aaaaaacgaa aactggtcaa ccaaatatca gtttgattga ccagtaagtc
     7321 tgtagggtaa tcagttagtt acagcgcagc cacacactcg cgcgcaccgc gttcgcacag
     7381 ctgctgatat gccgccgtca ccgcgccaac aaagtcggca ttctgcggca gatcatcggc
     7441 aaaaataccg ctcaggccca gcagcgcttt cacgcggtct gcgccctgat actgcgcgtt
     7501 aatcttctgg aactcggcca gcatcgggtc gaccacgtca atggcactac cctgctcatc
     7561 cacgccctgg gtgtaacgca tccagccagc cacgcccagc gccaggtgac gccagctgcc
     7621 gccgttttgc aggtgcagac gcaccgggtc cagtagacgc tgcggtaact tctggctgcc
     7681 gtccatcgca atctgccagg tacggtgacg cagagacggg ttgctgaaac gctcgatcag
     7741 cagcgtcgca taggcgttca ggtccgtacc ttccggcatc gacagcgttg gcgcttgttc
     7801 ctgcatcatc agggcaaagg ccgctttgcg ataatccggg ttagtcatgg tgtcggcaat
     7861 ggtttcatag ccgccgaggt aaccgaggta cgccaggaaa gagtggctgc cgttcagcat
     7921 acgcagcttc atcatttcga acggcacaac gtctgcaacg aactgtgcgc ccactttatc
     7981 ccagtccggg cgaccattaa cgaagttatc ttcaatcacc cactgacgga acggttcgca
     8041 ggcaatggcg cacgggtcgt aaacacccag ctggtcagca atttcctgta aggtttctgg
     8101 cgtcgccgcc ggaacgatgc ggtcaaccat ggtgcacggg aaggtgacgt tctcttcaat
     8161 ccatgccgct agctgcgggt cacgcgcctg agccagcccc agtaccgcga ccttcgccac
     8221 atgaccgttt tcacgcacgt tatcgcagga catcaccgta aatgctttca gccctttttc
     8281 acgacgcaga cgcaaggctt cgacgatgta accaatcgcg gacttcggcg cagtcgggtt
     8341 ttccagatcg tgcttgatca gcgggttatt gagatccagt tgaccgcttg ccgcatcagc
     8401 gcagtagcct ttttccgtga ccgttagaga gacaatcgcc gtttgcggac gcgccatcgc
     8461 gttgagaata ccttcgcagc cgtcgatttc cggatgcagc gcttctttca tcgaaccgat
     8521 aattttcagc tcggtgcttt ctgcgccttt ttccgctacg gtgtacagca gttgctgttt
     8581 tttcaggttt tcgatcagca tgcggtcgtt gcctggcatc aggttaactt cgcagatgcc
     8641 ccagtcgctg tcggtgcttt ccagcagatg atgggtatac agcgcctggt gcgcgcggtg
     8701 aaacgccccg caaccgagat gcacaatgcg tgattccaga cgagaatgat cccatgacgg
     8761 gcgggcaacc ggcagattgc tgtcaacaat agtagtcatc gtaaactcca gaatccgtct
     8821 tcagagagtg acggcaagga agaaggaacc ggcacggcgc gcagccatgc cggtgggtat
     8881 taacggctaa agaaagcgcg ctggatcgcc agttcgacac cgcgaacttc ggccaggcct
     8941 ttcagacgac caattgcgga gtaaccaggg ttggttttct tcttcaggtc gtccagcatc
     9001 tgatgaccgt ggtccggacg catcgggatc aggtcttctt tgccttccgc tttacgacgg
     9061 tgttcttctt caacaatcgc tttcaccact tcgtacatat caacgtcacc gttcaggtgc
     9121 gccgcttcgt ggaaggtttt cgggttatct tcacgcatgg tggagcgcag atgggtgaag
     9181 taaatacgcg gaccgaactg cttgatcata tcaaccagat cgttgtcagc acgcacgccg
     9241 taggaaccgg tgcacatggt gaaaccgttc gccatgctgt ttacggtatc aaccatccac
     9301 tgcatatctt caatggtgga aacaatgcgc ggcaagccga ggatcgggcg cggcggatcg
     9361 tccgggtgaa ccgccatacg cacgccaact tcttcagcaa ctggaataat cgctttcagg
     9421 aagacggcaa agttttcgcg cagtttggct ttgtcgatat ctttgtacag ctccaggtgc
     9481 ttacggaact ggtcgagggt atacccttct tccgcgcccg gcagaccggc aatgatgtta
     9541 cgcgtcagac gcgctttgtc ttcgtcgctc atagtggcga agcgttcagc tgcctgagca
     9601 atttcttctt cggtgtaatc cgcttccgcg cctggacgtt tcaggatatg catttcgaat
     9661 gcagcgaatt cgatctggtc gaagcgcaga gctttggagc cgtctggcag cacgtattcg
     9721 aggtcagtac gggtccagtc gagcaccggc atgaagttgt agcacacggt gcgaatgcca
     9781 cactgcgcca ggttgcgcag agtctgctga tagttagcga tccactgttc atagttgcca
     9841 gtgtgggttt tgatatcttc gtgaattggt acgctttcta cgacagacca cactaggcct
     9901 gcgtcttcaa cgatcgcctt gcgtttgagg atctcttcta cggaccatac ttcgccgttc
     9961 gggatatggt gcagcgcggt aaccacgcca gttgcgcccg cctgacggac atcagctaaa
    10021 gaaaccggat cgtttgggcc gtaccagcgc caggtctgtt ccatgatttt tcctctttca
    10081 catagtgggt tccacagcaa agagacgcgg aaccttggag ttgtttaggt atccggatga
    10141 acaaccagac cgcagccgtg gcgttgcatc ggatgagatg gcgtataagt tctacctttt
    10201 ggtaagacat gtcaacccga actctgaaat tggttaacca catcacaaga atttcactct
    10261 catggttgtt gctgcaaatt gcagcaaagg gtaatcgatg gttattgatc agaggatgtg
    10321 acattcatcg caacaatggt tgaccaattt gcataacatg tcgcccaaaa taacaacggt
    10381 tcaaccaagt aaggtaaaac gttatgcatg tgcttaacat tctctgggtg gtattcggca
    10441 ttggcctgat gctgatactg aatttgaagt tcaaaatcaa ttcaatggtg gctttgttgg
    10501 tggcggcgct gtccgtcggg atgctggcag gcatggattt gatgtcgctg ctgcacacca
    10561 tgaaagcggg cttcggcaac acgctgggag aactggctat catcgtggtg ttcggtgcgg
    10621 tcatcggtaa attgatggtc gactccggcg cggctcacca gatagcacat acgctgctgg
    10681 cgcgtctagg tctgcgctat gttcagctgt cggtgattat catcggcctt attttcggtc
    10741 tggcgatgtt ctatgaagtg gcctttatca tgttagcgcc gctggttatt gttattgccg
    10801 ccgaagctaa aattccgttc ctgaaactgg cgatcccggc agtagcagct gccactaccg
    10861 cacattcact gttcccaccg cagccgggtc cggtggcgct ggtgaatgct tatggcgcgg
    10921 atatggggat ggtttatatc tatggcgtac tggtgacgat cccaagtgta atctgcgcag
    10981 gtctgatcct gccgaagttc ctcggcaatc ttgagcgccc aacgccatca ttcctgaaag
    11041 cagatcaacc ggtagatatg aataatctgc cctctttcgg cgtttcgatt ctggtgccgc
    11101 tgatcccggc gatcattatg atctctacca ccatcgccaa tatctggctg gtaaaagata
    11161 cccctgcctg ggaagtggtt aactttattg gttcctcgcc gattgcgatg tttattgcga
    11221 tggtggttgc attcgtactc tttggcaccg cgcgtggtca tgacatgcag tgggtgatga
    11281 acgcttttga aagcgcggtg aagagtattg caatggtgat tctgatcatc ggtgcgggtg
    11341 gcgtgctgaa gcagaccatc atcgacaccg gcattggcga caccatcggc atgttgatgt
    11401 cccacggcaa tatctcgccc tacattatgg catggctgat cactgtgcta attcgtctgg
    11461 cgacgggtca gggtgtcgtt tcggcgatga ccgccgccgg gattatcagt gctgcaatcc
    11521 ttgatccagc aaccggtcag ctggttggcg tgaatccggc gctgctggta ctggcgacgg
    11581 ctgcgggttc caacaccctc acccacatta atgatgcatc tttctggttg ttcaaaggtt
    11641 actttgacct gtcagtaaaa gacacgttga aaacctgggg tctgctggag ctggtcaact
    11701 ccgtggttgg gctgattatt gtgctgatta ttagcatggt agcgtaaaag aacaccagag
    11761 cctgccaatg gcaggctcag actgatgact aaagtctaaa aatgtgcccg gacagtcccc
    11821 tcgcccctcc ggggagaggg ttagggtgag gggaacagac cagtaaagtg cgctttgttt
    11881 atgccagatg cgacgctgac gcgtcttatc tggcctacaa agggctaacg tgcaggtttt
    11941 gtaggtctga tagcgtagcg cctcaggtac cagcattagc aatgcgctgt aattttttta
    12001 ttgataaaca aaagtcacgc caataatcga ttgcacattc cctgcagtca cctgccctcc
    12061 ggtacgtgcg taatttgccg ttaatcccag acttaccgcc gaagttccta ctgctcctaa
    12121 cgataccgtg ttattcgttg gaataatcgt accgttgcgc gtcaactgta cgccgacgcc
    12181 ctgcgctggt gaaaacgacg cggtattggt gaaaatcgag ttgcccgcat ctgcggttgt
    12241 gccggagagg taatacccca ggttttggct tttcgcacaa taaacggtaa gaggaattgg
    12301 cactgaacca gggtagtccg gcagagtaac ggtgacatca cgagcagaaa catcacaacc
    12361 gccagtgggc accaccacat cattattggc gtaaatattc cacacaaact gaaaatcatc
    12421 gctgttatag ttgttggtct gtcgcaaaat aagcacggca attaatgaac cagctttaat
    12481 cgccaccccg cccgcactgc tcacaggcgt caaataaagc gccaccggct acggcttatc
    12541 cgttctcgaa ttataaacaa cccgcggcgt ttcgctggtg gtcggaaatg gatagctact
    12601 gccactatat tttacggtcc cggaaaaatt agataacacg ccgccgtaag ccgagcctcg
    12661 ttgcagtgtg acatagtctg taatggtttc cggataatcg ttatggcaaa agatttgcgt
    12721 cgaaagatct acgaccaggt tttgccccac attcacgaca ggcgcaaggt ttacataaac
    12781 attagcgctg ccaccgccaa tagggatagc ggtaccattg gcggttttac aggcgaatga
    12841 ccaggcattt accgaccagc ccatcagcag tacagcaaac agggtaatag ctcgtttcat
    12901 tacaattatc tcttcgggtt cagctgtagg tataggtgat gctaatcact gcctgaatgg
    12961 ttccctgagt ggctccgcca tttaccgtca atgctctgac ctgtaacggg aagtgcgctg
    13021 attgtgagga atcatccacc tgaactgttt tggttgcgcc agtattcaat gtgttgccac
    13081 tgtcatcctg tagctctaac tggatgtttt gcgcggtccc ctggttttta taatatccgg
    13141 tgctgtcggc tgccccgctg aagctggcag tgaccctcga cgttcccacc ggacaattag
    13201 tcaactcaag cgcaacatca tgccaggccg atgccgcccc ggcagacata agactgaaag
    13261 aataaagatc gccgagatct accgtggcat tggtggtgga aaccgtacac ggtttggcga
    13321 cgaccttacc gttcaccgtg atggtgacat cggctggctg tatcgtcgca cttgcgagcg
    13381 ccaacattgc cgccaataca tacccacgtt tgcaccattt catgagcatc tccagttact
    13441 gatattcaag agtgaaggta gccgtagcat tgatatgccc cgcagtgaca ggcacctgtg
    13501 tcgccattag ccgggcgtaa aaattcagcg tatttggttt acccggcgtc agggtcgtcc
    13561 acgaaatcgc agacgatgga gcattaaggg gtatttgatt ttgctgctca ttcagaagct
    13621 gtattcccag tcccgcagcc gctgacaccg tattttcaag tgcaagcagg ttggcattgt
    13681 ggctatctgc aacgccggta aacccaactt ttacggcaga aacggcatta ccacagggtg
    13741 acagcaaaat acgaaatgga acgacaggag tcgtcgcgcc aatgttgtta aattgcttcg
    13801 ccgcgttttc catcagatca acagtaaaat tggttgattc agcggccaca ctacagccgt
    13861 tatctctgac atagccgcgg atagtaatcg tgctatccgc agccaaaacg cgacttaccg
    13921 ccagccacaa aaaagcgcac agaagataaa aaggtttgtt tctcatcacg cccccttaac
    13981 gacattcagc tgatagctgg gttaataact gctgctgact ctctggtggc agttgataat
    14041 tggcgacaca gtgagcattt tcctcttctc cccatttcac ctgaactttt cccgctaaag
    14101 gcattccgct gaggtaaacc tgaccattat ccgcaacaat gccgctactc tggctactct
    14161 ctgatgtcac catcgcccca aacggcagcg gcttattatt gtgagttagc gtcatgagca
    14221 gttttatccc aacgcgcgct ttaaactctg ctcgcacgat cgccccacga gtgggaacaa
    14281 cgttagcgac cgcgttatct aaatcgacgt tatcagccaa ggtattggta tccagcgcca
    14341 ctctattttc ccgatattca gtggcataag gcagcacggc ataaccacgc cagtcggtac
    14401 gcacccccgt ctggttttcg acttttgcat cttttgcgcc aggcgcttta acaagcacca
    14461 ccgtatcgtt taacggctgc cccagcgtta cgccattggc atgagccaat accccaccgc
    14521 tgactccgta atagagctgc ttaatatcat cgctatggct gtaaccgata ttggcattgc
    14581 cgtaaccacc gcgataattc agcgtggcgt agcctgtact tccgctatta ccatcgcctc
    14641 ccccggcata gccggtttgc acgctataac tgaggttgtt gtcttccagc aacgtaccgt
    14701 atacaccagc cagattggtc atccgaccgt tgagatcgtg tgacatgctg tagctggcac
    14761 tggcatgtcg ccactgagat ttactgtcag aacgcagcca gtggctgaaa ggaatattga
    14821 cgttaagcgc taacatctga tcccgtcctt tttgccaggc gtttttcgtc aggctatagc
    14881 tgagggtcca gttgatatct tcgaacgcag tatttaatcc agcctggaat tgctcatcga
    14941 cattactcgt tccccaataa gtttgatggc taccactcaa atacagtgtt gatgtgcgcc
    15001 cgagttgctg agtaacggtg agctgtaatt tcccgcgttt gttataagcg aggttgtaat
    15061 agtcggtgaa tttcggctta acctgaataa ctccatcctg tgtttcgatg ttgtagccat
    15121 tcattcgact gtatgttgta tcagcgaaat taaaatatcc gctggtcgaa taacggtaac
    15181 ccactaactg aatattcgtg cctgattcat tgagcgattt gttatagaga aaacgcaccg
    15241 attgtccgtc atgctgactg tcatcgggaa gtgtggaatt agcctgcgtc atatccacag
    15301 acagagcgcc cagtgcctcc atgtttttcc cgataccgaa attaaaagca cgataacgat
    15361 ccgccagttg cgttccacca tatattgtcc agccagccgg aaggccgtgg agtaatgtac
    15421 tctggaaaaa gcggggtttt tcctgctgcg catttccact acggtattct cctgccgtaa
    15481 tggaataacg agtatgccct tcacgttgca aaagcgggac tgacgaatag ggtacggtaa
    15541 aaatctgcgt gctgccgtca gcctctttga tcgttacctg caagtcacca ctattacctg
    15601 cggcatagat atcattgatg gtaaaaggcc ccggtggcac cgtactatta taaatgccat
    15661 atccattttg tttaatagtg acctgtgcag taccatgagc aataccgtgg atcaccgggg
    15721 caaatcctct ttggctatcg ggtaacatat tgtcatctga ggccaattgt gcgccgcgaa
    15781 agttaatacc atcgaaaata tcgccctggg tataaccatc acccaacgtc agccgggaac
    15841 gtaaaggaat tatgtctcgc tcaatccagg taatgatatg ctgccattta tttttgctac
    15901 ctgatgaact gtcgctactg ttataactcc aggtggtatt gtcgcgtaaa cgccacgcac
    15961 caatatttaa cccactctgt aggtttaaat atgcataatg gctgttaccc ccaatccgat
    16021 tctgtacact
//
LOCUS       DADMEQ010000086        15980 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.087, whole genome shotgun sequence.
ACCESSION   DADMEQ010000086 DADMEQ010000000
VERSION     DADMEQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.087"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..644)
                     /locus_tag="JNZ05_002790"
     CDS             complement(<1..644)
                     /locus_tag="JNZ05_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565961.1"
                     /translation="MNASSINQTYLHDKNPGDVISLYGTLDNIPLVVKECTGKNHQIN
                     VKAKLPQERSEKVEDMCERMKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYS
                     AKDVGPDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVP
                     KNNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLA"
     gene            complement(625..963)
                     /locus_tag="JNZ05_002791"
     CDS             complement(625..963)
                     /locus_tag="JNZ05_002791"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565962.1"
                     /translation="MSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            complement(1553..2494)
                     /gene="fabY"
                     /locus_tag="JNZ05_002792"
                     /gene_synonym="yiiD"
     CDS             complement(1553..2494)
                     /gene="fabY"
                     /locus_tag="JNZ05_002792"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HAY8565963.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(2539..2976)
                     /gene="dtd"
                     /locus_tag="JNZ05_002793"
     CDS             complement(2539..2976)
                     /gene="dtd"
                     /locus_tag="JNZ05_002793"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAY8565964.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2973..3845)
                     /locus_tag="JNZ05_002794"
     CDS             complement(2973..3845)
                     /locus_tag="JNZ05_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HAY8565965.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(3839..4438)
                     /gene="yihX"
                     /locus_tag="JNZ05_002795"
     CDS             complement(3839..4438)
                     /gene="yihX"
                     /locus_tag="JNZ05_002795"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HAY8565966.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(4537..5322)
                     /locus_tag="JNZ05_002796"
     CDS             complement(4537..5322)
                     /locus_tag="JNZ05_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY8565967.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(5356..6252)
                     /gene="yihV"
                     /locus_tag="JNZ05_002797"
     CDS             complement(5356..6252)
                     /gene="yihV"
                     /locus_tag="JNZ05_002797"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HAY8565968.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            6420..7316
                     /gene="yihU"
                     /locus_tag="JNZ05_002798"
     CDS             6420..7316
                     /gene="yihU"
                     /locus_tag="JNZ05_002798"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HAY8565969.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPVQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            7344..8222
                     /gene="yihT"
                     /locus_tag="JNZ05_002799"
     CDS             7344..8222
                     /gene="yihT"
                     /locus_tag="JNZ05_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HAY8565970.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNGLCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAVIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            8239..9480
                     /gene="yihS"
                     /locus_tag="JNZ05_002800"
     CDS             8239..9480
                     /gene="yihS"
                     /locus_tag="JNZ05_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HAY8565971.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLFDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWDPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            9594..10520
                     /locus_tag="JNZ05_002801"
     CDS             9594..10520
                     /locus_tag="JNZ05_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HAY8565972.1"
                     /translation="MSLIKVPCMQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTF
                     QGCHLVIPHKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGL
                     LAWRDWQISELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTV
                     AAPYGVGIHPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKK
                     IAATKIDHTFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMS
                     CPPNAFNSGIDLLLLEPGKPHRLFFNIYGQRK"
     gene            10719..12755
                     /gene="yihQ"
                     /locus_tag="JNZ05_002802"
                     /pseudo
     CDS             10719..12755
                     /gene="yihQ"
                     /locus_tag="JNZ05_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
     gene            12801..14186
                     /locus_tag="JNZ05_002803"
     CDS             12801..14186
                     /locus_tag="JNZ05_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8565973.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQKLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTLYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            14229..15632
                     /locus_tag="JNZ05_002804"
     CDS             14229..15632
                     /locus_tag="JNZ05_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8565974.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIAKLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            15700..>15980
                     /locus_tag="JNZ05_002805"
     CDS             15700..>15980
                     /locus_tag="JNZ05_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565975.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGG"
BASE COUNT         3953 a         4070 c         3893 g         4064 t
ORIGIN      
        1 gctaaaggaa catattccac tgactgcggt aattccttac atgcctccct catcattgcg
       61 gtatgcttta caaacaacgg tttttcactg cccaaaaaaa ctttctgctt aattgcacta
      121 agaaaacgca aatcaaaaat aattgttttt ggtttattat tttttggcac atttcctatt
      181 atcccgctaa gaaccttcct tgctgcatgg agattttttt gcacagcaca taacacaatt
      241 gatgaagcct tctccactgc attattgatt ttaattttaa aatccgcatg atatacgttg
      301 acgggcaatg caagtatatc tggacctaca tcttttgctg agtatttatt ggtgatcgac
      361 ggacgtgaac ccacgagaaa acttagctgc aaattatgct ctatattatc agctgttttt
      421 gtggtgtggt taaacataga gatgccacct ttcattcttt cacacatatc ctcaactttt
      481 tcacttcgtt cttgtggcaa tttggcttta acattaattt gatgattttt acccgtgcat
      541 tccttaacta caaggggaat gttatctaaa gtgccataca ggctaataac gtcaccagga
      601 tttttatcat gtaagtaagt ttgattaata cttgaagcat tcacaaagag ggtcaatatt
      661 ataactgttt ataacttcac ctacatcaag tgtgtagtgc ttactctgga gactgccatc
      721 cttatttaat tgtatatcaa tcaatatatc cccggacttt gtcaccatat ctttgccata
      781 aaccatttgc atagccattt cgaggagatt tttatctaac tgattgcggg cgttgagtaa
      841 gcgattacca aataatttat atactgttcc cgtcattgcc agcgctgcgc tttctggtac
      901 tttgcgtttc cacagtgagg tttctctatt tttaaacagg tcatattcat tattactcga
      961 caaaccatgc ttcagttcgt taacacaatc gcacagttgc tcctcacctt ccattttaag
     1021 gcaaagtgaa aattgattta atggcgttta cttcggtgat ttattactca ttagttgttt
     1081 ttctaaatta acaacagaat aactttcttg tttaatgaga ttaatattgg caaccattat
     1141 agcctccttc ccacggttta agagcaaaca tacaacataa tgattcataa catacagagc
     1201 agtaaatgaa tgatactgac aatgtatgcc agcccattat ctttgcataa tgtttgtgag
     1261 ctatagattt taaaaatatt tatgaaaaaa gtatggcacc agagggggtt ttgactatca
     1321 aaaatcgctc aaacctgaac taagatgatg agttaagcat tatctgcaat aattgccact
     1381 cagctctaca aataatattt aaatgctcct gaacagtgat gtagcaagac acgtatggtg
     1441 ccaacgaaga ataaaaattt ataaagttct tattcaatat aaccggaata gtcagggact
     1501 gatgtaaatg accggaatga atgagagagg cagcaccgcc cctctccata agctactctt
     1561 cttcgttccc gccctcttca tacgggccaa atggcttcgc gggcagaacg atatacgtgc
     1621 cttcaaacac tgcacccggc gtctcgtcgc caaagatttc gacctgcatc tgcacccgtg
     1681 cttttcgtcc gcgcgccaga cggtcgagat cgccgcttaa ggcaccaagg tcggctaccg
     1741 catggggttt accgctaatc ggtttgctgt agcggatatg cgcatccgcc agaataatcg
     1801 ttccgccgag gtggcgttca cgcagcatca gccagataag cccccaaccg gtgagcgtcg
     1861 ccagcgagaa taaactcccg gcaaacagcg tatggtgcgg attctgattg ccggtttccg
     1921 gcatggtggt gataaatttt tgcccggtat attgttgaat acgcacgccc attttttcac
     1981 taagcgggat atgttcgtac cacgcctgtt gcagctgcgc gcaccagtcg ccgcgatgca
     2041 aaatatcatc cagagtggcg acgggcttaa tcatcaaaaa atggcgaatc ggcgtggtgg
     2101 ttggcgtggt gatttctccc tgattaacaa accccagctt ggcgaaaaac tccaccgcgt
     2161 cttcacgggc gctacaggtc acgcgcttaa cgccttcctg acgcgccacc gactccaggg
     2221 tcatcgccat cagcgttcct aaccctttgt cctgcacgtc cgggtgaacg gccataaagc
     2281 gaatggacgc ttcattgtcg gcattaatat acaatcggcc taccgccacc agattaccct
     2341 gctcgtcgac gaccatctgg tgatgcgcca tcgcatccca cgcgtcgcgt tccgaacctt
     2401 ttggttgatg caggggctta cgcaacattt cccagcgaaa ctgatagtaa cgctctaact
     2461 cttcttctgt ttgtggaacc tgaaggtgat acatagcgat actctctctt gtttcccgtg
     2521 acaaccctgg aagctggctc atacctgcaa ccagaatgtc acggggccat cattgaccag
     2581 cgatacctgc atatcggcag cgaagcgtcc tgtttgcgtg ttcatctctt gctggcggca
     2641 gcgttcgacg aaatagtcat ataacgcctc tgcgcgatcc ggtgatgcac ctttggagaa
     2701 acttgggcgc atcccccgtt cggtatctgc ggcgagggta aactgggaaa ccaccagtac
     2761 actgccgccc gcctgttgca cgttgagatt catcttgcct tcggcatcgc taaagatgcg
     2821 gtagccgagc acacgctcgc acagacggtt tgctttctgt tcgtcgtcat ccttttcgac
     2881 acccaataac accaaaagtc ccgcgccaat ttcgcccgtc acttctccct ccacggtgac
     2941 gctggcacgg gttacgcgtt gaattaatgc aatcatggtt cgtcgtcttc ttcttgttca
     3001 gctgcttgtt ttagtttgcg gtattccccg agagtgacag taatttccgc gccaagcaag
     3061 acgatacacc atgtccagta gacccaaaca aagagaatgg ggatcaccgc cagcacaccg
     3121 taaatgagct gatatgacgg gaacatggtg atataaagcg cgaaaccttt ctttcctgct
     3181 tcgaacagga gtgcggcgac aaacgcgccg acaatcgcgt cgcggttagg tacgcggatg
     3241 gtaggaacaa tgctgtacag caaccagaag gagatccacg acaacagcaa cggaaaaata
     3301 cgcagcacgt tgtcgatgac agtattgaga tcgctcgccc agcgcaggga gagcaaatag
     3361 gaactgatcg ccagactggc ccctgccagc agcggcccca gcgttaaaat catccagtac
     3421 acggcgaacg agtaaatttt tggtcgcgct cgtttactgc gccagatggt attcaacgcg
     3481 ctatcgatgg agtacatcaa caataacgcg gtgacgatca gcccgcacgc cccaacggcg
     3541 gtcatcttgt tggaattagc aacaaattgt tcgatatacc gctgaataac atcgccagta
     3601 gcaggcagaa agttggcaaa aataaagtga cgtaactgaa tgctgacgtc ggaaaacatg
     3661 ggaaaagcgg cgaaaagcgc aaaaacaacg gcaaccagcg gcactaatga gagcaacgac
     3721 acataggcaa ggttacctgc cagggttgtc atgttgtcct catcaatacg ttgccagagt
     3781 agttttagcc aggcccatag tggacgggta cggtgcctgg ctttgtcctg aatggttttt
     3841 agcataacac cttcgcgaaa tagtccggga tggtggtttt atctttcacc agaatactgg
     3901 taatgcctag ctgattagct ccttctatat tatcggcgtt atcgtcgaaa aagaccgtat
     3961 cgctgggtga aaaaccttcc gcctgcaaaa catgctggta aattcgtgct tcaggtttgc
     4021 gcatccccag atcttgcgac agatagatat ggtcagcagc atcacgaatt tccgggtatt
     4081 cttccggcca gaaggtggta tgcaggcggt tggtattgga aagcaccacc acgcgatgcc
     4141 cctgctcacg cagtttatgc atgatggcga tcacttccgg gcgcagcgca acaaacaccg
     4201 cctgccagcc gtgagagaac tgctcgtagc ttagcggtag agccatctca tgacacagcg
     4261 cctctgcgaa cgcttcgtcg ctaatttccc cacgctcatg ctgatgaaac gcctccccca
     4321 tatgaaaact cttcttaagc gatgccagcg gaatacgcgt taaatcgctc caggctccca
     4381 gcacacggtt aaagtcgata tcgacaatca cattacctaa atcaaagata tagagcattt
     4441 ttgcctcctt tcgcgccaca agaaattaac tgtagcggga aaggatgggt ttggctatgt
     4501 ggcaaggtat gaagccaggt taacggtcac aaaaatctac gcgtcttcct ggggaagaat
     4561 gatttcaatt tggctgtctt gtaagcgtga ttttagcgca gcgggcggca gctcgtcggt
     4621 aaagagcgca gtgacctgtg ccacgttacc aatttcaacc gccgccgaag catgatactt
     4681 agtgtgatcg gcgaccagca gaatatttct cgcgtgcgcc atcatcgttt tcaccacgtt
     4741 agcttcgttt acatcaaact ccatcaacgc gccatcgctc tcaatcgccc caacgcttgt
     4801 taccagataa tcagcacgaa aatcagccac aaaggacgcc gctgaagggc caatgatccc
     4861 gctattatga gagcgcaacg taccgccggg caccatcact tcaaagcgcg ggttgtggta
     4921 aagaatatgc gccacacgca ggctgttggt gattatccgc aaatgattat ggttaagtaa
     4981 cgcccgggca acatgctcaa cagtcgtacc aatggtgata aatattgttg aaccatcagg
     5041 aatatagtct gccaccgctt cggcaatcgc tttttttttc tcggtttgcg aaacctcacg
     5101 ctgctcgaac gccgtattaa cgacgctgga agcccgaccc gcgccaccgt gatggcgcgt
     5161 aatcaggcct tgctcgctta atttacgaat atcccggcgg accgtctgcg tggagacatc
     5221 cagcagattt gccagctcat caatattcat atacccacgc tcggcgatca gcatgaggag
     5281 ttggtcgtgc cgcgggttac cggttagttc ggtaaggctc atgaaaattc ctcgaaaaac
     5341 catcaccctg gcattttata caaaaagtga caaaaaagat cgggtttgat cacagtcagg
     5401 gatcccggct cgtccaccgg gacgtgtgca ttttaacgcc gctacaccgc tggcgaagcg
     5461 gactgactcc gctaaatccc cacttgttgc cagcgccacc gccaaagcgc cgtgaaaaac
     5521 atcacccgca cctgtggtat ctaccacatc aactttgaag gccggttgat gctgacgccc
     5581 accattttcc agccagtcgc agcctgcgct accctgggtc acatagacat gaccatttgt
     5641 gagcgtttgt gcctgtttta gcgcactggc catctctttc acgcccgtta agcgcgccag
     5701 acccggttct gaaaaggccg cgtgatcgct taatgccacc agctcactga tatcctgcgg
     5761 cgtaatgtcc ccgtccagaa cggtcatcac acccgcctga cgggccaggg tgaaggcttt
     5821 tttagcgccg tcgtgccagc gtacatctgc cagcacaaca tcccactgag agaaatcaat
     5881 ttcctccaac cactctgcgt caggcagcag atccgggctg gggtagttaa tgattatccg
     5941 ctcgcctttg gtatccacca tgatggcgga ttgcgaagat ttcgcctggt tataccgttt
     6001 ggtgtaacgg gtgttaacgc cccaggattc cagctctgct agcaggctgt tgcccgcgtc
     6061 gtcatcacct acgcgaccaa taaaatcgac ctgcgccccc agccttgccg ccgcaaccgc
     6121 tgccgtcgcc gctggcccgc caccaacttc cgtataattt ctcgccacgt atttaccgct
     6181 ctccgtcggt aacccttcca cgtaatagat gcgatccatc acggttatac ctacacaagc
     6241 aacacgaatc atggtcattc cttaaacatt ttgggatgcg ttcattttca tttcaccaat
     6301 tgtttaaaaa gtgacggctg tcaatttttt gacgataaat tacaaattcg atcaaaaaca
     6361 gacaagcaaa gcatgtcata aatgacaaaa agtgacatca ctgtattcag gagagggtta
     6421 tggcagcaat cgcgtttatc ggtttaggac aaatgggttc gccaatggcg agcaatttat
     6481 tgcagcaagg gcatcaactt cgcgtcttcg atgtgaacgc cgaagctgtg cggcatctgg
     6541 tagacaaagg tgcgactccc gccgccaacc cggtgcaggc cgctaaagat gccgaattta
     6601 tcattaccat gttgccgaat ggcgatctgg tgcgcaacgt gttgttcggt gaaaacggcg
     6661 tttgcgaagg cttatctacc gatgcgctgg tcattgatat gtccaccatc catccgctgc
     6721 aaaccgataa attgattgcc gatatgcaag ccaaaggctt cagcatgatg gatgttccgg
     6781 taggccgtac ttctgcaaat gccattaccg gtactctgtt actgctggct ggcggcaccg
     6841 ctgaacaagt tgaacgtgcc acaccgatcc tgatggcgat gggcagtgag ttgatcaacg
     6901 ctggcggtcc gggcatgggg atccgcgtta agctcatcaa caactacatg agcatcgcgc
     6961 tcaatgcgct ttcggcagaa gccgccgttt tgtgcgaagc cctgaatctt cccttcgatg
     7021 ttgccgtcaa agtgatgagc ggtaccgccg ccggtaaagg tcacttcacc acttcctggc
     7081 caaacaaagt cctcagcgga gatctttctc ccgccttcat gatcgatctt gcccataagg
     7141 atcttggcat cgcccttgat gtcgccaacc agctgcatgt gccaatgccg ctgggggccg
     7201 cctcacggga ggtttatagc caggcgcgcg cagcgggtcg cggtcgccag gactggtccg
     7261 ccattctgga acaggtccgt gtcagtgccg ggatgactgc caaagtaaaa atgtaacgac
     7321 tggataaagg aataaatgaa tgaatgaata agtacaccat caacgacatt acgcgcgcat
     7381 cgggcggttt tgccatgctg gcggtcgatc agcgcgaagc catgcgcatg atgtttgccg
     7441 cggctggggc acccgctccg gtagccgata gcgttttaac tgatttcaaa gttaacgctg
     7501 caaaggccct ctcgccttat gcctcggcga ttctggtaga tcaacaattc tgctaccgcc
     7561 aggtggttga gcaaaacgcg attgccaaaa gttgcgccat gattgtcgcc gccgatgagt
     7621 tcattcctgg caacggtatt ccggtcgata gcgtggttat tgaccgcaaa atcaatccgc
     7681 tacagatcaa acaagacggt ggcaaagcct taaaactgct ggtgctgtgg cgtagtgatg
     7741 aagatgcgca gcaacgtctg gatatggtga aagagttcaa cggactgtgc cactcacacg
     7801 gtctggtaag catcattgag ccagtcgtcc gcccaccgcg tcgtggcgat aaattcgatc
     7861 gcgaacaagc ggtcatcgat gccgccaaag agctgggcga cagtggcgct gacctctaca
     7921 aagttgaaat gccgctttat ggcaaaggtc cgcaacaaga gcttctctgt gcttcacaac
     7981 gtctgaatga ccatatcaat atgccatggg tgatcctctc ttccagtgtc gacgaaaaac
     8041 tgttcccgcg tgccgtacgc gtggcaatga cggcgggcgc atcgggattc ctggcaggcc
     8101 gtgcagtctg ggcatcggtc gtcggtttac cagacaacga gctgatgctc cgtgacgttt
     8161 gcgcaccgaa attacaacaa cttggcgata tcgtcgacga aatgatggct aaacgccgct
     8221 aagatgagga taaaaggaat gaaatggttt aacaccctaa gccacaaccg ttggctggaa
     8281 caggaaaccg accgcatctt tgattttggt aaaaattccg tagtgccgac tggttttggc
     8341 tggttaggca ataaagggca aatcaaagaa gagatgggca cccatctgtg gatcacagct
     8401 cgtatgttgc acgtttattc cgttgctgcg gcgatgggtc gacctggcgc ttacgcgttg
     8461 gttgatcacg gtattaaagc catgaacggc gcactgcgtg ataaaaaata tggcggctgg
     8521 tatgcctgcg tgaatgacga gggcgtggtg gatgcctcca aacagggcta tcaacacttc
     8581 tttgctctgc tgggtgccgc cagcgccgtc acaacgggtc acccggaagc gcgcaagctg
     8641 ttcgattaca ccattgagat tatcgagaaa tatttctgga gcgaagaaga gcagatgtgc
     8701 ctggaatcct gggacgaagc cttcagcaaa accgaagagt accgcggcgg caatgccaat
     8761 atgcacgcgg tggaagcttt cttgattgtt tatgacgtca ctcacgacaa aaaatggctg
     8821 gatcgcgcga ttcgcgtggc ttccgtgatt atccacgacg tcgccagaaa taatcattat
     8881 cgcgttaacg aacacttcga tacccagtgg gatccgctgc cggattacaa caaagataac
     8941 ccggcgcatc gcttccgcgc gttcggaggt acaccaggcc actggatcga atggggccgt
     9001 ttaatgctgc acatccatgc tgccctggaa gcccgttgcg aacaaccacc agcatggctg
     9061 ctagaagatg ccaaaggtct gtttaacgcc accgtgcgcg atgcctgggc acccgatggc
     9121 gcggacggga ttgtttatac cgttgactgg gaaggaaaac cggtggtccg cgaacgtgta
     9181 cgttggccta tcgtcgaagc aatgggtacg gcctacgcgc tctacaccgt cactggcgat
     9241 cgccagtatg aaacctggta tcaaacatgg tgggagtact gcattaagta cctgatggac
     9301 tatgaaaatg gttcctggtg gcaggagctg gatgcggaca ataaggtcat aaccaaagtc
     9361 tgggacggca aacaggatat ttatcacctg ctgcattgcc tggtgatccc gcgtatcccg
     9421 ttagcgcccg gcatggctcc agcggttgca gcgggtctgc tggatattaa tgcgaaataa
     9481 ctgatctgaa aatagggtgc gggttttccc ctcaccctaa ccctctcccc aaaggggcga
     9541 ggggaccgtc cggtgcagtt tttgggtttc acatcagcct cacgggaggc tgtatgtcgt
     9601 taattaaggt gccttgtatg caaatcacca atatgcattg cagtggacag accgtttcac
     9661 ttgccgcggg ggattatcac gcgaccattg tgaccgtcgg tgctggtctg gctgaattaa
     9721 cctttcaggg ttgtcattta gtcatcccgc acaaacccga agagatgccg ctggcgcatc
     9781 tgggtaaagt gcttattccg tggccgaacc ggatcgccaa cggctgttac cgttatcagg
     9841 ggcaggaata tcagttaccc attaatgaac atagttcaaa agccgctatt cacgggctgc
     9901 ttgcctggcg agactggcaa attagtgaat taaccaccac cagcgttacc ctgacagctt
     9961 ttctgccgcc cagttacggc tatccgttta tgctggcttc acaagttgtt tattcgctca
    10021 acgcacacac gggtctttcc gttgagatcg caagccagaa tatcggcact gtcgcagcgc
    10081 cttatggcgt gggtattcat ccttatttaa cctgcaactt aacatctgtt gatgaatatc
    10141 tgttccagct tccggcaaac caggtttatg ccgtcgatga acacgtaaat ccgacaacgc
    10201 ttcaccacgt tgatgagctg gatttaaatt tcacgcaggc gaaaaaaata gccgcgacaa
    10261 aaatcgacca cacctttaaa accgctaatg atttgtggga aatgaccatt acccatccac
    10321 agcaggcgct ttcggtaagc ctttgttccg atcaaccctg ggtgcaggtt tatagcggtg
    10381 aaaaattaca gcgtcaggga ctggcagttg aacctatgag ttgcccgcct aacgccttta
    10441 attcaggtat cgacttatta ttactggaac caggaaaacc gcaccgcctg ttttttaata
    10501 tttacggaca gagaaaataa cttgtattga ttagcagtca aaatcattga agaacatttc
    10561 actcgctaaa ggcaacatta tgtatcttca atttattaag ttaaaaacag ctcagcttct
    10621 gtaaagtttt tatatatcag ttttatgaga atcattttac ataagcctgt aataacagga
    10681 aggcaacaat ctcgttctca actctatgga gattagttat ggatacgcca cgtccacagt
    10741 tattagattt tcaatttcat cagaataacg acagttttac cctacatttt caacaacgtc
    10801 ttattttaac ccatagcaaa gataatcctt gtttatggat tggctcaggt atagcggata
    10861 tcgatatgtt ccgcggtaat ttcagcatta aagataaact acaggagaaa attgcgctta
    10921 ccgacgccat cgtcagccag tcaccggatg gttggttaat tcatttcagc cgtggttctg
    10981 acattagcgc cacgctgaat atctctgccg acgatcaggg ccgtttattg ctggaactac
    11041 aaaacgacaa ccttaaccac aaccgtatct ggctgcgcct tgccgctcaa ccagaggacc
    11101 atatctacgg ctgcggcgaa cagttttcct acttcgatct gcgcggcaaa ccgttcccgc
    11161 tatggaccag tgaacaaggc gttggtcgca acaaacaaac ctatgtcacc tggcaggccg
    11221 actgcaaaga aaatgcgggc ggcgactatt actggacttt cttcccacag cctacgtttg
    11281 tcagcacgca gaagtattac tgccatgttg ataacagttg ctacatgaac ttcgacttta
    11341 gcgccccgga ataccatgaa ctggcgctgt gggaagacaa agctacgcta cgttttgaat
    11401 gtgctgacac atacatctcc ctgctggaaa aattaactgc cctgctggga cgccagccag
    11461 aactgcccga ctggatttat gacggggtaa cgctcggcat tcagggcggg acggaagtgt
    11521 gccagaagaa actggacacc atgcgtaacg cgggcgtgaa ggttaacggc atctgggcgc
    11581 aggactggtc tgggatccgt ataacctctt ttggcaagcg cgtgatgtgg aactggaagt
    11641 ggaacagcga aaactacccg caactggatt cacgcattaa gcagtggaat caggagggcg
    11701 tgcagttcct ggcctatatc aacccgtatg ttgccagcga taaagatctc tgcgaagaag
    11761 cggcacaaca cggctatcta gcaaaagatg cctctggcgg tgactatctg gtggagtttg
    11821 gcgagtttta cggcggcgtt gtcgatctca ctaatccaga agcctacgcc tggttcaagg
    11881 aagtgatcaa aaagaacatg attgaactcg gctgcggcgg ctggatggct gacttcggcg
    11941 agtatctgcc caccgacacg tacctgcata acggcgtcag tgccgaaatt atgcataacg
    12001 cctagcctgc gctgtgggcg aagtgtaact acgaagccct tgaagaaaca ggcaagctcg
    12061 gcgagatcct cttctttatg cgcgccggtt ctaccggtag ccagaaatac tccaccatga
    12121 tgtgggcggg cgaccagaac gtggactgga gtctcgacga tggcctggcg tccgtagtcc
    12181 cggcggcgct gtcgctggca atgaccggac atggtctgca tcacagcgac attggcggtt
    12241 acaccaccct gtttgagatg aagcgcagca aagagctgct gctgcgctgg tgcgatttca
    12301 gcgccttcac gccgatgatg cgcacccacg aaggtaaccg tcctggcgac aactggcagt
    12361 ttgacggcga tgcggaaact atcgcccatt ttgcccgtat gactaccgtt ttcaccaccc
    12421 tgaaacctta tctgaaagag gccgtcgcgc tgaatgcgaa gtccggtctg ccggtcatgc
    12481 gcccgctgtt cctgcattac gaagacgatg cacataccta ttccctgaaa tatcagtacc
    12541 tgttaggccg cgacattctg gtcgctccgg tacacgaaga aggtcgcagc gactggacgc
    12601 tctatctgcc ggaggataac tgggtacatg cttggacggg tgaagcgttc cggggcgggg
    12661 aagttaccgt taatgcgccc atcggcaagc cgccggtctt ttatcgcgcc gatagcgaat
    12721 gggcggcact gttcgcgtcg ttaaacagca tctaatcaca tccgcccgtg gggaaactca
    12781 tgggcaaagg gagaacatcg atgagtcaca tcacaacgga agatccagca actttacgcc
    12841 tgccctttaa agagaaactc tcttacggta ttggcgacct ggcctctaac atcctgctgg
    12901 atatcggtac gctttatctt ttgaagtttt ataccgacgt tctggggctg ccaggcacct
    12961 atggcggcat tatctttttg atttcaaaat tctttactgc gtttaccgat atgggtaccg
    13021 gcattatgtt ggattcccga cgcaagatcg gtccaaaagg taagttccgt ccttttattc
    13081 tgtatgcgtc attcccggtc accttactgg cgatcgccaa ctttgtcggc acaccgtttg
    13141 atgtcaccgg taaaacggtg atggccacta ttctgtttat gctttacgga ctgtttttca
    13201 gcatgatgaa ctgctcctac ggcgcaatgg tcccggcaat caccaaaaac cccaacgaac
    13261 gcgcctcact ggcggcatgg cgtcagggcg gcgctacgct gggcctgctg ctgtgcacgg
    13321 tgggattcgt gccagttatg aatcttatcg aaggtaatca gaaacttggc tatatcttcg
    13381 ccgccacgct gttttcactg ttcggcctgc tgtttatgtg gatctgctac tcaggcgtga
    13441 aagagcgtta tgtcgaaacc caaccaacca atccggcgca aaagcctggc ctgttgcaat
    13501 ctttccgcgc aattgccggt aaccgcccac tgttcattct gtgtattgcc aacctctgca
    13561 ccttaggggc gtttaacgtc aagctcgcca ttcaggtcta ttacacccag tacgtactta
    13621 acgatcccat cctgttgtcg tatatgggat ttttcagcat gggctgtatt ttcatcggcg
    13681 tgttcctgat gcctggcgca gtcagacgtt ttggtaagaa gaaggtctat atcggcggcc
    13741 tgctgatttg ggtgctgggc gatctgctca actatttctt cggcggcggc tcggtcagct
    13801 ttgtggcgtt ctcctgcctg gcgttcttcg gctcagcgtt tgttaacagc ctgaactggg
    13861 cgctggtttc cgacaccgtc gagtacggcg agtggcgcac cggcgtgcgt tcggaaggca
    13921 cgctctacac cggcttcacc ttctttcgca aagtgtctca ggcgctggct ggtttcttcc
    13981 ccggctggat gctgacgcaa attggctatg tgccgaacgt cgcacaggct gaccacacca
    14041 ttgaagggtt acgccagttg atcttcatct acccaagcgc actggcggta gtcaccattg
    14101 tggcgatggg ttgcttctac agcctgaacg agaagatgta tgtccgcatt gtggaagaga
    14161 tagaagcccg taaacgcacg gcgtaataat aataaacgac gccctgcggg gcgttataag
    14221 gagtgattat gtctgaccat aatccactga cattaaaact gaatctgcgg gaaaaaatcg
    14281 cctatggtat gggcgacgtc ggttcgaatt taatgctctg catcggtact ctgtatctcc
    14341 tcaaacttta taccgatgaa ttagggatgc ctgcttacta tggcgggatt atctttctgg
    14401 tcgcgaagtt ttttaccgcg tttaccgata tgctcaccgg atttttactc gactcgcgga
    14461 aaaatattgg gcctaaaggc aaatttcgtc cctttatttt atatgcagca gttccggcgg
    14521 cgttaattgc aacgctccag tttatcgcca ccaccttttg tttgccggtt aaaacgacga
    14581 ttgccaccgc gctgtttatg atgtttggac tttcatacag tctgatgaac tgctcgtatg
    14641 gtgcgatgat cccggcaatt accaaaaacc cgaatgagcg cgcgcagctc gcggcttacc
    14701 gccagggtgg tgcaaccata gggctattga tttgtaccgt ggcgtttatt ccgttgcagt
    14761 cgcttttttc tgactcaacc gtcggttatg cctgtgcggc acttatgttc tccattggcg
    14821 gctttatttt tatgatgctg tgctacagag gcgtcaaaga gcattatgtg gacacagcgc
    14881 caaccggaca taaagccagt attctcaaat ctttttgcgc gatatttcgt aatccaccat
    14941 tgctggtttt atgcattgct aaactgtgta ctctggcggc atttaatatc aaactggcga
    15001 ttcaggtcta ttacacccag tatgtgctga atgatattaa tttattgtcg tggatgggat
    15061 tcttcagtat gggatgcatc ctcgtcggcg tattactggt gccagtaact gtaaaatgtt
    15121 ttggtaaaaa acaggtttat ttagctggca tggtgctatg ggcggtgggt gatatactga
    15181 attatttctg gggaagtaac tctttcactt tcgtcatgtt ctcttgtgtc gccttttttg
    15241 gcacggcctt tgtaaacagc ctgaactggg cactggtacc agataccgta gattacggtg
    15301 aatggaaaac cggtattcgt gccgaaggtt ctgtttatac cggttatacc ttctttcgta
    15361 aaatttctgc cgcacttgct ggcttcttgc caggcattat gctgacgcaa attggttatg
    15421 ttcccaacat cgcgcaaagc gatgcgacat tacagggttt gcgtcagctc atttttatct
    15481 ggccttgtgc actggcaatt attgctgcat tgacgatggg attcttttac acactcaatg
    15541 aaaaacggtt tgcattgatt attgaggaaa tcaaccaacg caaaaataaa gagatggcga
    15601 cagaaaaaaa aacggcttcc gtaacgttat aaatgtcaat agccgctatt tccatcctgg
    15661 tgggtggcgg cctccctacg tttaaaaaat ggacttatta tgaaaaagat taatgcaata
    15721 attttattat cttctctgac ctctgcctcg gtatttgctg gcgcatacgt cgaaaacagg
    15781 gaggcatata atcttgcctc cgatcagggc gaggttatgc tacgtgtggg ttataacttc
    15841 gatatgggcg cgggtattat gttaaccaat acctacacct ttcagcgaga agatgaacta
    15901 aaacatggct ataatgaaat tgaaggttgg tatcctttat tcaaaccaac cgataaatta
    15961 accatccagc ccggtggctt
//
LOCUS       DADMEQ010000087        15952 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.088, whole genome shotgun sequence.
ACCESSION   DADMEQ010000087 DADMEQ010000000
VERSION     DADMEQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.088"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="JNZ05_002806"
     CDS             complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="JNZ05_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HAY8565976.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(998..2020)
                     /locus_tag="JNZ05_002807"
     CDS             complement(998..2020)
                     /locus_tag="JNZ05_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8565977.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADICGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2034..3536)
                     /locus_tag="JNZ05_002808"
     CDS             complement(2034..3536)
                     /locus_tag="JNZ05_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY8565978.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLGGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3676..4633)
                     /gene="ytfQ"
                     /locus_tag="JNZ05_002809"
                     /pseudo
     CDS             complement(3676..4633)
                     /gene="ytfQ"
                     /locus_tag="JNZ05_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134480.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4943..5473
                     /gene="ppa"
                     /locus_tag="JNZ05_002810"
     CDS             4943..5473
                     /gene="ppa"
                     /locus_tag="JNZ05_002810"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HAY8565979.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5853..6194)
                     /locus_tag="JNZ05_002811"
     CDS             complement(5853..6194)
                     /locus_tag="JNZ05_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY8565980.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6197..9976)
                     /gene="tamB"
                     /locus_tag="JNZ05_002812"
     CDS             complement(6197..9976)
                     /gene="tamB"
                     /locus_tag="JNZ05_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HAY8565981.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGIYTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9973..11706)
                     /gene="tamA"
                     /locus_tag="JNZ05_002813"
     CDS             complement(9973..11706)
                     /gene="tamA"
                     /locus_tag="JNZ05_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HAY8565982.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGGGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11912..12550
                     /gene="msrA"
                     /locus_tag="JNZ05_002814"
     CDS             11912..12550
                     /gene="msrA"
                     /locus_tag="JNZ05_002814"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAY8565983.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGLYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12873..14216
                     /locus_tag="JNZ05_002815"
     CDS             12873..14216
                     /locus_tag="JNZ05_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY8565984.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14278..14484)
                     /locus_tag="JNZ05_002816"
     CDS             complement(14278..14484)
                     /locus_tag="JNZ05_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HAY8565985.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14809..15363
                     /locus_tag="JNZ05_002817"
     CDS             14809..15363
                     /locus_tag="JNZ05_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY8565986.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15426..>15952)
                     /locus_tag="JNZ05_002818"
     CDS             complement(15426..>15952)
                     /locus_tag="JNZ05_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8565987.1"
                     /translation="ACDILQKKQSDIPKRIKGYIRQAFGDVSFGYALLEDVVSKLKRG
                     KFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLGFALKIYGGDERFRNGSSVVLED
                     EDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSNITKEKICEKYKSIIEAFKKEQ
                     LALPVLVRCKNSET"
BASE COUNT         3797 a         4372 c         3922 g         3861 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccacc aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacgat gcgcaaggaa
      481 aataccgata accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc
      541 ggggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga
      601 ctcttccgaa acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat
      661 aaatgccggg atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc
      721 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt
      781 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
      841 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
      901 attgcagatc acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
      961 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     1021 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     1081 ccgctttcac cacctggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca
     1141 tcccctgaat aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca
     1201 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt
     1261 tgttggcatc ggcaccgcaa atatccgccg ccacgataat gcccgcaatc gccgcacata
     1321 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
     1381 ccgcccgaat gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca
     1441 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     1501 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     1561 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     1621 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     1681 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     1741 ctccggcgat agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca
     1801 gcgtcatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     1861 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     1921 cgaccagcag caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac
     1981 gcctttttgg cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc
     2041 gcaatggcgt tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt
     2101 ttgcgatcgc gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag
     2161 gagatcacca gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     2221 gcgtgggcac caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg
     2281 gtcagtagcc aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc
     2341 ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct
     2401 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcaaggatg
     2461 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
     2521 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     2581 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     2641 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     2701 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
     2761 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     2821 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     2881 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     2941 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccacttcc
     3001 tgggtgtcga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     3061 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     3121 acgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     3181 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac
     3241 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     3301 gcggtatttt tcggtgagat agtctggcct cccagccaga tggtgccgcg atcggcgtgg
     3361 tatacaccag ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc
     3421 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     3481 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     3541 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     3601 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     3661 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     3721 atcaggcagg taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
     3781 tttgtatttc tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact
     3841 ggcgttcgct tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc
     3901 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     3961 caccatgtcg tcgttatggg cgtaaaccat gcagatgttt ttgccgttgt tttccgcttt
     4021 gataaagctc tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     4081 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct taacggtcaa
     4141 tggcgacgct ggccccaacg gtgccctgca gctccaccac gttgcatggt ttgccattca
     4201 cttcttttac cagccagtca ccgatcaact taccttcgag gatgttgtcg gcagtaacgg
     4261 tggtcatata gagagatttg tctttcacat cgatggaacg gtcgagcaag aagaccggga
     4321 tttcggcatc tttcgcctct tttaataccg gttcccaacc cgtcgcaacc actggagcga
     4381 taaagatcgc atccacccct tgtgcaacga aggaacgtac cgctttaatc tggttttcct
     4441 gcttttgctg accatcggca attttcaacg taattccgcg tttttcggcc tcacttttcg
     4501 ccacattggt ttctgcggcg cgccagccgg attccgatct gacctgcgaa aatcctacgg
     4561 ttaatggagc ggccaacgcc atagacgaca tggctgccga gactgcagag actacaagta
     4621 agcgtttcca catagggttg tcctcgtcgg ggtaattatt attggtgaaa agatgttcgc
     4681 gaaaaaacta tagacaattc gttatgtaac ggattgcgtt acatcacact tcaaaaaagt
     4741 aaataaaacg ttaatcacaa gtttgtaatc gctttcatct cactatgaaa aatgcggcaa
     4801 cggttatgga ttttcctgct ctgtataccg tcttaaaact ggcgaaaaag gaaaatgaag
     4861 acgaaaacaa gcaaagacat tcggcgcgag ttggctataa tacttggcac ttgtttgcca
     4921 catattttta aaggaaacag acatgagctt actcaacgtc cctgcgggta aagatctgcc
     4981 ggaagacatc tacgttgtta ttgagatccc ggctaacgca gatccgatca aatacgaaat
     5041 cgacaaagag agcggcgcac tgttcgttga ccgcttcatg tccaccgcga tgttctatcc
     5101 gtgcaactac ggttacatca accacaccct gtctctggac ggtgacccgg ttgacgtact
     5161 ggtcccgact ccgtacccgc tgcagcctgg ttctgtgatc cgttgccgtc cggttggcgt
     5221 tctgaaaatg accgacgaag ccggtgaaga tgcgaaactg gttgcggttc cgcacagcaa
     5281 gctgagcaaa gaatacgatc acattaaaga cgtaaacgat ctgcctgaac tgctgaaagc
     5341 gcaaatcgct cacttcttcg agcactacaa agacctcgaa aaaggcaagt gggtgaaagt
     5401 tgaaggttgg gaaaacgcag aagccgctaa agctgaaatc gttgcttcct tcgagcgcgc
     5461 aaagaataaa taagttcttc tagcgcaata accctgaacg ccgggcttcg gttagtaagg
     5521 gtttttttat gcccgcgata aataaactct ctattccacc atcattattc tcagcggttg
     5581 caaggcttga acggtaagaa caagcaaacc cgaccaccat tttgctgttc atagccactt
     5641 gctggaagtt agcagacctc actcatactc accgttcaaa gcgtgccccc gttttacccc
     5701 tgcacatttg tgaacgccag cagagttttt tatgcgattg cagatatgga aagcggctcg
     5761 gagagaaaag caaaagatgg aaaagttaca aaaaaaaatc gcgagataca tcgcataaaa
     5821 caacgccacc cgaaggaggc gtgcttttat tattacttat ccctgtctaa ccagtcgccg
     5881 ctttcaatta gctttaatcc atcgacgggt cgttgataaa cgtacatcca tgcactccca
     5941 tacggcgtct gaatcaactg gcgcgcgtat tcaccgcccc tggtgcgcaa ggcatcaagt
     6001 tcggccagcg tggcgttgtc aatacgataa acttcaccgt gtaccgttcc gttccccgga
     6061 actgcgcctg gatagtggcc caggctatac aactggtagt tatcgatact gaaatcgccc
     6121 agtaactggg cattggtcat ccagtgactg ttgccttgtt tgtggcgtaa actgccgtag
     6181 acaaatattc gcattgctaa aactcgaact gatagagcaa atccagtgcc tggtctacac
     6241 cagacacggc ttccagatat agcttaggca tcaggcgata acgtaacgtg agtgttgcta
     6301 tagagtcaaa tatacccacg ccgtatttca cttgcagacc tggcaataca tagccgctga
     6361 ccactacctg ggaggagtcg cctactccca gggtgtcgag cgctaaattg cttacgccaa
     6421 acgtctcgcc gattttaccc acaatctggc cactttgcgc aacccccaga ccaatcagca
     6481 tcgaggtcat tgccgcactg tcgctctgat cgctctccag cccttgtcca cgtagcaaat
     6541 aagacaatgc agcttgttgc gacatcgccg ggtcagagaa gatctccgct ttcggttcgt
     6601 ccgccagacc agtgacgcga actccggcga ttacatcgtc ttctgtagca tccgggttac
     6661 gaatagcttc aatattaaga tacggttgat ccggcggacc agagaacagt aactcaccct
     6721 tacgcacaat cagatcctga ccataggcat ggaagcgccc ttcagggatg ttgatctgcc
     6781 cgttcaggcc cagcccttgt ttgtcctgta cgacgttaag atcgcccgtc agccgcgctt
     6841 tcaggccaaa ggcgtcaatg cgcacattgt tgccaacgtg gacaatcagg ttactgttaa
     6901 tcggaatcga cgccgttttc ggctcttccg gttgcaggtt atcgttaagc atcaccacat
     6961 cgctggagac gcctactgcg ctttccggca gatcgtgcac cacgatgcgc gcccacggga
     7021 catccacgcg accatcgagg gtaaacaggt ttggtgtagc ctcgaataca acatctggcg
     7081 atacatccat tcgtaccatc ggcggcacgg tgatccgcac tttactgcct ttcgccgtta
     7141 ctcgtgcccg ccagttttca atttggctcc agtcggcatc accgttcagg tagatttcac
     7201 cctgctgggt ccgtactgta ccggcaagcg tcgagcgcat accgttaaag ttgaccgcaa
     7261 gctggctcgg ctgcatatca aacggcataa agttgccgtc gatatccaca cccgtaacct
     7321 gaagctgacc aaacaactgc gggctttgca catcaccacc cagacgcaag ttggcactca
     7381 ccatccccgc tgctttttcc ccacgggtaa agatggggtt tatcatcgca aggttgaagt
     7441 tacggatatt gacgttgcca ccaagattac ggcggccttg cggatcggtc acctgcacct
     7501 gtccatcaaa ctggccgtta ttggtcaggc ggatggtcca gcccaattcg gcacggttgt
     7561 tacgcaattc cgccgtcaga ttcagtgtct gaaacgccac cggcagcgcc gcatcgttga
     7621 cggtttgcgt tacctgcacg ttacgccccg aaagggtgat actgccctgc ggcagcccct
     7681 ctttcgtggt gtcccaggca acatccgctt tacccgtgaa gataccgctg gcctgagtgg
     7741 tttctggcat aaacggtttc agcatggcga ggtcgaagcg gttgagattc accaccgcac
     7801 gcccttcggc ccccgcatcg atagtttgcg gcacgcacag ttccgcattc gggttaagcc
     7861 agcagtgtgg cccgatgctg attttttgct ccttattgcg gtaatccagc gcaatatcgc
     7921 gggtcagcga ccacgggccg accggcgtct ggaagcgggt attgctaaga gttcccttcc
     7981 agcgttcttc tttgcgatca aaacttcctg ccagattaag ctgcccggag acaggctcgc
     8041 cctgaatccg caactgtagc tcgtgctgct tttcgctgcc tttggcattc agggtgacga
     8101 ggttgatatt tacatccggc tgcgaaattt gctcaacgcg tacgtcgagt ttcccggcga
     8161 tctgatcggt ggatttaatg tcgccttcca cgcgaacctg cgccacggaa agttcctgcc
     8221 agcgcaggcc gcgcgcggtg atatctgcca gtagttgtgg cgcttccacc gtgccgcgta
     8281 ctttcaccag ccctttcgct gtaccgccaa gccccggcag cgcgttatcc agccccggcg
     8341 cgttgatggt ggcatcaaga ttgagatctt ttacccccag ctcgcctttc acttcggcac
     8401 tgtttggccc gagttccaga tgaagcccag ggatcatcca ctgcatataa ctgttgcctt
     8461 tcagcgtgcc gtcaacgttc actttgttct gtttgacgtt accggtcagc ttcagttctg
     8521 gcacctccat ctgccaggtg ccgccgtaca ggctaccgcg ggttttaatc aagccattga
     8581 gtttcgacgg ccactccggg aactctttgg cggtgtaaat gccattaagc gttagctcac
     8641 cgcgccaact aatggcctgc tgccagtcga gcaacgcctt gagttcagtt ttcccttcca
     8701 gcgccgcgac ggtgagtttg tcgagattga cctgctgttc attacctttg gcatcaaggg
     8761 taatggtggc tggcgggatc tcctgtcctt tcactgccgt acgcatagag agcgtgtaat
     8821 cggtcatttt gccggtaagt ttcagtttca gatcatccgc ctgatactgc ttctcaccag
     8881 tgaacggcca gtaaagctgt ttgctgttca cttccacgtt gagtggcaat ccggcttccg
     8941 ccagtcgcgt ctgggcgcgt aaatccatat ccaccggacc ggaaaggtta acgcctatct
     9001 ccagctgttc gcgcagcgcg ccgcccactt tcagcttcac cttttcacct ttcaacggct
     9061 ccacgttcag tgtgctgttg agggtgatat ccaccggcca gttgtctgac agctgcgccg
     9121 tgccgctggc gttgacgatc ccctggttgg aatcgatatc caaggcgtcc agtttagtat
     9181 tgccgtcaat gctgctcact ttcagcagca tggtgcgcac ggtgatgtcc gtgtcgcccg
     9241 tcacgcgcag ctgctcgccc ttaaactctt caatgttcag gttaagcggc aaatgcacgt
     9301 cggtcatttc cggcaatacc gggcgagaaa agagatcttt cagcgtttcg ccgagcggct
     9361 tttcctccgg ctgcggattt tcaattttcg gttcgaccac ttcttcctgc gccacttcca
     9421 ccactttcgg cagagcaatc agcaggcctt tcagcgacgt cggtttcagg gtcagggttt
     9481 tctcctgcca gttcaggccg gaggtgaagt ccatcaccga caccgtggtg tcatcaatct
     9541 tgatgttgac gttgtccagc gccacccgtg tcagggtgat gggatacggc gtggagagat
     9601 ccagcggacc gctatcttcc tcttcttcaa cctgttcaga aggaggcatt tttttactgt
     9661 cgatgttgac ctgaatgtct ttcagcgcca ggtcattaat acaaacgcta ctgttccaca
     9721 ggcactcaag accgaccgcc agatgcagat tacccgcttt taccgccacg cctagctgct
     9781 cataacgaac gtcagacaag gtgagatcgc gccagccgcc ggtgaccttg ccaatatcca
     9841 gtcctggcac ccagcgatcc gccgctttaa atacaagatg caggccgctg gtggtgccca
     9901 ccagaaacgc caccgatccc agcaacagta agataacgat aaccacgccg aggctgattt
     9961 ttttccataa actcataatt ctggccccag accgatgtaa aactgtaacc cgtgttcgtc
    10021 tttatccgcg accggtacgg caaaatcgag tttgattggc ccgaccggcg attcccagcg
    10081 tacgccgacc ccggtaccgg ttttaaagtc gctgcggcga atatcgctta ccgcttcgcc
    10141 actatcgaca aacaccgcgc cccaccattt tccggtcacg ttgtactggt attccagcga
    10201 tccggttatc aacttcgagg cccctttcag gtcaccgttg gcgtatttcg gggcgataga
    10261 tttgtatttg tagccgcgaa tactgcgatc gcccccgacg aagaaacgca gatccggcgg
    10321 tactttgtcg aaatcaccgg tttcaatcca gcccagcgtg ccgcgtgtca caaagcgatg
    10381 gcgatcgtac agtgtgcgga tccagacgtt ctgcgcctgg aaaacggaga aatcgacatc
    10441 tgaaccccag gccgtgttgg agtagtcgat agagtagcgt tgcgagtctc cccaggttgg
    10501 catcaggcca ccacgagaac gcgtgcggct aatcatcacc ccaggataaa acagcatcgt
    10561 ggtgttggta atttcaccct gggtaaagtg gtcgaggctc cagcgcaggt taatggcacg
    10621 ctgccagccg ctggagagat cccagtagcg agaagccacc agcgtagtgg agtcagattc
    10681 ggtatcgttc aggtcagtgc gcttaaaacc gccctgcacc aaataatatt gttccagtgg
    10741 attcttcagc agcggcattt tatagctgaa gtcgagggtc tgttccggcg cggaaatact
    10801 ggtactggtg gtcaaactgt gaccgtatga gttcatccac ggctttttcc acgtcgcttt
    10861 cacgcgcggt cccacgtccg tagagtaacc gcccccggtt tcgatggtgt tttctgttcg
    10921 cggcgaaacc acgcccgtca atggtaatac tttcgtttcg cgcgctttat caaattgtgg
    10981 agctaccacc accgagttaa accagccggt agcagaaagt cggcggttca gttccgccaa
    11041 atctttcgat tcatactcat cgccctcttt aaacggcacc agattttgca ggtattcatc
    11101 gcggatttgt gatccttcaa aggtcacatg cccaaagcgg taacgttcgc cactgttata
    11161 atcaatatcc cagaaggctt tatgcaggcc gagcgcaatg cccagctgcg ctttggtaaa
    11221 ttcgctatcg aaataacctt tacgcaacgc aatactggtt aaggactttt tgaaattttc
    11281 ataatcgccc tggttcagta ccgtaccaat agccgggcga gtatcgagca atttcaaata
    11341 gtctttatcg gtccgcgcgc cgccgcgcaa taccacatcg gtgccgccaa ttaacaccgg
    11401 cacacctggc gtgactttgg cgatcaatac ctgccgccct ttctttggcg gtggacggag
    11461 atcaaattca atggtcggct ggtaataacc cagcgctttc agaccttcac ggatggcatc
    11521 atcgacgcgt gcgcgaaagc gacggtctgg cgtcacttca tcactttcaa tcgtagaaag
    11581 ctgcgcacga acgttctttt ccagctgtcc cgataacccc tcgacctgta gacggacgtt
    11641 cgcggcgacg gcagatccgc ttaagcagag taagcttaca cagcataact gtcggatata
    11701 gcgcacattt tctcctgaat atcctttttt cctgcccctg gaacgccgtt aaacggctta
    11761 acaaaaatcc agtaatatgg attaaaaagc agactaaacc ccaaatattt cttatgtttt
    11821 actttagacc tattcacggt ggttattgtg tgcaaatacg cctcttgtta caaccttaac
    11881 cccaatgacc gattttcggg agagcgacac catgagttta tttgataaaa agcatctggt
    11941 ttcccccgcc gatgccctgc ctggacgtaa caccccgatg cccgtagcca cgctgcatgc
    12001 ggtcaacggt cactcaatga ccaatgtacc tgacggaatg gagattgcca tttttgcgat
    12061 gggttgtttc tggggtgtgg agcgtctgtt ctggcagtta cccggccttt acagcaccgc
    12121 cgcaggctat accggcggct atacgccaaa tccgacttat cgggaagtgt gctccggtga
    12181 tacgggtcat gccgaagcgg tacgcattgt ttacgatcct tccgtcatca gctatgagca
    12241 gttgctacag gtattttggg agaatcacga tcccgcccag ggaatgcgtc agggcaatga
    12301 ccacggcacg cagtatcgtt cagcgattta tccgctgacc ccagaacagg atgccgcagc
    12361 tcgcgccagt ctggaacgtt ttcaggcggc gatgcttgcc gccgatgacg atcgtcacat
    12421 caccacggaa atcgctaacg ccacaccgtt ttattatgcc gaagatgacc accagcaata
    12481 tctgcataaa aacccatatg gttactgtgg aattggcgga attggcgtct gtctgccgcc
    12541 ggaagcatag cgttacgggt acaaatgtag tttgttgata aagtgctctt tgtctatgcc
    12601 tgatgcggct taaacgactt accaggccta caaaatcgtc caaattcaat atattgcaag
    12661 gactgcgtag gcctgatcgg catagcgcat caggcagtat tgcatttatc agcagtctga
    12721 attttaaccc tctggcgact ttacagtacc ttacgctata ctagcgactg aaaatgccgg
    12781 ttcactttct tcgaatcggc tttcaatgtg tatttcacac aaattaatca actttccctt
    12841 ccgaggatct ggcctgaaag gtcggataag atatgttaaa cagtatttta gtcatactct
    12901 gcttgatcgc tgtaagtgcg ttcttctcga tgtccgagat ctcgcttgcc gcctcacgca
    12961 aaatcaaact taaactgctg gctgatgaag gcaatataaa tgcccaacgc gttctgaata
    13021 tgcaggaaaa tcccggcatg ttctttaccg tggtccaaat cggtctgaac gcagtggcga
    13081 ttctcggcgg tatcgtcggt gatgcggcat tttctccagc ttttcacagc ctgttctccc
    13141 gctatatgtc ggcagagctc tctgagcaac tgagctttat tctctctttc tcgttagtga
    13201 ctggcatgtt tatcctgttt gcggatttaa ccccgaaacg catcggtatg attgcgccag
    13261 aagcggtggc tttgcgtatc atcaatccga tgcgcttctg cctgtacgtt tgcaccccgc
    13321 tggtgtggtt cttcaacggc ctggcgaaca tgatcttccg tattttcaaa ctgccaatgg
    13381 tacgtaaaga tgacatcact tctgatgaca tctacgcggt agtggaagcc ggtgcgctgg
    13441 cgggcgtgtt acgtaaacag gaacacgagc tgattgaaaa cgtctttgag ctggaatccc
    13501 gtaccgttcc gtcttcaatg acaccgcgtg aaaacgtgat ttggtttgat ctccacgaag
    13561 atgagcaaag cctgaagaat aaggtggcgg aacatccgca ctctaagttc ctcgtctgta
    13621 atgaagatat tgaccacatc atcggttatg tcgattctaa agacctgctg aaccgcgtgc
    13681 tggctaacca aagcctggca ctgaacagcg gcgtacaaat tcgcaacacg ctgattgtgc
    13741 cggatacgtt aaccctttca gaggcgttgg aaagttttaa aaccgcaggt gaagacttcg
    13801 cggtgatcat gaacgagtac gcgctggtgg tggggatcat caccctcaac gacgtgatga
    13861 ccacgctgat gggcgatctg gtcggtcagg ggctggaaga gcagattgtc gcccgtgatg
    13921 agaactcatg gctgattgac ggcggcaccc caattgacga cgtcatgcgc gtgctggata
    13981 ttgacgagtt cccgcagtcg ggcaactacg aaaccatcgg cggctttatg atgtttatgc
    14041 tgcgtaagat cccgaaacgc accgattcgg tgaaattcgc cggctacaaa tttgaagtgg
    14101 tggatatcga taactaccgc atcgaccagc tgctggtgac ccggatcgac agcaaggcca
    14161 ccgccctttc gccaaaactg cctgacgcta aagataaaga agaaagcgtc gcgtaacccc
    14221 cagaaacatc aacggctcct gaatcaggag ccgtcttatt actgcatagc actttggtta
    14281 agccatctct gtttgcagac gcataacctg acggttaact tcggacatca ctgacaaatg
    14341 cagcttatcc ttcacttttg ggataagaat cttaccctta tcaaattcaa aagcgccaac
    14401 gtccttgatg tataaccgtc cacggaacag gatctttacg tacttcgcca cctgaagtgg
    14461 gttgtaccgt tggaaaattt tcattgttat ctcctgctga gtattacgcc tgtgcggtgc
    14521 cacaatcggc ccaactatta tgaggcgcaa attttaatgc ttagtgacta tagactatcc
    14581 gggcaatgtt tccaccgcgt ataacttttt ttacctaaag gttacaatta ttcagaatta
    14641 tctttttacc gaagcgcggt cttcagtata agcattcatt tttcatatga tttgtgcgct
    14701 tgaccgcaaa ctggcatcac acttgcggga aattcgataa atagcacata tgattaaaac
    14761 tcagacccaa gtggtcggat cacctgcata tcataagaag gaaacaccat gaccctacgc
    14821 aagattctgg cactcacctg cctgctgttg ccgatgatgg cttccgcaca tcagttcgaa
    14881 atcggtcagc gagtgccgcc gattggcatt accgatcggg gcgagttggt gcttgataaa
    14941 gatcagttta gctacaaaac ctggaacagc gcgcagttag tgggaaaagt gcgagtactg
    15001 caacatattg ctggtcgcac ctctgcaaaa gagaaaaacg cgacgctgat tgaagcgatt
    15061 aaatcagcga agttaccgca cgatcgttac cagaccacca ccattgttaa caccgacgac
    15121 gcgattccgg gttccggcat gtttgtgcgc agcagtctgg agagtaataa aaagctttat
    15181 ccctggtcgc agtttattgt cgatagcaat ggcgtcgcgc gcggtgcctg gcagctggat
    15241 gaagaaagtt ccgctgtagt ggtgctggat aaagacggtc gcgtgcaatg ggccaaagac
    15301 ggggcgctca caccggaaga ggtgcagcaa gtgatggacc tgctgcaaaa gttgctcaaa
    15361 taatggcaat aacataaatt ctggaaaaat aattttcccc tatcgttatc tatgggatag
    15421 gggaattaag tctctgagtt cttacaccga actaatactg gtaacgctag ttgctctttt
    15481 ttaaacgcct caatgatcga tttatatttc tcgcaaatct tttctttggt aatattacta
    15541 tctgcggcca gattcgcact tactatcgca tccacttgtt cggctaattc gacttgttgg
    15601 caacctgcat taaccagaaa attataaata agagagtaat cttcatcctc caaaacaaca
    15661 gaggaaccat tacgaaaacg ttcatcccct ccatagattt taagcgcaaa gcccaaagat
    15721 tctatgacta tggcaaaatt atcatcaatt attttttcgt aaatatacca tccatcgcat
    15781 tctttaattc caccgcctgg tatttggagt tcaaatttac cccgttttaa tttgcttact
    15841 acatcctcca ataaggcata accgaaagat acgtcaccaa acgcctggcg aatataacct
    15901 ttaatccttt ttggtatgtc agactgtttt ttctgcaaaa tatcacaggc at
//
LOCUS       DADMEQ010000088        15929 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.089, whole genome shotgun sequence.
ACCESSION   DADMEQ010000088 DADMEQ010000000
VERSION     DADMEQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.089"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..882)
                     /locus_tag="JNZ05_002819"
     CDS             complement(<1..882)
                     /locus_tag="JNZ05_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY8565988.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGA"
     gene            989..1285
                     /locus_tag="JNZ05_002820"
     CDS             989..1285
                     /locus_tag="JNZ05_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8565989.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1419..1844)
                     /gene="arsC"
                     /locus_tag="JNZ05_002821"
     CDS             complement(1419..1844)
                     /gene="arsC"
                     /locus_tag="JNZ05_002821"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HAY8565990.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1857..3146)
                     /gene="arsB"
                     /locus_tag="JNZ05_002822"
     CDS             complement(1857..3146)
                     /gene="arsB"
                     /locus_tag="JNZ05_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HAY8565991.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3200..3553)
                     /gene="arsR"
                     /locus_tag="JNZ05_002823"
     CDS             complement(3200..3553)
                     /gene="arsR"
                     /locus_tag="JNZ05_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HAY8565992.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            4297..4380
                     /locus_tag="JNZ05_002824"
     CDS             4297..4380
                     /locus_tag="JNZ05_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HAY8565993.1"
                     /translation="MIDKAIIVLGALIVLLELIRFLLQLLN"
     gene            complement(4434..5786)
                     /gene="gorA"
                     /locus_tag="JNZ05_002825"
     CDS             complement(4434..5786)
                     /gene="gorA"
                     /locus_tag="JNZ05_002825"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAY8565994.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5858..6700)
                     /locus_tag="JNZ05_002826"
     CDS             complement(5858..6700)
                     /locus_tag="JNZ05_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HAY8565995.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6903..8945
                     /gene="prlC"
                     /locus_tag="JNZ05_002827"
     CDS             6903..8945
                     /gene="prlC"
                     /locus_tag="JNZ05_002827"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HAY8565996.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8953..9705
                     /gene="rsmJ"
                     /locus_tag="JNZ05_002828"
     CDS             8953..9705
                     /gene="rsmJ"
                     /locus_tag="JNZ05_002828"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HAY8565997.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9754..11223)
                     /gene="dtpB"
                     /locus_tag="JNZ05_002829"
                     /pseudo
     CDS             complement(9754..11223)
                     /gene="dtpB"
                     /locus_tag="JNZ05_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(11529..11963)
                     /gene="uspA"
                     /locus_tag="JNZ05_002830"
     CDS             complement(11529..11963)
                     /gene="uspA"
                     /locus_tag="JNZ05_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HAY8565998.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12354..12689
                     /gene="uspB"
                     /locus_tag="JNZ05_002831"
     CDS             12354..12689
                     /gene="uspB"
                     /locus_tag="JNZ05_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HAY8565999.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12760..14259)
                     /gene="pitA"
                     /locus_tag="JNZ05_002832"
     CDS             complement(12760..14259)
                     /gene="pitA"
                     /locus_tag="JNZ05_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HAY8566000.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            14491..15690
                     /locus_tag="JNZ05_002833"
     CDS             14491..15690
                     /locus_tag="JNZ05_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY8566001.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         4027 a         4057 c         4169 g         3676 t
ORIGIN      
        1 tgcgccggca ctgccgatga ttaatgccat taatgctccc agacccattc cttttgtcat
       61 cagaacagat gccagaggga taaccgcctc agcccggata tacagcggaa taccgacaac
      121 ggcgcttaat gggatggcga agggattatc tgctcctgca tgagcggcaa tccacgctga
      181 gggaataaag ccataaataa aagatcctat taatactcct aacaaaagat atggcagtac
      241 atctttaaac tgttgcaacg catctttggt ggctagtttt atgggattaa tacttactac
      301 agctttggta ctgcaacagg taactacggg gttctcaatg gctttagccg ctggactaca
      361 acaactcact tttatgggcg tatatgtcgt tcccggcgaa gtttttgctg gagcacaaca
      421 atttgctgat gagcttttac tggcaatgat atgacgttca aaccccagag aatccaggat
      481 aatactggca agaacggaga cgccggctgc gataatcgcg tacaacaagg tgactttcca
      541 accaaaggtc atccacatta gtccgacgat aatgggatta agtaatggtg aaacaaataa
      601 aaaagtgaga gtcggaccaa acccggcttt cgctgacaac aatccacgta acatgggtat
      661 tgtcgagcaa ctacagaacg gggtaacggc tcccaacaga gcagccagga gatagcctct
      721 cccttttctg gcccccatca tctgctggat tttatggtct ggcacctttt gtcttatcag
      781 gctgacaccg gcactaatca ctataaacag caacgaaagc tcgacggcga gaaacacaaa
      841 catctctgcg gcatcttgca gcatcgcaag ccaactactc atacgtaacc tcattggata
      901 aatctatatt tccagaataa tcgaaatgaa attcagatca agatatttct ggtattatcg
      961 aaatatcaac cagacagagg ggtgtcatat gcagttggaa gaagtcgcta aagcgctgaa
     1021 agagctaggt cacccgaccc gtttgttcat atttaaacat cttgtcaaag ccggggaaca
     1081 agggttgccg gtcggtgagt tgcaaaagca gcttgggata cctggctcca cactcagcca
     1141 ccatatatca gcactggtat cggtaggact ggtcaaacaa aaccgtgaga gtcggacgct
     1201 gatgtgtgtt tcgcaatacg caatgctgga agcgataatt gaattcttac gcgaggagtg
     1261 ctgcgtaaat agcaaaattg cctgatgcgc tacgctctac atatttcatg ccatatattg
     1321 aatttgcacg gttttgtagg tcggataaga cgcgttagcg tcgcatccga cataaacaaa
     1381 gcgcacttta ccaactatcg gcagtggata tcgccgcttt atttcaggcg cttacccgct
     1441 tcatcaacca ctttctcgcc atcttccttg gagaatgcgc ctttttgcgc atctggcaga
     1501 atttccagca ccacttctga agggcggcac aggcgagttc ccagcggcgt caccacaatc
     1561 gggcgattaa tcagaatcgg gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat
     1621 ttatcttccg caaggcccag ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt
     1681 acggaaatcc ccatatcggc aatgagtttg accagttcat cgcgcgttgg cggagtttcc
     1741 agataatgga taatggtcgg ttctgtgccg ctgttgcgga tcatctccag cgtattacgc
     1801 gacgtgccgc aggccgggtt gtgataaatg gtaatgttgc tcatatcagt atctcattac
     1861 aaagtgaaag agagacgtag cgccagcgca gccagcgtca caaacagcac aggcagggtc
     1921 atgataatcc ctgtacggaa gtaatatccc cagctgatag tcatattctt ctgcgaaagt
     1981 acgtgcagcc agagcagcgt tgccaggcta ccaatcgggg taattttcgg ccccaaatcg
     2041 cagccaatca cgttggcata aaccatcgct tctttgataa cgccagatgc cgtgctgcca
     2101 tcaatggaca acgcgccaac cagtaccgtc ggcatattgt tcataataga agagaggaag
     2161 gcggtgagga atccggtgcc gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg
     2221 agtacgccag aaagatattc cgttaatccg gcattgcgca ggccataaac caccagatac
     2281 atgccgagcg agaagatgac aatctgccag ggggcaccgc gcaggacttt acccgtatta
     2341 atcgcatgac cgcgtttagc gacgacaaat aatatcagtg cgcccacagc tgcaatggcg
     2401 ctcaccggaa tgccgagcgg ttccaggacg aaaaatccca ccagcagaag cagtaaaaca
     2461 acccagccag ttttgaacgt agcaggatct ttgatcgctt ctgcgggaga tttcagcagc
     2521 gccatatcgt agttctgcgg aatatctttg cgaaaataga gatgtagcat caccagcgtg
     2581 gcaacaatcg cggcgatatc caccggcacc atcaccgagg cgtattcgcg aaagccgagg
     2641 ccaaagaaat cagcggaaac gatattcacc aggttggaga caataagcgg caggctggcg
     2701 gtatcggcaa tgaatccggc cgccatcacg aacgccagcg tcgtgccttt actgaatcct
     2761 aaagccagca gcatggcgat gacaatcggc gtcaaaataa gcgccgcgcc atcattggca
     2821 aacagggccg caacggcggc accgagcagg acaatccagg taaacagcaa gcgaccgcgt
     2881 ccattgcccc agcgtgaaac gtgcagcgct gcccattcaa aaaagccgga ctcatccagc
     2941 agcaggctga tgataatgac ggcaataaac gccgccgtcg cgttccagac gatattccac
     3001 accaccggaa tatcacccgg atgaaccacg cccgtaacca acgccagtac cgcgccgagc
     3061 gttgcactcc agccgatgcc taaacctttc ggctgccaga taaccaatac gatggtcagg
     3121 acaaagatag cgcctgccag taacataatg cctcccggat aaaacacatg tgcaaatcca
     3181 tatgtgttta gctaaatttt taactgcaaa tgttcttact gtctccggaa cagttttgtc
     3241 tggccaggtt gcggacaatt gcctgaatct tttcctgttc acatcgccag gcttcatcaa
     3301 taattttcgc cgcccatgct ggaatatgcg gcgataagcg gtaatgaacc cacttacctt
     3361 gcttgcggtc cagcaatagc ccgctttcac gcagcaatgc cagatggcgg gatatcttgg
     3421 gctgcgactg atcgagggca gtgcagagat cgcagacgca taactctccc agttcgctaa
     3481 gcagtaaaac gatgcccaga cgggtttcat cagcaagaat tttgaacaat tggatgggta
     3541 acagaaatga catattgcgc tcctgattgt tgcaggtagt gtctctcttc gaagcggata
     3601 agtcaaaaac atatatgact taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa
     3661 ttttgagttg gctattcatt tgaaaggcgg tttgaatcag gacgtaaaaa aaccgacttt
     3721 gcgtcggttt ttttactctt cagcctgagt tggtggctct ggctggagtg agaatgccca
     3781 catctaacgg ttcaatacta ttagccagtg gctaacattg caagcgttag cgatttcttc
     3841 agctggagaa gggacagttt gctatacatg gtggtgaaag aggatttata tactttcatc
     3901 gggtcgctgc cgtaccaagc cttgtccgaa tccggacgta aaaaaaccga cttcgcgtcg
     3961 gtttttttac tcttcagccc agagttggtg gctctggctg gagtgagaat gccatcatct
     4021 aacgcatcaa tgctaatacc atactgaaac tattgcaagg ttatgctggt tatttagcct
     4081 aattacactg tatgattatc cagttagctc tgaggcattt tcactctggc aatgcgcata
     4141 aacgctttca aagtcctggt cagaagtacg ggtggtgccg ttaactgatg ctctggccgg
     4201 agtgagattg cccacatcta acaatgaggc atgcgccgtg acaggcagtg gatgagtaag
     4261 cggatgcatt ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt
     4321 cttggggcgt taattgtgct gctggaactg atccgctttc tgcttcagct tctgaactga
     4381 tagcggaaac gtaattaagg gctaagagca cactactctt agccctttaa catttaacgc
     4441 atggtcacga actcttctgc cgccgttggg tgaatggcga cggtattgtc gaagtctttt
     4501 ttggttgcgc ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaag
     4561 ccaatgccgt gaataccgac aatcttctct tctggcccaa cgcacaccag cttcatgcgg
     4621 cacggctggc ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact
     4681 ttcacctgat cgtcgccgta ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca
     4741 atcggcggat ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc
     4801 ttgttattaa acaggcgttc agagagacgg cgacccgctg caactgccac cggtgtcagt
     4861 tccactgcac ccgtgttatc gcccaccgcg taaatacctt caatattggt gttttgatat
     4921 ttatcgacga cgatatagcc tttttcgttg gttttaacgc cagcggcttc gaggttgatg
     4981 ttgtcattgg caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga
     5041 ccatcttcca gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg
     5101 gcgttggtat gcaactgtgg gccttcggcg ttcatcactt cgaccagcgt ttcagagatc
     5161 atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg
     5221 aggccgttaa tcacgccagc cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg
     5281 cgctctggca aagcaggaag ggcgaagaag ccatcggaat caataccgta ttccacgcct
     5341 ggaatatccg ggtggctcgg acgaccgccg gtggcgatca gaatatgatc ggccgtgatg
     5401 gtttcgccgt taacttccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca
     5461 tcaacgttat ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg
     5521 gtacggctgg cgatcaacgt ttcccagttg aacttgttga tggtggtatc gaaaccataa
     5581 tcagggccgt acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc
     5641 ggcacgcagc cgacgttcac gcaggtgccg cccagctctt tggcttcaat cagtgcgcat
     5701 ttctggccgt acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg
     5761 gcgatgtaat catagtgttt agtcatgaca tattgtcctt atcgttgatt accgcgattg
     5821 tagcgcgaga cgtaataggt gccagcaatg gctgcaatta ctccggcacg atccagctta
     5881 cggtggcgtg cccggtacct gccggaacca gtttgctgtg cagccacgac agcacgttat
     5941 tcatctgctg ttccagcttc cacggcgggt taatcacaat catgccggaa gcggtcatgc
     6001 cacggcgatc gctgtctggc agcaccgcca gttcaatttg cagaattttg cgaatgccgg
     6061 tcgcttccag gtcgtggatc atgcgcttaa tttgctggcg cagcaccacc ggataccaca
     6121 atgcgtaagt gccagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct
     6181 gatagtcggt tttcatttca tacggcgggt cgataaggat taaaccacgg cgagaaaccg
     6241 gcggcagttt ggccttaagt tgctggaaac cgtcggcttt ttcgacgcgt gcacggctgt
     6301 ctttctgaaa tccagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca
     6361 ggctgtcttg ttcgcgcagt agctggcgag caatcaacgg cgaaccgggg tagtaacgca
     6421 actggccgct acggttgaag tgttttacca cattgatgta cgcctccagt tctgcgggca
     6481 aatcgtcctg ctgccagata cgggcgatac cttcgagata ttcgccggta cgctcggcat
     6541 gttcgctacc taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt
     6601 tatctttctc tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg
     6661 cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa
     6721 gtaaaaattg gcccgtattc ggggcgcaca aaagcctatc aggacagtat accgaaaaga
     6781 ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct
     6841 acacacatta tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc
     6901 taatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg
     6961 aacatgtcgt tccagccgtg actaaggcat tgaacgactg ccgcgaaaat gtggagcgcg
     7021 tagtagcgca aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg
     7081 acgatgtttt ggggcgtatc ttctccccgg tcagccacct gaactcggtg aaaaatagcc
     7141 cggaactgcg tgaagcgtac gaacaaaccc tgccgctgct gtcggaatac agcacctggg
     7201 tagggcaaca tgaagggctg tataaagcgt accgcgacct gcgcgatggc gatcattacg
     7261 ccacgctgaa cacggcgcag aagaaagcgg ttgataacgc cctgcgtgat tttgaactct
     7321 ctggcatagg tctgccgaaa gagaaacagc agcgttacgg cgaaattgct acccgtcttt
     7381 ctgaactggg caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac
     7441 tcgttaccga cgaagcgggg ctggcgggga tgccagaaag cgcgctggct gcggcaaaag
     7501 cccaggccga agcgaaagag ctggaaggct acctgctgac gctggatatc ccaagctacc
     7561 tgccggtaat gacctactgc gacaaccagg ctctgcgcga agagatgtat cgcgcttaca
     7621 gcacccgcgc ctccgatcaa ggcccgaacg ccggtaagtg ggataacagc aaggtgatgg
     7681 aagagatcct cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct
     7741 ttaaatccct tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg
     7801 atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct ggagcaactg cgtgccttcg
     7861 ctaaagccga atttggtgtc gatgagttgc agccgtggga tatcgcttac tacagcgaaa
     7921 aacagaaaca gcacctctac agcatcagcg acgagcagct gcgtccgtac ttcccggaaa
     7981 acaaagcggt taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag
     8041 agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt cttcgaactg tatgacgaaa
     8101 ataacgaact gcgcggcagc ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg
     8161 gggcgtggat ggatggctgc gtaggccaga tgcgtaaagc tgatggttct ctgcaaaaac
     8221 cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca
     8281 ctcacgacga agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga
     8341 cccgcatcga aaccgctggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac
     8401 tgccgagtca gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctctg
     8461 gtcactatga aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga
     8521 agaactacca ggcggcgctg tttattctgc gtcagctgga gttcggcctg tttgatttcc
     8581 gccttcatgc cgagttccgc ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa
     8641 tcaagaaact ggttgccgtg gtgccatctc cgtcctgggg ccgtttcccg cacgctttca
     8701 gccatatttt cgccggtggt tatgcggcag gttactacag ctacctgtgg gctgacgtac
     8761 tggcggcaga tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc
     8821 agtcgttcct cgacaacatt ctgagccgtg gcggttcaga agagccgatg gatctgttca
     8881 aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg
     8941 gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta
     9001 tctgttctgg cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg
     9061 ttaacgccgg aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatcttt
     9121 gttgattttg ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag
     9181 gcggtggcga aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc
     9241 gcagggctgg ggcgcgatgc ctttgtactg gcttcagtcg gctgccgcgt gcggatgctg
     9301 gagcgtaatc cagtggttgc cgcgctactc gacgacggcc tggcgcgtgg ttatgcggat
     9361 gcggaaatcg gcggctggtt gcaggagcgg ttgcagttaa ttcacgcctc cagcctgacg
     9421 gcgctaaccg atattacccc acgcccgcag gtggtctatc tcgatccgat gttcccgcat
     9481 aagcagaaaa gcgcgctggt gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg
     9541 gatcttgatg ccgatggatt actggagcct gcacgcttgc tggcgaccaa acgcgtggtg
     9601 gtaaagcgcc cggactacgc gccaccgctg gcgaatgtcg ccacgccaaa cgcggttgtg
     9661 actaaagggc atcgctttga tatctatgca ggtacgccag tgtaaaagta aagcagggta
     9721 gcgataacgc taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat
     9781 gcgtttcagc cacggcacca tcaacagcat cactactgca acgcccagcg tgaccagacc
     9841 aatcttacca aacacgttgg tatagacggg caacgtctca agcggatcgg taatgttgtc
     9901 cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag
     9961 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
    10021 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
    10081 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagtcgt
    10141 caaaaagccc agtgagcaca taaacatgcc gagagtaaat ttcatcggca tcgagaggtc
    10201 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
    10261 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
    10321 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
    10381 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
    10441 atccagcttg aatgcctgac gaaagaagat gatggtgacg acgatggaga gaacaatcag
    10501 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
    10561 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
    10621 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
    10681 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
    10741 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
    10801 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
    10861 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
    10921 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
    10981 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
    11041 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
    11101 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
    11161 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
    11221 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
    11281 gaaaaagttt acatctgtca gagattctta atattgatgc ggtgaataga ttaatacact
    11341 attacaacag aaaataacca gctgatcaca ctaatgatgg tgttaaattt tgtattggca
    11401 ggagattttg attgcgtacg cagatcgtag gccagataag gggtttacgc tgcatcaggt
    11461 aacaaggcgc gccaaaaagc aaaacgcccg catatgcggg cgttcaggaa cacgtcgtgg
    11521 agggatgatt attcttcttc gtcgcgcagc ggaacaatca gcatatcaac gtgaacggtg
    11581 ttgatcagct gacgtgcgga agacatcagt ttgctccaga agtcctggtg gtgaccacaa
    11641 accaccagat ccatatcgta tttcttgatt gcatcgacca gaacctggcc caggtcgccg
    11701 ctgccgctca gggtttcagt gattggatag cctgcattag tggaaagctc ggtcagcgca
    11761 tgatgtgtct cttcagagat gcgtttctgc atatcaccca gattcacatc aataagcccg
    11821 gtgtataggt cagagtagtt tacatctacg tggatcagag aaactttcgc attgtagggg
    11881 cgagccatag agactgcttt ctctaccaga actttgcttt ccggggagag atcgactgcg
    11941 atgagaatgt gtttataagc catagtgtta ctccttccat aaagttgtcg atgactggcc
    12001 agctagcgtt tcttgtgccg ttacgtcacc cgcgtcctgc gaactacgtg cgcggggcta
    12061 acccagccgt aaagattcag tgttaagacc atccttacct tatagcgacc cggatgatcc
    12121 gtcaatccgc cttgcttacc cgttagataa acaaattttc gacatgaata tattgatagt
    12181 ggttaacctt ctggaaaaaa aacaacctga tctcctacac tatctataga gccgctcgga
    12241 tgttggtcac cacaaaagcg gtttctccag gacctggtgt taactgattg gcagcgtatc
    12301 gggagcggta gccccggggt ggaactccgc gggcaggtcg ccggggagga ggtatgataa
    12361 gcaccgtcgc attattttgg gctttatgtg tcgtttgcat tgttaacatg gcgcgctatt
    12421 tctcatcact acgcgcgttg ttagtggtac tgcgtaactg cgatccattg ctctatcaat
    12481 atgttgatgg agggggcttt tttacctcac atggccaacc caacaaacag gtgcgtctcg
    12541 tttggtatat ctatgcccaa cgttatcgcg atcatcacga tgatgagttt attcgccgct
    12601 gtgagcgggt gcgtcggcag tttattctga ctagcgcatt gtgtggtctg gtggtggtca
    12661 gcctgattgc attgatgatt tggcattaag cacttcagca ataataacgc ataaaaaaag
    12721 cgggccagta aactgacccg ctcgttttgg tgcgtacgat tacaggaact gcaaggagaa
    12781 ccagtacagc ccgccggaaa gtagtaccgc agccggaagg gtaaacaccc aagccatcag
    12841 aatactggtt acggttttac gctgtaagcc gccgccatct accaccatcg tccccgcgac
    12901 agaagaggag agtacgtgag tggtggaaac cggcatcccg gtataactcg ccaggccgat
    12961 tgacactgcc gccgtcatct gggcagacat cccctgagcg taggtcatgc ctttcttacc
    13021 gattttctca ccgatagtcg ttgccacacg acgccagcca atcatcgtac cgatacctaa
    13081 cgccagcgcg accgccatga tgatccacac cggcgcatac tcgatggtgc taagcatgtc
    13141 ggacttcagt ttcttcaaca ggcgctgatc gtcagcactc acaccaggca tcttcaccac
    13201 tttgtcgata gtgtcagaaa cgcacagcat aatgcggcgc atctggctac gttgatcaag
    13261 cgacagcttg tcgtagcttt ccacatcggt ggtcaacata cctttcaggc ggttgagcgc
    13321 gttaatggta ttcgacggat ggcagtggaa ctccgcaggt tgcgttgcgc cagcttccgg
    13381 agccggtact aactgatcag caccggtagc ctgtttgagc agcgcaggat gctgctcaaa
    13441 gtaagcttcg acgttgttga tggcatcacg ggtacgggtg atttcgtagc cagtggcatt
    13501 catgttcacc acgaagcctg ctggcgcgac gccaatcaat accaacataa ccagaccaat
    13561 gcctttctga ccatcgttcg cgccgtgcga aaacgccacg ccgatagcgg aaaggatcag
    13621 cgcgatacgc gtccagaacg gcggcttttt cttgccgtct ttcttttcac gctccgctgg
    13681 ggtcaggtgg atacgggcgc gtttcttggt gccgctccag tagcgacgca gcaagaaaat
    13741 cagaccgcca gcaaacacca ggccgacaat aggggaaacg atcagagaac cgaaaatact
    13801 taatactttc gggatattga gtgcatccac cactgacgtc ccggtcatca acgcattggt
    13861 taaaccaatc ccgatgatcg cgccaatcag cgtatgagag ctggatgcag gtaaaccaaa
    13921 gtaccaggta cccaggttcc agataatcgc cgccagcaac atagagaaca ccatggcaag
    13981 gccatgagac gatcccatat taagcagcag atccgtcggc agcatatgca caatggcata
    14041 ggcaacactc agaccaccca gcaaaacacc caaaaagttg aacaccgccg ccataaccac
    14101 ggcgagctga gaacgcatcg cgcgggtata gataacggtt gccacggcgt tggctgtgtc
    14161 atggaaacca ttgatggctt cgtagaacag cacaaaagcc agtgcaagca ataataacag
    14221 cccggtatgc aaatccaggc cagcaaacaa atgtagcata ggacgttacg ccattttgag
    14281 gacatgaacg cggcgcatta tcagtgactt tcgcggcgcg ggcaaagtga aatatagact
    14341 ttttttgatt tgcctcctgt atggatttca ctcaaaaaat aattatctta tataattcag
    14401 gcaaatactt ccttttagta atattgatgc tggtgcgacc actgaggaat ctttacaatt
    14461 cacgcccgtt ttttctaaga ggagcgcaac gtggaaaggt ttgatgccat tattataggc
    14521 gctggtgcgg cgggtatgtt ctgttctgcg ctggcaggtc aggcaggacg ccgggttctg
    14581 ctgatcgata atggtaaaat actagggcgc aaaatcctta tgtctggcgg tgggcgctgc
    14641 aactttacca acctttatgt cgaaccaggc gcttatctga gccagaatcc gcatttttgt
    14701 aagtctgcac tcgcacgttt tacccagtgg gatttcattg atctggtcaa taaacacggt
    14761 atcgcctggc acgagaaaac gttagggcaa ctcttctgcg atgactccgc gcagcagatt
    14821 gtcgacatgc tggtggatga gtgcgagaag ggcaatgtaa ccttcagatt gcgtagcgaa
    14881 gtgctgagtg tggcgaagga tgaaacaggc ttcacgcttg atctgaacgg catgactgtc
    14941 ggttgcgaaa agctggtcat cgcgaccggt gggctgtcaa tgccggggct gggcgcatcg
    15001 ccgtttggtt ataagattgc cgaacaattt ggcctcaacg tgctgccgac ccgcgcgggt
    15061 ctggtgccat tcactctgca taaaccgttg ctcgaagagt tacaggtgct ggcgggcgtg
    15121 gcggtgcctt ccgtgattac cgctgaaaac ggcaccgttt tccgtgagaa cttactcttc
    15181 acccaccgcg gcttatctgg accggcggtg ttgcagattt caagctactg gcaaccgggt
    15241 gaatttgtca gtatcaatct gctaccggat gtggacctcg aaaccttcct gaatgagcag
    15301 cgtaacgcac atccgaatca aagcctgaaa aacacactgg cggttcatct accgaagcgg
    15361 ttggttgaac gcttacagca actcgggcaa atcccggatg tttcgctaaa acagctcaac
    15421 gtgtgtgacc aacaggcact gattagcaca ttgaccgact ggcgcgtaca acccaacggc
    15481 actgaaggct atcgcactgc cgaagtgacg ctcggcggcg tggacaccaa cgaactttct
    15541 tcacggacga tggaagcgcg caaagtgcct gggctgtact tcatcggcga agtgatggac
    15601 gttaccggct ggctgggggg ctataacttc cagtgggcgt ggtcgagtgc atgggcttgt
    15661 gcgcaggatt tggcgtcaat tgtgaaataa ccatgacgtt ttcccgtcag ccgtttgtat
    15721 atgtcgttat taagtcattt tggtacggtt ctgcatccga cattctggtg ttaactcgct
    15781 gttttgttag cgaattaacg ctattgccgt tgtggttatt ttatagtgta tttattctca
    15841 gagaatatat attactgaaa taaaaaacac aaagaataac caaaggaaat tatttgaaac
    15901 gtaaaaggta tatattttta atctgtaaa
//
LOCUS       DADMEQ010000089        15554 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.090, whole genome shotgun sequence.
ACCESSION   DADMEQ010000089 DADMEQ010000000
VERSION     DADMEQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.090"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            228..422
                     /gene="bfd"
                     /locus_tag="JNZ05_002834"
     CDS             228..422
                     /gene="bfd"
                     /locus_tag="JNZ05_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HAY8566002.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            495..971
                     /gene="bfr"
                     /locus_tag="JNZ05_002835"
     CDS             495..971
                     /gene="bfr"
                     /locus_tag="JNZ05_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HAY8566003.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(968..1435)
                     /locus_tag="JNZ05_002836"
     CDS             complement(968..1435)
                     /locus_tag="JNZ05_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAY8566004.1"
                     /translation="MDTTLPFLILYACLSDLLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1814..2125
                     /gene="rpsJ"
                     /locus_tag="JNZ05_002837"
     CDS             1814..2125
                     /gene="rpsJ"
                     /locus_tag="JNZ05_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAY8566005.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2158..2787
                     /gene="rplC"
                     /locus_tag="JNZ05_002838"
     CDS             2158..2787
                     /gene="rplC"
                     /locus_tag="JNZ05_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAY8566006.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2798..3403
                     /gene="rplD"
                     /locus_tag="JNZ05_002839"
     CDS             2798..3403
                     /gene="rplD"
                     /locus_tag="JNZ05_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAY8566007.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3400..3702
                     /gene="rplW"
                     /locus_tag="JNZ05_002840"
     CDS             3400..3702
                     /gene="rplW"
                     /locus_tag="JNZ05_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAY8566008.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3720..4541
                     /gene="rplB"
                     /locus_tag="JNZ05_002841"
     CDS             3720..4541
                     /gene="rplB"
                     /locus_tag="JNZ05_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAY8566009.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4558..4836
                     /gene="rpsS"
                     /locus_tag="JNZ05_002842"
     CDS             4558..4836
                     /gene="rpsS"
                     /locus_tag="JNZ05_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAY8566010.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4851..5183
                     /gene="rplV"
                     /locus_tag="JNZ05_002843"
     CDS             4851..5183
                     /gene="rplV"
                     /locus_tag="JNZ05_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAY8566011.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5201..5902
                     /gene="rpsC"
                     /locus_tag="JNZ05_002844"
     CDS             5201..5902
                     /gene="rpsC"
                     /locus_tag="JNZ05_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAY8566012.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5915..6325
                     /gene="rplP"
                     /locus_tag="JNZ05_002845"
     CDS             5915..6325
                     /gene="rplP"
                     /locus_tag="JNZ05_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAY8566013.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6325..6516
                     /gene="rpmC"
                     /locus_tag="JNZ05_002846"
     CDS             6325..6516
                     /gene="rpmC"
                     /locus_tag="JNZ05_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAY8566014.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6516..6770
                     /gene="rpsQ"
                     /locus_tag="JNZ05_002847"
     CDS             6516..6770
                     /gene="rpsQ"
                     /locus_tag="JNZ05_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAY8566015.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6935..7306
                     /gene="rplN"
                     /locus_tag="JNZ05_002848"
     CDS             6935..7306
                     /gene="rplN"
                     /locus_tag="JNZ05_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAY8566016.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7317..7631
                     /gene="rplX"
                     /locus_tag="JNZ05_002849"
     CDS             7317..7631
                     /gene="rplX"
                     /locus_tag="JNZ05_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAY8566017.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7646..8185
                     /gene="rplE"
                     /locus_tag="JNZ05_002850"
     CDS             7646..8185
                     /gene="rplE"
                     /locus_tag="JNZ05_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAY8566018.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8200..8505
                     /gene="rpsN"
                     /locus_tag="JNZ05_002851"
     CDS             8200..8505
                     /gene="rpsN"
                     /locus_tag="JNZ05_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAY8566019.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8539..8931
                     /gene="rpsH"
                     /locus_tag="JNZ05_002852"
     CDS             8539..8931
                     /gene="rpsH"
                     /locus_tag="JNZ05_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAY8566020.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8945..9478
                     /gene="rplF"
                     /locus_tag="JNZ05_002853"
     CDS             8945..9478
                     /gene="rplF"
                     /locus_tag="JNZ05_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAY8566021.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9488..9841
                     /gene="rplR"
                     /locus_tag="JNZ05_002854"
     CDS             9488..9841
                     /gene="rplR"
                     /locus_tag="JNZ05_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAY8566022.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9856..10359
                     /gene="rpsE"
                     /locus_tag="JNZ05_002855"
     CDS             9856..10359
                     /gene="rpsE"
                     /locus_tag="JNZ05_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAY8566023.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10363..10542
                     /gene="rpmD"
                     /locus_tag="JNZ05_002856"
     CDS             10363..10542
                     /gene="rpmD"
                     /locus_tag="JNZ05_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAY8566024.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10546..10980
                     /gene="rplO"
                     /locus_tag="JNZ05_002857"
     CDS             10546..10980
                     /gene="rplO"
                     /locus_tag="JNZ05_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAY8566025.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10988..12319
                     /gene="secY"
                     /locus_tag="JNZ05_002858"
     CDS             10988..12319
                     /gene="secY"
                     /locus_tag="JNZ05_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAY8566026.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12351..12467
                     /gene="rpmJ"
                     /locus_tag="JNZ05_002859"
     CDS             12351..12467
                     /gene="rpmJ"
                     /locus_tag="JNZ05_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAY8566027.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12614..12970
                     /gene="rpsM"
                     /locus_tag="JNZ05_002860"
     CDS             12614..12970
                     /gene="rpsM"
                     /locus_tag="JNZ05_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAY8566028.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12987..13376
                     /gene="rpsK"
                     /locus_tag="JNZ05_002861"
     CDS             12987..13376
                     /gene="rpsK"
                     /locus_tag="JNZ05_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAY8566029.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13410..14030
                     /gene="rpsD"
                     /locus_tag="JNZ05_002862"
     CDS             13410..14030
                     /gene="rpsD"
                     /locus_tag="JNZ05_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAY8566030.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14056..15045
                     /gene="rpoA"
                     /locus_tag="JNZ05_002863"
     CDS             14056..15045
                     /gene="rpoA"
                     /locus_tag="JNZ05_002863"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAY8566031.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15086..15469
                     /gene="rplQ"
                     /locus_tag="JNZ05_002864"
     CDS             15086..15469
                     /gene="rplQ"
                     /locus_tag="JNZ05_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAY8566032.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELIDRSEKAEAAAE"
BASE COUNT         4005 a         3655 c         4142 g         3752 t
ORIGIN      
        1 ggccgtaccg ttggcgcggg cgttgttgct aaagttctgg gctaattaca cgttaattag
       61 ttttgaattg aaaagggcgc ttcggcgccc tttttgctgc ccgtaatttg agctcgaaat
      121 atttagtcgt aattttgctc gctgaactat ggcgcaaata aaactgtgct attgtaatca
      181 taaccattct catttacact ttgtgcagaa tttgaacggg agcaatcatg tacgtttgtt
      241 tgtgtaatgg tgtaagtgac aaaaaaattc gccaggcagt ccgtcagttt catccgcaat
      301 ctttccaaca attgcgtaag tttattcctg tgggaaatca atgcggtaag tgcattcgcg
      361 ccgcgcgcga agtgatgcag gacgagttga tgcagatgcc agaatttaaa gagattgcct
      421 gaagcccacg cttatttttt gacatcccgc tagccccatc tacgctctaa tagtggaagc
      481 ggagggacta taaaatgaaa ggtgatgtta aaatcataaa ttatctcaat aaactattgg
      541 gaaatgagct tgtcgcaatc aatcagtatt ttctccatgc ccggatgttt aaaaactggg
      601 gcctgatgcg tctcaatgac gtcgaatacc atgaatccat cgatgagatg aaacacgctg
      661 ataaatatat cgagcgtatt ttgtttttag aaggaatccc caacttacag gatttaggca
      721 aactggggat tggtgaagat gtcgaggaga tgctgcaatc tgacctgcga ttagaactgg
      781 aaggcgcgaa ggatctgcgt gaggctatcg catatgccga cagcgttcat gactatgtta
      841 gccgcgatat gatgatcgag atccttgctg atgaagaagg tcatatcgac tggctcgaaa
      901 ctgaactgga tctcatcggt aaaatcggcc tacaaaatta cctgcaatca caaattaaag
      961 ttaaagatta acggcctatc agcaaactat cccagcctat aacgaaaccc gcagccgcca
     1021 gaaatggccc aaaaggcagc gggtttttta atgattcttt tcctctcatg agcagaccaa
     1081 taactacggc tccgcaagcg aatgaggcag caaggaaaac cagtcgtggt aagaaggccc
     1141 atgaatgcca ggcgccaagg gcggcgagaa acttcacatc accatagcct aatccttctt
     1201 tatgtcgcag tatgcgatac ccccagtaaa tgacagcgaa tgtgccgtaa ccaacaattg
     1261 ccccccataa cgcatcggct aaaccatcag gatgacaaac ttgatagaac aaaagacctg
     1321 accagagtaa cgggcacgta aatctgtcag gtaacaagcc atgctttgca tcccagaaaa
     1381 aaagtaggtc tgataaacaa gcgtacaaaa ttagaaaggg aagggtagtg tccataaaac
     1441 atcctctgta attggagtat caccatcgaa taactccgga tgctcagcag cagagagagc
     1501 gctgatttgt taacctgatt ccggtgaaaa acgctatctt cacaacctta caaatttcgt
     1561 ggaatgcagc tcgcaatctg cgcgttgcga ttaatttcta cccgatgctt gcgtcaggct
     1621 cagatttgcg tataatgcgc gggcttgtcg tagttgacag caggttcaat ctgaacctca
     1681 gttagccgaa tttggctacc taacaatgct cccaatcggg gagctacgta agaacggtta
     1741 cactctccca tcaatcgtaa tgggtctgag gagtaatcat tttcgtttat aaaataattg
     1801 gagctctggt ctcatgcaga accaaagaat ccgtatccgc ctgaaagcgt ttgatcatcg
     1861 tctgatcgat caagcaaccg cggaaatcgt cgagactgcc aagcgcactg gtgcgcaggt
     1921 ccgtggtccg atcccgctgc cgacacgcaa agagcgcttc actgttctga tctccccgca
     1981 cgtcaacaaa gacgcgcgcg atcagtacga aatccgtact cacttgcgtc tggttgacat
     2041 cgttgagcca accgagaaaa ccgttgatgc tctgatgcgt ctggatctgg ctgccggtgt
     2101 agacgtgcag atcagcctgg gttaatcagg tcattgagcg attgagaggt tgaaacaatg
     2161 attggtttag tcggtaaaaa agtgggtatg acccgtatct tcacagaaga cggcgtttct
     2221 atcccagtaa ccgtaatcga agttgaagca aaccgcgtta ctcaggttaa agacctggct
     2281 aacgatggct accgtgctat tcaggtgacc accggtgcta aaaaagctaa ccgtgtgacc
     2341 aagcctgaag ctggccactt cgctaaagct ggcgtagaag ctggccgtgg tctgtgggaa
     2401 ttccgcctgg ctgaaggcga agagttcact gtaggtcaga gcattagcgt tgaactgttt
     2461 gctgacgtta aaaaagttga cgtaactggc acctctaaag gtaaaggttt cgcaggtacc
     2521 gttaagcgct ggaacttccg tacccaggac gctactcacg gtaactcctt gtctcaccgc
     2581 gttccgggtt ctatcggtca gaaccagact ccgggcaaag tgttcaaagg caagaaaatg
     2641 gcaggtcaga tgggtaacga acgtgtaacc gttcagagcc ttgacgtagt acgcgttgac
     2701 gctgagcgca acctgctgct ggttaaaggt gctgtcccgg gtgcaaccgg tagcgacctg
     2761 atcgttaaac cagctgtgaa ggcgtaagga gatagcaatg gaattagtat tgaaagacgc
     2821 gcagagcgcg ctgactgttt ccgaaactac cttcggtcgt gatttcaacg aagcgctggt
     2881 tcaccaggtt gttgttgctt atgcagctgg tgctcgtcag ggtactcgtg ctcagaagac
     2941 tcgtgctgaa gtaactggtt ccggtaaaaa accgtggcgc cagaaaggca ccggccgtgc
     3001 gcgttctggt tctatcaaga gcccgatctg gcgttctggt ggcgtgacct ttgctgctcg
     3061 tccgcaggac cacagtcaaa aagttaacaa gaagatgtac cgcggcgcgc tgaaaagcat
     3121 cctgtccgaa ctggtacgtc aggatcgtct gatcgttgtc gagaagttct ctgtagaagc
     3181 gccgaaaact aagctgctgg cacagaaact gaaagacatg gctctggaag atgtgctgat
     3241 catcaccggt gagctggacg aaaacctgtt cctggctgcg cgcaacctgc acaaggttga
     3301 cgtacgcgat gcaactggta tcgacccggt tagcctgatc gccttcgaca aagtcgtaat
     3361 gactgctgat gctgttaagc aagttgagga gatgctggca tgattcgtga agaacgtctg
     3421 ctgaaggtgc tgcgtgcacc gcacgtttct gaaaaagcgt ctactgcgat ggaaaaatcc
     3481 aacaccatcg tactcaaagt tgctaaagac gcgaccaaag cagaaatcaa agctgctgtg
     3541 cagaaactgt ttgaagtcga agtcgaagtc gttaacaccc tggtagttaa agggaaagtt
     3601 aaacgtcacg gacagcgtat cggtcgtcgt agcgactgga aaaaagctta cgtcaccctg
     3661 aaagaaggcc agaatctgga cttcgttggc ggcgctgagt aagtcggagg agtaatacaa
     3721 tggcagttgt taaatgtaaa ccgacatctc cgggtcgtcg ccacgtagtt aaagtggtta
     3781 accctgagct gcacaagggc aaaccttttg ctccgttgct ggaaaaaaac agcaaatccg
     3841 gtggtcgtaa caacaatggc cgtatcacca ctcgtcatat cggtggtggc cacaagcagg
     3901 cttaccgtat tgttgacttc aaacgcaaca aagacggtat cccggcagtt gttgaacgtc
     3961 ttgagtacga tccgaaccgt tccgcgaaca tcgcgctggt tctgtacaaa gacggtgaac
     4021 gccgttacat cctggcccct aaaggcctga aagctggcga ccagattcag tctggcgttg
     4081 atgctgcaat caaaccaggt aacaccctgc cgatgcgcaa catcccggtt ggttctactg
     4141 ttcataacgt agaaatgaaa ccaggtaaag gcggtcagct ggcacgttcc gctggtactt
     4201 acgttcagat cgttgctcgt gatggtgctt atgtcaccct gcgtctgcgt tctggtgaaa
     4261 tgcgtaaagt agaagcagac tgccgtgcaa ctctgggcga agttggcaat gctgagcata
     4321 tgctgcgcgt tctgggtaaa gcaggtgctg cacgctggcg tggtgttcgt ccgaccgttc
     4381 gcggtactgc gatgaacccg gtagaccacc cacatggtgg tggtgaaggt cgtaactttg
     4441 gtaagcaccc ggtaactccg tggggcgttc agaccaaagg taagaagacc cgcagcaaca
     4501 agcgtactga taaattcatc gtacgtcgcc gtagcaaata attttagagg ataagccatg
     4561 ccacgttctc tcaagaaagg tccttttatt gacctgcact tgctgaagaa ggtagagaaa
     4621 gcggtggaaa gcggagacaa gaagcccctg cgcacttggt cccgtcgttc aacgatcttt
     4681 cctaacatga tcggtttgac catcgctgtc cataatggtc gtcagcacgt tccggtattt
     4741 gtaaccgacg aaatggtcgg tcacaaactg ggtgaattcg caccgactcg tacttatcgc
     4801 ggccacgctg ctgataaaaa agcgaagaag aaataaggta ggaggaagag atggaaacta
     4861 ttgctaaaca tcgccatgct cgttcttctg ctcagaaggt tcgccttgtt gctgacctga
     4921 ttcgcggtaa gaaagtgtcg caggctctgg atattttgac ctacaccaac aagaaagcgg
     4981 ctgtacaggt caagaaagtt ctggaatctg ccattgctaa cgctgaacac aacgatggcg
     5041 ctgacattga cgatctgaaa gttacgaaaa ttttcgtaga cgaaggcccg agcatgaagc
     5101 gcattatgcc gcgtgcaaaa ggtcgtgcag atcgtatcct gaagcgcacc agccacatca
     5161 ctgtggttgt gtccgatcgc tgagactctg gagactagca atgggtcaga aagtacatcc
     5221 taatggtatt cgcctgggta ttgtaaaacc atggaactct acctggtttg cgaacaccaa
     5281 agaattcgct gacaacctgg acagcgattt taaagtacgt cagtacctga ctaaggaact
     5341 ggctaaagcg tccgtatctc gtatcgttat cgagcgtccg gctaagagca tccgtgtaac
     5401 cattcacact gctcgcccgg gtatcgttat cggtaagaaa ggtgaagacg tagaaaaact
     5461 gcgtaaggtc gtagcggaca tcgctggcgt tcctgcacag atcaacatcg ccgaagttcg
     5521 taagcctgaa ctggacgcaa aactggttgc tgacagcatc acttctcagc tggaacgtcg
     5581 cgttatgttc cgtcgtgcta tgaagcgtgc tgtacagaac gcaatgcgtc tgggcgctaa
     5641 aggtattaaa gttgaagtta gcggccgtct gggcggcgcg gaaatcgcac gtaccgaatg
     5701 gtaccgcgaa ggtcgcgtac cgctgcacac tctgcgtgct gacatcgact acaacacctc
     5761 tgaagcgcac accacttacg gtgtaatcgg cgttaaagtg tggatcttca aaggcgagat
     5821 cctgggtggt atggctgctg ttgaacaacc ggaaaaaccg gctgctcagc ctaaaaagca
     5881 gcagcgtaaa ggccgtaaat aaggagcgtc gctgatgtta caaccaaagc gtacaaaatt
     5941 ccgtaaaatg cacaaaggcc gtaaccgcgg tctggcgcag ggtacggatg ttagcttcgg
     6001 cagcttcggt ctgaaagctg ttggccgtgg tcgtctgact gcccgtcaga tcgaagcagc
     6061 acgtcgtgct atgacccgtg cagttaagcg tcaaggtaag atctggatcc gtgtgttccc
     6121 ggacaaaccg atcactgaaa agccgctggc agtgcgtatg ggtaaaggta aaggtaacgt
     6181 ggagtattgg gttgccttga ttcagccggg taaagtcctg tatgaaatgg acggtgttcc
     6241 ggaagagctg gcccgtgaag cattcaagct ggcagcagcg aaactgccga ttaaaaccac
     6301 ctttgtaact aagacggtga tgtaatgaaa gcaaaagagc tgcgtgagaa gagcattgaa
     6361 gagctgaaca ccgagctgct gaacctgctg cgtgagcagt tcaacctgcg tatgcaggct
     6421 gcaagtggcc agctgcaaca gtctcacctg ttgaagcaag tgcgtcgcga tgtcgcacgc
     6481 gttaagactt tactgaacga gaaggcgggt gcgtaatgac cgataaaatc cgtactctgc
     6541 aaggtcgcgt tgttagcgac aaaatggaga aatccattgt tgttgctatc gaacgttttg
     6601 tgaaacaccc gatctacggt aaattcatca agcgtacgac caaactgcac gtacatgacg
     6661 agaacaacga atgcggtatc ggtgacgtgg ttgaaatccg cgaatgccgt ccgctgtcca
     6721 agactaaatc ctggacgctg gttcgcgttg tagagaaagc ggttctgtaa tacagtacac
     6781 tctctcgata cgaataaacg gctcagaaat gagccgttta ttttttctac ccatatcctt
     6841 gaagcggtgt tataatgccg cgccctcgat atggggattt ttaacgacct gattttcggg
     6901 tctcagtagt agttgacatt agcggagcac taaaatgatc caagaacaga ctatgctgaa
     6961 cgtcgccgac aactccggtg cacgtcgcgt aatgtgtatc aaggttctgg gtggctcgca
     7021 ccgtcgctac gcaggcgtag gcgacatcat caagatcacc atcaaagaag caattccgcg
     7081 tggtaaggtc aaaaaaggtg atgtgctgaa ggcggtagtg gtgcgcacca agaagggtgt
     7141 tcgtcgcccg gacggttctg tcattcgctt cgatggtaat gcttgtgttc ttctgaacaa
     7201 caacagcgag cagcctatcg gtacgcgtat ttttgggccg gtaactcgtg agcttcgtag
     7261 tgagaagttc atgaaaatta tctctctggc accagaagta ctctaaggag cgaatcatgg
     7321 cagcgaaaat ccgtcgtgat gacgaagtta tcgtgttaac cggtaaagat aaaggtaaac
     7381 gcggtaaagt taagaatgtc ctgtcttccg gcaaggtcat tgttgaaggt atcaacctgg
     7441 ttaagaaaca tcagaagccg gttccggccc tgaaccaacc gggtggcatc gttgaaaaag
     7501 aagccgctat tcaggtttcc aacgtagcaa tcttcaatgc ggcaaccggc aaggctgacc
     7561 gtgtaggctt tagattcgaa gacggcaaaa aagtccgttt cttcaagtct aacagcgaaa
     7621 ctatcaagta atttggagta gtacgatggc gaaactgcat gattactaca aagacgaagt
     7681 agttaaaaaa ctcatgactg agtttaacta caattctgtc atgcaagtcc ctcgggtcga
     7741 gaagatcacc ctgaacatgg gtgttggtga agcgatcgct gacaaaaaac tgctggataa
     7801 cgcagcagct gacctggcag caatctccgg tcaaaaaccg ctgatcacca aagcacgcaa
     7861 atctgttgca ggcttcaaaa tccgtcaggg ctatccgatc ggctgtaaag taactctgcg
     7921 tggcgaacgc atgtgggagt tctttgagcg cctgatcact attgctgtac ctcgtatccg
     7981 tgacttccgt ggcctgtccg ctaagtcttt cgacggtcgt ggtaactaca gcatgggtgt
     8041 ccgtgagcag atcatcttcc cagaaatcga ctacgataaa gtcgaccgcg ttcgtggttt
     8101 ggatattacc attaccacta ctgcgaaatc tgacgaagaa ggccgcgctc tgctggctgc
     8161 ctttgacttc ccgttccgca agtaaggtag ggttactaaa tggctaagca atcaatgaaa
     8221 gcacgcgaag taaaacgcgt agctttagct gataaatact tcgcgaaacg cgctgaactg
     8281 aaagcgatca tctctgatgt gaacgcttcc gacgaagatc gttggaacgc tgttctcaag
     8341 ctgcagactc tgccgcgtga ttccagcccg tctcgtcagc gtaaccgctg ccgtcaaaca
     8401 ggtcgtccgc atggtttcct gcggaagttc gggttgagcc gtattaaggt ccgtgaagcc
     8461 gctatgcgcg gtgaaatccc gggtctgaaa aaggctagct ggtaattgtc accaattgaa
     8521 tcacgggagt aaagacagat gagcatgcaa gatccgatcg cggatatgct gacccgtatc
     8581 cgtaacggtc aggccgcgaa caaagctgcg gtcaccatgc cttcctccaa gctgaaagtg
     8641 gcaatcgcca acgtgctgaa ggaagaaggt tttattgaag attttaaagt tgaaggcgac
     8701 accaagcctg aactggaact tactctgaag tatttccagg gcaaagctgt tgtagaaagc
     8761 attcagcgtg tcagccgccc aggtctgcgc atctataaac gtaaagatga gctgccgaaa
     8821 gttatggcgg gtctgggtat cgcagttgtt tctacctcta aaggtgttat gactgatcgt
     8881 gcagcgcgcc aggctggtct tggtggcgaa attatctgct acgtagccta aatcggagga
     8941 aaaaatgtct cgtgttgcta aagcaccggt cgttgttcct gccggcgttg acgtaaaaat
     9001 caacggtcag gttattacga tcaaaggtaa aaacggcgag ctgactcgta ctctcaacga
     9061 tgctgttgaa gttaaacatg cagataatac cctgaccttc ggtccgcgtg atggttacgc
     9121 agacggttgg gcacaggctg gtaccgcgcg tgccctgctg aactcaatgg ttatcggtgt
     9181 taccgaaggc ttcactaaga agctgcagct ggttggtgta ggttaccgtg cagcggttaa
     9241 aggcaatgtg attaacctgt ctctgggttt ctctcatcct gttgaccatc agctgcctgc
     9301 gggtatcact gctgaatgtc cgactcagac tgaaatcgtg ctgaaaggcg ctgataagca
     9361 ggtgatcggc caggttgcag cggatctgcg cgcctaccgt cgtcctgagc cttacaaagg
     9421 caagggtgtt cgttacgccg acgaagtcgt gcgtaccaaa gaggctaaga agaagtaagg
     9481 taacactatg gataagaaat ctgctcgtat ccgtcgtgcg acccgcgcac gccgcaagct
     9541 ccaggagctg ggcgcaactc gcctggtggt acatcgtacc ccgcgtcaca tttacgcaca
     9601 ggtaattgca ccgaacggtt ctgaagttct ggtagctgct tctactgtag aaaaagctat
     9661 cgctgaacaa ctgaagtaca ccggtaacaa agacgcggct gcagctgtgg gtaaagctgt
     9721 cgctgaacgc gctctggaaa aaggcatcaa agatgtatcc tttgaccgtt ccgggttcca
     9781 atatcatggt cgtgtccagg cactggcaga tgctgcccgt gaagctggcc ttcagttcta
     9841 aggtagaggt gtaagatggc tcacatcgaa aaacaagctg gcgaactgca ggaaaagctg
     9901 atcgcggtaa accgcgtatc taaaaccgtt aaaggtggtc gtattttctc cttcacagct
     9961 ctgactgtag ttggcgatgg taacggtcgc gttggttttg gttacggtaa agcgcgtgaa
    10021 gttccagcag cgatccagaa agcgatggaa aaagcccgtc gcaatatgat taacgtcgcg
    10081 ctgaataacg gcactctaca acaccctgtt aaaggtgttc acacgggttc tcgcgtattc
    10141 atgcagccgg cttccgaagg taccggtatc atcgccggtg gtgcaatgcg cgccgttctg
    10201 gaagtcgctg gggttcataa cgttctggct aaagcatatg gttccaccaa cccgatcaac
    10261 gtggttcgtg caactattga tggcctagaa aatatgaatt ctccagaaat ggtcgctgcc
    10321 aagcgtggta aatccgttga agaaattctg gggaaataaa ccatggcaaa gactattaaa
    10381 attactcaaa cccgcagtgc aatcggtcgt ctgccgaaac acaaggcaac gctgcttggc
    10441 ttgggtctgc gtcgtattgg tcacaccgta gagcgcgagg atactcctgc tattcgcggt
    10501 atgatcaacg cggtttcctt catggttaaa gttgaggagt aagagatgcg tttaaatact
    10561 ctgtctccgg ccgaaggctc caaaaaggcg ggtaaacgcc tggggcgtgg tatcggttct
    10621 ggcctcggta aaaccggtgg tcgtggtcac aaaggtcaga agtctcgttc tggcggtggc
    10681 gtacgtcgcg gtttcgaggg tggtcagatg cctctgtacc gtcgtctgcc gaaattcggc
    10741 ttcacttctc gtaaagcagc gattacagcc gaagttcgtc tatctgacct ggctaaagta
    10801 gaaggcggtg ttgtagacct gaacacgctg aaagcggcta acattatcgg tatccagatc
    10861 gagttcgcga aagtgatcct ggctggcgaa gtaacgactc cggtaactgt tcgtggcctg
    10921 cgtgttacta aaggcgctcg tgctgctatc gaagctgctg gcggtaaaat cgaggaataa
    10981 gtagcagatg gctaaacaac cgggattaga ttttcaaagt gccaaaggtg gcttaggcga
    11041 gctgaaacgc agactgctgt ttgttatcgg tgcgctgatt gtgttccgta ttggctcttt
    11101 tattccgatc cctggtattg atgccgctgt acttgccaaa ctgcttgagc aacagcgagg
    11161 caccatcatt gagatgttta acatgttctc tggtggtgct ctcagccgtg cttctatctt
    11221 tgctctgggg atcatgccgt atatttcggc gtcgatcatt atccagctgc tgacggtggt
    11281 tcacccaacg ttggcagaaa ttaagaaaga aggggagtct ggtcgtcgta agatcagcca
    11341 gtacacccgt tacggtactc tggtgctggc aatattccag tcgatcggta ttgctaccgg
    11401 tctgccgaat atgcctggta tgcaaggcct ggtgattaac ccgggctttg cattctactt
    11461 caccgctgtt gtaagtctgg tcacaggaac gatgttcctg atgtggttgg gcgaacagat
    11521 cactgaacga ggtatcggca acggtatttc aatcattatc ttcgccggta ttgtcgcggg
    11581 actcccgcca gccattgccc atactatcga gcaagcgcgt caaggcgacc tgcacttcct
    11641 cgtgttgctg ttggttgcag tattagtatt tgcagtgacg ttctttgttg tgtttgttga
    11701 gcgtggtcaa cgccgcattg tggtaaacta cgcgaaacgt cagcaaggtc gtcgtgtcta
    11761 tgctgcacag agcacacatt taccgctgaa agtgaatatg gcgggggtaa tcccggcaat
    11821 cttcgcttcc agtattattc tgttcccggc gaccatcgcg tcatggttcg ggggcggtac
    11881 tggttggaac tggctgacaa caatttcgct gtatttgcag cctgggcaac cgctttatgt
    11941 gttactctat gcgtctgcaa tcatcttctt ctgtttcttc tacacggcgt tggttttcaa
    12001 cccgcgtgaa acagcagata acctgaagaa gtccggtgca tttgtaccag gaattcgtcc
    12061 gggagagcaa acggcgaagt atatcgataa agtaatgacc cgcctgacct tggttggtgc
    12121 gctgtatatt acctttatct gcctgatccc ggagttcatg cgtgatgcaa tgaaagtacc
    12181 gttctacttc ggtgggacct cactgcttat cgttgttgtc gtgattatgg actttatggc
    12241 tcaagtgcaa actctgatga tgtccagtca gtatgagtct gcattgaaga aggcgaacct
    12301 gaaaggctac ggccgataat tggtcgcccg agaagttacg gagagtaaaa atgaaagttc
    12361 gtgcttccgt caagaaatta tgccgtaact gcaaaatcgt taagcgtgat ggtgtcatcc
    12421 gtgtgatttg cagtgccgag ccgaagcata aacagcgcca aggctgattt tttcgcatat
    12481 ttttcttgca aagttgggtt gagctggcta gattagccag ccaatctttt gtatgtctgt
    12541 gcgtttccat ttgagtatcc tgaaaacggg cttttcagca tggaacgtac atattaaata
    12601 gtaggagtgc atagtggccc gtatagcagg cattaacatt cctgatcata agcatgccgt
    12661 aatcgcatta acttcgattt atggcgtcgg caagacccgt tctaaagcca tcctggctgc
    12721 agcgggtatc gctgaagatg ttaagatcag tgagctgtct gaaggacaaa tcgacacgct
    12781 gcgtgacgaa gttgccaaat ttgtcgttga aggtgatctg cgccgtgaaa tcagcatgag
    12841 catcaagcgc ctgatggatc ttggttgcta tcgcggtttg cgtcatcgtc gtggtctccc
    12901 ggttcgcggt cagcgtacca agaccaacgc acgtacccgt aagggtccgc gcaaaccgat
    12961 caagaaataa tcggggtgat tgaataatgg caaaggcacc aattcgtgca cgtaaacgtg
    13021 taagaaaaca agtctctgac ggcgtggctc atatccatgc ttctttcaac aacaccatcg
    13081 tgactatcac tgatcgtcag ggtaacgcgt tgggttgggc aacagccggt ggttccggtt
    13141 tccgtggttc tcgcaaatcc actccgtttg cagctcaggt tgcagcagag cgttgcgctg
    13201 acgccgtgaa agaatacggc atcaagaatc tggaagttat ggttaaaggt ccgggtccag
    13261 gccgcgaatc tactattcgt gctctgaacg ccgcaggttt ccgcatcact aacattactg
    13321 atgtgactcc gatccctcat aacggttgtc gtccgccgaa aaaacgtcgc gtataacgcc
    13381 tcgttttcca ggtttgttgg agaaagaaaa tggcaagata tttgggtcct aagctcaagc
    13441 tgagccgtcg tgagggcacc gacttattcc ttaagtctgg cgttcgcgcg atcgatacca
    13501 agtgtaaaat tgaacaagct cctggccagc acggtgcgcg taaaccgcgt ctgtctgact
    13561 atggtgtgca gttgcgtgaa aagcaaaaag ttcgccgtat ctatggtgtg ctggagcgtc
    13621 agttccgtaa ctactacaaa gaagcagcac gtctgaaagg caacaccggt gaaaacctgt
    13681 tggctctgct ggaaggtcgt ctggacaacg ttgtataccg tatgggcttc ggtgccactc
    13741 gtgcagaagc acgtcagctg gttagccata aagcaattat ggtaaacggt cgtgttgtta
    13801 acatcgcttc ttatcaggtt agtccgaatg acgttgtaag cattcgtgag aaagcgaaga
    13861 agcagtctcg cgtgaaagcc gctctggagc tggctgagca gcgtgaaaag ccaacttggc
    13921 tggaagttga tgctggcaag atggaaggta cgtttaagcg taagccggag cgttctgatc
    13981 tgtctgcgga cattaacgaa cacctgatcg tcgagcttta ctccaagtaa agcttagtac
    14041 caaagagagg acacaatgca gggttctgtg acagagtttc taaaaccgcg cctggttgat
    14101 atcgagcaag tgagttcgac gcacgccaag gtgacccttg agcctttaga gcgtggcttt
    14161 ggccatactc tgggtaacgc actgcgccgt attctgctct catcgatgcc gggttgcgcg
    14221 gtgaccgagg ttgagattga tggtgtacta catgagtaca gcaccaaaga aggcgttcag
    14281 gaagatatcc tggaaatcct gctcaacctg aaagggctgg cggtgagagt tcagggcaaa
    14341 gatgaagtta ttcttacctt gaataaatct ggcattggcc ctgtgactgc agccgatatc
    14401 acccacgacg gtgatgtcga aatcgtcaag ccgcagcacg tgatctgcca cctgaccgat
    14461 gagaacgcgt ctattagcat gcgtatcaaa gttcagcgcg gtcgtggtta tgtgccggct
    14521 tctacccgaa ttcattcgga agaagatgag cgcccaatcg gccgtctgct ggtcgacgca
    14581 tgctacagcc ctgtggagcg tattgcctac aatgttgaag cagcgcgtgt agaacagcgt
    14641 accgacctgg acaagctggt catcgaaatg gaaaccaacg gcacaatcga tcctgaagag
    14701 gcgattcgtc gtgcggcaac cattctggct gaacaactgg aagctttcgt tgacttacgt
    14761 gatgtacgtc agcctgaagt gaaagaagag aaaccagagt tcgatccgat cctgctgcgc
    14821 cctgttgacg atctggaatt gactgtccgc tctgctaact gccttaaagc agaagctatc
    14881 cactatatcg gtgatctggt acagcgtacc gaggttgagc tccttaaaac gcctaacctt
    14941 ggtaaaaaat ctcttactga gattaaagac gtgctggctt cccgtggact gtctctgggc
    15001 atgcgcttgg aaaactggcc accggcaagc atcgctgacg agtaaccgga tcacaggtta
    15061 aggttttact gagaaggata aggtcatgcg ccatcgtaag agtggtcgtc aactgaaccg
    15121 caacagcagc catcgccagg ctatgttccg caatatggca ggttcactgg ttcgtcatga
    15181 aatcatcaag acgactctgc ctaaagcgaa agagctgcgc cgcgtagttg agccgctgat
    15241 tactcttgcc aagactgata gcgttgctaa tcgtcgtctg gcattcgccc gtactcgtga
    15301 taacgagatc gtggcaaaac tgtttaacga actgggcccg cgtttcgcga gccgtgccgg
    15361 tggttacact cgtattctga agtgtggctt ccgtgcaggc gacaacgcgc cgatggctta
    15421 catcgagctg attgatcgtt cagagaaagc agaagctgct gcagagtaat ctgaagcaac
    15481 gtaaaaaaac ccgccccggc gggttttttt atacccgtag tatccccact tatctacaat
    15541 agctgtactc tttt
//
LOCUS       DADMEQ010000090        15458 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.091, whole genome shotgun sequence.
ACCESSION   DADMEQ010000090 DADMEQ010000000
VERSION     DADMEQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15458
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.091"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(283..987)
                     /locus_tag="JNZ05_002865"
     CDS             complement(283..987)
                     /locus_tag="JNZ05_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY8566033.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGLGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1124..1576)
                     /gene="moaE"
                     /locus_tag="JNZ05_002866"
     CDS             complement(1124..1576)
                     /gene="moaE"
                     /locus_tag="JNZ05_002866"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HAY8566034.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(1578..1823)
                     /gene="moaD"
                     /locus_tag="JNZ05_002867"
     CDS             complement(1578..1823)
                     /gene="moaD"
                     /locus_tag="JNZ05_002867"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HAY8566035.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFLPPVTGG"
     gene            complement(1816..2301)
                     /gene="moaC"
                     /locus_tag="JNZ05_002868"
     CDS             complement(1816..2301)
                     /gene="moaC"
                     /locus_tag="JNZ05_002868"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HAY8566036.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2304..2816)
                     /gene="moaB"
                     /locus_tag="JNZ05_002869"
     CDS             complement(2304..2816)
                     /gene="moaB"
                     /locus_tag="JNZ05_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HAY8566037.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2838..3827)
                     /gene="moaA"
                     /locus_tag="JNZ05_002870"
     CDS             complement(2838..3827)
                     /gene="moaA"
                     /locus_tag="JNZ05_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HAY8566038.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3815..3959)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4224..5132
                     /gene="yvcK"
                     /locus_tag="JNZ05_002871"
     CDS             4224..5132
                     /gene="yvcK"
                     /locus_tag="JNZ05_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HAY8566039.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5324..7345)
                     /gene="uvrB"
                     /locus_tag="JNZ05_002872"
     CDS             complement(5324..7345)
                     /gene="uvrB"
                     /locus_tag="JNZ05_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HAY8566040.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7924..8601)
                     /gene="bioD"
                     /locus_tag="JNZ05_002873"
     CDS             complement(7924..8601)
                     /gene="bioD"
                     /locus_tag="JNZ05_002873"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY8566041.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPSSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QVIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8594..9349)
                     /gene="bioC"
                     /locus_tag="JNZ05_002874"
     CDS             complement(8594..9349)
                     /gene="bioC"
                     /locus_tag="JNZ05_002874"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HAY8566042.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9336..10490)
                     /gene="bioF"
                     /locus_tag="JNZ05_002875"
     CDS             complement(9336..10490)
                     /gene="bioF"
                     /locus_tag="JNZ05_002875"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HAY8566043.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10487..11527)
                     /gene="bioB"
                     /locus_tag="JNZ05_002876"
     CDS             complement(10487..11527)
                     /gene="bioB"
                     /locus_tag="JNZ05_002876"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HAY8566044.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            11614..12903
                     /gene="bioA"
                     /locus_tag="JNZ05_002877"
     CDS             11614..12903
                     /gene="bioA"
                     /locus_tag="JNZ05_002877"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HAY8566045.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12881..13351
                     /locus_tag="JNZ05_002878"
     CDS             12881..13351
                     /locus_tag="JNZ05_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HAY8566046.1"
                     /translation="MKHFFANNGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTKSF
                     VVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTGYD
                     GAAPPKGETHRYIFTVHALDVERIDVDEGGSGAMVGFNVHFHSLASASITAMFS"
     gene            13666..13749
                     /locus_tag="JNZ05_002879"
                     /pseudo
     CDS             13666..13749
                     /locus_tag="JNZ05_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958651.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            13905..14906
                     /locus_tag="JNZ05_002880"
     CDS             13905..14906
                     /locus_tag="JNZ05_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8566047.1"
                     /translation="MHKSNCFDTARLVAAMMVLVSHHYALSGQPEPYLFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSILTYFLFGWILNDFSAE
                     YFSHDIVRKTISSIFMSQAPDADITSHLIHAGINGSLWTLPLEFLCYIITGVAVALLK
                     NGKAFIVILLVFVSLSLIGSVSENRDVMFSIPLWLYPLRGLAFFFGATMAMYEKSWNV
                     SNVKITVVSLLAMYAYASYGKGIDYTMTCYILVSFSTIAICTSVGDPLVKGRFDYSYG
                     VYIYAFPVQQVVINTLHMGFYPSMLLSAVTVLFLSHLSWNLVEKRFLTRSSPKLSLD"
     gene            complement(14940..>15458)
                     /locus_tag="JNZ05_002881"
     CDS             complement(14940..>15458)
                     /locus_tag="JNZ05_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HAY8566048.1"
                     /translation="GVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTGDEWAVNIV
                     DINECVSFDKCIFNKPLCYLRSGGVSVTDCYLAGITEVQKPEGRYNTYRGCSGVPSVN
                     GIINVPVAVGATSGSAAIPNPGNLTYRVRSLFGDPASSGDKVSVSVVTINVTRPSPVG
                     VALPSMVEYLAI"
BASE COUNT         3644 a         4089 c         3884 g         3841 t
ORIGIN      
        1 aatgagccat ataccgttta atatcctggc ctcgtgctga aaatctaatt tattgctgtt
       61 ttgcgagtat gattccattc ttttagttgt ccttagaaaa aatattaata ttcaccatca
      121 ataaatttaa aaccacgcat acaaaatact tttgtattta tggatacgga aaacgattcc
      181 gctcgctcat aaggcatatt ttatacctgg tacttgttaa gtgtcgtttt tagtaagaaa
      241 aaaatcccga cacatggccg ggatagtgat ggtgaaaaag aattaacggc ggttgccgaa
      301 gatccgtaac aacatcagga acaggttgat gaagtccaga tacaaggtta acgcgccaag
      361 aatggaatat ttgcgcaggt tcgacgtgtc gcgggtatca atctgctcac ccatattttt
      421 caatttctga gtgtcatacg ccgtcaatcc gacaaagaca atcacgccga tgtaggtaac
      481 tgcccacatc aatgcttcgc ttttcagcca gaagttgacc agcgatgcca gcacaatgcc
      541 gattaacgcc ataaacagca tattgcccaa gccacttaaa tcgcgcttcg tggtgtaacc
      601 gtacaggctc attgcgccga acatcccggc agtaacgacg aaagtactgg cgatagaagc
      661 agcggtatag acaatgaata tactggaaag cgtaagaccc gtcagcgccg aataaagcat
      721 aaagagcatc gtcgttacac ctgcgctcag cttttgaatc atcgctgata acacaataac
      781 caatgctaat tgcgcgatga tcagaccgat taaaaagaca cggttagtga acaacagctc
      841 catcacggcc gcggaattag ccgcatacca ggcaacaaat gcggtcagca acaagccaac
      901 ggtcatccag ccatagactt gcgccatata agtttgcaag ccagcccggg gttgtacgat
      961 tgaatcagaa cgtgggaatc tgtccatgac gatctcctga agatataagg aatatcttaa
     1021 ggatactgca aaatgatgag gctgtgcatc gacgcagcgt aaacgcatgt actgagcggt
     1081 gaaattgccg gacgcagcgg tgccttatcc ggctaacaaa aaactaccag cgttttgccg
     1141 cctgcagatc gctctcccgg gcttcaaccc agcggtcgcc ttccggcgtg gcttcgcgct
     1201 tccagaacgg tgcgcgggtt ttgagataat ccataataaa ctgcccggct tcaaacgcac
     1261 tgctgcgatg cgcactggtg acaccgacaa aaacgatttc atcgcccggc cataattccc
     1321 cgatgcggtg aatcaccgtg acgcgcccca gcggccagcg gttacgcgct tcatcaacaa
     1381 tttctgccag tgctttttca gtcatccccg gatagtgttc gagggttaat gctttgacgc
     1441 tgtcgcccag gttatggttg cgcacctttc cggtgaaggt gactaccgca ccgtcttcgt
     1501 cacgctccgc cagccacggg tactcttctc ctacgctgaa cggctgcggg ccgacaacaa
     1561 ttttggtttc tgccatctta acctccggtt accggcggga ggaaagctac ttcgtcgccg
     1621 tcagtcagcg gatggtcaaa actcaccagc gtctggttga cggcagccag caatttgcca
     1681 tcttccagcg ccagcgccca gcgatcgctc tgcgcagcca ggtgctggcg taacgcttca
     1741 acggttgggt aatccgcagc cacttcggtt gcatctgttc ccaccaactc gcgcacctgg
     1801 gcgaaaaaaa gaactttaat catccgcttc caccttaaag tcacccgact tgccgccgct
     1861 cttcgccagc aaacgtaccg gaccaatcac catatctttt tgcaccgctt tgcacatgtc
     1921 ataaatggtc agcgccgcca cggaggccgc ggttaacgct tccatttcga caccggtttt
     1981 cccggtcagg cggcataagg tttctatacg cacccgattg tgctccggct cggcctgtaa
     2041 attgacttca actttgctga gcatcagcgg gtgacacagc gggatcagat cccaagtgcg
     2101 ttttgccgcc tgaataccgg caatacgcgc agtggcaaat acgtcgcctt tgtggtggcg
     2161 accatcaata atcatcgcca gcgtctcgct gcgcatggtg acaaaggctt ccgcccgcgc
     2221 ttcacgcacg gtttccgctt tggcggagac atccaccatg tgcgcttcgc cagcggcgtt
     2281 gatatgggtc agttgcgaca tacttatttc ttcaaatgtg gatggaaatt acacggacgc
     2341 gtacgggcat ccagctgcgg cgcgatgata ttttcccatg cggtacggca cgctttggtc
     2401 gaacccggca tggcgaaaat cagcgttttg ttagcgacgc ccgctaccgc acgagattgc
     2461 aacgtggaag tgccaatctc ttcaaacgac aacatacgga acacttcacc aaaaccttca
     2521 acctcacggt cgaacaacgg cagcagcgct tcgggagcct gatcaccttc cgtcaggcca
     2581 gtaccacccg taatcaatac cacttgtaca tcgtcgctgg cgatccacgc agatacctga
     2641 gcgcgaatag cgtagcggtt ttctttcaca atggctttat cgacaacgtg atggcccgct
     2701 tcttgcgccg aatcgcgcag atagtgaccg gaggtatcgt cttcttcacc gcgacgatta
     2761 gaaaccgtaa gaatagcaat acgggtcggg ataaattcag tgcttacctg actcatctga
     2821 tctctccttt tgacgtttta gccgccaatg tacgataagt tttgcgtaat accggtgttg
     2881 ttttgatgca ggaaatgggt ctgtttcttc tcccgcagcg ccgctgaaat acgcgcttcc
     2941 agcgcctgtt gctgggtatc gtcttccagc agatcgcgca ggttaacgcc gccttcacca
     3001 aacaggcaga gatggagttt accaatggag gaaacgcgca ggcggttgca agtggcgcag
     3061 aagtcttttt catacggcat gataaggcca atctctccgg cgtaatctgg atggcaaaag
     3121 acttgcgcgg gaccgtcgct gcgttgacgt aattggtgga tccagccgcg acgcagtagc
     3181 tcgtcacgca gaacctgacc agagatgtga tgcttacgga agagctcgct gccctcgccc
     3241 gtttccatca gttcgatgaa acgcagctgg atagggcgat gctggatcca gttcagaaag
     3301 gtgtcgagct ggtgatgatt aacatcacgc atcagcacgg tattgacttt gaccttctca
     3361 aaaccggcct caaatgcagc atcaatccct gccatgacct ggttgaattt atcctgcccg
     3421 gtaatagcgt gaaactggcg ggcgtccaaa ctgtcgacac tgacgttaat gccagtaagt
     3481 cccgcatcgc gccagttcgc cacatcgcgt tccagacggt aaccattggt ggtgaccgca
     3541 atctggcgga tagcgtcgtt ttcccgcaca gcggcgatga tatcggtaaa gtcgcggcgt
     3601 aaagacggct ctcctcctgt caggcgcact ttttccgtgc ccaggctggc gaaggcgcgc
     3661 gtaacccggc gaatttcatc gacggtaaga aagcctttat tggtgacgcc gctcggtttg
     3721 tagccatccg gcaggcagta ggtgcaacga aagttacaca catcggtaat cgacaggcgc
     3781 aagtagtaaa acttacgcgc aaatgcatca gtcagttgtg aagccatgta cacctttcca
     3841 gatacgggag gcgaagtcat ttcttccttc gccctggtgg caatctttac gagcattgtc
     3901 acggccaaag caccgtatca gttgacccag gtgcagaggc tagagtgttt agtggttaag
     3961 ccgatactag cgtgtaaatg agatttttac catccacact ttcgctatgc aatcatgtat
     4021 atagcgtcat gatcgtgcta tttcgctaca gaataaggga aaatggcggt tttactttga
     4081 tatacatcat cagggaggca tagcgtgcat cgtcctttag agatagaaga aaatagtttg
     4141 tatcctcaat attggcaggt taattgctgt ttccccgcaa tttgcgctac tgtagcgcgg
     4201 ataatctgac tccaggaaac tatatgcgca atcgtacgct ggctgatctt gatcgtgtcg
     4261 ttgctctcgg cggagggcat ggactgggac gcgttctctc atcactttcg tctttgggtt
     4321 ctcgtttaac gggtatcgtc accaccacgg ataatggtgg ctcgacgggg cgtattcgcc
     4381 gttcagaagg cggcattgct tggggcgata tgcgcaactg cctcaaccag ctgataacgg
     4441 aaccgagcgt cgcctccgcg atgtttgaat accgttttgg cggcaatggc gaactttccg
     4501 gtcataatct cggaaacttg atgttaaagg cgctggatca ccttagcgtg cggcctctgg
     4561 aagccatcaa tttaattcgt aatctgctga aagtggatac gcatttgatt ccaatgtcag
     4621 agcatcctgt tgatctgatg gcgattgacg atcaggggca tgaagtttac ggcgaggtca
     4681 atatcgacca gttaactacg ccgattcaag agttattgtt aacgcctaat gtacccgcaa
     4741 cgcgtgaggc ggttcacgct atcaatgaag cggatctcat cattattggg cctggcagtt
     4801 tttataccag cctgatgcca attctgctgc tgaaggaaat cgcccaggca ttacgccgca
     4861 cgccagcgcc gatggtttat atcggcaatc tggggcgtga gttgagttta cctgcggcta
     4921 atttgaagct ggaaagcaag ctggcaatta tggagcagta tgttggtaaa aaggtcattg
     4981 atgcggtcat cgtcgggcca aaagtggatg tctcggcggt gaaagagcgg attgtgatcc
     5041 aagaggtact ggaggccagc gatattccgt atcgtcatga ccgccagttg ttacataacg
     5101 cgctggaaaa ggcgttacag gctttaggtt aacggaaatc cgaaggaaaa ttccggcttc
     5161 ctattgaaga caaagtgcgc gttgtttatg ccggatgcgg cgtgaacgcc ttatccggcc
     5221 tacaaaccgc gcaaattcaa tatattgcgg agaaaatgta ggcctgataa gcgtagcgca
     5281 tcaggctgtt ttccgtttgt catcagtctt cttcgctatc ctgttacgat gcggcaataa
     5341 acaactcacg caactgatgc aactggtcac gaatttgtgc cgcttcttcg aactccagat
     5401 tctgcgcgtg ttgcatcatc agcccttcca gttcatggat tttctgctgc aacgctttcg
     5461 gcgacatatc catcggcaca ttatccggct caacaatcgg gcgcgatttt cctctgccct
     5521 tcgctttggt tttggcaatg ttctgcccca gcgccaggat atctaccact ttcttgttca
     5581 agccttgcgg cgtaatgccg tgttcctcgt tgtacttctg ctgtttctca cggcgacgtt
     5641 cggtttcgcc aatcgctttc gccattgatg gagtgatctt atcgccgtag agaatcgctt
     5701 taccgttaac gttacgtgcc gcacgaccaa tggtctggat caacgaacgt tcggaacgca
     5761 ggaagccttc tttgtcagcg tcgaggatcg ccaccagcga cacttccggc atatccagac
     5821 cttcacgcag taagttgatc cctaccaata cgtcgaactc acccagacgc aagtcgcgga
     5881 taatctccat acgctcgacg gtgtcgatat ctgagtgaag ataacgcacg cgctcaccgt
     5941 gttcttcgag atattcggta agatcttccg ccattcgctt ggtcagtgtg gtgaccagca
     6001 cgcgttcgtt aattgccgct cgctgacgaa tctccgaaag aagatcatca acctgtgtcg
     6061 ccaccggccg cacttcgata atcgggtcaa gcaatccggt tggacgcacc acctgatcca
     6121 ccacatcgcc gccggatttt tccagctcgt aattacccgg cgtcgccgaa acatagatgg
     6181 tttgcggcgc taatgcttcg aactcttcaa acttaagcgg acggttatcc agcgctgatg
     6241 gcaggcggaa gccgtactcc accagtgtct ctttacgcgc ccggtcaccg cgatacatgc
     6301 cgccaatttg tggaatggtg acgtgagatt catcgacgac cagcagccca tcggcaggca
     6361 ggtaatcaaa cagcgtcggc ggtggctcac ccggtccacg accggagagg aagcgcgagt
     6421 agttttcaat ccccgaacag tagcccagct cgttcatcat ctccagatca aactgggtac
     6481 gctgggtcag ccgctgctct tccagcagtt tgttgttttc caacagcact ttgcgtctgg
     6541 cggccagttc ttctttgatc tcctccatcg cctgtacgat gcgctcgcgc ggtgtgacgt
     6601 agtgcgtttt cgggtagatg gtaaaacgtg gaatagtgga aacaatctgc ccggtcagcg
     6661 ggtcaaataa cgacaatcgt tccacttcct cgtcaaacag ttccacgcga agtgcaatgt
     6721 catccgattc tgccgggaag atatctatca cctcgccacg aacgcggaaa gtaccacgct
     6781 ggaatgcttg atcattacga gcgtattgca gctccgccag tcggcgcaga atcgcgcgct
     6841 gatcgataat cataccgacc gtgagatgga gcatcatctt gagatataaa tcaggatcgc
     6901 ccagaccata aatcgcggaa acagacgcca ctacaatcac atcacgccgc tccagcatcg
     6961 ctttggtggc ggacaaacgc atctgctcaa tatgttcgtt aaccgaggca tctttctcaa
     7021 tgaaggtgtc ggaactcggt acataggctt ccggctgata gtagtcgtag taggagacga
     7081 aatactccac cgcgttttcc gggaagaact ctttcatttc gccatacagc tgggccgcca
     7141 gcgttttgtt gggcgcaagt accatggttg ggcgctgaag gtcagcaatg acattggcaa
     7201 tggtgaaggt tttcccggag ccagtcacgc caagtaacgt ctggtgcgcc aggccatctt
     7261 ccagcccctc ttcgagacgt cgaatcgcct ctggctgatc gccagaaggt ttaaaagcgg
     7321 aattcagttt gaacggttta ctcatgagtc gctacctgaa ggagttgggc gggcaggtat
     7381 gtaattttac tcgtcgtact taattatgcc aacaaattat actggataaa aaaacagttc
     7441 atcaccataa tatttctgat acagcgtaaa ctccgctgtc aatcatgagc aaaatttact
     7501 ctgtggcgag ataaaactcc ggccttaccg ggttatcccc aaagcaacgg cttttttaac
     7561 atttgtcaag atgagtgatg acagttttat ggcaagagat gcctgtacag tgactgccat
     7621 tgcatttatc taaccagtta aaaaataaaa gatatttctt tgagccgtct ttaacgccaa
     7681 ttcgcgtgca agccgcgtat tctctcgctt gcctcgtgtt ttctaactct aatacacatg
     7741 gttatccaca ggaatagtgg ataactgtct ccagccccta tcccccgccg cctgggcaat
     7801 tcccacattc gccgcatgag gcggaagtaa aatttttttc gtgattattt tagaatttaa
     7861 ttggttaaat tgcagtcaat cgaagacgcg atctcgctcg caatttaacc aaatacagaa
     7921 tggctacaac aaggcaaggt ttatgtactt tccggttgcc gcattttctg gattttctgc
     7981 aagccagggg atctctccca gcagcggcgc gggaatcatg cgggtgagcg tggtcatata
     8041 ttcagcgtga cgttttcccg gaggcgtaac atcgttcgcc acccaacccg ccagagtcag
     8101 tccggcgtgt tgtattacct gcgcagtcaa catcgcgtga ttaatacagc cgagtttcac
     8161 accaactacc agtatcaccg gcagttgttc ctgtgttacc caatctgcaa aagtgaaagt
     8221 gtcagaaagc ggcgtaaacc agccgccagc accttccact aacacccagt cagcctgttg
     8281 ttcaagcgcg cgtaatccgg cgctcattac caatgattct atcggcctgc cctcttgggc
     8341 gctgatgatg tgcggcgagg agggttctgc gaaggtgtaa ggatttaccg ttgcgtaatc
     8401 cagctgcagg ctgctgttgt gctgtaacgc cagcgcgtca ctattacgta agccttccgg
     8461 ggtcttttcg ctgccagagg cgaccggttt ataacctgcc gtccgataac ctactgcctt
     8521 cgcggcttgt aaaagtgcac aactggcgac ggttttcccc acttcggtat ccgttccggt
     8581 gacaaaataa cgtttactca cgagcaatca ctcccaaaaa aagatgatac gtcagaggat
     8641 atcgcccctg ttgttgcggc caggccagtt gcaatcgctg caactgcgaa cgcgttaata
     8701 ttcgcgggtc gcgcccttca tgaagatgcg tagcaccgat gcctttcagc gaacgcatgg
     8761 cactgagcgc atcatcaaac catagtgtga tgagctgaat atgatgttga taacgcacgc
     8821 cgttcagcga ctcttcgatt tcatctggcg gtaaaaagcg attagcatgc ggacgctcgt
     8881 ccaccgcctg ccgcgcctga tgcagttcgg gtaacgatcc ctgcaccagc gtggtaaacg
     8941 cgaccacgcc tccggggcgc accacccgat acagctcgcg gagtgccgtg gataaattac
     9001 cgcaccactg caccgcgaga ttgctccatg caagatcgaa cgtcgcagtc gctaacggca
     9061 gggattcgat atctcccgcc agataatgat ctgcggcatc tttatggcgg gcttgcgcaa
     9121 gcattggcgg cgagagatct aacgccgtca cctgcgcgtg acgttcccgc cagtggcggc
     9181 tcatccagcc aggtccacaa cccgcgtcca gtacgtgggt gtatttacgc tgtggaagca
     9241 ttgccagtaa ggcgtcagca ctctggcgct gtagatctgc atgttgctca tagtgtgcgg
     9301 ctgcccgacc aaatgccgct gcaatggctt gtttattaac cgttgccatg cagcacctcc
     9361 agcagacgat cgatatcctg catttcatgc gcagcggtta gcgttaagcg cagtcgcgca
     9421 gtaccagcgg gtacggttgg cgggcgaatc gccgtgaccc agcagccttg ctggcgcagt
     9481 ttttctgcca gttgtaacgc acggctgtta tcaccgacga tcaatggctg gatggcgctg
     9541 catgaatcag ccagcgtaaa cggcaaatcc tgtactccgg cacgaaaacg cgcaatgagt
     9601 gacaccagtt tttcgcgccg tgcatccccc tcatcacggc gaatgaccgc cagcgacgca
     9661 cgtaatgcct gcgcctgagc gggcggcata ctggtgctgt agataaggtg gcgggcgaat
     9721 tgcagcagat aatccgccac cgtacttgag caaagcaccg cagccccact gacgccaaat
     9781 cctttgccaa aagtcactac cagcaattct ggttttacct tttgcagcca gcagctgccg
     9841 cgcccctgct ccccgataac gcccgtgccg tgggcatcat cgaccatcaa ccagccattg
     9901 tgctgttgcg ttacctgctg gatttccgcc agtggcgcgc tatcgccgtc catgctgaac
     9961 acgccttctg tcaccaccag ttgctgcccc ggacaggggg aagcaagcag tcgcgccaga
    10021 tgagcgacat cgttatgagt aaaacggcga agctgcgacg ggcttaaact ggcagcctcc
    10081 agcaatgagg catggctaag ccggtcggca acaatacggt cctctttcgc catcatcgcg
    10141 gcaatgactg cctgattcgc ggcaaaacca gagataaaca gcagcgcccg cgaatagcca
    10201 agccactcgg ccagttcttc ttccagcgcc tgatgcacca cgctataacc gctgacgtga
    10261 ccggagccgc cgctaccgat gccaaattgc tccgccccct gcttccaggc acggataatt
    10321 tgcggatgat ggcttaaacc taaataatcg ttactggaaa agttcagata ctggcgatca
    10381 tccgccacca gccagcgtcc ggctccttgc gccaccggat aacggcgacg cagggcatcg
    10441 gcagcacgcc gcgcatcgag cgccgcgttg attttctcct gccagctcat aatgctgccg
    10501 cgttgtaata ttcgtcggtg tccggggtca tcagcgcctg tccaagacgt tgctgttgtt
    10561 cgttatcccc tgccagcacg gcagtttgct gcggatttag ccccagtttg cggaacagtt
    10621 gcaggtcttt atcttcttcc ggattcggcg tggtcagcag tttgcaaccg tagaaaatcg
    10681 agtttgcgcc tgccataaag cacatcgcct gagtctgttc gttcatctgc tcgcgtccgg
    10741 cagaaaggcg cacgtaagag gtcggcatca tgatccgcgc tacggcaatg gtgcgaataa
    10801 aatcaaaggc atcaacgtca tcgttatcgg caagcggcgt gcctttcacc ttcaccagca
    10861 tgttgattgg tacgctttcc ggcggcgtcg gcaggtttgc cagttgcagc aataatccgg
    10921 cgcgatcttt taccgtttcg cctaagccca caatgccacc tgagcagact ttgatcccgg
    10981 catcgcgcac tttttccagc gtatcgaggc gttcctgata agtgcgtgtg gtgatgatat
    11041 tgccgtaaaa ctccggcgag gtgtccaggt tgtggttgta gtaatccagc ccggcgttcg
    11101 cgaggcgctg cgcctgagat tcactcaacg tgcccagcgt catacacgcc tccagcccca
    11161 tcgcttttac cccctgcacc atttgttcca ggtacggcat atcgcgttcg tggggattct
    11221 tccatgccgc gcccatacag aagcgcgtcg atcctgccgc tttcgctttg cgcgccgact
    11281 ccagcacctg ttcaacttcc atcaaccgct cggcttccag cccggttttg tagcgcgagc
    11341 tttgcgggca gtatttgcaa tcttccggac aagctccggt cttaatcgac agcaacgtgc
    11401 tgacctgcac ctgacgagga tcgaaatgct ggcgatgcac ctgctgcgct tcaaacagca
    11461 gatccagcaa cggtttttca aataattctg tgacttgcga caatgtccag cgtgggcggt
    11521 gagccatggg gcttctccaa aacgtgtttt ttgttgttaa ttcggtgtag gcttgtaaac
    11581 ctaaatcttt tcaatttggt ttacaagtcg attatgacaa cggacgatct tgcctttgac
    11641 caacgccata tctggcaccc atacacatcc atgacctccc ctctgccggt ttatccggtg
    11701 gcgagcgccg aaggttgcga gctaattttg tctgacggca gacgcctggt tgacggtatg
    11761 tcgtcctggt gggcggcgat ccacggctac aatcacccgc agcttaatgc ggcgatgaag
    11821 acgcaaattg atgccatgtc gcatgtgatg tttggcggta tcacccatgc gcccgccatc
    11881 gagctatgcc gcaaactggt ggcaatgacg ccgcaaccgc tggagtgcgt ttttctcgcg
    11941 gactccggtt ccgtagcggt ggaagtggcg atgaaaatgg cgttgcagta ctggcaagcc
    12001 aaaggcgaag cgcgccagcg ttttctgacc ttccgcaatg gttatcatgg cgataccttt
    12061 ggcgcgatgt cggtgtgcga tccggacaac tcaatgcaca gcctgtggaa aggctatctg
    12121 ccagaaaact tgtttgctcc cgccccgcaa agccgcatgg atggtgaatg ggatgaacgc
    12181 gatatggttg gttttgcccg cctgatggcg gcgcatcgtc atgaaatcgc agcggtgatc
    12241 attgagccga ttgtccaggg cgcaggcggg atgcgcatgt accatccgga atggttaaaa
    12301 cgaatccgca aaatgtgcga tcgcgaaggt atcttgctga ttgccgacga gatcgccacc
    12361 ggatttggtc gtaccggcaa actgtttgcc tgtgaatatg cagaaatcgc gccggacatt
    12421 ttgtgcctcg gtaaagcctt aaccggcggc acaatgaccc tttccgccac acttaccacg
    12481 cgcgaggttg cagaaaccat cagtaacggc gaagccggct gctttatgca tgggccaact
    12541 tttatgggca atccgctggc ctgcgcggca gcaaacgcca gcctggcgat tatcgaatcc
    12601 ggcgaatggc agcagcaggt ggcggctatt gaagtgcagc tgcgcgagca actggcacca
    12661 gcccgtgatg ccgaaatggt tgccgatgtg cgcgtactgg gggcaatcgg tgtggtcgaa
    12721 accactcgtc cggtgaatat ggcggcgctg caaaaattct ttgtcgaaca gggggtatgg
    12781 atccggcctt ttggcaaact gatttacctg atgccgccct atattattct cccgcaacag
    12841 ttgcagcgtc tgaccgcagc ggttaaccgc gcggtacagg atgaaacatt tttttgccaa
    12901 taatggcgat aagttgccgc atcgtcatgt ctttaacggc atgggttacg atggcgataa
    12961 tatttcaccg catctggcgt gggatgatgt tcctgcggga acgaaaagtt ttgttgtcac
    13021 ctgctacgac ccggatgcgc caaccggctc cggctggtgg cactgggtag ttgttaattt
    13081 acccgctgat acccgcgtat taccgcaagg gtttggctct ggtctggtag caatgccaga
    13141 cggcgttttg cagacgcgta ccgattttgg taaaaccgga tacgatggcg cagcgccgcc
    13201 gaaaggcgaa acccatcgct acatttttac cgttcacgcg ctggatgtag agcgtattga
    13261 tgtcgatgaa ggaggcagcg gcgcgatggt cgggtttaac gttcatttcc actctctggc
    13321 aagcgcatcg attaccgcga tgtttagtta atcactctgc cagatggcgc aatgccatct
    13381 ggtatcactt aaaggtatta aaaacaactt tttgtctttt taccttcccg tttcgctcaa
    13441 gttagtataa aaaagctgaa cgagaaacat aaaacgctgt aaatatcaat atgttatgaa
    13501 agatttagtc taaataatag actgcataat actacaaaac acaacatatc cagtcactat
    13561 gaatcaacta cttagatagt attagtgacc tgaggcagag cattagcgca aggtgatttt
    13621 ttgtcctctt gcgctaattt tttgtcaacg gactgggtta gttgaattta caaccatgct
    13681 aagtatctcg ataagcgcag agaaatgatg cagtggtggg cggattggct tgatgagaag
    13741 gttaggtagg caaatccatg cctgagcaga gccttgccct ctacctgagc ccatgctcat
    13801 tctttggcct caacgcgagc atgacctcac tggctggacc caaaatggac tatgacagaa
    13861 agatctgata tttttcctcg caaaaactta gaggctttat tttaatgcat aagagcaact
    13921 gctttgacac ggcaaggctt gtggcagcta tgatggttct ggtcagtcac cactacgcgc
    13981 tctctgggca gccggagccg tacctgttcg gctttgaaag tgctggcggc attgcagtta
    14041 tcatattctt ttcaatatca ggatatctga tttcaaaatc agctatcagg agtgattcct
    14101 ttattgattt catggctaaa agagcgagaa gaatattccc ggcgctggtc ccttgctcta
    14161 tactgacata ttttttattt ggatggatac ttaacgactt cagcgcagaa tattttagcc
    14221 atgacattgt caggaagacc ataagctcta tttttatgag tcaggctcca gatgctgaca
    14281 ttacatcaca tttaattcat gccggaatta acggcagttt gtggacccta ccgcttgagt
    14341 tcctgtgtta tataataacg ggagtagcag ttgccctact aaagaacgga aaagcattca
    14401 tagttatact gttggttttt gtttctcttt cattaattgg aagtgtgtca gaaaacaggg
    14461 atgtaatgtt ctctattccg ttatggctgt atcctttgcg tggactggcg ttcttttttg
    14521 gtgccactat ggcaatgtat gagaagtcat ggaatgtaag taatgttaaa attactgtag
    14581 tttctttgct tgctatgtac gcctacgcta gttatggcaa aggcattgat tatactatga
    14641 cttgctatat cctggtttca ttctcgacaa tagccatatg cacatccgtt ggcgacccac
    14701 tggttaaggg caggtttgat tactcgtatg gtgtgtacat ttatgcattc ccggttcagc
    14761 aggttgttat aaacacattg cacatgggtt tttacccgtc aatgttgtta agtgccgtga
    14821 ctgttctttt cttgtcccat ctttcatgga atctggttga aaaaagattc ctgacacgaa
    14881 gttcgcctaa attatccctg gattgaacaa taaaaggggc catatggccc ctaaaataat
    14941 cagatggcca gatactcaac cattgaaggc agcgcgactc ctaccgggct tgggcgagta
    15001 acattaattg tcaccacgga aacactaacc ttgtcaccac ttgatgcagg gtcaccaaaa
    15061 aggcttctta ctctgtatgt caggttcccc gggttcggta tagctgcaga tccgcttgtg
    15121 gcacctaccg caactgggac attaatgatc ccatttacag acggtacgcc tgaacagcca
    15181 cgatacgtat tgtatctgcc ttccggtttc tgcacctcgg taatgccagc caaataacag
    15241 tcagtaactg acacgccacc gctacgaaga tagcatagag gcttattgaa tatgcactta
    15301 tcgaaagaaa cacattcatt gatgtcgaca atatttacag cccattcatc accagttaac
    15361 gtacaaccac ttacaaagac gaagatcgcc gactgttcta tcgctatact ttgattgttt
    15421 gcagccgtgt ttttgtttgc agtgttgata tgaacacc
//
LOCUS       DADMEQ010000091        15021 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.092, whole genome shotgun sequence.
ACCESSION   DADMEQ010000091 DADMEQ010000000
VERSION     DADMEQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15021
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.092"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..236
                     /locus_tag="JNZ05_002882"
     CDS             <1..236
                     /locus_tag="JNZ05_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HAY8566049.1"
                     /translation="LGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPA
                     LSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            246..884
                     /locus_tag="JNZ05_002883"
     CDS             246..884
                     /locus_tag="JNZ05_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HAY8566050.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            887..2050
                     /locus_tag="JNZ05_002884"
     CDS             887..2050
                     /locus_tag="JNZ05_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY8566051.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2134..3758
                     /locus_tag="JNZ05_002885"
                     /pseudo
     CDS             2134..3758
                     /locus_tag="JNZ05_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
     gene            complement(3875..4150)
                     /gene="yjfN"
                     /locus_tag="JNZ05_002886"
     CDS             complement(3875..4150)
                     /gene="yjfN"
                     /locus_tag="JNZ05_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HAY8566052.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4299..4628)
                     /gene="bsmA"
                     /locus_tag="JNZ05_002887"
     CDS             complement(4299..4628)
                     /gene="bsmA"
                     /locus_tag="JNZ05_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HAY8566053.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4810..5559
                     /gene="yjfP"
                     /locus_tag="JNZ05_002888"
     CDS             4810..5559
                     /gene="yjfP"
                     /locus_tag="JNZ05_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY8566054.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5556..6311)
                     /gene="ulaR"
                     /locus_tag="JNZ05_002889"
     CDS             complement(5556..6311)
                     /gene="ulaR"
                     /locus_tag="JNZ05_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HAY8566055.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6419..7483)
                     /gene="ulaG"
                     /locus_tag="JNZ05_002890"
     CDS             complement(6419..7483)
                     /gene="ulaG"
                     /locus_tag="JNZ05_002890"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAY8566056.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCANDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7838..9235
                     /gene="ulaA"
                     /locus_tag="JNZ05_002891"
     CDS             7838..9235
                     /gene="ulaA"
                     /locus_tag="JNZ05_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAY8566057.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9251..9556
                     /gene="ulaB"
                     /locus_tag="JNZ05_002892"
     CDS             9251..9556
                     /gene="ulaB"
                     /locus_tag="JNZ05_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HAY8566058.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9566..10030
                     /gene="ulaC"
                     /locus_tag="JNZ05_002893"
     CDS             9566..10030
                     /gene="ulaC"
                     /locus_tag="JNZ05_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HAY8566059.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAVVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10044..10689
                     /gene="ulaD"
                     /locus_tag="JNZ05_002894"
                     /pseudo
     CDS             10044..10689
                     /gene="ulaD"
                     /locus_tag="JNZ05_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
     gene            10699..11534
                     /locus_tag="JNZ05_002895"
                     /pseudo
     CDS             10699..11534
                     /locus_tag="JNZ05_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
     gene            11534..12220
                     /locus_tag="JNZ05_002896"
     CDS             11534..12220
                     /locus_tag="JNZ05_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY8566060.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTL
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12349..12624)
                     /locus_tag="JNZ05_002897"
     CDS             complement(12349..12624)
                     /locus_tag="JNZ05_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY8566061.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12951..13346
                     /gene="rpsF"
                     /locus_tag="JNZ05_002898"
     CDS             12951..13346
                     /gene="rpsF"
                     /locus_tag="JNZ05_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAY8566062.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNIEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13353..13667
                     /gene="priB"
                     /locus_tag="JNZ05_002899"
     CDS             13353..13667
                     /gene="priB"
                     /locus_tag="JNZ05_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HAY8566063.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13672..13899
                     /gene="rpsR"
                     /locus_tag="JNZ05_002900"
     CDS             13672..13899
                     /gene="rpsR"
                     /locus_tag="JNZ05_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAY8566064.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13941..14390
                     /gene="rplI"
                     /locus_tag="JNZ05_002901"
     CDS             13941..14390
                     /gene="rplI"
                     /locus_tag="JNZ05_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAY8566065.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14461..15018)
                     /locus_tag="JNZ05_002902"
     CDS             complement(14461..15018)
                     /locus_tag="JNZ05_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY8566066.1"
                     /translation="MNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDVE
                     SFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQLR
                     TVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRSD
                     RMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3509 a         3660 c         4047 g         3805 t
ORIGIN      
        1 tgttaggtta cgttatccca ttatccagtg cggcaatcaa tgcggtgagt attccagact
       61 atttcgcctg gggcgggatt gcactggtga ttcagttact cgtttttgct ggtgtcagac
      121 tttatatgcc tgcattaagc gaaaaaatta ttaatcacaa taccgcagca ggaatgttta
      181 tgggaaccgc cgcgctggct ggcggtattt ttaacgcagc ttgtatgaca tggtaatgga
      241 cgatcatggc cagaaaacgc aaatcaagaa ataacagtaa aattggtcac ggagcgatca
      301 gtcgcattgg tagaccgaat aatccttttg aaccgcgtcg caatcgctac gcacaaaaat
      361 acttaacgtt ggcgctaatg ggcggtgccg cttttttcgt attgaaaggt tgtagcgata
      421 gtagcgatgt cgataacgac ggcgacggaa cgttttacgc gacagtgcag gattgtattg
      481 atgacggtaa taatgccgac atttgcgcgc gtggctggaa caacgccaaa gcggcatttt
      541 atgccgatgt tccaaagaat atgactcagc agaactgtca gtctaagtac gaaaattgct
      601 actacgacaa tgttgaacag agttggatcc cggtcgtttc tggatttttg ttaagccggg
      661 ttattcgtaa agatcgcgat gagccgttcg tctataacag cggcggatcc tcatttgctt
      721 cccgccccgt ctggcgcaac acttccggtg attactcctg gcgcttcggt tctggcaaaa
      781 aagagtctta ctcttcaggc ggctttacca ccagaaaagc gtcgaccgtt tcgcgcggcg
      841 gctatggtcg ttcttccagc gcccgtgggc attggggagg ctaatcatgc tgagacacaa
      901 cgttcctgtg cggcgggatc tggaccagat cgccgctaac aacggtttcg actttcatat
      961 catcgacaat gaaatctatt gggacgagag tcgggcttac cgttttactc tgcgccagat
     1021 tgaagagcag atcgaaaaac cgactgcgga actgcatcag atgtgccttg aggtggtgga
     1081 tcgcgcggtt aaagacgaag agatcctgac gcaactggcg atcccgccgt tgtactggga
     1141 tgtgatcgcc gaaagctggc gcgcacgcga tccttcgctg tatggccgga tggattttgc
     1201 ctggtgtggc aatgcgccgg tgaagctgct ggagtacaac gccgatacgc cgacctcgtt
     1261 gtatgagtcg gcttatttcc agtggttgtg gctggaggat gcccggcgca gcggcattat
     1321 tccgcgtgat gccgatcagt acaacgccat tcaggaacgc ctgatttcgc gctttagtga
     1381 gctttacagt cgggaaccgt tttatttttg ctgctgtcag gacaccgatg aagacaggag
     1441 taccgtgctg tacttgcagg actgcgccca gcaggcaggg caggagtcgc ggtttatcta
     1501 cattgaagat ctcggtttgg gcgtcggcgg cgtactgacc gatcttgatg ataatgtcat
     1561 ccagcgtgca tttaagctgt atccgctgga gtggatgatg cgtgacgata acggtccgct
     1621 gctgcgcaag cgtcgcgagc aatgggtgga gccgttatgg aaaagtatct tgagtaataa
     1681 agggctaatg ccgctgcttt ggcgcttctt ccctggtcat cctaaccttc ttgcatcctg
     1741 gtttgagggt gaaaaatcgc agattgccgc tggcgaaagc tatgtgcgta aaccgatcta
     1801 ctcgcgcgaa ggcggaaacg tcaccatttt cgacggtcag aataacgtcg ttgaccacgc
     1861 tgatggtgat tacgccgatg aaccgatgat ctaccaggcg tttcaacctc tgccgcgatt
     1921 tggcgatagc tacacactca tcggtagctg gattgtcgat gatgaagcgt gcggaatggg
     1981 gatccgtgaa gataacacac tgatcaccaa agacacctca cgtttcgttc cgcattacat
     2041 tgctggataa gaatgtttta gcaatctctt tctgtcatga atccatggca gtgaccatac
     2101 taatggtgac tgccattgat ggagggagac acagtgcact ggcaaactca caccattttt
     2161 aatcaaccta taccattaaa taacagcaac ttatacctgt ctgatggcgc gctttgcgaa
     2221 gcggtaacgc gtgaaggtgc tggctgggat agcgattttc tagccagtat tggtcagcag
     2281 ttaggaacgg ctgaatccct tgaactgggg cggctggcga atgtaaaccc gcctgaactg
     2341 ttgcgctacg atgcccaggg gcggcgtctg gacgatgttc gtttccatcc cgcatggcac
     2401 ctgctgatgc aggcgttatg taccaatcgg gtgcacaatc ttgcctggga agaagacgct
     2461 cgctccggcg catttgtggc gcgcgcggcg cgttttatgt tacatggcag gttgaggctg
     2521 ggtcgttatg tccaataacg atgacctttg ccgccacgcc attgttgtta cagatgttac
     2581 ccacgccgtt tcaggactgg accacgccgc tgctgagcga tcgctacgat tctcacttat
     2641 tgccaggtgg gcaaaaacgc ggtttgttga ttggcatggg aatgacggaa aagcagggcg
     2701 gttccgatgt catgagcaac accatccgtg cagagcgttt ggaagatggc tcttatcggc
     2761 tggtggggca taaatggttt ttctcggtgc cgcaaagcga tgcgcatctg gtgctggcgc
     2821 agaccgcggg cggtctgtcc tgcttttttg tgccgcgctt tttgcctgac gggcaacgca
     2881 acgcgattcg cctcgagcgg ctgaaagata agctgggtaa tcgctctaac gccagttgcg
     2941 aagtggagtt tcaggatgcc attggctggt tattggggca ggaaggggaa ggaattcgtc
     3001 tgatcctgaa aatggggggg atgacgcgtt ttgattgcgc cctgggtagc catgccatga
     3061 tgcgccgtgc attttcgctg gcgatttatc atgcacatca acgccatgtt tttggtaatc
     3121 cattgatcca acagcccctt atgcgtcatg tcttaagtcg catggcactt cagcttgaag
     3181 ggcaaacggc gttgctgttt cgtcttgcgc gagcgtggga ccggcgtgcc gatgccaaag
     3241 aagccctgtg ggcgcgttta tttacgcctg cggcaaaatt tgtgatctgc aaacgaggta
     3301 tgccgtttgt ggccgaagcg atggaggtgc tgggcggcat tggttattgc gaggagagcg
     3361 agctgccgcg gctttaccgg gagatgccgg taaacagtat ttgggaaggt tccggcaata
     3421 ttatgtgcct ggatgtgttg cgcgttctca ataagcaagc gggcgtatac gacttattgt
     3481 cggaagcatt tgtggaagtg aaagggcagg atcgctattt tgatcgcgcg gttcgtcgtt
     3541 tacagcagca gctgcgtaag ccagctgaag aactggggcg agagattact catcagctat
     3601 tcctgctggg ctgcggtgcg caaatgttga aatatgcttc tccgccaatg gcgcaggcgt
     3661 ggtgtcaggt gatgttagat acgcgcggcg gcgtacggtt gtcagagcag atccagaatg
     3721 atttattgct gcgggcgacg gggggagtgt gtgtgtaagc gtatacgact gatgcgacgc
     3781 tggtttcgat taactaaatg aaatatatga aaattgtagg ccggacaagg cgctcgcgcc
     3841 gcatccggca ttgttcattt gctgtagatg acactcatgc atacagtatc gcctgtacgc
     3901 gccagttatc aggctgctgg tcttcgtaca tggtaataat gcggtaccat gacgcacctt
     3961 gttcatcggc ctttaacgcc acgatacgtt ccacgtcctg cggatcccct gaaatattgc
     4021 taactgagat ctgacctatt tcattcaggc ccgtcacgca gtcagcactg gcgaattctg
     4081 cagactgtgc cgtggcgctc gcaggataag tcactaattg cagcgagggt gaggcaagta
     4141 attgtttcat cgtcagctcc atttttcgtt tccccacatg ttgcagggca ttccatcgcg
     4201 gtaataaacg cccaagcttc cagccggtaa tttattgtgc acaggtaaac gcatagggac
     4261 gcaaagcaat gtaaagctga ctatgtaagt tgtctgattt atttacggta caaaatggct
     4321 tgtgaatacc actgtcctgt cacgatggtt tcgtccacca tgacgacaac gtaataatca
     4381 gcttttgcag cgacagcttt tgctctgatt tctgctaatg cgtcatccgg agaaccccga
     4441 accatcgtgc ttacgctacc tattcgctgt aacccttgcg tctggtcgcg gcgaatctct
     4501 tgcggatggt ccgttactgg cggtgctggc tgtggcgtac cttgcagtgc gctacaggca
     4561 cttaacatca acaccaataa taaactggca aaccggtaaa taacgctatt acgtttcctg
     4621 ctaaccatag tgtagtgcct tattaattta actttggggg aatgttcccg aattgttacc
     4681 tggtacgcga ttttcgaatg aaaacgtgac gaaagcgtaa cccacatctc aacattatga
     4741 actaataccg ccagcagaaa cttgtgctaa ggatttcttc atgtcatacc agacaaaaag
     4801 gagagacaga tgattgaaat agaatcacgc gagctggcag atattcccgt tcttcatgct
     4861 tatcctgtcg ggcaaaaaga taccccgtta ccgtgcgtaa ttttttatca cggctttact
     4921 tcatccagcc tggtgtatag ctattttgcc gttgcgctgg cgcaggctgg tttgcgggtg
     4981 atcatgccgg atgcgcccga tcacggtagc cgttttagtg gcgacgaagc gcggcggtta
     5041 aatcaattct ggcaaatctt gctacaaagt atgcaggaat tcactacttt acgtgcggca
     5101 atagccgaag aaaactggct gcttgatgac cgtctggcag tcggtggcgc gtcgatgggc
     5161 gcgatgacgg cactgggaat taccgctcgc caccccacgg tgaaatgtac cgccagcatg
     5221 atgggatcgg gctattttac atcgctcgcc cgttcactgt ttccaccgct gatacctgaa
     5281 acggcagcac agcagaatga attcaataaa attgtcgcgc cgctggcaga gtgggaagcg
     5341 acaaaccacc tggaacaact tggggacaga cctctactgc tgtggcatgg cctcgacgat
     5401 gacgttgtgc ctgccgacga atcactacgt ttgcagcagg ccttaagcga gacgggacgg
     5461 gataaactgc taacctgttc atggcagcca ggcgtgcgtc accgcattac gcctgaggcg
     5521 ttagatgctg ccgtgacatt tttccgccag catctttaaa cacgcagaat gctgacccct
     5581 tgcgcttcca gttgttgcag gatttccggg ttagcatttt tgccggtgat cagcatatcg
     5641 atttgatcgg cacggctaaa aagcattccc gcgcgttcgc caatcttact gctatcaacc
     5701 agtaccacca gtttccctac cacgctcagc atcttctgct ctgccattgc tgtcagcata
     5761 tcggttttat acaacccttc tgcggtcagc ccttttccgc tggtaaacat ccagtgcccg
     5821 gcatagagac tgttttcgct gccctgcggg cttaaagtga tggactgact tttgttgtac
     5881 tgtccaccca taatgatcac gctgtcatgt tcttgatcga tcaggtaatt tgccagcggt
     5941 agataattag tgatgatttg cactggcttg ccacacattt cccgcccaag cagaaacgcg
     6001 gtggagccgc agttgatgac tacgctttcg cccggattaa ccagctgcga ggccgcttta
     6061 gcaatacgta ctttttcatc gtgattctgc gcctgatgca gattcatcgg cgtccagcgc
     6121 gggcgctgtt gggtaatagc ttctgcgcca ttgcgcacct ttttcagttt gccgctttcg
     6181 ccaagtttat tgatatcgcg tcgcgcagtg gcaggcgaaa ttcccagacg ctcaacgact
     6241 ttctcaacgg tcacaaagcc caattgtgcg agcatttcca ggaggatttg atgtctttgt
     6301 gcttcagtca tgagctattc cgataaaatt tgattttttt agatgatatt tgaaatagcc
     6361 aggaaatact acgccggata gcgcgaactt ctccacgcta tccggtataa acgacagatt
     6421 acaggaatga cttgaacggc agatccggtt caatagtgaa gcaatcatcg aaaccgcgcg
     6481 gatagtggta ctcgaagttg tctttatcca gcggccaggt aaatttgccg ccaacctgcc
     6541 agataaacgg cttgaagcca tacttcagac gatctttttt catctcccac agcacgcgga
     6601 tctcttgcgg atcggcctgg aagtttgacc agatgtcgtg gtggaacggg ataaccactt
     6661 tcgcattcag ggcttcaccc atacgcagca tatcggcgct ggtcattttg tcggtaatac
     6721 cgcgcgggtt ctcaccatac gatcctaacg ccacatcgat ctgatgttcg ttaccgtgct
     6781 tcgcataata gttagagtag tgggaatcgc cactgtgata tagggaaccg ccaggggttt
     6841 tgaacaggta gtttactgcg cgatcgtcca tgccatctgg cagtacgcca gccgcttttt
     6901 gatcggcagg cagggtgatc agtgcagtac ggtcgaaagc gtcaagcgca tgaatttcaa
     6961 tgtctttcac ttttacaaca tcgcccggtt tgaccacgat gcaacgctct ttcggtacgc
     7021 cccagccaat ccacaaatca acgcaggttt tcggtccaat gaatggtacg tcattagcac
     7081 agttctgcat cacggcagca gcgacgttaa cgtcgatatg atcgttgtga tcgtgagtcg
     7141 ccagtaccgc gtcgatctgg cgaatcgcaa acggatcaag aacaaacggg gtggtgcgta
     7201 ggtttggctg cagttttttc acgccagcca tgcgctgcat ctggtgaccc tgtttcatta
     7261 acgggttacc gtgactttgt ttgccagtgc cgcaccagaa atcaacgcaa acgttggtgc
     7321 caccttctga tttcaaccag atcccggtgc agcctagcca ccacatcgca aatgtgccag
     7381 gggcgacctg ctcttgttca atttcttcat tcagccagct accccactcc gggaaagtgc
     7441 tcaggatcca ggattctcgg gtgatacttt tcactttact catcgccatt taccttcatg
     7501 atagttcaat tcgaatcaat atgtgattgg ttttgattaa tcctgacact attttttcag
     7561 gaaggcaatg accatttttt gactttttgc cagggaagtt gttgttgatt ttgagtgtag
     7621 aaagatttaa tggaatgtat agataaatta actaaatgaa tttaaataaa taattttttc
     7681 gttgtgtgaa tcccactcta tccgtgtgga attatttgcg ggtcgcgcca catttaatca
     7741 taaataatct tgttgtgatt acttttgaaa attagagtgg gtgcacaaca ttccgggtgt
     7801 gcggaatacc cggttacctc ttcttcagga gatcgttatg gagatcctct acaacatctt
     7861 taccgtgttt tttaaccagg tcatgaccaa tgccccgttg ttgctgggta ttgtgacctg
     7921 tctgggctac atcctactgc gcaaaagtgt cagcgttatt attaaaggca cgattaaaac
     7981 cataattggt ttcatgttgt tgcaggcagg gtccggcatc ctcaccagca ccttcaaacc
     8041 ggtggtggcg aaaatgtccg aagtctacgg cattaacggc gcaatttccg atacttacgc
     8101 gtcgatgatg gcaaccatcg accgcatggg cgatgcctat agctgggtag gttacgcggt
     8161 gttgttagcg ctggcgctga acatctgtta cgtgctgctg cgtcgcatta ccggtattcg
     8221 taccatcatg ctgactggtc atatcatgtt ccagcaggcg gggctgattg ccgttacgtt
     8281 gtttattttc ggctactcca tgtggacaac cattatctgc acggcgattc tggtttcgct
     8341 ctactggggc attacctcca acatgatgta caagccgact caggaagtga cggacggctg
     8401 cggtttctcc atcggtcacc agcaacagtt tgcatcatta attgcctata aagttgcgcc
     8461 gttcctcggc aaaaaagagg aaagcgttga agacctcaaa ctgccaggct ggctgaatat
     8521 tttccacgac aacatcgtct ccacggcgat tgtcatgacc atcttctttg gtgccattct
     8581 gctctccttc ggtatcgaca ccgtgcaggc gatggcaggc aaagtgcact ggacggtcta
     8641 tatcctgcaa accggttttt ccttcgcggt ggcgatcttc atcatcactc agggtgtgcg
     8701 catgtttgtg gcggaactct ctgaagcgtt taacggtatc tcccagcgcc tgatccctgg
     8761 tgcggttctg gcgattgact gtgcggctat ttatagcttc gctccgaacg ccgtggtctg
     8821 gggctttatg tggggcacca tcggtcagct gattgcggtt ggcatcctgg tcgcctgcgg
     8881 ttcctcgatc ctgattatcc caggctttat cccgatgttc ttctccaacg ccaccatcgg
     8941 cgtgttcgct aaccacttcg gcggctggcg tgcggcgctg aagatttgtc tggtgatggg
     9001 gatgatcgaa atctttggtt gcgtctgggt ggtgaaactt accggtatga gtgcctggat
     9061 ggggatggcg gactggtcga ttctggcacc gccgatgatg cagggcttct tctccatcgg
     9121 tatcgccttt atggccgtca tcattgtaat tgcactggct tatatgttct tcgctggccg
     9181 cgcactgcgc gcagaagaag atgcagaaaa acaattggca gaacagtctg cttaataagg
     9241 agttttgatt atgaccgtac gtattctggc tgtgtgtggc aacggacaag gcagttccat
     9301 gatcatgaag atgaaagtgg accagttttt aacccaatca aacattgacc acacggtaaa
     9361 tagctgcgcg gttggcgaat acaaaagcga gttgagcggc gcggatatca tcatcgcttc
     9421 tacgcacatt gcgggcgaaa tcaccgtgac cggcaacaaa tacgtggttg gcgtgcgcaa
     9481 catgctctct cctgccgact ttggcccgaa actgctggaa gtgatcaaag cgcatttccc
     9541 gcaggatgtg aagtaaggac gcgccatgaa attacatgat tcgctggcgg agaataaatc
     9601 catccgcctg caggctgaag ctgagacatg gcaggacgca gtaaaaatcg gcgttgacct
     9661 gctggtggcg gcggatgttg tcgagccgcg ttactaccag gcgattctgg atgcggttga
     9721 acagcacggt ccgtactttg tgctcgcacc aggcctggca atgccgcacg gtcgcccgga
     9781 agagggcgtt aagaaaaccg gttttgcgct ggtgacgctg aaaaagccgc tggagttcaa
     9841 ccacgaagat aacgacccgg tggatatcct catcaccatg gcggtggtcg atgccaacac
     9901 gcaccaggaa gtgggcatca tgcagatcgt caacctgttt gaagatgaag aaaatttcga
     9961 ccgtttacgt gcctgccgta ccgagcagga agtactggat ctcattgacc gcaccaacgc
    10021 ggcagcttaa gaaggaattg aagatgtcat taccgatgtt gcaagtcgcg ctggacaacc
    10081 agactatgga tagcgcctac gaaaccactc gcctgattgc cgaagaagtc gacattatcg
    10141 aagtgggtac cattctgtgc gtgggcgaag gcgtgcgtgc ggttcgcgat ctgaaagcgc
    10201 tctacccgca caaaatcgtg ctggcagacg ccaaaattgc cgatgcgggc aaaattcttt
    10261 ctcgtatgtg cttcgaagcc aacgctgact gggtgaccgt gatttgttgt gcggatatca
    10321 acaccgccaa aggtgcgctg gacgtagcaa aagagtttaa cggcgacgtg cagatcgaac
    10381 tgaccggtta ctggacctgg gaacaggcgc aacagtggcg cgacgcaggt attcagcagg
    10441 tggtttatca ccgcagccgt gacgcgcagg ccgctggcgt ggggtgaagc cgatatcact
    10501 gcgatcaaac gtctttccga tatgggcttc aaagtcaccg tcaccggtgg cctggcgctg
    10561 gaagatctgc cgctgttcaa aggcattcca attcatgtgt ttatcgccgg tcgcagcatc
    10621 cgtgatgccg cttctccggt tgaagccgcg cgtcagttca aacgttccat cgccgaactg
    10681 tggggctaag gagcggatat gttgtccaaa caaatcccgc ttggcatcta tgaaaaagcg
    10741 ctccccgccg gggagtgctg gctggaacgc ctgcgactgg caaaaacgtt gggcttcgat
    10801 tttgtcgaga tgtcggtaga tgaaactgac gagcgcctgt cgcgcctcga ctggagccgc
    10861 gagcagcgtc tggcgctggt gaatgcgatt gttgaaaccg gcgtgcgtgt gccgtccatg
    10921 tgcctttctg cccatcgtcg tttcccgttg ggcagcgaag atgacgcggt gcgggcgcag
    10981 gggctggaga ttatgcgtaa agccatccag ttcgcccagg acgttggcat tcgcgtgatc
    11041 cagctgcggg ctatgacgtt tactatcagg aagccaataa cgaaacgcgt cgtcgtttcc
    11101 gtgacggcct gaaagagagc gttgagatgg cgagccgcgc acaggtgacg ctggcgatgg
    11161 agatcatgga ttatccgttg atgaactcca tcagcaaggc gctgggttac gcgcactatc
    11221 tcaacaaccc gtggttccag ctctacccgg atatcggcaa cctgtcggcg tgggacaacg
    11281 atgtgcagat ggagttgcag gccggaatcg ggcatatcgt cgcggtgcat gtgaaagaca
    11341 ccaaaccggg cgtcttcaaa aacgtgccat ttggcgaagg tgtggtggat ttcgaacgtt
    11401 gcttcgaaac gctcaaacag agtggctatt gcgggccgta cctgattgag atgtggagcg
    11461 aaacggcgga agacccggcg gcagaagtgg tgaaagcgcg catggccaaa gcaggcatgg
    11521 tggaggcggc ataatgcaaa agctaaaaca gcaggtgttt gaagccaaca tggatctgcc
    11581 gcgctatggg ctggtgacct ttacttgggg caacgtcagc gctatcgacc gcgaacgcgg
    11641 gctggtggtg atcaagccca gcggcgttgc ctacgaaacc atgaaagcag acgatatggt
    11701 ggtggtcgat atgagcggca aggtggtgga aggggagtat cgcccatctt ccgacactgc
    11761 gacgcatctc gaactctacc gtcgttaccc gtcgcttggc ggcattgtcc atacccactc
    11821 cactcatgcc accgcatggg cgcaggcggg gctggcgatc ccggcattag gcaccacgca
    11881 cgctgactac ttctttggtg atatcccgtg tacgcgtgga ttaagcgaag aagaggtgca
    11941 gggtgagtat gaactgaaca ctggcaaagt gattatcgaa acgctgggca acgccgagcc
    12001 gctgcatacg ctgggaattg tggtgtatca gcacggaccg ttcgcctggg ggaaagatgc
    12061 ccacgacgcg gtgcataacg cggtagtgat ggaagaagtg gcgaaaatgg cgtggattgc
    12121 gcgcagtatt aatccacaac tcaatcatat cgacagctac ctgatgaata aacacttcat
    12181 gcgtaagcac ggtcctaacg cttattacgg gcagaagtaa aacacgcgct gcggaaattt
    12241 ccttcctcgg gagataactg gtctaattcc gcagccgttt ttcaaaaaaa gccccctgcg
    12301 aagggggcaa agcaaactat ggcaatgttt cgttggttat acctggtgct agcgataaat
    12361 atccgcgctg gcgtgcatat tgccgttact ccccggttcc cgcatcagaa ttacgtggta
    12421 gtacgttgcg ccttgcgcat ctgtttcttc atttaatgcc tgacgtgctt cgctttcagt
    12481 ggcgaaatta tgattgatat aaatcacgcc taagctttgc acatcgtcca tatttctggc
    12541 ctggtggtta ttaatttcaa tggctgccca tgtatttgca cttagcaaaa gcacagccag
    12601 aagggctaaa actcgactga acatagatac ctcctcgacg gctgactttg tgtgctctcc
    12661 tctgtgatga tcttctgatt taattttaat caatgataaa gaagttgatg gtgaccattt
    12721 ctgatgcagt tgttcaaaaa aacactgtga cggaatgtga tgaacttcaa atcagcggtt
    12781 tagaggttat ttgcgaaagg ggagatttat ttctgctccg cccttgagtt tagcggggta
    12841 tgtcagtact ataacgcgtc atttttcagc cgacctttaa cacgttcctt gcctccccgg
    12901 gattcggctg acccagacag gaggctgaat aatccgtaag gagcaattcg atgcgtcatt
    12961 acgaaatcgt ttttatggtc catcctgacc agagcgaaca ggttccgggc atgatcgagc
    13021 gctacactgc tgccatcact ggtgcagaag gcaagatcca ccgtctggaa gactggggcc
    13081 gccgtcagct ggcttacccg atcaacaaac tgcacaaagc acactacgtt ctgatgaaca
    13141 ttgaagctcc gcaggaagtg atcgatgagc tggaaactac cttccgcttc aacgatgccg
    13201 ttatccgcag catggttatg cgtaccaagc acgctgttac cgaagcatct ccgatggtta
    13261 aagcgaaaga cgagcgccgt gagcgtcgcg atgatttcgc aaacgaaacc gctgatgatg
    13321 ctgaagctgg ggattctgaa gagtaatttc tgatgaccaa ccgtctggtg ttgtccggca
    13381 ccgtgtgcag gactcccctt cgaaaggtca gtccatcagg aattcctcac tgccagttcg
    13441 tgcttgagca tcgttctgtg caggaggaag ccggctttca ccggcaggca tggtgtcaaa
    13501 tgcccgttat tgttagcgga cacgaaaacc aggccattac tcacagtata acggtcggca
    13561 gtcgcataac cgttcagggg ttcatttcat gccacaaggc aaagaacgga ctgagcaaaa
    13621 tggttttgca cgccgagcag attgaattga tagattctgg agactagcca tatggcacgt
    13681 tatttccgtc gtcgcaagtt ctgccgtttc accgcggaag gcgttcaaga gatcgactat
    13741 aaagatatcg ctacgctgaa aaactacatc accgaaagcg gtaagattgt cccaagccgt
    13801 atcaccggta cccgtgcaaa ataccagcgt cagctggctc gcgctatcaa acgcgctcgc
    13861 tacctgtccc tgctgccgta cactgatcgc catcagtaat cggtcacggt ccattaatac
    13921 gactttgaga ggataaggta atgcaagtta ttctgcttga taaagtagca aacctgggta
    13981 gcctgggtga tcaggtaaac gttaaagcgg gctatgctcg taacttcctg gtaccgcagg
    14041 gtaaagctgt tccagctacc aagaaaaaca ttgaattctt cgaagcacgt cgcgctgaac
    14101 tggaagctaa actggctgaa gttctggcag ctgctaatgc tcgcgctgag aaaatcaatg
    14161 cactggaaac tgttaccatc gcgtctaaag ctggcgacga aggtaaactg ttcggttcca
    14221 tcggtactcg cgacatcgct gacgctgtaa ctgcagctgg cgttgaagtg gctaagagcg
    14281 aagttcgtct gccgaacggc gttctgcgta ccactggcga acacgaagtg agcttccagg
    14341 ttcacagcga agtattcgcg aaagtgatcg taaacgtagt agctgaataa ttcgttattc
    14401 aacgagacgt aaaaacgccg accattggtc ggcgttttgc tttctatttt tcgtcaggca
    14461 ttaatttcgc aagtagatcc attcaccatt ctcttgtgtc cataagtcag gatggtttgc
    14521 tggcgttaga tgcatacgat cactccttat gtctcggtcc tgcggcactc gctcgaaacc
    14581 taaacccgag tagattgcgg ctgaatttga taaggaacgt agaccaattt tcaaatcatg
    14641 gggtaaatca gaatacgcgt ttagcaatgc agcaatgatg aatccgaggg cattagaaac
    14701 gcctgtaaca gtgcgaagtt ggtcgatata aagatcgatt gttggtagtt cagatgacca
    14761 gcaattaggc atttgggcag gaagggttac aacgtatcct atcgtttggc cggtatagtc
    14821 tggagtacta taaccaggtc ccaaaacaac ctcgtgtatt gtgtatttac tgttgggttc
    14881 gaaggattcg acatcttgca ataaatattt cagttctgcg gttaacctcg cacccgcgta
    14941 agctttattg tatttctggg cgtgaatatt atctaatgta gcttcatggt tccatccaaa
    15001 agaattatca ccattcattg t
//
LOCUS       DADMEQ010000092        14926 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.093, whole genome shotgun sequence.
ACCESSION   DADMEQ010000092 DADMEQ010000000
VERSION     DADMEQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.093"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            82..363
                     /locus_tag="JNZ05_002903"
     CDS             82..363
                     /locus_tag="JNZ05_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566067.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHWKKPERLRR"
     gene            complement(1160..2620)
                     /gene="phoQ"
                     /locus_tag="JNZ05_002904"
     CDS             complement(1160..2620)
                     /gene="phoQ"
                     /locus_tag="JNZ05_002904"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HAY8566068.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(2620..3291)
                     /gene="phoP"
                     /locus_tag="JNZ05_002905"
     CDS             complement(2620..3291)
                     /gene="phoP"
                     /locus_tag="JNZ05_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HAY8566069.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(3460..4830)
                     /gene="purB"
                     /locus_tag="JNZ05_002906"
     CDS             complement(3460..4830)
                     /gene="purB"
                     /locus_tag="JNZ05_002906"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAY8566070.1"
                     /translation="MELSSLTAVSPVDGRYGDKLSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(4834..5475)
                     /gene="hflD"
                     /locus_tag="JNZ05_002907"
     CDS             complement(4834..5475)
                     /gene="hflD"
                     /locus_tag="JNZ05_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HAY8566071.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(5511..6617)
                     /gene="mnmA"
                     /locus_tag="JNZ05_002908"
     CDS             complement(5511..6617)
                     /gene="mnmA"
                     /locus_tag="JNZ05_002908"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAY8566072.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQRGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(6671..7132)
                     /gene="nudJ"
                     /locus_tag="JNZ05_002909"
     CDS             complement(6671..7132)
                     /gene="nudJ"
                     /locus_tag="JNZ05_002909"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HAY8566073.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQSQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDIG
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(7142..7795)
                     /gene="rluE"
                     /locus_tag="JNZ05_002910"
     CDS             complement(7142..7795)
                     /gene="rluE"
                     /locus_tag="JNZ05_002910"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HAY8566074.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            7967..9217
                     /gene="icd"
                     /locus_tag="JNZ05_002911"
     CDS             7967..9217
                     /gene="icd"
                     /locus_tag="JNZ05_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HAY8566075.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(9320..9643)
                     /gene="iraM"
                     /locus_tag="JNZ05_002912"
     CDS             complement(9320..9643)
                     /gene="iraM"
                     /locus_tag="JNZ05_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY8566076.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGRIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESEITNKCLHKSCIIKIC
                     PYGLK"
     gene            complement(10176..10286)
                     /locus_tag="JNZ05_002913"
     CDS             complement(10176..10286)
                     /locus_tag="JNZ05_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566077.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQAHTIKLIFEM"
     gene            complement(10339..10743)
                     /locus_tag="JNZ05_002914"
     CDS             complement(10339..10743)
                     /locus_tag="JNZ05_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HAY8566078.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNQLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(10964..11695)
                     /locus_tag="JNZ05_002915"
     CDS             complement(10964..11695)
                     /locus_tag="JNZ05_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY8566079.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRHYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFETRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            complement(11900..13111)
                     /gene="bluF"
                     /locus_tag="JNZ05_002916"
     CDS             complement(11900..13111)
                     /gene="bluF"
                     /locus_tag="JNZ05_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HAY8566080.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMTHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            13425..13661
                     /locus_tag="JNZ05_002917"
     CDS             13425..13661
                     /locus_tag="JNZ05_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HAY8566081.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSHCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            13704..13976
                     /locus_tag="JNZ05_002918"
     CDS             13704..13976
                     /locus_tag="JNZ05_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HAY8566082.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKLAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            14005..14271
                     /gene="ariR"
                     /locus_tag="JNZ05_002919"
     CDS             14005..14271
                     /gene="ariR"
                     /locus_tag="JNZ05_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HAY8566083.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            14384..14632
                     /locus_tag="JNZ05_002920"
     CDS             14384..14632
                     /locus_tag="JNZ05_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566084.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT         4006 a         3403 c         3474 g         4043 t
ORIGIN      
        1 atgaggattt tgcttttgat ataagcgaca gccaccgcca gaaactggaa gaatttagcg
       61 gggtgtaatg aggtcagcag catgacagaa gccgaaataa cgggattaat ccgccgcgtt
      121 gccggaatca gccagcaggc tgacgaacag gccacgcagc cggacagcgt gacagccgaa
      181 aactatgcgc gtgttgttgc tgaggtgatg cgccgtgatg gtatagaact taatggcgtg
      241 gatatgcgca acatacgaac cagagttctt gagttgctgg cctaccgtcg ccgcgtggag
      301 atgtatcggg agaaggagaa aatcacgtac cactggaaga agccggagcg gttacggcgg
      361 taactcactg agattaaagc aaagcgcaaa attgcgctgg ctacatccct cacatctgag
      421 gactgttaac cagcaggccg gaatatattc agaaactacg aaattttcgc agtttaatta
      481 ctcgtcagct taatcgctaa cccgctctga tattttcgga aacctcaatt tgaggaagtc
      541 ggcgaggtaa ctgtttgata ttcccgatag cgcaaaaatg cgcggtcggg cagaatgacc
      601 accacctcaa tcgtgacgga gagtgacaac cagtgacaca ccgtgacaaa aagacggttt
      661 aaccgttcat taaaaatcga cgtgattttg tgctttccgg atgacgtggc caacgtcatt
      721 ttttagcgaa aaattctgat taaggtcact ggctgaacgg tcataatgac cacgcagacc
      781 atcgaggcaa ccatagcgat catcgttacg gttggagctt ttccgccatt ggtggaaaag
      841 gtgcacatct ggatcatcat tacggtgatc aagggtatgt aaaacccacc agcctgatta
      901 aaggttaacc ggaaaaaaaa tccggtagcc ccgttatggg gatccccata tcgacattaa
      961 cgcccctcat gaattggtgc gcttccccct ggaaagatta cccgcctgta tatttcttgt
     1021 gtctatttgt tccacgttgt ttcatacagt gcaccgaacg gtgtagttaa tggtgtagtc
     1081 aatttgcgat ttttggcact ttttcaatga aaattgttta atcgaatcaa tgacttgatg
     1141 tagtggtaaa aggacatatt tattcatctt tcggcgcaga atgctggcga ccaaaaatca
     1201 cctccatccg cgcaccgccc agcatgctct ctccggcgac gattttaccc tcatattgct
     1261 cggtgatttc gcgggctacc gccagcccta caccttgccc agggcgtaaa gtatcaaccc
     1321 gttgaccacg gtcgaaaatg acctctcgct tgcttaatgg aataccgggg ccatcatcct
     1381 cgaccacaat atagagatgc tcgtcggttt gccttgcaga aatttcgaca aactcgaggc
     1441 aatatttaca ggcattatcc agcacgttgc ccatcacctc gacaaaatcg ttctgctcac
     1501 cgacaaagct gatctctggc gaaatatcga gagagatatt gacccctttg cgttgataca
     1561 ctttgttcag cgctgaggtg agattgtcca gcagtggggc gaccggatgc agctcgcggc
     1621 tgagcaatgt cccgccgcgc atactggcac gatgcaggta gtagccaatt tgctgtgaaa
     1681 tgcggctgat ttgctccagc attaccggct cagcatcact gacgctcatc ttttcactac
     1741 gcagagaacg cagcgtactt tgcagcaccg ccagtggcgt tttcagacta tgggtcaggt
     1801 cggtgagcgt cgtacggtat ttgtcgtaac gttcgcgttc actttttaac aatcggttca
     1861 ggtttcgtac cagactggtc agttctcgcg ttgtggctgg attgagcaat tcgcggttat
     1921 gttcttccag ttcgcggact tcttttgcca gggcttcgat ggggcgtaaa ctccaccagg
     1981 cggcgaccca cagcagcggg atcactaaca gcagattggc tgagagcaca tagataaacc
     2041 agctccagac catataggaa ctttttagct ccaccggaat ggtatccacc accacaatgg
     2101 ttaattttgg catccgcgat gttgccgggt agacgtttac cgccaccgag tgggtcatct
     2161 ctgcgtcgtc atcatcttcc cgcacttcct gcaactgttg ctgtatcgaa tgatctccac
     2221 tcagcaagag gctggtatcg ttaacatccg cttcaatttc atgaaaacca ttcgatttca
     2281 gccagtcagg ctggatcatt ttcatcagcc agggcacgtc acgttgcgcc cataaaagct
     2341 gcccgttctc atcataaatt agcgtcatgg tggggctttg cttgtcgata ttttcgggta
     2401 actcgacatg caatttattg ttttcccact gcgcaagggt atagaacaga ttgctctcgc
     2461 cacgtaacag ccgaaacgta gttttatcga aactgacgct ataaccgatc agcgcgacca
     2521 ttccgtaggc aagcgaaagc accagtacta ccgctgccgt tgccaacaga aaacgtaccc
     2581 gcagcgagag cgggaaaaaa agacgcagta attttttcat cagcgcaatt cgaacaggta
     2641 gccctggccg cgaacggtgg taatgacttc ttgcggatat tgtgcctgaa tttttttgcg
     2701 cagacgtccc atcagtacat caatggtatg gctttcccgc agctccgcat ccggatagag
     2761 ttgtagcatt aacgaatctt tgctgaccac tttgccatta ttgcgtatca acgtttccat
     2821 aatggtgtat tcgaacgcgg tcagtttgat cacttcgtca ttaatagata attcacggcg
     2881 agagagatca acctgaaacg gggggagcga aatgacctgt gaagccagac cgctattacg
     2941 ccgcattaat gcctgcattc gcgccatcac ctcttcaata tgaaacggtt tagtcacgta
     3001 atcatcagca ccggcactta atacttcgac tttgtcctgc cagctttcac gggcggttaa
     3061 taccagaatc ggcagtgaaa catcgttgct acgccagcgg cgaatcagtg acagaccgtc
     3121 ctcgtcaggc aatccgagat cgacaatcgc aatatccggt aaatgttcat tgagataata
     3181 atcggcttct ttggcatctt ctgcgtcatc gacctgatga ccagcatcct gaatctgaac
     3241 tttaaggtgg tgacgtaaca acgcattgtc ttcaacaacc agtacgcgca tttttatttc
     3301 tccctgtctt aattattaaa atagtgtaac gcgattatgt gtttatgggg gtaaacatta
     3361 aataaaccag cggggagggg aggtaaagtg aaaaaagaaa aagtggataa tcttaataag
     3421 caggccggac agcatcgcca tccggcactg atttgaggct tatttcagct catcaaccat
     3481 cgtgatagct cgaccaatat agttagccgg cgtcatcgct ttcaggcggg ctttctcttc
     3541 ttctggcaac gccagaccat cgataaactg cttcatgcct tcggcgtcaa cgcgcttacc
     3601 gcgagtaagc tctttcagct tctcgtacgg tttttcgatg ccatagcgac gcataactgt
     3661 ctggattggt tctgccagca cttcccagtt gtgatccagc tcatccagca gatggtcacg
     3721 gttcacttcc agtttgctca cgcctttcag ggtggattga tacgcaatca gcgcataacc
     3781 gatacccacg ccgaggttac gcagcacggt ggagtcggtc aggtcacgct gccagcggga
     3841 aaccggcagt ttgcttgcca gatgctgcaa taccgcgttg gaaaggccca gattcccttc
     3901 ggagttttcg aagtcgatcg ggttaacttt atgcggcatg gtggaagaac caatctcacc
     3961 agcaatggtt ttctgtttga agtggttaag ggcgatataa ccccagacgt cacggtcaaa
     4021 gtcgatcaga atggtgttga agcgcgcaac gcaatcaaac agttcggcaa tgtagtcgtg
     4081 cggttcaatc tgggtagtgt acgggttcca ttgaataccc agcgaggtga cgaactcttc
     4141 gctgaactga tgccagtcaa cttccgggta agcggcgatg tgggcgttat agttaccgac
     4201 cgcgccgttg attttgccga ggatctccac ctggttaagc tggcggtgct ggcgctccat
     4261 acggtaggcg acgtttgcca tctctttacc gatggttgac ggcgtggctg gctgaccgtg
     4321 ggtacgagac agcagcggga tatcgcggta ctgaacggcg agatctttaa tgccatcaat
     4381 cagttgacgc cagtacggca ggatcacttc atcacgcgcg gttttcagca ttaatgcgtg
     4441 ggagaggtta ttgatatctt ccgaagtaca ggcaaagtgg atgaattcag aaaccgcgtg
     4501 cagttccggg atctccgcca ctttttcttt caggaaatac tcaaccgctt taacgtcgtg
     4561 gttagtggta cgctcgatgg ttttgatgcg tgcggcatct tcttcgctga aattggcgac
     4621 aattgcatca aggtaaccga ttgcgtcggc agcaaaagca ggaacttcct tgatcgctgc
     4681 gtgcgcggcc agtttttgca gccaacgtac ttcaacttgt acacggaatt tcagcaaacc
     4741 atattcgctg aaaatcccgc gcagcgcgct gagtttatcg ccgtagcgtc catcgacagg
     4801 ggaaacggcg gtcagtgagg ataattccat agatcacaac tccggggtta aatgagcaag
     4861 aatttgtttt gcctgagtgg tcaggcgatt acgagaaaac atcagttgta gacgtccgcc
     4921 gccgacctgg tgccagagca cggcggcgcg aatgcctgcc agcagggttg cgcgaacttt
     4981 cgcctgcact tgtgggcttt gcagtacagc aggggaaccg gtgacctgaa tgcgcgggcc
     5041 aagcgggctg atcacatcaa catagatagc agccatcgcg ctcatcagcg tttcggactg
     5101 taaatcgaag tgttcgagct ggcgttgcag gccgttgatc cggttgccca gagtgtcgag
     5161 cgcgcctttc gctgaggaga gtttgcgctc aagcaccatc aagctgagtg tgtagcgggt
     5221 taattcggcg tttaagccct ggcaactgct ggcattgagc acgccgagca gggtttccag
     5281 cccgacgcgc aggttggctt cgctaccgcc aaaaaccgcc agcgtcgagc tggggttcat
     5341 atcaataata ctgttgagtg agacgtgtag cgcatcggca tcacaatgcc cctggtgagc
     5401 gagttgttgc accaggcgtg ccgactgaca aataccggcc agggcgaggg tgatgtcata
     5461 gtaattcttt gccacgttca ctgcttcctt gttttaagta aagataataa tcagaccggc
     5521 agcggcagac gctgctcaat aataccgcca ccgaggcaca cttcaccgtt atagaagacg
     5581 gcagactggc ccggcgtcac ggcggcaacc ggctcatcga aaatcacttt aatgcgatcg
     5641 tcgtccagcg ccttgacggt gcacggaatg tcggtctggc gatagcgggt ttttaccgtg
     5701 caacgcatag tgccggtgaa tggttcgcga tcgacccagt gcagctgctg ggcaatcaac
     5761 ccgacagaca tcagccgcgg gtgttcatgg ccctgagcga caaccagaat gttgttttcg
     5821 acgtctttgt ccaccacata ccacggttct tcggtgcctt ctttggtgcc accgataccc
     5881 agacctttac gctgaccgag agtgtgatac atcagcccct ggtgctcacc aatttcatcg
     5941 ccatcgacgg taatgatttt gcccggttgc gccgggagat aacggcccag gaactcgcgg
     6001 aatttacgtt cgccaatgaa gcaaatgccg gtagagtctt ttttcttcgc ggtgaccaga
     6061 ccaagatctt cagcaatctt acgcacctgc ggtttttcca gttcgccgac cgggaacagg
     6121 ctttgcgcaa tctgctcatg gctgagcgta taaaggaagt agctctggtc tttattgctg
     6181 tccagaccac gcagcaggcg gctcttgcca tcgacatcgg cccgacgtac gtaatgaccg
     6241 gtagcgatat aatcggcacc taaatcttcg gcggcaaatt cgaggaaggc tttaaatttg
     6301 atctccttgt tgcacagaat atccggattc ggcgtgcgac cggctttata ttcggcaagg
     6361 aacagttcga agacgttgtc ccagtactcg gcagcaaagt taacggtgtg cagttcaatg
     6421 ccgagcttgt cgcagaccgc ctgggcatca gccagatccg ctgccgctgt gcaatattcc
     6481 tcaccgtcgt cttcttccca gttcttcata aacaggcctt cgacctgata tccccgttgt
     6541 tgcaacagcc aagcagaaac ggaggaatcg acaccgccgg acatgccgac gattactttt
     6601 tttgcggttt cagacattgg atcactcacg acattgaact tcaaggcggc gtattctatc
     6661 acgcagcctt ttagatgaca ccctttgtaa aaggccagtt aaaatcgcca atcatctcca
     6721 gcggataacg ttgcccgctt tgataacaac gaatactttc cgccaccagc ggcgaacgaa
     6781 gatttgacgc ctgtaaaatt tcttcggcgc tgacccaacg gcagcagccg atatcgctgt
     6841 catgaggctg ggtcgggcat atttgctcaa gctcaatggc aaagaggaaa cgcaaaaacg
     6901 gcgttttatc tggcgcaatc cactgatgca tacgaataaa gtgttgcgac tgcgcgctga
     6961 tgccggtttc ttcccacagt tcacgggcgg cggcttccac taaggtttca tcggcttcca
     7021 gatgcccggc aggttggttc cataacgctt taccattaat cgtctcttca acgactaaaa
     7081 atttgccttc tgcgtgcacc acgcaagcaa cggtaacgtg cggtttaaac attttgcatc
     7141 ctcaatctgt cacttctcgc cattcgccat tggcaagatt atccaaagag taatctccca
     7201 tcgcatagcg aatcagtcgc agcgtgggga aaccaacatg ggcggtcatg cggcgcacct
     7261 ggcgattacg tccttcatat aaggtgatct tcagccagct ggtgggaata cttttgcgtt
     7321 cacgaattgg tggattccgc ggccataacc acgcgggttc gtcaaccagc tccgcgcctg
     7381 cgggcagggt agggccatca tttaaggtta cgccattgcg caaggcttca agtgcgtctt
     7441 gtgtgggaat accttccacc tgcacataat agatttttcc ggtgcgttta cccggctggg
     7501 ttaaacgcgc ctgcaacgcg ccgttattgg tcagcaccag caacccttcg ctatcgcggt
     7561 caaggcgacc tgctgcataa acgccctgaa ccgggatgaa ttcttttaat gttttgcgtc
     7621 cggcttcatc ggtgaactgc ggaagaacat cgtagggttt attgaagagg atcacacgcg
     7681 tgggctggtt ttcatgttta cgcctggtag aacgttgcga gctgaatcgc ttaacctggt
     7741 gatttctaaa agaagttttt tgcatggtat tttcagagat tatgaattgc cgcattatag
     7801 cctaataacg cgcatctttc atgacggcaa acaatagggt agtattgaca agtcaattac
     7861 aaatcattaa caaaaaattg ctctaaagca tccgtatcgc aggacgcaaa cgcatatgca
     7921 acgtggtggc agacgagcaa accagtagcg ctcgaaggag aggtgaatgg aaagtaaagt
     7981 agttgttccg gcacaaggca agaagatcac cctgcaaaac ggcaaactca acgttcctga
     8041 aaatccgatt atcccttaca ttgaaggtga tggaatcggt gtagatgtaa ccccagccat
     8101 gctgaaagtg gtcgacgctg cagtcgagaa agcctataaa ggcgagcgta aaatctcctg
     8161 gatggaaatt tacaccggtg aaaaatccac acaggtttat ggtcaggatg tctggctgcc
     8221 tgctgaaacc cttgatctga ttcgtgaata tcgcgttgcc attaaaggcc cgctgaccac
     8281 tccggttggt ggcggtattc gttctctcaa cgttgccctg cgtcaggaac tggatctcta
     8341 catctgcctg cgtccggtac gttactatca gggcactcca agcccggtta aacaccctga
     8401 actgaccgat atggttatct tccgtgaaaa ctcggaagac atttatgcgg gtatcgaatg
     8461 gaaagctgac tctgccgacg ccgagaaagt gattaaattc ctgcgtgaag agatgggcgt
     8521 gaagaaaatt cgcttcccgg aacattgcgg tatcggtatt aagccgtgtt ctgaagaagg
     8581 caccaaacgt ctggttcgtg cagcgatcga atacgcaatt gctaacgatc gtgactctgt
     8641 gaccctggtg cacaaaggca acatcatgaa gttcaccgaa ggcgcgttta aagactgggg
     8701 ctaccagctg gcgcgtgaag aatttggcgg tgaactgatc gacggcggcc cgtggctgaa
     8761 agttaaaaac ccgaataccg gcaaagagat cgtcataaaa gacgtgattg ctgatgcatt
     8821 cctgcaacag atcctgctgc gtccggctga atatgatgtt atcgcctgta tgaacctgaa
     8881 cggtgactac atttctgacg ctctggcagc gcaggttggc ggtatcggta tcgcccctgg
     8941 agcaaacatc ggtgacgaat gcgccctgtt tgaagccacc cacggtactg cgccgaaata
     9001 cgccggtcag gacaaagtaa accctggctc tattattctc tccgctgaga tgatgttacg
     9061 ccatatgggc tggactgaag ccgcagacct gattgttaaa ggtatggaag gcgcgatcaa
     9121 cgccaagacc gtaacctatg acttcgagcg tctgatggaa ggcgctaaac tgctgaaatg
     9181 ttcagagttt ggtgatgcga tcatcaagaa tatgtaatca ctacatgtgt taaatattta
     9241 aacgggcgta taacacgccc gttgttttat ttatgtggat attattaata gcatatcgag
     9301 catatttata taaaacctat tacttgagcc catatgggca tatttttata atacaactct
     9361 tatgcaaaca tttatttgtt atttcgcttt ctcctggagg acactcttga ctgcttttga
     9421 gtaaactcca taaattcttg ttgaatggtg caatgttata aatagtaata ggatattctt
     9481 tttccttaag gataatacca agcttaaccg gtgtaaatat ccttccagga ggaagaaata
     9541 tagtagattg ataccagatg atcattttca tatcacccca tatggctgaa aaagatatgc
     9601 cgcatgtagg ttgaattacc gtgtcaatta ctacccactt catttgttat gtcttatccc
     9661 acggtattta atatagttca tttggatgtt catttcttta ttttgcatat gagtatatta
     9721 ccccttcaaa aaataaatta attaaaacga ttgcttatat aaaacaaaat ttaaatcaag
     9781 gaatctcaat ggatgttaaa caaaatgaga tttagtgaaa acaataaatt attcacctcg
     9841 ttttagattt gtttagctat aatgttatac attcaaatga atgaacatcc tgtaattaaa
     9901 acatagcctt tatgctactt ggtgccaatt tgctaaacat tatggttgcc tttttatata
     9961 acgataataa tgaatataaa catgacatga gaataaggtt tcaatttttg agttatatag
    10021 gaaaggttta acctgttcct ggctaaaata catataactg gatgatgact aaaccaaaac
    10081 acatatgcgt taagtattga aacgggcgta tggcacaccc gttgttttta taaatatatt
    10141 aaccgttata aaataacgta taaaaagtca agtgatcaca tttcaaatat caatttgata
    10201 gtatgggctt ggtgattatt tatgggtagc aataaaagga cagtatttat catccatagg
    10261 gatagtctct gtacttttat tcccattatg ctaatgcctt actgaattat gaagcatttc
    10321 ttaagcatcc aactttagct agattaatgg tttattattt tctacatctt caatatataa
    10381 aagcgtatta tcaatggcgt agtaactgcg tttgttatga ttaacatcag taacccaacg
    10441 gaaaacgccc gcgcctgcta gtgttgaaca gtattcccga aatgtagatt ttccgcaaat
    10501 atgaagcaat gcggcctctt ttattttagc aggattcttg gtcgtactaa cttttaactg
    10561 gttcctggtt cctcttaata acaaaaccgt gtcatcgtga gtaataattc tgatgttatc
    10621 cgtagccaga taataaatgt aatgtgcaat acggtgatgt tttaattctg aataaaacca
    10681 ggagaagttt tgctcttttc tcacttgctc aaacatcttt tgaaaaacaa cgacctgatc
    10741 catcaggata ataacctctt gttagttgtg agactgcgta gtgtgcacga tcggttttac
    10801 cacttcaatc tggtctgtcc tttggctgtg atatgtacag agtgtgatag agggattacc
    10861 tgaattctcc cggtgagcat tttgcaacgg accagctccg gtacaaatgc tgttgtgggt
    10921 tcagattata acattctgtc taaggggcgg gataaaggtg aaattagggg gcatgaaaga
    10981 tgactttata accttgctca ccccaatgtt gtaaaagttc gttttgcctt ctcgttggtg
    11041 ccatgcctgt ccagacaatc aatgtttgcg tcgggaacag ttccgggcgc ggcgattcaa
    11101 tgggatcagc aagaacagaa atgtgccatc ctgacaatga taaacgccat gcttccagcc
    11161 acaatcgtgc tctctcttca acattccacg ccatcagaat ggcttcttta ccgggcttac
    11221 ggcgcgtttc gaaaagcgag gttgctgcgt attcaattaa tgcgccgtca aacatactgc
    11281 tcataatgcg ggaggtgttg tgatcaagca cgagacgctg gcgaacagga aggtaaacat
    11341 gattaatcaa ttgatctact gggtactctc gacccagtga aataattctc gcgcgtagtt
    11401 tggcaggatt agccatacga agaattgaca tcatctcttc ttgcaggcgg ctccagtcat
    11461 cttccgtatc ctggctggtg gtttccagta atgctttaac tttgcctaca gggacgccat
    11521 tactgatcca acgcttgatc tcttcgatgc gttgtatgtc ttcttcatca aagagtcggt
    11581 gtccgccttc actgcgctgt ggttttaaca aaccgtagtg gcgttgccag gcccggagag
    11641 tgacaggatt aatcccgcaa cgttcagcaa catcaccaat gctgtaataa gccacaattc
    11701 ctccttgcgg tcacaaatct ccgtcgcctg tacacgaccc aataatactt tgtacaatat
    11761 acgctaaaat tgtacaaagt ataaataaga tttcagctaa attggatgaa acattatttt
    11821 taatgtagat taaatttaaa cataacgtat tcattttcac gatgatttac tgaaatcatg
    11881 tgaaagaatg tgctgaaaat tattttttct ctggccacgc tatggaaggg ataccattca
    11941 atttagcttt agcaaacaga tctccctgaa acatctcaat tcctgcggat tcaagccaca
    12001 tccactcttc tggtgttgcc acgcccatag cactgacttg aatttcaagt gatgtacagc
    12061 attttatgat cgcttgaata attgcctgcc gtggcccgct tttatgaaca ttggtaatca
    12121 attcctgact gattttaatt ctgtcaggct ggaagcgtga caggagtaac aaaccggcaa
    12181 aacctgcgcc aaaatgatca attgctacac tgataccggc agccttaagc gatttaatcg
    12241 cttctgcaaa ctcatcaaac cgagatataa cttcactttc agtaaattca acgatgattt
    12301 gttcaggcac cagagcattg gcctttattt catcaagtaa aaaagagact gcgtcaggtt
    12361 cgttaaccag ggtcataggt aatagattga ttgaaatcat tttatcaccg agctcaagtg
    12421 cgtgtgtcat cgcgaatgca agcgccttac ttttgagatc cgctgtgtag atttccccgt
    12481 ctttacgctg cccaaccgct atggctgatg ggctatcttc atttttttgc acaatggctt
    12541 caaaagcgat tatccgccgc gataagggat cgacaatagg atgaaaggca aagtggtcgt
    12601 ttatagttgg tgaaagggta caggaatcaa gttctttatc agatccgtca gcgataaaaa
    12661 gccaggagtc ttcggcaggg atctcgaaat aggttgattg ttcggttgca aggacaaaag
    12721 tacgaaaaaa ttgtagcgct ctgtcatcat aagttagctg aaattttgat gtgcctttgt
    12781 cgaatacggc ctgtaaaacg tcatctcgct cgtgcaggcg caaatcaaat aattccattc
    12841 ccgctttgcc aaaacggcga gcaggcgcgt aatcgcacag cagctcaaca atattatagt
    12901 gccgtggatc ttggcatata gcccgatata tcattttaac ctgttcttcc ggaccttcca
    12961 gaagctggaa aaaatgagaa ccattaaaca gtaagatccc tgttacgtca gactgcatgt
    13021 tcctgcgatt tgctatcgaa accatttctt cgattttttt gacaggttcg tcgtcacgta
    13081 tatggctacg ataaataagg gtggtaagca ttaacaatcc agggtaatgg gtgaggcgag
    13141 agtaagacgg taacagacat atcttcttgt gtctttcttt taataccaaa acataaccgt
    13201 ttctttacat tgataaaaaa tggaaaaagt tgaacacgag ttggcgaaaa atcttgtata
    13261 gattgtcagt taaatgatgc aatatgtttt atcataacac attgttttat atacattagt
    13321 actaattgca aaaaattaat ttatcattct gtacacatat ttcgtacaag tttgctattg
    13381 ttacctcact tcacattgat taacattttt aacagaggcg tagcatgcat caaaattcag
    13441 tgacttcaga ttctgcggga gcaatcactc gttactttgc aaaagctaac ttgcctactc
    13501 agcaggaaac tctcggggaa attgtgactg agattttgaa agatggacgt aatctgagtc
    13561 gaaagtcgca ttgtgccaaa cttctttgcc gactggaaca agcgacaggg gaagaggaac
    13621 agaaacatta taatgcacta attgggttgc tttttgaata atgcaacagc ctgattattt
    13681 aacaggttag ctatgaagtc gttatgaaga catctgataa tgaacgtata aaatatgaaa
    13741 ttactggcca ggcggtgctc cagatactgc gcatgaagat aaatttttca ttgcagactc
    13801 ttattaagca attactcgta atgaaattgg ctgaggaaga tgctttccga cgcgatttaa
    13861 ttgacagcat aattcgtgat tttagcaata gtgattcagg agggccaaac cgaagaacag
    13921 cgacagctga caataaaagt atgttcaatg gtaagaaaat aaacagaata cattaaaatt
    13981 tcataagtaa gatgagaggt taccatgctt gaagatacta caattcataa tgcaataact
    14041 gataaagcgt tagcaagtta ctttcgcagt tcgggtaatt tgttagaaga agagtcagca
    14101 gtgttagggc aggctgtcac caatttaatg ctttcaggcg ataatgttaa taataaaaat
    14161 attatcttaa gtctgataca ctccctggaa acaacaagtg atattctcaa agctgatgtg
    14221 attagaaaaa cactggaaat cgtgttgcga tacacagctg atgatatgta acttctaatt
    14281 attaagtata agtttataga aaactcattc atcttatttt tgtctgtcgc tttagactat
    14341 acacagcata attttattgg gttaatattt ctacgaggct gacatgaata attcaatccc
    14401 agagagattt atttttcaat gtgctttgtt taaaaatctc gagagggagg tgttcatgac
    14461 gcatgggtat gttgatagtc atattattga tcaggcttta cgcttgcgtt taaaagatga
    14521 aaccagtgtg attctttctg atctctatct gcaaatattg cagtacattg aaatgcataa
    14581 gactacacta acggatatca ttattaatga cagggaatcc gtgctctctt agaacttgag
    14641 taaggtttta gaataaacat cagctgtatc accatgctga tgcaaagtga gcatttcagg
    14701 cgttatgctt tcttattatg tccgcaatat caggtgtcaa gaatggagag ttctcgctct
    14761 ccattcttga cgcctgatat cccgcctaac ttattttgta ctgcctacat tatgctcaat
    14821 aattaatggg ttagatcaag atggagaaaa ttttatcttc ggcgtggata tttcttatgc
    14881 tctctaccat gcatctctgt agatgccagc gcgagcgaat gaatgg
//
LOCUS       DADMEQ010000093        14925 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.094, whole genome shotgun sequence.
ACCESSION   DADMEQ010000093 DADMEQ010000000
VERSION     DADMEQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.094"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            597..794
                     /locus_tag="JNZ05_002921"
     CDS             597..794
                     /locus_tag="JNZ05_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566085.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="JNZ05_002922"
     CDS             complement(744..1937)
                     /locus_tag="JNZ05_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8566086.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="JNZ05_002923"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="JNZ05_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HAY8566087.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="JNZ05_002924"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="JNZ05_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HAY8566088.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="JNZ05_002925"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="JNZ05_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HAY8566089.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAEVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7800
                     /gene="iucD"
                     /locus_tag="JNZ05_002926"
     CDS             6484..7800
                     /gene="iucD"
                     /locus_tag="JNZ05_002926"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HAY8566090.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSPASLTHYTANIQE"
     gene            7806..10001
                     /gene="iutA"
                     /locus_tag="JNZ05_002927"
     CDS             7806..10001
                     /gene="iutA"
                     /locus_tag="JNZ05_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HAY8566091.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            10945..14832
                     /gene="sat"
                     /locus_tag="JNZ05_002928"
     CDS             10945..14832
                     /gene="sat"
                     /locus_tag="JNZ05_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HAY8566092.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPEETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSRTSHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
BASE COUNT         4020 a         3553 c         3708 g         3644 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatggctcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg tcgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagaa gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccc tgcttcttta actcactata cagcaaatat tcaggaataa
     7801 cgatgatgcg caaaaagtat atgccccggg ctcttggtcc gctgcttctt gtcgtgctgt
     7861 caccagctgt cgcccagcaa aacgatgata atgagatcat agtgtctgcc agccgcagca
     7921 atcgaactgt agcggagatg gcgcaaacca cctgggttat cgaaaatgcc gaactggagc
     7981 agcagattca gggcggtaaa gagctgaaag acgcactggc tcagttaatc cccggccttg
     8041 atgtcagcag ccaaagtcga accaactacg gtatgaacat gcgtggccgc ccgctggttg
     8101 tcctgattga cggtgtgcgc ctcaactctt cacgttccga cagccgacaa ctggactctg
     8161 tcgatccttt taatatcgac catattgaag tgatctccgg cgcgacggcc ctgtacggtg
     8221 gcgggagtac cggagggttg atcaacatcg tgaccaaaaa aggtcagccg gaaaccatga
     8281 tggagtttga ggctggcaca aaaagtggct ttaacagcag taaagatcac gatgagcgca
     8341 ttgccggtgc tgtctccggc ggaaatgacc atatctccgg acgtctttcc gtggcatatc
     8401 agaaatttgg cggctggttt gacggtaacg gcgatgccac cctgcttgat aacacccaga
     8461 ccggcctgca gcactctaat cggctggaca tcatgggaac cggtacgctg aacatcgatg
     8521 aatcccggca gcttcaactg ataacgcagt actataaaag tcagggagac gacaattacg
     8581 ggcttaatct cgggaaaggc ttttccgcca tcagcgggag cagcacacca tacgtcagta
     8641 aggggctgaa ttctgaccgc attcccggca ctgagcggca tttgatcagc ctgcagtact
     8701 ctgacagtga tttcctggga caggaactgg tcggtcaggt ttactaccgc gatgagtcgt
     8761 tgcggtacta cccgttcccg acggtaaatg cgaataaaca ggcgacggct ttctcctcgt
     8821 cacagcagga taccgaccag tacggcatga aactgactct gaacagccaa ctgatggacg
     8881 gctggcaaat cacctggggg ctggatgctg agcatgagcg ctttacctcc aaccagatgt
     8941 tcttcgatct ggctcaggca agtgcttccg gagggctgaa caaccataag atttacacca
     9001 ccgggcgcta tccgtcatat gacatcacca atctggcggc cttcctgcaa tccagctatg
     9061 acattaatga tatttttacc gttagcggtg gcgtacgcta tcagtatact gagaacaggg
     9121 tagatgattt catcgactac acgcagcaac agaagattgc tgccgggaag gcgatatctg
     9181 ccgacgccat tcctggtggt tcggtagatt acgataactt tctgttcaat gctggtctgc
     9241 tgatgcacat caccgaacgt cagcaggcat ggttcaattt ttcccagggg gtggcattgc
     9301 cggatccggg gaaatattat ggtcgcggca tctatggtgc agcagtgaac ggccatcttc
     9361 ccctgacaaa gagcgtgaac gtcagcgaca gtaagctgga aggcgtgaaa gtcgattctt
     9421 atgaactggg ctggcgcttt atcggtgaca acctgcggac tcaaatcgcg gcatattact
     9481 cgctttccaa taagagcgtg gaaaggaata aagatctgac catcagtgtg aaggacgaca
     9541 ggcgccgtat ttacggcgtg gaaggtgcgg tggactacct gatcccggat actgactgga
     9601 gtaccggtgt gaacttcaat gtgctgaaaa ccgagtcgaa agtgaacggt caatggcaaa
     9661 aatatgacgt gaaggaatca agtccatcga aagcgacagc ttacattaac tgggcgccgg
     9721 aaccgtggag tctgcgtgta cagagcacca cttctttcga cgtaagcgat gcagagggta
     9781 acgatattaa tggttacact accgtcgatt ttatcagtag ttggcagctt ccggtgggaa
     9841 cactcagctt cagcgttgag aacctcttcg accgtgacta taccactgtc tggggacagc
     9901 gtgcacctct gtactacagc ccgggttacg gccctgcttc actgtacgac tacaaaggcc
     9961 ggggccgaac ctttggtctg aactactcag tgctgttctg acggggatgc ttttgcaaca
    10021 agggtatgct gtaaacaaca tggtccttgg tggccatgtt gtttacagcc agtataatat
    10081 acagccttaa caagatattc cgtctccgga atgggctgtt caccactgaa taacattatg
    10141 tctgttttat ggatctttga ctgaaattta tcgcatatcc tcatttcatt gtccgtctga
    10201 aaactaacct tacaagtaag cgtcgtctca gcaccgtctg gcagatcctg aaattcctga
    10261 gaggatgatc tgcgcccaat atcctgagcc ggaactttgc tgcaatgaag gccaacgaaa
    10321 aatgggggag tatgttatag agtacttgct acagggtaaa aagaggtacc tgtcgctggt
    10381 tctccgttca gtttaaccgg gagattttct cctgcgcctg tcataaagtc cggtaatgga
    10441 gattgttaat acgatggtgc acgatgcatc gtcagtgccc ggcccggagg atgccccgta
    10501 gctacatacg gatcagggtt ggcaatatcg aatggcaggc tataggcaaa gttaaagccc
    10561 catggagtag cacaaaatat gccgcacaaa ggaaacggtc tgaataacgc agtgatggag
    10621 aacttcttcg gcacactgca aaatcgcatc gtggtcgatc gctaattcgt cgtgaatcac
    10681 tgaacgatgt tccatgcgaa cagatattcg atcatatcga aatattaaac attacccctc
    10741 cccacattgg gcaaaaaaaa acaaaaaacg atcaattaac gatcaattag tattgattca
    10801 cataatctct catcattaat gagaacattg tcacatattt acaatataga actgttactt
    10861 ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata ctttgttcat
    10921 tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata tagtgctgcc
    10981 actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa aacaaaccga
    11041 aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc agcaaatatt
    11101 gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa aggtattttc
    11161 cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt ctctttccat
    11221 aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac cgcaatagga
    11281 ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc agaaactcag
    11341 gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga ttttgagatt
    11401 cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggaaga gaccaaccct
    11461 acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg ttcaaaaaaa
    11521 atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga atccaaaatc
    11581 tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga ggtcacccaa
    11641 tgggactcat acggcatgat gatttataaa aatgataaaa catttcgtaa tcttgaaata
    11701 ttcggagaca gcggctctgg agcatactta tatgataaca aactagaaaa atgggtatta
    11761 gtcggaacaa cccatggtat tgccagcgtt aatggtgacc aactgacatg gataacaaaa
    11821 tacaatgata aactggttag tgagttaaaa gatacctata gtcataaaat aaatctgaat
    11881 ggcaataatg taaccattaa aaacacagat ataacattac accaaaacaa tgcagatacc
    11941 actggtactc aagaaaaaat aactaaagac aaagatattg tgttcacaaa tgggggaaat
    12001 gttctgttta aggataattt ggattttggt agcggtggta ttatctttga cgaaggccat
    12061 gaatataaca taaacggtca gggatttaca tttaaaggag caggaattga tatcggaaaa
    12121 gaaagcattg taaactggaa tgcattgtat tccagtgatg atgttttaca caaaataggc
    12181 cccggtactc tgaatgttca aaaaaaacag ggggcaaata taaagatagg tgaaggaaat
    12241 gttattctta atgaagaagg aacatttaac aatatatacc ttgcaagcgg aaatggtaag
    12301 gtaatactaa ataaagataa ttcccttggc aatgatcaat atgcggggat attttttact
    12361 aaacgtggtg gcacgctaga tttaaatgga cacaatcaga cttttactag aattgccgcc
    12421 actgacgatg gaacaacaat aactaactca gatacaacga aagaagccgt tctggcaatc
    12481 aataacgaag actcctacat atatcatggg aacataaatg gcaatataaa actaacgcac
    12541 aatattaatt ctcaggataa gaaaactaat gcaaaattaa ttctggatgg tagtgtcaac
    12601 acaaaaaatg atgttgaagt cagtaatgcc agtcttacca tgcaaggcca tgcaacagag
    12661 catgcaatat tcagaagcag aacgagtcat tgctccctgg tatttctttg tggaacggac
    12721 tgggtcaccg ttttgaaaga aacagagagt tcatataata aaaagttcaa ttctgattac
    12781 aaaagtaata atcagcagac ctcatttgat cagcctgact ggaaaaccgg ggtgtttaaa
    12841 tttgatacat tacacctgaa caatgctgac ttttcaatat cacgcaatgc caatgttgaa
    12901 ggaaatatat cagcaaataa atcagctatc acaatcggcg ataaaaatgc ttacattgat
    12961 aatcttgcag ggaaaaatat tactaataat ggttttgact tcaaacaaac tatcagtact
    13021 aatctatcca taggagaaac taaatttaca ggtggcatca ctgcacataa cagccaaata
    13081 gccataggtg atcaagctgt agttacactt aatggtgcaa cctttctgaa taatactcct
    13141 ataagtatag ataaaggagc aaaagttata gcacaaaatt ccatgttcac aacaaaaggt
    13201 attgatatct ccggtgaact gactatgatg ggaatccctg aacagaatag taaaactgta
    13261 acgccgggtc tccactacgc tgctgatgga ttcaggctga gtggtggaaa tgcaaatttc
    13321 attgccagaa atatggcatc tgtcaccgga aatatttatg ctgatgatgc agcaaccatt
    13381 actctgggac agcctgaaac tgaaacaccg actatatcgt ctgcttatca ggcatgggca
    13441 gagactcttt tgtatggctt tgataccgcc tatcgaggcg caataacagc ccccaaagct
    13501 acagttagca tgaataatgc gatctggcat ctaaatagcc agtcatcaat taatcgtcta
    13561 gaaacaaaag acagtatggt gcgttttact ggtgacaatg ggaagtttac aacccttaca
    13621 gtggacaacc ttactataga tgacagtgca tttgtgctgc gtgcaaatct ggcccaagca
    13681 gatcagcttg ttgtcaataa atcgttgtct ggtaaaaaca acctcctgtt agtcgacttc
    13741 attgagaaaa atggaaacag caacggactg aatatcgatc tggtcagcgc accaaaagga
    13801 actgcagtag atgtctttaa agctacgact cggagtattg gcttcagtga tgtaacaccg
    13861 gttatcgagc aaaagaacga tacagacaaa gcaacatgga ctctgatcgg ctataaatct
    13921 gtggccaacg ccgatgcggc taaaaaggca acattactga tgtcaggcgg ctataaagcc
    13981 ttccttgctg aggtcaacaa ccttaacaaa cgtatgggtg atctgcgtga cattaacggt
    14041 gagtccggtg catgggcccg aatcatgagc ggaaccgggt ctgccggcgg tggattcagt
    14101 gacaactaca cccacgttca ggtcggtgcg gataacaaac atgaactcga tggccttgac
    14161 ctcttcaccg gggtgaccat gacctatacc gacagccatg caggcagtga tgccttcagt
    14221 ggtgaaacga agtctgtggg tgccggtctc tatgcctctg ccatgtttga gtccggagca
    14281 tatatcgacc tcatcggtaa gtacgttcac catgacaacg agtataccgc aactttcgcc
    14341 ggccttggca ccagagacta cagctcccac tcctggtatg ccggtgcgga agtcggttac
    14401 cgttaccatg taactgactc tgcatggatt gagccgcagg cggaacttgt ttacggtgct
    14461 gtatccggga aacagttctc ctggaaggac cagggaatga acctcaccat gaaggataag
    14521 gactttaatc cgctgattgg gcgtaccggt gttgatgtgg gtaaatcctt ctccggtaag
    14581 gactggaaag tcacagcccg cgccggcctt ggctaccagt ttgacctgtt tgccaacggt
    14641 gaaaccgtac tgcgtgatgc gtccggtgag aaacgtatca aaggtgaaaa agacggtcgt
    14701 atgctcatga atgttggtct caatgccgaa attcgcgata atcttcgctt cggtcttgag
    14761 tttgagaaat cggcatttgg taaatacaac gtggataacg cgatcaacgc caacttccgt
    14821 tactctttct gataacagcc cgggccgcgt ttgcggccct tcttctaccg gagagaatat
    14881 gtattaccct gtgacagact atatcgctct tgctctcatt attag
//
LOCUS       DADMEQ010000094        14685 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.095, whole genome shotgun sequence.
ACCESSION   DADMEQ010000094 DADMEQ010000000
VERSION     DADMEQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.095"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            116..1417
                     /gene="rstB"
                     /locus_tag="JNZ05_002929"
     CDS             116..1417
                     /gene="rstB"
                     /locus_tag="JNZ05_002929"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HAY8566093.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            1493..2422
                     /gene="tus"
                     /locus_tag="JNZ05_002930"
     CDS             1493..2422
                     /gene="tus"
                     /locus_tag="JNZ05_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HAY8566094.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHEPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(2419..3822)
                     /gene="fumC"
                     /locus_tag="JNZ05_002931"
     CDS             complement(2419..3822)
                     /gene="fumC"
                     /locus_tag="JNZ05_002931"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HAY8566095.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSIKAGR"
     gene            complement(3965..5611)
                     /locus_tag="JNZ05_002932"
     CDS             complement(3965..5611)
                     /locus_tag="JNZ05_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HAY8566096.1"
                     /translation="MSNKPFHYQSPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            5810..6985
                     /gene="manA"
                     /locus_tag="JNZ05_002933"
     CDS             5810..6985
                     /gene="manA"
                     /locus_tag="JNZ05_002933"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAY8566097.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELGFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            7086..8594
                     /locus_tag="JNZ05_002934"
     CDS             7086..8594
                     /locus_tag="JNZ05_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY8566098.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTQGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8820..10085)
                     /gene="uidC"
                     /locus_tag="JNZ05_002935"
     CDS             complement(8820..10085)
                     /gene="uidC"
                     /locus_tag="JNZ05_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HAY8566099.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYVAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(10124..11497)
                     /gene="uidB"
                     /locus_tag="JNZ05_002936"
                     /pseudo
     CDS             complement(10124..11497)
                     /gene="uidB"
                     /locus_tag="JNZ05_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            complement(11494..13305)
                     /gene="uidA"
                     /locus_tag="JNZ05_002937"
     CDS             complement(11494..13305)
                     /gene="uidA"
                     /locus_tag="JNZ05_002937"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAY8566100.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHGFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(13693..14283)
                     /locus_tag="JNZ05_002938"
     CDS             complement(13693..14283)
                     /locus_tag="JNZ05_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY8566101.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(14512..>14685)
                     /locus_tag="JNZ05_002939"
     CDS             complement(14512..>14685)
                     /locus_tag="JNZ05_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY8566102.1"
                     /translation="WSHYPEIEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQ
                     ILTVSGGGVQELN"
BASE COUNT         3684 a         3820 c         3497 g         3684 t
ORIGIN      
        1 atacatccgt ctgttccgga cattatcttc caaacaaggt cttatccagt tggcggttta
       61 cattgctcca cagtcgtgaa ttaatacgaa tagtattctg ttgagggcga tgccaatgaa
      121 aaaattgttt atccagtttt acctgttatt gtttgtctgc ttccttgtga tgtctctgct
      181 ggttgggctg gtgtacaaat ttaccgccga acgcgcgggc aaacagtcgc tggatgattt
      241 gatgaacagt tcgctgtatc tgatgcgcag cgaattgcga gagatccccc cacacgactg
      301 gggtaagacg ctgaaagaga tggatttaaa tctctctttc gatctgcgtg tcgagccact
      361 gagtaaatac catcttgatg atatttccat gcaccgactg cgtggcggcg aaattgtcgc
      421 cctggacgat cagtacacgt ttttgcagcg tatcccgcgc agccactacg tgctggcagt
      481 tggtcctgtt ccttatcttt attacctcca tcaaatgcga ttgctggata tcgccctgat
      541 cgcttttatt gctatttccc tcgcctttcc ggtgtttatc tggatgcgtc cgcactggca
      601 ggatatgtta aaactggaag cagcggcgca acgatttggc gatgggcatc tcaatgaacg
      661 tatccacttt gatgagggtt cgagctttga acgacttggt atcgcattta accagatggc
      721 ggacaatatc aacgccttaa ttgccagcaa aaaacagctt attgacggta tcgctcacga
      781 actgcgaaca ccgttagtgc gcctgcgtta tcgactggag atgagcgata acctgagcgc
      841 cgccgaatcc caggcgttga atcgtgatat cagtcaactt gaagctttaa ttgaagagct
      901 gctgacttat gcccgactcg atcgcccaca aaacgagctt catcttagcg aaccagacct
      961 gccgttgtgg ctgtcaacgc atctggcaga tattcaggca gtaacgcccg ataaaacggt
     1021 acggataaaa acgctcatgc aaggccatta tgcggcgttg gatatgcgct taatggagcg
     1081 cgtgctggat aatttgctca ataacgccct gcgctactgc cattcaacgg ttgaaaccag
     1141 cctgctactg tcggggaata gagcgacatt aattgttgag gatgatggcc cagggattgc
     1201 cccagaaaac cgcgaacata tctttgaacc ttttgttcgc ctcgatccca gccgggatcg
     1261 ctcaaccggc ggctgcgggc tggggctggc aattgtccac tctatagcac tggctatggg
     1321 cggtacggtt aattgtgaca tcagcgaact gggtggtgct cgcttctcgt ttagctggcc
     1381 gttatggcat aacatcccgc aatttacctc tgcctgacac tacgcgcacg atggtcaagt
     1441 cacaacgact gtgctataaa ataagtatgt tgtaactaaa gtggttaata ttatggcgcg
     1501 ttacgatctc gtagaccgac tcaacactac ctttcgccag atggaacaag agctggctac
     1561 atttgccgct catcttgagc aacacaagct attggttgcc cgcgtgttct ctttgccgga
     1621 ggtaaaaaaa gaggatgagc ataatccgct taatcgtatt gaggtaaaac aacatctcgg
     1681 caacgacgcg cagtcgctgg cgttgcgtca tttccgccat ttatttattc aacaacagtc
     1741 cgaaaatcgc agcagcaagg ccgctgtccg tctgcctggc gtgttgtgtt accaggtcga
     1801 taacctttcg caagcagcgt tggtcagtca tattcagcac atcaataaac tcaagaccac
     1861 gttcgagcat atcgtcacgg ttgaatcaga actccccacc gcggcacgtt ttgaatgggt
     1921 gcatcgtcat ttgccggggc tgatcaccct taatgcttac cgcacgctca ccgttctgca
     1981 cgaacccgcc actttacgct ttggttgggc taataaacat atcattaaga atttgcatcg
     2041 tgatgaagtc ctggcacagc tggaaaaaag cctgaaatca ccacgcagtg tcgcaccgtg
     2101 gacgcgcgag gagtggcaaa gaaaactgga gcgagagtat caggatatcg ctgccctgcc
     2161 acagaacgcg aagttaaaaa tcaaacgtcc agtgaaggtg cagccgattg cccgcgtctg
     2221 gtacaaagga gatcaaaaac aagtccaaca cgcctgccct acaccactga ttgcactgat
     2281 taatcgggat aatggcgcgg gcgtgccgga cgttggtgag ttgttaaatt acgatgccga
     2341 caatgtgcag caccgttata aacctcaagc gcagccgctt cgtttgatca ttccacggct
     2401 gcacctgtat gttgcagatt aacgcccggc ttttatactg ccgaccatct gttctggccg
     2461 tacccagctg tcaaactcgg cttcgctaag ataccccagc gcaagggccg cagcttttaa
     2521 ggtcagccct tctttatgcg cttttttggc gatctccgcg gctttgtcat aaccaatgtg
     2581 ggtgttaagc gcagtcacca gcatcagcga ttcattgagt aattgattga ttcgctcacg
     2641 attcggttca atacccactg cgcagtgttt gttaaaactt tccatgccat ctgccagcaa
     2701 gcgcaccgat tgcaggaaat tatggatcac catcggacgg aagacgttca gttcaaagtt
     2761 accggaagcg ccacccatgt tgatcgccac gtcgttcccc attacctgac agcagagcat
     2821 ggttaatgct tcacactggg ttgggttcac tttccccggc atgattgagc tgcccggctc
     2881 attttccggg attgagattt caccaattcc gcagcgcggg ccagaggcca gccagcggac
     2941 atcattggcg attttcatca gtgacgcagc caaccctttc aacgcgccgt gcgcctgaac
     3001 cagggcatca caggtcgcaa gcgcttcaaa tttgttcggc gcggtaacaa acggtgcaca
     3061 ggtaatgact gccagttcat ctgctacgcg ccgcgcatac tccggatggg tatttagtcc
     3121 agtacccacc gctgtaccgc caagagccag ttccgctacg tgaggcaggc tgtattcgat
     3181 atgtttgaga ttatgctcga gcatcgctac ccagccggaa atctcctgcc ctagtgttag
     3241 cggcgtggcg tcctgcaagt gggttcgacc gattttgacg atatcggcaa atgcacgcga
     3301 tttttcactc agcgtctgtg tcagggtttt aagctgcgga atgagttgct tgcgcagtgc
     3361 cagcagcgcc gcaacgtgca tcgccgtcgg aaagacatcg ttggaacttt ggcttttgtt
     3421 cacgtcgtcg ttagggtgaa ctttacgttc catcccgcgc acaccgccga gtaattcact
     3481 ggcccggtta gccagcactt cgttcatgtt catgttactt tgcgtgccgg agccggtctg
     3541 ccagatagcc agcgggaatt cgtcgtcatg ctgtcctgcc agtacttcat ccgccgcctg
     3601 ccgaatggcg ctcgctttct cttcagacaa caagtctaaa tcttcattaa cttttgccgc
     3661 tgcgcgcttg gttagcgcca gcgcatgaat cagtgaggtg ggcattttct ccgtcgaaat
     3721 gcggaaatgc tccagcgagc gttgagtttg tgcgccccac agcttatctg ccgggacatc
     3781 aatcgccccc atcgaatctt tttcgctgcg tactgtattc atggcctgct cctcacctga
     3841 ttaatttttt ctttctgttt tgctttcgtt aagcaacttt tagctcactc attatttact
     3901 atttgataac aaatgtttgg tctttcgtgc catgtaaaaa aaccgccccg aagggcggct
     3961 ctgtttattt cacacagcga gtgcactgtg tgagttgtat ctgctggaag aagtcatttc
     4021 ctttatcatc cacaaggata aacgccggga aatcttccac ttcaattttc cagatggctt
     4081 ccattcccag ttccggatat tcaacacatt ccaggctctt aatacttccc tgcgccaata
     4141 cagcggccgg accaccgata ctgccaaggt agaagccgcc gtgtttttta caggcatccg
     4201 tcacctgctg gctgcggttg cctttcgcca gcatgatcat acttccgccc tgcgcttgca
     4261 gttgatcgac ataagaatcc atccgtccgg cggtcgttgg gccaagagaa ccggaggcat
     4321 aaccttccgg cgttttggcc ggacccgcgt agtaaatcgg atgatcttta atgtattgcg
     4381 gcagcccttc accgttatcc atccgctctt tcagtttggc gtgagcaata tcacgaccaa
     4441 cgataatcgt gccgttaagc gataagcgtg tagaaacggg atactgcgac aactgtgcga
     4501 ggatctcttt catcggacgg ttaaggtcaa cgcgcaccgc ttcgccttct cccgctttac
     4561 gcagctcttc agggatatat ttacctggat tatgttccag tttttcgatc cagatcccct
     4621 gacggttgat cttcgctttg atattacggt cagcagagca ggaaacaccc ataccaaccg
     4681 gacaggatgc gccgtgacgt ggcaggcgaa tcacgcggat gtcgtgagcg aagtatttac
     4741 caccaaactg cgcacccaga ccgagatttt gcgcttcgat cagtaattct ttttccagtt
     4801 ccacatcgcg gaacgcctga ccgtgctcat tcccttccgt tggcagttca tcatagtatt
     4861 tcgcggaagc cagtttcacc gttttaaggt tcgtttctgc agaagttcca ccaataacga
     4921 acgcaatatg atacggagga caggccgcgg tacccagcgt gcgcatcttc tcaaccaggt
     4981 aatttttcag tttccccggc gtcagtaacg ctttggtttc ctgatagaga tacgtcttgt
     5041 ttgccgaacc accacctttt gcgatacaga ggaatttgta ttcgtcgcca tcaacggcat
     5101 aaagatcgat ctgcgctggc agattggtgc cggtattcac ttctttatac atatccagcg
     5161 gcgcgttttg cgagtagcgc agattatctt cgatataagt gttatagaca ccgcgcgcca
     5221 gcgccgcttc atcaccacca ccggtccata cacgctgccc ttttttacca acaataatcg
     5281 cggtgccggt atcctgacag gttggcagaa cgcctttcgc cgcgatgtcg gagttacgca
     5341 ggaattgcag cgccacatat ttatcatttt cgctggcctc cgggtcacgc agaatgtcgg
     5401 ccacctgttg ttggtgagcc ggacgcagca taaacgacgc atcatgaaac gcctgacgcg
     5461 ccaacagagt taacgcttcg ggtgcgactt tcaaaatctc ctgcccttca aattcagata
     5521 cgctaacgtg ttcactggtt agcaggtaat actcagtatc atcttttttg agtggaaaag
     5581 gagactgata atgaaagggt ttgtttgaca ttgttctctc acttactgcc tggtttggtt
     5641 atgctctggg cgggtgttcc gttgccctgt taaaagcgag taacaatatc ctacacagtt
     5701 ttttaacaaa aactgagact agtacgactt tttgcggctc caggttactt cccgtaggat
     5761 tcttgcttta atagcgggat taatttccac attaaaacag ggattgatca tgcaaaaact
     5821 cattaactca gtgcaaaact atgcctgggg tagcaaaacg gcgttgactg aactttatgg
     5881 tatggaaaat ccgtccagcc agccgatggc cgagctgtgg atgggcgcac atccgaaaag
     5941 cagttcacga gtgcagaatg ccgccggaga tatcgtttca ctgcgtgatg tgattgagag
     6001 tgataaatcg actctgctcg gagaggccgt tgccaaacgc tttggcgaac tgcctttcct
     6061 gttcaaagta ttatgcgcag cacagccact ctccattcag gttcatccaa acaaacacaa
     6121 ttctgaaatc ggttttgcca aagaaaatgc cgcaggtatc ccgatggatg ccgccgagcg
     6181 taactataaa gatcctaacc acaagccgga gctggttttt gcgctgacgc ctttccttgc
     6241 gatgaacgcg tttcgtgaat tttccgagat tgtctcccta ctccagccgg tcgccggtgc
     6301 acatccggcg attgctcact ttttacaaca gccacatgcc gaacgtttaa gcgaactgtt
     6361 cgccaacctg ttgaatatgc agggtgaaga aaaatcccgc gcgctggcga ttttaaaatc
     6421 ggcgctcgac agtcagcagg gtgaaccgtg gcaaacgatt cgtttaattt ctgaatttta
     6481 cccggaagac agcggcctgt tctccccgct gttactgaat gtggtgaaat tgaatcctgg
     6541 cgaagcgatg ttcctgttcg ctgaaacacc gcacgcttac ctgcaaggcg tggcgctgga
     6601 agtgatggcg aactccgata acgtgctgcg tgcaggtctg acgcctaaat acattgatat
     6661 tccggaactg gttgccaatg tgaaattcga agccaaaccg gctaaccagt tgttgaccca
     6721 gccggtgaaa caaggtgcag aactgggctt cccgattcct gtggatgatt ttgccttctc
     6781 gctgcatgac cttagtgata aagaaaccac catcagccag cagagtgccg ccattttgtt
     6841 ctgcgtcgaa ggcgatgcaa cgttgtggaa aggttctcag cagttacagc ttaaaccggg
     6901 tgaatcagcg tttattgccg ccaacgaatc accggtgact gtcaaaggcc acggccgttt
     6961 agcgcgtgtt tacaacaagc tgtaagagct tactgaaaaa attaacatct cttgctaagc
     7021 ttattaaagg cttataacac cttcaggcgg ccagtccgcc tgatttcatt ttatggataa
     7081 tcattatgaa taaatcgctg gtagcggtag gcgtcattgt tgcgctaggc gtagtctgga
     7141 caggcggcgc atggtataca ggcaagaaga ttgaaaccca tctcgaagac atggtcgcgc
     7201 aggcgaacgc gcaactcaaa ctgacagctc ctgaatccaa cctggaagtg agttatcaaa
     7261 actatcatcg cggcgtattc agcagccagt tgcaactgtt ggtgaaaccc attgccggga
     7321 aagaaaatcc gtggattaaa agcggtcaga gcgtcatctt caacgaatcg gttgatcatg
     7381 gtcccttccc gcttgcccag cttaaaaaac tgaacctgat cccgtcgatg gcatcaattc
     7441 aaaccacgct ggttaataac gaagtaagca aaccactgtt tgatatggca aaaggtgaaa
     7501 cgccttttga gattaactcg cgcattggtt acagcggtga ttccagttcc gatatttcgc
     7561 tcaatccact gaattacgag caaaaggatg aaaaagtcgc ctttagcggc ggcgagttcc
     7621 agttaaatgc tgacagagac ggcaaagcca tctccctttc cggggaggcg caaagtggtc
     7681 ggatagacgc agttaacgaa tacaaccaga aagtgcagtt gacctttaat aatctgaaaa
     7741 ccgacggttc cagcacgcag ggaagttttg gtgagcgcgt aggaaaccaa aaactgtcac
     7801 tggaaaaaat gaccatttca gtggaaggca aagaactggc actgctggaa ggcatggaga
     7861 tcagcggtaa atcggatctg gtcaatgacg gtaaaacgat caatagccaa ctggattact
     7921 cgctaaacag cctgaaggta cagaatcagg atctgggcag cggcaagctg actttaaaag
     7981 tcggccagat tgatggtgaa gcctggcatc agtttagcca gcaatataac gcgcaaactc
     8041 aggcgctgct ggcacagcca gaaattgcca acaatcccga actttatcag gagaaagtga
     8101 cggaagcctt ctttagcgcc ctgccgctga tgttgaaagg cgatccggtg attactatcg
     8161 cgccgctaag ctggaaaaac agtcagggtg aaagtgcgct gaatctgtcg ctgttcctga
     8221 aagatccggc aacgactaaa gaagcgccgc aaacgctggc gcaggaagta gatcgttcgg
     8281 ttaaatctct ggatgcgaaa ctgaccattc cggtggatat ggcaactgag ttgatgactc
     8341 aggtagcgaa gctggaaggt tatcaggaag atcaagcgaa aaaactggcg aaacagcaag
     8401 ttgaaggtgc atcagcaatg gggcagatgt tccgtctgac caccttgcag gacaatacca
     8461 tcaccaccag cctgcaatat gctaacggtc agataacgtt aaatgggcag aaaatgtcgt
     8521 tagaagattt tgttggcatg tttgcgatgc cagctcttaa cgttccggct gtaccggcaa
     8581 ttccgcagca gtaattcacg gccaaaacgc cggagaattt tcactccggc gttttcatct
     8641 gaaaacaaac cagagattgc ctgatgcgct acgcttatca ggcctacctt ttcctgcaac
     8701 actttgaatt tatgagtttt tgtaggctgg ataaggcgtt tacgccgcat ccggcataaa
     8761 aaacgcgcac tttgtcaaca atctgaaacg ccggaggttt ttctctccgg cgttatttct
     8821 tacttcagca taaagtcata ctgaatactc attttcacat cccagtaatt caggcgatcg
     8881 cccagcgtac tgccatcgcg cttttcccgc cctttatcgg cagcaagaaa gaaattcatc
     8941 ccttgtaacg gcccttcgct gacccgatag ttcataccac ctaagatacc cgcgccttca
     9001 atccgcacat ctcgtttact ggattcatag ccgcgagtca caatcggtgc aaatgtcagc
     9061 gtaaattgcg gagttacacg gtagttgaca cctaattgcc aggactgcat tccttcacgg
     9121 ttacgatcaa gacttaatga aaagggatag ccaatgtcgg tatccaccac ggcaccattc
     9181 attgcatggc gcaaataacc accactgcca aatacgcttc cccacggcgc attccaggtc
     9241 aattgtccac taaccaacgc cgtttcattg ggctggctgg tattacgact taaaccttcg
     9301 agctgcgcgt aaaagccgag caactcccct ttcaacgtat aattcgggtc gagcggtatg
     9361 cttttctcga gaatactttg tatctgattg gaaacatctt tctgtaccga tgcccccaga
     9421 gaatagcgac tggtatcatc atgccaactg gcggctaaaa aagaacgtgc gcgatgttta
     9481 tcgtattttc ccgtctggct gttatacacc ccttcatcac gaataccggt tgttgaatca
     9541 gtccataaaa atactcgtga acgccacatt ccccacagat caatatttgg tgttaaagcc
     9601 caataaccta atgctccttc agaaactgtc ggtaatgtac gttgcgaact gccagccatt
     9661 aacggaatac gataaggtaa gctaccataa ctataatccg tcccgaagcg acctaattta
     9721 agcaggctat tttcactggg ttttattttt actgccccca gggcaaagcc aaaactttta
     9781 tcaccatcaa gataccaccg ggtactcata tcagcacgag cacctaattt ataaacataa
     9841 tacgctccgc cttcaacgcc aataatattg gaataataac cactattgaa atcgagcaat
     9901 cctccctgta cccatgcgta aatatctctt cccgcgccag cactgggctt atcagccctg
     9961 cgcaattcgt ttttcagtct gatgcgtagt cccgcttcat cgtcagccag ttgcgccacg
    10021 taagcagagg taaggccaga ggcagccgtc aggcaaataa cggccatggc cactattttt
    10081 ctcatagttg cacctttgat gttctgatta tttttattga atattaatta gtgatatcgc
    10141 tgattaattg ctgctgcact tttttacgat tatcaatttc aaccacgatt tctttgaatt
    10201 ttttatccgt gagcggataa aaccagataa taacgaatgc cagtagcata aatccgcaag
    10261 gtactaaggc aattgatgtg cggatgccca taataacttc cggcgtttac acctgattgg
    10321 cgatatatcc gcttaatccc aaaataaagg caggaattga acctccgatt gcctgaccgc
    10381 atttacgggt aaatgagaat agtgaatagg tgagcccttc aattcgcacg ccggtcaggt
    10441 attcaccgta ttctacggta tcagcttcca gtgcccacat cacagtcata gtaacgccct
    10501 gaccaattga agcgatggcc aacgcaacca acgccaccgg cagtgaccag acggaaaccc
    10561 agaagaatag cagataaccg caggttccca gcaaagcgcc aatcaggaag gtattctttt
    10621 taccgatcct cgcgaccatc cccggcacaa gcggtgccga tgccacagta ccaaccaggt
    10681 tttgcaccag taccagcaca gtgaacagcc cggtatcatt taacacatag cgcacgtaga
    10741 acaacgacga ggcgctgacc gcgaaggtcg aaatcagcac acacagcgca ccgatgcaca
    10801 acataaacag cgggcgattc cgtttcaggg tttgcaaact gatcttcaat gacggctgcg
    10861 ccacgatacg taccacattt tcacgcgtcg atttgaagca gatgaagtaa agcaccattc
    10921 cggcaatcgc cagcacaatc gtccagaaat ggtataccga caccatctct tccgggctgg
    10981 agttcttaat gctcggtcct atcagaaatg ccaggcatac aaaggtcaat gaagcggcaa
    11041 tcccacgagc cgcgcccaga cgggcgcgag attgtggttg ttgggtcatc gcggtagcaa
    11101 gtgaaccata aggaatattc accaggctgt agcaaagccc gaggcccatg taggtcaaat
    11161 atgcatacac cactttgcta ccatggctcc agtcggtcgg tacccagaat accagcacgc
    11221 tgaagatcat taacggcgca gtaccgaaga gtaaaaacgg gcggaatttt ccccagcggg
    11281 tattcacact gtccaccact cgtccggcaa agacgtcggc gaaggcatcg aatacccgca
    11341 ccagtaacag catggtgcct gccgcagcgg caccgacgcc agcgacgtcg gtgtagtaac
    11401 tcaataggaa gagcgccccc attgcgaagg cgaagttatt ggcgacgtca ccgaggctgt
    11461 agccgacgat ggtgcgccag gagagttgtt gattcattgt ttgcctccct gctgcggttt
    11521 ttcaccgaag ttcatgccag tccagcgttt ttgcagcaga aaagccgccg acttcggttt
    11581 gcggtcgcga gtgaagatcc ctttcttgtt accgccaacg cgcaatatgc cttgcgaggt
    11641 cgcaaaatcg gcgaaattcc atacctgttc accgacgacg gcgctgacgc gatcaaagac
    11701 gcggtgatac atatccagcc atgcacactg atactcttca ctccacatgt cggtgtacat
    11761 tgagtgcagg ccggctaacg tatccacgcc gtattcggtg atgataatcg gctgatgcag
    11821 tttctcctgc caggccagca gttctttttc cagtaccttc tctgccgttt ccaaatcgcc
    11881 gctttggaca taccaaccgt aataacggtt caggcacagc acatcaaaga gatcgctgat
    11941 ggtatcggtg tgagcgtcgc agaacattac attgacgcag gtgatcggac gcgtcgggtc
    12001 gagtttacgc gttgcttccg ccagtggcgc gaaatattcc cgtgcacctt gcggacgggt
    12061 atccggttcg ttggcaatac tccacatcac cacgcttggg tggtttttgt cacgcgctat
    12121 cagctcttta atcgcctgta agtgcgcctg ctgagtttcc ccgttgacag cctcttcgct
    12181 gtacagttct ttcggcttgt tgcccgcttc gaaaccaatg cctaaagaga ggttaaagcc
    12241 gacagctgca gtttcatcaa tcaccacgat gccatgttca tctgcccagt cgagcatctc
    12301 ttcagcgtaa gggtaatgcg aggtacggta ggagttggcc ccaatccagt ccattaatgc
    12361 gtgatcgtgc accatcagca cgttatcgaa tcctttgcct cgcaaatccg catcttcatg
    12421 acgaccaaag ccagtaaagt agaacggttt gtggttaatc aggaactgtt cgcccttcac
    12481 tgccactgac cggatgccga cgcgcagcgg gtagatatca cactctgtct ggcttttggc
    12541 tgtgacgcac agttcataga gataaccttc acccggttgc cagaggtgcg gattcaccac
    12601 ttgcaaagtc ccgctggtgc cttgtccagt tgcaaccacc tgttgatccg catcacgcag
    12661 ttcaacgctg atatcaccat tggccaccac ctgccagtca acagacgcgt ggttacagtc
    12721 ttgcgcgaca tgcgtcacca cggtgatatc gtccacccag gtgttcggcg tggtgtagag
    12781 cattacgctg cgatggatcc cggcatagtt aaagaaacca tggaagtaag actgcttttt
    12841 cttgccgttt tcgtcggtaa tcaccattcc cggcgggata gtctgccagt tcagttcgtt
    12901 gttcacacaa acggtgatac gtacactttt cccggcaata acatacggcg tgacatcggc
    12961 ttcaaatggc gtatagccgc cctgatgctc catcacttcc tgattattga cccacacttt
    13021 gccgtaatga gtgaccgcat cgaaacgcag cacgatacgc tggcctgccc aacctttcgg
    13081 tataaagact tcgcgctgat accagacgtt gcccacataa ttacgaatat ctgcatcggc
    13141 gaactgatcg ttaaaactgc ctggcactgc aattgcccgg ctttcttgta acgcgctttc
    13201 ccaccaacgc tgatcaattc cacagttttc gcgatccaga ctgaatgccc acaggccgtc
    13261 gagttttttg atttcacggg ttggggtttc tacaggacgt aacataaggg actcctcatt
    13321 aagataaaaa tactggtcaa cctttaatct gattagttaa ccaattctgt gttgcacaca
    13381 gttatctgtg agaaacccat ggaagagaaa tggaaataaa aagaactgac accgcgatca
    13441 aaaaaccaaa aactgcaata cccacagcgt gatatggatc gcattaatct ttaaaacagc
    13501 gttgacagaa gccataaaac aagcaatcga gcgttcgttc gagagtattg ggaacgataa
    13561 actctcatga catatggcta cggtgaatat tttggggggg aattctccga acacggggag
    13621 gataattttt gaacagagaa attacgatat gaaacgcggg cacagttatc accagcccgc
    13681 gaaatgagag gatcaggatg cggttaatat accgccaatc atcgcgcgta atccctgagc
    13741 aaacgacgct tcacgcgcca ggtcttccgc ttcgatatcg gccagtacac cgtaggtcag
    13801 atccagcagt aaacgtgaag tcattgccgt gttgatgtct gggcttatct cgcctttttg
    13861 ctgggcatca cgcatccgct gggcaacaaa ttcagtgatc gtcatatgct tatttttaag
    13921 catggcggcg acctgtgggt tacgcatccc ttccgccata atttcgacca ccagcgcccg
    13981 ttgcccaaag gcttcatggg tgagagagac aatagactcg accatatagt caacaaaatg
    14041 aatcccttca atcggttccc ggaaacgagc cagcgccctc tcctggtcct gtaagataat
    14101 cgcctgaatc aaggcttctt tggagatgaa atggtgatag agcgtcccag gactaatggc
    14161 gcaagattta cagatggctt tcatcgaggc actgtgaaat ccattttctg aaaaaatctc
    14221 tctggcagca ttgaggatcc gggtccgtgt cggttgtgct tcagtctgca tgttatccat
    14281 cattaaacca ttatagttag cgtgaataac tagtttaccg cgttgtcagg tgagcgccta
    14341 tcattgctgt tgaatagtgc gaaggtacac tctattcgta tacttttcac ccagcaattg
    14401 gttaaccaat tgccgtgatt tggctgccag ttgtttagca gagacgttga tgaattgcac
    14461 acgctatccc ggtaaacaac acatacgacc gtttaccggt tcgttagtgt attaattgag
    14521 ctcctgtacc ccaccaccgg agacggtgag aatttgtccg cttacccagc tggctgcagg
    14581 cgagcaaagg aacagcgccg cgttagcaat atcttgcggt tggcccagac gtctgatcgg
    14641 cgtgtgctgt aacatttttt gttcaatttc tgggtaatga ctcca
//
LOCUS       DADMEQ010000095        14685 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.096, whole genome shotgun sequence.
ACCESSION   DADMEQ010000095 DADMEQ010000000
VERSION     DADMEQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.096"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..145
                     /locus_tag="JNZ05_002940"
     CDS             <1..145
                     /locus_tag="JNZ05_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2645 domain-containing protein"
                     /protein_id="HAY8566103.1"
                     /translation="LLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLFWGRFI
                     FCY"
     gene            complement(191..1540)
                     /gene="qseC"
                     /locus_tag="JNZ05_002941"
     CDS             complement(191..1540)
                     /gene="qseC"
                     /locus_tag="JNZ05_002941"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HAY8566104.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTPDLNEINAADRMAQTPNKLKHDHVDDDALTFAIFTHDGRMLLNDG
                     DNGEDIPYSYQREGFADGQLIGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLLALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(1537..2196)
                     /gene="qseB"
                     /locus_tag="JNZ05_002942"
     CDS             complement(1537..2196)
                     /gene="qseB"
                     /locus_tag="JNZ05_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HAY8566105.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLSSDFIRTVHGIGYTLGE
                     K"
     gene            2348..2740
                     /gene="ygiW"
                     /locus_tag="JNZ05_002943"
     CDS             2348..2740
                     /gene="ygiW"
                     /locus_tag="JNZ05_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HAY8566106.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESVKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            2793..3275
                     /locus_tag="JNZ05_002944"
     CDS             2793..3275
                     /locus_tag="JNZ05_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8566107.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            3821..6079
                     /gene="parC"
                     /locus_tag="JNZ05_002945"
     CDS             3821..6079
                     /gene="parC"
                     /locus_tag="JNZ05_002945"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAY8566108.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNARAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            6313..7050
                     /gene="plsC"
                     /locus_tag="JNZ05_002946"
                     /gene_synonym="parF"
     CDS             6313..7050
                     /gene="plsC"
                     /locus_tag="JNZ05_002946"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HAY8566109.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            7125..8537
                     /gene="ftsP"
                     /locus_tag="JNZ05_002947"
     CDS             7125..8537
                     /gene="ftsP"
                     /locus_tag="JNZ05_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HAY8566110.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            8648..10867
                     /locus_tag="JNZ05_002948"
     CDS             8648..10867
                     /locus_tag="JNZ05_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HAY8566111.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDCVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSLINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(10910..11167)
                     /gene="yqhH"
                     /locus_tag="JNZ05_002949"
     CDS             complement(10910..11167)
                     /gene="yqhH"
                     /locus_tag="JNZ05_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HAY8566112.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(11218..12144)
                     /locus_tag="JNZ05_002950"
     CDS             complement(11218..12144)
                     /locus_tag="JNZ05_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HAY8566113.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKLIEAKTAARLKQ"
     gene            complement(12344..13171)
                     /gene="dkgA"
                     /locus_tag="JNZ05_002951"
                     /pseudo
     CDS             complement(12344..13171)
                     /gene="dkgA"
                     /locus_tag="JNZ05_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
     gene            complement(13276..14439)
                     /gene="yqhD"
                     /locus_tag="JNZ05_002952"
     CDS             complement(13276..14439)
                     /gene="yqhD"
                     /locus_tag="JNZ05_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAY8566114.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYKVAR"
BASE COUNT         3396 a         3727 c         3897 g         3665 t
ORIGIN      
        1 tcttcttacg ccacttatta ttcttatctt aagaaaactc tttatgcggg aaatgttcga
       61 gttttggcta tatgtattct cgctcgttat ttgtctggtt tgtggttggt ggcttttttg
      121 gggacggttt atattttgct attagtaaaa tctatgagag tcgttttaac ggctctcata
      181 gacagagaag ttaccagctt accttcgcct caaatccacc ttgttccgca ttcccaaact
      241 caacattcat gtcatgcagc ttggcgattc gctggacaat cgatagccca agtccactgc
      301 cggtagcggt ttgtccgggt gggcgataga agcgttcgcc aattcgcgcc agtgcctctg
      361 gtgtcacacc ggggccgtta tccctcacga tgaaattatc agcattcagc gtgacgtcta
      421 tcacgctgcc ctgtggactg tagcgcacgg cgttatccag caaatttcgc accaacaaac
      481 ttagcaatag cggttgtccg gtgcgtttga tgccgtgggc attgagtgtc agtcgcacgt
      541 caattttcgc ctgctgcgcc gtgtggtaaa tatccatcac cgacgattgt aggagatctt
      601 caagcgggat ctccgcgacg tcctgaaggt tatccagtga gtccagccgc gatagcgtga
      661 gcagttgatc aaccagacga gtagcgcgat cgatcccgga atgtaattgg agcagtgctt
      721 ttttccgcgc ctgcggatca tcgtcagaga gctgcgcaac ttcggtttgc actttcagcg
      781 ctgttaacgg gctacgtagt tcgtgagctg catcggaggt aaagcgtcgt tctcgaacca
      841 tcatcgcatg tgtgcgggcg aacagttgat ttagcgactc aaccagtggc cgcacttcgc
      901 tgggtacgcc agtcgcgttt agtggttttt ccgagtcagg gtcacgcata cgtagtgcca
      961 gcgccagttt gttcagcggc gcgagttcac gacccagtag taccatcatg atgattaaca
     1021 taatcggcag tgcgagcagc cacgggatca attgcccggc aacaatcgcc agcgccatgt
     1081 cttcacggta ttcccattcc tggccaacaa cgatgcgata tttgccatca ggtgaggtca
     1141 tccagacaaa acgccaagga tcgtcttcac cgatcagttg cccgtcagca aaaccttccc
     1201 gttgatagct atagggaata tcttctccgt tatcgccatc attaaggagc attctgccgt
     1261 cgtgggtaaa gatggcaaag gtcagcgcat cgtcatcaac atgatcgtgt tttaatttat
     1321 ttggcgtctg tgccatgcga tccgccgcgt tgatttcgtt gagatcgggc gtacttaacc
     1381 gtttggcaaa cagcatcagt tgggtgtcga acaattcatc gacgttatcc gttgtttgtt
     1441 tccaggcgac aaagctggaa agcagccagg tcaccgaggc cagaattaaa aagattagcg
     1501 tcagcctgac gcgcagacta agacgttggg taaatttcat ttctcaccta atgtgtagcc
     1561 aataccatgc acggtacgaa taaaatcact actgagtttg cgtcgcagat gatgcacatg
     1621 cacttcaacg gcattactgg tgacctcttc gtcccaggta tacagtttct cttcaatcag
     1681 tttgcgcggc agtacccgac cagcgttacg catcagtaat tccagcaggg caaattcttt
     1741 tggtttcagt gttaagggtt cgccagccag cgtggcgata cgtttgccgg ggtcgagcat
     1801 gacgttaccg tggcgcagct cgttgctggc ctggccgttg gttcggcgca tcagagcctc
     1861 cagcctggcg gcgacttcta tcaacgcaaa aggtttacac agataatcgt cagctcccag
     1921 acgcagccct tctacacgtt ccgctaacgc atcacgcgcg gtcaggttca gtaccggctc
     1981 acgctgacct ttctctcgcc attcgcgcaa aatatcgcga ccatccatgc ctggtaaggt
     2041 taaatccagg atcaccgcat cataaggtgc gctataaagc gcctcttttc cctgacgacc
     2101 ttgtgtaaac cagtcgacac taaaacccat tttactaagg ccagttttga tgccgtcacc
     2161 aatcagcatg tcatcttcta tcagtaaaat tcgcattttt tcatccctgc gataaccgta
     2221 tttgctgcca gtaaaccgtg ttgtaacgcg ctctgtacag cattaaattt tatttttttc
     2281 ccttaagaag ttgttaagga ctatcttgtt aaatgctcgg aaacagacat taaagggagt
     2341 aataaacatg aaaaaattcg cagcagtaat cgcagtaatg gccctgtgca gtgcaccggt
     2401 gatggcagca gagcagggcg gtttttctgg cccatcggca acgcaaagtc aggccggagg
     2461 atttcagggg ccgaacggca gcgtaacgac tgtagaaagc gtaaaatccc tgcgtgacga
     2521 cacctgggta accctgcgcg gcaatatcgt tgaacgcatc tctgacgatc tctacgtgtt
     2581 taaagatgcc agcggtacta tcaatgttga tatcgatcac aaacgctgga acggtgtgac
     2641 ggtgacgccg aaagatacgg ttgagattca gggtgaagtc gataaagact ggaattctgt
     2701 tgaaattgac gtcaaacaga tccgcaaagt aaatccgtaa ttgttaccgc tcccgggacg
     2761 cgttcccggg aataatttcg cagggaggca aaatgacaaa cctgacactg gatgtaaaca
     2821 ttatcgattt cccatcaatc cctgtggcga tgttgccgca ccgctgtagc cctgagttgc
     2881 tcaactacag tgtggcgaaa tttatcatgt ggcgtaaaga gacggggctt tctcctgtta
     2941 accaaagcca gacttttggc gtcgcctggg acgaccctgc caccaccgca ccggaagcgt
     3001 ttcgctttga tatctgcggc agcgttagcg aaccgattcc cgataatcgt tatggtgtga
     3061 gcaatggtga acttaccggt ggacgttatg ccgtggcccg ccacgttggc gagctggacg
     3121 atatttcaca cacgatatgg ggcattattc gccactggct gcctgcaagc ggcgagaaaa
     3181 tgcgtaaagc accgattctg tttcactaca ccaatcttgc cgaaggggtg acagagcagc
     3241 gactggaaac ggatgtttat gtgccgttgg cgtgatgggg aatctttacc ggagtgtgag
     3301 ccaaatcact cgcgataagt ttgagcgaac aatgtcacag gcgtattgtt gttgggctta
     3361 taatttctcc ggtaaggaat accatgtata cgcgaaattt attatccggt gcgccgtaag
     3421 ccgtctcggg ggtatccatg agcaagccgc acttatggca ggaggcgcgg ggcactggtg
     3481 gagacacagc taaactgggc tgaaccgtat acttacagtc gactactttg atgctttagg
     3541 cttccctgga ctcgcataac ctcaacttct cgaatcgccc ggagcggacc cccatgccga
     3601 gtggtgtggc atgggcgcaa ccttgtgctg ccctctatgc cgatcaagtc tacagcaaag
     3661 agttgtatat caaggcacat tgatgccttt atcgccgtcg gagcacgtcc ggcggcgcta
     3721 ttcacgctga tctcctgtga ctcgacgcgg cagataatgt agtatctccg gcaatattgc
     3781 ccctttgaag gctggcgaat aagttgagga atcagaatta atgagcgata tggcagagcg
     3841 ccttgcgcta catgaattta cggaaaacgc ctacttaaac tactccatgt acgtgatcat
     3901 ggaccgtgcg ttgccgttta ttggtgatgg tctgaaacct gttcagcgcc gcattgtgta
     3961 tgcgatgtct gaactgggcc tgaatgccag agccaaattt aaaaaatcgg cccgtactgt
     4021 cggtgacgta ctgggtaaat accatccgca cggcgatagc gcctgttatg aagcgatggt
     4081 cctgatggcg cagccgttct cttaccgtta tccgctggtt gatggtcagg ggaactgggg
     4141 cgcgccggac gatccgaaat cgttcgcggc aatgcgttac accgaatccc ggttgtcgaa
     4201 atattccgag ctgctattga gcgagctggg gcaggggacg gctgactggg tgccaaactt
     4261 cgacggcact ttgcaggagc cgaaaatgct acctgcccgt ctgccaaaca ttttgcttaa
     4321 cggcaccacc ggtattgccg tcggcatggc gaccgatatt ccaccgcata acctgcgtga
     4381 agtagctcag gcggcaatcg cattaatcga ccagccgaaa accacgctcg atcagctgct
     4441 ggatatcgtg caggggccgg attatccgac tgaagcggaa attatcactt cgcgcgccga
     4501 gattcgtaaa atctacgaga acggacgtgg ttcagtgcgt atgcgcgcgg tgtggaagaa
     4561 agaagatggc gcggtggtta tcagcgcatt accgcatcag gtttcaggtg cgcgcgtact
     4621 ggagcaaatt gctgcgcaaa tgcgcaacaa aaagctgccg atggttgacg atctgcgcga
     4681 tgaatctgac cacgagaacc cgacccgtct ggtgattgtg ccgcgttcca accgcgtgga
     4741 tatggatcag gtgatgaacc acctcttcgc taccaccgat ctggaaaaga gctatcgcat
     4801 taacctcaat atgatcggtc tggatggtcg tccggcggtg aaaaacctgc tggaaatcct
     4861 ctccgaatgg ctggtgttcc gtcgcgatac cgtgcgccgc cgactgaact atcgtctgga
     4921 gaaagtcctc aagcgcctgc atatcctgga aggtttgctg gtggcgtttc tcaacatcga
     4981 cgaagtgatt gagatcattc gtaacgaaga tgaaccgaaa ccggcgctga tgtcgcggtt
     5041 tggccttacg gaaacccagg cggaagcgat cctcgaactg aaactgcgtc atcttgccaa
     5101 actggaagag atgaagattc gcggtgagca gagtgagctg gaaaaagagc gcgaccagtt
     5161 gcagggcatt ttggcttccg agcgtaaaat gaataacctg ctgaagaaag aactgcaggc
     5221 agacgcgcaa gcctacggtg acgatcgtcg ttcgccgttg caggaacgcg aagaagcgaa
     5281 agctatgagc gagcacgaca tgctgccgtc tgaacctgtc accattgtgc tgtcgcagat
     5341 gggctgggta cgcagcgcta aaggccatga tatcgacgcg ccgggcctga attataaagc
     5401 gggtgatagc ttcaaagcgg cggtgaaagg taagagtaac caaccggtag tgtttgttga
     5461 ttccaccggt cgtagctatg ccatcgaccc gattacgctg ccgtcggcgc gtggtcaggg
     5521 cgaaccactc accggcaaat taacgttgcc gcctggggcg accgttgacc atatgctgat
     5581 ggaaagcgac gatcagaaac tgctgatggc ttccgatgcg ggttacggtt tcgtctgcac
     5641 ctttaacgat ctggtggcgc gtaaccgtgc gggtaaggct ttgatcacct taccggaaaa
     5701 tgcccatgtt atgccgccgg tggtgattga agatgcttcc gatatgctgc tggcaatcac
     5761 tcaggcaggc cgtatgttga tgttcccggt aagcgatctg ccgcagctgt cgaagggcaa
     5821 aggcaacaag attatcaaca ttccatcggc agaagccgca cgtggcgagg atggtctggc
     5881 gcaactgtac gttctgccgc cgcaaagcac gctgaccatt catgttggga aacgcaaaat
     5941 taaactgcgt ccggaagagc tacagaaagt cactggcgaa cgtggacgcc gcggtacgtt
     6001 gatgcgcggt ttgcagcgta tcgatcgtgt tgagatcgac tctcctcgcc gtgccagcag
     6061 cggtgatagc gaagagtaat atgccgggga gggcgctgtt tcctcccgct agcaaggaac
     6121 gccggatgaa acgttaagtc gtcatccggc agcgtattca acccccggaa acgataaaaa
     6181 ttcccttgaa accgttgttt attcatgcgt tgcgattaac aatacgcttt tccagagagc
     6241 ggcttttaac aatgccctaa cctgatttca ggtgacgtac aatgccagtc tctcagacct
     6301 tcagagggtg ttatgctata tatctttcgt cttattatta ccgtgattta cagcatctta
     6361 gtctgtgtat tcggctccat ttactgcctt ttcagcccgc gtaacccgaa acatgtggcc
     6421 acctttgggc atatgtttgg ccgtcttgcg ccactgttcg gcctgaaagt tgagtgccgc
     6481 aaacctgcag atgctgaaag ctacggcaat gctatctata tcgctaacca ccagaacaac
     6541 tatgacatgg tgacagcatc gaacattgtg caaccgccga cggtgacggt aggtaaaaag
     6601 agcttgctgt ggatcccctt cttcgggcag ttgtactggt taaccggcaa cttattgatc
     6661 gacagaaaca atcgcactaa agctcacggc accattgcgg aagtcgtgaa tcatttcaaa
     6721 aaacgccgta tttccatctg gatgttcccg gaaggaaccc gcagccgtgg tcgcggcctg
     6781 ctaccgttca agactggagc atttcacgcg gcaattgcgg cgggcgtccc gattattccc
     6841 gtgtgcgtct ctacaacttc gaataagatt aatcttaatc gactgcacaa cggtctggtg
     6901 attgtcgaaa tgctgccgcc aattgacgtc agtcagtatg gcaaagatca ggttcgtgag
     6961 ctggctgccc attgtcgttc gataatggaa caaaaaatcg ccgagctcga taaagaagtc
     7021 gcagaacgcg aagccgccgg aaaagtttaa gtcggcaatc tgtatttttg cggggaacac
     7081 tttcctgcac ggtattactt tagccagttt tacatggagc aaatatgtca ctcagtcggc
     7141 gtcagttcat tcaggcatcg gggattgcac tttgtgcagg cgctgttccc ctgaaggcca
     7201 gcgcagccgg gcaacagcaa ccgctacccg ttccgccgct acttgaatct cgccgtgggc
     7261 aaccgctgtt tatgactgta caacgtgcgc actggtcatt tacgccaggg acacgcgcgt
     7321 cggtctgggg aatcaatggt cgttacctgg ggccgactat ccgcgtctgg aagggcgacg
     7381 atgttaagct tatttacagc aaccgcctga cagaaaatgt ctcaatgacg gtggccgggc
     7441 tacaggtacc aggcccgctg atgggcggtc cggcacggat gatgtcgcca aacgctgact
     7501 gggcacccgt actgcctatt cgccagaacg cagctactct gtggtatcac gccaatactc
     7561 ccaaccgcac ggctcagcag gtctataacg gccttgccgg aatgtggctg gtggaagatg
     7621 aagtcagcaa gtcgctgcct atccccaacc attatggtgt ggatgatttt ccggtcatta
     7681 tccaggataa acggctggat aactttggta cgccagaata caacgaaccg ggaagcggcg
     7741 gctttgttgg tgatacgctg ctggttaacg gtgtacaaag cccgtacgtt gaagtctcgc
     7801 gtggctgggt gcgcttgcga ctgctgaacg cgtcgaactc tcgtcgctat caactacaga
     7861 tgagcgatgg tcgcccgtta catgtgattt ctggcgatca gggattcctg cctgctcctg
     7921 tatcggtgaa gcaactttcg ctggcaccgg gcgagcgccg cgagattctg gtggatatga
     7981 gcaacggcga tgaagtgtcg atcacctgtg gcgaagcggc gagcattgtt gatcgtattc
     8041 gcggcttctt tgagccatcc agtattctgg tttctacctt ggtgctaacg ctgcgcccaa
     8101 ccggccttct gccgctggtc acagacagtc ttccgatgcg cttgctgcca actgaaatca
     8161 tggctggttc gccaattcgc agtcgcgata tcagtctggg tgatgacccg ggtattaatg
     8221 gacagctgtg ggacgtcaac cgtattgatg tcaccgcgca gcaaggaacg tgggaacgct
     8281 ggacggtacg cgcggacgag ccgcaagcgt tccatattga aggcgtaatg ttccagatcc
     8341 gtaacgtgaa tggcgcgatg ccgttcccgg aagacagagg ctggaaagat accgtttggg
     8401 ttgacggaca agtggagctg cttgtttatt tcggtcagcc ttcctgggcg cacttcccgt
     8461 tctacttcaa cagtcagacg ctggaaatgg cggaccgtgg ctcgattggg caactgttgg
     8521 tcaatccggt accgtaatct tcatactcgc ccgaggaggg cgagtatgga atacctttcg
     8581 atttctcttt cgttccggct tataatcccc ggcctttgat tattttttta tcatttctgg
     8641 aagcaaaatg agctctatct ccctgatcca accggatcgc gacctgttct cctggccgca
     8701 gtactgggcc gcctgttttg gaccggcacc gtttttgccg atgtctcgtg aagagatgga
     8761 tcaacttggc tgggatagct gcgacatcat tttggttact ggcgacgcgt atgtcgatca
     8821 cccaagcttc gggatggcga tttgcggtcg tatgctggaa gcgcagggct ttcgcgtcgg
     8881 gatcatcgcc cagccggact ggagcagcaa agacgacttt atgcgtctgg gtaaaccgaa
     8941 tctgtttttt ggtgttactg ctggcaacat ggattcgatg atcaaccgct ataccgccga
     9001 tcgccgttta cgtcatgatg atgcctacac gccagataat gtcgcgggta agcgaccgga
     9061 tcgcgccacg ctggtttata cccagcgttg taaagaggcg tggaaagatg tgccggtgat
     9121 cctcggcggt attgaggcta gcctgcgccg taccgcgcat tatgattact ggtccgatac
     9181 cgtgcgccgt tccgttctgg tagattcgaa agccgacatg ctgatgtttg gtaacggtga
     9241 acgtccgctg gtggaagtag cgcatcgtct ggcgatgggc gagccgatta gtgaaatccg
     9301 cgatgtgcgt aataccgcaa ttatcgtaaa agaggcgctg cctggctgga gcggtgtgga
     9361 ttccacccgt cttgataccc cagggaaaat cgacccaatc ccgcatccgt atggcgaaga
     9421 tttgccgtgt gcggataaca aacctgttgc gccgaaaaag caggaagcga aagctgtaat
     9481 cgtgcagcca ccgcgccaga aaccgtggga aaaaacctac gtattgctgc cttctttcga
     9541 gaaagtgaag ggcgataaag tgctgtacgc ccatgcttcg cgcattctgc accacgaaac
     9601 caacccaggc tgcgcccgcg cattgatgca aaaacacggt gaccgctatg tgtggatcaa
     9661 cccgcctgct atcccgcttt ctaccgaaga gatggactgc gtttttgcgc tgccgtacaa
     9721 gcgcgtgcca catccggcct atggcaatgc ccgtattccg gcttacgaaa tgatccgttt
     9781 ttcggtcaac attatgcgcg gctgctttgg cggctgttct ttctgttcta ttaccgagca
     9841 cgaagggcgc attattcaga gccgttcgga agattcgctc attaatgaga tcgaagcgat
     9901 ccgcgacacc gttccaggtt ttacgggcgt gatttccgat ctcggtgggc caactgccaa
     9961 catgtatatg ttgcgctgca aatcgccacg cgctgaacaa acttgtcgcc gtttgtcgtg
    10021 cgtttatccg gatatttgtc cgcacatgga cactaaccac gaaccgacga tcaacctcta
    10081 tcgccgtgcg cgtgatctga aaggcattaa aaaaatcctt atcgcctcag gcgtacgtta
    10141 tgacatcgcc gtggaagatc cgcgctatat caaagagttg gcgacccatc acgtcggcgg
    10201 ttatctgaag attgctccgg aacataccga agaagggccg ttgtcgaaga tgatgaagcc
    10261 gggcatgggc agctatgacc gctttaaaga gctgttcgat acctactcga aacaggcagg
    10321 taaagagcag tatctgatcc cgtatttcat ctctgcgcac cccggtacgc gtgatgaaga
    10381 tatggtgaat ttggcgctgt ggctgaaaaa gcatcgtttc cgtctcgacc aggtacagaa
    10441 cttctaccca tcgccgctgg cgaactcaac caccatgtat tacaccggga aaaaaccgct
    10501 ggcgaagatt ggttataaga gcgaagacgt cttcgtaccg aagggcgaca aacagcgtcg
    10561 tttgcataaa gcgttgttgc gttaccacga tccggcaaac tggccgttaa tccgccaggc
    10621 gctggaagcg atgggcaaaa agcatctgat tggcagccgt cgcgattgct tagtgcctgc
    10681 gccaaccatt gaagagatgc gtgaagctcg tcgccagaac cgcaataccc gtccggcgtt
    10741 gactaaacat acgccgatgg cgactcagcg tcagacgcct gctacggcaa aaaaagcgtc
    10801 gtctacgcaa tctcgtccgg tgaatgctgg tgcgaagaaa cgccctaaag cggcggttgg
    10861 acgttaaaaa aaatccccgg caactgacat gctgccgggg atttttttat cattctgcgt
    10921 acatacgcaa gcagcgcagg caatcaaaat agtcctgtgc atcaagacgc gtattggctc
    10981 tgttagcttc ggatttggca gcatagattt gtgggcgtag tgctttcata tcctgctcaa
    11041 gccgggcgat tttggcattt aatgtttgta cattgctcgc cagttgattg accttttgct
    11101 cattgacgca gccactgagc aagcaggttg ccagaacaac agctcccacg gtgaaaatcg
    11161 ttttcatata gtgttccctt ttatttattg ttccggctaa ttgttattta attcagctca
    11221 ttgctttaat ctggcggcgg tttttgcctc aatcagctta agtaggctgc cgctgttcgg
    11281 gcggataaaa tcagcgattt cccacttacc ttcgcgccgc tgtaaaacca gctcttgctc
    11341 ttcattgttg ccattggtca gcgtaaaacg aacatgaatg cgggcggaat cggcggtttt
    11401 ctccaggact cgaatttcat caacgcgtga aaatagcgac tcttcgccaa aacagatcgg
    11461 attgtcgtaa ataaattgcc gttctatttg catatcttca tcgggattat tggccgtctt
    11521 gaaggtggtt ttatagaagg cattaaccgc ctggcggtaa gagtcggaaa ccacccacgt
    11581 ccacggccag ctataatcag caatatgttc aaagagttca gcaacaaagg attctggctg
    11641 ttctttctgc aacgaagcta aagcggccag cgttttttca ttctcgctat taactgcttg
    11701 taagacgctg ccatgattgc tgacaatatc atcaatgacc caacgaccat tttccgccac
    11761 cattttcagt gtctgtgtgg tcttttcttt atcatcttta aagatacgaa agcgcacaac
    11821 ggcatcggca tgatcgacgt cagtttgggt tatcgctacg ctttctaaca ccagatcgcc
    11881 aaaatcctga caatcacaaa ccggatcata atccagccag ccaatattgc ccggtagtgt
    11941 aagattgtcg ttcagggtaa gcgcctgttg aatacgcgct gaagttatcg cccgctctcc
    12001 ggtttcaccg aaataaggcg tactggcatc tgatttatag ttctgataaa tctgccggac
    12061 tgtttgttct acggtctgtg aaggaggctg cgcgtgtacg gtaaaacttg ccagggctgc
    12121 taaaaacaga agtattattt tcatctgttt ttcacctctc cgttgcgttt atcccggata
    12181 tagatgagtt gttcttttaa gagcttccgg ctctgcatga tgatgtcctt atatttggca
    12241 ttcctgaaca aggaagagta acaacgggcg ggacgcgagg ggaataaatg atttctgaaa
    12301 agtccggtag cggaacatta ccgccaccgg gagaatttgc acgttagccg ccgaactggt
    12361 caggatcggg accgagacgc ttgccctgat cgagttttgc aattttgccg agttcgtctt
    12421 tgtcgagacg gaaatcccag acatcaaagt tttcggcaat acgtgaaggt gtgaccgatt
    12481 tcgggatcac caccaggccg ctatccagat gccagcggat aacaatctgc gccggggttt
    12541 tgccgtattt atctgccaga tcgcgaatga ctttctgatc gaaaacgcct tttcctcctt
    12601 gcgctaatgg gctctaggat tcggtctgga ttttgtgtgt cgcgttccag gcgtgtagct
    12661 ggcgttgttg catcagcgga tgaagttcga tctggtttat cacaggcgtc acgccagttt
    12721 catcaatcag gcgttgcagg tgatggatct ggaagttgca cacgccgatg cttttgatta
    12781 atccctcttt ttgcaattcg atcatgcctt tccatgcttc gacataatgg tcgatagcgg
    12841 gaacgggcca gtgcattaag tagaggtcga tataatcaag ctggagtttt ttcaggctgt
    12901 cgagcagggc ttcgcggggg cacttgtggt cgtcgttcca cagcttagtg gtgatgaaca
    12961 gttcttctct gttgactgag gcatttttca gggctttgcc gacaccttct tcgttcttgt
    13021 aggccgcggc ggtatcaatc gagcgataac ccacttctaa cgctttttga atggcggtga
    13081 ttacttcctc attacttgct tgccagacgc ccagtcccag ctggggcatg acattgccat
    13141 cctgtagctt aataacggtt ggattagcca tacgttcctc ctttatatga actcaccgga
    13201 gcaggctccg gtgaggtgtt gtgaacttaa gtctggacga aatgcccgaa aacgaaagtt
    13261 tgaggcgtaa aaagcttagc gggcgacttt gtatatacgg cggctgacat ccaacgtaat
    13321 gtcatgattt tcgcccagtt gggtcatgcc gtgctcttcc agttttttca gcaaagccgg
    13381 gatggagctg ccgtccagac cgtagtcgga gaggtgggtc ggcacgccta attgctcaaa
    13441 gaaattgcgg gttgcggcaa tcgcggcgtc aatacgctca tcgtcggaac cttcagtgat
    13501 gttccagacg cgttcagcat attgcagcag cttagcgcgc ttggtctcgc gtttttcatt
    13561 ccacagtgca ggcaggacga tagccagtgt ttgcgcgtga tccagaccgt gcatcgcagt
    13621 cagttcgtgg cccagcatat gcgttgccca gtcctgcggt acgccagcgc caatcaaacc
    13681 gttcagcgcc tgagtcgccg cccacatgac gttggcgcgc acatcgtagt tttctggctc
    13741 tttcagggct ttcggaccat cttcgattag cgtcagcaaa atgccttctg cgaaacggtc
    13801 ctgaattttg gcatcaaccg gtttggtaac atactgttcc acggtgtgta caaaggcgtc
    13861 cactacgccg ttagccacct gacgcggcgg cagggtgtag gtataaaccg gatcgagcac
    13921 ggcaaatacc ggctgaacat gtgcagaatg gaacgcctgc ttgtcgcctg tggttttacg
    13981 ggagatcacc gcgcctgcgt tggattctga accggttgct ggcagcgtca gcacacagcc
    14041 catcgggatg gcgcttttaa tctctttacc gcccgtttgc agaatgtgcc acggatcgat
    14101 attttccgga tagttagccg ctgcggcgat aaatttggtg ccgtccagta cagaaccgcc
    14161 gccaaccgcc agcaggaaag tcactttctg ttcgcgaacc agtttcacgg cgttcatcag
    14221 cgtttcataa gccgggtttg gctcaatacc gccaaattcc agcacgtcca tgcctttcag
    14281 ggcatccaga acttgatcga gaacgccggt ttttttcacg ctgccgccac cgtaggtaat
    14341 caatacgcga gcatcgtgag gaatttgttc gcgtaaacca gcgattgcgc ctttaccaaa
    14401 cagaatgcgg gttggggtgt gcagattaaa gttgttcatt acttgctccc tttgctgggc
    14461 caatatgagg gcagagaacg atctgcctga tgtttttcat tgtgatcgcc agcgccctgg
    14521 ctctcaatgc tcatttctgc caatgtcttg cctatttctc cagagtgctg gagaaatgct
    14581 acaaaattgc gcacaatcaa attgccgcat tattcctaag aaattacgcg atatgaaacg
    14641 tgaagagatt tgtcgcttgc tggcggataa agttaataaa ctgaa
//
LOCUS       DADMEQ010000096        14559 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.097, whole genome shotgun sequence.
ACCESSION   DADMEQ010000096 DADMEQ010000000
VERSION     DADMEQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.097"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(1045..1713)
                     /locus_tag="JNZ05_002953"
     CDS             complement(1045..1713)
                     /locus_tag="JNZ05_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY8566115.1"
                     /translation="MSHPIINVGRYHGGSDDWRTPYRLFHNLHREFNFSLDGAATEYD
                     ALLPRFTDDIHNQSWVGERVFVNPPFSMAEKFLLKAPEADVCVCLVPLRSKTTYWLRC
                     VYTNPFLHEIRTLHRAVKYLPPAGKRGVVVRSPFPSCVLIYRNTPRKPKVEVTQTIYC
                     GDTMLLLAVVNRGGLRGRPHMHDAQTLDRLIKMYDQGQPIKSIAESLRIPLSTTYRIV
                     QRLG"
     gene            complement(3329..3835)
                     /locus_tag="JNZ05_002954"
     CDS             complement(3329..3835)
                     /locus_tag="JNZ05_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566116.1"
                     /translation="MDSIRLTGLKVRAAREEEKENAKIDADIKAEVERGKREELVTEE
                     LQTEKKLIQKEINNLKQLVSKEKQTIENIEIEKERLQDLIVASLEVMDDFFKVDNSRS
                     LQQLKSRVEELLTVSDIEASTIRNALRQKKELTSSQTMKMFDMVEAKIKKEKEKGNDI
                     ESDELINQ"
     gene            4253..4603
                     /locus_tag="JNZ05_002955"
     CDS             4253..4603
                     /locus_tag="JNZ05_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAY8566117.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            4656..5051
                     /locus_tag="JNZ05_002956"
     CDS             4656..5051
                     /locus_tag="JNZ05_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566118.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAMEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            5092..5835
                     /gene="cmoA"
                     /locus_tag="JNZ05_002957"
     CDS             5092..5835
                     /gene="cmoA"
                     /locus_tag="JNZ05_002957"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HAY8566119.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     NAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGSAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            5832..6803
                     /gene="cmoB"
                     /locus_tag="JNZ05_002958"
     CDS             5832..6803
                     /gene="cmoB"
                     /locus_tag="JNZ05_002958"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HAY8566120.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(6968..9310)
                     /locus_tag="JNZ05_002959"
     CDS             complement(6968..9310)
                     /locus_tag="JNZ05_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY8566121.1"
                     /translation="MALTIREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAAMLGQIGTPGGGFGFSYHYSNGGNPMRVGGVLPEMSAAIAGQASEAADD
                     GGMTAIPVARIVDALENPGGKYQHNGKEQTYPNIKMIWWAGGGNFTHHQDTNRLIKAW
                     QKPEMIVVSECYWTAAAKHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQFE
                     ARNDFDVFADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNAFW
                     QQNKLIEMRRSEKNEQYVRYGDFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPAHP
                     TWLAPDEWKGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDAAR
                     FGIANGDLVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVLTA
                     DIPSSQLANACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
     gene            complement(9335..10435)
                     /locus_tag="JNZ05_002960"
     CDS             complement(9335..10435)
                     /locus_tag="JNZ05_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HAY8566122.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(10823..11569)
                     /gene="cutC"
                     /locus_tag="JNZ05_002961"
     CDS             complement(10823..11569)
                     /gene="cutC"
                     /locus_tag="JNZ05_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAY8566123.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGGLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(11583..12149)
                     /locus_tag="JNZ05_002962"
     CDS             complement(11583..12149)
                     /locus_tag="JNZ05_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY8566124.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            12365..14098
                     /gene="argS"
                     /locus_tag="JNZ05_002963"
     CDS             12365..14098
                     /gene="argS"
                     /locus_tag="JNZ05_002963"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAY8566125.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRSLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            14275..>14559
                     /locus_tag="JNZ05_002964"
     CDS             14275..>14559
                     /locus_tag="JNZ05_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HAY8566126.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDTQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLK"
BASE COUNT         3718 a         3532 c         3270 g         4039 t
ORIGIN      
        1 cttgggtaat tcgtagctgg tgggggtgtc tgtgtagctg taggagttgt atctgttacg
       61 ttgtctcgat gcctgaagca tcgctctaaa ttctctgtcg ttcattcata agctagtcct
      121 tataattgtt aattctctgt taacttatat agacaatact agcattttca aagactaatg
      181 tcaagcattt tttaacattt cattaacatc tgtggtataa tggttctatg gggagagtaa
      241 aatataaagg cgtggctatg agagtggtca tatggttatt gcattgcaca cagacctctt
      301 caatcgctct gtaacgagtt aaatggcctc ttcaggtcaa catataaggt caggtacttt
      361 tttgtgtcag aatgcgattg agagcgtctt acaaggaaaa ctaatggtaa atctccctcc
      421 tgagctgggg tcacctgatg tatgggctgg tggaggtgtt gggctgattg caggtctgct
      481 acttcgctac gtcctcgtca gagtgcttgt tgtctttaaa ctgttcggtc gtacatggac
      541 gcttgacctt aaggataacg accacacaga accccgttaa actcccttca aaaccccctg
      601 tacgtgatgg tataggggta tctttagcat tttgtgctat ctattaataa atagcttgac
      661 ataaccggaa aactgaatct atcaagtatt cacccctctg acagtaattt ctaaaaatgc
      721 ttatttttca atgagttgta attagccaat ataaaggact gacaagactc ttcacagaga
      781 actttcaaga ctcactaaga cacaaggcga taaccccaga cagggagagc ctccttctta
      841 accgacttct taaacaatct ttacagaccc ccgttcacca gtctcaatat ttttatttct
      901 cgctcttcgt tttttgctcg tttctattat tgagggggtt tgaaaaacct ctgtaaaata
      961 acccttccag taaaaagcga ataaccccag aaaggggaaa tcgcatcttt aacattctcg
     1021 taatcagtgc tctatatgag aacttcagcc caacctttga acaatcctgt aagtggtgct
     1081 tagaggtatt cttagcgatt ctgcgatgct cttgatgggt tgcccttggt catacatttt
     1141 aatcaacctg tcaagcgtct gtgcatcgtg catatgaggt cttccacgta aaccacccct
     1201 gttaaccacg gcaagtaaaa gcattgtgtc accacaatag attgtctggg taacttcaac
     1261 tttcggtttg cggggtgtgt tcctgtatat caaaacgcaa cttggaaacg gtgagcgcac
     1321 tacaacacct ctctttcctg ctggtggaag gtatttgact gctcggtgta acgttcgaat
     1381 ctcatgcagg aaggggtttg tatatacaca ccgaagccag taagttgtct ttgacctgag
     1441 cggaaccaga catacacaca catcagcttc aggggctttt aagaggaact tttcagccat
     1501 gctaaacggc gggttaacga agacccgttc accaacccat gattgattgt gaatatcatc
     1561 ggtgaaccgt ggcaacaatg cgtcatactc agttgcagca ccatctagac tgaagttaaa
     1621 ctcacgatgc aggttatgaa ataaacgata cggtgtacgc cagtcatcag aaccaccatg
     1681 ataacgacca acattgatta ttggatgcga cacaagcgaa gcgcccctct tagtatattt
     1741 cttaatcgtc tctttagcat ttagtgcttt ttgttaagtt tagcactttt tgctaaagac
     1801 aaaagcattg acaaaatggt aggattgttc acatagccct tacttaacag actcttaaga
     1861 gctttgaaac agcctgttta acaaggtcac tacctctggt cttatcgttc ccttctttct
     1921 aggtagtcat ctgttaagaa cttttcagtt ttattcttaa gataattgtt aagatgatac
     1981 tctcccttcg ggttatatcg tatctctcct cccgttaaat atcccttcac agtcagacta
     2041 aataacacct gtgtagtttc ttggtagaat ctttcaaggt aacttaactc tgagtttatg
     2101 aagttgacct atgagataga ctctaccgaa tctgttagtc aattgttgct taggtgctgc
     2161 taaggcttac ttagtgggaa gagagggaat atgtgaccat aggaacccct cctgttaaaa
     2221 gcttcttgac gttactatct cgatatgtat aatggcttac tgtactaggt ttacttccaa
     2281 agcaaccaag cacactctct atggcagtgt ccagcgaggt aaggtcgtgt agggtgcggt
     2341 tcggtggggt aaggtttggt gcggtatggg tggattgtcg tagccacgtt aaaaatcgac
     2401 ttatagggac tctttcggga gtccttttta gtttgtataa aatctgtaca gtaacttttc
     2461 agagtctgaa aatttttata aatatcgtag taacccctct acattcccct tcaccgataa
     2521 ttttcaaaaa tatctacact caattgatgg ctgttaagta ccccagattc acgatatagc
     2581 cccccccctt tgcaaaagca gttaaaatat ctataaccaa ccattgactt tcttcatgat
     2641 atctgtttag ccccacctca ataagccatc atcacacatc aaagcatcta tcaagaaggt
     2701 ttaacctatc aatagctgta acattttata gttctgtgaa gtcctatcca gttttctctg
     2761 taacagctac aaacttctgt gaccttatgt gtcaatgact tataagacat gctctcaagg
     2821 tgctgtatag cctcttatca gccaatacac aaaaccctta taaatcagta ctgatatgta
     2881 tacagtatgt ttttaacata atagctgttt tacgcactaa taagcaccta cgtagattct
     2941 gtatagagat taagatgtct ctaagtgatt ttttaagagg agatacgaag gaactataac
     3001 catcacttca taactttcac gtaggtaact attaagtagg ttgatagtca ctttgaggga
     3061 atattgatag tgaccaactg catagacacc gttatatagc cctgtatata gcactatatg
     3121 tagtgtctgg agagggacga ctaacacgat atagtttacc ctgaaaagag gatttacaga
     3181 ttctctatac atttcaataa gttatgataa aaaggggcta tgtcgatact gtatagaatc
     3241 tgtgtaggat tgcatagata cttggaaaaa taccgttaaa ttgcataggt actaacctgt
     3301 ttatgtcctg ttatttacag gcatagcatt attggttaat taattcatcc gactctatat
     3361 cattaccttt ttctttttcc tttttaattt tagcctctac catatcgaac attttcattg
     3421 tctgtgatga agttagctct ttcttttgtc gtagcgcgtt acgtatggta gaagcttcaa
     3481 tatcagaaac tgttaaaagt tcttctaccc tactcttcaa ttgctgtaat gaacggctat
     3541 tatcaacttt aaagaaatca tccattacct caagtgaggc aacaatcaaa tcctgtagtc
     3601 tctctttttc tatctcgata ttttcaatgg tttgtttttc tttagatact aactgcttta
     3661 aattattaat ttctttctga atcagtttct tctctgtctg tagctcttct gtgaccagct
     3721 cttcacgctt accacgctct acttccgctt taatatcagc atcaatttta gcattttctt
     3781 tctcttcttc tctggcagcc cgaaccttta accccgttaa cctgattgaa tccaagtatt
     3841 ctttttcctt tgtaagataa acattttcat tgtgtttatc tgtagcccat ttttgagcaa
     3901 gagaaagtaa ccattgagca taaggagtaa taacagctag cacacctcca aaaaagacag
     3961 gagcaagcag ataatgagcg taaaaatatt cctgagatag tatataatct attcgaaact
     4021 taacaggcgc atcactcata aagagcattg ctaagttagg ccagttgaac catacccatg
     4081 caagaatgat atatccccat aatgggcttt taatcctatc gtttattgct tgcatagtag
     4141 aatctaacca cccatcactc atagctaacc ctcggaagtc ttatctttct gtctatcata
     4201 accacaagca tcacctttag gaaccatctg aaacgggagg tgtatttaga ccttgctaat
     4261 cttagacagc cgcccggttc atgcatcacg tccacacagt gaagctattc gcgacgctca
     4321 acgaaaaaaa cctaaagttc cggtacatgc tgtactgacg gcgacaaatc cgctgatccg
     4381 ttttatcggt agtgatgata tgacgcaaaa ccgggaatta tttcaggtct ggttacaaaa
     4441 attagcgcag tggcatcaga ccactacgcc ttatcttttt ttacatacgc cagatattgc
     4501 ccaggccccg gaactggtac ataccctgtg ggaagactta cgtaaaacgc ttccagagat
     4561 cggagcagtt ccggctattc cacagcaatc ttctcttttc tgaatttgcc acctatcata
     4621 gacaggtgcc atcggccatt ttaaagggag tttgtatggt aagcgcgcta tatgccgttt
     4681 taagtgcgtt gttattaatg aagttctctt ttgatgtcgt tcgcctgcga atgcagtacc
     4741 gcgttgccta tggtgacggt ggttttagcg aactgcaaag cgctattcgc attcatggta
     4801 acgcgatgga atatattccc atcgcgattg tgctgatgct gtttatggaa atgaatggcg
     4861 cagaaacctg gatggtgcat atttgcggca tcgttttgct tgctggtcgt ctgatgcatt
     4921 attacggttt tcatcaccgt ctgtttcgct ggcgacgttc cggcatgagc gccacctggt
     4981 gtgcgctgtt gctgatggtg ctggcgaatc tttggtatat gccctgggag ttggttttct
     5041 ccctgcgtta gcgcacaata cgccactttc tttttcccgg atttttacgt tatgtctcac
     5101 cgcgacacgc tattttctgc ccctatcgcc agactgggcg actggacctt tgatgaacgg
     5161 gtagctgaag tcttcccgga tatgatccag cgttccgttc ccggctattc caatattatt
     5221 tccatgattg gtatgttagc cgagcgcttc gttcaacctg gtacgcaggt ttacgatctg
     5281 ggttgttctc tgggcgcggc gacgctctcg gtgcgtcgca acattcatca tgataattgc
     5341 aaaattattg ccatcgacaa ctccccggcg atgattgaac gctgccgtcg tcatattaac
     5401 gcctataaag cccctacgcc agtagacgtc attgaaggtg atattcgcga tatcgccatt
     5461 gaaaacgcat cgatggtggt gctgaatttt accctgcaat tcctggaacc ttccgagcgc
     5521 caggcattac tggataaaat ttatcaagga ctgaacccgg gcagcgcgct ggtgctttcg
     5581 gaaaaattca gtttcgaaga tgccaaagtt ggtgaactgt tgttcaacat gcaccacgac
     5641 tttaaacgtg ccaacggtta cagcgaactg gagatcagcc agaagcgcag catgctggaa
     5701 aacgtgatgc tgaccgattc cgtggaaacc cataaagcac gcctgcataa agccggtttt
     5761 gagcatagcg agctgtggtt ccagtgcttt aactttggtt cactggtggc attaaaagca
     5821 gaggacgctg catgatcgac tttggtaact tttattctct gattgccaaa aatcatcttt
     5881 cacactggct cgaaacgctg cccgcgcaga ttgctaactg gcagcgcgag cagcagcacg
     5941 ggctgtttaa gcagtggtcc aacacggtgg aatttctgcc tgaaattaaa ccgtatcgtc
     6001 tggatttatt gcatagcgta accgccgaaa gcgaagagcc actgagcacc gggcaaatta
     6061 aacgcattga aacgcttatg cggaacctga tgccatggcg caaagggccg ttctcactgt
     6121 atggcgtcaa catcgatacc gaatggcgtt ccgactggaa atgggatcgt gttctgcccc
     6181 atctttctga tttaaccgga cgcaccattc tggatgtcgg ctgcggcagc ggttatcaca
     6241 tgtggcgcat gattggcgca ggcgcgcacc tcgcggtggg gatcgaccct acgcaactgt
     6301 ttctgtgcca gtttgaagcg gtgcgtaaac tactgggtaa cgatcagcgc gcacatttgt
     6361 taccgttagg tattgaacag cttccggcac tgaaagcctt tgataccgtc ttttcgatgg
     6421 gcgtgcttta ccatcgccgt tcgccgctgg agcatctctg gcagttgaaa gatcaactgg
     6481 tgaatgaggg tgaactggtg ctggaaacgc tggttattga tggcgatgag aacacggtgc
     6541 tggtaccagg cgatcgttac gcgcaaatgc gtaatgtcta tttcatccct tccgcgctgg
     6601 cgctgaaaaa ctggctgaag aagtgtggtt ttgtagatat ccgcattgtg gatgtgtgcg
     6661 ttaccactac tgaagagcag cgacgcactg aatggatggt taccgaatct ctgtccgatt
     6721 tcctcgaccc acatgatccg agtaagacag ttgaaggtta tcctgcgcct aaacgcgcgg
     6781 tgctgattgc gcgcaagccg taaaggtctg gtattactgc cggatgcggc gtgaacgcct
     6841 tatccggcct acaaaccctt gctaattcaa tctattgcag ggactatgta ggcctgataa
     6901 gcatagcgca tcaggcagtt ttacgtttgc ataacctcag cgcccgtttc cgggcgctat
     6961 tcacttctta cgcctgaaca gcaggcttat caaatgccgt taacttcggt gcgttacccg
     7021 tatatttttc gatatacacc agcgcagagt taccggcaca ggcatttgcc agctgcgagc
     7081 tggggatatc cgccgttaac acgttcgcac tgccgttttt acacaagccg ttttccagat
     7141 ctggccatgc accttcatgc acgcatacca cgcctttttt gatcccgtca gtcaccaccg
     7201 cgcctgtcaa aatctgacca cgtttgttcc agacacgcac cagatcgcca ttcgcaatac
     7261 caaatcgagc agcatcttcg gtgtgaatag tgatcggttc acgatcggca accgcatatt
     7321 ttttacgcag ttccgcatag ttaagctgac tgtgtaaacg gtgtgccgga tgtgcggtca
     7381 gaagctgcaa ctgcttctcg tcggcggtac ccttccactc atcaggcgca agccaggttg
     7441 ggtgtgccgg gcaatcctta tagccaaatt tttccagcgt tttggagtaa atctcaattt
     7501 tgccgcttgg cgtacccagc gcatttttca ccggatcggc gcggaaatca ccataacgaa
     7561 cgtactgttc gttcttctcg ctgcggcgca tttcgatcag tttattttgc tgccagaagg
     7621 cattaaacat tggcatagtg acgcgttgcg cacgggcccc tttctgagcg gcatcataga
     7681 aaaatttcag ccacgccatt tcatctttac cttcggtata gatctctttt ccgccaggtt
     7741 tgagtaattc cgcaagatcg gcaaacacgt caaaatcgtt acgcgcttca aattgcggag
     7801 caacagcctg cttcatcggc acaatatgct ggttgctgta atcaccggtc atcgtcaagt
     7861 cattgcgctc aaacgatgtg gtgatcggta atacgatatc tgcgtgtttg gctgctgcgg
     7921 tccagtagca ttcagaaacg acgatcatct ccggtttctg ccaggctttg atcagacgat
     7981 tagtatcctg gtgatgggta aagttaccgc cacccgccca ccagatcatt ttgatattcg
     8041 gatacgtttg ttctttaccg ttatgctgat atttgccgcc aggattttcc agcgcatcga
     8101 cgatacgggc aacaggaatc gccgtcattc cgccatcatc agcggcctcg ctggcctgtc
     8161 cggcaatcgc cgcagacatt tccggcagca cgccgccaac acgcatcggg ttgccaccat
     8221 tggagtaatg ataagagaaa ccaaaaccgc cgcccggtgt accaatctgc cctaacatcg
     8281 ccgccatcag ttcagcgagt ttgactatct gcgcttccgg tacgccagta atttccgccg
     8341 cccagaccgc gctcttcggc gtattatcgc tcttacctgt cagatactct tcaaactgcg
     8401 gatacccggt agtgtatttt tcaaggaaca ctttatcgtg tttgccttgt gtcatcaggg
     8461 tatgcgcaat ccctaacatc agtgccacgt cggtgcccat attcggcgcg atccaggtgg
     8521 cattatcgcc aaagaattcg atggtttcgg agcggatagg atcaatggca atcactggtt
     8581 tgccagattt tttcagctga tggaagtatt ccagcccttg ctcatcggta ctgctccagg
     8641 caatttttaa ggtattcagc gggttcattc cccacagcac cacaacctgg ctgttttcca
     8701 gaatcagcgg ccaggaggcc tgctgttcat acacctctac agaaccgacc acatgcggca
     8761 tgatcacctg tgctgccccg gttgaataat cgccgctatg cccggaataa ccgcccgcca
     8821 ggttcatata acgttgcagt aaggtttgcg ctttatgcaa cacgccagaa gagcgccagc
     8881 cgtaagatcc ggcaaaaatg gccgatggtc cgttagcttt acgaatacgc tcatgttgct
     8941 catgaatcag ttttaatgcc tgttcccagc tcacctgtac ataggtatct tcgccacgac
     9001 ctttcgccgg ttgcagtgga ttgtcgagat agctttttct caccatcgga tgctgaatac
     9061 gcgccgtggt gtgtacctga tccgccgccg tagactgtaa ggagttcggt atggttttcg
     9121 ccagcgcgcc tgtcgaagaa acaatcttgc cgtccttcac ttctacgctc atcgctcccc
     9181 aacgtcccgc ggtgaggatt ttaccgccct tctcttctgc ccatgcgggg agcggtgctg
     9241 ccgatgtcac caccagcgct ccagcggcaa taccgctgtg tttaataaat tcacgtattg
     9301 ttaatgccat aacttcctcc ctgatcaacg aggatcactg tttctcggta atatctttgg
     9361 cgttgtactg gaaataccgc gttaaaatgt ccagttcgtt ttcgctcatg ctggttcgtg
     9421 cccccattcc tttggcaatg gacggccacg cattgacggt gtaatggtcg gcggcaatag
     9481 aggcatgaca accagcgcaa taggtatcgt caagtttttc agcgtattgc catagcggtt
     9541 tacggtccgc taatgcggga tcggtaagcg caccctgtaa agacgcctga cgccattgat
     9601 tgccgtattc gtcagcctgc cattccccgt ttaccgtgag cgccttgata ccttcttcac
     9661 ttaatgtggc tagcgccagc cgttgacctg ccgccaggta gagcgtgttt tcactgccct
     9721 gcatttgata accctgcaac agaacgatcg gctgtttgcc actggcatca acgacggtga
     9781 gatcggtacc aggattcacg gtagccagct cgcctatgcg agaagttttg aaaggataaa
     9841 tatgtgcgcc attagtcact gaagtagcgg cctgactttc cagctcatgc gccgcgttgt
     9901 catccatttt tatttctggc ggaaaatggg caatgccttt atgacaatcg atacaggttt
     9961 cgccgccctt ttgtgctttg ttatgcattt tctgcgcaga ttcactttgc gaggcaatat
    10021 ccatggcatc aaaagaatgg caactacggc acgttgcaga gtcagttgct tttaattctt
    10081 tccatactgt ttcggccatt tcctggcgat gagtttcgaa cttatcgtca ctgtctattt
    10141 tgccgctaac aaattcatga taaatatctt tagatgcttt taatttagca aataaataat
    10201 ccatccctga ctttggaata tggcaatcgg cacattccgc acgtatccct ttctggttcg
    10261 aaaagtggac agttccctga tattcctcaa aaggtttact catcgagtgg caggaaaggc
    10321 aaaatgctgt atccgacgtt ttatgtaagg ctttttgcgc cagcaataac ccgccgccac
    10381 caaccacaac cgctatcagc aaaaacaata acccaatgcg ttttttccct cgcatagatt
    10441 cctctaaata aattccagaa caccgaaatc attgtataat taaatacaca acgaattaac
    10501 cgcttgcata actcggcaca acactgcctg aaataatcga taaagaatat gatttattac
    10561 aatgtaatca ttaattgcta aggaataacc cagttgctat ttagaatata tatttatcag
    10621 ttttagcaat ttaaatccca taatcaatgt gaatataaaa aatattttta gcacaacgct
    10681 atgcgcactg tcacccgctc aacagatcac ggttatcgtt cgttttttat actgctcagg
    10741 gataaaaaaa ggcccctgtt gaaattgcag gggcctggta cgagcaagca tcatattggg
    10801 cgacatgatg caacggtaaa aatcatttgg cctgatggcg ttcaatgatt cctttcattt
    10861 cagcaaccgc cgccccgtct acgatataac gcgaatactc gtccgcgtgt tcatctgatg
    10921 acatggacaa tccttgatta cgataacgca tcggtgaggc ttgccacgct cccgcggagc
    10981 tatggacttc cagcactccg gcatcgagga agtggtgcag gttttctgca cggactcctg
    11041 ctccagccat aatgattgga gcatcacgat gggcaataag ttccataatt tttgataaac
    11101 cttgcagtgc gtctgatttt tgccctgatg tcagtacccg ggcaatgccc aattccgcaa
    11161 gattattgag tgtatataaa gggttagcgc acatatcgaa ggcgcgatga aaagtcactg
    11221 ccagcggacc ggcagcagcc attatttttt ccattcgtgg catatcgaca ttcccatcaa
    11281 catcgagaac gcccgtcacc agtccaggaa aacctaattc gcggaccgtg cgcacatcct
    11341 caagaatggc ggcaaactca ccgtcgctgt agcaaaaatc accaccgcgt gggcgaatta
    11401 tcggatgcac agggatcgtc acccgctggc gcacggattt cagtcccccc aacgacggcg
    11461 ttaagccccc ctcttttggg gctgcgcata attcaactct gtctgcgccg ttttgctgcg
    11521 ccgttagtgc acattccatg ctgtaacagc aaatttccag taatgccatt tttgctcctt
    11581 aattacgctg actgctcgct ggcaacgatc tcttcaatgg accacggatg aaacttaata
    11641 gttgttttgc catcggtgac cgccagcgtc ggattcggta atcgctcatg ctcacctttt
    11701 ggtgaactgg ttttaactga gatccccggt aggctgagtc cttcatcaga aagcaatgcc
    11761 agcgcacggg cattcagcgt ttctggatca cccggcagaa cgatttcaat atgttcccaa
    11821 ccttcgtgtg ggtaacgttt ttccccaggc cacggtagct ccacattgga aaactgccag
    11881 tgcgcaacct gtaccggttc atgcaattta aacagacaaa tcggtctgcc attgatcatt
    11941 ttttctgaca aaagctcgcc acactgttca aaaccgcgac gccagcgctc agcggtgacg
    12001 ttttgatggc aacgcaaaga aatgtgatcg gcagtcagcg gagtgatatc caaccccaga
    12061 cggcgggaaa gttcatctaa tgcgtgggta aatcgcggta aatccgatgc aatatcctgc
    12121 agctcgtcga tagattgcca gttcgccata atcactcttc gtctttcagt aaaagcgtta
    12181 atttaccctg ttgccctgtg ccaaccaacc gctgatttca cgccgcttct gatgcaatag
    12241 tgaaaacggc aatacgccac gcgcacgttg ctgacgaaaa cagccatttg cagtatactc
    12301 ccaccctaat ttctttaact ggtgcgggca atttttgctc gcttcatcaa tgtaaggtat
    12361 tccggtgaat attcaggctc ttctctcaga aaaagtccgt caggccatga ttgcggcagg
    12421 cgcgcctgcg gattgcgaac cgcagattcg tcagtcagca aaagttcagt tcggcgacta
    12481 tcaggctaac ggcatgatgg cagttgctaa aaaactgggt atggcaccgc gacaattagc
    12541 agagcaggtg ctgactcatc tggatcttaa cggtatcgcc agcaaagttg agatcgccgg
    12601 tccaggcttt atcaacattt tccttgatcc ggcattcctg gctgaacatg ttcagcaggc
    12661 gctggcgtcc gatcgtctcg gtgttgctac gccagaaaaa cagaccattg tggttgacta
    12721 ctctgcgcca aacgtggcga aagagatgca cgtcggtcac ctgcgctcta ccattattgg
    12781 tgacgcagca gtgcgtagtc tggagttcct cggtcacaaa gtgattcgcg caaaccacgt
    12841 cggcgactgg ggcactcagt tcggtatgct gattgcatgg ctggaaaagc agcagcagga
    12901 aaacgccggt gaaatggagc tggctgacct tgaaggtttc taccgcgatg cgaaaaagca
    12961 ttacgacgaa gatgaagagt ttgccgagcg tgcacgtaac tacgtggtaa aactgcaaag
    13021 cggtgacgaa tatttccgcg agatgtggcg caaactggtc gacatcacca tgacgcagaa
    13081 ccaaatcact tacgatcgtc tcaacgtgac gctgacccgt gatgacgtga tgggcgaaag
    13141 cctctacaac ccaatgctgc caggaattgt ggcggatctc aaagccaaag gtctggcagt
    13201 agaaagcgaa ggggcgaccg tcgtattcct tgatgagttt aaaaacaagg aaggcgaacc
    13261 gatgggcgtg atcattcaga agaaagatgg cggctatctc tacaccacca ctgatatcgc
    13321 ctgtgcgaaa tatcgttatg aaacactgca tgccgatcgc gtgctgtatt acatcgactc
    13381 ccgtcagcat caacacctga tgcaggcatg ggcgatcgtc cgtaaagcag gctatgtacc
    13441 ggaatccgta ccgctggaac accacatgtt cggcatgatg ctgggtaaag acggcaaacc
    13501 gttcaaaacc cgcgcgggtg gtacagtgaa actggcagac ctgctggatg aagccctgga
    13561 gcgtgcacgt cgtctggtgg cagaaaagaa cccggatatg ccagctgacg agctggaaaa
    13621 actggctaac gcggttggta ttggtgcggt gaaatatgcg gatctctcca aaaaccgcac
    13681 cacggactac atcttcgact gggacaacat gctggcgttt gagggtaata ccgcgccata
    13741 catgcagtat gcttacacgc gtgtattgtc cgtgttccgt aaagcagaaa ttaacgaaga
    13801 gcaactggct gcagctccgg taatcatccg tgaagatcgt gaagcgcaac tggcagctcg
    13861 cctgctgcag tttgaagaaa ccctcaccgt ggttgcccgt gaaggcacgc cgcatgtaat
    13921 gtgtgcttac ctgtacgatc tggccggtct gttctctggc ttctacgagc actgcccgat
    13981 cctcagcgca gaaaacgaag aagtgcgtaa cagccgtctg aaactggcac aactgacagc
    14041 gaagacgctg aagctgggtc tggatacgct gggtattgag actgtagagc gtatgtaatc
    14101 gatttttcgt gagagtgaag cctgatcagg gttagccgat caggcttttt tattgccatc
    14161 taaatgtatt ctgaaaacgg acatgccatt gttttctcac tgttgagtaa gaggccagaa
    14221 gcgtaatatc caggcccagg aaaacgataa ctgttcaatt taaggaatac cgaagtgttt
    14281 aaatttcttg tattaacatt aggcattatc tcttgccagg cttacgcaga agatacggtt
    14341 atagtaaacg accatgacac ttcagccatc aaagattgtt ggcaaaaaaa ttcagatgat
    14401 gatactgaca ttaacgtgat caaatcatgc ctgcgacaag aatacaatct cgtcgatacg
    14461 caattaaata aagcctatgg tgaagcttat cgttatatag aacaagtgcc acgcacaggt
    14521 gcaaaaaaac ttgatactga acaacttaac ttgcttaaa
//
LOCUS       DADMEQ010000097        14527 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.098, whole genome shotgun sequence.
ACCESSION   DADMEQ010000097 DADMEQ010000000
VERSION     DADMEQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.098"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(337..627)
                     /locus_tag="JNZ05_002965"
     CDS             complement(337..627)
                     /locus_tag="JNZ05_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566127.1"
                     /translation="MDATGNTTVTCLSHKMKYKEVACGKQRMHWLALQFVDENNNPVS
                     GLNVQLEYHPRAMAAELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1227..1302)
                     /locus_tag="JNZ05_002966"
     tRNA            complement(1227..1302)
                     /locus_tag="JNZ05_002966"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Phe,seq:gaa)
     gene            complement(1408..2115)
                     /locus_tag="JNZ05_002967"
     CDS             complement(1408..2115)
                     /locus_tag="JNZ05_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAY8566128.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAIPLSAAWTAWFA"
     gene            2513..4648
                     /locus_tag="JNZ05_002968"
     CDS             2513..4648
                     /locus_tag="JNZ05_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY8566129.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4697..5953)
                     /locus_tag="JNZ05_002969"
     CDS             complement(4697..5953)
                     /locus_tag="JNZ05_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HAY8566130.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGSELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGTFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="JNZ05_002970"
     CDS             complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="JNZ05_002970"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HAY8566131.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7299..7574)
                     /locus_tag="JNZ05_002971"
     CDS             complement(7299..7574)
                     /locus_tag="JNZ05_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HAY8566132.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="JNZ05_002972"
     CDS             complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="JNZ05_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAY8566133.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAPLPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8815..9534
                     /gene="trmB"
                     /locus_tag="JNZ05_002973"
     CDS             8815..9534
                     /gene="trmB"
                     /locus_tag="JNZ05_002973"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAY8566134.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9534..9860
                     /locus_tag="JNZ05_002974"
     CDS             9534..9860
                     /locus_tag="JNZ05_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF469 domain-containing protein"
                     /protein_id="HAY8566135.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10044..10763
                     /locus_tag="JNZ05_002975"
     CDS             10044..10763
                     /locus_tag="JNZ05_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HAY8566136.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10939..11985
                     /gene="ansB"
                     /locus_tag="JNZ05_002976"
     CDS             10939..11985
                     /gene="ansB"
                     /locus_tag="JNZ05_002976"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HAY8566137.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12102..13109
                     /locus_tag="JNZ05_002977"
     CDS             12102..13109
                     /locus_tag="JNZ05_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HAY8566138.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13165..14466)
                     /locus_tag="JNZ05_002978"
     CDS             complement(13165..14466)
                     /locus_tag="JNZ05_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAY8566139.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTLPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3807 a         3476 c         3735 g         3509 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgtagg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct acttagaaga aatgttcagg ctggatggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gcccggactc ggaatcgaac caaggacacg
     1261 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1321 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1381 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1441 cggtattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1501 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1561 cgacggtgtg gtcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1621 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1681 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1741 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     1801 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     1861 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     1921 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     1981 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2041 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2101 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2161 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2221 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2281 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2341 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2401 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2461 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2521 aatgaatatt gccgccagta gtgaactggt atcccgactt tcttctcatc gccgcgtggt
     2581 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2641 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2701 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     2761 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     2821 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     2881 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     2941 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3001 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3061 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3121 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3181 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3241 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3301 cgtggcgcca gaaaaagccg acctgccgcg cccgtttcgc ctggcgatta ttcagctggg
     3361 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctctg
     3421 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc
     3481 ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3541 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3601 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3661 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3721 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     3781 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     3841 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     3901 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     3961 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4021 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4081 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4141 gctggcacaa ctggtggcga tgctggcgca atttgaacag catattgagg atgactcgtc
     4201 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4261 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4321 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4381 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4441 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4501 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4561 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4621 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4681 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4741 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     4801 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     4861 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac gtcccttgtg
     4921 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     4981 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5041 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5101 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5161 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5221 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5281 gcagttccga gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5341 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5401 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5461 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5521 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5581 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5641 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5701 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     5761 atttgtcggc cacaatcccc agtagcgcag gcataaatac cgcggcgata cccagtgagc
     5821 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     5881 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     5941 gcttaagatt catgttaatt tcctcacatc gtgattcgga tgagtgtttg aaaaagcaca
     6001 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6061 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6121 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6181 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6241 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6301 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     6361 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     6421 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     6481 atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc
     6541 ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg
     6601 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc atgccgagat
     6661 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga
     6721 tacgtagtcc gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc
     6781 ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     6841 aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     6901 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     6961 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     7021 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc
     7081 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     7141 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     7201 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     7261 tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     7321 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     7381 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     7441 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     7501 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     7561 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     7621 cgtaactgct gtaacaaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     7681 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     7741 cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtcagttgc
     7801 gtcaggttat cggcagcaat ctgccgttgt gccagccaat gccgcaaact ttcttcgtcg
     7861 gcaaactgcg ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc
     7921 aatacttcat cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcgtctgt
     7981 ttcggttttt tgcccggata aagcgaccag ctattgttgg tggcggcaat acatccgttt
     8041 tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc gcccaaatcc
     8101 atcatcgcct gattaaaccg ttccacgccg accgcgggcg tcacctgctc gctcaaactc
     8161 catagtttat tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc
     8221 agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct tgcccagaga aagcgagaga
     8281 atcgcgcctg cggtggaacg cccgacgccc ggtaacggcg cgacttcttc aaaggtttcc
     8341 gggaatttac caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg
     8401 cgggcgtaat agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg
     8461 agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa aataggggat aacggtcgca
     8521 acctgagttt gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt
     8581 tgccagggca gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat
     8641 tgcgacgctt gcatggtcac cgacttcact gttgttgggg gcaagattgc agcacagcga
     8701 cggcagggtg taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa
     8761 ctaactttgg ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa
     8821 aacgacgtca tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt
     8881 tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaattactgg
     8941 ccggtgatgg gcgttgagtt cagtgaagat atgctggatt tccccgcgct ttttggccgt
     9001 gaagcgccgg tgacgcttga gattggtttt ggcatggggg cgtcgctggt ggcaatggct
     9061 aaagatcgcc ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg
     9121 tgcctggctt ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg tcacgatgcg
     9181 gttgaagtgc tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc
     9241 cctgacccgt ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc
     9301 gaactggtaa aaagcaaact gcagctgggg ggcgtattcc atatggcgac cgactgggaa
     9361 ccttatgcgg aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca
     9421 gagagcaatg attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt
     9481 ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa
     9541 agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat
     9601 tttcggtggc atggcgattc ccggaaggta catcggaaga acagattgat aaaatcgttg
     9661 atgactttat taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc
     9721 tggcctggga aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg
     9781 cgattgtgcg taagtggctg gaagagtgca aactggatga ggtacgcacc agcgaacttt
     9841 tcgacgtttg gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg
     9901 cgctacgctt atcaggccta cagagatgca cgatcgagta ggcgggataa ggcgtttacg
     9961 ccgcatccgg catggaaaac gcgtactttg ttatcaatct ggggccagca tatgctggcc
    10021 tgatttgttc ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag
    10081 tgacggcaat gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga
    10141 ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag
    10201 acggcaacgt gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg
    10261 cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa ggcgcgaaaa
    10321 gccgcgtcga gaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaca
    10381 gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca gcttaaagag cagatgaatc
    10441 gcattatcga aacgcgcagc gatggcctga cgttccacta taaagccatt gatcaggttc
    10501 gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg aattttacag gacagcatta
    10561 atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg
    10621 gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg gaaaaagcag gaaaaagatt
    10681 tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agccgcaaag
    10741 ccctggtcgg gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc
    10801 gtttaattct tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg
    10861 ttgtaaattg tttaacgtca aatttcccat acaaagctaa gggataatgc gtagcgttca
    10921 cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg
    10981 ggttttagtg gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc
    11041 attgccggtg gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta
    11101 gaaaatctgg ttaatgcggt gccgcaacta aaagacattg cgaacgttaa aggcgagcag
    11161 gtagtgaata tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa
    11221 attaacgccg actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg
    11281 gaagaaactg cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc
    11341 ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg
    11401 gtagtgaccg cagctgataa agcctccgct aatcgtggcg tgctggtggt gatgaacgac
    11461 accgtactgg acggtcgcga tgtcaccaaa accaacacca ccgacgtagc gaccttcaag
    11521 tctgttaact acggtcctct gggatacatt cacaacggta agattgacta ccaacgtacc
    11581 ccggcacgta agcacaccag cgatacgcca ttcgatgtct ctaagctgaa tgagctgccg
    11641 aaagtcggca tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta
    11701 gatgcgggct atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc
    11761 gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc
    11821 gtaccgacgg gtgctaccac tcaggatgct gaagtggatg atgcgaaata cggcttcgtc
    11881 gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa
    11941 accaaagatc cgcagcagat ccagcagatt ttcaatcagt actaatcacc tcgtcccggc
    12001 atcgtgccgg ggctttttca cttcagactt acgtccattg ccaattttca ttatcctaat
    12061 gataatcacc ggaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg
    12121 ttggcacggc gctgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa
    12181 ctgaaaatat cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg
    12241 ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc cacctggtca gcggaaggcg
    12301 atgtctcttc cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc
    12361 tcgaacagac ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag
    12421 gaacgcccgc gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc
    12481 gtgggcgggt ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag
    12541 atttcaatta tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc
    12601 ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag
    12661 cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta
    12721 aaaaaattca ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg
    12781 ttaaatatga caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag
    12841 agtgttatga agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac
    12901 agacttttct gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg
    12961 aaaaagggca atatccgtta acgatgaaag tttcagaaat aaacagtaaa aaagtcgcga
    13021 ttttgatgaa aattgacgat atcaaccaag ctaatgaacg ctggaaaaaa gataccgaac
    13081 aactgcggcg caatggggtg attaagtaac tcatgttaag tgcctttgcc ggataatata
    13141 aaaagttatc cggcaaatta ttttttacat tagccctagt agctgtggaa tgaacaagga
    13201 tatttgcgga atgtaagtga tcatcatcaa cgctactagc aatgcagcat aaaatggcag
    13261 caatggtttg attaaatgtt gaatcttcac acccgagata gaacaaccaa caaatagcgc
    13321 actacccacc ggcagtgtta acagcccaat acaaaggttg gccaccatca taataccgaa
    13381 atgtactggg tccattccca gctcttgtgc aatcggcaag aagatcggtg taaagattag
    13441 tacagccggt gtcatgtcca taaagatgcc gacaattaat aagacgatat taatcagcag
    13501 caatatgatc agtgggttat cagaaatccc catcaatgcg tcgctgatca tataaggaat
    13561 atcagcatta gtcattgccc aggacatgcc cacggaaaaa ccgatcaaca gtaatacaat
    13621 cgaagtggta acaaccgact ccagaatcag tttgggtaaa tggcgccatt ttacttcccg
    13681 gtaaatcagc accgagagaa taaatgtgta gacaacggca attgctgacg cttcagttgc
    13741 ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac accagaagca aacttggtaa
    13801 ggcatccaga aacgctttac aggcctgagc aaaggttgct ctttctgaaa gcgggtagcc
    13861 acggcgttta gcaataatgc cgcagactat cattaccgca agacccatta agatccctgg
    13921 caaatatccc gccataaaca gcgatgctac agaaacgccg ccagctgtca gggaaaacac
    13981 aattaacacg tttgagggcg gtatcagtaa tccagtaata caagatgaga cgtttacagc
    14041 ggtagaaaat gccggatcat aaccttcttt ggtctgaatg ggattcagtg tgccaccaac
    14101 ggcagcagct gccgcaacgg ctgaaccaga aatggaacca aacatcatat tcgccaggac
    14161 gttcacatga ccaagcgaac caggaacgcg gccaaccatt acctgggcga ggttaatcaa
    14221 acgtatcgca ataccgccgc tgttcatcag tgttccggca aagatgaaaa aggggattgc
    14281 caacagggca aagttatcca gtccattagc cagtcgttgc gaaacagtga ttgccgcgat
    14341 atcccaggga aacatcaaaa gcattgaagc tacagtggca ataccgattg agaacgaaat
    14401 gggcacacca ataaagacca gcacaaaaaa acagccaaac agtgtaatcg cgatataact
    14461 atccattaac tggcctcctg cgcaggttgt ttcagttgtt gaatgctttg attaataaaa
    14521 cacaagg
//
LOCUS       DADMEQ010000098        13988 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.099, whole genome shotgun sequence.
ACCESSION   DADMEQ010000098 DADMEQ010000000
VERSION     DADMEQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13988
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.099"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            1..454
                     /locus_tag="JNZ05_002979"
                     /pseudo
     CDS             1..454
                     /locus_tag="JNZ05_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            514..621
                     /locus_tag="JNZ05_002980"
     CDS             514..621
                     /locus_tag="JNZ05_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HAY8566140.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(602..1333)
                     /gene="yehW"
                     /locus_tag="JNZ05_002981"
     CDS             complement(602..1333)
                     /gene="yehW"
                     /locus_tag="JNZ05_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HAY8566141.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="JNZ05_002982"
                     /pseudo
     CDS             complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="JNZ05_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
     gene            complement(2257..3415)
                     /gene="yehY"
                     /locus_tag="JNZ05_002983"
                     /pseudo
     CDS             complement(2257..3415)
                     /gene="yehY"
                     /locus_tag="JNZ05_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
     gene            complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="JNZ05_002984"
     CDS             complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="JNZ05_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HAY8566142.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDAPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="JNZ05_002985"
     CDS             complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="JNZ05_002985"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HAY8566143.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MMVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7084..8799
                     /gene="dld"
                     /locus_tag="JNZ05_002986"
     CDS             7084..8799
                     /gene="dld"
                     /locus_tag="JNZ05_002986"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY8566144.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="JNZ05_002987"
     CDS             complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="JNZ05_002987"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HAY8566145.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9943..10530)
                     /locus_tag="JNZ05_002988"
     CDS             complement(9943..10530)
                     /locus_tag="JNZ05_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HAY8566146.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            10700..11278
                     /locus_tag="JNZ05_002989"
     CDS             10700..11278
                     /locus_tag="JNZ05_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8566147.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(11395..12156)
                     /locus_tag="JNZ05_002990"
                     /pseudo
     CDS             complement(11395..12156)
                     /locus_tag="JNZ05_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(12209..13735)
                     /gene="mdtQ"
                     /locus_tag="JNZ05_002991"
     CDS             complement(12209..13735)
                     /gene="mdtQ"
                     /locus_tag="JNZ05_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HAY8566148.1"
                     /translation="MLLMYCHAKLKNISQHTVISAHRFLPDYSPMNRDSFYPAIACFP
                     LLLMLAGCAPMHETRQALSQQTPAAQVDTALPTALKNGWPDSQWWLEYHDNQLTSLIN
                     NALQNAPDMQVAEQRIQLAEAQAKAVATQDGPQIDFSADMERQKMSAEGLMGPFALND
                     PAAGTTGPWYTNGTFGLTAGWHLDIWGKNRAEVTARLGTVKARAAEREQTRQLLAGSV
                     ARLYWEWQTQAALNTVLQQIEKEQNTIIATDRQLYQNGITSSVDGMETDINASKTRQQ
                     LNDVVGKMKIIEARLNALTNHQTKSLKLKPVALPKVASQLPDELGYSLLARRADLQAA
                     HWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTLPIFD
                     SGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIERDAL
                     RVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLDASIRLTGALGGGYKR
                     "
     gene            13869..13952
                     /gene="yohP"
                     /locus_tag="JNZ05_002992"
     CDS             13869..13952
                     /gene="yohP"
                     /locus_tag="JNZ05_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HAY8566149.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3333 a         3905 c         3527 g         3223 t
ORIGIN      
        1 ggcaatctac atagcctgcg aacgtggatc aaagagcgcg gtcaggatta ccccgcccag
       61 acactcacca cacatctgtt tattcctctg cgccgacggc ttcagtgcca acaaccgact
      121 ctccaggcgc tgctggcgat cctcgacggc gtactgatca actacatcgc catttgtctg
      181 gcttcggcac gtaaaaaaca gggtaaagat gcgctggtgg ttggctggaa tattcaggat
      241 accacccgtc tgtggctgga gggctggatt gccagtcaac aaggatggcg cattgatgtc
      301 ctcgctcgcc cactcgctca atcaactacg ccctgaacta ttcgaaggcc gtacattgct
      361 ggtgtggtgc ggtgaaaatc gaacctccgc ccaacagcag caactcacca gttggcaaga
      421 acaaggccat gatattttcc cactcggtat ttaatgattc gttaacaaat gcgctttact
      481 gtacaatcct ttcgttaaca taaggagtgc attatgcgca tagctaaaat tggggtcatc
      541 gccctgttcc tgtttatggc gttaggcgga attggtggcg tcatgctcgc aggttatacc
      601 tttattttgc gtgctggcta agcgcctgca ccagcctttc aaacaggcgg tctgcgatga
      661 tcgccgccag tgccaccagt aacgccccct ggatcacata cgcggtatta aatccgctaa
      721 gcccgatgat gatgggcgta cccagcgtac tggcccctac cgttgaggcg atcgtcgccg
      781 taccaatgtt gataatcacc gaagttcgca cgcccgccag aatcaccgga gccgccagcg
      841 gtagctcgac cttacgcagt cgctgacctc gactcattcc catacctttc gcaacttctg
      901 tcacgctggg atcaatcgct cccagcccgg caagtgtcac ctgcaggatg ggcagcacac
      961 cgtaaaggat caaggcgata atcgctggtt gcagaccaaa gccgatcacc ggaacggcaa
     1021 tcgccagcac tgcgacaggc ggaaaagtct gtccaacggc ggcaatagtt tccaccagtg
     1081 ggcgaaattc cgcgccccac gggcgagtaa cagcaattcc ggcacctgtg ccaatgatca
     1141 ccgcaaacaa actcgaaatt cccaccagcc agaaatgagc cagtgccaga gctgcaaaac
     1201 tttcttgctg ataaacgggt cgtggcagtt gtgggaacaa ggcagcaaac agcggctggc
     1261 tgtaaggcag ccagaaaatc agcgccacaa aaagagcaat gagccagaac agcggatcgc
     1321 gcaacatctt catacgctta cgcctccacc agcagatcct gaaaatgcag cgtgccgcaa
     1381 ggctggccct gcgtgttcac caccggcagc acctcgcatc cccgcgcgac aaacagggag
     1441 agcgcatcgc gtagcgtcat ctcttctgcc agtgcctcac cttctgcccg ttcttcgcga
     1501 cgcacgtaat ccgccacgct acgtaacgaa agcaggtgcc cccccagttc actacgtcca
     1561 aaaaactggc ggacaaaatc attcgccgga cgagtcagca tcgtcagcgg attcccctgc
     1621 tgcaccactt caccgtgatc catcaatacc agatgttctg ccagccgtag cgcctcatca
     1681 atatcatgag tgaccagcac aatagtgcgc cccagcaaac ggtgaatgcg cgtcatctct
     1741 tgttgcaacg cgccgcgcgt taccgggtcc agtgcgccaa aaggttcatc catcagtaag
     1801 acttgcggat cggcagccag tgcacgcgcc actcccacac gttgctactg accaccggaa
     1861 agctgatgcg gataacgctc acgcaaattt gactccagcc ccagtagcgc cattaattcg
     1921 tcgatacgat cgtcaatccg cgcccgcgac catttttgta attgcggcac ggtagcgatg
     1981 ttttgcgcca cgctccagtg ggggaacagg ccaatagatt gaatggcata gcccatccgg
     2041 cggcgcaact ccagtactgg cagcgagcga atttcttctc cggcaaagcg gatcactccg
     2101 ctgtcatgct ccaccaggcg gttaatcatt ttcagggtgg tggatttgcc ggagccagat
     2161 gtgccaatca gcaccgaaaa actcccttcc tgaaaattga gattgagatc gttaacggct
     2221 ttttgtgcgc cgaacagttt gctgacatgg ctaaattcaa tcattacgtt tcaccttcag
     2281 cagtgcgata agcaaatcga acagcgcgtc ggtaagcacc gccagaacaa ttaccgggat
     2341 cacccccagc aacactaaat caatggcgct gcttagcagc ccctggaaaa ccagcgcacc
     2401 aaaaccgcct gcgccgatta acgccgcaat caccgccata cctacagttt gcaccatcac
     2461 cacccgcagg ctgcgcagaa ataccggtaa cgccagcggt aactgaacat gcaggaaacg
     2521 ctgcgccccg ctcatcccca tcgctctggc gctctccagc acatcgcgcg ggatctggtt
     2581 caaaccgact accacgccgc gcaccagcgg cagcaaggca tagagcacca gcgcaatcag
     2641 tgcgggtgtc attccggttc ctgctatgcc gagcttcccc agccacggaa aggccgtaac
     2701 cagcgcggca agcggcgcaa tcaacaggcc aaagagcgcc accgaaggca cggtctgaat
     2761 gacattgagc agagaaaaaa ttgccccctg ccgcgcagtg gaaaagtagc accagatgcc
     2821 caacggcaca ccaatcacta acgcaggcag caccgcacca aacagcaacg tcagatgttg
     2881 tgccagcgcg tcgtcaaaca catcctgacg gttggcgtat tctttcattt agtgagagat
     2941 cgttaagcgt gccggagtac agcaaccaca gcggaataat ggcaatctgc atatgcaaca
     3001 accagcgcca cagcggatgc gtggagattc ggcggatggc atcgctacag gccagcaatg
     3061 ccagcgccgc cgccagccag aaaccactgc cgaggctggt acgcgccagc gcactgccat
     3121 tttgcgccag ttgggtcgcc gcctttccag ctccccacac caacaatacg aagacgaatt
     3181 gcgccagaat gagtgcaaaa atgctgcctt ttttagcggg gacaaaacag gctgtcagcc
     3241 aggcgcaacc aacgcccacc agcatccaga tcgtttgcgg ccacagttgc cagagatgaa
     3301 gcccttcacc cgagactaaa cgattaggcg cgtaactgat aaacggcagc gccgctgcga
     3361 ttgccgtcag caacaacagc agcgccagaa caggattaat acgtagataa gtcacgggaa
     3421 attacttcgc ccaccctttt tgtttcaggt agtcggcagc cacttttttg gcatccagtc
     3481 cttccactgc aatgctggca ttcagttgct gcaatgtttt tgcatcgagg ctggcgaaga
     3541 ctggctgtag ccactgtgcc atttgcggat actccttcaa caccgattca cgcaccactg
     3601 gtgcaggcgc gtagataggt tgcacgcctt gcggatcgct taaggtttgc agccccagcg
     3661 ccgcgaccgg agcgtcagtg ccgtaagcca ttgcggcatt aacgccagag gtttgctggg
     3721 cagcggcttt aatcgtcacc gccgtgtcgc caccagccag tgacagcaac tgatcctgac
     3781 cgagcttaaa gccataggct ttttcaaacg cgggtaacgc atcggcgcgt tcgataaact
     3841 ctgccgaggc tgccagtttg aaggtgccgc cctcttgcag ataacgactc aggtcggcaa
     3901 gcgaagtgag tttgtttttc tctgccacat cctgacgcac ggcgatagtc caggtgttat
     3961 ttgcaggcgc gggcgtcagc cagattaact tgttgtgctc cgcatcgagc tttttgactt
     4021 tctcgtagcc ctgcccggca tttttccacg ctgcatcgtt ttcatcttta aagaagaaag
     4081 cgccattgcc ggtatattcc ggatagatat ccagttcacc ggaagtaatc gccccgcgca
     4141 ccacaggagt cgttccgagt tgcactttat tcaccgtcgg tacgccgtgg ctttcgagta
     4201 cctgcaagat gatgttgccg agcagcgcgc cttcggtatc gatttttgaa ccgactttaa
     4261 cgggggaagc cgcctgtagc ggcaggctca cggctgccaa cataaccagt gaacctgccc
     4321 agagctttaa gagtggcatg atgctttcct cattctttta ctgttgtttt cagcgaatta
     4381 agagaaaagc atagttgata atgacagagt tagcctgagc ggaaggattc agttgcagaa
     4441 tcagataaat acttaagagg catattcggt ccggcgtttc ctgccggatg cggcgcgagc
     4501 gccttatccg gcctacaaag ggcgaaaatt cagcaagtta agatatgaac atcaggcaaa
     4561 gggcgcaaac gtcgcgccct tactaaagca ttacagcaac tcaaactcgc ctttcttaac
     4621 gcgtgcggaa tcagtgccga taaatacatt gaacttgcca ggctcggcgt catatttcat
     4681 ctgttgattc cagaacttca gcgcctcaat atcgatcggg aagctgacag tctgagtttc
     4741 gcccggtttc agggtgattt tctcaaagcc tttcagctgt ttcacagggc gactcatgga
     4801 agccgtcaca tcctgcaagt acatctgcac taccgtggcc ccctcgcgct taccggtgtt
     4861 cgtcacctgc acgctggccg tcactttgcc gtcacgcttc atggtcggcg cagaaagttt
     4921 cacatcagag acggtgaaag tggtatagct cagaccatag ccgaacggat aaagcgcccc
     4981 gttagcttca tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat tatacggacg
     5041 accggtgttc agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggacat
     5101 tggcagcttg ccggacgggt tgtaatcgcc aaacaataca tcggcaattg cattaccgcc
     5161 ttcagtcccc gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag
     5221 cgccagcgga cgcccgttca tcagcaccag caccagcggt ttaccggtgg ctttcagcgc
     5281 cgcaatcaag tcacgttggc cttgcggaat agtgatatcg gtccggctgg aggcttcgtg
     5341 cgccatcccc tgtgcttcac cgactacagc caccaccaca tcagattgtt tcgccgtctg
     5401 caccgcttca tcaatcatct cttgcggcga acgcggatcg actttgaccg cttcttcata
     5461 ctgattcagg aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag
     5521 cactttaccg ttttcaccga cggaattttt aatcccggtc agtacggtca cggattgatc
     5581 ggcaacaccg gctgcggacc agctgcccat cacgtcacgt ttactgtccg ccagtggccc
     5641 aaccaccgca atggtggccg attttttcag cggtaacgtt tcgagacggt ttttcagcaa
     5701 cactaggctt tcgcgcgcca cttcacgcgc ttctttacgg tgcaggcggc tttcggcatt
     5761 ggtatccacc gggtcagact ctttcggccc caaatggctg tatgggtcgt taaacaaccc
     5821 catatcatat ttaacgttca gtacatggcg ggcagcatcg tccagctctg ccatggtcac
     5881 tttgccggat ttaatcaacc caggcagata cttcgagtag tactcgtcgc tcatactcat
     5941 gttgattccg gatttcagcg ccacgcgcac cgcatcttcc gggtctgccg ccgtgccatg
     6001 tttaatcagc tctttgattg caccgtgatc ggaaacggtg atgcctttaa agccccactg
     6061 gtcgcgcaga acatctttca gcagccagga atcggaggtg gctggcgtgc cgttcagcga
     6121 gttcagcgcc accatcaccg cgccgctgcc tgcgtccagc cccgctttgt acggcggcat
     6181 ataatcatta aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc
     6241 taccgcgccg tatgcggcaa agtgtttgac gctggtcatc accgagtaac gatctgccgg
     6301 gcttttaccc tgcatcgctt ccaccatggt tttacccatt attgaggtga gatacgtatc
     6361 ttcgccaaaa ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg
     6421 tgcccaggtc atattcaggc catcatctgc cgcttcataa gcagagacac gcccgaccgt
     6481 tttcaccgca tcgaggttaa aagacgaggc cagaccgagg ctaatcggga acaccgtgcg
     6541 ctgaccgtgc agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat
     6601 cacctgatcc tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa tcgccccaac
     6661 ctgaccgtct ttgatcatct cgcggatcgc ctctttcgga ttatccgggc ctacgctgat
     6721 taagcgcagc tgaccaattt tctcatcaac catcattttc ttgagcagat cggtgacgaa
     6781 cgcatcccgc gcttcaggcg ttaatggatg gttaccaaat aaatcatccg ccagtgctgg
     6841 ctgcaggacc agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct
     6901 tcctttgttg cccgacgtgg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat
     6961 gatggagaga gaaaaagaat gctcagttta ttgtctgaat tttcaaaata ttcactcgct
     7021 gaattgtcat acaaggcgct attctagttt gtgatatttt ttcgccacca caaggagtgg
     7081 aaaatgtctt ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg
     7141 gtcggtcatt cacacctgct caccgatccc gcaaaaacgg cccgctatcg caagggcttc
     7201 cgttctggtc agggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg
     7261 cgggtgctga aagcctgcgt caccgccgac aaaattattc tgatgcaggc tgccaataca
     7321 ggcctgaccg aaggatcgac gccaaacggt aacgactatg atcgcgatat cgtgatcatc
     7381 agcaccctgc gtctcgacaa gctgcacgtt ctcggcaagg gcgaacaggt gctggcctat
     7441 ccgggcacca cgctctattc gctggaaaaa gccctcaaac cgctgggacg cgaaccgcat
     7501 tcggtgattg gatcatcgtg tataggcgca tcggtcatcg gcggtatttg taacaactcc
     7561 ggcggctcgc tggtgcaacg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata
     7621 gatgaagatg gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg
     7681 gagcagatcc ttagcaagct ggatgacgat cgcatcaaag atgacgatgt gcgtcacgat
     7741 ggtcgtcacg cccacgatta tgactatgtc caccgcgttc gtgatattga agccgacacg
     7801 cccgcacgtt ataacgccga tccggatcgg ttatttgaat cttctggttg cgccgggaag
     7861 ctggccgtct ttgcggtacg tcttgatacc ttcgaagcgg aaaaaaatca gcaggtgttt
     7921 tatatcggca ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat tctggctaac
     7981 ttcgaaaatc tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa
     8041 aaatacggca aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc
     8101 ttctttaatc tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg
     8161 cattttactg accgtgcaat gcaaaaattc ggtcacctgt tccccagcca tttaccgccg
     8221 cgcatgaaaa actggcgcga taaatacgag catcatctgc tgttaaaaat ggcaggcgat
     8281 ggcgtcggtg aagccaaatc gtggctggtg gattatttca aacaggccga gggcgatttc
     8341 tttgtctgta cgccggagga aggtagcaaa gcgtttttac accgtttcgc cgctgcgggc
     8401 gcagcaattc gttatcaggc ggtgcattcc gatgaagtcg aagacattct ggcactggat
     8461 atcgctctgc ggcgtaacga caccgagtgg tatgagcatt taccgccgga gatcgacagc
     8521 cagctggtgc acaagctcta ttacggccat tttatgtgct atgtcttcca tcaggattac
     8581 atagtgaaaa aaggcgtgga tgtgcatgcg ttaaaagaac agatgctgga actgctacag
     8641 cagcgcggcg cgcagtaccc tgccgagcat aacgtcggtc atttgtataa agcaccggag
     8701 acgttgcaga agttctatcg cgagaacgat ccgaccaata gcatgaatcc ggggatcggt
     8761 aaaaccagta aacggaaaaa ctggcaggaa gtggagtaaa aattacggat ggcagagtat
     8821 cgccatccga attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc atttgggcgg
     8881 ctttttgttt tttatagctc aacgctgctg ccggcacagg catcacttta ccggtttcaa
     8941 tccaggtacg caggcggctg gcatcggcaa aatgggtata tttgccaaac gcgtccatca
     9001 ctaccagcgc caccggttta ttattgataa ccgtacgcat caccagacaa tggcccgccg
     9061 cattggtaaa gccggttttg gttaactgaa tattccagtt atcgcgatac accagatgat
     9121 tagtattgcg gaacggcagc gtatacgtcg ggttagagaa ggttgccata tcttcccggg
     9181 tagtacttaa ctgcccgatc aacggatatt gtttgctggc aatgagcagt ttggttaagt
     9241 cacgggcagt tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag cgcgtgttgt
     9301 tcattccgag cgatttcgct ttcgcattca ttgccttaat aaaggctttg taaccaccgg
     9361 gataatggtg cgcaaggctt gccgccgcgc ggttttctga agacatcagc gccagcaaca
     9421 gcatatcttt acggctgatt tcgctattca gtcgtacgcg cgaatagacc cctttcatct
     9481 ccggcgtctg gctgatatcc acttttagtt tttcatccag cggcagtcgt gcatccagca
     9541 caaccatcgc ggtcattaat ttgctgatag acgcaatcgg acgcaccaga tccgggtggt
     9601 tcgaatagat cactttgttg gtattcagat caacaatcat cgcgctaccg gaggcaattt
     9661 ccggttgtga agcggtggta gcggctgccg ttttcgcaac ggcctgcggt gcaaaaggca
     9721 cagccagcat cagggccagg ctaaataaag aaactcgaaa tttcggcatg atgagcattc
     9781 agatagtggt tcacgcgcac gggttgcgca ccgccggagt aaggatttac tgaggctagc
     9841 gacgccatca taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag taaatgctgc
     9901 attgctgaca tttacgccag caatgcaacg tcaaagaact ttctagaaca aacgataacc
     9961 gtagccccat agtataacgg ttagggtgag cagcacttcc agtaccagca cgccaatcgc
    10021 cagcgtcgaa ctggagaagc taaggccttc ctctttgttg atattcagga agctcggaat
    10081 accaaggtaa agcaggtagc cggtgtaaaa cagcgccacc gtgccgacca gcgcacacaa
    10141 ccagaccagt ggataaagcg ccaccagacc gcttaaaaac agcggagttg caacgtagcc
    10201 cgcgaagacc atgcagtgcg caagagacgg acgctgcggg taattgcgcg ccatccacca
    10261 gatgacccgc cccatcaccg caaccccagc cagcataacg ccgtaaaaca agacagccag
    10321 cgccagtccg gtaaaccagg ataacttcag gatagtgcca tcgccaaaat tccagccaat
    10381 ctgtgtagtg ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca aaacgtggtg
    10441 ggtgtaatgg tgagaaatcg tttcgttttc gcgattaatc acctgcattt cacgatcggg
    10501 atgggaaaac agtccccaga catggctcat accgccccct tgttgtgagt tcatgaacct
    10561 gacagttcaa gtataagcca gcttgtgatt attttttgtt catccatgta atttgcaccg
    10621 tctgatgtat gtcctttttc agcgtgaatg attcacaggg tgtatgctta caggcaacca
    10681 aagggagaca gactggccta tggatctcaa tacactcatc tcacaatatg gttatgccgc
    10741 gctggttatc ggtagcctag cggaaggtga aaccgtgact ttgctgggag gcgttgcggc
    10801 acatcagggg ctattaaagt tcccactggt ggtactttct gtggcgcttg gtggcatgat
    10861 tggcgaccag gtgctctatc tgtgcgggcg gcggtttggc ggcaagctgt tacgccgttt
    10921 ctcgaaacat caggataaaa ttgagcgggc gcagaaactt atccaacgcc atccgtatct
    10981 gtttgtcatt ggtacgcgct ttatgtatgg ctttcgggtg attggcccga cgctgattgg
    11041 tgccagccag ctgccgccga aaatctttct gccgctgaat attctcggcg catttgcctg
    11101 ggcgttgatt tttaccacta ttggttacgc tggtggtcag gtgattgcgc cgtggttgca
    11161 caatctcgac cagcatttga agcactgggt ctggttgatt ctggtcgtgg ttctggtggt
    11221 gggcgtgcgc tggtggctga aacggcgcgg gaagaaaaag ccggataatc aggcgtaaag
    11281 cagttgccac agataagcat tcactccaca tccggcaccg ggtgttccat gcctgatgcg
    11341 acgctggtgt cttatctggc ctaacccatt gaaatatcca cgcacctcac tgcgctattc
    11401 tgggttgaac tgcggattcg ccaacataaa gccgccatcc actatcaacg actgccctgt
    11461 cgtgtaattt gcgccctccg aacaaagcca caccaccagg ctggcaatct catgcgttgc
    11521 gccaaaacgc cgtagaggaa tcgaaggctc cgcgtcgggc ttcacgtcgc tatcatccat
    11581 gctgttcatt ggcgtggcta tcgccccagg cgcgactgcg ttcaccaaaa tcttatgcct
    11641 gaccaactcc agcgccatcg ttttggttaa cccgccgagc gcatgtttag cggctgtgta
    11701 ggcgctggca tccggcagcg gcgtatgttc atgtaccgac gtaatgttga tgatgcgacc
    11761 gccctgccct tatttcacca tctgacgagc cgcaatttgc gagcataaaa atgcaccatc
    11821 aacatcaacg gtaaaaatct tgcgccactc atcaaaagcc atatcaagaa acggcgcttt
    11881 ggtcattgca cccgcattat tcaccagcac atcaatacgc ccaaatcgct ggatgagtgt
    11941 ctccagcgcc tgcgcacctt ctggcagatt gccgagatcc agttgcacga tctccgcacg
    12001 tacgccgtgg gtaaccacct cacgcgcggt atcttttgcc ccttcttcat ctgagtgcca
    12061 ggtaatgcca atatcaaacc cctgctgcgc cagtaataac gcgcactctt tgccgatccc
    12121 cgaatcggaa gcggtaatta tcgcgaccta tgccatcgag ttctccactt aacgctgaat
    12181 aaacgttaag tatagaaggc gcatatcatc agcgtttgta acccccgccc agcgcaccgg
    12241 tgagtcgaat ggaggcatcc agccactgcc cttgcaataa caggccattg gcccgctcac
    12301 gcagcgccgg gattctggct tcgctgacgc gggaaccagc aatgatgccc gcgttaaagc
    12361 gggcctgcgc aagacctacc acacgcaagg catcgcgctc aatttgcgcc tgatgctggt
    12421 ttttctccgc cagtgtctga acctgactgg ctgcccgcgc cacgtcattc actgcttcaa
    12481 ccaccgcttt gttgtagctg gcgatagaca agttgctttc ggcttttgcg atatcgagat
    12541 tggcgttaag acgaccacta tcgaaaatag gtagcgtcag gcctgccgta acgcccattt
    12601 gctgcgcgga atgacggaac agatcgctta agtgcaacgc atcctgttgc aggaaggcca
    12661 tcaggttgat gtcaggataa aatgccgctt ttgccgcatc aatagtgctt aacgatgact
    12721 caacgtacca gtgcgccgcc tgcaaatctg cccgccgggc cagtaaggag taccccagtt
    12781 catcaggaag ctggcttgcc actttcggca acgcgaccgg tttaagcttc aatgacttag
    12841 tctggtgatt tgtgagtgca tttaaccgtg cctcaataat tttcattttc cccacgacat
    12901 cgttgagctg ctgccgggtt ttgctggcat taatatcggt ttccataccg tcaactgaag
    12961 aagtaatccc gttctgatat agctggcgat cggtcgcgat aatggtgttc tgctcttttt
    13021 ctatttgctg caagaccgtg tttaacgccg cctgggtttg ccactcccag tacaggcggg
    13081 ctacgctgcc agccagcaat tggcgggttt gctcgcgttc cgccgcccgt gctttaaccg
    13141 tacccaggcg ggcagtaacc tccgcccgat tctttcccca gatatcgaga tgccagcccg
    13201 ccgttaagcc aaaagttccg ttggtgtacc acggaccggt cgtacctgcg gccggatcgt
    13261 tcagagcaaa cggccccatt agcccttctg ccgacatttt ttgccgctcc atatccgccg
    13321 aaaagtcgat ctgcggacca tcctgagtgg caactgcctt cgcctgggct tcagctagct
    13381 gaatgcgctg ttcagccacc tgcatatccg gtgcgttctg tagtgcattg ttaattaatg
    13441 aagtgagctg attatcgtga tactccagcc accattggct gtctggccaa ccatttttca
    13501 gcgccgtggg taatgcggtg tcaacttgtg cagcgggcgt ttgctggctt aacgcctggc
    13561 gggtttcatg cataggcgca cacccggcca gcatcagtaa cagcggaaaa caggcgatgg
    13621 ctggataaaa ggaatcacga ttcatggggg aataatcagg taagaaacgg tgcgcggaga
    13681 ttaccgtgtg ttgcgatata ttttttagtt tcgcgtggca atacatcagt agcaataaaa
    13741 cgacataccc agaaaaatat agactaagta aatgatatct tccgatttat cttaatcgtt
    13801 tatggataac ggcaaagggc ttcgtttttt cctatactta ttcagcactc acaaataaag
    13861 gaacgccaat gaaaattata ctctgggctg tattgattat tttcctgatt gggctactgg
    13921 tggtgactgg cgtatttaag atgatatttt aaaattaatt aatgtcatca gatccgaaaa
    13981 taacgaga
//
LOCUS       DADMEQ010000099        13891 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.100, whole genome shotgun sequence.
ACCESSION   DADMEQ010000099 DADMEQ010000000
VERSION     DADMEQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.100"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..915)
                     /gene="wcaD"
                     /locus_tag="JNZ05_002993"
     CDS             complement(<1..915)
                     /gene="wcaD"
                     /locus_tag="JNZ05_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HAY8566150.1"
                     /translation="MSTSIRICSYLLLLLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSL"
     gene            complement(890..2107)
                     /gene="wcaC"
                     /locus_tag="JNZ05_002994"
     CDS             complement(890..2107)
                     /gene="wcaC"
                     /locus_tag="JNZ05_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HAY8566151.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKHQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2104..2592)
                     /gene="wcaB"
                     /locus_tag="JNZ05_002995"
     CDS             complement(2104..2592)
                     /gene="wcaB"
                     /locus_tag="JNZ05_002995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HAY8566152.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2600..3433)
                     /gene="wcaA"
                     /locus_tag="JNZ05_002996"
                     /pseudo
     CDS             complement(2600..3433)
                     /gene="wcaA"
                     /locus_tag="JNZ05_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3526..5688)
                     /gene="wzc"
                     /locus_tag="JNZ05_002997"
     CDS             complement(3526..5688)
                     /gene="wzc"
                     /locus_tag="JNZ05_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HAY8566153.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPSVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQVQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5691..6134)
                     /gene="wzb"
                     /locus_tag="JNZ05_002998"
     CDS             complement(5691..6134)
                     /gene="wzb"
                     /locus_tag="JNZ05_002998"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HAY8566154.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6140..7279)
                     /locus_tag="JNZ05_002999"
     CDS             complement(6140..7279)
                     /locus_tag="JNZ05_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAY8566155.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLIPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7938..9521
                     /locus_tag="JNZ05_003000"
     CDS             7938..9521
                     /locus_tag="JNZ05_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY8566156.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9973..11826)
                     /gene="asmA"
                     /locus_tag="JNZ05_003001"
     CDS             complement(9973..11826)
                     /gene="asmA"
                     /locus_tag="JNZ05_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HAY8566157.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11848..12429)
                     /gene="dcd"
                     /locus_tag="JNZ05_003002"
     CDS             complement(11848..12429)
                     /gene="dcd"
                     /locus_tag="JNZ05_003002"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HAY8566158.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12521..13162)
                     /gene="udk"
                     /locus_tag="JNZ05_003003"
     CDS             complement(12521..13162)
                     /gene="udk"
                     /locus_tag="JNZ05_003003"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAY8566159.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVKVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13480..>13891
                     /locus_tag="JNZ05_003004"
     CDS             13480..>13891
                     /locus_tag="JNZ05_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566160.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPL"
BASE COUNT         3226 a         3667 c         3508 g         3490 t
ORIGIN      
        1 aagtgagcca aatctgacta caccgtcaat atgggtcaaa gaataaccga ccatcaccag
       61 cggaccgaca atacgataat acgatgacga tccttccgta cctaaatcgc ccagacgggt
      121 ggaaatataa ggaaacgcga ttaccacacc aaccaggaat acagccagag aaatcaatgc
      181 taacggtaat tttttcttaa tcgcctcttt attcagatat tgaaatgccc actccagcaa
      241 ataaaacagg ataaaggtca taacccctga aaacgatccg gataatatta tccctgcgag
      301 aatcatagca tctgttttag gcgttttgat accaaactgt ttgatgctga gccaaattga
      361 gattaatgcc agagcgaaaa atgccggttc gaaataaagt gctgtcgtgc gcttgccgcc
      421 gaatttaatg aaattcagca catagctgtt gctgtaaatc agatatttcg aaatcgactc
      481 cataatactg ctgccaccgg tgaggataat ttgcgccatc tccaccgccg ccagcgccac
      541 caccagtccg accaccagat aaaagaaacg taatatctta cgatggttgt gtggcgaaat
      601 tgttttaaaa cgaatactcc acaccatgcc aataatgatc acaatataga caaacagcat
      661 agttgaagtg acgtatttac cggcatccaa cgactgacca aacaggtagt taaacgccgt
      721 gagtcccgcg ccaatcccta aggcaatcat caatttttta atgctgatgc gttctaaaaa
      781 cagcagtaac aagacaggta aaaaagtgac gatggtgatg gggaaacttt cgccaagctg
      841 ggcgattttg acgttaacca gcaggtagat cagtagcagc agcaggtagc tacagattct
      901 gatagaagtt gacatactcc tccagcatct gttgtccact gtaggcggcg cgactgcgtt
      961 ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc gatttccggt ttgcttaact
     1021 gtaccagttg cagcacctct tcttcactga cggttttacc accggatttt tgcagcactt
     1081 cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc aatcgatagc gcctcacaca
     1141 aaatcagcgg gtagttatcg acacgagaac tgaacaccag cgcatccatc tgattgagcg
     1201 cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt aaccacgttg ccagcggtga
     1261 acggcgagaa cttaccaaag gtatgcagtt cgattttgtc gcccagcgcc atcatctcac
     1321 gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc atgcgccacc accgcgattt
     1381 tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc cagaatcgct tcggttgcca
     1441 tatcaatgcc attattgata atccggcaac gccctggccc gtacagacta ttgaaagcgt
     1501 cagccacatg ctggctgggg gaaataaact gacagcccag cgccagcatc tcgcggaata
     1561 actggtgttt gcccgccacc agctggtgcg cgcgatcaat cttcaccggc ggatagttat
     1621 ttaaggtcgg gcatttctgg cagcccgttt tccagccttc gcaaccgtcg gtaaaggcgc
     1681 agcgtccggt tacactccag tggtcgtgca gcgtccagac cagggtgaca tccggtttat
     1741 ggtttttcac tttttcgcaa aagcgcacca cgctgttaag attcaaccag tagctgtgca
     1801 gcacatgaaa atgcaggacc accggacccg gtgtgcgagt aatggtgcga tataactcat
     1861 tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa cgcaatgttc gccatcgcgg
     1921 tcatccgcgg cgtatgtttg atgacctgcg gatagttctg atggctgaca ctctctttgc
     1981 cgcctttgcc gtagccgtag acaaaatgtg acgccagccc ctgttgcagc gcacgctggt
     2041 ggagatctaa cgccacacct gctgccccgc cttccgccag tcgcacatta aattgcagaa
     2101 tattcattta attaccttca ctcgcgcttt ttctcccacc accagcgcgt tgtccgggac
     2161 agagtcgagc accacgctgc ccgcacccac ggtgacgttg ttaccaagcg tgatatcacc
     2221 aagaataatg acgttggcac cgagttcgac gccgttgcca atgtgtggac atgccatgtt
     2281 atcggcaccc cgattgccga tagtgacgcc gtggcgaatg gtgaaatcat cccccgccac
     2341 cacgttttta ttgatcacta cggcgtaacc gtgatggata gtaaagcggc ggccaatggt
     2401 cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg ataatgcgat acagcaccag
     2461 cagcggggcc gcccacagat tgttgaggac gttctttttg cgccacaccg aacaaaaatg
     2521 agcgacgcga taggcaagta ccatgcagca cgggcgtaaa ctccagctgt tggcgcgcag
     2581 atcttccagc atctttagcg ccccgaatac catcagccag acgtttgccg ttacgcaccg
     2641 acagcagcgt caacaaggtg cgccaggtca tacgtttatt gcggatctgg tagggggtaa
     2701 acagctgata ttttttactg gcgcggtcga atttgtcttt gtgcttgcgg taaaagtgga
     2761 agtatccgga gaattttttc ggtgacgagg tgatctgcat ttcgccgtga ttgatatgca
     2821 ggatctgcgt cgcctcttcc actttccacg gttcgccgta ctccaccacc atccgcagga
     2881 atatgtcgta atcctgtgct gctttcagtt cggtatcgaa caaacactct ttgaaacgcc
     2941 atgcccaggt aaagacctga ttgccaatga tattacgttt atagaacagg cggcgtgagt
     3001 acggcgattt gggatacagt ggtaagctcg ccggttggga ataaacttct ccctggcaaa
     3061 cgtagtcgtt agcgtacaaa aaggcgtgtg tgaccagttg ctgtttatgg gcgaggaaga
     3121 cgctcaggcg gttgggcgtc cattcatcat catcatcgat cccggtgatg tattcccctt
     3181 gcgccagcat aatcgcctgg ttacgcaccg cgcacgcccc gctgttaatg tcgttgtgga
     3241 tgtaagtgat acgcggatcg ttgagggcag tgacgtactg ttgcagttgt tcccaggagg
     3301 tggagcaatc atccacgatg atcatctccc agttgctgta gtcctggcgc aggaccgatt
     3361 ttatcgcccg aatcgccagt tgttggcggt tccaggttgg catatagatt gagatcagcg
     3421 gattgtcttt cataggttgt tctcccggct tatgagctgg tttgcttgcc ggatgcggcg
     3481 tgagcgcctt atccggccta cggggcggtg cgaatgcagg cgtcgttatt tcgcatccga
     3541 cttatattcg tattcgtaat agccataatc ctgatacgcg ctggcgcggc ggaagatgga
     3601 gttcagaatc acccctttca ccggaatacc gttttgctca aagcggctca gactggtttc
     3661 cacttctttc aatgtgttga ccgcataacg cgccaccatt aacgtggttc cgacatgacg
     3721 accaacaatt gccgcatcgg tcactgccag aatcggcggc gtatcaatca acaccaggtc
     3781 atagttttta ctcgcccagt tcaccagttc ggcaaagcgt tcgctcatta acagttcaga
     3841 aggatttggc ggcacctgac cgcgcgggat caggtcaaat ttggcaatag aggtcggttt
     3901 agcagcggta gtaatatcgc cctgaccaat cagaatctcc gacagcccat tgacattatt
     3961 ggtgcccaac agctcgtggg tgtagccttt gcgcatatcg cagtcgatca acaacacgcg
     4021 tttattggtc tggctgatca ccgccgccag gttggcgcag acaaaggttt taccgattga
     4081 cgggctaacc ccggtcatca tcaacacatt gttctgcacc tgcatcatcg cgaagtgcag
     4141 actggtacgc agactacgga tggcttcaat cgccagatcg gttggattcc ccactgccag
     4201 tagctggctc tgtttatagc gtttaacccc tttgatggtt ttgacgctat cgcgcgcttt
     4261 ctgccactcc gacagcggga tgctggcata gacgctgatt ccgtgttctt ccagcacttg
     4321 cgggctttcg atgccgcgat taaacaacga gcgcagcagc acacccacga tagagagcat
     4381 caggccaagg ataatcgcgc cgaggataat cagccctttc ttcggtttta gcacgctagg
     4441 ctgggtgatt gccgggtcaa taatgcgcac atcgccgacg gtgctggctt cggtgatttt
     4501 cagctcctgc tctttattca gcagttgcat atagacctgc tgaccagact cgacatcgcg
     4561 ggtcagacgg acaatttcct gctgggtttt cggcatcgcc gttacgcgac cgttaagttt
     4621 ggctttctct tcttccagcg cctgacgttt ctccagcagc gtgcggtacg ccgggtgaac
     4681 tttggtgtac agcttggaga tttccgcctc tttaaaggtc agttcgttca actgcgcgtc
     4741 gatgttcacc atcgaatcga gcaccgcttt cgcttcaagc ggcagatcaa cagaatcttt
     4801 atcctgacgg aaggcattca gtttgttttc ggcaacatcg aggcggctac gtacttccgg
     4861 taactgttgc gcgaggaaag cgaggctttt cgacgcttcc gccgatttgc gctcaatatt
     4921 ttgttcctga tagttacgag cgatgctgtt aagaatgtcg cggatctgtt cgcgatcttc
     4981 accggtataa gtcaggctca gtacgcctgc gtctttgccg ttctccgtta ccgtcaggct
     5041 gttttgcagt tggttgatca tccccaacgt ggagtatttg gtgacggtaa actcactgcc
     5101 cgggcgggcg tgaatggctt caaccatcag cgtgacgcct tcttttttca gcatctggcc
     5161 cgcttgccca cgggcgctaa agccgccatc actgctcagg gtgtagtttt tgtcatccag
     5221 cacattaagc gtaaacacct gatccgccat ctctttcggg cggttaaagg tagtcacttt
     5281 caccgtctcg ttctgacgcc ccatcaggcg atcccagccc gccccgaaaa tcgggaacgt
     5341 gtttttgctc accgcaatat cgaggtcgag atcgtccacc gttttaccaa gcaccagacg
     5401 cgaacgaatc aactggatct cggcgtccga tgcaggcggt ttgttggcta atgccgagcc
     5461 gatatcctgc accagcgaat tgccgctgtt ttgctcgatt tgtaccagtg cgtcggcact
     5521 ataaatcggc gtggcgaaga aagtgtaaac cacggcacag agggcaaata cgacggtgat
     5581 gccaatcacc caccagcgcg cttcaatgac ggtgccgacc aggcgaccaa tatcgatttc
     5641 atcactgccc gttaccggag cggcatgttg ttttactttt tctgtcattc ttatacctgc
     5701 tctgcgttca atgcctgcgc ccactggcgg gcagaccgtt caagtaatgt gtacaccgct
     5761 gcaaacgttt cccggctttt gcgatacgga tcggggattt cacattcgtt atcccagtga
     5821 ccaaacagca tcactttgcc gcgcatctcc ggtgccatct cgcataagcg ttcgatatgg
     5881 cgcttatcca tggtcaaaat caggtcatag ttgcgacaca ggcggcggct gatttgtcgt
     5941 gcacagtggc cttccagaga cagttgatgt tctgcggcga cgctgatagc ggtaggatca
     6001 gcgcccttac cgaccagcgc gccgagtcca gcggactcca ctttcagttc cgggtgataa
     6061 cgttgcagta agcgttccgc catcggggaa cggcaaatat tgcctacaca gacaactaag
     6121 atgttgttaa acatgacgat taccagttat gaatgtcgct ggctgtatcc gtcatgtaac
     6181 ggacaccgct aatagttggc agcaactgat tgatcagacg gttccagcgg gaaaccgggg
     6241 cggtggtgac atacaccacg tcataaggtt gcaggcggaa ttctgtcgcc atcaccagcg
     6301 acgtcgcatc ggacatatcc agctggtaga tattggcaat cttgccgtta cgcccgccct
     6361 cgcctttcag cggacgaatg acaaagatgc cgctggcgtt ggaggtggtc atgtcgatac
     6421 cttcggcatt gcccagagct tcagtcaggg tcatgccgct aaagtccatt ttcagggtgc
     6481 tctgtttctt cacttcaccc atcacaaata ctttcagatc atcattacgc ggcacgtaga
     6541 gaatatcgcc gggataaagc agtcggttct gattgaggtc gccgttttgc atcagcgcct
     6601 gtaaagaaat gcgttcttca cgaccattgt gtgttagcac cacgttgcgc cagtcagcgg
     6661 tgtcggtcag gccacctgcg gcgttgatgg cgtcgagaat ggtcagtggt acgttggtga
     6721 tcgcctgttg accggattta ttcacctgac ctgagatata ggccttttgt gagcggaagg
     6781 cggcgatatt aacgtccacc tgcgggtcag cgatgtatgt cgctaagcgc ccggtaatat
     6841 cactgcggat ttcagcgagc gttttcccga ctacgtgaac cttgccgata tacgggtaaa
     6901 acatagtgcc gtcaggctgt acccagttgc cggtgtcgct ggagctgcgg tactgaccag
     6961 ctggcgtggt gagttccggg tgatcccaga cggtgacatt aagaacgtcc cccggcccga
     7021 cgcgatactg gtaattcgcg atctcgcttt ccagcgtcat attggggcgc gctacattcg
     7081 ggcgtgggcg taattggtca atcaggcgcg ggatcagcgg ataaacattc accattttgt
     7141 cgagatcgaa atcagcgtcc tgctgtttga tgacgtcttt gcccatcgtc gacatattgc
     7201 tgcccggaag tactgtgcaa ccgcttatca aggttactga caccaataat ggcatcaatt
     7261 tcattttgga tttcatcatt gtttatttat cactttggca gagtaattat cctgtgcact
     7321 attaatagca atgtcgccat gcacatttac cttgcagtta attgaataaa aatttaactg
     7381 gcatcagtcc taaaaaaatt gatttcatcc gcaggctatt gacagaataa ttcagactgg
     7441 tctttcaggc atccagacac gctaccgccc ctggcttttt agctaccaat acactgattg
     7501 agcttaattt ttcaaaccct ctctgcatac aatcgttgat gagaaagggt tattgcggaa
     7561 attaacttcc gaatataagg tgacattatg gtaattgaat attggctcgc caataatgca
     7621 ggaggaagtg ttacagctaa cggaatagca ggcaaggtaa caattcggca attggctatt
     7681 tttaagaatt atattaagtg tcattcaata tggtttttag gagtttcttt aggttgacaa
     7741 tatttaatat tgcgtctcca catgcgatat ttcttaaata atgttttatt attaccgctt
     7801 ttaattcagg gataatgtga ggttattatc ctcaaataat atgagataaa tagtgctgca
     7861 acattgcatt tttgccccga tttatccatg atcgaattgt gacatttgtc atacaacgaa
     7921 taggttttgt acttactatg gaatggattg ccgatccgtc tatctgggcc gggttaatca
     7981 cgctgattgt gatcgaactg gtcctcggca ttgataacct ggtctttatt gccatcctcg
     8041 ccgaaaaact accgccgaag cagcgcgacc gcgcacgggt taccgggctg ctgctggcga
     8101 tgttaatgcg cctgttactg ctagcgtcaa tctcctggct ggtcaccctg actcaaccgc
     8161 tgttcagctt ccgctcgttt acctttagcg cccgcgactt aatcatgctg tttggtggtt
     8221 tcttcctgtt gttcaaagcc acgatggagc tgaacgaacg gctggaaggg aaagacagca
     8281 ataatcccac acaacgcaaa ggtgcgaagt tctggggcgt ggtgacgcaa attgtggtac
     8341 tggacgccat tttctcactc gactcagtga ttaccgccgt cgggatggtc gaccatttac
     8401 tggtcatgat ggccgccgtg gttatcgcta tcagcctgat gttgatggcc agcaagccgt
     8461 taacgcaatt tgttaacagt cacccgacca tcgttattct ctgcttaagc ttcctgttga
     8521 tgattggctt tagcctggtg gcagaaggtt tcggcttcgt cattccgaaa ggctacctgt
     8581 acgctgccat tggtttctcg gtgatgatcg aggcactcaa tcagttggct atctttaacc
     8641 ggcgacgttt tctttccgct aatcagacgc tacgccagcg gactaccgaa gcggtaatgc
     8701 gcctgcttag cgggcaaaaa gaagatgcgg aactggacgc cgaaaccgcg tccatgctgg
     8761 tggatcatgg aaaccagcag atctttaatc cgcaggaacg gcggatgatt gagcgggtac
     8821 ttaatcttaa ccagcgtacc gtcagcagca ttatgacgtc gcgccacgat attgagcata
     8881 tcgatctcaa cgcgccggaa gaagaaatcc gccagttgct ggagcgaaat cagcatacgc
     8941 ggctggtggt taccgacggt gatgacgcag aagatttgct cggtgttgtt cacgttatcg
     9001 acctgctaca acagtcactg cgcggcgaac cgctcaacct gcgggtgttg attcgccagc
     9061 cgctggtgtt cccggaaacc ttgccgttgt taccagccct ggagcagttc cgtaatgccc
     9121 gcacgcactt tgcttttgtg gtggatgagt ttggctcggt ggaagggatt gtgacattaa
     9181 gtgacgtcac tgaaaccatt gccggtaact taccgaacga agtggaagag atcgacgccc
     9241 gccatgatat tcagaagaat gcggacggtt cctggacggc gaatggtcat atgccgctgg
     9301 aggatctggt gcaatatgtg ccgctgccgc tggatgaaaa acgtgaatat cacaccattg
     9361 ccgggctgtt gatggagtat ttgcagcgta ttccaaaacc tggcgaagaa gttcaggtgg
     9421 gggattattt gcttaaaacg ttgcaggtgg aaagccatcg cgtgcagaag gtgcagatta
     9481 taccgctgcg taaggatggc gagatggagt acgaggtgtg atggtatcgg ccattttacc
     9541 caatgctgcg taacgcctta taacttcagc aatgaagcgt tgtctgtggt aaacggacat
     9601 cacagcgggg gtgtgggtcc ggctgtaaat cgccgaatcg tttcctgtag gctggggcag
     9661 cccacatgga tgtgggctga gggcgcgttt tacagggatg ttacctcgcg cccgtacccg
     9721 atagccgaaa ggaataagat gagggaaccg cgccagcggc gatttaccgc cgggagcccg
     9781 ggttgccagg gtggtggcga ttgagccacc ctggcacgtt caccggcttg agcgttccat
     9841 agtagcgagg aacatcaagt gaacggaaca gccttcgatc ttacatattc agaatataat
     9901 agtgccgccg cgaacgcctg atgcgacgct ttacgcgtct tatcaggcct acaggacagg
     9961 tcacctggta acctacatct tctccagcaa cttcttcaca tctttgccat tgcgggaatc
    10021 tttattccgc tcggcccagt cattcaagcg acgtttcgct tcgtcctgta gatgtttgcg
    10081 cagtaactga tccacttgca ggctgtaatt gagttgctgc cagttgccat aaacccgcag
    10141 cggtactggc gtttctttga ggaaatcaat cagtttgctt tccccgttcc agccacccac
    10201 gacccgaata tcaaactggg tgtcgcaggt ttgatctgcc agattcaaca tgccttcccc
    10261 tgtcagcgcc agcacaggcg attgaccttg catgtcgttt aacgtcacga cgccatcctt
    10321 caacgtcaaa tcggtggtaa agcggtcaag ccgcgtcacg ttatcgaagt tttcagcggc
    10381 cttcacatca ccgccattac gctctaccgc ttgctgaatc atctgctgga agttcatccc
    10441 ttccatacgt gtgtcggtca tctcaacatg cgcctgccct tgccagttgt ggcggaatgc
    10501 gtcggcatct atgtcagcac cggagaaatc accagccagt gacatttttc cggtcaacga
    10561 aatcggatag ttaaacgcct tcagaatggt accaatctca acgttctcca gccgtggctg
    10621 gaagtttatc cgcggattta ttgatgtcgc gtccagcgtg cccggcagtg aaacctttcc
    10681 accgttaagc ttgccctgca gttgagtaat ttccagcaaa cccgacttgt tggtcatttg
    10741 cgtggcaaca tctgtaaaat tcattccgcg ccagcgcacg ttactggcct gcaacaaaat
    10801 atcagctgta aagccttgca gtccctgata ggccggttca tcaatacgcg aagaaatgac
    10861 cgggcgcggc aacgttgatt ggctctgccc ctgctgcgcg gcaccgtttt caccattcgc
    10921 ggtttcatta agcgggatga ggttgtcaag attcagttgc gggaattgca gtctcagctg
    10981 ccattccggt ttctctgtca gcgtgacctg tgcttgcccg ctcagcgtac tgtcattggc
    11041 ggtcaaacta atttggttaa atgaaaggcg tttatgtgac tcctgccact gggactggaa
    11101 actcccctgc ccctgaatac cttgttttgg taaatcggca ccctgcaact gccagttaat
    11161 ttgttcaata gccgctgtta aatcatgcgg ataatcagaa gcatctaccg taccgttaag
    11221 ggatatcgtg agatcgcgct gatcgcgatt aacccgcccg gagaactcaa atgagccacg
    11281 atgttgggga tcttgttcca tttgcaggcg gatattgcgg attgtcacct gctcgtcatc
    11341 ttcatgctgg aacaccagaa cgctgtccgc caccttaaga ctggatatat caaacgacca
    11401 tccgcgatcg tctgacagat ccggcaaggt attgtcgcgc ggtgcaaccg gagcgtcttc
    11461 actgcgcacc gcttccgtct gcggcgtcag ttggatcact gcccctttta gcatcacctg
    11521 cttaacgctc agttgatgac tcagtagtgg taaaagcgcc acgtccagac gcatgttgtc
    11581 ggcgcgaacc agtggctggc ttgcgccctg ggcggtgaga gacattcgcc cggagaggat
    11641 actaagctgc ggccagacgt gccaacgcag tggcccgtcg agctgcaatt gataaccgct
    11701 acgtgcagca acttgcttga ccatatagtc gcggaaatca ttcggattca ccagcaacac
    11761 taacgcagat aacccggcca ccagcacgac caggagtatc atcagcgtcg tcagaaatcg
    11821 tctcatggta tcctcaatgg gcctgaatta gtctttatcg attcggctgg ctaccgcgcc
    11881 ctgctggttg cgatatttcg catcttcacg gcggttgtaa ggtcgcgccg ccgggccgga
    11941 aagcggctca aagctcagcg caccaattaa catgcccgga cgcagcgcca gcggcagctt
    12001 accggagttg tagaactcca gcacaatgca accagaccag cccggatcga tacggtgcgc
    12061 ggtgacgtgc accatcagcc ccagacgcgc caaagaggaa cgcccgtcca gccagcccac
    12121 cagatcggct ggcagcgtca ccgactccag cgtcaccgcc agcgccagct ctcctgggtg
    12181 aagatagaac gcctcgccct cgtcgagaac gatctcatcg ctcatcacgc ggtcaagcgc
    12241 ggcgctcact tcatctttgg gaccgctcag atcgataaac gctgccgtgt gaccacggaa
    12301 ggtacgaaat ttattgccca ggcgtacatc caccgtcgcg ccgttaatac gctccactgg
    12361 cggacgtggg ttgatcgaca aacggccttc atcaagccag gcttcaatat ctcggtcaca
    12421 cagacgcatg gcattttctc ctttcgcgca tcactccctt aacgccaatt acgtcaaggg
    12481 cataccagct tatcactgaa cggtacacaa tttatcaagc ttattcaaag aactgactta
    12541 ttttcgcttt caatatatcg atcgcgatgc ggtttttccc gccgcgcggc acgataatgt
    12601 ccgcgtattg tttagaaggc tcaatgaatt gcaggaacat cgggcgcacg gttttttgat
    12661 attgcgccat cacggaatcc attgaacgcc cacgctcgtt aacgtcacgc ttgatgcggc
    12721 gcatcaggca gatatccagc ggggtatcaa cgaaaatgga gaagttaagt tcgtcacgca
    12781 aacgcgcatc cgtcagcaac aaaatgcctt cgagaatgat gaccttcttc ggctcaacct
    12841 tcaccgtttc tttcatacgc gtatgttcaa catagctgta aaccggcagg tcaattgccg
    12901 agccgcgttt caacgcttgt aaatgctcaa gcaacaggct gtgatccatc gcgctgggat
    12961 ggtcgtagtt ggtcttaacg cgttcttcca tcgacagatg gctttgatct ttgtaatagc
    13021 agtcttcggg aattacgccg atgtgttcat caccgacttg ctcacgtaat tcacgataaa
    13081 gggtactggc aataagactc ttgccggaag ccgatgcgcc agcgataccg ataatgacgc
    13141 actgatgaga ctgatcagtc atatatttag cgacctgatt aacctggatg ttaggaaggg
    13201 ggcgacgaag cgccaaacgc ggcaattata gggatttcat ccgtctgata ccagtcgaat
    13261 agcgttgccg cgcgctcaga gttaattgtt gacaaagaat tcccggtggc aaattacgtt
    13321 gatcagtttt atgtaaggta aaaaatgtta tacgcagttg cgcaaattat ccgcctttac
    13381 gtcactttat gagcaattcg catataaaat gtaaaacttt tgtactagca taaacacaga
    13441 aacgaatact ggcgacctgg ccttgcggat aaagcggtaa taagcaaaca atcacagcat
    13501 gtattaattg ccctgcccca cccgctgctt cacctggtca gtttaggttt agtctcgttt
    13561 atctttaccc ttgtctcgct tgagctttcg cagtttggca cccaactcgc cccactgtgg
    13621 ttcccgacgt ccatcatgat ggtggcgttt tatcgccatg ccgggcgcat gtggccggga
    13681 attgcgctga gctgttcgct gggaaatatc gccgcatcca tcctgctttt ttccaccagc
    13741 tcgctgaaca tgacctggac gaccatcaat attgttgaag ccgtggtcgg ggcagtgctg
    13801 ctacgtaaat tgctgccgtg gtataacccc ttgcaaaatc tggctgactg gctgcgtctg
    13861 gcattcggca gcgccattgt tccgcctctg t
//
LOCUS       DADMEQ010000100        13825 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.101, whole genome shotgun sequence.
ACCESSION   DADMEQ010000100 DADMEQ010000000
VERSION     DADMEQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13825
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.101"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            166..1419
                     /gene="kch"
                     /locus_tag="JNZ05_003005"
     CDS             166..1419
                     /gene="kch"
                     /locus_tag="JNZ05_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HAY8566161.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1474..1647)
                     /locus_tag="JNZ05_003006"
     CDS             complement(1474..1647)
                     /locus_tag="JNZ05_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HAY8566162.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1790..3250
                     /gene="cls"
                     /locus_tag="JNZ05_003007"
     CDS             1790..3250
                     /gene="cls"
                     /locus_tag="JNZ05_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAY8566163.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3285..3614
                     /locus_tag="JNZ05_003008"
     CDS             3285..3614
                     /locus_tag="JNZ05_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HAY8566164.1"
                     /translation="MDMDLNNRLTEDETLKQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3667..4671)
                     /gene="oppF"
                     /locus_tag="JNZ05_003009"
     CDS             complement(3667..4671)
                     /gene="oppF"
                     /locus_tag="JNZ05_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HAY8566165.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4668..5681)
                     /gene="oppD"
                     /locus_tag="JNZ05_003010"
     CDS             complement(4668..5681)
                     /gene="oppD"
                     /locus_tag="JNZ05_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HAY8566166.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTLGRLRACFKPVE
                     ELL"
     gene            complement(5693..6601)
                     /gene="oppC"
                     /locus_tag="JNZ05_003011"
     CDS             complement(5693..6601)
                     /gene="oppC"
                     /locus_tag="JNZ05_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HAY8566167.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6616..7536)
                     /gene="oppB"
                     /locus_tag="JNZ05_003012"
     CDS             complement(6616..7536)
                     /gene="oppB"
                     /locus_tag="JNZ05_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HAY8566168.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7622..9253)
                     /gene="oppA"
                     /locus_tag="JNZ05_003013"
     CDS             complement(7622..9253)
                     /gene="oppA"
                     /locus_tag="JNZ05_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HAY8566169.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(9990..10637)
                     /gene="ychE"
                     /locus_tag="JNZ05_003014"
     CDS             complement(9990..10637)
                     /gene="ychE"
                     /locus_tag="JNZ05_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HAY8566170.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11114..13789
                     /gene="adhE"
                     /locus_tag="JNZ05_003015"
                     /gene_synonym="adhC"
     CDS             11114..13789
                     /gene="adhE"
                     /locus_tag="JNZ05_003015"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAY8566171.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3628 a         3426 c         3221 g         3550 t
ORIGIN      
        1 gttcaggaaa aaagcccttt cctgacgcag agttgctcac tcaaaacctt tgattactgc
       61 ggataaagtc atctactatt gtttaacaca ttcacgcgct gcatgtatgt tcagaaaatg
      121 tgcacgctaa gataattaaa caaaattgtg gttacagagg tatcagtgag tcactgggct
      181 acattcaaac aaacagcaac aaatttatgg gtgaccctac ggcacgacat tctcgcgctg
      241 gccgtctttt taaatggatt gcttattttt aaaacaatct atggtatgtc ggtcaatttg
      301 cttgatattt tccatatcaa agcattttca gagttggatc tctccttgct ggcaaacgcc
      361 ccactattta tgctcggcgt ctttcttgtc ctgaactcca ttggcttact gttccgggca
      421 aagctcgcat gggcaatcag tatcattttg ttgttgatag cgctaattta caccctgcat
      481 ttttatccct ggctgaaatt tagtattgga ttttgcattt ttacgctggt gtttttgctg
      541 atactgcgca aagacttctc ccacagtagc gccgcagccg ggacaatttt tgcatttatt
      601 agtttcacga cattactgtt ttactcaacc tacggtgcac tttatttaag cgaaggtttt
      661 aatccgcgaa tagaaagttt gatgaccgcg ttctattttt cgatagaaac catgtcaacc
      721 gtcggctatg gagatattgt ccctgtttct gaatcagcac gattgttcac tatttcggtc
      781 attatttccg gcattaccgt ttttgccaca tctatgacct caatttttgg tccgcttatc
      841 cgcgggggat tcaacaaact tttaaaagga aacaatcata caatgcatcg taaagatcat
      901 tttattgttt gcggacattc gattctcgcc atcaatacga tcctgcaact gaatcaacgc
      961 ggacaaaacg taacggttat cagcaacttg cctgaagatg atatcaagca acttgagcaa
     1021 cgcttaggcg ataacgctga tgttatcccc ggtgacagta atgacagttc agtattaaag
     1081 aaagcgggaa tcgatcgttg ccgggccatt ctggcgctga gtgataacga tgcagataac
     1141 gcgtttgttg tactctcggc aaaagatatg agcagtgatg tcaaaacagt tctcgccgtc
     1201 agtgatagca aaaacctgaa taagattaag atggtacatc cggatatcat tctctcaccg
     1261 caactgtttg gcagcgaaat tctggcgcga gttttaaatg gtgaagagat taataatgat
     1321 atgctcgttt caatgttgtt gaactcaggt catggtattt tcagcgataa cgatgaacaa
     1381 gaaacgaaag ctgacagtaa agaatcggcg caaaaataga gtttaacgac ggaagagtaa
     1441 acttattggg caccgcaatg gtgcccagta catttagata ttgtagatcg caaacaataa
     1501 cgagttacgc tgtttgttta gaatgcactt cctgatgctg tggatacgca tatttcggcg
     1561 cgattgcccc tcaagccaac gcgatttacg tcggctagcc tgacgctgca tgcgccagcg
     1621 ccccacttcc gttctactcc gcttcatgtt aactactcta tgcaataaca gaatggtcat
     1681 tatacccccg cccccgacgc tgaccagtgc ttttcccgcg tttttatttg ccagatgaat
     1741 ccatatgcgt aaactcataa caatgcgctt tcaaaaggat ttctaatcta tgacaaccgt
     1801 ttatacgttg gtgagttggt tggccattct gggatactgg ttgctcattg caggcgtaac
     1861 tttacgcatt ctaatgaaac gacgcgcagt tccctccgcg atggcctggc tgttgattat
     1921 ttacattctg ccgttagtcg gaattattgc ctatcttgcc gttggcgagc tccatttagg
     1981 caaacgccgc gctgagcgcg ccagagcgat gtggccttcc accgcaaaat ggcttaacga
     2041 ccttaaagcc tgtaagcata tcttcgccga agaaaatagc agtgtcgctg cgccattatt
     2101 caagctttgc gagcgtcgtc aggggatcgc tggggtcaaa gggaatcagc tacaactgat
     2161 gaccgagtca gatgatgtga tgcaggcgtt aatccgcgac atccagctcg cgcgccataa
     2221 tattgagatg gtgttttata tctggcagcc cggcggcatg gcggaccagg tggctgaatc
     2281 attaatggcg gctgcacggc gtggtattca ttgccgattg atgctcgact ccgccgggag
     2341 tgtggctttt ttccgcagcc cgtggcccga gctaatgcgt aatgccggta ttgaagtggt
     2401 cgaagcctta aaggtcaatc tgatgcgtgt gtttttacgc cgtatggacc tgcgccaaca
     2461 tcgcaagatg atcatgatcg ataattacat cgcgtacacc ggcagcatga atatggtcga
     2521 tcctcgctac ttcaaacaag atgcgggcgt agggcaatgg attgatctga tggcgcgtat
     2581 ggaaggcccc atcgccaccg cgatggggat tatttattcc tgcgactggg agattgaaac
     2641 cggaaaacgt attctgccgc caccaccaga tgtcaatatt atgccgtttg aacaggccag
     2701 tggtcacacc attcacacaa ttgcttctgg ccccggcttt ccggaagatc tcattcacca
     2761 ggcattattg actgcggctt attcggcgca tgaatatttg atcatgacca cgccctactt
     2821 tgtgccaagc gatgatttac ttcatgcgat ttgcacggcg gcgcagcgcg gggtggatgt
     2881 cagtattatc cttccgcgaa aaaatgactc gatgctggtc ggctgggcca gtcgcgcatt
     2941 ctttacggaa ctgcttgctg ctggggttaa aatttatcag tttgaaggcg ggttactgca
     3001 taccaagagc gtgctggtcg atggcgaact aagtctggtt ggcaccgtta accttgatat
     3061 gcgtagtctg tggctgaatt tcgagattac cctggcaatc gacgataaag gttttggtgc
     3121 agaccttgcc gccgttcagg acgattatat ttcgcgttca cgtctgctcg atgcccgttt
     3181 atggctaaaa cgtccattat ggcaacgtgt cgccgagcga ctgttttact tcttcagccc
     3241 gttgctgtaa aacgtcgcca acagaggtta aacaggtagt gactatggat atggatctaa
     3301 acaatcgcct gactgaagat gaaacgctta agcaggccta cgatattttt ctcgaactgg
     3361 ctgccgacaa tctcgatcca gctgatgttc tgctgttcaa tcttcagttt gaagagcgcg
     3421 gcggcgcgga gttattcgat ccggcggaag actggcagga acatgttgat ttcgatttga
     3481 acccggactt ttttgccgaa gtggtgattg gtctggcgga cagtgaagat ggtgaaatca
     3541 acgatgtttt cgcccgcatt ctgttatgcc gcgaaaaaga tcacaaactt tgccatatca
     3601 tctggcggga ataaataatc gcttttagcg tgaaaaaggg ctgacaactg tcagccctta
     3661 ttgtttttaa agcggatcga ctttcaggca agaaacggcg tgtctgaaac tcccctccag
     3721 aacaggacgt gttttggcgc actccggacc ggcaatcggg caacgggtac ggaaaacaca
     3781 accggaaggc gggttgatcg gcgacggtaa ttccccttcc agtaactgga tggttttgtt
     3841 cttctccaga tccggatcag gtatggggac tgccgacatc aatgccctgg tgtaaggatg
     3901 tagtggattg tggtagacct catcataggt ccccagttct actgcatggc cgagatacat
     3961 caccaacaca cgatcggaaa tgtgttttac cacggctagg tcatgagcaa taaaaattaa
     4021 tgacaatccc atctcacgtt gcagctgctg gagcaggttg accacctgcg cctgaattga
     4081 cacgtccagc gccgaaaccg gctcatcgca gataatcagc ttcggttcaa gaataagagc
     4141 acgagcaatc ccgatacgct ggcactgccc accggagaac tcatgcggat agcggttaat
     4201 caggttaggc aataacccga ctttcagcat catcgccttc acgcgctcgc gaacttcctg
     4261 gcgtgacatt ttcggatgat aagtacgcag tggttcagcg atgatctcgc cgatggtcat
     4321 acgcgggttt agcgatgcca acggatcctg gaaaatcatc tgaatatcac tgcgaacggc
     4381 acgccattca tcgggcttca tgcccagcaa ctctttacct aaccaggcaa catgaccgtc
     4441 ggtcgccttg accaaaccga tgatggcgcg agcaaaggtg gacttaccgc atcccgattc
     4501 ccctaccaca cctaatgttt ccccttcata caggcgaaga gtgacaccat cgacggcttt
     4561 gagcgttttc ggcggttgcc agaaccactg tttgccatct ttgatttcaa agtgcacttt
     4621 aagatcggcg atttcaagga ggacttttct tccttcagtt acagcattca taacagttcc
     4681 tccaccggtt taaagcaagc acgcagacgg ccaagcgtaa actcttccag cggcggtgcg
     4741 ctactacaaa tttccatcgc atgtggacaa cgtggctgga acgggcaacc tttcggtaat
     4801 cgcagcaagt ttggcggatt acccgggatg gtcaacattg tttcaccttc cgcatcaaga
     4861 cgcggcaccg cgttgagcaa accgatagaa taaggatgaa cgggttgata aaagacatcg
     4921 cgcgcattgc catattccat cgtgcgcccg gcgtacatta ccagcacttt gtcacagatc
     4981 cccgccacca cgccaagatc gtgggtgatc ataatgatgg cagtattaaa ttcccgcttc
     5041 agttcattca atagcgtcat gatctgcgcc tgtacggtga cgtccagcgc tgtagtgggt
     5101 tcatccgcaa tcagcaactt aggtcgacat agcaatgcca tcgcaatcat gactcgctga
     5161 cgcatgccgc cagaaaattc gtgcgggtac attttcatgc gtttacgtgc ttccggcatt
     5221 tttaccgcat cgagcatccg caccgactct tcaaacgcct cagctttgct catgttctta
     5281 tgcagcatca gcacttccat caactgctca ccgacgcgca tatagggatt caacgaagtc
     5341 attgggtcct gaaaaatcat tgagatttgt tcagcgcgca gtttattgag ttcacgctct
     5401 ggcaaattga ggatttcacg cccattgaag gttgccgatc cgccaatgcg tccgttggca
     5461 gccaacaggc ccatcaacgc aaatgcagtt tgcgatttac ccgaaccaga ctcacctaca
     5521 atgcccagcg tttctccggc acgtagggaa aaatttaaat cattgaccgc cgtgacgtcg
     5581 ccgtccgggg tactaaaggt gacacgcaaa tctttcacgt tcagcagtgc gtcagcctgt
     5641 tgttgtgcga gcggcacagt tgcagtttca attacgctca tggctgcact ccttaacgat
     5701 ctttcgggtc gagggcatca cgcaagccat cgccgataaa gttgaaacaa aacagtgtca
     5761 ccacaaggaa tcccgctggg aacaacagta accatggaga gacttccatc gagttcgcgc
     5821 catcactcag caatgccccc cagctgctta acggctcttg cgtacccaac cccaggaagc
     5881 taaggaaaga ttcaaagagg atcatgctgg gcaccagtag cgatgcgtag accaccacca
     5941 caccgagtac gttcggcaca atgtggcgaa taacaatgcc cggcgtcgat acaccgccaa
     6001 cttgtgccgc ctcaataaac tctttgcgct tcagactcag ggtttgccca cgcacaatac
     6061 gagccatatc cagccaggaa accatgccaa tcgccacgaa aatcagcagg atgttttggc
     6121 cgaaaaaggt caccagcaaa atgacgaaga acatgaatgg gaaggagttg aggatttcca
     6181 gcagacgcat cattaccgaa tccactttac cgcccagata accggaaagc gaaccataaa
     6241 gtgtccccac gaccactgcc accagtgccg cagcaacacc gaccatgagt gagatacgcc
     6301 cgccaatcgc aacgcgcaca agcaggtcgc gaccggatga gtcagtacca aagtagtgac
     6361 cggactccat atccggggcg ctggacatca tcgcccagtc agtatcgtca taggcaaact
     6421 gggaaagcat cggtgccagg attacaaata acgcgatcag caccagcact atcagactgg
     6481 caaccgccgc acggttatgc ataaaacgtc gacgtgcgtc ctgccacaag ctgcgccctt
     6541 cgacctccag cttttcactg aaattttcca gcgtctcgct gtttttctta cttaacatca
     6601 tcgcgagctc cagtatcagt aacggatttt cgggtcgata accgcatata gcacatcgac
     6661 aatggcatta aacaaaatgg ttaacgcacc aaccaggatg gtcaggctta acactaagga
     6721 atagtcacgg ttcaatgcgc cattaacgaa cagttgccca atccccggca aaccataaat
     6781 ggtttcgatg accatagaac cggtaataat gccgacaaat gcggggccca tataggagag
     6841 cacgggtaac agagcaggtt ttaatgcgtg gcgtaaaatg atccgccgca taggtaaccc
     6901 tttcgcccgg gcagtacgaa taaagttgga gtgtaatact tcaatcatag agccacgggt
     6961 aatacgcgca atactggcga tataggcgag tgacaacgcc accatcggca atatcatgaa
     7021 tttaagcgcc ccaccattcc agccaccgcc cggcagccaa tgcaaaatga tcgcaaatat
     7081 catgactaat aatggcgcaa ccacaaaact ggggataaca accccggtca ttgccagccc
     7141 catcacggta tagtcccatt tggtgttttg ttttaatgcg gcaataacgc cagcactaac
     7201 acccagtatt accgcaagga aaaatgcggc ggctcccagt ttggcagaaa cgggaaaact
     7261 ggatgccacc aggtcattga ccgaataatc tttatattta aacgatggac cgaaatcgcc
     7321 atgcgccagt tgtttcaggt agctgaaata ctgtgtcatg atcggatcat taagatgata
     7381 tttcgcttcg atattggcca tcacttctgg cggtaaagta cgttcgccgg taaaagggct
     7441 tcccggcgcg aggcgcatca taaagaacga aatagtaata agaataaata gcgtcggaat
     7501 cgcttccaga cagcgacgta gaataaattt taacattgcc ctaccttctg gcctgtgcct
     7561 tctgtaatgc gataaaaatc agacaccgtg gagcaggaca ctcctgcccc acgtattgcc
     7621 attagtgctt cacaatgtac atattccggg tataggtatt atccagcgga tctttgccgg
     7681 tatagccacc aacccacggt ttcaccagac gcgcattcac gtagtaataa acaggaacaa
     7741 tggccgaatc cttatccagc tgttgttctg ctttagtgta cagagctgtg cgctgcgcct
     7801 cgtcagtcac tttcagcgtt tccgccataa tgctgtcaaa ggccgggctc ttataatgcg
     7861 cggtattcat cgagctgttc gacagcatgg tgttcaggaa ggaagttggt tcgttgtagt
     7921 ctgcacacca gcctgcacgg gccacatcaa aagtgccctg gtgacgggtg tcgaggaacg
     7981 ttttccactc ctggttaacc agtttgacgt ttacaccaat gtttttcttc cacaatgaag
     8041 aggcagcaat cgccagcttt ttatgcagat cggaggtgtt ataccgcagg ttgatggtca
     8101 acggtttgtc tgcggtataa cccgcttcag ccagcagttt tttcgcttct tcgttacgtt
     8161 tttcctggct ccagccaaac cattctggct gagtcaattt tgcgccatca gtatacggtg
     8221 gagtgtaacc ataggcgggc atgtcgccct gcgctttcac tttattaaca atgatatcgc
     8281 ggtccatacc tagtttcagt gcggtacgca cacgcacatc gttgaatggc ggtttctggt
     8341 tgttaatttc gtaatagtaa gtgcacaggt atggatcaac gtgaacttcg tccgggatct
     8401 cttttttcag cttctggaac aattcgatcg gcatgttgtt atacgtcatg tcgatttcac
     8461 cactacggta gcggttgaca tcggtaactt cagaagcaat aggcaaatag gttacctggt
     8521 taataacggt tttcgcgttg ttccagtagg tcgggctgcg ttcaagaacg attcgttcgt
     8581 ttacgaccca atcttttaag gtataggcac cgttggtgac gatattacca ggctgggtcc
     8641 atttttcgcc gaatttctcg atagcggctt ttggcaccgg tgaagttgat gggtgaacaa
     8701 gtaatttata gaagtacgga acgggttcac ttaaggtgac ttctaatgtg tgatcatcaa
     8761 tagctttcac gccgagatcg gtaatcggtt ttttcccttc aagaatttca tcaataccgg
     8821 cgatatgccc atattgcaga taactggcat acggagaagc agtgttcgga tcaacagaac
     8881 gttgccagct atacacaaag tcttgtgctg tgactggcgt gccatcagac catttcgcat
     8941 ctttacgcaa atggaaggtc cagactttcg cgtctttatt atcccaggat tcagcgacgc
     9001 caggtgctgg atgaccgtca agatcgctga ccagtaagcc ttcaaacagg tctcggctga
     9061 tattagactc cggaacacct tcaattttgt gcggatctaa tgactgaact tctgaaccat
     9121 tgttacgtac cagtgtttgt ttttccgcca gtgtgacgcc tgcgggtaca tcagctgcca
     9181 gcgcgacatt ccctgccatt agcgcagcca gaacgccagc tgctactaaa cttctcttgg
     9241 tgatgttggt cattgttttt tggactccct cattataatt actggtatta accagcatgt
     9301 gtaatcccgg attgggaccc tgcacaggct cggagacttt ctgctgtcag gttactttat
     9361 cgttacgtat tactatcacc gactttattt attctggtga ttcagacgat caccttatgt
     9421 cgattcgtac tgtgtctccc ctgtcgagac acattattct ttcgcgacgc tctttaattg
     9481 ttaattattc tcattaccaa taaagaatcg tctggcggtt gcccggaaag taccaaatgc
     9541 ggttcttact cgccaataca ttttgcaaaa ttgttaagcg attctctttt attgaccgta
     9601 tcagaatcta tagcttgcat ccatgcctta acaaacatct ttcaattcaa tcaaatagag
     9661 attggcaagc atgtctatct tttctgatta cagttccagt agtgaaatgc acaataattt
     9721 aacaattgat tattatttag cactttcctc taccaaaggt agtggaatta ctaatatcat
     9781 ttcaattatt cttcagcaag cccaggatta tgatgtggcg aaaataacat gatggtgagg
     9841 agaagcagtt aaggaaatcc ttataactaa ctgtaatttg agaagaatat ttttacctat
     9901 tccttttgct tttgataact gcgcgggaac aaagtaaaaa taatgacgaa gtaaacggag
     9961 catggcagct ccgtttcatt tgaaaggaat taattaagca ggccggggaa aatcccctta
    10021 ataccagtaa cgataaattc aatccccaat gccatcagca atagccccat aatacgcgta
    10081 atcacgttga tgccggtctg gcgtaaaacc cgtaccagcc acggtgccat gcggaacaat
    10141 ccccagcaac ataaagcgaa caatgcaata gccacaaaga aaccaaacag atagctaatg
    10201 ctgtgataac gcgtacccca gacgatggta gaactgatcg cccctggccc cgccatcaac
    10261 ggcaacgcca atggcaccac accaatactt tcacgtaccg cggtttctga tttttcttgc
    10321 ttgttctgtt tatcctcgcc aagcttgccg ctgatcatcg acatcgctat cgtcaccacc
    10381 aggatgcccc cggcgatacg gaacgaatca attgatatac caaaaagttg tagaatcgtg
    10441 tcgccgagaa aaagcgagat ccacaagata atggccacag acaggttggc tgtaaggtta
    10501 gttttgtttc gcgctgccgc tgtctgataa ctggtcatgc tgataaagac gggaataatc
    10561 cctaccgggt tgaccagcgc aaataacccg atgaaaaatt tgaagtaaac gggaaaatca
    10621 aaaagggtct gaatcacggt tagctccgaa gcaaaagccg gataatgtta gccataaata
    10681 aggttgaaaa gacgcgctga caatacgcct tttgacagca tttttcacct cctaactact
    10741 taaaattgct atcattcgtt attgttatct agttgtgcaa aacatgctaa tgtagccacc
    10801 aaatcatact acaatttatt aactgttagc tataatggcg aaaagcgatg ctgaaaggtg
    10861 tcagctttgc aaaaatttga tttggatcac gtaatcagta cccagaagtg agtaatcttg
    10921 cttacgccac ctggaagtga cgcattagag ataataactc taatgtttaa actcttttag
    10981 taaatcacag tgagtgtgag cgcgaataaa cttttgattt tcattggtta agcaaatcat
    11041 caccgcactg actatactct cgtattcgag cagatgattt actaaaaaag tttaacatta
    11101 tcaggagagc attatggctg ttactaatgt cgctgaactt aacgcactcg tagagcgtgt
    11161 aaaaaaagcc cagcgtgaat atgccagttt cactcaagag caagtagaca aaatcttccg
    11221 cgccgccgct ctggctgctg cagatgctcg aatcccactc gcgaaaatgg ccgttgccga
    11281 atccggcatg ggtatcgtcg aagataaagt gatcaaaaac cactttgctt ctgaatatat
    11341 ctacaacgcc tataaagatg aaaaaacctg tggtgttctg tctgaagacg acacttttgg
    11401 taccatcact atcgctgaac caatcggtat tatttgcggt atcgttccga ccactaaccc
    11461 gacttcaact gctatcttca aatcgctgat cagcctgaag acccgtaacg ccattatctt
    11521 ctccccgcac ccgcgtgcaa aagatgcaac caacaaagcg gctgatatcg ttctgcaggc
    11581 tgctatcgct gccggtgctc cgaaagatct gatcggctgg atcgatcaac cttctgttga
    11641 actgtctaac gcactgatgc accacccaga catcaacctg atcctcgcga ctggtggtcc
    11701 gggcatggtt aaagccgcat acagctccgg taaaccagct atcggtgtag gcgcgggtaa
    11761 cactccagtt gttattgatg aaactgctga tatcaaacgt gcagttgcat ctgtactgat
    11821 gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa cagtctgttg ttgttgttga
    11881 ctctgtttat gacgcagtac gtgaacgttt cgcaacccac ggcggctatc tgttgcaggg
    11941 taaagagctg aaagctgttc aggatgttat cctgaaaaat ggtgcgctga acgcggctat
    12001 cgttggtcag ccagcctata aaattgctga actggcaggc ttctctgtac cagaaaacac
    12061 caagattctg atcggtgaag tgaccgttgt tgatgaaagc gaaccgttcg cacatgaaaa
    12121 actgtccccg actctggcaa tgtaccgcgc taaagatttc gaagacgcgg tagaaaaagc
    12181 agagaaactg gttgctatgg gcggtatcgg tcatacctct tgcctgtaca ctgaccagga
    12241 taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg aaaacagctc gtatcctgat
    12301 taacaccccg gcttctcagg gtggtatcgg tgacctgtat aacttcaaac tcgcaccttc
    12361 cctgactctg ggttgtggtt cctggggtgg taactccatc tctgaaaacg ttggtccgaa
    12421 acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa aacatgttgt ggcacaaact
    12481 tccgaaatct atctacttcc gccgtggctc cctgccaatc gcgctggatg aagtgattac
    12541 tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc ctgttcaaca atggttatgc
    12601 tgatcagatc acttccgtac tgaaagcagc aggcgttgaa actgaagtct tcttcgaagt
    12661 agaagcggac ccgaccctga gcatcgttcg taaaggtgca gaactggcaa actccttcaa
    12721 accagacgtg attatcgcgc tgggtggtgg ttccccgatg gacgccgcga agatcatgtg
    12781 ggttatgtac gaacatccgg aaactcactt cgaagagctg gcgctgcgct ttatggatat
    12841 ccgtaaacgt atctacaagt tcccgaaaat gggcgtgaaa gcgaaaatga tcgctgtcac
    12901 caccacttct ggtacaggtt ctgaagtcac tccgtttgcg gttgtaactg acgacgctac
    12961 tggtcagaaa tacccgctgg cggactatgc gctgaccccg gatatggcga ttgtcgacgc
    13021 caacctggtt atggacatgc cgaagtccct gtgtgctttc ggtggtctgg acgcagtaac
    13081 tcacgccatg gaagcttatg tttctgtact ggcatctgag ttctctgatg gtcaggctct
    13141 gcaggcactg aaactgctga aagaatatct gccagcgtcc taccacgaag ggtctaaaaa
    13201 tccggtagcg cgtgaacgtg ttcacagtgc agcaactatc gcgggtatcg catttgcgaa
    13261 cgccttcctg ggtgtatgtc actcaatggc gcacaaactg ggttcccagt tccatattcc
    13321 gcacggtctg gcaaacgccc tgctgatttg taacgttatt cgctacaatg cgaacgacaa
    13381 cccgaccaag cagactgcat tcagccagta tgaccgtccg caggctcgcc gtcgttatgc
    13441 tgaaattgcc gaccacctgg gtctgagcgc accgggcgac cgtactgctg ctaagatcga
    13501 gaaactgctg gcatggctgg aaacgctgaa agctgaactg ggtattccga aatctatccg
    13561 tgaagctggc gttcaggaag cagacttcct ggcgaacgtg gataaactgt ctgaagatgc
    13621 attcgatgac cagtgcaccg gcgctaaccc gcgttacccg ctgatctccg agctgaaaca
    13681 gattctgctg gatacctact acggtcgtga ttatgtagaa ggtgaaactg cagcgaaaaa
    13741 agaagccgct ccggctaaag ctgagaaaaa agcgaaaaaa tccgcttaat cagtagcgct
    13801 gtctggcaat ataaacggcc ccttc
//
LOCUS       DADMEQ010000101        13602 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.102, whole genome shotgun sequence.
ACCESSION   DADMEQ010000101 DADMEQ010000000
VERSION     DADMEQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.102"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(1..1178)
                     /locus_tag="JNZ05_003016"
                     /pseudo
     CDS             complement(1..1178)
                     /locus_tag="JNZ05_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544745.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1388..1831)
                     /locus_tag="JNZ05_003017"
     CDS             complement(1388..1831)
                     /locus_tag="JNZ05_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566172.1"
                     /translation="MNPSDAIEAIEKPLSSLPYPISRHILEHLRKLPRHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTVLPGHAAIPLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFFLTR"
     gene            2346..3188
                     /locus_tag="JNZ05_003018"
     CDS             2346..3188
                     /locus_tag="JNZ05_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566173.1"
                     /translation="MKKELFGRCPFDLDEDVKKIAAINKYIHAKFEEHKNKIDNLKGV
                     EQKLMVLQYFNVLNELSNQAISSLNIGAFSASEVLARVIIEQAANQFYIAIDDGENAQ
                     ALLKASKKLVHSNGKRWLECLKSKGMTNPAANERIRMGKELTDLFNRLWPNTPEYPGT
                     RKLFELIGWETHYHAHYVPLCDSIHTFSDDMVNIVSLYNAIQSDETTAIEFILAVKQE
                     NKRLAIYNFVIATALRCEALVNVFNSLGYQDIITEMTPTIDTVNQTIIRYDDFDHSRI
                     DDCQ"
     gene            3295..3558
                     /locus_tag="JNZ05_003019"
                     /pseudo
     CDS             3295..3558
                     /locus_tag="JNZ05_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            4185..4730
                     /gene="cobU"
                     /locus_tag="JNZ05_003020"
     CDS             4185..4730
                     /gene="cobU"
                     /locus_tag="JNZ05_003020"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HAY8566174.1"
                     /translation="MMILVTGGAWSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            4727..5470
                     /gene="cobS"
                     /locus_tag="JNZ05_003021"
     CDS             4727..5470
                     /gene="cobS"
                     /locus_tag="JNZ05_003021"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HAY8566175.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSCDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            5482..6561
                     /gene="cobT"
                     /locus_tag="JNZ05_003022"
     CDS             5482..6561
                     /gene="cobT"
                     /locus_tag="JNZ05_003022"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAY8566176.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            6623..7558
                     /gene="ldtA"
                     /locus_tag="JNZ05_003023"
     CDS             6623..7558
                     /gene="ldtA"
                     /locus_tag="JNZ05_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY8566177.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFASQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(7614..7689)
                     /locus_tag="JNZ05_003024"
     tRNA            complement(7614..7689)
                     /locus_tag="JNZ05_003024"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7654..7656),aa:Asn,seq:gtt)
     gene            8016..8933
                     /gene="nac"
                     /locus_tag="JNZ05_003025"
     CDS             8016..8933
                     /gene="nac"
                     /locus_tag="JNZ05_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator"
                     /protein_id="HAY8566178.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            9035..9985
                     /gene="cbl"
                     /locus_tag="JNZ05_003026"
     CDS             9035..9985
                     /gene="cbl"
                     /locus_tag="JNZ05_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HAY8566179.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(10023..10098)
                     /locus_tag="JNZ05_003027"
     tRNA            complement(10023..10098)
                     /locus_tag="JNZ05_003027"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10063..10065),aa:Asn,seq:gtt)
     gene            10103..11730
                     /gene="yeeO"
                     /locus_tag="JNZ05_003028"
                     /pseudo
     CDS             10103..11730
                     /gene="yeeO"
                     /locus_tag="JNZ05_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            11831..11906
                     /locus_tag="JNZ05_003029"
     tRNA            11831..11906
                     /locus_tag="JNZ05_003029"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11864..11866,aa:Asn,seq:gtt)
     gene            complement(12362..13078)
                     /locus_tag="JNZ05_003030"
     CDS             complement(12362..13078)
                     /locus_tag="JNZ05_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY8566180.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(13421..>13602)
                     /locus_tag="JNZ05_003031"
     CDS             complement(13421..>13602)
                     /locus_tag="JNZ05_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY8566181.1"
                     /translation="VSDKPLHGEIKLPGQANRFYEGAISEHLQIGIRAIDLLRAEGDR
                     LHSRKLRTFNEPPFR"
BASE COUNT         3478 a         2942 c         3369 g         3813 t
ORIGIN      
        1 cacaggtctg tcgccccttc agttgccagt tcgctgtctt tacagacgta atcttttttc
       61 atgcgaccga aggcttccct gacaaaatca tcgacctagg cttcatcaac aggaagggtt
      121 ccgtttttcc gtaactcatg aagcgcacgg ttagccaggt atgcctggcg tgaggatccg
      181 ggataaacgc gggaggcggg cagcacatcg ctgttcagca ttgccggtac cttctgacag
      241 actgcatcag ccccatcgcc attacggtct ttttcatcaa atttattgac cagcacaaac
      301 aggcggccga ctgacacatc agcaatggca ttaagctctt tacgaacctc ctcatcggct
      361 ttcgagttca tctgggtgta gtccatcacc gccagcaccg cagaggcttt ctgcagttga
      421 tcccgcatca tcacttccat ctgcggttgt ccggcttcat tagggccggg ggtatccacc
      481 agtgtgagtg ttccctggca ttcatccatc cccaccagat ggctgaattc cacgtcgatc
      541 accggcagtt tctgcacttc tgcgtattca tcaaaaggaa attcactccc cattgccgca
      601 gcaagacgaa ccagatcgtt taacgatgcc agtccggtaa agattccctc ctccccgtgg
      661 tattcatttt tgagccagcc atcatccgtc agaatatcca gcagttcgcg ggtatccccg
      721 gtttgctgca gggtctgtgc gacctgctgt cctgccgggg tggcgacttt ttcctgcagt
      781 gtgattaata aattgcggac aggctgaata tgttccagat ggagaaccgg ctcagttttt
      841 ccgggaacgt ggcggatgag cgtcggtaca gaggtcatgg ggcggttacg gttaggcaga
      901 atttcctgcc ccacaatggc gttgatggtg gttgatttgc ctgctttcat cgtgccgaca
      961 acagccagca ccatctccct gcgggcggtt ttgatttgtt cgccctccag ctccttaatt
     1021 cttttcagtg ctttctgttt atcaaaaagc tgcccgtttt cgttcttacc gtcagataac
     1081 acatccggct catcaaccat ttgccggagc agattgatgc tcagttgcaa aagccggtcg
     1141 gcttcatcac aaagcagggt gatgttcttt tcgtgcatta tttaaccttc ctgagacgca
     1201 ttttcatgac aaccacggag gaggccattt actacaagcg atcgtttata acgatcaata
     1261 taatagaaac gatagtcata ttctattaat atgaaaaaca caacgcaagg gggacaaccc
     1321 gggtatggct gcgtttgcat tgtgttttca tagagttaaa aatctgtaac gacaacccgg
     1381 cacatcacta ccgggttaag aagaaaatcc agttccatac cgcgcgggcc acagagacca
     1441 cggtgtcacg aacggcgcgc agggccgtac ggacaacaga ggcaacacag acactctccg
     1501 ccgtgtcaaa tatccggtcc accgcaccgg taaactgttc acgggtctga gtgcggacag
     1561 actccgtgcg cagttcgtcc tgcaggcgtg tcatcagagg gatggcggca tgtccgggaa
     1621 gcactgtcat gagtgcactg accggggtga cctccccctg aaacagtgca ttacagagtg
     1681 aggatttacc ggccccgctt ttacccataa tgccaggcac gggttcgtga cgggggagtt
     1741 tgcgcagatg ttccaggatg tgacgggaaa tcgggtaagg cagggaggag agcggttttt
     1801 caattgcctc aatggcatca gacggattca tacatgtatt cccagtgaaa aaagacaaaa
     1861 ccccgtgcac cggaaaggtg gcgggggatt cacggagata atatctttct gaaattattt
     1921 tgaatgcagg tatatgtggc tgatttgtat cagctaatga gtacaggtct gaatctaatc
     1981 tgacagtccg ctttgtgcca ggagcggact gacctgctcc ccgatgatta atacacagct
     2041 atgctagtaa tgtctgtaga tcgctgtgtg ttgcatctat cggttgaact cacagtccaa
     2101 accagactgt cagctttgat tagattctac ctactcaacc tgtcagataa ggtctgagct
     2161 aatacatcta atatatggct ttgtgcaggg gacggctatc gcgaacgtta ggctaggtat
     2221 ctgaatacgc acagttcact tggttgtact tcttgcactg atctgctatc ccgcacagct
     2281 atttataggt aatcttcagt gtataatctg ccgacttatg gaagcttgta caacaggaga
     2341 aatatatgaa aaaagaattg tttggacgtt gcccgtttga tctcgacgaa gatgtaaaaa
     2401 aaatagccgc gataaataaa tatatccatg caaaatttga ggagcataaa aataaaatag
     2461 acaacttaaa gggtgtggaa caaaaattga tggtcttaca gtactttaat gtactgaatg
     2521 aacttagtaa tcaggctatc tcttctttga atataggagc cttttcagca tcggaagtat
     2581 tagctagggt tataatagaa caggctgcca atcaatttta tatagcaata gatgatggcg
     2641 aaaatgcaca agcattactc aaggctagta agaaattagt tcatagtaat ggaaaacggt
     2701 ggctggagtg tttaaaatcc aaaggaatga ctaatccagc agcaaatgaa aggattcgca
     2761 tgggaaaaga actaactgat ctatttaata gattgtggcc aaatacacct gaatatccag
     2821 gaaccagaaa actttttgaa ttaattggct gggaaacaca ctatcatgcc cattacgttc
     2881 ccctttgtga ttcaatacat acattctctg atgatatggt aaatatagta tctctatata
     2941 atgcaatcca aagtgatgaa actactgcta tcgaattcat tcttgcagtt aaacaagaaa
     3001 ataaacggct ggcaatttat aattttgtaa ttgctacggc ccttcggtgt gaggccttag
     3061 taaatgtatt taactcttta ggttatcagg atattataac agaaatgaca ccaacaatag
     3121 atacagtcaa ccagactata attcgatatg atgattttga tcattccaga attgatgatt
     3181 gtcaataatt aattgacaga atacgtacat gcgcaacaac tagtgatagt tgacttattt
     3241 tcgctcttga gagacaacca tactcaaatc tcccacattg caggagattt gaatatgaaa
     3301 acgtcaccgt ggaataaaga ccgtaccatc ggtcaaaaaa ggccacttca gatatctcat
     3361 atctggggga tccggattag acttgagttg gaaggtaaaa cacgcgatct agctctgttc
     3421 aacatggctt tggacagtaa gctacggggc tgtgatttgg tcaaactcaa agtatcagat
     3481 gctgcatatg gcagttctgt ctcaagcaga gcaacggtgt tgcaacagaa aaccggcagc
     3541 cctgtccaat ttgagatgcc tttaataaca catcttttaa atgttgaaga attatcccgc
     3601 ctgctgaaga acgttgctct gagtgtgata aaccttttta ttcttgatga tgtcagcgag
     3661 aagatggaat atatccccga ctgggtcgtg gttacccggt taaacatctg gtggcgctaa
     3721 taaatctggc aagtcacatt ctgttgaatg ctgtgacagt gccttaccga gagagcgaaa
     3781 ttgtggtgtt ctagtgcata gtcgccacca ttccggataa ttctattgcg ttgttttaca
     3841 agttctttta cagcgcagat ctgtcgctga cgttgaatca atagcctgga tttcagaaca
     3901 caatgcccgt ttgctggttg gtccggcagt agcattaatt cattatctga atgaaatgtg
     3961 tcaatccaca gaaaccgctg tcaatgacgc attttgtcgg acgttaacac gagaaagtct
     4021 ggctattccg taatgccatc ttatttcgta atggattgag aatgctggat ttttaattat
     4081 attacgcgca gtattaatcg cgtaaatgtt ttgcggtcag caacggtggg ggatccattg
     4141 ctctgttcaa tgaataccat catacatttg aatcaggagt cggtatgatg attttggtga
     4201 cgggaggcgc atggagcggg aagagtcgcc atgcagaggt gcttattggg gactcttcac
     4261 aggttctgta tatcgctacc tcgcaaatcc ttgatgatga gatggctgca cggatagaac
     4321 atcatcggca aagccgcccg gagcactggc gcacagtgga gcgctggcaa catcttgatg
     4381 aattaattca tgcagacatt aacccgaatg aggctgtgtt gctggaatgc gttaccacaa
     4441 tggtgactaa tctgttgttt gattatggcg gcgataaaga ccctgatgaa tgggattatc
     4501 aggcgatgga acaggcgatt aatgctgaga ttcagtcgtt gattgctgcc tgccaacgtt
     4561 gccccgcaaa ggttgtatta gtgactaacg aagtgggaat ggggattgtg ccggagagtc
     4621 gtctggcacg acattttcgt gatattgccg ggcgggtaaa tcagcagttg gccgctgcgg
     4681 caaatgaagt atggctggtg gtttcgggta ttggagtaaa aatcaaatga gcaaattatt
     4741 ttgggcgatg ctctcattta ttacgcgtct gcctgtaccg cgtcgctggt cccagggact
     4801 ggatttcgag cattattctc gcggtattat tacttttcct ttgattggat tattgcttgg
     4861 cgcgattagc gggctggtct tcatggtgct gcaggcatgg tgtggcgcac cactggcggc
     4921 actgtttagc gtacttgtgc tggcgctgat gaccggggaa ttccatctgg acggcctcgc
     4981 tgatacctgt gatggcgtat tttctgcacg tagctgcgat cgcatgttgg aaatcatgcg
     5041 tgatagccgt ttaggcaccc acggcggtct ggcattgatt tttgtggtac tggcaaagat
     5101 tctggtgtta agcgagttgg ctctgcgtgg cgaaccgatc cttgcatcgc tggcggcagc
     5161 atgtgcggtc agccgcggga ctgccgcatt attgatgtat cgtcatcgtt atgcgcggga
     5221 agaagggctt ggcaatgtgt tcattggcaa aattgatggg cgacaaacct gcgtcacact
     5281 cggcttagcc gctatttttg ctgccgtatt attgctgggt atgcatggtg tggctgctat
     5341 ggtggtgacg atggtggcga ttttcatcct cggtcagctt ctcaaacgta cgctgggcgg
     5401 gcaaacgggc gatacgctgg gagcagccat agagcttggt gaactggtct tcctgctggc
     5461 actgctataa agagacatga catgcaaata attgccgatt tactgaatac gatcccagcc
     5521 atcaattctg ccgctatgtc gcgtgcacaa cggcatgttg acgggttact caaacctgtt
     5581 ggtagcctag gaaagctgga ggcgcttgcc atacaactgg caggaatgcc gggggtgaat
     5641 ggcataccgc atgtgggcaa aaaagcggta ctggttatgt gtgccgatca cggcgtctgg
     5701 gaggaagggg tcgctatttc tccaaaagaa gtgacagcca tacaggctga aaatatgacc
     5761 cgtggaacaa ccggcgtgtg tgtgctggca gcacaagcgg gcgctaacgt ccacgtaatt
     5821 gatgttggta ttgatactgc tgagcctatc cccgggctta tcaacatgcg tgtcgcacga
     5881 ggtagcggca atattgcttc agctccggca atgagtcgcc gtcaggctga aaagttgctt
     5941 ttggacgtca tatgttatac gcgggagctg gcgaaaaacg gtgtcacgct gtttggtgta
     6001 ggtgaactgg ggatggcaaa cacgacaccg gcagcggcaa tagtcagcac aatcactggc
     6061 cgggatcctg aagaagtggt tgggattggc gcaaacctgc cgacagataa actggctaat
     6121 aaaattgatg ttgtgcgtcg ggcgattacg ttgaatcaac caaatcctca ggatggtgtt
     6181 gatgtcctgg caaaagtggg tggatttgat ttggtcggaa tagctggagt gatgttaggt
     6241 gctgcttcct gcggtttacc cgtgttgctg gatggatttc tttcttatgc tgctgcgctc
     6301 gcagcctgcc agatgtctcc tgcaatcaaa ccgtatctca ttccttctca cttgtcggca
     6361 gaaaaaggcg cgcgtatagc gctctcgcat ttggggctgg agccttatct caatatggat
     6421 atgcgtttag gtgaggggag tggtgcagct ctggcgatgt ccatcatcga agctgcttgt
     6481 gcgatataca acaacatggg cgaacttgct gccagtaata ttgttctacc ggggaatacg
     6541 acttctgatt tgaacagcta aaaacgaaaa gtctgataaa agctatactt atcggatagt
     6601 attcgcgact taggaaaggg acatgatgcg tcgtgtaaat attctttgct catttgctct
     6661 gctttttgcc agccaaaata gcctggcggt aacttatcca ttacctccag agggtagccg
     6721 tttagtgggg cagtcgctta ctgtaactgt tcctgatcac aatactcagc cgctggagac
     6781 ttttgcctca caatacgggc aagggttaag taatatgctg gaagcgaatc cgggcgctga
     6841 tgttttttta ccgaagcctg gctcacaact caccattccg cagcaactga ttttgcccgc
     6901 cactgttcgt aaagggattg tggttaacgt cgctgaaatg cgtctttatt actatccgcc
     6961 agacagtaat acagtggaag tctttcctat tggtatcggc caggctgggc gagaaacccc
     7021 gcgtaactgg gtgactaccg ttgaacgtaa acaagaagca ccaacctgga cgccaacgcc
     7081 gaacacccgg cgcgaatatg cgaaacgagg ggagagtttg cccgcatttg ttcctgcggg
     7141 gcccgataac cccatggggc tgtacgcgat ttatattggc aggttgtatg ccatccacgg
     7201 taccaatgcc aattttggta ttgggctccg tgtaagtcag ggctgtattc gtctgcgtaa
     7261 tgacgatatc aaatatctgt ttgataatgt tcctgtaggc actcgtgtac aaattattga
     7321 tcagccagtg aaatacacaa cggaaccgga tggttcaaag tggctggaag ttcatgagcc
     7381 gctgtcgcgc aatcgtgcag aatatgagtc tgaccgaaaa gtgccattgc cggtaacccc
     7441 atctttgcgg gcgtttatca acgggcaaga agttgatgta aatcgcgcaa atgctgcgtt
     7501 gcaacatcga tcgggaatgc ctgtgcaaat tagttctggt tcaagacaga tgttttaaga
     7561 gcgttggtaa ttagaaagta aaaagcctgc tcgaaagcag gcttttttga atttggctcc
     7621 tctgactgga ctcgaaccag tgacatacgg attaacagtc cgccgttcta ccgactgaac
     7681 tacagaggaa tcgtgtgaac ggggcgcata ttaatggcag tgcatggtga tgtcaaaggg
     7741 tgaaatgcat ttttttgttt gtttgctgcc aatttcaaca aagtggtgag ctttcaatct
     7801 tattgggtaa tgaaccatcg tggtgcatac cctcctttta tagggcaggt gaatgcgact
     7861 gctgataaaa agggccaatg acgaatctat cataatctct tatttgacag ttggttagct
     7921 tgcgcatcaa cgccaaaata gaactggcag gcatcttgca tactggttgt aagtaatggc
     7981 ggcacttggg ccgattctta aaaaccggag gcaacatgaa cttcagacgc ctgaaatact
     8041 tcgtaaaaat tgtagatatt ggtagcctga cccaggctgc tgaagtattg catatcgcac
     8101 aaccagcgct cagccagcag gttgccatac tggaaggtga gttaaatcaa cagcttttga
     8161 ttcgcacaaa acggggcgtt acgccaacag acgccggaaa aattctctat acccatgcac
     8221 gggccatttt acgtcagtgt gagcaggccc aactggcggt gcataacgtt ggtcagtcat
     8281 tatcggggca agtctcgatt ggctttgcgc caggaaccgc tgcgtcatcc atcaccatgc
     8341 ccttattaca ggcggttcgc gctgaattcc cggaggtcgt tatctatctt catgaaaata
     8401 gtggcgcagt gcttaacgaa aaactgataa atcaccaact cgatatggcg gtgatttatg
     8461 agcattcccc tgtggctggg gtatccagtc aggctttgct gaaagaagat cttttcctgg
     8521 taggaactca agattgcccg gggcaaaacg ttgatgttaa tgctattgcg caaatgaacc
     8581 tttttctccc cagtgattac agcgctgtta gacttcgtgt tgatgaggct ttttccctac
     8641 ggcggctcac ggcaaaagtc attggtgaaa ttgagtctat tgccacgctt accgcagcga
     8701 ttgccagcgg catgggcgtt gcagtattac ctgaatcggc tgcgcgttcg ttatgtggcg
     8761 cagtaaatgg gtggatgtca cgcattacca ctccttcaat gagtctctct ttgtccttaa
     8821 atttacccgc cagagcaaac ttgtcaccac aggcacaggc agtgaaagag ttgttaatgt
     8881 cagtgattag ttctccagtg atggaaaaaa ggcagtggca attggtgagc taagcgttat
     8941 atcgggatgg aataagatgc gggtttttat tatttgttat gccgggcatt agactttaac
     9001 aataacggga tatctgaact gcccggagtt taccgtgaat ttccaacaac taaagataat
     9061 ccgcgaggct gcacgtcagg attacaacct gacagaggtt gcgaatatgc tttttacctc
     9121 acagtcaggc gttagccgtc atattcggga actggaggat gaacttggca tcgaaatatt
     9181 tgttcgacga ggtaagcgac tgctggggat gactgaaccg ggcaaagcat tactggtcat
     9241 tgcagaacgt attctgaatg aagccagtaa tgttcgtcgg cttgcagacc tgtttaccaa
     9301 cgatacgtct ggtgttctca ctattgcaac gacgcatact caggcacgtt atagcttgcc
     9361 agaggtcatt aaagcttttc gcgaactttt cccggaggtt cggctcgagc taatccaggg
     9421 ttcgccacag gaaattgcga cgttgttgca aaatggcgaa gctgatattg gtatcgccag
     9481 cgagcgtttg agtaatgacc cgcagctcgt cgccttcccg tggttttgtt ggcaccatag
     9541 tttgcttgtt ccactcgatc atcccttaac gcaaattaca ccattgacgc tggaatcaat
     9601 agcgaagtgg ccgttaatta cttaccgaca gggaattacg gggcgctcac gcactgatga
     9661 cgcatttacc cgcaaaggtt tgccggcaga tattgtatta agtgcacagg attctgatgt
     9721 cattaaaacc tatgttgctc ttgggcttgg gatcggatta gttgccgaac aatccagcgg
     9781 cgaacaagag gaagagaatt taatccgcct ggatacgcgg catcttttcg atgctaatac
     9841 tgtctggttg ggactgaagc gaggacaact tcagcgtaac tatgtttggc gctttctgga
     9901 actttgtaat gcaggactgt cagttgagga tatcaagcgc caggtgatgg aaaacagtga
     9961 agaggaaatt gattatcaga tatagcaaaa agcccgctat aaaagcgggc ttttcagaaa
    10021 tttggctcct ctgactggac tcgaaccagt gacatacgga ttaacagtcc gccgttctac
    10081 cgactgaact acagaggaat cgctgttgag gcacatctta acggcgaaaa atattttgtc
    10141 aaacccaatt tttaaatttc ccaactgttt gccgtttcta tcaacagttt gttgcatttg
    10201 cagacaattt gttggcgaaa atctttgcag ctttgctgat tctccctcat catttgaaat
    10261 gtggtttcac tttctgcaat taaggtcggc tttgaatatc tcctctgctt tacgccaggt
    10321 tgttcacggc actcgctggc actctaaacg caagagctac aaagtgttgt tctggcgcga
    10381 gataaccccg cttgctgttc ctatcttcat ggagaacgcc tgtgtcctgt tgatgggggt
    10441 cctgagcact tttctggtca gctggctggg aaaagatgcg atggccggcg tggggttggc
    10501 ggacagcttc aatatggtca ttatggcttt ttttgcagct atcgatcttg gtactactgt
    10561 cgttgtagca tttagtctcg gtaagcggga tcgacgacga gcgagggtgg cgacgcggca
    10621 gtcattggtg atcatgacgt tgtttgccgt actgttggca acgctaattc atcattttgg
    10681 cgaacaaatt attgatttcg tcgcgggtga tgccacgaca gaagttaaag cactggcgtt
    10741 gacttatctg gagctgaccg tactcagtta tccagccgct gccatcactc ttattggtag
    10801 cggggcactt cgtggtgcag ggaatacgaa aataccgcta ctgattaacg gtagcctgaa
    10861 tattcttaat attattatta gcggcatatt gatttacggc cttttctcct ggtcggggct
    10921 gggttgggtt taaccatttc tcgttatatt ggcgcagttg caattttgtg ggtgctggcg
    10981 attggtttta atcctgcgct aaggatttcg ttaaagagct attttaaacc gctgaatttt
    11041 agcattatct gggaagtcat ggggattggt attccagcga gtgtcgaatc agtgttattt
    11101 accagtggtc ggttattaac ccaaatgttc gttgccggga tggggaccag cgttattgcc
    11161 ggaaatttta tcgcgttctc aattgcggct cttatcaact tacccggaag tgcgctcggt
    11221 tctgcttcta cgatcattac aggccgaagg ttgggggtag ggcagatagc gcaagcagag
    11281 attcagttgc ggcatgtgtt ctggctgtcc actcttggat taacggccat cgcctggcta
    11341 acggctccct ttgccggggt tatggcatcg ttttacaccc aggatccaca ggttaaacac
    11401 gtcgttgtga ttctgattta gctaaatgct ttatttatgc ctatttggtc cgcctcatgg
    11461 gtgctacccg ctggatttaa gggtgctcgt gatgcccgtt acgccatgtg ggtttctatg
    11521 ttgagcatgt ggggttgccg ggttgtagtc ggttatgtgc agggaatcat gcttggctgg
    11581 ggtgtggttg gtgtctggat gggaatgttt gcctactggg ctgtgcgggc cgtgctgttt
    11641 tactggcgaa tggttactgg acgttggctg tggaaatacc ctcgatccga gccgcaaaag
    11701 tgtgaaaaaa agccagctgt gtcggaataa acgacaaaat gcagattatt tcagcaaacg
    11761 atttcaaatt taaaaaacag gctttgacat tgtgggtggg catcgctaat attcgcctcg
    11821 ttctcacgat tcctctgtag ttcagtcggt agaacggcgg actgttaatc cgtatgtcac
    11881 tggttcgagt ccagtcagag gagccaaatt ttagttttga gacatctcta tgagactcag
    11941 gacagttaaa aaataaaaat tatttttacc cggccacttt gtaaaaaagt ggccgggttt
    12001 tttgatttct gtgtttcgtg cgcttaatcg agtctcacgc ttcagtgagc aaattgcccc
    12061 tccccaacag acatcacgat tcacaaggtc aaaaattctg ttccatgaag taccgtacaa
    12121 agataaagag aaaaatgcct tatgcgggat gtagctgtcg cgtctttatc gggaaactat
    12181 acgtaattgc cactttgtct tcggtaatca gaaaaagaag acctgccatt ttaataaata
    12241 ttatttctct gggaacgggg attttttata agcaatatga cgagttaaac attatatttt
    12301 ttataagaat tatatgagat taaagaaatc tgccataaag acagatttct ttaaaatata
    12361 attagagatt tgcgacgtta tgataaactt tttgtacatc gtcgtcatct tcaagggcat
    12421 caacaagccc ttcaaagatt tctaaatctt ctggggaaag ctcaacttca gattgagcaa
    12481 tcatttctaa ttctgttgtt gagaactcag tgattccagc tgcttttagc gccgcgattc
    12541 ctttatgaag gtcagcaggt tcagtataaa taacaatatt accttcttct tcagttacat
    12601 cacgaacatc aacttcagct tcaagcaaaa tttcaaaaat atggtcaggg tctgttcctt
    12661 taaatacaat cacacccgta ttgtcaaaca tatagctgac agaacctgcc gctccgatat
    12721 tgccgccttt tttattgaaa attgtgcgaa cgttagcaat cgtacggtta acatttgaag
    12781 tcaatgtctc agcaataatc attgagccat taggaccaaa gccttcataa cgtccctgta
    12841 cgaacgtttc atctccgccg cctttggctt tatcaattgc tttatcaata acgtgctttg
    12901 gaacttgtgc ctgctttgca cgttcaataa cgaattttaa agatgtgttt aattctggat
    12961 cgggttcacc ttgtttggca gcagcataga tttctacacc gaattttgca taaattttag
    13021 acgttgcacc gtctttagcc gtttttttag caacaatatt ggcccattta cgtcccactg
    13081 gaatgatctc ctcgaaaatt tatttacatt actggtaaat cgttaacatg tgttaattcg
    13141 gcatattata tcataacagg aaaaatctcg tctattagaa ataaaaatat tctaaagcca
    13201 taatcttata tttaatttta taaaaactaa tgttgttggc gggatttttt ataattgtgt
    13261 tttgaaagca tacttgcttt taatatgtgg aaatcattaa gacgataata aaactcgcat
    13321 ctgcccaagg aagcaagcag cccgcgttta ttgcaggcta tttgccagat gtcttactta
    13381 atccttcaca tctatttctg ataagaaggt tcataactta ttatcggaac ggcggctcat
    13441 taaaggttcg taatttacgt gaatgcagtc ggtcgccttc tgcgcgcagc aaatcgatcg
    13501 cccgaatgcc aatttgcagg tgttcggaaa tagcgccttc ataaaaacgg ttagcctggc
    13561 cgggaagctt aatctcacca tgtaacggtt tatctgaaac ac
//
LOCUS       DADMEQ010000102        13546 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.103, whole genome shotgun sequence.
ACCESSION   DADMEQ010000102 DADMEQ010000000
VERSION     DADMEQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.103"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(1..895)
                     /locus_tag="JNZ05_003032"
                     /pseudo
     CDS             complement(1..895)
                     /locus_tag="JNZ05_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1136..1936
                     /locus_tag="JNZ05_003033"
     CDS             1136..1936
                     /locus_tag="JNZ05_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY8566182.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2014..2784
                     /locus_tag="JNZ05_003034"
     CDS             2014..2784
                     /locus_tag="JNZ05_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY8566183.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2832..4190)
                     /gene="mltD"
                     /locus_tag="JNZ05_003035"
     CDS             complement(2832..4190)
                     /gene="mltD"
                     /locus_tag="JNZ05_003035"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HAY8566184.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4262..5017)
                     /gene="gloB"
                     /locus_tag="JNZ05_003036"
     CDS             complement(4262..5017)
                     /gene="gloB"
                     /locus_tag="JNZ05_003036"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HAY8566185.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5051..5773
                     /locus_tag="JNZ05_003037"
     CDS             5051..5773
                     /locus_tag="JNZ05_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY8566186.1"
                     /translation="MKPARVPQTVVAPDCWGDLPRGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5770..6237)
                     /gene="rnhA"
                     /locus_tag="JNZ05_003038"
     CDS             complement(5770..6237)
                     /gene="rnhA"
                     /locus_tag="JNZ05_003038"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HAY8566187.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6302..7033
                     /gene="dnaQ"
                     /locus_tag="JNZ05_003039"
     CDS             6302..7033
                     /gene="dnaQ"
                     /locus_tag="JNZ05_003039"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAY8566188.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7166..7242
                     /locus_tag="JNZ05_003040"
     tRNA            7166..7242
                     /locus_tag="JNZ05_003040"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7200..7202,aa:Asp,seq:gtc)
     gene            complement(7373..8419)
                     /locus_tag="JNZ05_003041"
     CDS             complement(7373..8419)
                     /locus_tag="JNZ05_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HAY8566189.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8430..9305)
                     /gene="tssG"
                     /locus_tag="JNZ05_003042"
     CDS             complement(8430..9305)
                     /gene="tssG"
                     /locus_tag="JNZ05_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HAY8566190.1"
                     /translation="MEKFRLGQTPALTFAPREIASVGWQEGRLHISLYSLGLWGPNGP
                     LPLHYTELARNRTESRRDPTLTRFSDLFHHRWLTQFYQAWRSAQSAGGGLDKPQHDRF
                     AFYVASLSGQDLRESADSPLPDHARLAASAHLVRESRNPDGLATTLAHYFGVPFRIQE
                     YVLHWIAVADDEITRLGMPAPSSVIGNGALIGQAVPDMQYKFRLVIGPLTLEDYRRFL
                     PGSNNLPVLTELVRAFSGYEYCWEIELQLKPHAAPPAVTGGPYQLGWTAWAGKAMHDN
                     PVTGMIFEPEHYLAH"
     gene            complement(9422..11305)
                     /gene="tssF"
                     /locus_tag="JNZ05_003043"
                     /pseudo
     CDS             complement(9422..11305)
                     /gene="tssF"
                     /locus_tag="JNZ05_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
     gene            complement(11321..11815)
                     /gene="tssE"
                     /locus_tag="JNZ05_003044"
                     /pseudo
     CDS             complement(11321..11815)
                     /gene="tssE"
                     /locus_tag="JNZ05_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
     gene            complement(11812..12642)
                     /locus_tag="JNZ05_003045"
     CDS             complement(11812..12642)
                     /locus_tag="JNZ05_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
                     /protein_id="HAY8566191.1"
                     /translation="MTNNNELPITLSALLRDYSVVEGIQMAEQQVRMHPAQASRRHSL
                     FQLLCVAGDWSRALQQIQLCARMDANYTREAQVFGELIRCEIYRHACFQGEQRPGVIL
                     PPPAWMEDLLTTLACNARGEAQEADAHRSRALEAITDTSGQWNGGAFDWISDSDSRTG
                     PVLELIAGGAYIWLPFSQICSLKSPRPAHLIDLIWKPVNVTLNNGDTHSAWLFPRYSG
                     SEKAADSLRLCRETVWQDGPGETLVQALGQRVWLTGHGDISLLDLSTCTFNTAEGSDA
                     "
     gene            complement(12629..13006)
                     /gene="tagK"
                     /locus_tag="JNZ05_003046"
     CDS             complement(12629..13006)
                     /gene="tagK"
                     /locus_tag="JNZ05_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
                     /protein_id="HAY8566192.1"
                     /translation="MLSPEQDLNRHADPFPFNEEPSSQDLDSLRDLVTEAETLQDMVT
                     GGLSIDAILNVLDATGEDETIWPVEKTPPDILHLLSPEYAPETAHNTVLPDLTRKEHR
                     IIGIDNHYRINPAQHGEIYHDKQ"
     gene            complement(13243..13413)
                     /locus_tag="JNZ05_003047"
     CDS             complement(13243..13413)
                     /locus_tag="JNZ05_003047"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HAY8566193.1"
                     /translation="MNDDVKIALTLTRHEEAWWIINQSTEYCCTVNDQIVEPHHRMRL
                     NEGDLIEWGLSS"
BASE COUNT         3101 a         3525 c         3617 g         3303 t
ORIGIN      
        1 ccgggagctg tttttacatg ctcgctaagg aaatcgataa aagcccggat gcgcgtactt
       61 accgcacggt cgctgtaata gacggcactg aatggcattt ccactggcaa cactttatct
      121 gccattaact ccaccaatta tccgcgagcg atttctttgt cgatcatgta gtcggacaaa
      181 cacgcaatcc cgttgccact caggcaaagc tgtttcagtg tttccccact attggatgac
      241 aaaccgtact tcacctcatg taattgtcca tcactacagg ctatcggcca ggtattgagg
      301 gaagcgggtt cagtgaatcc caggcaaaca tgttgcttta aatcgtcgat cgtttctggc
      361 ttcccgtagc gggaaatata atcgggggag gcgataattt ttcgataact gttaaataac
      421 ggcctggcac gtaagctgga gtccgttaac gtaccagcgc gtatcgcgac atccactttt
      481 ctttcgatca aattaataat cgtttcggag gagactagcg ataaagtgac ttccggatag
      541 cgttcacgga aaggcttaat taacggcatc agaaagtgca gcaccactgg agttgcggca
      601 tcgatccgta acagtccacg cggtgtatta cgcgtctcca taatttctga ttctgccgct
      661 gccatctcct gcaaaattga ctgtacgcga cgaaaataac gctcgccttc ttccgtcagg
      721 ctaagttgcc gcgtggtccg attaagcagg ctaacgccaa gtttcatttc cagctttttc
      781 accgcccggc ttaccgctga gtttgcttgc cctaaaattg ttccgctgcc cggctaaagc
      841 tgccgctttc gacgaccgaa acaaaaatgg cgagttcttc cgacgtggct ttcatttttg
      901 ctcctgttgc aaaatagatg agatattttg aatttatttg tcattaaacc atcaggatgc
      961 gtgatatgtc atccgattta atgttctcaa taatgagcaa atttctgacc ggtgtaagca
     1021 cttgcttaca taacaatata caattgctcg ttgaaagagt gagctaaaat ccctataaca
     1081 gtagaaccct cccgagtgcg gaagggttga cgtaatagag gtttcaaagt caaaagtgcg
     1141 aaaaaacacc tatgccatgc gctatgttgc cggacaacct gcggaaagga tcttaccgcc
     1201 ggggtctttt gcgagcatcg gccaggcatt accacctgga gaaccgttaa gtaccgaaga
     1261 gcgtattcgg atcctggtgt ggaacatata caaacagcaa cgcgctgaat ggttgtcggt
     1321 attaaagaac tacggcaaag atgcacatct ggtgttattg caggaagcgc agacaacgcc
     1381 agagttagta cagtttgcga ccgctaacta tcttgccgcc gatcaggtgc ccgctttcgt
     1441 gctgccacaa catccttccg gtgtaatgac cctttcggcg gcgcatccag tgtattgctg
     1501 cccgttacgt gaacgagaac ccattttgcg tctggcgaag tcggcactgg tgacggtcta
     1561 tccattacct gacacccgcc tgttgatggt ggttaatata cacgccgtca acttcagtct
     1621 gggcgtggat gtctatagta agcagttact tcctattggt gatcagatag ctcaccacag
     1681 cggcccggtc attatggcgg gagatttcaa tgcctggagc cgtagaagga tgaacgcgtt
     1741 atatcgcttt gcgcgggaaa tgtcgctgcg ccaggtgcgt tttaccgatg atcagcgccg
     1801 tcgggcgttt ggccgcccgc tcgattttgt tttctaccgt ggtctgaacg tcagcgaagc
     1861 ttctgtattg gttacgcgcg cttccgatca caatccgcta ctcgttgaat tcagtcccgg
     1921 caagcctgat aaataaggta tgtcaggtct gccacagggc agaccaacgt ttggcgctgt
     1981 ccttttatac tcaaacaaca aaaggacagc gcaatgacaa cacaatccca ccatgaccac
     2041 gtagaaaagc agtttagctc tcaagcctgc gaatatctga ccagtaccgt acatgcatcc
     2101 ggacgagatt tacagcgcct ggcggtgcgt ctggctgatt atcctggtgc aagtgtactt
     2161 gatatgggct gcggagcagg gcatgccagc tttgttgctg cgcaaaacgt gagcgcggtg
     2221 gtggcgtatg acttatctgc ccaaatgctg gatgtcgtgg cacaagctgc cgaagcccgg
     2281 caactgaaaa atatcaccac ccgccaggga tatgccgaaa gtctgccatt tgccgataac
     2341 gcatttgata ttgttatcag ccgttattct gcccatcact ggcatgatgt tggtgcagca
     2401 ctgcgtgaag tgaaccggat attgaaacct ggcggtagga tgattgtgat ggacgtaatg
     2461 tctccgggtc atccagtgcg cgacatctgg ttacagacgg tagaagcatt acgcgatacc
     2521 tctcacgtac gaaactacgc cagcggtgag tggttgaggt taatcaatga agccaatctg
     2581 atagttgata atttaattac agataagtta ccgctggaat tttgttcatg ggtcgcgaga
     2641 atgcgtacgc cagaagcgtt agtagacgct attcgcattt accaacagag cgcatcgaca
     2701 gaggtgagaa cgtattttgc cttgcagaat gacggctctt tcaccagtga tatcatcatg
     2761 gtagaagcac ataaagcggc ataaataaaa aaggcaccgg gggaatcggt acctttttat
     2821 tatctggttt gtcaggaatc tggcatgttg ttgtttttca caaacaacgt cagcttgtcg
     2881 cctggttgca gattcgcagt gtcgctgttc cagcgcatca catctttgat gttcacgccg
     2941 tggcgtttag caatgcttga aagcgaatcg cctttgcgca cacgatacgt aatgctatcg
     3001 ctgttgtttg ccagtcgctg tgcgctactg cctgcaccaa tcgtcaaact ttggcctggc
     3061 ttcagcttag atccgcgcag tttgttccac tgctgcaaat ctttggtgct tacgccgaga
     3121 cgtgaagcga tgcttgaaag cgtgtcgcca gagcgtacgg tgtaaacacg gctgttaagc
     3181 ggcgtattgt cggcaaccag cgtcgactgt acagcagcaa tttcgcctga agcgagagat
     3241 tcacgcagtt gatctgcatg cttctttggc accatcacgt actgcggccc acttgcgccc
     3301 agcgtggagc ctttcacgcc agcgttgaat gtcttcagct tgctgacgga aatccccgcc
     3361 atatctgcaa ccttcgccat ttcaaccggg ctactcaggt gcacacgcgc cagagcacgg
     3421 ctttcatcgg tcgttggcag acgtacgcca taacgcttgc tgtttttgag aatatcactc
     3481 aatgccagca ttttaggcac gtactgcttc gtttcctgcg gcaacggtaa cgaccagaag
     3541 tccgtggatt tcccacgcgc tttgttcgtt ttaattgcct tcatgacccg accttcgccg
     3601 ctgttataag ccgctacggt cagaagccag tcgccgtcaa acattttgtt cagacgctgc
     3661 atcatattca gcgcggcagt tgttgaagca acaacatcgc gacgcgcgtc ataattgcgg
     3721 gtctgtttca aaccataatt gcgccccgtg ctcggaatga tctgccagat gcctgcggca
     3781 ttggcgccag acgttgcgtg aggatcaaaa gcgctctcca ctatgggtag tagtaccagt
     3841 tccataggca tgttacgttt tttaacttgc cctgctatcc agtacatata cggctctgcc
     3901 cgtaaagtta catcgtggag atagctctta ttgcgtaaat atttctgttt ctgttcgcga
     3961 atccggtcat tttccggaat tcccatcttt agctcgtcgc caatgaaagc ccacaagtca
     4021 ccatctggcg cgatagacgt cccatcgtcc atccatcgtg cctgacttgt aaactttgct
     4081 gcttcccctt gaccagctgc agaaaggctc tgtgcgtgct gttgaacgtt gccggtactc
     4141 tggcaaccca cgagcaggac agaggcgagt aatatcgctt ttgccttcat gtgtgtgtca
     4201 atagttgctt aaaagacgac cgatcataac ggcgaacgga gccgatgaca agaaagtttt
     4261 atcagaacct atctttcttt gaccttaacc atgcaaaacg ctcttcaggt tgttgcaata
     4321 atgtttcttc attaattaca ttaattaaat caatatcttc cgttcttaaa aaaacattaa
     4381 tttgccgctc atttttcaga attacgggta gtgttatttg attttttgcc cgtaactcct
     4441 taactttacg ataataatca tttatggaca aatcgtgcgg aaaaatactc aaagcaaact
     4501 tcatatttga taacgtatat tcatgagcac aacataccaa tgtatcgtca ggtaacgcac
     4561 ttagcttttt aagtgattga tacatttgta aagctgtccc ttcaaacaac cgaccacacc
     4621 caccagaaaa cagcgtgtcg ccgcaaaata gataaggttt actgaagtaa cagatatgtc
     4681 ctaaagtgtg acccggtgtg gtaattacac taaattcatg ccctaaaacg aaggcagttt
     4741 cgccatcttt gactacctgt gttgttccct tatcttgtgt ctcttgtgga ccatacacca
     4801 caatttgcgg aaacttttcc accagttctt ttacgccgcc aacgtgatcg tggtgatggt
     4861 gggttagaaa tatggcctcc ggttgccagt tattggcggt aatggcgttt aatactggct
     4921 ctgcgtctcc gggatcgaca atcaggcagc gacctgcctc atcattcaaa acccagatgt
     4981 aattgtcatc aaaggcggga atactgttaa gattcataga ttacctctca gtgtaaaacg
     5041 gaaggttgtg atgaaaccgg caagagtccc tcaaactgtc gtggctcctg attgctgggg
     5101 cgatttgccc aggggagagc tttatcgcaa ggcgctggag cgccagctca acccgtggtt
     5161 cactaaaatg tatggttttc atctgcttaa gattggcaat ttaagcgcag aaatcaattg
     5221 cgaagcgtgc gcggtttctc atcaagtgaa tgtttctgcg caaggaatgc ccgtccaggt
     5281 gcaggcggat ccacttcatc ttccttttgc cgataaatcc gttgatattt gtctactggc
     5341 acataccttg ccgtggtgca ccgatccgca tcgtttatta cgtgaagccg atcgggtact
     5401 gattgatgat ggctggctgg tgattagtgg cttcaacccc atcagtttaa tgggattacg
     5461 caaacttgtg ccggtattgc gcaaaacctc gccctataat agccggatgt ttactctgat
     5521 gcggcaactg gactggctct ctttgttgaa ttttgaagtg ctgcacgcca gccgtttcca
     5581 cgttctcccg tggaacaaac acggaggaaa actattgaat gcgcatattc ctgcgcttgg
     5641 ttgcttacaa cttattgttg cccggaaacg gactattcct ttaacgctaa atccgatgaa
     5701 acagagtaaa aacaagccac gaattcgcca ggcggttgga gccacccggc aatgtcgtaa
     5761 accacaggct taaacttcga cttggtagcc cgtatcttcc agtgtgggat tcatcgccgc
     5821 ggcacgagcc agttcatcac agcgttcgtt ttccgggtgt ccggcatggc ctttaaccca
     5881 ttcccatttg atttgatgct gccccaatgc agcatcaaga cgttgccaga gatcgacatt
     5941 ttttactggt tttttgtctg ctgttttcca gccacgtttt ttccagttat ggatccactg
     6001 ggtgataccc tggcggacat actggctgtc ggtactcaaa atgacttcgc aatgttcttt
     6061 taacgcctcc agcgcgacaa tagcggccat caactccata cggttgttgg tggtgcgggt
     6121 gtagcctgcg ctaaaggttt tctcgcgtcc gcgatagcgt aaaatagcgc cgtaaccccc
     6181 aggtccagga ttacccagac acgaaccatc ggtgaaaatt tctacctgtt taagcatctc
     6241 tggtagactt cctgtaattg aatcgaactg taaaacgaca agtctgacat aaatgaccgc
     6301 tatgagcact gcaattacac gccagatcgt tctcgatacc gaaaccaccg gtatgaacca
     6361 gattggtgca cactatgaag gccacaagat cattgagatt ggtgctgttg aagtagtgaa
     6421 ccgtcgcctg acgggcaata acttccatgt ttatctcaaa cccgatcggc tggtggatcc
     6481 ggaagccttt ggcgtacatg gtattgctga tgaatttttg ctcgataagc ccacgtttgc
     6541 cgaagtagcc gatgagttta tggactatat ccgcggcgcg gagttggtga tccataacgc
     6601 agcgttcgat ataggcttta tggactacga gttttcgttg cttaagcgcg atattccgaa
     6661 gaccaatacc ttctgtaagg tcaccgatag ccttgcggtg gcgaggaaaa tgtttcccgg
     6721 taaacgcaac agcctcgatg cgctatgtgc ccgctacgaa atagataaca gtaaacgaac
     6781 gctgcacggg gcattactcg atgcccagat ccttgcggaa gtttatctgg cgatgaccgg
     6841 tggtcaaacg tcgatggctt ttgcgatgga aggagagaca cagcagcaac aaggtgaagc
     6901 aacaattcag cgcattgtac gtcaggcaag taagttacgc gttgtttttg cgacagatga
     6961 agagctggca gcacatgaag cccgtctcga tctggtgcag aagaaaggcg gaagctgcct
     7021 ctggcgggca taaatacatg taaaaggcgc taaaaatagc gacttgggcg atttttgcag
     7081 caaacgattc aaaagatgag aaaaatcgtt gacgaaggtc gaggcaatcc gtaatattcg
     7141 cctcgttccc aacggaacac aacgcggagc ggtagttcag tcggttagaa tacctgcctg
     7201 tcacgcaggg gggcgcgggt tcgagtcccg tccgttccgc caatattcag aaagcccgaa
     7261 tcagtaatga ttcgggcttt cgtcgttttg gtgaagtgag aatttttcac tccgaagtaa
     7321 aacggatatg atttaaaaaa tggccagaca ttataaattc tggccacttc tgttactcca
     7381 gcgcatccca tttctccagc agttctactg gaatttcatt tattacctcc gagacgcgtt
     7441 ttccgaccag gcgttctgcc tggcgcagca aaggaatggt cgggctgctc ggttcgctgt
     7501 gctcaaacca gcgacggatg acacgcaggc gttccagcgc gtcgtttcgg tcgcggatct
     7561 gccggggttc tgcgccggtc atcggtgata tttcctccgc gatatgcacc ggtagcgcaa
     7621 tctgctctgc ctgcatctgt tcagtatgaa ctatggtggc gttttccggc tgctcagcct
     7681 gtggttgtgg caggatctcc ggcgtggtgc tctgcactgc tcccggtaac agggccagca
     7741 gttgcgtcag acgggagaag tccggtgcct ggtcattcag cgtttcacgc gcatggcgtt
     7801 gcagccgctc agtggtctct gctgcctggc gaaaggcatc cagcggcagt gtgccgcgcg
     7861 cttccaggtc tgccagttgc tggcgtaccg attctggtgc cagcgcatcc gccgggcgta
     7921 atgcagatag cgaacgctcc acatcccgca cctgaaggcg catggcagca ttatttgaca
     7981 gtgtaatgcc acgaatatcc gccattacac cttcgtggtc cagcaacgcc gccagcgcgt
     8041 tactgcgtgc caccgcagca tcttcggcgg tgatatcttc agtcgcgagc agttgaggat
     8101 ggatggcgtc cgggtacgtg gagcacaacg tttccagcag cgtgagcgcc tccgacagtc
     8161 ccctggcacc ggcctgctgg atacggcagc gcagcaggac aaccagaatg cggatatctt
     8221 tgctgcgcat cagcagacga tgggcgtccc gctcaagctc cgcccagtta atgttttccg
     8281 gcgtactgac aaaatcgcca tattgcgctt cttcacgcgg tgcggcgcgg gtgaacagca
     8341 gcaggtattc cggatcgtac tccagatccg ggccgcaggg gcattccgcc gaaattgggt
     8401 cagacaggaa tatttccatt taattatcct cagtgcgcca ggtagtgttc tggttcgaaa
     8461 atcatgcccg tcaccgggtt gtcgtgcatg gctttacccg cccatgcagt ccaacccagt
     8521 tggtacggac caccggtaac agccggaggg gcggcgtggg gctttagctg tagctcaatt
     8581 tcccagcagt attcataacc actaaaggcg cggaccagtt ctgtcaggac gggcaggtta
     8641 ttgctgccag gcagaaaacg tcggtaatcc tccagcgtca gcgggccgat aaccagccgg
     8701 aacttatact gcatgtccgg cacggcctgg ccgatgagtg caccattgcc aataacggaa
     8761 gagggggcag gcatccccag ccgggtgatc tcatcgtccg ccacggctat ccagtgcagg
     8821 acatactcct gtatccggaa aggtacaccg aaatagtgcg ccagcgtggt ggccagaccg
     8881 tccggattgc gggactcacg caccagatgg gccgatgccg ccagacgagc gtgatctggc
     8941 aacgggctgt cagcgctctc ccgcaaatcc tgcccgctga ggctggcgac ataaaaggca
     9001 aagcggtcat gttgcggttt atccagaccg cccccagcgg actgggcgct gcgccatgcc
     9061 tggtaaaact gcgtcagcca gcggtgatga aaaagatcag aaaaacgggt cagcgtcgga
     9121 tcgcgtcggc tttcagtccg gttacgcgcc agttcggtgt agtgcagcgg cagtggaccg
     9181 ttcggccccc acagcccgag gctgtacaga ctgatgtgca ggcgcccttc ctgccagccc
     9241 acactggcga tttctcgtgg cgcaaaggtc agcgcgggcg tttgtccgag gcgaaatttt
     9301 tccatccgtg gttgctaggt gttgcccgcc ggaacggtac acaactgcac atccacgcgg
     9361 cgcatcaggt tcagaaaacc gtaatgccag gggggttcct gcgcctgagt gaaatcgttg
     9421 ctcatacgct tccccgctgt cccgccctga caggccaggt cataattttg ccgcgctgca
     9481 tgctgtgcag cgtcatctgc gagaaggtat taatggaaac atggcgggcg atatagtgtt
     9541 ccagcaccag gccgaacaga tacggactga tgccggaaaa accttcttca tccaccgtca
     9601 gttcacaact gacgccacgc ccatagacca gtagtccgga accgggcaga cggcgggtga
     9661 ccggggtcgt tttacaacca atcaggctgc gcacctggcg cgattgcgga ctgtcatgag
     9721 caggaataaa caggttcagc agatctcgca acgcctggcc gccggtacgg tgatccagat
     9781 cggccagcgg cagataatta aaagataact ggcggatcaa ccgccatgcc atctcacgtt
     9841 cagccagcgg gggctgcggc gagcgtggcg ggcggataag ccctacgccc gccaccggga
     9901 tggctgcatc cacggttaga tcgtcccggc cattgcgggc aataagacag ggcagatcgc
     9961 ggttagtgac catggccgtg atggtgatgt ggcgcagatt ttccgggtac ggcgcttcat
    10021 actggtcaac cagcgacagg aacacttccg agcccgtata gggggtacgg gtgccgtaac
    10081 ggcgggcgtt ttctgataag cggcggggct cccggcgcag ggaaaaataa cgtccgtgat
    10141 tgccttcatc attattgcgg gtgtggtaga gcgggcgaaa ggccatcttc cgtgtggtgt
    10201 cggtttccag cccttccact tcctggaccg agaacacctc ataatccagc ggatgggttc
    10261 tgtccaccac cagatgctgt tccgtcgtgc tgtgcgtgac atcgatccgg gcggtgacgc
    10321 ggggaaacag gttgataacc ggggtacaga acaggctgaa ctgtgccgca tttgtctggt
    10381 ggatcagtca gtccggcggc aggcggttca gcaggataac aatctccgcc acgccaccgt
    10441 caattttctg taatccggca gacaggcctg tcggggtgaa aaaatagaag cgttccggac
    10501 aggcaaaata ttcatgcagc aggttatgac cgtgaaacac gttccacgcc agcggcagca
    10561 gcccctgatc cggctccagg ccttcgtaca taacgggctg ttgcagattc acatccagcg
    10621 cgccgtcaaa atgccccgga atgccagcca gggtcgcgat ggcgctggta tgtagaagtt
    10681 ctaacaggtg tgaggccgta cgctcctcgc cgcagagata aaacggcagc ctgtccagtc
    10741 ctgcaagctg actgaacgtt agttcaccaa aggtgcgcag ggtaatacgc agcgccccgg
    10801 cgacgtgaat gttggccggc aggtagcggt gcagggcagg catatccggt ggcgcggcgg
    10861 tcagtcgcgc ctcttcgatg gcgagcggcc acagcgttac atcctggctg ctgcgaaact
    10921 ggcaggcagt gctttcgcct tccggaatgg cggagaaaaa ggctgtgtcg cgcgggacgg
    10981 taaatccttt cgccagatcg ccttcttccg tatcgggatg caactgcgcc acgctcattg
    11041 agggcgtggg tgtgacgtaa ttggggctga ccacttccag cgggcgctgg gtgaaacggg
    11101 ggaattcagc gtcaattttt agctgcgtgc gggcggtcag gaagctgaag gcctcgacca
    11161 ttcgctctgc atacggatcg gcaatatccg ttccctgcat gcccagccgg gcggcgactt
    11221 tgggatgacg ggcggcaaat tcggctcccg tttcccgcag atagctcagt tcgcggttgt
    11281 agtatcccag tagccgtgga tccatcggtc tctctctgaa ttaaaaaaac gtaatgcgac
    11341 tctgctccat atccagcgcg ctacgtaccc ggaatgcagt cggatacggc tgcgtgagga
    11401 tctcgccgcg aatttcaaac tgtaatgtgt tatggccact ctggcggcgc tcatcctgaa
    11461 gtgggatgat ttgcagggtg gacgctttca gccggggttc aaaccgggtg atggttcgca
    11521 aaatggccct gcggatatcg tcccacttgt gctcatacag aaagctgccc gccagtggcg
    11581 gcaggccgta gttaagtacg gacgctgccg cgtacggata acgtgcagca tcaatatcat
    11641 cttcatggct gacggtgttg agcagaaatg actaatcccg gcggatgatc tccttaatct
    11701 gaaccgggct gacgctgata ccgtggtcgc gtttctggtt tggggcgtcg tcacacaggc
    11761 ggtcaaacag cgtgggcagc aggtgattgc ctggacgctg gcgggaagcg ctcacgcgtc
    11821 gcttccttca gcggtgttaa aggtacaggt ggacaaatcc agcaggctga tatcgccgtg
    11881 accggtgagc cacaccctct gacccagcgc ctgtaccagt gtctctcccg gaccgtcctg
    11941 ccagaccgtt tcccggcaca gacgcaggga gtcggcagcc ttttcagagc cgctgtagcg
    12001 gggaaacagc caggcactgt gtgtatcgcc attgttcagg gtgacgttga cgggtttcca
    12061 gatcaaatcg atcagatgcg caggccgcgg cgatttcagc gaacagattt gtgaaaacgg
    12121 cagccagata tatgctccgc ccgcgatcag ttcgagtacc ggaccggtac gggagtcgct
    12181 gtcgctaatc cagtcaaatg cgccgccatt ccactgcccg gaagtgtctg ttatggcctc
    12241 aagcgcgcgg ctgcggtgag catcagcttc ctgcgcttca ccacgggcgt tacaggcgag
    12301 ggtggtcagc agatcctcca tccaggcggg cggcgggaga ataacaccgg gccgctgttc
    12361 gccctgaaag caggcgtggc ggtatatctc acagcggatc agctcgccaa atacctgcgc
    12421 ttcgcgagta taatttgcgt ccatccgggc gcatagctgg atctgttgca gtgcccgcga
    12481 ccagtcgcct gccacgcaca ataactgaaa caggctgtgg cggcgggagg cctgcgctgg
    12541 gtgcatcctg acctgttgct ctgccatctg aatgccctcc accacggaat aatcccgcag
    12601 cagcgccgac aaggtaatcg gtagctcgtt attgtttgtc atgatatatc tctccatgtt
    12661 gtgccgggtt tatccggtaa tggttgtcaa tgccaatgat gcgatgctct ttccgtgtca
    12721 gatctggcag tacggtgtta tgcgctgttt ccggggcgta ttccggggaa agtaaatgca
    12781 ggatatcagg gggagtcttt tcgacaggcc agatagtttc atcttcgcca gtggcatcca
    12841 gtacattgag aatagcgtca atgcttaacc caccagtgac catatcctgc aatgtctcgg
    12901 cttcggtgac caggtcacgc aatgaatcca gatcctgcga ggagggctct tcgttaaaag
    12961 gaaaaggatc agcatggcga ttaagatctt gttcgggaga taacaacgcc tccggtattc
    13021 ctgataaagt cgggtcaggg catttccttt ttccgcacct tctccggtgg aggaggctcc
    13081 atgaacagga atgatatcga atggattttg tgatgtcaga tgctgctgcc tgaaccaatc
    13141 cagatccaga tattcggtta ttgcttccga cgtgatggca gtctgcgata tcgtttttac
    13201 tgtgctcagg tccggagatg atggattgcc atgtacaagg cactatgaag ataaccccca
    13261 ttctatcagg tcgccttcat tcagacgcat acggtgatga ggttccacaa tctggtcgtt
    13321 taccgtacag caatattcgg tagactgatt gataatccac catgcttctt cgtggcgggt
    13381 cagagtgagg gcaatcttta catcatcatt caccttaaaa acaggaatcc agggagaggt
    13441 aacggtgaag ataatttgtg ttccttcagt cgcgtaaatt gtgttatcgg cgatcccgtg
    13501 cgcctgtatt ttttggagtc cccacgtaat atttggtttc attgtg
//
LOCUS       DADMEQ010000103        13453 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.104, whole genome shotgun sequence.
ACCESSION   DADMEQ010000103 DADMEQ010000000
VERSION     DADMEQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13453
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.104"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            33..563
                     /locus_tag="JNZ05_003048"
     CDS             33..563
                     /locus_tag="JNZ05_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY8566194.1"
                     /translation="MSITGVNIGRLFREGFSNYDPIGVLNAMASQRTKEARGGELQIN
                     ELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGAAKMLFSHAQKTLAWDAFAFT
                     EVLLTQPVMVVVGEKVGAFGVYRDGLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAV
                     QEALAKVIPFFNTHLG"
     gene            581..1219
                     /locus_tag="JNZ05_003049"
                     /pseudo
     CDS             581..1219
                     /locus_tag="JNZ05_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            1510..2238
                     /gene="mpaA"
                     /locus_tag="JNZ05_003050"
     CDS             1510..2238
                     /gene="mpaA"
                     /locus_tag="JNZ05_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HAY8566195.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2213..3178)
                     /gene="ycjG"
                     /locus_tag="JNZ05_003051"
     CDS             complement(2213..3178)
                     /gene="ycjG"
                     /locus_tag="JNZ05_003051"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HAY8566196.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3297..3803
                     /gene="tpx"
                     /locus_tag="JNZ05_003052"
     CDS             3297..3803
                     /gene="tpx"
                     /locus_tag="JNZ05_003052"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HAY8566197.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGLLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3847..5388)
                     /gene="tyrR"
                     /locus_tag="JNZ05_003053"
     CDS             complement(3847..5388)
                     /gene="tyrR"
                     /locus_tag="JNZ05_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HAY8566198.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5536..6597)
                     /locus_tag="JNZ05_003054"
     CDS             complement(5536..6597)
                     /locus_tag="JNZ05_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HAY8566199.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6594..7991)
                     /locus_tag="JNZ05_003055"
     CDS             complement(6594..7991)
                     /locus_tag="JNZ05_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HAY8566200.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQTTTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8146..9144
                     /locus_tag="JNZ05_003056"
     CDS             8146..9144
                     /locus_tag="JNZ05_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY8566201.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKKSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9255..10160)
                     /gene="ompG"
                     /locus_tag="JNZ05_003057"
     CDS             complement(9255..10160)
                     /gene="ompG"
                     /locus_tag="JNZ05_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HAY8566202.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(10205..11287)
                     /locus_tag="JNZ05_003058"
     CDS             complement(10205..11287)
                     /locus_tag="JNZ05_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8566203.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTILEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(11301..11960)
                     /gene="pgmB"
                     /locus_tag="JNZ05_003059"
     CDS             complement(11301..11960)
                     /gene="pgmB"
                     /locus_tag="JNZ05_003059"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAY8566204.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11957..13453)
                     /locus_tag="JNZ05_003060"
     CDS             complement(11957..13453)
                     /locus_tag="JNZ05_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAY8566205.1"
                     /translation="MCAQQSYDQHLAASTENWRQWWQKRRITVNGGDAHDQQALDYAL
                     YHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWDTEVFLLPFHLFSDPTVARSLLRYRW
                     HNLPGAQEKARRNGWQGALFPWESARSGEEETPEFAAINIRTGLRQKVASAQAEHHLV
                     ADIAWAVIQYWQTTGDESFIAHEGMALLLETAKFWISRAVRVNDRLEIHDVIGPDEYT
                     EHVNNNAFTSYMVYYNVQQALSIARQFGCSDDAFIHRAEMFLKELRLPEIQPDGVLPQ
                     DDSFMTKPAINLAKYKAAAGKQTILLDYSRAEVNEMQILKQADVVMLNYMLPEQFSSA
                     SCLANLQFYEPRTIHDSSLSKAIHGIVAARCGLLTQSYQFWREGTEIDLGADPHSCDD
                     GIHAAATGAIWLGAIQGFAGVSVRDGELHLNPALPEQWQQLSFPLFWQGCELQVTLDA
                     QRIAIRTSAPVSLRLNGQLISVAEESVFCLGDFILPFNGTATTHQEDE"
BASE COUNT         3334 a         3646 c         3211 g         3262 t
ORIGIN      
        1 gccgataaag acctaaacga attaaagctg tagtgagtat tacgggtgtt aatatcgggc
       61 gtctttttcg ggaaggtttc agcaattatg atccgattgg cgtattgaat gcaatggcgt
      121 cacaacgaac aaaagaggct cgtggtggag aattacagat caacgaatta cttcccgcca
      181 gcctggatgc agcaaaagcc catggactga ccgaacgcga tgtttatgaa gcaactgact
      241 actataaaac accacgcggg caacaaccgg gaggtgcggc aaaaatgttg ttctcacatg
      301 cacagaaaac gctggcctgg gatgccttcg catttactga agtgttactg acccagccgg
      361 tgatggtagt agttggtgaa aaagtcggtg ccttcggtgt ttatcgtgat ggactggaag
      421 tctatggccg ggcgatggtt tcgcaggatc gtcagttagt ttcgttaccc gatttctcac
      481 attatgaact atatgacaaa ccggaagcag ttcaggaggc gctggcaaaa gttattcctt
      541 ttttcaacac ccatctggga taagtttcat gagaaatatt atgaaaaatg tactgattct
      601 tggtgctggc ggtcagatag cccgccatgt gattaatcaa ctggcagata aacaaactat
      661 taaacaaact ttatttgccc gtcagccagc aaaaatacac aaaccctatc caactaatag
      721 ccagattatt atgggggatg tactgaatca tgcagcactg aagcaggcca tgcagggaca
      781 ggatattgtg tatgccaacc ttacggggga agatttagat attcaggcaa attcagtgat
      841 cgctgcgatg aaagcctgtg atgttaaacg tctgatcttt gttctttccc tcggtattta
      901 tgatgaagtt ccaggaaaat ttgtcgaatg gaataatgca gttattggtg aacccctaaa
      961 accattccgc agggctgcgg atgcaattga ggcttctgga ctggaatata caattcttcg
     1021 ccctgcctag ctgactgatg aagatattat tgattatgag cttacctctc gcaatgaacc
     1081 attcaaaggt acgatcgtat cacgtaagag tgtcgcagca ttaattacag atatcatcga
     1141 taaacctgag aaacacattg gtgagaatat aggcatcaat caacccggga ctgatggcga
     1201 taagccattc ttcatgtaaa tcaattatat agataccatc gtactgtact atgtgcagta
     1261 cgatttatta aacttattaa ggtgaactga tcaatcagat gcgattaaca tgtgtagaac
     1321 cagtaagaat aaaatacgcc atgtatataa cattacgaca attctaatat ctgcacatca
     1381 atgaaaatgc aaacaacttc cggcaaaaaa gtgatacaag atcgatgaca tagcgttgaa
     1441 gaaaaggatt tgataatccg caaaatttgc acaacactgc ctgtaatact tcctgaacca
     1501 gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg gcgcatttcc gcccggaaca
     1561 gaacattacg gacgttcatt attgggcgcg ccgttaatct ggtttcctgc ccctgcagcc
     1621 agccgcgaaa gtggtttgat tctggctggc actcacggtg atgaaaactc ttcggtcgtc
     1681 acgctctcct gtgctctgcg gactttgaca ccttctttgc gccgccatca tgtggtgctg
     1741 tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca atgctaatgg tgtggattta
     1801 aacagaaact ttccggcggc gaactggaag gaaggtgaaa cggtttatcg ctggaacagc
     1861 gccgctgaag aacgtgatgt cgtattactg acaggtgaca aacctggctc cgaacctgaa
     1921 acccaggcgc tgtgccagct tatacaccgc attcaacctg cctgggtggt ctcattccac
     1981 gatccactgg cttgtattga agatcccaga cacagcgaat taggcgaatg gctggcccag
     2041 tcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa cgcctggctc tttcggcagt
     2101 tggtgtgcag atctcaactt gcattgcatc accgcagagt ttccgcccat ctcctccgac
     2161 gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc tgcgctggca tcctaaagat
     2221 gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc taccgccagc caggtcggtc
     2281 cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa agcggcgcta atggcacgag
     2341 aggtacacaa catgcagccc agcatcagac tgaaaccttg tgcacgcgct tcagtcgcca
     2401 gcgccagcgc ttccgtcaga ccaccggttt tatcgagctt aatgttaacc atctcatagc
     2461 gccctttcag cgccttcaga ttgctacgag tatgacaact ttcatcagca caaatcggca
     2521 acggatgaat aaaattctcc agcgccgcat cgtcctgcgc aggaagcggt tgttcaagca
     2581 tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc caacccttct gcacgccagg
     2641 attcatttgc atcaacgatc agcgtggcat cgggcacagc ggtgcgaatt gccaccatcc
     2701 gctcactgat aagatggtta tccagcttca ctttcagtaa tttcgcgcct gcctgccaga
     2761 gtgttgatgc actattggcc atctgatcag gcgtaccgat aacaaccgtc tgtgcagtga
     2821 taactgtctc gggaagcgtt atgccgatca aatcagccag cgattgctgc tgttttcgcg
     2881 ccgcgagatc ccacaatgca caatccagcg cattacgtgc tgcgccggca gggagaattt
     2941 tttgcaactc ctcccgtgtc agccctttct ctaattgcgg cacgacgctc ataatttgcg
     3001 ccattaccga ggcatcactt tctccataac gcggatacgg cgtgcattcg ccggtgcctt
     3061 taataccctc ttcttccagt tcaaccacca ccacgcgcgc ttcactgcgg cttccccggg
     3121 caatcacaaa cggggtatgt aagggccagg cttcctcaaa taccttaacg attctcatct
     3181 ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa atagcgtttg ctgtgttatt
     3241 gacagttagc ataaactagg tgtgacgtta actatatgta aacaggaaag ataatcatgt
     3301 cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc caattccatc ccgcaggcgg
     3361 gtagcaaagc gcagactttt actctcgtgg caaaagatct gtctgacgtc accctcggtc
     3421 agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag tattgatacc ggtgtttgcg
     3481 ccgcatcagt acgtaagttt aaccaactgg caactgagat cgacaacacc gttgtgctgt
     3541 gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg cggcgcagaa ggtctgaaca
     3601 acgttatcac cctctccact ttccgtaacg ctgaatttct gcaagcctac ggtgtggcaa
     3661 ttgctgatgg cctactgaaa ggtctggcag cgcgtgccgt tgtggttatt gacgaaaatg
     3721 acaatgtgat tttcagccag ctggtggatg aaatcaccac cgagccggat tacgaagcag
     3781 ctctggctgt actgaaagca taagttaata tgcctgatgg tgttgcacca tcaggcatat
     3841 tcgcgcttac tcttcgttct tcttctgact cagaccatat tcccgcaact tattggcaat
     3901 cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc gtgctgggat aattgcgata
     3961 aagctgggtt aataccgagc gttcaaaacg gctggtgatt tcgtccagcg aaccttccat
     4021 cgcatcttcg cccacggcta ccgttgctgc gtcataatcc ggcaacaaaa tatcctgtgg
     4081 acgcagctca taaccgtcca gttgtgtcag tgcgcgatag atagcgttct ttaactgccg
     4141 cacatttccc ggccacgcat aacgtgtaag tacagtattc aggtcagcgg ccagtttcgg
     4201 acgcggcacg ccctgctcgt cggcaaagcg ggcgacgaac agctcagtta acggcataat
     4261 gtcctgcgga cagtcacgta gcggcggcag attgagcgtc aacacgttca gacgataata
     4321 gagatcttca cggaacacgc ctttttgcac cagttcgacc agattcttct gcgtagcgca
     4381 aatcacccgc acatcgacat gcacctcatg gtcttcgcca acccgacgga aagtgccatc
     4441 attaagaaaa cgcagtaatt tcgcctgcat ccgtggtgac atttccccta tttcatccaa
     4501 cagcaccgaa ccaccgttcg cctgctcaaa gaaccctttc ttcccttccg gagcatgacc
     4561 aaacagttca ctctcgaccg catcttctgg tatagacgcg caattcagcg ccaggtaagg
     4621 tttgcccgct ctggggcttg cctgatggca ggcgtaggca aaaagatctt tacctgtacc
     4681 tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc agtttctgcg cctgttcgac
     4741 aacgtgcttc attttcgggc tgacggcgac aatttggctg aaggcgctga cgtcctgggc
     4801 ggcgacattt tgcaactggc ggcccatacg aatcgttgat cgcaacatca ccaccgcacc
     4861 ggtcaggacg tgttgatcat tttcatcctg aagataaaca ggcgtaatct ccatcaggaa
     4921 attctgccca ttaataacga catgctcgtt atgcgaatct tgcggttcgc tttccagcca
     4981 acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta tggttgcgca ggcgatccaa
     5041 tttttgccca aaaagctgac agctcgccgg gttcgccata tccactttgc ttttcatatc
     5101 gacagagagc acaggttcag gcaacgcctc cagtaacgcg ctcaacgcca gatgctcacg
     5161 ttcggaaggc atccacggga cagtacgcac atcggtaaca cctgcaatac ggcgtatttc
     5221 ggccatcaaa ctgctgaaac tctcaaactc cagttcagca aaattgaggt agattcgccc
     5281 aatgggatca atctcaatac cgcgtaaatc aatgcctctt agcacgagta gatcgagtaa
     5341 ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc agacgcatgg gaaccttcac
     5401 ctgaaaaaag aacaattaat atgatgatat gacactattt ggatagcagg aaggtttctg
     5461 tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc ggcgtaaaga tatccggtca
     5521 gcacccgata ttgcattatt tttcgctggg cgttttgcct ttttgcagag tttctttcac
     5581 ctgaccgata agctgacgac ggaaatcccc aaggcgaggt ttgtcatcgt caatccacgg
     5641 cagcgggcgg caaagctcca tagctttaat cccgagtcgt gccgtcagaa gtccagcacc
     5701 aattccctga gctgcgcggg tagacaaacg agcagcgaga tcttgcgaca tccagtccat
     5761 ccccacttcg cgcaccagtt cgctggctcc ggcaaaagcg atattcagca ataccagctt
     5821 aaacagacgc aaacggctgt aataccccag ttcaatgcca tacagcgtgg caatgcgatt
     5881 aattaaacgc agattgcgcc aggcgataaa cgccatatcg accaacgcca gcgggctgac
     5941 cgcaatcatc aacgttgatt ccgccgccga acggctgatt tcgcgccgcg cctgggcatc
     6001 taaaactggc tggaccaaat gcgcatacag gctgaccact tcacgatcgt tctgcgtttc
     6061 atggattgag gcataccagc gttgcagcgc cggatgcgac tgatcaatac ccgcctgctg
     6121 cgccagtttt tcgcaaaatg cgcgaccttt gcccgtgccg tggctatgca ataaatcacg
     6181 cgcttcgtcg cgttcatggg cgcgctgtcg caagcgccat aagcgccgcc actctgttac
     6241 cacagaacct acgccagcgc cgataatcaa tgccccagcg gcacatccac ccagcgctac
     6301 ccagtcctgg gtttgccagg cattcattgt ccactgtata ccctgcccga caacgcttgc
     6361 gccaaacaga gccagcccgc ccatcaccat tttgcgccac aggctgcgtt tcggacgtaa
     6421 cgctgcgtcc attaccgctt cgacttgccc ctcttcttcc tgcgcttcgt cgagcgtggc
     6481 cggggcaaaa ttttgcgcct gattttcgtc aaaggtctgc tgcgccctga attttggatt
     6541 ctgttcgacc tccagcggac cgtcgaaatc aatacgtggt tttaacggtt cggtcatcgc
     6601 aatttatctc ctattaaaaa ttccagcgcg gcatcaagac gaatatgcgg cagcggtttg
     6661 tcgacatcca tcacctgcgg gcgaaacgct tcaaactgga acccttgctt gtcccagaac
     6721 gcctgaccgg gcaaacgcgc aggaacttca ccggggtaaa cggtgagagg cgcaccatcg
     6781 ctgagccggt taccgcgcaa tgccggaatt ttctcgccgt tgacgtcaat aatgccgctg
     6841 gtggtcgtct gcaccgaggc cagccccagg caatccatac taatcctttc aaacgccgca
     6901 ttttgccagg catcctgaat cagttgttgc agtaatgaaa ccatattggc gtgctgatcg
     6961 accgttacat ggtccgcttt cgtcgcggca aacagcagtt tgtcgataac cggcgagaat
     7021 aagcggcgaa acagcgtgcg ctgcccgtag tggaaacttt gcatcagctg cgtcagtgcc
     7081 agacgcatat cattaaatgc ctgtggccca ctgttgagag gttgcaggca atccaccagc
     7141 acaatctggc ggtcaaagcg cagaaaatga ttcttataga accccttcac cactttctcg
     7201 cagtaataat taaaccgctc gcgcagcatt ccggcattgg tgtgcttatc ggcctgcgcc
     7261 agtttggact cgccccaggc atcgacatcc ggccacggga agaattgcag cgcgggcgca
     7321 cctgccatat ctcccggcaa gacaaagcgc ccaggctgaa taaagtgcag cccctgctgt
     7381 ttacagtggt ggagataatc ggtccacacg gcggcaatgt ctgccagccg gttttcgtcg
     7441 gcaggcgcta gcgggtcaag tccttcgcac atcattcgcc atttcgccga ccattcgccg
     7501 cgctgaccat tgagtaagcc cgtcatctgg cgcgaccagc ttaagtaatc ctgcgccagc
     7561 atcggcaggt cgagcaacca ttcgccggga taatccacaa tttccagata cagcgtggag
     7621 gtatccttaa agtggcgcag cagcgaatcg ttcgatttaa aacgtaacgc caggcgaatt
     7681 tcactgacgc cgcgcgtagg cgtcggccag gcgggaggat caccatataa ctgcgccagt
     7741 ccttcatcat aggtgaagcg cggaatgcca aagtcacgct ggggaatgcg ttttacgccc
     7801 agcaagcgct cttcacgcac cgcgcttaac agcggcaaac gtgctccggc atgaatattg
     7861 agcaactgat taaccatcgc ggtgataaac gctgttttgc cgctgcggct aagtccggtt
     7921 acagcgaggc gcagatgtct gtcgacaccc cgattcacca gcgcattgag ttcattttta
     7981 agtcgcttca ttgctgtcct gtgctgctct gaagaccaga aaaaaatcat tcgtaaggat
     8041 atgaatgtgg gggccaagcc attgattatc aactgcccgt gttgtcatcg acgggcgaac
     8101 tccataagat gtgcagcatt cacgttcgga cggagttcct gacctatgtc ccctactatt
     8161 tatgatattg ccagggttgc aggcgtatca aaatccaccg tatcacgcgt gctgaataag
     8221 caaactaata tctccccgga agcgcgcgaa aaagtgttac gggccattga agaattacag
     8281 tatcagccaa acaagctggc ccgcgcgctg acctcttcgg gttttgatgc cattatggtg
     8341 atttctaccc gttcgaccaa aactacggcg ggtaatccgt ttttctctga agttttgcat
     8401 gccatcaccg ccaaagcgga agaagaaggt ttcgacgtga tattacaaac gtcgcacaat
     8461 ccggcagaag atctgcaaaa atgcgaaagc aaaattaaac agaaaatgat taaaggcatt
     8521 attatgctca gttcgccagc ggatgagtca ttttttgccc aactcgataa atacgatatt
     8581 ccggtggtgg taattggcaa agttgaaggt caatatgccc atgtttattc tgtcgatacc
     8641 gataattttg gtgacagcat tgcgttgacc gatgcattaa ttgaaagtgg gcatcaaaat
     8701 attgcctgcc tgcatgctcc gcttgatgtc catgtttcgg ttgatcgcgt taatggctat
     8761 aagaaaagtc tgtccgcgca taatattgca gtgcgtgata aatggattgt tgacggcggt
     8821 tatacccatg aaacagcact gcaagcagca cggcaattat taagtcagtc accgttgcct
     8881 gaggcagtgt ttgccactga cagcctgaaa ttaatgagca tttatcgtgc ggcggcagag
     8941 aaaaatattg ctattccgca gcagttagcg gtggtgggtt atagtaatga aacgctgtcg
     9001 tttattttaa cgcctgcacc aggcggcatc gatgttccga cgcaggagtt aggtcaacaa
     9061 agctgcgagt tattattccg cttaatttcg ggaaaaccgt caccacagaa tattaccgtt
     9121 gccacgcata tgacgctgaa ataaatacca gctaccttgc ctgaatgccg gatgcgatgc
     9181 tggcgcatct tatccggcct acatttatag atcggataaa gtactcgcgc cgcatccggc
     9241 aatttagccc attatcagaa cgagtaattt acgccgacac ctgcataatg gaatttatcg
     9301 ctgtcgcctt cgtcgtgatc ctgccactca aacgcgtatt ccagcgaaac ggaaagtccg
     9361 ttctggaaat cataaccgta aaataaaccc acacggttaa aatcatggcc ttcacgttca
     9421 atatcatcct gccagtccca gttactccag cgatccagcc caatgcgcgt atacggcgtc
     9481 accgagtggt tgccgagcgt cagcggcaaa taggcgcgaa tttcttgtgt ggaaaactca
     9541 ccgttattgc ggctgtcgtc catattgaag ccgcgctcga gataatagtt cactcgcaaa
     9601 gcaaccgttt cgttgaaggt atattgcaga cctgtttccg tttcgacacg ggtatcagcg
     9661 taaccggtcg tgttcaaatc gttggcaaat ttatacatcg acaaccaacc gttgaaacgt
     9721 aaatcatcag tcagtttcac atcccagtct ggcgcgattt tccagcgctg catattcgcc
     9781 gtgtcttttc ccggttcatc aacgtagtga taaccataat tacggaaacc gccggtcagg
     9841 ccgaaactga aatcatcgct ttcgaggaac tgataatgca cctccagctc cgggcgatca
     9901 aaccacgttc cacgtttacc cgcgctataa tctaccggcc cttcctgata ataggccaga
     9961 gcaattctcc acggcccgtt ggcggcatta aaatagactg aaggctccgc cagcccatcc
    10021 atatcttcgc cataaccctc gacgttttct atttcgtaca tcgcaccgat attaaagtgc
    10081 cagtcgttct tttcctcggc ctgtgcgcag gccattcccg cacacatcac cagtgcggta
    10141 caaggtaata actttttcat tattgttcct tgtattatcc taggggaatg ccttccccct
    10201 gtatttagcg aattgctatt tccgtttctg catcaaagaa atgacatttc gtcatatcaa
    10261 aatgaatagt gatattttct cctgcatgat aatcatttaa cgcaccagca cggaccacta
    10321 actcgtgccc cccaaccgtg gtgtagagca taaattccgc accggttaat tctgccacgc
    10381 taattttggc ggaaatgtta ttttcctctt gcgcgtccgg atgaatatct tccgggcgta
    10441 ttcccatcac gatgggctta tgcaaacttt cctgtgtttt cagaaccgct aatttctctt
    10501 cgagaatggt taatttaagc gtttccgtaa cgaatttatc gccatcgatc gtgccgcgaa
    10561 taaaattcat cgctggtgat ccaataaatc cggcaacaaa catattcgcg ggttggttat
    10621 aaacggtttt cggcgcacct acttgctgaa caatcccgtc tttcataatc acaatccgcg
    10681 tcgccatggt catcgcttcg gtctgatcgt gggtcacgta gatcatggtg gtgttcagtt
    10741 tctgatgcag cttgctgatc tctgcacgca tttgtacgcg cagcttggca tcaaggttag
    10801 agagcggttc atccattaaa aacacgcccg cttcgcgcac aatcgcccgc ccgagcgcca
    10861 cacgctggcg ttgcccgccg gaaagcgcgc ccggcttgcg tttcaggtac tcacgcaggc
    10921 cgagaatttg cgccgcccag ttcacccgct catcaatcac ctctttggcg attttttgca
    10981 tcttcaggcc aaacgccatg ttgtcgtaaa ccgtcatatg cggatacaac gcgtagttct
    11041 ggaacaccat cgcgatattg cgtgctttgg ctggaacgtc attcattcgt ttgccgtcga
    11101 tcaacagatc gccgccgctg atctcctcaa gcccggcaat catgcgcagg gtggtcgatt
    11161 taccgcagcc cgacgggccg acaaatacga tgaactcttt atcggcaatt tccaggttga
    11221 agtccttcac cacatgcacc tggttatcgt agattttttg aatatgttgt aacgaaagct
    11281 gagccatgtt gattcctttg ctatacgttt tgccagaagg ccgataaccg cgaccaggtc
    11341 agggagtccg ttgagggcaa cagtaattgc gccccggtta agcccgcacc gatccccacc
    11401 gagcgcatac cgctggcgtt aatggcgtca atgcccgcct gcgcatcttc aatgccgata
    11461 catgcctgcg gcggcacgcc cagcccggca caggcagcga gaaagatttc cggatccggt
    11521 ttcgagtttt taagttggga agcatccgcg cagaaggtga aaaactcgcg cagctccagc
    11581 gccgctaaaa tcgtcggcgc attcagggag acggaagcca gccctaccgg aatctgctgt
    11641 gcacggagct ctgccagcaa attgcgaata ccgggcagca cagcattaac cgtcagctcg
    11701 cgcagtgagt ggacgtaaag cagatttttg cgatacgcca gttgcgcccg ctcctgcggg
    11761 ttaaagtcgc cctctttgcc cccgtgttgc agaatgcgcc gcagagactc atcgcggctg
    11821 atccctttta gagattcgtt aaactgcgca tcaatactga tgccaatttc agcggcaatc
    11881 tgctgccacg cctggaaatg cagatgcgcg gtatcggtga ttacaccatc cagatcgaaa
    11941 attacccctt gcagtttcat tcatcctcct gatgcgtggt agcggtccca ttgaagggca
    12001 aaataaaatc acccaaacag aaaacagatt cttcagccac ggatataagc tgaccgttca
    12061 aacgcagtga aacgggcgca gaagttcgaa tcgcaatacg ctgcgcgtcg agagtgacct
    12121 gtaattcgca gccctgccag aacagaggga aagacaactg ttgccactgc tcaggtaacg
    12181 ccggatttag atgcaattca ccgtcacgca cgctcacccc ggcaaaaccc tgaatcgccc
    12241 ccagccagat agcgccagtt gcggcagcat ggataccatc atcacaacta tgcggatcag
    12301 caccaagatc gatttcagtc ccctcgcgcc agaactgata actttgggtc agcaggccac
    12361 agcgtgcagc aacaatgccg tggattgctt tacttaatga cgagtcgtga atagtgcgcg
    12421 gttcataaaa ttgcagattg gcaagacacg atgccgatga gaactgctct ggcagcatgt
    12481 aattgagcat caccacatca gcttgtttga ggatctgcat ctcgttcact tctgcgcgtg
    12541 aataatccag cagaatggtt tgcttccccg ccgccgcttt gtatttcgcc agattaatcg
    12601 ccggcttagt cataaacgaa tcatcctgcg gcaaaacacc gtcgggctga atttctggca
    12661 gccgcagttc tttaaggaac atttcggcac gatgaataaa cgcgtcgtca ctacagccaa
    12721 actggcgggc aatactcagc gcctgctgga cgttgtaata caccatatag ctggtgaagg
    12781 cgttattatt gacatgttcg gtatattcgt ctggcccaat aacatcatga atttccagac
    12841 ggtcgttaac cctcaccgcg cggctaatcc agaactttgc agtttccaga agtagcgcca
    12901 tgccttcatg cgcaatgaaa ctttcatccc ccgtggtctg ccagtattga ataaccgccc
    12961 aggcgatatc ggccaccaga tgatgttccg cctgcgccga ggccactttt tgccgcagcc
    13021 cggtgcgaat gttaatggcg gcaaattccg gcgtctcttc ttcgccgctg cgcgcgcttt
    13081 cccacggaaa tagcgcgccc tgccagccgt tgcgtcgcgc tttctcctgc gcgcctggca
    13141 agttgtgcca gcgataacgc agtaaacttc gggcaaccgt cggatcgcta aacagatgga
    13201 acggcagcaa aaatacttct gtatcccaga aaacgtggcc tttgtagcct tcgccggtta
    13261 agccttttgc cgcaatactg ctgcgctcgc cgtgagccgg cgtcatgatg cgcagatgat
    13321 aaagcgcata atctaacgct tgctgatcgt gcgcatcgcc gccatttacc gtgatacgac
    13381 gtttctgcca ccattgacgc cagttttctg ttgatgctgc aagatgttgg tcgtaactct
    13441 gttgcgcgca cat
//
LOCUS       DADMEQ010000104        13434 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.105, whole genome shotgun sequence.
ACCESSION   DADMEQ010000104 DADMEQ010000000
VERSION     DADMEQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.105"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            140..1492
                     /locus_tag="JNZ05_003061"
     CDS             140..1492
                     /locus_tag="JNZ05_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY8566206.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RYTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKNELVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1505..2263
                     /gene="chbG"
                     /locus_tag="JNZ05_003062"
     CDS             1505..2263
                     /gene="chbG"
                     /locus_tag="JNZ05_003062"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HAY8566207.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2310..4571)
                     /gene="katE"
                     /locus_tag="JNZ05_003063"
     CDS             complement(2310..4571)
                     /gene="katE"
                     /locus_tag="JNZ05_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HAY8566208.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGHVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4754..5017
                     /gene="cedA"
                     /locus_tag="JNZ05_003064"
     CDS             4754..5017
                     /gene="cedA"
                     /locus_tag="JNZ05_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HAY8566209.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5294..6109
                     /locus_tag="JNZ05_003065"
     CDS             5294..6109
                     /locus_tag="JNZ05_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566210.1"
                     /translation="MRREMFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFIS
                     TGQCDCDEIAESNFNRMIAYMSNINYDASKLGAPALSFDTYLQDNVKYRVIINNLYGS
                     EFRIRDINEDFVGMDFTCEFRPEKMTNLINIKNRLVIHYLRTIYYEKNYIHPAGSIFA
                     AIQKNESLLKFPSIISMLNINLLFNPLNLPGMGSGVLEDIMSIPDSSLRKRLGYEVLS
                     FSLQAHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(6113..7503)
                     /locus_tag="JNZ05_003066"
                     /pseudo
     CDS             complement(6113..7503)
                     /locus_tag="JNZ05_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
     gene            complement(7636..8226)
                     /locus_tag="JNZ05_003067"
     CDS             complement(7636..8226)
                     /locus_tag="JNZ05_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY8566211.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(8389..9056)
                     /gene="hxpB"
                     /locus_tag="JNZ05_003068"
                     /pseudo
     CDS             complement(8389..9056)
                     /gene="hxpB"
                     /locus_tag="JNZ05_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
     gene            9203..9739
                     /locus_tag="JNZ05_003069"
     CDS             9203..9739
                     /locus_tag="JNZ05_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566212.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(9780..10640)
                     /locus_tag="JNZ05_003070"
     CDS             complement(9780..10640)
                     /locus_tag="JNZ05_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAY8566213.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(10746..11036)
                     /gene="ghoS"
                     /locus_tag="JNZ05_003071"
     CDS             complement(10746..11036)
                     /gene="ghoS"
                     /locus_tag="JNZ05_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAY8566214.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(11137..12066)
                     /gene="pfkB"
                     /locus_tag="JNZ05_003072"
     CDS             complement(11137..12066)
                     /gene="pfkB"
                     /locus_tag="JNZ05_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HAY8566215.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVSRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            12352..13106
                     /locus_tag="JNZ05_003073"
                     /pseudo
     CDS             12352..13106
                     /locus_tag="JNZ05_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
     gene            complement(13159..13266)
                     /locus_tag="JNZ05_003074"
     CDS             complement(13159..13266)
                     /locus_tag="JNZ05_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566216.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
BASE COUNT         3448 a         3116 c         3342 g         3528 t
ORIGIN      
        1 gatatcgtaa gaaattgacg gaatttaatc agtaagtcgt tatacctgac aattcacata
       61 tctgtcctgt tgctggatta tttatgtccg ggggggcaga tataccagat atcagtattc
      121 tgtactgaag ggagaaatta tgagccagaa attaaaagtc gtcactattg gtggcgggag
      181 cagctatacc ccggagttac tggaaggatt tattaagcgt tatcacgaat tgccggtcag
      241 cgaattatgg ctggtggatg tcgagggtgg caaagctaaa ctggatatta ttttcgatct
      301 ctgccaacgg atgattgata acgctggcgt cccgatgaag ctttataaaa cgctggattg
      361 ccgcgaagca ttgaaagatg ctgatttcgt tactacccaa ctgcgcgttg gccaattacc
      421 ggcgcgtgaa ctggatgaac gtattccatt aagtcatggt tatcttggtc aggaaaccaa
      481 cggcgcgggc ggtttgttta aaggtctgcg taccattccg gtgatttttg acatcgtaaa
      541 agatgtcgaa gaactttgtc cgaatgcatg ggtgattaac ttcactaacc cggcgggaat
      601 ggttactgaa gccgtttatc gttataccgg atttaaacgc tttatcggcg tgtgtaatat
      661 tccgatcggc atgaagatgt ttattcacga tgttctgatg ctgaaagaca gcgatgattt
      721 atctatcgat ttgtttggcc tcaaccatat ggtgttcatt aagaatgagc tggtaaatgg
      781 caagtcgcgc tttgccgaat tgcttgatgg tgtggcatcc gggcagttaa aagcatctgg
      841 cgttaaaaat attttcgatc tgccatttag cgaaggctta attcgttctc tgaatctgtt
      901 gccgtgttct tatttgcttt attacttcaa gcaaaaagag atgctggcta ttgaaatggg
      961 cgaatactac aaaggcggcg cacgagcgca ggtagtacag aaagtcgaga aacaactttt
     1021 tgagctgtat aaaaacccgg agttgaaagt taagccgaaa gaactggaac agcgcggtgg
     1081 ggcttattac tctgatgcag cgtgcgaagt gatcaacgct atctacaacg acaagcaagc
     1141 tgaacattac gttaatatcc cgcatcatgg gcatattgat aatattccgg cagactgggc
     1201 ggtagaaatg acctgtaagc tggggcgcga tggcgcgacg ccacatccgc gcattacgca
     1261 tttcgatgat aaagtgatgg ggctgattca caccattaaa ggcttcgaga ttgctgccag
     1321 caacgccgca cttagcggag aatttaacga tgtgttactg gcgctaaacc ttagtccgtt
     1381 ggtgcattcc gatcgcgatg ctgagctgct ggcacgcgag atgattctgg cgcacgagaa
     1441 atggctgcca aattttgccg actgcatcgc agagcttaaa aaagcacatt aaccgaggct
     1501 gattatggaa cgcttactga ttgttaatgc cgatgatttt ggcttaagca aaggtcagaa
     1561 ctacggcatt atcgaggcct gtcgcaatgg gattgtcaca tcgacgacgg cgctggtgaa
     1621 tgggcaggct attgaccatg cggtgcagtt gagtcgtgat gaaccgagtc tggccatagg
     1681 gatgcacttt gtccttacta tgggcaagcc actgacagct atgccggggt taacccgcga
     1741 tggtgtgctg ggaaaatgga tctggcagtt ggcagaagaa gatgctttac cgctggaaga
     1801 aattactcag gagcttgcca gtcagtattt gcgtttcatt gagctatttg gacgcaaacc
     1861 tacgcatctt gatagccatc atcatgtgca tatgttcccg cagattttcc cgattgttgc
     1921 caggtttgcg gctgaagagg ggattgcgtt gcgcatcgac cgtcagccgc tatctaacgc
     1981 tggtgattta ccggctaatc tgcgcagttc gcagggattc agtagcgcgt tttatggtga
     2041 agagattagc gaggcactgt tcctgcaagt gctggatgac gcaagccatc ggggtgatcc
     2101 ttcgctggag gtgatgtgtc atccggcgtt tatcgataat actatccgtc agagtgctta
     2161 ctgtttccca cggttaacgg agctggaagt gctgacatca gcgtcgttga aatatgcaat
     2221 tgctgagcgt ggttatcggt tgggtagtta tcttgatgtg taagtaaggt ggtaaggtca
     2281 ctccccaggg gacgtgacct tatccatatt tacgccggaa ttttgtcaat cttaggaatg
     2341 cgtgaccaca cgcggtgttc tgccatcagc gttaacagtt catccataaa actaccgtcg
     2401 gtgctgtcag cttccacaat cccttcttca ccctggtcag ctaccttgat tgttgcttta
     2461 aacttgcgcg cgtctcccgc cagcgcaatc ggtttaaggt gtttgtaggc ttccatcaga
     2521 tagtagttgg catcgccgtt gtcagcgata tccgcgatat tgccgcaagg gacaatgacc
     2581 gcatcgaccg tcagcgaagg tgcaccggca aaggtagcgg ctataggcaa caccgtaccg
     2641 tcatccgcag tcacttcacc cattcgggag tagagcagtt tggcatgaac gcctttggcc
     2701 ttcagggcct tgagaatggc aagaaggtct gccgatctca cttcatcatt aagtaaaatc
     2761 gctaccacgt gacctttcac atcaccgtca ggaatggcgt acaaacttaa ggatggatcc
     2821 tttttcagac cgttgacgtc cggaggtggg gtgatattca gctggtcgtc agtcagttcg
     2881 ataccgagat ttttcgccac cgcctgggcc agagtgagat caatatgcgc cagctggtca
     2941 acaacgcgct cacgaatata cggacgaacg actttgctta actcaaaact gaaaccatcg
     3001 acaatatggc gctgctcgaa tggcgtctga cttagccaga acagacgcgg atgggaataa
     3061 tattcgccaa acgatgggct gcgctcgcga actttattgc cttccacgcg ctcctggtat
     3121 gattcaaaac cgccgcgttt cggccccggt ggtgtttcgc gcggccagtt atcgttgatc
     3181 gagttcggtt cataattcgc cgggttagtg tcgatcccca tacgatgcat gccgtcacgc
     3241 tggaaattat ggtaagggca ggtcgggcgg ttaatcggaa tctcatggaa attcggccca
     3301 ccaagacgac tgatttgtgt atcggtatag gagaacaaac gtccctgcaa caggggatcg
     3361 ttggtgaagt ccagaccagg aacaatatga cctggatgga atgctgcttg ttcgttttcg
     3421 gcaaagaagt tatccggatt gcgattgagc accattttgc cgacacgctg aacgggcacc
     3481 agttcttccg ggatcagttt ggttggatcg agaagatcga agtcgaactt gaattcatct
     3541 tcttcaggaa tcaactggaa gcccagttcg tattccggaa aatcgcctgc ttcaatggct
     3601 tcccacaact cgcggcggtg gaagtccggg tcacgtccgg tgagtttttg tgcttcatcc
     3661 caaacgagtg aggctttacc tgccagtggt ttccagtgga aacgtacaaa cgttgccttc
     3721 ccttcggcat taatcaggcg gaaggtgtga ataccgaagc cttccatggt gcggtaactg
     3781 cgggggatgc cgcgatccga catcgcccac atcacgttgt gcagagtttc aggttgcaga
     3841 gaaacataat cccagaaagt atcgtgggca ctttgccctt gtggaattgc ccagtgcggt
     3901 tctggtttta ccgcatgaac aaaatcgggg aatttatgcg catcctggat aaagaagatt
     3961 ggcgtgttat tgccaacgag gtcaaaaata ccctcttcag tatagaactt ggtggcaaaa
     4021 ccacggatat cacgcacggt atcggcagag ccagcgccac cctgaacggt agagaaccta
     4081 acaaatactg gggtgatttt gttcggatct gagaggaaat ccgctttggt gatatcgctt
     4141 aagcttttat atggctggaa ataaccgtga gctgctgatc cgcgtgcatg aacaatacgt
     4201 tccgggatgc gctcatggtc aaagtgggtg attttctcgc gcagaataaa atcttccagc
     4261 agcgttggac cacggttacc ggcacgcagt gagttttgat catcggcgat gcgcacgccc
     4321 tgattagtgg tcagcgcata attttcactg cctttgcgta cgtcttccag agaattaagt
     4381 ttttcgttac gtgtatcagg ggctttcagg ctcccagggg cggtaggttg tgcacctggc
     4441 ggtgttggtt cagccgctgg acgatgagat ccgtcctcag gtgccagtga gtccatcccc
     4501 ggtttcgctt cgctggaatc gtgtagtggt gactggtgct gatgtgggtt cttttcgtta
     4561 tgttgcgaca ttgaactcgt ctccttaatt tattactgga agggccgctt ttttggtcaa
     4621 aaaactctct aactatagac caccagccag atcccgcttc ggagactatt ctggaaacgg
     4681 tgttatgaaa tacacgcaaa gcgggacgta caggggctaa atcggctaaa ctacagtttt
     4741 tctaattttt aatatgcgtt tagtgaagcc agtaatgaag aaaccgctcc gccagcaaaa
     4801 ccgccagatt attagctatg tcccacgcac ggaacccgcg ccgccagaac atgcgataaa
     4861 gatggattcg tttcgtgatg tctggatgct gcgtggcaaa tatgttgcgt ttgtactgat
     4921 gggagagtca tttctgcgct caccggcgtt tactgtgcct gaatctgccc aacgttgggc
     4981 aaatcagatc cgccaggaag gggaagtgac tgagtaagga ttacatacct cctggttttg
     5041 ccgggagtta caggacctga aattctgcat ttcgttgcta cattcattca aaccacctgt
     5101 aacaaaaagc tgcctttgac gagctattgc tataaataag tgatcttttt tctttaattg
     5161 tttatttatt ttaaaatggt taagttttgc taatcatgat gagttatttt tgatagttca
     5221 tcctgtggcg tcgctcttaa acttatattg taaatggctt agatttaaat aagataataa
     5281 ttttgttaac aatatgaggc gggagatgtt ttctacaaac ttaatacaga gtaattatgg
     5341 tgatttaaat attaaaagcc tggctttcga ctcttttaaa gaaaggcttg agtctaccat
     5401 ggcggcactc actttcttta tatcaacggg tcaatgtgac tgcgatgaaa tagcagagtc
     5461 gaacttcaat cgtatgattg cctatatgtc taacattaac tatgatgcct caaagctagg
     5521 agcgccagca cttagttttg atacgtatct gcaggacaat gttaaatata gagtgataat
     5581 taataatctt tatggcagtg agtttcggat aagagatatt aatgaggatt ttgttgggat
     5641 ggattttacc tgtgaattcc gacccgaaaa aatgacgaat ttgatcaata taaaaaatag
     5701 gttggttatt cattatttaa ggactatata ctatgaaaaa aattatatac atcctgccgg
     5761 gagcattttt gctgcgattc aaaaaaatga gagtctccta aagtttcctt cgataatatc
     5821 aatgcttaat atcaatcttc tttttaatcc actaaatctt ccgggaatgg gaagtggtgt
     5881 gcttgaagat attatgagta ttccagatag ttcacttaga aaacgtctcg ggtatgaggt
     5941 tttaagcttc tcactacagg cacattcatt gagccaggaa tgtattgata agttggacat
     6001 ttttttcgct gacgatctgt ttaaatatga gtcagtatgc atcgccgcga tggaacattt
     6061 aaaaagtaaa gcaacggccc ctatacaaaa cggaccgttg ccagcataag aattaacggt
     6121 gtgccagttc ggcgtcgtct tcgctatcca gaatggcttt atcggtttgc ttcagccact
     6181 ggctggtcag cgtgccagct gtcatcgagc cactaacgtt tagcgccgta cggcccatgt
     6241 cgataagcgg ttcaacggag attaacagcg ccaccagggt tactggcagg cccatcgcag
     6301 gcagcacaat cagtgcggcg aaagttgcac caccaccgac accggcaacg cctgcggaac
     6361 taacggtaac aataccgacc agcgtcgcaa tccacatcgg gtccagcggg ttaatgccaa
     6421 ccgtaggcgc aaccatcacc gccagcattg ccggatacaa accggcgcag ccgttctgac
     6481 caatggttgc accgaaagag gcggcgaaac tggcgatgga ttcaggaacg cccagacgac
     6541 gcgtttgtgc ttccacattc agtgggatag acgcagcact ggaacggctg gtaaaggcaa
     6601 acgtcagcac aggccatacc ttacggaagt acttcagcgg actcacgcca ttaatgccca
     6661 gcagaatgcc atgcacagca aacataatca gcagaccgag gtaggacgcg acaacgaaac
     6721 ttcccagttt gatgatgtct tgcaagttag aacctgcaac cactttggtc attagagcca
     6781 gaacgccgta aggggtcaac tgcatgacaa ggcgaaccag tttcatcact cagctttgta
     6841 gggtatcgat agcggttaag acgcgttcac ctttcggcgc atcatccttc agcagtttca
     6901 atgcagctac gccgaggaat gcggcaaaaa ttaccacgct gataattgac gtcggattgg
     6961 ctccggtaag atcggcaaac gggtttttcg ggataaagga caagaccagc tgcggaacgc
     7021 tcagatcaga gactttacca acatagttac tttcaatggc gttcagacgt gcagtttctg
     7081 caccaccctg aaccagacct tcagccgtca aaccaaacag gtggtgacca gcacaccgac
     7141 cagcgccgca atcagcgtgg taaacaaaag cgtaccgatg gtcagaaaac tgattttgcc
     7201 taactgagat gcgttatgca gacgggcaac cgcgctcaga atagaggcga agactaacgg
     7261 cataacgatc atttgcagca gttgaacatt gccgttacca acgatgttaa accactgtac
     7321 agaatcttta agtacctggc tgtcagaacc ataaatggta tgcagggcaa ggccaaaaac
     7381 cacacccatc accagaccca ccagcacttt tttcgccaga ctccactgtt tatggcgggt
     7441 ctgagccagc gcaaacagca gtacaacgaa caccacgatg ttcgcaatta atggaaagtt
     7501 catccccgtt ctcctgatct tattatttct atcactgcgt agcagtcata gtgttggcgc
     7561 aagattaaca gaactgtgag ctggctctta tattcaaatg gaattgctta ttccagaaag
     7621 gtgatttttg cctttttagt attcaacctg atgcttaata agccgatgaa actggatctg
     7681 caaggtattg atcatttgcg atgaccaacg aacagcgctg ttctcgggta atgaatgggg
     7741 catccatacc gaccagacaa ataatgccat gcagataaaa cgttccagtt ggttgccctt
     7801 ttggggaacc aggataggca agcggaaacg ccagcggcat ggccagagca ggggaacacc
     7861 ggcgggtgtc agcatatcgg caagtatgtg gctcaaataa cccagcacca ttccttgtag
     7921 cgcatcagcc ggaatgaacc agccttccgg aaccttaagg taaaaggttg ccagcagcgc
     7981 aaataccgcc agcagactgt gggtaaaacc acgatgccca aaagcgcggg cgatcggttt
     8041 tgatatccat tttaatcgct gcccaagaaa cgactttggg tgatcgatgt ccggtaacaa
     8101 acacgtcagg attgcggaag ggacaatatg ccaccagtca ccctgtgcca gcacgggcgt
     8161 cagctcggca tttttggcaa ataccgcaca agcaatagaa aagagaaggt gaccttccgc
     8221 cgtcatgata aaactcacta aactgtcgat acgtacagta taggattttt atacagtgca
     8281 cggaagtggt gacggtgtaa ctctttctct taaattgacg attaatccgt cagttgaatg
     8341 caaattcaga taaagaacca ggggcagcgt gatcactgcc cctggtcatt aaccgagaag
     8401 gtcttttgcg gtgagttctg tcagcgatga gagtttgacg tttgctaata caaaacgtgg
     8461 atcattttgc gcttctggcg caggaacgac gatggaacgc atgcaggctg ctttagaagc
     8521 gatcatgcca tttaccgagt cttccagcgc cacgcaggtc agtgggtcaa cgcccagttt
     8581 tgctgcgcag tcgagatata cttgcggatg cggcttgctg taaggcagtt tttcaaccga
     8641 ggcgagggca tcgaaactgt cgcgtaagtc aaacatggtc aacacttttt ccagcatatg
     8701 tagtggtgac gcggaggcca gtcccaccaa taaaccttgt tctttgcata acgcaacagc
     8761 ttcgcgcacg cctggtaata atggacgtgt ctcttcaacc agtgaaatgg cacgggcaat
     8821 aacccgttct actacttcct gacggcttgg cccattccac ggttgccggg cgtacaaaga
     8881 tcgaccacca tatcgatgcg taaacctaag gtgtccggca gctcgttacg acgggagata
     8941 tctaccccca ggcttgccat cacatccagt tcggctcgat cccataaagg ttctgagtcg
     9001 ataagtaatc catccatatc aaaaattgca gcaagaatct gacgcggggt tgacattaca
     9061 acctctcctt tagagcatta tgtgtaaggt attaagccgt gcttatgatt aagcgcctat
     9121 catatcgcct ttaaagaatg ggcgaaaaca gatatctaca ggtaaactaa gtgaaacgac
     9181 gcgtcttaat caaggagaac tcatgacgta tcaacaagct ggacgcattg ctgttttgaa
     9241 aaggattttg ggttgggtga tttttatacc tgcattgatc tctacattga tttctttgct
     9301 gaaatttatg aatacccggc aggaaaacca ggaaggcatt aatgcggtca tgctcgactt
     9361 tgctcatgtc atgatcgata tgatgcaggc gaatacgcct tttttgaatc tgttctggta
     9421 taactccccg acccctaatt ttaatggtgg tgtgaacgtt atgttctggg tgattttcat
     9481 cctgattttt gtcggactgg cgttgcagga ttccggtgcc cggatgagtc gccaggcgcg
     9541 cttcttgcgg gaaggtgttg aagatcaact cattctggaa aaagccaaag gggaagaggg
     9601 gctaacgcgt gaacaaattg agtcccgtat tgttgttccg caccatacta ttttcctgca
     9661 gtttttctca ctatatatcc tgccggttat ttgtattgct gcaggttatg tgttcttttc
     9721 tctgcttggg tttatttaat caaaaggccg cacggagcgg ccattatcct caacccatat
     9781 catgctgcta ataatctatc caatgactgc tgagcaataa ccaaatgctg accgccaaat
     9841 aatcttgcac gatttaacag cgtgtagagt tggtaaaccg gttgacgttc aaggaaatcg
     9901 gcaggtagcg gggatactga ctgatagccg tcatagattt gtggcggctg ttcagtatgc
     9961 agcggtaaca tcgccaggtc gcactctcgg tcaccccagt agcaggcagg gtcgaaaatg
    10021 tacgggccat ccggacccag cgcacagttg ccggaccata aatcgccgtg caacagagaa
    10081 ggctgcggtt gatgcgaggc cagacgctgc tgaatatgct cgacgatggc gtcgatattg
    10141 ccgaaagcga tccctttctc tgctgccagt tccaactgcc agccaatccg ttgttcagca
    10201 aaaaacgttg accagcgacg ttgccaggtg ttgggctgtg gagttgtgga gagcgagtta
    10261 tcaaaatcga ggccaaattg tggttggtca ctccattgat gtaaacgcgc aatttgctga
    10321 ccaagaataa atgcgctatg cgcatccagc ggacgaggtg ggagataatc catcaccaga
    10381 aaactgtagt cacggtcagc gcctactgcc caaaccttag gcacggtgac ggttttacta
    10441 cgcgacagta actccagttg gtcggcttcg gcggtaaaac cgggaagcag ttccctttca
    10501 tcacatttga cgaaaaagtc atgtcctgca tagcgcaaat gccatgcggc atggacttct
    10561 ccgccaggca gttcattacg cagttcgatt tcgccttcac ctaactgctc gctcaaaaga
    10621 cgactgattg cctgccacat gatgtctctc ccatgttgtc tgccagatca taaggttagc
    10681 gcaagaatgc ggtgtaaaaa cacgagctaa cgcacaccac agactgatta ttgcccaaaa
    10741 accatttatt ttctgttgct attccattcc tcccaggtcg tgatggtgat ttcaggatct
    10801 ttgccggttg cactttgggt aagccccgaa accagttctc taatttcttc ttcactttgg
    10861 gtactgataa gtccaaaagt gttagtcccc agctcatgga tatttccctc atcatccgtc
    10921 atggtgagca aaaaaccgtc gcgagtcagg taattattca actcgttaat ttcagtcaat
    10981 gtatcctcat gcaacatgac ggttatgaca taacggacaa gatcgccact ggccatagtt
    11041 cacccctttg ttatcacatg acttttgagc atagtcggag aaacgcgttg ccgacaggtt
    11101 ggtggtgatt cccccaatgc tgggggaatg tttttgttag cgggaaaggt aagcgtaaat
    11161 tttttgcgta tcgtcatggg agcacagacg tgttccctga ttgagtgtgg ctgcactccc
    11221 cgcagctacg ccaaaacgga ccatctcttc aagagaggca ttttctgcca gtttcagtgt
    11281 catcgcgccg accatgctgt caccagcgcc aacggtactc tggcttttca ccggtggtgg
    11341 caccacctga atgcagtttt cactatcaac acccagcgct ccttgtggac caagggaaac
    11401 gacaacccgt ttggccttgc cgctattaac gatttcctgc gcggctttgc ggacatcgtc
    11461 tggctgggtg agttcgcgac tcaccagcgc actgagttct ttttggttag gcttaaccaa
    11521 ctcgatgtta ccaatcgcca gtgctgcact taacgcttcg ccagaactgt cgatgatgca
    11581 gcggatccct tggttttgcg cagcggaaat cagttgggtt aatttttcca gcttcacacc
    11641 tggcggcagg cttccgctta tgaccaggat ggccccggat tcaatttcca gaacttgctc
    11701 ttcaagctgg cgaaactcat cttcatttaa tgccgcgcct ggcataacaa aacgatactg
    11761 ttcaccgctt gcttccacat gtacgtgtaa attctgccgg gtccagtctt tggcttctac
    11821 agtagcgacg gggacatttt catccgccaa cagtgaaacc aggtgttcgc cggtcgcgcc
    11881 acccgccggg aagatcgctg tggcactgcc tccaagatgg gcaatggcgc gggcgacgtt
    11941 gatgccgccg cccccgggtt cgaacaccgg tgcggtacag cgcagttttc cttcgggata
    12001 aatttgcggg gtaattgttg cgctatcgag agagggcgca agtgtcaacg tatagatacg
    12061 taccatcatt tcctcctata ggctgatttc agtctggcac cgaatcagcg gaaagtgaag
    12121 aaattaacaa tatgatttat tagagcttta tttaaaattt tgcagataaa tatatataaa
    12181 taaaaatctg ttttcgccac atgcttattc aaaaaatgaa ataagaatac attgctaagt
    12241 tattcgcgcc tttttataat tcgttacctt tccagggacg ctaatctttg atccttaaga
    12301 aagtttccgg gaccgtgatg gtctcttttt tgttgtacgg actcttgata aatgaagctt
    12361 ttgaagacag ttcctgccat cgttatgttg gcggggggca tgtttgcgtc actgaatgca
    12421 gctgccgatg attccgtttt tactgtcatg gacgatcctg ccagcgctaa aaaacctttc
    12481 gaaggtaatc tgaacgcagg atatcttgca caatcaggca atacgaaaag ctcctcactt
    12541 acggctgaca ccaccatgac ctggtatgga caaaccactg cctggtcgct gtgggggaac
    12601 gccagtaata cttcttctaa tgatgagcgt tcttcagaaa aatatgcggc gggcgtagcc
    12661 gctttaattt aactgattat gactatttat ttggtcaggc aagctggctg acagaccgtt
    12721 ataacggcta tcgagagcgc gatgtgttga ccgcgggtta tggtcgtcag tttttgaatg
    12781 gcccggtaca cagtttccgc tttgaattcg gtccaggtgt gcgttacgac aaatataccg
    12841 acaatgccag tgaaacccag ccgctgggct atgcctccgg ggcttatgca tggcagttga
    12901 ctgacaacgc taaatttacg cagggtgttt cggtctttgg cgcggaagac acaacgctga
    12961 actccgaaag cgcattaaat gttgctatta atgaacattt tggactgaag gtggcttaca
    13021 acgtcacatg gaactctgaa cctccagagt ctgcaccgga acataccgac cgtcggacaa
    13081 ccttatcgct gggctacagc atgtaaaatt atctgccgga ccgtgttatg cggtctggct
    13141 gtcttcgttt gtcactgctc agccccaaat cattgctgcc caacgtagca acaacatagc
    13201 gccacagatt gccagtagca ccaccaccat cttccagtgt tttttatcaa agcgtttagt
    13261 cggcatacca gtgtcctgca tatctcttgt gcgtgataac ggagtgtaac aaaagggaca
    13321 ggcatcaagg gattttttgt tattacagcc gtcgggtggc gttgtgcaca tggtcactgt
    13381 ttctgcgttt tatgaaaaaa ataaaattat gtatcgatat atatcgtaaa tagc
//
LOCUS       DADMEQ010000105        13405 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.106, whole genome shotgun sequence.
ACCESSION   DADMEQ010000105 DADMEQ010000000
VERSION     DADMEQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.106"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..208)
                     /locus_tag="JNZ05_003075"
     CDS             complement(<1..208)
                     /locus_tag="JNZ05_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8566217.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQ"
     gene            complement(375..1115)
                     /locus_tag="JNZ05_003076"
     CDS             complement(375..1115)
                     /locus_tag="JNZ05_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HAY8566218.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1208..1843)
                     /gene="argO"
                     /locus_tag="JNZ05_003077"
     CDS             complement(1208..1843)
                     /gene="argO"
                     /locus_tag="JNZ05_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HAY8566219.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQHIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(1982..2842)
                     /locus_tag="JNZ05_003078"
     CDS             complement(1982..2842)
                     /locus_tag="JNZ05_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HAY8566220.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3033..4112)
                     /gene="fbaA"
                     /locus_tag="JNZ05_003079"
     CDS             complement(3033..4112)
                     /gene="fbaA"
                     /locus_tag="JNZ05_003079"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAY8566221.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4327..5490)
                     /gene="pgk"
                     /locus_tag="JNZ05_003080"
     CDS             complement(4327..5490)
                     /gene="pgk"
                     /locus_tag="JNZ05_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAY8566222.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5540..6559)
                     /gene="epd"
                     /locus_tag="JNZ05_003081"
     CDS             complement(5540..6559)
                     /gene="epd"
                     /locus_tag="JNZ05_003081"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HAY8566223.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            6931..7362
                     /locus_tag="JNZ05_003082"
     CDS             6931..7362
                     /locus_tag="JNZ05_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY8566224.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7385..7963
                     /locus_tag="JNZ05_003083"
     CDS             7385..7963
                     /locus_tag="JNZ05_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAY8566225.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            7964..8272
                     /locus_tag="JNZ05_003084"
     CDS             7964..8272
                     /locus_tag="JNZ05_003084"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566226.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            8288..8671
                     /locus_tag="JNZ05_003085"
     CDS             8288..8671
                     /locus_tag="JNZ05_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAY8566227.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            8659..9336
                     /locus_tag="JNZ05_003086"
     CDS             8659..9336
                     /locus_tag="JNZ05_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAY8566228.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9330..10007
                     /locus_tag="JNZ05_003087"
     CDS             9330..10007
                     /locus_tag="JNZ05_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY8566229.1"
                     /translation="MLTLNKISYRWPGAATDCLCGISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(9979..10692)
                     /locus_tag="JNZ05_003088"
     CDS             complement(9979..10692)
                     /locus_tag="JNZ05_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HAY8566230.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10689..11198)
                     /gene="fumE"
                     /locus_tag="JNZ05_003089"
     CDS             complement(10689..11198)
                     /gene="fumE"
                     /locus_tag="JNZ05_003089"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HAY8566231.1"
                     /translation="MSTLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(11220..12185)
                     /gene="yggF"
                     /locus_tag="JNZ05_003090"
     CDS             complement(11220..12185)
                     /gene="yggF"
                     /locus_tag="JNZ05_003090"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY8566232.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVICEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            complement(12182..>13405)
                     /locus_tag="JNZ05_003091"
     CDS             complement(12182..>13405)
                     /locus_tag="JNZ05_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708695.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY8566233.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGIIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
BASE COUNT         3196 a         3646 c         3277 g         3286 t
ORIGIN      
        1 tttgatacag ttcctgaccg gctttattga ggctgatatt attatctttg cgggtaaata
       61 gcgtatagcc aatttgttct tccagcgcgg caatgctttt accaaatggc gaagccgtca
      121 tatggatttt ttctgccgcc cgggcaatat tattggtttg cgctaataaa atgaaattgc
      181 gcatcttttt cgaaataaaa atgtccatcc cccctccccg catccttgtc cgcttaaccg
      241 tttcagtcag cctatccttg atgaaaccgc gagcaaagat aggtgattac gtcatggttt
      301 tacagaaaat tacagaaaaa ggaggcaata tcgggtaaag gcattagccc gacgaatacg
      361 tcgggctaca agtattattg tgctgcaggt gttttagcgg gttgttgatc cacaggttct
      421 aactggaaga ccacatcgac ctgatcatca aactgaatag cggcctgctc gtaagtttcc
      481 tgggcagaca ccggcgcggc atcggctttc atcatccgca ccattgggct gggctgatag
      541 ttggaaacat ggtagcgcac gctatatacc ggccccagtt tacgatgaaa gccgttcgcc
      601 agttcctgcg cctgatgaat cgcgttatca atcgctgcct tacgcgcttt gtctttatag
      661 gcatccggct gcgccacgcc cagcgacaca gaacgaattt cgttaagacc cgccttcagc
      721 gcgccatcca gcaaggaatt cagtttgtct aactgacgga gcgtgacttc caccgttctc
      781 acagctcggt agcctttcag gatactttta ccatcctgat agtcataatc tggctgggtg
      841 cgtaaattcg ctgagctgat atcttttttc gcgatctgat tgagttcaag gaaggaaatg
      901 tattgtgcga cgcgctcatc tgcctgtttc ttggctgtag cggcatcctt cgcggccacg
      961 ttaacttcaa tcgcaagagt ggcaatgtct ggcaccgcat ccacacttgc cgtaccggag
     1021 gtgacaatat gcggtccatc cggcaattcg ttagcctgcg ctgccatccc gctaataccc
     1081 attaatgccg ccagggcgat aactttgaac ttcacgttag tctctccgtg ttaattgact
     1141 tgcccaaaat tcaggacctc tggaggcaag cttagcgcct ctgttttatt tttccatcag
     1201 atagcgccta actgaacaag gcttgtgcat gagcaatacc gtctctcgcc agctgcaagg
     1261 ctataaacca catgacacat cccactacca gattgataat gtgctgtgat tttgccgtgc
     1321 gcaggcgcgg tgccagccag gctgcgagaa tagccagacc aaagaaccac aggaaagagg
     1381 cgctaattgt cccgagcgca aaccaacgtt ttggttcgac gtcaagctgc ccgccaaggc
     1441 tgcccagtac aacaaaagta tccaggtaaa catgcggatt cagccaggtc actgccaaca
     1501 tggtggcgat aattttccat ctgccttgct tcaggacttc ggcactagct aactcaatat
     1561 tactactcat cgctgtttta aaagcgccaa aaccatacca gagcaagaag actacgccgc
     1621 cccaagtgac cagcgctagc aaccacggcg actgcatcaa taacgcgcta ccaccaaaaa
     1681 tccccgcgca aatcaggacc aaatcgctga tagcgcaaag taaggcaatc ataatgtggt
     1741 actgacgacg aatgccctga ttcatcacaa aagcattttg tgggccgagc ggcagaatca
     1801 tagctgcccc aagagcaaga ccttgaaagt aataagaaaa cacgttggat attccgaatt
     1861 aagtatctga tgcagagact atatcgcggg attgttatta gcggaaattg ataatattaa
     1921 ttggcaatca gaataactaa taagagagag gccagagagt attatctggc ctcagcgttg
     1981 attacgcagc tttgtcttct ttcacccgct taaagttcac atccatttgc gggtacggga
     2041 agctgatacc ggcggcatca aattcacgtt taatacgctc cagcacatcc cagtacacgt
     2101 tttgcagatc gccgctgttg ctccagacgc ggaccacgaa attaatcgac gatgcaccaa
     2161 gttcgttcag gcgcacagtc atttcgcgat ctttcaaaat gcgatcttca gactggataa
     2221 tattggtcag gatctgctta acctgatcga tatcggaatc atacgccacg ccaataataa
     2281 attcgttacg gcgaactggc tcgcgggaga agttaataat atttccggca ataattttac
     2341 cgttcggaat aacgataatt ttaccgtctg cagtacgcat ggtggtggag aaaatctgca
     2401 cactcagcac agtaccggct acgccgccga ggtcaacata ttctccggca cggaacgggc
     2461 ggaacatgac aagtaacacg ccagcggcca ggttagaaag tgacccctgc aaagccagac
     2521 caacagctaa gcctgcggca ccgagtacag caatgactga cgctgtttgt acacccacgc
     2581 gtcccagggc agcgattagc gtaaaggcga taataccgta acggactaat gcagaaagaa
     2641 aatcagcaac agtggcatcg attttacggg agatcatcag gcgattcacc gcgttggaaa
     2701 tcatccgcgc gataatcaaa ccaacgatga tgatcgcgag tgccgccacg atgtttactg
     2761 cataacttag cagcagcgcc tggttagcta ccagccagct tcccgcgccg tttatgctat
     2821 cgacaacatt caaatcttcc attcaatatt ccttttcaac tgactcaaaa tggagaaata
     2881 cactccgccg ccttatgacg ggcagtctga caagggtaaa caataaagca taattttgcc
     2941 aaatagatca cagatttctc atctgaatca ccttgctttt ggcgaaaaaa agacccgcag
     3001 agcgggcctt gagataagca gaaaggaatg acttacagaa cgtcgatcgc gttcagttcc
     3061 tggaatgctt tttccagacg agcgatcatc gaagtctgac cggcacgcag ccatacgcgc
     3121 ggatcgtagt atttcttgtt cggctgatct tcgcctttcg ggttacccag ctgaccctgc
     3181 agataagctt cgttcgcttt gtagtagttc agaacgcctt cccaggttgc ccattgggta
     3241 tcggtatcga tgttcatttt cactacgccg tagcttacgg agtctttgat ttcctgagca
     3301 gtagaaccgg aaccaccgtg gaatacgaag ttcaggctgt tgtgcggcag gttgtgtttc
     3361 ttagaaacat attcctgaga atcacgcagg atggtcggag tcagaaccac gttacccggc
     3421 ttgtaaacac cgtgtacgtt accgaaggac gcagcgatgg tgaaacgcgg gctgattttg
     3481 ctcagctcgg tgtatgcgta atcaacgtct tccggctggg tgtacaatgc agaagcgtcc
     3541 atgtggctgt tgtccacgcc gtcttcttca ccaccggtgc aacccagttc gatttccaga
     3601 gtcatgccga ttttggacat acgctccagg tatttagagc agatttcgat gttctcttgc
     3661 agagattctt cagacaggtc gatcatgtga gaagagaaca gcggcttacc ggtagctgcg
     3721 aagtgttttt cacccgcgtc cagcagaccg tcgatccacg gcagcagttt cttcgcgcag
     3781 tggtcagtgt gcaggataac cggaacacca taatgttcag ccatctggtg aacgtgatgc
     3841 gcaccagaga tcgcgcccag gatagcagca ccctgcggaa cgtcagattt cacgccttta
     3901 ccagcgataa aggaagcacc accgttggag aactgaacga taaccggcgc tttaacttta
     3961 gcagcagttt ccagtacggc gttgatggag tcagtaccga cgcagtttac tgctggcagt
     4021 gcgaagttgt tttcttttgc tacctggaaa actttctgta cgtcatcacc agtgattacg
     4081 ccaggtttta cgaaatcaaa aatcttagac atgtctcttg tcctgtatcg tcgggcctta
     4141 tactcataat acttcaagtt gcatgtgctg cgtctgcgtt cgctcacccc agtcacttac
     4201 ttatgtaagc tcctggggat tcactctctt gtcgccttcc tgcaactcga attatttaga
     4261 gtatgaaaaa atgcgtgctc taaaagcgcg ctgaaacaag ggcaggtttc cctgccctgt
     4321 gattttttac ttcttagcgc gctcttcgag catcgctact gcaggcagta ctttaccttc
     4381 cacgaattcg aggaatgcgc cgccgccagt ggagatgtag gagattttgt cagcaatgcc
     4441 gaacaggtcg attgctgcca gagtgtcgcc gccgccagcg atggagaacg cttcgctgtc
     4501 tgcgatagcg ttagccacga tttcagtacc tttgcggaag ttcgggaatt cgaacacgcc
     4561 aaccggaccg ttccacagaa tggttttcgc attcttcagg atttcagcca gttcctgagc
     4621 ggaagcatca ccgatatcca ggatctgctc gtcagctttc acatcgttaa cagatttcag
     4681 ggtagccggt gcagtttcag agaactcggt tgctacgcga acatcagacg gaaccgggat
     4741 gttgcaggtg gtcagcagac gtttagcttc gtcaaccagg tcagcttcgt acagggattt
     4801 acccacatcg tggccttgtg ccgcgataaa ggtgttagcg ataccaccac caacaatcag
     4861 ctggtcagcg attttagaca gggagtccag aacggtcagt ttggtagata ctttagaacc
     4921 accaacgata gccaccatcg ggcgagcagg ttctttcagt gctttaccca gcgcgtccag
     4981 ttcagctgcc agcagcgggc ctgcacacgc aacgtcagcg aatttaccga taccgtgagt
     5041 agaagcctgc gcgcggtgag cagtaccgaa tgcgtccatt acgaacacgt cacacagtgc
     5101 agcgtatttt ttggacaggg tttcgtcgtc tttcttctcg cctttgttga agcgaacgtt
     5161 ttccagaaca accagttcac cttcagcaac gtcaacgccg tcgaggtaat ctttaaccag
     5221 acgaaccggg ttagacagtt tgtctttcag gtagttaaca accggcagca gagagaattc
     5281 ttcgttgtac tcgccttcgg taggacgacc caggtgggaa gttaccatca cttttgcgcc
     5341 ttgtttcagg gccagttcga tggtcggcag agaagcacgg atacgcgcgt cgctggttac
     5401 tttcccgtct tttactggta cgttcagatc cgcacggata aatacacgtt tcccagcaag
     5461 atccagatcg gtcatcttaa ttacagacat ggtgaatcct ctcgttgatt ctaaaagttt
     5521 tgcagacgct gcttgcgtct tacctgaaag caacagtagc catagctaac gtcgtgtcga
     5581 gcattcggtt agcaaagccc cattcgttat cgcaccagac caacgttttg atcaggtgtg
     5641 cgccactgac ccgggtttgg gtgccatcga caatggcact gtgcggatcg tggttaaaat
     5701 ctacagagac caacggcaat tccgtatagt caactatacc atgaaatgca ccttgtgctg
     5761 ctttttgcag caacaggttg acttcattgg cttttacagg tttcttcacc gtcacgctta
     5821 aatcgattgc cgtcacattt atggttggca cacgtaccgc aatcgcttca aagcgatcgt
     5881 taaattgcgg aaaaaatcgt gtgataccag cggccagttt agtatcgacc ggaatgatcg
     5941 actggctggc tgcccgggtg cgacgcaggt caggatggta tgcatcaata acctgttgat
     6001 cgtgcatggc ggagtgaatt gtggtcacag tgccggactc aataccgtac gcatcatcta
     6061 acaatttgat gacgggaatt atgcaattcg tggtacagga agcgttagaa acgatgcggt
     6121 gttccgcacg aagttgatcc tgattgacgc cgtaaacaac ggtcgcgtcg agatcgttac
     6181 tgccaggatg tgaaaagagc acttttttgg ccccggcggc aatatgcgct tcgccatgct
     6241 cgcgggagcc atatacgccg gtgcagtcga ggactacatc aacgccaagt tcacgccagg
     6301 ggagcgattg cagtgaacgt tcatgcaata cgcggatggc gtcatcacca acaaaaagtt
     6361 gatcgcgttc ctgtcgtact tcccatgcaa aacggccatg gctggtgtca tatttcaaca
     6421 aatgcgccat gcccgcagca tccgccagtt cgttgattgc caccacggta atttccgccc
     6481 ggcgtccgga ttcatacaaa gcacgaacca cattacgccc gatgcgaccg aagccattta
     6541 tcgctacgcg tacggtcata gatctcctgc aaggttttcc ctgagcaaat ttgccagaca
     6601 gagtaatcta gcaaatcgtc cggggaaacc ttacctgtcg caaactgcga ctgattggtt
     6661 aattgtcgaa catttaatcg actgaaacgc ttcagctagg ataagcgaaa cgtggaataa
     6721 aaggaatgat tgtccagtca aagaaagcaa ttatctgact tacgtcacgt tttcagcccc
     6781 gcccctgtgg aatttattcc actttgcaga atcacgaata agcgtagcaa aatcttttcg
     6841 ttgtgccgtc acaaaccctg acttatatca gaatatggct gatgcagcgt cgatattatc
     6901 cgcaatacgt ccgagtttcg gagtactcca atgatgacat tgccctaccc atattcttcc
     6961 tccgcacgct tttacgcact gcgcctcctc cccgggcagg aagttttttc ccagctacac
     7021 gcttttgcac agcaacatca acttcatgcc gcctggattg caggctgtac cggtagcctg
     7081 actgacgtcg ccttgcgcta tgccgggcaa gaaggcacca cgctcctgaa cggtacattc
     7141 gaagtgatct cactaaacgg cacgctggaa cagagtggtg agcatcttca tctctgcgtt
     7201 tccgatccac acggcacgat gcttggcggt catatgatgc ccggctgtac cgtgcggacc
     7261 acgcttgaac tggtgatcgg tagccttgag gaactggcgt ttagccgcca accttgcgcg
     7321 ctttctggtt acgacgagtt gcatatttcc cccgttaaat aactactttc aaagaggttt
     7381 tcttatggcg cgtagccact tttccagtca ggcactggtg ttgatcgtta tatctatcgc
     7441 catcaatatg atcggcgggc aactggccag catggtcaaa ttacctatat ttctcgactc
     7501 catcggcaca cttattagcg cggtactgct tggtccggtg atcggaatgc tgaccggctt
     7561 actgaccaat ttactctggg gattgctgac cgacccgatc gccgccgcat ttgcacccgt
     7621 cgcgatggtc atcggcctgg tctccggttg gctggcgcga gcgggttggt ttcgcaccct
     7681 gccaaaagtc gttgtcagcg gcgtgattat tacgcttgcg gtaacggtcg tcgctgtacc
     7741 gctgcgtact gcgctgttcg gcggtgtcac cggtagcgga gccgatttgt tcgttgcctg
     7801 gatgcactcg atggggcaaa acctggtgga gtcggtggcg attacggtga taggcgcgaa
     7861 cctggtcgat aaaattctta cagcggtgat tgtctggctt ttactgcgcc agctgccgat
     7921 tcgcaccacg cgccattttc cggcaatggc tgccgtgcgc taaatgcatc catttacctc
     7981 attaacactg tgggcattag cggcctgcac cacgcttatc ctgcccgcgc agaccatttt
     8041 gccgatctat agcgcggcga cctttttctg ccttattgcg ctaaaggcca cgcggcggcg
     8101 ggcaaaatat gtcgtctggc tgatgttctc gctgggagcc gggctgtggc tggtacacgg
     8161 tggctggcta ccagaatggc taagcggcac gccacgctcg ccagaacggt ggtcgcatgc
     8221 tattacgctc tggttgctga ttctggcgat tgtttcaacc tcacagttgt gaatgcagta
     8281 cgtccccgtg caatgtttta ttcgcgctct gtttgcttct cgcctgccgc caggcgtcgc
     8341 ttatctgttt gccgggccgt tattggtcgt tgagcagtta aaacggcaat tagcgattat
     8401 tcatgaagcg cagcgtgccc gcggcgtgcc gctggatgag ggctggtatc agcgtttacg
     8461 tgctatgccc gcgttaatta ttccgctaac acacaatgcg cttaacgatc tggctgtccg
     8521 tggtgcggcg ctggatatgc gggcatttcg gatccacaac cgacgaacaa cgttgtgggc
     8581 accagccgat agcaccctac aacgcgtagc gcgttacacc atgatcttgc tgatgctgac
     8641 agaattcgga gcgtggatat ggttacgtta gaacagttcc gttattgccc gacacactca
     8701 acgcatcctc cgttctgtta tgactttcac tatgtcaaac cggggatggt cgccatcttt
     8761 ggtgacaacg gcagtggcaa aagtacgctg gcacagctga tggcgggctg gtatccggac
     8821 tttctgccaa gagagataac aggcacgggg acgctcctcg gtacgcccat tgggcatctc
     8881 ccgcttaatg aacaatccgc caccatccag ctcgtccagc aatcgcccta tttgcagctc
     8941 tccggctgta cctttagcgt ggaagaagaa gttgcctttg ggccagaaaa tctgtgtctt
     9001 gctgaagcag agattatggc gcggattgat gccgccctga ctctgaccga gtgccagccg
     9061 ttgcgccatc gtcacccggc aacactctcc ggtggagaga cacagcgcgt ggtcatcgcc
     9121 tgcgccatcg cgatgcagcc aaagctctta attcttgatg aagcctttag ccgcctgacg
     9181 ccgcaggcca gcgaaatgct gttgcagcgc ttacaacact gggcgtttga acgtggttcg
     9241 ctgattattc tttttgaacg tcatcgcaca cctttcctga actactgcca gcaagcgtgg
     9301 caattgcaaa acggagcgct ccagccacta tgttaacgtt aaataagata tcttatcgct
     9361 ggcctggcgc ggcaacggat tgtctttgcg gcatttctct gcaactcaaa caaggtgaat
     9421 ggttggcgct gaccggtgac aatggcgcag ggaaatcgac gctgttacga gtaatggcag
     9481 gtcttcttac gcccacagcc ggtacggtta tgctgcaaca acaggcgatg aaaaacctga
     9541 aaaaccgcca gcgcgcggca aaggttggcg tactgtttca ggaagcggaa aaccaacttt
     9601 ttcacagcac cgtcgctgac gagatcgcat ttggtttgaa actgcaaaaa tgcccggcgg
     9661 atgaaatcac tcaacgtacc catgctgccc tgcaatgttg tcagttagca gacactgcca
     9721 actcgcaccc gttggatctg catagtgcgc agcgacggat ggtcgccgtc gcctgcctgg
     9781 aagcactctc tccgccacta ttactgctgg acgagccgag tcgggatttt gacgaaaatt
     9841 ggttgagcgt gtttgaaagc tggctggaga agtgccgcca gcgaggcacg agcgtggtag
     9901 caatcagtca tgacgccgca tttacccggc gtcatttctc tcgtgtggtg cgactggaag
     9961 atggtgtaat caggaacgtt aatccaccag acgatattca cccgtagcgg tcatcattaa
    10021 ggtcaaattt gcaggcagac tctcttccag cacccggcga acattcggcc cgtcggtacg
    10081 gtcataaaag gcttctgcat ctgccagcga tacccctccc gccagcttgc gccccaccag
    10141 acgctcccgc aacgcagcag ctggcgcgtt gataaagatc gagaaatcac aaaattgcgc
    10201 aagctggcac cacttctcgt cgtccagcaa caaccagttt ccttcgacga tgacgagtgg
    10261 tgcggtaacg tgcaacgcat cttcaacagg atcatgcttt tgtcgatcgt actgcggcca
    10321 cgtacaatcc ccttccacca cctggcgcag attttccgcc agtttcgcaa cgtcaaatgt
    10381 ctctggtgcg cctttgaagg ggcgcaattg atgcgcgtcc agccagctat tgtaatggtg
    10441 aaaaccatcc atcgggagcg tctgaatagc gggcagctcc gggtcttgtt gcgcgagata
    10501 ttcccagaag gtggttaggg tagatttacc cgtccccggc ggagcgcaaa gaaaaaccac
    10561 cgtacgccgc tgcggattta ccgtctgtaa ggccgccagc atacgcagca acggtttatg
    10621 cactcgttct atctcttcat catggtactg cgcttgcacg ttcagcccat tcaccgttaa
    10681 ttcaattttc acggcgttaa ttcctttaag gtggtcgtta ctgccagcgt cagcgccgtt
    10741 ttgaccattc cgctaaaacg cgcctctgac cagacggcga aatcggcgaa cttcatcgct
    10801 tttaacgcat cgtagagcgt cgcattgcga gttatactgt tgacattgct gataaaatcc
    10861 caggttatat catcggcaaa tccgggcgtt tcatcctgtt cgttcaggta atgccagtac
    10921 tgggaaaagt gcgtgatatc ggcatacagc catttatcca ttttccccag cgcataaatc
    10981 aaacgcagcg caacatcaat atcgtcaagc gggccgcttt gggcgagtaa cggcttcact
    11041 gcatattcga cgatctcttc atcgttatcg acaaacagcg acggcagaaa ctggcgtatc
    11101 ccctggagca aaatagaatg cgcagttttc ataaatgaaa ataaattgtc ctgtgcatcc
    11161 agttgctcaa gcacatcatc ttctgtcaga gtcgacattg gtcttccgat agatcaccat
    11221 caatgcaggg agtctattat attacacgtt tggtccgcgc cgccgatcag taatgtctgc
    11281 gtccgcaccc cattcgccgt ctgttggata ccgttcacca actcgccgcc cgtcacgccc
    11341 gtagcgctaa agaggatatc gttccccctc accagttcat cgagcgagta cacacggtta
    11401 acgtcaacgc ccattgcctt acaacgcttg cgctcctgct ctgcaatctg ccgattttcc
    11461 gtgtaatccc ctttagcctg acaaaagtca atcagctcgg cctgcatatc cccgcctaac
    11521 gctttaacgg cacaggcaga aatcacgcct tctggcgcac cgccgatggt gtacatcaca
    11581 tcgtaaggat tatcctgcca gcaggtcagc acgctggcgg cgacatcgcc atccggcagg
    11641 gcaaaaacct tcacgcccag ctgtgttgct tcttcaatcg cggcactcag acgcggttta
    11701 tccagcgtaa ccatgcgcag cttgtccagc ggtttaccta acgcccgcgc cacgttacgc
    11761 aagttgtccg ccagaggcaa agacagatca atcgccccgg cagccagtcg attaacaacc
    11821 agctttttca tatacatatc gggcgcatgt aacaggctat ctcgaggagc gaaagccatg
    11881 accgccagcg cattgctctg ccccatcgcc accatccgcg tgccttcaat gggatcaacc
    11941 gcgatatcga cttctggccc atcgcctttg cccacctctt cgccaatcca cagcatcggc
    12001 gcatggtcaa tttctccttc gcagataacc actcgtccac gaaaagcgac gtcgtttaat
    12061 gcctggcgca tcgcggtgac cgccaggcca tcaattttat ttttgtcgcc acatccggtt
    12121 tgcggccagg cagccaacgc ggcctgttcc gtaacgcgaa ataatggcca cgccagggac
    12181 atcattgcgc ggcctccccg atatcaacgc caaattgtgc cagcagatat ttctcggcct
    12241 gttcattcca gatgccgtgc gtttcctcaa ccagccgcgc caactcttta aacagcggat
    12301 cggttttgcc tttttcggca aaatcggcaa tggcagtgag cggcatggtc acgccgttat
    12361 aaatgagttt tttaccgcca gggatatccg gcagattgag cacggtttct ggcaccgcat
    12421 ccaggccacc aatatgggtc accataaacg acggctgtaa ctgtcctgtg gcgctaaggg
    12481 caatcgcctc tttcatgtcg tccgttgaac cgccggatgt accgactacg tgcgtgctgt
    12541 tgtaatggac gttgtagaaa ttaaacggca ctttgaagtt tttatccgtc agcccggcaa
    12601 agaagttcag acagccatct tccgccagta attcgtcagc catctcaacg acagcaggca
    12661 ccgccgcata aacaaaaatg tcatcgaacc cgacatctcc tgtcagcgcc cgcagcatct
    12721 ggataggatc gctcatccct ttggtattca catacaccag ctcaatgcct ttactggccg
    12781 ccagatccac cggcagcagc ttctgcacct gcgccaggcg tttgtcgtcg atatcgacca
    12841 ccaccacccg tgacggttgt atgccaccgt taatggcgta atcgatagcg ccaatgccca
    12901 tcggacccgc acacgccagc agtgcaatat tgccgccagg tttgacgccc atgcgatgct
    12961 cataaacata ttgcgtggtg tgataattgg catgataagc accaataatg cagcacatag
    13021 gctctgccag cgacgccgca gcaaaataag agccgtgata cggtaatacg cagcccaaat
    13081 taatggcgat ttcgggaata atcatataag tggcattgcc gccaaaatat tcgtagctgt
    13141 agcccgctga atatccgctt ggtaacccca tcgccggttg caatacaaaa cgctggcctt
    13201 ttttatattt gccagtgaga tttttaccca cttcgacaat aatcccggca cattcatgcc
    13261 cggtaatgac cggatgattt tctaaatcgt cgggtacgcg tttatgttca ctaccgagta
    13321 acgccgcttt ccaggtcgat aaacagacgc tgtcagaaat tacactcact aacagttcat
    13381 tatcggtaat ttctggcagt tcaaa
//
LOCUS       DADMEQ010000106        13311 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.107, whole genome shotgun sequence.
ACCESSION   DADMEQ010000106 DADMEQ010000000
VERSION     DADMEQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.107"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            72..392
                     /locus_tag="JNZ05_003092"
     CDS             72..392
                     /locus_tag="JNZ05_003092"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566234.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(458..690)
                     /locus_tag="JNZ05_003093"
                     /pseudo
     CDS             complement(458..690)
                     /locus_tag="JNZ05_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
     gene            complement(800..1570)
                     /gene="yafV"
                     /locus_tag="JNZ05_003094"
     CDS             complement(800..1570)
                     /gene="yafV"
                     /locus_tag="JNZ05_003094"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HAY8566235.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            1724..2197
                     /gene="ivy"
                     /locus_tag="JNZ05_003095"
     CDS             1724..2197
                     /gene="ivy"
                     /locus_tag="JNZ05_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-lysozyme inhibitor"
                     /protein_id="HAY8566236.1"
                     /translation="MGRISSGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTK
                     AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEK
                     SNQMTGLFSAIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(2240..4684)
                     /locus_tag="JNZ05_003096"
     CDS             complement(2240..4684)
                     /locus_tag="JNZ05_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAY8566237.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADSIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4923..5501
                     /gene="lpcA"
                     /locus_tag="JNZ05_003097"
     CDS             4923..5501
                     /gene="lpcA"
                     /locus_tag="JNZ05_003097"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HAY8566238.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5707..6474
                     /locus_tag="JNZ05_003098"
     CDS             5707..6474
                     /locus_tag="JNZ05_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HAY8566239.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(6445..7185)
                     /locus_tag="JNZ05_003099"
     CDS             complement(6445..7185)
                     /locus_tag="JNZ05_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HAY8566240.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            7477..8090
                     /locus_tag="JNZ05_003100"
                     /pseudo
     CDS             7477..8090
                     /locus_tag="JNZ05_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012599877.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            8266..8763
                     /gene="rayT"
                     /locus_tag="JNZ05_003101"
     CDS             8266..8763
                     /gene="rayT"
                     /locus_tag="JNZ05_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HAY8566241.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(8980..10719)
                     /gene="flhA"
                     /locus_tag="JNZ05_003102"
     CDS             complement(8980..10719)
                     /gene="flhA"
                     /locus_tag="JNZ05_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhA"
                     /protein_id="HAY8566242.1"
                     /translation="MLSRSDLLTLLTINFIVVTKEAERISEVSARFTLDAMPGKQMAI
                     DADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLSGGVC
                     IGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDVRH
                     QLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEKSL
                     ETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQVIS
                     DGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDGVL
                     GNDPAYGIPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQ
                     LHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSTVTKD
                     HILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQAGKVMLDSVP
                     VDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYNEV
                     PDELELKIMGALM"
     gene            10664..11449
                     /gene="lafU"
                     /locus_tag="JNZ05_003103"
     CDS             10664..11449
                     /gene="lafU"
                     /locus_tag="JNZ05_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HAY8566243.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            11520..12575
                     /gene="dinB"
                     /locus_tag="JNZ05_003104"
     CDS             11520..12575
                     /gene="dinB"
                     /locus_tag="JNZ05_003104"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAY8566244.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            12627..12920
                     /gene="yafN"
                     /locus_tag="JNZ05_003105"
     CDS             12627..12920
                     /gene="yafN"
                     /locus_tag="JNZ05_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HAY8566245.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            12923..>13311
                     /gene="yafO"
                     /locus_tag="JNZ05_003106"
     CDS             12923..>13311
                     /gene="yafO"
                     /locus_tag="JNZ05_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HAY8566246.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFR"
BASE COUNT         3229 a         3468 c         3434 g         3180 t
ORIGIN      
        1 gaggtgttag ttcatgggca aattcttgga aataaagtta gccgatgtaa ttagttagtg
       61 gagaggaagt tgtggacgaa attagaataa gaattgctgg taatacggct gcgccttgtt
      121 tccatgtgct aaaaaataag ggatataaag taacaatgga aactcatgca ttatctgacg
      181 attgtttaga ttgtgtatat gattggtatg cggaaaaaaa tggacaattt tttagtgcaa
      241 cgagtccaga agagttactt ggattagttg caatgtggga agttcgtggt aatgactgga
      301 gagctaattt gaatgagata aatacttatc aaaaagccat agaagaatcc ccgttttttg
      361 ataaaaacgg caatgaaata gagtctgact aaattttatt gatatagcaa cgataataac
      421 cggaaatttc ttccagatct ttccggcttt ttattattta actttcgttc agcacactaa
      481 acatcccgtc tcgcatcccc ttaactgact gtttaacctg ataaagttcc ctgcgcagtt
      541 cctgtacttc gttgttatcc caagcaggat caaacatccc tccgagatct tcacggtaac
      601 gcccgtgtcg atctcaaatc ccgcttttct cagccatttg gcgctgattt tgatcaccgg
      661 cgtgtcgctg cggttgggca gataacccac ggtacagcga cgctgtgttt tggaaatggt
      721 gacttctggc gtaatattgt gtttaagtta ggggctttcg taatctagcc cagcccccgg
      781 ccctaaacac aacccccact caccacaacc taaactcatc cgcatcccgc catgccggaa
      841 acttttctct atattcccgc aatgccacca tcgacagctc cgcatcaata cgcgttgcct
      901 tatgcgcgtc ggcggtagcg ataatctcgc cttgcggatt aatcacccgg ctgtcaccgc
      961 gataatggca gccgttgcca tcgctgccga cgcgattgca tcccgccaca tacgcctgat
     1021 tctcaatcgc gcgggccgtc agcaatgcct gccagtgcag agagcgcgga gctggccagt
     1081 tggcgacgta cagggcgagg tcataatcgt tgagattgcg cgaccacact ggaaaacgta
     1141 agtcgtagca caccagcggc aaaatacgcc agccgcgcca ttccacaatc actcgcgcat
     1201 tgcccgcttt ataatgcaga tgctcatctg ccatgcggaa cagatgacgc ttatcataaa
     1261 aatgtaccgt gccgcccggc tcaaccagca aaaagcggtt aaccgaacca gactccgttt
     1321 gtaatgcaac actgcctgca atcagcgcat tgcactgctg cgccttagct gtcatccagt
     1381 tcactacgtc atcttgtgca agcgacgaag ctgccgcttc catggcaaag ccgctggtaa
     1441 acatctccgg tagaacgatc acatcgcgcc cggtaatacc ttccagttga cgatcaaaat
     1501 ggcgcaggtt ggcaggacca tccacccaca ccaacggttg ctgcaaaagc gtaatcttca
     1561 aactaggcac ggtgtacaac tcctttatgc gaagggtttt ataactttaa caccttatca
     1621 ggcagttgcc ttagcgcaga ataaattgat aacaaatgct gatattggaa atatctgatt
     1681 tgcaaattat cgtgttatcg ccaggcttta ggaggttaat aacatgggca ggataagctc
     1741 gggaggaatg atgtttaagg caataacgac agtcgccgca ctggtcatcg ccaccagtgc
     1801 aatggcgcag gatgatttaa ccattagcag ccttgcaaag ggcgaaacca ccaaagctgc
     1861 gtttaatcag atggtgcaag ggcataagct acctgcatgg gtgatgaaag gcggtactta
     1921 tactcccgca caaaccgtaa cgttgggaga tgagacgtat caggtgatga gcgcgtgcaa
     1981 accgcatgac tgtggctcgc aacgtatcgc tgtgatgtgg tccgagaaat ctaatcagat
     2041 gacggggctg ttctcggcta ttgatgagaa aacgtcgcaa gagaaactca cctggctgaa
     2101 tgtgaacgat gcgctttcga ttgatggtaa aacggtgctg ttcgcggcgt tgaccggcag
     2161 cctggaaaac catccggatg gctttaattt taaataatta gcggataaag aaacggagcc
     2221 tttcggctcc gttattcaat tacgcggctt caactttccg cactttctcc ggcaacttta
     2281 ccggcttcgt cgccagctct tccggatcaa agtcatcaac gttaatactg cgcagacggc
     2341 tttcttccgc tttcaccaga atagcggctt catctttatc aatcagcccc ttcgtcagcg
     2401 cgttgtgcgc cagttcatcc agacgggtaa atggcagatt tttacccagc tctttacaga
     2461 tccgctgatg aatcgagtcg gcggcaatca catccaccaa cgcctcttcc agtaaaccaa
     2521 ccgggttatg ctcgcttggc gtcaggtact gaccgcgacc aatacgtgaa cgagtggcgt
     2581 tcggcacttg taaaatcttc gccactttat gatccagctt gtcagacggt gccagataat
     2641 gacgtccggt cgggaagatc accacattca gcagcccggc aaccacgcgg ttcgggaagt
     2701 tttgcagtaa atcgtccatc gcctgttctg cctgatacag cgcatcctgt acgccccagt
     2761 gcaccagcgg cagatcggct tcattacggc cttcgtcgtc ataacgcttc agaacggcag
     2821 aggcgaggta aagctggctt aaaatatccc ccagacgtgc cgagatacgc tcacgacgtt
     2881 tcaggctgcc gcccagcagt gccatcgaga catcagaaag cagggcgagg ttggcgctca
     2941 ggcggttcag gtgctgatag tagcgtttag tggcatcgcc ggttggcgtg ctgctggtta
     3001 aaccgcgcgt caggcccagc cagaagctgc gaactttgtt gctaccgacg tgaccgatat
     3061 gtttgaacaa cagtttatcg aacgcgttga cgtcattgtt cttcgccgct tccatctctt
     3121 ccagcacgta cggatggcaa cgaatcgctc cttgtccgaa gatcatcatg ctgcgggtca
     3181 gaatgttagc cccttcaacg gtgatggcaa tcggtgcgcc ctggtaagca cgcgccagga
     3241 agttgctttg cccgagcata atgcctttac cgccggtaat atccatcgca tcaataatcg
     3301 actgctgccc gcggtgggta cagtgatact taacgatagc cgacagcacg gcaggttttt
     3361 cgccgagcat aatgccgtag gtaatcagcg atgccgcagc atccattaca taagcgttcc
     3421 cggcaatacg cgccagcggc tcttcaatcc cttccatctt accgatagag attttgaact
     3481 gacggcgaat gtgagaatac gcgcccgttg ccagcgctac cgatttcacg ccgccggttg
     3541 agttggaagg cagggtgatg ccgcggccta ccgagaggca ctccaccagc atccgccagc
     3601 cttgcccggc cattttcggc ccgccgatta tgtaatcgat cggcacgaag acatctttac
     3661 cgcgcgtcgg tccgttctgg aacggtacgt tcagcgggaa gtggcgacga ccaatttcca
     3721 cgcccggcgt ggtggttggg atcagcgcac aggtaatgcc taaatcttct gcaccgccga
     3781 gtaatttttc cgggtcggag agtttaaacg ccagcccaag cacggtcgca atcggtgcca
     3841 gcgtaatgta gcgtttgttc caggtcagac gcatccccag cacctgctgg ccctgccatt
     3901 cgcccatgca gacaatcccg gtgtccggaa tcgcgcccgc atcggaaccc gcttccgggc
     3961 tggtcagtgc aaagcagggg atctcctgac cacgcgccag acgcggcaga tagtgatctt
     4021 tctgctcgtc agtgccgtaa tgttgcaaca gttcgcccgg gcctaatgag tttggcacgc
     4081 cgacggtaat cgccaggatc ccgctcacgc cagagagttt ttgcagcaca cgagactggg
     4141 cataagctga gaactccagt ccgccgtact cttttttgat aatcatcgcg aagaagcgat
     4201 gctctttaag gtacgcccac aactccggcg gcagatccgc cagctcatga gtaatctgga
     4261 aatcattcgc catccggcag gcttcttcta ccgggccgtc gagaaacgct tgctcttcag
     4321 cggtcaggcg cggctgcgga tagttatgca gctttttcca gtccggcttg ccctggaaca
     4381 agtcgccctc ccaccaggtg gtgcccgcat caatcgcttc tttctcagtg cgcgacatcg
     4441 gcggcatcac cttacggaaa ccgcgaaata ccggcgcgga aatcatcgac ttacgcatag
     4501 gcgcaaagtt aaatggcacg aggataatgg tcagaggcac cagtacccac gccgaccaca
     4561 gaccagcaac gccgagggcg gctgtccagg cgagcaaaat cagactgctg ataaataagc
     4621 tcacgcggtg atagaacaac gcgccgagca ggacaaccgt agcgagaata ctcaaaatca
     4681 tcataacgaa aagcccctta cttgtaggag gtctgaccac tgtgatgata tggttgtagt
     4741 ggatgtaaaa acatttagca atgtgtttac aatataatta caacaaagct cacattgttg
     4801 ctgtttttat ccgcacttca ggtcaaaaag tcctggtcat agcacctgcc cgtacttctc
     4861 gcttttggcg gtatccggta cactgcattt tgtctattac atttatgctg aaggatatcc
     4921 tcatgtacca ggatcttatt cgtaacgaac tgaacgaagc ggcggaaacg ctggctaact
     4981 ttttaaaaga tgacgccaat attcacgcca ttcagcgcgc ggcggtcctg ttagcagaca
     5041 gctttaaagc cggtggcaaa gtgctttcct gcggcaacgg cggttcccat tgcgacgcta
     5101 tgcactttgc cgaagagttg accggtcgct accgtgaaaa ccgtccgggc tacccggcga
     5161 ttgctatttc tgacgttagt catatttcct gcgtcggtaa tgatttcggt ttcaatgata
     5221 ttttctcccg ctacgttgaa gcggtaggtc gcgaaggcga tgtactgctg gggatctcca
     5281 cctccggtaa ctctgcaaac gtgatcaaag cgatcgcagc ggcgcgtgag aaggggatga
     5341 aagtgatcac cctgaccggt aaagacggcg gtaaaatggc tggcacggcg gatatcgaaa
     5401 ttcgcgttcc gcactttggt tatgccgacc gcattcagga gattcacatt aaagtgatcc
     5461 atatcctgat ccagttaatt gaaaaagaga tggttaagta agtctggcgt aggccggata
     5521 aggcgtttac gccgcatccg gcacttgtgc gctgatgcct gatgcgatgc tgacgcgtct
     5581 tatcatgcct acaaatctgt acccgaaccg taggccgaat aattcgttta cgccgcatcc
     5641 gacctgaaaa ttcttaaatc aatcttcgcc ggggtccatg cgctcccgct gttgtggagg
     5701 ttacccatgt gcgaattgct cgggatgagc gccaacgtcc ctaccgatat ctgctttagt
     5761 ttcaccggac ttgtacagcg tggtggtgga accgggccac ataaagatgg ctggggcatt
     5821 accttttacg aaggtaaagg ctgtcgcaca tttaaagatc cacaacccag ctttaattcc
     5881 cccatcgcca aacttgtcca ggactacccg ataaaatcct gttcggtggt ggctcatatt
     5941 cgccaggcta atcggggcga ggtggcgctg gaaaatactc acccatttac ccgcgagtta
     6001 tgggggcgta actggactta tgcccataac ggacaactga cgggctacaa atcactggaa
     6061 accggcaact tccgcccggt cggtgaaacc gacagcgaaa aagccttttg ctggctgctg
     6121 cataaattaa cgcagcgtta cccgcgcacg ccgggcaaca tggcggcagt gtttaaatat
     6181 atcgcctcac tggcggatga actgcgggcg aagggcgttt tcaacatgct gctttcggac
     6241 gggcgctatg taatggcgta ttgctcgact aatttacact ggatcacccg ccgcgcgccg
     6301 tttggcgtgg caacgttgct ggatcaggat gtggaaatcg acttcagctc gcagaccaca
     6361 ccgaatgatg tggtcacggt gattgcgaca cagccgctga cgggcaatga aacctggcaa
     6421 aaaattatgc caggcgaatg gcgcttattt tgcctcgggg agcgtgtagt ttgatgccag
     6481 ttgtggctgc accacttcgt ggcttaacgg cttgctgacc acgtaacggc cattggccac
     6541 agaaacggtc ggtggcttac gggtttgctc aaagtagtcg tagcccggct tcagctgctc
     6601 ccagaagtcc ttaaagttgg aatatttatg gcgcttcata ttggcgtcgg tcatgcggaa
     6661 tgggtaaata ctcacctgca cgctcggctg accaaacacc agcgcaccag taacgaactg
     6721 gaagatctca tcaataccct gattggtcat tgcgtagcag ccgatggata cacaatcgcc
     6781 gtggatcatc aggtatttcc cttcataacc atgcgcacgg tcataagcat tggggaatcc
     6841 aatattaatc gctttgtagt aacggctgtc tggttttaac tgattacgct ggacgctata
     6901 aaatccttcc gggcttttga aatcgccctg acgctgtttt ggccctaagc cgccggaata
     6961 tttacagatt ttataactgt cgagcagttg atattgctcg cccattttga cgtaaagatc
     7021 gagcgtacgt tcttccttga agatctggat gtagacaggg gatcccatca actgctgctt
     7081 atactctttg ctcactggcg tggtcgagct actgctgccc agcaaaccgg caaacgaaac
     7141 gcacgggatc aacaacatcg caagaattaa tgcgatttta cgcatactgc ttattccttg
     7201 ataaaacggt tacacacgcc aggacggcaa aatggatccc aaatcggaat agtctggatt
     7261 tggaaggctc acattatcac caaaagagtt ttacgcaagc ctgtcggcgc tgggtttaca
     7321 aatttagcac aggggcagtt ttcaatagtc cgaaaagcgc atgtatcatt tatttcctaa
     7381 tattatgaaa tctcaatagt aatatgcaaa taagcaatta ataatcccgt ttactcctcc
     7441 ggtcttttaa aattctcacc ttcattcagc cggtttatgt ctttcatgtc ttcgttcttg
     7501 ctcggcagat ttttgcatcc aggcgtgttt tcgttatgcg ttttgctccc tttactcgcc
     7561 agcgccacca caaccgggag cagataaaac agcatccaga atggtttcct gcgccgctca
     7621 aggcgagtga cagacgctgg caggagctgg cggaaaacaa ccacttttta agcagcgatc
     7681 acctgcataa catcaccgaa gtggcgattc accgcctgga gcagcagctt ggcaagcctt
     7741 acgtctgggg cggcacgcgg cccgatcaag gcttcgattg tagtgggtta gttttttatg
     7801 cctacaacaa gatccttgag gcgaaactcc cgcgcacggc caatgagatg taccactacc
     7861 gtcgggcaac gattgtggcg aataacgacc tgcgccgggg agatttgcta tttttccata
     7921 tccacagccg cgagattgcc gaccatatgg gggtgtatct gggcgacggt cagtttattg
     7981 agtcactgcg caccggggaa accattcggg taagccggtt agccgaacct ttctggcagg
     8041 accatttttt aggcgcacgc aggattttga cggaagagat gattttgtag gccggataag
     8101 gcgtttacgc cgcatccggc ggttgtgcac ggatgcctga tgcgacgctg acgcgtctta
     8161 tcatgcctac aaatctgtgc gtttttgcga gccgctttcc cgatataaca cagcgtcatt
     8221 gtcggccttt ccttttcctc ttaccgttga gaaaaaggag tcgctatgtc ggaatatcgt
     8281 cgttattaca tcaagggggg aacatggttt ttcacggtaa atttacgcaa tcgtcgaagc
     8341 catcttctga ccacccagtt ccagacgctc cgtaacgcca ttattaacgt taagcgagac
     8401 agaccttttg aaattaacgc ctgggtcgtt ttgccggagc atatgcattg catatggaca
     8461 ttacccgaaa gcgatgatga tttttcctct cgctggcggg agattaaaaa gcagttcacc
     8521 catgcttgcg gattgaaaaa tatctggcaa ccgcgttttt gggagcatgc cattcgcaat
     8581 acaaaagatt atcgccatca tgttgattat atttatataa atccagagaa acatggctgg
     8641 gtaaagcagg tgagtgattg gccattctca acgttccatc gcgatgtcgc gcgagggtta
     8701 tatcccatcg attgggcggg agacataacg gatttaagtg ccggggagcg tatcatttta
     8761 taactgtgcg ctggtgccgg atgcgcttac gcttatccgg cctacaaatc gtgctttaac
     8821 ggtaggccgg ataaggcgtt cacgccgcat ccggcagtta tgcgcagatg cctgatgcga
     8881 cgctggcgcg tcttatcatg cctaaaaact tgtgcctgaa cggtaggccg gataaggcgt
     8941 ttacgccgca tccggcaatt gtgcaccgat gccggattct tacatcaacg cccccataat
     9001 cttcaactcc agctcatccg gcacttcgtt atacgacagc acatgcagcc ccggcgcaaa
     9061 caaccttgca taacgcgcca gcaaagggcg tagctgcggc ggcaccagca gcaccggatc
     9121 tttccccgcc gctttcatct gctctttcac ctgtggcatc gtgctctgga actggttgag
     9181 catattcggg tcaaccggca cgctgtcgag catcactttg ccggcctgtt gcgcctgatt
     9241 cactacgttg gtcagcagat tttccagctc attattcagc gtatacaccg tcagctcctg
     9301 cttgcgaacg aacggatgag taatgctgcg ccgcagcgcc aggcgcacat cggccgccag
     9361 cagaatatga tctttagtca ccgtgctact ggcgaccagc acggtggcga tggtgacgat
     9421 atcgcgcagg gaaacgcctt cggtcagcag cgcccggtac actttcagca attggctgta
     9481 attgagcgcc gcgcttaaat cctccgccag gcgcggtgcc atcgacgaca aacggttatg
     9541 caactgcgtg atgtcatcat agttaaacaa atcaggaata tagctgcgca caatcttgtt
     9601 cacatgggtg gcaatcacac tggcgctgtc gatcacctga taccccatat tcagagcctt
     9661 cgctttctgc gcaggctgga tccaggtgac cggtatcccg tacgccgggt cgttccccag
     9721 cacgccgtca atctcgccgt aggtttcgct ggagggcagc gccatcagtt tatccgccgg
     9781 aatatccgct tcatcagcct taatgccgtt gatgaaaata gcgtactggc tgggcttgag
     9841 gcggaagttt tcccgaatgc ggatctccgg cagcagcacg ccgttaccgt cggaaatcac
     9901 ctgccgaacg ccgcgaatcc gctgggtgag cgggttgccc tgcgctttgt ccaccagcgc
     9961 caccagcttg tagccgaggc ttaagctgat gggctcgatc agcggaatag tttcccaact
    10021 gacctgttgt tcgctggttt cagtgatggt gcgggtcagc gtttcgaggc ttttctcttc
    10081 cgcctccgcc gcctgcgggc gtttgctcat ccgccagccg gtaaagccaa gcagggcgct
    10141 gaacagcaaa aacggcagat gcggcatccc cggcaccacc gccagcacga acatgatccc
    10201 ggtggcggtg tagagcaccg acgggcttgc cagcaactgg tggcgcacgt catgggcgat
    10261 atcgccgttg tcgctgacgc gggtgacgat aatcgccgcc gcggtggaga gcagcaggga
    10321 agggatctgc gccaccaggc cgtcgccgat ggtcatcagc acgtattgct ggaaggcggc
    10381 gtcggcgctc aggttgtatt tgaagatgcc gatacagacg ccgccgctca ggttgatcgc
    10441 cagaatcatc atcccggcga tggcgtctcc gcgtacaaac ttcgatgccc cgtccatcgc
    10501 gccgtagaaa tcggcctcgc tggcaacatc tttacgccgg gtttgcgcct gcgcctggtt
    10561 gatcaatccg gcgttaagat cggcgtcaat cgccatctgt ttgccgggca tcgcgtccag
    10621 ggtaaagcgg gcagaaacct cggaaatacg ctcggcctct ttggtgacga cgataaagtt
    10681 gattgtcaac agcgtcagta aatccgagcg tgaaagcatt atcgccgcgc tgcacgggca
    10741 gtcgattttt aacggtggtg ggctgtcgcc gttaaataaa atcagcccgt ctcatccgcc
    10801 aaaacctgcc accgtggcgg tgccggaaga gacggagaaa aaagcgcgtg atgtgaatga
    10861 gaaaacggcg ctgctgaaga agaaatccgc caccgagctt ggcgagttgg caaccagtat
    10921 caacaccatt gcccgtgatg cgcatatgga agcgaatctg gagatggaga ttgttcccca
    10981 gggattacgc gtgctgatta aagacgacca gaatcgcaat atgttcgaac gcggcagcgc
    11041 gcagattatg ccgttcttta aaactttgct ggtggagctc gcgccagtgt tcgactcgct
    11101 ctataacaaa attattatta ccgggcatac cgatgcgatg gcctacaaaa acaatatcta
    11161 caacaactgg aacctttcgg gcgatcgcgc gctttcagct cgtcgggtgc tggaagaggc
    11221 cggaatgccg gaagataaag tgatgcaggt aagcgcaatg gcggaccaga tgctgctgga
    11281 cgcgaagaat ccgcaaagcg cgggcaatcg gcgtattgag attatggtac tgaccaaaag
    11341 tgcgtccgat acgctgtatc aatactttgg tcagcatggg gataaagtgg tgcagccgct
    11401 ggtgcaaaag ctggataagc agcaggtgct ttcgcagcga atgcgctaaa tgctgaatct
    11461 ttacgcattt ctcaaaacct gaaatcactg tatactttac cagtgttgag aggtgagcaa
    11521 tgcgtaaaat cattcatgtg gatatggact gctttttcgc agcggtggag atgcgcgaca
    11581 atcccgctct gcgcgatatc cctattgcta ttggcggcag ccgcgaacgt cggggggtga
    11641 tcagcaccgc caattatccc gcgcgtaaat ttggcgtacg tagcgctatg cctactggga
    11701 tggcgctcaa attatgccca catctcacct tgcttccggg gcgctttgac gcctacaaag
    11761 aagcctcaaa tcatatccgt gaaatcttct cgcgctacac ctcgcgcatt gaaccgttgt
    11821 cactggatga ggcttatctc gatgtcaccg atagcgtcca ttgccacggt tctgcgaccc
    11881 tcatcgccca ggaaatccgc cagacgatct tcaacgagct gcaactgacg gcatctgcgg
    11941 gcgtggcacc agtaaagttt ctcgccaaaa tcgcctccga catgaataaa cccaacggtc
    12001 agtttgtgat tacgccggca gaagttccgg catttttaca aaccttacca ctggaaaaaa
    12061 tccccggcgt cggcaaagtc tcggcggcaa aactggaagc gatggggctg cggacctgcg
    12121 gtgatgtaca aaagtgtgat ctggtgattc tgcttaaacg ctttggcaaa tttggccgca
    12181 ttttgtggga gcgtagtcag gggattgacg agcgcgacgt taacagcgaa cggttacgaa
    12241 aatccgtcgg cgtggaacgc acgatggcgg aagatattca tcactggtct gaatgtgaag
    12301 cgattatcga gcggctgtat ccggaacttg aacgccgtct ggcaaaggta aaacctgatt
    12361 tactgattgc tcgccagggg gtgaaattaa agttcgacga ttttcagcag accacccagg
    12421 agcacgtctg gccgcggctg aataaagctg acttaatcgc caccgcgcgt aaaacctggg
    12481 atgaacgccg cggcgggcgc ggtgtgcgtc tggtggggct gcatgtgacg ttgcttgacc
    12541 cgcaaatgga aagacaactg gtgctgggat tatgatgtat actattatgt atattctggt
    12601 gtgcattatt atgagggtat cactgtatgc atcggattct cgctgaaaaa tcggtcaata
    12661 tcactgagtt acgtaaaaac ccagctaaat actttattga tcaaccagtt gcggttcttt
    12721 ctaataatcg ccccgcagga tatctcttaa gtgccagcgc attcgaagcg ttaatggaca
    12781 tgcttgctga acaagaggag aaaaagccca taaaggcgcg ctttcgtcca agtgctgcaa
    12841 gattagagga aattacacgc cgcgctaaac aatatcttaa tgatatgacg gatgatgatt
    12901 tcaatgactt taaggaataa ggatgcgggt attcaaaaca aaacttattc gcctgcaact
    12961 tacagcagag gaacttgatg cgttaacggc ggattttatt tcctataagc gtgacggtgt
    13021 tttgccagat atatttggtc gcgatgcact ctacgacgac tcgtttacct ggccattaat
    13081 caaatttgag cgagttgctc atattcatct ggcaaatgtg aataatccat ttccgccaca
    13141 gttgcgccaa ttcagcagaa cgaatgacga agcgcatttg gtatattgtc agggtgcgtt
    13201 tgatgagcaa gcatggttgc tcattgccat tctgaagcct gaacctcata aactggctcg
    13261 agataacaac caaatgcata aaattggaaa aatggcagaa gcgtttcgca t
//
LOCUS       DADMEQ010000107        13277 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.108, whole genome shotgun sequence.
ACCESSION   DADMEQ010000107 DADMEQ010000000
VERSION     DADMEQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.108"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            34..435
                     /locus_tag="JNZ05_003107"
     CDS             34..435
                     /locus_tag="JNZ05_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HAY8566247.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVSAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="JNZ05_003108"
     CDS             447..818
                     /locus_tag="JNZ05_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8566248.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..1395
                     /locus_tag="JNZ05_003109"
     CDS             805..1395
                     /locus_tag="JNZ05_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8566249.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            1392..1791
                     /locus_tag="JNZ05_003110"
                     /pseudo
     CDS             1392..1791
                     /locus_tag="JNZ05_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1802..2542
                     /locus_tag="JNZ05_003111"
     CDS             1802..2542
                     /locus_tag="JNZ05_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8566250.1"
                     /translation="MTTPNPLAKTKGAGTTLWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYLNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVPLSGLVATVKG
                     VQQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            2601..2987
                     /locus_tag="JNZ05_003112"
     CDS             2601..2987
                     /locus_tag="JNZ05_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HAY8566251.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRKAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2996..3325
                     /locus_tag="JNZ05_003113"
     CDS             2996..3325
                     /locus_tag="JNZ05_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HAY8566252.1"
                     /translation="MQFVMRLAREFRRADWRQMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            3297..5240
                     /locus_tag="JNZ05_003114"
                     /pseudo
     CDS             3297..5240
                     /locus_tag="JNZ05_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            5237..6338
                     /locus_tag="JNZ05_003115"
                     /pseudo
     CDS             5237..6338
                     /locus_tag="JNZ05_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098299.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            6338..6667
                     /locus_tag="JNZ05_003116"
     CDS             6338..6667
                     /locus_tag="JNZ05_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8566253.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIREVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            6667..7365
                     /locus_tag="JNZ05_003117"
     CDS             6667..7365
                     /locus_tag="JNZ05_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAY8566254.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGAGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            7370..8113
                     /locus_tag="JNZ05_003118"
                     /pseudo
     CDS             7370..8113
                     /locus_tag="JNZ05_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            8050..8651
                     /locus_tag="JNZ05_003119"
                     /pseudo
     CDS             8050..8651
                     /locus_tag="JNZ05_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            8712..12191
                     /locus_tag="JNZ05_003120"
                     /pseudo
     CDS             8712..12191
                     /locus_tag="JNZ05_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            12259..12858
                     /locus_tag="JNZ05_003121"
     CDS             12259..12858
                     /locus_tag="JNZ05_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAY8566255.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQPHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            12922..>13277
                     /locus_tag="JNZ05_003122"
     CDS             12922..>13277
                     /locus_tag="JNZ05_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8566256.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGA"
BASE COUNT         3118 a         3123 c         4242 g         2794 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgctca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctctgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccgctgtc cggaccgctg acacaggcat ttgaagatgc ccgcgatcgc
     1321 atcattgctg cggaaatgcc gaaacagctg gggtatgccc tgaaacaaca actgaggtta
     1381 catctgagta aatgaaccgg cacacacaaa tccgtcagag cgtgctggca cgccttcggg
     1441 aacagtgtgg agacagcgcc acgttttttg acgggcttcc ggcatttatt gatgcgcaaa
     1501 ctgcctgccg tggcggtgtg gctgagtgat gctcagtaca ccggaaaaat gacggatgga
     1561 gatgactggc aggctgttct gtatattgcc gtcttcatcc gggcacaggc accggattca
     1621 gagctggata tgtggatgga gagcaccatt ttcccggccc tgaatgatgt accggcactt
     1681 tccggactca tcgacaccct gaacccactc ggttttaact atcaacgtga taatgagatg
     1741 gctacctggg cgatggcgga aatcacgtac cagatcacgt acacgaatta aggaggtggt
     1801 aatgaccaca ccaaatccac tggcaaaaac gaaaggtgcg ggaacgacgt tatggatgta
     1861 taccggcaac ggcgacgcat ttgcgaaccc tttgtcggac actgactggc tgcgtcttgc
     1921 gatggtgaag gatctgcaac ctggcgagat gaccgctgat gcagaagatg acacttatct
     1981 cgatgatgaa gatgcagact ggaaaacgac aacccagggg cagaaatccg tcggtgatac
     2041 ttcggcgacg ctggcctggc gtccgggtga cagcgggcag aaaaaactgg ttcagttgtt
     2101 cgattccggt gaagtctgcg cgtttcgtat caaatacctc aacggtacgg ttgatgtttt
     2161 ccgtggctgg ctgagttcac tgggtaaaac cattacctca aaagaggtga tgacccgtac
     2221 tgtaaaaatc agcggtgtgg ggcgtccgta tctggcagag gagggcgctg aaaccgtggg
     2281 tgttaccgga ctgacggtgg caccggcatc tgtcagtgtc aaagcgggag caaccaccac
     2341 actgaccttt acagtaaaac ctgacggggc cagtgacaaa gcgatcagtg tgcattcgtc
     2401 agatcctcag actgcttcgg tgcccctgag cgggcttgtg gccacggtga aaggcgtgca
     2461 gcagggcagt gtcagcatcg tgggcatgac ctctgacgga gagtttgtgg cagtggctgc
     2521 ggtgaccgtc agcgcaccat aacaggacga tactcatcat tgccccggtt atccggggct
     2581 ttttgcatcc ggagaacatg atgtttctga aacaggatac gtttaattat gaaaaacagt
     2641 ccgtggtgct cagtgagctg tccgggctgc agagaattga atatctgacg tttgttcagc
     2701 agcgaaaggc aaagtttgat gcacaggagg gagaactgcc ggaggctgaa cgacagattg
     2761 cttttctgcg tatgggaatg gatatcaatg cctggctggt ttcccgctca ctgtggaatg
     2821 ctgagcagtc tcaggatgtt gagacgcttt gcgcatccat tatgacaaca tggtcgtatg
     2881 atgcgctggg cgcgggggcg gagagggttc tgtcgctgag cggtatgggg accattgaga
     2941 atgccgggga tgatgatcat gaggcgctga cgccggaaaa gtcctgacgc gggaaatgca
     3001 gtttgtcatg cggcttgccc gggagttccg gcgggcagac tggcggcaga tgctgtcgga
     3061 tatgtcggcc actgagcttg gtgagtgggg cgattatttc cggatgcaga gcttcagtga
     3121 tgtgtggatg gatgcgcagt ttgcctcgct gaaggcattg atcgtgagaa tggtgtccgg
     3181 cagcagcgat gctgcggtgg ctgatttcag ccttttaccg gaagagaacg ggataccgga
     3241 gcgaacggac gaagaactga tgcatcttgg ggaaggtatt tccggaggtg tgcgttatgg
     3301 accagatagc caacctggtc attgatttgg ggattgatgc ggcagagttt aaaaatgaaa
     3361 ttccccgtat caaaaacctt ctgaatggtg cagccagcga tgcagaacgg tcttctgccc
     3421 gtatgcagcg ttttatggag cgtcagactc aggcggcccg gcagacaatg caggcggctt
     3481 cttcggctgc aacagcagca tcagcccatg cgcagacggt ggagaagaat gcacgggctc
     3541 atgaacgcat ggcccgtgag gtggaacaaa cccgtctgcg cgtggatgcc ctgaatcaga
     3601 aaatgcgcga ggaacaggcg caggccaggg cactggcgga ggcgcaggat aaagcggctg
     3661 ccgcctttta tcgccagatt gacagtgtga aacaggccgg tgcggggctt caggaattac
     3721 agcgtattca gcagcagatc cgacaggcca gaaacagtgg cggggttggt cagcaggatt
     3781 atctggcgct gatttcggag atcacggcga aaacccgtgc cctgacgcag gcagaggaac
     3841 aggccacccg gcagaaagcg gcgtttatcc gccagcttaa agagcaggca acccgccaga
     3901 atctgtcgtc ttctgagttg cttcgcgcca gggcggctca gctgggggta agcagtgctg
     3961 cagaagtgta tatccgcaaa atggagcggg caggaaaagc cacacattcg ctggggctga
     4021 aaagtgcggc agcccggcgg gagctggggg tgttaatcag tcagatggcg cgcggcaatt
     4081 ttggtgcgct gaggggatcc gggataacgc tggctaaccg tgccggatgg acaggcgcac
     4141 tgatgtcgcc gaaaggcatg atgactggcg gcgttattgg cggacttgtc gcggcggtcc
     4201 tgggtctggg taaagcctgg catgacggcg gaaggagggc gaggaattta accgtcagct
     4261 ggcgctgacg ggacattatg ccggtgtcac tgtcgggcag ttgtggaaac tcagccgggc
     4321 catatccggg aatggtatca cgcaacatgc ggcagccggt gcgctggcac aggtggtggg
     4381 cagtggggca tttcatggaa acgatattgg tatggtggcg aaagctgccg cacagatgga
     4441 gcgatcggtt ggccagtcgg tcagcgatac cataaatcag tttaagcggc tgaaggatga
     4501 tcctgtaaat gccgcgaagg cgctggacaa tgcgctgcat tttttgactg ccactcagct
     4561 tgagcagata cgcgtccttg gggagcaggg gcggtccagt gatgcggccc ggatcgccat
     4621 gtctgcgctg gcagaggaaa ccggtaaacg cacatctgat attgataata atctcaatgc
     4681 gctgggtagt acgctgcaaa ccttgtctga ctggtggaag cagttctggg atgcggccat
     4741 gaatattggt cgtgaagact cgctggatgc gcagattgat gcgttacagg aaaaaattca
     4801 gcgcgcgaaa aaatatccgt ggacaaacgc ctccacacag gtggagtacg atcagcagcg
     4861 tcttaacgat cttcaggaga aaaaacgccg gaaggatttg caggatgcaa aagcgcaggc
     4921 agaacggaat taccaggagc aacagaaacg ccggaatgct gaaaatgccg cgctgaaccg
     4981 gatgaatgaa acggaagctg cacgacatca gcgggaaatt gcgcgtatta atgccatgca
     5041 gtacgccgac caggctgtca gggatgcggc gatacagcgt gaaaatgaac gttacgaaaa
     5101 agccattaag aaaaatacac gggcaacccg taatgatgag gccacccggt tattgctgca
     5161 gtacagtcag cagcaggcac aggtggaagg gcagattgct gctgccagac agtcagcagg
     5221 cattgccact gaaaggatga ctgaagcgca taaacagctt ctggccctgc agcagcgcat
     5281 cagcgacctg gacgggaaaa agctgacggc agatgaaaag agtgtgctgg cccgtaagaa
     5341 tgagctgatt caggcgctga cgctgctgga tgtgaaacag caggagctgc agaaacagac
     5401 agcgcttaac gacctgagaa aaaaaacggt tcagctgacc agccagctgg cagacaaaga
     5461 acgtgcactg cgtgagcagc acaatctgga tattgccact gcaggtatgg gggataagca
     5521 gcggcagcgc taccaggcac agttgcgcat ccggcaggaa taccggcaac agttgcaaca
     5581 gcttgagaat gacagtcgcc agaaaggcac ttacgggacg gaggactacc ggagggctga
     5641 ggaggtgctg aaggggagcc tgaagcgaca actgaatgaa aacaaacgct actggcagga
     5701 actggaagtg gcgcagggcg actggaaaaa cggtgccatg cgggcgtttc agaattttac
     5761 ggcggatgcg gataatgcgg cgggaacggc agaacagatg tttacagtgg cattcagcag
     5821 tgccggtaat gcactggcga cattctgtac caccggtaag ctgaatttta aatccttcac
     5881 ctcttccctg ttgtcagata tggccagaat tatggcacag atggccatga tgcaggcggt
     5941 aaagggcgtc ggttctttat tcggcttcac gactaatgct gatggcggtg tttaccagtc
     6001 tgctgatttg agtcgctaca gtggcacggt ggttaaccgt ccgacgtttt ttgcttttgc
     6061 aaaaggcgcg ggtgtgatgg gggaagcggg acctgaagcc attctgccac tgcgtcgcgg
     6121 tgctgatggt aagctggggg gttgtggcgg atactggtgg ttcaggcatg gtgatgtttg
     6181 ccccgcagta caacattgag atcaataacg acggcacgaa cgggcagata ggtccggctg
     6241 ccctgaaggt ggtttatgac ctcgggaaaa aagcggccgc ggactttatg caacagcagg
     6301 cccgtgatgg tggccggtta agtggagcat accggtaatg gagacgtttc actggaaagt
     6361 gcgcccggat atgaatgtgg tatcagagcc gaaagtggtg acagtgaagc tgggcgatgg
     6421 ttatgaacag cgtcgtgcgg cgggactgaa taaccagttg tcgacttaca gcgtgacgat
     6481 acgtgttcgt aaaggtgaac acccatcttt aaaagccttt ctggaacggc acggtggcgt
     6541 ccgcgcattt cagtggacgc caccttatga ctggaaactg atcagggagg tttgtcgtaa
     6601 atggtcggca agcgtggggg cgttatgggt gactgtaacg gccgattttg aacaggtggt
     6661 gaactgatgc aggatatccg gcaggaaaca ctgaatgaat gcacccgtgc ggagcagtca
     6721 gcccgggtgg agctctggga aatcgatctg acagaggtcg gtggtgagcg ttattttttc
     6781 tgtaatgagc agaacgaaaa aggtgagccg gtcacctggc aggggcggca gtatcaggca
     6841 taccccattc agggggcggg gtttgaactg aacggcaggg gctgtgctgc ccgtccgaca
     6901 ctgacggtct ctaacctgca cggcatggtc accgggatgg cggaagatct gcagagtctg
     6961 gtcggcggaa cggtggtccg gcgtaaggtt tacgcccgtt ttctggatgc ggtgaacttc
     7021 gtcaacggaa acagcgacgc cgatccggaa caggaggtga tcagccgctg gcgcatcgag
     7081 cagtgcagcg aactgagcgc ggtcagtgcc tcttttgtgc tggccacacc aacggagacg
     7141 gatggcgcgg ttttcccggg gcgtatcatg ctggcgaata cctgtatgtg gacttaccgt
     7201 tctgatgagt gtggttacac gggcagggca gtggctgacg agttcgacaa accaacgacg
     7261 gatatccgga aggacaaatg cagcaagtgt atgcgcgggt gtgagttgcg caacaatacc
     7321 ggtaatttcg gcggtttcct ttccatcaat aaactttctc agtaaatcca tgacacagac
     7381 agaatcagcg attctggcgc acgcccggcg atgtgcgcca gcggagtcgt gtggattcgt
     7441 ggtgagaacg ccggagggag acaggtatct tcccagcgag aatatctccg gtgagccgga
     7501 ggaacggttc cggatggctc cggaggactg gctgcgggca caaatgcagg gtgagattgt
     7561 ggcactggtc cacagtcatc ccggtggtct gccctggctg agtgaggctg accggcggct
     7621 gcaggtgcag agtgatttgc cgtggtggct ggtctgccgg ggggcgattc acaagttccg
     7681 ctgtgtgccg catctcaccg ggcggcgctt tgagcacggg gtgacggact gttacacgct
     7741 gttccgggat gcttatcatc tggcggggat tgagatgccg gattttcacc gcggggatga
     7801 ctggtggcgt cacggtcaga atctctagct ggataatctg gaggccacag ggctgtatca
     7861 ggtgccgttg tcatcagcac aaccgggcga tgtgctgctg tgctgttttg gttcatcggt
     7921 gccgaatcat gccgccattt actgcggcga tggtgagctg ctgcaccata ttcctgaaca
     7981 actgagtaaa cgagagaggt ataccgacaa atggcagcga cgcacacact ccctctggcg
     8041 tcaccgggaa tggcacgcat ctgcctttac ggggatttgc aacgatttgg ccgccgcatc
     8101 gaccttcgtg tgaaaacggg ggctgaagcc atccgcgcac tggccacaca gctcccggcg
     8161 tttcgtcaga aactgagtga cggctggtat caggtgcgca ttgccgggcg tgatgcaggt
     8221 gaaaccgaat tgtctgcccg tcttaatgag ccgctggcaa atggtgcagt gatccacata
     8281 gtaccgcgtc tggtgggagc taaaagtggc ggtgtgtttc aggcggtgct gggggcagct
     8341 gttatggcgg ttgctatatg gatgccgggg taggaattat ggcgagtaat ctgctgtttt
     8401 ctctcggtgc cagtatgacg cttggcggtg ttgcacagat gctggcaccg aaagccagaa
     8461 ctccccgtac acagacaacg gataacggca aacagaacac ctgtttctcc tcactggata
     8521 acatggttgc ccagggcaat gttctgcctg ttctgtacgg tgaaatgcgc gtggggtcgc
     8581 gggtggtatc tcaggagatc agcacggcag acgaagggga tggtggtcag gttgtggtga
     8641 ttggtcgctg atgcaaaatg tttcatgtga aaccgcctgc gggcggtttt gtcgtttatg
     8701 gagcgtgagg aatgggtaaa ggcagcagta aggggcatac cccgcgcgaa gcgaaggaca
     8761 acctgaaatc cacgcagttg ctgagtgtga ttgatgccat cagcgaaggg ccggttgacg
     8821 gtccggtgga tggattaaaa agcgtgctgc tgaacggtac gccggtcctg gacagcgagg
     8881 ggaagacaaa cttttccggt gttacggtgg tgttccgcgc cggcgagcag gagcagacac
     8941 cgccggaggg gtttgaatct tccggctcag agactgtgct gggtacggaa gtgaaatacg
     9001 acacgccgat cacccggacc atcacgtcgg caaacattga tcgtctgcgc ctgaccttcg
     9061 gtgtgcaggc actggtggaa accacctcaa agggggaccg gaatccgtcg gaagtccgcc
     9121 tgctggttca gatacagcgt aacggtggct gggtgacgga aaaagacatc accattaagg
     9181 gcaaaaccac ttcgcagtat ctggcctcgg tggtggtggg taacctgccg ccgcgcccgt
     9241 ttaatatccg gatgcgtagg atgacaccgg acagcaccac agaccagctg cagaacaaaa
     9301 cgctctggtc gtcgtacacc gaaatcatcg atgtgaaaca gtgctacccg aacacggcgc
     9361 tggtcggcgt gcaggtggat tcagagcagt tcggtaacca gcaggtgagt cgcaattatc
     9421 atcttcgcgg gcgcattctg caggtgccgt cgaactataa cccgcagacg cggcaataca
     9481 gcggtatctg ggacggaacg cttaagccag catacagcaa caacatggcc tggtgtctgt
     9541 gggacatgct cactcatccg agctaaggca tggggaaacg tcgtggtgcg gcagatgtgg
     9601 acaaatgggc gctgtatgtc atcggccagt actgcgacca gtcagtgccg gacggatttg
     9661 gcggcacgga gccgcgcatc acctgtaacg cttacctgac cacacagcgt aaggcgtggg
     9721 atgttctcag tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac gggcagacgc
     9781 tgacgttcgt gcaggaccga ccgtcggata aagtgtggac ctataaccgc agtaatgtgg
     9841 tgatgccgga tgatggcgcg ccgttccgct acagcttcag cgccctgaag gaccgccata
     9901 atgtcgttga ggtgaactgg attgatccgg acaacggtca tgagacggcg acagagcttg
     9961 tggaggacac gcaggccatt gtccgttacg gtcgtaacgt cacgaagatg gatgcctttg
    10021 gctgtaccag ccggggacag gcgcaccgcg ccgggctgtg gctgattaaa acggaactgc
    10081 tggaaacgca gaccgtggac ttcagcgtcg gcgcagaagg gcttcgccat gtaccgggtg
    10141 atgtcattga aatctgcgat gatgattatg ccggtatcag caccggtggt cgcgtactgg
    10201 cggtgaacag ccagacccgg acgctgacgc tcgaccgtga aatcacgctg ccgtcctccg
    10261 gtaccacgct gataagcctg gttgacggaa atggcaatcc ggtcagcgtg gaggttcagt
    10321 ccgtcaccga cggcgtgaag gtgaaagtga gccgggttcc tgacggcgtt gcagaataca
    10381 gcgtgtgggg gctgaagctg ccgacgctgc gccagcgcct gttccgctgc gtgagtatcc
    10441 gtgagaacga cgacggcacg tatgccatca ccgccgtgca gcatgtgccg gaaaaagagg
    10501 ccatcgtgga taacggggca cactttgacg gtgaccggcg cggcacggtg aatggtgtca
    10561 cgccgccagc ggtgcagcat ctgaccgccg aagtcaccgc agacagcggg gaatatcagg
    10621 tgctggagcg atgggacacg ccgaaggtgg tgaagggtgt gagcttcctg cttcgcctga
    10681 ccgtggcagc ggacgacggc agtgagcggc tggtcagcac agccaggacg acggaaacca
    10741 cataccgctt caggcagctg gcgctgggga attacagtct gacagtccgg gcggtaaatg
    10801 cccgggggca gcagggcgat ccggcgtcgg tatcgttccg gattgcggca ccggcagcgc
    10861 ctgtcactat tgaactgata ccggggtatt ttcagataac ggtggtcccg aaactggctg
    10921 tatatgaccc gacggtgcag tttgagttct ggttttcgga aaagcggatt gcggatatca
    10981 ggcaggttga aaccagcgcc cgctatcttg gcacggcgct gtactggata gccgccagta
    11041 tcaatatcag gccgggccat gattattatt tttacgttcg cagtgtgaac accgttggca
    11101 aatcggcatt tgtggaggcc gtcggtcggg cgagcgatta tgcggaaggt tatctgagtt
    11161 tttataaagg gttgatcaat aaaacgcatc tcggcaagga gttgtggacg cagattgata
    11221 acggtcagct tgcgccggac ctgactgaaa tcaggacgtc cattacgaat gtcagcaatg
    11281 aaatcacgca aaccgtcaat aaaacactgg aaaatcagag tgctgcaatc cagcagatac
    11341 agaaagttca ggttgataca aataataacc tgaacagcat gtgggctgtg aagctgcaac
    11401 agatgaagga cggacgcctt tatattgcgg gtatcggtgc cggtattgag aatacgccag
    11461 caggaatgca gagtcaggtg ctgctggcgg cagacaggat tgcgatgatt aatcctgcga
    11521 atggcaacac aaagccgatg tttgttggtc agagcgatca gatattcatg aacgacgtgt
    11581 tcctgaaacg cctgacggct ccgaccatta ccagcggcgg taatcctccg gcattttccc
    11641 tgacacctgg cggacggctg acggcgaaaa atgccgatat cagcggtaac gtgaacgcga
    11701 actccgggac gctcaacaac gtcacgatta ataagaactg tcgggttctg ggaaaattgt
    11761 ccgcgaacca gattgaaggc gatctcgtta aaacagtggg caaacctttc ccacgggact
    11821 cccgggcacc ggagaggtgg ccatcaggga ccattaccgt cagggtttat gacgatcagc
    11881 cgtttgatcg gcaaattgtt attcccgcgg tggcgtttcg cggtgctaaa catgagcgga
    11941 agaataacaa tatttattcg tcatgccgcc tgatagtgaa gaaaaacggt gctgaaattt
    12001 ataaccgaac gaccctggat aatacgctga tatatacggg tgttattgat atgcctgccg
    12061 gtcacggtca catgacgctg gagttttctg tatcggcatg gctggtaaat ggctggtatc
    12121 ccacagcaag tatcagcgat ttgctggttg ttgtgatgaa gaaagccact gcaggcatca
    12181 cgattagctg aattttataa cccagatacg ggcaccagaa atggtgcctt ttttattgca
    12241 gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca ttttgtccgc agccatctgt
    12301 ctggctgtat ccggtgcgcc tgcatgggcg tctgaacatc agtccacgct gagcgccggg
    12361 tatcttcagc cccatactga tatgccaggc agcgatgacc tgaagggcat taacgtgaaa
    12421 taccgttatg agtttacgga cacgctgggg ctggtgacgt cattcagcta tgccaacgct
    12481 gaagatgagc aaaaaacgca ttacagcgat acccgctggc atgaggattc cgtgcgtaac
    12541 cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga atgaatggtt cagcgcgtat
    12601 gcgatggcgg gcgtggctta cagccgtgtg tcgaccttct ccggggatta tctccgcgta
    12661 actgataaca aggggaaaac gcacgatgtg ctgaccggga gtgatgacaa ccgccacagc
    12721 aacacgtctc tggcgtgggg ggctggcgtg cagtttaacc cgaccgaatc cgtggccatt
    12781 gaccttgctt atgaaggttc cggcagtggc gactggcgca ctgacggttt cattgtgggt
    12841 gtcggttata agttctgatt agccaggtaa cacagtgtta tgacagcccg ctggttcagg
    12901 cgggcttttt gtggggtgaa tatggcagta aagatttcag gtgtactgaa agatggggcg
    12961 ggtaaacctg tcgtaaattg tgcgattgaa ctgcgggcca gaagaaccag tccgaccgtt
    13021 gtggcacacg ttgttgccac ttgcgtgacg gacaataacg gtgcttatgt gattgaggct
    13081 gagccggggt attacgaggt tgcgcttcac tgtaacggct ggcagccaac ccgtgtcggg
    13141 gatattgatg tggcaccgac tgatgcaccg gggacactga acgcgtttct gaatgcacca
    13201 aaggatggtg atttacgtcc ggaggtgatg aagcgctttg aggaaatggt ggcgcaggcg
    13261 cagcagagtg ccggggc
//
LOCUS       DADMEQ010000108        13265 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.109, whole genome shotgun sequence.
ACCESSION   DADMEQ010000108 DADMEQ010000000
VERSION     DADMEQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.109"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..184)
                     /locus_tag="JNZ05_003123"
     CDS             complement(<1..184)
                     /locus_tag="JNZ05_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566257.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAA"
     gene            503..1084
                     /gene="mdaB"
                     /locus_tag="JNZ05_003124"
     CDS             503..1084
                     /gene="mdaB"
                     /locus_tag="JNZ05_003124"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HAY8566258.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTLK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            1115..1429
                     /locus_tag="JNZ05_003125"
     CDS             1115..1429
                     /locus_tag="JNZ05_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY8566259.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSKAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1477..3369)
                     /gene="parE"
                     /locus_tag="JNZ05_003126"
     CDS             complement(1477..3369)
                     /gene="parE"
                     /locus_tag="JNZ05_003126"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAY8566260.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGAVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3398..3979)
                     /gene="yqiA"
                     /locus_tag="JNZ05_003127"
     CDS             complement(3398..3979)
                     /gene="yqiA"
                     /locus_tag="JNZ05_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HAY8566261.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTRQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3979..4806)
                     /gene="cpdA"
                     /locus_tag="JNZ05_003128"
     CDS             complement(3979..4806)
                     /gene="cpdA"
                     /locus_tag="JNZ05_003128"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HAY8566262.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4831..5253)
                     /locus_tag="JNZ05_003129"
     CDS             complement(4831..5253)
                     /locus_tag="JNZ05_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HAY8566263.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTMVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5254..5883)
                     /gene="nudF"
                     /locus_tag="JNZ05_003130"
     CDS             complement(5254..5883)
                     /gene="nudF"
                     /locus_tag="JNZ05_003130"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HAY8566264.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6088..7569
                     /gene="tolC"
                     /locus_tag="JNZ05_003131"
     CDS             6088..7569
                     /gene="tolC"
                     /locus_tag="JNZ05_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HAY8566265.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7717..8388
                     /locus_tag="JNZ05_003132"
     CDS             7717..8388
                     /locus_tag="JNZ05_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HAY8566266.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8394..9554
                     /locus_tag="JNZ05_003133"
     CDS             8394..9554
                     /locus_tag="JNZ05_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY8566267.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQCGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9592..10407)
                     /gene="ygiD"
                     /locus_tag="JNZ05_003134"
     CDS             complement(9592..10407)
                     /gene="ygiD"
                     /locus_tag="JNZ05_003134"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HAY8566268.1"
                     /translation="MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMT
                     LPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLV
                     ELLAPIPVTLDKEAWGFDHGSWGVLIKMYPDADIPMVQLGIDSSKPAAWHFEMGRKLA
                     ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVN
                     YLDHEGGALSNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10523..11296
                     /gene="zupT"
                     /locus_tag="JNZ05_003135"
     CDS             10523..11296
                     /gene="zupT"
                     /locus_tag="JNZ05_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HAY8566269.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11354..11524)
                     /locus_tag="JNZ05_003136"
     CDS             complement(11354..11524)
                     /locus_tag="JNZ05_003136"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566270.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11785..12438)
                     /gene="ribB"
                     /locus_tag="JNZ05_003137"
     CDS             complement(11785..12438)
                     /gene="ribB"
                     /locus_tag="JNZ05_003137"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HAY8566271.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12543..12691)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12753..13103
                     /gene="ubiK"
                     /locus_tag="JNZ05_003138"
     CDS             12753..13103
                     /gene="ubiK"
                     /locus_tag="JNZ05_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HAY8566272.1"
                     /translation="MTYPYRTTMVLNTYQYRETTMIDPKKIEQIARQVHESMPKGIRE
                     FGEDVEKKIRQTLQAQLTRLDLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTE
                     IKKQPDPETLPPTL"
BASE COUNT         3386 a         3525 c         3195 g         3159 t
ORIGIN      
        1 atgctgcaat cattgcttgc aatatacgat cttcgcctga aacggaaggt atactgcaaa
       61 tattgcctcc cgactcaagc atccagtcat acttattgaa agcaaaaaaa gaatctatta
      121 accaataagt aagcattata tatagcaatg cttcaaatat tgttttaatt ttctgcttta
      181 acatatatca gtacgctcat aaaaatccat ttataattat caacatgaca cgttgatatt
      241 ttcatctttt acttattcaa agccatattt tcaatcccca aaaaaaccga accaaacgtg
      301 taaattattc cggtacgagt aagaattatg aggaatggct atcagtattg tcattttcag
      361 aaaatattta tcctgcatcg gtgagtcaga gctagatcag acttttgcta aattcgcaaa
      421 agactttgca cattttgcta atttcaccgt accgctctgt gacgtactat agtcgacaaa
      481 cgtctcacct tgaggttaaa aaatgagcaa catcctgatt atcaacggcg cgaaaaaatt
      541 cgcccactcc aatggtcaac tgaacgacac cctgaccgaa gtcgcggatg gcacactgcg
      601 cgacctcggg catgatgtcc gcatcgttcg cgccgacagc gactacaatg tcaaagcgga
      661 agtacaaaac tttctctggg ctgatgtggt gatctggcag atgccaggct ggtggatggg
      721 cgcgccgtgg acagtgaaaa aatatattga tgatgtattc accgaaggtc acgggactct
      781 gtatgccagc gatggtcgta ccctcaaaga tccgtcgaaa aaatacggtt ccggcggcct
      841 ggtacagggc aaaaaatata tgctttctct aacctggaac gcaccaatgg aagccttcac
      901 cgaaaaagat cagttcttcc acggcgttgg cgttgacggt gtgtatctgc cgttccataa
      961 agcaaaccaa ttcctcggta tggaaccgct gccgacattt atcgctaatg acgtgataaa
     1021 aatgcctgat gttccccgct atactgaaga atatcgcaag catcttgtgg aaatttttgg
     1081 ttaactagag ctcaggcttt agaaggagtt aaccatgctt accgtaatcg cagaaatccg
     1141 tactcgtcct ggtcaacatc accgtcaggc ggtattggat cagtttgcta aaatcgttcc
     1201 aaccgtactg aaagaagaag gttgccacgg ctatgcgcca atggtggatt gcgctgctgg
     1261 cgtgagtttc cagtctatgg caccggattc tatcgtgatg attgagcagt gggaaagcat
     1321 cgcgcacctt gaagcgcatc tgcaaacccc gcacatgaag gcgtacagca aagccgtaaa
     1381 aggcgacgtg ctggagatga atatccgtat tctgcagcca gggatttaat cctgccttgt
     1441 ttgcccggcc atcctgaccg ggcaatgttc tttcctttaa acctcaatct ccgccatgtc
     1501 gcctttctct tgcaaccagt tgcggcgatc ttccgagcgt ttcttcgcca gcagcatatc
     1561 catcatcgcg tcagtacgct gatcgtcttc atcatcgata gtcaactgca ccagacggcg
     1621 agtgttcgga tcaagcgtgg tttcacgcaa ttgcatcggg ttcatttccc ccagaccttt
     1681 aaaacgctgg acgttcggct tgcctttctt gcgttttaat tgctcaagta cgccctcttt
     1741 ctcttcttcc gtcagcgcgt aataaacctc tttcccgaga tcaatacggt agagcggtgg
     1801 cagtgcgacg taaacgtgac cgtgtttcac caacgcgcgg aagtgtttta cgaacaaagc
     1861 gcagagcagc gtggcaatgt gcagaccatc agagtccgca tccgcgagga tacagatttt
     1921 gccataacga agctggctca gatcgtcgct gtcaggatcg ataccgatcg ctaccgaaat
     1981 atcatgcact tcctgcgagg ccagcacttc gtcggaagaa acttcccagg tgttaaggat
     2041 cttacctttc agcggcatga tcgcctgata ttcacgatcg cgcgcctgct tggcagatcc
     2101 gcctgcggag tcaccttcca cgaggaacag ttcggtacgg ttaaggtcct gcgcggtaca
     2161 atcagccagt ttgccaggca gcgccgggcc gctggtcagc tttttacgca ccactttttt
     2221 agccgcacgc atacgacgct gggcgctgga aatcgccatc tctgccagca actccgccgc
     2281 ctgaacgttc tggttcagcc acaggatgaa ggcatctttc accgcgcccg aaacgaatgc
     2341 cgcacactga cgcgaagaga gacgctcttt ggtctgcccg gcaaactgcg gatcctgcat
     2401 tttaaccgac agaacatagg cgcagcgatc ccagatatct tccgccgaca gcttcacgcc
     2461 gcgcggcaga atgttgcggt attcacagaa ctcacgcatc gcgtccaaca ggccctgacg
     2521 cagaccatta acatgggtac cgccctgcat cgttgggata aggttgacgt agctttcggt
     2581 cagcagttca ccgccttccg gcagccacag tagcgcccag tccacagctt cagtatcgcc
     2641 agcgaaatta ccgataaacg gtttttctgg cagcgtcggt aaaccgttta ccgcttccgc
     2701 caggtaatca ttcagaccgt cctgatagca ccagcgctgt tcggtgttgt tgatctcatc
     2761 tttaaaggta atctcaacgc cggggcacag tactgctttg gccttcagca catgcgtcag
     2821 gcgtgaaaca gaaaaacgcg ggctgtcaaa gaaggtttca tccggccaga agtgcacgct
     2881 ggtaccggta ttgcgtttac cgcaagtgcc gacaacctgt aaatcctgca ccttttcgcc
     2941 attttcaaag gcgatgttat aaacctgacc atcgcggcgc acgttaactt ctacgcgctt
     3001 cgacaaggcg ttaactaccg aaatccccac gccatgcagg ccgccagaga actggtaatt
     3061 tttgttagag aatttaccgc ctgcatgcag acggcaaaga atcagttcaa ccgccggtac
     3121 tccttcttcc gggtgaatat ccaccggcat cccgcgccca tcgtcaatca cttctaacga
     3181 ctggtcagca tgtaagatta catccacgcg ttttgcgtga cccgccagtg cttcatcgac
     3241 actgttatca ataacttctt gccccaaatg gttagggcga gtggtatcgg tatacatccc
     3301 cggacggcgg cgaaccggct caagcccggt gagtacctca atggcatcag cgttataagt
     3361 ttgcgtcatg gtttaagtta gcaattcgag ttgatcgtca gagatggtgc agaccaagaa
     3421 aatcgacgat cgggttgaaa taatcttcga agcccgtgaa tgcgtggttg ccgccttcta
     3481 tgacagtctg gcggcaggaa gcgtagtacg ccaccgcctg gcggtaatcc agcacttcat
     3541 ctcccgtctg ttgcagcagc cagatcaaat ccggcgcttc cagcgggtca atctgcatga
     3601 ctttgagatc gtaaatatgg cgtgactcta gcacatattg ctgcctggtg taggggttct
     3661 cgttctgacc gagatagtcc gtcagcaatt caaacgggcg taccgccggg tttaccacca
     3721 ctgcgggcag cataaaacat tgcgacaacc aggtggcgta atatcccccc agtgacgaac
     3781 cgacaatacc cagcgaatcg ccgccatgtt ccaggacaat ggattccagc agttctgccg
     3841 cgtcggaagg atacggcggc aactgcggaa tgatcatctc aacgtcaggg tgatgttccg
     3901 ccagccagtt ttttaacaag ctcgcttttg cagagcgcgg cgagctgttg aaaccgtgta
     3961 aataaagaag cgtagacatc agtagccttc tgaagcggta tcaggttgga aacgtgtgtc
     4021 cgccaggcga tgcacctcgg tggtcagcgt gccatcagca tgtaactcga gagtacgcca
     4081 gccgggcgcg atagtatcca gcgtaaagtt ggaacagtgc ggcttaaact gcacacaggt
     4141 cgacggcgtt gccagcaggc ggcgaccgtt ccagtcgaga tccagctcct gatgaatatg
     4201 accgcacagc aagtatttga cgtgcggaaa cttcgccagc acggtatcca gttcgcccgc
     4261 gttacgcaga ctgtgttgat cgagccaact acaacccgca ggtagcggat gatgatgcag
     4321 cagcagcaac gtatggcgtt ctggcgcatc ggccagttta cgttccagcc actcaagctg
     4381 aaactcgctc agctcaccgt gcggcacgcc aaacacctgg ctatccagca acaggatttg
     4441 ccattgctca ccaataaaca cccgcttcgc cggggagata cccgcatcct gtaacgcgct
     4501 gtacatcgcg ggctggaaat cgtggttgcc cggcagccag acgcagggcg cacgaaaact
     4561 tgcgatgcct tcagcgaaat gctgataggc cgcagaggat tgatcctgcg ctaaatcacc
     4621 tgtcgcgaca atcaggtcga attcgtgctg gtgtggacga atcgcctcca gcaccgcctg
     4681 gtagctttcc caggtgttta cccctaacag ggcttcgtgc ttttgtgcaa acaggtgagt
     4741 atcggtaatt tgtaaaatcc ttcctctggc ctcaccagcc agaggaaggg ttaacaggct
     4801 ttccaaatgg tgtccttagg tttcacgacg ctagtaaacc ggaatcgcca tcgctccatg
     4861 tgctaaacag tatcgcaacc agtccgctaa aaactggtta atttgatgct tttcgtcgcg
     4921 ttgatgcaac tttttattag gataatcata ccgcgctttg aagcgaaaaa tctgctggct
     4981 tgaacacact tcagccacca tcgcgtcatg atacagacgc accgtcattg acggaaggct
     5041 ccagtaactg atcgcgggcg cagtctgttc tattgtcacc atggtagtgt atcgggtcga
     5101 ttccacaatc gtcagccgat attgtgcgtt tgccacctga tagcttacag tttcgccggg
     5161 tgcgtcattg cgcggtaaca aacggcgcaa ttgtgaaaag ttcatctcgc acaggcgcat
     5221 catttcagga aagtcaggtg tgtaacgctt catttatgcc cactcatttt ttaacgcttg
     5281 atgatgcagc tgcagccatt gcaaagcgat gaccgacgct gcgttgtcga ttttcccctc
     5341 ttctacccac tggtatgcct gttcccggct taccacatga acgcgaatat cttcgttttc
     5401 atcagccaga ccatgaatac cgcttgcggt cgtggcgtcc acttcgccca ccataattga
     5461 cgaacgctca ctggtgcccc ccgggcttgc caggaaactt aacaccggtt tggtccgttt
     5521 gactatcagt cccgcctctt caatcgcttc gcgacgggca acatcttcca cactttcacc
     5581 ctcttcaatc atcccggcaa ccatctccag tagccagggg gtttcgctgg tgtcgtacgc
     5641 ggcaatccga atctgctcaa tcagcacaac ttcatcacgc actgggtcaa agggtagcaa
     5701 gactgcggcg tgaccgcgct caaaaatttc ccgccgtacc tcatgactca tttgcccgtt
     5761 gaatagacga tgacgaaatc tataaagatc taatgaaaaa aagccgcgat aaagtgtttc
     5821 tcgtgcaata atttctacat cgtttttgcc aaatgtaacg ggcaggttgt ctggcttaag
     5881 cattgttaat gtcctggcac taatagtgaa ttaaatgtga atttcagcga cgtttgactg
     5941 ccgtttgagc agtcatatgt taaattgagg cacattaacg ccctatggca cgtaacgcca
     6001 accttttgcg gtagcggctt ctgctagaat ccgcaataat tttacagttt gatcgcgcta
     6061 aatactgctt caccacaagg aatgcaaatg aagaaattgc tccccattct tatcggcctg
     6121 agcctttctg ggttcagttc gttgagccag gccgagaacc tgatgcaagt ttatcagcaa
     6181 gcacgcctta gtaacccgga attgcgtaag tctgccgccg atcgtgatgc tgcctttgaa
     6241 aaaattaatg aagcgcgcag tccattactg ccacagctag gtttaggtgc agattacacc
     6301 tatagcaacg gctaccgcga cgcgaacggc atcaactcga acgcgaccag tgcgtccctg
     6361 cagttaactc aatccatttt tgatatgtcg aaatggcgtg cgttaacgct gcaggaaaaa
     6421 gcagcaggga ttcaggacgt cacatatcag accgatcagc aaaccttgat cctcaacacc
     6481 gcgaccgctt atttcaacgt gttgaatgct attgacgttc tttcctatac acaggcacaa
     6541 aaagaagcga tctaccgtca attagatcaa accacccaac gttttaacgt gggcctggta
     6601 gcgatcaccg acgtgcagaa cgcccgcgcg cagtacgata ccgtgctggc gaacgaagtg
     6661 accgcacgta ataaccttga taacgcggta gagcagctgc gccagatcac cggtaactac
     6721 tatccggaac tggcggcgct gaatgtcgaa aactttaaaa ccgacaaacc acagccggtt
     6781 aacgcgctgc tgaaagaagc cgaaaaacgc aacctgtcgc tgttacaggc acgcttgagc
     6841 caggacctgg cgcgcgagca aattcgccag gcgcaggatg gtcacttacc gactctggat
     6901 ttaacggctt ctaccgggat ttctgacacc tcttatagcg gttcgaaaac ccgtggtgcc
     6961 gctggtaccc agtatgacga tagcaatatg ggccagaaca aagttggcct gagcttctcg
     7021 ctgccgattt atcagggcgg aatggttaac tcgcaggtga aacaggcaca gtacaacttt
     7081 gttggtgcca gtgagcaact ggaaagcgca catcgtagcg tcgtgcaaac cgtacgttcc
     7141 tccttcaaca acattaatgc ttctatcagt agtattaacg cctacaaaca agccgtagtt
     7201 tccgcgcaaa gctcattaga cgcgatggaa gcgggctact cggtcggtac gcgtaccatt
     7261 gttgatgtgt tggatgcgac caccacgctg tacaacgcca agcaagagct ggcgaatgcg
     7321 cgttataact acctgattaa tcagctgaat attaagtcag ccctgggtac gttgaacgag
     7381 caggatttgc tggcactgaa caatgcgctg agcaaaccgg tttccactaa tccggaaaac
     7441 gttgccccgc aaacgccgga acagaatgct attgctgatg gttatgcgcc tgatagcccg
     7501 gcacccgtcg ttcagcaaac atccgcacgc actaccacca gtaacggtca taaccctttc
     7561 cgtaactgat gacgacgacg gggcttcggc cccgtctgaa cgtaaggcaa cgtaaagata
     7621 cgggttatct gccgcattct tcccccttct cgcttcaatt tcgaccagcc atcctctatt
     7681 ctgatgggta tttaccactg gtcccggaag acaaaaatga aacggacaaa atccatacgc
     7741 cacgcatcgt tccgcaaaaa ctggagcgca cgccatctga caccagtcgc tctcgcggtt
     7801 gccactgttt ttatgctggc tggctgtgaa aagagtgatg aaacagtgtc tctctatcaa
     7861 aatgctgacg actgttcagc tgcaaaccca ggcaaaagcg ccgaatgtac caccgcgtac
     7921 aacaatgcgc tgaaagaagc cgaacgtact gcgccgaaat acgccacccg tgaagactgt
     7981 gttgctgaat ttggtgaagg tcagtgtcag caagcaccag cccaggctgg catggcacca
     8041 gaaaaccagg cgcaggctca gcaatccagc gggagtttct ggatgccgct gatggccggt
     8101 tacatgatgg ggcgtctgat gggcggcggc gcgggatttg cacagcagcc gctgttctcc
     8161 tcgaaaaacc cggccagtcc ggcttacggt aaatataccg acgcgacggg taaaaactat
     8221 ggcgcagccc agccaggccg caccatgacc gtaccgaaga cggcaatggc accaaaaccg
     8281 gcgaccacca ctaccgttac ccgtggcggt tttggtgaat ctgttgccaa acaaagcact
     8341 atgcagcgta gcgcaaccgg cacctcttct cgttcaatgg gtggctgata ccgatggaaa
     8401 gagtcagtat taccgagcgc ccggactggc gtgagaaagc ccacgaatac ggtttcaatt
     8461 ttcacaccat gtacggcgag ccgtactggt gtgaagatgc ttactacaag ttgaccctcg
     8521 cccaggttga aaagctggaa gaagtcaccg ccgaactgca ccagatgtgc ctgaaagtgg
     8581 tggaaaaagt gatcgccagc gatgagctga tgaccaaatt ccgcatacca aaacacacct
     8641 ggagttttgt gcgtcaatca tggctgactc accagccatc gctttattcg cgtcttgatc
     8701 tggcgtggga tggcactggc gaacctaaac ttctggaaaa taacgccgat acgccaacgt
     8761 cactatacga ggcggcgttc tttcagtgga tctggctgga agatcagctt aacgccggta
     8821 acttgccgga gggcagcgac cagtttaaca gtctgcaaga aaaactgatc gatcgcttcg
     8881 ttgagctgcg tgaacagtgt ggcttccagt tgctgcatct cacctgctgt cgcgacacgg
     8941 tggaagatcg cggaaccatt cagtatttgc aggactgcgc aaccgaagct gaaatcgcca
     9001 ccgagttcct ctacatcgac gagataggtt taggcgaaaa aggccagttc acggatttac
     9061 aggatcaggt gatttctaac ctgttcaagc tttatccgtg ggaatttatg ctgcgcgaaa
     9121 tgttctccac caagctggaa gatgctggcg tacgctggct ggaaccggcg tggaagagta
     9181 ttatctccaa caaggcactt ctaccgctac tgtgggagat gttcccgaat cacccgaacc
     9241 tgctgcccgc ttattttgcg gaagatgatc atccgcaaat ggaaaaatat gtggttaaac
     9301 cgatcttctc ccgtgaaggc gcaaacgtgt cgatcattga gaacggcaaa accattgaag
     9361 cagcggaagg tccgtatggc gaagaaggga tgattgttca gcaattccac ccgttaccga
     9421 aattcggcga cagctatatg ctgattggta gctggctggt gaacgatcaa cctgccggaa
     9481 ttggcattcg tgaagaccgt gcgttgatca cccaggatat gtctcggttt tatccacata
     9541 tttttgttga ataagccacg ataccggatg gcactcgcca tccggtaatt gttagcctat
     9601 ctgcaccgac agcatactta aactgcccat ttctatacca tcgaccggaa tggtaattgg
     9661 ctcctgccca tcccacacac ctaacacata caacaacggc agatagtgct ctggcgttgg
     9721 gttcgataac gcgccacctt catggtcgag ataattcacc agaggatgct gctctaccgg
     9781 cccttgccac gtcagattcg ctttcacata ctcattaaac gacgtcgccc acggatacgg
     9841 tgaactatca ccgtgccact tcactgtgcg caggttatgc accacgttac cgctggcgac
     9901 caacattatt ccttcatcac gcaacgccgc gagtttgcgc cccatttcga aatgccaggc
     9961 ggcaggtttg ctactgtcga tacccaactg caccataggg atatcggcgt caggatacat
    10021 cttaatcagc acgccccacg agccgtggtc aaagccccag gcttctttat ccagcgtcac
    10081 cgggatcggc gctaacagct caaccagacg ctgtgccagc gcaggcgaac ccggagcagg
    10141 ataatgcgta tcgtacagcg cctgcgggaa gccaccaaag tcatgaatcg tgggcggcgt
    10201 ctccatcgcg gtcactcctg ttccacgggt aaaccagtga gccgaaacca ccacaatcgc
    10261 ttgcgggcgt ggcaatgtca tccccaactt ctgccagctg cgggtataca aattatcttc
    10321 cagcacgttc atcggactac cgtgacctaa aaacaatgct ggcatacgtg ttgaagacat
    10381 gatgatatcc ttaactaaag gtgtcatttt gatatcctca caatacgctt gttcggcgga
    10441 gtaagaaccc ggataacaat gatgatgatc atcagttatt ttgacgatct gcctgaaggt
    10501 gaagatttat aaggagttgt cgatgtcagt acctctcatt ctgaccatac tggcgggggc
    10561 agccacgttt attggcgcgt ttctcggcgt tctcggacaa aaaccctcga accgcttact
    10621 ggcgttttcg ctaggttttg cggcggggat catgttgctc atctcattaa tggaaatgct
    10681 tcctgccgca ctagcagctg aaggaatgtc gccggtgttg ggttatggaa tgtttatctt
    10741 cggtctgctt ggctactttg gcctggaccg catgttgccg catgcgcatc cgcaggattt
    10801 aatgcaaaaa tcggtgcagc tgttgccaaa atcgatcaag cgcacagcca ttctgctcac
    10861 tctcggcatc agtctgcata acttcccgga agggattgcc acctttgtca cggcgagcag
    10921 caacctggag ctgggatttg gcatcgcact ggccgtcgcg ttgcacaata tccctgaagg
    10981 tctggcagtg gcaggcccgg tttatgcggc aacggggtct aaacgcaccg cgattctgtg
    11041 ggcggggatt tctggactag cagaaattct tggtggtgtg ctggcgtggt taatcctcgg
    11101 tagcatgatt tccccggtgg tcatggcggc aatcatggcg gcggttgcag gaattatggt
    11161 ggcgctctcg gttgatgaat taatgccgct cgccaaagag attgacccta ataataaccc
    11221 cagctatggc gtactatgtg gaatgtcagt gatgggattc agtttagtgc tgctacaaac
    11281 ggcgggaatt ggttaaaaac aacatcagaa gttgtccagc acatggacaa cttctgccag
    11341 cttcacgaaa gccttattct accttttcgg ctgtctcttc tctcgtactg tttaagtatt
    11401 tgttccgcgc ttcaagcagt gcgtctctgt cctggcgaaa cgcccgacaa taacgtttca
    11461 aatgcaggaa ataacccacc acaaccagag caatcaatac aatccagtac caggcgataa
    11521 acatcctttt acccttaata tttaacaata ctaaacattt agcgcataaa tttcacatat
    11581 cctttttcgg atatattcat atggtcgcat aataacggaa caattatatg attttgatca
    11641 taatgaaaaa gaatgcctga tattaatgaa attttctcat agaaagagaa gcggaattaa
    11701 catcttgcat agcagggcaa aataaaaacc gaagccagct acaacttcgg tttcttgatt
    11761 aaggcagtaa ttaagcagcg gttttcagct ggctttacgc tcatgtgcct gacggtatgc
    11821 caccaggtct tcaatagtca cgagcgccat attgtgttta ttggcaaact caatgcactc
    11881 tggtgcacgc gccatcgtgc catcgtcatt agtcagctca cacagcacac cagccggttt
    11941 aaagcctgcc agcgtcatca aatcaatagt cgcttcagta tgaccgccac gcgtcagtac
    12001 accacctgcc tgcgcgcgaa gtgggaaaac gtggcccggg cgattcagat cagaaggttt
    12061 tgcgccatcg gcaatcgctg cgcgaacggt cgtaatacga tcagcggcag aaacaccggt
    12121 agtcacacct tcagccgctt caatggtcac ggtaaaaccg gtgccatagg cgctggtgtt
    12181 attttctacc atcattggca gatcaagttg tttacggcga tcttcagtga tgcacaggca
    12241 aacaataccg ctaccgtggc gaatggtcag cgccatctgc tcaacggtca tggtttctgc
    12301 cgggaagatc atatcgcctt cgttttcacg gtcttcatca tcaagcacca ttacaccgcg
    12361 tccttcacgc agcgcagcca gtgcattttc aacacgttcg aaaggcgtac caaaagagga
    12421 aagtagcgtc tgattcatgg taaaaaaaac ctcactaaaa ttatggttac cagaatcagg
    12481 gcagtcttag gagtggcggc atatagccaa aataacgtga gcgggtccat gcccgacaga
    12541 atcgttactc tctcccatcc ggactctaac cgtcggcccc ggaattacac cggatctgct
    12601 gtcctttgag ttcgcaccca aagcgctcgc gggctttcaa ctgagttgat ttaccgccgg
    12661 tggggaattt cgccccgccc tgagaataag cggattcact ataactctaa tgattagcgg
    12721 cagcaacgca tagcttcaca taattctggt ttatgactta cccttatcgc actacaatgg
    12781 tactcaacac ctatcagtac agggaaacta caatgattga cccgaaaaaa attgagcaaa
    12841 tcgctcgcca ggttcacgaa tcaatgccta aaggaatcag ggagttcggg gaagatgtgg
    12901 agaaaaaaat ccgccaaacc ctacaagctc agctgacgcg cctcgacctt gtaagccgtg
    12961 aagagttcga cgtccaaacg caagtcctgt tacggactcg tgagaaactg gcgctgcttg
    13021 aacaaagaat cagcgaactg gaaaatcgca gtactgagat caaaaagcag ccagatccag
    13081 agactctccc cccaacgctg taaacatttg tctttaatgc actgtaaatt atgaattaat
    13141 cctctaattt acagtgtttt ctattacatt atcgcttaat cccattcaga gtatcatcac
    13201 aaaaatgaat gattgcaatt gaaaatataa ttgttatgta ttctttaagt tttacttaat
    13261 cgata
//
LOCUS       DADMEQ010000109        13222 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.110, whole genome shotgun sequence.
ACCESSION   DADMEQ010000109 DADMEQ010000000
VERSION     DADMEQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.110"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            94..435
                     /gene="yneK"
                     /locus_tag="JNZ05_003139"
                     /pseudo
     CDS             94..435
                     /gene="yneK"
                     /locus_tag="JNZ05_003139"
                     /inference="COORDINATES: protein motif:HMM:NF033404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            465..1205
                     /gene="yneK"
                     /locus_tag="JNZ05_003140"
     CDS             465..1205
                     /gene="yneK"
                     /locus_tag="JNZ05_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAY8566273.1"
                     /translation="MFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQFPMINLGAPI
                     KRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSEVHRWFVNKD
                     PNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQESPHFQDLFL
                     FFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKNETPPKVTHE
                     DLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            1355..2545
                     /gene="ydeA"
                     /locus_tag="JNZ05_003141"
     CDS             1355..2545
                     /gene="ydeA"
                     /locus_tag="JNZ05_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HAY8566274.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2570..3235)
                     /gene="marC"
                     /locus_tag="JNZ05_003142"
     CDS             complement(2570..3235)
                     /gene="marC"
                     /locus_tag="JNZ05_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HAY8566275.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3447..3881
                     /gene="marR"
                     /locus_tag="JNZ05_003143"
     CDS             3447..3881
                     /gene="marR"
                     /locus_tag="JNZ05_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HAY8566276.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3901..4284
                     /gene="marA"
                     /locus_tag="JNZ05_003144"
     CDS             3901..4284
                     /gene="marA"
                     /locus_tag="JNZ05_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HAY8566277.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            4316..4534
                     /gene="marB"
                     /locus_tag="JNZ05_003145"
     CDS             4316..4534
                     /gene="marB"
                     /locus_tag="JNZ05_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HAY8566278.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4565..5464)
                     /gene="eamA"
                     /locus_tag="JNZ05_003146"
     CDS             complement(4565..5464)
                     /gene="eamA"
                     /locus_tag="JNZ05_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HAY8566279.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            5728..6789
                     /gene="ydeE"
                     /locus_tag="JNZ05_003147"
     CDS             5728..6789
                     /gene="ydeE"
                     /locus_tag="JNZ05_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HAY8566280.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAA"
     gene            6973..7068
                     /gene="mgtS"
                     /locus_tag="JNZ05_003148"
     CDS             6973..7068
                     /gene="mgtS"
                     /locus_tag="JNZ05_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HAY8566281.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(7317..7523)
                     /locus_tag="JNZ05_003149"
                     /pseudo
     CDS             complement(7317..7523)
                     /locus_tag="JNZ05_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(7588..9633)
                     /gene="dcp"
                     /locus_tag="JNZ05_003150"
     CDS             complement(7588..9633)
                     /gene="dcp"
                     /locus_tag="JNZ05_003150"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HAY8566282.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            9770..10516
                     /gene="ydfG"
                     /locus_tag="JNZ05_003151"
     CDS             9770..10516
                     /gene="ydfG"
                     /locus_tag="JNZ05_003151"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HAY8566283.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAIWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            10605..11291
                     /locus_tag="JNZ05_003152"
     CDS             10605..11291
                     /locus_tag="JNZ05_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY8566284.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSNWFSEE"
     gene            11469..11672
                     /gene="ydfZ"
                     /locus_tag="JNZ05_003153"
     CDS             11469..11672
                     /gene="ydfZ"
                     /locus_tag="JNZ05_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HAY8566285.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(11708..13168)
                     /locus_tag="JNZ05_003154"
     CDS             complement(11708..13168)
                     /locus_tag="JNZ05_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY8566286.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVAEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         3404 a         3302 c         3064 g         3452 t
ORIGIN      
        1 acagcgccac gcatcacctg cctttgatag ttcttgcagc tcaaatgccc tatatagtga
       61 tgttccgatg acttatgact atatggggca aatatggtta cgccagtaag catcagcaat
      121 tacatatctc ttcctgatga ttttcccgct cgtaatattg cacctcaagt aaaagaagtt
      181 ttaaaggatt ttattgatgc actcagtacc ataatatgtg atgaagagtg gcgcacgtct
      241 ttaaacacca actcagccac aaaaaagata tttaacaacc ttgataactt atcatatatt
      301 cagaggactt cttttcgggg taacgacaca ctatacaatg aaaaggttca gtttaaactc
      361 acttatcccg tcaagaatgg aagacacaaa gaaaatattg aatttcaggt agtaataaat
      421 ttaagcccta tttagataat tttcgccatg atggagaaat taatattttt tgcgctccca
      481 acccaaagcc tgtcactatg gggcgcgtat ttcagaccgg cgtcgagcgt gtgctctttc
      541 tgtttatgaa tgattttatt gaacaatttc caatgatcaa ccttggcgcc cccatcaaaa
      601 gagcgcatac accacatatt gaacccctgc ctccggatca ccataccgct gcagattact
      661 tacgccagtt tgatttgctt gtcctgaatt ttatctctcg cggtaatttt gtcatactcc
      721 cccgattatg gaataactct gaggttcaca gatggttcgt caataaagat cctaacttga
      781 tcaccgccat tctcgacata acggacagcg aattaaaaga ggatttattg caaagcctga
      841 tggattcatt aggttctaac aaacatgtac aacccgaagt ctgcatctgc ttcttatccc
      901 ttttagcaga acaagaatct cctcattttc aggacttgtt tttatttttc gccaatatgt
      961 tattgcacta tcaccaattt atgaatccca atgaaagtga tctgaatgac gtgttgatgc
     1021 cagcatcatt aagtgatgat aaaattatca aacatatggc gcgcaggacc ctcaaactgt
     1081 ttgtaaaaaa tgaaacgccg ccaaaagtta ctcacgaaga cctggtgaaa aacaggcctc
     1141 gctcccctgt cagaccacct atacccgcaa ccgccaaaac gccagacctc cctgaacgtc
     1201 attaaaccgt gatgttaccg actctctgac gcggaacaga atcagcgtca gagaaacgga
     1261 aaacgcgatc cagatcacaa atgcattgta ttcacatcat taaccgtttt aagatcattt
     1321 catcactttt tcgcaactca cccgataatc tgttatgaca acaaacactg tttcccgcaa
     1381 agtggcgtgg ctacgggtcg ttacgctggc agtcgccgcc ttcatcttca acaccaccga
     1441 atttgtccct gttggcctgc tctctgacat tgcgcaaagt tttcacatgc aaaccgctca
     1501 ggtcggcatc atgttgacca tttacgcatg ggtagtagcg ctaatgtcat tgccttttat
     1561 gttaatgacc agtcaggttg aacggcgcaa attactgatc tgcctgtttg tggtgtttat
     1621 tgccagccac gtactgtcgt ttttatcgtg gagctttacc gttctggtga tcagtcgcat
     1681 tggtgtggct tttgcacatg cgattttctg gtcgattacg gcgtctctgg cgatccgtat
     1741 ggctccggcc gggaagcggg cacaggcctt gagtttaatt gccaccggta cagcactggc
     1801 gatggtctta ggtttaccgc tcgggcgcat tgtggggcag tatttcggct ggcgaatgac
     1861 cttcttcgcg attggtattg gggcgcttgt tacccttttg tgcctgatta agttacttcc
     1921 cttactgcct agtgagcatt ccggttcatt gaaaagcctc ccgctattgt tccgccgccc
     1981 ggcattgatg agcatttatt tgttaactgt ggtggttgtc accgcccatt acacggcata
     2041 cagctatatc gagccttttg tgcaaaacat tgcgggattc agcgccaact ttgccacggc
     2101 attactgtta ttactcggtg gtgcgggcat tattggcagc gtgattttcg gtaaactggg
     2161 taatcaatat gcgtctgcgt tggtaagtac ggcgattgcg ctgttgctgg tgtgtctggc
     2221 actgctgcta cctgcggcga acagtgaaat acacctcggg gtgctgagta ttttctgggg
     2281 gatcgcgatg atgatcatcg ggcttggtat gcaggttaaa gtgctggcgc tggcaccaga
     2341 tgctaccgac gtcgcgatgg cgctattctc cggcatattt aatattggaa tcggggcggg
     2401 tgcgttggta ggtaatcagg tgagtttgca ctggtcaatg tcgatgattg gttatgtggg
     2461 cgcggtgcct gcttttgccg cgttaatatg gtcaatcatt atatttcgcc gctggccagt
     2521 gacactcgaa gaacagacgc aatagttgaa aggcccattc gggccttttt taatggtacg
     2581 ttttaatgat ttccaggatg ccgttaataa taaactgcac acccatacat accagcagga
     2641 accccatcag gcgggagatc gcttcaatgc cacccttgcc caccagccgc ataattgcgc
     2701 cggagctgcg taggcttccc cacaaaataa ccgccaccag gaaaaagatc agcggcggcg
     2761 caaccatcag tacccaatca gcgaaggttg aactctgacg cactgtggac gccgagctaa
     2821 taatcatcgc tatggttccc ggaccggcgg tacttggcat tgccagtggt acaaaggcaa
     2881 tattggcact gggttcatct tccagctctt ccgacttgct tttcgcctcc ggtgaatcaa
     2941 tcgctttctg ttgcggaaaa agcatccgaa aaccgataaa cgcgacgatt aagccgcctg
     3001 caattcgcag accgggaatc gaaatgccaa atgtatccat caccagttgc ccagcgtaat
     3061 acgccaccat catgatggca aatacgtaca ccgaggccat caacgactga cgattacgtt
     3121 cggcactgtt catgttgcct gccaggccaa gaaatagcgc gactgttgtt aatgggttag
     3181 ctaacggcag caacaccacc agccccaggc caattgcttt aaacaaatct aacattggtg
     3241 gttgttatcc tgtgtatctg ggttatcagc gaaaagtata aggggtaaac aaggataaag
     3301 tgtcactctt tagctaacct tgcatcgcat tgaccaaaac ttgaaccgat ttagcaaaac
     3361 gtggcatcgg tcaattcatt catttgactt atacttgcct aggcaatatt atcccctgca
     3421 actaattact tgccagggca actaatgtga aaagtaccag cgatctgttc aatgaaatta
     3481 ttccattggg tcgcttaatc catatggtta atcagaagaa agatcgtctg cttaacgagt
     3541 atctgtctcc gctggatatt accgcggcac agtttaaggt gctctgctct atccgctgcg
     3601 cggcgtgtat tactccggtt gaactgaaaa aagtgttgtc ggtcgacctg ggagcactga
     3661 cccgtatgct ggatcgcctg gtctgtaaag gctgggttga aaggttgccg aacccgaatg
     3721 ataagcgcgg cgtactggta aaacttacca ccagcggcgc ggcaatatgc gaacaatgcc
     3781 atcaattagt tggccaggac ctgcatcaag aattaacaaa aaacctgacg gcggacgaag
     3841 ttgcaacact tgagcatttg cttaagaaag tcctgccgta aacaaaaaag aggtatgacg
     3901 atgtccagac gcaatactga cgctattacc attcatagca ttttggactg gatcgaggac
     3961 aacctggaat cgccactgtc actggagaaa gtgtcagagc gttcgggtta ctccaaatgg
     4021 cacctgcaac ggatgtttaa aaaagaaacc ggtcattcat taggccaata catccgcagc
     4081 cgtaagatga cggaaatcgc gcaaaagctg aaggaaagta acgagccgat actctatctg
     4141 gcagaacgat atggcttcga gtcgcaacaa actctgaccc gaaccttcaa aaattacttt
     4201 gatgttccgc cgcataaata ccggatgacc aatatgcagg gcgaatcgcg ctttttacat
     4261 ccattaaatc attacaacag ctagttgata acgtgacaac gtcactgagg caatcatgaa
     4321 accactttca tccgcaatag cagctgcgct tattctcttt tccgcgcagg gcgttgcgga
     4381 acaaaccacg cagccagttg ttacttcttg tgccaatgtc gtggttgttc ccccatcgca
     4441 ggaacaacca ccgtttgatt taaatcacat gggtactggc agtgataagt cggatgcgct
     4501 cggcgtgccc tattataatc aacacgctat gtagtttgtt ctggccccga catctcgggg
     4561 cttattaact tcccaccttt accgctttac gccaccgcaa gccaaataca ttgatataca
     4621 gcccggtcat aatgagcacc gcacctaaaa attgcagacc cgttaagcgt tcatccaaca
     4681 atagtgccgc acttgccagt ccgactacgg gcaccagtaa cgataacggt gcaacccgcc
     4741 aggtttcata gcgtcccagt aacgtccccc agatcccata accaacaatt gtcgccacaa
     4801 acgccagata catcagcgac aagatggtgg tcatatcaat agtaaccaga ctgtgaatca
     4861 tggatgcgga accatcgaga atcagcgagg caacaaagaa gggaatgatt gggattaaag
     4921 cgctccagat taccagcgac atcaccgccg gacgcgttga gtgcgacatg atctttttat
     4981 tgaagatgtt gccacacgcc caactaaatg ctgcagccag ggtcaacata aagccgagca
     5041 tcgccacatg ctgaccgttc agactatctt cgattaacac cagtacgcca aaaatcgcta
     5101 aggcgatccc cgccaattgt ttgccatgca gtcgctcccc gaaagtaaac gcgccaagca
     5161 cgatagtaaa aaacgcctgt gcctgtaaca ccagcgaagc cagtccagca ggcataccaa
     5221 agttaatggc gcagaacaga aaggcaaact gcgcaaaact gatggttaat ccatacccca
     5281 agagcaaatt cagtggtact ttcggtcgtg caacaaaaaa gatggccgga aaagcgacca
     5341 gcataaagcg caaaccggcc agcatcagcg gtggcatgtt atgaagcccc actttgatga
     5401 ccacaaaatt tagcccccat acgaccacta ccagtagcgc caacacccca tcttttcgcg
     5461 acattctacc gcctctgaat ttcatctttt gtaagcaatc aacttagctg aatttacttt
     5521 tctttaacag ttgattcgtt agtcgccggt tacgacggca ttaatgcgca aataagtcgc
     5581 tatacttcgg atttttgcca tgcgtccgat ggttaataac aacgacgagg caagaagggc
     5641 gctggtagag cgtcgtaggg ataagttcat ttgtctgtcc ggcagtgcgt ttcgttatgt
     5701 tttattttgg agatgtaaag aaatagcatg gctacgctgc catttatgac catttacttg
     5761 agtcgccagt acagcctgag tgtcgatcta atcggttatg cgatgacaat tgcgctcact
     5821 attggcgtcg tttttagcct cggttttggt atcctggcgg ataagttcga caagaaacgc
     5881 tatatgttac tggcaattac cgccttcgcc agcggtttta ttgccattcc tttagtgaat
     5941 aacgtgacgc tggttgtgct cttttttgcc ctcattaact gcgcctattc tgtttttgct
     6001 accgtgctga aagcctggtt tgccgacaat ctttcgtcca ccagcaaaac gaaaatcttc
     6061 tcaatcaact acaccatgct aaacattggc tggaccatcg gtccgccgct cggcacgctg
     6121 ttggtgatgc agagcatcaa tctgcccttt tggctggcag ctatctgttc cgcgtttccc
     6181 atgcttttca ttcaaatatg ggtaaagcgc agcgagaaaa tcatcgccac ggaaacaggc
     6241 agtgtctggt cgccgaaagt tttattacaa gataaagcac tgttgtggtt tacctgctct
     6301 ggttttctgg cttcttttgt aagcggcgca tttgcttcat gcatttcaca atatgtgatg
     6361 gtgattgctg atggggattt tgccgaaaag gtggtcgcgg ttgttcttcc ggtgaatgct
     6421 gccatggtgg ttacgttgca atattccgtg ggccgccgac ttaacccagc taacatccgc
     6481 gcgctgatga cagcaggcac cctctgtttc gtcatcggac tggtcggttt tattttttcc
     6541 ggcaacaacc tgctattgtg gggtatgtca gctgcggtat ttactgtcgg tgaaatcatt
     6601 tatgcgccgg gcgagtatat gttgattgac catattgcgc cgccaggaat gaaagccagc
     6661 tatttttccg cccagtcttt aggctggctt ggtgccgcga ttaacccatt agtgagtggc
     6721 gtagtgctaa ccagcctgcc gccttcctcg ctgtttgtca tcttagcgtt ggtgatcatt
     6781 gctgcgtagg tgctgatgtt aaaagggatt cgtgcaagac cgtgggggca gcccgcgctt
     6841 tgttgattta agtcgaacac aataaaaatt taattcagcc ttcgtttagg ttatctctgc
     6901 taatatcttt ctcattgaga tgaaaattaa ggtaagcgag gaaacacacc acaccataaa
     6961 cggaggcaaa taatgctggg taatatgaat gtttttatgg ccgtactggg aataatttta
     7021 ttttctggtt ttctggccgc gtatttcagc cacaaatggg atgactaatg aacggagata
     7081 atccctcacc taaccggccc cttgttacag ttgtgtacaa ggggcctgat ttttatgacg
     7141 gcgaaaaaaa accgccagta aaccggcggt gaatgcttgc atggatagat ttgtgttttg
     7201 cttttacgct aacaggcatt ttcctgcact gataacggat cgttgacaca gtagcatcag
     7261 ttttctcaat gaatgaggtg tactggcaat agcggacact accatttgtt ctttttttaa
     7321 gcagccatct gatgatattt ttccctgaag gctgccgggg agatattccc cagacgagag
     7381 tgacgacgct gacgattgta gaaaatctca atgtattccc gtattactga gatggcttca
     7441 tcccggttat taaaacgata gtggctcagg ctctcatttt tcagcgttcc ccagaagctt
     7501 tccatcggag cattgtcgta acatgctgca attactgcta taaccaggct ggcctggcga
     7561 tatctccagg ccagccattg gtggtgttta tatgttcaag ccacgatgtt gcagcatcgg
     7621 cataatctga ggagccttac cgcgccattg tcgatgcagg cgttccagat cttcgctgtt
     7681 acctctggaa aggatcgcct cgcgaaaacg ctgcccattt tcacgcgtta atccgccctg
     7741 ctcaacaaac cactggtaac catcatcggc caacatttgc gtccacagat aagcgtaata
     7801 acctgcagca tatccgccac caaaaatatg ggcgaaataa ctgctgcgat agcgtggcgg
     7861 tatagcagga agatccatat tttccgccac cagcgcccgc aattcaaaat catcgacatc
     7921 ctgcattgct tcgttttctt ccaggcaatg ccagcgcata tcgagaagtg cggcgctaag
     7981 cagttcgctc atctcatacc ctttgttgaa caggctggca ttacgcattt tctgttgcag
     8041 ttcgtcaggc attgctgccc cgctctgata atgccgggcg tagcgagcga atacctgcgg
     8101 atgcgttgcc cagtgttcgt tgatttgcga cggaaattcg acaaaatcac gcggcgtgtt
     8161 ggtgccggaa agcgtggcat aacgctggcg ggcaaaaagg ccgtgcagcg tatgaccaaa
     8221 ttcatggaat aaggttatta catcatccca gagtaacaac gcaggctcac cggcagcggg
     8281 tttctgataa ttgcagacgt tataaattac cggatgcgtt tcattaagcg ttgattgctc
     8341 aacaaaattg cccatccatg caccgccgct ttttgaatca cgggcgaaga aatcaccgta
     8401 aaataacgcc agtcccacgc cattatgatc aaaaatttcc cacacacgaa cgtcaggatg
     8461 gtagaccgga atatcaaaac gttcgacaaa cttaataccg aagagctgat tcgcggtcca
     8521 gaatacacct tcatttaaca ccgtgtttaa ttcaaaatat ggcttgagct gcgcctcatc
     8581 aagatcaaat ttctcccgcc gtacctgttc cgcataaaat gcccagtccc acggctgcgc
     8641 gctaaacccg ccctgctgct tatcgataac cgcctgtatg gaggctaatt catcgctcgc
     8701 acgttgacgc gccgctggaa caatttcccg cataaagttg agtgctgctt ctggcgtttt
     8761 tgccatctga tcggcgattt tccatgcggc ataatgagga aaaccaagca gtttcgcctg
     8821 ctgtgcgcga atctctacca gacgttgaat gatagcgcgg gtatcattgg catcattttt
     8881 ttccgctcgc gtccagcccg caataaacag tttttcacgc gtcgcacgat cgcgcatttc
     8941 ggcaagcgcc ggttgttggg tggtattcag cagcggaatc agccatttgt tatccagacc
     9001 tttctcgcga gccgcctctg ccgccagcgc aatctcttgc tcactcattc ctgccagctg
     9061 cgcgatatcg ttcacaacca gaccgccgga tttatttgct gcaagtaacc gctggttaaa
     9121 ctggctggtc agggtcgcag cttctgtatt cagtactttt aattttgctt tatcagcttg
     9181 tgcaagtttg gctccggcaa ggacaaaacg ttgatgaata acctccacca ggcggatgga
     9241 ttcactatca agccccaggg attcacggcg ctgccagaca gcatctaccc gcgcgaataa
     9301 ttcaccgttc agatagatat cattagccag ttccgccagt tcagcggaaa actgttcatc
     9361 aagacgctgt aattcatcat tggtatgcgc cgcagtcatc gcaaaaaaga cgctggtaac
     9421 gcgggtaatt aattctccgc tttgttccag tgccagaata gtattgttga aatcaggcgt
     9481 ttgcgggtta agcgcgatgg cagcaatttc tgcccgcttt tgttgcattc cctcatcgaa
     9541 tgccgggcga tagtgatgat tggcaatttg atcaaaatga ggagccagat acggcagtgt
     9601 gctttgcaca aggaaaggat tcattgttgt cattttcttc tcctgaacgc gaggtgttcc
     9661 atagcgtagg cttactgata acggagtgca atcttgcaaa ccagtattac ccgctcttaa
     9721 gcatcacgtg ctatgttatt gacacacaaa agcgttgagg aacagtgaga tgatcgtttt
     9781 agtaactgga gcaacggcag gttttggtga atgcattact cgtcgtttta ttcaacaagg
     9841 gcataaagtt atcgccactg gccgtcgcca ggagcggttg caggagttaa aagacgaact
     9901 gggagataat ctgtatatcg cccaactgga cgttcgcaac cgcgccgcta ttgaagagat
     9961 gctggcatcg ctccctgccg agtggagcaa tattgatatc ctggtaaata atgccggcct
    10021 ggcgttgggc atggagcctg cgcataaagc cagcgttgaa gactgggaaa cgatgattga
    10081 taccaacaac aaaggcctag tatatatgac gcgcgccgtc ttaccgggta tggttgaacg
    10141 taatcatggt catattatta acattggctc aacggcaggc agctggccgt atgccggtgg
    10201 taacgtttac ggtgcgacga aagcgtttgt tcgtcagttt agcctgaatc tgcgtacgga
    10261 tctgcatggt acggcggtgc gcgtcaccga catcgaaccg ggtctggtgg gtggcaccga
    10321 gttttccaat gtccgcttta aaggcgatga cggtaaagcg gaaaaaacct atcaaaatac
    10381 cgttgcattg acgccagaag atgtcagcga agccatctgg tgggtgtcaa cgctgcctgc
    10441 tcacgtcaat atcaataccc tggaaatgat gccggttacc caaagctatg ccggactgaa
    10501 tgtccaccgt cagtaatttt tatacccggc gtaactgccg ggttattgct tgtcacaaaa
    10561 aagtggtaga ctcatgcagt taactcactc acaagcaaga acgaatgacc gtcgaaacgc
    10621 aacttaatcc cacacagcct gtcaatcagc agatttatcg tattcttcgt cgcgacattg
    10681 tccattgcct gattgcacca ggcacaccgt tgtcggaaaa agaagtttct gttcgtttca
    10741 atgtgtcacg ccagccggtt cgtgaagcct ttattaaact ggcggaaaac ggcctgattc
    10801 aaattcgtcc gcagcgtggc agctacgtca acaaaatttc catggcccag gtgcgcaacg
    10861 gcagttttat ccgtcaggcc attgagtgcg cggtggcgcg tcgggcggcg agcatgatta
    10921 ccgaaagcca gtgctaccaa ctggaacaaa atcttcacca gcaacgcatt gccattgagc
    10981 gcaagcaact ggatgatttt tttgaacttg atgacaactt ccatcaactc ctgacgcaga
    11041 ttgccgactg tcaactggcg tgggatacca ttgagaacct gaaagcgacc gttgatcgcg
    11101 tgcgctatat gagtttcgac catgtttctc caccagaaat gctgttacgc cagcatcttg
    11161 atattttctc tgccctgcaa aaacgtgatg gcgatgcggt agaacgtgca atgacgcaac
    11221 atttgcagga aatcagcgaa tccgtgcgcc agatccgcca ggaaaacagc aactggttta
    11281 gcgaagagta attcatttac tctcatccca tccgggatga gaggcttttc cccccgcctt
    11341 acggctcatg catgcatcaa aaaagatgtg agcttgatca aaaacaaaaa atatttcact
    11401 cgacaggagt atttatattg cgcccgttac gtgggcttcg actgtaaatc agaaaggaga
    11461 aaacacctat gacgacctac gatcgtaacc gtaacgcaat caccactggc agccgtgtta
    11521 tggttagcgg caccggtcac actggcaaga tcctgtcgat tgatactgaa ggtctgaccg
    11581 ctgagcaaat ccgccgcgga aaaaccgtag ttgttgaagg ttgtgaagag aaactggcac
    11641 ctctggacct gattcgtctc ggcatgaact aagtgtgtga atgccgccga tggcggcatt
    11701 gcttttttta cttcacggaa tatttcgcca cggtcgcttt cgcgccatgc gctaataaag
    11761 acaagtacgc ttccgtcact tttgcagtaa acaagctatt gtctggcaaa tcatcaccaa
    11821 agattgcctt aatcgccagc aatgactgga cgcgcgcttt cccttcggca ctactttgta
    11881 cagccttctg aataacaggt aacagtgggt cactgatttc tatcggattt ccctgttcat
    11941 caacaccacc gacataacgc atccaacccg cgacgcccag cgccagcaga tcgaacttgc
    12001 tgtcatgcgc cagatgccag cgaacagaat ccaacatccg ctgtggcaat ttctggctgc
    12061 catccatcgc aatctgccag gttcgatgac gtaacgccgg gttgctatag cgtgcaatta
    12121 atcggttagc gtaatcttgc aaatcaacgc cctgcacttt cagcgtcggc gcttgttcct
    12181 gcaacatcat gccatacgcc gcatgacgat aatgttcatc ttccatacag tcattaatgt
    12241 gctgatatcc agcaagataa cccagatatg ccaggaatga atgactgccg ttgagcatgc
    12301 gcaacttcat ctcttcataa ggcagcacat cactaaccag ttcggctccc gctttttccc
    12361 attccggacg tccggctaca aagttatctt cgataaccca ctggcggaaa ggttcacagg
    12421 caacgccagc aggatcgcgc acaccggtaa gttgttcgat tttcgccagc gtatcctctg
    12481 ccactgcggg cacaatacgg tccaccattg ttgatgggaa agtcacgtta tcttcgatcc
    12541 attgtgccag ttttacatca acggcttgcg cgtaggaagt gacaacgtca cgcataacat
    12601 gaccgttttc tggcatgttg tcacatgaca tgacggtaaa tgcgggaagt cctgccgctt
    12661 tacggcgagc cagcgcctca acaatcaccc ctgttgctgt tttcggctgg tggggatttt
    12721 gcacgtcggc agcgaccatc gggtgatcga gcattaactg tccggtcgcc ggagagtgga
    12781 aatacccttt ttcggtgatt gtcagagaga caatcgcgat ttgcggttca cacatcgcag
    12841 ccaacacggt ttctaagcca tccatctgta cgtgcaaggc ttttttaacg acgccaacga
    12901 cgcgagccgt ccacgcatcg gccgacattt ccgcaacggt ataaagatta tcttgctgtt
    12961 gtaaatcggc aatttgctgt tcgccgccga ttaagttgac ctcataatat ccccagtcac
    13021 tgaaatgttc tgtagcaaga atatcggcat acacgccctg atgcgcacgg tgaaatgcac
    13081 caaagcctaa atgaacaatt cttggggcca ggttattacg atcataaaca gggagtgtcg
    13141 cttttgctga taacaaatta tttcccataa caattcctta aatttaaata tggcaagcta
    13201 tatgttttgt tatatgaata aa
//
LOCUS       DADMEQ010000110        12749 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.111, whole genome shotgun sequence.
ACCESSION   DADMEQ010000110 DADMEQ010000000
VERSION     DADMEQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.111"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(101..739)
                     /gene="rutR"
                     /locus_tag="JNZ05_003155"
     CDS             complement(101..739)
                     /gene="rutR"
                     /locus_tag="JNZ05_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HAY8566287.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSCDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1027..2118
                     /gene="rutA"
                     /locus_tag="JNZ05_003156"
     CDS             1027..2118
                     /gene="rutA"
                     /locus_tag="JNZ05_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HAY8566288.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPSAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2118..2810
                     /gene="rutB"
                     /locus_tag="JNZ05_003157"
     CDS             2118..2810
                     /gene="rutB"
                     /locus_tag="JNZ05_003157"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HAY8566289.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGDYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIHHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKVALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2822..3208
                     /gene="rutC"
                     /locus_tag="JNZ05_003158"
     CDS             2822..3208
                     /gene="rutC"
                     /locus_tag="JNZ05_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HAY8566290.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGLVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3216..4016
                     /gene="rutD"
                     /locus_tag="JNZ05_003159"
     CDS             3216..4016
                     /gene="rutD"
                     /locus_tag="JNZ05_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HAY8566291.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAVRAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4026..4616
                     /locus_tag="JNZ05_003160"
     CDS             4026..4616
                     /locus_tag="JNZ05_003160"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HAY8566292.1"
                     /translation="MNEAVSPGVLSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4627..5121
                     /gene="rutF"
                     /locus_tag="JNZ05_003161"
     CDS             4627..5121
                     /gene="rutF"
                     /locus_tag="JNZ05_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HAY8566293.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5142..6470
                     /gene="rutG"
                     /locus_tag="JNZ05_003162"
     CDS             5142..6470
                     /gene="rutG"
                     /locus_tag="JNZ05_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HAY8566294.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6552..6659)
                     /locus_tag="JNZ05_003163"
     CDS             complement(6552..6659)
                     /locus_tag="JNZ05_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566295.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7032..7628
                     /gene="wrbA"
                     /locus_tag="JNZ05_003164"
     CDS             7032..7628
                     /gene="wrbA"
                     /locus_tag="JNZ05_003164"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY8566296.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7649..7876
                     /locus_tag="JNZ05_003165"
     CDS             7649..7876
                     /locus_tag="JNZ05_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566297.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7914..9155)
                     /gene="agp"
                     /locus_tag="JNZ05_003166"
     CDS             complement(7914..9155)
                     /gene="agp"
                     /locus_tag="JNZ05_003166"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HAY8566298.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9448..10704)
                     /locus_tag="JNZ05_003167"
     CDS             complement(9448..10704)
                     /locus_tag="JNZ05_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HAY8566299.1"
                     /translation="MGRNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPRISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10965..11885
                     /gene="cbpA"
                     /locus_tag="JNZ05_003168"
     CDS             10965..11885
                     /gene="cbpA"
                     /locus_tag="JNZ05_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HAY8566300.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFFEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11885..12190
                     /gene="cbpM"
                     /locus_tag="JNZ05_003169"
     CDS             11885..12190
                     /gene="cbpM"
                     /locus_tag="JNZ05_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HAY8566301.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12283..12699)
                     /gene="torD"
                     /locus_tag="JNZ05_003170"
                     /pseudo
     CDS             complement(12283..12699)
                     /gene="torD"
                     /locus_tag="JNZ05_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
BASE COUNT         3051 a         3243 c         3209 g         3246 t
ORIGIN      
        1 ttcgctgagt gtgaataccg ctgccgctgg cggtaacgcc agtttaatga cgatcggtta
       61 aacaggtagc cggaggatgt tacaacctcc tccggcatct ttaacgtggt cgaatcccct
      121 caataataat ccgctgcacg ttttcaaccg tttgattgaa aaatacctca tcgcgcaacg
      181 tcgcgcctgt caccgcctcc acctgagggg cgaaatcggc gtaatgttga gtggaagccc
      241 aaatcataaa aatcaaatgc tgcggatcaa tcggcgcgag tttgccgctt ttgacccaac
      301 cggcaatcag cgccgatttc tcatcaatta atgccttcaa atcgcccgtc agttcatcca
      361 ttaacaacgg cgcgcctgcc agcatctcca tacagaacag gcgcgaagcc tgcggataat
      421 cgcatgagac ttccagcttc agacggatgt actcttttat cgccgccagc ggggcgaaat
      481 cttcacgaaa cgcttttaac ggtgccagcc agatatcgag aatctgccgc agcacggcaa
      541 tatacagcgc ctcttttgac gggaagtaat acagcagatt ggtttttgaa acacccgcca
      601 actctgcgat ctgctccagc cttgtgccgt gaaaaccgaa ttgtgaaaaa gtgtccagtg
      661 ctgcgctaag aatcgctttt ttcttcgcgc ttactgcgcg cgaacgttta cccgttgttt
      721 tcactgcgcc ttgcgtcatg cgctctcccc tcttgtagat tggtaacagg atagcaaaac
      781 ccccgcccgg cctcgacctt ctgcactctc atcgcgcacg gatgcatgtt ttatgtgcaa
      841 ctgttttgac cgtttagtcc actttttacc agatatttta gataacacaa tgctaacaca
      901 ttaataacat tcacttttta aaaactggca tccgctttgc aaacaagcca atacgcggct
      961 gaaaaggata tgcaggatgc agcgccccgt ctgactttca ctttacgaga cgaagagagg
     1021 ttgatgatga aaattggcgt attcgtacct attggcaaca acggctggct catttcgacc
     1081 cacgcgccgc agtacatgcc gacctttgaa ctgaataaag ccattgtgca aaaagcggag
     1141 cattaccatt tcgatttcgc cctgtcgatg atcaaactgc gtggctttgg cggcaaaact
     1201 gagttctggg atcacaacct tgagtcgttc accttgatgg cgggtctggc agccgtgacc
     1261 tcgcgcattc agatttacgc cactgctgcc accttaacgt tacctccggc aatcgtcgcc
     1321 cgtatggccg caaccatcga ctccatctct ggcgggcgtt ttggcgtcaa cctcgtgact
     1381 ggctggcaaa agcccgagta tgagcagatg ggtatctggc ctggcgatga ctatttctcc
     1441 cgtcgttacg actatctcac cgaatatgtt caggtgctgc gcgacctgtg gggcacgggg
     1501 aaaagcgatt ttaaaggcga ttttttcacc atgaatgatt gtcgcgtcag tccgcaaccg
     1561 agtatcccca tgaaagtgat ctgcgccggg caaagcgacg ctggcatggc gttctccgcc
     1621 cagtatgccg atttcaactt ctgtttcggc aaaggcgtaa atacacccac ggctttcgcc
     1681 ccgaccgctg cgcggatgaa acaggccgca gagcaaaccg gacgcgacgt tggctcttat
     1741 gtgttgttta tggtgattgc cgatgaaacc gacgatgcca ctcgcgccaa atgggaacac
     1801 tacaaagcgg gcgcggatga agaggcgtta agctggctaa ccgaacaaag tcagaaagat
     1861 acccgctccg gtactgacac caacgttcgt cagatggccg atcccacttc ggcggtaaac
     1921 atcaatatgg ggacgttagt cggttcttac gccagtgtcg cgcgcatgtt agatgaagtc
     1981 gcaagcgtgc ctagtgcaga aggcgtgctg ttgaccttcg atgattttct gtcgggaatc
     2041 gaaaccttcg gcgagcgcat tcaaccactg atgcagtgcc gcgcccatct ctctgcgctg
     2101 actcaggagg tggcatgatg acaaccttaa ccgctcgacc ggaagccatt accttcgatc
     2161 cgcagcaaag tgcgcagatc gtggtggata tgcaaaacgc ttatgccacg ccaggcgact
     2221 acttagatct cgccgggttt gatgtctcaa ccactcgccc ggtcattgcc aacattcaaa
     2281 ccgctgtgac cgcagcgcga gcggcaggga tgttgatcat ctggtttcaa aatggctggg
     2341 atgaacagta tgtcgaggct ggcggccccg gctcaccgaa ttttcataaa tcgaacgccc
     2401 tgaaaaccat gcgtaagcag ccgcagctgc aggggaaatt gctggcgaaa ggctcctggg
     2461 attatcaact ggtggatgaa ctggtgccgc agcctggcga tattgtgctg ccgaagccgc
     2521 gctacagcgg tttcttcaat acgccgctgg acagcatttt gcgcagccgt ggaatacacc
     2581 atctggtttt caccggtatc gctaccaacg tctgcgtcga atcgacgcta cgcgatggct
     2641 tttttctgga gcatttcggc gtggtgctgg aagacgcaac acaccaggcg gggccggaat
     2701 ttgcacagaa agtcgcgttg ttcaatatcg aaaccttttt tggctgggtc agcgacgtcg
     2761 aaacgttctg cgacgcgctt tctcccacgt cctttgctcg tatcgcttaa ggagtttaac
     2821 gatgccaaaa tccgtaatta ttcccgctgg cagcagcgca ccgctggccc ccttcgttcc
     2881 cggcacgctg gctgatggct tggtgtatgt ctccggtacg ctggcttttg atcaacataa
     2941 taacgtgctg tttgccgatg acccaaaggc gcaaacccgc cacgttctgg aaactatccg
     3001 caaggtgatc gagacggcgg gtggcacgat ggcggatgtg accttcaaca gcatctttat
     3061 taccgactgg aaaaattacg ccgcgattaa cgaaatctac gccgagtttt ttccgggtga
     3121 taaaccggcg cgattctaca ttcagtgcgg actggtaaaa cctgacgcgc tggtggaaat
     3181 cgccacaatt gcgcatatcg ccaagtgagg ccgcgatgaa actttcactc tcacctcccc
     3241 cttatgctga tgcacccgta gtggtgttga tttcgggtct tggcggtagc ggcagttact
     3301 ggttaccgca actggcggtg ctggagcagg agtatcaggt agtctgttac gaccagcgcg
     3361 gcaccggcaa taatcccgac acactggcag aagattacag tatcgcccag atggcagcgg
     3421 aactgcatca ggcgctggta gccgcaggga ttgagcgtta cgcggtggtc ggccatgcgc
     3481 tcggtgcgct ggtggggatg cagctggcgc tggattatcc cgcgtcggta actatgctgg
     3541 tcagcgttaa cggctggcta cgaataaacg cccatacgcg ccgctgtttt caggttcgcg
     3601 aacgattact gtatagcggc ggcgcgcaag catgggtgga agcgcagccg ttgttcctct
     3661 atcccgccga ctggatggcg gtccgcgcac ctcgcctgga ggcagaagac gcgctggcac
     3721 tggcgcattt tcagggcaaa aataatttac tacgtcgact taacgccctc aaacgcgctg
     3781 actttagtca ccatgcggat cgcatccgct gcccggtgca aatcatctgc gccagtgatg
     3841 atctgctggt gccaacagca tgttccagtg aacttcatgc cgccctgccc gatagccaga
     3901 aaatggtgat gccctatggc ggacacgcct gcaacgtgac cgatcccgaa acgtttaatg
     3961 ctctgttact caacgggctt gccagcctgt tacatcaccg tgaagccgcc ctgtaaggaa
     4021 ttgctatgaa cgaagccgtt agcccaggtg tgcttagcac cctgttcacc gatgcccgca
     4081 ctcacaacgg ctggcgggag acacccgtca gcgatgagac gttacaggag ctttatgccc
     4141 tgatgaaatg ggggccgaca tcagctaact gttctccggc acggatcgtg tttatccgca
     4201 ctgcagaagg aaaagaacgt ctgcgcccgg cgctttccag cggcaatctg caaaaaaccc
     4261 tgaccgcgcc cgtcaccgct atcgtcgctt gggacagtga attttatgaa cggttaccac
     4321 tactgtttcc ccacggtgat gcccgcagtt ggtttacctc cagcccacaa cttgccgaag
     4381 aaacagcgtt tcgcaacagt tccatgcagg cggcctatct gatcgtcgct tgccgtgcgc
     4441 tgggactgga taccggcccg atgtcgggtt ttgaccgtca acacgttgac gacgcctttt
     4501 ttgcgggcag cacgctgaag agcaatctgc tgattaatat cggctatggc gatagcagca
     4561 aactttttgc gcgcctgcca cgtctgtcct ttgaagaagc ctgcgggctg ttgtaaggag
     4621 caaactatga acatagttga tcaacaaacc tttcgcgatg cgatgtcctg tatgggcgcg
     4681 gcggtcaata tcatcaccac ggacggtcca gccgggcgcg ccgggttcac cgccagcgcc
     4741 gtctgcagcg tgaccgacac gccgcccaca ttactggtgt gcctgaatcg tggggcgtcc
     4801 gtctggccgg tattcaatga aaatcgaacg ctgtgtgtaa atacactgag cgccgggcag
     4861 gagccgcttt caaatctttt tggcggcaaa acgcccatgg aacaccgctt tgccgccgcc
     4921 cgctggcaga cgggtgtgac cggatgcccg caactggaag aggctctggt ttcgtttgac
     4981 tgccgtatca gccaggtggt gagcgtcggc acccacgaca ttctgttttg cgccatcgaa
     5041 gcgattcatc gtcacaccac accctacggg ctggtgtggt tcgatcgcag ttatcacgcg
     5101 ctgatgcgcc ctgcttgtta acctccttaa ggagacagct gatggcaatg ttcggttttc
     5161 ctcactggca gttaaaatcg acctctacag agagcggcgt ggtcgcgccg gatgaacgat
     5221 tgccgtttgc tcagacggca gtaatgggcg ttcaacacgc ggtggcgatg tttggcgcga
     5281 cggtgttaat gccgattctg atggggctgg atcccaatct ttccatttta atgtcgggga
     5341 tcggcacgtt actgtttttc tttattaccg gcgggcgcgt ccccagttat ctcggctcca
     5401 gcgcggcttt tgtcggcgtg gtgattgccg cgacgggctt taacggtcag ggcattaacc
     5461 cgaatatcag catcgcgctg ggcgggatta tcgcctgtgg gctggtttat accgtcattg
     5521 gcctggtggt gatgaaaatt ggtacacgct ggattgaacg actaatgccg ccggtggtga
     5581 cgggtgcggt ggtgatggcg attggcctga acttagcgcc tatcgcagtg aaaagtgtat
     5641 cggcttcggc ctttgatagc tggatggcgg taatgacggt gctgtgtatt gggctggtgg
     5701 cggtgtttac ccgtggcatg atccagcggt tattgattct cgtaggatta attgtcgcct
     5761 gtctgctgta tggcgtgatg accaacgttt tagggctggg aaaagccgtc gattttactc
     5821 tcgtcagcta tgctgcctgg ttcggtctgc cgcatttttc cactcctggt tttaatggcc
     5881 aggcgatgat gttgattgcg cccgtggcgg tgattctggt ggcggaaaac ttaggtcatc
     5941 tcaaggcggt cgccgggatg accggacgca atatggaccc gtatatgggg cgagcattcg
     6001 tcggtgatgg tctggcaacg atgctttccg gctctgtcgg cggtagcggc gtcaccacct
     6061 atgctgaaaa tatcggtgtg atggccgtga caaaagtcta ctcgacgctg gtgtttgttg
     6121 cagctgccgt catcgctatg ctgttgggct tttcgccgaa atttggcgcg ctgatccata
     6181 ccattccggc cgcggtgatt ggtggtgcat caattgttgt gttcgggctg attgccgtcg
     6241 caggcgcaag gatatgggta caaaaccgtg tcgatttaag ccagaacggt aatttgatta
     6301 tggtcgcagt aaccttcgtt ctgggcgcgg gtgattttgc tctcacgctt ggcggtttta
     6361 cgttgggagg gatcggtaca gcaacctttg gcgcgatttt actcaatgcg ttattaagca
     6421 gaaggttggt tgacgttcca ccgccagaag ttgttcatca ggagccataa acagaaaacc
     6481 gccgggttac cggcggtgat gactttttga cgagcttaag aaaaaaatgc attcgtcatt
     6541 gattagcccg gctagttgtc gcttttgccg tgactgctct tgccaccttt tttacctgct
     6601 tctgatgctc tttcgcggtc ttctgcaaaa ttgccggaac cgcctcgatg gtttgccatt
     6661 actgacctcc gtctgggtat agacatcata ttgcatagaa ttaacgcggc gtatggcggc
     6721 tgccatattt tcgctacgtg ggactaagct tagtgaactg acgcgggcag acccgcgttt
     6781 ggtaatatcc tgcaacagct tcttagcgag aatagatatc atctccaata attatccata
     6841 agccgctctt atgattaaaa aaacgcgatg agataaaaat gtgtcatttt gcgacaaaat
     6901 tacgtgcttg tgaaagttgt tataaatcaa ataagtggtt gtgaaatttg cactctgaaa
     6961 aggacgtctt atctttaaat aagtggtagc gaatcgctac ggaatagaga taacacgagg
     7021 agtggttaga aatggctaaa gttctggtgc tttattattc catgtacgga catattgaaa
     7081 cgatggcacg cgcagtcgct gagggtgcaa gcaaagtgga tggtgcagaa gttgtcgtta
     7141 agcgtgtacc ggaaaccatg ccgccgcaat tatttgaaaa agcaggcggt aaaacgcaaa
     7201 ctgcaccggt tgcaaccccg caagaactgg ccgattacga cgccattatt tttggtacac
     7261 ctacccgctt tggcaacatg tccggtcaaa tgcgtacctt cctcgaccag acgggcggcc
     7321 tgtgggcttc cggcgcacta tacggaaaac tggcgagcgt ctttagttcc accggtactg
     7381 gcggcggtca ggaacaaacc atcacatcca cctggacgac ccttgcgcat cacggcatgg
     7441 tgattgtccc cattggctac gcagcgcagg aattatttga cgtttcacag gttcgcggcg
     7501 gtacgccgta cggcgcaacc accatcgcag gcggtgacgg ctcacgccag ccaagccagg
     7561 aagaactgtc tattgctcgt tatcaagggg aatatgtcgc aggtctggca gttaaactta
     7621 acggctaatc ttcaacagga ggatacgcat gccaactcaa gaagcgaaag ctcatcacgt
     7681 cggtgaatgg gcatctctgc gcaatacgtc gccggaaata gccgaggcca ttttcgaagt
     7741 cgccgggtac gacgaaaaaa tggcagaaaa gatttgggaa gaaggtagtg atgaagtgtt
     7801 agtcaaagcc tttgctaaga ccgataaaga ttcgcttttt tggggcgaac agaccatcga
     7861 acgtaaaaac gtttaaccag cgactccccc gcttctcgcg ggggagtttt ctgttatttc
     7921 accgcctcat tcaacacgct atcaaactta tccatcgggc agaaaccgtt ggcatctatc
     7981 gggcaaccgc ttaattccag cgtcacgcgc tgcgcaggtg cctgcagggt taacgcatcg
     8041 gcattacgta actgttccgc actctggtac acatattcaa ttttcatcaa atcgcgattg
     8101 gctttgctgt catgccaacg ctggaaaacg attttgccgc caatcggcgt gcgttcgttc
     8161 tggtcatgca actgatacgg tttgaaatcc agcgccgtta acagagaggc tatgttggag
     8221 tcgtgcccaa ctaacaccgt gattttcgga gcactggcgc gatcggtgac cagggctttg
     8281 tcgatataac tgaccagcgg tttcgcaaca ttgcgcgcca cttccggtga ggtaaacaga
     8341 ctgtcctggt aaccgttttt cagcttcgac aataccttcc actgctggtc agatttgatt
     8401 tctccccagg ccacctgatc catcggaaaa ccttcgtagt attgcaaagt aaacgcatct
     8461 accagcgagt tgccgacttt cagcggcccg gaaacacctg gttcttgttg atacttggcg
     8521 ctaaaggtat ttttgccatc caccagcgaa cactgttgtt tctctttaca ggcaggggaa
     8581 tctttatagt taacgatttt ttccagtagc tggtagctgt cggtaagctg gagtttgctg
     8641 agctctttct ccattgccgc caccgcctgt tcactgaatg cggcggaatc atcggtgatc
     8701 atcgggttaa aggttgggtc catagtgccc attttttcct ggtgatgcac aggaatatca
     8761 cacccaggga atgcaccagt aataaagaac tgggcggtag cgacggtacg ttgcagacta
     8821 ttggcatagg cataaacggt gtccggcggc gggcattccc ccgatttcac tatcccctgc
     8881 tctgccagcc attcacgcat gtaatggccc atatacactt cgagcacgcc acctttggtg
     8941 gtgagttgcc caccggggac gtcccattcg ggccatttat tcggcgtcga ctgctccagc
     9001 acactgccat tgttcgccag cggcgcacgt aagttatggc ggctcatcat gagcacttgc
     9061 tgtagctgat agccttccgg tacggtttgt gcctgagcat ttgaagcgag taaaactatc
     9121 cctgccacag ttgcggcgat tagcgttttg ttcattcctg gcacctcttt tgttatcaat
     9181 cattaaagag tgtgacagaa atatgacttt tttccgggaa caggttcgca aaatggcttg
     9241 ttgaacgcct gtttttctat atacatcttc aaaaacaggc aaggtttgac gcaaactatg
     9301 cctgattagc ccctgcctcg cgtacgccgc tgataattgc acctgtggct aaacagaata
     9361 tctgcggcgt taccaataca gagacaatat ttaccaataa aaaagtgtga gactaataac
     9421 aatggttttt agcatatgcc tttgccatta agttgcataa cgatttaaat agtcaagttg
     9481 cgcattcatt tccttatttt ttaacttata agcctgagaa aaaagttcag gagggaggta
     9541 aacatcataa tgattgccag tgtttattaa ataaccatac tcaagatctt catctgttga
     9601 aaaggattca tttcccaagg agattgatgc cacattacta ttaacaataa ataatgagca
     9661 aatgctatct gaaagaacag caagaaaatt cttcaccgct ttaatcctat ccgacgtgtc
     9721 ggtatctaaa gcaagcaaat tcttgttatc attaaatata aactcgggta aatgcagtat
     9781 gcattttttg tcattcaagc aactatcaat gaattgtttt ttaaaaaaca gaaagctcca
     9841 tgacataaaa ctacccacac cctgaatttt gttgtcagat tgcagatcaa cgccaaataa
     9901 tgtctttaca tcttccctga gattatagaa tgccgtatca acaagagcct cgatcgtttg
     9961 tttatcaaca attttaataa actcttcgta tacttgcttt tgcgtcttat ggctgcccag
    10021 cgcccatgaa tactcatgtt cttttaaaac cagtatggct cttaacagac aatatccatc
    10081 gccatctact ggagcgtatt ttaatccata tgttttatta atttcactct gaataaaaga
    10141 tatgccagac aaatcaatac cagtatctaa atcagatatt ctcggcatat ttatctgttc
    10201 tggctggata ctcatatttt catcatctaa acaatactca gaaaaaacat catcacacgc
    10261 attaatgttt tcaatatatt ccataaagcc ataccaataa taacctttaa aatctgtgat
    10321 agactctgaa cctctgcccg gcaaggacac tctaacaata tcaacatctt gaacatattg
    10381 tatagtatat gtaatacctc ctgaaacccc tgaataaatt tgtgtatcat tagtacgcaa
    10441 tgttctcata aataatgata gatcattatc attctgcatt tcctcaaatt ctttttctag
    10501 tgattcacat tccagacatg gagtatcctc ttcatttact ttacgtctga gttcattgta
    10561 caaccgcata aaacacacca gtgtgttttc aaaaagcgtg attgaatatg tttgattttt
    10621 ttcgtactca ttttttatat cgttccaggc ctgtttcaaa taattatttg cagtgcaact
    10681 aattccatgt atattacgac ccatatatag cgtctataaa atttaataaa taatgacgcc
    10741 ctagttaaac ttaaagtgcc tggttcaact atcaaaaatc gctcaccctt tttcacctgt
    10801 ttaaaatatg ttcagcaacc catcttgatg gcgacctcct ctccgcgatg atttcaataa
    10861 catattctgt gttggcatat gaaattttga ggattaccct acacttatag gagttacctt
    10921 acaggggttc cttcaatttg tgttgattta cgcgagataa cgctatggaa ttaaaggatt
    10981 attacgccat catgggcgtg aaaccgacgg acgatctcaa gacaatcaag accgcctatc
    11041 gtcgactggc ccgtaaatac catcctgatg tcagcaaaga accggatgcc gaagcccgct
    11101 tcaaagaggt cgctgaagcc tgggaagtgt taagtgatga acaacgtcgc gctgagtatg
    11161 atcagatgtg gcaacatcgc aacgatccgc aatttaaccg tcagttccat catggcgacg
    11221 gtcagagttt taatgccgaa gattttgacg atatcttctc gtcaattttc ggtcagcatg
    11281 cccgccagag ccgtcaacgc cccgccgcac gcggccacga tattgaaatc gaagtggcgg
    11341 tattcttcga agaaacgctt actgagcata agcgtaccat cagctataac ctgccggttt
    11401 ataacgcctt tggcatgatc gaacaggaaa tcccgaaaac gctgaatgtg aagatcccgg
    11461 caggcgtcgg caatggtcaa cgcatccgcc tgaaaggcca ggggacaccg ggcgaaaacg
    11521 gcggtccaaa tggcgatttg tggctggtga ttcatattgc gccacatccg ctgttcgata
    11581 ttgtcggcca ggatctggaa attgtggtgc cggttagccc gtgggaagcg gcgctgggta
    11641 ctaaagtcac cgttccaaca ctgaaagaaa gcattttgct gactatcccg ccaggcagcc
    11701 aggccgggca acgattgcgc gttaaaggca aaggtctggt gagcaaaaaa cagaccggcg
    11761 atctgtatgc ggtactgaaa atcgtgatgc cgccgaaacc ggatgaaaac actgccgcac
    11821 tgtggcagca actggcagac gcccagtcgt cttttgatcc acgtaaagat tgggggaaag
    11881 cataatggct aatgttacgg tgacttttac tattaccgaa ttttgcctgc ataccggcat
    11941 ctctgaagag gagttgaatg aaattgtcgg tttgggggtg gttgaaccgc gtgagatcca
    12001 ggaaacaacc tgggtatttg acgaccatgc cgccattgtg gtgcaacgcg cggtacgcct
    12061 gcgtcatgaa ctggctctgg actggccggg gatcgcggtg gcgctgacgt taatggatga
    12121 tattgcgcac ctgaagcagg aaaaccgcct gctacgccag cggctttccc ggtttgtagc
    12181 tcatccgtga tgtgtgtttg ccggatgtga tgacgcatcc ggcaactgcg ccataatgcc
    12241 tgatgcgacg ctaaacgcgt cttttcaggc gcataaacga cgctatctgt tttggtggtc
    12301 gcactccact aacaccagca ataactggct tagtgccgcg taaaaaccaa agctgtcata
    12361 ctgtcgacaa cgcgcagcaa actctggtaa ccattgccgt agcgccgtca gtgttttttg
    12421 ccgcaaactg tcgattcttc gcgcaggaac ggtcccctct cccagcgaaa aatgcagata
    12481 actcaacaac tcgagataga tcgccagatg atctgccggt tcgttgaaat tgccactggt
    12541 ttccatccct gcctcaacta acaagcgttt aatctcttgc tcgtcctgtt tgtaggccga
    12601 tgcatacggc agcgccgctt gtttgtcggt catcagaaac aggccgcaaa agtcagcggc
    12661 cagttccaga cgggcatcgt cacgtactgt cagcgtggca ggtaatgctg ccaacttact
    12721 gatttagtgt atgatggtga ttttaaggt
//
LOCUS       DADMEQ010000111        12505 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.112, whole genome shotgun sequence.
ACCESSION   DADMEQ010000111 DADMEQ010000000
VERSION     DADMEQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.112"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(67..2016)
                     /locus_tag="JNZ05_003171"
     CDS             complement(67..2016)
                     /locus_tag="JNZ05_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY8566302.1"
                     /translation="MTDSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDATAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2101..2437)
                     /locus_tag="JNZ05_003172"
                     /pseudo
     CDS             complement(2101..2437)
                     /locus_tag="JNZ05_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
     gene            complement(2437..2793)
                     /locus_tag="JNZ05_003173"
     CDS             complement(2437..2793)
                     /locus_tag="JNZ05_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8566303.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2793..4289)
                     /locus_tag="JNZ05_003174"
     CDS             complement(2793..4289)
                     /locus_tag="JNZ05_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8566304.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4286..4447)
                     /locus_tag="JNZ05_003175"
     CDS             complement(4286..4447)
                     /locus_tag="JNZ05_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005288483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HAY8566305.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(4457..5019)
                     /locus_tag="JNZ05_003176"
                     /pseudo
     CDS             complement(4457..5019)
                     /locus_tag="JNZ05_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5016..5540)
                     /locus_tag="JNZ05_003177"
     CDS             complement(5016..5540)
                     /locus_tag="JNZ05_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566306.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRYARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERQRHWTQELLSS
                     ELQRSLRPVKRKHK"
     gene            complement(5506..5922)
                     /locus_tag="JNZ05_003178"
     CDS             complement(5506..5922)
                     /locus_tag="JNZ05_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY8566307.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            complement(5919..6242)
                     /locus_tag="JNZ05_003179"
     CDS             complement(5919..6242)
                     /locus_tag="JNZ05_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY8566308.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            complement(6287..8017)
                     /locus_tag="JNZ05_003180"
     CDS             complement(6287..8017)
                     /locus_tag="JNZ05_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY8566309.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGHGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNGEVETLNGK
                     LTELENLKSDLEAELAEVKRPAGGTQNKVAGEHKEAFIGFMRKGREDGLRELERKALQ
                     VGNDEDGGYAIPEELDRTILTLLKDEVVMRQEATVITLGGSDYKKLVNLGGTTSGWVG
                     ETEARQETATSKLGLIEPFMGEIYGNPQATQKMLDDAFFNVEDWINSELALEFAEQEE
                     IAFTSGDGSKKPKGFLAYESTDEDDKTRAFGKLQHIASGAASGVTADAIIKLIYTLRK
                     AHRSGAKFMMNNSSLFAIRLLKDNDGNYLWRPGIELGQPSSLAGYGIVENEQMPDIAA
                     DAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQAIKLMKI
                     GAATRQKAAA"
     gene            complement(7995..9236)
                     /locus_tag="JNZ05_003181"
     CDS             complement(7995..9236)
                     /locus_tag="JNZ05_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY8566310.1"
                     /translation="MFFQGLFQRKNNTPVTTPGMLAEELGLSYDTYTGKRISSQRAMR
                     LTAVYSCVRVLAESVGMLPCSLYKISGTLKTRAVDERLHKLISAKPNGYMTPQEFWEL
                     VIVCLCLRGNFYAYKVKALGEVVELLPIDPGCVEPKLNSQWQPVYQVTFPDGSVDVLT
                     QDEIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFGNGAVTSGVLRTEQKL
                     TPDAYERMKRDFEERHLGLGNAHRPMILEMGLDWKPVALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRESKQGKFY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(9236..9418)
                     /locus_tag="JNZ05_003182"
     CDS             complement(9236..9418)
                     /locus_tag="JNZ05_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566311.1"
                     /translation="MFLIILTPLAGVLGALLLSYGTWLIYPPAGFVVAGALCLCWSWL
                     VARYLDRGHRVASGGE"
     gene            complement(9430..11187)
                     /locus_tag="JNZ05_003183"
     CDS             complement(9430..11187)
                     /locus_tag="JNZ05_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY8566312.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEEWGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSSGIGLYMTGADGEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQV
                     SARVNFFTKHMNVWVTAESAWMDMIKWEKCEYIAPRHELKTYPMWVGVDLAHKIDICA
                     AAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQI
                     KSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLV
                     YAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNKSTPEAKIDGPVAMFTAMSRMLVNG
                     GEPEPDLSEHLVSVGIRSL"
     gene            complement(11187..11669)
                     /locus_tag="JNZ05_003184"
     CDS             complement(11187..11669)
                     /locus_tag="JNZ05_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAY8566313.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDAKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEGYTYRTETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRVKVNIAGPDDVDPLAELLKARD
                     "
     gene            complement(11817..12167)
                     /locus_tag="JNZ05_003185"
     CDS             complement(11817..12167)
                     /locus_tag="JNZ05_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY8566314.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(12210..>12505)
                     /locus_tag="JNZ05_003186"
     CDS             complement(12210..>12505)
                     /locus_tag="JNZ05_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAY8566315.1"
                     /translation="QRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQS
                     VREATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
BASE COUNT         2824 a         3714 c         2894 g         3073 t
ORIGIN      
        1 aaacaaactc cattgttagc atgttacgcg ataattttgc ctcatctttc accacaaaaa
       61 aagaactcac cttttaaatg gagaataccc aacatcatgg gtgattttca taaagggatc
      121 gcctgttttc ggcaaatcga taacgcgcat tccaggcggc gcgttgtcaa acgtcacctt
      181 aagttcgctg cgggttgccg gtgaagatgc aaggcttaac agctgattcc tttccagtga
      241 tacattcggg gtgtagcgtt tatcgggaga taaataccca tcaccacgtg gcatctgcca
      301 tccggtcatg tcatagacaa aatcatggaa ctgtttgccc cactcgtcga acttatcatt
      361 cagaccaaat ccactgttca gtgcatcagc cacaaaattt ttaacaagcc agggatgttc
      421 tttttcaaac tcctgcgcca tcatgcccag ttcaagcaat ccaccaatca gactgatctt
      481 tccgaggccc tttaatttca gccctgtttt tcctgccgcc gttgtccatt gattctgggc
      541 gactgttgcc gcacgaatac ttgccaccgc cttcacacca atatacaggg cagaaaccgt
      601 tgctattgtt tttactgcct gttcccatcc ccccattgct ctgacaacct gatcaacctc
      661 ctgccagact tttttcacaa ccggaccgac cgtttcccag ttatcaatta tgagatatgc
      721 accaccgacg agaagcgcaa ttaatccctt tgccggcgtc atattcatca cgctacccat
      781 gatttttgtt atgcgtgtca atgtgccaat cgccacgccc atcgtcagta atgccgcacc
      841 agttttcgcg atggttttta cgacctcagg attttctctg acaaacgtgc gcacttcttc
      901 cagaaaaggc ttcatctctt ttatgccttc attgagtgaa ggcaggaagg tttcccccag
      961 cgtggaagaa atcgcattga tctggttctg cagcagcaac aactgatttt ctgtcgtcgc
     1021 ggcacgggcg gcatattctt tctgcattga gccgccatac tgctgcgcat ccgccacacg
     1081 attaaagttg gttcgcaaca aatccagatt cgtgagcagc ggcgcaatgg cccccaggga
     1141 ttccttcccg aacagcgctt tcagcacagc agcctgtttt tctttcggca cttttgccag
     1201 agaatccagt acgtgcagca tggccccgcg ggcgtctttc tgcatatccg ccgccagttt
     1261 tttcgggcta atgcgcaacg agcggagcac ttttttctgc gatttggtgg cagaatccct
     1321 cgctgtcagc gacaacataa aattctttat ccccgtcgca gcaatttctg actccacccc
     1381 cattccggca atggttgccc ccattgcggc aatctctccg gaggccacac ccgcaacact
     1441 gcctaaaggg ccaatacggg tcacaacatc agaaatcttt ttcgcacttg caggaccggt
     1501 attgccaaga tagttaatct tatccgcaag ccctgccacc tctccctgtg tcagtttaaa
     1561 ggcagtgcgc cactgcgcca tcatctgacc ggattcttct gccgtggcgt caaaggccac
     1621 gcccatcttc acggcatcgt cagtaaactg catcagctcg tcacgggcga taccagcctg
     1681 accacccgcc gccacaattt cggcgatacc ttccgccgac atgggcagtt ctgttgacag
     1741 gtcgcgcacc tgctccgtca ttgccttaaa cgcttccggc gtatccagac catccaccac
     1801 tttccggaca tccgccattt ttgattcaag ggcaatggca gatttgaccg ggagcgccag
     1861 cgcccccagt actgcggttc cggcacccgc tgcgcccagt gacaggctgg caaactcctt
     1921 tttaaaacct ttcagctggc gctgcattcc cttcagcggt gccgatacct tatccacggc
     1981 agtgatgatc gccttcagct gaaaactgtc agtcatgctt catctcctcg ttaatgcgga
     2041 cggcctcggc ttccagttct gtaaactgcg aaacagccac ccgtcgcagc tccagtggat
     2101 tcagtttcca gaaccaggca acattgtaga gtcgtttccc ggaggtgctt cccgtctccg
     2161 actgggtaaa aaaacgcagg atctgcatgc tggtcttaaa aatatccagt tttgccatct
     2221 gcgccgccga tgaacgcggg atccccgcca gcaacgggat atatttcagt gccacctggc
     2281 tgtccagttt aatactgcct tcaccggaaa taatgaaagg aaaacccagc gcctcgattt
     2341 cgtcatacgt gggctcgcgc aactccagca catgcagcat ttcgttatgc gctgtcaccg
     2401 gtttttttaa aactatttct gtaacgttca ttgccattac tgataccctc cctcttcgcc
     2461 gtggaattca agatctgccg tgccttcttc ggcattatga ttagcctctc cgtgcagcca
     2521 tgccgccgaa agcacataca ccatgccgtt tgccagctca gcggtaatcg tcatctggtc
     2581 tgaggtggta attttgtcga ccggaaagtt tttgggcact ttaaacgtgc ccttaacgta
     2641 aggtgaccgc caggtctcct tgtaatccac atcccctgcc atgccgacga tgtcatcccg
     2701 gacattggtg ttcatcggca cttcaatgcc acctgtcagc gataactgct gaccatccac
     2761 tttaaaaaaa caggttccgg cgatctttgc cattatgctg actcctctga atactgaaga
     2821 cggaactggt taaccaccgc aaagacacgt aactgattaa cataatccgg cgggaacagc
     2881 gtattcagtc ggttcgggtt atctgcatca cgctcaacta tcagatactg tttaaacaga
     2941 tcgtaatttt ccacaatacc ggcacgctcc atctgacgat atgtcgccag aagttcccct
     3001 ttgataacgg caggagtgac aatcgcctgc cccggaccaa aacgggtacc atcatttgcc
     3061 agcttgtgac gtccgtactt gcttgtgatg accgatttca gtttgcgaag aacatatgca
     3121 ctggtatgca gagtttcact gtccagatag ctgttgtctg ccacgccata cgcattcttt
     3181 ttgtaggtgg ttacggaacg ctggatctgc aacgtgccgc cttccacata agccgtcgcc
     3241 acgccgtgag ataaaagggt ctgctgctct gtcatgatga atcgcttacc tttcggtgcc
     3301 ggaagcatcc ccaccagctc ccccgtctgt gtcggacggg caggatcatt ccggataaat
     3361 accgcttcac gggcaaggcg actggcaacc agttcatcga caggcgactg ggtttctttt
     3421 tcgtaaccgg caagcgtgat atgttgctga ttatgcatat ctccggcacc aaccagctct
     3481 gacagcgttc ccagttttgc ggtatagaca tgcccgtata actggcgcgc ataactccag
     3541 cgaccgctgc tgtcattcat ttcggtcatc atcatattga tggaggcggc atcgttgaac
     3601 ggcagaccga taaagtcgaa tgcctcatcg cccatagcag caacagcggc ggtaagatca
     3661 ggcgctccgc taccggcagc accgacttcc gtcaccaccc gaagccctgc tggcagaatc
     3721 tcaccaccac cagaaccata ataattcaga cagaccggta actcattacc atacagcccc
     3781 ttatgacggg cagtcagcgt caccacgcct gcatcagatg acgccgtaaa cggcagggtg
     3841 ataaccccgt ttaccgcttc cttaatcgcg gtggcaaccg cagtggcatc atcgccattc
     3901 accacaggca cctgtacacg ggagcgcccg acatacagac tcagggtgcc gctttcctct
     3961 gcttctccgg caaccgtcac cctgaccgtc gccgccgccc ctctggcttc cggtactgca
     4021 ataacataca gttcaccgaa aggatctgtc tgacggtaga cttccaccat acgcgccagc
     4081 tggctccccg ccccacaaat ctggcgggca taatctgccg atggcatcag caccaggctg
     4141 ttaacctcaa tggctgcatc gttgctggca tgcccgatca gtaatgcagg cgcgctggtc
     4201 accgctgtat ttgccgcaga attgttcatc tcggcgtaga acaacggcac cagcgtattc
     4261 gacggaacag tattaaaact tatcgtcatg attttttagc cttatcctgt ttaacacgta
     4321 ccacatcacc cgccgctata cggcggaacc agtagctgct ttcttccaca tttcgccctt
     4381 cggaaggcaa aaggtctcca cgggcagggt caggaaccga ccgccctttc aggggtttca
     4441 caaacatgag ggactcttaa ttctgaggga aacgcatttc cagatggtgc tcaatgtcac
     4501 catccgggcc agtaccagga tcgatgtaat ccacatcaat actcagcagg gataaatccg
     4561 gcaggcattc acgtcctctg cctgtcgcgt gtcttcttcc gtgatttcat acttcgccgt
     4621 aaaatcaaac tggtaataca gttcgtaacg gttcagatcc agcagggtgc caccgacata
     4681 aacaatctcg cccccttgcg ggtccggcat ccatccaagc agggctttcc agagttctct
     4741 ccggacatca tgaacggcgt cataggctgc ccactgccct ttttcatcac gctcattgct
     4801 gagcaccaca atcacggaga agccttcagt caaatcctgc cagtaatcgg tctgtgattt
     4861 ttgttccccc ggcgcatcgt cagagggaac cacataggca gcaggcaggc gaagttttcc
     4921 ggcatcaggg atcgccttaa actgcgccgc gccaccaaca cgctcctcaa aacgcgggca
     4981 tcgctcacgc agcgccgcaa ttatcgttgt cagtttcatt tatgcttcct ttttaccgga
     5041 cgtaacgaac gctgcagctc actggacaac agttcctgcg tccagtgacg ctgacgctca
     5101 ataacgtcag ccataaagtt attacgcggg gccagccgga aagtcgaaga atggtgcttc
     5161 ttctgccgct tatccttttt atccattcca tacgctgaat ggcgaacgcc gtaatacaga
     5221 aacgccggat aataaggagc accttcagga aaacggcgat tcccctgccc gtttttctgg
     5281 tcaggggaaa ttttcaccat cagtccgggg cgacgcgtcg tttttttggg aacgtaataa
     5341 ccaatggagc gggccagacg cccggtctga tatcccgggt tctctcccgg accggagcgt
     5401 ccgcgtttaa tcaccagacg tctggcataa cgcatgtaaa cgcgcccgat ttgcacaaac
     5461 gcccgacgca gacgggcgcg attaaactcc agctcctctg gttgtttaaa gtcgacgtgt
     5521 aaaaatgctg tctgattcat ggcattcacc ccgtcgtcgc gctgtacgca gttcttcaca
     5581 ttccagtaat aaaaaacgcc gctgaccgtt caggtcacgt attcgccgga tccggtactc
     5641 ttggccgtaa taaaccactt cgtgatctgc cgtgatgtcg tgccggaaac ggatcgtaaa
     5701 ataatgcgta acgatatttt ctgtctgcac tgagccctga taagcggcag cgcctggctg
     5761 agccaccttt gcccagacat caaacgactc cggatacgtt ggctccgtac caaaatcagc
     5821 ggtgggttca tccacacgaa ggcggatctt tatccggcga ttcagctctc cgggatccgg
     5881 taaaaggtaa gtggcactgg tctgactttg cctgattttc atagcgggac aatcctgtaa
     5941 gggccagcaa gccatctgaa actcattggc gtttccagtt tctccacatc ggtgatcgtt
     6001 gagcgatttt cataaaaatg actgaccagc atcagcattg ccagacgaac gtcatcagtc
     6061 agatgcatcc cgtcaggatc atcttccgga atcgtctctt ccggtgcata caacttcctg
     6121 ttcaggtatg tctctgtttt tttctgtacc gcctgtgcca gcagtttcag aaaatcgccg
     6181 tcactgtaca aaccatcatc gagccggaga tgagatttaa tctcctcttc tttcaggagc
     6241 atattttcct cctgtgcccg cgattacgcg ggcacaaaaa accgcattac gcagcggctt
     6301 tctggcgggt tgcagcccca attttcatca gcttaatcgc ctgagaatcc accagcatac
     6361 cgccggttcg cttggtggta taaaaaccca cgaacggttt gttggtgtac gggtcacgca
     6421 ggatacgggt accgatgcga tcaacgatgg tatagccgcg tttgaagttc ccaaacgcaa
     6481 tggcttttgc atcggcggca atatccggca tctgctcatt ctcaacgatg ccataccctg
     6541 ccagagaaga aggctgaccc agctcaatac ccggacgcca caaataattt ccgtcgttat
     6601 ccttcagcag acgaatggca aacaggctgc tgttgttcat catgaacttc gcgccgctgc
     6661 ggtgcgcctt acgcaaggtg taaatcagtt taatgatcgc atcagcggtc acgccggaag
     6721 ccgcgccgga agcaatgtgc tgaagtttgc caaacgcacg ggtcttgtca tcttcatcgg
     6781 tggactcata agccagaaaa ccttttggtt ttttgctacc gtcgccactg gtaaaggcaa
     6841 tttcttcctg ttcggcaaat tccagcgcca gctcactgtt gatccagtct tccacattaa
     6901 agaaagcatc atcgagcatt ttctgtgtgg cctgcgggtt gccgtagatt tcccccataa
     6961 agggctcaat cagtcccagt tttgaggtgg cggtttcctg acgcgcttcc gtttccccca
     7021 cccatccgga cgtcgtgccg cccagattca ccagtttttt ataatccgag ccacccagag
     7081 tgattacggt ggcctcctgg cgcatcacca cttcatcttt cagcaacgtc agaatggtgc
     7141 gatccagttc ttccggaatg gcataaccac catcttcatc attacccacc tgcagtgcct
     7201 ttcgctcaag ctcacgcagg ccatcttcac gccccttgcg cataaaaccg ataaacgctt
     7261 ctttatgttc cccggcaact ttattttgcg taccacctgc cggacgctta acttcagcca
     7321 gctcagcctc aagatcgctt ttgagatttt ccagttcagt cagttttccg ttgagggttt
     7381 ccacttctcc gttcagtgca gagcccacat cctcagcgtc gcgcagggac agtgcgccat
     7441 agcccccggc catgaatgct ttggcctggg tacgggagag tccgacatca cgcaggactc
     7501 tttcgatttt tttctgttcg gggatttccc cgcgggccag tgcgttcttg acgtcgctga
     7561 tccgcgcctc gtcgttagac gggaacgtca ccagactgac ttcccagagg tcgatttctt
     7621 tcagcagaaa ggcttctttg ctccggtcgt attcccagtc tttcaggacg tacccaatag
     7681 aaaggccggt taacgaaccg gccttcatgt gtgcatgtgc acgttttgcg aggggatcat
     7741 catcaataag caaccgtccc ctgacgtaaa gcccgacatc gtcttccttc atttcggtgt
     7801 aaacaccgat gggttcatcc atgcggtgct gccagagcag cgcaggtaac gcttttctgt
     7861 cactccacgc ccgcagggaa gcagcaaatg ccccggacat caccacatca tcgtggctgt
     7921 cctttacacc aaagacggag ccatgccctt caaactcacc ggagtcactg acagatttca
     7981 gactcagcgg tacatcaaga cgttgtttcg tctgcattgg cgttatcctt ctgcttaccg
     8041 gctttactgc catcggaggg tttcgtggtc atgttcatcg gtgtgagata gacatcccca
     8101 ccgggacgcg gattcatatc ttccaggtcg cggcagtcat tgggagagta aatcccccag
     8161 ttaatcccgg tggcgtaggc ttcaaaacgg gatttcatat ccccgcgcag taacgccccg
     8221 gcgttaaatt tggcgtaaaa cttcccctgt ttgctctccc tgaccagccc tgtattgatc
     8281 cgttgttcaa tacgggtcag atacggcaca agggaatagt tgataaatcc gagccccagc
     8341 tcttcaatat tgttgaaagt ggcgcgatcg gtgttctgca ccatgtgcag cggcacgcgg
     8401 aaaagacgac agatttcttc caactgaaac ttgcgggttt ccaggaactg gctgtcctcg
     8461 gcattcagtg ccaccggctt ccagtccagc cccatttcca gaatcatcgg acggtgcgca
     8521 ttacccagcc caagatgacg ctcctcaaaa tccctcttca tgcgctcata agcatccggc
     8581 gtgagctttt gttccgtgcg caacacaccg gatgtcaccg caccattacc aaacaacctg
     8641 gcaccgtgct cctcggttgc cgctgccagt gaaatggcct cgcgcgcata tgcaatggga
     8701 ttcagcccga ccagtccgtc cagcgtcagg gtgcgcacat gccagatttc atcctgggtc
     8761 aacacatcca cggaaccatc cggaaacgtc acctgataaa ccggctgcca ctggctgttc
     8821 agcttcggtt ccacacagcc cggatctatc ggaagaagct ccacaacttc ccccagcgcc
     8881 ttcaccttgt aagcgtaaaa attcccccgc agacacaggc agacgataac cagctcccag
     8941 aattcctgcg gcgtcatgta gccattgggt tttgctgaaa tcagcttatg cagccgttca
     9001 tccaccgccc gtgttttaag ggtgccgctg attttgtaga gactgcaggg cagcatacca
     9061 acagactcag ccagcaccct gacgcaggaa tagaccgccg tcagccgcat ggcccgctgg
     9121 ctgctgatcc gctttccggt ataggtgtcg tatgacaacc ccagctcttc cgcaagcatc
     9181 ccgggcgttg tgacgggggt gttatttttg cgttgaaaaa gcccctggaa aaacattact
     9241 cacctccgga ggcgacccgg tgaccgcgat cgagataacg cgcaaccagc cacgaccagc
     9301 acagacacag cgccccggca acaacaaaac ctgccggggg ataaatcagc catgtgccat
     9361 acgacagcaa aagcgccccc agcacgcccg ccagtggcgt gagaattatc agaaacataa
     9421 tgacctcggt taaagcgagc gaataccaac gctgaccaga tgctcagaca gatccggctc
     9481 cggttcaccg ccattgacca gcatccggct cattgctgta aacatcgcaa cagggccgtc
     9541 gattttggct tccggcgtgg atttgttcgg gaagatattg tcgtttttgt ccggtttcac
     9601 cgtaacgtta gacatcatcc agttcatgac cgggtgatta ctgtgatgga aacgcccggc
     9661 atagaccagt gattccgttt ccttcatggc ctctgacaga ttgcggaccg tctgcggaac
     9721 ttccaccagc ggtatacctt cttcagccag cgccagactg aactgcattg cgctccacgg
     9781 gtcaaatccc agttccctca ggttttcacc gccaatccat tccagtaagt cactttttat
     9841 ctgagcatga tcgataacat caccatccgt cagaatcagc ttatccatct ccgcccactt
     9901 ccggtaaagt tctgcctgct gccgcgagca tcgttccagc cgtccttccg gaagccagaa
     9961 tttaaaatcg gcatgaacat gcccgttatc cgttcgccag agttttgccg ccgcacagat
    10021 atcaatctta tgagcaaggt caacgccgac ccacatggga tacgttttca gctcatgtcg
    10081 tggggcaatg tattcgcact tctcccactt aatcatgtcc atccaggcag actctgctgt
    10141 tacccacaca ttcatgtgtt tggtaaaaaa attcacccgc gcagagacct gttctttcgc
    10201 ttttttcgcc aggcgacgca gatcatccca gcgtttacag atgcccaggc cgggattcgc
    10261 tttctgccag accgtttcat caaacggatc atctccctca tcgagggtgt aaataatcgc
    10321 aaagtaggag tcgtctttta ccgcgccctc cacatcgctg ttatagccac gcaatacctt
    10381 gatggcgtaa tcacgctgct cgtaacaaat cccttccttg ttaaaccctg ccgtggtgat
    10441 accaaataaa agggactgca gacgggcacc ggttgccgtt tccagaacgt cccacacgtc
    10501 acgggtttta tgtgcatgca gctcatcaat aatggcgcag tggatgttca gaccatccag
    10561 gttgtttgca tctgaagaaa gcggttcaaa ttttgatgcg ctctgctcct gataaatcgc
    10621 cagcttgttg aaatcaaaca accgcccgag tgtcgaccgg gcttttctga ccatattttt
    10681 ggcgtcttca aacacgattc tggcctggtc acgcgtggtt gcggctgaat acacctcagc
    10741 cccgccttca ccatctgccc ccgtcatata cagaccgata cctgatgaca gggttgattt
    10801 tgcgttttta cgggcgactt cgttgtacgc tgtccggaaa cggcgcacca tcaccgggcg
    10861 tccgctgcca tcgctgcgca tgacaacttc cccggtctct tcattcacca gcggaatgac
    10921 aaaaccaaaa atattaatga ggataaacac atgccagtcc atcaactcaa tgggctgacc
    10981 tgccagcgcc ccttttacat ggggcacaaa tttgtagaaa ttcaggatgt gctgcgcacg
    11041 gggttcactg aaataaatcc cccactcttc gccgtacttc agatcatcaa gaaaacgctg
    11101 gcaggccaga cggacaaatt caccagcgac aatttctcct gcaacaacac gttcggcgta
    11161 gcggatcccg tcagccactt ttgccatcag tctctcgctt ttaaaagttc tgccagcgga
    11221 tcaacatcat ccggtccggc gatatttact ttcacccggc ttgccggtga cataccaaat
    11281 tctgcaagca tcgcccggat ccgcttccag gcatccgctt tcatcgcagc agccgggtgt
    11341 gccttaatca gcacatcacc gttctgcgtt tccgtgcggt aggtataacc ctcaacatcg
    11401 agggtttcgc agtgatgccg gtattcggtg taggcttcca ccagcaactc gagtgcacgc
    11461 gcatcaagct gagaaatgat cccttccgca ttcagctctt ccgccattcg cctgaaccag
    11521 tactttccct gcgcccctaa atgctgcgga attttaggga gacctttttc gtccttttta
    11581 gcggtttttt tggcgtcttt aacgggacgc tttgagggat tgcctcgtat caaatgcagg
    11641 cgtggcgggg ttttcggggg tcctgacata atcgatttta cctatcaatc gcttgatcgc
    11701 attcccaaaa aaaagttttc gaacctgcgg cgatgcgagg aagggttggc gggcggtccc
    11761 ggacagccag ggctgcaggg atttgacccg cccctcccta caagtgagaa taattatcac
    11821 ctgattcgtt cgcgcgctgt tttcgcttta tggcagggcc agcacagact ctgcaggttg
    11881 ctgtctgcgt ctgttccgcc atgcgctttc gggatgatgt ggtcgacggt tttcgcctcg
    11941 ctcaccacac cgacacgcag acacaactga cacagacctt tatcgcgctt cagaatacgg
    12001 gcacgaatca ccgtccattt tgagccatag ccacgctggt ggcggctcag tccgcgctgg
    12061 tgctgcaccc agccttcacc gcgatgctta tcgcagtaac ccgaactgtc tgtggttgta
    12121 cctgcacatc cacgcttacg gcaggcacgt gggattagtg atggcataaa tacctcatac
    12181 cctgcgaaat gtttaccacg ataaaaaggc tacttaatgc actgagtgcg gatatactcc
    12241 tgtgcccctt ccagttgcat ctgcatcgtc atcagccgct ctctgagggt gaaataatcc
    12301 cgttcagcgg tgtctgccag tcgggggctg gttgcattat ccacgccgga ggcggtggtg
    12361 gcttcacgca ctgactgaca gactgctttg atgtgcaacc gacgacgacc agcggcaaca
    12421 tcagcgcgca gagtttcatt ttcagctttc gcatcagcta actccttcgt gtatttagca
    12481 tcgagtgcat taacatcacg ctgtc
//
LOCUS       DADMEQ010000112        12294 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.113, whole genome shotgun sequence.
ACCESSION   DADMEQ010000112 DADMEQ010000000
VERSION     DADMEQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.113"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(1..1231)
                     /locus_tag="JNZ05_003187"
                     /pseudo
     CDS             complement(1..1231)
                     /locus_tag="JNZ05_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(1430..1514)
                     /locus_tag="JNZ05_003188"
     tRNA            complement(1430..1514)
                     /locus_tag="JNZ05_003188"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1478..1480),aa:Leu,seq:caa)
     gene            1710..2729
                     /gene="ahr"
                     /locus_tag="JNZ05_003189"
     CDS             1710..2729
                     /gene="ahr"
                     /locus_tag="JNZ05_003189"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HAY8566316.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2858..4372
                     /locus_tag="JNZ05_003190"
     CDS             2858..4372
                     /locus_tag="JNZ05_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HAY8566317.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGEKKISQT"
     gene            complement(4480..5562)
                     /gene="lptG"
                     /locus_tag="JNZ05_003191"
     CDS             complement(4480..5562)
                     /gene="lptG"
                     /locus_tag="JNZ05_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY8566318.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFILVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKDDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5562..6662)
                     /gene="lptF"
                     /locus_tag="JNZ05_003192"
     CDS             complement(5562..6662)
                     /gene="lptF"
                     /locus_tag="JNZ05_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY8566319.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6929..8440
                     /gene="pepA"
                     /locus_tag="JNZ05_003193"
     CDS             6929..8440
                     /gene="pepA"
                     /locus_tag="JNZ05_003193"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAY8566320.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8574..9017
                     /gene="holC"
                     /locus_tag="JNZ05_003194"
     CDS             8574..9017
                     /gene="holC"
                     /locus_tag="JNZ05_003194"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HAY8566321.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9017..11872
                     /gene="valS"
                     /locus_tag="JNZ05_003195"
     CDS             9017..11872
                     /gene="valS"
                     /locus_tag="JNZ05_003195"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAY8566322.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11926..>12294)
                     /locus_tag="JNZ05_003196"
     CDS             complement(11926..>12294)
                     /locus_tag="JNZ05_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HAY8566323.1"
                     /translation="RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRS
                     TLTYHGMLYRMCALVVISEITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQ
                     PDKGFLASISRGVMPSLPFL"
BASE COUNT         3031 a         3216 c         3305 g         2742 t
ORIGIN      
        1 tgaggtgagc atatcacctc tgttcaggtg gccaaattca gtgtgccact tcaaacatca
       61 acctacgctc cccaagatgt tccgccttat ggatgtaagc tgctcgcaca gagctacgct
      121 cctggtgact catctgccgc tctaccgcat ccctcgacca taatcccgac tcaatcaacg
      181 aactacaagc catcgtcctg aagccatgcc cacaaacctc gaccttcgtg tcatacccca
      241 ttacgcgcaa agccttgttc accgtattct cactcatcgg cttacgtgga tcgtgatcgc
      301 ccacaaaaat cagctttcga tttccgctca tgcttttgat cttttccaaa atacttaggg
      361 cctgccgcga caaaggtaca aggtgtggtg tccgcatctt cgagccacgc tgagaatgct
      421 tgactccttc cagttgctca cgctcacctg ggattgtcca catagccgtt tcaaaatcta
      481 cttctgacca gcgggcaaaa cgcagctcgc tcgaacggat gaaaaccaat aaagtgagtt
      541 caaccgccag tcgagttaac ggtctgccag aatagtgatc tatgcgatga agtaattcag
      601 gtatacggtt cagttcaaga gctgcacggt gctgtctttt cgccgtagca accgcacctg
      661 caatctcttg cgcggggttg tagtcgatta agccgctctg aacggcaaag cgcataatcg
      721 cggtagtacg ctgctgtaaa cgggcggcaa cttcgagccg cccggatgac tcgactgctt
      781 taatgggtac aagaagatcc cgcgtcttca gttccgcaat gttccgctta ccgatagcag
      841 taaagagatt atcctccaga cttttcaaaa cacgagcgct atgcgatgcc gaccacttct
      901 gattgctggc gtgccagtct ctggcgacta cttcaaacgt tatcgcctct tgttcctgtt
      961 ctaccttaac agctttcttg ttttcactgg gatcgacacc attagctaaa agcttacgag
     1021 cctcatcccg gcgtgccctg gcatccgcca acgacacttc ggggtatttc cccaatgcaa
     1081 gcatcttctc tttgccgcca aagcgataac gtagccgcca gtatttggag ccgttagggt
     1141 gaaccagcaa aaccatgcct tcaccgtcgg tcagcttata ggcttttgct tcaggcttag
     1201 ccgaacgaac cttcacatca ctcagagcca tgatgagtat cctttcaagg gttctgtgtg
     1261 ggtacaaaca ttatcgaacc gggaaatacc cgcagttgta cccgcatcag taagttgatg
     1321 tagattgaat caggttgact taggttgagt gaaaaagcga ggaaagcctt gcggatactg
     1381 gattttaggc acaaaaaaag acgtccgttg acgtctattg atgttccgat ggtgccgaag
     1441 gccggactcg aaccggcacg tatttctacg gttgattttg aatcaactgc gtctaccgat
     1501 ttcgccactt cggcactgaa gaggtatgcg gaaaacgttg tggattatac ctgtcgcacg
     1561 ccaccatgca agcgccagct tgcacccacc tcgctaagtg ctgaaatttt cagcattaca
     1621 ctcagcgata tgtaaccttt gtcacactcc aggccccccg ccctgccatg ctctacactt
     1681 cccaaacaac accagagaag gacccaaaaa tgtcgatgat aaaaagctat gccgcaaaag
     1741 aagcgggcgg cgaactggaa gtttatgagt acgatcccgg tgagctgaag ccacaagatg
     1801 ttgaagtgca ggtggattac tgcgggattt gtcattccga tctgtcgatg atcgataacg
     1861 aatggggatt ttcacaatat ccgctggttg ccgggcatga ggtgattggg cgcgtggtgg
     1921 cactcgggag cgccgcgcag gataaaggtt tgcaggtcgg tcagcgtgtc gggattggct
     1981 ggacggcgcg tagctgtggt cactgcgacg cctgtattag cggtaatcag atcaactgcg
     2041 agcaaggtgc ggtgccgaca attatgaatc gcggtggctt tgccgagaag ttgcgtgcgg
     2101 actggcaatg ggtgattcca ctgccagaaa atattgatat cgagtccgcc gggccgctgt
     2161 tgtgcggcgg tatcacggtc tttaaaccac tgttgatgca ccatatcact gctaccagcc
     2221 gcgttggggt aattggtatt ggcgggctgg ggcatatcgc tataaaactt ctgcacgcaa
     2281 tgggatgcga ggtgacggcc tttagttcta atccggcgaa agagcaggaa gtactggcga
     2341 tgggtgccga taaagtggtg aatagccgcg atccgcaggc actgaaagcc ctgtcggggc
     2401 agtttgatct cattatcaat actgtgaacg tcagcctcga ctggcagcct tattttgagg
     2461 cgctgaccta tggcggtaat ttccatacgg tcggtgcggt tctcacgccg ctgtctgttc
     2521 cggcctttac gttaattgcg ggcgaccgca gcatctctgg ttctgctacc ggcacgcctt
     2581 atgagctgcg aaagctgatg cgctttgccg cccgcagcaa ggttgcgccg acaaccgaac
     2641 tgttcccgat gtcgaaaatt aacgacgcca ttcagcatgt acgcgatggt aaagcgcgct
     2701 accgagtagt cctgaaagcc gacttctgat ccctttgccg ggtgctgtcg cccggcactt
     2761 aaatctcttt ggaaggcgtc tcgcaatctg ctgaatttag cggtgaagag tgcacagccg
     2821 ttgcctatac tccaggcagg aaactggagg aaatctcatg agcgaacccc tgttaattgc
     2881 ccgcacgccg gacacagaac tgtttttact gccgggaatg gctaaccgtc acgggctgat
     2941 tactggcgca acggggacgg gtaaaaccgt tacgctgcaa aaactggcag agtcattgtc
     3001 ggaaatcggc gtgccggtgt ttatggctga tgtgaaaggc gatctgaccg gtatcgcgca
     3061 ggcaggaacg gcgtcggaaa aactgctcac aaggcttaaa aatatcggcg tcaatgactg
     3121 gcaaccgcat gccaatccgg tggtggtgtg ggatatcttt ggcgagaaag gccacacggt
     3181 gcgggcgacg gtttcggatc tggggccgct gttgctggcg cgactgttga atctcaacga
     3241 tgtgcaatct ggcgtgctga atattatctt ccgcattgcg gacgatcagg gattgttgct
     3301 gctcgacttt aaagatttgc gggcgattac ccagtacatc ggcgataacg ccaaatcctt
     3361 ccagaatcag tacggaaata tcagtagcgc atcggttggt gccatccagc gcggattact
     3421 gtcgctggaa cagcaaggcg cagcacactt ctttggtgag ccgatgctgg atatcaaaga
     3481 ctggatgcgc accgatgcca acggtaaagg cgttatcaat atcctcagcg ccgagaaact
     3541 ttatcagatg ccgaaactgt acgccgccag cctgctgtgg atgctttcag agttgtatga
     3601 acaattgccg gaagcgggcg atctggagaa accaaaactg gtgtttttct tcgacgaggc
     3661 acatctgctg tttaacgatg caccgcaggt actgctggat aagattgagc aggtgataag
     3721 gcttattcgc tcaaaaggcg tgggcgtctg gttcgtttcg caaaacccgt ctgatattcc
     3781 ggataatgtg ctcggccagc tcggtaatcg tgttcagcac gctttgcggg cttttacgcc
     3841 aaaagatcag aaagcagtga agacagcggc gcaaaccatg cgggccaatc cggcatttga
     3901 taccgaaaag gcaatccagg aactggggac cggcgaggcg ttaatctcgt ttctcgatgc
     3961 aaaaggaagt ccttctgtgg tggaacgggc gatggtgatc gcaccttgtt cgcgaatggg
     4021 gccggtgacg gaagatgagc gtaatggcct gattaatcac tccccggtgt atggcaaata
     4081 tgaagatgag gtggaccgcg agtccgccta tgagatgctg caaaaaggct ttcaggccag
     4141 taccgagcag caaaataatc cccccgtgaa agggaaagag gtggcggtgg atgacggtat
     4201 tcttggtgga ttgaaggata ttttgtttgg cactaccgga ccacgcggcg ggaagaaaga
     4261 tggtgtggtg caaacaatgg cgaaaagcgc cgctcgccag gtgacgaatc agattgtacg
     4321 cgggatgttg gggagtttgc tgggggggga gaaaaagata agtcagacgt gatctgtgcc
     4381 cctcacccta accctctccc caaaggggcg aggggaccgt tcgaactcgt atttgacttt
     4441 gtcaacaata ataaagggag ctttcgctcc cttgttagat tacgattttc tcattaacag
     4501 ccacaggctg attaagaaga agctggcact tggcaacagt gcgccgatga tcggcgggat
     4561 gccgtaaacc aacgtcagcg ggccgaagat ctggtccagt acgtagaaga caaaaccgaa
     4621 gctgatacct gtgaccacac gcacgcccat cggtacgcta cgcagtgggc caaagataaa
     4681 cgacagcgcc atcagcatca tcaccgccac ggatagcggc tggaagattt tgctccacat
     4741 gttgagctga taacgtccgg catcctgacc gctcgacttc agatacttca cgtagttgtg
     4801 caaaccactg atggagagtg catccgggtc cagcgccacc acgcccagtt tgtctggcgt
     4861 gaggtcggtt ttccaggtgc cgctcaccgt ctgcgaaccg gtaatctgtt tcggattggt
     4921 cagatcagat tcatcaacct gcgacagacg ccagacttta tgttccgggt caaacttcgc
     4981 ggtagcggca taacgtacgg attgcagacg acgattctcg ttaaaggcat aaatactgat
     5041 gccacctaac acctcgtcat ctttaacccg ctcaatgtag acgaagttgt tgccatcttt
     5101 cgcccataaa ccttgctggg tagagagcaa cgagccgccg tacatcgcct gcgcacggta
     5161 gttacgcgcc atctgttcgc cctgcggcgc gacccattcg ccaatcgcca tcgtcagcaa
     5221 gaccagtgga atggcggttt tcatcaccga cagcgctacc tgcatacggg taaaaccaga
     5281 agcctgcatc accaccagtt cgctgcgctg cgccagcatc ccaagaccaa gcaacgcccc
     5341 aagaagagcc gccatcggga agaagatctg cacatctttc ggcacgctca gcaaggtata
     5401 cattcctgcg cctaacgcgt cgtaactccc ctgcccggct tttttcagct gatcgacaaa
     5461 tttgataatg cccgacagcg acaccagaat gaacagcgtc atcatgatgg tggtgaaaat
     5521 agttttaccg atatagcggt caagtacgcc aaaaggttgc atcacaccgc tcctttacgc
     5581 gaaaaactgg cgcgcaggcg gcggaccggc acggtgtccc aaaggttgag aacaatcgcc
     5641 aaagccagat aaatcaggtt aacggtccac atccacagtg tcgggtccag cttaccttta
     5701 ccgccgttcg atttcaggga ggtctggatc aggaagaaaa gtagatacag cagcatggct
     5761 ggcagcatcg acagtacgcg tccctgacgc gggttaacca cgctcaacgg tacaaccata
     5821 agtgccatca taaacacggt gaataccaac gtgatacgcc agttcagttc tgcgcgagca
     5881 cgatcggtgt cagtgttcca caatgtgcgc atgtccatct ggtcggtatc gttcgggtcg
     5941 agcgccaccg cctggtgacc aatgatcgcc tgataatcct ggaaatccgt aatgcggaaa
     6001 tcacgtaaca gtgcagtgcc ttcgaagcgc gttccctggt tgagagtgac gacctgggag
     6061 ccgtcgcgca gctgggttaa atgtccggaa tcggccacca ccacagaagg acgtgcatta
     6121 ccttttggtc gaatttgtgc gaggaacaca tctttgaaat cgctgccgtc aacgctttcg
     6181 atgaacagca ctgagctgcc attagtcgct tgctggaatt gcccttgcgc cagcgccgcc
     6241 atgccagggt tcgctttcgc ttctgctaac acttcatcct gatgacgcga tgaccacggt
     6301 cccgcccaca tcacgttaac cgccgcgacg attgccgtga ataccgcaag gatcattgcc
     6361 gctttcacca gaaccgcttt gctcaagccg caggcatgca ttaccgtaat ttcactttcg
     6421 gtatacagtt tgcccagcgt catcagcagc ccgaggaaca ggcttaatgg caggataagc
     6481 tgcgccattt ccggcacgcc caacccgaga agggagagca ccagattcgc cggaatatcg
     6541 ccgtcaaccg ctgcgccgag gatcctcact aacttttgac agaagaagat caaaagcaag
     6601 atgaagagta tcgccagctg gcttttgagc gtctcccgca ccagatatct tatgattatc
     6661 actttaaata cgcccgtaaa aactcgtctt ttgcaggatt ttagcttgtt tcatggctta
     6721 aacgtcattt attctcttga gtcgtcgaaa tcgtcgctaa gataattata ctcaacggat
     6781 tcacctctca gattttgttc tgacgtgcca atgccgtaat aacgttaaga ttaacacgaa
     6841 gtcatcgcaa cagcggacat gagttacgaa agcttgcaat tctatctgta gccaccgccg
     6901 ttgtctttaa gattcaggag cgtagtgcat ggagtttagt gtaaaaagcg gtagcccgga
     6961 gaaacagcgg agtgcctgca tcgtcgtggg cgtcttcgaa ccacgtcgcc tttctccgat
     7021 tgcagaacag ctcgataaaa tcagcgatgg gtacatcagc gccctgctac gccggggcga
     7081 actggaagga aaaccggggc agacattgtt gctgcaccat gttccgaatg tactttccga
     7141 gcgaattctc cttattggtt gcggcaaaga acgtgagctg gatgagcgtc agtacaagca
     7201 ggttattcag aaaaccatta atacgctgaa tgatactggc tcgatggaag cggtctgctt
     7261 tctgactgaa ctgcacgtta aaggccgtaa caactactgg aaagtgcgtc aggctgtcga
     7321 gacagcaaaa gagacgctct acagtttcga tcagttgaaa acgaataaga gcgaaccgcg
     7381 tcgtccgctg cgtaaaatgg tgttcaacgt gccgacccgc cgtgaactga ccagcggtga
     7441 gcgcgcgatc cagcacggtc tggcgattgc cgccgggata aaagcagcga aagatctcgg
     7501 taatatgccg ccgaatatct gtaacgccgc ttatctcgcc tctcaggcgc gccagctggc
     7561 tgacagctac agcaagaatg tcatcacccg cgttatcggc gaacagcaga tgaaagagct
     7621 gggaatgcac tcttatctgg cggtcggtca gggttcacaa aacgaatcgc tgatgtcggt
     7681 gattgagtac aaaggcaacg cgtcggaaga tgcacgccca atcgtgctgg tgggtaaagg
     7741 tttaaccttc gactccggcg gtatctccat caagccttca gaaggcatgg atgagatgaa
     7801 gtacgatatg tgcggcgcgg cggcggttta cggcgtgatg cgtatggtcg cggagctgca
     7861 actgccgatt aacgttattg gcgtgctggc aggctgtgaa aacatgcctg gcgggcgtgc
     7921 ctatcgtccg ggcgatgtgt taaccaccat gtccggtcaa accgttgaag tgctgaacac
     7981 cgatgccgaa ggccgcctgg tactgtgcga cgtgttaact tacgttgaac gttttgagcc
     8041 ggaagcagtg attgacgtgg cgacgctgac cggtgcctgc gtgatcgcgc tgggtcatca
     8101 tattactggt ctgatggcga accataatcc gctggcccat gaactgattg ccgcgtctga
     8161 acaatccggt gaccgcgcat ggcgcttacc gctgggtgac gagtatcagg aacagctgga
     8221 gtccaatttt gccgatatgg cgaacattgg cggtcgtcct ggtggggcga ttaccgcagg
     8281 ttgcttcctg tcacgcttta cccgtaagta taactgggcg cacctggata ttgcaggaac
     8341 cgcctggcgt tctggtaaag caaaaggcgc aaccggtcgt ccggtagcgt tgctggcaca
     8401 gttcctgtta aaccgcgctg ggtttaacgg cgaagagtaa gtttgcgttt taccctcacc
     8461 ctaaccctct ccctaaggga gagggaaccg atcgagcgca atagaaattc attgctcatc
     8521 tttcagggtg aggaatgcag caatatttaa atccaccaca agaagcccca tatatgaaaa
     8581 acgcaacgtt ctaccttctg gacaatgaca ccaccgtcga tggcttaagc gccgttgagc
     8641 aactggtgtg tgaaattgcc gcagaacgtt ggcgcagcgg taagcgcgta ctcatcgcct
     8701 gtgaagatga aaagcaggct taccggctgg atgaagccct gtgggcgcgt ccggcagaaa
     8761 gctttgttcc gcataattta gcgggagaag gaccgcgcgg cggtgcgccg gtggagatcg
     8821 cctggccgca aaaacgcagc agcagcccgc gcgatatatt gattagtctg cgaacaagct
     8881 ttgcagattt tgccaccgct ttcacagaag tggtagactt cgttccttat gaagattctc
     8941 tgaaacaact ggcgcgcgaa cgctataaag cctaccgcgt ggctggtttc aacctgaata
     9001 cggcaacctg gaaataatgg aaaagacata caacccacaa gatatcgaac agccgcttta
     9061 cgagcactgg gaaaagcagg gctactttaa gcctaatggc gatgaaagcc aggaaagttt
     9121 ctgcatcatg atcccgccgc cgaacgtcac cggcagtttg catatgggtc acgccttcca
     9181 gcaaaccatc atggatacca tgatccgcta tcagcgcatg cagggcaaaa acaccctgtg
     9241 gcaggtcggt actgaccacg ccgggatcgc tacccagatg gtcgttgagc gcaagattgc
     9301 cgcagaagaa ggtaaaaccc gtcacgacta cggccgcgaa gctttcatcg acaaaatctg
     9361 ggaatggaaa gcggaatctg gcggcaccat tacccgtcag atgcgccgtc tcggcaactc
     9421 cgtcgactgg gagcgtgaac gcttcaccat ggacgaaggc ctgtccaatg cggtgaaaga
     9481 agttttcgtt cgtctgtata aagaagacct gatttaccgt ggcaagcgcc tggtaaactg
     9541 ggacccgaaa ctgcgtaccg ctatttctga cctggaagtg gaaaaccgcg aatcgaaagg
     9601 ttcgatgtgg cacatccgct atccgctggc tgacggcgcg aaaaccgcag acggtaaaga
     9661 ttatctggtg gttgcgacca ctcgcccgga aaccctgctg ggcgataccg gtgtagctgt
     9721 taacccggaa gatccgcgtt acaaagatct gattggcaaa tatgtcattc tgccgctggt
     9781 taaccgtcgt attccgatcg ttggcgacga acacgccgac atggaaaaag gcaccggctg
     9841 cgtgaagatc accccggcgc acgactttaa cgactatgaa gtcggtaaac gtcacgccct
     9901 gccgatgatc aacatcctga cctttgacgg tgatatccgt gaaagcgccc aggtgttcga
     9961 taccaaaggt aacgaatctg acgtttattc cagcgagatc ccggcagagt tccagaaact
    10021 cgagcgtttt gctgcacgta aagcagtcgt tgctgcggtt gatgcactcg gcctgctgga
    10081 agaaattaaa ccgcacgacc tgaccgttcc ttacggcgac cgtggcggcg tggttatcga
    10141 accgatgctg accgaccagt ggtacgtgcg tgccgatgta ctggcgaaac cggcagttga
    10201 agcggttgag aacggcgaca ttcagttcgt accgaagcag tacgaaaaca tgtacttctc
    10261 ctggatgcgc gatattcagg actggtgtat ctctcgtcag ctgtggtggg gtcaccgtat
    10321 cccggcatgg tatgacgaag cgggtaatgt ttatgttggc cgcaacgaag aagaagtgcg
    10381 caaggaaaat aacctcggtg ccaacgttgc cctgcgtcag gacgaagacg ttctcgacac
    10441 ctggttctct tctgcactgt ggaccttctc tacccttggc tggccggaaa ataccgacgc
    10501 cctgcgtcag ttccacccaa ccagcgtgat ggtatctggt ttcgacatca tcttcttctg
    10561 gattgcccgc atgatcatga tgaccatgca cttcatcaaa gatgaaaatg gcaaaccgca
    10621 ggtgccgttc cacactgttt acatgaccgg tctgattcgt gacgacgaag gtcagaagat
    10681 gtccaaatcc aagggtaacg ttatcgaccc gctggatatg gttgacggta tttcgctgcc
    10741 agaactgctg gaaaaacgca ccggcaatat gatgcagccg cagctggcgg acaaaatccg
    10801 taagcgcacc gagaaacagt tcccgaacgg cattgagccg cacggcaccg atgccctgcg
    10861 cttcaccctg gcggcgctgg cgtctaccgg tcgtgacatc aactgggata tgaagcgtct
    10921 ggaaggctac cgtaacttct gtaacaagct gtggaacgcc agccgctttg tgctgatgaa
    10981 cacagaaggt caggattgcg gcttcaacgg cggcgaaatg acgctatcgc tggcggaccg
    11041 ctggattctg gcggagttca accagactat caaagcgtac cgcgaagcgc tggacagctt
    11101 ccgcttcgat atcgccgcag gcattttgta tgagttcacc tggaaccagt tctgcgactg
    11161 gtatctggag ctgaccaagc cggtaatgaa cggtggaaca gaagccgaac tgcgcggtac
    11221 tcgccatacg ctggtgactg tactggaagg tctgctgcgc ctcgcgcatc cgatcattcc
    11281 gttcatcacc gaaaccatct ggcagcgtgt gaaagtactt tgcggtatca ctgccgacac
    11341 catcatgctg cagccgttcc cgcagtatga tgcatctcag gttgatgaag ccgcactggc
    11401 cgacaccgag tggctgaaac aggcgatcgt tgctgtacgt aacatccgtg cagaaatgaa
    11461 catcgcgccg ggcaaaccgc tggagctgct gctgcgtggt tgcagcgcgg atgcagaacg
    11521 tcgcgtaaat gaaaaccgtg gcttcctgca aacgctggcg cgtctggaaa gcatcaccgt
    11581 gctgcctgcc gatgacaaag gtccggtttc cgttaccaag atcatcgacg gcgcagagct
    11641 gctgatcccg atggctggcc tcatcaacaa agaagatgag ctggcgcgcc tggcgaaaga
    11701 agtggcgaag atcgaaggtg aaatcagccg catcgagaac aagctggcaa atgaaggctt
    11761 tgtcgcccgc gcaccggaag cggttatcgc gaaagagcgt gagaagctgg aaggttatgc
    11821 agaagcgaaa gccaagctga ttgaacagca ggctgttatc gccgcactgt aatcaacaca
    11881 gtgatgaaaa cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa
    11941 agaaggcata actccccgag aaatactggc taagaagcct ttatctggtt gttcttcttt
    12001 attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc
    12061 aatcatccat attttcagca gtgggtaagc cagaccaccc gtaatctcgg atatcaccac
    12121 caacgcacac atgcgataaa gcataccgtg gtacgttaaa gttgagcgaa aacgaatacg
    12181 cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact
    12241 ggtgctaaca gcaatcccaa aataataaat taactgcgcg attatcattt tacg
//
LOCUS       DADMEQ010000113        12230 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.114, whole genome shotgun sequence.
ACCESSION   DADMEQ010000113 DADMEQ010000000
VERSION     DADMEQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12230
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.114"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..494)
                     /locus_tag="JNZ05_003197"
     CDS             complement(<1..494)
                     /locus_tag="JNZ05_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566324.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(525..1217)
                     /gene="fimC"
                     /locus_tag="JNZ05_003198"
     CDS             complement(525..1217)
                     /gene="fimC"
                     /locus_tag="JNZ05_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAY8566325.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1418..1960)
                     /gene="fimA"
                     /locus_tag="JNZ05_003199"
     CDS             complement(1418..1960)
                     /gene="fimA"
                     /locus_tag="JNZ05_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein subunit FimA"
                     /protein_id="HAY8566326.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2441..3307
                     /gene="folD"
                     /locus_tag="JNZ05_003200"
     CDS             2441..3307
                     /gene="folD"
                     /locus_tag="JNZ05_003200"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HAY8566327.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3309..3521
                     /gene="ybcJ"
                     /locus_tag="JNZ05_003201"
     CDS             3309..3521
                     /gene="ybcJ"
                     /locus_tag="JNZ05_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HAY8566328.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3629..4150
                     /locus_tag="JNZ05_003202"
     CDS             3629..4150
                     /locus_tag="JNZ05_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY8566329.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(4186..5571)
                     /gene="cysS"
                     /locus_tag="JNZ05_003203"
     CDS             complement(4186..5571)
                     /gene="cysS"
                     /locus_tag="JNZ05_003203"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAY8566330.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5745..6239
                     /gene="ppiB"
                     /locus_tag="JNZ05_003204"
     CDS             5745..6239
                     /gene="ppiB"
                     /locus_tag="JNZ05_003204"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HAY8566331.1"
                     /translation="MVTFHTNHGDIVIKTFDNKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6242..6964
                     /gene="lpxH"
                     /locus_tag="JNZ05_003205"
     CDS             6242..6964
                     /gene="lpxH"
                     /locus_tag="JNZ05_003205"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HAY8566332.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7082..7591
                     /gene="purE"
                     /locus_tag="JNZ05_003206"
     CDS             7082..7591
                     /gene="purE"
                     /locus_tag="JNZ05_003206"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAY8566333.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7588..8655
                     /gene="purK"
                     /locus_tag="JNZ05_003207"
     CDS             7588..8655
                     /gene="purK"
                     /locus_tag="JNZ05_003207"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAY8566334.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8792..9685)
                     /locus_tag="JNZ05_003208"
     CDS             complement(8792..9685)
                     /locus_tag="JNZ05_003208"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAY8566335.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9682..10329)
                     /locus_tag="JNZ05_003209"
                     /pseudo
     CDS             complement(9682..10329)
                     /locus_tag="JNZ05_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10332..11564
                     /gene="allC"
                     /locus_tag="JNZ05_003210"
     CDS             10332..11564
                     /gene="allC"
                     /locus_tag="JNZ05_003210"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HAY8566336.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11575..>12230
                     /gene="allE"
                     /locus_tag="JNZ05_003211"
     CDS             11575..>12230
                     /gene="allE"
                     /locus_tag="JNZ05_003211"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HAY8566337.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPAEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     "
BASE COUNT         2967 a         3170 c         3118 g         2975 t
ORIGIN      
        1 tgagcgctac tcaacaacgc tatctgagga atagtgatgt tcaggcgcat cgctgcaaaa
       61 tcaaagttaa ttgtggcatc aggtacagct ttcagtaaat tgatgcattt attgttttgc
      121 tgctgggcta actcgggaaa tgcactgcta ttaaggccaa ttcgttcaag gagtacctgg
      181 ttaaaacagg gcattaaccc acctgattta tcacctgtat tttctgttgt cgattcaaat
      241 acgatatcct gtgaaccaat gaactcatca ttacgccaga gatccacccg ataaacgccc
      301 gcaggttgat gattacctct ctcaaagcgc gataaatcag caacggatgt gccattctct
      361 aataaaaatg ccgggttgaa ataactttcc gcatagctta aaaaggaggg taaaatggca
      421 agagatgaat aacaaattcc ggccagacac caggtatacc tctgcggaat atccgtagta
      481 gtgggtattt tcataattag ctctgatatt gtcagatgat tcggttagtt taagttcact
      541 tctctgaccg gggttactga accataatca ttaacggtct gaaatttcag ctttccctgc
      601 actcctgagg gtaagggaat ttgtgaacta actctgggtg ccgccatagc attccccaac
      661 ttttggctgc ccacttgcag gttaacgagg gtgacataaa aaggtgatgg attatgaaca
      721 ttgatatagt taccggatcg cgaaaacttg agtgtgtccg gcgcttctgc gggtaattct
      781 tgtaattgaa ttggacggag aaataatttc atgcgcgata aaatcgccag ttgcaaaaca
      841 ttcctgccgt tcaatgcatt tttatctacc gaagggatcg tcttaacatt catccagaac
      901 agagactcac gatctgccgc cagcggtgga ccggtgtaaa taatacgcaa agtattttcg
      961 cttttgggtt cactaacaaa tagtggcggt gtaatgatga atgacttttc ttttacaccg
     1021 ctgctgtttt caatccacga attgaccaga aagagctcat tggtatggct atttttaatc
     1081 cataccgcag tctgtttagc atcagcggga taaataatac gcgtggcacc taatgcgatc
     1141 cctccggcag catgggcgct ggccgaaatg attaaataaa atatcatgag catcaataac
     1201 tttattttag tcatcatagt taatacgcct tgtgtattcc tgacggcaaa agattgcttt
     1261 ggcttcttac aacacatcat tcccttttca tttaataaag cggcatgata caaagtgaac
     1321 gtggtttcga tttacattgc caccataccg cttcagacaa ccggtacatt actttgttat
     1381 aagacgtgat atataattca aaacaacgtg gttttgatta ttcgtatctc ataataaaag
     1441 tcgcgtcagc atttgcttgc cctgctgatg cacttgtacc ggtgccttta taacgtgcag
     1501 aaaaatgaag aacgttggtc ccggggatca agttctggtt ggttgagaag ctattcccat
     1561 caggtttgag aattgcggat gtattatcaa gtatttcaat accgacaccc gttgctgttg
     1621 ttgtatttgt actggatgca atggccaata aattatcatt gattgcatca gcctgaccag
     1681 aaaatgcaac ggcagcatta gcggcaacaa caggatcgca gtcgttcaac tgaatggtga
     1741 atggaattaa tgcagaggta ttaccaacag cattgaaaat atcggtacga tattgaccga
     1801 gtgtgacaac ctggtctgcc gagtcagtat tcactgaaca ggcagcattc accagttcgc
     1861 cttcaaagtg aattgtaccg ccatctacaa cggcagcata actacccgta gcggcgaata
     1921 gtgcggcagc cagcgcagac gaaataaatc ttaatttcat atatattcct tcaatctcat
     1981 ttatcgactc cacatccgta tataaccgat tactttattt aagacactga tagtagtaaa
     2041 attccttttt atcctctaag aatgtcttaa ttgaaaatat gcactctatt ctaaaaaata
     2101 gagagccccg ttagatgaat acttccgcgc aaaatatatt caacacaaat atagacctga
     2161 agcggtaaat taccaggctg aaaattcttt ttatattgtc aggtatttct taaattatct
     2221 taatccttaa acaaggaaat aaatcagttc cagatttaca acgccatcat gtacgaaaaa
     2281 tgaagctttc aatctcagcg acctcagcga cggtgcgcct aaccttcgca agaggtcgct
     2341 tcacgcgata aatctgaaac gaaacctgac agcgcgcccc gcttctgaca aaatagacgc
     2401 atccccttcg acctacgtaa cagatggaat cctctctctg atggcagcaa agattattga
     2461 cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct caaaaagttc aggctcgtat
     2521 tgcagccgga ctgcgggcac caggactggc cgttgtgctg gtgggtagta accctgcatc
     2581 gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa gtcgggttcg tctcccgctc
     2641 ttatgacctc ccggaaacca ccagcgaagc ggagctgctg gagcttatcg atgcgctgaa
     2701 tgccgacaac accatcgatg gcattctggt tcaactgccg ttaccggcgg gtattgataa
     2761 cgtcaaagtg ctggaacgta ttcatccgga caaagacgtg gacggtttcc atccttacaa
     2821 cgtcggtcgt ctgtgccagc gcgcgccgcg tctgcgtccc tgcaccccgc gcggtatcgt
     2881 cacgctgctt gagcgttaca acattgatac cttcggcctc aacgccgtgg tgattggcgc
     2941 atcgaatatc gtcggtcgcc caatgagcat ggaactgttg ctcgcaggtt gcaccaccac
     3001 cgtgactcac cgcttcacta aaaatctgcg tcatcacgta gaaaatgccg atctgttgat
     3061 cgttgccgtt ggcaagccag gctttattcc cggtgactgg atcaaagaag gcgcaattgt
     3121 gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt gtgggcgacg tcgtgtttga
     3181 agacgcggct aaacgcgcct catacattac gcctgttccc ggcggcgttg gcccgatgac
     3241 ggttgccacg ctgattgaaa acacgctaca ggcgtgcgtt gaatatcatg atccacaggg
     3301 tgagtaacat ggcgacattt tctttaggta aacatccgca cgttgagctg tgcgacttgc
     3361 tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa aatcgcgatt gccgaaggcc
     3421 aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg caaaatcgtc gccggtcaga
     3481 cagtgagttt tgcaggacac agcgtacagg ttgttgcctg atccctttcc cggcctgtat
     3541 caacaggccg gttctctttt cagacttttg ccctctacgc cccgttcatc catgctgata
     3601 gcatcgacat tttgccgaag cgaccatcat gccaaccgtt attacgcacg ccgccgttcc
     3661 cctttgcatt ggtttagggt tgggttcgaa agtcatccct ccgcgtctgt tatttgccgg
     3721 aatcatccta gcgatgctgc ccgatgccga cgtattgtcg tttaaatttg gcgttgctta
     3781 cggcaatgtt tttggtcatc gcgggtttac ccattcgctg gtgtttgcgt ttgttgtccc
     3841 gctcttatgt gtgctgattg gacgtcgatg gttcagggct gggctgattc gctgctggct
     3901 gtttttaacc gtctcgttgc tatcgcacag cttactggat tcggtaacca ctggcggtaa
     3961 gggcgttggc tggctgtggc cgtggtcaga tgaacgcttt ttcgctccct ggcaggtgat
     4021 taaagtcgcg ccgtttgcat tgtctcgtta caccacagcg tacgggcatc aggtgattat
     4081 ttccgaattg atgtgggtgt ggttgccggg gatgctactg atgggaatgt tgtggtggcg
     4141 cagacgataa ccggatgcga aaactcgcat ccggcaattg cgcaattact tacgacgcca
     4201 ggtggtccct tgcgggccat cttccagcac gatccccatc tcgttaagac ggtcacgtgc
     4261 cgcatctgcc gccgcccagt ctttcgcttt acgggcatcc agacgctgtt gaattaacgc
     4321 ttcaatctca gccacttcgc tgtcgtctgc ctgcgcgccg ctttgcagga acgcttccgg
     4381 ttcttgctcc agcaggccca atacagcgga aagtttacgc aggtgagatg ccattgcatt
     4441 cgccgctgcc atatcttctg ctttcagacg gttcacttca cgcgccatat caaacagcac
     4501 ggaataggct tccggggtgt tgaaatcgtc gtccatcgct tcaataaagc gcgcttcaaa
     4561 cgcttcgcca ccggcaggcg caacggtttt atctgtgccg cgcagcgcag tgtagaggcg
     4621 ctccagcgcc gcacgcgcct gcttcaggtt ctcttcgctg tagttcagct ggctgcgata
     4681 gtggcccgac atcaggaagt aacgcacggt ttccgcgtcg tagtatttca gcacatcgcg
     4741 cacggtaaag aagttaccca gcgatttgga catcttctcg cggtcaacca tcaccattcc
     4801 ggagtgcatc cagtagttca catactgacc atcatgggca caggtggact gcgcgatttc
     4861 gttctcgtgg tgcgggaaca tcaggtctga accgccgccg tggatatcaa agtggttacc
     4921 cagctgcttg cagttcatgg ccgaacattc aatgtgccag ccaggacgac ccgcgcccca
     4981 cggagacggc cagctcggtt cgccctcttt cgacatcttc cacagaacga agtccatcgg
     5041 gttgcgtttg tcatcaacca cgtcaacgcg cgcaccagcc tgcagctggt cgagatcctg
     5101 acgcgacagc acgccataag ttggatcggt cgggacgtcg aacatcacgt cgccgttgtc
     5161 cgccacataa gcgtgacctt tggcgatcag ttgttcagtg agttcaataa tttctgcgat
     5221 atggtgcgtc gcgcgcggct ccatatccgg gcgcagaatg ttcaaagcat caaaatcttt
     5281 gtgcatttcg gcgatcatgc gatccaccag cgccacaaag ctttcgccat tttcattggc
     5341 gcgtttgatg attttgtcgt cgatatcggt aatgttgcgc acatacttca gtttatagcc
     5401 gaggaaacgc agatagcgcg caaccacgtc aaaagcaaca aaggtacgcc cgtgaccgat
     5461 atgacagaga tcgtaaacgg tgattccaca cacgtacatg ccgacttccc cggcgtgaat
     5521 aggcttaaat tcctcttttt ggcgtgtcag agtattgaag atttttagca tcgaagattc
     5581 cgtttagaca tgtgtgggta attgagttgc gtataatacc catatttccc gcgcgaatca
     5641 gcatacattg cgtgatgatc cgatcgtgcg gttatgctat aacaccaccc tatatatgac
     5701 ccgaactggg ttgaagcacc aatcaaacgg aacaggatgc aaaaatggtt actttccaca
     5761 ccaatcacgg cgatattgtc atcaaaactt ttgacaataa agcacctgaa acagttaaaa
     5821 acttcctgga ctactgccgc gaaggttttt acaacaacac cattttccac cgtgttatca
     5881 acggctttat gattcagggc ggcggttttg aaccgggcat gaaacaaaaa gccaccaaag
     5941 aaccgatcaa aaacgaagcc aacaacggcc tgaaaaatac ccgtggtacg ctggcaatgg
     6001 cacgtactca ggctccgcac tctgcaactg cacagttctt catcaacgtg gttgataacg
     6061 acttcctgaa cttctctggc gaaagcctgc aaggttgggg ctactgcgtg tttgctgaag
     6121 tggttgacgg catggacgtg gtagacaaaa tcaaaggtgt tgcaaccggt cgtaacggta
     6181 tgcaccaaga cgtgccaaaa gaagacgtta tcattgaaag cgtgaccgtt agcgagtaat
     6241 cgtggcgaca ctctttattg cagatcttca tctctgcgtg gaagaaccgg cgatcaccgc
     6301 cggttttctg cgttttttag cgggggaagc ccgcaaggcc gacgcgctgt atattcttgg
     6361 cgatctgttt gaagcatgga ttggcgacga cgatcccaac ccactccatc gccagatggc
     6421 ggcagcgatc aaagcggtgt ccgattccgg cgttccctgt tatttcattc atggcaaccg
     6481 tgattttctg ctcggcaaac gctttgcccg tgaaagcggc atgacattat tgccggaaga
     6541 aaaggtgctc gaactttatg gtcgccgggt gttgattatg catggcgaca cgctgtgcac
     6601 cgatgacgcg ggttatcagg cttttcgcgc caaagtccac aaaccctggc tgcaaatgct
     6661 attcctcgcc ctgccgttgt ttgtgcgcaa acgcattgcc gcgcgaatgc gcgcgaacag
     6721 caaagaagcc aacagcagta aatcgctggc gatcatggac gttaaccaaa acgctgtggt
     6781 cagtgcgatg gaaaaacatc aggtgcaatg gctaatccac gggcataccc atcgcccggc
     6841 ggtgcatgaa cttatcgcca accagcaacc tgcttttcgc gtggtactgg gtgcctggca
     6901 tacggaaggt tcgatggtga aagtcacggc ggatgacgtt gagctgattc attttccgtt
     6961 ttaaaaaacc cacaactttg ctgatttcac agccacgcaa ccgttttcct tgctctcttt
     7021 ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc accacaggag ttttaagacg
     7081 catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg atggggtcca aaagcgactg
     7141 ggctaccatg cagttcgccg tcgaaatctt cgaaatcctg aatgtcccgc accacgttga
     7201 agtggtttct gctcaccgca cccccgataa actgttcagc ttcgccgaaa gcgccgaaga
     7261 gaacggttat caggtgatta ttgcgggcgc aggcggcgca gcgcacctgc caggcatgat
     7321 tgccgccaaa acgctggtgc cggtgctggg cgtgccagta cagagcgccg cactgagcgg
     7381 tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc attccggtgg gtacgctggc
     7441 gattggtaaa gctggcgcgg caaacgcggc attactggca gcacaaattc tcgcgactca
     7501 cgataaagag ctacaccagc gtctgaatgg ctggcgcaaa gcccagaccg acgaagtgct
     7561 ggaaaacccg gacccgcgag gtgcggcatg aaacaggttt gcgtcctcgg taacgggcag
     7621 ttaggccgta tgctgcgtca ggcaggcgaa ccgttaggca ttgctgtctg gccggtcggg
     7681 ctggacgctg aaccggcggc ggtgcctttt caacaaagcg tgattaccgc tgagatcgaa
     7741 cgctggccgg aaaccgcatt aacccgcgag ctggcgcgtc atccggcctt tgtgaaccgc
     7801 gatgtgttcc cgattattgc tgaccgtctg actcagaaac agcttttcga taagctccac
     7861 ctgccgaccg cgccgtggca gttacttgcc gatcgcagcg agtggcctgc ggtgtttgat
     7921 cgtttaggtg aactggcgat tgttaagcgt cgcactggtg gctatgacgg tcgcggtcaa
     7981 tggagtttac gcgccaatga aaccgaacag ttaccggcag agtgttacgg tgaatgtatt
     8041 gtcgagcagg gcattaactt ctctggtgaa gtatcgctgg ttggcgcgcg cggctttgat
     8101 ggcagcaccg tgttttatcc gctgacgcat aacctgcatc aggacggtat tttgcgcacc
     8161 agcgtcgctt ttccgcaggc caacgcacag cagcaggcgc aagccgaaga gatgctgtcg
     8221 gcgattatgc aggagctggg ctatgtgggc gtgatggcga tggagtgttt tgtcaccccg
     8281 caaggtctgc tgatcaacga actggctccg cgtgtgcata acagcggtca ctggacacaa
     8341 aacggtgcca gcatcagcca gtttgagctg catctgcggg cgattaccga tctgccgtta
     8401 ccgcaaccag tagtgaataa tccgtcggtg atgatcaatc tgattggtag cgatgtgaat
     8461 tatgactggc tgaaattgcc gctggtgcat ctgcactggt acgacaaaga agtccgtccg
     8521 gggcgtaaag tggggcatct gaatttgacc gacagcgaca catcgcgtct gactgcgacg
     8581 ctggaagcct taatcccgct gctgccgcca gagtatgcca gcggcgtgat ttgggcgcag
     8641 agtaagttca gttaagcagg tgctcatttg attgccggat gcggcgtaaa cgccttatcc
     8701 ggcttaccgc tccggtaccc attgtaggcc tgataagacg cgtcaagcgt cgcatcaggc
     8761 atggtgcacc gattgccgga tgctctcatc actacagcga aatacaggtc cccgcttcgc
     8821 ccgccagcgt ctcttcaatt cgcgataacg ccccaatcca cgcgggttta ctacggcttc
     8881 tgacataacc actcaccgcc gttaccttcg gccccatcga accatcggct ttggcaaatg
     8941 gcgctaactc atccggtgtg gcatggcgaa tggcacgttg ctgcggcgtt ccccagtttt
     9001 catataccgc atcagcatcg gtgaggatca ccagtccatc ggcattaatc tgctcggcga
     9061 gcaacgcagc ggcgagatct ttatcaatca ccgcttcact ccctgcccca tcttccgtca
     9121 caggcacacc gccgccgcca ctgcaaatca ccacatgccc ctctttgagc aacaactcga
     9181 tggcttcgct gtcgagaatt ttacgcggtt gcggagacgc caccacccgg cgcaaatatt
     9241 taccatcacg tttcatctgc cagccgtaag ccgcttccag tgcctcttgt tcttctggct
     9301 gataaaccgg accaataaat ttctctggct gcaaaaacgc cggatcatca ggcgaaacct
     9361 caatgcgcgt cagcaccgtc gtaacaggcg gcatctgcgg ctgtgcgctc aagctctgcg
     9421 ccagcatata gccaatcatc ccctggcttt ccgccaccag cacatccagc ggatacggtt
     9481 ctacctcttt ccacgccaga ttctgtaacg ccagcagccc cacctgcggc ccgttgccgt
     9541 gaacaatcgc cagccgataa gaacgggcca ggcgtgccag cgcgggtaca gcactggcga
     9601 tattgcgata ttgattttcc gccgtcagcg cctcaccgcg ctggagtaag gcgttgcccc
     9661 caagagctac aaccagtgtt ttcatggttt tccttgtaat aattgttggc caagccagaa
     9721 ccccagcagc gtatccgccc ctgacgtatg ccccagcgcc agcagcgaat caatcgcaac
     9781 agcggtacgt ttcgggcaac tcagagcatg gacaaagtgc aggagtggcg aggcgaaata
     9841 tccttgcgcg gcataacgta aataactgac gctcaccgct gtggtaacga gttgaagatt
     9901 gtcggaacag gcaaaaaacg ggcgaccggc gcgcgcatct aaagcgccat aataccaggc
     9961 cgccagcagc attccgctca acgtgtcatc atgacttggc gttaatccgg ggcctttacc
    10021 cagccagtgc cgccagtcgg tcttaacgcc attgagcgcg gcctgaaaac agtgacgaaa
    10081 ctggcgtaat tcagcgggca gcggatcgct tgccgccatc gccagtggcc cgaaaagtcc
    10141 ggtttcctcc gcgcgttgca tccatgcagc tgcaagcggt tgaggatgcg aaggcggcgt
    10201 aatacgcagc aaacaataac gctgtggctg tttaaccgtg aaacgcccga ggcgaatccc
    10261 ttgagccaca acctgtggtc gttcattgcc gcataatcca ccgcataacg catcgaattg
    10321 cgtgcggcga aattacacat tcccgtcaag ctatagaaga aacgctgccc tggctttcct
    10381 cttttggcgc tgacccaacg ggtgggatga cccgtttact ttattcgccg gaatggctgg
    10441 aaacccagca gcaatttaaa aaaagaatgg cagcaagtgg gctggaaaca cgtttcgatg
    10501 aagtggggaa tttatacggt cgcctgagtg gcaccgaata tccacaggaa gtggttctga
    10561 gcggttcgca tatcgatacc gtggttaacg gcggtaacct cgacgggcaa ttcggcgcgc
    10621 tggcggcgtg gctggcaatt gactggttga aaacgcaata cggcgcaccg ttacgtacgg
    10681 tcgaagtggt ggcgatggca gaagaagaag gcagccgctt cccgtatgtc ttctggggca
    10741 gtaaaaatat ctttgggctg gcgaatcctg acgacgtgcg gaatatctgt gatgccaaag
    10801 gaaatagttt tgccgatgcg atgaaggctt gcggatttac tctgcctaac gccccactaa
    10861 ctccgcgtca ggatataaaa gcctttgttg aactgcatat tgaacagggc tgtgtgctgg
    10921 aaagtaatgg gcaatcaatt ggcgtggtga atgcaattgt cgggcagcgt cgctatacgg
    10981 tgacgctgaa cggcgaatca aaccatgcag gcaccacgcc gatgggttat cgtcgtgata
    11041 cggtttacgc tttcagtcgc atttgccatc agtcgatcga aaaagcgaaa aagatgggcg
    11101 atccgctggt tctgaccttt ggcaaagtag agccgcgccc gaatacggta aatgtggtgc
    11161 cgggtaaaac cacgttcacc attgattgtc gtcataccga cgctgccgtg ctgcgcgatt
    11221 tcacccaaca gttagaaaac gacatgcggg cgatttgcga tgaaatggac attggtattg
    11281 atatcgattt atggatggac gaagaacccg tgccgatgaa taaggagctg gtcgccaccc
    11341 tgacagaatt gtgtgaaaga gaaaaactga attaccgggt gatgcacagt ggtgccgggc
    11401 acgacgcgca aattttcgcg cctcgcgtac caacctgcat gatttttatc cccagcatca
    11461 acgggatcag ccataacccg gcggaacgca ccaatattac tgaccttgcc gaaggggtca
    11521 aaacgttggc actcatgctt tatcaacttg cctggcagaa ataaggagtc ataaatggga
    11581 tatttaaata acgtcaccgg ttaccgcgaa gatttactgg ctaaccgtgc gattgttaaa
    11641 cacggtaatt tcgcactgtt aaccccggat ggtctggtaa aaaatattat tccgggcttt
    11701 gaaaattgtg acgcgacaat cctctccacg ccaaagctgg gtgcctcttt tgttgattat
    11761 ctggtcacac tgcatcaaaa cggtggcaac caacagggct tcggtggcga aggcattgaa
    11821 acgttcctgt atgtgatctc tggaaatatc actgccaaag ccgaaggcaa aacatttgcc
    11881 ttaagcgaag gtggctatct ttattgccca ccaggctcct taatgacgtt tgttaacgcc
    11941 caggccgaag acagccaaat ctttttatat aagcgccgct atgttccggc agaaggccat
    12001 gcaccgtggc tggtttctgg caatgccagc gaactggaac gcattcacta tgaaggtatg
    12061 gatgatgtga tcctgctgga ttttctgccc aaagagttag gttttgatat gaacatgcat
    12121 atcctctctt ttgcaccagg tgccagccac ggttatatcg aaacacacgt tcaggaacat
    12181 ggtgcctata ttctttccgg tcagggggtt tataacctcg acaataactg
//
LOCUS       DADMEQ010000114        12165 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.115, whole genome shotgun sequence.
ACCESSION   DADMEQ010000114 DADMEQ010000000
VERSION     DADMEQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12165
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.115"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            7..1821
                     /gene="bglF"
                     /locus_tag="JNZ05_003212"
     CDS             7..1821
                     /gene="bglF"
                     /locus_tag="JNZ05_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HAY8566338.1"
                     /translation="MHCATRLRFKLKDESKAQAEVLKKTPGIIMVVESGGQFQVVIGN
                     HVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVYVISGIFTPLIGLMAATGILKG
                     MLALALTFQWTTEQSGTYLILFSASDALFGFFPIILGYTAGKRFGGNPFTAMVIGGAL
                     VHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVIPIIFSAWLCSILERRLNAWLP
                     SAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAAGYLWLYQAVPAFAGAVMGGFW
                     QIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIMAQVGAALGVFLCERDAQKKVV
                     AGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGALGATIIGYAQTKVYSFGLPSIF
                     TFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLHFITSKRQPAQGAPQEKTPEVI
                     TPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIAILPSVGEVRSPVAGRIASLFA
                     TLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVGDKVNTGDRLISFDIPAIREA
                     GFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSIIR"
     gene            1837..3234
                     /locus_tag="JNZ05_003213"
     CDS             1837..3234
                     /locus_tag="JNZ05_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAY8566339.1"
                     /translation="MTKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGK
                     MEPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEA
                     GLAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTR
                     YQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLI
                     PEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHN
                     ITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSER
                     GWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQ
                     VNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFG
                     WYAEVIKTRGLSLKK"
     gene            3320..4936
                     /locus_tag="JNZ05_003214"
     CDS             3320..4936
                     /locus_tag="JNZ05_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HAY8566340.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            4963..6138
                     /locus_tag="JNZ05_003215"
     CDS             4963..6138
                     /locus_tag="JNZ05_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAY8566341.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVLGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            6140..6862
                     /locus_tag="JNZ05_003216"
     CDS             6140..6862
                     /locus_tag="JNZ05_003216"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY8566342.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(6924..7511)
                     /locus_tag="JNZ05_003217"
     CDS             complement(6924..7511)
                     /locus_tag="JNZ05_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566343.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGMKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(7560..8033)
                     /locus_tag="JNZ05_003218"
     CDS             complement(7560..8033)
                     /locus_tag="JNZ05_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HAY8566344.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            complement(8100..8765)
                     /gene="yieH"
                     /locus_tag="JNZ05_003219"
     CDS             complement(8100..8765)
                     /gene="yieH"
                     /locus_tag="JNZ05_003219"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HAY8566345.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            8929..10266
                     /locus_tag="JNZ05_003220"
     CDS             8929..10266
                     /locus_tag="JNZ05_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY8566346.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            complement(10320..10886)
                     /gene="yieF"
                     /locus_tag="JNZ05_003221"
     CDS             complement(10320..10886)
                     /gene="yieF"
                     /locus_tag="JNZ05_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent chromate oxidoreductase YieF"
                     /protein_id="HAY8566347.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(10908..11657)
                     /locus_tag="JNZ05_003222"
     CDS             complement(10908..11657)
                     /locus_tag="JNZ05_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566348.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            complement(11814..>12165)
                     /locus_tag="JNZ05_003223"
     CDS             complement(11814..>12165)
                     /locus_tag="JNZ05_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8566349.1"
                     /translation="FRKECPLNSGYLRALDNVLQELGRERTIAMSLPEFEQSLFMAAQ
                     PDNLLLATAPRYCQYYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIV
                     WLRETIKNLYASMA"
BASE COUNT         3043 a         2849 c         3085 g         3188 t
ORIGIN      
        1 agtctgatgc attgcgcaac gcgattacgt tttaagttaa aggatgaaag caaagcgcaa
       61 gctgaggtac tgaaaaagac ccccggtatt attatggtgg tggaaagtgg tggtcagttt
      121 caggtggtca taggtaacca tgtggccgat gtcttcctgg cggttaacag cgtggcaggc
      181 cttggcgaaa aagcgcaaca ggcaccggaa aatgatgaga aaggtaatct gctaaaccgc
      241 tttgtttatg ttatttcagg tatttttacg cctctgatcg gtctgatggc ggcaaccggg
      301 atcctgaaag gtatgctggc tctggcgctc acttttcagt ggacgaccga acaaagtggt
      361 acttatttaa ttttattcag cgccagtgat gccttgtttg ggttcttccc gataatcctg
      421 ggatacaccg cgggcaaacg cttcggcggt aatccattta ctgccatggt gattggtgga
      481 gcgttagtgc atccattaat tctgactgcc ttcgagaacg ggcaaaaagc ggatgcgccg
      541 gggctggatt tcctgggtat tccggtcaca ttgttgaatt actcgtcatc ggttattccc
      601 attatttttt ctgcctggtt gtgcagcatt ctggaacgcc gacttaatgc gtggttaccg
      661 tcggcaatca aaaatttctt cacaccattg ctatgtctga tggttatcac acccgtcacc
      721 tttctgctgg tggggccgct atcaacctgg ataagcgaaa tgattgccgc cggttatctc
      781 tggctttatc aggcggttcc tgcatttgcg ggcgcggtaa tgggcggctt ctggcaaatc
      841 ttcgtcatgt tcggactgca ctggggcctg gtgccgctgt gtatcaataa cttcaccgtg
      901 ctgggctacg acaccatgat cccgctgtta atgcccgcca ttatggcgca ggtcggggcg
      961 gcgctcggcg tcttcctctg cgaacgcgat gcgcagaaaa aagtggtggc gggatcagcg
     1021 gcgttgacgg gtctgtttgg tatcaccgaa ccagcggtat atggcgtcaa cctgccgcgt
     1081 aagtacccct ttgttatcgc ctgtatcagt ggagcattgg gggccaccat cattggctac
     1141 gcgcaaacga aagtttactc ctttggtttg ccaagtattt tcaccttcat gcagaccatc
     1201 ccgtcaacgg ggattgattt caccgtctgg gccagcgtta ttggcggtgt cattgccata
     1261 ggttgcgcat ttgttggtac gttgatgctt catttcatca ccagtaaacg tcagccagcg
     1321 cagggtgccc cgcaagagaa aacaccagag gttattacac cacctgagca gggcggtatc
     1381 tgttcaccga tgacgggaga gattgtgccg ctcattcacg tcgctgatac cacgtttgcc
     1441 agtggcctgc tgggtaaagg tatcgccatt ctgccctcgg ttggtgaagt gcgttctccg
     1501 gttgcgggtc gaattgcttc gttgttcgcc acattacacg ccattggcat tgagtcagat
     1561 gatggtgtgg agatcctgat tcatgtcggt atcgacaccg taaaactgga cggcaaattc
     1621 ttttccgctc acgtcaacgt gggtgacaag gtcaatacag gcgatcggct gatttctttt
     1681 gatatccctg ctattcgcga ggccggattt gatctgacga cgccggtatt aatcagtaat
     1741 agcgatgatt ttacagacgt attaccccac ggcacggcgc agataagcgc aggtgaaccg
     1801 ctgttatcca tcattcgcta acgataaaag gagttaatta cgaaagcatt tccagaaaca
     1861 tttctttggg gtggcgcaac agctgccaat caggtggaag gtgcctggct ggaagatggc
     1921 aaagggatca cgacctcaga tttacagcct catggcgtaa tgggaaaaat ggaaccgcgc
     1981 atcctgggga aagagaatat caaagatgtc gccatcgatt tttatcaccg ttacccggaa
     2041 gatatcgcgt tatttgccga gatgggcttc acctgtctgc gtatttccat tgcctgggcg
     2101 cgaattttcc ctcagggcga cgaagccgaa ccgaatgaag cggggttagc gttttacgat
     2161 cggctgtttg atgaaatggc gcaggcgggg atcaagccgc tggtaacgtt atcccattac
     2221 gaaatgctat atgggctggt gaaaaactac ggcggttggg ctaatcgggc ggtcatcgat
     2281 cacttcgagc attacgcccg cacggtcttt acccgctacc aacataaagt agcgttatgg
     2341 ctgacgttta atgaaatcaa tatgtcgtta cacgcgccat tcacgggcgt ggggctggca
     2401 gaagagagtg gcgaggcgga agtttatcag gcgatccatc atcaactggt tgccagtgcg
     2461 cgggcagtta aagcctatca tagcctgatc cccgaagcga aaatcggcaa tatgcttctc
     2521 ggtgggctgg tttaccccct cacctgccag ccacaggata tgttgcaggc catggaagag
     2581 aaccggcgct ggatgttctt tggtgatgtt caggcgcgtg gccagtatcc cggctatatg
     2641 cagcgtttct tccgcgacca caatatcacc attgagatga ctgaaagtga cgcagaagat
     2701 ttaaaacata ctgtagattt catctctttt agttattaca tgactggttg tgtttcccac
     2761 gacgaaagca ttaataaaaa tgcgcagggc aacatactga atatgatccc caatccgcat
     2821 ctgaaaagtt cagagagggg gtggcaaatt gatccggttg gattacgggt tctgttaaat
     2881 acgctttggg atcgttatca aaaaccgtta tttattgtcg agaatggatt aggcgtaaaa
     2941 gacagcgttg aagcggatgg ttcgatacag gatgattatc gaattgccta tttaaacgat
     3001 cacctggtac aggtaaatga agcgattgcc gatggtgtgg atattatggg gtacaccagt
     3061 tgggggccaa ttgatttagt cagtgcatct cattcacaaa tgtctaagcg ctacggcttt
     3121 atttatgtgg atcgtgatga taatggcgaa ggaagcctca caagaacgcg taagaaaagc
     3181 ttcggatggt atgcagaagt gatcaaaacg cgggggctgt cattaaaaaa ataaccaata
     3241 aagcacctta attatcgtcg cattcagaac agtctggatg cgatacgtta attctttctt
     3301 tgcaccataa agggatatta tgtttagacg aaatcttatt acctctgcca tcttattaat
     3361 ggcaccgtta gccttttctg cacaatcatt ggctgaatca ttaacggtgg aacaacgcct
     3421 tgagttatta gaaaaagcgt taagagaaac gcaaagcgaa ctcaaaaagt ataaagatga
     3481 agagaagaaa aaatatacgc cagcgacggt gaatcgtagc gtaagtacga atgatcaagg
     3541 gtatgccgcc aatccgttcc cgaccagtag tgccgcaaaa cctgatgctg tactggtcaa
     3601 aaatgaagag aaaaatgcca gtgagacagg ctcgatttat tcttccatga ctctgaaaga
     3661 tttcagtaag tttgtgaaag atgaaattgg ctttagttac aacggctatt accgttctgg
     3721 ttgggggacc gcctctcatg gttcacctaa atcatgggcg attggttctc tgggccgctt
     3781 tggtaacgaa tactccggct ggtttgattt gcagttaaaa caacgtgtct acaacgaaaa
     3841 cggcaaacgg gttgatgccg ttgtgatgat ggatggtaac gttggtcagc agtactctac
     3901 cggctggttt ggcgataacg ccggtggcga gaactttatg cagttctccg atatgtacgt
     3961 taccaccaaa ggtttcctgc cctttgcgcc agaggctgat ttctgggtgg gtaaacacgg
     4021 tgcgccgaaa attgaaatcc agatgctgga ctggaaaacg cagcgtactg atgccgcagc
     4081 gggtgtaggt ctggaaaact ggaaagtcgg tccgggtaaa attgatatcg cgctggttcg
     4141 cgaagatgtc gatgattacg atcgcagcct gcaaaacaaa cagcagatta atacccatac
     4201 tattgattta cgctataaag atatcccgtt atgggataaa gcgaccttaa tggtgagtgg
     4261 tcgttatgtc acggcaaacg aaagcgcatc ggaaaaagat aatcaggata ataacgggta
     4321 ttatgactgg aaagatacct ggatgttcgg cacatcttta acgcagaaat ttgataaagg
     4381 tggcttcaac gaattctcct tcctggttgc gaataactct atcgccagga actttggccg
     4441 ttatgctggc gcaagtccat ttaccacctt taatggtcgt tattatggtg atcacaccgg
     4501 cggaacagca gttcgtctga cctcgcaggg cgaagcctat atcggcgatc attttattgt
     4561 ggctaacgcg attgtttact ccttcggtaa cgatatttat agctacgaaa caggcgcaca
     4621 ctctgatttc gaatctattc gtgcggttgt tcgcccggcc tatatttggg accaatataa
     4681 ccagacaggt gttgaactgg gctatttcac ccagcaaaac aaagatgcga atagtaataa
     4741 atttaatgag tctggttata aaaccacgct cttccatacc tttaaagtca ataccagtat
     4801 gttgacctcg cgtctggaaa ttcgtttcta cgccacgtat atcaaagccc tggaaaacga
     4861 actggatggc ttcaccttcg aagacaataa agacgaccag tttgctgtcg gtgcccaggc
     4921 tgaaatctgg tggtaatgga acttaatgaa aggaatatta cgatgaatat aaaaattgct
     4981 gctttaacgc tggctattgc cagcggtatt tctgctcagt gggccatagc agcggatatg
     5041 ccagccagcc cggcacccac tatcccggtg aaacagtatg tgactcaggt caatgccgat
     5101 aacagcgtga cctttcgcta ctttgcccct ggggcaaaaa atgtctctgt agtggtgggt
     5161 gttccggttc cggacaatat tcacccgatg accaaagacg aagcaggagt ctggtcgtgg
     5221 cgcacaccta tcctgaaagg caacctgtac gagtattttt tcaatgttga tggtgtacgc
     5281 agcattgata caggcaccgc aatgactaag cctcagcgcc aggttaactc cagtatgatt
     5341 ctggtgctag gcagttatct ggatacgcgt tctgttgcgc atggtgattt gatcgccata
     5401 acttaccact ccaacgcatt gcaatctgaa cgtcagatgt atgtctggac cccgccagga
     5461 tacaccggca tgggcgagcc tttgccagtg ctctatttct atcacggttt tggtgatact
     5521 ggacgttccg ctatcgatca ggggcgtatc ccgcaaatca tggataacct gctggctgaa
     5581 gggaaaatta aaccgatgct ggtggtgatc ccggataccg aaaccgatgc gaagggcatt
     5641 attcccgaag atttcgtgcc tcaggaaaga cgtaaagtct tttatccgct gaatgctaaa
     5701 gcggcagatc gcgaactgat gaacgatatt atcccgctga ttagcaagcg ttttagtgtc
     5761 cgtaaagatg ccgatggtcg cgcgctggca gggctttcac aaggcgggta ccaggcgctg
     5821 gtttccggaa tgaatcatct ggaaagcttt ggctggctgg ccacattcag tggtgttacc
     5881 acgacaaccg taccggatga aggtgtcgcg gcccggctga acgatccggc agctattaac
     5941 cagcaactac gtaattttac tgtggtcgtt ggtgataaag atgtcgtaac cggcaaggat
     6001 atcgccgggc tgaaaactga gcttgagcag aaaaaaatta agtttgatta ccaggaatat
     6061 cccggcctga accatgaaat ggatgtctgg cggcctgcct atgcagcctt cgtacagaaa
     6121 tttaaaataa ggcattaaga tgaaattaat cattaccgaa gattaccagg aaatgagccg
     6181 tgtcgcggca caccatctgc ttggttatat gtcgaagacg cgtcgtgtta acctggcaat
     6241 taccgccggt agcacgccca aaggcatgta tgaatacctc accaccctgg ttaaaggtaa
     6301 gccttggtac gataactgct atttctataa tttcgatgaa attccctttc gcggcaaaga
     6361 gggagaaggc gtaacgatta ccaatctgcg taatctgttt ttcacccctg cggggatcaa
     6421 agaagagaat atccagaagc tcactattga taactaccgc gagcatgatc agaaactggc
     6481 gcgtgaaggc ggcctggatt tggtggtgct gggattaggt gcagatggtc atttttgtgg
     6541 caatttgccg aatacgaccc atttccatga gcaaaccgtg gagttcccga tccagggaga
     6601 gatggttgat attgtggcgc atggtgagtt gggcggcgac ttttcgttag tgcctgacag
     6661 ctacgtgacg atggggccga aaagcatcat ggcggcgaaa aacttgctga taattgtcag
     6721 tggcgcaggt aaagcgcagg cactgaaaaa tgtgctgcaa ggtccggtta ccgaagatgt
     6781 tcctgcttcc gtgttgcagc tgcatccttc gctgatggta atcgctgata aagccgctgc
     6841 cgcagaatta gcgttcggtt aagctgaaga aataaggccg gatgtatgaa ttacgctccg
     6901 gcctgttctc attatttaaa taactacgaa aaatcacccc acagtctcag cttgccgcag
     6961 tggagacagc ggaagagata accctgacaa tcaccaccct tttgcaggca tttagctaga
     7021 tcagaatttc taataccgaa attctcacaa tcttcttcaa ggttggcaaa ttcatcgagg
     7081 cgatctttta tatcattcca gcccacatag ccgataaaag cacagaaatc gccacaatgc
     7141 gcaagccaga attcctgttg ccatccatga taacctggcg tgcgttcaac caactctttc
     7201 agcatttcat caggatatgt gttcttcata ccggcaaatt cgtcctcttc atcatactca
     7261 aattcaacac cttctatgct ggcatcatcc tgaaaactac ctgcaaactt ttcagcagca
     7321 gaaccgtccg caatacacaa cggacagaga tgttcaactt catcaacgca ataaaaggga
     7381 cccgagtaat aaactgacgt ctgttgttca cagcaatcgc actctacggt tttatcctgt
     7441 tcaaatgcgc ctgtttccag tggcttggga tgatatttga attggggtaa cggcctgata
     7501 ttttgagtca tagtaacatc ctgtctgctt ttgagtagcc caaagataaa gtatcccgct
     7561 taacttaaag agtctgtttc aggacatgaa atatccaatc cgggcagacg ggtaatgatt
     7621 ctgctcatca ccacacctgc cagcaatgca ggaatgatgc ggacaacgag actgtcacca
     7681 gaaagaatgt tttcaaacaa ctccgaggaa gccatgccct taatatgtac aataactgca
     7741 caatagatct cggtgataac gacgccacct atgccaccag ccagacaacg ataatttttc
     7801 tgtgacccga tcttttccgg cagacaggcg accatcacgc cggttaacaa tgcaggaata
     7861 gctcccgtag taatcgacaa taagagaaac aatggaagta cctgtattat taccaaaaga
     7921 accagcggtt cttttgcgaa gaatatgtag aggacaagaa acagaacacc aattaacggt
     7981 cctaaaaccg caaaataaag tccgtgatgt attacccgac gtgaaacaga catatcaaac
     8041 tccctttact tacctttgag cgccaattta agcggggtaa gtgaagggag tgtgaagaac
     8101 tatgccgtaa tatcccaacc acgcgctttc cacagttccg gtaactgcga aagatgggta
     8161 aaggtggtga ctttcgggtg aacgatcggc ttattgtgcg ggtcggcgca gaagtagaac
     8221 acttccatac ctgcgtcgat accagattgc gcaccggcaa ctgagtcatc aaccagaatg
     8281 cagttttcta catttacatt catcgctttt gccgcatgga acattaacgc cgggtctggc
     8341 ttccagcgct gaatatcgta gccgctgaac agtttatccg ggaagtagtg caacatattc
     8401 agcttgccca tagaatgctg cattttgtta tttgggccgt tagataccac acacattggc
     8461 gcagtgatcg ctgacaggag cgctccagcc ccttcgatgg cttccagttc tgaatcgaac
     8521 agccgagcga cttctgcacg gtaaacgtgt tcagcttctg ttttcgctaa cgtaacacca
     8581 tgttcaaggg aaacaatatc gataatttcg tacagtttta cacctttgaa acgtttgaat
     8641 acctcttcag gatcgagcgt aataccaaat tcctgaaaca tcgttacata tgcgcgggag
     8701 caaatgactt cactgtcgac cagcgtaccg tcgcagtcga aaaataccgc ttctatccgg
     8761 gacatgcctt tcctttttaa tcagtttaac gttttcgtag agccgatttg cgcaacgcaa
     8821 tcgttgccgt ataagtaaat ccaataaaaa gtctctcaca aacgcgccta ttgtcgcatt
     8881 ttggtatacg atagcgacgg attttccctc cttgttcgga aatagataat gagtcatcaa
     8941 cataccaccc agacttctgg tcagaggatg ctggaacgcg tttttaaact gcgcgaacat
     9001 ggtactacgg cacgaactga agtgattgcc gggttcacca ccttcctgac gatggtctac
     9061 atcgtatttg ttaacccaca aattctgggc gttgctggca tggacaccag tgccgtcttc
     9121 gtaacaacct gtctgattgc tgctttcggt agcattatga tgggattgtt tgcaaacctt
     9181 ccggtggcgc tggctcctgc gatggggctt aacgcctttt tcgcttttgt ggttgtccag
     9241 gcaatggggc tgccgtggca gatcggtatg ggcgcaatct tctggggcgc tgtcggactg
     9301 cttcttttga ccattttccg cgttcgttac tggatgattg ctaacattcc ggtaagtctg
     9361 cgtgtgggta ttaccagcgg tatcggcctg tttatcggca tgatggggct gaaaaacgca
     9421 ggtgtgattg tcgctaactc ggaaacgctg gtgagcatcg gtaatctgac ttctcacagc
     9481 gtacttctgg gtatcctcgg ctttttcatc attgctattc tggcctcgcg caacattcac
     9541 gcagcggtgc tggtttctat cgtggtgacg acgctgctgg gctggatgct gggtgatgtg
     9601 cactacaatg gcatcgtttc tgcgccgccg agcgtaatga cagttgtggg tcatgtagat
     9661 ttagccgggt cgtttaacct cgggctggca ggggtgattt tctctttcat gttggtcaac
     9721 ttgtttgact cctccggtac gctgattggc gtgaccgata aagcaggtct ggcggatgag
     9781 aaggggaaat tcccgcgcat gaagcaggcg ctgtatgtcg acagtatctc ttccgtgacc
     9841 ggttcgttta tcggtacttc ttccgttacg gcttatattg agtcctcttc cggcgtatcg
     9901 gttggcggtc gtaccggtct gacggcagtg gttgttggtc tgctgttcct gctggttatc
     9961 tttctgtcgc cgctggcggg aatggtgcca ggctacgctg cagctggcgc gttgatctac
    10021 gttggcgtgt tgatgacctc aagtcttgct cgcgtgaact ggcaggatct tactgaatct
    10081 gttccggcgt ttattaccgc cgtgatgatg ccgttcagct tttcgattac cgaaggtatt
    10141 gcgctgggct ttatctccta ctgcgtgatg aagattggta ccgggcgtct gcgtgacctt
    10201 agcccgtgcg taatcatcgt tgcgctgctg tttatcctga agattgtatt tatcgacgct
    10261 cattaaaatc gatagcgcat taaaaacccg ccatgcatga ttactggcgg gttttttatt
    10321 tagatcttaa ctcgctgaat aaactcacca aatgcggtca attgcccggt caggtggtcc
    10381 agcgtaccct gatcaatcac ttctccggtt tgcggatcaa ctttgttctg aatcacgccg
    10441 cccataaatt ccggcttgtt catcaccatt gcatcgagga aaaccagaat ctggcgcagg
    10501 tgatactgac agcgcgcgcc gccaatcacg cccattgagc tggtctgaat caacaccggt
    10561 ttaccggcca gcggttgatc cggcaggcgg gaaagccagt cgatggcatt tttcagccca
    10621 cccggtaccg agtagttata ttccggcgtg acgatcacca caccgtcagc ctgacggatc
    10681 tgttccgcca gagcttcaac cgttgctgga aaaccttctt cctgctgtac gtcagcgtca
    10741 tacaggggaa tgtcggcaat ggaaggtaac gcattgactt ccatgctcgc cggagcaatt
    10801 ttcggcaggg tacgtgcaac catgccatta aatgagcctt tgcgcaggct ccccagtaac
    10861 gtaaccacct gcaatttttc agacataatg actcctgtgg ttgtctatca atttggcgaa
    10921 aaagacttca cggtagagag ctgggcaaaa cgcatcatcc ggctggtagg attgttggcg
    10981 cggctgtgaa tatccggcag gcttttccag ctgtgtttgc catctaactc ccagacactg
    11041 agcttttcaa tcgcgggcgt gaccgccacg gaccacacca gcacgtcctc cgcgtcgcat
    11101 aacgcttcta catgatttac atgagcacat ttcaggcgtc cggcaatggg cagcagctgc
    11161 agatcgcgcg gatcgtcatt caaaacatca ccggttagtt ttagcacgct tcggcgcagc
    11221 gtaatgagct gcgcccgcgc ttcgttagga tcgttttgtt tactgatcca tagcgattca
    11281 ttgctggaaa aggtgtggtt atcgggcgcg tgtgggctat gaaacccgcg cgtcgcacgc
    11341 tgtagttcca tatcgaggcc acattcacct tcggttgtta acgccacgcc aaccatattc
    11401 ccggcatagg aaatagaaaa cgaaggcaaa tttttatcac tgaaaaccgg tttacctttc
    11461 ggcagggtga cgatttccgg caattcgcca atgccataca gcatgaacat cagttctgcg
    11521 agtaaacctc tggacgccag aaaacggctt tgacgatgag ggggaatgtt tcgggcttct
    11581 tgatgacact gggaggggag acgaacagaa atcagatgtc cttccgttaa aatccctcgg
    11641 gcaaagtgag tcgccatttt tcgctccatg cttaatggtc cattatgaat acaagagtaa
    11701 tcatttactg gaatgatgga cagactaaag cggctaaaag ttcgattcag acaagcttat
    11761 tacaactttt ttacatttta ttgtgaaatt gggtcgcgtt tcatacgatt cggttatgcc
    11821 atcgacgcgt aaaggttttt aatggtttcc cgtaaccaga cgatcttcgg attatggctg
    11881 ttccgtttat gccacagcag ggtaaaagga acttccagct ttttttgctg gctttcgtca
    11941 aacgggagag gaagagcaac caacggcagt tgatggagtt gattgtagta ctgacagtag
    12001 cgcggcgcgg tcgccagtag cagattgtcg ggttgcgctg ccataaacag tgactgctcg
    12061 aattccggca ggctcatagc aatcgtgcgt tcgcgcccca gctcctgtaa cacattgtcc
    12121 agcgccctga ggtagcctga gtttaacgga cactccttcc tgaaa
//
LOCUS       DADMEQ010000115        12154 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.116, whole genome shotgun sequence.
ACCESSION   DADMEQ010000115 DADMEQ010000000
VERSION     DADMEQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.116"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            128..997
                     /locus_tag="JNZ05_003224"
     CDS             128..997
                     /locus_tag="JNZ05_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566350.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1200..1553)
                     /locus_tag="JNZ05_003225"
     CDS             complement(1200..1553)
                     /locus_tag="JNZ05_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAY8566351.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1691..3337)
                     /gene="groL"
                     /locus_tag="JNZ05_003226"
     CDS             complement(1691..3337)
                     /gene="groL"
                     /locus_tag="JNZ05_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAY8566352.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3381..3674)
                     /locus_tag="JNZ05_003227"
     CDS             complement(3381..3674)
                     /locus_tag="JNZ05_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAY8566353.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3950..5206
                     /gene="yjeH"
                     /locus_tag="JNZ05_003228"
     CDS             3950..5206
                     /gene="yjeH"
                     /locus_tag="JNZ05_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HAY8566354.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLEALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5222..5698)
                     /gene="fxsA"
                     /locus_tag="JNZ05_003229"
     CDS             complement(5222..5698)
                     /gene="fxsA"
                     /locus_tag="JNZ05_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HAY8566355.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6035..7471
                     /gene="aspA"
                     /locus_tag="JNZ05_003230"
     CDS             6035..7471
                     /gene="aspA"
                     /locus_tag="JNZ05_003230"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HAY8566356.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7589..8890
                     /locus_tag="JNZ05_003231"
     CDS             7589..8890
                     /locus_tag="JNZ05_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY8566357.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9006..9344
                     /gene="cutA"
                     /locus_tag="JNZ05_003232"
     CDS             9006..9344
                     /gene="cutA"
                     /locus_tag="JNZ05_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HAY8566358.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9320..11017
                     /locus_tag="JNZ05_003233"
     CDS             9320..11017
                     /locus_tag="JNZ05_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HAY8566359.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTIDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11054..11629
                     /locus_tag="JNZ05_003234"
     CDS             11054..11629
                     /locus_tag="JNZ05_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8566360.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11736..11811
                     /locus_tag="JNZ05_003235"
     tRNA            11736..11811
                     /locus_tag="JNZ05_003235"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11769..11771,aa:Phe,seq:gaa)
BASE COUNT         2927 a         3133 c         2987 g         3107 t
ORIGIN      
        1 ttttctgttt atatagagtg agcacggcta tttatgttat tttaacaact attactacca
       61 agcccattta tctggcttat aaattaaaat taattattta attgagaaat ggttagggag
      121 aacctacatg gccataagca tcaagggtgt taataccggt gttattcgca agagtaataa
      181 tttcattgca ctggcgctga aaattaaaga acctcgcaat aaagaatcgt tgttctttat
      241 gtctgtcatg gaactgcgcg acctgctgat tgcgctggaa agtcgtctgc atcaaaagca
      301 taaactggat gctgctgcac gccttcagta tgagcaagct cgcgataaag tcattaagaa
      361 aatggcagaa aacatcccag aaattctggt tgatgagctt aaaaatgccg atattaatcg
      421 tcgggttaac acgctggaac ttaccgataa tcaaggcgaa aatcttacct tcgtattaac
      481 gctacatgac ggtagcaaat gtgagttagt cgtcaatgag ttgcagattg aaatgctggc
      541 gcgagcaatc attcatgcaa ttaataatgc ggaaatgcgt gaactggcat tacgtattac
      601 ctcactgtta gatttcctgc cgttatacga cgttgactgc caggacaatg gcaatctgga
      661 atatgacacc tattcccaac cggaatggaa acataacttg ttcgaccatt atctggcggt
      721 gctttaccgc tttaaagatg aaagtggcaa agaacaattt agcggcgcag tggtgaaaac
      781 gcgtgaagca acgccgggta aagaaataga ggcgatcact cgccgaatgc ttgatttcag
      841 cccacgattg aaaaaactcg cgggtgtgcc atgtcaggtc tatgttcgca ccgtagcggc
      901 gaataacgcc caaccgctaa cccaggatca atgcttacgc gcgctgcatc acttacgtgt
      961 acaatccacc agcaaaactg caccacaggc gaaataatct catctttgct aatgcgaggc
     1021 tggagccatc cagcctgaaa tgactgataa cgtgatcgtt ggtcatgccg gatgcggcgt
     1081 aaacgcctta tccggcctac aaattctcaa gcattcaatc agttgcaatg cctgctgtag
     1141 gcctgataag cgaagcgcat caggcaataa tacgtttgtc acccagcctc tggatcacat
     1201 cagttcgggc acttataaac ctgaccgata atctggctat ccgtcgggac aaaactggac
     1261 aacattccct gcgacgggct gctgatgcca taaatcacgt taccgcccat tgcagccgcc
     1321 tggttgcgca gatcgtttgc tgcgccgcgc atagaaccgc cctcttctcc gtgttgcccg
     1381 gaaagccagt tgctttgctt acctgtcgca gtaccaatca gctggcactc tgcgcctggc
     1441 tgctcgtcca caatgcgtac actctgaccg gcagcactca attcgttgct ggagctacaa
     1501 cccgccatca gtagcgcggc accgacaatc cctgctaagt attttacgtg catgttattc
     1561 cccataatca atgagctgga cgccactcgc gtcgtccgtg tctgaatctt atactagaaa
     1621 gatgaccaaa agaaaaaccc ccagacattt ctgcccgggg gtttgtttat ttctgcgaag
     1681 tgcagggcaa ttacatcatg ccgcccatgc cacccatgcc gcccataccg ccagcagcgc
     1741 ctaagtcagc tgcatcgttt ttcggcaggt cggtaaccat gcattcggtg gtgatcatca
     1801 ggccagccac agaagctgcg tactgcagag cagaacgggt tactttggtt gggtccagga
     1861 tacccatgtc gatcatgttg ccgtattctt cggttgctgc gttgtaaccg tagttgccgt
     1921 cgccgccttt aacggtgtta gcaacaacag acggttcttc gccgcagttc aggacgatct
     1981 gacgcagcgg agcttccatt gcacgcagtg caactttgat acccacgttc tggtcttcgt
     2041 tctgaccacg caggtcagcc agtttagacg ctacgcggat cagcgcaaca ccaccaccag
     2101 caaccacgcc ttcttctacc gcagcacggg tcgcgtgcag ggcatcttca acgcgtgctt
     2161 ttttctcttt catttcaact tcggtagcag cacctacttt gataactgca acgccgcctg
     2221 ccagtttcgc tacgcgctcc tgcagttttt cacggtcgta gtcagaagtt gcttcttcaa
     2281 tctgctgacg gatctgagca acacggccct ggattgcagc ttcttcaccc acgccatcga
     2341 tgatagtggt ggtgtctttg ttgatcacaa cacgtttagc ctgacccagg tcttccaggg
     2401 ttgctttttc cagctccata ccgatctctt cagagattac ggtaccgcca gtcagggttg
     2461 cgatatcctg cagcatagct ttacgacgat cgccgaagcc cggagcttta accgcagcaa
     2521 ctttcacgat gccacgcatg gtgttaacaa ccagagttgc tagcgcttcg ccttcaacat
     2581 cttcagcgat gatcagcagc ggtttgcctg ctttggcaac agcttccaga accggcagca
     2641 tttcgcggat gttggagatt ttcttgtcag ccagcaggat gaacgggctt tccagttcta
     2701 ctgcgccagt ttccggcttg ttgatgaagt aaggagacag gtagccacgg tcgaactgca
     2761 taccttcaac cacgtccagt tcgtcctgca gaccggtacc gtcttcaacg gtgataacgc
     2821 cttctttacc gactttgtcc atcgcttcag cgatcagttt acctacggtt tcgtcggagt
     2881 tagcggagat ggtaccaacc tgagcaatcg ctttagaatc agagcacggt acggacagcg
     2941 ctttcagttc ttcaactgca gcggtaaccg ctttgtcgat accacgtttc aggtccatcg
     3001 ggttcatgcc cgcagcaaca gctttcagac cttcagtgat gatagcctga gccagtacgg
     3061 ttgcagtggt ggtaccgtcg cctgcagcgt cgttcgcttt ggaggcaact tctttcacca
     3121 tctgcgcacc catattttcg aacttgtctt ccagttcgat ttcacgagca acggaaacac
     3181 catctttggt gatggtcggt gcaccgaaag atttatccag aactacgtta cggccttttg
     3241 gaccgagggt aactttcact gcatctgcca gtacgtttac gccgcgcagc attttcacac
     3301 gagcgtcgtt accgaatttt acgtctttag ctgccattat ctttattcct taaattcgta
     3361 tgttcagtgt cgtgcgcgga ttacgcttca acaattgcca gaatgtcgct ttcggacatg
     3421 atcaacactt cttcattgtc gatcttctcg gatttcacac cgtagccatc gttgaaaata
     3481 acgatgtcgc caactttcac gtccagcggc ttcacttcgc cattttcaag gatacggcca
     3541 ttgccgacag ccagcacttc gccgcgggtg gatttagccg ctgcagagcc ggtcagaacg
     3601 atgccgccag cagatttagt ttcaacttct ttacgcttga cgatcacgcg atcatgcaat
     3661 ggacgaatat tcattgataa ctctcctttg agaaagtccg tatctgttat gggtgacgcc
     3721 ggagcttacg tggtttcccg gctggtgacc agagaaatgg ggatgaggct tcgccccttc
     3781 aagggggaaa aagaaaaaaa attctgatca gcacaaaatc gggtgaaaac cctgattcac
     3841 ctcacatttc atcgcaattt cttcatcgcc gtcataagcg aatctgattg tgctaccatc
     3901 gaaaatctac gcagttgccc aaaatttggg cgcaatcgga tatcaaccca tgagtggact
     3961 caaacaagaa ctggggctgg cccagggcat tggcctgcta tcgacgtcat tattaggcac
     4021 tggcgtgttt gccgttcctg cgttagctgc actggtagca ggcaataaca gcctgtgggc
     4081 gtggcccgtt ttgattatct tagtgttccc gattgcgatt gtgtttgcga ttctgggtcg
     4141 ccactatccc agcgcaggcg gcgtcgcgca cttcgtcggt atggcgtttg gttcgcggct
     4201 tgagcgagtc accggctggc tgtttttatc ggtcattccc gtgggtttgc ctgccgcact
     4261 acaaattgcc gccggattcg gccaggcaat gtttggctgg catagctggc aactgttgtt
     4321 ggcagaactc ggtacgctgg cgttggtgtg gtatatcggt actctcggtg ccagttccag
     4381 tgctaatcta caaaccgtta ttgccggact tatcgtcgca ctgattgtcg ctatctggtg
     4441 ggcgggcgat atcaaacctg cgagtatccc cttccccgcg ccaggaaata tcgaacttac
     4501 cgggttattt gctgcgttat cagtgatgtt ctggtgtttt gtcggtctgg aagcatttgc
     4561 ccatcttgcc tcggaattta aaaatccaga gcgtgatttt cctcgtgctt tgatgattgg
     4621 tctgctgctg gcaggattag tctactgggg ctgtacggta gtcgtcttac acttcgacgc
     4681 ctatggtgaa caaatggcgg cggcagcatc gcttccaaaa attgtagtgc agttgttcgg
     4741 tgtaggagcg ttatggattg cctgcgtaat tggctatctg gcctgctttg ccagtcttaa
     4801 catttatata cagagcttcg cccgcctggt ctggtcgcag gcgcaacata atcctgacca
     4861 ctacctggca cgcctctctt ctcgccatat cccgaataat gccctcaatg cggtgcttgg
     4921 ctgctgcgtg gtgagcacgt tggtaattca tgccttagag atcaatctgg aggctcttat
     4981 tatttatgcc aatggcatct ttattatgat ttatctgtta tgcatgctgg caggctgtaa
     5041 attattgcaa ggtcgttatc gactactggc ggtggttggc gggctgttat gcgttctgtt
     5101 actggcaatg gtcggctgga aaagtctcta tgcgctgatc atgctggtgg ggttatggct
     5161 gtttctgcca aaacgaaaaa caccggaaaa tggcataacc acataatccg gcgtttcgac
     5221 attaatcctg gcgatcgtct ttatgatcaa ggcggtcgcg gtcatcatcc tttcgctgat
     5281 actcaccatc aaaagtatta ccgccaccgg tcccggcgct aaaaccgccg cctggcatgc
     5341 gagaaaagcg caaatgtggc atcaacttca ctgtcagatg cttttgcacc ggcggcaata
     5401 aaagcagaag accgaggaag tcggtgaaaa agcccggtaa taaaagcagc aaaccggcaa
     5461 taatcagcga aacactttta atcatctccg ccgcagggtt ttcacccgcc gccatttttt
     5521 gctgcatcag cacgaaattc ttaaagccct ggttacgtac cagtgacata ccgataactg
     5581 acgtgaatat aaccagcacg agggtcagca atacccccaa tacatgggca acctgaataa
     5641 agattgaaat ctcaatatag acataaagga aaatggcaat aaaaggtaac cagcgcaaag
     5701 gtttctcctg taatagcagc cggttaaacc cggctacctg aatgggttgc gaatcgcgtt
     5761 cagcttatat tgtggtcatt agcaaaattt caagatgttt gcgcaactat ttttggtagt
     5821 aatcccaaag cggtgatcta tttcacaaat taataattaa ggggtaaaaa ccgacactta
     5881 aagtgatcca gattacggta gaaatcctca agcagcatat gatctcgggt attcggtcga
     5941 tgcaggggat aatcgtcggt cgaaaaacat tcgaaaccac atatattctg tgtgtttaaa
     6001 gcaaatcatt ggcagcttga aaaagaaggt tcacatgtca aacaacattc gtatcgaaga
     6061 agatctgttg ggtaccaggg aagttccagc tgatgcctac tatggtgttc acactctgag
     6121 agcgattgaa aacttctata tcagcaacaa caaaatcagt gatattcctg aatttgttcg
     6181 cggtatggta atggttaaaa aagccgcagc tatggcaaac aaagagctgc aaaccattcc
     6241 taaaagtgta gcgaatgcca tcattgccgc atgtgatgaa gtcctgaaca acggaaaatg
     6301 catggatcag ttcccggtag acgtctacca gggcggcgca ggtacttccg taaacatgaa
     6361 caccaacgaa gtgctggcca atatcggtct ggaactgatg ggtcaccaga aaggtgaata
     6421 tcagtacctg aacccgaacg accatgttaa caaatgtcag tccactaacg acgcctaccc
     6481 gaccggtttc cgtatcgcag tttactcttc cctgattaag ctggtagatg cgattaacca
     6541 actgcgtgaa ggctttgaac gtaaggctgt cgaattccag gacatcctga aaatgggtcg
     6601 tacccagctg caggacgcag taccgatgac cctcggtcag gaattccgcg ctttcagcat
     6661 cctgctgaaa gaagaagtga aaaacatcca acgtaccgct gaactgctgc tggaagttaa
     6721 ccttggcgca acagcaatcg gtactggtct gaacacgccg aaagagtact ctccgctggc
     6781 agtgaaaaaa ctggctgaag tcactggctt cccatgcgta ccggctgaag acctgatcga
     6841 agcgacctct gactgcggcg cttatgttat ggttcacggc gcgctgaaac gcctggctgt
     6901 gaagatgtcc aaaatctgta acgacctgcg cttgctctct tctggtccac gtgccggcct
     6961 gaacgagatc aacctgccgg aactgcaggc gggctcttcc atcatgccag ctaaagtaaa
     7021 cccggttgtt ccggaagtgg ttaaccaggt atgcttcaaa gtcatcggta acgacaccac
     7081 tgttaccatg gcagcagaag caggtcagct gcagttgaac gttatggaac cggtcattgg
     7141 ccaggccatg ttcgaatccg ttcacattct gaccaacgct tgctacaacc tgctggaaaa
     7201 atgcattaac ggcatcactg ctaacaaaga agtgtgcgaa ggttacgttt acaactctat
     7261 cggtatcgtt acttacctga acccgttcat cggtcaccac aacggtgaca tcgtgggtaa
     7321 aatctgtgcc gaaaccggta agagtgtacg tgaagtcgtt ctggaacgcg gtctgttgac
     7381 tgaagcggaa cttgacgata ttttctccgt acagaatctg atgcacccgg cttacaaagc
     7441 aaaacgctat actgatgaaa gcgaacagta atcgtacagg gtagtacaaa taaaaaaggc
     7501 acgttagatg acgtgccttt tttcttgtga gcagtaactt aaaaataaca atataatatc
     7561 aacttgttaa aaaacaagga aggctaatat gctagttgta gaactcatca tagttttgct
     7621 ggcgatcttc ttgggcgcca gattgggggg aataggtatt ggttttgcag gcggattggg
     7681 ggtgctggtt cttgccgcta ttggcgttaa acccggtaac atcccgttcg atgtcatttc
     7741 cattatcatg gcggttatcg ccgctatttc tgccatgcag gttgctggcg gtctggacta
     7801 tctggttcat cagacagaaa agctgctgcg ccgtaacccg aaatacatca cgatcctcgc
     7861 accgatcgtg acctatttcc tgactatctt tgctggtact ggcaacatct ctctggcgac
     7921 actgccagtt atcgctgaag ttgcgaagga acaaggcgtt aaaccttgcc gtccgctctc
     7981 tactgcagtg gtatccgcgc agattgcgat caccgcatcg ccaatctcag cggcagtggt
     8041 ttacatgtct tccgtgatgg aaggtcatgg catcagctac ctccatctgc tctccgtggt
     8101 catcccgtcc accctgctgg cggttctggt gatgtccttc ctggtcacta tgctgttcaa
     8161 ctccaaactc tctgacgatc cgatttatcg caagcgtctg gaagagggct tggttgaact
     8221 gcgcggtgaa aagcagattg aaatcaaatc cggtgcaaaa acgtccgtct ggctgttcct
     8281 gctgggcgta gttggcgtgg ttatctatgc aatcatcaac agcccaagca tgggtctggt
     8341 tgaaaaaccg ctgatgaaca ccaccaacgc aatcctgatc atcatgctca gcgttgcaac
     8401 tctgaccacc gttatctgta aagtcgatac cgacaacatc ctcaactcca gcaccttcaa
     8461 agcaggtatg agcgcctgta tttgtatcct gggtgttgcg tggctgggcg atactttcgt
     8521 ttccaacaac atcgactgga tcaaagatac cgctggtgaa gtgattcagg gtcatccgtg
     8581 gctgctggcc gtcatcttct tctttgcttc tgctctgctg tactctcagg ctgcaaccgc
     8641 aaaagcactg atgccgatgg ctctggcact gaacgtttca ccgctgaccg ctgttgcttc
     8701 tttcgctgcg gtgtctggtc tgttcattct gccgacctac ccgacgctgg ttgctgcggt
     8761 acagatggat gacacgggta ctacccgtat cggtaaattc gtcttcaacc atccgttctt
     8821 catcccgggt actctgggtg ttgccctggc cgtttgcttc ggcttcgtgc tgggtagctt
     8881 catgctgtaa tgatcaatcg cggggcgttc acgccccgct ttctttcccg ccgactaaca
     8941 tccttccccc gtccgttgta tagtgacctc tctcttgcgg ttccatctgt tcttgcgagg
     9001 tgtttatgct tgatgaaaaa agttcgaata ccgcgtctgt cgtggtgcta tgtaccgcac
     9061 cggatgaagc gacagcccag gatttagccg ccaaagtgct ggcggaaaaa ctggcggcct
     9121 gcgcgacctt gatccccggc gctacctctc tctattactg ggaaggtaag ctggagcaag
     9181 aatacgaagt gcagatgatt ttaaaaacta ccgtatctca ccagcaggca ctgctggaat
     9241 gcctgaagtc tcatcatcca tatcaaaccc cggaacttct ggttttacct gttacacacg
     9301 gagacacaga ttacctctca tggctcaacg catctttacg ctgattctgc tactttgcag
     9361 cacttccgtt tttgccggat tattcgacgc gccgggacgt tcacaatttg tccccgcgga
     9421 tcaagccttt gcttttgatt ttcagcaaaa ccaacatgac cttaatctga cctggcagat
     9481 caaagacggt tactacctct accgtaaaca gatccgcatt acgccggaac acgcgaaaat
     9541 tgccgacgtg cagctgccgc aaggcgtctg gcatgaagat gagttttacg gcaaaagcga
     9601 gatttaccgc gatcggctga cgcttcccgt aactatcaac caggcgagtg cgggagcgac
     9661 gttaactgtc acctaccagg gctgtgctga tgccggtttc tgttatccgc cagaaaccaa
     9721 aaccgttccg ttaagcgaag tggtcgccaa caacgcagcg tcacagcctg tgtctgtttc
     9781 gcagcaagag cagcacaccg cgcaattgcc cttttccgcg ctctgggcgt tgttgatcgg
     9841 tattggtatc gcctttacgc catgcgtgct gccaatgtac ccactgattt ctggcatcgt
     9901 gctgggtggt aaacagcggc tctccactgc cagagcattg ttgctgacct ttatttatgt
     9961 gcaggggatg gcactgactt acacggcgct gggtctggta gttgccgccg cagggttaca
    10021 gttccaggcg gcgctacagc acccatacgt gctcattggc ctcgccatcg tctttacctt
    10081 gctggcgatg tcaatgtttg gcttgtttac tctacaactc ccctcttcgc tgcaaacacg
    10141 tctcacgttg atgagcaatc gccaacaggg cggctcacct ggcggtgtgt ttgttatggg
    10201 ggcgattgcc ggactgatct gttcaccatg caccaccgca ccgcttagcg cgattctgct
    10261 gtatatcgcc caaagcggga acatgtggct gggcggcggc acgctttatc tctatgcgtt
    10321 gggcatgggc ctgccgctga tgctaattac cgtctttggt aaccgcttgc tgccgaaaag
    10381 cggcccgtgg atggaacaag tcaaaaccgc gtttggtttt gtgatcctcg cactgccggt
    10441 cttcctgctg gagcgagtga ttggtgatgt atggggatta cgcttgtggt cggcgctggg
    10501 tgtcgcattc tttggctggg cctttatcac cagcctacag gctaaacgcg gctggatgcg
    10561 cgtggtgcaa ataatcctgc tggcagcggc attggttagc gtgcgcccac ttcaggattg
    10621 ggcatttggt gcgacgcata ccgcgcaaac tcagacgcat ctcaacttta cacaaatcaa
    10681 aacgatagat gagttaaatc aggcgctcgt tgaagccaaa ggcaaaccgg tgatgttaga
    10741 tctttatgcc gactggtgcg tcgcctgtaa agagtttgag aaatacacct tcagcgaccc
    10801 gcaggtgcaa aaagcgttag cagacacggt cttacttcag gccaacgtca cggccaacga
    10861 cgcacaagat gtggcgctgt taaagcatct taatgtcctt ggcctaccga caattctctt
    10921 ttttgacgga caaggccagg agcatccaca agcacgcgtc acgggcttta tggatgctga
    10981 aaccttcagc gcacatttgc gcgatcgcca accgtgaacg acacttgcag tgggaaagac
    11041 ggaggaaaat agcgtgcaac gtgaagatgt actgggagaa gccctgaaat tattagaatt
    11101 acaagggatt gccaacacca cgctggagat ggttgctgaa cgtgtggatt atccactgga
    11161 cgagctacgc cgcttctggc cagacaaaga ggcaatcctc tacgatgcgc tgcgttatct
    11221 tagccaacag atagatgtct ggcgtcgtca gctgatgctg gacgaaacgc aaaccgccga
    11281 acaaaagctg ctggcacgtt atcaggcgtt atcggagtgc gttaaaaaca accgctatcc
    11341 gggctgtcta tttatcgctg cctgtacgtt ttatcccgat cctggccacc ctattcatca
    11401 actggccgat cagcaaaaaa gcgcggccta cgatttcacc cacgaactgt taaccacgct
    11461 ggaagttgac gatccggcga tggtagcaaa gcagatggaa ctggtgctgg aaggctgttt
    11521 aagccgaatg ctggtgaatc gtagccaggc ggatgtcgac actgcacatc ggctggcgga
    11581 agatatcctg cgcttcgccc gctgccgcca gggtggtgca ttgacctgac agaaacacag
    11641 aaaagaagcg atttgccgca atcttaagca gttgaatcgc ttttactgaa attaggttga
    11701 cgagatgtgc agattacggt ttaatgcgcc ccgttgcccg gatagctcag tcggtagagc
    11761 aggggattga aaatccccgt gtccttggtt cgattccgag tccgggcacc aaattcatat
    11821 caacggacct ccacggaggt ccgtttttcg tttcaggacg ccacgattta agcgttctgc
    11881 ctccaaatca attctaccga actcaaccag attctcccca catcaccagc aatttgcgag
    11941 catatcccaa tttgggaaaa tttgttcctg agctatagca ctaactgacg tgaaatgtcg
    12001 tgcggccccg tgatgctgtt gaacgtcaaa tgacgtcatc aggagcgtaa cgtatccata
    12061 aagcacaaca ccggtctgaa cgccgactga tgagattttc tgaatgagaa caaagagaaa
    12121 tgtgtcagtc cgcttgctca tgcaaagact aaca
//
LOCUS       DADMEQ010000116        11785 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.117, whole genome shotgun sequence.
ACCESSION   DADMEQ010000116 DADMEQ010000000
VERSION     DADMEQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.117"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            202..537
                     /locus_tag="JNZ05_003236"
     CDS             202..537
                     /locus_tag="JNZ05_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HAY8566361.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            630..713
                     /locus_tag="JNZ05_003237"
     CDS             630..713
                     /locus_tag="JNZ05_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566362.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            813..1634
                     /locus_tag="JNZ05_003238"
     CDS             813..1634
                     /locus_tag="JNZ05_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAY8566363.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1673..2002)
                     /gene="mdtI"
                     /locus_tag="JNZ05_003239"
     CDS             complement(1673..2002)
                     /gene="mdtI"
                     /locus_tag="JNZ05_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HAY8566364.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1989..2354)
                     /gene="mdtJ"
                     /locus_tag="JNZ05_003240"
     CDS             complement(1989..2354)
                     /gene="mdtJ"
                     /locus_tag="JNZ05_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HAY8566365.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2765..3799
                     /gene="tqsA"
                     /locus_tag="JNZ05_003241"
     CDS             2765..3799
                     /gene="tqsA"
                     /locus_tag="JNZ05_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HAY8566366.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMEMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVHFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3824..5212)
                     /gene="pntB"
                     /locus_tag="JNZ05_003242"
     CDS             complement(3824..5212)
                     /gene="pntB"
                     /locus_tag="JNZ05_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HAY8566367.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGGVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARSINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5223..6755)
                     /gene="pntA"
                     /locus_tag="JNZ05_003243"
     CDS             complement(5223..6755)
                     /gene="pntA"
                     /locus_tag="JNZ05_003243"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HAY8566368.1"
                     /translation="MQIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFNE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7279..8223
                     /locus_tag="JNZ05_003244"
     CDS             7279..8223
                     /locus_tag="JNZ05_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY8566369.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            8409..9791
                     /locus_tag="JNZ05_003245"
     CDS             8409..9791
                     /locus_tag="JNZ05_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8566370.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQDAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9828..10550
                     /gene="folM"
                     /locus_tag="JNZ05_003246"
     CDS             9828..10550
                     /gene="folM"
                     /locus_tag="JNZ05_003246"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HAY8566371.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10547..10882)
                     /locus_tag="JNZ05_003247"
     CDS             complement(10547..10882)
                     /locus_tag="JNZ05_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HAY8566372.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            11011..11754
                     /gene="rstA"
                     /locus_tag="JNZ05_003248"
     CDS             11011..11754
                     /gene="rstA"
                     /locus_tag="JNZ05_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HAY8566373.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
BASE COUNT         2995 a         3011 c         2834 g         2945 t
ORIGIN      
        1 gctttgtaag tagtataata gttaacccga tcaagactac tattattggt agctaaattt
       61 tccttaagtc acaatacgtt attatcaacg ctgtaattta ttcagcgttt gtacatatcg
      121 ttacacgctg aaaccaacca ctcacggaag tctgccattc ccagggatat agttatttca
      181 acggccccgc agtggggtta aatgaaaaaa caaattgagg gtatgacaat gaaaaaagta
      241 ttagctctgg ttgttgccgc tgctatgggt ctgtcttctg ccgcctttgc tgcagagact
      301 gcgaccacac ctgctccgac tgcgacgacc accaaagcag cgccagcgaa aactacacat
      361 cataaaaaac agcataaagc agcacctgcc cagaaagcgc aggcggctaa aaagcatcat
      421 aaaaatacga aagctgaaca gaaagcccct gaacaaaaag cgcaggcagc gaagaaacac
      481 gccgggaaac acggccatca gcaaccggca aaacctgctg cacaacccgc agcgtaagtt
      541 ttcaacagta atgctggcgc gccccctcgc gcctgaaaat tacggtgcta agcgggtaac
      601 gtttagcacc gcctttagcg gagggtagta tggtgggccg ttatcgcttt gagttcattc
      661 ttatcatcct tattttatgc gcactgattg ccgcccgttt ttatctttcc tgactgtagt
      721 tatctgattt tactcccact ttcatcccgt cccgtctata gtatttatga gggtttgctt
      781 ttaataatca taattaccca ccagagtgtg atatgcgtac aaccattgct gtagtgttgg
      841 gtgcaattag tttgacgtct gcttttgtgt ttgcagataa accagacgtt gccaaatcgg
      901 caaacgatga ggtcagcacc ctgttttttg gtcatgatga tcgtgtgcca gtgaatgaca
      961 cgacccaatc accgtgggat gcggttgggc aactggaaac ggccagcggc aatttatgta
     1021 cggcgacgct gattgcaccc aatctggcat taacggcagg acactgttta ttgacgccgc
     1081 ctaagggtaa agcggataag gcggtagcgc tgcgttttgt gtcaaataaa ggtctttggc
     1141 gctatgagat ccacgacata gaaggccgcg ttgatccgac actgggaaag cggttaaaag
     1201 cagatgggga tggttggatt gttcctcccg cagctgcacc gtgggacttc ggattgattg
     1261 tgctacgtaa tcccccttct ggcattacac cgttgccgtt atttgaggga gataaagccg
     1321 cgcttactgc cgcattaaaa tcggctggtc gtaaagtgac tcaggcggga taccctgaag
     1381 atcatctcga tacgttatac agtcatcaaa actgtgaagt gactggctgg gcgcaaacgt
     1441 cggtgatgtc acatcagtgc gataccttgc cgggtgacag cggttcgcct ctgatgttgc
     1501 ataccgatga cggctggcaa ttaattgggg tgcaaagttc ggctcctgcc gcgaaagatc
     1561 gctggcgcgc cgataaccgg gccatttctg ttaccggttt tcgcgacaag ctggatcaac
     1621 tgtcgcaaaa ataatgttca aacgctgccc gacagcgcgg gcagcgtctt catcaggcaa
     1681 gtttcaccat gatcattcca gccaacagca agaccaggcc aatccagcct ttacgattta
     1741 accgttgacc aaacaagatc caacctgcgg ctaacgtggc ggcaatacca aacccgcccc
     1801 acaatgcata agcgacagac aagtcgatcc ctttaacggc ctgagaaagc gcactaaagg
     1861 ccgccagcac cgccgccagg gagagcaagc caaatatttt gcgacgaaag ccgtcagaaa
     1921 atttcaaaaa gacgttagca acgatttcca gcacgattgc caatgccagc caggcggcgt
     1981 gaacccattc aaactgcgcc atggttcacc tccagttcag gtttacgcgc tttacgggta
     2041 cctgatttga tcaacactat cccggcgacc agggtggtta acccggcaat tttcatcagc
     2101 gataaacttt cgtcgaataa caaaacgcta aacaaggtaa taaataaaat accgatacct
     2161 tcccacagcg cataagctac gcctaaggcg atttttttaa cggcgaaaga gagaaatata
     2221 tacgacagag aaatcatcac cagcattaaa ataaagccgc catttccctc actgacgctc
     2281 gcccatttca ttgacagcgt accggtaatt tctgtagcaa tagccagacc taataaaatc
     2341 caataaatat acattgtcct tctcctgcaa gagaactatt ttaattttcg cttaattcag
     2401 cgaaaccaaa gtaaagtggt caagccctgc gcatagggca agcttaggca gaagaaagga
     2461 ctaaagcgcg ttgcgccagt gctgctcacc tacgagcaag atagtggatg aggtacgaag
     2521 atgggtaaat gtagaaaata acgtcctgaa caaattgtcc ataatattac aattatccgc
     2581 agtgttgctt ctcgtcatcg cagatgataa ttgtcctcgg tagttgaaca cgcctgattt
     2641 gtatcatagc ttaagaatta actcaaaata ttttcacttc tttacctgag cggtttgatt
     2701 ttcgttatga tgacggagcg aaaaagacat tattattagc aaaggaagaa aaacggggac
     2761 aagcatggca aagccgatca tcacgctcaa tggcctaaaa atcgtcatta tgttgggaat
     2821 gctggtcatt attctctgcg gtatccgttt tgccgccgag atcatcgtgc cgtttattct
     2881 cgcattattt attgctgtta ttcttaaccc gctggtgcaa catatggtcc gctggcgtgt
     2941 gccgcgtgta ctggcagtgt cgattttgat gacaatcatc gtgatggaga tggtgttgct
     3001 attagcttat ctgggttccg cgctcaacga gttgacgcgg acgttaccgc aatatcgcaa
     3061 ctctattatg acgccgctgc aagctcttga gccgttgttg caacgcgtag ggattgacgt
     3121 ctcagttgac caactggcgc attacattga tccgaacgcg gcgatgacgt tgctcaccaa
     3181 cttattgacg cagttatcta atgccatgtc atcaatattt ttattgctgc tgacggtgct
     3241 gtttatgctg ctcgaagtgc cacaattgcc cggaaaattt cagcaaatga tggcgcgtcc
     3301 ggttgaaggg atggcggcga ttcaacgtgc gattgacagt gtttctcatt atctggtgct
     3361 gaaaacagcc atcagcatca tcaccggctt ggtcgcctgg gcgatgctcg ccgcactcga
     3421 tgttcacttc gcttttgtct ggggattgct ggcctttgcg cttaattaca tccctaatat
     3481 tggttcagtt ctcgcggcaa tcccccctat cgctcaggta ctggtgttta atggcttcta
     3541 cgaagcgttg ctggtgctgg cgggatatct gctgattaat ctggtcttcg gcaatattct
     3601 ggagccgcgc atcatgggac gtgggctggg gctttcgaca ttggtggtat ttttgtcgtt
     3661 gattttttgg ggatggttgt taggaccggt gggtatgctg ctttccgtgc cgttgacaat
     3721 tattgtcaaa attgcgcttg aacaaacagc gggaggtcaa agcatcgccg ttctgttaag
     3781 cgatctcaat aaagagtgac ggcctccgca gaggccgtca gggttacaga gctttcagga
     3841 ttgcatccac gctggctttg gcgtcaccaa acagcatgtg ggtgttttcc ttgaagaaca
     3901 gcgggttttg cacaccagca tagccagtgt tcatcgaacg tttaaagaca atcacgttct
     3961 gcgctttcca cacttccagc acaggcatac cagcaatcgg actcttcgga tcatcctgcg
     4021 ccgccgggtt aaccgtatcg ttagcaccaa tcaccagtac ggtatcggta tcagcaaagt
     4081 catcgttgat ctcgtccatt tccagcacga tgtcatacgg tacttttgct tcagccagca
     4141 atacgttcat atgtccaggc aaacgccccg caaccgggtg gataccgaaa cgcacattaa
     4201 tactacgagc gcggagtttc tcggtaattt cagcgacagg atattgcgcc tgcgcgactg
     4261 ccatgccgta ccccggagta atgatcactg aatgggagtt tttcagcagt tccgctgtct
     4321 cttctgcggt gatttcgcgg tgctcaccca cttcctgatc atcgccagta gaagagccgt
     4381 cggtgccgaa accacccgca ataacgctga taaaggaacg gttcatcgcc ttacacataa
     4441 tgtaagaaag gatagccccc gaagaaccga ccagcgcacc ggtcacaatc agcaggtcgt
     4501 tgctgagcat aaagcccgca gccgcagccg cccagccgga gtacgagttc agcatcgaca
     4561 ccaccaccgg catatctgca ccaccgatgg aggcgactaa atgccagccg aataccagcg
     4621 caatggcggt cattatcagc aatgccagca cttgcaggcc gacgctgtcc gtgcgaacaa
     4681 atacaatcag cagcaagaag gaaacgacca gagccgccag gttcattttg tgacggtttg
     4741 gcagcatcaa cggtttagac gaaatcttgc cacacagttt gccgaacgcc accaccgagc
     4801 ccgtgaacgt tacccccccg atgaagatac cgaggaacac ttccgtcagg tgaatattga
     4861 ccagaatcgg tgccattccc gcgtcatgat gcagataact gttaaagcca accagcactg
     4921 ccgccagacc cacgaagctg tgcaggatcg ccaccagttc tggcatttcg gtcatttcaa
     4981 ctttcttcgc cagccggata ccaattgccc caccaatgac catcgccagc aagatccagc
     5041 caacattacc cgtatccggt ccaaaaatgg ttgcgattaa cgcaatcgcc atcccggcga
     5101 taccgaagtt gttaccctgg cgagacgttt catgtttcga aagaccggcc agactgaaga
     5161 taaacaggat cgcggcaaca atgtatgcag ctgtaactaa tcctccagac atatgttacc
     5221 ccttaatttt tgcggaacat tttcagcatg cgctgagtca cggtgaagcc accgaaaata
     5281 ttaatgctgg ctataagcac cgcgataaaa ctaaggaagc taacccagcc tccctggcca
     5341 atctgcaaca gtgctccgac aacaataatc cctgaaatcg cgttggtaac cgacatcaac
     5401 ggtgtatgca gcgcgtgcga tacattccac accacgtaat aaccgacaac gcaggccagc
     5461 gcgaaaacgg tgaagtgccc aagaaactct ttcggcgcaa cgcttgacag ccaaccgaac
     5521 aggatgattg ccagcgccat caacgcgtat ttacgccacg gtgagcaggc acatttttcc
     5581 tcagttttca cttccggtgc cgctttttgt gccgcctgcg gctgagctga tacctgaatc
     5641 ggcggtgccg gccaggtaat ttcgcccgca cggatcacgg tcacgccgcg aatcaccaca
     5701 tcatcaaaat caacagtgat attgccgtct ttctctttgc acaacagttt cagcagatta
     5761 acgaggttag taccgtaaag ctgtgaggat tgcgtcggca gacggcccgg aagatcggta
     5821 taaccaatca ctttgacacc attttccgta ctgaagattt cacccggcac gatgtattca
     5881 cagttgccac cgttttgggc tgccaggtcg acaatcacac tgcccgcctt catggagtca
     5941 accatttcac gggtaattag cttcggcgct ggtttgcctg gaataagcgc ggtggtgaca
     6001 atgatatcga cctcttttgc ctgggcggca aagagttcca tttccgcttt gatgaacgcg
     6061 tccgacatca ctttggcata gccatcgccg ctgcccgctt cctcgttaaa atccagctcg
     6121 aggaattccg cgcccatact ttgaacttgt tctttcactt ccgggcgggt gtcgaatgca
     6181 cgcacaatcg cgccgagact gtttgctgcg ccaatggcgg ccaaacctgc aacaccggca
     6241 ccaatcacca tcacttttgc cggtggcact ttcccggccg cagtgatttg cccggtaaag
     6301 aagcgcccaa attcatgtgc cgcttcaaca atggcgcgat aaccggcgat attcgccatc
     6361 gagcttagtg cgtccagcga ttgtgcgcgt gagatacgcg gcacagagtc catcgccatc
     6421 acggtcacgt tacgttccgc aagtttttgc attaattccg gattctgcgc aggccagata
     6481 aaactcacca gcgttgtccc gggattcagt aacgcaattt catcatctaa cggcgcattg
     6541 accttcagaa tgatctctga ctgccagacg ctattccctt ctacaatttc agcgcccgct
     6601 tgcacaaacg ctttatcgtc aaaacttgcc agttgacccg cgccgctctc taccgcgacg
     6661 gtaaaaccca gtttcagcag ctgttccact gtttttggcg ttgctgcaac acgggtttca
     6721 ttggttaacc gttctcttgg tatgccaatt tgcatgatat tcccttccat cggttttatt
     6781 gatgatggtt tgcctgtgtc aggagccaca caagccgctc atgtacgagc taaatgttac
     6841 tccgttaaaa taaattagta acaaacgccc tataacgtac tgaaaattat gcctgtgatc
     6901 tagcgccaaa aatcagtatt tcggcgtgaa ctcgcaaaat attaacgatt cagccgtgat
     6961 agtgggataa acaccttaga acgccggata aagactgata attgtcttcg acggtcgggt
     7021 aaaacgagac atcgccccgg cacgaatcac tacttaacat taaattaact tatacaattc
     7081 agttgcttca gtagtaatga tgctgatacg gctgtttttt aagcatagac ggtcatttga
     7141 gcaggattaa aattggttta aggaatgtga tatgaaaaat gacgcagaca gttacaccgt
     7201 ttaaatgcaa taatcagcca cgtttctcgt taataacaat accagtacct ggtttgcgca
     7261 aggcgaagga ttatttttat gaagcttaag aacaccctcc tggcgtcggc actgctttct
     7321 gctacggcat tctccgttaa cgcagcaaca gaactgacac cggagcaagc ggcagcggtt
     7381 aaaccttttg accgtgtagt ggttaccggt cgttttaatg ctattggcga agcggtgaaa
     7441 gccgtttctc gtcgcgcaga taaagaaggt gccgcctctt tttatgttgt cgacacttct
     7501 gattttggta acagcggtaa ctggcgtgtg gtcgctgacc tctataaagc cgatgctgaa
     7561 aaagcagaag aaacaagtaa tcgcgtaatt aacggtgttg tcgaactgcc gaaagatcag
     7621 gctgttctga ttgaaccgtt tgacacggtc accgtccagg gcttctatcg tagccagcca
     7681 gaagtcaatg atgccatcac caaagcggca aaagcgaaag gtgcctactc tttctacatc
     7741 gttcgtcaaa tcgatgccaa ccagggcggc aaccagcgta ttactgcatt catctataaa
     7801 aaagatgcta agaaacgtat cgtccagagc ccggatgtga tcccggcaga ttccgaagca
     7861 ggacgtgcag ctctggctgc cggtggcgaa gccgcgaaga aagttgagat cccgggtgtt
     7921 gcgactaccg catcaccaag ttctgaagtc ggtcgcttct ttgaaaccca gtcatcaaaa
     7981 ggcgggcgtt acaccgtcac gctcccggat ggcactaaag tcgaagaact gaacaaagcg
     8041 acagcagcga tgatggtccc gttcgacagc atcaaattct ctggcaacta cggcaacatg
     8101 accgaagtct cctatcaggt tgcgaaacgt gccgcgaaga aaggtgctaa gtattaccac
     8161 atcacccgcc agtggcagaa acgtggtaat aacctgaccg tcagcgcaga tctgtataaa
     8221 taactattca gttactccta taaggcggct tgatgccgcc tttcgcattt ttagtcacaa
     8281 atttccccgc gaccattgca tgcagacaca tctcttcgta aaatcccgcg cttcagtggc
     8341 aaactccatt tttatgcgtt ttgccctaat atttattctt cttatcacgt tttaatcact
     8401 ggatatcgat ggaaaagaaa ctgggactga gcgcactcac cgcgctggta ttaagctcaa
     8461 tgctgggcgc gggtgttttc agtctgccgc aaaatatggc ggcagttgcc agcccggcag
     8521 cactgctcat cggctggggt attactggcg ctggcatttt attgctggcc tttgccatgc
     8581 tgatcctcac gcgcattcgt cccgaacttg atggcggtat ctttacctat gcccgcgaag
     8641 ggtttggcga gctaatcggc ttttgttccg catggggata ctggctgtgc gcggtcatcg
     8701 ccaacgtctc ctatctggtg atcgtttttt ccgcgttaag cttttttacg gacacgccgg
     8761 aattgcgcct gtttggcgat ggtaatacct ggcaatcgat agtcggtgca tcggctttat
     8821 tgtggattgt tcactttttg atcctgcgcg gggtgcaaac cgctgccagc attaacctgg
     8881 tggcgacact ggcaaaattg ttgccgctgg gtctgtttgt tgtgctggcg atgatgatgt
     8941 tcaaactgga taccttcaag ctcgacttca ccggacttgc acttggcgta cctgtttggg
     9001 aacaggtgaa aaacaccatg ctgatcaccc tgtgggtttt cattggtgtg gaaggtgccg
     9061 tggttgtttc tgcgcgtgcg cgtaataaac gtgatgttgg caaagcgaca ctgctggcgg
     9121 ttctctccgc tctgggcgtt tacttgctgg taacgctgct ttcgctgggt gtggtggcac
     9181 gtcctgaact ggctgaaatt cgtaacccgt caatggccgg tctgatggtg gaaatgatgg
     9241 gaccatgggg cgaaatcatc atcgctgccg gtttgatcgt ttccgtttgc ggcgcgtact
     9301 taagctggac catcatggcg gcagaagttc cgttcctggc tgccactcat aaagcattcc
     9361 cacgcatttt tgcgcgccag gacgcacaag ctgcgccatc cgcctcactg tggttgacca
     9421 acatctgcgt gcaaatttgt ctggtactca tctggctgac cggttccgat tacaacacgt
     9481 tgctgaccat cgcctcagaa atgattctgg tgccctattt cctggtcggc gcgttcttgc
     9541 tgaaaatcgc aacccgtcca ctgcataaag cggtaggtgt cggtgcctgc atttatggct
     9601 tatggttatt gtacgcctct ggtccaatgc acttgctgtt atccgttgtt ctgtatgcac
     9661 cggggctgct ggttttcctg tatgcgcgca aaacgcatac acatgataat gtactgaacc
     9721 gtcaggaaat ggtgctgatt ggcatgctgt taattgcctc agttccggcg acctggatgc
     9781 tggtgggata acgttcatcc catcgttgga tagcaaagga gataacgatg ggtaaaaccc
     9841 agcccttgcc aatattaatt actggcggag gtcgtcgcat cggcctcgcc ctcgcatggc
     9901 atttcattaa tcaaaagcaa ccggtgattg tcagctatcg gacacactat ccagccattg
     9961 atggactgat taatgcaggt gcgcagtgta ttcaggctga tttttcgacc aacgacggtg
    10021 tgatggcgtt tgccgatgaa gtactaaaaa ccacccatgg tctgcgtgct attttgcata
    10081 acgccagtgc gtggattgcg gaaaaaccgg gtgcgccact ggccgacgta ctggcttgca
    10141 tgatgcagat ccacgttaat accccatacc tgctcaacca tgcgctggaa agattactgc
    10201 gtgggcacgg acacgccgcc agcgatatca ttcactttac cgattatgtg gtggagcgcg
    10261 gtagcgacaa acatgttgcg tatgctgcaa gcaaagcggc actggataat atgacccgct
    10321 cgtttgcccg caagctggca ccggaagtga aagtgaattc tattgcgcca tcgctgatcc
    10381 tgtttaatga acatgatgat gccgaatatc gacaacaggc gctgaataaa tcactgatga
    10441 aaaccgcgcc tggcgagaaa gaagtgatcg acctggtcga ttacttactt accagttgct
    10501 ttgtcaccgg acgcagtttc ccacttgatg gcggtcgtca tctgcgttaa tgcagcttta
    10561 tccagcatat aatcaacacc catgcgctta tcccccaaca cgcaacagaa ccaacaaaag
    10621 cggcgggcaa gcgcatcatt ccggtgaaat accacagcga caccaggtaa acaaaatagg
    10681 gaataatcga ccacatacta aaaatgatgg ttgcgcgtaa ggcttcaatg ccgcgttcgc
    10741 tggcaacaat ataatgcgcg ataagcgcaa aggtcgggaa aagtggaatc agcccggcga
    10801 tataataatt tttcgttttt gctaaaacac caatcaacag cactaccagc gcaccgagcg
    10861 cggctttgat taccagcccc atctttttac cttaacactt ccataacaag tcatcagtag
    10921 aatacctgat gaaaacttgt ttagaaacga ttgatagtaa gtaaaaacag cgcggtgtat
    10981 tgtgacgttt ttatatctac cgtgaatgtt atgaacacta tcgtatttgt ggaagatgat
    11041 gcggaagtcg gttcactgat tgccgcgtac ctggcaaaac atgatatgca ggttaccgta
    11101 gaaccgcgcg gcgaccaggc cgaagaaacc attttgcgag aaaatccgga tttggtgtta
    11161 ctcgacatca tgctaccagg caaggacggc atgaccattt gtcgtgattt acgcgcaaag
    11221 tggtctggac cgattgttct tctaacctct ctcgatagcg atatgaacca catcctggca
    11281 ctggaaatgg gtgcctgcga ctatattctc aaaacgacgc cccctgctgt tttgctagcg
    11341 cgtttacgtt tgcatttgcg tcagaatgag caagccacac tgaccaaagg tcttcaggaa
    11401 acgtctctga ctccctacaa agccctgcat ttcggcacgt taactatcga ccccatcaac
    11461 cgcgtagtca ccctggctaa cactgaaatc tcgctctcga cagctgattt cgaattattg
    11521 tgggaactag ctacgcatgc cgggcaaatc atggaccgcg atgcattgct gaaaaattta
    11581 cgcggcgtca gttatgacgg actggatcgt agcgtggacg tggctatttc gcggttaaga
    11641 aaaaaactgc tcgataacgc cgcagaacct tatcgcatta aaactgtgcg taacaaaggc
    11701 tatctttttg cgcctcatgc atgggatagt gacaataaac aatcagatga ctaatatttg
    11761 ttcatctgaa aaagagaaca attaa
//
LOCUS       DADMEQ010000117        11595 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.118, whole genome shotgun sequence.
ACCESSION   DADMEQ010000117 DADMEQ010000000
VERSION     DADMEQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.118"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            139..1824
                     /gene="btsS"
                     /locus_tag="JNZ05_003249"
     CDS             139..1824
                     /gene="btsS"
                     /locus_tag="JNZ05_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HAY8566374.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            1821..2540
                     /gene="yehT"
                     /locus_tag="JNZ05_003250"
     CDS             1821..2540
                     /gene="yehT"
                     /locus_tag="JNZ05_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HAY8566375.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            2587..3057
                     /locus_tag="JNZ05_003251"
     CDS             2587..3057
                     /locus_tag="JNZ05_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAY8566376.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(3098..3559)
                     /locus_tag="JNZ05_003252"
     CDS             complement(3098..3559)
                     /locus_tag="JNZ05_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HAY8566377.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(3684..5687)
                     /locus_tag="JNZ05_003253"
     CDS             complement(3684..5687)
                     /locus_tag="JNZ05_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HAY8566378.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQELVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLNAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(5684..6820)
                     /locus_tag="JNZ05_003254"
     CDS             complement(5684..6820)
                     /locus_tag="JNZ05_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY8566379.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(6813..8339)
                     /locus_tag="JNZ05_003255"
                     /pseudo
     CDS             complement(6813..8339)
                     /locus_tag="JNZ05_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111359.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8377..8724)
                     /locus_tag="JNZ05_003256"
     CDS             complement(8377..8724)
                     /locus_tag="JNZ05_003256"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566380.1"
                     /translation="MLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFAQLRGDSP
                     GDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVINTPELPSL
                     ADAVTGCYLTPLQ"
     gene            complement(8858..9093)
                     /locus_tag="JNZ05_003257"
                     /pseudo
     CDS             complement(8858..9093)
                     /locus_tag="JNZ05_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431464.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9104..10192)
                     /locus_tag="JNZ05_003258"
                     /pseudo
     CDS             complement(9104..10192)
                     /locus_tag="JNZ05_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
     gene            complement(10734..11141)
                     /locus_tag="JNZ05_003259"
     CDS             complement(10734..11141)
                     /locus_tag="JNZ05_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566381.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            complement(11202..>11595)
                     /locus_tag="JNZ05_003260"
     CDS             complement(11202..>11595)
                     /locus_tag="JNZ05_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HAY8566382.1"
                     /translation="RSEFAQLFADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKS
                     ATVGQLMGMRYKGWESGYEDAFVYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVY
                     RDNKSVTFAELDVFDLSEALSAPDVIFH"
BASE COUNT         2800 a         2943 c         3018 g         2834 t
ORIGIN      
        1 ctatagggtg attttatttt tgccaggcga ttttgagtga tcgtactcac gaattctcat
       61 ttttctgcaa gagttcaaag aaagttaaac gcaggcaatg tatgttacgc gttttaaagg
      121 gaagtgtggt ttgcgggtat gtacgatttt aatctggtgt tgctgctgct tcagcagatg
      181 tgcgtttttt tagtcattgc gtggttaatg agtaaaacgc cattattcat accgttaatg
      241 caggtcacgg ttcgtctgcc gcataaattt ctctgctaca tcgtcttttc catcttctgc
      301 atcatgggca cctggtttgg gttgcacatt gacgattcta ttgccaatac ccgtgcgata
      361 ggcgcggtaa tgggcggctt actcggcggt ccggtcgtcg gtgggctggt tggtctgacc
      421 ggcggcttac atcgatattc gatggggggc atgaccgcgc taagttgcat gatctcaacc
      481 atcgttgaag gattgctcgg cggcctggta cacagcatcc tgatccgtcg cgggcgcact
      541 gataaagtct ttaaccccat taccgccggt gccgtcacgt tcgtcgctga aatggtgcaa
      601 atgctgatca tccttgcgat cgcccgacct tatgaagatg cggtgcgtct ggtgagtaat
      661 attgctgcgc caatgatggt caccaatacc gtcggcgcgg cgctgtttat gcgtatattg
      721 ctcgataaac gcgcgatgtt tgaaaaatac acttcggctt tttctgccac tgcgctgaaa
      781 gtggcagcct cgacggaagg cattttgcga caggggttta acgaagtgaa cagcatgaaa
      841 gtggctcagg tgctgtatca ggagctggat attggtgcag tcgcgattac cgatcgagag
      901 aaattgctgg cctttaccgg aattggtgac gaccaccatt tacccggcaa accgatttct
      961 tcgacttaca ccttaaaagc gattgaaacc ggtgaagtgg tctacgctga tggcaacgaa
     1021 gtaccttatc gttgctcttt gcatccgcaa tgcaaactgg ggtcgactct ggtaattccg
     1081 ttgcgtggcg aaaatcagcg cgtgatgggc accatcaaat tgtatgaagc caaaaaccgt
     1141 ttattcagtt caataaaccg cacgctgggc gaggggattg cgcaactgct ttcggcgcag
     1201 attcttgccg ggcaatatga gcggcaaaaa gcgatgctca cccagtcaga aatcaaactg
     1261 cttcacgccc aggtgaatcc tcattttttg tttaatgcgc ttaacaccat taaagcggtg
     1321 atccgccgcg acagcgaaca ggccagccag ctggtgcagt atctttccac ttttttccgc
     1381 aaaaacttaa agcggccttc ggagtttgtt actctcgccg acgaaattga acatgtgaac
     1441 gcttatctgc aaattgaaaa ggcgcgcttc cagtcgcggt tgcaggtcaa cattgctatt
     1501 ccgcaagaat tatcccagca gcaattgccc gcgtttaccc tgcaaccgat agtggaaaac
     1561 gccattaaac atgggacatc acaactgctg gatacagggc gagtggcaat cagcgcccga
     1621 cgtgaggggc aacatttgat gctggagatc gaagacaatg ccggtttgta tcaaccggta
     1681 accaatgcca gtgggctggg gatgaatctg gtggataagc gtttacgtga acggtttggc
     1741 gatgactatg gaataagcgt cgcctgtgag cctgatagtt acacccgaat aacgttacga
     1801 ctaccatgga gggacgaggc atgattaaag tcttaattgt cgatgatgaa ccgttagcac
     1861 gggagaacct gcgtatattt ttgcaggagc agagcgatat tgaaatcgtt ggagagtgtt
     1921 caaacgccgt ggaagggatc ggcgcggtgc ataaactgcg cccggatgtg ctgtttctcg
     1981 atatccagat gccgcgcatc agtggtctgg aaatggtggg gatgcttgac ccggaacatc
     2041 gcccgtatat tgtttttctc actgcgtttg acgaatacgc tattaaagcc tttgaagaac
     2101 atgcctttga ttatctgctg aagccaattg atgaagcgcg actggagaaa acgctggcgc
     2161 gattgcgtca ggagcgcagc aagcaggatg tttcgctgtt accggaaaat caacaggcgc
     2221 tgaaatttat cccttgtacg gggcatagtc ggatttattt gctgcaaatg aaagatgtgg
     2281 catttgtcag cagtcggatg agcggtgtct acgttaccag ccacgaaggg aaagagggct
     2341 ttaccgaatt gacattacgt accctggaaa gtcgtacacc actactgcgc tgccatcgtc
     2401 agtatctggt taacctcgcg catttacagg agattcgtct ggaagataac ggccaggccg
     2461 agttgatttt gcgtaatggc ttaaccgtgc cggtcagccg ccgttatctg aaaagcttaa
     2521 aagaggcgat tggcctgtaa aagactgcta aaatggcttt ttgcctcatc aacacctgaa
     2581 ggcctcatga taagtaacga tattctgcgc agcgtgcgct acattttgaa agccaataat
     2641 aatgacctgg tgcgtattct ggcgctggat aatgtcgaag ccaccgcgga acagatcgcc
     2701 gtctggctac gtaaagaaga cgaagagggt tttcagcgtt gtccggacat tgttttgtcg
     2761 tcattcctca atggcctgat ttatgaaaaa cgcggcaagg atgagtctgc tccggcactg
     2821 gagccggaac gtcgcattaa taacaacatc gtgctgaaaa aattacgcat cgcgttttcg
     2881 ctgaaaaccg atgacattct ggctatcctc accgaacagc agttccgcgt ttcgatgccg
     2941 gaaattacag cgatgatgcg cgcaccggat cataaaaact tccgcgaatg cggcgatcaa
     3001 tttttacgtt attttctgcg tggactggca gcgcgccagc atgtgaagaa aagctaagac
     3061 gggtatggcg gccatgcgaa acatggccgc cgacagatta tttcacttct ttaaaaccag
     3121 cggctttcat caccagttcc atttgcgcca tagtgatacc ttttttggca tcttcagcag
     3181 aaacgttgat tcctgaaata ccctgcaggg ctttaaaatc cactttttcc atatcgatag
     3241 tcacgttttc ctgcgcgtag gtatcggtat aggttgattt ttcttcaaca cccgcgatgt
     3301 ttttgtattt ggcgcttaac ggctcaagtg tcttggcagc atcttctttg gtggttgcac
     3361 caatggaggc aaattgaatt ttggtttcag aagattgctt aagcaccttg tcacctttgt
     3421 agacataggt aatggcaatt tcagtgccgt tcagattggc gctgaatttc ttcgattctt
     3481 ctttgtcacc gcagccagca agagagaaaa ccagaacaga tgcaacaacg agggaaaaca
     3541 gcttattgaa agccttcatg taaaactcca ttttatttaa tcaagaaact ggtgactctc
     3601 accaggggct atataggata tgcctaatac cgtggcgtga gcagtccgga actggagtag
     3661 aactcttagt aaaaagcact atttcatcct tgttgctgaa gcatggggaa taattgttcg
     3721 caaagcaaaa caccgttatt cattgcttct acccgtgcct cgctttctgt attacgaaat
     3781 tgtgccaaca catgtgccag ccgataaaaa cccaccgcgg tgaggtcatt cgccagcaac
     3841 tctgcctgac taatagcgct ctgttcctga tagcgccagc cgttatggag cagttgaata
     3901 agtaacgcct ggcagcgcat cagcaactga tgagcagtag acggcacagg cagaacgctg
     3961 gcagaaggta gtgataccac cacaggcgca gtttctgcgt ccagcgccca ggcacgagtc
     4021 tttgtcatca ttacctgtgg ttccagtgtc aattgcccat caacaaaact gacaaagcca
     4081 gaaaccagac acacggggtc gtctgtttgt tgcaaaagcg ccgccatgcg ttcaatggca
     4141 taaggcgcgc tggctgaggc tggcagtgat aacgtcagca gattatcttc accttcgccg
     4201 ctgattacct gcgcatccag cgtctggcgg ctgctatccc aaccgagcga aatacactca
     4261 gcgaccggca gaataaataa gttatcgacc tgattaagag gccgtatgca ggcgggggga
     4321 cgctggcgta aatattcccg caaagccaca atgcccggct ggcgtaacgg cgcgttcaac
     4381 atttgccagg catcaggcga cagcggtaca acgctgctta agcggttgcg ggtagctaac
     4441 agcagctcgc catcggcact gcgttttgct gcttgtgaaa caatttgccc acccgccagt
     4501 gcgccagcct gaaaactaaa cagccgacgc gtagctgccg gtgagtcttc ctgttcactt
     4561 cgcggccaac tgcgcgaaag gtgcaaaata ctgccggtgt cgggatcggt aaaccagatg
     4621 cgtaaaccat aatgctcaat atcctgccag caacgcatac ctaaagacac cagccgcaga
     4681 tgatcaagct ttgcttctcc ggcaatgcca gagccaacga ccgtgcgcca cggcacagga
     4741 ggaacttcac caacactgtc gctccgggcc atctcttgtg cgcaatttaa tcgactgttt
     4801 aatgccgcaa gctgacgtaa gcattctccg gcatgatagt ggctggcgcg ggcgtggaag
     4861 gcatcaacgc tggcgcgcag ttgccgtagc gattcactca cccatcgcca gctgcagcgt
     4921 tccgctgctt gctgcgcgcg gctgaaagcg gcctcgtagt gaataagcgg ctggctgatg
     4981 ccgctcagcc ataatgcctg gcttagttgt tgaacatatt gacgacacgt tttgccttct
     5041 tcgctggcaa acggatcgtc agatgatgtg acgtgttcgc tgcgcatctg ccagattaaa
     5101 tgagtcaatt ccgcttgctg cgcttttgcc tggacaaagg cctgtactgc cagtacgaca
     5161 tgttcgcaaa gtgtgccttc aatacaatca caacgggcga aacgaatact gctgcgggaa
     5221 taaaaacgca catcgctcat cggtaagcgg gcagagggaa tttcacccgg cgcacagaac
     5281 aactcaatgg tgatgctttt agcgaccagc tcctgtgcgc gtttgcgggt ggcatcggga
     5341 agggtagcca gttcttccag ccagattgcc ggatcccatt cctcttcttt tcccgtaggc
     5401 tgggcggtag cacaaagtcg ttgataactt aacaccagca tcacgcgatg acggcacatg
     5461 ccgctggccc cacaggtgca ctgagcctct ttcagtgcct ggccgttcgc cagctgggta
     5521 cggacaccgt cactgaaggt ggcgattaaa gcgctgttct catggctgat ttccgggacg
     5581 ttgccatttt ccagttcctt aaggctgcgc ttaacaaaac cggcattgct taacgccgtc
     5641 agtgcctgcg gtgtcagttc taataattcc ggacgtagtg aattcatgac tgaagattct
     5701 ccgcaagcca tgatgccagc tcgcccggtg tcatggcggc tatttgtgcg ccgacattaa
     5761 ccagcgcctg ggccgtatcg cggtcatagc aaggtgttgc tgtgctatcg agcgctgcca
     5821 gtcccagcac tttgatgccg ctctggacac actttttcac ttggtgcgtc agcaatgatg
     5881 atgaaccccc ttcataaaaa tcgctcacga ggataatgac gctttttgca ggttgttcaa
     5941 taagttgccg accatactcc atggcactgg cgatattggt tccgccgcct aattgcactt
     6001 tcattaataa ctctaccgga tcggcaacgt ctgccgtgag atcaacgacg cttgtgtcaa
     6061 acgccaccag atgggtacga atgccgggta actgccataa acaggccgcc atcaccgcag
     6121 agtggatcac cgaatcgacc atcgatccgc tttgatcaac cagtaagacc agttgccatt
     6181 gctcgctttg gcgtttaatg cggctgttaa agcgggggaa ttcgatatac aacttgccgt
     6241 gttgcgggtg ccagtgttgc aggttggcgc gcagagtact tttgaaatca aagtttcgcg
     6301 ccagtggaat aaatgagcgg cgacgacgat cgcggacacc agaaaaagcc tgacgaactt
     6361 cctttgccag tcgcgccata atttcttcca caacctggcg cacaatcctg cgggcggcag
     6421 ccagtacttc ggggttcatc agatgtttgg tgtgcaaaac ggcgcgtagc aggctttcgg
     6481 aaggctgcat acgttccagc acgtcgagat ttgtcaccac atcttcaatg ccgtagcgca
     6541 gtacggcatc gctttccagc cgctcaatca cctgttgcgg aaacagcgtg tgaatactgt
     6601 tgatccactc aggagtggtg agatttgagc cacctaatcc actagagcgt tcaccacgct
     6661 ggagccgttc aggatcgcgc ccatacagcc actccagcgc gtgatctatc tgccgggcgt
     6721 tgtcatccag cccacaaagc gtcgtttctg ccgcttcgcc aagaattaat cgccagcgtt
     6781 gtagctcacg ggtggtcaga agatcgttca gttcagacat ggaaaactcc ccagtgttgc
     6841 agcgatgcca gtgcctgctg ttcgagttgt tgatgatgtg caatggcttg cggtggacaa
     6901 tgcaacggca tttgcagtgc cgaaacggga agttgcgcca gttgataatg ctcaagcacc
     6961 tgatgcgcca gcgtcccgcg ttctcgtggc ggtagccagg ccattgccgc gcgtaaatcg
     7021 ggcagggcat tggtaaaatc ggcatcactt agttgattta aatgactgct gaaaccggcg
     7081 ataaatgccg gctgacaggc cagttgatga cgggctaatg ccagcaaacc gtgcagcgcc
     7141 tcgccggatt gtgccgggga taactgcgcc agcatcgtca gcgccgcttc ggcactagcg
     7201 ttgggatgtt ccagacgcat cagcgcacca agagtcgcgc cgcgatccag tgccaggaca
     7261 tgaatcgcct gactgtgtcg ctccagcaag gctaccgccg cagaaagcga aacgccgggg
     7321 agttgcacac cgctatgtag atcgcgcagg atatgacaca acgcttgcca gctatgcaag
     7381 tgagcgtgaa actccttttc atccggtctg ccgttggatt cacacagcca cagcgtgcga
     7441 tcgatagccg cgcataacgt cgtttgtaat atctgcgcgc cttgcatacc gctaatttca
     7501 tccagccgcc ataaggcata tagcacttcc agcgcctggc ccatttcggc aaattgattt
     7561 tcctgggcga ttaataatgt gagttgctcc agtaattgtt gactgaagga cgccagaccc
     7621 gctgacgccg cctggctaag acatgtggta atgctgccga ttccacccgc gtccagcata
     7681 tcggcttcta atttatggcg tgcggcttcc tagagtgtgg caccaaagca ggcggcctca
     7741 atcaatgccg catgctggct aagcgggcgg gttaatttcc agcgttcttc accgttgcct
     7801 gcaagtgtca gtgtgcttcc ctgctggcgt acaatccctg ggatctccag tattgccagc
     7861 cgatgtaaca cctgactttg cgctagtcca ttggggttaa agcgatttaa ggtaagctcc
     7921 gccggaagag agattgcggt acgttccagt tcgcaagtga catcttttgg cagaggcggt
     7981 tgtggcgtag aaggggcaag tttaccgaat ccgtcacccg ctaatgtgtc tatcaacgtt
     8041 agcagaatcg gatcggtatc gggatgaata acgccgcgat agctccacgg caacggcgca
     8101 ttcagggctt ctttaatcag cgatcctgct atggcatcca gccagtcagt gcgtaacggt
     8161 agtgtatgac cgcgcaactg tgccagtgcc atcgcatgca gatgagcggc agccatatcc
     8221 gcggtcgaag caggcaactt gtgctggcgc aaacgggtga gaaccgtttt tagcagttgt
     8281 tctcccgcct gctgtaagcc ccactgccag caccagtttt gccagaccgg ggcaggcatt
     8341 cctgaaaggt atcctgccag cacatcaagg cgcttttcac tgtagggggg tgagataaca
     8401 acctgtaacg gcatctgcca gcgacggcaa ttctggcgtg ttaattacct gcggacattc
     8461 gcgccacatt tttgccagtg ccggagcgtg ccagccaccg cagacaacta acacgtcgcc
     8521 attattttgc tgcatcgccc atccaatcca gcgggccata aaggcttcgc gctgacgatt
     8581 gagagcatcg ccgggggagt cgccccgcag ttgggcaaaa tagtgcgcca gtgcagaggg
     8641 taatgcagtt tgctggcttt catcttcgaa caagtgatcc cacagggtgt cgctgttatc
     8701 catgcgggtg gcacgcagca gcaaggtctg gctttcctct tgcgtatcag gcgaatcgtc
     8761 ctcttcttca ctctgcgccc agcaaggcaa atcgatgaag taagtttgtg cctgaatacg
     8821 acgatcggct tgtagcgcct gccactccgg cgaaaattca gcaaatggcg tccaggcacc
     8881 acgcccgggg gctgcaccgt cctgatactg gcaataactg taaatagcta ccggaagctg
     8941 gtgggctaaa aacagttcgt ctacccggtc attaaaatca gccgggcctt caatcaacac
     9001 gtatcgtggc cgctggcttt cgattaacga tttcaccagc cgggcgcagg ccggactatg
     9061 atgccggatg ccgacaatta acggctcgct catgcatctt tcctcacggc aggcggtggc
     9121 gggcttgata ataagcctgc cagtgagctt ctttatgtgt ggcgacacgc tgttcgaagt
     9181 aacggcgcag acgagcgcga tcttcttcgt tatctttgac gatggttccg gcaatgcagt
     9241 ccaccaggtc ggctggctca cctgcgcgat tcgctaaaaa ccaggcgcgg acgcctacgg
     9301 cgtgggccac gttcacggct tcagcggtgg acataatcgc tgttagtgta tccattgacg
     9361 ttttcttttc gccgttggca cgcagatcgc ggaaggtgcg gaccagtaac tccagtaccg
     9421 cgtcaggcac tttatgcgga ataccgctat gggccagcaa acgcgccgaa gcactggcga
     9481 caagctccag ctcctgggcg aaatccataa tcggaaagac agtttcaaaa tcgaagcgac
     9541 gctttagcgc agcactcatt tcattgacgc cgcgatcgcg ggtattggca gtggcaataa
     9601 tattaaagcc ttctcgcgca tagagctgac tggcttcacc agtgagttcc ggcaccgtca
     9661 tcaccctgtc agaaagcata cctaacagac agtcttgcac ttccagcggt gtacgcgtta
     9721 tctcttcaaa acggacgatt ttgccgtcgc gcattccctg gtaaagcggg gcagggacca
     9781 gcgcttccgt tgatggacca tgattgataa gcagggcgta gttccagccg tacttgattt
     9841 gatcttcggt agtagatgcg ccgccctgga tggttaaccc ggcatcgccg ctaatagagg
     9901 ttgccagtaa ttcagacagg agagattttg cggtgctagg ttcccccacc aacatcaaac
     9961 cgcgtccggt ggcaagagtg actaacatac gttcgacagc ggagggacta atcaccactt
    10021 tacggctaat attttgcgcg ctatcgccaa ggataaaagc acgggccgca ggcaaactta
    10081 actaccaacc gggcgggcaa ggtgcgttat cattttgttt taattttgcc agttcatcgg
    10141 cgtataacac agcagcaggc ggacgctgca gatgattatt ctgcggtgac atatattcat
    10201 ccctgaaatg tattaatgaa ataaacgata ttttacgcgc aataaatgtt tggcgcatta
    10261 gttgtttgag cgaaaaatat ctggaaagca gatcacagta aataaaaaaa cctgctggtg
    10321 aggcaggttc ttttatttac tattttcagg cctaacacca ctgcctgtgc aacatcgtgt
    10381 tccaacgaac gaattataac cacctctgga cagtgttgca attaagataa tgagatttat
    10441 aatcctgact gttgggaagt tgcgccggat cgaacccatt tcaagtcctg gctaccggac
    10501 gggtggtgcc gcaggtagtg ctctgatccg gagtgcaaca tggcgactca acaaacgtaa
    10561 accagtatcg acaggcagtg taagggtaag gtgctggtgt tgtgctcctt aataaggaga
    10621 gcggatggac acgctgacac aaaagttaac cgtgctcatt gctgtactgg agctattagt
    10681 ggctctgtta cggttgattg atttgttgaa gtaacgggca aattgaaaca catctaaggg
    10741 ggaataccgt tccccccttt aataaagcta tactcgtcat acttcaagtt gcatgtgctg
    10801 cgacttgaat tatctagagt atataattct atttgttggt tgaatatatt ctgtagttta
    10861 caattaaaaa ttactattac aggttcaggt gattcgcctc gtaatttttg ctcatcaata
    10921 aactcatctc ttatataaat caacgcatca tttattagcg ggtcaacatc ctctcgatca
    10981 cgttgatgaa atgttggacg tagccggaac cgttccagtc ggtagacgga acctgtaaat
    11041 aattcagagc atttatggat ctcaagtctg aaggaaaacg gatcttcagg tacagggacc
    11101 tcataatcgt aaatagcaac cagctctttt tgcacgatca tcgctttccc cgctggtaag
    11161 tcgtgtagat aacgggcagt cgcatcactg cccgctttgt tttaatggaa aatgacgtca
    11221 ggtgcgctta acgcctcact caaatcaaac acatcaagtt cggcaaaagt gacggattta
    11281 ttgtcacggt acactcgaag agaacggaag ctcattagcg ctttgctatc aacattgtag
    11341 tggttaaaac cgggtgaaaa cttaaggacc agccggtact cacccaggtc atagacaaat
    11401 gcgtcctcat aacctgactc ccagcctttg tatcgcattc ccataagttg cccaacggta
    11461 gcggatttac cttcccagcg agtcaggctg ttactggttg actcgtcagg tgtgagcagc
    11521 accgtgcggc gcgacaactg gcgaaaaggc ggcataattt cgtaatcggc aaaaagctgt
    11581 gcaaattcac tgcgc
//
LOCUS       DADMEQ010000118        11574 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.119, whole genome shotgun sequence.
ACCESSION   DADMEQ010000118 DADMEQ010000000
VERSION     DADMEQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11574
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.119"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..436)
                     /locus_tag="JNZ05_003261"
     CDS             complement(<1..436)
                     /locus_tag="JNZ05_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8566383.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIDQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIE"
     gene            812..2452
                     /gene="trg"
                     /locus_tag="JNZ05_003262"
     CDS             812..2452
                     /gene="trg"
                     /locus_tag="JNZ05_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HAY8566384.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIGKIASGDLTMNDEPAGRNEIGRLSRHLQQIQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2490..3413)
                     /locus_tag="JNZ05_003263"
     CDS             complement(2490..3413)
                     /locus_tag="JNZ05_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8566385.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKESLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3630..4973
                     /locus_tag="JNZ05_003264"
                     /pseudo
     CDS             3630..4973
                     /locus_tag="JNZ05_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            5198..6853
                     /locus_tag="JNZ05_003265"
     CDS             5198..6853
                     /locus_tag="JNZ05_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HAY8566386.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6993..7217
                     /locus_tag="JNZ05_003266"
     CDS             6993..7217
                     /locus_tag="JNZ05_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HAY8566387.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            7280..7816
                     /gene="rimL"
                     /locus_tag="JNZ05_003267"
     CDS             7280..7816
                     /gene="rimL"
                     /locus_tag="JNZ05_003267"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HAY8566388.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7811..8791)
                     /locus_tag="JNZ05_003268"
     CDS             complement(7811..8791)
                     /locus_tag="JNZ05_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HAY8566389.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8915..9907
                     /gene="tehA"
                     /locus_tag="JNZ05_003269"
     CDS             8915..9907
                     /gene="tehA"
                     /locus_tag="JNZ05_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HAY8566390.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9904..10497
                     /gene="tehB"
                     /locus_tag="JNZ05_003270"
     CDS             9904..10497
                     /gene="tehB"
                     /locus_tag="JNZ05_003270"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HAY8566391.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10800..11468
                     /locus_tag="JNZ05_003271"
     CDS             10800..11468
                     /locus_tag="JNZ05_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HAY8566392.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(11486..>11574)
                     /locus_tag="JNZ05_003272"
     CDS             complement(11486..>11574)
                     /locus_tag="JNZ05_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAY8566393.1"
                     /translation="TPGQVENRALYPRPEGWGFTAHRIKSAI"
BASE COUNT         2978 a         2712 c         2898 g         2986 t
ORIGIN      
        1 cctcaatgcg tcggtctttt gacagctcca atgctgatgc cgctttttct ggatcaactg
       61 atattgcaat gtttcttttg gtgccaatct tgagcgcgcg taaaccagct tctccgcgct
      121 cttcatagac cttcagccac ctggctacag aaccactacc agcaagcata aagtgagcag
      181 cagcctgatt aagggacatg tgctggtcga tcacagcttt cacgacctta atacgcaact
      241 ctggatcagc actaacgcct ttaggtttgg gaattaaacc tttttctcca tgtttttcat
      301 agagggcaac ccatgtcctg acctgggttc gggggacacc aaaacgtgcc gagatgatcc
      361 tgtaaccatc atcagtagtg aagtagtgat tcacgacttc aaggcgcttt tcaaaagggt
      421 attttggctt tgacatatta ggggctattc catttcatcg tccaacaaaa tgggtgcagt
      481 acagttgata gaaatatcgg tcggggcctt cgtctttctg attcccggtt agcctgaaaa
      541 cagaaagtct caggcacccg caggcatcct atgaggtttc cttagggacg aaaataatca
      601 cttcacgaaa ttgcgtgctg ttttccagaa tttttcgtca ttcgggttag ccagtttagc
      661 cattcgttac tctcttcatt ccaatagcat taattttctc tgcaataatt gttgtaaaaa
      721 tgtgacgcaa agaggttttt ggtcataagt aattaccgtc aagtgccgat gactttctat
      781 caggagtaaa cctggacgag agacaacggt aatgaataca actccctcac agcgattagg
      841 ttttttgcat cacatcaggc tggttccgtt atttgcctgc attcttggcg gtatcttagt
      901 tctattcgca ttaagttctg ctctggctgg ctatttcctc tggcaggccg atcgcgatca
      961 gcgtgatgtt actgcggaga ttgagattcg gaccgggtta gcgaacagtt cagatttttt
     1021 gcgttcagcc cggatcaata tgattcaggc cggggctgcg agtcgtattg cggaaatgga
     1081 agcaatgaag cgaaatattg cgcaagccga atcggagatt aaacagtcgc agcaaggtta
     1141 tcgtgcttat cagaatcgac cggtgaaaac acctgctgat gaagccctcg acactgaatt
     1201 aaatcaacgc tttcaggctt atatcacggg tatgcaacct atgttgaaat atgccaaaaa
     1261 tggcatgttt gaagcgatta tcaatcatga aagtgagcag atccgaccgc tggataatgc
     1321 ttataccgat attttgaaca aagccgttaa gatacgtagc accagagcca accaactggc
     1381 ggaactggcc catcagcgca cccgcctggg tgggatgttc atgattggcg cgtttgtgct
     1441 tgccctggtc atgacgctga taacatttat ggtgctacgt cggatcgtca ttcgtccact
     1501 gcaacatgcc gcacaacgga ttggaaaaat cgccagtggc gatctgacga tgaatgatga
     1561 accggcgggc cgtaatgaaa tcggtcgctt aagtcgtcat ttacagcaaa tacagcattc
     1621 actggggatg acagtaggga ctgttcgaca gggcgcggaa gagatttatc gtggcaccag
     1681 cgaaatttca gctggcaatg cggacctgtc atctcgcacc gaagaacaag cggcggctat
     1741 cgaacaaact gccgccagca tggagcaact cactgcgacg gtgaaacaga atgcggataa
     1801 cgcgcatcat gccagcaaac tggcgcaaga ggcttctatt aaagccagcg atggcgggca
     1861 gacggtttcc ggtgtagtaa aaacgatggg cgctatctcc acgagttcga agaaaatttc
     1921 tgagatcacc gccgtcatca acagtattgc tttccagacg aatattctgg cactgaatgc
     1981 tgccgttgaa gccgcgcgag cgggtgagca agggcgtgga tttgccgttg tcgccagcga
     2041 agtacggaca ctcgcaagcc gcagcgctca ggcagcgaaa gagattgaag gcttgatcag
     2101 tgaatcagtc aggttaattg acctggggtc ggatgaggtg gcaacggcag ggaaaaccat
     2161 gagcactatt gttgatgccg tcgcgagtgt cacacatatc atgcaggaaa tcgccgccgc
     2221 ctcggatgaa caaagtagag gcataactca ggttagccag gcgatttctg aaatggataa
     2281 ggtgacgcaa cagaatgctt ctctggtaga agaggcctca gcagcggcgg tgtcccttga
     2341 agaacaggct gcacgattaa ctgaggcggt gaacgtattc cgtctgaaca aacattctgt
     2401 gtcggcagaa cctcgcggag cgggtgaacc agttagtttc gctacggtgt gaaaatgttc
     2461 aaggagggat cgacagatcc cttcaccttt cagaacggca ttgattttcg aatagcgtta
     2521 atcatcaact ggcaaccaga agagaacgtc gcatctacgc gggtcagtat tccaatcggt
     2581 tcgcctgcac catgtcccgg aacaggcagg gccaccagcg tggcatgacg caggtcgtct
     2641 tttacagcgc cagaagggac aaaccacacg taatcgtatt caacggtaag ttgacgagat
     2701 agcgaagcag acagcgtttc gatacaaccc gaaggaattt tacagccctg gctctgcact
     2761 aatgcatctg aatgctggcg tggcgcagtg ccttctggtg atacaacgac cggccattcc
     2821 agcacccggc ttagcgttac gttctcctga agtagcgggt gattagggcg gacaaccagc
     2881 ttcaacgatt caagaaacag cagttcgtaa ttaagcccgg tcatcagttc aggatctgac
     2941 atccgaccaa tgccgatatc gatttccccg gtttttaaac ccgccagaat catagggtta
     3001 ctcattgtcg caacttgcaa ggtcgtctct ttttgttgct gatgaaactg acctataacc
     3061 gaaggtaata tccccagtgc cgcagtaggt agtgcaccaa ccctgacgac atcattatta
     3121 agactttctt tacgatgaag cgactgtccg gcagtgttga tggcgtcaag aactctgact
     3181 gcatgcgtta aaaattgttc gccgggtaag gtaagttgcg ccccctgacg accacgctca
     3241 aacaagcgag cgcccgtcag ctgctccagt tcattcaatg tattagagag cgcaggttga
     3301 ctcaaattaa gggtttcagc tgcgcgcccc aaagttcctt gttgtgcgac agctacgaat
     3361 gtatgaaggt ggcgcaaacg gatgcgctga ctaaacagac tatttttttc cataagcgat
     3421 gttaaaaacg aagcggtgtc gctgacaagt gaagttgttt gattatgata acttgattgt
     3481 aaaatattat taacaattaa agcaattatg ttacagcaaa atggataata ttgatgtttt
     3541 cgcggcgaga tcacagtttg taaattcttc ccgccagagt gaatgctgtt acctacactc
     3601 cagattactg accactggag gcagacacta tggcgaacag catcacggcg gatgagattc
     3661 gggaacagtt ttcgcaggca atgtcagcca tgtaccagca agaagttccg caatatggca
     3721 cgctgctgga actggtagct gatgtgaatc tggctgtgct ggaaaacaat cctcaactgc
     3781 acgaaaaaat ggtaaatgca gacgagctgg cgcgactgaa tgttgaacgt catggggcga
     3841 ttcgcgttgg gactgcacaa gagcttgcta ctcttcggcg gatgtttgcc attatgggga
     3901 tgtacccggt gagctattac gatctctcgc aggcaggggt gccggtacat tcgacagcat
     3961 ttcggcccat tgatgatgct tctctggcgc gtaatccctt ccgcgttttt acatccttac
     4021 tccgccttga gcttatcgag aacgaaattt tgcgccagaa agcggcggag attctgcgtc
     4081 agcgcgatat cttcacccca cgttgtcgac aactgttaga ggaatatgag cagcagggcg
     4141 gttttaacga aacacaggca caggagtttg tgcaggaagc cctggaaacg tttcgctggc
     4201 actagttagc aacggtagat gaagaaacct atcgcgcctt gcacaacgaa catcggttga
     4261 ttgctgatgt ggtctgtttt cctggatgcc atatcaacca cctgacgcca cgtacgctgg
     4321 atattgaccg ggtgcagtcg atgatgcctg aatgcggaat tgaacccaaa attctcatcg
     4381 aggggccgcc gcgccgcgag gtaccgattt tactacgcca gaccagcttt aaagcactgg
     4441 aagagacggt gttgtttgcg gggcagaaac agggcacgca taccgcgcgc tttggtgaaa
     4501 ttgagcagcg tggcgtggca ttaacgccga aagggcgaca actgtatgat gatcttctgc
     4561 gtaacgctgg aaccgggcag gataatctca ctcaccaaat gcatttacag gaaaccttcc
     4621 gcacttttcc tgacagtgag tttttaatgc gtcagcaagg gttggcatgg ttccggtacc
     4681 gtctgacgcc ttcgggtgag gcgcatcgtc aggcgattca tcctggagac gatccacagc
     4741 ccttaattga acgtggttgg gtagtggcgc aacccatcac ctatgaagat ttcttgcccg
     4801 ttagcgcggc ggggatcttc cagtcaaatc tgggtaatga aacgcagaca cgcagtcacg
     4861 gtaatgccag tcgcgaagca tttgagcagg cgttgggttg tccggttttg gatgagttcc
     4921 agctttacca agaagcggaa gaacgcagta aacgtcgctg tggtttgctt taaattctga
     4981 ccatccgcct ttgcaaaaat ttgcctgatt ttacaaacga atcaggctca tcccatcgac
     5041 ataaaaaaaa tgccgattta tgcatattct ctcagttcaa caattggatt attaataaat
     5101 attgtctaga gtgagcggtc ataaataagc actttcttgc cgctgaaaac gaccagcgcg
     5161 ggaccattca caacaccaga aggactcact ttcaggtatg gatcgtagac gatttattaa
     5221 aggttcaatg gctatggccg ccgtgtgcgg taccagcggc attgcttctc ttttttctca
     5281 ggcagcattc gcggcagatt ctgatattgc cgacgggcaa acccagcgtt ttgacttctc
     5341 cattctacag tcaatggcgc acgacttagc gcaaacagcg tggcgtggtg cgcctcgtcc
     5401 gttacctgac acgctggcga caatgacgcc gcaggcttat aacagtattc aatacgacgc
     5461 cgaaaaatcg ctctggcata acgttgagaa ccgtcaactg gacgctcagt tcttccatat
     5521 gggaatggga ttccgtcgcc gcgttcgtat gttttctgta gatccagcaa cacatctggc
     5581 gcgtgaaatt cactttcgcc cggagttgtt caaatacaac gatgcaggtg ttgataccaa
     5641 acaattagaa gggcaaagcg atctcggctt tgccggtttt cgcgtgttta aagcccccga
     5701 actggcgcgc cgtgatgtag tatcatttct cggcgcgagt tatttccgcg ccgttgatga
     5761 tacatatcaa tacggtttgt cggcccgcgg cctggcgatc gacacttaca ccgacagtaa
     5821 agaagagttc cccgacttta ccgccttctg gtttgatacg gtaaaaccgg gggcaactac
     5881 ctttaccgtt tatgcgttgc tcgatagcgc cagcattact ggtgcctata agttcactat
     5941 ccattgtgag aaaagtcagg tgattatgga tgtggaaaat cacctgtatg cgcgcaaaga
     6001 cattaaacag ctgggcattt cgccgatgac cagtatgttc agctgcggta ctaatgaacg
     6061 tcggatgtgc gatacaattc atccgcaaat tcatgactct gatcgtctgt ccatgtggcg
     6121 gggcaacggc gagtggattt gccgtccgct gaataatccg caaaaattgc agttcaatgc
     6181 ttacaccgac aacaacccga aagggtttgg tttattgcaa ctggatcgtg acttctccca
     6241 ttatcaggac attatgggct ggtataacaa acgtccaagc ctgtgggtgg aaccgcgtaa
     6301 caagtggggt aagggcacca tcggcctgat ggaaatccca acaacgggcg aaacgctgga
     6361 taacattgtc tgcttctggc agccagaaaa agctgtaaaa gcaggtgatg agtttgcatt
     6421 ccagtatcgt ctgtactgga gtgcgcaacc gcctgttcat tgcccattag cgcgcgttat
     6481 ggcgacgcgt accggcatgg gcggtttccc ggaaggttgg gcgccaggtg aacactatcc
     6541 cgaaaaatgg gcgcgtcgtt ttgccgtcga tttcgttggt ggtgatctga aagctgccgc
     6601 gccaaaaggc attgagccgg tgattacgct ttccagtggg gaagcgaagc aaatcgaaat
     6661 tctctatatt gaacccatcg atggttatcg tattcagttt gactggtatc cgacttcgga
     6721 ctccactgat ccggtcgata tgcggatgta tctacgttgt cagggggacg ctatcagtga
     6781 aacatggctg tatcagtatt tcccgccagc gccggataaa cgtcagtatg ttgacgaccg
     6841 cgtgatgagt taatcgtttt ttcttcggca ccttcttcgg gaggtgccgt ctggttaaac
     6901 acgatcccgc tcgcattttt ccctaagtta aatgagtaat ctgatggtgt gtatttcaga
     6961 tacaccgtgt cagccactaa cagggagtgc gtatgtttcc agaataccga gatttaatat
     7021 cccgtctgaa aaacgaaaat cctcgcttta tgtccttgtt cgataaacac aataaacttg
     7081 atcatgaaat tgccagaaag gaaggttccg acggtcgagg gtacaatgcg gaagtggtcc
     7141 gcatgaaaaa acaaaagcta cagttaaaag atgagatgct caaaatcctg cagcaggaga
     7201 gcgtcaaaga ggtgtaaact ttcctaagcc gccagccagg cggctttttt aacaacagca
     7261 tggattgact ggagataaga tgactgaaac gataaaagta agcgaatcac ttgaattaca
     7321 tgctgttgcg gaaagtcacg tcacacctct ttatcagtta atctgtaaaa ataaaacctg
     7381 gttacagcag tcgctaaact ggccgcagtt cgttcagagt gaagaggaca cgcgaaaaac
     7441 ggtgcagggt aatgtgatgt tgcatcaacg cggctatgcc aaaatgttca tgattttcaa
     7501 agaagatgaa cttatcggcg ttatctcgtt taatcgtatt gaaccactga ataaaactgc
     7561 tgaaatcggc tactggctgg acgaatctca tcaggggcag gggatcattt ctcaggcgct
     7621 gcaggcattg attcatcatt acgctcagtc tggtgaactt agacgcttcg tgatcaaatg
     7681 tcgggtggac aatccgcaaa gcaaccaggt cgctttgcgc aatggtttta tccttgaagg
     7741 ttgcctgaaa caggctgagt tcctgaatga tgcctatgat gatgtgaact tatacgcgcg
     7801 tattatcgat tcataataac cctgccagcg gcgttcgcgt aatgcgctcc tcgccgttaa
     7861 tgacttttgg accacggaca tgaaccgtgt cgccatcaaa ggcttcaacg acggcgtggt
     7921 cggttaactc cacatggttt tcaattatca ccgcacctgt aatacggctc tcaccttgta
     7981 ttacaacgtg ctcgtcgagc agaatcggtc caccacgtac cacggcattt ccgccaatca
     8041 agacatgatg ttttaacaca caattacctt ccacaatggc atattccgcc acctgcgaac
     8101 tgtaatgaat cgtcggaatg gcatcttctt ctatgccagc tttcacctgc gcgtgaccgt
     8161 agactttagc gcaatcgcat agccagacgt tgttctcttc attaccttca acactggcaa
     8221 aatcaaaaac ttcggcgcga tgttcaataa aagcataccg gacgacggca tcgccataaa
     8281 tttgtgcctg gtggacaata cgcgaggcgc taacccttgc gcgatcataa atttggagca
     8341 ggagttgatg gtcgggcgtt aagccttgtg cggcgacgat catagaatgt tgatcaatta
     8401 aggcgtgacc aaatattcta cattgcccgt aaactaatga gtcatgaatg gtgacactgt
     8461 cacttatata tgcaccctgg ctgatttcac tattatcgat ccagacgtta tccgttgcgt
     8521 aaacctctcc ccacagcacg ctggtgccgg tgatacgcgt attaccggaa atcaccgttc
     8581 cgccgaacgc aatagcattc tggtcgtaaa tccagcaatt tccttcctgt gctaacaccg
     8641 tttcgcgatc gatccagccg ccagcggttc ctgctatcac atcgttaaaa tcgctaatgg
     8701 cgataatctg ccgtaataac acactttttt tagtgccatc ttcctgataa ctaaaggctc
     8761 gctgttcttc acttaggcga tatttgcgca tagcgttttc ccacaggtga cttactataa
     8821 ccgtagcaaa ttctgcggct ctggctatgc tcgagaaaat tccataaaat gcatttcaaa
     8881 tatactttat aaattaaaca aaatgagtaa gaagatgcag agcgataaag tgctcaattt
     8941 gccggcaggc tactttggta ttgtgttggg gacgataggg atgggatttg cctggcgcta
     9001 tgccagccag gtttggcagg tcagccactg gttaggggat gggctggtga ttctggcgat
     9061 gatcatctgg ggattattga ctagcgcatt tattacccga ctcatacgct ttccgcatag
     9121 cgtgctggcg gaagttcgcc atccagtgct gagcagtttt gtgagtttgt ttccggcaac
     9181 gacgatgctg gtggcgattg gttttgttcc gtggtttcgc ccactggcgg tgtgcatgtt
     9241 cagttttggt gtcgtggttc agttggctta tgccgcctgg caaactgcgg gattatggcg
     9301 cggatctcac cctgaggaag ctaccacgcc tggactgtat ctgccgacag ttgccaacaa
     9361 ctttatcagc gcaatggcct gtggtgcgtt gggctacacc gacgccggtc tggtgttttt
     9421 aggcgcaggc gttttctcat ggctaagcct tgaaccggtg atcttgcagc gtctgcgcag
     9481 ttcgggagaa ttacccacgg cactgcggac atcactcggc attcagctcg ctcctgcgct
     9541 ggtggcctgt agtgcctggc tgagcgtcaa cggcggcgag ggtgacacgc tggcgaaaat
     9601 gcttttcggt tatggactgc tgcaactgct gtttatgcta cgtctgatgc catggtatct
     9661 ctcccagcca tttaatgctt cattctggag tttctcgttc ggcgtatctg cactggcaac
     9721 caccggtttg catctgggga gtggcagcga taatggattt ttccatacgc tggcggtgcc
     9781 gctgtttatc tttaccaatt ttattattgc aatactgctc atccgtactt ttgcgcttct
     9841 gatgcaggga aaattgttag tcagaaccga gcgcgccgtt ttaatgaaag cagaggacaa
     9901 agaatgatca ttcgtgacga aaactatttt actgataaat atgaattaac ccgcacacac
     9961 tctgaagtac tggaagcggt gaaagtggtt aaaccgggta aaacgctgga tctgggctgt
    10021 ggcaatggtc gtaacagtct ttacctggca gccaatggtt atgatgttga cgcatgggat
    10081 aaaaatgcca tgagtatcgc caacgtcgag cgcattaaat ccattgaaaa tctggataat
    10141 ttacacaccc gggtcgttga tctgaataac ctcacatttg atggacagta cgattttatt
    10201 ctttcgactg tggtgctgat gttccttgag gctaaaacca tcccagggtt gattgccaat
    10261 atgcaacgtt gcactaaacc tggtggttac aacttgattg tggcggcgat ggataccgct
    10321 gattatccat gtaccgtcgg cttcacgttt gccttcaaag agggagaatt acgtcgatat
    10381 tacgaaggct gggagagggt gaaatacaat gaagacgtcg gcgagctgca ccgcaccgac
    10441 gccaacggta atcgtattaa actgcgtttc gccacgatgc tggcacgtaa aaaatgaccc
    10501 ggtaagcaca aaacgcgtga aaattcccca cgctgagatg atttactgtt cttcttttcg
    10561 gtaagcatat tttttatcga agggatgtga aattaatcac agtagtcgaa gttttttagc
    10621 agcttaactt actgaaattt aactactgat gattgactta gccccttttt cggcattgac
    10681 tatgtcgtct gaaaaggggc tgaaaaattt attttcacca acactttttt tgccacaaca
    10741 cgaagcggcg ctttttgcta taacttagaa agtaatataa tcatctcagg aaactattca
    10801 tgcgtaccac atcatttgcg aaagttgcag ctttatgcgg cttgttggct ctgtctggtt
    10861 gtgcctctaa aatcacccag ccagataaat attctggttt tttaaacaat tactctgatt
    10921 taaaagaaac aacctcagca acaggtaaac ctgttttacg ttgggtagac ccgagttttg
    10981 atcaaagcaa atatgacagc atcgtctgga acccaatcac ttattatccg gtaccaaaac
    11041 cttctaccca ggtagggcag aaagttctgg ataaaatttt gaactatacc aacaccgaaa
    11101 tgaaagaagc gatagcgcag cgtaaaccag tggttaccac cgctgggccg cgtagtctga
    11161 ttttccgtgg ggccattacc ggtgtagata ccagcaaaga agggctgcaa ttctatgaag
    11221 tggttcctgt tgcattagtg gttgcgggga cgcaaatggc tacaggccac cgtaccatgg
    11281 atactcgcct ctattttgaa ggtgagctga ttgatgcagc gactaataaa ccggttatca
    11341 aagtcgttcg tcagggcgaa ggtaaagacc tgaataacga aagtacgcca atggctttcg
    11401 aaaatattaa acaagttatt gatgacatgg cgaccgatgc caccatgttt gacgttaaca
    11461 aaaagtagtt ccagacgcgc catcgttaga tggcgctttt tatccggtgc gccgtaaaac
    11521 cccatccttc agggcgggga tataaggcgc ggttttccac ctgaccaggt gttt
//
LOCUS       DADMEQ010000119        11555 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.120, whole genome shotgun sequence.
ACCESSION   DADMEQ010000119 DADMEQ010000000
VERSION     DADMEQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.120"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="JNZ05_003273"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="JNZ05_003273"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1564)
                     /gene="garK"
                     /locus_tag="JNZ05_003274"
     CDS             complement(413..1564)
                     /gene="garK"
                     /locus_tag="JNZ05_003274"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HAY8566394.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGAGSLTDDVGV
                     VHQHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1661..2551)
                     /gene="garR"
                     /locus_tag="JNZ05_003275"
     CDS             complement(1661..2551)
                     /gene="garR"
                     /locus_tag="JNZ05_003275"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAY8566395.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2581..3351)
                     /gene="garL"
                     /locus_tag="JNZ05_003276"
     CDS             complement(2581..3351)
                     /gene="garL"
                     /locus_tag="JNZ05_003276"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HAY8566396.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATHKLADTFKK"
     gene            complement(3367..4701)
                     /locus_tag="JNZ05_003277"
     CDS             complement(3367..4701)
                     /locus_tag="JNZ05_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8566397.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDSAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5076..6647
                     /gene="garD"
                     /locus_tag="JNZ05_003278"
     CDS             5076..6647
                     /gene="garD"
                     /locus_tag="JNZ05_003278"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HAY8566398.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPTSELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6699..7523)
                     /gene="agaR"
                     /locus_tag="JNZ05_003279"
     CDS             complement(6699..7523)
                     /gene="agaR"
                     /locus_tag="JNZ05_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HAY8566399.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7772..8572
                     /locus_tag="JNZ05_003280"
                     /pseudo
     CDS             7772..8572
                     /locus_tag="JNZ05_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="with KbaY catalyzes the formation of
                     dihydroxyacetone phosphate and
                     D-glyceraldehyde-3-phosphate from
                     tagatose-1,6-bisphosphate; subunit Z may act as a
                     chaperone for the Y subunit; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
     gene            8572..9036
                     /gene="agaV"
                     /locus_tag="JNZ05_003281"
     CDS             8572..9036
                     /gene="agaV"
                     /locus_tag="JNZ05_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HAY8566400.1"
                     /translation="MVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQQNL
                     MEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRINVG
                     NMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9047..9826
                     /locus_tag="JNZ05_003282"
     CDS             9047..9826
                     /locus_tag="JNZ05_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HAY8566401.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGTEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            9816..10625
                     /locus_tag="JNZ05_003283"
     CDS             9816..10625
                     /locus_tag="JNZ05_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAY8566402.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            10712..11146
                     /locus_tag="JNZ05_003284"
     CDS             10712..11146
                     /locus_tag="JNZ05_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY8566403.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEEYPVEEGI"
     gene            11143..>11555
                     /locus_tag="JNZ05_003285"
     CDS             11143..>11555
                     /locus_tag="JNZ05_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY8566404.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         2755 a         3101 c         2949 g         2750 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccgccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcacccgcaa
      601 tgccaatcac cggtttatgg tacttcttcg ccacgtttgc gacgccaatc ggtaccttgc
      661 cgtgaatact ctggctgtca atacgccctt caccggtgat caccagcgta caatcgtgaa
      721 tatgttcctc cagattcagc gccgtagtga cgatttcaat accacttttc agttccgcac
      781 caagaaacgc catcagcgcc gcacccatac cgcccgcagc tcctgcgccg gggacatctt
      841 ttacatcaac atgcagcgct tttttaatga catcggcata gtgagagagg ttattgtcca
      901 gctcaacaat catcgcttca ctggctccct tttgtgggcc aaagatgcgc gatgcgccgt
      961 tatcgcccac cagcggattg gtgacatcac aagcgacgcg aatgacgcaa tcttttaagc
     1021 gcggatcgag gccggaaata tcaatatcat tcagagtatt aagactaccg ccgccaaaac
     1081 caatttcatt gccgttggcg tcgcataatt tcgcccccag cgcctgtacc atgcctgcgc
     1141 cgccatcatt tgtagcgcta ccgccaatgc cgataataat gtttgtcgcg ccgctctcca
     1201 gcgcctgcag gattaactcg cctgtgccgc gtgaagtggt cacgagtgga tcgcgttttt
     1261 ccgcaggtac cagctccagc ccactggccg ccgccatttc aataaacgcg gttttgccat
     1321 cgccggagat cccccaactg gcattcactt tctcgcccag cggccctgta acccaggcgt
     1381 gacgttcaga accctgggtg gctgcaatca tcgcttccac cgttccttcg ccaccgtcgg
     1441 caaccggaat agaaacgtac tgtgcatcag gaaaaatttc ccgaaatcct ttttctatcg
     1501 cctgcgcaac ctcgctggca gataaacttt ctttataaga gtctggggcg attacgattt
     1561 tcatacctat gcctgttacc acatgacgcc ggagggcgtt tctcttattc ggcctggatt
     1621 ccaggcccgg attgcaatac gccatccggg cacgacgtca ttaacgagta acttcgactt
     1681 tcgccagttt ttcgtagtag cacgccaggg cgctatgatc cgccgttcct aaaccatctg
     1741 ctcgcagtgc ctgcatcatc tccataaccg cagctgtgag cggcagttgc gcgccgacgc
     1801 cgtgggaagt atccagcgca tttgccagat ccttaatatg cagatcaata cggaagcccg
     1861 gcttgaagtt gcggtccatc accatcggcg ctttggcatc cagcacggta ctgcccgcca
     1921 gtccaccgcg aattgcctga tacaccaggt ccgggttaac gcccgcttta gttgccagcg
     1981 ttaacgcttc tgacatcgcg gcaatattca gcgccacaat gacctgattc gccagtttgg
     2041 tgacgttacc tgcaccaatt gccccggtat gcaccacgga acccgccatt gctttcatca
     2101 aatcatagta tttgtcgaaa atagccttgt cgccgcccac catcactgac agcgtaccgt
     2161 cgatggcttt cggttcaccg ccgctcaccg gagcatccag catatcaatg cctttcgctt
     2221 tcagcgcttc gctgatttca cggcttgcca gcggtgcgat agaactcata tcgatcaata
     2281 ccgtacctgg cttcgcgcct tcaataatgc cattctcacc cagcgccacc tctttcacat
     2341 gaggggagtt tggcagcatg gttatgatga cgtcgcactg ttcagcgatc gctttagccg
     2401 tagaggctgt ttctgcacct gcagcaatca cgtcagcaat agcttctggg ttacggtcag
     2461 caaccaccag cgagtaacct gctttcagaa ggtttttact cattggttta cccataatcc
     2521 ccaggccaat aaaaccaact ttcatagtca tatcaatcat ctctcttgtt gcggtggtgg
     2581 ttatttttta aaggtatcag ccagtttatg agtggcagag cggaagacgc cgagatcgct
     2641 gccgacagcc acaaacgtcg cgccccattc cagataacga cgcgcatcgg cttcgaccgg
     2701 cgcgaggata ccgctgggtt tgccgtgcgc gctggcacgg ttaaaaatgt gctgaattgc
     2761 tttttgtaca tccgggtgtg atgcattgcc gagatggcct aatgccgcgg ccagatcgct
     2821 ggggccgacg aagatgccgt ctacgccttc ggtagcggca atggcatcga cgttatctac
     2881 gccctgctga ctttctatct ggaccagaat agtgatgttc ttgttcgact gagcgaaata
     2941 atccgccacg gtgccaaaca tattggcgcg gtgagaaacg gagacgccgc gaatgccttc
     3001 cggtgggtaa cgggttgatg ccaccgcctg ctctgcttcc tcttttgttt ctacaaaagg
     3061 aatcaggaag ttatagaaac cgatatccag aagacgctta ataattaccg gctcgttggt
     3121 cggcactcgc actactggcg cgctggcgct gcctttcaag gccattaact gcggaataaa
     3181 cgtggagata tcgtttggcg catgttcgcc atccagcacc agccagtcaa acccagccaa
     3241 accaagaact tcagtgctaa tcgggttaga gagtgctgac cagcaaccaa tttgtacctg
     3301 tttcgcagcc agtgcggctt tgaatttatt cgggaaaaca tcgttattca tcgcttatac
     3361 ctttgcttat ttctgcaatt ccatacgttt aatgtcgcca actacgaaga ggtagcagac
     3421 catcgccatc agcgctgaac atcccacgaa aaccagtgct gcattgaagg agtgcagttc
     3481 acttaccagg tagccaatca ccagtggagt gacaatggag gcaacattgc caaagacgtt
     3541 aaagacgccg ccacagaggc caacaatctc tttcggcgcg ctgtcagaaa tcaccggcca
     3601 gcccagagca ccaaatcctt tgccaaaaaa agccagcgcc atcagcatga ccaccagcgt
     3661 ggtgttgttg gtgtagttac ataagatgat ggtggaagcc agcaacattc ccagcacaat
     3721 cggtagctta cgtgccaggg tcagggataa accgcgtttg atcagataat ccgagaagac
     3781 acctcccagc acgccgcccg caaaaccaca cagtgccgga atcgaggcga ccagacccac
     3841 tttcagaatc gacatgcctt tttcctgcac cagataaatc gggaaccagg tgaggaagaa
     3901 ccaggtgatg gtgttgataa aatattgtcc gaaaaatacg cccaacatca tgcggttaga
     3961 gagcaattgc ttgatgtaat gcagtttggg accgcttgct gccgcgctgc ccggcttttt
     4021 gtggtccata tcgaccaccg cgccattttc agagataaac ttcagctctt cctcagacat
     4081 acgtgggtga tctgtcgggt tatgaatcaa cttgatccac agcgccgtca gcacaaaacc
     4141 aatcaccccc ataacggtaa agacgtgctc ccatccccag gcgaaagtca gccagccaag
     4201 cagaggcgaa aagagcgcca gcgagaaata ttgtgccgag ttaaagatgg cggaggcagt
     4261 accacgttct ttcgtcggga accaggcggc gacaatacgg gcgttcgccg ggaatgatgg
     4321 agcttccgag aatcccagca taaagcgcat aaagaacatg gagatccctg cccaggccag
     4381 cgggaacata tcaacaaagc cttgcaggaa ggtgaacagc gaccagaaaa agaggctgta
     4441 ggtgtaaact tttttcgagc caaacttatc aagcaaccag ccgccgggga tttgcatcag
     4501 caagtaggcc cagccaaaag cggagaagat gtaacccatc gaaaccgcac ttaactgcaa
     4561 ctcttttgcc acttcggtac cagcaataga cagcgttgca cgatcggcgt agttaacggc
     4621 ggtaacaata aaaataatca gtaatattaa atagcgggta tgcacacctt tccttttttc
     4681 gtcaacggtg tccagaatca ttttatttac ctcgggtact tatgctgatt tttattatta
     4741 tggggaaggt gttatttata agtttcattt atgccgtaac gacaatgaac tcgggaatta
     4801 gtataagcag cgcgagaata ataatcattg tgcaaatgct aatttaatta atacttttta
     4861 aatattattt tgagcatatg cacataaggt tgcgcgctaa agcacagatt tgcgctttac
     4921 cttgccgggc ggcactgcaa tccctgaaat gattgacatt gatcacattt ctgcgtttaa
     4981 actcctgaca ttcttatttc acccaatgaa gtcatttatt tttaaatgag accaggtcct
     5041 cattttaata acccctggct ggagaatatt gcacaatggc caacatcgaa atcagacaag
     5101 aaacgccaac tgcgttttat ataaaagttc acgacacaga taatgtggca attattgtta
     5161 atgataatgg cctgaaagca ggaacgcgtt ttccggatgg gctggaatta attgaacata
     5221 ttccccaggg gcataaagtc gcattgctgg acattccggc taatggtgaa attattcgtt
     5281 atggcgaagt gattggttac gccgtgcgtg caatcccacg cggaagctgg atcgacgaat
     5341 caatggttgt actaccggaa gcgccgccgt tacacacgct gccactggca accaaagtcc
     5401 cggaaccctt accgccgctg gaaggataca cctttgaggg ctatcgcaat gccgatggca
     5461 gcgtgggcac caaaaacctg ctcggcatca ccaccagcgt ccactgtgtg gcaggcgtgg
     5521 tggactatgt agtaaaaatc attgaacgcg atctgctacc gaaatacccg aacgtcgatg
     5581 gcgtggtggg gctgaatcat ttgtacggtt gtggcgtggc gattaacgca ccggcggcag
     5641 ttgtacctat tcgtaccatt cacaatattt cgctgaaccc taactttggc ggcgaagtaa
     5701 tggtgattgg cctgggttgt gaaaagttgc agcctgagcg cctgctgact ggaacggatg
     5761 atgtgcaagc tattccagta gaaagcgcca gcattgtcag tttgcaggat gaaaagcatg
     5821 tcggttttca gtccatggtc gaggatattt tgcaagtcgc cgaacgccat ctacaaaaac
     5881 tgaatcaacg tcagcgagaa acctgtccga cttcagaact ggttgtcggc atgcagtgcg
     5941 gtggcagcga tgcgttttct ggcgtaacgg caaacccggc ggttggctat gcgtctgatc
     6001 tactggtgcg ctgcggcgca acggtgatgt tttcagaagt cacggaagtg cgtgacgcga
     6061 tccatctgct gacaccacgc gcagtgaacg aggaggtcgg caaacggctg ctggaggaga
     6121 tggagtggta cgataactat ctcaatatgg gaaaaaccga ccgcagcgcc aacccttcgc
     6181 cgggcaacaa gaaaggcggt ctggcaaacg tggtagagaa agcgctcggc tccattgcta
     6241 aatcgggcaa aagtgcaatt gttgaagtgc tgtcgcccgg tcaacgcccg actaaacgcg
     6301 gattaattta cgccgcgacg ccagccagcg attttgtctg tggcacgcaa caggtggctt
     6361 cgggtatcac cgtgcaagtg tttacgaccg gtcgtggtac gccgtacggc ctgatggcgg
     6421 tacccgtcat taaaatggca acccgcaccg agctggcgaa ccgctggttt gatttaatgg
     6481 atattaatgc gggcaccatc gctaccggcg aagaaactat tgaagaggtg ggctggaagt
     6541 tgttccactt tattctcgac gtcgccagcg ggaagaagaa aaccttctcg gatcaatggg
     6601 gactgcataa ccagctggcg gtgtttaacc cagcaccggt gacctgataa ttcaattcgt
     6661 attatgcccg atagcacacg tttatcgggc atattgagtc acagcgaaga cgcgtgatcc
     6721 ccgaccagaa tcacttcaac cccagccttt cgcagtcctt ccagactatc cgcaggaatg
     6781 ccttcatcaa caatgatcat gtcgatacgt tgagtatcaa tgatcttatg taaactcgaa
     6841 cgattgaatt tactggaatc ggtgaccacg atgatccgtt ccgcaacttc gcacatccga
     6901 cggtttaaac gggcttcatc ttcattatgt gtgctgacgc cgcgctccag atcgatcgca
     6961 tctacaccaa gaaacagcat atcgaagtgg taattttgca gcgattgctc agcctgatcg
     7021 ccgtaaaaag attgcgactg acggcgcaaa tgcccgccgg tcatcagcag ctcaacgcct
     7081 tccgcttcca gcaacgcatt agccacgttc ataccgttgg tcatcgcaat tacgtcagtg
     7141 tacttgcgca tcagacgagc aatctcaaaa gtggtggtcc cggaatcgag gatcacccga
     7201 tgacctggct gaatcaactc aacggcagct ttcgcaacgc tgcgtttcat cgcggtgttc
     7261 agtgcgcttt tatcttccac tgatggctcg actgacggcg tcgtgctatc gcagatcaac
     7321 gcgccaccat aggcacgcac agcgatcccc tgcttttcca gaaaagccag atcattgcgg
     7381 atcgtcacag tagatacgcc atacaatgcc gacagatcgt taacctgcac actcccttgc
     7441 tgtcgcagac gctgaatgat ctgttctcgt cgctcgctgg tgcctgtcac tcgcttctca
     7501 cctgaagcgt cggtattact catagtaagt cctttcgtaa aactttcgtt tcatttcgtt
     7561 ttgcctatta acgcctttct attaagcaaa tgcaagccca tcttgcccat tggcgcaagc
     7621 tactctcgtt tcagtgactt tcattatgtt tcttttgtga atcagatcag aaaactatta
     7681 tatttcgttt tatttttatc tcaccatgat gcagtatcaa ttgaaacaaa acgaaagatt
     7741 aatatcgcag taatctgaac tggagaggaa agtgaaacat ctgacagaaa tggtgagaca
     7801 gcacaaagcg ggcaaaacaa atgcaattta tgccgtttgt tccgcacatc cgctggtgct
     7861 ggaggctgca atccgctacg ccagtgcaaa ccaaacgccg ttactgattg aagcaacctc
     7921 caatcaggta gaccagttcg gcggttatac cggaatgacg cccgccgatt ttcgcggctt
     7981 tgtttgtcag ctcgccgact cgttgaattt cccgcaggat gcgctgattc tgggtggtga
     8041 ccatctgggg ccaaaccgct ggcaaaacct gccagccgct caggcaatgg ccaatgccga
     8101 tgatttgatt aaaagctacg ttgcggcagg attcaaaaaa atccaccttg attgcagcat
     8161 gtcctgtcag gacgatccaa ttcccttaac tgatgacatc gtggctgaac gcgccgcccg
     8221 tctggcgaaa gtggcggaag aaacctgtcg cgaacacttt ggcgaagccg atctggagta
     8281 tgtcattggt accgaagtgc cggtacctgg cggcgcgcat gaaaccttaa gcgagctggc
     8341 ggtcaccacg ccggatgccg cccgcgccac gctggaagcc catcgtcacg cctttgaaaa
     8401 gcaaggtttg aatgccatct ggccacgcat cattgccctg gtggttcaac ccggcgtcga
     8461 attcgatcac accaacgtta ttgattatca gcccgccaaa gcgagcgcct taagccagat
     8521 ggtcgaaaac tacgaaacgc tgattttcga agcgcactct accgattatc aattgtttta
     8581 agccggattg atgaacgctt gattcacggt caggtcggcg ttcagtgggt cggatttgcg
     8641 ggggcaaatc tggtgctggt agccaacgat gaggttgccg aagatccggt acaacaaaac
     8701 ctgatggaaa tggtactggc agaagggatc gccgtacgtt tctggacgct gcaaaaagtt
     8761 atcgacaaca ttcatcgcgc cgccgatcga cagaaaatcc tgctggtttg taaaacaccc
     8821 gccgatttcc tgacgctggt gaaaggtggc gttccggtga atcgcattaa cgttggcaat
     8881 atgcactacg ccaatggcaa acaacaaatc gccaaaacgg tttctgtgga tgcgggcgat
     8941 atcgcagcat ttaacgacct gaaagccgct ggggtggaat gcttcgttca gggcgtcccg
     9001 acagagcctg ctgtggacct ctttaaatta ctttgaggga ttcatcatgg aaatcagcct
     9061 gttgcaggca tttgcgttgg gcattatcgc ctttatcgct ggcctagata tgtttaacgg
     9121 cttaacccat atgcaccgcc cggtggtcct cggcccgttg gtcgggctgg tacttggcga
     9181 tctgcatacc ggaattttaa ccggcggtac gctggaacta gtgtggatgg ggctggcccc
     9241 gctggcgggc gcacagccgc ctaacgtgat tatcggtact atcgtcggca cggcgtttgc
     9301 cattactact ggcgtgaaac ccgatgtcgc ggtcggtgtc gccgtacctt tcgctgtcgc
     9361 agtacagatg gggattacct tcctgttctc ggtgatgtct ggcgtgatgt ctcgctgcga
     9421 ccggatggcg gagaacgccg atactcgcgg cattgaacgc gtgaactatc tggcactgct
     9481 ggcactcggc actttctatt ttctctgcgc tttcctgcct atctacttcg gcacggaaca
     9541 tgcaaaaacc atcattgatg tcctgccgca acgattaatc gacggcctcg gcgtcgcggg
     9601 cggcatcatg ccagcaatcg gttttgccgt gctgctgaaa atcatgatga aaaacgtcta
     9661 cataccctac ttcatcctgg gctttgttgc cgcagcctgg ctcaagttac cggtgctggc
     9721 tattgctgcc gccgcgctgg cgatggcgct gatcgatctg ctgcgtaaat ctcctgaacc
     9781 gactcaacct gcggcacaga aagaggaatt tgaagatggc atctaatcaa accaccctgc
     9841 cgaacgtcag cgaaaatgaa gaaacactgc tgactggcgt caatgaaaac gtgtatgaag
     9901 atcggagcat tgccgcggag ctgacgaaaa aagatatcaa ccgcgtcgcc tggcgttcca
     9961 tgctgttaca agcgtctttt aactacgaac gtatgcaggc ttctggctgg ttgtacggtc
    10021 tgctgcctgc tctgaaaaag atccacacta ataaacgcga cctggcgcgc gccatgaagg
    10081 ggcatatggg tttcttcaat acccatccgt ttctggtgac gtttgttatc ggcattatcc
    10141 ttgcgatgga gcgttctaag caggacgtta acagtattca gagcaccaaa attgccgtcg
    10201 gtgcgccgct tggcgggatt ggcgatgcga tgttctggct aacgctactg ccgatttgtg
    10261 gcgggatagg cgcaagcctc gcgctacaag gctctattct tggcgcagtc gtctttattg
    10321 tgctgttcaa cgtggtacac ttggggctgc gttttggtct ggcgcattat gcttaccgca
    10381 tgggcgtggc ggcgattccc cttattaaag cgaataccaa aaaagtcggc catgctgcgt
    10441 caatcgttgg aatgacggta atcggcgcac tggtggcaac ctatgttcgt ttaagcacca
    10501 cgctggaaat cacggcgggc gacgcagtgg ttaagttaca ggctgatgtt atcgacaaac
    10561 tgatgcctgc cttcttaccg ctggtctaca ccctgaccat gttctggctg gtacgccgcg
    10621 gctagagtcc gctgcgcctg attgcggtta ccgtggttct agggatcgtc ggtaaattct
    10681 gccatttcct ttaaatacaa tgaggtatta gatgttaagt attattttga cagggcatgg
    10741 cgggtttgcc agtggcatgg aaaaagcaat gaagcaaatc ctcggcgatc aatcgcagtt
    10801 tattgccatc gattttcccg aaacctcaag cgccgcgctg cttacatcgc agcttgaaga
    10861 ggccatcgcc caactggatt gtgaagacgg cattgttttt ctgacggatt tgctgggcgg
    10921 cacgccgttt cgcgtagctt caacgctggc gatgcaaaaa ccgggctgtg aagtgattac
    10981 cggcaccaat ttgcaactgc tactggagat ggtgctggag cgcgaagggt taagcggtga
    11041 agaattccgt gtacaggcgc tggagtgtgg acaccgaggg ctgaccagtc tggtggacga
    11101 gttaggccgc tgccatgaag aatatccggt cgaggaagga atatgacaca cgttctgcgc
    11161 gccagaaggc tgctgactga agagggatgg ttcgatgacc atcagttgcg tgttgctgac
    11221 ggtgtcatcg cagcaatcga accgattcca gtgggcgtga ctgaacgcga tgcggaactg
    11281 ctctgccccg cttacatcga cacccatgta cacggtggtg cgggcgttga tgtaatggat
    11341 gacgcgccgg atgtactcga caagctggca atgcacaagg cacgcgaagg tgtcggcagt
    11401 tggttaccga ccaccgtaac cgcgccgctt aataccattc atgcggcgct gaaacgtatt
    11461 gctcaacgtt gccaacgcgg cggacctggt gcgcaagtgc tggggagtta tctcgaagga
    11521 ccgtacttca cgccgcagaa taaaggcgcg catcc
//
LOCUS       DADMEQ010000120        11437 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.121, whole genome shotgun sequence.
ACCESSION   DADMEQ010000120 DADMEQ010000000
VERSION     DADMEQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.121"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            9..461
                     /locus_tag="JNZ05_003286"
     CDS             9..461
                     /locus_tag="JNZ05_003286"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8566405.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            707..1751
                     /locus_tag="JNZ05_003287"
                     /pseudo
     CDS             707..1751
                     /locus_tag="JNZ05_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1867..2040
                     /locus_tag="JNZ05_003288"
     CDS             1867..2040
                     /locus_tag="JNZ05_003288"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566406.1"
                     /translation="MKKALDRLIKEAARQDVAVTVLETETGRYSDTLRSALISLDGDN
                     AWALSESWCGTIQ"
     gene            2095..2427
                     /locus_tag="JNZ05_003289"
                     /pseudo
     CDS             2095..2427
                     /locus_tag="JNZ05_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
     gene            complement(2484..3941)
                     /gene="pepD"
                     /locus_tag="JNZ05_003290"
     CDS             complement(2484..3941)
                     /gene="pepD"
                     /locus_tag="JNZ05_003290"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY8566407.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            4203..4661
                     /gene="gpt"
                     /locus_tag="JNZ05_003291"
     CDS             4203..4661
                     /gene="gpt"
                     /locus_tag="JNZ05_003291"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY8566408.1"
                     /translation="MSEKYIVTWDRLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4753..5997
                     /gene="frsA"
                     /locus_tag="JNZ05_003292"
     CDS             4753..5997
                     /gene="frsA"
                     /locus_tag="JNZ05_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY8566409.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            6055..6456
                     /locus_tag="JNZ05_003293"
     CDS             6055..6456
                     /locus_tag="JNZ05_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY8566410.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(6495..7550)
                     /gene="phoE"
                     /locus_tag="JNZ05_003294"
     CDS             complement(6495..7550)
                     /gene="phoE"
                     /locus_tag="JNZ05_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY8566411.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            7838..8941
                     /gene="proB"
                     /locus_tag="JNZ05_003295"
     CDS             7838..8941
                     /gene="proB"
                     /locus_tag="JNZ05_003295"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY8566412.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8953..10206
                     /gene="proA"
                     /locus_tag="JNZ05_003296"
     CDS             8953..10206
                     /gene="proA"
                     /locus_tag="JNZ05_003296"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY8566413.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            10321..10396
                     /locus_tag="JNZ05_003297"
     tRNA            10321..10396
                     /locus_tag="JNZ05_003297"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10354..10356,aa:Thr,seq:cgt)
     gene            complement(10411..>11437)
                     /locus_tag="JNZ05_003298"
     CDS             complement(10411..>11437)
                     /locus_tag="JNZ05_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY8566414.1"
                     /translation="WRVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWMLH
                     FREAILSDITTEKIMEAVDGMENRRHRLNWEMSRDRCLRLGNPVPEYKPKLASKGTKT
                     RHLAILRAILNMAVEWGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPVV
                     MFAITTGLRRSNVTDLEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQG
                     LHKRWVFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWAS
                     WLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEEN
                     VVYLNAR"
BASE COUNT         2747 a         2836 c         2946 g         2908 t
ORIGIN      
        1 tttatattat gaataacata caaataagag attatcagcc tggcgatttt cagcaactat
       61 gcgctatttt cattagagcg gttacgatga ccgtcagtca gcattattca ccacaacaaa
      121 ttgccgcctg ggcgcagatt gacgaatctc gctggaagga gaaactcgcg aaatcacaag
      181 tgcgggttgc agtcattaat gcaaaactgg tcggctttat tacctgcgtt gaacactata
      241 tcgatatgtt atttgttgac cctgaataca cccgccgtgg ggttgccagc gctttgttaa
      301 aaccttttat taagtctgaa tccgaactta cggtggacgc aagcataacc gcaaaaccct
      361 tttttgaacg ttatggtttt cagacagtta agcagcagcg cgttgaatgc cggggagcgt
      421 ggtttattaa tttttatatg cgttataaac cgcaacatta aatccagctt gcaatgaaaa
      481 taacgcccgc ctagtatgtg cgcgttttct tattccacaa actgcaagga ggtaaatcat
      541 gacaaatcct ttatcaatga ctctttgcag acctttccag gattaattct ttttttcttg
      601 ccctggattc gtctgccatt tcctgatttt ttaattgtta ctctcaataa ttcaagtcgc
      661 agcacatgca acttgaagta tgacgagtct atttacggaa tggtatatgg gcaattatat
      721 tcgtccctta tccgatgcgg tatttaccat cgcatctgat gacctgtgga tcgagagttt
      781 agcgatccaa caattacaca ccacggcaaa tttacccaac atgcagcgcg tagtggggat
      841 gccagattta caccccggac gcggctaccc gattggcgca gcgttcttct ccgcaggccg
      901 tttttaccca gcactggtcg gcaatgatat cggctgtggc atggcgctat ggcaaacaga
      961 tattctcgct cgcaaataca acgccgataa gtttgaaaag cgattatcag ctttggatga
     1021 cgttgctgaa gaaagctggc tggaggaaaa cctgccgtca gcatttgcac agcatccgtg
     1081 gcgcagctcg cttggctcta tcggtggtgg caatcatttc gctgaactgc aacaagttga
     1141 tcaaatcatc aacgctgaac tgtttgcact ggcaggtctg gatgcgcagc atctgcaact
     1201 gctggtccat agcggctcga gggagctagg gcagtctatt ttacagcggc atattgcctc
     1261 gttttcgcat catggtttgc ctgaaggcag tgacgacgcg ctggcgtttg cgcgttttaa
     1321 tcgccacctg atcgctttgc gcataatgca acaggttaag gccaccggaa gtccggttct
     1381 ggatgtggcg cataactttg ttagcgcgtg tcgaatcggt gatcaacaag gggtcttaca
     1441 tcgtaaaggt gccacgcctg atgactgtgg tctggtggtt attcccggtt cacgcggtga
     1501 ttactccagg ctggttcagg cccgtcagga gtgaggaaac attgcattcg ctggcgcatg
     1561 gggctgggcg taaatggggg cgcaccgagt gtaaagggcg tctggcagcg aaatacacag
     1621 cgacgcaact ctcgcgtact gaacttggca gtcgggtaat ttgtcgcgat aaacaactca
     1681 tcttcgaaga agcgccacaa gcctataaat cggctgaaag tgtggtgcaa tgtctgggca
     1741 ggccggatta attattcctg tcgcgcggct gcgtccggtg ctaacgctca aaaacagtgg
     1801 agggaaaaaa ggatgatctt gctataactc tcctcagcgc aggggccgga agaatgttgt
     1861 ctcgcagtga aaaaggcgct ggacaggcta attaaagaag ctgcccgaca agacgtcgcg
     1921 gtaacggtgc tggaaacaga aacaggccgc tactctgaca cgctgcgttc ggcgctgatt
     1981 tctctggatg gcgacaacgc atgggcgtta agcgaaagct ggtgtggcac tattcagtag
     2041 atttgcccga gcccgtatcg cccacatcac gggcgtaaaa actggtttct gggcattggg
     2101 cgttttaccg ctgatgagca ggagcaatcc gatgcgatcc ggtatgagac gctgcgttcg
     2161 tcggggccgg gcggtcaaca tgtcaataaa accgactcgg cggtacgcgc cacgcatttg
     2221 gcatccggta ttagcgtgaa ggttcagtca gagcgcagcc agcatgctaa caaacggctg
     2281 gcgcgattgc tgattgccag gaagctggat cagcagcaat aggaaaatag cgcgtagctg
     2341 aaatcacagc ggcgaatgtt ccatcaccag attgaacgtg gcaacccgcg acgaacgttt
     2401 acagggatgg cttttatcga aggataatga aggatgaaac tgccggaagg cgattaaacg
     2461 ccatccggca gcaaatcaaa taattacttc gccggaattt ctttcagcag ttcagtcagc
     2521 agtgtccagt aatgacctac gctttcgatg tgaacttgct catccggaga gtgtggaccg
     2581 gtgatagttg gcccgataga aaccatgtcc atttccggat acggtttttt gaacagacca
     2641 cattccaggc ccgcgtggat aatctggatg ttcggcgtct tgttgaacag gcgctgatag
     2701 gtttcacgta ccagatgcat caccggagaa ttagcgtccg gctgccagcc aggatatgcg
     2761 cctttcgctt cggttttcgc gccagccagt ttacccagcg aatccagcat gctcaccacg
     2821 tagtctttac cgctgtcgat cagtgaacgg atcaggcagt gaatttctac gttattgtca
     2881 gtcatggtca ccacaccgac gttcagggag gtttcgacca caccttttgc cacgtcggaa
     2941 ttgcggatca caccgttcgg ggtggcgttc agcagacgaa taaaggtatc gcgagatttc
     3001 gcaatcaggg cagctttatc gttcgctaca gagtccagca acaaggccag atttttctct
     3061 ttctctgcca gctcgttttt caggatctcc tgataggtat tcaccagaga tttcagtgcg
     3121 tcgactttat cagctgtgac agcaatggtc gcaaaggctt cacgcgggat ggcgttacgc
     3181 agtgtgccgc cgttgaaatc gataaggcgc aggtccagtt cttctgcatg acccgccagg
     3241 aagcgcacca gcagtttgtt ggcattaccc aggccaacgt ggatttcacc gccggagtga
     3301 ccgcctttca gaccttttaa ggttaacttg aaggtttcaa aaccagctgg aaccgcttca
     3361 cgatctaaat gcaggttgga ggtgaagtcg ataccccccg cacaacccat gtagatttca
     3421 ccttcttctt cggagtcggt gttaatcaga atatcagcct gcaaccagtt gctctgtaag
     3481 ccgaacgcac cgtccatacc ggcttcttcg gtcatggtca gcagcacttc cagcgggccg
     3541 tgaaccacgt tttcgtcagc cagaaccgcc agtgcagagg ccataccaat gccgttatcc
     3601 gcacccagcg tggtgccgcg cgctttaacc cattcgccat caatataagg ctggatagga
     3661 tctttcgtga agtcatgcac ggtgtcgtta tttttctgcg gcaccatatc gaggtgggcc
     3721 tgtaagacga ccggtttacg attttccata cctgcggtag caggtttacg aatcaggata
     3781 ttacctacct gatcgcgttc gacatggaaa cctttctctt ttgcccaacc aacaatgtat
     3841 tcagcgagtt gctcttcatg ataggacggg tgaggaatag aacagatttt ggcaaaaata
     3901 tcccacagcg gctgtggaga taattgagac agttcagaca cgttaagtct ccttgtcgat
     3961 cacccgcaaa acagtattgc aggtcacagg gttagcagaa aatgttgtca acacaagaca
     4021 ggcttgcgag atatgtattg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
     4081 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatatc tataatctcg
     4141 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
     4201 acatgagcga aaaatacatc gtcacctggg acaggttgca gatccatgca cgtaaactcg
     4261 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
     4321 tggtaccggg ggcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta
     4381 tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg
     4441 atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact gctgttgcga
     4501 ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc
     4561 cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggatcgaa cagccgtggg
     4621 atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcat
     4681 tgccgggcgt tgttcttttt aacttcaggc aggttacaat agtttccagt aagtattctg
     4741 gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa
     4801 catcctgaaa cctcgacgct agtccgccgc tttaatcacg gtgcacaacc gcctgtgcag
     4861 tcggctcttg atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg
     4921 atctggcgcg gtattgaccc acgtgaaatc ctcgacgtcc aggcacgtat tgtgatgagc
     4981 gatgctgaac gtaccgacga tgatttatac gataccgtga ttggctaccg tggtggcaac
     5041 tggatttatg agtgggccac ccaggcgatg gtgtggcaac aaaaagcctg tacggaagaa
     5101 gatccgcaac tcagtggtcg acactggctg catgcggcta cgttgtacaa cattgccgcc
     5161 tatcctcatc tgaaaggaga tgacctggcc gagcaagcgc aggctttatc aaaccgcgcc
     5221 tatgaagagg ccgcccagcg tctgccgggc acgatgcggc agatggagtt taccgtaccc
     5281 ggcggtgcgc ccatcaccgg ctttttgcat atgccgaaag gcgatggccc gttcccgaca
     5341 gtattaatgt gtggtggtct ggatgcgatg cagacggact attacagcct gtatgaacgt
     5401 tattttgcgc cgcgcggcat tgcgatgctg actattgata tgccgtcggt gggcttttct
     5461 tcaaaatgga agctcaccca ggactccagc ctgttgcatc agcacgtctt aaaggcgctg
     5521 cctaacgtac cgtgggtgga tcacactcgc gtcgcggcct ttggtttccg tttcggcgct
     5581 aacgttgccg tgcgtttggc ataccttgaa tcgccgcgtc tgaaagcggt tgcctgtctt
     5641 ggtccggtag ttcataccct gttgagtgat tttaagtgcc agcaacaggt gccggaaatg
     5701 tatcttgacg ttctggcgag tcgtttgggg atgcatgatg cttccgacga agcgttacgc
     5761 gtggagctga atcgctattc attaaaagtg caaggattgc tggggcgtcg ctgcccaacg
     5821 ccaatgttat caggctactg gaagaacgat ccgttcagcc cggaagagga ctcacgctta
     5881 atcacctcat catctgctga cggtaaatta ttagagatcc catttaaccc ggtgtatcgg
     5941 aattttgaca aaggtcttca ggaaatcacc ggctggatcg aaaaacgctt gtgttaaaaa
     6001 tttgctaaat tttgccaatt tggtaaaaca gttgcatcac aacaggagat agcaatgacg
     6061 ttaccgagtg gacacccgaa gagcagattg atcaaaaaat ttaccgcact aggcccgtat
     6121 attcgtgaag gtaagtgcga agataatcga ttctttttcg attgtctggc tgtatgcgtc
     6181 aacgtgaaac cggcaccgga agtgcgtgaa ttctggggct ggtggatgga gctggaagcg
     6241 caggaatccc gttttaccta cagctaccag tttggtctgt tcgataaagc aggcgactgg
     6301 aagagtgttc cggtaaaaga tactgaagtg gttgaacgac tggagcacac cctgcgtgag
     6361 tttcacgaga agctgcgtga actgctgacg acgctgaatc tgaagctgga accggcggat
     6421 gattttcgtg atgaaccggt gaagttaacg gcgtgagtga aatgtgccgg atgcatcaca
     6481 tccggcaata ttcattaaaa ctgatacgtc atcccaaccg cgacagtatc atcattatta
     6541 atattcagct tattatcgct atccagtttg ttgattttat aatcaacaaa cgctgacata
     6601 tttttgttga aataatacgt agcaccgacg tcgatataat tgaccagatc ttcatcaccg
     6661 atgccctcaa tatctttccc tttcgataag acataaccca gcgatggacg cagaccaaag
     6721 tcaaactggt attgagcgac cgcttcaaag ttctgtgtct tattggcaaa gccgccagtt
     6781 attggcgtca ttttgcgtgt ttcagaatag aaagttgcca gataaatatt attggcatcg
     6841 tatttcagac ctgttgccca tgcttctgca cgcttgcctg tgccacggct ttgcaggttc
     6901 tgctcgttgg tgcgatctga gttggtatag gccccactaa tggcgaaatc gctgccgcca
     6961 aagtcatatg tcaatgacgt gccgaagcca tcgccgtttt gctttttaac gtcgcggttt
     7021 tcgtttttcc cttgatattg cagggttaag ttcaggccat cgataacgcc gaagaagtcg
     7081 gtgttccgat acgtcgccag accgctggcg cgtttggtca taaagttgtc ggtctgcgcc
     7141 gaggaatcgc caccaaattc cgggaacata tcggtccagg cttccacgtc atacaacgcg
     7201 cccaggttac gaccataatc gaaagaaccc aaatctttat atttcaaccc ggcaaaagcg
     7261 agacgcgttt tttgctgtgc agtatcactc tccgctttat ttccggcaaa ctccgcttcc
     7321 caccgaccat aaccagtcag ttgatcgtta atttgagttt cacctttaaa accaaaacgg
     7381 atataactct ggtcgccatc tttactggcg ttatcactca tataatgcat ggctttaact
     7441 ttgccataga catccagttt attaccgtct ttattatata tttctgcagc ctgtacagat
     7501 gcagatgcca caatgcccat taccactaat gccagagtgc tctttttcat tttcattcct
     7561 gattttaatt aacgcgcgaa tattcagcgg gagagccccg ttgaaaacag gaaagttttt
     7621 aacctgagat tgttaaagat atattacaga ttaatgatat tctgaaaatg tggtaattta
     7681 ttaaatctgt aataaaagcg taaacaactg ccgctacgct tgctgatccc gcgcaacaaa
     7741 acgccatgct ttgctcgcag atggttggca accgacgaca gtcctgctaa aacgttcgtt
     7801 tgatatcatt tttcctaaaa ttgaatggca gagaatcatg agtgacagcc agacgctggt
     7861 ggtaaaactc ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc gtgcccatat
     7921 cgttgaactt gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga ttgttattgt
     7981 gacgtcgggc gcgatcgccg ccggacgtga gcacctgggt tacccggaat tgccagcgac
     8041 tatcgcctcg aaacaactgc tggcggcggt agggcagagt cgactgattc aactgtggga
     8101 acagctgttt tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc gtgctgatat
     8161 ggaagaccgt gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc tcgatcacaa
     8221 tatcgttccg gtaatcaatg agaacgatgc tgtcgctacg gcagagatta aggtcggcga
     8281 taacgataac ctttctgcgc tggcggcgat tctggcgggt gccgataaac tgttgctgct
     8341 gaccgatcaa aaaggtttgt ataccgctga tccgcgcagc aatccgcagg cagaactgat
     8401 taaagatgtt tacggcattg atgacgcact gcgcgcgatt gccggtgaca gcgtttcagg
     8461 cctcggaact ggcggcatga gtactaaatt gcaggccgct gacgtggctt gccgtgcggg
     8521 tatcgacacc attattgccg cgggcagcaa gccgggcgtt attggtgatg tgatggaagg
     8581 catttccgtc ggtacgctgt tccatgccca ggcgactccg cttgaaaacc gtaaacgctg
     8641 gattttcggt gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa ctgccgccat
     8701 tctggaacgc ggcagctccc tgttgccgaa aggcattaaa agcgtgactg gcaatttctc
     8761 gcgtggtgaa gtcatccgca tttgcaacct cgaaggtcgc gatatcgccc acggtgtcag
     8821 tcgttacaac agcgatgcat tacgccgtat tgccggacac cactcgcaag aaattgatgc
     8881 aatactggga tatgaatacg gcccggttgc cgttcaccgt gatgacatga ttacccgtta
     8941 aggagctggc cgatgctgga acaaatgggc attgccgcga agcaagcctc gtataaatta
     9001 gcgcaactct ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga tgaactggaa
     9061 gcacaaagcg aaatcatcct caacgctaac gcccaggatg ttgctgacgc gcgagccaat
     9121 ggccttagcg aagcgatgct tgaccgtctg gcactgacgc ccgcacggct gaaaggcatt
     9181 gccgacgatg tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt aatcgatggc
     9241 ggcgtactgg acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg ggttattggc
     9301 gtgatttatg aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg cctgaaaacc
     9361 ggtaatgcgg tgatcctgcg tggtggtaaa gaaacctgtc gcactaacgc ggcaacggtg
     9421 gcggtgattc aggacgccct gaaatcctgt ggcttaccgg cgggtgccgt gcaggcgatt
     9481 gataatcctg accgtgcgct ggtcagtgaa atgctgcgta tggataaata catcgacatg
     9541 ctgatcccgc gtggtggcgc tggtttgcat aaactgtgcc gtgaacagtc gacaatcccg
     9601 gtgatcacag gtggtatagg cgtatgccat atttacgttg atgaaagcgc agagatcgct
     9661 gaagcattaa aagtgatcgt caacgcgaaa actcagcgtc cgagcacatg taatacggtt
     9721 gaaacgttgc tggtgaataa aaacatcgcc gatagcttcc tgcccgcatt aagcaaacaa
     9781 atggcggaaa gcggcgtgac attacacgca gatgcagctg cgctgacaca gttgcaggca
     9841 ggccctgcga aggtggtggc ggttaaagcc gaagagtatg acgatgagtt tctgtcatta
     9901 gatttgaacg tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat tcgtgaacac
     9961 ggcacacaac actccgatgc gatcctgacc cgcgatatgc gcaacgccca gcgttttgtt
    10021 aacgaagtgg attcgtccgc tgtttacgtt aacgcctcta cgcgttttac cgacggcggc
    10081 cagtttggtc tgggtgcgga agtggcggta agcacacaaa aactccacgc gcgtggccca
    10141 atggggctgg aagcactgac cacttacaag tggatcggca ttggtgatta caccattcgt
    10201 gcgtaaataa aaccgggtga tgcaaaagta gccatttgat tcacaaggcc attgacgcat
    10261 cgcccggtta gttttaacct tgtccaccgt gattcacgtt cgtgaacatg tcctttcagg
    10321 gccgatatag ctcagttggt agagcagcgc attcgtaatg cgaaggtcgt aggttcgact
    10381 cctattatcg gcaccattta aatcaataag ttacctcgca tttaagtaaa ccacgttctc
    10441 ctcttgtgcc gtatttgtgc cattgcgact tataatcgca tcgattttgc ttgcgtgctc
    10501 ggtgagatgc ccggctgaaa ggtgggcgta tctttgaacc atttcgagag tttcccatcc
    10561 tcccatctct ttaagtgcaa gaagagagac accggactga accagccagc ttgcccaggt
    10621 atgcctcaga tcatggaagc ggaagttgct aatgcctgcc cgctttaacg ctcccttcca
    10681 tgccttgttg ctgtcggttc tcatcttcct taccgctgct gtttttgttc cgtcgcttcg
    10741 gtaggcaggt ttggtgtgga caaataccca tctcttatgg agcccctgct gttttcttaa
    10801 tatctggcat gcggtttcgt taagaggaac tccgatcgca ttgccagctt ttgtttcatc
    10861 agggtgcatc catgccattt tcttatccag atcgacctgt gaccactcaa ggtctgtaac
    10921 gttggaacgg cgaaggcctg tcgtgattgc aaacatgacc acagggaaga agtgaggagc
    10981 aatttctgca aacaggcgct tcgattcctc ctctgtaagc catctgatgc gtccattctt
    11041 aacgcgtggt gttgatattt tgggtgccct gtcaagccat ccccattcaa cagccatatt
    11101 gagaatggcg cgaagtattg ccagatgcct cgtcttcgtt cctttgcttg caagctttgg
    11161 tttatactcc ggcaccggat tgccaagtcg caaacacctg tcccggctca tctcccagtt
    11221 caggcgatgg cggcggtttt ccatcccgtc taccgcctcc attatttttt ctgttgttat
    11281 gtcagagaga atggcttctc tgaagtgcaa catccagaac gatataatgc tcttgtcatc
    11341 atcaatggac ttcttatccg atttctcacg cagccaccgt atgcaggctt ccttgaatag
    11401 ctttttcggt gattccccga gattttttac tctccac
//
LOCUS       DADMEQ010000121        11435 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.122, whole genome shotgun sequence.
ACCESSION   DADMEQ010000121 DADMEQ010000000
VERSION     DADMEQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.122"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="JNZ05_003299"
     CDS             complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="JNZ05_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAY8566415.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            321..1952
                     /gene="dcuS"
                     /locus_tag="JNZ05_003300"
     CDS             321..1952
                     /gene="dcuS"
                     /locus_tag="JNZ05_003300"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HAY8566416.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1949..2668
                     /gene="dcuR"
                     /locus_tag="JNZ05_003301"
     CDS             1949..2668
                     /gene="dcuR"
                     /locus_tag="JNZ05_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HAY8566417.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYDFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3239..4579
                     /locus_tag="JNZ05_003302"
     CDS             3239..4579
                     /locus_tag="JNZ05_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY8566418.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQL
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4657..6303
                     /locus_tag="JNZ05_003303"
     CDS             4657..6303
                     /locus_tag="JNZ05_003303"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HAY8566419.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6426..7055
                     /locus_tag="JNZ05_003304"
     CDS             6426..7055
                     /locus_tag="JNZ05_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HAY8566420.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7194..8603)
                     /gene="melB"
                     /locus_tag="JNZ05_003305"
     CDS             complement(7194..8603)
                     /gene="melB"
                     /locus_tag="JNZ05_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HAY8566421.1"
                     /translation="MTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLF
                     LVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEGTTQI
                     VFVCVTCILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAGVTL
                     PFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHLTLKA
                     IVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANL
                     VTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGT
                     ALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGY
                     VPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKV
                     PPEPVHADIPVGAVSDVKA"
     gene            complement(8718..10073)
                     /gene="melA"
                     /locus_tag="JNZ05_003306"
     CDS             complement(8718..10073)
                     /gene="melA"
                     /locus_tag="JNZ05_003306"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAY8566422.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            10356..11264
                     /gene="melR"
                     /locus_tag="JNZ05_003307"
     CDS             10356..11264
                     /gene="melR"
                     /locus_tag="JNZ05_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HAY8566423.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT         3004 a         2937 c         2762 g         2732 t
ORIGIN      
        1 gactttcgtt tgagattaaa taacttgccg ttgccacgta cgcaattgcc agaatgctga
       61 catatcgcag tgttgaaata gacatcgatc ttttcgccgg tataacagcg ataacccccg
      121 tccagtagcg cctgatccat gaccttccct ctctcatcag tatgattatc gctataagca
      181 tagccccgga atcttcatac agcactggca gtttccgttg ccagagtctc ttcccctata
      241 tagccaatca tttttactcc ctttgaatta cccgcctcat cagagataat gcttaagaaa
      301 tttgtcacac aaggaagctg atgagacatt cattgcccta ccacatatta cgcaaacgtc
      361 cgatgaaatt gagtaccact gtgatcttaa tggtcagcgc ggtactgttc tcggtgctat
      421 tggtggtgca tctgatttac ttctcgcaaa tcagtgatat gacgcgagac gggctggcca
      481 acaaggcact ggcagtggcg cgtaccctcg ccgactcgcc ggaaatccgt cagggcttgc
      541 agaaaaaacc gcaggagagt ggcatccagg ccatcgcgga agccgtgcgc aaacgcaacg
      601 atctgctgtt tattgtcgtt accgatatgc aaagtcttcg ctactcacat cctgaagccc
      661 agcgtattgg tcagccattt aaaggtgatg acatccttaa tgcgctgaat ggcgaagaaa
      721 atgtcgctat caatcgcggt tttctggcgc aggctttacg cgtatttacc cccatctacg
      781 atgaaaatca taaacaaatt ggcgtggtgg cgatcggcct tgagttaagc cgcgtgaccc
      841 aacagatcaa tgacagtcgc tggagcatta tctggtcggt attatttggc atgctggtcg
      901 gactgattgg cacctgcatt ctggttaagg tactgaaaaa aatccttttc ggcctggaac
      961 cctacgaaat ctccacgctg tttgagcaac gccaggccat gttgcagtct atcaaagaag
     1021 gcgtcgttgc cgtggacgat cgcggcgagg tcacgctgat caacgatgcc gcacaagaat
     1081 tgctgaatta ccgtaagtcg caggacgatg agaaactgtc gacgctcagc cactcatggt
     1141 cacaggtggt agatgtctcg gaagtgttac gcgacggtac gccgcgccgc gacgaagaga
     1201 ttacgattaa agaccggcta ttactgatca acaccgttcc ggtgcgcagt aatggcgtta
     1261 tcatcggtgc catttcaacc ttcagggaca aaactgaagt tcgtaaactg atgcagcgac
     1321 tcgacggtct ggtcaactat gctgacgcac ttcgtgaacg atcccacgaa tttatgaata
     1381 aattgcatgt gattctcgga ttattgcatc tgaagagtta taagcagttg gaagattaca
     1441 ttctcaaaac agccaataac tatcaggaag agattggctc tctgctgggt aagatcaaat
     1501 ctccggttat cgctggtttt ttaatcagca agattaaccg cgcgaccgat ttaggccata
     1561 cgctgatttt aaacagtgaa agccagctgc cagacagcgg cagtgaggac caggtcgcga
     1621 cgctgattac cacattggga aatctgatag aaaacgcgct ggaagcatta gggccggaac
     1681 ccggtggcga aattagcgta acattgcact accgtcacgg ctggctgcac tgcgaagtta
     1741 acgatgatgg accggggatc gcacccgaca aaatcgatca catttttgac aaaggtgtct
     1801 cgacaaaagg aagcgagcga ggcgtcggtt tagcacttgt caaacaacag gtagaaaatc
     1861 tcggcggcag catcgccgtg gaatcggaac cggggatttt cacacaattt tttgtccaga
     1921 taccctggga cggggagagg tcgaacagat gatcaatgta ttaattatcg atgacgacgc
     1981 aatggtcgcg gagctgaatc gccgatacgt agcacaaatc ccaggctttc aatgctgtgg
     2041 aacagcctcg acgctggaga aagccaaaga gattatcttc aatagcgatg cgcctatcga
     2101 cctgatattg ctcgatatct atatgcaaaa agagaacggg ctcgatttac tgcctgtcct
     2161 gcataacgcg cgttgcaaaa gtgatgtgat tgtcatctcc tccgcagccg atgcggcaac
     2221 cattaaagat tcgctgcatt acggtgtcgt ggattacctg atcaaaccct tccaggcttc
     2281 ccgctttgaa gaggcgctca ccggctggcg gcaaaagaaa atggcgctgg aaaaacatca
     2341 gtattatgac caggccgagc ttgatcagct aatccacggc agcagctcca acgaacagga
     2401 tcctcgtcgc ttgccaaaag gcttaacgcc gcagacgtta cgcacgctgt gccagtggat
     2461 tgacgcgcat caggactatg acttttcaac cgacgagctg gctaacgagg ttaacatctc
     2521 gcgtgtttcc tgccgaaaat acctcatctg gctggtcaac tgccacatct tgttcaccag
     2581 tatccattat ggcgtcacgg ggcgtccggt ttaccgttac cgtattcagg cagaacacta
     2641 ctcactgctg aaacaatatt gccaataacg gatcgctggt tatctgtaag taataattat
     2701 taccgtaatg caaaataccc ctggcacaag ttacatcgtt aatcagtaaa acttgtgcca
     2761 gatcaaatat aattatccct ccatcatcgt taaaaattaa tatctcttca gatgaacggt
     2821 gtttttaatt tcaaaacgat aacaaaagtt aattaactat tatgtcaccc gcattatgtg
     2881 tatttttacc cacaaatggg tagatcagat taatctataa acctaatgat atctgccctg
     2941 agaacaaaaa atagaccgat aaatatcaat aagataacag caaacaaaac attaacatct
     3001 gcgcagtaca aactataaac ccatcgccag agagtttttc tctctgaaaa agccgcttat
     3061 cacagtgcat aaatttgccg ctgctttaat cagccaatat tcactgtgag gtatttgcta
     3121 aagccggtaa cgaccaaacg gatatttagt caggctctga aaacagttca tacaaaacag
     3181 aacgtgactg tgatctattc agcaaaaatt taaataggat tatcgcgagg gttcacacat
     3241 gttatttact atccaactta tcataatact aatatgtctg ttttatggtg ccagaaaggg
     3301 tggtatcgcg ctgggtttat taggcggtat cggtctggtc attctggtct tcgtcttcca
     3361 ccttcagcta ggtaaaccac cagttgatgt catgctggtt atcattgcgg tggtggcggc
     3421 atcggcgacc ttgcaagctt cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct
     3481 gctgcgccgc aacccgaaat atgtctcaat tgtcgcgccg tttgtgacct gtacactgac
     3541 cattctttgc ggtacgggtc atgtggttta caccattctg ccgatcatct acgacgtcgc
     3601 cattaagaac aacatccgtc cggaacgtcc gatggcggca agttctatcg gtgcacagat
     3661 ggggattatc gccagtccgg tgtcggttgc ggtcgtgtct ctggttgcga tgctgggtaa
     3721 tgtcaccttt gatggtcgcc atcttgagtt cctcgatctg ctggcaatca ccattccatc
     3781 gacgttaatc ggtatcctgg cgatcggtat cttcagctgg ttccgcggta aagatctgga
     3841 taaagacgaa gagttccaga aattcatctc cgtaccggaa aaccgtgagt atgtttacgg
     3901 tgataccgcg acgctgctgg ataaaaaact gccgaaaagc aactggctgg caatgtggat
     3961 tttcctcggg gcaatcgctg tagtcgccct tcttggtgct gattcggacc tgcgtccatc
     4021 cttcggcggc aaaccgctgt cgatggtact ggttattcag atgtttatgc tgctgaccgg
     4081 ggcgctgatt attatcctga ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt
     4141 ccgttccggt atgatcgcca tcgtggcggt gtacggtatc gcatggatgg cagaaaccat
     4201 gttcggtgcg catatgtctg aaattcaggg cgtactgggt gaaatggtga aagagtatcc
     4261 gtgggcgtat gccattgttc tgctgctggt ttccaagttt gtaaactctc aggctgcggc
     4321 gctggcggcg attgttccgg tcgcgctggc gatcggcgtt gatccggcat acatcgtggc
     4381 ttcagcaccg gcttgctacg gttattacat cctgccgact tatccgagcg atctggcagc
     4441 gattcagttt gaccgttccg gcaccaccca catcggtcgc ttcgtcatca accacagttt
     4501 tattctgccg gggttgattg gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc
     4561 gatgtacggg ttcttataaa tgcactttgc gtgccgcccg tgactacgcg acacgccatt
     4621 ttcgaataac aaatacagag ttacaggctg gaagctatgt caaacaaacc ctttatctac
     4681 caggcacctt tcccgatggg gaaagacaat accgaatact atctactcac ttccgattac
     4741 gttagcgttg ccgacttcga cggcgaaacc atcctgaaag tggaaccaga agccctgacc
     4801 ctgctggcgc agcaagcctt tcacgacgct tctttcatgc tccgtccggc acaccagaaa
     4861 caggttgcgg ctattcttca cgatccagaa gccagcgaaa acgacaagta cgtggcactg
     4921 caattcttaa gaaactccga aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc
     4981 ggcaccgcga tcatcgtcgg taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa
     5041 gaagcgctgt cgaaaggcgt ctataacacc tatatcgaag ataacctgcg ctactcgcaa
     5101 aacgcggcgc tggacatgta caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc
     5161 gacctgtacg cggtagatgg cgatgagtac aaattccttt gcgttgcgaa aggcggcggc
     5221 tctgccaaca aaacgtatct ctaccaggaa accaaagccc tgctgactcc cggcaaactg
     5281 aaaaacttcc tcgtcgagaa aatgcgtacc ctcggtactg cagcctgccc gccgtaccat
     5341 atcgcgtttg tgattggcgg tacgtctgcg gaaaccaacc tgaaaaccgt caaattagca
     5401 agcgctcact attacgatga actgccgacg gaagggaacg aacatggtca ggcgttccgc
     5461 gatgtccagc tggaacagga actgctggaa gaggcccaga aactcggtct tggcgcgcag
     5521 tttggcggta aatacttcgc gcacgacatt cgcgttatcc gtctgccacg tcacggcgca
     5581 tcctgcccgg taggcatggg cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc
     5641 aaccgcgaag gtatctggat cgaaaaactg gaacacaatc taggtcagta cattccacaa
     5701 gaactgcgtc aggccggtga aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa
     5761 gagatcctcg cccagctttc gcaatatccg gtttccactc gtttgtcgct cactggcacc
     5821 attattgtgg gtcgagatat tgcacacgcc aagctgaaag agctgattga cgccggtaaa
     5881 gaacttccgc agtacatcaa agatcacccg atctactacg cgggtccggc gaaaacccct
     5941 gccggttatc catcaggttc acttggccca accaccgcag gtcgtatgga ctcctatgtg
     6001 gatctgctgc aatcccacgg cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag
     6061 caggttaccg acgcgtgtca taaacacggc ggcttctacc tcggtagcat cggcggtccg
     6121 gcggcggtac tggcgcagca gagcatcaag catctggaat gcgtcgctta tccggagctg
     6181 ggtatggaag ctatctggaa aattgaagta gaagatttcc cggcgtttat cctggtcgat
     6241 gacaaaggta acgacttctt ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag
     6301 taacctcttc ggcccagcac ctggcagcat gctgccaggt gatccccctg ggccacctct
     6361 tttgcgattg taatttcatg cttgctggtg aatagtcagt attttccctg acttgcgaac
     6421 tcaccatgac ccgcacactc aagccgttaa ttcttaacac cggcgcactg gcgctaacgt
     6481 taatcctgat ttataccggc atttcggccc atgacaaact cacctggctg ctggaagtga
     6541 caccggtgat tatcgtcgtg ccgctactac ttgccaccgc cagacgttac ccgttaacgc
     6601 tgctgctcta tacgctcatc ttctttcacg ccatcatcct gatggtaggc gggcagtaca
     6661 cctacgcgaa agtccccgtt ggttttgagg tgcaggaatg gctggggttg agtcgtaatc
     6721 cgtatgacaa gctggggcac tttttccagg ggctggtgcc tgcactggtg gcacgagaaa
     6781 ttctcgttcg cgggatgtac gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg
     6841 tggcgctggc gataagcgcg atgtatgaat taatcgagtg gtgggcggca ctggcgatgg
     6901 gccagggagc ggatgatttt ctcggtaccc agggcgacca gtgggacacg caatccgata
     6961 tgttctgcgc gctgcttggc gcattaacga cagtgatatt gctcgctcgc tttcattgcc
     7021 gccagctacg gcgctatggc ttgataaccg gataacaacg tgcactttgt tgatgccgga
     7081 tgcggcgtga attccttatc cggtctacaa aatttgcaaa tccaaagcat tacattaaga
     7141 cgtggcctga taaacatatc gcatcaggcc attttcgtta gctattgtcc acgtcaggct
     7201 ttcacatcac tcactgcacc gaccggaata tcagcatgaa caggctcggg cggtactttg
     7261 cgatatttat ccagcagatg aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag
     7321 aaacggaagt agagaatcag cgttaccatg aaaaacagag tcggtagagc aatcataata
     7381 aactgcatac ctaatagggc ttgcgtagac tgttcaacat tcggtacata gccaatcatc
     7441 cctaacacaa ccgcaatgaa aaaagccgca aaggctgaac cgcccttcac caccatagtc
     7501 tgcacggagt aagcgatgct ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg
     7561 gtatctgcca ccatgatgac ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa
     7621 atacccgcaa tcacaatgag gacgacgttg tgatagctca ttaatgccat taacaggaga
     7681 acaccacagc ttaacaccgg aagaatagat gctccggccc ataaaatgcg tcgggataat
     7741 gatttaacta agcgtgggaa gaatactaac gtcaccaggt tagcagctcc cgcatacgac
     7801 agataatagg ggaacaaatc cgcatcaccg ataacatatg agaaataata gatagcaaat
     7861 ccggtaataa tgttgctggc tacattataa gcaagagcca tacccaagag gcatgaaagc
     7921 tgatcgtttt tataaataag cgcaacgatg gctttaagtg tcagatggct tccttcagca
     7981 gacgattgat tgtctgacga aaagacttca tgcacattgc gcagagtgat gatggttgaa
     8041 acaataaaaa aggcgatcaa taccagagtg aacatctgaa agccaaatcc ccgatcgcca
     8101 ccgccgacat aattaacaaa tggtagcgtc acacctgccg taacaaagcc tgccagactg
     8161 gcaaaaaaac gcggataagg aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt
     8221 ggaaccagcg accagaaggg aatatccata atggtgtaag tcatgcccca gaggatgcag
     8281 gtcacgcaaa caaagacaat ctgagtagta ccttcaaaca gatgcgcact aaagaggaga
     8341 aataagatta cagagtttgc caacgtaccg atcaggatcc agggtttgaa cttaccccat
     8401 cgcgatcgcg tagcatttac aatccatccc ataatcggat cgttaatagc atcccagatc
     8461 ctcgccacca gaaacaaagt accgaccaaa cccacagaca gcccgacgac atcggtgtag
     8521 taatacatga ggtacatata cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat
     8581 ccataactga gttttgtagt cattgaaatg ctcatagggt atcgggtcgc tgttaccaac
     8641 gcccgccttc tgttattggt atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag
     8701 tagcgtttag tcgcgtttta acggtgcaac cagcctggca gccagtcgcc gtgggcggca
     8761 atcaggtcgt caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg
     8821 tccatcatcg cggcgtggta aacacggtcg cgattttccg taagaatagc ttcggtcagc
     8881 agcgtctgta cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg
     8941 actttggtcg gctgaatgcc attagcatca accagacagg ctacttccac gcaacatcct
     9001 tgtggcaggt tatcaatcaa accatcgtta cggacgttgc cgtaaatcac actcggctcg
     9061 ccagtccaga tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc
     9121 cgggaggcgt ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag
     9181 cgtttcgggt actcatccag cggtacttta taacgctcaa tcaaatcctc acgacctagc
     9241 ttaataaacc acggcgtata ttcggcaaaa tgttctgacg attccgtgac gaagtagccc
     9301 agctttttga acatttcgta gcgcacaata ttctggcagc gagtattacc atgaatattt
     9361 ggcttcggtg cctgccctgc ttcataagcc gccagcagtt ccgggtagag attcacgtaa
     9421 ctgccgtcgg cggttttgcg ctccagctcc aggtaaaacg ccatatggtt gatacctgcg
     9481 caacggtaac gcagcgtagc cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt
     9541 ccctgcaccg aatggcacag cccgacctgt ttgatatacg gatagcgggc atacatcgcc
     9601 caggtattca tcgccattgg gttaacatag ttgagcatgg tggcatcggg gcagacttcc
     9661 gtcatgttct cgcaaatttg ccacagatgc ggaatggtac gtagcgcgcg cataataccg
     9721 cccggcccca acgtatcggc aatggtttgt tccagaccat gccgcttaca gacctcgaaa
     9781 tcagtcaccg tgcaaggttc ataaccgcca atctgaaatg ccaccacgac aaaatcggca
     9841 tcctctaagg cttctttctg ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa
     9901 tccatcagct tacgcaccac aatatgcgac tcttccaggc gggtggggtc aatgtccatc
     9961 agggcaatat gcgccgtttt cagcgcctcg cgatggaaca catcaccaag aatattttta
    10021 acgaaaatcg tcgaaccagc gccgataaat gtaattttgg gtgcagacat catgcagatc
    10081 tcctggcttg cttgaataac ttcatcatgg caggcgaatc tgcagaaaac ctccggcttc
    10141 caggttgagc attcccataa actcagatcc tgcgtgaagc agcagtaaat ctgagtttat
    10201 gggaataaat tacctgaaaa gtagagtttt cggcctcatg atgctcggac agtcccctcg
    10261 cccctctggg aagagggtga aaaccgtgct cccactcgca gtcatcctcc ctcactcctg
    10321 ccataattct gatattctag gaaagagagc catccatgaa tacagatacg tttatgtgca
    10381 gcagcgacga aaaacagacc cgcagcccgc tgtcgctgta ttcggaatac cagcggatgg
    10441 agattgagtt tcgcgcaccg catatcatgc ccaccagcca ctggcatggt caggtcgaag
    10501 tgaatgtgcc tttcgatggc gatgtggaat acctgatcaa caatgaaaaa gtgaatatca
    10561 atcagggtca tatcacgctg ttctgggcct gtacaccgca ccaactaaca gataccggaa
    10621 cctgtcagag catggcgatt tttaatctgc cgatgcatct gtttctctcc tggccgctgg
    10681 ataaagacct gattaaccac gtcactcacg gcatggtgat caaatcactt gccacacagc
    10741 aacttagccc gtttgaagta cgccgctggc agcaggaatt aaacagtccg aatgagcaaa
    10801 ttcgccagct cgccattgat gaaattggcc tgatgctcaa gcgatttagc ctctctggct
    10861 gggaaccgat tctggtcaat aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg
    10921 cgcaatttta tgttagccag atgctgggct ttattgccga aaactatgat caggcgctga
    10981 ccatcaacga tgtggctgag cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc
    11041 agcgggttat gcaattgacg atgaaacagt acattaccgc gatgcgcatc aaccacgttc
    11101 gcgcgttact gagcgatacc gataaaagta ttctcgatat tgccctgacg gcaggctttc
    11161 gttcaagtag tcgtttttac agcacgttcg gcaaatatgt cggcatgtcg ccgcaacaat
    11221 accgcaaact tagccaacag cgccgccaga cgtttcccgg ctaaagacga tatcagtatc
    11281 agccaaaaaa atagtttagc cgtcgataat aagcagtgaa aataatttaa aataatcaca
    11341 taacgtattg tttataaaac taaatatttc aaaaatgttt gtttttcacg cgctttacag
    11401 cccgaaaagg ccggaagata cttgcccgca acgaa
//
LOCUS       DADMEQ010000122        11183 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.123, whole genome shotgun sequence.
ACCESSION   DADMEQ010000122 DADMEQ010000000
VERSION     DADMEQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.123"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="JNZ05_003308"
     CDS             complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="JNZ05_003308"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HAY8566424.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFAWRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITP"
     gene            complement(721..1749)
                     /locus_tag="JNZ05_003309"
     CDS             complement(721..1749)
                     /locus_tag="JNZ05_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mal regulon transcriptional regulator MalI"
                     /protein_id="HAY8566425.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1924..3516
                     /locus_tag="JNZ05_003310"
     CDS             1924..3516
                     /locus_tag="JNZ05_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HAY8566426.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3526..4698
                     /gene="malY"
                     /locus_tag="JNZ05_003311"
     CDS             3526..4698
                     /gene="malY"
                     /locus_tag="JNZ05_003311"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HAY8566427.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4801..5802
                     /gene="add"
                     /locus_tag="JNZ05_003312"
     CDS             4801..5802
                     /gene="add"
                     /locus_tag="JNZ05_003312"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAY8566428.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5838..6878)
                     /locus_tag="JNZ05_003313"
     CDS             complement(5838..6878)
                     /locus_tag="JNZ05_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY8566429.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7121..7246
                     /gene="blr"
                     /locus_tag="JNZ05_003314"
     CDS             7121..7246
                     /gene="blr"
                     /locus_tag="JNZ05_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HAY8566430.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7519..7734
                     /gene="ydgT"
                     /locus_tag="JNZ05_003315"
     CDS             7519..7734
                     /gene="ydgT"
                     /locus_tag="JNZ05_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HAY8566431.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7820..8260
                     /locus_tag="JNZ05_003316"
     CDS             7820..8260
                     /locus_tag="JNZ05_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HAY8566432.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8337..8918
                     /gene="rsxA"
                     /locus_tag="JNZ05_003317"
     CDS             8337..8918
                     /gene="rsxA"
                     /locus_tag="JNZ05_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HAY8566433.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8918..9496
                     /gene="rsxB"
                     /locus_tag="JNZ05_003318"
     CDS             8918..9496
                     /gene="rsxB"
                     /locus_tag="JNZ05_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HAY8566434.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9489..>11183
                     /gene="rsxC"
                     /locus_tag="JNZ05_003319"
     CDS             9489..>11183
                     /gene="rsxC"
                     /locus_tag="JNZ05_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY8566435.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIAR"
BASE COUNT         2594 a         2884 c         2817 g         2888 t
ORIGIN      
        1 tggtgtaata acggatttca gggcatcggt taatattgcc cccggcgcaa tgccattcac
       61 ccgaatattt ttttcaccca ggtcaaatgc catatttctg accagatgac tggccgcagc
      121 tttagatgat gcataggaag tcatgtttat atttttattt tctgccgcca tagaagtgat
      181 ggtcagaata acgccaccgc catttttttc catttctggc gcaacaagtt gtgacagatg
      241 gaaaaaagaa aacacattca gttcataagc acggcgaaaa tctgccattg gcatatcaaa
      301 cggtttagga ccaccgccac cggcgttgtt aaccaggata tcaactttac ccagtttact
      361 gacggcaaag tctgccagtg cagagagttc ctgttcggaa gtaatatcac aacgccaggc
      421 aaatgcctga ccacccagtt gttgaatttc gtctacaaca tggttagctg cgtcggcgtt
      481 aatatcactg accaccacag atgcgccagc tgtcgcgaat gtaatggcga tttctttacc
      541 aatacctgca cccgcacctg tgatgatggc gcattttccg tcgagtctca ggttgtcaga
      601 attaaacaca taaacctcct gtgaacttca gtaaaaataa aaggcaccgc ctgtgtgcca
      661 cattttttat cattaacctt gaggaaagga cactaccaag tatagcagat aaagagagga
      721 ttatttcgcc gcaatgagcc gggcgggaat aataagattg cgtgaatgcg tctcttcatg
      781 ggtgattttt tgcatcatgc gatccgcaag tgtgatacca agttcccgcg ctggcgtgct
      841 ggcccaggta acaggtatat catcaagtgt ggttggtgtc gcatcggtaa atgccgccag
      901 cgaaacctgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc gccctgcttt
      961 cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca cggcactgat
     1021 ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct ggctggaagt
     1081 gcactccaac acccaatcgc tgtgaaacgg caggccaaat tttagtagag ttgcacaata
     1141 gccccccacc cgttctgcac gggttaatga ggaactttgc cctcccagcc aggcgatccg
     1201 ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca tgttgtccgg
     1261 gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca ccgggatagc
     1321 tttttcttct gccatccgcc gcaggtcatc gctgcttcct gcagccccgg caattaccac
     1381 accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct cgccgtcttt
     1441 accgccgtga agcaaaaaaa ccatccgtcc ctgcgcttcc agagcttccg tcaatccggc
     1501 cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa tgacgccgct
     1561 ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct cttcaatggc
     1621 ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccactaa gcaccagcga
     1681 aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta tttttttggc
     1741 ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt ttacctttta
     1801 tttatcttat acccgctatt atcgttgcgt aatgtgattt atgactcact aaaatttgat
     1861 aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac gaggagtcgt
     1921 tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca gcagttaggc
     1981 aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct cggcattggt
     2041 agttctctta gcagccatga tgtcataacc ctgatcccgg tcctgggcaa ccccgtgttg
     2101 caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt cctgcctgtc
     2161 atgttctgta tcgccatccc gctgggcctg gcacgcgaaa ataaaggcgt agcggcattc
     2221 gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt gaccaataaa
     2281 ggcattctgc caaccacaga tgccgccgtt ctgaaagcca ataacatcca gagcattctt
     2341 gggatccagt cgatcgatac cgggatcctc ggtgcggtga tcgccggtat tatcgtctgg
     2401 atgctgcatg agcgtttcca taatatccgc ctgccggatg cgctggcatt cttcggcggt
     2461 acgcgcttcg taccaattat ctcctcgctg gtgatgggcc ttgtcggcct ggtgattcca
     2521 ttagtctggc cgattttcgc catgggtatt agcggcttgg gccatatgat aaacagcgcg
     2581 ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttacc gtttggtctg
     2641 catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca ggaggtctgc
     2701 ggtcaaaccg tcagcggcgc actgaccatc ttccaggcgc aattgagttg cccgaccact
     2761 cacggttttt ctgaaagcgt cacgcgtttc ctttcgcaag gtaaaatgcc tgcgtttctc
     2821 ggcggtctgc caggtgcagc gttagctatg tatcactgcg cgcgcccgga aaatcgccat
     2881 aaaattaaag gtctgctgat ttctggcctg atcgcctgcg tcgttggcgg cactaccgaa
     2941 ccgctggaat tcctgttcct gttcgtagcg ccagttctgt atgtcatcca cgcgctgtta
     3001 accggcctcg gcttcaccgt catgtctgtg ctcggcgtca ccatcggtaa taccgacggc
     3061 aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa gtggtacatg
     3121 gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg tttcgctatc
     3181 acccgcttca atctgaaaac cccggggcgc gatagcgaag ttgccagctc aatcgaaaaa
     3241 gccgttgccg gtgcgccggg taaatcaggt tacaacgttc ctgcaatcct cgaagcatta
     3301 ggcggtgccg acaatattgt cagcctcgat aactgcatta cccgtctgcg tttgtctgtg
     3361 gaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat tggcgtagta
     3421 caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc agtaaaagat
     3481 gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt cgatttttca
     3541 aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc tgaccgtttc
     3601 ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac tgccccctgc
     3661 attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag ccgctggaaa
     3721 aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta caccgcaatt
     3781 gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga actgattcgt
     3841 cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga cgcattttac
     3901 aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa gcaggctgac
     3961 ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga atgtaaaatt
     4021 atgctcctgt gtaacccaca gaatcctacc gggaaagtgt ggacgtgcga tgagctggag
     4081 atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga aatccatatg
     4141 gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg gggagactgg
     4201 gcgttgctga cgtcgggatc gaaaagtttc aatattcccg cgctgaccgg tgcttacggg
     4261 attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg acgtgatggg
     4321 ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca gcaaggtgca
     4381 ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat ctcagataaa
     4441 atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta tctggcctgg
     4501 cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct tatcgagcaa
     4561 gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg ttttgtccgt
     4621 ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt aataaacgcc
     4681 atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa tcgttgcgca atcgtggatt
     4741 tttaccctgc tttgttttta taatggtgcg cacttttata tccagaaaaa gagtgcgacc
     4801 atgattgata ccaccctgcc attaactgat atccatcgcc accttgatgg caacattcgt
     4861 ccccagacca ttcttgaact tggccgccag tataatatct cgcttcctgc acaatccctt
     4921 gaaacactga ttccccacgt tcaggtcatt gccaacgaac ccgatctggt gagctttctg
     4981 accaaacttg actggggcgt taaagttctc gcctctcttg atgcctgccg ccgcgtggca
     5041 tttgaaaaca ttgaagatgc agcccgtaac ggcctgcgct atgtcgagct gcgtttttca
     5101 ccaggctaca tggcaatggc acataagctg cctgtagcgg gtgttgtcga agcggtgatc
     5161 gatggcgtac gtgaaggttg ccgcaccttt ggtgtgcagg cgaagcttat cggcattatg
     5221 agccgaacct tcggcgaagc cgcctgtcag caagagctgg aggccttttt agcccaccgt
     5281 gaccagatta ccgcacttga tttagctggt gatgaacttg gattcccggg aagtctgttc
     5341 ctttctcact tcaaccgcgc gcgtgatgcg ggctggcata ttaccgtcca tgcaggcgaa
     5401 gctgccgggc cggaaagcat ctggcaggcg attcgtgaac tgggtgcgga gcgtattgga
     5461 catggcgtaa aagccattga agatcgggcg ctgatggatt ttctcgccga gcaacaaatt
     5521 ggtattgaat cctgtctgac ctccaatatt cagaccagca ccgtagcaga gctggctgca
     5581 catccgctga aaacgttcct tgagcatggc attcgtgcca gcattaacac tgacgatccc
     5641 ggcgtacagg gagtggatat cattcacgaa tataccgttg ccgcgccagc tgctgggtta
     5701 tcccgtgagc aaatccgcca ggcacagatt aatggtctgg aaatggcttt cctcagcgca
     5761 gaggaaaaac gcgcactgcg agaaaaagtc gccgcgaagt aacaaaaatg gatggtgcaa
     5821 attgcaccat ccatttttca cgcaaggcac aacgtcgcgc gatgtttggc ggattcgatg
     5881 cccagctcaa tcaactccat tacctggatt gcctggcttg ccggaaccgg attttcacca
     5941 tcgccattta acgcatcacg aatcgccgca tagtaagccg gataattccc aggcaccgtc
     6001 aacagcgttt cttcgacacg ttcctcacct tccacgcggg taagtacgcc atcacgcata
     6061 tcgtagcccc agtcttcctg cggcagacgc tcgccatttt tcagacgttc ttcctgtgga
     6121 tcgaggccat atttcacata actgcctcgg gatccatgca cgatataccg tgctgactca
     6181 gcggctgcca gcatggtacc gtgtaaaatg actcgccgct gtggatagga caagatggcg
     6241 tggaaataat cggtcgactg cgctccgggc cgtaactgtg ccaaatctac cgtcatgctg
     6301 accggtaaac caaatagcgt aatggcctga tcaagaagat gcggtgctaa atcgtaccag
     6361 ataccgctgc ctgggccgcc ctgttcacgc caacgatcgc gcacctgcgg acggaagcgg
     6421 tcaaaatgag actcaaagta agcaacttca cccagcacgc cttccgcgag caaacctttt
     6481 agcgtcaaga aatcgctatc ccaacgacgg ttatggaata cagacagcac acgccccagg
     6541 ctttttgcca gcgcatccag ctctcgcgct tgtgacagtg tcacggtaaa gggtttatca
     6601 acgaccacat gtttacccgc ctcaagcgcc gctttggcta acgggaaatg ggtatcgttg
     6661 ggtgtaggaa tgacaatcag gtctatgttg ggatcgttaa acagatgctt cggctcagag
     6721 acaaccgtaa ccgttggcca gtcggctttt acttttgttt catcactgct ggagattacc
     6781 gccagttcca gcccgggcgt gcccgcaatc aggggcgcat ggaaggtttt gctcgcataa
     6841 ccatacccaa tcaacccaac acggatgttg tcgctcatgt cattgcctct cattgaagta
     6901 tgatggctat ttgacactat cctttaccca cgctcaacag tttaataacc tgccagcaat
     6961 aagggatgtt gtttaactta agtcaaaaaa atagcgaatt ttccaacgac aaaagctaaa
     7021 tatcgcaaaa acctcagtaa aaatcttgct ggagctatta ttgctaagta acatttaccc
     7081 cctgaagtta atggatcaat caagagagat gtgggctgta atgaatcgtc ttattgaatt
     7141 aacaggttgg atcgttcttg tcgtttcagt cattcttctt ggcgtggcga gtcacattga
     7201 caactatcag ccacctgaac agagtgcttc ggtacaacac aagtaagctc tgcacttgtg
     7261 gagcgacatg ctgcccgtcc gggtgcatgt tttcacttgt cggatattaa accaggaatt
     7321 tattatcttg ttcgatgttg ttggtgattg tcagggatag taaagcgcgt tactcttttc
     7381 agagcagttt ttcatccctg aatatttgcc atcagcgcat tcttattatt gtaataacac
     7441 aagcgttcac ttttggtgaa attttatcat cactaataat acctacccct ttggtattaa
     7501 ttattttatg gaccttttat gactgttcag gactacttat taaaatttcg caaaatcagt
     7561 tcactcgaaa gtctggaaaa actctacgac catcttaatt acaccctgac ggacgatcag
     7621 gaactgatca atatgtatcg tgctgccgat caccgtcgcg cagagctggt ttctggcggg
     7681 cgtttgtttg acctcggcca ggtaccgaag tccgtctggc actatgtcca ataaagaaag
     7741 tagcgatcgg ttataaaagc cttataataa cgcccctaaa attagatttg cctgtttgcg
     7801 tcgctattct ggagaaccta tgaccaccac aacgccacaa cgtattggag gctggttgct
     7861 tggcccgttg gcctggcttt tagtcgctct gttgagtacg acgctggcgt tgttgctgta
     7921 taccgctgcg ttatcttctc cgcaaacatt tcaaacgctc ggcggacaag cactgacgac
     7981 acaaatctta tggggcgttt cttttattac cgctatcgcc atgtggtatt acacactgtg
     8041 gttgaccatc gctttcttta aacgtcggcg ttgtgttcct aaacactata ttatctggct
     8101 gcttatttcc gtattgctgg cagtaaaagc attcgctttt tcacccgtgg aagttggcat
     8161 agccgttcgc cagttgctgt ttactttgtt ggcaaccgca ttgatcgtcc cctatttcaa
     8221 acgctcgtcg cgggtaaaag cgacgtttgt gaatccgtaa taaccttaca gttaacctgt
     8281 tgtcgcctgc tctggattaa cggataatag gcggcttttt tatttcaggc cgaaaaatga
     8341 ctgactacct gttactgttt gtcggaactg tactggtcaa taactttgta ctggtcaagt
     8401 ttctcggtct ctgtccgttt atgggggttt ccaaaaaact ggaaaccgcg atgggcatgg
     8461 ggctggcaac aacgtttgtg atgacgctga cgtctatttg cgcctggctt atcgatacgt
     8521 ggattttgat cccacttaat ctgatttacc tacgcaccct ggcatttatt ctggtgattg
     8581 ctgtggtcgt gcagttcacc gagatggtgg tgcgcaaaac cagcccggtg ctttaccgtc
     8641 tgctggggat ttttttgccg cttatcacca ccaactgtgc agtgctcggc gtggcgttgc
     8701 tgaatatcaa tctcgggcac aatttcttgc agtcggcgct gtacggtttt tccgccgctg
     8761 tcggtttctc gctggtgatg gtgctcttcg ccgccatccg cgaacgcctt gctgtggctg
     8821 atgtcccggc accttttcgc ggtaatgcca ttgcgttaat taccgcaggt cttatgtctc
     8881 tggcctttat gggctttagt ggtttggtga agttgtaatg aatgctatct ggattgccgt
     8941 tgccgccgtg agcctgctgg gcctggcgtt tggcgccatt ctgggttatg cctcccgccg
     9001 ttttgcggtg gaagacgatc cggtcgttga gaaaattgac gaaatcttac cgcagagcca
     9061 gtgtggtcag tgcggttatc ccggctgtcg cccctacgcg gaagccatca gctgtaacgg
     9121 tgaaaaaatc aaccgttgcg ccccaggtgg cgaagctgtg atgctaaaaa ttgccgagtt
     9181 gcttaatgtc gagccgcagc cgctggatgg cgaagcgcaa gagctaacgc ctgcgcggat
     9241 ggtggcggtt attgatgaaa ataactgtat tggctgcact aaatgtattc aggcttgtcc
     9301 ggtagacgcc atcgttggcg ctacccgtgc catgcatacg gtaatgagtg atctctgtac
     9361 gggctgcaat ttatgtgttg atccgtgccc gacgcactgc atctcgttgc aaccggtcgc
     9421 agaaacacct gactcctgga aatgggatct gaacaccatt cccgtgcgta tcattcccgt
     9481 ggaacaccat gcttaagtta ttctctgcat tcagaaaaaa taaaatctgg gatttcaacg
     9541 gcggcatcca tccaccggag atgaaaaccc agtccaacgg tacccccctg cgccaggtac
     9601 cactggcgca gcgttttgtt attccactga aacagcatat tggcgctgaa ggtgagttgt
     9661 gcgttagcgt cggcgataaa gtattgcgcg gccagccgct tacccgtggt cgcggcaaaa
     9721 tgctgcctgt tcacgcgccc acctcgggta ccgttacggc tattgcgccc cactctacgg
     9781 ctcatccttc agctttagct gaattaagcg tgattattga tgccgatggt gaagactgct
     9841 ggatcccgcg cgacggctgg gtcgattatc gctctcgcag tcgcgaagag ttaatcgagc
     9901 gcatacatca gtttggtgtt gccgggctgg gcggtgccgg attcccgaca ggcgttaaat
     9961 tgcagggtgg tggagataag attgaaacgt tgattatcaa cgctgctgag tgcgagccgt
    10021 acattaccgc cgatgaccgt ttgatgcagg attgcgcggc tcaggtcgta gagggtattc
    10081 gcattcttgc gcatattctg cagccacgcg aaattcttat cggcattgaa gataacaaac
    10141 cgcaggcgat ttccatgctg cgcgcggtgc tggcggactc taacgatatt tctctgcggg
    10201 tgattccaac caaatatcct tctggcggtg ctaaacaatt aacctacatt ctgaccggga
    10261 agcaggttcc acatggcggg cgttcatccg atatcggcgt attaatgcaa aacgtcggca
    10321 ctgcttatgc agtgaaacgt gccgttattg atggcgagcc gattaccgag cgtgttgtaa
    10381 ccctgactgg cgaagcaatc gctcgcccgg gcaacgtctg ggcacggctg ggaacgccag
    10441 tgcgtcattt attgaatgat gccggattct gcccctctgc cgatcaaatg gtgattatgg
    10501 gtggcccgct aatgggcttt accttgccat ggctggatgt cccggtcgta aagattacca
    10561 actgtctgtt ggctccctct gccaatgaac ttggcgaacc acaggaagaa caaagctgca
    10621 tccggtgtag cgcctgtgct gacgcctgcc ctgtggatct tttgccgcaa cagttgtact
    10681 ggttcagcaa aggtcagcaa cacgataaag ctaccacgca taacattgcc gactgcattg
    10741 aatgtggggc ttgcgcgtgg gtttgcccga gcaatattcc cttggtgcaa tatttccgtc
    10801 aggaaaaagc tgaaattgcg gctattcgtc aggaagaaaa gcgcgccgca gaagccaaag
    10861 cgcgtttcga agcgcgccag gctcgtctgg agcgcgaaaa agctgctcgc cttgaacgac
    10921 ataagagcgc agccgttcaa cctgcagcca aagataaaga tgcgattgct gccgctctgg
    10981 cgcgggtgaa agagaaacag gcccaggcta cacagcctat tgtgattaaa gcgggcgaac
    11041 gcccggataa cagtgcaatt attgcagcac gggaagcccg taaagcgcaa gccagagcga
    11101 aacaggcaga actggagcaa actaacgacg cagcaaccgt tgctgaacca cgtaaaactg
    11161 ccgttgaagc agctatcgcc cgc
//
LOCUS       DADMEQ010000123        11133 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.124, whole genome shotgun sequence.
ACCESSION   DADMEQ010000123 DADMEQ010000000
VERSION     DADMEQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11133
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.124"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(194..1447)
                     /locus_tag="JNZ05_003320"
                     /pseudo
     CDS             complement(194..1447)
                     /locus_tag="JNZ05_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1554..2447
                     /locus_tag="JNZ05_003321"
     CDS             1554..2447
                     /locus_tag="JNZ05_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8566436.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYLDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            2582..3802
                     /gene="mlc"
                     /locus_tag="JNZ05_003322"
                     /gene_synonym="dgsA"
     CDS             2582..3802
                     /gene="mlc"
                     /locus_tag="JNZ05_003322"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HAY8566437.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            3927..4622
                     /gene="bioD"
                     /locus_tag="JNZ05_003323"
     CDS             3927..4622
                     /gene="bioD"
                     /locus_tag="JNZ05_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY8566438.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4575..5855)
                     /gene="clcB"
                     /locus_tag="JNZ05_003324"
     CDS             complement(4575..5855)
                     /gene="clcB"
                     /locus_tag="JNZ05_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HAY8566439.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYKVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGGLIVG
                     LLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVFTPTL
                     FIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEMTGEY
                     QLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6014..6628)
                     /gene="dmsD"
                     /locus_tag="JNZ05_003325"
     CDS             complement(6014..6628)
                     /gene="dmsD"
                     /locus_tag="JNZ05_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HAY8566440.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6671..7525)
                     /locus_tag="JNZ05_003326"
     CDS             complement(6671..7525)
                     /locus_tag="JNZ05_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAY8566441.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7527..8144)
                     /gene="dmsB"
                     /locus_tag="JNZ05_003327"
     CDS             complement(7527..8144)
                     /gene="dmsB"
                     /locus_tag="JNZ05_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAY8566442.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDNPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(8155..9240)
                     /locus_tag="JNZ05_003328"
     CDS             complement(8155..9240)
                     /locus_tag="JNZ05_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005022811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY8566443.1"
                     /translation="MTATRDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQ
                     DDSKCEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSA
                     KFERKPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKT
                     TGIFKKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIH
                     PIPAYTSGFDGWDAPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDA
                     QARGIRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSI
                     NILTSHRPSPLAKGNPSHSNLVQIEKV"
     gene            9479..10204
                     /locus_tag="JNZ05_003329"
     CDS             9479..10204
                     /locus_tag="JNZ05_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566444.1"
                     /translation="MNTNEVSVINLDQLVSMSSYDFLTKVINPARVEAGEKPVRNNDF
                     VSRIEDELDGQNVAYEIFVSFGQDVKSYTLNTDQLLLVGMRESKAVRRKVLAYTGATP
                     TEVIKALDLKLTSSKMLTASVLTGYLKTLCRPNPFYTKRVTEFGTTDIDLSTGRPRAI
                     FNKKGETNGYYRLQPQPNDKEPTIKILRKGYDWLKDNWKDIAHDMRTSNIKVVRRLVD
                     SHLNGKATKSQKAAAGKGSDYGF"
     gene            10451..10672
                     /locus_tag="JNZ05_003330"
     CDS             10451..10672
                     /locus_tag="JNZ05_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566445.1"
                     /translation="MTTKQHIDPRTPIGKATLRYRGLPTRHLLSMLGMGVEDPERPFY
                     SRDELIDLLVDRDLNNQLRRAFAKLDATH"
     gene            10774..>11133
                     /locus_tag="JNZ05_003331"
     CDS             10774..>11133
                     /locus_tag="JNZ05_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566446.1"
                     /translation="MSSKASALFNNNDWKLHVTDINLYENTVSLDGQSYPLSFAIKTL
                     IPGYLSGLPATSRESMELLEALAEAGVTIGNFFSNDLMTAYQRRQQNKRAEAERIAKE
                     QRIQAERMREENMTDAEW"
BASE COUNT         2900 a         2832 c         2858 g         2543 t
ORIGIN      
        1 tagaataact gcgcatcagt aataaatgac acacagcaaa atgaatccgt ttatttgggt
       61 acttataatc ctgatgacgc tagatgcgct gcgggaattg gctggcgctt cgtctatttt
      121 aggatggcta ttaacgttgg tttgagcagg caataagtcc ggacgggtat ttaccgcagt
      181 ccggacttat ttttcaggcg tgcagacgac gatgcaaacg cgccccgacc agcagagcaa
      241 tgaccagcat cagagcaata aatgcgccga cgccgttcca gccatagtta tgccagaaaa
      301 caccacccag cgtcccggca atactcgacc ccagatagta actgaacaga tacagcgagg
      361 aggcctggcc tttagcgcgt tttgcgcggg ggccgatcca gctgctggct actgagtggg
      421 ctgcgaagaa tccggctgag aagagtaaca ttccggcaaa gatcagccac agcgagctaa
      481 ataaggtcat cagtaaacca aacggcataa ctcccgtcga aaacaacatc accggaccgc
      541 gcccatagcg ggtggtcatg gttccggctt tgggtgagct ccatgtaccg gtcaaataag
      601 ccagcgataa taagccaacc acggcctgac tgacatgcta gggggaaagc atcaaccgat
      661 agccgatgta attaaacagc gtgacgaacg accccatcag caaaaagcct tctgcgaaca
      721 ataacggtaa tccccggtca cgccagtgca gacgaaagtt gataaataac gtcttagggc
      781 gcagcgaagt cgggcaaaaa tggcgtgatt cagggaggat tttccagaac atcaacgcct
      841 aggccagcgc gaaacaaccg attgccgcca gagcaattcg ccagttgaaa aagtccgtga
      901 agacaccact aattaagcgt ccgctcatgc cgccaattga gttgccgctg atatacaacc
      961 ccatcgaaaa ggccacgaaa ctggggtgga tttcctcgct aagataagtc atgccaacag
     1021 ctgccacgcc acttaacgaa agcccaatca aggcgcgcat aatcaaaatg ccgtgccagc
     1081 tggtcatcat tgtcgaaagt aacgtacaaa tggaggccaa cagtagcgcc gtgaccatca
     1141 ctggtttgcg accaatggca tcggatagcg ggccagtaaa tagcaaaccg atagccaaca
     1201 tcgccgtgga aatggacagt gaaatactac tgttcgcggg cgttaagcca aactcctgcg
     1261 aaagcaccgg aaggataggc tgcacacaat agagaagtgc aaaggttgcc agtccggcag
     1321 agaacagcgc cagggtgacg cgcataaatt gcggcgtacc gcgtttaata aattgatttg
     1381 gctgagaaat gctttgcttg tcagtgtcgc ttgccggagc gccatcaaca gttgtagtac
     1441 ggctcacttg aaatccttgc taaatatgcc tgtggatcag gcttatacat agggtaggaa
     1501 aatcgaattg ttctgtctaa tatattaata atctcaaata agatgtttta aatatgaata
     1561 ttgaacttcg tcatctgcgt tactttgttg ctgttgcgga agagctgcat tttgggcgcg
     1621 cggctgcccg cctgaatatt tcgcaaccgc cgctaagtca gcagattcag gcactggagc
     1681 aacaaatcgg tgcccgactg ctggcacgaa ctaatcgcag tgtattgctg acggcagcag
     1741 gaaaacagtt tcttgcagat agtcggcaaa tcctgtctat ggtggatgac gctgccgctc
     1801 gcgctgaaag actgcatcag ggtgaagcgg gggagttgcg tattggtttt acttcgtccg
     1861 ctccttttat tcgggcggtg tccgatacgt tatcgctgtt tcgccgtgat tatcttgatg
     1921 tccatttaca aacccgcgaa atgaacactc gcgagcaaat cgctccgctc attgaaggaa
     1981 cgctggatat gggattgctg cgtaacacag cgttaccgga gacgcttgaa cacgcagtca
     2041 tcgtccatga accgcttatg gcgatgatcc cgcacgatca tcccctggca aataatccga
     2101 gtgtaacact ggctgaactg gcgaaagaac cctttgtctt ttttgatccg cacgtcggga
     2161 ctgggctgta tgacgatatt ctcggactga tgcgacgtta ccatttgacg cccgtcatca
     2221 ctcaggaggt gggcgaggca atgaccatca tcggtctggt ttccgccggt ctgggtgttt
     2281 caattttgcc tgcgtcattt aaacgtgttc agctcaacga aatgcgctgg gtgccgattg
     2341 ctgaagagga tgcggtttct gaaatgtggt tggtctggcc gaaacatcat gaacaaagtc
     2401 cagctgcgcg taactttcgt attcatctgc tgaatgctct caggtgaggg aaatttcagc
     2461 gaaaaagctc gaaaaatgtg ctgttaatca catgcctaag taaaaatttg acgacacgta
     2521 ttgaagtgct tcaccatagc ctacagatta tttcggagcg cgaaaatata gggagtatgc
     2581 ggtggttgct gaaaaccagc ctgggcacat tgatcaaata aagcagacca acgcgggcgc
     2641 ggtttatcgc ctgattgatc agcttggtcc agtctcgcgt atcgatcttt cccgtctggc
     2701 gcagctggct cctgccagta tcactaaaat tgtccgtgag atgctcgaag cacacctggt
     2761 gcgagagctg gaaatcaaag aagcggggaa ccgtggccgt ccggcggtgg ggctggtggt
     2821 tgaaactgaa gcctggcact atctttctct gcgcattagt cgcggggaga ttttccttgc
     2881 tctgcgcgat ctgagcagca aactggtggt ggaagagtcg caggaactgg cgttaaaaga
     2941 tgacttgcca ttgctggatc gtatcatttc ccatatcgat cagtttttta tccgccacca
     3001 gaaaaaactt gagcgtctaa cttcgattgc cataaccctg ccgggaatta ttgatacgga
     3061 aaatggtatt gtacatcgca tgccgttcta cgaggatgta aaagagatgc cgctcggcga
     3121 ggcgctggag cagcataccg gcgtaccggt ttatattcag catgatatca gcgcatggac
     3181 gatggcagag gccttgtttg gtgcctcacg cggggcgcgc gatgtgattc aggtggttat
     3241 cgatcacaac gtgggggtgg gcgtcattac cgatggtcat ctgctacacg caggcagcag
     3301 tagtctcgtg gaaataggcc acacacaggt cgacccatat ggtaaacgct gttattgcgg
     3361 gaaccacggc tgcctcgaaa ccatcgccag tgtggacagt attcttgagc tggcacagct
     3421 gcgtctcaat caatccatga gctcgatgtt acatgggcaa ccgttaaccg tggactcatt
     3481 gtgtcaggcg gcattgcgcg gcgatctact ggcaaaagac atcattatcg gggtgggcgc
     3541 gcatgtaggg cgcattcttg ccatcatggt gaatttattt aacccacaaa aaatactgat
     3601 tggctcaccg ttaagtaaag cggcagatat cctcttcccg gtcatctcag acagcatccg
     3661 tcagcaggcc cttcctgcgt atagtcagca cattagcgtt gagagtactc agttttctaa
     3721 ccagggcacg atggcaggcg ctgcactggt aaaagacgcg atgtataacg gttctttgtt
     3781 gattcgtctg ttgcagggtt aacatttttt aactgttcta ccaaaatttg cgctatctca
     3841 atttgggcca ggaaagcata acttagactt tcaaggttaa ttattttcct agtttatatt
     3901 tgtgaagcat aacggtggag ttagtgatgc tgaagcgttt ctttattacc ggtacagaca
     3961 cttctgtagg gaaaacggtg gtttcccgcg cattgctaca agcgttagcc tcccagggaa
     4021 aaacggttgc gggatataaa cccgtagcga aggggagcaa agagacaccc gaagggctgc
     4081 gtaataaaga tgccttggtg ttgcagagtg tttcaaccat cgaactgcct tatgaagcag
     4141 ttaatcctat cgcgttaagc gaagaagaaa gtagcgtggc gcacagttgc ccaatcaatt
     4201 acaccctcat ttcaaacggc ctggcaaacc tgaccgataa agtcgatcat gtcgtggtag
     4261 aagggactgg cggctggcgc agtctgatga atgatttgcg tccactctct gaatgggtag
     4321 tgcaggaaca actgccggtg ttgatggttg tcggtattca ggaaggttgc attaaccatg
     4381 cactgctaac agctcaggcg atcgccaacg acgggctgcc gctcattggc tgggtggcta
     4441 accgaatcaa cccaggactg gcgcattatg cggaaatcat tgatgtgctg ggtaaaaaac
     4501 ttccggcacc gctcattggt gaactgcctt atctgccgcg cgccgaacag cgtgaactgg
     4561 ggcaatacat ccgcttagct atgctgcgca gtgtgctggc ggtagataga gtcacggtgt
     4621 aacgtccgcg aaattaccga cgcaattacg caggcaatca ataaaccggg gagtagctga
     4681 tactccccgg tcatttcaca tatcatcaac gtcgacataa tcggcgcgtg cgtggttgcc
     4741 gccaacagtg tcgccatccc ggtcaatccg agtaaaagcg taatttcttc gccatcaggg
     4801 aaccataatc ccaggctacg accatacaac atgccaatgg caagaccgat aaatagcgtc
     4861 ggtgtaaaga caccaccggg tgcgccggaa ccgctactcg ccagcacggc acacagttta
     4921 caaaggaaga tcccggcaat gatcattaac agtggtgggg cggttaaaaa ggattgtacg
     4981 gtgctatagc cgttgcccca cactgcaggt gtgaacaggg aaagcagacc cacgatcaac
     5041 ccgcctagtg ccagttgcca gggcggcgca agtttgagac tcacaaatcc acgatgacag
     5101 gcgttcatta acgttaacaa cagtggtccg cacagacctg ccagcacacc tgtactgata
     5161 atcagcgcat agtcacgagc ctgaaccgtc actttgtaga gtaacgcgtc gctatgatta
     5221 atcagattgc taaccagcaa tgccacgacg gcggaaataa tcaccgggcc gagagaggcc
     5281 aacatcatag tgccaaacag cacttcggct ataaataaac tgccagcaag cggggcacga
     5341 taggccgcag ccattcccgc cgcggcccca caggcgatcc ataatttcca ttcctggcgt
     5401 ggcgtaaaac gttgggcaaa acaggaggcg gcaagggcag ctaaaagaat catcgcacct
     5461 tcgcgaccaa ttgcactgcc gctggttact accagcagcg aggcaagcga tttaaccagg
     5521 cttgctgcgt aatcgaactg tccatcggtt tgcaacgctt ccatgtaatc ggtcggcgca
     5581 tgagggcgtt gttgggtaaa tttctgccag cccatcagca acaaacccgc cgccagtccg
     5641 ccgagcgccg gagttagcag ccgtcgccag ggggaaaggt ttgtcgctgc attgaccaga
     5701 ctgccggagt cattattgag gaacaaccac tccagtagca gcatcgcatg acgaaacccg
     5761 gcaacggcaa agacagcgag aataccgacg actgttgcga taagcagacg gcggaacata
     5821 gtgcgcagat ccggatagat gtgtagatga tgcatgagca gaaatgggca atgaaacacg
     5881 ggcgactatt ttgcggcaaa agaggccaac cgcaaaagca gtgcgtgctt taagagataa
     5941 aaaaacgtga cattgtaacg tattattgcc gaatcgcgcg taatcgtcac catccggcaa
     6001 tattacggtg atcctatcga aagagtggtt taaccgcgac aggaatcaac agttgtgatt
     6061 gccattgcgc cagcgttaag cgagccagtt cacccagtgc acggtagaaa gggtgttctg
     6121 ctttttcgat aaaaacatca agaaaacgtg ttgaccacgg aaaaaggtgc catgccagca
     6181 attcttcgca ttctgtctgg cgaccattct ccgccaacca cgcagccatc aacagcagtg
     6241 aaccaaaatg atcttccggt tcgttttgct tcatttcaaa ctgaatgcct ttctcgcgca
     6301 tccactgacg aagtgccaat gttgaatcgc caaacagcac agattcgcga tccagccaga
     6361 ccgaccccca cggcggagac ggcagtgccc acgggccgac aaacaaacgc tgccaggcct
     6421 gggcgtgagt ctcttcacac tgtgtctgaa aagccgtcac cagcggcgct aatgacgctt
     6481 ctggtagagg ccactgagtt tcccagccgt cactggtgag taccgcaacc agaggtgcgg
     6541 cttccgcact ctctggagcg taataaaaca gcgctcccaa cacgcgcgcc gcgacagaaa
     6601 aattatcttg ctgtgaaaaa tgggtcattc ctaacatcct gaaatgtgcg ggttgccccg
     6661 caccaatttg ttaacctgca atagccattc ctactgtcat gtgcaggcca taaaagagca
     6721 cacgcccgat catctcacca acaagtatga ggattagccc cagaacaagt ccggcaacgt
     6781 gaggttcacg gcgacgaatg agcggacaaa gccagcatcc aagacctgcg cataacaata
     6841 ccacacgcca gacctgtaag gaggcgtaat ctggtaccag tgcgctggct tgttgcacgg
     6901 atgagtgaat agacgccaga gacaaacctt gcagcacaat cacctcggca caagcgatca
     6961 gcgccagaac gctaatgatg gcaaatggtg tggtattaaa tgtaacgcgg gctgcccgca
     7021 gaattgcggc agcaagtatt ggaccgctca acaataccgt caggaagaaa gccagcgtag
     7081 tgtaaccgtt atgccaggtt ggcacggtgt cgatttgata cacgcaggtc atcatccaga
     7141 cgaaaatgac gccgagcgcc atgctgacaa gtaaccagag tttccccaac gcttgcggca
     7201 ttttaccgat gaccgccacc agccaccata gtccgccaac ggcaaaaaat atggaaccgg
     7261 cagcaatctc gttactcaga ccagaagctc cgattcgatt cagcgagtta aacgctcgca
     7321 gtggcgatcc caggtgcatg acagaggcaa taaacccgat gcccatcaac agccagagga
     7381 aaaacatgcc acgaacaatg tgctgacggt ctgcgtcatt tttggcggcg aaccagccaa
     7441 ttccgctaac aatcagcgcg cctactacgc attgacccaa tacggtaaag ataaccagtg
     7501 gccattcatg ccatccattt cccattttac acttcctccg gattagccag ataaccagtg
     7561 gtatcgccgg tcgggcggct gttggcgtta ggtttgataa cgatattggg ttttgtgaaa
     7621 tgcgcgcgcg gcagtggcgc gacggcagcc agagtaccgt gcttctggcg cagctcttca
     7681 attggaccga actcaagtgc acgcagcggg caggattcga cgcatatggg ttgtttcccc
     7741 tcggcgacgc gcgaataaca accatcgcac ttcgtcatgt gccctttttc agcattgtac
     7801 tgtggtgcgc cgtacggaca agccatgtgg cagtagcggc agccgataca aacatcttca
     7861 tcaaccacca caaagccatc ttcacgctta tgcattgcgc cgctggggca gacttttgta
     7921 catgcggggt tatcgcaatg gttacaggag atggagagat aataagcgaa cacgttctgg
     7981 tgccagatgc cattatcttc ctgccagtcg ccgccagcat attcataaat acggcggaaa
     8041 ctgacttccg ggccaagatc tttaaaatct ttgcacgcca gttcgcaggt tttacagcca
     8101 gtacagcggc tggaatcgat aaaaaatcca tattgtgtgg tcatgggcta ctccttaaac
     8161 cttttcgatc tggacaagat tgctgtgcga cgggtttccc tttgctagcg gtgaagggcg
     8221 gtgagaggtc agaatattga tactgccgcc gtgatcgacc cggtcaccaa acatatccgc
     8281 tttaagccac gcaccttgcc cgatggcggt aacgccaggc agaatacgcg gagtcacttt
     8341 tgcggcaatc agcatttctc cattattgtt aaatacccgc acggtatcgc catgacggat
     8401 accgcgtgcc tgagcatcaa tggggttgat ccacacctct tgtgggcagg cctgctgtaa
     8461 cacatcaata ttgccgtagc tggagtgggt acgcgctttg tagtggaagc ccgttaactg
     8521 cagtggatag gttttccgca ggggagcgtc ccagccatca aaacctgagg tatacgcagg
     8581 aatgggatga ataatttcat cttttttcaa ttcccaggta tctgcaatct tcgccagtcg
     8641 ttcagaataa atttcgattt tccccgaagg tgttttcaac gggtttgcct gtggatcttc
     8701 acggaatgcg cggaaagcga cgtagtgttc ttccgggcat tttttcttaa agatcccggt
     8761 cgttttcatc tcctcgtagt cgggcatctc agggttacgt tccttcgttt tcgcatggag
     8821 atatttgatc cattcatgct gactgcgacc ttcagtaaag gtttgataaa cgtctggtcc
     8881 taagcgtttg gcgacttcac tcagcatcca gtagatgggt ttgcgttcaa attttgctga
     8941 ggttgcgggt tgggcgagga tcacatagcc catattccct gcagattcat gagagataag
     9001 gtcttcttgc tctgttggca tcaggtcagg caacaggata tcgcaatact tagccgaggc
     9061 cgtcatgaag tggtcaatgc caacaatcat ctcgcacttg ctgtcatcct gaagcacctc
     9121 atgggtgtga ttgatgtcgc catgttgatt gatcaatgtg ttactggcgt agcaccataa
     9181 aaacttgatg gggacatcca gtttttcttt tccacgaaca ccatcacggg tcgcggtcat
     9241 tttcgtacca tggtcgatgg catctgtcca tgtaaacatc tggggtcctt acagctcgtc
     9301 ctgacatgcg gtctcgcctt atatgcacat ggagaacatg gtcactccag accaacacta
     9361 atcaacatta accctattca ctttcgttaa gcactttttg gtgggtccgt ttggatgcat
     9421 tgaataggtg cggactccac atggagacag aaagtgcttt accaaaggag catcctttat
     9481 gaataccaac gaagttagcg tcatcaacct tgaccagctt gtgtctatga gtagctacga
     9541 tttcttgact aaggtcatca acccggctcg tgtggaggct ggggagaagc ctgtccgtaa
     9601 caacgacttt gtgtcccgta ttgaagatga gctggacggt caaaatgtag cctacgaaat
     9661 tttcgtaagc ttcgggcagg acgttaaaag ctataccctc aacactgacc agcttctact
     9721 ggtcgggatg cgtgagtcta aagctgtgcg tcgtaaggtc ctagcctata caggcgccac
     9781 gcctaccgag gttattaaag ctctggacct taagttgacc agctctaaga tgctgacagc
     9841 tagcgtcctg actggatatc tgaagaccct gtgtcgtcct aacccattct acaccaagcg
     9901 tgttactgag tttggcacta cggatataga cctatcgacc ggacgtccac gagccatctt
     9961 caataagaaa ggtgagacca acggctacta tcgtctacag ccacagccta acgacaaaga
    10021 gcctaccatc aagattctac gcaagggtta tgactggctg aaggataact ggaaggatat
    10081 cgctcatgac atgcgcacat ccaatatcaa ggttgtccgt cgtctggtgg attctcacct
    10141 gaatggtaag gctacaaagt cccagaaagc agcagcgggc aaggggtctg actatgggtt
    10201 ctaagcgact cactgatgaa ctgattatgg agtcaatagc gacaaccata tgtttcattg
    10261 agagacacgg ccctgagcat cctgcggacc ctatcttact gacttctctg cttcggcata
    10321 tacacgaaga gatggcactg agagtcgacg agcgactgcc tctagagata ccagagggtc
    10381 tacctaactt ccaagctcct atcaccagtg aatttgacgt ggatgattgg tctaacgagg
    10441 acttctaaca atgacaacta aacaacatat cgacccacgg acacccatcg gaaaggctac
    10501 tctccgttac cgtggtctgc ctacccgtca cctcctgtca atgctgggga tgggcgtaga
    10561 ggaccctgag cgtcctttct atagtcgaga cgaactgata gacctgctgg tcgaccgtga
    10621 cctgaacaat cagttacgcc gagcgttcgc taagctggac gccactcact aacccctaat
    10681 ttaaaccttt tgagatgacc gatgcgggga gacccgtagg cataactgtg tctgcggtac
    10741 cgatagtctc aactctcaag aggagtaatt actatgtcta gcaaagctag tgcgttattc
    10801 aacaacaatg actggaaact ccatgtcacc gacatcaatc tctacgaaaa cactgtgagt
    10861 ctggacggtc agtcctaccc actgtccttc gctattaaga ccctgattcc gggctacctg
    10921 tcaggactgc ctgccaccag ccgcgagtcc atggaattac tggaagctct ggctgaagct
    10981 ggcgtgacca ttggaaactt cttcagcaat gacctgatga ccgcctacca acgtcgccag
    11041 cagaacaagc gagccgaggc tgagcgtatc gctaaagagc agcgcattca ggctgagcgt
    11101 atgcgtgaag agaacatgac cgatgcagag tgg
//
LOCUS       DADMEQ010000124        11093 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.125, whole genome shotgun sequence.
ACCESSION   DADMEQ010000124 DADMEQ010000000
VERSION     DADMEQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11093
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.125"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            215..2920
                     /gene="malT"
                     /locus_tag="JNZ05_003332"
     CDS             215..2920
                     /gene="malT"
                     /locus_tag="JNZ05_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HAY8566447.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWNLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALVQQTEQIARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(2963..3769)
                     /gene="rtcA"
                     /locus_tag="JNZ05_003333"
     CDS             complement(2963..3769)
                     /gene="rtcA"
                     /locus_tag="JNZ05_003333"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HAY8566448.1"
                     /translation="MGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVLQTVLPALWFADG
                     PSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFYPAGGGVVATEVS
                     PVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSLHEQNIHNLPRDQ
                     GPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLASPAAVGEYLADQL
                     VLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTRVSIE"
     gene            complement(3844..5070)
                     /locus_tag="JNZ05_003334"
     CDS             complement(3844..5070)
                     /locus_tag="JNZ05_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HAY8566449.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            5259..6857
                     /locus_tag="JNZ05_003335"
     CDS             5259..6857
                     /locus_tag="JNZ05_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
                     /protein_id="HAY8566450.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGAQESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(6839..7597)
                     /locus_tag="JNZ05_003336"
     CDS             complement(6839..7597)
                     /locus_tag="JNZ05_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HAY8566451.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(7614..8444)
                     /gene="glpG"
                     /locus_tag="JNZ05_003337"
     CDS             complement(7614..8444)
                     /gene="glpG"
                     /locus_tag="JNZ05_003337"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HAY8566452.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(8489..8815)
                     /gene="glpE"
                     /locus_tag="JNZ05_003338"
     CDS             complement(8489..8815)
                     /gene="glpE"
                     /locus_tag="JNZ05_003338"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HAY8566453.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            9005..10510
                     /gene="glpD"
                     /locus_tag="JNZ05_003339"
     CDS             9005..10510
                     /gene="glpD"
                     /locus_tag="JNZ05_003339"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAY8566454.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSIGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(10779..11009)
                     /locus_tag="JNZ05_003340"
                     /pseudo
     CDS             complement(10779..11009)
                     /locus_tag="JNZ05_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         2680 a         3035 c         2841 g         2537 t
ORIGIN      
        1 gatgaacata agggaaacca gtattcacgc tggatcagcg tcgttttagg tgagttgtta
       61 ataaagattt ggaattgtga cacagtgcaa attcagacac ataaaaaaac gtcatcgctt
      121 gcattagaaa ggtttctggc cgaccttata accattaatt acgaagcgca aaaaaaataa
      181 tatttcctca ttttccacag tgaagtgatt aactatgctg attccgtcaa aactaagtcg
      241 tccggttcga ctcgaccata ccgtggttcg tgagcgcctg ctggctaaac tttccggcgc
      301 gaacaacttc cggctggcgc tgatcacgag tcctgcgggc tacggaaaga ccaccctcat
      361 ttcccagtgg gcggcaggca aaaacgatat cggctggtac tcgctggatg aaggtgataa
      421 ccagcaagag cgtttcgcca gctatctcat tgccgccgtg cagcaggcaa ccaacggtca
      481 ctgtgcgata tgtgagacga tggcgcaaaa acggcaatat gccagcctga cgtcactctt
      541 cgcccagctt ttcattgagc tggcggaatg gcatagccca ctttatctgg tcatcgatga
      601 ctatcatctg atcactaatc cagtgatcca cgagtcaatg cgcttcttta ttcgccatca
      661 accagaaaat ctcaccctgg tggtgttgtc acgcaacctt ccgcaactgg gcattgccaa
      721 tctgcgtgtt cgtgaccaac tgctggaaat tggtagtcag caactggcat ttacccatca
      781 ggaagcgaag cagttttttg attgccgtct gtcatcgccg attgaagccg cagaaagcag
      841 tcggatttgc gatgacgttt ccggttgggc gacggcacta cagctaatcg ccctctccgc
      901 ccggcagaat acccactcag cccataagtc ggcacgccgc ctggcgggaa tcaatgccag
      961 ccatctttcg gattatctgg tcgatgaggt tttggataac gtcgatctcg caacgcgcca
     1021 ttttctgttg aaaagcgcca ttttgcgctc aatgaacgat gccctcatca cccgtgtgac
     1081 cggcgaagaa aacgggcaaa tgcgtctcga agagattgag cgtcaggggc tgtttttaca
     1141 gcggatggat gataccggcg agtggttctg ctatcacccg ctgtttggca acttcctgcg
     1201 ccagcgctgc cagtgggaac tggcggcgga gctgccggaa atccaccgtg ccgccgcaga
     1261 aagctggatg gcccagggat ttcccagcga agcgattcat catgcgctgg cggcaggcga
     1321 tgcgctgatg ctgcgcgata ttctgcttaa tcacgcctgg aatttgttca accatagcga
     1381 actgtcgctg ctggaagagt cgcttaaggc cctgccgtgg gacagtttgc tggaaaatcc
     1441 gcagttagtg ttattgcagg cgtggctaat gcaaagccaa catcgctatg gcgaagttaa
     1501 caccctgcta gcccgtgctg aacatgaaat caaggacatc agagaaggca ccatgcacgc
     1561 agaatttaac gctctgcgcg cccaggtggc gattaacgat ggtaatccgg atgaagcgga
     1621 acggctggca aaactggcac tggaagagct gccgccgggc tggttctata gccgcattgt
     1681 ggcaacctcg gtgctgggtg aagtgctgca ctgcaaaggc gaattgaccc gctcactggc
     1741 gctagtgcag caaaccgaac agatagcacg ccagcacgac gtctggcact acgccttatg
     1801 gagtttaatt cagcaaagtg aaattctgtt tgcccaaggg ttcctgcaaa ccgcgtggga
     1861 aacgcaggaa aaagcgttcc agctgatcaa cgagcagcat ctggaacagc tgccaatgca
     1921 tgagtttctg gtgcgcatcc gagcacaact gttatgggcc tgggcacggc tggatgaagc
     1981 cgaagcgtcg gcgcgtagcg ggattgaagt cttgtcgtct tatcagccac agcaacagct
     2041 tcagtgcctg gcaatgttga ttcaatgctc gctggcccgt ggtgatttag ataacgcccg
     2101 tagccagttg aaccgtctgg aaaacctgct ggggaatggc aaatatcaca gcgactggat
     2161 ctctaacgcc aacaaagtcc gggtgattta ctggcaaatg accggcgata aagccgccgc
     2221 tgccaactgg ttgcgtcata cggctaaacc ggagtttgcg aacaaccact tcctgcaagg
     2281 tcaatggcgc aacattgccc gcgcacaaat cttgctgggc gagtttgaac ctgcggaaat
     2341 tgttctcgaa gaactcaatg aaaatgcccg cagtctgcgg ttgatgagcg atctcaaccg
     2401 taacctgttg ctgcttaatc aactgtactg gcaggccgga cgtaaaagtg acgcccagcg
     2461 cgtgttgctg gacgcattaa aactggcgaa tcgcaccgga tttatcagtc attttgtcat
     2521 cgaaggcgaa gcgatggcgc aacaactgcg ccagctgatt caacttaata cgctgccgga
     2581 actggaacag catcgcgcgc agcgtattct gcgagaaatc aatcaacatc atcggcataa
     2641 attcgcccat ttcgatgaga atttcgttga acgtctgcta aatcatccgg aagtgccaga
     2701 actaatccgc accagcccgt tgacccaacg tgaatggcag gtcctggggc tgatctactc
     2761 cggttacagc aatgaacaaa ttgcgggcga actggaagtg gcggcaacca ccatcaagac
     2821 gcatatccgc aatctgtacc aaaaactcgg cgtcgcccac cgtcaggctg ccgtgcagca
     2881 cgcgcagaaa ctgctgaaga tgatgggtta tggggtgtga gtttagccgg ataatgcatt
     2941 ggatccggtt tacatcttta catcattcaa tactcacccg cgttacgcca tctgtttcta
     3001 tcaaaccaaa ccgcaccggc aagaaacgct ccaccaccgc gatattggtc agcagatggc
     3061 atgaaggatg ggcgaccgta aattctcccg cgcccgccag cgccatcggc agtaccagtt
     3121 ggtcggcgag atattccccc accgccgctg ggcttgccag gtagcgtttc acctctttca
     3181 ctaactgcgc ggctaccacc tcggcactga cgcgcttttc accgacgacg aaaaagcgtt
     3241 cagtgatatt ttcactttcg acttccagcg agacggtatt acccggcccc tggtcgcgcg
     3301 gcaggttatg aatattctgt tcatgcaggg aaaaaatccc cgccagtgta gcgatttcac
     3361 gctcagcaac atggcgcggc acaccagcta atagaacttc tccacgcatc tgcacaatgt
     3421 tcccgcgctc gccaagttgc aaggtgctaa acgatgccac gggcgagact tccgttgcca
     3481 ccacaccgcc tccggcagga taaaaaccgt gacgtaacag cgtggtttgc tgatgaattc
     3541 ctattttcgc cagcagcggc tccagcaccc ggcggataaa atccgcaggc ggcgccgacg
     3601 ggttatcggt gccgccgctc acttcaacac gcgaaggtcc atcggcaaac cacagcgcgg
     3661 gcagcaccgt ttgcagcacc agcgtacaac ttccggcgct accgatagca aagcggtaat
     3721 cgccgccgcg cacggtgcca ggccggaaga ccagacgctg cgatcccagc tcagcgccga
     3781 gcgcaggatc tgcccgccgc cttcgccctg tgcgccatcc agcgcaatca tccttttcat
     3841 catttatcct tttacgcaca ccacctgacg cagggtgtag ataacttcca ccagatcgct
     3901 ttgtgccgcc atcaccgcat caatatcttt atacgccatc gggatttcgt cgatcacttc
     3961 ggcatcttta cggcattcca catgcgcggt ggcacgaatt tggtcttcca cgctgaacag
     4021 ttttttcgct ttagttcggc tcattacccg cccggcaccg tggctgcacg aacagaacga
     4081 ctcttcattt cccagcccac ggacgataaa gctttttgct cccatcgaac cgggaataat
     4141 tccatattga ccagcacgcg cagacaccgc acctttacgc gtcacgtaga tctcttcacc
     4201 aaagtgctgt tctttttgca catagttgtg gtgacagttg atctcttcca tcgccagcgt
     4261 ttgtggctgt ctgaccgttt tctgcgtaat gctctgcaac gccgttacca cattttccat
     4321 catcgcatcg cggttaaggc tggcgaaaag ctgcgcccag gccacggctc ttaggtaatc
     4381 atcaaagtat tccgtacctt ccataaagta cgccagatca cgcgacggca acgtctcaag
     4441 ttggtcctgc atctcttttt gcgccagatc gataaagtaa gtcccgatgg cgttaccaat
     4501 tccacgtgaa ccggagtgca gcataatcca cacctgctcc gactcatcaa ggcagatttc
     4561 aataaagtgg ttacccgttc ccagcgttcc caggtgttta tagttgttag tgttaaggaa
     4621 acgcggatat ttttgcgtta accactgata accggcctcc agctcagccc atttcgcatc
     4681 gacgttaaaa ggcgggtttt cccatgcgcc tttatcacgt ttacaacgcc cggtagtacg
     4741 cccgtgtggt acggccgttt caatcgcttg acgcagctcc gccaggtttt caggcaggtc
     4801 tgccgccgtt aacgcggtac gcagcgcgtt cattccacag ccaatatcca cgcccaccgc
     4861 cgccggaata atcgcccctt tggtcgggat cacgctacca atggtggaac ctttacccag
     4921 gtgtacatca ggcattaccg caatatgttt gaaaataaac ggcatcttcg ccgtattaat
     4981 aagttgctga cgcgcatcgg cttctaccgg cacgcctttg gtccacattt ttaccggggc
     5041 attttcagtg gtcagtaatt cgtaattcat tttgtttttc tcttttcgtt gtttgcggtc
     5101 ctgataattg caaccgtcgt gccagaaaat taaataagca gattaatttt ttaatttatt
     5161 gtttctaaaa agattatatc tttatgtccg taaacggaga tttcccgcaa agccgattta
     5221 ccgataatga aatatcgcct tttataagga tatctaagat gcgtaaaaca gtggcttttg
     5281 gctttgtcgg taccgtgctg gattatgccg gacgcggcag tcagcgctgg tcaaaatggc
     5341 gtccgtcact ctgtatatgc cagcaagaat cgttggtcat cgatcgactg gaattattgc
     5401 acgatgcccg ctcgcgctcg ctatttgaaa cgcttaaacg cgatatcgcc agcgtttcgc
     5461 cagaaacaga agtggtgggc gttgagattg aactgcataa cccgtgggat ttcgaagagg
     5521 tttacgcctg cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa aaagaagact
     5581 atttaattca catcaccacc ggcacccacg tcgcgcagat ttgctggttt ctgctggcgg
     5641 aagcgcgtta tctacccgcc cggttgatac aatcttcaac tccgcgcaaa aaagaacagc
     5701 cgcgcggccc aggtgaagtg acgattatcg atctcgattt aagccgttat aacgccatcg
     5761 ccagccgctt tgccgaggaa cggcagcaaa cgctcgattt cctcaaatcc ggtattgcca
     5821 cgcgtaaccc ccacttcaac cgcatgattg agcagatcga aaaagtggcg attaaatccc
     5881 gcgcgccgat tctgcttaac ggcccgaccg gcgcaggtaa gtcatttctg gcgcgacgca
     5941 tcttcgagtt aaaacaggca cggcatcagt ttagcggcgc gtttgtggaa gtgaactgcg
     6001 ccaccctgcg cggcgatacc gcgatgtcga cgctgtttgg tcatgtgaaa ggcgcgttta
     6061 ccggggcgca ggaatctcgt gaaggattat tacgcagcgc caacggcgga atgttgtttc
     6121 ttgatgagat tggcgaactg ggcgcagacg aacaggcaat gctgctgaaa gctattgaag
     6181 agaaaacctt ttacccgttt ggcagcgatc gccaggtgag cagtgatttt cagcttatcg
     6241 ccggaacggt gcgcgatttg cgccagctgg ttgccgaagg caaattccgc gaagatctgt
     6301 acgcgcggat caatctctgg accttcaccc tgccgggtct acgccagcgc caggaagata
     6361 ttgaaccgaa cctggattat gaagtggagc gccacgccac actcactggt gacagcgtgc
     6421 gttttaacac cgaagcgcgg cgtgcctggc tggcctttgc aacatctccc caggctacat
     6481 ggcgcggtaa ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc tttgccacta
     6541 gcggacgcat cactctggac gtggtggaag atgagataaa ccgtctgcgc tataactggc
     6601 aggagagccg cccctccgcg cttacggcgt tgctgggcgc agaggcagaa aacatcgatc
     6661 tcttcgaccg tatgcaactg gaacacgtta tcgctatctg ccgccaggca aagtcgcttt
     6721 ctgctgccgg acgacagctt tttgatgttt cgcgccaggg caaagccagc gtcaatgacg
     6781 cggatcggct acgcaaatac ctggcgcgtt ttggtctgac gtgggaggcc gtgcaggatc
     6841 agcacagctc cagttgaata tggtggtccg tcagcacctg catcacgctt actggcggcg
     6901 gggcgtcggt gtagacggca tcaaccatgc tgatgctccc catattgacc atcgcattac
     6961 ggccaaattt tgaatggtca accaccagca taacgtggcg cgagttctca ataatggcgc
     7021 gcttggtacg aacttcgtgg taatcgaact ccagcagcga gccgtcgctg tcgatgccgc
     7081 tgatccccag aatgccgaaa tcaaggcgga actgggagat aaaatcgagc gtcgcttcgc
     7141 caatgatccc gccatcgcgg ctacgtaatt cgccaccggc gagaataatg cggaaatctt
     7201 cttttaccat caacgtgtta gcaacgttga gattgttggt gacaatgcgc aaattgctgt
     7261 gattgagcag cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata aacagcgtcg
     7321 agccattggg gatttgctcc gccactttgc gggcgatgcg ctctttttct tcggtctggg
     7381 tggctttacg atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc gcgccgccat
     7441 gatggcgcag gatcaggttt tgctctgcca gctcgttgag gtcgcggcga atagtctgcg
     7501 ggctgacgga gaagtgctcc accagttcct cggtactgac ataaccctgc tgtttaacca
     7561 gttcgataat accgttgtga cgttgtgttt gtttcattta taaatccctg gaattatttt
     7621 cgttttcgcg cattgagcga atcaacaaaa gccatcgcta aacccacggc taatccggcg
     7681 atgtgtgctc cgttcgccat cgacatccca aacaaatcaa accatccggc gacaatccag
     7741 atcagcgcaa agataattaa cccacgttgc aggtaaatgc cactttgcgg atcgcgttcg
     7801 ccacgtagcc agacgtagcc catcagcgca tacaccacgc cagaaagccc gccaaaccac
     7861 ggcccgctga atttttgctg cacatagccg cttaacaggg cgctgataag cgtaatgaca
     7921 attagcttac cgctaccgag gcgtttttcc accgcgccgc cgagatacca ccaccaaagc
     7981 aggttaaaga ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa gtaacgccag
     8041 aactcaaatt tcagtgttgg atcgaatggc caggccagcc ataacatcac ttcctgatcg
     8101 ccgagaattt gcatggcaat aaacaccacc acgcaggcga tcatcatcac ccaggtcacc
     8161 ggacctgcgc gttcacgcaa ggcagcaaag aaaggataac ggcgataatg caggccactg
     8221 ccggtatggc ctgccagcca gctggccgcc agataacgcg gatctgccgg gttttcgaga
     8281 aaacgcgcca gctccgcccg cacgcgctcg gcctgggact catccgccag ccagacatcg
     8341 ctttggttat gttgttgaat cgtgaggata acaccctgcg tcgccatgta atcaacaaac
     8401 gcctgcgcca cgcgggggtt agcaaaagag gtaatcatca acatcgttgc tgtcgcttat
     8461 tccacacaaa aggggacagt ataaagcgtt acgcgccgta cgccacctct gcgggaaact
     8521 gtcgctgcca ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag ccctgttgca
     8581 gcagatactg cgccgcccct ttgctgctat tgccgtgata acacatcacc atcaccggag
     8641 tgtcaaagtc gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa tggaaagcct
     8701 gcaccgcatg ccccatagcg aaactctgtg gatcgcgaat atcgaccagc accgcctctt
     8761 tttcctgcaa cttctggtgc gcgtcggcaa cgttaataca ttcgaactga tccatgcgtc
     8821 tctctttctt tacaaacaag tgggcaaatt taccgcacag tttacgtcga agcggcagat
     8881 aaacgccata atgttataca tatcactcta aaatgttttt tcaatgttac ccaaagcgcg
     8941 attctttgct aatatgttcg ataacgaaca tttatgagct ttaacgaaag tgaatgaggg
     9001 cagcatggaa accaaagatc tgattgtgat aggtggcggc atcaatggtg ctggtatcgc
     9061 ggcagacgcc gctggacgcg gtttatccgt gctgatgctg gaggcgcagg atctcgcttg
     9121 cgcgacctct tccgccagtt caaaactcat tcacggtggc ctgcgctacc ttgagcacta
     9181 tgaattccgc ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga aaatggctcc
     9241 gcatatcgcc ttcccgatgc gttttcgcct gccacatcgt ccgcatctgc gcccggcgtg
     9301 gatgattcgc attggtctgt ttatgtacga tcatctgggt aaacgcacca gcctgccggg
     9361 atcaattggt ttgcgttttg gcgcaaattc agtgttaaaa ccggaaatta agcgcggatt
     9421 cgaatattct gactgttggg tagacgacgc ccgtctggta ctcgccaacg cccagatggt
     9481 ggtgcgtaaa ggcggcgaag tgcttactcg gactcgcgcc acctctgctc gccgcgaaaa
     9541 cggcctgtgg attgtggaag cggaagatat cgataccggc aaaaaatata gctggcaagc
     9601 gcgcggcctg gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg aggggatgca
     9661 tctgccttcg ccttatggca ttcgcctgat caaaggcagc catattgtgg tgccgcgcgt
     9721 gcatacccaa aagcaggcct acattctgca aaacgaagat aaacgcattg tgttcgtgat
     9781 cccgtggatg gacgagtttt ccatcatcgg caccaccgat gtggaataca aaggtgatcc
     9841 gaaagcagtg aagattgaag agagtgaaat caattacctg ctgaaagtgt ataacgcgca
     9901 ctttaaaaag cagctaagcc gtgacgatat cgtctggacc tactccggtg tgcgtccgct
     9961 gtgtgatgat gagtccgact cgccgcaagc cattacccgt gattacaccc ttgatatcca
    10021 tgatgaaaac ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga ccacctaccg
    10081 aaaactggca gaacatgcgc tggaaaaact gacgccgtat tatcagggta ttggcccggc
    10141 gtggacgaaa gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg acgattacgc
    10201 cgctcgcctg cgccgccgtt acccgttcct gactgaatcg ctggcacgtc actacgcgcg
    10261 cacttacggc agcaacagcg agctgctgct cggcaatgcg ggaacggtaa gcgatctcgg
    10321 ggaagatttc ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg atcacgaatg
    10381 ggtccgccgc accgacgatg ccctgtggcg tcgcacaaaa caaggcatgt ggctgaatgc
    10441 ggatcaacaa tctcgtgtga gtcagtggct ggtggaatat acgcagcaga agttgtcgct
    10501 ggcgtcgtaa attaacgtaa ggtgatcagg tcagatttca atctggcctg agactgatga
    10561 caaacacaaa actgcctgat gcgctacgct tatcaggcct acgtggttta tgcaatatat
    10621 tgaatttgca tggtcttgta ggccagataa gacgttcacg tcgcatccgg catgaacaaa
    10681 gcgcactttg tcaaaaatct aacctacttt ttaattcagg gaattaccgc aaagcccacg
    10741 ccatcatacc caacgtaaca aaactcaggc acgttcccca ctcgccccga gaaaaatagc
    10801 attaatgcgc ccagcgtcag cataaaaata ttgagcagaa ggtaaattgt tggcgtaaag
    10861 agtaaattgc aaacctcagg aatagcggca agcccagccc ccagcatggc aataaccgct
    10921 gcaaaaaatt gcgtcagtat tgggatgccg aaaaaagtca ttaccagcga ggtcaaaatc
    10981 cattttctat tttgcattat tctttccatt ctttctgaat ggcgaaatta tactcccctt
    11041 gccccttctg gacacttttc cgaaatgatg gcgaaaaaaa cgggaccctt tgg
//
LOCUS       DADMEQ010000125        10818 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.126, whole genome shotgun sequence.
ACCESSION   DADMEQ010000125 DADMEQ010000000
VERSION     DADMEQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.126"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            90..1175
                     /locus_tag="JNZ05_003341"
                     /pseudo
     CDS             90..1175
                     /locus_tag="JNZ05_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            1252..1860
                     /locus_tag="JNZ05_003342"
     CDS             1252..1860
                     /locus_tag="JNZ05_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAY8566455.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1958..3349
                     /gene="selA"
                     /locus_tag="JNZ05_003343"
     CDS             1958..3349
                     /gene="selA"
                     /locus_tag="JNZ05_003343"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HAY8566456.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3346..5190
                     /gene="selB"
                     /locus_tag="JNZ05_003344"
     CDS             3346..5190
                     /gene="selB"
                     /locus_tag="JNZ05_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAY8566457.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPER"
     gene            5380..6531
                     /gene="yiaY"
                     /locus_tag="JNZ05_003345"
     CDS             5380..6531
                     /gene="yiaY"
                     /locus_tag="JNZ05_003345"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HAY8566458.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            6696..8234
                     /locus_tag="JNZ05_003346"
     CDS             6696..8234
                     /locus_tag="JNZ05_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY8566459.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRCIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(8453..8608)
                     /locus_tag="JNZ05_003347"
     CDS             complement(8453..8608)
                     /locus_tag="JNZ05_003347"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566460.1"
                     /translation="MAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRP
                     LTIETRD"
     gene            8779..9102
                     /locus_tag="JNZ05_003348"
     CDS             8779..9102
                     /locus_tag="JNZ05_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY8566461.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLISKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            9108..10244
                     /locus_tag="JNZ05_003349"
     CDS             9108..10244
                     /locus_tag="JNZ05_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY8566462.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQVR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(10241..>10818)
                     /locus_tag="JNZ05_003350"
     CDS             complement(10241..>10818)
                     /locus_tag="JNZ05_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8566463.1"
                     /translation="ERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYAS
                     QRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNT
                     QSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRV
                     QYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         2577 a         2658 c         2978 g         2605 t
ORIGIN      
        1 gccgcacttt ctgaccgaat ttcccggctg gagcatcaac tcgccgccga gaaaaagact
       61 gactattcca cgttcccgga gatctaatta tggatctatt gattgtttta acttacgtgg
      121 cgctggcgac ggcggcgctg ggaggcgtgt ttctggtgag tggtttgatt ttgttgatga
      181 actacaacca cccttacact tttaccgcgc aaaaggcagt gatagcgatc cctatcacgc
      241 cacaggtgac gggaattgtt actgaagtca ctgacaagaa taatcagctt attcaaaagg
      301 gcgaggtgct ttttaagctc gacccggttc gttaccaggc gcgagttgac agacttcagg
      361 ctgacctgat gacggcgacg cataatataa agacgctgcg tgcgcagctc actgaagcgc
      421 aggccaacac cacccaggtt tcagcggagc gcgaccgtct gtttaaaaat tatcaacgtt
      481 acctgaaagg cagccaggcg gcggtgaatc cgttctcgga acgtgacatc gacgatgcgc
      541 ggcaaaattt cctcgcgcag gatgcgttag tgaaaggctc ggtggcggag caggcgcaga
      601 tccagagtca gctcgacagt atggtcaacg gcgagcaatc gcagattgtg agcttaagag
      661 cgcaacttac tgaagcaaaa tataatcttg agcagactgt cattcgcgca ccaagcaatg
      721 gctacgtcac ttaggtactg atccgcccag gtacatacgc agctgccttg ccgctgcgtc
      781 cggtgatggt cttcatcccc gagcaaaaac ggcaaattgt cgcccaattt cggcaaaact
      841 cgctgttacg tctgaaacct ggcgatgatg cggaagtggt gtttaacgcg ctacctgggc
      901 aggtgttcca cggcaaattg actagtattt tacctgtcgt gccaggcggt tcttatcagg
      961 cgcagggggt attgcaatca ttaacggtcg ttcccggcac ggacggtgtg ctgggaacca
     1021 ttgaactgga ccctaacgat gatatcgatg ccttacccga cggcatctac gcccaggtgg
     1081 cggtttactc cgaccatttc agccatgttt cggtgatgcg gaaagtgctg ctaagaatga
     1141 ccagttggat gcattatctt tatttggatc attgagactt atatcacctg tgttttgcgt
     1201 gagcctgagc ctgagccata ctgatatttt tcctcgcaca aaggactcgg catgaaactc
     1261 gtcggtagct acaccagccc gtttgtacgc aaactttcta ttctgttgtt agaaaagggc
     1321 ataactttcg aatttattaa tgaactgccc tataacgcgg ataacggcgt ggcgcaattt
     1381 aacccgttag ggaaagtgcc ggtgctggtg accgaagagg gcgaatgctg gtttgattcg
     1441 ccgatcatcg ctgaatatat tgagttaatg aatgtcgctc cggcgatgtt gccgcgcgat
     1501 ccgctggagt cgttgcgggt gcgcaaaatt gaggcgctgg cggatggcat tatggatgcc
     1561 gggctggtat cggtgcgtga acaggcgcgt ccggcggcgc agcagtctga agatgaattg
     1621 ttacgccagc gggagaaaat caaccgcagt ctggatgtgc tggaaggata tctggtcgat
     1681 ggcacactca aaaccgatac ggtcaatctg gcgactatcg ccattgcctg tgctgtcgga
     1741 tatctcaatt tccgccgcgt tgcgccaggt tggtgtgtcg accgcccaca cttagtcaaa
     1801 ctggtcgaaa acctgtttag ccgcgaaagt tttgctcgca ccgaaccgcc aaaggcttga
     1861 tgcgcgatat gtcctcctga cccatctcac gttacaatcc gtggttatgt taaacgccct
     1921 tctccgtgtg agagggcctt gatcagccag gtttcctatg acaaccgaaa cgcgttccct
     1981 ctatagtcaa cttccggcta ttgatcgctt attacgcgat agctccttcc tttctttgcg
     2041 tgatacttat ggtcacaccc gcgtggtgga attgttgcgt cagatgctcg acgaagcgcg
     2101 agaagtgatt cgtggcagcc agacgctgcc tgcgtggtgt gaaaactggg cgcaagaagt
     2161 cgatgcccgg ttgacgaaag aggcgcagag cgcgctgcgt ccggtgatca acctgacggg
     2221 aaccgtgctg cataccaacc ttgggcgagc tttacaggcg gaagccgcgg tggaagccgt
     2281 tgcgcaggct atgcgttcgc cagtgaccct cgagtacgat ctggacgacg ccggacgcgg
     2341 acatcgcgat cgggcgctgg cgcagctgct gtgccgtatt acgggggcgg aagatgcctg
     2401 tatcgtcaat aacaatgcgg cggcggtgtt attgatgttg gcggccactg ccagcgggaa
     2461 agaggtggtg gtatctcgcg gcgaactggt ggagattggc ggcgcgtttc gtattcccga
     2521 tgttatgcgt caggcaggct gcaccctaca cgaagtaggg accaccaacc gcacgcacgc
     2581 gaatgattat cgtcaggcgg tgaatgaaaa taccgcactg ttgatgaaag tacataccag
     2641 taactacagc attcaggggt tcaccaaagc gatagacgaa gctgaactgg tggcgctcgg
     2701 caaagagctg gatgttccag tggtgactga tttaggcagt ggctcgctgg tcgatcttag
     2761 ccagtacggt ttgccgaaag agccaatgcc gcaggagttg attgcggcgg gcgtcagtct
     2821 ggtgagcttc tccggcgaca agttgttagg cgggccgcag gcagggatta tcgttggtaa
     2881 aaaagagatg atcgcccgcc tgcaaagcca cccgctgaag cgtgcattac gcgcggataa
     2941 aatgaccctc gcggcgctgg aagccacgtt gcgtctttat ttacaccctg aagctctgag
     3001 tgaaaaatta ccgaccctgc gcctgcttac ccgcagcgca gaggtcattc aaatccaggc
     3061 acaacgttta caggctcccc ttgtcgcaca ttacggcgcg gagtttgcgg tacaggttat
     3121 gccatgtctt tcgcagattg gcagtggttc gctgccggtt gatcgcctgc cgagcgcggc
     3181 attaacgttt acaccccatg atggacgcgg tagccacctt gagtcattag ccgcccgctg
     3241 gcgtgaattg ccagtgccgg tgattggtcg tatttatgac ggacgattgt ggctggattt
     3301 acgctgcctt gaagatgagc aacggttttt ggagatgttg ttgaaatgat tattgcgact
     3361 gccggacacg ttgaccacgg caaaacaacc ttattgcagg cgattactgg cgtaaatgct
     3421 gaccgtctgc cggaagaaaa aaagcgcggc atgaccatcg atctcggcta tgcctactgg
     3481 ccgcagccgg atggtcgcgt gcctggtttt atcgacgttc ccggtcatga aaagtttctt
     3541 tccaacatgc tggcgggcgt tggtggtatc gatcacgcgc tgttggtggt ggcgtgcgat
     3601 gacggcgtga tggcacagac ccgtgagcat ctggcgattt tgcagctgac cggtaacccg
     3661 atgctgacag tggcgctgac caaagccgat cgtgtggacg aagcgcgtgt tgatgaggtt
     3721 gaacgccagg taaaggaggt tctgcgggaa tacggttttg ctgaggcaaa actgtttatc
     3781 accgcagcaa ccgaaggtcg gggaattgat gccctgcgcg agcatctgct tcagttgccg
     3841 gaacgcgagc acgccagcca acatagtttc cgcctcgcga ttgaccgcgc atttaccgta
     3901 aaaggtgccg ggctggtcgt caccggtacg gcgttaagcg gggaagtgaa ggtaggcgat
     3961 tcactctggc tgactggtgt aaataaaccg atgcgtgtac gtgcgctgca tgcgcaaaac
     4021 cagccaacag aaaccgccaa tgccgggcag cgtatcgcgc ttgacatcgc gggtgatgcg
     4081 gaaaaagagc agattaaccg tggcgactgg ctgcttgccg atgtgccgcc agagccgttc
     4141 acacgggtga ttgtcgagct tcaaacccat gcaccgctga cccagtggca gccgctgcat
     4201 attcaccacg ccgccagcca cgtcacggga cgtgtttcac tgctggaaga taaccttgct
     4261 gaactggtct tcgacacccc gttatggctg gcagataacg accgcctggt attgcgcgat
     4321 atctctgccc gcaacacgct ggccggagcg cgcgtcgtga tgcttaaccc gccgcgtcgc
     4381 ggtaaacgta aaccagagta tctgcaatgg ctggcttccc tcgccagggc acagaatgat
     4441 agcgaagcgt tagccgttca cctgcaacgc ggcgcggtta atcttcctga tttcgcctgg
     4501 gcgcgccagc tcaacggcga agggatgcgc gaactgctgc aacagcctgg ctatattcag
     4561 gctggttata gcttgttgaa tgcgccggtt gccgctcgct ggcaacggaa aattctcgac
     4621 acattagcga cttatcatga gcaacatcgc gatgaacctg ggcctgggcg cgaacgtctg
     4681 cgacgtatgg cgttgccaat ggaagatgaa gcgctagtac tgttgctgat tgaaaagatg
     4741 cgcgaaagcg gcgacatcct cagtcatcac ggttggctgc atctgccgga tcacaaagcg
     4801 ggcttcagcg aagagcagca ggccatctgg caaaaagcag agccgctgtt tggtgacgag
     4861 ccgtggtggg tgcgtgatct ggcaaaagag acgggtaccg acgagcaggc aatgcgcctg
     4921 acgctacgcc aggcggcgca gcaaggaata attaccgcga tcgtcaaaga tcgttattac
     4981 cgtaacgatc ggattgtcga atttgccaat atgatccgcg atctcgatca ggagtgtggt
     5041 tcaacctgcg cggcggattt ccgcgatcgc ttaggcgtag gccgaaagct ggcaattcag
     5101 attctggaat attttgaccg cattggcttt acgcgtcgtc gtggaaatga tcatttattg
     5161 cgcgacgcat tattatttcc ggaaagataa ggaaatgatt aattaatttt taaaataaat
     5221 taatacaaat ttcttatgat tttaaataaa gcacattgtt taatgaatac aatgtgcttt
     5281 ttattagatt aattttgata attaataata atgatcatgt tttcgaatga aaatcctcag
     5341 taagctgccc accctttttt acactttcag gagtgtgtta tggcatcttc aactttcttt
     5401 attccttctg tgaatgtcat cggcgctgat tcattgactg atgcaatgaa tatgatggca
     5461 gattatggat ttacccgtac cttaattgtc actgacaata tgttaacgaa attaggtatg
     5521 gcgggtgatg tgcaaaaagc actggaagaa cgcaatattt ttagcgttat ttatgatggc
     5581 acccaaccta accccaccac ggaaaacgtc gccgcaggtt tgaaattact taaagaaaat
     5641 aattgcgata gcgtgatctc cttaggcggt ggttctccac acgactgcgc aaaaggtatt
     5701 gcgctggtgg cagccaatgg cggcgatatt cgcgattacg aaggcgttga ccgctctgca
     5761 aaaccgcagc tgccgatgat cgccatcaat accacggcgg gtacggcctc tgaaatgacc
     5821 cgtttctgca tcatcactga cgaagcgcgt cataacaaaa tggcgattgt tgataagcac
     5881 gtgactccgc tgctttctgt caatgactcc tctctgatga ttggtatgcc gaagtcactg
     5941 accgccgcaa cgggtatgga tgccttaacg cacgctatcg aagcgtatgt ttctattgcc
     6001 gccacgccga tcactgacgc ttgtgcactg aaagccgtga ccatgattgc cgaaaacctg
     6061 ccgttagccg ttgaagatgg cagtaatgcg aaagcgcgtg aagcaatggc ttatgcccag
     6121 ttccttgccg gtatggcgtt caataatgct tctctgggtt atgttcatgc gatggcgcac
     6181 cagctgggcg gtttctacaa cctgccacac ggtgtatgta acgccgttct gctgccgcat
     6241 gttcaggtat tcaacagcaa agtcgccgct gcacgtctgc gtgactgtgc cgctgcaatg
     6301 ggcgtgaacg tgacaggtaa aaacgacgcg gaaggtgctg aagcctgcat taacgccatc
     6361 cgtgaactgg cgaagaaagt ggatatcccg gcaggcctac gcgacctgaa cgtgaaagaa
     6421 gaagatttcg cggttctggc gactaatgcc ctgaaagatg cctgtggctt tactaatccg
     6481 atccaggcaa ctcacgaaga aattgtggtg atttatcgcg ccgcaatgta atcatcattc
     6541 caacaacggc tggcaaattg tcagccgctt tttcaactat ctctgtaatc cttgcccgta
     6601 aattcgtgat agctgtcgta aagctgttac cgactggcga agatttcgcc agtcacgtct
     6661 acccttgtta tacctcacac cgcaaggaga cgatcatgac caataatccc ccttcagcac
     6721 agattaagcc cggcgagtat ggtttcccca tcaagttaaa agcccgctac gacaacttta
     6781 ttggcggtga atgggtagcc cctgccgatg gtgagtatta ccagaacctg acgccggtga
     6841 ccgggcagct gctgtgcgaa gtggcgtctt cgggcaaacg agacatcgat ctggcgctgg
     6901 atgctgcgca caaagtgaaa gataaatggg cgcacacctc ggtgcaggat cgcgcggcga
     6961 ttctgtttaa gattgccgat cgaatggaac aaaacctcga gctgttagcg acagctgaaa
     7021 cctgggataa cggcaaaccc attcgcgaaa ccagtgctgc ggatgtaccg ctggcgattg
     7081 accatttccg ctatttcgcc tcgtgtattc gggcgcagga aggtgggatc agtgaagttg
     7141 atagcgaaac cgtggcctat catttccatg aaccgttagg cgtggtgggg cagattatcc
     7201 cgtggaactt cccgctgctg atggcgagct ggaaaatggc tcccgcgctg gcggcgggca
     7261 actgtgtggt gctgaaaccc gcacgtctta ccccgctttc tgtactgctg ctaatggaaa
     7321 ttgtcggtga tttactgccg ccgggcgtgg tgaacgtggt caatggcgca ggtggggaaa
     7381 ttggcgaata tctggcgacc tcgaaacgca tcgccaaagt ggcgtttacc ggctcaacgg
     7441 aagtgggcca acaaattatg caatacgcaa cgcaaaacat tattccggtg acgctggagt
     7501 tgggcggtaa gtcgccaaat atcttctttg ctgatgtgat ggatgaagaa gatgcctttt
     7561 tcgataaagc gctggaaggc tttgcactgt ttgcctttaa ccagggcgaa gtttgcacct
     7621 gtccgagtcg tgccttagtg caggagtcaa tctacgaacg ctttatggaa cgcgccatcc
     7681 gccgtgtcga aagcattcgc agcggtaacc cgctcgacag cgtgacgcaa atgggcgcgc
     7741 aggtttctca cgggcaactg gaaaccatcc tcaactacat tgatatcggt aaaaaagagg
     7801 gcgctgacgt gctcacaggc gggcggcgca agctgctgga aggtgaactg aaagacggct
     7861 actacctcga accgacgatt ctgtttggtc agaacaatat gcgggtgttc caggaggaga
     7921 tttttggccc ggtgctggcg gtgaccacct tcaaaacgat ggaagaagcg ctggagctgg
     7981 cgaacgatac gcaatatggc ctgggcgcgg gcgtctggag ccgcaacggt aatctggcct
     8041 ataagatggg gcgctgcata caggctgggc gcgtgtggac caactgttat cacgcttacc
     8101 cggcacatgc tgcgtttggt ggctacaaac aatcaggtat cggtcgcgaa acccacaaga
     8161 tgatgctgga gcattaccag caaaccaagt gcctgctggt gagctactcg gataaaccgt
     8221 tggggctgtt ctgatataag aagctggtcg cattgggtat tcattgcctg atgcgacgct
     8281 tacgcgtctt atcatgccta cgggaacctg accgtaggcc ggataaggcg tacacgccgc
     8341 atccggcata aacaaagcgc acgttgttac aatttacgtt ttaaacctac gtgcgcaata
     8401 acccagacta cgccaacaga agtgcgtttc tggggcgtgt tttgtcagga gtttaatctc
     8461 gcgtttcgat ggtgagcggg cgaaccaggc cgtaaatcac cattggtgat ccgaggccaa
     8521 tcagcaataa acggtagata attgaaggtt taatacgctt aatctgtact gtggatgttt
     8581 tactggagtt attcattagt ttggccaata gttccactat ttcaatatga taaacctcgt
     8641 cctgttgaaa aaccagacag cacatttcag aaagtatata aaaaacaata tgtcgttata
     8701 taaataagga aattgcactg catcatggca tttcataata tgcgttaatt gttctttttt
     8761 atattctttg agggaaatat gttcctggac tattttgcac tgggagtgct tatttttgta
     8821 tttctggtga tattctatgg gattattatt ttacatgata ttccctacct gatttctaaa
     8881 aaacgtaatc atcctcatgc cgacgccatt catgttgctg gttgggtgag tctttttacg
     8941 ctgcatgtta tctggccatt tttgtggatt tgggccacgc tttaccgccc ggagcgggga
     9001 tggggaatgc aaatccatga ctcctccgtt gtgcaactac aacagcgtat tgccgggctg
     9061 gagaaacagc tcgccgacat caaatcctct tctgccgagt aatacttatg gatttactga
     9121 ttattttgac ctatgtggct tttgcatggg caatgtttaa gatcttcaaa attcctgtaa
     9181 ataaatggac catttccaca gcggccctgg gtggcatatt tattgtcagt ggtttaattc
     9241 tgttaatgaa ctataaccat ccgtatacct tcaaggcgca aaaagcggtt atttctattc
     9301 ctgttgtccc acaggtgaca ggcgtggtga tcgaagtgac ggataagaaa aatacgctga
     9361 ttaaaaaagg tgaggtgcta tttcgactgg acccgacgcg ttatcaggtg cgggtggatc
     9421 ggctgatggc ggatatcgtt accgcagaac ataaacagcg ggcgttgggc gcagagttag
     9481 atgagatggc ggcgaatact cagcaggcaa aggccacgcg ggataaattc gctaaagagt
     9541 atcagcgtta cgcacgcggc agccaggcga aagtaaaccc gttttcagaa cgcgatattg
     9601 atgtggcgcg gcaaaattat ctggcgcagg aagcctccgt aaagtcatcg gcggcggaac
     9661 aaaaacagat ccagagccag ctggatagcc tggtgttggg tgaacattct caaatcgcca
     9721 gcctgaaagc gcagctcgcg gaagcaaaat ataaccttga gcagacgata gtgcgtgcgc
     9781 cgagcgatgg ttatgtgacc caggtgctga ttcgtccggg tacctatgcc gcgtcgctgc
     9841 cgctacgtcc ggtgatggtg tttatacccg atcagaaacg acaaattgtg gcgcagttcc
     9901 gtcagaactc cttgctgcga ctggcccctg gcgacgatgc ggaagtggtg tttaatgctc
     9961 tgccaggtaa ggtattcagc ggcaagctgg cagccattag tccagctgtt cccggcggag
    10021 cttatcagtc gaccggcacc ttacagacgt taaacacagc gccgggttca gatggcgtta
    10081 tcgcgaccat tgaactggat gagcacactg atttgagcgc tttaccagac ggtatttacg
    10141 cccaggtggc ggtctactct gatcatttca gccatgtctc ggtgatgcgc aaagtgctat
    10201 tacgcatgac cagctgggtg cattaccttt atctcgatca ttaatcttcg ctttccgccg
    10261 cttgttcagg ccgcagttgc tgcgactgag caagctggtg gcgaatttca cgcagcattt
    10321 catccagcac atactggacc cgccagggca ggtattcccg tttacggaag atagccacca
    10381 gatccagcca ccattcatac gggtgctgaa agcagggcac cagcgtgccg tcttgcagat
    10441 cgctctgcac acttttgtct ggcgcaaaca caatccccag gtgatttcgt gccagctcca
    10501 gcgccgattg cgtgttatcg caaacgtaat ttcccttcac gcgataatcc cgcacctctt
    10561 tactcccggc gacgttgaag cgccagatat tcgcgtcgtc gatcatcatc gagtcgatca
    10621 aaatacagga gtgatgttcg agttcgtcag gacggctaat aggatgtttc tcaagatagc
    10681 gctggctggc ataagcggtt acggcatatt gtgtaataaa actggcaacc agcgattcat
    10741 cttttggtgg agcgtagctg attaaaacat cacaatcatc aggaaattcg acgccttcat
    10801 aaaaggcatt acgctcaa
//
LOCUS       DADMEQ010000126        10714 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.127, whole genome shotgun sequence.
ACCESSION   DADMEQ010000126 DADMEQ010000000
VERSION     DADMEQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.127"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            142..1839
                     /locus_tag="JNZ05_003351"
     CDS             142..1839
                     /locus_tag="JNZ05_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAY8566464.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1973..2983
                     /gene="adhP"
                     /locus_tag="JNZ05_003352"
     CDS             1973..2983
                     /gene="adhP"
                     /locus_tag="JNZ05_003352"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HAY8566465.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVTDYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3129..3413
                     /locus_tag="JNZ05_003353"
     CDS             3129..3413
                     /locus_tag="JNZ05_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY8566466.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIVPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3463..4116)
                     /gene="fdnI"
                     /locus_tag="JNZ05_003354"
     CDS             complement(3463..4116)
                     /gene="fdnI"
                     /locus_tag="JNZ05_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HAY8566467.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4109..4993)
                     /gene="fdxH"
                     /locus_tag="JNZ05_003355"
     CDS             complement(4109..4993)
                     /gene="fdxH"
                     /locus_tag="JNZ05_003355"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY8566468.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYENAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5006..8053)
                     /gene="fdnG"
                     /locus_tag="JNZ05_003356"
     CDS             complement(5006..8053)
                     /gene="fdnG"
                     /locus_tag="JNZ05_003356"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7466..7468),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY8566469.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8285..9166
                     /gene="yddG"
                     /locus_tag="JNZ05_003357"
     CDS             8285..9166
                     /gene="yddG"
                     /locus_tag="JNZ05_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HAY8566470.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9425..10525
                     /gene="ompC"
                     /locus_tag="JNZ05_003358"
     CDS             9425..10525
                     /gene="ompC"
                     /locus_tag="JNZ05_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY8566471.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT         2628 a         2747 c         2655 g         2684 t
ORIGIN      
        1 agcattaccc aatgacgatt aaacaccatt gcctgcgcaa tggtgttttt atctgcttta
       61 tacttgggga cgacgccctg gcggtaaagc aaagacgata aaagcgtgcc agggatggat
      121 attcaaaaaa gagtgagtga catggaacca aaaacaaaaa aacagcgttc gctttatatc
      181 ccttacgctg gccctgtact gctggaattt ccgttgttga ataaaggcag tgccttcagc
      241 atggaagaac gccgtaactt caacctgctg gggttactgc cggaagtggt cgaaaccatc
      301 gaagaacaag cggaacgagc atggatccag tatcagggat tcaaaaccga aatcgacaaa
      361 cacatctacc tgcgtaacat ccaggacacc aacgaaaccc tcttctaccg tctggtaaac
      421 aatcatcttg atgagatgat gcctgttatt tataccccaa ccgtcggcgc agcctgtgag
      481 cgtttttctg agatctaccg ccgttcacgc ggcgtgttta tctcttacca gaaccgccac
      541 aatatggacg atattctgca aaacgtgccg aaccataata ttaaagtgat tgtggtgact
      601 gacggtgaac gtattctggg gcttggtgac cagggcatcg gcgggatggg cattccgatc
      661 ggtaaactgt cgctctatac cgcctgtggc ggcatcagcc cggcgtatac ccttccggtg
      721 gtgctggatg tcggaacgaa caaccaacag ctgcttaacg atccgctgta tatgggctgg
      781 cgtaatccgc gtatcactga cgatgaatac tatgaattcg ttgatgaatt tatccaggct
      841 gtgaaacaac gctggccgga cgtgctgttg cagtttgaag actttgctca aaaaaatgcg
      901 atgccgttac ttaaccgcta tcgcaatgaa atttgttctt ttaacgatga cattcagggc
      961 actgcggcgg taacagtcgg cacactgatc gcagccagcc gcgcggcagg tggtcagtta
     1021 agcgagaaaa aaatcgtctt ccttggcgca ggttcagcgg gatgcggcat tgccgaaatg
     1081 atcatcgccc agacccagcg cgaaggatta agcgaggaag cagcgcggca gaaagtcttt
     1141 atggtcgatc gctttggcct gctgacggac aagatgccga acctgctgcc tttccagacc
     1201 aaactggtgc agaagcgcga aaacctcagt gactgggata ccgacagcga tgtgttgtca
     1261 ctgctggatg tggtgcgcaa tgtaaaacca gatattctga ttggcgtctc aggacagacc
     1321 gggctgttta cggaagagat catccgtgag atgcataaac actgtccgcg tccgatcgtg
     1381 atgccgctgt ctaacccgac gtctcgcgtg gaagccacac cgcaggacat tatcgcctgg
     1441 accgaaggta acgcgctggt cgccactggc agcccgttta atcctgtggt atggaaagat
     1501 aaaatctacc ctatcgccca gtgtaacaac gcctttattt tcccgggcat cggcctgggt
     1561 gttattgctt ccggcgcgtc acgtatcacc gatgagatgc tgatgtcggc aagtgaaacg
     1621 cttgctcagt attcgccgct ggtcctgaac ggcgaaggtc tggtactacc ggaactgaaa
     1681 gatattcaga aagtctcccg cgcaattgcg tttgcggttg gcaaaatggc gcagcagcaa
     1741 ggcgtggcgg tcaaaacgtc tgctgaagct ttgcaacaag ccattgacga taatttctgg
     1801 caagccgaat accgcgacta ccgccgtacc tccatctaag cctgcacccg gtagtgaagg
     1861 ctaccgggct atttccctct ccctttttca gatctcatcc atactgggta gtggcgaata
     1921 aatctcattt gcctcacctg ctatgcagaa catcatccga aaaggaggaa ctatgaagac
     1981 tgcagttgtt acgaaggatc atcatgttga cgttacggat aaaacactgc gctcactgaa
     2041 acatggcgaa gccctgctga aaatggagtg ttgtggtgta tgtcataccg atcttcatgt
     2101 taagaatggc gattttggtg acaaaaccgg cgtaattctg ggccatgaag gcatcggtgt
     2161 ggtggcagaa gttggcccag gtgtcacctc attaaaacca ggcgatcgtg ccagcgtggc
     2221 gtggttttac gaaggatgcg gtcattgcga atactgtaac agtggtaacg aaacgctctg
     2281 ccgttcagtt aaaaatgccg gatacagcgt tgatggcggg atggcggaag agtgcatcgt
     2341 ggtcaccgat tacgcggtaa aagtgccaga tggtctggac tcggcggcgc ccagcagcat
     2401 tacctgtgcg ggggtcacca cctacaaagc cgttaaactg tcaaaaattc gtcccggtca
     2461 gtggattgct atctacggtc ttggcggtct gggtaacctc gccctgcaat acgcaaagaa
     2521 tgtctttaac gccaaagtga tcgccattga tgtcaatgat gagcagttaa aactggcaac
     2581 cgaaatgggt gcagatttag cgattaactc acgcaccgaa gacgccgcca aaattgtgca
     2641 ggagaaaacc ggtggcgctc acgctgcggt ggtaacagca gtagctaaag ctgcgtttaa
     2701 ctcggcagtt gatgctgtcc gtgcaggcgg tcgtgttgtg gctgtcggtc tgccgccgga
     2761 gtctatgagc ctggatatcc cacgtcttgt gctggatggc attgaagtgg tcggttcgct
     2821 ggtcggcacg cgccaggatc taaccgaagc cttccagttt gccgccgaag gcaaagtggt
     2881 gccgaaagtc gccctgcgtc cgttagcgga catcaacacc atctttaccg agatggaaga
     2941 aggcaaaatc cgtggccgta tggtgattga tttccgtcac taagaggtct ttgctgcgac
     3001 tcccatgttc gggtcgcagc atcgcacact ctccaacatg aaatggctaa atggattatt
     3061 gttgcgtagg aaggaacaat aatgatagtg ttcccagaga agtaacgcta ctgagagtaa
     3121 agacactaat gaaaatggcc aatcatcccc gcccggggga cattattcag gaatcactgg
     3181 acgaacttaa tgtcagcctg cgcgagtttg ccagagcaat ggaaattgtg ccctcaacgg
     3241 caagccgatt gctgaccgga aaagcagctt tgacgccaga aatggcaatt aaactttccg
     3301 tggtgatcgg cagttcgccg caaatgtggc tgaatctgca aaacgcctgg agtctggcag
     3361 aggcagaaaa aacggtggtt gtgtcgcgat tacgccgtct ggttacgcaa taaaaaatgg
     3421 caccttataa agcgccattt tcagcttaaa gttcaaaaga gattatatcc cttcttcact
     3481 ctcttttttc gcttctgcct tctcgatttc acgataccag cgcggatggt gtttcttcgc
     3541 ccagcgacgg ctcaccttcc cttcgatcat ccctttaatc gaccctttca cccagaatgc
     3601 catatacata tggatcagga tggcgtggat caggatgata cccgcagccg cgtggatcag
     3661 caggctgtag cgaacaacct gcatcgggaa gtactgcgca aagtacggac gccagataat
     3721 caccccggtc accagcagca cgaaaatcat gctcatgatc gaccagaaca tcatcttttg
     3781 cccggcgttg tacttaccaa catccgccac tttgtgctca ttgcctttta atacttcgac
     3841 aatgttcaac agccacggaa tatctttctt atccgggatg ttgtgatgca caaaacgcac
     3901 aaacataaac atcagtgcga cgaaaatcgc aatgccgaag aacgggtgca aaatgcgtcc
     3961 catctgcggc gtaccgaagg tttgcgtcag ccattgcagc gtcgggaaga agaacgaaat
     4021 cccggacagc gccaccagga agaagcaaat caccacggtc cagtgacagg cgcgatcaat
     4081 aaatttggtg cgcacaatca ttttcgactt actcatgatg atcctcctcg tcatcgtcca
     4141 cttccttatt cgggccaata ccgatgtagt ggaaaatcaa cccggcaaaa gtggcaataa
     4201 agccagccgc tgccagcggt ttcaacgcgc ctttccacag gcttaccgag gtgtcgatct
     4261 tcggatcttt cggcagaccg tgatacagct ccggctgatc ggcgtgatgc agcacgtaca
     4321 taacgtgcgt accaccgacc ccttccgggt tgtagacgcc agcattttcg taaccacgcg
     4381 ctttcagttt cgccacgcgc tgttccgcca gctccagcat ctccttcttg gtgccgaagt
     4441 ggatagcccc ggtcggacag gttttcacac aagccggttc ctggccgacg ctgacgcgat
     4501 cgacgcagag cgtgcattta tatacccggt tatcctcttt gttgaggcgc ggaatattaa
     4561 acggacaccc ggcaatgcag taaccacagc cgatgcagtt ttccgactgg aaatcgacaa
     4621 tcccgttagc gtactgaatg attgcaccag cagacgggca cgccttcagg cagcccggat
     4681 cttcacagtg catacagccg tctttacgga tcagccactc cagcttgcca ttctgttcgg
     4741 tttcgctaaa gcgcatcacc gtccaggact tggcgctcag atcggcgggg ttatcgtaaa
     4801 caccgacgca gtgccccact tcatcacgga tgtcgttcca ctccgaacac gccacctgac
     4861 aggctttaca gccgatacag gtggaaacgt cgataagttt cgcgacttct gctttgtaat
     4921 cacgcacctg agaaggcggc gtgatggagt tagttgcgga ccttttgata atatcctgcg
     4981 tttccatagc catctgttcg cccccttacg ccttctcgat gttgactaag aacgctttat
     5041 attccggcgt ttgcgagttt gcatcaccga cattcggcgt cagagtgtta gcgatataac
     5101 ctttacgcgc gacaccctca aagccccagt ggattggaat acccaccgtt tcaacctgct
     5161 gaccgtttac attcagcggt ttcagacggc gcgttaccac tgccaccgcg cggataaagc
     5221 ctcgcttgct ggagacagtg acacgatcgc cattattaat gcctttcgcc gccgcctgcg
     5281 tttcgctgat ttccacaaac tgttccggct gagcaattgc gttgagcaac gcgtgcttgg
     5341 tccaggtgtg gaagtgctcg gtcagacgat aggtcgtacc cacatacggg aactgctctt
     5401 ttttacccat ccgcagcgcg tcttgttcat acagacgaac aaccgggtta gacaccacgt
     5461 tcggatgcag cgggttagtg cccagcggcg tttcaatcgg ctcgtagtgt tccgggaacg
     5521 gaccttccgc cattttgttg atggcaaaca ggcgtcccat cccttccggc tgcataataa
     5581 acggcccggt tggcgtaccc ggtgcggcat tgccgaagtc cggaatatcg ttacccgtcc
     5641 acttgctgcc gttccactgg atcagcatcc gtttcggatc ccacggttta ccgttgatat
     5701 ccgccgaagc acggttgtag agtacgcgac ggttgagcgg ccacgcccag gcccatccca
     5761 gcgtattccc cagaccggac gggtcggagt tatcgcggtt tgccatctgg ttaccctgct
     5821 ctgtccagct accggtgtag atccagcaag aagatgcggt tgtaccgtca tcacgcagat
     5881 gcgcaaagct actcagcaac tgacctttct tcgcaatcag cacgccatta gcatcataga
     5941 gatcttccaa cgcatagccg ttgttctctt tagctacttc gtcagattgc ggttcgtgcg
     6001 gctgcttgta gttccagctc atcttcatca gcggttctac gcctttacca ccttcggcct
     6061 ggtacaactc gcgcagatga tggtagatac ccgccagaat ttcgccgtcg ttacgcgctt
     6121 cgcccggcgc gtcctgacct ttccagtgcc actgcaacca gcgaccggag ttagcgatag
     6181 aaccatcctc ttcagcaaag caggtcgaag gcagacggaa tacttcagtc tgaatagacg
     6241 ccggatcgac atcgttcgac tcgccgtggt tctgccagaa ggtagaggtt tcagtcacca
     6301 gcggatcgat aaccaccatg tacttcagtt tgctcaggca gctcaccact ttgtttttat
     6361 ccgggaagga cgcaaccggg ttaaagccct ggcagaaata accggtgact ttgccttcat
     6421 ccatcatgtt gaaatacttg atgacgtcgt aggtctggtc ccacttcggc agccagtcat
     6481 agccccagtt gttctctttc tgcgcggcat cgccatagaa agatttcatc aggctaacga
     6541 agaacttcgg atagttgctc cagtagttca cctgatcagc cagcgtcgct ttcggcgtgt
     6601 tcgcttccag atacgactgc aaatcaacct gtttttctga cggcagcgtc agataacctg
     6661 gcaggctggt agagagcaga cctaagtcag tcagcccctg aatgttggag tgaccacgca
     6721 atgcgttcac gccgccaccg gccataccca tgttaccgag cagcaactgg atcatcgcca
     6781 tagtacggat gttctgcgca cccacggtgt gctgcgtcca gcccagcgcg tacaggaagg
     6841 tggttgtgcg atccggtgcg ctggtggagg ccagcacttc acacactttc aggaagtcgg
     6901 cttttggcgt accacagatg ttttcaacaa cgtccggcgt gtagcgggaa acgtgcgctt
     6961 tcagcaggtt ccacacacag cgcggatgag tcagtgtttc atcgcgtttc gcatagccgt
     7021 tttcatcgaa ctgatagttc caggacgatt tgtcgtactg gcgtttttca gcgtcataac
     7081 cgctgaacag gccatcttcg aaagcaaaat catcacgcac cagtaggctg gcgttggtgt
     7141 aatgtttaac gtattcggcg ttgattttgt tgttttcgat caggtagcgc aaaacgccag
     7201 acaggaacgt aatgtccgta ccggaacgaa taggtgcgta aatatccgcc acagaagcgg
     7261 tacgcgtaaa acggggatcg acaacgatca aggttgcatc gttgttgttt ttcgcttcca
     7321 tcgcccagcg gaaaccgacg ggatgcgctt cagcagcgtt accgcccatt accattacga
     7381 cgttagcgtt tttgatatcc acccagtggt tggtcatagc accgcgacca aatgttggag
     7441 caagacttgc taccgttggt ccgtgtcaga cgcgcgcctg gttgtctacc gccagcatcc
     7501 cgagggagcg ggcaaatttc tgggtcagca tcccggtttc gttgctggca ccggaggcac
     7561 acagcatacc ggtagaaagc caacggttta ccgttacgcc ctgctcgttc ttttcaataa
     7621 agttagcgtc acggtcagct ttcatcagct tcgcaatacg ggagaatgct tcttcccagc
     7681 taatgcgctg ccatttgtca gaacctggcg cacgatattc cgggtagcgc agacggtttt
     7741 cactgttgac gtaatccagc aaaccggccc ctttcgggca cagcgcacca cggcttaccg
     7801 gatgatccgg gtcaccttca atgtgataaa tcgcttctct ggcgtttttt gcgccatcac
     7861 ccaggctata catcaatagc ccgcaaccta cggaacagta tgtgcaggtg ttacggatct
     7921 ctttagcgcg taataatttg tagtttcgcg cctgagccag tgcttgcttc ggggcaaagc
     7981 ccaatgccgc taccgttgtt ccagccatac ctcccgcgca gattttaaaa aattgtctgc
     8041 gactgacgtc cattgctttc ctcttttttc aggggtatta ctgcgcgagg aaactaacag
     8101 taaaattctg aatctttttg cgcgaaatca aaaagaacac gtgtttgcca caaaataatc
     8161 cgcctgcgca aattgaccta cctcaatagc ggtagaaaaa cgcaccactg cctgacaggc
     8221 cagttaaaaa aatgctataa aattcagctt aatttttaac ggcaagagag acaaaacagc
     8281 gagcatgaca cgacaaaaag caacgctcat agggctaata gcgatcgtcc tgtggagcac
     8341 gatggtagga ttgattcgcg gtgtcagcga ggggctcggc ccggtcggcg gcgcagctgc
     8401 tatctattca ttaagcgggc tgctgttaat cttcacggtt ggatttccgc gtattcggca
     8461 gattccgaaa ggctatttac tcgccgggag tctgttattc gtcagctatg aaatctgtct
     8521 ggcgctttcc ttagggtatg cggcgacccg tcatcaggcg attgaagtgg gtatggtgaa
     8581 ctatctgtgg cccagcctga caattctctt tgccattctg tttaatggtc agaaaaccaa
     8641 ctggctgatt gtaccaggat tattattagc cctggtcggc gtctgctggg tgttaggcgg
     8701 tgagaatggg ttacattatg atgaaatcat caataatatc accaccagcc cattgagtta
     8761 tttcctggcg tttattggtg cgtttatctg ggcagcctat tgcacagtaa cgaataaata
     8821 cgcacgcgga tttaacggaa ttaccgtttt tgtcctgcta acgggagcat gtctgtggat
     8881 ttactatttt ctcacgccac aaccagaaat ggtatttagc acgcccgtca tgattaaact
     8941 catctctgcg gcatttacct taggatttgc ttatgctgca tggaatgtcg gtatattgca
     9001 tggcaatgtc accataatgg cggtaggctc gtattttacg cctgtacttt cctcagcgct
     9061 tgcagccgtg ctgctcagcg ccccgctgtc gttctcgttc tggcaaggcg cgctaatggt
     9121 ctgcggcggt tccctgctct gctggctggc gacacgtcgt ggttaaaata attgccgggt
     9181 ctacggcccg gcagttaatc agataaattc cgacaatatt aaaaaataaa tttcaaatac
     9241 cagtaattca ccttatatct catttcgact tcgatcacat atcgataaat aatattattt
     9301 catctatttt tctcattgtt tttaatcacc attctatttt aaaaacagta tgtcatttat
     9361 aaatgacgcc gtttaaaatt cgtttagaaa atacgtttaa cgataattaa aaggattctg
     9421 ttaaatgaaa ttaaaaatag ttgcggtggt tgtaactggt ttgttagctg cgaacgtagc
     9481 acacgctgcc gaagtctata acaaggatgg taataaactc gacctttatg gcaaggttac
     9541 cgctctacgt tattttactg atgataagcg tgacgatggt gataaaactt atgcccgtct
     9601 cggctttaaa ggagaaacgc aaatcaatga tcaaatgatt ggttttggtc actgggaata
     9661 tgattttaaa ggctataacg atgaagccaa cggctcgcgc ggcaacaaga cccgtctggc
     9721 ctatgctggt ttaaaaatta gtgaatttgg ctctctggac tatggtcgta actacggtgt
     9781 cggctacgac attggttcat ggaccgatat gttgccagaa tttggtggcg atacctggag
     9841 tcagaaagat gtcttcatga cataccgtac caccggtcta gcaacctatc gcaactacga
     9901 tttctttggc ttaattgaag ggctgaactt tgccgcgcaa tatcaaggca aaaatgaacg
     9961 tactgacaac agtcatcttt atggtgctga ctacacgcgt gccaacggtg acggtttcgg
    10021 tatctcctca acttatgttt atgatggctt tggtatcggt gcggtatata ccaaatccga
    10081 tcggacaaat gcgcaggaaa gagccgctgc taatcctctc aatgcctccg gtaagaatgc
    10141 agaactatgg gccacaggta taaagtatga tgccaacaac atctactttg cagctaatta
    10201 cgctgaaaca ttaaacatga ccacctatgg ggatggttat atctctaaca aagcacaaag
    10261 ttttgaagtg gtggcgcaat atcaattcga cttcggcttg cgcccatcac tcgcttacct
    10321 gaaatcgaaa ggcagagatc tgggccgcta cgccgaccag gacatgattg agtatatcga
    10381 cgttggtgcg acgtatttct tcaacaaaaa tatgtcgacc tatgttgatt ataaaatcaa
    10441 cctgattgat gaaagcgact ttacccgtgc cgtagatatt cgcaccgata acatcgtcgc
    10501 tacgggcatt acctatcagt tctaacgtcc tctcctgacc agcccttctc tgggctggtt
    10561 tttgatttaa gtgctgctgt atgactcctt aagtaaaaat gagcaatttt tgatatttct
    10621 ggtcattcac ctcaattaat attattccat atcctccggg gacatcttac tataatattt
    10681 attgatgagt gattatgacc aatattaata cagc
//
LOCUS       DADMEQ010000127        10385 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.128, whole genome shotgun sequence.
ACCESSION   DADMEQ010000127 DADMEQ010000000
VERSION     DADMEQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.128"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            338..700
                     /locus_tag="JNZ05_003359"
     CDS             338..700
                     /locus_tag="JNZ05_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAY8566472.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            697..1614
                     /locus_tag="JNZ05_003360"
     CDS             697..1614
                     /locus_tag="JNZ05_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY8566473.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPRYLIKSRK"
     gene            1627..2892
                     /locus_tag="JNZ05_003361"
     CDS             1627..2892
                     /locus_tag="JNZ05_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HAY8566474.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGVISIRGLYLFYTRLPYLAMILCG
                     LIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYT
                     LSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRY
                     FVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQG
                     AELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(2959..3582)
                     /locus_tag="JNZ05_003362"
     CDS             complement(2959..3582)
                     /locus_tag="JNZ05_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8566475.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSERY
                     THTNIQVTSSGGGKVIVLLNGTTFQSAGDYQPSADRPYWITGNNCELIDIGCTFMEIT
                     SKATSVSAESVTRSGRINANGSVDIAPGVSSVNAHATGVYDVTFSHPLAAATNGYVVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLL"
     gene            complement(4324..4671)
                     /locus_tag="JNZ05_003363"
                     /pseudo
     CDS             complement(4324..4671)
                     /locus_tag="JNZ05_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569055.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
     gene            4793..5053
                     /locus_tag="JNZ05_003364"
     CDS             4793..5053
                     /locus_tag="JNZ05_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HAY8566476.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQCAKMNNSSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSN"
     gene            complement(5071..7806)
                     /locus_tag="JNZ05_003365"
                     /pseudo
     CDS             complement(5071..7806)
                     /locus_tag="JNZ05_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            complement(7807..9138)
                     /locus_tag="JNZ05_003366"
     CDS             complement(7807..9138)
                     /locus_tag="JNZ05_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8566477.1"
                     /translation="MATWQGSNGGLLAGIGGVNSNAPNVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPQDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            complement(9148..9840)
                     /locus_tag="JNZ05_003367"
     CDS             complement(9148..9840)
                     /locus_tag="JNZ05_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HAY8566478.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            complement(9843..10298)
                     /locus_tag="JNZ05_003368"
     CDS             complement(9843..10298)
                     /locus_tag="JNZ05_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HAY8566479.1"
                     /translation="MIQFKPTRNIDLIEAVGNHTDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            complement(10298..>10385)
                     /locus_tag="JNZ05_003369"
     CDS             complement(10298..>10385)
                     /locus_tag="JNZ05_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566480.1"
                     /translation="LASNGSTLTIQSNGAAFTATTIKIIAEQ"
BASE COUNT         2644 a         2444 c         2262 g         3035 t
ORIGIN      
        1 tccgctattg ccagtacacc tcaaacctac gaccttcgca ttacgaatct gtagcaccaa
       61 tcataactat ctgttttagc aagcattaac cgcattcact aagatatagt tgatggcaca
      121 aacagaaagt tgatgaatga tgttgccata tatatgtcac aaatacggca caacgatctt
      181 caaacatata gccacacatt cacagaagag cacaaagcct tgcaatccag tgcaaagatt
      241 tgtgtgccag agtttttctc atcaactacc gcaagtatcg atcgattgag acttggatga
      301 tagacttcat gcctttgatt attagctgat agaagaaatg ttaaagctat ttgcaaagta
      361 cacatctata ggcgtgctga acacacttat acactgggtg gtttttggtg tatgtatcta
      421 tgccgcgcat accaatcagg ctatggcaaa cttcgcaggt ttcgttgtgg ctgtgagttt
      481 tagcttcttt gcgaatgcaa aattcacatt caaagcatca actacaacga tgcgctacat
      541 gttatatgtc gggttcatgg gaacactgag tgctactgtt ggatgggttg ctgatagatg
      601 ttcacttcct ccaattgtca ctcttgttac cttctccgcc atcagcttgg tgtgcggttt
      661 cgtctattca aagttcattg tctttaggga tgcgaaatga agatatctct tgtcgttcct
      721 gtcttcaatg aagaagaagc gataccaatt ttttataaaa cggtacgtga attcgaagag
      781 ttgaagccat atgaagtaga aattgttttc ataaatgacg gaagcaaaga cgctacggag
      841 tcaatcatta acgctctggc tgtttctgat ccgctagttg ttccgctgtc atttacacgc
      901 aactttggta aagaaccagc attgtttgca gggttagacc atacaaccgg cgatgccgtg
      961 attccaattg atgttgacct gcaagacccg attgaggtta ttcctcatct tattgaaaag
     1021 tggcaggcag gtgctgacat ggtgcttgct aaacgttcag accgctcaac tgatggacga
     1081 ctgaaacgta agacagctga gtggttctat aaattacaca acaaaataag caccccaaaa
     1141 atagaggaaa atgtcggaga ttttcgactc atgtctcgtg aggttgtgga gaacattaaa
     1201 ctattgcctg agcgcaatct tttcatgaaa ggcatactga gctgggttgg tggtcagaca
     1261 gatgtcgttg aatatgtacg cacagagcgt gttgctggca tctcaaaatt taatggctgg
     1321 aaattatgga atctggcact ggaaggtatc acaagttttt caacctttcc tcttcgcgta
     1381 tggacttata taggcttgtt tgttgcaagc atttcatttt tatatggtgc atggatgatt
     1441 atagacacca ttgtctttgg taacccagta cgcgggtatc cctccatgct tgtatcaata
     1501 cttttcctgg gtggagtgca actgatcggg attggtgttc tcggtgaata tataggtaga
     1561 atttatttag aaacaaaatc aagaccacgg tacttaatta aaagcagaaa ataacaggat
     1621 gatcttatga agatattcat aatgcgcaat tggcacaaga tatcaatttt tatacttgca
     1681 tttactttga tttggcttag gcgcattgac attttgacaa atgcccagtt ttgggctgag
     1741 gatgcagttt tttggtataa agatgcatac gaacaaggtt ttttcagttc actcataacc
     1801 ccaagaaatg gttattttca aacagtatca acactcattg ttggtgcgac gacatttatt
     1861 aatccaattt acgcaccatt attatcaaac ttttttggaa taataattag agctataatt
     1921 atatggttct tatttaccga tagatttaaa ttcctaagca ccacatcaaa aatcttcatc
     1981 tctgtatatt taatttgcat gcccgggctt gatgaagtcc aagccaatat aacaaatgct
     2041 cactggtatt tatcattgta tgttgcaatg ataataatat ctgaagaatc caagtcaaaa
     2101 ttatggaaag cacatgattt attttttata gtattatctg gattgagcgg tccatttatt
     2161 atattcataa ttgcatcatc tgtttttaaa tatttacact tatcaaaagg agtaataagc
     2221 ataagaggtt tatacttatt ttatactcga ctgccatatt tggcaatgat cttgtgtggg
     2281 ctgatacagg caacatcaat aattcttaca tttaatggaa caagatcaca tgctccatta
     2341 ggattcagtt ttgatgttat gtcatctata atatcatcta atgtatttct tttttcattt
     2401 gtcccttgga atatcgctaa tgctggatgg gataatcacc ttttatcgta cacactatca
     2461 actttagtaa ttacattatg tgttttcatt tatattaaag gaaattggca aatgaaagtg
     2521 tttgccaccc ttccaatact tattgtcgca ttttctatgg caaaacctca actggcagat
     2581 tctgttcctc aactgccaac acttgcaacc ggaaacgggt caaggtattt tgtaaatatt
     2641 catattgcaa ttttttcatt aatatgcgtt tgtttgtttc aatgcataaa gaataaaacc
     2701 ttaaaaatat tctttaaaat ctatgtttca gtcatcctta ttgcaatgat aaaattaaac
     2761 ttttttataa cccctcttcc tgacatgaac tggtctcaag gagcagaatt aataaataaa
     2821 gcaaaacatg gagaagccgt ctcatttaca gtgctccctc catggttaac tcttgattta
     2881 ataaaaaaat aaaacttcac attaataaat aacaatgaat ccgcctccct acgtattccc
     2941 gtagggaggc tgtcgtgttt atagtagcct ggtaatgcta aatgcaaacg aactggagat
     3001 tcctgctccg ctaagcgtat tgcgcagggt caccctaaat gtatctacac caatataagt
     3061 aacatcacaa ctaactgaat caggcgcaga tataggtgtt atctgaacca cataaccatt
     3121 agtagctgct gcgagcggat gagaaaacgt tacatcatac acaccagtag catgcgcgtt
     3181 aacggatgaa acacccggcg caatgtctac actaccattg gcgttaattc tccctgagcg
     3241 cgtaactgac tctgcagaaa ccgatgtggc cttactggtt atttccataa aggtgcaacc
     3301 aatatcgatc aattcgcaat tgttccctgt tatccagtat ggtcgatctg cacttggctg
     3361 ataatcccct gcgctctgga acgttgtgcc attgagtaag actataactt ttcccccacc
     3421 tgagcttgtt acttgtatat tcgtgtgcgt gtagcgttca ctgctcacgc gatgaaaatt
     3481 tccaccgttt ataactaccg ttacaggtcg cgtcccagta ttatctatag caaggtctgc
     3541 ccccccggca ttagcctcga agtatgggtt gtttaacacc aaagcacacg aaccgttaag
     3601 gccatttatg gctaattgcg caccacctgt accggaatcc ccctgagtac catttccttc
     3661 acaagtcagg ctgttgatag tagttcctgc cccccatcta tcaccaagta taccccatcg
     3721 tgtattctgg cttgcagtta atcggttaac tgtaatggca ttaagctctg acccttcact
     3781 ggagttaagt gcgcgccagc caatacggtt accctgtaag tacacattat ctaactctga
     3841 cgttaacacc gagtctgtac gcaacccttc gccggtagtg tgcccataag acacaatgtt
     3901 ctctaattta gtgtatgctt tattggttac gagaagaccg taagacgatc ccgaaccttt
     3961 aagtgtaatg tcccccagat aatcataggc gtgtacacgt tgggttgccc cgactgtatc
     4021 accgagcatc tttaagttga aatccgaggc atggcagtta aatatcgttt ctgccatatt
     4081 ttctccggaa taatgagcac ggactcctgt cgaaccaata aatttagcgt catagttgcc
     4141 gtaatcaaga acgagtggtg acgcgatatc gtacacccct tgctttattt ttaatgttcc
     4201 accgaaaggc cacgatgtaa gcgggtcttg atatatttca ttttttattg caaatagctc
     4261 ctcttggaga tttttgccgc tattagtgcc aatgtgagca gctccttcat ctgattgaag
     4321 taactgcttt aactgatcag gatcgtactt cagcacattc tgaaaataga actgctgcga
     4381 accatacgca tcatatacag ccattgaatg gccttgctca gttacaaatt tggcaatctg
     4441 cccgttgtac acaggatatc cggcagcatt gataacaatt ggctgagaaa cagggacgtg
     4501 agatccgtct tcattctcta cataaacctg aatctggttt tcaggattta ccgggtcagt
     4561 gtcaattttt ccgatataaa ttttgccatt ggctacggct ttaaaagaac gcgccatagt
     4621 gaagagttgc gaaggcatgc tgaccacaac atttgcggcg atatctgaca tttcattgct
     4681 ccagacgaat gatatgatgc aatcatgatg tgattgcata ccgaaatggc actattgaat
     4741 atttatccag taggttacga tgccattcca cccaactggt gaggcatcaa ggatgtacag
     4801 caaatacgac gaggcacagt ttcacttgag acttccgcat gaactccacg cgaaaattaa
     4861 acagtgtgcg aagatgaata acagttcgct gaactcagag ataattgcag cgattgaaga
     4921 atcattggct aaacaaagct ctgcatcagt ttacattgac gatgcagagc gtatggcaga
     4981 acaacaatct gatatggtta agaaaattgt ctttgatacg ctcaaggagc tatataaaaa
     5041 agacagcaac taaccatccg tggctttccg ttacttctgg ttatcatcac tgttcatcca
     5101 gtacatgatg cctagccttg atatggtttt cttatctgat tctggaagtg agttgtaaaa
     5161 cgagcgccaa gcagaagagc gtcttacagt tgtatcaacg atgctttctg gtgcttgttt
     5221 tactgccagc cttgctgctt tctggaattc tggtgagcta attagctttt ctgcagattc
     5281 aattgattca gcgccgccca tcgccctgat tttgctggcg acttttgccc caatttctga
     5341 cccagcaaca gcacctactg cactgaacgg tcctgacaca gttgaaccaa ccgcagtgcc
     5401 aatgcgttgg gcatggttag caacaaattc atttgccgca gtgacgcgat taaaccgttt
     5461 gacgaactcg tttagtcttc ctgtagttat ctcgtaagat tttgcatctt tgatagcctt
     5521 tgcgacctta tatacatcgt tcagacctga catagtctct tttggtaaat gtctggcaag
     5581 attgcgcatc tgcccgtttg ctaacatgtt ttgataccag tcagcaaacc ctgcaggatt
     5641 aaaatcagcg ccacgttttc cgttcgaaag catatcccga agacctgttc ccagtagctc
     5701 ggttcttagc ttcctggacg gcgtgttttg catcaattca cgaaatcctt tggcatcgcc
     5761 tttcgacatt gcctgtagtg ctgtagttgc tttgcgagaa acatcaccgt tcagcgttct
     5821 gccagttaga ttaatcattt gctcttccat gctttttcgc atctgaacaa gcctctgagc
     5881 aacttcaaaa tcacgtaatg catttgtctc tgacagcgcc gccttttgat catcggcgag
     5941 cgaaccgtaa agcctcgata aagcagcgcg atcagcatct ttatatggtc cagaattctt
     6001 atgaagtgca tcaccaacaa gtcgtcgttg cttattgaga tttgcataag ttaatgcgcc
     6061 gttcttgcct ggattaactg cccgaaagac tctcttttca atagggtcaa ggtttttcat
     6121 cccgccaaga tcttctgcca cctgtttgag cattgacctt gttgatggcg catcaatttt
     6181 tgcacctgca ggcatcgcct tttcaacatt gcgataaagc tggtcgcttc gtttcatcag
     6241 cgcagacatt cttccattga ccgtatcaat aaacttgtcg ctcataccaa gagcatcttt
     6301 tgcaccggaa acgcgatcaa ttatctcacc cgcgccctgt gctagctgcc tgattgcttc
     6361 attttcctgc acctgtagag cagaacccgc acgggacttg attgcttgct caactgcctt
     6421 gtattgctcg ttcccggaaa agtgtgaagg gagtaatgaa tcaacattaa gcctttcagc
     6481 agactccaga acttcagcct gaggattgat atctagttca tcaagcgatg aagcgagatt
     6541 tggccttttt tgtgctgcta ctgtgcgaac gacttcctct gggttcttcg tcgcaggcgt
     6601 cattggtaca ccttgtgcta ctcttgcctc tgcggcatga accgcaggat ttgttgctgc
     6661 agcggcatca ccggagaatg gtgatgtttc tgttggcgca gtggtcgctc tctgtgcaga
     6721 tgttttattg ccactaatta tatttgatgc accacgaaca ccacgggtaa cgccatttat
     6781 caggccatgc gtagcaatgc taagtccggc attaatagcg gcattttcgg caaaatcacc
     6841 ccgttgattc gccgcgtcag cgagagaacc aatgaccatg tttccagcta cacctgctcc
     6901 aggaacaaga taaccgccta ttgactctcc agcttgcgcg taggggtctg tcggtctgtc
     6961 tactggacga taaacatcat ccaatacttt tggcccccca agcccctgac tgattgcatt
     7021 aatcagactt gcgccaccct gcaatacgtc aaatggtatg tttaccagac cacgaccagc
     7081 ctgttctgca atttaccctg cactttgacc accagtgagc caatcgccag cttgttgcat
     7141 caatgatggt tcttcccgtg ttggtgcatt attggcctga ttaactgttt gttgctgaac
     7201 aggtttcggt gcgacagaac ttacgggttg aggtggctgc tgactggctg cctgctgctc
     7261 aatctgagca aatggattat ttgggtctga ctgcacacct gatgccgata cttgttcgga
     7321 tgactgtact tccagttgtg caaacgggtt gttagggtct tgctgaggat gtacctttgc
     7381 agaggtggcg cgctgttcga ctgttgagtc tgtcaccggg tcaccagccc attgagcaaa
     7441 gcgatcatca acgtaaccgc ggccttcagg tcctggcgta tattcaccac gctttgcctt
     7501 cataacgttg ccgggaccgt cgtgataagc ctgaagagcg tcacgccagt taccaaactg
     7561 ctggtacatc tttgccagat agcgcgcgcc agcgtcagcc tgatattcgg ggttttgcat
     7621 ttgctcatcg gtataaccca tatcacgcca tgtcccaggc atgacctgag tcaatcctac
     7681 agccccggcg gagcttactg cggcagggtt gtaagaagac tccttggcac ccagtgcagt
     7741 catcaaccct tctggcacac cgtaacgtgc gccagcctgc tctaacaaat cacggtaatt
     7801 agccatttac tgccccaaag atggaagata tccgtagcga ttaatgaagt cgattgacag
     7861 ctcggggtgc tgcttcaggt aatctataga agcctgaggc gcttccactc gcttgatacc
     7921 gttttgctga acgtacttac caaccgcctc attacgcttc tggttgagcg tgttcaggat
     7981 gacgccagcg ttgcgacgaa aggactcctc gctctgcgag ttctgcagcg aaccaacagc
     8041 ctggtcgagc tttttgccct cggcatcaga aagtgcgccc atgcctcgca tggcctgaac
     8101 cgctgtcagg tatgcttggg atttaaaggt atcaagtcgt gcctgagtgt ctgcagcctg
     8161 tgagcctgga acgttgggga ttactccacg taagcctgta atgctcttaa gtgaaggaga
     8221 actaacgata tcgttcagag tgaacatgct ggttgtgagg gtgttgatgc cgtctttgta
     8281 gccatcattt agcgcttgct gcttctgctg caactgctgg ttgttggctg ctatgcggct
     8341 ctgtatttcc tggcgcttca ggtcgttagt ttctgctgat agcatccggt caaggcgctt
     8401 attttcgttg ttaatgcggt ttgtttctgc gtccagatta atgcgctgct gacctaaatt
     8461 cgcctggata tcttgcccgc gcattgtgat tgcctgattc cgagcggcgg tttgcgaatc
     8521 caggtcctga ccgcgcatgg taacctggcg accctgcatt ttatcctgta ggtcaaagta
     8581 tttttcgggt ccgagactgt tcatccccag gtgatcgaca aattcgccga actgccgcgg
     8641 gttctgttgg tacatctgag cgacgtcctg aggattaacg ccaacacgag ctaactcacc
     8701 ggcgttgttt tgcagccatg attgcattgc ttctggagac gatgacgcaa ggcgtgcgcc
     8761 agccgctaag gtgccgatag aattacgctg gtcttcatca atgaatccca tgcctttacg
     8821 aacggattca atctggtctg gatattgagt agccaactga cgcaaagcac cgcgatcacc
     8881 agacgcataa gcattagcgt acgcctgctg aaattctttc tgccgctgag cctgcttttc
     8941 ctgctgaaac acccccgcaa tacctgaaag gccttgcaaa gcagtcagcc caacattgtt
     9001 agcgcctgaa cgctcaatat cattgttctg cctgataagc tgaagcgtat tgccgatgtc
     9061 atttacgttc ggagcgtttg agttgacgcc gccgatacca gccaataatc cgccatttga
     9121 tccttgccaa gtagccatga ttacccctta aaacaacgag ccaagcaatc cgataccagc
     9181 accaatgcca gcgccccaag gtgttgatgt tcccaaaagg ctggcaagac ctgcaccggc
     9241 aatcgcacca gacgttccgc cactaattgc tgtctgaaga cttgatggtt tgttggcatt
     9301 agcagcggca agagctgcgc tttgctgtgc aatgctgctc atgttgttgg cgtatgtctg
     9361 cccggcgttt gcctgacctt gcagcgcacc aagcccaacg tttgccagat tgttgtaatt
     9421 gctcatctgg tttgataacc aagactgacc gagtgtcggc gcgatcgtag ccagttgatt
     9481 gcttgtggct gtcgaaccaa gtccacccgt cgcctccgca gcagcaagac tctggtaacg
     9541 agcctgacct gcaaggtctt tatactgctg agagttgtaa tactgattaa gtgcctgccc
     9601 ctgaccttct aaactggaaa ggttctgaag ctggttaaca tactgctccg caagaggcgt
     9661 gaacggagca aggtttttca tgatcgtctg ccactgctga ttttgcaggt ctgcggcata
     9721 cttctgagct tctgctgcat actttgcgct tttatcagag ctgccacctt tcccgccttt
     9781 ttcagggcaa taaggttcct cgccgcgcag ttttctgccc agcttaaatg catataacat
     9841 ggctatctcc cgtgattcag gaagtcgatt agttcttcgc gtgtggcgct gtaaaacgtc
     9901 acgtcatcca cgcctttgaa gtatttcttg atggttccta cacgcttaag gccaatcatt
     9961 gcgcagtaca tctgaccgtg gcggaatttg cgcgcagcga acgatgtgac gcactgaacg
    10021 gtggtgttag tcagaatgta tcgccagaac gccagcccga tttccttgct gaatccacga
    10081 atctctggca ggtacatggc gtggcaatca aaggttagcg gctgaatctc ctgatagtaa
    10141 acaattccgc cgaactgccc gtgcacgtta acctcaaagt aacggcattc aggcttgtag
    10201 tcgtatccat cgccgttgtt gctcccggcg ataatgtcag tgtgatttcc tactgcttcg
    10261 atcaggtcga tgtttcgcgt tggtttgaac tgaatcatca ctgctccgcg attatcttga
    10321 tggttgtggc agtaaacgcc gcaccatttg actgaatagt taacgtactg ccatttgagg
    10381 caaga
//
LOCUS       DADMEQ010000128        10254 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.129, whole genome shotgun sequence.
ACCESSION   DADMEQ010000128 DADMEQ010000000
VERSION     DADMEQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.129"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            80..883
                     /gene="hpaI"
                     /locus_tag="JNZ05_003370"
     CDS             80..883
                     /gene="hpaI"
                     /locus_tag="JNZ05_003370"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HAY8566481.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALTARFGNSSSISMAKQNNNSVY"
     gene            905..2260
                     /gene="hpaX"
                     /locus_tag="JNZ05_003371"
     CDS             905..2260
                     /gene="hpaX"
                     /locus_tag="JNZ05_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HAY8566482.1"
                     /translation="MTTSSLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSGIIMASMGSFTAMTIFWTTQDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            2270..3160
                     /gene="hpaA"
                     /locus_tag="JNZ05_003372"
     CDS             2270..3160
                     /gene="hpaA"
                     /locus_tag="JNZ05_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HAY8566483.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFTRLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSPEGMEHTLAALNHYWPIISLEFQQNKPG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            3268..4124
                     /locus_tag="JNZ05_003373"
                     /pseudo
     CDS             3268..4124
                     /locus_tag="JNZ05_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            4351..5909
                     /gene="hpaB"
                     /locus_tag="JNZ05_003374"
                     /pseudo
     CDS             4351..5909
                     /gene="hpaB"
                     /locus_tag="JNZ05_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            5927..6439
                     /locus_tag="JNZ05_003375"
     CDS             5927..6439
                     /locus_tag="JNZ05_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HAY8566484.1"
                     /translation="MQLDEQRLRFHDAMVSLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGESRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            6832..8982
                     /gene="btsT"
                     /locus_tag="JNZ05_003376"
     CDS             6832..8982
                     /gene="btsT"
                     /locus_tag="JNZ05_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HAY8566485.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            9032..9235
                     /locus_tag="JNZ05_003377"
     CDS             9032..9235
                     /locus_tag="JNZ05_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY8566486.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            9246..10202
                     /gene="yjiA"
                     /locus_tag="JNZ05_003378"
     CDS             9246..10202
                     /gene="yjiA"
                     /locus_tag="JNZ05_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HAY8566487.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
BASE COUNT         2443 a         2669 c         2653 g         2489 t
ORIGIN      
        1 agccccgaaa aagagccagt cagttgctta actgactggc attactccat tagctgccgc
       61 tttgtttaag gagagaacga tggaaaacag ttttaaagcg gcgctgaaag caggccgtcc
      121 gcagattgga ttatggctgg ggctgagcag cagctacagc gcggagttac tggccggacc
      181 aggattcgac tggttattga tcgacggtga gcacgcgccg aataacgtgc aaaccgtgct
      241 cacccaacta caggcgattg cgccctaccc cagccagcca gtggtacgcc catcgtggaa
      301 cgatccggtg caaatcaaac aactgctgga cgtaggcaca caaaccttac tggtgccgat
      361 ggtacaaaac gccgacgaag cccgtaaagc ggtacgcgcc acccgttatc cccccgccgg
      421 tattcgcggt gtgggcagtg cgctggctcg cgcctcgcgc tggaatcgca ttcctgatta
      481 cctgcaaaaa gccaacgatc aaatgtgcgt gctggtgcag atcgaaacgc gtgaggcaat
      541 gaagaactta ccgcagattc tggacgtgga aggggtcgac ggcgtgttta tcggcccggc
      601 ggatctgagc gccgatatgg gttatgccgg taatccgcag cacccggaag tacaggccgc
      661 cattgagcag gcgatcgtgc agatccgcga agcgggcaaa gcgccgggga tcctgatcgc
      721 caatgagcaa ctggcaaaac gctatctgga actgggcgcg ctgtttgtcg ccgtcggcgt
      781 tgacaccacc ctgctcgccc gcgccgccga agcgctgaca gcacggtttg gtaatagttc
      841 ttctatttcg atggcgaagc aaaacaacaa ctctgtctac taaacgtacg atgtggagcg
      901 catcatgacg acctctagcc tgcaatataa agataataaa actgttgaaa ttgaacatcg
      961 tgtcattaat aaattatttc gtcgcctaat catttttctt tttattctgt ttgttttttc
     1021 gtttcttgat cgcatcaata taggatttgc cggactgaca atgggcaagg atcttggcct
     1081 gacgtccaca atgtttggcc tcgccgcgac actcttttat gtcacgtacg tcatgtgcgg
     1141 gatccccagt aatatgatgt tagcgaaaat tggcgctcgt cgatggatcg ctgggatcat
     1201 ggtggtctgg ggaattgctt ccacctgcac aatgtttgca acaagccccg aaacactcta
     1261 tctactgcgt atgctggttg gtattgctga ggcgggtttt ttacctggta ttttggttta
     1321 tctgacctgg tggttcccgg catatcatcg ggccagagcc aatgccttat ttatgatagc
     1381 gatgccagtc actatgatgc tgggattgat cctttcaggc tacattctgg ccttggatgg
     1441 actgtggaat ctaaaaggct ggcaatggct ctttttgctg gaaggtctgc catcggtcat
     1501 tcttggtgtc gtcacctggt tttatctcaa cgacacacca gaccaggcga cctggcttga
     1561 tgaaaacgaa aaacaggctt taaaaacgat gattctccgt gaacaagaga gtgcatttgc
     1621 ccatgcatct gttccacgct caaccttacg tgaagtcctg accccagcca tcatgctata
     1681 tacacttgct tatttttgcc tgactaatac tctgagtgcg attaatatct ggacgccgca
     1741 aatcctacag agtttcaata ctggcagtag caacatcatg attggtttgc tggcggccat
     1801 cccccaattc tgcacaattt ttggcatgat ctggtggagt cgtcgttccg accgtcttaa
     1861 agaacgcaaa aagcatacga ttcttcccta cctgtttgcc gctgccggat ggttactcgc
     1921 ttctgcgaca gatcacagcc tcattcaact gagcggcatc attatggcct caatgggatc
     1981 atttaccgcg atgactatct tttggacaac acaggatcaa gttataagct tgcaatcgcg
     2041 tgccgttgcg atggctgtca tcaatgctgt cggcaatgta ggttcagcag tcagtccatt
     2101 acttatcggt attttgaaag atgcaaccgg cagttttagc tcaggtttgt ggtttgtagc
     2161 aggattgctg gtactgggcg cgttagtcat gacccgaatc cctatgggga gtcgggaaca
     2221 ctctctttca tctgaactta tcgcaccaaa accacaataa ggagtcatga tgtccagtga
     2281 agctatcttt gaacatggtt atgaaaatat tgatttggtc cgggaatatg atgcccgcta
     2341 cgctggtgaa gatgtgcatt atgaaacatt tacccggtta gcgaatttct ttggacgcga
     2401 tatgcgccct cactggcacg atagatattt tcaactccat tatctggtca ctggacgcat
     2461 tactttacaa ctggatgagc acttctattc tgtgcgcgca ccgctttttg tactaacacc
     2521 accatcggtg ccacatactt tctttactga tcctgatagc gatgggcatg ttctgaccgt
     2581 acgtcaggag ttaatttggc ccctggtaga aaagctctgg cctggtagtg gagatgccgc
     2641 aatgctacaa ggtatctgtc tgtcaccgga gggaatggaa catacactgg cggcattaaa
     2701 tcattattgg ccgataatat ccctggagtt tcaacaaaat aagccaggac gggaaattct
     2761 attgcaatca ctggcgcaaa gtatttttac aatcctactg cgtgaagcac caccaaatga
     2821 tatttcaaca tgcagcgtgc gcggcgagat gtgtttattc cagaagttta atcggctgat
     2881 tgacgaaaat tatcgccaac atttaatggt ccctgaatat gcaacaatgc tcggtatgag
     2941 tgaatcacgg cttaccgagc tgtgccgacg ttttgccaat caatcgccga agagattgat
     3001 tttcgaacga gtgagccggg aagccagacg gcttctgctc tatagcgagc aaagcatcaa
     3061 ccagatagcc cttgatcttg gctacaaaga ccccgcctat ttcgcccgtt ttttcaaccg
     3121 aatgatgggt tgctcaccaa cacaattccg tggtcgctga ccatttatca acctgagaga
     3181 ctcgacatga aaatatcttt acgctcacta tttgtggcgt gcctgattgc gccagtggca
     3241 ttcacctacg cctccgaacc gcaataaatg aaaattgtac tacttggaac gaaaggtggc
     3301 ccttctttat taaaagctga ccgtttaccg atggcaacgg cacttattat tggcaataaa
     3361 acctggttaa ttgatgctgg ttacggggcc agcttacaac tggtaaaaaa taatattcca
     3421 ctgcgccaga ttgataatat tttactcacc catttacaca gtgatcatat tctggactat
     3481 ccatcagtct taatgaatgc atgggctagc ggtctgcagg acaaaactat tcatgtattc
     3541 ggtccgccag gcacccgagc catgaccgct gccagttggc aggtcttcga tcgggacatc
     3601 acactgcgta tggaagaaga aggaaaaccc gatcctcgca aactcgttac agctcaagat
     3661 gttggtgaag gcgtagttta taaagatgat accgtgaccg tgactgcatt aaaggtccct
     3721 cattctcctt tccctgacgg tgaggctttt gcttatcgct tcgacacaca aggcaagcga
     3781 attgtcttct caggagatac ctcgtacttt cccccactgg caactttctc agaaggcgct
     3841 gatatacttg tgcatgaggc cgtacatgag ccctccgtcg ctaagctggc agacagtatt
     3901 ggcaatggta aaacactggc aaaagccatt gcttctcacc atacgactat tgaggatgta
     3961 ggcaaaattg ccaatgaagc aaaagtgaaa aaactcatcc ttagccacct ggtaccaatc
     4021 accgttccgg atgaaacatg gaagcaagaa gcacagaaaa ctattcaggc cctatcgtcg
     4081 ttggtcatga aaatatgacg attaccattc cattcgcccc ataaaaaatt ccgtccccgt
     4141 tagcccttgt tagcggggaa tattatgatt tgccttactg taagttgatc acgatcgcag
     4201 tttactattt aactccacct tgctgtcgaa aagtaccaac gttcgcctga accgtctctt
     4261 cccgacaacc gcgttcaacg cttcaattaa ataacaaaaa caatacacaa aattaacaat
     4321 cgtattccga ttaatactgt agaggtcgac atgaaaccag aagatttccg cgccagtacc
     4381 caacgtcctt tcaccgggga agagtatctg aaaagcctgc aggatggtcg cgagatctat
     4441 atctatggcg agcgagtgaa agacgtcacc actcatccgg catttcgtaa tgcggcagcg
     4501 tctgttgccc aactgtacga cgcgctgcac aaaccggaga tgcaggactc tctgtgttgg
     4561 aacaccgaca ccggcagcgg cggctatacc cataaattct tccgcgtggc gaaaagtgcc
     4621 gacgacctgc gccagcaacg cgacgccatc gctgaatggt cacgcctgag ctatggctgg
     4681 atgggccgta ccccagacta caaagccgct ttcggttgcg cactgggcgc gaatccgggc
     4741 ttttacggtc agttcgagca gaacgcccgt aactggtata cccgtattca ggaaactggc
     4801 ctctacttta accacgcgat tgttaaccca ccgatcgtca tttgccgacc gataaagtga
     4861 aagacgttta catcaagctg gaaaaagaga ctgacgccgg gattatcgtc agcggtgcga
     4921 aagtggttgc caccaactcg gcgctgactc actacaacat gattggcttc ggctcggcac
     4981 aagtgatggg cgaaaacccg gacttcgcac tgatgttcgt tgcgccaatg gatgccgatg
     5041 gcgtgaaatt aatctcccgc gcctcttatg agatggtcgc gggtgctacc ggctcgccat
     5101 acgactaccc gctctccagc cgcttcgatg agaacgatgc gattctggtg atggataacg
     5161 tactgatccc atgggaaaac gtgctgatct accgcgattt tgatcgctgc cgtcgctgga
     5221 cgatggaagg cggtttcgcc cgtatgtatc cgctgcaagc ctgtgtgcgc ctggcagtga
     5281 aactcgactt cattacggca ctgctgaaaa aatcactcga atgtaccggc accctggagt
     5341 tccgtggtgt gcaggccgat ctcggtgaag tggtggcgtg gcgcaacacc ttctgggcat
     5401 tgagtgactc gatgtgttca gaagcgacgc cgtgggtcaa cggggcttat ttaccggatc
     5461 atgccgcact gcaaacctat cgcgtactgg caccaatggc ctacgcgaag atcaaaaaca
     5521 ttatagaacg caacgttacc agtggcctga tctacctccc ttccagtgcc cgtgacctga
     5581 acaatccgca gatcgaccag tatctggcga agtatgtgcg cggttcgaac ggtatggatc
     5641 acgtccagcg catcaagatc ctcaaactga tgtgggacgc cattggcagc gaatttggtg
     5701 gtcgtcacga gctgtatgaa atcaactact ccggtagcca ggatgagatt cgcctgcaat
     5761 gtctgcgcca ggcacaaagc tccggcaata tggacaagat gatggcgatg gttgatcgct
     5821 gcctgtcgga atacgaccag aacggctgga ctgtgccgca cctgcacaac aacgacgata
     5881 tcaacatgct ggataagctg ctgaaataac gcagcaggag gttaagatgc aattagatga
     5941 acaacgcctg cgctttcatg acgcgatggt cagcctgtcg gcagcggtaa atattatcac
     6001 caccgagggc gacgccggac aatgcgggat tacggcaacg gctgtctgct cggtcacgga
     6061 tacaccaccg tcgctgatgg tgtgcattaa cgccaacagt gcgatgaacc cggtttttca
     6121 gggcaacggc aagttgtgcg tcaacgtcct caaccatgag caggaactga tggcgcgcca
     6181 cttcgcgggc atgacaggca tggcgatgga agagcgtttt agcctctcat gctggcaaaa
     6241 aggtccgctg gcgcagccgg tgctaaaagg ttcgctggcc agtcttgaag gagagagccg
     6301 cgatgtgcag gcgattggca cacatctggt gtatctggtg gagattaaaa acatcatcct
     6361 cagtgcagaa ggtcacggac ttatttactt taaacgccgt ttccatccgg tgatgctgga
     6421 aatggaagct gcgatttaag ctccagcccc ttcacgcatt cgtcgaaggg gcatttttat
     6481 tgtgatctca atcacatatc tacccgatga ataattcttg cccaaccgct caccgccatt
     6541 cctgaccgct tacatcccta aaataaccac tcagttattt accttacttt acgcgcacgt
     6601 atctctggca acatcactgc aggatagcgg tcaatttacc tcctcaaacg caacgcaaac
     6661 ctagaacggc ttcggcctat gattaacaaa ttactctatg gattttggtt tgcaggaagg
     6721 cagcaagtga gtgaatcccc gggagcttac aaaagtaagt gtcagaggtg aacgaacgca
     6781 gctgccgcac ctgtaagcca aaagacgacg agtaaaatgc caggttttac tatggatacg
     6841 aaaaaactat tcaagcacat accctgggtg attctcggaa tcatcggtgc attctgcctc
     6901 gcggtagttg cattacgtcg gggggagcac gtcagcgccc tgtggatcgt ggtcgcctct
     6961 gtgtcggtgt atctggtggc gtatcgctac tacagtctgt acatcgccca gaaggtgatg
     7021 aaactcgacc ccacgcgcgc gacgcctgcg gttattaaca acgacggtct gaactacgtt
     7081 ccgaccaacc gttacgtgtt gtttggtcac cacttcgccg ctatcgccgg tgctggtccg
     7141 ctggtgggac cggttctcgc cgcgcagatg ggctacctgc ctggcacgct gtggctgctg
     7201 gcgggggtag tgctggccgg tgcggtacag gactttatgg tgctgtttat ctcctctcgc
     7261 cgtaacggcg catctctcgg tgagatgatc aaagaagaga tggggccagt accggggact
     7321 atcgcgctgt ttggctgttt cttaatcatg atcatcatcc tcgccgttct ggcgctgatt
     7381 gtggttaaag ccctggccga aagtccgtgg ggtgtcttca ccgtttgctc aaccgtaccg
     7441 attgcgctgt ttatgggtat ctacatgcgc tttatccgtc cggggcgtgt gggtgaagtc
     7501 tctgttattg gtatcgtgct gctggttgcc tctatctact tcggtggcgt gattgctcac
     7561 gatccgtact ggggtccggc actgaccttt aaagacacca ccattacctt cgcgctgatt
     7621 ggctatgcgt ttgtttccgc actgctgccg gtgtggctga tcctcgcacc gcgcgactat
     7681 ctggcaacct tcctgaaaat cggcgttatc gtcggcctgg cgctgggtat cgtggtgctg
     7741 aacccggaac tgaaaatgcc tgcgatgacc cagtacattg acggtactgg cccgctgtgg
     7801 aaaggcgctc tgttcccgtt cctgttcatc accatcgcct gtggtgcggt atctggcttc
     7861 cacgcgctga tctcttccgg tacgacgccg aaactgctgg ctaacgaaac cgacgcgcgt
     7921 ttcattggct acggcgcaat gctgatggag tccttcgtgg cgattatggc gctggttgca
     7981 gcgtccatca tcgaaccggg tctttacttc gcgatgaaca ccccgcctgc tggccttggc
     8041 atcaccatgc ctaacctgca tgaaatgggt ggcgagaacg cgccgatcat catggcgcag
     8101 ctgaaagacg ttaccgcaca cgcggcagcg accgtcagct cctggggctt cgtgatttcg
     8161 ccagagcaga tcctgcaaac cgcgaaagac attggtgagc cttctgtcct gaaccgtgca
     8221 ggtggtgcgc cgacgctggc ggtaggtatc gctcacgtgt tccacaaagt gctgccgatg
     8281 gctgacatgg gcttctggta tcacttcggt attctgttcg aagccctgtt catcctgacc
     8341 gcgctggatg cgggtacccg ttctggccgc tttatgctgc aagacctgct gggtaacttc
     8401 atcccgttcc tgaaaaaaac cgactctctg gttgccggta tcatcggtac tgcgggctgt
     8461 gtgggtctgt ggggctacct gctgtatcag ggcgtggtcg atccgctggg cggcgttaag
     8521 agcctgtggc cgctgttcgg tatctccaac cagatgctgg cagccgtagc gctggtactg
     8581 ggcaccgttg tgctgattaa gatgaagcgc acccaataca tctgggtaac tgttgttccg
     8641 gctgtatggc tgcttatctg caccacctgg gcgctgggtc tgaaactgtt cagcaccaac
     8701 ccgcagatgg aaggcttctt ctacatggca agccagtaca aagagaagat tgctaacggt
     8761 actgacctga cggcgcagca gattgccaac atgaaccaca tcgttgtgaa caactacacc
     8821 aacgcaggcc tgagtattct gttcctgatt gtggtgtaca gcatcatctt ctacggtttc
     8881 aaaacctggc ttgcggtgcg taacagcgac aaacgtactg acaaagaaac accgtacgtt
     8941 ccaatcccgg aaggcggcgt gaagatctct tcgcaccact aaccgtgttt agccccgctt
     9001 cggcggggct ttgttcttat cagagtgaac tatgtttggt aacttaggac aggcaaaaaa
     9061 atatctcggc caggcggcga agatgttgat tggtattcca gactacgaca actacgtcga
     9121 gcatatgaag accaaccatc ccgacaagcc gtacatgagc tatgaagaat tcttccgcga
     9181 acgccagaac gcgcgctacg gcggcgatgg taaaggcggt atgcgctgtt gttaatggag
     9241 agagcatgaa cccgattgca gttaccctac tcaccggttt tttaggcgcg ggcaaaacta
     9301 ccctgctgcg ccatattctt aacgaacaac acggctacaa gattgccgtg attgaaaacg
     9361 aattcggcga agtctccgtt gatgatcaac tgattggcga ccgcgccacg cagatcaaaa
     9421 cgctgaccaa cggctgcatc tgctgttcgc gctccaacga gctggaggac gcactactcg
     9481 acctgctgga taatctcgac aagggcaata ttcagttcga tcgtctggtg attgaatgca
     9541 ccggcatggc cgatcccggc ccgattattc agaccttttt ctcccatgag attttatgcc
     9601 agcgctacct gctggacggc gtgattgcgc tggtggatgc ggtacatgcc gatgagcaga
     9661 tgaaccagtt caccatcgcc cagtcccagg tgggctacgc cgaccgtatt ctgctgacta
     9721 aaaccgacgt cgcaggcgaa gcagagaaac tgcgtgaacg cctggcgcgc atcaacgccc
     9781 gcgcaccggt ctacaccgtc acccacggcg acatcgacct gggcctgctg ttcaacacca
     9841 acggttttat gctggaagag aacgtcgtca gcaccaaacc gcgtttccac tttatcgcgg
     9901 ataaacaaaa cgatatttcg tcaattgtgg tggaactgga ttacccggta gatatcagcg
     9961 aagtttcccg cgtgatggaa aacctgctgc tggaatcggc ggataaactg ctgcgttaca
    10021 aagggatgct gtggattgac ggcgaaccta accgcctgct gttccagggc gtccagcgcc
    10081 tctacagcgc cgactgggac aggccgtggg gcgatgaaaa accgcatagc acaatggtgt
    10141 ttatcgggat tcagttgccg gaggaagaga ttcgggcggc gtttgcggga ttgaggaagt
    10201 aagttcggga aagatcaact ttttgagagt tccaccgcaa cattattacg agtg
//
LOCUS       DADMEQ010000129        10073 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.130, whole genome shotgun sequence.
ACCESSION   DADMEQ010000129 DADMEQ010000000
VERSION     DADMEQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10073
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.130"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(296..1156)
                     /locus_tag="JNZ05_003379"
     CDS             complement(296..1156)
                     /locus_tag="JNZ05_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HAY8566488.1"
                     /translation="MALDAGTGSIRAVIFDLEGNQIAVGQAEWRHLAVPDVPGSMEFD
                     LNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNEGTPIWACANVDARA
                     AREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSDIYRQPSTITMISDW
                     LAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADILSPVKETGTLLGVVS
                     SQAAELCGLKAGTPVVVGGGDMQLGCLGLGVVRPAQTAVLGGTFTDNKQCTLSEARHQ
                     LPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="JNZ05_003380"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="JNZ05_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HAY8566489.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="JNZ05_003381"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="JNZ05_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HAY8566490.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAGTERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSSKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAEDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="JNZ05_003382"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="JNZ05_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HAY8566491.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="JNZ05_003383"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="JNZ05_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HAY8566492.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWFWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="JNZ05_003384"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="JNZ05_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..7965
                     /gene="lsrF"
                     /locus_tag="JNZ05_003385"
     CDS             7090..7965
                     /gene="lsrF"
                     /locus_tag="JNZ05_003385"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HAY8566493.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSWIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            7989..8279
                     /gene="lsrG"
                     /locus_tag="JNZ05_003386"
     CDS             7989..8279
                     /gene="lsrG"
                     /locus_tag="JNZ05_003386"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HAY8566494.1"
                     /translation="MHVTLVEINVHEDKVEEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8318..9076
                     /gene="tam"
                     /locus_tag="JNZ05_003387"
     CDS             8318..9076
                     /gene="tam"
                     /locus_tag="JNZ05_003387"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HAY8566495.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9080..9994)
                     /locus_tag="JNZ05_003388"
     CDS             complement(9080..9994)
                     /locus_tag="JNZ05_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566496.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTYISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT         2432 a         2480 c         2652 g         2509 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgcagtaaca ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcatg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatggctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt caagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcggcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgttctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 ggaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cagcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcgg aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tagaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgc ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atccccaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggt tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gcattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcta gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgctggattg gggaatgcag
     7201 tcacgcttat cgtggatatt taatccgaaa acgggtaaaa ctgtgatgct ggcctttgac
     7261 catggttatt ttcaggggcc gactaccgga cttgaacgca ttgatataaa tatcgccccg
     7321 ctgtttgaac atgccgatgt attaatgtgt acgcgcggca ttttgcgcag cgtagttccc
     7381 ccggcgacca ataagccggt ggtactgcgg gcgtcaggtg cgaactctat tctggcggaa
     7441 ttaagtaatg aagccgtggc gttatcgatg gatgatgccg tgcgcctgaa cagttgcgcg
     7501 gtggcggcgc aggtttatat cggcagcgaa tatgaacatc agtcgatcaa aaatattatt
     7561 cagctggtcg atgccggaat gaaagtggga atgccgacca tggccgtgac tggcgtgggc
     7621 aaagatatgg tgcgcgatca gcgttatttc tcgctcgcga ctcgaatcgc cgctgaaatg
     7681 ggggcgcaaa ttatcaaaac ctattatgtc gaaaaaggtt ttgaacggat tgttgccgga
     7741 tgtccggtac ccattgttat tgctggcggt aaaaaattac cggagcgcga ggcgctggaa
     7801 atgtgctggc aggctatcga tcagggcgct tctggtgtgg atatggggcg taatattttc
     7861 cagtctgacc atccggtggc gatgatgaaa gccgtacagg cggtggttca ccataacgaa
     7921 acggctgatc gggcatatga actctatctg agtgaaaaac agtaactgcg gatttaagga
     7981 gaagaattat gcacgtcaca ctggttgaaa ttaacgttca tgaagacaag gttgaagagt
     8041 ttatcgaagt ttttcgccag aaccacctgg gctctgtaca ggaagaaggt aatttgcgct
     8101 tcgatgtctt acaggacccg gaagtgaatt cgcgctttta tatttacgaa gcctataaag
     8161 atgaagacac agtggcattc cataaaacca cgcctcacta caaaacctgt gtcgcgaaac
     8221 tggaatcttt aatgaccggg ccgcgtaaaa aacgtctgtt caatgggttg atgccgtgag
     8281 gctacaattg gggtaacgcg ctgacgggag taaaaaaatg tctgactgga acccctcttt
     8341 atatctgcac ttttccgctg aacgatcgcg tccggcggtg gagctgcttg ccagagtgcc
     8401 gctggagaat gtcgaatatg ttgccgatct tggctgtggc ccaggtaaca gcaccgccct
     8461 gctgcatcaa cgttggcctg cggccaggat aacaggcatc gactcgtctc cggcgatgat
     8521 tgctgaagcg cgcagtgctt tgccagactg ccagttcgtg gaagcagaca tccgcaactg
     8581 gcaaccggaa caggcactcg atctgatttt tgctaatgcc tcactgcaat ggctgcccga
     8641 ccactacgaa ttgtttcctc acttggtttc tttacttaat ccgcagggcg tgctggcagt
     8701 acagatgcca gataactggc tggagccgac ccatgtgctc atgcgcgaag ttgcttggga
     8761 acaaaactac ccggatcgcg ggcgtgagcc gttggctggc gttcatgctt actacgatat
     8821 tttgagcgaa gccggatgtg aggtcgatat ctggcgaacc acttactatc accagatgcc
     8881 gtcgcaccag gcgattatcg attgggtgac cgccactgga ttacgtccgt ggttacagga
     8941 tctgaccgag agcgaacagc agctttttct tacgcgctac catcagatgc tggaagagca
     9001 gtatccactg caagagaacg gacagatact gctggcattt ccgcgtctgt ttattgttgc
     9061 ccgccgtacg gagtaattat cacgtcagct ggtaatgacg atcgggaaga atttttgctg
     9121 gaatttctgc ttcatcgttc atctgtaaca ggtcaatttc aatagcgttg cagatggcat
     9181 ccagcggtaa atcattgttt tcagtaccga acggatcttc cagttcttcc gccagacaat
     9241 ccagcgaaat ataagtgtag gaaatcagca cagagataaa aggcgtcatg taatgcaggt
     9301 ccacgaccag cgcgaacggc agcatgatac aaaacagata aacggtacga tgcaaaatca
     9361 gggtgtaggc aaagggaatt ggcgtgtagg caatgcgctc acagcctgcc agaaccgctg
     9421 aaatatcatt aagccgatcg ttgaggctaa taaacaggat atctgaaagc tgtccattgc
     9481 ggcgctgaac cgccaaccat tctcccatta ttaacaagat acggttagct ggagagttcg
     9541 aagccagtac acgctgaaga tctccagtct tgagataatg agccagcact tccgcctgtg
     9601 gctgtttgcg taatgtcatg cgtaaacagt gggcgaaggc gatttgcagc ctggcaaact
     9661 cccttacact tgccgaatcc ggcaatgtcg tttttacctc tcgcagtaac gaccgtgagg
     9721 caatcatcaa ctgcccccaa agttttcgcg cttcaacgta acgggcgtac ccggcattat
     9781 tacgaaaacc aagaaaaatg gcgatggcga caccgagaat gctgaacggt gcgagggtga
     9841 atttgatgcc cagatgcgtg taccagggca gcatgaatat aacagcaata gaaaagagaa
     9901 aattgagtag taagcgcgag gatatcttgg ataatactga gccgtgccag acaaaaatac
     9961 ggcgcagcca gtgttgttgt ggacgaacaa tcatggttat cttcaggcgt ggaaaagtcg
    10021 ccctattaaa cgtgattacg atcacattct caagacgcta cttacaaatt acc
//
LOCUS       DADMEQ010000130         9955 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.131, whole genome shotgun sequence.
ACCESSION   DADMEQ010000130 DADMEQ010000000
VERSION     DADMEQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.131"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JNZ05_003389"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JNZ05_003389"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAY8566497.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JNZ05_003390"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JNZ05_003390"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HAY8566498.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3326)
                     /locus_tag="JNZ05_003391"
                     /pseudo
     CDS             complement(2698..3326)
                     /locus_tag="JNZ05_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3456..5216
                     /locus_tag="JNZ05_003392"
     CDS             3456..5216
                     /locus_tag="JNZ05_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HAY8566499.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5622..6479
                     /gene="focA"
                     /locus_tag="JNZ05_003393"
     CDS             5622..6479
                     /gene="focA"
                     /locus_tag="JNZ05_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HAY8566500.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6534..8816
                     /gene="pflB"
                     /locus_tag="JNZ05_003394"
     CDS             6534..8816
                     /gene="pflB"
                     /locus_tag="JNZ05_003394"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY8566501.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9008..9748
                     /gene="pflA"
                     /locus_tag="JNZ05_003395"
     CDS             9008..9748
                     /gene="pflA"
                     /locus_tag="JNZ05_003395"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HAY8566502.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         2552 a         2495 c         2376 g         2532 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcttggc taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata
      241 cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc caggaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat
     1501 tccgccgacc acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaattaca ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgcgttca tatgaatgat ttcactacaa cgaatgcagg catagtccgc
     2821 cgactccggc accactttgt aatgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccatta aagaactcaa
     3001 attccgtcat gttggagtta ttgagtttcg accaggccag ctcgccatca cattaccaag
     3061 cggtataaca acgccagagt gataaagacg ataagtaccg gcaccatcgg cacatcatca
     3121 taaaacgcca catctcccgc cgctgaaccc agcgtgagaa tgattaacac ttcaaacagc
     3181 gacatctgtc gcacaccgcg tcttccggtc attttgagga acaaaaaaac cagtacaaag
     3241 gtataaagac tacgtagtgc aacttcgcca aggaaatcaa aaggcacttt atcaaatgcc
     3301 atacggtgga gatcgaacgc cttcattttt atgctcttaa cgtggtttat ccgctaaaag
     3361 catagtcaac agttcatcgg catggcggcg gtgatagcgc ctttgagata aaacccgtat
     3421 tattgcgcgc tttccgtacg actaaagtga ttttcatgac gcaaacattt atccccggca
     3481 aagatgccgc tctggaagat tccatcgctc gcttccagca aaaactttca gacctcggct
     3541 ttcagattga agaggcctcc tggctgaatc ccgtgcctaa cgtctggtct gtacatattc
     3601 gcgacaaaga gtgcgcactg tgttttacca acggtaaagg cgcaaccaag aaagcggcgc
     3661 tggcttctgc actcggtgaa tatttcgagc gtctctcaac caactacttt tttgctgatt
     3721 tctggctggg cgaaaccatc gccaacggtc catttgtgca ttatcccaac gaaaaatggt
     3781 tcccactgag cgaaaatgac gatgtgccag aagggctgct cgatgaccgt ctgcgcgcat
     3841 tttacgatcc ggagaatgaa ttgaccggca gtatgctgat tgacctacaa tccggtaacg
     3901 aagatcgtgg tatttgcggt ctgccgttta cgcgccagtc cgacaatcag accgtttata
     3961 ttccgatgaa tatcattggt aacctgtacg tttctaacgg tatgtccgct ggcaataccc
     4021 gcaacgaagc acgcgttcag gggttgtccg aagttttcga acgctacgtg aaaaaccgca
     4081 ttattgctga aagcatcagc ttgccggaaa tcccggcaga cgtgctggcg cgttacccag
     4141 cggtggttga agcgatcgaa acactggaaa cagaaggttt cccgatcttc gcatatgatg
     4201 gttcccttgg cggccagtat ccggtgattt gcgtggtact gtttaatcct gcaaacggca
     4261 cctgctttgc ctctttcggt gcgcatcctg attttggcgt agcactggaa cgtaccgtga
     4321 ccgagctgct gcaaggtcgt ggcctgaaag atttggatgt gtttactccg ccaaccttcg
     4381 atgatgaaga agtcgctgaa cataccaacc tcgaaacgca ctttatcgat tccagcggtt
     4441 taatctcctg ggacctgttc aagcaggatg ccgattatcc gtttgtggac tggaatttct
     4501 ccggcaccac ggaagaagag tttgctacgc tgatggctat cttcaacaaa gaagataaag
     4561 aagtttatat tgccgattac gagcatctgg gcgtttatgc ttgccgtatt atcgtgcctg
     4621 gcatgtccga tatttatccg gctgaagatc tgtggctcgc gaataacagt atgggcagcc
     4681 atttacgtga aacgattctt tcgctaccag gcagcgagtg ggaaaaagaa gattacctga
     4741 acctcatcga gcaactggat gaagaaggtt ttgatgactt tacccgcgtg cgtgagctgt
     4801 tgggtctggc gaccgggtcg gataacggtt ggtacaccct gcgtatcggt gaattaaaag
     4861 ccatgctggc gctggctggt ggcgatctgg aacaggctct ggtctggacc gaatggacga
     4921 tggagtttaa ctcatcagta tttagtccgg aacgcgccaa ctattatcgc tgcctgcaaa
     4981 cgttgttatt actggcacag gaagaagatc gccagccgct gcaatatctg aatgcgtttg
     5041 ttcgcatgta cggcgcagat gccgtagaag ccgccagtgc ggcaatgagc ggcgaagcgg
     5101 cgttttacgg cctgcaaccg gtagatagcg atctgcacgc gtttgctgca catcagtcgt
     5161 tgttgaaggc ctacgaaaag ctgcagcgcg ccaaagcagc attctgggca aaataaaatc
     5221 aaatagccta cgcaatgtag gcttaatgat tagtctgagt tatattacgg ggcgtttttt
     5281 taatgccccg ctttacatat atttgcatta ataaaataat tgtaattata aggttaaata
     5341 tcggtaattt gtatttaata aatacgatcg atattgttac tttattcgcc tgatgctccc
     5401 ttttaattaa ctgttttagc ggaggatgcg gaaaaaattc aactcatttg ttaattttta
     5461 aaatttattt ttatttggat aatcaaatag ttactccgta tttgcataaa aaccatgcga
     5521 gttacgggcc tataagccag gcgagatatg atctatatca atttctcatc tataatgctt
     5581 tgttagtatc tcgtcgccga cttaataaag agagagttag tgtgaaagct gacaaccctt
     5641 ttgatctttt acttcctgct gcaatggcca aagtggccga agaggcgggt gtctataaag
     5701 caacgaaaca tccgcttaag actttctatc tggcgattac cgccggtgtt ttcatctcaa
     5761 tcgcattcgt cttctatatc acagcaacca ctggcacagg cacaatgccc ttcggcatgg
     5821 caaaactggt tggcggcatt tgcttctctc tggggctgat tctttgtgtt gtctgcggag
     5881 ccgatctctt tacttccaca gtgttgattg ttgttgctaa ggcgagtggg cgcatcacct
     5941 ggggtcagtt ggctaaaaac tggctaaatg tctattttgg caacctggtc ggcgcactgc
     6001 tgtttgtact tttaatgtgg ctttccggcg agtatatgac cgcaaatggt caatggggac
     6061 taaacgtcct acaaaccgcc gaccacaaag tgcaccatac ttttattgag gccgtctgtc
     6121 ttggtatcct ggcaaacctg atggtatgtc tggcagtatg gatgagttat tctggccgca
     6181 gcctgatgga caaagcgttc attatggtgc tgccggtcgc gatgtttgtt gccagcggtt
     6241 ttgagcacag tatcgcaaac atgtttatga tcccgatggg tattgtaatc cgcgacttcg
     6301 catctccgga attctggacc gctgtcggtt ctgcaccgga aaatttttct cacctgaccg
     6361 tgatgaactt catcactgat aacctgatcc cggttacgat cggtaatatt atcggcggtg
     6421 gtttgttggt tgggttgaca tactgggtca tttacctgcg tgaaaacgat caccattaat
     6481 ggttgtcgaa gtacgcagta aataaaaaat ccacttaaga aggtaggtgt tacatgtccg
     6541 agcttaatga aaagttagcc acagcctggg aaggttttac caaaggtgac tggcagaatg
     6601 aagtaaacgt ccgtgacttc attcagaaaa actacactcc gtacgagggt gacgagtcct
     6661 tcctggctgg cgctactgaa gcgaccacca ccctgtggga caaagtaatg gaaggcgtta
     6721 aactggaaaa ccgcactcac gcgccagttg attttgacac cgctgttgct tccaccatca
     6781 cctctcacga cgctggctac atcaataaag cgttggaaaa agttgttggt ctgcagactg
     6841 aagctccgct gaaacgtgct cttatcccgt tcggtggtat caaaatgatc gaaggttcct
     6901 gcaaagcgta caaccgcgaa ctggacccga tgatcaaaaa aatcttcact gaataccgta
     6961 aaactcacaa ccagggcgtg ttcgacgttt acactccgga catcctgcgt tgccgtaaat
     7021 ccggtgttct gaccggtctg ccagatgctt atggccgtgg ccgtatcatc ggtgactacc
     7081 gtcgcgttgc gctgtacggt atcgactacc tgatgaaaga caaatacgct cagttcacct
     7141 ctctgcaggc tgatctggaa aacggcgtaa acctggaaca gactatccgt ctgcgcgaag
     7201 aaatcgctga acagcaccgc gctctgggtc agatgaaaga aatggctgcg aaatacggct
     7261 acgacatctc tggtccggct accaacgctc aggaagctat ccagtggact tacttcggct
     7321 acctggctgc tgttaagtct cagaacggtg ctgcaatgtc cttcggtcgt acctccacct
     7381 tcctggatgt gtacatcgaa cgtgacctga aagctggcaa gatcaccgaa caagaagcgc
     7441 aggaaatggt tgaccacctg gtcatgaaac tgcgtatggt tcgcttcctg cgtactccgg
     7501 aatacgatga actgttctct ggcgacccaa tctgggcaac cgaatctatc ggtggtatgg
     7561 gcctcgatgg tcgtaccctg gttaccaaaa acagcttccg tttcctgaac accctgtaca
     7621 ctatgggtcc gtctccggaa ccgaacatga ccattctgtg gtctgaaaaa ctgccgctga
     7681 acttcaagaa attcgccgct aaagtgtcca tcgacacctc ttctctgcag tatgagaacg
     7741 atgacctgat gcgtccggac ttcaacaacg atgactacgc tatcgcttgc tgcgtaagcc
     7801 cgatgatcgt tggtaaacaa atgcagttct tcggtgcgcg tgcaaacctg gcgaaaacca
     7861 tgctgtacgc aatcaacggc ggcgttgacg aaaaactgaa aatgcaggtt ggtccgaagt
     7921 ctgaaccgat caaaggcgat ctcctgagct acgatgaagt gatggagcgc atggatcact
     7981 tcatggactg gctggctaaa cagtacatca ctgcactgaa catcatccac tacatgcacg
     8041 acaagtacag ctacgaagcc tctctgatgg cgctgcacga ccgtgacgtt atccgcacca
     8101 tggcgtgtgg tatcgctggt ctgtccgttg ctgctgactc cctgtctgca atcaaatatg
     8161 cgaaagttaa accgattcgt gacgaagacg gtctggctat cgacttcgaa atcgaaggcg
     8221 aatacccgca gtttggtaac aatgatccgc gtgtagatga cctggctgtt gacctggtag
     8281 aacgtttcat gaagaaaatt cagaaactgc acacctaccg tgacgctatc ccgactcagt
     8341 ctgttctgac catcacttct aacgttgtgt atggtaagaa aactggtaac accccagacg
     8401 gtcgtcgtgc tggcgcgccg ttcggaccgg gtgctaaccc gatgcacggt cgtgaccaga
     8461 aaggtgctgt agcgtctctg acttccgttg ctaaactgcc gtttgcttac gctaaagatg
     8521 gtatctccta caccttctct atcgttccga acgcactggg taaagacgac gaagttcgta
     8581 agaccaacct ggctggtctg atggatggtt acttccacca cgaagcatcc atcgaaggtg
     8641 gtcagcacct gaacgttaac gtgatgaacc gtgaaatgct gctcgacgcg atggaaaacc
     8701 cggaaaaata tccgcagctg accatccgtg tatctggcta cgcagtacgt ttcaactcgc
     8761 tgactaaaga acagcagcag gacgttatta ctcgtacctt cactcaatct atgtaattag
     8821 atttgactga aatcgtacag taaaaagcgt acaataaagg ctccacgaaa gtggggcctt
     8881 ttttagcacg agagcctttt ttgtcagcta tctatacttt aaggtgactg ccaaaacaga
     8941 ctcgacgtag ccttcgagct gcgcaccaac acggcctcag atgggccaca tctggagaaa
     9001 caccgcaatg tcagttattg gtcgcattca ctcctttgaa tcctgtggaa ccgtagacgg
     9061 cccaggtatt cgctttatca cctttttcca gggctgcctg atgcgctgcc tgtattgtca
     9121 taaccgcgac acctgggaca cgcatggcgg taaagaagtt accgttgaag atttgatgaa
     9181 ggaagtggtg acctatcgcc actttatgaa cgcttccggc ggcggcgtta ccgcatccgg
     9241 cggtgaagca atcctgcaag ctgagtttgt tcgtgactgg ttccgcgcct gtaaaaaaga
     9301 aggcattcat acctgtctgg ataccaacgg ttttgttcgt cgttacgatc cggtgattga
     9361 tgaactgctg gaagtaaccg acctggtaat gctcgatctc aaacagatga acgacgagat
     9421 ccaccaaaat ctggttggag tttccaacca ccgcacgctg gagttcgcta aatatctggc
     9481 gaacaaaaat gtgaaggtgt ggatccgcta cgttgttgtc ccaggctggt ctgacgatga
     9541 cgattcggcg catcgcctcg gtgaatttac ccgtgatatg ggtaacgttg agaaaatcga
     9601 acttctcccc taccacgaac tgggcaaaca caaatgggtg gcgatgggtg aagaatacaa
     9661 actcgatggt gttaaaccac cgaagaaaga gaccatggaa cgcgtgaaag gcattcttga
     9721 gcagtacggt cataaggtaa tgttctaatt tccccgtaaa gcggcaactt tattgagttg
     9781 ccgctttttt attccgcgct tcattctgct acagcgtttt taacacatct tatagtgcat
     9841 ctaccatatc ccgtacctga gataataacg actgataata gctcgcgtga agttcttccc
     9901 catcgaatac cactgtatta cacaagaaag tgacatcggc atcttcaata ccaag
//
LOCUS       DADMEQ010000131         9871 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.132, whole genome shotgun sequence.
ACCESSION   DADMEQ010000131 DADMEQ010000000
VERSION     DADMEQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.132"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..143)
                     /locus_tag="JNZ05_003396"
     CDS             complement(<1..143)
                     /locus_tag="JNZ05_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8566503.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYE"
     gene            575..1693
                     /locus_tag="JNZ05_003397"
     CDS             575..1693
                     /locus_tag="JNZ05_003397"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY8566504.1"
                     /translation="MPLPDFHFSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1759..2007
                     /locus_tag="JNZ05_003398"
     CDS             1759..2007
                     /locus_tag="JNZ05_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HAY8566505.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2072..2440
                     /locus_tag="JNZ05_003399"
     CDS             2072..2440
                     /locus_tag="JNZ05_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY8566506.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2534..3187
                     /gene="nfsB"
                     /locus_tag="JNZ05_003400"
     CDS             2534..3187
                     /gene="nfsB"
                     /locus_tag="JNZ05_003400"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HAY8566507.1"
                     /translation="MNIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3295..4542
                     /locus_tag="JNZ05_003401"
     CDS             3295..4542
                     /locus_tag="JNZ05_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAY8566508.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTKAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4610..5986)
                     /gene="pheP"
                     /locus_tag="JNZ05_003402"
     CDS             complement(4610..5986)
                     /gene="pheP"
                     /locus_tag="JNZ05_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HAY8566509.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGDAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGRNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMTLVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6087..9222)
                     /gene="cusA"
                     /locus_tag="JNZ05_003403"
                     /pseudo
     CDS             complement(6087..9222)
                     /gene="cusA"
                     /locus_tag="JNZ05_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
     gene            complement(9234..9775)
                     /locus_tag="JNZ05_003404"
                     /pseudo
     CDS             complement(9234..9775)
                     /locus_tag="JNZ05_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2426 a         2724 c         2386 g         2335 t
ORIGIN      
        1 ttttcataga gggcaaccca tgtcctgacc tgggttcggg ggacaccaaa acgtgccgag
       61 atgatcctgt aaccatcatc agtagtgaag tagtgattca cgacttcaag gcgcttttca
      121 aaagggtatt ttggctttga catattaggg gctattccat ttcatcgtcc aacaaaatgg
      181 gtgcagtaca gggttgatag aaatatctcc tggggcttca tctttccaga cctcagagta
      241 aacctgaaat cggaaagctt caggcacccg cagttatctt acccttcctc aacaaaatag
      301 ctgtttattt atacagcaaa tcctgtttcg gacttctcac tccccatttg atcaactgaa
      361 gctgaacccg tttttttctg catgcctcaa tcgtcaaaat tcttcgccac cagccagctc
      421 tgccataact tgccacattg aaaaagtagc caaagtgctt caggcaccat cagttcacca
      481 tcaccaaaaa aaagaaacat tccagaactt ccgcatctcg cccagccgct ccgacttcta
      541 tactgaatag aaaacgtcaa cttaagagaa acctatgcca ttacccgatt ttcatttttc
      601 tgaacctttt accctcggta ttgaactgga aatgcaggtg gttaatccgc cgggctatga
      661 cttaagccag gactcttcaa tgctgattga cgcggttaaa aataagatca cggccggaga
      721 ggtaaagcac gatatcaccg aaagtatgct ggagctggcg acggatgttt gccgtgatat
      781 caaccaggct gccgggcagt tttcagcgat gcagaaagtc gtattgcagg cagccgcaga
      841 ccatcatctg gaaatttgcg gcggtggcac gcccccgttt cagaaatggc agcgtcagga
      901 ggtatgcgac aacgaacgct atcaacgaac gctggaaaac tttggctatc tcatccagca
      961 ggcgaccgtt tttggtcagc atgtccatgt tggctgtgcc agtggcgatg acgccattta
     1021 tttgctgcac ggcttgtcac ggtttgtgcc gcattttatt gccctttccg ccgcgtcgcc
     1081 atatatgcag ggaacggata cgcgttttgc cccctcacga ccgaatattt tttccgcctt
     1141 tcctgataat ggcccgatgc cgtgggtcag taactggcaa caatttgaag ccctgtttcg
     1201 ctgtctgagt tacaccacga tgatcgacag cattaaagat ctgcactggg atattcgccc
     1261 cagtcctcat tttggcacgg tggaggttcg ggtgatggat accccgttaa cccttagcca
     1321 cgcggtgaat atggcgggat taattcaggc caccgcccac tggttactga cggaacgccc
     1381 gttcaaacat caggagaaag attacctgct gtataaattc aaccgtttcc aggcctgtcg
     1441 ctatggtctg gaaggcgtca ttaccgatcc gcacactggc gatcgtcgac cgctaacgga
     1501 agataccttg cgattgctgg aaaaaatcgc cccttcagca cataaaattg gcgcatcgag
     1561 cgcaattgaa gcactgcatc gccaggtcgt cagcggtctg aatgaagcgc agctgatgcg
     1621 cgatttcgtc gccgatggcg gctcgctgat tgggctggtg aaaaagcatt gtgagatctg
     1681 ggccggtgac taagccggga attgccttac agaagaatat ccctgacaat ggtgttttaa
     1741 cttacattta acaatgttat gaaacaccct ttagaaacct tgaccaccgc agcaggcatt
     1801 ttgctgatgg ctttcctctc ttgcctgctg ctgcccgccc ccgcactggg gctgacgctg
     1861 gcacaaaaac tggtgaccat gttccatctg atggatctta gccagcttta cactttattg
     1921 ttttgtctgt ggtttttagt gctgggcgct attgagtatt ttgttctgcg ctttatctgg
     1981 cgacgctggt tctcgctggc ggattaaacg tcgattatcg ccgaacttcg cggcatactt
     2041 tgcttattct ccttcgccta aaggaacgtt tatggataag caatcactgc acgaaacggc
     2101 gaaacgccta gctcttgagt taccctttgt cgagctttgc tggccttttg gcccggagtt
     2161 cgatgttttt aaaattggcg gcaagatttt tatgctgtcg tcggagctac gcggcgtccc
     2221 ctttatcaat ctgaagtccg atccacaaaa atccctgtta aatcagcaaa tatacccaag
     2281 cattaagcca gggtatcaca tgaataaaaa gcactggatt tcggtgtatt ccggcgagga
     2341 aatctccgaa gcgttacttc gcgatctgat caacgattcg tggaatctgg tggttgatgg
     2401 tctggctaaa cgcgatcaaa aaagagtgcg tccaggctaa agcgggaatc tatagcgcat
     2461 ttttctcgct taccatttct cgttgaacct tgtaatctgc tggcacgcaa aattacttac
     2521 acatggagtc tttatgaata tcatttctgt cgccttaaag cgtcattcca ctaaggcatt
     2581 tgatgccagc aaaaaactta ccccggaaca ggccgagcag atcaaaacgc tactgcaata
     2641 cagcccatcc agcaccaact cccagccgtg gcattttatt gttgccagca cggaagaagg
     2701 taaagcgcgt gttgccaaat ccgctgccgg taattatgtg ttcaacaaac gtaaaatact
     2761 tgatgcctcg cacgtcgtgg tgttctgtgc aaaaaccgcg atggacgatg cctggctgaa
     2821 gctggttgtt gaccaggaag atgccgatgg ccgctttgcc acgccggaag cgaaagccgc
     2881 gaacgataaa ggtcgcaagt tcttcgccga tatgcaccgt aaagatctgc atgatgatgc
     2941 agagtggatg gcaaaacagg tttatctcaa cgtcggtaac ttcctgctcg gcgtggcggc
     3001 gctgggtctg gacgcggtgc ccatcgaagg ttttgacgcc gcaatcctcg atgcagaatt
     3061 tggtctgaaa gagaaaggct acaccagtct ggtggttgtt ccggtaggtc atcacagcgt
     3121 tgaagatttt aacgctacgc tgccgaaatc tcgtctgccg caaaacacca ccttaaccga
     3181 agtgtaattc tctcttgccg ggcatctgcc cggctatttc ccctcagatt ctcctgattt
     3241 gcataaccct gtttcagccg tcatcatagg ctgctgttgt ataaaggaga cgttatgcag
     3301 gatttaatat cccaggttga agatttagcg ggtattgaga tcgatcacac cacctcgatg
     3361 gtgatgattt tcggtattat ttttctgacc gccgtcgtgg tgcatattat tttgcattgg
     3421 gtggtactgc ggaccttcga aaaacgtgcc atcgccagtt cacggctttg gttgcaaatc
     3481 attacccaga ataaactctt ccaccgttta gcttttaccc tgcaggggat tatcgtcaat
     3541 attcaggcgg tattctggct gcaaaaaggc accaaagcgg cagatattct gactacctgc
     3601 gcgcagttgt ggatcatgat gtatgcgctg ctttcagtct tctcgttgct ggatgttatt
     3661 ttgaatctgg cgcagaaatt cccggcggca tctcagttac cgctgaaagg gatatttcag
     3721 gggattaaac tgatcggcgc aatcctggtc ggcattttga tgatctcgct gctgattggt
     3781 cagtcgccag cgattctgat cagcggtctt ggtgcaatgg ctgccgtgct gatgttggta
     3841 tttaaagatc cgattcttgg tctggtggca ggtattcagc tttccgcgaa cgatatgctg
     3901 aaactgggcg actggctgga aatgccgaaa tacggcgcgg atggcgcggt gatcgatatt
     3961 gggttaacca ccgtcaaagt gcgtaactgg gacaatacca ttaccactat tcccacctgg
     4021 tctctggttt ctgactcctt taaaaactgg agcgggatgt cagcatctgg cgggcgacgt
     4081 attaagcgca gtatcagtat tgatgtcacc agtattcgtt ttcttgatga agacgaaatg
     4141 caacgtctga ataaagcgca tttgttaaag ccttatttaa ccagccgcca tcaggaaatt
     4201 aatgagtgga atcgccagca aggttctacg gagtcggtat taaacctgcg ccgaatgacc
     4261 aatattggaa cctttcgtgc ctatctgaac gaatatctgc gtaaccatcc gcggattcgt
     4321 aaagatatga ccttaatggt acgccaactg gctccaggtg ataacggttt accgctcgag
     4381 atctacgcgt ttactaacac cgtggtgtgg ctggaatatg aaagcattca ggctgatata
     4441 ttcgatcaca tatttgcgat tgtcgaagag tttggtctgc gacttcatca gtcgccaacc
     4501 ggcaatgata ttcgctctct ggcgggtgca tttaagcagt aattaaaaaa accgctctca
     4561 tcgaatggat gagagcggac atcgttttgt cggatgtagc gtgaatgcct tatttccgac
     4621 gcagcgtttt aaatgccaca aacaggaata caatccacac cggcagcagg atcgctgaca
     4681 agcgcatatc atccatcgtg cacatcagca gcaaaatcat gccgaggaag gcgatgcaaa
     4741 gatagttgcc gaacggatag agcagcgcct taaactgtgt ttcacgcccc tgacgtcgca
     4801 tcgccgcgcg aaaacgcaga tgcgccaggc agatcataat ccagttcaac agcaacgttg
     4861 ctaccaccag cgtcatcagc agaccaaacg ctttttgcgg cagcagatag ttgattaaca
     4921 ccaccagcga agtgatcgct ccggaaagca tcagcgagtt aatcggcaca ccgcgacggc
     4981 tgacgcgagt caaaaacttc ggcgcattac cctgcacaga aaggccaaac agcatgcggc
     5041 tgttagagta aaccccgctg ttatacaccg acagcgatgc caccagaatg acgaagttca
     5101 gcgcagaagc taccacattg ctgtcgagat tatggaaaat catcacaaac gggctactgt
     5161 tggatttcac ttccacccac ggatagagtg ccagtaaaac caccagtgaa ccgatataaa
     5221 acagcaggat tcgatacacc acctgattta ccgcttttgg aatgcttttt tccggatcgc
     5281 gcgcttcagc ggctgtaatc ccaatcagct ccagaccgcc gaaggagaac ataattaccg
     5341 ccagcgacaa aatcagccca ttccggccgg tggcgaagaa accaccgtag cgccagaggt
     5401 tgtcgatact ggctttctcg ccgccgtgac cagaaaacag cagccacagg ccaaagccga
     5461 tcataccgat gatcgccagc actttaatca gcgcaaacca gaactcggtt tcgccatata
     5521 agcgcacgtt caccaggtta acggcgttga tgataataaa gaaggcagca gcccaaatcc
     5581 acgttggaac atccgggaac cagtactgca tatagatgcc cgcagcggtc agctctgcca
     5641 ttcccaccag cacgaacatt acccagtagt tccagccaga gaggaagccc gcaaacggtc
     5701 cccagtattt ataggcaaag tgggcaaatg aaccggatac cggctcttcg accaccatct
     5761 cgccaagctg gcgcataatc aggaaagcga tgatcccggc gacgccgtag cccagcaata
     5821 cagccggacc cgccatctga atcgccgggc caatgccaag aaacagacca gtaccaattg
     5881 cgtcacccag cgcaatcagt tgaatatgac ggttatgtaa tccgcgatga agcgtcggct
     5941 cttgattcga cgcagtatct tccgatacgg ttgacgcgtt tttcacgcct ttcccctgtg
     6001 tgtctttttt gttgaggggc accttttaac atttagtgcc catcgtcgca agacacaatc
     6061 cacacggtta aacgaggtat cctgctttaa ttccgtaccc gatgtcggtg cagccacatc
     6121 agcttatacg ccgccgggat aataaacagc gacagtaaag gtgcggtgat catgccgcca
     6181 atcatcggtg cggcaatccg gctcatcacc tctgaaccag cccccgttcc ccacagaatc
     6241 ggcagcagac cggcgataat caccgccacc gtcatcgctt tcgggcgcac gcgcaggacc
     6301 gcgccgtgat acaacgcctc atccagcttc tgctcgctga atgtttgtgg attattcaac
     6361 gacggctcgg cctctatggc gtgacgtaaa tacatcagca tcaccacgcc aaattcggcg
     6421 gcgaccccgg caagggcaat aaagccggta cccgtcgcca cagaaagatg aaagcccatc
     6481 caccacagta accagatgcc gcccaccagc gcaaacggta cgctgctgat aatcagcaac
     6541 gcttcgccca cccgacggaa tgccagatac aacagcacga agatgatcat cagtgtcatc
     6601 ggcaccatga gtttcagctt atggttggcg cgctccagta gctcgaactg cccggagaat
     6661 gccacgctgg tgcccggttt taactgcact ttttcagcta tcgctttttg caaatcgtga
     6721 accaccgaca ccatgtcacg atcgcgggca tcgatataaa tccagctcgt cgggcgcgca
     6781 ttctcggttt tcagcatcga cggtccggta gagactttaa cgtcggccac gtctgccagg
     6841 gtgatttgct gcttcatcgg cgtcaggatc ggcaactggc gcagtgcctg cggactatcg
     6901 cgccagcttt gcggataacg cagattaatt ggataacggg caatcccttc caccgtttcg
     6961 ccaaccatcg ccccgcccac cgcagaagtc acaaacaact gcacatccgc caccgtcata
     7021 ccgtaacgtg cggctttttc acggttaatc tcaacgttga tatagcgccc accttccagc
     7081 cgctcggcaa gcgcagaagc tacgcctggc accgttcgcg ccacttcttc aatttgctca
     7141 gccatcgcgt cgatatccgc cagcacagtg ccggaaactt taatgccgat ggggctttta
     7201 atgccggttg agagcatatc gatacggtta cgaattggcg gcacccacag attcgccagc
     7261 cccggcagac gcacggtgtt atccagttcc tcaatgattt tgtccattgt catgcctggc
     7321 cgccactgat cctgtggctt aagctggatg gtcgtttcta ccatctccag cggagcagaa
     7381 tcggtggcgg tttccgcttt cccggttttg ccaaataccc gcgccacttc aggtacgctc
     7441 ataattagct tgtcggtttt ttgcagcata ctcgccgcct ctgctgcgga aatccccggc
     7501 agcgtcgatg gcatatacaa caagtcgcct tcattgatct gcggtaaaaa ttccccgcca
     7561 actttattga gcggccagag aaccgtcagc accgaaagcg ccgccaccag cagcgtggtt
     7621 ttcggccagt gcagtacttt cagcaacagc ggatgataaa cacgaatcaa aaagcgattg
     7681 agcggattac tgctttccgg cggaattttg ccacggatcc agtaacccat caggataggg
     7741 atcactacga tcgccagcag cgccgcaccc gccatcgcat acgttttggt gaacgccaac
     7801 gggccaaaca gacgcccttc ctgcccttcc agggtgaaga tcgggataaa cgacaacgtg
     7861 ataatcagca gactgataaa cagcgccggc cccacttcaa cagacgcatc ggtgatcacc
     7921 tgccagcgcg ttttattatc cagcgtggcg tcaggatgct ggtgctgcca ctcttccagc
     7981 cgtttatgcg cattctcgat catgacgata gcggcatcga ccatcgcccc gacggcaatc
     8041 gcaatgccac ccagcgacat aatattggca ttcagcccct ggaagggcat gacaataaaa
     8101 gcaatacaca accccagcgg caacgaaata atcgccacca gcgccgagcg cacatgccag
     8161 agaaacagcg cgcagactac cgccaccaca ataaactctt ccagcaactt gccgctgagg
     8221 ttgtcgatgg cccggtcaat gagctggctg cggtcgtatg ttgtaactat ctccacgcct
     8281 tccggcagac tacttttcag cgtttccagt ttgtccttca cggcggcgat cacttctcgg
     8341 gcgtttttgc cggatcgcag gatcaccacc ccgcccgcca cttcgccttc gccgtttaat
     8401 gccccggcgc atctccgggc caatctggtc cttcgcaaca tcgcgcagat aaacgggcac
     8461 gccattttca ctggctttta aaacgatgtg attaaagtcg tcgagcgttt gtagatagcc
     8521 gctggcgcgc accatatatt ctgcttccgc cagttcgatt gacgaaccgc ccgcttcctg
     8581 gtttgaagca tccagcgcgc tttttacttc ggcgagactg atgccatact gcgccaggcg
     8641 ctgaggatcg ataaccacct gatactcttt caccacaccg cctaccgacg ccacttccgc
     8701 aacgtcagga atggttttca gctcatattt gagaaaccag tcctgcaatg agcgtaaatc
     8761 ggcaagatcg tgcttaccgc tgcgatccac cagtgcatat tcatagatcc agccaacacc
     8821 cgtggcatct ggtcccagct cggcgctgac tcccgcaggc agcttaccct gtacctggtt
     8881 gaggtactcc agcacccgcg aacgcgccca gtacggatcg gtgccatctt cgaaaatgac
     8941 atacacataa gagtcgccaa actgcgagaa accacgcaca gtcttcgcgc caggcaccga
     9001 caacatggtg gtggttagcg gataagtcac ctgattttca acgatttgcg gtgcctggcc
     9061 gggatagctg gttttaataa tcacctgcac atcggagaga tccggcaacg catccactgg
     9121 cgtattaatg atggtccagg tgccccagat gctcagaaac aacgcgccca tcagcaccaa
     9181 aaaacggttc gccaccgagc gacgaataat ccattcaatc attggttatt ccctcaatgc
     9241 gcatgggtag cactttcaga gcgcatccgc tccagtgcgc cagaaatatt ggcttcggaa
     9301 tcaatcagga acaggccgcc ggaaaccacc ttttcacctt ccgccagacc agagcgtaat
     9361 gcggtgacgc cttgcgatgc ctggaaaaca gcaacgcgtt tcggtacaaa gcgcccgtcg
     9421 gcatcaacgg taatcacccg ctgttcgttg ccggtatcaa tcagcgcctc gtcggcgttg
     9481 tcgacttcca gacgcagctg cagcgtgcgg gtcgcggcat ccacgccagg tagcagcgtc
     9541 catttgcgga tggtgagtgt tttatccggt cgcgccggaa cggtgagggt aaactgcgag
     9601 gcatctttca ccagccaggc gatggactcc gggatcgcag cagtgaccca caccgggtcc
     9661 ataccctgaa ttcgcgatta cgcgcaatac ccctgagaac cgccatgaac aagggtttca
     9721 tgatttgtga ttttagttta gtacgcaatt tggtacacaa cactattttc acttcagcgg
     9781 gtagtcatca aactcaccat aacgagcatc gttgatgatg tatgtgatca ccacaaatat
     9841 cacatctgga ctagttccgt cttcatgcgc a
//
LOCUS       DADMEQ010000132         9525 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.134, whole genome shotgun sequence.
ACCESSION   DADMEQ010000132 DADMEQ010000000
VERSION     DADMEQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.134"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(437..643)
                     /locus_tag="JNZ05_003405"
     CDS             complement(437..643)
                     /locus_tag="JNZ05_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HAY8566510.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMFFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            956..1045
                     /gene="kdpF"
                     /locus_tag="JNZ05_003406"
     CDS             956..1045
                     /gene="kdpF"
                     /locus_tag="JNZ05_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HAY8566511.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1045..2717
                     /gene="kdpA"
                     /locus_tag="JNZ05_003407"
                     /pseudo
     CDS             1045..2717
                     /gene="kdpA"
                     /locus_tag="JNZ05_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
     gene            2740..4787
                     /gene="kdpB"
                     /locus_tag="JNZ05_003408"
                     /pseudo
     CDS             2740..4787
                     /gene="kdpB"
                     /locus_tag="JNZ05_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4796..5368
                     /locus_tag="JNZ05_003409"
     CDS             4796..5368
                     /locus_tag="JNZ05_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HAY8566512.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5361..8043
                     /gene="kdbD"
                     /locus_tag="JNZ05_003410"
                     /pseudo
     CDS             5361..8043
                     /gene="kdbD"
                     /locus_tag="JNZ05_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
     gene            8040..8717
                     /gene="kdpE"
                     /locus_tag="JNZ05_003411"
                     /pseudo
     CDS             8040..8717
                     /gene="kdpE"
                     /locus_tag="JNZ05_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8992..9096
                     /locus_tag="JNZ05_003412"
     CDS             8992..9096
                     /locus_tag="JNZ05_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HAY8566513.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9407..>9525
                     /locus_tag="JNZ05_003413"
     CDS             9407..>9525
                     /locus_tag="JNZ05_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY8566514.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSV"
BASE COUNT         2119 a         2394 c         2638 g         2374 t
ORIGIN      
        1 aacataacat cttattggat tagatatgtt aacaattaat tattcttaat atatcaataa
       61 agtatatatt actctcttta ctatctgttt ttattgcggt agcgatacta ataacaaaat
      121 tcggaattta tttagatgct ggaattagaa aaagggatga cggctttctc accttaccct
      181 gaagtttcac gaaagtgtgt aaggaagtat gcaatctaac tggagatttc tgatgatcta
      241 gtcgcttaga gcgacaatga aggtgaccgc atcctgggta gccctatgaa aggatactat
      301 tgaaacgtga tgctaaaaca gtgatgaaat gaataatacg atggaaacgt cttccatgtt
      361 aattatcatg gaagacgttg atgttgaaca cctaaaccag ccccctgata cgaggctggc
      421 ttaagcgcgc aggcaatcag taaaaatcac cggttgcctt ttcggcctga tccatccaca
      481 ccggtttatc gctggttttc gaccagacgc ggtgcaggta gctgtaaaag cgggcgcggt
      541 ctttccagaa aaacatgaaa ggcagcgcca gaacgcccgc tgcaaccgca tatgtacggc
      601 gtaaaaagat aagccatgca ggatattctc tgtagagttc catacttttc tccccctttt
      661 gtatctaccc ggtgaatagc accggaaaaa tgaatttgtt tatctgatga aaatagtacc
      721 gccttttgtg taattttacc actcatccga ccacttattt ttgcttattg atggtttatt
      781 tacattcaca ctgtaattaa attacacaaa agttaaatta atactaacca tcagttaaat
      841 catggccttt gccattttta tacttttttt acaccccgtc cgcagatttt tgcgaaatct
      901 ttgcagccag aattctactc ttccggtatc acttttaggc tactggaggt gcactgtgag
      961 tgcaggcgtg atagccggcg tgttgctggt gtttttatta ctgggttatt tggtttatgc
     1021 cctgatcaat gcggaggcgt tctgatggct gcgcaagggt tcttactgat tgccacgttt
     1081 tactggtatt aatggtgctg gcgcgtcctt taggcagcgg gctggcgcgg ctgattaatg
     1141 acattcctct tccaggtaca gcgggcgttg aacgtatact ctttcgttta cctggcgtct
     1201 ctgaccatga gatgaactgg aagcaatatc tttgtgccat tctcggcctg aacatgctgg
     1261 ggctggcggt gctgtttttt atgttgctcg gtcagcacta tctgccgctt aatccacagc
     1321 agttgccggg gctgtcgtgg gatctggcgc tgaataccgc cgtcagcttt gtcacgaata
     1381 ccaactggca atcttatagc ggtgaaacca cgttgagcta tttcagccag atggcaggct
     1441 taacggtaca aaactttctt tctgccgcca gcgggattgc ggtgattttt gccttcatcc
     1501 gtgcgtttac tcgccagagc atgagcacac tcgggaatgc ctgggtcgat ctgctacgca
     1561 tcacgttatg ggtgctagtc cctgtggcgt tgttaatcgc actgtttttt attcaacaag
     1621 gtgcgctgca aaactttctg ccctatcagg cagtgaatac cgttgaagga gcgcaacagc
     1681 tgttacccat gggaccagta gcttctcagg aagcgatcaa gatgctcggt actaacggcg
     1741 gtggcttctt taatgccaac tcgtcgcatc cgtttgaaaa cccaaccgca ctgaccaact
     1801 tcgtgcagat gctggcgatc ttcttgatcc caacagcgct gtgctttgcc tttggtgaag
     1861 tgacgggcga tcgccgccag gggcgcatgt tgctgtgggc gatgtcagtg atttttgtca
     1921 tctgcgtagg cgtggttatg tgggcagaag ttcagggtaa tcctcatctg ctggcgctcg
     1981 gcgcagacag cagcatcaat atggaaggta aagagagccg tttcggcgtg ctggtcagta
     2041 gcctgtttgc ggtcgtgact acggcggcgt cctgtggcgc ggtgattgcg atgcatgatt
     2101 cgtttaccgc tctcggcggc atggtgccga tgtggctaat gcaaattggt gaagtggtgt
     2161 tcggcggtgt cggttctggt ctttacggca tgatgctgtt tgtcctgctg gcggtgttta
     2221 ttgccgggct gatgattggt cgtacaccgg aatatctggg taaaaaaatc gacgtacgcg
     2281 agatgaaact gactgcactg gcgattctgg ttactccaac actggtactg atgggcgcgg
     2341 cgttggcgat gatgaccgac gccggacgta gcgccatact taaccctagc ccgcatggtt
     2401 ttagcgaagt gctgtacgcc gtgtcgtccg ccgctaacaa caacggcagc gcctttgccg
     2461 gattaagcgc caactctccg ttctggaact gtttactggc gttctgcatg tttgtcggtc
     2521 gcttcggggt gattatcccg gtgatggcaa ttgccggttc gctggtgagt aaaaagagcc
     2581 aacccgccag ctccggcacg ctgccaacgc acggcccgct gtttgttggc ctgttaatcg
     2641 gcaccgtgtt gctggttggc gcactgacct ttatccctgc cctggcgctt ggtccggtgg
     2701 cggaatatct ctcctgatga tattgagtga gcactgaata tgagtcgtaa acaactggcg
     2761 ctattcgaac caacacttgt cgttcaggcg ctgaaagaag cggtgaaaaa attaaacccg
     2821 caggcgcaat ggcgcaatcc ggtgatgttt atcgtctgga tcggcagtct gctgaccacc
     2881 tgtattagca tcgcgatggc aagcggtgcg atgcctggca atgcgctgtt tagcgcggca
     2941 attagcggct ggctgtgggt caccgtactg ttcgctaatt tcgccgaggc gctggcagaa
     3001 ggccgcagta aagcgcaggc caacagtctt aaaggggtga aaaaaactgc ctttgaccgc
     3061 aagctgcgtg agccgaaata tggcgctgcg gcggacaaag ttcctgccga tcaactgcgt
     3121 aaaggtgaca tcgtgctggt agaagctggc gatattatcc cctgcgatgg tgaagtcatt
     3181 gaaggcggcg catcggtcga tgaaagcgcc atcaccgggg aatcggcacc ggtgatccgt
     3241 gaatccggcg gcgattttgc ctccgtcacc ggcggcacgc gtattctttc tgactggctg
     3301 gtgattgagt gtagcgttaa ccccggtgag acatttctgg atcggatgat cgcgatggtg
     3361 gaaggcgcac agcgacgcaa aacaccgaac gagattgccc tgactatcct gctgattgct
     3421 ctgaccatcg tctttttacc ggcaaccgcc acgctgtggc cgttttccgc gtggggcggt
     3481 aatgccgtga gtgtaacggt actggtggcg ctgctggtct gtctgatccc aaccactatt
     3541 ggtggcctgt tgtcagcgat cggcgtcgcc gggatgagtc ggatgctggg cgcgaatgtg
     3601 attgccacca gcggacgtgc agttgaagcg gcaggtgacg ttgacgttct gctactggat
     3661 aaaaccggca ccatcacact cggtaaccgt caggcgtcgg agtttatccc cgcgcagggc
     3721 gtggatgaaa aaacgctggc tgacgccgca caactggctt cgctggctga tgaaacgccg
     3781 gaaggtcgca gtattgtgat cctcgccaag caacgtttta acctgcgcga gcgcgatgtg
     3841 cagtcgctcc atgccacctt tgtaccgttt actgcgcaaa gccggatgag cgggatcaac
     3901 atcgacaacc gcatgatccg taaaggttct gtcgatgcca ttcgccgcca tattgaggct
     3961 aacggcggtc acttccctgc cgatgttgat caaaaagtcg atcaggttgc gcgtcaagga
     4021 gccacgccgc tggtggtggt ggaaggttct cgtgtgctgg gcgttattgc gctgaaagat
     4081 atcgtcaaag gcggtattaa agagcgcttc gcccagctgc gcaaatgggc attaaaacgg
     4141 tgatgattac cggcgataac cgtctgactg ccgccgcgat agctgcggaa gcgggtgtcg
     4201 atgattttct cgccgaagcg acaccggagg ccaagctggc attgatccgt cagtatcagg
     4261 cggaaggtcg tttggtagcg atgaccggcg acagcaccaa cgatgctccg gcgctggcgc
     4321 aggcagatgt cgcggtggcg atgaactccg gcacccaggc ggcgaaagag gcgggcaata
     4381 tggtggatct cgactctaac ccgaccaagt tgatcgaggt ggtgcacatt ggcaaacaga
     4441 tgctgatgac ccgtggctcg ctgaccacct tcagcattgc caacgatgtg gcgaaatact
     4501 tcgccattat tccggcggcg ttcgcggcaa catatccgca gttaaatgcg ctgaacatca
     4561 tgcgcctgca ttcgcccgac tccgccattc tcagtgcggt cattttcaac gccttgatta
     4621 tcgtcttttt gattcctctg gcgttaaaag gcgtgagtta taaaccgctt accgcttctg
     4681 ccatgttgcg ccgtaactta tggatttacg gtctgggtgg gctgctggtg ccgtttattg
     4741 gtatcaaagt cattgattta ctgctaaccg tttgtagtct ggtgtgaggt ttaccatgag
     4801 tggattacgt ccggcattat cgacatttct ctttctgtta ttgattactg gcggcgttta
     4861 cccgctgctg accaccgcac tggggcaatg gtggtatccc tggcaggcca acggttcgtt
     4921 gattcgtgaa ggtgatacgg tgcgcggttc agcattaatc gggcaaaatt ttaccggcaa
     4981 cggctatttt catggtcgcc cgtcggcaac agcagaaatg ccctataatc cacaggcttc
     5041 tggcgggagc aatctggcag tcagtaaccc ggagctggat aaacagatag ccgcacgcgt
     5101 tgccgcatta cgggccgcta acccggacgc cagcacgaac gttccggttg aactggtgac
     5161 ggcgtcggca agcggactgg acaataacat caccccgcaa gcggcggcct ggcagatccc
     5221 acgcatcgca aaagcgcgta atctcagcgt tgaacagctc acgcagctga tcgcaaaata
     5281 cagccaacaa ccgctggtga aatatatcgg ccagccggtc gtcaacattg tcgaactcaa
     5341 tctggcactg gataagcttg atgaataacg aacccttacg tcccgacccc gaccgtctgc
     5401 tggaacaaac tgctgcgccg catcggggga agctgaaagt tttcttcagt gcctgtgcag
     5461 gcgtcgggaa gacctgggcg atgctggcag aagcccagcg actgcgggcg caagggctgg
     5521 atattgtggt tggtgtggta gaaacccatg ggcggaaaga taccgccgcc atgcttgaag
     5581 ggctggctat tctgcctctg aaacgccagg cgtaccgtgg gcggcatatc agcgagtttg
     5641 atctcgatgc cgccctcgcc cgccgtccgg cgctgatctt aatggacgaa ctggcgcaca
     5701 gtaatgcgcc aggttctcgt catcctaaac gctggcagga tatcgaagaa ctgctggaag
     5761 ctggcattga tgttttcaca accgtcaacg ttcagcatct ggaaagtctg aatgatgtgg
     5821 tcagcggcgt caccggaatt caggtacggg aaaccgtgcc cgatcctttt ttcgatgccg
     5881 ccgacgacgt ggtgctggtg gacttgcccc cggacgatct gcgccagcgg ctgaaagaag
     5941 gcaaagtcta tattgccggg caggcggagc gcgccattga acattttttc cgcaaaggca
     6001 atctgatcgc cctgcgcgaa ctggcactgc gccgtactgc cgatcgcgtt gatgagcaaa
     6061 tgcgcgcctg gcgggggcat cctggcgaag agaaagtctg gcacacgcgc gacgcgatcc
     6121 ttttatgcat cggccataac accggcagcg aaaactggtc cgcgcggcgg cgcggctggc
     6181 gtcacggctg gggagcgtct ggcacgcggt gtatgttgaa ccccctgccc tgcatcgctt
     6241 accggagaaa aaacgtcggg caattctcag cgccttacgt ctggcgcagg agctgggcgc
     6301 ggagacggca acactttctg atccagcaga agagaaagcg gtagtgcgtt atgcccgtga
     6361 acataatctc ggcaagatta ttctcggtcg cccagcctcg cgccgctggt ggcgtcggga
     6421 aacatttgct gaccgactgg cgcgcatcgc ccccgatctc gatcaggtgc tggtcgcgct
     6481 tgatgaacca cccgcccgca cgattaacaa cgcgccggat agccgctcat ttaaagacaa
     6541 gtggcgggta cagattcagg gatgcgtggt tgccgccgcg ttatgcgccg ttatcacctt
     6601 aattgccatg cagtggctga tggcgtttga tgccgccaac ctggtgatgc tgtatctgct
     6661 tggcgtggtg gtggtggcgc tattttatgg acgctggcct tcggtggttg ccaccgtcat
     6721 taatgtggtg agtttcgatc tcttttttat cgccccacgc ggcacgctcg ccgtctctga
     6781 tgtgcaatat ctgctgacct tcgcggtgat gttaaccgtc gggctggtga tcgggaacct
     6841 tactgctggc gtgcgttatc aggcgcgggt agcccgttac cgcgagcaac gcacacggca
     6901 cttatatgaa atgtcgaaag ctctggcggt gggccgcagt ccgcaggaca tcgccgccac
     6961 cagcgaacaa tttattgcct ctacgtttca tgcccgcagt caggtgttgt tgcccgatga
     7021 caacggtaaa ttgcagccgt taacacatcc gcaaggaatg acgccatggg acgatgccat
     7081 cgcgcagtgg agttttgata aaggcctgcc tgcgggcgcg ggcaccgaca cgttacccgg
     7141 tgtaccgtac cagattttgc cgctaaaaag cggcgagaaa acctacggct ggtggtggtg
     7201 gagccgggga atctgcgcca gttgatgatc ccggaacagc agcgcctgct ggagactttt
     7261 acgctgttag tcgccaatgc ccttgagcgg ctgacgctaa ccgccagcga agaacaggcg
     7321 cggatggcaa gcgaacgtga acagatccgc aacgccctgc tggcagcgct ttcgcatgat
     7381 ttacgcacgc cgcttacggt gctgtttggt caggcagaaa tcttaacgct cgatctggca
     7441 agcgaaggat caccccacgc ccgccaggcc agcgagattc gtcagcatgt gctgaacact
     7501 acccgactgg tgaataatct actggatatg gcgcgaattc agtccggcgg ctttaatttg
     7561 aagaaagagt ggttaacgct ggaagaagta gtcggcagcg cgctgcaaat gctggaaccg
     7621 ggtttatcgt cgcccatcaa tctttctctg ccagaaccgc tgaccttaat ccacgttgac
     7681 gggccactct ttgaacgggt gctgattaat ctgctggaga acgcggtgaa atatgcgggt
     7741 gcgcaggccg aaattggtat cgatgcccac gttgagggcg aaaatctaca actggatgtc
     7801 tgggataacg gccccggtct tccgccaggc caggagcaga cgatatttga taagtttgct
     7861 cgcgggaata aagagtcggc ggtgccgggt gtcgggcttg ggttggcaat ttgtcgggcg
     7921 attgtggatg tacacggcgg caccattacc gcgttcaacc gaccggaagg tggtgcctgt
     7981 tttcgtgtta cacttcccca gcaaaccgct cctgaacttg aagactttca tgaggatatg
     8041 tgacaaacgt tctgattgtt gaagatgaac aggctattcg tagctttctg cgcacggcgc
     8101 tggagggcga cgggatgcgc gtctttgagg ccgaaactct gcaacgcggc ttgctggaag
     8161 cggcaacccg taagccagat ttgattattc tcgatctcgg cctgcccgat ggtgatggga
     8221 ttgagtttat ccgcgacctg cgccagtgga gcccggtgcc ggtgattgtg ctttccgcac
     8281 gcagctaaga gagcgacaag atcgccgcgc tggatgccgg agcggatgat tatctgagta
     8341 agccgtttgg cattggcgaa ttgcaggccc gtctgcgcgt cgctttacgc cgccactccg
     8401 ccaccgccgc gcccgatccg ctggtgaaat tttccgatgt taccgtcgat ttagccgccc
     8461 gcgtgattca ccggggtgag gaagaggtgc atctcacacc aattgagttc cgcctgctag
     8521 cggtgctgct caacaatgcc ggaaaagtac tcacccagcg ccagctcctt aaccaggtgt
     8581 gggggccaaa cgcggtcgaa cacagtcact atttgcgtat ttatatggga catctgcgac
     8641 aaaaactgga acaggatccg gcccggcctc gccacttcat tactgaaacc ggtattgggt
     8701 atcggtttat gctttgaatt gttattcaat aaattcgctt aaatagtgat taatttatta
     8761 ttaaacctgc gccgaatatt attatttggc aggtttaatt tcttaacgca aatataaaac
     8821 acgaaattac aacatttaag taacaaaaca gcatctaata tggcgatatt cataattaat
     8881 gatttattat ctggggtatg atcgtttttt gttgatcttc ttcacagatt atagccattt
     8941 catggataga ataactctac cttcaactga cacagcaagc ggtaaaggta aatggaaaat
     9001 aataaccgct taatgcctca tataaggcgg acaacccata tcatgaagtt tgcccatcgt
     9061 aatagttttg actttcattt ctttaatgcc cgctagtctt ctgactaaag ggcaccccaa
     9121 cgtacaggtc tccctgactt taagcattac aggttaatac ctgtattcct cggtgctcat
     9181 atactgctaa cccttttaaa ctctaaataa ttcgagtcgc agcacttgca acttgaggta
     9241 tgacgagtat agccagttac cgggctggtc tgggttattg catctgcaaa aagcaaacta
     9301 ctgatttatt tatcagcggt ggagctttgc tttttttccg gcgtgatcga tttctccttt
     9361 gagaaattga ggacctgcta ttacctgaaa taaagagatg aataaaatgt cagaattaaa
     9421 aattgccgtt agccgttctt gcccggattg tttttccact catcgtgcat gcgtgaatat
     9481 tgacgaaagt aattatattg acgttgccgc cattatttta tcagt
//
LOCUS       DADMEQ010000133         9371 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.135, whole genome shotgun sequence.
ACCESSION   DADMEQ010000133 DADMEQ010000000
VERSION     DADMEQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.135"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..1631
                     /locus_tag="JNZ05_003414"
     CDS             <1..1631
                     /locus_tag="JNZ05_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HAY8566515.1"
                     /translation="DGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFANNT
                     VTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYSGAIYSSGTNDTGAVDLRVTNAMF
                     RNNIANDGKGGAIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHP
                     SGYTIINNTAFTNNTAEGYGGAIYTNSVRAPYLIDISVDDSYSQNGGVLVDENNSAAG
                     YGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDL
                     VLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQA
                     ELNVGSTQQTFVHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVL
                     AGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGT
                     SYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNS
                     YLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDK"
     gene            1644..3575
                     /locus_tag="JNZ05_003415"
     CDS             1644..3575
                     /locus_tag="JNZ05_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY8566516.1"
                     /translation="MEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMAD
                     SSGQHQDKGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGDG
                     ATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATDG
                     VTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGTL
                     LFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQN
                     NAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDPT
                     PDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHYT
                     AAGQLAQQEDTSTVLLSGGLFSGCWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADNQ
                     NHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLEA
                     GYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTAV
                     GVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAWQ
                     KGSDDFAQTAGFLSMTVKW"
     gene            complement(3703..4425)
                     /locus_tag="JNZ05_003416"
     CDS             complement(3703..4425)
                     /locus_tag="JNZ05_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HAY8566517.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            4572..7199
                     /gene="gyrA"
                     /locus_tag="JNZ05_003417"
     CDS             4572..7199
                     /gene="gyrA"
                     /locus_tag="JNZ05_003417"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HAY8566518.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7348..9036
                     /locus_tag="JNZ05_003418"
     CDS             7348..9036
                     /locus_tag="JNZ05_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HAY8566519.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRLRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWCREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            9033..>9371
                     /locus_tag="JNZ05_003419"
     CDS             9033..>9371
                     /locus_tag="JNZ05_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HAY8566520.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYV"
BASE COUNT         2303 a         2280 c         2625 g         2163 t
ORIGIN      
        1 ccgatgggca atggctggtt ttttcggata tgaccaataa tgccagcggt ggggccgtat
       61 ttttgcaaca aggagcggaa ttttcactat taccagaaaa tgaaactgga atgactctgt
      121 ttgccaataa caccgttaca ggagaatata ataacggcgg ggccatattt gctaaagaaa
      181 actcaacgct gaatcttact gatgttattt tttccggtaa cgtcgcaggc ggctatagtg
      241 gcgcaatcta ttcttctggt actaatgata ctggtgccgt cgatttacgt gtcactaacg
      301 ccatgtttcg caataatatc gctaatgatg gcaaaggtgg cgcaatttat accattaata
      361 atgacgtcta tttaagtgat gatgttttta acaataacca ggcatataca tcaacaagtt
      421 acagtgatgg cgatggcggg gcaatcgatg ttaccgataa taatagcgac agcaagcatc
      481 cttcaggtta tacgataata aataacaccg cctttacaaa taacacagcc gaaggttatg
      541 gcggggcgat atataccaat agcgtgaggg ctccctatct tattgatatt tctgttgatg
      601 acagctacag ccagaacggc ggcgtgttag tcgatgagaa caatagcgca gcaggctatg
      661 gagatggtcc ttccactgcg gcgggtggct ttatgtatct cggcttaagt gaagttacct
      721 ttgatattgc cgacggaaaa acgctggtta tcggcaatac agagaatgac ggagctgttg
      781 actctattgc tggtaccggg ttaatcacca aaacaggttc cggcgatctg gtacttaatg
      841 cagataacaa tgactttact ggtgagatgc agattgaaaa cggtgaagtt accctgggtc
      901 gcagcaactc cctgatgaat gtcggcgata cgcattgcca ggacgatccg caagactgct
      961 acggtctgac gatagggagt attgatcagt atcagaatca ggctgagcta aacgttggct
     1021 cgacccaaca aacttttgtg cacgcattga cgggctttca gaatggcact ttaaatatcg
     1081 atgctggtgg caacgttact gttaatcagg gcagttttgc tggcatcatc gaaggtgctg
     1141 gtcagctcac cattgcgcaa aacggcagct acgtgctggc aggggcgcag tcgatggcgc
     1201 taaccggcga tatagtcgtt gatgatggtg cggtgctttc gctggaaggc gacgcggcag
     1261 atcttaccgc tctccaggac gatccgcagt cgatcgtgtt aaacggcggt gtgctcgatc
     1321 tctctgattt ctccacttgg cagagcggca catcatacaa tgatggcctt gaagtcagtg
     1381 gcagcagcgg aacggttatc ggcagtcagg atgtggtaga tcttgcaggt ggcgacaatt
     1441 tgcatatcgg cggtgacggg aaagatggcg tctacgtggt ggtcgatgcg agcgacgggc
     1501 aggtaagtct ggcaaacaat aatagttatt tgggcacaac acaaatcgcc tccggtacgc
     1561 tgatggtgag cgacaactcg cagcttggag atacccacta taaccgccag gttatcttta
     1621 ccgataagta acaagaaagc gtgatggaga ttactgccaa tgtcgatact cgctctacga
     1681 cgactgagca tgggcgtgat attgaaatgc gcgccgacgg tgaagtggca gttgatgcgg
     1741 gggtagacac gcagtggggc gcactgatgg ctgacagcag cgggcagcat caggataagg
     1801 gtagcacatt tactaaaacg ggggcgggta cgctggagct gaccgccagc ggtacaacgc
     1861 agtcggcggt acgtgtcgaa gaaggcaccc tgaaaggcga tgttgcggat atctttcctt
     1921 atgcttcgtc actgtgggtt ggtgatgggg caacgttcgt tactggcgcg gatcaggata
     1981 ttcagtcaat tgatgctact tccagcggca ctatcgacat cagcgatggt acggttttgc
     2041 gtctgaccgg gcaggatact tccgtcgccc ttaatgcctc actatttaac ggcgatggga
     2101 cgctggtaaa tgctaccgat ggcgtgacgt tgacaggcga gcttaatacc aaccttgaaa
     2161 ctgacagcct gacctatctt gctgacgtga cggttaatgg tgatctgacc aatacgtccg
     2221 gtgcggttag cctgcaaaat ggcgtcgctg gcgatacgct gacggtaaac ggtgattata
     2281 ccggcggcgg tacgctacta ttcgacagcg aattaaacgg cgatgactcg gtaagcgatc
     2341 aattggtgat gaacggtaat actgctggca acacaactgt ggtggttaac tccattacag
     2401 ggattggtga gccgacatcg acaggcatta aagtggttga tttcgcagct gatcccacgc
     2461 agtttcaaaa caatgcacag ttcagtctgg ctggcagtgg ctacgtcaac atgggagcgt
     2521 atgactacac gctagtggaa gataacaacg actggtatct gcgatcgcaa gaagtaacgc
     2581 cgccatcgcc acctgagcca gacccgactc ccgatcctga tcccacgccg gatcctgacc
     2641 caacccccga cccggcccct acgcctgctt accagccggt gctgaatgcc aaagttggcg
     2701 gttatctcaa taacctgcgg gcggcaaatc aggcgtttat gatggagcga cgcgatcacg
     2761 ctggtggcga tggtcagacg ctgaatttac gtgttatcgg cggacgttat cattacacgg
     2821 cggcagggca actggctcag caggaagaca cttctacggt gctgcttagc ggcggcttgt
     2881 ttagcgggtg ctggggcacg gatggcgagt ggatgcttgg ggcggttggt ggctacagtg
     2941 ataatcaggg cgacagccgc tcgaatatga ccggaactcg cgccgataac cagaaccacg
     3001 gttatgccgt tgggctgacc tcaagctggt atcagcacgg taatcagaag cagggggcct
     3061 ggctggatag ctggctgcaa tacgcgtggt ttaacaatga tgtttccgaa catgaagatg
     3121 gcgtggatcg ttaccattcg tcggggatta tcgcctcgct ggaagccggg tatcagtggt
     3181 taccggggcg tggtgtggtg attgaaccgc aggcgcaggt gatttatcag ggcgtgcagc
     3241 aggatgattt taccgccgct aaccatgcgc gcgtgtcaca atcgcagggg gatgatattc
     3301 agacgcggct gggtttacac agcgaatggc gtaccgcggt tggtgtcaca ccaacattag
     3361 atctgaatta ttatcacgat ccccatgcga cggaaattga agaagatggc agcactatca
     3421 gtgacgatgc ggtgaagcta cggggtgaaa taaaagtggg agtcacgggc aatatcagtc
     3481 agcgagtgtc tctgcgcggc agcgtggcgt ggcagaaagg gagtgatgat tttgcccaga
     3541 cggcagggtt tttgtcgatg acggtgaaat ggtaatcgct gtctgaccat cacaattgcc
     3601 ggatgcggcg taaacgcctt atctggccta cggagagcat ttgtaggcct gataagacgc
     3661 tgcaagcgtc gcatcaggca ttggcgcggc caaacatcaa cctcacttat tctgcgtgtg
     3721 cagcatatag ttcacatcca cgccggggcc gagcttaaag gaattagtga tcgggttgta
     3781 atgtagcccg gttatatgtc gctcttttaa actggtctga tccacccagc caagcagctc
     3841 tgccggttta ataaacttct tcacatcatg cgtacctttg ggcaccatgc gcaaaatata
     3901 ttccgcgcca accaccgcca tcagccatga cttgccgttg cggttaagcg tcgagaaaaa
     3961 gacatcgccg cctggtttca ccagttgcgc acaggctctg accactgact gcggatcggg
     4021 tacgtgctcc agcatctcca tgcaggtcac cacatcatac tgcccggcat gttttgccgc
     4081 gtgctcttcc acggtttcct gcacgtaatc cacctgaatg ccgctttcca gcgcgtgcag
     4141 ctttgccacc tgcaatggct caaagcccat atccagaccg gtcaccgtcg cgccttcgcg
     4201 cgccatactc tcggccagaa tgccgccgcc acaaccgaca tcgagcacct ttttgccaaa
     4261 taaaccgcca gcacgctcgg caatatagcc cagacgcagc gggttaatgc ggtgcaacgg
     4321 tttgaactca ccttcaagat cccaccagcg ggaggcgacg gcttcaaatt tagcgatctc
     4381 ttcgtggtct acgttatggt tttccggcga tttttcggca ttcattggca cttctactcc
     4441 gtaattggca agacaaacga gtatatcagg cattggatgt gaataaagcg tataggttta
     4501 cctcaaactg cgcggctgtg ttataatttg cgacctttga atccgggata cagtagaggg
     4561 atagcggtta gatgagcgac cttgcgagag aaattacacc ggtcaacatt gaggaagagc
     4621 taaagagctc ctatctggat tatgcgatgt cggtcattgt tggccgtgcg ctgccagatg
     4681 tccgagatgg cctgaagccg gtacaccgtc gcgtacttta cgccatgaac gtactaggca
     4741 atgactggaa caaagcctat aaaaaatctg cccgtgtcgt tggtgacgta atcggtaaat
     4801 accatcccca tggtgactcg gcggtttatg acacgatcgt ccgtatggcg cagccattct
     4861 cgctgcgtta catgctggta gacggtcagg gtaacttcgg ttccatcgac ggcgactctg
     4921 cggcggcaat gcgttatacg gaaatccgtc tggcgaaaat tgcccatgaa ctgatggccg
     4981 atctcgaaaa agagacggtc gatttcgttg ataactatga cggcacggaa aaaattcccg
     5041 acgtcatgcc aaccaaaatt cctaacctgc tggtgaacgg ttcttccggt atcgccgtag
     5101 gtatggcaac caacatcccg ccgcacaacc tgacggaagt catcaacggt tgtctggcgt
     5161 atatcgatga tgaagacatc agcattgaag ggctgatgga acacatcccg gggccggact
     5221 tcccgacggc ggcaatcatt aacggtcgtc gcggtattga agaagcttac cgtaccggtc
     5281 gcggcaaggt atatatccgc gcccgtgctg aagtggaagt tgacgccaaa accggacgtg
     5341 aaaccattat cgtccacgaa attccgtatc aggtaaacaa agcgcgcctg atcgagaaga
     5401 ttgcggaact ggtaaaagaa aaacgcgtgg aaggcatcag cgcgctgcgt gacgagtctg
     5461 acaaagacgg tatgcgcatc gtgattgaag tgaaacgcga tgcggtcggt gaagttgtgc
     5521 tcaacaacct ctactcccag acccagttgc aggtttcttt cggtatcaac atggtggcat
     5581 tgcaccatgg tcagccgaag atcatgaacc tgaaagacat catcgcggcg tttgttcgtc
     5641 accgccgtga agtggtgacc cgtcgtacta ttttcgaact gcgtaaagct cgcgatcgtg
     5701 ctcatatcct tgaagcatta gccgtggcgc tggcgaacat tgacccgatc atcgaactga
     5761 tccgtcatgc gccgacgcct gcagaagcga aaactgcgct ggttgctaat ccgtggcagc
     5821 tgggcaacgt tgccgccatg ttggaacgtg ctggcgacga tgctgcgcgt ccggaatggc
     5881 tggagccaga gttcggcgtg cgtgatggtc tgtactacct gaccgaacag caagctcagg
     5941 cgattctgga tctgcgtttg cagaaactga ccggccttga gcacgaaaaa ctgctcgacg
     6001 aatacaaaga gctgctggat cagatcgcgg aactgttgcg tattcttggt agcgccgatc
     6061 gtctgatgga agtgatccgc gaagagctgg agctggttcg tgaacagttc ggtgacaaac
     6121 gtcgtactga aatcaccgcc aacagcgcag acatcaacct ggaagatctg atcacccagg
     6181 aagatgtggt cgtgacgctc tctcaccagg gctacgttaa atatcagccg ctttctgaat
     6241 acgaagcgca gcgtcgtggc gggaaaggta aatctgccgc acgtattaaa gaagaagact
     6301 ttatcgaccg actgctggtg gcgaacacgc acgaccatat tctgtgcttc tccagccgtg
     6361 gtcgcgtcta ttcgatgaaa gtttaccagt tgccggaagc cactcgtggc gcgcgcggtc
     6421 gtccgatcgt caacctgcta ccgctggaac aggacgaacg tatcaccgcg atcctgccgg
     6481 tgaccgagtt tgaagaaggc gtgaaagtct tcatggcgac cgctaacggt actgtgaaga
     6541 aaaccgtcct caccgaattc aaccgtctgc gtaccgccgg taaagtggcg atcaaactgg
     6601 ttgacggcga tgagctgatc ggcgttgatc tgaccagtgg cgaagatgaa gtaatgctgt
     6661 tctccgccga aggtaaagtg gtgcgcttta aagagtcttc tgtccgtgcg atgggctgca
     6721 ataccactgg tgtgcgcggt attcgcttag gtgaaggcga taaagtcgtc tctctgatcg
     6781 tgcctcgtgg cgatggcgca atcctcaccg caacgcaaaa cggttacggt aaacgtactg
     6841 cagtggcgga atacccaacc aagtcgcgtg cgacgaaagg ggttatctcc atcaaggtta
     6901 ccgaacgtaa cggtttagtg gttggcgcgg tgcaggtaga tgactgcgac cagatcatga
     6961 tgatcaccga tgccggtacg ctggtacgta ctcgcgtttc agaaattagc atcgtgggcc
     7021 gtaacaccca gggcgtgatc ctcatccgta ctgcggaaga tgaaaacgta gtgggtctgc
     7081 agcgtgttgc tgaaccggtt gacgaggaag atctggatac catcgacggc agtgccgcgg
     7141 aaggggatga tgaaattgct ccggaagtgg acgttgacga cgagccagaa gaagaataat
     7201 tttacttctt catgccaaaa gggagctatc tcccttgttt gaattgaaaa gtccaggctg
     7261 caaagtctgg gcttttgtcg tattagggcg cggtaaagtt tggctgtgcc cgtaaaaaat
     7321 ggctggctat acacaaggaa tgtggcaatg agtggtgaaa aaaaggcgaa aggctggcgg
     7381 ttctatggtc ttgtaggttt tggcgcaata gcactgcttt ccgctggcgt ctgggcgttg
     7441 caatatgctg gcagtgggcc agaaaaaacg ttgtcgccgc tggtggtgca caacaatctg
     7501 caaatcgatc tcaatgagcc ggacctcttt ctagacagcg actctctgag ccagcttccc
     7561 aaagatctcc tcaccattcc gtttctccac gatgttctga gcgaggattt cgttttctat
     7621 tatcagaatc atgccgatcg tctgggcatt gaaggcagca ttcgccgcat tgtctatgaa
     7681 cacgatctca cgctgaaaga taagctcttt tcgtcactct tagatcagcc cgcgcaggcg
     7741 gcgctatggc acgataaaca aggccatctt tcacattaca tggtgctgat ccagcgcagt
     7801 ggtttaagca aactgctgga gccattgttg tttgccgcta ccagcgacag ccagttaagc
     7861 aaaacggaaa tcagtagcat caagataaat agtgaaacta ttcctgttta tcagttgcgc
     7921 tataacggca ataacgccct gatgttcgcg acatatcagg acaagatgct ggtgttttcc
     7981 agtacggata tgttgtttaa agatgatcag caggataccg aagccacggc gatcgcaagt
     8041 gatttgttga gcggtaaaaa acgctggcaa gccagctttg gcctggaaga gcgtacagcc
     8101 gaaaaaacgc cagtacgcct gcgcatcgtg gccagcgcca ggttgctggg ttttggctac
     8161 cagcggttaa tgccttcttt tgctggcgta cgcttcgaaa tgggtaacga cggctggcac
     8221 agttttgtgg cgttaaatga tgaatccgcc agcgtagatg ccagtttcga ttttacgccg
     8281 gtctggaaca gtatgcctgc cggagccagc ttctgtgtgg cggtgccgta ttcacacggt
     8341 attgccgaag agatgctttc gcacatcagc caggaaaacg acaagctgaa tggggcgtta
     8401 gacggtgccg cggggctgtg ctggtatgaa gactcaaaat tgcaaacccc gctgtttgtc
     8461 ggtcagtttg atggcactgc cgaacaggcg caattgccag ggaaactgtt tacgcaaaat
     8521 attggtgcgc acgaaagcaa agcgccagaa ggtgttttgc cggtaagcca gactcagcag
     8581 ggcgaagcgc aaatctggtg tcgcgaagtg agttcccgat acggtcagta tccgaaagcg
     8641 caggcggcgc aacccgatca attaatgtcg gattattttt tccgtgtgtc gctggcgatg
     8701 caaaacaaaa cgctgctttt ctccctcgat gacacgctgg ttaataacgc cctgcaaaca
     8761 ctgaataaaa cccgcccggc aatggtggat gtaataccca ctgatggcat cgttccgctc
     8821 tatatcaatc cacaaggcgt ggcgaaactg ctgcgtaacg aaacgttgac cagtctgccg
     8881 aagaatctcg aaccggtttt ttataacgcc gcacaaactt tattaatgcc gaagctggac
     8941 gctttatctc aacaaccgcg ttatgtcatg aagctggccc agatggaacc cggtgtcgcc
     9001 tggcagtggc tacccataac ctggcagccg ctatgagaca cgggctgctg gcgctgattt
     9061 gctggctgtg ttgtgttgtt gcccatagcg aaatgctgaa tgtcgaacaa tccaggctgt
     9121 ttcgcgcctg gtttgtacgc attgcacaag aacagctccg ccaggggcca agtccacgct
     9181 ggtatcagca ggattgtgcg ggcctggtgc gatttgcggc gaacgagacg ctgaaagttc
     9241 acgacagtaa atggctaaaa agtaacggtt tatctagcca gtatttgccg ccagagatga
     9301 cgctaacacc tgaacagcgt caactggcgc aaaactggaa tcaggggagc gggaaaaacg
     9361 gcccctacgt g
//
LOCUS       DADMEQ010000134         9356 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.136, whole genome shotgun sequence.
ACCESSION   DADMEQ010000134 DADMEQ010000000
VERSION     DADMEQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.136"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..356
                     /gene="yeiL"
                     /locus_tag="JNZ05_003420"
     CDS             <1..356
                     /gene="yeiL"
                     /locus_tag="JNZ05_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HAY8566521.1"
                     /translation="PLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLMNRLAAFI
                     LLSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGL
                     TLEMDPENKFSGMMQ"
     gene            complement(427..1677)
                     /locus_tag="JNZ05_003421"
     CDS             complement(427..1677)
                     /locus_tag="JNZ05_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY8566522.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFIA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(1771..2709)
                     /locus_tag="JNZ05_003422"
     CDS             complement(1771..2709)
                     /locus_tag="JNZ05_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HAY8566523.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(2697..3638)
                     /locus_tag="JNZ05_003423"
     CDS             complement(2697..3638)
                     /locus_tag="JNZ05_003423"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HAY8566524.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(4062..5753)
                     /gene="fruA"
                     /locus_tag="JNZ05_003424"
     CDS             complement(4062..5753)
                     /gene="fruA"
                     /locus_tag="JNZ05_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HAY8566525.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(5770..6708)
                     /gene="fruK"
                     /locus_tag="JNZ05_003425"
     CDS             complement(5770..6708)
                     /gene="fruK"
                     /locus_tag="JNZ05_003425"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAY8566526.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(6708..7838)
                     /locus_tag="JNZ05_003426"
     CDS             complement(6708..7838)
                     /locus_tag="JNZ05_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HAY8566527.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            8206..>9356
                     /gene="setB"
                     /locus_tag="JNZ05_003427"
     CDS             8206..>9356
                     /gene="setB"
                     /locus_tag="JNZ05_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HAY8566528.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATL"
BASE COUNT         2298 a         2569 c         2192 g         2297 t
ORIGIN      
        1 gtccgctgtt attaaacgac accctatttt tacgaaaact ttgcgtcacc ttaagtcata
       61 aaaattatcg taatattgtt tctttaactc agaatcaatc atttccgtta atgaatcgcc
      121 tggcagcatt tatattactc tcacaagaag gtgatcttta tcacgaaaag catacgcaag
      181 cggcagagta tttaggcgtt tcttatcgac atcttttata tgttctcgcg caattcattc
      241 acgacggttt attaattaaa agcaagaaag gatatctaat taaaaacaga aagcagttgt
      301 caggactgac gctggagatg gacccggaga ataaattctc cgggatgatg cagtaaaaat
      361 tatttgcaat agcgcgattg ccggatgcaa cgcttaatac gttttatccg gtctacaaat
      421 cgagcattac gccagaccaa taaagaaccc ggcaatagtc gcactcatca ggttggaaag
      481 cgttgctgct gccagcgccc gtaaaccaag ctgggcgatt tctggagcac gttttggcga
      541 aatagccgaa aatgcgccaa cgacaacacc gatagaacca aagttagcaa aaccacaaag
      601 cgcaaaggat ataatcgcca cggttttcac ttccagcgtg ccgcccgttt gcaggtatgg
      661 ggagaaactc aggtaagcga tgaattcgtt aatcgccagt ttctgcccaa tcaggctacc
      721 agcaaggttg gcatcactcc agtccacacc catgatccac gccagcggtg ccagcacata
      781 gccaaaaata ctttccagag aggcattggc gaaaccaaac cagccgccaa ttccgccgat
      841 aatgccgttg atcagcgcaa taattgcgac aaacgccatt accaccgtcg ctacaccagc
      901 ggcgattttc agcccggtca tcgcaccgct cgccgccgct tcgataaagc tttttggcgg
      961 cgtttcgctg aacgacagat tttcaaatgt gacctgcgaa ggctcggttg ccgggctaag
     1021 aatacgtgca aacaaaatac cgccggggat cgccatcagc gatgccgcta acaggtagtc
     1081 aattggtacg cccattccgg cataaccaat catcatcgaa ccagcaatgg acgccatccc
     1141 gctacaaatt gcggtaaaca actcgttgcg attcacgcga tcgataaacg gtttaacgat
     1201 cgccgggatc tcattttgcc cgaggaaaat agtggtaacc gcaacaaaag attcgatttt
     1261 gctgatgttg agggctttct ggaaaatgct gccaaggatg cgaatcagca gccccatcac
     1321 gccaatgtag tacagcagac tgatgagcgc agtaacgaaa ataatcgccg gaagtacgcg
     1381 aaaggcgaag ataaaacccg caccgtcaaa caggacatcc attttcggcc caaccagcga
     1441 accaaaaatg aaggcgctac cggcatcact gtaagacatc actttatgaa cgcctaatgc
     1501 cgcctgttct actgcccatt ttcccggtgg gaagtagagc atgatgccac caatagcgat
     1561 ttgcagcagc agtgcggctc caaccgtgcg caaactgatg ctctttttat tcactgacaa
     1621 cagaaatgct attgccagta acaccaccat ccccacaaca cttctcatta tatccataat
     1681 gattttccct tcatgccagt aaacccggcg tcagcgccag gttttggtat gcttgatgag
     1741 tacgggcgac ggctttctgc ccgtcagaaa ttaacccgcg agacgctggt attctttggc
     1801 aatttcactc gccagaatgg cgttgttgaa caccagctgg atattggatt tcaggctgtc
     1861 accgccggtc agctcagcaa cgcgcgccag caggaacggt gtactttctt taccaataac
     1921 gccctgctct tcggcttcgg cgacggcttg atctatggct gcattgatag attcctccgg
     1981 catcgcaaac tgttccggga tcgggttcgc taccacgagg ccaccgttca gcccgctttg
     2041 ccatttcacc gccattgcac gggcaatttc actggcgctg tcgagacgaa tgctgacgtc
     2101 aaacgagctg gtacggcaga aaaacgcagg cagcgcttta gtctgatagc caattaacgg
     2161 cacaccgaag gtttctaaat actcagtggt taatcctaaa tcgagaatag atttcgcccc
     2221 ggcacaaaca acggtgacat tagtatttgc cagttcttgc aaatcggcag aaatatcgaa
     2281 ggtatgttcc gccccgcgat gcacaccacc aattcccccg gtggcaaata ctttaattcc
     2341 ggcaagcgcc gcaataatca tcgttgaagc cacagtggtt gcgccatttt ttccggcggc
     2401 aacaacaaaa ggtaaatcgc gacgactaac tttggtcacg ttatgccctt cacgacccag
     2461 taattcaatt ttttctttgc ttaaacccac tttcatcacg ccgccaataa tggcgatagt
     2521 ggcaggtact gcgccctgtt tacgaatagt ttcttctact tcaattgcgg tctgggcatt
     2581 ttgtgggaac ggcatcccgt gagaaataat ggttgattcc agcgccacaa ccggtttttt
     2641 gttttttaaa gcgtcctgca cttccggaga aatttgtaat aattcagggg aaatttttaa
     2701 ttcagacatt ctgcgttctc cactaacgat ataacgttgg caatcgataa atcggggtta
     2761 ttggtgtatt cacaggagag cgccattgac gaacatccct gtgcgaaacg aacagattcg
     2821 gcaaacggca ttccgtctac ccaacacgaa gcaagtcccg ccatcatggc atcgcccgct
     2881 ccggtaacat taataacatt ggttttgatc ggcgcagacc agccattttc accgctgata
     2941 tcgctgtaat aaacgccgtc gccgcccatg ctcaatacca gtcggttcag gccatgttga
     3001 tggaaccagg cagcaacttt tgccacatcg tcacgccctg acagcgcaat cccactcagg
     3061 gtttccgctt caaggcggtt tggcttcaga gtgtggatct gatttagacg gtcgcgcact
     3121 ttgacacatt tccacgctga aaccggatcg acaaatacgg gtacgttggc ggcattatcc
     3181 agaatccatg ccagtgcctc ttcactgata ttacagtccg cgacaatgac ctttgccctc
     3241 tgaataaatt cacggtgctg tgcgagatat tcagctgtaa tagcgttgct aatattcatg
     3301 tcatttatag caaccagcat ttcaccggta ttatcgagta atgataaata actcgacgta
     3361 ttttctcccg gcacaatcag gcatttatcg acataaacac cagattgatt ggtttgcgtt
     3421 agcagcgatt gaccataaaa atcactgcct acggcgctca gtagccaggc tttgttaccc
     3481 agcaacgcca ggttttgtgc aatattgcgc cctactccac caggcgtaaa ttttatttta
     3541 cctggatttg aatccgcata atttaatgat tcatgtgaat atccggcgac atcaatattc
     3601 gccgaaccta taattacgac ataatccttt tcgcgcatag cacgtcctct ggtaaaaaat
     3661 taccaaccct gtcaatccac aagaatatag atactgcaaa gacggtttaa acatgtgttc
     3721 atatttaaac atgtgctcat agtatgcatg ttttacgtaa agtaaattac caccagagag
     3781 gcaaaatggg cagatttgag caggctcaca aaatgcgatt aaatgcctga attacaaaag
     3841 cgaggaattt gtgacgggat acacagaaca ggaacactaa aaaagggcag aaaataatct
     3901 gcccttgagg aaataacctt agtgtttgat gaacgtttga agtttctact gaaagataaa
     3961 accatttaaa ttcataaaat tacattcaca agctggtcat tctctttgcg ctcatcaaat
     4021 gttacagggc aggaaatttc tgccctgtaa cacacctttt attacgctgc tttcgctact
     4081 gcgtccactt ccggacgttt caggaaggca taggccaaac ccgccaccag cgtaccggca
     4141 ataattgcta ccaggtaacc caataccggc gtaatagcgc cagggatcag cagaacaaac
     4201 agaccaccgt gcggtgccat cagtttcgca ccaatcgcca ttgagattgc gccagtcagc
     4261 gccccaccca cgatacagca cggcagcaca cgcatcggat cacgagcagc aaacggaatt
     4321 gcaccttccg aaatgaagca cagccccaat accagagcgg ctttgccacc ttcctgctgc
     4381 gctttgtcga atttgcgacg cgccaccatt gttgccaggc ccattgccag cggtggcacc
     4441 atacctgccg ccataatcgc cgccatcggg ccataggttt gagtactcag cagacccaca
     4501 ccgaatgcgt acgctgcttt gtttaccgga ccgcccatgt cagtacacat catgccaccg
     4561 aggatcgccc ccagcagaac cgcattcgca gtccccatgg tctgcagcca gtgagtcagc
     4621 ccttcgagaa tgccagcaac tggtttaccg atcaggtaga tcatcgccag accgaccacc
     4681 agactggaaa ttagcggaat gatcaggatc ggtttcagcg cctccatact ctgcggcagt
     4741 ttcagttgcg tactgattaa cttcgcaatg taaccagcca ggaagcccgc aataataccg
     4801 ccaatgaagc cagaaccggt gctgaccgcc agcataccgc caatcagacc cggcgtgagg
     4861 cccggacgat cggcaatgga gaaggcaata taacctgcca gtaccggcac catcagcgca
     4921 aaggctgaac caccaccaat ctgcatcagc gccgctgcca acgtacccgg ctctttaaac
     4981 gcttcgatac caaaagcaaa agaaagcgcg atacacagac caccagcaac gaccatcggc
     5041 agcatgtaag agacgcccgt tagcaagtga cgataagcgc ctgcactctc tttcttacct
     5101 tcagtggtcg ccgtttgagc tttgcccgcc ggttcatacg gcgttgcttc agcaaccgct
     5161 ttatccagtt cctgcgaggt tttcttcagc gccagaccgg tagaggtacg atacatcggt
     5221 ttaccagcaa atttcgccag atccacttcg atatctgccg ccacaatcac cagatccgct
     5281 gcggctactt cctcgggagt gattgcatta cccgcgccaa cagaaccacg ggtttcaact
     5341 ttcacccacc agccacgttt tttcgcttcg gtttcaatgg cttcagccgc cataaaggtg
     5401 tgtgctacgc cagtcgggca agcagtcacc gcaactacgc gtttcggacc gctggcggca
     5461 accggtgctg tcgcagtgac cggcgcagtg taaggtttcg catggccttt ggcttcactc
     5521 aggaacagct cagggtgcgc aactgcccgg gaaatatcac ccagccagac atttttaccg
     5581 ttcagcgcgc tgtcattcgg gatggaatca ccgagaacaa tcgccatttc tgcgtcgttc
     5641 ggattgtcga tgatttccag ttttgctttt cgcgccgccg cgcccagcag ggtcttcgcc
     5701 atataggcgc gtgcctgacc gagattagcg tcaataatca gcagcgtttt cattatgcct
     5761 ctcctgctgt cagttaaaag gttgtaagtc gacgcgcgcc atcattgcgg ccaactgcgg
     5821 acgatcggta atacccacat tgctttgact taccgccagg gctgcaacag ctgtcgccag
     5881 acgcagtgtg tgttcactgg attcacgcat cagcaagcca taaatcaggc caccaaccat
     5941 agaatcccct gcgccaacgg tgcttacgac atcgactgac ggtggtttag cgatccattc
     6001 gccggaggca ttaacccaaa gcgcgccttc ggcacccagt gaaataacaa catgcgcaat
     6061 accttgttca cgtagcgcat gtgcagcttc aatcacatct ttcatttcag gcagtttacg
     6121 gcctgcccag atttccaact cgcggcggtt aggtttcacc agccacggtg ccgctttcaa
     6181 acctgctact aacgcttcac ggctactatc aaagataatg caaggacact gactacgcag
     6241 gcgagtcatc cagtcggtga acgcttccgg gctgacgcct gacggtaagc ttccgctgac
     6301 acagaccata tcgaactgac cgagccagct cagagaatca gtcacaaagc gttcccagtc
     6361 ggcgggggtg acttcaaaac ccgagaagtt gaagtcggtc acttcgccgt ctttttccgt
     6421 cagcttaacg ttaattcggg tgcgcccctg tacaacctgg aaacggttgg caatgcccag
     6481 ctcgctgaac agttgctgaa aaccatcctg attgtcttta cccaggaagc cgccaacggt
     6541 gacatcaatt cccaggtctt ttaatacttt ggccacgttg atgcctttac ccgccgcatg
     6601 cagaccggtg gttttcacca ggttcacttc gccgcgttca atttccgggc agaaaccaac
     6661 aaggtcataa gccggattaa gggtgatcgt agcaacacgt ctgctcatta tgcgccctcc
     6721 ccaagaccag cagcgatagc gtcgccaatg gctttcagcg cctgttcagc atctgcaccc
     6781 tgggcggtaa agcgtaggcg atgacctttc ttaacgccaa gtgccacaac tttcatcaga
     6841 ctacgtccgt ttgccggttt gccggtgcca tcaaggtttg tcacggtaat atcactgtta
     6901 aattgtttaa tggtattgac cagcatggta cctggacgag catgcaggcc gtgttcattg
     6961 cgcaccacaa actccgcgct taacacgtcg tcggtcggcg catcatcgct ggtcagcagc
     7021 gccagcaacg ttgccgcatc cgctttcagc aagcggtcag ctttattgtc gagcaacaaa
     7081 tcagcgagac gcttaagaac cgcgatgggc tgatcgtcat tcatcgccac actcaccagc
     7141 atggctgccg tttcgccgtc cacatcaaaa gcatttgccg cacggcttac cgcaatcgca
     7201 ctacgcagat tgccttcggc gctatcgctc agccagatac cctgtccgag attcagcggt
     7261 tgttcattga tggctttggt gacgaaagtg gcgtcaactg ccctcgcctc tttcagacgc
     7321 gcagcgttca gcgcctgaag agtcagcaga tcgctggcga cgatatccag cgtcagcatt
     7381 tcgttgtcga gcttcagctg ctcactctgc ttttcgccca tcagtaatgc gcgaagttct
     7441 tctgctgttg ttgctgactt cagttgttca gcaacggaat catcgctcag tacgtgggtc
     7501 agctggcgta gcaggcccag atgtttatcc gagctggcag caataccgat tgccacgtac
     7561 gctacctgac cgtcacccca ggtgacgcct tccgggaact gaaatacctg aacgccggtt
     7621 ttcagcacct gatcgcgggt gtcggtagtg ccgtgtggaa tagcaatacc attgccgagg
     7681 aacgttgagg tttgctgttc gcgcgccagc atgccattga cgtagccttc tgctacatta
     7741 ccggcctgca ccagcgccgc agcgacctgg cgaatcgcct cttctttgtc tccggccttt
     7801 tcgcccggat ggatgtcctg tacggataac tggaacatag ttctcctctc ttgctgaatt
     7861 gaaacgattc agcctctatg agaaaaaaag cgccaacctg gcttagggtt aaagacaaga
     7921 tcgcgctgaa acgtttcaag aaagcataat acttctgttt cagcacgcaa ggaaagccgt
     7981 aaagttatga gcaaaagttt gatgtgctgc acaatttttt gtcatttttc ctaattgctg
     8041 atgggaaaat ccgttatcag caatttcatt tcagcagcgt cagcagccaa tcagcaacag
     8101 tgaaatggca ttttgattat tcgtggcgct tttgatttcg ctgtctgttt attttctgac
     8161 aagcagcgta aactccgcgt cttcctcttc cagtgatcga ccagcatgca taactccccc
     8221 gcagtctcca gcgcgaaatc gtttgacctg acctcgacgg cgtttttaat cgttgccttt
     8281 ctcaccggta ttgcgggtgc tctgcaaacc ccgacactca gtattttcct taccgatgaa
     8341 gtacatgccc gtccggcgat ggtgggattc ttctttaccg gcagcgctgt cattgggatt
     8401 ctggtaagtc agtttctcgc tgggcgctct gataagcgcg gcgatcgcaa atcgctgatt
     8461 gtcttttgct gcctgttagg cgtgctggcc tgcacccttt ttgcctggaa tcgcaactac
     8521 tttgttttgc tattcgttgg cgtctttctt agcagctttg gctcgaccgc taacccgcaa
     8581 atgtttgccc ttgcccgtga acatgccgac aaaaccggac gtgaggcggt gatgttcagc
     8641 tcttttttac gcgctcaggt ttcactggca tgggtcattg gcccaccgct ggcttatgcg
     8701 ttagcgatgg gtttcagctt tacggtaatg tatctgagcg cagcggtagc gtttattgtt
     8761 tgcggcgtga tggtgtggct gtttttaccg tcgatgcaaa aagagcttcc gctggcgacc
     8821 ggcacggttg aagcgccgcg ccgtaaccgt cgcgatacgc tactgctgtt tgtcatttgt
     8881 acattgatgt ggggctcgaa cagcctgtac atcatcaaca tgccgctatt tattatcaac
     8941 gaactgcatc ttcccgagaa actggccggt gtgatgatgg ggaccgccgc cgggctggaa
     9001 atcccgacga tgttgattgc cggatatttc gccaaacgtc tgggtaagcg tttcttaatg
     9061 cgcgttgctg ccgtgggtgg cgtctgtttt tacgcaggaa tgctgatggc gcattcacct
     9121 gccattctgt tgggcttgca gctgctaaat gctattttta ttggcattct gggcggcatc
     9181 gggatgctct attttcagga tttgatgccc ggtcaggcag gttcagccac cacgctctat
     9241 accaacacgt cgcgcgtggg ctggatcatc gcaggatcag tggcgggcat cgtcgccgag
     9301 atctggaatt atcacgctgt gttctggttt gcgatggtga tgattatcgc cactct
//
LOCUS       DADMEQ010000135         9240 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.137, whole genome shotgun sequence.
ACCESSION   DADMEQ010000135 DADMEQ010000000
VERSION     DADMEQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.137"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="JNZ05_003428"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="JNZ05_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HAY8566529.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="JNZ05_003429"
     CDS             1828..3147
                     /locus_tag="JNZ05_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HAY8566530.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3512..4657
                     /locus_tag="JNZ05_003430"
     CDS             3512..4657
                     /locus_tag="JNZ05_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY8566531.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4582..5199
                     /locus_tag="JNZ05_003431"
                     /pseudo
     CDS             4582..5199
                     /locus_tag="JNZ05_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5457..5789
                     /gene="ddpX"
                     /locus_tag="JNZ05_003432"
                     /pseudo
     CDS             5457..5789
                     /gene="ddpX"
                     /locus_tag="JNZ05_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5803..6699
                     /locus_tag="JNZ05_003433"
     CDS             5803..6699
                     /locus_tag="JNZ05_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HAY8566532.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6696..7682
                     /locus_tag="JNZ05_003434"
     CDS             6696..7682
                     /locus_tag="JNZ05_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY8566533.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="JNZ05_003435"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="JNZ05_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="JNZ05_003436"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="JNZ05_003436"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HAY8566534.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2254 a         2255 c         2322 g         2409 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acacttagtt aaacagctat aaatccctaa taaaaatgta tgaatatgaa
     3301 aaaccatacg atattcgctg tcaccgaaaa ataattacga tgggctatac tcttctgaag
     3361 aacagtagcg tagattgaaa ctgtcttaag tcgcttattt cttttcagta tatcttcata
     3421 tttcaggaga atatggtgca gttagtcgat ctcgcacgtt gtgtttcgtt ttgattccgt
     3481 ctgatttccc agccttataa gggtgtgaat tatggagatg tattttaaaa gaatgaaaga
     3541 tgagtggacc ggacttgtcg aacaggcaga tcctctcatt cgtgctaaag ccgcagaaat
     3601 tgcccttgcg catgctcatt atctgagtat cgagttttat cgaattgtcc gcatcgaccc
     3661 gcatgccgaa gaattcttga gtaatgaaca agttgagcgg cagttgaaaa gtgcgatgga
     3721 acgctggatt attaacgtgc tttctgccca ggttgacgat gtcgaaaggc taatacaaat
     3781 ccagcatacc gtcgcggaag tgcatgcccg cataggaatt ccggtagaaa ttgtcgagat
     3841 ggggtttcgg gtgctgaaaa aaatcctcta tccggtaatc ttctcttcgg attattccgc
     3901 cgcagaaaaa cttcaggtct accatttctc gattaacagt attgatatcg cgatggaagt
     3961 gatgacccgc gcgtttacct ttagtgacag tagtgcctca aaggaagatg aaaactatcg
     4021 tatcttctcg ttactggaaa acgccgaaga agaaaaagaa cggcaaatag cctcaatact
     4081 ttcatgggaa atagatatta tctataaagt cctgctggat tctgatttag gcagcagttt
     4141 gcctttaagc caggctgatt ttggcctgtg gtttaaccat aaaggtcgac attattttag
     4201 cggcattgcc gaagtgggcc atatctcccg tctgattcag gatttcgacg gtattttcaa
     4261 tcaaaccatg cgtaacacca ggattttgaa taacagaagt ttgcgggtga aatttctatt
     4321 acagataaga aataccgtat cgcagattat taccttgctg cgtgaattgt ttgaagaagt
     4381 atcgcgccac gaagtcggta tggatgtact gacgaaatta cttaaccgcc gtttcctgcc
     4441 gactatcttc aaacgcgaaa ttgcccatgc caaccggacc ggtacaccgc tgtcagtgct
     4501 gattattgac gttgataaat tcaaagagat caacgatacg tggggccata acactggcga
     4561 tgaaattctg cgtaaagtct cattcctctc gcagaagaga ttggtgaaat cgaaaatatt
     4621 gggcgctggg tcatcgcgga agcttgccgt cagttagcag aatggcgtag ccagaatatt
     4681 catatcccgg cgttatccgt gaacttgtcg gcgctgcact ttcgcagtaa tcagctgcct
     4741 aatcaggtgt ctgatgcaat gcaagcctgg ggtattgacg gccaccagct gacggtggaa
     4801 atcacggaaa gcatgatgat ggaacacgat accgaaatct ttaagcgcat tcagatcctg
     4861 cgtgatatgg gcgtgggttt atcggtagat gattttggca cgggcttttc cggattatcc
     4921 cgcttagtca gtcttccggt aacggaaatc aaaattgaca aaagctttgt cgatcgttgt
     4981 ctgacggaaa aacgcatcct ggccttactt gaagccatta ccagcattgg gcaaagcctc
     5041 aatttaaccg tcgtggcgga aggggtcgaa accaaagagc aatttgagat gctacgtaag
     5101 atccactgtc gcgttattca gggatatttc ttttcccgcc cccttcccgc cgaagaaatt
     5161 ccaggctgga tgagcagcgt gttaccgctg aaaatctgac aaattcctct cgcccgcact
     5221 cgcgggtttt cctttaaggt gacactgtca cttaaatact gtgatttcag ctacgattcc
     5281 gggagattcc ttccttaact ttccctcttg caccaaaact gcactacgct gcacaaatgt
     5341 tgatcaaaag ttaaaaaata taactttaag ccagcattct ggcgcttatc ccggcatggc
     5401 atgagatctg cataagcgga aagcgcagca atttttgtct tatacaggaa ccgtttatgt
     5461 cggataccac cgaactggtt gatttagccg tgatcttccc tgatctggag atcgaattga
     5521 aatatgcctg cgttgataac atcacaggta aagctattta tcagcaagcg cgttgtctgc
     5581 tacataaaga tgcgattacc gcgctggcga aaagtatcag tatcgcccag ctgtcaggct
     5641 tacaactggt gatttacgat gcgtatcgcc cacaacaagc acaggcgatg ttgtggcaag
     5701 cctgcccaga cccgcaatat gttgttgatg tgacggtcgg ttctaatcac agccgtggca
     5761 cggcgctcga cctgacgctt cgtgatgacg tggaggtggt gaatgatgct aagcgaggaa
     5821 acgtccgccg tacgcccaca aaaacaaacg cgatttaacg gtgcaaaact ggtatggatg
     5881 ctgaaaggca gtccgctcac cgtgaccggc gcagtcatca ttgtattaat gcgactgatg
     5941 atgatttttt caccgtggct ggcgacgcat gatcccaacg ccattgattt aaccgcccgc
     6001 cttttgccgc cttctgcggc gcactggttt ggcaccgatg aagtgggacg cgatctgttt
     6061 agccgcgtac tggtcggcag tcagcaatca attctcgccg gattagtggt ggtcgccatt
     6121 gcgggtatga ttggttcgct actcggatgt ctatccggtg tgcttggcgg acgcgcagac
     6181 gccatcatca tgcgcatcat ggacattatg ctgtcgattc cttcgttggt actgacaatg
     6241 gcactggctg ccgctctcgg gccgagttta tttaacgcca tgctggcgat tgctattgtg
     6301 cgaattccct tttatgtgcg cctggcgcgg gggcaagcat tagttgtacg ccagtatacc
     6361 tatgttcagg cggcgaaaac ctttggcgcg tctcgttggc atctgatcaa ctggcatatt
     6421 ttacgtaact ccctaccgcc gctgatcgtg caggcatcgc tggatatcgg tagcgcgatt
     6481 ttaatggccg ccacgttggg atttattggc ctgggtgctc aacaaccgag tgctgaatgg
     6541 ggcgcgatgg tagcgaatgg tcgcaactat gtactcgatc agtggtggta ttgcgcattt
     6601 ccgggggcag cgattttgct taccgccgtc gggtttaatc tctttggtga tggtattcgc
     6661 gatctgcttg acccgaaagc aggaggaaag cagtcatgac ccaacccgtt ctggacattc
     6721 aacagctgca tttgagtttc cccggtttta acggtgatgt tcacgcgctc aacaatgtgt
     6781 ccttgcagat taaccgcggc gaaattgtcg gtctggtggg agaatccggc tcaggtaaat
     6841 cagtcaccgc aatgctgatt atgcgtctgc taccgacggg cagttattgc gtacatcggg
     6901 ggcagatttc actgctggga gaagatgttc ttaacgctcg ggaaaaacag cttcgtcagt
     6961 ggcgcggcgc acgagtggcg atgatctttc aggaaccgat gaccgccctt aatccgacac
     7021 gtcgaatagg tcttcagatg atggacgtaa tccgccatca tcaaccaata agtcgtcggg
     7081 aagccagagc taaagcgatt gccctgctgg aagagatgca aatcccggat gccgtggaag
     7141 ttatgtcgcg ctatccgttt gagctttcag gtggtatgcg ccagcgggta atgattgcgc
     7201 tggcattctc ctgcgagccg caattgatta ttgccgacga accgacgacg gcgctggacg
     7261 tcacagtaca gttgcaggta ctgcgtttgc ttaaacataa agcacgcgcc agtggaactg
     7321 cggtactgtt catcagccat gatatggccg tggtgtcgca actgtgcgat agcgtttacg
     7381 tgatgtatgc cggaagcgta attgaaagcg gcgggacggc agacgttatc catcatcccc
     7441 ggcatccgta taccattggt ttgctgcaat gcgcaccaga acatggagta ccacgccagc
     7501 cattacccgc cattccaggg acggtaccaa acctcaccca tttgcctgac ggctgcgctt
     7561 ttcgcgatcg ttgctatgcg gcaggtgcac agtgtgaaaa cgtcccggcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcgcctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggtaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccaggg cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttactgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gagctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgagctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat atatatctca
//
LOCUS       DADMEQ010000136         9161 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.138, whole genome shotgun sequence.
ACCESSION   DADMEQ010000136 DADMEQ010000000
VERSION     DADMEQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.138"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            1..924
                     /locus_tag="JNZ05_003437"
                     /pseudo
     CDS             1..924
                     /locus_tag="JNZ05_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1176..2093
                     /gene="oxyR"
                     /locus_tag="JNZ05_003438"
     CDS             1176..2093
                     /gene="oxyR"
                     /locus_tag="JNZ05_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HAY8566535.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="JNZ05_003439"
     CDS             complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="JNZ05_003439"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HAY8566536.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCKILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3797..4447
                     /gene="fabR"
                     /locus_tag="JNZ05_003440"
     CDS             3797..4447
                     /gene="fabR"
                     /locus_tag="JNZ05_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HAY8566537.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4447..4806
                     /locus_tag="JNZ05_003441"
     CDS             4447..4806
                     /locus_tag="JNZ05_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HAY8566538.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4846..5946)
                     /gene="trmA"
                     /locus_tag="JNZ05_003442"
     CDS             complement(4846..5946)
                     /gene="trmA"
                     /locus_tag="JNZ05_003442"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HAY8566539.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6060..6250
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6315..8159
                     /gene="btuB"
                     /locus_tag="JNZ05_003443"
     CDS             6315..8159
                     /gene="btuB"
                     /locus_tag="JNZ05_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HAY8566540.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNCTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8104..8961
                     /gene="murI"
                     /locus_tag="JNZ05_003444"
     CDS             8104..8961
                     /gene="murI"
                     /locus_tag="JNZ05_003444"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY8566541.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2097 a         2219 c         2468 g         2377 t
ORIGIN      
        1 ctgggtattg gcgaagcgcc atttatgcct gcgggagtaa agtcgatcac cgactgatat
       61 gcacaaaaag aacgcggcac ggcgctgggg atttttaact catctaccgt tatcggtcag
      121 gctatcgcgc ctcctgctct tgtattgatg cagctggcat ggggctggcg gacgatgttc
      181 gttatcatcg gcgtggcagg gattctggtt gggatctgtt ggtacgcgtg gtatcgcaac
      241 cgggcgcagt ttgtcctgac tgacgaagaa cggacgtatc tctccgcgcc ggttaaaccg
      301 cgtccacagc tgcaatttag cgagtggctg gcgctgttta agcatcggac aacctggggg
      361 atgattttag gtttctctgg cgtcaattat accggttggc tctacattgc gtggttaccc
      421 ggttatttgc aagccgaaca aggtttcagt ctggcgaaaa ccggttgggt agcggcgatt
      481 cctttcctcg cggcggcggt cgggatgtgg gttaacggta ttgtggtcga tcggctggcg
      541 aaaaaaggct acgacctggc gaagacgcgt aaaacggcta ttgtctgcgg tttgatgatg
      601 tcggcattag gcacgttgct ggtcgtgcaa tcttcctcgc cagctcaggc ggtagcgttt
      661 atctcaatgg cgctgttctg cgtgcatttc gcgggaacgt ctgcatgggg actggtgcag
      721 gtgatggtgt cagaaacaaa agtggcttcc atcgccggga ttcaaaactt tggcagtttt
      781 gtttttactt cctttgctcc gatcgtaacc ggttgggtag tggataccac acactcgttt
      841 aatctggcgc tggttatcgc ggcctgtgtg acgttcaccg gggcgctgtg ttacttcttt
      901 attgtcaaag atcgcattga gtaatcgtta ctatcaggct ctcttgctgt gggcctgtag
      961 aataaaaaaa agcggatcct ggagatccgc aaaagttcac gttggcttta gttattcgag
     1021 ttgagaaact ctcgaaacgg gcagtgactt caagggttaa aagaggtgcc gctccgtttc
     1081 tgtgagcaat tatcagtcag aatgcttgat agggataatc gttcattgct attctaccta
     1141 tcgccatgaa ctatcgtggc gatggaggat ggataatgaa tattcgtgat cttgagtacc
     1201 tggtggcatt ggctgaacac cgccattttc ggcgtgcggc agattcctgc cacgttagcc
     1261 agccgacgct tagcggccaa attcgtaagc tggaagatga gctgggcgtg atgttgctgg
     1321 agcggaccag ccgtaaagtg ttgttcaccc aggcgggaat gctgctggtg gatcaggcgc
     1381 gtaccgtgct gcgtgaggtg aaagtcctta aagagatggc aagccagcag ggtgagacga
     1441 tgtccggacc gctgcatatt ggtttgattc ctacggttgg accatacctg ctaccgcata
     1501 ttatcccgat gctgcaccag acatttccaa agctggaaat gtatctgcat gaagcacaga
     1561 cccaccagtt actggcgcaa ctggacagcg gcaaactcga ttgcgtgatc ctcgcgctgg
     1621 tgaaagagag cgaagcattc attgaagtgc cgttgtttga tgagccaatg ttgctggcta
     1681 tctatgaaga tcacccgtgg gcgaaccgcg aatgcgtact gatggccgat ctggcagggg
     1741 aaaaactgct gatgctggaa gatggtcact gtttgcgcga tcaggcaatg ggtttctgct
     1801 ttgaagccgg ggcggatgaa gatacacact tccgcgcgac cagcctggaa actctgcgca
     1861 acatggtggc ggcaggtagc gggatcactt tattgccagc gctggctgtg ccgccggagc
     1921 gcaaacgcga tggggttgtt tatctgccat gcattaagcc ggaaccacgc cgcactattg
     1981 gcctggttta tcgtcctggc tcaccgctgc gcagccgcta tgagcagctg gcagaggcca
     2041 tccgcgcaag aatggatggc catttcgata aagtgttaaa acaggcggtt taaaccgttt
     2101 aacgcagcta cccgataggc ttccgccatc gtcgggtagt taaaggtggt gttgacgaag
     2161 tactcaatag tgttgccgcc acctttctgt tccataatcg cctgaccgat atgaataatt
     2221 tcggcagcgc gctcgccaaa gcagtgaata cccagaatct cttttgtttc ccgatggaac
     2281 aaaattttca gcgtgcccac gttcatgccg acgatttgtg cacgtgccag atgtttgaac
     2341 tgggcgcggc ccacttcata cggcactttc atcgcggtca gctgctgttc ggttttgccc
     2401 acagagctga tttccgggat ggtgtaaatg ccggtaggga tatcttcaat cagatgtgcg
     2461 gtggcttcgc cttttaccag cgcctgcgcg gcaatgcgcc cctggtcata ggccgctgat
     2521 gccaggctcg gataaccaat cacgtcgccc accgcgtaaa cgtgcggctg tgcggtctga
     2581 tacatgctgt tgaccttcag ctgtccgcga ctgtcagttt ccagcccaat gttctgtaac
     2641 gccagcgaat cggtattacc ggtacgaccg ttggcataga gcaggcagtc agctttcagt
     2701 tttttacccg acttcagatg catgatcaca ccgtcgtcac agctttcgat cttctcgtac
     2761 tcttcgttgt gacgaatcac tacgccactg ttccagaagt gataggagag agaatctgac
     2821 atctcttgat cgagaaatgc cagcaggcga tcgcgggtgt tgatcagatc cacttttaca
     2881 tccataccgc ggaagatcga cgcatattca cagccgatca ctccagcacc atagataagt
     2941 acatggcgcg gttcgtggtg catgctaaga attgagtcgc tgtcgtaaat gcgtggatgg
     3001 gtgaaatcaa catctgttgg atgatatgga cgagagccgc aggcaataac aaatttttca
     3061 gcggttagtg tttcaacgct gccgtccggg caatccagcg ccaacgtatg ctcgtcaaca
     3121 aagcgagcgt tcccctgcaa tattttacag tgattacgtt cgtaaaatcc ctgacgcatg
     3181 cgcgtttgtt gattaatcac gttatcggca tggttaagga tatcggcaaa agaagagcgg
     3241 agcagtcggg aatggtcgct gtatagtggg ttttgattga attctataat gcggctgacg
     3301 gcgtgacgga gagctttcga cgggatggtg ccccagtggg tgcaaccgcc gccaacattt
     3361 tgataacgct cgataactgc gacgcgcgca ccttgcttaa ccaggcccat tgcagcgcct
     3421 tcgccgccgg ggccggaacc tattactatg gcatcgtaat cgtaggaatg tggcatggta
     3481 gggcttacct gttcttatac ataaaatcaa cataatggta acattctatc gcgggtaagc
     3541 caattgatcc ccgtcattta tctggctata tcctgagcgg cctttgcttt gtctgtttct
     3601 tacttttgcc ctgacgtttt attggatttt tatcgacgat actctccgtt taagcggcag
     3661 gtttccgctg tacgtaaaag aaccggccag taaatatgtt ttattgcgtt accgttcatt
     3721 cacaatactg gagcaatcca gtatgttcat tctctggtat agtgccagca gtacttttgg
     3781 caaggattca gacatcgtga tgggcgtaag agcgcaacaa aaagaaaaaa cccgccgttc
     3841 gctggtggaa gccgcattta gccaattaag tgctgaacgc agcttcgcca gcctgagttt
     3901 gcgtgaagtg gcgcgtgaag cgggcattgc tcccacctct ttttatcggc atttccgcga
     3961 cgtagacgaa ctgggtctga ccatggttga tgagagcggt ttaatgctac gccaactcat
     4021 gcgccaggca cgtcagcgta tcgccaaagg cgggagtgtg atccgtacct cggtctccac
     4081 atttatggag ttcatcggta ataatcctaa cgccttccgg ttattattgc gggaacgctc
     4141 cggcacctcc gctgcgtttc gtgccgccgt tgcgcgtgaa attcagcact tcattgcgga
     4201 acttgcggac tatctggaac tcgaaaacca tatgccgcgt gcgtttactg aagcgcaagc
     4261 cgaagcaatg gtgacaattg tcttcagtgc gggtgccgag gcgttggacg tcggcgtcga
     4321 acaacgtcgg caattagaag agcgactggt actgcaactg cgaatgattt cgaaaggggc
     4381 ttattactgg tatcgccgtg aacaagagaa aaccgcaatt attccgggaa atgtgaagga
     4441 cgagtaatga aacaagcaaa tcaagataga ggtacgctgc tgctggcgtt ggttgctggc
     4501 ttatcgatta atggtacttt cgcagcgctg tttagctcca ttgtgccatt ttctgtattc
     4561 ccgattattt ccctggtgct gacggtttac tgcctgcatc aacgttatct taatcgcacc
     4621 atgtcggtag gcttgccggg tctggcagct gcctgtttta ttctcggcgt actgctgtac
     4681 agcacggtag ttcgtgcgga atatccggat atcggctcta acttcttccc ggcagtactc
     4741 tccgtcatta tggtgttctg gattggcgcg aagatgcgta accgtaagca ggaagttgct
     4801 gagtaatcgg tatcatgccg gatgctggcg catccggcat gggttttact tcgcggtcag
     4861 taatacgccg cactccatat ggtgcgtgta ggggaactga tcaaacagag ccagacgttc
     4921 gaccttgtgc gtctggctta atgtttccag atttttgcat aacgtttccg gattgcagga
     4981 gatatacaga atacgtggat acgcctgcac cattttctcg gtttcactgt ccagaccgct
     5041 gcgcggtggg tcgacaaaaa tggtttcgaa ctgatagctc tttaagtcga tcccttgcag
     5101 gcggttaaac tcgcgcacac cattcatcgc ctgagtaaat tcttctgccg ccatacgaat
     5161 aatttgtacg ttatcaatat ggttagcggc aatgttgtat tgcgcggcag cgactgacgg
     5221 tttggcgatt tcggtggcta atacccgatc aaaattgcgc gccagcgcta atgaaaagtt
     5281 accgttgccg cagtacagct ccagtaaatc gccttttgag cctttggtta cgtccagcgc
     5341 ccattccagc atttgaatat tcatcgccgc gttcggctgg gtaaagctgt tttctacctg
     5401 acgatagatc atctctttcc ctgcgaccgg cagacgctcg tcgatgtaat cttgatccag
     5461 cgcgattttg gttttcgttg cccgaccaat cagatgcaca ttcagattct gcgcgcgcag
     5521 tgcatcgcgc agtgcttccg cttcctgacg ccactcatca tccagctttt tatggtacag
     5581 cagggaaacc accgcctgat tactcagcgt ggtgaggtaa tcaatctgga acaacttgtg
     5641 gcgcagaacg ggattattac gcacacccgc aatcatcgcc gtcatcaact ggttgatgag
     5701 ttcactggcg gcggggaagc tatccacgcg gatgcggctt ttggtttgtt gatcgaaaat
     5761 gatgtgatac aggtcatcgc catcgtgcca gatgcggaac tccgcgcgca tccggtaatg
     5821 actgaccggc gagcgaaaca cttccggaac caggtcagaa aacggtgcca tcatactttg
     5881 caaacgtacc actttttcgg ctaactgcgc ttcatactgt tctgttggaa ggtgttcggg
     5941 ggtcatgatg tatcctgaaa aattaaagta cgcggcgatt gtagggattg ctcatcagat
     6001 gtccagatct tgatgaattc ctatttgtga gctacgtctg gacagtaact tgttacaacc
     6061 tgtagcatcc acttgccggt cctgtgagtt aatagggaat ccagtgcgaa tctagagctg
     6121 acgcgcagcg gtaaggaaag gtgcgatgat tgcgttatgc ggacactgcc atccggtggg
     6181 aagtcatcat ctcttagtat cttagatacc cctccaagcc cgaagacctg ccggccaacg
     6241 tcgcatctgg ttctcatcat cgcgtaatat tgatgaaacc tgcggcatcc ttcttctatt
     6301 gtggatgctt tacaatgatt aaaaaagctt cgttgctgac ggcgtgttcc gtcacggcat
     6361 tttccgcttg ggcacaggat accagcccgg atactctcgt cgttactgct atccgttttg
     6421 aacagccgcg cagcactgtg cttgcaccaa ccaccgttgt gacccgtcag gatatcgacc
     6481 gctggcagtc gacctcggtc aatgatgtgc tgcgccgtct tccgggcgtc gatatcaccc
     6541 aaaacggcgg ttcaggtcag ctctcatcta tttttattcg cggtacaaat gccagtcatg
     6601 tgctggtgtt aattgatggc gtccgcctga atctggcggg ggtgagtggt tctgccgacc
     6661 ttagccagtt ccctattgcg cttgtccagc gtattgaata tatccgtgga ccgcgctccg
     6721 ctgtttatgg ttccgatgca ataggcgggg tggtgaatat catcacgacg cgcgatgaac
     6781 ccggaacgga aatttcagca gggtggggaa gcaatagtta tcagaactat gatgtctcta
     6841 cgcagcaaca actgggggat aaaacacggg taacgctgtt gggcgattat gcccatactc
     6901 atggttatga tgttgttgcc tatggtaata ccggaacgca agcgcagaca gataacgatg
     6961 gttttttaag taaaacgctt tatggggcgc tggagcataa ctgtactgat gcctggagcg
     7021 gctttgttcg cggctatggc tatgataacc gtaccaatta tgacgcgtat tattctcccg
     7081 gttcaccgtt gctcgatacc cgtaaactct atagccaaag ttgggacgcc gggctgcgct
     7141 ataacggcga actgattaaa tcacaactca ttaccagcta tagccatagc aaagattaca
     7201 actacgatcc ccattatggt cgttatgatt cgtcggcgac gctcgatgag atgaagcaat
     7261 acaccgtcca gtgggcaaac aatgtcatcg ttggtcacgg tagtattggt gcgggtgtcg
     7321 actggcagaa acagactacg acgccgggta caggttatgt tgaggatgga tatgatcaac
     7381 gtaataccgg catctatctg accgggctgc aacaagtcgg cgattttacc tttgaaggcg
     7441 cagcacgcag cgacgataac tcacagtttg gtcgtcatgg aacctggcaa accagcgccg
     7501 gttgggaatt catcgaaggt tatcgcttca ttgcttccta cgggacatct tataaggcac
     7561 caaatctggg gcaactgtat ggcttctacg gaaatccgaa tctggacccg gagaaaagca
     7621 aacagtggga aggcgcgttt gaaggcttaa ccgctggggt gaactggcgt atttccggat
     7681 atcgtaacga tgtcagtgac ttgatcgatt atgatgatca caccctgaaa tattacaacg
     7741 aagggaaagc gcggattaag ggcgtcgagg cgacagccaa ttttgatacc ggaccactga
     7801 cgcatactgt gagttatgat tatgtcgatg cgcgcaatgc gattaccgac acgccgttgt
     7861 tacgccgtgc taaacagcag gtgaaatacc agctcgactg gcagttgtat gacttcgact
     7921 ggggtattac ttatcagtat ttaggcactc gctatgataa ggattactca tcttatcctt
     7981 atcaaaccgt taaaatgggc ggtgtgagct tgtgggatct tgcggttgcg tatccggtca
     8041 cctctcacct gacagttcgt ggtaaaatag ccaacctgtt cgacaaagat tatgagacag
     8101 tctatggcta ccaaactgca ggacgggaat acaccttgtc tggcagctac accttctgaa
     8161 ccacgtccca ccgtgctggt gtttgactcc ggcgtcggtg ggttgtcggt ctatgacgag
     8221 atccggcatc tcttaccgga tctccattac atttatgctt tcgataacgt cgctttcccg
     8281 tatggcgaaa aaagcgaagc gtttattgtt gagcgagtgg tggcaattgt caccgcggtg
     8341 caagaacgtt atccccttgc gctggctgtg gtcgcttgca acaccgccag taccgtttca
     8401 cttcctgcat tacgcgaaaa gttcgacttc ccggttgttg gtgtcgtgcc ggcgattaaa
     8461 cctgctgcac gtctgacggc aaatggcatt gtcggattac tggcaacccg cggaacagtt
     8521 aaacgttctt atactcatga gctgatcgcg cgtttcgcta atgaatgcca gatagaaatg
     8581 ctgggctcgg cagagatggt tgagttggct gaagcgaagc tgcatggcga agatgtttct
     8641 ctggatgcac taaaacgtat cctacgcccg tggttaagaa tgaaagagcc gccagatacc
     8701 gttgtattgg gttgcaccca tttccctcta ctacaagaag aactgttaca agtgctgcca
     8761 gagggaaccc ggctggtgga ttctggcgca gcgattgctc gccgaacggc ctggttgtta
     8821 gaacatgaag ccccggatgc aaaatctgcc gatgcgaata ttgccttttg tatggcaatg
     8881 acgccagaag ctgaacaatt attgcccgtt ttacagcgtt acggcttcga aacgctcgaa
     8941 aaactggcag ttttaggctg atttggttga atgttgcgcg gtcggaaaat tattttaaat
     9001 ttcctcttgt caggccggaa taactcccta taatgcgcca ccactgacac ggaacaacgg
     9061 caaacacgcc gacgggtcag cggggttctc ctgagaattc cggcagagaa agcaaaaata
     9121 aatgcttgac tctgtagcgg gaaagcgtat tatgcacacc t
//
LOCUS       DADMEQ010000137         9038 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.139, whole genome shotgun sequence.
ACCESSION   DADMEQ010000137 DADMEQ010000000
VERSION     DADMEQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9038
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.139"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="JNZ05_003445"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="JNZ05_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HAY8566542.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1860)
                     /gene="phoA"
                     /locus_tag="JNZ05_003446"
                     /pseudo
     CDS             complement(446..1860)
                     /gene="phoA"
                     /locus_tag="JNZ05_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
     gene            complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="JNZ05_003447"
     CDS             complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="JNZ05_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HAY8566543.1"
                     /translation="MKNLITELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(2403..2606)
                     /locus_tag="JNZ05_003448"
     CDS             complement(2403..2606)
                     /locus_tag="JNZ05_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566544.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2684..3778
                     /gene="ddlA"
                     /locus_tag="JNZ05_003449"
     CDS             2684..3778
                     /gene="ddlA"
                     /locus_tag="JNZ05_003449"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAY8566545.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASATCMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGELVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITHLIELALERHAADNALKTTM"
     gene            complement(3802..4014)
                     /locus_tag="JNZ05_003450"
     CDS             complement(3802..4014)
                     /locus_tag="JNZ05_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 family protein"
                     /protein_id="HAY8566546.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4274..4582
                     /locus_tag="JNZ05_003451"
     CDS             4274..4582
                     /locus_tag="JNZ05_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2755 family protein"
                     /protein_id="HAY8566547.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="JNZ05_003452"
     CDS             complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="JNZ05_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HAY8566548.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGSYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="JNZ05_003453"
     CDS             complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="JNZ05_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HAY8566549.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQGVTHTLS"
     gene            7320..8477
                     /gene="ampH"
                     /locus_tag="JNZ05_003454"
     CDS             7320..8477
                     /gene="ampH"
                     /locus_tag="JNZ05_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HAY8566550.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8478..8981)
                     /locus_tag="JNZ05_003455"
     CDS             complement(8478..8981)
                     /locus_tag="JNZ05_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8566551.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
BASE COUNT         2238 a         2315 c         2150 g         2335 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagattgt tgcgttgctt gattattaca ttcgcgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cggttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca aggattcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca ccggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt ttttattcag cgcatagtga gtgtattgcc cggtaagcgg
     1561 taaggcatcg atacctttaa aaaagccgcc cgcaccttcg gcataattac gtgcggcagt
     1621 aatttccgaa tcccccatcc catcgccaat cagcaaaata atattttttg caggtttatc
     1681 gctaagagaa tcacgcagag cggcggtctg atcacccgtt aagcggcgag caccgccggg
     1741 tgcagtaata tcgccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc
     1801 ttttgtcaca ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac
     1861 tttattttct ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg
     1921 ccgtgacaac tttatgacag ctattgaaaa gattaacttt ttactgacga ggatgcttca
     1981 ataacttctt tacgtaatcg cgcagcagct ccgtatcgtc gtcaggaatg ctggcatcgg
     2041 gctttacctc gtacagcgcc ccctctacct gatcaatcaa ccgctgttgg tcattttgcg
     2101 ccatattgcg aagcattgca gtgacgataa tctccaaagc ttctacctgc gcacacagtt
     2161 ctttcgactc ttcttctttt tgggcaagct taaataacaa ctcagtaatg agatttttca
     2221 tgtctgtatt tccttatcca aagtatggag aagttaacat tatgtttcac aacttcatag
     2281 tgtctggaag tattattttt atgacaaagg aaatactttg cgcaaatatt ttttgtttga
     2341 ttaccagcga aacgtttctc ttatttaaat aattacccgc acaaaatata ggcttttaat
     2401 tatcaacctt tattataaat aaaagctatg aaatatattc tcttcgttaa cttataatta
     2461 agagaaaaaa actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc
     2521 atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
     2581 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
     2641 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
     2701 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
     2761 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
     2821 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
     2881 tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
     2941 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt
     3001 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
     3061 gtttgtaggt tctgatgttc tggcttcagc aacctgtatg gataaagatg tcaccaaacg
     3121 tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg
     3181 tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
     3241 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
     3301 tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg
     3361 tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga
     3421 gctcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc
     3481 gaaagtggta gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc
     3541 cgttcaggct tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac
     3601 cccagagaac gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag
     3661 tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga tcacacattt
     3721 gattgagctg gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata
     3781 ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat
     3841 aatcaacccc gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg
     3901 ccaacctttt gcttcaaatc ccagtacccc caccacgcca ttaagaatga atataaggcc
     3961 aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc
     4021 tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa
     4081 cattttcgta attgttaata aatttaactt aaggttgcca ttttgtgact cacaacgaag
     4141 attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta
     4201 aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact
     4261 tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg
     4321 caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg
     4381 ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat
     4441 gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt
     4501 cgggctgatc ccgtttttag caggctgcct catttttgca gtggtggcgc tatggctgca
     4561 ctggcgacat cgccgccagt aaaatttacg ttgccagatg cggcctgaaa cgccttatct
     4621 ggcctgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc
     4681 ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca
     4741 ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc
     4801 ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt
     4861 tcgctgtcgc tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg
     4921 ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc
     4981 ttactgactg cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca
     5041 gcaaagcggt atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagaacca
     5101 aaccacaacc cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac
     5161 ggataccctt tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg
     5221 cgcaccggat taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg
     5281 tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt
     5341 ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc
     5401 cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga
     5461 tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagcagac gttttccagc
     5521 gtcggtgcca gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg
     5581 tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg
     5641 ccctttttca acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac
     5701 aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta
     5761 tgggttactc cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg
     5821 tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg
     5881 cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca
     5941 acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg
     6001 cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg
     6061 ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc
     6121 agctctttac ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg
     6181 atccctacca ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc
     6241 agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc
     6301 agcaccggca ggaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc
     6361 tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata
     6421 tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac
     6481 acgtagtgac tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc
     6541 cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt
     6601 agcgcggttt gaatcagatc atagaacggc gcataccagg cgttgacggc gaccccgact
     6661 tccaccaaaa accaagtgac gaagataatc agcgcagtac cgagtattga ccagtattgc
     6721 caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag
     6781 taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc
     6841 ccggaagcgc cggtgatacg tgccacccag tcacccccac cggcttgcca gaagataacg
     6901 gcaatcaatg cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac
     6961 ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc
     7021 atgaccgcta caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct
     7081 tagttcgcca gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt
     7141 acataagcgg attaacacgc gcaggatcac aaatgcaggc agtggtgagc aggaaaaaga
     7201 tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat
     7261 ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt
     7321 tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg
     7381 cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc
     7441 atatttttta cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc
     7501 gcgtctttcg tagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt
     7561 cggtcgtgcg tattgcttcc ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc
     7621 tcgaccaggg gaccgtaaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac
     7681 gcgtgccgac ctacaatggg acgcccatta ccctagtgaa tctggcaacc cataccagcg
     7741 ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc
     7801 gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg
     7861 cggcgtactc taaccttgcg ttcgacctac tggcagatgc actggcgaat gcgtccggca
     7921 agccttatac ccagttgttt gaagagcaga ttacccatcc attggggatg aaagacacca
     7981 cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc
     8041 cgtgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata
     8101 tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag
     8161 atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg
     8221 tccccggcaa agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc
     8281 gtccgaggat tattcagaag acaggcggtg gtggcggttt cattacctat atggcgatga
     8341 tcccgcagaa gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta
     8401 aaaatatgag cgatggcatc aatgatctgg tgaccgagct aagcgggaat aaaccgttgg
     8461 ttatccccgc atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct
     8521 ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa
     8581 cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct
     8641 tcattccagt caatcattga taataatgtt gcgcgggttt gtttggctgg tagatgatat
     8701 cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca
     8761 gccagcccca taatataagg agcctgggta ataccgataa gtacgttttc ttctctacgc
     8821 agagagataa ctccttctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat
     8881 ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg
     8941 agcttaccaa attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta
     9001 atgcccgtca aatccttgac gggcattatt tagattaa
//
LOCUS       DADMEQ010000138         9011 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.140, whole genome shotgun sequence.
ACCESSION   DADMEQ010000138 DADMEQ010000000
VERSION     DADMEQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.140"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(96..1472)
                     /gene="trkA"
                     /locus_tag="JNZ05_003456"
     CDS             complement(96..1472)
                     /gene="trkA"
                     /locus_tag="JNZ05_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HAY8566552.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1494..2783)
                     /gene="rsmB"
                     /locus_tag="JNZ05_003457"
     CDS             complement(1494..2783)
                     /gene="rsmB"
                     /locus_tag="JNZ05_003457"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAY8566553.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2829..3776)
                     /gene="fmt"
                     /locus_tag="JNZ05_003458"
     CDS             complement(2829..3776)
                     /gene="fmt"
                     /locus_tag="JNZ05_003458"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAY8566554.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3791..4300)
                     /gene="def"
                     /locus_tag="JNZ05_003459"
     CDS             complement(3791..4300)
                     /gene="def"
                     /locus_tag="JNZ05_003459"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAY8566555.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4448..5572
                     /gene="dprA"
                     /locus_tag="JNZ05_003460"
     CDS             4448..5572
                     /gene="dprA"
                     /locus_tag="JNZ05_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAY8566556.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNIIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5544..6017
                     /locus_tag="JNZ05_003461"
     CDS             5544..6017
                     /locus_tag="JNZ05_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HAY8566557.1"
                     /translation="MFDVLMYLFETYIHTEAKLRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6046..6588
                     /locus_tag="JNZ05_003462"
     CDS             6046..6588
                     /locus_tag="JNZ05_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAY8566558.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6593..7165
                     /gene="tsaC"
                     /locus_tag="JNZ05_003463"
     CDS             6593..7165
                     /gene="tsaC"
                     /locus_tag="JNZ05_003463"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HAY8566559.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7170..7988
                     /gene="aroE"
                     /locus_tag="JNZ05_003464"
     CDS             7170..7988
                     /gene="aroE"
                     /locus_tag="JNZ05_003464"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAY8566560.1"
                     /translation="METYAVFGNPIAHSKSPLIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7985..8242
                     /locus_tag="JNZ05_003465"
     CDS             7985..8242
                     /locus_tag="JNZ05_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HAY8566561.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8218..8772)
                     /locus_tag="JNZ05_003466"
     CDS             complement(8218..8772)
                     /locus_tag="JNZ05_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAY8566562.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2258 a         2188 c         2246 g         2319 t
ORIGIN      
        1 taacaaatat taagccattt tcctgccagg aatgaattta attaatcctt gataatcaaa
       61 gggaaataat aaggcgtcat tagacgcctt attaattaca agaagaaagg gcttggctgg
      121 aagagtcttt cgacgtcggt aataaacttt ttatctgtga ggaacataat tacgtgatcg
      181 ccttgctcaa tgcgcagatt gtcattggca atcatcacgt cgtttccacg taccaccgct
      241 ccaataatcg ttcctggcgg tagcttgatt tcgtcaatga ctctgccgac aacgcgtgag
      301 gtgctttcat caccgtgagc aacggcttca atagcttctg ctacgccgcg gcgcaatgag
      361 gaaacaccaa caatatctgc ttttcgcaca tggctaagca acgcagaaat agttgcttgt
      421 tgtggtgaaa ttgcaatatc gatcacgctc ccctgaacca gatccacata agcgcgacgc
      481 tggatcaata ccatcacctt tttcgcaccc atacgtttgg caagcatggc ggacatgata
      541 ttggcctcgt catcgttggt gacggcaata aacagatcaa cttgatcgat atgttcttcg
      601 gccagcagtt cttgatccga cgcatcacca aaaaagacga tcgtattctg caacttttcc
      661 gccagttcgg cagcgcgctg ctgattacgt tcgatgagtt taacgctgta atctttttcc
      721 agacgacgcg ccagccctgc accgatatta ccgccaccaa ccagcatgat ccgtttatac
      781 ggtttttcca gtcgctgtaa ttcactcatc accgcgcgga tatgctgtga agcggcaata
      841 aagaacactt catcaccagc ttcaacaatg gtcgaacctt gtggacgaat ggggcgatcg
      901 tggcggaaaa ttgctgccac acgagtatcg atatgtggca tatgttcgcg catggtcgaa
      961 agtgcattac caatcagcgg gccgccataa taggctttaa ctacagcgag gctgacttta
     1021 ccctcagcga agttcaccac ctgcaatgcg cctggatact caatcagtcg gtaaatatta
     1081 tcgataacca actgctctgg tgcgatcaga tgatcaatcg gtacagcatc tgaatgaaat
     1141 agcttatcgg catcgcgtac gtagtctggt gagcggatgc gagcgatgcg attaggggtg
     1201 ttgaaaagtg agtaggctac ctggcaggca accatattgg tttcatctga actggttaca
     1261 gcaaccagca tatcggcgtc gtcggcacct gcctcccgca atacgcgtgg atgagagcca
     1321 tgcccctgca cgactcgcag gtcaaattta tcctgcaagg tccgcagacg ctcaccgttg
     1381 gtatcgacaa cagtaatatc gttgttctcg ccaaccaggt tttccgccag tgtgccgcca
     1441 acctggccgg cacccagaat gataattttc atcagtcgcg acccgttatc tcatcacttt
     1501 ttgattagct tagcgtaaaa gaagccgtcg ccctcttcgg caccaggctg attttgttta
     1561 cccggttgct ctggtgttcc tgtttcgcaa agttcggcat cagcggtacg ttgcaaaaag
     1621 gctttaatct gcaggctatt ctcttccggt aacaccgaac aggtggcata gaccagggtt
     1681 ccaccggatt ttaaatgcgg ccaaatggcg tcgagaattt cagactgcaa ttgcgcgagt
     1741 tccgggatat cgcgatcgcg acgtaaccat ttaatatctg gatggcgacg aatcaccccc
     1801 gttgctgaac aaggcgcatc taataaaatg cgatcaaact gttgctcgcc acaccattgg
     1861 gaagggtaac ggccatcacc ttgtttcacg gttgccttca taccaaggcg ttttaaattg
     1921 tcgtaaacgc gagagaggcg ctgttcgtca atatcaaccg caacaacctg cgcttctggt
     1981 gccacctcaa ggatatgcgt tgttttaccg ccgggggcgg cacaaagatc caagatgtgt
     2041 tcaccgtttt gtggcgcaag ccaggtcatg caaccttgtg ctgatgcatc ctgaacggta
     2101 acccatccgt cttcaaaacc aggtagcgca tgaacaggtg caggtgtttc cagccgtaca
     2161 gcatcagggt aatccgcatg cgggaaacct ttcattcctg cttcatccag caatgcaagc
     2221 cagctgtcgc gggaatgatg cgtacgatta atacgcagcc acattggcgg acgctggtta
     2281 ttggcttcgg cgatggattg ccactgctct ggatacgctt tttgcagacg cttcagcaac
     2341 caggaaggat gcagataacg tgcatcacgg gtattaaact cggctaataa ctcttcttgc
     2401 cgacgctgga actggcgtaa tacaccgtta atcaaccctt taagttgcgg acgcttaatt
     2461 gcgatagcgc cttcaaccgt ttcagccagc gcagcatgag gtggaatgcg ggtataaagc
     2521 agttgataca aaccaaccat aatcaggtaa tgcacagtgc gctgtttgcc ggtcatcgga
     2581 cgggccatta acttattaat cagccagtct aactgcgaaa gcgtacgcag tacgccaaag
     2641 cacaactctt gaagaagtgc tttgtcttta tcggaaactt tttgctggag cggtggcaga
     2701 atgttgctta atgattgccc ttgctcgacg acttgttcaa cggcctgggc cgccatgcta
     2761 cgtaaattac gttgtttttt cataagtata aaaatgctcg gcaagaccgg gcttagaaga
     2821 gtggactatc agaccagacg gttgcccgga acaaaccatt cccgacgaga gttcaggagg
     2881 tcttgcgcgc tcatcgcttt cttacccgca ggttgtaacg agagcaggtt caggatgcca
     2941 tcaccagtcg caacctgaat gccttgtttg ttggcttcaa ggatcgttcc tggtgcagcg
     3001 ttggttgccg tatcaatgac cgacgctttc cagactttaa ccggctgtcc ttcaatttcc
     3061 agccagctca ttggccatgg gttgaaagcg cgaatgcagc gttcaagctg tgctgccgaa
     3121 agtgaccagt caatacgcgc ttcttcttta ctcaacttct cggcgtaagt gacaagagtt
     3181 tcgtcctgaa cttctggttt cgccgtgccg tctgccaatt gtttcaacgt ggtgataagc
     3241 ccttgtgggc caagctctgc cagcttgtcg tacagcgtac cactggtatc ttctgcagta
     3301 atcgggcagg agagcttata gagcatgtca ccggtgtcta aaccaacatc catttgcata
     3361 atggttacac cagtttctgc atcacccgcc catagtgagc gttggattgg tgcagcaccg
     3421 cgccagcgtg gcagcagtga accatgaacg ttgatacagc caagacgcgg catctccagc
     3481 acagctttag gcagaattaa accataggcg acgacgacca taacatcagc ctgcaaatcg
     3541 gcgaccagtt gctggttttc ttgtggacgc agggaaacag gttgaaaaac gggcagacct
     3601 tcttcctcag ccagaacttt aatcgggctg ggcatcagtt ttttaccgcg tcctgccggt
     3661 cggtctggct gggtgaacac gccaacgacg ttatgaccag aagacaacag cgcgtcgaga
     3721 tgacgcgctg caaagtcagg tgtacccgca aaaataatac gtagtgattc tgacacgtta
     3781 gttcttatcc ttaagcccgg gctttcagac gatccagttt ttcaactttc tgacgaattc
     3841 gttgttgttt cagcggtgac agataatcca taaacagttt gccaaccagg tgatccatct
     3901 catgctgaat acagatagct aacagaccct ctgcttccag ttcaaatggt ttaccgtcgc
     3961 ggtcaagggc gcgaatttta actttctctg cgcgcggcac taaagcacgt tgttcaggga
     4021 tcgacaggca accttcttca atgcctgttt cgccgctttt ttctaaaagc tccggattga
     4081 ttaacaccag tcgttcgtca cggttttccg aaacatcaat aacaatgata cgttgatgga
     4141 tatcaacctg ggttgccgct aggccaatac cttcttctgc gtacatcgtc tcgaacatat
     4201 catcgacgat acgctgaatt tctgcattca cttcttctac cggtttagca actttgcgaa
     4261 gccgctcgtc cggaatatgt aacacttgca aaactgacat aaatctccag agatgtgttc
     4321 aggagttaga aagattattt cttctattct agacaaatcc ccctctgatt gacagcatca
     4381 cttgattgac agcatcactg accaatcgca aagattgcta aggcttgctt atgacaggga
     4441 gataaggatg gtcgatacag aaatttggct gcgtttaatg agcatcagca gcttgtacgg
     4501 cgatgatatg gtccgtatcg ctcactggct ggcaaaacag tcgcatattg atgcggttgt
     4561 attgcagcaa acagggctta cattgcggca ggcacaacgc tttctttcat ttccacgaaa
     4621 gagtatcgaa agctcacttt gttggttgga gcaacccaac catcatttaa tccctgcgga
     4681 cagcgaattt tatcctcctc aacttcaggc gacgacagat taccccggcg cactgtttgt
     4741 tgaaggagaa ctgcacgcgc tgcattcatt tcagcttgcc gtagtgggga gtcgggcgca
     4801 ttcatggtat ggcgagcgat gggggcgatt attttgcgaa actctggcga cgtgtggagt
     4861 gacaattacg agtggactgg cgcgtggaat cgatggtgtg gtgcataaag cggccttaca
     4921 ggtaaatggc gtcagcattg ctgtattggg gaatggactt aatatcattc atccgcgtcg
     4981 ccatgcccga ctggctgcca gtctgtttga acagggtggc gctctcgtct cggaatttcc
     5041 cctcgatgtt ccaccccttg cttacaattt cccacgaaga aatcgcatta tcagtggtct
     5101 aagtaaaggt gtactggtgg tggaagcggc tttgcgcagt ggttcgctgg tgacagcacg
     5161 ttgtgcgctt gagcaggggc gtgaagtttt tgccttgcca ggtccaatag ggaatccggg
     5221 aagcgaaggg cctcactggt taataaaaca aggtgcgatt cttgtgacgg aaccggaaga
     5281 aattctggaa aacttgcaat ttggattgca ctggttgcca gacgcccctg aaaattcatt
     5341 ttattcacca gatcagcaag acgtggcatt gccatttcct gagctcctgg ctaacgtagg
     5401 agatgaggta acacctgttg acgtcgtcgc tgaacgtgcc ggccaacctg tgccagaggt
     5461 agttactcaa ctactcgaac tggagttagc aggatggatc gcagctgtac ccggcggcta
     5521 tgtccgattg aggagggcat gccatgttcg acgtactaat gtatttgttt gaaacctata
     5581 ttcacactga agctaagttg cgtgtggatc aagacaaact tgaacaggat cttaccgacg
     5641 caggttttga gcgagaagat atctacaatg ccctgctatg gctggaaaaa cttgctgatt
     5701 atcaggaagg gttggcagaa ccgatgcaac tggcctcgga tcctctctcc atgcgtattt
     5761 atacaccgga agagtgtgaa cgactggatg ccagctgccg tgggtttctg cttttccttg
     5821 agcagattca ggtgctcaac cttgaaactc gtgaaatggt gatagagcga gtgctcgcgc
     5881 tggataacgc agagttcgaa ctggatgatc tgaaatgggt gatcctgatg gtgttgttca
     5941 acatcccggg ctgcgaaaat gcgtaccagc aaatggaaga attactcttt gaagtgaatg
     6001 aaggtatgct gcattaatca tttctttaat tcagcataag ttgttatggc aaaatcagca
     6061 ctgttcacgg tgcgtaataa tgagtcctgc ccaaagtgcg gggctgaact ggttattcga
     6121 tccgggaaac acggtccgtt tcttggatgc tcacagtatc cggcgtgtga ctacgtccgt
     6181 cctctgaaat cttcagcgga tggacatatt gtcaaagttc tggaggggca ggtttgcccc
     6241 gtatgtggcg caaatctggt attacgccag ggacgctttg gtatgtttat tggttgcagt
     6301 aactaccctg aatgcgaaca taccgaactt atcgataaac cggacgaaac agcaattaca
     6361 tgcccccaat gtcggacggg ccatctggtc cagcgccgct cccgttatgg caaaacattt
     6421 cactcctgtg atcgctaccc ggagtgtcaa tttgccatta acttcaaacc aatagctgga
     6481 gaatgccctg agtgtcatta tccgctactc atcgaaaaga aaaccgcgca gggtgtaaaa
     6541 cacttttgtg ccagtaaaca atgtggaaag ccggtttcgg cggaataata acgtgaataa
     6601 taacctgcaa ggagacgcta tcgcagctgc gatagatgtc ctcaatgaaa aacgtgtcat
     6661 cgcctatcca acggaagccg ttttcggtgt cgggtgcgat cctgatagcg aaacagcagt
     6721 gatgagactg ttggagttaa aacagcgtcc ggttgataag gggctgattt taatcgcggc
     6781 aaattacgag cagcttaaac cctatattga tgacaccatg ttgactgacg tgcagcgtga
     6841 aaccattttt tcctgctggc caggtcctgt cacctttgtc tttcccgcgc ctgcgacaac
     6901 accgcgctgg ttgacgggcc gctttgattc gcttgctgta cgagtcaccg accatccgtt
     6961 ggtggttgct ttgtgccagg cttatggtaa accgctggtt tctaccagtg ccaacttgag
     7021 tggattgcca ccttgtcgaa cagtagacga agttcgcgca caatttggcg cggcgttccc
     7081 ggttgtgcct ggtgaaacgg gggggcgttt aaatccttca gaaatccgcg atgccctgac
     7141 gggtgaactg tttcgacagg ggtaacataa tggaaaccta tgctgttttt ggtaatccga
     7201 tagcccacag caaatcgcca ctcattcatc agcaatttgc tcagcaactg aatattgaac
     7261 atccctatgg gcgcgtgttg gcacccatca atgatttcat caacacactg aacgctttct
     7321 ttcgtgctgg tggtaaaggt gcgaatgtga cggtgccttt taaagaagag gcttttgcca
     7381 gagcggatga gcttactgaa cgggcagcgt tggctggtgc agttaatacc ctcaagcggt
     7441 tagaagatgg acgcctgctg ggtgacaata ccgatggtgt aggcttgtta agcgatctgg
     7501 aacgtctgtc ttttatccgc cctggtttac gtattctgct tatcggcgct ggtggagcat
     7561 ctcgcggcgt actactgcca cttctttccc tggactgtgc ggtgacaatc actaatcgga
     7621 cggtatcccg cgcggaagag ttggctaaat tgtttgcgca cactggcagt attcaggcgt
     7681 tgggtatgga cgaactggaa ggtcatgagt ttgatctcat tattaatgca acatccagtg
     7741 gcatcagtgg tgatattccg gcgatcccgt catcgctcat tcatccaggc atttattgct
     7801 atgacatgtt ctatcagaaa ggaaaaactc cttttctggc atggtgtgag cagcgaggct
     7861 caaagcgtaa tgctgatggt ttaggaatgc tggtggcaca ggcggctcat gcctttcttc
     7921 tctggcacgg tgttctgcct gacgtagaac cagttataaa gcaattgcag gaggaattgt
     7981 ccgcgtgaat caggccatcc agtttccgga cagggaagag tgggacgaga ataaaaaatg
     8041 tgtatgtttt cccgctctcg tgaatggtat gcaactgaca tgcgcgatct ctggcgagag
     8101 tctggcgtat cgctttactg gagatacgcc agaacagtgg ttagcgagtt ttcgtcagca
     8161 tcgctgggac ctggaagaag aagcggaaaa cttaattcag gaacaaagtg aagatgatca
     8221 aggctgggtc tggttaccct gatccagata ttcgtccttc catttcacgt aattattcgc
     8281 ggaatagcgt aacccagcct tctcttcatc acttaacggg cggatctgtt tgacggggct
     8341 accgagatac agatatccgc tctccagccg tttattttgt gggaccagac tacccgcacc
     8401 aatcatcaca tcatcttcta ctattgcgcc atcaagtaaa attgagccca tcccaaccaa
     8461 aactcgattg ccaatggtgc agccgtggag catcaccttg tgaccaacag tgacatcttc
     8521 gccaatggtt aatgggttgc catctgggtt gtacgaggat ttatgagtga catgcaacat
     8581 actgccatcc tggatattgg tgcgtgctcc gatctgtaca taatgtacat ctccacgaat
     8641 cacaacgagc ggccagatcc ccacatcatc agccagacga acgtcaccaa tcacgacact
     8701 gctatcgtcg atcattacgc gctgaccgat ttgtggaaaa agatcgcggt atgggcgtaa
     8761 aacatcagac atacttacct cagcaataaa tgatttacta atgactttgg gggcattatt
     8821 ggccttgtgc aagtctttta gtatgcaaaa aagcaccgtt ttgtgtgcga ttgcagcaaa
     8881 aagggtgaaa aaacaacaaa cagaaaaaaa gatcaaaaaa atacttgtgc aaaatattgg
     8941 gaaccctata atgcgcctcc gttgagacga caacgtgaaa cacttcacag gatggtcgga
     9001 acaacgaaga g
//
LOCUS       DADMEQ010000139         8993 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.141, whole genome shotgun sequence.
ACCESSION   DADMEQ010000139 DADMEQ010000000
VERSION     DADMEQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.141"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="JNZ05_003467"
     CDS             complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="JNZ05_003467"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HAY8566563.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKLWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="JNZ05_003468"
     CDS             complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="JNZ05_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HAY8566564.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4606..5763
                     /gene="mdoC"
                     /locus_tag="JNZ05_003469"
     CDS             4606..5763
                     /gene="mdoC"
                     /locus_tag="JNZ05_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HAY8566565.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5771..7252)
                     /locus_tag="JNZ05_003470"
                     /pseudo
     CDS             complement(5771..7252)
                     /locus_tag="JNZ05_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
     gene            complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="JNZ05_003471"
     CDS             complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="JNZ05_003471"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HAY8566566.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7822..8133)
                     /locus_tag="JNZ05_003472"
                     /pseudo
     CDS             complement(7822..8133)
                     /locus_tag="JNZ05_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            complement(8254..8586)
                     /gene="csgC"
                     /locus_tag="JNZ05_003473"
     CDS             complement(8254..8586)
                     /gene="csgC"
                     /locus_tag="JNZ05_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HAY8566567.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQFCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8645..>8993)
                     /gene="csgA"
                     /locus_tag="JNZ05_003474"
     CDS             complement(8645..>8993)
                     /gene="csgA"
                     /locus_tag="JNZ05_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HAY8566568.1"
                     /translation="NNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGAD
                     VGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGATVDQTASNSSVNVT
                     QVGFGNNATAHQY"
BASE COUNT         2252 a         2326 c         2133 g         2282 t
ORIGIN      
        1 aacgccggta tttttattgg ctcgttgttt ttgccggatg tggcgtgaac gccttatccg
       61 acctacaaaa tcttgcaaaa tcaataaatt gcaggaacga tgtaggcctg ataagcgtag
      121 cgcatcaggc aactacgttt ttattgcgaa gccgcatccg gtttacgcaa tgccagtgga
      181 ttgagcttta tcccttcgta ataactcacc catgaagaat atctttcagg ggaattccag
      241 acgcggaaat gcagacgggc catcgtcacc ggatcgctta gcagcaccag gcgacgatcg
      301 cgattcagct tctctggcgt ctcgttaagc gcctgttcaa cgtggcggtc acgggcgatt
      361 tccagcacct tactggcgcg gtgacgcgct gtcgccattg cggttgccag agcgttaaat
      421 gacgggttaa acactgcgtg cataaagcca tcgtcaaggg agcgttggcg attcatctca
      481 aggaaccgat cggtatcaac cagcacctgc ggcggtgaat actcttccgg gatcaggaac
      541 agtttccaga gtttggtacg cagaccaacg gtggcacggc tggaaatcac cgaaacaaac
      601 ggtgacagga tcaacgagaa gacaatcggt gccagccaga acaggaaacg cagatccagc
      661 cacgccatcc caacagccca cactaacccc aacagcagct gtgagccgtg gcgtttgaac
      721 gcttcgcccc atgaagtgga gtcatcatca cgctgcggtg aattccacac cacttcccag
      781 ccaaggaacg cgctgacaac gaagaccgta tggaacagca tgcgtaccgg agccagcagc
      841 acggaaaaca gcacttccag cagcaacgat aatgtaacgc gccagaagcc gccgtattct
      901 ttcgttcctt tgcaccagat aagcaaaatg ctcaataact tcggcaggaa caacagcacc
      961 atggtcgaag caaaaagtgc aatcgccagc tcaggacgcc actgcggcca caccgggaac
     1021 aactgccgtg gttgcaggaa gtattgcggt tcggtcaacg catgcactac ctgcaatgca
     1081 gtagagagcg cgaggaacat aaaccacagc ggagcggaga gataagacat cacgcccgtc
     1141 aggaacaccg cacggtgaac cgggtgcata cccttaacca ggaacagacg gaagttcatc
     1201 aggttaccgt ggcaccagcg gcggtcacgt tttagctcat caagcaagtt aggcggcaat
     1261 tcttcataag aacccgggag atcgtaagca atccagactc cccaacctgc acggcgcatc
     1321 aacgccgctt ctacgaagtc atgtgacagg attgaaccgg caaaggaacc ttcgcccggc
     1381 agcggagcca gtgcgcagtg ctcgataaat ggtttcacgc ggataatcgc gttatgtccc
     1441 cagtagtgcg actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag tggcccatac
     1501 acgcgggtcg cgaactgctg acagcgcgca tacagcgtat ccataccgga cgctttcggc
     1561 gacgactgaa tgatcccggc gttcgggttg gcttccatca agcgcaccag gccgcataaa
     1621 caatcaccgg tcattaccga gtcagcatcc agcaccacca tgtagctgta ctggctgccc
     1681 cagcgacggc agaagtcatc gatattaccg cttttacgct tcacgcggcg acggcggcgg
     1741 cgatagaaaa tctgaccttc gccaccgact tcagcgataa gctccatcca ggctttttgc
     1801 tctgcgacgc agatatccgg gttataactg tcactcagaa tgtagacatc aaagtgtttg
     1861 gcattcccgg tggcttttac tgattcccac gttgcacgca agccagcaaa aacacggttc
     1921 acgtcttcgt tacagatagg catgatcaac gccgtgcgat gctccgggtt taatggttca
     1981 tcgccaactg ttgacgcaga tatactgtat ttatcgcgac caataagcag ttgcaggaag
     2041 cccatcaacg ccgtccagaa tccggcggac acccaacaga acagtaccgc aaaaaggatc
     2101 aggataccgg tttgcagcat ataaggcaga agctgcataa aggaaaccca caaatcctga
     2161 ccaaccatat ccataggatt aatcagcgcc caaccctgat aaggaagaat ggtcttcata
     2221 taccaggtcg cgacaacagt ttgcgcgagc gtcaggatca acagaatgta acggcggatg
     2281 gtaccaacgg tacgccactt ttgctcgctc tcctgctctt ctttggtcaa acgagccaga
     2341 tagcgcggcg tgacatcgcg tccacgcagg cgatcccaga aacggcctac cgggttggta
     2401 cgccacgggt cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt aagctgatcg
     2461 cgcccttcgt cgtctttaat taactgtcca tcagcaagtg aatctggcca ggcttgttcc
     2521 agacgcgcct ttaccgagcc ttgcggggaa tcatcctccc gcgcccaggt gcggtgttcg
     2581 gcatccagcg cctgatgaac ggcgcggata tcagtcttcg gcaatgctgc tttctcgctt
     2641 gcggcgatgg gcattgcgtc aatgtactca gttgtcttat tcattggcag gtaactggta
     2701 gctccaggtt tcactcaacg tctgatcggc attcaccagc gcagcacgca tttcagtggt
     2761 tttcttggca tctttcactt tcacacgcat caccagacgc cagcctttgg taaccgggtt
     2821 atagcgcacc gtgctttcaa ctatctcacc attatcacca atgctggttt gcgctgtgac
     2881 cggggtatcc tctggcagtt ttttcatctc tgcgccggta aaatcgacca caaaggcgat
     2941 agtaccgtca ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg aacgacgcgt
     3001 ttgttgcacc catgcgttat ctggcgcatg cagtttgtct tcatcacggc tgaaggtgat
     3061 ggtgtattta aagttcatct ctttacccgg ctccggcagc tgatccggcg tccagtaagc
     3121 gacgatgtta tcgttggttt catcgttggt tggaatttcc accagctcaa cgctgccttt
     3181 gccccactcc cctttcggag tcacccatgc gcttggacgg agatcgtaac gatcatcgag
     3241 atcttcaaag cgggagaaat cacgacctcg ctgcaacaga ccaaagcctc gcgggttttc
     3301 catggagaag ctgctgaccg ctaaatgttt cgggttattc aacggacgcc agatccattc
     3361 gccattacca gcatggatag acagaccgtt agagtcgtgc aactccggac gatagttatt
     3421 tgcaggcgac ggttggttcg gcccaaacag gaacatactg gttaacggtg caacccccag
     3481 tttgccgact ttatcgcgca gatagatttt cgactgcaca tccacaaccg tgtcacgccc
     3541 tggcataacc acgaatttgt aagcacctgt cgcacgcggc gagtcaagca atgcataaat
     3601 ggttaaacgt ttatcagtcg gttttggacg ctcgatccag aactctttga agcgtggaaa
     3661 ttcttcaccc gatggcaaag cggtatcaat tgctaggccg cgggcagaaa ggccataaac
     3721 ctgacctgca ccaatcacgc ggaaatagct ggccccgagc atgctgacga tttcatcgtt
     3781 tttatctttg ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa ggtcttttac
     3841 cgtgtctttg tcatgctgaa catcgccgaa agtgaaataa tccgggctgt atttgattcg
     3901 tttgactgcg gtggcagtca cttcatttat tttgaccggg gtatcgaagt acataccctg
     3961 atggtagaac tcgagtttga atggggtctt cagattgttc cagtacgctt tgtcatgatt
     4021 aaactggatc tgctgatagt ccgcgtattt catgtcgcgg aaaacggagg gcaagttgct
     4081 tttaggcgcc tcatagcctt tcccggctaa ggattgagct tgctttgcga catcatcaat
     4141 actgaaagcc cagcttgaag atgtatacag ggttaacatt actgcagcac tcaaccaacg
     4201 cattttcatc atttgtagtt tatgtttcat aattagtaag cacttccccc tttgtgtgct
     4261 tatatcgatc cgatctattt taatggataa ccagccattc cgacaactga atcccggtat
     4321 cgttcagcac gctggctcat ggattaagca cctgattgca accgttttca ctttccgtgc
     4381 ttctcctaaa gacagaagca tcgacttcgt gtagggtttg ccagcaaatt tatttatcaa
     4441 ccgtcttaca gacgcttcca ttctggttag tggtagcgat gatgctatgc ataaaaacct
     4501 tgaagcctgc gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc taaaggcata
     4561 acccgacagg gataagacga aaaatccgag attagttaac catatatgaa tccagtaccc
     4621 gcgcaacgtg aatatttcct cgactccatc cgcgcctggc tgatgttgtt agggatccct
     4681 tttcatattt ctttaatcta ttcgagccat acatggcatg tgaatagcgc cgaaccgtca
     4741 ttgtggctga ccctttttaa tgacttcatc cactcgttcc gcatgcaggt atttttcgtt
     4801 atatccggat acttttccta catgcttttt ttacgctatc ccttgaaaaa atggtggaaa
     4861 gtacgtgtcg aacgtgtagg tatcccgatg ttaacagcca tccccctact gacattaccg
     4921 caatttatta tgctgcaata tgtcaaaggg aaagcggaaa gttggcctgg gctatcattg
     4981 tatgacaaat ataatacgtt ggcctgggaa ttaatatcac acctgtggtt tttactggtg
     5041 ttagtagtca tgacgacgct gtgtgtatgg atatttaagc gcatcagaaa taatttagaa
     5101 aattctgata aaacgaataa aaaattctcg atggtaaaac tatcggtgat ttttttatgc
     5161 ctcggcatcg gctatgcggt aataagaaga acgattttta ttgtgtatcc acccattctg
     5221 agtaatggca tgttcaattt tattgtcatg caaacgctat tttatttacc gttctttatc
     5281 ctcggcgcac tggctttcat tttccctcat cttaaagcct tgtttaccac gccgtctcgt
     5341 ggctgtaccc tcgctgcagc attggcgttt gtcgcttatt tactcaacca gcgctatggc
     5401 agtggcgatg cctggatgta cgaaaccgag tcggtgatca ccatggtcct cggcctgtgg
     5461 atggtgaatg tggtcttctc ctttggccac cgtttgctta acttccagtc agcgcgggtg
     5521 acttattttg ttaacgcatc gctgtttatc tatctggttc accacccgtt aacgctgttt
     5581 ttcggcgcat acattacacc gcacatcacc tccaactggc ttggttttct ctgtggcctg
     5641 atattcgtag tagggattgc gataattctg tatgaaattc atttacgcat cccgttactg
     5701 aagtttttgt tctctggtaa accggttgtt aagcgtgaga acgataaagc accagcccgt
     5761 taagccacat ttacaataac cattccacgg gcaatatcga cgccagtctg accataaccc
     5821 gcttccagaa actggtggcg gggtcttttt tgagaataac ctctttcttt gcatgacgat
     5881 caacccagtt gatccgtccc cacctgtcca gacggagctg ccaggccgca tcgtactggc
     5941 tctgaataaa gcgtttatca attaactgtg ccagcgtttc gctctctatc acgaagccca
     6001 tttcagtatt gagcaatgtt gaacgcggat cgaaattgaa agagccgata aacaccgttt
     6061 taccatcgat gctaaaagtt ttagcatgca ggctggcacc ggaattaccg gttatgccgc
     6121 gatcgtgtaa cgtactgctt tgttcacgcg tcggcttgag ttcatataat tccacgccat
     6181 agcggagcaa ttttttgcgc cagcgcgcgt atccggcatg gacgacagca acatcgttag
     6241 cggcaagaga attggttagg atcgcaatct ttaccccttt tctcaccatc cgtaagagtt
     6301 gcgccacacc tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg cgttcactgg
     6361 gtgagcccat gatatcgaac aggcgctgcg gtaacagtga atgccgtttt gccttgccct
     6421 cccctttcgc cggatcatca cttaataaac gtgtcttcgc ccagataagc ggcaacgttc
     6481 catcaatcag atgatttata aatggactgg attccatttt gcgtaaataa cgatgcgtca
     6541 tggcatcgtt atgccaggag gcgggtaact cgatgcgatc cgccatttca ccctccggga
     6601 catccagcac ctgctgtaag ggtgaaaccg atttgcaata ccagtagcgg gcgaaatcat
     6661 cggcaacgtc ctctaccacg gggcctattg ccatgacatc taaatccgaa aaaagtcgct
     6721 cttcccctgc accaaaatag gcatcaccaa tatttcgtcc tcccactagg gtaaccacgc
     6781 catcgacagt gaaactttta ttgtgcatcc ggcgattgag acgggaaaag tcggtgatat
     6841 atccaagcgg acgtagcaag cgaaacgaga aaggattaaa aagccggact tcaatgcgtg
     6901 gatgactgtc aagcaagcgt aaaatgtcgt caagtccggg cgtattgttg tcgtccagca
     6961 acaaacggac gcgaacgcca cgctttgctg cggctaacag ggcggaaaac agtaaccgcc
     7021 ccgacatatc gtcctaccag atgtaatact gaacatccag cgtatgctct gccatttcgg
     7081 ccagacgata ccgggcggca aacgcatcca gacttttctc cagagggaaa agaccacact
     7141 gaccgggatg ttgcgaacac agtggcagca ccgcgctcgc cagccggggc aaatcattca
     7201 tctccttgtt gggtaaggag tctttcgtag aggtgggcgt tttcttcatc ataacagaca
     7261 aagtatacct gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt tttaactgcg
     7321 atttcagccg ctgccgcacg agggtaacca taaaccccag tactgattgc aggaaaagcc
     7381 actgacgtat agctgtttgc cgccaccagt cgtaggctat tcagataggc atcctgcaaa
     7441 agctggtctt cgttttgttc accaccacgc cagactggcc cgacggtgtg tactacggct
     7501 ttagcgggaa gatcgcctgc aagcgtaata acggcatgcc ccgtagggca atcgccctgc
     7561 tgttgcctga cttttaaaca ggcatccagc agggccggac ccgctgcgcg atgaatggcc
     7621 ccatcgacgc cgccgcctcc cattaatgac ggattagccg cattcacaat cacatcaacg
     7681 gccagtttgg taatatcacc ctgcacaaca tgaatacgcg ttttcataac gcctccttac
     7741 accttcttgt ttcttaagtg tacagcaggc tttttggtta aatcgcattt ttctgctcag
     7801 caacgtgagt tgttcagaaa cttagttata ttcgatgttc attacagcca gctttttctg
     7861 tttatcaaga tactgcactt ttactgaggc tatttgcgcg tttttgacat tgccgctcat
     7921 taacgctttg ctggagtagg ttttgcccgg aatagaacca ttctatgggc agttcatttc
     7981 tacgggtgat gactgggtgc tgacgtcaca tgccggttcc acaatttggc cataaaaatg
     8041 gatcacgccc ccttgcgcgg tgcttcctgc aatggcaata aaaggaaaaa acaaactgcc
     8101 gagcataaga gagtctaaaa aaggtctgaa catttattct atttcctcaa tgattagtca
     8161 tccttgaggg ttgtgttatc catactttca ggttggctga ttcgcagcag accattctct
     8221 ccagattcat cttatgctcg atatttcaac aaattaagac ttttctgaag agggcgtcca
     8281 ttgttgtgat aaatgaagtg cctgtccatc agaaacagta acaactattt tcacccgatc
     8341 ttccggggaa atatttaaac tcaacttcgt caaagcaatg ggttgattag caggcaatga
     8401 gagggtcttt tcttgcttcg tctgactttg ccctgaactg ccttcgcgca gggacaatat
     8461 ttgtactctg cacagacaaa attgagtaag agtaacttca ggaataatgg tgtacatatc
     8521 cccttgctgg gtcgtattaa aggttatctg gctggaaagt gccgcaagga gtaataacgc
     8581 attcatattc ttctcccgaa aaaaaacagg gcttgcgccc tgtttctgta atacaaatga
     8641 tgtattaata ctgatgagcg gtcgcgttgt taccaaagcc aacctgagtc acgttgacgg
     8701 aggagttcga tgcagtctgg tcaactgtag caccgttgcc accaccgaac tgtttaacag
     8761 tcatttcaga atttttgccg ttccactgat caagagtggc gctgttaccg aagccacgtt
     8821 gggtcagatc gattgagctg tcatctgagc cctgaccaac atctgcacca ttaccaccgc
     8881 catgctgggt aatagtcaag tcagagttac gggcatcagt ttgcagagca agtgcagagt
     8941 taccgccacc gtactggtaa atgttcagct ctgaatttgg gccgctatta tta
//
LOCUS       DADMEQ010000140         8896 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.142, whole genome shotgun sequence.
ACCESSION   DADMEQ010000140 DADMEQ010000000
VERSION     DADMEQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8896
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.142"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            369..2887
                     /locus_tag="JNZ05_003475"
                     /pseudo
     CDS             369..2887
                     /locus_tag="JNZ05_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="JNZ05_003476"
     CDS             complement(2997..3845)
                     /gene="alkA"
                     /locus_tag="JNZ05_003476"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HAY8566569.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3979..5331
                     /gene="yegD"
                     /locus_tag="JNZ05_003477"
     CDS             3979..5331
                     /gene="yegD"
                     /locus_tag="JNZ05_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY8566570.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(5343..5564)
                     /locus_tag="JNZ05_003478"
     CDS             complement(5343..5564)
                     /locus_tag="JNZ05_003478"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY8566571.1"
                     /translation="MFRPNEAVTPADRQAVMAKMAAKRHRLESTLTVGATELQRFRLH
                     APARTMPLMEPLRQAAEKLAQAQADLSRC"
     gene            complement(5760..7283)
                     /locus_tag="JNZ05_003479"
     CDS             complement(5760..7283)
                     /locus_tag="JNZ05_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HAY8566572.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHGNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKARVAALDSLRQQLKKCPVSAMHVYPAHLTDCLWCTLDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKLALAALHDTARKRQK"
     gene            complement(7280..8042)
                     /locus_tag="JNZ05_003480"
                     /pseudo
     CDS             complement(7280..8042)
                     /locus_tag="JNZ05_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
     gene            complement(8039..8698)
                     /locus_tag="JNZ05_003481"
     CDS             complement(8039..8698)
                     /locus_tag="JNZ05_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY8566573.1"
                     /translation="MSEQITFATSDFASNPELRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2168 a         2357 c         2324 g         2047 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat gagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgtgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtggtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct cggtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga
     2101 accacagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
     2161 gatgattaaa gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc
     2221 ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat
     2281 tgatgaacaa aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg
     2341 acgggtattc cacgaatttt tccagcaggc cgcaaaagcg gttgccagta aaggcataag
     2401 catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct
     2461 tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta ttccggctga
     2521 agcgatttta gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt
     2581 gctcagccag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga
     2641 ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat
     2701 gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc
     2761 agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg
     2821 tgatgtgatt gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat
     2881 taactgagag agcgtttgcc cattgcctga tgcgatgcta acgcgtctta tcaggcctac
     2941 aaatcgctca ttccgcaggc gggataaggc gctcgcaccg cttccggcga ccaacgtcat
     3001 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag
     3061 ggcttccagc gctcggcata acggcggatt tgtgccggtg tcatccccag aaatcgctgt
     3121 ttaatcagat aatcatccgg cagaaaaaca tctttcgcct gccagccgcg caaggcgaaa
     3181 taattcgccg tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc
     3241 acatcgcccg gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc
     3301 agcgcctctg cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctgct
     3361 gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca
     3421 taaagctgtg ccactctggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct
     3481 aaaatcgccc gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc
     3541 cgcgccgcgc ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
     3601 agcaggcggc tcattttcgc cagacactca gcggcaacgg gttctaaacc tgcacttaaa
     3661 tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg
     3721 cccactgcca gactacgggc ataataactg tccgcgaccg tttccacacc actcaccgca
     3781 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcaggatg
     3841 tacatcgcat ctccttattc atccgctttc agcataaacg ttattcagac gttacgcttt
     3901 gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac
     3961 cggcatgggg attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg
     4021 gcggtcatgc gtgacggtaa accgcatttg ctaaaaatgg aaaacgacag cacgctgctg
     4081 ccttcaatgc tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat
     4141 gacgttccgg cagacgacga tgaaacgcag gcgttgttac gacgggcgat tcgttataac
     4201 cgcgaagaag atatcgatgt tacagcgaaa agcgtgcagt tcggtctttc ctcactggca
     4261 cagtacattg atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
     4321 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
     4381 ttgcacattc gccagcaggc gcaggcgcag ctgccagaag caattactca ggcggtgatt
     4441 ggtcgcccga tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcagggg
     4501 attctggaac gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gtacgagccg
     4561 gtagcggctg ggctggatta cgaagccacc ttgcaggaag aaaaacgggt gctggtggtg
     4621 gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg
     4681 cgtctcgatc gtgaagccag tctgctgggt cacagtggtt gtcgtattgg cggtaacgat
     4741 ctggatatcg ccctagcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc
     4801 gaaaaaggca tcgccctgcc gatcctgccg tggtggaatg cggttgccat caacgacgtt
     4861 cctgcacaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
     4921 gatgcccgcg aaccggaaaa agtggccctg ttacagaaag tctggcgtca gcgtttaagc
     4981 tatcgcctgg tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt agcggaaacc
     5041 cgcgcctcac tgccgtttat cagcaatgaa ctggctacgc tgattagcca gcgagggctg
     5101 gaaagcgccc tcagccagcc actggcgcgg attctggaac aggtgcaact ggccctggat
     5161 aacgcccagg aaaaaccgga cgttatctac ctgaccggcg gtagcgcccg ttctccgctg
     5221 attaaaaaag cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt
     5281 ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tatttcgtta aaagaaaatg
     5341 gctcagcagc ggcttaaatc agcctgcgcc tgagccagtt tttctgccgc ctgacgtaac
     5401 ggttccatca acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg caactctgtc
     5461 gcgccgacag tcaacgtcga ttccagccga tgtcgtttgg cggccatttt cgccattacc
     5521 gcctgtctgt ctgctggcgt taccgcttcg ttcggcctga acacaaaacg gcgttcacag
     5581 ctcgctttcc agtcgatgac cgcctgggtc agatgatcac caaacccttt aacttgctta
     5641 acgccacgac gggtaacacc tgctgctgtt tcaataccaa aagaccataa cgccgcttta
     5701 cgcgcagggc caacgccggg aatagaggca acatcaataa aaaatccctc cagaaacttc
     5761 tacttctgcc gtttccttgc agtgtcatga agtgctgcca gagcgagttt ctcttcttcc
     5821 ggtaacccga gaatttcgtc cttcattttt tcgagcatcg tccgtttggc gataaaacct
     5881 tccaggccgc ctacctgctg gatctggcgg actaaatggt catagtccat tttcgcacga
     5941 ttaaatgcct cacggcgttg ctgaacttct gctttgtacg ctttgcttgt cagactgcca
     6001 ataatccaga tagccgccag cacaggaacc aaaataatat aacgcggttc tgcctgaagg
     6061 ccgcaaagca gcagcgataa tgctgacagt gcgatctcaa gcagaatgat gtattcgcgc
     6121 cgtaacaggc ctaaaggaag cggcctgcca gtcggttgga aatgatcggg taatggcagt
     6181 tgcaatgctg gcggtgctac tgacgccatc accatcgccc agacttttgc cagcacaaaa
     6241 tccccgccgg tggtaatgac ctcttcgccg agatcaataa aatagataac gccttgatta
     6301 tccagcgtac accacaggca gtcggtcaaa tgagcgggat aaacatgcat tgccgaaacg
     6361 ggacatttct ttaactgttg gcgcagagaa tcaagcgccg ctaccctcgc tttagccgtc
     6421 ggacgcccgg ttgccacgcc actttccgtg aatgcctgct gaaacatggc ttcaacatca
     6481 ctcggtaaca tcgatagcgg aatcgatcgt ggcggcggtt ttaaaccacg tcgctgatta
     6541 tctgacgcgt aggcataacg gaaatgggta atatccgtct ccagcgcatt acccgcatca
     6601 gagataagcg gcacaccaga ataaggatgc cggccaccaa acaagacgtg aaaaatcagc
     6661 aacgcaaggc caaaattacc gtgattttct gtgcgttcaa agccgacaaa tgacgacaag
     6721 gtttgtagct ctggcggcgt aaaatgcgac acgccgactt cgcataaatg cagtgtgcca
     6781 ttggcgttaa tctgaaagga gtcgctatca atcaacacca ctttgctgtc gcgacctacc
     6841 ataaaactgt tctggtttac gtcacccacg acatgcccgt gctcgtgaac cgtagcaaaa
     6901 gatgaagcaa tattgcgcgc aacatagagt agaaaatccc acgcacaatg agggtaacgt
     6961 tgacgacgat gtgccgggct atagatcata tgaatcggtt ctttaccaga aacttttggc
     7021 atcataaaac cgataacttt tccgcctcgt ccaccgtgaa gcgttgcctg cggccaggcg
     7081 acataattca acaattgcgc gtcagctgtc gcagccataa aggcaagttt gtcctgtttt
     7141 aaggcgggtg gcggcgtgtg ataaatcttg gcgacgctat cgacaaactc attgatatca
     7201 taaaccgcgc cttcaccgcc tttgcccagt tcacggcctg gcgtaacgca ctcgccagta
     7261 gcagtatata aagtgggttt catggtaacc acagcgccag tgccaacgtt ttatcatcat
     7321 ccgtacgctc gtttaccgcc gggctggaca aaaactgctt taacaattca ggtaataaat
     7381 cgagttgttc ctgcgttgct gacgccagtc cattgaaaaa cggggtaaag aaaggcacat
     7441 gcggagaatt atccaacata tttaacgcca gtcgctggat accatccgta aatgctgcaa
     7501 ctttatgcac acgctcagtg ctggtgaaag tttccagtct ggaaacggca tcttcatcgg
     7561 taatgaagtg cgtcatattg gcatattcac ccaccatcgg cgtaagcggc aattgcagac
     7621 cgtgaccaaa atccaccacc acgccgccat cgccaatctg cataatcaag gtgccattag
     7681 ccgatgaaat caatcccaga aacgtacagg caaaatcgcg aactgctaat tctttggctt
     7741 ccgcctcggc aataaagtcg ctgacgaacg gtcagcacaa tatccgtggc gaggatatca
     7801 ttaagcccca attccccacc ctgcactttt tgcgacatat aagccatcgc ttcattgacg
     7861 gcgagcatcg cgccttcgcc cccctgcgaa acgctacctg cgccatcagc aaggaacatc
     7921 accagtaatg gctgctggtc atttagccag gcaacctgca tctggcaggc atcctggcaa
     7981 ggcaagtccg cgctgatgtg cgacgttcca acagcggatg cataaaccag acgccagctc
     8041 acactgacgt ccagcctttt ggcgcttcca gcacaacttc cgttcccggc gtggagcggg
     8101 aaaccgaacg cagcgaactg gataaccagc taaacagttc acggaattgt aatccttgca
     8161 gaggcaaagg ctgacgaacg ctgatttgtg ccagagtctt catatccgcc ccctgaacac
     8221 cgatggaaaa gaaagcaaac cttttatctt cttccccccg aaacactttg ttggctgcag
     8281 cctgccactc atcggttggt gcaccatcgg taatcaggaa aatccacggg cgataatacg
     8341 aaataccatt tgcacgatac tcacgtttcc gctcctctac catatccagg gctttggtaa
     8401 tggccgcacc cattggtgta tcgccctggg caaacaggat gggcggaaaa aaattagcgg
     8461 cgctggtaaa gggttgttcc acatgcaccg ggccgaacgt cacaatgccg agttccactc
     8521 tttttaaagc cagcgaatcg gcaagcagct catcgcgaaa ggtaaccaat ccggtgttaa
     8581 gttcattaat aggtctgcca ctcatcgagc cagaaacatc cagcagcaga atgcaggggc
     8641 aacgtagttc cgggttactg gcaaaatcgc tggtggcaaa cgtgatttgt tcgctcataa
     8701 gaaatccttt cttagagaat tattgaggtc cggtatcata ccgactgcaa taatgaaata
     8761 agagcaaacg aagaaacttt cagaattagt cccggcggcg cagaaaattt tgctattcgc
     8821 ctcgcaagaa cggagagccg tcgattgaca ctaagggcgg agtgacataa tttcaggagt
     8881 gagggttagg gagagg
//
LOCUS       DADMEQ010000141         8673 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.143, whole genome shotgun sequence.
ACCESSION   DADMEQ010000141 DADMEQ010000000
VERSION     DADMEQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.143"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..323)
                     /locus_tag="JNZ05_003482"
     CDS             complement(<1..323)
                     /locus_tag="JNZ05_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY8566574.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVALLWVNGE
                     NFRTL"
     gene            complement(333..881)
                     /locus_tag="JNZ05_003483"
     CDS             complement(333..881)
                     /locus_tag="JNZ05_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAY8566575.1"
                     /translation="MGLPPLSKTPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYTEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(881..1588)
                     /locus_tag="JNZ05_003484"
     CDS             complement(881..1588)
                     /locus_tag="JNZ05_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY8566576.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1603..2280)
                     /gene="ydjY"
                     /locus_tag="JNZ05_003485"
     CDS             complement(1603..2280)
                     /gene="ydjY"
                     /locus_tag="JNZ05_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HAY8566577.1"
                     /translation="MSQHYSVSWKEGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2285..2995)
                     /locus_tag="JNZ05_003486"
     CDS             complement(2285..2995)
                     /locus_tag="JNZ05_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY8566578.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYNLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(3162..3968)
                     /gene="xthA"
                     /locus_tag="JNZ05_003487"
     CDS             complement(3162..3968)
                     /gene="xthA"
                     /locus_tag="JNZ05_003487"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAY8566579.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGY
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            4414..5634
                     /gene="astC"
                     /locus_tag="JNZ05_003488"
                     /pseudo
     CDS             4414..5634
                     /gene="astC"
                     /locus_tag="JNZ05_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            5631..6464
                     /gene="astA"
                     /locus_tag="JNZ05_003489"
                     /pseudo
     CDS             5631..6464
                     /gene="astA"
                     /locus_tag="JNZ05_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
     gene            6460..7389
                     /locus_tag="JNZ05_003490"
                     /pseudo
     CDS             6460..7389
                     /locus_tag="JNZ05_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            7386..>8673
                     /gene="astB"
                     /locus_tag="JNZ05_003491"
     CDS             7386..>8673
                     /gene="astB"
                     /locus_tag="JNZ05_003491"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HAY8566580.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFKDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMLLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQSVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQ"
BASE COUNT         2022 a         2227 c         2384 g         2040 t
ORIGIN      
        1 tttaaggtgc ggaagttttc gccgttcacc cagagcagag ccaccgagcc gcccgtttta
       61 cgaccggcag cagcttcggt ctgaatgcgc ttcaccgcgt ctgcggcatc cgcgagacga
      121 acaatcttca ggtttatagc gtaatgggtt ttcatctcgc cgctaaccca gtcgagatag
      181 cggttaattg cggtatcgcc gccccaggcg ttaaaccaga cggtttgccc tttggcctca
      241 tttttaattt cctgccagtc actggcatgt gttgccaaag aaaacagcga taacaatccc
      301 agcaaccacc cacaatggcg catcttccct ccttaagaac gtttttccgg aatacggcac
      361 agcccctgag cgggaatgtc catggcagaa ttaaaacgag ccgacaggtt ttgcaggccg
      421 atcaatgccg tcagttcggt gagcgcctga gcgtcaaaat gcgcagccag tcgggtacgc
      481 agggcatcat cgactgttgg cggtgtgacg cttgcggctt cggtgtactc cagcgccagc
      541 cgttcttcat cgctaaagag cgggctttgc cgccagtcag ccactgccag cagtttatcg
      601 gtgctgccgg tacgctcggc gactttcata ctggtgatat ccacgcaaaa ctcacacagg
      661 cacatttgcg caatgcgcgc gctgacaagc gatcgcacta ccggatcgag cggtgagcgt
      721 ttgcgctcca gccacccaac aaacattgac accaaataaa agataaacgg gatccgcccc
      781 caccagcgaa tcgggcttag cacctcgccg taatggcgac gatgcagcca cgcctgcgga
      841 cgtaaaatga aaggagtttt gctaagcggc ggtaaaccca tcagtcattc ctcttctggc
      901 gctcaagcca tattttcttc gccataaaaa tcaccacggt cagggcaaac agaataaaca
      961 gttcggtgac aaaccagaaa gtaccgcctg ttaacatgct gcccgcccag gaataaacaa
     1021 tagtcgccgg taattgacca agcccggtgg cgataaaaaa cgagcgaaaa cgtattgaag
     1081 tcagaccggc agcatagctg attggatcga aagggacaaa aggcaataac cgacagacca
     1141 ggatggtgtg tttgccgtag cgagtgaaaa agccgtccat actgtcaagc acggttttgc
     1201 cggttaattt ttccaccact tcgcggccca tcactctggc gataaaaaag cacagcgccg
     1261 cgccggccat cgaactggtc cacgacagca agccgcccca gaacgcgcca aacagcgacg
     1321 cattggcaaa ggtgatcaaa aacgcaggta gcggtgcagc aatggcctgt aaaatcatca
     1381 acaagaacga gacaactgcc gccagtgcgc cgtaagactg aataaagcgt tctataccct
     1441 gttggtccac ggcggcaaac gccgcaacgc tgcgattgat aaactcatgg acgccgggaa
     1501 gcagcgccca tgcgagcagc atagcgaaca acaggatgat gagggttata cggtagtacc
     1561 agattttacg cgattgtatc ttcatcatcc tttatcctgg ctttattcgg tgattttaaa
     1621 ggtaaccgtt gccagcgtgt tatccgccgg gagaactgag gcattgcctt tgaatttcac
     1681 ttcgccgcgt ttttccaccg caccataagt gtatgttgca ttgctgacga tgccgaccgg
     1741 gcagctatcc agacacacca ggcaacccgt tttcttctct tctgctgccg ttaaattacc
     1801 gccaaagcgc atgtccagtt tcttgccatt actgtcaaca atcacttcat cgaaggaata
     1861 cgcttttgcc gccccttgcc agttgaccga aatatccagt ttgctgcctg tgacatgagt
     1921 cgtttcttta ttatccatcg tcatgttttc gcccggcgta ccacctgcct ctttcagggc
     1981 ttcataaaat gctttcgggg tcgcataagc cataaacagc gatttatgtc cgttggagcc
     2041 atctttaaac acaataccgt gacgagtgtc gtcggtgagg aaacgaccat taatttgcac
     2101 cagcatggtt accgtatggt tattggcatc gacgcgcagc ggttggctgt tcgaaagtcc
     2161 gtcgtattcc acttttgccg ccgcattctc tttttgatcg cagccgctaa gcccggcaac
     2221 agccagtaag cagagtgcag ccagtccttc tttccatgac actgaataat gttgcgacat
     2281 cgttctatcc ttcatttttt gggtgagtaa gtgggctgcg gcgcgaagca gacagatcca
     2341 catgtttgtg tcgggcgtag agttttgcga gctggatgag aataaacagc gccaggcccg
     2401 ccagacagag ttgtaaaata aagcgcagcg taatgccttc attggcgaga tcgcttgcca
     2461 tcacggtata aataacaata ccgggcaggg tcgtgagtgc cgaaataagg gtataaggcc
     2521 agaaggcgat ggtggttaat ccgtaagcgt aattttgaat attgtaagga aacaacggga
     2581 ttaagcgggt cagaataaga aaatcaatac cgttacgcgc aatgcctttt tcaatggcct
     2641 ggaaggtatg gctatggccc acgtatttca gcagtaaatc acgtcccagc cagcgcgcca
     2701 gcaggaatga gcacgaggag gccagcgttg cggcaattaa tgagagtagt gtccctaaga
     2761 gcgggccaaa aactattcct ccagcgatca ccagtatgct tcctggtaac agaaagaggg
     2821 gggcattgat gaataacaga atatagaggt tataaccgaa caatccgctc tggcgaatga
     2881 gtgtctgtaa gtggggtaaa tcggtgagca gatcgaataa accgaaagcg tggatagcgt
     2941 aaatgaccag cgcaaaaata agacaggcaa acagaaagtt acgctcagcg ttcatcgtgg
     3001 agtttcctat atcaaacttg ataatagaga agtggtcaga caactaaaaa cgcagtgacg
     3061 gtataacaaa ggacggcagg caacaaatca ttactgacag tttgttgatt tttctcaagg
     3121 ttaattctcc tgacccagtt tgagccagga gagctgctaa attagcggcg gaaggtcgcc
     3181 cagacggggg cgtgatcgga cggtttttcc atgctgcgaa tttcatagtc gatgccggtt
     3241 tctacgcaac attctgccag cggttggctg gcgagcagca ggtcgatgcg cagaccacgg
     3301 ttatcgtcaa aaccttttga gcggtaatca aaccatgaga aacgatctgc tgtttgcgga
     3361 ttagcatggc ggaaggtatc gaccaccccc cagctcatca gcctgtccat ccattcgcgc
     3421 tcttccggca ggaaagagca tttaccggta cgcagccagc gcttacggtt ttcttcgcca
     3481 atgccgatat ccagatctgt agggctgata ttcatatcgc ccataatcag taccggatta
     3541 tcacgtttga gttcggtttc caggtagttt tgcagattct gataaaactg cgcttttgcc
     3601 gggaatttta tcggatggtc gcggctttca ccctgcggga agtaaccgtt gatcacggtg
     3661 acataaccca gcagtgaggg gatttccgcc ataataatcc gccgctgcgc ctcttcgtcg
     3721 tcaccgggaa agccgcgacg cacggcaatc ggcgtctctt tggtcagcag cgccacgcca
     3781 taatggcctt tctgcccgtg ataaaacacg ttgtagccga gcttcgccac ctcttcgagc
     3841 ggaaacatat cgtcatgaac ttttgtctcc tgcaggccaa tcacatccgg ttggtgcttt
     3901 tcgacgatgg cttcaagctg gtgaggtctg gcgcgcaggc cgttgatatt aaaagagaca
     3961 aatttcatag tcgctgccat ttattcagat aaagagtgcg tggatggtag cagaatttcg
     4021 cgttgcttac cgcctgttgt taagtgattt gatcgatggt tgttaatgat gtcaacgatg
     4081 gcgcaaaaaa tgcccgcttt tggtgcgcgc tgcgtcagaa tggcgcagta atttccagta
     4141 aatttcgtca atgatcacaa ttccagaatt atattttact tctgcataac attgcgtttt
     4201 ttattctttt tattcaccca catcgcaaac gtattcactt tatatgcact ttaaatgcat
     4261 atggtttggt tataactcct tgatttccag ttagcctccg ccgtttatgc acttttatca
     4321 ctggctgaca cgaaccctgc aatctacatt tacagcgcaa acattactta ttgttaacat
     4381 acaaataacg aattatttac tgtagaggtc gctatgtctc agccaattac gcgtgaaaac
     4441 tttgatgaat ggatgatacc tgtttacgct ccggcaccct ttataccggt acgtggcgaa
     4501 ggttcgcgct tgtgggatca gcaggggaaa gagtatatcg acttcgcggg tagcattgcg
     4561 gtgaacgcgc tgggccatgc gcatccggaa ctgcgtgaag cgctgaacga acaggcgagt
     4621 aagttctggc ataccggcaa cggttacacc aacgagccgg tactgcgact ggcgaaaaaa
     4681 ttgatcgacg ccacgtttgc cgatcgcgtc ttcttttgta actccggtgc ggaagccaac
     4741 gaagcggcgc taaaactggc gcgtaaattc gctcacgacc gctacggcag ccataagagt
     4801 ggcatcgtgg cgttcaaaaa tgcgtttcat ggtcgcacgc tgtttactgt cagtgcgggt
     4861 gggcagccag cctattcaca ggattttgcg ccactgccgg cggatattcg tcatgctgca
     4921 tataacgata ttaactctgc cagcgcgctg attgacgacg ccacctgtgc ggtgattgtc
     4981 gagcccatct agggcgaagg cggtgtggtg ccagccagca acgcgttttt acaaggtctg
     5041 cgtgaattgt gtgaccgcca caatgccttg ctgatttttg atgaggtaca aaccggcgtc
     5101 gggcgcactg gtgagctgta tgcctatatg cactacggcg tgacgcccga tctgttaacc
     5161 accgccaaag cgctgggcgg cggtttcccg gtcggtgcgt tgttggcaac cgaagagtgc
     5221 gcccgcgtga tgaccgttgg cactcatggc accacctatg gcggtaatcc gctggcctcg
     5281 gcggtagcag gcaaagtgct ggagctcatc aacacaccag agatgcttaa tggcgttaaa
     5341 cagcgtcacg actggtttgt tgagcgtctt aatactatta atcaccgcta tggtttgttc
     5401 agtgaagttc gcggcttagg tttgttgatt ggctgtgtac tgaatgccga ttacgccggg
     5461 caagcgaaac agatctctct ggaagcggcg aaagcaggcg tgatggtact gattgcgggt
     5521 ggcaacgtgg tgcgttttgc gcctgcgctc aatgtcagcg aagaagaggt gacgaccgga
     5581 ctggatcgct ttgcagctgc ttgcgaacac tttgttagcc gaggttcatc atgatggtca
     5641 tccgtcccgt tgagcgctca gatgtctcgg cgctgatgca gcttgctagc aaaacgggcg
     5701 gcggcctgac gtcgcttccc gccaatgaag ccacgctttc ggcgcgtatc gaaagggcaa
     5761 tcaaaacctg gcaaggcgaa ctgcctaaaa gcgagcaggg ctatgtgttc gtgctggaag
     5821 atagcgagac aggcaccgtg gcggggattt gcgctattga ggtggcggtt gggctgaacg
     5881 atccctggta caactatcgc gtcggcacgt tggttcacgc ctcaaaagag ctgaatgtct
     5941 ataacgcatt gccgacgctg tttcttagta acgatcacac cggcagcagc gagctgtgca
     6001 cgctgtttct cgacccggac tggcgcaaag agggcaacgg ctatttgctg tcgaaatcgc
     6061 gctttatgtt tatggcggct tttcgcgaca agtttaatga caaagtggtt gctgaaatgc
     6121 gcggggtgat tgacgaacac ggctattcac cgttctggca aagcctcggt aaacgcttct
     6181 tttcgatgga ttttagccgc gccgattttc tctgcggcac cgggcaaaag gcatttattg
     6241 cagaactgat gccgaaacat ccgatctata cccacttttt atcccaggaa gcccaggacg
     6301 tcatcggtca ggtacatccg caaaccgcgc ctgcccgcgc ggtgctggag aaagaaggtt
     6361 ttcgttaccg caactatatc gacatcttcg acggcgggcc aacgcttgag tgtgacatcg
     6421 accgcgtgcg cgccatccgt aaaagtcggc tggtggaagt ggcagcaggc tggcttgccg
     6481 ccgggcgtgc tgaacctggt gcagggcggg cgtgaaacgg gtcaggcgct gagtgcgctg
     6541 gaggatctcg acggtttgct gtttaccggt agcgccaata ccggctacca gctgcatcgc
     6601 cagctctccg gtcagccgga gaaaattctc gcccttgaga tgggcggtaa taatccgcta
     6661 attatcgatg aggtggcgga tatcgacgcg gctgtccatc tgaccattca gtcggcgttt
     6721 gtcacagccg ggcaacgctg cacctgcgcc cgccgtttat tgctcaaaag cggagcgcag
     6781 ggcgatgcgt ttcttgctcg tctggttgcc gtcagccagc gattaacgcc gggcaactgg
     6841 gatgacgaac cgcagccgtt tattggcggg ctgatttctg aacaggccgc acagcaggtg
     6901 gttactgcct ggcagcaact ggaagcgatg ggcggacgaa ccctgcttgc gccgcgctta
     6961 ttacaagcag ggacatcgtt gctgacacca gggatcattg aaatgacggg cgttggcggt
     7021 gtgccggatg aagaggtgtt cgggccgctt ttgcgcgtct ggcgttatga tacttttgat
     7081 gaggcgattc ggatggcgaa caacactcgc ttcggactct tttgcggtct ggtttccccc
     7141 gagcgggaaa aattcgatca actgttgctg gaggcgcggg cggggattgt taactggaac
     7201 aaaccgctta ctggtgctgc cagtaccgcg ccattcggcg gcattggtgc ttccggtaac
     7261 catcgcccca gcgcctggta tgccgcagat tactgcgcat ggcctatggc gagcctggag
     7321 tcggactcgt taacattgcc cgccacgctt aaccccgggc tggatttttc cgatgaggtg
     7381 gtgcgatgaa cgcctgggaa gttaatttcg acgggctggt agggctgacg catcattacg
     7441 cgggcctgtc gtttggtaat gaagcctcta cccgtcaccg ttttcaggtg tctaacccgc
     7501 gactggcggc aaaacagggc ttactgaaaa tgaaagccct tgccgatgcg ggattccccc
     7561 aggccgtgat cccgccgcac gagcgtccgt ttattccggt gctgcgtcag ttgggattca
     7621 gtggtagcga tgagcaggta ctggaaaaag ttgcacgcca ggcaccgcac tggctttcca
     7681 gcgtcagttc cgcttcgcca atgtgggtag ccaatgcggc aacgatcgcg ccatctgccg
     7741 atacgctgga tggcaaagtg catctcaccg ttgccaacct gaacaataaa tttcaccgtt
     7801 cgctggaagc gcccgtcact gaatcgctgt taaaagcgat ttttaaggac gaagagaaat
     7861 ttagcgtcca ttcggcgttg ccgcaggtag cgttgctcgg tgatgagggg gcggcaaacc
     7921 acaatcgtct cggcggtcat tacggtgaac cgggtatgct actttttgtc tacgggcgag
     7981 aagaaggcaa tgatacccgg ccttcccgtt atccggcgcg acagactcgc gaagccagcg
     8041 aggcggtggc aaggcttaat caggtgaatc cccaacaggt gattttcgcc cagcaaaacc
     8101 cggacgttat cgaccagagc gtttttcata atgacgtgat tgccgtgagt aaccgccagg
     8161 tgctgttttg ccaccaacag gcgttcgctc gccagtcaca gttactggca aacctgcggg
     8221 cgcgggtgaa cggttttatg gcgatagaag ttccggcaac tcaggtttcc gtgtcggatg
     8281 cggtgtctac ctatctgttt aacagccaac tgttgagccg cgatgatggt tccatgatgt
     8341 tggtgctgcc tcaggagtgt cgggaacacg ccggagtatg gggttatctc aatgaactcc
     8401 ttgccgctga caacccgatt agcgaactaa aagtctttga cttacgtgaa agcatggcga
     8461 atggcggcgg cccggcgtgc ctgcggttgc gggtggtatt gacagaagaa gaacgccggg
     8521 cggtgaatcc ggcggtgatg atgaacgata ccttgtttaa tgcgctcaat gactgggtgg
     8581 atcgttacta ccgcgatcgt cttactgctg ccgatctggc cgacccgcaa ttgctgcgcg
     8641 aagggcggga agcactggat gtattgagcc aat
//
LOCUS       DADMEQ010000142         8351 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.144, whole genome shotgun sequence.
ACCESSION   DADMEQ010000142 DADMEQ010000000
VERSION     DADMEQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.144"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..158
                     /locus_tag="JNZ05_003492"
     CDS             <1..158
                     /locus_tag="JNZ05_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="traD domain protein"
                     /protein_id="HAY8566581.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(167..565)
                     /gene="vapC"
                     /locus_tag="JNZ05_003493"
     CDS             complement(167..565)
                     /gene="vapC"
                     /locus_tag="JNZ05_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HAY8566582.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(565..792)
                     /gene="vapB"
                     /locus_tag="JNZ05_003494"
     CDS             complement(565..792)
                     /gene="vapB"
                     /locus_tag="JNZ05_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HAY8566583.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            874..1293
                     /locus_tag="JNZ05_003495"
     CDS             874..1293
                     /locus_tag="JNZ05_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005030230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HAY8566584.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRHHPCGGTGLHTGDDGRTVRNGADR"
     gene            1280..6145
                     /gene="traI"
                     /locus_tag="JNZ05_003496"
     CDS             1280..6145
                     /gene="traI"
                     /locus_tag="JNZ05_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HAY8566585.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQYNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIA
                     ADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGA
                     ARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARL
                     AGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDS
                     RSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQGETQVVRISSLD
                     SSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAT
                     LPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLY
                     SSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALP
                     VLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLL
                     VSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMILETSDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFL
                     HDTQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIA
                     PGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQVPR
                     LEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEA
                     REQTLIVTHLNEDRRVLNSMIHDAREKAGELGKVQVMVPVLNTANIRDGELRRLSTWE
                     NNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTIRV
                     GTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLA
                     YAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTD
                     AINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPA
                     RFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQF
                     VALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIP
                     DNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTEQA
                     VREIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDMVR
                     DLQKEKTPGGD"
     gene            6165..6911
                     /gene="traX"
                     /locus_tag="JNZ05_003497"
     CDS             6165..6911
                     /gene="traX"
                     /locus_tag="JNZ05_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HAY8566586.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6966..7526
                     /locus_tag="JNZ05_003498"
     CDS             6966..7526
                     /locus_tag="JNZ05_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HAY8566587.1"
                     /translation="MIEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7657..7869
                     /locus_tag="JNZ05_003499"
     CDS             7657..7869
                     /locus_tag="JNZ05_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HAY8566588.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8053..>8351
                     /locus_tag="JNZ05_003500"
     CDS             8053..>8351
                     /locus_tag="JNZ05_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAY8566589.1"
                     /translation="MLHYSGGLKYRWHLSDMENNMRKYIPLVLFIFSWPVLSADIHGR
                     VVRVLDGDTIEVMDSLKAVRIRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1917 a         2096 c         2673 g         1665 t
ORIGIN      
        1 tcagtgaatc cggtgaagtg gtggatatgg ccgcttatga ggcatggcag caggaaaatc
       61 atccggacac ctggcagcag atgcagcgtc gtgaagaggt gaacatcaat gtgcaccggg
      121 aacgcgggga ggatgttgag ccgggagatg atttctgatg aacaggtcag ctccagtctt
      181 cagttctcag gccgcccaca cgttcaaatt cccgggtgtt attagtcaca ataatcagcc
      241 cccgactgcg ggcatgacct gcgatcattt gatcaaatgg cccgacaggg cgtccctgac
      301 gggcaagttc tgctcttatc tggccggtgt gtgtggctgc agcagcgtcg taatccagaa
      361 catcaatgcg ggaaacaaat ccctcgatca cagcgagatt acgttcaggc atctggcttt
      421 tttctgcacc atatatcagc tccatcaggg tgaccgaact gatacacatt ttcccctggt
      481 tcaggttaaa acgctccctg acgctggcgg gtttgttctt tatcgtaaaa atgcagatgt
      541 tggtatcgag cataaacttc agcatcagaa tgactccctt tcctgcatgc cgggctgttc
      601 cctgttatcc ataaaatcgg tgctgacgct gtgtccgtcg aaccattcgt cccatgtctc
      661 tccggctggc gtaatgattc tggttcgtcc gacagcaatc acttcaacgc gttttacgtt
      721 ttccggcaat gcaaccgctt ttggcagtct gaccgcctgg ctgcggttgc tgagaaatac
      781 ggtggtttcc attatttgcc tccttatgtg gatatgtctt gtgtatatct attgtgggat
      841 tgataccggg atatgtcaaa gggatatacg tttatgatga gtatcgcgca ggtcagatcg
      901 gccggaagtg ccggtaatta ttataccgac aaggataatt actatgtgct gggcagcatg
      961 ggagaacgct gggccggcca gggggctgaa caactggggc tgcagggcag tgtcgataag
     1021 gatgtcttta cccgccttct ggagggcagg ctgccggacg gggcggattt aagccgcatg
     1081 caggatggca gtaacaggca tcgtcccggc tacgacctga ccttctccgc tcccaaaagt
     1141 atctccatga tggccatgct cgggggggac aaacgtctta ttgaagcaca taaccaggcc
     1201 gtggattttg ctgtccgtca tcacccgtgt ggaggcactg gcctccacac gggtgatgac
     1261 ggacggacag tcagaaacgg tgctgaccgg taatctggtg atggcactgt ttaaccacga
     1321 caccagtcgc gatcaggaac cacagttaca cacgcatgcg gtggtgacta atgttacgca
     1381 gtataacggc gaatggaaga cactgagcag tgacaaagtg gggaaaacgg ggttcattga
     1441 gaatgtgtac gctaatcaga ttgcctttgg caggctctac cgggaaaaac tgaaagagca
     1501 ggttgaggcg ctgggctatg aaactgaagt ggtcggtaag cacggtatgt gggaaatgcc
     1561 gggtgtaccg gtggaggcct tttccggacg ctcacagact atccgggagg ccgtcgggga
     1621 agacgcctcg ctgaaatccc gggatgtggc ggcgctggat acgcgtaaat ccaaacagca
     1681 cgtcgatcct gaagtcagaa tggccgagtg gatgcagacg ctgaaggaaa ccgggttcga
     1741 catccgggca tatcgtgacg cagcggaaca acgggcatat acccgcacgc agacacccgg
     1801 acccgcttca caggacgggc cggatgtgca gcaggcggtg acacaggcca ttgccggatt
     1861 aagtgaacgc aaagtgcagt ttatgtatac ggacctgctg gccaggacgg tcggcatact
     1921 gccgccggaa aatggtgtga ttgaacgggc acgcgccggt atcgatgagg ccatcagccg
     1981 tgagcagctt atccccctcg atcgtgagaa ggggctgttc acgtccggga ttcatatgct
     2041 cgatgagctg tcagtccggg cactcagtcg tgacatcatg aaacagaacc gggtgaccgt
     2101 acatccggag aaaagtgtcc cccggacggc cggttacagc gatgccgtca gtgttctggc
     2161 acaggaccgt ccgtcgctgg ccattgtgtc cgggcagggc ggtgcagccg ggcagcgtga
     2221 gcgggtggct gaactggtca tgatggcccg ggagcagggg cgggaggtgc agattatcgc
     2281 tgctgaccgt cgctcgcaga tgaacctgaa gcaggatgaa cggctgtccg gtgaactgat
     2341 aaccggacgt cgtcagttgc tggaaggcat ggccttcacg ccgggcagta ccgttatcgt
     2401 cgaccagggc gaaaaactct ccctgaaaga gacgttaacc ctgctggatg gtgccgcacg
     2461 tcataacgta caggtcctga taaccgacag cgggcagcga accggtacag gcagtgcact
     2521 gatggccatg aaggatgccg gggtgaacac atatcgctgg caggggggag aacagcgacc
     2581 ggccaccatc atcagtgaac cggaccgtaa tgtccgctat gcccggctgg ccggagattt
     2641 tgcggccagc gtgaaagccg gagaagagag cgtggcacag gtcagcggag tacgggaaca
     2701 ggccatactg acacaggcca ttcgcagtga gctgaaaaca cagggcgtgc tcggacaccc
     2761 ggaggtaacc atgaccgccc tgtcaccggt ctggctggac agccggagcc gttatctgcg
     2821 ggatatgtac cgcccgggaa tggtgatgga gcagtggaac ccggagacac gcagtcatga
     2881 ccgctatgtg acagaacgtg tgacggcgca gagtcacagc ctgaccctgc ggaatgcgca
     2941 gggcgaaacg caagtggtgc gtatttcttc cctggacagc agctggtcgc tgttccggcc
     3001 ggaaaaaatg ccggtggcag acggtgagcg tctgagggtg acagggaaaa ttcccggact
     3061 ccgcgtctcc ggcggtgacc gcctgcaggt ggcatccgtc agtgaagatg cgatgacggt
     3121 tgttgtgccg ggacgggctg aaccggccac cctgcctgtg agcgattcac cgttcacggc
     3181 actgaagctg gagaacggct gggtggaaac gcccgggcat tccgtcagcg acagtgcgac
     3241 ggtttttgcc tccgtcacac agatggcaat ggacaatgcc accctgaatg gtctggcccg
     3301 cagtggtcgt gatgtccggc tgtattcctc actggatgaa acccgtactg cggaaaaact
     3361 tgcccgccat ccctccttta cggtggtttc tgagcagata aaggcgcgtg ccggtgagac
     3421 attgctggaa accgctatca gtctgcagaa agccgggctt cacacgccgg cacagcaggc
     3481 cattcatctg gcccttcctg tgctggaaag taaaaacctg gccttcagca tggtggacct
     3541 gctgacagag gcgaagtcgt ttgctgcaga aggaaccggt tttgctgacc tgggagggga
     3601 aatcaatgcg cagataaaac ggggtgattt actgtatgtg gatgtggcaa aaggctatgg
     3661 cacaggcctg ctggtttccc gtgcgtcgta tgaggcagaa aagagcattc ttcgccatat
     3721 tctcgaaggt aaggaggcgg tcacgccgct gatggagaga gtacctggcg aactcatgga
     3781 gaaactgaca tcaggacagc gcgccgccac ccgtatgata ctggaaacgt ctgaccgttt
     3841 cacggtggtg cagggctatg ccggtgtggg taagaccaca cagttccggg cggtgatgtc
     3901 agccgtgaac atgctgccgg agagtgaacg tccccgtgtc gtggggctgg ggcccacaca
     3961 ccgtgcggtc ggggagatgc gcagcgccgg cgtggatgca cagacactgg cgtcctttct
     4021 gcatgacacg cagcagcgca gcggagaaac gccggatttc agcaacacgc tgttccttct
     4081 cgatgagagc tctatggtgg gcaataccga catggcacgg gcatacgccc tgattgcggc
     4141 cggtggcggt cgggctgtgg ccagcggtga cacggaccag ctgcaggcca tcgcgcccgg
     4201 tcagcctttc cgtctccagc agacgcgcag tgctgccgat gtggtcatca tgaaggagat
     4261 tgtgcgtcag acgccggaac tgcgggaggc ggtatacagc ctgattaacc gggatgtgga
     4321 aagggcgctg tccgggcttg agcgtgtgaa accgtctcag gtgccacgtc tggagggcgc
     4381 atgggcaccg gagcactccg tgacggagtt cagtcacagc caggaagcga aactggcaga
     4441 agcgcagcag aaggcgatgc tgaaaggcga ggcttttccg gatgtcccca tgacactgta
     4501 tgaagccatt gtccgcgact ataccggcag gacaccggaa gcacgggagc agacgctgat
     4561 tgtcacgcac ctgaatgagg accggcgcgt actgaacagc atgattcatg atgcacggga
     4621 aaaggccgga gaactgggga aggtgcaggt catggtgcct gtcctgaaca cagcgaatat
     4681 acgcgacggg gagctgcgtc gtctctccac ctgggagaat aacccggatg cccttgccct
     4741 ggtggataat gtgtatcacc ggattgccgg tatcagcaag gatgacgggc tgataaccct
     4801 gcaggatgcg gaaggtaaca cgcggctgat ttcgccccgg gaggcggtgg ctgaaggcgt
     4861 cacgctgtac accccggaca ccatccgggt ggggaccggt gacaggatac gcttcacgaa
     4921 gagtgaccgg gagcgcggtt atgtggccaa cagcgtctgg acggtgacag cggtttccgg
     4981 tgacagtgtc acactttctg acggtcagca gacccgtgtg attcgccccg gtcaggagcg
     5041 ggcagagcaa catattgacc tggcctatgc catcaccgcc cacggtgcac agggggcgag
     5101 tgaaaccttt gccatcgcgc ttgaaggcac ggaaggtaac cggaaactga tggccggctt
     5161 tgagtcagcc tacgtggccc tgtcgcgtat gaagcagcat gtgcaggtgt acaccgataa
     5221 ccgtcagggc tggacggatg ccatcaacaa tgccgtacag aaaggaactg cccacgatgt
     5281 gtttgagccg aaaccggacc gggaggtcat gaatgcagag cggctgttca gtacggcgcg
     5341 ggagctgcgg gacgtggcgg caggccgtgc cgttctccgt caggcggggc tggccggggg
     5401 agacagtcct gcacggttta ttgctccggg acgtaaatat ccgcaaccgt atgtggcact
     5461 gccggcgttt gaccgtaacg gcaagtccgc cggtatctgg ctgaacccgc tgaccacgga
     5521 tgacggaaac gggctgcggg gattcagcgg tgaaggtcgg gtaaaaggca gcggggatgc
     5581 gcagttcgtg gccctgcagg gcagccgtaa cggagaaagc ctgctggctg ataatatgca
     5641 ggatggcgtc cggattgccc gtgataatcc tgacagtggt gtggtggtaa gaatcgccgg
     5701 tgaaggccgt ccgtggaatc ccggtgccat caccggtggc cgcgtgtggg gggatatccc
     5761 ggacaacagc gtccagccgg gagccggaaa tggcgaaccg gtcacggcag aggtactggc
     5821 acagcggcag gctgaagagg ccatccgccg tgaaacggaa cgtcgcgcag atgaaattgt
     5881 ccgtaaaatg gcagagaaca aacctgacct gccggacggc aaaacagagc aggctgtcag
     5941 ggaaattgcc ggtcaggagc gtgaccgggc tgccataact gaacgggaag ccgcgctgcc
     6001 ggagagtgta ctgcgtgaac cacaacgggt gcgggaagcg gtccgggaag ttgcccggga
     6061 aaatttgctg caggagcgtc tgcagcagat ggagcgggat atggttcgtg acctgcagaa
     6121 agagaaaacc ccgggcggag actgatacag gaagacaaac actgatgaca acggataaca
     6181 cgaacacgac acgtaacgat tcactggctg cacggaccga tacctggttg cagtcatttc
     6241 tggtctggtc acccggacag cgggatatca tcaaaacggt ggcactggtg ctgatggtac
     6301 tggaccacat caatctgata ttccagctga agcaggaatg gatgtttctg gccgggcgag
     6361 gagcctttcc gctttttgcc ctggtgtggg ggctgaatct gtcccgtcat gcgcatatcc
     6421 ggcaaccggc cattaaccgg ctgtggggat ggggaattat tgcccagttc gcatattatc
     6481 tggcaggctt tccttggtat gaggggaata tcctgtttgc ctttgcagtg gcggcacagg
     6541 tgctgacgtg gtgtgaaacg cgcagtgggt ggcgtacagc agcggccata ctcctgatgg
     6601 ccctgtgggg gcctttgtcc ggcaccagtt acggtattgc cgggttgctg atgctggcag
     6661 tcagcaaccg gctgtaccgg gcggaagaca gagcggaacg tctggcgctg gtcgcctgcc
     6721 tgctggctgt tattccggcg cttaaccttg ccaccagtga tgcagcggcg gtggccggtc
     6781 ttgtgatgac ggtgctgacc gttggtctgg tgttgtgtgc cggaaaatca ttaccccgtt
     6841 tctggcccgg agactttttc ccgacgttct atgcctgtca tctggctgtg ctgggcgttc
     6901 tggcgctgtg acgggtgtgg tatctttggc cgcaagagga tgattcgtca gaggcagaac
     6961 acagcatgat agagcagaag cgaccggtac tgacactgaa gcggaagaca gagggaacag
     7021 cgcctgtccg cagccggaaa accatcatca atgtcaccac gccaccaaag tggaaggtga
     7081 aaaagcagaa gctggccgag aaagcggccc gtgaagcaga gctggcggca aaaaaagcgc
     7141 aggccagaca ggcgctgtcc atttatctga acctgcccac gctggatgag gctgtgaata
     7201 ccctgaagcc ctggtggccg ggattatttg acggtgacac gccccggctt ctggcctgcg
     7261 gtatccggga cgtgttactg gaagacgtgg cgcagcggaa tatcccgctc tcgcataaaa
     7321 aactgcgcag ggcgctgaag gccatcaccc gttcagaaag ctatctgtgt gccatgaaag
     7381 ccggtgcctg ccggtatgac acggaagggt atgtgacgga gcatatttct caggaggagg
     7441 aagcgtatgc gggagcgcgt ctggcgaaaa ttcgtcgcca gaaccggata aaggcagaac
     7501 ttcaggccgt gcttgatgag aaataaaaaa agcctccccc agagagagga ggcagggaaa
     7561 taaggcttaa aaggaaataa cttccgcttc cgaaaggaag ccagtccact gaattattta
     7621 tcacaataat actgacaact aaagggggac aagaaaatga atggattcag aaacagttca
     7681 cggaacggcc aggtctggcg ttaccagcgt gccggcgggc gggccgttat tctggaagtc
     7741 agtggacgct ggatggaagc ggcagaagca tggcgacggg ctgcctgtgt tgcgcctagg
     7801 acagactggc agcagtttgc ccggaaaagg gctgaacact gccaccggcg ctgccgggga
     7861 agggtgtaac gaaaaaagca gccttcatta ctctaggagt cagaaggctg aaaggagttt
     7921 atgagatttg cttcttaata tccggtcggg cagtatgacc ggacagttct gagtagcaca
     7981 gagtcttttc cgcattaagt cgatttttta tattttgtga ttaatttcac aaaataaggt
     8041 gttattcaat gtgtgctgca ctattcaggg ggtctgaaat acaggtggca tttatctgat
     8101 atggagaata acatgaggaa atatattcca ctggtattat ttatcttttc atggccggta
     8161 ttgtctgcag atattcatgg acgggttgtt cgtgtactgg acggtgatac catcgaggtc
     8221 atggactcac tgaaggcggt gcggatcagg ctggtaaata ttgatgcacc ggaaaagaaa
     8281 caggattatg gccggtggtc aacagacatg atgaaatcac tggtcgcagg aaaaacggtt
     8341 accgtcacat a
//
LOCUS       DADMEQ010000143         8320 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.145, whole genome shotgun sequence.
ACCESSION   DADMEQ010000143 DADMEQ010000000
VERSION     DADMEQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.145"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            334..3183
                     /locus_tag="JNZ05_003501"
                     /pseudo
     CDS             334..3183
                     /locus_tag="JNZ05_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3283..5211)
                     /gene="dhaR"
                     /locus_tag="JNZ05_003502"
     CDS             complement(3283..5211)
                     /gene="dhaR"
                     /locus_tag="JNZ05_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HAY8566590.1"
                     /translation="MRDMSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGV
                     TFASIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSA
                     LGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRL
                     TGTIALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDG
                     VISWDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEA
                     TFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDD
                     PQTRRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGD
                     AALAEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITR
                     LDARRLIPIDVKVIATMTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPAL
                     VNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSD
                     LPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTL
                     WRKMKQHGIDAGQFKRRV"
     gene            5430..6500
                     /gene="dhaK"
                     /locus_tag="JNZ05_003503"
     CDS             5430..6500
                     /gene="dhaK"
                     /locus_tag="JNZ05_003503"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAY8566591.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHHTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGR"
     gene            6511..7143
                     /gene="dhaL"
                     /locus_tag="JNZ05_003504"
     CDS             6511..7143
                     /gene="dhaL"
                     /locus_tag="JNZ05_003504"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HAY8566592.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7154..>8320
                     /gene="dhaM"
                     /locus_tag="JNZ05_003505"
     CDS             7154..>8320
                     /gene="dhaM"
                     /locus_tag="JNZ05_003505"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAY8566593.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEE"
BASE COUNT         2174 a         2070 c         2074 g         2002 t
ORIGIN      
        1 cccataacta attaggatta ttacaaaaaa tcttcaccaa attttttaac cgctgtttgt
       61 tgtaatattt ttgccatatt catagtaacg aattggaata agtaacgaca acttagggaa
      121 taactcgata taaactcacc tttgtaaggg gggtgttaaa tatatattaa ttcctgtgtt
      181 cctgattttg cccggtaata gagaattatt ctcagagaag ctcatcatga ttgcaccata
      241 cagatatcat gatgataaga ggatattatt cattgactta aacaaaatga caaaatccat
      301 agacccatag cctgggtact atataggagt ggtatgggca tcaaacaaca caatgggaat
      361 accaaagccg atcgtctcgc tgaattaaaa atccgttcgc cctcaattca actgataaaa
      421 tttggcgcta ttggtttgaa tgcaattatc ttttcccccc tgctgatagc tgctgataca
      481 ggaagtcaat atggcaccaa tattactatt aatgatggtg acagaattac aggagatacc
      541 gccgatccat caggaaacct ctatggtgta atgaccccag caggaaacac gcctggcaat
      601 atcaacctgg gtaatgatgt caccgtcaat gtcaacgacg cctctggata tgcaaaagga
      661 atcattattc agggcaaaaa cagctccctg acagctaacc gactcacagt agatgttgtt
      721 ggtcaaacct ctgccatcgg cattaactta attggtgact atacccatgc tgacttaggc
      781 acaggcagca ccattaagag taacgatgac ggcatcatta ttgggcatag ctcaacacta
      841 acagccactc aattcaccat tgaaaactcg aacggtatag gcctaaccat caatgactat
      901 ggcaccagtg tcgatcttgg aagcggaagt aaaatcaaga ccgatggaag tacaggtgtt
      961 tatatcggtg gtctcaacgg caataacgcc aatggtgctg cgcgttttac ggcgacagac
     1021 ctgacaatcg atgttcaggg ctacagcgcc atggggataa acgtacagaa aaactctgtt
     1081 gtcgatctcg gaacaaacag taccattaaa accaatggcg ataatgctca cggcctctgg
     1141 agctttggcc aggttagcgc gaatgcactc actgttgatg taactggagc cgcggccaat
     1201 ggcgtcgaag ttcgtggtgg tacaaccact atcggtgcag atagccatat ttcttccgcg
     1261 cagggcggtg gcctcgtcac cagtggttca gacgcgacaa tcaatttttc tggcacggca
     1321 gcgcaacgaa acagcatctt ttccggcggt tcttatggtg cctcggccca gacggcaacg
     1381 gctgttatca acatgcaaaa taccgatatt acggttgatc gtaatggcag tctggcgctg
     1441 ggtttgtggg cgctcagcgg cggtagaata accggagaca gtttggctat caccggcgcg
     1501 gcaggagcca gagggattta tgccatgacc aacagccaga tcgacctcac gagcgatctg
     1561 gtcattgata tgagtacacc cgaccagatg gccatcgcaa cgcaacatga cgatggttat
     1621 gccaccagcc gcatcaacgc ctcgggtcgt atgcttatca acggtagcgt tctttccaaa
     1681 ggtgggctaa tcaatctgga tatgcaccct gggtcggttt ggacaggttc ctccctcagc
     1741 gataatgtca atggcgggaa actggacgtt gcaatgaata acagcgtctg gaacgtaaca
     1801 agtaattcta atctcgacac gctggcgctg agccattcaa ctgtcgattt tgccagccac
     1861 gggtcaactg ccggcacatt taccacatta aacgtagaga acctgagcgg taacagtacc
     1921 tttattatgc gtgctgatgt tgttggcgag ggtaatggcg ttaataatag aggggattta
     1981 ttgaatatca gcgggagtag tgctggtaat cacgtattgg ctatccgcaa ccagggcagc
     2041 gaggccacaa cgggaaatga agttctgaca gtggtaaaaa ccactgacgg cgcggcctcg
     2101 ttcagcgcgt cttctcaggt tgagttgggg ggatatctgt acgatgtgcg taaaaatggc
     2161 actaactggg agctttacgc ttccgggaca gttccggaac cgactcctaa tcctgaaccc
     2221 acaccagctc ccgctcagcc tcccatagtc aaccccgatc ctacgcctaa acccaccacg
     2281 accgcagatg ctggcggcaa ttatctcaat gtcggttact tattgaacta tgttgaaaac
     2341 cgtacgctga tgcaacggat gggtgacttg cgaaatcaga gtaaagacgg taatatctgg
     2401 ttgcgcagtt atgggggaag cctggactcc tttgccagtg gcaaactgag cggctttgac
     2461 atgggttaca gcggtatcca gtttggtggg gataaacgtc tctctgatgt aatgccgttg
     2521 tatgtcggtc tgtatattga ctcaacacat gcatcgccgg actatagcgg aggcgacggt
     2581 accgcacgtt cagactacat gggaatgtac gccagttaca tggcacaaaa cggtttttac
     2641 agcgatctcg ttataaaagc atcgcgccag aaaaatagtt tccacgtact ggacagtcag
     2701 aacaactgcg ttaacgccaa cggcactgcg aatggaatga gcatctccct ggaagccggg
     2761 cagaggttca acctgtcccc tactggttat gggttctata tagagccgca aacccagctt
     2821 acatacagcc accagaatga gatggctatg aaggcgagta atggcctcaa tatacatctg
     2881 aatcactacg aatcgctgct ggggcgtgcc agcatgatac tggggtatga catcaccgca
     2941 ggcaacagcc agctgaatgt ctatgtgaag actggcgcta tccgcgagtt ttcaggggat
     3001 accgaatatc tgttgaacga ctcccgggag aagtacagtt tcaaaggtaa tggctggaat
     3061 aacggcgtgg gagtcagtgc acagtataac aaacagcaca cattctatct ctaagcggat
     3121 tacacgcagg gtaacctctt tgatcagaag caagtcaacg gaggatatcg cttcagcttt
     3181 taagtgcggg atagacaagc ccttaaacga tgctgaaaat tgttagcgtg cgccggaatg
     3241 aaatacagat cactctgatg tatcagaaat cgtttctgtc tttcataccc ggcgcttaaa
     3301 ctgccctgca tcaatgccat gttgcttcat tttccgccac agcgtagtgc ggccgatccc
     3361 aagtaaagcc gacatttcct gaatgcgacc gcctgtgacc tgggctgcgt taataattgc
     3421 ctctttttca acttccgcaa atgacagact ggtggaaagg cgggtggcgc tgacatcatc
     3481 tgtcgcctgc tcggtaaaca gatgttcagg taaatcactg acgcgaatgc gcccgttatc
     3541 actactcaga gcaagattct cgatgacgct gtaaagttca aaatcgttgc ctggccatgc
     3601 acaagaaact aggcgagcga gggcatcgtc atcaattttc agccgcgtag agaagcgttt
     3661 ttcaagactg cgtaatttgt tattcaccag cgccggaatg ctgccacgcc gcatacgcag
     3721 aggcgggatg gtaatttcaa atgcatgcag cgcgtaatac agctggcgac taaaacgatt
     3781 ttgttccacc agcattgcga ggtccgcggt cattgttgca atcactttga catcaattgg
     3841 tattaaacgc cgcgcatcca gtcgcgtgat aaccccctgc ttgataacct gcagcaaagc
     3901 agactgtaac tccaccgcca gatattcaat cttttcaaga aacagcgtgc cgccgtgtgc
     3961 cagttccagc cgactcagac ggccattttc attgtccgtg cgatcgccac caataaattc
     4021 ttccgccagc gcagcatcac catataactc acaattgacg gcgatataag gacctgcagc
     4081 acgctcgctt tcattatgaa ttgcctgact tagcagtgcc ttgcccacgc cctcttctcc
     4141 acaaagcagg acaggaaagc tactgcgcgc cgcctggcga ccaaaatgaa tcaagcggcg
     4201 ggtttgcgga tcgtcctgtg gcatatgagc gaaggtatgg ctgacttttc ctaattgact
     4261 ggtcattaac tgccgcatct gttccacagg atggagcaac aaaataaagc tggttccctg
     4321 cgtttctatt atcggtttaa gggttatcac cgcatcaata aactggtgct ggctttcaaa
     4381 ggttgcttct acgtgtttga gcggatgtgc ctgttttatt gcttgttgca atacggcggg
     4441 taacgttaag agttcagtga ttgcccgtcc ctgacttgcc gtcgcgtcaa ggcgcaagac
     4501 ccgcgccgcc tgggcattaa taaattgcaa attaccctgc tcgtcccagc taatcacgcc
     4561 atcatccata ctttctaaca gggcattaag ttgatttaaa tgacggttag tttcagcgag
     4621 caaactgtcc gtcagcagta aattaccgac ctcgcgggcg attgccaacg tcaacggcaa
     4681 atcagctgcg gtagtttgct caaccggaca cgccagcgct attgttcccg tcaatcggcc
     4741 cttgctgtca aacaacggcg ttgcacaaaa ggcccagttc cagagtgcct gtttgaaatg
     4801 ttgatcggcc atcgttttca cggcctgacc agagatagcc gctaacgata gcgcacaagt
     4861 accaataatt ccctcggcgc aatacgtgcc gtcattgaat cccagtgcac ttagctgctg
     4921 caaggtttgc ggatcgccat tacggctgag aatgcaggcg gtttcatcga ggataaacag
     4981 cgcacactct cgcggtgcca tatattccca ggcatcttcc agcgcagcct gcccgagcgt
     5041 cagcatcgct ttcttacgcc gataaataga agcaaatgtc acgccctggg cctgatgtgg
     5101 tacgttccat gtctcccgtt tcatcagctt attgcatcgc tcccaggagg ttgcaattaa
     5161 gggagatatg ccccgaccat cgttgttaaa agcgccactc atatcccgca tcccttatga
     5221 caaaaggtgt tccggctcgc aattttaagt attaatacac gtaaaagttt caatatggaa
     5281 cattcattaa agcatatgtt tcactatgaa acataatttg ggctaatttt cttttccctt
     5341 gcctgatgca caatggattc atcggcagtc agtggtcgcc gtgtcgttga acatcatcca
     5401 tgccctaccg taattgctgg cgcaaaataa tgaaaaaatt gatcaatgat gtgcaagacg
     5461 tactggacga acaactggca ggactggcga aagcgcatcc atcgctgaca ctgcatcagg
     5521 atccggtgta tgtcacccga gctgatgccc ctgttgcagg aaaagtcgcc ctgctgtcgg
     5581 gtggcggcag cggacacgag ccgatgcact gtggttatat cggtcagggg atgctttcgg
     5641 gggcctgtcc gggcgaaatt ttcacctcac cgacgcccga taaaatcttt gaatgctcca
     5701 tgcaaattga tggcggcgaa ggtgtactgt tgattatcaa aaattacacc ggcgatattc
     5761 ttaactttga aacagcgacc gagttactgc acgatagcgg cgtaaaagtg accactgtgg
     5821 tcattgatga cgacgttgcg gtaaaagaca gtctttatac cgccgggcga cgcggcgttg
     5881 ccaacaccgt attaattgaa aaactcgtag gcgcagcggc ggagcgtggc gactcactgg
     5941 acgcctgtgc ggaactgggg cgtaagttga ataatcaagg ccactcaata ggtatcgctc
     6001 tcggtgcctg taccgttcct gccgcgggca aaccttcttt tacccttgcg gataatgaga
     6061 tggagtttgg cgtcggcatt catggtgagc caggtattga ccgccgccct ttctcttccc
     6121 ttgatcaaac cgtcgatgaa atgttcgaca ccctgctgga acatggctca taccatcaca
     6181 ctttgcgttt ctgggattat caacaaggca gctggcagga agaaccacaa accaaacaac
     6241 cgctccagtc tggcgatcgg gtgattgcac tggttaacaa tcttggcgca actccgcttt
     6301 ctgagctgta cggcgtctat aaccgcctga ccacacgttg ccagcaagcg ggattgacta
     6361 tcgaacgtaa tttaattggc gcgtactgca cctcactgga tatgaccggt ttctcaatca
     6421 ccttactgaa agttgatgac gaaacgctgg ctctctggga cgccccggtc cacaccccgg
     6481 cccttaactg gggtagataa ggagaaagca atgtcactga gcagaactca aattgttaac
     6541 tggctcactc gttgtggcga tattttcaac accgagagcg agtatcttac cgggctggat
     6601 cgcgagattg gcgacgctga ccacgggcta aatatgaacc gaggctttag caaagtggtg
     6661 gaaaaactcc ctgctatcgc agataaagat atcggtttca ttctcaaaaa taccggtatg
     6721 acgctgcttt ccagcgtcgg tggtgccagt ggtccgctgt tcggtacctt ctttatccgc
     6781 gccgcacagg cgacccaggc acggcaaagc ctgacactgg aagagcttta tcagatgttc
     6841 cgcgatggcg cggacggcgt aatcagtcgc gggaaagccg aacctggcga taaaaccatg
     6901 tgtgatgtgt gggtgccggt ggtggaatcg ttacgtcagt ccagcgagca aaatctctct
     6961 gttccggtgg cgctcgaagc tgccagtagc atcgccgaat ccgcggcaca aagtacgatt
     7021 acgatgcaag cccgcaaagg ccgcgccagt tatctcggtg aacgcagtat tggtcaccag
     7081 gatcccggcg cgacctcggt gatgtttatg atgcaaatgt tggcgttagc cgcaaaagag
     7141 taaggaattg gtgatggtaa acctggtcat agtttcacat agcagccgac tgggagaagg
     7201 tgtcggtgaa ttagcccgtc agatgttaat gagtgatagt tgtaaaatcg ccattgccgc
     7261 gggaattgac gatccacaaa atcccattgg taccgatgcc gtcaaagtga tggaggccat
     7321 cgaatctgtt gctgatgccg accatgtgct ggtcatgatg gatatgggta gcgcattatt
     7381 gagtgctgaa actgcgctgg aattgctggc tcccgagatc gccgcaaaag tacgtttgtg
     7441 tgctgcgccg ttggtcgaag gtacactggc agcaacggtc agcgcggcct cgggggcgga
     7501 tatcgacaaa gttatctttg acgctatgca tgcgctggaa gccaaacgtg aacaactggg
     7561 tttaccgtcc tccgacactg aaatctctga cacatgtcct ccgtacgatg aagaagcccg
     7621 ttctctggcg gtggtcataa aaaaccgtaa cggcctgcat gtacgtccgg cctcccggct
     7681 ggtttatacc ttatcgacat ttaatgccga tatgttgctg gaaaaaaacg gcaaatgcgt
     7741 cacaccagag agtattaacc agattgcgtt actacaagtt cgctataacg atacgctgcg
     7801 cctgattgcg aaagggccag aagctgaaga ggcactgatc gctttccgtc agctggctga
     7861 agataacttt ggtgaaacgg aggaagtcgc tccacctact ctgcgtcccg ttctgcctgt
     7921 ttcgggtaaa gccttttatt atcaaccagt tttatgtacg gtacaggcaa aatcaaccct
     7981 gaccgcggaa gaagaacaag atcgattacg ccaggctatt gacttcacgt tattagatct
     8041 gatgacgtta acagcgaaag cagaagccag cgggcttgac gatattgccg caatcttttc
     8101 tggtcaccat acactgttag gtgatccgga actgctggcg gcggcaagcg aactccttca
     8161 gcatgaacat tgcacggcag aatatgcctg gcagcaagtt cttaaagaac ttagccagca
     8221 ataccagcaa ctggatgatg aatatctaca agctcgctat attgatgtgg acgatcttct
     8281 gcatcgcacc ctggtccacc tgacccaaac gaaagaagaa
//
LOCUS       DADMEQ010000144         8250 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.146, whole genome shotgun sequence.
ACCESSION   DADMEQ010000144 DADMEQ010000000
VERSION     DADMEQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.146"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(21..2501)
                     /locus_tag="JNZ05_003506"
     CDS             complement(21..2501)
                     /locus_tag="JNZ05_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY8566594.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPLVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(2517..3191)
                     /locus_tag="JNZ05_003507"
     CDS             complement(2517..3191)
                     /locus_tag="JNZ05_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY8566595.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            complement(3272..3814)
                     /locus_tag="JNZ05_003508"
     CDS             complement(3272..3814)
                     /locus_tag="JNZ05_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8566596.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            complement(4107..4388)
                     /locus_tag="JNZ05_003509"
     CDS             complement(4107..4388)
                     /locus_tag="JNZ05_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HAY8566597.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(4650..5759)
                     /gene="apbC"
                     /locus_tag="JNZ05_003510"
     CDS             complement(4650..5759)
                     /gene="apbC"
                     /locus_tag="JNZ05_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HAY8566598.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            5891..7924
                     /gene="metG"
                     /locus_tag="JNZ05_003511"
     CDS             5891..7924
                     /gene="metG"
                     /locus_tag="JNZ05_003511"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAY8566599.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDKYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            8065..>8250
                     /locus_tag="JNZ05_003512"
     CDS             8065..>8250
                     /locus_tag="JNZ05_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HAY8566600.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
BASE COUNT         2217 a         2028 c         1789 g         2216 t
ORIGIN      
        1 tccatagaac cacctgacta ttactggcaa atataatttc tactttcatc aatctcttta
       61 ccgaaggtga ttgtgcatga aagtccttgt tgcttatcaa ttgctacatt aaccaatggc
      121 ggtatttcat tggtgcgaat aaataactgg cttccctggc cgacaacgcc aatattatga
      181 ccatggatat cattgacttc ataaccaaac gttaatggtt gaccatccgc tcttaacgct
      241 tttataaacc agggcttgcg ctgatcggta tcaaaattaa ccagtacaac cgcgccgcga
      301 taaggggcgg caattttccg gttgccacgt aattctgctt cgctatcgct ttgcgacaca
      361 tccagcatca ggtgattttc ccgataaggt gtcattccgt cgtataccac cactccatta
      421 cggtttgttg tgcgatattt ttgcccattg acataagcat ctttaattcc tggcgcattc
      481 atcacagcaa acgtttcgga aagacggttc gccagattaa cgccacccga ccaggcgaca
      541 atgccccctg aaacactggc tccagtctgt cgataagtac tcgactgact ataactgcca
      601 ttcactgtcg caaccggcgc gttccaggtc aaattcgccc cagctgtcgt ttcatttccc
      661 tgatgttgat gactcaggtt gacaccataa ttgaactgat cccgactccc tactgttcct
      721 gataatcccg tattatttga ggcaaaaccc tgatcatcaa acgtcgttga gttagacata
      781 tatatttgcc gacgaggcgt cgtaacgtca tcaccccaat cacagggaat tgatataaaa
      841 atattaaaac gtttctcttc atgatgattc tcgccatacg cctggcttgc cgcgagggta
      901 tagcttatcc gccgccagtt attggaataa ctcaactgat aatccttact gctgccgcta
      961 cgcccccagt aatctcgcca taacgtactt aaggacacag aaccccagcc ttctggcaat
     1021 gactggctca tattggcaga aaagctattt ttacggccaa aatcgttctg gtaataatcg
     1081 gcaatgtcat agatatcgtt ttcatcacgg cgataattat ctttattgtt tgcccaaacg
     1141 tgatcgttaa atgtccggta atcacgcgac gaataacgcc aggccgccag accaaaacgc
     1201 gtcgacgttt ggctcacaaa tttgttgtag gcaatttgat aactttgccc gtcaaacaca
     1261 tcgccgttgt cttgtttact atgcgacttc gtggcatcga cggaaattgc gccaatgcgc
     1321 gtgttccaac ccgttccgag ggtaaacgca tagtaattat tagcaaccat cgtgccgcca
     1381 tacagcgtca ataagttatt aaaaccatac tgataacctg cctggacaaa atcactttgc
     1441 ttgctcgccc cttcaatatg gctacggccc gccgcaaaat catatttcga cacgccgggt
     1501 tgcagcatat ttggcaccgc tgcataaggc accagatagg tggttaccga gccgtccgcc
     1561 tctttcacgc taacatcaag atctgctcca ccaccggcca actgcaaatc tgtaatcgca
     1621 aacgggccag gaggaacctc tttctgataa accacaaaac cattctgttc aatagttacc
     1681 agcgcgttac tctgagcaat cccctgcacc cgtggcgtaa aattttgttt cgagttaggc
     1741 aacatctgca tatcacgaaa caaccgcaca ccgctgaagc gaacagaatc aaaaatatcg
     1801 cttgatgtgt acatatcacc cacgcgaagc gtgccgagaa attgggcaaa tccacgttcc
     1861 agatacaggg tattgctttt ccacacccct ggattgttat ttgttttact gaaactggca
     1921 tcagaatgca gttgccactc cagtaaattt aacccgctgt taaaacgtac atatgtactc
     1981 ttgttgttac ccgacgcttt atagtcgctg taatactgac tcacataata agaagtataa
     2041 aacgcattaa taccccgctc ccagttttcc ggtggaacat agccactttc cagttcttcc
     2101 acccaagcct gcgggacact gaaatcgaga cgaaaaaccc cgatatccca gctatagctc
     2161 ccaccctgaa caagttgctc aaatgttaaa cattgcttac cgctggcgaa gttatcgcta
     2221 ttaatgccta accgcttgat aacttctctt gataaacatg tttcttgcgg gttgtcttta
     2281 acaataatct catatttccc gcgccattgc ttattgacat aaatatcgat gtcatactgc
     2341 cccggtaagg gttgattatc atcaagacga atatttgcaa cctgctggtc tttcattcca
     2401 cctatcataa aatgggtatc aaaggtttct tcagctgcat aagcttcaat gccgagcaat
     2461 aacgctacga ttgctgatgc aagtggggtc attctcaaca taggcagctc ctgcaattaa
     2521 attttgtcac taatatagtt gccgtggtca ttgataatgg tcagatacca gttatttgca
     2581 ttattacttt tgatattaac actctgactt tctaaggggg caatcataat agtttcataa
     2641 ttgactttaa cattattagc tttgacatcc gaaatcgtca cccaattagc tgagtcattt
     2701 tttatcacca atccattgcc actgcgattt accagtaact ttttaaaggt cgctttattt
     2761 accggagcaa tacccgctgg ccggtaaaac aacttaattc tgttttgcat cgcaaacttc
     2821 agtgcattct tgccttcttg ctctggacta tttggtggaa tatccagaac attcagataa
     2881 aaaatgcttt ctttattagt gggcagttta ttcggcataa ttttgatttt tacttgctgc
     2941 ccggaatttg cccctatttt tgccactggt ggcgttaaca tgaaaggaac ctgaattttt
     3001 tctggtggta acgacgtatc gccatcatca atccacgcct gcagcagcga agaacgattt
     3061 ccctggttca tcaactgcac cataacttct ttattttctg ccgggtaaat aatgcgcgtc
     3121 ccgtagataa caattccggc atgtgcagga aggaccatag aaaaaatgag taaagataat
     3181 aatcctttca tcttaatgcc tcttaaatta aaaagccgcc atggggatag cggccatgtt
     3241 tattacctgg agaaacttgc aattttattt attaatcctg cgctacacgc ataaccagag
     3301 aagataacac atcacctgat tgcactgagt tatctgtcag tttcaggtat tttgctttaa
     3361 acggaatagc atattgacct tctgctgttt tataactctc aatgttaaaa ggtgcagaaa
     3421 catctaggac atcgctgtta tcacctaaat agagatagta acccacggta tcgttgctac
     3481 tggctatcgc cttatcgtta ccggttattt tgtcgtttgt gatcaacgag agttttacac
     3541 cagcaacttc tttcgggcag ctaattttta acatgctact ggttacaccg actgcatcat
     3601 cataggtaga atatttagct ttaccttcaa agacagtggt tggtacagtc ggcatattaa
     3661 tacttactga atcaccaccc gcttcgaatt tacacggaga ttctgcaata tttcctttaa
     3721 tggttaatgt tccggtatca acgtctgcag caaatactcc agcgctcata aaaaaagaag
     3781 aaaagacagc ggcagcaata attgaacgtt tcataatatg ttccttaata aaatttattt
     3841 ttggttgtta agagtctgta tctcttggaa acaaatttct cacatcaaaa aagctgccgc
     3901 aatgtaaaca tattgtttta ttgatttaaa acaaattaac caatgcaata taaatacaac
     3961 aaatacttaa gaatattaaa tatttatcac atctattaat taatgtagat ttatgtaaat
     4021 ggtattgttg atatcaataa aaaagacgaa atcatttata taaatgattt cgtctttcta
     4081 aatgcaatta aaatcaggtt gccaaattaa tcgtagcgat ttaacacgat tcgacgtttg
     4141 ctatcgctac ctgcaacaac cacttctgtc gtaacacctt tgacgggcga agaaacctga
     4201 tgacgggtgt caacattata cgttaactga ccacaatgct gttgtggttg actattaacc
     4261 atagccatac tgcatgttgg gggactgatc ctgccattaa tcgttaatgt ggcagtctct
     4321 gaagagactg cgggagaagc caatccatag gcaagaaaaa taattcctga cagccaatac
     4381 ttattcattg aacgttatcc ctgtagtaaa ggttatgcct ggatagaatg agtgcataac
     4441 aaactatagc tgtacatcca ctacacagca cgaaggatga taatgaagca ttgcctgtac
     4501 gatcactcga ctttgtagag tttcggacga aggtccgcag aatattcgca gtattaaata
     4561 agtgttcatt aatgcggcaa aaacaaatac accgcatcaa tataacatct ctgcggtgta
     4621 tgaagaataa ccagaatggt taataggcgt tagaccgcgc ggaaggaaat ctcgccagga
     4681 atgacttcac cctgccagta gagctgagct gcaacgcggt cagccagctg gcggtagata
     4741 gcggtaaatt cgctctccgg acggctgata accgttggcg tgcctttatc gagatcctca
     4801 cgtaaggaga tgtgtaacgg catctgcccc agcagctgtg tatggtattt ttcagccagt
     4861 ttctctgcac caccggtacc gaaaatcggt tcatggtgac cacagttact gcaaatatgc
     4921 acactcatgt tctcgacaat accgagcacc ggaacctcga ctttctcgaa catcacaatg
     4981 ccttttttcg catcgatcaa cgcgatgtcc tgcggggtgg tgaccacgac tgcaccagtt
     5041 actggaatgt tttgcgccag cgtcaattga atgtcaccgg tgcctggcgg catatcaagc
     5101 accagatagt cgagatccgg ccacaaggtt tcctgcaaca tctgcattaa cgccttgctc
     5161 gccatcggtc cacgccacac catcgcattg tcgtcagtga ccagataacc aattgaattg
     5221 gttgccaggc catgagacat aatcggtgcc atgtgggtac cgtccggcga agttggacgc
     5281 tgattttctg cgcccagcat ggtgggaatt gatgggccgt agatatcggc gtccagaatg
     5341 ccgactttcg ccccttccgc cgccagtgcc agcgccaggt ttaccgccgt ggacgattta
     5401 cccacgccgc ctttaccgga actcaccgcg ataatgttct tcacaccgtt aattcccggc
     5461 tgattcttca cgcgtttcag agtggctatg ttatgcgaca gtttccagtc gatagctttc
     5521 gccccggtga tgcgcagcag ttcggcgcta cattgctctt ttaactcttc gaacgcgcta
     5581 tgccagacaa acggcataac cagttccaca tgcaacgtgt cgtccatcca ggcgacatgg
     5641 tgcaacgctt tcagcgtggt gaggttatgc ttcagggttg ggtgctgaaa attggccagt
     5701 gtcccggcga ccattgcgcg caaggcttct ggtgatttgg cctgggattg ttcgttcatc
     5761 ccgactcctt ttttgtatag ataaaccatc agctgatagt ttacctgaag aatatagaga
     5821 agtacttact taacattttc ccatttggta ctatctaacc ccttttcact attaagaagt
     5881 aatgcctact atgactcaag tcgcgaagaa aattctggtg acgtgcgcac tgccgtacgc
     5941 taacggctca atccacctcg gccatatgct ggagcacatc caggctgatg tctgggtccg
     6001 ttaccagcga atgcgcggcc acgaggtcaa cttcatctgc gccgacgatg cccacggtac
     6061 accgatcatg ctgaaagctc agcagctcgg tatcaccccg gagcagatga ttggcgaaat
     6121 gagtcaggag catcagactg atttcgcagg ctttaacatc agctatgaca actatcactc
     6181 gacgcacagc gaagagaacc gccagttgtc agaacttatc tactctcgcc tgaaagaaaa
     6241 cggttttatt aaaaaccgca ccatctctca gctgtacgat ccggaaaaag gcatgttcct
     6301 gccggaccgt tttgtgaaag gcacctgccc gaaatgtaaa tccccggatc aatacggcga
     6361 taactgcgaa gtctgcggcg cgacctacag cccgactgaa ttgatcgagc cgaaatcggt
     6421 ggtttctggt gctacaccag tgatgcgtga ttctgaacac ttcttctttg atctgccctc
     6481 tttcagcgaa atgctgcagg catggacccg cagcggtgcg ttgcaggagc aggtggcaaa
     6541 caaaatgcag gagtggtttg aatccggcct gcaacagtgg gatatctccc gcgacgcgcc
     6601 atactttggt tttgaaattc cgaacgcgcc gggcaaatat ttctacgtct ggctggacgc
     6661 accgattggc tacatgggtt ctttcaagaa tctgtgcgac aagcgcggcg acagcgtaag
     6721 cttcgataaa tactggaaga aagactccac cgccgagctg taccacttca tcggtaaaga
     6781 tatcgtttac ttccacagcc tgttctggcc tgccatgctg gaaggcagca acttccgcaa
     6841 gccgaccaac ctgtttgttc atggctatgt gacggtgaac ggcgcgaaga tgtccaagtc
     6901 tcgcggcacc tttattaaag ccagcacctg gctgaatcat tttgacgctg acagcctgcg
     6961 ttactactac actgcgaaac tctcttcgcg cattgatgat atcgatctca acctggaaga
     7021 tttcgttcag cgtgtgaatg ccgatatcgt gaacaaagtg gttaacctgg cctcccgtaa
     7081 tgcgggcttt atcaacaagc gttttgacgg tgtgctggca agcgagctgg ctgaccctga
     7141 gctgtacaaa acctttaccg atgccgctga agtgattggt gaagcgtggg aaagccgtga
     7201 atttggtaaa gcagtgcgcg aaatcatggc actggctgac ctggctaacc gctatgtcga
     7261 cgagcaggct ccgtgggtag tcgcaaaaca ggaaggtcgc gatgctgacc tgcaggcgat
     7321 ttgctcaatg ggcatcaacc tgttccgcgt gctgatgact tacctgaagc cggtactgcc
     7381 gaaactgacc gagcgtgcag aagccttcct caatacggaa ctgacctggg atggtatcca
     7441 gcaaccgctg ctgggccaca aagtgaatcc gttcaaggcg ctgtataacc gcatcgatat
     7501 gaaacaggtt gaagctctgg tggaagcctc taaagaagaa gtgaaagcca ctaccgcgcc
     7561 agttactggc ccactggcag atgatccgat tcaggaaacc atcacctttg acgacttcgc
     7621 caaagttgac ctgcgcgtgg cgctgatcga aaacgcagag tttgttgaag gttctgacaa
     7681 actgctgcgc ctgacgctgg atctcggcgg tgaaaaacgc aatgtcttct ccggtattcg
     7741 ttctgcttac ccggatccgc aggcactgat tggtcgtcac accattatgg tggctaacct
     7801 ggcaccacgt aaaatgcgct tcggtatctc tgaaggcatg gtgatggctg ccggtcctgg
     7861 cgggaaagat attttcctgc taagcccgga tgccggtgct aaaccgggtc atcaggtgaa
     7921 ataatccccc ttcaaggcgc tgcatcgaca gcgccttttc tttataaatt cctaaagttg
     7981 ttttcttgcg attttgtctc tctctaaccc gtataaatac tggtagcatc tgcattcaac
     8041 tggatgaaat tacagggatg cagaatgaga cactttatct atcaggacga aaaatcacat
     8101 aaattctgga cggtggagca acagggaaac gagttgcata tcagttgggg aaaagttggc
     8161 accaaagggc aaagtcagat aaaaagtttt tcagatgctg cggcggcgga aaaagcggaa
     8221 ctgaagctga ttgcggagaa ggtgaagaag
//
LOCUS       DADMEQ010000145         8201 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.147, whole genome shotgun sequence.
ACCESSION   DADMEQ010000145 DADMEQ010000000
VERSION     DADMEQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8201
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.147"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            59..460
                     /locus_tag="JNZ05_003513"
     CDS             59..460
                     /locus_tag="JNZ05_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY8566601.1"
                     /translation="MSSNFCERLEQWGNKSVNNGEERAIAVERIKEAYNSNMASLDLS
                     YLDLSELPPIPSTVNTLNLENNCLTCLDLADIASLVNINLSVNKINTITFPKEPKLEN
                     IYIDHNNLESLDLKNQHSLVNLEAQNNSLKK"
     gene            570..1901
                     /locus_tag="JNZ05_003514"
     CDS             570..1901
                     /locus_tag="JNZ05_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY8566602.1"
                     /translation="MITDLILHNHPKMKTITLNDNHIAHLNAKNTTELEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQ
                     LMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYTIKKAYTPYNIHTYKTE
                     EVVNQRNIKVKNMTLDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            2225..3613
                     /locus_tag="JNZ05_003515"
                     /pseudo
     CDS             2225..3613
                     /locus_tag="JNZ05_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
     gene            3695..7328
                     /locus_tag="JNZ05_003516"
                     /pseudo
     CDS             3695..7328
                     /locus_tag="JNZ05_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
     gene            7325..>8201
                     /gene="narH"
                     /locus_tag="JNZ05_003517"
     CDS             7325..>8201
                     /gene="narH"
                     /locus_tag="JNZ05_003517"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY8566603.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKYIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEAASTEREVDL"
BASE COUNT         2192 a         2030 c         1910 g         2069 t
ORIGIN      
        1 ttgtgtaaaa aataatgcca gttatcaact taataacgca ttacctaaca aagaaaccat
       61 ctccagcaat ttttgtgaac gactagaaca atggggtaat aaatcggtta ataatggtga
      121 agaaagagca attgccgtgg agcgaattaa agaagcttac aattcgaaca tggcatctct
      181 tgatttgtca tatcttgatt tgagtgaatt accgcctatc ccctcgacag ttaatacgtt
      241 aaatctggaa aacaattgtc taacttgtct tgacttagct gacattgcca gtctcgttaa
      301 tatcaacctc agcgttaata aaattaacac gataaccttt ccaaaagaac caaaactgga
      361 aaacatttat attgatcata acaatctgga aagtttagat ttaaaaaatc agcattcatt
      421 ggttaatctg gaagcgcaaa ataacagcct aaaaaaatga tatttctgat agttataaac
      481 tgaaatttct taatcttgat tataacaagc tagcatcgct ggatgtctcc cggcaagaat
      541 ctctgattga gttaagtgcc catcacaata tgatcactga ccttatatta cacaatcatc
      601 ccaagatgaa aacaatcact ttaaacgaca accatattgc acatttaaac gccaaaaaca
      661 ctacagaact ggaatattta aacttaagca ataacaattt actgccaacc aatgacattg
      721 atcaactaat atcatcaaaa catctttggc atgtattagt taacggcatc aacaatgatc
      781 cacttgcaca aatgcagtac tggactgcag taagaaatat aattgatgac actaatgaag
      841 tgaccattga tttatcagga cttaatttaa ccactcaacc accagggctg caaaacttca
      901 cctctatcaa tcttgataat aaccaactca cacattttga tgcaaccaac tacgatagac
      961 tcgtaaagct aagtctgaat agtaatgctc ttgagtcaat aaattttcct caaggcagaa
     1021 atgtaagtat tacacatata tctatgaata ataatgctct cagaaatatt gatatagata
     1081 ggctttcatc agttacttat tttagtgcgg cacataatca actagagttt gtgcaattag
     1141 aatcttgcga atggctgcaa tacctgaatc tcagccataa tcaattaact gatattgttg
     1201 caggtaataa aaatgaactc ttactgctgg atctatccca taataaacta acaagtttac
     1261 acaatgtctt atttcccaac ttgaatacgt tacttattaa caacaatttg ctttctgaaa
     1321 ttaaaatatt ctatagcaac ttccgcaatg ttcagacatt aaacgctgct aacaaccagt
     1381 tgaaatatat aaatcttgat ttcctgactt atcttccatc tatcaaaagt ttaagactgg
     1441 acaataataa aataacccac attgatacta ataatacatc cgatattgga actttattcc
     1501 ccataataaa acagagcaaa aacttaaatt ttttaaatgt ttctgggaag aacaattgcc
     1561 ctactatgca gctcatgtta tttaatttgt tttccccagc acttaagctt aatactggcc
     1621 tggcaattct ttcgcctggt gcatttgaag ttcactctga cggaatagat gtggataacg
     1681 aattgtttca ctatactatt aaaaaagcat ataccccata taatatacac acttacaaga
     1741 cagaggaagt tgtaaaccag aggaatataa aagttaaaaa catgacctta gatgaaataa
     1801 acaatactta ctataataac gattattaca atcaggcaat aagagaggaa ccgatagact
     1861 ttctggacag atcgttttcc tccaattcat ggccttttta gtgaataaat catcactaat
     1921 tctataagtg tctggtcttg ttgagaatat agttaatatg taacccttta gattcacgat
     1981 ctgaagggtt gaaacatgac atacgttcaa agattaaccc ccgtttgaag gttagcttgt
     2041 tagttacatt tagtaacaca tatctggcta cttaatcccc attctgcccc cgcggttacg
     2101 ctttcttatc tattctttca atcagcactt cacccaattc atccttaatt ctaataactg
     2161 gctattgcgc cttacctcct gttttgtctt ttttcattac caatgtgtgc atgtgaggaa
     2221 caatatggca ctgcaaaatg agaaaaatag tcgttatctt ttacgcgact ggaaaccaga
     2281 aaatccggcc ttctgggaaa ataaaggaaa acatattgct cgaagaaacc tctggatatc
     2341 agtcagttgt ctacttcttg ccttctgtat ctagatgcta tttagcgcag ttaccgttaa
     2401 tctcaataaa atcggtttta attttactac cgatcaactc tttttattaa ccgcattacc
     2461 ctccgtttct ggcgcattat tgcgtgttcc ctactccttt atggtgccta tattcggtgg
     2521 acgccaatgg ccggttttta gtactgcaat cctgattatt ccttgcgtct ggctcggaat
     2581 tgccgtgcaa aatccgaata ctccttttgg gatatttatc gttatcgctt tgctatgcgg
     2641 ttttgcaggt gcaaactttg cttcgagcat gggcaatatc agtttcttct ttccaaaagc
     2701 caaacaaggg agcgctcttg ggattaatgg cggattagga aacttaggtg taagtgtaat
     2761 gcagctggtt gcaccgctgg tcatttttgt acctgtattt gcctttctcg gcgtcaatgg
     2821 cgtaccgcag gccgacggtt cggtgatgtc gctggcgaat gccgcatgga tttgggtacc
     2881 gctactggcg attgccacga tcgccgcctg gtcagggatg aatgatatcg ccagttcacg
     2941 cgcctcaatt gccgaccagc tccctgtctt acaacgcctg catctctggc tgctgagcct
     3001 gctttacctt gccaccttcg gttcgtttat cggtttttct gcgggttttg ccatgctggc
     3061 aaaaacccag ttcccggatg tgaatattct gcgcctggcg ttctttggcc catttattgg
     3121 tgccatcgcg cggtcggttg gtggtgctat ttccgataaa ttcggcggcg tgcgggtgac
     3181 gttgatcaac tttattttta tggcgatttt cagtgccctg ctgttcctta ccttaccggg
     3241 cacaggctcc ggtaatttca tcgcctttta cgccgtattt atggggctgt ttctgaccgc
     3301 gggtctggga agtggttcta ctttccagat gatcgccgtc atctttcgcc agataaccat
     3361 ttatcgggtg aagatgaaag gcggtagtga tgagcaagct cagagagaag ccgtcaccga
     3421 aacggcggca gcgctgggct ttatctcagc cattggcgca gtgggcggct tttttattcc
     3481 gcaggcattt ggcatgtcgc tcaatatgac cggctctccg gtcggcgcga tgaaagtgtt
     3541 tttaatcttc tacatcgttt gtgtgctgct gacctggctg gtttatggtc ggcggaagtt
     3601 cagccaaaaa taagagatcg atgcctgatg tacaaccgtg tcgcatccga caaggcatca
     3661 gatcccaatt cattcctgga gcaggagtca tgtcatgagt aaacttttgg atcgctttcg
     3721 ctacttcaaa caaaagggcg aaacctttgc cgatggtcac ggacaggtga tgcatagcaa
     3781 ccgcgactgg gaggacagct atcgccagcg ttggcagttc gacaaaatcg tgcgttccac
     3841 ccacggcgtt aactgtacag gctcctgtag ctggaaaatc tacgttaaaa atggtctggt
     3901 gacctgggaa atccaacaga ccgactaccc gcgcactcgc cctgacctgc ccaatcatga
     3961 acctcgcggc tgcccgcgtg gcgcaagtta ctcctggtat ctttacagcg ctaaccgcct
     4021 gaaatacccg ctcattcgta aacgactgat tgaactgtgg cgcgaagccc tcaaacaaca
     4081 cagcgatccg gtactggcgt gggcatcgat tatgaacgat ccgcaaaagt gcctgagcta
     4141 caaacaagtg cgtgggcgcg gcgggtttat ccgctccaac tggcaggaac taaaccagct
     4201 gattgccgcc gctaacgtct ggaccatcaa aacctacggc ccggatcgcg ttgccggttt
     4261 ctcgccgatc ccggcgatgt cgatggtttc ttacgccgcc ggaacgcgtt atctgtcgct
     4321 gcttggcggc acctgtttaa gtttctacga ctggtattgc gacctgccgc ccgcctcgcc
     4381 gatgacctgg ggcgagcaaa ccgacgtacc ggaatctgcc gactggtata actccagcta
     4441 catcatcgct tgggggtcta acgtaccgca gacacgtacg ccggacgccc acttctttac
     4501 cgaagtacgc tacaaaggca ctaaaaccat cgccattacc cctgactact ctgaagtggc
     4561 caaattgtgc gaccagtggc tggcaccgaa acaaggcact gatagcgccc tggcgatggc
     4621 aatggggcat gtgattttaa aagagtttca tctcgataat cccagcgact actttatcaa
     4681 ctactgccgc cgctacagcg acatgccgat gctggtaatg ctggagcctc gcgacgatgg
     4741 tagctacgtt cccgggcgca tgatccgcgc atctgacctg gtggatggac tgggcgaaag
     4801 caacaatccg cagtggaaaa ccgtagcagt taataccgca ggtgaattgg tagtgccgaa
     4861 cggttcgatt ggtttccgct ggggagaaaa aggcaaatgg aatctggaat ccattgccgc
     4921 cggtacggaa accgaattgt cgttaaccct gctcggtcaa catgacgctg ttgcaggcgt
     4981 ggccttcccc tactttggcg gcattgaaaa tccgcatttt cgcagcgtaa aacacaatcc
     5041 ggtgctggtg cgccaattgc ccgttaaaaa cctgacgtta gtcgatggca acacctgtcc
     5101 agtggtcagc gtttatgatt tggtactggc gaattacggc ctcgatcgcg ggctggaaga
     5161 tgaaaacagc gcgaaagatt acgctgaaat caaaccgtac accccagcct ggggtgagca
     5221 aattaccggc gtaccgcgcc agtatattga aaccatcgcc cgcgaatttg ccgataccgc
     5281 ccataaaacg catgggcgct caatgattat cctcggcgca ggtgttaacc actggtatca
     5341 catggacatg aactaccgcg ggatgatcaa tatgctgatc ttctgcggtt gtgtcgggca
     5401 aagcggtggc ggctgggcac actatgtcgg ccaggaaaaa ctgcgcccac aaaccggctg
     5461 gttgccactg gcctttgcgc tcgactggaa ccgaccaccg cgccagatga acagcacctc
     5521 gtttttctac aatcattcca gccaatggcg ctatgaaaaa gtctctgctc aggagttact
     5581 ttcaccgctc gccgatgcca gtaagtacag cggtcatctg attgatttca acgttcgcgc
     5641 cgaacgtatg ggctggctac cttctgcgcc gcagctgggg cgtaacccgc tcgggattaa
     5701 agctgaagcc gacaaggccg gattatcccc cacagaattt accgcccagg cgctgaaatc
     5761 gggcgattta cgtatggcct gcgaacaacc agatagcggc agcaatcatc cgcgtaattt
     5821 gtttgtctgg cgctctaacc tgcttggctc ctccggcaaa ggccacgagt atatgcagaa
     5881 gtatctgctg gggaccgaaa gcgggattca gggcgaggaa ctcggtgcca gcgacgggat
     5941 caaaccggaa gaagtcgagt ggcaaactgc ggcgattgaa ggcaagctcg acctgctggt
     6001 gacgctcgac ttccgcatgt ccagtacctg cctgttctcc gatatcgtcc tgcccaccgc
     6061 cacctggtat gaaaaagacg atatgaacac ctcggatatg catccattta ttcatccgct
     6121 ttctgcggcg gtcgatccgg cctgggaatc acgcagcgac tgggaaatct acaaaggtat
     6181 tgccaaagca ttttcgcaag tgtgcgtggg ccatcttggc aaagaaaccg acgtggtatt
     6241 acaaccactg ctgcatgact ctccggcaga gctctcacag ccgtgtgaag tgctcgactg
     6301 gcgcaaaggc gaatgcgatc tgatcccggg taaaaccgcg ccgaatattg tggcggtgga
     6361 gcgcgactac cctgctacgt atgaacgctt tacctcgctc gggccattga tggacaaact
     6421 tggcaatggc ggtaaaggga tttcgtggaa tacccaggat gaaatcgatt tcctcggcaa
     6481 actcaattac accaagcgtg atggcccggc acaggggcgt ccgctgattg acaccgccat
     6541 tgacgcttca gaagtgattc tggcactggc accggaaacc aacggtcatg ttgcagtcaa
     6601 agcgtggcag gcgctgggcg agatcaccgg gcgcgaacat acccatctgg cgctgcacaa
     6661 agaggacgag aagattcgct ttcgcgatat tcaggcgcag ccgcgtaaaa ttatctccag
     6721 ccccacatgg tccggtctgg aaagcgatca cgtatcctat aacgcgggat acaccaacgt
     6781 tcatgagtta attccgtggc gcacgctgtc gggacgccag cagctctatc aggatcatcc
     6841 gtggatgcgt gcttttggtg aaagcctggt ggcttatcgc ccgcctatcg acacccgtag
     6901 cgtcagtgag atgcgccaga taccgccaaa cggcttccag gaaaaagcac ttaacttcct
     6961 gacgccgcac cagaaatggg gcattcactc aacctacagt gaaaacctgc taatgctgac
     7021 gctctctcgc ggtggtcagc caacgtgtac cgccgggcat gaccatgatg tatcacgccc
     7081 aggaacgcat tatgaatatt cctggttcgg aagtaactgg catgcgcggc ggcattcata
     7141 acttggtcac ccgcgtttgc ccgaaaccga cgcatatgat tggcggttac gcgcagctgg
     7201 cctggggctt taactactac ggcaccgtcg gctcgaaccg cgacgaattc atcatgatcc
     7261 gcaagatgaa gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga
     7321 aaaaatgaaa atacgttcgc aagtcggcat ggtgcttaac ctcgataaat gtatcggctg
     7381 ccatacctgt tcggtgacct gtaaaaacgt ctggaccgga cgcgaaggca tggagtacgc
     7441 atggtttaac aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca
     7501 ggaagagtgg caaggcggct gggtccgcga tgtgaatggc aagatccgtc cgcgtctggg
     7561 cagcaagatg ggcgtaataa ccaaaatctt cgccaacccg gtggtgccgc agcagattga
     7621 tgattactac gaacctttca ccttcgacta cgaacattta catagcacac cggaaggcaa
     7681 acatatccct actgctcgcc cgcgttcact gattgacggc aaacggatgg acaaagtgat
     7741 ctggggccca aactgggaag aactgctggg cggcgagttt gaaaaacgtg cccgcgaccg
     7801 caacttcgag gccatgcaaa aggagatgta cgggcagttt gaaaacacct tcatgatgta
     7861 cctgccgcgc ctgtgcgaac actgcctcaa tcccagctgc gtggcgacct gcccaagcgg
     7921 cgctatctac aaacgcgaag aagacggcat tgtgctgatt gatcaggata aatgccgtgg
     7981 ctggcgtttg tgcattagcg gttgtccgta caaaaaaatc tacttcaact ggaaaagcgg
     8041 caagtcagaa aaatatatct tctgttatcc gcgaattgag tcaggccaac ccaccgtgtg
     8101 ctcagaaacc tgcgtgggtc gcatccggta tctgggcgtg ctgctttacg acgccgaccg
     8161 catcgaggaa gcggcgagca ccgagcgcga agttgacctc t
//
LOCUS       DADMEQ010000146         8195 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.148, whole genome shotgun sequence.
ACCESSION   DADMEQ010000146 DADMEQ010000000
VERSION     DADMEQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.148"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..671)
                     /gene="chbR"
                     /locus_tag="JNZ05_003518"
     CDS             complement(<1..671)
                     /gene="chbR"
                     /locus_tag="JNZ05_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HAY8566604.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTDSISVLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIIN"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="JNZ05_003519"
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="JNZ05_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HAY8566605.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1088..2438)
                     /gene="chbC"
                     /locus_tag="JNZ05_003520"
                     /pseudo
     CDS             complement(1088..2438)
                     /gene="chbC"
                     /locus_tag="JNZ05_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2523..2843)
                     /gene="chbB"
                     /locus_tag="JNZ05_003521"
     CDS             complement(2523..2843)
                     /gene="chbB"
                     /locus_tag="JNZ05_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HAY8566606.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIGSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3142..3480)
                     /gene="osmE"
                     /locus_tag="JNZ05_003522"
     CDS             complement(3142..3480)
                     /gene="osmE"
                     /locus_tag="JNZ05_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HAY8566607.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3682..4509
                     /gene="nadE"
                     /locus_tag="JNZ05_003523"
     CDS             3682..4509
                     /gene="nadE"
                     /locus_tag="JNZ05_003523"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAY8566608.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4739..5626
                     /gene="cho"
                     /locus_tag="JNZ05_003524"
     CDS             4739..5626
                     /gene="cho"
                     /locus_tag="JNZ05_003524"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HAY8566609.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5586..6161)
                     /gene="ves"
                     /locus_tag="JNZ05_003525"
     CDS             complement(5586..6161)
                     /gene="ves"
                     /locus_tag="JNZ05_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HAY8566610.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLWLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6364..6849)
                     /gene="spy"
                     /locus_tag="JNZ05_003526"
     CDS             complement(6364..6849)
                     /gene="spy"
                     /locus_tag="JNZ05_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HAY8566611.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7179..8147)
                     /gene="astE"
                     /locus_tag="JNZ05_003527"
     CDS             complement(7179..8147)
                     /gene="astE"
                     /locus_tag="JNZ05_003527"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HAY8566612.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHSEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         2152 a         2056 c         1891 g         2096 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactgcg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtac gctgatacta tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttcca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 tcagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcaatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagt
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgcgctttac gctcttctgc cagcgccatg ccaataaaga acggtgccat taaagacatt
     2161 attcctaatg ttccgttata tacgttgccg ccaatacctt tcagaccatt aagtgtttca
     2221 atggttgagg cgtcgaggcg aatacctaag gaataaaaaa acgacccctc cccaaagctt
     2281 agaaaaacgt tgttaattaa tacaaacatg gcccccgcaa gggttaacgg cattaagcga
     2341 ataaagccat ttttgattgc attaacgtgt ggctgctttc ctattttaac tgcaaaaggg
     2401 aggagtacct tttcaagcga tgcaataaca ttactcatag aaaaataccc ttaaaaaccg
     2461 caatttaaat attgcggttt tgatttatga aataactctt tgacgggaaa atttaaaata
     2521 aattaatttg ctgcggcttt tttaatcgct gcaaccgcag ccttaagcac gcctaaacca
     2581 tcgactttgc cataaagcag cgagccaatt acttcaaccg gtttgttggg taacaaacgc
     2641 tggatttcgg gcaacatata agcaatctgc ggccctaata acacgacatc ggcattctga
     2701 cctttttcac cagccagtgt ttccggaaat gcttcaataa tgaccggaac ttcatatttt
     2761 tctgcctgtg cgcgcatttt tgataccagt aaagagatag acatgcccgc agaacaaaac
     2821 agataaatgt gtttcttttc cataaaactg ccctcatcga cgattatctg tcagccagac
     2881 actccgcaag ccttaacctg cttccatgct ctgggtaact tgcgaaacca acatgacttt
     2941 ttttgtggct gaaaggagta tacggtatag acctgagcaa tagtatttct ttgaccttat
     3001 aaattgtttc tccttgacct gtggttatga ccctcttcac atttcgggca aataattcgc
     3061 gaagataatt aagaactcag gccaaaaaaa accggcgcaa tggccggttt ccgttgttac
     3121 tcaagctttc agacgaattg attacttcgc agcctgtgga tcagtgtcgt attcagcaca
     3181 ggtctgataa ccggagttga tgacatgtcc ggtatcatct aacgcgacaa agtaggtttc
     3241 tgctttacca tcacgttgac ccaggatgta ggtctggcaa gtaccgcgag catggatcat
     3301 gctcacttca gacgaaggtt tacccgcaat ttgtgcaacc tgcgcccggc tcatgccttt
     3361 tttgacgtct ttcaccacag gctgtacaaa ctggtctttg gtacgatcat aagctgtaca
     3421 acccgccagc atggttaata ccgccgctgc actcagaatt cctgccatat tcttgttcat
     3481 attccgtcct cttgtttatc agcgtgttag ataagcctgg aatacattgg gcgctttttc
     3541 aagcccgtga acgaaacggc tccgctttca gaggattcct gtatgacgtt ttaaccacca
     3601 ttcagcccgc tgtcgcttgt cgtttcagta gcaacgggtt agctttaagg aagttttgtc
     3661 ttttctgtct ggaggggttc aatgacattg caacaacaaa taataaaggc gctgggcgca
     3721 aaaccgcaga ttaatgctga agaggaaatt cgtcgtagtg tcgattttct gaaaagctac
     3781 ctgcaaactt atccgttcat taaatcactg gtgctcggga tcagcggcgg tcaggactcc
     3841 acgcttgccg gaaagctgtg ccagatggcg attaatgagc tgcgccagga aactggcaac
     3901 gaatcactgc aatttattgc cgtacgcctg ccctatggtg ttcaggctga cgaacaagat
     3961 tgccaggatg ccattgcctt tattcaaccg gatcgcgtat taaccgttaa tatcaagggc
     4021 gcggtattgg ctagcgagca ggcattgcgg gaagcaggca ttgaactgag cgattttgtc
     4081 cgtggcaatg aaaaagcgcg tgagcggatg aaagcacaat atagcattgc gggtatgacc
     4141 agcggtgtcg tggtgggcac cgatcatgca gcagaagcca ttaccggatt cttcactaaa
     4201 tatggtgacg gcggtacgga tattaatccg ctgtatcgtc tcaacaaacg ccagggtaaa
     4261 cagttactga cggcattagg ttgcccggaa cacctttata agaaagcccc aactgccgat
     4321 cttgaagatg atcgcccttc tctgccagat gaagtggcac tcggcgtgac ctatgacaat
     4381 atcgacgact atctggaagg gaaaaaccta cctgaacagg tcgccagaac aatagagaac
     4441 tggtatctga aaaccgaaca taaacgccgt ccgccaatta acgttttcga tgatttctgg
     4501 aaaaagtaat aatttgcacc tattggattg tgcgaaaaag agtaatttgt tcacgccgga
     4561 tgcggcgtga acgccttatt cgacctacaa aactatgcaa attcaataca ttgcaggagt
     4621 cgaataggcc tgacaggcgt agcacgtcag acggtgtaac ctttgtcatc aacccgcctc
     4681 ttttttaatc gcctcccgcc tgttacactg gatagataac cagcattcgg agtcaacagt
     4741 ggtacggcgt ttaacttctc cgcggctcga atttgaagct gcggcaattt atgaatatcc
     4801 cgaacattta cattcattcc ttaatgactt acccacccga ccaggggtgt atctgtttca
     4861 tggcgaaagt gacaccatgc cgctctatat cggcaaaagc gttaatatcc gcagtcgggt
     4921 gctttctcat ttacgcaccc cggatgaagc cgccatgcta cggcaatccc ggcgaattag
     4981 ctggatctgc accgcaggcg aaatcggcgc tctgctcctt gaagcgcgat taatcaaaga
     5041 acaacagccg ctgtttaata aacggttgcg ccgcaatcgc cagctctgtg ccctgcaatt
     5101 aaatgaaaag cgcgtcgatg tggtgtatgc caaagaggtg gatttttcac gagcccccaa
     5161 cctgtttggc ctgtttgcca ataggcgcgc agctttgcaa gcattgcaga gcatcgctga
     5221 tgaacaaaaa ctttgttatg gcctgttggg actggaaccg ttaagtcgcg gtcgtgcatg
     5281 ttttcgttca gcgctaaaac gttgcgccgg agcatgctgc ggtaaagaga gccatgagga
     5341 acatgcgcta cgcttgcgcc agtctctgga gcgtttgcgg gtggtgtgtt ggccttggca
     5401 aggtgcggtg gcactgaaag aacagcaccc ggaaatgact caatatcata ttattcaaaa
     5461 ctggctgtgg ctgggggcgg ttaattcgct ggaagaagcg acaacgttaa ttcggacacc
     5521 cgccgggttt gatcacgacg gttataaaat tctttgtaag ccgctgcttt ccggtaacta
     5581 tgaaattact gaacttgatc cggcgaatga ccagcgagcc agttgatttc actgaacaac
     5641 agtttacctt gcggttgtaa aagccacagg gtgtgcctgc catcgaacca gcacgcgccc
     5701 tggtcggtag tgagcagttt gtcgcccagt tgccacgccc cgttaatcac aaacaccacc
     5761 ccgccgcgtg agccaaaggt agtaaacgta cgttcggcaa ttctgacttt cgccttacag
     5821 acatccaggc gcgtcataat attgaaatcc atcgacatct gccctgccgt cagtttcgct
     5881 tttaccacct ggtccgccgt aaaggcaaaa ggctgtaacg gttttaaggt atggttaaag
     5941 cggtctgcgc tttcaaggag catctcgccg ccttccagca acgtcactat cctttccatg
     6001 ccgggaaaca aagaaaactc cccattcgcc gcgatggatg caatgctggc acgccagtaa
     6061 aaatcacgtt ttgccggtgg gaaagtgcaa atttcgcgcg tttcaccggc agcgtttcgc
     6121 cacagattca ccgacatttt acgcatatca aagtattcca tactcgctcc cggcccgttc
     6181 ttttcattgt tatcgttcag gtaaccgtgg atggttatcg gcaactcggg tgatttacgt
     6241 tagtggtgat caggcttttt ttacaactgt ttgagaagag aaaagaaaac cgccgatcct
     6301 gtccaccgca ttactgcaag gtagtggaca agaccggcgg tcttaagttt tttggctgaa
     6361 agattattca gcagttgcag gcattttacc ttttgccgct ggacgttctg tcagacgctt
     6421 ctcaaaatta gcattaaatt gctttttctg ttccggcgtc aggatgttgt aaattttgtt
     6481 ctgggtttcc atgtgcgcca gcatgttagc tttgcgctgt tcttccattt ttgcgatctg
     6541 cgcttcagct tttgctttat cgaaggtatc gctggcaatg atgtcatgca ttgcgcggcg
     6601 ttcttccagc ggcggacgtt tcatctggtc acgctggcct ttcatgattt cgtggatctg
     6661 ctgtttctgc gcgtcggtca ggttcaggtc tttgaacatc atgtcctgat gcggaccgaa
     6721 cttgcctttg tggtgcatca tcggcttcgc gtcagccggt gctgcggtag tggtgtctgc
     6781 ggcatgggcc aggttagccg cgccaagagc cagggtagag gcaacaaaca gtgcagttaa
     6841 tttacgcata ttctatatcc ttcctttcag ttatttatta cggctttctt aagtagcgtg
     6901 ccgtgttgac gagattaact ttactgactt tagcgtcaat taatcagagc aacggtaaaa
     6961 caatgaaagt gtaaaaaaca cttttgcgcc aattatggag aaaaaaagaa aatttgatgg
     7021 agagtgatga gagaatatta caacacgatg attttgcaga gattatgaag aactataccg
     7081 gatgactggt gataaataaa gcaaataacc aggattaatc tgtattaatt tataagaaag
     7141 caacttaata cccgcagaat gatttctgcg ggtaagtatt agcttatttt ttcgagcatt
     7201 aatcccgcgc gtaatcccaa cgctaccaac ggattaggga ataacacata ctctacatca
     7261 tgggttacgg taaaacgttc ctctccgtcc tgcgccagca atgttccttt ctcaaacggc
     7321 ataaaattca gcgtgtcact tgccatatgc atttcgaagg acggcgagtg gcgagtaatt
     7381 tgcgaaacca cccgataacg gagcggcggt gttctcacga taccgacact ctcaccagat
     7441 agcagcgcag caattgcgct ggcagttact gcaaactggc gaagatcgtt ttgcccaaag
     7501 ggcaacgctt tgccaagttc caacgtacag gccagcgcgc caaaatgtct gacgctgaaa
     7561 tgggtaaacg taccaccagg ctcctgatgg aacaccagcg cctccagccc cgccgcaccc
     7621 agccacgtca gaaatttctc gtcccaggga atgtcgcgtt gcggtaatac accgaactgc
     7681 ggatgcaagg agccacgaat tgcggtatgt agatcaaggt gccaacgcac agattctttg
     7741 ccctggtcat aaaaatcttc caggcactgt tccagttcgc gcgcccgaca ggtttctccg
     7801 ctttcagcaa atagctgcca acgaccgcca aacattcggt tcatatcgct atggcaataa
     7861 cgtttccctt gcttcagcgc aggaggattc ccgaggatca ccagcaaccg ccaacgtaac
     7921 gggatctcgc tgtgagatat cgcgccaagc aacgcgtcca gcatttccac aggtgccgtc
     7981 tcattaccgt ggatccccgc agaaatcacc agcgcgcctt gcggtggcgt taatggcgtc
     8041 agttccagca caccatcgcc cagccagcgc cagcgaacgc cgttgatttc tcgctcggtg
     8101 ataaccggtt ttttacccgt taaggtcaga gcaagaaaat tatccattgc cccctccctc
     8161 gcgctggaac ggataaaccg aaccgagatt cagta
//
LOCUS       DADMEQ010000147         8158 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.149, whole genome shotgun sequence.
ACCESSION   DADMEQ010000147 DADMEQ010000000
VERSION     DADMEQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.149"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(61..600)
                     /locus_tag="JNZ05_003528"
     CDS             complement(61..600)
                     /locus_tag="JNZ05_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY8566613.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQVWFSLTYQ"
     gene            complement(665..1213)
                     /locus_tag="JNZ05_003529"
     CDS             complement(665..1213)
                     /locus_tag="JNZ05_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8566614.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTINFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(1694..2290)
                     /gene="fimE"
                     /locus_tag="JNZ05_003530"
     CDS             complement(1694..2290)
                     /gene="fimE"
                     /locus_tag="JNZ05_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimE"
                     /protein_id="HAY8566615.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(2768..3370)
                     /gene="fimB"
                     /locus_tag="JNZ05_003531"
                     /pseudo
     CDS             complement(2768..3370)
                     /gene="fimB"
                     /locus_tag="JNZ05_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimB"
     gene            4824..5540
                     /gene="nanC"
                     /locus_tag="JNZ05_003532"
     CDS             4824..5540
                     /gene="nanC"
                     /locus_tag="JNZ05_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HAY8566616.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            5560..6666
                     /locus_tag="JNZ05_003533"
     CDS             5560..6666
                     /locus_tag="JNZ05_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HAY8566617.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            6731..7711
                     /locus_tag="JNZ05_003534"
     CDS             6731..7711
                     /locus_tag="JNZ05_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HAY8566618.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTTLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2427 a         1743 c         1697 g         2291 t
ORIGIN      
        1 tacattgacg tttttattac tcactgtcct gttcctgtta tcacattatc tactgaacaa
       61 ttactgatag gttaaagaga accagacctg ggcattggcg atgccgccag taacccgacg
      121 cccggtagca cgatatttgg cgatgaaatg tagcgaagta gagcctgagt aaagccgttt
      181 ccagtttgct ggaggacgat taatcggtac gaggtttcct tcatcatcaa acaacgctac
      241 accaatattg gaggctatcc ctggcccctc tcccacggag agcacatccg gatttttacc
      301 atccgcgaca ccgtgaaacg ccacacccac acgttcactc accaccgtgc tacattcccg
      361 taaatgaata acaaaaggca ccggtgcgct atcttcccct gccgcatgaa accggttact
      421 gctgatttgc cccatattga ccgtcatttg tttatcaccg gcttcaatcc ggcaagtttc
      481 cgcaataatg acgccctgaa attgcatatt tccgccgggc agcgtggtat tccatttatt
      541 tccggccaga gcaaacattg gcagcaacga cgctaacaga aataatctct tccttttcat
      601 cgttattttt atcgcacaag ggtgggcatc cctgcccgta atgacgtcct tgaacctggg
      661 taggttattg atactgaacc ttgaaggttg catccgcatt agctgcaccc ggggttgcct
      721 cgccgattgc ataataacgc gcctggaacg gaatggtgtt ggtaccgtta ttcagggttg
      781 tttgtgcact aaatgtcgca ccgtccagcg tcagagcatt gcctgttctg tccaggatct
      841 gcacgccaac gtttgttgca ctacctgcag cagaactctg cagagctagt acatcagtac
      901 gcgtagcatc aattgccgta cctaagaagg caacagcggc ttttgtggca acagtggtat
      961 cgcaatcatt cagctgaatg ttaaaaccaa cggcagagct ggttgctcca gcctgcttca
     1021 ggctagcggt acgaacctgt cccaactgaa cggtttgatc aacagagcct gcatcaactg
     1081 cgcaagcggc gttaacaact tcccctttaa agttaatggt cccaccattt accgtcgtag
     1141 tatcggccag agccgctgcg gaactgaggg acagagccga cagaacaacg attgccagag
     1201 ttttaatttt catgctgctt tcctttaaaa aaactatttc taaatcgaca tgggcagtcg
     1261 ttctgtacac tttgttttgt caacgagttt gactgccaac actgcacagt tttcccccaa
     1321 aagatgaaac atttggggcc attttgactc atagaggaaa gcatcgcgca caaacttttt
     1381 cagtttattt gttggcttaa tattctattg ttatctttat ttatagattt ttatattgca
     1441 tgaggtggtt tttggagaga agaatgagga agatgcgtcg agccacagaa acgttagctt
     1501 tacatatagc ggaggtgatg tgaaattaat ttacaagaga aataatttac atatcaaata
     1561 gttagatgct ttttgtcgtt ttttaatatt tttatgcttg agaaaaaata agtaacttat
     1621 ttatgatatg gacagtttgg ccccaattgt cttgtgttta tttgtttttt aactttatta
     1681 tcaataagtt aaatcaaacc tcttctcttt ttaatttttc gtttatgaga ttatttcttt
     1741 cccataatcc ggcaaaacga gcagcattac tggcggtata acgcacagta tggcgaatat
     1801 ttcgatgccc gagataatcc tgaattaaac gggtatctgc accacgctcc gccagttcat
     1861 aaccgcaagc atgccttaac atatgaggat gagtctgcgt tacggttcca gcttcaatac
     1921 cggcatcgcg aataatgcga taggcctgct ggcgagaaag ccgactcccg cggcgagaaa
     1981 taaatatagc gtcggtccgg tcagcgcctt tccagttagc acgttcctgg gtccagcgtt
     2041 ccacggcttc acgctcatca aaacgtaacg ggtgaacggt agaaaatccg ttcttcagtc
     2101 ggcgaatatt tattcgacct tcattaaggt caaggtcctg ataatgcaga tcaagcagtt
     2161 cactaatacg catcccatgc cgatatgcca acagaataag acaataatct ctggctcccg
     2221 ttgccccgta acaaaccgcc tgcatcatgg cctgaacttc tttaccggta agataacgac
     2281 gtttactcac aatagtagta ctcctaactg agatatattt gaatgaatac ctataggaaa
     2341 cctcaatcgg tcaaaattag cccaatagat aaaaagataa ccacacacga agttatgtgg
     2401 ctatcagtca attacattaa acatcataca aaaataaaat atcactgatg tgacaacatc
     2461 tattttcatt gatttgattc aatataacaa tagattacag aggattaact tccagttact
     2521 taaaaaataa agaaaaaagt gtggaaacag gagaatttaa gtgatgtgta aacttatgaa
     2581 tgattttgtt tatttatttt gaagaacata aaccattaga ttttgtatgg gtatggttaa
     2641 cttaatgttt attatgataa ttaagaatca cacttacaat aacatcaatt aataaatggt
     2701 taatcatttt tgatatatcg taagaataat gtagttttta acaccatccc tggtatctca
     2761 actatctcta taaaacagca tgacgctgtc gtcctctggc tctatcccag atgccgtaaa
     2821 aacgccctgc attgctggcg gtataccaga cagtatgacg aatattgcga tgcccaagat
     2881 aatcctagat aagtcgcgta tctattccta tattcgccaa agcaaaacca cacgaatagc
     2941 gtaacatgtg cggatgaatc tccagtgaca acccggcatt accaccagaa gtcgagataa
     3001 tatggtaaaa ctgttgccga gaaagcggat tccccttacg tgataaaaat acccactcgc
     3061 tctcagcatg cggataagaa gtacggatac tcaaccagtt ttttaaagcc tgaacttctt
     3121 tgttcaatag cgggtgcgtt gttgaaaagc ctttttttaa tcgatgtata tatatacact
     3181 ttgctttaag atcaatatcc gaaatcctca atcgacaaat ttcactcgcc cggaaaccat
     3241 gaataaaaca aagcaaaatc agacaataat tacgtgttgc atgaggcccg gtatttgctg
     3301 ctttaaggag tgactcgatt tcactatggg taaggaagtt cctttttttg ttatcagcct
     3361 tattcctcat cgttttccct tataattaca gacgcgcact agctgtgctg ggttagcaat
     3421 aatccaacat ttttatcctg attatgttta taaaagcgaa cgtttgctta ataacttacg
     3481 taagtggacc agttcttctg agcgaactta aatggagtag cgatagttac ttataataac
     3541 tttgccatac aaacgccatg ctcttgcatg ctatgtacct ttatatattt atcaatctga
     3601 tacgaatcta ataataaccc tcatcattaa tgcaactaat cttatctata tatcatgtga
     3661 tatgttctgt aacaagtaaa atcaacataa aaaattcagc aaaatttaat atttgttcaa
     3721 ctagccgtct caaatatgac attattagtt aactgcattt gccacaaaat ggctatttca
     3781 tggaacgcag taatttctta gtggctatta tcatgctaat tcttagatcc ctagcgatta
     3841 ttcctgcctg gttcgtcagc ttaggccatg agattgttac attccttttt atcctggaga
     3901 tatgggggaa cgcaggcgac gctttttcat ctacatccgt tggtaaatat agctttggtc
     3961 actacgactt aagccttcct caatcacagc tattcgccgg gcgatacggc aaacaaacta
     4021 agtgaaacat catcctgaaa acaccaacat caacaagcct ctccagatcg acatcaggag
     4081 tgaccagtta caagccacaa accaagaaac atatttcatg atgagaatta tgctcagcct
     4141 gatggcgagt aaacccttat tttcaccact ctcactgagt tagcttgcaa atctttcttt
     4201 gtttagattc taaataaatt atttataaaa catatagtta aaagaaaatc tttagttttt
     4261 gaagaccacc gcaagtgttc gtctggcttc acatgacatc ttcttcttag aaaaagatcg
     4321 acatattttg tgacgcgaat tgcaaatctg gttttgttgt atggattgcg tgatttttga
     4381 tctggtataa caggtataaa gatgcaccac gatagtcaat gagactgggc atctcgtaat
     4441 ctatggcaaa catcacttca gttctttcac atcgggtgat gaaaatgcac ttcagtctga
     4501 aaggaatatg aaaatgagat caacagacac tctattttat gactctgggt aaaatgggtt
     4561 gagtaagtga tataacttac gaatattcaa atcaattaaa aatcagaaga gattttatac
     4621 tcaggtattt aatctggatc tctgtttatt taaataatgt taaaagagaa ttttcacagg
     4681 agaccttata caaaaaatat aaaatacagt tacccgttgc caaagacact ataagcctgg
     4741 caaaaaaata ttacacaaca taaatgctaa ttgtttatgc gggctttgta ttgctttctg
     4801 tatcctacaa atgagtgaaa tttatgaaaa aggctaaaat actttctggc gtattattac
     4861 tgtgcttttc gtccccatta atttctcagg ctgcgacact ggacgtacgt ggtggatatc
     4921 gtagtggaag ccacgcctat gagactcgac tcaaagtcag tgagggatgg caaaatggat
     4981 ggtgggcaag catggaaagt aatacctgga ataccattca tgataataaa aaggaaaatg
     5041 ccgcactcaa tgatgttcag gttgaagtta attacgcaat taaacttgat gatcaatgga
     5101 cggtgcgccc gggaatgtta acgcatttta gcagcaacgg cacacgctac ggaccctacg
     5161 taaaactgtc ctgggacgcg acaaaagatc ttaattttgg cattcgctat cgttacgact
     5221 ggaaagctta ccgacaacaa gacttatccg gtgatatgtc tcgtgataac gttcatcgtt
     5281 gggatggata tgtcacttac catattaata gtgatttcac cttcgcatgg caaacgacgc
     5341 tatacagcaa acagaatgat tatcgctatg caaaccacaa gaaatgggcg acggaaaatg
     5401 cctttgttct acaataccat atgacgcccg atattacgcc atacatagaa tatgactacc
     5461 ttgaccgcca gggtgtttac aacggcagag ataatttatc ggaaaacagt tatcgcattg
     5521 gtgtgtcatt taaactgtag tagacaggag acagtcacaa tgaataaaac tataatggcg
     5581 cttgctatca tgatggcttc atttgccgca aacgcgtctg tattaccgga aactcctgtg
     5641 ccatttaaaa gtggtaccgg agcaattgat aacgacactg tctacattgg tttaggtagc
     5701 gcaggtacag catggtacaa gctagataca caggccaaag ataaaaaatg gactgcgtta
     5761 gctgcattcc ctggtggacc gagagagcaa gcaacctctg catttattga tggcaatctg
     5821 tatgtgtttg gcggcattgg caaaaacagc gagggattga ctcaggtatt taatgacgta
     5881 cacaaataca accccaaaac caatagctgg gttaaattga tgtcgcacgc gccgatgggc
     5941 atggcgggtc atgtgacttt tgtacacaac ggcaaggctt atgttactgg cggtgttaac
     6001 cagaatatct tcaatggcta ttttgaagat ctcaacgagg ctggaaaaga ttcaaccgct
     6061 atagataaaa tcaacgccca ctattttgac aaaaaagcag aagattattt cttcaataag
     6121 tttctgttgt cttttgatcc ctcaacacag caatggagtt acgctggcga atcgccctgg
     6181 tacggaacgg ctggtgcggc ggttgtgaat aaaggtgata aaacctggct tattaatggc
     6241 gaagccaaac caggattgcg aacggatgcc gtatttgaac ttgatttcac cggtaataat
     6301 ttaaaatgga ataagcttga tcccgtctca tcaccagatg gcgtcgctgg cggttttgcg
     6361 gggataagca atgattctct tatatttgcc ggaggggccg gattcaaagg ttcacgagaa
     6421 aattaccaga acggtaagaa ctatgcgcat gaaggcctga aaaaatcata tagcactgat
     6481 attcatcttt ggcataacgg gaaatgggat aaatcgggtg aattatcgca aggtcgggcc
     6541 tacggagtat cattgccctg gaataatagt ttattaatta ttggcggtga aactgcaggc
     6601 ggcaaagcgg tgacggattc agttttaatt tctgtgaagg ataataaagt cacagtacaa
     6661 aactaacgct tcagggcccc ggtaaagggg ccctgctatc aatttgtcat ttgtattacg
     6721 cgaaaattat atgaacgcaa taatatcgcc cgattattac tatgttctta ccgttgctgg
     6781 tcagtctaat gccatggcgt atggcgaagg actgccatta ccggacaggg aagatgcgcc
     6841 tcattccaga attaaacaat tagcgagatt tgcgcatacg catcccggag gcccgccatg
     6901 tcactttaac gacattattc cactgactca ctgcccacac gatgttcagg atatgcaggg
     6961 ttatcaccat cctctggcaa cgaatcatca aacacagtac ggcaccgttg gccaggcact
     7021 gcatattgca cggaaattac tgcccttcat tcctgataat gcagggattc ttatcgttcc
     7081 gtgttgccgt ggcggatcgg cttttaccgc gggcagcgaa gggacatatt cagaacggca
     7141 cggagccagc catgatgctt gtcgttgggg aacggatact acgctgtacc aggatttagt
     7201 cagcagaacg cgagccgcac tggcaaaaaa tccgcagaac aaattcctcg gcgtatgctg
     7261 gatgcaaggc gaatttgact taatgaccag tgactacgcg tcacaccctc aacactttaa
     7321 tcatatggtt gaagcctttc gtagggatct aaaacaatac cattctcagc ttaataatat
     7381 tactgacgcg ccgtggtttt gcggcgatac cacctggtac tggaaagaaa atttcccgca
     7441 ttcgtatgaa gctatttatg gcaattatca aaataatgtt ttagccaata ttattttcgt
     7501 cgacttccag caacaaggtg aaagaggact gacgaacgcg cctgatgaag acccggacga
     7561 tttaagcacg ggatattacg gttcagcgta ccggtcaccg gagaactgga cgacggcact
     7621 gcgaagcagt catttcagca cggcagcccg tcgggggatt atttctgaca gatttgtaga
     7681 agcaattttg cagttttggc gcgaaaggtg agcggggagt ttattcatgg gggatgtcct
     7741 tgttcgttct cagagtagac aacaaaaaac aaatgagatc aaggtaatat ctcttcactt
     7801 gcgggctcga acagcaggca attgccaata taataatctg gcacaagggt gaaaaacgcg
     7861 caccgcataa accgttgtta ttgcttacag attagccgga tgccttaata gacatccgcg
     7921 ccttttcgat tatggctcgg aaatctacga acatctacac agtttactgg aacgttttgg
     7981 gccacagcgt tcgcagcacc gtcctgatat gccgttctgg cgattataag tcgacggatt
     8041 ctggcaaata gacgatgatg atatgcttaa tgatgttcaa agaaattagg ccattattat
     8101 attattgctt gcattttctt tcattcctaa aagcacccgc cattaatccc aggcgcat
//
LOCUS       DADMEQ010000148         8091 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.150, whole genome shotgun sequence.
ACCESSION   DADMEQ010000148 DADMEQ010000000
VERSION     DADMEQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.150"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..892)
                     /locus_tag="JNZ05_003535"
     CDS             complement(<1..892)
                     /locus_tag="JNZ05_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAY8566619.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPS
                     SVNVLSELPGRTVPYEAAEIRPQVGGIIIKRNFIEGDKVNQSDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGS"
     gene            952..1230
                     /locus_tag="JNZ05_003536"
     CDS             952..1230
                     /locus_tag="JNZ05_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566620.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1231..1758)
                     /gene="gadE"
                     /locus_tag="JNZ05_003537"
     CDS             complement(1231..1758)
                     /gene="gadE"
                     /locus_tag="JNZ05_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HAY8566621.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2557..3129)
                     /gene="hdeD"
                     /locus_tag="JNZ05_003538"
     CDS             complement(2557..3129)
                     /gene="hdeD"
                     /locus_tag="JNZ05_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HAY8566622.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3384..3716
                     /gene="hdeA"
                     /locus_tag="JNZ05_003539"
     CDS             3384..3716
                     /gene="hdeA"
                     /locus_tag="JNZ05_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HAY8566623.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3832..4158
                     /gene="hdeB"
                     /locus_tag="JNZ05_003540"
     CDS             3832..4158
                     /gene="hdeB"
                     /locus_tag="JNZ05_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HAY8566624.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4222..4868
                     /locus_tag="JNZ05_003541"
                     /pseudo
     CDS             4222..4868
                     /locus_tag="JNZ05_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4910..5440)
                     /locus_tag="JNZ05_003542"
     CDS             complement(4910..5440)
                     /locus_tag="JNZ05_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8566625.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5596..6162)
                     /gene="slp"
                     /locus_tag="JNZ05_003543"
     CDS             complement(5596..6162)
                     /gene="slp"
                     /locus_tag="JNZ05_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HAY8566626.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGLGAPYYTNAVSQVTPELVK"
     gene            complement(6409..7632)
                     /locus_tag="JNZ05_003544"
     CDS             complement(6409..7632)
                     /locus_tag="JNZ05_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566627.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGKDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
BASE COUNT         2350 a         1569 c         1697 g         2475 t
ORIGIN      
        1 ggctaccttc atccactaat gccgtgacat acatgccagg cagcaagtca ccatttgggt
       61 tggggaaaat cgcccgtaac gtcacggagc ccgtggtttc atcaaccgtc gggtcggaga
      121 atttcagcgt gccggtctgg ctgtaacgtt taccattttc cagattgagc tgtactggcg
      181 tactgccctg aacctgtttg atttgcccac tggcgacctc ttctttcatg cgtaagaaat
      241 cttgcaccga ctgcgtgaga tcgacataaa tcgggtccag acgttgtacg gtaaccagcg
      301 aatctgcctg attagcggta acgagtgcgc cgacggtcac cgacgatttc ccgctgacgc
      361 ccgtaatcgg cgaggtgaca ttcgcgtatt gcagattgat cgtcgcctgt tcaacagccg
      421 ctttggcgac ggtgacattg gcttctgctt cattcagctg ggtgcgcgcg gtgtcgtaat
      481 cctgacggct aacgtagttg gtcttcagca acgatgcctg gcggttaaag gtgatgcggg
      541 cattgctggc ggtagagagc gctttcgcca gcgagccttt ggcggagttt agctcggcct
      601 gtaaaggtgc aggatcaatc tgatacagcg aatcgctctg gttcacttta tcgccttcga
      661 taaagttgcg tttaatgata ataccgccca cctggggacg tatctcggca gcttcataag
      721 gaacggttct accgggcaat tcgctcaaca cattgaccga actaggggag agtgtgacga
      781 caccgacctc aggcgtcatg gcggcggcgt tttccgccga tttgtcatcg caggcggtga
      841 gcatcgcgcc gcagaataac aacggtatta acagctttct tcttctgttc attttagtcc
      901 ctgaaaattc ttgagaaata acggttcttt aaattgcgcg cgaggagaga tatgagggat
      961 ttacagacca gcggcatagt ggggttgtca gccagtaagg tgggatacag gcacagtgat
     1021 cgacatggtg aggtcaacga caaaaaaaat gttgccgttc tgccaacagt tcctgccagc
     1081 attcgggcaa cgaagggttc tactggtgga tacacatacc aggggaatca agtcgtttta
     1141 gcctctcctt tagtaatcac cgggggtaat gaaatcagca tctgcatccc tcgtcatgcc
     1201 agccatcaat ttcagttgct tatgtcctga ctaaaaataa gatgtgatac ccagggtgac
     1261 gatgtcgctc atacgtctta gtttcagctt gtacataatg ttgaatttct gaatctttaa
     1321 tgtttgttgc gaaatattga gcgtcgacgt gatattgctt tcatttttat ttttcagaat
     1381 cagttgcata attttgaact gattatgtga aaaacataat tgtgaattct tatggggcaa
     1441 gtgtttacca taagtaatct caagaagatt tttgatactt tctttgcggc taacaaaata
     1501 aataggcgct ttagctttta gtttactgat gtgataaggt gccaaaacaa taattctctc
     1561 ggcatctaat ttctccagaa atttaatcgc ttcttcatca aggatatgat tgtgataggt
     1621 atcaatgata accttgaagg gtttattgcc tacttttttg atctctgaca gggaattgat
     1681 ttttatcatt tcgtgattat ctttcaactg ccaaaagccc tgtaaaagaa aagaatcttt
     1741 cgtcatgaga aaaatcataa cttgctcctt agccgttatt gtttcgaaca tgtcatcctt
     1801 ttcattattt acatccttgt ccgaatcgtt gttcaatata gtaaacgcct ataagaactt
     1861 tgcccttcgt gaactaatac gtctattaac actaatcaat acttcagatt tatccatcac
     1921 gcactccata tattgatcct aagtaagatt cctggttgtt atcagcttgt aaatagtaaa
     1981 caaaaagtta acgtttgcgg attactgccc aagaataagt tatcaataac cagtttgtga
     2041 tctctgaaga atattactaa agttaaaatg taatccgatt taaatatcga gtctccttgt
     2101 ttcgacttaa gctggcaatt ggattgccag ctttctttta acgcattgaa attacctgag
     2161 taaataacgc atttatgtct tcaggttttt atatagcgtc tggagacacg gatatttatg
     2221 caatgtaatc aacgccagtt gcaaaaaaat gaacccggat tacttattta accgcccata
     2281 gcatcaacgc ttacggaact ctttgttcac tttcccacag cacttttaaa acattaaacc
     2341 tgactacgga aaatatcagc catgataatg tttgaaatgg ctaatttgcc atggagtgaa
     2401 aaaaattaga tgaaattcag taggttgaaa taatcactag caggtaatta tttcaatgat
     2461 agtgcgcaat tgatctacaa cactgcgtag cggagagagt attaatcgga tcatagtcac
     2521 atcaagtgac tatgatccgg gtgacaaccg gggtaattat tgctgcttaa cgaacaaact
     2581 ggcgaagctg aacaggctgg cggcgctaaa tatcagttca attcccacca gtgtggaaac
     2641 cagcgttaca gacaccatcg gcgttgcacc aaggaatatc caggcaatga cgatatccag
     2701 cacaccaata acgagctgta gccagctgcc tttcattgaa cgctgacgat accaactcat
     2761 caggcgaata acccctgcaa cacagaacaa accggcaata aatgccgcaa tggcaaaaat
     2821 gcccagctcc ggtgcacgga tgaagaaata gccgatcaat aaataggcga ctgcgacgag
     2881 gaaaccggat aataccggcc agaaattatg actgcggttg ctgaataacc cgacaataag
     2941 cgcaataccc gagcagatta ataatgcacc cactactgtg cttaaaatat cgccagagac
     3001 gaacgggaaa ctgatacaca gcaacccgac gataaacagc agcacggcaa taaactggat
     3061 tgctctgcga tgctttttca gcatctccag atcaaacttc aaaattgttg ccttatctat
     3121 atataacata gaaccaccct ataaaattaa gaagaaaatc ccctgctatc aatctatgcc
     3181 aaaaacgcgt ctaagaatgc agtcgattta ataaaaattt cctaattgca gtatctgatg
     3241 catctgtaac tcattgtatt gaaataaaaa tatctgattt tgatattttc catcaacatg
     3301 acatatacag aaaaccaggt tataacctca gtgtcgaaat tgattcgtga cggctctttc
     3361 actttatagt tgaggatatt acgatgaaaa aagtattagg cgttattctt ggtggtctgc
     3421 ttcttctgcc agttgtgagc aatgcagcgg atgcgcaaaa agcagctgat aacaaaaaac
     3481 cggtcaactc atggacctgt gaagatttcc tggctgtgga cgaatccttc cagccaactg
     3541 cagttggttt tgctgaagcg ctgaacaaca aagataaacc agaagatgcg gttttagatg
     3601 ttcagggtat tgcaaccgta accccagcta tcgttcaggc ttgtactcag gataaacaag
     3661 ccaactttaa agataaagtt aaaggcgaat gggacaaaat taagaaagat atgtaattcc
     3721 gggaatgcgt tacatcgtac ttccttgcat attgaacagg ccggaatatc ttctttaaaa
     3781 gcagctattc ctcctgttca tatataatct ctatattgaa tgggttacaa aatgaatatt
     3841 tcatctctcc gtaaagcgtt tatttttatg ggcgctgtag cggctttgtc actggtgaac
     3901 gcacaatctg cgctggcagc caatgaatcc gctaaagata tgacctgcca ggaatttatt
     3961 gatctgaatc caaaagcaat gaccccggtt gcatggtgga tgctgcatga agaaacagta
     4021 tataaaggtg gcgataccgt tactttaaat gaaaccgatc tcactcaaat tcctaaagtg
     4081 atcgaatact gtaagaaaaa cccgcagaaa aatttgtata ccttcaaaaa tcaagcatct
     4141 aatgacttgc cgaattaatg aggtacaagt aaaaaggagt agcaagttga gccatcttgc
     4201 tgctcctttt tgcattttta tatgacagca gaatttatta tacgtcttat actcgcggca
     4261 attgcctgtg gcgctattgg catggaaagg caaatgcgcg gcaaaggagc agggttacgc
     4321 acacatgtat taattggcat gggaagcgcc ctgtttatga ttgtttcgaa atatggtttt
     4381 gctgacgtgc tgtctttaga tcacgtcgga ctcgacccca gccgtattgc tgctcaggtg
     4441 gtgacgggcg tcgggtttat cggtgcaggt aacattctcg ttcgtaacca aaatattgta
     4501 ggtctgacga cggcagcgga tatctgggtg accgccgcat aggtatggtt attggcagcg
     4561 gtatgtacga actgggtatt tatggttcag tgatgacctt gttggtgctg gaagtcttcc
     4621 atcaattaac cttccgcctg atgaacaaaa attaccattt acagctgaca ttagtgaacg
     4681 gtaatacggt atcaatgctc gactggttta agcagcaaaa aataaaaact gatttagttt
     4741 cgttgcagga aaatgaagat catgaagtgg tcgccattga tatccagctt catgccacaa
     4801 cgttggtaga agacctgttg cgattattaa aaggtatggc aggggtgaaa ggcgtttcaa
     4861 ttagctaatc tgtgtggcag cgtagccaga ctcaccgtaa gcctgaaatt cacaccagat
     4921 aatcaatatg ctgatggcgt accagctcat tgatccgctt aagattgagg atcatcatga
     4981 tattgtgttt atggcagtag aacgttttta gcgatatttt cagtttctcc agaatatctt
     5041 cagttgtcat ttgattcgca atgcagctca gaacctgatg ttgcttgcgc gaaagtgaca
     5101 cattcgcttc aggcacagag cagtaactgc cattgatgag tgcgacaaaa tcaatgatag
     5161 tgatagtccg cggaacaaag gtcaccggta ctttccccag acagcgttta atattaaagg
     5221 gggagaaaac aatgacatgg acaggtttta atcgttcaat ttgagtgaga aaattagaat
     5281 gaaatacatt attcattaat gtatcaataa tgacgaaggc attctgatgc aacattacgt
     5341 cgatctcttc ttcgttctgt atatgaacga cattaccttt actcagaatg tttttcatcg
     5401 cggtgaagaa catcgtatcc ctggtaatta taagaaacat aaccgactcc tggtgcggac
     5461 attaacttta taatcagttt gccaaaggta attaacagga cagggcttat aataataata
     5521 gttagcttac tgtatattaa ttttatgtaa tacatgatgc agatgctgag aaagcatctg
     5581 catctttcgg tggtgttatt tgaccagctc aggtgttacc tgactcaccg cattggtgta
     5641 gtaaggcgca cccaagcccg gttccggcca gaatgcacca tagccgtaat cccacaggtt
     5701 ataggtggta ttaaccactt ctctcaaatg ccacacctgg atgccctgca tattcacttc
     5761 caggaagtta tacgggactt tattgataaa gcctggttgt tcaccctgaa tggtaccgag
     5821 gatggtaaca aagtgattac gatagttcac tggatcaagg aagccgcttt gtctggcgag
     5881 cagtcggccc tgatagttgg cttcaatatc aggcttcgca tagctatcca acggtaatac
     5941 agcgatttct aacaacgtat ccgttttgcc attgataacg ttgataacct tcccaccaaa
     6001 gcgcgcttgt tgaccaacat ataaccccgg ctggttatga acagcaacaa aacttttttg
     6061 aatatcaggt tggttattgc ctttgatatt ttgcggaatt gaactacatg cggcaagcaa
     6121 aaatgaaagg ctgaggatga gtgcaccttt tgtcatgttc atgttactat ccttatcaac
     6181 aaatattaaa accataatag atatttcttt atagttttca tctgattctg aggtgatatg
     6241 cctgatagta acgtaaacct gtctgatgtt tatatccctt ctgtttagtt caattccgtt
     6301 attttggggc gtggtatgag tatccatcgc ccaataatat aaccctttgt tttaaaatta
     6361 cataaaatct atttttattc aataattata aagtgctaat aatcaatatt atggtattat
     6421 attctgataa tgatttgata agcgaattct tttgccagat ggtatttcta tttccacccc
     6481 ataatttata tctttttcat taggcagatc tctctttaaa taaaaggggt atgaaatctt
     6541 ttcgggttga agtgtctcgt aatgaaatgc tatgaattca tttttagaca ttgttgccca
     6601 tttttgaatt ttattgtata gagagggtgt tgtaaactct tgaagttgtt tataacatat
     6661 ttttttgaac tcttgcttat attttaatat gtcatcaaat gaaagattca ctacctttgc
     6721 ctctaagaaa tcaattattc tcgaagcaat ttccgctttc gcgcgtaaag agttgtcatt
     6781 aatagttaac aatgtagaaa acaagccatt gatttgtttg tcaagatgtt gttcatattt
     6841 atgtagtttg gggatgggat tttccttgtg tgaaaaaata gtgttaaata aatttgcgtc
     6901 agtgaaattt atattaagta gatcttcaaa ataaaatttt gataaggtta ggactagctt
     6961 ggacatatct ttccctgtca tgttgaaatc aataaagtcg catccagcaa gggcttccat
     7021 cctattttca tttttactgt gctcaatata cagcttcgcc atttttaagt acaagctttg
     7081 aatgtctctt atatcggttg gtaagtcagc tcgttgccca gcaaaatttt tattatcgga
     7141 agaaacggtt tggacactga atctttcggg tattccttct tctacgagaa ttttagttat
     7201 gattggattg ttgggttctg gattaacgac aacaatgatt tgacatacag aatccttatc
     7261 cttttcgtat ctaatgttat gaatctgatt aagtattgat tgtgtctgtt gcgtaagtgg
     7321 tgtcgaactt atcgtttcaa tgttattaat aagacttaca agttgaacca caacttttga
     7381 gtagtctgca tttgtatatg tggtgttcgt gtggcatgca ttaatttgtt ggagttgttg
     7441 ttgcgctaaa tcaaattgtt cagggttgta tccttctcca gagaaggtgg aattaatggg
     7501 ttgttgttct tcccaattat cacaaggcca cttcggtgtg tctggcgttt taacattatc
     7561 ggtatttgtt gtgcagttat aaatatcgcc gtgatatgta tttattatac ctggggtaaa
     7621 gtcaattgac ataacattcc ttattcatca tctgttttat gctaatgttt ttaaattgga
     7681 taattagaac aggaatggat tttcttctac acacaataaa tataatgcgt tatatcttcc
     7741 tgatataatt acctttaaat tccctttcat ttccacccgg taatatttgt gtataaaaaa
     7801 agggcagtga tagtatcgta aaggaaaacg ttaacaacta tcaaaaagcg ctcattttaa
     7861 aaagttatta tgttctccga tagtgtgtga agtaatattt ttgtgatgtt ttagatgtgt
     7921 aataatgaac caggttaaag tgtatttaaa aatgtgttgt attagcgact gaaatctgcc
     7981 ttttaaaaag gaggagggta ctgagacaaa gagtcattgc ttctccgata aaatgtcaga
     8041 gaagcatgat tacgtggtta ttgtctgttg atgacttgtt gaccgagccg t
//
LOCUS       DADMEQ010000149         7933 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.151, whole genome shotgun sequence.
ACCESSION   DADMEQ010000149 DADMEQ010000000
VERSION     DADMEQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.151"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            155..482
                     /locus_tag="JNZ05_003545"
                     /pseudo
     CDS             155..482
                     /locus_tag="JNZ05_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(512..3424)
                     /locus_tag="JNZ05_003546"
     CDS             complement(512..3424)
                     /locus_tag="JNZ05_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566628.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGSIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3427..3543
                     /locus_tag="JNZ05_003547"
     CDS             3427..3543
                     /locus_tag="JNZ05_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY8566629.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3907..4749
                     /locus_tag="JNZ05_003548"
     CDS             3907..4749
                     /locus_tag="JNZ05_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY8566630.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSLGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGRECLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4752..5840
                     /gene="capU"
                     /locus_tag="JNZ05_003549"
     CDS             4752..5840
                     /gene="capU"
                     /locus_tag="JNZ05_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HAY8566631.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5845..6795
                     /gene="virK"
                     /locus_tag="JNZ05_003550"
     CDS             5845..6795
                     /gene="virK"
                     /locus_tag="JNZ05_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY8566632.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNQIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6860..7804
                     /gene="lpxM"
                     /locus_tag="JNZ05_003551"
                     /gene_synonym="msbB"
     CDS             6860..7804
                     /gene="lpxM"
                     /locus_tag="JNZ05_003551"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY8566633.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2134 a         1775 c         1886 g         2138 t
ORIGIN      
        1 gtttgggcat aacggtgctt tcagtaagta cgacaggtac tgtggaggca cttccgtaaa
       61 gaagggcatc aagctcctgc tccgacatac ctgcaggcaa aggccatgaa agccctgatt
      121 tccggaagcg agcgaacagt ccacaaactg ttgaacgtgg tgtcagcggc ggacagggat
      181 aagccctcgt cagcagttcc gtcagcactg cgacagcgtt ctttacccgg tcaaaacagc
      241 gttgcggtgc accacgcctg acggatgaac tgcgtgctca gggttaccac tttaacgtaa
      301 aaaccgtggc ggcaagcctg cgccgtcagg ggctgagggc aaaggcctcc cggaagttca
      361 gcccggtcag ttaccgcgca catggcctgc ccgtgtcaga aaatctgctg gagcaggatt
      421 tttacgccag tggtccgaac cagaagtggg cgggagacat cacgtactta cgtacagatg
      481 aagttcggtt acatcctgtc agcacggaac ctcacgcttt ttgagcggca cgacggatcc
      541 cccccaccca gtatccgaca gacttttcac tgcgttcgta aaaacccttc ttccctccca
      601 gcatatccac taattccggt gacatatcct gcagataagt ctgcaaatca tgccccagat
      661 cttccggtga ctgcaactca taaaccgcgc gccacacatc accctgcctt attttaagcc
      721 ccgggttaag aatatactgt ccgtggttca gcctcagaaa aagctttttt ccataaggat
      781 ttttgctgtt aacttcatgt tgagagagct ggctgctgat ataactcctt cgtttgcggc
      841 gttccggcag caatgcttca ggaagatgaa ccacacactc tgccagtcgt gccgcactga
      901 aggcttccga aaaaaacaga gcacttccca gatgcgtgtg gaacagcgcc tgcatcagga
      961 tcaccagaaa cattgccatc tgccggttgt ccagtttgat gaggcgtcct tccacctgca
     1021 gtgacagact ttcaggtttc agcagggtat acaactgcgc acttttctgt tgcgcataac
     1081 ggggcgtact gaccgccgct gacagaactt gatgataagc cgccagcccg tcattaccgg
     1141 tcagcaaggg atctgcacca cgctccagca gtaactgaac cagtgcaata ttacctgccc
     1201 gggctgctga catcagcgga gtaagattaa acggcgaacg gtgatcaaca ccataacgtt
     1261 caatatcccg caacacggca accgggttat tcattgagta tggccggaaa tacttcagct
     1321 ccagtaactg cagagtcttc gttctgggct gccttgtggg cttaaaatct tcccggtaca
     1381 gtgcattata cagcgggaac agggaataaa tcagactgta ctccagcgcc tgcagttgta
     1441 tggttttatc ggctgtacct gcgaggatat gctgtttcca ctgtcgtgca tcctctgcgg
     1501 tgataatctg ccatggcatc tcagatgtct gcaggatccg gctacggatg gtgtccgcct
     1561 gctcctgctt cccctgtttt tcaagcagat gtgcggtttt ctgccactca tcacggctgg
     1621 aaatctcttc ctgcagattc agaggctctt cctgatgcgt gagcgcaagc agactccaca
     1681 gaggatgctc cacctgctgt tcaacaagat acacattgtg tgtagcacgg gtgagcgcaa
     1741 catacagcgc gttggtaaaa aatttataga tttccgctga acgatcctgc ctgtcccgcg
     1801 gacgggaata acgggcctcg ccctcaagat cagccggtgt aagaccctcg caaatatcat
     1861 caaatgcctg ccgggcagcg cttactatat tgtaaaggat caccgtttcg tattccagcc
     1921 ccttaacctc ctgaaccgaa aagaccagcg gcgtactgaa ccagcatcgc gcctgtgctt
     1981 tctgttccgg atgcatcaca atgaccgcta cacgattcga aagtgaggtt ttggcattca
     2041 gttcctgctt tgtctcttcc ctgtcgtcca gcaaccggat gctcccttct gcctgaccgc
     2101 agcttctgac aaaatggtga ctttcccgat caatagcact aaagcggacc tgtttgagtc
     2161 tcagcaaccg gttagccagt gcagtgacgt ggtgcccgtt acggtaattc gcctgcagga
     2221 tattaacggt tgtgtcgttt ccctgttgct gccggaaaaa gagacttttg agcgaactcc
     2281 atgagaaaaa gctcggatga actatctgat tggcatcacc acagagtaaa aactgaccgg
     2341 gatgacgtag cgttttcagt accagttgca gttgaacagg cgtcatatcc tgaacttcat
     2401 caacaaaaat gacatcataa cggggagttg ccaacgaaag atactgatga ctgagcagat
     2461 tactgtcata ctgatgcgac tgtttcagcc atgcgatata acgttcaaac aggacataga
     2521 ccgtcgggcg gtcctccatg ccatagagcg actgccgtat ccccagtgac agatacgcct
     2581 cccgggacag cggcccattc ccggaggcaa ccgcaccgat cacgcccctg aactcctcat
     2641 acagggtatg ggcagccccc agtgcggcga tagccctgtt gcggggcagc cagtcgctga
     2701 acgcactgaa agtcacctcc ctgccttccg gaatgcgcag agtttccaga aattccgtca
     2761 gcgaaagaaa gtcaatattc tgtacctcac tcccttcccc tgaagcgtca tagagagtac
     2821 gcgctttttc caccagaaat gaggaaaggc tgagatacag tatatcacca gcagcctgct
     2881 tcattttttc cagtaccagc gccgtcttac cacttcccgc cgagccaaca atcaccagcg
     2941 gtagcgggtg ttcgtacagt gcctgctgat catcatcaaa ggacagcatt ttatccaggc
     3001 ggtggaaacg cccatgagac ggattgatat acgtcagcgc ttcagtcgcg atatcaacag
     3061 gatccggcac tggttgttgt tgtaagcgcc cttcatcaat caccacgttt ctgcgaagaa
     3121 aacgtgaagt gttataggca tgattgcgaa tatattccag aacaaggcag atagtttcac
     3181 cgtggtagcg atacagcgaa aacagtaatc ggtcccggat actgagccgg gcgcggtaaa
     3241 gattggtgtc aattttacga acatcagcct gaacaaagtt atcggtggca atagcctgtg
     3301 caaatttttt ataacccggg atcgtgtcag gattcagagt atgataaaga cagattttca
     3361 tggctgccgg tttccgtcgt gcaaaaaaca tgctgatttt acgaaggaat gccttactga
     3421 acaaccttgc ccggaatacc ccccgaaatc agatggaaca atcagaaaaa tgctctgcag
     3481 ttatggaata aagaaggtgg ggtggtacgc cgggagggcg atccgctgga aatggcgcag
     3541 tagctgcagg cctgtcatga tgccggaata gaagttcgtg tacaggttaa tgaaagcgtc
     3601 accccgtaaa taaactctgc gctgaaattc ctttttttca ttggagcacc tgtgttgttc
     3661 tgaggtgaac atatcacctc tgttcaggta gccaaattca gtgtgccacc tcaaggttct
     3721 tagtgagtcg tttttaaaag atatgataat atatcattgt ttatgaattg ttgatgttta
     3781 atttagtaaa ttatatgaaa tatgccatgg gtttgctatg ttattcatcc agtatctgga
     3841 aataaaatgc accagcttta ttgggttttc cttttgtctc tgcttactag gtgccagaaa
     3901 ctttaaatgt taaacgaagg gggcattttg ttcaaagcca atcatgttcc ggtattaatg
     3961 tatcatcatg tcagtcattg ccccggactg gttacacttt ctcccgtgac attccggaag
     4021 caaataaaat ggttggctga aaacaactgg aaaacactgt catcagatga actggagttt
     4081 ttttatcggg gcggaaaact cccccggaaa agcgttatgt tgacatttga tgatggatac
     4141 cttgacaact ggtttcaggt atatccttta ttaaaagagt ttaatctcaa agcacatatt
     4201 tttttaatta ccggttttat tgggaatggt ccggttcgtc actctcttgg aaaggaatat
     4261 tctcatcgcg attgcgaaca tcaaatagcg acagggaatg ctgataatgt tatgcttcgc
     4321 tggtctgaag taaacgaaat gttgcaatct ggtctggtcg aatttcatgt tcatacgcat
     4381 actcatacac gctgggataa aaagttcagt tcacgagaag aacagtgtaa gcatcttcgg
     4441 caggacttgt tgagcgggag agaatgtttg aaagaaatga caggaaaatg cagtaaacat
     4501 ttgtgttggc cggaaggtta ttataacaag gattatattc aggtcgctga agaacttgga
     4561 ttctattatt tatatacaac agaaagaaga atgaatgctc ctgctaaagg aactacacgg
     4621 ataggaagaa taagcaccaa agaacgggaa agttgcgcat ggctgaaaag acgacttttt
     4681 tattatacaa caccgttttt ttcatcgttg ctggcctttc ataaggggcc aagattaccg
     4741 gatgattaat aatgaatata ctatttacgg aatcatcacc gaatataggt ggtcaggaat
     4801 tacaggctgt tgctcaaatg aaggccctga agaaaatggg gcattcagtt ctgcttgtct
     4861 gcagggaaaa cagcaaaatt gcttttgaag ccagtaaatt gggaattgat atcacattcg
     4921 cgttatttcg aaacagtctt cacatcccta ctgcatggag attactcgga atagttcatg
     4981 gttttcagcc caatgcaatc gtttgtcaca gtgggcatga tagcaatatt gttggtttag
     5041 tacggttatt cactaggaaa catccattca gaattatcag gcaaaagaca tatttgacac
     5101 gaaaaacaaa agttttttca ataaatcatt tttgcgatga ggtgattgtt cccggaacaa
     5161 gtatgaagac acatctggag caggaaggat gtcgaacccg ggttactgtt gtgcctccag
     5221 gctttgattt ccagaaatta tacgttgatt cccgaaacag tttgcctcca aatgttcttt
     5281 cttggctggc gtcccgaagg ggatgccccg ttattgctca ggtaggaatg ttgcgcccgg
     5341 aaaaagggca cgaatttatg ttgaatttac tgttccattt aaaaatgaat ggacgacagt
     5401 tctgttggtt gattgtgggg tctggttcgc ctgaactgcg ggagcattta cagtatcaga
     5461 ttgacagtat gggcatgcat gatgatgttt ttattgctga caatgttttt cctgccgccc
     5521 ccgtatatcg ggttgccagt ctggtggttc tgccttcaga aaacgaatcg tttggtatgg
     5581 tactggcaga agcatcggca ttttctgtgc ctgtactggc cagtcagatt ggtggaatcc
     5641 ctgatgttat tcagaacaac cagaccggga cattgttacc agcaggtaat aagcacgcat
     5701 ggatgtgcgc cctgaatgat ttttttaatg accctgggcg tttttatcag atggctcgcc
     5761 aggcaaaaca ggatatagaa gagcggtttg atattaataa aactgcgtta aaaatactca
     5821 cattagcgaa gcacaagtaa caatatgttt tctgtaagta acttatcatt tatcggtttc
     5881 cttaaaagga ttgttttttc ctcagattca ctcccgggga agtgggaaca cagaaaattc
     5941 cggttcatgt acattttgcg atgtgctata aatccggttg ccagtattcg atattattac
     6001 gaactgcgtt ccttgcagtg cattgaggat attctggcaa tacaacccac gttgccagca
     6061 agaattcatc gaccttattt gcataaaggg gggcgagcct ggtcccgggg gcaatatatt
     6121 cttgagcatt atcgctttgt gcaaaatttg ccagaaaaat attctgaatt tttgtttcca
     6181 caaaaatcgg tatcattagt ccaattcatc gggaaagatg gagaggattt tgatatccag
     6241 tgttctccca gtggttttga ccgggaagga gagttgatgt tgtcattgtt tttcaatcaa
     6301 atagtgattg cccggttaac attttcagtc attctgactc aaaatggaca caccgctttt
     6361 attggcggat tacagggagc ccccaaaaat accggacctg atgttattcg gtgcgcaacc
     6421 cgggcctgtt atgggctttt cccaaaacgg attatttttg aagcattttg tgcgttgatg
     6481 aaagcttgta atgtctctga gtgtctggca gtcagtgagc acagtcatgt tttccggcaa
     6541 ttgagatact ggtatcagaa acgcaaaacc tttgttgctg tctacagtga tttctgggag
     6601 tctgtagcag ggaaaacctg tggtgactgg tacaaattac caacacaggt ggttcgaaag
     6661 ccgctaagca atatagctag taaaaaacgc tctgagtatc gaaaacgata cgctttgctg
     6721 gattatatcc atgaaactgc tatccgctct ttggatgcat atcctgtaaa ctcagaacat
     6781 caggacttaa attaaggttt gatgtattct ctgaataaaa tattaatgat gattttggta
     6841 ggggcattcg cactaaataa tgaaaaaata caaatccgag tttattcctg aatttaagaa
     6901 aaattatctt tcccctgttt actggtttac atggttcgtt ttgggaatga ttgcaggtat
     6961 ttcaatgttt cccccttcat tcagagatcc tgtcttggcc aaaatagggc gttgggtggg
     7021 cagattgagc agaaaagctc gtcgcagggc gacgattaat ttatcgcttt gtttcccgga
     7081 aaagagtgat acagaacggg aaataattgt cgacaatatg tttgccacag cattgcaatc
     7141 catagtgatg atggcagaac tggcaattcg tggtccggaa aagttccaga aacgagtgtt
     7201 ctggaaaggc ctcgaaattc ttgaggaaat ccggcacaat aacagaaatg tgatttttct
     7261 ggttccccat ggctggagcg tggatattcc tgcaatgttg ctggcagctc agggggaaaa
     7321 aatggccgcc atgtttcatc agcaacgaaa tccagtgatt gattatgtct ggaattctgt
     7381 acggcgtaaa ttcggggggc gcttacattc ccgggaggat gggataaaac catttattca
     7441 gtcagtacgc cagggatact gggggtatta ccttccagat caggatcatg gtcctgaata
     7501 cagtgaattt gctgattttt ttgcgaccta taaagcgaca ttaccaatta ttggacgtct
     7561 gatgaacatc agtcaggcta tgattatacc gcttttcccg gtttatgatg aaaaaaaaca
     7621 tttcctgact attgaagttc ggccaccaat ggatgcatgc attgccagcg cggacaataa
     7681 aatgattgcc cgacaaatga acaaaacagt ggaaattttg gtggggtcac atccggaaca
     7741 gtatatctgg gttttaaaat tgttaaaaac gcgcaaatca aacgaagcgg acccgtaccc
     7801 ttgaaattat aaagtacagg tatttccact agttgtttct tacaggttac caatcgaaac
     7861 acatccccct tccgtgtgtt gcccgtttgg cacggaggac tgtgtagatg cagttcgtta
     7921 aatttaattt atg
//
LOCUS       DADMEQ010000150         7929 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.152, whole genome shotgun sequence.
ACCESSION   DADMEQ010000150 DADMEQ010000000
VERSION     DADMEQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.152"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..1153
                     /gene="rbsA"
                     /locus_tag="JNZ05_003552"
     CDS             <1..1153
                     /gene="rbsA"
                     /locus_tag="JNZ05_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HAY8566634.1"
                     /translation="YVEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFESKV
                     IIMDEPTDALTDTETESLFRVIRELKSQGHGIVYISHRMKEIFEICDDVTVFRDGQFI
                     AEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLRKG
                     EILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISEDR
                     KRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQAIG
                     LLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIILVS
                     SEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAVVGKLNRVNQE"
     gene            1158..2123
                     /gene="rbsC"
                     /locus_tag="JNZ05_003553"
     CDS             1158..2123
                     /gene="rbsC"
                     /locus_tag="JNZ05_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY8566635.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2148..3025
                     /gene="rbsB"
                     /locus_tag="JNZ05_003554"
                     /pseudo
     CDS             2148..3025
                     /gene="rbsB"
                     /locus_tag="JNZ05_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3151..4080
                     /gene="rbsK"
                     /locus_tag="JNZ05_003555"
     CDS             3151..4080
                     /gene="rbsK"
                     /locus_tag="JNZ05_003555"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY8566636.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4084..5076
                     /gene="rbsR"
                     /locus_tag="JNZ05_003556"
     CDS             4084..5076
                     /gene="rbsR"
                     /locus_tag="JNZ05_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HAY8566637.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5042..6469)
                     /locus_tag="JNZ05_003557"
     CDS             complement(5042..6469)
                     /locus_tag="JNZ05_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAY8566638.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     NVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6492..7184)
                     /locus_tag="JNZ05_003558"
     CDS             complement(6492..7184)
                     /locus_tag="JNZ05_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAY8566639.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDIAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
     gene            7665..>7929
                     /locus_tag="JNZ05_003559"
     rRNA            7665..>7929
                     /locus_tag="JNZ05_003559"
                     /product="16S ribosomal RNA"
BASE COUNT         2030 a         1901 c         2098 g         1900 t
ORIGIN      
        1 gtatgtcgaa gcggataaat tgctggctaa acttaacctg cgctttaaaa gcgacaagtt
       61 agtgggcgat ctttccatcg gtgaccagca aatggttgaa atcgccaaag tgctgagctt
      121 tgagtcgaaa gtcatcatta tggatgaacc gaccgatgcg ctgaccgata ccgaaaccga
      181 atccctgttc cgcgtcatcc gcgagctgaa atcgcaaggc cacggtattg tctatatctc
      241 ccaccgcatg aaagaaatct tcgagatttg cgatgacgtt accgtttttc gtgatgggca
      301 atttattgct gagcgcgaag tggcatcact gaccgaagat tcgctgattg agatgatggt
      361 gggccgcaag ctggaagatc aatatccaca cctggacaaa gcgccaggag atatccgcct
      421 gaaagtcgat aatctctgcg gacctggcgt taacgatgtc tcttttactt tacgcaaagg
      481 cgaaattctt ggcgtctctg gtttgatggg cgcaggtcgt accgaactga tgaaagtgct
      541 ctacggcgca ctgccgcgca ccagcggtta cgtcaccctg gatgggcatg aagtcgttac
      601 ccgttcaccg caggatggcc tggcaaacgg cattgtgtat atctccgaag accgtaaacg
      661 tgacggttta gtgctgggca tgtcagtaaa agagaacatg tcgctgacag cgctgcgcta
      721 cttcagccgc gctggcggca gtttgaagca tgccgatgaa cagcaggctg tgagtgattt
      781 cattcgtctg tttaatgtga aaacgccgtc gatggaacag gcaattggtc tgctttccgg
      841 tggcaatcag caaaaagtgg cgattgcccg cggtctgatg acacgcccca aagtattgat
      901 ccttgatgag cctacccgtg gcgtagatgt cggcgcgaaa aaagagatct atcaactgat
      961 taaccagttc aaagccgatg gcttgagtat cattctggtg tcatcggaga tgccagaagt
     1021 attaggcatg agcgatcgca tcatcgtcat gcatgaaggg catctcagcg gggaatttac
     1081 tcgtgagcag gccacccagg aagtgttaat ggctgccgtt gtgggcaagc ttaatcgcgt
     1141 gaatcaggag taaaaaaatg acaacccaga ctgtctctgg tcgccgttat ttcacgaaag
     1201 cgtggctgat ggagcagaaa tcgcttatcg ctctgctggt gctgatcgcg attgtctcga
     1261 cgttaagccc gaactttttc accatcaata acttattcaa tattctccag caaacttcgg
     1321 tgaacgccat tatggcggtc gggatgacgc tggtgatcct gacgtcgggc atagacttgt
     1381 cggtaggttc tctgttggcg ctaaccggcg cagttgctgc atctatcgtc ggcattgaag
     1441 tcaatgcgct ggtggctgtc gctgctgctc tcacgttagg tgccgcaatt ggtgcggtaa
     1501 ccggggtgat tgtagcgaaa ggtcgcgtcc aggcgtttat cgctacgctg gttatgatgc
     1561 ttttactgcg cggcgtgacc atggtttata ccaacggtag cccagtgaat accggcttta
     1621 ctgagaacgc cgatctgttt ggctggtttg gtattggtcg tccgctgggc gtaccgacgc
     1681 cagtctggat catggggatt gtcttcctcg cggcctggta catgctgcat cacacgcgtc
     1741 tggggcgtta catctacgcg ctgggcggca acgaagcggc aacgcgtctt tctggtatca
     1801 acgtcaataa aatcaaaatc atcgtctatt ctctttgtgg tctgctggca tcgctggccg
     1861 ggatcattga agtggcgcgt ctctcctctg cacaacccac ggcggggact ggctatgagc
     1921 tggatgctat tgctgcggtg gttctgggcg gtacgagtct ggcgggcgga aaaggtcgca
     1981 ttgttgggac gttgatcggc gcattaattc ttggcttcct taataatgga ttgaatttgt
     2041 taggtgtttc ctcctattac cagatgatcg tcaaagcggt ggtgattttg ctggcggtgc
     2101 tggtagacaa caaaaagcag taataacgac tacaggacat cttgaatatg aacatgaaaa
     2161 aactggctac cctggtttcc gctgttgcgc taagcgccac cgtcagtgcg aatgcgatgg
     2221 caaaagacac catcgcgctg gtggtctcca cgcttaacaa cccgttcttt gtatcgctga
     2281 aagatggcgc gcagaaagag gcggataaac ttggctataa cctggtggtg ctggactccc
     2341 agaacaaccc ggcgaaagag ctggcgaacg tgcaggactt aaccgttcgc ggcaccaaaa
     2401 ttctgctgat taacccgacc gactccgacg cagtgggtaa tgctgtgaag atggctaacc
     2461 aggcgaacat tccggttatc actcttgacc gccaggcaac gaaaggtgaa gtggtgagcc
     2521 acattgcatc tgataacgta ctgggcggca aaatcgccgg tgattacatc gcgaagaaag
     2581 cgggtgaagg tgccaaagtt atcgagctgc aaggcattgc cggtacatcc gcagcccgtg
     2641 aacgtggcga aggcttccag caggccgttg ctgctcacaa gtttaatgtt cttgccagcc
     2701 agcagatttt gatcgcacca aaggtttgaa cgtaatgcag aacctgttga ccgctcatcc
     2761 ggatgttcag gctgtattcg cgcagaatga tgaaatggcg ctgggcgcgc tgcaaactgc
     2821 cggtaaatcg gatgtgatgg tcgtcggatt tgacggtaca ccggatggcg aaaaagcggt
     2881 gaatgatggc aaactagcag cgactatcgc tcagctaccc gatcagattg gcgcgaaagg
     2941 cgttgaaacc gcagataaag tgctgaaagg cgagaaagtt caggctaagt atccggttga
     3001 tctgaaactg gttgttaagc agtagtttta atcaggttgt atgacctgat ggtgacataa
     3061 atacgtcatc gacagatgaa cgtgtaatat aaagaaaagc agggcacgcg ccaccctaac
     3121 acggtggcgc attttatgga catcccgaat atgcaaaacg caggcagcct cgttgttctt
     3181 ggcagcatta atgctgacca cattcttaat cttcaatctt ttcctactcc aggcgaaacc
     3241 gtaaccggta accactatca ggttgcattt ggcggcaaag gcgcgaatca ggctgtggct
     3301 gctgggcgta gcggtgcgaa tatcgcgttt attgcctgta ctggcgatga cagcattggc
     3361 gagagcgttc gccagcagct cgccactgat aacatcgata ttactccggt cagcgtgatc
     3421 aaaggcgaat caacaggtgt ggcgctgatt tttgttaatg gcgaaggtga gaatgtcatc
     3481 ggtattcatg ccggcgctaa tgctgctctt tccccatcgc tggtggaagc gcaacgtgag
     3541 cgtattgcca acgcgtcagc gttattaatg cagctggaat caccactcga aagtgtgatg
     3601 gcagcggcga aaatcgccca tcaaaataag actatcgttg cgcttaaccc ggctccggct
     3661 cgcgaacttc ctgacgaact gctggcgctg gtggacatta ttacgccaaa cgaaacggaa
     3721 gcagaaaagc tcaccggtat tcgtgttgaa aatgatgaag atgcagcgaa ggcggcgcag
     3781 gtactgcatg aaaagggtat ccgtactgta ctgattactt taggaagtcg tggtgtatgg
     3841 gctagcgtga atggtgaagg tcagcgcgtt cctggattcc gggtgcaggc tgtcgatacc
     3901 attgctgccg gagatacctt taacggcgcg ttaatcacgg cgttgctgga agaaaaacca
     3961 ttgccagagg cgattcggtt tgcccatgct gccgctgcga ttgccgtaac acgtaaaggc
     4021 gcacaacctt ccgtaccgtg gcgtgaagag atcgacgcat ttttagacag gcagaggtga
     4081 cgcttggcta caatgaaaga tgttgcccgt ttggcgggcg tttctacctc aacagtttct
     4141 cacgttatca ataaagatcg cttcgtcagt gaagcgatta ccgccaaagt tgaagcggcg
     4201 attaaagaac tcaattacgc gccatcagct ctggcgcgta gcctcaaact caatcaaaca
     4261 cataccattg gcatgttgat cactgccagt accaatcctt tctattcaga actggtgcgt
     4321 ggcgttgaac gcagctgctt cgaacgcggt tatagtctcg tcctttgcaa taccgaaggc
     4381 gatgaacagc ggatgaatcg caatctggaa acgctgatgc aaaaacgcgt tgatggctta
     4441 ctgttactgt gtaccgaaac gcatcagcct tcgcgtgaaa tcatgcaacg ttatccgaca
     4501 gtgcctaccg tgatgatgga ctgggctccg ttcgatggcg acagcgatct tattcaggat
     4561 aactcgttgc tgggcggaga cttagcaacg caatatctga tcgataaagg tcatacccgt
     4621 atcgcctgta ttaccggtcc gctggataaa actccggcgc gcctgcggtt ggaaggttat
     4681 cgggcggcga tgaaacgtgc gggtctcaac attcctgatg gctatgaagt cactggtgat
     4741 tttgaattta acggcgggtt tgacgccatg cgccaactgt tatcacatcc gctgcgtcct
     4801 caggccgtct ttaccggaaa tgacgctatg gctgttggcg tttaccaggc gttatatcag
     4861 gcagagttac aggttccgca ggatatcgcg gtgattggct atgacgatat cgaactggca
     4921 agctttatga cgccaccatt aaccactatc caccaaccga aagatgaact gggggagctg
     4981 gcgattgatg tactcatcca tcggataacc cagccgaccc ttcagcaaca acgattacaa
     5041 cttactccga ttctgatgga acgcggttcg gcatagattt acgctgtctt ttgatcaaat
     5101 tattaccatc ggttgttttc agaagcatga acattgctgc tgaagcaaca gtaataatgc
     5161 ccatcgtgat aaacgtatag tggaattgtt cgacagtcgt tgtgccttcc atcccttcat
     5221 aaatgcgaag gacagccgca cttacagcaa cgcctaaact aatcgacagt tgctgcgtga
     5281 ccgccagaac attgttaccg ctgctggcgt tgtcatcggt cagatcggca agtgtgatgg
     5341 tattcatcgc ggtaaactgc gtcgacatag ccatccctaa tataaacaac ggcaagatca
     5401 gcatccatat cgccattgcc ggtgattgca aagagaactg agcgatcatt agcccaataa
     5461 tcaccgtgat ccccactaat gtatggcgat agcccagacg acgtaagact tgggtaacca
     5521 tcgattttgc aataatggaa cctaacgctg tcggtgccat catacagccg gcaataaacg
     5581 cctgataacc aaatcctacc tgtaacatca atggcataag gaacggtaca cagccggtcc
     5641 ccagacgggt tgcaatattg cctacgatac cgatcgagaa agtgcgggtt ttaaataaat
     5701 ctaatgaaat taatgggttt ggcgtacgtc gcgcatggag aatatagaga agcagtaacc
     5761 cgatgctggt gacaattacc gtcaaggcaa tccagctggc gacaatcttt tccccgaata
     5821 gctctattcc gcttgaaaag agaacaaggc tgaggccaaa cagcaaaaag ccagtgatat
     5881 cgaatctgcg tcgtgcggtg gtgaaattgg gcatatgttt gcgcgcgtaa agaaggcccg
     5941 caatacctat ggggatatta attaaaaata tccagtgcca ggttgcccag gtgaccagca
     6001 cgccgccaag aacggggcct aaaattggcc ccaccagacc cggcatggcg acaaaattca
     6061 ataccggaag aagttcatta cgaggataag cacgcagtaa ggccagccga gcaacaggca
     6121 tcatcattgc gtcgcctatc ccttgaataa cccggaagac aaccagctgt ggtagcgaat
     6181 tagaaagtgc gcaggccaga gaacccaacg tgaacagact cacggcaagg gtaaaaatgc
     6241 gacgcgtacc gaagcgatcg gctagccatc cgcttaccgg aataagcatc gccaccgtca
     6301 gcgtataact gatgatggct gattgcatcg cgagaggaga acgattaagg ctatgagcga
     6361 ttgcgggtaa ggcggtattc agaatagtgg catcaagtgc ctgcatgaaa aaggccatcg
     6421 ccgcgatcca cggcaaaccc gacatactac gcttcttttt atcgctcatt caatgtcctg
     6481 ttatcgggtt atcacttatc aggtgagcgt agcagcgcct gacaagcttt aaatgccgcg
     6541 tcgccatcgc tttggataat cgcatcgaca atcgcctggt gcagatccag ctttatcact
     6601 gtgtcgctgg taattgacgt gaagtaagtg tgataaaccg aatggaatag cgaggcgaat
     6661 gaggtcaaaa acggattggc gctcatttca tagatatgct catgccaggc tatatcgact
     6721 tcgatccagc gttcacggcg aaagttctct tttaatgccg ccatttcggc cattaacgta
     6781 ttgagatgcg ccttctgttc cgcggtgcca accgttgctg ccagtaggca ggcttgcggc
     6841 tccagacaga tacgcataac cagaaagtga tcgatgacct gatgaaagtt ctcttctgtc
     6901 atccaccaag taagcaattc ctgatcaaga aaattccagt tcgattgtgg catgacccga
     6961 gtaccaattc gcggtcgcgg taaaaccatc ccttttgccg ttaacgtttt gaccgcttcg
     7021 cgtaccgctg tacgactcac tccaaattgc tcgcccagct caatctcacc gggcaaaatg
     7081 gtgccgggtt catattcacc ttttaagatc cgttgcgcca gcttctcagc cagaacatac
     7141 gaaaggtttt tctgtgcagc taactgttgt gcgcttaaag gcattactta tcttcctttt
     7201 tctttttatt cctccttagt atgccaccag gaagtgtgat tacggttgca aaaacggcaa
     7261 attgattgtt ttatggcaca ttaacggggc ttttgctgaa aaaatgcgcg gtcagaaaat
     7321 tattttaaat ttcctcttgt caggccggaa taactcccta taatgcgcca ccactgacac
     7381 ggaacaacgg caaacacgcc gccgggtcag cggggttctc ctgagaattc cggcagagaa
     7441 agcaaaaata aatgcttgac tctgtagcgg gaaagcgtat tatgcacacc tcagcgccgc
     7501 tgagaaaaag cgaagcgcac tgctctttaa caatttatca ggcaatctgt gtgggcactc
     7561 gaagatacgg attcttaacg tcgcaagacg aaaagtgaat accaagtctc aagagtgaac
     7621 acgtaattca ttacgaagtt taattctttg agcatcaaac ttttaaattg aagagtttga
     7681 tcatggctca gattgaacgc tggcggcagg cctaacacat gcaagtcgaa cggtaacagg
     7741 aagcagcttg ctgtttcgct gacgagtggc ggacgggtga gtaatgtctg ggaaactgcc
     7801 tgatggaggg ggataactac tggaaacggt agctaatacc gcataacgtc gcaagaccaa
     7861 agagggggac cttcgggcct cttgccatcg gatgtgccca gatgggatta gcttgttggt
     7921 ggggtaacg
//
LOCUS       DADMEQ010000151         7860 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.153, whole genome shotgun sequence.
ACCESSION   DADMEQ010000151 DADMEQ010000000
VERSION     DADMEQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.153"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..165
                     /locus_tag="JNZ05_003560"
     CDS             <1..165
                     /locus_tag="JNZ05_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566640.1"
                     /translation="THYGGAAGPVIFGVGYNDIKSWWLVGAVLTILTFLVHITLGVWW
                     WNMLIGWNML"
     gene            348..2528
                     /locus_tag="JNZ05_003561"
     CDS             348..2528
                     /locus_tag="JNZ05_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HAY8566641.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNHQM"
     gene            complement(2669..3952)
                     /locus_tag="JNZ05_003562"
     CDS             complement(2669..3952)
                     /locus_tag="JNZ05_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HAY8566642.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(4087..5158)
                     /locus_tag="JNZ05_003563"
                     /pseudo
     CDS             complement(4087..5158)
                     /locus_tag="JNZ05_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5136..5354)
                     /locus_tag="JNZ05_003564"
     CDS             complement(5136..5354)
                     /locus_tag="JNZ05_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY8566643.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(5439..6062)
                     /locus_tag="JNZ05_003565"
     CDS             complement(5439..6062)
                     /locus_tag="JNZ05_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HAY8566644.1"
                     /translation="MTSQLIPVFNGTISNETTLLVNARDLHEFLEVRRDFSTWIKNRI
                     TEYGFAVDVDYILVHQNRGIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYTSKLYD
                     IAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF"
     gene            complement(6384..7091)
                     /locus_tag="JNZ05_003566"
     CDS             complement(6384..7091)
                     /locus_tag="JNZ05_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY8566645.1"
                     /translation="MAQLYGTDVVRIRQNHHENKSRFVEGVHFYKVVGDELRALKHKV
                     ALNYFVKSAPNARHLILWTERGAARHAKMLETDQAWDVFEKLEDGYFNQREKVANHPQ
                     PVPQPESSLDRARAMEIALNVADRLVNMFLNLSSESKQAAVAGVFNPIVGRDVVPLPA
                     ISEHYYSATEVGQRYGISANKVGRIANTYMLKTEKYGKWFIDKSAHNDKQVETFRYNE
                     AGVHKIEELIEGERKAA"
     gene            complement(7039..7473)
                     /locus_tag="JNZ05_003567"
     CDS             complement(7039..7473)
                     /locus_tag="JNZ05_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY8566646.1"
                     /translation="MNDKELIKEIKERISSLDVRDNVERRAYEIALASLTAEPVAWKV
                     TFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQPVPVTPDAWISCS
                     ERMPEMGERQCYVLAADFKNNYPPNIGAVVRNGCCAHPAKPS"
     gene            complement(7546..>7860)
                     /locus_tag="JNZ05_003568"
     CDS             complement(7546..>7860)
                     /locus_tag="JNZ05_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001128261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY8566647.1"
                     /translation="VQAYANAEFIAAFNPNVALALLDERERNLQYIKSRDQENEEIAL
                     TVGKLRVELEAAENNLIDSECHVAELEEALRDKQALLEASEKRIAELEAQTVTVKEVG
                     DA"
BASE COUNT         1813 a         1957 c         1926 g         2164 t
ORIGIN      
        1 actcactatg gcggcgcggc aggtccggtc atctttggcg tgggttacaa cgatattaaa
       61 tcctggtggt tggtcggtgc ggtactgacg atattaacct tcttggtgca tatcaccctc
      121 ggcgtgtggt ggtggaatat gctgatcggc tggaacatgc tgtaaatata ctcgtcatac
      181 ttcaagttgc atgtgctgcg tctgcgttcg ctcaccccag tcacttactt atgtaagctc
      241 ctggggattc actcacttgt cgccttcctg caactcgaat tatttagagt atatccattt
      301 attatctttc tgcgcacttc acggtgcgca gatatctgga gcatttgatg atcaagttat
      361 ctgaaaaagg cgtgtttctc gccagtaata acgaaataat tgccgaagaa catttcaccg
      421 gcgaaattaa aaaagaagaa gcaaaaaaag gcactattgc ctggtctatt ctctcttctc
      481 ataatacgtc cggaaatatg gataaactta aaattaagtt tgattcatta gcctctcacg
      541 atattacctt tgttggtatt gtacagaccg ctaaagcgtc cggaatggaa cgtttcccgc
      601 tgccgtatgt gctgaccaac tgccataact cactctgcgc cgtcggcggc accattaacg
      661 gtgatgacca tgtttttggt ttatcggcag ctcagcgtta tggcggtatt tttgtgcctc
      721 cgcatattgc ggtcatccat caatatatgc gtgagatgat ggctggcggg ggcaaaatga
      781 tcctcgggtc agacagccac acccgttacg gtgcattagg gacaatggca gtcggtgagg
      841 gtggcggtga gttggtaaaa cagctgctta atgacacctg ggatatcgac tatccgggcg
      901 tggttgcggt gcatctgacc ggaaaacctg cgccgtatgt ggggccacag gatgtggcgc
      961 tggctatcat tggcgcggtg ttcaaaaacg gttacgtcaa aaacaaagtc atggagttcg
     1021 ttggaccggg cgttagcgcg ctctctaccg atttccgtaa cagcgttgac gtgatgacca
     1081 cggaaaccac ctgtttaagc tccgtctggc aaactgatga agaagtgcat aactggctgg
     1141 cgctgcacgg tcgcggccag gattactgcc agcttaaccc tcaaccgatg gcgtactacg
     1201 atggctgcat cagcgttgat ttaagcgcca tcaaaccaat gattgcgctg ccgttccacc
     1261 cgagcaacgt gtatgaaatc gacacactga accagaacct gaccgacatt ctgcgtgaga
     1321 ttgaaattga gtccgaacgc gtggcgcacg gtaaagccaa actctcgctg ctggataaag
     1381 tggaaaatgg tcgcctgaaa gtgcagcagg ggattatcgc gggctgttct ggcggtaact
     1441 acgaaaacgt catcgcggcg gcgaatgcac tgcgcggtca atcctgtggc aatgacacct
     1501 tctcgctggc ggtttacccg tcatcacagc cggtgtttat ggatctagcc aaaaaaggtg
     1561 tggtagcaga tttgattggc gcaggcgcaa tcatcagaac cgcattctgt ggcccatgct
     1621 ttggcgcggg cgatacgcca atcaacaacg gtttgagtat tcgccacacc acgcgtaact
     1681 tcccgaaccg cgaaggctct aagccagcca atgggcagat gtcagcggtg gcgctgatgg
     1741 acgctcgttc tatcgctgcg actgcggcaa acggtggcta tttaacctct gccagcgaac
     1801 ttgattgctg ggacaacgtg ccggagtacg ccttcgatgt aacgccgtat aaaaaccgtg
     1861 tttatcaggg ctttgtgaaa ggggcaactc agcaaccgct gatttacgga ccgaacatta
     1921 aagactggcc ggaattgggt gcgctgactg acaatatcgt cctgaaagtg tgctcgaaga
     1981 tcctcgacga agtcaccacc accgacgaac tgattccttc cggtgaaacc tcttcttatc
     2041 gttcaaatcc gattggtctg gcggggaatg tcagcgagct gacagaggtg tttgcgcgca
     2101 ttaagcagat tgctggtcag gagcatattg atccgctgca aactgaaatt ggcagcatgg
     2161 tctatgcggt gaaaccaggc gatggttctg cgcgtgaaca ggcggcgagc tgccagcgtg
     2221 tgattggcgg tctggcgaat attgccgagg agtacgcgac taaacgctac cgttctaacg
     2281 tcatcaactg ggggatgtta ccgctgcaga tggcggaagt gccaaccttt gaagtggggg
     2341 attacattta catccctggc attaaagcgg cgctggataa tccgggtacg acgtttaaag
     2401 gttatgtgat ccatgaagat gcgccggtaa cggaaattac gctttacatg gaaagcctga
     2461 cggctgaaga gcgcgagatt atcaaggcgg gtagtttgat taacttcaat aaaaaccatc
     2521 agatgtaaaa agcgccatgt gaatgtaggt cgcattcggc acttattgtc ggatgcgatg
     2581 ctggcgcatc ttatccgacc tacaaatcgc atcgaaccgt aggccggata aggcgtttac
     2641 gccgcatccg gcaaatagtt aatagctctt acttcttcgc ctctgcaacc actttgctgc
     2701 ccacgccgcg gttattgtat tcccacatgc ggttgtagtt agtgtcattc agattgcgct
     2761 gtatttcgtc gttatcatct acgctgccgg tattacccgc aaacggacga ttagagatca
     2821 ccgcatcggc ccacggttta gccgtgttaa aaccttcgtt gatggcgcta tcacggatca
     2881 ccacctgacc gttggtatta gcatcaacat ccagcgagcg gcccagttgc gccacgccat
     2941 caccggaagc attgaaacgg ctgtttacgg cgaggaaacc gtaatagatg ttagacagcg
     3001 tagccggtgc aaacacatat gcttcttgct gggtacgtga gttcaccacg cggaattcgg
     3061 tgttatcgaa caccactgcg ccgcgaccag aaacgatatc cacatcccct tcaatatagc
     3121 tgttggtcac cagcgtacgc ggctgacggt tggtttccag acggttctgc acaccgctgt
     3181 tggtgacaaa gaaggtgttc tgacgaccga gaatgttaac gttgttaatc tgtacctggt
     3241 caccgtcagt acgcagtgct accgccggat ggttacctgc atctacacta tcgcccagcg
     3301 tgttttcgat ggtcagattt tgcagttgca gtccattgtt ttgtgaccag aagaccgcag
     3361 aacacagaac accgatactg tcgctgcgtt tgctctggca gctatcgtac atataccacg
     3421 ctggtttacc tggcatatat ttgccgcgcg ggttgacgtc gtgacgccag tcggcagggc
     3481 tcatgccccc atcaagggaa agcccaatct tcacatcaat cggtttttca cctgtaccgt
     3541 acagagtgat tccacctgga gcagcaggga catacaccgt tccctgatac tcaccaggca
     3601 tcacggcaat atactggcgc ttgttggtgc gcttgataat tgccgcatct accgccgcct
     3661 gaatcgtggt atgcgttaca ccttgagtac ccgccgggcc gacaacaaag tcaggttgcg
     3721 caggcagggt tatcggggac ggattccacg ctgcagcacc tggtgtcagg gatgcaaaat
     3781 agtgttgcgc atcgaaattc agcgcttctt ttgccgacag aatcgggcga gaagaggtac
     3841 caggcgcagt ttgatcagaa ggacgttgat cgggtggtgt tgagctacag gcggtcagcg
     3901 tcacgccaaa agccaatgcc agcgccagac gggaaactga aaatgtgttc acaggttgct
     3961 ccgggctatg aaatagaaaa atgaatccgt tgaagcctgc ttttttatac taagttggca
     4021 ttataaaaaa gcattgctta tcaatttgtt gcaacgaaca ggtcattatc agtcaaaata
     4081 aaatcattat ttgatttcaa ttttgtccca ctccctgcct ctgtcatcac gatactgtga
     4141 tgccatggtg tccgacttgt gcccgagaag atgttgagca aacttatcgc ttatctgctt
     4201 ctcatagagt cttgcagaca aactgcgcaa ctcgtgaaag gtaggtggat ccccttcgaa
     4261 ggaaagacct gatgcttttc gtgcgcgcat aaaatacctt gatactgtgc cggatgaaag
     4321 cggttcacga cgagtagatg caattatggt ttctccgcca agaatctctt tgcatttatc
     4381 aatgtgtttc cttcattgat atcccgagag catcaacatg caatgttgta gggatggcaa
     4441 tttttacgcc tgttttgctt tgctcgacat aaagatatcc atctacgata tcagaccact
     4501 tcatttcgca taaatcacca actcgttgcc cggtaacaac agccagttcc attgcaagtc
     4561 tgagccaaca tggtgatgat tctgctgctt gataaatttt caggtattcg tcagccgtaa
     4621 gtcttgatct ccttacctct gattttgctg cgcgagtggc agcgaccggg tttgttgtta
     4681 tatggccttc agctattgcc tctcggaatg catcgctcag tgttgatctg attaacttgg
     4741 ctgatgccgc cttgccctcg tctatgtatc cattgagcat tgccgcaatt tcttttgtgg
     4801 tgatgtcttc aagtggagca tcaggcagcc ccctccttat tgctttaatt ttgctcatgt
     4861 aatttatgag tgtcttctgc ttgattcctc tgctggcgaa gattttttcg tagcgatcaa
     4921 gccatgaatg taacgtaaca gaattatcac tgttgattct cgctgtcaga ggcttgtgtt
     4981 tgtgtcctga aaataactca atgttggcct gtatagcttc agtgattgcg attcgcctgt
     5041 ctcggcctaa tccaaactct ttacccgtcc ttgggtccct gtagcagtaa tatccattgt
     5101 ttcttatata aaggttaggg ggtaaatccc ggcgctcatg acttcgcctt cttcccattt
     5161 ctgatcctct tcaaaaggct acctgttact ggtcgattta agtcaacctt taccgctgat
     5221 tcgtggaaca aatactctct tccatcctta accggaggag ggaatatcct gcactcgcgt
     5281 acccatcgac gaactgtttc aaggctcctt gggcgtcgct ggcgtgcgtt ccactcctga
     5341 agtgtcaagt acatcgcaaa gtctccgcaa ttactcgcaa gggagcgagt attggtttat
     5401 tttctccaac aaaaaaggag ccgaagctcc tttgatgatt aaaattcgaa ttgtctcgcc
     5461 cgaaggcttt tcagcattgg ttttgcccgt tcgaaaaggg cgcttgcttg gtcaagtcgt
     5521 gtcgcctccc ttagcagtac atctctgttt ttcgtcaccg tgtaaaaagt ttcaaacgcg
     5581 atgtcataca gcttgctcgt gtatgatgag ttcagttcct tcaatatcgg gtacagacgt
     5641 ttgcagatgt cctgcgcatt ctccatctgt acctgcatat agcaaaggag gatgatttct
     5701 tcgtctgtga attgctgcgc tggttgcatg ctgcgaagtt tcttttcgca ctcgatgaag
     5761 tatcggcgta tctggcggcc tttttcgtta cgctcgacca tcgccgtttc tttggctgta
     5821 tcgagggtga ggtgatagtc tttgctacgg cgatcgcctc cacgtccttt gatccccctg
     5881 ttttggtgaa ccaaaatgta atcgacatca acggcaaagc cgtattctgt tatgcgattt
     5941 ttaatccatg tagagaagtc acgacgtact tccaaaaatt cgtgtaaatc acgggcatta
     6001 acgagaagag ttgtttcgtt ggatatagtg ccgttgaata cggggatgag ttgactggtc
     6061 atttttatgt cctttgcatt tgttcagata gccctgtgtt cagcagggcg gtcaggtact
     6121 tgaacaccgt gcaaagtcgg cccgtatcct taacctttcg gctgtttttc ggtatacgcg
     6181 ctacccgacc atatctgaaa aatggacata aaaaatccgc atgactgacg ggtgcggttt
     6241 ccgctttgca aggtgtgttc agcaccatga agcggaatat agccccgtta atgcggtgtt
     6301 gtcaaatcat gtaggcctta tcttgctgta agccgcgcca ttcgtgcttt tccccacatt
     6361 tggggaaaac tatcttgtca aaatcatgca gcctttcgct ctccttcgat gagttcctct
     6421 attttgtgca ctccagcctc gttgtaccga aatgtttcaa cctgcttgtc attgtgcgcc
     6481 gatttgtcga tgaaccactt cccgtacttt tctgttttga gcatgtaggt gttggctatg
     6541 cgtcctacct tgttggctga aataccgtag cgctggccga cttccgtagc agagtagtag
     6601 tgctctgaga tagctggcag aggtacaaca tcgcgaccaa caatcgggtt gaacactcca
     6661 gctactgctg cctgctttga ttctgaggac agattcagga acatattcac cagtcgatcc
     6721 gccacgttca aagctatttc catggctcgc gcacggtcta ggctactttc tggttgcggt
     6781 actggttgag gatggttggc aaccttttcg cgctgattga agtagccgtc ctccagtttt
     6841 tcgaacacgt cccatgcctg atccgtttcg agcatcttcg cgtgacgggc ggctccgcgc
     6901 tctgtccaga ggatgaggtg gcgtgcattt ggggcacttt tcacaaagta gtttaaagct
     6961 actttgtgtt ttagcgcgcg cagctcgtca ccaacgacct tgtagaagtg taccccctca
     7021 acaaagcgcg acttgttttc atgatggttt tgccggatgc gcacaacatc cgttccgtac
     7081 aactgcgcca atgtttggtg ggtagttgtt tttaaagtca gctgctaaca catagcattg
     7141 tcgctctccc atttctggca ttcgctcact acagcttatc caggcatccg gagttaccgg
     7201 cactggctgg gcggtataaa gcggtgttat atctgcccga aaattacatg ctttatgcag
     7261 ccgcacccac cgttcgactt ctgctttgtc agaatacata ccagtgaacg tgttatattc
     7321 acggtcaatt tgcgtgaagg ttaccttcca cgccaccggc tctgctgtca gcgatgccag
     7381 agcaatttca taagcacggc gttcaacatt gtctcgcaca tccaaactgc tgatacgctc
     7441 tttgatttct ttaatcagtt ctttgtcgtt cacttatgcc tccgattccc ggatgtcacc
     7501 caaatcaacg ctacaatgcc aaccagtgca acgagcgcaa aactatcaag catctccaac
     7561 ctctttaacg gtaactgtct gtgcctccag ttctgctatg cgcttttctg aggcttcaag
     7621 taacgcctgc ttatcgcgta gcgcttcttc cagttcagca acatggcatt cactatcaat
     7681 aaggttgttc tctgctgctt ccagctcaac acgcaacttc cctaccgtta gcgcaatttc
     7741 ctcgttctcc tgatcgcggc ttttgatgta ttgcaggttt ctttcccgtt catccagtag
     7801 tgccagcgca acatttggat taaaagcagc aataaattca gcgtttgcat aagcctgaac
//
LOCUS       DADMEQ010000152         7819 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.154, whole genome shotgun sequence.
ACCESSION   DADMEQ010000152 DADMEQ010000000
VERSION     DADMEQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.154"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            211..429
                     /locus_tag="JNZ05_003569"
     CDS             211..429
                     /locus_tag="JNZ05_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HAY8566648.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            647..889
                     /locus_tag="JNZ05_003570"
     CDS             647..889
                     /locus_tag="JNZ05_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566649.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1219..2148)
                     /gene="fdhE"
                     /locus_tag="JNZ05_003571"
     CDS             complement(1219..2148)
                     /gene="fdhE"
                     /locus_tag="JNZ05_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HAY8566650.1"
                     /translation="MSIRIIPQDELGSSEKRTANMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2145..2780)
                     /gene="fdoI"
                     /locus_tag="JNZ05_003572"
     CDS             complement(2145..2780)
                     /gene="fdoI"
                     /locus_tag="JNZ05_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY8566651.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2777..3679)
                     /gene="fdxH"
                     /locus_tag="JNZ05_003573"
     CDS             complement(2777..3679)
                     /gene="fdxH"
                     /locus_tag="JNZ05_003573"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY8566652.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3692..6742)
                     /gene="fdnG"
                     /locus_tag="JNZ05_003574"
     CDS             complement(3692..6742)
                     /gene="fdnG"
                     /locus_tag="JNZ05_003574"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6155..6157),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY8566653.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLSDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6936..7769
                     /gene="fdhD"
                     /locus_tag="JNZ05_003575"
     CDS             6936..7769
                     /gene="fdhD"
                     /locus_tag="JNZ05_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HAY8566654.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1814 a         2012 c         2076 g         1917 t
ORIGIN      
        1 aatataatat attcatagca cccataactc atcctgggcg ataactaaac catttttgag
       61 taataaatga aacaactgca acgctcttat tatttctgga aaacgcctcg caaaaacaaa
      121 atgacatgca aaaatccgca tttttttaaa tgctttttcg gcgtaatttt cgcacataca
      181 ttaatgagta atatatgtaa ggtggtggta atggcgatga atacggtttt tcttcattta
      241 tcagaagagg caattaaacg gctgaacaag cttcggggct ggcgtaaggt ttcacgatct
      301 gcaattttac gcgaagcggt tgagcaatat ctggagcggc agcaatttcc ggtgcgtaaa
      361 gcaaagggag gcaggcaaaa gggggaggta gttggtgttg atgatcagtg taaggagcat
      421 aaggaatgaa aaaaattttt cattcttgaa tataaaaaac agatgccctt attctggtat
      481 taatacaagg ctgttttact tgaacttata ataactgcaa ctgttacatc gtatctggaa
      541 aacgcctcgc aaaaataaaa aatgatgcgt aaatgagctt tctttaaaga ttcggcaagg
      601 gtacttttct ctccatagat tcaaaaatga tattggcgag atatttatga actctctggc
      661 aggtatagat atgggcagaa ttttactcga tttatcgaat gaggtgatta agcaacttga
      721 tgatctcgag gtgcagcgta atcttcctcg tgcggaccta ttaagggaag cggtagatca
      781 atacctgata aatcaatcgc aaacagcaag aaccagtgtt cctggcatct ggcaagggtg
      841 tgaggaagat ggtgtcgaat atcagcgtaa gctgcgcgag gaatggtaat gggaaaaaat
      901 gatgtcaatc agattgctga caacgtgcgc gttgttcatg ccggatgcgg cgtgaacgcc
      961 ttatccggcc tacaaagtca tgcaaattca atatattgaa gtttattggt aggcctgata
     1021 agcgcagcgc atcaggcaat catacgcttg tcatcagtga catacccata accttatcga
     1081 tgtgttggag ggaagaacga ggcaaacaga caattgcggc atgatcttga atgggtaacg
     1141 cggaatacga gagatggtgt cgtcacaccc tatgagctgt aatccgggcg acctgcgcca
     1201 cccggaaaac gtcagagatt actctccttc acccggaaac aggaacgggt taatggaact
     1261 acgggcgtag ccttcttgct ccattcgcgc gtccagtacc agagaggcga ggtcatctgc
     1321 cacggcttca actttcggtt ctttttcctg ataaagaatc ttcaggtaag tgccgcagtc
     1381 atcgcagctt tcggctttaa tcgcggcctg ttcgtcatcc agcgaccagt aatgcagttt
     1441 gccgctctgc tcgcagttgc tgcattttac gcgcactacg tgccattcgg tttcacacag
     1501 gttgcagtgc aggtaacgca gaccctgagt ggtgccaatt tgcaccatgc tggacaccgg
     1561 catagagcca cataccgggc aatattgacg ttgttcgccg tattcagcgc gggctttgcc
     1621 ggggatcaga ttggccatct gcgcccagta gagcgacagc gcagcccaga taaacggcgc
     1681 tttatcgctg ctgacggacg agaaatcaga ggcaaacagc gcgctggcca tatcttccag
     1741 ctcctgagtc gatgccttct ccagattctc aatcactgcc agcgccgggc cgctcatttc
     1801 aggtttcagc tcagcaatta gcgccatcag cagcttttgc cagtgcttat cacgcggcag
     1861 aacgtgaata tccagcgggg gcttgccctg tgcgctggct tctttaatgc gcgcggtcag
     1921 atccatctcc agcggatggt cgtacagcac cacttcctgg gcgtgggcga taagcgcagc
     1981 aaagcgcagg taatcaccca gcggattatt ttctgccagc tcgcgcagac gctcggcgcg
     2041 gcggttgtat aaattcttga gccgagggaa caataacggc ggaatcatat tcgccgtacg
     2101 tttctcgctc gaacccagct catcttgcgg gattatgcga atactcattc agctttcttt
     2161 tccgttgtct tgcggacctc acggtaccag cgcgggtgat gtttcttcgc ccaggcgctg
     2221 gtaacccatc cttccaccat ggcggtaatc gtgcctttca cccaaagggc ggcgtagata
     2281 tgcaccatga taaccacaat taacgccact gcggcaaatg aatgcagcat taacgcgaat
     2341 cggatcaccg ggattgagaa agcaggcgca aaataaggac gccagataat cacgccgctc
     2401 accagcagca gaaccaggaa aataatcgcc gcccagaaaa cgcatttctg accgaagtta
     2461 taacgcccgg tgtcacctac ttcctcgttg acgacgatct tacgaatatt cttcgcccaa
     2521 aagatatcat cccgattgat taggttttga tgccagtaac ggaaaaacat gatgatgaac
     2581 gaggcaaaca taaccacgcc gacaaacggg tgcagaattc gcgccagctg cggtgtgccc
     2641 atgatttgca tcaaccagtt gaaggacggg aacaaaaagc ccagcccgct caccgccgcc
     2701 aggatgaagc agaaggcggt gatccagtgg ttgatacgtt ccggcgcggt gtagcgcacg
     2761 atggtgtcac gtcgtttcat ttgcgctcct cgtctttctc ttcgtgcaga ttattctctt
     2821 cctcatccgc acggttcgga ccgacaccca cgtagtggaa gatactggcc gcaaaggtag
     2881 ccgcaaagcc aacagccgcg agcggtttcc agatgccttt ccagaatttc acggtttcgc
     2941 tgatttccgg gttctccggc aagccatgat acagatttgg cttgtcagca tggtgcagca
     3001 cgtacatgac gtgtgtacca ccgacgcctg ccggatcgta cagacccgca ttgtcgtaac
     3061 cgcgggtttt cagctcagca acgcgctcgc tcgccagcgt tttcatcgac tctttcgtac
     3121 cgaagtgaat cgcgcccgtt gggcaggtct tcacgcaggc tggttcttgc ccaaccacca
     3181 cgcggtcaac gcacagcgta catttgtaga cgcggttgtc ttccgggttg aggcgcggaa
     3241 tgtcgaacgg acagcccgca atgcaataac cgcagccaat gcactgctcg gactggaagt
     3301 cgacgatacc gttggcatac tgaataattg caccttccgc cgggcacgct ttcaggcagc
     3361 ctggatcgga acagtgcata cagccgtctt tgcggatcag ccattccagt ttgtcgttct
     3421 gctccacttc cgagaagcgc attaccgtcc acgatttggc gcttaaatca ttggggttgt
     3481 cgtacacccc aatgttattg ccgacggtat cgcgaatgtc gttccactct gaacacgcca
     3541 cctgacaggc tttacagccg atacaggtgg taacgtcgat gagtttcgcc acttcttcct
     3601 ggaagtcccg cgcctgaggc gcgggggtca gaccgttagt cgcggaacga cgaatgatat
     3661 cttgagattg ataagccata tgtcgtctcc gttacacctt ttccacattc acaaggaagg
     3721 acttaaactc cggcgtctgc gtgttcgcat caccaacgaa tggcgttaaa gtattggcaa
     3781 taaagccttt tttagctacg ccttcatagc cccagtgaat cggaataccg atggtatcga
     3841 tatctttgcc gttcgctttc agcgtgcgaa tacgtttggt caccaccgct ttggctttga
     3901 tatagccacg gttggaggag actttcacgg tatcgccttg ggcaatgcca agtttattcg
     3961 ccagcgactc cccgatttcc acaaactgct ctggctgcaa aatcgcgttc aacagcgcgt
     4021 gtttggtcca gtagtggaag tgctcggtca gacgataggt ggttccgaca tacgggaact
     4081 tatcggcttt acccaatgct tcggcgtcgt ctttaaagat acgtgcagcc gggttcgaga
     4141 taacgtttgg atgcagcggg ttagttccca gcggcgtttc aaacggctcg tagtgttccg
     4201 ggaacggacc ttccgccatc ttatcgaggg caaacagacg ccccatgcct tcctgctgca
     4261 tgataaacgg cccgacgccg ctacccggag gcgctgcgct gtagtccgga atatcccagc
     4321 cggtccactt agtgccgtcc catttcagca actgacgctt cggatcccac gggttaccct
     4381 gcggatctgc ggaggcgcgg ttatacagaa tgcggcggtt aagcggccat gcccatgccc
     4441 agcccagcgt gttaccgagg ccagacggat cggcgttatc acgacgcgcc atctggttgc
     4501 cttccggcgt ccagctaccg gcgaaaatcc agcagccaca ggaggttgta ccgtcatcgc
     4561 gcaattgggc gaacgagcta agttgttggc ctttcttgac gataaccgca ccggttgccg
     4621 gatcggtaat atcggccagc gccttaccgt tgctctccat cgctacttct tccgacttcg
     4681 gctcatgcgg aatggcgtag ttccaggtca tgttcagcac ctgatccggg ttcgcgccac
     4741 cctgttcggc atacatcttg cgcaagcgca ggaagatacc ggagaggatc tcaccgtcag
     4801 tcagcgcaat ccccggggcg tccgcacctt tccagtgcca ctgcaaccag cgacctgagt
     4861 taacgattga accgttctct tctgcgaagc aggtcgatgg cagacggaac acttcggtct
     4921 ggatcttcga cgagtcaact tcgttcagct caccgtggtt ctgccagaag ttagaggttt
     4981 cagtgttcag tgggtcgatg gtgacgagga acttcagttt cgacagacaa ccgatcactt
     5041 tgtttttgtt cgggaatgag gcaacagggt taaagccctg gcagatatag ccattgacct
     5101 tgccctcttt catcatctcg aagtattgca ggacgtcgta gcctttatcc cacttcggca
     5161 accagtcaaa gccccagcta ttttccgccg tcgctttatc accaaagaag gccttcatca
     5221 tagagacgaa gaatttcggg tagttgcccc agtagtttac ctggccttcc agcagcggtt
     5281 ttggcgtgtt ggcggtaagg taggtttgca gatcggtctg cttctcgctt ggcagcgtca
     5341 tgtaacctgg cagactctgc gacagtagcc ccaggtccgt cagcccctga atattggagt
     5401 gaccgcgcag ggcgttaacg ccgccgccgg ccatccccat attgccgagc agcagctgga
     5461 tcatcgccat cgtacgaatg ttttgcgcac caacggagtg ttgcgtccag ccgagggcat
     5521 acaggaacga ggcagtttta tcgtgagcac tggtttctgc gatgtattcg cagactttca
     5581 ggaacgcgtc ttttggcgta ccacagatgt tttcaaccac atctggcgtg tagcgggaaa
     5641 cgtgctgttt cagcaagttc cacacgcagc gcggatgttg cagcgtggta tcgcgtttgg
     5701 cgaagccgtt ttcgtccagt tcataagtcc aggaggattt atcgtacttg cgtttttccg
     5761 cgtcgtagcc ggtgaacagg ccatcttcaa agccgtaatc ctcacgcacg atcaggctgg
     5821 cgttggtata ggcttcggtg tattcgtggt tgaatttttc attgttcagc aggtacagca
     5881 atacgcctga caggaaagca atgtcagtac cggaacgaat aggggcatag tagtcagcta
     5941 ccgccgccgt acgcgtaaag cgaggatcga tcacaatcag cttcgcgccg ttgtgaattt
     6001 tggcttccat cgcccagcgg aacccgaccg ggtgagcttc agcggcgtta ccgcccatca
     6061 ccacgacgag gttggcgttc ttgatgtcga cccagtggtt ggtcatcgca ccgcgaccaa
     6121 atgttggagc aagacttgct accgttggtc cgtgtcagac acgcgcctgg ttgtcgaccg
     6181 cgagcatacc cagcgcgcgg gagaattttt gcgttaaata gccggtttcg ttactcgacg
     6241 cggaagcaca cagcatcccg gtggagagcc agcggttaac agtcacgcct tcggcgtttt
     6301 gcgcaatgta gttagcatcg cggtcttctt tcatcagttt ggcgatgcga tcaaacgcct
     6361 cttcccaact gatttgctgc catttatcag aacctggcgc acggtattcc ggaaacttca
     6421 gacggctttc ggagtggatg aaatccacca ggccagcgcc tttcggacaa agtgcaccgc
     6481 ggttgaccgg gtgatccgga tcgccttcga tatggaagat agatgctttg gcgttttttg
     6541 ctccgtcact gaggctgtac atcaacagcc cacagcctac ggaacaatag gtgcaggtat
     6601 tacgggtttc gcgggtgcgc agcagtttat actgccgggt ttccgcgagt gctacgctgg
     6661 gtgcaaaacc cagtgccgct gccgtggtgc ctgccatacc gccagcgcag atcttaaaga
     6721 actgccttct gctgacctgc atggattgct ccttgtttcg acattgtcac gtcccattta
     6781 cattcgcttg ctgcgtgtgc agggagtggg agttattttt ctttgcggaa ggggccgcaa
     6841 aggtccagaa ttggctcaat ttccctccat ctaggaaggg tttgtaacag aataccataa
     6901 tgttggtgtg tgtgttctta tctggttaag agaaagtgaa aaaaacacag cgaaaagaaa
     6961 tcgaaaatgt gacaaatatc acaggtgttc gtcaaattga gttatggcgg cgtgatgatt
     7021 tacaacaccc acggttagat gaggtcgcgg aagaagttcc cgttgcgctg gtctacaacg
     7081 gcatttcgca tgtggtgatg atggcgtcgc ccaaagacct tgagtacttt gcgctcggtt
     7141 tttcgctttc cgaggggata atagaaagtc cgcgcgatat cttcggcatg gatgtcgttc
     7201 cttcctgtaa tggtcttgaa gtacaaattg agctttccag ccgccgcttt atggggttga
     7261 aggagcgccg ccgggcgctg gcgggacgta cgggctgtgg cgtatgcggc gtggagcaac
     7321 ttaatgacat cggaaaaccg gtgcagccgc taccgttcac ccagacgttt gatctcaaca
     7381 aactggatga tgcattacgt catctcaacg attttcagcc agtggggcaa ctgactggtt
     7441 gtactcacgc cgctgcctgg atgttgccat ctggcgaact ggtcggcggg catgaagacg
     7501 tgggtcgcca tgtggcgctg gacaaactgt taggccgccg gtcacaagaa ggggaaagct
     7561 ggcagcaagg tgcggtactg gtttccagtc gtgccagtta tgaaatggtg caaaagtcgg
     7621 cgatgtgcgg cgtagagatt ttgtttgcgg tgtctgccgc gaccacgctt gctgtagaag
     7681 tggctgagcg ctgtaatctg acactggtag gtttttgtaa accgggtcgg gcaacggttt
     7741 atacccatcc gcagcgtttg agcaattaat taaaataagt cctgcgatat atattaaatt
     7801 gcaggactta ttcatttcg
//
LOCUS       DADMEQ010000153         7753 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.155, whole genome shotgun sequence.
ACCESSION   DADMEQ010000153 DADMEQ010000000
VERSION     DADMEQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.155"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(1..414)
                     /locus_tag="JNZ05_003576"
                     /pseudo
     CDS             complement(1..414)
                     /locus_tag="JNZ05_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(754..1308)
                     /locus_tag="JNZ05_003577"
                     /pseudo
     CDS             complement(754..1308)
                     /locus_tag="JNZ05_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1451..1573
                     /locus_tag="JNZ05_003578"
                     /pseudo
     CDS             1451..1573
                     /locus_tag="JNZ05_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1835..2401)
                     /locus_tag="JNZ05_003579"
     CDS             complement(1835..2401)
                     /locus_tag="JNZ05_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566655.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2683..2997)
                     /locus_tag="JNZ05_003580"
     CDS             complement(2683..2997)
                     /locus_tag="JNZ05_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566656.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3075..3185)
                     /locus_tag="JNZ05_003581"
                     /pseudo
     CDS             complement(3075..3185)
                     /locus_tag="JNZ05_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3509..4186)
                     /gene="ospD1"
                     /locus_tag="JNZ05_003582"
     CDS             complement(3509..4186)
                     /gene="ospD1"
                     /locus_tag="JNZ05_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspD1"
                     /protein_id="HAY8566657.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNSHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4557..4694)
                     /locus_tag="JNZ05_003583"
                     /pseudo
     CDS             complement(4557..4694)
                     /locus_tag="JNZ05_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4776..5074)
                     /locus_tag="JNZ05_003584"
                     /pseudo
     CDS             complement(4776..5074)
                     /locus_tag="JNZ05_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5663..5822)
                     /locus_tag="JNZ05_003585"
                     /pseudo
     CDS             complement(5663..5822)
                     /locus_tag="JNZ05_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5826..7055)
                     /locus_tag="JNZ05_003586"
                     /pseudo
     CDS             complement(5826..7055)
                     /locus_tag="JNZ05_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(7075..7422)
                     /gene="tnpB"
                     /locus_tag="JNZ05_003587"
     CDS             complement(7075..7422)
                     /gene="tnpB"
                     /locus_tag="JNZ05_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY8566658.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(7422..>7753)
                     /locus_tag="JNZ05_003588"
     CDS             complement(7422..>7753)
                     /locus_tag="JNZ05_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8566659.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQILIR
                     EIKGSSH"
BASE COUNT         1992 a         1805 c         1694 g         2262 t
ORIGIN      
        1 tggatctgat ttcaggcgtt gggtgtggat cactattgca ccgttcatga cagtgttgtt
       61 gcgccttgct gccaacgaag ctccactgcc catcaagttc acagataagg gcaacatcag
      121 cgtgagcgac tggcgcagat gttatccgcc ggggcgggag ctttttaatg tccgtatgac
      181 ggtattgatg ccgattttca gcgtacgggc agtatcgcga actccggcac cgttgaaagc
      241 catttcagtg atttggtttt taactcccgg tttacgggcc tcgtaacagt aagtgagctg
      301 aaacacccgg tgacagtcac ggcaacggaa tcgatcatgt ccttttgggt tctgcccgtg
      361 acggtaaacc tgaacggact ggcaacgggg gcaatgaaag ttaactgtga ccatgaaaga
      421 accttaaaag cgggtattat aacccctaat tcaactaatt ggaggcattg tcatttttaa
      481 tctatggata cacttaaaaa tcctctcacc ttacgttaaa aaccttctca tgtttagagt
      541 tttttattat gtttatcata atgatatgac gatctgagtt tttaaaatca agaagaaaag
      601 ataagttttt taaatgcaca gtgcctgtgg ataactttgc gtaaatccgg aggacagatc
      661 actctgaagc aagacaacag catctgaaaa caatatgctc acgtttcaac cacagggcgt
      721 aagtgatcct acccacgtaa tatggacaca ggcctaagcg aggttctggt tttcaaattg
      781 ttccggactg aggccgccac accaactgtg ccgtcgccag cgattgtaat cacattcgat
      841 ataattaaac actgttgccc gcattatttc ccggctgata aagtgttctc cgtggagaca
      901 ttccactttc agcgaatgaa agaagctttc cacgcaggca ttatcgtagc agcagccctt
      961 tgcgctcata ctgccacgca gattatgcca cttcagcagc gcctgataat ctgctgaaca
     1021 gtactgactt cctcggtccg agtgaacgat aacgttccgg ggtctcttac gccgccacag
     1081 cgccatctgc agggcatcgc aggccagttg cgccgtcagg cgtggtgaca tggaccagcc
     1141 aataacggca cgtgaccaca ggtcaatgac cacagccaga tacagccagc cttcatctgt
     1201 acgtaagtac gtgatgtctc ccgcccactt ctggttcgga ccactggcgt aaaaatcctg
     1261 ctccagcaga ttttctgaca caggcaggcc atgtgcgcgg taactgaccg tattgctggg
     1321 taagatcaat ttgctcactg gtatttacta cgtgcatcga atgagtgatc aagagccgta
     1381 tgacgcgaga gtgtcacgta cggttccgta agcagccgga gagtgaaact ctctccggct
     1441 gactcgactc gcaggttccg acagcggtga cgaacatgca gcggtgttgt tctcgctgat
     1501 cggcacatgc cgtctgaaca atgtggagcc agaaaaatgg ctgcgttacg ttattgaaca
     1561 tatccaggac tgaccggcaa accgggtacg cgatctgttg ccttggaaag ttgatctgac
     1621 ttctcagtaa atatcaatat gattctgacg agccgcttac ctttgagcag agaaagggga
     1681 gagggatccc acatccatga gctaggaaaa tacaaaaact ggctatacaa caattcaata
     1741 tattaggact tacttgcgag gagaaatata gcaccgctca ggcaattcct gggtatttac
     1801 ttttcttagt ttccttactc gggatgttct ttcatcagaa aggcgattct aaatttgtaa
     1861 tatagtcact catattccca cgtaatttct ttagatcaat atattgcaca tttttctcaa
     1921 aaaactgatc ggaaataaga tttgcaacat tgcttgttgt atcattttta acgcttctat
     1981 tcatcccttc acaaagttcg tccattttct ttttgaaata actgcttcct gatacaagat
     2041 gatttatgga actttgtgca gcattgatgt ctagagaaat gcgataatct ttctcaacct
     2101 gtttaaatat ctgtttacgg agattatcgc cgatttttcg gctgtgttct gcacaaaact
     2161 tatcaataca ttggttcacc acccgggata cgtagacttt tttttcatca tttttccaat
     2221 ctgtaatatt ttcaccgatt tgttctgaaa cagagctcaa aatactagta tttattttgg
     2281 ttgatgatat ttttactatc cttttcccca ttgtacagcg gataatctca tccacctttc
     2341 ctgaaaaata tcttttgtga gataaactga ttccaaaatt attaaaagat gttccaagca
     2401 ttataatcac tttgtcatgt aaaatagtaa cattgtcatt attttagctg attcaaacaa
     2461 ttctgtaaat gctttgagtt ctttactccc ttggctcaat gatggaccgt ctggaatccc
     2521 agagactaaa ctctctgaga aagagagtat gaaaaaactt ggggcagtcc ctaaatgaag
     2581 ggaccaacag cggtaaaata accggcatca ttcataatga tgccctgtta gttgtagtta
     2641 tgaagattta cgtcattaaa attgctgttc gtagtgtcag tcctatggtc tggcatcagc
     2701 tgaggatcgt agccgataca tcactggctt cacgacattc gtgttgaggc tattcatgaa
     2761 aactccacgc tgaaaacgcc gttttgtata aacggtcacg gtatgcccgg agccactgtt
     2821 gctgatgaat tcgataaaac cttggcgttt cttgaagcta tcgttaatgc ggataatgaa
     2881 acaacgattg gtgagattcg ctcttttgcc gacgccctgg acgctgtccg gttcaatcgc
     2941 aacaaaatca accggcagct gagcaaaccc aaccttgcct ctctgccgct ggagcatgaa
     3001 gtgatttggc tgggacgtag ccgctgacag cttctataaa agtatgatcc tacccacgta
     3061 atatgaacac aggcttaagt gaggttctgg ttttcaaatt gttcaggact gagacctcca
     3121 caccaactgt gccgtcgtca gcgattgtaa tcgcacttga tataattaaa cactgtatat
     3181 ttcatttgtg gagtctccag tctgaggggg ctttatgcat tccgtattgg gcactatgat
     3241 gccagaaatc tgcaaggctc cacaccttcc tgtatttccc ttcctctatc acagtgcact
     3301 ttacttaata gggtgggatg tgttcattac atttattgtg ttttttatga ttgtttttgc
     3361 gtccagtgtc gttcgtcatg ggaaactggt tctatgggta gtatcgctca gtctagttaa
     3421 ctgtttgttt ctttttagaa attaagcaga tcacacaatt cggggagaga tccttctaag
     3481 tgcaattctt tgcagctatt tagcacactt aaaacaactc ttgtattttg tcagaaataa
     3541 tattaaacat gtcttgttca ttattgttaa gtctgtctgc tatttctgta agttttatcc
     3601 cactatgatg gcatatattt atatatgcca tcatagcctc gatattccct ttccctgcgg
     3661 ccagaaataa tcctggtaac ccctcattat ctcttacatc caatagttta atcgtttctt
     3721 ttggtgataa atcaaaagtt ttaataaaat ctccataagc ttgaatcgca gcggaatgac
     3781 cttcttgaag agctatgaac aaagcagatt ccccagcatt atttcgtgct gcaagaactt
     3841 ctagtttttc ctctttgggt atttcggctg ccaacagcga ttcaccgtat ttacgcgctg
     3901 catcggcatt tccatgccaa atcaatgaaa acaaattatg gctgttatac tccttattat
     3961 tctttgtcgg aatggatatg ctctgtattt tggccaattt tctgaaagaa acaccactat
     4021 atcccccccc tgatttttct tcattgatgg cgtgggatat agcggtattt gtcacgttaa
     4081 tgatattatt tttcattcct ttattttcat tagcagtatt gctgcctatt attaggtgca
     4141 tatttttgtt gtttgctgga tgtaatccat agttatttat tgacattttt gttcatcctt
     4201 tttgctaaaa aaaacaataa cctatttttg taatgtaata gatattagtc tattgctaat
     4261 ctaaaaaaac cattgccaca ttgaatggct atattcacca ataaattctt tcgatttcgt
     4321 atattgacat atcgtagata tatcttatcc ctaagattca ataatcgagt ccgtttacca
     4381 ttctgtgcaa gcgccttcac tcagaagtga ccgtcaaggc gatcaccaaa cttgataata
     4441 aagcagctca ttgccatacg ccagtcctta aggggcgttg tccatttctg tgatgctgcc
     4501 tagatttaac tgccagctcc ggcttatcta catctaaata tgtgagccta ataccgctac
     4561 actttgtcca gactatgttt ttctccggga agtgggctaa ccgtctccat aaagtgacga
     4621 acttttttca acagtggcca tattgaacgg cactgatgat tacgggttat tgtctcatga
     4681 agagccggcc acatcactga cagcagtgac ttcaggcctt cagcaccaga cagcctgaac
     4741 cagttaatcc atcgcccgac agatttagta atctagggct ggtttttctt cagctgttgc
     4801 ggttcgaaag tgccattacg atcacgcaga gtacggattt ccagaggttc atctccagta
     4861 gtgataatct tcgttaagca accattgcgg gaattgctgc cagatctaag ctgatttttc
     4921 tcgcacccaa gatgatgagt catattagca ttgagtacgg tttcattttc agcatccaat
     4981 caaactgact gagatcttca ggatttttga gacttttggc tagttcatta gctaaagccc
     5041 gcaacttatt agatgtcaac tgtttttcat tcataaattc accttcattt gatatcaaat
     5101 taaatagttc aacatcagag aattatacaa acttatgtac aggctcctat gatcttcgaa
     5161 tcctgaagat tatagtgaaa ataagttttc acgttgaaat cccacatagt tagtatatga
     5221 taaataatca tggatgacct gattaacctc ttggggtagt gcttttatag aagaataaat
     5281 attctactaa tttaatacat cacctaaaat agtattgttt tttatggttg gttgaaggtc
     5341 gtggtgatga tgttagtcct tttgagaata ttccagtgcc agatcttact tataataaag
     5401 gggcttggca gtttggataa actattgttc ttattccact aatacaaact ttttgtaaag
     5461 tatattaaat atacttcatt tcatactgaa atccccggaa gtattcaatc aaacaattac
     5521 accataaaag gaagaggggg ccctaattaa gttgtcaagc atgtttatga tacctgcagg
     5581 gtataaattt tctggctgca agcagacaca tataaaacgt ctttattgat cctacccaca
     5641 taatatggac acagtcgtaa gtgaggctct ggttttcaaa ttgttccgga ctgagaccac
     5701 cacaccaacg gtgccgtcgc cagcgattgt aatcgcactc gatataacta aacactgttg
     5761 cccgcactat ttcccgatgg ataatagtgt tctccgtgga gacattccat tttcagcgaa
     5821 tgaattttca tctgttgctg tatccagtca tacagtgact gcatcagtgg cgcggctctg
     5881 gcttttcttg ccgccagacg ctgttctgct gaacatcccc ggacttctgc ctcgatggca
     5941 tacagttcac cgatacgctg cagggcttcc gtggtgatgt cggttggcac tcttgcatgc
     6001 acatcgtgga tttttctccg gacatgagcc atacatgcgg cttccgttat tctgccggat
     6061 tcgtacaacg cccggtaacc accgtaagca tcggcctgaa gcactccgct gtaaccggcc
     6121 agatgattct gtggatggat acctttccgg tccggtgagt acgcgaacca gaccgccgga
     6181 ggcatttccg aaccggcgtt acggtcatca cgaacgtaga cccacagccg ggcagtcctt
     6241 gttttaccgc tgcccggctc ccggaccgga acggggatat catcggcatg gactttaccg
     6301 ggcatcagca catactggcg caggatgtca tacagcggtt ccagcagttc agcaacagca
     6361 cctgtccagc gccccagtgt ggcacggctc agctccacgc cctgacgacg gtatatttct
     6421 gactggcggt ataacggcag atagtctgca tattttccag cgacaacatg ggccagaagc
     6481 cccgctccgg cataactgcg tgcaatgggt tttgaaggca ccggtgcctg cacgatatgg
     6541 tcacaccggc aacaggccag tttcggacgt tgtgtttcga taaccttaaa ggcgctgctg
     6601 ataagctcca gctgctctga cacatcacat cccagagaac tgagttcgcc accacaggcc
     6661 gggcagcatt cctcttccgg acggatgacc cgggtttcac ggggaagtga ggcaggtaac
     6721 ggtttacggg ctgaagactg gcgcagggga gatggcagta ccgggtcata ttgctcaccc
     6781 agcgtttccg ccatttcttc ctgaagtgcg ctgattcgct cctgtgcttc cagtatctgc
     6841 cgttcggttt ttgcgcgaag tttttctgag cttttaccga actgcatgcg ctgcagtttc
     6901 gcaaccagcg ccttcagccg gttgatttcg gaagcataag ccgccacccg ctgtgagagc
     6961 aggcggttgt atttcgccat ctggcggatg gtgtcctgtt gcgtctgcaa cagtgcccgc
     7021 aggcgggcat tctcatgaga aagtgaggtg tccatatcct cactttacaa cgggttatat
     7081 gcggatgcca gcgcgttccg ttcgtttcgg gtgtttccag tcgatgccct caagaagcat
     7141 ggataactgg gccggagtaa ggtgcacctt gccgtcacgg gtgaccggcc agacgaagcg
     7201 gccccgctcc aggcgtctgg taaagaggca cagtccgtca ctgtcagccc acaacacttt
     7261 tatctggtca ccccggcgtc cgcggaagat gaacagatgc ccggagaacg ggtcatcctt
     7321 caggacgttc tgaacttttg aggccaggcc gttaaagcca tttcgcatat cggtgatacc
     7381 tgcaaccagc cagatacgcg aacctgcagg gaaagatatc atcagtggct gctccctttt
     7441 atttcgcgga taagtatctg taataacgcc ggcgtcagtt tacctttaag cctgagagtt
     7501 ccggccggca gaaccagctc acagcacaga ctgtcggacg gtgtatttat ctgctccggc
     7561 gttaacgtca cgggaagcag tgcaggcata ttttttccgg aaggcagcag gccacctttc
     7621 cggtattgat ggcgccagtt gaagagcagg ttatcgttga ttccgttttc acgggcgatc
     7681 tgcgccacac aggctccggg ctgcagtgac tgctccacta aagcgatttt aaattcataa
     7741 ggaaaattag gcc
//
LOCUS       DADMEQ010000154         7739 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.156, whole genome shotgun sequence.
ACCESSION   DADMEQ010000154 DADMEQ010000000
VERSION     DADMEQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7739
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.156"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            163..648
                     /locus_tag="JNZ05_003589"
     CDS             163..648
                     /locus_tag="JNZ05_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566660.1"
                     /translation="MRAIYLSVQQAWNGKITYSVSGESEFAKKFQGKALPFDVRIISA
                     SQNEDWLVIATKVLPGADLRTYVDFKNSTVHVDSADLEKVAKCINCNNTLQVNIPHEA
                     GHVLGYLDDDYDSSSPYVGDISGLMNVGMELRERYLKNATITLNVIMPETKFTLLNVT
                     K"
     gene            645..1031
                     /locus_tag="JNZ05_003590"
     CDS             645..1031
                     /locus_tag="JNZ05_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566661.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGDYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LACWFHYKDGQWRVITYIFWPPGARF"
     gene            1056..3268
                     /gene="tssI"
                     /locus_tag="JNZ05_003591"
                     /pseudo
     CDS             1056..3268
                     /gene="tssI"
                     /locus_tag="JNZ05_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
     gene            3291..3735
                     /locus_tag="JNZ05_003592"
                     /pseudo
     CDS             3291..3735
                     /locus_tag="JNZ05_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3758..4165
                     /locus_tag="JNZ05_003593"
     CDS             3758..4165
                     /locus_tag="JNZ05_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HAY8566662.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4162..6640
                     /locus_tag="JNZ05_003594"
                     /pseudo
     CDS             4162..6640
                     /locus_tag="JNZ05_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            6637..7495
                     /locus_tag="JNZ05_003595"
                     /pseudo
     CDS             6637..7495
                     /locus_tag="JNZ05_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706221.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
BASE COUNT         1971 a         1807 c         2260 g         1701 t
ORIGIN      
        1 acaaatattg atggcaatat cattatacat aaggatggta ggaggacgta tttattaaat
       61 caaaatactg gttcgattac ggctgtgata agatggaagt atgagtagta taaggataat
      121 accgttacag atgactggac tatatgggaa aaagctattt ttatgcgagc gatatattta
      181 tctgttcagc aggcatggaa tgggaaaata acttattctg tttccggtga atctgagttc
      241 gctaaaaaat ttcagggtaa agctttgcct tttgatgtaa gaataatttc tgcatctcag
      301 aatgaagact ggttggttat tgcaacaaag gttttgcctg gtgctgattt aagaacatat
      361 gttgatttta aaaacagtac ggttcatgtt gattcagcgg acctggaaaa agtagcgaaa
      421 tgtattaact gtaataatac gttgcaagta aatatacctc atgaagccgg acatgtactt
      481 ggttacttag atgatgatta tgattcttcg tctccgtatg ttggagatat tagtgggttg
      541 atgaatgtgg gaatggagct tcgggaacga taccttaaaa acgcaactat aacgttgaat
      601 gtcataatgc ctgaaactaa atttacttta ttgaatgtaa ccaaatgaaa tttttctgta
      661 ttatagtgat tttcctttca ataaattaca tagctatagt gaatgttttc aagtcttctg
      721 cagttgctca atccaccaaa tattatgaag aaaatgatat acatgatgga agtaatgaca
      781 tcttttttat gaagaaatat gggccgcctg ctcatgagtt cgtgaggcca cgatataaat
      841 ttgttggtga ttattatatc ggcttgaatc aaacttatcg cataacagat cctgaatttc
      901 aagatgttcc tattaaggaa atgttctgga atttgcaggg ggatttgaat ctggcatgtt
      961 ggtttcatta taaagacggg caatggagag tgattactta tattttttgg ccaccagggg
     1021 ctagatttta atccggtatt aaaggagcaa atatcatgag ttttgtatct acgggtaata
     1081 aaccagttgg aaacggtgga cccgtcatca ccacaccacc aatagctggc gaaagtggcg
     1141 gtatgagcac aggttcagct gtaaccgatg tatctggtgc cgccgaggag atggcagaac
     1201 aggcagcggc ggacctgttt ggtgcgctgc cggaaccgtc aggtctggtt aaagccgcag
     1261 tagctgctgc gcaggctgcc gctgcagcag cagggatctc ggatatggct ggtgcagttc
     1321 aggatgcggc ggcgtcgctt gcagccggtg gtccgggggc gcataacgtc acagtaagtg
     1381 gtagtgcggt gccgccgcag atgcttttgt ttgcaggcat gaatggttcg gaagggcttg
     1441 ggaatctgtt ttcttacaca gtacagctta aaacgctgga cgccctgaac ctggggtacg
     1501 tttcccccgc tgctaacctg ccgcttcagc tcatggtggg caaagacctg tgcgtcagta
     1561 tcgaactgga cggcggcgga aaacggtata tcagcgggct ggtgacggcg gcgagggtgg
     1621 tcggccatga gggccgttcg gtaacgtatg aattacgcat tgagccatgg ctgaagctgc
     1681 tgacccatac cagcgactac aaagcgttcc agaacaaaac cgtggtggat attctcgatg
     1741 aagtactgga tgaatattcc tttccggtgg aaaagcggct ggtggaaaat tatccacccc
     1801 gcgcctggca ggtgcagtat ggtgaaaccg acttcgattt catccagcgc ctgatgcagg
     1861 aatgggggat ttactggtgg tttgagcaca gcgagaacag ccacacgctg gtgctggtgg
     1921 atgccatcaa tggtcataaa gcgtgccctg actcgccgct ggtggagtgg caccaggaag
     1981 ggctgaaact ggataaggaa tttatccaca ccattacggc cagtgagagg ctgcgtactg
     2041 gaaagtgggt gatggacgat tttgatttta tgaagcctcg ttcgctgctt aaaagtaccg
     2101 tggccagtcc gcgtgatacc ggtcatgctg aatacgagca ttacgaatgg cctggtgatt
     2161 acttcactac gggcgaaggc gagatgctga cgcgcattcg tatggaagcg cagcgcagcc
     2221 caggcagccg gcacatgggg ccggtcatat ccgtaccctc atgaccgggt acacctttac
     2281 gttgatgaat cacccgacgg cagaaattaa tcaggaatat ctgctggtac agaccactct
     2341 gtttttacgg gataatgcgc agcacagcgg tcaggatcag cattttacct atgtcaccac
     2401 gtttgaactg ccccccacca gcgaagttta ccgcccacaa cgaaccctga gcaaacccca
     2461 caccaaaggc ccgcagagcg ccatcgtcac cggcccggcg gggcaggaaa tctggacgga
     2521 taaatatggc cgggtgaagg tgcagtttgg ctgggaccgt tacggaaaga atgacgaaaa
     2581 cagttcctgc tggatccgcg tcagctatcc gtgggcgggt aaaggattcg gcatgatcca
     2641 gatcccgcgt attggccagg aagtactggt ggatttcaaa aacggcgatc cggatttgcc
     2701 catcatcgtg gggcgcacct ataaccagga caccatgccg ccgtgggggc tgccgggcat
     2761 ggcgtcgcag agtgggatat tcagccactc attgcaggga gggccgacta acggaaatat
     2821 gctgcgcttc gatgataaaa cgggtgcgga agaggtgaag ttccatgcgg aaaaagacct
     2881 caataccacg gtaaaaaaca atgaaaccca cacggtcatg gtcgatcgca taaaaaccat
     2941 tgtcaaaaat gaaaccatca gcgtgggaga agaccgtaat gccaccatca caaaaaatga
     3001 tggtctttcc gtcaaactgg ctcaaaccat caatgtcgga acgacatatc gtctggatgt
     3061 tggcgatcaa tttacccttc gttgcggcaa tgcagcgctt gtattacaca aggatggctc
     3121 gattgagttt tgcggaaaac aactgttact gcacaccagc gatgttatgc aactcattgg
     3181 cagtggcatt gatatgaatc cggatggcgg cacagccatg actgcggatg atattgcccc
     3241 gttacctacc cccgacgttt ctgaataaaa aatataaaag gaatttttct atgaccagcc
     3301 tttaccgtat tcaggagggc tgctttgccc tgcctgaaac ttttcttgat cgcaccgtca
     3361 atatcttcgt cccatcgggc aatgaaaggg caactcccag tctgaatatt tcccgcgata
     3421 cgctcaggcc agatgaaagt ctgaccacct atattgaccg ccagatagcc ttgatgaaaa
     3481 aaaaacctgg gccagcaccg ggtactttcg cggggaacgg cactggcggg aactggcgaa
     3541 gctgccatga caggtgaaca gattctcgca actcatcgtt ctggaaaaac agaagtgtac
     3601 cagcgtcagg ccgggtttat caccgggcca gcaaaggtac taatgttgac gctgaccact
     3661 cagcgtcctt ttgatgatca caccgaccag ctatggactg catggttgac tagttttcag
     3721 cctgctaaaa gctaaaaaaa aacagggagg ttttgtcatg tatgaagccg cacgggtgga
     3781 tgaccccatc taccacacca gcgcccttgc cgggtttttg ataggggcca tcataggtat
     3841 cgccattatt gccgtcgcca gctttgcttt ttttacctgc ggctttttgg ccgggctgat
     3901 tttgagtttt ctggctgatc agatagcttc cggagtcctg caactgggtg aggctattgg
     3961 gcgctcgata cattccacag tgggaaaaat tctgaccggt tctgataatg tcagcaccaa
     4021 cagtcgcccg gccgcccggg ccgtactgag tacggtgata tgcgaagacc attccccgga
     4081 aatccgcatt gcccagggtt ccggcaacat ctacatcaac agccagctgc ccgtaaggat
     4141 gaccacactg agtgtgatgc agtgattgag gacggctccc ccaatgtctt tctcggcggc
     4201 ggtacgcaga cggtactgga aatcagcccg gaaatccctg actggctgcg tcaggtggtg
     4261 gatgtgctgt atgtggtcgc cggaatgctt ggcgggctgg ctggcgcctg gcgtcaggcg
     4321 gcaaagatgg ggtcgaaatt tggtacaaaa tgtgcggcaa aatttattgg tggcgaaatg
     4381 gtcgggatgg ccatctctga cacagtcatg ggattgttca gcaatccggt agatgtcacc
     4441 actgggcaaa aaattttgct gccggagacg gattttaccc tgccaggacg cctgcctgtc
     4501 acctgttcgc gcttttacgc cagccacctg gaaactgaag gattgctggg cagaggatgg
     4561 cggctgaact gggagataaa cctgcgtgag gatgaaacat atatcacctt catcggtgta
     4621 cagggtcggg aactgagtta tccgaaagag atgctgatac cgggccacca gatattcgac
     4681 ccggaagagc agttttacct gagccgtctg catgacggat gctatgtgct gcattacacc
     4741 gaccgcagct attacgtgtt tgatgaattt gatgaccacg gcgtcgcgcc gttacttttt
     4801 atggagacgc cgtaccggca gcgtattgct ttcgggcgtg agaacggcag gctggtgcgg
     4861 gtggcctcca gcagcggaca ccatctgtta ttgcaccgca cgatgacgca agcaggggaa
     4921 cgcctgtcgc atattgaact tgtgaagggc gggcgtcccg gtaacctggt ggaatatcgt
     4981 tacgacgata acgggcaact gactggcgtg gtgaaccggg ctggtgtgac ggtgcgccag
     5041 tttgcctatg aaaatggcct gatgacagag catcgcaacg cgacgggctt tacctgtacc
     5101 taccactggg aagagattga aggtttcccg cgcgtggtgg aacacacgac cagcgacggc
     5161 gaggattacc ggttccacta tgattttgct ggcgggcaga cggtggtaac cggcaggccg
     5221 gagcagaagt ggcagtggtg gtttgatgaa gagacgtatg tgactgcgca ccggacgccg
     5281 ggcggcggaa tgtaccgctt cacgtacaac gaaaaccatt ttccggtagc ggtggaactc
     5341 cccggcgaac gcagggtaac gctggaatac gacaccctga gccgggtggt gaaagagacg
     5401 gacccggcag gccgggtgac acagacgcag tggaacggca gttttgctga aatcacgcgc
     5461 cgggcgctgg atgacgacca cgtatggaag gcggattaca acgaacacgg ccaggtgata
     5521 cgggagacgg acccggaagg gcgcgtcacg cggtacgggt atgatgaaca ggggctggcg
     5581 gtcagccgca cggatgcgcg gggcggcgag gttgtgctgg cgcacgatgc gcgcggtcaa
     5641 ctgaggcgtt acaccgactg ttcagggtgc gccacgggtt atgaatacga tgaagacggc
     5701 aacctgacgg cggtgacgga cgcggagggg aaaacggtac gcatcagcta taaccgtcag
     5761 gggctgccgg agacggtctg ccaccccggt aaacagcagg accgttacac ctggaacgcg
     5821 ctggggttac tgagcagcca ccgtcgcatc acgggcagtg tgcagtcctg gcagtatacg
     5881 ccgcgcggga tgctggcgcg ccatacggat gaagagaaac gggagacgcg ctggcagtac
     5941 accccggaag ggctggttgc cagtctgagc aacggcaacg gggctcagta ccggttcagc
     6001 tacgacggcg acggacggct gacgggagaa cagcgcccgg acggcctgat aaggatgttt
     6061 gcgctgaatg cggacgggtt tccggtcatc atccagacgc agggcacaga gggcggggtg
     6121 cgcaatgaac agcaggagcg tgatgcgctg ggccgcctgc tgcgcagtga cacgcagcac
     6181 agtacacgga ccttcagcta caaccgtctt gaccagataa cggaagtgac gctcaccccc
     6241 acggaagaag gggaacggct gcaccacatg caggcggaca cggtgcgttt tgcgtatgac
     6301 cgcagcgggt ggctgacggc agagcacagc gtgcacggca gtatcaaata ccggcgcgat
     6361 gcgctgggca atcccaccga tatcacgttg ccggacgggc agcatctcag tcatctttac
     6421 tacggcagcg ggcatctgct ccagacggca ctggacggta tcacggtcag tgaatatgag
     6481 cgtgacagcc tgcaccgtca ggtgatacgc acgcagggga aactggcgac gttcagcggc
     6541 tacaacgccg acaaccggtt aagctggcag cgcagcctgc caggtggcag ccagaatcca
     6601 cagcaggccg gtactccccc ggcgcagaac gacagcgtga cggcgcggga ctatatctgg
     6661 aacgcagacg gtgaagtcgg cggcatcaat gacaaactgc gcggttgcct ggtgttcagc
     6721 tatgaccgca gcggctggct gaccagccgc acggggcaga tgtatgacca tgaccattat
     6781 tattacgaca aagcgggcaa cctgctgacg gatgagtatc agggagcggt gatggataac
     6841 cgcctgccgg gctacgggcg tgaccgttac cgctataacg aatggggcga actgacagag
     6901 cgcagggacc agcaactgga gtggaacgcg cagggccagt taacgcgggt catcagcagc
     6961 aacagcgata cgctgtatca gtatgatgcg ctgggcaggc gcatcagcaa ggtgacatat
     7021 ggccgccatc acggggcgcg gagagagcca gaaaatctgg tattaccaca cggacctgac
     7081 gggcacggta cacgaggtaa ccgcgccgga cgggacgctg gtgtgggcgg gctaccaggc
     7141 gggatttggt gaaaaccggg gcgacatcag caacagcggg gcgtactttg agcagccgca
     7201 gcggctgccg gggcagtact ttgacgaaga aacagggctg cattacaatc tgttcaggta
     7261 ttatgcgccg gagtgcggga ggttcgtaag ccaggacccg ataggattaa acggtgggtt
     7321 gaacttatat gcttatgcgc ctaatccgtt aggttggatc gatccgctag gactgaattc
     7381 tttaggaata gattcaaata atatatttag aggagatggt aactataaag gtggcggaat
     7441 tggacagcca aagagtggca caatatcagc acaggacatt attgatcatc tctaagaaaa
     7501 aggtgatggg cgtttaacat cttttagtac taaaaaaaca tctagttcca actctcgtgg
     7561 tgcagatttt ttctcaaaaa accatgtctt caaagtggca gtttctgatc tcgatgattt
     7621 agttaaaaat aatcagatca ctgttttaac acaagaaaaa gtaaaagcaa tatttggttt
     7681 agaatcaagc aagaaaatta gaaaagaact tggtaatatt cttcagaata tggctagga
//
LOCUS       DADMEQ010000155         7561 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.157, whole genome shotgun sequence.
ACCESSION   DADMEQ010000155 DADMEQ010000000
VERSION     DADMEQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.157"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..1675
                     /gene="cpdB"
                     /locus_tag="JNZ05_003596"
     CDS             <1..1675
                     /gene="cpdB"
                     /locus_tag="JNZ05_003596"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HAY8566663.1"
                     /translation="GLKAGDVHPVYKALNTLDYTVGTLGNHEFNYGLDYLKNALAGAK
                     FPYVNANVIDARTKQPMFTPYLIKDTEVVDKDGKKQTLKIGYIGVVPPQIMGWDKANL
                     SGKVTMNDITETVRKYVPEMREKGADVIVVLAHSGLSADPYKVMAENSVYYLSEIPGV
                     NAIMFGHAHAVFPGKDFADIEGADITKGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGK
                     WQVTQAKAEARPIYDIANKKSLAAEDSKLVETLKADHDATRQFVSKPIGKSADNMYSY
                     LALVQDDPTVQVVNNAQKAYVEHYIQGDPDLAKLPVLSAAAPFKVGGRKNDPASYVEV
                     EKGQLTFRNAADLYLYPNTLIVVKASGKEVKEWLECSAGQFNQIAPNSTKPQSLINWD
                     GFRTYNFDVIDGVNYQIDVTQPARYDGECQMINVNAERIKNLTFNGKPIDPNAMFLVA
                     TNNYRAYGGKFAGTGDSHIAFASPDENRSVLAAWIADESKRAGEIHPAADNNWRLAPI
                     AADKKLDIRFETSPSDKAAAFIKEKGQYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(1801..2181)
                     /locus_tag="JNZ05_003597"
     CDS             complement(1801..2181)
                     /locus_tag="JNZ05_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY8566664.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            2270..3130
                     /gene="qorB"
                     /locus_tag="JNZ05_003598"
     CDS             2270..3130
                     /gene="qorB"
                     /locus_tag="JNZ05_003598"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY8566665.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAALVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIARPTTTLAE
                     SVSHLFNVNN"
     gene            3239..4204
                     /locus_tag="JNZ05_003599"
     CDS             3239..4204
                     /locus_tag="JNZ05_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY8566666.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLSQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            4312..4974
                     /gene="ytfE"
                     /locus_tag="JNZ05_003600"
     CDS             4312..4974
                     /gene="ytfE"
                     /locus_tag="JNZ05_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HAY8566667.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(5019..6431)
                     /gene="cycA"
                     /locus_tag="JNZ05_003601"
                     /gene_synonym="dagA"
     CDS             complement(5019..6431)
                     /gene="cycA"
                     /locus_tag="JNZ05_003601"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HAY8566668.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDFSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(6739..7359)
                     /locus_tag="JNZ05_003602"
     CDS             complement(6739..7359)
                     /locus_tag="JNZ05_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY8566669.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
BASE COUNT         1942 a         1973 c         1928 g         1718 t
ORIGIN      
        1 aggattaaaa gcaggtgatg ttcatccggt ttataaggcg ctgaatacgc tggattatac
       61 ggtcggtaca ctcggcaatc atgaatttaa ctacggtctg gattacctga aaaatgcgct
      121 ggcgggagcg aaattccctt atgtaaatgc caacgtcatt gacgccagaa ccaaacagcc
      181 aatgtttaca ccgtatttaa ttaaagatac cgaagtggtc gataaagacg gaaaaaaaca
      241 gacgctgaag attggctata ttggcgtcgt gccgccgcaa atcatgggct gggataaagc
      301 taatttatcc ggaaaagtga cgatgaatga tattaccgaa accgtgcgca aatacgtgcc
      361 tgaaatgcgc gagaaaggtg ccgatgtcat tgtcgttctg gcgcattccg ggctgtctgc
      421 cgatccgtat aaagtaatgg cggaaaactc agtttattac ctcagtgaaa ttccgggcgt
      481 taacgccatt atgtttggcc atgctcacgc cgttttcccg ggtaaagatt ttgctgatat
      541 cgaaggggct gatatcacca aaggcacgct gaatggtgtt ccggcggtaa tgccgggcat
      601 gtggggcgat catcttgggg tggtcgactt acaactcagt aatgacagcg gtaaatggca
      661 ggtgacgcag gcgaaagcgg aagcacggcc gatttacgac atcgccaata aaaaatccct
      721 cgcggcggaa gacagcaagc tggtagaaac actcaaagcc gatcacgatg ccacacgcca
      781 gttcgtcagc aagccaatcg gtaaatctgc cgacaatatg tatagctatc tggcgctggt
      841 gcaggacgat ccgaccgtgc aagtcgtgaa caacgcgcaa aaagcgtatg tcgaacatta
      901 cattcagggc gatccggatc tggcaaaact gccggtgctt tcagctgccg caccgtttaa
      961 agttggtggt cgcaaaaatg acccggcaag ctatgtggag gtggaaaaag gccagttgac
     1021 cttccgtaat gccgccgatc tttatctcta ccccaatacg ctgattgtag tgaaagccag
     1081 cggtaaagag gtgaaagagt ggctggagtg ctccgccgga cagtttaacc agattgctcc
     1141 taacagcacg aaaccacagt cactcatcaa ctgggatggt ttccgcactt ataactttga
     1201 tgttattgat ggtgtgaatt atcagattga tgttacccaa cccgcccgct atgacggcga
     1261 gtgccagatg attaatgtca atgcagagcg gattaagaac ctgaccttta atggcaagcc
     1321 gattgatccg aacgctatgt tcctggttgc caccaataac tatcgcgctt acggcggcaa
     1381 atttgccggt acgggcgaca gccatatcgc ttttgcttca ccggatgaga accgctcggt
     1441 gctggcagcg tggattgctg atgagtcgaa acgtgcgggg gaaattcatc cggcggcaga
     1501 taacaactgg cgtttagcac cgatagctgc cgataagaaa ctggatatcc gtttcgaaac
     1561 ttccccgtca gataaagccg cagcgtttat taaagagaaa gggcagtatc cgatgaataa
     1621 agtcgcgacc gatgatatcg ggtttgcgat ttatcaggtg gatttgagta agtaaaacac
     1681 ttctttttcg gcctataaat caaccgcatc cggcatttat tggcggatgc gatgctgccg
     1741 tatcttatcc accctacaag ccatgaaccg tagaccggat aagcgcagcg catccggcag
     1801 ttatgccgca cgttcatccc gcaccgccaa cacctcgggc aaattcaact caatccagtc
     1861 cgccagtgca gcaacctttt cgctcacctg ctcgcccagc ggcgtgaggc tatattccac
     1921 atgcggcggc accaccggat acgcgatacg gttaagaaaa ccatcctgtt ctaacgcctg
     1981 taacgactgc gcaagcatct tttcactcac cccaccgatt ttgcgccgca ggtcgctaaa
     2041 gcgatgagta ccttcgcgta gcgccaccag aatcaacacc ccccaacggc tggtgacgtg
     2101 tttcaacacc tcgcgcgacg ggcactgttc cgcaaagaga ttaccctctt tcagttgttg
     2161 tgacaggcta acctgactca tttcatactt acctttttgc atgtacttac taaaagtaag
     2221 tttaggtgtt agcgtattta aacacaagac aaaacgatgg agacttccca tgatcgctat
     2281 tactggtgcc actggccaac ttggtcacta tattattgaa tccttgatga aaacggttcc
     2341 tgccagccaa atagtggcta tcgttcgtaa tccggcaaaa gcccaggccc tggcagcaca
     2401 aggcattacc gtgcgtcagg ctgactacag cgatgaagcc gcactgacat ctgcacttca
     2461 gggagtggaa aaactactgc tgatctcttc cagcgaagtg ggtcaacgtg ccccgcagca
     2521 tcgtaatgtt attaatgccg caaaggcggc tggcgtgaaa tttatcgctt ataccagcct
     2581 gctacatgca gatacctccc cgctcggcct cgccgatgag cacatcgaga cggagaaaat
     2641 gctggctgat tctggcatcg tttacaccct gctgcgcaac ggctggtaca gcgaaaacta
     2701 cctcgccagc gccccggcag cactggaaca cggcgtattt atcggtgcgg cgggcgatgg
     2761 caaaatcgcc tcagcaacgc gggcagatta tgcgacagct gcggcactcg tgattagcga
     2821 agccggtcac gaaggcaagg tttacgaact ggcgggcgat agtgcctgga cgttgacaca
     2881 gttagcggca gagctgacca aacagagtgg caaacaggtt acctatcaaa atctgagcga
     2941 agccgatttc gccgcggcac tgaaaagcgt cggactgccc gacggactgg cggatatgct
     3001 ggcggattct gacgttggcg catcgaaagg tggtctgttt gataacagca aaacgcttag
     3061 caaattgatt gcccgcccaa cgacaacgtt agctgaaagc gtaagccatc tttttaatgt
     3121 taataactag ttaattaaag tggcatcctc ccgcatcctc tctgataatg acgggatgcc
     3181 gggagcaatc atgtctgctt cctgaacgtt tcttctgaca gaccaatgga tgccagtaat
     3241 gattagcggc gtgctgtacg ccctgttagc agggttgatg tgggggctta tttttgtcgg
     3301 gccgttgatc gtgccggaat acccggcgat gttacagtcg atggggcgtt atctggcgtt
     3361 agggttaatt gcgctaccca ttgcctggct gggacgcgtg cgtctgcgtc agttggcgcg
     3421 tcgggactgg cttaccgcct tgatgctcac tatgatgggc aacctcattt attacttctg
     3481 ccttgccagt gccattcaac gtactggcgc gcctgtttcc acgatgatta tcggcaccct
     3541 gccggtggtg attcctgtct ttgccaatct gctttatagc cagcgcgacg gcaaactcgc
     3601 gtggggaaaa ctcgccccgg cactgatttg tattggcatc ggcctggcga gtgtgaatat
     3661 tgctgagtta aaccacggac tccccgattt tgactgggca cgttatacct ctggcatcgt
     3721 gctagcgtta gtttccgtgg tctgctgggc atggtatgcc ctgcgcaacg cccgctggct
     3781 gcgggaaaat cccgacaaac atccgatgat gtgggcgacg gcgcaggcgc tggtcacgct
     3841 gccggtttct ctcatcggct atctcgtcgc ctgttactgg ctgaatatac aaacgccgga
     3901 cttctcctta ccttttggcc cccgtccgct ggtgtttatt agtctgatgg ttgcgatagc
     3961 cgtgctttgc tcatgggttg gcgcactctg ctggaacgtc gccagccagc gattaccgac
     4021 agtgattctc gggccgctga ttgttttcga aacgctggca ggtttgctgt acaccttttt
     4081 actcagccag caaatgccgc cgctaatgac gctgagcggt atcgcgctgt tagtgattgg
     4141 cgtggtcatc gcggtcagag caaaaccaga aaaaccttta actgaatctg tctcagaaag
     4201 ttgacatgct ggcagtgagt taaataagcc tctgctacgt aagggttata gcttttgcct
     4261 taaaggtgta tttaaaatac atcttatctt attaagaatg aggtatcagc tatggcttat
     4321 cgcgaccaac ctttaggtga actggcgctc tctatccctc gcgcttcggc tctgtttcgt
     4381 aaatatgata tggattactg ctgtggcggt aagcagacgc tggcgcgcgc ggcggcacgt
     4441 aaagaactgg atgttgaggt cattgaagct gaactggcaa agcttgctga acaaccgatt
     4501 gagaaagact ggcgtagcgc cccgctggca gaaatcattg accatatcat cgtgcgctac
     4561 cacgatcgtc accgcgagca gctgccggag ctgatcctgc aagcgactaa agtcgagcgc
     4621 gttcacgccg acaaaccgag cgtgccaaaa gggctgacaa aatacctgac catgctgcat
     4681 gaagagcttt ccagccacat gatgaaagaa gagcagatcc tcttcccgat gatcaaacaa
     4741 ggcatgggca gccaggcaat ggggccaatc agcgtaatgg aaagcgagca cgatgaagcg
     4801 ggcgaactgc tggaagtgat caaacacact accaataacg tcacaccgcc gccagaagcc
     4861 tgcaccacct ggaaagcgat gtataacggc attaatgaac tgattgatga cctgatggat
     4921 cacatcagtc tggaaaacaa tgtactgttc ccacgcgccc tagcgggtga gtgatctaaa
     4981 agctggatgg cattgcgcca tccagcatga taatgcggtt atttccgcag ttcagcagcc
     5041 cgcttcttac caataaacag ccagcccaac cccagcgcga taaaccacag cggggtaacc
     5101 agcagcgcct ggcgagtgtc atcttccagt gtcagcaaca cgaccacgaa cacaaagaac
     5161 gccatacata cccagcacat cagcttgccg agcggcatct tgtagatcga cttttcatgc
     5221 agatgaggac gctgtttgcg gtacaccagg tacgagcaaa ggataatcgt ccagacgaac
     5281 ataaacagaa tcgcggaaac ggttgtaatc atcgtgaacg cgccaatcac gctaggattc
     5341 acatacaaca tcaccacgcc gccgagcaga cagatacacg agaaggtcaa ccctttcgcg
     5401 ggtactgcgc gcttagaaag tttagcgaac gctttcggcg caacaccttc ctgcgccaga
     5461 ccaaacagca tacggctggt agagaagaca ccgctgttag cggaagacgc cgcagaggtc
     5521 agcaccacaa agttgatcac gctggcagcc gcaggcaaac ctaccaacac aaacagctca
     5581 acgaacgggc ttttctccgg gactaccgaa ctccacggtg tcacggacat aatcacaatc
     5641 agcgcgaaga cgtagaacat aatgatacgg atcggaatgg agttaatcgc gcgtggcagt
     5701 gatttctccg gatctttggt ttccgcagct gttgtaccta ccagctcaat ccccacgaaa
     5761 gcgaaaaccg ctatctggaa tccggcaaag aagccactta aacctttcgg gaaccagccg
     5821 ccgtcattcc acaaatgcgc gaatgacgct tccacaccgg tcggtgactg aaagtgcatc
     5881 gccaccatga ccaggccgac gacaatcagc gacacgatgg cgacgatttt gatcatcgca
     5941 aaccagaact ccatctcacc gaacattttc acggtggcaa ggttgagcgt cagcagcagc
     6001 actatcaccg ccagcgaggc gacccagtcg gagaaatcgg ggaaccagaa ctgagcataa
     6061 gccgtgatcg ccaccacgtc tgccataccg gttacaaccc agcagaacca gtaagtccag
     6121 ccggtgaaat atcccgccca cggccccagt aaatcggaag cgaagtcact aaaagatttg
     6181 tattccagat tcgaaagcag caattccccc attgcccgca tcacgaaaaa gagcataaaa
     6241 ccaatgatca tataaacgaa aatgatcgac ggcccggcaa ggctaatcgt tttgccggac
     6301 cccataaaca acccagtacc aatggcaccg ccaatggcaa taagctgaat atgtcggttt
     6361 gtgagattgc gccgtagcga ctgttcagcc ggagcctgat catcggcaac gacttttacc
     6421 tgatctacca tgttttttct tcctgtacct gtctgtgttg ttcaggctct acggccttta
     6481 gtcagtctat gatacgacga atctgtatcg ggactcatcg atattaggta acaatacgcg
     6541 ggatgaatac taagatttag atctaatgtt aattttatgt ttaaagtgag tgtcatatca
     6601 cggtgataac gcgaaacagc tcacaaaaat acacgcttac ggggtctttg caacacaaaa
     6661 tagcgccgtt tggcaaactg aagggtttat tgctgaatgc ctgctcccct ctggttcgag
     6721 gggaacagaa tgcgctgctt agaggatttc cagcagttcg acttcaaaca ccagggtgct
     6781 gaacggaggg atggatgcac ctgcgccgcg ctcgccatat gccagttcct gcgggatagt
     6841 cagttcccat ttagaaccta ccggcatcag agtcagtgct tcaatccagc cagggatcac
     6901 gccattaacc gggaattcag cgggttcacc acgagcaacg gagctgtcaa acacggtgcc
     6961 gtcgatcagt ttaccggtgt aatgaacacg aacgcggtcg gtacgtgccg gaattgcgcc
     7021 ttcaccctgg ttgatcacgc ggaattgcag gccagattcg gtgctattca caccttcttt
     7081 tttggcgttt tcttccaggt atttcacacc ttcagcagcc atcgcctgga aacgctgacg
     7141 acgaacggca tcggcgcgtt cgtggatttc acgcagcgcg cgatgcacca catcaaccgg
     7201 cacagccgga tgtttgcctt ccagcgcatc ggcaatacct gcaaccagtg cttctggcag
     7261 cagcccttcc aggccagatt cactcagttg ttgccctacc tgcaaaccaa tgccgtagct
     7321 tgcttgcgct tcgatggtgt caaaagttgg ggtggtcatc gtatttcctt tcttagggta
     7381 taaaagtagc gggcagcata tcagccacac ttctttgggt aaaactttgt cgcaggaatg
     7441 atgacaaatc ccggggaaaa agaaacaata gcaatatccc gtatgaatat aataaaggcg
     7501 ggttggtaat gctgccaact tactgattta gtgtatgatg gtgattttaa ggtgcttgcg
     7561 t
//
LOCUS       DADMEQ010000156         7457 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.158, whole genome shotgun sequence.
ACCESSION   DADMEQ010000156 DADMEQ010000000
VERSION     DADMEQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.158"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            215..291
                     /locus_tag="JNZ05_003603"
     tRNA            215..291
                     /locus_tag="JNZ05_003603"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:249..251,aa:Arg,seq:tct)
     gene            complement(337..1098)
                     /locus_tag="JNZ05_003604"
     CDS             complement(337..1098)
                     /locus_tag="JNZ05_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8566670.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(1281..2171)
                     /locus_tag="JNZ05_003605"
     CDS             complement(1281..2171)
                     /locus_tag="JNZ05_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HAY8566671.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(2172..5144)
                     /gene="nfrA"
                     /locus_tag="JNZ05_003606"
     CDS             complement(2172..5144)
                     /gene="nfrA"
                     /locus_tag="JNZ05_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HAY8566672.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKAGDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTGAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPTNLPRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRELGNNAQYWWLHAQRYILAQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5131..7368)
                     /gene="nrfB"
                     /locus_tag="JNZ05_003607"
     CDS             complement(5131..7368)
                     /gene="nrfB"
                     /locus_tag="JNZ05_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HAY8566673.1"
                     /translation="MDWLLDVFATWLYSLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQAQAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
BASE COUNT         1738 a         1934 c         1950 g         1835 t
ORIGIN      
        1 tttccgttag ggcggtaaat tgtactttga tacatgaaaa tacgggtttt cttgattcag
       61 acgcgcagcg gtgtgcgttt gtttgccgct atagcgaaat aaatcagaaa atcagacgcg
      121 gtcgttcact tgttcagcaa ccagaccaaa agccattgac tcagcaaggg ttgaccgtat
      181 aattcacgcg attacaccgc attgcggtat caacgcgccc ttagctcagt tggatagagc
      241 aacgaccttc taagtcgtgg gccgcaggtt cgaatcctgc agggcgcgcc atttcttctt
      301 catttatgcc cgtcttatcc gtttccgctt tgcccttcac cacatcactt tttgtcgctg
      361 tttggcgaga taattcaacg gtgtcaggct gtaaaacgcc ttaaaaacag agataaagta
      421 cgacgtgctg ctatagccac ataattgcgc cacctgagtg atatttttgt tatccatcaa
      481 taacatctgt acggcgtaac gcatacgaca ctctgtgaca atctggctat agctggtatt
      541 ttcgtttttt aatttctttt tgagcaggct ggggcttaaa catagcgaac tggcgacaat
      601 tcgcagattc cagtaatgct gaatatcgct ttgaataatg cggcagacgc tgtcgcggac
      661 gctgctgcgt aagatataca tcagtagggc aataaaccgc gattgctcaa gaaagttaga
      721 caatacggta aaaagtaatg cgcgcgtcaa ctccgcttcg cagggtttgc tgggttgctg
      781 cttgctgtgg ttggcggcta gccggaacac ttccggtgta cggctatgac aagcgataac
      841 cggggttgcc agccgcgacc agggctttac acaggttaat ttcttattta aaaacaacag
      901 ataatcgttg atgatgttac ggttgatgtg ggtgattaat acgtgattta acgttgagag
      961 ttcaatgacg ttattgttgc acgcgaaggc ggccaggtag tttttcggaa gggtaaacgt
     1021 agcatggtta ttgacgctta cctctacctc tttttcggtc aggatcacca cgcaaggttc
     1081 actgctgctc aattgcattt cgcactcctc agatatcaga aactccgctc aaaggatcta
     1141 tgcttcctgc atgagtgatc ggcccgttcg ccgataacga tcttctttct ttagcatgct
     1201 ttttagcaat taatcttgat ggaattctga cgagagcgaa agaggtaagc caggtcgtac
     1261 ccgacttacc tggaggggat ttaatactcg agaatgccgt gcgcgacggg caaatagcgc
     1321 agagagaaat agagcatgtc tttaccgcaa gaggaacgtt tcgctaacag cgaggcaatt
     1381 tctgcgtccg gtttcggttt cgctgtaaag gttttgcctt tgccggcgac aaacagttca
     1441 taaacaatgc cgcttgctgg ggcggagctt tggcaatcgg cgctggcctg tagataacgt
     1501 tcacgctgtt cagcggtaag tttatccacg ccgctgccat cctgtaccca gacattaact
     1561 ccggttgctt taacttgttc cagcagttgg cgatagccat cgggcgacat gtttccggcg
     1621 aaaaaactac tgatataaac cggttttgct gaaacatcgc taatcagccg ctgcgcgttg
     1681 tttaaccatg ttagcaaagg ttgacgggcg gcttcgctgc gccagttcag gtcgtcaatt
     1741 tccgcgctga tgtaccagcc atccggcgtt atgccaggcg cggcgctcca taatctggct
     1801 tgctggagat cggcagccag caggcgatta agatagcttt ccagcgctgc agacgactgt
     1861 ttctggtgca taaaaaattc cggatcggcg ttcagcccga caatcagctt caggccagtc
     1921 tgttgcgcag ctgcggcacg cttaaacaat aacgtgcgct gttctggctg ggtaaatgca
     1981 tcgccgtaac gggtccattg caaaaccagg gtatcgaagc cttgaaaacg taactgactc
     2041 atcagcccct gccactgggt atcggtaacc tgactatctc ggttttgtgg ttgccagata
     2101 atacctttca tcgcaaagga aaaagggctg accaaaagca gtgtcagcaa tacgaaaatg
     2161 aacttacgca tttaccagtg cactccaatg gtgagaaacg cgttgttgcg ctctccgtta
     2221 cgttgattaa tcgccttaaa ggtatgttga tactcgacgc cgagactgac tttgtgcggc
     2281 caggcgtcgt agtgcgtctc gccggtccaa atattccagc ggaccccgac accgccaagc
     2341 tgcgcgccct gagtgccttt atcacgatag ccgttgtcct gaacgtgagc gtaaggctca
     2401 atagtctgtc cgttagctac cttctgatgc cagctgacgc gataatctgc cgtccacgcc
     2461 tgaatatcct ggcggatata ttgcgccgca tcgaggtaca ggttttgggc aaaccagcct
     2521 gaaccgttcg ggtgccattc gtcgctgtat ttgccgccat taaagaatga ggcgctggcg
     2581 cgcagcatgg tatcggatgc gccattttgc ccgttcagcg gcaactgctg ttcgacggcg
     2641 aggaaaaaga tctgatcgcg cagcggcttc cagcgcagac cggtgccgga catcggattt
     2701 ttcaccggca tcatcacccc gttttctccg gtatcggcaa agacgcggct ataaactgag
     2761 agcaggtcgc cttccagcag catattgcgt ccgatgcggt actcggcttc cagttgtccg
     2821 tagctacgat agcttttccc aggcgctgca ccgccgacat tattgttagc ggtactcatt
     2881 gcgccggaac gcaagccgat ggaagaatcg aaactgaacg tccagcggcg accgacctcc
     2941 tcatgcaaac ggcggaaatt gaagcgttgc tgattttgct ctggggtcag tggggttatc
     3001 agcgcctgat tatcaatgtc atcaatcacc agccgggcgt agtgctgcgt cgcaggcatg
     3061 tcatccagac gctggttcac gtaggccagt tgtcggatca gtgccggatc gtccggaagc
     3121 cctttatgcg cctgttcgag catttcccgc gactgtgcga tatcaccgct atcccacaag
     3181 gcgtaaccaa gcgctgcctg ggtgttgcta ttattcggtt ccagttccag cgcggcgcgc
     3241 aaatcactca ccgcggccgg aacattatga cgttggcgat aaattgtcgc ccgcgcaacg
     3301 taagcgttgg cagaaggggc aatattgatt gagcgcgtga gatcgttcag tgcgagttct
     3361 ggctgagcta ggatataacg ttgcgcatgc agccaccagt attgagcatt atttcccagc
     3421 tcacgttgtt ctgcctgttg aagccagcga tcgcgagccg cgccatttcc cgctgcctga
     3481 gcggtgttgg cggcggcgag cagatcctca ttgctcatgt cgtgaagact gattttctgc
     3541 caggccgcca gtgcggtggc gtagtcctca acctgatacg cctgataggc taccgcacga
     3601 tgttgccagg cgctcggttg tcgttgttcg gcctgaagcc atgcatacaa cgccacaccg
     3661 ggtagcgtgt cccgataaca ctttgccaga cggttccagg cggcagcatc gtaggaaggc
     3721 gacatatcgc ccagcaagcg aactattgcc gggcaattat ctgcaatacc cggcaactga
     3781 ctttgccact gacgttgctc cgccagcggt aagggtttcg ataaaatcgc caccttcgcc
     3841 ggcgttgcta ggtaaggatg actttccagc agagacgcca gtcgcgccat taaagtctgg
     3901 ctgacacgcg catcgccctg gaaaggatag cgttgcagca ataaatcggc agcttcgcgt
     3961 gactgctcgt tctgcatcag ttgccaggtc agttgatcca ggcggggaag atttgtcggt
     4021 tcttgctgat acagcaatcg tgccagacgc agagattcag ccttgttacg ggtcgccacg
     4081 ctgacagcat aacgctcctc aagcatttca ttggcgggga gggtggcgag cagtttttgc
     4141 gctgcgtcgt actgaccttc ttttaacagc accggtagcg tcgcgccaac aacatactgg
     4201 cggttatcgg caaactgcac cgtataattc gccaatgcct gaacggggtt ggcgctgtat
     4261 ttagataaca gatagagcca acttttctct tgtgcgcccg tggtaaacag cggcttattt
     4321 tcaatgagat aatgctggag gcgtgctttt tcgccacgat aagccagcgc ggtcgcgtaa
     4381 gtaatatatg actgaggatc ggtgaagatc ccctgtgatt gcagtgccag gatccgatcg
     4441 tccagctgcc cggcaagaag cacgtcaaac cactgacggc gttctgccgc gcttaatgtg
     4501 ttctgctggc gtgcttcatt gtatagcgta tctgcctggg accattgttt caggtagatt
     4561 gcccgttgca gcagatcggt tcgcagcgtt tttccttccg gcgatgcagc aaacgtcgca
     4621 tcgttcagtt gcgctctggc gacaggtaat tgtgccagcc gcagggcatt ctgcccgact
     4681 tcactgcgac aacgcagggt cggcgcagcg tcgcccgctt tttgctgggc aagcagttct
     4741 tcaacagtcg taacgctttt cacttcaact ggaatagccg ccagactgcg ctcaagtcgg
     4801 gcatctcctg ggtgacgttt cagttgatcc tcaagcaaca gccgcgcccg gtcatcatga
     4861 ccaaaatgac gataggcttc cgcaaggtat aaagtcagcg gaatattatc cggcacctgt
     4921 tggtgtatat attcaaattc gcggatggcg gttgcttcgt cgttattttt ctgtgccttc
     4981 agcgccttat cgagacgggg ataaataaca aaatggcgat aatcgctcag ccccagctct
     5041 tctgcgctgg tgccgatatt gtctgcgagt gcgctggtac tcaataaaga cgtcagcagt
     5101 aaaccagacc atccgatgac gcgattaagg ttattctcct tcattttcgg actccagttg
     5161 cgcaacctgt tctgtgttta aacctgcttt gagtaatagt gattgcatcg aaacttgtaa
     5221 ttcgcgttga attgtcagga cgcgatccaa cgtttcctgg ctgataacgc cttcggtgac
     5281 caaaaacttg ccgagcggca gagaactgcg ttcatggcgc aataacaaca cgttaattgc
     5341 tgaacgatta atatgaccga gcgtggtcag tatttcggcg aacaggaact gatgcggcac
     5401 atattgccgc cagatttcac cggtctgctg ttccgtgagc cactgatgct gaaccgcatt
     5461 gtacaacatt gcccgcggat cgtgaccgcg tcggcgtgca taccagtggc gtaaccctgt
     5521 gacaatttgt ccccgcagaa caatgacgta acgcactttg cgtccgactt tacgcgtcag
     5581 ggccgccagc gaaaccgggt caataccatc ttcactgccg acaattaact catcattttc
     5641 cagacgcagc ggcagtactg cataatgcag cgctacggag gctggcattt cggcaatcag
     5701 cgaggacggg atctgccagg catcgatgga ttcccacgcc acgccgtttt gctctgcctg
     5761 cgcctgtgcc agctgctcgg cgctaatcag cccctgcatc agcattgaac cgcccaggcg
     5821 tagaccttcg acgcgattac gcagtgctgt atcgagttgt tcttcagtga tgacctgatt
     5881 ttccagcaga atttgaccta acgggcgcaa cgagcgggta tcgccagtga cgctggggaa
     5941 gtcatgcgtt gttttatccc acgccacgcg acgcggatcg ccgtgctgaa gaacctgttt
     6001 cagcacgcgc cagttagcca taaagttaat caggttgccc cagaaaagac gcaggacgga
     6061 aagcaggccc tgcgtgaggc cgtagtagcc agtgacgaaa atcactcgct gcacgatgcg
     6121 gttaaccatc aagccaaagt ttagccacag cagggtcatt aaccatgcgc tgccgctaaa
     6181 aatagaaagg aaatgccagg catcgggcca caaactttca tacgccagca acagcaaaag
     6241 ctggatcatc accagcatcg cgaggaagct gacaaagtta ctgattgccc ctttgcggtc
     6301 gcgccagaga aagtagttca gcgtcaggct ggaggtccat ttatgggttt taaagccctg
     6361 gaaaacaatg ccgatgatcc agcgagattt ttgtcgaacc gcagtcgaaa aggtatcggg
     6421 gaaatattcg cgcacgcaga tcatgtttga cgtccgtgcg tgctgtaaaa atttacgctg
     6481 ctcgcgttct ttggcttcgt ccaccaccgg aaaacggaca aaaatttccg tcataccttt
     6541 ttctttcagg cggaagccaa tgtcgtaatc ttcagtaaga ctctgcacgt cgaaagcaat
     6601 accgttaccg tcagctaaca gtgcggtcac ggcgcggcgg ctgaaacagg tgccgacgcc
     6661 tgcgctgggc acttgtccgg cgagggcttc acgcaccgga acatctttgc catgcagttc
     6721 tgaaaactca tcaatgtaag tcatgctggt gaagtgcgtc cattcgcgtt caaacggata
     6781 cactgggatc tgaatcagat ctttacgctc gaccagatag ttgaacagac gcaattccat
     6841 cggtgaaatc acatcttcgg cgtcatgcag aataaaacca gcaaaagcga aattggcgct
     6901 acgttcaaac tgggtgatgg cgtccagcac gttgttcaga cagtcggctt tgctggtggg
     6961 accaggacgc gcgcagacta ccttatgcac gttcggaaag cgagcgcaca cttcgtcaac
     7021 atcacgttga gtatcggggt cgttggggta ggtgccaaca aagatatgat agttttcgta
     7081 gtcgagcgtg gtcgccgcca gctcggccat attgccgatg acgcccgttt cattccacgc
     7141 cggaaccata atcgctaacg gtttttcatc tggtttatac agttcgcggt aactcattcg
     7201 cgggtagcgg cgataaacac tcaacttgcg tttaatgcgg cgtacccagt agacgacatc
     7261 aataaaaaaa tcgtccagcc cgctgatgaa catgatgacc gctaacgtta tcgcgattac
     7321 ttttaagctg tagagccagg tagcaaaaac atcaagaagc cagtccacac aaaaacctta
     7381 cattaacgct ggttatgttt agggtggcgt atattaaggt tttttatgaa ttgtgacagc
     7441 tttttactat taatagg
//
LOCUS       DADMEQ010000157         7376 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.159, whole genome shotgun sequence.
ACCESSION   DADMEQ010000157 DADMEQ010000000
VERSION     DADMEQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.159"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..207)
                     /locus_tag="JNZ05_003608"
     CDS             complement(<1..207)
                     /locus_tag="JNZ05_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566674.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQS"
     gene            727..1422
                     /gene="minC"
                     /locus_tag="JNZ05_003609"
     CDS             727..1422
                     /gene="minC"
                     /locus_tag="JNZ05_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAY8566675.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAGRL
                     QLVENALTVQPLN"
     gene            1446..2258
                     /gene="minD"
                     /locus_tag="JNZ05_003610"
     CDS             1446..2258
                     /gene="minD"
                     /locus_tag="JNZ05_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAY8566676.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2262..2528
                     /gene="minE"
                     /locus_tag="JNZ05_003611"
     CDS             2262..2528
                     /gene="minE"
                     /locus_tag="JNZ05_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAY8566677.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2900..3244)
                     /locus_tag="JNZ05_003612"
                     /pseudo
     CDS             complement(2900..3244)
                     /locus_tag="JNZ05_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3279..3398)
                     /locus_tag="JNZ05_003613"
     CDS             complement(3279..3398)
                     /locus_tag="JNZ05_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY8566678.1"
                     /translation="MPLSYAGYHHFIMMSLVSAEHNTPALSLQDESPGLVPAG"
     gene            complement(3359..3544)
                     /locus_tag="JNZ05_003614"
     CDS             complement(3359..3544)
                     /locus_tag="JNZ05_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566679.1"
                     /translation="MSRRYSSATITLVILFCRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3645..3818
                     /locus_tag="JNZ05_003615"
     CDS             3645..3818
                     /locus_tag="JNZ05_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566680.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3880..4164
                     /locus_tag="JNZ05_003616"
     CDS             3880..4164
                     /locus_tag="JNZ05_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HAY8566681.1"
                     /translation="MADMNRTTKGALLGAGVGLLTGNGVNGVLKGAAVGAGVGAVTEK
                     GRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            4168..4503
                     /locus_tag="JNZ05_003617"
     CDS             4168..4503
                     /locus_tag="JNZ05_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HAY8566682.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4560..6881)
                     /locus_tag="JNZ05_003618"
     CDS             complement(4560..6881)
                     /locus_tag="JNZ05_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY8566683.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINSQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LYLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTETTNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6998..7207)
                     /locus_tag="JNZ05_003619"
     CDS             complement(6998..7207)
                     /locus_tag="JNZ05_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566684.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
BASE COUNT         2021 a         1738 c         1641 g         1976 t
ORIGIN      
        1 tgattgtaag ttttcagcgg ctttttcgcc gagatatttc cttgttaact ctggggaaat
       61 gccatctgag ttcacacaaa tatacgggtc gcaaacaacg ccgcacgccg gagattgaag
      121 tccgccagcg tcatgtccag acgccattga tgagaacatc atccccacac ctgacaatcc
      181 gatataaata atacgcatca tattcatagc acatcctgat aatccaaccg tacaacactt
      241 atgctcattt cgtcatcaat ccgtaatcaa aaccgctgac ctggacgagt tacgatgacc
      301 tgacagaagt atcagacgtc ttctgcggtt tctatgatag agcaattctg tggcggatat
      361 taagataacg ggtatctgtt ctttaatgat gctgcaaaaa atggaaagtt aagaaagcct
      421 ggcgtggtga aaaaatacac ttgcctcaat actggatatt taaacaggtc tggagatgct
      481 gtcacgatta gggtgcggaa aaaggaagcg taaataagaa gaatcaaaag tacgacggca
      541 atgggttgat tgacaagggt attttttaag ctatgaatca gcgccattta tcacagaata
      601 gacttttact ctgaataaat gggagggtga cttgcctcaa tataatccag actataacat
      661 gccttatagt cttcggaaca tcatcgcgcg ctggcgatga ttaatagcta attgagtaag
      721 gccaggatgt caaacacgcc aatcgagctt aaaggcagta gcttcacttt atctgtggtt
      781 catctgcatg aggcagaacc taaggttatc catcaggcgc tgaaagacaa aatcgctcag
      841 gcccccacat ttttaaaaca tgcccccgtt gtactcaacg tcagtgcact ggaagacccg
      901 gtaaactggt cagcgatgca taaggcggtt tcggcaaccg gtttgcgggt tattggcgta
      961 agtggctgca aagatgcgca acttaaagcc gaaattgaaa aaatggggct gcctatcctg
     1021 acggaaggaa aagaaaaagc gccacgtcca gctcccgcac cgcaggctcc agcgcaaaat
     1081 acaatgccgg tcacaaaaac gcgtttaata gataccccgg tgcgttccgg tcagcgtatt
     1141 tatgctccac aatgtgatct gattgttaca agccacgtta gcgctggggc cgaattgatt
     1201 gccgatggga acattcatgt ctatggcatg atgcgcggtc gtgcgctggc aggggcaagt
     1261 ggtgaccggg aaacgcaaat attttgtacg aacctgatgg cggaattggt gtccatcgca
     1321 ggtgaatact ggctgagtga tcaaatccca gcagaatttt atggcaaagc ggggcgactg
     1381 cagttagtcg aaaacgcttt gaccgttcaa ccgttaaatt gatccctttt taacaaggaa
     1441 tttctatggc acgcattatt gttgttactt cgggtaaagg gggtgttggt aagacaacct
     1501 ccagcgcggc catcgccact ggtttggccc agaagggaaa gaaaactgtc gtgatagatt
     1561 ttgatatcgg cctgcgtaat ctcgacctga ttatgggttg cgaacgccgg gtcgtttacg
     1621 atttcgtcaa cgtcattcag ggcgatgcaa cgctaaatca ggcgttaatt aaagataagc
     1681 gtactgaaaa tctctatatt cttccggcat cgcaaacacg cgataaagat gccctcaccc
     1741 gtgaaggggt cgccaaagtt cttgatgatc tgaaagcgat ggattttgaa tttatcgttt
     1801 gtgactcccc ggcagggatt gaaaccggtg cgttaatggc actctatttt gcagacgaag
     1861 ccattattac caccaacccg gaagtctcct cagtacgcga ctctgaccgt attttaggca
     1921 ttctggcgtc aaaatcacgc cgcgcagaaa atggcgaaga gcctattaaa gagcacctgc
     1981 tgttaacgcg ctataaccca ggccgcgtaa gcagaggtga catgctgagc atggaagatg
     2041 tgctggagat cctgcgcatc aaactcgtcg gcgtgatccc agaggatcaa tcagtattgc
     2101 gcgcctctaa ccagggtgaa ccggtcattc tcgacattaa cgccgatgcg ggtaaagcct
     2161 acgcagatac cgtagaacgt ctgttgggag aagaacgtcc tttccgcttc attgaagaag
     2221 agaagaaagg cttcctcaaa cgcttgttcg gaggataagt tatggcatta ctcgatttct
     2281 ttctctcgcg gaagaagaac acagccaaca ttgcaaaaga acggctgcag attattgttg
     2341 ctgaacgccg tcgcagcgat gcagaaccgc attatctgcc gcagttgcgt aaagatattc
     2401 ttgaggtcat ttgtaaatac gtacaaattg atcctgagat ggtaaccgta cagcttgagc
     2461 aaaaagatgg cgatatttct attcttgagc tgaacgtgac cttaccggaa gcagaagagc
     2521 tgaaataagc ccgctgtaaa aacgcattta tcttcaaggc agagtcattt ctgccttgag
     2581 tttttcatcc ctctcatcca cgttgtggta aagcggcgag tattcttgct gatactcctc
     2641 attgctattt ccacctcccc ctttacacct taaggctgtt cccacgcaca gaacaaaatg
     2701 tttatgcctg gttgagtaaa taaccatgtt gttagtgtgg atatactggg agatgataac
     2761 cccgcaattt cattattcat tgttctccca taggatgagg tttcccgccg taactggcgg
     2821 gctttttttg ccccagaatt ttctctttca acatcctgta aacgaaaact gcgccgaagc
     2881 gcagtttaat cgttgatggt cagaacgacc agttcacacc agccaccgcg ttccacgggg
     2941 actccacacc ggcaccatgg ctatacccca ccccgaaatg cccgcttaac gtactgctga
     3001 atgaggcttt aatacctgcc tggtatattc cacgtctgcc cgacaaatca ttgacaaaat
     3061 taccgtcact attcactttc acccgattat catcgacaaa ttctttgcgc acagccgcct
     3121 tcaggcaggg ttcaatttcc ataccgttcc ccagacgcat gttgtaactc agcgttgcac
     3181 ccagttcacg atatatactg cgggtatcga ctgatttcga ttccatgcca ttggataaat
     3241 gatagtagcg tctaagtgac ctaagctctg ctgcaacatc agcccgcagg caccagacca
     3301 ggggattcat cctgaagaga cagcgcaggt gtattgtgtt cagcgctcac caaagacatc
     3361 atgatgaaat gatgatatcc cgcataagaa agaggcattt tttacacgca gtgcgctgaa
     3421 gtgtagttgg ataaaaaatc actcttttca gataatactg tcgtacgctt atctttagac
     3481 aatgaggccg tcggcaaaat aaaatgacta atgtaatcgt tgctgatgaa tatcttctgc
     3541 tcacataaaa atcacacaat aatattgata aatcagatta tcttaccttt acaacattgc
     3601 gttaacagtc aatgacactt cttgaataaa aatattgtca atcaatgagt tattcctcat
     3661 ttaagattat tctcattcac gtaaaaaaca taattcctat catcacagcc acgctcattt
     3721 tgaattatct gaataatagc gagcgttctt tagttaagca gatcttaatt gaagacgaaa
     3781 ttattgtgtg tgcaacttac ttaataccgg acatctaagt tgaagaaaaa aatacttgcc
     3841 ttcgggctta tctcagcatt gttctgctcc acgccagcga tggcggatat gaaccgcacc
     3901 acgaaaggtg cattgctagg cgcaggcgtt ggtttactta ctggcaatgg tgttaatggc
     3961 gtactgaaag gtgcagctgt tggcgctggt gttggtgcag taacagaaaa aggccgcgac
     4021 ggtaaaaatg cgcgtaaagg tgcaaaagtg ggtgccgccg ttggtgctgt cactggcgtt
     4081 ctgaccggca atggcctcga aggggcgatt aaaggtgccg tgattggcgg tacgggtggt
     4141 gcaattctgg gtaaaatgaa ataaggaatg atgatgaaaa aatttgcttt actggcgggc
     4201 ctgtttgttt tcgcaccaat gacctgggcg caggactaca atattaaaaa cggtttgccg
     4261 tcagaaacct atattacttg cgcagaagcc aacgaaatgg caaaaactga cagcgcacag
     4321 gttgcagaaa ttgttgcggt aatgggtaat gccagcgttg ccagccgtga tttaaaaatt
     4381 gagcaatctc cggaactgag cgccaaagtg gtagaaaaac tgaatcaggt ttgcgctaaa
     4441 gacccgcaaa tgctgttgat cacggcaatt gatgacacca tgcgtgctat cggcaaaaaa
     4501 taatatttca tatgtaccgc gtcgatgaat acgacgcggt tttttagcta acggcgggat
     4561 cagaacttat atttcaggcc caccatcact gcggtgtcat tgtagccatt atcgcccagc
     4621 tgcacgccca cattccccca gacgctggcc gctggattca ggttgccatt tacgccaagt
     4681 ttaatttccc ccaaattact caccccatct tgttcgactt tcacaccgtt cattgagaca
     4741 gcaaaatctt tactgttatg gatccagttc atttcaacaa aaggttcaaa gtcgtcaccg
     4801 tgcggaccgc tgtttttctc ctgagtacga ataaaggtgc ggaagccgag tcgggtctgg
     4861 atgttgttat ttgcgtcatt ctcaatacgt gagccattgg cttccgtgtg cgcattatga
     4921 tcgacgccca tccagatagc ctgtgcctgt ggctggaaga tccacgtata tttcgccgct
     4981 tccagaccaa aggatttatt cagggcaaaa ccgtaacccg cttccagcga gacggttgca
     5041 cctttcgcgc tataggattc ataggctaat tcatcccctt tcacggagtt tttaaaccag
     5101 ctatattgcg cccaggagtc aatgtatgcg ccttttttac tgatgtcatc ggcaaaccaa
     5161 gtggcataca gccctgcgct atagcctctg acactccctt tcgaacgata atccgacacg
     5221 ctggaatgag ttaagttgta gtcgcgggca taaccagcca tcactcccaa acgccaactg
     5281 tcactatcgg taaaaccacc ggttaacaga tcgcccccta actgcgagac gtagcgatgc
     5341 gaggtcgttc tcaactgtcc gttgctgtca cgccaggcat tatgcccgcc aatttgacgt
     5401 agccaaagtc ggcttgaacg ctcctgttca gttacaggat cgatgtagcg cgtttcaccc
     5461 gcacggtcgt tcagacgcat aacaaaaaga gtgttagcgg ctgccaggtt ggcgacgtaa
     5521 cttccagctt cggggcgcag gacgggggta ggcgcaacag taggcgctga acttcctccg
     5581 gagtttgttt caccgccgct cgttgcctgg aagttagtca gataccagtt tttattggtc
     5641 gtttcggtat tacctcgggt taatgaatat tcgtaggcac cggcgaaaat cctcgaatcc
     5701 ttaataaatt ccccatttga ttccccctca acgctgataa tctctatccc atttaacgtg
     5761 taagctccgc tgcctcctgc attggtaact ttaactcggg ttgttccaga ggtatttcca
     5821 ttaataacga ctttatccgt agctgaatta tcatcatcca attcagttct gagatacaat
     5881 acgccattat taccaacgta attttcagtt attgttaatc ttgttggttc gacatcccgt
     5941 ccatctgccc gggaaatata gaccgtacta ttatcaaccg tgaggttgct caccgttgag
     6001 tcagctttaa tatcccaggt actattatct gaaagcgaca gataggtatg agtgttatca
     6061 tctgttgaaa tattagctga acccgttatt tttgaattag ttgcatcgac ggtcaaatga
     6121 cctttaatat tattgataaa aacatctgca gccgatgata cctcactatc cgtaatggtc
     6181 acgttactcg tctcattttt atggtagagg atttcatttg ctgaccgtag tgagctacca
     6241 gataaaatat ctaccgttgc ttcctgacta tttatggaag actccagcac aattccagac
     6301 ccctgatgcg cgactgcaga tgtatttttc agcgtcaccg tgccgccatt ataaatttta
     6361 gcaatcgcgg cgtcaggacc cgttgtataa atttctccct ggttaaatga aactgtcccc
     6421 tggcgggcag cgatggcatg agcattgtcc ccatgcgtgg tgatatcaac atcggttaat
     6481 gtaacgctgg cattggtata atcaagcgtg tcaccagcct tgacgacagt gccattagcg
     6541 tacgcggcat aactttctgt tccgtgggtt gtgatctccc cttttgccat atccagtttt
     6601 gctatggcat tattttcatt acttcctgta tcggcagtac tttcttcatc aagagaagat
     6661 acacacgcac gcgcgcttcc gggagatgca cacgcaagaa cgccgactgc gtgattaccc
     6721 gcagtaacaa taaatgtatc atctccgtca gaaaaagagg gggtaaccga accgtcagag
     6781 ttgactctta ttatggtgtc agtattcagg tttccactcg tttgacttag caaaccaatt
     6841 gagtaatccc ccgttgtgac aatcgtgttt ttaccctgca aaataacagc accgccaaat
     6901 tcacttacga ttgcgctagc tgttaatgct gggcttagag gactattgtc agaaggatat
     6961 aaaccgcccg ttgttacaat gacgccattg ttaatggtta acttactacc ctctcctgca
     7021 acggataccc gtaacttcca ttacccgttg ttataactag cgcattatca atatctttta
     7081 cttttccatc ttttattgcc acagcatgtt tatattcatc gtgctgaata aactgttcag
     7141 catatgcatt acccaccgcc agagcaataa gtcccaaact aaacccgggg agttttttca
     7201 gtttcataga ttaaccatcc attcattgga ttaacaaaca ttcctggtca aataacctat
     7261 gagctcagat taccaaaacg gagggtaaag acaaaaacaa aagcacaaaa aatttaaata
     7321 gataaacagc gacaatatat aaatgataaa taagtcggca ttatcatttt tcacct
//
LOCUS       DADMEQ010000158         7237 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.160, whole genome shotgun sequence.
ACCESSION   DADMEQ010000158 DADMEQ010000000
VERSION     DADMEQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7237
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.160"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            27..878
                     /gene="hchA"
                     /locus_tag="JNZ05_003620"
     CDS             27..878
                     /gene="hchA"
                     /locus_tag="JNZ05_003620"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HAY8566685.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(986..2344)
                     /locus_tag="JNZ05_003621"
     CDS             complement(986..2344)
                     /locus_tag="JNZ05_003621"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HAY8566686.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLRELKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLESLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2344..3015)
                     /gene="hprR"
                     /locus_tag="JNZ05_003622"
     CDS             complement(2344..3015)
                     /gene="hprR"
                     /locus_tag="JNZ05_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HAY8566687.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3148..3561
                     /gene="hiuH"
                     /locus_tag="JNZ05_003623"
     CDS             3148..3561
                     /gene="hiuH"
                     /locus_tag="JNZ05_003623"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAY8566688.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3670..4674
                     /gene="msrP"
                     /locus_tag="JNZ05_003624"
                     /pseudo
     CDS             3670..4674
                     /gene="msrP"
                     /locus_tag="JNZ05_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4675..5309
                     /gene="msrQ"
                     /locus_tag="JNZ05_003625"
                     /pseudo
     CDS             4675..5309
                     /gene="msrQ"
                     /locus_tag="JNZ05_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
     gene            5567..6217
                     /gene="zinT"
                     /locus_tag="JNZ05_003626"
     CDS             5567..6217
                     /gene="zinT"
                     /locus_tag="JNZ05_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HAY8566689.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         2053 a         1636 c         1518 g         2030 t
ORIGIN      
        1 aactaagcaa caataaggaa tacactatga ctgttcaaac aagtaaaaac ccgcaggtcg
       61 atattgctga agataatgcg ttcttccctt cagaatattc gcttagccaa tatacgagtc
      121 ccgtctctga tcttgatggc gtggactatc caaaaccgta tcgcggtaaa cataaaattc
      181 tggtgatcgc cgcggacgaa cgttatttgc cgaccgataa cggaaaactg ttctcgacag
      241 gtaaccatcc gattgaaacg ttgctgccgt tgtatcatct ccatgctgca ggtttcgaat
      301 tcgaagtggc gaccatttcc ggtctgatga ccaagtttga atactgggct atgccgcaca
      361 aagatgaaaa agtaatgcca ttctttgaac agcataaatc gttattccgt aatccgaaga
      421 aactcgcgga tgttgtcgcc agcctcaacg ctgatagcga atatgcagca atctttgttc
      481 ctggtgggca tggcgcactt attggtttac cagaaagcca ggacgtggct gccgctttac
      541 agtgggcaat caaaaatgac cgttttgtta tctccctttg ccacggtccg gcggcttttc
      601 tggcacttcg ccacagcgat aacccactga atggttattc tatttgcgca ttcccagacg
      661 ccgcagacaa acaaacgcca gatattggct atatgccggg tcatctcacc tggtacttcg
      721 gcgaagagtt gaagaaaatg ggcatgaaca tcattaatga cgacatcacc gggcgagtac
      781 ataaggaccg taaacgtctc accggcgaca gtccttttgc agcgaatgcg ttgggtaaac
      841 tggcggcgca ggaaatgctg gcagcttacg cgagttaatc cctgttacat tgtgatgtaa
      901 gcgcataatc aatcgcagcc acgttaatac tcacgacgtg gctgcgtttt ttcacggtta
      961 acttatggcg tactgaagcc cgatgttaat ttctttgcgg taacgtaatt cggaacacgt
     1021 tatgcttact gagatagtga tacgtagcac ttcctccatg taattcggca atcgctttga
     1081 ctaaagaaag gccaagtccc tgacctacgg aatgacgcga attatctccc cgccaaaatc
     1141 tacggaagag tttttcaggc tcattaattt tcgttccagg actggcgata tcaatattaa
     1201 gagagccgtt ggcatcaaga aaactggtta tatgaatacg cgatttttct ggtgaatatc
     1261 gaatggcgtt aacaataaga ttcgataaca ttcgttgcag taaaatttta tccgcaaaga
     1321 tttgttgatt gcacttgacc ttaaagcaaa tctctttctc gtctgaaaga gattcaagat
     1381 agtccaaaag attttcaact tccttattga gagaaagcga atccagtttc accaaaacat
     1441 tgtttttgtc tgccctggca agaaacagta tattctctgt taaccgcgaa atattttcca
     1501 gctcttcaat gtttccggca attgtttttt gatattcagc gatacttctg atttgactga
     1561 gcgtaacctg attctgaccc agtaatgcat taattggcgt tctaagttca tgagcgagat
     1621 cgtcagcaaa ttgactaagg cgctcaaaat ctttgactaa agcatgatgc attttattca
     1681 acgcctgccc aagaggtttt agttctctcg gtaatgcgtt aacctcaacc ggcactcggc
     1741 tatcgttata attcagcgct tccgttacgc cactcaactt tttgagctct cgcaaccccg
     1801 tcctgattaa caacggactt aatactgagc acagtataat ggcgacaatg caaattataa
     1861 tgctattaat tttatactgt tcaagcatat tatgtctggc tgaagccaat ttagccacag
     1921 taaccgttaa tgatggcgaa acttcatcaa tgttaattcg taaagcatct atctctgtat
     1981 cattaataat gcttctgtaa atgcctgctg cgctgattgt ctcactagca ggtatgttat
     2041 ttaacatgtc atcactgaca tttgtccggt taacaatttt attgatgcca tcactatgaa
     2101 tgatcaaaat atcctgacta acatccatca tccggttaaa gtacacaggt aacgtatctg
     2161 gatttacccc atcaattaac aactgcttga tctgcgctgt ccggttaatg agtgttgtat
     2221 catcgcgcca tttcaactca cttgccaggc cattatagag agtccataca attccagcgc
     2281 cagcaacaga ctgcagaaat ataaaaagca aggttaaacg gacggttata gataatcttt
     2341 tcattatttt tttaccgcta cgaatgaata gcccatcccc cggattgtgg cgattagctt
     2401 ttcaggaaaa ggatcatcaa cttttgcgcg gagcctgcga atggcgacat ccaccgtatt
     2461 ggtatcacta tcaaagttga ttccccaaat ttcactcgca ataaccgttc tgggtataat
     2521 ctcgccagct ctggaggcca gtagccaaag taactgaaac tccttgcgcg tcagtgtaat
     2581 actgatattg tccctgctga cactttgact gacagagtcc atttttaagc cactgatttc
     2641 taatgttgaa ttcagagcgt gatgttgcct taattgtgcc cgaacccttg ctaacaactc
     2701 agaaaatgaa aaaggtttta ccagataatc atttgcccca ctgtccaacc ctctgactct
     2761 gtcatcgaca gaatcccttg cagtaaggca aataacaggg gtttgctttg ccgttcttaa
     2821 cgtttgtaag atctgccagc catccatacc cggaagcata atatccagaa tgatcaatgc
     2881 ataatcatcc ttcagcgcaa gataaagccc atctctgcca tcagaaacgg catcaatgac
     2941 ataacccgct tcggaaagcc cctgcgttac ccattcctgg gtcctttgat tatcttcaat
     3001 aagtagaatc ttcatgaaaa taatatgcca taaatcatat gttatagaaa caacctggtt
     3061 cattacaaaa ttgtaatgct gctgtaaggt taccctggac gttttttcgc tatcctcaaa
     3121 actcattcac atgacaagga tataaacatg ttaaagcgtt atttagtact ctcggtagca
     3181 acggcagcat tttcattacc ttctttggtt tatgccgcac aacaaaacat tcttagcgtg
     3241 cacattttga accagcaaac aggaaaaccc gctgccgacg tgacagtcac tcttgaaaag
     3301 aaagcggata acggctggtt acaacttaat accgccaaaa cagataagga tggacgaatt
     3361 aaggcactgt ggcccgagca aactgcgact actggcgatt accgtgtcgt atttaaaacc
     3421 ggggactatt tcaagaaaca aaatcttgaa agtttcttcc ctgagattcc cgttgaattt
     3481 catattaata aagtgaacga gcattatcat gtgcctttgt tactcagcca atatgggtat
     3541 tcaacctatc gtggcagtta atgcgtaaat atgtaaaagc tgatctctgc tactgttagg
     3601 gaggtgactc cctgacagat taacagtaaa cagctcttgt tggttaacga caaaaaagtg
     3661 tgatggctta tgaaaaagaa tcaattttta aaagaatcag atgttacagc cgagtcggta
     3721 ttctttatga agcgtcggca ggtgttaaaa gcactgggca tcagcgcagc tgcattttct
     3781 ttgcctcacg ctgcgcatgc cgatctgctt agctggttta aagggaacga tcgtccgccc
     3841 gcccccgccg gaaaaccgct ggagttcagc aagcctgccg cctagcaaaa taacctgcca
     3901 ctgacgccag cagataaagt ctccggttat aacaacttct atgaattcgg gctggataaa
     3961 gacgatcccg cggctaatgc tggtagcctg aaaaccgatc catggacact gaaaatcagc
     4021 ggcgaagtgg caaaaccatt gaccctcgat catgatgatt taacccgtcg cttcccgctg
     4081 gaagagcgta tttatcgtat gcgctgcgtg gaagcgtggt cgatggtggt gccgtggatt
     4141 ggttttccgc tacacaaatt gctggcgctt gccgaaccca ccagcaatgc gaagtatgtc
     4201 gctttcgaaa cgatttatgc gccggaacag atgcctggcc agcaggaccg ctttatcggc
     4261 ggcgggctga aatatcctta tgtcgaagga ttgcgtctcg acgaagcaat gcatccgctc
     4321 acactgatga ccgtaggtgt ttatggcaag gcgttaccac cgcaaaatgg cgcgccggtg
     4381 cgactgattg tgccgtggaa atatggcttt aaagggatta aatcgatcgt cagtattaag
     4441 ctgacccgcg agcgtccgcc aaccacctgg aatctggcag cgcctgacga atacggtttt
     4501 tacgccaacg ttaatccgca tgttgatcac ccgcgctggt cacaggctac cgaacgattt
     4561 attggttcag gcggcattct cgatgttcag cgccagccaa cactactgtt taatggttac
     4621 gccgaccagg tggcatcgct gtatcgtggc ctggatttgc gggagaattt ctaaatgcgt
     4681 ctgacagcaa aacaggtgat atggctgaaa gtttgcctgc atcttgccgg attgttgccg
     4741 tttctctggc tggtctgggc gatcaatcac ggtggactgg gtgccgatcc ggtgaaagat
     4801 attcagcatt ttaccggtcg cactgcactg aaatttttgc ttgcggcctt gttaatcaca
     4861 cctctggcac gctacgcaaa acagccgtta ttgatacgca ctcgccgcct gttaggctta
     4921 tggtgctttg cctaggcgac actgcattta accagttacg cattgctgga gttgggcgtg
     4981 aataatctgg cgttattagg taaggagtta attacccggc cttatttaac gttaggaatt
     5041 atcagctggg ttattttgct tgctttagcg ttcacttcaa cccagtcgat gcagcgaaaa
     5101 ctcggcaaac actggcaaca gttgcataac ttcgtctatc ttgtcgcgat cctggcccca
     5161 atacattatc tgtggtcagt gaagattatt tcaccgcagc cactcatcta cgctgggctg
     5221 gctgtactgc tttagcctta cggtataaga agttgctttc ccttttcaac cggttacgca
     5281 agcaggccca caacaaatta tctctataac ttcatattgc cgacaaactt cgcgttatcc
     5341 cggccggatg cggcgcgagc gccctatccg gcccacacag atcgcatcaa gttatgtttc
     5401 gtttgtcatc aacctttcat caccagaata atttatttta tttcaataag atagttcaat
     5461 acacctgaga ataccatgct ctcatttcct aatgagttgt tgcatttttt catatgttac
     5521 aatataacat tacacataat gtacattaac tctggaggaa attgttttgg cgattcgtct
     5581 tcataaactg gctgttgctt taggtgtctt tattgttagc gctcctgcct tttcgcatgg
     5641 tcatcattct catggcaaac ccttaacaga ggtcgaacaa aaagctgcta atggtgtttt
     5701 tgatgatacc aatgtacaaa accgaacgct cagtgactgg gatggagtct ggcaatccgt
     5761 ttacccttta ctgcaaagtg gcaaacttga tcccgtcttt cagaagaaag cggatgcaga
     5821 taaaaccaaa acatttgctg aaattaaaga ttattatcgc aaaggttatc cgacagatat
     5881 cgaaatgatt ggcattgagg acggcattgt cgaattccat agaaataatg aaacaacatc
     5941 ctgtaaatat gattacgatg gatacaaaat actcacctat aaatcaggca agaaaggcgt
     6001 tcgttactta ttcgaatgta aagatcctga aagcaaagcc cctaaatata tacaatttag
     6061 cgatcatata attgcaccac gaaaatcatc tcattttcac atttttatgg gtaatgattc
     6121 ccagcaatct ttattgaatg aaatggaaaa ctggccaacg tattatccct atcagttgag
     6181 tagcgaagaa gtggtcgagg agatgatgtc tcattgattt ttttgcgctg acttcagtta
     6241 tttaacagaa cagtgcctgg tagtaacaac aataatttac caagacaaat aagtaagaca
     6301 aactcagaca cgctcatact tgagtcgtgt cttttttatt atgtctgtag aaaaaaactg
     6361 agaaaaaggc gaacaataaa tacacaactc agccatacag actggcagcg caaatgtttc
     6421 caggagcaag tcatctgtca tggtaaagct aaccagaaat ataggttaat tttggtgttt
     6481 aaaccttttt tacttgttag tatgatacaa aggctttcaa aaaagctggg aaattcaaca
     6541 actcttggaa gttatggact ttgtcccgcg gctttaatct gacggccgcg ttcctttttt
     6601 ggctcaatta ttgacccctt ctctacgcaa cttcagttcc cgccagcaac tttgcggcag
     6661 tttcatagga tcaatgtcta aaagaataat ggtgaccgat aagaaaacga ctgaataact
     6721 gtagattttc gctcgaaacc ttcctgtcag ctccatagcg aatcaagcgc tgaatgtcac
     6781 agtatcgaac agaaaacagt gacgatctaa cccttcaaga atattctacg attgttctgt
     6841 ttaggaaaag caaggcggga agtcgggaga taagtcattg ataaagtggc ggagagaggg
     6901 ggatttgaac ccccggtaga gttgccccta ctccggtttt cgagaccgat gatacagagt
     6961 cgaaaaatca aagagttaaa aatttttctt ggaaaaaatg tcttaaaatc gtacacgaaa
     7021 aatcaatgaa ttacataaca ttaaagaaga atattccaag ccttcttgag tccaccacct
     7081 gttttcaggt gtcattcaga cgtgatttgc cccagattgg gaaagcacgc ttttttctca
     7141 gcttattccg ttttgcctgt gtgttgaata attccactgc agttatataa aatcggtaac
     7201 ggctggaaat cattcaatac tcgcactatc gaaagtt
//
LOCUS       DADMEQ010000159         7208 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.161, whole genome shotgun sequence.
ACCESSION   DADMEQ010000159 DADMEQ010000000
VERSION     DADMEQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.161"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..329)
                     /locus_tag="JNZ05_003627"
     CDS             complement(<1..329)
                     /locus_tag="JNZ05_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566690.1"
                     /translation="MAYRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQ"
     gene            699..1649
                     /gene="corA"
                     /locus_tag="JNZ05_003628"
     CDS             699..1649
                     /gene="corA"
                     /locus_tag="JNZ05_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HAY8566691.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1690..2070)
                     /locus_tag="JNZ05_003629"
     CDS             complement(1690..2070)
                     /locus_tag="JNZ05_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HAY8566692.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2084..2500)
                     /locus_tag="JNZ05_003630"
     CDS             complement(2084..2500)
                     /locus_tag="JNZ05_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566693.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRRRYIFSFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2559..3449)
                     /gene="rarD"
                     /locus_tag="JNZ05_003631"
     CDS             complement(2559..3449)
                     /gene="rarD"
                     /locus_tag="JNZ05_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HAY8566694.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3501..3968)
                     /locus_tag="JNZ05_003632"
     CDS             complement(3501..3968)
                     /locus_tag="JNZ05_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HAY8566695.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4133..5002
                     /gene="pldA"
                     /locus_tag="JNZ05_003633"
     CDS             4133..5002
                     /gene="pldA"
                     /locus_tag="JNZ05_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HAY8566696.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5135..6964
                     /gene="recQ"
                     /locus_tag="JNZ05_003634"
     CDS             5135..6964
                     /gene="recQ"
                     /locus_tag="JNZ05_003634"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAY8566697.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7028..>7208
                     /locus_tag="JNZ05_003635"
     CDS             7028..>7208
                     /locus_tag="JNZ05_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HAY8566698.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGL"
BASE COUNT         1852 a         1766 c         1804 g         1786 t
ORIGIN      
        1 tgctgaccgt tttcgatgta caaaccgagc ggcgcatagc tttcatcata gatgccgccg
       61 ttcatcgcca tctgcacctg accctgacta ttaatatccg ccagcagagc atgtaacgtt
      121 ccccacgctt cgccattggc tttttgccag tacattttca cccgctctgt ttgaggatta
      181 acggtatacg cctgtacggt cagcgtcgga tctgagagtg cgcaatcatc agcggcaaca
      241 gcaaacaagg gaagtaaggt gagggtgaga aaaatccgtt tgagattcaa ggtgatcatt
      301 cctttaccaa tgagtagccg atacgccatt ataggtcctg gatgtgggat ttttttatcc
      361 tgttagcgac cttgacgagt accaaaaagc gcgaagttca actattgttc tgtggtgttc
      421 tgttgcgtgt tgacggcaaa attttgctgg cgtaacatgc gcgcacgatc actctaagag
      481 gacattcgcc ttggacacac ccagtagata ctggctcact atcctgtcat ccaggatcaa
      541 ctcctaaggc tatccctttt tgctgatagc cttagcggtt gtcagcgacc ttaatttttc
      601 ccgtcgcgct gagtcaggct gtttaatggt ctgaaaccca atttgtttct gtgtgcccac
      661 cgaactgtcc gatattttaa gcattgggag tcccggtcat gctgagcgca tttcaactgg
      721 aaaataaccg actgacccgg ctggaagtcg aagagtcaca accccttgta aatgcagtat
      781 ggattgatct tgtcgaaccg gacgacgacg agcgactgcg cgtacaatct gaacttggcc
      841 agagcctggc aacccgcccg gaactggaag acatcgaagc atcggcacgt ttctttgaag
      901 acgacgacgg cctgcatatt cactccttct tcttctttga agatgcggaa gatcacgccg
      961 gtaactccac tgtggcattt accatccgtg atggtcgtct gtttactctg cgtgagcgtg
     1021 aactgcccgc ttttcgtctg tatcgtatgc gtgcccgtag ccagtcgatg gtagacggta
     1081 acgcctacga gttgctgctg gatctgttcg aaaccaaaat cgaacagttg gcagatgaaa
     1141 ttgaaaatat ctatagcgac ctggagcagt tgagccgggt gattatggaa gggcatcagg
     1201 gcgatgagta cgacgaggcg ctctccacgc tggcggaact ggaagatatc ggctggaaag
     1261 ttcgcctgtg tctgatggat acccagcgcg ctctcaactt cctggtgcgt aaagcgcgtt
     1321 taccgggtgg gcaactggag caggcgcgtg aaatcctgcg cgatatcgaa tccctgctgc
     1381 cgcataacga atccctgttc cagaaggtga acttcctgat gcaggcggca atgggtttta
     1441 tcaacatcga gcagaaccgc atcatcaaaa ttttctcggt ggtatccgtc gtgttcctgc
     1501 cgccaacgct ggttgcttcc agctacggga tgaactttga gtttatgcca gaactgaagt
     1561 ggagtttcgg ctaccctggc gcgattatct ttatgatcct cgcgggcctg gcaccgtatc
     1621 tgtactttaa gcggaagaac tggttgtaaa aagaaggcgg tggcttagcc tggctaagcc
     1681 acctgttatt caaaggctcc aggtatttaa cccttttacc tctttctcat aaaaccattt
     1741 gttcgtgtta acagcaacat aggctgctac ggcaattccc agaatgttaa cgccaattaa
     1801 tgcaccaaca aatagagata gaatgccaag taatagaact ataattgctt ttttccataa
     1861 gcccagaaca aataaataga tccaggaaca gaagaaagca atgaaattca tttgaatagt
     1921 taatcgttgt cttactttta atgctttaaa tgctgcttta tattctggtg ttgctcccca
     1981 gaatccagga aagccatgtt gatcataaaa attaaatcgg tatttccatt tttcacttaa
     2041 tgaatcatcg ttcatatatt ccttactcat aaatactcca taactattgt tttgatgaat
     2101 cagtaggtgc aagcattagc atactgaaag tggaaaaata acaaatcaaa aaaatcatcg
     2161 aaccattgcc tgaacaggca aaatcttcgg ctatcattgt gatgatagag atgatatata
     2221 ctgctaatgt accaaaaaca taaattttta tatatatgaa accactatca cggagtcgtt
     2281 ggcaattcat gttgatgacg agataatgga gtgcgatggt agagactata acaagaaaac
     2341 ctgcttcccc atcgttaaaa aagataataa gaaaggcaaa aatgaaattt attaaaataa
     2401 atgaaaatat atatcggcgt ctggaaatct taccgttaga tgttgggata aacatacgta
     2461 acataaattt tacatccttg tgtgagtctc ctgtcagcat ggcaatatgc ccactctcat
     2521 acaagagtcg gcatattttt tcagaatata tttatttttt atttggacgt tctacgctgc
     2581 gtataaatcg cgtccatcac aaaaattgcc agcgccaccc aaataaaggt gaaagtcacc
     2641 atcttatcgg cacccggttt ttcaccataa aacgtcacag ccagcaggaa catcagcgtc
     2701 gggccaatgt actgaaaaaa gcctaacgtt gagagacgca ggcgcgtggc agcggcagta
     2761 aaacacaaca gcggtacggt agtgacaata ccggcggcga tcagcagtaa attcagcgac
     2821 atcgggtttt gccccatatg gctggttgaa ctgtcggcaa tagcaaacag gtaaatcgcc
     2881 gccacgggca gcagccacat ggtttcaatt aacatgccgg tttgtgcttc taccgcaatc
     2941 ttcttgcgca ccaggccgta gaaggcaaaa ctaaatgcca gccccagcgc gataataggt
     3001 agcgaaccaa aagtccacag ctggaccaac acgccacata tcgccagaat caccgccagc
     3061 cattgcatcc ggcggaatcg ctcgccgagg aaaatcatcc ccagcacaat gttcaccagc
     3121 gggttaataa agtaaccaag gctcgcttcc agcatatggt gattgttcac cgcccagata
     3181 aacagtagcc agttgccacc aatcagcacg gcagagactg ccagcataaa aattttctgt
     3241 ggcgtctgaa tcagcgtttt taaataggac cactggcggc aaatgctcat cagcaccacc
     3301 ataaagaaaa acgaccagat cacgcgatgc gtcaggatct catcggcggg cacgtagtaa
     3361 atcagcttga agtatgctgg cgcaataccc caaataaaat aagcggcaag agcgagtaat
     3421 acgccctgcc gcgtttgttt tgcatccatc gggaatactc atttttaatt ggtaacagca
     3481 gtttacctgc ttttatgtct tcaacctacc atataggtgg cggtggcact ggcaatataa
     3541 agctgttctt cattgtgtaa ttcaacgcgg gcgacggcga ctttattgcc tgcacgcaac
     3601 aggctactgg tagcagtaaa acgctcgccc ctgcctgggc gcagataatc aacgcgaaga
     3661 tcaatggtcc ccatccgcga tagccgctgg cgtagttcat cttcactgat ggtttcgtgg
     3721 cgggttaagg tacttcccac gcacaccaga ccggcggcga catccagcgc cgacgcaatg
     3781 actccgccgt gcaaaatgct ttgcgcccag ttacccacca tcatcggctg atttttaaag
     3841 gccagctgtg cgaactcttt ttcgtaacgc tccagttcca tccccaatgc gcggttaaat
     3901 ggcatgtgat aaacaaacat ctcacccact aatttcaggg cttgttcagc ggtcagtacg
     3961 gcagacatat catccttaca cttcattggt taatgaaatg ttgattttat gcttctttat
     4021 tgttggtttc tactttagga agggataact aacggcaatg gagttaagta tgtaaaatag
     4081 cccgcagaaa aacattcacc ttatcaataa ttcgttacgg agaacacgac cgatgcggac
     4141 tctgcagggc tggttgttgc cggtgtttat gttgcctatg gcagtatatg cacaagaggc
     4201 aacggtgaaa gaggtgcatg acgcgccagc ggtgcgtggc agtattatcg ccaatatgct
     4261 gcaggagcat gacaatccgt tcacgctcta tccttatgac accaactacc tcatctacac
     4321 ccaaaccagc gatctgaata aagaagcgat tgccagttac gactgggcgg aaaatgcgcg
     4381 taaggatgaa gtaaagtttc agttgagcct ggcatttccg ctgtggcgtg ggattttagg
     4441 cccaaactcg gtgttgggtg cgtcttatac gcaaaaatcc tggtggcaac tgtccaatag
     4501 cgaagagtct tcaccgtttc gtgaaaccaa ctacgaaccg caattgttcc tcggttttgc
     4561 caccgattac cgttttgcag gttggacgct gcgcgatgtg gagatggggt ataaccacga
     4621 ctctaacggg cgttcagacc cgacctcccg cagctggaac cgcctttata ctcgcctgat
     4681 ggcagaaaac ggtaactggc tggtagaagt gaagccgtgg tatgtggtgg gtaatactga
     4741 cgataacccg gatatcacca aatatatggg ttactaccag cttaaaatcg gctatcacct
     4801 cggtgatgcg gtgctcagtg cgaaaggaca gtacaactgg aacaccggct acggcggcgc
     4861 ggagttaggc ttaagttacc cgatcaccaa acatgtgcgc ctttatactc aggtttacag
     4921 cggctatgga gaatcgctca tcgactataa cttcaaccag acccgtgtcg gtgtaggggt
     4981 tatgctaaac gacttgtttt gatgaacggt tgagtggttg gcaaatctgg aatccagcat
     5041 ccaggattac cctctcagag actaaaagca ttgcagtttc tcgcgcaggc gctgaaaata
     5101 gcgcctgttt ttatttcagg caatcggggt gaatgtggcg caggcggaag tgttgaatct
     5161 ggagtccgga gctaaacagg ttttacaaga aacctttggc taccaacagt ttcgccccgg
     5221 ccaggaagaa attatcgaca ctgtgctttc cggtcgcgat tgcctggttg ttatgcccac
     5281 tggtggcgga aaatcccttt gctatcaaat tcctgcctta ttgctaaacg gccttaccgt
     5341 ggttgtttca ccgctgattt cgttgatgaa agatcaggtg gatcaactac aggccaacgg
     5401 cgtggcggcg gcgtgcctta actcgacgca aacccgcgaa cagcaacttg aagtgatgac
     5461 aggctgccgc accgggcaaa ttcgcttact gtatatcgcc ccggaacgcc tgatgctgga
     5521 taactttctt gagcatctgg cgcactggaa tccggtgtta ttagccgtcg atgaagcgca
     5581 ctgtatctcc caatggggcc acgatttccg cccggaatat gccgcgctcg gtcagttgcg
     5641 ccagcggttc ccgacgctgc cgtttatggc gctgaccgcc acagccgacg acaccacgcg
     5701 ccaggatatc gtgcgcctgc tggggctgaa cgatccgctg attcaaatca gcagttttga
     5761 ccgtccgaat attcgctaca tgctgatgga gaaattcaaa ccgctcgatc agttgatgcg
     5821 ctacgtgcag gaacagcgcg gtaagtctgg cattatctac tgcaacagcc gggcgaaagt
     5881 agaagacacc gctgcgcgcc tgcaaagcaa gggtattagc gcggcggcct atcatgccgg
     5941 gctggaaaat aatgtccgcg ccgacgtgca ggagaaattc cagcgcgatg atctgcaaat
     6001 tgtggtggcg acggtggcgt tcggcatggg catcaataaa ccaaacgttc gcttcgtggt
     6061 ccactttgat attccgcgca atatcgaatc ctattatcag gaaaccggtc gcgccgggcg
     6121 tgatggcctg cctgcggaag cgatgctgtt ttacgatccg gctgatatgg cgtggctgcg
     6181 ccgttgtctg gaagagaagc cgcaggggca gttgcaggat atcgagcgcc acaaactcaa
     6241 tgcgatgggc gcgtttgccg aagcgcaaac ttgccgtcgt ctggtattgc tgaactattt
     6301 tggcgaaggg cgtcaggagc cgtgcgggaa ctgcgatatc tgcctcgatc cgccgaaaca
     6361 gtacgacggt tcaaccgatg ctcagattgc cctttccacc attggtcgtg tgaatcagcg
     6421 gtttgggatg ggttatgtgg tggaagtgat tcgtggtgct aataaccagc gtatccgcga
     6481 ctatggtcat gacaaactga aagtctatgg tatgggccgt gataaaagcc atgaacattg
     6541 ggtgagcgtg atccgccagc tgattcatct cggcctggtg acgcaaaata ttgcccagca
     6601 ttctgcccta caactgacag aggccgcgcg cccggtgctg cgcggcgaat cctctttgca
     6661 acttgccgtg ccgcgtatcg tggcgctcaa accgaaagcg atgcagaaat cgttcggcgg
     6721 caactatgat cgcaaactgt tcgccaaatt acgcaaactg cgtaaatcga ttgccgatga
     6781 aagtaatgtc ccgccgtacg tggtgtttaa cgacgcaacc ttgattgaga tggctgaaca
     6841 gatgccgatc accgccagcg aaatgctcag cgttaacggc gttgggatgc gcaagctgga
     6901 acgctttggt aaaccgttta tggcgctgat tcgtgcgcat gtcgatggcg acgacgaaga
     6961 gtagtcagca gcataaaaaa gtgccagtat gaagactccg taaacgtttc ccccgcgagt
     7021 caaatgtatg ttgatgttat ttctcaccgt cgccatggtg cacattgtgg cgcttatgag
     7081 ccccggtccc gatttctttt ttgtctctca gaccgctgtc agtcgttccc gtaaagaagc
     7141 gatgatgggc gtgctgggca ttacctgcgg cgtaatggtt tgggctggga ttgcgttgct
     7201 tggcctgc
//
LOCUS       DADMEQ010000160         6930 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.162, whole genome shotgun sequence.
ACCESSION   DADMEQ010000160 DADMEQ010000000
VERSION     DADMEQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.162"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..789)
                     /locus_tag="JNZ05_003636"
     CDS             complement(<1..789)
                     /locus_tag="JNZ05_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M15"
                     /protein_id="HAY8566699.1"
                     /translation="MTQYSSLLRALAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQVLKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLG"
     gene            1036..1662
                     /locus_tag="JNZ05_003637"
                     /pseudo
     CDS             1036..1662
                     /locus_tag="JNZ05_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            complement(1659..1865)
                     /locus_tag="JNZ05_003638"
     CDS             complement(1659..1865)
                     /locus_tag="JNZ05_003638"
                     /inference="COORDINATES: protein motif:HMM:NF019607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY8566700.1"
                     /translation="MAAKIIYWALKDKDVIVMSVNGDKVTEDGRILTDRGQRIRDVRT
                     GPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(1835..2773)
                     /locus_tag="JNZ05_003639"
     CDS             complement(1835..2773)
                     /locus_tag="JNZ05_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY8566701.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGR"
     gene            complement(2884..3267)
                     /gene="bssR"
                     /locus_tag="JNZ05_003640"
     CDS             complement(2884..3267)
                     /gene="bssR"
                     /locus_tag="JNZ05_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HAY8566702.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3480..4805
                     /gene="rimO"
                     /locus_tag="JNZ05_003641"
     CDS             3480..4805
                     /gene="rimO"
                     /locus_tag="JNZ05_003641"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HAY8566703.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNIKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4852..6180)
                     /locus_tag="JNZ05_003642"
     CDS             complement(4852..6180)
                     /locus_tag="JNZ05_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY8566704.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVESINLSTSIGVAHSNKCTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6188..>6930)
                     /locus_tag="JNZ05_003643"
     CDS             complement(6188..>6930)
                     /locus_tag="JNZ05_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY8566705.1"
                     /translation="AMEIGELFLVYQPIVDINTRAILSAEALCRWVSAERGIISPLKF
                     ITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERF
                     TTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKR
                     FYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKL
                     GCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         1811 a         1767 c         1595 g         1757 t
ORIGIN      
        1 ccccagcact acggagatta aacgcatatc accctgggta gccgaagcaa ccagattata
       61 tcccgcacct gccgtggttc ctgtcttcat gccatcaaca ttcagattgc tgctccacag
      121 cagacggtta cggttaggct gacgaatttt gttgaaggtg aactctttct ctttatgaat
      181 ggcgtactct tccggcacat cgtggatcaa tgctttaccc agcaatgcca tatcgcgcgc
      241 ggtactgaac tgccccggcg catccaggcc gtgcaccgtc tggaaggtag tgttggtcag
      301 ccccagtttt ttggcataac cattcatcag accaataaag gactcctggc tcccggcaac
      361 gtaatcggcc agcgcaatac aggcgtcatt accggactgg ataatcacac ctttgttcaa
      421 gtctgccacc gaaacctgat cgcccggttt gaggaacatt accgatgaac cacgcagtgc
      481 cggatttccc gtcgcccagg catctttacc gaccgtcacc atatcggtga gtttaatctt
      541 atcggcctta agcacctgcc caaccacata gctggtcatg attttagtca ggctcgcggg
      601 atccagtttc tcatccgcat tgccttctgc cagcacttta ccgctggcgt aatccattaa
      661 aatccatgca cgcgcatcca cgctcggcgc ctcaacggtt tgttcggccg cgaatgccgt
      721 tggggcaaaa aggaataaaa atgcagaacc cgctgcaaga gcacgaagga gagaggagta
      781 ttgcgtcata aatgccaccc gagtatccat tcaaaaaaaa tacgttatat cgccgtatga
      841 taagttcggt gattaataac gcactcacaa acttaaagaa accgtaagtt ggtaaagttt
      901 ttaaagttta tgcaatatcc tgccgcttcg ctgcttctgg tgtcattttt ttgccagctg
      961 ttgcttaaaa gttaacttta cttcaccatc ggagtcctgt tcagtctgtg gctgcctgtc
     1021 ggaaaagggg tgagtatgat tacgctgtgg ggtcggaata attcaactaa cgtaaaaaaa
     1081 gtattgctga cgctcgaata actggaacta ccttatgagc aaattctcgc gggccgtgag
     1141 tttgggataa atcacgatgc tgattttctg gcgatgaacc ctaacgggct ggtgccgttg
     1201 ttacgtgacg acgaaagtga tctcattctt tgggaatcaa acgccattgt ccgctatctg
     1261 gcggcacagt acgggcaaaa acgcctgtgg atcgactcac cggcacgtcg tgcggaagcg
     1321 gaaaaatgga tggactgggc aaaccagacg ctcagtaatg ctcatcgcgg gatcctgatg
     1381 ggattagtca gaacaccacc ggaagagcgc gatcaggccg ccattgatgc cagttgcaaa
     1441 gagtgcgacg ccctgtttgc cctgctcgat gcggaactgg caaaagtaaa atggttctct
     1501 ggcgacgagt ttggtgtggg cgatatcgct atcgcaccgt tcatctacaa tttgtttaac
     1561 gttggcctga cctggacacc gcgtccaaat ctgcaacgct ggtatcagca actcactgaa
     1621 cgccccgcag tgcgcaaagt ggtgatgatt cccgttagct aattgcgtgg gctaacttta
     1681 agtaattccc cactggactc gtcggtgaga acgtataaat aaccgtcggg tccagtgcga
     1741 acatcacgaa ttcgctgccc tctgtccgtt aaaatacggc catcttctgt cactttgtcg
     1801 ccgttgacgc tcatcacaat gacatcttta tctttcagcg cccaataaat aatttttgct
     1861 gccactgggg gaatttatcg ctgttataga aggccatgcc gctcacagcg ggcgaatctt
     1921 tccagtaaaa aacaggttgc tcggtcccgg cgacgatctc ccctttcgct tccggtatct
     1981 taaagcctga atagttgatt ccccaggttg ccagcggcca gccgtagttt ttgccttttt
     2041 gcgggatatt aatttcatca ccaccgcgcg ggccatgttc attcagccac agtgcattac
     2101 tccacggatt catcgccatt ccttgcggat tacgaatgcc ataagaccag atctcggcgc
     2161 gcgcaccgga ttcctttata aaaggattat catccgggat ttcgccctgg tcggtcagac
     2221 gcaccagttt gccctgtaat ttatccagat cctgcgccgt cgggcgctga ttgttttcgc
     2281 ccagagcaat aaaaagataa cctttaccgt cgaataccag ccgcccgcca aaatggttgc
     2341 cggtagacag ttttggcatc tggcggaaga cggtgcggaa gtcggtcact tttgagagat
     2401 catcacttaa gcggccataa cccacagcag ttccggcttt gccatcatcg ccaacttcgg
     2461 aataacttaa ccagatgcgg cgagactgag caaaatcagg cgctaaaacc acgtccagca
     2521 ggccgccctg cccgtgcgcc caaacgtccg gaactccgga aagcggcgca gataatcctt
     2581 ttcctgcttg ccagtgacgc aactcgccgc cgcgcagagt gattaacata ccgtgattat
     2641 cgggtaaaaa ggccagtgcc cagggatggt cgagtttgtc ttgcagtact tcgacattta
     2701 ccgttgcagg agccgcccag agagcggaag aaagacaaat aaggggcaca aggaaaaagg
     2761 attgtcgatg cataacgcct ccggattgac tctggcttaa agcgtagtca gtggaggaga
     2821 taacaaattc atttttacaa aaacttaaac atgaaggggg agacgctttc tcccccttag
     2881 ttttcaggcc ttctcaagca tggcgtgctt ctgcaggctc tggatactca gcgttaagct
     2941 catcagacaa ttttcaagct tatcggcgtt gacggtaata acagtcgggc aatcatggtg
     3001 cccactcatc aaacatactg cggctgtcgc taatgcttct tcagcatgat gaagagcact
     3061 ccactcttcc tgatccagat gaagattcaa ccgcagcgat ttatcgtgca gttcgcgatt
     3121 cagtttaaaa aagttatctc gtagatgatt gctttcgctg acggacatgt atccttttgc
     3181 cttcctcagt tgcacgtatg cagcgaggtc aacgcgtgcg tcgatcaacc ggttcagcag
     3241 atcgattcgc tgtctgtcaa cgaacatact tcgatcctcc tctttccagc cttgttgcaa
     3301 ttaaatagta tggctatttt ttgagcaaaa tgctgtctag gatcaattct tcacgccgaa
     3361 ttaacaaaat ttacatttcc ttcgcctccg ccagcgaatg agattgcgat acaaatttta
     3421 tccagaactc tgtagaatcc ctgccctgac ctttcctgta actgaatatt ttttaagcta
     3481 tgagcaaagt aactccccag ccgaaaatcg gctttgtttc ccttggctgt ccgaaaaacc
     3541 ttgtcgattc agagcgtatt ctcaccgaac tccgcactga aggttatgac gtggtaccga
     3601 gctatgacga tgcggacatg gtgatcgtca acacctgcgg ctttattgac agcgcggtac
     3661 aagaatcact ggaagccatt ggtgaagcgt tgaatgaaaa cggcaaggta attgtgaccg
     3721 gttgtctggg ggcaaaagaa gatcagatcc gcgaagtcca cccgaaagtg ctggaaatca
     3781 ccgggcctca tagctatgag caggttctgg agcacgttca tcactacgtg ccaaaaccga
     3841 aacacaaccc attcctgagc ctggtgccag aacaaggtgt gaaactgacg ccgcgtcatt
     3901 atgcctatct gaaaatttcg gaaggctgta atcaccgctg caccttctgc attattccgt
     3961 ctatgcgcgg cgacctggtg agccgtccga ttggcgaagt gttaagtgaa gcgaaacgtc
     4021 tggtagatgc gggcgttaaa gagattctgg tgatctcgca ggatacttcc gcctatggcg
     4081 ttgatgttaa acatcgtact ggcttccaca acggcgagcc ggtaaaaacc agcatggtca
     4141 gcctgtgcga acagttatcg aaactgggga tctggacacg tctgcactac gtttaccctt
     4201 atccgcatgt ggacgacgtc atcccactga tggcagaagg caaaatcctg ccgtatctgg
     4261 acattccgtt gcagcacgcc agcccgcgca ttctcaaact gatgaagcgt ccgggttctg
     4321 tagatcgcca actggcgcgc atcaaacagt ggcgcgaaat ctgcccggaa ctgaccctac
     4381 gctcaacctt tattgtcggc ttccctggcg agacggaaga agatttccag atgctactcg
     4441 acttcctgaa agaagcacgt ctggatcgcg ttggctgctt taaatacagc ccggttgaag
     4501 gtgcagacgc caatgccctg cctgaccagg ttccggaaga agtgaaagaa gaacgctgga
     4561 accgtttcat gcagctgcag cagcagattt ccgccgagcg cctgcaagag aaagtgggcc
     4621 gtgaaattct ggtgattatc gacgaagtgg acgaagaagg cgcgattggt cgcagcatgg
     4681 cagatgcacc ggaaatcgac ggcgcggttt atctcaatgg tgaaaccaac attaagccgg
     4741 gtgatatcct gcgtgtgaaa gtcgagcacg ccgatgagta cgatttgtgg ggtagccggg
     4801 tttaattaat tcaggccggg caaaatcatt tgtccggcct gcgaataatg attactgcca
     4861 gctaatctgt gccctgccgt ttttcttact cttatacagc cgctcatccg ccagcatttt
     4921 caagcgttcg acggtagtac atttatttga atgcgctaca ccgatactgg tgcttaaatt
     4981 aatgctttcc acctggaaat gaacgatttc ttttagtatc gatagcaact cttccagcgt
     5041 gccgccagta taaatcaccg caaattcgtc acctccgaag cggtagactt tacctttatc
     5101 taccgggatc tgtgatttaa ggattttcac cactttcatc aacacttcat cgcccagcaa
     5161 atgaccgaag gtgtcgttaa tgtttttgaa tttatcgacg tcaaacagac acaaataacc
     5221 gcctttttcc ggcagcgctt ttaaatcttc atcaaaggat tttctgccca ttgctttggt
     5281 caggtcgtcc aggaacgccg agttatacat actgtagagt tttttgacat cggcagccga
     5341 aaagaaaagc ccgccatcca gtacgcgttc aaaatggtta ttaatgatgg cttctttctc
     5401 cacaatacca cgattaatca ctctgcgtac cagatagagg caactggcac ccgatatcac
     5461 tgccaccaat gcggcaaaga aaataatcaa tgtacaaacc cgcaaataaa catctttttt
     5521 atacgcaaat acgtacaatc cggtcggcaa ataggcgaag tgctccatcg cttccgaaac
     5581 ggtgaagatg gcgtttttag cattatattc atcaccggtc accggtttac cgttcttaaa
     5641 tagagagaac atcaggttct cgctgtccat cgccagcgat tttgacagta aagaaaaacg
     5701 ctccagacca agcaaaaata tcagactacg attgtgagtt accggataga ggtaatagac
     5761 gaaggtttta ccatcaaaag agataaaccc tgtatttttg acatgtcgat acttcacaac
     5821 ctgcataata tctgcacaat aatttttctc gaactccttt acctgaatac attcgtcact
     5881 ggaaatcaga taaggaccga catcgtacaa ataagagggg gaaaccgagt cggcgtaagc
     5941 tgtgcgcaaa ccaaccaaca gctgttcctc cgtgggattt agcgtgttga taagatcaat
     6001 tgccacgcta cgccgatatt cagcaatatt tgccaccgca aaagcagtat caataacgga
     6061 ctgtttcagg ctatacaccc gttcctttac catttgagtg tagatcccgc aggcaactgc
     6121 tgaaatcatg ataaaaatca gcagactgac tgtaagtacg aacttattga ttctggacat
     6181 acgtaaacta ctcttttact aattttccac ttttatccca ggcggagaat ggcatgggtt
     6241 tctggtacag atacccctga gcctgaacac atcctaattt aattaattcg cgcgcaacgt
     6301 cttcgctctc tacgccttcc gcaatcaccg ggatccggtt atagcgtgcc agatcgaaaa
     6361 taatgcgcac aatttttccc gcagtaccga aaatatcgcc ggtaaattgc gagtccacct
     6421 ttatgctgtc tggattaatt tcataaaaac gtttcaggtt tgacaaaccg gtgccaaagt
     6481 catcaatcga tatccgtacc ccttgtttac gcagttgttc aatattgaga taaaaatgtt
     6541 cattaattcg ctcgatcacg gtttcagtga tctcaacaca gaggctgttc gccttcaggc
     6601 cattttcttt catgatggtg gtaaaacgct catgaaagtg cggttcgttt aactgccagg
     6661 gcgaaacatt aatatgcagt aagaaatcat ccttcagcga cgcacgttga ctaaaatgtc
     6721 tgaattcacc cattgccgtt ttaataatct gataacccag ctcattgata aacccgatat
     6781 cttcagcaat ggtaatgaac ttcagcggtg aaatgatccc ccgctccgca gaaacccaac
     6841 ggcacaacgc ctccgcgctc agaatggcgc gggtgttaat atcaacgatc ggttgataga
     6901 cgaggaatag ttcgccaatt tccattgcat
//
LOCUS       DADMEQ010000161         6894 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.163, whole genome shotgun sequence.
ACCESSION   DADMEQ010000161 DADMEQ010000000
VERSION     DADMEQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.163"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..90)
                     /locus_tag="JNZ05_003644"
     CDS             complement(<1..90)
                     /locus_tag="JNZ05_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleE"
                     /protein_id="HAY8566706.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRV"
     gene            146..298
                     /locus_tag="JNZ05_003645"
                     /pseudo
     CDS             146..298
                     /locus_tag="JNZ05_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1679..2626
                     /gene="icsP"
                     /locus_tag="JNZ05_003646"
     CDS             1679..2626
                     /gene="icsP"
                     /locus_tag="JNZ05_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HAY8566707.1"
                     /translation="MKSKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3188..3560)
                     /locus_tag="JNZ05_003647"
                     /pseudo
     CDS             complement(3188..3560)
                     /locus_tag="JNZ05_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3563..3760
                     /locus_tag="JNZ05_003648"
                     /pseudo
     CDS             3563..3760
                     /locus_tag="JNZ05_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3804..3923
                     /locus_tag="JNZ05_003649"
                     /pseudo
     CDS             3804..3923
                     /locus_tag="JNZ05_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4173..5039
                     /gene="ospB"
                     /locus_tag="JNZ05_003650"
     CDS             4173..5039
                     /gene="ospB"
                     /locus_tag="JNZ05_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HAY8566708.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5368..6108
                     /locus_tag="JNZ05_003651"
     CDS             5368..6108
                     /locus_tag="JNZ05_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY8566709.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(6461..>6894)
                     /locus_tag="JNZ05_003652"
     CDS             complement(6461..>6894)
                     /locus_tag="JNZ05_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HAY8566710.1"
                     /translation="KLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGDKKADI
                     ALYLLSKFNFTKQDIAEMEKMNNNIYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVN
                     KKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAVSGKRFER"
BASE COUNT         2163 a         1289 c         1335 g         2107 t
ORIGIN      
        1 aactctagga taaatatttc tcactatata ttcagtattt gcagtattta ttggaaatac
       61 gttcttaata ttcttgatgg gactaatcat tttaatctct atactcaaat ataaacatta
      121 ccatgaacaa tataattgtt cctaagtgga atgtctccac ggagaacact ttatctatcg
      181 ggaaatagtg cgggcaacag tgtttaatta tatcgagtgc gattacaatc gctggcgacg
      241 gcaccgttgg tgtggcggcc tcagtccgga acaatttgaa aaccagaacc tcgcttaggc
      301 ctgtgtccat attacgtggg taggctcaca gagcctcact tacgactgcg tccatattat
      361 gtgggtagga tcaataaaga cgttttatat gtgtctgctt gcagccagaa aatttatacc
      421 ctgcaggtat cataaacatg cttgataact taattggggc tccctcttcc ttttatggtg
      481 taattgtttg attgaatact tccggggatt tcagtatgaa atgaagtata tttaatatac
      541 tttacaaaaa gtttgtatta gtggaataag gacaatagct tatccaaact gccaagcctc
      601 tttattataa gtaagatctg gcactggaat attctcaaaa ggactaacat catcaccacg
      661 accttcaacc aaccatttta ttgcatttaa atcccagaat agttcagtag tggtcaattt
      721 gggaaacgca caatattgta cggttacgtt atcgcaatag agtgaactcc cgccctatac
      781 ggcgttttca aggattagtc cttaatcgga caaccacatt aaagcataat aaataattaa
      841 gctttaataa tatactatag atacagttct attataaaaa atactatttt aggtgatgca
      901 ttaaattagt agaatattta ttcttttata aaagcaccaa tgaattgtcc aagtaaacat
      961 gaggtaaaat gtgtagagga taactgagtt tggtcacttt aactgtatta gtcgcagtct
     1021 gactctcgac tttaaaagga tgggaattac ctgtactgat agtgagaaag ctttccattg
     1081 aaataagtca aataaaagtt gatggcttca gtgctaaagt gaggtgcccg ctagccgtat
     1141 tcactcgaat cggaattcaa tgtattgctt ctgccatttc cataatattt cacgggaaaa
     1201 acaaaatctg aaaaactata cttaagtgtt gtattgagtc agttgagata caaatacata
     1261 tttatcagca cacgttacgt caaatacagg aggttgtcat gttggtactg aaaggcacgt
     1321 tggctattgc agccgtgatg acaatttatt gtcttatcgt tgttcttatg gaacgctttt
     1381 ctgattgatt tcatattggc gagagaacaa gaaggtgagt agaattgtcg cggaatcctg
     1441 aaactatcag cctcaggtat gaacaccaaa aggtaggtag agaaatctat ggcccccgtt
     1501 actttattcg cacaatgagg tattttttta tttttacaaa agttcaataa atattataat
     1561 aaaagatatg ttcttggtaa cataaacaaa tatttaatta aaataatgct tggcattgac
     1621 gtttatctta taaagtaaga agatcatgga catttcaacc aaaaaagtag agttctcgat
     1681 gaaatcaaaa ttccttgtac ttgcactttg tgtacctgcg atctttacta cacatgctac
     1741 cactaactat ccacttttca taccggacaa catcagtacg gatattagtc taggatctct
     1801 gagtggcaaa acaaaagaac gcgtttatca tcccaaggaa ggggggagaa aaattagtca
     1861 actggactgg aaatacagta atgcgactat tgttagaggt ggcatcgatt ggaagctaat
     1921 tccaaaagtg tctttcggag tttccggttg gactacttta ggtaaccaga aagcaagcat
     1981 ggttgataaa gactggaaca attccaatac ccctcaggta tggaccgacc agagctggca
     2041 tcccaatacg catctccgtg atgctaacga attcgagctg aatcttaaag gttggttatt
     2101 aaataatttg gattatcgac ttggactaat agcaggttac caagagagtc gttacagttt
     2161 taatgcaatg ggagggagtt atatttatag tgaaaacggg gggaacagga ataaaaaagg
     2221 ggcacatcct agtggtgaaa gaacaatagg ttacaaacag ctcttcaaaa taccttatat
     2281 tggattaact gctaattacc gccatgagaa ttttgagttt ggagcagaac tgaaatatag
     2341 tggttgggtt ctttcatctg atacagataa acactatcag actgagacaa tttttaaaga
     2401 tgaaataaaa aaccaaaatt actgctctgt tgctgcgaat attggatact atgtcacccc
     2461 cagtgcaaaa ttttatatag aaggctccag aaattacatt tctaataaaa aaggtgatac
     2521 atctctttat gagcaaagta ccaatatatc tggcaccatt aaaaatagtg caagtattga
     2581 atatattggt tttctcactt ccgcaggtat aaagtatatt ttttgaattg gttcaccgag
     2641 ttgattacgt tttcatggct aaaaattgcc gcccattgtg agtcatattc ggcctgactt
     2701 tccagaacca tacgaactgc ccgttgacgg acttcggggg aaaaacgtgt atttttagtc
     2761 attctgttta cctctttctc agggcgttta gtctccagga tttccggggc ggttcaaaaa
     2821 actcttcgtt gattactgtg gacttactgt tggcattacc gaccctgaga ccggagaaat
     2881 aagaaccgct tagatcatcg tggctgttct cggggtatca agttacacat gggcagaggc
     2941 tacccaaggt ctactattat cgcctgaaag cgctgatgga acagtgctat cagggacatg
     3001 ctgatgaaaa tacctaaata atcagccagc actctatctt tccaaatcca cagcatagca
     3061 aagagagcaa aagagcctgt aaattcagaa attaaggaaa cgcctcttgg tgcacataat
     3121 ctatcttatg ttaaatgaaa tctaatttct tgattatatg ctggatcgtt cgtgggtggc
     3181 tttgccccta tatttccaga cacctgttat cattggcccg ctgccgcaga tattcccgtg
     3241 gcgagcgata atccagcgca ctatgcggat gccattcgtt ataatgctcg aatgcctctg
     3301 caaggttctt tgctgccgtt aacccgtcag gtttgggcat gatactgatg tagtcacact
     3361 ttattgtttt cacgaagctc tttgctattc tgttactctc cggactccgc accgccgtgt
     3421 tcttcggttc aacaaagtcc caacatccgg gtgaactggc gtgtttcatt agcccggtag
     3481 catgaaccat tatccgtcag tcactccact ggagacgacg gaagatcgtt gccgaagcgg
     3541 tgttccaccg ctcccagcat gtcgccaatg ggagagcatc tacggtctga ttgaactgtt
     3601 tattcaaatt gtccaccgtc tgtcagtacg ctctgaacgt cggctggtta aaacactgct
     3661 cgctgatttt cagagggttc acggtaaaac agcactttta ttccggattg cggaagcggc
     3721 tctgaataat ccggatgggc tggttaaaga ggttgtgtac catctgcata ttgttgaacc
     3781 agatcgcagc ggtaagagaa gttcatcata cttggcgcag ttacgcgatg tttctgctag
     3841 aggtgatgcc gtaaagaacg gcagaacatt accggaacaa gatagcggtc tacctgcact
     3901 ggtatcagac ccggggcttc cccggatgat ctccacggta ctttaggtac tgtgtctata
     3961 ttatgtaggt aggatcaatt aactttgtag ttgaaattct ttcattgaaa taatgaataa
     4021 aaaagacaaa ccgcattaaa caatgccaga atataattgc atttttgttc cgttttttta
     4081 aatcatagta tacaaagaag cttgcatact atatgaaaat ataacaccct ttactatgat
     4141 gtttataaaa caatatatgg agtcatgtag gtatgaattt agatggtgtt agaccatact
     4201 gtagaatagt caataaaaag aatgaaagca tatcagatat tgcatttgca catataataa
     4261 aaagggtaaa aaattcatca tgtactcacc caaaagcagc attggttttt ttaggagaga
     4321 aaggtttttg tgatagcaat gatgttctat ctattatggg acaacaaata ccaagagtat
     4381 ttaagaacaa gatgttatat gattatgttt ttaaaaatga aaaaagtaaa aatgattttc
     4441 taaaaatggc tgaatcatgg ctaccacaga gtgaaccaat agtaataaat aatgatgatg
     4501 acgcattgaa tgctgctgct tatttttctg taaaaaaagc gaaaataaaa acagtaaacg
     4561 atactgattt taaagagtat aataaggttt atattcttgg gcacggtagt cctggttctc
     4621 atcaattagg ccttggttcg gaacttattg atgtacaaac aatcatttca agaatgaaag
     4681 actgtggtat tctaaatgtg aaagatatcc gttttacttc atgcggctcc gctgataaag
     4741 tggctcctaa aaattttaac aatgcccctg ctgaaagtct ttcttgtatc cttaactctc
     4801 tgcctttttt taaggaaaaa gaatctttgc tagagcagat aaaaaaacac cttgaaaacg
     4861 atgagtcatt gagtgatggt ctaaaaatat ccggctatca tggatatgga gttcactatg
     4921 gtcaagagct ttttccctac tcacattatc gttcaacttc aattcctgct gatccggagc
     4981 atacagtaaa aagaagctct cagaaaaaga cttttattat taataaagaa ctggattagt
     5041 ataaaatttt taacctatag ttaattgttt gcatttaaga tgaagttatt ttatctttag
     5101 tagaaaagtg cttccctatg agggaaaatc acaaatcatc ataatacaga gacaaaacga
     5161 tacgaacaaa tagataaaaa acatcattct tttaccacat tatttttcgt gaatatgaaa
     5221 aataatgtta ttactttaat ataagactat tttttgtttt tccatcactc tgttcaaatt
     5281 tttccgcatg acttgtgttt tttgtaacac agctcgtttt ttacagctga ccaaaatcat
     5341 caattaatta tgctaaggaa ataaattatg aaaaccaaaa actttcttct tttttgtatt
     5401 gctacaaata tgatttttat cccctcagca aatgctctga aggcagaagg ttttctcact
     5461 caacaaactt caccagacag tttgtcaata cttccgccgc ctccggcaga ggattcagta
     5521 gtatttcagg ctgacaaagc tcattatgaa ttcggccgct cgctccggga tgctaatcgt
     5581 gtacgtctcg ctagcgaaga tgcatactac gagaattttg gtcttgcatt ttcagatgct
     5641 tatggcatgg atatttcaag ggaaaatacc ccaatcttat atcagttgtt aacacaagta
     5701 ctacaggata gccatgatta cgccgtgcgt aacgccaaag aatattataa aagagttcgt
     5761 ccattcgtta tttataaaga cgcaacctgt acacctgata aagatgagaa aatggctatc
     5821 actggctctt atccctctgg tcatgcatcc tttggttggg cagtagcact gatacttgcg
     5881 gagattaatc ctcaacgtaa agcggaaata cttcgacgtg gatatgagtt tggagaaagt
     5941 cgggtcatct gcggtgcgca ttggcaaagc gatgtagagg ctgggcgttt aatgggagca
     6001 tcggttgttg cagtacttca taatacacct gaatttacca aaagccttag cgaagccaaa
     6061 aaagagtttg aagaattaaa tactcctacc aatgaactga ccccataaag ctggacagcc
     6121 tgtatcaggc tatggagggc ccatagacaa atctacccta tatgagcata ggaggagtct
     6181 atgggcacac cacgttttac ccctgaattt aagggattac tggaaaggct gggacatatc
     6241 cctccggcag aagcagaaaa agcttattat gctgccatcg gaaacgatga tctggcaacc
     6301 tgagttcaca gataaaacat tctctaggaa actcggggcg gttccgttca ccacatgcaa
     6361 tgtggtgttg caggggaacg gtctgcacca tcccctatat cgatcaatat aacaaaaatg
     6421 acaacttcag attcagggca caacctaaat atattttagg tcacctctca aatcgtttgc
     6481 ctgataccgc tccatttttt aataaaaaat caatcatttt tgaatctttg cttttcattg
     6541 cattgtcaag catagtgtcc ccactatttg ctttttgaaa ttttttgttt acatcaacca
     6601 atccattatt gataaaatat tctagcactt tatagttggc accatcttta ctaagtaagt
     6661 attcaacatc atacagatta caatatatat tgttgttcat tttctccatc tctgcgatat
     6721 cttgttttgt aaaattaaat ttagatagta aataaagtgc tatatcagct tttttatcgc
     6781 ctaaagaata agcaagggcc tgatgagcca tttttttatc agtcacttta ttgttaatct
     6841 cttcataatt agatgcttta atcgcatcct ccaaagatat gtctctgagt ttta
//
LOCUS       DADMEQ010000162         6854 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.164, whole genome shotgun sequence.
ACCESSION   DADMEQ010000162 DADMEQ010000000
VERSION     DADMEQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.164"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            239..2671
                     /locus_tag="JNZ05_003653"
     CDS             239..2671
                     /locus_tag="JNZ05_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAY8566711.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGLFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEALI
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2702..4207
                     /locus_tag="JNZ05_003654"
     CDS             2702..4207
                     /locus_tag="JNZ05_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HAY8566712.1"
                     /translation="MLQKALFFMEYNMSISSVIKSLQDIMRKDAGVDGDAQRLGQLSW
                     LLFLKIFDAQEEALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPAL
                     KNLTAPIDKNPRGYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIY
                     EQILKDLQSAGNAGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKN
                     KYVKSVADHQTLQQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWD
                     EQLDVIVTNPPFGGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDG
                     TLFGEGVKTKIKKLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYE
                     HPYPAGVKNYSKTKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFN
                     LDIKNPHQAETVSHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4207..5868
                     /locus_tag="JNZ05_003655"
     CDS             4207..5868
                     /locus_tag="JNZ05_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAY8566713.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6089..6430
                     /gene="symE"
                     /locus_tag="JNZ05_003656"
     CDS             6089..6430
                     /gene="symE"
                     /locus_tag="JNZ05_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HAY8566714.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6477..6797)
                     /locus_tag="JNZ05_003657"
     CDS             complement(6477..6797)
                     /locus_tag="JNZ05_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566715.1"
                     /translation="MLFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRL
                     RLSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQME
                     SALV"
BASE COUNT         2024 a         1624 c         1566 g         1640 t
ORIGIN      
        1 ttcttgttcg aaagaaacgc aactataaga tttttatata ttttgttaga gttgatatgg
       61 ttcctgtaac ttctgtttat ctgtctctga tatgcccaga ttaattcaag caagatataa
      121 cagagatatc aatgtgatct ctgctacata ctttacgttg aaacaccgcc ctaaatcata
      181 gccaggctat tgattttatg tacaatcaga atcgtcctga taaaagcgag aacaccccat
      241 ggcggaactg aacctaagta acctgacgga agcagatatc attaccaaat gcgttatgcc
      301 agccattctc aatgcaggct gggacaacac aacgcaaatc agacaggagg tcaaactccg
      361 ggacggtaaa gtcatcgtac gtggtaaagt tgcggcacgc agaacggtaa aatctgctga
      421 catcgtgctg taccacaagc caggcattcc cttagccgtg attgaagcaa aagccaacaa
      481 acatgaaatt ggcaaaggga tgcaacaggg cattgaatat gcgcgcctgc tggacgttcc
      541 ttttgttttt gccaccaacg gtgatggctt tatcttccgc gatgccaccg cagccgaagg
      601 tgaatgcctt gaaaagcaaa taacgctgga tgacttcccc tcccccgctg aactctggca
      661 aaaattctgc ctctggaaag gatacacaca agctcagctt ccggtgatta ctcaggacta
      721 ttacgacgat ggcagcggca aatcgcctcg ttattatcaa cttcaggcaa tcaacaaaac
      781 cattgaagcc gtctccaacg ggcaaaaccg cgttctgctg gtcatggcga ccggaacagg
      841 gaaaacctat accgcattcc agatcatctg gcgcctgtgg aaatcaaaaa ataaaaaacg
      901 cattttgttc cttgccgatc gcaacattct ggtcgaccaa accaaaaata atgatttcca
      961 gccatttggt acggcaatga ccaaagtcag cggacgcacc attgatcccg cttatgaaat
     1021 tcacctcgcg ctctatcagg ctataactgg cccggaggaa gaccaaaaag cgtttaaaca
     1081 agtcgcacca gatttcttcg atctgatcgt gatcgacgaa tgccatcgcg gcagcgcatc
     1141 cgaagacagc gcctggcgag agatccttga ttatttcagt tccgccaccc aaattggctt
     1201 aaccgccacg ccaaaagaga cgcatgaagt ctccagcacg gattacttcg gcgatccggt
     1261 ttacgtctac tcactaaaag aagggatcga agacggcttc ctcgctcctt ataaagttgt
     1321 ccgtgttgat attgatgtcg acctgcaagg ctggcgccca accaaagggc aaactgactt
     1381 aaacggcgaa gtgattgacg atcgtatcta taaccagaaa gatttcgatc gcacgatggt
     1441 gatcgacgaa cgcacagaac tggttgccag aaccattacc gactacctca agcgcaccaa
     1501 tccgatggat aaaaccatcg tcttctgtaa cgacatcgat catgcagaac gtatgcgccg
     1561 cgccctggtt aatctcaacc cgcagcaggt gaaaaagaac gacaaatacg tcatgaaaat
     1621 caccggcgat gatgaaattg gcaaagctca gttggataac ttcatcaacc cgaaaaaacc
     1681 gtacccggtt atcgcgacca cttcagagct gatgaccact ggtgtggatg ctaaaacctg
     1741 caaactggta gtactagacc agaacatcca gtcgatgacc aaattcaagc aaattatcgg
     1801 tcgtggtaca cgcatcgacg aacgttacgg caaactctgg tttaccatcc tcgactttaa
     1861 aaaagccacc gaactgtttg ccgatgagcg tttcgatggc attcccgaaa aagtcatgga
     1921 taccacacca gaggatatcg ccgatccaga atctgatttt gaagagaaac tcgaagaaat
     1981 cagcgaacat gacgacgaac aggtaacagg cgttgatgaa ccgcctgcgc caccatacca
     2041 ggttaccgat accgatgatg tcggcctatt tccggaagaa gacgagaaga aaatccgcaa
     2101 gtttcacgtc aacggtgtag cggtgggcgt tattgcacag cgcgttcagt attacgacgc
     2161 cgacggtaaa ctggttaccg aatcctttaa agattacacc cgcaaaacac tgctcaaaga
     2221 atatgcctcg ctggatgact ttacccgcaa gtggcaggac gccgatcgca aagaagcgct
     2281 cattcacgag ctggagcaac aggggatcat ctgggaagta ctggcagaag aagtcggtaa
     2341 agatctcgac ccgttcgaca tgctttgcca cgtagtgtat ggtcagccgc cgttaacccg
     2401 caaagagcgc gccgagaacg tgcacaagcg gaactacttc acaaaatact ctgaagcagc
     2461 gcaagccgtg ctcgataatc tgctggataa atacgccgat gcgggcgtac aggagatcga
     2521 aagtattcag gtgctgaaac ttaaaccatt cgacagcatg ggcaccttac cggagattat
     2581 taaaaccgga tttggcgacc gtaacgggta taatcaggcg ctcagcgagc tggaaaacga
     2641 aatctaccaa ttaccgcccc gctctgctta acaactctct tttgcgcctt ctgcaaccat
     2701 catgttgcag aaggccttgt ttttcatgga atacaacatg tctatcagct cagtaatcaa
     2761 atcattacaa gatattatgc gcaaagatgc cggtgtggac ggcgatgcgc agcgtctcgg
     2821 tcagctctcc tggctgctgt ttttgaaaat cttcgatgcc caggaagagg cgctggaact
     2881 ggagcaggat aactatcaat atccgatccc acagcgttat ttgtggcgca gttgggccgc
     2941 caacgcgcag ggcattaccg gtgattccct gctggaattc gttaatgatg atctgtttcc
     3001 ggcgttgaaa aacctcactg cgcctatcga taaaaaccca cgcggctacg tggtgaagca
     3061 ggcgttcagc gatgcctata actatatgaa aaacggtacg ctactgcgcc aggtgatcaa
     3121 caagctgaac gaaattgact ttaccagcgc cagcgaacgc catctgtttg gtgatattta
     3181 cgaacagatc cttaaagatc tgcaatctgc gggcaatgcg ggcgaattct atactccacg
     3241 cgccgtcact cgctttatgg tggatcgcgt tgatccgaaa ctcggcgaat ccattatgga
     3301 cccggcctgc ggtacgggcg gttttcttgc ctgcgcgttc gatcatgtaa agaacaaata
     3361 cgtgaagagc gtcgccgatc atcagacact gcaacaacag atccacggtg ttgagaaaaa
     3421 acagcttccg cacctgctgg cgaccaccaa tatgctgctg cacggcattg aagtaccggt
     3481 acaaattcgt cacgacaaca ccctgaataa accgctttcc tcctgggatg agcaactgga
     3541 tgtcattgtt accaacccgc cgtttggtgg cacggaagaa gacggtattg agaagaactt
     3601 tccggcagag atgcaaaccc gcgaaacggc ggatctgttc ctgcaactga ttgtggaagt
     3661 acttgcgaaa aacggtcgtg cggcggtggt attgccggat ggcacactat ttggcgaagg
     3721 tgttaaaacc aaaatcaaaa agctacttac cgaagagtgc aacctgcata ccatcgtgcg
     3781 tttaccgaat ggtgtgttta atccctatac cggcattaaa accaacctgt tgttctttac
     3841 caaaggtcag ccaaccaaag agatttggtt ctatgaacat ccgtatccgg cgggcgtgaa
     3901 aaactacagc aaaaccaagc cgatgaagtt tgaagagttt caggcggaga tcgactggtg
     3961 gggtaacgag gccgatggtt ttgccagccg cgtcgagaat gagcaagcgt ggaaagtcag
     4021 cattgatgac gtgattgcac gcaacttcaa tctggatatt aaaaacccac atcaggcgga
     4081 aaccgtcagc catgatccgg acgaactgtt agcgcagtat gcaaaacagc aggcggagat
     4141 ccagacgctg cgtaatcaac tgcgcgatat tcttggcgct gcgctgtctg gcaaggaggt
     4201 taactaatga gtgtggaaaa gctgatcgtt gatcatatgg aaacctggac ctcggcgttg
     4261 caaacccgtt ccaccgccgg gcgcggcagc tccggtaaga ttgatttgta tggtattaag
     4321 aaattgcgtg agctgattct ggaactggcg gtgcgcggta aactggtgcc gcaggatccg
     4381 aatgatgaac cggcgtcgga cctgctgaag cgtattgcgg cggaaaaagc agagctggtg
     4441 aagcaaggca aaattaaaaa gcaaaaacca ctgccagaaa ttagtgagga agagaagccg
     4501 tttgaattgc cggagggatg ggagtgggtg cacctccctg atatttattg ctcaatttct
     4561 gagtcatcta gaaaaataaa atcctccgaa atactacctg agggaaaata tcctgttatc
     4621 gagcaaagcc aagaatttat ttctggttat tgcaacaatg aatgtctgtt aataaagtta
     4681 aataatccag ttatagtttt tggtgatcac acaaggaata ttaaatttat tgattttgat
     4741 tttgttgtcg gcgctgacgg tgtgaaaatt ttatcaccta tattaatttg tgagcgtttc
     4801 tttttttggc agttacgatc atttaaatta gatgtaagag gatacgcgag acattttaaa
     4861 gtattaaata gctgcttatt tgcattaccc cccattgcag aacaagagcg aatagttgaa
     4921 aaagttagct ctttgatgtc cctctgcgac caactagaac agcaatccct gaccagtctg
     4981 gacgcacatc agcaactggt tgaaaccctg ttgggaacgc ttacagacag ccaaaacacc
     5041 gcggaactgg ctgaaaactg ggcgcgtatt agcgagcatt tcgacaccct atttaccacc
     5101 gaagccagcg tggatgcgtt aaaacagacc attctgcaac tggccgtaat gggcaaactt
     5161 gtgccgcagg atccgaatga cgaacccgcc tctgaactgc tcaaacgtat tgcgcaggaa
     5221 aaagctcaat tggtgaaaga agggaaaata aaaaaacaaa aaccactgcc gccaattagc
     5281 gatgaggaaa aaccgtttga acttccggaa gggtgggaat ggtgtcgtat tggtaatata
     5341 gttaatatta aatctgaatt agtatcacca aaagattatt taaatttata tcaagtagca
     5401 cctgatatta ttgaaaaggg cactgggaga gtgatatcaa agagaacagt gaaagaatca
     5461 ggagtcaaag ggccaaatag tcgcttttat aaaggacaaa ttgtttattc taaaatccgc
     5521 ccgtcattat ccaaagtttt tttagcagag tataatggtc tttgcagtgc tgatatgtat
     5581 ccattagact gttacattaa tccaaattac ttattgaaat atatcctttc cattccattt
     5641 cttatgcaag tcaaaaaagc agagaacaga ataaaaatgc cgaaacttaa ttctgacagt
     5701 ttttacaata ttattgttgc gattcccccc tataatgaac aacaagcaat atttgataag
     5761 ataaattcta ttgaagcagt atgcaatggt cttatttctt atattgggat ttatcataaa
     5821 acccaactgc atcttgcaga cgcactcact gacgcagcga taaactaatt ccctcgccct
     5881 cacaaaacca ataaagcggc taaatagccg ctttattcac atcaccaaaa tttatatttc
     5941 acgctaattt tcatcttatt ttcattaatt tttagctgga ctttccctgt atttactgat
     6001 gatatatcca ggtatttagc acggtgcgga tgtgcgccaa cacactcgca ccgctaatca
     6061 caatcactat ttatagagaa tagtaatcat gactgacacg cattctattg cacaaccgtt
     6121 agaagcagaa gtctccccgg caaataatcg tcaattaacc gtgagttatg cgagtcgtta
     6181 cccggattac agccgtattc ccgccatcac cctgaaaggt cagtggctgg aagccgccgg
     6241 ttttgccacc ggcacggcgg tagatgtcaa agtgatggaa ggctgtattg tcctcaccgc
     6301 ccaacccgcc gccgccgaag agagcgaact gatgcagtca ctacgcaagg tgtgtaagct
     6361 gtcggcgcgt aaacaaaggc aggtacagga atttattggg gtaattacag gtaaacagaa
     6421 agtcgcctga tacattgacc cggtagcgcc agtgctaccg ggtattattc gccgcgttat
     6481 accaacgcgc tctccatctg tttttcaaat gcctgacgaa ttaatcccat aatgtaagga
     6541 agttgtgcga ggtcgctgaa accaatttct acatcgccat taccccagcg cccaacattt
     6601 gtcacatctt tggcaatacc tttcggatcg accagttcat gaaactgcat attaagcgac
     6661 aatcgcagtc ggcttttttg cggcacaaca tcaacaaaat tcgtttccgc tttaaacgca
     6721 atatatagct tcaatacttc ctgcgtgatc cctgcgtcca ggcgcataac ttcatcgcga
     6781 agatgatcga ataacactga ggtagcctga gtttaacgga cgctccttcc tgaaatagaa
     6841 tggcatcaga agga
//
LOCUS       DADMEQ010000163         6796 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.165, whole genome shotgun sequence.
ACCESSION   DADMEQ010000163 DADMEQ010000000
VERSION     DADMEQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.165"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            337..579
                     /locus_tag="JNZ05_003658"
     CDS             337..579
                     /locus_tag="JNZ05_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566716.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAVTGGGDGSNTGTTTTTTTSTQ"
     gene            693..1331
                     /locus_tag="JNZ05_003659"
     CDS             693..1331
                     /locus_tag="JNZ05_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAY8566717.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQSTLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTIIKAAKQ"
     gene            1328..2081
                     /locus_tag="JNZ05_003660"
                     /pseudo
     CDS             1328..2081
                     /locus_tag="JNZ05_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
     gene            2081..4177
                     /locus_tag="JNZ05_003661"
     CDS             2081..4177
                     /locus_tag="JNZ05_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAY8566718.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ERMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4224..4502)
                     /locus_tag="JNZ05_003662"
     CDS             complement(4224..4502)
                     /locus_tag="JNZ05_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566719.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4503..4646
                     /locus_tag="JNZ05_003663"
     CDS             4503..4646
                     /locus_tag="JNZ05_003663"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566720.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4772..5182
                     /gene="psiE"
                     /locus_tag="JNZ05_003664"
     CDS             4772..5182
                     /gene="psiE"
                     /locus_tag="JNZ05_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HAY8566721.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5226..6700)
                     /gene="xylE"
                     /locus_tag="JNZ05_003665"
                     /pseudo
     CDS             complement(5226..6700)
                     /gene="xylE"
                     /locus_tag="JNZ05_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
BASE COUNT         1703 a         1702 c         1788 g         1603 t
ORIGIN      
        1 aatgccgaca ataagtcggc attgttttat cagataaatc cccttgtctg tgatttaacg
       61 gaaatcatac cgtgaggtta atcctaaaat agatttttaa tcgttgttta tttcggagaa
      121 tacgcagatt aattactttt gtttttattt taagtttatg atttttattg ttatttaaat
      181 ataagttgaa acttatattt gatattcatt ccaattatcc taaaactcca tcgctaattc
      241 ctcgcgccgt aattcgcatg ctttagttgt gtatactcga tcccgcccga aatgtttttg
      301 ggtaaatctc cattcattca atgaagggaa attgttatga aaaaagttct gtatggcatt
      361 tttgccatat ctgcgcttgc ggcgacttct gcgtgggctg cacctgtaca ggtgggcgaa
      421 gcggcagagt cggcagcaac gtcggtttcg gcggggagtt cctccgcgac cagcgtcagc
      481 accgtaagct cggcggtggg tgtcgcgctc gcggtaaccg gtggcggtga tggttctaat
      541 accgggacca ccacaaccac gaccaccagt acccagtaat aaagtatgta tccccaaaat
      601 aatttgagtc attgcatctg tggcttgaag tatgaaggga tttaccataa ccacactccg
      661 gtgtggttat tctgcccctc tggagaagag tcgtgaagcg acctgcactc attcttatct
      721 gcctgctatt acaggcctgt tcagccacga ctaaagagct gggcaattca ctgtgggaca
      781 gtctgttcgg cacgccaggc gtacagctga cggacgatga tattcaaaat atgccctacg
      841 ccagccagta catgcagctt aatggcgggc cgcaattgtt tgtggtgctg gccttcgctg
      901 aagacggaca acaaaaatgg gtcactcagg atcaatccac tcttgtcaca caacatggtc
      961 gtctggtgaa gactttgctt ggcggcgaca acctgattga agtgaataac cttgccgccg
     1021 acccgctgat taaacccgca caaattgttg atggtgcaac atggacgcgc acgatgggct
     1081 ggaccgagta ccagcaggta cgctacgcca ccgcacgctc agtcttcaaa tgggatggca
     1141 ccgataccgt caaggtcggc agcgatgaaa ccctggttcg cgtgctggac gaagaagtct
     1201 ccaccgacca ggcgcgctgg cataaccgct actggatcga cagcgaaggg caaattcgcc
     1261 agtcggaaca gtatctcggc gcggattatt tcccggtgaa aaccactatc atcaaggcgg
     1321 caaaacaatg attaaacaaa ctattgtcgc gttgcttttg agtgtgggag cgtcatcggt
     1381 cttcgcggca ggaaccgtca aggtgttcag caatggcagc agtgaggcca aaacgctaac
     1441 gggcgcagag catttaatcg atctggtagg gcaacctcga ctggcaaaca gctggtggcc
     1501 tggtgcggtg attagcgaag agctggcaac ggcggcagca ttgcgtcagc agcaggcgtt
     1561 gctgacacgg ctggcagagc agggcgcaga ttccagcacc gacgatgccg ccgccattaa
     1621 cgccttacgc cagcaaatcc aggcattgaa agtgacaggc aggcaaaaaa tcaatcttga
     1681 tcccgatatc gtccgcgttg ccgaacgcgg taacccgccg ttgcagggca actacacgct
     1741 gtgggtcgga ccaccgccgt ccacggtcac gttgttcggg cttatcagcc atcctggcaa
     1801 tcagccatcc tggcaatcag ccattcactc caggtcgcga cgtggcgagc tatctctctg
     1861 accagagcct gctcagcggt gcggatcgca gctacgcgtg ggtcgtttac ccggacggac
     1921 gcacgcaaaa agcgccggtg gcttactgga acaagcgtca cgtagagccg atgcccggca
     1981 gcattattta tgttggcctc gcagattccg tctggagtga gacgcctgat gcccttaacg
     2041 ccgacattct tcagactctg acgcagcgga taccccaata atgaaaaaaa gacatctgct
     2101 tagcttactg gcgctgggca ttagcacagc ttgctacggc gaaacatatc ctgcgcccat
     2161 tggtccgtcg cagtcggatt tcggtggcgt aggattatta caaactccca ccgagcgtat
     2221 ggcacgggaa ggggagttga gtctgaacta tcgcgataac gatcagtacc gttattactc
     2281 agcttcagtg caactcttcc cgtggctgga aacaacgctg cgctacaccg acgtgcgcac
     2341 ccggcagtac agcagcgtcg aagcgttctc tggcgatcaa acgtataaag ataaagcctt
     2401 cgatctcaaa ctgcgtttgt gggaagagag ttactggctg ccgcaagtgg cggttggtgc
     2461 gcgggatatc ggcggtacgg ggctgtttga tgcggaatat cttgttgcca gcaaagcctg
     2521 ggggccgttc gattttacgc tcggcctggg ctggggatat ctgggcacca gcggtaatgt
     2581 aaaaaatccg ctctgttcag ccagtgataa atattgctat cgcgataaca gctacaaaca
     2641 ggcgggatct atcgacggta gccagatgtt ccacggtcct gcctcactgt ttggcggcgt
     2701 ggaataccag acgccctggc aaccgctgcg tctgaaactg gagtatgaag gtaataatta
     2761 tcagcaggat tttgccggga agctggagca aaaaagtaag tttaacgtcg gggcgattta
     2821 tcgcgttacc gattgggccg acgttaacct tagctatgaa cgtggcaaca cctttatgtt
     2881 tggcgtcacg ctgcgcacca actttaacga tctgcgcccg tcctacatcg ataacgcccg
     2941 cccgcaatat caaccgcagc cgcaggatgc cattttgcag cattcggtgg tggcgaatca
     3001 gttaacgttg ctgaaataca acgccggact tgccgatccg cagatccagg cgaaaggcga
     3061 tacgctgtat gttaccggcg agcaggtgaa atatcgtgat tcgcgcgaag ggatcatccg
     3121 tgctaatcgg atcgtgatga acgatctgcc ggatgggatc aaaacgatcc gcattacgga
     3181 aaatcgcctt aacatgccgc aagtgacgac ggaaaccgat gtcgccagcc tgaaaaatca
     3241 tctcgccgga gagccgttgg gccacgaaac gacgctggcg cacaaacgcg tcgagccagt
     3301 ggttccgaag tccaccgagc agggctggta tatcgacaaa tcacgctttg atttccatat
     3361 cgatccggtg ctgaaccagt cggtcggtgg cccggaaaac ttttacatgt atcagctggg
     3421 cgtgatggga acggcagatt tgtggctgac ggaccatcta ctgaccactg gcagcctgtt
     3481 tgcaaatctt gccaacaact acgacaagtt taactacacc aatccaccgc aggactcgca
     3541 cttaccgcgc gttcgtaccc atgtgcgcga gtatgtgcag aacgatgtct atgtgaataa
     3601 cctgcaagcc aactacttcc agcatctggg caacggcttc tacggtcagg tctatggagg
     3661 ttatctcgaa accatgtttg gcggtgcggg ggccgaagtg ttgtatcgtc cgctggacag
     3721 taactgggcg tttggtctgg atgccaacta cgttaaacag cgcgactggc gtagtgcaaa
     3781 agatatgatg aaattcaccg actacagcgt gaaaaccgga catctgaccg cctactggac
     3841 gccatctttc gcccaggacg tgttggtgaa agccagcgtc gggcagtatc tggcagggga
     3901 taaaggcggc acgctggaga tcgctaaacg ctttgacagc ggcgtggtgg tgggtggcta
     3961 tgccacgatc accaacgttt cgaaagagga gtacggcgaa ggggacttca ccaaaggcgt
     4021 gtatgtctcg gtaccgttgg atctcttctc gtctggcccg acacgcagcc gtgcggcgat
     4081 tggctggacg ccgctgacgc gtgacggtgg tcagcaactt gggcgtaagt tccagttgta
     4141 tgacatgacc agcgaccgta gcgtaaattt ccgctaagtc atgggaaagg tgccagtttt
     4201 cgcactcact ggcaccctca ttcttaatga cctgttgagc catcaggaac gcagttcacc
     4261 cccggtttac gatctggtgt gtaaaggcga aaacattctg tcttcctgtc cgtcgatatc
     4321 ggttgcaggt ctggttgcct gagcgactgg gccatcattg ggctggagac attgctttgc
     4381 ggtaggtcgg ctttatcagc agtaccgagc ggaccagcat aagcaggaag aacagagact
     4441 gataacatca aagcagcaaa ataaggcttc atttttacca cctttatcag gttacgtttc
     4501 atttgttcca gaggaacatt gtcgattttt cgcgcattgc tggtggctgg gaatcacctg
     4561 aatgggtgat ttttgaatta ccggctttgg tgcggtttgt cttgccggat gcgccgccag
     4621 gcgcggctta tccggcctac gggtaggtat atccggcttt ggtggaggcg cgctccaaat
     4681 ccaggttgaa caaaacatac acaaaaaata tagatctccg tcacattttt gcgttataca
     4741 ggaagctcgc cactgtgaag gaggtactgc tatgacgtca ctctctcgtc cgcgcgtgga
     4801 gtttatctcc accattttgc agaccgtact caatcttggc ctgctgtgcc tcggcctgat
     4861 tttggttgtc ttcctcggca aagaaacggt gcatctggct gatgtgctgt tcgcgccaga
     4921 acaaaccagc aaatatgagc tggtagaagg actggtggtt tactttctct atttcgaatt
     4981 tatcgcgctg attgtgaagt actttcagtc cggttttcac ttcccgctac gttactttgt
     5041 ctatattggg atcaccgcca ttgtgcgctt gatcatcgtc gatcataaat cgccactgga
     5101 tgtgttgatc tactcggctg cgatcctgtt actggtgatc accctgtggc tgtgtaattc
     5161 gaaacggctg aagcgggagt aaaaagacag cacgccgaaa tggcgcggcg tgctggacag
     5221 gaagattaca gcgtagcagt ttgttgtgtt ttcttcgttt ccggttccca gagcgcttcc
     5281 agctcctcaa gggttttacc tttggtttcc gggacaaatt tccacataaa cagtgctgcc
     5341 agaacgccca tacaaccgta aatccagtag gagaaaccgt tgtggaaatg ggccaccagc
     5401 caggagtttt tgtccatcat cgggaaggtc caggagacga agtagttcgc cagccactgg
     5461 gccgccaccg cgattgccag cgctttacca cgaatagcat tcgggaagat ttccgacagc
     5521 agtacccagc ataccggacc ccaggacatg gcaaaggcgg caacatagaa cagcatcgac
     5581 agtagcgcca caatacccga gcctgagtgt aaaacgcggt actgaggcta aacataccga
     5641 ttgccattcc gagtgcgccg ataatttgca gtggcttacg accaaattta tccacagtca
     5701 taattgccag tacggtaaag gtgaggttga taactccgac aataatggtc tgcaacagcg
     5761 cgatatccgt gctggccccc agcgttttga acacttccgg cgcgtagtac agcaccacat
     5821 tgatgccgac aaattgctgg aagatggaga gcataacgcc gattacaatc acgcccacgc
     5881 caaacatcag cagacgacca ccggttttgc ggccatgatc cagggagtgt ttaatttcct
     5941 gtactgcctg agttgcaagc gtgttgccca taattttgcg caggatactt tccgtctgtt
     6001 cttgcttgcc gcgtgacatc agccagcgag gactttctgg cacggtatac agcagcatta
     6061 agaacagcag tgcagggata cattccgagg caaacatata acgcctgccg tcagtattca
     6121 gccagctggc atcaccggaa cgggcaataa aatagtttac gcagtaaact aaaagttgcc
     6181 cgaaaataat cgcaaactgg ttaaatgaaa ccagttttcc gcgaatatga gctggagcca
     6241 gttccgcaat atacattggt gaaagcattg aggctaaacc aacgccaata ccgccaataa
     6301 tacgataaat aacaaattcc gggacataac ctgccagata aataggtact gtgttgtccg
     6361 ggtttataga ggtaaaacca agttctggcc aggcagaacc tacaccagaa ataaaaaaca
     6421 ggacagcaga aatcttaagt gaatcacgac ggccgaagcg gttactgcaa taaccaccga
     6481 gggcaccgcc gatgatgcaa ccaatcagag cgctggctac gcaaaatcct aacagggagt
     6541 tggcagcgga ttcacttaag ttttgtggag caacaaagac ggtattgagt gactcaacag
     6601 taccggaaat aacggcggtg tcgtagccaa ataataaacc acctaatgta gcgactaagg
     6661 taatcgaaaa tatataactg gaattatact gggtattcat tcagacctgc cttagaccat
     6721 tctgatcttt tatcagtgca ttgatgttgg aattgtcagg cagttaaggc aattatcatt
     6781 ttttacaatg cgttta
//
LOCUS       DADMEQ010000164         6781 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.166, whole genome shotgun sequence.
ACCESSION   DADMEQ010000164 DADMEQ010000000
VERSION     DADMEQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.166"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            1..3524
                     /locus_tag="JNZ05_003666"
                     /pseudo
     CDS             1..3524
                     /locus_tag="JNZ05_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3534..6749
                     /locus_tag="JNZ05_003667"
     CDS             3534..6749
                     /locus_tag="JNZ05_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HAY8566722.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSPEWQSAITQAINLIGEHKPSVPARTMAALAFIAQ
                     NDNQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVSL
                     ECPRTHFHSKEWLKVVATDPKAVRKLERYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAVVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPSYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSQMPEILVSPPWRGRKKKTSIRHFSLPD
                     LTLAPQTYRSPNKHWTPEQQTAIDHFSAMSFDERLATRGADIFLRELGFDVHSLEFNK
                     RLLNGQLDAFDAQQLQEYTSIGLLRTYHAHQAKSKQYREDATNALLAQDSIALMGGWR
                     TFQHGFYYGNEQRWGIWNLYLIAQMPREMAVSCWQQIVEADFHYTGVEYLLSVLGTDA
                     LPGLNTAFARHPKEIFPLLIHFGATELALPIARVWHLFAGQRNLARQWILQWPEHTAT
                     ALIPLVFTKSSDKSEAALLALRLLYEHGHGELLQTVANRWQRTDLWPALEQLLKQSPI
                     EIYPARIPKAPDFWHPQMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQL
                     KTFCQPQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTHILAWPQE
                     GKSARAVSGLNILTLMNNDMALMQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEE
                     LADRLVPTLGLNDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQ
                     LKAPEALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQ
                     RLIWGVIRQMNRVVYSTPFVWPQRGSSAMRKMSQLTCLRTL"
BASE COUNT         1797 a         1781 c         1634 g         1569 t
ORIGIN      
        1 tgtggagcaa gcgaaagata attctttgca accttcccaa acggtaacgg actctctcaa
       61 ggtagcggac ttatccacca ttattcagga acaaccctct ttcgtagcag aaacccgtgc
      121 ggctgacaaa aatacagatg ctgttttacc ttggctggcg aaagatattg ctgtcgtttt
      181 tccgcccgaa gttgtacaca ccacgttaag tcatcgccgc tttcccggag ttcctgttca
      241 gcaagcagac aaattgaccc aattacgtcg cttagcctgt agcgtgtcgc aacgggataa
      301 taaaacagcc acatttgact tcagcacctg ttctttagaa tggcaaaaca ccgtcgccca
      361 agcgatcagt cagatcgacg gcctgaaaac aacacagcta ccatcaccag taatggctgt
      421 actcacggca cttgaaatga aatgcacaag atataaagtg cgtgaggatg ttatggatca
      481 gatcgtccag gaaggcggtc tggaatatgc tactgatgta ataatacacc ttcaacagat
      541 tgatattaaa tgggattatg cgaataatgt cattattatt ctgccgtctg gcattgcacc
      601 tgactacttg gagcaatatt ccagatttga attacgccta cgtaaacatt tatcactggc
      661 ggaagagtct ctctggcaaa aatgtgcaca aaaacttatt gccgcaattc cacatattcc
      721 agaatggcgg caaccattaa ttgctttgtt attacccgaa aaaccagaaa ttgcacatga
      781 aattgcccag cgtctactgg ggcaaaaaaa attaccctcg cttgagtggt taaaaatagt
      841 ggcgactgat gagcacattc ttgcctcatt agaaaaatat cacgagccat atgccatttt
      901 tgatgattac tattgtggtg cgatatggtc agccaccgta ttacaggagc aaggtgttgc
      961 agccctgccc cgatttgctc cctatgccgc aagtgactac tgcgccgatg tgttgcgtca
     1021 tatcaatcat ccgttcgcat taacactgct tatacgtgta gccgggcata ctaaacgctg
     1081 tcacgatcga atgacgaaag cctgcgctgc gttcccacac gcagcactgg cagaacaggt
     1141 cattccctgg ctttcgacac ccgcagttgc agtgctgaaa tcatgccagc accaactgac
     1201 acagccctca aaccatgcca gcgccgatct actgccagcc atagtagtct ccccaccatg
     1261 gatttcgaaa aagaaaaaat cgccgattcc ggtgctggat ttagcgccat taggcatcga
     1321 gccaatctgt tatctgacag aagaaatcag taatcaactt ttggcgaaat atatctggta
     1381 ttcaaaacac atcacggtta gccatgaaga aagtactacc aacctgttgg caaggatggg
     1441 ttttcaacga cggatcgctg gtacatatat taaagctccc gaagcggtag ttgaggcatg
     1501 gctaaatgaa gattattcaa ccttactaag tgaatttaag gtgtttcatt cacctaccgg
     1561 gcattattgg cagttgggga ttttgacaac attgccgctg gaggctggag aaagcagtaa
     1621 aagcatggaa tgcccttact ctatctccac ataccgatac cgaatacgcc atgttacatt
     1681 ttggactcaa aggtttacct ggtttagtta actcaattac acgctatccg caggaagcct
     1741 tgcccatcac gaattacttc gcagcgagtg agctggctcc tgccgtcgcc cgtgccttca
     1801 acaaactcaa aacgctacgc gaaaacgccc gcacctggct gttgaaatac ccggaacatg
     1861 cccttaccgg cctgctgcct gcggcgctcg gcaaagccgg tgaagcacaa gataacgccc
     1921 gcgctgcctt gcgtatgctt accgaaaacg gtcatcagcc attactgcaa gaaatcgccc
     1981 ggcgttataa ccagtcggaa gtaacagacg cggtgaacgc tctgcttgcg ctcgatccct
     2041 tagataatca cccgacaaaa atccccactc ttccgacctt ttatcagcca tcgatctgga
     2101 cgcgcccggt attaaaagca aacgcccaat cactgccaga taacgccctc ctccacctcg
     2161 gcgaaatgct ccgcttccct caggaagagg ctctgtatcc gggattattg caggtgaaag
     2221 acgcctgtac cgctgactct ctggctgaat ttgcctggga tctgtttacc gcctggcaga
     2281 ccgctggcgc gccgtcgaaa gagagttggg cgttcactgc gttaggcgtt ctcggtaacg
     2341 atgacaccgc ccgcaaactg acgccattaa tacgcgcctg gcctggtgaa tcccagcata
     2401 aacgcgccac cgttgggttg gatattctcg ccgctatcgg tagtgatatc gccctgatgc
     2461 agcttaacgg cattgctcag aaactgaaat tcaaagcatt acaggagcgg gcaaaagaaa
     2521 agattgccga cattgccgag agccgcgaac tcacggtagc ggagcttgaa gatcggttag
     2581 caccggatct cggtctggat gataacggtt cgttgctgct ggattttggc ccacgccagt
     2641 tcaccgtcag ctttgatgaa accttaaaac cgtttgtgcg tgatgtttcc ggcagccgcc
     2701 tgaaagacct gccaaaacca aacaaaagcg atgatgagtc acaggcgaac gatgctgtta
     2761 accgctacaa attgctgaaa aaagatgcgc gtaccgtcgc cgcccagcag gtagcaaggc
     2821 ttgaatccgc catgtgcctg cgtcgccgct ggtcgccgga aaacttccaa ctcttcctgg
     2881 ttgtgcatcc gctggttcgc cacttaaccc gccgtctgat ttggggcgtt tatagcgccg
     2941 acaaccagct actggcctgt ttccgggtag cggaagataa cagctacagc accgctgacg
     3001 atgatctttt caccctgccg gaaggcgata tctctatcgg cattcctcac gttctggaaa
     3061 tatcaccgac ggatgctgtc gcctttggtc agctttttgc cgactacgaa ctgctaccac
     3121 cgttccgtca gcttgaccgt aacagttacg ccctgacaga agccgagcgc aacgccagcg
     3181 aactgacccg ctgggcaggc agaaaatgcc cgagcggccg ggtcatgggg ctggcaaata
     3241 aaggctggat aaagggcgaa ccacaggatg gaggctggat cggctggatg atcaaacctc
     3301 tcggtcgctg gtcgttaatc atggaaatcg acgaaggttt tgcggtgggt atgtcaccag
     3361 ccgaacttag cgctgagcag ctcttaagca agctgtggct atgggaaggc aaagcggaaa
     3421 gctatggctg ggggagtaat tcaacacagg aagcgcagtt ctccgtactc gatgccatca
     3481 ccgccagcga gctaattaac gatattgaag ccctgtttga ataaggaaag agcatggaca
     3541 aggaattacc gtggctggcg gataacgccc aactggaact gaaatataaa aaaggcaaaa
     3601 caccactcag tcatcgcaat tggccgggcg aaccagtgcc tgttatcact gaaagcatca
     3661 ttcagacact gggtgatgaa ttgctacaaa aagctgagaa gaaaaaaaac attgtctggc
     3721 gttatgaaaa tttttcaccg gagtggcagt ccgccattac gcaggccatc aacttgatcg
     3781 gcgaacacaa accctcagtc ccggcccgca caatggcggc actagccttt atcgcgcaaa
     3841 atgacaacca acagttgctc gacgaaatcg tccaacaaga ggggctggaa tatgcgactg
     3901 aggtggtgat tgcacgccag tttattgcac ggtgttatga gagtgatcct ctggtagtca
     3961 cattgcagta tcagaacgag gactatggct atggttatcg ctcagaaacc tataacgaat
     4021 tcgatctccg actgcgtaaa cacctctctc tggcagagga aagctgctgg cagcgttgcg
     4081 ccgacaaact cattgccgca ctgccaggaa taaccaaagt tcgccgccct tttattgcgc
     4141 tgatcctccc cgaaaaacca gaaatcgcca atgagttggt aagccttgaa tgcccgcgga
     4201 ctcattttca ttctaaggag tggttaaaag ttgttgctac tgaccccaaa gcggtgagaa
     4261 aactcgaacg ctactggagc caggatatat ttagcgatcg agaagccagc tacatgtcgc
     4321 atgaaaacca cttcggctac gcggcctgcg ctgccctttt gcgcgaacaa ggactggcag
     4381 tcgttccgcg cctcgcgata tatgcccata aagaagattg cggcagtctg ctggtacaaa
     4441 ttaaccatcc gcaagtcatc cgcaccttgc tgttagttgc tgataaaaac aaacccagcc
     4501 tgcaacgtgt ggctaaatac agtaaaaact tcccccatgc gacactcgcc gcactggcag
     4561 aactgctggc gttaaaagaa ccaccagccc gcccaagtta tccaatcatc gaagacaaaa
     4621 agctgcctgc acagcaaaaa gctcgagatg aatactggcg tacgctgtta caaacgctga
     4681 tggcatcgca gccacaactg gcagcagaag tgatgccgtg gttaagtact caagcccggg
     4741 cagtgctgaa tagttattta tcggcaccgc ccaaaccagt tattgatagc accgataaca
     4801 gccaaatgcc tgaaatcctc gtttcaccac catggcgtgg gagaaagaaa aagacttcca
     4861 tacgtcactt ttcgctgcct gatcttacgc ttgcaccgca aacgtacagg tcgccgaata
     4921 aacactggac gccggaacag caaactgcaa ttgaccattt tagcgccatg tcttttgacg
     4981 aacgtcttgc tacgagagga gcggatatct tcttgcggga attaggtttt gacgttcata
     5041 gcctggaatt taataagcgt cttcttaatg gacagttgga tgcctttgat gctcagcaac
     5101 tgcaagaata cacatcaata ggactattgc gtacttatca cgcgcatcag gccaaatcta
     5161 aacagtaccg ggaagacgcc accaatgcat tactggcaca agatagcatt gcgttgatgg
     5221 gagggtggcg aacttttcag cacggatttt actatggtaa tgaacagcgt tgggggattt
     5281 ggaatctgta ccttatcgcg cagatgccgc gcgaaatggc ggtatcgtgc tggcaacaaa
     5341 ttgtcgaggc tgattttcat tacaccgggg tggaatattt gttaagtgtg ctgggtaccg
     5401 atgctttgcc tggattaaac accgcctttg cccgacatcc aaaagagata tttccgctgc
     5461 tgattcattt cggcgcaaca gaactggccc tgcccattgc ccgcgtctgg cacctttttg
     5521 cgggccagcg taatctggct cgccagtgga ttttacaatg gccggaacat acggctactg
     5581 cacttatccc tcttgtcttc accaaatcta gcgataaaag cgaagctgca ttacttgccc
     5641 tgcgtttact ttacgaacat gggcatggcg aattgctaca aactgtagcc aatcgttggc
     5701 agcgtacaga tctatggcct gccctggaac aattacttaa acagagtcca atagagattt
     5761 acccggcacg cattccaaaa gcccctgatt tctggcatcc gcaaatgtgg tccaggccgc
     5821 gccttatcac taataatcaa cctgttaccg atgacgctct ggaaattatc ggcgaaatgc
     5881 tgcgctttac ccagggggga cgtttttata gcgggctgga acaactgaaa acgttctgcc
     5941 agccacaaac gctggcagct tttgcctggg atctcttcac tgcgtggcaa caagctggtg
     6001 cccccgcaaa agacaactgg gcatttctgg cgttaagtct ctttggtgac gaaagcacgg
     6061 cacgggatct aacgacacat atcctcgcct ggccacaaga aggcaaatct gcccgtgcgg
     6121 tcagtggcct gaacatcctt accctgatga ataatgatat ggcgctgatg cagctgcatc
     6181 atatatcgca acgggcgaaa tcccgcccct tacgtgataa cgcggcggaa tttcttcagg
     6241 tagtcgcaga aaatcgcggg ctaagccagg aagagttagc ggacagatta gtcccaacac
     6301 tgggccttaa tgatccgcag gcgttgagtt ttgattttgg tccccggcag tttaccgttc
     6361 gcttcgatga aaacctcaac ccggttatct ttgatcagca aaacgttcgc cagaaaagtg
     6421 ttccccggtt gcgcgccgat gacgatcaac tgaaagcgcc cgaggcactg gcccgactaa
     6481 aagggttaaa aaaagatgct actcaggtga gcaaaaacct gctcccgcgt cttgaagctg
     6541 ccctacgtac cacccgacgc tggtcgctgg cagattttca ttctctgttt gttaatcatc
     6601 cctttacccg tctggttacc cagcgattaa tatggggggt tatccggcaa atgaaccgcg
     6661 tcgtttactc aacgcctttc gtgtggccgc agagggggag ttctgcaatg cgcaagatga
     6721 gccaattgac ctgcctgcgg acgctctgat tggcattgcc cacccgttag aaatgacagc
     6781 a
//
LOCUS       DADMEQ010000165         6307 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.167, whole genome shotgun sequence.
ACCESSION   DADMEQ010000165 DADMEQ010000000
VERSION     DADMEQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.167"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            73..378
                     /locus_tag="JNZ05_003668"
     CDS             73..378
                     /locus_tag="JNZ05_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8566723.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /locus_tag="JNZ05_003669"
     CDS             complement(454..918)
                     /locus_tag="JNZ05_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY8566724.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /locus_tag="JNZ05_003670"
     CDS             complement(915..1790)
                     /locus_tag="JNZ05_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HAY8566725.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEATASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="JNZ05_003671"
     CDS             complement(1787..2476)
                     /locus_tag="JNZ05_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAY8566726.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="JNZ05_003672"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="JNZ05_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HAY8566727.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTHKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="JNZ05_003673"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="JNZ05_003673"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAY8566728.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="JNZ05_003674"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="JNZ05_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HAY8566729.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1604 a         1704 c         1562 g         1437 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 gttgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgtgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgctg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttca gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatggtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaattacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaa
//
LOCUS       DADMEQ010000166         6213 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.168, whole genome shotgun sequence.
ACCESSION   DADMEQ010000166 DADMEQ010000000
VERSION     DADMEQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6213
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.168"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            1..173
                     /locus_tag="JNZ05_003675"
                     /pseudo
     CDS             1..173
                     /locus_tag="JNZ05_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(230..649)
                     /locus_tag="JNZ05_003676"
                     /pseudo
     CDS             complement(230..649)
                     /locus_tag="JNZ05_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            699..1553
                     /gene="ltrA"
                     /locus_tag="JNZ05_003677"
                     /pseudo
     CDS             699..1553
                     /gene="ltrA"
                     /locus_tag="JNZ05_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005056916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            1961..2140
                     /locus_tag="JNZ05_003678"
                     /pseudo
     CDS             1961..2140
                     /locus_tag="JNZ05_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2170..2652)
                     /locus_tag="JNZ05_003679"
                     /pseudo
     CDS             complement(2170..2652)
                     /locus_tag="JNZ05_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2908..3888)
                     /locus_tag="JNZ05_003680"
     CDS             complement(2908..3888)
                     /locus_tag="JNZ05_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAY8566730.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3888..5087)
                     /locus_tag="JNZ05_003681"
     CDS             complement(3888..5087)
                     /locus_tag="JNZ05_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAY8566731.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5409..5747)
                     /locus_tag="JNZ05_003682"
                     /pseudo
     CDS             complement(5409..5747)
                     /locus_tag="JNZ05_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5814..6213)
                     /locus_tag="JNZ05_003683"
                     /pseudo
     CDS             complement(5814..6213)
                     /locus_tag="JNZ05_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         1677 a         1430 c         1484 g         1622 t
ORIGIN      
        1 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
       61 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
      121 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat
      181 aagttatcca tgctctgagg tgggtggagg tgggccctcc ggtcagggtt tacaggagca
      241 ctgaccaaac gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa
      301 aaggcagaga tctactgtca cgtggggttc gggaagctga acgaaagtaa actgtccgat
      361 gaagcatcga aatcttagat gatgccaaaa tggggccgta cagccgtccc ctgataagcg
      421 cagaagacac ctgcttactg actgcgcggc atccggtgtg aaggcagcag gatcccgggc
      481 cagaccatct cagggaacgt gagaacctgt ttccggcacg tatcgggaaa caggggcgga
      541 cccattcgta gtagtgatga aagatctgtc atgggtctgg agcgaaggga tggggatcac
      601 attcattgca gaagacacaa cccaaaaggg gatgacgtaa ctgatgcaac gaaaatcatt
      661 tgaaatacca aaagcactgg tctgggcatc atatctggat gtgcgtcgca acaagggagc
      721 cccccggatg cgacgggcag actttgaaaa tgttcgacca gcaacgtgat ggcaatctat
      781 acaagatctg gaaccggcta tgttcgggaa catggtttcc gccaccagtg ctggaaaaac
      841 ggatcccgaa gtcgaatggc aaggaacgca ttctggggat cccaacagta tccgatcgaa
      901 ttgctcaggg agcgataaaa cttttcatgg aagaaaagct tgacccgatt ttccatgctg
      961 attcatatgg ttaccgacca ggcaaatcag ctcatgacgc tctgaaacaa tgtgccatcc
     1021 ggtgctggcg ttatagctgg atactggaag tggacatcag tgcgtttttc gaccatgtga
     1081 gacatgatct ggtgctcaaa gctctggaac accacgggat gcctaaatgg gtcatcctgt
     1141 actgtcgacg ctggatggaa gcgccaatgc agagttgtga aaatggagaa ttaataaccc
     1201 gaacacgagg cactccgcag ggcggggtca ttagtccgct acttgctaac ttgttccttc
     1261 actatgcatt tgatttgtgg atggaaagag aatatcgggg ggtaccgttt gagaggtacg
     1321 ctgacgatat tgtagtacat tgttcacgaa tgagtgatgt aacaaggcta aagaacaggt
     1381 tatcggagcg cttctcggaa gtcgggctgg tcctgaatgc agggaaaacg aatatcgcct
     1441 acattgacac gtttaaaagg agaaacgtcg caacgagttt cacctttctc ggatatgact
     1501 tcaaagtgcg tacgctgaag aatttcaaag gcgaacggta ccgaaaatgc atgccgggtg
     1561 cgtcaaatgc agcaatgctg caaaataaca gaaacaatca agaagtggcg tatacatcgc
     1621 tcaacagctg agagtttgct ggattttgcg aggcgctaca atgcgatagt gagaggctgg
     1681 atcgagtact acggaaagtt ctggtccaga aatttcaact atcgactgtg gagtgcaatg
     1741 cagtcacgtc tgctcaagtg gatgcagtct aaatacagac tttcgaaccg gaaggctcag
     1801 cgaaagctga cgctggtaag gaaggagtat ccgaagctat ttgttcactg gtatttacta
     1861 cgtgcatcaa atgagtgatc aagagccgta tgacgcgaga gtgtcacgta cggttccgtg
     1921 agcagccggg gagtgaaatt ctctccggct gactcgactc gcgggttccg acagcgatga
     1981 cgaacatgcg gcggtgtttt actcgctgat cggcacatgc cgtctgaaca atgtggaacc
     2041 agaaaaatgg ctgcgttacg tcattgagca tatccaggac tggccggcaa accgggtacg
     2101 tgatctgttg ccctggaaag ttgatctgag ctctcagtaa atatcaatac ggttctgacg
     2161 agccgcttac ttttggcgta attctgctgc gcctttactg aacacaatgc cgtattcagc
     2221 aagaagtcca cgaatgtgat ttatcagttg tgtcctttcc attgaaacca accgacgcat
     2281 attcaaaaaa gtctggacgt cctgttgctc cagagttttt attggtacgg gtcgtatagt
     2341 tccatgttgg caggcctcag ctattgcctg tgcgtcgtta taatcatttt tctggcaccg
     2401 ctgataagct ttaacatgtt gtgccggaag caatattaca tcatggtcga gtttcctgat
     2461 ttcccgggcc cagtaatgag atgccccgcc agcctccatc gcaacagtca ttttaggctg
     2521 ttgcacctgc cagttaagca gttgattgcg agaaagtttg gtctttcctg cgggtttgcc
     2581 atgagcattg atactgaaaa gataaaagtt cgttttagcc agatcaatgc cggtgatttt
     2641 ctgttcagac atggtatccc gcttactttt gtggttaaag tttatccatc atggcgcaat
     2701 tgaaggctgc cgtgaaagag ggggaagact atctcatcgg ttactgttaa acggctaaga
     2761 gaaactatct ttttcaagca aagaagatac ggtgaaacag gcgatttcac cgtgaaatac
     2821 tgttcaaaaa aaacgctaat aaaagcaaaa tttcttttaa aacagccaat taaaaactgg
     2881 tgagttgctg gcgaaaaact tgaattttca cagcgaattc agattatctt ttagtacaag
     2941 agcaatcata cgatctaact tatcctgaac ttccaacggt aatctgccga attcatatga
     3001 gaacgtacgt ccctttactc gttttcgagc aaatttatct ttactatcaa atttccataa
     3061 caaagttaca accgctttat cctttactcc tgaatcaagc aatgaagaca tttccttggt
     3121 tataagccgc ataatactgt ttttaacctc gtcttcagaa agattgtcat cagcagtaat
     3181 aagctcaata ctcggcgaga tattcttcac aacttcatca atagatatat cacccagcct
     3241 cagtcgttca cttaattcag ccagttgcct gtaatcggcg tatgtcagct cagactgaac
     3301 aggaaaaaga gacacaaaat ctttaggtac ggacgctgct tggagggcac gagtcacctt
     3361 agctgccgac aatccctctc tttccgcaat ttgcttctgg ttcataccgg cctctttgag
     3421 acgtactaaa cgcaacccaa tttcacgaat attatgttca agtgacgtct gcaaatcttt
     3481 cgccagatgc tgagcttcag aaacagtaag ctcatcatcc gttacaagta cacgtaatcc
     3541 aaccttgcat agcaaagctg ctgcccgacg acgggaacca tccaaaatct caataagatc
     3601 gccagtccga atcccaatac atggatagaa ttgttgtaaa cgtatagttc tggtgatgtc
     3661 ttttagtgag tccggcgtca gggctgtctg ttcgcggcca ttcacctctt caaccacaaa
     3721 agtttgagtg tccactttgt catgatcaac cttgatctca gtaaattttg cctttcggcc
     3781 agtattgagg gtaaatacat gaactggagc cgatatttct tcagtattat tcaggatatt
     3841 tgtattcagc gtacggccaa tagtcggtcg gtgctttctg ttttccatca gtagttggcc
     3901 cttataaatt caatacgatc aaaaactgct ttagcgaaat cttctgctgc catacgtgca
     3961 tttttcaaag cttcaccact gccaacataa gtaacaggat tcgcagatat cacggtatca
     4021 aacgactcgc cgcttctctc aaaaccatca agacgtggca ttgaaacatc aagcatgtca
     4081 ccaccgaaaa tctcttttgt caggctatgg cagtatttat gatctgactt attcgccagt
     4141 ttcgacatga agccaatatt tgcctgcaaa cggcatgaac atccatcctg ttcaataatc
     4201 tgtacaagtt caggtaaacg agccaaatat ttaagcgtgg aatggaaatc aacttgtgct
     4261 ggcggcaccg gtgtaaacat gatatctgcg gcagcaatgg catttttcaa aaatgcgtcc
     4321 agatgcgggc ctgtatcaat aaggatgaag tcgaaatcat gcttcagctt atcaataata
     4381 ttctcacgca atatcgcgtg tttgttttgt cctaatagat gctcctcgca cagagtatcc
     4441 cagtttgatg caataaaggc gtcgtcaata gaagccggca taacataaac gccagggata
     4501 actgacggaa cgataaaatc ttccagtaat tcctcacgac tcacgttctg caacatagcc
     4561 tgcggtgcgg ttgtatcaac cagccccacc gcatgcaagt agttcagaaa catagtggct
     4621 gatgactggg gatctaaatc aaggagcaga atacgtaaat cttctgacag caggtgagga
     4681 tgagcacgca gggcgtgagc tacggaaaca ctactcacgg ttttagacac accgcctttt
     4741 agactaccga tgaatatgga atatgcctca ctataacgat cgcgatattt aggaatgcca
     4801 cgatgtgcat agatatcgat aatgttctgg atggttaatg catattttgt cgcggttcct
     4861 gctgggcgtt tttcaaactg atagccctga gcctccatct caccgactgc accatccacg
     4921 cttgcgcgcg ttaattttgg aagcttagct acagcagact ttgaatagac ttgataaaaa
     4981 acatcagcct gaaactcctg cttttgctcc tgaatctgtt ttgttagggc gagcaacatt
     5041 ttatctgcac gctgcgcaac cttacttaac tgctcaaaac tagtcattcc ccctccgtgc
     5101 tatgtaggat tttaacttat tacaaaaaaa actgcattag cagggtaatg catgcaagta
     5161 catattgtac atgcttatat taaaaatgcg cgcattgctg atttattaat catcggaatg
     5221 aatattactg taagatagta tgcctttccg ggagaaagac gctgatttcc cccgaataat
     5281 ccagttcacc accacactcc ggacaacagc tttcttcagg ctccagatgg tgtatttcgc
     5341 gggaaacatg ttccggtaac ggacggcgat gacgtgactg tcgcaacagg cgggggtagt
     5401 gcttccaatc agtagaacat gtgcttttca atgaaggagc cgatgacttt ttcgtggatt
     5461 tccacggaac gcgagaagca gattgtttta cgtgccagcc gcttgatgcg ggtgcggagg
     5521 gtccggttgt tacgttcaat acgttgcgtg aagattttgc cggtcaggtg tttctccttc
     5581 ggcacttctc tggcgtagct gccccagtca tcgctggtca ccatgccgat acagaacgga
     5641 gtcaacaggg ctaacagttc ccgacaggtc tcatcattcc gtggcccgaa agtgtaagcc
     5701 aacacaccgc cagttttagt gttgtacgca taccagagcc agtgttgtgt caacgacgga
     5761 tgaaaagtgc tccacttata tctccaccaa cggcccaata ttgatccacc gttttactca
     5821 ggattagctt ctgctataac cccggccttt cgtttctgtc tgagtcgata gctttctcct
     5881 ttgatttgaa cgacatgtga gtggtgtaag atacggccca gcatcgctga ggtcagtgct
     5941 gcatcaccgg cgaacgtttg atcccactgc ccgaacggta gattggatgt caggatcatt
     6001 gcgctctttt cgtaacgttt agcgatgacc tggaagaaca gttttgcttc ttcctgactg
     6061 aacggcagat agcctatttc atcaatgatg agcaggcggg ggccattact ccacgctgaa
     6121 gcgtcgtttt ataacggccc tgacgttgtg ccgtagataa ctgaagtaac agatctgctg
     6181 ctgttgtgaa gcgaactttg atacctgcac gga
//
LOCUS       DADMEQ010000167         6062 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.169, whole genome shotgun sequence.
ACCESSION   DADMEQ010000167 DADMEQ010000000
VERSION     DADMEQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.169"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..1431)
                     /locus_tag="JNZ05_003684"
     CDS             complement(<1..1431)
                     /locus_tag="JNZ05_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HAY8566732.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNY"
     gene            complement(1469..3154)
                     /locus_tag="JNZ05_003685"
                     /pseudo
     CDS             complement(1469..3154)
                     /locus_tag="JNZ05_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            3357..3479
                     /locus_tag="JNZ05_003686"
     CDS             3357..3479
                     /locus_tag="JNZ05_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566733.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            complement(3445..4600)
                     /locus_tag="JNZ05_003687"
                     /pseudo
     CDS             complement(3445..4600)
                     /locus_tag="JNZ05_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
     gene            complement(4652..5990)
                     /locus_tag="JNZ05_003688"
                     /pseudo
     CDS             complement(4652..5990)
                     /locus_tag="JNZ05_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
BASE COUNT         1695 a         1277 c         1324 g         1766 t
ORIGIN      
        1 atagttgtca tagtcataac gcacaccggg cattaacgac acattccgcc agctaatgtg
       61 atccgccata tacagcgtgt agttgtcaat tcccaggctg cctttacctt tatggtaaat
      121 ggtatggtta gttttctttc ctgcagcatt aatcacatag gattcagact ggttatggcg
      181 ttcagtccat gcatcggaat agatgtattc cgcgccgaag tagggttgat gcgaaacatc
      241 acccacggcg aatttttgcc agtccaggcg tgttttgaag gtgtaattat ctacagcctg
      301 ggaaatgtgt cctaatccgc cacgggtaca acgccctgta atatcaccat atgtacatga
      361 aagttcggtg taccagatgt catgatcgtg acgggtataa tcactaatat gatcccaacc
      421 aacggtggta cgtagtttgg cccatgcgag ttgcgtatcc atatcccatg ccagaccata
      481 ggatttatta cccatttcgc gatcagattg cgggaaggtg ctggtattat aatcacggct
      541 ggagccggta tattttaagg ttaaatcgtg ggtaaagcgg tcgctggcaa accaggtaaa
      601 tttgctcaat gcagtatcga taacgttttt atactgtgcc cgaccggcga caatgccgtc
      661 attcgaaaca taatccgcgc gggtgatatc agactggcgg cgcgataaac cggcggtaac
      721 gccaaagtta tcagccagct cctgattaaa cgacaaggta taaaagttct ttttaaaatc
      781 tggagagaaa taggtacttc ctgaagaacc ttgattaaat gcgctcttgt tattctcatc
      841 gatatgcgat gttaaccagt ccaaacgcgt agtgcgataa cccaatttca ccttgctatc
      901 atcagcgttg aagcgtttga tctttgcatc aattaccccg ccattgaagc gaccaaattc
      961 aaccggcaca aaactgtcat aaagcgtcac attgtccagt aagctgacat caagataata
     1021 cccctgtgac atcccgctaa tattggttgc actactggca tcggactcat tcgctgggtt
     1081 caggttatta gttgcactaa taccgtcaat caaataggca ttctggtagg gcgacgcacc
     1141 gtgaatagag attttctcag ggcgaatatc tccctggttc aacgaggtac tttgcgttga
     1201 atccatgcgt accgcagggt tggttctcag cagatcgctg atattgccat tcccggtcgg
     1261 taaacgctcg atgctttcac tggtgtaatg cgtgttgccg tttacgggta cagggacagg
     1321 agcatagacc gtcatctctt caaaaaaatg catgtacatg tttttgtcat cgacggcctg
     1381 cgccacatca gcgccacata aaatgacgcc aggaataaga actcgcttca tttatttata
     1441 tctccgtaca gcagggcttt tattgttgtt ataaaaccgt gctaatgtca caaatatcat
     1501 cagccaggtt ccagacaccg ggttgatgtg taaccataat gacgccgctt gtgggtagtt
     1561 tttcacgcac taaacgcagt aagcgtatag cctcttgttc ctcaagatga gaggtagttt
     1621 cgtcaagaaa tatccatttc ggacgtcgta aaattaatcg tgccagggcg atacgttgtt
     1681 tttcgccgct ggaaagaata tctccccagc gatcgtggtc atgaatacgc gcagccaatt
     1741 tccccagacc aacctgatgc agtacttcgc tcaacgattt atcgtctacg ggcaggggaa
     1801 gtgctttaca aataatctct ttcagtaagc cgcttttgat taacggtgtt tgtgacacat
     1861 accaactgtc agcaggagaa gaaatatcac ctttaaatca cggccagcag tgggataatg
     1921 ttttaagcag tgtggttttt cccgcaccag agtagccttt cagtaatagc catttgcctg
     1981 gcgaaacatg aaagttcaag ttctctaata tgatcttatt atcaggcgta cgaatactcg
     2041 cattagccac ttgtaccgca tgttggcaat ttttaggctt attcgtaggg cgctattcag
     2101 tgagttgatg gaactcatac aagcgatcga taaccgcagc cagttcagca agttcgtcat
     2161 atttataaat aaaccagctt aaattgttcg atactagcat aaatgcctgg cgcgatttca
     2221 tcagtccgcc cagattgatc tgcccgctaa taaactgcgg caataacaga aagtagggaa
     2281 gaacgctaag cgagcgcgaa taaatatttt gccagtaatc aagccaccgc tgacgattca
     2341 ttaaacgatg ccagttctct ttaatggtat gaaaattgtc gcgcaactcc tggcgttgta
     2401 gagactcagc attgcttagc gcaatcagtt ctgcctgctt attatgctgc acaagattag
     2461 ttcgaaatgt cgcttcgcta cgttgttttt ccacattaag cggacgaata cgtttaccca
     2521 ccttatgggt aaataaagtt ccaccgatca caatgagcac gacggtatag accatatacc
     2581 cctggatatt ccattctgtt ccaccaacag tgaatgagag cgtacccgcg ctttgccaaa
     2641 gaataacggt aaaggtgatc agcatgctaa gtgactggat gaagccaaat gaaagactga
     2701 gcgttttgtt gatcagtaag agaatgtctt cagcgatacg ttggtcaggg ttatctgtat
     2761 ttttatgctc gccgtagatc tgcgtgaagt agtagttttt atctgcaaac cagcgattaa
     2821 ggtagtaatc tgttaaccat tcgcgccagc gaatagtcag tagtttaatt aaccatgttt
     2881 tgtttacgga tattaataca aagatcccca gcagggcagg gaaccaaaga acaagctgcc
     2941 aaagcttatc ggtttctttc tggcttagcg cattgaaaaa atcattattc cagtcgttta
     3001 accagacctg aattttaacc acgccaagga tcattgcgag gataataatg attaacaaca
     3061 ctgaagtttt attattctta cgcagccaaa agggcttcag cagacagaga tactttgcta
     3121 tcaacataca aaacgtaatg ggaatggtta tcattagcga aaattgattg tgccatccaa
     3181 tgattatcta tggcaatgat ttactgatga aatttattta agtatcattt aacaatatga
     3241 aatatataga aatttttaat tttcagtggt tatggttatg ctattgataa tcaagggtat
     3301 tgctaatgcc ctgacgcata ccacgtaagc caggattttc gcaagggaag atgatgatga
     3361 cgaaacaccc gacaggaatt tatgtcgggt gccttgttaa ggtcataaga aggaggctaa
     3421 gaatggagtt aaaagagagc gttattaatt attctccatt tgttttgcaa catccataat
     3481 gtggtaaagc ggtactctgt tcttagctaa ctcaaccatg gcgttcatat aaggtaccat
     3541 ggttgaaaaa aggattttat aaccttcgca aaaataagaa acagtctcat tgtttacttt
     3601 agtaatacga tgcttaggac aaccgccatt gcagataggt ttatatgcac attgctgaca
     3661 tttcgctgaa atccgttttt tgcgctgtca gttgtacact gttcatcgtt ttgagttcag
     3721 atttattaat gtttccaatt ttgtactgtg gatagacaaa atggtcgcat tcgtaaatgt
     3781 ctccattact ttcaacaacc agattatcct tgcaggactc ctggaaaata caacttgtat
     3841 gcccattccc caaaaaacgg ctgacaaagc tttcaaactg acgaatgaaa atttcaccca
     3901 catcgttttt aacccattgc ataaaaatgg ttgacataaa cttgccataa gccgtgggag
     3961 gcacagaaaa atcaatgata cggaatgtgt tctcactatg accactgaaa tcaatattcg
     4021 gcgtcccggt ttctagcaat tcgataaatt gcatatgttt actgccgata gattttaaaa
     4081 aatgataaac ctcaagaggg taatggacat taacgttatt aatgacggtt aacgtattaa
     4141 actctacttg atatgatttc agacgctcga tggctgctat cacttttgca aaagtaccgt
     4201 tacctgaatt actgcgtctg taacggtcat gtaactcctg ggggccatcg atcgagatac
     4261 caaccagaaa atcatgttct ttgagaaagg cacaccattc attattcaat aaaatgccat
     4321 tcgtttgtaa tgcattaaaa atacgttttt ggcctgcata gcgttgttga tagtgaataa
     4381 ctttacggaa aaaatccagg ccagccagag tgggttcacc gccttgccag gtaaaataga
     4441 cctgattgcc agatgcttcg atatattgtt tgatgaactc tttcagagtg ctgtcatcca
     4501 tccatttttc atgagtaaac tgcgactctt tttcaaggta aaaacagtaa tcacatttga
     4561 gattacattg aaaactggag ggcttggctg taacgtgcat tgctatctcg ctcaataagg
     4621 cggcggaaaa atccgccgca tgaaggttta gttatttcgc ttcgcttagt gctttcttga
     4681 tattgttaaa cttctcctga tttacctcgc taagcggtgg ctggctgctg tcgataaact
     4741 ctcttaccac gccttgcatc tctttaacga cctgcggatt ggcggcggca aggttatctt
     4801 tttgctgtag atccgtcagt ttgtagagac ctaactgatt gttttctact gtatagacaa
     4861 gcgaataatc gttatttctc accgtataag agaattggct taagtcctca gtgttggggt
     4921 tatgcgggta atcgtctgac tgatggcgaa caaatttgtg gtaattatcc cagaatggaa
     4981 tattttcctc atcaaaccag tgagaataag aggttatcca ggtcagattt ttatgtggct
     5041 cgccttgttt cttatcttgc aaccagggca gcaaggaaac accatccagc ttaaggtctt
     5101 ttggaatgct gatatcggct gcatcaagag ctgttgggta gaaatccatt gcggaaatca
     5161 gcttgtcata attaccgggt tgaagttttc ctttccacca cataaacatt ggggtggtga
     5221 gtaccgccag gataggtctg actcttatag cctttttgcg ccccgttcag cggcagagga
     5281 ccatcgataa ccgcaccatt atcggaggta aagagaataa ttgtattgtc atactgtccg
     5341 tttttcttca gttgttcgag aatgcgtttt acaccctgat caacagaata aacggaagcg
     5401 tagtagttat ctgctgtttg actaccggta ttaaattgct tctgatattg atccggtgca
     5461 ggattatcat ttggtaggtg cggagcatta taagccaggt aaagcataaa aggctggtca
     5521 agtgttttgg cacgatcaac aacgccaatt gcctcatcgg ttaactgatc gctgatataa
     5581 ccttttgcgg ggacacgttc acgatttttg aacagtgaag gggagttgta atatgccgtt
     5641 cctgcagcgt ggaatcccat aaagtaatca aagccacggt tttgaggttg ccattcttcc
     5701 gcagaaaatg tggtgaagtt gtcatgatag tcacgcgttt gtttatcttc cggtaccggc
     5761 acattactga tttttgacaa gtgccattta cctactgctg cagtgtaata accatgattc
     5821 tggaataatt caggcaagaa agtttctgtt agcggaatac catcctgagc atcggtattg
     5881 gaatagacac caacgcgggc gggagctcga ccggtcatta ttgcggcacg ggaggggccg
     5941 gaaacaccgt gtgccacata gccgttagta aaacgtacgc cttcatccat taatgaaagg
     6001 agcgtcggcg ttgatttttg tgcagcttca atggctttat ctatccctat tttgtaggta
     6061 tc
//
LOCUS       DADMEQ010000168         6029 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.170, whole genome shotgun sequence.
ACCESSION   DADMEQ010000168 DADMEQ010000000
VERSION     DADMEQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.170"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(250..1020)
                     /locus_tag="JNZ05_003689"
     CDS             complement(250..1020)
                     /locus_tag="JNZ05_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566734.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPLLPADGTQVSF
                     PYACEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVRDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1065..1499)
                     /locus_tag="JNZ05_003690"
                     /pseudo
     CDS             complement(1065..1499)
                     /locus_tag="JNZ05_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(1511..1732)
                     /locus_tag="JNZ05_003691"
     CDS             complement(1511..1732)
                     /locus_tag="JNZ05_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566735.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(1733..2416)
                     /locus_tag="JNZ05_003692"
     CDS             complement(1733..2416)
                     /locus_tag="JNZ05_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAY8566736.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(2480..2791)
                     /locus_tag="JNZ05_003693"
     CDS             complement(2480..2791)
                     /locus_tag="JNZ05_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566737.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(2788..3759)
                     /locus_tag="JNZ05_003694"
     CDS             complement(2788..3759)
                     /locus_tag="JNZ05_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HAY8566738.1"
                     /translation="MRHGLMEAACERRIPMPNWCSNRMYFPGEPAQIAEIKRLASGAV
                     TPLYRRATNEGIQLFLAGSAGLLQITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTH
                     LQNGVLLDEQNCLMLHELWLQSGTGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNE
                     DVSVWWNRLCDNVVPEKTMPFDLLTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYG
                     VKWPCGYDLNISSQGENFIQVDFDTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFC
                     GWQLYERGELVDVLWGELEWSSPTDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4130..5089
                     /locus_tag="JNZ05_003695"
     CDS             4130..5089
                     /locus_tag="JNZ05_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY8566739.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5089..5484
                     /locus_tag="JNZ05_003696"
     CDS             5089..5484
                     /locus_tag="JNZ05_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY8566740.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            5667..>6029
                     /locus_tag="JNZ05_003697"
     CDS             5667..>6029
                     /locus_tag="JNZ05_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAY8566741.1"
                     /translation="MTMATINARIDDDINNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAE"
BASE COUNT         1636 a         1578 c         1470 g         1345 t
ORIGIN      
        1 ttaccgtctg gttttgcgaa gattgattat tgatgacatg aacggcaaat cgacctgatt
       61 aaaaacctaa caggggaaaa tggctcactg aagccgaggg gatttttagg attatcaggt
      121 gcggaactca tcgaggcgcg ggcatccctt gcccacagag acgccggata gatttaagca
      181 agcccaccat taatcaaaaa tcagtgttgc aaaaacgggg gtgaccatag attttccccg
      241 ccatcgtcat catttccgga tttttgccag ggcatccgcc atgctgcgga tttcggcccg
      301 ggcttcccgg acaacttccc gcagaacacc acggtcaaac caccggcggg gcggttcatc
      361 cggtatacgc agcacatcgc aggccagccc actggacagg atatgctcaa tacaatctct
      421 gaccagatac atttccaccg ccgaaaaacg tgcgccccgg ttcaggatgg catcgagcac
      481 cacgggcagg ttttcatcgt cttcatcgag ccagcagggg tgatcgcttt ccttcacgac
      541 caggcgaaag cggcgcgtct ggcgggtcag caacgtctga ccgcttgtgg tcagcgccgc
      601 ccggatacgc cgcgcatctt ctgccccccg gcaactgaca ctgcatactg catcgcgcac
      661 cgcatcaaga actgccctga tttcgtcttc ggtcagccat tcacaggcat acgggaaaga
      721 cacctgcgtt ccgtccgccg gaagtagcgg cagcgactgc cactgactgc cggacagaac
      781 aagttccgct ccggttccgg tcagtttctg aatggcggag gccaggtgcg gcggcagcac
      841 gccatcgtgg cggttaacga gtaacgtcgc cagccggggc gcatccagcc gcacgtaaat
      901 gctgatccag cccggatagt tcgggttaca ttggtcaaca tccgtctctt ccggccagcc
      961 ctgcgccagc agcaggttac gggcgtgacg acaaatcaga gcattcagcg tttcattcat
     1021 aattcatgaa ctccctgtat cgccgcccgc aggcggcgat aacatcagtc gttttcgtgt
     1081 gtgttgtttt tcagcaggct gcgggcctga tcaaccatcg cctgtcggcg ggtaacgtat
     1141 tccgggaggg ggaaaccgtg atcccgggca gcccattcac gtttccacag tgcctgttca
     1201 gcgatctgag tcagggtctc cagcagttca gcagggacgg tcacctggcg gttttcgcgc
     1261 acgttgctga taatctccat cgccgcggcg gaagaaatac cggactcaat ccgctagtct
     1321 tccgggatgt cctccaggtg cgccaggacg gtcctgattt catgatcgga cagggaaata
     1381 tccattccgt cagcgagggc ctgaatctct tccggggacc agataaccag catcagcggc
     1441 gtgtctcccg aatactttgc tgccagcgcc cggcatagcg tttcgcatgt tccgtacata
     1501 ctctctcccc tcaatctttg aagcaggaat ccagttccag aatttcggta tatgcctcca
     1561 tcaagaggcg gtttgcgcga aacaacgtct gacgcaccag acgttccggc agggtgtcag
     1621 cattaaagct gtaccgccgc tccatctcat tcatggttgt acgcaggtcg cacatggcat
     1681 tggccacctc cgccacttcg gcgcgaagtt tttcgtgtcc ggcatagttc atttacgcag
     1741 cctccggcat aaaccagtca tcattcgcgg ccccctgctg catggcccgc tgtacggcag
     1801 ccagtcgttg ctgtccggca cggtgatact gctcaagcag ctcgataccg atataccggc
     1861 gtccggactg gagggcggcg acgcaggttg aaccgctgcc tgcaaacggg tccagcacta
     1921 tcgcattcgg gtgtgtgaag ctctcaatca gcggttgcag gctggtaacg ggcttttccg
     1981 tcgggtgatg gcggttgccc gaatatttcc agcccaacac gtccggcagc gggttttgtg
     2041 gcagacgtgg acggcctttt gccaggatgt aggcgcattc gtggcgatag cccacatatg
     2101 cggccttcga tgtgtagttt ttggtgaaga ccaggtgacc aacaacgctg aatcccgcat
     2161 ttttccaggc agacataaag cgatcgacgc ggttccagcc atagaagctc accatcagcg
     2221 cgtctttttt gagtacgcgg tacatttcat tacaggccgg ttgcagccat tcatcagttt
     2281 tatcaccagc aatggtacgc ccctgacggt cacgaaaacc gacgagatat ggcggatcgg
     2341 tgaggatgaa atcaatggcg ttatcaggaa tacgggccat aacatcgatg cagttaccga
     2401 ggacaaaacg ggacataaca gatccttctc ttacagaggg cacccctgtg gtgcccgttc
     2461 cccgctgcct gtgggctgtt catcgcgtcc ggtaacgggt caggccgacc gtggaatacc
     2521 gcagccgtcg tctttttgac agcaaggtta tgccgcgaaa gcggcttgaa gggcgctcat
     2581 cagaaaaggg cgacgggctg gcgtacactg tcaccgacgc cagccgggag cggtaaaagc
     2641 gcccgtcccg tgcccgccgg acgcgggaca ctgaccacag gcagcgttta tgccgcgccg
     2701 cgtcagccgg cgcagtaaaa agaggagcac ggcgacatcc tttgtcggaa aagaaacggg
     2761 cggttgcccg cccgtctccg tcatacttca tccgccataa tgcgccacat tgtcgactat
     2821 ccacgcaggt ccggtgactt ccggctgctc atcgtcatct gtcggggaag accattcaag
     2881 ttccccccac agcacatcga cgagctctcc acgctcatac aactgccagc cacagaaatc
     2941 acagccctgt tcggcatacc agtgctccag cgtgcagctg aaacggcggc ttaattctgc
     3001 aataacgtcg ctttccggct gacaccacgg cgtgtcgaaa tccacctgaa tgaagttttc
     3061 tccctgactg ctgatattca ggtcatatcc acaaggccac ttcacgccat accgttccgt
     3121 ataccagtga tatgcagaag gaacaccgtt cagaacacca ccattaaagc cgttcacttc
     3181 aacatccagg cgggtcggca gaaccgtcag caggtcaaac ggcatggttt tttccggcac
     3241 tacgttgtca cacagacggt tccaccacac agatacatcc tcgttacccc atatgtcaca
     3301 ccagtcacct ctttttgcgg tgaaatgcac agtgatggtt tccctggcat catccggtaa
     3361 tccttcccag cgacgctggc cagtaccgct ctgtaaccag agttcatgca gcatcaggca
     3421 gttttgttca tccagcaaca caccgttctg caggtgtgtc agccagcggg tgaacgcgat
     3481 attttccgtc gatacagcac cacgtccggc agccgtcact ccggggcact gttcagagcg
     3541 gatattttca gtgatctgca gaagtccggc acttccggcc agaaacagct gaataccttc
     3601 atttgtggcg cggcgataaa gcggtgtgac tgcaccgctg gccagtcgtt taatctcagc
     3661 gatctgtgcc ggttcaccag gaaaatacat acgattcgag caccagtttg gcatgggaat
     3721 tctcctctca catgcggcct ccatcaggcc gtgccgcacc tgtaaccggg cgaccgcata
     3781 cgaatgcagg tgcggccccg aagccgcagc gcgtggagga ggaatgacga aacgtgaccg
     3841 ggtgaggagg ccggcgacag cgacacactt gcatcggatg cagcccggtt aacgtgccgg
     3901 cacggcctgg ttaagtagta tccgtctcag ttcgtggcgc aaaatgagaa accaataaga
     3961 actcaaaatg aaccacaaca agaacataaa gagactctaa aagagcaaca ccgcttgaca
     4021 tagactcaaa atgagtcaca ataagaaaca ataaaaaact caaaaagatg caccgatata
     4081 aaggatgctc aatttgagat aaacacaaac tcaaaacgag gcaaaaagaa tgctaaaagt
     4141 atcatgcgat gatggttcaa ctaacgtaaa actggcctgg cttgaagatg gcgaggtaag
     4201 aaccagtctg tcaggaaaca gctttaaaga aggctggaac ccgggactgt ttaatgccgg
     4261 caaagtttac aattacgtcg tggatgaaaa aaaatacacc tatgatttgg gaagcactgc
     4321 tgtcataggt acaacacatg tcagttatca atacagcacc accaatctgc ttgccataca
     4381 tcatgcactg ctgacatccg gccttcagcc acaggatgta gaactgacag taacattacc
     4441 tgtgaccgaa ttctttgata atgacaatca accaaatgaa gaaagaatag aaagaaaaaa
     4501 ggcaaatgtt ctccgtgaga tatctctgaa taaaggggaa acatttaaaa ttaaaaaagt
     4561 taatgtaatg cctgaatctt tgccagctgc atttgaatca ctaaaaaaag ataaagtaaa
     4621 caaacttgaa cgcagcctga ttatcgatct tggaggaacc actctggatt gtggtttaat
     4681 tctcggtgca tttgaaggca tttctgaaat ccgtggctat tcagaaatcg ggacatcccg
     4741 catcactcat accgtcatga atgcgctgac gaaagcctcc acaccatgta attattttat
     4801 tgctgatgag ttaataaaaa acagacacga taatgaatat ctccagacat taataaatga
     4861 tgtagctgaa ataaaaaaca tttctcatgt aatagacaga gaagtgaaat ctctggcaga
     4921 aagcatcagg caggaaattt caacattctc aggaatgaac cgaatctatc tcactggcgg
     4981 aggagccgag cttatttacc ctcacataaa gcaatatttc ccgaatctga aagtgaacaa
     5041 ggtggatgaa cctcagtttg cattggtgaa agcaatggtg catgcgtaat ggaaagcagt
     5101 gatccaaaaa aaagaaagaa agttgtggca tacctgcacc cggcacttta tccgcaggac
     5161 aacctgacac aacaaacaat agactctctc ccagtacaaa tgcggggaga tttctacaga
     5221 caatccctta tttgcggagc agctctttat tctgttgacc cccgactgtt aacgctaatc
     5281 agcggttttt tcagtgagaa aatcactgca gaaaatctgg tgaaacttat agaacaaaca
     5341 acaggataca catccacatc aattgatata agtgtgttaa aaaacatcat agaggcatct
     5401 tcagaaaaca aatcagagag catcacttca aaagatgatt tcgaagaaca aaccagacgc
     5461 aatctttcta tgttaaaaaa ataataaaag aagagcaggt tcagtcaatt acctgctctt
     5521 cttttataca tactaaaaaa tcagattttt acgaatattc agattatcat aaaaaatgca
     5581 acagacagga atgcaataat tttgcattat ctcgttgctt gtaatgcaaa tatgcattat
     5641 tgtcagtgtg tgacaataac agagagatga cgatggcgac cattaacgca cgtattgatg
     5701 atgatataaa taaccaagca gatgaagtgc tgaagctgat gaatatcagt caaactcagg
     5761 ctattgcagc tttttatcag tatattactg aacaaaagaa actgccattt gtgatcacct
     5821 caattgtaaa aacgccgcat gatttattac gtgaaagtac agacatgtta gctgaagctc
     5881 ttgcagtgat ttccaatcta caggtgtgga ctgaacagca ggatgggatt ggaaaagcaa
     5941 agctaatgga gtattaccgc cgcctcgacg cactgtactg ttgtgcaaaa gagaaaatag
     6001 gtcttctttc agataaccgg gatgctgaa
//
LOCUS       DADMEQ010000169         6019 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.171, whole genome shotgun sequence.
ACCESSION   DADMEQ010000169 DADMEQ010000000
VERSION     DADMEQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.171"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            241..513
                     /locus_tag="JNZ05_003698"
     CDS             241..513
                     /locus_tag="JNZ05_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HAY8566742.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(479..886)
                     /gene="nudG"
                     /locus_tag="JNZ05_003699"
     CDS             complement(479..886)
                     /gene="nudG"
                     /locus_tag="JNZ05_003699"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HAY8566743.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            973..1629
                     /locus_tag="JNZ05_003700"
     CDS             973..1629
                     /locus_tag="JNZ05_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAY8566744.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKSGITPDGLTLVGFAIGVLALP
                     FLALGWYLAAPVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWFAWFAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ
                     "
     gene            complement(1638..2945)
                     /locus_tag="JNZ05_003701"
     CDS             complement(1638..2945)
                     /locus_tag="JNZ05_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HAY8566745.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDTLSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPVGGWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPGSSK"
     gene            complement(3012..3665)
                     /locus_tag="JNZ05_003702"
     CDS             complement(3012..3665)
                     /locus_tag="JNZ05_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY8566746.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3665..5199)
                     /locus_tag="JNZ05_003703"
                     /pseudo
     CDS             complement(3665..5199)
                     /locus_tag="JNZ05_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(5172..>6019)
                     /locus_tag="JNZ05_003704"
     CDS             complement(5172..>6019)
                     /locus_tag="JNZ05_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY8566747.1"
                     /translation="KEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGA
                     QLTFIARRDVTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPA
                     ALKEAPDDATFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTF
                     NPAHAQQKIASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDA
                     QLRKADPVVWGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLR
                     RYGTH"
BASE COUNT         1414 a         1616 c         1597 g         1392 t
ORIGIN      
        1 tccatagata taaaaacctt atatattaat acgattgtgc ttatgttgtt tatgtcatgt
       61 gcttttgcag ttttcgacta taccatcaag aatgtgacgg taagcaagaa aaaaatgacc
      121 caggaaagca aattaataac gagagtaatc tcataaccaa atgcgataac tcagacgcta
      181 aagttttgcc acccggctac cgttatcagt agcagagaca ataacgttaa ggagttccct
      241 gtgagtcgag cattgttcgc cgtagtatta gcatttccat taattgcact ggctaacccg
      301 cattatcgtc cggacgtgga ggtaaacgta ccgccagaag tgttcagttc aggcgggcag
      361 tcagcgcagc cctgtactca gtgctgcgtt tatcaggatc aaaattattc tgaaggggcg
      421 gtaattaaag ccgaaggaat actgctgcaa tgccagcgtg atgacaaaac ccttagcact
      481 aatccgctgc tctggcggcg cgtaaagcca taaacgcctc taataatgga atgtcagcag
      541 gggccagcgg atattgcagc gcctcttcag gtgagcacca gaccagcgcc tgatgttcat
      601 gtgcctgtaa cgtcccgtgg aagtcgggta cgtgccaggc atgaagatgg ataatccggc
      661 ccgaaacttc tcgctgatgg ctggcaacat actcacccac agttgcttcg atgtccaatt
      721 cttcgtttaa ctcacgcacc agcgcctgct gctggctttc atccagctcg actttacctc
      781 cggcaaactc ccataatccc gcctgatcgc tctgggcggg gcgttgcgcg agtaaaattt
      841 tgccatcacg ttcaatgatg gcggcaacaa cttcaatcat tttcataaat tacacacggg
      901 cagataagcg aaaaatgcca taatatccgc actgttgctc aatcagaaag gctttatatt
      961 taaggagtgc ctgtgctaga ccgccatctt catccccgga ttaaaccgtt gttgcatcag
     1021 tgcgtgcggg ttcttgataa atcgggcatt acgccagatg gtttaacatt agtgggattt
     1081 gccatcggcg tgctggctct gccgtttctg gcgctgggct ggtatctggc ggcgccagtc
     1141 gtcattttgt tgaacaggct gcttgatggt ctggacggtg cgctggcgcg gcgcagaggg
     1201 cttaccgacg cgggcggctt tcttgatatt tctctcgatt ttctctttta cgcgctggtg
     1261 ccgtttggct ttattcttgc tgcaccggag caaaatgcgc tggcaggcgg ctggttgttg
     1321 tttgcgttta tcggcacggg cagcagtttt ctcgcctttg ctgcactggc agcaaaacat
     1381 cagattgata accccggtta tgcacataag tcgttttact atctgggggg attaaccgaa
     1441 ggcacagaaa cgatcttact gtttgtgctg ggatgtttat tccctgcatg gtttgcgtgg
     1501 tttgcgtggt ttgcgtggtt tgcgtggttt gcgtggtttg cgtggatctt cggtgcgttg
     1561 tgctggatga cgacctttac gcgggtgtgg agtgggtatc tgacgctgaa gtcgctccag
     1621 cgtcagtaat tgcaatgtta tttgctactg cccgggccgc gttcaccggt tgcaaccgga
     1681 ttttttggat cgctgctcca ttcgtaccag ccgccgtcat acacggaaac attcttccaa
     1741 cccatggcgc gtgcgtacat aaaggtttcg gacgcgcgcc agccggtgcc gcagtagaat
     1801 gaaacttgct gatctggttt gatattccat gctttccaca tagcggtaat atcatcggcg
     1861 ctacgcatgg tgccatccgg gttatggaaa tcttccatat gcgtcgagtc gctaccagcg
     1921 tgtccccaac gtgctccggc tatttcacct tttggtttaa tatagctgta accgctggtc
     1981 gtaccgataa attctggcca cgaacgaatg ctcaccagcg atgcatcctg gcgatgcagc
     2041 agtccacggg cctgttccat atcgagcatc aactgcggtt gtgccgggat cttcacgccg
     2101 aaatctggtt ccgctttcac tttcggtggc gttcctcgct caacaggcag tcccgcgtcg
     2161 gaccaggttt gccagccgcc atccagcaga cgcacatctt tcacgcctgc gtagagcata
     2221 atttgcgcca cacgcgctcc agcgtatacg tcacgcccgt acagaatgac cgtggtgtca
     2281 tggcgaatgc cgtgttttgc cagcatcgct tttagttgtt catcagaaac tttgttccac
     2341 agcggttcgc tttccacttc gttggtatcg atgtagtcag cgccaggaat atggctgata
     2401 aggtaaagct taggagcgcc ccaggccgct tcaatgactt tccagccacc gacaggtttc
     2461 gccgtaacct ctttaccttg ttgcaggtca tgcagccatt gcggataaac cagctgctca
     2521 aagtgcggca gtttttgtag acgggaaggt tcgcttagcg tgtcactcag aatggagata
     2581 tgcgtaaaac ctgctttttg cagtcgcgtt ttgacggcgt cgacatcttt gccattaccg
     2641 tacagcgcca ccggggcatc ggctttcagg ttatgttgct tgatccacgc gttgagctgt
     2701 tcggtgctca ttttgtcgag ccagctagca gagaggttta aggcggcagg ttcatgacca
     2761 gaagggccat ttaaggtttg tggccagccg ttataaaacg cgctggggcg agtatctatc
     2821 gctttgccat tttgttgctg aagttggtca agtgtaagag gcttcgccag ttcagcggcc
     2881 cacgaagaag cgcaagccag ccctaaagcc attgccagcg cggtcatttg agaaacacgt
     2941 ttcatcgaaa atcccgacat tatagttttg agtgcgccat tctgaactga cgcattgaga
     3001 aaaactttgc gttatcgcag tttgttgtaa ttttctgacc actgcgccat atccagaaca
     3061 gaactatcag caggaacatc ctggagatcg tgcgttacct gaacgacggg gatcgccagg
     3121 gcgcgaactt cgctgaacac ccactggcga aaattatcgc gcagagccac atcaagacgg
     3181 ctgaatggct catccaggag taacgctttt ggctgggcga gaagggcgcg tagtagagca
     3241 acgcgcgctc gctgaccgcc agacaaagtg gcaggatcct gatggaaagc tccgtcaagg
     3301 cctgaacgct caagtgcatc attcacggca ttacgtcggg cattcccctt aagtgtcgcc
     3361 ggtagcgcca gcagtaaatt ttgcccgacg ctgaactggt caaataacag tgcatcctga
     3421 aaaagaatgc caatctgacg ctgtgcggtg ggtagcatgt caatccgttg ctcattgagc
     3481 catagctcac ctgtacaaga aaactgtccg gccagtgcac caatcatcca tgaaaacaga
     3541 gtggattttc cacagccaga tggccccatt aacgtgacga tgtcaccttt atcaaccgta
     3601 aagttaacgt ttgtcagcaa gcggctttct ggtaaacgta gcgaaacatt tttcacgcag
     3661 agcattagcg gattccttgt ctgacataac ctacccattt tgcgactaac gcggtcaggg
     3721 caaaaataat aagcggtaac aacagttgcc ataaagcctg ggcggcgaga ataccgttgc
     3781 tgccgccgct gcttaatgcc accgcctcag tggtgagcgt cggaaaacgc cccgcgccca
     3841 gccacagcgt cggcatatac tgcgcaatac tgactgaaaa tcccaccgcg aaggcaatca
     3901 gcacagggcg caacatgagt gggcatttca cgtaaaagaa gattttggcc cgcgaccagc
     3961 ccagtgtttg cgcaatcaaa attaaccgtg aatcaatgcg ctgccaggcg ggttgcagga
     4021 taaacagcat ccacggcatt acccacagca gatgccccca gaccaccgcc gtccagcttc
     4081 catccaggtt cagccatagc gccagcgtgt actggcccgc caccagtggc agagcgggca
     4141 atagaatggg caaccatagc cacaactggc gacgctgtgc cccccattcc agccacagca
     4201 gtaacaggag caaagcgatg aatgttgcta ccagccccat tgtcagactg ttgatgagtg
     4261 cttcactatt gatcgtcgac tgatccgcga ggatcgccag cagaaccaca cacagcacac
     4321 cggttaaggg taaaaaactc gccagcgtag tgcctggtaa taaaggtgtg gcaggcttgc
     4381 gaatgccatc tacgcgggga atagtgcgcc gccagctgcg ccatagcaga tagctcagca
     4441 aaacgtaggc ggcgagtaac agcatcagca gcagactggc gagtgcgcct ttcgtttgtt
     4501 gatccgcgtc gccctgagtt aaccactgcc agctaattac tgccagcgtc ggtggattac
     4561 ctggcccaag aataatcgcc acatccacga ccgaaagcga ccaggcaaca atcgccagca
     4621 tcgccattgc cagcgaaggc gcgacggagg gcaacagcag ccagttcagg cattgccagc
     4681 ggctgtagcc cagcgaatcc agcacaatga cctgctgcaa cagccgtttt tcgctcaata
     4741 ctgccactaa gatccacagc agaaatgcgc tttctttcac tgcaagggtg aggcccagac
     4801 cgatccaaat cggtccattg gcggagtgaa atacgggaaa tagtcatata gcagccctcc
     4861 gtcagcaaag agcagcaggg cgctggtggc aaaagccaca tggggaatgg cgagcagcca
     4921 cggcagacgg gcgcacatac gctgccattt cggcccaggc catagcgcca caatcaccag
     4981 cagggcaatc aacaatgccc cgaccgccac gatggttgtc gacaccagcg ttgccagtaa
     5041 tgcctgcggt aactgtggat cggcaaataa cgcctgccag cgtgtcaacg acaacgcagg
     5101 cgatatcagc gtgagagctg ccgggatcag cggtgcataa atcaccgcca ccatcgccca
     5161 cagcaaaaag attaatgcgt accgtaacgg cgtaaccatt cttgttccag tgcattcacc
     5221 catcctgcgt gcggttcagc cagtaccggc ggcagatcct gcggcattct tgattgcaat
     5281 atttcgcgct gcccgtcagg cagtttttgc ggatcgagaa cagaaggatc gccccagaca
     5341 acgggatctg ctttacgcag ttgcgcatcg ggtgagagca ggaaattggc aactaccttc
     5401 gccgcagcac tggcattcgc gttggctgga atggtgacga aatgcacgtt gccaatcatc
     5461 ccctcgcgaa agccaaaact gtaactgctt gccggcaaat cgccgctggc gattttttgc
     5521 tgcgcatgcg cggggttaaa ggtcagcgac aggcgcaatg tgccggcttt cagcagagca
     5581 tccatccgcg cgggcgaagg cgggaaatcc tttccttcgc gccacaaata cggatgcagt
     5641 gcatcaagat attgccacaa gggcgcagtg acacgggcga aagtcgcatc gtccggcgct
     5701 tcttttaatg cggcgggatc tggcgtcagc ataatcagca actgttcaag aaacgccgtg
     5761 ccggtaaagt ccggtgggcg cggataagta accgtgccgg gattggcttt agcaaactcc
     5821 agtaaggctt gcggcgtttg tggtggctgt ggcgtaacat cgcggcgggc gataaacgtc
     5881 agttgtgcgc cgccccaggg cgattccgca ccttgtgtcg gcactgaaaa atcttcccgc
     5941 accggcagct gtgtgtcgac atagcgccag ttgggcagag tctccgccca gcccgtttgt
     6001 aataaattgg cctctttta
//
LOCUS       DADMEQ010000170         5994 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.172, whole genome shotgun sequence.
ACCESSION   DADMEQ010000170 DADMEQ010000000
VERSION     DADMEQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.172"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..268)
                     /locus_tag="JNZ05_003705"
     CDS             complement(<1..268)
                     /locus_tag="JNZ05_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY8566748.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATE"
     gene            805..2706
                     /locus_tag="JNZ05_003706"
     CDS             805..2706
                     /locus_tag="JNZ05_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAY8566749.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGNVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSFDDSSANLFKLGAENIFLGRKAATKEE
                     AIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVF
                     CQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDE
                     VLELLAGRK"
     gene            2801..3949
                     /gene="mtlD"
                     /locus_tag="JNZ05_003707"
     CDS             2801..3949
                     /gene="mtlD"
                     /locus_tag="JNZ05_003707"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY8566750.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            3949..4536
                     /gene="mtlR"
                     /locus_tag="JNZ05_003708"
     CDS             3949..4536
                     /gene="mtlR"
                     /locus_tag="JNZ05_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor MtlR"
                     /protein_id="HAY8566751.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(4548..4757)
                     /locus_tag="JNZ05_003709"
     CDS             complement(4548..4757)
                     /locus_tag="JNZ05_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566752.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5041..5403
                     /locus_tag="JNZ05_003710"
     CDS             5041..5403
                     /locus_tag="JNZ05_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HAY8566753.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         1564 a         1455 c         1488 g         1487 t
ORIGIN      
        1 gttcagtcgc tttctcctgc gacgcaacat gtgactgcat accccagcca cgctccggtt
       61 gatagagcgt cgcccagatc cacagaaacg gccagataac atgcagagta aacaggctca
      121 cccagcccgc cgtatgaata gcgtcggcat gggggtgatt gcgctttttg gcaatcagat
      181 aagggatgtc atgtatcgcg atgattccat aaaaaatcac cagaaagacg aagatcagca
      241 ctcccaacgc gaaatagttt agaaacatat ctgcctcgga ttcacgttta tcagtgttgt
      301 tttttggctg gcagccagaa gggagtcagg ctgatatttt gacaataatc cgggttcgcg
      361 attctcgcca taacaccaaa gaataatttt tagaggtgat gagttgctta gttacataac
      421 gattgtatga cgaaggcata acatgctgta gatcacatca ggtgaacgcc gtaagaaaat
      481 atcttgtgat tcagatcaca aagcttcaac aagccatcaa aacaaaaatg tgacattact
      541 cacatttaaa tgccattttt agcgaaaatc accgccttgt ttctttttta cacaggcgtt
      601 ttgtgatgag cgtcacgtca attacctctc taccccctat atttatgtga ctgatatcac
      661 acaaaagccc gtcgactgga cagttaaccg attcagtgcc agatttcgca gtatctacaa
      721 ggtccggcta cctctgccgc cacattaaca aaaaacctcg ggcttccagc ctgcgcgaca
      781 gcaaacataa gaaggggtgt ttttatgtca tccgatatta agatcaaagt gcaaagcttt
      841 ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg cgtttatcgc gtggggtatc
      901 atcaccgcgt tatttattcc aacagggtgg ttaccgaacg agacgctggc gaagctggtc
      961 gggccgatga tcacttatct cctaccgctg ctgatcggtt ataccggtgg taagctggta
     1021 ggcggcaaac gtggcggcgt agtcggtgcc attaccacca tgggcgttat cgtcggcgca
     1081 gacatgccga tgttcctcgg ttctatgatt gcaggtccgc tgggcggctg gtgcattaag
     1141 cacttcgacc gctgggtaga cggtaagatc aaatccggtt ttgagatgct ggtgaataac
     1201 ttctccgcag gcatcatcgg gatgatcctc gctattctgg cattcctcgg cattggcccg
     1261 attgttgaag ccctgtccaa aatgctggct gcgggcgtta acttcatggt tgtccatgac
     1321 atgctgccgc tggcgtctat ctttgttgaa ccggcgaaaa tcctgttcct caacaacgcc
     1381 attaaccacg gtatcttctc gccgctgggt attcagcagt cccatgaact gggtaaatca
     1441 atcttcttcc tgattgaagc taacccaggt ccaggtatgg gcgtgctgct ggcgtacatg
     1501 ttctttggtc gtggtagcgc taaacagtct gcgggcggtg cggcaatcat ccacttcctg
     1561 ggtggtatcc acgaaatcta cttcccgtat gtgctgatga atccgcgtct gatcctcgca
     1621 gtcatcctcg gcggtatgac tggcgtgttc acgctgacta tcctgggcgg tggtctggtt
     1681 tctccggcat ctccgggttc tatccttgct gtactggcga tgacaccaaa aggtgcttat
     1741 ttcgctaaca tcgcgggtgt gtgtgcggcg atggctgtct ccttcgttgt ctctgctatt
     1801 ttgctgaaaa ccagcaaagt gaaagaagaa gatgatattg aagcagcaac tcgtcgtatg
     1861 caggacatga aagctgagtc taaaggcaca tctccgctat ctgcgggcaa tgtgactaac
     1921 gacctgagcc acgtacgtaa aatcatcgtt gcctgtgacg ccggtatggg ttcaagtgcg
     1981 atgggcgcag gcgtgctgcg taagaaaatt caggatgcag gtctgtcgca gatttcggtc
     2041 actaacagcg cgatcaacaa cctgccgcca gatgtggacc tcgtcatcac tcaccgtgac
     2101 ctgaccgaac gcgctatgcg ccaggttccg caggcacagc atatttcgct gaccaacttc
     2161 ctcgacagcg gcctgtacac cagcctaacc gaacgtctcg ttgctgccca gcgccatact
     2221 gaaaacgaag tgaaagtgaa agacagcttt gacgattcca gtgctaacct gttcaaactg
     2281 ggcgcggaga acatcttcct cggtcgcaaa gcagcaacca aagaagaagc gattcgtttt
     2341 gctggcgagc agctggtgaa aggcggttac gttgagccgg aatacgttca ggcgatgctg
     2401 gatcgtgaaa aactgacccc gacttatctg ggtgagtcta tcgcggtgcc acacggtacg
     2461 gttgaagcga aagatcgcgt actgaaaacg ggcgtggtgt tctgccagta cccggaaggc
     2521 gtgcgcttcg gtgaagaaga agatgacatt gcccgtctgg tgattggtat tgcagcccgt
     2581 aacaacgagc acattcaggt tatcaccagc ctgaccaatg cactggatga tgagtccgtc
     2641 atcgagcgtc tggcacacac caccagcgtg gatgaagtgc tggaactgct ggcaggtcgt
     2701 aagtaatcca atcctaccct ctcctcacgg agagggtttg ggtgagggaa aagcctcacc
     2761 ccagccctct cgggtaaaaa cattgatgaa ggttaatact atgaaagcat tacattttgg
     2821 cgcaggtaat atcggtcgtg gctttatcgg taaactgctg gcagacgcgg gtatccaact
     2881 gacgtttgcc gatgtcaatc aggtggtact tgatgccctg aatgcccgtc atagctatca
     2941 ggttcatgtg gtcggtgaaa ccgagcaggt agataccgtt tccggcgtca atgctgtcag
     3001 cagcattggt gatgatgtcg ttgatctgat tgctcaggtc gatttagtca ctaccgccgt
     3061 tggcccggtt gtgctggaac gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa
     3121 agaacaaggt aatgaatccc cgctgaacat catcgcctgt gaaaacatgg tacgcggcac
     3181 cacgcagctg aaaggtcatg tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga
     3241 agagcacgtt ggctttgtcg attccgccgt tgaccgcatc gtaccgcctt cggcttcggc
     3301 aactaacgat ccgctggaag tgacggtaga aactttcagc gaatggattg tcgataaaac
     3361 gcagttcaaa ggcgcactgc cgaacatccc aggcatggag ttaaccgaca acctgatggc
     3421 atttgtcgaa cgtaaactct tcaccctgaa cacgggtcat gctataaccg cgtacctcgg
     3481 aaaactggcc ggtcatcaga ccattcgtga cgctattctc gacgagaaaa tccgcgcggt
     3541 ggtaaaaggt gcgatggaag aaagtggtgc ggtactgatc aagcgctacg gctttgacgc
     3601 agacaaacat gcggcgtaca tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa
     3661 agatgatgta gagcgcgtag gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct
     3721 gatcaagcca ctgctcggta cgctggaata cagccttccg cacaagaatc tgattcaggg
     3781 gattgctggt gcaatgcact tccgcagtga agatgatccg caggctcagg aactggcagc
     3841 actgatcgct gacaaaggtc cgcaggcggc gctggcacag atttccggtc ttgatgccaa
     3901 cagcgaggtt gtatccgagg cggtaaccgc ttataaagca atgcaataat ggtggaccag
     3961 gcgcaggaca ccctgcgccc gaataacaga ttgtcagata tgcaggcaac aatggaacaa
     4021 acccaggcct ttgaaaaccg tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc
     4081 tttctgatca ccgccgtcga gcttttgacc gaggcggtaa atcttctggt gcttcaggta
     4141 ttccgcaaag acgattacgc ggtgaagtat gctgtagaac cgttactcga cggcgacggt
     4201 ccgctgggcg atctttctgt gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc
     4261 caggaatacg aagatgcgga actgctgatg gcattgcgtg aagagctaaa tcacgacggc
     4321 aacgagtacg cctttaccga cgacgaaatc cttggaccct ttggtgaact gcactgcgtg
     4381 gcggcgttac caccgccgcc acagtttgaa ccagcagact ccagtttgta tgcaatgcaa
     4441 attcagcgct atcaacaggc tgtgcgatca acaatggtcc tttcactgac tgagctgatt
     4501 tccaaaatca gcttaaaaaa agcctttcaa aagtaagcaa cgtctgctta ctgcccctct
     4561 acctgcttcg gccgataaag ccgacgataa tactccagac gttgtagata taacggcacg
     4621 ctttcatcgg gtattccgga cggaatcgcg ttacggggag gaagtttttt cagatactcc
     4681 cggaacgcct ggcttgatgc catgaaatct acggctttat cgataagaag cggaacgttt
     4741 tcacctaatt tgcccatgat gatatctccg tattaccccg cgccgggaat gcgcggccgc
     4801 caattttagt ttaggttccc ggtaaagatg acattaagga aaacgtgctg aatcctcaat
     4861 aatcatccgc tgctgtttat tatctttttc tatagatttc ttaattaatc aacgaattgc
     4921 gtagtttttg agcatgttaa tgattacgtt gcagaactat tacaatgtca tcagttgtga
     4981 acaaagcacc tggtcgcgca tactaggggc tataaattta tctttatcag aagccatcac
     5041 atgaaagaag tcgaaaaaaa cgaaatcaaa cgtctcagcg atcgcctgga cgccatccgc
     5101 caccagcagg ccgatctgtc gctggttgaa gccgcagaca aatatgccga gctggaaaaa
     5161 gagaaagcca cgctggaagc agaaattgct cgcctgcgtg aggttcatag ccaaaaactg
     5221 agtaaagaag cacagaagct gatgaagatg ccgttccagc gcgcaattac caaaaaagag
     5281 caggctgata tgggcaagct gaagaaaagt gttcgcggac tggtggttgt gcacccaatg
     5341 accgcgctgg ggcgcgaaat gggcctggaa gagatgactg gattttctaa gactacgttt
     5401 taagcccctc agacgactgg ttcgatcgta tcaacccgca tctggcttgc cttagctggt
     5461 caatgcgggg actctcctgt ctactcgcct aattaccaaa caattcactc cctttacttc
     5521 gatttaccgc tggttgatat tctgaaaatg catttttacc gaaatagcca tctataaaca
     5581 ataaaaatat atagaagata tcatttgtta attaacaagc atttacgtct ctttttttcg
     5641 gatttaattg tgaaatttta atcatcatat tcacaaaatc aattatctgt aaaatttttc
     5701 ccagacatgg tttctttcat atttgacatt tattaataat catcattaga atccacgaaa
     5761 aataatatat tcatacaaac acagacatct tttgaaacga atgatacatt tttatgatga
     5821 atgcttaaaa caatatttaa tcgacacaca tcactgtcat taatgtaaag caacatagct
     5881 caccaataat acaatggaca actctaccgg cattgatcgc tggtcggagg aaataaggat
     5941 ttttacatga acttgaagaa gatttttttc agtgctgtca ctgtttcagt tctc
//
LOCUS       DADMEQ010000171         5991 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.173, whole genome shotgun sequence.
ACCESSION   DADMEQ010000171 DADMEQ010000000
VERSION     DADMEQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.173"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(1..104)
                     /locus_tag="JNZ05_003711"
                     /pseudo
     CDS             complement(1..104)
                     /locus_tag="JNZ05_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            228..1499
                     /locus_tag="JNZ05_003712"
                     /pseudo
     CDS             228..1499
                     /locus_tag="JNZ05_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804911.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1512..1958
                     /locus_tag="JNZ05_003713"
     CDS             1512..1958
                     /locus_tag="JNZ05_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAY8566754.1"
                     /translation="MKISGLAFNTRHVNCVEGNTTLYALPKPEVVLRWREQTTDDFRF
                     CFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFL
                     DSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIFIRHV"
     gene            2060..3583
                     /locus_tag="JNZ05_003714"
     CDS             2060..3583
                     /locus_tag="JNZ05_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY8566755.1"
                     /translation="MKRVISYIRFSSERQAKGDSLRRQSKMVNDWLKAHPEYYLDQDL
                     SFRDLGVSGFTGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDVDQASELLR
                     NILRSGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRSHEESLRKSERIQAAWNRKK
                     ELISEGIKISKRCPAWLKLNDDRKSFSVIPEKVETVKRCFQLRLEGKSIWEIVRTLND
                     EGHQTLNQYKAGSFGQTSVQDLLKNRSVIGYFTPKGCEEIADYYPAIIDETTFYKVQQ
                     LSYARYGRKPASDKPMAVNLFKGVIRCSECGHALIITGFSDTRNGIYRCPLRNENRCS
                     AKGLSRKQTDGTLLTLLSRLDRFDSDTTNTIDTLKLHREHISSNIEKLVQLSLEIEDL
                     SAITSKLKQLKNELSQVDADIEKETQRLKSIDVGCLSDCDLTTKAGRIEAQLIIKSAV
                     KEVVLNTAKRRCKVTFHNGKVIDLPITENPSDNVTEAIQSLSEVTERGLIDVDEVII"
     gene            complement(3598..4371)
                     /locus_tag="JNZ05_003715"
     CDS             complement(3598..4371)
                     /locus_tag="JNZ05_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566756.1"
                     /translation="MIYTSQLRYTGKDGVYFHLQGQPDNFLTLSATEQMEYDSPMMVT
                     TQNMQSGQTVTDNVMRSPRTISISGVVVVGESGSILISRDNKLVENFIDTLENWRDQK
                     QIISVICKDGIKIDDSIITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDISAATG
                     KTATTNDGGATSKKNTGNTTTSLGNGMLMCQELYNRSTSELSNEQLQARINCGRNVRV
                     KNGKSTFSDAANEQASKVLNNAGTGSTLKKFSVNPNKRY"
     gene            complement(4535..5944)
                     /locus_tag="JNZ05_003716"
     CDS             complement(4535..5944)
                     /locus_tag="JNZ05_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005112464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAY8566757.1"
                     /translation="MDFVFDKDAGQVKEFTKSLAQTTGLDIAELQSQFAGFGASAKES
                     MGIQGSEELFKNMIGYARLMGRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPG
                     MVQVFAKATGKSEQELFDAMKKGALKSADTLQKVTQELNRQITAKGGWKAISESTQAQ
                     LGNLKNSWNTTLDSIFRGSENGLQDFTRSLTTLLNALGGSGKSLGESLGSLMSDMSHG
                     VDSLTDISYRVRAFFDEVTLAYRGLTDEQKKVVDALGSGLINSLKVLAGVIAVNKVVG
                     VTRSIYGLMTAITRLGQVADTTADNVNRKNGKPSRLNPMNMLLAGSAIYAGYERLAST
                     PERMEAQAKLPELAKESGLKPVFTMDDVKGWFSSAADKIANSQEPMNNFVNNTQSTPP
                     AITGDMVNSNAQLAMTISELSRKVDALTVEAKKPLSVNITGEAIVRPDEPAFFTFNQS
                     ANDQFTQDMLLSSSYPEEE"
BASE COUNT         1717 a         1300 c         1267 g         1707 t
ORIGIN      
        1 gataatcggg aagcgccttc actgatcgaa agttgatttt caagaaccgt tctgacagct
       61 tcggctttga actatttaga gtaacgttgg gtttttctgc tcattattag ctccttctga
      121 tgccattcta tttcaggaag gagtgtccgt taaactcagg ctacctcagt gatttaatat
      181 catgaaatat gtaagctcgt atcgctcttg ggcgtctgga ggtatcgatg gataaagtca
      241 aatatccaac aggcgtcgaa aaccacggcg gcacattacg catctggttt aattttaaag
      301 gtaaacgtgt cagggaaaat cttggtgtcc ctgacactgc caagaacagg aagatcgccg
      361 gggaactgcg gacatcggta tgttttgcca tccgcacagg aagctttgat tatgctgcac
      421 agttccctga ctcccctaac cttcaggctt ttggggtaag taaaaaagaa attacggtga
      481 aggaacttga agaaaagtgg ctggatctga aacgaatgga aatctctgca aatgcattca
      541 atcgctatga atccgttgca agaacgatgg ttccgaaaat tggaggcggc agactggtgt
      601 catcggtaac caaagaggaa ttgctgtata tcaggaaaga tttgctgacc gggtatcaga
      661 attcaacgaa aaacaaagca gcagcaaaag gacggagcgt cgttactgta aattattaca
      721 tgacgacaat cgctggaatg tttcagtttg ctgcagatca cggttactta gaagcaaatc
      781 ccttccaggg aattaagcct cttaaaagag ccagggcaga gccagatccg ttaactcgtg
      841 acgaatttat tcgcctgata gatgcttgcc gacatcagca gacgaaaaac ctgtggtcat
      901 tggctgtgta cacaggaatg cgtcacggtg aactggtctc cctggcctgg gaagatatcg
      961 atatgaaagc aggaactatt actatcaggc gcaattatac gaaactcggt gagttcactc
     1021 tacctaaaac tgaagcaagt acaaacaggg ttgtgcatct tatccagccc gctatcagtg
     1081 tcctgaaaaa tcaggctgaa atgacaagac tgggtaagca gcaccacatc aaggttcaac
     1141 tacgtgaata tggacgttca gtgaatcatg aatgtacttt cgtatttaac ccccaggtgg
     1201 ttagaaaaag caaacaggta ggttttatct acaaggtaga ttccattggc gactcatggg
     1261 aaacagccat taagcgtgcg ggcatcaggc accggaaagc ataccagtca cgacacactt
     1321 atgcgtgctg gtcattatct gccggagcaa acccaagctt cattgccagc tagatgggcc
     1381 atgcaagtgc ccagatggtg ttcaatgtat acggagcatg gatgactgac agtaatgcag
     1441 aacagatcgc aatgctgaat cagaagctgg cagattatgt cccatggtca ccaaagtgac
     1501 acaagagact tattaaaatc agtgggttag ccttcaatac ccgtcatgtt aactgcgtgg
     1561 agggcaacac cacgctttac gccctgccga aacccgaggt tgtcctgcgc tggcgtgaac
     1621 agaccacaga tgacttccgc ttctgtttta agtttccggc gaccatttcg catcaggcag
     1681 cattacgaca ttgcgatgat ttagtgactg aatttttgac ccgcatgtca ccgttggctc
     1741 cgcgcattgg gcaatactgg ctgcaactgc ctgccacatt cggcccacgg gagctgcctg
     1801 cgctttggca ttttctcgat tctctccctg gcgaatttaa ttatggtgtg gaagtccgcc
     1861 atccacagtt tttcgccaaa ggggaagagg aacaaacgct taatcgcggt ttacatcagc
     1921 gcggcgttaa tcgggtgatt ttcatcagac acgtttaaat acaccaccta aagcgataca
     1981 atctaggggt tggtagatga ttcggatgaa gcatcctcat aacaagccca actgctatta
     2041 gtttttatgg tggtaaagaa tgaaacgtgt aatcagctac atcagattca gttctgagcg
     2101 acaagccaaa ggcgactcac tacgcagaca gtctaagatg gttaatgact ggcttaaggc
     2161 tcatcctgag tactaccttg accaagattt atccttccgt gatttggggg tgagtgggtt
     2221 cactggtaag cacttgaaag gtggcttagg tgacttttta accgctatcg agaaagggct
     2281 tgttaaagct ggtgatacgc tcttgataga atctcttgac cgtctgtcac gtcaagatgt
     2341 agaccaagca agtgagctac tcagaaacat tcttcgctct ggtgtagatg tggtcacctt
     2401 gtccgatggt gaacattaca cacgagaatc tcttaaagac ccgttagctc tgattaaaag
     2461 cattctgata atgcagcggt cgcatgagga gagtttgagg aagtctgagc gtatacaagc
     2521 tgcatggaac cgtaaaaaag aactcatctc tgaaggtatc aagatttcta aacgttgtcc
     2581 tgcatggctt aagcttaatg atgaccgtaa aagcttctct gtaatccctg agaaagtgga
     2641 gactgtgaag aggtgctttc agctacggtt agagggtaag tctatctggg agattgtcag
     2701 gactcttaat gatgaaggtc accagacgct taaccaatat aaagcgggtt catttggtca
     2761 aacctcagta caagaccttc tgaaaaatcg ctctgtaatt ggttacttca cacctaaagg
     2821 ctgtgaagag atagctgatt attatcctgc aattatagat gaaacaacat tctacaaagt
     2881 ccagcaacta tcatatgctc gttacggtag aaaacctgct tcagataagc caatggctgt
     2941 gaatcttttc aaaggtgtta tccgttgctc tgaatgtggt catgctctga ttatcacagg
     3001 ctttagcgat acaagaaacg ggatttatcg ctgtcccctt cgtaatgaaa accgttgtag
     3061 tgctaaaggg ctgtcacgta agcaaaccga tggtacattg cttaccctgc tatctcgtct
     3121 tgaccgattt gatagcgata cgacgaatac catcgataca ctgaagttac accgtgaaca
     3181 catctcaagc aatattgaga agctggtaca gctatcactt gaaattgaag atttgtcagc
     3241 tattaccagc aagctaaaac aactaaagaa tgagctatca caggttgatg cggatatcga
     3301 gaaggagaca caacgcctta aatctattga tgtggggtgt ttgtctgatt gtgacttgac
     3361 tacaaaggcg ggaaggattg aagcacaact gataatcaaa agtgctgtta aagaggtcgt
     3421 tttaaatact gctaaacgtc gatgtaaagt gacttttcac aacggtaagg taattgacct
     3481 accaataaca gaaaacccct cagacaacgt tacagaggct atacagtcgc tttctgaagt
     3541 cactgagagg ggtttaatag atgttgatga ggttatcata tagttgagat tctatcgtca
     3601 gtagcgctta ttcggattca ccgaaaactt cttcaaagta ctacctgtac cagcattatt
     3661 aagcaccttt gaagcttgct cattagcggc atctgagaag gtgctttttc cgttcttaac
     3721 tctgacattc ctaccacaat tgatacgggc ttgaagttgc tcattagaaa gttcactcgt
     3781 agagcgatta tatagctcct gacacatcaa cataccgtta cctaagcttg tcgtagtgtt
     3841 ccctgtattc tttttgctgg tagcaccacc atcatttgta gtagctgtct ttccagtagc
     3901 agccgaaata tcagtttgac ccacaatagc tttaaagtta atttcctgaa aagttagctg
     3961 gattcttaga ccatttgaaa taccaacatc tttagaggct ttaaagcttg taataatgga
     4021 gtcatcaatt ttaataccat ctttacagat gaccgaaata atctgcttct ggtcacgcca
     4081 gttttcaaga gtgtcgatga agttctccac cagcttatta tctcttgaga taaggatact
     4141 accggattca ccaaccacaa ccacaccact gattgaaata gttcttggtg aacgcattac
     4201 attatctgtt acagtctgcc ctgattgcat attttgagtg gtcaccatca tagggctgtc
     4261 atattccatc tgttcagttg ctgataaggt taagaaatta tctggctgtc cttgtagatg
     4321 gaagtaaaca ccatctttac cagtgtaacg aagctgacta gtgtaaatca ttaaattctc
     4381 ctttgatttc agagacttag tgtattttaa tcatttttaa ccaagccttt gatatataag
     4441 tgttttctct gttaagcgtg agctacgtaa aaaataattt ttggtgggta tatctgttca
     4501 gaagatttca cgtagcacac ggatatataa gaaatcattc ttcctctgga tagcttgaac
     4561 ttaagagcat atcctgcgtg aactggtcat tagctgattg attgaaagta aagaaagctg
     4621 gttcatccgg tctaacaatt gcttcaccag tgatattaac tgacaagggc ttcttagctt
     4681 ctactgttaa agcatcaacc ttgcgtgata gctcactaat agtcattgca agttgagcat
     4741 tactattaac catgtcaccc gtaatagctg gtggagttga ttgtgtgtta tttacgaagt
     4801 tattcatcgg ttcttgggag ttagcaattt tatcagctgc actactaaac caacctttaa
     4861 catcgtccat agtgaacact ggtttaagtc cagactcttt agctagctct ggtaatttag
     4921 cttgtgcttc cattcgttca ggtgtagaag ctaatctttc ataacctgca tagatagcac
     4981 taccagctaa tagcatattc atagggttta gtcttgatgg tttcccattc tttctattca
     5041 cgttatccgc tgtggtatca gcgacctgac ctaatcttgt gatagcagtc ataagcccgt
     5101 aaatacttct ggttacacca acaactttat tcacagcaat aacaccagcc aagactttta
     5161 aactattaat caagccagaa cctaaagcat caactacttt cttttgctca tcggttaaac
     5221 cacgataagc taatgtcacc tcatcgaaga atgctcttac cctgtagctg atatcagtca
     5281 atgaatcgac accatgcgac atatcgctca tcaagctacc aagagattca ccaagacttt
     5341 taccacttcc accaagagcg tttaaaagcg ttgttaggct tcttgtgaaa tcttgaagac
     5401 cattttcaga acctctgaag atactatcaa gcgtggtatt ccaagagttt ttcaagttcc
     5461 ctagttgtgc ctgtgtactt tctgaaatgg ctttccagcc acctttagcg gtgatttgtc
     5521 tattcagttc ctgagtgacc ttctgaagag tatcggcaga ttttaaagcc cccttcttca
     5581 tggcatcaaa taattcctgt tcactctttc cggttgcttt agcgaatacc tgaaccatac
     5641 caggaacagc ttcagccagt tgccccttaa gctcttcagc cataatctga cccttaccag
     5701 ccatctgtga taatgctgtc agtgctcgct taatctcttc ttcacttctc cccatcagac
     5761 gagcatagcc aatcatattt ttaaaaagtt cttcagaacc ttgaataccc atagactctt
     5821 tagctgaagc accaaaacca gcaaactgtg attgcagttc agcgatatct aaacccgttg
     5881 tttgagctaa tgactttgtg aactctttta cctgacctgc atctttatca aatacgaaat
     5941 caattgaacg ctcagcactt gcacgctgta aaccaatttc cataatcttc t
//
LOCUS       DADMEQ010000172         5980 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.174, whole genome shotgun sequence.
ACCESSION   DADMEQ010000172 DADMEQ010000000
VERSION     DADMEQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.174"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            40..1113
                     /locus_tag="JNZ05_003717"
     CDS             40..1113
                     /locus_tag="JNZ05_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8566758.1"
                     /translation="MNKYIKQWCFSVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            1361..2401
                     /gene="pstS"
                     /locus_tag="JNZ05_003718"
     CDS             1361..2401
                     /gene="pstS"
                     /locus_tag="JNZ05_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAY8566759.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            2488..3447
                     /gene="pstC"
                     /locus_tag="JNZ05_003719"
     CDS             2488..3447
                     /gene="pstC"
                     /locus_tag="JNZ05_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HAY8566760.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            3447..4337
                     /gene="pstA"
                     /locus_tag="JNZ05_003720"
     CDS             3447..4337
                     /gene="pstA"
                     /locus_tag="JNZ05_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAY8566761.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            4429..5202
                     /gene="pstB"
                     /locus_tag="JNZ05_003721"
     CDS             4429..5202
                     /gene="pstB"
                     /locus_tag="JNZ05_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HAY8566762.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            5217..5942
                     /gene="phoU"
                     /locus_tag="JNZ05_003722"
     CDS             5217..5942
                     /gene="phoU"
                     /locus_tag="JNZ05_003722"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAY8566763.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGSDELDKLLAGKDSDK"
BASE COUNT         1486 a         1426 c         1554 g         1514 t
ORIGIN      
        1 taattgaaca gggtgtgaca tgtcattaaa aggattttta tgaacaagta tatcaaacag
       61 tggtgctttt ctgtgtttat gctctcgtta agtagtgtag cacttgcagc tcctaaaggt
      121 atctgtaccc ctgataatgg cgtatttcac agtacgcttg atttctcggg atatcttatt
      181 accgcgaacg aaaataaggt tggaacgaca tttaacacca ctgtaactaa tggatcctca
      241 tatcttggac gctgtcattg tgatactggt aacgtaggag aattccccta tatctattat
      301 acgtcaaaaa taaaccaggc attaacttat gctggggttc actctaatat taattattat
      361 gatcttaatc cgaaccttga tgttgggatt gctatagata ttcttggggt tgggtatgtg
      421 aatgcacctt ttgaatatca tgctaacaac ccgtctggta atacaaagta caattgcaac
      481 cgcattgaac ctttaagtat atccagtggg gctaaagcga tagtatattt ttatatcaag
      541 aagacatttg caggaaaatt gattattcct gaaacaaaga tagtgacatt gtatggaaca
      601 attagccgtg acaccccggt ggattactca cagccgatgg ctgatgttta tattcggggc
      661 gatattacag ctccgcaaag ttgcgaaata aataatttaa agccagtttg ttttgatttt
      721 aaagaaatac cagctgcaga tttttcatct gttgttggaa gtgcggtaac aacacataaa
      781 attactaaaa cagtcactat tgagtgtgaa aacctaggga tactgaatac cgatgatatc
      841 agtacgtctt tttatgctac cgaacccaat acggacaatt caatggtcgt aacttcaaac
      901 tctaacgtgg ggataaaaat ttacgacaaa aataataagg aaatcaaagt gaacggtggt
      961 gagttgccaa cagacatgga taaatcaaca gtgtatggtg aaaaatcagg tagtgtaact
     1021 ttttcagctg ctcctgcgag ccttactgga gctcgcccag ccccggggca atttactgca
     1081 acagcgacga taactgttga gattgtgcgc taataggtct gacaacgatt gttcatcaca
     1141 tcgttgtcat ctcacccatt tctttatcac ttttaaatgc atcatgacaa aaagatggca
     1201 tgtgggattt tcatcttcac atttaaaatc agtaagttat ttggtaattc ctttctgtca
     1261 taaaaatgtc atattcctta tatataactg tcacctgttt gtcctatttt gcttctcgta
     1321 gccaacaaac aatgctttat gaatcctccc aggagacatt atgaaagtta tgcgtaccac
     1381 cgtcgcaact gttgtcgccg cgaccttatc gatgagtgct ttctctgtgt ttgcagaagc
     1441 aagcctgaca ggtgcaggtg caaccttccc tgcgccggtg tatgccaaat gggctgacac
     1501 ttaccagaaa gaaaccggta ataaagttaa ctaccagggt atcggttctt ccggtggcgt
     1561 aaaacagatt atcgctaata ccgttgattt tggtgcctct gacgcgccgc tgtctgacga
     1621 aaaactggct caggaaggtc tgttccagtt cccgaccgtg attggcggcg tggtgctggc
     1681 ggttaacatt ccagggctga agtctggcga actggtgctg gatggtaaaa ccctcggcga
     1741 catctacctg ggcaaaatca agaagtggga tgatgaagcc atcgccaaac tgaatccggg
     1801 tctgaaactg ccttcacaaa acattgctgt agtacgccgc gcagatggct ccgggacttc
     1861 cttcgtcttc accagctacc tggcgaaagt gaacgaagag tggaaaaaca acgttggtac
     1921 tggctctacc gtaaaatggc cgatcggtct gggcggtaaa ggtaacgacg gtatcgccgc
     1981 gttcgttcag cgtctgccgg gtgcaattgg ttatgttgaa tatgcttacg cgaagcagaa
     2041 caacctggcg tacaccaaac tgatctccgc tgatggtaaa ccggttagtc cgaccgaaga
     2101 aaacttcgct aatgcagcaa aaggtgcaga ctggagcaaa accttcgctc aggatctgac
     2161 caaccagaaa ggcgaagatg catggcctat tacctctacc acgttcattc tgatccacaa
     2221 agatcagaag aaaccagaac aaggcacaga ggtgctgaaa ttcttcgact gggcgtacaa
     2281 aaccggggct aaacaggcga acgacctgga ttacgctagc ctgccggata gtgtagttga
     2341 acaggttcgc gctgcgtgga agaccaatat taaagacagt agcggtaagc cgctgtacta
     2401 ataaaactcc aggccgggta cggtgtttta cgccgcatcc ggcattacaa aatgactttg
     2461 taaacgcgtt taactgaaga gtaacttatg gctgcaacca agcccgcttt taacccaccg
     2521 ggtaaaaagg gcgacataat tttcagcgtg ctggtaaaac tggcggcgct gattgtgcta
     2581 ttgatgttgg gtggcattat tgtctctctg atcatctcct cctggccgag cattcagaaa
     2641 tttggtctgg ctttcctatg gaccaaagag tgggatgcac cgaacgatat ctacggggcg
     2701 ctggtgccga tctacggtac gttggtgact tcgtttatcg cgctgctgat cgccgtcccg
     2761 gtgagtttcg gtatcgccct gttcctgact gagcttgcgc ctggctggct gaaacgcccg
     2821 ctgggtatcg ccattgagct gctggcagcc attccaagta tcgtttacgg catgtggggc
     2881 ctgtttatct ttgcgccgct gttcgccgtt tactttcagg agccggtcgg caatatcatg
     2941 tcgaatatcc cgattgttgg cgcgctgttc tctggccccg catttggtat cggtatcctc
     3001 gcggcaggcg tgatcctcgc catcatgatt attccgtaca ttgcggcggt aatgcgtgat
     3061 gtgttcgaac aaaccccggt gatgatgaaa gagtcggcct acggtattgg ctgcaccacc
     3121 tgggaagtta tctggcgtat cgttcttccg ttcaccaaaa atggtgttat cggcggcatc
     3181 atgctggggc tgggccgcgc gctcggtgaa accatggcgg tgacctttat catcggtaac
     3241 acctaccagc tcgacagcgc ctcgctgtac atgccgggca atagtatcac ctctgcgctg
     3301 gcgaacgaat ttgcggaagc ggaatccggt ctgcacgttg ccgcactgat ggaactgggc
     3361 ctgatcctgt ttgtgattac cttcatcgtc ctcgccgcat cgaagtttat gattatgcgc
     3421 ctggctaaga atgagggggc acgctaatgg ctatggttga aatgcaaacc actgcggcgc
     3481 tggctgaatc tcgccgcaaa atgcaggcgc gtcgccgcct caaaaaccgt attgcgctga
     3541 cgctctcgat ggcgacgatg gccttcggcc tgttctggct gatctggatt ttaatgtcca
     3601 ccatcactcg cggtatcgac ggtatgtcgc tggcgctgtt cactgaaatg acgccgccgc
     3661 ccaatacgga aggtggtggt ctggcgaacg ccctggcggg tagcgggctg ttaattttgt
     3721 gggccacggt attcggtacg ccgctgggca ttatggcggg gatttatctg gcggaatatg
     3781 gtcgtaaatc ctggctggca gaagtgattc gcttcattaa cgacattctg ctctccgcgc
     3841 cgtcgattgt ggttggtctg tttgtctaca ccatcgtggt ggcacagatg gagcacttct
     3901 ccggctgggc gggcgtaatt gcgctggcgc tgttgcaggt gccgatcgtt atccgcacca
     3961 ccgagaacat gctgaaactg gtgccggaca gcctgcgtga agcggcttat gcgctgggta
     4021 caccgaagtg gaagatgatc tccgcgatta cgctgaaagc gtcggtgtcc gggattatga
     4081 ccggtatcct gctggcgatt gcccgtattg ccggtgaaac cgcgccgctg ctgtttaccg
     4141 cgctctccaa ccagttctgg agcacggaca tgatgcagcc gatcgccaac ctgccggtga
     4201 cgatctttaa gtttgcgatg agcccgtttg cggaatggca acaactggcc tgggccgggg
     4261 tattgatcat taccctgtgc gtcctgctgc tgaacattct ggcgcgcgtt gtttttgcga
     4321 agaataaaca cggttaacac tttgccggat gcggcgtgaa cgccttaccc ggccctacgg
     4381 taagcctgat tagcgaaaag catcaggtac gatgaggaaa agattgcaat gagtatggtt
     4441 gaaactgccc cgagtaaaat tcaggttcgt aatttgaact tctactacgg caaattccat
     4501 gccctgaaaa acatcaacct ggatatcgcc aaaaaccagg ttacggcgtt tatcgggccg
     4561 tccggctgcg gtaaatcgac gctgctgcgt accttcaaca aaatgtttga actgtacccg
     4621 gagcagcgtg cggaaggtga aattctgctt gatggcgaca acatcctgac caactctcag
     4681 gatatcgccc tgctgcgtgc gaaagtgggc atggtgttcc agaaaccgac gccgttcccg
     4741 atgtccatct acgacaacat cgcctttggc gttcgtctgt ttgagaagct ctcccgtgcc
     4801 gacatggacg agcgcgtgca gtgggcattg accaaagccg cattgtggaa cgaaaccaaa
     4861 gataaattgc accagagcgg ttactctctc tctggtggtc agcaacagcg tctgtgtatt
     4921 gcgcgtggta tcgccattcg cccggaagtg ctgctgctcg acgaaccgtg ttcggcgctc
     4981 gaccccatct ctaccgggcg tattgaagag ctgatcaccg aactgaagca ggattacacc
     5041 gtggtgatcg tcacccacaa catgcagcag gctgcgcgtt gttccgacca cacggcattt
     5101 atgtacctgg gcgaattgat tgagttcagc aacacggacg atctgttcac caagccagcg
     5161 aagaaacaaa cagaagacta catcaccggt cgttacggtt gattcaggag tgcgttatgg
     5221 acagtctcaa tcttaataaa catatttccg gccagttcaa cgccgaactg gaaagtatcc
     5281 gcacgcaggt gatgaccatg ggcggcatgg tggagcagca gctttctgat gcaatcaccg
     5341 cgatgcataa ccaggacagc gatctggcga agcgcgtgat cgaaggcgac aagaacgtca
     5401 acatgatgga agtggcgatc gatgaagcct gcgtgcgcat tatcgccaaa cgtcagccga
     5461 cggcgagcga cctgcgactg gttatggtga tcagtaaaac cattgccgag ctggagcgta
     5521 ttggcgacgt ggcggacaaa atctgccgta ctgcgctgga gaaattctcc cagcagcatc
     5581 agccgttgct ggtaagtctg gagtcgctgg gccgtcatac catccagatg ctgcacgacg
     5641 tgctggacgc gttcgcgcgg atggacattg acgaagcggt acgtatttat cgtgaagata
     5701 aaaaagtcga tcaggaatac gaaggtattg ttcgtcaact gatgacctac atgatggaag
     5761 attcgcgcac tattccgagc gtacttaccg cgctgttctg tgcgcgttca atcgaacgta
     5821 tcggcgaccg ctgccagaat atttgtgagt ttatcttcta ctacgtgaag ggtcaggatt
     5881 tccgtcatgt aggcagtgat gagctggata aactgctggc gggaaaagat agcgacaaat
     5941 aattcaccag acaaatccca ataacttaat tattgggatt
//
LOCUS       DADMEQ010000173         5940 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.175, whole genome shotgun sequence.
ACCESSION   DADMEQ010000173 DADMEQ010000000
VERSION     DADMEQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.175"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            63..581
                     /gene="mddA"
                     /locus_tag="JNZ05_003723"
     CDS             63..581
                     /gene="mddA"
                     /locus_tag="JNZ05_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HAY8566764.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            578..1027
                     /locus_tag="JNZ05_003724"
     CDS             578..1027
                     /locus_tag="JNZ05_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY8566765.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1028..1260)
                     /locus_tag="JNZ05_003725"
                     /pseudo
     CDS             complement(1028..1260)
                     /locus_tag="JNZ05_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
     gene            complement(1346..1519)
                     /locus_tag="JNZ05_003726"
     CDS             complement(1346..1519)
                     /locus_tag="JNZ05_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HAY8566766.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1714..1809
                     /gene="yncL"
                     /locus_tag="JNZ05_003727"
     CDS             1714..1809
                     /gene="yncL"
                     /locus_tag="JNZ05_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HAY8566767.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2211..3020)
                     /locus_tag="JNZ05_003728"
     CDS             complement(2211..3020)
                     /locus_tag="JNZ05_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HAY8566768.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3230..4655)
                     /gene="patD"
                     /locus_tag="JNZ05_003729"
                     /pseudo
     CDS             complement(3230..4655)
                     /gene="patD"
                     /locus_tag="JNZ05_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
     gene            complement(4677..5471)
                     /locus_tag="JNZ05_003730"
     CDS             complement(4677..5471)
                     /locus_tag="JNZ05_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY8566769.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5461..5808)
                     /locus_tag="JNZ05_003731"
                     /pseudo
     CDS             complement(5461..5808)
                     /locus_tag="JNZ05_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT         1555 a         1507 c         1320 g         1558 t
ORIGIN      
        1 tgcttcgggc tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag
       61 acatgtccat ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc
      121 acgccgtgtt gtatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca
      181 ttgcctggtt tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg
      241 gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata
      301 ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat
      361 tgttaagccg attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga
      421 tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg
      481 cacaaatgcc gcaggtaggc actaaatttg gtcgttggct ggatctgaca tttatgcagt
      541 tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc
      601 ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg tgcgcatcac
      661 ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct
      721 ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag
      781 cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat
      841 cagcggatat cagaatgtgg gagcggcgac aactattgcg gtactggtcg ccagccagct
      901 gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt
      961 cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc
     1021 attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggccgctt ccatatcttc
     1081 tttatacttg cgaccgtgat gagcaatagc ggtgcgcaaa cgctgctgct gggtgtagcg
     1141 atcttcccga cttaactccg cgtcatcgct cagggcgatt aataattcgt tcatatgggc
     1201 aatgacgctc tcttcaatcg cggcatccac gcgcgcgatg acttcatcca gatgcgacat
     1261 actttctcct tagccacttg tgaaatttat cgattaatcc aaatgatata agtctgcgtg
     1321 gtgccgtcaa gccagtgagt aagatttaaa acagtgaaaa caacagcgcc accgggaaac
     1381 tcatcggcca tgtggccccc accagaaagg cgcttaagaa acggatgcgt ttcttatcgt
     1441 gagaaagaaa ccaggtgata agaaatgcga ttgctgccat aaccgcataa aaaacaagca
     1501 tatgttgata gagagacatg tacgggcgtc cagccatatg aataaaacgc gcgcaaatat
     1561 gcgccatttt ttgacgtgca tcaagttttt tcaccagttt gagaaaaaat catcaaacag
     1621 caacttgccc ttatccatct tcagaatgag tattattgcg gattttctta acccgtacta
     1681 tacacatagg ggcaatgata aaaggtggca aaaatgaatg tttccagtag aactgtagta
     1741 ctgataaatg tctttgctgc tgttggtttg tttactctta tctctatgag atttggctgg
     1801 tttatttgat gtagtgattt tgcacgcagc acgtactgtc agagaaatca actctctgac
     1861 agtacgcaag atcttctcat actctccaag tttgcaacat tatccttaat atttacttct
     1921 ttttgctggc atctacattg aaatgagtag aaaacatccc cctactgtcg tctatacaaa
     1981 cagcttactg attcaaacgg aatcttgttt tcacattttt ctatcacgaa tactttatac
     2041 gcgcgggagt ctataaatgc tttatacttt caaggaataa cgtaaatata aaaagatggc
     2101 gtaaagtata tgcatggcag gatgtttgca acctccttga tgacgctgcc ccattgaaat
     2161 tgcaaacccc gccctggtta tcttgcccat gtatcatgca aaacagataa ttataggcct
     2221 ctgaatacct cagaaagatt gaatttttta tagtccctct cttgaatatt tccatttgat
     2281 atcaatcgat tcatctcttc aacaaaataa tttatatcat cacacttgtt attatccgga
     2341 agatctgagt agttataatt ccaccatttt attctcaaca gttcatcaat aacatttgaa
     2401 tcaaacctaa atctgattat ttttgcagga actcctgcaa ctatggcata tgggggcaca
     2461 tctttggtaa caacggaatt ggcagcaata acagcaccat cacctattgc gatgccacct
     2521 ttcaatacaa catcatttcc tatccagaca tcatttccta taatcggatt ttcaggtttg
     2581 gtaatgaatg gtttcaatgt ccagctacgt tcaaattcac tcatagcaaa cttatcaaat
     2641 tcaccgttat aagtcatcat gtgagtagta aaacgattca aaggatgatt tccacccatt
     2701 atttttacat ttgatgcaat actacaaaat cgacctatac gtatattccc tgaaaaatgg
     2761 caggtcggaa atgaaaaagc gccgacactg gagaaatttt tcacaggcat tgttgaatat
     2821 tcttcagcaa taacattatc ttcaattgtt atcttgtcac cttttgagaa aagaaataaa
     2881 tttctttctt tatacgactt cttcccctta tgatttaaaa agattttatt atccatacaa
     2941 aactcaaagt gttttttcgt ccatctgaag gtaagggttt tatttttatc ttccgtgaat
     3001 ttgtacaatt gaactttcat tgttaatact tctacttata aattattagt ttaataacga
     3061 tactctaaag ccttatgcta atcaactgtg acaggatgaa aaatcgccat caaacattta
     3121 gcctaatttt tacgccagtt attaatgtaa tcattctaat acttaaccgc cattaaactc
     3181 agaacctaaa acgatagtgg ccgtcctata acagccacta acatctaact taatgtttaa
     3241 ccatgacgtg gcggacgacg gtgtaatcct ccagcccata aagtgacata tccttgccgt
     3301 aaccagaaag tttctgccca ccgtgcggca tttcacttac cagcatgaaa tgggtattga
     3361 cccaggtaca accatattgc agccgtgcgc tgacgcgatg cgccctgccc acatctttcg
     3421 tccataccga agatgcaagt ccgtactggc tgtcattcgc ccagttcacc acctgttctt
     3481 cgttgtcgaa gggcgtaaca ctcactactg gaccaaatac ctctttttgc acgatggcat
     3541 cgtcctgtaa tgcgccagcc agcagcgtcg gcgcatagta atagccatta cccttgcgct
     3601 tttcaccgcc agtgatcact ttgatgtgcc ctgtcgcttt cgcctcttct actgccttgc
     3661 tgacgcgttc gagatgcgcc agcgagctta aaggtccaag ctccgtagac tcgtcatccg
     3721 gcgcaccaga ttttaacgtt gccaccgcag gcacccagtt tttccaccag cgtatcgtaa
     3781 atgccttttt gcgcgtagat ccgacaagcc gcagtacaat cctgtccagc attgtaatag
     3841 ccaaatgtac gtacaccttc gaccactgct tcaatatccg catcatcaaa aacaatcact
     3901 ggcgctttgc caccaagttc catatgagta cgcttaatgg acgacgcggt atggctgatg
     3961 atgtgctcgc cggtggcgat agagcccgtc agcgacacca tccgcacttt gggatgaccg
     4021 gtcagcggat cacccaccgt tttgcctctg ccaaacagta tgttaatcac gcctgccggg
     4081 aagatatctt tcgccagctc tgccaacttc aacgcggtca gcggggtaat ttctgatggt
     4141 ttaagcacta cgcagttccc tgccgccagc gccggagcaa gtttccacgc ggccatcatc
     4201 agcggataat tccacggtgc gatagaagcc acgaccccca acggatcgcg acggatcatc
     4261 gaagtatgac cttcaaaata ttcacctgcc gccagaccat tcagacagcg cgccgcaccc
     4321 gcgaaaaagc gaaaaacatc gacaatcgcc gggatttcat cattgaacgc actatgcagc
     4381 ggtttgccac aattacggga ttccagttcg gcaaaaacct gaccattttc ttcgataaca
     4441 tccgccagtt tcagcaggca ttccgcacgc gctttcggtg tggtttgccc ccattcagca
     4501 aatgctgcat ctgccgcacg cacagcagca tcgacctgct ctgcggatgc ctcggcaatc
     4561 tccagtaaaa cgtcccccgt tgccggatta tagacaggct gtttttcccc ttcgccgcta
     4621 accagttctc cgttaatcag taacttatgt tgcatagatt attcctgtat cagtggttat
     4681 tgaccattgt cgccttcgcg ggttagccac caggccccca ggatcggcaa ggttgttacc
     4741 aacataacca gcagtgccac cacgttggtt accggtacat cacgcggtcg cccaagctga
     4801 ttgagcaacc acaacggtaa cgttcgttca tgacctgccg taaaggtcgt aacgatgatt
     4861 tcatcgaacg acaaggcaaa cgccagcatt cctcctgcca gtaacgccga actgagattc
     4921 ggcaacacta cgtagcggaa ggtttgccag ccattggccc caagatccat tgacgcctca
     4981 accagactcc acgaggtgcg gcgaaaacgg gcgatgacat tgttaaacac caccactacg
     5041 caaaaagtcg catgaccgac cacgatggtg aaaaatcccg gctccagatt gatggtttta
     5101 aaggcggtta atagcgccag accagtgaca atgcccggca gcgcaatggg cagcagcaat
     5161 aacagcgaaa tggcgttttt gccgaaaaag tctcgtctcc acagcgcggc agctgccagc
     5221 gtccctaaca ccagcgcaat taatgtcgcc agcgccgcca ctttaagtga cagcgtcacg
     5281 gcatcaagaa tatcactacg ctgtgctgcc acgctaaacc agcgcagcgt caggccctgc
     5341 ggtggaaaac taaacgccgc atcttcagtg ttaaacgcat aggcggcgat tatcaggatg
     5401 ggaaaatgta ggaaaacaac gccgccccag gccgccagtt tgaggaaaaa cggtgcgcgt
     5461 tcagagtgca tcgaacgctc ccagacgttt cacgaacgcc aggtacagtg cgatgagaat
     5521 aatcggcacc agggtgaatg ccgccgccat cggcatattg ccaatcgccc cctgctggga
     5581 ataaaccata ttgccgataa aatatcctgg aggaccaacc agctgcggga cgataaaatc
     5641 gcccagtgtg agtgagaagg taaagataga gccagcggca atacccggga ttgccagcgg
     5701 cagcaccaca tagcgaaagg tttgtcgtgg acgtgcgccg agatcagccg acgcctgcaa
     5761 caatgacggc ggcaaacgct caagcgccgc ctgaacgggc aggatcatcg agtaggcagc
     5821 ctgacggctg cggcttgtca tggtctgcaa ttaccgttat aaaaacaggc aatgtcattg
     5881 tctttcaggt ggttatatgg cccgttcagc taaaccccgt aaacgtaaac ccgcctctca
//
LOCUS       DADMEQ010000174         5809 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.176, whole genome shotgun sequence.
ACCESSION   DADMEQ010000174 DADMEQ010000000
VERSION     DADMEQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.176"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            34..393
                     /locus_tag="JNZ05_003732"
     CDS             34..393
                     /locus_tag="JNZ05_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HAY8566770.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(513..692)
                     /locus_tag="JNZ05_003733"
     CDS             complement(513..692)
                     /locus_tag="JNZ05_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HAY8566771.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            766..2127
                     /gene="pabB"
                     /locus_tag="JNZ05_003734"
     CDS             766..2127
                     /gene="pabB"
                     /locus_tag="JNZ05_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HAY8566772.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2131..2709
                     /locus_tag="JNZ05_003735"
     CDS             2131..2709
                     /locus_tag="JNZ05_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HAY8566773.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            2893..4257
                     /gene="sdaA"
                     /locus_tag="JNZ05_003736"
     CDS             2893..4257
                     /gene="sdaA"
                     /locus_tag="JNZ05_003736"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY8566774.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4388..>5809
                     /locus_tag="JNZ05_003737"
     CDS             4388..>5809
                     /locus_tag="JNZ05_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY8566775.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPEATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAE"
BASE COUNT         1404 a         1454 c         1466 g         1485 t
ORIGIN      
        1 cgtgccatat cgttataatc cctttgatat tagatgcaaa ttaaggtcat atatagcctt
       61 atcgataaca tggttaattt taaggataag aatatgcctg ctgtaataga taaagccctg
      121 gatttcattg gtgccatgga tgtatcagcg ccaacaccaa gttcgatgaa tgaaagcacg
      181 gcgaagggaa tctttaaata tttaaaagaa ctgggagtac ccgcgagtgc cgctgatatt
      241 acggcgcgag ctgaccagga aggctggaac cctgggttca cggaaaaaat ggttggatgg
      301 gcaaaaaaaa tggagacagg tgaacgctct gtgattaaaa atcctgaata cttttcaaca
      361 tatatgcagg aagaactaaa agcactggtc tgagttaaat ttatatcagc ataaatgggt
      421 caggacgctt ttaatcacat ataaaaagcg tcctttttct cacaatcaaa tgatcatcgg
      481 cttctataac gaaatcgata cggattagtg tgttactcgt cttcatcctc aaaacgcgcc
      541 acgattagct cgccggagtg gttggcacgc agctcttctg ccaccagcgc gattgcctgg
      601 ccgctactca tcccttgtgc catcagttcc tggatccgct cgacagcttt ttgctgctgt
      661 tcatgggtga gtgaaggtaa acctgcaaac attgttaact cctgctaaat tgttggcgct
      721 aattatttca tgctacccgg cacatagcca gtagagtcag gactgatgaa gacgttatct
      781 cccgctgtga ttactttacc ctggcgtcag gacgccgctg aattttattt ctcccgctta
      841 agccacctgc cgtgggcgat gcttttacac tccggctatg ccgatcatcc gtatagccgc
      901 tttgatattg tggtcgccga tccgatttgc actttaacca ctttaggtaa agaaaccgtt
      961 gttagtgaaa gcgaaaaacg cacaacgacc actgatgacc cgctacaggt gctccagcag
     1021 gtgctggatc gcgcagacat tcgcccaacg cataacgaag atttgccatt tcagggcggc
     1081 gcactggggt tgtttggcta cgatctgggc cgccgttttg agtcactgcc agaaattgcg
     1141 gaacaagata tcgttctgcc ggatatggca gtgggtatct acgattgggc gctcattgtc
     1201 gaccaccagc gtcatacagt ttctttgctg agtcataatg atgtcaatgc ccgtcgggcc
     1261 tggctggaaa gccagcaatt ctcgccgcag gaagatttca cgctcacttc cgactggcaa
     1321 tccaatatga cccacgagca gtacggcgaa aaatttcgcc aggtacagga atatctgcac
     1381 agcggtgatt gctatcaggt gaatctcgcc cagcgttttc atgcgaccta ttctggcgat
     1441 gaatggcagg cattccttca gcttaatcag gccaaccgcg cgccatttag cgctttttta
     1501 cgtcttgaac agggtgcaat tttaagcctt tcgccagagc ggtttattct ttgtgataat
     1561 agtgaaatcc agacccgccc gattaaaggc acgctaccac gcctgcccga tcctcaggaa
     1621 gatagcaaac aagcagaaaa actggcgaac tcagcgaaag atcgtgccga aaatctgatg
     1681 attgtcgatt taatgcgtaa tgatatcggt cgtgttgccg tagccggttc ggtaaaagta
     1741 ccagagctct tcgtggtgga acccttccct gccgtgcatc atctggtcag cactataacg
     1801 gcgcgactac cagaacagtt acacgccagc gatctgctgc gcgcagcttt tcctggtggc
     1861 tcaataaccg gggctccgaa agtacgggct atggaaatta tcgacgaact ggaaccgcag
     1921 cgacgtaatg cctggtgcgg cagcattggc tatttgagct tttgcggcaa catggatacc
     1981 agcattacta tccgcacgct gactgccatt aacggacaaa tatactgctc tgcgggcggt
     2041 ggaattgtcg ccgatagcca ggaagaagcg gaatatcagg aaacttttga taaagttaat
     2101 aagatattac gccaactgga gaagtaaaac gtggaatacc gtagcctgac gcttgatgat
     2161 tttttatctc gctttcaact tttgcgcccg caaattaacc gggaaaccct aaatcatcgt
     2221 caggctgccg tgttaatccc catcgtccgt cgaccacaac cggggttgtt gctgactcag
     2281 cgttcaattc atctgcgtaa acacgctgga caagtggcat tccctggagg tgcagtcgat
     2341 gacacggacg catcagttat cgccgccgcg ctgcgcgaag ctgaagaaga ggtcgctata
     2401 ccgccttccg ccgttgaagt tatcggcgtg ctgccgcccg tcgatagcgt aactggctat
     2461 caggtaaccc cagtggtcgg cattatcccg cccgatctgc cgtatcgcgc cagtgaagat
     2521 gaagtctcgg cggtgtttga aatgccgctc gcccaggcat tacatctggg tcgttatcac
     2581 cctttagata tctaccgccg tggcgattca catcgggtat ggctgtcctg gtacgaacag
     2641 tattttgtat ggggaatgac cgcaggcata attcgtgagc tggcgctgca aattggtgtg
     2701 aacccctgac tatacttatc tttacatcta caaaacacta cttgagacaa tcatcgcaat
     2761 attagttaaa tcgcggtttt tgattagttt aattcatgtg aatagttaag ccagtcgccg
     2821 cgttccctct tacactatgc gctgttatta gttcgttact ggaagtccag tcaccttgtc
     2881 aggagtatta tcgtgattag tctattcgac atgtttaagg tggggattgg tccctcatct
     2941 tcccataccg tagggcctat gaaggcgggt aaacagttcg tcgatgatct ggtcgaaaaa
     3001 ggcttactgg atagcgttac tcgcgttgcc gtggacgttt atggttcact gtcgctgacg
     3061 ggtaaaggcc accacaccga tatcgccatt attatgggtc ttgcaggtaa cgaacctgcc
     3121 accgtggata tcgacagtat tcccggtttt attcgcgacg taaaagagcg cgaacgtctg
     3181 ctgctggcac agggacggca tgaagtggat ttcccgcgcg acaacgggat gcgttttcat
     3241 aacggcaacc tgccgctgca tgaaaacggt atgcaaatcc acgcctataa cggcgatgaa
     3301 gtcgtctaca gcaaaactta ttattccatc ggcggcggtt ttatcgtcga tgaagaacac
     3361 tttggtcagg atgctgccaa cgaagtgagc gtgccgtatc cgttcaaatc tgccaccgaa
     3421 ctgctcgcgt actgtaatga aaccggctat tcgctgtctg gtctcgctat gcagaacgaa
     3481 ctggcgctgc acagcaagaa agagatcgac gagtatttcg cgcatgtctg gcaaaccatg
     3541 caggcatgta tcgatcgcgg gatgaacacc gaaggtgtac tgccaggccc gctgcgcgtg
     3601 ccacgtcgtg cgtctgccct gcgccggatg ttagtttcca gcgataaact gtctaacgat
     3661 ccgatgaatg tcattgactg ggtaaacatg tttgcgctgg cagttaacga agaaaacgcc
     3721 gccggtggtc gtgtggtaac tgcgccaacc aacggtgcct gcggtatcgt tccggcagtg
     3781 ctggcttact atgaccactt tattgaatcg gtcagcccag acatctatac ccgttacttt
     3841 atggcagcgg gcgcgattgg tgcattgtat aaaatgaacg cctctatttc cggtgcggaa
     3901 gttggttgcc agggcgaagt gggtgttgcc tgttcaatgg ctgctgcggg tcttgcagaa
     3961 ctgctgggcg gtagcccgga acaggtttgc gtggcggcgg aaattggcat ggaacacaac
     4021 cttggtttaa cctgcgaccc ggttgcaggg caggttcagg tgccgtgcat tgagcgtaat
     4081 gccattgcct ctgtgaaggc gattaacgcc gcacggatgg ctctgcgccg caccagtgca
     4141 ccgcgcgtct cgctggataa ggtcatcgaa acgatgtacg aaaccggtaa ggacatgaac
     4201 gccaaatacc gcgaaacctc acgcggtggt ctggcaatca aagtccagtg tgactaatac
     4261 ttcttactcg cccatctgca acggatgggc gaatttatac ccgctttctc gtctgctgta
     4321 atattcccca ctacacttcc actgttgcgt caggcgtttg tcgccatacg cttacagggt
     4381 ggcccgcatg caaaaagcac aacggatcat taaaacctat cgccgtaatc gaatgattgt
     4441 ttgtacgatt tgcgcactcg ttacgctcgc ttcgaccctg agcgtgcgat ttatttcaca
     4501 gcgtaactta aatcaacaac gggtagtaca attcgccaat cacgctgtag aggaattaga
     4561 taaagtactg cttcctctac aggcaggtag cgaagtcttg cttccgctga ttggtctgcc
     4621 ctgctctgtc gcccatttgc cattacgtaa acaggcggca aaactccaaa ctgtgcgatc
     4681 cattggcctg gtgcaagacg gcacacttta ttgctccagc atttttggtt atcgcaatgt
     4741 gcccgtcgtg gacattctgg ctgaacttcc tgcaccgcaa ccacttttac gcctgacgat
     4801 cgaccgtgcc ctgattaaag gcagtccggt tttgattcaa tggacgcctg cagcaggcag
     4861 tagcaatgct ggggtcatag agatgattaa catcgactta ctgacggcaa tgctccttga
     4921 gccacaactg ccgcaaatca gtagcgccag cctgacggtg gacaaacggc atttgctcta
     4981 tggtaatggg ctggtagatt cccttccgca acctgaagac aatgaaaact atcaggtttc
     5041 ttcgcaaagc tttcctttta ccattaacgt aaatggtccg gaggctacgg cgctggcatg
     5101 gcactatctt ccaacacaat taccgctggc ggtgctgcta agtttactgg tgggctatat
     5161 cgcctggctg gcgaccgctt accggatgag cttttcccgc gaaattaatc tgggcctggc
     5221 gcaacatgag ttcgaattgt tctgtcagcc tttgcttaat gcgcgcagcc agcaatgtat
     5281 tggtgtagag attttgctgc gctggaataa tccgcgtcag ggctggattt caccggatgt
     5341 gtttattcct atcgcggaag aacatcattt aattgtgcca ctgacccgct atgtgatggc
     5401 agaaaccatt cgtcagcgcc atgttttccc gatgagtagt cagtttcatg ttggcattaa
     5461 cgtcgcaccc agccattttc gccgtggtgt gctgataaaa gatctcaatc agtactggtt
     5521 tagcgctcac ccgattcagc aactgatcct cgaaatcacc gaacgcgatg ccttactgga
     5581 tgttgattat cggattgccc gcgagctgca tcgtaaaaac gtcaaactgg cgattgatga
     5641 cttcggcacc ggcaacagct cgttttcctg gcttgaaaca ttacgtcctg acgtgctgaa
     5701 aattgataag tcatttaccg cagctatagg ttctgacgcg gttaactcga cggtgaccga
     5761 tatcatcatc gcgctggggc aaagactgaa tattgaactg gtggcggag
//
LOCUS       DADMEQ010000175         5789 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.177, whole genome shotgun sequence.
ACCESSION   DADMEQ010000175 DADMEQ010000000
VERSION     DADMEQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.177"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..920)
                     /gene="rspA"
                     /locus_tag="JNZ05_003738"
     CDS             complement(<1..920)
                     /gene="rspA"
                     /locus_tag="JNZ05_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HAY8566776.1"
                     /translation="MHCERKTYQAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRE
                     LSVASYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKA
                     KAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMK
                     TTYGMSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMH
                     HRLTPIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDC
                     KQLIEEQLIDYIRTTLTHAGGITGMRRIAD"
     gene            962..1303
                     /locus_tag="JNZ05_003739"
     CDS             962..1303
                     /locus_tag="JNZ05_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HAY8566777.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            1369..1898
                     /gene="speG"
                     /locus_tag="JNZ05_003740"
                     /pseudo
     CDS             1369..1898
                     /gene="speG"
                     /locus_tag="JNZ05_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460462.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(1901..2611)
                     /locus_tag="JNZ05_003741"
     CDS             complement(1901..2611)
                     /locus_tag="JNZ05_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HAY8566778.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            2719..3024
                     /locus_tag="JNZ05_003742"
     CDS             2719..3024
                     /locus_tag="JNZ05_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HAY8566779.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            3223..4344
                     /locus_tag="JNZ05_003743"
     CDS             3223..4344
                     /locus_tag="JNZ05_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY8566780.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEVVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTTKPAYIC"
     gene            4378..4701
                     /locus_tag="JNZ05_003744"
     CDS             4378..4701
                     /locus_tag="JNZ05_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY8566781.1"
                     /translation="MTARAIAMLPILTGNVGISGGNSGARESTYTITIERLPVLDNPV
                     KTSISCFSWTDAIDHGPQMTALRDGVRGKDKLDVPIKFIWNYKDLKRLLVRVTRDLSR
                     PEVLH"
     gene            complement(5279..>5789)
                     /locus_tag="JNZ05_003745"
     CDS             complement(5279..>5789)
                     /locus_tag="JNZ05_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566782.1"
                     /translation="DVLESSKLLKTVELPVLTGSLTTKADAIMDALFENTESSFGSEV
                     SDYGIIAHESQLKALSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTAL
                     SFGCFRHDGENITVVLSRDGDSQSHDLEILGKVFVVAEAATTVKLDTGAATAVLPVVK
                     RLKFTKTEA"
BASE COUNT         1509 a         1473 c         1398 g         1409 t
ORIGIN      
        1 aaatcggcaa tccggcgcat accggtaatt ccgcctgcat gggtcagcgt ggtgcggata
       61 taatcgatga gttgctcttc aatcagttgt ttgcagtccc agatgctgtt gaagacttca
      121 cccactgcga tgggtgtgac ggtatgttgg cgaatgagac ggaagcattc ctggttttcc
      181 gctggcgtcg ggtcttccat ccagaacatg cgataatctt caatgctttt accaaagcgc
      241 gccgcttcaa taggcgttaa gcgatggtgc atgtcatgca gcaaatgttc attaaaacca
      301 aacttgttac gtaccgcgtc aaacaatttc ggcatgaaat cgaggtattt ctccgtcgac
      361 cacagctgct cttccggcca ctgtcctttg gttgcgggtt cataagccag acctttacct
      421 ttcgacatgc cgtaggtggt tttcatacca gggattccgc actgcacgcg gatggctttg
      481 aatcccagct cctgatgacg ggcataatca tccagagctt catcaatact gtgaccggtg
      541 gtatggcaat aaaccatcac cccttcacga gacgcgccgc cgagtaactg gtaaagcggc
      601 atgttggcag ctttggcttt aatatcccac agcgccatat caaccgctga aatggccgac
      661 atcgtaaccg gaccgcgacg ccagtaagca cctttataga aaaactgcca gatatcttcg
      721 atacggtgcg catcgcgacc aataagccgc ggacaaagat gatcctgcaa ataagaggcc
      781 acggaaagct cacgtccatt gagggtggca tccccaaggc ccgtaatacc gtcctcagtg
      841 gtgattttta atgtgacgaa attacgcccc ggacaggtaa caaaaacttc agcctggtac
      901 gttttgcgtt cgcagtggat ggtgtagaat cacctttaat cattcataca gggaatgaat
      961 tatgaaaatc actctcagca aacgaatcgg cctgctcgct tttctgctgc cttgcgcact
     1021 ggcattgagc acaactgttc atgccgaaac taacaaactg gtgattgagt ctggcgacag
     1081 tgcacaaagc cgccagcacg ccgctatgga aaaagagcaa tggaatgaca cgcgcaatct
     1141 gcgccagaaa gtgaataaac gcactgaaaa agagtgggat aaagccgacg ccgcttttga
     1201 taaccgcgat aaatgtgagc aaagcgccaa catcaatgcc tactgggagc ccaatacttt
     1261 gcgctgcctg gaccgtcgaa ctggccgcgt tattaccccc taacctgtta ttgatttaag
     1321 gaatgtaagg acacgttatg ccaagcgccc acagttaagc tacgcccgct ggagcgtgaa
     1381 gatttacgct atgtacatca actcgacaat aacgcaagtg tgatgcgtta ctggtttgag
     1441 gaaccctacg aagcctttgt tgaactctct gatctgtatg ataagcgcat attcacgatc
     1501 agagcgaacg gcgctttgtg gtggaatgtg acggcgaaaa agccggtctg gtggagctgg
     1561 tggaaattaa ccatgttcat cgccgggcag aatttcagat aattatctcc ccggagtatc
     1621 aggggaaagg tctggcaacc cgtgccgcca aattagcaat ggactatggc ttcaccgttc
     1681 tcaatctcta taagctgtat ctgatcgtcg ataaagagaa tgaaaaagcg attcacattt
     1741 accgcaagct tggctttacg gttgaaggtg aattgatgca tgagtttttt attaatggtc
     1801 aatatcgtaa tgccattcgc atgtgtatat tctagcatca gtatctggca gagcacaaaa
     1861 caccgggtca gactctcctg aagccgaccg cacaatagca ttaataataa tcgatcgtat
     1921 ttttgatggt gtaaacccgt tcgacggcgg gttttactcc ttcatcaaca atgatcagct
     1981 gacagtccac cggattggcg tgactgtcat attcacagct ctgttttaca ttaccaactc
     2041 gttgattatt tagtaaagta accgctgtgt aatctaattt tttgatcgga tccgttgatg
     2101 gcgtggcgct gacagataat gttttgtcgt tacttttcgt ggttttaccc agcggatacc
     2161 cctgatcatc atagcgatat tccatctgca tttgtttgct gctggcttta atcacgaagc
     2221 cattatcatc ggtttcccaa ctcaccccgg cagaaggtaa ttctgctagc tggcattttc
     2281 cctgtaaacg tactctcttc tccagcgtct cggcatcacg gtaataattg gcgtccagta
     2341 ccagagcgac cacggtatta ttttccagat ccagtaattc gagtgaatca aaacagcctt
     2401 cttccgacaa agtcccagaa acacgtttcg tcacttcacc ttgctcatcc attaatgtct
     2461 gagtgaaatc ttttaccgga ccacgcagcg gatcaaaatc gaattcatta gagaaactgg
     2521 ccatctcagg ggtaaatgaa agtgttacct ctgtgcggtc acatcctgtg aggaatatcg
     2581 cgagtaagca tggtagtaat ttgtatttca caacagtcac cagagagtag agatgattct
     2641 caatcatagt agcaaataca gtactttaca cgttaaatgc tatgcttaaa gaagttatct
     2701 tcgcgtaagg agcttatgat gaaactctca acctgctgtg ccgcgcttct gctcgccctc
     2761 gcctcacccg cggtactcgc cgcgccggga tcctgtgagc gcatacagag cgatatatca
     2821 cagcgcatta tcaataatgg cgtacccgaa agcagcttca cgttaagtat tgtacccaat
     2881 gaccaggttg atcagcctga ttcccaggtc gtcgggcatt gcgctaatga tacgcataaa
     2941 attctctaca accgcaccac cagcggtaac gtctctgctc ccgcgcagtc tacccaggat
     3001 ggcgcgcctg ccgaaccgca gtaacacatt atcgactgaa cgccggatat gacacatccg
     3061 gcgatttgaa catacaacat aatctcacct tattactcat acccttctat tgatgtggat
     3121 taataattct taacccaaaa tgggtagact ccctctattg ttagcgcgct aaatattcaa
     3181 tatataaaca tctatataac gataaagaac agggagtgag ttatgtccaa aaatgaccga
     3241 atggtgggca tcagccgcag aacactcgtt aaatcaaccg cgataggttc tctggcgctg
     3301 gctgcaggcg gtttttcttt accgttcacc ctgcgcagtg cagcggcagc ggtacaacag
     3361 gccagcgaaa aagtgatctg gggttcctgt tccgtcaact gtggtagccg ctgtgcactt
     3421 cgtctacatg ttaaagataa tgaagtgacc tgggtggaaa ctgacaatac cggcagcgat
     3481 gagtacggca accatcaggt acgcgcctgt ttgcgcggtc gctccatccg ccggcgtatt
     3541 aatcatcccg atcgcttgaa ttacccaatg aaacgcgtgg gtaaacgcgg tgaaggcaaa
     3601 ttcgaacgga ttagctggga tgaagccctg gatactatcg ccagtagcct gaaaaaaacc
     3661 gtcgaacagt atggcaatga agttgtatat attcagtact cttcggggat tgttggcggc
     3721 aatatgaccc gctcttcgcc atcagcctcg gcggtcaaac gcctgatgaa ctgctacggc
     3781 ggttcactca atcagtatgg ctcctacagc accgcgcaaa tttcctgtgc catgccctac
     3841 acctacggca gtaatgatgg caacagcacc acggatattg aaaacagcaa gctggtcgtg
     3901 atgtttggta acaacccggc agaaacccgc atgagcggtg gtggcatcac ttatcttctt
     3961 gaaaaagcgc gcgagaaatc gaacgccaaa atgattgtta tcgatccgcg atataccgat
     4021 acggctgccg gtcgtgaaga cgaatggctc cctattcgcc cgggcaccga tgccgcgctg
     4081 gttgcgggta ttgcctgggt attgattaac gaaaatctcg ttgatcaacc ttttctcgat
     4141 aaatactgcg tcggctatga cgaaaaaacc ttacccgcag atgcacccaa aaatggtcac
     4201 tataaagcct atattcttgg tgaaggtgac gataaaacag caaaaacgcc gcagtgggct
     4261 tcgcaaatta ccggtatccc ggtagaccgt atcatcaaac tggcgcgtga aattggcaca
     4321 acaaaacccg cttatatctg ctagggctgg gggccgcaac gccaggcaaa cggcgagctg
     4381 actgcacgcg ctattgctat gttacctatt ttgacgggca atgtcggcat cagcggcgga
     4441 aatagtggcg cgcgtgaatc gacctatacc attacgatag aacgcctacc ggtgctggat
     4501 aatccggtca aaacgtcaat ctcctgcttc agctggacgg atgctatcga tcatggcccg
     4561 caaatgacgg cactccgcga cggtgtccgg ggcaaagata aactggatgt gcccattaag
     4621 ttcatctgga actacaagga cttgaagcgg ttactcgtta gagtcacgag agaccttagt
     4681 aggcctgagg tcctgcattg agtcaagacc ttgtgtcatt ctttgtgcca gacttgtgtc
     4741 attagacggg atgtctagac ggctggtcag gacccccggt ggcctctttg tcaggactca
     4801 gggtggcacc ggggaacatt ggtgtcttaa ttgtattgaa tgaggtctct aacttttggt
     4861 ccaaaactca tcgttagctg ctcgccagct cacagccgaa cccgaactgt aagcacgacc
     4921 accagcacca gcttcagcag gaagacgaca agatagactt tatgcaggtc catgcgccct
     4981 cctgtgtcta ttgtaccact gcactagata actagtacaa aataaagtcc agaccgaagg
     5041 cccagaccat agttaattaa tcacattgat aatccaccac ctgaagtcct gccaacattc
     5101 agtccagcct tacccatgct aggagtctta accggggtgt cgttctgtgt ctccaccact
     5161 ggtgccgcat caggctctac aggaagctcc acagcaggcg cagggaccgg aggtgatacg
     5221 gatgagaagc ccttagcgat ggaccggagg cctttactaa tgctcttacc cattgctgtt
     5281 acgcttcggt cttggtgaac ttcaggcgtt tgactacagg caggactgcg gttgcagcgc
     5341 cagtgtccag cttaactgtc gtagcagctt cagcgacaac gaagacctta ccgaggattt
     5401 ccaagtcgtg ggactgagag tcgccatcac gtgaaaggac gaccgtaata ttctcaccgt
     5461 cgtgacggaa gcagccgaag ctcagggccg tgaagcgttt agccagcatg aacacacctt
     5521 tgtcctcacc gttgtaatac gcgatgtcgg tacccagcat atccacgata gcgtcttcac
     5581 cagagaagcc ctgtttagca gccagacggg acagcgcttt cagttgggac tcatgggcga
     5641 tgatgccgta atcagacact tcagagccga aagaggactc ggtgttctca aacagagcgt
     5701 ccatgatagc gtctgcctta gtggtcaggc ttccagtcag gactggcagc tctacggtct
     5761 tcagcagctt ggaggactcc agaacgtca
//
LOCUS       DADMEQ010000176         5647 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.178, whole genome shotgun sequence.
ACCESSION   DADMEQ010000176 DADMEQ010000000
VERSION     DADMEQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.178"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..1242)
                     /locus_tag="JNZ05_003746"
     CDS             complement(<1..1242)
                     /locus_tag="JNZ05_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAY8566783.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSL"
     gene            complement(1239..2117)
                     /locus_tag="JNZ05_003747"
     CDS             complement(1239..2117)
                     /locus_tag="JNZ05_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY8566784.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(2128..3035)
                     /locus_tag="JNZ05_003748"
                     /pseudo
     CDS             complement(2128..3035)
                     /locus_tag="JNZ05_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(3022..3255)
                     /locus_tag="JNZ05_003749"
     CDS             complement(3022..3255)
                     /locus_tag="JNZ05_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566785.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(3252..4109)
                     /locus_tag="JNZ05_003750"
     CDS             complement(3252..4109)
                     /locus_tag="JNZ05_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAY8566786.1"
                     /translation="MSAQYIDSAISGRYTVSAPYKAGAGIGVLKFNIEHNRAHAVFSC
                     HEHCYAQIMVGRAGASQDAPGSMLTGYANPVRLTTSVIGVPCGEFFEFNIGAVTMTTL
                     PTLSQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRILDCSPEFCARNFAETSILV
                     RQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNQMERSLSGAGAADM
                     SSVAQNARGVYLHLREIHQIWTSQLYPMLKAVESPLAGKLYDRVGDAVFGAALVDSRL
                     NGSDKEVLP"
     gene            4134..4463
                     /locus_tag="JNZ05_003751"
     CDS             4134..4463
                     /locus_tag="JNZ05_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY8566787.1"
                     /translation="MNYTIEYYSEEVRLEVDQLPLSMRARYQHLVERMKVYGSNLGEP
                     HTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKTQKTPPAERKKAETRM
                     KEVKHDW"
     gene            4453..4761
                     /locus_tag="JNZ05_003752"
     CDS             4453..4761
                     /locus_tag="JNZ05_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005286394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8566788.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(4803..4979)
                     /locus_tag="JNZ05_003753"
     CDS             complement(4803..4979)
                     /locus_tag="JNZ05_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HAY8566789.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(4976..5386)
                     /locus_tag="JNZ05_003754"
     CDS             complement(4976..5386)
                     /locus_tag="JNZ05_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HAY8566790.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(5383..>5647)
                     /locus_tag="JNZ05_003755"
     CDS             complement(5383..>5647)
                     /locus_tag="JNZ05_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566791.1"
                     /translation="RQFENWWSKDESQFTGDDELKEFAWVIWQAPRAAIELDIDWPES
                     NDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
BASE COUNT         1348 a         1514 c         1335 g         1450 t
ORIGIN      
        1 cagagaaccg ttacgttgtt tcgtgatatt gatttctgcg atgcccctgg cctgcgtatc
       61 cgggttgtac acttcatccc tgtaaagcat cagaatgatg tctgcatccg cctctatttc
      121 tccggaattt ttcaggtctg agttcatggg acgtttattg ggtctggact ccacaccgcg
      181 ggagagctgg ctcagcgcaa tcaacggaaa accaccggat tttgccaggc ctttaagccc
      241 ctttgagatt tcacccacgg caaggtcatg acgccccgtg gttcgggttt ttatcagccc
      301 gagataatca accaccacca gcgccgtttc cggatattta atcaggtggt gtttcgttgt
      361 tgcgcatatc tcatcaatgg ccaggttcgc ctggtccacc atccagatat tgcgcccggt
      421 catccgcccc accccttgtg agaaacgcgt ccagtcttca tcttcaaagt gagccacaga
      481 tttcaggcgt gatactggca tccctccagc cgcagacacc atacgttcac caatctggat
      541 gttcgccatc tccatggtga acagaagcac accatgcccc tgctcagtca ccttgtcgat
      601 gatgtccagc gcaagttcgg ttttccccat cgaaggacgg gcggcaatga ataccaggtc
      661 tccgggctcc ataccgcctg tttttgcgtc cagttcatca ataccggtca tcaacgtcct
      721 ggatttctcc agcccctgat tccggcattc aacacgctca accacttccg gaagcacatc
      781 atcaatatgt accggctgaa tgacgccctt tcctgtcgac agtgtgacca tcatgttctg
      841 cgcatccttc agggcatctt cagctgcttc acaggtatgc gcatcacgta atttctgcag
      901 cgcctcattc agtgtttttt ctgcatcgcg cagtgcggca ttgcgccgca acgctgcaac
      961 atagtgctcc agtgaagact tcacccaggt tttacgcccg gtatcagtaa tcaccggggc
     1021 aagttccggc atctcattac acaacagcac ggggtcaatc actcctgaaa cacgggcctg
     1081 tctgcagatg cctgtataga tatcctgata cgctcgtaca gaaaaaacgt ccgctggcag
     1141 tgtggccaga atatccatca cttcaggatc tgctccgcgc agaaagaacg cgccgatgac
     1201 agcgccttca aggtcatcgt tacgccatac tggagttgtc atgcagccac acctctgata
     1261 cgagaacggt aactgggcca gttaaacgac aaccagttgc gtcccccgtc tgtgatcctg
     1321 tcggcaattc ggggactgat gaacgcccac aactcttccg gtgagaggtt actgatcaga
     1381 atggtgggca agatactttc gtacctggcg ttgataattt cctgcaaaat agccatttca
     1441 gccgcgctgc caaactgaac gccaacttcg tcgatgatca gcaaatccat tgacgcataa
     1501 cgctcaataa cttcatccgc tgttttttcg ctgtcattcc gccagcaatt tttcacagca
     1561 cgggtaaggc gcatcacgtc ggtgatctcc acactggcca gatagttacg gatgatgtgt
     1621 tttgccatag ccacagccag atgatttttt ccggtaccac aactgccggt cataacaaga
     1681 ctggtaccgt tctccagcat atctggccaa ttctccgcat agcggcgaca ggccgcaaga
     1741 tttctggctg cgccaggatt aacctccaga taattatcaa actcgcagtc ccgaaaacgc
     1801 agggcaattc cggcgttatc agtcagctct tccgccttga tggacgacag ctccatggtc
     1861 aaatcgttgg cctcagcgat taagcagtca gggcagcatg aaattttttc tctgtcctcg
     1921 ccattacgat cgctccacac cagtatatgc gtgtgatatt cgccatgttt ttcgcaatat
     1981 ccgcgacctt cacgcatcag gcaggaacga taaggccatg gcttttcgcc cttctgagca
     2041 aatgcaatct ctgcccgtaa ctcatccatt cgcgcctgta gtcttttttg ttgttcacgc
     2101 aggttaaacg tcatcatcgc tgtcacctca gaatgtcagt ttgtcactgg atttaccgaa
     2161 tttgtcagac atggctccca ggccagccag gacatcgacc tgtcgctgtc gcccacctcc
     2221 gggagcggct ggctgttgcc agaagtcttc gaagtgacga tcgggtccaa agaacgtcgc
     2281 agcctgcttc acgaactgtg tgccggtatt tcctgtggca cgtacccagg cagcatagcg
     2341 tttcacgcca tcaagcatgg cttcaggttt tattccctcc ctgagccggg ctttccaggc
     2401 tttgaaggct gcggattttg agttaccacc agcacgtttt ggatattcct gccaggcctg
     2461 ttcaaattcc ggtgaatatt cctgtcggga agaacgcgct ggtgcagacg cgtcagcgga
     2521 tgcgccaata gtgtttttac tctctgtagt attctctgaa gtaatctctg ttgtattctc
     2581 tgtaagatcg aaattggttt tcccttctcc gcggcgaggg gtttcccgtg tccgcggtga
     2641 aggctttccc tcctccgcga aattgggttt tacagtttcc cgaaaacggg tttccccatt
     2701 tcgggaaact gattgttttc attgataatt tcattaaggc gctcacaatc tatacggtag
     2761 aacattttgt gctcaagacg cttgttggtt tcaaccaaaa tgcctctgga cacaagatgc
     2821 ttacgcgcta cagcctgttg ttcaaatgta agtccggttt cgtgttgtat ctcttcacgc
     2881 gttttatgta cgccttccgc tgcatgtgct ttatcctgcc agtaaaaaat ctgaccaaag
     2941 aaaataacag cgtgcggact tcccatgtat ttaacgagcc cagggtaata agcaaccgga
     3001 tgtccaaaat cgagcagaag atcagacgga cgcatagcca cctcccaggc gtttaaacat
     3061 ttttccgggc tgaaacgcca ccagcggata actcagggta tgagtacgtc cctgaacctg
     3121 gcagacaacc ttctggcttt cggtactgac caggcaaacc cgcagaacgt agccgttgct
     3181 ggtggtgaac cactgcccca cacgggggca atggttgtat cggtgataca ggaaattaac
     3241 gatgtggcgg atcatgggag cacctccttg tcagaaccat tcagcctgga atcaacaagt
     3301 gcagcaccaa aaacagcatc acctacacgg tcgtacagtt tgccagccag cggagattca
     3361 acggccttaa gcattgggta aagctggctt gtccagattt gatggatttc acgcaaatgc
     3421 aggtatacgc ctctggcgtt ttgtgcgaca gatgacatgt cagccgcacc agctcctgat
     3481 aagctcctct ccatctggtt aaaggcattg atgtatgcct ctttgaaccg ggcggcacgt
     3541 ttaccagtga agcccatagc aaggaaggcg aagccgtcgc gggtaatttg gtagcaggga
     3601 agtttgcgag taccgccgtt gggctggcgt accaaaattg atgtctccgc aaaattgcgg
     3661 gcacaaaact ctggagaaca atccaaaatg cggatctttt tcagaacatc gtcatgacgt
     3721 ttagagaaga agttggcaac agccagggat gaagtaacag cctgaccatc aacgatggca
     3781 atttcaggtt gagagagggt tgggagagta gtcatggtga cagcccctat gttgaattca
     3841 aagaactcac cacatgggac gccaatcaca gaggtggtga gacgtacagg gttggcgtaa
     3901 ccggtcaaca tagaacccgg cgcatcttgc gatgcccctg cacgccccac cataatttgg
     3961 gcgtagcaat gctcatgaca cgaaaaaacc gcatgagcgc ggttatgctc tatattgaat
     4021 ttcaggacgc caatcccggc acccgcttta taaggtgcag agacagtgta acgtcccgaa
     4081 attgcagaat caatatattg tgcagatata acgattttgt tatgttttgc tgtatgaact
     4141 acactatcga atactacagt gaagaggtcc gactggaggt cgatcagctt ccattgagta
     4201 tgcgtgccag ataccagcat cttgttgaac gcatgaaggt atacggcagc aatctcggag
     4261 aacctcacac cagcgccttt ggtgacgggc ttttcgaact cagaattaaa ggcaacgatg
     4321 ggatcgcgcg tgttttttac tgcactctga cagggaaacg catcatcatg ctgcatagtt
     4381 ttgtaaagaa aacgcagaaa acaccgccag ccgaacgcaa gaaagctgaa accagaatga
     4441 aggaggttaa gcatgactgg taaacgcact ctccccacca tgacacacga cgaaatggca
     4501 gccaaatgga tggaagaccc ggcttttaaa gcagaatacg acgctatcgc tgacgaattc
     4561 gcactgcttg atgaaatgct ggcagcacgc aaagaagctg gcttaactca ggctgaagtt
     4621 gccgagcgaa tgggaacaaa agcgaccgcg atcaccagaa tggaaagtaa tctcgcatca
     4681 ggtatcagcg gcccatcatt tgccacacta aaaaaattcg cccgcgctac tggaaaaaaa
     4741 ctccagatcc gcttcgttta accacaccgc gccgtcattc tggcggcgca ccggataaat
     4801 agtcatttcc tcgcacgatg tcttagccac cggatatccc acaggtgagc cgtgtagttg
     4861 aaggttttta cgtcagattc ttttgggatt ggcttgcgtt tatttctgga gcgtttcgtt
     4921 ggaaggtatt tgcagttttc gcagatgatg tcggtgaaac ttcgtcgctg tcgcctcatt
     4981 catacctcct gtcggtaaat ctgacaccct gaccaatagc ccaggctgtt gtgtactcga
     5041 tcagacttgc catgcgcttc acgctcatct gcgcgctgct ttcgcgaatg ttgacgcatt
     5101 ctccttcaag gccgggcaaa acatcagctt cctgttttgt tgccactgca tgaccgctga
     5161 tcaacaaaac cttccattgt tctggtttta accatttgcc gcaccattga acctgacgag
     5221 cgatatccgc cagcatcgcg tgaaattttg cgttctggtc aagatttcgc ttgtagtcag
     5281 taatgcggat ggtgactggc ttgtctttat cgagaggagt tgagaggatg gcatttattg
     5341 cggcttgctg ttgttgctta gttcggagga agattgtttg cttcatcgaa aattcttctc
     5401 tttaattcca gcggctctga tagctttcat tactgcaatt accgttttgt ccttcccatc
     5461 ctcatgcccc atcgcataag caccttcttc accatctttc caaaagtcgt cattcgattc
     5521 gggccagtcg atatccagtt caatagctgc tcgcggtgcc tgccatatca cccaggcaaa
     5581 ctcttttaat tcatcgtctc ctgtgaactg gctttcgtct tttgaccacc agttttcaaa
     5641 ctgtcgg
//
LOCUS       DADMEQ010000177         5599 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.179, whole genome shotgun sequence.
ACCESSION   DADMEQ010000177 DADMEQ010000000
VERSION     DADMEQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.179"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..410
                     /locus_tag="JNZ05_003756"
     CDS             <1..410
                     /locus_tag="JNZ05_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY8566792.1"
                     /translation="VRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDAL
                     CYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGID
                     PEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            442..1056
                     /locus_tag="JNZ05_003757"
                     /pseudo
     CDS             442..1056
                     /locus_tag="JNZ05_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="JNZ05_003758"
     CDS             complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="JNZ05_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HAY8566793.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTSGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKTFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYTGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1378 a         1402 c         1330 g         1489 t
ORIGIN      
        1 ccgtcaggca gcagcgaagc aaagcgttac tgacgtcgct gaatgaatgg atggtggaga
       61 agaatggtac gctgtcgaaa aaatccagac tgggcgaagc gttcagctat gtactgaatc
      121 agtgggatgc cctctgttat tacagtgatg acggtctggc ggaggcggac aataacacag
      181 cagaaagagc gcttcgtacc gtctgtctcg ggaaaaagaa ttacatgttc ttcggcagcg
      241 atcatggagg cgatcgcgga gctctgctgt acggactaat cgggagctgt cggttgaacg
      301 gtatcgatcc agaagcgtat ctgcgccata tcctgagcgt actgccggaa tggccttcca
      361 accgagttga cgaactgctg ccatggaacg tagttctcac cgataaataa gcgtcaatac
      421 ggccttccgt tgacgcttac agaactcctg tggcgcgaga gtgaaaaact gcgtcgttat
      481 aaaaaggagg cccggttgcc agttgccaaa acgctaagcg aatacgactt cattcagttg
      541 ccggaactga atggagctca gttccagcaa ctctgtgaaa cgacagactg ggttgatgct
      601 ggagaaaacg ttctgctgtt cggggccagc gggttgggga aaagccatct ggcggcagcg
      661 atcgtggatg gcgtagtagg ccagggctac cgggcccggt tcaacagcgc aggagagctg
      721 ttgcaggaac tacataaagc cagagctcag ttgaaactga atgagctgct actgaaactg
      781 gatcgctacc gggtgatagt ggtggatgat cttggctatg tcaaacgcga cagcgccgaa
      841 acgggtgtgc tgttcgagtt aatagcgcat cgctatgaac gtgggagcct ggtgataacc
      901 agtaaccatc cgttcagcat gtggggcagc atcttcgtgg atgagactat ggcggtggcg
      961 gcagcagacc gactgatcca tcacggatat atgttcgaac tgaaagggga aagctacagg
     1021 aaaaagacag cgaaggcagt aacaagcgtg acttgatgtc gcactgaagg ggcggccagt
     1081 atagttggcg ccagtcggca aaactagttg acgtctaata cctggacggt cacttctgag
     1141 aaaaggcatt tacacagaat cttaaacagg ctcacggagt tctttcagaa aaaggcctgc
     1201 cgaatggcag gcctatccca ttcgtcagaa aacataacgg aagttggcgt ttatcgcatt
     1261 atccacatta tatttcccga acgctgattt ctccagctcc agtcccaaac gcatattatc
     1321 cttaatttcc gcattcatcc ctaccgtcat cagcatcctg ctatcttttt caccttcgaa
     1381 acgtttctct ccggaagcat cctgcagaac cgtttctccg ttcgccagca ggtcgaactg
     1441 ataacccagc ccggctcgag ctgtgatttt ccagtcgtct ccggagaagg ctcttcccac
     1501 gtcaacacca gtacggccaa tcagtgggtt ataatccttg tctttcatgc tcagagccat
     1561 tccccggtct tcccagctaa aagcttttcc tgatacagaa ccgtaaacca gctctatctg
     1621 tggctccacc caggactctt tcgtcaggtg gtaacgataa ccaacttcag caccggtata
     1681 ccaggaatga gagctgtagt cttttgttcc cagtgaggca aaattcgccg tgtgctgatt
     1741 atcatgatgg agatatttac caatcaggtc aaaataagct ccggaattaa agagtgcaga
     1801 ggcatacaga ccgccaccca cggatttgtt ttttccactg aatgcgtggc tgcttgcatt
     1861 gctgtccgta taggtcagca atgcccccgt aaataaatcc acaccgtcca gctcatgctt
     1921 tctgtcgaca ccaatctgaa cgtgagtgta gttatcgctg tagtcaccat ctgccgaacc
     1981 ggtaccattc attatgcgtg cccagacacc ggcatccccc tgagtatccc gcaggtcacc
     2041 catacgtttg ttcaggttat tgacctctgt aaggaaggtc ttataacctg atgccataaa
     2101 gtcttttgcg gctttcgagg ctccggcatc agcggtagtc tgatatcctg tcagcaccca
     2161 ttttgtggca tcatccgttt tttccgtgct gattactggc gtaacgttac tgaaaccaat
     2221 ctgctgcgta ccggcagtga acgttttctc atcactccct ttcggtgcgg taattaacgt
     2281 cacgctcatt tcactggctg gtttgtttaa aaagtcaacc aggacgctat tattactgcc
     2341 tgacagtttg tctgtgacat tcaactggtc agcccggctg ttgttggtat gcatcaggaa
     2401 cgtggtatta tctgcagaaa gctcttttac ttgtaaagtg gcaaagtgtt tcccgtcccc
     2461 cagttcaatt cgcgagccct tagccttcag tgcaccggcc tctgattttc cgtcagagta
     2521 ccacagagca ttgatcatat ttaccgtact ctgtgccccc gtaatactgc ctctcagaac
     2581 agcgttatat cccttcatta aggagctgaa taacggagag tccgtcttcc ctgcatcagc
     2641 atcactgtct cccagattaa tggttgccgc agtggttcct gcatgaatat ccgaactcag
     2701 atagcccata tttttaacag aaaagctggc gttatcttcg aggttaatcc cttctgttgt
     2761 agaaattacg ggtgagtaat acccctgttt ctgcgcagaa ggcatccccg tcagggtcag
     2821 agaagcattt tcttttacat ccacagcccc ggtgctgaaa ataccgctct gagccgtaag
     2881 ttttgctcct tttccgaggg tcaggctgct gttgacaaag ctgccaacac ggtcaaaaac
     2941 agcattctga gaggtcacac tcacggtgct atcataagcc tcaataccac cggtgaatct
     3001 ctccatgatt tgcagggatg atttattttc cagcgtaaca tttccgctgt aatcactctg
     3061 gtcaccaact gtggttgcag tggcactccc ttcaacagag gagaccgcat taccggttcc
     3121 gtcactcctg tcagtaaaga cacggctact tcccagaatg actgtacttc cattggtggc
     3181 gttaatatct ccctgcatct tgccgtttct ggacagatgg aatgttgccg catcgagttt
     3241 cagtttttcc agcgtaaact gacgggtttc ccagtcgctc tggctggaag aagtcgtcgt
     3301 tccagcatga ataactggat gcccctggaa tatcagcgtg ccggacttct tggttatcga
     3361 accttctggc aggttaactg aaccatcaag agccagtaca tccttacctg agagctgcgg
     3421 aatattcaca tccatattgc cgtttagttg gccgtgatac atataaggct ggctgctggc
     3481 ctcaattttt tgttgcttca cagtatcaat ggctttatta cgatcttttc cgacattctg
     3541 ccagacactg ctgttgttca gatcggagaa gaacggggaa tatgaactcg ctttcaggat
     3601 gaaatattgc ttggtagaac tgtcataata taggtctccc ggcgctcctc tccccccaaa
     3661 aatactgaca gtattaaccg gtacattgat gtcactggct tttaatgtct gaaggtcgag
     3721 tgttaccgtt gattttttct cactgttatt ggtaatgatg gccccgtagt cagccgcctg
     3781 aagacgggta aaggtcagat tattaccatt cagttccaga cgtcctccac ggtatcccca
     3841 cgaaatatta tccggattaa cttgctgtga gtcagaaagc acaactgtcg gacgaccgct
     3901 ggcaatacca acagagctga aagcctgtac ctttccgtca gcatccgcct gctggttgag
     3961 aataacggta ccgtccccca ccttgagtcc gcctgcattc acaccggtcc cgtttactgt
     4021 cagggtacct tcgccagttt tatgcaggtt atcaccggca actccgttca cctgccacag
     4081 aacgtgggtt cctttatcgg tgataatccc gccccccatc catgtctggc cgttcagggc
     4141 ggacaccctg taatcgccgg caaactgcag atacccagct ccctgattaa cggaattctg
     4201 cagaacgacc tcgccattat tgccggtgaa caggaggttt tttcctgcgt tcagatcatt
     4261 tcctttcttt ccatgcatat cccaagtcgt gtttccctga gtcagtgtgc cggtgccatt
     4321 agccgcatca tatttccact gcagtactcc ttcaccagag gtgtaggcta tagttttgtc
     4381 aaaatcattt tgcggttgct gaccaaggaa atcctgagtg gtgacgaccc agttatttcc
     4441 atagttagtt cctgaggaaa gcacgccaat cagtacccat tttttttgca gggaatcata
     4501 agcaaacagg ggggagccgc tgtcccccat attcagataa ttagccagag gtccattcgc
     4561 gggattaaaa atgtcaccgg tctgagctgt aatcatctgt ccgttgttat aggagttcag
     4621 cgcgctgacg gttcccccaa caagaaatcc gccattggta tacacttggg tgcgattacc
     4681 atttttgtct ttgacgtact gcatcccccc cccaaggcga tagaattcag taaagcgtcc
     4741 gccggcctga taggcaccgc tcacagcccc gatgtcagac acttctgccg gcgccacctc
     4801 tgtcaccagt ttactcaggc ggcgggtttt aatatcaaga ccgctattat tatttgtacc
     4861 aaccgcagta taggtgtttt tggcgtatcc gaagctcatc gtgtcagaac cgctgacatg
     4921 tttggcggta accacatact gaggggagta aagtgtatgg tctcccggag gcaggctacc
     4981 ggtagtaata gtggcactac tgaaatcagc catcggggct ttatcaagtt taccgaccag
     5041 attgccctgc ttgtcgtaaa tggaaatatt cgtggtcccc gacgtaaact gacctttatt
     5101 ctcggcaaag tcgcggaata tctgataagg gatctctgca ctgaccgttg ctgacagagc
     5161 tggccatgcc gaagaaagtg ataaagctgc aacagacgta agaataaccc gacgtgaaag
     5221 tctgcgatgg cttttgcagg taaccctgcg ggccagttcg gagacagcaa tcaggctttt
     5281 ggttatatgg caatacttaa gataatatat tttgttcata tagaaacatc aacctaattt
     5341 tgacaaagat taattacata ggatgtagat ggagcagtcc gagattctac taatatcaat
     5401 tattaagcat ctgtttttta gttggtgtaa tataattttt attaaggtaa gcggaaaaac
     5461 aaccaaaaaa aacgcaagat gttatttttt tatcattttg agccagtttg gctgggggga
     5521 tgctcttctc ttaagcttct tgctcaactt taaaaatagt tatgccagtt cggtatcgat
     5581 aaggggcagt ttggatatc
//
LOCUS       DADMEQ010000178         5596 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.180, whole genome shotgun sequence.
ACCESSION   DADMEQ010000178 DADMEQ010000000
VERSION     DADMEQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.180"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..219
                     /locus_tag="JNZ05_003759"
     CDS             <1..219
                     /locus_tag="JNZ05_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="nucleator protein that participates in the
                     polymerization of curlin (CsgA) subunits into curli
                     (extracellular fibers from Escherichia and Salmonella spp.
                     that are involved in the colonization of inert surfaces
                     and biofilm formation); part of the curli secretion and
                     assembly protein complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgF"
                     /protein_id="HAY8566794.1"
                     /translation="ALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIVDIANRNGQ
                     LQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            246..1021
                     /locus_tag="JNZ05_003760"
                     /pseudo
     CDS             246..1021
                     /locus_tag="JNZ05_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(1085..1576)
                     /locus_tag="JNZ05_003761"
     CDS             complement(1085..1576)
                     /locus_tag="JNZ05_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAY8566795.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(1678..2232)
                     /locus_tag="JNZ05_003762"
     CDS             complement(1678..2232)
                     /locus_tag="JNZ05_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY8566796.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(2256..2993)
                     /locus_tag="JNZ05_003763"
     CDS             complement(2256..2993)
                     /locus_tag="JNZ05_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAY8566797.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(3048..3986)
                     /gene="ghrA"
                     /locus_tag="JNZ05_003764"
                     /pseudo
     CDS             complement(3048..3986)
                     /gene="ghrA"
                     /locus_tag="JNZ05_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            4220..4307
                     /locus_tag="JNZ05_003765"
     tRNA            4220..4307
                     /locus_tag="JNZ05_003765"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4254..4256,aa:Ser,seq:gga)
     gene            complement(4500..5486)
                     /locus_tag="JNZ05_003766"
     CDS             complement(4500..5486)
                     /locus_tag="JNZ05_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566798.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFLWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
BASE COUNT         1595 a         1417 c         1242 g         1342 t
ORIGIN      
        1 gcgttagata actttactca ggccatccag tcacaaattt taggtgggct actgtcgaat
       61 attaataccg gtaaaccggg ccgcatggtg accaacgatt atattgttga tattgctaac
      121 cgcaatggtc aattgcagtt gaacgtgaca gatcgtaaaa ccggacaaac ctcgaccatc
      181 caggtttcgg gtttacaaaa taactcaacc gatttttaag ccccagcttc ataaggaaaa
      241 taatcatgca gcgcttattt cttttggttg ccgtcatgtt actgagcgga tgcttaaccg
      301 ccccgcctaa agaagccgcc agaccgacat taatgcctcg tgctcagagc tacaaagatt
      361 tgacccatct gccagcgccg acgggtaaga tctttgtttc ggtatacaac attcaggacg
      421 aaaccgggca atttaaaccc tacccggcaa gtaacttctc cactgctgtt ccgcaaagcg
      481 ccagggctta caaaacctgc ttaacgagcg caaggttatt cgtgcggcac aagaaaacgg
      541 cacggttgcc attaataacc gaatcccgct gcaatcttta acggcggcaa atattatggt
      601 tgaaggttcg attattggtt atgaaagcaa cgttaaatcg ggcggtgttg gggcaagata
      661 ttttgggatc ggtgcagaca cgcaatacca gctcgatcag attgccgtga acctgcgtgt
      721 cgtcaatgtg agtaccggtg agatcctctc ctcggtgaac accagtaaga cgatactttc
      781 ctatgaagtt caggccgggg ttttccgctt tattgactac cagcgcttgc tggaagggga
      841 agtgggttac acctcaaacg aacccgttat gctgtgtctg atgtcggcta tcgaaacagg
      901 ggtcattttc ctgattaatg atggtatcga ccgtggtctg tgggatttgc aaaataaagc
      961 agaacggcag aatgacattc tggtgaaata ccgccatatg tcggttccac cggaatcctg
     1021 acagataaaa atgccggaat taataatccg gcatttactg tttaagcgaa agcgtgagtg
     1081 cccctcacgc tttatttttg atttcctgct cacggtgagc cgtctttgca ctacgcgccg
     1141 ccagccacag gccgctgttt ttcattgcgt aaccaaatac cagcccaagc gccagcgaag
     1201 gtaacaccag tttccagtca ccctgcccgg caaacgtcgc gcatgcgcct ataaacgtcc
     1261 ccggaacaaa tgaaagcagc agctgtttgg cctgaataca catcagaaac gcgacaatac
     1321 cggttatgac ataaccgaga atttccagat gtggtgccag tgcactaccg taaataatga
     1381 ccatcgccca caccacgcca ctaagcaggg ttgcagcgga gatcgccagc cctttcagcc
     1441 caccttgcgg gcaagcaaag taggccgtac agcccaggaa gcccgcccag cttagtaagc
     1501 ccagggaaac agccacccat ccccagatac cggagagaat gcctgttgtg attgcaatag
     1561 agagaagtat gttcatgccg cgcatgatag cagaaaacgc ggccatgaat gagatcgagc
     1621 acacatttta tgcaacaaca aattatctgt tgcagttatg agtgacgatg atcacattta
     1681 ctcttcagaa tcttcttcca gctcgtccca cattgcacta atggcatcac gggttagcgg
     1741 tgccatggtg cgccagaaag gcgtggttgc atgggcctcc actttgccga ggaacgcacc
     1801 acaccagggt aacagatact cactgaacag tgtttccagt gcttcgctct catcttccgt
     1861 tgactgatct tccagccagg aagccgcaag cagcaatgtg ccgatgtgat cggctggcgt
     1921 atccgccagt ggcatccctc gttcggaaag aaaagcgcgc acttccgctt ccgttgcgtc
     1981 ctcaacccat gcgctacgat atggcggcac ggcacattca tcgccgataa acaacgcatt
     2041 gtaatcggca gagacttgcg tcatatcaca gcttttctgt aaacgcgtca gtaactcatc
     2101 ctgctccagt ggccagttcg cagccagttt cccctcacga atcagagtaa acagcggcac
     2161 cagtaaagga tcttgcggtt ggcggtaata gagcgaaccc agcacacgac agaggataga
     2221 aaactcgttc attaatatat ttccattact agtaattaaa ggtctgcaaa ttccgcaatc
     2281 ggtgccatac cgcgagattc aaggaagttc agtaagcggc gcggagaaac attcaaaatg
     2341 cgctctggcg gaaaatctac cgcgtcgagg attttaagac actcttcaaa ttcccccatg
     2401 gtaaacgcgg tgtgagaatc cgagcctaat gccacccaac ctccagcatc acgtaccgct
     2461 gcagctaccg cacgacagtt gtcttcactg cccttacgtg agtgtaaaaa tgaggaatta
     2521 ttgatttcca gcgccacctg atgtttcgcg gctgcttcag caacggcttt aacatctatt
     2581 tcatatttcg gatttccggg atggcttatt atatgcacat tgccgctggc gatagtggcg
     2641 atcatcgctt gtgtattggt cgctttgtca tgtggcgcaa aaaccggctc atgaaaaccg
     2701 gcaataatca gatccagcga gtcaaacatt ttaccgctgc agtcaatttc accatcaaca
     2761 tttttaatgt tagcttcaat gccgcgcagg atccctaccc catcaaccac tcgcggccag
     2821 atacgcatgt taatgaagtg ccagtgatgc ggcgcatctt ccatatccgg gccatgatcg
     2881 gtgatcgcaa aaagtttaat gcccttttgt ttggcctggg caatgtaatc actcaatgtg
     2941 ctatatgcat gtgtgctggc aacggtatgc atatgaaggt cgacgggata cataactctc
     3001 tcctgtattc aattcccgcc aaggatagca ggaatcctga tgctttatta gtagccgcgt
     3061 gcgcggtcga cttgcccgca gaccctctcc cctttttcga gctgggcaat agtgcgagaa
     3121 atgtactcca cagcttcagc gggacgggta atcgcggcga catgtggtgt tatcgtcacg
     3181 cgtggatgtt gccagagcgg actttcaggc ggtaagggtt cacgattaaa aacatccagc
     3241 attgcgcctt taactttccc gctatccagc gccgcgagca ggtcatcttc cacaacatga
     3301 acaccacgcg ccaggttgag gagatacgcg ccatcaggta atttttcgag taattgttga
     3361 ttaataatgc cgacggtttc aggggtattc ggtaacaaat taatcaatac ccgacattgg
     3421 ctcagaaatg cagacagttc ttcccgtccg gcaaagcttt gcacgccagg ccacgattta
     3481 cgggttcgac tccagcaacg cagcggaaag cgctaggttt gcagactctg agcaacttta
     3541 cagcccagta cgcctgcgcc caaaatgccg atggtaaaat cttcccgatg atattcaggc
     3601 agcggttgcc aatgcgaact attttgctgg atgcgataat catcaaaacg tcgaaaccaa
     3661 tgcagcacct gactgacagc atattcctgc atttgctcgc ccataccggt atcttccagg
     3721 cgaaaaagtg gaacagaagg gttcagcatt tcagggtgtg cctgtagctt gctcaaaata
     3781 gaatcaacac cggccccgag tgcgaacacc gctttaagat cgcgccctgc cagcatttca
     3841 acaggaggat gccagactaa aacataatca gcagagtcat tatctccgct tttccatgcc
     3901 ctgactcttg cctgaggaat agctttgcgc agtgcctcaa tccaccattg ggtatcgaac
     3961 gttgggtgat aaaagatgat atccatactg actcccgaaa agcgttgtgc gaaatttatt
     4021 cgcacttatc gttatgatct acaaaggcca ccagcataac aaatccgtgg tcggtggcaa
     4081 aaaaagcaga tttcgcttat taaaaccaca cattgattga agtttgaata aacgcgcgat
     4141 tctttcaaaa agtttgttga cctcaggtca tgatttccct aaattagcgc ccgttccagc
     4201 aagacaggaa cgacaatttg gtgaggtgtc cgagtggctg aaggagcacg cctggaaagt
     4261 gtgtatacgg caacgtatcg ggggttcgaa tccccccctc accgccatat ttaaagaaga
     4321 gcgcgtacga aagtacgggc ttttttttcg tatattgtac acaccgggtg cccccgccca
     4381 cgtctgttag ggcgagggga aattgtgcac atcaactgtc tggttactca gtcaacaatc
     4441 aaaatgcgca agatagtaca gaacatatat gcgttatctg gtcacttcct gtaacttcct
     4501 cacttattaa aatgccccca acggaaatac atgtcacatg ccatcataaa atcgttatgc
     4561 cagaggaatc gacaatggaa aaaatagtaa attaagcacg gtacatagca gagtaaaaaa
     4621 taggcttcaa accaaatccg ccattccaca agcgttgcca ataccgacac cagcgtaatc
     4681 cacatcattt caataaccag caatatgaat aaccgaagcc aaagcggctt ggcaaaaaag
     4741 gtcgacaacg tattttcact ggagaaaaac agatcgattt cctgcaaaaa aaacctgccc
     4801 agaaaagcgt accgcttgga gaagcgcaga aaaaacagat taatcgccaa cactacgcca
     4861 caaagaatga gcaatgcaat tatcgattta aacgttaacc actgcccctc tgtcacaaaa
     4921 gaatcactgt tacgcaacgg gctgatagta agcaggttaa caaccgttga gatgcacacc
     4981 gaaagcagca catagcgagt cagactttta tattttactt ttttcgctac tgaaatagcg
     5041 acctgcttga cataactggt gcggatcatc cgaaacagca gaaagatgac caacggccca
     5101 agtaacgaaa tcaggaacag cgcatataac gagggtagaa gctggtatag cccccaactc
     5161 attgcactca acatgattaa cgacagaaat cctgccacca gaggttgtgt aagaaataat
     5221 tctattttgt ggttctgctt tttcttaaac cacaaaaaat cgatattctt cgtgacgcat
     5281 ttataagccc agtacgcctt gagatcaaaa ataaaattat acagcaccag ggccagaatc
     5341 ccactaacta caccaatatc ctctgatgga aaagtaaacc ctgcattttt ccaataaaag
     5401 cctatcagaa taagtccaat agcataaatg tatatcagat ttcgtttatc ctgaaagcga
     5461 ataaaagtta aataatcagg aatcatttga atacctttgc atcactaaat gctttacgac
     5521 aatgcataac tcgttgctga tccacgccag caaaatcatt taacatattc cccaaatagg
     5581 gcttattact tatttc
//
LOCUS       DADMEQ010000179         5577 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.181, whole genome shotgun sequence.
ACCESSION   DADMEQ010000179 DADMEQ010000000
VERSION     DADMEQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.181"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..87)
                     /locus_tag="JNZ05_003767"
     CDS             complement(<1..87)
                     /locus_tag="JNZ05_003767"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY8566799.1"
                     /translation="MALESDERMLPPNIRGYAPQITGIAETNA"
     gene            complement(249..3557)
                     /gene="icsA"
                     /locus_tag="JNZ05_003768"
                     /gene_synonym="virG"
     CDS             complement(249..3557)
                     /gene="icsA"
                     /locus_tag="JNZ05_003768"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HAY8566800.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4086..5288
                     /gene="virA"
                     /locus_tag="JNZ05_003769"
     CDS             4086..5288
                     /gene="virA"
                     /locus_tag="JNZ05_003769"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HAY8566801.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMSEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1730 a         1254 c          935 g         1658 t
ORIGIN      
        1 tgcattagtt tctgcaatac cagtaatctg aggcgcataa ccacgaatat ttggcggtag
       61 cattcgttca tcgctttcta atgcaattcc tgtgtaacgc caagcaacag cgcattctgg
      121 cgcatgatct ggtaaacacg tttggcattg atcgcaggca taccatcatg tgcacaaaaa
      181 cccaccgagc ttttacacgg tgggtcccag agaaatgcag gacatcaaca cgccctgcat
      241 ttttattatc agaaggtata tttcacaccc aaaatacctt gggtatctct gtaactgtta
      301 ttacccaatt gctgagatac gtttccccag atgcttaggt tatctgcaag acgccccccc
      361 acaccagttt tgagttcaat cacattacgg ttgcctatct ggtgatttga catgccattc
      421 ataattacac catattgctt tgagctccat ttccaattca cttccaaaaa tggttcgaac
      481 ttacgaagag tctccttatc gatacaactc tgactaatta aggatgtacg caatcccaat
      541 tttgcttgaa tattattgac cccagaggat gtgaccatag tgccattgtg ttcaatatag
      601 tcgtttgctt ttaccccatg cctagttatc tggacttgtg gattgagata caatgcgttg
      661 tcaatattat tatgagcagt ccagcgtatg gttgggtaga tatagccagc ttccaaagct
      721 cctgttattc ctttgctgga atatttctca ccagttaacc catctccttt gactgtatta
      781 ttaaaccaac tgtactgcaa ccaagcatcc atatatagac ctgttcgttc tttttcatcc
      841 tgaaaccaag aactgtacaa accggcagta taaccgctca cagttcctcg tgaactataa
      901 ctagtatact tattatgagt tttgctatcc tgatatccat aagctcccat aatacccaca
      961 tgcaagccat catgatcagt gaagtttgtc ttcaaaatat ctccaccaat ctgataaacc
     1021 atcctgttag ctgtggtttt taattgtcta tcagctaaat tatgctcatt atgtccacca
     1081 acagtacgaa tccacatggt ggtttcgctg agctgacctg tagtaggatc aacaatagtg
     1141 ctaccgtgtc ggtcatagag agaaggacta aacatcgtgt tcattgctat caggttagca
     1201 atataactgc cagcttcagg gcgccatgtg tgaatactac cttcacccaa attaagtgaa
     1261 gatacaagct gtgaactagc tcggcgacta ctcatttgag tagactcttg attgctcatc
     1321 tgttttgatt cttgattatc catctgacta gttagatacc acttattggt attcagtcca
     1381 gatacagtgc cctgtttcag gcgatagtca taactaccag caacaatacg gcctttctga
     1441 ataaaagcat tcttatcaga agaatctgtc gaaataattt gaacaccttc aagagtttta
     1501 tctccagagc ctccctcatt tgtcactgca acataggtaa taccagacgc atggcctgca
     1561 atttccagcc ggtcagtttt actgttgtca ttgccgagaa ccgcgcccaa agataatatt
     1621 ccgccctttc catgccaatt acctttctga acataggtct gaccgacatt tgacgaacta
     1681 ttattgagaa taacatgacc gtttttctcc agagtcatgt tacctgtgac aatgacgggg
     1741 tcaggcaaaa aaggggcatt aatattattg attagcacag tcccactatt taataaagtg
     1801 ttaacagttt ggttcatgcc tgaaaaattc aaggtagcac ctttattaac aataacacca
     1861 gcggtacgtg tcatagcttc aactgtcccc atttttagaa tgcctgcatt gatgttggtg
     1921 aaaccagagt aggtattttt ttccgccaaa atgagagttc cctcccctga ttttgttagg
     1981 gattttccgt cccagctatt tatattatga tggctgagat tatctgccag aatagtacta
     2041 acagtaagtt cagcgttttc tttcagattg aactctccat gactgtcgcc atctgtatca
     2101 ttccagctca aaccgtagat caatttattc tggtcactga tatagctttt tgtatagtca
     2161 atagcaccag tagtgagtaa cggattgatg atagatatat tagaaacact gaaacctgtt
     2221 atccctttat ctgcacttag cagtttatag tccttctgcg cccagttatt tataatagct
     2281 tccactttta cattgttgcc aaatgacata tttccagaag cttccagact actgattcca
     2341 gctaaattta aagaactgtt ctcaccaaaa gaaacgtcac ctgaaacagt gaatttgtta
     2401 cctgatagtg aaacagttgt acaatcacca gcacataaat caccttcaat agttggtgtg
     2461 gtcccagtgt aatcattgtt gataattttc agaatggatg aattgttaac ctgtacatca
     2521 ccagtaatga cagaaccttc atggagtatt aatgaattgt ttccaccagt gatgtggata
     2581 gcattacctg ctacattttg tgttccatca tcttctttac ctgaaattat atatccgcta
     2641 ttagcaatgg tcatattgct accatttata gcatcccccc aatgtccacc attaccgcct
     2701 aaaatagctc cattgttgat tacagacagg ttatctcctg tgatagcatt accaccgtag
     2761 ccatcataac cataaccgta agcagcacct ccgttgcctc cagcgaacgt gcccttattg
     2821 attacagaga ggctgcttcc tgtgatggca tcacctccat taccgccatc accctcacca
     2881 taattgttac ctccattacc accggaaata gtccctttgt tgattatggg tagattgact
     2941 cctgtgacgg catcacctcc attcccccca tcactatcac tataactatc gccaccatgt
     3001 ccacctgaaa taatttctcc atttattata aacagattgt ctcctgtaac agcctcacca
     3061 ccatcaccgt tatggtcagc accgctaccg ccgctaccac caagaatcat gccttgattg
     3121 attatagaca agtcagatcc ggtaatagag tcaccaccat taccgccaca actattacca
     3181 tcattattat caccagcacc accatgacct gcgctaatca taatatcaga gttaatccgt
     3241 agcttagttc tagatgcatg agaggggttg gaagaagtta tataccacgc atccattcca
     3301 tcttcaccag ctcccaaggg agaaagtcca tcaacaggtg ttaataattt ttcataattg
     3361 tcctctgaaa aatgaagttc ttgagtaccc gaaagaggag tagcaaaagc tattggcccc
     3421 ccgagcaaca gggatgcacc aacaacaaaa ggagaaaaag acaatttgca tgttttttcc
     3481 tttaccgtag gtaattctcc ggcccccccc tgtgaacatt gggtcatatt acaaaaaaat
     3541 ttgtgaattt gattcatgca ctatattatc agtaagtggt tgataaaccc ctgaaaagat
     3601 tccgttttat ccggaataaa gggacgatat atgcaaaaac atattaaaca aagcctcaac
     3661 caaaacatga gagctgtgtt atatatcaat gcaacgggag aaatttacct gtgtcttgct
     3721 ttgaaaaaaa gagggggaaa gctatatgtt cgataataaa ttaataattt tactatgata
     3781 gtctgcttca gaccgcaact tttcagtcct ttctgtacgc ttgcccaaag tttaaccact
     3841 ttgtttttct atacacccaa tggttctatc tgccccttga tgttaatccc caaagccaat
     3901 aacatgaatg tattcatatc acaactttcc taccagactc tccaatgcaa taactgcata
     3961 attgttttta gtggaaatgt atcgttttct taaagagaag aataacattc cattttatta
     4021 tgttcccaat ttaattatat taatatatat tggcattaat aggaaaatac atcaggagaa
     4081 atcaaatgca gacatcaaac ataactaacc atgaaagaaa tgactcatca tggatgtcaa
     4141 ctgtcaaatc aactacagaa gtaagttgga ataaattgag tttttgtgac gttttactga
     4201 aaataataac tttcggcata tattctccac acgaaactct agccgaaaaa tatagcgaaa
     4261 aaaaactaat ggatagtttc tcaccatcac tatcacaaga taaaatggac ggagagtttg
     4321 cccacgcaaa catagatggc atttcaatac ggctatgcct taacaaaggc atatgttcgg
     4381 ttttttatct tgatggagat aaaatacaat ccactcaact ttcatcaaaa gaatataata
     4441 acttgttaag ttcactcccc cccaaacaat tcaacttagg aaaagtacat actattacag
     4501 ctccggtatc agggaatttt aaaactcaca agcctgcacc agaagttatt gaaacagcca
     4561 ttaattgctg tacatcgata ataccaaacg atgattactt ccacgttaaa gacactgatt
     4621 ttaatagtgt ttggcatgat atttacagag atattcgagc atctgacagc aatagtacaa
     4681 aaatatattt caacaatatt gaaataccac taaaattaat tgcagatcta attaatgagt
     4741 taggaattaa tgaattcatt gatagcaaaa aagagcttca aatgctaagt tacaaccaag
     4801 taaataaaat aatcaattca aacttccccc aacaagacct atgttttcaa acagaaaaac
     4861 ttttgtttac ttcactcttt caagaccctg catttatatc tgcactaaca tctgcattct
     4921 ggcaatctct tcacatcacg tcttcctctg tggaacacat atatgcccaa attatgtccg
     4981 aaaacataga aaacaggctt aattttatgt ctgaacaacg agttattaac aattgtggac
     5041 atattataaa aatcaacgca gttgtcccta aaaacgacac tgcaatctct gcctctgggg
     5101 ggagggctta cgaagttagc tcatcaatat taccatcgca cataacatgt aatggtgttg
     5161 gtattaataa aatagaaaca agctatctcg ttcatgctgg aacacttcca agcagtgaag
     5221 ggcttcgcaa tgctatccct ccagaaagtc gtcaagtatc atttgccatc atatctcctg
     5281 atgtttaagc caatattata ttggctgcca gactgtaaaa aaacgttctt cagaacagct
     5341 ctataaacta ttaaaccaaa gctaaaagga tggggaccaa tagaatctca gcccatcctt
     5401 tttatatcat tatgaaccgc cccggaaatc ctggagacta aactccctga gaaagaggta
     5461 aacaggatga ctaaaaatac acgtttttcc cctgaagtcc gtcaacgggc agttcgtatg
     5521 gttctggaaa gtcagggcga atataactca caatgggcag cgatttgttc cattgcc
//
LOCUS       DADMEQ010000180         5553 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.182, whole genome shotgun sequence.
ACCESSION   DADMEQ010000180 DADMEQ010000000
VERSION     DADMEQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.182"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..638
                     /locus_tag="JNZ05_003770"
     CDS             <1..638
                     /locus_tag="JNZ05_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566802.1"
                     /translation="GACIVLMELMMMVQLWWSVWLCWPVLSLLIVGRGCGGLGAITTG
                     KDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNAV
                     LDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALG
                     LSRSALVVAAWLLCYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(692..1297)
                     /gene="azoR"
                     /locus_tag="JNZ05_003771"
     CDS             complement(692..1297)
                     /gene="azoR"
                     /locus_tag="JNZ05_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAY8566803.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1498..5400
                     /gene="hrpA"
                     /locus_tag="JNZ05_003772"
     CDS             1498..5400
                     /gene="hrpA"
                     /locus_tag="JNZ05_003772"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HAY8566804.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRCIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANDGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1374 a         1358 c         1542 g         1279 t
ORIGIN      
        1 taggcgcgtg cattgtgttg atggagctaa tgatgatggt tcagttatgg tggtcagtct
       61 ggttatgttg gccagtatta tcgctactca ttgttggccg tgggtgcggt gggcttggcg
      121 cgataacaac agggaaagat agtcagggga aactcccgcc cgccgtttac tggctgacat
      181 tgccctggcg catcgggatg tggctatcta tgcgttggtt ttgtcgtcgc ctggagccgg
      241 tgagcaaaat gactgctggt gtttatttaa gggcgtttcc acgacatatt ccggcacaga
      301 atgcggttct ggacgtcacc tttgaattcc ctcgtggacg agcgacaaaa gatcgactct
      361 atttctgtgt accgatgttg gatctggtgg ttccggaaga gggggagctc cgacaggccg
      421 tggcgatgct ggaaacatta cgcgaagagc aaggcagcgt tctggtccat tgcgcattgg
      481 gattatcgcg cagtgcgctg gtagtggcgg catggctgtt atgttacgga cactgtaaaa
      541 cagttgatga agcgattagc tttattcgag ccagacgctc gcatattgtg cttaaggaag
      601 agcacaaagc gatgttgaaa ttatgggaaa acaggtaagt ggattgagat gtggactgaa
      661 tatctacagt ccacatcaag accgtgtccg gttatgcagc aacaatgctg tcgatggctg
      721 cttttgcgtc agactgtgct ttcgctgcca tttccggacc gtatgcgatc ccttcggcga
      781 agacaaattt cacatcggta atgccgataa agccgaggaa cgtggacaga tacggcgtca
      841 ccaggtccgt tggtccatct ttgtggatcc caccgcggct ggtaataacg atggcttttt
      901 tacccgttac cagaccttcc ggaccgttct cggtatagcg gaaagtaacg cctgcgcgtg
      961 caaccaggtc aaaatagttt ttcaactgag ttgagatgtt gaagttatac atcggtgccg
     1021 caataacgat aacgtcgtgg gctttcagct cggcaatcag ctcatcggaa agcgccagag
     1081 cttcctgctg acgcggagtc agcggcgcat cgctcggacg cagagcgcca accagttcgc
     1141 catccagtac cggaatcgga tttgcagcta ggtcgcgaac ggtgatttca tcagcggagt
     1201 gcttttcgcg ccattgttca acaaaataat cggacaactg attagactga gagtaccctg
     1261 ccaggatgct ggatttaaga actaatacct tgctcatggt gtttccttat agatgtttga
     1321 atggacgatg ccccgttgct tgctgacact ttattcacaa tcctgccaca gagatagcgc
     1381 aataaatcga agtctatgtt cgaatttatt gaacaacgca tagaaagccg cgatgtggta
     1441 ctctatatct atcatttaaa agaaaattaa tcaggcagac tgctgcccac taacgttatg
     1501 acagaacaac aaaaattgac ctttactgcc ttgcaacagc ggctggattc gctgatgctg
     1561 cgcgacagac tgcgtttttc tcgccgtctg cattgtgtta agaaggttaa aaatcctgat
     1621 gcacaacagg ccattttcca ggagatggcg aaagagattg accaggcggc agggaaagtc
     1681 ctgctgcgtg aagcggcacg accggaaatc acttatcctg acaatttacc ggttagtcag
     1741 aaaaaacagg acatactcga agcgattcgt gatcaccagg tggtgatcgt cgccggggaa
     1801 accggctccg gtaaaacgac tcagctaccg aaaatctgta tggagctggg gcgcggtatt
     1861 aaagggctga tcggtcatac ccagccgcgt cgcctggcgg cacgaacggt ggccaaccgt
     1921 attgcggaag agctgaaaac ggagccgggc ggttgcatcg gttacaaagt gcgtttcagc
     1981 gatcacgtaa gtgataacac gatggtcaag ctgatgaccg acgggatcct gctggcggag
     2041 atccagcaag accgcctgct gatgcagtac gacaccatca ttattgacga agcgcacgaa
     2101 cgcagcctga atatcgattt tctgctcggc tatttgaaag agttgctgcc gcggcgtcct
     2161 gacctaaaaa tcattatcac ttccgcgact atcgacccgg aacgcttttc gcgccacttt
     2221 aataatgcgc cgattattga agtctccggt cggacctatc cggtggaagt gcgctatcgc
     2281 ccgattgttg aagaagccga tgacaccgag cgcgatcagt tgcaggcgat ttttgacgcc
     2341 gtagacgaac taagccagga aagccccggc gacattctga tctttatgag cggcgagcgg
     2401 gaaattcgcg ataccgccga tgcgctgaac aagctgaact tacgccatac cgaaatcttg
     2461 ccgctttatg cgcggctttc gaacagcgaa cagaaccgcg tgttccagtc gcacagcgga
     2521 cggtgcattg tgctggcgac caacgtcgcg gaaacctcgc tgaccgtacc ggggattaaa
     2581 tacgtcatcg accccggtac agcgcgtatc agccgctaca gctatcgcac caaagtgcag
     2641 cgtttgccga ttgaacccat ttcgcaggcg tctgccaatc agcgtaaagg ccgctgtggt
     2701 cgtgtgtccg aagggatctg tattcgtctc tattccgaag acgatttcct ctcgcgcccg
     2761 gagtttaccg atccggagat tctgcgtacc aacctggcct cggttatttt gcagatgacc
     2821 gcgctggggc tgggcgatat cgctgcgttc ccgtttgtcg aagcaccgga taaacgcaat
     2881 atccaggatg gcgtgcgtct gctcgaagaa ctgggcgcga tcaccacgga tgaacaggcc
     2941 agcgcctata aactgacgcc gctcggtcgc cagctctcgc agttgcctgt cgacccacgt
     3001 ctggcgcgta tggtgctgga agcgcaaaaa catggctgcg tgcgtgaggc gatgattatc
     3061 acgtccgcgc tctccatcca ggatccgcgc gaacgtccga tggacaaaca gcaggcatcg
     3121 gacgaaaaac atcgtcgctt ccacgacaaa gaatccgact tcctcgcgtt tgtaaatctg
     3181 tggaattatc ttggcgagca gcaaaaggcg ctttcttcca acgccttccg tcgcctgtgt
     3241 cgtaccgatt acctcaacta tctgcgcgtg cgcgaatggc aggatatcta cacccagttg
     3301 cgccaggtgg tgaaagaact tggcattccg gtcaacagcg aaccggcgga gtatcgtgaa
     3361 attcacatcg ccttactgac cggtttgctt tcccatatcg gcatgaaaga tgccgataaa
     3421 caggaatata ccggtgcacg taacgcgcgt ttctccatct tccccggttc tggtttattc
     3481 aaaaagccgc cgaaatgggt aatggtggcg gaactggtag aaaccagccg cctgtggggg
     3541 cgcattgctg cgcgtatcga cccggaatgg gtagaacccg ttgctcagca tttgattaaa
     3601 cgcacctaca gcgaaccgca ctgggaacgg gcgcagggcg cggtgatggc aacggaaaaa
     3661 gtcactgttt atggtttgcc gattgttgct gcgcgcaagg tcaactacag ccagatcgat
     3721 tcggcgttat gtcgtgaact ctttattcgc cacgctctgg tggaaggtga ctggcagacg
     3781 cgtcacgcat tcttccgtga aaacctgaaa ctgcgggccg aagtggaaga gctggaacac
     3841 aaatcacgtc gccgcgatat tctggttgat gacgaaacgc tgtttgagtt ctacgaccag
     3901 cgcatcagcc acgatgtaat ctccgctcgt cacttcgata gctggtggaa aaaagtcagc
     3961 cgcgaaacgc ctgatttgct caactttgaa aagagcatgt tgatcaaaga aggggcggaa
     4021 aaaatcagca agctggatta cccgaacttc tggcatcagg gcaatctcaa gctgcgtttg
     4081 agctatcagt ttgagccagg cgcggacgcg gacggtgtga ccgtacatat tccgctgccg
     4141 ttacttaacc aggttgagga aagcgggttt gaatggcaga tccccggtct gcgccgcgaa
     4201 ttggtgattg ctctgattaa atcgttgccg aaaccggtac gccgtaattt tgtgcctgca
     4261 ccaaactacg ccgaagcgtt tttaggccgc gtcacaccgc tggagttacc gttgctcgac
     4321 agccttgagc gcgagttacg gcggatgacc ggcgttaccg ttgaccgcga agactggcac
     4381 tgggatcagg tgcccgatca cctgaaaatc accttccgcg tggtggatga caaaaacaag
     4441 aagctaaaag aagggcgctc gctacaagat ctgaaagatg cgctgaaagg caaagtgcag
     4501 gaaacgctgt ccgcggtggc ggatgacggt atcgagcaga gcggcttaca tatctggagt
     4561 tttggtcagc tgccggaaag ttacgaacag aagcgtggca actataaagt gaaggcgtgg
     4621 ccggcgctgg tggatgaacg cgacagcgtg gcgatcaaac tgtttgataa cccgctggag
     4681 caaaaacagg caatgtggaa tggtcttcgc cgtctactgc tgctaaatat ttcctcgccg
     4741 atcaagtatt tacatgagaa gttaccaaac aaagccaagc tggggctgta ctttaacccg
     4801 tatggcaaag tgctggagct gatcgacgac tgtatctcct gcggtgtgga tcaactgatc
     4861 gacgccaatg atggcccggt ctggacggaa gaaggctttg ctgcgctgca tgaaaaagtg
     4921 cgcgccgaac tgaatgacac ggtggtggat attgcgaagc aggtcgagca aatcctcact
     4981 gcggtgttca atatcaacaa acgtctgaaa gggcgggtgg atatgaccat ggcactgggg
     5041 ctttctgaca ttaaagcgca gatgggcggg ttggtatatc gcggttttgt cactggtaac
     5101 ggcttcaaac ggctgggcga cacgctgcga tatttgcagg cgattgaaaa acgactggaa
     5161 aaactggcgg ttgatccaca tcgcgatcgt gcacagatgc tgaaagtcga aaacgtccag
     5221 caggcgtggc agcaatggat caacaaactg ccgcctgcac gtcgtgagga tgaagacgtg
     5281 aaagagatcc gttggatgat agaagagctg cgcgttagtt acttcgctca acaacttggt
     5341 acgccttatc cgatttcaga taagcgtatt ttgcaggcga tggagcagat tagcggttaa
     5401 ccctgctatt tgcctaataa atctaaaacc cggtaagcat ttagcgccgg gttttttatt
     5461 aattctaaaa cggtaaggac aaaaattcag gaattcagaa aaatacaatt ctcagctgca
     5521 agatgaataa tgtttatcta cagcatttcc tta
//
LOCUS       DADMEQ010000181         5531 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.183, whole genome shotgun sequence.
ACCESSION   DADMEQ010000181 DADMEQ010000000
VERSION     DADMEQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.183"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            100..1008
                     /locus_tag="JNZ05_003773"
     CDS             100..1008
                     /locus_tag="JNZ05_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAY8566805.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1054..3045)
                     /gene="tkt"
                     /locus_tag="JNZ05_003774"
     CDS             complement(1054..3045)
                     /gene="tkt"
                     /locus_tag="JNZ05_003774"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY8566806.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3323..4081
                     /locus_tag="JNZ05_003775"
     CDS             3323..4081
                     /locus_tag="JNZ05_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY8566807.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4287..5207)
                     /gene="speB"
                     /locus_tag="JNZ05_003776"
     CDS             complement(4287..5207)
                     /gene="speB"
                     /locus_tag="JNZ05_003776"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HAY8566808.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IMGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5343..>5531)
                     /locus_tag="JNZ05_003777"
     CDS             complement(5343..>5531)
                     /locus_tag="JNZ05_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566809.1"
                     /translation="FQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNALPPCS
                     GQAFPADNNGYAPPPCRK"
BASE COUNT         1323 a         1483 c         1478 g         1247 t
ORIGIN      
        1 ttcgctatac ccctttatag attctgaatt ctcacctatc ggctgttttc tcctcatctc
       61 atgtgacata ttcccatact ttactcaagg aagattgtca tgaaagcacc tgtttcatta
      121 acctcatttc gcccacaaaa atctcttgct atcgccattg gcgtgcttgc cgtggttgtg
      181 ctgccatttc tctcgtatta caccgttaat gaaggtgaac gcggcatttt gctgcgctac
      241 ggaaaaatcg taaaagttgc cgagccgggg ctgggtttta agatcccttt tatggaaagc
      301 gtggagaaaa tttccacgcg taatcaggct gtggtctatc agggattaca ggcctacagc
      361 cgcgaccagc aaccggcaca aatgacagta tcagtcagct tccacattaa accttcagaa
      421 gcaggcgcgg tttataccac ctacaacact attgaagcat taaaagatcg tctaattgtc
      481 cgtcagttgc caactcagct ggagaacatt ttcggccaat ataccgccat cagcgccgta
      541 caggatcgta caaaattggt tcaggatctg caaaatgcca tgcgcaaagc cgttgttggg
      601 ccagtggtaa ttgacggggt ccagattgaa aatattgatt tttctgacgc ttatgaaaag
      661 tcgattgaaa accgaatgaa agcagaagtc gccattgcca cgcgtaagca aaatctggaa
      721 acagagaaaa tccaggcgca aatagcggta actcaggccc aggcagaagc agacagtaaa
      781 ctggcggcag ccaaagcaga agcggaaact attcgcgtaa gaggcgcggc agaggctgaa
      841 actattcgtt taaaaagtgc ggctgaagca gaagcaatcc gcctgcgcgg tgaagctctg
      901 cgggataatc ccggacttgt agccctcacc actgccgaac gttgggacgg taaactgccc
      961 gataccatga tccccggcag cacagttccg tttatctcta ctaagtaaaa aaagggccgc
     1021 tttcgcggcc cttctcacaa aacgtcttac tgattacagc agcgcttttg ctttcgcaac
     1081 aacgttatca acagtgaagc cgaactcttc aaacagcagc tctgccggag cagattcacc
     1141 gaaggtggtc atgccgacga tagcgccgtt caggccaaca tacttgtacc agtagtcagc
     1201 aatacccgct tctaccgcaa cgcgtgcagt aaccgctttc ggcagtacgg attcacggta
     1261 agccgcatcc tgcttgtcaa atgcgtcggt agacggcatg gacaccacgc gcgctttcac
     1321 gccttcggca gtcagttttt cgtaggcagc aaccgccagt tcaacttcag aaccggtggc
     1381 gatgaagatc agttccggct gaccagcaca gtctttcagc acataaccac cgcgcgcgat
     1441 gtttgccagc tgctcttcag tacgttcctg ctgcgccagg ttctgacggg agaggataag
     1501 cgcagtcggg ccgtcctgac gctcaacgcc gtatttccac gcgaccgcgg attcaacctg
     1561 gtcacacgga cgccatgtag acatgttcgg ggtcacgcgc agagaagcga cctgctctac
     1621 cggctggtga gtcgggccat cttcgcccag accgatggag tcgtgggtgt aaaccatcac
     1681 ctggcgctgt ttcatcagcg cagccatacg tacggcgtta cgtgcgtatt ccacgaacat
     1741 caggaaggtg gaggtgtacg gcaggaagcc accgtgcagg gagataccgt tagcaatcgc
     1801 ggtcataccg aactcgcgaa caccgtagtg gatgtagtta cctgcagcat cttcgttgat
     1861 tgctttagaa ccagaccaca gggtcaggtt agacggtgcc aggtcagcag agccgccgag
     1921 gaattcaggc aacagcgggc cgaacgcttc gatagcattc tgcgacgctt tacggctggc
     1981 gattttcgcc ggattagcct gcagtttagc gataaactct ttcgctttgg cgtcgaagtc
     2041 agacggcatt tcgcctttca tacggcgggt aaattcagcc gcttcctgcg gataagcttt
     2101 cgcgtaagcc gcaaacttct cattccatgc agattctttc gcctggcctg cttctttcgc
     2161 atcccactga gcatagattt cagacgggat ttcgaacggt gcatatttcc agcccagctg
     2221 ttcgcgggtc agggcaattt cagcgtcgcc cagcggcgca ccgtgggagt cgtgagtacc
     2281 ggctttgttc ggggaaccga aaccgatgat ggttttgcac atcagcaggg acggtttgtc
     2341 agtcactgcg cgcgcttctt caactgcgcg tttgatggat gccgcgtcat gaccgtcgat
     2401 gtcgcgaata acgtgccagc cgtaagcttc gaaacgcatt gctgtgtcgt cggtgaacca
     2461 gccttcaacg tgaccatcga tagaaatacc gttgtcatcg tagaacgcaa tcagtttacc
     2521 cagcttcagc gtacccgcca gagagcaaac ttcgtgggag atgccttcca tcatgcagcc
     2581 gtcgcccatg aaggcgtagg tgtagtggtc gacaatgtcg tggcccggac ggttaaactg
     2641 cgccgccagc gttttttctg caatcgccat accgactgcg ttggcaatac cctgacccag
     2701 cggaccggtg gtggtttcca caccagcggt ataacctact tccgggtggc ccggagtttt
     2761 agagtgcagc tgacggaagt ttttcagttc ttccatcggc agatcgtaac cggtgaggtg
     2821 cagcaggctg tagatcagca tggagccgtg gccgttggac agcacgaagc ggtcacggtc
     2881 agcccaggac ggattctgcg ggttgtgttt caggaaatca cgccacagga cttcggcaat
     2941 gtcagccata cccatagggg cacccgggtg accggatttg gctttctgta ctgcgtccat
     3001 gctcagcgca cgaatagcat tggcaagctc tttacgtgag gacattttga ctccagatcg
     3061 gatgatgaag ggcacgccct taacgacttg acgacagcgc gttttgggct acgccggaaa
     3121 atttgccaac aatttaccgc aagccgcgcg tcatgtacat ggaacatcct tttgccgctt
     3181 cagaaatctc tggatcatgc tcgcatgttg cgcaatctgc tcgcccgtcc gctgcgcttt
     3241 tccttatact gagactgagc gtcgattcac ctgcaaacgg cgcattttta gaataatcct
     3301 gaccttgtgc ggaagagaaa acatgaaaat tcgcgcctta ttggtagcaa tgagcgtggc
     3361 aacggtactg accggttgcc agaatatgga ctccaacgga ctgctctcat caggagcgga
     3421 agcttttcag gcttacagtt tgagtgatgc gcaggtgaaa accctgagcg atcaggcatg
     3481 tcaggagatg gacagcaagg cgacgattgc gccagccaat agcgaatacg ctaaacgtct
     3541 gacaactatt gccaatgcgc taggcaacaa tatcaacggt cagccggtga attacaaagt
     3601 gtatatggcg aaggatgtga acgcctttgc aatggctaac ggctgtatcc gcgtctatag
     3661 cgggctgatg gatatgatga cagataacga agtcgaagcg gtgatcggtc acgagatggg
     3721 gcacgtggcg ttaggccatg tgaagaaagg gatgcaggtg gcgcttggta caaatgccgt
     3781 gcgagtagct gcggcctctg cgggcgggat tgtcggcagt ttatctcaat cacaacttgg
     3841 tgatctgggc gagaaattag tcaattcgca attctcccag cgccaggaag cagaagccga
     3901 tgattattct tacgatcttc tgcaccaacg cggcatcagc ccggcaggtc ttgccaccag
     3961 ctttgaaaaa ctggcgaagc tggaagaagg ccgtcaaagc tcaatgtttg acgaccatcc
     4021 tgcatccgcc gaacgtgccc agcatattcg cgatcgcatc agcgcggatg ggattaagta
     4081 aagcctggtg gtgtcgaacg taggtcagat aaggcgttca cgccgcatct gacattaatg
     4141 gcacatttgt taccttgtgc gcattgccgg atgcgatgct ggcgcatctt atccggccta
     4201 cgggtgccga acgtaggtcg gataaggcgt tcacgccgca tccgacatta atggcacgtt
     4261 ttacccgtgc gcatcgcatc tggtgcttac tcgccctttt tcgccgcctg aatatacagc
     4321 atctccagcg ccagcgtcgc cgcagccaga gcggtgattt ccgactgatc gtatgccgga
     4381 gccacttcca ctacgtccat cccaacgatg ttgagatctt tcaggccgcg taccagttta
     4441 atggcgcgat cggaggtcag gccgccaatc actggcgtac cggttcccgg cgcgaaagca
     4501 ggatccaggc agtcgatatc aaaagtcagg taaaccggca tatcacccat aatctgtttc
     4561 acctgggcga taacgtcatc cacgctgcga tcgttcacct ggcaggcgtc cagcacggta
     4621 aagccgttgt ctttatcaaa ctcggtacga ataccaatct gcacggaatg attcgggtcg
     4681 atcagacctt ctttcggcgc ggtatagaac atagtgccgt ggtcaaattc acaaccgttc
     4741 gcataggtat cggtgtgggc gtcaaagtgt accagcgcca ttttgccgaa atgcttcgca
     4801 tgagcacgca gcagcggcag cgtaacaaag tggtcaccac cgaaggagag catacgctta
     4861 ccggcagcca gcagcttctc ggcgtgcgcc tgcagctttt cgctcatctc acgggcatcg
     4921 ccaaaggcat ataccagatc gccgcagtcc acgacgttca gacgctcacg catgtcgaaa
     4981 ttccacggga agcggttgtg ttcccaggcc agattcgtcg aaacctgacg gatcgctgcc
     5041 ggaccgtggc gaccacccgc acgaccagaa gtggccatat cgaacggcac gccagtaatc
     5101 acccagtctg catcgctgtc atacggctgg aagttcatcg gcaggcgtaa aaaaccaaag
     5161 gcattggaaa ccagtgagtt atcgtattga tgacctaagg tgctcatgga tggactccta
     5221 ttacaaagtc gatataaaaa aacccttccg cgtcgttagg cccgacgagg aagggttgga
     5281 tttgcgaaac acaagtttgt gctaattatc gccttaaatg ggcgaggcat caagcgaggt
     5341 tgctatttcc gacacggtgg cggcgcataa ccattgttat ctgccggaaa ggcttgtcca
     5401 gagcacggcg gcagcgcgtt ttgcgccggg ccttgtaatt caaaggcggc aaagccgatt
     5461 accccggtat tacgcacatc gccttgcgcc gaattggctg cgtaggaatc agcaacgttg
     5521 gcgaactgaa a
//
LOCUS       DADMEQ010000182         5405 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.184, whole genome shotgun sequence.
ACCESSION   DADMEQ010000182 DADMEQ010000000
VERSION     DADMEQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.184"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            244..1068
                     /gene="gadX"
                     /locus_tag="JNZ05_003778"
     CDS             244..1068
                     /gene="gadX"
                     /locus_tag="JNZ05_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HAY8566810.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1436..2164
                     /gene="gadW"
                     /locus_tag="JNZ05_003779"
     CDS             1436..2164
                     /gene="gadW"
                     /locus_tag="JNZ05_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HAY8566811.1"
                     /translation="MTYVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2527..>5405)
                     /locus_tag="JNZ05_003780"
     CDS             complement(2527..>5405)
                     /locus_tag="JNZ05_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HAY8566812.1"
                     /translation="STSDAAGNASITLTFETGTSPDIAQVQVQNKLQLAMPSLPEAVQ
                     QQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVASNIKDPLSRTAGVGSVQLFGSE
                     YAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQLGGMPQVADQQLNASIIVQTR
                     LQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYSTVARYNGKPAAGIAIKLAAGA
                     NALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIEISIQEVFKTLVEAIILVFLVM
                     YLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINTLTMFGMVLAIGLLVDDAIVVV
                     ENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLSAVFMPMAFMSGATGEIYRQFS
                     ITLISSMRLSVFVAMSLTPALCATILKAAPEGGHKPNALFARFNTLFEKSTQHYTDST
                     RSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEEDQGVFMTTAQLPSGATMVNTTK
                     VLQQVTDYYLTKEKDNVQSVFTVGSFGFSGQGQNNGLAFISLKPWSERVGEENSVTAI
                     IQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMELLDNGNLGHEKLTQARNELLSL
                     AAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVALSDINQTISTAFGISYVNDFL
                     NQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAPLSAYSSTEWTYGSPRLERYNG
                     IPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYSWTGLSYQEALSSNQAPALYAI
                     SLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLATDLRGLSNDVYFQVGLLTTIGL
                     SAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRPILMTSLAFILGVLPLVISHGA
                     GSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHLFARFKKA"
BASE COUNT         1418 a         1265 c         1279 g         1443 t
ORIGIN      
        1 tgtttattaa atgtagcgat tacctttaat gtcacgaaaa gggattattt gcttgctatt
       61 aatttcactg cgttcttaaa cacgtttttg tattataaat ccagtcatcc tgcccgtaga
      121 acatcacaca ttatcatcct gttctcccgc ttcgtttaaa tttatttatc aatcaatttg
      181 acttaagagg gcggcgtgct acattaatta acagtaatat gtttatgtaa tattaagtca
      241 actatgcaat cattacatgg gaattgtcta attgcgtacg caagacataa atatattctc
      301 accatggtta atggtgaata tcgctatttt aatggcggtg acctggtttt tgcggatgca
      361 agccaaattc gagtagataa gtgtgttgaa aattttgtat tagtatcaag ggatacgctt
      421 tcattatttc tgccgatgct caaggaggag gcattaaatc ttcatgcaca taaaaaaatt
      481 tcttcattac tcgttcatca ctgtagcaga gatattcctg tttttcagga agttgcgcaa
      541 ctatcgcaga ataagaatct tcgctatgca gaaatgctac gtaaaagagc attaatcttt
      601 gcgttgttat ctgtttttct tgaggatgag cactttatac cgctgctttt gaacgttttg
      661 caaccgaaca tgcgaacacg agtttgtacg gttatcaata ataatatcgc ccatgagtgg
      721 acactagccc gaatcgccag cgagctgttg atgagtccaa gcctgttaaa gaaaaaattg
      781 cgcgaagaag agacatcata ttcacagttg cttactgagt gtagaatgca acgtgctttg
      841 caacttattg ttatacatgg cttttcaatt aagcgagtcg cagtgtcctg tggatatcac
      901 agcgtgtcgt atttcattta cgtctttcga aattattatg ggatgacgcc cacagagtat
      961 caggagcgat cggcgcaggg attgccgaac cgtgactcgg cggcaagtat tgttgcgcaa
     1021 gggaattttt acggcactaa ccgttctgcg gaaggaataa gattatagag ttttactcag
     1081 acataaaaaa aacccggcat aggggaccgg gaagaggata gtctgccgtc tccagactaa
     1141 taaaccgtta taacactccc tgttggcacg ggaaactttg tgctctcagt aagttaaata
     1201 taacttttac tggaaataag atcagccatt tttttataaa cataagctat acgctgtgcg
     1261 aaaatatatt cgtgctgcat ttacttatta tcaattaact gttatgcaaa actactttgt
     1321 ggataaattt tggtcctacc aaatctggca gtttttgcgc taagaaacag tctgtcatca
     1381 tttcattagt atactgaaat tgaaataatc gcagtatgaa atataaggga taatcatgac
     1441 ttatgtctgc tcggtgatcc tcattcgtcg ttcattcgat atttatcatg aacagcaaaa
     1501 aatatcgctg cataacgaga gtatcctgct gctggagaaa aatttggcag acgattttgc
     1561 gttttgttca ccggatacgc gacgactgga tatcgatgag ctgacagttt gccattactt
     1621 acaaaatatt cgtcagctac cacgcaattt agggttacat agcaaagacc gtctgttaat
     1681 taaccagtca ccccccatgc cgctggtgac ggcgattttt gatagcttca atgaatccgg
     1741 ggtaaattca ccgatactga gcaatatgct ctacctttcc tgtttatcga tgttttctca
     1801 taagaaagaa ctgatcccct tacttttcaa tagcatcagc actgtttcag gaaaagttga
     1861 acgccttatt agctttgata tcgccaaacg ttggtatctg cgcgatatcg cggaaagaat
     1921 gtataccagc gagagtctca tcaaaaaaaa gttgcaggat gaaaatacct gtttcagtaa
     1981 aatattactc gcttccagga tgtcgatggc cagacgatta ctcgagttac gtcaaattcc
     2041 tctgcatact attgcggaaa aatgtggcta tagcagtacg tcgtacttta taaacacatt
     2101 tcgacaatat tatggtgtaa cgccacatca gtttgcgcaa cattcgccag gtaccttttc
     2161 ctgacatatt ttgcatttga atattggtca ggatctcaca cctgcttcat gtgaaactct
     2221 tccctgatga tttctgccgg gctaccggct agttctcttt cgcagtttgc caggctgtat
     2281 aggtattttc aggaggaaaa gactcggcat gtttgggatt attaagctga caattcatac
     2341 gattacggga atgtgggtca gtattgtgtt gttcaaattg atgacgaatg gctggagtgg
     2401 tttctacttt cagtgctgtg tactaagcct ggtattcctg acggttagct ggttgttgag
     2461 tggtgaatgg ttagcaggaa agagtaaggc tgaaccttca cgttcaacct tactctcatt
     2521 tacacgttac gcttttttaa agcgggcaaa gagatgttcc actacaacga aaaagaccgg
     2581 aacgaagtaa attgccagca ctgttgcggc aaacatcccg cccatcacgc cggtacctac
     2641 cgcgttttgc gcgccagaac cggcaccatg actgataacc agcggcagca cgccgagaat
     2701 aaaggccaga gaggtcatca ggattgggcg taaacgcatc cgcgccgctt cgatgattgc
     2761 ctctatcggc gttttccctt ctttctgcat catctcaacg gcaaattcga caatcaggat
     2821 ggcgttcttg gcggaaagtc cgatggtggt cagcaaacca acctggaagt agacgtcatt
     2881 acttaagccg cgcagatcgg tggccagtaa tgcgccaacg acgcctaacg gaacaaccaa
     2941 catcaccgag aacggaattg accaactctc atagagtgcg gcgagggcga ggaacaccac
     3001 gaccagtgaa atggcataca gtgcaggagc ctggtttgag gataacgctt cctgatacga
     3061 cagtccggtc catgagtagc cgacgcctgc cggaagttta gcgaccaggt ctgccataaa
     3121 tttcatagcg tcaccggtac ttttcccggc cgccgcttca cctaaaatct ccattgacgg
     3181 gatgccgttg tagcgttcca atcgcggtga accataggtc cattcggtag acgagtaggc
     3241 agaaagcggt gccatcgtgc cagaggcgtt gcgtacatac cattggttga tgttatccgg
     3301 caacatacgg aacggcgtgc ctgcctggac atacactttt ttcacccgcc cctggttgag
     3361 gaagtcgttc acgtagctga tgccgaaggc ggtggaaatt gtctggttga tatcagacag
     3421 cgccacaccc attgcctcag ctttcgcggc gttgacgttc actttgaaca tcggcgtatc
     3481 ttccaggccg ttcgggcgta ccccggtgac ctggtcaggc gattgcgctg ccagtgataa
     3541 cagctcgttt cgtgcctggg ttagtttttc gtgccccagg ttaccgttgt ccagcagttc
     3601 catatcaaaa cctgacgcgg tacccagttc agccaccgcg ggtaagttga acgggaagac
     3661 gacggcttta ttgatactgc ttaacgcaat cattgcccgc tgaatgatcg cggtaaccga
     3721 gttttcctca ccgacacgtt cagaccacgg cttgagactg ataaacgcca ggccgttgtt
     3781 ttgcccctgg ccgctgaagc caaagctgcc aacggtaaac accgactgaa cattatcttt
     3841 ctcttttgta agataataat ccgtcacctg ttgcagtact ttcgtggtgt taaccatggt
     3901 ggcaccggaa ggtaactgcg cggtggtcat aaataccccc tgatcctctt ctggtaagaa
     3961 agaggtcggt gtacgcagga acagcaccgc catcccggcg caaatcagca ggtagaccac
     4021 catgtagcga ccggtacaac gcaacagcga gcgggtgcta tcggtatagt gctgagttga
     4081 tttttcaaac agcgtgttga agcgtgcgaa cagggcgtta ggtttgtgac cgccttccgg
     4141 cgcggctttc agaatggtgg cgcacagggc aggggtcagg ctcattgcca caaatactga
     4201 aagccgcatg gaggagatca gcgtgatgga gaactggcgg tagatctccc cggttgcacc
     4261 gctcataaag gccatcggca taaacactgc agaaagaaca acggcaatcc cgaccagcgc
     4321 acgttggatc tgccccatcg atttatgcgt cgcttccttc ggcggtagct tatcttccgc
     4381 aatgacacgc tcgacgttct ccaccaccac gatggcgtca tccaccagta accctatcgc
     4441 cagcaccatc ccgaacatag tcaacgtgtt gatggtgaaa ccgaccgccg acaagatcgc
     4501 aaacgtcccg agaataacca ccggtacggc aattgtcggg atgatggtgg cacggaaatt
     4561 ctgcaaaaac agatacatga ccaggaagac gaggatgata gcctcaacca gtgttttgaa
     4621 aacttcctga atagaaattt cgataaacgg cgtggtgtcg taaggataaa ccgtcttcag
     4681 acttgcgggg aaataggctg ataagcggtt cagctcctct ttgactgccc gcgaggtatc
     4741 cagggcgttt gctccggcag ccagtttgat ggcgatcccg gcagcaggtt tgccgttata
     4801 gcgcgccacg gtggaataat cttccgcccc aagttcgacg cgggcgacat cacgcagcag
     4861 cacttgcgaa ccatcttgct gaactttcaa caggattttg ccaaattctt ccggcgtttg
     4921 cagacgcgtc tgcacaatga tcgaggcgtt tagttgctgg tctgccacct gtggcatgcc
     4981 acccagttga ccaccagaaa tctggttgtt ttgcacctta atctgggaaa taacatcgga
     5041 aggtaccagg ttatatttat tgagtttttg cgggtccagc cagatacgca tagcgtactc
     5101 ggaaccaaag agctgtacgc taccaacgcc cgcggtacgg cttagcgggt cttttatatt
     5161 agacgctaca tagtccgcga tatcgtactg gttgaggctg ccgttatcag aaataaacgc
     5221 cgctaccatc aggatattac tgctcgactt atcgacgcta atcccctgtt gctgcaccgc
     5281 ttcaggtaat gaaggcatag cgagttgcag tttattttgc acctgaacct gtgcgatatc
     5341 aggagatgtc ccagtctcga aggtcagagt gatagaggca ttacccgccg catcactggt
     5401 tgaag
//
LOCUS       DADMEQ010000183         5392 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.185, whole genome shotgun sequence.
ACCESSION   DADMEQ010000183 DADMEQ010000000
VERSION     DADMEQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.185"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..446)
                     /locus_tag="JNZ05_003781"
     CDS             complement(<1..446)
                     /locus_tag="JNZ05_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HAY8566813.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(501..1352)
                     /locus_tag="JNZ05_003782"
     CDS             complement(501..1352)
                     /locus_tag="JNZ05_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAY8566814.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALSAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1365..2165)
                     /locus_tag="JNZ05_003783"
     CDS             complement(1365..2165)
                     /locus_tag="JNZ05_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY8566815.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2228..3199)
                     /gene="manX"
                     /locus_tag="JNZ05_003784"
     CDS             complement(2228..3199)
                     /gene="manX"
                     /locus_tag="JNZ05_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAY8566816.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAANRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3662..5212
                     /gene="yoaE"
                     /locus_tag="JNZ05_003785"
     CDS             3662..5212
                     /gene="yoaE"
                     /locus_tag="JNZ05_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HAY8566817.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFFFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5216..5389)
                     /locus_tag="JNZ05_003786"
     CDS             complement(5216..5389)
                     /locus_tag="JNZ05_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rtn protein"
                     /protein_id="HAY8566818.1"
                     /translation="METQEQAKYLRRHGVHILQGYLYAQPMPLRDFPKWLAGSQPPPA
                     RHNGHITPIMSLR"
BASE COUNT         1311 a         1361 c         1373 g         1347 t
ORIGIN      
        1 acgagaagct tataaatttt ttccagatcg tcgatatttc gaacgcggat taacagccga
       61 cgctgttcta attgcatcac caacacgcca tcttccgaca agttcatcgc tttgattcgg
      121 ctatattcaa tccagacatt ggcgaagaaa aaaccttttt gtttaaagat gatcttcgga
      181 atgcggatcc agaatatata aaaacccatc agagccagtg cacttaataa ccatgttgtt
      241 attagcgcgc catgattcgt gacgttgtta tagataagaa tgacaatcaa tccgacgaag
      301 ataacgctat cgatgcgacc acgccggagc aaaggaattg ccagcagggt ggggccgtta
      361 cggcggggca tgatgaactg atcgtagatc gcgaaggcca ggagtgcggc gatgaaaaga
      421 atcagtacca ggtccgtgat tgtcattaat cctccagata aaaaaacggg gccaaaaggc
      481 cccggtagtg tacaacagtc ttacagtccc agcaggccgc aagcgtaacc agcgataccg
      541 atgacgaaga agccaacgat gatccacagc gggttaactt ttttgcgcag cagccacata
      601 caagcaaatg tcagcagcag tggtacaagg cctggcatta actggtcaag aatagtttgc
      661 acggtggtga cacgggtctg tccatcctgc ccggtgatgg tcgaaaccac cagcgggatg
      721 ttgacatgtg tccacttgtt aaccaatgcc cccatgacaa acaggccgag gatagacgcc
      781 ccttccgtca gtttttgcag gaagccacca cccatatctt taacgatatc gatacctttg
      841 gagtaaccat acgctacgcc gtagtaacgg gttgccagac gcaccaggtt aaacaggatg
      901 aagaacagca gcggacctaa caggctgccg ctcatcgcga taccggcact cagtgctgca
      961 aataccggac gtacggttcc ccagaagatc gggtcgccta caccagccag cggccccatc
     1021 aaaccgactt tgataccgtt gatagcaccg tcatcgatct ctgcgccatt agcacgctgt
     1081 tcttccagcg ccagggttac gccgagaatc ggtgcagcca cgaacggctg ggtgttaaag
     1141 aactccaggt gacggcgaat agcttgttta cgagcttcgt tgttctcagg gtagaggcga
     1201 cgaattgccg gtaccataga gaagcagaaa cccagtgcct gcatacgttc gaagttccat
     1261 gaaccctgga agaggttaga acgcaggaat acgccacgaa tatcactttg agtgagtttt
     1321 ttctcggtgg tagtttgagt tgtatcaacc atttcgctca cctgttagtc cagttcgtta
     1381 tcgagatcgt tgttaccagc tgcctgagca ggcgcaccgg ctacgcggtt gtatttcggg
     1441 ctaagttgga tgtagagcac tgccataaca gtaccaatca cacccagagc aaccaggtta
     1501 aagttggtga atgctgcggt tacgaagccg aggtagaaga acggcatcag gtagccagca
     1561 cgcatcatgt tgataaccat cgcataacca accaccacga tcatgccccc ggcgatattc
     1621 agaccattgg tcaccacttc cggaatcgca ttcagcatgt tctgtacttc gctggtacca
     1681 acagacagcg caacgatgac ggccggaata gccacacgca ttgcttgcag gaacagagaa
     1741 gaaacgtgga tccaggaaat cgctgtcagg ttgccgttat cagcagcctt atccgcagcg
     1801 tgctggaaag caacggtaat agtacgaacg atgatggtca gtacctggcc cgcagcggcc
     1861 agagggattg ccagtgcgat acctgcacca atgctctgat gacctgcgat aaccagaatg
     1921 gtagaaatga tagaagccag agcggcgtca ggcgcaactg cagcaccgat gttcatccag
     1981 cccagcgcga tcatttccag cgtaccaccg ataataatac cggttttcat atccccaaga
     2041 acgataccca ccagggtaca cgcgattagc ggacggtgaa actgaaattc atcgaggatt
     2101 gatcccatac ctgcgataca ggctacgata aataccagca caatttgaag agtggtaatc
     2161 tccattgtac ttctcctgtt tacgtaagac ttaagtgtga aaactgaatg ataatcaaca
     2221 caatacgtta cttatcgatt ttgctgatca gatccatcat tttcagtttc ggatcggtgg
     2281 aaaccttacg gacttccagc tcaataccgc gtgcattcag ttttttgaac gcctcgatat
     2341 ctttttcatc aaccgaaacc gcgttattca cctgggtttt accctgacgg aatgccatac
     2401 caccgacgtt aacagaggtg attttcacgc cgccttcaac gagacgctct acatctgttg
     2461 ggttggtaaa taacagcatt acgcgttcgc cagcatattt cgggttgttg tagacgcgaa
     2521 tcattttggc aacatcaact acgtgtgctg ttacgcccgg aggtgcaacc tgggtgagca
     2581 gtgtcttacg aacggtatcc gcagccactt catcactaac aacaataata cgggagacat
     2641 tggtttcttt ggtccagcgg gtggcgacct gaccgtgaat cagacggtcg tcgatacgcg
     2701 caaggccaat aaccatgtag tcgtttggcc ccattggttt tgccggagtt ggagccgctt
     2761 ttggtgctgc ggcagcgggt gctggcgcgg ctttttcaac cggtttggct ttcagtgctt
     2821 tcacgccttc acggcctgtt tctactgcca gcgcaaccag ttcatcaaag ctcgggtcat
     2881 catcacgggc cattaacgtt tccacgagca ttggaatgtt aacgcctgca atgacttcat
     2941 aatgctcttt gtcgacgaca atgcggttgg cagcattgaa cgggctgcct ccccatgtat
     3001 caacgagaaa cagcacgcct ttagtggtgt cgagttttgc caactgagcg ttgtattttt
     3061 caatcagcgt ttcggcattt tcacctggaa cgaaatcgat ccagccgacg ttttcctgct
     3121 cgcctaacag catttctgcc gttttaagca actgctctgc agcccaacca tgtgtgccta
     3181 taacaatagc aatggtcact tgctacctcc tttattatcg ttaacacctc aacgtgccag
     3241 atgtattttt gaatcgtcgt ccacaatcga atcgattcag ataagggcta caaccaaaaa
     3301 cccctgcgtt tcgcgattta ttttagatat cgaaaaaatt attttatgtg atgaagatcc
     3361 gtaatttaac tttcgattat cagaaatttc gaaaggtaaa atatccttgt cacattcgtt
     3421 tgcaaaggaa ggtaaatctt tgccaaatca gaggcgtctc tgatatgttt aactcccgtt
     3481 taacaaccat ggagtatagg gcagtagccc gcagtatgga tcgtcaccga cgtcatttca
     3541 gcatcaggcc ttttaacgcc tgcctttctg gcactctatg ccgcaccttt cgtttgcatt
     3601 ttgtcgttac gcctgcatta tttctggcgt cgaatagcta ttccttaagc aggagcttgt
     3661 catggaattc ttaatggacc cctcaatttg ggcggggcta ctcacgcttg ttgttctcga
     3721 aattgtgctg ggtatcgata acctggtctt catcgccatt cttgctgaca aactgccgcc
     3781 aaaacaacgc gataaagcgc gtttgctggg gttatcactg gcgctgatta tgcgtctggg
     3841 gctgctgtcg ctgatttcat ggatggtcac gctgaccaaa ccgctattta ccgtcatgga
     3901 tttcttcttc tccggacgcg atctgattat gttgttcggg gggatattct tgctgttcaa
     3961 agcaacaacc gaactgcatg aacggctgga aaaccgcgat catgattccg gccacggtaa
     4021 aggctacgcc agtttctggg tggtcgtcac acagattgtc atccttgacg ccgtcttctc
     4081 gttggatgcg gtaattactg cagtagggat ggttaaccat ctgccggtga tgatggcggc
     4141 ggtagtgatt gcgatggcgg ttatgttgct ggcatcgaaa ccgctgacgc gattcgttaa
     4201 ccagcacccc acggtggtgg tgctctgtct gagcttcctg ttaatgattg gtctgagtct
     4261 ggtggcagaa ggtttcggtt tccacattcc gaaaggttac ctgtatgccg cgattggctt
     4321 ctcgatcatt atcgaagtgt ttaaccagat tgcgcgtcgc aactttattc gtcaccagtc
     4381 gactttgccg ctgcgagcgc gtactgccga tgccatcctg cgtttgatgg gcgggaaacg
     4441 tcaggccaat gttcagcacg atgccgataa cccgatgccg atcccggaag gtgcatttgc
     4501 cgaagaagaa cggtacatga ttaacggcgt actgacgctg gcgtcgcgtt ctctgcgcgg
     4561 gatcatgacg ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat
     4621 ccgcgagcaa ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga
     4681 tgaaatcatc ggtatcgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga
     4741 tgtggcggcg attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat
     4801 caacctgctg ggcgtgctgc gtcgcgctcg cgggagcttt gttatcgtga ccaacgagtt
     4861 tggtgtggta caaggcttgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt
     4921 cccggacgct gacgaaacgc cggaaatcat taccgatggt aacggctggc tggtaaaagg
     4981 cggtacagat ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga
     5041 tgatatcgcg acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg
     5101 cgatgtgatt gatgtagggc cactgcatat caccatcctt gaagccaatg attatcgtgt
     5161 tgatctggtt cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcgttaac
     5221 gtaacgacat aatgggcgtg atatgtccat tatgccgggc gggcggcggt tggctgcccg
     5281 ccagccattt gggaaaatca cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa
     5341 tatgcacgcc atgacggcgc aaatacttcg cctgttcttg cgtttccacg cc
//
LOCUS       DADMEQ010000184         5358 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.186, whole genome shotgun sequence.
ACCESSION   DADMEQ010000184 DADMEQ010000000
VERSION     DADMEQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.186"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(694..1743)
                     /locus_tag="JNZ05_003787"
     CDS             complement(694..1743)
                     /locus_tag="JNZ05_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY8566819.1"
                     /translation="MYNNKIDNNRKTRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSIFDYIKSKFYRLMIPYFTFAFTILIINTINSGETI
                     DYIYSHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFKEIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            complement(2464..3402)
                     /locus_tag="JNZ05_003788"
     CDS             complement(2464..3402)
                     /locus_tag="JNZ05_003788"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566820.1"
                     /translation="MSLLLIVMMPSDVFFNAAIWITGSFNYLWPSAIAFIGYSLLIKK
                     INMENHEKTQSLICYLLFVIFYFLFFLSSFNEQIAVVNVLLCTTLLIFCKTNNYNTKP
                     LLNAMGISLLVIIYIATCPGNKVRYYAEIEHWFKEYGNFNIIQRSMLGLNLYADMLFS
                     VKSIIPALLAFSSTIICKKKTKILPLISCCILITLYIIHTPPVIFQAIHFSESNLFST
                     LSVFRVSFAMILTALIIFPTVISLNFNVTSIFISTMIIGTIATTSMLGLSPSIYASGN
                     RIYFIPYLLLITAIVVSTPIAINNIVANFAKSHYKI"
     gene            complement(3723..4640)
                     /locus_tag="JNZ05_003789"
     CDS             complement(3723..4640)
                     /locus_tag="JNZ05_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY8566821.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIIFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKDKK"
     gene            complement(4637..4999)
                     /locus_tag="JNZ05_003790"
     CDS             complement(4637..4999)
                     /locus_tag="JNZ05_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAY8566822.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKASTTAMRYMLYVGFMGILSAIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
BASE COUNT         1750 a          969 c          975 g         1664 t
ORIGIN      
        1 tacaatgatt tcaacttatt ttatttaatt gatattacga aacttaactc atacataaac
       61 aatatgactc aatactgtcc taaatagctg cgcagaaaag tgctccactt tcgctcagcc
      121 cttgctgggc gaagcatcag gaaggcgttc tcagctaggt tgtgttatat ctggcttgag
      181 ttttgttgcc catacgcaac acattaccca tctgtaaacg acaagtgtga cagaattagg
      241 tgcagctatt tagactatat cgacgcagta acgataatag atatcgaaac tgtactagat
      301 attagctact tatggttacc ggggggggtc ttttgagcac tggtgttatc aatcggcttc
      361 aaataattca ccaataaatt atattgcgat agctcatcac cttccaggtt gtttatagcc
      421 agtttatcag cgtcaccacc tactgtttat gcttttattt ctttgattaa taagtatatg
      481 atattttaat tcatctggtg tatttctttc tcatgtgttg gccgactgaa tatctgttca
      541 gtgaaggggt aattttatgt ttgcacttcg aacattccct tcagttgcta ggaaagaaaa
      601 ccgtaacatt tgcgcattaa ttttaacagt ataatctgag cataaatatg cgctatatat
      661 ctataaaaag gcgcaattaa ttgcgccttt ttatcatttt atcaaaaatg gcccaccgac
      721 tgtcctcgat agccaatggc caattttatc tgatacatat acacaaatac aggtatatat
      781 gattgcgcag ataggatttg gaatggtcac gctgaagcct atgattgcta aagccatagg
      841 gataaataat ttggtgatat actccattcc acatatgtta agagtatttc ttccagctct
      901 tacaataaag tcattcccac aaattatttt tgctataaaa atattaaata ttatcaaacc
      961 taccgtggtg ataatagtat taatagcttg aatataacta cttgatatta tgtctgcatt
     1021 gaatattgag ataatcttaa aaagcaaagg tttctggtta aggagttgat acgcactcat
     1081 tatcgcggta attgagaaga caatgaaacc aattttagat tttccaaaaa acctgtcacg
     1141 tgttaattct aaaaacatac atctaccgag aggcaataac caccagtatg ccatagcgga
     1201 gtctatgttc caaaaccatt gaggatctag taatgggtta tgattgagca ctgtttgtga
     1261 caacatgaac gataatagtg atatgattaa aatcactata ttgttcttga ctatttcttt
     1321 aaagattgca tcaattgcta ttataacaaa aaggcagttt ataaaccata ttgttcctac
     1381 aaattggttg tttctaacac catatattat atcataaatg tggctatata tataatcaat
     1441 cgtctcacct gagttaattg tgtttattat taatatagta aatgcaaaag tgaaatatgg
     1501 aatcattaat ctgtaaaact ttgacttgat ataatcgaaa atagataagt catttttttt
     1561 gatagtaaaa aaaccagcag cgaaaaagaa tagtgggacg tgataactaa aaacaaaagg
     1621 atacaatttg ccagccccca aaccaaggtg acctaaatat atagcaaaaa taccgagaaa
     1681 ttttaaagca tcaacccaat catgccttgt ttttctgttg ttgtcgattt tgttattata
     1741 catttaaagt aggctctcca gttttgtgtg attttcaaac actcattgca aatatgaatg
     1801 cctgaaagtt tatgattatt aatcaacgag ttcaatatta atttattatt tttcaaagtc
     1861 gcactatata ggcatttact tccgcagtac caccgatagt atcgatatca aatcagcctg
     1921 cggccgcgat aactttcgta acatcctgcc agcggctttg ttgaataaat caggttcaag
     1981 acaatcgtct ctgcatcagg ttagagtctc aggcaataca cactctttct ggcatagctg
     2041 ccactctcgc accatggcca tagcctttac gatctgcata ctcaccagac cagagcccgc
     2101 tccctttcgg ggaggaatga gggctctgat attcttttgc cgtagttcat cgtgaaaccg
     2161 tcggatgtca tttggcctga actaccagtg gtgtaggcca ttcaatatcg tattatattg
     2221 aaatataaat atgcatcagc tgcacacggt atatttccat gccagaagaa gtatcctgac
     2281 gtgaaacatg actttgttgt caggatttca tgagagcggt tcagcaaaaa atgacgaaaa
     2341 tcctagtata gtcgggtgat gagcagtata gaggaatgtg ttataaccaa taaaagtcca
     2401 attaaatttg tttttatttc taagcccccc ccccaaaaaa aggaggtggc ttttaaattt
     2461 aattcaaatt ttataatggc tcttggcaaa atttgctact atgttattta ttgcgattgg
     2521 ggttgaaaca acaattgctg ttataagaag caaatatgga ataaaataaa ttctattacc
     2581 agaagcatat atgcttggtg ataatcctaa catagatgtt gtggcaatgg ttccaataat
     2641 cattgtggat atgaaaattg aagtgacatt aaaattaagt gatattacgg ttggaaatat
     2701 aatgagtgct gtaagtatca tggcaaaaga caccctaaac acggagagtg tcgagaaaag
     2761 attgctttca gaaaaatgta tggcttgaaa aataactggc ggtgtatgta taatatatag
     2821 tgtgatcaag atacagcaag aaattagagg gagtattttt gtttttttct tgcatataat
     2881 agtactggaa aaagctagta atgctgggat aattgatttt acagaaaata acatatctgc
     2941 atataagtta agtccaagca tagatctttg gattatatta aaattaccat actctttaaa
     3001 ccaatgttct atctctgcat aatacctgac tttatttcct gggcaagttg caatataaat
     3061 aataactaat agtgaaatcc ccatcgcatt taataatggt tttgtattgt aattgtttgt
     3121 tttgcaaaaa attaacagtg tggtgcataa taatacattg actacagcta tctgttcatt
     3181 gaatgatgat aaaaaaaata aaaaataaaa aataacaaat aacaaatagc aaattagaga
     3241 ttgagttttc tcgtggtttt ccatattgat tttttttata agtagggaat aaccaataaa
     3301 tgcaatagca cttggccaca gatagttgaa tgatcctgtt atccaaatcg cggcattgaa
     3361 aaaaacatcg cttggcatca taacaatcag taataatgac atgattgaag tgtttatttt
     3421 atagtaaagg ttgttttttc tctgaaagat agagcaaatg ccaataatta atgatgaaaa
     3481 cattgctgca ttaattaaag accaagcata aaaatgatta ataagattta ttaatgcata
     3541 ttcaatctat gttcgtgatg accatgtgaa atatctatct ataagccact tgatgtgatc
     3601 cggttctgat attgatttct cggaaaacca aatgtcatct gatactagtg tatattgatt
     3661 taaatgaaga atagttaaac caataaaaaa aagcaaaaga aagcatatgt aatttgattt
     3721 aatcatttct tatccttaat aatatattta ggtctatttt ttacttcaat atatattctg
     3781 cctatatatt ctccgagaac accaatcccg atcagttgca cgccacccaa gaaaagtatt
     3841 gatacaagca gggagggata cccgcgtact gggttaccaa agacaagggt gtctataatc
     3901 atccatgcac catataaaaa cgaaatgctt gcaacaaaca agcctatata agtccatacg
     3961 cgaagaggaa aggttgaaaa acttgtgata ccttccagtg ccagattcca taatttccag
     4021 ccattaaatt ttgagatgcc agcaacacgc tctgcgcgta catattcaac gacatctgtc
     4081 tgaccaccca cccagctcag tatgcctttc atgaaaagat tgcgctcagg caacagttta
     4141 atgttctcca caacctcacg agacatgagt cgaaaatctc cgacattttc ctcgatcttt
     4201 ggggtgctta ttttgttgtg taacttatag aaccactcag ctgtcttacg tttcagtcgt
     4261 ccatcagttg agcggtctga acgtttcgca agcaccatgt cagcaccagc ctgccacttt
     4321 tcaataagat gagggataac ctcaataggg tcttgcaggt cgacatcaat aggaattaca
     4381 gcatcgccgc ttgcatggtc taaccctgca aataaggctg gttctttacc aaagttgcgt
     4441 gtaaatgaca gcggaacaac tagcgggtct gaaacagcca gcgcgttaat aattgactct
     4501 gtggcatctt tacttccgtc atttatgaat ataatttcta cttcatatgg cttcaactct
     4561 tggaattcac gtaccgtctt atagaaaaca ggtatcgctt cttcttcatt gaagacagga
     4621 acgacaagag atattttcat ttcgcatccc taaagacaat gaactttgaa tagacgaaac
     4681 cgcacaccag gctgatggcg gagaaggtga taagagtgac tattggagga agtgagcatt
     4741 tatcagcagc ccaaccaaca atcgcactta atattcccat gaatcccacg tataacatgt
     4801 agcgcatcgc tgtagtcgat gctttgaatg tgaatcttgc attcgcgaag aagctaaagc
     4861 tcacagccac tacgaaacct gtgaagtttg ccagggcctg actggtatgt gcggcataga
     4921 tacatacacc aaaaaccacc cagtgtataa gtgtgttcag cacacctata gatgtgtact
     4981 ttacaaataa ctttaacatt tatttaatca atgagctctg aaaggcatga agtctatcat
     5041 ccaagtctca attgatcgat acttgctatg tctgatgaga caaaactgag acacataagg
     5101 cctcacaatg gcttgcaagg ctttacatgt tttgatgtgg tgggacgtga gagcgcagtg
     5161 ttgatgggat aatctcttga attacaagcg gattcttata attcgtaatg cgaaggtcgt
     5221 aggttcgact cctattatcg gcaccattta aatcaataag ttacacatca ttagtacctt
     5281 ccttattttt tgactgggac aaatttggga ccgatgggtt caggatcgag tctatttgcc
     5341 gtgcgtgttc ggtaaggt
//
LOCUS       DADMEQ010000185         5337 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.187, whole genome shotgun sequence.
ACCESSION   DADMEQ010000185 DADMEQ010000000
VERSION     DADMEQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.187"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="JNZ05_003791"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="JNZ05_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HAY8566823.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="JNZ05_003792"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="JNZ05_003792"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HAY8566824.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINSEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNSLLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="JNZ05_003793"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="JNZ05_003793"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HAY8566825.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="JNZ05_003794"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="JNZ05_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAY8566826.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEINEDRIVAV
                     Q"
BASE COUNT         1309 a         1289 c         1455 g         1284 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaactc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaattcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggt
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaaattaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggg
//
LOCUS       DADMEQ010000186         5314 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.188, whole genome shotgun sequence.
ACCESSION   DADMEQ010000186 DADMEQ010000000
VERSION     DADMEQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.188"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            9..746
                     /locus_tag="JNZ05_003795"
     CDS             9..746
                     /locus_tag="JNZ05_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY8566827.1"
                     /translation="MSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNV
                     TYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATLG
                     SGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSF
                     YLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLGL
                     SATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="JNZ05_003796"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="JNZ05_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HAY8566828.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3607..3804
                     /gene="safA"
                     /locus_tag="JNZ05_003797"
     CDS             3607..3804
                     /gene="safA"
                     /locus_tag="JNZ05_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HAY8566829.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
     gene            3878..4639
                     /gene="ydeO"
                     /locus_tag="JNZ05_003798"
     CDS             3878..4639
                     /gene="ydeO"
                     /locus_tag="JNZ05_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HAY8566830.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTEPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5041..>5314
                     /locus_tag="JNZ05_003799"
     CDS             5041..>5314
                     /locus_tag="JNZ05_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566831.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVV"
BASE COUNT         1531 a         1131 c         1178 g         1474 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgaa aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ctactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagcacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ctgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg cattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tactcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaaca ttatccgcta aataaacata
     3601 tttgaaatgc atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa
     3661 atcatgtctg caattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg
     3721 gcaattgatc agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc
     3781 attatcaata gtttgcaaga ctgaaaataa acagcaaatt ataaatgtga aatgtttaaa
     3841 aggtatcgat aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat
     3901 atttattcat catgctttca acgctaacat tttagataaa gattacgcct tctctgacgg
     3961 cgagatcctg atggtagata acgctgttcg tacacatttt gaaccttatg agcggcattt
     4021 taaagaaatc ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca
     4081 gacagtgacg gagcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt
     4141 gcttaatgaa atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga
     4201 acttttgctt ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt
     4261 aactaacggt gtgctatccg tttccgggaa agtgagaaat attgtcaaca tgaagctggc
     4321 gcacccatgg aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa
     4381 gaaaaaacta aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca
     4441 gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg
     4501 ttatgccagt acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa
     4561 gagagtttct aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat
     4621 gagtgcttta gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga
     4681 attatatggg gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat
     4741 aaataagtga catccatcac atatttatgc acttgcataa cctgttgcat gattatttat
     4801 aatctcaatt cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag
     4861 ttgtttatcg gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact
     4921 gttatttccc tttttttagc taaatctgct atctctttat gtgactaact tcacttacat
     4981 ccacttattt ctcttcgtaa aattacttcg gaattaagta caataagaag aggaacattt
     5041 atgaagtctg cattaaagaa aagtgtcgta agtacctcga tatctttgat actggcatct
     5101 ggtatggctg catttgctgc tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca
     5161 aacgttgctt tctcagactt tacgccgact gaatacagta ccaaaggaaa gccaaatatt
     5221 atcgtactaa ccatggatga tcttggttat ggacaacttc cttttgataa gggatctttt
     5281 gacccaaaaa caatggaaaa tcgtgaagtt gtcg
//
LOCUS       DADMEQ010000187         5294 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.189, whole genome shotgun sequence.
ACCESSION   DADMEQ010000187 DADMEQ010000000
VERSION     DADMEQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.189"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            1..583
                     /locus_tag="JNZ05_003800"
                     /pseudo
     CDS             1..583
                     /locus_tag="JNZ05_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            647..1573
                     /gene="glsB"
                     /locus_tag="JNZ05_003801"
     CDS             647..1573
                     /gene="glsB"
                     /locus_tag="JNZ05_003801"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HAY8566832.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEVAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            1573..1932
                     /locus_tag="JNZ05_003802"
     CDS             1573..1932
                     /locus_tag="JNZ05_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HAY8566833.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQPPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            2071..3489
                     /locus_tag="JNZ05_003803"
     CDS             2071..3489
                     /locus_tag="JNZ05_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY8566834.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIELIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            3716..5167
                     /locus_tag="JNZ05_003804"
     CDS             3716..5167
                     /locus_tag="JNZ05_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAY8566835.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
BASE COUNT         1225 a         1233 c         1423 g         1413 t
ORIGIN      
        1 cccatcattc ttgcaggtaa cggctactta cttaaacatg cgccgaatgt gatgggctgt
       61 gctcagctca ttgcccaggt gtttaaagat gcgggaatcc cgcaaggcgt atatggctgg
      121 ctgaatgccg acaacgacgg tgtcagtcag atgattaaag actcgcgcat tgctgctgtc
      181 acggtgaccg gaagtgttcg tgctggagcg gcactgaaaa aatgcgtact actatccgcc
      241 aacggttctg gcgaatgtta ccccagaaat gaccgcgttt cgggaagaaa tgtttggccc
      301 cgttgctgca atcaccattg cgaaagatgc agaacatgcg ctggaactgg ctaatgatag
      361 tgagttcggc ctttcagcga ccatttttac caccgacgaa acacaggcca gacagatggc
      421 ggcacgtctg gaatgcggtg gggtgtttat caatggttat tgtgccagcg acgcgcgagt
      481 ggcctttggt ggcgtgaaaa agagtggctt tggtcgtgag ctttcccatt tcggcttaca
      541 cgaattctgt aatatccaga cagtgtggaa agaccggatc tgacgcgaca tattcagctc
      601 tgatatactc gcaggtcttt tcagacctgc ggtccaggag tagaaagtgg cagtcgccat
      661 ggataatgca attttagaaa acatcttgcg gcaagtgcgg ccgctcattg gtcagggtaa
      721 agtcgcggat tatattccgg cgctggctac agtagacggt tcccgattgg ggattgctat
      781 ctgtaccgtt gacggacagc tttttcaggc cggagacgcg caagaacgtt tttccattca
      841 gtctatttcc aaagtgctga gtctcgttgt cgccatgcgt cattactccg aagaggaaat
      901 ctggcaacgc gtcggcaaag atccgtctgg atcaccgttc aattctttag tgcaactgga
      961 aatggaacag ggtattccgc gtaatccgtt cattaatgcc ggtgcgctgg tggtctgcga
     1021 tatgttgcaa gggcgactaa gcgcaccacg gcaacgtatg ctggaagtcg tgcgcggctt
     1081 aagcggtgtg tctgatattt catacgatac ggtggtagcg cgttccgaat ttgaacattc
     1141 cgcgcgaaat gcggctatcg cttggctgat gaagtcgttt ggcaatttcc atcatgacgt
     1201 gacaaccgtt ctgcaaaact actttcatta ctgcgctctg aaaatgagct gtgtagagct
     1261 ggcccggacg tttgtctttc tggctaatca ggggaaagct attcatattg atgaacctgt
     1321 ggtgacgcca atgcaggcgc ggcaaattaa cgcgctgatg gcgaccagtg gtatgtacca
     1381 gaacgcgggg gaggttgcct ggcgggtggg actaccggcg aaatctggcg ttggtggcgg
     1441 tattgtggcg attgttccgc atgaaatggc catcgcagtc tggagtccgg aactggatga
     1501 tgcaggtaac tcgcttgcgg gtatcgccgt ccttgaacaa ttgacgaaac agttagggcg
     1561 ttcggtttat taatgcagtc tctcgatcca ctcttcgcgc gtttatcccg ttcaaaattt
     1621 cgctctcgct ttcgtctggg catgaaagag cgtcagtatt gcctggagaa aggcgcgcca
     1681 gtcatcgaac aacatgcggc ggattttgtc gctaaacgtc ttgctccggc attaccggct
     1741 aatgacggta agcaaccccc catgcgcggg catccggtgt ttatcgctca gcacgccacc
     1801 gcgacctgct gtcgcggttg tctggctaag tggcataaca ttccgcaggg tgtttcgtta
     1861 agcgaagaac aacaacgtta tatcgtggcg gtaatctatc actggctggt tgtacagatg
     1921 aatcagccat gagaattgcg gaaaacgata aaatagcatt cccagggtac ctttatttgc
     1981 cgccgctgtt agcgaacatg ctatccttta tgagaattta cttatcacgt tgagataatt
     2041 gccacccgca attgtatgga cagataatta atgcatgtac accccatttc aactttccgt
     2101 ttgttccagg aaggtcatct gctacgtaat agcatcgcta tttttgcgct aaccacgctg
     2161 ttctatttta ttggtgcaga gttacggctg gttcacgaac tttctctttt ctggccgctg
     2221 aatggcgtaa tggcgggggt gtttgcccgc tatgtctggc ttaatcgact gcattactat
     2281 gcgatcagtt atgtggcgat gctggtttat gatgccataa ccaccgaatg gggactggtt
     2341 tcactggtta tcaatttctc caatatgatg tttattgtta ccgtcgcctt actggtcgtg
     2401 cgggataagc gtctggggaa aaataagtat gagccagtaa gtgccttacg gctatttaat
     2461 tactgtctga ttgccgcatt attatgcgct attgtcgggg cgattggttc ggtcagtatt
     2521 gatagtctgg atttctggcc tttgcttgcg gactggttca gtgagcaatt ctcaacgggc
     2581 gtgttgatcg tgccttgtat gctgacgttg gctattcctg gagtgctgcc gcgctttaaa
     2641 gcagaacaga tgatgcccgc catcgagctt attgtgtcgg ttattgcctc ggtagtcatt
     2701 ggcggagcgg ggagtctggc gttcccgctg cctgcattaa tctggtgtgc agtgcgctat
     2761 acgccgcagg taacatgtct gttgaccttt gtcaccggtg cggtggaaat cgtactggtg
     2821 gcaaattcgg tgattgatat ctcggtcggt tcgccgttct ccattccgga aatgttctcc
     2881 gcacgtctcg gtattgccac gatggcgata tgcccaatta tggtttcttt tagcgtggca
     2941 gcgatcaatt tgctaatgaa gcaagttgcg ctgcgagccg actttgattt tctgactcag
     3001 gtttactcac ggtccggtct ttatgaggcg ctgaaaagtc catcgctgaa acagacacaa
     3061 catctgacgg tcatgctgct tgatatcgac tatttcaaaa gcattaacga taactatgga
     3121 catgaatgtg gcgataaagt gttaagcgtg tttgcccggc atattcagaa gattgtcggt
     3181 gataaggggc tggtggcgcg catgggcggc gaagaatttg ctgttgcagt gccgtcggtg
     3241 aatcctgtcg atggtctgct aatggcggaa aaaatccgta aaggcgttga actgcaacca
     3301 ttcacctggc aacaaaaaac gctttatctc acggtaagta ttggcgtcgg tagtggtcgc
     3361 gcatcgtacc gaacgctgac cgatgatttt aataaattga tggtcgaagc cgatacgtgt
     3421 ctgtatcgct cgaagaaaga tgggcgcaac cgtaccagca ctatgcgtta cggtgaggaa
     3481 gttgtctgac atctttattc attccgcgcg atttgaatgc aatgtgttct accactttat
     3541 gatccaggaa aaggcgtatt tcctcgatta aaccatgatc cgcgccacac ttttctcata
     3601 aaactgctca ttaatcatca ggagttgatc aaaacagggg ttcggcgtat taaagtttgc
     3661 taacctgtcg caccaattca aacaggttgt atgactaatc agaagggaac ccattgtgaa
     3721 aacactaaat cgtcgcgatt ttcccggtgc acagtatcca gaacgtatca ttcagtttgg
     3781 tgaaggtaac ttcctgcgcg cctttgttga ctggcaaatc gatctcctga atgagcacac
     3841 cgatctgaat tctggcgtgg tcgttgttcg tccgattgaa acttcattcc cgccgtcact
     3901 gagcacgcag gatggtctgt acaccaccat tatccgtggc ctgaatgaga agggggaagc
     3961 ggtcagcgac gcgcgtctga ttcgctcggt taatcgtgaa atcagcgtct acagtgaata
     4021 cgatgaattc ctgaaactgg cgcacaaccc ggaaatgcgc tttgttttct ctaacaccac
     4081 cgaagcgggt atcagctacc atgcgggcga taaattcgat gacgcgccag cggtaagcta
     4141 tccggcaaaa ctgacccgtc tgctgttcga acgctttagc catttcaacg gtgcgctgga
     4201 taaaggttgg atcatcattc cgtgtgagtt gattgactac aatggcgacg cgttgcgtga
     4261 actggtgctg cgctatgcgc aagagtgggc actgccagaa gcatttattc aatggctgga
     4321 tcaggctaac agcttctgtt ctacgctggt ggaccgtatc gttaccggtt atccgcgcga
     4381 tgaagtggct aaactggaag aagaactggg ttatcacgat ggtttcctcg acaccgctga
     4441 acacttttac ctgtttgtga ttcaggggcc gaaatcctta gcgactgagc tgcgcctgga
     4501 caaatatccg ctcaacgtgc tgattgttga cgatattaaa ccgtataaag aacgcaaagt
     4561 ggcaatcctc aacggtgcgc acaccgcgct ggtgccggtg gcgttccagg cagggctgga
     4621 taccgtaggt gaagcgatga acgatgctga aatttgcgca ttcgttgaaa aagccatcta
     4681 cgaagaaatt attccggtac tggatttgcc tcgtgatgaa ctggaatctt tcgccagtgc
     4741 agtcaccggg cgtttccgta acccgtacat taagcatcag ctgctgtcta tcgcgctcaa
     4801 cggtatgacc aagttccgca cccgcatcct gccgcagctg ctggcagggc agaaggcaaa
     4861 cggcacactt ccggcgcgcc ttactttcgc attagcggca ttgattgcgt tctatcgtgg
     4921 tgagcgtaac ggagaaacat atccggtaca ggacgatgca cactggctgg aacgttatca
     4981 gcaactctgg agccagcatc gtgaccgtgt tatcggtacc caggaactgg tagcgattgt
     5041 gttggcagaa aaagaccact gggagcagga cctgacgcaa gtgcctggtc tggtcgagca
     5101 ggttgctaat gacctggatg cgattcttga aaaagggatg cgcgaagctg tacgtccgtt
     5161 gtgctaataa caccgttact tctgaacccg gtttcgaccg ggtttcttat ttcactatat
     5221 tagttacaac atcctatatg cctttatcat taacaggtgt cacatgcagc gcctgttaat
     5281 gttgtaagct tgac
//
LOCUS       DADMEQ010000188         5151 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.190, whole genome shotgun sequence.
ACCESSION   DADMEQ010000188 DADMEQ010000000
VERSION     DADMEQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.190"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            190..447
                     /locus_tag="JNZ05_003805"
                     /pseudo
     CDS             190..447
                     /locus_tag="JNZ05_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="JNZ05_003806"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="JNZ05_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HAY8566836.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /locus_tag="JNZ05_003807"
     CDS             complement(1599..2351)
                     /locus_tag="JNZ05_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HAY8566837.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="JNZ05_003808"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="JNZ05_003808"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAY8566838.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNLISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="JNZ05_003809"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="JNZ05_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3605..4420)
                     /locus_tag="JNZ05_003810"
     CDS             complement(3605..4420)
                     /locus_tag="JNZ05_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY8566839.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            4517..>5151
                     /locus_tag="JNZ05_003811"
     CDS             4517..>5151
                     /locus_tag="JNZ05_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY8566840.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     KVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1375 a         1175 c         1224 g         1377 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagatc atcaatagtc agggaaggaa gttgcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt tcaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatga
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgagat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa atacccggac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aatcatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4621 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4681 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4741 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4801 tagcaaagcc aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca
     4861 aagtgccagt gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac
     4921 tgaacgttat gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc
     4981 caggtttgac taccagaagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt
     5041 ggagaagcaa cggctagcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga
     5101 agaagttcag gccgctttga ctcaggcact actggatctg gaatataccc t
//
LOCUS       DADMEQ010000189         5144 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.191, whole genome shotgun sequence.
ACCESSION   DADMEQ010000189 DADMEQ010000000
VERSION     DADMEQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.191"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            1..253
                     /locus_tag="JNZ05_003812"
                     /pseudo
     CDS             1..253
                     /locus_tag="JNZ05_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..1492
                     /locus_tag="JNZ05_003813"
     CDS             746..1492
                     /locus_tag="JNZ05_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY8566841.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1507..1929
                     /locus_tag="JNZ05_003814"
     CDS             1507..1929
                     /locus_tag="JNZ05_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAY8566842.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGKFQEAVL"
     gene            1987..2343
                     /locus_tag="JNZ05_003815"
     CDS             1987..2343
                     /locus_tag="JNZ05_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566843.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2436..2654
                     /locus_tag="JNZ05_003816"
     CDS             2436..2654
                     /locus_tag="JNZ05_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 domain-containing protein"
                     /protein_id="HAY8566844.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            2656..3018
                     /locus_tag="JNZ05_003817"
                     /pseudo
     CDS             2656..3018
                     /locus_tag="JNZ05_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3018..3683
                     /locus_tag="JNZ05_003818"
     CDS             3018..3683
                     /locus_tag="JNZ05_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566845.1"
                     /translation="MATITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSGEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            3683..4048
                     /locus_tag="JNZ05_003819"
     CDS             3683..4048
                     /locus_tag="JNZ05_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY8566846.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            4446..4658
                     /locus_tag="JNZ05_003820"
     CDS             4446..4658
                     /locus_tag="JNZ05_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY8566847.1"
                     /translation="MSHKCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRTGQTEVAVFTAYEPEE"
     gene            4895..>5144
                     /locus_tag="JNZ05_003821"
     CDS             4895..>5144
                     /locus_tag="JNZ05_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566848.1"
                     /translation="MDITVCTYKPGGIVEKIKKLFSSKYAVIRRDDLSVIVEMDYFPE
                     TPKSIMYRNGRKAIFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT         1407 a         1087 c         1421 g         1229 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgccagatac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gccaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc
      961 gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc
     1021 catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac
     1081 cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt
     1141 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa
     1201 atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat
     1261 tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg
     1321 gacggcatcg ttacgcggtg tggggatgct gacaaatatt aaccatgcag cgatgaatac
     1381 acttctcggc gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct gggtgaattt
     1441 taactgggag agctggcgtc cgaatgttag ccattcgagg gttgttaagt agtttcagga
     1501 ggatttatgg cgaaaccttt tactcccgaa cagcgggaag aactgaagac gcgaattgtg
     1561 gaactcgtgc atcaggacgg tcgggtcacg attcggcagt tgtcagatga aacaggtatc
     1621 agtcgtgcgt ctgtcggtcg cttatgcatg gaactggtcg caagtggtga tgtatataat
     1681 tctggctacg gggtatttcc ctctgaacag gctcataaag actggcaaaa cgcccgcaaa
     1741 aagctatcga gggtaaaggt gaagaaaccg gttgtggttg atcctgacct tatctggtca
     1801 ttaccagacg gagaaatacg ccgctacgac aggcgcctga atataatctg tcgcgagtgc
     1861 cggaagagcg aagctatgca gcgcgtgctg gcgttttatc aggggaaatt tcaggaggcg
     1921 gtactgtgag taaaattagc tatcaggctt caattaccgc tggtattcgc atcaaaggag
     1981 aggagcatgg aaataaaacc agaagatgag ttaagcaata tcgttttatt tccggtaaaa
     2041 gaggatgacc cacgtaatca ggttaatttt ctttatgaac catcggaaag accatattgc
     2101 catcacgcct ctgtccgggt tgacgaaaaa gagcgtcagg tccgctgtaa aatctgcggt
     2161 gcggttgtgg agccgtttga ctggatgctc tctgtggcaa aaagagaaac cagactggca
     2221 gatgatgtaa ggctattgcg ccaggaggaa caggaaaggc ggagaaatat agaaaagctg
     2281 atacagattg agcgtaacgc gaaagcgcgg atacgcaggg cgacaaaatc cagaactgaa
     2341 taattaaatt tagcactgtt aaaaatttaa tccttaaccg gagggatttc tgcaccctca
     2401 aatcatcagg agaccgcccg aaagggcggt aatgaatggt tacattattt agaaaaaaat
     2461 atccgcgaaa gagtagaaca acagaattcc tgtttctcat tctgtttatc gtgttgatga
     2521 caccgatatc cccgctaatt tttgtctgga taatcggaaa aataattgag ccagttattg
     2581 aattgtataa cgacgtggta tgggcgtcat tcaacacact gcacaataaa attaatccgt
     2641 ataaggaaag ctgatatggc actgacgaaa aaacaacgtg cagaactgcg catgaagttc
     2701 ggtggtcgct gcgcttattg cggctgcgaa cttggcgaaa agtggcatgc agaccatgta
     2761 aaaccggtca ttcgttttga tggaaatatg cttcaccagg aacgtgacga tatatccaac
     2821 atggtaccag aatgccaccc atgcaatctg cacaagcatt gcagtagcct agaagattat
     2881 cgacgaatta tcagtgatgg tcgtcgtgaa ttccttgcgt ccgggaaagg caaagcgctg
     2941 gttcgtatgg gattggttga aatgaaatct gacccggttg tgttctggtt tgaaaaatat
     3001 caagaagggg ctacggcatg gccactatta ccaaagagcg actgcaatgg ctggctaaca
     3061 tttctggccg cgatgatatt gacgatatag acggcggtga aattcgtgaa ctggcgctta
     3121 tcgctctggc atcactggaa gcagagcctg tagcggaatg cattgttgaa gatgggggca
     3181 tgtgtattga cgggttcggt gagtatgtgg gtcactcgct gcctgatgga atgcatgagc
     3241 tttatgctgc cccgcgaatg cgacaacctg atggatggaa agccgtagct gtagcgtgga
     3301 aggtgacgtt tactcaagtt gaccaggaat ctaatacgtt cactgctata tattttgaca
     3361 aagcggaagt tgagcgttgg gtacgactgc ataaagcatg tgattttcgg gcagatataa
     3421 caccgcttta cgcagcgccg gcagtgccgg ttgcagtaaa cgacgacatg gcttacgcat
     3481 tccatcacgc actgtcagat tcatcgcttg gctctggtga aatcgaagaa attaaaaccg
     3541 gtttgcgtgc tgcctttgcc aacgtcacta tccaaccaga gccgatagtg ccggatgaaa
     3601 tcgggccaaa cgatagtaat acgtttgatt atgttgatgg ctggaacgcc tgccgcgctg
     3661 ccatgcttaa gggagataaa taatgattaa tcgaaccaaa ctggagcaca ttctcgaata
     3721 tgccaggcag cagaaatgta ttgggcaact ttgtaaaatt ccaccaggag atatggttga
     3781 aatcgtggaa atggccatgc gtaaggctgg caactctccg gtaactctgg ctggctggat
     3841 aagctgtagt gatgcagttc ctgctgaata ttgcgatgtg attcttctcg atgatctcgg
     3901 gaatgtattc cccggttcct gggataaggt tttttgcccc actcgtggcg ggaataagat
     3961 ggcttttgtg gacaaagacg gcgttgaagt agagagctca actcactgga tgccgctacc
     4021 ggaaccaccg caggaggtga atcaatgacc tggcctgagg cattcaccac ggtaggaatt
     4081 gcgatggcgg tggcgctggt ggtgtattcg atttgccgtt ggggttaaca aacaaaaacc
     4141 ccggattgat ggtccggggt ttttgaagga aacaaaacag aaacagcaat tgccgttacc
     4201 tgttgttacc atggcaagta aacgtatctc aggcgagcgc attgcgccgt tctgacgcag
     4261 ataaattagc ctggatagaa ggtgctggca ataaaaaata gcgttttctt atcggtatcg
     4321 gtaagattgc tgcgggtgct tgaggctgtc tgcctcgggc atgccactgt aaggcagaca
     4381 gagaaaagcc ccagttaaca ttacgcgtcc tgcaagacgc ttaacattaa tctgaggccc
     4441 aatctatgtc tcacaaatgt aggttagcct cttacgtgcc gaaaggcaag gagaagcagg
     4501 ctatgaagca gcaaaaggcg atgttaatcg ccctgatcgt catctgttta accgtcatag
     4561 tgacggcact ggtaacgagg aaagacctct gcgaggtacg aatccgaacc ggccagacgg
     4621 aggtcgctgt cttcacagct tacgaacctg aggagtaaga gaccaggcgg gggagaaatc
     4681 cctcgccacc tctcatgtgt caggcatcct caacgcaccc gcactaaacc cgcttcggcg
     4741 ggttttttgt tgcgtgctga atacgcaggg tgaaaaataa ccatatattt gattatatac
     4801 acaacaaaaa ataaaagtca ttgtacttgc acattaaaca atcaaatata cggcgtgaaa
     4861 taaatatttt tcagattaat atttttgtct ctatgtggat ataaccgttt gtacttataa
     4921 acctggaggc atcgtggaaa aaataaagaa actatttagt agcaaatacg cagtcatacg
     4981 tcgtgatgac ctgtcagtta tagtcgaaat ggattacttc cctgaaaccc caaaatcaat
     5041 aatgtatcgt aatggtcgaa aggcaatttt tttaccgatg agggtaagtg acattatggg
     5101 aaatgataaa ctgctggatg aattgcgagt cagagcatcc tgtt
//
LOCUS       DADMEQ010000190         5128 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.192, whole genome shotgun sequence.
ACCESSION   DADMEQ010000190 DADMEQ010000000
VERSION     DADMEQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.192"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..238
                     /locus_tag="JNZ05_003822"
     CDS             <1..238
                     /locus_tag="JNZ05_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566849.1"
                     /translation="WAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWL
                     GALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            278..818
                     /locus_tag="JNZ05_003823"
                     /pseudo
     CDS             278..818
                     /locus_tag="JNZ05_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            885..2303
                     /locus_tag="JNZ05_003824"
     CDS             885..2303
                     /locus_tag="JNZ05_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAY8566850.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2284..2754
                     /locus_tag="JNZ05_003825"
     CDS             2284..2754
                     /locus_tag="JNZ05_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HAY8566851.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(2743..3663)
                     /gene="yedA"
                     /locus_tag="JNZ05_003826"
     CDS             complement(2743..3663)
                     /gene="yedA"
                     /locus_tag="JNZ05_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HAY8566852.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGEIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            3836..4753
                     /locus_tag="JNZ05_003827"
     CDS             3836..4753
                     /locus_tag="JNZ05_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HAY8566853.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            4832..5014
                     /locus_tag="JNZ05_003828"
     CDS             4832..5014
                     /locus_tag="JNZ05_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HAY8566854.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
BASE COUNT         1253 a         1227 c         1463 g         1185 t
ORIGIN      
        1 gtgggctttc tatacatggt gtggctattt cgtctgggca atggcgcgat acatctgggt
       61 gatgagccgg atccccgatg cccccgtgtc gggatttgaa agtgatttag gatctaccgc
      121 ggggaagtgg ttaggagcat tggtcggatt tctgtttatg gccctggttg gtgccttgtt
      181 aggcggcatt gcatggtaca cccgacctcg tcccgcacgt tgcagacgct atgaataaaa
      241 actgctaagg ttgcgactct ttggcaacgg agcgcgtatg gagttacaac actggcaggc
      301 acagttcgag aactggttga aaaaccatca tcaacatcag gacgcggcgc atgacctgtg
      361 tcattttcgc cgtgtctggg ctacggcaca gaaactggcg gcagatgacg atgtcgatat
      421 gctggtgatt ttaaccgcct gttattttca cgatatcgta agcctggcga aaaatcatcc
      481 gcaacggcag cgttcttcaa tcctggcggc agaagagacg cgtcgcctgc tccgtgaaga
      541 gttcgtgcaa tttccggcgg agaaaattga ggccgtttgt catgccattg ccgctcacag
      601 tttcagcgcg caaatcgccc ccttaacaac ggaagctaaa atcgtccagg atgcggatcg
      661 gctggaagcc ctgggggcga ttggcctggc gcgcgtattt gccgtttcag gggcattgag
      721 cttggcgctg tttgatggcg aagctccgtg ccgaactggc gggggagaat gaaggtatcg
      781 atcacaaggt gatagatgcg ttttcacccg ctggctgagc gcgtggccct aaacggcagt
      841 aattatgtta acctgtcggc catctcagat ggccggttaa atctatgcag gaaaatatat
      901 cagtaaccga ttcatacagc accgggaatg ccgcacaggc aatgctggag aaactgctgc
      961 aaatttatga tgttaaaatg ctggtggcgc agcttaatgg tgtgggtgag aatcactgga
     1021 gcgcggcaat tttaaaacgt gcgctggcga atgactcggc atggcaccgt ttaagtgaga
     1081 aagagtttgc ccatctgcaa acgttgttac ccaaaccacc ggaacatcat ccgcattatg
     1141 cgtttcgctt tatcgatcta tttgccggaa ttggcggcat ccgtcgtggt tttgaatcga
     1201 ttggtggaca gtgcgtgttt accagcgaat ggaacaaaca tgcggtacgc acttataaag
     1261 ccaaccatta ttgcgacccg gcgacgcatc attttaatga agatatccgc gacatcaccc
     1321 tcagtcatca agaaggcgtg agtgatgagg cggcggcgga acatattcgt caacacattc
     1381 ctgaacacga tgttttactg gccggtttcc cttgtcagcc attttcactg gctggcgtat
     1441 cgaaaaagaa ctcgctcggg cgggcgcacg gttttgcctg cgatacccag ggcacgctgt
     1501 tctttgatgt ggtgcgcatt atcgacgcgc gtcgtccggc gatgtttgtg ctcgaaaacg
     1561 tcaaaaacct gaagagtcac gataagggaa aaacgttccg catcatcatg cagacgctgg
     1621 acgaactggg ctatgacgtt gccgatgcag aagataacgg gccggacgat ccgaaaatca
     1681 tcgatggtaa acatttcctg ccgcagcacc gtgaacgcat cgtgctggtg ggttttcgtc
     1741 gcgatctgaa tctgaaagcc gattttactc tgcgtgatat cagcgaatgt ttccctgcac
     1801 agcgagtgac gctggcgcag ctgctggacc cgatggttga ggcgaaatat atcctgacgc
     1861 cggtgctgtg gaagtacctc tatcgatatg cgaaaaaaca tcaggcgcgc ggtaacggct
     1921 tcggttatgg aatggtttat ccgaacaatc cgcaaagcgt cacccgtacg ctgtctgctc
     1981 gttattacaa agatggcgcg gaaattttaa tcgatcgcgg ttgggatatg gccacgggtg
     2041 agaaagactt tgacgatccg ctgaatcagc aacatcgtcc acgtcggtta acgcctcgtg
     2101 aatgcgcgcg cttaatgggt tttgaagcgc cgggagaagc gaaattccgc attccggttt
     2161 cggacactca ggcctatcgc cagttcggca actcggtggt cgtgccggtc tttgccgcgg
     2221 tggcaaaact gcttgagcca aaaatcaaac aggcggtggc gttgcgtcag caagaggcac
     2281 aacatggtcg acgttcacga taaggccacc cgcagcaaaa atatgcgcgc gattgccacg
     2341 cgtgatacgg cgattgagaa gcgcctcgcc agtttgttaa ccgggcaggg ccttgcgttt
     2401 cgcgttcagg acgccagtct gcccggacgc ccggattttg tcgttgatga atatcgctgc
     2461 gtgatattta cccacggctg cttctggcat catcatcact gctatctgtt taaagtgcct
     2521 gcgactcgaa ctgagttctg gctggagaag ataggtaaaa atgttgaacg cgatcgccgc
     2581 gatatcagtc gcttgcagga gctcggctgg cgcgtactga ttgtctggga gtgtgcgtta
     2641 cgtaggcgcg agaagctgac ggatgcggcg cttaccgagc gtctggaaga gtggatctgc
     2701 ggcgaaggtg ccagcgcgca gatcgacacg caggggattc atttactcgc ttgatgcatc
     2761 ctgaataact ggcgcgacaa cgggtttggc cgggaagaga tattttccca acgtgaccag
     2821 taccaccgcg aaaacaatta cgccgagcgc cagccattca atcttcgaca gcgtttcgcc
     2881 acccagtccc gtacccagca agaccgcgac caccgggtta acgtaggcgt agctggtggc
     2941 gagagccgga ctgacattac ggattaaata catataagcg ttaatggcga taatcgaacc
     3001 aaacagcgcc agatagccga ccgcaaggaa gcctgacagg gaagggagcg ccgtcagttt
     3061 ttcacccgca atcatcgatg cgatcattaa caccacgcct gccgccagca tctcaatctc
     3121 acccgccatc atccctacag gtaaggtaat gcgcgagcca taaactgagc caaacgccca
     3181 gctaatcgag ccgattaaaa tcagaatcgc gccccacgga ttgccgctta aatttccacc
     3241 gctattgagc atgatgattc cggcaagccc aatggcaata cccacccatt ccagtttgcg
     3301 cgttttaatg ccaaacaggc gactgaagca cagggtaaag aggggcacgg ttgcaatcac
     3361 tacggcggcg atgccggaag gaacattttg atgttcggca accgtcacca tgccattacc
     3421 gacagccagc aataacaggc caatcagcgc ggcattgagc agcggacgta gcgggggaag
     3481 tttgtgtccg cgcagtagca aaaatgccag caataaaata ccggctgcca ggaatcgaac
     3541 gcccgccatc attaacggag gccagcttcc cacgccaatc cgaatgacaa aataggttga
     3601 gccccaaatg atatacaacg caaacagagc gccaaaaagc ggtaacaact ggcggaaacg
     3661 cataatccct cacggcggaa ataaaaaggt ggttcatagt aaacgtgaaa atcattctgc
     3721 tggcgagaga tataattgca cttgattgtt aaataaatgt tgacctgtga agcaagtatc
     3781 agagctgccg tttttgcttc atacttacac ccttcagcaa taaaaatgag agggaatgct
     3841 tttggccggg agtagtttac tgacgttgct cgatgatatc gccacactgc tggacgatat
     3901 ctctgtgatg ggcaaactgg cggcgaagaa aaccgccggt gtattagggg atgatttatc
     3961 gctcaatgcg caacaagttt caggcgtgcg ggccaaccgg gaacttcccg tggtctgggg
     4021 cgtggcgaaa ggatcgctga tcaataaagt gattctggtg ccgctggcgc tgatcatcag
     4081 tgcgtttatc ccgtgggcga ttacgccact gttgatgatt ggtggcgcgt ttctctgctt
     4141 tgaaggggta gagaaagtgc tgcatatgct ggaggcgcgt aaacataaag aagatccagc
     4201 gcagagccag cagcgtctgg agaagctggc ggcgcaggat ccgctgaagt ttgaaaagga
     4261 caaaattaaa ggggcgattc gtaccgattt tattttgtct gcggaaatcg tcgccatcac
     4321 gctggggatt gtggcagagg cgccgttgct taatcaggtg ctggtgcttt caggcatcgc
     4381 gctggtagtg accgtgggcg tctacggtct ggtaggggtt atcgttaaga ttgatgacct
     4441 agggtattgg ctggcggaaa aatccagcgc gctgatgcag gcattaggta aagggttatt
     4501 gattatcgcg ccttggctga tgaaagcatt atcgattgtc ggcacgctgg cgatgttcct
     4561 cgtcggcggc gggattgtgg tacatggtat tgcgccgctg catcacgcta ttgaacatat
     4621 cgccgggcag caaagtgcag tggtggcgat gatattaccg actgttttaa atctgattct
     4681 tggatttatc atcggcggca tcgtggtgct gggagtgaaa gcagtagcga aaatgcgcgg
     4741 tcaggcacat taatactcgc actggctatt atgcaaaatt cgccatcttc aactaaggtg
     4801 aacaagtttc actcgaaaaa aggaggcgag tatgagcttt atggttagtg aggaagttac
     4861 ggttaaagag ggcggcccgc gaatgattgt cactggatac tccagcggta tggttgagtg
     4921 tcgttggtat gacggttacg gggtcaagcg ggaagctttt catgaaaccg atcttgttcc
     4981 gggggagggg agtcgttctg cggaagaagt ttgaaagggt atcattaaca ccaggccgga
     5041 tcacatccgg cctggtgtcc caatgcctgc aatgaatgat agtcgcgtct tatcatgcca
     5101 ccattataaa attatcgctg ttttgcgg
//
LOCUS       DADMEQ010000191         5116 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.193, whole genome shotgun sequence.
ACCESSION   DADMEQ010000191 DADMEQ010000000
VERSION     DADMEQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.193"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..366)
                     /locus_tag="JNZ05_003829"
     CDS             complement(<1..366)
                     /locus_tag="JNZ05_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566855.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLIIKNSTIHGMIYSECMTT"
     gene            892..1866
                     /gene="hemB"
                     /locus_tag="JNZ05_003830"
     CDS             892..1866
                     /gene="hemB"
                     /locus_tag="JNZ05_003830"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAY8566856.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1972..2823)
                     /gene="tauD"
                     /locus_tag="JNZ05_003831"
                     /pseudo
     CDS             complement(1972..2823)
                     /gene="tauD"
                     /locus_tag="JNZ05_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
     gene            complement(2820..3647)
                     /gene="tauC"
                     /locus_tag="JNZ05_003832"
     CDS             complement(2820..3647)
                     /gene="tauC"
                     /locus_tag="JNZ05_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HAY8566857.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVQALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(3644..4411)
                     /gene="tauB"
                     /locus_tag="JNZ05_003833"
     CDS             complement(3644..4411)
                     /gene="tauB"
                     /locus_tag="JNZ05_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HAY8566858.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVQERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4424..>5116)
                     /gene="tauA"
                     /locus_tag="JNZ05_003834"
     CDS             complement(4424..>5116)
                     /gene="tauA"
                     /locus_tag="JNZ05_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8566859.1"
                     /translation="VPIEVFLLASKLGNSEALVVKKTISKPEDLIGKRIAVPFISTTH
                     YSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDGAYVWAPAINALEKDGKVLTD
                     SEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAIDAQQPYIANPDAWLKQPENIS
                     KLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNKAIIDTAQFLKEQGKVPAVAN
                     DYSQYVTSRFVQ"
BASE COUNT         1241 a         1408 c         1273 g         1194 t
ORIGIN      
        1 agttgtcatg cactcggaat aaatcatacc gtgaatagtg ctgtttttga ttatcagact
       61 attggcgttg gtattggtag aaataccgtc gtccagatag taggtagaaa tcacgccgtt
      121 aacggtagcg ttattaatga ccgggtagat atcgccgttg taatagctat cagcggcata
      181 gttgttccag cctacataac catcatagta aacgtcatca gcataggtgg cgtcgttgta
      241 gtgatggaaa gtattgtaag tttgaccaga aatatcgttg gtcgctgcat tagcctgaga
      301 ggtgatagcc agagtgcaag ccaatgctaa ttgtgatact acaagtttct ttttccagga
      361 gtgcatttgt catccctcct cagggacgta ataagttgat ccatcaatta tcaatgcata
      421 gaaaacatat tatgtttcgc gctaacgatt atgctgcgtt acagacgaat ggttcaatta
      481 ttctttcccc taagtccgaa tgtggacata tataatttat ggagaaaaac acgcatcaaa
      541 ttgataaaag gacgatattt cagcatgttg cagataatgt tatttttgat gcatacgcgt
      601 tataaatatc aaaaacacaa accagataaa tatcttatca atatacaaca aagtttgaat
      661 tattcaattt atagcagcac aaccaaacgc caaaaaaaat aataaccaag agctataaat
      721 aaagattaaa aatattttca gtgaatatga aattattgct atttctatta caaagatgtc
      781 ctggtctctc tttcattctc ttcttgtcaa catcgcgaca actttcgtaa aacactcctg
      841 ccccgcttca ggtatactgt gcaccttaat tccacaaaca tcaggcagac catgacagac
      901 ttaatccaac gccctcgtcg cctgcgcaaa tctcctgcgc tgcgcgctat gtttgaagag
      961 acaacactta gccttaacga cctggtgttg ccgatctttg ttgaagaaga aattgacgac
     1021 tacaaagccg ttgaagccat gccaggcgtg atgcgcattc cagagaaaca tctggcacgc
     1081 gaaattgaac gcatcgccaa cgccggtatc cgttccgtaa tgacttttgg catctctcac
     1141 cataccgatg aaaccggcag cgacgcctgg cgggaagatg gactggtggc gcgtatgtcg
     1201 cgcatctgca agcagactgt gccagaaatg atcgtcatgt cagacacttg tttctgtgaa
     1261 tacacttctc acggtcactg cggtgtgctg tgcgagcatg gcgtcgacaa cgacgcgact
     1321 ctggaaaatt taggcaagca agccgtggtt gcagctgctg caggcgcaga cttcatcgcc
     1381 ccttccgccg cgatggacgg ccaggtacag gcgattcgtc aggcgctgga cgctgcaggc
     1441 tttaaagata cggcgattat gtcgtattcg accaagttcg cctcttcctt ttatggcccg
     1501 ttccgtgaag ctgccggaag cgcattaaaa ggcgaccgca aaagctatca gatgaaccca
     1561 atgaaccgtc gcgaggcgat tcgtgagtca ctgctggatg aagcccaggg cgcagactgc
     1621 ctgatggtta aacctgctgg agcgtacctc gacatcgtgc gtgagctgcg tgaacgtact
     1681 gaattgccga ttggcgcgta tcaggtgagc ggtgagtacg cgatgattaa gttcgccgcg
     1741 ctggcgggtg ctatagatga agagaaagtc gtgctcgaaa gcttaggttc aattaagcgt
     1801 gcgggtgcgg atctgatttt cagctacttt gcgctggatt tggctgagaa gaagattctg
     1861 cgttaatttt tttcctctca gaaaggggga gttaacctgt tgaaagtacg gtgaacgccc
     1921 cctcacccta accctctccc caagggggcg agggaaccgt ccacactcgt atcaccccgc
     1981 ccgataaaac ggtttatccc caaggatcgt cgcccgatgc ataatccgcc gctgtggcag
     2041 gtaatcggca ttggcatagt gctgagtcac gcggttatcc caaatcgcaa tatcattcgg
     2101 ttgccagcgc caacgcacct gaaactccgg tttagtgata tgggcgaata aaaagcctaa
     2161 caaggcttcg ctctctttct cgctgacatc gacaattcgc gtagtaaagc cttcattcac
     2221 aaacagcgcc tgtttaccgc tcaccggatg cgttcgcacc accggatgta gcaacggagg
     2281 atttttcgcg accgcctcgc gccagcgttg atgttcctcc tcggttttgc ggtatttgta
     2341 ttccgggaat gatttacgga aatcatgctc cgcacgcagc cagctcagca gctggcggaa
     2401 gggaacagag agcgcctcat aggccgcaat accgctggtt cagagcgtat caccgccggt
     2461 cgaaggtaac tctttagctg ccagaatcgc ccctgcgggt ggcgtttcaa taaatgtcac
     2521 atcggtatgc cagttgtcgt tatccggcgg attatcgtta tgggtatcca gcacgatgat
     2581 ctcgtcaacc ccttcggcat gagggtaaac agggtgaata tgcagttcgc caaaacgctg
     2641 ggccagcgcg cgttgctgct gcggcgtaat agcttgatcg cgcagaaata ccacctgatg
     2701 gcgcagcacc gcatggtaaa gctgttcaaa ctgattatcg cttaacgggc gcgtcaggtc
     2761 agcacccgaa atttgtgcgc cgatatgcgg ccccagcggg gtaatgctca gacgttcact
     2821 cattgtactt ctccatgcca gggcgtcaga cggcgctgta acgcgcgcag acccagttct
     2881 aaaagaaagg cgataatcgc aatcaccgcg atccccgcca gtaccacgtc agtcgcgaga
     2941 aattcacccg ctgactgaac cataaatcct aaaccgcgtg tcgcggcaat cagctccgcc
     3001 gctaccagcg tagaccagcc cacacccaga ccaatgcgta atccggtgag gatttccggc
     3061 agcgcgccgg gcaaaatgac aaaccacagc acctgcgcac ggctggcacc cagcgactgg
     3121 gcggcacgaa tgcgaacctg ctgcgcgctt ttcacccctg ccagcgccga catcgccacc
     3181 ggcgcaaaaa tcgctaaata gatcagtaag atcttcgagg tttcaccaat accaaaccag
     3241 atcaccatca gcggcaaata agccagcggt ggcaccggac gataaagttc gattaccgga
     3301 tccagaatgc cgcgcaccgt cgggcttagc cccatggcga tcccgaccgg aataccgatc
     3361 accaccgccg cgagtagcgc ctgcacaatg cgcgtcaggc tggctgccag atgctgccac
     3421 agcgtggcgt ccataaatcc ctgcggtccg gcaatggtga gcagttttgc cagtacttgt
     3481 tgcggcggcg gcaaaaacag cgggctaatc agttgcagcg ccgccaccgt ccaccataca
     3541 gtgagtaaaa ccgctaacgt gccaatgctt aaggtcacct gacgcgagag cggccagcgc
     3601 catttcagcc gccgcgaatg cagtttttca ttaatgagca cactcatgag aacgcctccc
     3661 gttgctcaaa tacgcggctt aaaacatatt cgcgcatggc gatgaattgt ggatcggact
     3721 tgatgctgcg gctcgactct cccgcaacaa agcggcgagc aaagttgagc ggcagccgct
     3781 cctgcacacg gccagggccg gatgaaagca gaaccagttc agtcgccata aacaccgctt
     3841 cttctatatc gtgggtaatc aacagcacct gcttgcccgt ctcctgccag agtttcagca
     3901 gcagagtttg catctggtcg cgggtgaagg cgtccagcgc accaaacggt tcatcgagta
     3961 ataacagctg gggattcgcc gccagcgcgc gggcaatccc cacccgctga cgttgaccat
     4021 cggaaagctg ccagatatag cgtttttctg cgccttccag ccccactttt ttcagcatct
     4081 ggtgcgcgat ttccagtcgc tgcattttct ctatacctgc caattgcagg ccgaacgcca
     4141 cgttgtcctg tacattgcgc cacggtagca gcccttcatt ctgaaaaact acgccacgct
     4201 ctgctcccgg tccctcaata cgcttacccg ccagttgaat gctgccatgc tgataaggca
     4261 caaaaccggc aatcagattc agcagggtgg ttttaccgca gccggacggc cccagcacca
     4321 ccagtagctc gccgctttcc agcgtcaggt tgatatcttc cagtgccggt ttgccgccat
     4381 aatcggcata gagatgagag atttgcagca tctgcgcctc cttttattgc acgaagcgcg
     4441 aggtaacgta ctggctgtaa tcattcgcta cagccgggac cttgccctgc tctttcaaaa
     4501 actgcgcggt gtcgatgatt gctttgttca ccggtccggt cagttctgcc gtttgttgct
     4561 gcggcgtcag ataggtattt cccttcacca gccccggaac gtcaccttca ggcacgccgc
     4621 ttaaacgcgc cagtttgctg atgttttccg gctgtttcag ccacgcgtca gggttagcaa
     4681 tgtacggttg ctgtgcatcg atggcgcttt tggcgaacgc tttcacgacc tcaggatgtt
     4741 tctcggcaaa atctttgcgc accacccaga cgtccagcgt tggcgcaccc cactgcccga
     4801 cctgttcaga atcggtcagc accttgccgt ctttttccag ggcgttaatc gccggtgccc
     4861 agacataagc accatcaata tcaccccgct gccatgcggc gataatcgcg ggcggctgca
     4921 ggttcacaat ctccactagc cctggtttaa tcccccagtg tttcagcgcc gccagcaggc
     4981 tgtagtgggt ggtggagata aacggtacgg caatgcgttt accaatcaaa tcttcgggtt
     5041 tgctgatagt tttctttacc accagcgctt cggagttacc cagttttgac gccagcaaga
     5101 agacttcaat cggcac
//
LOCUS       DADMEQ010000192         4992 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.194, whole genome shotgun sequence.
ACCESSION   DADMEQ010000192 DADMEQ010000000
VERSION     DADMEQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.194"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            91..1206
                     /gene="adrA"
                     /locus_tag="JNZ05_003835"
                     /gene_synonym="dgcC"
     CDS             91..1206
                     /gene="adrA"
                     /locus_tag="JNZ05_003835"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HAY8566860.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(1223..2032)
                     /gene="proC"
                     /locus_tag="JNZ05_003836"
     CDS             complement(1223..2032)
                     /gene="proC"
                     /locus_tag="JNZ05_003836"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAY8566861.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRVQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            2152..2610
                     /locus_tag="JNZ05_003837"
     CDS             2152..2610
                     /locus_tag="JNZ05_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAY8566862.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2793..3317
                     /gene="aroL"
                     /locus_tag="JNZ05_003838"
     CDS             2793..3317
                     /gene="aroL"
                     /locus_tag="JNZ05_003838"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HAY8566863.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPNQV
                     ISEIRSALAQTINC"
     gene            3367..3558
                     /gene="yaiA"
                     /locus_tag="JNZ05_003839"
     CDS             3367..3558
                     /gene="yaiA"
                     /locus_tag="JNZ05_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HAY8566864.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            3816..4493
                     /locus_tag="JNZ05_003840"
     CDS             3816..4493
                     /locus_tag="JNZ05_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HAY8566865.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            4565..4849
                     /gene="ppnP"
                     /locus_tag="JNZ05_003841"
     CDS             4565..4849
                     /gene="ppnP"
                     /locus_tag="JNZ05_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HAY8566866.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT         1213 a         1186 c         1315 g         1278 t
ORIGIN      
        1 gctaacgggt gagctacgaa agtggctcac cctaaatttc atacttctgc ctttagcccc
       61 gtctctataa tttgggaaaa ttgtttctga atgttcccaa aaataatgaa tgatgaaaac
      121 tttttcaaaa aagcggcggc gcatggggag gaacctcctt taactcctca aaacgaacat
      181 cagcggtccg ggctgcgctt cgcccgtcgc gtcagactac cccgtgcggt tggcctggct
      241 ggcatgttct taccgattgc ttcaacgctg gtttcgcacc cgccgccggg ctggtggtgg
      301 ctggtgttgg tcggctgggc gttcgtctgg ccgcatttag cctggcagat agcgagcagg
      361 gccgtcgatc cgcttagccg ggaaatttac aacttaaaaa ccgatgcagt attagcggga
      421 atgtgggtag gcgtaatggg cgtaaacgtg ctgccttcca ccgctatgtt gatgattctg
      481 tgtctgaatt tgatgggggc aggcggcccc cgtctgtttg tcgcgggtct ggtgttgatg
      541 gtggtttcct gccttgtcac cctcgagctt acgggcatta ccgtgtcgtt caatagtgcg
      601 ccgctggaat ggtggctctc ccttcccatt attgtcgttt atcctctgct gtttggctgg
      661 gtcagctacc agacggcaac caaactggcg gaacataaac gcaggttgca ggtcatgagt
      721 actcgcgacg gcatgacggg cgtgtataac cgacgtcatt gggaaactat gttacgcaat
      781 gaatttgata actgtcggcg gcataatcgc gatgcaacgt tactgattat cgatatcgac
      841 catttcaaga gcatcaacga tacctggggt catgatgtgg gcgatgaagc gattgtggcg
      901 cttacccgac agttacaaat taccctgcgc ggtagcgatg tgattggtcg gtttggcggc
      961 gatgagtttg cagtaatcat gtccggtacg ccagctgaga gcgccattac cgccatgtta
     1021 cgggtgcgtg aagggctaaa cacgttacgc ttgccgaata cgccacaggt aactttacgg
     1081 attagtgtgg gggttgcgcc gctgaaccca caaatgagtc actatcgtga gtggttgaaa
     1141 tcggcagatt tggcgcttta caaagcaaag aaagccggac gtaaccgcac cgaagtggcg
     1201 gcctgacgtc cggcgaaagt catcaggatt tgctgagttt ttctgatttt tccatacact
     1261 tcgtcatcgc ttcgatcact gcggcacgga agcctttctc ttccagtacg cgtaccgctt
     1321 caatggtggt acctcccggt gagcagacca tatctttcag tgcccccgga tgttctcccg
     1381 tttccagcac catttttgcg gaacccatta ccgcctgagc ggcaaattta tacgcctgga
     1441 cgcgtggcat cccgcccagc acggcggcgt cggccatcgc ttcgataaac ataaatacgt
     1501 aggctggcga agaaccgctc acaccgacca ccgggtggat catcggctca gcaattactt
     1561 ccgcttcgcc aaagcagcgg aaaatattca gcacatcagc ggtatcttct ggggttacca
     1621 gcgcgtttgg cgttacggag gtcatcccgg cattaaccag cgcgggagtg ttcggcatgg
     1681 cgcggataat tttccggtca tggcccagcg cgcgggcaag ctggtcgagc gtgacacctg
     1741 cagcaataga aacgaccaga gagtctttat tcaggctgga ggtgatttcg ctaagcactt
     1801 taatcatgat gccaggttta acggcagcaa aaatgatgtc ggcgatttgc gccacttctt
     1861 gcgccgattc tgcggcgttg atgccgaact ggtcatgcag ggcggcgact ttatccgggg
     1921 agggggtgta tacccagatt tgccctggaa gcacctgacc gctggcaatc agaccgccga
     1981 gaatggcttt tcccatattg ccgcagccaa taaaaccgat tttcttttcc attgcctcac
     2041 tcctgccgtg aaattcattg ttttgataat cgctggcaga agcataaaca gaactatgcc
     2101 ggaaggcaaa agcgcgacac aatagaggat tacccaacaa aggatgactt tatgacaatt
     2161 tgggtggatg ccgacgcgtg tcccaatgta attaaagaga ttttgtatcg cgcggcggaa
     2221 cgtatgcaga tgccgctggt actggtagca aaccagagtt tacgcgtgcc gccatcgcga
     2281 tttattcgta cgctgcgcgt cgcggcaggt ttcgacgttg ccgataacga aattgtccgg
     2341 cagtgtgaag cgggcgattt ggtgatcacc gcagatatac ctttggctgc tgaagccatc
     2401 gagaaaggcg cagcggcgct taatccgcgc ggcgaacgtt acacgccagc gaccattcgt
     2461 gagcgcctga cgatgcgcga ttttatggat accttacgtg ccagtgggat ccagaccggc
     2521 ggaccagata gcctttcaca acgtgaccgc caggcctttg ccgcggagct ggagaagtgg
     2581 tggctggaag tgcaacgtag tcgtggctaa atgtaattta ttatttacac ttcattcttg
     2641 aatatttatt ggtatagtaa gggatgtatt gagattttca ctttaagtgg aattttttct
     2701 ttacaatcga aattgtacta gtttgatggt atgatcgcta ttctcatgac accggctttc
     2761 gccgcattgc gacctattgg ggaaaaccca cgatgacaca acctcttttt ctgatcgggc
     2821 ctcggggctg tggtaaaaca acggtcggaa tggcccttgc cgattcgctt aaccgtcggt
     2881 ttgtcgatac cgatcagtgg ttgcaatcac agctcaatat gacggtcgcg gagatcgtcg
     2941 aaagggaaga gtgggcggga tttcgcgcca gagaaacggc ggcgctggaa gcggtaactg
     3001 cgccatccac cgttatcgct acaggcggcg gcattattct gacggaattt aatcgtcact
     3061 tcatgcaaaa taacgggatc gtggtttatt tgtgtgcgcc agtatcagtc ctggttaacc
     3121 gactgcaagc tgcaccggaa gaagatttac ggccaacctt aacgggaaaa ccgctgagcg
     3181 aagaagttca ggaagtgctg gaagaacgcg atgcgctata tcgcgaagtt gcgcatatta
     3241 tcatcgacgc aacaaacgaa cccaaccagg tgatttctga aattcgtagc gccctggcac
     3301 agacgatcaa ttgttgattt tagagcgcct atacttaacg ttcatcccgt gaaataagga
     3361 agaacgatgc caacgaaacc gccttatcct cgtgaagcat atatagtgac gattgaaaaa
     3421 ggaaagccag gacagacggt aacctggtac caactcagag ccgatcatcc taaaccagac
     3481 tcgttgatca gtgaacatcc gacggctcag gaagcgatgg atgcgaaaaa acgctatgag
     3541 gaccctgaca aagagtgacc gcatcagact gctcggaagg gattctgagt gccactacaa
     3601 gggatctgcg tcacattttt cataattcat gtttttctaa taattagaat attaaacaat
     3661 aacattccat tactggaatc atttggaatc tttacattat gccgtgcacg tctgctgcta
     3721 cgctttttgt catttgtagc acaagtaagt gtcagcagtg gtgcttcaca cttgcccggt
     3781 aattaacgac gaaagaaaag taaggtggat gaacaatgag tgcgtcgttg gcgatcctga
     3841 ccatcggcat tgtacctatg caggaggttt tgccgctcct gacggaatac attgacgaag
     3901 ataatatttc ccatcatagc ctgctgggga agttaagtcg tgaagaagtg atggcggagt
     3961 acgcgccaga agcaggcgaa gacaccattc tcacattatt aaatgacaac cagctggccc
     4021 atgtttcgcg tcgtaaagtg gagcgagatc tgcaaggtgt ggttgaagtg ctcgataatc
     4081 agggttatga cgtcatttta ttaatgagta cagcaaacat tagtagtatg actgcgcgta
     4141 atacgatctt tcttgagccg tcgcgaatat tgcctccact ggtttcctct attgttgaag
     4201 atcatcaggt gggggttatc gttccggttg aggagatgct gcccgttcag gcgcaaaaat
     4261 ggcaaatttt gcagaaatcg ccggtatttt cattgggtaa ccccattcat gattcagaac
     4321 aaaaaatcat tgatgctggg aaagaattac tggcaaaagg ggctgatgtc atcatgctgg
     4381 attgtttagg atttcaccaa cgtcatcgcg atttactgca aaaacagctc gatgttcctg
     4441 tcttgctctc taacgtattg attgcacggc tggctgcgga attactggtg taattttgcg
     4501 tgacagccag cgcctctggc ccctatagtg aagtagatgt tcaactacca gacagggcca
     4561 gtttatgctt caaagtaatg agtacttttc cggcaaagtg aaatcgatcg gcttttccag
     4621 cagcagcact ggtcgcgcca gcgtgggtgt tatggttgaa ggcgaataca ccttcagcac
     4681 cgctgagccg gaagagatga cggtaatcag tggcgcgctg aatgtgttac tgcctgacgc
     4741 gaccgactgg caagtgtatg aagccggttc ggtgtttaat gttcccggtc acagtgagtt
     4801 tcatcttcaa gttgccgaac ctacctctta tctgtgccgc tatctgtaat tcctcgcctt
     4861 ccccttgaac gggagggcat ttttctgaaa tatcctctct ttaacccata ataatatttc
     4921 ctttgctgcg attttttcaa tttccgatat attcataatt catcaaggtt gatataaata
     4981 tcagtgaaga tc
//
LOCUS       DADMEQ010000193         4950 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.195, whole genome shotgun sequence.
ACCESSION   DADMEQ010000193 DADMEQ010000000
VERSION     DADMEQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.195"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            1..611
                     /locus_tag="JNZ05_003842"
                     /pseudo
     CDS             1..611
                     /locus_tag="JNZ05_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
     gene            632..1292
                     /gene="csiR"
                     /locus_tag="JNZ05_003843"
                     /pseudo
     CDS             632..1292
                     /gene="csiR"
                     /locus_tag="JNZ05_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
     gene            complement(1293..1742)
                     /gene="kbp"
                     /locus_tag="JNZ05_003844"
     CDS             complement(1293..1742)
                     /gene="kbp"
                     /locus_tag="JNZ05_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HAY8566867.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(1826..1984)
                     /locus_tag="JNZ05_003845"
     CDS             complement(1826..1984)
                     /locus_tag="JNZ05_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HAY8566868.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            2167..2466
                     /locus_tag="JNZ05_003846"
     CDS             2167..2466
                     /locus_tag="JNZ05_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY8566869.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            2476..3000
                     /gene="ygaP"
                     /locus_tag="JNZ05_003847"
     CDS             2476..3000
                     /gene="ygaP"
                     /locus_tag="JNZ05_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HAY8566870.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKIPWNQRA"
     gene            complement(3047..3451)
                     /gene="stpA"
                     /locus_tag="JNZ05_003848"
     CDS             complement(3047..3451)
                     /gene="stpA"
                     /locus_tag="JNZ05_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HAY8566871.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            4119..4556
                     /gene="alaE"
                     /locus_tag="JNZ05_003849"
     CDS             4119..4556
                     /gene="alaE"
                     /locus_tag="JNZ05_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HAY8566872.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKGWIKNLADILAYVTFQSPVYVAILLVVGAD
                     WHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(4593..4943)
                     /locus_tag="JNZ05_003850"
     CDS             complement(4593..4943)
                     /locus_tag="JNZ05_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HAY8566873.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERQRHEHYGIPHGFSSRV
                     ALERYLNGLFGEAS"
BASE COUNT         1259 a         1169 c         1166 g         1356 t
ORIGIN      
        1 tgccggggct gaaagccgtt ggatcttatc gctcggttct ggaattgctc aatattcccc
       61 atgcgaaatt aatcatggac tgtgtgatat tactttccgt aaccagttgc ctgaactcgg
      121 ccctgtatac cgcctcaagg atgctctact ccttaagccg tcgcggtgat gctcccgcgg
      181 taatgggtaa aatcaaccgc agtaaaaccc cgtatgtggc ggtgttactc tccaccggcg
      241 cagcattctt aacggtggtg gtgaactatt acgcgcctgc gaaggtattt aaatttctga
      301 tcgacagctc cggcgctatc gccctgctgg tttatttagt catcgccgtt tcacagttgc
      361 ggatgcgcaa aattctgcga gcagaaggaa gcgaaattcg cttgcgaatg tggctttatc
      421 cctggctcac ctagctggtc atcggcttta ttacctttgt gttggtagtg atgctattcc
      481 gtccggcgca acagttagaa gtgatctcca ccggcttatt agcgataggg attatctgta
      541 ccgtgccaat tatggctcgc tggaaaaagc tggtattgtg gcaaaaaaca cccgttcata
      601 atacgcgctg ataatgatca ggagtcacac catgaccatt acgtctctgg atggctatcg
      661 ctggctgaag aacgatatta ttcgcggtaa ttttcaaccg gatgaaaaat tacggatgag
      721 tttactgaca tcgcgttatg cacttggcgt tgggccgttg cgggaagtac tttctcaact
      781 ggtggcggaa cggctggtca cggtggtgaa tcaaaaaggg tatcgggtgg cgtctatgtc
      841 agagcaggag ctgctctgac atagacgccc gcgccaatat ggaagcgatg ctggtcagtc
      901 tggcgattgc ccgcggtggc gatgaatggg aggcagacgt tctcgcaaaa gcgcatctgc
      961 tcaataagct ggaagcctgt gacgccagcg agaaaatgct tgatgagtgg gatctgcgtc
     1021 atcaggcgtt tcatacagca attgtggcgg gctgtggttc tcactatttg ctgcaaatgc
     1081 gtgaacggtt gtttgatctg gcggcgcgtt atcgatttat ctggctgcgg cgaacggtgc
     1141 tttcggtgga aatgctggag gataaacacg atcagcacca gaccctgact gcggcggtac
     1201 tggcgcgaga tacagcgcgc gccagtgagt taatgcgcca gcatttactg acgccaattc
     1261 ccattatcca gcaggcgatg gctggcaatt aactactctt ccggaatacg caacacctgc
     1321 cccggataaa ttttatccgg gctttttagc atcggtttat tcgcttcgaa gattttattg
     1381 tacagattag cgttaccgta gacctgtttg gaaatggcac tcagagtgtc gccagactta
     1441 acggtataaa actggctggc agtggctggt gtcgccgttt tcacctgatc atcaacactg
     1501 gcaataccgg aaatattccc caccgcaaca aggattttct ccttcgcctc ctgactcagg
     1561 ccgtcaccag tgaccgtcgc tttgccgtcg gcaatttgaa tgttcacttt atcggcatca
     1621 ggtataccgg ttttgctcag atgctcctgc accttcttcg cctgatcgtc tttatcgtgc
     1681 tgacctgtaa ccgcatccca gagtttttct ccggcatctt tcacaaaatt gaacagaccc
     1741 atagctacct cattcgttaa cggaaacact cagaaagtgt agcagaggaa cgcggcgcgt
     1801 taacgccgcg ggggtgagca caggtttaat cgcgggtttg cacccagaac gcgtgaatca
     1861 aaccggggat atacccaagc agtgtcagca gaatattaat aatgaacgcc caaccgaacc
     1921 ctttaccgag cagcacgccg agcggcggca gaatgatggt gatgacgatt ctccagaaac
     1981 ccatatgtac tccctataag aaaattactc attgtttaaa aagagacttt atctcttaag
     2041 cgtagtaatt ttagcggagc ctgccagttt cagcttacgc ttttactctc atttacgctg
     2101 ctgaggctgg tatatgttgc atttttagtt tagagtttac ttaatttaga aaatacttaa
     2161 ataaatatga ctgaactcgc gcaattacag gccagtaccg aacaggcagc ggccttattg
     2221 aaagcaatga gccaccctaa acggttgctg attctgtgca tgcttagcgg ttcccccggc
     2281 accagcgcgg gagagctgac gcgcattacc ggactgagcg cctctgcgac atcacagcat
     2341 ctcgctcgta tgcgggacga agggcttatc aacagccaac gggatgccca acgcattcta
     2401 tattccatta aaaatgaggc ggtaaatgcc attatcgcca ccctgaaaaa tgtctattgt
     2461 ccgtaaggag tcactatggc tttgacaacc atttcgccgc atgatgcaca agaattaatc
     2521 gcacgcggcg caaagttaat cgatattcgt gacgctgatg aatatcttcg tgaacatatt
     2581 cctgaagcag atctggctcc attatccgtg ctggaacagt caggtcttcc acctaaatta
     2641 cgtcgcgagc aaattatatt ccactgccag gcaggtaaac gcaccagtaa taacgccgat
     2701 aaattagcag cgattgccgc ccccgcagaa atatttttac tcgaagatgg gattgatggc
     2761 tggaaaagag ctggattgcc agtagcggta aataaatctc aacccttgcc gttaatgcgc
     2821 caggtgcaga tcgctgcggg tggtttaata ttaatcggcg ttgtactggg ttataccgta
     2881 aatagcggtt tcttcttatt aagtggcttt gttggtgccg ggttactgtt tgcaggaatc
     2941 agcggttttt gtggaatggc aaggttgtta gataagatac cgtggaacca acgagcttga
     3001 gaagcgacgc cggacgcgcc ctagcagcga catccggcct cagtaattag atcaggaaat
     3061 cgtcgagaga tttaccttct gccagcgcct gagcaattgg cttcggtgta cggccctgac
     3121 cggtccaggt tttagtttca ccgttaacat cagtgaattt atatttcgcc ggacgcggct
     3181 ggcgtttttt accagcacgt ggtgcagcag cagagctatt acccaataac tcttccgggt
     3241 taattccgtc agctttcatc agctccagcc aggtgctaat tttttcctga cgctcagcca
     3301 gttcacgctg ctgctgttct tcttcttcac gtctttcttt agtgacaacc ctgaattttt
     3361 cgagcatttc ttcaagaacg tcaatggaga attcgcgagc catcgcacgg agggtgcgaa
     3421 tgttatttaa actttgtaac attacggaca taataaagaa aaccttttaa cgccaaaaca
     3481 aaaaagtatt tcgtcgcgta gtgtaagcga tcacttatta tatttccaca aaatttaatc
     3541 tatttacagt cctgcgcgag attatttcag ctgttatttt ctgaaactct cagaataatt
     3601 tcaagattat atattagcca ttttgatgag taatttaggt tattggtgaa cagagtggcg
     3661 ctctctgctc gctaactccg taagcctgac aaaaccgaag tgaataaaaa tgcaatataa
     3721 cagattaaaa gaaaacaccc ctcactggtg ctttctgctg ttaatgtaca ttttctggca
     3781 tcaaaaatgc tgtaaatgac ccggtcacga caaacaccga attctctacg cttatagcgt
     3841 tacctccccc ccaaactttc attagtgaaa ttaaaggcaa ggccatatcc aattgattta
     3901 attgaaaata aatcatatcg acatgtaaaa atccacagtg tacaaaaaac gagccgttac
     3961 ggaatatttt atctacaaaa actgactaaa taaaaatttt tcactaattg attagtcata
     4021 gccagcgata tacgctatgc gaaaatacag atggcaatga gatccactgc tttcatctcc
     4081 attaacatcc cattacgctt ttattaagga gcattagcat gttctcaccg cagtcacgct
     4141 tgcgtcatgc agttgcagat acgttcgcga tggttgttta ctgttctgtc gtgaacatgt
     4201 gtattgaagt tttcctctcc ggaatgagct tcgaacagtc tttttattcc agattggtag
     4261 cgattccggt gaacatctta attgcatggc catacggtat gtaccgcgat ctgtttatgc
     4321 gcgcggcacg caaaggctgg ataaaaaatc tggctgatat cctggcttat gtgacgttcc
     4381 agtcaccggt gtatgtggcg atcttgttag tggtgggcgc agactggcat cagattatgg
     4441 cggcggtcag ttcaaacatc gttgtttcga tgttgatggg ggcggtttat ggctacttcc
     4501 tcgattattg ccgccgactg tttaaagtca gccgttacca gcaggtaaaa gcctgactct
     4561 tcttttcgcg actggcatcg ccagtcgcgt ctttaactgg cttcgccaaa taaaccattc
     4621 aaataacgtt caagcgcaac acgcgaacta aagccatgcg ggataccgta atgctcgtgt
     4681 cgctgtcgct cgcctgctaa atagagcgga aattgctggt aatgatcgca agttttaata
     4741 tcggaagcgg gttcagccag tgttgaactg cgttctttta atgttggatg aattaccaga
     4801 gcggtacgtc ccattcgcgc ttcgcgatta acatagacat aattttcccc acggcggtaa
     4861 ccatacgctt tctgggttac cacatcgaca gtaaaaccga ctttttcaag tacgcgcgcc
     4921 acctcgtctg gtcgtaaata catatttgat
//
LOCUS       DADMEQ010000194         4846 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.196, whole genome shotgun sequence.
ACCESSION   DADMEQ010000194 DADMEQ010000000
VERSION     DADMEQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.196"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            94..429
                     /locus_tag="JNZ05_003851"
     CDS             94..429
                     /locus_tag="JNZ05_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566874.1"
                     /translation="MARVEGELFKIARASLEAEPIAWRYRYVKKGVMDSQGELWVGDW
                     KYVPKKEDCNDRPNYEIQALFTAPPVPVTSEELVKAVHFYEQLKRENPPASGNQINGL
                     TMSVKRPAN"
     gene            603..821
                     /locus_tag="JNZ05_003852"
     CDS             603..821
                     /locus_tag="JNZ05_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566875.1"
                     /translation="MFKCYCTLIVGGIMKNALQFLFVAFWLFASCMPIIFTARYMEKI
                     DVLILMFGHINALFLGVFMAVMCIEYWR"
     gene            1024..1236
                     /locus_tag="JNZ05_003853"
     CDS             1024..1236
                     /locus_tag="JNZ05_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY8566876.1"
                     /translation="MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRTGQTEVAVFTAYEPEE"
     gene            complement(1278..1463)
                     /locus_tag="JNZ05_003854"
     CDS             complement(1278..1463)
                     /locus_tag="JNZ05_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566877.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            1404..1682
                     /locus_tag="JNZ05_003855"
     CDS             1404..1682
                     /locus_tag="JNZ05_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566878.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            1756..2742
                     /locus_tag="JNZ05_003856"
     CDS             1756..2742
                     /locus_tag="JNZ05_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY8566879.1"
                     /translation="MPVFHNTRVLVEPEPKSMRNLPSGVVPAVRQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHMKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSMYRESDIVPGEQTSTSILKQRTKNLAPLPHAHQQQNPPQEETVVS
                     IAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTK
                     ADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            2743..3108
                     /locus_tag="JNZ05_003857"
     CDS             2743..3108
                     /locus_tag="JNZ05_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY8566880.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            3105..3815
                     /locus_tag="JNZ05_003858"
     CDS             3105..3815
                     /locus_tag="JNZ05_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HAY8566881.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQVGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFV
                     MRTRSKKRRHFQSKVLQK"
     gene            4014..4089
                     /locus_tag="JNZ05_003859"
     tRNA            4014..4089
                     /locus_tag="JNZ05_003859"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4047..4049,aa:Ile,seq:cat)
     gene            4091..4167
                     /locus_tag="JNZ05_003860"
     tRNA            4091..4167
                     /locus_tag="JNZ05_003860"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4125..4127,aa:Lys,seq:ttt)
     gene            4170..4245
                     /locus_tag="JNZ05_003861"
     tRNA            4170..4245
                     /locus_tag="JNZ05_003861"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4203..4205,aa:Thr,seq:tgt)
     gene            4249..4323
                     /locus_tag="JNZ05_003862"
     tRNA            4249..4323
                     /locus_tag="JNZ05_003862"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4282..4284,aa:Gly,seq:tcc)
     gene            4616..4831
                     /locus_tag="JNZ05_003863"
     CDS             4616..4831
                     /locus_tag="JNZ05_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY8566882.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT         1286 a         1076 c         1284 g         1200 t
ORIGIN      
        1 gcggtaatga aaatgactga attaacaaaa gaacaattaa tcgaagaagc caaattaaaa
       61 atagcgatta cgaaatgcca ccccaattca gggatggcgc gagtagaggg cgagttattc
      121 aaaattgcac gggcatcgct ggaagcagag ccgatagcgt ggcgatatcg ctacgtgaaa
      181 aaaggtgtta tggactctca gggggagttg tgggttggtg actggaaata tgtaccgaaa
      241 aaagaggatt gtaacgacag gccgaactat gaaattcagg ccttattcac tgccccacca
      301 gtcccggtta catcagaaga actggttaaa gctgtgcact tttatgaaca actaaaacgc
      361 gaaaatccac cagcatccgg caaccagatt aatgggttaa ctatgtcggt taaacgacca
      421 gccaactgaa aaagcggaaa cctgattata ggttgccaga taaggcaata agctacctgg
      481 cgcggaacgg actgataagt atggggaatg ttttacgatg agtatttaga ctaaagagtt
      541 tgtaacgcta tgtaagtgat tttttctggt ttagatattt atatgtccgg ccaaattgag
      601 gtgtgtttaa atgttattgc acattgattg tagggggaat aatgaaaaac gcattgcagt
      661 ttttgtttgt tgcgttctgg ttgttcgcat catgtatgcc catcatcttc acagcaaggt
      721 atatggaaaa aattgatgtt ttgatattaa tgtttggaca tataaatgcc ctttttttag
      781 gggtgttcat ggcggtcatg tgcattgaat actggcggta aatacagcga acgctattgg
      841 tttagttgga tatttactgt gccggacaaa aacggtttgc agggaaatct tagttaagta
      901 gaatgactgc gggtgcttga ggctatctgt ctcaggcatg aacaccaaaa ggcagataga
      961 gaaaagcccc agttaacatt acgcgtcctg caagacgctt aacattaatc tgaggctcaa
     1021 tccatgctga acacatgtag gttagcctct tacgtgccga aaggcaagga gaagcaggct
     1081 atgaagcagc aaaaggcgat gttaatcgcc cttatcgtca tctgtttaac cgtcatagtg
     1141 acggcactgg taacgaggaa agacctctgc gaggtacgaa tccgaaccgg ccagacggag
     1201 gtcgctgtct tcacagctta cgaacctgag gagtaagaga cccggcgggg gagaaatccc
     1261 tcgccaccgc tgatgtgtca ggcatcctca acgcacccgc acttaacccg cttcggcggg
     1321 ttttgttttt tctggtcgtt ctggtttaca atccatccgt cagcctgaac aactggcacc
     1381 tgctgcgcca gcagagaaaa cagatggcgc acgataccaa attttacaat tcggataact
     1441 ctgccgcccc tgccagcagg cacgggcggc gttctcatgc attcaaatct gactggtatc
     1501 agcacgaccc ctgcaccgaa gaacaggctg aatggctgat tcagcgctac cgcagacacg
     1561 gatacgagat taagaaagcc ctcagtctcg attatcgtca ctggataatc ttcgtcaggc
     1621 tcccttattc cgaacggcca ccgcgtccgt cctgcacatt ccagcaacgg atctggaggt
     1681 aatgtgcggg tattacttcg acctgttctg gttccggaac tcgggctggt ggtccttaag
     1741 ccaggccgtg aatccatgcc ggtattccac aatacccggg tactggtgga gccggaaccg
     1801 aaaagcatgc gtaatctgcc gtccggggtc gttcctgccg ttcgccagcc gctggtggaa
     1861 gacaaaacat tgctgccgtt tttcagtaac gcacgggtga ttcgtgctgc tggtggtgct
     1921 ggcgcattgt ctgactggct gttgcgccat atgaaatcct gccagtggcc acacggcgat
     1981 tatcatcaca gcgaaaccgt cattcaccgt tatggtaccg gcgcaatggt gttgtgctgg
     2041 cactgcgaca accagttgcg tgaccagaca tccgaatcac tcgagcaact tgctcatcaa
     2101 aacctgtcag catggatgat tgacgtcatc ggtcacgcaa taagcggtac gcaggagcgt
     2161 gaattatctc tggctgaatt atcctggtgg gcggtccgca atcaggtggc ggacgcgcta
     2221 ccggaagcgg tattacgtcg ttcgctgggg ttgcgtgcgg aaaaaatccg ctcaatgtac
     2281 cgtgaaagcg acatcgtgcc gggagagcag acctccacca gcatactgaa gcagcgcaca
     2341 aaaaatcttg cgccgctgcc tcacgcccac cagcaacaga atccaccaca ggaagagacg
     2401 gtggtcagca ttgccgttga tccggaatct ccggctcagt atctccagcg ccagaaacca
     2461 caacgggaag agatgcctgt atacacgcgc tgggtaaaaa cgcagaaatg catgacgtgt
     2521 ggcaatcagg cagatgatcc gcaccacatc attggtcatg gactgggagg gatgggaaca
     2581 aaggctgacg atttgtttgt tattccgctg tgtcgtaaat gccataacga actacacgcc
     2641 ggggtaaaag attttgaaga aaaacacggt agtcagttgt tgttgctgat tcgtttttta
     2701 atgcacgcga gaaattcggg tgttctgaag tggaaagcat aaatgaccga acgcatagaa
     2761 tttgttttgc cttacccgcc aacggtgaac acctactggc gacgtcgtgg cagcacatat
     2821 tttgtatcaa aagccggtga gcgttatcgc cgtgatgtgg cgctaattgt tcgccagcag
     2881 cgactgaaat taaacctgtc cggaaggctg gcgataaaga ttattgcaga gccaccggat
     2941 aagcgccgtc gtgacctgga caatatcctg aaggcaccgc tggatgcgct gacgcatgct
     3001 ggactactta tagacgacga gcagtttgat gaaatcaata ttgtgcgcgg tcagctcgtt
     3061 cctggtgggc ggctggggat aaaaatcaca gaactggggt gcgcatgaat aaccagtatt
     3121 tacagtttgt gcgtgagcag ctcattatcg ccaccgctga tttgagtggg gcaacaaaag
     3181 gtcagcttga agcctggcaa gagaatgcca tgttcgatac agggcgttac aggcgtaaaa
     3241 aaatccggta ccgcgatgaa gtgactggaa aaatgataac gcgggataat ccaccaatcc
     3301 cgggaaagca atcgctggcg aaggggacgt caattcctct ggtcagtccg gttgagtttt
     3361 cgacatcatc gtggcggcgg gctgttctgt ctcttgaaga acatcataaa gcctggttgt
     3421 tgtggtgtta cagcgggagt atttgttggg aatatcagat cgcgataaca cagtgggcgt
     3481 ggaatgaatt taatgctcaa tccggtacca gaaaaattgc agggaaaacg caggaacgcc
     3541 tgaaaaaatt aatctggctg gcggcgcagg cagtaaaagc agaacttttt ggtggggaag
     3601 gttatgaata ccaggttggt ggggaaggtt atgaatatca ggagctggca ttactggcgg
     3661 gagtaacaac caaaaactgg tccaaaacat ttactggtca ctgggttgta atgaaacaca
     3721 tttttcaccg actggatagt gaggctttat tgtttgtaat gagaacacgt tcaaaaaaaa
     3781 gacggcattt tcaaagcaaa gtgttgcaaa agtagattga aaggcatata ttcatgcaaa
     3841 tctgatattt tgccgatttt gtacgtgatg gcaaaagcaa acaaaacccg cccacaagcg
     3901 ggttttttgt gccacttatc tcggatagac atggtgaatg cgctggtgga ggagataagg
     3961 gtgatttttg aatgcttgca acattgattt cgtaacgtta ttatcctgcg ctcggccctt
     4021 tagctcagtg gtgagagcga gcgactcata atcgccaggt cgctggttca aatccagcaa
     4081 gggccaccaa ccgccactag ctcatcagga aagagcgtca accctttaag ttgagtgtgc
     4141 gaggttcgag tccccggtgg cggtccagtg ccgacttggc tcagtaggta gagcaactga
     4201 cttgtaatca gtaggtcacc agttcgattc cggtagtcgg caccatatgc gggcatcgta
     4261 taatggctat tacctcagcc ttccaagctg atgatgcggg ttcgattccc gctgcccgct
     4321 ccagttagag tctttcagtc tgcgatgatg ggaaatcccg gagtgactga aagacgttta
     4381 agttatgaat gatcgctttt ttttgcaaaa ttgctgtgca gaaatactaa ccttcgggca
     4441 ggcgatcatt cataagcact ctgcttttat tccgattaac tgtgagtggt ttgttggata
     4501 gagtgctttc cttactgtat atatcgtttc gcccgctttt gcggtttttt cttttcaaat
     4561 ccctttcatt tctcagtgta aaactacgcc atccgttatt tgcggaggtg aggctatgaa
     4621 atccatggac aaaatttcaa cgggcattgc ctacggcacc tccgcaggca gtgctggcta
     4681 ctggttttta cagtggcttg atcaggtcag tccgtcacag tgggctgcga ttggtgtact
     4741 ggggagtctg gttctgggct tcctgactta tctgacaaat ctgtacttca aaatcagaga
     4801 agacaagcgt aaggctgcga gaggtgaata atgtcgccat cattac
//
LOCUS       DADMEQ010000195         4811 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.197, whole genome shotgun sequence.
ACCESSION   DADMEQ010000195 DADMEQ010000000
VERSION     DADMEQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.197"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..213
                     /locus_tag="JNZ05_003864"
     CDS             <1..213
                     /locus_tag="JNZ05_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY8566883.1"
                     /translation="PQPSGCLLESQGSLCPICRQRITHETGWNVHHKVKKVMGGGEEL
                     SNLVLLHPNCHRQLHSCEAGSLKKGL"
     gene            356..577
                     /locus_tag="JNZ05_003865"
                     /pseudo
     CDS             356..577
                     /locus_tag="JNZ05_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(598..660)
                     /locus_tag="JNZ05_003866"
                     /pseudo
     CDS             complement(598..660)
                     /locus_tag="JNZ05_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1110..2522
                     /gene="ospC1"
                     /locus_tag="JNZ05_003867"
     CDS             1110..2522
                     /gene="ospC1"
                     /locus_tag="JNZ05_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HAY8566884.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHGSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2626..2775)
                     /locus_tag="JNZ05_003868"
                     /pseudo
     CDS             complement(2626..2775)
                     /locus_tag="JNZ05_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2851..4548
                     /gene="ospD3"
                     /locus_tag="JNZ05_003869"
     CDS             2851..4548
                     /gene="ospD3"
                     /locus_tag="JNZ05_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HAY8566885.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLNPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGFSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            4665..4784
                     /locus_tag="JNZ05_003870"
                     /pseudo
     CDS             4665..4784
                     /locus_tag="JNZ05_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
BASE COUNT         1656 a          876 c          906 g         1373 t
ORIGIN      
        1 ccgcagccgt caggctgcct actcgagtca caaggctctt tgtgtccgat atgccggcag
       61 cgcattaccc atgaaactgg ctggaacgta caccataagg tgaagaaagt tatggggggc
      121 ggtgaagaat tatctaacct tgtcttgtta catccaaatt gccatcgaca gttacacagt
      181 tgtgaagccg gctctctcaa aaagggctta taaaggcttg agcggtgtac cgggaaaccg
      241 gtatgcaccg ttcttagggg agggcagtcc agcaatgaac tgtccttacc cgacaaacat
      301 ccaaaacgaa cggaacgtgc tggaatccgc atataacccg ttgtaaagtg aggaaatgga
      361 cacctcactt gctcatgaga acgcccgcct gcgggcactg ttgcagacgc aacaggacac
      421 tatccaccag atggctgaat acaaccgcct gctctcacag cggatggcgg cttatgcttc
      481 cgaaatcaac cgactgaagg cgctggttgc gaaactgcag cgtatgcagt tcggtaaaag
      541 ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca ttttcacggg cgatctacgc
      601 cacacaggct ctgggttgca gtgactgctc cattaaggcg attttgaact cataagggaa
      661 gtaacaaaaa aactgcaaaa gtacaataat tatatatggc caaactaacc tctattaaaa
      721 aacatcagtt tttcacactt agttgagctt catgaaaaaa tcatcgagga tttcattgca
      781 gaatacacat tccactaact ggaagcactc cataagagga atcagagaaa gtatcgtttt
      841 tttatagtaa aattcattgc tgttcaataa aattgatata gttattaaat tatgaaaata
      901 atttcacaaa aaagattaac ttgagttctt aagcatcgga ttttagatat tttgaaatat
      961 ctggcatcac ataacatcaa aagtatataa tacccttatc taattacatg cagtatattg
     1021 tatatatact caaaaattgt aattcatacc ctgctatata ttgtagggaa agatattaat
     1081 taaaactgtt ttcatataag gttcatttta tgaatatatc agaaacactg aactcagcaa
     1141 atacccaatg caatatagat tctatggata acagattaca tacattgttt ccaaaagtga
     1201 catcagtgcg aaacgctgca caacaaacta tgccagatga aaaaaattta aaagatagtg
     1261 caaatattat taaagatttc tttaggaaaa ctatagcagc acagagttat agtagaatgt
     1321 tctctcaagg ctctaacttt aaatctttaa atatagcaat tgatgcacca tcagacgcta
     1381 aagcctcatt taaggctatt gagcaccttg acagattatc gaagcattat atatctgaaa
     1441 taagggaaaa acttcatcct ctttctgcag aggaactcaa tttgctttcg ctaattatta
     1501 attctgattt aatcttcaga catcaaagta attctgattt gtctgataaa attttaaaca
     1561 ttaagtcatt caataaaatt cagtctgaag gaatatgcac aaaacgaaac acatacgctg
     1621 atgatataaa aaaaatagct aatcatgact ttgtgttttt tggcgttgaa atctctaacc
     1681 atcagaaaaa acaccccctg aatacaaaac atcacactgt tgattttggt gcaaatgcgt
     1741 atatcattga tcatggctct ccatatggat atatgacatt aaccgatcac tttgataatg
     1801 ctattccacc tgttttttac catgagcacc aatcattttt agataaattt tcagaggtta
     1861 ataaagaagt tagtcgatac gtacatggaa gtaaaggaat tatagatgta ccaatattca
     1921 atactaaaga tatgaagtta gggctcggat tatacctgat tgactttatt agaaaaagtg
     1981 aagaccaaag cttcaaggag ttttgctatg gaaaaaatct tgcccctgtg gatctggata
     2041 gaatcataaa ctttgttttt cagccagagt accatatacc taggatggta agtacagaaa
     2101 acttcaaaaa agttaagatt agagaaatat ctttagagga ggctgttaca gcatctaatt
     2161 acgaagaaat taacaagcag gtcactaaca aaaaaattgc tctccaggct ctttttcttt
     2221 cgattactaa tcaaaaagag gatgtcgcct tatatatatt atctaatttt gagataacta
     2281 gacaagatgt tatttccata aagcatgagt tgtatgatat tgagtatcta cttagcgctc
     2341 ataattcaag ctgtaaagta cttgagtatt ttatcaataa gggattggtt gatgtaaaca
     2401 caaagttcaa aaaaactaat agtggggatt gtatgttgga taacgcaata aaatatgaga
     2461 atgcagaaat gataaaacta ttattgaaat atggtgcaac atctgacaat aaatatattt
     2521 aatcaaaatt gaatatcgtt tagcaaaagg cagtatttgc acaaaatatc gatatattgt
     2581 ttattgtcag tatggctcaa tgtgataata gttggaaagt ttgatgggtt tcgccccgtt
     2641 gtagcggtag tcgaactgga aggtcttcag gcactgctta cagcgataga gcagcccccc
     2701 tgaactggaa tggccgttcc ggataccccc ctgagtttca gagtaacggg gacaaaccac
     2761 atcaatcttt gccatcaatc atccaaaggg caaagagtac aacaacacta agtctgcgtc
     2821 acaacccatc aatgaaagga atatatacat atgccatcag taaatttaat cccatcaagg
     2881 aaaatatgtt tgcaaaatat gataaataaa gacaacgtct ctgttgagac aatccagtct
     2941 ctattgcact caaaacaatt gccatatttt tctgacaaga ggagtttttt attaaatcta
     3001 aattgccaag ttaccgatca ctctggaaga cttattgtct gtcgacattt agcttcctac
     3061 tggatagcac agtttaacaa aagtagtggt cacgtggatt atcatcactt tgcttttccg
     3121 gatgaaatta aaaattatgt ttcagtgagt gaagaagaaa aggctattaa tgtgcctgct
     3181 attatttatt ttgttgaaaa cggttcatgg ggagatatta ttttttatat tttcaatgaa
     3241 atgatttttc attccgaaaa aagcagagca ctagaaataa gtacatcaaa tcacaatatg
     3301 gcattaggct tgaagattaa agaaactaaa aatggggggg attttgtcat tcagctttat
     3361 gatcccaacc atacagcaac tcatttacga gcagagttta acaaatttaa cttagctaaa
     3421 ataaaaaaac tgactgtaga taattttctt gatgaaaaac atcagaaatg ttatggtctt
     3481 atatccgacg gtatgtctat atttgtggac agacatactc caacaagcat gtcctccata
     3541 atcagatggc ctaataattt acttaacccc aaagttattt atcacgcgat gcgtatggga
     3601 ttgactgagc taatccaaaa agtaacaaga gtcgtacaac tatctgacct ttcagacaat
     3661 acgttagaat tacttttggc agccaaaaat gacgatggtt tttcaggatt gcttttagct
     3721 ttacaaaatg ggcattcaga tacaatctta gcatacggag aactcttgga aacttctgga
     3781 cttaaccttg ataaaacggt agaactacta actgcggaag gaatgggagg acgaatatcg
     3841 ggtttatccc aagcacttca aaatgggcat gcagaaacta tcaaaacata cggaaggctt
     3901 ctcaagaaga gggcaataaa tatcgaatac aataagctga aaaatttgct gaccgcttat
     3961 tattatgatg aagtacacag acagataccc ggactaatgt ttgctcttca aaatggacat
     4021 gcagatgcta tacgcgcata cggtgagctc attcttagcc cccctctcct caactcagag
     4081 gatattgtaa atttgctggc ctcaaggaga tatgacaatg ttcccggact tctgttagca
     4141 ttgaataatg gacaggctga tgcaatctta gcttatggtg atatcttgaa tgaggcaaaa
     4201 cttaacttgg ataaaaaagc agagctgtta gaagcgaaag attctaatgg tttatctgga
     4261 ttgtttgtag ccttgcataa tggatgtgta gaaacaatta ttgcttatgg gaaaatactt
     4321 cacactgcag accttactcc acatcaggca tcaaaattac tggcagcaga aggcccaaat
     4381 ggggtatctg gattaattat agcttttcaa aataggaatt ttgaggcaat aaaaacttat
     4441 atgggaataa taaaaaatga aaatattaca cctgaagaaa tagcagaaca cttggacaaa
     4501 aaaaatggaa gtgattttct agaaattatg aagaatataa aaagctgaat attatctcgt
     4561 ggtgatacac tgaatttgag gtaaccggct catttaaacc gtctggtctg ttacctccgg
     4621 ttccacaaaa ataatgtcca tcattttgaa gtggacacta tcgtatggaa taccggacct
     4681 ggatcactga agctttacgc cttcatttcg aagaacattt accacgggtt gtggccggac
     4741 gtcgcttggg cgtaccaaaa tcaactgtct gtggtatgtt cgtgtgaacc gcccccggta
     4801 tcccgctcat g
//
LOCUS       DADMEQ010000196         4628 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.198, whole genome shotgun sequence.
ACCESSION   DADMEQ010000196 DADMEQ010000000
VERSION     DADMEQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.198"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..942
                     /locus_tag="JNZ05_003871"
     CDS             <1..942
                     /locus_tag="JNZ05_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY8566886.1"
                     /translation="LSNHNISPRFMTEWDIFADQTSMITAGYNRYYGGNILDMGLRDI
                     RNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARANYVYREAHDQISKS
                     SRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFSYIKSKGNLS
                     LNNGYEENNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPSGLVWANTLA
                     WQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQFLKQQNLTIS
                     ADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            987..3782
                     /locus_tag="JNZ05_003872"
     CDS             987..3782
                     /locus_tag="JNZ05_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY8566887.1"
                     /translation="MEIIMRNLCFLLTLVATLLLPGRLIAAALQQDEKLITGQLDNGL
                     RYMIYPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGAKTWPGNKVIETF
                     ESMGLRFGLDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAE
                     RGVISEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQR
                     WYQPNNMTFIVVGDIDSKEALALIKDNLSKFPANKAAENRVWPTKAENHLRFNIINDK
                     ENRVNGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTA
                     RSVKIAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWL
                     KNAVDQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQL
                     RKNQDAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQ
                     AEISSKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIA
                     LANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLIN
                     QRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIA
                     QFTAADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPL
                     TRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIRE
                     QASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQEL
                     NEYQQNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTIENVNTAVK
                     QYLSHPVNTYTGVLLPK"
     gene            4144..>4628
                     /locus_tag="JNZ05_003873"
     CDS             4144..>4628
                     /locus_tag="JNZ05_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY8566888.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PV"
BASE COUNT         1383 a         1042 c         1052 g         1151 t
ORIGIN      
        1 ctgtcaaacc acaatatctc cccgcgcttt atgacggaat gggatatttt tgctgatcaa
       61 acctcaatga ttaccgccgg ttataaccgt tactatggcg ggaatattct tgatatggga
      121 ttacgtgata tccgcaatag ctggacggaa tcggtatcag gtaataaaac cctgacgcgt
      181 tatcagaatt tgaaaacgcc ttataacgat gaactggcaa tgggattgca gcaaaaaatc
      241 gataagaacg ttattgcacg cgcaaactat gtttaccgtg aagcgcatga tcaaatcagc
      301 aaaagcagtc gtaccgacag cgcgactaaa accaccatta ctgaatataa caacgatggc
      361 aaaaccaaaa cgcattcgtt taacctcagt tttgaactgg ccgaacccct gcatatccgc
      421 caggtagata ttaacccgca aattgtcttt agctatatca agagcaaggg caacttgtcg
      481 ttaaacaatg gttatgagga gaacaatacc ggtgataacc aggtggttta taacggtaat
      541 ctggtctctt acgatagcgt tccagtggca gattttaata acccattaaa gatctcctta
      601 aacatggatt tcacgcatca accgagcggg ttagtgtggg cgaatacgct ggcctggcaa
      661 gaagcgcgta aagctcgcat tatccttggt aagacaaatg cgcaatacat cagcgaatat
      721 tcagattaca agcagtatgt tgacgaaaaa ctggatagca gcctgacctg ggacacccgc
      781 ttgtcctgga cgccacaatt tctgaaacaa caaaacctga cgatcagtgc cgatattctc
      841 aatgtactgg atagcaaaac cgctgttgat acaacgaaca ccggtgtggc gacctacgcc
      901 agtggccgta ctttctggct tgatgtcagc atgaaatttt aatagttcgg attttactta
      961 accgggtaaa ttcgcccggt tttcgcatgg agataattat gagaaacctc tgtttcttac
     1021 tgacgttagt ggcaactctg ttgctccccg ggcggctgat tgccgccgcc ttacagcagg
     1081 atgaaaagtt aattaccggg caactggaca atggcttgcg atatatgatt tatccgcatg
     1141 ctcatccaaa ggatcaggta aatttatggc tgcaaattca taccggttca ttgcaggaag
     1201 aagacaatga gcgcggcgtg gctcattttg tagaacatat gatgtttaac ggcgcaaaaa
     1261 catggccggg taataaagtc atcgaaacat ttgagtcaat gggcctgcgt tttggtctcg
     1321 atgttaatgc ctataccagc tatgacgaaa cggtgtatca ggtgagtttg ccgactacgc
     1381 agaaacaaaa tctgcaacaa gtgatggcaa tcttcagtga atggagtaat gccgcaacct
     1441 ttgaaaaact cgaagtagac gctgaacgtg gcgtaatttc tgaggaatgg cgtgcccatc
     1501 aggatgcgaa atggcgcacc tctcaggcgc gccgcccttt cctgctggct aatacccgta
     1561 atttagaccg tgaacctatc ggcctgatgg atactgtcgc cacggtcaca ccggcacaat
     1621 tgcgccaatt ttatcaacgc tggtatcaac caaataatat gacctttatc gtggtcggcg
     1681 atatcgacag taaagaagcg ctggcgctga taaaggataa tttaagtaag tttccggcta
     1741 acaaagcagc tgaaaatcgc gtctggccga caaaagccga aaaccacctg cgctttaata
     1801 tcattaatga taaagaaaac cgggtgaacg gcatcgcact ctattatcgc ctgccaatgg
     1861 tacaagtgaa cgatgagcaa agctttgtcg aacaagctga atggagcatg ttagttcagc
     1921 tgttcaatca acgtctgcag gaacgcatac agtcgggcga gttgaagact atttctggcg
     1981 gcactgcgcg cagcgttaaa attgcacccg attatcagtc gctgtttttc cgtgtaaatg
     2041 cacgagacga taatatgcag gatgctgcga atgcattaat ggcagagttg gcaaccattg
     2101 atcagcatgg tttttctgct gaagaactcg atgatgtcaa atctacccgc ctcacctggc
     2161 tgaaaaatgc ggttgatcag caagctgaac gtgatttacg tatgctgacc agtcgcctgg
     2221 catccagctc attaaataat acgccgttct tgtcgccgga agagacatat caactttcga
     2281 aacgtctgtg gcagcaaatt accgtgcaaa gtctggcgga aaaatggcag cagttaagaa
     2341 agaaccagga cgcattttgg gagcaaatgg taaacaatga gcttgccgcc aaaaaagcat
     2401 tgtctcctgc ggctatcctg gcgctggaaa aagagtacgc caacaaaaag ctggcggctt
     2461 acatcttccc aggcagaaat ttatcgttaa cagtagacgc tgacccacag gcggaaatta
     2521 gcagcaaaga aacgctggct gagaatctga catcattaac actttccaat ggtgccaagg
     2581 ttattctggc aaaatccgcg ggtgaagagc aaaagctaca aattattgcc gtatcgaata
     2641 aaggcgattt aagtttccct gcgcagcaaa aatcacttat cgcgctggca aataaagcag
     2701 ttagcggaag cggcgttggc gaactctcct cttccagcct gaaacgctgg agtgcggaaa
     2761 actcggtaac catgagcagt aaagtcagtg gcatgaatac gttgctctcc gttagcgcgc
     2821 ggactaataa ccctgaacct ggtttccagt tgattaacca gcgaatcacc cacagcacga
     2881 ttaacgacaa tatttgggca tcgctacaaa atgctcaaat tcaggcgttg aaaacgctcg
     2941 accagcgtcc agcggagaaa ttcgcccagc tgatgtatga gacgcgctat gctgatgacc
     3001 gcacgaaatt actgcaagaa aatcagattg cacagtttac tgccgcagat gcgctggctg
     3061 ccgatcgcca gttgttttca tctccagcgg atatcacgtt tgtcattgtc ggtaatgtcg
     3121 cagaagacaa actcgtggcg ttaattacgc gttacttagg ctcaatcaaa cactctgatt
     3181 cgccattagc cgcaggtaaa ccattaactc gcgcgacgga caacgcatcg gttactgtaa
     3241 aagaacaaaa tgaacctgtg gcacaggttt cacagtggaa gcgttatgat tcccggacac
     3301 ctgttaatct ggcgacgcgt atggcgctcg atgcttttaa cgtcgcactg gcaaaagatc
     3361 tacgcgttaa tattcgtgaa caggcatctg gggcatacag cgtttcttct cgcctctcgg
     3421 ttgatcctca ggctaaagat atcagtcatt tgctggcttt tacttgtcaa ccagaacgac
     3481 atgatgaaca gttaacgtta gcgaatgaag tgatggttaa gcgtttgact aaggggatca
     3541 gtgagcaaga actgaacgaa taccagcaaa acgttcagcg cagcctcgat atccaacagc
     3601 gtagcgttca acaattagcg aacactatta taaatagtct tattcaatat gacgatcctg
     3661 cagcatggac tgagcaggag caattgttga aacaaatgac gatagagaat gttaacactg
     3721 ccgttaaaca atatctttct catccggtaa atacttatac cggagtatta ttgccaaaat
     3781 aataacagcc ccgtcaacac atcgttggcg gggattttag caatattcgc tatttttatg
     3841 taataatttt ataaatgcgt tcaaaataat aaccaagtac taatagtgat attttaatgt
     3901 ctgattttta cgtgataatt caggaggcac agaaagcgca taaaaataac aacataaaac
     3961 accttgccac cacccaagaa ttctctattg cattgttttt aatgaaaaaa atatcattcc
     4021 aacaatatct cacgataaat aacattagga ttttgttatt taaacacgag tcctttgcac
     4081 ttgcttactt tatcgataaa tcctactttt ttaatgcgat ccaatcattt taaggagttt
     4141 aaaatggata agaagcaagt aacggattta aggtcggaac tactcgattc acgttttggt
     4201 gcgaagtcta tttccactat cgcagaatca aaacgttttc cgctgcacga aatgcgcgac
     4261 gatgtcgcat ttcagattat caatgatgaa ttatatcttg atggcaacgc tcgtcagaac
     4321 ctggccactt tctgccagac ctgggacgac gaaaacgtcc acaagttgat ggatttatcc
     4381 attaacaaaa actggatcga caaagaagaa tatccgcaat ccgcagccat cgacctgcgt
     4441 tgcgtaaaca tggttgccga tctgtggcat gcgcctgcgc cgaaaaatgg tcaggccgtt
     4501 ggcaccaaca ccattggttc ttccgaggcc tgtatgctcg gcgggatggc gatgaaatgg
     4561 cgttggcgca agcgtatgga agctgcaggc aaaccaacgg ataaaccaaa cctggtgtgc
     4621 ggtccggt
//
LOCUS       DADMEQ010000197         4524 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.199, whole genome shotgun sequence.
ACCESSION   DADMEQ010000197 DADMEQ010000000
VERSION     DADMEQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.199"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            134..577
                     /locus_tag="JNZ05_003874"
     CDS             134..577
                     /locus_tag="JNZ05_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8566889.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQAHLGHRSSSMTDKYRDGRGREWKDI"
     gene            813..1632
                     /locus_tag="JNZ05_003875"
                     /pseudo
     CDS             813..1632
                     /locus_tag="JNZ05_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(1633..2691)
                     /gene="modC"
                     /locus_tag="JNZ05_003876"
     CDS             complement(1633..2691)
                     /gene="modC"
                     /locus_tag="JNZ05_003876"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HAY8566890.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRLMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2694..3383)
                     /gene="modB"
                     /locus_tag="JNZ05_003877"
     CDS             complement(2694..3383)
                     /gene="modB"
                     /locus_tag="JNZ05_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HAY8566891.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3383..4156)
                     /gene="modA"
                     /locus_tag="JNZ05_003878"
     CDS             complement(3383..4156)
                     /gene="modA"
                     /locus_tag="JNZ05_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8566892.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKANEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4323..4472)
                     /locus_tag="JNZ05_003879"
     CDS             complement(4323..4472)
                     /locus_tag="JNZ05_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump-associated protein, AcrZ
                     family"
                     /protein_id="HAY8566893.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
BASE COUNT         1228 a         1182 c         1077 g         1037 t
ORIGIN      
        1 tcttgccgaa gaagatccag cttctgcaac caggccattc agaactgaag tgaagcgcag
       61 tcgactgtta attgacgagt atctgttaat caggaaaata gcagacgtcc agaacgaatg
      121 gttcggcctt tgcatggatc ttgcactggt aacaggacag cgcgaaggag atctggcggc
      181 tatgagatgg gaggatataa gagatagcag gctatacgtg gaacaacaaa aaacaggggc
      241 aaaaattcgc atttctctcc ccaccacgat ttccagattg aatttaacac ttgccgatgt
      301 actggataac ttgaaaaaaa taaacggtaa aaacgaaaag ttactcggag ggaagacagc
      361 caggacaatc gcagcacagt tcaggattgc ccgagacaca tccggattga aatgggaagg
      421 tgacccaccg ccatttcatg aaatccgaag tttatccggt cgcctgcact cagcagaaaa
      481 aggcagtgat ttcactcagg cgcaccttgg acatcgttca tccagcatga cggataaata
      541 ccgtgacgga cgagggaggg aatggaaaga catttagaat taatttcgaa tgatttcgaa
      601 atactacttg taatcaattg attataaagg attaattcct aaagagcatt tgcttcatga
      661 atgctccttt gcatttagca actgactgta acagctaaaa ttagtcgctt ttggcggtga
      721 atgctgaatt aaaacgcaga attttgtccg atagtggtat cggtgtacca ttcgcgcaga
      781 cgaaaattct taaccttaat ctggaatacg ccatgaccac acgcgtgatt gctctcgact
      841 tagacggcac cttattgacc ccgaaaaaga ccttgcttcc atcatcgata gaagccctgg
      901 cccgcgctcg cgaagcaggc tatcaattaa tcatcgtcac aggtcgccat cacgtcgcta
      961 ttcatccttt tttatcaggc gctggcgctg gatacacctg ctatttgctg taatggcacc
     1021 tatttgtatg attatcatgc aaaaaccgtg ctggaagcgg acccaatgcc cgttaataaa
     1081 gccctgcaac tcattgagat gctgaatgaa caccacattc acggtctgat gtatgtcgat
     1141 gatgcaatgg tctatgagca cccgaccggg catgtcattc gcacgtctaa ctgggcgcaa
     1201 accctgccgc cggaacagcg tccgactttc acacaagtcg cttctctggc tgaaacgacg
     1261 caacaagtta acgccgtatg gaagttcacc ctgacgcacg atgacctgcc gcaattgcag
     1321 cattttggta agcatgtcga acatgaactg ggactggagt gtgaatggtc ctggcacgat
     1381 caggttgata ttgcacacgg cggcaacagc aaaggtaaac gtttgacgaa atgggttgag
     1441 gcgcaaggtt ggtcgatgga aaacgtcgtg gcattcggcg ataactttaa tgatatcagt
     1501 atgctggaag ccgctggtac aggcgtggcg atgggcaacg ccgatgacgc ggtaaaagcg
     1561 cgcgccaaca ttgtgattgg tgataacacc accgacagca ttgcccagtt catttatagc
     1621 cacctgattt aatcaggcgg ttatcgacac acttttaatt tgcgcgtaca gccacaggcc
     1681 aggtttgatc gccagttcat ccctggccca cgggctgata cgcgcccaca gcgttttacc
     1741 gccgacttcc agctccactt ccacctggcc gttgtcgtca taactattaa caacttttgc
     1801 ccgcaatacg ttacgaatgc tggtttgctg cggcggttgt aaaaccagag aaacatcgga
     1861 agcctgaatg cggatacgta gcgcagcttg cagcggttcg cccagcttat tgacccacaa
     1921 atgctgatcg cccagcgcca gcgcggtcat cgcgtaatgc ggatgatgct caagcaccgt
     1981 cactttcaga atgctacttt gttgctcttt cggcagccac ggattcatca cgctactgcc
     2041 ccacacttcc tccagcgcgc caaaggcttt cacctgaccg ttttccagta ccatcaatct
     2101 gtctgccaga tggaggatct catccagcga atggctgaca tacaacatcg gaatgttgat
     2161 ttcccgtgtc agccgttgca gataaggcaa cagttcgcgt ttacgcggaa tatccagtga
     2221 cgccagcggt tcatccagca acagcaattc cggtgctgtc agcaaagccc gaccaatcgc
     2281 cacgcgctgt ttttcgcctc cggacaggct gcctggtaaa cggtcaagca acggttcaat
     2341 gcctaaaagc gccaccagct tatcgaactg atcgaccata cttttcgaca tgccgtagcg
     2401 cagattgcca cgcactttgt aatgcgggaa caaccgcgca tcctgaaaaa catagccaac
     2461 acgacgcttt tccggcgtca ggcagatacc tttttcggca tcatttagta cccgcccatt
     2521 gaggacaatc cgcccttttt gcgggcgcgt cagtccactg atggcgttaa tcagcgaagt
     2581 ttttccggca ccggagacgc caaagatagc agtgatgcca ttggcgggca gcgtttcatt
     2641 aatggtcagg caatggttgc ccaacgtctg ggaaaaattc agctccagca tgattagcgc
     2701 cccgcccgtt cacggctgat tctggccagc cattctgaaa tcaacaggga gatcatcgcc
     2761 agcgcaatag aaataatgca cagtctcgcc gcgccacttt ccccgccggg ggtctggatc
     2821 agggtataca tggcagaagg aatggttcgc gtttcgctag gaatgttcga cacaaaggtg
     2881 atggttgcac caaactcacc gagagaacga gcaaaagcca gtaccgtacc aacaataatt
     2941 cccggtaagg tcagcggtaa cgtgatagta aagaaaacgc gccagcgccc ggcacctagt
     3001 gttcttgcgg cctgttccag tttgacatca accccttcca gcgccagacg aattgcccgc
     3061 accatcagcg gaaacgacat gacggcggca gcgagtaccg cgccgcgcca gctaaaggcg
     3121 aaggtaatac caaaccagtc atacagacgt tcaccgataa atccgcgccg tcccatcgaa
     3181 actaataata agtaaccgac gaccacgggc ggtaacacca gcggtagatg cagtacgctg
     3241 tcgagcagag ctttgcccgg aaacgtgcaa cgcaccagta accaggcaaa aaagatccca
     3301 aacggcaggc taaacagcac agccagggaa gaaactttca ggcttaataa aactgcctgc
     3361 cattctggat cggtcagtat cattacttgg ttgtaaatcc gtaacgttta aagatttccg
     3421 ctgcctgcgg tcccttcaga taatcataaa atgctttcac tgtcgcattg ttatgccctt
     3481 ccacaacagc aaccggatat tccacttttt tatgtgaatc ttccgggaag gtggcaacca
     3541 cttttacccc tttgctggca actgcgtcag aaccgtagac aatgcccaga ggcgcttcgt
     3601 tacgttcgac cagcgccagc gccccacgaa catcttccgc tggggccagt ttcggagaga
     3661 gcgtatccca tgcgcccagt ttttgcagtg cttcttttgc ataaatgcca gcgggaacat
     3721 gttccggatc gccaaccgcc aggcgaccgc cattcagcag cgaagtccag ttagttttgc
     3781 tgtcgatggt gaaatctttc tgctcgttgg ctttcggtgc tacaacgacc aggctattgc
     3841 cgagcagtgt ctgacgcgta gcagtatcga tcgctttttt atcaaccgca taatccatcc
     3901 atttctgatc ggcagaaata aacagatccg caggggcacc cgcttcaatc tgacgggcga
     3961 gagtagatga cgaagcgaaa gaagaaacca catccacgcc tttctctttt ttatactgcg
     4021 tagcaatgtc ctgcattgcg ttagtcagtg atgctgcggc gaacaccgtg attttccctt
     4081 catctgccag tgcattacca gcaactgcga aagagagtgc cgccccggca aacaagttca
     4141 accatttacg agccattggt aaccccttaa tgtaacgtta tgtaggagac aatataacga
     4201 ctaactcgga attttcccag ctgttattgc taacctttgt gaggtagata agaaaaaata
     4261 tcggcaggaa aagcaggaag ttgagaaaaa gaaaatgccc gactaagcgg gcattcaggg
     4321 aatcaatgat tttgtccggg ctggtctttt ttaccaacac cagaaaagat gttgaatact
     4381 tcaccaagac cgtaaatcag acccaggatg atggccatca cgacaggtac catgattacg
     4441 gcgaatacca gactttttaa taactctaac atggtcaact ccagatatag tcatgagact
     4501 attctaaccg ctaagcacag aaaa
//
LOCUS       DADMEQ010000198         4508 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.200, whole genome shotgun sequence.
ACCESSION   DADMEQ010000198 DADMEQ010000000
VERSION     DADMEQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.200"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            368..2020
                     /locus_tag="JNZ05_003880"
     CDS             368..2020
                     /locus_tag="JNZ05_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY8566894.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2031..2201)
                     /locus_tag="JNZ05_003881"
                     /pseudo
     CDS             complement(2031..2201)
                     /locus_tag="JNZ05_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2280..2393)
                     /locus_tag="JNZ05_003882"
                     /pseudo
     CDS             complement(2280..2393)
                     /locus_tag="JNZ05_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004989794.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2527..2853)
                     /locus_tag="JNZ05_003883"
     CDS             complement(2527..2853)
                     /locus_tag="JNZ05_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY8566895.1"
                     /translation="MPGASNAAMCKITETIKKWRIHRSTAESLLDFARRYNAIVRGWI
                     EYYGKFWSRNFSYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLALVRKQYPKLFAHWYL
                     LRASNE"
     gene            complement(3138..3790)
                     /locus_tag="JNZ05_003884"
                     /pseudo
     CDS             complement(3138..3790)
                     /locus_tag="JNZ05_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904490.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
BASE COUNT         1198 a         1090 c         1038 g         1182 t
ORIGIN      
        1 atcggaaacg atgatctggc agcctgagtt cacagataaa acattctcca ggaaacttgg
       61 ggcggttcac gtcagtatca gaccatatta attgctgaaa ctgataaaca tcaaacctta
      121 tagaagggtg aatgctgaaa gggggctatg gtgatcgacg tgatgacaac gacaggtaag
      181 accgtgacct gataaacctg aattgtgtcc tagacataaa gtccgtgaag ggaataaggc
      241 tcaggtcagc gaatcttata gtggcccgca gctctgactg caataaggcc ggatacagag
      301 cagcaactac tcgtcgatgt cagaacaacg gaagccttgc aaacggggat gcgttcatac
      361 aggtacaatg ataccgttaa aaaaaaacat tacgctaata atgttcaccc tgtcattact
      421 gacaggaaat cctgctatag cgtatgaaac agataaagta tataaaataa ccgttctgca
      481 tacaaatgac caccatggac acttttggcg taacaatcat ggtgagtacg ggttatcctc
      541 ccaaaaaact ctggtggaca atatccggca gaaagtaata aataatggcg gaagtgttct
      601 attattatct ggtggtgata ttaacaccgg tgtacctgaa tcagaccttc agaaggctga
      661 acccgatatt cgtggaatga atctaattgg ttatgatgca atggccgtcg gtaatcatga
      721 atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat ttcctttcct
      781 ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat ggaaaatttt
      841 catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata ctgcgaaaac
      901 aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg aggcgaggag
      961 tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg caacacatat
     1021 gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg aaatggctcg
     1081 cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag ctcctgtctg
     1141 catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct gtgtaccaga
     1201 taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg tcggtcaggc
     1261 tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac acccagttaa
     1321 tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt acacccccga
     1381 aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata aaggcaaagc
     1441 acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca gaagtaaagt
     1501 tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag aacgtattga
     1561 cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga gtggtaacat
     1621 cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa gtattgattt
     1681 gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac cggattccgg
     1741 agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa tcgaaatgct
     1801 aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa catttagctt
     1861 taatgctact ggtggggatg gctatccacg tattgataat agacccggat acataaatac
     1921 cggatttatt gatgctgaag tacttatcga atatatccgg aaacattcac cgctggatgc
     1981 tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt gacgatcatc
     2041 acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg gatgtttgcg
     2101 atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt gctgaattta
     2161 accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt cgctgattaa
     2221 agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccatat tgatatttac
     2281 taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc cggtcagtcc
     2341 tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt cagacggcat
     2401 gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctatcgga accccgccgg
     2461 agagagtttc actctccggc tgctcacgga accgtacgtg acactctcgc gtcatacggc
     2521 tcttgatcac tcattcgatg cacgtagtaa ataccagtgg gcaaatagct tcggatactg
     2581 tttccttacc agcgccagct ttcgctgagc cttccggttc gaaagtctgt atttagactg
     2641 catccactta agcaaacgtg actgcattgc actccacagt cgatagctga agtttctgga
     2701 ccagaacttt ccatagtact caatccagcc tctcactatc gcattgtagc gcctcgcaaa
     2761 atccagcaaa ctttcagctg ttgagcgatg tatacgccac ttcttgattg tttctgttat
     2821 tttgcacatt gctgcatttg atgcacccgg catacatttt ctgtacaatt cgcctttgaa
     2881 attcttcaga gtacgcactt tgaagtcata tccgagaaag gagaaactcg tagcgacgtt
     2941 tctcctttta aacgtgtcaa tgtaggcgat attcgtcttc cctgcgttca ggaccaaccc
     3001 gacttccgag aagcgcaccg ataacctgtt cttcagcctt gttgcatcac tcattcgtga
     3061 acaatgtact acaagtcgag tcacccgagg gaacctcccc ctctggtgct cacggaaccg
     3121 gacgtgaacc tctcagctca tccggctctt ctcattgagg cctgtcccca tagtaacggc
     3181 cagtgtgcaa ataacctcgt ttcactccgc gcaacacggg ccagccagtg gctggcccgt
     3241 cgtcggtggc ccctaagtcg cttgtgttta cggggcagcc ataacacgag tttgcgatcg
     3301 atttgcctca gcgtcagata cagtgctgat ttataaaaag caccgtagta ctttatccag
     3361 cctctgattt tcgcattgaa catatgcgcc agctcgtaca aggacttgtc actgcgattc
     3421 tcctcacctc ctgacggatt gcttttcctg ctctggcact catcgccgga ctgaagttaa
     3481 cgaaggtctt accccagcga ttcatcgaca gacgcggccg gaatgtatag cccagaaagt
     3541 tgaagctcgt ttccgggtag tccccccgtc ggtcagcatc cttacagtaa actattcgtg
     3601 ttttttgcgg atgcagctgc agcccgcatt gcgcaagccg ctgcctcagt ccacttatca
     3661 gagcgtcagc ctgccactgg ctgtgacagt ggcatacaac atcatcggcg tacctttcga
     3721 agggaacgcc gggaaactga cgttgcatcc acatatcaaa ggcatagtgc aggaacagat
     3781 ttgagagcag gggggagatt acgccgccct gcggcgttcc tcgaccccgt gccagcattg
     3841 ctccatccgg catttgcacc ggtgccttca gcctgcgctc aatgtacatc accacccagc
     3901 gtgcctgagt atgatgctgc actgtcttca gtagcaggtc atgatcgata tcatcaaaga
     3961 agcctttaat atcgacttcc accacccagt caaacttcca gcagcgtttc ctcgcctgcg
     4021 caatcgcctg gtgtgaggat ttacccggtc gataaccata cgagtctgca tggaacaggg
     4081 gttccagtac cggctcgagt atctgcttaa cgaccatctg ggcgatccgg tccgacacgg
     4141 ccggaatacc gagcggacgt acaccaccgt cagctttggg aatgtccaca cgccttaccg
     4201 cctgcggcat ataactgccg gaagccattc tgttccacag tttgtagaga ttgtctgtaa
     4261 cattctcgtc aaacccggcc agtgtctgcc cgtcaatgcc tgccgcccca cggttggctt
     4321 ttactcgttt ataagcttcc catacctgtc gtttacttac aggaaatggc tttgccgatt
     4381 ttacagcagt cctcccatcg gcattgtcgc ccgtggttgc cgccgcacca tcagcacaat
     4441 ggggcaagcc ctttgcttcg gacccattac ggtccttcac cgctactacg gtttactccg
     4501 tccctgta
//
LOCUS       DADMEQ010000199         4439 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.201, whole genome shotgun sequence.
ACCESSION   DADMEQ010000199 DADMEQ010000000
VERSION     DADMEQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.201"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..213)
                     /locus_tag="JNZ05_003885"
     CDS             complement(<1..213)
                     /locus_tag="JNZ05_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HAY8566896.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="JNZ05_003886"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="JNZ05_003886"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAY8566897.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLGGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="JNZ05_003887"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="JNZ05_003887"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HAY8566898.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="JNZ05_003888"
     CDS             2894..3634
                     /locus_tag="JNZ05_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY8566899.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="JNZ05_003889"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="JNZ05_003889"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAY8566900.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4192..>4439)
                     /locus_tag="JNZ05_003890"
     CDS             complement(4192..>4439)
                     /locus_tag="JNZ05_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566901.1"
                     /translation="KFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFLGSI
                     PWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1099 a         1106 c         1233 g         1001 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcag cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctgggagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagac atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggtcaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtctt acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctgg cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tctggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttg
//
LOCUS       DADMEQ010000200         4378 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.202, whole genome shotgun sequence.
ACCESSION   DADMEQ010000200 DADMEQ010000000
VERSION     DADMEQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.202"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(64..822)
                     /locus_tag="JNZ05_003891"
     CDS             complement(64..822)
                     /locus_tag="JNZ05_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAY8566902.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQYFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="JNZ05_003892"
     CDS             complement(830..1933)
                     /locus_tag="JNZ05_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY8566903.1"
                     /translation="MTKVFLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLILASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..3113)
                     /locus_tag="JNZ05_003893"
                     /pseudo
     CDS             complement(1943..3113)
                     /locus_tag="JNZ05_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3181..4206)
                     /locus_tag="JNZ05_003894"
     CDS             complement(3181..4206)
                     /locus_tag="JNZ05_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8566904.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1215 a         1146 c         1022 g          995 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatactg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 gaattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagcccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagga
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aacgcaataa
     2581 atccgtctcc cagctgcggt gacggaatat aaagcccacg attgctcaaa aaggccagat
     2641 caaacgcgct cacagcttgg cgtggtccgg gcaaattgcg caacacggca aagtaccaga
     2701 agaagatttg caatattacg gaagatctca atataaattg tggaaagctt tcgtagcagc
     2761 cagttatccg aaagtctcgc cagaccgata aagaagccca aaacagaagc gaacacaata
     2821 cacaatgcag aaaccagtag cgtattgagt aagccgacaa taaaaacgcg tccgtaggtg
     2881 tcgccctgct ggtaatcgat caaatgctgg acaataccga agccagcgcc gcgatccaga
     2941 aaggcaaaac ctgaagtaat gccacgatta ttgagattcg ttacagtgtt ttgaaataac
     3001 caaccaacaa tgccgacaac agcaacaacg gcaaggatct gaaataacca ggcacgaacc
     3061 gtagggttgg caaaggagag tgagccttta acggttgagc ggcgatgaga catagaaacc
     3121 tcggaaagca taacgctgaa gagagcgccg ccgtagcgac gctcgttacc tcacaccgac
     3181 ttaacgcacg ggcggcgcgt actgaatacc gccgttattc cagagattat tttgcccacg
     3241 tttaattttc agcgggcttt ctgaacctac gttacgctca aaaatttccg agtagttacc
     3301 cacctgtttg atgatgttat aggcccattt attatccaac ttcagatcct tgccgtaatc
     3361 gccttcttta cccaacagat gtgccatatc aggcgttgct ggattagccg ctttttcatc
     3421 gacgttctgg gaattgatgc ccatctcttc agcatttagc atggcgaaaa gcgtccagcg
     3481 tacaatcgag aaccattcat catcgccacg acgaactacc ggcccaagcg gttctttaga
     3541 gataacctcc ggtaagacaa tccattcagc cgggttgctt aatttgatgc gcaaggcata
     3601 cagttgcgac tgatccgaag ccagcgtatc gcagcgacca gattccagtg ccttcgctga
     3661 ttcgtcagag cgatcgaaag tcactggtgt gtacttcata ttgtttgcct tgaaatagtc
     3721 ggcgacgttg agttcggtat cagtacccgc ctgaatacag acggtagcgc catccagttc
     3781 tttcgcgctt tttagccccg ctttatcgtg cgtcaggaag ccaatgccgt cgtaataagt
     3841 gacgccggta aatgccattc ccatcccggc atcgcgagat gaagtccagg tcgtattacg
     3901 ggagagcaaa tccacttccc ctgactgtaa agcggtgaag cgttcttttg ctgtgagtgg
     3961 ggtatatttc actttcgtgt cgtcaccaaa tacagcagcg gcaacaccac gacaaacatc
     4021 aacatcaata cctgaaaact taccgtctgc atcggcataa gagaacctag gtaatccatc
     4081 actgatcccg cattgcacaa aacctttttt ctgaacggca tcaagcgtcg cgccagcatg
     4141 cgcctgattt gcaacggcaa gcagcacgct ggcggcagcc agtgtggcta tcatcatctt
     4201 tttcataatg cttcctgtga ggcgaaaatt atcgttatag gacgtttggg aaacgtcttt
     4261 tacctgtctg tggccatcct cttataagca aaagtagtgc cagcttttgc gcacgatttc
     4321 gcgtcgcaat aagctgagtg tagacaggaa ataataaaaa cagcgccccg caatggtg
//
LOCUS       DADMEQ010000201         4376 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.203, whole genome shotgun sequence.
ACCESSION   DADMEQ010000201 DADMEQ010000000
VERSION     DADMEQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.203"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..368)
                     /locus_tag="JNZ05_003895"
     CDS             complement(<1..368)
                     /locus_tag="JNZ05_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY8566905.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLG"
     gene            complement(483..558)
                     /locus_tag="JNZ05_003896"
     tRNA            complement(483..558)
                     /locus_tag="JNZ05_003896"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(523..525),aa:Thr,seq:ggt)
     gene            complement(565..639)
                     /locus_tag="JNZ05_003897"
     tRNA            complement(565..639)
                     /locus_tag="JNZ05_003897"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(604..606),aa:Gly,seq:tcc)
     gene            complement(756..840)
                     /locus_tag="JNZ05_003898"
     tRNA            complement(756..840)
                     /locus_tag="JNZ05_003898"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(804..806),aa:Tyr,seq:gta)
     gene            complement(849..924)
                     /locus_tag="JNZ05_003899"
     tRNA            complement(849..924)
                     /locus_tag="JNZ05_003899"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(889..891),aa:Thr,seq:tgt)
     gene            1286..2236
                     /gene="coaA"
                     /locus_tag="JNZ05_003900"
                     /gene_synonym="panK"
     CDS             1286..2236
                     /gene="coaA"
                     /locus_tag="JNZ05_003900"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAY8566906.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2265..3230)
                     /gene="birA"
                     /locus_tag="JNZ05_003901"
     CDS             complement(2265..3230)
                     /gene="birA"
                     /locus_tag="JNZ05_003901"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HAY8566907.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGSGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3227..4255)
                     /gene="murB"
                     /locus_tag="JNZ05_003902"
     CDS             complement(3227..4255)
                     /gene="murB"
                     /locus_tag="JNZ05_003902"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAY8566908.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1121 a         1093 c          989 g         1173 t
ORIGIN      
        1 cccagcagga tgtgctcacg agtctgcggc atcgggccgt cagtcgcagc aactaccagg
       61 atcgcgccgt ccatctgagc agcaccggtg atcatgtttt taacatagtc ggcgtgcccc
      121 gggcagtcta cgtgtgcgta gtgacgggtc ggggtgtcgt attcaacgtg agaagtgttg
      181 atggtgatac cacgagcttt ttcttccggc gcgttatcga tctggtcgaa tgcgcgagca
      241 gcaccgccgt aggttttagc cagtacggta gtgattgcag cggtcagcgt tgttttacca
      301 tggtcaacgt ggccgatagt accgacgtta acgtgcggtt ttgtacgttc aaacttttct
      361 ttagacatcg attgtccctc taagacacgg ataaatcggt gatatcacca catcaaccag
      421 gcaacatgcc cgacttgttg aatgcaataa acagaaggaa aaaacaggga ggagaaaaga
      481 agtggtgctg ataggcagat tcgaactgcc gacctcaccc ttaccaaggg tacgctctac
      541 caactgagct atatcagcac atcttggagc gggcagcggg aatcgaaccc gcatcatcag
      601 cttggaaggc tgaggtaata gccattatac gatgcccgca tcctagaact cggctacctg
      661 attttcattc tgcactgaat atcgagagaa gctctcttta ttcgagccgg taagtgaact
      721 tatcgtctcg ggctacgcca tcgcgtggcc gaaattggtg gtgggggaag gattcgaacc
      781 ttcgaagtct gtgacggcag atttacagtc tgctcccttt ggccgctcgg gaaccccacc
      841 ggacttgatg gtgccgacta ccggaatcga actggtgacc tactgattac aagtcagttg
      901 ctctacctac tgagctaagt cggcatcaag tagcgcgcat tctatggaga catgcgagtt
      961 catgcaacta aaaaattgca taatttgttt tattggtcac attttatgct acacgatgaa
     1021 gaaacagccg aacggagggc agattagcac actttttcaa catcattgtg ctcaacaatg
     1081 cgctcctgct aaaccataat tctttttatc agatggaata tctgtcacat tgcttttcaa
     1141 cgatagcttc ctggcagaga ttttttctta ttattcctcc ccatctggtg ttaccctcct
     1201 gcccattaac ccattcaaca gaactgtgac gcgccatggc aaatatcgct ttgccgatag
     1261 agctatgacc gccagaaaca tgcttatgag tataaaagag caaacgttaa tgacgcctta
     1321 cctacagttt gaccgcaacc agtgggcagc tctgcgtgat tccgtaccta tgacgttatc
     1381 ggaagatgag atcgcccgtc tcaaaggtat taatgaagat ctctcgttag aagaagttgc
     1441 cgagatctat ttacctttgt cacgtttgct gaacttctat ataagctcga atctgcgccg
     1501 tcaggcagtt ctggaacagt ttcttggtac caacgggcaa cgcattcctt acattatcag
     1561 tattgctggc agtgtcgcgg tggggaaaag tacaaccgcc cgtgtattgc aggcgctatt
     1621 aagccgttgg ccggaacatc gtcgtgttga actgatcact acagatggct tccttcaccc
     1681 taatcaggtt ctgaaagaac gtggtctgat gaagaagaaa ggcttcccgg aatcgtatga
     1741 tatgcatcgc ctggtgaagt ttgtttccga tctcaaatcc ggcgtgccaa acgttacagc
     1801 ccctgtttac tcgcatctta tttatgatgt gatcccggat ggagataaaa ctgttgttca
     1861 gcctgatatt ttaattcttg aagggttaaa tgtcttacag agcgggatgg attatccaca
     1921 cgatccacat catgtatttg tttccgattt tgtcgatttt tcgatatatg ttgatgcacc
     1981 ggaagactta cttcagacgt ggtatatcaa ccgttttctg aaattccgcg aaggggcttt
     2041 tacagacccg gattcctatt ttcataacta cgcgaaatta actaaagaag aagcgattaa
     2101 gactgccatg acattgtgga aagagatcaa ctggctgaac ttaaagcaaa atattctacc
     2161 tactcgtgag cgcgccagtt taatcctgac gaaaagtgct aatcatgtgg tagaagaggt
     2221 cagactacgc aaataatttg caggggagcg aatactcccc tttcttattt ttctgcacta
     2281 cgcagggata tttcaccgcc catccagggt tttattattc catcctgctc aagtaataaa
     2341 gccccctgtt tgtctattcc gcgtgaaatg ccaaatattt ctttatcacc aatgataagt
     2401 ttcactgggc gattaataaa attatccagc ttttcccagc gcgacagata aggtgccaat
     2461 ccttcttgtt cgaagagttc caacgcagca cgtaattcac gtattagcat ggccgccaac
     2521 gtattacgat caagattgat ccccgcttcc tgcagcgtga tccacccctg attaacgaca
     2581 ctctcttcaa cacggcgcat tgccatgttg atcccggatc caatgactat ttgcgccgca
     2641 tcgccagttt tgccagtcag ctccacaaga atgcctgcca gcttgcgatc ctgcagatag
     2701 aggtcattag gccatttgac acgaacttta tctgctccca gctttcgtaa tacttccgcc
     2761 atcacgatac cgataaccag acttaaacca atcgccgccg ccgggccttg ttccagacgc
     2821 cagaacatcg acaaatataa gtttgcgcca aaaggcgaaa accatttccg cccccggcga
     2881 ccacggccag cctgctggta ttctgcaaca caggcatcgc ccgatttaag ctctccgata
     2941 cgatcaagaa ggaactgatt cgtggagtca ataactggca gcacggctac actaccgcca
     3001 tccagctgac ccaatatctg ttcagcatta agtaactgga tgggctcagg caggctgtat
     3061 cctttacccg gaacggtaaa gacatcaacg ccccagtcac gcagtgtctg aatgtgttta
     3121 ttaatagccg cccggctcat tcccagcgtt tcacccaact gctcgccaga gtgaaattca
     3181 ccgttcgcta acagggcaat caatttcagt ggcacggtgt tatccttcat gaaattgtct
     3241 ccactgcgct cacttcacct gatgcaccaa taaagcggac ttcaggctca agccagacat
     3301 taaatttttc acccactttt tgtcttacat ggtgtgccag ttgcaccaca tcttcgcttt
     3361 ttgcattgtc ttcattaatg agaactaacg cctgttgacg gtgcaccgca gccccaccca
     3421 tttgcatccc ttttagctgg cactgatcaa taagccaacc tgctgccagt tttactgaac
     3481 cacccgcctg ggggtaattt ggtgctgttg gaaattgcgc cagtaatgct ttagccgttt
     3541 cggcagatac aacagggttt ttgaagaaac taccggcatt gccattcact tttggatcag
     3601 ggagtttggt ggtgcgcata tgacacaccg cattaaatac ttgctgtggc gttactgttg
     3661 taggatccag acgagttaag tcaccatacg ttagtacagg ttgccactct tttggcagac
     3721 gcagacctac ggctacaatg gcgaagcgat cctggtattc atgtttaaaa atactgtcgc
     3781 gatagccaaa acggcactct ttggcagtta agcgcacttg cttgcctgtc gccagttcaa
     3841 cacaatcaac ataagcgcaa actcgctgta attctacgcc ataagcacca atattctgga
     3901 taggtgatga gccgacacaa ccaggaatta atgccagatt ttccagacca ggcatacctt
     3961 cctgcaaagt gtattttacc aggcgatgcc agttttctcc ggctcctaca tgtaaatacc
     4021 acgcatcagg ttcatcatga atttcgatac ctttgatccg gttgatgatc accgtgccgc
     4081 gatagtcttc cagaaaaagt acattacttc cttcacccag aataagaacg ggttgtcctt
     4141 ctgcggttgc atgctgccag gcattgagta attgttgttc gtcttcggca catacaatgt
     4201 gctgagcatt atgatcaatg ccaaatgtgt tccagggttt taaggagtgg ttcatagctg
     4261 ctttcctgat gcaaaaacga ggctagttta ccgtatctgt ggggggatgg cttgtagata
     4321 tgacgacagg aagagtttgt agaaacgcaa aaaggccatc ctgacggata gccttt
//
LOCUS       DADMEQ010000202         4339 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.204, whole genome shotgun sequence.
ACCESSION   DADMEQ010000202 DADMEQ010000000
VERSION     DADMEQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.204"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..324
                     /locus_tag="JNZ05_003903"
     CDS             <1..324
                     /locus_tag="JNZ05_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HAY8566909.1"
                     /translation="DDALLQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIP
                     CPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIP
                     SQADF"
     gene            383..586
                     /locus_tag="JNZ05_003904"
     CDS             383..586
                     /locus_tag="JNZ05_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HAY8566910.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENGLPFYTNRSGKPIV
                     SRELFTCNKTLPPHEVEPNFGAI"
     gene            586..1611
                     /locus_tag="JNZ05_003905"
     CDS             586..1611
                     /locus_tag="JNZ05_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8566911.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(1664..1753)
                     /locus_tag="JNZ05_003906"
     tRNA            complement(1664..1753)
                     /locus_tag="JNZ05_003906"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1717..1719),aa:Ser,seq:cga)
     gene            1847..2644
                     /gene="mtfA"
                     /locus_tag="JNZ05_003907"
     CDS             1847..2644
                     /gene="mtfA"
                     /locus_tag="JNZ05_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HAY8566912.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2745..2820
                     /locus_tag="JNZ05_003908"
     tRNA            2745..2820
                     /locus_tag="JNZ05_003908"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2778..2780,aa:Asn,seq:gtt)
     gene            2982..4244
                     /locus_tag="JNZ05_003909"
     CDS             2982..4244
                     /locus_tag="JNZ05_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8566913.1"
                     /translation="MFLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHLELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGSELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
BASE COUNT         1213 a          997 c         1036 g         1093 t
ORIGIN      
        1 gatgatgcac tgttacaatt gcgggaattt atcgacgaaa actccggtga attttttgtt
       61 caggtctggg gaaatggagc caacttcgac aacacgattt tgcgccgttc atacgaacgg
      121 caggggatcc cctgcccgtg gcgttactac aacgatcgcg atgtacgcac aatcgttgag
      181 ctggggaaag ccatagactt cgatgccaga acggctattc cattcgaagg tgagcgccat
      241 aatgcacttg atgacgctcg ttaccaggca aaatacgttt cagctatctg gcaaaaactg
      301 atcccgagtc aggctgattt ttaatgttca accctaattg ccgctaaccg tatatagtta
      361 gcggcggtta tgagatatag ctatgagcag cttattttta accgaagatg aattgctaat
      421 attaacgggc tgcaaatatg caagccacca gcgaaaatgg ttaatggaaa acgggcttcc
      481 gttctatacc aatcgtagtg gcaaaccgat tgtcagccgg gagctattta cctgcaataa
      541 aactttacca ccacacgagg tagagccgaa ttttggtgcg atctgatggg aagacgaagg
      601 aaaaatcctg aacacgaaaa attacctcca aatgtatacc caaataaata tagttatgta
      661 tggaaaccaa catccagaga atctgtaaca ctaaccgcca tcaaggatgg tttagctgct
      721 ttatggaaaa agtatgagga aactgtaaat aatcgcgatc gtgcaatgac attcggtcgc
      781 ttgtgggaaa aattcctcgc cagcgcctat tacagtgacc tcagtccaag aacacaaaaa
      841 gattatctgc aacatcaaaa aaagttgctt gccgtattcg gtaaggtgcc ggcagattcc
      901 ataaaaccag aacacatccg tcgatacatg gacaagagag gggaacagag taaaacgcaa
      961 gccaaccatg aaaaaagcag tatgtcccgc gtttacagtt gggggtatga gcgagggtac
     1021 gtgaaggcta acccatgtgc aggtgtaagt aaattcaagg ccaaaaaccg cgaacgatat
     1081 gtaaccgaca aagaatacca ggcagtatta agcgttgcac ctcttcctgt ttttatcgca
     1141 atggaaattg cctatctgtg tgcagcgagg gtttccgatg tgttatcgct gaaatgggag
     1201 cagattggaa acgacgggat ctttatccag caagggaaaa caggaaaaaa acagataaaa
     1261 gcatggagtc cacgattaca ggcggcgatc gaaaaagcaa aacagttacc aacatccgcc
     1321 tatgtaatca gcaatcaata cggcaaccga tatatgtaca aaggctttaa cgaaatgtgg
     1381 gtagaagcaa gaaatcgcgc aggcaaaatt tcaggtattt taaccgactt cacctttcat
     1441 gatctgaagg cgaaaggaat ttcagactat gaaggaagca gtcgggataa gcaacttttc
     1501 tctggtcaca aaaccgaggg gcaagtgcta atctatgaca ggaaggttaa agtttcaccg
     1561 acacttgatg tcccgttacc tgaaaatatt ccaagaaaat attccaagta attccaagtg
     1621 tgatttttgt cactgactta atgatgtata agtgattgaa ttttggcgga gagaggggga
     1681 tttgaacccc cggtggagtt gcccccactc cggttttcga gaccggtccg ttcagccgct
     1741 ccggcatctc tccgctcaga tggttgccat gatgccagga aatttggcat tttaacagtc
     1801 cctgtccgtg caattttgtt caagtgacga gtttgcgagc aaaacgatga ttaagtggcc
     1861 ctggaaagta caagaatcag cacatcaaac tgcccttccc tggcaggaag cactatcgat
     1921 ccccctttta acgtgtctga cagaacagga acaaagcaaa ttggtcgctc ttgccgaacg
     1981 ttttttacag caaaaacggc ttgttccttt acagggcttt gagctgaatt cattaagaag
     2041 ctgccggata gcacttctat tttgcctgcc cgttctggag ttaggactgg aatggctgga
     2101 tggttttcat gaagtcttaa tttatcctgc gccatttgtg gtcgatgatg aatgggaaga
     2161 cgatatcggt ctggtgcata accaacgtat tgttcagtca ggtcagagct ggcagcaagg
     2221 gcctatcgtt ttgaactggt tggatataca agattctttt gatgcttctg gttttaacct
     2281 gattattcat gaagtcgctc ataagctgga cacccgtaac ggcgatcgcg ccagcggagt
     2341 tccctttatt tcgttgcgtg aggttgctgg ctgggaacac gatcttcatg ctgcaatgaa
     2401 caacattcag gaagaaatcg aattagttgg tgagaatgcg gcgagcattg atgcttatgc
     2461 tgccagtgat cctgctgaat gttttgccgt actttctgaa tatttcttta gcgccccaga
     2521 actttttgct cctcgtttcc cttcattgtg gcaacgtttc tgtcaatttt atcaacaaga
     2581 tcctttgcag agactgcatc acgctaatga tacagactcg ttttcggcga cgaatgttca
     2641 ttaattaaca actttgcaga ttaattaacc aattgaaatg gcttatgaaa tttagtgttg
     2701 acagacaagg taccgctaag taatatgcgc cccgttctca cgattcctct gtagttcagt
     2761 cggtagaacg gcggactgtt aatccgtatg tcactggttc gagtccagtc agaggagcca
     2821 attttctgtt ttcatgcatc cttgcgaatc tttatctgat gttgattcaa caggttagtg
     2881 tgaaaactct tctcggtgca ttttgatttt accctacgca tcgggaaaaa ttggtggtca
     2941 aatctgggat caggttagtt cgataatgga gtgaccccca tatattcctt accgacgcaa
     3001 aaatccgcac cctcaagcct tctgataaac cctttaaagt ctccgattct cacggtctgt
     3061 atctgctggt caagccgggt ggctcccgcc actggtatct caaataccgt attagcggta
     3121 aagaatcccg cattgcgctg ggtgcctatc cagccatctc cctgtctgat gcgcgacagc
     3181 aacgtgaagg tatccgtaaa atgctggcgc tgaatatcaa cccggtacag cagcgggctg
     3241 ctgaacgtgg ctcacgaaca ccggagaaag tttttaaaaa cgtggcgctg gcgtggcata
     3301 aaagtaacag gaaatggtcg cagaacaccg ccgaccgtct gcttgccagc ctgaacaatc
     3361 acatctttcc ggtcatcggg aacctacctg tatcagaact taaaccccgt catttcattg
     3421 acctgctgaa agggatcgag gaaaaaggtc tgctggaggt tgcgtcccgc acacggcagc
     3481 acctgagtaa cataatgcgc catgcggtcc atctggagtt aatcgatacg aaccctgcag
     3541 caaaccttgg cggcgtgacc acacctcctg tcagacggca ctatcctgcc ctgccgctgg
     3601 agcggctgcc tgaactgctt gaacgtattg gggcatatca tcagggcagt gaactgaccc
     3661 ggcatgccgt tctgctgatg ctgcatgtgt tcattcgctc cagtgaactg cgtttcgccc
     3721 gctggtcaga gattgatttc acaaaccgag tctggacgat acccgcgacg cgagaaccca
     3781 ttattggcgt gcgttattcc ggccgcgggg caaaaatgcg aatgccgcat atcgtccccc
     3841 tctcagaaca gtccatcgcc attctgaaac agattaagga tatcaccggt aataatgaac
     3901 tgatcttccc cggcgaccat aacccgtata agccaatgtg tgaaaacacg gtcaataagg
     3961 cactgcgggt gatgggttac gacacgaaaa aggatatctg cggtcacggc ttccgggcaa
     4021 tggcatgcag tgcgctgatg gaatcgggtt tatgggcaaa ggacgcagta gaacgccaga
     4081 tgagtcatca ggagcgcaat accgtgcgca tggcttatat tcataaggca gagcacctgg
     4141 aagcccgcaa agcgatgatg cagtggtggt cggattatct ggaagcatgc cgagaatctt
     4201 atgcaccgcc ttatacaatt ggtaaaaata agtttatccc atagtatact aacgaatccc
     4261 acactctggc aaattaccac cacctctaca ggaggtggtt ctatatagac ttttatcaac
     4321 tttatgacga aaaacacct
//
LOCUS       DADMEQ010000203         4329 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.205, whole genome shotgun sequence.
ACCESSION   DADMEQ010000203 DADMEQ010000000
VERSION     DADMEQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.205"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..157)
                     /locus_tag="JNZ05_003910"
     CDS             complement(<1..157)
                     /locus_tag="JNZ05_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566914.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASK"
     gene            complement(402..932)
                     /gene="cybB"
                     /locus_tag="JNZ05_003911"
     CDS             complement(402..932)
                     /gene="cybB"
                     /locus_tag="JNZ05_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HAY8566915.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1121..2122
                     /gene="gap"
                     /locus_tag="JNZ05_003912"
     CDS             1121..2122
                     /gene="gap"
                     /locus_tag="JNZ05_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY8566916.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATKTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(2164..3603)
                     /gene="aldA"
                     /locus_tag="JNZ05_003913"
     CDS             complement(2164..3603)
                     /gene="aldA"
                     /locus_tag="JNZ05_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY8566917.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3800..>4329)
                     /locus_tag="JNZ05_003914"
     CDS             complement(3800..>4329)
                     /locus_tag="JNZ05_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAY8566918.1"
                     /translation="RTTGRAEATIQVDIAHQFWHIPHEKIWIEDQSTNCGENARFSIT
                     LLNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSV
                     IFINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLK
                     HDAVLIEAMESRSLR"
BASE COUNT         1144 a         1083 c          995 g         1107 t
ORIGIN      
        1 ttttcgatgc actaatagaa ccatcattac agacaaattt gccgcctgcc gtacagtgtg
       61 agatcccccc tttcgaacca gaacagggtt tgcgcccggc atcggcataa aaggaaacaa
      121 gcgttcccat aaacaaaata agtgccagtt ttttcatata acctccatgg tttatcgaat
      181 cttatgtgcg atttgaacgt tatacgctgg tttattttga taccgttgat gagatcaaag
      241 atcgggtgaa ttgattaaaa ataagccaga tggacgtatg ggcaaggatt atggttttat
      301 ttgtcataca aataaatatg ataggcgctt ccgatgtaga cccgtattct tcgcctgtac
      361 cacgggtcgg tttttgtaca ggcgttttct ttaaatatcc ttcaggaacg tttacgcggc
      421 atcatgcgta gaagtgtgtt gtccttccag aaatagtggt gtgccagtgc agccgcagcg
      481 tgcaacccga tgacaaaata tcccagattc gccagcgtct catgccacga ctttaagcta
      541 tcaacccgtt cgaaattggc ctctgaagcg taaggcatcg tcaaaccaaa cgcaaaccac
      601 gggttgcccc ggttatacat catcaccaaa ccaatcactg gcagcgcaat aaacaggaga
      661 taaatcacca aatgtcccaa atgcgccagt cccgtcatca tcggtttcgg cttaggtata
      721 atcggcgggg ttgggtattt cagtcttaac aaaagacgaa ccaccatcag cacgagaatt
      781 gagatgccac aggaaacatg aatcatgttg atgagcggcc gatcgctacg tgggaagaaa
      841 ccacgaaact ccattgcgca atacgctgcg ataaccagca aaaagaccag ccagtgaatg
      901 ctgatttgta accttgagta tttattttcc ataacatttc ctgctttaac ataattttcc
      961 gttaacataa cgggcttttc tcaaaatttc attaaatatt gttcacccgt tttcagtacg
     1021 ttacgtttca ctgtaagaat ggagattgcc catcccacaa tcctggtcta agcctggaaa
     1081 ggatcaattt tcatccgaac gttcctgaca ggagaaaacc atgagtaaag ttggtattaa
     1141 cggttttggt cgtatcggtc gactggtgtt gcgtcgttta cttgaagtca aaagcaacat
     1201 agacgttgtc gctattaatg atcttacttc cccaaaaatt ctcgcctatc tgctgaaaca
     1261 tgattcaaac tatggtccgt tcccctggag cgttgatttt acggaagatt cacttatcgt
     1321 tgatgggaaa agtatcgcgg tttacgccga aaaagaggct aaaaatattc cgtggaaagc
     1381 gaaaggggca gaaatcattg tcgaatgtac tggcttttat acctccgccg ataaatcgca
     1441 ggcgcatctt gatgctggtg cgaagaaggt gttgatttcc gctcctgccg gtgaaatgaa
     1501 aaccatcgtt tataacgtta atgacgacac tctggatggc aacgacacca ttgtttccgt
     1561 ggcgtcatgc accactaact gccttgcgcc gatggccaaa gccttgcatg acagtttcgg
     1621 gatagaagtc ggcacgatga cgaccattca tgcctatact ggcacccagt cactggtgga
     1681 tggcccgcgt ggtaaagatt tacgtgcttc acgcgcagcg gcagaaaata tcattcccca
     1741 cactacgggg gcggcaaaag ccattggtct ggtgatcccg gaactgagcg gcaaactgaa
     1801 aggtcatgcg caacgcgtgc cggtgaaaac aggttcggtc actgagctgg tgtccattct
     1861 cggaaaaaaa gtgactgccg aagaggtgaa taacgcactt aaacaagcaa ccaccaataa
     1921 cgagtcattt ggttataccg atgaagaaat agtctcttcc gatatcattg gcagccattt
     1981 cggttcagtg tttgatgcca cgaaaacgga aattaccgcc gtgggcgatt tacaactggt
     2041 gaaaacggtc gcctggtacg ataacgaata tggcttcgtc acgcagctta ttcgcaccct
     2101 cgaaaaattc gctaaactct gacgcgcaca ggcggaggaa aaaacctccg cctctttcac
     2161 tcattaagac tgtaaataaa ccacctgggt ctgcagatat tcatgcaggc catgtttacc
     2221 atctgcgccg ccaataccgg atttacgcca tccggcgtgg aaaccttgca tagcttcgaa
     2281 gttttcacgg ttgatgtaag tttcaccaaa cttcagccct ttaatggctt tcatcgcgac
     2341 gttcagattt tgggtataga ttgatgaggt caggccgtaa tcactgtcat tagccattga
     2401 gatagcatct tccagcgtgt caaatgcgac aactggcagc accgggccaa aggtttcctc
     2461 atgcataatc gacatttcct ggcgaacatc cagcagcaat gtcggcggat aataatatcc
     2521 tttcccctct accgctttgc cacccaacgc cactctcgcc ccttcttcta ctgcgcgcgc
     2581 cactttttgc tcaacccttt ccagcgccgc ggcgttaatc aacggcccca tcgcaatgtc
     2641 gttgcgttca gcggggttac caaattgaac cgcctgcaac gcttcaccca gccgattgac
     2701 gaactgatca taaatgcctt tctgtacata aacacgttct gcacagttac acacttgccc
     2761 actattaatg acgcgtgaat caacgatggc tttgactgcc agttcaagat cggcatcgtc
     2821 cattacgata gccggtgctt taccacccaa ttccagacac actttggtga tgtttttcgc
     2881 cgcagtcgcc atgatcttct cacctgcaga gacgctgcct gtcatactga ccattgcgac
     2941 ctttgggtta cccgccagtt cttgcccaac ggtttcacca cgccccagta caaggttaaa
     3001 cacgccgcgc ggaaggccta tttcatcgac gattttggcg aatgcaatcg cattgtttgg
     3061 cgtaaattca ctgggtttaa tgacgatggt attaccggtc aaaagagcgg gagccatttt
     3121 gcgggcaatg aggaagaacg gaaagttcca cggcagaatg ccggtagtca caccaagcgc
     3181 acgtttaaac agaagaatat tttctcctgg acgatcgctt tgaataatct cgccctcgta
     3241 acgccgtgcc cactccgcca tgtaatcgat atagtcggca gtaaaagcca cttcgacttc
     3301 agctagctgc tggatcttgc ccccttcttc aacaatcagc gcactgattt cactggcgcg
     3361 ttcgcggatc ccggcggaga ttttgcacaa ccaactggcg cgttcaatag caggcaacgc
     3421 ttcccattct ggttgtgcac gttctgctgc atcgattgcc ttacgggcat cctcggcctg
     3481 accatcgggt atgcgggaaa tgacagcctc tgtagcaggg tttaccacat caatccatgc
     3541 gtctccacgc caggtaacaa actgtccatc gatatacata ggatgttgaa cgggtactga
     3601 catgggcgac tcctgtgatt tatatgtttg ttttcggtga atatattgtt aattaacatc
     3661 aatcatcaga gagacggcat cgggaaagga caattctgtg gagggcttca taaaaacaag
     3721 tgtaaaagga ataaaaggca gaaataaaag tcaggagtca tgcccgtttg caaacaagtg
     3781 gttcacaaac gggcaatttt taacgtaacg agcgactttc catcgcctcg atgagcaccg
     3841 catcatgttt cagcgtttgc catgcatgga tgacttctgc cggaaaatca acgtgaacga
     3901 taaaatctcg cccgcgggga ccgtagccat cgctatcatc gtgtaaacgc ggcagctcgc
     3961 cagtgagtag tgagagataa cgctcaaccg gccataatcc ttgtaactga ttaataaaga
     4021 ttacactgtc tgcgttattt cctaactgag gaacgaatcc gggataactt aaccagcgtg
     4081 gtgcatcggg attgtccccg gtcatacggc ggaacgttgc catcgtgcgc cgctgcatgg
     4141 tggggtcctg tacaacgata gccgtatgaa ctcgttctac ggcctgattc aatagcgtga
     4201 tgctaaagcg tgcgttttca ccgcagtttg ttgactggtc ttcaatccag attttttcat
     4261 gcggaatatg ccagaactga tgagcgatat ccacctggat ggtcgcttct gctctgccag
     4321 tggtgcgga
//
LOCUS       DADMEQ010000204         4323 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.206, whole genome shotgun sequence.
ACCESSION   DADMEQ010000204 DADMEQ010000000
VERSION     DADMEQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.206"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..158)
                     /locus_tag="JNZ05_003915"
     CDS             complement(<1..158)
                     /locus_tag="JNZ05_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY8566919.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRFSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="JNZ05_003916"
     CDS             complement(308..469)
                     /locus_tag="JNZ05_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566920.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(466..669)
                     /locus_tag="JNZ05_003917"
     CDS             complement(466..669)
                     /locus_tag="JNZ05_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAY8566921.1"
                     /translation="MVERTMAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAIT
                     PYTLMQAIAAEGDVVVSGATEPE"
     gene            914..1249
                     /gene="iraM"
                     /locus_tag="JNZ05_003918"
     CDS             914..1249
                     /gene="iraM"
                     /locus_tag="JNZ05_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY8566922.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1661)
                     /locus_tag="JNZ05_003919"
     CDS             complement(1530..1661)
                     /locus_tag="JNZ05_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 domain-containing protein"
                     /protein_id="HAY8566923.1"
                     /translation="MIAYCFCEWHCSSRIMSVPADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(2161..2237)
                     /locus_tag="JNZ05_003920"
     tRNA            complement(2161..2237)
                     /locus_tag="JNZ05_003920"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="JNZ05_003921"
     tRNA            complement(2339..2414)
                     /locus_tag="JNZ05_003921"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2558..3379)
                     /locus_tag="JNZ05_003922"
     CDS             complement(2558..3379)
                     /locus_tag="JNZ05_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY8566924.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3394..3750)
                     /locus_tag="JNZ05_003923"
     CDS             complement(3394..3750)
                     /locus_tag="JNZ05_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY8566925.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3763..>4323)
                     /locus_tag="JNZ05_003924"
     CDS             complement(3763..>4323)
                     /locus_tag="JNZ05_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY8566926.1"
                     /translation="AELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPG
                     EQTATSILKQRTKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRSWVNE
                     KYKRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLA
                     FEEKHGSQVDLIFRFLDHAFATGVLG"
BASE COUNT         1185 a         1177 c          856 g         1105 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaacc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaccatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgc
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgtc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattgca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccattc acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaac
     2461 ccgctcagcg gcgggttgta gaaactcttc taacgtcagg cataaaacgc ccatcattat
     2521 gacgaattta ccacagattc cggaaaaatc aaccttgtta cctagttacc ttttttaact
     2581 gccgctcagc ccatgcttct tcaatatcaa accgggtcac cagcgcatca taaaatttct
     2641 taactgtttt ttcccatgac gcgcgtgtta tctggtttgt cacctcgcat atagcattaa
     2701 atgcctccgt tgatggtagt ctttcatagc cacgaccacc acaacgctgg cagtctctga
     2761 taacaggcat accacgtttt accgactctt cacgatgaat ggcgacacca cgcccacggc
     2821 aatccttaca ggcggtggaa acctcaccct ttccgccaca ctccggacag gcaactttta
     2881 ccacctccct gacttttttc cattcttccc agtaagacgg atacacacct ttcgtgcact
     2941 ttgcccatac cggcggctta ccatccggat actggatctt gtttgtaaaa actatgcttt
     3001 caataaattt ttccccatag caacaagggc actgcttttt actcgctgcg ctgcgggcat
     3061 aatcctcaaa agcgtacgaa gccataatgc gcatcactac cggttttatt tctgccggaa
     3121 gttttctcaa cgccgccaca cgatcgcacc gactgagtgc ataatctgcc agtaattctg
     3181 ttgcccgcgc cctgtcattc atactgatgc ccattttccc caggaacgca gaaaaaccca
     3241 tctcagcccg attctgtgtc atgccctgcg cggccatcac atcagtgata ctcagcgcat
     3301 cttttgacgt tgaggccgat gcatcggtca ggccagggga ttttggggag tagtatttcg
     3361 gtaaatcttc cagtttcatt ttttgacctg ctcttaatgc attatggggt aaatcttcac
     3421 ccccagacgt ccaccagata ctggctgacc acgaacgata ttgatttcat caaactgctc
     3481 atcgtccatt aacactcccg catgcgtcag cgcatccagc ggtgctttca ggatattgtc
     3541 caggtcgcga cgacgcttat ccggtggctc tgcaatcacc tttatcgcca gccttccgga
     3601 caggcttaat ttcagccgct gctggcgaac aataagcgcc acagcccggc gataacgctt
     3661 tccctcctcc gagataaaat atgtgctgcc acggcgtcgc cagtaagtgt tcaccgtcgg
     3721 cgggtaaggt aaaaccaaat ctatgagcat cagtcacctc ttttacccaa gcacgccagt
     3781 tgcaaaggcg tgatcaagaa aacgaaaaat taaatcaacc tgagaaccat gcttttcttc
     3841 gaacgccaga ggatccgcat gaagctcgtt gtgatgctcc cgacacagcg gtagcgtgaa
     3901 aatatcgtga gattttgtcc ccattccgcc ctgaccatga ccaatcaggt gatggggatc
     3961 gtcggctggc ttaccacaac acgcacacgg ctgtgtcttc acccagcgtt tgtatttctc
     4021 gttaacccag ctgcgacgtt taggtcgttt catgaaagat tccggagact caggatcaac
     4081 ggcaatgctg accaccgtct tttcctgttg tgggttctgt tgctggtggg cgtgaggcag
     4141 cggcgcaaga ttttttgtgc gctgtttcag tatgctggtg gcggtctgct ctcccggtac
     4201 gatgtcgctt tcacggtaca ttgagcggat tttttccgca cgcaacccca gcgaacgacg
     4261 taataccgct tccggtagcg cgtccgccac ctgattgcgg accgcccacc aggataattc
     4321 agc
//
LOCUS       DADMEQ010000205         4252 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.207, whole genome shotgun sequence.
ACCESSION   DADMEQ010000205 DADMEQ010000000
VERSION     DADMEQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.207"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..546)
                     /locus_tag="JNZ05_003925"
     CDS             complement(<1..546)
                     /locus_tag="JNZ05_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566927.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDGI"
     gene            complement(1208..2194)
                     /locus_tag="JNZ05_003926"
     CDS             complement(1208..2194)
                     /locus_tag="JNZ05_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566928.1"
                     /translation="MMTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSSTGFTRGTLQQDALNICDNVVRTINSKMV
                     DVLESSKLLKTVELPVLTGSLTAKADTIMDALYENTESSFGSEVTDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDIAYYNGADRGVFMMAKRFTALSFGCFRHDGENITV
                     VLSRDGNTQSHDLEILGKVFVVAEAATTVKMGTGSAAAVLPVVKRLSFTKTS"
     gene            complement(2276..2767)
                     /locus_tag="JNZ05_003927"
     CDS             complement(2276..2767)
                     /locus_tag="JNZ05_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566929.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSRESMDLLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAERIAKELASQKE
                     RTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(3062..3802)
                     /locus_tag="JNZ05_003928"
     CDS             complement(3062..3802)
                     /locus_tag="JNZ05_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020301031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HAY8566930.1"
                     /translation="MKHEVVLPVIVDGYHWACNQDRMWNLNELHKELELPSAKEPGQW
                     RSSVRTLLDQDANLHVAHGGSTPGTWATEEAAIAYAMWVSPDFYLKVIRAFIALRNDK
                     VSEAKALAQDSKELKGLKPIARNWLEKLDNPKQGHTLTECLKNLDFRLGTKLVSAKAL
                     NAVLKSGRIANPFYSRKLDGRGQPVFDISSGGWMTSFNPKGLENGYYRLQNNHYNGQE
                     GLKVLTKGYEWLKSNKVELVRLCAKGGL"
BASE COUNT         1097 a         1005 c         1064 g         1086 t
ORIGIN      
        1 aattccatcc agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg
       61 cgtagacata taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg
      121 gtctgatact aatgccatat agccccataa actatccctc agccagacag catcatagtg
      181 ggtttctgcc gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg
      241 tttctccgtc aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgaatttc
      301 gtctttggta tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaagacg
      361 ataattagga tacttatctg gatttaacga taaataactt ttatctgttg ctgcccccct
      421 ggaattaaga atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt
      481 gccgcacgaa aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata
      541 atccatcaat cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg
      601 tattttacct gggttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa
      661 aatggggaag tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag
      721 aaaatcagat acagcaaaaa tggcagattg atcaggtgaa gataaattgg agcgggcgaa
      781 gggagccgag gacaactagc taacttcttg tttcttaaag actgatgaat ccagtggtta
      841 cagtgatggt tacacatgga gaggctaaag gtcaagactt gaagtacgta acattcccac
      901 aaaaggaata gaacacagtc acggactacc cggccctttg gtctgactga gggtcgggct
      961 acggggtaaa tcgtcaggct tattgtatta gatggtctct cacatttttc agtcaaaaac
     1021 tcatcgtact ttaggccaga ccatcagcat gactatcaga gccagcttta gcaggaagac
     1081 cacgagatag actttgtgta ggtccattag gtcctcctct ttccgcaccc acgaactaga
     1141 ttactagtac aaaataaagg ccagacatcc agttacggaa gtctgaccaa taagtcgtta
     1201 ttgttagtta gcttgtctta gtaaagctca ggcgtttgac tacaggcagg acagcagcag
     1261 cgctgccagt ccccatctta acagtcgttg cagcctcagc gaccacgaag accttaccga
     1321 gaatctcaag gtcgtgagac tgggtgttgc catctcggga taagaccaca gtaatattct
     1381 cgccatcgtg acggaagcaa ccgaagctca gagccgtaaa gcgcttagcc atcatgaaga
     1441 cgccacggtc tgcaccgttg tagtaggcga tgtctgtacc cagcatatcg acaatagcgt
     1501 cttcgccacc gaagccctgc ttagcagcca gacgggaaag agccttgagg tgtgactcat
     1561 gggcgatgat accgtaatca gtaacctcag aaccgaaaga agactcggtg ttctcataca
     1621 gagcgtccat gatagtgtct gccttagcgg tcaggctacc agtcaggacc ggaagctcta
     1681 cggtcttcag cagcttagag gactccagaa cgtcaaccat cttagagtta atggtacgga
     1741 ccacgttatc gcagatgttc agagcatcct gctggagcgt cccccgagtg aaaccagtag
     1801 aggacttctg gtcccagagc tttgtctgat gagacacgag gatagcctgt tcaatcggct
     1861 caacggtgat ttcagtacca gacggagcgg tcacgacttc aggtcgctta ccaccacgga
     1921 caggaacgac cacagagcgg tcagccagag tgtgagcaat gattggcgtc ggtaagctgt
     1981 tacgggtcgg caggacttct acagggaacg gaatgttttc ctgagcgtct ttaagtacac
     2041 cgttcagcat agccttaacc gtcttagcta actggagggc atagacctgt gggtcagcgt
     2101 tagtaggcag accttcattg acctgaatgt tgtcggtgtc ggtgatacgt actggctcaa
     2161 taccgaactg gaaagcgtct ttaatcgtca taataatcaa tctcctaaat tagtttaaat
     2221 actgtgagtc ctcaaaactg aggagtccct agcatcgggt cagggactgt gtgttttaca
     2281 ggctgtccat ccagttaccg cctgactcaa ggtcagcgac gatagcggca cgggagcggc
     2341 cagcggaatg ggtagcactg cggaggtttt caccataggt ccggcgttca gctttgacct
     2401 gttcgcggcg ctgtagctct ttttgccatt cgtcttcggt catgaacatt tcgcgggtac
     2461 gctctttctg agacgctagc tctttagcga tacgctcagc ttcagctcgt ttgttcatct
     2521 ggcggcgttg gtaggcggtc atcaggtcat tactgaagaa gttaccaatg gtcacgccag
     2581 cttcagccag agcttccagt aaatccatag actcacggct ggtagacggc aggccggaca
     2641 ggtagcccgg aatgagggtc ttgatagcga gcgacaacgg ataggactga ccgtccagac
     2701 tcacagtgtt ttcataaagg ttgatgtcgg tgactgacag cttccagttg ttgttgttga
     2761 acatcatagg taattctcct cttgagagtt gagactatcg gtatcgcagg cacagtaagg
     2821 cctactggtc tccccgcatc ggtcatctca aaaggtgttt aaattaggta ttgagtaggt
     2881 gagctactaa cggttaagcg gcagtttcat agcgaccagt tgggctatta gctcgtctcg
     2941 ggattggtgc tggcgggtct catcttccgg gtctagacca agctctctga ggaggacttc
     3001 agtccggtag ccacggtaac ggagagtcat gcggccctca ggtgtgcgcg ggtcaatcat
     3061 cttagagtcc tcccttagcg cacagacgaa ccagctcgac cttgttggac ttaagccact
     3121 cgtaaccctt ggtgaggacc ttaagcccct cctgaccgtt gtagtggtta ttctgaagac
     3181 ggtaatagcc gttctccaga cccttcggat tgaagctggt catccagcca cctgacgaga
     3241 tgtcaaatac aggctgtcct cgaccgtcca gcttgcgact atagaaggga ttagctatac
     3301 gccctgactt caatacggca ttaagagcct tggctgatac cagctttgta cctaacctga
     3361 agtcgagatt cttaagacac tctgtcaggg tgtggccctg cttcgggtta tccagctttt
     3421 ccagccagtt acgggctata ggcttgagac ccttaagttc cttggagtcc tgcgccagcg
     3481 ccttagcttc cgacactttg tcgttacgta aagcgatgaa agcacggatg accttaaggt
     3541 agaaatcagg tgagacccac atcgcatagg caatagccgc ctcttcagta gcccatgtcc
     3601 ccggggtcga accaccatga gcaacatgca aatttgcatc ttggtcaaga agcgtcctga
     3661 cgctagaacg ccactgaccc ggctccttag cagatggaag ctcaagctct ttatgtagct
     3721 cgttcaggtt ccacatacgg tcctgattac aggcccagtg atagccgtca acgatgacag
     3781 gaagaactac ttcatgcttc attaagggtt gagttgacat atcgttgtac tccttttgtt
     3841 atgcctcctg aagtcacacc ctgagagcct actcaagccc ccaacgatag ggagactcac
     3901 aggcaacagg agacatacag aaagacttga ggtaattgtg atgtggtctg cgatgaccat
     3961 agatatgaag gttcgacagc gtgtcaggac gaccagatgc tagaagctga tagacttcaa
     4021 gaggagaaga acttctaggg gctggaggtc tgacagaggc tgtcttgcct tgggatgtct
     4081 tcggagaaag acttaatgtc gggagagctt ccgcataact tgacgcatca agaccagagg
     4141 cggaatgtat ggtctctaca gtgagggtgt aagatttgct acctgataag gccataatct
     4201 gacctccagt tagacccgaa gtatggagac cacatcatag cgaccatagt tt
//
LOCUS       DADMEQ010000206         4230 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.208, whole genome shotgun sequence.
ACCESSION   DADMEQ010000206 DADMEQ010000000
VERSION     DADMEQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.208"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..512)
                     /locus_tag="JNZ05_003929"
     CDS             complement(<1..512)
                     /locus_tag="JNZ05_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY8566931.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFAR"
     gene            complement(723..2120)
                     /gene="ccp"
                     /locus_tag="JNZ05_003930"
     CDS             complement(723..2120)
                     /gene="ccp"
                     /locus_tag="JNZ05_003930"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HAY8566932.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLVLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2525..4174
                     /gene="treF"
                     /locus_tag="JNZ05_003931"
     CDS             2525..4174
                     /gene="treF"
                     /locus_tag="JNZ05_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HAY8566933.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1085 a         1076 c          991 g         1078 t
ORIGIN      
        1 cgggcgaatt tatgccagca gatttgtacc ggaccgcaca ctaggtttgg tttatccgtt
       61 ggtttgcctg cagcttccat acgcttgcgc caacgccatt tcatcgccat cccgccgagc
      121 atacaggcct cggaagaacc aatggtgttg gtgccaacgg cctgaccatt tttcggcgca
      181 ggcgcatgcc acagatcggc aaccatattt acgcaacgca ggtcgatggc tgcggattgc
      241 ggatattctt ctttgtcgat ccagttttta ttgatcgaca aatccatcaa tttatggacg
      301 ttttcgtcgt cccaggtctg gcagaaagtg gctaggttct gacgagcgtt gccatcaaga
      361 tataattcat cattgataat ctggaatgcg acatcatcgc gcatttcgtg cagcggaaat
      421 cgttttgact ccgcgatagt agaaatggcc tttgcgccaa aacgtgaatc gagtagttct
      481 gagcggaaat ccgttaacag cttctggtcc atttcgaact ccttaaattt atttgaaggc
      541 aataaaaaag taggatttat ccgcaatgga agcaaggcat tacaggctta atttaaataa
      601 caaaatccta agcagaaaaa cgacttttta taatataaac atttaacatg ataatattaa
      661 aaacagactt tattatcgta ataaaaatcg cccaaacatt cctgttcggg cgatcaaatt
      721 aattattgtt tatcctgcat atacggcgtg tacaccccgt tcagactgtg caggaaagct
      781 acgatatcat ccacatcctc ctgcggcagc tctttgccta cctgatagcg cagcatcagt
      841 ttcaccgccc cgtccagcgt cggcacgtca ccgcgatgga agtacggtgc cgttaaagca
      901 acgttacgta aaccgggtac tttctgacgc aatttatcac gctcttcttt agtcacattc
      961 atacgaccaa tatccgccgc cgtaatttcc ccaaagttaa agtctttttt cagcaccaac
     1021 ggttcaaagg aacgtccgcc gagaataata ccaccatgac aagttgcaca tttattatct
     1081 ttaaataatt gatagccttt tttctgttgc gccgtcagcg cattttcatc tccacgcaac
     1141 catttatcaa atggggaatc cggcgtaatt aatgttttct caaattcagc aatggcatca
     1201 gtaatatttt cgccactgaa accttgcgga taaacttcga ggaactgcgc tttaagctgc
     1261 ggatcttttt ccagcttagc aataatttcg tcccaggatt tcgacgccat ttcaatcggg
     1321 ttcaacggcg gtccacctgc ctgatcctgc aatgttgccg cacgaccatc ccagaactgc
     1381 tcaacgttaa atactgagtt aaataccgtc ggcgcattaa tcggcccaac tgcgccacca
     1441 acaccaatcg atgtttttct gccatcgacg ccccccgcat tcaacgtatg gcaatgagcg
     1501 catgaaatgg tgctatcagc cgataaacgg ggatcgtgat acagcgcaaa gcccaacgcc
     1561 actttttgcg catcggtagg cagtttttgc gggatgggct gcaccggttc attgcgatgc
     1621 tccggagcag tatcattgct ggcgtaatat tccgcgcgct gttttgcaat ccaggccagt
     1681 atttccgccc gctcttcatc actcacctta cccgcccagt gtagcgcggt ataacgcgtt
     1741 ggtggcatag tttcatactg catcacccat tcaatcttat tcagatcgct ttgcgaaacg
     1801 ggtttatcag ccagcagtgc cgcacgcacg gcctcgaggt taaaagattt atatccaagc
     1861 ttaatgtcgt aatccatcaa ctgtttcgcg ccaggaatat aataataggc gggtaattct
     1921 gccgaaggcg tgtggcaata gtcgcatcct ttttcgcgga gaaagcctaa aaccttatta
     1981 ttttcactga cagcagatgc ctgaacatcg gcctgtttac tgcgtttatt atcgtggtac
     2041 cacacataac cagataaccc taaatagcaa atcgcgacgc cagccaggcc gatcgcggta
     2101 atacgtgaga ccattttcat tattcttccc tcacggtggt gagttattgt gacccagaaa
     2161 attttccaga atgctgcaaa cgtctctgcg agacaaagcc atcataaaaa acagggagat
     2221 acgctttttg ataggaatca atcaattaca tgaatataaa ctatcaataa gatagcctgt
     2281 gcctattacc cagatcagga atggcatatc atgattgcgg cgagagcagg attgaatgtt
     2341 ctgaaaatga aaaacaacac caaatccgta tttttataaa ttccattgat attagtgcgt
     2401 tatatttttt gacaagttaa taaacgtgat ccaccgcacg ctttgtcgcc caccaggcgg
     2461 agcgaatgac tacccttaaa gaaaagcccg ataattagcg acgaatttcg gaggttggat
     2521 ccttatgctc aatcagaaaa ttcaaaaccc taatccagac gaactgatga tcgaggtcga
     2581 tctctgctat gaactggacc cgtatgaatt aaaactggat gagatgatcg aggcagaacc
     2641 ggaacccgag atgattgaag ggctgcccgc ctctgatgcg ctgacgcctg ccgatcgcta
     2701 tctcgaactg ttcgagcatg ttcagtcggc gaaaattttc cccgacagta aaacctttcc
     2761 cgactgcgca cccaaaatgg acccgctgga tattttaatc cgctaccgta aagtgcgccg
     2821 tcatcgtgat tttgacttgc gcaagtttgt tgaaaatcac ttctggctgc cggaggtcta
     2881 ctccagcgag tatgtatcgg acccgcaaaa ttccctgaaa gagcatatcg accagctgtg
     2941 gccggtgcta acccgcgaac cacaggatca cattccgtgg tcttctctgc tggcgctacc
     3001 gcagtcatat attgtcccgg gcggccgttt tagcgaaacc tactattggg attcctattt
     3061 caccatgctg ggactggcgg aaagtggtcg ggaagatttg ctgaaatgca tggccgataa
     3121 cttcgcctgg atgatcgaaa actacggtca catccccaac ggcaaccgca cctattattt
     3181 gagccgctcg caaccaccgg tttttgcgct gatggtggag ttgtttgaag aagatggtgt
     3241 acgcggtgcg cgccgctatc tcgaccacct taaaatggaa tatgccttct ggatggacgg
     3301 tgcagaatcg ttgatcccta atcaggccta tcgccatgtt gtgcggatgc cggacggatc
     3361 gctgctcaac cgttactggg acgatcgcga cacgccgcgt gacgaatcct ggcttgagga
     3421 cgttgaaacc gcgaaacatt ctggtcgccc gcccaacgag gtgtaccgcg atttacgcgc
     3481 gggagcggcc tccggttggg attactcttc ccgttggctg cgtgatactg gtcgtctggc
     3541 gagcattcgt accacccagt tcatccccat cgatctgaat gccttcctgt ttaaactgga
     3601 gagcgccatc gccaacatct cggcgctgaa aggcgagaaa gagacagaag cgctgttccg
     3661 ccagaaggcc agtgcccgtc gcgatgcggt aaaccgttac ctctgggatg atgaaaacgg
     3721 catctaccgc gattacgact ggcgacgcga acaactggcg ctgttttccg ctgccgccat
     3781 tgtgccgctc tatgtcggca tggcgaacca tgaacaggcc gatcgtctgg caaacgccgt
     3841 acgcagccgg ttactgacac ctggcgggat tttggcaagc gagtacgaaa ccggtgaaca
     3901 gtgggataaa cccaacggct gggcaccgtt acaatggatg gcaattcagg gatttaaaat
     3961 gtatggcgat gaccttctgg gtgatgaaat cgcgcgcagc tggctgaaaa cggtgaatca
     4021 gttctatctg gaacagcaca aaatgatcga gaaataccat attgccgatg gtgttccccg
     4081 cgaaggcggc ggtggcgagt atccgttgca ggatgggttt ggctggacta acggtgtggt
     4141 acgccgttta attggtttgt acggcgaacc ataatatttt tacagccagc cgctaacttc
     4201 ctgctggctg taaaattatc ctcttcagga
//
LOCUS       DADMEQ010000207         4114 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.209, whole genome shotgun sequence.
ACCESSION   DADMEQ010000207 DADMEQ010000000
VERSION     DADMEQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.209"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            245..1200
                     /locus_tag="JNZ05_003932"
                     /pseudo
     CDS             245..1200
                     /locus_tag="JNZ05_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842986.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1197..2102
                     /locus_tag="JNZ05_003933"
     CDS             1197..2102
                     /locus_tag="JNZ05_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HAY8566934.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASVAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLILADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            2099..3169
                     /locus_tag="JNZ05_003934"
     CDS             2099..3169
                     /locus_tag="JNZ05_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAY8566935.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECDTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISILNGFDNLMAQPLLSDEPLTALLDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGDNSGGLLSFTLAHTDAWRQQGYEDTMLTMEHIRKPLAARQVLSRSETVL
                     QKSLEITEEADLALRNAMARIK"
     gene            3305..3982
                     /locus_tag="JNZ05_003935"
     CDS             3305..3982
                     /locus_tag="JNZ05_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566936.1"
                     /translation="MQLPVKLLMSLISLVSVIARAGKYKNYIRDEIKYWRYTSYKGGK
                     FPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNVEAASVKQALSCDPNARA
                     EQPGACPTTYELYEGDAAYKAALDKALKPVGLSGMFGKGGYMDGPGGNVTPVTINGTV
                     WLQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVK
                     NYVAAVN"
BASE COUNT         1070 a          970 c         1139 g          935 t
ORIGIN      
        1 ctcacttatc gatcaactga taacggaagt catactgagt tcacactcca gagctgcggc
       61 actggcggtt gattctgccg ccgcaaaact gatgcagaat gcagacaata tcagtgaata
      121 cctgtcgtta caccatcagg ggctgatgca aagtattcag tcactgcagg cgcatatcac
      181 cagcctgctt gaggatatcc gggaaatagc agactgtcag gagcaggtga ccgctgatgt
      241 cagaatggcc atggaggaga tcgatgccag aacccgggaa ttactgacgg ggtctgcacc
      301 tcactggaag aggagctgaa tgactatttc agaagcggta aacgcaaaga acagcaaatg
      361 ctggaggaag aaaattcagc gcagccccgg gagagaaacg cattcgcttt ctttcacgat
      421 attttcggaa ccggaaatca gcatgacagg atgcgggact ttgatccgga cagccccgaa
      481 ataaaattca gtgatcgcag ggaagccctt gaactgatga cgcaaatcga atcgaccgtg
      541 accagcctgc accgagaggc tgaagcacag ttccggcctg agctggagaa aatcgtcagc
      601 gggattgaaa caggttttcg tggcacggcc ctgtacgcca cagaaaacat tgccggtcgc
      661 atcaataccc gcctggagga tgagggcttt accgtaaaaa tcagttttcc ggcagtcagt
      721 cagttacaaa cccggctcgc ggtaaaaaca aatctgagtg cgcttatgga ggaaagaacg
      781 gagaccgtca cccgtcgccg tcggcagcgt ggtttatggg gaaagatttg tggagcgttt
      841 ggcaccagtg actggggctg ggaaacctat aaagaggatg tgagccgcag tgttatcaat
      901 atcaacaccg tcaggaagga agttatgtca ctgacccggg catatttcgg ggagctgcag
      961 gcatccattg agcaggatat taaccagccc gtccgccagg aaatcgatgc ctttttctgc
     1021 gcattcaggg agaaagttga acaactgcgt aacacgctga ttcagagctc tgaagatcat
     1081 aaacgcgatc agcaggcaca ggaacggctt accaggcgac ttcaggcatt aaatgaaagg
     1141 gttcctgagc tcattactga cagtaaggcg ctgagggaag agctggagac aatgctgtga
     1201 cttcaccatt tattcaacaa attgctgata accgggtatg tcaggtactc acctgtcttc
     1261 ctgaaaaatt tgtggttgat ttcgcaaacg gaatcgatgt tgcacaggag catatccgca
     1321 cggccggaga gcgcacgttc ttccggcgtt taaaagaggg actgaccggc gaaggcgctg
     1381 cacggcagaa tgccatcaat gcttcggttg cacagggcgt tgaggcgtcc ctgcgctggc
     1441 tgacggagct gacaacgtca cttgccacca caaactacgc gattacccgg gtaaacgaca
     1501 gggtcagttc actggtcagt gatactgcca ggttggcgca ttattccgca gatacacggg
     1561 agcagttact catcctggcc gatcaggttc accataaact gaatcatctt gaagaaaaac
     1621 tccaccgtgt tgaccaggtt cagcgggcgc agttacatct tgagcagata ttctcatggt
     1681 ggagcgccgg gcgatacgcg tcattttccc ctgccggacg ttgttatgtg gcacttgaag
     1741 agcttcgctg gggcgcgttt ggtgatgtga tacgtcaggg cgaaacaggc caggttaacc
     1801 agctactgga tattctcaga cataaagcat taacgcagat ggcacaggag agtggcggta
     1861 gtgcaaccgt gcgtctgaac actctggact ggcttggtgg tcagggccgg gagcaggcag
     1921 ataacgagtg gcatgacgcg attaactggc tgggagactg gtgcagtgaa gagcagcatc
     1981 cggtgatctg gtcaaccacg caggctgcag aacacttacc ggttcgcatg cctcgcctct
     2041 gctctgcaga acgcctctct gaaagtatgg tcgatgaaat atttcagaag ggggctgcat
     2101 gagtacagaa atgaaaacgg ggctggtgct gtccggtggc ggggcggtgg gcgcttatca
     2161 ggcgggagtg gttaaggcac tggcagagtg tgatacacag atcagcatgg tttcaggggc
     2221 cagcattggc gcattcaatg gtgccattat cgcggcctct cccgatctgt cagaagctgc
     2281 cgtacgcctg gaagcgctct gggatcatct ggggaataat caggtgctgt cggtaaaccg
     2341 attggtttac ttttcattgc tgaaaaaatt gttccaggca atgaacctct gccagatccc
     2401 cggacgtgca ggagcactgc ttacgacgct tcttcgccat atatcgatac tcaacgggtt
     2461 tgacaatctg atggctcagc cgttgttgtc agatgagccc ctgacagcgc tgttggatca
     2521 ttatcttgat actgatgctc tggcagacgg gctaccgctg tatgtgtcgc tgtaccccac
     2581 agaagggggc atgcaggata ttattgactg cattcgtgct gaactgggtg ccggaaccac
     2641 gaaaaacgcc gtttttcagc atatccagag cctgccccgc ggacagcaga aagaggctct
     2701 gcttgcgtca gccgcgctgc ccctgctgtt ccgtccccgt gaggttcagg ggacaatgtt
     2761 cggtgatggt ggtatgggag gatggcgaaa tatgcaggga aatacccctg tgacgcctct
     2821 ggtcgatgcc ggatgcaata tggtgattgt gacgcatctg agtgacggtt ctttatggga
     2881 tcgccaggct tttccggaca ccacaatcct tgagatccgt ccccggaaaa ggctgaaata
     2941 tgcaggtgat ggcgacaaca gcggcggtct gctcagtttc acgttggcac ataccgacgc
     3001 ctggcgtcag cagggctatg aagacacgat gctgacgatg gagcatatcc ggaaaccgct
     3061 ggcagcacgt caggtactgt cccggtcaga aacggtgttg cagaaaagtc tggagataac
     3121 ggaagaggca gatttggcac tgagaaacgc gatggcccgg attaaataaa gacgctccaa
     3181 agaaacgcca ccgcctgtgg tggtgttttt cctagctggt gttattgaaa atattttcag
     3241 atatatatta cctgagagaa agttttaccc ctcacctgtc aattcactca ctggaaattt
     3301 aattatgcaa ttacctgtaa agttattaat gagccttata tctctggtca gcgttattgc
     3361 acgtgccggg aaatataaaa attatatccg ggatgaaata aaatactggc gatatacatc
     3421 atacaagggt gggaaatttc cggaaggttt cactgatgag aaattttcca gcgccattta
     3481 caacggaaga atatttacaa tgaaacgttt acataccctg atgttatttc tggcggttct
     3541 gtttactggc tttaacgtgg aagcagcgag cgtgaaacaa gcgctcagct gcgacccaaa
     3601 cgcccgggct gaacaacctg gagcgtgtcc aacaacgtac gagttgtacg aaggtgacgc
     3661 tgcctacaaa gctgcgcttg acaaagcatt aaaaccggtc ggactgagcg gcatgttcgg
     3721 taaaggcggg tatatggatg gccctggcgg aaacgtaacg ccagtaacca ttaacggtac
     3781 agtctggctc cagggcgacg gttgcaaagc caatacctgc ggctgggact ttatcgtaac
     3841 actctataac ccaaaaaccc atgaagtcgt tggctaccgc tactttggtt tagatgaccc
     3901 ggcctacctg gtttggttcg gcgaaattgg cgtgcatgaa ttcgcgtatc tggtgaaaaa
     3961 ctacgtagct gcggttaact aatttaagga gtctgtcatg aaaactttta tcaaaacttt
     4021 actcgttgct gtaactattc tgttctctgt cttcgctacg gcgaaacaag taaaactgcc
     4081 aaacaacatc aaatacgtta atactacaga ggcg
//
LOCUS       DADMEQ010000208         4061 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.210, whole genome shotgun sequence.
ACCESSION   DADMEQ010000208 DADMEQ010000000
VERSION     DADMEQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.210"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..113
                     /locus_tag="JNZ05_003936"
     CDS             <1..113
                     /locus_tag="JNZ05_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HAY8566937.1"
                     /translation="QAQVDRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(314..1171)
                     /gene="sitD"
                     /locus_tag="JNZ05_003937"
     CDS             complement(314..1171)
                     /gene="sitD"
                     /locus_tag="JNZ05_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HAY8566938.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1168..2025)
                     /gene="sitC"
                     /locus_tag="JNZ05_003938"
     CDS             complement(1168..2025)
                     /gene="sitC"
                     /locus_tag="JNZ05_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HAY8566939.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     THKALEDRS"
     gene            complement(2022..2849)
                     /gene="sitB"
                     /locus_tag="JNZ05_003939"
     CDS             complement(2022..2849)
                     /gene="sitB"
                     /locus_tag="JNZ05_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HAY8566940.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GNVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2849..3739)
                     /gene="sitA"
                     /locus_tag="JNZ05_003940"
     CDS             complement(2849..3739)
                     /gene="sitA"
                     /locus_tag="JNZ05_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HAY8566941.1"
                     /translation="MLLGCLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAE
                     VSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSS
                     GVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKA
                     KITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQV
                     RKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYID
                     LLKVTTSTLVQGIKAGKREK"
BASE COUNT         1101 a          995 c          990 g          975 t
ORIGIN      
        1 tgcaggcgca ggtcgatcgc aacgatgaga tttatcgccg tcagcgggag ctgaaggaag
       61 agctgaatag tctgaaggat ttgaattcgg ttaggaattt tatcgtggaa taacagaagc
      121 tgcggcacgt cgtatgcagg aacgtgccgc ggttggctgg taaactttcg atagtgcgag
      181 tattgaatga tttctaggcg ttatcgattt tacgtatttt ttgcatgata gaattcgtac
      241 ctcctcccaa cgaccttcca tggctttccg gacttctgta ggctggcgga cgatgctgtc
      301 ctcggatgtt gtgctaattg ggtgataaca gatcctgtgt atgggcgttt ccctttgtgc
      361 gtgcgttgat gctggtaacg gtaaacgtca tgatgaacac gaccgcgaat aagacgacaa
      421 tcgtcggagc tggtgcgctg tcgataaaaa aggacagcca gacaccagtc attgacacta
      481 ttaccgatat gccagtcgcc agtaggagcg caatatgaaa gcgttgtgtc aggagtactg
      541 caatggcgcc aggcgcaatg agcagagaaa tagagagaat gatacctact gctttcagcg
      601 tcgccacaat ggtcagggaa accatgcaca gcagcccata atgcagccat ctcgtatgca
      661 aaccgcttac ctgcgcctgt tgataatcaa agctgaacag caaaaaatcc cgccatttta
      721 cgctaataac aagtgtaacc agcccggcaa taatcaccgt ctggattata tcgccgatgg
      781 ttatcccgag catgtcgccg aaaagaatat ggtcaagatg tacgtctggt ttaattgcaa
      841 tatacaagat cagacctgcg gcaaacatgc cagaaaaaac aatccccata actgtatctt
      901 gcttgattcg gctgttgtct ttcaggtatc ccgtcgccac cgcgcagaat actccggcga
      961 cgaatgcccc tgtagccaga ggtaacccca gaatccaggt aagcacgatg ccaggaaaaa
     1021 ccgcatgact catcgcgtca cccatcagtg cccaaccttt caggaccaga aagaccgaca
     1081 gcagcgcaca gggtatggaa actaccagtg aaatcagcag tgcgtgactc ataaaggtga
     1141 actgcagtgg ctccagtaat aatgccatca tgagcgatcc tccagcgctt tatgcgtacg
     1201 ccgacgattt gccaaaagtc catgggtggg cgcaaataca aatgctaaaa gaaacagcag
     1261 tgtctgcgcc accacgatga ttccacctgt cgcgccgtcc agaaagtaac tgacccacgc
     1321 gccgaggaaa ctggttacac tgccaatagt tacagcaatt atgagcaggc ggggaaaacg
     1381 atcggttaac aaccatgctg ttgcaccggg agtcaccacc agacagatca ccagaaaggc
     1441 accaaccgtt tgcagcgccg caacggtcga aaccgacagc agggtgaaga agaggatttt
     1501 taatctgccc ggatgtaatc caatagcacg ggcgtgattc tcgtcgaaaa aggtcaccat
     1561 aagatctttc catttgaaaa aaagcacgat gatcgagaga atgccaatga tagtgagttg
     1621 caggatatcc gccggatcga ttgccagaat attgcccagg acgatggtct gaatgttcac
     1681 ggatgtcggg ttcaatgaca ccataaacag ccccaggcca aaaaaggacg agaagatcag
     1741 gccgatgatg gcatcttctt ttaagcgggt gcgttggtta agaaacagca tgctgcccgc
     1801 tgccagtccg ccggaaaaga aggctcccag agaaaacgga agccccagca tatatgcccc
     1861 cgcaacaccc gggacaatag agtgcgacag cgcatcgcca atcagcgacc agccttttag
     1921 catcagatag catgacagga aagcgcagag accgccgacc attgccgaga cccacatcgc
     1981 attgagcata tattcatagc tgaagggttc cagtagcacg ttcatcttgc ttccctctgc
     2041 accgctgacg gtcgatgtgc cacaaaaggg cgttcatcgt cagtaataat gctttcttcc
     2101 gagccgttaa gtgtgacatg gcgcagtacg ccgctaaaag ccagctccag gttctcggct
     2161 gtaaaagtgg tgtctgtcgg cccgcttgcc aggactgtgc ctttgaccat gaccgtataa
     2221 tcgcaaaatg tcgtgactga gccgagattg tgcgttgaga caagcatcgt tttgccttcc
     2281 gcgcgtaact ccctcaacag gctgatgatt ttcgcttcgg ttttcacatc aacgccggta
     2341 aaaggttcat ccagcaggat cacattaccc tgctgtgcaa tcgctctcgc cagaaagacg
     2401 cgtttctttt gtccaccgga aagctcgccg atttgacgat ggcgaaaatc caccatatcg
     2461 acacgttcca gcgcatcggt gacaatctga cgatccctct ttttggctat acgcaacata
     2521 cccatatgcc catagcggcc catcatcacc acatcttcca ccagtacagg aaatgaccag
     2581 tcaacctctt ctgactgagg aacgtaggca accaggtttt tctgtagcgc ctgtcgcgtg
     2641 ggaataccca gaacagatat ctttccgctg gtcagacgca caaatcccat aatcgcttta
     2701 aacaacgtcg attttccgga accgtttacc ccaaccagag cggcaataga tccacttggt
     2761 actgtgaagg atgcatcgcg cagtgctgtg tgcccgttac gccaggtgac agtaacatca
     2821 ttaacgacaa tgcctgcaga ttgcatcatt atttctccct tttcccggct ttaattccct
     2881 gtaccagcgt actggtagta accttaagaa ggtcgatata cgtaggtacc gggccgtttt
     2941 ctgtgctcag ggaatcgaca tagagcacac cgccgtagtg tgcgccggtt tcacgcgcaa
     3001 cctgacgcgc tggtttatcg gaaatcgtac tctcgctaaa gactgccggg atatgatttt
     3061 tcttaactat atcaacaacc ttacgtacct gctgcggtgt tccttgttga tcggcattaa
     3121 tcggccacag ataaagctct tttagcccca aatcgcgtgc gagataagaa aaagcccctt
     3181 cactggtgac catccatcgc tgattctcag ggagttccgt aatctgctta cgcagggggg
     3241 caagggtttg ggtaatcttg gctttataag tatcggcatt acgttggtag gtttgtgcat
     3301 ttgccggatc gtattttatc aacgcatcac gaatattatc gacgtaaatc agagcattat
     3361 ctggcgacat ccatgcatgg ggattaggtt tgccctcata gggtccttcg gtgatcccta
     3421 ctggcgtcac acccgaagag acaattactt ctggaacccc attgagatgc tggtaaaagc
     3481 gttggaacca caattccaga ttcataccat tggcgagaat cagttgtgcc ccctgcgcac
     3541 gtttaatatc gccaggggta ggctgatact catgaatttc tgcaccaggc ttggttatgg
     3601 atgagacttc tgcagcatct ccagccacgt ttttggccat atccgcgatg atggtaaatg
     3661 ttgtaatgac cttgaatttt tcagttgcac ttgcctgaaa tgcgatcgag caggtgaggg
     3721 cgaggcaccc caagagcatg gttacttttt ttattgagtg cataatttag tacctatagt
     3781 gagcaaaatt agaacaaaca tagcaacggc tatgttttat agcataacct atacaaaatg
     3841 aaaataatta tcattaacag ctttgaggcc taaaacaggg attaggttgt ttcctgctgg
     3901 cgacatgata ttgaggaaaa aagtagagca gctattaggc tttgaaatga ctttatgagg
     3961 aaaattgttg atggcaaaaa tggggcaaaa cgctgtataa ggggcaaaaa agagtgggtt
     4021 atcgtagctt attgttgtcg ctgataatgt ttaacatatt g
//
LOCUS       DADMEQ010000209         4010 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.211, whole genome shotgun sequence.
ACCESSION   DADMEQ010000209 DADMEQ010000000
VERSION     DADMEQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.211"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(247..1605)
                     /gene="plaP"
                     /locus_tag="JNZ05_003941"
     CDS             complement(247..1605)
                     /gene="plaP"
                     /locus_tag="JNZ05_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HAY8566942.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGSIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1595..1675)
                     /gene="yoeI"
                     /locus_tag="JNZ05_003942"
     CDS             complement(1595..1675)
                     /gene="yoeI"
                     /locus_tag="JNZ05_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HAY8566943.1"
                     /translation="MRNPKKWGKFFAYATVITVKENNHVA"
     gene            complement(1873..2802)
                     /locus_tag="JNZ05_003943"
     CDS             complement(1873..2802)
                     /locus_tag="JNZ05_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8566944.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2848..3672)
                     /locus_tag="JNZ05_003944"
     CDS             complement(2848..3672)
                     /locus_tag="JNZ05_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY8566945.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3755..4009)
                     /gene="yoeB"
                     /locus_tag="JNZ05_003945"
     CDS             complement(3755..4009)
                     /gene="yoeB"
                     /locus_tag="JNZ05_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HAY8566946.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1091 a         1039 c          935 g          945 t
ORIGIN      
        1 cgcccacgct gcatgacaaa tcccagcaga gcaccacaaa ttagcccgct taatatcatt
       61 gaaaacataa attaattaac cagatgaatg ttaatgggga aatttttcat gactggtgga
      121 atgcagacca ataaccaaat tctctaaatt agaacaaatg gttattaatg aggatttcga
      181 taaaaaagaa taaaaaaacc ggagccaaat gttcattcga ctccggttta ttattagaaa
      241 agatgattat gctacgtctt cgtactgcgg aaccggatta cggaagctct tggtgacgca
      301 agccaggtaa atcagaccga tagcagccca gatcagaccc agaaccattg agctttcttc
      361 caggttaacc cacagcgcac caacggtcag cgcaccacac atcggcagga acagatactg
      421 gaagtgatct ttcagcgtct tgttacgctt ctcacggatc cagaactgcg agatgaccga
      481 caggttaaca aaggtgaacg ccaccagcgc accaaagtta atcagcgccg ttgccattac
      541 caggtcgaag ttgattgcca gcagggcaat cgagccaacc aggatgatgt tcatcgccgg
      601 agtacgccat ttcgggtgta cataaccgaa gaagcttttc gggaacacgc cgtcacgacc
      661 cattacgtac atcagacgcg ctacgcctgc gtgcgccgcc ataccggacg ccagtacggt
      721 aatggtggag aagatcagcg cgccaacctg gaacgcttta cccgcaacgt acagcatgat
      781 ttcaggctgt gacgcatccg gatctttaaa gcgagagata tccgggaagt acagctgcag
      841 gaagtaagtt gcaaagatga agatcatgcc gccaatcagc gcggtcagga aaatcgcacg
      901 cgggatcacg cgctctgcat ctttggtttc ttccgacaga ttgctgatgc cgtcaaagcc
      961 ggtaaaggag aagcacagga ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc
     1021 agaccagaac ggacgcgtgc tcgccagcgt accagcgcct tcaccttcaa atacgccata
     1081 aacaaccatg cccagaatca ccgcgatcag cactacctgc aacacgacga ttacagtgtt
     1141 gaagttcgct acggatttca gactacgcag gttaaaggcg gtcataaagg ccaccagcgc
     1201 caccacaaac atccacgatg gaatggaagg caccagagct tcaaaataga ttttcgccag
     1261 cagaatgttg atcatcggcg cgaacagata gtcgagcaga gaagaccaac ccaccataaa
     1321 gccgacagtc gggctaatgg atttctgggc gtaagtgtat gcagagccag cagaaggata
     1381 gcggcgaacc agcttcccgt agctcagagc cgtaaacagg atcgcaatca atgcgaacgc
     1441 ataggctgtc ggcacatgac catccgtgag gcctgaaacg ataccaaatg tatcaaacag
     1501 cgtcatcggc tgcatatagg ctagacccat cattacaacc ggaactaacg taagcgtttt
     1561 acgcaattcc acgcgagagg tgtttggagt aacgttatgc gacatggtta ttctccttta
     1621 cggtgataac cgtcgcgtaa gcaaaaaatt tgccccattt ttttggattc ctcagcgaca
     1681 acaactgtcg atttttagta aatatctatc cggtacgaag cccggcctct tggtatgaat
     1741 gattggtttg aagcaaaaaa taaccgacgc taattaaacg tcggttttta gtcatttttt
     1801 gacagcggcg cattgtgcct aaatgggggg gaaatgacaa gagaatgaga ggcttgtcat
     1861 aataattttt ctttaaatgg ctgattttcc gtcatcagat tttgcataaa caccggcgat
     1921 cgcactattt gctaaaattt catctcgcca ccagccgctg gcaagccctg cggtctgttc
     1981 attccagcca atccagacag gttcaaagag agtttgggcg accactttct tctcgattaa
     2041 cgcgccacta tcgagaaaac gttgcgccag ataccgtggt aaatagccgc agccaaggcc
     2101 gctaatttgc agctccagtt tggttttaaa atcgaaaacg gtaatggctt cttgttcgtc
     2161 gagaagttgt gaggccgtag aagcgcctgc ctgcgcagtg tcccccacca caatcgcccg
     2221 atatcgttta atgatacgac ggtttaacgg ttcttcttcc tgggctaacg gatgatgagg
     2281 cgcgaccgca aagacttgtt caagatcgcc caaccgcgag aaaccaaact cactggaaga
     2341 tggtggctca tgcatcgcgc caacgataat atccgctcgc ccctgagtca gggcatccca
     2401 ggaaccaccg agtacgccat tgataaactt caggcgcgtc acgctatgat gttgataaaa
     2461 agcttcaatg agaggcgcaa gcagagaaaa aggaaacgtg tcgtccacgc caatcaccag
     2521 ctcgttttcc cagccttcat gcagtttgat cgcctgcttt tccagttctc gcacggtatg
     2581 cagaacttcc cgccctttct ctaataacat ttttccggtg cgggtgaatt tagcgcggtg
     2641 tccgctacga tcaagcaatt gaatatttag gtcgctttcc agcttgtgaa cggtgtaact
     2701 cagagcggac ggcgtcttgt agagtttggc tgacgccgcc gcaaaactgc cctctttttc
     2761 tatggcatcg aggatcatca acacatccag cagtggtttc atacacgccc ccttgcagtg
     2821 catgcggcgt cctgctggcg tgatcgttta ctccagcggc attaccagcg gatcgggata
     2881 ctggtattca aatcccagtt cattacaaat ccgactgcca tcaataatct tacctttgcc
     2941 gctgtccaga ctatttctga actgcggagg ttgcagcccc agtaaacggg ccatctgcgg
     3001 atagaaaaca ttacgcgcag ggtgagctgg cgcacatata ttatagatgt gtccgccttt
     3061 aggtgcctgt aacaacagag tgatagcgcc aataacatct tctaaatgga ctaaattaac
     3121 accatgttca ccatcagggg cggtttttcc ggcaaagaag cgtcccggat gacgtcccgg
     3181 tccgaccagg cccgcaagac gcagaatatc gaccgaagta ccgggtaaat tgtgcagcca
     3241 gtcttcgagt tcttctaata ctcgtccact gttggttacc ggattacgcg gggtagtttc
     3301 tttaaccgtg ccttgcgcgt cgccatagac agatgttgag ctggtaaaaa taattcgggg
     3361 aatacgatgg gccagcgcgc tatccactaa ctcttgtacc gcttgtaaat agaactcatc
     3421 gccggggccg ctacgacgtg ccggaagcgt aatgaccagc gcatcggcat ccataagggc
     3481 atccagatcg tcagaatcgc aaactaactc aggctccatg cgaagcagat agctatcaat
     3541 gccactcatt cgggccgctt cgacaccatc ttgtgtggtt ttactcccgg tgacttgcca
     3601 gcctcgcgct gaaagtgaca tcgccagcgg catacctaac caccctaacc cgacaattgc
     3661 gacctttttc atccgttatc tcctgataca ccttagatct ataaggctac gctagcgtat
     3721 caaaactgac aattcattct atgaatgaat ctgttcaata atgataacga catgctgcaa
     3781 tgagcagtga atcatcagta accgcgtata ccagacggtg ctcctctgta atgcgtcggg
     3841 accagaaacc tgacaaatta tgtttcaggg gttctggttt ccccttacct tcaaatggtg
     3901 ttctgcgggt atctttgata agttcattga tctttttaac aattcgctta tctgtttcct
     3961 gccagtacag ataatcatcc catgattcct cagaccagat tagtttcact
//
LOCUS       DADMEQ010000210         3977 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.212, whole genome shotgun sequence.
ACCESSION   DADMEQ010000210 DADMEQ010000000
VERSION     DADMEQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.212"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..92)
                     /locus_tag="JNZ05_003946"
     CDS             complement(<1..92)
                     /locus_tag="JNZ05_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HAY8566947.1"
                     /translation="MRAPRITQKEMTEREQRELKTLLDRARIAHG"
     gene            complement(331..1680)
                     /gene="lysC"
                     /locus_tag="JNZ05_003947"
     CDS             complement(331..1680)
                     /gene="lysC"
                     /locus_tag="JNZ05_003947"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HAY8566948.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            2205..3854
                     /gene="pgi"
                     /locus_tag="JNZ05_003948"
     CDS             2205..3854
                     /gene="pgi"
                     /locus_tag="JNZ05_003948"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAY8566949.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT         1008 a         1050 c         1004 g          915 t
ORIGIN      
        1 ccatgcgcaa tccgcgcccg atccagcagt gttttaagtt cacgctgctc gcgttcagtc
       61 atctcttttt gggtaatacg tggtgccctc agtgagccag agagttcagt gtacgggaat
      121 tcgtttgcga gcagaactga taatattcat tttgccttct cttaactatt tatctctttc
      181 gataattaat tagagatgca gataaaaaaa ttaaaggcaa ttattctccg ataaagacgt
      241 tggttaacag agtacaggct cgtttattga tgagcatagt gacaagaaaa tcaatacggc
      301 ccgaaatata gcttccaggc catacagtat ttactcaaac aaattactat gcagtttttg
      361 caccacctgc tcggcatctt cgccgggcac caggaagcac aggttatggc tggatgcgcc
      421 gtaacaaatc atgcgaatgt tgaacggttc cagtacgccg aatacctctt tgccaacgcc
      481 gcaggctttt gacaggtcat tgccaatcaa cgcgaccagc gccagacctt cttccacctc
      541 cacccggcac agtgcggaaa gctccatcag cagagattgc gtcagcaacg tatcgccagt
      601 ggaggttgaa ccggtggtat caagggttaa tgccacgctc acttctgacg tggtgattaa
      661 gtctaccgaa atattatgcc gcgcgaggat gccgaaaact tccgcgagga agccgcgaga
      721 atgcagcata ttcaggctgt gcaaagtgag cagagtctga ttgcgacgaa gcgccagcgc
      781 gcggaacagc ggcggatttt cagttttatt gcacaccagc gtaccacctg cgcgtgggtc
      841 tttgctggag ccgacaaaga ccgggatatc gctgcgtact gcgggtagca acgttgccgg
      901 atgcagtact tttgcaccaa aagttgccat ctctgccgct tctgcaaacg cgatttcatc
      961 aatgcgtttt gctgcggaaa ctacgcgtgg atcggtggtg tagatgcccg ggacgtcggt
     1021 ccagatatca acacgagatg cgtgtaaagc ctccgccagc aaggctgccg tataatcgct
     1081 gcctccacgg ccaagcgtcg ttgtacgacc tttattttcg ctaccgataa atccctgggt
     1141 gatcactaag ccttcattga ggcgtgggag cagctgcagc gcggccagtt ccgccagcgc
     1201 ggctacatct ggctctgcac gaccaaatcg gtcgttggta cgcatcactt tacgtacatc
     1261 aaaccactgt gcctgaacat cgcgttcgcg caggatttcg acaaacagca gggtcgacat
     1321 cagctcgccg tggctgacca gctcatctgt cagcgccgga gacgttgcca gcgccgccgc
     1381 ttctgccaga acagtaatgt tctccagcag acgttcaatc tcttcacgga taacgttcgg
     1441 gtaacgcaga cgttccagaa tggcaaactg gatgttgcga atagcgtcga gtttttcgaa
     1501 tcgctcgcca ggttccagcc cttccgctaa agcgaccagc agattagtga taccagcaga
     1561 agccgagagg actactaaac gcacgttggc atcagaaagc acaatatcag cgctgcggtt
     1621 catggcgtca aaatcagcta cgctggtacc gccaaatttg gagacaacaa tttcagacat
     1681 aactacctcg tgtcagggga tccattttca gccttggcac aagggaagag cggaagacgg
     1741 gtgggcgcag agcgatactt cgctactatt ttcacccaga agtgctccac cacttgcgaa
     1801 acgcccgact gcgaacgctt ctggtgacaa cccaggggat tcagcccctg tagccgatga
     1861 tgaacgtggc cagccgttca atcacctcgg cgatgcaccc cctcaggtgt tatcacagga
     1921 ctggctcctc caacaccgtt acttgggcaa cgcgcctctt ctggcctgcg ctagcgcagg
     1981 tagtacattt ataaataaag ggtgagcggg gcggttgtca acgacggggt catgcggatt
     2041 tttcatccac tcctggcggt cagtagttca gctaataaat gcttcactgc gctaagggtt
     2101 tacactcaac attacactaa cggcactaaa accatcacat ttttctgtga ctggcgctac
     2161 aatcttccaa agtcacaatt ctcaaaatca gaagagtatt gctaatgaaa aacatcaatc
     2221 caacgcagac cgctgcctgg caggcactac agaaacactt cgatgaaatg aaagacgtta
     2281 cgatcgccga tctttttgct aaagacggcg atcgtttttc taagttctcc gcaaccttcg
     2341 acgatcagat gctggtggat tactccaaaa accgcatcac tgaagagacg ctggcgaaat
     2401 tacaggatct ggcgaaagag tgcgatctgg cgggcgcgat taagtcgatg ttctctggcg
     2461 agaagatcaa ccgcactgaa aaccgcgccg tgctgcacgt agcgctgcgt aatcgtagca
     2521 ataccccgat tctggttgat ggcaaagatg ttatgccaga agtcaacgcg gtgctggaga
     2581 agatgaaaac cttctcggaa gcgattatct ccggtgagtg gaagggttat accggcaaag
     2641 caatcactga cgtagtgaac atcgggatcg gcggttctga cctcggcccg tacatggtga
     2701 ccgaagcact gcgtccgtac aaaaaccacc tgaacatgca ctttgtttct aacgtcgatg
     2761 ggactcacat cgcggaagtg ctgaaaaaag taaacccgga aaccacgctg ttcttggtag
     2821 catctaaaac cttcaccact caggaaacca tgaccaacgc ccatagcgcg cgtgactggt
     2881 tcctgaaagc ggcaggtgat gaaaaacacg ttgcgaaaca ttttgcggcg ctctcaacca
     2941 atgccaaagc cgttggcgag tttggtattg atactgccaa catgttcgag ttctgggact
     3001 gggtcggcgg ccgttactct ttgtggtcag cgattggcct gtcgattgtt ctctccatcg
     3061 gctttgataa cttcgttgaa ctgctttctg gcgcacacgc aatggacaag catttctcca
     3121 ccacgcctgc cgagaaaaac ctgcctgtcc tgctggcgct gattggcatc tggtacaaca
     3181 atttctttgg tgcggaaact gaagcgattc tgccgtatga ccagtatatg caccgtttcg
     3241 cggcgtactt ccagcagggc aatatggagt ccaacggtaa gtatgttgac cgtaacggta
     3301 acgttgtgga ttaccagact ggcccgatta tctggggtga accaggcact aacggtcagc
     3361 acgcgttcta ccagctgatc caccagggaa ccaaaatggt accgtgcgat ttcatcgccc
     3421 cggctatcac ccataacccg ctctctgatc accaccagaa actgctgtct aacttcttcg
     3481 cccagaccga agcgctggcg tttggtaaat cccgcgaagt ggttgagcag gaatatcgcg
     3541 atcagggtaa agatccggca acgcttgact acgtggtgcc gttcaaagtg ttcgaaggta
     3601 accgcccgac caactccatc ctgctgcgcg aaatcactcc gttcagcctg ggtgcgttga
     3661 ttgcgctgta tgagcacaaa atctttactc agggcgtgat cctgaacatc ttcaccttcg
     3721 accagtgggg cgtggagctg ggtaaacaac tggcaaaccg tattctgcca gagctgaaag
     3781 atgataaaga aatcagcagc cacgatagct cgaccaatgg tctgattaac cgctataaag
     3841 cgtggcgcgg ttaatcatca tgcgtgcctg atgccgatat gtaggccgga taaggcgttt
     3901 acgccgcatc cggcaaccgg agcctgatgc caatatcgac attaatgcat agtatgagga
     3961 gttaaataac cttaaaa
//
LOCUS       DADMEQ010000211         3670 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.213, whole genome shotgun sequence.
ACCESSION   DADMEQ010000211 DADMEQ010000000
VERSION     DADMEQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.213"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            107..418
                     /locus_tag="JNZ05_003949"
     CDS             107..418
                     /locus_tag="JNZ05_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005086658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAY8566950.1"
                     /translation="MDAHWKTVLKMSVKRWLWLIIVSVLMFATTGSLLWCQGMKINAN
                     MNILREQKESLEKLNAKTWGVRYNEDSNGRFLVLPEGMKAETNWTMENGKQNAVRLVQ
                     E"
     gene            425..658
                     /gene="mbeD"
                     /locus_tag="JNZ05_003950"
     CDS             425..658
                     /gene="mbeD"
                     /locus_tag="JNZ05_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HAY8566951.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(1237..2355)
                     /locus_tag="JNZ05_003951"
     CDS             complement(1237..2355)
                     /locus_tag="JNZ05_003951"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY8566952.1"
                     /translation="MNSEIKVMSNRDPSIDTLRVTACIFVIISHYGYIFSQTRLSFIP
                     SFYGGVLGQVGVSLFFFISGYLAFISLDRKNTIDYFLDKGVRILTPYIITYFFISLLL
                     ILLSYLNKEIINTVPLVSIMTTGGQYTRIIPVFFALDGILNAHFSYDFLFLTGEWFIG
                     CVIMLYVISPIVFHVMNTFTFKRNIFFLFVITITTFPISNISSSFLNFPFWFFACRLP
                     EFMVGMLAFKYKNKLQKNTTVILSVVICILTVLLIGPNEFKTNIVNHQLSIIPLMCIS
                     IIIYKAALIINKKFNLYFFNSYAKCIYISMLIQHVVIYRLTSIIEINNASVFGYLFIL
                     LMVFFLTFYLSKKILIPTSKIESILIKKMNIINAKKAN"
     gene            complement(2473..2562)
                     /locus_tag="JNZ05_003952"
     CDS             complement(2473..2562)
                     /locus_tag="JNZ05_003952"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566953.1"
                     /translation="MRQHLTRKGQKSLSLSTSVEQHDKAISTI"
     gene            complement(2998..3159)
                     /locus_tag="JNZ05_003953"
     CDS             complement(2998..3159)
                     /locus_tag="JNZ05_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY8566954.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSL
                     EVMRLLRLN"
BASE COUNT         1075 a          722 c          859 g         1014 t
ORIGIN      
        1 cagggaactg acctcaaacg aatctttaat caggaacgct atcaacgacc atacatcagc
       61 cctgaaagaa cttctggaga ggtatcagaa aacgaccgtc gatacgatgg acgcacactg
      121 gaaaaccgta ctgaaaatga gcgtgaaacg ctggctgtgg ctgattatcg tctcagtact
      181 gatgtttgcc acgaccggga gcctgctgtg gtgccagggc atgaagataa acgcgaacat
      241 gaatattctc cgcgagcaga aagagagtct ggagaagctg aacgcgaaga catggggcgt
      301 gcgttataac gaggacagca acggacgttt tctggtgttg cccgagggga tgaaagccga
      361 aacgaactgg acgatggaaa acgggaaaca gaatgccgta aggctggtac aggagtaaca
      421 gagcatgacg gagctggaaa gacatttgct gaacgcatta gagcaactgc aacaggacta
      481 tatgcagcgg ctgaacgaat gggagagcgc cttcgtggaa ttgcagaaaa tgttttcgct
      541 tacgcaacgg gacaacgcga tgctgaacga gcgggtcatg cagttgagtc agcaggtgca
      601 gcacttgagc gagcagacag aacgcttgag ccagttatac agcgagaaca ggagataagg
      661 gacgaacggc tgatacagga atgcgaacat gcgttatccc tggagcgtga gcgacagccg
      721 gaaatacagg aacggacact ggatggccct tcgctgggat ggtaatcaga gacaggctaa
      781 aatgttgtca gcgcgtttcc ggtgcatttt ttgggataat ggttggtttg ggggcaccgg
      841 gaacgcgctt cagtcagacc ttgctgaaaa cataggaacg cgttaaaacg catacgggcg
      901 cgattctggt gtgtcttttg ttgctgtgtt ttgcctctat tgccagatcg cgctcattcc
      961 cctgaacttc tgttttcccg ctcaggtccc cgtcttcttc gcttccgaaa gcatgaaaaa
     1021 aatccgtttc agctgtctct ggctggcaat tcctgcgtga ttcacatggc tgcatagcta
     1081 tgcatgtggt taaaatttac cgggtgcgat cgcggcagtt tttcgggtgg tttgttgcca
     1141 tttttacctg tctgctgccg tgatcgcgcc gaacgcgttt cagcggtgcg tacaatgaga
     1201 tgttatggta aattcaagtc ttagatttta tgatgtttaa ttagcttttt ttgcgtttat
     1261 aatgttcatc ttttttatta aaattgattc aatctttgat gtgggtatta atattttctt
     1321 tgacaaatag aaagttaaaa aaaacaccat taatagtata aataaatacc caaaaacaga
     1381 agcgttatta atttcaatta ttgatgttaa tctgtatatt actacatgct gaatcaacat
     1441 ggatatgtag atgcatttag catacgagtt gaaaaaatag aggttaaatt tcttattaat
     1501 tattaatgct gctttgtaaa tgataatgct tatgcacatt aaaggtatta ttgataactg
     1561 gtgattgact atgtttgttt tgaactcatt aggccctatt aataatactg tcaatataca
     1621 tataacaacc gatagaataa ctgtggtgtt tttttgtaat ttatttttgt atttaaatgc
     1681 aagcatccca accataaact caggaagtcg gcaagcaaaa aaccaaaatg ggaagtttaa
     1741 aaatgatgat gagatatttg atatcgggaa tgttgtaatt gttatcacaa ataaaaaaaa
     1801 tatgttacgt ttaaatgtaa aggtattcat aacgtgaaaa actatcggtg atatcacgta
     1861 cagcataata acgcacccaa taaaccactc gcctgtaagg aaaagaaaat cataagagaa
     1921 atgggcgttt aatataccat caagggcaaa aaatactgga attattctag tgtactggcc
     1981 tcctgttgtc attattgata cgagagggac tgtattaatt atctctttat ttaaataaga
     2041 caataaaatt aagagtaaag agataaaaaa gtaagtgata atataaggag taaggatcct
     2101 tactcctttg tcaaggaaat aatcaatggt gttctttcta tctagtgaaa taaatgccaa
     2161 atatccagat ataaaaaaaa acaagctcac acctacctgg ccaagaaccc ctccataaaa
     2221 tgatggtatg aaagatagcc tggtttggct aaatatgtag ccataatgag atataataac
     2281 aaaaatgcag gctgttactc gcaatgtatc aattgacgga tctctattag acataacctt
     2341 tatttcgctg ttcaaattat cgcctttagt tgatgatgta aaattaacgc tataaataag
     2401 ctacacattc tagagcagcg aaaggatgat tcaagtattt ttaataataa aacgcattga
     2461 taatgtttat gttcagatgg tgctgatagc tttgtcatgc tgttccactg atgttgataa
     2521 cgacagagac ttttgtcctt ttcttgttag atgctgcctc agattcggat tatggcgtta
     2581 aaggggtttg acgctcaacc gaacggtttc acacgttaag agctttttgt ctgataaatc
     2641 aaaatataat ggacattgaa aaacgccctc aaaacggtgt ctttttaatc gaagattggc
     2701 acaaaacgga caccaccgtc cattagtcga ttttttagac ggcttttctc gcctaacgtg
     2761 agttttcgtt cggttgagca tcagacccct tttatattgt agatttctat ttttagacag
     2821 ttggaacagg acaattaaca acagatctac ttgagatcat ttgcttgtcg tcgtagtctc
     2881 tttcccagaa acgaaaaaac cgccctgcaa ggcggtttct cgaaggttct cagagcgacc
     2941 aactctttga accaaggtaa ctggcttatg aggagcgcag taaccaaatc tgtcctttca
     3001 gtttagcctt aacaggcgca taacttcaag actaactcct ctaaatccgt taccaatggc
     3061 taccgccact ggcgatttgt cgtgtctttc cgggttggac tcaagttgat agttaccgga
     3121 acgggcgtgg tagtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac
     3181 tgcctacccg gaactgagtg tcaggcgtgg aatgagataa acgcggccat aacagcggaa
     3241 tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagcca ccagggggaa
     3301 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcggtttt
     3361 tgtgatgctc gtcagggggg gcggagccta tggaaaaacg ccatcggcgc ggccttcttc
     3421 ctgttcctgg tcttttgctc acatgttctt tccggcgtta tcccctgatt ctgtggataa
     3481 ccgcatttcg tttttgagtg agtagatacc gctcgccgca gtcgaacgac cgagcgtagc
     3541 gagtcagtga gcgaggaagc ggaaaagcgc ctggacgtgc attttctcct tacgcatctg
     3601 tgccgcattt cacacccggt atggcgcact tttcatacaa tctgcactga tgccgcatgg
     3661 ttaagcccgt
//
LOCUS       DADMEQ010000212         3604 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.214, whole genome shotgun sequence.
ACCESSION   DADMEQ010000212 DADMEQ010000000
VERSION     DADMEQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.214"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..108
                     /locus_tag="JNZ05_003954"
     CDS             <1..108
                     /locus_tag="JNZ05_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="with Hns involved in transcriptional regulation of
                     hemolysin; non-specific DNA-binding protein which affects
                     the production of multiple proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8566955.1"
                     /translation="SERESFNSAADHRLAELITGKLYDRIPKEIWKYVR"
     gene            147..737
                     /locus_tag="JNZ05_003955"
     CDS             147..737
                     /locus_tag="JNZ05_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAY8566956.1"
                     /translation="MKLIIFILIVLIIATLLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            977..1237
                     /locus_tag="JNZ05_003956"
     CDS             977..1237
                     /locus_tag="JNZ05_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HAY8566957.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1461..1535
                     /locus_tag="JNZ05_003957"
     CDS             1461..1535
                     /locus_tag="JNZ05_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HAY8566958.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1528..2385
                     /gene="repA"
                     /locus_tag="JNZ05_003958"
     CDS             1528..2385
                     /gene="repA"
                     /locus_tag="JNZ05_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HAY8566959.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3085..3276
                     /locus_tag="JNZ05_003959"
     CDS             3085..3276
                     /locus_tag="JNZ05_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566960.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          957 a          909 c          900 g          838 t
ORIGIN      
        1 agtgaacgtg aatcatttaa ttcagcagca gaccaccggc tagcggaatt aatcaccgga
       61 aagttatatg acaggatacc aaaagagata tggaaatatg taaggtaata attcagcaat
      121 aattaattgc caggagcaga taaataatga agttgattat ttttattctc attgttctga
      181 ttattgcaac gctacttatc cggattatcc tcagaagtgt aaatcagcat tctcctttgc
      241 tgatgcaatt gcatgcggca ggtatacgga ctggtgatgc agagcgaata ctgtccagcg
      301 gtgaatactg gcaacgtcag aagacgctgc tgacagaaag ggaagtcagt tttatgaaag
      361 gactgttcag aattgtggat atgaagcggt ggtatctgtg tccgcaggta cgggtcgcgg
      421 atatcgtcca gctgaacggg aatatccggc cacgatcacg ccagtggtgg cagttattca
      481 ggatggtgtc tcagtggcat gttgatgtgg ttatcgttga gctgcgttcg ttcagtattg
      541 ttgcagcagt agagctggat gatgccagcc atttacgacc ggaacgcaga cgccgggata
      601 ttcttctgga agaggttctg aggcaggctg gtattccgtt gctcagaagc cacgatgcca
      661 gaaaactgct gcagatgacc ggagaatggc tgaatacaac aggggctgat cagcagtcac
      721 cggaacatcg tagctgacgc cttcgcgttg ctcagttgtc caaccccgga aacgggaaaa
      781 agcaagtttt cctcgctccc ggcgtttcca taactgaaaa ccatactatt tcacagttta
      841 aatcacatta aacgacagta atccccgttg atttgtgcgc caacacagat cttcgccaca
      901 attctcaagt cgctgatttc aaaaaactgt agtatcctct gcgaaacgat ccctgtttga
      961 gtattgagga ggcgagatgt cgcagacaga aaatgcagtg acttcctcat tgagtcaaaa
     1021 gcggtttgtg cgcagaggta agcctatgac tgactctgag aaacaaatgg ccgctgttgc
     1081 aagaaaacgt cttacacaca aagagataaa agtttttgtc aaaaatcctc tgaaagatct
     1141 catggttgag tactgcgaga gagaggggat aacacaggct cagttcgttg agaaaatcat
     1201 caaagatgaa ctgcagagac tggatatact aaagtaaaga ctttactttg tggcgtagca
     1261 tgctagatta ctgatcgttt aaggaatttt atggctggcc acgccataag gtggcaggga
     1321 actggttctg atgtggatgt acaggagcca gaaaagcaaa aaccccgata atcttcttta
     1381 actttggcga gtcagaaaga ttaccggggc ccacttaaac cgtatagcca acaatcaagc
     1441 tatgcgggga gtatagttat atgcccggaa aagttcaaga cttctttctg tgctcgctcc
     1501 ttctgcgcat tgtaagtgca ggatggtgtg actgatcttc aacaaactta ttaccgccag
     1561 gtaaagaacc cgaatccggt gttcacaccc cgtgaaggtg cccggacgct tccgttctgc
     1621 gggaaactga tggaaaaggc ggtgggcttc acctcccgtt ttgatttcgc cattcatgtg
     1681 gcacatgccc gttcgcttgg tctgcgtcgg cgcatgccac cggtactacg tcgccgggcc
     1741 attgatgcgc tgctgcaggg gctgtgtttt cactatgacc cactggccaa ccgcgtccag
     1801 tgttccatca ccacgctggc cattgagtgc ggactggcga cggagtctgc tgccggaaaa
     1861 ctctccatca cccgggccac ccgggccctg acgttccttg cagagctggg actgattacc
     1921 taccagacgg aatatgaccc gcttatcggg tgctacattc cgaccgatat cacgttcaca
     1981 tctgcactgt ttgctgccct cgatgtatca gaggaggcag tggccgccgc gcgccgcagt
     2041 cgtgtggaat gggaaaacag acagcgcaaa aagcaggggc tggataccct gggtatggat
     2101 gaactgatgg cgaaagcctg gcgttttgtt cgtgagcgtt tccgcagtta ccagacagag
     2161 cttaagtccc gtggaatgaa gcgtgcccgt gcgcgtcgtg atgcggacag gcagcgtcag
     2221 gatattgtca ccctggtgaa acggcaactg acgcgtgaaa tctcggaagg gcgcttcact
     2281 gccagtcgtg aggcggtaaa acgtgaagtg gagcgtcgtg tgaaggagcg catgattctg
     2341 tcacgtaacc gtaattacag ccggctggcc acagcttccc cctgaaagtg acctcctcag
     2401 aataatccgg cacgcgccgg aggcattcgc acgcctgaag cccgccagtg cacaaaaaat
     2461 cagcgctaca tacaaaaaac aacctcatca tccaccttct ggtgcatccg gtcccccctg
     2521 tttttgatac aaaacacgcc ccacagacgg ggaattttgc ttatccacat taaactgcaa
     2581 gggacttcac cgtaaggtta caaccgttca cgtcataaag cgccagccgt caaccctaca
     2641 gggtgcaatg tatcttttaa acacctgttt atatctcctt taaactactt aattacattc
     2701 atttaaaaag aaaacctgtt cactgcctgt cctatggaca gacagatatg cacctcccac
     2761 cgcaagcggc aggccccaac cgaagccggt ttagttacaa catgcaaaca taaaccccta
     2821 aaaaacccaa gtccaacgca gaactgaaac cacaacgccc ctcctcataa ctgaaaagcg
     2881 ccgccgcccc gcccgaaggg cgggaacaga gtcgctttta attatgaatg ttgtaactac
     2941 atcatcatcg ctgtcagtct tctgcctgga cgtatcgagt acacgctcgt aagcggccct
     3001 cacggcccgc taacgcggag atacgccccg acttcgggta aaaccctcgt cgggaccact
     3061 ccgaccgcgc acagaagctc tatcatggct gaaaacgggt atggcttagc agggctgggg
     3121 atgggtaagg tgaaatctgt caatcagtac cggctgacgc cgggcttcgg cggttttact
     3181 ccggtatcac atgtaaccac agcgtgtcgc cttccatgcc gctggcgcgg catcagaata
     3241 atacaagcag catttaatgc atttgcaaaa gtgtgagtaa tattaagtgg tcgtttcagg
     3301 gatatcgtta agacttgagc gataggaatt aaaaccccaa aaagattaaa aaaacaccac
     3361 aaaacggatg tttcttcaac accacttttg ctacatatga acggaaccga cgattaaact
     3421 ggatggctct gattgattca gggtatgaat ggcggttttt tgctccgttt ccctcaaaat
     3481 ggacgcaact tcccctctgc ggctctcagc cgcaccaccg catccgggcc agcggctcat
     3541 gcatcaggac ctgctctgcc agacggtagc cccgcttcag ccccgtaaaa cgcatctgac
     3601 tccc
//
LOCUS       DADMEQ010000213         3587 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.215, whole genome shotgun sequence.
ACCESSION   DADMEQ010000213 DADMEQ010000000
VERSION     DADMEQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.215"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(623..2332)
                     /locus_tag="JNZ05_003960"
     CDS             complement(623..2332)
                     /locus_tag="JNZ05_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HAY8566961.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVANSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2764..3483)
                     /locus_tag="JNZ05_003961"
     CDS             complement(2764..3483)
                     /locus_tag="JNZ05_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HAY8566962.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQCKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT         1068 a          719 c          640 g         1160 t
ORIGIN      
        1 aagcttgata caactggtct ggtatataca gtttctttga gtgcggttac gtccacagag
       61 gtaacggtaa aagtaacccc ttgtcgggac cactccgacc gcgcacagag gcattttcat
      121 cgctgacagg ccgcattgcc tcgcagggct ggaggaggat cggtgatatc cggacgaccg
      181 taccggctta cgccgggctt cggcggtttt actcctctgc catgtgcaac aacggagtgc
      241 cgccttacat gccgctggcg cggcatatcc tggtaacatt tttgttgata ccaccttccg
      301 gccttatcct gaccagtggg ctttcctggc atcgatgata ccgatgaacg cgcatgatat
      361 tgagccgacg atattacggg caactggaaa tactcactct ctggatgtga cctttatcca
      421 tgaagaagat ttggccacac catggaaacc tgaacaatca tcagtttacg cccatgtaaa
      481 cccctacggg atatttgaac tcgatatgga aacaaggttg ccaatcgagg tggttgcctg
      541 aaagttggca gttacccggt cactacacct tgttggagtg aacatatcaa aaacaggtaa
      601 ttacacaatt taaattacag tctcataaaa aatgattaaa tctatttatt atttcaataa
      661 acgttttttt catcttcttt gaaaattgtt ctgctatagt atccggttcc agattgttct
      721 cttttattat tctacaatac tctaatattg tttctttgtc tctatttact acagatacaa
      781 atacaccagg aataccatct ttattagttg cggacagcat ttcgaccaat cgtcttgtgg
      841 taaaactata gttttttaga atattacaaa aggcacgtat tgtttcggta tggccattat
      901 aaaatgccat aaaaagtcct gatgtaccgt tgggattttt tatccataat aattcatcta
      961 tatattcctg gtgagggggg agtattttta gtatatctcc atacgccata atggcctttg
     1021 tgtgcccatt ttgaagtgcc ataaacaacc catgctttac tttcaataat tctttaattt
     1081 gttcactact aagcattttc ttattaagca gacttccgaa catacgaata ctatctgcat
     1141 gtccattctg caatgcaaaa aataacccag gtaaatcatg gctgtctttc gcagctaata
     1201 gatataatct cttatcggaa gttaacgggg ttttaaagac tatttcccca taggctttta
     1261 ttgtgtctac atgtccgttc ataaaagaca tgcataaacc cggcacattg tttgccccta
     1321 ccgcagcaag caatgtaagt attgtttctt gactaagatt gcacatattt aatatcttac
     1381 catatacttg aataatgtct gaatatccgt tttgaagtgc tatataaaat gcaggagtac
     1441 catcagagct tctcgtggcc agaaggttca ccagtttaga ttgagaaatt tcaccatttt
     1501 tgtatttttc catcagtata aataatactt ctgtcagatt ctttgttatc gcatgatata
     1561 taactctgga attgatcaat tcatctggta aaagtttcac tgtgtttgtt agaggggaat
     1621 tatgcctatc ttcaaatatt acctgcccgg gttgctccag accataaatt ttccaataaa
     1681 aatcatcaaa aaaatcctta gcggataatt ggctcagttc gaaatggcta tcacaagtaa
     1741 atacagtacg tttatgagta acagttcgat taggatcgaa aaactgaaca acccattttt
     1801 cactttcttg tacgcgcttt attttcaacc ctaacgccat ttggtggttt aatgtgcatg
     1861 ctgataatgt tctgacaaag tctccatttt ttttcatgtt ataaaaaaaa ttagtaataa
     1921 ctgttcccca ctttttattt tccacaaaat aaatataagc tggtgatttt gaaaaatctt
     1981 ccgttttttc caaacataca ttttttgcaa tagcatcagg ggtgctaaga agctgatagt
     2041 ttaccttccc atttgtttcc ataaaattca tttcccaata ggaagctagg tgacggcaaa
     2101 ctatttgtct tccatatata tcttttgcca ctccatttat atttaccttc acatttctct
     2161 tttctgaaaa ataaggtgct gttttattct taaactgaat tagttcactg gagtttgcga
     2221 ccctcataat agaacttgtt attgttctat tatctctgtt aacagacata tctgttgata
     2281 acgaattttt tgttgaaaat atactagatg aaaatgtttt atttaacggc atagaagaca
     2341 accatagaga tgttataata ctattagatt gcagttacta atagtaaaag cctccttaca
     2401 aggaggtagc ttattcagaa tgctcctata agatagcgag ctcgtcaacg atacagatga
     2461 tctgattata ttgtgcaaaa ggagaacagg aaaataggca ctggaaaaga tgaaggcctg
     2521 atgggagcat taactaatgc aaaatccgac aggttaatat cccgaaaagc aatcaaacga
     2581 ctcaaacagg agatgcacgc tgaagtggtg gacaactgtt cggatttcag atgagtagct
     2641 cagcagttta tgagccgaat gctctgtcac gggcaggaat caggctgcat acgcagtgcc
     2701 aaatctaatc gtagttattc taaccagttt tcaagctaca aggtggtgta gctggcatct
     2761 tctctactct atcatcaaac gataaaatgg ttcctctcgt aacatttgct cttgcctttc
     2821 acttccatct cgatcgcttc gtagttcatt acgatagctg acatatttcc agtcttcagg
     2881 tctaacatct gacgccggat actccccggg agcgattttg cttcttaata gattagactc
     2941 gagacacatt ataaattgtc gtattttatg aagtaataaa gcactatatt gcgagcattc
     3001 ctgatctgat tttacatata gcgtaaactg agctcctatc cccacacgag attgctgaga
     3061 aactcgattc atatcagtta tcttccattt atctatagga ctgtcctctg aaaataataa
     3121 accagacaaa atttggaatg ccagaggaac ttgctcccta gctatactaa tatgaaactt
     3181 gtccccaaca aaatccccct tacactgagg attttcacga catgcatgaa taaacacatc
     3241 tccactatgg ttcacaatct ggaatccttg gtacatgaca gaaaaaaatt gacatatatc
     3301 agagtaagtt ggcacgttcg atgctacttt gaaatagtac gctgggtatt gccgtatttg
     3361 gttgtacaat atattgaagt tatttttcaa tctttcattc gcagaaagca tctgcggtat
     3421 ttcagacctc acaacattca aagaatctaa atttagtttt agacagggct tttttatggg
     3481 catagaaaac gtcctctata aaatagatat attatcttat attgattgtt tatcatcttt
     3541 tataaaacga tacattatat gtaatgtatg taagcgtaag ggtcttc
//
LOCUS       DADMEQ010000214         3541 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.216, whole genome shotgun sequence.
ACCESSION   DADMEQ010000214 DADMEQ010000000
VERSION     DADMEQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.216"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..221
                     /locus_tag="JNZ05_003962"
     CDS             <1..221
                     /locus_tag="JNZ05_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566963.1"
                     /translation="ANFIALQINGSKTGFAASITGFTQTISAGTISFLSVTVSDNGVG
                     PGTTLGLGSLITSLLSIAVIAIAGNNKK"
     gene            complement(862..1134)
                     /locus_tag="JNZ05_003963"
     CDS             complement(862..1134)
                     /locus_tag="JNZ05_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566964.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASSSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWKAIYRSTSHYGKNVLLLQLA"
     gene            1223..1402
                     /locus_tag="JNZ05_003964"
     CDS             1223..1402
                     /locus_tag="JNZ05_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566965.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            1454..1648
                     /locus_tag="JNZ05_003965"
     CDS             1454..1648
                     /locus_tag="JNZ05_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566966.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(2447..2783)
                     /locus_tag="JNZ05_003966"
                     /pseudo
     CDS             complement(2447..2783)
                     /locus_tag="JNZ05_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1131 a          761 c          693 g          956 t
ORIGIN      
        1 tagccaattt tatcgcatta caaataaatg gttcaaaaac aggatttgcc gcatctatta
       61 caggttttac tcaaacaata agcgctggta caatcagttt cctgtcagta accgtaagcg
      121 acaacggtgt tggtcccggg actactttag gtcttggttc actaataact tcattactgt
      181 caatagccgt aatcgccatt gcaggaaata ataaaaagta agtgtactct gactgcgtat
      241 gtatctactt tgtgtaatgc aggtgtctat tgaaaataat tcttttcgtg ggcatctgtt
      301 gtataaaata aaaaattatc gtaagtactc tatctgcgat gtcttgtgaa aattatcctg
      361 tctggcaaca atcgcgtcca tctgtattaa tggacgcgaa cgatgaactc tcaaacaaca
      421 ctagatatca ccggctttcg cacttatttg ccagaatcat tacgtctcag attcgacgag
      481 caattatctg tccgggctat cgcacagcag ttaagactca gttattcccc cattcacaat
      541 ctctttcaac gaattaacgc ctcaggtatt gtctgaccat gacccgagtc cctttcattc
      601 gcccaaccga taccatactt tatgccaaca gaaagaatga atgaaccgcg cctaaaatca
      661 gcaaggacac atggcacaaa gtgcgacatg tctcacattt aaccccagtg ggggcaacaa
      721 gtataaaacc gttttttgat taagtggtcg aatatgaaaa ggggaaggtt tatgtgaaaa
      781 atacctgtgt tttctttgaa aacacacaac agtatcaggt tatttatgtt tatacagtgc
      841 gttccaaact gacctgaata attacgcaag ttgcaacaat aaaacatttt tgccgtagtg
      901 tgaggtactc cggtaaattg ctttccaggt gcagacaatg actaacgaag aactacaggc
      961 aatcgcatta tccatgcttg agcgccagcg cgaaaaagta caccagcaac gagagtcttt
     1021 gcttaaccca tctccattca ccaggccaaa acgattggag cttgaagcat tcttggacaa
     1081 atacccacgg cggcttaaat caggcaaaac cagaccacca ggctgatagc tcacagtttg
     1141 ttctccgatt caataaaacg gataagaacc atcagcgcgg tagcgtaagg cactacagcg
     1201 cattgcttta cgagtaataa caatggattt atcaccagaa gacgcaaagt ttttacgaga
     1261 actgcaacac cgtatgctca gagaagccat tatcgaacag caaacgggca tgagcgccat
     1321 agacccattg cgatacaaac gggcctcatc tcctttagtt gacgacaatt ccaatgcgga
     1381 taaaacacgt ccaccacact gattacattt ttcattagca cagtggtgtg atgcagcata
     1441 cgaggaaaac cggatgaatg agaacaaaat aaaaaggctg gagcaactgt tacaggcacg
     1501 ccaacgggag tttgccacta aatggggacg tgccgcgctg ccacatgaac gcctaaccta
     1561 ttttccactt gaagacctca ttaaaaataa tggcaaaaaa atcaggtctg ataagaaaca
     1621 cttcttagaa tgcaatgaag acaaataaca acaatctgca tattgccacc aaccacagat
     1681 gtggcatcgt tatcagcgca cttgattttc tttatatcca ctgacgagct tgccggggca
     1741 cctttgtttg ccggtcattc tggtatcatg cagatattta cggcgtacaa gcccactagc
     1801 atgacattgt aataaggcct gagcctaacg ctttaatgtg tgtcatacac tcagtggcgt
     1861 aatcgttgac aatatggtgc atgtaatcgt aagcgtccag cgaaatccat ctggtcataa
     1921 tctgaagcat ccgacaaagt ggtgtccacc aaataagtga tctgcgccca atatcctgag
     1981 ccggaacttt gacaaaggcc aacgaaaaat gggagcggat gttacagagt tctcggtaca
     2041 ggggaaaagt ggtacctgtc gccagttctc cgatctgttt aaccgggaaa ttatctccta
     2101 cagcttgtca gaaaggtcgg tgatggagca tcgttaatac gatgctatac gatgcattca
     2161 cagtgcccgg ccagaggatg ccccgtcgct gcatatggat cagggttggc aatatcgaat
     2221 cgcaggctat aggcaaagtt aaagccccat ggagtagcac aaaatatgcc gcacaaagga
     2281 aacggtctga ataacgcagt gatgaagaac ttcttcagca cactgctaaa acgcatagtg
     2341 atcgagcgct gattctggcg aacaactgaa ctaatacatc agaatctgca ttatgttaaa
     2401 taaatatgaa aagatggttt aaataccccg ttacttttga cttactctat acggtatcgc
     2461 atcgtttaat attcgcaccg gccagatttt tatttctatt agttgtcaca atactgaatg
     2521 cgtacgacca cagtattctg gctcctgtgt ggttatgctt taattctgcg ttccgggcag
     2581 ataagcagtt gcttgcagga atccttcttg tgttaatgtc agttcccctt tttaccagtg
     2641 ctgatttcca cattccgtcc aacagagctt atagcctttc cctggattat agcattgtcc
     2701 ggctgaagtt ctttttgaat aataatagaa gcactgctgg cagatccagt ccgtttttca
     2761 taacccactg tactgataac cataatctaa tcagtagaaa ttgagtcgaa aataagcact
     2821 actccataca ggataattag aggtcagttt gattattcac aattcatcat cagcattttc
     2881 tatttctgac gaaatcaaca tgaaaataac catatatgat aattattata ataacggctt
     2941 taattggaat acatatatta caacgtatta tatataattg gtattctggg aactatattc
     3001 tcaaaataca gtagaaacga ggtatgtttc tggtggaaag gacagtggga ttaaaaagta
     3061 agaattgata aaaaaaacgc cagcaacaca tagtgccggc ggagggaata ccccatggag
     3121 aaaatgtgat gcctagagca tcatgaatat ataattaaaa atagttagcg ttgtcaatac
     3181 tttcacaaaa ataattttcg tagtatagaa agatattttt atgcatgacc tacctgaatt
     3241 tgctccgggt agaggttata aataaaaatt gaatcacgac aaacacaata ttcatagtat
     3301 ggcgatgcct acgccagcaa gaatagcgcc aataatattg ggaatataat agacaccaga
     3361 cgcacaggca tctcactcct taacaaacaa taatcaggat atttactttt acccagctaa
     3421 ctgtttacac ccaaagtgca cacaattaac cattcaataa caaatggcaa tatccatagc
     3481 catacgactt taccttgtaa tgttcggtat ttctttataa ttattctggg aaatctatca
     3541 t
//
LOCUS       DADMEQ010000215         3517 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.217, whole genome shotgun sequence.
ACCESSION   DADMEQ010000215 DADMEQ010000000
VERSION     DADMEQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.217"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(63..1505)
                     /gene="cusS"
                     /locus_tag="JNZ05_003967"
     CDS             complement(63..1505)
                     /gene="cusS"
                     /locus_tag="JNZ05_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HAY8566967.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            complement(1495..2178)
                     /gene="cusR"
                     /locus_tag="JNZ05_003968"
     CDS             complement(1495..2178)
                     /gene="cusR"
                     /locus_tag="JNZ05_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HAY8566968.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2335..>3517
                     /gene="cusC"
                     /locus_tag="JNZ05_003969"
     CDS             2335..>3517
                     /gene="cusC"
                     /locus_tag="JNZ05_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HAY8566969.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEKAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQ"
BASE COUNT          863 a          911 c          921 g          822 t
ORIGIN      
        1 ggtatgacta ttgcggcacg ttatttttac actggttata aaagttgccg tttgctgaat
       61 aattaagcgg gtaatgtgat aacaaacctt gtcccccgcg catctgacgt taccgcaacc
      121 gtgcctttat gcgcgacaac aatcgatttc actatcgcca gcccaatacc gctaccttca
      181 ccttttcgct ggcgggaggg gtcaacgcga tagaaacggt caaacaatcg cggtaagtgc
      241 tcgggcgcaa tgggcgtacc ggggttttcg acggaaactt gcacctggtg atcgaccgtc
      301 tgacagcgca ctacaattgt ctctctggtt ggcgtataac gcagggcatt agaaagcagg
      361 ttgcttagcg cccgacgcag catcaacgga tcgcccgcaa cctgacgttt gtcgccaaca
      421 aaccgcaact ccacgccgcg atcttccgct agcgcctcga aaaaatcgaa cactttgccg
      481 acttcatccg ccaggttgag cattttcttt tcggggatta gctggttgtt atcggcctga
      541 gcgagaaaca gcatatcgct gaccattttc gccattcgcg tcagctcttc gagattagag
      601 tagagcacat cttccagctc cttctggctg cgcgactggc tgagggcgat ttccgtttgc
      661 gtaatgagat tagtaattgg tgtgcgaatt tcgtgggcga tatccgctga gaaattggac
      721 tggcgagtaa agacatcctc aatacgctcg atcatatggt tgaacgacag taccagctgt
      781 tccagctcaa tcggcacggt ctgcgggtca aggcgaacgt cgagatcttt cgaggtgata
      841 ttctggattt gacggctgac gctgcggatc ggcgcgtgac ctttatgtac cgccaacagc
      901 acgataaaga cgatcaggat gctgattacc gatgcggtca taataagttt attcatcaaa
      961 tcatttatgt aatgaagatg aaaatcgatc gacagcgcga tgtagagcgt ataaatcggt
     1021 ttgccgtcca ccaacgggcc aaccggcaag ttaatcatcc gccagttgct gtgttccata
     1081 tgcccgtgac cgtggcctgg catcataatc gtcgggccgg aaaggagata cacctcgcca
     1141 ccccgcgcgt ctttatcggg tatggcatca cgtgcaaact cgcggatatc aggcgcaccg
     1201 ggggagtgat acaccgtttt gccgtggcta tctgccaggg aaatcaacac gttggaataa
     1261 ccactgacga tatcttccag cgtcattaag cgtcgggctt gcgtttcgtc agggtgattt
     1321 agtacccgtt caagggtggc gctaatctct tttaaatcat taatgtcctg ctcggcaaaa
     1381 tgcactttta ctgagtggat catgatccat gcaaaggcga aaaacgccgc gatggtggcc
     1441 aggctgataa aaaaggtcag gcgggttgcc agcgaaaacg ggcgctgaaa tggcttactg
     1501 accatccggc acctcaagca tgtaacccac gccgcgcacg gtttgaatca gcttcggctc
     1561 aaagtcgttg tcgattttgc cgcgtagccg cttcaccgcc acatcaatgg cgttagtgtc
     1621 gctgtcgaaa ttcatgtccc atacctgcga ggcgataagc gagcggggca gcacttcgcc
     1681 ctgatggcgg agaaaaaact ccagcagagt aaattcttta ctggtcagag tgatgcgcgt
     1741 gccgctgcgg gtgactttgc ggctgacgag atcgaccatc agatcggcaa cctgaaactg
     1801 actttcgata atcaccgccg ccccgcgccg caggagagtg cgcacccgcg ccagcagttc
     1861 agcaaaagcg aacggtttca ccaggtagtc atctgccccc aactccagcc ccttgacgcg
     1921 atgttcaatg gtgccaagcg cggtaagcaa cagaatcggc atccctttat tggcggagcg
     1981 caacatgcgc acgatatccc agccgttcac gtctggcagc ataatatcga ggattatcag
     2041 gtcataatca ccggtcatcg ccagatggta gccattcagc ccgttgtcgg ccaaatcgac
     2101 cacaaaaccg gcttcggtta accctttggt caagtattct ccggttttct tttcatcttc
     2161 gacaatcaac agtttcatat ttcctccgca tgttgcccgg gcaattctag agtagcggga
     2221 tcagatggca atcgcttatt ggcaaaatga caattttgtc attttcctgt caccggaaaa
     2281 tcagagcctg gcgagtaaag ttggcgacat aaaatcacca gaaattatga gcctatgtct
     2341 ccttgtaaac ttctgccatt ttgtgtggcc cttgcgctaa ctggttgttc actggcaccg
     2401 gattatcagc gtccggtaat gcccgtaccg cagcagttct cactcagcca gaacggcctg
     2461 gttaacgcag cggataacta ccagaacgcg ggctggcgca ccttttttgt tgataatcag
     2521 gtgaagacgc tgattagcga ggcgctggtg aataaccggg atttgcgcat ggcgacgctg
     2581 aaagtgcagg aggcacgggc gcaatatcgt ctgaccgatg ccgaccgcta cccacagctc
     2641 aatggcgagg gcagcggcag ctggagcggc aatcttaaag gcgattcagc cacgacgcgg
     2701 gagttctcga ctggccttaa cgccagcttt gatctcgatt ttttcggtcg cttaaagaac
     2761 atgagcgaag ccgagcgcca aaattattta gccactgagg aagcccagcg cgcggtgcat
     2821 attttgctgg tttctaatgt cgcgcaaagc tatttcaatc agcagctggc gtatgcgcaa
     2881 ttgcaaatag ccgaagaaac gctgcgtaat tatcagcagt catatgcgtt tgtcgaaaaa
     2941 caactgttga ccggtagcag caatgttctg gcgctggaaa aggctcgcgg ggtgatagaa
     3001 agtacccgca gtgacatcgc taaacgtcag ggggaactgg cgcaggcgaa taatgcattg
     3061 caactgttat tgggaagcta cggcaagcta ccgcaagcgc agacagtaaa cagcggcagc
     3121 ctgcaaagcg ttaaattacc ggcgggcttg tcgtcgcaaa tcttattgca gcgcccggat
     3181 attatggaag ctgaacacgc gttaatggcg gctaatgcta atattggtgc tgcgcgtgcg
     3241 gcatttttcc cgtctatcag cctgaccagc ggaatatcaa ccgccagcag cgatctgtcg
     3301 tcattattta acgctagcag cgggatgtgg aattttattc ctaaaattga gatccccatt
     3361 tttaatgccg gacgcaacca ggccaatctg gatatcgccg aaattcgcca gcagcagtcg
     3421 gtggtgaatt atgaacagaa aatccagaac gcctttaaag aggtggcaga tgcgctggca
     3481 ttacgtcaaa gcctggacga tcaaatcagc gcccagc
//
LOCUS       DADMEQ010000216         3495 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.218, whole genome shotgun sequence.
ACCESSION   DADMEQ010000216 DADMEQ010000000
VERSION     DADMEQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.218"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..265)
                     /locus_tag="JNZ05_003970"
     CDS             complement(<1..265)
                     /locus_tag="JNZ05_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HAY8566970.1"
                     /translation="MNPSQHAEQFQSQLENYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLL"
     gene            complement(262..930)
                     /locus_tag="JNZ05_003971"
     CDS             complement(262..930)
                     /locus_tag="JNZ05_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY8566971.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(994..1632)
                     /locus_tag="JNZ05_003972"
     CDS             complement(994..1632)
                     /locus_tag="JNZ05_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HAY8566972.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     ATAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(1645..3495)
                     /locus_tag="JNZ05_003973"
                     /pseudo
     CDS             complement(1645..3495)
                     /locus_tag="JNZ05_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
BASE COUNT          867 a          956 c          780 g          892 t
ORIGIN      
        1 ccagcaacaa tacaaagagt aacgcattcg accagtgcca taatctgacc gctttgctgt
       61 aaagataaac cttctcacca tgatcggtgg ctgatttctt cacgccacga gcacgaagca
      121 aggcgtgcag ccccagcacc aaccacatcc caaccagcaa tactccggca atgatcaacc
      181 acaccggcca aaattcggga gtgaactgtg gcacatagtt ctctaactgg ctctgaaact
      241 gttcagcatg ttgcgacggg ttcatacatt ttccttttta atcaaatgtt gaccgaaccg
      301 gcgatacaga tgcggttttc cggcaccagg tagctgatat tgatagtatt tattatcctg
      361 caaccacgcc tgaatttctg ggctatcttc gcgaccaaag atcaatgcgt gttccggaca
      421 ggcgctgacg caaatgggcg gaaagccttt tgccagtcgg gactcagcgc agaaatcaca
      481 tttatctgcc actttagtga cagggttaag gtagcgaacc tggtacggac aagcgccaat
      541 gcaataactg cagccgatgc actgcgattt ttctacccgc acgatcccct gctcatcacg
      601 ccacgatgcc cccgtcgggc atacgtcgat gcacggagca tcttcgcagt gctgacatga
      661 ctggcgaaaa aagtgatact gcgtttcgtt gtcgttatcg gtgactggaa tgtgtgctat
      721 cgataaacgg cttccctgag cggggacgtg gttagtttta cggcatgcgc gggcgcaaat
      781 attacagcca ttgcaccgtg actcgtcatg aatcatggca taccgaactt ccttttcttg
      841 cctcgtgttc gctaacagag aagacgcaga accagtaaaa aagattactg ttcccatccc
      901 cagaacaaat ttgcgtcgag tgaaggacat ctgattatcc cctaatacag tggctgcaac
      961 tgtccaccct atccatgaca tctccctgaa atttcagggg ttctgaaact gctctacctc
     1021 gaaccattcg cataagccat aagccttgcc ataggtcata aatcgctcgc taaacaagta
     1081 caacaccgcc gtcgatgact ccacctgccg tatatcggca tattcaggtg caacatccat
     1141 cgcggcggtc gcggcttcaa tctgcgcttc ctggaagtaa tatggcgctt gcatcagcat
     1201 ggcgactttg taaggacgag gatacgtctg gcactcaaaa cgaacggcgt gtgcaatggc
     1261 gcgacaaatg tcctgctcca caacttgcaa agacatcgcc gcgtagtttt cactcatggc
     1321 ctgcgttgag taatagtaat catcctgact tcccgtcata caagcgatat ccgcgtagtc
     1381 tccgttctgc ttaatgcttt ccagcaaaac tgacagttcc tcttctgcaa ccgaataggg
     1441 tggcgagaga aacacggtgc gcgccaccag ctgcccagcc gcagaatgct ggcgaatgaa
     1501 ctcagccagg atttcagcag gttctggttc ggttaccgta tcaacggggt gacgcatccc
     1561 ctgcaaccat tgccgcttcg cctcgtcaac ttcgctgact ttcgctaacg gcaattcatc
     1621 ctgatggttc atcttaccca ttccttacgc aggcagtaga ttgtgtgctg ccagatcggc
     1681 ggcaatatct tccagcccca gacgctgcaa tgtttcgcgg gttggacaac caagctgtgg
     1741 gtcccagccc atttctttgt agaacatcgt gagcgaagca tgcatatcgt cacgatccat
     1801 tttgtcagta ccttcagtaa agaccgggat ctgcggatcc ttgtcgaaaa cccaggaaca
     1861 gataagatcg tgttcattac gcatatcttt ggtttgcatc ggttttaccg tgtaggcacg
     1921 atgcaacgta aagatacgct ctgcagctaa atctaatttt tcctgagtca tctcttcgcc
     1981 ggtgatcgct ttgaagaatt tggcttcaag cgccagatcg ccccggtaat tacggctttt
     2041 cagcggcgaa acggtcattg gccagaccca gttgcacagg gtgacggcgt tatgcaaaca
     2101 gacccgcaac agcgaccact ttgcatattt aatttttgcg tcattgattg gcgtgtagtt
     2161 tttggtttca tcgtaagcat cttcagaacc aaaaagttct ttcgccactt cacgttgcag
     2221 tttcaatggc aagccggaac caataaagtt gatatgggta tgcgtcatgc agtcacggtt
     2281 gaacatacag ttaacaatgg aaccgacctg cgctgacgct tcattggcat ggtgaaccgg
     2341 atagccaaac ggcgaccaga gtttattttt cgcgtagccc cagtactctt tacccaaatt
     2401 ccagcgttct gcgatggcat atgaaccata agccaggtga ctcagctcac ccacacgatg
     2461 cgccagacgg tagtaaaaat ctttaatgaa gttaacgtca cccgcttcca gttgatccca
     2521 gcgaatttct gcatactctt cagctggcag aacacgcttg aacacacctt tgctgtaaca
     2581 ataagtaaag tcgcgatgca actgcccgta gttacaccat aagccgtagt catcgaacaa
     2641 attcagacca accaggttac caatcacacg accatcgtct ttatcttcaa aatctttcgg
     2701 cccgttcggg aagatggtgg tatggacaaa gtttgctaca caagtgttac cacctgtgct
     2761 gggcacgcca aactctttca cccgaggaat attcatttgg gtcatacaac ggatcgggca
     2821 agagtggcag ccgctcattt ttactgtgta tttctctgcc gccggtccta agtcaaaaac
     2881 ggatttatag gtacgaaagc cgaccgtatt ctgattgcct ggcggaattt cacccgtttc
     2941 aatcggacca ccttcagccg cgccccaaaa cagcccttta cgtgctgtcc agcgtgactt
     3001 gggatctgaa tactctgccc acgattgtgg cgtacttggc acgacatggt tgttattcgc
     3061 accaataagt tcagtcatca tgtaatcatt gagacgcttc atctcctgac gatcggcaat
     3121 gttgacccct ttcgtccctt caaccgcaat cgctttcagg tttttcgaac ccattattgc
     3181 gccagttccc gcaccgccgc tgtggttacg gctattcaac atgccagaga gaggaataag
     3241 gttttcccca gcctgaccaa tagccgccac acaggtttcc cgactggtca atcgacaaat
     3301 ttcttccgtc gttgcgcgcg tcccttttcc ccataagaaa tcggcttttt ccaggctaac
     3361 tttgtcatct ttaatcttca gccataccgg tgatttcgcc ttcccttcga taataatgac
     3421 gtcgtatcca gcgaatttca tttgcgctgc aaaaaagcca cccatatggg catcgcgact
     3481 aaatttcctt tggta
//
LOCUS       DADMEQ010000217         3388 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.219, whole genome shotgun sequence.
ACCESSION   DADMEQ010000217 DADMEQ010000000
VERSION     DADMEQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.219"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(185..880)
                     /locus_tag="JNZ05_003974"
     CDS             complement(185..880)
                     /locus_tag="JNZ05_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HAY8566973.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(873..2300)
                     /locus_tag="JNZ05_003975"
     CDS             complement(873..2300)
                     /locus_tag="JNZ05_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HAY8566974.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2311..3030)
                     /locus_tag="JNZ05_003976"
     CDS             complement(2311..3030)
                     /locus_tag="JNZ05_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HAY8566975.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT          847 a          945 c          708 g          888 t
ORIGIN      
        1 ggaacccgtt ttcaggaatg gatgcctgtc tttttcactg taaatatcct ggcattacta
       61 atgcctggat atttacagaa gagcagcata accatgacgg aacggggtta agtcaggttg
      121 tccttaacgc tattttcaac ctcgtatgcc ttcttcaggt ttatgttcag atctcatatc
      181 tctctcagca atcctcaata atcagataca ccgctttcac cggaccatga accccgacga
      241 ctttgataag ctcaatatcc gccgttgagc tgggaccact gatgatgtta atacatgacg
      301 gcattcgttc accggcctgc gctttctgat gcaatttttc tgcgagttgc gctacacgcg
      361 gcaggattgt gcttttacgc aggataaaaa gagaagattc cgggagcagg ctcaatgaac
      421 gcccgcgctc ggcggcggaa aaaagaacca cgcctcccga ttcggttaaa ccatattcag
      481 catacacgac acccacttta gcctgctctg cctgcgagat attctcggca cctttcgccg
      541 gatcccaaac aacggcattg cattcctgct gcaaacgttc actaatcccc aattcctcca
      601 gcctcgtgtc accgctaatc acgaccgact gatctcccag ctctttacac agacgtattg
      661 cagcttctgc cgccttcgcc tcgctggtca gctcacagcg cgtcaacata acatcgctgg
      721 caaactgaat aaacgcgtca cagcgctgct gttggttaag ttgggtaagc cgctcgttag
      781 catagttgtt aagcggcgca tcttctgctt gcggttcaag tcgcagcggg cgacccagtg
      841 cctgagcaac gttattcaaa aattcgctcc gattatccat tctttttctc ctgcgcctga
      901 tgtttcttaa accaactacg gaaactctct ccgtcagctt caggaagatc gcgtgcttcc
      961 atccagtcgc taatcgcgcc aaatttgagt ggtgttttac cgccattgat aaaccagctt
     1021 gccgcatgag cgccggccat catcccgact ttccacaatc ctggatgact attggcataa
     1081 gcgaacattt ttatcgcccg ttgctctgct tttgcggtga tccctttttc agccatcacc
     1141 cgacgatgac gcaaaatcag ttttgacagc ggaatacgca ccggacacac gctgtcacaa
     1201 gcagtgcata aagagcaggc gtagggtaaa tctttaaaat ctttatagcc gccaagtagc
     1261 ggagaaatca ccgcaccaat tggccctgga taaatagagc catatccatg accgccaata
     1321 tggcgatatg ccggacaagt attcatacaa gccccgcagc gaatacagcg cagcacatcc
     1381 cgaaattcag aggccagcac ctcagaacgc ccgttatcga caataaccag atgaaactct
     1441 tcaggaccat caacgtgccc cgcttcgcgc ggccctgtca gccaggtgtt gtatcccgtc
     1501 aaacgtgcac caacggcact gcgcgccagc atggtgatca atacatctac ctcggcaaac
     1561 gtgggggcaa tacgctccat tcccatcact gcaatatgcg ttttaggcag cgtggtacac
     1621 attcgcgcat taccttcatt ggtcaccagg cataccgaac cggtctctgc caccgcgaaa
     1681 ttacagccgg taatacctat ttcagcactg aggaaatctt cacggatttt ttgccggatg
     1741 aataaggtca tcgcttcagg cgtttccggc ccctcatagc ccagacgttc gtgtagcact
     1801 cgacggatct gatggcgatc tttatgaatt gccgggacca caacatgaga tggcggatct
     1861 tgatccagct ggagaatata ttcacccaga tcggtttcaa tcacctgaat gccagcatcc
     1921 tgcaacacat gattgacacc aatctcttcg gtcaccatcg atttagattt caccaccttc
     1981 cgggcatttt tgcgttgggc aacctgtaaa atgtagcggg tagcgtcttc tttggttttt
     2041 gcaaaataga cgtgaccgcc gttttgcgtc actttttctg agagctggta cagataagcg
     2101 tcgagattac tcaaaacatg atcacgtatc tgggcggccc gatcgcgcca ctcctcccag
     2161 tgccccaatt catcgaccat tttttgccga tttgccccaa tacgctgctg cgcgtttgcc
     2221 accgctttgc gcatgatcgg atcttcaatt tgctgacgga tgcgtgtctt aaaatctgta
     2281 ttactggttt tgatcgacat ctttatatcc tcagcggctc atcaacactt cagcaatatg
     2341 catcactttg actttctgcc cttcccgttg taatcgccca ctgatgttca gcaggcaact
     2401 cacgtcagca ccaattaaat actcagggcg gacttccatc aggtgcacaa ccttttcttt
     2461 caccatctcg ccggatattt cggccatttt gaccgagaac gtgccgccaa atccgcagca
     2521 ggtatcctgt tcagcaaagg tcaacagctc cagtccacgc acatttttca gcagcgtaag
     2581 tggctcgtcc ttcactccca gcttacgggc caggctacaa gatgggtgat acacagctct
     2641 cccttgcaaa ctggcaccta catcgactac ccctaattta ttaacaataa aagaggtgag
     2701 atcctgcata cgcgcggcaa ccttttcggc acgtgatgcc cattcaggtt catccgccag
     2761 atacgtcggg taacttttta cggcataggt gcaagagcca gccggtgaaa taatgggatc
     2821 gtcgttatcc tccagtgcgg cgatcagatt tttcatccct ggaatcgctt ctttgatata
     2881 accgctattg atcgcaggct gaccgcagca tccctgtttc tccgggaaat ttacgcgaca
     2941 gccgagtttt tccagtagca gcacggagtc tcgtgccatt cttgatttca gggcgtcacc
     3001 aatacaggtg acaaagaaat tgacattcac aactaatact ccattacttc atgcccattt
     3061 atgcccgatg tttccccccc accgggggat caatcatcaa taaatatggc gttcttttta
     3121 aattggctag caacatacca cccctcattt gtatgacaaa tgataatctt tcatccaaac
     3181 ttcaaatgca ataactaatt ttatttcttt cggcatcgct cacattttat ggggaaatcg
     3241 acgatcattt ttggcataag aaatccattt aaaaatggct acatccctta tacctattca
     3301 gagctactta ttcaggggtt attgagatat acactgatgg tgtacaaaaa aatcaccgac
     3361 aataatcacg tatcaagtaa tagtcgga
//
LOCUS       DADMEQ010000218         3327 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.220, whole genome shotgun sequence.
ACCESSION   DADMEQ010000218 DADMEQ010000000
VERSION     DADMEQ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.220"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            1..410
                     /locus_tag="JNZ05_003977"
                     /pseudo
     CDS             1..410
                     /locus_tag="JNZ05_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847259.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            994..1710
                     /locus_tag="JNZ05_003978"
     CDS             994..1710
                     /locus_tag="JNZ05_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566976.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGVLFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVRKHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2262..2666
                     /locus_tag="JNZ05_003979"
     CDS             2262..2666
                     /locus_tag="JNZ05_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566977.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2729..3028
                     /locus_tag="JNZ05_003980"
     CDS             2729..3028
                     /locus_tag="JNZ05_003980"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8566978.1"
                     /translation="MTKTVSTSKKTRKQHSPEFCSEALKLAERIGVAAAARELSLYES
                     QLYAWRSKLQQQMTSSERESELAAENARLKRQLAEQAEELAILQKAATYFAKRLK"
     gene            3025..>3327
                     /locus_tag="JNZ05_003981"
     CDS             3025..>3327
                     /locus_tag="JNZ05_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8566979.1"
                     /translation="MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTGISPRQQ
                     FRQHCDSVVLAAAFTRSKQRYGAPRLTDELRAQGYHFNVKTVAASLRRQGLRAKASR"
BASE COUNT          932 a          685 c          772 g          938 t
ORIGIN      
        1 gaagacccag atatgaacag gcacattcag agttacttta aagagcttac tgaacgtctg
       61 aggtgttaga gccatgactg ccgcagcatt gctggcggaa atgcctgaat cgagcagcct
      121 ctcgagacgt gaaatcagcg cacttgtcgg tgttgctcag gtaaaccggg actcgggaac
      181 tctgcgcggt cgacgaacca ttttcggtga ttgtgctggg gaagaacagc tctgtacatg
      241 gcggcgcttg aggcccccgg tttaatctgg tgataaaggc gttttacatg cgcctacttg
      301 cagccggaaa cgccaaaaaa gtggcactgg ttgcctgtat gcgtaagttg ctgaccatta
      361 tgaacgcaat gctcagaaaa aacgaagaat ggaatgaatc gtatttatga aaactggatt
      421 gtgggccata aatattccaa aaagatagcc cgctccgttt attaccggaa cgggcgagag
      481 catgacattg tctgttatta taatgagact gctgattcta cttattttta atttcaagaa
      541 aagcgttttc tacaaagaat tatgagattt aatgttttgt ttagacaata ttatcattct
      601 gttcaataat gaaagtcttc atcaacaaca agtaaaaaca acatccctct catcgaaccg
      661 gacttgcaga tttcccgcat ccggctctct tgcaacacat cagaccttca cgttcgggcg
      721 gttatgggga aggtccagca agctgtatga gccctaactg tttgaatacc agtagattac
      781 cagaaacttc tatcgattga agacattacc aactagcgtt actaaatgtt gaccaagttt
      841 tgttcacttg tctggttttg atagtagcat tctgcagagt attttgatat gataaatatg
      901 cattaacaat gatattattg ttaatttcct agcataaaaa ttaaaagatc aacttttatc
      961 tcgaatattt tttacattat cagttggttt cttatgaatg acaattcatt gcttagaaat
     1021 tcttcattat tcatcgccta tatgggatgt gtagggtggg tcagtgctta ttcttatggc
     1081 tggggaacct ctttttacta tggatttcca tggtgggttg ttggtgcagg tttggatgat
     1141 gttgccagaa gtttactcta tgctatcata gtaatgggta tactttttac cggttggggg
     1201 attggtgttt tattttttct tcttataaag aaaagaagta aaatacaaga cttgagtttt
     1261 tttagactat tttttgctat taccctactg tttttccctg taatattcga acttttaatt
     1321 ctcaaacaat atttcatact tccattatca ctttcatgca ttattagttc attggttatt
     1381 tcaataataa ttcgtatata tggacggatc ttttctgtat catgtttttc agatatccct
     1441 tttgttagaa aacaccgcat taagttaatt atggctggtt ttttggttta tttttggctt
     1501 ttttcctttc ttgttggctg gtataaacct caattaaaaa aagaatatca gatgctttgt
     1561 tataataata gttggtatta tgttcttgct cgttatgatt cacggctcgt tttgtctagt
     1621 tcatttaaag atgacagtaa cagattctta atatttaata cagaacagtc gggattttat
     1681 gaaatcaatg atgtttatgt cagaaagtaa taatcctctc ccacaaagtg aattaaatga
     1741 atatataatg cgttcgaagt atatgtattg agctgtcata ctgagaaaca aaccttaggg
     1801 cagcacaatt aaagccagaa acaactatgc aagaaagtta ctaaataagt gctcggaaca
     1861 gtaaaacact caggggggac tcagtccgga ttatgtaatc agataaattg ccaaacatca
     1921 caaatcacca gccggactga gtgatgccaa gcattgcacc aataccccgt gatgaacgat
     1981 gcctgatgca gaaagctccc tataaaacaa agaacaaaaa ctacgaccgg cgattcaccg
     2041 tcatactgat gctgcatcgt ggtgacactg tctgtcactt tcatgttgac tgaacaactc
     2101 tgctgcgaag aaggtgtacc gccggcggga tttgattgtc cggcgtctag cttccacttc
     2161 cctgatggaa ctgaaagttg ttatggccgg tcagcttgcc ctgtccgcag gacatgaggt
     2221 gctgtgacgg acagcaccag taacggaaag tgacaatgaa aatgaatact gcgctgatag
     2281 ttgcactaat gtgtatgtgg tatgcggtac ccgctgctgc aaaagagact ttactggcta
     2341 tgccacgtaa ttcaacagaa cattgctatg cggagattaa tgtgcacgga ccatatgggg
     2401 tgtatttccg agtggtcccg catccccccg ggggaaagag ctgggtcgaa tgtaacagtg
     2461 actactatta ctctgataaa ccaccgggag tacagatact cggaacccgt gcagggtgtc
     2521 gggtatacgg aatctgcggg accacctcga cactgcatgt ggctggaaga ggggttgtgt
     2581 gtattaaaaa tatatgcagt ccacgtggga tgataattca caggatacgg aaacgtcctg
     2641 tagtggctgt aagcgatgag atgtgatctt acccagcaat agtggacacg cggctaagtg
     2701 agtaaactct cagtcagagg tgactcacat gacaaaaaca gtatcaacca gcaaaaaaac
     2761 acgtaaacag cattcgcctg aattttgcag tgaagccctg aagcttgctg aacgcatcgg
     2821 tgttgctgcc gcagcccgtg aactcagcct gtatgaatct cagctctatg cgtggcgcag
     2881 caaactgcag caacagatga cctcgtctga gcgtgaaagt gaactggccg ctgaaaatgc
     2941 ccgcctgaag cggcaactgg cggagcaggc agaggaactg gctatcctcc aaaaggccgc
     3001 gacatacttt gcgaagcgcc tgaaatgaag tatgtcttta ttgaaaaaca tcaggctgag
     3061 ttcagcatca aggcaatgtg ccgcgtgctc cgggtggccc gcagcggctg gtatacgtgg
     3121 tgtcagcggc ggacaggaat aagccctcgt cagcagttcc gtcagcactg cgacagcgtt
     3181 gtcctcgctg cggcctttac ccggtcaaaa cagcgttacg gtgcaccacg cctgacggat
     3241 gaactgcgtg ctcagggtta ccactttaac gtaaaaaccg tggcggcaag cctgcgccgt
     3301 caggggctga gggcaaaggc ctcccgg
//
LOCUS       DADMEQ010000219         3148 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.221, whole genome shotgun sequence.
ACCESSION   DADMEQ010000219 DADMEQ010000000
VERSION     DADMEQ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.221"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            848..1012
                     /locus_tag="JNZ05_003982"
     CDS             848..1012
                     /locus_tag="JNZ05_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY8566980.1"
                     /translation="MARCIKSQSLTLLEKLDALDADEQAAMCKRLHELAEELQNSIQI
                     RFEAESETGT"
     gene            1199..1633
                     /locus_tag="JNZ05_003983"
                     /pseudo
     CDS             1199..1633
                     /locus_tag="JNZ05_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532398.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
     gene            1658..1792
                     /locus_tag="JNZ05_003984"
     CDS             1658..1792
                     /locus_tag="JNZ05_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY8566981.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(2037..3146)
                     /gene="fepE"
                     /locus_tag="JNZ05_003985"
     CDS             complement(2037..3146)
                     /gene="fepE"
                     /locus_tag="JNZ05_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAY8566982.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
BASE COUNT          934 a          780 c          652 g          782 t
ORIGIN      
        1 aaaaaaacac aatatattaa catatggttt ttttattcta tcatatgtta ggaaaaaacc
       61 taaaaaccat tcagcagaca tttaacataa cattttattg cgcgcaccgc taaaacgcgc
      121 tgaacgcgat tctgccgcaa acaccgcaaa aagagatgtt ttccccgggg taaaccggaa
      181 aaatgcgtca ggatcgcttt cagctcactg catagctatg catgaaagtg aatgacgatc
      241 ggtttgggcg catacagtgc ccatgagaca cattttcgga cattttcagt tcgtgaatcc
      301 ctgactcatt gctacatatc tgaacagaca cccctagcag cgcccccagg ggcgtcctgt
      361 aaaaaaacac accgcgccgc tagcagcccc ccccctaaaa agcggtgttg gcgcagctac
      421 aacaccgcgc cgacaccgct tttttaaata tcacaaagag agcaagaaga actaatttgt
      481 cataactctc tatttataaa gaaaaatcag caaaaacttg tttttgcgtg gggtgtgggc
      541 atttcaggca tgagaacaga ctcgcgtttt tccggagtat cagcggaatt attgattaag
      601 aaacactcga cgcagagcga aattttatgc aacagcggct gcactgcaca gggattattt
      661 atcagttgat atgctgtgcc atgatacgtc ctaaagaaac aggacaggat gtgcaattgt
      721 aataccgtcg cccgcaacgt cttacaatta ccacctgctc agggcccccc ccccccgaca
      781 cccggaactg acggagaaca acggaaggtg aacaaacaac aacaaacaac aacaaactgc
      841 gctgaatatg gcgcgatgta tcaaaagcca gagcctgaca ctgcttgaaa aactggatgc
      901 tctggatgcc gacgaacagg cggccatgtg caaacgactg cacgaactcg cagaagaact
      961 ccagaacagc atccagattc gctttgaggc cgaaagtgaa acaggaacat aacgaagctg
     1021 acggaggcgg tcacggcttt cccgtgggcg ttttcgtcag cagacaagcc cgtcagggcg
     1081 cgtcaggggg ttttagcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     1141 gagtatatac tggcttaatc atgcggcggc agagcagatg gtgagagaac agcgacatat
     1201 gaggtgtgaa atgctgcaca gatacgtaag gagaaaatgc agcttccggc gctcttccgc
     1261 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tgtctgctca
     1321 cgcaaagacg gatatacggt tatccacaga atcaggggat aacaccagaa aaaacatgtg
     1381 agcaaaaaat aggccccgaa aaaaggccgc gccggtggcg tttttccata ggctccgccc
     1441 ccctgacaag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1501 taaagatacc aggcgtttcc ccctggtggc tccctcgtgc gctctcctgt tcctgccttt
     1561 cggtttaccg gtgtcattcc gctgttatgg ccgcgttcgt ctcattccac gcctgacact
     1621 cagttccggg taggcagttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1681 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1741 aacgccattg gcagcggcca ctggtaaaca ggatgtgcga gagatgtatt gagagttctt
     1801 gaagtggcag ccttactgag gctacactag aaggacagat ttggtgacat gtctcgcaca
     1861 agactgttac cacggttcag aagttcccca acttactgaa ccttcgaaaa accacctccc
     1921 caggtggttt tttcgttttc tagagtagaa gactacggct gtaagggaat gatctcaaga
     1981 agatcctttg aactttttat aaaagttgct tataaaatga tgcatcagca caacttttac
     2041 tcaatcacac tcatctttct ggcctccatg gcatgacgta aaagtatata gccacatgca
     2101 agcatccctc caatgacaga agaaagaatt acaattatgg cattacttgg cccatccttt
     2161 ctgaccggta acgttgggct caattgatac tgaaatggca taaaatcaac atcccccact
     2221 ttcgccacaa ccaattgatt cacaacatat tgacgatttc gcaactcccc actcaattcc
     2281 gaaacatcct tgattgattt tttgatgttc aatttagttg ctataccatc agaaccgaga
     2341 gccacaggaa aatctgggtc atccttcata atctgaccat tgctgtatac aggttttttt
     2401 attccagccg cattagcaac ctccagtgaa taattgaggc gtcgaatatc tgcattcagc
     2461 tggtttctaa tttttatctc atccagtgca agaatttctt tttcaacatt agttttaact
     2521 tctagtttat ttcttatatc ttccatcaaa tcccttacaa caagggaaga aacataatta
     2581 atatacccag acaacacatc gtgagcttct ttacttgttg gagcagtaaa actcagagtc
     2641 catgatacat acaatgatga cttgtcgttt ttcttactgg cattatcatc aatcgacttc
     2701 atatttttac tcaactcaac aattgcacga tgcagttcga gagcgctaat gttattcttt
     2761 ttaataatct ccataacata aggagatgat ctcatatact cattcaacga attaacggac
     2821 tgaaatttct taataaacag agcaaatgcg ctatcacgat tgatatcaaa atccactccc
     2881 agaacatgaa gcttggaaag ctccttattt aattcactcc attgaacaga ctcagcagga
     2941 gtaaccacag cagaactagt ccatttttgt ggcaaaacaa aagaaacaag aaagccaata
     3001 caagcaaaaa atagaatcac cccaacaatt tttatttttg ctgcccataa aacgctaatc
     3061 aagccaaaaa ggtcaatttc ttcattacca ttcggcaaca tgttgtaatt agaaaaatgt
     3121 tcgtcagaga cctttgagat tttcattg
//
LOCUS       DADMEQ010000220         3142 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.222, whole genome shotgun sequence.
ACCESSION   DADMEQ010000220 DADMEQ010000000
VERSION     DADMEQ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.222"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="JNZ05_003986"
     CDS             complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="JNZ05_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HAY8566983.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGPRIYFSMSDGQ"
     gene            complement(1002..1097)
                     /locus_tag="JNZ05_003987"
                     /pseudo
     CDS             complement(1002..1097)
                     /locus_tag="JNZ05_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="JNZ05_003988"
     CDS             complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="JNZ05_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HAY8566984.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="JNZ05_003989"
     CDS             complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="JNZ05_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HAY8566985.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2194..3142)
                     /locus_tag="JNZ05_003990"
                     /pseudo
     CDS             complement(2194..3142)
                     /locus_tag="JNZ05_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          780 a          762 c          762 g          838 t
ORIGIN      
        1 tgttgtccgt cactcatgga gaagtaaatc cgtgggccgt ggtagtccgg cgaagaggtt
       61 aatctttgca gggcttgcag agcatgggat gataatgggt tatcactaat atgtattgaa
      121 cattcattcc tcagattaag aatgctttcc gggatatgac ttatctgatt tctatcaaaa
      181 aaatactctc tgacaacggg attatttccc tcagaaaatg ctggtagctc agtgaggaaa
      241 ttacgggtcg ctctgagatt tttaagagcc tgtgggagcg atggtaagga gactatttcg
      301 ttatagctga tattcatagt caataacgaa tcgggtaaag ctggcaggtt ttccagctca
      361 ttgtgttgta cctgaagtga ctccagcgca ggaggtagca cgggcaattc tgataattta
      421 ttgctggcgg aatataattt ttttagcgta acaggcagtt caggtaggtt agttaattga
      481 ttatatgata cattgagcag cgttatctga gctggtaagt tgtcaggtag cgaggacaga
      541 tttaaacggt ccagtcgaag ttcatcggaa ttattgataa gacattcttt aagtcgggag
      601 acagcctcat cacgctcttc accggggagc gcttgttttt cccatttatc ccatgctgaa
      661 aagtaatcag catatgtacc ggaaatagtg ttataaaaag aattttgggg caatgaaaag
      721 ttattattta tcggtaacat gatgggcatc cagttaaagc cggtggacaa atggctttgt
      781 taaaagaaat aagtaggagt ttttatgaag ttcattccat gaaatgaact tcagcacttt
      841 tttattataa taaggataaa caaaattggc tgtaaaaaaa cgatactgtt tcagttaacc
      901 gtgaatggcc tgtttgcacc gtattgttct tgtttttcgg ttagttaaac atcgtacttc
      961 atatttgaac ggtaagcggc tcgtcagaac cgtattgata tttactgaga ggtcagatca
     1021 actttccagg gcaacagatc gcgtacccgg tttgccggcc agtcctggat atgctcaatg
     1081 acgtaacgca gccattttgt caacgccacg atttttgacc ctagcctgac atttatattc
     1141 cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca
     1201 cttcttcccc gataacgaag atcggcacac tggccatatc agtggtcatc atgcgccagc
     1261 tttcatcccc gacatgcacc accgggtaaa gctcacggga gactttatct gacagcaggc
     1321 gggcactggc cagggggatc accatccgtc gtccgggcgt gtcaataatg tcactctgta
     1381 catccacaaa cagacgataa cgactctctc ttttataggc gtaaacctta aactgcattt
     1441 caccagtccc tattctcatc ggcaaaagag ccgttcattt caataaaacg ggcgatctca
     1501 gccatcccct gctgattttc agcctgccag cgttccgcac gcagacggcg ggcctctttt
     1561 tgcatggccg tatttaccag gcctgagata ttcacgtttg cggatttaag taactgatag
     1621 ctgtcactgt ctatagtgac agtaatacgc tgcttcatga taagccctca aaaacatact
     1681 tatgatatgt atatgagtat atattaatgg tacaaaccat caatatccat aaaaagtaca
     1741 ccattaattc tctataagtt tttaatacaa ttttttctgc tgcgattttt ttatactata
     1801 attagcatac ttataacacc gtaagtaaat tacatgaaag caggcacagc gtgatccgat
     1861 ttttctgcgt ataacgattt tccggaagtg taatacggtt gcgccccggg ccgggcaccc
     1921 ttttctgttg ttgtgtacgg gcccacagag aatggtaccg gaggcgggct cagaagtact
     1981 gagtcgggtg atcgcgtcag gccgcgaagt actgacacgg gtattggtga ctggtgatga
     2041 agtactgccc cgggtatgat ttcaggctga ttcaggttct gattctctgt cagtccgcgt
     2101 ggttgctggc ctggctgatt tcctgttgtc aggcgttcaa caaccgactg caggttatag
     2161 cccggttcac gaacacctga cggcaggtat cactcacccg gtgaaggtaa atccctcaag
     2221 aggcagcaac tctgccagtc gctcctccgg ccattccggc agacgcgtca ggacgtccgt
     2281 cagccaggca tgtggctcca gaccattacg ttttgctgtt tccagaaggc tcattatctg
     2341 tgccgcccgg tttccggcca tcagtgaacc ggcgaacagc cacgctttcc gtcccataac
     2401 aaccgggcga atggcacgct cgcagatatt gttgtccagg ggcaccgctc cgtcacccag
     2461 gaagcagctc agttcatcgc gccggttcag aatatagtta atcgccttat ttaatggccc
     2521 gtcgggcgga cagcacggct cctgctcttc aagccaggca aacaggtcat tcactatcgg
     2581 ccgggagtac cgttatcgcc actgccgtat tttttccaca gggcgctcac ggatgagttt
     2641 ctcaatgcgg taaagacccg cgatttttct cagctccacg cctgcacggg gctccctgct
     2701 ggccttatac agatttgcga agccgcgccc tgcgtgactc cagcaacagg ctgatgtgat
     2761 gccggggtga ttatccgcca gagatttgta cacgctgtat ccgtcactca ccagcgtacc
     2821 gccacaccag cactgcagcc aggtctcagg gtatctgagg gcccgtccgg tctgcgagtc
     2881 aaaacagacg accggcggac cgcttctttc accgctgacg taagcacaca gccagccgga
     2941 gcaggattta ccgcctttcc gggtatcaag gagcctcagg gacgtttcat ctgcatgaat
     3001 aacatcccgt gtcagaagct catggcgtag cagtttcgcc agcggggcca gtgcagctcc
     3061 ggcaacaccg accatatccg ccattgtact gacggggagc tccacaccca ttcgggagaa
     3121 aatgtgctgc tggcgataca gg
//
LOCUS       DADMEQ010000221         3058 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.223, whole genome shotgun sequence.
ACCESSION   DADMEQ010000221 DADMEQ010000000
VERSION     DADMEQ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.223"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            238..2106
                     /gene="kup"
                     /locus_tag="JNZ05_003991"
     CDS             238..2106
                     /gene="kup"
                     /locus_tag="JNZ05_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HAY8566986.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2273..2692
                     /gene="rbsD"
                     /locus_tag="JNZ05_003992"
     CDS             2273..2692
                     /gene="rbsD"
                     /locus_tag="JNZ05_003992"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAY8566987.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2700..>3058
                     /locus_tag="JNZ05_003993"
     CDS             2700..>3058
                     /locus_tag="JNZ05_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY8566988.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYV"
BASE COUNT          689 a          729 c          806 g          834 t
ORIGIN      
        1 gaagggcctt cccgagagtg gtaaaggaga ggccctggaa ataccccaca gccccaaaaa
       61 agagccagtc agttgcttaa ctgactggca ttactcaaag ggggggcttt ttctttgagc
      121 caggttaatg tggccgcatt taggagtacg attttgccgt taatcgtgca tactgtgcgc
      181 ttttttgtgg gccaagggac taagcacaca tttcatattt caacgaaaga ctagtctatg
      241 agcactgata ataagcaatc attgcccgcg attaccctcg cggcgattgg agttgtctac
      301 ggcgatattg gtaccagccc gttatataca cttcgtgaat gtttgtccgg ccagtttggt
      361 tttggcgttg aacgcgatgc cgtgtttggc tttttatcgc tgatcttctg gctgctaatc
      421 tttgtggttt ccattaaata tctcaccttc gtgatgcggg cagataacgc cggtgagggg
      481 gggatcctga cgttgatgtc gcttgccggg cgtaatacgt cggcgcgaac cacatcaatg
      541 ctggtgatta tggggctaat cggcggcagc tttttctatg gtgaagtcgt cataacaccc
      601 gctatttcgg tgatgtcagc cattgaaggt ctggaaatcg tcgccccgca gctggatacc
      661 tggatagttc ccctctcaat tatcgttctc actttattat ttatgattca aaagcatggc
      721 acggctatgg tcggtaagct gtttgcgccg atcatgctga cctggttttt gattctggca
      781 ggactggggt tacgtagcat tattgctaac ccggaagtgc tgcatgcgct gaatccgatg
      841 tgggcggtgc atttcttcct tgaatacaaa acggtttctt ttattgcatt aggggcagtg
      901 gtgctgtcga ttacgggggt cgaggcgctg tatgctgata tggggcactt tggtaagttc
      961 cctattcgcc tggcgtggtt caccgtcgta ttaccttcct tgacccttaa ttacttcggt
     1021 cagggagcgt tgttgttaaa gaacccggaa gcgattaaga acccgttctt cctgttggca
     1081 ccggactggg cgctgatccc actgctgatc atcgccgcac tggcgacggt aattgcctcg
     1141 caggcggtta tctctggcgt cttctcattg acgcgtcagg cggtacgtct gggatatttg
     1201 tcgccgatgc gcattattca cacctccgaa atggagtcag ggcaaatcta tattccgttt
     1261 gtgaactgga tgctctatgt cgcggtcgtg attgtgattg tcagctttga gcactccagc
     1321 aacctggcgg cggcgtacgg gattgcggtg accggaacca tggtactgac gtctattctc
     1381 tcgactaccg tggcacgtca gaactggcac tggaataagt attttgttgc gctgatcctg
     1441 attgctttcc tttgtgtcga tattccattg ttcaccgcta acctcgataa actgctctcc
     1501 ggcggctggt tgccattgag cctcggtact gtgatgttta tcgtgatgac cacctggaag
     1561 agcgagcgtt tccgcttgct gcggcggatg catgaacatg gtaactctct ggaagcgatg
     1621 attgcttcgc tggagaaatc accgcctgtt cgcgtgcccg ggaccgcggt gtatatgtcg
     1681 cgtgcaatca acgtcattcc ctttgcgctg atgcataacc ttaaacataa caaggtattg
     1741 catgagcggg tgattttgtt aactctgcgc accgaagacg cgccatatgt ccataacgtc
     1801 cgtcgggtac agattgaaca actgtcgccc actttctggc gcgtggtggc aagttatggt
     1861 tggcgagaaa cgccaaacgt ggaagaagtt ttccaccgct gcggtctgga aggattaagt
     1921 tgccggatga tggaaacctc cttctttatg tcgcatgagt cgttgatcct cggcaaacgc
     1981 ccgtggtatt tgcgtctgcg cggcaagctg tacttgctgc tgcaacgtaa tgcgctgcgt
     2041 gcaccagatc aatttgaaat cccgccaaac agggttatcg aactgggtac tcaggtcgaa
     2101 atctaacgcc agacgcctcc tttcttcata agggggcgtt tttgttttca tggttaatca
     2161 ccatgtaaaa cgtttcgagg ttgatcacat ttccgtaacg tcacgatggt tttcccaact
     2221 cagtcaggat taagctgcgg atcagcgaaa cgtttcgctg atggagaaaa aaatgaaaaa
     2281 aggcaccgtt cttaattctg atatttcatc ggtgatctcc cgtctgggac ataccgatac
     2341 gctggtggtg tgtgatgctg gtttacccat ccccaaaagt acaacgcgta tcgatatggc
     2401 attaacccaa ggtgtacctt cttttatgca ggtgctgggc gtcgtcacaa atgaaatgca
     2461 ggtcgaggct gccattatcg cggaagagat caaacaacat aatccgcaac tccacgaaac
     2521 gttgctcact caccttgagc agctgcaaaa acaccaggga aataccattg aaattcgtta
     2581 caccacgcat gaacaattca aacaacaaac cgcagaaagt caggcggtaa ttcgcagcgg
     2641 agaatgttct ccgtatgcga atatcattct ctgtgctggc gtgacgttct gaggccgtca
     2701 tggaagcatt acttcagctt aaaggcatcg ataaagcctt cccgggtgta aaagccctct
     2761 cgggcgcagc gttaaatgtc tatccgggcc gtgtgatggc gctggtgggc gaaaacggcg
     2821 cgggtaaatc caccatgatg aaagtgctta ctggtatcta tactcgcgat gccggcacgc
     2881 ttttatggct ggggaaagaa acgacattta ccgggccgaa atcttcccag gaagccggga
     2941 ttgggattat ccatcaggag ctgaacctga tcccgcagtt gaccattgcc gaaaacattt
     3001 tcctcggtcg tgagtttgtt aatcgctttg gcaaaattga ctggaaaacc atgtatgt
//
LOCUS       DADMEQ010000222         3050 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.224, whole genome shotgun sequence.
ACCESSION   DADMEQ010000222 DADMEQ010000000
VERSION     DADMEQ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.224"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(2..298)
                     /locus_tag="JNZ05_003994"
     CDS             complement(2..298)
                     /locus_tag="JNZ05_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HAY8566989.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            1050..1598
                     /locus_tag="JNZ05_003995"
     CDS             1050..1598
                     /locus_tag="JNZ05_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY8566990.1"
                     /translation="MAVIGYCRVSTQDQTVDNQKLQIEKVHKVDKWFIDEAVSGATKA
                     TSRDGFRNMMNYVRCDDTLVVVAIDRLGRNTVDVLSNVEALQTKGVRVVSLREGFDLL
                     TPIGRAMLTMMAGLADLEKDLISERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQG
                     KTRLQIQEELGISRATYYRLAK"
BASE COUNT          935 a          553 c          687 g          875 t
ORIGIN      
        1 ctcagaacac tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt
       61 acgggtcagc atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg
      121 ttgagccagt aaatcccgca gcccctggat cgttactgtc taccgctggc attggaccag
      181 ctgtcaacaa acgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa
      241 cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg
      301 gcacaactcc ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca
      361 acggaaagat gatgtctttg ttaaggtcat gcataaagta agggtaatta cgccaaaaat
      421 gacattttca ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa
      481 aatcgaggcc tggtttttct actgaaatga ttatgacttc aataacactt gaatgaagtc
      541 tattcggttt tctaggagac ctgttaaaaa caggacaata tcaggtgaat gactatgcaa
      601 tttaatagta gatttccaag tatctaagca gttttacaca aattttatgc agtatcggta
      661 tagtctcttt ttagcataac ttattgaaat atatagagaa tctgtgaagt ctcttttcag
      721 ggaactatgc agggtgttag tcgttcctct ctcgactcct agaaaagtgc tatatacagg
      781 gctatataac gatatataga tgtttggtca catggtgtaa ctatcaagaa acttaacgat
      841 ttcctacgtg aaagttagaa agtgatggtt atagttaccc tcctaaaatc ttcatagctc
      901 cttcataacc agtcttaaaa ggacttcctt ttgagacact ttcaacgtaa cggtagaatg
      961 caccctcaag tagtctcaaa aggttgtttt tggattatga gacagctaaa cttatgagac
     1021 taacttttga gacttctaag gggaacatta tggcggtgat tggttattgc agggtatcaa
     1081 cacaagacca gactgtagac aaccagaaac tacagataga gaaggttcac aaggtcgata
     1141 aatggtttat tgatgaggct gtctcaggag ctacaaaggc caccagcaga gatggtttta
     1201 gaaacatgat gaactatgta aggtgtgacg atacgttagt tgtggtagct attgaccgtt
     1261 tagggcgtaa taccgttgat gtgctctcta acgtggaggc actacagact aaaggtgtga
     1321 gggttgtttc tctacgtgaa ggttttgact tattaacacc aatcggtaga gcaatgctta
     1381 ctatgatggc tggtttagct gaccttgaaa aagaccttat cagtgaacgt agaaaggctg
     1441 gtattgaacg tgctaaagct gaaggggtac atatggggag gcctgtaaaa gcttcttcag
     1501 aggctgtaca gacgcttatt tcacaaggta agacgaggtt gcagattcag gaagaattag
     1561 gaatctctag agcaacttat tacagacttg ctaaataagt tactgaatag ttacatggat
     1621 gtagctgata tttggctttt ttgcaaaggg ggtcacatag ctggtgtaac aggtacttag
     1681 cagctacaga catctgtcag atatttttga agattctcag agagggaatt tgaagagggg
     1741 ctactatgat tttataaaaa ttttcagagt ctgaaaagtt actgtacaga ttttatacaa
     1801 actaaaaagg actcccgtag gtgattcagt aatgaattac ttcatgaaga gtccctttac
     1861 aagtcgattt ttaatcgtgg ctacgacaat ccacccatac cagacctaac cgtaccccac
     1921 cttaccaagc cataccctac cccgccagac tataaggaag cgtagagaac cgatgaaggt
     1981 gtctgcacag ttgcatctta aacaaccatc acagtgatgt caaatagttc ttttaagtag
     2041 gaggggttcc cctatggtca catatcccct ctctcccgct tagtgtccct tgatagtatc
     2101 tagagagtat gcctgtaatg ttagagagta tgcctgtaat gtttctgtat caggtcagct
     2161 ataagaggtc tggatattac cttgtaagat tcttggtagt gattatgcag agggcttgat
     2221 aagagtgttt agcgggagga agagaaccat gaaaccgaag gtaggttcta tcttaacagt
     2281 gactgtaaga gatacctgaa aaaatacttt aacagaacta ccaagaagag agggaacgat
     2341 aagaccagag gtagtgacct gttaaacagg ctgtttcaaa gtctcttaaa agtctgttaa
     2401 gagggctagg tgaacaatcc taccattttg tcaagtgttt tgtctttaac aaaaagtgct
     2461 aaacttaaca aaaagcacta aatgctaaag agacgattaa gaaatatact aagaggggcg
     2521 cttcgcttgg ggtcactatg ttctttctct cccgttaaga ggatttacag ggacatttca
     2581 aagccctgat aaggtaagcc cgatgataac agaaacagag ggcaaaacca cttatcaaca
     2641 ctgtgaacga ataacgtaaa gggctggtta agaaagtcgg tttagggatt ggtaacaata
     2701 ccaattagaa gggagcttcc cttcctgggg tgtcgctcta tgggtgaagt taacctttga
     2761 agttctctgt gaagaaactg aacagctatt atattggcta attacaaccc tttgaaagat
     2821 aagcattttt agaagttact gttaaggttg cagagttgtc tgtgaagaga cttgtcagtt
     2881 ctctatattg ggcggttaca actcattgaa aaataagaac ttttagaggt tactgtcaga
     2941 gaggtgaata cttgatagat acagtcttcc ggttctgtca ggctattttt taacagatag
     3001 cacaaaatgc taaagatgcc cctatgccat ctcgtacagg ggggttttga
//
LOCUS       DADMEQ010000223         3049 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.225, whole genome shotgun sequence.
ACCESSION   DADMEQ010000223 DADMEQ010000000
VERSION     DADMEQ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.225"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="JNZ05_003996"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="JNZ05_003996"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HAY8566991.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLLGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="JNZ05_003997"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="JNZ05_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HAY8566992.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..>3049)
                     /gene="bcsE"
                     /locus_tag="JNZ05_003998"
     CDS             complement(1963..>3049)
                     /gene="bcsE"
                     /locus_tag="JNZ05_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY8566993.1"
                     /translation="EEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQN
                     GIWTLVQSEEAEIQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAA
                     TVVFSLQQNAQIEPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVI
                     PWNAPLSRCLTMIESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMM
                     NNPLLPAHGKGVLVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRIND
                     LDTALNHIFPLPTGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVI
                     NAEHDGRHWRRIPEPMRLLDDAVERSS"
BASE COUNT          762 a          786 c          806 g          695 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aagcaaccag gtgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctca
//
LOCUS       DADMEQ010000224         3037 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.226, whole genome shotgun sequence.
ACCESSION   DADMEQ010000224 DADMEQ010000000
VERSION     DADMEQ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.226"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            52..1083
                     /gene="ynaI"
                     /locus_tag="JNZ05_003999"
     CDS             52..1083
                     /gene="ynaI"
                     /locus_tag="JNZ05_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HAY8566994.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRERKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(1134..2747)
                     /locus_tag="JNZ05_004000"
     CDS             complement(1134..2747)
                     /locus_tag="JNZ05_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY8566995.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT          790 a          705 c          672 g          870 t
ORIGIN      
        1 gccaggggtt tccggcatga cgacaattat ctggaggagc ctgataaaac aatgatcgct
       61 gaactgttta caaataatgc gcttaatctg gtcattattt tcggtagctg cgcagcattg
      121 attctgatga gcttttggtt tcgccgtgga aatcgtgaaa gaaaaggatt tttattccat
      181 gcggtgcaat ttttaatcta caccataatt atcagtgctg ttggtagcat cattaattat
      241 gttatagaaa actataaact caaatttatc accccaggcg ttatcgattt tatctgtacg
      301 tccctgattg cggttattct gacgattaag ctatttctgc tgattaatca gtttgaaaaa
      361 cagcagatta aaaaaggtcg cgatatcacc agtgcgcgga ttatgtcgcg cattatcaaa
      421 atcaccatta ttgtagtgct tgttctgctt tatggcgaac atttcggcat gagcctttct
      481 ggcttgctga cctttggtgg tattggtggt ctggctgtcg gtatggccgg taaagatatt
      541 ctgagtaatt tcttttccgg gatcatgctc tatttcgacc gtcctttcag tattggcgac
      601 tggatccgtt caccggacag aaatatcgaa ggtacagtag cggaaattgg ctggcgaatt
      661 accaaaatta cgacctttga taatcgtcca ttgtacgtac cgaactcgct gttttcgtcg
      721 atcagcgtag aaaacccagg acgaatgacc aaccgccgca ttaccacgac cattggttta
      781 cgttatgagg atgcggcaaa agtgggcgtt attgtcgaag ccgtacgtga gatgctgaaa
      841 aatcacccgg ccatcgacca gcgacaaacc ttactggttt atttcaacca gtttgctgac
      901 tcgtcattga atattatggt ttattgcttt accaaaacca cggtatgggc tgagtggctt
      961 gctgcacagc aagacgttta tttgaagatt atcgatattg tacagtcaca cggcgcggac
     1021 tttgccttcc ccagccagac gctgtatatg gataacatta caccgccgga acagggtcgc
     1081 taaatcagac atcactggcg ttatccacaa gggtaacgcc agttcccatc acatcaatgc
     1141 ttcacaatat acatagtccg actgtacgct acatcttcag ggttattaat gggataacct
     1201 ttcagccacg gtttgattaa tcgtccattg gtatattgat aaattggtgc aatcggtgct
     1261 tgctccatga ggattttttc tgccgcgttg taatcggcat tacgcgcttt aacggtattt
     1321 tccgtcgatg cctgggccag aactttgtcg tatgccggat tgttaaagcg tgaaatattt
     1381 cctgaatgca ttgacgttaa taatgtcagg aaagtggagg gttcattata atcccccacc
     1441 catgaggcgc ggataacatc aaaattgccg gtgttacggc tatcgatata ggttttccat
     1501 tcctgatttt gcaatttaac atctacgcca aggttctttt tccacatcga tgctacagca
     1561 atcgcaattt tttgatggtt ttctgaagtg ttatacaaaa gcgtcagctt cagcggtttt
     1621 tgcggaccat aaccagctgc gctcaacaaa gtttttgcct gcgcattcag ttcttcctga
     1681 ctcatttgtt caaacggcga aggttccggc gtaaatccgg cggtaacatc tggtataaaa
     1741 tgccacgctg gcttttcgcc cgtccccaac actttttcgg tcatcaggcg gcgatctatc
     1801 gtcatactta atgccaggcg aacgcgctga tctgccgtcg gccctttttg cgtgttaaag
     1861 gcataataat aggtcccgag ctgcggtggc gtataaacct gccccggaat atccttcaac
     1921 agcttctgat acatattttt cgggaaggat tcggtgatat caatatcccc cgcgaggtaa
     1981 cgcttagtgg ctgcggattc ctgattaatt ggcaggaagg tcactttttg cagcaccgtt
     2041 ttggcgttat cccaataatg ggtattcggt accacgacca gtttttcatt gactacgcgc
     2101 tctttaagaa cataagcgcc attgccgatc agatttccgg gtttcgtcca ctctttaccg
     2161 ctttctacgt tggctttttg caccgggaag aaggcaaagt tagcggttaa attcacaaac
     2221 cacggcaacg gtttatcaag ctgaattttc aaagtatggg catcaactgc ggtgacgcca
     2281 agctgatcag gcgtagcttt accatcaata atcgcctgtg cgttgttgat tcccgccagc
     2341 gcggcaaacc atgcaaatgg cgacaatgtt tttgggtcca ccagacgttg ccagctgtag
     2401 acaaaatctt gcgccgttac cggtgtgcca tccgcccatt ttgcgttatc gcgcagggta
     2461 aaagtccaga tacggttgtc attacttttc cattgagtcg cgacgccggg gacaatctcc
     2521 cctttttcgt tctgattcac cagaccttca aacagatcgc gaatgacctg aatctctggc
     2581 aggcccacgg ctttagcggg atccagcgac gcaggctcat ctttaatgtg gcgcaccagc
     2641 tcctgcttct ctgccagtac tgtgccgctc ggaacttctg cagcatacga aagagaaatg
     2701 ctgctgacca acagcgcaca acacgtaact gaaacagagt gcttcataag tttccctcta
     2761 accgacatta aaattgatgc gtaattattt gtttcgcctt aaagaaatgc aaacaacttc
     2821 catcaaaaaa gtgacaaagg aagcatcaaa tgttttaagt atcaatatat caaatcagat
     2881 ttagtgtaaa taatagacgc caaaatgcgg caaagcacat agaacaccga acactacgtt
     2941 cgggcttcgc acgttcaaac cagttttcgg tatcataatc agcatctgtc gccattgtac
     3001 gaagaataaa aaataaccaa aacgttgaaa ggaactg
//
LOCUS       DADMEQ010000225         3018 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.227, whole genome shotgun sequence.
ACCESSION   DADMEQ010000225 DADMEQ010000000
VERSION     DADMEQ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.227"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..213
                     /locus_tag="JNZ05_004001"
     CDS             <1..213
                     /locus_tag="JNZ05_004001"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566996.1"
                     /translation="CADLNIPFFVDARNKKLVFWFVRVDDEGYPEIARCMEREFATIP
                     AGINADGMYCPECGTVHWPDGVIPPF"
     gene            261..690
                     /locus_tag="JNZ05_004002"
                     /pseudo
     CDS             261..690
                     /locus_tag="JNZ05_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            877..1041
                     /locus_tag="JNZ05_004003"
                     /pseudo
     CDS             877..1041
                     /locus_tag="JNZ05_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1535..2113
                     /locus_tag="JNZ05_004004"
     CDS             1535..2113
                     /locus_tag="JNZ05_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8566997.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(2222..2480)
                     /locus_tag="JNZ05_004005"
                     /pseudo
     CDS             complement(2222..2480)
                     /locus_tag="JNZ05_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2464..3018
                     /locus_tag="JNZ05_004006"
                     /pseudo
     CDS             2464..3018
                     /locus_tag="JNZ05_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          908 a          565 c          799 g          746 t
ORIGIN      
        1 tgcgcagacc tgaatattcc gttctttgtg gatgccagaa acaaaaaact ggtcttctgg
       61 tttgtacgtg ttgatgacga agggtatccg gaaattgccc gctgcatgga gcgggaattt
      121 gcgacaattc ctgccggtat caacgccgac ggcatgtatt gccctgaatg cggtacggtt
      181 cactggccgg atggcgttat tccgcctttc tgatgcttcc ccgttttgcc gatatttttc
      241 agcagggaaa ccgatggctt aactggctgg agaagcagcc ggtgcagatg agccgcctgg
      301 aacattatgc cggtcaggat gaaattggcc tgcgttacaa cagccaccgg acaaaacgag
      361 aagaaaacct tgtgatgagt ggcgacgagt ttatggaaag gttctcctgg catgtggcgg
      421 ataaggggtt ccggatggtg atacggggtc ctgagtccgg tgaagcggcg attactggaa
      481 gatgtggtgt acgccataac ggagacagtg agaaaaacgg cgaggcaaat cacaaggaga
      541 gggatgtatc agcagtgact gaaggttgac ccgctgaagt gcgagctgcg cggaggtcag
      601 atgcggttta cggggctgaa gaggggttac cgtctggcag agcaggttat gatgcatgag
      661 ccgctggctc gaatacggtg gtgcggctga gagtcgcata ggggaagttg cgtccatgtt
      721 gaggggaacg gagcaaaaaa ccatcagaga tacattgaat caatcaatgt aagcaatatt
      781 aagtgtctat accgattact tgatgatcag gttgtgttga ataaacgcgc attcttcggt
      841 gcttttttaa tcttttgggg atttaaattc ctatcgatga actgtcgata tgaaaaagcg
      901 agcatcatac tgacgtcaaa caaaggggtc gcagactggg gagaaatgtt cggagatcac
      961 gtgctggcaa cagcgatcct agatcggctg ctacacgctg aatatcaaag gagagagtta
     1021 ccggttaaaa gagaaacgta aagctggagt gctgaccaaa aacacaacgc caatcagtga
     1081 tgatgaaatg gtggaaaacg gacagcatca gtaacgaaag tatcttagca ggcatgaaaa
     1141 tcgtccgcat cccctaaacg gtcaaaatgc ttctgccgtg aaggagggag aatgccatct
     1201 catcacttat atctggcgaa tgaaacacag atggtcggcc ttgcccccaa acacacggcg
     1261 gtgcaaaatg gaatggcaga aaacttcgtc aaaacgatga aatatgatta aatcagtgtt
     1321 atgttcgaac ctgacgtgtt aacagcagta aagtagtctc gcgaaggtgt tcaaacatta
     1381 taatgaatgg catcctcata gtgcgcaggg ttatcgcttg ctacggaaat atctgaagcg
     1441 acgtaccagt aatggggaaa atgatagctg gtttctgaaa atatagagga aaatgtgata
     1501 tatatatcct gttaatattt aaggagaata taaaatgaaa gtctcgttta agtctttagg
     1561 ctatattttt catgatatat ataataaaaa acacactatc gatgagttca acgatgtggt
     1621 aagaaaagca gttttatcag gtaaaattaa cgagctgaat gcatgtcata aagtggcaat
     1681 ttttcttgcc gaaaaagaca atgagataac aaagaaagac aaagccaaga taatagatac
     1741 attaactgaa aactattcca ttgaatttca acaattaatg aatattagtg agcgcacgtt
     1801 aaattcatct ttatatatta ctcctggtga aagtggtttt gtcagttttg ttaatcgtga
     1861 aggaaaaata tgtcatactg catatgtaaa atcatcagat aatagcatgg catattacca
     1921 tgcaaatggt agcagcattg acaaatatat tacggatatg tgcggcctta tttgtatgag
     1981 gcatattgag tctactggca ttatttttta tatgcttgat gaaaaagttt tatctgccat
     2041 tgctgaattt atgaatgaaa aaggatggcg ggctgcgttt tgttcagcaa agaacttata
     2101 taaatgtgtt taattttaga aaaactattt ggtaaaatcc aacaagagtc gagaggttcg
     2161 cgttgcttca gcagggcctc agcctgttca accagactca gttgcaggtc gtgacgcaga
     2221 tttagtggtg gtggttgatt gtcagatacc agccgatgat cttgtagtgc atatcatcag
     2281 actttgagaa gcatatggtt ttgcgggtaa gccgcttaag atgtgtccgc aggttcagat
     2341 tatgtcgctc tatccgctga gtgcatttct tgcttataac atgactggtt gaggacaata
     2401 aagttcggta taccggctca ggcatcggtc atgtagaagg caatgctaaa ctggctcagc
     2461 aaactaagag caacgcgcat ccacttaccc aatgcggttg agaaaatagc cttcgatcgc
     2521 tttcatgtgg cgaaacaacc gggtgaggtg gtggataaaa cccgccagaa tgaacatccg
     2581 catcttcctg ttgaaagtcg tcgtcaggca aagggaactc gcttcctgtg gcagcacagc
     2641 gataaatgga tgaccgaatc caggcaggaa aagctgatat ggctgcgtgc gcagatgaag
     2701 ctgacgagcc tgtgctgggc gctgaaagaa ctggcaaagg atatctggag cagaccatgg
     2761 agcgaggaaa gacgcaacga ctggcagaga tggctgcggc caacagtgac gtccccatga
     2821 tgaagaatgt ggctaaaacg atagggaaaa ggttgtacgg tatcctgaac gccatgcgcc
     2881 atggcgtatc aaacggaaat gcggaagcac tgaacagcaa gatcaggctg ctgagaataa
     2941 aagccaaggg ctaccgcaac cgggagcgct ttaagctggg agtgatgttc cactatggga
     3001 aactgaatat ggcgttct
//
LOCUS       DADMEQ010000226         2942 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.228, whole genome shotgun sequence.
ACCESSION   DADMEQ010000226 DADMEQ010000000
VERSION     DADMEQ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.228"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(49..124)
                     /locus_tag="JNZ05_004007"
     tRNA            complement(49..124)
                     /locus_tag="JNZ05_004007"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(89..91),aa:Val,seq:tac)
     gene            complement(169..244)
                     /locus_tag="JNZ05_004008"
     tRNA            complement(169..244)
                     /locus_tag="JNZ05_004008"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(209..211),aa:Val,seq:tac)
     gene            503..1918
                     /gene="gltX"
                     /locus_tag="JNZ05_004009"
     CDS             503..1918
                     /gene="gltX"
                     /locus_tag="JNZ05_004009"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAY8566998.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(1970..2341)
                     /locus_tag="JNZ05_004010"
     CDS             complement(1970..2341)
                     /locus_tag="JNZ05_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY8566999.1"
                     /translation="MTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGGRARLILV
                     DTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTDIEQEKVA
                     QFLSREGIRNFLARLDIDESA"
     gene            complement(2364..2708)
                     /locus_tag="JNZ05_004011"
     CDS             complement(2364..2708)
                     /locus_tag="JNZ05_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY8567000.1"
                     /translation="MTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKARLVHV
                     NEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLLREGII
                     GLLQRLGIRDSK"
BASE COUNT          726 a          719 c          736 g          761 t
ORIGIN      
        1 aattgattaa aagtcaactg ctctaccaac tgagctaacg acccgaagtg gtgggtgatg
       61 acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc tgagctaatc
      121 acccacttct caatttcttg ctacacggcg gagactacat aaagtagttg gtgggtgatg
      181 acgggatcga accgccgacc gcctccttgt aagggaggtg ctctcccagc tgagctaatc
      241 acccccgctg tgtggagtcg cattataggg agagttcaaa atgagtcaac gcattttcta
      301 aagaaattgt tcgttcgtcg taaatttaag caagatgatc gcaaaacaga ccgtgttgcg
      361 caatttgtca acgaaaacaa taatgcgtaa ggtagaaacc cgaactacat tgaggaatca
      421 ggcgggagtg atagaatatc gcccactcaa tttttccagg atttgccggt tgtcggcatc
      481 tttctaaacg taaggccatt tcatgaaaat caaaactcgc ttcgcgccaa gcccaacagg
      541 ctatctgcac gttggcggcg cgcgtactgc tctttactcc tggctttttg cacgtaacca
      601 cggcggtgag ttcgtgctgc gtattgaaga caccgatctt gagcgttcca cgccggaagc
      661 tatcgaagcc attatggatg gcatgaactg gctgagcctg gagtgggatg aaggtccgta
      721 ctaccagacc aaacgttttg atcgctacaa cgcggtgatc gatcagatgc tggaagaggg
      781 cactgcttat aaatgctatt gctctaaaga gcgcctggaa gcgctgcgcg aagagcaaat
      841 ggcgaaaggt gagaagccgc gttatgacgg tcgctgccgc cacagccatg agcatcatgc
      901 tgatgatgaa ccgtgtgttg tacgttttgc taacccgcag gaaggttctg ttgtttttga
      961 cgatcagatc cgtggtccga tcgagttcag caaccaggaa ctggacgatc ttattatccg
     1021 ccgtaccgat ggttccccaa cctataactt ctgtgtggtt gtcgatgact gggatatgga
     1081 aatcacccac gttatccgtg gcgaagacca tatcaacaac acgccgcgcc agatcaacat
     1141 tcttaaggcc ctgaaagcgc cggtgccggt ttacgcgcac gtttctatga tcaatggcga
     1201 tgacggtaaa aaactgtcca aacgtcacgg ggcagtcagc gtaatgcagt atcgtgatga
     1261 cggttatttg ccagaagcac tactgaacta tctggtgcgt ctgggctggt cccacggcga
     1321 tcaggaaatc ttcactcgtg aagagatgat caaatacttc actttgaatg ccgtcagcaa
     1381 atctgccagt gcgttcaaca ccgacaagct gctgtggctg aaccatcact acattaacgc
     1441 gctgccgccg gagtatgttg ctactcactt acagtggcac attgagcagg aaaatatcga
     1501 tacccgtaac ggcccgcagc tggctgatct ggtgaaactg ctgggcgaac gctgcaagac
     1561 gctgaaagag atggcacaga gctgccgtta tttctacgaa gattttgctg agttcgatgc
     1621 cgatgcggcg aaaaaacatc tgcgtccggt agcgcgtcag ccgctggaag tggttcgtga
     1681 caaactggcc gcgattactg actggaccgc tgaaaacgtt catcacgcta ttcaggcgac
     1741 ggcggatgag ctggaagtgg gtatgggtaa agttggtatg ccgctgcgtg tcgccgtaac
     1801 cggtgcgggg cagtctccag cactggatgt taccgttcac gcaattggta agacccgcag
     1861 tatcgagcgt atcaacaaag cgctggattt tattgctgaa cgcgaaaatc agcagtaatt
     1921 agcgtgtaaa aagataaacg gcaggagata atatcctgcc gttttttatt tatgctgatt
     1981 cgtcaatatc gagacgggcg aggaaattgc gaattccttc acgtgacaaa aattgcgcga
     2041 ctttttcctg ctcaatgtct gtcatattat ctatcgcgtt gatgatttgt cgatacgccg
     2101 gggcgcgtgc tccaggagcg tattcagcaa ttctctcttc agcttccatc aacattggtg
     2161 tgttatggaa ggctggcgtg ttctgaataa actcgcaaac ttgcgtatcg accagaataa
     2221 gtctggcacg accgcctttc acgccgggaa atttttccgt tttccagcct ttttctctga
     2281 tccaacggtt aacagtttgt ttagccacac cgaggcattc agccagttct tcggtggtca
     2341 ttttattgcg taatcttttc atattatttg ctatcgcgaa tccctaagcg ttgtaacaat
     2401 ccgataatcc cttcccgcag aagcaaggat gtaaactgtt tttgttcaac tggcgtcatc
     2461 tctttcgcca gtgtcaccag caatacttca agcggcgcat caccagaaag ggcaggcgct
     2521 tctccctgac cttctggacg ttcagcattg cgaatatatt cacgaacctg ttcattgacg
     2581 tgaaccagtc gggctttgcc accctggacg ccaggttttg gtgacgttgt ccagccttcc
     2641 ttgcgtaccc atttattaat ggtctggcgg ctatagccgg tcagtctggc aagttcatct
     2701 ggtgtcatcc tctccttgga cataacaatt ccctgaatgt atgtgggtct attagtagtt
     2761 cattttataa caccacttta caggaaaccg tgacatgaat aactactgaa tgcgagcggt
     2821 tttccaaaat tgttcatttg cctgattttt cagcgattga aactacacgt tgcaatttgc
     2881 cgttgacaca ttcgggcgga attcatatga tgccgcccgt caactcgaca agctttacgt
     2941 ga
//
LOCUS       DADMEQ010000227         2797 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.229, whole genome shotgun sequence.
ACCESSION   DADMEQ010000227 DADMEQ010000000
VERSION     DADMEQ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.229"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            64..447
                     /locus_tag="JNZ05_004012"
     CDS             64..447
                     /locus_tag="JNZ05_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567001.1"
                     /translation="MPTRKSTKNSIKNYLNNFDSYEQLPSKLDDIFIGFCRGEGHPKY
                     NKKLFYLLKNLDEINASSVTNRLQRQATRLSYELPTDAYCALLAVMCAKLIGIVEHHI
                     AVGNIEPMENEQPDFEFDPYILEEF"
     gene            449..730
                     /locus_tag="JNZ05_004013"
     CDS             449..730
                     /locus_tag="JNZ05_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567002.1"
                     /translation="MGNKHTLTRVTKTAFEELNRIKNITGLPHSAVMKLAINRDINEQ
                     DLLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLAMEV"
     gene            730..1116
                     /locus_tag="JNZ05_004014"
     CDS             730..1116
                     /locus_tag="JNZ05_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567003.1"
                     /translation="MYDFYKHRDHLVRFDGKVWKDVTQTNKPKFTHQQLASRFDIWAE
                     AQKDNGDFEFIRGFDYAYESDLKDAIELMENFNSIKNSDKHKANGNRYDKDLIIDAIT
                     KGQSQSYISEKYGLSRSQYYKIKASV"
     gene            1255..1578
                     /locus_tag="JNZ05_004015"
     CDS             1255..1578
                     /locus_tag="JNZ05_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567004.1"
                     /translation="MNNFPLQIFIDTDTAMMIQAFTDVGVSIDFDKLLELMADNAESI
                     SDFIHSVEFNEPRMMLPITDSNMKRLVIEQTNKYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            2052..2792
                     /locus_tag="JNZ05_004016"
     CDS             2052..2792
                     /locus_tag="JNZ05_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567005.1"
                     /translation="MSIRDEHEAIVDRKPCAYDLHFTDEGDEYAHMKPYHKIIAKLAD
                     EEAKKERLRKIVPANNLEYINPEPLEMTDVCKTEALIALERKYYPLLKEQRIKLDNAY
                     DVVKNFEETQKPSEYDIEYELKTNPFIYYEGNYNNGFGTARENVPKVLQSIPDGFRLM
                     DVRQASRGTGSYILMTNKTDEEILQLAEANLTKHYSDKLDKLKQKLKKELDTMKELVS
                     EYESQKKIAMQADIEQLVKISQKYAKAL"
BASE COUNT          948 a          482 c          561 g          806 t
ORIGIN      
        1 acccaaaaag aacgcaaagg cattgacgaa gtaattcggg caattacacc gagggaacgt
       61 tatatgccaa ccagaaagag cacgaagaac agcatcaaaa attatttgaa taacttcgac
      121 agctacgagc aactaccaag taagcttgat gatattttca ttggattctg tagaggtgaa
      181 ggccacccga aatacaacaa aaaactattt tatcttctaa agaatcttga cgaaattaac
      241 gcatccagcg tgacaaatcg tcttcagcgt caagcaacca gacttagcta cgaattacca
      301 accgatgcct attgtgcatt gcttgctgta atgtgtgcaa agctaattgg tattgttgaa
      361 catcatattg ctgttggtaa tattgagcca atggaaaacg agcaaccaga ctttgagttt
      421 gacccgtata ttttggagga attttaaaat gggaaataaa cacacattaa ccagagttac
      481 aaagacagcc tttgaagaac ttaaccgaat caaaaacatt acaggtctgc cacactcagc
      541 agtgatgaag cttgcaatta accgtgatat taacgaacaa gatttgctta atagtcgctt
      601 agaggcagca agacaccata tcaggattaa gcaagatgac cttgatagtc tgaaatcttt
      661 aaatgctttt ggactggatt tagcaaggtt actaagtatc aaaattcaca aattagcgat
      721 ggaggtttaa tgtatgactt ttataaacat cgtgaccatt tggtaagatt tgacggtaag
      781 gtctggaaag acgtaacaca aaccaataag cccaagttta cccaccagca attagcctca
      841 agatttgata tctgggctga agcacaaaaa gataatggtg atttcgagtt tatcagaggt
      901 tttgattatg cttacgaaag cgaccttaaa gacgccattg aattaatgga gaacttcaac
      961 tctattaaaa atagtgacaa gcacaaagcc aacggtaaca gatacgataa agatttaatc
     1021 attgatgcaa tcaccaaagg acaatcacaa agttatattt ctgagaaata cggcttatca
     1081 cgtagtcaat actacaagat taaagcgtct gtgtaagtct gtgagaatga gtgagactca
     1141 aagatgagct accctattac taaggtgtct cactccctct gtaacccgca tggttactgg
     1201 cctcagaatg gctattttgg cagatttaag atgtgttttt ataggatttt aaaaatgaat
     1261 aatttcccac tacagatttt tattgatacc gacaccgcca tgatgattca ggctttcact
     1321 gatgtaggtg ttagcattga ctttgataag ttgttagagt taatggctga taatgcggaa
     1381 agcatctctg actttattca ctcggtggag tttaacgagc ctcgaatgat gttaccaatt
     1441 acagacagta atatgaaaag attggttatt gagcaaacga acaaatacag tatttcccca
     1501 gagcaatttt tgaaaggtgc tgtgacaatc ctgtacagcg ataatatttt ggttacggat
     1561 tccgtgaggg ttcactaatg ggcttaataa ttacttggac gttgcttggc ttatttgttg
     1621 gttgctgggt gggtaacaca gtaaataaga attgatgtta tcaatatgtt aaaagctgtt
     1681 gacaaataac cttaagcatg agatgatggt aataggggag agcaactctt cctgaattaa
     1741 actattaatc attctacatc aatgatagtt attctgagtt ggaaaggtgc aatgcttttc
     1801 gtcatggcct atttagccca cactagatag gctatctcaa aaagtctttg ttcattcgtt
     1861 attgaatcct ttggatgttc tttaatttct tcaccttagc cctttatagc gttttagtta
     1921 tctagggctt tttcagcttt taaaaagcgc acttggcaaa ccgcctttta agtctcttct
     1981 aagttcgtag gctgtcaggt gtgcttttta aaaactgaaa cataaattat cttagaagag
     2041 gaaaaataat aatgtctatc cgtgatgaac atgaagcgat tgttgataga aaaccttgtg
     2101 catatgattt gcattttact gatgaaggag atgagtatgc acatatgaaa ccttatcaca
     2161 aaattattgc caagcttgca gatgaagagg ccaaaaaaga aaggctgcga aagattgttc
     2221 cagccaataa tcttgaatat atcaatcctg agcctctgga aatgactgat gtttgcaaga
     2281 cagaagctct gattgcactt gagaggaagt actatccact actaaaagag cagcgcatca
     2341 agcttgataa tgcttatgat gttgtcaaaa acttcgaaga aactcagaaa ccttcagaat
     2401 atgatattga atatgagctt aagacaaacc cgtttatcta ttacgaagga aattacaaca
     2461 atggtttcgg tacagcaaga gagaatgttc caaaagtgct tcaaagtatt cctgacggtt
     2521 ttcgattaat ggatgtcaga caagcttcac gtggtacagg ctcatatatc ctgatgacta
     2581 acaaaacaga tgaagagatt cttcagttag ctgaagctaa tctgactaag cattactccg
     2641 ataagctgga taagttaaag caaaagctta aaaaagagct tgatactatg aaagagcttg
     2701 ttagtgaata tgaatcacag aagaaaattg ctatgcaggc tgatattgag cagttagtga
     2761 agatttctca gaaatatgca aaggcacttt aactatg
//
LOCUS       DADMEQ010000228         2795 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.230, whole genome shotgun sequence.
ACCESSION   DADMEQ010000228 DADMEQ010000000
VERSION     DADMEQ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.230"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..388
                     /locus_tag="JNZ05_004017"
     CDS             <1..388
                     /locus_tag="JNZ05_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="required for the translocation of capsular
                     polysaccharide through the outer membrane; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HAY8567006.1"
                     /translation="NDDLKVFVMGEVGKQSTLKMDRSGMTLAEALGNAEGMNQDVADA
                     TGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQLEPYDIVYVTTAPLARWNRV
                     ISLLVPTISGVHDLTETSRWIQTWPN"
     gene            376..822
                     /gene="etp"
                     /locus_tag="JNZ05_004018"
                     /gene_synonym="yccY"
     CDS             376..822
                     /gene="etp"
                     /locus_tag="JNZ05_004018"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HAY8567007.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            842..>2795
                     /gene="etk"
                     /locus_tag="JNZ05_004019"
     CDS             842..>2795
                     /gene="etk"
                     /locus_tag="JNZ05_004019"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HAY8567008.1"
                     /translation="MTTKNMNTPLGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMLPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPM"
BASE COUNT          737 a          661 c          747 g          650 t
ORIGIN      
        1 caacgatgat ctgaaagtct tcgtgatggg tgaagtgggt aaacagagca cgctgaaaat
       61 ggaccgcagc ggtatgaccc tggcagaagc gctgggtaac gctgagggta tgaatcagga
      121 tgtcgctgat gcgacgggga tttttgttat tcgtgcaacg caaaacaagc agaatggcaa
      181 aatcgccaat atttatcagc tgaatgcgaa ggacgcatcg gcaatgatac tcggaacgga
      241 attccagctt gaaccttacg acattgttta tgtcactact gcgccgctgg ctcgttggaa
      301 ccgtgtgatt tctctgctgg taccgacgat ttccggggtg catgacctga cagagacttc
      361 acggtggatt caaacatggc ccaactaaaa tttaactcaa tcctagtggt ttgtaccggc
      421 aacatttgcc gttcgcccat tggcgagcgc ttgctgcgta aacgtctgcc gggcgtaaaa
      481 gtcaaatcgg caggtgttca tggtctggta aaacaccctg cagatgcgac agcggcagat
      541 gttgccgcta atcatggcgt atctcttgaa ggacatgccg gacgtaagct cactgctgag
      601 atggcacgaa attacgatct gattctggca atggagtcgg aacatattgc tcaggttacc
      661 gcaattgctc ccgaggtccg cgggaagaca atgctgtttg ggcaatggct ggaacagaaa
      721 gagatcccgg atccctatcg taaaagtcag gacgcatttg aacatgtcta cggtatgttg
      781 gagcgcgcca gtcaggaatg ggcgaagcgc ctcagccggt aatttgagtt tataaaatac
      841 gatgacaact aaaaatatga atacgccact aggcagcact caggaaaatg agatcgatct
      901 gcttcgtctg gtcggcgagt tatgggatca ccgtaagttt attatcagcg tgaccgcgtt
      961 attcacgctg atcgctgtcg cttactcgct gttaagcaca ccaatttatc aggcagatac
     1021 tctggtccag gttgagcaaa aacagggcaa cgccattctc agcggcctga gcgatatgct
     1081 ccctaactca tcgcccgagt ctgcaccgga gatccaactg ctgcaatcgc gcatgattct
     1141 cggtaaaacc attgctgaac tgaatctgcg cgacatagtt gagcagaagt attttccgat
     1201 tgtgggtcgc ggctgggcga gattaaccaa agaaaaacca ggtgagctgg cgatcagctg
     1261 gatgcatatt ccacaactga atggtcagga tcagcaactg acactcacgg ttggggaaaa
     1321 cggccactat acactggaag gtgaagagtt caccgtcaat ggtatggtcg gccagcgtct
     1381 ggaaaaagat ggcgttgcgc tgactatcgc ggacattaag gccaaaccag gaacacagtt
     1441 tgtcctgagc cagcgtaccg aactggaagc gattaacgca ttgcaggaaa cctttaccgt
     1501 tagcgaacgc agtaaagaaa gcgggatgct ggaacttacc atgactggtg atgatcccca
     1561 gttgattact cgtattctga acagcatcgc taacaactat ttgcaacaga atatcgctcg
     1621 ccaggcggcg caggattcac aaagccttga attcttacag cgccagttac ctgaagtgcg
     1681 cagcgagctg gaccaggcgg aagaaaaact caacgtttat cgccagcagc gcgattcggt
     1741 tgaccttaac ctggaagcca aagccgttct tgagcagatt gtgaacgttg ataatcaact
     1801 caatgagctg accttccgcg aggcagagat ctcccagctg tataagaaag atcacccaac
     1861 ttatcgtgcg ctgctggaaa aacgccagac gctggagcaa gaacgcaaac gcctgaataa
     1921 gcgggtatcg gcaatgcctt ccacccaaca ggaagtgttg cgtttaagtc gtgacgtaga
     1981 agcgggccgt gcggtatatc tgcaattact taaccgccag caggagttga gtatttcgaa
     2041 atccagtgcc attggtaacg tgcggattat cgacccggca gtcactcagc cgcagccagt
     2101 gaaaccgaaa aaagcgttga atgtggtgct tggttttatt cttggcctgt ttatttcggt
     2161 gagtgccgtg ctggcgcgtg cgatgttgcg tcgtggtgta gaagccccgg aacaactgga
     2221 agagcacggc atcagcgttt attccactat cccaatgtcc gagtggctgg ataaacgcac
     2281 ccgtctgcgt aagaaaaatt tattttctaa tcagcagcgc catcgtacta aaaatatccc
     2341 cttcctggcg gtggataacc cggcggattc tgctgtggaa gccgtacgtg cgctacgaac
     2401 cagtctgcat ttcgctatga tggagactga gaataacatt ctgatgatca ccggtgcgac
     2461 gccagacagt ggtaaaacgt ttgtcagttc aactctggca gcggtgatcg cccagtccga
     2521 tcaaaaagtg ttatttattg atgccgactt acgccgtggt tattcgcata acctgtttac
     2581 cgtgagtaat gaacatggct tgtcggaata tctggcaggt aaagatgagc tcaacaaagt
     2641 gatccagcat tttggcaaag gaggctttga tgtgattact cgcggtcagg tgccacctaa
     2701 cccgtctgaa ctgctgatgc gcgatcggat gcgtcaatta ctggaatggg cgaacgacca
     2761 ttacgatctg gtgattgtcg atacgccgcc gatgc
//
LOCUS       DADMEQ010000229         2789 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.231, whole genome shotgun sequence.
ACCESSION   DADMEQ010000229 DADMEQ010000000
VERSION     DADMEQ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.231"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            23..985
                     /gene="yeeE"
                     /locus_tag="JNZ05_004020"
     CDS             23..985
                     /gene="yeeE"
                     /locus_tag="JNZ05_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HAY8567009.1"
                     /translation="MYIAKNNRMFYALLIAISAQSVGVFALIQAGLLTYEAGAFPWLG
                     TVIGGYLFGLGIVLAGGCATGTWYRAGEGLIGSWIALFTYMVMSAVMRSPHASGLNQT
                     LQHYTTEHNSIAETFNLSVWPLVTVLLVITLWVVMKELKKPKLKVATLPPRRTGIAHI
                     LFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGLGVTSPTANILQFLVAGDVKYINW
                     GVFLVLGIFVGAFIAAKASREFRVRAADAQTTLRSGLGGVLMGFGASIAGGCSIGNGL
                     VMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRKARLATAAAN"
     gene            999..1226
                     /locus_tag="JNZ05_004021"
     CDS             999..1226
                     /locus_tag="JNZ05_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HAY8567010.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1269..2693)
                     /gene="sbcB"
                     /locus_tag="JNZ05_004022"
     CDS             complement(1269..2693)
                     /gene="sbcB"
                     /locus_tag="JNZ05_004022"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HAY8567011.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWQEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
BASE COUNT          643 a          660 c          742 g          744 t
ORIGIN      
        1 cctgactggt ggttttcgcg atatgtatat cgcgaaaaat aatcgcatgt tttacgcctt
       61 gctgattgca atttcggcac aaagcgtggg ggtttttgcg ttaattcagg cgggcctact
      121 gacttacgaa gccggggcgt tcccgtggct tggtactgtt ataggtgggt atcttttcgg
      181 gctgggaatt gttctggcgg gtggatgtgc caccgggacc tggtatcgcg cgggtgaggg
      241 attgatcggc agttggatcg cgcttttcac ttatatggtg atgagtgcgg tgatgcgttc
      301 tccacacgcc agtggtttaa atcaaacctt gcagcactac actactgaac ataactctat
      361 tgctgagact tttaatttgt ctgtgtggcc gttggttacc gttttgctgg tgataacgct
      421 ctgggtggtg atgaaagagt tgaagaagcc aaaactcaaa gtcgcgacct taccgccgcg
      481 ccgaaccggg atcgctcata ttctgtttga aaaacgctgg catccctttg tcactgctgt
      541 actcatcggt ttgattgcgc ttttagcctg gcccctgagt gaagcaaccg gacgcatgtt
      601 tgggttagga gtcacttccc caacggccaa tattctgcaa tttctggtcg cgggtgacgt
      661 gaaatacatt aactggggcg ttttcctggt gttagggatc ttcgtggggg catttattgc
      721 agccaaagcg agccgtgagt tccgcgttcg cgcagctgat gcacaaacaa cattacgtag
      781 cgggttaggt ggtgtactga tgggcttcgg tgccagtatt gcaggtggtt gctctatcgg
      841 taatggactg gttatgactg caatgatgac ctggcagggc tggattggcc ttgtatttat
      901 gattctcgga gtctggactg cgtcctggct tgtgtatgtt cgaccgcagc gtaaggcgcg
      961 actggcaact gctgcggcaa attaagtaag gtgtaaagat ggcgattaaa aagctggatg
     1021 tggtgacgca ggtctgtccg tttccactca tagaagcaaa agcagcactg gcagaaatgg
     1081 ccagcggcga tgagttggta attgagtttg attgtaccca ggcaactgaa gcaatcccac
     1141 agtgggcggc agaagaagga catgccatca ccgattatca gcagattggc gatgcagcgt
     1201 ggagcatcac cgttcaaaaa gcctgactca acattgtcct ccgccgtacc agcggcggcg
     1261 gcttcaaatt agacgatctc ttccgcgtac tgccaaagtg cttttaacag cgccactttc
     1321 tctttgtcat cggcatattg ttgtaccagc atctgcaatt catcagcata accctgcaaa
     1381 aactctggcg tgaagacctg gtggcggtgc tcctgccagc gttgctgctc ggcataatcc
     1441 agcgtccccg ggaagttgcg tgcccgataa ttgaacaaca acttttcaat tcgtttatca
     1501 acaaaagtga tatccagtgc cggtaaatta cgcggctcgg tttccagcac aattttcatt
     1561 gctgcacgat cggcgtcact gaaaaagcca ttataaagct gtgcatccac attatctgaa
     1621 ggtgtaaacg gttcggcttc cgcgaatatc gctaccactt tttcacgcac ttgcggattt
     1681 tcacgcagaa tttttaggtt atcgaggcaa tgctgacgat taattcccag tcggtcggca
     1741 tcttccgggc gtagcgtatt cgcctgggcc agtaccggac atttattgat atgcaccagc
     1801 ttaaccggaa cggcggcgtt atcgccaaga tcggcttttg cggtatataa acgctcgcgc
     1861 aatgtgtcga tatccagctc cagtaatggc gaaatgtctc ctgccaaatc cgccataatt
     1921 acggcattgc gattttcagg atgccacgcc agcggtgcca cccagctggt attgccacgc
     1981 catgccccaa acattccgga aacgtgagcc aggggtttca tctgcggaac atcaatcaac
     2041 gccatcagtt tgtgtttatt acgatgggta aagagataat caaacaggcg tggctgacgc
     2101 gtttttacca gtttcgccat cgcaatagtg gcgtacacat cagccatcgc atcgtgggcg
     2161 ttgctatgtt caataccatt cgctttggtt aaatgctcaa ggcgaaagct cggtagaccg
     2221 tcatcatttt caggccagtt tattccttcc gggcgcaggg cataacaggc acgcataaca
     2281 tccagtaaat cccagcgcga gttatcatgc tgccagctcc aggcgtaagg atcgtagaaa
     2341 ttacgataaa aaacgttgcg tgtgacttcg tcgtcgaaac gcacattgtt gtagcccaga
     2401 atacaggtct tcggtacggt aaaaagcgag tgaatacggg cggcaaacgc ggcttcgttt
     2461 tctcctttcg cccgtgcctc ctgcggggta ataccggtaa ttaatactgc tccaggccgg
     2521 ggtaaatagt catccgcggg cttgcagtaa aagacttcgg gttcgccgat gacattgaat
     2581 tcgtcatcgg tgcgaatggc tgcgaactgt gcagggcgat ctaacgcggg gtgcgtgcca
     2641 aaggtttcgt aatcgtgaaa gaggaaggta ggttgtttat ctgtgtctgt cattgtctag
     2701 cattgtccac tgtagttaat taactgtctg ttataactcg aaaagcaacc cttttttcac
     2761 ctgagtatgt ctacgaacgt caaatatag
//
LOCUS       DADMEQ010000230         2622 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.232, whole genome shotgun sequence.
ACCESSION   DADMEQ010000230 DADMEQ010000000
VERSION     DADMEQ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.232"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..104)
                     /locus_tag="JNZ05_004023"
     CDS             complement(<1..104)
                     /locus_tag="JNZ05_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HAY8567012.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(145..600)
                     /gene="csgB"
                     /locus_tag="JNZ05_004024"
     CDS             complement(145..600)
                     /gene="csgB"
                     /locus_tag="JNZ05_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HAY8567013.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1354..2004
                     /gene="csgD"
                     /locus_tag="JNZ05_004025"
     CDS             1354..2004
                     /gene="csgD"
                     /locus_tag="JNZ05_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HAY8567014.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2009..2398
                     /gene="csgE"
                     /locus_tag="JNZ05_004026"
     CDS             2009..2398
                     /gene="csgE"
                     /locus_tag="JNZ05_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HAY8567015.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            2423..>2622
                     /locus_tag="JNZ05_004027"
     CDS             2423..>2622
                     /locus_tag="JNZ05_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="nucleator protein that participates in the
                     polymerization of curlin (CsgA) subunits into curli
                     (extracellular fibers from Escherichia and Salmonella spp.
                     that are involved in the colonization of inert surfaces
                     and biofilm formation); part of the curli secretion and
                     assembly protein complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgF"
                     /protein_id="HAY8567016.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSA"
BASE COUNT          768 a          552 c          497 g          805 t
ORIGIN      
        1 ccaccaccgt ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg
       61 gagaatacga ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg
      121 attgatttaa aagtcgaatg gtaattaacg ttgtgtcacg cgaattgcca tttgcgactg
      181 tctctgcact acaattgccg ttttttgagt accatactgt gtaatatttg ctttattacc
      241 agaacctttc tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc
      301 gttggcactg cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt
      361 tgcccgattg ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg
      421 taactgagca ctattattag tcccagcttg accaattatg gctgcctgat taaatgaaga
      481 cttactcaat tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc
      541 ggctgcaatc ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat
      601 gttgtcaccc tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta
      661 acgcaacctg tattttgtta acgctgcgtt acgatggaaa gtatgtctgc ggaaatattt
      721 ttaataactc acccgcgcgt tgtatttttc tttttttgtt cataccaaag tattaaaacc
      781 cgcgtaaata cgctgttatc tacgcaaaaa atattttgtt ttctcttaaa tctccgtttt
      841 ccgctatcaa aaatcaccag acagtcattc ttcttgcccg tcgctgattg ctgcgatatg
      901 tcttgcgcac aagccgtgac aaacctcatc tttttgcagt aaaaaaattg tccacggagg
      961 tgtggagaaa aaacaaaaac gttttacatg acgaaaggac tacaccgaaa tattttttat
     1021 atgtattatt agtaagttag caccatttgt atgatttttt aaaattgtgc aataaaaacc
     1081 aaatgtacaa cttttctatc atttctaaac ttaataaaac cttaatgtta acattttaat
     1141 ataatgagtt acattttgtt acatgtttaa cacttgattt aagatttgta atgcctagat
     1201 tgaaatcaga tgtaatccat tagttttata ttttacccat ttagggctga tttattacta
     1261 cacacagcag tgcaacatct gtcagtactt ctggtgcttc tattttagag gcagctgtca
     1321 ggtgtgcgat caataaaaaa gcggggtttc atcatgttta atgaagtcca tagtattcat
     1381 ggtcatacat tattgttgat cactaaacct tctttgcagg cgacagctct cttgcagcac
     1441 cttaaacaat cgctagcaat tacaggaaaa ttacataata ttcaacgttc tctggacgat
     1501 atctcttcag gctctattat tcttctggat atgatggaag cggataaaaa gcttatccat
     1561 tattggcagg atactttgag caggaaaaac aacaatatca aaatattgtt gctaaatacg
     1621 cctgaagatt acccgtaccg cgacattgaa aactggcctc atatcaacgg cgttttttat
     1681 gccatggagg atcaagaacg tgttgtcaat gggttgcaag gcgtcctgcg tggcgaatgc
     1741 tactttacgc aaaagcttgc cagttacctg attacacatt ctggtaacta tcgttataac
     1801 agcacggaat cagccctcct tactcatcgg gaaaaagaga tcctgaataa gctgcgtatc
     1861 ggcgcgtcta ataacgagat cgctcgttcg ttgttcatca gcgaaaatac ggttaaaacg
     1921 catctttata atcttttcaa gaagatagcc gtaaaaaacc ggacacaagc ggtttcctgg
     1981 gcaaacgata acctcaggcg ataaagccat gaaacgttat ttacgctgga ttgtggcggc
     2041 agaatttctg ttcgccgcag ggaatcttca cgccgttgag gtagaagtcc cgggattgtt
     2101 aactgaccat actgtttcat ctattggcca tgatttttac cgagccttta gtgataaatg
     2161 ggaaagtgac tatacgggta acttaacgat taatgaaagg cccagtgcac gatggggaag
     2221 ctggatcaca ataacggtca atcaggatgt tattttccag acttttttat tcccgttgaa
     2281 aagagacttc gagaaaactg tcgtctttgc actgattcaa actgaagaag cactaaatcg
     2341 tcgccagata aatcaggcgt tattaagtac gggcgatttg gcgcatgatg aattctaaat
     2401 aaaaaattgt tcggaggctg caatgcgtgt caaacatgca gtagttctac tcatgcttat
     2461 ttcgccatta agttgggctg gaaccatgac tttccagttc cgtaatccaa actttggtgg
     2521 taacccaaat aatggcgctt ttttattaaa tagcgctcag gcccaaaact cttataaaga
     2581 tccgagctat aacgatgact ttggtattga aacaccctca gc
//
LOCUS       DADMEQ010000231         2568 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.233, whole genome shotgun sequence.
ACCESSION   DADMEQ010000231 DADMEQ010000000
VERSION     DADMEQ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.233"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(1..2131)
                     /locus_tag="JNZ05_004028"
                     /pseudo
     CDS             complement(1..2131)
                     /locus_tag="JNZ05_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            complement(2275..>2568)
                     /locus_tag="JNZ05_004029"
     CDS             complement(2275..>2568)
                     /locus_tag="JNZ05_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HAY8567017.1"
                     /translation="PYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMG
                     RYVIYHTGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
BASE COUNT          605 a          686 c          666 g          611 t
ORIGIN      
        1 attcgacatt gaccagttca ccaggctggt aatgggtttt gtccgttttc acccggatat
       61 caagctgagg aacggcaact ttgatcccgg cgttctgaaa actgtactga ccattacggg
      121 tatacagcac cgaaaaagtg atgttaggcg caaaggaatt gctcactgga acccgggctt
      181 catacagggt atcgtttaaa cgttgtagcg ttagccagtt tgccggatgc gaaagcagcg
      241 actgctgttc cacgcgatcg cgttccagcg tcaataacgc ttcatcaatt ggctccggga
      301 aagtaatcag catcttcgcg gtttcgcctg gctggtacag cgttttatcc gccacgatat
      361 ctaccgtacc agtatgcgcc gtgctgccct taccgctgac ggcatgactt aacccagcga
      421 gaattaagcc gtctttatcg cgtaatgtca gattgtagtt gccaggttta gcgaaattga
      481 cggtaaagga tttgccgcct gacggtagct ctccgctatg gctcgtgcgg tcttcgagac
      541 gcaaccattc atacgtaaca ggaacctgtt ttgaagattc cagcgcggca taacggaaca
      601 caaccgactc gccgctatta ctgtattgtg cggcggtact taaagagtaa tgcgccaggc
      661 cgcgttcaat gaggatctct ttggtggtgg tgacgcgata cgccgcgccg tcactggcgg
      721 agacggttaa caaatagcgg ctcggtttat cggcggcggg gagatttaac gccacatgac
      781 cgctggcgtc ggacaccgtt tcgctgcctt ccagcgacac ggggaaacgt ccggcataac
      841 gcaaatcgtt accgaccatt gataattgct gagcgcgcaa acttaactgc acgcgggcat
      901 tttttaccgg ctcgccatcc gggtagagca gttgcagttt gccgctgacc gcttcgccag
      961 ttttgaactc ttttttgtcg agagctaaac caatctcgaa atgtggcttg atgcagtttg
     1021 ccacgcgaaa actgctgcta tagacctgat tgcggtaagc aagacgtaac tcataacctc
     1081 cggcaacggc attttctggc aggcggaaac ttccctgccc gccattgcgc gcatccagcg
     1141 tgacatcgac ggtttgcaac agactgccgt tagcgtccag caccgaaagc ttcgccgggg
     1201 cgctgacgat gggggatgaa tgcaacggat cgcggaactc gcggcccatc actttaacat
     1261 cgacacgatc gcctgcgcga tatagcgggc gatcggtaaa aatatacaag cgggtgttgt
     1321 agatttcgct ttcgtagaag aagttctcgg agacaaaaac gccgccttca gcatccttac
     1381 ccagaatgta tgaacgttct ggcgatatat gttgtaactg caaagtaccg ctgtcatcgg
     1441 tcaccccgcg ggtcatcacg ccaagaccgt cagtccacaa gatctctgag ccgggcttcg
     1501 cttcaccctg ttttttaccc gcggtccata ccagaagctc gttacctgac actttgctaa
     1561 gcgccacggt atcggaaaca aacaccaccg tcgtcgcccg atacccacct accatcgcct
     1621 cgacgaggta cagccccggc tcttgttggc cgagaggaat ataaatatta cccggctgcg
     1681 gcgagatgaa attgctggat gcgccttcca gttttatccc ttgctgcggc tcgaccggtt
     1741 tagcctgcca tagtggataa cggaactgtt ccaccaaggg atattttttc agcggggaaa
     1801 actggttgtt ctgtacataa cgggaaggtt taataatggc attgccgaga tgtaattcgg
     1861 gtaatgcctg agtcacattc tgccgtgact gagaagagaa agtacgctgc atcacgcggc
     1921 gagatttgcc gtaccagtta tcccacagct aggtcagcgt attgttcagc ccgtcgccca
     1981 gatattgcgg ttgcaccaca atgcgatgca ggtttttctg ctggcgcaaa aatgccatcg
     2041 ggtcaggaat acgatacagg cgaacgtcca cgccgccgta ctcttccatc tgatagcgcc
     2101 gataatcacg ccccggcgct tccagtcgca ctttcgcctc ttcactgctg ctaaaactgc
     2161 tgtcagcaag caaaaagaat gttcccccgg cgggcggcgc atagttactg gaaggaagcg
     2221 aatcatcagc tttagcaagc cctgttccca caagagacag ggacaagcac gctgctatcg
     2281 cgccagaaaa tttaaacgat aaatgccaat gaaattggga ttggaatcgt tgggtatcca
     2341 tcgggtgtcc ttccatgtca taagttgttg cagactgact gcgcgcattc cgttgtcagt
     2401 tttcgtggcg cttccggtgt ggtagatgac gtaacgcccc atccagacca ttaagtgctg
     2461 ggcatcgccc tgatcgaaaa aaatcatatc gccaggcagc gcctggttta tgtcttggcc
     2521 aataaactgg ctgttgtact gaatcaaatt aatcgcggtc acgtaggg
//
LOCUS       DADMEQ010000232         2503 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.234, whole genome shotgun sequence.
ACCESSION   DADMEQ010000232 DADMEQ010000000
VERSION     DADMEQ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.234"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            155..1108
                     /locus_tag="JNZ05_004030"
                     /pseudo
     CDS             155..1108
                     /locus_tag="JNZ05_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1149..2144)
                     /gene="pgl"
                     /locus_tag="JNZ05_004031"
     CDS             complement(1149..2144)
                     /gene="pgl"
                     /locus_tag="JNZ05_004031"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HAY8567018.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
BASE COUNT          691 a          615 c          561 g          636 t
ORIGIN      
        1 tattgcgata tggttacatt aaggacaaag catctctaat gactttagct caacgattaa
       61 taacattttt tcattaattt aagttggcta aatgcagtta aaatttttaa cgaccagccc
      121 cccaaaatcg tcgcttccat tttccggagt aataatgaag catgaattat caagtatgaa
      181 ggcatttgtc atactggcag agtccagttc atttaacaat gccgctaaat tactcaatat
      241 tacgcaacct gcattaacgc gaagaataaa aaagatggag gaagatttac atattcaact
      301 atttgagcgt acaactcgca aggttacgtt aacaaaagca ggaaaaaggt tgctcccgga
      361 ggcgcgggaa ttaataaaga aattcgatga aacgcttttt aatattcgtg atatgaatgc
      421 ttatcatcgt ggtatggtga cattagcgtg tattccgacc gcagtatttt attttttacc
      481 gctggcaatc ggtaaattta atgagctgta tcccaatatt aaagtgcgga ttctggaaca
      541 aggcacgaat aattgcatgg aatcggtgct gtgtaacgaa tcagactttg gcataaacat
      601 gaacaacgtc acaaattcat ccattgattt taccccactg gtcaacgagc cattcgtgct
      661 ggcctgtcgg cgtgaccatc cattggccaa aaagtagctt gtagaatggc aggagttagt
      721 gggttacaaa atgattggcg tacgttcctc cagcggcaac cgattgttga tagaacaaca
      781 gctagccgac aagccctgga agctggactg gttttacgaa gtgcgtcatc tttcgacgtc
      841 attaggacta gtggaggcag gactggggat ttcggcgctt cctggtctgg caatgcccca
      901 tgcgccctat tcttcgatta ttggtatccc actggtggaa cccgttatac gtcggacatt
      961 ggggattatt cgccgcaaag atgccgtgct ttctccggca gctgaacgct tttttgcctt
     1021 gctgattaat ttgtggactg acgataaaga caatttgtgg accaatatcg tcgaacgcca
     1081 gagacatgcg ttacaggaga taagttaata caccggcgca ggagagactg cgccggttaa
     1141 atcagcggtt agtgtgcgtt aaccaccacc cacattggtc cctgcccgac cgcatagcgg
     1201 cctttttcat gcagtagccc ctgctcgcca acaatttcgt ataccgagat gtggtgagat
     1261 ttttgcccag cggcaatcag atacttgccg ctgtaatcaa cattgaagcc gcgcggctgg
     1321 gtttccgttg gctggaagcc ttctttactc aacacgctgc catcttccga aacgctgaaa
     1381 acggtaatca gactggcggt acggtcgcag gcgtataaat ggcgaccatc cggggtgata
     1441 tgaatatcag ccgcccaacg ggtgtcggag aagttttccg gcatcatatc cagcgtctgg
     1501 acacattcga tattgccgtg cggatctttc agttcccaga catccactga gctgtttaac
     1561 tcattgacgc aataagcata ttgttcgttt ggatggaata ccatatgacg cgggccggcc
     1621 ccttcaacgg tagtcacttc cgcagggtcc tgcgccacca gatgaccatc atcgctgacc
     1681 gtaaacaggc aaatgcgatc ttgctttaat gccggaaccc acagcgtacg gttgtccggt
     1741 gagatattgg cggaatggca accgtccagc ccctcgacta catcgacgac gcccactggc
     1801 aggccatctt ccagacgcgt tacgctcacg ttacccgcat tgtaagaacc tacaaagaca
     1861 aactgcccct ggtgatcggt ggaaatatgc gtcggactac ccggcagcac agactctgcg
     1921 gcaaaggtca gtgcgccatc gtccggggca atacgatacg ccaggacgcg aaactcaggg
     1981 cgaacaccaa catagagata acgtttgtcc gggctgacca ccatcggctg cacctgcccc
     2041 ggcacatcga caacctgtgt cagcgtcagt gcgccttcat gattcaggtt ccagacgtga
     2101 atttgctggc tctcagggct ggcgatataa actgtttgct tcatgaacac acctcaaaaa
     2161 tgataaatcc cttatctttc atacatataa caacacttgc tggataaaaa acagacaaca
     2221 aaacacgata aaatacaata tcgcacatca attaaatcaa taagttagca ctccatttcg
     2281 aatggcatat tactaaaata ttagtcaccg acacgaattt gacaatgcca cagatatttt
     2341 cgcgactatt cagggagtat gactctcccc gcttttgttt acctgtatca acgaccgtaa
     2401 acactatgcc ataacaactt ctggccaact tcccattcgc tcaacaatca actcacttcc
     2461 ggcacaaata aacgcattac gttgtagatc ggtattctgg tca
//
LOCUS       DADMEQ010000233         2446 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.235, whole genome shotgun sequence.
ACCESSION   DADMEQ010000233 DADMEQ010000000
VERSION     DADMEQ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.235"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            33..1085
                     /locus_tag="JNZ05_004032"
     CDS             33..1085
                     /locus_tag="JNZ05_004032"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HAY8567019.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNSLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMCLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1161..2446
                     /locus_tag="JNZ05_004033"
                     /pseudo
     CDS             1161..2446
                     /locus_tag="JNZ05_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
BASE COUNT          585 a          547 c          627 g          687 t
ORIGIN      
        1 ataagttatg tttttaaatt gagggcatta ttatgaaaaa aataccctgc gtgatgatgc
       61 gaggtggaac ctcgaggggc gcgttcctgt tagcggaaca tttacccgaa gatcaaacgc
      121 agcgcgataa aatattgatg gcaattatgg gttccggtaa cgatctggaa attgacggta
      181 ttggcggcgg taattcactg accagtaaag tcgccattat tagccgttcc agcgatccgc
      241 gtgctgatgt cgattatctg tttgctcaag ttatcgtcca tgagcaacgt gtcgatacca
      301 cgcctaactg cggcaatatg ctgtctggcg taggggcatt cgccattgaa aatggtttga
      361 ttgcagcgac ttcgccagtt acccgtgtac gtatccgcaa cgtcaatacg ggtacgttca
      421 tcgaagctga tgtgcaaacg ccaaatggtg ttgtcgagta cgagggtagc gccagaattg
      481 acggcgtacc gggtactgcc gcaccggttg cgctcacttt cctgaatgcc gctggaacga
      541 aaaccggaaa agttttcccg actgataatc agattgatta ttttgacgat gtcccggtga
      601 cctgtatcga tatggcgatg ccagtcgtca ttattccggc tgaatatctg ggaaaaacag
      661 gttatgaatt accggcggaa ctggatgccg acaaagcatt attagcccgc attgaatcta
      721 tccggctaca agcgggtaaa gcaatgtgct taggtgatgt cagtaatatg gttatcccta
      781 aacctgtgct tatttctcca gcgcagaaag gcggggcaat taatgtgcgt tattttatgc
      841 cgcattcttg ccatcgcgcg ctggcgataa ccggtgctat tgctatttcc agtagttgtg
      901 cattggaagg caccgtcacc cgacaaatcg tcccttctgt aggatatggc aatatcaata
      961 ttgaacaccc cagtggtgcg ctcgacgttc atttaagtaa tgaaggtcag gatgccacga
     1021 cgttacgcgc atctgttatt cggacaacca gaaaaatatt ttccggtgaa gtttatcttc
     1081 cctgaaaaaa ttcgttgtca ggataaggac aatcaataaa ggacttctgt atgagtcata
     1141 cagaaagaac aggattttaa atgaataaga aatcgttatg gaagctaatt ctgatattag
     1201 cgatcccatg tattattggt tttatgccag ctccggcagg attaagcgaa ctggcgtggg
     1261 tgctttttgg tatttacctg gcggccattg tgggtctggt tatcaagcct ttcccggaac
     1321 ctgtcgtact gttaattgcc gttgctgcct caatggtggt ggtcggtaac ttatccgacg
     1381 gtgcgtttaa gaccaccgcc gtattaagcg gttactcttc aggtactacc tggctggtgt
     1441 tctcggcgtt taccttaagc accgcatttg tgaccaccgg tttaggtaaa cgtattgcct
     1501 atctgctgat tggtaaaatc ggtaacacca tgctgggtct gggttacgtt acggtattcc
     1561 tcgatctggt actggctccg gcaacaccgt ctaacaccgc gcgtgcgggc ggcattgtgt
     1621 taccgatcat caacagcgtg gcggtggctt tggggtccga accggaaaaa agtccgcgtc
     1681 gtgtcggaca ttacctgatg atgtccattt acatggtcac caaaaccacc agctatatgt
     1741 tctttaccgc aatggcgggg aacattctgg cgctgaaaat gatcaacgac attctgcacc
     1801 tgcaaattag ctggggtgga tgggcgctgg ccgccggatt gccggattgc cggattgccg
     1861 ggcatcatta tgctgctggt caccccgctg gtaatttaca ccatgtatcc accagaaatt
     1921 aaaaaggtgg ataacaaaac catcgctaaa gcgggccttg ccgaactggg accgatgaaa
     1981 atccgcgaaa aaatgctgct cggtgtcttt gtgctggcgc tactgggctg gattttcagt
     2041 aagtctctgg gggttgatga atccactgtg gcaatcgttg ttatggcgac catgctgctg
     2101 ctgggtatcg ttacctggga agacgtggtt aaaaataaag gcggctggaa taccttaatc
     2161 tggtacggcg gtattatcgg cttaagctcc ttattatcga aagttaaatt tttcgaatgg
     2221 ttagctgaag tctttaaaaa taacctggca tttgatggtc acggtaacgt tgctttcttc
     2281 gttattattt tcctcagcat catcgtgcgt tatttcttcg cttccggtag tgcctatatc
     2341 gttgctatgt tacctgtatt tgccatgctg gcgaacgtct ccggcgcacc gttaatgtta
     2401 accgcgctgg cactgttgtt ctccaactcc tatggcggca tggtta
//
LOCUS       DADMEQ010000234         2439 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.236, whole genome shotgun sequence.
ACCESSION   DADMEQ010000234 DADMEQ010000000
VERSION     DADMEQ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.236"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..565
                     /locus_tag="JNZ05_004034"
     CDS             <1..565
                     /locus_tag="JNZ05_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY8567020.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            523..1200
                     /gene="bglJ"
                     /locus_tag="JNZ05_004035"
     CDS             523..1200
                     /gene="bglJ"
                     /locus_tag="JNZ05_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HAY8567021.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="JNZ05_004036"
     CDS             complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="JNZ05_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HAY8567022.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2167..2403
                     /locus_tag="JNZ05_004037"
     CDS             2167..2403
                     /locus_tag="JNZ05_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HAY8567023.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          627 a          534 c          626 g          652 t
ORIGIN      
        1 agtcattgct gatttagccg gtgaaagtga agatccacgt tctgtttgtg aacattatta
       61 ttctttaatc tcacaatatc gggaaattca ctgggttttc atggtgtcac gctcctggta
      121 ttcccaggct gtagaactgc tcatgtgccc tacggcgacg ttattgtctg atgttgaacc
      181 cattgagaat ctggtcaaga ccgtacgttc cggcaatacg catgcagagc gtatcagcgc
      241 catgctgacc tctccggcaa tgactgaaac tcatgatttt agctatcgct ccgtcattct
      301 cactctttca gagcgcaagg tactgcggct attaggtaaa ggatggggca tcaaccagat
      361 agcttcattg cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg
      421 tcgactggca attcacagca acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc
      481 aagagaactt aacttgcctt ctgtttatgg agatgccgca gaatggaaca cagccgaatt
      541 aagaagagaa atgtcgcact catagaaaaa tgcgtcatga gtagtatcgg tattgagagt
      601 ttattcagaa agtttgcggg caacccttat aagctccata cctataccag tcaggagtca
      661 tttcaggatg ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt ttctgccatg
      721 agaagtgagc gcagagaggg attatcttgc ctgactgagc tggcgattaa gtttccgcgt
      781 acccggcgtt tagttattgt ggatgatgat attgaagctc ggctgattgg ttcattgtcg
      841 ccatcaccgc tggacggtgt attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa
      901 ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa tcagtggtac
      961 attaaccaaa gccggacgtt aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt
     1021 ggctactcaa tgacacaaat tgccgagcag cttaaacgta atatcaaaac gatccgagcg
     1081 cataaattta atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc
     1141 gcagatattc tgttatgtat gcggcattgc gaagcaagta atgtgttgca tccctattaa
     1201 tccgcatgat gccgggttta cttccccggc agtgctttca tttcagcgta caatagccac
     1261 attgctgcac atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccgt
     1321 cgcgcagtac cacggtacgc cagagtggat tatcttcacc gttcgtgagc gttttctcaa
     1381 aaaagagggc atggcgcagc gattcaacag tagcctcgcc gagcagttgc ttcatctcag
     1441 tgagatacca gttgatcaaa taaccggtat tactccagat aagtttgccg ttgatctctc
     1501 cggtcgcttc tagtgcttgc acaaccggaa ccagcgcctg gctgattaac gtttccattc
     1561 gctgctgcgg cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg
     1621 cgacgcgtcc ggtttcgtga aactcagcat ggaaatgttc cggcgacaca tctaatgctt
     1681 tttcctgcgt cagtagcgcc agcattaatg gtggcaccat caggccgata taccattgtg
     1741 cccatagtga gatcagcggt ttgttctcgc ggatcatcgt tggttggttg cgatagatat
     1801 gatcggaata gaccgccagt agagaactta gcgcattcgg tgatgaccat tgcgccagcg
     1861 tcatggcgtt aagcggagcg ggttcatcca ggcggataaa ctccagcaaa tgttcacgat
     1921 gtttggcgat catcgcccgt acggcttgtg caagcccagc atcctgcggc tggagatgcg
     1981 ttcgccagat gacatcttca tagagcggtg cggaacgata ggccataatc gtgatagtaa
     2041 tctaaatgat aatgattgct aatcctatcg ataggtttac tcgatagcaa gggatttctc
     2101 tggcttgcaa atgataaaaa ttatcatatg atattggttt tcattatcaa tgaaagagat
     2161 gaaaccatgt tgcaacgtac gctgggcagt ggctggggag tgttgctgcc gggattgctg
     2221 attgcagggc tgatgtatgc ggatttatcg ccggatcagt ggcggattgt cattctgatg
     2281 ggattagtat tgacgccggt gatgctgtat cacaaacagt tgcggcatta cattttgcta
     2341 ccatcgtgcc tggcgcttat tgctggcatc atgctgatga taatgaattt gaatcaggga
     2401 tgacaaaaca gggaagaaac aagaaaggaa gtaaagata
//
LOCUS       DADMEQ010000235         2405 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.237, whole genome shotgun sequence.
ACCESSION   DADMEQ010000235 DADMEQ010000000
VERSION     DADMEQ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.237"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(339..605)
                     /gene="ospE2"
                     /locus_tag="JNZ05_004038"
     CDS             complement(339..605)
                     /gene="ospE2"
                     /locus_tag="JNZ05_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HAY8567024.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(787..1188)
                     /locus_tag="JNZ05_004039"
                     /pseudo
     CDS             complement(787..1188)
                     /locus_tag="JNZ05_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1617..2405
                     /gene="virF"
                     /locus_tag="JNZ05_004040"
     CDS             1617..2405
                     /gene="virF"
                     /locus_tag="JNZ05_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HAY8567025.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          741 a          426 c          447 g          791 t
ORIGIN      
        1 aagccgtcta tgggaaggag tctgctgtta ttccagagaa tggtaattca gtcatatatt
       61 tctccatcta tgacttgtca ataccatcct gatatttgtg ggtcagcaat atattctact
      121 aaaaggtatt taaggtcatt acgtccctgc actcgaataa tggtgaagaa agcatgataa
      181 atatatcacg ataacgttca gtatgtatcg aggatagatg gagttactcc tctctgaaat
      241 agaattactg ctctactttt tctgtttcca gaaattatta gtaacaaacc ctgatatatt
      301 attttgaaaa tggagcttaa accagtctga aattaacttc agaaatcaga ataccgttgg
      361 ctttgttttt catcaattcc ggcttcattt atctttgtca accattgcat ttctgtagca
      421 gtgaatttca acgttttcgt ccggttggca gcaggtattt gtaattcgta tatagctgct
      481 tttttattga aaacagtata tccatctgta gtaacagtag aagataataa tacgggattt
      541 gatacttcaa ggatgatatt ttcttgtttt tgtggaaggc aagggaatat agtttgtgta
      601 agcatattgc ttccttatgt tatattttag taaagaattt attttttaca ttatagtcgg
      661 ctaaatgaag tttactgtaa aaaaacgata cttttttcgt aagcgatgag atgtggagcc
      721 agaaaaatgg ctgcgttacg tcattgaact gtccttgacc cacgatattc agacagtcgt
      781 tgtatcttaa gttgacatta cccgctgacg tcgatattcc cttggtgaga gataacccaa
      841 cgcactatgc ggatggtttt cattgtaatg ctcgatcgcc actgcaaggt tatgcaatgc
      901 cgttcttggt ttcggcatga acgcgatgca gtcttccttc atcgttttca cgaaccgttc
      961 tgctattcca ttactctgag ggctactgac tgccgtcgtg cagggctcca gattcaactc
     1021 tctggcgaac tgccgtgttt catgagccgt ataagccgaa ccgttatcgg ccactggatt
     1081 ggccgttccg gtcctttatc gccatatcgc tcctgacatg acatcctgca ccgttgcttt
     1141 gtcataatct cccgtgcctg ctgcccagtc tatggcttca cgatcgcaat atggaacgta
     1201 acgctgtcat cgggagaatc gtcaatgcgg cattcggtta acgcttacca atatcaacac
     1261 caaccggatt tgtggagcct ccagtctgaa ggggctttat gcgttccgta taggatatta
     1321 tgatgctgga gttttgcgaa gctccactcc tttccgaaca tttacacaga tattgcctaa
     1381 gaaaagtgaa accataatat ggttatagtc cctttcagtg caaatactta gcttgttgca
     1441 cagagaaata gaagctgcat aagctctttc ttcaaaaaat gtaaataaag ttaaatatag
     1501 gaaaaattac ttaatctatc ttaataacgg aaagattttg tatacaatca ctgttacaga
     1561 aatttcttag ttactctgta aacactaaat atagtttggt atattctgtt gaatttatga
     1621 tggatatggg acataaaaac aaaatagata taaaggttcg cttgcataac tatattattt
     1681 tatatgcaaa aaggtgttca atgacggtta gctcaggcaa tgaaactttg actatcgatg
     1741 aagggcaaat tgcttttata gagcgaaata tacaaataaa cgtctccata aaaaaatctg
     1801 atagcattaa tccatttgag attataagcc ttgacagaaa tttattatta agcattatta
     1861 gaataatgga accaatttat tcatttcaac actcctattc tgaggagaaa agggggttaa
     1921 acaaaaaaat attcctcctc tctgaggagg aggtttctat cgatttgttc aaatctataa
     1981 aagagatgcc tttcggcaaa agaaagatct atagtttagc ttgcctttta tcagctgttt
     2041 ctgatgagga agctttatat acttcgatat cgatagcttc ttctcttagt ttttctgatc
     2101 agataaggaa gattgttgaa aaaaacatcg agaagagatg gcgtctttct gatatttcaa
     2161 ataacttgaa tttatcagaa atagctgtta gaaaacgatt ggagagtgaa aaattaacat
     2221 ttcaacaaat ccttcttgat attcgcatgc atcatgcagc aaagctttta ttgaatagtc
     2281 aaagctatat taatgatgta tcaagactta tcggaatatc aagcccatct tattttataa
     2341 ggaaatttaa tgaatattat ggtataactc caaagaaatt ttacttatat cataaaaaat
     2401 tttaa
//
LOCUS       DADMEQ010000236         2373 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.238, whole genome shotgun sequence.
ACCESSION   DADMEQ010000236 DADMEQ010000000
VERSION     DADMEQ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.238"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..823)
                     /locus_tag="JNZ05_004041"
     CDS             complement(<1..823)
                     /locus_tag="JNZ05_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY8567026.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1424..1672)
                     /locus_tag="JNZ05_004042"
     CDS             complement(1424..1672)
                     /locus_tag="JNZ05_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY8567027.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2027..>2373)
                     /locus_tag="JNZ05_004043"
     CDS             complement(2027..>2373)
                     /locus_tag="JNZ05_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY8567028.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          611 a          439 c          575 g          748 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggttg tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggcgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggagggg gcaatttttc cttaaggtat gaaaaaagga
     1081 gctgaagcta tataatagct attaatgcca ctggtatcag tgaatcatgt atataaaaaa
     1141 ctgtgttaag gaggctgtcc attagcggtt ttttattctt ctggaaccgc ggcatctttg
     1201 cgtatctttt tgtcttctca ccgtctggtc ggtgttttgc tgagactgct aatttcctgt
     1261 ctttgctagt gctgtactgg tactgcccaa tcatgattgg cgggggcggg cggagttgag
     1321 accgcagaca cgtcgtatgc aagaacgtgc tgcggctggc tggtgaactt ccgatagtgc
     1381 gagtattgaa tgatttccag ccgttactga ttttacgtgc taattagtga acaaaccact
     1441 cgtcagcaga ctcccaggta tctttcagag tttcctgaac aaaagtttta gctgaatctt
     1501 tatcggcggt gcgcgtaaca gaaaggccat cgttgctggt ggcttttacg atcacctcta
     1561 catcgtcata acgtttactg atgcgtcggg ttaattcttc ctttaacgca tccacagcac
     1621 cggttggcat tttagtcatt ttctctttgg ctatgcagat ttcaatacgc ataaaaatcc
     1681 ctctacactg tgtttttata cagtattatt tttaactgta tggataaaca gtgtcaagag
     1741 tgagggaaag tgattttttg gggcatgcat ggggcaaaaa tatttgttgt ggggcatttt
     1801 ggggcatgat tgggacactt tgttacatat gaactttgcc gattttcata tgacatggca
     1861 atccaacgct ttgattattc taaagaaaac acatgctctt gggcgttctt tagtgatttt
     1921 taaaaatccc gtatcacgca gttaaagtgg cgggcatact cttcaaggct ggtgatcccc
     1981 aaccgcaccc atttaggatg cgaccattgc ggtagaccga tgtaaatcat aacgcgtgaa
     2041 ggatctcttc cacgctacgc acacgggcga tgcgcgggta gatatgatta atgctgttat
     2101 tgtgttgctc ggcgctggcg gcactacagg cgtcttcggc aatcaccaga ttaaagccaa
     2161 gttcccaggc attgcgggcg gtggattcaa caccgatatt ggtcgagatc ccacataaca
     2221 ctattgtatc gatacctcgg cggcgcagtt gcaactccag atccgtaccg taaaacgcac
     2281 cccactgacg tttgatgatt tcgatatcac tgtcagttgc acctaatgca gctggatgtt
     2341 gccaccagtt ttcgggcaac actttagcgg ggg
//
LOCUS       DADMEQ010000237         2343 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.239, whole genome shotgun sequence.
ACCESSION   DADMEQ010000237 DADMEQ010000000
VERSION     DADMEQ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.239"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            269..406
                     /locus_tag="JNZ05_004044"
     CDS             269..406
                     /locus_tag="JNZ05_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase mbeA domain protein"
                     /protein_id="HAY8567029.1"
                     /translation="MEKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVISFQGKEWVHLK
                     E"
     gene            413..589
                     /gene="mbeD"
                     /locus_tag="JNZ05_004045"
     CDS             413..589
                     /gene="mbeD"
                     /locus_tag="JNZ05_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HAY8567030.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQV"
     gene            complement(948..1415)
                     /locus_tag="JNZ05_004046"
     CDS             complement(948..1415)
                     /locus_tag="JNZ05_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567031.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQQLQIQEVCNSTLWETAKQQLLQESDGRYFAINSKHL
                     DFPGSGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFSHNLT"
     gene            complement(1743..2084)
                     /locus_tag="JNZ05_004047"
     CDS             complement(1743..2084)
                     /locus_tag="JNZ05_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567032.1"
                     /translation="MRRWRFSIGSAPLTSITKIDAQVRGGETRQDYKDTRRFPLEAPS
                     CALLFRPCRLPDTCPPFSLREAWRFLIAHAVGISVRCRSFAPSWAVCTNPPFSPTAAP
                     YPVTIVLSPTR"
BASE COUNT          585 a          493 c          665 g          600 t
ORIGIN      
        1 caaggctgtg acatcaaacg agacgaaaat caaagacgct atagccctgt tcacagcttc
       61 gacagaggaa tcactgaaaa aacaccggga aggggtgaaa gaggcgatga tgcagcacag
      121 gaaggacgtg ttaaagctgg cagggaatac gggcatgatg ttactgggga tggtcatttt
      181 cctgtttacc gtgagtggcg ggacgctctg gtatcttggc gggatgatac aggcgaacct
      241 ggaagaaatc aggaagcaga acgagagtct ggagaagctg aacgcgaaga catggggcgt
      301 ggagtttgtg caggacggga acaggaaatt tctggtcctt ccttacggga agtcagcaga
      361 ggtgatttcc tttcagggga aggaatgggt acatctgaag gagtgacaca gagtgacaga
      421 gctggaaaca catttgctga acgccttaga gcagctgcaa caggactata tgcagcggct
      481 gagcgaatgg gagagcgcct tcgtggaatt gcagaaaatg ttttcgctta cgcaacggga
      541 caacgcgatg ctgaacgagc gggtcatgca gttgagtcag caggtgtagc acttgagcga
      601 gcagacagaa cgcttgagcc agttatacag cgagaactgg agataagaga ggaacggctg
      661 atacaggagc gcgaacatgc gttatccctg gaacgggagc gtcagccgga aatacaggaa
      721 cgcacgctgg atggtccttc gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg
      781 attaggaggg gttaatgtgg cctgtaccat ctggttgcat aggtattcat gcggttaaaa
      841 tttatcaggc gcgatcgcgg cagtttttcg ggtggtttgt tgtcattttt atctgtctgc
      901 tgccgtgatc gcaccaaacg cgtttcagtg gtgcgggcaa taagatatta cgttaaattg
      961 tgagagaatg gtccccatat actgacttta tcatacggat agggttggta gtgggtcgat
     1021 tgggtttcgg cattgtttcc gttaatgcca attgcatttt tgtatttgat gcaacagaag
     1081 gcgtgagatt ctcctgagcc tggaaagtct aagtgtttag aattgatggc aaaatatctt
     1141 ccgtccgatt cttgaagtag ttgttgtttg gctgtctccc aaagggtgct gttacaaact
     1201 tcttgaattt gtagttgttg caggactctt attactgcgt tgtcatgttc caattcttta
     1261 ggggagtgta ttattctctc ttttaataag ctgtttacca caggatttag ttgttgtcta
     1321 aagcacgcag ataatgccat tgctacacaa ttttgttccc gaggaagcat gtttggcggg
     1381 gtatgccgtg tttgatgtga tatgtctatt ctcatagaag gtctctggct tatagcatca
     1441 acaaatacgg ctataaaaca aaattttcat agagtgattc aagatcttct tgagatcttt
     1501 tttggcggtg cgtaatctgc tgcctgcaaa caaaaaaacc accgttatca acggtggttt
     1561 gtttgccgga atctagagct accaactctt tcttccgaag gtaactggct tggtggagca
     1621 cagataccaa atgctgtcct tctagtgtag ccgcagttag gccaccactt caagaactct
     1681 taatatctca atccacctta tccagttacc aatggatgct gccagtggcg ttaagtcgtg
     1741 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1801 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacca
     1861 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1921 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1981 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2041 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcggc gcagcctttc tttgcccttt
     2101 gcctttttgc tcacatgttc tttccggcct tatcccctga ttctgtggat agccgtatta
     2161 ccgtctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgt agcgagtcag
     2221 tgagcgagga agcggaagag cgccggaagc tgcattttct ccttacgcat ctgtgcggca
     2281 tttcacatcg gctatggtgc acttttcata caatccgcac tgatgccgca taagtaagcc
     2341 tgt
//
LOCUS       DADMEQ010000238         2342 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.240, whole genome shotgun sequence.
ACCESSION   DADMEQ010000238 DADMEQ010000000
VERSION     DADMEQ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.240"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..123
                     /locus_tag="JNZ05_004048"
     CDS             <1..123
                     /locus_tag="JNZ05_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567033.1"
                     /translation="PQPKPPAPPAWAMMPPSNSLQLLDETFSVSGTESSATRQH"
     gene            complement(256..768)
                     /locus_tag="JNZ05_004049"
     CDS             complement(256..768)
                     /locus_tag="JNZ05_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY8567034.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            963..1727
                     /locus_tag="JNZ05_004050"
     CDS             963..1727
                     /locus_tag="JNZ05_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567035.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGECDLNDFYSDIQTDAESGFT"
     gene            1678..>2342
                     /locus_tag="JNZ05_004051"
     CDS             1678..>2342
                     /locus_tag="JNZ05_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAY8567036.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          662 a          523 c          592 g          565 t
ORIGIN      
        1 cctcagccga aacctccagc gcctccggca tgggcaatga tgccaccgtc gaactctctc
       61 cagttgctgg acgaaacgtt ctcggtatcc gggacggaat catcagcgac cagacagcat
      121 tgagaatgct tcaggagtac atcaggatcc aatgccttgg ggggtagcgg taattttact
      181 cattatcctt caaatcagat tctgttgtca gaggaatggg ggaagctgga ttcgcaagag
      241 ctttttatgg gcagtttagc taaaaaaagg caacacgaag ctaaaactac caacataaag
      301 tgctgaccag ctagcagctg aaatcagact cactgtatac actttcactg gatgaagttg
      361 caacattcct gccaccaaag gagcaatgta acgcagcacg gcgataaagc gcgaagtgaa
      421 aagtattgac acagagttat tttgcaactg aaggcgaacc cgttctattg tattggaacg
      481 gctcgatatt atacatgcaa gttgagggat atgcccaatt attataccaa gatggtaatt
      541 aacgatagta ccgcaccatg cgccactcat gaccgtgatt ccagcttccc atggtgaaag
      601 agtcgtcttg ctgactgtaa ttacagtggc catcataatt gatgctggtg gcaatatcgc
      661 cgaaatgagt aaagtggatt ttgtgaacgc tattataaaa agaagccccc ataggcgata
      721 tggatgaagt acaaaatagt tcatcaaagc atttatccac tccatcaatg tctttccttc
      781 tgtgaatttt tctttgctat ttaaccgtga tacgacttaa tttaggctta aacaccttac
      841 atttacaagg tactcctgtg gggggccctg ccacggagcg tcgcaggagt gagtgatgag
      901 gaagaattaa agcaacctat tgtgacaggc aacgataaca gtaaatcaca ggaagcataa
      961 tcatggcaaa accggactgg gaggccatcg aatcggcata ccgggccgga gtccttagcc
     1021 tccgtgatat aggcgagaaa tacggcgtta cagaaggggc tatcaggaag agggccaaaa
     1081 agcttggttg ggcacgcagt ggcggtacgc aggtttgcaa aaatggtacg caaaaaagga
     1141 aagtgcgtac cagcagaaag cctgccatta ctggccttac acaaaaaagt acgcaactaa
     1201 aaacagaatc tacaccggat acgaaaccga tacgcggaat gcgtaccgat cccccgacta
     1261 acccattcca acccggtaac cagcaggcat taaaacacgg tggttatgcc cgtcgccttc
     1321 tgctcaaaga tgaggtgata gaggacgcta aagcgttgac gctcgaggac gaattatttc
     1381 gccttcgtgc taacaacctt gtcgctgcag agaatattgg tcggtggttg gtgtcgctgg
     1441 aagatgctaa tggggaccag gaaaggaaga tgctgattga aaatatcagc gccgccgaga
     1501 aagcaatgat gcgcaataca gttcgtattg agtccatcgt tggcacgctt gcgacggtag
     1561 gaaaaatatt tgctgataca gcctaccgca aggccgccac tgataaggtg tctctggagg
     1621 ctgatcgtct tcgccgtgat gcaggtattg atgatggcaa tggagagtgt gacctcaatg
     1681 acttctactc tgacatccaa accgacgctg aatccggctt tacgtagttt ctggactatg
     1741 cgggcacgta acaaagtgct ttatggtggt cggtcatcgt caaaatcatg ggatgccgct
     1801 ggcattgcca tatttctgtc gaataaatac accctgcgtt tttgttgtgc ccgtcagatc
     1861 cagaataaaa tcgaagagtc ggtgtatacc ctgctcaaaa ttcagataga caggtttggt
     1921 ctgcggcacc gtttccgtat tctgaacaac aaaatcatta accgggttac tggctcggaa
     1981 tttgtttttt atggattatg gcgcaacatc gaagaaatta agtcactgga ggggatcgat
     2041 gtgttgtggc tggaagaagc ccacgcactg acggaatacc agtggaaaat tctggagcca
     2101 acgatccgta aagagggttc ggaatgctgg ttcatattca accccggact tgttactgat
     2161 ttcgtctggc gcaacttcgt tgttgatccg cccgaaggca ctctcatccg caaaattaac
     2221 tatgacgaaa atccgtttct gtctgacacc atgcttaagg ttatcgacgc ggcgcgacgc
     2281 cgtgatccgg atggttttaa acatgtgtat gagggcgttc cggagtctga tgatgatgcg
     2341 gc
//
LOCUS       DADMEQ010000239         2301 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.241, whole genome shotgun sequence.
ACCESSION   DADMEQ010000239 DADMEQ010000000
VERSION     DADMEQ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.241"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..784)
                     /locus_tag="JNZ05_004052"
     CDS             complement(<1..784)
                     /locus_tag="JNZ05_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY8567037.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMS"
     gene            1132..2004
                     /gene="rluF"
                     /locus_tag="JNZ05_004053"
     CDS             1132..2004
                     /gene="rluF"
                     /locus_tag="JNZ05_004053"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HAY8567038.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(2137..2301)
                     /locus_tag="JNZ05_004054"
                     /pseudo
     CDS             complement(2137..2301)
                     /locus_tag="JNZ05_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
BASE COUNT          562 a          589 c          576 g          574 t
ORIGIN      
        1 cgctcatatt cgtttcaacc attgcgccgt gaatatcaaa aaagcgcgag caaatatcgc
       61 cagcaacctg gcgggcattc aggtcgtcac tctcttcacc accataaaac gcatgccagt
      121 tagcgccgtc gcgaatggcc ggtgagccaa ttcccaccag ggcgacatcc agattatccc
      181 agtaggcaga gatggtttta aagtgctgag actgcatgat cccattacga attaatgggt
      241 tatccagcag agccggaaaa tccgcgagat gcgattcccc tttcagcttc gctgccgcgc
      301 tgtaggttaa tgtgtttaca tgatagcggc tttcgagttt accggacggt ccgccaataa
      361 ttggtacgca gattaactgc cgcgattgcc ccgcctgcgg caagttttca accagtgcgc
      421 tcaccgcgcg accccaggaa aaaccgacaa tatcgccagg ctccagcaag cgatccagca
      481 gttgcgcgcc gtgtaacccc atcatcgcca gttgagtctc ttcatcctca tcatttcccg
      541 acaccaccac aacgtctttc aggccaaact tttgcttcag ttgctgctcc agccagagat
      601 tttcgttgta gtcatagttg atggcgatgg tgaccattcc ctgatcgcgg cctcgtttaa
      661 gcaagcggct gatggtggtg cggtaaatac ccagttcgcg cgcgatttgc gcctgcgtca
      721 tatcctgttc gtaatagagt tgggcaatct tcacaatcaa acggatatcg tcactgtttt
      781 ccattgccgc ctcctggttc tgcacatttg tgcaaatacg ctttctcacc gcttgatctg
      841 atataacccc atcatgaggg cctttcaaag cgatttttca ctctttgcat ggggagaaat
      901 agcgctttct cttgcacatt tgtttctctg cgtagtagtc atcagcacag gtgtgcagcg
      961 attcgtaata tgttgattat ccttattttg ccaggcaaat ttgcatcgta cttccggaga
     1021 cacttttgtg ataaaaggcg tggcagatca catttaattt cgacgttgcc gcataaccct
     1081 gtttttcccc gctaagaggt acggtatatt ccgcgtttta caggagaatt tatgctgccc
     1141 gactcatcag tccgtttaaa taaatacatc agcgaaagcg gaatttgctc acgccgcgaa
     1201 gcggatcgct atatcgagca aggcaatgtg ttccttaatg gcaagcgagc caccattggc
     1261 gatcaggtga aacccggcga cattgtgaaa gtaaacggtc agttgattga gcctcgggaa
     1321 gccgaagatt tggtacttat cgccctgaac aagcccgtcg gtattgtcag caccaccgaa
     1381 gatggcgagc gcgataacat tgtcgatttc gttaaccaca gcaaacgcgt gttcccgatt
     1441 ggccgcctgg ataaagactc ccaggggctg attttcctca ccaatcacgg cgatctggtg
     1501 aataagatcc tccgtgcggg taacgatcat gagaaagagt atctggtgac ggtcgataaa
     1561 ccgattaccg atgaatttat tcgtggtatg ggggcggggg tgccgatcct cgggacggtg
     1621 acgaaaaagt gcaaagtcaa aaaagaagcg ccgtttgtct tccgcattac cctagtgcag
     1681 gggctgaacc gtcagatccg gcgcatgtgc gagcatttcg gctatgaagt gaaaaagctg
     1741 gaacgtacgc gcattatgaa cgtcagctta agcggcattc cgctggggga gtggcgcgat
     1801 ttaaccgacg atgagttaat cgacctcttt aagctcatcg aaaattcctc ttccgaggca
     1861 aaacctaaag cgaaggccaa accgaaaaca gcgggcatca aacgtccagt cgttaagatg
     1921 gaaaaaacgg cggaaaaagg cggtcgcccg gcgtccaacg gtaagcgttt tacctcgccg
     1981 gggcgtaaaa agaagggacg ctgattaacg cctcagactg atgaaaacgt ccaaaaagag
     2041 ctcggacagt cccctcgccc cttcggggag agggttaggg tgaggggaag attgtgtcca
     2101 cacagacgag tgaaaatctc aggcgttgat tgttcattag cgcctgccgc gcgtcgacgt
     2161 atttgctgac cagctaaatg acgcttcagg atccggttta taagcctgct ttttcttcaa
     2221 ttggcgggct tttttcgctt cagcctcacg ctcgaccatt aatttatcga tgtactctct
     2281 ttcttaatgc tgttggtttc g
//
LOCUS       DADMEQ010000240         2258 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.242, whole genome shotgun sequence.
ACCESSION   DADMEQ010000240 DADMEQ010000000
VERSION     DADMEQ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.242"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(88..>2258)
                     /locus_tag="JNZ05_004055"
     CDS             complement(88..>2258)
                     /locus_tag="JNZ05_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HAY8567039.1"
                     /translation="YHNLDRFEEIFISMDVDDVGREAAREIASRLGEHRCRLVTLPYK
                     DINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAFYGKQQYLFNPPWES
                     LADKFQFREAELTLVNGVNGHGKACPLNEPILLADGTWTTHGNVKIGDQVASVDGNPS
                     TVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEKRRVIDTFGLKRLSE
                     TKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKFSNVEPYMIERMKAE
                     LPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFIPRVFFSANKSTRIG
                     MLCGLLETDGYVEKDGTLVFSSASEELRNGVVQLVNSLGGSCRTRVKTGVTYTYKDDK
                     RHGMDSYEARIRLTREIREAIRSPRLNGRLTAHRFEGCGVFVRNVEKIGNAECLCIMV
                     DHPRHLYVTRGYVATHNTEVVGHMALEAMRQGVKTCIASLELKPGILLKRLTRQATCC
                     KMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFDYARRRYGIQLFIIDSLMKCG
                     IGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDSEEKPTGKMDVKGSGAITDLT
                     DNLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPASVLMLEKQRNGEGWEGGVPLF
                     LDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVTEY"
BASE COUNT          526 a          626 c          486 g          620 t
ORIGIN      
        1 agctatcgtt cacttccctc tcccccaaat aaaaaggcct gcgattacca gcaggcctgc
       61 tattagctca gtgatgtaga tggtcattta atactccgtc acgttttcct gtcgccacgc
      121 ctcgtcatat tccgatttcg gcatattggc gatgtagcta tatggcgatc ctgattcaag
      181 ttgcaggaac tggtgcgatt gctcgtcaag gaacaacggg acaccacctt cccaaccttc
      241 gccgttacgt tgtttttcaa gcatcaaaac agatgccgga gatgccagta gctgttcgtc
      301 cttctctgac atcttttcac cactctgaac tctctgtaac gctctctcgc gagccttgtt
      361 acgccagatg atgaaaaggt tgtctgtcag gtctgttatc gctccagagc cttttacgtc
      421 cattttcccg gttggttttt cttcgctgtc tccttttcgc gagtgagtaa cgagaatgac
      481 gtgggagttt gttttgtttt tgaagtcgca aatcgagtca acaaacgcct tctgcccgtt
      541 atagtcatcg tcgcctatgc cacatttcat caggctgtcg atgatgaata actggatccc
      601 gtatcggcgg cgagcgtagt cgaatatttc gatcagcctg tcggctttcg ccgttccagt
      661 caggccaaac acccaaagtc tttcgtcata aaatttaaat gcagagtcaa tttccagcac
      721 tggcggcatc ttgcagcacg ttgcctgacg ggtaagtcgc ttaaggagaa taccaggctt
      781 cagctcaagt gacgcgatgc acgtcttcac accctgacgc attgcctcaa gtgccatatg
      841 cccgacaacc tcggtgttat gcgtcgccac atatcccctg gttacataca ggtggcgagg
      901 gtgatcgacc ataatacaca agcattctgc attgccgatt ttttcaacat tcctgacgaa
      961 taccccacag ccctcgaatc gatgcgcagt taatctgcca ttgagtcgtg gtgaacggat
     1021 ggcctccctg atttctcttg tcagtctgat tcttgcctcg tatgaatcca tcccatgccg
     1081 cttatcgtcc ttgtatgtgt atgtcacgcc agttttaact cgcgtccggc atgacccgcc
     1141 gagtgagtta accagttgaa caaccccatt gcgcagttct tcacttgctg aggaaaaaac
     1201 aagcgttcca tccttctcga catacccatc cgtttcaagc agaccacaca gcatgcctat
     1261 acgcgttgat ttatttgcgg aaaaaaacac acgagggatg aatttgtttt ttgctgtgca
     1321 ccccattagt ccataacctc gcagggtctc catgagtgga tttacctgac cacgcgccgt
     1381 tgatatcagc cagtccttac catctccaga gaagttgtaa tcaggcagtt cagccttcat
     1441 acgctcgatc atgtatggct cgacgtttga aaacttcacg ctcccattgc taagactacc
     1501 atccccgaga agggagccga taacccatgc taatggctct gagtggtcgc caaagtcacc
     1561 agttatttca ggaatcctaa cgccattttt gtgcctcttc gtttcactca accgcttcaa
     1621 cccgaaggta tctatcacgc ggcgtttctc gcctttcgtg aatccacgac tagtgacctc
     1681 ccataggtga tcgcctgcgc aatcaacata acgaccatct tcaaatgtga ctcggtaaac
     1741 atctctaaca ccctgcggga atatcccagt gacagttgac ggattgccgt ctactgacgc
     1801 cacctgatcg ccaattttta cattcccgtg agtagtccat gtcccatcag ctaaaagaat
     1861 aggctcattc agtgggcatg cctttccgtg accgttcaca ccattgacca gcgtcaactc
     1921 tgcctcacgg aactggaatt tatctgccag agattcccac ggtggattaa acagatactg
     1981 ctgcttgccg tagaaagcgt tgatagtgtc ctggtaaaac tctcgcgcac tgtagagttc
     2041 ttcaggatcg aagtaggatg ccgtgccgat gtactgccag atttcatcct cggtaacacc
     2101 gttcatcagg cattcgttga tgtctttgta cggcagagta acaagacggc aacgatgttc
     2161 accgagtcgg cttgcgattt cccttgcggc ttcacgacca acatcatcaa cgtccatcga
     2221 gatgaatatt tcctcaaacc tgtcgaggtt atgatact
//
LOCUS       DADMEQ010000241         2185 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.243, whole genome shotgun sequence.
ACCESSION   DADMEQ010000241 DADMEQ010000000
VERSION     DADMEQ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.243"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(219..383)
                     /locus_tag="JNZ05_004056"
     CDS             complement(219..383)
                     /locus_tag="JNZ05_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567040.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTQPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1284..1568
                     /locus_tag="JNZ05_004057"
     CDS             1284..1568
                     /locus_tag="JNZ05_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY8567041.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1556..2041
                     /locus_tag="JNZ05_004058"
     CDS             1556..2041
                     /locus_tag="JNZ05_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8567042.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSINHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          566 a          461 c          619 g          539 t
ORIGIN      
        1 caaaatgatg gacattattt ttgtggaacc ggaggtaaca gaccagacgg tttaaatgag
       61 ccggttactt taaacgggtg atggacagca cctcaaaagt tcagatggat tatatttgtc
      121 agcaataccc cgggtatttt cgatttgcga aatggatgga acgacttgct caggggatag
      181 ctgatggagt tactgaagta ccgaaggacc actaaaaatc acaggccggg aatttcggcg
      241 tttccggcgt aggtgtgcca cgaatgcatc cagtggcgtg atgttggatg caatgctgta
      301 tgagagatga ggggctgcgt ccagacgcag ccttcaaacc gccttgagct gctgccgtta
      361 agagcggtgg tggtgagcgt caaggttaaa aggcgcagaa catgtgtcag gtaggtgttg
      421 cggagatgaa cgaaaggaac tgtcgatgaa gtgtcgaaag gaacctctag gcgatgtcga
      481 aaatgtgcta tctacgcagc gcatgacgag tccagcagat atctggtact ggctgggcgg
      541 catccggcat ataggcagca tgaacgcaac acaagctctc gtacggaacg tgggaacctg
      601 tcgtctcgat gctaagggag cggcgcggag cagaggcact gcaagcgcct gagtaccgat
      661 gcgagacaca ggggcagatg gcttcgtagt agtgaggaag tgcctgtaat gggcatggag
      721 cgaagggggt cagtcgttgg ggctggttta tcggtcaacc ggatagccgg gaggagccca
      781 tgagtcagcc aaagccgttc gaggtctcta agtatgcggt ttggaaagca taccagcggg
      841 tcaaagctaa tcgaggtgcg gcaggtgtcg atgggcagtc agtcgaggcg tttgaggtta
      901 ccaatcgaag ctatacccgg taatggcgca ggttgacctg cacttggcca agtggattgc
      961 ccgcaaacac aagcgagcgc gaggtagtct ggtgcgtgca ttcgagtggt tactgcgaat
     1021 tcgtcacgac tgcccgacgc tgtttgcaca ctggtgctta gcttacgata cttgaatggt
     1081 tcaacgacaa gagccgtatg acgcgagagt gtcacgtacg gttccgtggg agcgtagggg
     1141 ggcggtgccc ctgcgccacc cgatatcccc tttgagtgcg gttacgtcca cagaggtaaa
     1201 tgccgctgac gcggcatctg taatctgtta ctttgtgtat atcatttgct atatattgtc
     1261 aatatgctat tgttgtaaag caaatgatat acggaggctt tatgaaatca ggtgttcagc
     1321 tcaatctcag agcaagagaa tcacagcgga tactcattga tgctgctgca gaaattcttc
     1381 ataagtcgcg tacagatttc attctggaaa tggcctgtaa agctgctgaa gatgttattc
     1441 tcgaccggcg ggtgtttaat tttaatgaca ggcaatatga agagttcatt gaaatgcttg
     1501 atgctccggt tgcagatgat cctgccatcg aaaaattgct ggcaaggaaa cctcaatggg
     1561 atgtgtaact gcaccagaac ctttatcctc atttcatcag gtcgctgaat ttgtcagcgg
     1621 tgaagctgta cttgatgact ggttaaagca gaaggggcta aaaaatcagg caatcggagc
     1681 cacccggact tttgttgtat gcagaaaagg tacgcaacaa atagtcggtt tttactcact
     1741 ggccactggt agtatcaatc atacagaagc tacaggtaac cttcggcgca atatgccaga
     1801 ccccattcct gtaattatac ttgcacgcct ggctgtagat gtgtcatttc gaggaaaagg
     1861 gcttggcgcg gatttacttc atgatgcagt tcgtcgttgt tacagggttg ccgaaaatat
     1921 cggtgtgcgt gcaattatgg ttcatgcgct tacggaaaac gcaaaacagt tttatattca
     1981 tcatggtttt aagccatcaa aaactcaggt gcaaacattg tttcttaaac tccctcaata
     2041 gggagtaatg ccgatcagtt aaggatcagt tgaccgatcc agtggctgtg taagaatccg
     2101 gaaacgctca ctcgtttccg gatttttctg tgcacagtaa aagaccacct cctgaggagg
     2161 tggcttttca gaatgccccc tataa
//
LOCUS       DADMEQ010000242         2175 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.244, whole genome shotgun sequence.
ACCESSION   DADMEQ010000242 DADMEQ010000000
VERSION     DADMEQ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.244"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(782..1771)
                     /locus_tag="JNZ05_004059"
     CDS             complement(782..1771)
                     /locus_tag="JNZ05_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567043.1"
                     /translation="MNMTKNAFQFGIEPVHITDNDNLQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(1855..2121)
                     /locus_tag="JNZ05_004060"
     CDS             complement(1855..2121)
                     /locus_tag="JNZ05_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY8567044.1"
                     /translation="MTAYQRRQMNKRAEAERIAKELASQKERTREMFMTEDEWQKELQ
                     RREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWMDSL"
BASE COUNT          570 a          532 c          546 g          527 t
ORIGIN      
        1 cggcggtgcc gtaggcaatg cccgttgaaa ttttgtccat ggatttcata gcctcacctc
       61 cgcaaataac ggatggcgta gttttacact gagaaatgaa agggatttga aaagaaaaaa
      121 acgcaaaagc gggcgaaacg atatatacag taaggaaagc actctatcca acaaaccacc
      181 cacagttaat cggaataaaa gcagagtgct tatgaatgat cgcacgcccg aaggttagta
      241 tttctgcaca gcaattttgc aaaaaaaggc gatcattcat aacttaaacg tctttcagtc
      301 actccgggat ttcccatcat cgcagactga aagactctga ctggagcggg cagcgggagc
      361 ctacgacaaa tcgaagacaa ccagctaacc tattgtttct taaagacagg tgaatccagt
      421 tggttgtagt gatagtcaca catttagaga tagaaggtca agacttgaag cacgtaatat
      481 tccctctaaa ggaatagaac tcacggtctg actacccggc ccttttggtc tggctcaggt
      541 caggctatgg gggcttttgg ctcgctcttt atattagatg aggtctcaca tttttcagtc
      601 aaaactcatc gtctaatagc ccatagccag accaccagtc ccagctttag caggaagacc
      661 acgagataga ctttgtgtag gtccatttgg ccctcctact ttctgtattc acgaactaga
      721 ttactagtac aaaataaagg ccagacatcc agttcggaag tctgaccaat agtcgttatt
      781 gttagttagc ttctttagtg aagctcaggc gtttgactac aggcaggaca gcagtagccg
      841 aaccagtacc catcttgatg gtcgtagcag cttcagctac aacaaacacc ttgccgagaa
      901 tctcaaggtc gtgagactgg gagtctccat cacgtgaaag gaccacagta atgctctcgc
      961 catcgtgacg gaagcaaccg aagctcagag ccgtaaaacg cttagccatc atgaacacgc
     1021 cacggtcttc accgttgtaa taggcgacgt cagtccccag catatcgacg atagcgtctt
     1081 caccaccgaa gccctgctta gcagccagac gggagagagc cttgaggtgt gactcatggg
     1141 cgataatgcc gtagtcagaa acctcagaac cgaaagaaga ctccgtgttc tcgtacaggg
     1201 catccatgat agcgtcagcc ttagcggtca ggctaccagt cagggccgga agttctacgg
     1261 tcttcagcag ctttgaggac tccagcacgt caaccatctt agagttaatg gtacgaatca
     1321 cgttatcgca gatgttcagt gcgtcctgct ggagcgtccc ctgagtgaaa ccagtggtcg
     1381 acttctggtc ccaaagcttt gtttggtgag acacgaggat cgcctgttca atcggctcaa
     1441 cggtgatttc agtgccagac ggagcggtca caacttcagg tcgcttacca ccacggacag
     1501 ggaccagcac agagcggtca gccagagtgt gagcaatgat tggagtcggt aagctgttac
     1561 gggtcggcag gacttctaca gggaacggga tgttatcttg agcgtcttta agtacaccgt
     1621 tcagcatagc cttaacagtc ttagctaact ggagggcata gacctgaggg tcggcgttag
     1681 tcggaaggcc ctcattaacc tgaaggttgt cgttgtcggt gatatgaaca ggctcaatgc
     1741 caaactggaa agcgttttta gtcatattca ttataattaa tctccttgag tcaaatactt
     1801 gaagtcccta acaggcagtc agggaccgtg tgttctcaga tgagggacgc ttgcttacaa
     1861 gctgtccatc cagttaccgc ctgactcaag gtcagcgacg atagcggcac gtgagcgacc
     1921 agcggaatga gtagcgctgc ggaggttttc accataggtc cggcgttcag ctttgacctg
     1981 ttcgcggcgc tgtagctctt tttgccattc gtcttcggtc atgaacattt cgcgggtacg
     2041 ctctttctga gacgctagct ctttagcgat acgctcagct tcagctcgtt tgttcatctg
     2101 gcggcgttgg taggcggtca tcaggtcatt actgaagaag ttaccaatgg tcacgccagc
     2161 ttcagccaga gcttc
//
LOCUS       DADMEQ010000243         2151 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.245, whole genome shotgun sequence.
ACCESSION   DADMEQ010000243 DADMEQ010000000
VERSION     DADMEQ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.245"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="JNZ05_004061"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="JNZ05_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HAY8567045.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="JNZ05_004062"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="JNZ05_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1982)
                     /locus_tag="JNZ05_004063"
                     /pseudo
     CDS             complement(1292..1982)
                     /locus_tag="JNZ05_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1905..>2151
                     /locus_tag="JNZ05_004064"
     CDS             1905..>2151
                     /locus_tag="JNZ05_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567046.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAI"
BASE COUNT          633 a          394 c          491 g          633 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt gagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttat gttgtaattt
      901 atttatggag gttttctatg gaattctttt ttattgtgac tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aatccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgata aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt aaggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt gccgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac
     1501 catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg
     1561 tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca
     1621 gtgttgttgc gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc
     1681 aacatcagcg tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc
     1741 cgtatgacgg tattgatgcc gattttcagt gttctggcgg tataacagcc ccctgcgccg
     1801 ttatgtgcca tttcaacgat ctgctcctta acgccgagtt ttctggcttc ataagagtaa
     1861 gtgagctgga aaacacgttt acatgagcga caacggaaac gctcatgccg ggaacaacta
     1921 cggccatgtc gataaacttc atcggaatta cagcgcggac aatgaacagt aacggtagtc
     1981 atggaaaacc tcaaatacca gtagattacc agaaacttct atcgattgaa gacattacct
     2041 tttgccgcaa taggtggggt aacattggtg aaaacgcatt acgccgggtg gcagttggaa
     2101 aaaaattatc ttttttcggc tctgatcgtg gtggagaaaa tgcggcaata a
//
LOCUS       DADMEQ010000244         2136 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.246, whole genome shotgun sequence.
ACCESSION   DADMEQ010000244 DADMEQ010000000
VERSION     DADMEQ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.246"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            14..487
                     /locus_tag="JNZ05_004065"
     CDS             14..487
                     /locus_tag="JNZ05_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8567047.1"
                     /translation="MRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVADPNLDELYQV
                     YSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGKGLAKKLALMA
                     MEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCEVRMLREL"
     gene            complement(533..949)
                     /gene="rraB"
                     /locus_tag="JNZ05_004066"
     CDS             complement(533..949)
                     /gene="rraB"
                     /locus_tag="JNZ05_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HAY8567048.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1111..2124
                     /gene="argF"
                     /locus_tag="JNZ05_004067"
     CDS             1111..2124
                     /gene="argF"
                     /locus_tag="JNZ05_004067"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAY8567049.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT          544 a          520 c          532 g          540 t
ORIGIN      
        1 gcaatcacct gtaatgcgtc gcctgacgct gcaggataat cctgctatcg ccagcgtcat
       61 ccgtcaggtt tacgcagagt atggtcttac cgccgataaa ggctacaccg ttgccgatcc
      121 gaatctcgac gaactgtatc aagtgtacag ccagcctggc catgcttatt gggtggttga
      181 atacaatggc gaagtggtcg gcggcggcgg gattgcgcca ttaacaggta gtgaagcgga
      241 catttgcgaa ctgcagaaaa tgtactttct tcccgctatt cgcggtaaag ggctagcgaa
      301 aaaactggcc ttaatggcga tggagcaggc tcgagagatg ggcttcaaac gctgctatct
      361 ggaaacgacc gcttttttaa aggaagccat tgcgctttat gagcatttgg gctttgagca
      421 tatcgactat gcgcttggct gcacgggcca tgtcgattgt gaagtgcgga tgctacgcga
      481 actctaaaaa aatgcccgct agtatgtgct atcgggcatt ttaatcaatt aattagtggc
      541 gaaccccgtc atcgtcttca tcgacaaaat cttcatcgtc gccatcttcg ccgttaggat
      601 cttcaaagta agtgccccaa ccgtcgtact caacgtcaaa tttctccgcc agcgtcatca
      661 gttgttcaac ctgggcatcg atcagatcgg cattcaacgc gcactcgctg aggatgtcgc
      721 agcaaatcac gatatcacca tcttcaactt ccagctcttc tggatcggtc acttcgtaac
      781 cgagtttaaa cgcttcaact gccgcttttt ccagagtttc cagatcgtct gcggaaagat
      841 ggtgttcgat ggtgtacagc gcgtccggat cgctgccatc ttccagtaat tcttcaataa
      901 tcaaacgtgt ttcttcacgc tgttcttcca gttgttccgg gtttgccatg gctcattcct
      961 cttaatgtcc tgccgatact cttattgtca cacaccactg acattgcctc cacctttgtg
     1021 acaaagattt atgctttaga cttgcaaatg aataatcata cacataaatt taattttaat
     1081 tcattgaggc gttagccaca ggagggatct atgtccgggt tttatcataa gcatttcctg
     1141 aaattactcg atttcacgcc agctgaactc aacagcctgc tgcagttagc cgcgaagctg
     1201 aaagctgata agaaaagcgg taaagaagaa gccaaactca ctggtaaaaa catcgcgctc
     1261 atcttcgaaa aagactcgac tcgtacccga tgctctttcg aagttgccgc atatgaccag
     1321 ggtgctcgcg ttacttatct tggcccaagc ggtagtcaga ttggtcataa agagtcgatt
     1381 aaagacactg cccgcgtgct tggtcgcatg tatgacggta ttcagtatcg cggctatggt
     1441 caggagattg tcgaaacact ggcggaatac gctggcgtgc cggtatggaa cggcctgacc
     1501 aatgagtttc accccacgca gttgctggcg gatctcctca ccatgcagga gcatttaccc
     1561 ggtaaagcat tcaacgaaat gacgctggtc tatgcaggtg acgcacgtaa caacatgggc
     1621 aattcgatgc tcgaagctgc ggcgcttacc ggtctggatt tgcgtctggt cgcgccacag
     1681 gcatgctggc cagaagctgc gctggttacg gaatgccgcg ccctggcaca acaaaatggc
     1741 gggaatatta cgctgacgga agatgtagct aaaggagttg aaggtgctga ctttatctat
     1801 accgatgtat gggtgtcgat gggtgaagca aaagaaaaat gggctgaacg cattgcatta
     1861 ctgcgtgatt atcaggtgaa cagcaagatg atgcagttga ccggtaaccc ggaggtcaaa
     1921 ttcctccact gcctgcccgc gtttcatgac gaccaaacga cgcttggcaa aaaaatggcg
     1981 gaagagtttg gcctacatgg cggaatggaa gtgactgatg aggtcttcga gtctgccgcc
     2041 agcattgtat ttgatcaggc ggaaaaccgc atgcatacca tcaaagcggt gatggtcgcg
     2101 acgctcagta aattaaataa ttaatatctc tataaa
//
LOCUS       DADMEQ010000245         2128 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.247, whole genome shotgun sequence.
ACCESSION   DADMEQ010000245 DADMEQ010000000
VERSION     DADMEQ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.247"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(246..479)
                     /locus_tag="JNZ05_004068"
                     /pseudo
     CDS             complement(246..479)
                     /locus_tag="JNZ05_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(575..2098)
                     /locus_tag="JNZ05_004069"
     CDS             complement(575..2098)
                     /locus_tag="JNZ05_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY8567050.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          636 a          467 c          451 g          574 t
ORIGIN      
        1 atgacattga tcactttggg gtgactattt tgtttaacaa ataactcttc gacatcaaaa
       61 ttattaatag ttgccatggt aattatttcg gtgatatata aaaacaactg tgataacacc
      121 aaggttgaca actatttgcg caataattcc tttccttcaa tgatccaggc gttcactgcc
      181 agcaacccat tcagccgcat actgacagtg agaaactgaa agattattgc tgatccctgt
      241 ggctgacgag tctttctctt aaatcgatta accagattga tcgccagcca aacaagggca
      301 ttaagcaaat ttatcgtcac aagcacttct cccagcccgg tactgggttt tgcaataagg
      361 atgaccagga gggtgatcag cataaatgca atggaaaaga tgatatagag agtgaaataa
      421 tccagattat tactattgtt catgctacta atggcaatcc tgtcaaaact ttcaaacaat
      481 tatagcaaac aaaataatga acaatagtaa caagaggcaa cgagctaatt agctgtctat
      541 aattacaccg cactatggcc ttactgatca attttcactc aaccacaacc ttcaccttcg
      601 gtttagaaag gataattttg accaggtcga tgtatgtaac aggtttatag aaataataac
      661 cctgctgaaa tgtgatatta ttttggttca gatagccaag ttgttctttc gtttcgacac
      721 cttcagcgac gatactgatc gaaagtttac gcgccagatc caatacacaa tccagaattc
      781 gggttgattc tgggtctgcg tttacgcggc caacgaaact gtgatcaatt ttgatataat
      841 caatatgcag gtcatgaaga taagaaagcc ctgagtagcc agtaccgaaa tcatccagcg
      901 cgatgacaaa accattttca tgcagaatgt tcaaccgctg aaccagactt tcatcaacat
      961 tcaatggctc acgctcggtg acttccagaa caaggtttaa atcgcggcgg gtaaaactgt
     1021 cacggaaatt taaacactcg tcgacaaacg tcggcgaaat aatatgcgaa gcgctaaaat
     1081 taattccaat atgaaaacct tccggcagtt tactcgcgat agcgttcatc tgtctggcaa
     1141 cctgattcat caggctttgt gtaagcggaa cgattaatcc ggatttttca gcaagtggaa
     1201 taaatgccgc gggtgaaata tatccaccgt gaggttgttt ccagcgggct aacacctcaa
     1261 ctccccgcaa tgtcccttcc cgaccattta ccacaggttg gtaaaaaggg acgatctccc
     1321 cattatttat tgcccgtcgc aggatctctt caggcgttgc gcttttattg aaatatctat
     1381 caagcaggaa ggcagcggcg caggcgatta acagaataaa tattaaaatc ccaaagcctt
     1441 tatcgataag tctgtagaag ctaaagagtg gcggcgggtt aaagataata gtgaaatgat
     1501 attttgtgga gtgaaccttc tcggtatagg gataatgtcc attaagcgtc atcacatcac
     1561 ccgttggtcc aataatgtca tccgccacgc gtaatacata ccttacccct ttcaagggta
     1621 cattaagcgc cccgcgaata tgttgatcac ttatcgtaac caaaatgcgc gtatctgcaa
     1681 attggctctg atagagcagg ataggtaatc tattaacggt gtcgattgcc ggagccaaca
     1741 gtaaattact gtccgggaca acaggaatac gagacagcag gacccgattc ccaggcaggg
     1801 atgtacacca gactattccc tgtttgagaa tgatgattgt gcgcaggtga ggtttcagag
     1861 ctgcttcagt gccaagctga tattgctcct cgaggtcgca ttccttcccg gcaatgttaa
     1921 ccgctgtttg agtcgcctgt gacgcttctt caagtatatg attgatattg ttggcggcat
     1981 atcttgctcc cgccaggtac tctgcttttg cggaatacca gagctggatg ttcaaaatag
     2041 cgacaccggt aataaacaag catatcgcaa cgaggatggg tatgagtgta ttgcgcattt
     2101 gcttgtcccg gccaaagggt ttaataca
//
LOCUS       DADMEQ010000246         2118 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.248, whole genome shotgun sequence.
ACCESSION   DADMEQ010000246 DADMEQ010000000
VERSION     DADMEQ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.248"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            129..1166
                     /gene="curA"
                     /locus_tag="JNZ05_004070"
     CDS             129..1166
                     /gene="curA"
                     /locus_tag="JNZ05_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HAY8567051.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVMGVAGGAEKCRHATEVLGFDVCLDYHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1364..2029
                     /locus_tag="JNZ05_004071"
     CDS             1364..2029
                     /locus_tag="JNZ05_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY8567052.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          583 a          453 c          567 g          515 t
ORIGIN      
        1 ggcaaatcca acggattcct cgatgaaatt attgcgtgtt gcagagggga tgtgacggct
       61 gcaaacaaaa ccaatcccgt gagcctgcta ctattgatgg attcatgcca aaagcacagg
      121 agagcattat ggggcaacaa aagcagcgta atcgacgttg ggttctggcc tcgcgtccac
      181 atggcgcacc ggttccggag aatttccgtc ttgaagaaga tgatgtcgcc acaccgggtg
      241 aaggacaggt gttactgcgc acagtttatt tgtccctgga cccgtatatg cgtggacgta
      301 tgagcgatga gccatcttat tcaccgccgg ttgatattgg cggcgtgatg gtcggcggta
      361 cggtgagccg tgtcgtggag tcgaatcatc ctgattatca gcctggcgac tgggtgctgg
      421 gctacagtgg atggcaggac tatgacatat ccagtggtga tgacctggtg aaacttggcg
      481 atcatccgca aaatccatcg tggtcgctgg gtgtgctggg gatgccaggc tttaccgctt
      541 atatggggct gctggatatc ggtcagccta aagagggcga aacgttggtg gtagctgcgg
      601 cgacaggacc agtgggggcg acggtggggc aaatcggcaa acttaaaggt tgcagagtga
      661 tgggggtagc cggtggcgcg gaaaaatgcc gccatgctac cgaggtgtta ggcttcgatg
      721 tttgccttga ttaccatgcg gacgattttg ccgaacaact ggcgaaagcg tgcccaaaag
      781 gtattgatat ctattatgaa aacgtgggcg gcaaggtatt tgatgcagtg ctaccattac
      841 ttaatacatc tgcgcgcatt cccgtttgtg gcttagtgag cagctataac gctacagagc
      901 taccacccgg tccggatcgt ttacccctgt tgatggcaac ggtgctgaaa aaacgtattc
      961 gcttgcaagg gtttattatc gctcaggatt atggtcaccg catccatgag tttcagaagg
     1021 agatggggca atgggtgaaa gaggataaaa tccactaccg cgaagatatt actgatggtt
     1081 tagagaatgc gccacagacg tttatcggcc tgctgaaggg taaaaacttc ggcaaagtgg
     1141 tgatccgcgt cgcgggtgat gattaaagtt tcaacggcgg cgtaagccgc cataaaaatc
     1201 caggcaaaaa gcttattttc agctttaatt aactaaccac gtcattgttc tgctgctaat
     1261 aacaacaaag gtgggtggtt tatgattatt cactttaata cactaggtga actccttctg
     1321 ccatgcaggc agggttggac agaaaacacg cgaaggaatt aaaatgcctg gaacgggaaa
     1381 aatgaaacat gtcagtttga ctctgcaggt tgagaacgac ctgaaacatc agcttagtat
     1441 tggcgcatta aaacctggcg cacgcctgat tactaaaaat ctggcggagc aattaggtat
     1501 gagtattaca cctgtgcgtg aagcattatt acgtctggtt tcggtgaatg cgctttctgt
     1561 cgcacctgca caagcattta cagtaccgga agtgaggcaa agtcagttag acgaaatcaa
     1621 tcggatccgc tacgaactgg aattaatggc agttgccctg gctgttgaaa acctctcctc
     1681 gcaagacata gcagaacttc aggaattgct cgggaaatta cagcaggcgc aagaaaaggg
     1741 cgacatggaa caaatcatta atgcgaacag gctatttcgc ttagcgattt atcatcgctc
     1801 aaatatgccc atcctgtgtg agatgattga gcaactgtgg atcaggatgg gacctgcttt
     1861 acattatctt tatgaagcga ttaacccagc ggaattacgg gagcgcatag aaaactatca
     1921 tctattactc gctgcgttaa aagcaaaaga caaagaggga tgcaaacatt gtcttgctga
     1981 aattatgcaa caaaatattg ctattttata ccagcaatac aatcgttaaa ttaaatgccc
     2041 ggcaaatacc gggcatcaag taattactca aatcgccacg caatattcat gccgacacca
     2101 taatttcgtc cgggcgca
//
LOCUS       DADMEQ010000247         2117 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.249, whole genome shotgun sequence.
ACCESSION   DADMEQ010000247 DADMEQ010000000
VERSION     DADMEQ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.249"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..256)
                     /locus_tag="JNZ05_004072"
     CDS             complement(<1..256)
                     /locus_tag="JNZ05_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567053.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSM"
     gene            281..520
                     /gene="relB"
                     /locus_tag="JNZ05_004073"
     CDS             281..520
                     /gene="relB"
                     /locus_tag="JNZ05_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HAY8567054.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            520..807
                     /gene="relE"
                     /locus_tag="JNZ05_004074"
     CDS             520..807
                     /gene="relE"
                     /locus_tag="JNZ05_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HAY8567055.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            879..1034
                     /gene="hokD"
                     /locus_tag="JNZ05_004075"
     CDS             879..1034
                     /gene="hokD"
                     /locus_tag="JNZ05_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HAY8567056.1"
                     /translation="MKQQKAMLIALIVIYLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1252..1502
                     /locus_tag="JNZ05_004076"
                     /pseudo
     CDS             1252..1502
                     /locus_tag="JNZ05_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1569..1847
                     /locus_tag="JNZ05_004077"
     CDS             1569..1847
                     /locus_tag="JNZ05_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567057.1"
                     /translation="MARNAKYYNHDNSTVLAHTHERYSHAFKSDWYQHPSCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSEQPPRPSRTFQQRIWR"
     gene            1849..>2117
                     /locus_tag="JNZ05_004078"
     CDS             1849..>2117
                     /locus_tag="JNZ05_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567058.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRLLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNAQVIRAAGGAGALSDWLLRH"
BASE COUNT          551 a          482 c          549 g          535 t
ORIGIN      
        1 acatgctaaa gcaaaaaata gatgaataag ttgagttgtg catatgtagc ctgaccgtca
       61 caaagtatat ggtgtctgta ccagtaagat gatggccgga ctctttaaaa aagagctgac
      121 ctgcacaata caggatggac ttagcaatgg ctgctcctgg cacaaagcgg acagtgatca
      181 ccgttcttac gactactttc tgacttcctt cgtgacttgc cctaagcatg ttgtagtgcg
      241 atacttgtaa tgacatttgt aattacaaga ggtgtaagac atgggtagca ttaacctgcg
      301 tattgacgat gaacttaaag cgcgttctta cgccgcgctt gaaaaaatgg gtgtaactcc
      361 ttctgaagcg cttcgtctca tgctcgagta tatcgctgac aatgaacgct tgccgttcaa
      421 acagacactc ctgagtgatg aagatgctga acttgtggag atagtgaaag aacggcttcg
      481 taatcctaag ccagtacgtg tgacgctgga tgaactctga tggcgtattt tctggatttt
      541 gacgagcggg cactaaagga atggcgaaag ctgggctcga cggtacgtga acagttgaaa
      601 aagaagctgg ttgaagtact tgagtcaccc cggattgaag caaacaagct ccgtggtatg
      661 cctgattgtt acaagattaa gctccggtct tcaggctatc gccttgtata ccaggttata
      721 gacgagaaag ttgtcgtttt cgtgatttct gttgggaaaa gagaacgctc ggaagtatat
      781 agcgaggcgg tcaaacgcat tctctgaacc aaagcatgac atctctgttt cgcaccgaag
      841 gtgacacttc tgctttgcgt tgacaggaga agcaggctat gaagcagcaa aaggcgatgt
      901 taatcgccct gatcgtcatc tatttaaccg tcatagtgac ggcactggta acgaggaaag
      961 acctctgcga ggtacgaatc cgaaccggcc agacggaggt cgctgtcttc acagcttacg
     1021 aacctgagga gtaagagacc cggcgagtga gaaatccctc gccacctctg atgtggcagg
     1081 catcctcaac gcacctgcac ttaacccgct tcggcgggtt tttgttttta ttttcaacgc
     1141 atttgaagtt ctggatggtg ccggaataga atcaaaaata cttaagtagc gcgcagggat
     1201 aaaagggatg gaccccgaac aggggagtgc tacttatctg gaaggattct gatgatgaac
     1261 atcgaagaac tgcgtaaaat tttttgtgaa gatggcctct atgctgtgtg cgttgaaaat
     1321 ggaaatattg ttagtcatta ccgcattgtg tgtttgcaaa aaatggggct gcgttaatta
     1381 attttgtgga tgcccgagtg acggacggat ttatcttgcg cgacggtgag tttgtcactt
     1441 cattacaggc attgaaagag accggaataa aagctggctt ttctgctttt tcagaagaat
     1501 aaactcatct acaatcttgc gcggggctga actcccgctg agtaacaccg tgccaccgga
     1561 gaaaaccgat ggcacgcaac gcaaaatatt acaatcatga taattcgacc gttcttgccc
     1621 acacgcacga gcggtattct cacgcattta agtcagactg gtaccagcat ccctcatgca
     1681 ctgaagaaca ggccgaatgg ctcattcagt gttaccgcag gcgcggatgc gaggttaaaa
     1741 aagcccttag cctcgactac cgtcactgga taatctccgt caggctccct tactccgaac
     1801 agccaccgcg tccgtcccgc acattccagc aacggatctg gaggtaacgt gcgggtatta
     1861 cttcgacctg ttctggttcc ggaactcggg ctggtggtcc ttaagcccgg tcgtgaatcc
     1921 atgccggtat tccacaatac ccggttactg gtggagccgg aaccgaaaag catgcgtaat
     1981 ctgccgtccg gggtcgttcc tgccgttcgc cagccgctgg tggaagacaa aacattgctg
     2041 ccgtttttca gtaacgcaca ggtgattcgt gctgctggtg gtgctggtgc attgtctgac
     2101 tggctgttgc gccatat
//
LOCUS       DADMEQ010000248         2099 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.250, whole genome shotgun sequence.
ACCESSION   DADMEQ010000248 DADMEQ010000000
VERSION     DADMEQ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.250"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            1..799
                     /locus_tag="JNZ05_004079"
                     /pseudo
     CDS             1..799
                     /locus_tag="JNZ05_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1238..2075
                     /locus_tag="JNZ05_004080"
                     /pseudo
     CDS             1238..2075
                     /locus_tag="JNZ05_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          517 a          507 c          638 g          437 t
ORIGIN      
        1 tctgggcctt gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa
       61 atacgccacg atacgtaaga tgctgacaga tatctttaac agtaattacc ggtgttatgg
      121 ctatcgtcgc ctgcatgcga tgctcaggca tgaggggggt cggctatcag aaaaggttgt
      181 acgcagactt atggtggagg aacagcttgt cgtcagtcgt aaccgtcgtc gtcgctacag
      241 ctcatattgc ggagaaatcg gaccggctcc ggataacctt atcgccagag attttaaggc
      301 ggagcaacct aatcagaaat agctgacaga tatcacggag ttccagctcc ctgcaggtaa
      361 agtctggctg tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag
      421 tacacgccct gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa
      481 tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg
      541 gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc
      601 tgataatgct gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg
      661 taaatggtcg ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg
      721 gtataacgag cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca
      781 acaatgcggg ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat
      841 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa
      901 ctctcatgct gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag
      961 gcagacaccg gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac
     1021 ggacttcgat atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta
     1081 cagtgtgccg gaaagcctgt gtggtcagct ggtatcaata cggatctcgc tggatgatga
     1141 actgcggatc tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata
     1201 cggctggcag accgtgccgg agcatcacgc tccactctgg cagcaggctt cggagcgcat
     1261 ggccgaacgg ctgggggaga tacagaaacg ggtaatcact gtctgtgacc gggaagcgga
     1321 tatctggcat tacctgtatt ataaagtcag ccacggacag cgtggtgcgt gctgcacaga
     1381 atcgccggct ggaagaggca cccggcaagc tcttcgaact gccggaagtc ctggcaaccg
     1441 caggaagcca cacgctgaat gtgatgcaaa aaggcgggcg ggcagcccgt caggcccgga
     1501 tgttcatcag ttacagcgaa gtcagcataa aaaatcccga caacagcggc caggcgctcc
     1561 cgctcacgta tgtctgttgc cgggagcagg cagaggacgg tgcctgctgg catctgctga
     1621 cgtcaggaaa agcggcgagt gccgccgatg cacgacgtat tgtcagccat tacgagcgac
     1681 gctggctgac agaggaatac cacaaggcgt ggaaaagtgg tggtacatgg aatcgctgcg
     1741 aatgcagacc cgggataacc ttgagcgcat ggtggttatc caggcgttta tagcggtacg
     1801 ggtgctgggc ctgcggcagg gaggtgtcag tgaagaaaca caaaatgaca gctgtgagaa
     1861 aatactgacg ccgacagagt ggaaactact gtgggttaag ctggaaggaa agccgcttcc
     1921 ggtgcaggca ccgaccctga aatgggcctg gggcgatggc atgacagcaa acgcacaggt
     1981 cgtcccggtt ggggcgtcat gtgggatggc tggtcagact tcaggatatg gttgagggtt
     2041 acctggtgat gaagtctctt gatcaggagc tctgatcaag agacagcagc ttagggggg
//
LOCUS       DADMEQ010000249         2087 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.251, whole genome shotgun sequence.
ACCESSION   DADMEQ010000249 DADMEQ010000000
VERSION     DADMEQ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.251"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(60..1025)
                     /locus_tag="JNZ05_004081"
     CDS             complement(60..1025)
                     /locus_tag="JNZ05_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAY8567059.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVGLWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1877..>2087)
                     /locus_tag="JNZ05_004082"
     CDS             complement(1877..>2087)
                     /locus_tag="JNZ05_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567060.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          575 a          549 c          436 g          527 t
ORIGIN      
        1 aaaaaaaata aactaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cgacgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagccct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataaccg caattttaac ccccaaaaca agcacccccg aacggctacc ccaacctttt
     1381 ccacgcaccc gatcaccaaa acagggaata aaaacaaaac cctgtcttac aggtggatgg
     1441 cctcacttcg tgaaaccatc caccccgcat aagttttttc atcggtggtg tcgtcaatca
     1501 ctaaatcgga accccaaagg ggacccccac gatttagcat ttgacgagat taactctcgg
     1561 agatggcatc atgcttttaa cggactttaa aaaacggtga acggatgcgg attttaatcc
     1621 gtgtctgtga ggcgttgagc aacgagcaaa gcgaggctgt agaaagagag ccagcggcag
     1681 gctcaggacg ataatccggg gaaaatgtac cactcgcaga gtgttaacat ttgacatcgg
     1741 attgtctatc ctgcattggt gtcgagttgg caaggctgag gataatgctg aggataatgc
     1801 aggatagaca gagcactaga gattgcatgc aatctcgtac ctatcacctc agcctgccgc
     1861 tggctctttt ccagaactaa gaatgcgttc ttaaaaatcc aaacttgata ccgtttaaat
     1921 ctattttcac aaaaagagtt tttaccctct cgtttgtttt ctgaatatat aaaattaatt
     1981 catcttcaaa accttcgtgc ggatgaaaac caaatgtgcc aaaacttaat ccatcaataa
     2041 gcaaaataag gtttttaaac ctgtcaggaa tcactgcttc acttctt
//
LOCUS       DADMEQ010000250         2066 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.252, whole genome shotgun sequence.
ACCESSION   DADMEQ010000250 DADMEQ010000000
VERSION     DADMEQ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.252"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..690
                     /locus_tag="JNZ05_004083"
     CDS             <1..690
                     /locus_tag="JNZ05_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8567061.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLLGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     EMFGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAIYLLAGCLISAM
                     TALLMKDNQRS"
     gene            792..>2066
                     /gene="amn"
                     /locus_tag="JNZ05_004084"
     CDS             792..>2066
                     /gene="amn"
                     /locus_tag="JNZ05_004084"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY8567062.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLC"
BASE COUNT          484 a          511 c          496 g          575 t
ORIGIN      
        1 ctgggagcat tgtgggtgcg caatggcatg gaggagtccg cggaatttga acaacagcaa
       61 cataatcaag cggccgcgaa aaaacgcatc ccggttatcg aagctctgtt acgacatccc
      121 ggtgctttcc tgaagattat tgcactacga ctgtgcgagt tgctgacgat gtacattgtt
      181 actgcctttg cacttaatta ttcaacccag aatatggggt taccgcgcga acttttcctt
      241 aatattggtt tgctgttagg tggattaagc tgcctgacaa ttccctgttt tgcctggctt
      301 gccgatcgtt ttggtcggcg cagggtttat atcacaggcg cgttgatcgg aacgttgagc
      361 gcatttcctt tctttatggc gcttgaagca caatctattt tctggatagt tttcttctcc
      421 ataatgctgg caaacattgc gcatgacatg gtggtgtgtg tgcaacaacc gatgtttacc
      481 gaaatgtttg gtgccagtta tcgctacagt ggtgctggag tcggttatca ggttgccagt
      541 gtggttggcg gtggatttac accttttatt gccgctgcac tcatcactta ctttgccggg
      601 aactggcata gcatcgccat ttatttgctg gctggatgcc tgatttctgc aatgaccgct
      661 ttattgatga aagacaatca acgctcgtga tagcctgccg aagatcatcc gatcttcgcc
      721 ttatactttt gtttcacatt cctgtgacat actatcggat gtgcggtaat tgtatggaac
      781 aggagacaca catgaataat aagggctccg gtctgacccc agctcaggca ctggataaac
      841 tcgacgcgct gtatgagcaa tctgtagttg ctttacgcaa cgccattggc aaatatatta
      901 caattggcga attacctgat gaaaacgccc gtaaacaagg tctttttgtc tatccatcac
      961 tgaccgtaac ctgggacggt agcacaacaa atccccccaa aacgcgcgca tttggtcgtt
     1021 ttactcacgc aggcagctac accaccacga ttactcgccc tactctcttt cgttcgtatc
     1081 tgaatgagca actaacgttg ctttatcagg attatggtgc gcatatctct gtgcaaccct
     1141 cgcaacatga aatcccttat ccttatgtca tcgatggctc tgaattgaca cttgatcgct
     1201 caatgagcgc tgatttaact cgctacttcc cgacaacaga actggcgcaa atcggcgatg
     1261 aaactgcaga cggcatttat catccaacgg aattctcccc gctatcgcat tttgatgcgc
     1321 gccgcgtcga tttttccctc gcacggttgc gccattatac cggtacgcca gtagaacatt
     1381 ttcaaccgtt cgtcttgttt accaactaca cacgttatgt ggatgaattc gttcgttggg
     1441 gatgcagcca gatcctcgat cctgatagtc cctacattgc cctttcttgt gctggcggga
     1501 actggatcac tgccgaaacc gaagcgccag aagaagccat ttccgacctt gcatggaaaa
     1561 aacaccagat gccagcatgg catttaatta ccgctgatgg tcagggtatt actctggtga
     1621 atattggcgt gggaccgtcc aatgctaaaa ccatctgcga tcatctggca gtgctacgcc
     1681 cggatgtctg gttgatgatt ggtcactgtg gcggattacg tgaaagccag gccattggag
     1741 attatgtcct tgcacacgct tatttacgcg atgaccacgt tcttgatgcg gttctgccgc
     1801 ccgatatacc tattccgagc attgctgaag tgcaacgtgc gctttatgac gcaaccaagt
     1861 tagtcagcgg caggcctggt gaggaagtca aacagcggct gcgtacaggt actgtggtaa
     1921 ccacagatga caggaactgg gagttacgtt actcagcttc tgcacttcgt tttaacttaa
     1981 gccgggccgt agcaattgat atggaaagtg caaccattgc cgcgcaagga tatcgtttcc
     2041 gcgtgccata cgggacacta ctgtgt
//
LOCUS       DADMEQ010000251         2062 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.253, whole genome shotgun sequence.
ACCESSION   DADMEQ010000251 DADMEQ010000000
VERSION     DADMEQ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.253"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..123
                     /locus_tag="JNZ05_004085"
     CDS             <1..123
                     /locus_tag="JNZ05_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8567063.1"
                     /translation="CFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            complement(272..1726)
                     /gene="ospC2"
                     /locus_tag="JNZ05_004086"
     CDS             complement(272..1726)
                     /gene="ospC2"
                     /locus_tag="JNZ05_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HAY8567064.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFINNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          646 a          368 c          337 g          711 t
ORIGIN      
        1 tgctttgggc ggctgaaaga atatcgccgc atcgccacgc gttacgacaa aacggcgaga
       61 aattacctgg cgatggtgaa actgggctgc atccgactct tttatcaacg cttacgtaat
      121 taagggacac agcctagtct ccaggattcc cggggcgttc accacatgca atgtgttgtt
      181 gcaggggaac ggtctgcacc atcccctata tcgatcaata taacaaaaat gacaacgtca
      241 gattcagggc acaacctaaa tatattttag gtcacctccc aaatcgtttg cctgataccg
      301 ctccattttt taataaaaaa tcaattgttt ttgaatcttt gcttttcatt gcattgtcaa
      361 gcatagtgtc cccactattt gctttttgaa atcttttgtt tacatcaacc aatccattat
      421 taataaaata ttctagcact ttatagttgg cactatcttc actaagtaaa tactcaacat
      481 catacagctc acaatacata ttgttgttca ttttctccat ctctgcgaca tcttgttttg
      541 taaaattaaa tttagatagt aaataaagtg ccatatcaga ttttgcattg cctagagaat
      601 aagcaagggc ctgatgagcc atttttttat cagtcacctt attgttaatt tcttcataat
      661 tggatgcttt aatggcatcc tccaaagata tatctctgag tcttatcttt ttgaagttat
      721 ctgtgcttag cattcttggt atatgatact caagctgaaa cacaaagttt ataattctat
      781 ccagactaac aggatcaatg tttttattat aacaaaactc tcggaatctt tgatctttac
      841 tctttcttat aaaatcaata agatgcaacc caattccaag cctcatatct tttgtattaa
      901 atattggcac atcagttttt ccctgattac catgcacata cctgctaact tcatccacaa
      961 cctctttaaa gttatctaag aaaaatgatt ggtgctcatg gtaaaaaaca ggtggaatag
     1021 cattatcaaa gtggtcggtt aatgtcatat atccatatgg agagtcatga tcaatgatat
     1081 acgcattagc accaaaatcc acagtatgat gtgttttatt tagtgggagt gtttcttgat
     1141 gattggaaat ttcaacccca aaaaaaacaa aatcgtggtt agctatttct tcaatatcct
     1201 cagagaatgt gtttttatat gtttgtatat tctcagactt aattttatca aaggacttaa
     1261 tattaagagt attatttgac aggttagcgt tactttgatg tctgaagtta aaatcagaat
     1321 taataataat agagagcaag tgacgttcat ccgaagaaag aggatgagtt ttttgtatta
     1381 tttcagaaag atagtgtctg gacaccttat cgaggtgctc aagtgaccga aatgatgacc
     1441 gtgctccaga tggcgcttca atagaaagat ttaatgattt gaaactggtc ccttgagaga
     1501 acattttact ataactttgc gctgctatag acttcctcaa aaagcttttc acagtatcag
     1561 cacagtgatc taaatgtttt tcacttatgc cagaagagga tgagtttgtt acgcatgata
     1621 tgtttttttg taatgctttt gtatctttgt tcggatttat ttttccatca acaagatcaa
     1681 tattgttctg aacattaata tgatttactg cttcaggtat tttcataaaa catcccccat
     1741 tatgtgcgtt tttatctgca gttatttaga ataatattat ctaacttaat attttcttgt
     1801 atgtattata acgaacatat tttaatatgt tgtacgattt tcactctgaa atcccctgag
     1861 tttaatcaca cttcctttac tgaaaggaat tattgcaaca gtattatttg tttttggata
     1921 tatatcttgc gccttgttat gtagttaatg ctcagaatat gatagataaa atagagacgc
     1981 aaaacagggc atgctcataa catgatgata acataaaaaa ttagtaacaa taaatgaaag
     2041 aatcatgggc gctcaacaaa aa
//
LOCUS       DADMEQ010000252         2059 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.254, whole genome shotgun sequence.
ACCESSION   DADMEQ010000252 DADMEQ010000000
VERSION     DADMEQ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.254"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(89..1501)
                     /gene="pykF"
                     /locus_tag="JNZ05_004087"
     CDS             complement(89..1501)
                     /gene="pykF"
                     /locus_tag="JNZ05_004087"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HAY8567065.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIQII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          522 a          458 c          497 g          582 t
ORIGIN      
        1 tgatttttta ttgcttctgg ttatcgatta aataaaaaaa gcgcccatca gggcgcttcg
       61 atatacaaat taattcacaa aagcaatatt acaggacgtg aacagatgcg gtgttagtag
      121 tgccgctcgg taccagtgca ccagaaacca taactacgac gtcacctttg tgtgccagac
      181 cgctctgcag agccagttct ttacccagac ggtagaaatc atcagtagaa gtgatctctt
      241 taaccagctg cggcacaacg cctttgctca gtaccaactg atgagccgtt ttttcgttgg
      301 tggtcagtgc caggatggtg gcatccggga agtatttacg tactgcgcga gcagatttac
      361 cgccctgggt agcaaccacg atcagcggag catccagttt ttcagcagtt tcaacggcac
      421 cacggcatac cgcttcggtg atgcgcagtt tacggttgtc attgttgaac tcgagacggc
      481 tgttcatcac gcggtcggta cgttcgcaga tggtcgccat gatagaaacc gcttccagcg
      541 ggtatttacc ttttgcggat tcaccagaca gcatcactgc gtcagtaccg tcgaggatgg
      601 cgtttgcaac gtcaccggct tctgcgcgag tcgggcgtgg gtttttgatc atggaatcca
      661 gcatctgggt cgcagtgata acgactttac gtgcacggat acatttttcg atcatcatct
      721 tctgggcgaa gataacttct tctaccggga tttctacacc caggtcgcca cgcgcaacca
      781 tgatgccgtc agaggcttcg aggatttcgt cgaagttgtt gaggccttcc tggttttcga
      841 ttttggagat gatctggatg ttttcgccgc cgtgcgcttt caggtgctca cggatttcga
      901 taacgtcaga acgcttacga ataaaggaag cagcaacaaa gtctacgcct ttttcgcaac
      961 caaagatcag gtcctgtttg tctttttcag ccagtgctgg cagagcaatg gaaacgccag
     1021 gcaggttcac acctttgttt tcgcccaggt caccgttgtt cagcacttta cagataactt
     1081 tgttaccttc aatggcggta acttccatac cgatcagacc atcgtcaacc agtacggtgt
     1141 tgccaacaga caggtcagta gtgaaacctt catacgttac cgcaaccatt tcgctgttgc
     1201 cgataacaga tttatcagtg gtgaaagtaa aggtctgacc agctttcaga gaaacatcgt
     1261 taccgccttc cagtttcatg gtgcggattt ccggaccttt ggtatcaagc aggatagcgg
     1321 cggttttacc agttttgctc atcacgttgc gcagattctg aatgcgctga ccgtgttctg
     1381 catagtcacc atgagagaag ttcagacgca taacgttcat gccggcgtcc agcattttag
     1441 ctaacatctc ttcagattcg gttttcggtc cgatggtgca aacaattttg gtctttttca
     1501 tgacagtctt agtctttaag ttgagaagga tgggagaaac ttgctttctg ggcgcactgc
     1561 cggaaagcga atccggtatt acgaaagtgg tggtgcctcg ctctaaggat aggtgacatc
     1621 gaaaaagcgt gcagaggaat gtgtgcttgt gtctcagccc aacgtgagtg cctgtttttg
     1681 agtcgttgag ttctacagtc caaagtgctg aaaccattca agagtcaatt ggcgcgcatt
     1741 atacgctgaa attaatccaa aaggaaaaca aaaacagcgt ttcaaaaaat atacaacttg
     1801 ttagacaata aattgttatc aaagatttaa cgttccaggg aaaaggttac gcaaaaactt
     1861 cgctgtttgc gcaacgtctg gtgccgaagt tgtcagtgtg aggataagtg aaagttcatc
     1921 cggtgtataa atatatcatc gctcaattgc tacagttaaa ataatttatc gcgttatttg
     1981 tctgattttg tttgattttt gtttaattgg ttagattgat atatgctgat agttggcatg
     2041 ataaataggt ataaagaat
//
LOCUS       DADMEQ010000253         2040 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.255, whole genome shotgun sequence.
ACCESSION   DADMEQ010000253 DADMEQ010000000
VERSION     DADMEQ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.255"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(36..305)
                     /locus_tag="JNZ05_004088"
     CDS             complement(36..305)
                     /locus_tag="JNZ05_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY8567066.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            362..1030
                     /locus_tag="JNZ05_004089"
     CDS             362..1030
                     /locus_tag="JNZ05_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY8567067.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1346..>2040
                     /locus_tag="JNZ05_004090"
     CDS             1346..>2040
                     /locus_tag="JNZ05_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY8567068.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPEFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT          616 a          445 c          379 g          600 t
ORIGIN      
        1 ctgcatacgt cgtgtctgcg gtttttcttt ttcgcttaca ttgtgtctgg ttcttccggc
       61 cactcaatat caggtgcagt tgatgtatca acacggttca gcaacacccg atacttcttc
      121 caggcttcca gcaacgagtt ttcttcctcc gttgcgatct ccagatctac agcatcctga
      181 agtggcgcaa tatgctcact gaatccctgg atgtagaact gtgtggtgac ggtcttccag
      241 ccattcggct cctgctgtat cgaagcatac caggctattt caatatcgct atgctgcggc
      301 agcatttaac cccttgtaat tcatcgccat aattgattta attcacaaat aaaactataa
      361 tatggtgaaa ttaatgaaaa aaaacacaga tgatggggct aaaatttaca caccacttac
      421 cctaaagctt tatgactggt gggttttggg agtatcaaat cggcttgcat ggggatgtcc
      481 tacaaaggaa caccttcttc cacactttct ggaacattta ggtaacaacc atctggatat
      541 tggtgttgga actgggtttt accttactca cgtacctgag agtagtctga tatctttaat
      601 ggatttgaac gaagctagcc tgaacgcggc atctacaagg gctggggaat caaaaattaa
      661 acataaaatt agccatgatg tttttgaacc ttatcctgcg gcgttacatg gtcaatttga
      721 ttccatttcc atgttttacc ttcttcactg cctgcctgga aatatatcta caaaaagctg
      781 tgtaatacgc aatgcggcgc aggccttaac tgatgatgga actctatacg gagccacaat
      841 tcttggcgat ggagttgtgc acaatagctt cggtcaaaaa ctgatgcgca tttacaatca
      901 gaaaggcatc ttttcaaaca caaaagattc cgaagaaggc ttaacacata tactctcaga
      961 gcatttcgag aatgttaaaa ccaaggttca aggtactgta gtaatgtttt ccgcttcagg
     1021 gaaaaaatag catccaaccg cagcacgttc ttgcttaaga cgtgctacgg tttcctttac
     1081 gatttggggt tgcgacttta cctctattga taatgcattc cggcagaacg ttcaaatatg
     1141 aacgtacgat atttaactaa ccgaaaaaca aaatatagca caggcgagac atttaccatc
     1201 taagaaaata gtatcgtttt tttacagtta aatcaacatc acttccttaa aatgaaaaca
     1261 ataaatttca atcctgaaac attttatttc agcttattat tccccagtat cacattttat
     1321 caggatatcc ttctgcgagg ttattatgct tcctgtaaat aatccccccc tatccactgg
     1381 aaacgtctct ttttacagaa ctacatcaat cgacaatgtt cacaataatt atctctccga
     1441 atgggttgaa tggactaaaa acagcatttc cggagaaaac agggaaactg cttttacccg
     1501 gctccaatta tgtctggaga acagtgaaac atcgttggac ttatcttgtt taggtctcag
     1561 atctctacca cgattgcctg acaatcttga tgaaattaat gtaagcaata accaactatc
     1621 aatgctcccc gagctaccaa gggcattgaa agagctgaat gcaagcagta atcaattatc
     1681 tgcacttcct gaattaccag tgtcgctgga atatataaat gtgagtgata accatttgtt
     1741 cgcacttcct gaattacctg cgtcactaga atatattaat gtaagtgaca atcacctgtc
     1801 tgtacttccg aggttaccaa tgtcattgga attacttgat gcagccagaa atgctttgga
     1861 agtaatacca gagtttccag aaagagatga tcatattata agaatattct ggcttaatca
     1921 gaaccggatc acggcaattc cggaaagcat acttggcctc agttctgata gcgttgtcaa
     1981 tcttagagaa aatcaactat ctcccagaat aatgcaaact ttgttacaac aaaccgccca
//
LOCUS       DADMEQ010000254         2032 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.256, whole genome shotgun sequence.
ACCESSION   DADMEQ010000254 DADMEQ010000000
VERSION     DADMEQ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.256"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..362)
                     /locus_tag="JNZ05_004091"
     CDS             complement(<1..362)
                     /locus_tag="JNZ05_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002443793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8567069.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /locus_tag="JNZ05_004092"
     CDS             complement(429..1643)
                     /locus_tag="JNZ05_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HAY8567070.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVMMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          634 a          492 c          412 g          494 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt gcgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcat gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgactactac catacaagag agaaaaagcg attattcaac cgtgttcaca
     1741 ttaaaaacaa aaatttccac cgaggagaaa aaataccctc aaaaaccacc tgaataatta
     1801 accattattg ttaaaacaac acgttaaata atcacataac aaactccatt aatcattgaa
     1861 aatgagcaca gaaatagaaa ttaattatat tcaattaatt aaaatgtgta ttgcttgatg
     1921 tatttaaaaa tattattttc atggtgagag tggaggtaaa gaagacacaa taataaaaag
     1981 gaatgtgaca aataatggtt ctggttgggc gttaaattta aggtcaccag aa
//
LOCUS       DADMEQ010000255         2029 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.257, whole genome shotgun sequence.
ACCESSION   DADMEQ010000255 DADMEQ010000000
VERSION     DADMEQ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.257"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..709)
                     /locus_tag="JNZ05_004093"
     CDS             complement(<1..709)
                     /locus_tag="JNZ05_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY8567071.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPLSLPMELTPSCNEFYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQN"
     gene            complement(1098..1364)
                     /gene="ospE1"
                     /locus_tag="JNZ05_004094"
     CDS             complement(1098..1364)
                     /gene="ospE1"
                     /locus_tag="JNZ05_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HAY8567072.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(1473..1696)
                     /locus_tag="JNZ05_004095"
                     /pseudo
     CDS             complement(1473..1696)
                     /locus_tag="JNZ05_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          581 a          347 c          425 g          676 t
ORIGIN      
        1 cattctgagc taatctcagg acactttccg gaagtgttgt cagattatta ttcattagca
       61 cagccctgtt catactaaaa ggtaattccg gtagttgttc tataaaatta ttagccagag
      121 ccagcccttc aagcttatct ggaaaggggg gaatgctgtc taatctgttt ccagaaacta
      181 ccagtaattt tagttccggt ggcaatagtg gcagaatatg caatcggtta ttatgaaccc
      241 gtaaatgagt tagattttca ggaagaaatg gtaattcttt tatcttgtta tcacccgcat
      301 taagctcttc taacgtctcg ggtaaggcag gtaagctttc cagttgatta aattgagcat
      361 gtattacctt aagcattggt ggtaaatcag ggagatgtgt taataggttt tttcttattt
      421 ccagtgttgt tatctgaggc ggtaaatctg gtaatgtttt taaatctaac tcagataaat
      481 ttaattctgc ctcttgattt tgtaaacata ttttcagcct attgaaggcg atattgcgtt
      541 gctctcctgg ggtaccgttc ttttcccatt cgctccagac ttttaaataa aattcattac
      601 aagatggagt cagttccata ggtaaagata atggagttaa cgagtatata ttgtttattt
      661 gatgcataat gccagaaccg attgcctgta tattggttga tttaatcatt ataaccagaa
      721 ttcttatcaa cgataagaat tctagtgaaa tagaatatgg cttattaatt ttaaaacagt
      781 ttgctttgta ccctttctcc gggtattgct ttccccctaa gccgtctatg ggaaggagtc
      841 tgctgttatt cctgagaatg gtaattcagc cataaaaagg tatttaaggt cattgcgtct
      901 ctccctatac tcgaataatg gtgaagaaag catgatgaat atatcacgat aacgttcagt
      961 acgtatcgag gatagatgga gttactcctc tctgaaatag aattactgct ctattttttt
     1021 ctgttttcag aagttattag taacaaaccc tgatatattt ttttgaaaat ggagcttaaa
     1081 cgagtctgaa attaacttca gaaatcagaa taccgttggc tttgtttttc atcaattccg
     1141 gcttcattta tctttgtcaa ccattgcatt tctgtagcag tgaatttcaa cgttttcgtc
     1201 cggctggcag caggtatttg taattcgtat atagctgctt ttttattgaa aacagtatat
     1261 ccatctgtag taacagtaga agataataat acaggatttg atacttcaag gatgatattt
     1321 tcttgttttt gtggaaggca agggaatata gtttgtgtaa gcatattact tccttatgtt
     1381 atattttggt aaaaaattta tttttttaca ttatagtcgg ctaaatgaag tttactgtaa
     1441 aaaaacgata ctttttcggg tggcgcttcc aatcagtaga atatgtgttt ttcaatgaag
     1501 gagccgatga cttttcgtgg atttccacgg aacgcgagaa gcagattgtt ttacgtgcca
     1561 gccgcttgat gcgggtgcgg agggtcaggt tgttacgttc aagatatagc atgtgctgga
     1621 tgaagatccc ttttctggtc acctgtttat ctttcgtggc cgacggggtg acactgttaa
     1681 aatgtaatga acgcatccca cacaattaaa cacggagtag tatgatggtt aaaagggtga
     1741 aactggcagg tgtggagcct cgcagctttc cggcatcata ctgcccaata cgggatgcat
     1801 aaagcgtcct cagactggag actccacaaa tgaaatatac accggttggc gttgatatgg
     1861 caaaacatgt cattcagatt cacttcatca atgagcacat gtcagcgcca gtgatataag
     1921 acggtaatgg ggatgcggac gatttcctgg actctcagaa gatgaaacat gtttactgaa
     1981 gaacaaaaaa ttcgcgctat tgagctttat ttcaaatatg gtaagaagc
//
LOCUS       DADMEQ010000256         1977 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.258, whole genome shotgun sequence.
ACCESSION   DADMEQ010000256 DADMEQ010000000
VERSION     DADMEQ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.258"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JNZ05_004096"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JNZ05_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HAY8567073.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
     gene            1494..>1977
                     /locus_tag="JNZ05_004097"
     CDS             1494..>1977
                     /locus_tag="JNZ05_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HAY8567074.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     V"
BASE COUNT          563 a          341 c          477 g          596 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg
     1261 tcagggatta aaagatgaac gtaaatttat tcaacgcatt aattttaaag ggttttattg
     1321 tttgttgacg aaaacaggaa tcatgttcgg tctcttttta tctgttaaaa gccagaagca
     1381 tttccttcgc tgactttata gtcaaccata acacacactc tactgtctga gtccagcgtt
     1441 ttttaacatt cttgttaaga ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac
     1501 agcctgcgcc cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg
     1561 gcgatataca cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc
     1621 cgtggcgaga tttacttatc tcagtgggag acgttatcga tcgtgggccg caaagtttac
     1681 gttgtctgca gttactggaa caacattggg ttcgtgcggt aagaggcaat catgaacaga
     1741 tggcgatgga tgcgctggca ttccagcaga tgtctttgtg gttgatgaat ggcggcgact
     1801 ggtttattgc gctggcagat aatcaacaga aacaagcgaa aacggcgctg gaaaaatgtc
     1861 agcatttgcc ctttattctt gaagtacaca gtcgcaccgg caagcatgtt attgctcatg
     1921 ccgattatcc agatgatgtt tatgaatggc aaaaggacgt tgatttgcat caggtct
//
LOCUS       DADMEQ010000257         1931 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.259, whole genome shotgun sequence.
ACCESSION   DADMEQ010000257 DADMEQ010000000
VERSION     DADMEQ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.259"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(821..1060)
                     /locus_tag="JNZ05_004098"
     CDS             complement(821..1060)
                     /locus_tag="JNZ05_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY8567075.1"
                     /translation="MPQGGVISPLLSNIILNEFDQYLNKRYLSGKARKDRWYWNHSIQ
                     RGRSTAVKENWQWKPAVAYCCYADDCVPRRRVLGT"
     gene            complement(1255..1612)
                     /locus_tag="JNZ05_004099"
                     /pseudo
     CDS             complement(1255..1612)
                     /locus_tag="JNZ05_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1623..1931)
                     /locus_tag="JNZ05_004100"
                     /pseudo
     CDS             complement(1623..1931)
                     /locus_tag="JNZ05_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          427 a          538 c          466 g          500 t
ORIGIN      
        1 tcgtcaaaaa aatcatcttc gtgaagtttt acgacatagc ggaggagttt gcttctttga
       61 ggtgcaggtt ttcgtttgcg tggtttagct gaacgggcca tataactacc tgaaagacaa
      121 tgatattgtc tgtttttata acggtaatca caggccatga caagccgcag ccgtcaggcc
      181 gcctacttgg ggcggtttag aacgcttatt tagtaccaga tcttacttat aataaagggg
      241 cttggcagtt tggataaact attgtcctta ttccactaat acaaactttt tgtaaagtat
      301 attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa caattacacc
      361 ataaaaggaa gagggggccc caattaagtt gtcaagcatg tttactgcgc aatcgcttgg
      421 tgctccgtcc ctgtaccccg cattggtact ctggcgctca tggggcttcc acttgcgctt
      481 ctcccttcac atcggggtga caggtccacg ttccccacaa gagcctgaat cgacttcacg
      541 ccgcttttat gccggatgcc gcgcaggcag taaacatatc ccacaggaac gactccacca
      601 ttgttttgac atcatctata tgctttcgac acatcatcag cggttcactt tcgttcgtct
      661 cttcgaacca tacttgatcg gctcacgtcc gaccttttcc catctcgctc accaccacat
      721 ctcttaaatg cagcagcagt ggatggtttg gtgcctgcgc ctgcacgccg acaccgaggg
      781 gccttccctc atctcttgtg ctgcatggct gcacagggac tcacgtcccc aggacgcgcc
      841 ttcgtggcac acagtcatcg gcatagcaac agtaagcaac ggcaggtttc cattgccagt
      901 tttctttgac agcagtactt cggcctcgct ggatactgtg attccagtac cagcgatcct
      961 ttctggcttt tccactcagg tagcgtttat tcagatactg gtcgaactca ttcagtatga
     1021 tgttcgacag caacggcgag atcacaccgc cctgtggcat actttcactg accgcccgga
     1081 agagaccaac atcaacacgg cgttttccac agcagtctca taaaacgtgc atcactgatc
     1141 ctgcggcaga cggctttcat cagcagccgg tgatgtactg tgtcgaagta gctggacagg
     1201 tcaccttcaa taacccggcg ccccgtgttt ccccgcagtc ggtgagctgt aatagtacgt
     1261 gatgtctccc gcccacttct ggttcgggcc actggcgtaa aaatcctgcg tcagcagatt
     1321 ttctgataca ggcaggccgt gtgcacggta actgatcggg ctgaacttct gggaggcttt
     1381 tgcgctcagt tcctgacggt gcagactggc tgccacggtt tttacgttaa agtggtaacc
     1441 agagcacaca gttcatccgt caggcgtggg gcaccgtaac gctggactgg gtaaaagcct
     1501 tgcggacaac gctgtcgcag tgctgacgga actgctgacg tgtgtttatc cttgtccgcc
     1561 gctgacacca cgtataccag ccgctgcggg ccacccggag tacgcggcac attgccttga
     1621 tgacggttta cgcggtcgcc tgcggcaacg gctttccggc tgacggtagt gtggaccttt
     1681 ttaccccgga gaacaccggc aagccccata accgccatga gacgtgccac agtgcatctg
     1741 gccactctga taccttcccg taacaactga tgccagactt tacgcacagc gtatacctga
     1801 tgattctcat cgtatacgcg cagtgtctct ttcttcagcc agtcatcgcg ctgcgcacgg
     1861 gcactgcgtt tatcatgatg atgtcgctgt tgctgacagt ggtaatacgt tgacggggca
     1921 atatgcagtt c
//
LOCUS       DADMEQ010000258         1888 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.260, whole genome shotgun sequence.
ACCESSION   DADMEQ010000258 DADMEQ010000000
VERSION     DADMEQ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.260"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..65)
                     /locus_tag="JNZ05_004101"
     CDS             complement(<1..65)
                     /locus_tag="JNZ05_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8567076.1"
                     /translation="MMMELQHQRLMALAGQLQLESL"
     gene            complement(62..418)
                     /locus_tag="JNZ05_004102"
                     /pseudo
     CDS             complement(62..418)
                     /locus_tag="JNZ05_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            498..1691
                     /locus_tag="JNZ05_004103"
     CDS             498..1691
                     /locus_tag="JNZ05_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HAY8567077.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(1717..1888)
                     /locus_tag="JNZ05_004104"
                     /pseudo
     CDS             complement(1717..1888)
                     /locus_tag="JNZ05_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          513 a          433 c          466 g          476 t
ORIGIN      
        1 aggctttcca gttgcaactg cccggcgagc gccatcagtc gttgatgttg cagttccatc
       61 atcacgccac tcctctgcag aatgagtcgt agatggagag tggatgatgc tggggggggt
      121 tgtcgaagct caccagattt tcaccaggat gcacgtcata ctcttttttc tccggaggca
      181 gtgccagcat ggactgctgc tcttcgagcc agcgatcaca gggacgggcc tggattgttt
      241 catgctttcg ttggttagcg acatcgtgca gccagcgcag accgtggcgg ctggctgttt
      301 caacatcgac agtgctcccc atcgggcgca ggcgagtcat tagtgggatg taaaaactgt
      361 tacgggtgta ctgcaccatc cgttccacct tacctttagt ctgtgccctg aagggctgag
      421 gaatccccag aaaacgagac aaacatagca ttatgtgatc tactgatttg tgccaacaat
      481 tcaatgagat cactgctatg cctgttcata aagtatgtca gaatttcttc cgggatgcct
      541 tagcaccatt tcatcagtac cgacaaaatg ccctgatgga tgccacaatg gcgttaatca
      601 acggagcatc tctgacgcag accagtatcg gacgtttttt gccgggtaat gcacaagtta
      661 agaataagat caaacgtatt gatcgcctca tgggtaatga ggcgctccat cgcgatattc
      721 caatgatttt caggaatatc acttcaatgc tgacacggca gttatcttta tgtgtaatag
      781 ccgtagactg gagtggctat ccctcccagg agcatcatgt tcttcgtgcc agccttctgt
      841 gtgacgggcg ttccattccg ttattgagta aggttgttcc ttctgaaaaa caaaataatc
      901 cattgataca acatgatttc cttgattccc ttgctcaatc cctgcccccc gatgccaggg
      961 ttattattgt cacggatgct ggatttcaga gcgcatggtt tcaccatatt acttctctgg
     1021 gctgggattt tattggtcgg atcagaaata acgttcagta ttgcctcgat aacgcgcctg
     1081 aaagatggtt aaaagtgtcg gatagccctg aatgtaaaac accggaatat atgggggctg
     1141 gaaggctggt taaagaaagg aaaaaaagca tccgggggca tttttacacc tataagaaat
     1201 cggcaaaagg gcgaaaaaag aaacgttcga aaggtcagag tggtttaaac aaaacagaca
     1261 aagaacagag caaatcagca aaagaagcct ggttgatctt cagcagtaca aatgacttca
     1321 gagcgcgaga aatcatcaag ttatacagtc ggcgaatgca gatagagcaa aactttcgtg
     1381 atgaaaagaa cgggcgcttc ggatttggtc ttcgggccag caaaagtcgt tcaacaggaa
     1441 gaattctggt tctgagtctg ctggcaacgt tgagcactat cgtaatgtgg ttactcggtt
     1501 atcacgctga aaataaaggg ttacacctga aatatcaggc taacagcatt aaatcccggc
     1561 gggttatctc atatctgacg ttagcgaaga atgttcttcg acactcgcca cttattttaa
     1621 gacgaacagt actgagcaca gttctaaacc atctgtccag aacctaccga aatatggtgc
     1681 tggtttatta gcagtaattt tgtggggatc cctcagcctg aaggggcgac acagtcgggg
     1741 agagaagccc atctccttgc cgaactgcca cagcgaagga tggaaccggt gctgaccggt
     1801 ctgatatgcg tcacgttgca gaaccacagt tttcatattg tcatacaaca cttcgcgcgg
     1861 cacaccacca aagaagcgga acgcatta
//
LOCUS       DADMEQ010000259         1869 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.261, whole genome shotgun sequence.
ACCESSION   DADMEQ010000259 DADMEQ010000000
VERSION     DADMEQ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.261"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            27..1869
                     /gene="cyoB"
                     /locus_tag="JNZ05_004105"
                     /pseudo
     CDS             27..1869
                     /gene="cyoB"
                     /locus_tag="JNZ05_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
BASE COUNT          357 a          486 c          504 g          522 t
ORIGIN      
        1 attaaagggg ttgaggaaga ataaagatgt tcggaaaatt atcacttgat gcagtcccgt
       61 tccatgaacc tatcgtcatg gttacgatcg ctggcattat tttgggaggt ctggcgctcg
      121 ttggcctgat cacttacttc ggtaagtgga cctacctgtg gaaagagtgg ctgacctccg
      181 tcgaccataa acgcctcggt atcatgtata tcatcgtggc gattgtgatg ttgctgcgtg
      241 gttttgctga cgccattatg atgcgtagcc agcaggctct tgcctcggcg ggtgaagcgg
      301 gcttcctgcc acctcaccac tacgatcaga tcttcaccgc gcacggcgtg attatgatct
      361 tcttcgtggc gatgcctttc gttatcggtc tgatgaacct ggtggttccg ctgcagatcg
      421 gcgcgcgtga cgttgcgttc ccgttcctca acaacttaag cttctggttt actgttgttg
      481 gcgtgattct ggttaacgtt tctctcggcg tgggcgaatt tgcgcagacc ggctggctgg
      541 cctatccacc gctatcggga atagagtaca gtccgggagt cggtgttgat tactggatat
      601 ggagtctcca gctatccggt ataggtacga cgcttaccgg tatcaacttc ttcgttacca
      661 ttctgaagat gcgcgcaccg ggcatgacca tgttcaagat gccagtattt acctaggcat
      721 cactgtgcac gaacgtactg attattgctt ccttcccaat tctgacggtt accgtcgcgt
      781 tgttgaccct ggatcgctat ctgggcaccc atttctttac caacgatatg ggtggcaaca
      841 tgatgatgta catcaacctg atttgggcct ggggccaccc ggaagtttac atcctgatcc
      901 tgcctgtttt cggtgtgttc tccgaaattg cggcaacctt ctcgcgtaaa cgtctgtttg
      961 gttatacctc gctggtatgg gcaaccgtct gcatcaccgt gctgtcgttc atcgtttggc
     1021 tgcaccactt ctttacgatg ggggcgggcg cgaacgtaaa cgccttcttt ggtatcacca
     1081 caatgattat cgccatcccg accggggtga agatcttcaa ctggctgttc accatgtatc
     1141 agggccgcat cgtgttccat tctgcgatgc tgtggactat cggttttatc gtcaccttct
     1201 cggtgggcgg tatgactggc gtgctgctgg cagtaccggg cgcggacttc gttctgcata
     1261 acagcctgtt cctgattgcg cacttccata acgtgatcat cggcggcgtg gtcttcggct
     1321 gcttcgcagg gatgacctac tggtggccta aagcgttcgg tttcaaactg aacgaaacct
     1381 ggggtaaacg cgcgttctgg ttctggatca tcggcttctt cgttgccttt atgccgctgt
     1441 atgcgctggg cttcatgggc atgacccgtc gtttgagcca gcagattgac ccgcagttcc
     1501 acaccatgct gatgattgca gccagcggtg cagtactgat tgcgctgggt attctctgcc
     1561 tcgttattca gatgtacgtt tctattcgcg accgcgacca gaaccgtgac ctgactggcg
     1621 acccgtgggg tggccgtacg ctggagtggg caacctcttc cccgcctccg ttctataact
     1681 ttgccgtagt gccgcacgtt cacgaacgtg atgcattctg ggaaatgaaa gagaaaggcg
     1741 aagcgtataa aaagcctgac cactatgaag aaattcatat gccgaaaaac agcggtgcag
     1801 gtatcgtcat tgcagctttc tccaccatct tcggtttcgc catgatctgg catatctggt
     1861 ggctggcga
//
LOCUS       DADMEQ010000260         1782 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.262, whole genome shotgun sequence.
ACCESSION   DADMEQ010000260 DADMEQ010000000
VERSION     DADMEQ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.262"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..452
                     /locus_tag="JNZ05_004106"
     CDS             <1..452
                     /locus_tag="JNZ05_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8567078.1"
                     /translation="VGWNWNSGVYDANISGASTLILHFNKNTGSCPAVQFRVNYKNGG
                     IFYRSARDGYGFEAGWSEFYTTTRKPSARDVGAYTQAECNSRFITGIRLGGLSSVRTW
                     NGPGWSDRSGYVVTGSVNSNRDELIDTTQARPIQYCINGRWYNAGSI"
     gene            455..988
                     /locus_tag="JNZ05_004107"
     CDS             455..988
                     /locus_tag="JNZ05_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY8567079.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGIVI
                     KRTYTGEEQRQQVENEKQSLLQLVRDKTQLWDSQLRLGIISAGNKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1017..1544)
                     /locus_tag="JNZ05_004108"
     CDS             complement(1017..1544)
                     /locus_tag="JNZ05_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY8567080.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKNFDVIWLWTEDGKNWYGEVKNF
                     QPDTIKIVYDENNIIVAITKDASTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERSVRLNMATDEECARLESWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(1546..1782)
                     /locus_tag="JNZ05_004109"
                     /pseudo
     CDS             complement(1546..1782)
                     /locus_tag="JNZ05_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435977.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          498 a          391 c          400 g          493 t
ORIGIN      
        1 ctgttggctg gaactggaat agcggtgttt atgatgcaaa tatcagcggt gcatcaacat
       61 taatcctcca cttcaataag aatacgggga gctgccctgc tgtacagttc cgtgtgaatt
      121 ataagaacgg cggtattttt tatcgttcag cgcgtgatgg ttatggattt gaggctggct
      181 ggtcagagtt ttacaccaca acacgcaagc catcagcgag agatgttggc gcatatacgc
      241 aggcagaatg taactcaagg tttattacag gtattcgcct tggtggtctg tcatctgttc
      301 ggacatggaa tggtcccggc tggtctgaca ggtcaggtta tgttgttacc ggttccgtta
      361 actcaaacag agatgaacta attgatacaa ctcaggcaag gccaattcag tattgcatta
      421 atgggaggtg gtataacgcg gggagtattt aacgatgatg cacttaaaaa acatttctgc
      481 tggtaatcct gaaacaaaag agcaatacca gctaacgaaa caatttaaca tcaaatggct
      541 ttatacagag gatgggaaaa actggtatga ggaacaaaag aactttcagc ctgatacgtt
      601 gaaaatggtc tatgaccaca acggcgttat tatttgtatt gaaaaggatg tttcagcaat
      661 taatccggaa ggcgcaagcg tcgttgaggt tcctgatatt acagcaaatc gccgggctga
      721 tatttcaggt aaatggatgt tcaaagatgg catagtgata aagcgaactt ataccgggga
      781 agagcagagg caacaagtgg aaaatgaaaa gcaaagcctg ttgcaacttg tcagggataa
      841 aacccagcta tgggactcac agctacgact gggcatcatt tccgccggga ataagcagaa
      901 attaaccgag tggatgctct acgcgcagaa ggtcgaatcc acagacacct ccagtctgcc
      961 agtaacgttt cccgaacaac cagaatgaga gaaggcccgc tatcgggcct taatttttac
     1021 tctggttttt gtggccattc tggctttgcc gtatccacac ggctgaccag aacactgtag
     1081 cgttcccatg actccagtcg tgcgcattcc tcatctgttg ccatattcag cctgacagag
     1141 cgctccagcg gctggatgac tgattcagct tcggaaagca atgcggcttt ttgtgattcg
     1201 gcctgttgct gctgttcgtc tgccgtataa atccgcttaa ccacagctcc gtccttaaac
     1261 atccacttcc cggaatcatc ggcacgacga tttgctgtta tatcggggac ctcaacgacg
     1321 ctaaagcctt caggattaag cgtggaggca tctttagtga tggcgacaat aatattattt
     1381 tcatcgtaaa caatctttat tgtatctggc tgaaagttct tcacttcccc ataccagttt
     1441 tttccgtctt ctgtccacaa ccagattacg tcaaaattct ttgttaattc gtactgttcc
     1501 agtgttttag cgttacccgc tttaatgttc tttaagtgca tcatattaaa cgctcgctac
     1561 attataccaa gtgccattta tatacttttg aatcggtctg taataaacac ccgcaatatt
     1621 atcggcagag ttgaacctgt atcctgaaca ttaataccag acaatacatg acctgaaggg
     1681 cactgaaaat tccatgtttg ccagttgttc actccataat attgctgtga accaagtcga
     1741 acatctttca catatctgga atcaaaattg ccatagttgc cg
//
LOCUS       DADMEQ010000261         1773 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.263, whole genome shotgun sequence.
ACCESSION   DADMEQ010000261 DADMEQ010000000
VERSION     DADMEQ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.263"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..284
                     /locus_tag="JNZ05_004110"
     CDS             <1..284
                     /locus_tag="JNZ05_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567081.1"
                     /translation="KSDYLPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAG
                     IYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            286..890
                     /locus_tag="JNZ05_004111"
                     /pseudo
     CDS             286..890
                     /locus_tag="JNZ05_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
     gene            877..>1773
                     /locus_tag="JNZ05_004112"
     CDS             877..>1773
                     /locus_tag="JNZ05_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8567082.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLPDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRVF"
BASE COUNT          426 a          403 c          496 g          448 t
ORIGIN      
        1 aaaaaagtga ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg
       61 catttaatgg tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc
      121 gctactatgc cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg
      181 tgagccgtga cggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg
      241 ttctggatgt gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct
      301 gccactgtgc ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt
      361 aacgcagcgc tttccccgac agttttatca gtgattttta tggtcttatc tggaacatcg
      421 ataccgcaga aaagtatggt cttgatgtct ggggaaagat tgtgggtgtc agtcgccggc
      481 tgacggtaaa ggacgatttt aattacctgg gcttcagcga gtccaggatg gacaccccgg
      541 taatggatga tccctgtccg tttaatcagg caccgtttta caacggaaaa tcggataccc
      601 ggactgttga cctgtctgat gctgtatacc ggcggctgat actgatgaaa gccatgtcga
      661 acattactga ctgttccgtt ccggatatta acctgatgct gagatttatg ttcggaaaaa
      721 aacgccgggc ttatgttctg aataatggcg ggctgaggat gagttacgtc tttgagtccg
      781 cgctctcgtt ggcagaactg gcgattatcc agtcgtcggg tgcactgcca tccccgccgg
      841 gtgtttatgt ttcagtagtt ttaaaggagt cccgtaatga aggccagtga taaaccccgc
      901 cagctggcgg tcccctttgc gagtaccgga gataaaaacc gtatcccgga caaggcgaca
      961 cagcagacca gagagagcgg taatgctgcg tatgattcag gttttcctcc ggtgaccatg
     1021 acagcggtct cagcgggagg tataccgcca cacggcaagg attttaacgg tctgatgtac
     1081 gatattaccg cagcaatacg gttcgcccag gctggcggtt tgtacacgta taatgccggt
     1141 tttgcggggg ccattggtgg atatgcaaaa ggagccattc tcgccggagt cgcaacaaca
     1201 gcggtctggc tgaataccac ggacgataac ctgaccgatc ctgaaggctc cgacagtgcg
     1261 ggctgggtaa atcttcttga ggatccgaaa aggatattcc tgcggcagaa gaacaatctg
     1321 ccagaccttc agaataaagg gacggcacgg gataatcttc aggtttacag taaagagcag
     1381 tcagatcaac gctatgttca tcgggaaggc gataaaataa ccggagagct gaaaatccgt
     1441 ggtgttaatg cgctgaggat tttcaacgaa gcttttggcc tgatttttcg tcgttcggaa
     1501 gagtgcctgc accttattcc caccagtgaa ggtcaggggg aaaatggcga tattggtccc
     1561 ctgcgcccgt tcaccattaa tttgcggacg ggtgaaatat ccatgtcgca taaagtgtct
     1621 gttggcggtg gttcgcaggt caatggtgcg ctgggtatcg gcgttcagaa cgccctgggg
     1681 gggaattcaa ttgttcttgg tgataatgac accggattta aacagaatgg agacggtatt
     1741 ctggatgttt atgctaatag tcagcgtgta ttc
//
LOCUS       DADMEQ010000262         1746 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.264, whole genome shotgun sequence.
ACCESSION   DADMEQ010000262 DADMEQ010000000
VERSION     DADMEQ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.264"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            44..976
                     /locus_tag="JNZ05_004113"
     CDS             44..976
                     /locus_tag="JNZ05_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY8567083.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1052..1126
                     /locus_tag="JNZ05_004114"
     tRNA            1052..1126
                     /locus_tag="JNZ05_004114"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1085..1087,aa:Arg,seq:cct)
     gene            1288..>1746
                     /gene="intS"
                     /locus_tag="JNZ05_004115"
     CDS             1288..>1746
                     /gene="intS"
                     /locus_tag="JNZ05_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HAY8567084.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
BASE COUNT          492 a          398 c          438 g          418 t
ORIGIN      
        1 ccgctacgct ttagctatac gtgctaattc aagagaagag accatggaca acgacaaaat
       61 tgatcaacac agcgacgaaa ttgaagttga gagcgaagaa aaagagcgcg gcaaaaaaat
      121 agaaatagat gaagaccgac tcccctcccg agcgatggca attcatgagc atatccgcca
      181 ggatggtgaa aaagagctag aacgcgacgc aatggcgcta ctgtggtcag ccattgcggc
      241 gggtctgtcg atgggcgctt cgttactggc aaaagggata tttcatgtcg aactggaagg
      301 tgtgccgggc agcttcttgc tggagaatct cggttatacc tttggtttta ttatcgtcat
      361 aatggcccgc cagcaattat ttaccgaaaa caccgtgact gcggtactac ccgtcatgca
      421 aaaaccgaca atgagcaacg ttggcttact tatgcggtta tggggagtcg tgctgctggg
      481 taatattctc gggactggta ttgctgcgtg ggcatttgaa tatatgccta tcttcaatga
      541 agaaactcgc gatgcatttg tcaaaatcgg catggatgtg atgaagaaca cccccagcga
      601 gatgtttgcc aacgcgatca tttccggctg gctgatcgcc actatggtat ggatgtttcc
      661 tgcagcaggt gcggcaaaga ttgtggtgat tatattgatg acctggctta ttgccctggg
      721 tgacaccacc catatcgtgg tcggttctgt tgaaatcctc tatctggtgt ttaacggtac
      781 gctgcactgg agcgatttca tctggccctt cgcactacct actttagcgg ggaacatctg
      841 cggcggcacc tttatcttcg cgttaatgag tcatgcacag attcgtaacg acatgagcaa
      901 caagcgtaaa gcagaagcac gccaaaaagc agaacgtgcg gaaaacatta agaaaaatga
      961 taaaaaccca gcataaatgg cgagggttta agcaatcgag cggcagcgta cttaccccgc
     1021 agtccattag cgggtatact catgccgcat tgtcctctta gttaaatgga tataacgagc
     1081 ccctcctaag ggctaattgc aggttcgatt cctgcagggg acaccattta tcagttcgct
     1141 cccatccgta ccagtccgca aaatcccctg aatatcaagc cttccgtata ttcacagttc
     1201 gtcatggttc gcgtcagatc gttgacagcc gcactccatg acgggtaaaa agtggataaa
     1261 ataattttac ccaccggatt tttacccatg ctcaccgtta agcagattga agcagcaaag
     1321 ccgaaagaaa aaccataccg ccttctcgat ggtaatggcc tgtaccttta tgtccctgta
     1381 tccgggaaaa aggtatggca gcttcgctac aagattgacg gtaaggagaa aatcctgacc
     1441 gtcggaaaat atccgcttat gactttgcag gaggcaaggg ataaagcatg gactgcgagg
     1501 aaagacatct cggctggcat cgatccggta aaggcgaaaa aggcttcgtc taacaacaat
     1561 tcatttagtg cgatttacaa ggaatggtac gaacacaaga agcaagtctg gtcagtaggg
     1621 tatgcgactg aacttgcaaa aatgtttgat gacgacattt tacccatcat cggcggcctt
     1681 gagattcagg atattgagcc gatgcaactg ctggaagtaa tccgcagatt tgaagatcgc
     1741 ggtgca
//
LOCUS       DADMEQ010000263         1731 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.265, whole genome shotgun sequence.
ACCESSION   DADMEQ010000263 DADMEQ010000000
VERSION     DADMEQ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.265"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(219..773)
                     /locus_tag="JNZ05_004116"
     CDS             complement(219..773)
                     /locus_tag="JNZ05_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAY8567085.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(770..1060)
                     /locus_tag="JNZ05_004117"
     CDS             complement(770..1060)
                     /locus_tag="JNZ05_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY8567086.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1060..1659)
                     /locus_tag="JNZ05_004118"
     CDS             complement(1060..1659)
                     /locus_tag="JNZ05_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY8567087.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          416 a          477 c          393 g          445 t
ORIGIN      
        1 ggtggcgaac tgtgcagggt tggcgtaacc gggtacggaa accggcgagc ctttcggctc
       61 ccccacacag cccgccataa atcgcgaatg tgactgtgca aacgatatga aaaaagacgc
      121 gggcgcgtct catatcgctc cgtaaacatc cgggacgcca atcccgacgc cagattttgc
      181 tggcgcgtga ggaatatagc cccggataac agattgagtc aacaaacggt ttttagatcc
      241 ccggaagaga atgcatcacg catcggtaga tagagcatat attcagcaag atttagccat
      301 acatctaccc gactgcagca cgtccggaaa caccactcag ggtgtgcgtc attcagcaat
      361 tcagccattt tacgcttagt catcccccgc ccttcatacc gttgccggag gacactaatc
      421 aatccaggat gctctgccag cacctcactt atgactctat caatacataa cgcctctgca
      481 tcagtacaat gcgccagcca ggtcttttgc ttgccattga tcatctctcg caaaaacgct
      541 tccagctcag gtttctctat tcccgctttt ttcatcctgc gcagggcttc attgacggct
      601 gttttcgtca attttttgga tgccagcaac tggttaaaca tatttcccgt cttaccgccg
      661 ccaatatacg accagcgccc ccacatgcgc agttttccct gaatccagac actttccagc
      721 gtggtgagac gaaggtgttc cccgcttttg cctgtatttg ttgggtaaat cataaataac
      781 cttcctttct ccagatttct tgtgtgcgaa aaacaccttc tgcatgcatc aggcgtaatt
      841 cttctttggt gtaatcgctg gtttttaccc gcccgtcgat taaatcgtgg catgagctac
      901 aggcaatcgc tgcctgcata tcatgtggtt ttgtcgctgt tccgcacgtc cccgccagcc
      961 tgtaatgcgc cagcacagaa gtttcgggat tgtgattgca gtagccagga attctgacgg
     1021 tgcacatctg cccccgcgcc gctttacgta aatccaccat tacgcaaact ccagtaactg
     1081 cgcggccaca ttttcgactt cctccggaga ggaaaattta cggaacagga tccagttcca
     1141 cagcacattc agtacagatt tataaacctg ctgaaactcg gtttcgtcca tgttcgcaaa
     1201 tgcgatagat tttgccctgc gcccacgact accatcagga taaatatgct cggtgtaaaa
     1261 tccggcctga atggttaccc actcgcggaa agcgtcaaac gactttagca atgccgtatc
     1321 ccgggttctg catgtcgcaa ctgtattaag gtattgctct gcggcatcac tcagggctgg
     1381 agtgtgttcc cgacctactg attcgcacag gtaatcaacg aagcctgata ccagttttcg
     1441 ttcgcgaggc gtgatcgccc caccgaccgg agtccagtaa tcaaatccca gttgcaggag
     1501 tttgaaaaaa cgcttgtgga atgcgtagtt acgcacacgc ttaaagtctg cgtgtatcca
     1561 ctcacctatt ttgatttgat gcaaaaaatc gcaactctcc ggcgtcgccg ggagaagtaa
     1621 tccggaagag gtttgtttga ccagttgtat atgcgccatc gtagttctcc gctggcgcag
     1681 tagaatgggt gttcagcccg ttatgtagta taccagaatt aatgccaata c
//
LOCUS       DADMEQ010000264         1717 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.266, whole genome shotgun sequence.
ACCESSION   DADMEQ010000264 DADMEQ010000000
VERSION     DADMEQ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.266"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..219)
                     /locus_tag="JNZ05_004119"
     CDS             complement(<1..219)
                     /locus_tag="JNZ05_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY8567088.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNL"
     gene            525..1307
                     /locus_tag="JNZ05_004120"
     CDS             525..1307
                     /locus_tag="JNZ05_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAY8567089.1"
                     /translation="METKQKERIRRLMELLKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVNKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKAMNRGLRKILLARHALFDEVA
                     SASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(1309..1407)
                     /locus_tag="JNZ05_004121"
     CDS             complement(1309..1407)
                     /locus_tag="JNZ05_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HAY8567090.1"
                     /translation="MISQIDKLEYVVKVQRNQSNPTMFNKIVVFFQ"
BASE COUNT          481 a          408 c          343 g          485 t
ORIGIN      
        1 aagattacga atagtattat ccgcaaaaat atcatcacct actttagtca gcatcaggac
       61 ttttgaattc aatttagccg ccgccaccgc ttgattagca cctttcccac cacatccgat
      121 tttgaaggca ggtgcttcca gagtttctcc ttctttaggc atctgattag tgtaagtaat
      181 gagatccacc atattggaac caataactgc aatgtccatt tcactacctc ttataaactt
      241 tcgcataaca atggtattta aataacatta gtatgttact tttgcatcat ttgtgactga
      301 gatcgcgatt agcacatcaa cccgatgttt atttaataga cttccagtct catcactcag
      361 gccaacacta tctaatcata agcaacctaa caagattagt gcccaaaatt cagcagccta
      421 tacccttttc atttcaaagg ggcggtcgta tagtatggta atgaaaacaa tgtttactaa
      481 cgccaaaatg ttatttttat aacattctta cggagagaga gttgatggaa acgaagcaaa
      541 aagagcgtat ccgacgtttg atggaactgc ttaagaaaac cgacagaatc catttgaaag
      601 acgcagcgcg aatgctggaa gtttctgtaa tgactattcg tcgcgatctc catcaggaag
      661 atgaacctct gccactgacc ctactgggtg gctatattgt aatggtgaat aaacccgcgc
      721 catccatgcc agtaatccat gacgttccaa aaaatcatcg tgatgactta cctattgcaa
      781 ttctggctgc cggaatggtt aatgaaaatg atctgatctt ctttgataat ggccaggaga
      841 taccactcgt tataagcatg atcccggatg caatcacctt caccggcatc tgttactcac
      901 atcgcgtctt tgttgcgttg aatgaaaagc ctaatgtaac agcaatactt tgtggtggta
      961 cgtatcgtgc cagaagtgat gctttttacg atgccagtaa ctcttcgcca ttagactctc
     1021 tcaatccgcg aaaaatattt atttccgcca gcggtgtgca taatcacttt ggcgtcagct
     1081 ggtttaaccc tgaagatctt gccactaagc gtaaagcgat gaaccgtgga ctacggaaaa
     1141 ttttgctcgc ccgccacgcg ttgttcgatg aagtggcctc tgccagcctc gcaccgatct
     1201 ctgcatttga cgttctgatt agcgatcgtc cgttaccggc agattatgtt acgcactgcc
     1261 agaatggttc tgtaaagatc attacacctg attcagaaga cgaatgactt actgaaaaaa
     1321 caccacaatc ttgttaaaca tcgtcgggtt ggactgatta cgttgcactt tcaccacata
     1381 ttccagctta tctatttggc ttatcaccta ctccagacgc tggtcatcct tgaccagtag
     1441 ccagatatgg cttttgtcgc agtcctgaat cggcaggcag agaatgtctt caacgttaag
     1501 agcgcgacgg gcaaaagccc acaaaggtga gtcattacac ccggatggta aacaggcact
     1561 tccttgttgt agaagccgtt atattgtcca taatgtctgg gtttttaatg catactgggt
     1621 aacaaccgga cgaatcttcg aatatcactg gcgctgccag ccggtttgaa ttcatctcac
     1681 aaccctgcat agagcgaatc tcctgcctgc acgtcac
//
LOCUS       DADMEQ010000265         1679 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.267, whole genome shotgun sequence.
ACCESSION   DADMEQ010000265 DADMEQ010000000
VERSION     DADMEQ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.267"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(72..539)
                     /locus_tag="JNZ05_004122"
     CDS             complement(72..539)
                     /locus_tag="JNZ05_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAY8567091.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(605..844)
                     /locus_tag="JNZ05_004123"
     CDS             complement(605..844)
                     /locus_tag="JNZ05_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HAY8567092.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(979..1584)
                     /locus_tag="JNZ05_004124"
     CDS             complement(979..1584)
                     /locus_tag="JNZ05_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY8567093.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          542 a          370 c          294 g          473 t
ORIGIN      
        1 agacctactg ctatcacaat tactgagaga aaaaaaagca tccgatgatt catttatgct
       61 gtcctaaaaa attaacaaaa caatgggaaa acaggatatg taagcagaaa agccaatgaa
      121 attggtacag cataaggcac accgttttca cgaattgatt ttcgaaaaaa aagaagacct
      181 gccagaacaa ccagagcgcc gcaaaaggtc attgcgacga gaaaattagc cagttcatgt
      241 tcatcaatag ctaacgaaag aacagttaac agcttgacat caccgccgcc aattatccct
      301 gcggaaaaaa gaaagaagcc cacgagcaaa acgctcccgg ctgcaaccag attgggtttt
      361 tgatgctgta aaatgagtgc caaaaaaata agcacggtta acaccagtaa ataacgatgg
      421 ctaataattc tgagtaatat atctccccat gaaataaaaa taagaaagag cgcaacaaat
      481 aaaagatcga ctcttagtaa ccatattgct gccattgaaa gactccatgt accatacaag
      541 gttgctgcca gacatattcc tttacgtctg acagcctcta gagagttttt ttatttttta
      601 taagttacgg attactcgtt ccggaaatcg ttacctgttg aattgctgca gcgatagcgt
      661 cgaatgcatc tttaattgcg cccaggaagc ctgagttctg atcgccaaag atgaaagcaa
      721 gcagagttgc catagcaaca ccaatcaatg catattcgat tgcagtaaca ccacgttcat
      781 ccttcgcgaa acgcgcaaag cactgtttag ttttaccata gtacttagta ataaaagtat
      841 tcatgttttc ctcgtatatt attatttaaa ttcaaaccac cagcttcatc tgcgtgtcac
      901 cggtatccca tatttttggg atgtggctat aattatccat tcccgctatt tagtcttcaa
      961 aaaaaattac atttaatatc atttatcttg attaagaaat agtaagacta aaaaaacaag
     1021 cgtcgtcagc cagcacactg ctaacacggt aagaacgcta ataaaaacac tccactgctt
     1081 cgttctatac cattcaatat ttttgtcagt atgttttatt gggagcagct cttcagtaga
     1141 gaattcctgg ctgttatcat tctcaggaat gtttgcatca gtgtttactt ccctggcctc
     1201 gctatcgttg gtatagctac tactgtcttc atcactgtca cgctcattct cacagacatg
     1261 tttctcgacc tttcccagat atctgacgcc ttttcgcgga atggttttaa ttagagaact
     1321 cgacaaacct gcgacgtcta atgcctttct aagcatgtaa atctgcccat aataactgct
     1381 gtcgcttacc gaacctttct gctcccgcca aacctcattg ataatatgtt gcttatcgga
     1441 ttctccctgt agtaaaagga gtaaaaatcg atagccgttt tcagacagat tgattacatt
     1501 gcctgtactg gtggtgatac gtcgagacgc tgggtaaaac ttaactccct gccctaaaga
     1561 aaaataaaaa cggtcatcgc tcataagtac cgaaccctgc tttcttcttc acatgactac
     1621 tcattacaac tttgtatgaa gtaagatgca aaatcatatt gtttacatta agttaagca
//
LOCUS       DADMEQ010000266         1662 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.268, whole genome shotgun sequence.
ACCESSION   DADMEQ010000266 DADMEQ010000000
VERSION     DADMEQ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.268"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(1..933)
                     /locus_tag="JNZ05_004125"
                     /pseudo
     CDS             complement(1..933)
                     /locus_tag="JNZ05_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
     gene            complement(961..>1662)
                     /locus_tag="JNZ05_004126"
     CDS             complement(961..>1662)
                     /locus_tag="JNZ05_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY8567094.1"
                     /translation="ESWGINTTYVEKVPCTSSGVAPIFVNANSSNSILIIKGANKFLS
                     PEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVLLNPAPALRELDMSYA
                     CKGDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNIIVTMSEKGALWMTRDQ
                     EVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAALFAAFSVTGKGMQSS
                     YPSIEQFNEFLTLNE"
BASE COUNT          519 a          366 c          328 g          449 t
ORIGIN      
        1 tcataaggat gttggctata aacttgccaa taaagaagca ggcaaagctg tagaccatga
       61 agtttgaagc atcacgttcg ttgatatcgc ccaactccag tgccagacgg atggtaaatg
      121 accatactgc gacctgcata cccacataaa ggaattgagc cacaatacca cgacgaaagc
      181 gcgaattttt agccagatag cgcagcgtat ccattgctga cgggcgttta tggtgacttg
      241 tctgtgccac tttacaggtt ggaaagcggg ttaaaaggaa caacaccatg accacaacca
      301 gaatcataat catatactta tacggttcaa gggtgttctc taacatcagc accttaaagt
      361 tgtgaatttg ctcggcgttc attccggaca tctgcttctc aaggctttcc ccctcggaga
      421 aaaccagata tttgcccaat aaaatacctg acgcagcacc aatcggataa aaggtctggc
      481 tgatattgag ccgcaatgtg gcataggctt ttggaccgat cattgaactg tatgtgttcg
      541 ctgcagtttc aaggaaactc aggccaatcg caatcgcaaa aatagctgca aggaacatag
      601 tgtaggttgc catatgcgag gcagggaaaa aagtgtacaa ccaccaatat acagcgtcag
      661 gccaattaaa attgccacct tataactggt ctttttaatc acaagggatg ctggtattgc
      721 aattaaaaaa taacctccat aaaatgcgct ctgcaccaat gctgaagcaa agttacttag
      781 cgaaaataca cttttgaatt gagtgattaa tatgtcattt aatgcagctg cgcatcccca
      841 tagcgggaat aaacacgata acaaaataaa ctggaacaag ggagtcttat tcagataccc
      901 atcaggcatc tgaatgatgt ttttatcgtt catagtgcta cctttaactg tgcaggatga
      961 ttattcgttt aaggttaaaa attcattaaa ttgttcaata ctcggataag atgattgcat
     1021 acctttccct gtgacgctga aagcggcaaa gagagcggct tttttcaaag cggcttcaac
     1081 atcaccgctt tgaacataat aatgggaaaa gcaaccaata aatgcgtcac cagcgccact
     1141 agtatcaaca gcatttactt tgaatgcagg aacatggact tcctgatcgc gggtcatcca
     1201 taatgcgcct ttttcgctca tggtaacaat aatattgttc agccctttat caactaacga
     1261 acgtgcggcc aaacgaatat gatcataagt atcaaccgac ataccggtta atatttccag
     1321 ttctgtttca ttcgggataa agaaatcacc tttgcaggca taagacatat ctaactcacg
     1381 caatgccgga gccggattta ataacacttc aataccattt ttcttaccaa actcaatcgc
     1441 gtggtaaact gtttccagtt gaacttccag ttgtaaaacg atcaatttgc attttttcag
     1501 atcttctgca gctcgatcga tatcttccgg ggaaagaaat ttattcgctc ccttaattat
     1561 taatatacta ttgctcgagt tggcattaac aaagatcggt gcaacaccac tgctggtaca
     1621 ggggaccttc tcaacataag tggtattaat tccccatgat tc
//
LOCUS       DADMEQ010000267         1655 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.269, whole genome shotgun sequence.
ACCESSION   DADMEQ010000267 DADMEQ010000000
VERSION     DADMEQ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.269"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(156..1391)
                     /locus_tag="JNZ05_004127"
     CDS             complement(156..1391)
                     /locus_tag="JNZ05_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAY8567095.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="JNZ05_004128"
     tRNA            complement(1538..1614)
                     /locus_tag="JNZ05_004128"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
BASE COUNT          420 a          452 c          394 g          389 t
ORIGIN      
        1 gccaccgcac gcaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gccatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtc
//
LOCUS       DADMEQ010000268         1605 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.270, whole genome shotgun sequence.
ACCESSION   DADMEQ010000268 DADMEQ010000000
VERSION     DADMEQ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.270"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..>1605)
                     /locus_tag="JNZ05_004129"
     CDS             complement(<1..>1605)
                     /locus_tag="JNZ05_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY8567096.1"
                     /translation="IYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQRFTQPYA
                     SLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQAAENYGSA
                     ALGVGKELGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSLRLVGYRY
                     STEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLSRQQYWHT
                     DDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLSLSVPLSG
                     WWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVGGNMSLTY
                     DGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAPGAAGLEI
                     DNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPTRGAVVKA
                     EFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSGLPEKGQV
                     TLSWGENASTKCIFNYSLSTPESESG"
BASE COUNT          412 a          417 c          346 g          430 t
ORIGIN      
        1 atccgctctc actttctggt gtcgaaagtg aataattgaa gatgcatttt gttgaggcgt
       61 tttctcccca ggataacgta acctgtcctt tttctggtag accagaaaga taaagttcgc
      121 cacggtcacc gacaattcct gaatctgcca aacttgcatc ttgtatcgca gcaatggctc
      181 caaaaggtac cggtttacca tttgcattta acactctgaa gagaacgcga taacccacat
      241 gagtcacaaa ctccgcttta accactgcac cacgcgtggg aaccatcgta acgacggtat
      301 tatcaagttc tatattcgac ttcgagaaat agttatcgct gattgctacc cgattttcat
      361 cataagggtt tagctgtgtc ttgaccgtat agccgcgcca gtccgtcgca gcaccgcgca
      421 tattatctat ttctaacccg gcggccccag gcgctttaac aagagcaata gtttcaccta
      481 actcctgact taacgttacc ccttcgctgt gtgccagaat tccccctctg ataccataat
      541 tcagcctctg ggaattttgg ctgtagttat agctgccgtt caccgtgcca taagagccat
      601 cataggtcag ggacatatta ccgccaacct catcatcatg actgttatag gactgcatta
      661 aattccatga aaggttattt ctttccagcg ccgtaccgct gagtccgagt tgatgtgagc
      721 taccggaatt atcgttttgc gaaacagaat aggtggcgta actattaccc caccagcccg
      781 ataaaggaac agataaagag agcatgtaga tctgctcact ggtgttatca ctggcatggt
      841 tgttccccgt cccctgtctg gcagtattgg aataactcca ggagacgttc cacgagagac
      901 gcttccagct actgctgtag ccaaattgca ttaatcgttc gacatcatcg gtatgccagt
      961 attgttgccg gcttaatgtc aggtataaat tgccataatc tctccccaac gactgcgtta
     1021 gcgttccctc cacgcgacta cgtcggttac ctgtttccca aaagtcttca gggctgttgc
     1081 gccgcgatgc ccactcattg agggtatagt agccctcggt ggagtaacga tagccaacca
     1141 ggcgcaagct agtgtctgtg tcgtcaaata gttttgaata gagaaagcga tatgactgac
     1201 ccgtttctgt atcatcatgg ctaaaattcg cacgagcatg tgtcacatcg aaagaaattg
     1261 cgcccaaagc gccaagctct ttaccgacac ccagagctgc agaaccataa ttttcagcag
     1321 cctgcatacc tccatataaa gtgatcccgt gggaaaaacc atgaagtatt tgcgcctgaa
     1381 ggacgtccgg tgtaaatccg tcttcatcgc gcaattcacc cacgctgaca tcaacatctg
     1441 tcagaccttc acgtttaaga atcgccaatg aagcataagg ttgggtgaat cgttgttcgc
     1501 taccgtcttc ttctttgatg gttacgtcca aatcgccacc tgtacttgcg gtgttgagat
     1561 cggtgatctc aaaagcacct tgtggaacgg ctgactgata aatca
//
LOCUS       DADMEQ010000269         1604 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.271, whole genome shotgun sequence.
ACCESSION   DADMEQ010000269 DADMEQ010000000
VERSION     DADMEQ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.271"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..>1604
                     /locus_tag="JNZ05_004130"
     rRNA            <1..>1604
                     /locus_tag="JNZ05_004130"
                     /product="23S ribosomal RNA"
BASE COUNT          445 a          349 c          499 g          311 t
ORIGIN      
        1 gacgtgctaa tctgcgataa gcgtcggtaa ggtgatatga accgttataa ccggcgattt
       61 ccgaatgggg aaacccagtg tgattcgtca cactatcatt aactgaatcc ataggttaat
      121 gaggcgaacc gggggaactg aaacatctaa gtaccccgag gaaaagaaat caaccgagat
      181 tcccccagta gcggcgagcg aacggggagc agcccagagc ctgaatcagt gtgtgtgtta
      241 gtggaagcgt ctggaaaggc gtgcgataca gggtgacagc cccgtacaca aaaatgcaca
      301 tactgtgagc tcgatgagta gggcgggaca cgtggtatcc tgtctgaata tggggggacc
      361 atcctccaag gctaaatact cctgactgac cgatagtgaa ccagtaccgt gagggaaagg
      421 cgaaaagaac cccggcgagg ggagtgaaaa agaacctgaa accgtgtacg tacaagcagt
      481 gggagcacgc ttaggcgtgt gactgcgtac cttttgtata atgggtcagc gacttatatt
      541 ctgtagcaag gttaaccgaa taggggagcc gaagggaaac cgagtcttaa ctgggcgtta
      601 agttgcaggg tatagacccg aaacccggtg atctagccat gggcaggttg aaggttgggt
      661 aacactaact ggaggaccga accgactaat gttgaaaaat tagcggatga cttgtggctg
      721 ggggtgaaag gccaatcaaa ccgggagata gctggttctc cccgaaagct atttaggtag
      781 cgcctcgtga attcatctcc gggggtagag cactgtttcg gcaagggggt catcccgact
      841 taccaacccg atgcaaactg cgaataccgg agaatgttat cacgggagac acacggcggg
      901 tgctaacgtc cgtcgtgaag agggaaacaa cccagaccgc cagctaaggt cccaaagtca
      961 tggttaagtg ggaaacgatg tgggaaggcc cagacagcca ggatgttggc ttagaagcag
     1021 ccatcattta aagaaagcgt aatagctcac tggtcgagtc ggcctgcgcg gaagatgtaa
     1081 cggggctaaa ccatgcaccg aagctgcggc agcgacgctt atgcgttgtt gggtagggga
     1141 gcgttctgta agcctgtgaa ggtgtgctgt gaggtatgct ggaggtatca gaagtgcgaa
     1201 tgctgacata agtaacgata aagcgggtga aaagcccgct cgccggaaga ccaagggttc
     1261 ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa ggcgaggccg aaaggcgtag
     1321 tcgatgggaa acaggttaat attcctgtac ttggtgttac tgcgaagggg ggacggagaa
     1381 ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt gtaggctggt tttccaggca
     1441 aatccggaaa atcaaggccg aggcgtgatg acgaggcact acggtgctga agcaacaaat
     1501 gccctgcttc caggaaaagc ctctaagcat caggtaacat caaatcgtac cccaaaccga
     1561 cacaggtggt caggtagaga ataccaaggc gcttgagaga actc
//
LOCUS       DADMEQ010000270         1602 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.272, whole genome shotgun sequence.
ACCESSION   DADMEQ010000270 DADMEQ010000000
VERSION     DADMEQ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.272"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(1..275)
                     /locus_tag="JNZ05_004131"
                     /pseudo
     CDS             complement(1..275)
                     /locus_tag="JNZ05_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(390..1028)
                     /locus_tag="JNZ05_004132"
     CDS             complement(390..1028)
                     /locus_tag="JNZ05_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567097.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1187..1602)
                     /locus_tag="JNZ05_004133"
                     /pseudo
     CDS             complement(1187..1602)
                     /locus_tag="JNZ05_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          448 a          404 c          288 g          462 t
ORIGIN      
        1 gccagtctac ccagcaagcg ctgtaactct tttatttgtt ttatagcggc agccagttct
       61 gaggcaggaa cgacatcctc tccggcagca acagcagtga gacttccttc cagatattat
      121 tttttccact taagcaacag gctgggctga ataccatgca aacgcgccac atgggacacc
      181 gtcatgccag gctccattgt ctgctgaata atggcaattt tttcctgagg agttttacgt
      241 ttacgaagct ccagccccag caaagttcca gtcatctcaa aattagaact agtgttagac
      301 atatatccaa gcctatctct tattttaaga tacatccact gtctggcgtt tcaaggggcc
      361 ggctcaaatc tatgtacagg ctctcagcat cagcaaagcc tcttactttt ccaactacta
      421 ctgttagcat agtgatcaaa ttcctgatca gatacatgaa ttacctgaac cgaactattt
      481 tcatctacaa atgcatctgg cgatttttct acaggatcat aaagattaaa tccctttcct
      541 gtttgaacat ccaaaaacat aattttttca ccatcgttaa atccagctat ccaatgagtg
      601 gcctgttcaa tagaaatcat tactacactt ccttttgaag tattttgtaa aatattttct
      661 gcaatacgct cacgaaattt actataagtt tcattaggat gtgacactat gccttcacta
      721 tcaatttgct ctaaatgcct atgaaaaaat ccggtcatat actctgatat ttcattaggt
      781 acgggagcct ctcttactcc ataccctaac atagccatac aagcatgaag cgcacaccct
      841 gaacagttac catccccacc agacggattt atcattttct gtaaagtcgc tgccaattca
      901 gaatctttat ccatatcacc aagactttta gagtttaaag attccagcaa ttttttaaca
      961 tttacatttc cctcctctgt atgtgcctca tatccagcag ttccctgtaa cgacacccca
     1021 ttaatcatat tgtatcctga cagtatatat actcactata ttttcttcat aacaaatatc
     1081 cctctgatat gacaacaaaa aacggagccg gattcctgtt tttgtgaact gcatctattc
     1141 gtgtctaaaa agtgatccac ttaccaccaa cggcctaata tatacagaac ggagtaaaca
     1201 gggctaacag ttcccgacag gtctcatcat tccgtggccc gaaagtgtaa gccaacacac
     1261 cgccagtttt agtgttgtac gcataccaga gccagtgttg ttgcagcctt gctgccaacg
     1321 aagcgccact gctcatcaag ttcacagata agggcaacat cagcgtgagc gactggcgca
     1381 gatgttatcc gccggggcgg gagcttttta atgtccgtat gacggtattg atgccgattt
     1441 tcagtgtcct ggcggtatca cggccccctg cgccgttatg tgccatttca acgatctgct
     1501 ccttaacgcc gggttttctg gcttcataag agtaagtgag ctggaaaaca cgtttacatg
     1561 agcgacaacg gaaacgctca tgccgggaac aactacggcc at
//
LOCUS       DADMEQ010000271         1552 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.273, whole genome shotgun sequence.
ACCESSION   DADMEQ010000271 DADMEQ010000000
VERSION     DADMEQ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.273"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..1144
                     /locus_tag="JNZ05_004134"
     CDS             <1..1144
                     /locus_tag="JNZ05_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567098.1"
                     /translation="NDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQTSIQMVTL
                     LNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSDNTCELTIN
                     EPLKNQEISTGNINVNCPLKDIYNEIRRLNVIFSCGTGDIVDLSSLDLRNVDLDYYDF
                     TDKHMANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKITPVLISISDMY
                     SEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELTKEIYKSSSKI
                     NNCLNRIKLPEMKEFSSKKIYDYIDTIIEDYENIKENAYLVVPQINYTMDLNIEDSSS
                     EELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT          561 a          286 c          260 g          445 t
ORIGIN      
        1 taatgatatt agtagtgatt tagtccgtcg aaaaatgaat gaccttcctg aaagccccat
       61 aggaaataac ctcgaagcac tagcacccgg tatagaaaaa ctaaaacaga cctctattca
      121 aatggtcact ttacttaacg cgttacaacc aagtggaaaa tgcattatca ctggtgattt
      181 tcaaaaagaa ttagcgtact tacaaaatgt aattctttat aatgactcgt ctcttcgtct
      241 ggatttttta ggttataacg cccaaattat tcaacgatcg gacaatacct gcgaacttac
      301 cattaatgaa ccgttaaaaa accaggaaat atctacaggc aatatcaatg ttaattgccc
      361 attaaaagat atttacaatg aaatcaggag gttgaacgta atctttagtt gtgggactgg
      421 agatatagtt gatctatcct ctctggactt acgtaatgtc gatttagatt attatgattt
      481 cacagataaa catatggcta atactattct aaatcatttt aaattggatt caacaaattt
      541 tactaatgcc aacatgtttc aggttaattt tgttagttca acacaaaacg ccacaatctc
      601 ctgggattat ttactaaaaa taacgcctgt tttaataagc attagcgata tgtattctga
      661 agaaaaaatc aagtttgtcg aaagttgttt aaatgagctt ggagacatta ccgaagaaca
      721 attaaaaatt atgagatttg caattataaa atctatacca agggcaactc ttacagataa
      781 attagaaaat gaattaacaa aagaaatata taaaagctca tcgaaaatca acaattgctt
      841 gaacagaatt aaattaccag agatgaaaga attctcatca aaaaaaatat atgattacat
      901 cgatacaatc attgaagatt atgaaaatat caaagaaaat gcttatctgg tcgtccccca
      961 aattaattat actatggatt taaacataga agactctagc tcagaagagt taataccctc
     1021 gagaaagacg aaaattctcc ggacaatggc tttgaggtcg gggaatataa cacatatgaa
     1081 gcatataact cagagaatca atattttacc agagaggact atacgtataa ttacgacctt
     1141 ttaaatgcaa tatagactta ataacttctt acaaagtcta ttattaatac cgtagatatt
     1201 catcttaccc tgtgcaccaa aagcaaaaac ttatcgacgc gggtttgaaa ctgatggcaa
     1261 ccgcaaaaat gcctgatgcg ctacgcttat caggcctacg ccatctctgc aatatattga
     1321 atttgcgtgc ttttgtaggc aggataaggc gttcacgcag catccggcat gaataaagcg
     1381 cactcaacaa tctgaaaccc gccggaacgg tttattacgt acatcaggtg aaactgaccg
     1441 ataagccgct ttcttttggg tatagtgtcg tggacagtca ttcatctttc tgcccctcca
     1501 aaagcaaaaa cccgccgaag cgggttttta cgtatatcag gtgaaactga cc
//
LOCUS       DADMEQ010000272         1517 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.274, whole genome shotgun sequence.
ACCESSION   DADMEQ010000272 DADMEQ010000000
VERSION     DADMEQ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.274"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(248..322)
                     /locus_tag="JNZ05_004135"
     tRNA            complement(248..322)
                     /locus_tag="JNZ05_004135"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Gly,seq:tcc)
     gene            complement(326..401)
                     /locus_tag="JNZ05_004136"
     tRNA            complement(326..401)
                     /locus_tag="JNZ05_004136"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(366..368),aa:Thr,seq:tgt)
     gene            complement(407..480)
                     /locus_tag="JNZ05_004137"
     tRNA            complement(407..480)
                     /locus_tag="JNZ05_004137"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(444..446),aa:Arg,seq:tct)
     gene            complement(482..557)
                     /locus_tag="JNZ05_004138"
     tRNA            complement(482..557)
                     /locus_tag="JNZ05_004138"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(522..524),aa:Ile,seq:cat)
     gene            complement(734..1423)
                     /locus_tag="JNZ05_004139"
     CDS             complement(734..1423)
                     /locus_tag="JNZ05_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HAY8567099.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQGNAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRLEQKA
                     AFSKQSIAKVD"
BASE COUNT          394 a          408 c          321 g          394 t
ORIGIN      
        1 cccggggttt gcttagaata ttttgtaagt tgtccgaaga gatatttaca acaccagaat
       61 gatgaatcat cagtcccctg ccagaggttc actacgtaac ctgaccgaca atgcaccatt
      121 ctggtgctga aaaaagagca ctggaactgc aacaaaacaa catcacagaa caggagatgg
      181 taaggagtca gacattccca cacaatattg tgtcagtgca aataacaacc ttcgtctcaa
      241 atctcgctgg agcgggcagc gggaatcgaa cccgcatcat cagcctggaa ggctgaggta
      301 atagccatta tacgatgccc gcatatggtg ccgactaccg gaatcgaact ggtgacctac
      361 tgattacaag tcagttgctc tgcctgctga gctaagtcgg cgcaggccca ccaccgagga
      421 cttgaacctc ggaccgtcaa cttagaaggt tgatgctcta tccggatgag ctagtggtgg
      481 ttggtggccc ttgctggact tgaaccagcg acctggcgat tatgagtcgc tcgctctcac
      541 cactgagcta aagggccgga ggcagaataa taaccatatg tcatcacatc tgcaaactca
      601 tctgaccacc agcacgttta acgtcctgtg ccatttttca ggcataaaaa aaccgctcgg
      661 cggcgggttt tgcttacttt accatcgcgt acaaaatcgg caaagtatca gatttgcatg
      721 aaatatatgc cttttaatct acttttgcaa tactttgctt tgaaaatgcc gctttttgtt
      781 ctaaacgtgt tctcatcaca aacaataaag cctcactatc cagtcggtga aaaatgtgtt
      841 tcattacaac ccagtgacca gtaaatgttt tggaccagtt tttggttgtt actcccgcca
      901 gtaatgccag ctcctgatat tcataacctt ccccaccaaa aagttctgct tttaccgcct
      961 gcgccgccag ccagattaat tttttcaggc gttcctgtgt tttctctgca attttcctag
     1021 tgccagaatg agccttaaac tcttcccacg cccagtgggt tatggtgatc tgatactccc
     1081 agcaaatact tccactgtaa caccacaaca accaggcttt atggtgctct tcaagagaca
     1141 gaacagcccg ccgccacgat gatgtcgaaa acgcaacctg actgaccaga ggaattgacg
     1201 cccccttcgc cagtgattgt tttcccggga ttggtggatt atcccgcgtt atcatttttc
     1261 cggtcacctc atcgcggtac cggatttttt tacgcctgta acgccctgta tcgaacatgg
     1321 cattcccctg ccaggcttca agctgacctt ttgtcgcccc actcaaatca gcggtggcga
     1381 taatgagctg ctcacgaaca aactgtaaat actggttatt catgcacact ccagctctgt
     1441 gatttttatc cccaaccgac caccaggaac aggcagtccg cgcacaatat tggtttcatc
     1501 aaactgctcg tcgtcga
//
LOCUS       DADMEQ010000273         1487 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.275, whole genome shotgun sequence.
ACCESSION   DADMEQ010000273 DADMEQ010000000
VERSION     DADMEQ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.275"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..1046)
                     /locus_tag="JNZ05_004140"
     CDS             complement(<1..1046)
                     /locus_tag="JNZ05_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HAY8567100.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          446 a          239 c          314 g          488 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtgt ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctattttat aatgactgtg tattcccatg gaaggagatc acccaat
//
LOCUS       DADMEQ010000274         1474 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.276, whole genome shotgun sequence.
ACCESSION   DADMEQ010000274 DADMEQ010000000
VERSION     DADMEQ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.276"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            122..469
                     /locus_tag="JNZ05_004141"
                     /pseudo
     CDS             122..469
                     /locus_tag="JNZ05_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707861.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            486..1004
                     /locus_tag="JNZ05_004142"
                     /pseudo
     CDS             486..1004
                     /locus_tag="JNZ05_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          450 a          336 c          332 g          356 t
ORIGIN      
        1 ctttaaatga agcacgactt gagcgagata tattaaaaaa agcaacagcg tattttgcac
       61 aggagtcgct gaaaaatacg cgttagtcga acaatggcga caacaattcc ctgttgaagc
      121 gatgtgtcgg gtacctggtg tatccaggag cggttattac gaccgggtac agcatgcccc
      181 ctcagacaga aaacaaagtg atgagcggct aaaactggag attaaggtgg cacatatccg
      241 cactcgcgaa acatatggaa cccggcggct ccagacggag ctggcagata acggaattat
      301 cgtcggtcgt gacagactgg cacgtcttcg taaggagctg aggctgcact gtaagcagaa
      361 acgcaagttc agggcgacca caaactctga ccacaatctg ccggttacgc cgaatctgct
      421 gaatcagaac tttacgccca cagcacctaa tcaggtctgg gtcgcagatt cagttgtcca
      481 ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt atgcagttcg
      541 gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggcg gatacaggaa gcacaggagc
      601 gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat gacccggtac
      661 tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca cttccccgtg
      721 ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt gaactcagtc
      781 cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt aaggttatcg
      841 aaaaacaacg ccctaaactg gcctgtcgcc ggtgcgacca tatcgtgcag gcaccggtgc
      901 cttcaaaacc cattgcacgc agttatgccg gagcggggct tctggcccat gttgttaccg
      961 gaaagtatgc agaccatctg ccattatacc gccaaagtga tctactattc cacacagcta
     1021 tttagctctc tttttgttga gctcccatga ttctttcatt tattgttact aattatttta
     1081 tgttatcatg atgttagaac acgctctgct ctgtgtctct attttatcta tcatattctg
     1141 aggattaact acataacaag gcgcaagata tatatccaaa aacaaataat accgttgcaa
     1201 taattccttt cagtaaagga ggtgtgatta atttcagggg atttcagagt aaaaattgta
     1261 caacatatta aaatatgttc atttcgttat aatacacaca agaaaatatt aagttagata
     1321 atatattcta aataaccata gataaaaacg cacataatgg ggaatatttt atgaaaatac
     1381 ctgaagcagt aaatcatatt aatgttcaga ataatattga tcttgttgat ggaaaaacga
     1441 atccgaataa agcaacaaaa gcattacaaa aaat
//
LOCUS       DADMEQ010000275         1440 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.277, whole genome shotgun sequence.
ACCESSION   DADMEQ010000275 DADMEQ010000000
VERSION     DADMEQ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.277"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            278..547
                     /locus_tag="JNZ05_004143"
     CDS             278..547
                     /locus_tag="JNZ05_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567101.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            799..1389
                     /gene="ospG"
                     /locus_tag="JNZ05_004144"
     CDS             799..1389
                     /gene="ospG"
                     /locus_tag="JNZ05_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HAY8567102.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          413 a          287 c          351 g          389 t
ORIGIN      
        1 ccgtcagctg actgacgagg tactggccct gcgattgtct gaaaacggct cacaactgca
       61 ccattcataa tcacatcgca taaaactgtc agcgccagtg ataggacagg tgaaacctgt
      121 caatacgtac cggcttacgc cgggcttcgg cggttttact cctctgccat atgcaacaac
      181 ggagtgccgc cttacatgcc gctggcgcgg catatcctgg tgacgatatc tgaacagtta
      241 tatactgtgt atatacgtgg taatgacgag gtggcaaatg gcacaggtta atatgagtgt
      301 aagaatcgac gcagaactga aggatgcttt tatggccgct gcaaaaagca tggaccgtaa
      361 tggctctcag ttaatccggg attttatgcg ccagaccgtt gaacggcagc acaattcttg
      421 gttccgtgac caggttgcgg caggccgtca gcaactcgaa tgcggtgatg tgcttcccca
      481 tgacatggtc gaatcctctg ctgctgcatg gcgggatgaa atgagcagga agattgctga
      541 taaatgatgg agatgttctg gaccatgctg gccatccagg acaggaagcg cattcgtagt
      601 acattgcgga gcagaacctg atggccgcca ttgaaccgga cgaacgggtt ggttattcag
      661 ccagcagcct ggcagggcag ccgtataaag gccgcaacgg tcgtgtggaa ggaacatctg
      721 gaccacacaa ggtagcgtgt aacgttattt tgtgtgaaaa tctcttgtga tttttaaata
      781 tcgtaacaaa ggtatgacat gaaaataaca tctaccatta ttcaaacacc ttttccattt
      841 gagaataata attctcatgc tggcatagta acggagccca ttctcggtaa gttaataggt
      901 caggggtcga cagcagaaat ctttgaagat gtgaatgatt catctgcttt gtataaaaag
      961 tatgatctta ttggcaacca gtacaatgag attctggaaa tggcttggca agaatctgag
     1021 ctttttaatg ctttttatgg cgatgaagca tccgttgtta tacagtatgg cggagatgtg
     1081 tacctccgaa tgctgcgcgt gcctgggact ccccttagtg acattgatac agctgatatc
     1141 cctgataata tagagagcct ttatctacag ttgatatgta aattgaatga gttgagtata
     1201 atccattacg atcttaatac aggtaatatg ctgtatgata aagaaagtga aagtttattc
     1261 ccaatagatt ttcgcaatat ttatgctgaa tattacgctg caaccaaaaa agataaagag
     1321 attatcgacc gacgattaca aatgcgtaca aatgattttt attcgttatt aaacaggaaa
     1381 tatttatagc cgtatttgtt gatgctataa gccagaggct cttctatgag aatatgggga
//
LOCUS       DADMEQ010000276         1433 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.278, whole genome shotgun sequence.
ACCESSION   DADMEQ010000276 DADMEQ010000000
VERSION     DADMEQ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.278"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..395
                     /locus_tag="JNZ05_004145"
     CDS             <1..395
                     /locus_tag="JNZ05_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HAY8567103.1"
                     /translation="EKDGFTQPVVVSEEKNHYLVVDGFHRQLLGREADTGKRLRGWLP
                     VACINPERKGQAARIAATIRHNRARGKHQITLMSDIVRDLSRLGWTNERIGTELGMDQ
                     DEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            623..1023
                     /locus_tag="JNZ05_004146"
                     /pseudo
     CDS             623..1023
                     /locus_tag="JNZ05_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1034..1405
                     /locus_tag="JNZ05_004147"
                     /pseudo
     CDS             1034..1405
                     /locus_tag="JNZ05_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          435 a          235 c          331 g          432 t
ORIGIN      
        1 tggagaaaga tggtttcaca caaccggtgg tggtgtcaga ggagaagaat cattatctgg
       61 tggtggatgg ttttcatcgt cagttactgg ggcgggaagc agatacagga aaacgcctga
      121 ggggctggtt gcctgtagcc tgtattaatc cggagagaaa gggacaggcc gcacgtattg
      181 ccgcgaccat tcgacataac cgggccaggg ggaaacacca gatcacttta atgtctgata
      241 ttgtcaggga tctttcacgt ctgggctgga cgaatgaacg tattggtaca gagctgggaa
      301 tggaccagga tgaagtgctg aggctgaaac agattagcgg gctgacagag ttgtttcagg
      361 aagaggattt cagctcggct tggacggttc gttgatgttg ttaaatatgg gcggtatttc
      421 tgaaagttga tactttgggt agaaaaaatg ataccgaaaa ctatggagga tgtgcttgtc
      481 aggtgactgg acaccatagg tcttaggctt tgataacggg ataaaatgag cggcttttct
      541 cataccatca ggtgtgatga tggcgcagat ttacaaaatc tgattgattc taaagatgta
      601 taagtaaaaa tcaagctttg atatgaaaaa acagatattt attaataata aacctcctgt
      661 agtcccttac tcagggacgc acgcgaaaat atttaaatac attgaaatcc ctctgccgtt
      721 tttctacttt atctatacat caggcgaacc attccacata tctgtccaaa atactgttat
      781 ttatgtatca aaatacaatg gtatatttat taataaatta gttccttttt ctcttttatt
      841 tgacagggat atatctgttc ttcaaagaag agatatttgt gttgtgcgat ttacgtcaga
      901 ggaaataagt gaacataacg ttttatttga tcatgacatt gaacgattaa aaaaatttcc
      961 aaagctcagc ttatatctcc cgattatgtt ttgattgatt tttctagtgg gggggggaga
     1021 tgaaccctat gcaatgtcct ggttaatatc tcagtgtgct catcagtgca ctgataataa
     1081 aaagacggag acagatgcca tttacgataa agttcggagt tcttatttgc tttcatgtat
     1141 tctgaaaaaa aacaaaaatg ttgggcttat tcttcatgca ccatcatttg tttctgtgtc
     1201 tgaaaaaatc gccagaattg tgatggcaaa ttactcccgt aactggagta attccgagtt
     1261 ggccagtgcg gttttaatga gtgaatcttc tttaaaaaga agaatgtaca aagaggtcgg
     1321 ttctataagt acatttgtac ataaaataaa gcttacagaa gctatcagga aattacgaag
     1381 gacaaatact cccatttcgg taatgctgcc aacttactga tttagtgtat gat
//
LOCUS       DADMEQ010000277         1401 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.279, whole genome shotgun sequence.
ACCESSION   DADMEQ010000277 DADMEQ010000000
VERSION     DADMEQ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.279"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..177)
                     /locus_tag="JNZ05_004148"
     CDS             complement(<1..177)
                     /locus_tag="JNZ05_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY8567104.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRT"
     gene            235..630
                     /locus_tag="JNZ05_004149"
                     /pseudo
     CDS             235..630
                     /locus_tag="JNZ05_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(631..1275)
                     /locus_tag="JNZ05_004150"
     CDS             complement(631..1275)
                     /locus_tag="JNZ05_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY8567105.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAATPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          349 a          322 c          337 g          393 t
ORIGIN      
        1 cgtgcgggaa ccaggagtac cgagcccttt tctggcggcg gtaacccatt gtcctaaagt
       61 gccttcagga agggataatc gggaagcgcc ttcactgatc gaaagttgat tttcaagaac
      121 cgttctgaca gcttcggctt tgaactcttt agagtaacgt tgggtttttc tgctcattat
      181 tagctccttc tgatgccatt ctatttcagg aaggagtgtc cgttaaactc aggctacctc
      241 accctggtgc tggtggcaat tctgattatg ctgccgactc tcggcatcgg tgatatgaac
      301 aatctctata aatggctgtt gaatcttaac tcggtagtga tgccgctgcg ttatttatgg
      361 gtatttgttg catttattgc agtcgttcgc ctggcgcaga aatataaacc agaatatgtc
      421 tttattcgta ataagcctct ggcaatgacc gtcgggattt ggtgttttgc ctttaccgcc
      481 tgtgcctgtt tgacggggat cttcccgaaa atggaagcct tcactgcaga gtggaccttc
      541 cagttggcgc tgaatgttgc aacgccgttt gtgctggtag gattaggact gatattcccg
      601 ctgctggcgc gtaaagcgaa tagcaaataa ttattgtggt cggtgacgct gactctggca
      661 gatctgaccc gtttttacat aatcgagaaa tgcgcgcaag ccgcaggaca tatactggcg
      721 gtttgggtaa tagacttgga agcctggacg ctctgtgatc cagtcttcca gtacacattc
      781 taaacgacca gtatcaaggt actccttgat ctgttcatag agcagatacc cgataccagc
      841 acccatcaaa acggcctcca gttcagcatc aacatcatcc agaatgatat ttcctgcgac
      901 agcaatttcc agctcctttg caaattgcca gtggaaaggt ttgccgctgg gataacgaaa
      961 aacaacacat tgatgattaa gcagatcatg aggatggcgg ggtttgccat atcttgcaaa
     1021 gtactctggc gttgctgcta cacataattt cactggtggg ccgatcgcca cgctgatcat
     1081 atctttttcg acgatgccac taagtcgaac gccagcgtca aagccctgtt ggacgatatc
     1141 gataagagaa tcgtcagttg tgagctctac tttaatatca ggatattcac gtgtgaaacc
     1201 aaccagtaaa ggcattaaaa atatacgtgc agctacccgg gcggcattga ttttgagcgt
     1261 tcctgtcggt gtcagacgaa aatcattcat ctcatctaac atgatctgaa tttcgtcgaa
     1321 agctgggcgc aatcgttcat agagattaga acccgcttcc gttaacgata cgcttcgggt
     1381 tgtccgattg aataagcgaa t
//
LOCUS       DADMEQ010000278         1395 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.280, whole genome shotgun sequence.
ACCESSION   DADMEQ010000278 DADMEQ010000000
VERSION     DADMEQ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.280"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..171)
                     /locus_tag="JNZ05_004151"
     CDS             complement(<1..171)
                     /locus_tag="JNZ05_004151"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567106.1"
                     /translation="MALPFAADACLVASSFEADADDADDAAEPADVLAARAEPAASVA
                     LVAASLADVPASL"
     gene            308..>1395
                     /locus_tag="JNZ05_004152"
     CDS             308..>1395
                     /locus_tag="JNZ05_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8567107.1"
                     /translation="MEDASTTKKGIVQLSSATNSASESLAATAKAVKVVMDETNKKAP
                     LNSPALTGTPTTPTAPKGTNNTQIASTAYVMAAIAALVDSSPDALNTLNELAAALGND
                     PNFATTMTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAK
                     STVAAVIEYLGLQETVNKADNAVQKTGDTLSGGLTFENDSILAWIRNTDWAKIGFKND
                     SDADTDSYMWFETGDNGNEYFKWRHRLAGGQLKELMNLKWDSLNILVNAVINGCLGIG
                     TTNALGGNSIAFGDNDTGLKQNGDGLLDVYANGQHVFRFQNGVAIAFKNIQAGTARKF
                     TLSSANNSTKNAAFYLWGNPSRPVVAELG"
BASE COUNT          365 a          344 c          398 g          288 t
ORIGIN      
        1 cagggatgcc ggcacatctg ccagtgaggc ggcaaccaat gcgactgatg ctgcaggctc
       61 agcacgcgca gccagcacgt cagccggttc ggcagcgtca tcggcatcat ctgcgtctgc
      121 ttcaaaagat gaggcgacta aacaggcgtc agcagcgaag ggcagtgcca cgacggcatc
      181 ctctaaggcg acagagtctg caggcagtgc gacggcggca gcgcagagca aaagcgcggc
      241 agaatccgcg gcaatgcgcg ctgagacagc agcaaaacgg gcagaggata ttgctgatgt
      301 cgtatctctg gaagatgcga gcacgacgaa aaaggggata gtgcagctca gcagtgcgac
      361 caacagtgca tccgagtcac tggccgcgac agcgaaagcg gttaaggtgg taatggatga
      421 aacaaacaag aaagcgcccc tgaacagtcc ggctctgacc ggaacgccaa ccacacccac
      481 tgcgccaaag gggactaata atactcagat cgccagtacg gcttatgtta tggccgcgat
      541 tgctgccctc gtggactcgt cgcctgatgc actgaacacg ctgaatgaac tggcggcggc
      601 gctgggcaac gacccgaatt ttgcgaccac catgactaac gcgcttgcgg gtaagcaacc
      661 gaaagatgcc actctgacgg cgctggcggg gcttgctact gcggcagaca ggtttccgta
      721 ttttacgggg aatgatgtcg ccagcctggc aaccctgaca aaagtcgggc gggatattct
      781 tgcgaaatcg accgttgccg ccgttatcga atacctcggt ttacaggaaa cggtaaacaa
      841 ggctgataac gctgtccaaa agacaggcga taccttgtcc ggtggactta cttttgaaaa
      901 cgactcaatc cttgcctgga ttcggaatac tgactgggcg aagattggat ttaaaaatga
      961 ttcggatgca gacaccgatt cctacatgtg gtttgaaaca ggtgacaacg gcaatgagta
     1021 tttcaaatgg agacaccgcc tcgctggcgg ccagctcaaa gagctgatga atcttaaatg
     1081 ggattcacta aatattctgg ttaatgccgt cattaatggt tgccttggca ttggtacgac
     1141 gaatgcgtta ggtggaaatt caattgcttt cggggataac gataccggcc tgaaacagaa
     1201 cggcgatggt ctgctggatg tttatgcgaa tggacagcat gtatttcgtt ttcagaatgg
     1261 cgtggctatt gcttttaaga atattcaggc cggaactgcc agaaaattca cgttatccag
     1321 cgccaacaac tccacgaaaa atgcagcgtt ttatttgtgg ggtaatccat ccaggcctgt
     1381 tgttgcagag cttgg
//
LOCUS       DADMEQ010000279         1387 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.281, whole genome shotgun sequence.
ACCESSION   DADMEQ010000279 DADMEQ010000000
VERSION     DADMEQ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.281"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(1..573)
                     /locus_tag="JNZ05_004153"
                     /pseudo
     CDS             complement(1..573)
                     /locus_tag="JNZ05_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(621..1115)
                     /locus_tag="JNZ05_004154"
     CDS             complement(621..1115)
                     /locus_tag="JNZ05_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8567108.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTGQAIAATGV
                     GIRIFNQSDNSQVKLYNDSDYTAIDAEGKAEMKFIARYVATNATVTAGTANADSQFTV
                     EYKK"
BASE COUNT          423 a          280 c          261 g          423 t
ORIGIN      
        1 catcacactg atatgaattg ggttgggatt ctctgctatc agatttcgcc cttcccggcg
       61 ccatttcact ttcggcgcag catcttcggg aataaggcgg atatttgctg gtcgccagaa
      121 cagcttgata cgcgttttta ctgctatttc cagcgagttc gtcgctttgt tgctcgctga
      181 tggcggaata tttttaatat tcagccagaa gactgactcg cgatcatcag gcaatgacac
      241 acctttggtg tgaataatgc gtaatatttt ttcttgcgaa ggttctaatc gtgatactgg
      301 cggaataacg gtaaacggcg ctttgctttt atcctcttta ttaaatggat ctacccaagc
      361 ctggatgaga taaggtattt tttcttcttt gctaaataca gaaatagatt tatcagcatt
      421 attacttaag taaaccaccc gcgtactacc aatgacaacg cctgcataag ttaatgctgg
      481 ttggcctaaa aaaacgctga gtataatcgc ggtaaataac ttacattaaa ctgttgtttt
      541 aaagagcctg attaaacaac tttttctgga cataacgact ccctggccaa agccaattga
      601 ataaaaaggc ccttgataaa ttatttctta tattcgacag taaattgtga atcagcgttc
      661 gccgtaccag ccgttacggt cgcattggtt gccacatagc gtgcaataaa cttcatttca
      721 gccttgcctt cagcatcgat agcggtataa tcagagtcgt tataaagttt aacctgcgaa
      781 ttatcggact ggttaaaaat acgaatacca acgccggttg ccgcaatcgc ttgtcctgta
      841 ccatcaaaac gaacggctgc ttgagtgtac gttgccgggc aattttcaag tttaatactg
      901 aacttctgtg gcgacgccag actacctaca cctgaaaatg catttttact gactttaccc
      961 agtgggacaa tttgatcggc tgtatcggag gtaacttcac aagtagtgtc gacaatttca
     1021 ccgacgaagt taacaacacc atcttctgcc agtgcatgcc cggccagcat tagagatgcc
     1081 agagtgaatg caccgcctat aatattacgc ttcataaaaa ctccattgat ataatatatc
     1141 ctttaccatg aatcatcact tatattaata agtgtatttt tgattcaaag gaatagtaca
     1201 ccaaagagac ggctttatct aaaaataata ttaattacgc aaaaataaaa tatagattta
     1261 ttgtttaaat attatttaaa cagtaaaaaa tcaagtttgt tatcagacat taatgataat
     1321 tcagaatata tttatataga aagatagatg aatttattga tgtcagttat aaaaaagggc
     1381 caccggt
//
LOCUS       DADMEQ010000280         1345 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.282, whole genome shotgun sequence.
ACCESSION   DADMEQ010000280 DADMEQ010000000
VERSION     DADMEQ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.282"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            443..1192
                     /locus_tag="JNZ05_004155"
     CDS             443..1192
                     /locus_tag="JNZ05_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY8567109.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          376 a          274 c          279 g          416 t
ORIGIN      
        1 gagtggtgga ttagcccggc aggtgggcgc tggctcctga gcaccataaa cagggcttta
       61 cccgtaaata ttggctcctt ctgatgccat tctatttcag gaaggagtgt ccgttaaact
      121 caggctacct caaaaagtta tcggtgcgtt tatcgaaaaa catatgttct actaattgga
      181 gtcattgtct gtaaaagcaa ctcaaaaata caagttgctt attccccttg ttggtaagaa
      241 cttaattcta actgtttgtt ctggatgttt taaacgtcct cctttttatg cttgttcata
      301 acggatattt aataggtaac ctttgtttta gcatcttctg atttatttaa attatgtgat
      361 agcgcacaca attttattat ccagtacttt atactcttaa taaaataatt ataaatattt
      421 atcctctata aggagatgtt atatgaaaag acagcttttt actcttagta ttgtcggggt
      481 attttcttta aatacctttg catcattccc tccgggaaat gatgtgacaa caaaacctga
      541 cctttactac ctgacaaacg ataatgctat tgacagtctg gcattattac cgcctccacc
      601 acaaatcgga agtattgcct tcctgaatga ccaggccatg tatgaaaagg ggcgtttatt
      661 gcgaaatact gaaagaggga agctggcagc tgaggatgcc aatctcagta gtggtggcgt
      721 ggcaaatgta ttttctgctg cttttggttc tccgataacg gctaaagatt caccagaatt
      781 gcataaatta ctgacaaata tgattgagga tgccggagac ctggcaacgc gttcagcaaa
      841 agaatattat atgcgtattc gaccttttgc tttttatggt gtttctacgt gtaatacaaa
      901 agaacaggat acattatcca gaaatggctc ttatccatca ggtcatacat cgattggttg
      961 ggcaacagca cttgtattgt ccgaaataaa tccggcacgc caggatacca ttctgaaacg
     1021 gggttatgaa ctgggggaca gcagggttat ttgcggttat cactggcaaa gtgatgttga
     1081 cgcagcacgt attgttggct ctgctattgt agcaacactg cactcaaatc ctgtgttcca
     1141 ggcacaatta caaaaagcga aagatgaatt cgctaacaat cagaaaaaat aatgctttta
     1201 gccagtacgc cttataaccc cgaccttcag ggctaatgcc agtcagttaa gcaactgact
     1261 ggctcttttt cagggctgtg gggtatttcc agggcctctc ctttaccact ctcgggaagg
     1321 ccctttccct tcttgtcggt aattt
//
LOCUS       DADMEQ010000281         1342 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.283, whole genome shotgun sequence.
ACCESSION   DADMEQ010000281 DADMEQ010000000
VERSION     DADMEQ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.283"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(19..501)
                     /locus_tag="JNZ05_004156"
     CDS             complement(19..501)
                     /locus_tag="JNZ05_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY8567110.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDNARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(492..794)
                     /locus_tag="JNZ05_004157"
     CDS             complement(492..794)
                     /locus_tag="JNZ05_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY8567111.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1121..1339
                     /locus_tag="JNZ05_004158"
     CDS             1121..1339
                     /locus_tag="JNZ05_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567112.1"
                     /translation="MKAVARANNHPYKSVFADFITGHPSCTVCFWETFHKMYPDSPYE
                     YVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          396 a          333 c          286 g          327 t
ORIGIN      
        1 tcagactcag actcagactc agactttata aaacaaggta ttaggtgcca caactgactg
       61 aacaaaacca aaacgctcat aaaatgctct ggcgttatca tcaattgcat gtaccataac
      121 agccttaatg ccaacctgat cagccagatt gacaacccgg tgaatggcat cacttagtag
      181 ccatttaccg aaaccatgcc cctgggtaca aacatcaaca gccagacgcc ctaacagaac
      241 aacagggaca ggattgggca tgttatgccg caaactgcga ccaagctcgg catgagtgac
      301 ggaaccagta gctaatgagt agtatcccac aatacgtaaa gtatcctcca agcaaataac
      361 gaacgtgcga gaagcattga gcatctggtt tttcatagcc cgaccacgta accagttact
      421 tagcacctca tttccacagt caaatggctg aagtatatgc tcatccgtca acaatgctgg
      481 cgcggtgaca tttatttcca ttcaggcttt accgccatca gacgttcacg cccttctgca
      541 ttctgcacag gagcttccag ttgcgtaata aacgaatcat aacgttcgtt atccagcaca
      601 aataaacgtt gatccaggat aatgttttta gcttcctggt acgccagttc catgatgaaa
      661 tcggtacggt tcttattgac caagctcgca gcataatcaa taacggctcg ttcctcatca
      721 gttgcccgga tgttaatctg atgttctcgc ggtgttttac ggacgcttgc agctgtagac
      781 ataaaaacct ccattggtaa caatttgcta tgcaattaat gttatcgcaa atggtaacag
      841 aacgctatac agaatcactt acgggaaaag cgatcccctg ataaaaacaa tggcagagac
      901 ctgaacattg tgacgatgat ctgtgtcggc gcacaaatca tgtctgatta cggccgttta
      961 atgtgatttt gataatgaaa tgcctgaacc acggcagcct ggcggctgca aatcggggtg
     1021 tggcagaata tgagctttca gagtaaccat ccagaatgag ttcccatgtc acacaacctg
     1081 gagcaccaga aagtccacac cagaatggtg aaagagggtt ctgaaagccg tcgccagagc
     1141 caataatcac ccttataagt ccgtatttgc agactttatc accggtcatc cctcctgcac
     1201 ggtctgtttc tgggaaacct tccacaaaat gtacccggat tcaccgtacg aatacgtcac
     1261 gttctgccat acctgccgtc gttttgattt atacgaaaca gaagcggaga tgaaggcgga
     1321 tgacccgaag tggtggtgat tt
//
LOCUS       DADMEQ010000282         1296 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.284, whole genome shotgun sequence.
ACCESSION   DADMEQ010000282 DADMEQ010000000
VERSION     DADMEQ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.284"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..815)
                     /locus_tag="JNZ05_004159"
     CDS             complement(<1..815)
                     /locus_tag="JNZ05_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY8567113.1"
                     /translation="MKPVHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="JNZ05_004160"
                     /pseudo
     CDS             907..993
                     /locus_tag="JNZ05_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1003..1083)
                     /locus_tag="JNZ05_004161"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="JNZ05_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          363 a          219 c          336 g          378 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtggacaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaaatg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt cagctt
//
LOCUS       DADMEQ010000283         1277 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.285, whole genome shotgun sequence.
ACCESSION   DADMEQ010000283 DADMEQ010000000
VERSION     DADMEQ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.285"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="JNZ05_004162"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="JNZ05_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HAY8567114.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          359 a          230 c          284 g          404 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttatt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt agtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaccaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DADMEQ010000284         1227 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.286, whole genome shotgun sequence.
ACCESSION   DADMEQ010000284 DADMEQ010000000
VERSION     DADMEQ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.286"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..884
                     /locus_tag="JNZ05_004163"
     CDS             <1..884
                     /locus_tag="JNZ05_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY8567115.1"
                     /translation="PTISLSAVIYFPPKFDETRQYPAIVVSHPGGGVKEQTAGTYAEK
                     LAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTTLSYVDNTRIGAMG
                     ICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSIDALPHIEAGSNART
                     SDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPGYATLRSLNQIITY
                     DAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIVEGANHMDLYDGKA
                     YVAEAISVLAPFFEETL"
     gene            884..>1227
                     /locus_tag="JNZ05_004164"
     CDS             884..>1227
                     /locus_tag="JNZ05_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY8567116.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGV"
BASE COUNT          325 a          280 c          317 g          305 t
ORIGIN      
        1 atccaaccat ctctttgtct gcagtgatct atttccctcc aaaatttgat gaaacccgtc
       61 agtatccggc aattgtggta tctcacccag gtgggggcgt taaagagcaa acggccggaa
      121 cctatgccga aaagctggcg gaaaagggat ttgtcactat tgcttatgac gcatcttatc
      181 aaggtgaaag tggcggcgag ccgcgtcagt tagaaaaccc atacattcgt actgaagacg
      241 tcagtgccgt gattgattac ctgacaacgc tttcttatgt cgacaatacc cgaattggtg
      301 cgatgggaat ttgtgccggt gcaggatata cagccaatgc tgcaattcag gatcgccgca
      361 tcaaggccat cggtaccgtt agtgcggtca atattggttc gatgttccgc aatggttggg
      421 aaaacaatgt gaaatctatt gatgctctgc cacacattga agctggctca aacgcccgaa
      481 ccagcgatat tagcagcgaa gaatacgcca ttatgccact ggctccaatg aaggaggccg
      541 atgcgccgaa cgaagaactg cgtcaggcct gggagtatta ccacacacct cgtgcgcagt
      601 atccaacagc accgggttac gctactttgc gcagccttaa ccagattatt acctatgatg
      661 cttaccatat ggcggaagtg tacctgactc agccgatgca gattgtggcg ggcagccagg
      721 cagggagcaa atggatgagt gacgacctgt acgatcgggc atcaagccag gataaacgct
      781 atcacattgt tgaaggcgca aaccatatgg atctttatga cggcaaagcc tatgtggctg
      841 aagcaatttc agtattagct ccgttctttg aggaaactct gtaatgaaac atattcaaat
      901 ccgcaatagt gatatggcct ggcatattgc tgccaatatt cagtttccac caaattttga
      961 tgagtcaaaa cagtatccgg cgattatcag cgtccatcct tttggcagtt gtaaggagca
     1021 gacgtctggc aatatctatg gcaaggcatt ggctgaaaaa ggctatgtcg ttctcgctta
     1081 tgacgccagt ttccagggag aatctggtgg ggagcctcgt tggatagaag atccgacaca
     1141 gcgcgtagag gatattagcc gtgttatcga ttatgcagta actctgccat atgttgatgc
     1201 tgaacgcatt ggcgtgctgg gtgtgtg
//
LOCUS       DADMEQ010000285         1210 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.287, whole genome shotgun sequence.
ACCESSION   DADMEQ010000285 DADMEQ010000000
VERSION     DADMEQ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.287"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(13..753)
                     /gene="cysQ"
                     /locus_tag="JNZ05_004165"
     CDS             complement(13..753)
                     /gene="cysQ"
                     /locus_tag="JNZ05_004165"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HAY8567117.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            943..>1210
                     /locus_tag="JNZ05_004166"
     CDS             943..>1210
                     /locus_tag="JNZ05_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567118.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSA"
BASE COUNT          280 a          327 c          297 g          306 t
ORIGIN      
        1 tgccatctga atttagtaaa tggacactct gaaccccgga ttcaggaacg actcgcgcgg
       61 agtgtaatcc agcggtttac cctgccagtc gtgaacgtgc gctccggcag ctgcagccac
      121 agcatgtcca gcggcggtgt cccaaatatt cgttggtccg aagcgcgggt acagctgcgc
      181 ctgtccttcc gccaccaggc agaatttcag cgaagagccg atggacgtgg tctgatgttc
      241 gccaagctgt tgcagatact ctttcagctc cgcatctgca tgggaacggc tgataaccac
      301 cagcggcggg cgcgcatcgc ggacctgaat cagcttgcgc acaccgcact cttctttcca
      361 ggccttgcct tctgccgcgc tgtacattac gttcattacc ggcgcataca ccacacctaa
      421 aatcggtttg ccatggtcaa tgagcgcaat gttaacggtg aattcgccat tacgtttaat
      481 aaactcttta gtaccatcca gcgggtctac cagccagtaa cgctgccagt gctgacggac
      541 ttcccaaccg ggaggatctt cttcagaaag gaccggaata tccggtgcca gcgtacgtaa
      601 accgtccatg ataacggtgt gagcggcaat atccgctgcc gttaccggag aattgtccgc
      661 tttgctgacg acgtccatcg gtttcgtccc gtcgtagacc tgcataatgg catcgcctgc
      721 attccgtgca agctggcata cttgatctaa catttctcca cctcgtctct gtgagcggtg
      781 ttaacttatt gttttactta taccctatcg ttaatgaatg cgccaactgt gatagtgtca
      841 tcattttcaa agcgtaaaat tgtggcattc ttcactgttc tataagtaag gcgtttattc
      901 tcccttttct ttcgtattcc cgatgataaa aggatgtccc tgatgattaa gtttagcgca
      961 acgctcctgg ccacgctgat tgccgccagt gtgaatgcag cgacggtcga tctgcgtatc
     1021 atggaaacca ctgatctgca tagcaacatg atggatttcg attattacaa agacaccgcc
     1081 acggaaaaat tcggactggt acgtacggca agcctgatta acgatgcccg caatgaagtg
     1141 aaaaacagcg tactggtcga taacggcgat ttgattcagg ggagtccgct ggccgattac
     1201 atgtcggcga
//
LOCUS       DADMEQ010000286         1207 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.288, whole genome shotgun sequence.
ACCESSION   DADMEQ010000286 DADMEQ010000000
VERSION     DADMEQ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.288"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            14..925
                     /locus_tag="JNZ05_004167"
     CDS             14..925
                     /locus_tag="JNZ05_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY8567119.1"
                     /translation="MINEGDKSQEDIADEAGMSPATLSALRKGEYNGNVEGMHDKLQK
                     WYQVWQKGQNLSGTPGVVKTQIFCDLHELFKTVRSLGIISVVVGVPGVGKTFAAREYC
                     RRTANAWMVTLAPAHSSVTECLLEIAYALEINNPGRNKGNITRAIRRKLGGGKETSGR
                     VNPLLIIDEADHLSVDGLEQLRAIQDATGVGMVLIGNPKQMADATRRGTDELARLFSR
                     FAMTKQLRKVKKTDVMAVAKAWGVTQEDELDLLMRVAEKPGGLRVLTHTLNHAWLAAK
                     GAGTSLNKSHIKAAFKEAYSNPRLLNW"
     gene            941..1168
                     /locus_tag="JNZ05_004168"
     CDS             941..1168
                     /locus_tag="JNZ05_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567120.1"
                     /translation="MMSRNIKMATDAQNWLQAHGSHVNESYLGVARPVLEITCPPPEL
                     VRNAVRIMEHKSGVARSVWTARLNGCQIIWR"
BASE COUNT          340 a          256 c          336 g          275 t
ORIGIN      
        1 cactattaac gctttgatta acgagggcga caagtctcag gaagatattg ctgatgaagc
       61 cggaatgtct ccggcaacac tctcggctct gcgtaaagga gagtacaacg gtaatgtcga
      121 aggtatgcat gacaaattac agaaatggta tcaggtctgg cagaagggac agaacttgtc
      181 aggtacccct ggtgttgtga agacccagat attttgtgat ttgcatgagc tgtttaaaac
      241 agtgcgctca ctcgggatta tcagtgtcgt tgttggcgta ccgggtgtcg gaaaaacctt
      301 tgctgcccgg gaatattgtc gcaggacagc taatgcctgg atggtgactc ttgctccggc
      361 acattcgagc gtaacggaat gcctccttga aatagcatat gcgctggaaa ttaataatcc
      421 gggaagaaat aaggggaata taacccgcgc cattcgcaga aaactgggtg gtggtaaaga
      481 aaccagtggc cgtgttaacc cccttctgat tattgatgaa gctgatcact taagtgtgga
      541 tggtctggag caattacgcg ccattcagga tgccactggc gtggggatgg ttttaattgg
      601 caacccgaaa cagatggcgg atgccacccg tcgtgggact gatgaactgg cccgtctgtt
      661 cagccgtttt gccatgacaa aacaactgcg caaagtaaaa aagaccgatg tgatggctgt
      721 agccaaagca tggggagtaa cacaggaaga tgagctggac ctgctgatga gggtagccga
      781 aaagcccggg ggattgcgtg tattaactca cacactgaat catgcgtggc tggcggcaaa
      841 aggtgcaggc acatccctga ataaaagtca cattaaagcg gcttttaagg aggcttacag
      901 caaccccagg ttactgaact ggtagtaaag gacataaacc atgatgtcac gaaatataaa
      961 aatggcaacg gatgcgcaga actggttaca ggcgcacggg agtcacgtaa atgaatcata
     1021 tctcggcgtg gcgcgtccgg tacttgaaat cacctgccca ccaccggagc tggtcagaaa
     1081 cgctgtcagg attatggagc ataaatcagg ggttgcccgt tcagtatgga cggcccgcct
     1141 taatggctgc cagattatct ggcgttaata acggcttatt tatttgtgag gaataacaaa
     1201 atggcaa
//
LOCUS       DADMEQ010000287         1191 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.289, whole genome shotgun sequence.
ACCESSION   DADMEQ010000287 DADMEQ010000000
VERSION     DADMEQ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.289"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            75..1100
                     /locus_tag="JNZ05_004169"
                     /pseudo
     CDS             75..1100
                     /locus_tag="JNZ05_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            1073..>1191
                     /locus_tag="JNZ05_004170"
     CDS             1073..>1191
                     /locus_tag="JNZ05_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HAY8567121.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQV"
BASE COUNT          333 a          293 c          272 g          293 t
ORIGIN      
        1 taacgcttac cgctcatggg cacctctctt taagccatct taaatgactc tgaggtgtct
       61 gttaaacccg tggcgattca caattctctt gcaccattaa ctatgttcag tccctgcgcg
      121 agttgtacac cgatgtcatc gaagagtttt actgggttgc gctcccgctt acgacgcaaa
      181 attccctttc acaataccaa cccgaatggc agtgctgaga acctgatgtc gaatgggtgc
      241 gtcagccccc gcaagatgcg ataaccgctc ctgacttttt ctgcttttac cagccaggca
      301 tgaccttcga acaatttgta cgtgagtttg ccgaatggtt ttcacaaaaa cgtccggcgg
      361 cgatgatgat cggcatccgt gcggatgagt cctacaaccg ttttgtcgcc atcgccagtt
      421 taaataaaca acgttttgcc gacgataaac catggactac ggccgcacca ggcggtcata
      481 gctggtacat ttaccccatt tacgactgga aagtggctga tatctggacc tgatatgcaa
      541 atcatcaaaa tctctgcaac ccactgtata acctaatgta tcaggcaggc gttcctctgc
      601 gtcatatgcg aatttgcgaa ccttttggcc cggagcaacg acaaggatta tggctctatc
      661 acgttatcga accggatcgc tgggctgcta tgtgcgcacg agtcagcggg gtaaaaagtg
      721 gcggcattta cgccggacat gacaatcatt tctatgggca tcggaaaatc ctcaaaccag
      781 aacatttaga ctggcaagaa tatgcattat tgctgctcaa tagcatgccg gaaaaaacag
      841 ctgagcatta ccgcaataaa atcgccattt atttgcactg gtatcagaaa aaaggcatcg
      901 aggttccaca aacccagcaa ggggacattg gcgcgaaaga tattccctcc tggcggcgga
      961 tatgcaaagt tttactcaat aacgattact ggtgtcgggc attatcattt agtcctacga
     1021 aagcgaagaa ctatcagcat tataacgaac ggataaaagg aaaacgtcag gaatggggga
     1081 tactatgcaa caacgattaa cgcaggattt aacgcaattt ctcgccagct tgccggaaga
     1141 tgatcgcatc aaagcgatta atgaaatccg catggcgatc catcaggtta g
//
LOCUS       DADMEQ010000288         1168 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.290, whole genome shotgun sequence.
ACCESSION   DADMEQ010000288 DADMEQ010000000
VERSION     DADMEQ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.290"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(67..192)
                     /locus_tag="JNZ05_004171"
                     /pseudo
     CDS             complement(67..192)
                     /locus_tag="JNZ05_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(264..1133)
                     /locus_tag="JNZ05_004172"
     CDS             complement(264..1133)
                     /locus_tag="JNZ05_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HAY8567122.1"
                     /translation="MPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDDPFGAL
                     MQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETTDLPCW
                     RHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLWQDEHLLRLCLR
                     QALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVCYRGQV
                     HHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGALAVTAK
                     GAMTALPCRQELE"
BASE COUNT          305 a          336 c          247 g          280 t
ORIGIN      
        1 acctgtgttg ttctgaggtg agcatatcac ctctgttcag gtggccaaat tcagtgtgcc
       61 acttcaccct agatgtcctt tcagccaaat agcgtataac gaccaccaca gcgaccagga
      121 aataaaaaag agaaatgagt aactggatgc aaaacgatag tacgcatttc tgaatggaat
      181 attcagtgcc ataattacct gcctgtcgtt aaaaaattca cgtcctattt agagataaga
      241 gcgacttcgc cgtttacttc tcactattcc agttcttgtc gacatggcag cgctgtcatt
      301 gcccctttcg ccgttactgc aagcgctccg caacgttgag cgagatcgat aattcgtcgc
      361 atttctctct catctgtaga taatcccgta gaggacagac ctgtgagtaa cccggcaacg
      421 aacgcatctc ccgcccccgt gctatcgaca caattcacag acattccagc aaaatggtga
      481 acttgtcctc gataacagac caccacccct tctgcacctt tagtcaccaa cagcatggcg
      541 atctcatact cttttgccag ggcgcatata tcctgatcgt tctgtgtttt tccactgata
      601 agtcgccatt cttcttccga gagcttgacg acatccgcca gttgtagcgc ctgccgcaaa
      661 cacaagcgga gcaaatgctc gtcttgccat agatcttcac gaatattagg atcgaagctg
      721 acaaaacctc cggcatgccg gatcgccgtc atcgcagtaa atgcgctggt acgcgaaggc
      781 tcggcagaca acgcaattga acagagatgt aaccattcgc catgtcgcca gcagggcaag
      841 tctgtcgtct ctaaaaaaag atcggcactg gggcggacca taaacgtaaa tgaacgttcc
      901 ccttgatcgt tcagatcgac aagcaccgtg gatgtccggt gccattcatc ttgcttcaga
      961 tacgtgatat cgactccctc agttagcagc gttctttgca ttaacgcccc aaaaggatca
     1021 tcaccgaccc gacctataaa cccacttatt ccgcctaatc tggcgattcc caccgcaacg
     1081 ttagctggcg cgccgccagg acaaggcagt aggcgcccgt ctgattctgg caagagatct
     1141 acgaccgcat cccctaaaac ccatactt
//
LOCUS       DADMEQ010000289         1155 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.291, whole genome shotgun sequence.
ACCESSION   DADMEQ010000289 DADMEQ010000000
VERSION     DADMEQ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.291"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(569..748)
                     /locus_tag="JNZ05_004173"
                     /pseudo
     CDS             complement(569..748)
                     /locus_tag="JNZ05_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            842..>1155
                     /locus_tag="JNZ05_004174"
     CDS             842..>1155
                     /locus_tag="JNZ05_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY8567123.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGR"
BASE COUNT          318 a          213 c          298 g          326 t
ORIGIN      
        1 tagggtagtc actatatttg cgactgagct ggtcgctatt tttaaccagg ctgaaaaatt
       61 gagtcaatat atcgaaagcg gtagttggcg ggcataagta tgtaatgatg attctgcact
      121 ggtgttgaat attattcacg ccaaactttc ctcattgagc aatgtcgtcg caagtggcct
      181 caatgatttt agaccattat cagaggtccg gagcggcggg aagagattta ttgctgtgga
      241 ataattgtgg tactgatgat tatttatatc ccggattggt agggaaaagc ttttttcatg
      301 cactattttt gcaaatattt ctatatggga agcggttttg ttacagatta aatttataaa
      361 gaaatgcatt tggggtgttg ggttgaagag tagctggtag atgttattgt gccagcaggg
      421 gcaggggaat gtgccgtatc gatatgcttt ggcaattgcg tgtgccgcag atggagatga
      481 aatgccaata aaaaagcctg agttcgatga gggacgaact caggctggtg ttatgtttta
      541 cagtaacacg cctcggcgcg ttggctgatt agaactggta gacgatacca actgccagtt
      601 ggtcgtcggt gttcagtcca tgagcgagaa cataatcact cttatcaagc aggttgatct
      661 gataagcggt atatatgttc atattcttat tgaagtaata ccaggtaccg atttcaatat
      721 agttcagacg atcagaatct ttgtaacctg tagactggcc ccctgaatct ccagacaacc
      781 aatatcactt atttaagtga tagtcttaat actagttttt agactagtca ttggagagca
      841 gatgattgat gtcttaggac cggagaaacg cagacggcgt actacacagg aaaagatcgc
      901 tattgttcag cagagttttg aaccgggaat gacggtctcc cttgttgccc ggcaacacgg
      961 tgtggcagcc agccagttat ttctctggcg caagcaatac caggagggaa gtcttactgc
     1021 tgtggccgca ggagaacagg tcgttcctgc ctctgaactt gctgctgcca tgaagcagat
     1081 taaagaactc cagcgcctgc tcggcaaaaa aacgatggaa aatgaactcc ttaaagaagc
     1141 cgttgaatat gggcg
//
LOCUS       DADMEQ010000290         1145 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.292, whole genome shotgun sequence.
ACCESSION   DADMEQ010000290 DADMEQ010000000
VERSION     DADMEQ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.292"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..679
                     /locus_tag="JNZ05_004175"
     CDS             <1..679
                     /locus_tag="JNZ05_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8567124.1"
                     /translation="CEAHSHRSVALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSG
                     SPDIEMIKNRMAELFNQSDISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILAT
                     AINETLEGDTNVAVVMGCKSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGY
                     FSCDSLTPEELMMLKNLFDIRVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNS
                     YTVGMGV"
BASE COUNT          338 a          226 c          266 g          315 t
ORIGIN      
        1 ctgtgaagcg catagccatc gcagcgtcgc ccttgccaaa accgtggctg cgcccatcat
       61 ggcgacaggt gtgggcttgg cattaatgta tgcaacatca tcaggaccgt attatgcgca
      121 aagtctttct ggttcgccgg atatcgagat gataaaaaat cgcatggctg aattgtttaa
      181 ccagtcggat atttctgtgc gcaacaaata tagcccggct tttgataaat ggaatgaact
      241 ggtagataaa ttaaatcacg aaagaaatac agttccgttt gcgtgtttga ccaccatttt
      301 agcgacggcg ataaatgaaa ctcttgaggg ggatacgaat gttgccgttg tgatggggtg
      361 taaatctgcg aaagatcgca cgatatccat tgtccttggc aactcgatgt tgcaaacact
      421 ccttgaaaaa cgcctcgccg atagtggaga aatagaaaat ctttttgatc aacagggata
      481 ttttagttgc gattcattaa cacccgaaga attaatgatg ttaaaaaacc tttttgatat
      541 tcgagttctt catgtctcta acaaattcaa tgttggattg caaggtaata tcaacactga
      601 tgttttacaa gattcgtttt ttaaaaatgt cgatttcatt catgaatata actcgtatac
      661 cgtagggatg ggagtctgac gagtaaaact cattaggatt atcagaattc ggacaatgca
      721 ttgcaaaaat gacatacaaa gagagatgtg tgcaggtact tagccgcgag gggcatggcg
      781 aagccataca gaaagtacca gtattcagaa ctccagtgaa agcggcatag tggccgcctg
      841 ctcaggagtt ttgctatgac ccgtaatcgt gctgaacgtc gccaccctat gatgcgatta
      901 aaaaaactca tcgtcgcgct gatgattgac gtcaatgcta tcggatgatg ccgggggtgt
      961 tccggaagga attccttgtt tcccgaactg ttaccaacaa aattataacg atgagcaagt
     1021 aaagaccggt tggtggcagt actgaccggt ctgtgatgaa agattaattt caccttaaat
     1081 cctggtgctg ttaggatgct gacgtttttc ggctacacca ttcagattat gcaaataaat
     1141 atccc
//
LOCUS       DADMEQ010000291         1126 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.293, whole genome shotgun sequence.
ACCESSION   DADMEQ010000291 DADMEQ010000000
VERSION     DADMEQ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.293"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            252..1007
                     /gene="mlaA"
                     /locus_tag="JNZ05_004176"
     CDS             252..1007
                     /gene="mlaA"
                     /locus_tag="JNZ05_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HAY8567125.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          276 a          260 c          293 g          297 t
ORIGIN      
        1 gcttttttgc tcgcaactgg cgggagttac tctgaaaatg tagaaaaggt tgcgtttgcc
       61 tttttctgtt tctatagaat caagtagcct acagggcggc gattaccagg ctatgatcaa
      121 atcagcaaat cagggcgtct ggacatcagt tgacgtgctg ttacaatcgc ccacacctaa
      181 acaggcggat acggtatcgt tccgtcatgg atggcaaact gcataagcca taaaaaaaca
      241 gggagacatt tatgaagctt cgcctgtcgg cgcttgctct gggaactacg cttctggtgg
      301 ggtgtgcgag ttccggtaca gatcagcaag ggcgttctga cccgttagaa gggttcaacc
      361 gcaccatgta caacttcaac ttcaacgtat tagacccgta tattgttcga ccggtcgctg
      421 tcgcctggcg tgattatgtt ccgcaaccgg cgcgtaatgg tttgagcaac tttactggca
      481 accttgaaga acctgcggtg atggttaact acttcttgca gggcgaccct tatcagggga
      541 tggtccactt tacccgcttt ttcctgaaca ccattttggg gatgggcggc tttattgatg
      601 ttgcagggat ggcgaacccg aaactgcaac ggactgaacc tcaccgcttc ggtagtacgc
      661 ttggtcatta tggcgtgggt tatgggcctt acgttcagtt accgttctac ggtagcttca
      721 cgctgcgtga tgacggtggt gatatggcgg atggtcttta cccggttctt tcctggctga
      781 cctggccgat gtctgtgggt aaatggacgc ttgaagggat cgaaacccgc gctcagctgc
      841 tggattccga tggtctgctg cgtcagtcgt ccgatcctta tattatggtg cgcgaagcgt
      901 acttccagcg tcatgatttc atcgctaatg gcggcgaact caaaccgcag gaaaatccga
      961 acgcgcaagc gattcaggat gatttaaaag atatcgattc tgaataagaa acaaataaaa
     1021 aaggtgagtc gcaaaactca ccttttttgt tgttatccct caattaccac aagtaaaagg
     1081 agataaaccg acaatgaccc ggacgtaatg atgtaaaaat actgat
//
LOCUS       DADMEQ010000292         1121 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.294, whole genome shotgun sequence.
ACCESSION   DADMEQ010000292 DADMEQ010000000
VERSION     DADMEQ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.294"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..>1121
                     /gene="pdeF"
                     /locus_tag="JNZ05_004177"
     CDS             <1..>1121
                     /gene="pdeF"
                     /locus_tag="JNZ05_004177"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAY8567126.1"
                     /translation="TYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVFLFYLP
                     LALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGGYRVFT
                     PRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMPFNLGT
                     LINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFALWLLAL
                     GALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMISIHSY
                     QNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDPVVHLP
                     NVRALNRALRDALWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT          245 a          254 c          251 g          371 t
ORIGIN      
        1 caacttatat aaaaatacgt gataaatggt gggggcttcc gctgttcctg ccttctttaa
       61 tcttgcccat tttcgcccac attaatactt tcgcgcatat ttcttccggt gaggtttttc
      121 tcttttatct gcctttggca ctgatgatca gcatgatgat gtttttcagc tgggcggcat
      181 tgccagggat caccttaggg atttttgtcc gcaaatatgc agagctgggt ttttacgaaa
      241 cgctctcatt aacggctaat tttattatca ttatcattct ctgttggggc ggttacaggg
      301 tctttactcc ccggcgtaac aacgtttcac atggtgatac ccgtttaatt tcccagcgta
      361 tattctggca gatagtgttt cctgcaacgc tgtttctgat acttttccag tttgctgcat
      421 ttgtaggatt actggcgagc agagaaaatc tggtcggtgt catgcctttt aacctcggga
      481 ctttaatcaa ttatcaggcc ttactggtgg gtaatctgat cggtgtcccg ctgtgctact
      541 tcatcattcg ggtagtgcga aatccatttt atttacgtag ctattattcg caattaaaac
      601 agcaggttga tgccaaagtc accaaaaaag agttcgcgct ctggctactg gcattaggtg
      661 ctttattgtt gctgttatgc atgccgttaa atgaaaaaag cacaattttt agcaccaatt
      721 ataccttgtc attattgctg cccctgatga tgtggggagc aatgcgctat ggttataagc
      781 tgatttcgct gctctgggcg gtcgtgttga tgatcagcat ccacagctat caaaattaca
      841 ttcccattta tcctggctat accacgcagc tgaccataac ctcctccagt tatctggtat
      901 tctcttttat tgtcaattat atggctgtac tggcaactcg tcagcgagcg gtagtcagac
      961 gcattcagcg gcttgcgtat gtggacccgg tggttcatct gccaaatgtt cgcgccctga
     1021 atcgcgcgtt gcgtgatgcc ctctggtctg cgctttgtta tttacgcatc cctggcatgg
     1081 aaatgctggt taagaactat ggcatcatgc tgcggattca a
//
LOCUS       DADMEQ010000293         1107 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.295, whole genome shotgun sequence.
ACCESSION   DADMEQ010000293 DADMEQ010000000
VERSION     DADMEQ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.295"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..387)
                     /locus_tag="JNZ05_004178"
     CDS             complement(<1..387)
                     /locus_tag="JNZ05_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY8567127.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNV"
     gene            816..1097
                     /locus_tag="JNZ05_004179"
     CDS             816..1097
                     /locus_tag="JNZ05_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567128.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          307 a          249 c          252 g          299 t
ORIGIN      
        1 aacgttaggt tcacgcttat aatcggccgg tgcaatcgta tagccgttta caggcgctat
       61 cagcgatgag cataaaaaat ggttatcacg ggcatagatc aattcattaa tataaagata
      121 gccacgaata atattcaaca ttcgcttttg atgggctgga gtgcaaactt gcccctgata
      181 gcgttcagct gcatcgctca ctaaatctgc ctggcgaatg accagctcgg atttatccag
      241 tgccagttga gcaaaggttc ggagctgatt attcacttct gattttgccc aaaggaaggc
      301 aatccatagc gaaagaatga tgggaaagag aactaaaaag ataatgcccg gcaacgccag
      361 taattggtgt cgtgcacgat gactcattgc tatcaatttc ctgcctgttt atacccggcc
      421 attgcaaggt gaaaccctgg ctaccgacgt ctttagataa atcttacggt ataaatagca
      481 cttttgcctg atttgaaatt gatacctttt taatcgtcac ctggatgaaa ataattcatt
      541 gatattcaaa attaattttc tacaggaata cagaggcggc gggaagatat gcccttaatg
      601 ccactgaaaa accacataat ctgtacagca ataaaagtca cggcctaata tggctaacaa
      661 acaatcaatt gtgatatggt tcacaaaatt aatgaaacaa acagagtgtt tcatttttgt
      721 gttctgcaag ccccgcgata gccagagtgt accacgcctc ccgtgaacaa cgccgcgctg
      781 tccagggcat cggctctttt cagaggaatt gtttgatggc taccctcacc actggcgtgg
      841 ttcttcttcg ctggcaactt cttagtgccg taatgatgtt tctggccagc acactcaaca
      901 tccgttttcg tcggtctgat tatgtcgggc ttgcggtgat cagcagcggt ctgggcgttg
      961 tttccgcctg ttggttcgca atggggttgc ttggcatcac aatggcggac atcaccgcta
     1021 tctggcacaa catcgagtcg gtgatgatag aagagatgaa tcagacaccg ccacaatggc
     1081 caatgatttt gacctgatac gtagaag
//
LOCUS       DADMEQ010000294         1089 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.296, whole genome shotgun sequence.
ACCESSION   DADMEQ010000294 DADMEQ010000000
VERSION     DADMEQ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.296"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..724
                     /locus_tag="JNZ05_004180"
     CDS             <1..724
                     /locus_tag="JNZ05_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY8567129.1"
                     /translation="YLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIIST
                     FAIDRREKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPSSYIEAAKEEGVTI
                     LSALSMLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSR
                     AFNHALSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPW
                     RSIGNLITLIVGILCVSVMFFS"
BASE COUNT          310 a          232 c          199 g          348 t
ORIGIN      
        1 ttaccttgtc ggtagttggc aacctgatct attaacaacc caggtagagt tcaatcagaa
       61 tacccttcac cagatatgga tatcgattcc cgtgatggtt ttcgccttta gccatacgcc
      121 cattatttct acgtttgcca tagacagacg tgaaaaatat ggcgaacacg ctatggataa
      181 atgcaaaaaa attatgaaag tcgcttatct catcatctgc ataagtgtac tgttctttgt
      241 ctttagctgc ctgctttcta ttccatcttc gtatattgaa gcggctaaag aagaaggggt
      301 caccatttta tcggcgcttt ctatgctgcc gaacgcccca gcatggttgt caatttccgg
      361 gattattgtc gcagtagttg cgatgtcgaa atcattcctg ggtacgtact ttggcgttat
      421 tgaaggtgcc acagaggtcg tcaaaacaac actacagcag gttggtgtaa agaaaagtcg
      481 tgcatttaac cacgcactat caattatgtt ggtatcgctg attaccttca ttgtttgttg
      541 cattaacccg aacgcgattt cgatgattta cgcgatcagc ggcccgctca ttgccatgat
      601 acttttcatc atgcctacgc tgtcaacgta tctcatcccg gcgcttaaac cctggcgttc
      661 catcggaaat ctgattacgc tgatcgtggg tatcctgtgc gtatcggtaa tgttctttag
      721 ctaatatccg ttgtggatgg ttcaaaattg ctacaatttt gaaccattcc atttttcata
      781 atattcattt gttattattc cgccctatca cttctctcgt catatcctag aattatcagt
      841 tagttaaaca cacaaagaga tctgaacatc ttaaataaga ctatttaaga tgcataactt
      901 agattcgcaa gatatcttct ggtcattcaa aaacaatttg cgatatagat attctcatac
      961 catgagatag ttatctggac cacgttcgac catgcaagat taaatcagtg aaacataatc
     1021 atatttaata acgagaaatg catttttaaa tatcttaatc agaattatag caaatacaga
     1081 ttaatcgca
//
LOCUS       DADMEQ010000295         1078 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.297, whole genome shotgun sequence.
ACCESSION   DADMEQ010000295 DADMEQ010000000
VERSION     DADMEQ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.297"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..276
                     /locus_tag="JNZ05_004181"
     CDS             <1..276
                     /locus_tag="JNZ05_004181"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY8567130.1"
                     /translation="KKPRKQHSPEFRSEALKLAERIGVTAAARELSLYESQLYNWRSK
                     QQNQQTSSERELEMSTEIARLKRQLAERDEELAILQKAATYFAKRLK"
     gene            273..>1078
                     /locus_tag="JNZ05_004182"
     CDS             273..>1078
                     /locus_tag="JNZ05_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HAY8567131.1"
                     /translation="MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQ
                     FRQHCDSVVLAAFTRSKQRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKF
                     SPVSYRAHGLPVSENLLEQDFYASGPNQKWPGDITYLRTDEGWLYLAVVIDLWSRAVI
                     GWSMSPRMTAQLACDALQMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSM
                     SAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATVFNYIECDYNRWR"
BASE COUNT          267 a          280 c          306 g          225 t
ORIGIN      
        1 aaaaaacccc gtaaacagca ttcgcctgaa tttcgcagtg aagccctgaa gcttgctgaa
       61 cgcatcggtg ttactgccgc agcccgtgaa ctcagcctgt atgaatcaca gctctacaac
      121 tggcgcagta aacagcaaaa tcagcagacg tcttctgaac gtgaactgga gatgtctacc
      181 gagattgcac gtctcaaacg ccagctggca gaacgggatg aagagctggc tatcctccaa
      241 aaggccgcga catacttcgc gaagcgcctg aaatgaagta tgtctttatt gaaaaacatc
      301 aggctgagtt cagcatcaaa gcaatgtgcc gcgtgctccg ggtggcccgc agcggctggt
      361 atacgtggtg tcagcggcgg acaaggataa gcacgcgtca gcagttccgc caacactgcg
      421 acagcgttgt cctcgcggct tttacccggt caaaacagcg ttacggtgcc ccacgcctga
      481 cggatgaact gcgtgctcag ggttacccct ttaacgtaaa aaccgtggcg gcaagcctgc
      541 gccgtcaggg actgagggca aaggcctccc ggaagttcag cccggtcagc taccgcgcac
      601 acggcctgcc tgtgtcagaa aatctgttgg agcaggattt ttacgccagt ggcccgaacc
      661 agaagtggcc aggagacatc acgtacttac gtacagatga aggctggctg tatctggcag
      721 tggtcattga cctgtggtca cgtgccgtta ttggctggtc aatgtcgcca cgcatgacgg
      781 cgcaactggc ctgcgatgcc ctgcagatgg cgctgtggcg gcgtaagagg ccccggaacg
      841 ttatcgttca cacggaccgt ggaggccagt actgttcagc agattatcag gcgcaactga
      901 aacggcataa tctgcgtgga agtatgagcg caaaaggttg ctgctacgat aatgcctgcg
      961 tggaaagctt ctttcattcg ctgaaagtgg aatgtatcca tggagaacac tttatcagcc
     1021 gggaaataat gcgggcaacg gtgtttaatt atatcgaatg tgattacaat cggtggcg
//
LOCUS       DADMEQ010000296         1060 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.298, whole genome shotgun sequence.
ACCESSION   DADMEQ010000296 DADMEQ010000000
VERSION     DADMEQ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.298"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..631)
                     /locus_tag="JNZ05_004183"
     CDS             complement(<1..631)
                     /locus_tag="JNZ05_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY8567132.1"
                     /translation="MSKFTVDSFIVELGFNENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLGQLERKFNKTFDKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQ
                     PLISGNRAITAAYSANMSKLKGFDPILQKYIKSQFYGLSAKAGSMDSSKFNEKLAQLN
                     ASVREAIAKARGHTSTSINADRAANSLDNLSSAAVKTAGAFISMQAVLAS"
     gene            complement(840..1043)
                     /locus_tag="JNZ05_004184"
     CDS             complement(840..1043)
                     /locus_tag="JNZ05_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567133.1"
                     /translation="MIDLTERYELIKAISERLKEQPNKPKRIKSSTSKQKNRIRRIAK
                     WCLDKLPSDCQIVGEDGNYYLVR"
BASE COUNT          278 a          220 c          185 g          377 t
ORIGIN      
        1 aactggctaa tacagcctgc attgagataa atgcacctgc tgttttaact gctgctgaag
       61 acaggttatc aagactatta gctgctctgt cagcattaat gctggtggat gtatgacctc
      121 tggctttagc aattgcttct ctgactgaag cgtttaactg agctaacttc tcattgaatt
      181 ttgaagaatc cattgacccg gctttagcag atagcccgta aaactgagat ttgatgtatt
      241 tctgcaaaat agggtcaaag ccttttagct tagacatatt agctgagtaa gccgctgtga
      301 ttgctctgtt gccgctaatt agtggctgtc ttggctgtct aagtggcttt aaagtatcct
      361 caatttccgt tttaatctta aacgctctag tgtttttaag acgctgctca atcttgtcaa
      421 aggtcttatt aaacttcctt tcaagttgcc ctaaagaact tgtcagattg ctgtcaagtt
      481 gcttggtatc gaccttaaac gctctattca aattgcgttc aattctttgt gctgactgca
      541 aagctgcctt ttcaactctt tgcagtccct taataacgtt ttcattgaag cctagctcaa
      601 cgataaaact atcaactgtg aatttgctca ttcatatttt tcacctcttt aagcgtgttg
      661 tacgtaaatt ctatttttca gtgtgggtat tagctttaca gatttcatga ggataattct
      721 tatccgaatc atcacgtagc acacaggatt cgatagaaga ctatctcata tacaaaaaag
      781 ggagagctac cgatgtcact cgataatctc tcccgttgat ttttacccaa ccaatctttt
      841 tacctaacca gataataatt accatcttca ccgacaatct gacaatcact tggtaattta
      901 tcaagacacc atttagcgat tcttctgatt ctattctttt gcttagaggt ggatgatttg
      961 attcgttttg gtttgttggg ttgttctttc aatcgttcac taattgcttt aataagctca
     1021 tagcgttctg ttaaatcaat cattcaccac ctccttttaa
//
LOCUS       DADMEQ010000297         1038 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.299, whole genome shotgun sequence.
ACCESSION   DADMEQ010000297 DADMEQ010000000
VERSION     DADMEQ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.299"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..767
                     /locus_tag="JNZ05_004185"
     CDS             <1..767
                     /locus_tag="JNZ05_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY8567134.1"
                     /translation="FATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRHQ
                     QTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNRRLSESVPCMPHKKAG"
     gene            788..>1038
                     /locus_tag="JNZ05_004186"
     CDS             788..>1038
                     /locus_tag="JNZ05_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY8567135.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     CWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQ"
BASE COUNT          337 a          228 c          235 g          238 t
ORIGIN      
        1 aatttgcaac agataatggt tatacatcag gaaatccatt taacggtctg acaccattaa
       61 aaaagtccaa gatagaacca gatcccctca cccgtgacga atttattcgt tttattgagg
      121 cttgccgtca tcaacaaaca aaaaacctgt ggattatcgc tgtatacacg ggtattcgtc
      181 acggggagct ggtatcactg gcctgggaag atatagacct taaagcgagg actataacca
      241 tccgccggaa ttatacaaaa ctaggcgaat tcactccacc aaaaactgat gctggtaccg
      301 gaagaacgat tcatctagtt caaccagcta ttgatgctct taaaagccaa gcggaaatga
      361 ccatgcttgg aaaacagcat tctgtagagg taaagcagag ggaatatggg agaagtactg
      421 tgcataaatg cacttttgtt tttagtcctc aggtaataaa acagcggcag ttttccggac
      481 cgcactataa ggttgactcc atcagggagt catggacaag tatcttaaaa cgcgcaggtc
      541 tgagacacag aaaatcgtat caatccaggc atacctatgc atgctggtca cttgccgcag
      601 gagctaatcc tagttttatc gcaagccaga tgggccacac aaacgcacaa atggtattca
      661 atgtttacgg agcatggatg aaagacaaca atcacgaaca gatagaactt cttaacagaa
      721 gactatctga aagtgtccca tgcatgcccc ataagaaagc ggggtaaaat aaaaacttgc
      781 aaaatcaatt ggtttacctt taatccctgt cacgttacgc gcgtggcaga ggcgttacgg
      841 attgctgaaa ccgcaacgga cagacggcgg tcatcggctg ttcaacgatg ccgatattga
      901 ccggatccgc gagatcaaat gctggatcga caacggcgtg caggtcagca aagttaaaat
      961 actgctcagt aatgaaaatg ttgatgtgca gaacgtctgg cgcgatcagc aagaaacatt
     1021 actgacttac ctgcaaag
//
LOCUS       DADMEQ010000298         1025 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.300, whole genome shotgun sequence.
ACCESSION   DADMEQ010000298 DADMEQ010000000
VERSION     DADMEQ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.300"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..>1025)
                     /locus_tag="JNZ05_004187"
     CDS             complement(<1..>1025)
                     /locus_tag="JNZ05_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY8567136.1"
                     /translation="EKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSWVFIFAL
                     YFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLAQKPQAI
                     LIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVAGIAMFV
                     MSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGAEKISPY
                     VNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQYYAFQKL
                     GEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCRTNASGT
                     PVNGYFL"
BASE COUNT          303 a          268 c          219 g          235 t
ORIGIN      
        1 tcagaaaata gccattaacg ggcgtaccag aagcgttggt acgacataaa ctggctggaa
       61 tatatttgct gtcagcatca cctaatagca ctttaagcgg ggcatcaatc gagaatacca
      121 gcgcagctac ttgtcccagg gtattcgcaa tggcgtaaat caccattaaa gtattaccca
      181 tgttgtaata ctcgcccagc ttctgaaagg cgtaatattg accgttcgtc attaagtcat
      241 ccgggatatt acgcgagtca aacatcatcc ccatcgccag tgagcccaga atggcacaaa
      301 ccgcaaccat caccgccagg catagcatcc cttttggaaa ttcttttcct gggttgcgcg
      361 tttgattaac gtaaggagaa atcttctctg ctccgccaac cgcgaaaacc agcattgaaa
      421 tagtggtaat gtaggtaaag tcgatatgag gaatgaacgt ttcccaggta atgtttgtgg
      481 tcgcaatatg cacttcagta atcgcaggcg cggttaccgc catcgccaca tacaggagcg
      541 acataacaaa catcgcaatc cctgccacag aaccgacgat ttttagcgat ttcataccgc
      601 gtgaagcaac ccacataaag aagataaaca gcaccagcgt taacccctgt aacgctacga
      661 ctgaatattc tttgattagc gaaccgtcgc ctttcatcgc ccaaccgagc gcaatcagaa
      721 ttgcctgggg tttttgtgcc agatagggaa tatgcaccac ccagtaggtc cacgcggcga
      781 gataagccag tccgggtccc atcgtgtgtt taatccaggt actgaccccg cccttcccat
      841 ctttgaaggt cgagcctaac tgaccaacaa ttagcgcata aggtgtgaaa tagagtgcaa
      901 agataaacac ccatgaaaaa acaaccacca gcccctggtt ggcatagtta ttaacaacgt
      961 tgccaaaacc ccagacagtg ataaaagaca tcagcgcaat gttgtaccat cgcaactgtt
     1021 tttcc
//
LOCUS       DADMEQ010000299         1017 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.301, whole genome shotgun sequence.
ACCESSION   DADMEQ010000299 DADMEQ010000000
VERSION     DADMEQ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.301"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..273
                     /locus_tag="JNZ05_004188"
     CDS             <1..273
                     /locus_tag="JNZ05_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY8567137.1"
                     /translation="IRRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSH
                     ADTQFKNVPSAFQVGYINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            275..>1017
                     /locus_tag="JNZ05_004189"
     CDS             275..>1017
                     /locus_tag="JNZ05_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567138.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEEGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIAPEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTTV"
BASE COUNT          323 a          186 c          201 g          307 t
ORIGIN      
        1 atccgtagaa atgatggttc gatccgcttt aacaataaga tgccttatca cgttacgctg
       61 ggttatatcg gaacaaacgg aatcacaatg ttgccacaaa cgcaaagttt aatggtaacc
      121 ccttatagcc atgcagatac gcagtttaaa aatgttccgt cagcttttca ggttggttat
      181 atcaatgatt ttggtggatt aagtttttat gaaataaact gcccgacggt taataactct
      241 tgtaatgttt ccgtagccaa acgagataaa taatatgcgc tacttattaa ttgtaattat
      301 atttattata ggttttagtg cattacctgt atgggcaatg gactgctatg ctgaacatga
      361 agagggaaat accgtagtca ttggttacgt accaagaatt tctatcccca gtaatggtaa
      421 aaaaggtgat aagatctggc aaagcagtga gtattttatg aatgtcttct gtaataatgc
      481 attacctgcc ccatcacctg gagaagaata cccatcagcc tggactaata taatgatgtt
      541 tttaccaggc ggtcaggact tttataatca gaactcctat atttttggcg taacatacaa
      601 tggagttgat tatgattcga ctgcccctaa tgctatcgca gcaccagaat gcattgatat
      661 aaaaggagcg ggtaccttta ataatcacta taaaaacccc gctgtctgta gtggaggtcc
      721 tgagcctcaa ttatcagtaa cctttccagc tcgcgtacaa ctctatatta aattagcaaa
      781 aaatgccaat agagttaata aaaacctggt gttacctgat gaatatatcg ctccagagtt
      841 taaaggtatg agtggcgcag gagctataga agtagacaaa aatttgacct tcaggattcg
      901 tggattaaat aatattcatg tccttgactg ctttgttaat gtcgcactgg aaccagctga
      961 tggcgttgtt gactttggta aaataaattc ccgaacaatt aaaaatacca ccgttag
//
LOCUS       DADMEQ010000300          999 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.302, whole genome shotgun sequence.
ACCESSION   DADMEQ010000300 DADMEQ010000000
VERSION     DADMEQ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.302"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(1..186)
                     /locus_tag="JNZ05_004190"
                     /pseudo
     CDS             complement(1..186)
                     /locus_tag="JNZ05_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(137..999)
                     /locus_tag="JNZ05_004191"
                     /pseudo
     CDS             complement(137..999)
                     /locus_tag="JNZ05_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          223 a          265 c          257 g          254 t
ORIGIN      
        1 gtcggtgcga catcatcagc ggtggctgtg taggggactg cctggtccct gtcgtcaaaa
       61 aaaaacgtca cagtctactg cgccactttc ggtataacgg ggattaatga tttttttaat
      121 ttccacggtg cattcctcac gatgtgcgaa taaaaagccc gggcattgcg ccagagacat
      181 gtgcatccgg caccccggac agggcgcaat atgaccccgg taatgaatgc tctgaacatc
      241 ccgtaatgaa aaattgtggg gatactatat acgttccggt gggagtactg ggcactgaaa
      301 tccccaccgg tcccagtcgt gagcctctgt atgactgccc cctgacaagt ctgatgactt
      361 tatcaccgtc agcttctccc tggtacgcag caataatcag cccgccaatg tcagggtctc
      421 cccatctgtt gcggacagag ctcgccacga ttctgtaaat aatatcttct gtggttatat
      481 ttattttcac ccagtcagtc agtctggata tgggccagta gcagtagcgg gtgtgataaa
      541 tggggccgtt aatgccgtaa aaagtaaggg atttggctct gtaccgcggt tctgttgtct
      601 cagggcgtgc atcagtccac cggatgctga gcaccccttc aaaccgtgtg tcgggtatga
      661 tgatgccgta agggccagca acggaatatt cacctggcag cgcattcctt acctaggcca
      721 ggaaatcact cttagtgtca aaacggataa catcttcagg cagaaaagca cacccaaagc
      781 cgaatgcgcc gggtatcgcc agacggcctt ttgtccggtc gtaaatgtcg ctctgtgctt
      841 ccatcgtggc cgcacttttc agccccagat tatcccggga cttctgttgt gccttttcgc
      901 ctgctgctgc gatttcagac agatggttag ccgttttcag agtgccggtc agcgcagcat
      961 caatgtcatt tttggcctgc tctgctgcgc gggcataac
//
LOCUS       DADMEQ010000301          996 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.303, whole genome shotgun sequence.
ACCESSION   DADMEQ010000301 DADMEQ010000000
VERSION     DADMEQ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.303"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(35..325)
                     /locus_tag="JNZ05_004192"
     CDS             complement(35..325)
                     /locus_tag="JNZ05_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HAY8567139.1"
                     /translation="MNAHWSSKKSNFLRKNIKLLTKYLFFESQGIPDKVDIVSRLKTY
                     GYSISGVETDDGYKALVRAFQLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
     gene            complement(294..545)
                     /locus_tag="JNZ05_004193"
     CDS             complement(294..545)
                     /locus_tag="JNZ05_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY8567140.1"
                     /translation="MEPVYVILNALLDSGRFTRKLILLGLSGTFSYIFGSIVATLGMG
                     LVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLEFEKE"
BASE COUNT          276 a          262 c          219 g          239 t
ORIGIN      
        1 aaggggatat atcgcagctc tgacgactca ctccttattt acccgggaaa tacttctcca
       61 gcaatgcata caggatggca gcggtttcag catccattat gccgtcataa tttttctgcc
      121 ggaaatgaag ctgaaaagcg cggacaagcg ccttatatcc gtcatccgtc tccacaccag
      181 aaatactgta tccataggtc ttcagccgtg acacgatatc aaccttatcc ggaatgccct
      241 gcgattcaaa aaataaatat ttagtcagta attttatgtt tttcctgaga aaattactct
      301 ttttcgaact ccagtgagcg ttcacgactc atcagtgtga aaatgatggc aaaaacagca
      361 gacagtatca gtacgataaa tgtggccccc catcccagat aatcgacaac aagtcccatt
      421 cccagtgtcg caacaattga gccgaagata taggaaaatg tgcctgacag ccctaacaga
      481 ataagtttac gcgtaaatcg cccggaatcc agaagagcat taagtatcac ataaacaggt
      541 tccataccag tcctgaccgc atcaggacgc cagtaaataa aatctcgtag tccaccggca
      601 tcagagacaa gagtaatcgt cgttaccggt atttcgactg gtttgtactg gtgtgagcgg
      661 acaaaactac tgtacgtctc tcatacacgc tcgtaagcgg ccctcacggc ccgctaacgc
      721 ggagatacgc cccgactgcg ggtaaaccct tgtcgggacc actccgaccg cgcacagagg
      781 cattttcatc gctgacaggc cgcatggcct cgcagagccg gagggggact ggtgatattc
      841 ggacgaccgt accggcttgc gccgggcttc ggcggtttta cacctgtgct acatgtaacc
      901 accgtatgcc gccttatatg cgctgactcg cattatttgc cttgttccgt ctgaaagtaa
      961 tcactatgat taaatatgat taacaactaa tcgggt
//
LOCUS       DADMEQ010000302          988 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.304, whole genome shotgun sequence.
ACCESSION   DADMEQ010000302 DADMEQ010000000
VERSION     DADMEQ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.304"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            13..486
                     /locus_tag="JNZ05_004194"
     CDS             13..486
                     /locus_tag="JNZ05_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8567141.1"
                     /translation="MAIAFKNIQAGTARKFTLSSANNSTKNAAFYLWGNPSRPVVAEL
                     GDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGHEMYYSP
                     GSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNVASI"
     gene            546..>988
                     /locus_tag="JNZ05_004195"
     CDS             546..>988
                     /locus_tag="JNZ05_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY8567142.1"
                     /translation="MTKRYSVTWLFSEDGKNWYEELKNFASDTIKIAYTGDGRVVWVG
                     KDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVS
                     ARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFSTITDKSS"
BASE COUNT          286 a          209 c          257 g          236 t
ORIGIN      
        1 tttcagaatg gagtggctat tgcttttaag aatattcagg ccggaactgc cagaaaattc
       61 acgttatcca gcgccaacaa ctccacgaaa aatgcagcgt tttatttgtg gggtaatcca
      121 tccaggcctg ttgttgcaga gcttggtgat gattcaggct ggcatttttt cagccagaga
      181 aacccggata acagcatagt gttcactgtt aacggacagg taattccgtt aaattacgga
      241 aacttcgatg cccgctataa atatcgaaca gagggggtac aggatgtacg gtatggccat
      301 gaaatgtatt acagccccgg cagtaacacc gtttcgtgga gattttgcgc accttcggga
      361 cacgggctgt cagggatggc gatatcggat accggccgta actcagcgga taacgttgac
      421 ggtgtgtatt accgaccact gcaaaaactg attaatggca cctggtataa cgtagcgagt
      481 atttaacaat gttgcattta aaaaatatta ctgcgggtaa tccgaaaaca gcagaacaat
      541 atcagatgac aaaacgatat tcggtcacct ggcttttttc agaagacgga aaaaactggt
      601 atgaagagct gaagaatttc gccagcgaca caataaaaat agcttacacc ggagatggtc
      661 gcgtggtgtg ggtcggtaag gatgtgacag gcatcgagcc acgcaatgcc agcgttatcg
      721 aagttccgga tattaccgct aaccgacgga ttaccgcgcc gggttactgg ttttaccgca
      781 atgatgaatt tgtctttgac tacagactca aagcggaaga tgagcgtgat gcccttctgg
      841 ctcaggtcag tgcccggaca ggggaatggg aagaagacct gctgctgggg ttaatcagcg
      901 acgaagataa agaaaagctg aaagcctgtc gtatttacgc gaaatcgctg caggcgatgg
      961 atttcagcac catcactgat aaatcctc
//
LOCUS       DADMEQ010000303          983 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.305, whole genome shotgun sequence.
ACCESSION   DADMEQ010000303 DADMEQ010000000
VERSION     DADMEQ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.305"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JNZ05_004196"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JNZ05_004196"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HAY8567143.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMKALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDHLVSPEGLAPEWD"
BASE COUNT          259 a          219 c          221 g          284 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggt
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctt cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DADMEQ010000304          977 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.306, whole genome shotgun sequence.
ACCESSION   DADMEQ010000304 DADMEQ010000000
VERSION     DADMEQ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.306"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            87..977
                     /locus_tag="JNZ05_004197"
                     /pseudo
     CDS             87..977
                     /locus_tag="JNZ05_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007946.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
BASE COUNT          258 a          229 c          242 g          248 t
ORIGIN      
        1 gttgtttact gtgcgctgtt gtccatgtga tattgtgtac cagatgtgtg ccaaactatt
       61 atttctgagc gtaccaaata ttatttatgg ctataagcga cacaaaactg cgtactattt
      121 atggtaaacc atattcgggc ccacaagaag tggctgatgc cgatggcctc agcgtacgaa
      181 tttcaccgaa gggggtcatc cagttccagt accgctatcg ctggcatggc aagcctaatc
      241 gacttgggct tggtcgatac ccatccctgt ctttgaagga tgccagacag atcactgctg
      301 acttgcgaaa gctctatttc tcaggaacgg atccacgcac ctattttgaa gagaaggtgg
      361 agaactccat gacggtcgcc cagtgtctcg actactggtt cgacaactac gtctctacaa
      421 ctctcagaga aaaggcctag gcactttatc gatcaacggt tatgaagcgc atgcatgacg
      481 ccttccctaa tcgtccggca tcttctatca cggttaagca atgggttgac ctgcttaccg
      541 aagaagaaaa agataatcca cgccgagcaa ggcaggtgct aagtcaacta agatcagcaa
      601 ttagttggtg catgcggcgt cagttgatag atagttgcgc aattatgagc atccagccaa
      661 gggacttcgg ctcccgcgct gaggtagggg atcgggtact gtcgtatcac gaactggcta
      721 agatttggct tgctattgaa agaagccgtg cgtctacgtc aaataagctc cttcatcaga
      781 tgcttatgct gtggggggcg aggctctcag agcttaggct ggcaacaaag acagaatttg
      841 acctgctgga caacgtatgg accgtaccga aagagcatag caagatgggt aatgttatcc
      901 gccgtccaat cttcgaacaa attaagcctt ttctcgaaaa ggccatgaca atgtacaatg
      961 atgttctttt ccctgga
//
LOCUS       DADMEQ010000305          968 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.307, whole genome shotgun sequence.
ACCESSION   DADMEQ010000305 DADMEQ010000000
VERSION     DADMEQ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.307"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..>968
                     /locus_tag="JNZ05_004198"
     CDS             <1..>968
                     /locus_tag="JNZ05_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013141603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAY8567144.1"
                     /translation="YIARLRNRVENRLWHSLAACIDDSQTQQLLDLLSVPAGSRYSLL
                     DQLRAGPTKVNATSLVQAIGRLQTIRSLGVTLPAITPVSDIRIAAMARYASTAKITAL
                     QRLPEKRKLATLVAFSCCMEATAQDDALELLEALLRDLFNEAVQADKRNRQRTLKDLD
                     RAAEILAKACRMLLDDKLSDTDVRDSIFNIIPEDVLTHAVNNVTSIIRPDNNVYFNEL
                     DSKFKTVRRFLPDLLSRIHFEGNASAKTLIEALCWIEVNLKKKKTDNDAPREIINKPW
                     QQHVIRKDGSIDFHAYTFCALKELQLALKKRDIYVNPSWRYADPRA"
BASE COUNT          289 a          216 c          204 g          259 t
ORIGIN      
        1 ctatatagcc cgattacgca acagagttga aaaccgtctc tggcattcac tggcagcttg
       61 cattgatgac tctcagactc aacagcttct ggatcttctt tcggttcctg ccggaagccg
      121 ctattcgtta cttgatcaat tacgcgccgg acctactaaa gtgaatgcca catcactggt
      181 acaggctata ggtcgccttc agacgatacg tagcctagga gtgacattac ctgcaattac
      241 gccagtttcg gatatccgta ttgcggccat ggcacgttat gcctcaacgg ctaagataac
      301 agcgcttcag cgcttaccag aaaaaagaaa actggctacg cttgttgcat tctcttgctg
      361 catggaggcg acagctcagg atgatgctct tgagttactt gaagcgctac tccgggatct
      421 gtttaatgaa gcggttcaag cagataaacg taaccgccaa cgaacactta aagatctgga
      481 tcgtgcagca gaaatactgg caaaagcatg tcggatgctt cttgacgata aattatctga
      541 tactgatgtt cgtgatagta tcttcaatat cattcccgaa gatgtactga ctcatgcagt
      601 aaataatgtg acatcaatta ttcgaccaga taataacgtt tacttcaatg aacttgattc
      661 taaattcaaa acggtgcggc gtttcttacc tgacttactt tccagaatac attttgaggg
      721 caatgcctca gccaaaacat taattgaagc gctttgctgg attgaagtta atttaaaaaa
      781 gaaaaaaaca gataacgatg caccacgcga aataatcaat aaaccgtggc aacagcatgt
      841 gataagaaaa gatggtagta ttgatttcca cgcctataca ttttgtgcac ttaaggaact
      901 tcagcttgca ctgaaaaaac gggatattta tgtcaatccc agctggcgtt atgcagatcc
      961 ccgggcag
//
LOCUS       DADMEQ010000306          955 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.308, whole genome shotgun sequence.
ACCESSION   DADMEQ010000306 DADMEQ010000000
VERSION     DADMEQ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.308"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            1..948
                     /locus_tag="JNZ05_004199"
                     /pseudo
     CDS             1..948
                     /locus_tag="JNZ05_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          249 a          235 c          274 g          197 t
ORIGIN      
        1 acaacgcatt gaaatggaga atgaaatatt aaaaaaggct accgcgctct tgatgtcaga
       61 ctccctgaac agttctcgat aatcgggaaa ctcagagcgc attatcctgt ggtcacactc
      121 tgccatgtgt tcggggttca tcgcagcagc tacagatact ggaaaaaccg tcctgaaaaa
      181 ccagacggca gacgggctgt attacgcagt caggtacttg agctacatgg catcagccac
      241 ggttcggccg gagcaagaag catcgccaca atggcaaccc ggagaggcta ccagatggga
      301 cgctggcttg ctggcaggct catgaaagag ctggggctgg tcagctgtca gcagccgact
      361 caccggtata aacgtggtgg tcatgaacat gttgctatcc ctaactacct tgaaaggcag
      421 ttcgccgtga ccgagccaaa tcaggtgtgg tgcggtgatg tgacctatat ctggacgggt
      481 aagcgctggg cgtacctcgc cgttgttctc gacctgttcg caagaaaacc agtgggctgg
      541 gccatgtcgt tctcgccgga cagcaggctc accatgaaag cgctggaaat ggcatgggaa
      601 acccgtggta agcccggcgg ggtgatgttc cacagcgatc agggcagtca ttatacgagc
      661 aggcagttcc ggcagttatt gtggcgatac cagatcagac agagtatgag ccggcgcgga
      721 aactgctggg ataacagccc aatggaacgc ttcttcagga gtctgaagaa cgaatggatg
      781 ccggtggtgg gttacgtaag cttcagcgag gcagctcacg ccataacgga ctatatcgtt
      841 ggatattaca gcgcactaag accgcacgaa tataacggtg ggttaccccc aaacgaatcg
      901 gaaaatcgat actggaaaaa ctctaactcg gtggccagtt tttgttgacc acttc
//
LOCUS       DADMEQ010000307          922 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.309, whole genome shotgun sequence.
ACCESSION   DADMEQ010000307 DADMEQ010000000
VERSION     DADMEQ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.309"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..>922
                     /locus_tag="JNZ05_004200"
     CDS             <1..>922
                     /locus_tag="JNZ05_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HAY8567145.1"
                     /translation="IELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQLDRLRRMLF
                     GQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSPPSENPIAS
                     KPESPGRKSSRKPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQLDIINTAFK
                     VIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIPLYRQSEIY
                     ARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTK
                     TGRLWVYVRDDRNAGSSLPAAVWFAYSADRK"
BASE COUNT          252 a          233 c          248 g          189 t
ORIGIN      
        1 attgagctgc ttaaagccat gttgcgtaag caacagagtc ggcttcgaca gtatgcctgt
       61 caggtcgcgg gctatgagca ggaaattgaa cggctgaaag cgcaactcga caggttgcgt
      121 cgtatgttgt tcggccagag ttcagagaaa aagcgtcata agcttgaaaa tcagatccga
      181 caggcagaaa aacgactgtc ggaactggaa aaccggctga acacagccag aaatcttctg
      241 gaagatgcat cgtcagtcac agattcacct gacaccagtc ccccgtcaga aaacccgatc
      301 gccagtaagc ctgaatcccc gggacgaaaa tcttcacgaa aaccgctgcc ggcagaactt
      361 ccccgggaga cacatcgcct tctgcctgct gaaaccagtt gcccggcctg tggaggtgtt
      421 ctgaaagaaa tgggggaaac aatctcagag caactggata tcattaatac cgcctttaaa
      481 gttatcgaaa ccatacgtcc caaactggcc tgtagccggt gtgatgtcat cgttcaggca
      541 ccacttcccc ctaaaccgat cgaacgcggt tatgccagtg cagggttact tgcacggatc
      601 ctggtcagca aatatatgga acatatccct ttatatcgcc agtcagaaat atacgcgcga
      661 cagggcgtgg agctgagccg taataccatg gtgcgctggg tatcagaaat ggcagacaaa
      721 ctccgtcctc tgtatatagc gctgaatgac tatgttctgg aggcaggaaa ggtgcacgca
      781 gatgacactc cggtgaaagt actggccccg gggaacggaa agacgaaaac gggtcgtctg
      841 tgggtatacg tcagggatga tcgtaatgcg ggttcatccc tgccggcagc cgtctggttc
      901 gcgtattcgg cagatcgcaa ag
//
LOCUS       DADMEQ010000308          894 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.310, whole genome shotgun sequence.
ACCESSION   DADMEQ010000308 DADMEQ010000000
VERSION     DADMEQ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.310"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(110..439)
                     /locus_tag="JNZ05_004201"
     CDS             complement(110..439)
                     /locus_tag="JNZ05_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HAY8567146.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(611..>894)
                     /locus_tag="JNZ05_004202"
     CDS             complement(611..>894)
                     /locus_tag="JNZ05_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567147.1"
                     /translation="FVLLNAQKLRDTQHMMQQILLSLVHALYEGNPQPVFANTEKLND
                     AVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          218 a          207 c          198 g          271 t
ORIGIN      
        1 tgttgttaat ttcgattggt tctcagatga gaatgatgac ggtgatacac agataagaat
       61 ctatgagcaa tgagggttgc cgggcaaccc tcattgaata aaacgggaat tacttcgctt
      121 cgccgttttt catttcgccc atagctttca gctttttgga gatatcgcgg cgttctttgg
      181 agagctcggc atttttgatg atgtaatcgt caacgcgatc ttcatagtca cctttcatgc
      241 tggcgatgat gccctggatt gcttcaacgc tcatccctgg cttgatgtaa tcgctcaggt
      301 tgtccagcaa caggacgcgc ttctggttgt cacggatctt tttctcaacg tcctgaattt
      361 cacgttgcag tttgttctta cgacggaaca gacgaacaaa ttccagtacg tcctggaatg
      421 aaggcttggt agtttccatt tttatacccc tgataatgtg agagtcggat tcgtttaagc
      481 aaccgctatt cgttagggcc aacattactc atagctgccg cagatgacaa tgcttttatc
      541 ccttttcact atcataccct ttatccttgc tgaatcgaag cagcagcaag atgattctga
      601 agttcaggaa ttatttgcgc aaggcccggc aaatcagatt cgatagcaac tcaagctgat
      661 gtgccgtttc catgttcagc cacacataac catagattgg tgtttccacg actttcaagt
      721 catggtggtt atcgagcaac tgacgcagct cttccacagc atcgttcaat ttttccgtat
      781 tggcaaaaac cggctgcgga ttaccttcgt acagcgcatg aacaaggctc agcagtattt
      841 gctgcatcat gtgctgggta tcacgaagtt tttgcgcgtt caataacacg aaat
//
LOCUS       DADMEQ010000309          881 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.311, whole genome shotgun sequence.
ACCESSION   DADMEQ010000309 DADMEQ010000000
VERSION     DADMEQ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.311"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..433
                     /gene="ospC3"
                     /locus_tag="JNZ05_004203"
     CDS             <1..433
                     /gene="ospC3"
                     /locus_tag="JNZ05_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC3"
                     /protein_id="HAY8567148.1"
                     /translation="KLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKADI
                     ALYLLSKFNFTKQDVAEMEKMNNNRYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVN
                     KKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
     gene            771..>881
                     /locus_tag="JNZ05_004204"
     CDS             771..>881
                     /locus_tag="JNZ05_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8567149.1"
                     /translation="MSRKNQRYSTEFKAEAVKTVPENQLSISEGASRLSVP"
BASE COUNT          298 a          155 c          183 g          245 t
ORIGIN      
        1 aaaactcaga gacatatctt tggaggatgc gattaaagca tctaattatg aagagattaa
       61 caataaagtg actgataaaa aaatggctca tcaggccctt gcttattctt taggcaataa
      121 aaaagctgat atagcacttt atttactatc taaatttaat tttacaaaac aagatgtcgc
      181 agagatggag aaaatgaaca acaatagata ttgtaatctg tatgatgttg aatacttact
      241 tagtaaagat ggtgccaact ataaagtgct agaatatttt atcaataatg gattggttga
      301 tgtaaacaaa aaatttcaaa aagcaaatag tggggacact atgcttgata atgcaatgaa
      361 aagcaaagat tcaaaaatga ttgatttttt attaaaaaat ggagcaatat taggcaaacg
      421 atttgagata tgacctaaaa gctcggccaa ataataatgt cgtcgattat cgaagtcatc
      481 atgagtgatt ctgtccgcac agtgcagcat cagcatgaca gtgaattgtc tggcgtggtc
      541 catttaatga gatggagatg gtccctcttt cctaaatagc tgtgcctaat accgctacac
      601 ttttgccagc ccatgtttgc ctccggggaa tgggctgacg gttccttcaa aatccattgc
      661 acgcagttat gtcggagcgg gaacttgact cctgtcttta cgggagtgag gtagcctgag
      721 tttaacggac actccttcct gaaatagaat ggcatcagaa ggagccaata atgagcagaa
      781 aaaaccaacg ttactctacg gagtttaaag ccgaagccgt caaaacggtt cctgaaaatc
      841 agctttcgat cagtgaaggc gcttcccgat tatctgttcc t
//
LOCUS       DADMEQ010000310          879 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.312, whole genome shotgun sequence.
ACCESSION   DADMEQ010000310 DADMEQ010000000
VERSION     DADMEQ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.312"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..413
                     /locus_tag="JNZ05_004205"
     rRNA            <1..413
                     /locus_tag="JNZ05_004205"
                     /product="16S ribosomal RNA"
     gene            470..546
                     /locus_tag="JNZ05_004206"
     tRNA            470..546
                     /locus_tag="JNZ05_004206"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:504..506,aa:Ile,seq:gat)
     gene            589..664
                     /locus_tag="JNZ05_004207"
     tRNA            589..664
                     /locus_tag="JNZ05_004207"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Ala,seq:tgc)
BASE COUNT          241 a          194 c          239 g          205 t
ORIGIN      
        1 gggaactcaa aggagactgc cagtgataaa ctggaggaag gtggggatga cgtcaagtca
       61 tcatggccct tacgaccagg gctacacacg tgctacaatg gcgcatacaa agagaagcga
      121 cctcgcgaga gcaagcggac ctcataaagt gcgtcgtagt ccggattgga gtctgcaact
      181 cgactccatg aagtcggaat cgctagtaat cgtggatcag aatgccacgg tgaatacgtt
      241 cccgggcctt gtacacaccg cccgtcacac catgggagtg ggttgcaaaa gaagtaggta
      301 gcttaacctt cgggagggcg cttaccactt tgtgattcat gactggggtg aagtcgtaac
      361 aaggtaaccg taggggaacc tgcggttgga tcacctcctt accttaaaga agcgttcttt
      421 gtagtgctca cacagattgt ctgatgaaaa tgagcagtaa aacctctaca ggcttgtagc
      481 tcaggtggtt agagcgcacc cctgataagg gtgaggtcgg tggttcaagt ccactcaggc
      541 ctaccaaatt tgcacggcaa atttgaagag gttttaacta catgttatgg ggctatagct
      601 cagctgggag agcgcctgct ttgcacgcag gaggtctgcg gttcgatccc gcatagctcc
      661 accatctctg tagtgattaa ataaaaaata cttcagagtg tacctgcaaa ggttcactgc
      721 gaagttttgc tctttaaaaa tctggatcaa gctgaaaatt gaaacactga acaacgagag
      781 ttgttcgtga gtctctcaaa ttttcgcaac acgatgatga gtcgaaagaa acatcttcgg
      841 gttgtgaggt taagcgacta agcgtacacg gtggatgcc
//
LOCUS       DADMEQ010000311          852 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.313, whole genome shotgun sequence.
ACCESSION   DADMEQ010000311 DADMEQ010000000
VERSION     DADMEQ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.313"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..348
                     /locus_tag="JNZ05_004208"
     CDS             <1..348
                     /locus_tag="JNZ05_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HAY8567150.1"
                     /translation="LTDWHYTVSETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYS
                     HLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEG
                     PFSKDVILKITYN"
     gene            complement(391..591)
                     /gene="glgS"
                     /locus_tag="JNZ05_004209"
     CDS             complement(391..591)
                     /gene="glgS"
                     /locus_tag="JNZ05_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HAY8567151.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRLVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          261 a          186 c          158 g          247 t
ORIGIN      
        1 ttaactgact ggcattacac cgttagtgag acgtttagtg tagtcatgac aaaagatcct
       61 ggtgcggcct gtactgagca attcaatatt ttagggagct ttttcactac ggatattttg
      121 agtgattata gccatctgga tatgggtaat ggactgctat tgaagatatt tcataaagat
      181 ggaacagcaa cggaatttaa ccgcttctca caatttgctt ctttttcatc gtctagtgca
      241 ccttcggtca ccgcaccatt cagggcagaa cttagtgcga acccggcaga gactgttgtt
      301 gagggaccgt ttagtaaaga cgtaatcctc aaaatcacct ataactagca tctaatacaa
      361 acactaaaac gggccatcag gcccgttata tcagtgctct aactccagct ctcttgcctg
      421 catcatctgt tggcaatacc aggcataacg tgcgcaaaac caggttctat gttcttcatc
      481 catgttacca gtaacggcca gaatatcgac caggcgacct tctgcatgca ttctggcaaa
      541 actacgcgcc aggaaatcga aattattcaa agaattaaga ctatgatcca taagcacatc
      601 ctccggttat cgcatacctt ttaaggccat atgctttttc tgatttcagt attccccaat
      661 cagggtagtg cctttaaaca taacgtgcgt aaatatattg tccccgatca cactttttag
      721 tgaaaaataa aaaaccatat taaaatactt tctcttccag aacttctcta acatgtgtta
      781 ttattaacaa agaaaaatgt aagaagggaa attattccaa ggtaaatatt gcgtcactaa
      841 atggacattg ga
//
LOCUS       DADMEQ010000312          851 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.314, whole genome shotgun sequence.
ACCESSION   DADMEQ010000312 DADMEQ010000000
VERSION     DADMEQ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.314"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            1..759
                     /locus_tag="JNZ05_004210"
                     /pseudo
     CDS             1..759
                     /locus_tag="JNZ05_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          230 a          184 c          202 g          235 t
ORIGIN      
        1 cgcattctga ccgaccgtga aaatggtgaa aaaggcgata attctcaaga tacctcttat
       61 gcccgtgtag gcgtgaaagg tgaaactcag atcaaccctg aaatgactgg ttacggtcag
      121 tttgaactag acctggaagc atcaaacaga cacaaccctg accaaactcg tttggcatat
      181 gctggtttga gttacaaaga ttttggttca ttcgactaca gtcgtaatgt cggtgtcgcg
      241 tatgatgcag aagcatttac tgatatgttt gttgagtggg gcggtgactc atgggctggt
      301 actgacctgt tcatgaccaa ccgtaccaac ggtgttgcaa cctatcgtaa cacagatttc
      361 tttggcatgg ttgaaggtct aaattttgct ctgcaatatc agggtaaaaa tgaaggtaca
      421 ggcaactaca aagcaaatgg cgatggccat ggtttatctg caacttacac tattgacggt
      481 ttctcatttg ctggtgctta tgcaaactct gaccgtactg attggcagtc tggtgatggt
      541 aaaggcgaaa gagccgaagt ttgggcgttg tcaacaaaat atgacgctaa caatgtttat
      601 gctgctgtga tgtacggtga atctcacaac atgaattccg acgacggtga tgttgtgaac
      661 aaaacacaaa acttcgaagc tgttctgcaa tatcagttcg actttggtct gcgtccatcc
      721 atcggttact cctattctaa agcactggac gtagcaggtt ggatttgccc ctatatttcc
      781 agacacctgt tatcacttaa cccattactg gcccgctgcc gtagatattc ccgtggcgag
      841 cgataaccca g
//
LOCUS       DADMEQ010000313          809 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.315, whole genome shotgun sequence.
ACCESSION   DADMEQ010000313 DADMEQ010000000
VERSION     DADMEQ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.315"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            101..>809
                     /locus_tag="JNZ05_004211"
     CDS             101..>809
                     /locus_tag="JNZ05_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY8567152.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLMVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQN"
BASE COUNT          281 a          154 c          140 g          234 t
ORIGIN      
        1 caatacctgg agaaagagta caaagcaaac tgtattaaac ttaataagtt atattgtatt
       61 tcactagagt tcttatcgtt gataagaaat tctggttata atgattaaat caacaaatat
      121 acaggtaatc ggttctggta ttatgcatca aataaacaac atacactcgt taactctatt
      181 ttctttgcct gtgagtttga gtccatcttg taatgaatat tatttaaaag tctggagtga
      241 atgggaaaag aacggtaccc caggcgagca acgcaatatc gccttcaata ggctaaaaat
      301 atgtttgcaa aatcaagggg cggaattaaa tctatctgag ttagatttaa aaacattacc
      361 tgatttaccg cctcagataa caacactgga aataagaaaa aacctattaa catatctccc
      421 tgatttccca ccaatgctta aggtaataca tgctcaattt aatcaactgg aaagcttacc
      481 tgccttaccc gagacgttag aagagcttaa tgtgggtgat aacaagataa aagaattacc
      541 atatcttcct gaaactctaa ctcatttacg gatccataat aaccgattgc atattctgcc
      601 actattgcca ccggagctaa aattactgat ggtttctgga aacagattag acagcatccc
      661 cccctttcca gataagcttg aagggctggc tctggctaat aattttatag aacaactacc
      721 ggaattacct tttagtatga acagggctgt gctaatgaat aataatctga caacacttcc
      781 ggaaagtgtc ctgagattag ctcagaatg
//
LOCUS       DADMEQ010000314          791 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.316, whole genome shotgun sequence.
ACCESSION   DADMEQ010000314 DADMEQ010000000
VERSION     DADMEQ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.316"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            72..353
                     /locus_tag="JNZ05_004212"
     CDS             72..353
                     /locus_tag="JNZ05_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY8567153.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            319..>791
                     /locus_tag="JNZ05_004213"
     CDS             319..>791
                     /locus_tag="JNZ05_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY8567154.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VAQAIEANHYIYKNYSPAALIEKLKGVDSFTMADWIERYKTILIRRKASRNTYKTRVN
                     QLVTIKENLGGVLLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEG"
BASE COUNT          277 a          152 c          176 g          186 t
ORIGIN      
        1 ttattaaaaa tcaaccacac ccgccagtat tctgtatatt tactggcggt catatcgtga
       61 gaggtatggc aatgaatctt gtgacactca aaacgtgggg aaaactcaga tatccggata
      121 acccaccatc aatatcaacg ctgagacgat gggcaaggaa tggaaacatt tatcctgcac
      181 ctgaactaca cgggaggagt tacagggtgg ttccggaggc tttctatatc aacccaaata
      241 aggttgatac cgatataaca caccatcagc ctaatgggcg acaagggaga gacagtccgt
      301 taatggagaa gttaaaacat gcagcggaaa aaatacgatc ccaatttgcc tagaaactta
      361 acatatcgaa ggagagacaa agcatattac tggcgcaacc ctctgacgaa agaagaattt
      421 acactaggta aaatttcaag aagagatgca gtagcgcagg caattgaagc aaatcattat
      481 atatacaaaa actactctcc tgctgcccta attgaaaagc ttaaaggggt cgactcattt
      541 actatggcag actggattga acgttacaaa acgattctta taaggagaaa agcgtccaga
      601 aatacttata aaactcgggt aaatcaactg gtgacaataa aagaaaattt aggaggggtt
      661 ttactgacag aaataaccac tcgccatatc gccgagtttc ttgatttgtg gattgaagga
      721 gggaaaaaca cgatggcagg atcaatgcgt tctgtgttat ctgatatgtt ccgcgaagcc
      781 attgttgaag g
//
LOCUS       DADMEQ010000315          790 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.317, whole genome shotgun sequence.
ACCESSION   DADMEQ010000315 DADMEQ010000000
VERSION     DADMEQ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.317"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..171
                     /locus_tag="JNZ05_004214"
     CDS             <1..171
                     /locus_tag="JNZ05_004214"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567155.1"
                     /translation="KKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGGMYCPECG
                     TVHWPDGVAPPF"
     gene            171..>790
                     /locus_tag="JNZ05_004215"
     CDS             171..>790
                     /locus_tag="JNZ05_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8567156.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVSHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMP"
BASE COUNT          166 a          208 c          237 g          179 t
ORIGIN      
        1 aaaaagctgg tcttctggtt tgtacgtgtt gatgacgaag ggtatccgga aatcgcccgc
       61 tgcacggagc gggaatttgc gaccattctt gccggtatca gcgccggcgg tatgtactgc
      121 ccggagtgtg gcacggttca ctggccggac ggagtcgccc cgcccttctg atgcttcccc
      181 gttttgccga tatttttcag cagggaaacc gctggcttaa ctggctggag aaacaaccgg
      241 aaggggcagt ccgtccggtg gtaattgaat cagtgacaaa aatcatggcc tgcgggacca
      301 cactgatggg ctacacgcag tggtgttgtt catcaccgga ctgttgccat acaaaaaagg
      361 tctgcttccg gtgtaaaagc cgctcctgcc cgcactgcgg ggtgaaggct ggcgcacagt
      421 ggatacagta tctgctgagc ctggtccccg actgcccgtg gcagcatatt gtgttcacac
      481 ttccctgcca gtactggtcc ctggtgttcc acaaccggtg gttactggca gagatgagcc
      541 gcattgcagc ggatgtgata ctggaaatct gccatcaggc agatgtggag ccggggatat
      601 tcacggtgag ccacacatgg gggcgtgacc agcagtggca tccgcatatc catttatcga
      661 caactgccgg tggtgtgacg tcgggccaca cctggaaaaa tcttcatttt tacgcccgta
      721 aggtgatgag catgtggcgc taccggataa cacggttgct gtcacggaaa taccctgacc
      781 tggtgatgcc
//
LOCUS       DADMEQ010000316          782 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.318, whole genome shotgun sequence.
ACCESSION   DADMEQ010000316 DADMEQ010000000
VERSION     DADMEQ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.318"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..730
                     /locus_tag="JNZ05_004216"
     CDS             <1..730
                     /locus_tag="JNZ05_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY8567157.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIAWAITTQQKEYQA"
BASE COUNT          225 a          182 c          190 g          185 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
      421 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      481 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      541 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      601 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      661 cgctctggca aataaactgg cccgaattgc ctgggcaatc actactcagc aaaaagaata
      721 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      781 aa
//
LOCUS       DADMEQ010000317          755 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.319, whole genome shotgun sequence.
ACCESSION   DADMEQ010000317 DADMEQ010000000
VERSION     DADMEQ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.319"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..91
                     /gene="yjdI"
                     /locus_tag="JNZ05_004217"
     CDS             <1..91
                     /gene="yjdI"
                     /locus_tag="JNZ05_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAY8567158.1"
                     /translation="WVMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            103..375
                     /locus_tag="JNZ05_004218"
     CDS             103..375
                     /locus_tag="JNZ05_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY8567159.1"
                     /translation="MEICEGHNNFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            602..>755
                     /gene="ghoS"
                     /locus_tag="JNZ05_004219"
     CDS             602..>755
                     /gene="ghoS"
                     /locus_tag="JNZ05_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAY8567160.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLN"
BASE COUNT          235 a          128 c          186 g          206 t
ORIGIN      
        1 gtgggtcatg ccggatgaag tcgacgtcgc cactgtggtt aaagtgattg atacgtgccc
       61 gagcggcgcg ctgaaatacc gtcataaata agcgagggta aaatggaaat atgcgaaggc
      121 cacaataatt tttacattaa tgacgaacaa ggcaagcaaa tcgctgaaat tgtctttgtg
      181 ccgaccggag agaatttagc gattatcgaa cataccgatg tcgatgaaag cctgaaaggg
      241 caagggattg gtaaacagct ggttgcgaaa gtcgtggaaa aaatgcgtcg ggaaaaacga
      301 aaaattatcc cattatgtcc atttgcgaaa cacgaatttg ataaaacgcg ggagtatgat
      361 gatattcgca gttgatggga gagtacagag tcacgatatt tttcttgctc tccgcggtgt
      421 gatgcaggag agcatctgaa aggtaggggg atgcacaaag aatgggcaga gagcgcgttt
      481 ttttgtccca agtcatcccc tttactgagc aaaaaaaaga atatctccta tatgagaatc
      541 atcaatcggg gttaataagt tttgcgtccc cagagcgttt aatattgata ggagtcatat
      601 tatggaaggt aaaaacaagt tcaatactta tgttgtttct tttgattatc catcatctta
      661 ttcctcagtg ttcttaagat taagatcatt gatgtatgat atgaatttct cctctatcgt
      721 ggctgatgaa tatgggatac cacgacaatt gaatg
//
LOCUS       DADMEQ010000318          715 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.320, whole genome shotgun sequence.
ACCESSION   DADMEQ010000318 DADMEQ010000000
VERSION     DADMEQ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.320"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            281..>715
                     /locus_tag="JNZ05_004220"
     CDS             281..>715
                     /locus_tag="JNZ05_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8567161.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDVATNVALQMYLPDGSTSVTPGTETSNIQLA"
BASE COUNT          220 a          122 c          147 g          226 t
ORIGIN      
        1 aaaatgccaa atataaattt tgtgtatctt tttctgaaaa taaactaaag aaagggcttt
       61 atatatcacg catatttatt tgttggatag tcattagatg ttgaatcaat gaattactcg
      121 ttcagttaat atttaaatat tcatgaaatc tataaattaa agatttgtca cttattggat
      181 ttagtatgct tgatttaaat ggcggtcaat tattgttatt attaagtcta atgtcattta
      241 aaggcattat catatttacg aagagatcag gatgatgacg atgaaaaaaa gtgtattgac
      301 ggcgtttata actgtggtat gtgcaacgtc cagcgttatg gctgctgatg ataatgctat
      361 cacggatggc tcagtaacat ttaatggtaa agttattgct ccagcttgta ccctggtagc
      421 tgcgacgaaa gattcagtag tcacattgcc aaatgtcagc gccaccaagt tgcaaactaa
      481 tggagcagtc tctggcgtta aaactgatgt accaattgcc ttagaaggct gtgatgttac
      541 tgtaactaaa aatgcgacgt ttacctttag tggtacggct gacggcgtgc aacctacagc
      601 atttgccaac caggctacca ccgatgtagc gacgaatgtt gctctacaaa tgtatctgcc
      661 agatggttct acatcggtaa ctcccggtac cgaaacgtcg aatattcagt tagcg
//
LOCUS       DADMEQ010000319          702 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.321, whole genome shotgun sequence.
ACCESSION   DADMEQ010000319 DADMEQ010000000
VERSION     DADMEQ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.321"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            247..483
                     /locus_tag="JNZ05_004221"
                     /pseudo
     CDS             247..483
                     /locus_tag="JNZ05_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006685786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            554..>702
                     /locus_tag="JNZ05_004222"
     CDS             554..>702
                     /locus_tag="JNZ05_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY8567162.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDER"
BASE COUNT          193 a          168 c          167 g          174 t
ORIGIN      
        1 ggtgccttta aaattcgttt ttaaaggcta cgatgacatt tttgaagagt cttaaaagaa
       61 ttaaaataca cggcgcgcct atcttgaatg ttttatcgat atcgaagcat tgagcatttt
      121 taactcgttt tatcttgaat gcaatcggcg cataacttat tgaatcccat caaatcccgt
      181 ctttttttac ccattcccgg ttatcttgaa taaattacct agtcatataa cacacttctt
      241 gccgcactgg cattcatcgc ttttcaggca caggcggtga acgtcaccgt ggcgtatcaa
      301 acctcagccg aaccggcgaa agtggctcag gccgacaaca cctttgctaa agagagcggg
      361 gcgagcgtgg actggcgtaa gtttgacagc ggagccagca tcgtgcgggc gctggcttct
      421 ggcgacgtgc aaatcggcaa cctcggttcc agcccattag cggttgcagc cagccaacag
      481 gtgaagtggc acactgaatt tggccacctg aacagaggtg atatgctcac ctcagaacaa
      541 cacaggtgct ccaatgaaaa aaagaaattt tagcgcagag tttaaacgcg aatccgctca
      601 actggttgtt gaccagaaat acacggtggc agatgccgcc aaagctatgg atgttggcct
      661 ttccacaatg acaagatggg tcaaacaact gcgtgatgag cg
//
LOCUS       DADMEQ010000320          694 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.322, whole genome shotgun sequence.
ACCESSION   DADMEQ010000320 DADMEQ010000000
VERSION     DADMEQ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.322"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..65)
                     /locus_tag="JNZ05_004223"
     CDS             complement(<1..65)
                     /locus_tag="JNZ05_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAY8567163.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIAS"
     gene            complement(65..280)
                     /locus_tag="JNZ05_004224"
     CDS             complement(65..280)
                     /locus_tag="JNZ05_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY8567164.1"
                     /translation="MYQMEKISTGIAYGTSGGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          198 a          194 c          132 g          170 t
ORIGIN      
        1 gatgctatag caacagcccc gccaccaata gcaactgcaa cagccttgcg taatgatggc
       61 gacattattc acctctcgca gccttacgct tatcttctct tattttgaaa tacagattcg
      121 tcagataagt cagaagcccc agaagcagac ttcccagcac accaatcgca gcccactgtg
      181 atggactgac ctgatccaac cactgcaaaa accagtagcc ggcactgccg ccggaggtgc
      241 cgtaggcaat gcctgttgat attttttcca tctgatacat atcccgcccc cccccgacag
      301 acctgtgcta tcggaaagaa aaaaggccat cagcaaaact ctgatggcct gaatcacctt
      361 taccaatatt gtatgaaaaa acacgcacga ctaattgaca ataattttca tttccattaa
      421 aaaagatcgc gtagcattct taattcatga ggaactttac ccgccagcaa tctgagtagc
      481 gaaagctgtt catccccaat gtttttgctg gcgggtcctt ttttctacgg tcctcctccc
      541 cggagggggg catttcatta tttttctaat cgttactggt gagaaggcgg aagaaaaagc
      601 cagttcttcg cccctaaata acagcatgat tccagtccat gcaaaatctg atttccagct
      661 cacacatacc gagcgctgta caaaaaaccg ccag
//
LOCUS       DADMEQ010000321          687 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.323, whole genome shotgun sequence.
ACCESSION   DADMEQ010000321 DADMEQ010000000
VERSION     DADMEQ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.323"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            88..153
                     /locus_tag="JNZ05_004225"
                     /pseudo
     CDS             88..153
                     /locus_tag="JNZ05_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            381..629
                     /locus_tag="JNZ05_004226"
     CDS             381..629
                     /locus_tag="JNZ05_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY8567165.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          220 a          141 c          157 g          169 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacacgtaa aatcggtaac ggctggaaat
      661 cattcaatac tcgcactatc gaaagtt
//
LOCUS       DADMEQ010000322          682 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.324, whole genome shotgun sequence.
ACCESSION   DADMEQ010000322 DADMEQ010000000
VERSION     DADMEQ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.324"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            145..>682
                     /locus_tag="JNZ05_004227"
     CDS             145..>682
                     /locus_tag="JNZ05_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HAY8567166.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFY"
BASE COUNT          175 a          181 c          178 g          148 t
ORIGIN      
        1 cgactctttt atcaacgctt acgtaattaa gggacacagc ctaaatagct gcgcggaata
       61 gtagatccct ttgagggaac ttagcccgga ttgtgcgatc tgatcaatcg ccaaatcaaa
      121 acaaatcacc aaccggactg agcaatgccg atcatagcac caatttcccg tgacgaacga
      181 cgcctgatgc agaaagccat ccataaaaca cacgataaaa attatgcccg cagactgact
      241 gccatgctga tgctgcaccg gggcgaccgt gtcagcgacg ttgccagaac gctctgctgc
      301 gcccgttcct cggttggacg ctggattaac tggttcacgc agtcgggtgt tgagggactg
      361 aaatcattac ctgccgggcg tgcccgtcgc tggccgtttg agcatatctg cacactgtta
      421 cgtgagctgg taaaacattc tcccggcgac tttggctacc agcgttcacg ctggagtaca
      481 gaactgctgg caataaaaat caatgagata accggttgcc agttaaatgc cggaaccgtt
      541 cgccgctggt tgccgtctgc ggggattgtg tggcgaaggg ctgcgccaac tctgcgtatc
      601 cgtgacccgc ataaagatga aaagatggca gcaatccata aagcactgga cgaatgcagc
      661 gcagagcatc cggtctttta tg
//
LOCUS       DADMEQ010000323          675 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.325, whole genome shotgun sequence.
ACCESSION   DADMEQ010000323 DADMEQ010000000
VERSION     DADMEQ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.325"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..>675
                     /locus_tag="JNZ05_004228"
     CDS             <1..>675
                     /locus_tag="JNZ05_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY8567167.1"
                     /translation="AARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWK
                     KLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQRRSLVLKAGGKYWHV
                     YMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLDHKTGETATETLTQ
                     RELVARLKQHIPEKFFKMVRYFGFLASRVCGEKLPQVYRALGMDKPEPVAKVCYAQMV
                     KQFLSR"
BASE COUNT          165 a          158 c          224 g          128 t
ORIGIN      
        1 tgccgcccgg aaacgggggc aggaacccgg tatcttctgc gccatccaca cgtatggccg
       61 tcgtctcaac tggcatccgc atgtacatgt gtctgtaacc tgtggaggtc tgaataagca
      121 tggtcagtgg aaaaagctga gcttcctgaa agacgcgatg cgttcgcggt ggatgtggaa
      181 tatgcggcaa cggcttctga aagcgtggtc agaggggctg gcgatgccgg agtcgttgtc
      241 acatatcacg acggaatcac agaggagaag cctggtgctg aaagccggag gaaaatactg
      301 gcatgtgtac atgtcgaaaa aaacggccgg cgggaggaat acggcgcgct acctgggtcg
      361 ttatctgaag aagccgccga tagcggcctc ccggctggca cattacaacg gaggggcgag
      421 cctgagcttc cgttacctgg accacaaaac gggagaaacg gcgacggaaa cgctgacaca
      481 gcgtgagctg gtcgcgaggc tgaaacagca catcccggag aagtttttta agatggtgag
      541 gtacttcggg ttccttgcca gccgggtgtg tggagagaag ctgccgcagg tgtaccgtgc
      601 tctggggatg gataaaccgg aaccagtggc gaaagtgtgc tatgcacaaa tggtgaaaca
      661 gttcctgagt cgtga
//
LOCUS       DADMEQ010000324          669 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.326, whole genome shotgun sequence.
ACCESSION   DADMEQ010000324 DADMEQ010000000
VERSION     DADMEQ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.326"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..>669
                     /locus_tag="JNZ05_004229"
     CDS             <1..>669
                     /locus_tag="JNZ05_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAY8567168.1"
                     /translation="EEVRELIDATPFAKKYTSGVLSFAEKELTPGGREKVMASFERVL
                     MPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLV
                     NHHYGLHDPNAPENRRTLTLPDNLPETKQALAESVTRGIDALYHVGEIKGRQDVIQAL
                     TEAGIDVVRVTRSSISIADPNGGKNVRLKGAFYEQSFTDGCGVREKAERESRIYRENA
                     ERRV"
BASE COUNT          188 a          156 c          206 g          119 t
ORIGIN      
        1 ggaagaagtc cgggaactca tcgatgccac gccatttgcg aagaaataca cgtccggtgt
       61 tctgtcgttc gcggagaagg agctgacgcc gggaggacgt gaaaaagtga tggcgagctt
      121 tgagcgtgta ctgatgcccg gtctcgaaaa aaatcagtac agcatcctgt gggtggagca
      181 ccaggacaag ggacggcttg agctgaattt tgtcattccg aacatggagc tacagaccgg
      241 aaaacgcctc cagccgtact acgaccgcgc agacaggcct agaattgatg cctggcagac
      301 gctggttaat caccattacg ggctgcatga cccgaacgcc ccggagaacc gcaggacgct
      361 gacactccct gataatctgc cggaaacgaa acaggcgctt gcagagagcg tcacacgcgg
      421 gatagacgct ctttatcatg tcggggagat aaaaggccgt caggatgtga ttcaggcgct
      481 cacagaggca gggattgacg tggtcagggt gacgcgaagc agtatcagca ttgcagaccc
      541 gaacggcggg aagaatgtca gactgaaagg agcgttttat gagcaatctt ttacagacgg
      601 gtgcggagtt cgagaaaaag ctgaaagaga gagccgaatc tacagagaaa atgctgaacg
      661 acgagttca
//
LOCUS       DADMEQ010000325          660 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.327, whole genome shotgun sequence.
ACCESSION   DADMEQ010000325 DADMEQ010000000
VERSION     DADMEQ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.327"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..>660
                     /locus_tag="JNZ05_004230"
     CDS             <1..>660
                     /locus_tag="JNZ05_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY8567169.1"
                     /translation="HAGEAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCG
                     FDDDLAAQTTQASNRIRGLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLGE
                     KKLAAQLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRD
                     EVALEVEQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVT
                     R"
BASE COUNT          132 a          226 c          173 g          129 t
ORIGIN      
        1 tacacgccgg tgaagctaaa actgatgctc gtgacgctgc catcattgcc gaagctgccc
       61 gtaccctgcc tcacgcgcta cgcacgctga aactggctga cgagcaaatc gccgaactct
      121 ccatgctctg cggcttcgat gatgatcttg ccgcacagac aacgcaggcc agcaaccgta
      181 tccgcggcct tctgacccag atacatccgg cactggagcg cgttctcggt ccgagacttg
      241 agcacccggc ggtactcgat cttctccagc gatatccctc accagaaaaa ctcgcttcgc
      301 tgggtgagaa gaagctggca gcccagctct gcaaacttgc gcctcgtctg ggtaaacgcc
      361 ttgcagcaga catagctcag gcactggccg aacaaaccgt cgtcgttccc ggcacgaatg
      421 ccgctgccgt agtactgcca cgtctggcac tccagctcat cacgctgcgt aagcaaagag
      481 acgaggtggc gcttgaggta gaacagcgag ttcttgctca ccctctttac ccggtcctga
      541 ccagtatgcc cggagtcggt gtcaggaccg cagccagact cctcaccgag gtcgcctgcc
      601 gcgccttcgc ctctgccgca catctcgctg cttatgctgg ccttgcgccg gtaactcggc
//
LOCUS       DADMEQ010000326          658 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.328, whole genome shotgun sequence.
ACCESSION   DADMEQ010000326 DADMEQ010000000
VERSION     DADMEQ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.328"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(151..657)
                     /locus_tag="JNZ05_004231"
     CDS             complement(151..657)
                     /locus_tag="JNZ05_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY8567170.1"
                     /translation="MPVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGND
                     LKELRVALNYSQNPVSPKARSLILWTERGAARHAKMLETDQAWDVFEKLEDCYFSQRD
                     PSTPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDK
                     LVERLMQL"
BASE COUNT          173 a          160 c          146 g          179 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc ggaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttggcgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaatc
//
LOCUS       DADMEQ010000327          654 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.329, whole genome shotgun sequence.
ACCESSION   DADMEQ010000327 DADMEQ010000000
VERSION     DADMEQ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.329"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..286)
                     /locus_tag="JNZ05_004232"
     CDS             complement(<1..286)
                     /locus_tag="JNZ05_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY8567171.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNT"
     gene            complement(299..466)
                     /locus_tag="JNZ05_004233"
     CDS             complement(299..466)
                     /locus_tag="JNZ05_004233"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY8567172.1"
                     /translation="MRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSVNPGLV
                     RASATFTLEFQ"
     gene            complement(454..>654)
                     /locus_tag="JNZ05_004234"
     CDS             complement(454..>654)
                     /locus_tag="JNZ05_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567173.1"
                     /translation="FPTTGSTSPAVPFQITFSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGKKYWTEICVR"
BASE COUNT          166 a          157 c          151 g          180 t
ORIGIN      
        1 ccgtattgtc agttgaacct gtcacgattg ccgtcactgc acttgtttca accggacaat
       61 cggttaatga caaagtaata ttgtgccagc cagatgcaga accaggttgt tgcagattgc
      121 gcgtataaag atccccgaga ttaacgttag cttctttggt ttgaatagtg cagggtttag
      181 cgactacccg accattaaca gtgatagtga catcggcggc ctggagagtt gctgtgaata
      241 acgcgcaaaa cgtagctaac aggaacctct tgtgaagtct tttcatatct gactctgatt
      301 attgaaattc aagggtaaag gttgccgaag ccctcaccag tcccggattg acggacttct
      361 gagttgactt cagacgagcg gagtaaggca aaatattgtt ctgttctggt accagtggga
      421 tccactgcat cccggcatga aggtcattca gtttcaccgg acgcatattt ccgtccagta
      481 tttcttaccc aaaccggagg ccgtattgct tccctcatcc agtttcaaca gagtattatt
      541 ttctgcatcc tcaataccgt taaatgcaac ccgaaccccc gttgtccctt tgctgcattc
      601 actaaacgta atctgaaaag ggacggctgg acttgtgcta ccggtcgttg gaaa
//
LOCUS       DADMEQ010000328          630 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.330, whole genome shotgun sequence.
ACCESSION   DADMEQ010000328 DADMEQ010000000
VERSION     DADMEQ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.330"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..222
                     /locus_tag="JNZ05_004235"
     CDS             <1..222
                     /locus_tag="JNZ05_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY8567174.1"
                     /translation="PQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTVL
                     DGKVIDERTTSHINRMQEDTLISSAYPEE"
BASE COUNT          218 a          121 c          115 g          176 t
ORIGIN      
        1 ccacagtttt cacctgaagc attaaataat ctgaaaggtc gagtggaaac attcacacaa
       61 gatttaaagg ttatcaaacc gtcagtaaat attcagcctc agacaatcaa tatcacaact
      121 cagacggtac ttgatggaaa ggttattgat gaaagaacca catctcatat taatcgtatg
      181 caggaagata ccttgattag ctcagcttac ccagaggaat aaacaatgtt agaactacta
      241 atatttattg ccacacatgg caatctatta atctttatct agtatgacga ttcaactatg
      301 aatagtcgaa tgctacgtga tttcttctga gcagatacca acatcaaaaa ttagatttca
      361 cgtagctcac gcttaacaga ataggaataa caacaatgtt tgacacaaag caaattagat
      421 actcaggtaa agatggtatc tattttcacc ttagagataa tgtagaatct tttcttgcct
      481 taacagctac tgaacagatg gactatgact caccgatgat ggtgacaact caggctatgc
      541 aatcggggca aactatcact gataatgttc agagagcacc taagaccatc tctattagcg
      601 gtgttgtagt ggttgattcg acgggttcag
//
LOCUS       DADMEQ010000329          613 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.331, whole genome shotgun sequence.
ACCESSION   DADMEQ010000329 DADMEQ010000000
VERSION     DADMEQ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.331"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(12..>613)
                     /gene="ospZ"
                     /locus_tag="JNZ05_004236"
     CDS             complement(12..>613)
                     /gene="ospZ"
                     /locus_tag="JNZ05_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HAY8567175.1"
                     /translation="HGYFNESPNIYDKKYISGITRSMAQLKIEEFINEKSRRLNYMKT
                     MYSPCPEDFQPISRDEASTPEGSWLTVISGKRPMGQFSVDSLYHPDLHALCELPEISC
                     KIFSKENSYFLYIIVVFRNDSPQGELRANRFIELYDIKREIMQVLRDESPELKSIKSE
                     IIIAREMGELFSYASEEIDSYIKQMNDRLSQIKARMPVT"
BASE COUNT          186 a          121 c           91 g          215 t
ORIGIN      
        1 attaatatac attaagtaac aggcattcga gccttgattt gagaaagcct gtcattcatt
       61 tgttttatgt agctatctat ttcctctgat gcataactaa aaagttctcc catttcacga
      121 gcaataataa tctcagattt aatagacttt aactctggcg actcatctct taacacttgc
      181 ataatctctc tttttatatc atatagttct ataaaccgat ttgcccgtag ttctccttgg
      241 ggagaatcat ttctgaatac aacaattata tacaaaaaat aactattttc tttagagaag
      301 atcttacaac taatctctgg gagctcacat aatgcatgta agtcaggatg gtacaaacta
      361 tccacagaaa attgccccat aggacgtttt ccagatataa ctgtcaacca gcttccttcc
      421 ggtgtactgg cttcgtctct ggaaataggc tgaaaatctt ccggacatgg agaatacatt
      481 gtcttcatat aattaagtcg tctggatttc tcattaataa actcctctat ttttaattgt
      541 gccatacttc tggttatacc agagatatac ttcttgtcat atatgtttgg tgattcatta
      601 aagtaaccat gtt
//
LOCUS       DADMEQ010000330          596 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.332, whole genome shotgun sequence.
ACCESSION   DADMEQ010000330 DADMEQ010000000
VERSION     DADMEQ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.332"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..323
                     /locus_tag="JNZ05_004237"
     CDS             <1..323
                     /locus_tag="JNZ05_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8567176.1"
                     /translation="ARIAREHDINNNLLFKWKSQYEDGLLSDDDIQECMPVPVALTDT
                     PEPTRPVTNPFWRNKHDERPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMK
                     GGIR"
     gene            320..>596
                     /gene="tnpB"
                     /locus_tag="JNZ05_004238"
     CDS             320..>596
                     /gene="tnpB"
                     /locus_tag="JNZ05_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY8567177.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQ"
BASE COUNT          145 a          157 c          172 g          122 t
ORIGIN      
        1 ttgcccggat cgccagagaa cacgatatca ataataacct gctgtttaaa tggaaaagcc
       61 agtacgagga cggcttactg agcgatgatg acatacagga atgcatgcct gtcccggtgg
      121 cactgactga tacgccggag ccgaccagac cagttacaaa tcccttctgg cgtaacaagc
      181 atgatgagcg ccctgagggg gctcccggaa acgtcccacg gtgcgagctg catcttaaat
      241 caggtgtggt aaaactgttt gaccctctca ctccggaact gttacgggcg ctaatccgcg
      301 aaatgaaagg gggtatccga tgataacgct gccgaccggt accaaaatct ggatcatcgc
      361 tggcatcaca gatatgcgtt gtggcttcaa tggcctggct tcgaaggtgc agaacacgct
      421 gaaagatgac ccgttctccg ggcatatctt cgtcttccgg ggccgcagtg gcaaaatggt
      481 gaaaatactg tgggccgatc gtgacgggtt atgcctgttc gccaaacgcc tggaacgggg
      541 ccgcttcgtc tggccggtga cccgggaagg gaaagtgcac ctgacgccag ctcagt
//
LOCUS       DADMEQ010000331          575 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.333, whole genome shotgun sequence.
ACCESSION   DADMEQ010000331 DADMEQ010000000
VERSION     DADMEQ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.333"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..>575
                     /locus_tag="JNZ05_004239"
     CDS             <1..>575
                     /locus_tag="JNZ05_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8567178.1"
                     /translation="GGQQECEMRRFAGACRFVFNRALARQNENHEAGNKYIPYGKMAS
                     WLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPRFKKRGQNDAFRYP
                     QGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWYISIQTESEVSTPA
                     HPSASMVGLDAGVAKLATLSDGTVFEPVNSF"
BASE COUNT          146 a          140 c          154 g          135 t
ORIGIN      
        1 ggtggtcaac aggagtgtga aatgaggcgc ttcgcaggcg catgtcgttt cgttttcaat
       61 cgtgctctgg cacgtcagaa tgagaatcat gaggccggta ataaatacat cccttacggg
      121 aaaatggctt cctggctggt tgagtggaaa aatgccactg aaacgcaatg gcttaaagat
      181 tctccctcac agccattgca acagtcactg aaagaccttg agcgggctta caaaaacttc
      241 ttccggaagc gggctgcttt tccccgattc aaaaagcggg gacagaatga tgcattccgc
      301 tacccgcagg gcgttaagct cgatcaggaa aacagccgta tttttctgcc gaaactgggc
      361 tggatgcgct accggaatag ccgtcaggtc acgggtgttg tgaaaaatgt cactgtcagc
      421 cagtcctgcg gtacgtggta catcagtatt cagacagaaa gtgaagtatc cactccggct
      481 cacccttcag catcaatggt cgggctggat gctggcgtgg ctaaactcgc cacgctgtca
      541 gatggcacag tctttgagcc tgtaaacagt tttca
//
LOCUS       DADMEQ010000332          572 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.334, whole genome shotgun sequence.
ACCESSION   DADMEQ010000332 DADMEQ010000000
VERSION     DADMEQ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.334"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..276
                     /locus_tag="JNZ05_004240"
     CDS             <1..276
                     /locus_tag="JNZ05_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY8567179.1"
                     /translation="LNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGFDNGGESAA
                     IIYSLLFTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
BASE COUNT          140 a          127 c          150 g          155 t
ORIGIN      
        1 ctgaacgtgt actgtcgtaa tggccaggtg gaaatcgaca acaacatcgg tgaaaacgcg
       61 ctgcgatcgg tggcggttgg aagaaaaaat tatctcttct tcggttttga caacggtgga
      121 gaaagcgcgg cgattatcta cagtctgctg ttcacctgca aactgaacgg agtggagccg
      181 gaggactggt tgcgcgaagt gatcgtgaag ctcaatgact ggccgtcgaa ccgagtgcat
      241 gaactgctgc cctggaactt ctcagctgta aaataatcct tccgctacgt acttctctgg
      301 gcgcttacca ctcaacggca tcgtcaaaat tatcaaagcg cagaacatag atttatcatg
      361 tgttttatgg atggctttct ccatcaggca tcgttcgtca cgggaaattg gtgctatgat
      421 cggcattgat cagtccggtt ggtgatttgt tttgatttgg cgattgatca gatcgcacaa
      481 tccgggctga gttccctcta agtgatctag gttgtgtccc ttaattacgt aagcgttgat
      541 aaaagagtcg gatgcagccc agtttcacca tc
//
LOCUS       DADMEQ010000333          571 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.335, whole genome shotgun sequence.
ACCESSION   DADMEQ010000333 DADMEQ010000000
VERSION     DADMEQ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.335"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
BASE COUNT          151 a          160 c          125 g          135 t
ORIGIN      
        1 ctcaccattc cctcgcgata ttaacgtaat atcttaattg tatgcaccgc cagaatgcgt
       61 tcagtgcgat cacggcagca gacaggtaaa aatagcaaca aaccacccga aaaatcgccg
      121 cgatcgcgta tgataaattt taaccacatg catacctgtg cagcaaggcg aatcacgcac
      181 ggatttccag ccagagacac ctgaaacgta accggctcat ttaaaccgtc tggtctgtta
      241 cctccggttc cacaaaaata atgtccatca ttttgaagtg gacactatcg tatggaatac
      301 cggacctgaa tcactgaagc tttacgcctt catttcgaag aacatttacc acgggttgtg
      361 gccggacgtc gcctgggcgt accaaaatca actgtctgtg gtatgttcgt gcgcttccgc
      421 aacgctggcc tttcatggcc tctgcccgca ggtatgtcgg agcaggaact tgatgccctt
      481 ctttacggaa gtgcctccac agtacctgtc gtacttactg aaagcaccgt tatgcccaaa
      541 ctcccggtgg taaaaaaacg tccccggcgg c
//
LOCUS       DADMEQ010000334          566 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.336, whole genome shotgun sequence.
ACCESSION   DADMEQ010000334 DADMEQ010000000
VERSION     DADMEQ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.336"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..297
                     /locus_tag="JNZ05_004241"
     CDS             <1..297
                     /locus_tag="JNZ05_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HAY8567180.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="JNZ05_004242"
     CDS             complement(405..>566)
                     /locus_tag="JNZ05_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567181.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DADMEQ010000335          553 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.337, whole genome shotgun sequence.
ACCESSION   DADMEQ010000335 DADMEQ010000000
VERSION     DADMEQ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.337"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(28..>553)
                     /locus_tag="JNZ05_004243"
     CDS             complement(28..>553)
                     /locus_tag="JNZ05_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY8567182.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGAAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT          144 a          139 c          134 g          136 t
ORIGIN      
        1 accagtattt cttcggagtg aagaatatta ccaagtatat ttaacaccca cgttcgcgga
       61 ccagtcttga tctacgtcac caccaccgag gtagttagca tcggtatagg cgctgaagtt
      121 cttggtgaag ctgaactgag tacccagacc aacacgtacc gcagcccctt cagtaccgtt
      181 atcgatggaa tcaccgttca catcgttatc gttgttagag tcgtcgtaga cgtaagccag
      241 tttgaagtac ggagtcagag cctgatcttc gctgtaggtg aaggtataac ctgcatctac
      301 acccagttca taacgcatgc tgtcgtaaga ctgaccgtca actttcatgt cgttgctcag
      361 ctggtagtca tcaccagact ggaacagacc agaaatgctg ccgtaaggag tcacgtaacc
      421 agcatcaccc agtttgaagt cgtaaccggc tttcaaaccg aagccccaag cgtcggagtt
      481 ggtgctaccg tcaacgtaag taccgttgct catggttgca gacaggtcgt tgttgaagtg
      541 agagtagctc aag
//
LOCUS       DADMEQ010000336          542 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.338, whole genome shotgun sequence.
ACCESSION   DADMEQ010000336 DADMEQ010000000
VERSION     DADMEQ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.338"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(87..236)
                     /gene="hokB"
                     /locus_tag="JNZ05_004244"
     CDS             complement(87..236)
                     /gene="hokB"
                     /locus_tag="JNZ05_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HAY8567183.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(336..476)
                     /locus_tag="JNZ05_004245"
                     /pseudo
     CDS             complement(336..476)
                     /locus_tag="JNZ05_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          140 a          127 c          126 g          149 t
ORIGIN      
        1 acatgcacga actgaagtgt aaaaggggtg ccatgagaaa atgaatctgc tagtcagatg
       61 cgcggggaaa atccccgcgc ttgcccttac ctggacgtgc aggccatgag cgcagcaacc
      121 tccttatcac cgtcccggaa ccgcagttcg tagagcgttt gtcgggtcag gagtgtgaat
      181 gccagaatcg taatgcagat aatgagcaga cacaccacca gagggttgcg cttcatagcc
      241 tttctccttg ccggatggcg ggtaagaggc taacatctga attgcttggt tcattcgttg
      301 gcctcggttt gtactgaccc caaaaagttg gacagttaaa cacgaggcat ataggtctga
      361 ttccgatatt catttggagt cagacctttt aatttcaggc taattcttct gctgttgtag
      421 tattcaatat attccgtaac agcatccttc agttcgctta tattactgaa ctcattagac
      481 tggccccctg aatctccaga caaccagtat cacttattta agtgatagtc ttaatactag
      541 tt
//
LOCUS       DADMEQ010000337          525 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.339, whole genome shotgun sequence.
ACCESSION   DADMEQ010000337 DADMEQ010000000
VERSION     DADMEQ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.339"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..>525)
                     /locus_tag="JNZ05_004246"
     CDS             complement(<1..>525)
                     /locus_tag="JNZ05_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY8567184.1"
                     /translation="CADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNNAADTHVV
                     DVYNIGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNSSEVFNNTI
                     TVKDSTVTSGSWTDEGTTGWFGNTGNASDYSGKSNFVTVDTDGDGVADSTIASWDDVA
                     LAVVAHPNADNAMQ"
BASE COUNT          149 a          124 c          105 g          147 t
ORIGIN      
        1 tctgcatagc attatcagca ttcggatgtg caactacagc taatgcaaca tcatcccagc
       61 ttgcaatagt gctatcagca acaccatctc cgtcagtatc tacggtgaca aagttagatt
      121 taccactata atcgctggca ttacctgtat taccgaacca accagtagta ccttcatcag
      181 tccatgaacc agaggttacg gtagaatctt taacagtgat ggtgttatta aacacttcgc
      241 tgctgtttac gccagtgctg acagtgttgt cgtcaatatc ttcccactca taaccctgag
      301 tcaacgtaat acccgcaacg tgagagttat tagtgatgga cagatcaact tcctgatcca
      361 gagtaatagc cgtaccaatg ttgtaaacgt ctacaacatg agtatcagcg gcattattgt
      421 aagtgccgtt atatgtgtag tgttcgtagt tgtcgtcgat cgttgaatta tcaacagtca
      481 gcgccagacg gtcatggtag taatcatccg cacgatcagc acaat
//
LOCUS       DADMEQ010000338          525 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.340, whole genome shotgun sequence.
ACCESSION   DADMEQ010000338 DADMEQ010000000
VERSION     DADMEQ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.340"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..189)
                     /locus_tag="JNZ05_004247"
     CDS             complement(<1..189)
                     /locus_tag="JNZ05_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAY8567185.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQT"
     gene            254..445
                     /locus_tag="JNZ05_004248"
     CDS             254..445
                     /locus_tag="JNZ05_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY8567186.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
BASE COUNT          128 a          159 c          136 g          102 t
ORIGIN      
        1 tgtctgattc aggttattcc cgatggcagc cagctgacgc agtaacggcg gtgccagtgt
       61 cggcagtttc ccggaacggg caaccggctc acccaggcag acccgccgca tccataccgc
      121 cagctgttta ccctcacagc gttccagtaa ccgggcatgt tcatcatcag taacccgtat
      181 ggtgagcatc ctctcgcgat tcatcggtat cattacccca taaaacagaa atccccctta
      241 cacggaggca tcagtgacta aacaggaaaa aaccgccctt aacatggccc gctttatcag
      301 aagccagaca ttaacgctgc tggagaagct caacgaactg gacgcggatg aacaggctga
      361 tatctgtgaa tcgcttcacg accacgccga tgagctttac cgcagctgcc tcgcacgttt
      421 cggggatgac ggtgaaaacc tctgacatat gcagctcccg gagacggtca cagcttgtct
      481 gtgagcggat gccgggagca gacaagcccg tcagggcgcg tcagc
//
LOCUS       DADMEQ010000339          514 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.341, whole genome shotgun sequence.
ACCESSION   DADMEQ010000339 DADMEQ010000000
VERSION     DADMEQ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.341"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..>514
                     /locus_tag="JNZ05_004249"
     CDS             <1..>514
                     /locus_tag="JNZ05_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HAY8567187.1"
                     /translation="RWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQI
                     DMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKA
                     PTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESI
                     EMVRRYAHLAP"
BASE COUNT          150 a          111 c          143 g          110 t
ORIGIN      
        1 cgctggctgg agccccatga agcacaaagg ctgattgatg aatgtccgga gccattaaag
       61 tctgttgttg aatttgcact ggcaacaggc ttaagacgct cgaacatcat caaccttgaa
      121 tggcaacaaa tagatatgca gcgccgggtg gcatggataa acccggaaga gagtaaatca
      181 aaccgcgcaa tcggcgttgc gctgaatgat actgcatgtc gcgttttgaa aaaacaaatc
      241 ggaaatcatc accgttgggt atttgtgtac aaggaaagct gtaccaaacc agacggaacg
      301 aaagcgccaa cagtaaggaa gatgcggtat gacgcaaaca cagcctggaa agcggcgctg
      361 agacgggctg gtattgatga tttcagattt cacgacttga gacacacctg ggcaagttgg
      421 ctggttcaag ccggagtccc gttgtcagtg ttacaggaaa tgggaggctg ggagtctatc
      481 gaaatggttc gtcgatatgc tcaccttgca ccta
//
LOCUS       DADMEQ010000340          513 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.342, whole genome shotgun sequence.
ACCESSION   DADMEQ010000340 DADMEQ010000000
VERSION     DADMEQ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.342"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..>513)
                     /locus_tag="JNZ05_004250"
     CDS             complement(<1..>513)
                     /locus_tag="JNZ05_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY8567188.1"
                     /translation="REGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGEVGQTAPNVL
                     QRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVENML
                     DQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFG
                     TLKSECFYLD"
BASE COUNT          133 a          121 c          102 g          157 t
ORIGIN      
        1 catcaagata aaaacactcc gactttaagg ttccaaagaa acactccacc acagcattat
       61 ccagacaatt gccttttctg gacatgcttt gtttaatacc atgttcttta aggatatttt
      121 gatatcttct catacgatac tgccatccct ggtcagagtg cagaacagga tgctcgtgag
      181 gattaagctt tttgaatgcc tgatcgagca tattctcaac catgttcatc actggtcttt
      241 ccgaaaggct gtaagaaata acttcgttgt tgaagagatc tattactgga gacaaataca
      301 gcttgcgccc attgactgca aattcagtaa catcggtaac ccacttctcg tttggccgcg
      361 tagccttgaa atctctttgg agaacattag gggcggtttg ccctacctct cctctgtaag
      421 agcggtatcg cttgacctta atcgctgctt taagtgagag ggttcccatc aggcgctgaa
      481 cagctttatg gttaatctgt ttcccttctc gat
//
LOCUS       DADMEQ010000341          513 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.343, whole genome shotgun sequence.
ACCESSION   DADMEQ010000341 DADMEQ010000000
VERSION     DADMEQ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.343"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..502
                     /locus_tag="JNZ05_004251"
     CDS             <1..502
                     /locus_tag="JNZ05_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY8567189.1"
                     /translation="PTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKARSVQLMQS
                     LYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDNNIGENALRSVAVG
                     RKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLPW
                     NFSSVK"
BASE COUNT          149 a          109 c          153 g          102 t
ORIGIN      
        1 tccgacagaa atgactcagg aagcgctcag acggatagca gagttatacg acatagaagc
       61 ggagatacgt ggcagtccgg cagaggaacg gcttgcagtc agaaaagcca gaagcgtcca
      121 gttgatgcag tcgttgtacg actggataca gttgcagagg aaaacgctgt cgaaacatgc
      181 ggagatggcg aaggcgttcg actatatcct gaatcactgg aatgcgctga acgagttctg
      241 tcgtgacggc tgggtggaaa tagacaacaa catcggtgaa aacgcgttac gatcggtggc
      301 ggttggaaga aaaaattatc tctttttcgg ctcagacaag ggaggagaaa gtgcggcgat
      361 catctacagt ctgctggtca cctgcaaaca gaacgaagtg gagccggagg actggttgcg
      421 cgaagtgatc gagaagctca atgactggcc gtcgaaccaa gtgcatgaac tgctgccctg
      481 gaacttctcg tctgtaaaat aatccttacg cta
//
LOCUS       DADMEQ010000342          512 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.344, whole genome shotgun sequence.
ACCESSION   DADMEQ010000342 DADMEQ010000000
VERSION     DADMEQ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.344"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            43..512
                     /locus_tag="JNZ05_004252"
                     /pseudo
     CDS             43..512
                     /locus_tag="JNZ05_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          130 a          130 c          131 g          121 t
ORIGIN      
        1 gacacctcag agtcatttaa gatggcttaa agagaggtgc ccatgagcgg taagcgttat
       61 cccgaagagt ttaaaactga agcagtcaaa caggttgttg atcgcggtta ttctgttgcc
      121 agcgttgcaa cacgtctcga tatcaccacc cacagccttt atgcctggat aaagaagtac
      181 ggtccggatt cttccactaa taaagaacag tcagatgctc aggccgagat ccgccgtctc
      241 cagaaagagc tgaaacgggt taccgacgaa cgggacatat taaaaaaagc cgcggcgtac
      301 ttcgcaaagc tgtccgactg aggtacgcct ttatccgtga caactcctgt tgctggcctg
      361 ttcgcctgct ctgtcgggtg ctggatgttc atcccagtgg tttttacgcc tggcttcagc
      421 agccgcattc acaacgccat caggcagacc tgagactgac aggacagatt aaacagttct
      481 ggctggaatc gggatgcgtc tatggttatc gc
//
LOCUS       DADMEQ010000343          509 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.345, whole genome shotgun sequence.
ACCESSION   DADMEQ010000343 DADMEQ010000000
VERSION     DADMEQ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.345"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..>509
                     /locus_tag="JNZ05_004253"
     rRNA            <1..>509
                     /locus_tag="JNZ05_004253"
                     /product="23S ribosomal RNA"
BASE COUNT          132 a          116 c          148 g          113 t
ORIGIN      
        1 ggtgccgtaa cttcgggaga aggcacgctg atacgtaggt gaagcgactt gctcgtggag
       61 ctgaaatcag tcgaagatac cagctggctg caactgttta ttaaaaacac agcactgtgc
      121 aaacacgaaa gtggacgtat acggtgtgac gcctgcccgg tgccggaagg ttaattgatg
      181 gggttagcgc aagcgaagct cttgatcgaa gccccggtaa acggcggccg taactataac
      241 ggtcctaagg tagcgaaatt ccttgtcggg taagttccga cctgcacgaa tggcgtaatg
      301 atggccaggc tgtctccacc cgagactcag tgaaattgaa ctcgctgtga agatgcagtg
      361 tacccgcggc aagacggaaa gaccccgtga acctttacta tagcttgaca ctgaacattg
      421 agccttgatg tgtaggatag gtgggaggct ttgaagtgtg gacgccagtc tgcatggagc
      481 cgaccttgaa ataccaccct ttaatgttt
//
LOCUS       DADMEQ010000344          506 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.346, whole genome shotgun sequence.
ACCESSION   DADMEQ010000344 DADMEQ010000000
VERSION     DADMEQ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.346"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
BASE COUNT          170 a           95 c          112 g          129 t
ORIGIN      
        1 gctgatgggg cgcttacatt aatcccgtac tctctggcca gttgggccac gcaggcccct
       61 agctggtaag atttttcaat aagatcaagc ttaaactcca tagagtcata gagtaagagg
      121 cacgaatcct gcgggggcga agagaatctg acattgatag agtccattaa cgttataaga
      181 ggactctatc atcgccaatc atcagacatt gaaatagacg acactgagat gacgcttaca
      241 attcttttgg tttctgtaat aagtaatctg acattccccg tgcccagaag aggagaggga
      301 tatcaagccc aagggttaat aattgatact acggttaaaa ttgccttcag taaaggaaac
      361 gtgattaaat tcaggggatt tcagagtgaa aaccatacaa catattaaaa tctgttcatt
      421 tagctataat acatacaaga aagtgtccag ttagataatg ttatctaaat aaccacagat
      481 aaaaacgcac ataattgggg gggggg
//
LOCUS       DADMEQ010000345          496 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.347, whole genome shotgun sequence.
ACCESSION   DADMEQ010000345 DADMEQ010000000
VERSION     DADMEQ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.347"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            13..>496
                     /locus_tag="JNZ05_004254"
     CDS             13..>496
                     /locus_tag="JNZ05_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8567190.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     Y"
BASE COUNT          122 a          126 c          127 g          121 t
ORIGIN      
        1 tcaggagagc ttatggtcac ttttgagaca gttatggaaa ttaaaatcct gcacaagcag
       61 ggaatgagta gccgggcgat tgccagagaa ctggggctct cccgcaatac cgttaaacgt
      121 tatttgcagg caaaatctga gccgccaaaa tatacgccgc gacctgctgt tgcttcactc
      181 ctggatgaat accgggatta tattcgtcaa cgcatcgccg atgctcatcc ttacaaaatc
      241 ccggcaacgg taatcgctcg cgagatcaga gaccagggat atcgtggcgg aatgaccatt
      301 ctcagggcat tcattcgttc tctctcggtt cctcaggagc aggagcctgc cgttcggttc
      361 gaaactgaac ccggacgaca gatgcaggtt gactggggca ctatgcgtaa tggtcgctca
      421 ccgcttcacg tgttcgttgc tgttcccgga tacagccgaa tgttgtacat cgaattcact
      481 gacaatatgc gttatg
//
LOCUS       DADMEQ010000346          495 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.348, whole genome shotgun sequence.
ACCESSION   DADMEQ010000346 DADMEQ010000000
VERSION     DADMEQ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.348"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..>495)
                     /locus_tag="JNZ05_004255"
     rRNA            complement(<1..>495)
                     /locus_tag="JNZ05_004255"
                     /product="16S ribosomal RNA"
BASE COUNT           92 a          165 c          109 g          129 t
ORIGIN      
        1 aatcctgttt gctccccacg ctttcgcacc tgagcgtcag tcttcgtcca gggggccgcc
       61 ttcgccaccg gtattcctcc agatctctac gcatttcacc gctacacctg gaattctacc
      121 cccctctacg agactcaagc ttgccagtat cagatgcagt tcccaggttg agcccgggga
      181 tttcacatct gacttaacaa accgcctgcg tgcgctttac gcccagtaat tccgattaac
      241 gcttgcaccc tccgtattac cgcggctgct ggcacggagt tagccggtgc ttcttctgcg
      301 ggtaacgtca atgagcaaag gtattaactt tactcccttc ctccccgctg aaagtacttt
      361 acaacccgaa ggccttcttc atacacgcgg catggctgca tcaggcttgc gcccattgtg
      421 caatattccc cactgctgcc tcccgtagga gtctggaccg tgtctcagtt ccagtgtggc
      481 tggtcatcct ctcag
//
LOCUS       DADMEQ010000347          464 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.349, whole genome shotgun sequence.
ACCESSION   DADMEQ010000347 DADMEQ010000000
VERSION     DADMEQ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.349"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..>464
                     /locus_tag="JNZ05_004256"
     CDS             <1..>464
                     /locus_tag="JNZ05_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY8567191.1"
                     /translation="QHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEHWGYVGAKSRQ
                     RWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKL
                     HVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHY"
BASE COUNT          113 a          111 c          133 g          107 t
ORIGIN      
        1 ctcaacacgg ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac
       61 aaccgggcag tgatgtgatt gtctgcgctg aaatggacga acattggggc tacgtcggtg
      121 ctaaatcacg tcagcgctgg ctgttttacg cgtatgacag gatacggagg acggttgtgg
      181 cgcacgtctt cggtgaacgc actctggcca cactggagcg tcttctgagc ctgctgtcgg
      241 cctttgaggt cgtggtatgg atgacggatg gctggccgct gtatgaatca cgcctgaagg
      301 gaaagctgca cgttatcagc aagcgttaca ctcagcgcat tgagcgacat aatctgaatc
      361 tgagacaaca tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc
      421 tgcatgacaa ggtcatcggg cattatctga acataaaaca ctat
//
LOCUS       DADMEQ010000348          462 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.350, whole genome shotgun sequence.
ACCESSION   DADMEQ010000348 DADMEQ010000000
VERSION     DADMEQ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.350"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..183
                     /locus_tag="JNZ05_004257"
     CDS             <1..183
                     /locus_tag="JNZ05_004257"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567192.1"
                     /translation="QLEKTGITKDAIRKDMVPALRELMQAKHRAQKAVDELVDCVAEL
                     ETRVGKFEKLVQEALR"
     gene            278..>462
                     /locus_tag="JNZ05_004258"
     CDS             278..>462
                     /locus_tag="JNZ05_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HAY8567193.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRG"
BASE COUNT          137 a           91 c          136 g           98 t
ORIGIN      
        1 caactcgaaa aaacgggcat aacaaaagat gctatccgta aagatatggt acccgcgctt
       61 cgggaactga tgcaggcgaa gcatcgcgca cagaaagcag tagatgagct ggtggactgt
      121 gtggcagagc tggaaaccag agtcggaaag tttgaaaaac tggtgcagga ggcgctgcgc
      181 tgatgcgcca tgagtttgtt ttgccctggc ttgtgcgcga tcattatgtc cctggtggaa
      241 agcccgggat aaaaatcacc gaaccggaga gtatttgatg agcgtcaaaa ttcaaacgat
      301 accggaactg cttatccaga cacgaggtaa tatgaccgaa gtttcacgaa tgctgaactg
      361 taatcgcgcc acggtaagaa aatatgctga agataaagag ggcaaagggc acgccattgt
      421 tgatggtgtt ctgattgttc atcgtggatg ggacaggggt aa
//
LOCUS       DADMEQ010000349          459 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.351, whole genome shotgun sequence.
ACCESSION   DADMEQ010000349 DADMEQ010000000
VERSION     DADMEQ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.351"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..>459)
                     /locus_tag="JNZ05_004259"
     rRNA            complement(<1..>459)
                     /locus_tag="JNZ05_004259"
                     /product="23S ribosomal RNA"
BASE COUNT          100 a          149 c          106 g          104 t
ORIGIN      
        1 cctcacggtt cattagtacc ggttagctca acgcatcgct gcgcttacac acccggccta
       61 tcaacgtcgt cgtcttcaac gttccttcag gacccttaaa gggtcaggga gaactcatct
      121 cggggcaagt ttcgtgctta gatgctttca gcacttatct cttccgcatt tagctaccgg
      181 gcagtgccat tggcatgaca acccgaacac cagtgatgcg tccactccgg tcctctcgta
      241 ctaggagcag cccccctcag ttctccagcg cccacggcag atagggaccg aactgtctca
      301 cgacgttcta aacccagctc gcgtaccact ttaaatggcg aacagccata cccttgggac
      361 ctacttcagc cccaggatgt gatgagccga catcgaggtg ccaaacaccg ccgtcgatat
      421 gaactcttgg gcggtatcag cctgttatcc ccggagtac
//
LOCUS       DADMEQ010000350          454 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.352, whole genome shotgun sequence.
ACCESSION   DADMEQ010000350 DADMEQ010000000
VERSION     DADMEQ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.352"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..>454)
                     /locus_tag="JNZ05_004260"
     CDS             complement(<1..>454)
                     /locus_tag="JNZ05_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HAY8567194.1"
                     /translation="SLILSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYL
                     LLMFNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLAS
                     VREKLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHD"
BASE COUNT          106 a          113 c           88 g          147 t
ORIGIN      
        1 atcatgttcg aacaccactc caacatctgt ttgaacacct gttaaatttc tctcttgttt
       61 tgtttgaaca tctgcttcct ttctgcttct tctggcctga acagatgctt ttcctgcggc
      121 tgattttttg gttaattttt ctctgacaga tgccagatct tcctcaatcc gaagatgcat
      181 ccattcgtcg ccgttatcac aaaaaaactc ccgcaaggat ggttcaacat caacccatcg
      241 ctcgttagtc agacgggcaa tttttgccaa cctgtttttg ggtattggct ttcctgtttg
      301 ccagtaattg aacatcagca acaaatacgc gccgtgctcc tctgctgaca aatgcatggt
      361 gtcagccaga taatcagcta tgtacagttg catgtatggt aatgcggcca taattgcccc
      421 gtatgatgct acccggttgc ttagaataag tgag
//
LOCUS       DADMEQ010000351          441 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.353, whole genome shotgun sequence.
ACCESSION   DADMEQ010000351 DADMEQ010000000
VERSION     DADMEQ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.353"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            211..>441
                     /locus_tag="JNZ05_004261"
     CDS             211..>441
                     /locus_tag="JNZ05_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY8567195.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          139 a          105 c           62 g          135 t
ORIGIN      
        1 tcgaacctcg cttaccaacg cgttgtcagt catctccccc ctccttggtg catcgttata
       61 tccgtgatga gcgatttttg atattaatat ctttcaccat ccgctatttt caccacttca
      121 gaaaacaaac ttatttttat taaaatcaat tctttttctt tgaacattca actgcgatat
      181 ttgtaaaaac attaaacagg caacaataaa atgacattac caacaactat ttattcattc
      241 cccgcatatt taccccgttt ttcgtccacc gacaaaccag caaaattaaa atttcatcaa
      301 tacgcacgcg ctacattact gtcaaatcgt ggaagagatc ataattgcga tggcagacgt
      361 actgttgaga tccacaaact agatctctct gactggcaag cattcaataa gttagcaacg
      421 aagtgtaatg cttacgatgg a
//
LOCUS       DADMEQ010000352          433 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.354, whole genome shotgun sequence.
ACCESSION   DADMEQ010000352 DADMEQ010000000
VERSION     DADMEQ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.354"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            20..433
                     /locus_tag="JNZ05_004262"
                     /pseudo
     CDS             20..433
                     /locus_tag="JNZ05_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          121 a           98 c          118 g           96 t
ORIGIN      
        1 accagtatca cttatttaag tgatagtctt aatactagtt tttagactag tcattggaga
       61 acagatgatt gatgtcttag ggccggagaa acgcagacgg cgtaccacac aggaaaagat
      121 cgcaattgtt cagcagagct ttgaaccggg gatgacggtc tccctcgttg cccggcaaca
      181 tggtgtagca gccagccagt tatttctctg gcgtaagcaa taccaggaag gaagtcttac
      241 tgctgtggcc gccggagaac aggttgttcc tgcctctgaa cttgctgccg ccatgaagca
      301 gattaaagaa ctccagcgcc tgctcggcaa gaaaacgatg gaaaatgaac tcctcaaaga
      361 agccgttgaa tatggacggg caaaaaagtg gatagcgcac gcgcccttat tgcccgggga
      421 tggggagtaa gct
//
LOCUS       DADMEQ010000353          400 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.355, whole genome shotgun sequence.
ACCESSION   DADMEQ010000353 DADMEQ010000000
VERSION     DADMEQ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.355"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            150..335
                     /locus_tag="JNZ05_004263"
                     /pseudo
     CDS             150..335
                     /locus_tag="JNZ05_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          115 a           95 c          105 g           85 t
ORIGIN      
        1 acggtggtcg gtttacgctt accgcaggac aggtggtaac tgaaaaattg ctgaggcgac
       61 tggaaacaga agggcaagat cgattccgac agcagtgatg gtcagctacc tcatcgataa
      121 ctttgcaaca gtgcccttct tgaagcatca tgcttcatga agaaaaactg gcacgtcatc
      181 aacgtaaaca ggcgatgtat acccgaatgg tagccttccc ggcggtgaaa atgttcgaag
      241 agtatgactt cacgttcgcc accggagcac cgcagaagca gctccagtcg ttacgctcac
      301 tcagcttcat agagcgcaat gaaaacatag ttttacaggg gacatctgac atcacaaatc
      361 cccgggtggg gatttgtgta taagagacag cccatggagg
//
LOCUS       DADMEQ010000354          390 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.356, whole genome shotgun sequence.
ACCESSION   DADMEQ010000354 DADMEQ010000000
VERSION     DADMEQ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.356"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(101..>390)
                     /locus_tag="JNZ05_004264"
     CDS             complement(101..>390)
                     /locus_tag="JNZ05_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY8567196.1"
                     /translation="EDINKPISIAAANRFVNRIRGGMDLGYWRTHDFRRTLVTRLSEM
                     NVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELHADKLFWHIRSISD"
BASE COUNT           91 a           94 c           86 g          119 t
ORIGIN      
        1 ggaaaatcga tactggaaaa actctaactc ggtggccagt ttttgttgac cacttcatac
       61 cttcgtgaag ctgttgaagg gtggattctt aacggtggcg ttaatcagaa atgctcctga
      121 tgtgccagaa caatttatca gcgtgaagct catacgcttt gcgctgagcc tctatccagt
      181 cgtgtttatt gtatacggac attatcccgc caagttcatg accgagcatt cgctcagtaa
      241 catgaggctc gacattcatc tcggacagcc gtgtaacaag cgttcttctg aaatcatgtg
      301 ttcgccagta acctagatcc atccctcccc ttattctatt tacgaatcgg ttggctgcag
      361 cgatgctgat cggtttgttt atgtcttctc
//
LOCUS       DADMEQ010000355          382 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.357, whole genome shotgun sequence.
ACCESSION   DADMEQ010000355 DADMEQ010000000
VERSION     DADMEQ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.357"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            27..>382
                     /locus_tag="JNZ05_004265"
     CDS             27..>382
                     /locus_tag="JNZ05_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY8567197.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGA"
BASE COUNT          101 a           80 c          112 g           89 t
ORIGIN      
        1 tgatttaatt tatcgcgaga aaaacaatgt ctgtagtgat atcaggtgcg ctgactgatg
       61 gcgcaggtat ccccatgtcc ggataccata ttattctgaa atcccgggta aacaccccgg
      121 aagtggtgat gaacactgtt gctgatgtga tgacaggaaa cgatggtgaa tactgtttcc
      181 atgcgcggac tggaaaatat ggtgtgtatc tgaaacagga ctggcgcaac gagtacaacg
      241 ttggcgacat tgctgtatat gaggactcaa agcccggcac gctgaatgac tttctgattg
      301 ctcctgatga gggcgacctg aaaccggatg tcgtcaaacg ctttgaggaa atggtggcgc
      361 aggcgcagca gagcgccggg gc
//
LOCUS       DADMEQ010000356          376 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.358, whole genome shotgun sequence.
ACCESSION   DADMEQ010000356 DADMEQ010000000
VERSION     DADMEQ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.358"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            265..372
                     /gene="ldrD"
                     /locus_tag="JNZ05_004266"
     CDS             265..372
                     /gene="ldrD"
                     /locus_tag="JNZ05_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HAY8567198.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT          102 a           85 c          109 g           80 t
ORIGIN      
        1 taatattctg cggaatgcgc agcacagagt gaagatgaga cggattccgg aacagaaaaa
       61 cagtcgtaga atcagcgtca agccggaagg attccggtga ggcgcaatgt tgcgggggct
      121 taatccctgg tggcattggt tgctggagag agaaaacccc cgcacgttgc aggtatgcac
      181 ctgacaacac cacgggggcc attcttgcat ccaaacaata caagaatagc cgcgaaacgt
      241 tgtcattaca atacaggcgg ctatatgacg ttcgcagaac tgggcatggc cttctggcat
      301 gatttagcgg ctccggtcat tgctggtatt cttgccagta tgatcgtgaa ctggctgaac
      361 aagcggaagt aacgtg
//
LOCUS       DADMEQ010000357          375 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.359, whole genome shotgun sequence.
ACCESSION   DADMEQ010000357 DADMEQ010000000
VERSION     DADMEQ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.359"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..>375
                     /locus_tag="JNZ05_004267"
     CDS             <1..>375
                     /locus_tag="JNZ05_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAY8567199.1"
                     /translation="YREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKM
                     AEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGR
                     AFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          114 a           72 c          116 g           73 t
ORIGIN      
        1 gatacaggga gataaaacag acgatgcaac tgagcaggct gacgctgaga agtaaaaagc
       61 cggagcttgt ggagcaagag ctgtggggtg tcttactggc ttataatctg gtgagatatc
      121 agatgattaa aatggcggaa catctgaaag gttactggcc gaatcaactg agtttctcag
      181 aatcatgcgg aatggtgatg agaatgctga tgacattgca gggcgcttca ccgggacgta
      241 taccggagct gatgcgcgat cttgcaagta tgggacaact tgtgaaatta ccgacgagaa
      301 ggggaagggc cttcccgaga gtggtaaagg agaggccctg gaaatacccc acagccccga
      361 aaaagagcca gtcag
//
LOCUS       DADMEQ010000358          370 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.360, whole genome shotgun sequence.
ACCESSION   DADMEQ010000358 DADMEQ010000000
VERSION     DADMEQ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.360"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..>370
                     /locus_tag="JNZ05_004268"
     CDS             <1..>370
                     /locus_tag="JNZ05_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8567200.1"
                     /translation="PLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKT
                     AQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQ
                     MELTSHLLTAAAFGTMKNSEN"
BASE COUNT           76 a          127 c          102 g           65 t
ORIGIN      
        1 cctcttcacc agattgtgaa tcgcctggac atcatgctgc cgggcaatcg ccccttcgtt
       61 gcccccagtg ccgttattca ggcccgccag cgcctgggaa gtgaggctgt ccgccgcgtg
      121 ttcacgaaaa cagcgcagct ctggcataac gccacgccgc atccgcactg gtgcggcctg
      181 accctgctgg ccatcgatgg tgtgttctgg cgcacaccgg atacaccaga gaacgatgca
      241 gccttccccc gccagacaca tgccgggaac ccggcgctct acccgcaggt caaaatggtc
      301 tgccagatgg aactgaccag ccatctgctg acggctgcag ccttcggcac gatgaagaac
      361 agcgaaaatg
//
LOCUS       DADMEQ010000359          368 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.361, whole genome shotgun sequence.
ACCESSION   DADMEQ010000359 DADMEQ010000000
VERSION     DADMEQ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.361"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            19..368
                     /gene="tnpA"
                     /locus_tag="JNZ05_004269"
                     /pseudo
     CDS             19..368
                     /gene="tnpA"
                     /locus_tag="JNZ05_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          103 a           77 c           84 g          104 t
ORIGIN      
        1 aatcgtttca caggtcaaat gatactttgg tctgtgaaaa aagaaaccga tatccggcgt
       61 ggaaggcatt gtgttttcct gatgcatgtt cacctggtct ttgtcaccag ataccgacgc
      121 cagatttttg accatgacgc gacagaaaaa ctacgcactt acttttcaaa tgtatgtgct
      181 tattttgaag ctgaactggt tgaaatggat ggcgaaccag atcacgtcca tttgttaatt
      241 aattaacctc ccaaactggc gatatccagt ctggtaaaca gcctcaaagg cgtatcgggt
      301 aggttactgc gacgagatcg accagatatt gcagtcaggt attactacaa aggcgttttg
      361 tggagtcc
//
LOCUS       DADMEQ010000360          356 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.362, whole genome shotgun sequence.
ACCESSION   DADMEQ010000360 DADMEQ010000000
VERSION     DADMEQ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.362"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            65..140
                     /locus_tag="JNZ05_004270"
     tRNA            65..140
                     /locus_tag="JNZ05_004270"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99..101,aa:Glu,seq:ttc)
BASE COUNT           97 a           77 c           88 g           94 t
ORIGIN      
        1 agtgctcaca cagattgtct gatagaaagt gaaaagcaag gcgtcttgcg aagcagactg
       61 atacgtcccc ttcgtctaga ggcctaggac accgcccttt cacggcggta acaggggttc
      121 gaatccccta ggggacgcca cttgctggtt tgtgagtgaa agtcacctgc cttaatatct
      181 caaaactcat cttcgggtga tgtttgagat atttgctctt taaaaatctg gatcaagctg
      241 aaaattgaaa cactgaacaa tgaaagttgt tcgtgagtct ctcaaatttt cgcaactctg
      301 aagtgaaaca tcttcgggtt gtgaggttaa gcgactaagc gtacacggtg gatgcc
//
LOCUS       DADMEQ010000361          352 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.363, whole genome shotgun sequence.
ACCESSION   DADMEQ010000361 DADMEQ010000000
VERSION     DADMEQ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.363"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..230)
                     /locus_tag="JNZ05_004271"
     CDS             complement(<1..230)
                     /locus_tag="JNZ05_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY8567201.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHL"
BASE COUNT          128 a           55 c           53 g          116 t
ORIGIN      
        1 aggtgaatag tgaaaggttc agccaaagat ttctgatcgc ttttcagtac ggtatctgct
       61 acttcaccca gatttacagt taaatcttcg tcacctggtt taatagaaca tggagcagag
      121 ataacttcac ctttaaattt aacaataccg tgaccttcat cagcaaacac gttattggaa
      181 agaatcattg ctacaccagc aacaagaagt gactttttaa aagcagacat aatattcctt
      241 tatataaaga taagtaaaaa agagaatgat tagtaactgt tatttaagga ctaccaatca
      301 ttaaaatatt atattttgtt ttgtgatttt tataaaatta gtttatctaa at
//
LOCUS       DADMEQ010000362          351 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.364, whole genome shotgun sequence.
ACCESSION   DADMEQ010000362 DADMEQ010000000
VERSION     DADMEQ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.364"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..208)
                     /locus_tag="JNZ05_004272"
     CDS             complement(<1..208)
                     /locus_tag="JNZ05_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY8567202.1"
                     /translation="MSKIDYQALRAKAEKATKGSYIVGHTSVNRHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDVEVVNK"
     gene            complement(205..>351)
                     /locus_tag="JNZ05_004273"
     CDS             complement(205..>351)
                     /locus_tag="JNZ05_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HAY8567203.1"
                     /translation="PGILPAEMIIRQRVKPMPSREELLKRKSFGSVNDNKYLNAMWRK
                     GGNQ"
BASE COUNT           80 a           94 c           64 g          113 t
ORIGIN      
        1 ttttgttgac cacttcaaca tctgtttcaa ccaggcagtt aacatgacat tccgcaatca
       61 caccaccggg ttctcctttc catttttgac aaacaaaaac tcctgttaaa ttgccgtgcc
      121 gattaacaga tgtatgccct acgatgtagc ttcctttagt tgctttttct gccttggcac
      181 gcagtgcctg atagtcaatc ttgctcactg gttgcctcct ttgcgccaca tcgcattcag
      241 atatttgttg tcattaacag aaccgaaact ctttctctta agcaattcct ctctcgatgg
      301 cattggcttt acgcgctggc gaataatcat ttctgccgga agaatgccgg g
//
LOCUS       DADMEQ010000363          327 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.365, whole genome shotgun sequence.
ACCESSION   DADMEQ010000363 DADMEQ010000000
VERSION     DADMEQ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.365"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(1..327)
                     /locus_tag="JNZ05_004274"
                     /pseudo
     CDS             complement(1..327)
                     /locus_tag="JNZ05_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT           95 a           80 c           53 g           99 t
ORIGIN      
        1 caggctgtac gcttccccat gtgatttgcc tcgccacttc accgggctca ggaccccgta
       61 ggatcttcac cgaaaaaaat gcatcaccag ttgctttatt atttgtttta ggtagacctg
      121 ttactaattt atacctactc tgaccaagtc tggataagaa aggcacacct gcacatttct
      181 acttggaaat gttttctttt accattttgc tcaaaaattt caacatccaa tattcctcgt
      241 acagaactac tcaagtcctg aatagaaaaa gttccagctg gtacagtaga gtcatatatt
      301 actcgacctt gttgagatac aacaaca
//
LOCUS       DADMEQ010000364          322 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.366, whole genome shotgun sequence.
ACCESSION   DADMEQ010000364 DADMEQ010000000
VERSION     DADMEQ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.366"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..>322)
                     /locus_tag="JNZ05_004275"
     CDS             complement(<1..>322)
                     /locus_tag="JNZ05_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY8567204.1"
                     /translation="MDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLRYNSHRTK
                     REEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITETVRKTAM
                     QITWR"
BASE COUNT           73 a          101 c           61 g           87 t
ORIGIN      
        1 ctctccaggt gatttgcatc gccgtttttc tcactgtctc cgttatgatg tacaccactt
       61 cttccagtaa ccgccgtttc gccggactca aaaaaccgta gtacctcacc atacggaacc
      121 ccttatccgc cacatgccag gagaaccttt ccatgaactc atctccactc atcaacaggt
      181 attcttcccg ttttgttcgg tgactgttgt aacgcagacc gatttcatcc tgaccggcat
      241 aatgctccag acgactcatc ggcaccggtg gctttttcag gtaagagcca aagtacaccg
      301 ccacatgggt ggcattatcc at
//
LOCUS       DADMEQ010000365          314 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.367, whole genome shotgun sequence.
ACCESSION   DADMEQ010000365 DADMEQ010000000
VERSION     DADMEQ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.367"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
BASE COUNT          102 a           56 c           73 g           83 t
ORIGIN      
        1 gccagtctag cagttgacgg ggtatctgct gcgctggcca tgagaggaag agcggcggcg
       61 acagcaacaa caagacgttt gggtgaaaac gaaaattcca tgcaaaatgc tccggtttca
      121 tgtcatcaaa atgatgacgt aattaagcat tgataattga gatccctctc cctgacagga
      181 tgattacata aataatagtg acaaaaataa attatttatt tatccagaaa atgaattgga
      241 aaatcaggag agcgttttca atcctacctc tggcgcagtt gatatgtaag gcaggtttat
      301 tatatcgcgt tgat
//
LOCUS       DADMEQ010000366          313 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.368, whole genome shotgun sequence.
ACCESSION   DADMEQ010000366 DADMEQ010000000
VERSION     DADMEQ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.368"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..170
                     /locus_tag="JNZ05_004276"
     CDS             <1..170
                     /locus_tag="JNZ05_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAY8567205.1"
                     /translation="AGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRRE
                     IEREVCLWGQQ"
     gene            167..>313
                     /locus_tag="JNZ05_004277"
     CDS             167..>313
                     /locus_tag="JNZ05_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAY8567206.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANA"
BASE COUNT           85 a           75 c           86 g           67 t
ORIGIN      
        1 gcgctggcaa tttcagaaca tcgacgcttc ttcgcaaaat aaaccagggc gatatcaaag
       61 gcgcatgtga ccagctacgt cgctggacat atgctggcgg taagcaatgg aaagggctga
      121 tgacccgtcg tgagattgag cgtgaagtct gtttgtgggg gcagcaatga gcagagtcac
      181 cgcgattatc tccgctctgg ttatctgcat catcgtctgc ctgtcatggg ctgttaatca
      241 ttaccgtgat aacgccatta cctacaaaga gcagcgcgac aaaaacgcaa gagaactgaa
      301 gctggcgaac gca
//
LOCUS       DADMEQ010000367          293 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.369, whole genome shotgun sequence.
ACCESSION   DADMEQ010000367 DADMEQ010000000
VERSION     DADMEQ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.369"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            24..>293
                     /locus_tag="JNZ05_004278"
     CDS             24..>293
                     /locus_tag="JNZ05_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAY8567207.1"
                     /translation="MLTLRTSRGAYSLRKAVAVAIGGGAIAIASVLITGPGGNDGLEG
                     VSYIPYKDIVGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATV"
BASE COUNT           75 a           71 c           73 g           74 t
ORIGIN      
        1 tggaacttct cgtctgtaaa ataatcctta cgctacgtac ttctcggggc gcttactcat
       61 tacgcaaggc tgttgcagtt gctattggtg gtggggctat tgccatagcg tctgtgctca
      121 tcactggccc aggtggtaac gatggtctgg aaggtgtcag ctacatacca tacaaagata
      181 tcgttggcgt atggactgta tgccacggac acaccggaaa agacatcatg ctcggtaaaa
      241 cgtataccga agcagaatgc aaagccctcc tgaataaaga ccttgccact gtc
//
LOCUS       DADMEQ010000368          289 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.370, whole genome shotgun sequence.
ACCESSION   DADMEQ010000368 DADMEQ010000000
VERSION     DADMEQ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.370"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..>289
                     /locus_tag="JNZ05_004279"
     CDS             <1..>289
                     /locus_tag="JNZ05_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAY8567208.1"
                     /translation="TLMDKGYYSLGLLNGWSLAGEHRHWMIPLRKGAQYEELRKLGKG
                     DHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGG"
BASE COUNT           84 a           68 c           89 g           48 t
ORIGIN      
        1 gacgttaatg gataaaggtt attactcact gggactgtta aatggctgga gcctggcggg
       61 agaacaccgc cactggatga tacctctcag aaagggagcg caatatgaag agctcagaaa
      121 actgggtaaa ggcgatcatc tggtgaagct gaaaaccagc ccgcaggcac gaaaaaagtg
      181 gccgggactg ggaaatgaag tgacagcccg cctgctgacc gtgacgcgca aaggaaaagt
      241 ctgccatctg ctgacgtcga tgacggacgc catgcgcttc cccggagga
//
LOCUS       DADMEQ010000369          287 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.371, whole genome shotgun sequence.
ACCESSION   DADMEQ010000369 DADMEQ010000000
VERSION     DADMEQ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.371"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            <1..>287
                     /locus_tag="JNZ05_004280"
     CDS             <1..>287
                     /locus_tag="JNZ05_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY8567209.1"
                     /translation="LRRTDDWMDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALL
                     RRQAELDGMPAINAKRVYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESN"
BASE COUNT           70 a           72 c           82 g           63 t
ORIGIN      
        1 ttctcagacg aaccgatgac tggatggatg gccgccgcag tcgtcacact gatgatacgg
       61 atgtgcttct ccgtatacac catgttatcg gagagctgcc cacgtatggt tatcgtcggg
      121 tatgggcgct gcttcgcaga caggcagaac ttgatggtat gcctgcgatc aatgccaaac
      181 gtgtttaccg gatcatgcgc cagaatgcgc tgttgcttga gcgaaaacct gctgtaccgc
      241 catcgaaacg ggcacataca ggcagagtgg ccgtgaaaga aagcaat
//
LOCUS       DADMEQ010000370          285 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.denovo.372, whole genome shotgun sequence.
ACCESSION   DADMEQ010000370 DADMEQ010000000
VERSION     DADMEQ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.denovo.372"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(<1..>285)
                     /locus_tag="JNZ05_004281"
     CDS             complement(<1..>285)
                     /locus_tag="JNZ05_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY8567210.1"
                     /translation="AFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQ
                     SCGTWYISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSF"
BASE COUNT           68 a           75 c           73 g           69 t
ORIGIN      
        1 tgaaaactgt ttacaggctc aaagactgtg ccatctgaca gcgtggcgag tttagccacg
       61 ccagcatcca gcccgatcat tgatgctgaa gggtgaaccg gagttgatac ttcactttct
      121 gtctgaatac tgatgtacca cgtaccgcag gactggctga cagtgacatt tttcacaact
      181 cccgtgacct gacggctgtt ccggtagcgc atccagccaa gtttcggcag aaaaatacgg
      241 ctgttttcct gatcgagctt aacgccctgc gggtagcgga atgca
//
LOCUS       DADMEQ010000371        17423 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.guided.076, whole genome shotgun sequence.
ACCESSION   DADMEQ010000371 DADMEQ010000000
VERSION     DADMEQ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.guided.076"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="JNZ05_004282"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="JNZ05_004282"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAY8567211.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="JNZ05_004283"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="JNZ05_004283"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAY8567212.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="psd"
                     /locus_tag="JNZ05_004284"
     CDS             2040..3008
                     /gene="psd"
                     /locus_tag="JNZ05_004284"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAY8567213.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDARPLVDDKKDQV"
     gene            3030..6341
                     /gene="mscM"
                     /locus_tag="JNZ05_004285"
     CDS             3030..6341
                     /gene="mscM"
                     /locus_tag="JNZ05_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAY8567214.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQGLRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALRSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(6492..7994)
                     /gene="yjeM"
                     /locus_tag="JNZ05_004286"
     CDS             complement(6492..7994)
                     /gene="yjeM"
                     /locus_tag="JNZ05_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HAY8567215.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8213..9190)
                     /gene="epmA"
                     /locus_tag="JNZ05_004287"
     CDS             complement(8213..9190)
                     /gene="epmA"
                     /locus_tag="JNZ05_004287"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY8567216.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEANIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9515..11323
                     /gene="frdA"
                     /locus_tag="JNZ05_004288"
     CDS             9515..11323
                     /gene="frdA"
                     /locus_tag="JNZ05_004288"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAY8567217.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTCLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            11316..12050
                     /locus_tag="JNZ05_004289"
     CDS             11316..12050
                     /locus_tag="JNZ05_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY8567218.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12061..12456
                     /gene="frdC"
                     /locus_tag="JNZ05_004290"
     CDS             12061..12456
                     /gene="frdC"
                     /locus_tag="JNZ05_004290"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY8567219.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12467..12826
                     /gene="frdD"
                     /locus_tag="JNZ05_004291"
     CDS             12467..12826
                     /gene="frdD"
                     /locus_tag="JNZ05_004291"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY8567220.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            12889..14022
                     /gene="blaEC"
                     /locus_tag="JNZ05_004292"
     CDS             12889..14022
                     /gene="blaEC"
                     /locus_tag="JNZ05_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY8567221.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEKKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            14111..14644
                     /locus_tag="JNZ05_004293"
     CDS             14111..14644
                     /locus_tag="JNZ05_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY8567222.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14641..14870)
                     /locus_tag="JNZ05_004294"
                     /pseudo
     CDS             complement(14641..14870)
                     /locus_tag="JNZ05_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(15046..15192)
                     /gene="ecnB"
                     /locus_tag="JNZ05_004295"
     CDS             complement(15046..15192)
                     /gene="ecnB"
                     /locus_tag="JNZ05_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY8567223.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15303..15428)
                     /gene="ecnA"
                     /locus_tag="JNZ05_004296"
     CDS             complement(15303..15428)
                     /gene="ecnA"
                     /locus_tag="JNZ05_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY8567224.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(15480..16046)
                     /gene="efp"
                     /locus_tag="JNZ05_004297"
     CDS             complement(15480..16046)
                     /gene="efp"
                     /locus_tag="JNZ05_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY8567225.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16088..17116
                     /gene="epmB"
                     /locus_tag="JNZ05_004298"
     CDS             16088..17116
                     /gene="epmB"
                     /locus_tag="JNZ05_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY8567226.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         4340 a         4566 c         4486 g         4031 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc caaaaaagtt ttttttgcgt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcgat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatcc agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 agacttccag ctccggcatg tatttaaaca ggaaacgacg gtcctgtccg atgctgttac
      481 cgcaaatcgg cgattttccc gcaggcaccc actgttttaa gaattcgagc gttgccagtt
      541 cagcttcccg atcgcccatc gtgctcgctt tcacgcgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcggactga tgtactgcaa
      661 tggtcggccc ttctgccaga atattcagat tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtcagtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgctgg taaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcggtgtt aacgcgtccg gatttctacg acggcgtgaa acccatagcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat cgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gcgttcgtca acgagcagat ggatatctac cgcaacatcg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctgaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgtcggtaaa tccagcctgc
     1561 tgaacgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggctcggtca gcacaccacg accgccgctc ggctgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgttgg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt actggtcatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccgc
     2461 gtgactacca tcgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtgc
     2521 cgggcgatct cttttccgtt aaccatctca ccgctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatttgc cttttcgata ccgaatttgg cccaatggcg cagattctgg
     2641 tcggagcgac gattgttggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggtat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aactcggttc caccgttatc aacctgtttg
     2821 caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaaccc gccccacttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccaggccatt ggttgacgat aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcactcagg
     3121 aactggagca ggcaaaagcg gcgaaacccg cacagccgga agtcgtagag gcgctccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaaa caatatcagc
     3241 aagttatcga taattatccg aaactctccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgagcc gcgcagcgtg tcgccgggaa tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagccagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgac gcccgccgtc
     3481 agttaaatga gatcgagcgc cgcctgggaa cgcttaccgg caatactccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactctg cacgtcttaa ggcgctcgtt gatgaactgg
     3601 agctggcgca gctgtctgcc aataaccgcc aggaattagc gcgcttacgc tcagagctgg
     3661 cggaaaaaga gagccagcaa ctggatgcgt atttgcaggg cttacgtaat cagttgaata
     3721 gccaacgtca gcttgaggcg gagcgggcgc tggaaagtac cgaattactg gcagaaaaca
     3781 gtgccgattt gccgaaagat atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcag
     3841 cgctgaatca acaggcgcag cggatggagc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcgtccaa cctgctcggc gaagcgctgc gggcgcaggt ggcacggcta ccagaaatgc
     4021 caaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatttact taataaacag ccgctgctac ggcaaatcca tcaggccgac ggtcagccgc
     4141 tgaccgccga gcaaaaccgt attctggaag cacaactgcg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacgctac tattggaact gaccaagctg aaagtctcca
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc aacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagcaaaga gacaattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttgcgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgttaccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggcgat ggtgtaacgg ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc ccgtgggatg cgctactacc tgatgagcat cgggctgatt atggcgctga
     4861 tgatgttcga taacctcgac gaccgcgaat tttccggttc gctgggacgg ctttgcttta
     4921 tcctcatttg cggtgcgctg gcggtggtca ccctaagcct gaaaaaagcc ggtattccgt
     4981 tgtatctcaa caaagaaggc agcggcgaca acattaccaa ccatatgctg tggaacatga
     5041 tgattggcgc accactggtt gccattctgg cgtcggcggt gggttatctg gcaacggcgc
     5101 aggcactgtt agcgaggctt gaaacctcgg ttgccatctg gttcctgcta ctggtggttt
     5161 atcacgttat ccgccgctgg atgctgatcc agcgtcgcag gctggcgttt gaccgggcga
     5221 agcatcgccg ggcagagatg ttagcgcaac gtgcgcgtgg cgaagaggaa gcgcatcatc
     5281 acagtagccc ggaaggagca attgaagtcg atgaaagcga agtcgatctc gatgccatca
     5341 gtgcgcaatc cttgcggctg gtgcgctcaa ttttgatgtt gatcgccctg cgttcggtca
     5401 ttgtgctgtg gtcagaaatc cattccgctt tcggcttcct cgaaaatatt tcgctgtggg
     5461 atgtcacctc cacggtacag ggcgtagaaa gtctggagcc aattaccctc ggtgcggtgc
     5521 tgattgccat tctggtattt atcatcacca cgcagctggt gcgcaacctg cccgcgctgc
     5581 tggaactggc gattttgcag cacctggatt taacgccggg tacgggttac gccatcacca
     5641 ccatcacaaa atatctgctg atgctgattg gcgggctggt cggcttctca atgattggta
     5701 ttgagtggtc gaaattgcag tggctggttg ccgcgctcgg tgttggtctc ggttttggtt
     5761 tgcaggaaat tttcgccaac tttatctctg gcctgatcat cctgttcgaa aaaccgattc
     5821 gcattggcga tacggtaacg atccgcgatc ttactggtag cgtgacaaaa attaacaccc
     5881 gcgccaccac catcagcgac tgggaccgta aagagataat cgtgccgaac aaggcgttta
     5941 ttaccgagca gtttatcaac tggtcgctct ctgactcggt cacgcgcgta gtgttgacga
     6001 ttccggcccc tgccgatgcc aacagtgaag aagtgacgga aatcctgctc accgcagcgc
     6061 gtcgctgctc gctggtgatc gacaacccgg caccggaagt cttcctggtg gatctgcaac
     6121 aggggattca gattttcgag ctgcgtattt acgccgctga gatgggtcac cgtatgccgc
     6181 tacgccatga gatccaccag ctgattctgg caggcttcca cgcccacggt atcgatatgc
     6241 cattcccgcc cttccagatg cgtctggaaa gcctcaacgg taaacaaacg gggagaacgc
     6301 tgacgtctgc gggcaaaggt cgtcaggcgg gaagtttgta atgcatgaag ctgccaaatg
     6361 atgtcaacgt ggctcattta gcagcttaac atcatcttca gttatcaccg gccctggaga
     6421 gtatcatcca tacgcattac ttcctgacaa ctcatcgccg gagagacgat gagttgcagg
     6481 atttcatatg atcagtcgag agctaactca ggtttattcg ccatgcgact gcaatagttc
     6541 tggtaaatcg ccatcgctaa cagcgagaag aagacagggc cgccaatcat ccacaatgtg
     6601 ctgtcccagt ctccggcttc aaccacaggc tgaataatgg tgaagacgtt cgcaaatgtc
     6661 accaccagaa cgacaaccac cgtcgcaatc attgccgaca tacgcgtttt gaaaatcaca
     6721 aatggtctgt cgagatcctg acgtgcttta aaaaacggga aagccagcgc gaggaacagg
     6781 taaggaagcg tcatagacac gttcgccatc agcgtcagct tgttaaagaa cgccgatgcg
     6841 gtaccgccac caaacgaaac cagcagaatg aagacagtaa ccaacccgca ctgcatccac
     6901 atggcgatag acggcatccc catcgcattc aggcgcgtca tcggttccgg ccacaacgct
     6961 ttcggcgtcc cctggatgat ggctttcagc ggtgaatagc aaagcgtaaa gaacgcaccg
     7021 gtataagcga ggaacatcga aagtccggta atacgcgcaa accatacgcc cagcgacaat
     7081 gacgcttcag gtgacaaatg cagtgcgtta cccagcgtca ccccgaggct cttcatcagc
     7141 acataggtaa tattgccaag gttaacggaa ccattactta atacctgctg ccagtttgtg
     7201 ctgacgcccc ataaaaatat tgccagcgaa taaccgattg aaataacaat agcggcaaaa
     7261 acaatacctt tggcaaagtt cttttctgga ttttccgttt tatcgaccag accaccaacc
     7321 gcttcaattc cgccataggc aaaaatagca aataccacaa acgatagcat tgccagaccg
     7381 gactgataac ccgggttcgg tgatgcaagg aaattaatat cctgcgcgaa atgcccacca
     7441 tttaataaca aaatagtaat gcttactaac agcaatacta aattcagaca cattactgca
     7501 ataccgccca ccgcagtaat gcgggcaatt ttattaatcc cctttgaagc aacgacggtg
     7561 accagaatca tccatgctac tgccagcaga ccaaccacct gcgtaggctc cagtccggca
     7621 atacgccagt gctgggtcat gtcactacca tagaggaatg ttgagaacgg tacccaaact
     7681 ttcgcggagg tactcaccat ccagatgata taagaggaaa accacataaa cgtaccaatg
     7741 aaggcaaaac gtggtccgac actattattc atccaggaat agataccacc ttcttctttg
     7801 cgataagcag ctcccatttc agccatcatt aaggcgaatg gaataaagaa taacaatgca
     7861 gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga tgggctattg
     7921 gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact catctttttt
     7981 atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt gcccactgaa
     8041 ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga taccttgatg
     8101 atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac atatgctaca
     8161 gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag aattatgccc
     8221 ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc aacatcacca
     8281 gacgatcaac acctaatgcc acgccggagc agtcaggcat accgactttc aaggcttcaa
     8341 tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta cggttatctt
     8401 gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca ttcgccagct
     8461 caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg gtactgattt
     8521 gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt tctttaccaa
     8581 tatttgcctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg tcttcttcgg
     8641 tatcagcaac attgctcaaa tccagtttcg ctgcgacttc ccgcagttgc gttttgtcgg
     8701 cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa aggctttctg
     8761 ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc aaccggtaca
     8821 tatcatagtg cggtcgatac cactccagca tagtgaactc agggttgtga taacgcccca
     8881 tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaacca gcaaccagca
     8941 ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc tgcgaatgcc
     9001 cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc gtcgcctggc
     9061 tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga cggatctccg
     9121 ccataatcgc cgcgcgtttt aataagttag gaatggatgc gctcggctgc caggatgccg
     9181 tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact cgcaacgcga
     9241 cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa aaaaagcacg
     9301 atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc tcttgcgcac
     9361 taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg ttgtacctat
     9421 aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc ctgaagtacg
     9481 gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag ccgatcttgc
     9541 cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc aggcaaatcc
     9601 aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata ccgttgctgc
     9661 agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc actttcacga
     9721 tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt tcgtccacca
     9781 ctgcccaacc gaaatgaccc aactggaact gtggggatgc ccatggagcc gtcgcccgga
     9841 tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct ggttcgccgc
     9901 cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc aattcccgca
     9961 gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg gtcatgttcg
    10021 cggcctggta gcaatgaaca tgatggaagg cacgttggtg cagatccgtg ctaacgcggt
    10081 cgttatggct accggcggtg cgggtcgcgt ttatcgttac aacaccaacg gcggcatcgt
    10141 taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg acatggaatt
    10201 cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg aaggctgccg
    10261 tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag attacggcat
    10321 gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg gtccacgcga
    10381 caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatct ccacgccgcg
    10441 tggtgatgtg gtttatctcg acctgcgtca cctcggcgag aaaaaactgc atgaacgtct
    10501 gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta aagaaccgat
    10561 tccggtacgt ccgactgcac actacaccat gggcggtatc gaaaccgatc agaactgtga
    10621 aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc tgcacggtgc
    10681 aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc tggccggtga
    10741 acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa ttgaagcgca
    10801 ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg gcgaaaactg
    10861 ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta tctaccgtac
    10921 gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac gcttcaagcg
    10981 cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca ccattgaact
    11041 gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac gtaaagagtc
    11101 ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg tcaactttct
    11161 caaacacacc ctcgccttcc gcgatgctga tggcacgact tgcctggagt acagcgacgt
    11221 gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg atgcagccga
    11281 taaggcggaa gcagccaata ataaggagaa ggcgaatggc tgagatgaaa aacctgaaaa
    11341 ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca ttctatgaag
    11401 tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa gacaacctgg
    11461 caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc tgcggcatga
    11521 tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac accgacggta
    11581 tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc gatatgaccc
    11641 acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc cgcaccgcgg
    11701 atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag ttctccggtt
    11761 gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac ccagagttca
    11821 tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc gaccacggta
    11881 agaaggagcg tatggcgcag ttgaacagcc agaacggtgt atggagctgt actttcgtgg
    11941 gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt cagcagggca
    12001 aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa ggagtgcaac
    12061 atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg gaaaaaattg
    12121 ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt gtggttcagc
    12181 attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg ggcgggattc
    12241 gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct gacggcagct
    12301 ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat cattgtaaaa
    12361 gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac tgtggttgcc
    12421 accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga ttaatccaaa
    12481 tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg gtatgtggag
    12541 cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac tggggctgtt
    12601 tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct tcattggtcg
    12661 cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc gtatgcacca
    12721 cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa tgggttttct acggtctggc
    12781 tgctatcctg acagttgtca cgctgattgg tgtcgttaca atctaacgca tcgccaatgt
    12841 aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat gttcaaaacg
    12901 acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc tcaacaaatc
    12961 aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat ccccggtatg
    13021 gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta tgcggacatc
    13081 gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt cagcaaaaca
    13141 tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt aagcgatccc
    13201 gcaacaaaat actggcctga acttaccgct aaacagtgga atgggatcac actattacat
    13261 cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt gaaatcctca
    13321 agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg aacacaacgt
    13381 ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc gtctggtttg
    13441 agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa ccatacgtgg
    13501 attaatgtac ctcccccaga aaaaaagaat tacgcctggg gatatcgcga aggtaaggca
    13561 gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc gaccattgaa
    13621 gatatggccc gctgggtgca aagcaattta cccccccttg atatcaatga gaaaacgctt
    13681 caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat gtatcagggc
    13741 ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa cggcagtgac
    13801 aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac tcctgcagta
    13861 cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta tgtcgcgttt
    13921 attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc caatccagag
    13981 agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat cgggtccgaa
    14041 ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa aaaccagtaa
    14101 ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat ttctggtcgt
    14161 tgcctgtagt tctcctacgc cgccgcgtgg cgtgaccgta gtgaataatt tcgacgccaa
    14221 acgttatctc ggtacctggt atgagattgc ccgttttgat caccgttttg aacgtggact
    14281 ggaaaaagtc accgcaacat acagcctgcg tgatgacggc ggcctgaatg tcattaataa
    14341 aggctataac cctgacagag gaatgtggca gcagagtgaa gggaaagcgt actttaccgg
    14401 cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt cctttctatg gcggttataa
    14461 cgttattgca ctcgatcggg aataccgcca tgcgctggtt tgcggcccgg accgcgacta
    14521 cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg agatgctggc
    14581 agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc agcccggtag
    14641 ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca gactcgccag
    14701 acgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg cgccgactgc
    14761 gccaataccc gtccacacgg catcaaacgg ctaaagccgt gggtatattt caggccaacg
    14821 gcccatacca cttccagcag accagcaata actaagataa tctaggacat atcaggctcc
    14881 ggaaatttgg ggccgtcccc ggtgaaaaga ggcgtttgca ggtcgtcctg caaagctgat
    14941 ggttgaacgg atattttgcc aggtggcagg ggaatttcaa ctattacctt tatgacgatc
    15001 gcagaaaaaa caaacggcac gacactgctg tcgtaccgtt attgcttatt gctgcgcttt
    15061 cgttgctgcg ccagaaatcg cgttaccgcc atcagaaatg tcttcaccaa cgccacgcgt
    15121 ggtgttgcag gcagttaata ctgttgaaag caccagaaca gaaaagatcg ctgcaattgt
    15181 cttcttcacc ataacgtctt ccttttaggt aatgttattt atgtttgcct atagcaaatt
    15241 aataatagac aacatcgcca aaaatgatgg aaatctgatg atttttcgga agagaagaaa
    15301 aattagctgg cagcgcgaga gatggagttg ccgagatgtt tgatgtcttc gccgaaaccg
    15361 caagcggtgt tacagcccgt gagcagcgtg ctggcaagca aaacaagaac gataaggcgt
    15421 ttcatcatct ctgacctgaa taagtgatgg tgcagcctgc aggccgcacc acaaccgcat
    15481 tacttcacgc gagagacgta ttcaccagag cgggtatcca ctttgatgac ttcgccgatt
    15541 tgtacgaaca gcggaacttt aaccacagcg ccagtagaca gggtagccgg tttgccgcca
    15601 gtacctgcgg tatcaccttt caggcccgga tcggtatcaa cgatttccag ttcaacgaag
    15661 ttcggcggag taacggagat cggctgacca ttccacagag ttacgataca ctctgcctga
    15721 tccagcagcc atttagcgtt gtcgccaatt gcttttgcat cagcagacag ctgctcgaaa
    15781 gtttcgttgt tcatgaagtg ccagaactca ccgtcgttgt acaggtaagt caggttcata
    15841 tcgacaacat cagcgccttc agcggaatca gtagatttga aggttttttc tacgcgagta
    15901 ccggtcagca gacgacgcag tttaacgcga gcaaatgcct ggcctttacc cggttttacg
    15961 aattcactcg cttcaaccgc gtaaggttcg ccgtctaaca tgattttaag accagcacga
    16021 aaatcgttgc tatagtacgt tgccataagg ccctctgaaa tttgttaatt ggtagctaag
    16081 ccacaaaatg gcgcatattg taaccctaaa taccccatcc agagaagatt ggttaacgca
    16141 acttgccgat gttgtgaccg atcctgatga acttctgcgt cttttgaata tagacgcgga
    16201 cgaaaaactg ttggccggac gcagcgccaa aaagctgttt gccctgcgtg tgccccgctc
    16261 atttatcgat cgcatggaga aaggcaatcc ggacgatcct cttttgcgtc aggtacttac
    16321 ctcgcaagat gagtttgtcg tcgcgtccgg attctccacc gacccactgg aagaacagca
    16381 cagcgtagtg cctggtttgt tgcataaata ccacaaccgg gcgcttttgc tggtcaaagg
    16441 cggctgcgcg gtaaattgcc gctattgctt ccgtcgccac ttcccctatg ccgaaaatca
    16501 gggcaacaag cgtaactggc agactgcact tgagtatgtt gctgcgcatc cggaactgga
    16561 cgagatgatt ttctccggcg gcgatccgct gatggcgaaa gatcacgagc tggactggtt
    16621 gctcacacaa ctggaagcca tcccgcatat caaacgtctg cggattcaca gccgtctgcc
    16681 gattgtgatc ccggcacgta tcaccgaggc gctggttgaa cgctttgccc gttctacgct
    16741 gcaaatcttg ctggtgaatc acatcaatca tgccaatgag gtagatgaaa cgttccgtca
    16801 ggcgatggct aaattgcgcc gtgtcggtgt caccctgctt aaccagagcg ttctgttacg
    16861 tggtgtgaac gataacgcac aaacgttggc aaacctgagt aatgcgttgt tcgatgccgg
    16921 cgtgatgccg tattacctgc atgtgctaga taaagtacag ggcgcggcgc attttatggt
    16981 gagtgatgac gaagcacggc agattatgcg tgagttgctg acattggtgt cgggatatct
    17041 ggtgccgaaa ctggcgcgag aaattggcgg cgaacccagc aaaacgccgc tggatctcca
    17101 gctacgccag cagtaactga ttaaacaggg aagttaaaca ttaaaattgc ctgatgcgct
    17161 acgcttatca ggcctacatc gtgcattgaa attgaatgga tttgcaatga actgtaggcc
    17221 ggataaggcg tttacgccgc atccggcaag agcaacaaac gcaagtacag caacctgtca
    17281 ttaaactgag aaatttaatc tttgcacgtt gcataacctt taattattca tatgcattgc
    17341 tgttgttact ttttatatag ctaacaccaa taattaacct ttcttttcgg taatgactcc
    17401 aacttactga tagtgtttta tgt
//
LOCUS       DADMEQ010000372         9742 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain ERS180112
            SAMEA1710176-rid11411363.guided.133, whole genome shotgun sequence.
ACCESSION   DADMEQ010000372 DADMEQ010000000
VERSION     DADMEQ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1710176
            Sequence Read Archive: ERR230542
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 26-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000941202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000941202.1
            Genome Coverage       :: 64x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/27/2021 03:46:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,308
            CDSs (total)                      :: 4,232
            Genes (coding)                    :: 3,854
            CDSs (with protein)               :: 3,854
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 378
            CDSs (without protein)            :: 378
            Pseudo Genes (ambiguous residues) :: 0 of 378
            Pseudo Genes (frameshifted)       :: 183 of 378
            Pseudo Genes (incomplete)         :: 87 of 378
            Pseudo Genes (internal stop)      :: 144 of 378
            Pseudo Genes (multiple problems)  :: 34 of 378
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9742
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1710176-rid11411363.guided.133"
                     /strain="ERS180112"
                     /serovar="3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="United Kingdom"
                     /collection_date="2009"
     gene            59..1103
                     /locus_tag="JNZ05_004299"
                     /pseudo
     CDS             59..1103
                     /locus_tag="JNZ05_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1219..1392
                     /locus_tag="JNZ05_004300"
     CDS             1219..1392
                     /locus_tag="JNZ05_004300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY8567227.1"
                     /translation="MKKALDRLIKEAARQDVAVTVLETETGRYSDTLRSALISLDGDN
                     AWALSESWCGTIQ"
     gene            1447..1779
                     /locus_tag="JNZ05_004301"
                     /pseudo
     CDS             1447..1779
                     /locus_tag="JNZ05_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
     gene            complement(1836..3293)
                     /gene="pepD"
                     /locus_tag="JNZ05_004302"
     CDS             complement(1836..3293)
                     /gene="pepD"
                     /locus_tag="JNZ05_004302"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY8567228.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3555..4013
                     /gene="gpt"
                     /locus_tag="JNZ05_004303"
     CDS             3555..4013
                     /gene="gpt"
                     /locus_tag="JNZ05_004303"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY8567229.1"
                     /translation="MSEKYIVTWDRLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4105..5349
                     /gene="frsA"
                     /locus_tag="JNZ05_004304"
     CDS             4105..5349
                     /gene="frsA"
                     /locus_tag="JNZ05_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY8567230.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5407..5808
                     /locus_tag="JNZ05_004305"
     CDS             5407..5808
                     /locus_tag="JNZ05_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY8567231.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5847..6902)
                     /gene="phoE"
                     /locus_tag="JNZ05_004306"
     CDS             complement(5847..6902)
                     /gene="phoE"
                     /locus_tag="JNZ05_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY8567232.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            7190..8293
                     /gene="proB"
                     /locus_tag="JNZ05_004307"
     CDS             7190..8293
                     /gene="proB"
                     /locus_tag="JNZ05_004307"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY8567233.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8305..9558
                     /gene="proA"
                     /locus_tag="JNZ05_004308"
     CDS             8305..9558
                     /gene="proA"
                     /locus_tag="JNZ05_004308"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY8567234.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT         2355 a         2416 c         2586 g         2385 t
ORIGIN      
        1 aattcaagtc gcagcacatg caacttgaag tatgacgagt ctatttacgg aatggtatat
       61 gggcaattat attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg
      121 gatcgagagt ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg
      181 cgtagtgggg atgccagatt tacaccccgg acgcggctac ccgattggcg cagcgttctt
      241 ctccgcaggc cgtttttacc cagcactggt cggcaatgat atcggctgtg gcatggcgct
      301 atggcaaaca gatattctcg ctcgcaaata caacgccgat aagtttgaaa agcgattatc
      361 agctttggat gacgttgctg aagaaagctg gctggaggaa aacctgccgt cagcatttgc
      421 acagcatccg tggcgcagct cgcttggctc tatcggtggt ggcaatcatt tcgctgaact
      481 gcaacaagtt gatcaaatca tcaacgctga actgtttgca ctggcaggtc tggatgcgca
      541 gcatctgcaa ctgctggtcc atagcggctc gagggagcta gggcagtcta ttttacagcg
      601 gcatattgcc tcgttttcgc atcatggttt gcctgaaggc agtgacgacg cgctggcgtt
      661 tgcgcgtttt aatcgccacc tgatcgcttt gcgcataatg caacaggtta aggccaccgg
      721 aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcgaatcg gtgatcaaca
      781 aggggtctta catcgtaaag gtgccacgcc tgatgactgt ggtctggtgg ttattcccgg
      841 ttcacgcggt gattactcca ggctggttca ggcccgtcag gagtgaggaa acattgcatt
      901 cgctggcgca tggggctggg cgtaaatggg ggcgcaccga gtgtaaaggg cgtctggcag
      961 cgaaatacac agcgacgcaa ctctcgcgta ctgaacttgg cagtcgggta atttgtcgcg
     1021 ataaacaact catcttcgaa gaagcgccac aagcctataa atcggctgaa agtgtggtgc
     1081 aatgtctggg caggccggat taattattcc tgtcgcgcgg ctgcgtccgg tgctaacgct
     1141 caaaaacagt ggagggaaaa aaggatgatc ttgctataac tctcctcagc gcaggggccg
     1201 gaagaatgtt gtctcgcagt gaaaaaggcg ctggacaggc taattaaaga agctgcccga
     1261 caagacgtcg cggtaacggt gctggaaaca gaaacaggcc gctactctga cacgctgcgt
     1321 tcggcgctga tttctctgga tggcgacaac gcatgggcgt taagcgaaag ctggtgtggc
     1381 actattcagt agatttgccc gagcccgtat cgcccacatc acgggcgtaa aaactggttt
     1441 ctgggcattg ggcgttttac cgctgatgag caggagcaat ccgatgcgat ccggtatgag
     1501 acgctgcgtt cgtcggggcc gggcggtcaa catgtcaata aaaccgactc ggcggtacgc
     1561 gccacgcatt tggcatccgg tattagcgtg aaggttcagt cagagcgcag ccagcatgct
     1621 aacaaacggc tggcgcgatt gctgattgcc aggaagctgg atcagcagca ataggaaaat
     1681 agcgcgtagc tgaaatcaca gcggcgaatg ttccatcacc agattgaacg tggcaacccg
     1741 cgacgaacgt ttacagggat ggcttttatc gaaggataat gaaggatgaa actgccggaa
     1801 ggcgattaaa cgccatccgg cagcaaatca aataattact tcgccggaat ttctttcagc
     1861 agttcagtca gcagtgtcca gtaatgacct acgctttcga tgtgaacttg ctcatccgga
     1921 gagtgtggac cggtgatagt tggcccgata gaaaccatgt ccatttccgg atacggtttt
     1981 ttgaacagac cacattccag gcccgcgtgg ataatctgga tgttcggcgt cttgttgaac
     2041 aggcgctgat aggtttcacg taccagatgc atcaccggag aattagcgtc cggctgccag
     2101 ccaggatatg cgcctttcgc ttcggttttc gcgccagcca gtttacccag cgaatccagc
     2161 atgctcacca cgtagtcttt accgctgtcg atcagtgaac ggatcaggca gtgaatttct
     2221 acgttattgt cagtcatggt caccacaccg acgttcaggg aggtttcgac cacacctttt
     2281 gccacgtcgg aattgcggat cacaccgttc ggggtggcgt tcagcagacg aataaaggta
     2341 tcgcgagatt tcgcaatcag ggcagcttta tcgttcgcta cagagtccag caacaaggcc
     2401 agatttttct ctttctctgc cagctcgttt ttcaggatct cctgataggt attcaccaga
     2461 gatttcagtg cgtcgacttt atcagctgtg acagcaatgg tcgcaaaggc ttcacgcggg
     2521 atggcgttac gcagtgtgcc gccgttgaaa tcgataaggc gcaggtccag ttcttctgca
     2581 tgacccgcca ggaagcgcac cagcagtttg ttggcattac ccaggccaac gtggatttca
     2641 ccgccggagt gaccgccttt cagacctttt aaggttaact tgaaggtttc aaaaccagct
     2701 ggaaccgctt cacgatctaa atgcaggttg gaggtgaagt cgataccccc cgcacaaccc
     2761 atgtagattt caccttcttc ttcggagtcg gtgttaatca gaatatcagc ctgcaaccag
     2821 ttgctctgta agccgaacgc accgtccata ccggcttctt cggtcatggt cagcagcact
     2881 tccagcgggc cgtgaaccac gttttcgtca gccagaaccg ccagtgcaga ggccatacca
     2941 atgccgttat ccgcacccag cgtggtgccg cgcgctttaa cccattcgcc atcaatataa
     3001 ggctggatag gatctttcgt gaagtcatgc acggtgtcgt tatttttctg cggcaccata
     3061 tcgaggtggg cctgtaagac gaccggttta cgattttcca tacctgcggt agcaggttta
     3121 cgaatcagga tattacctac ctgatcgcgt tcgacatgga aacctttctc ttttgcccaa
     3181 ccaacaatgt attcagcgag ttgctcttca tgataggacg ggtgaggaat agaacagatt
     3241 ttggcaaaaa tatcccacag cggctgtgga gataattgag acagttcaga cacgttaagt
     3301 ctccttgtcg atcacccgca aaacagtatt gcaggtcaca gggttagcag aaaatgttgt
     3361 caacacaaga caggcttgcg agatatgtat tgagaatacc actttatccc gcgtcaggga
     3421 gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagata
     3481 tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg
     3541 ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacaggttg cagatccatg
     3601 cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa
     3661 gccgtggcgg tctggtaccg ggggcgttac tggcgcgtga actgggtatt cgtcatgtcg
     3721 ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac
     3781 gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta
     3841 ctgctgttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac
     3901 cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggatcg
     3961 aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc
     4021 aacgcctggc attgccgggc gttgttcttt ttaacttcag gcaggttaca atagtttcca
     4081 gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa
     4141 ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca cggtgcacaa
     4201 ccgcctgtgc agtcggctct tgatggtaaa accatccctc actggtatcg catgattaac
     4261 cgtctgatgt ggatctggcg cggtattgac ccacgtgaaa tcctcgacgt ccaggcacgt
     4321 attgtgatga gcgatgctga acgtaccgac gatgatttat acgataccgt gattggctac
     4381 cgtggtggca actggattta tgagtgggcc acccaggcga tggtgtggca acaaaaagcc
     4441 tgtacggaag aagatccgca actcagtggt cgacactggc tgcatgcggc tacgttgtac
     4501 aacattgccg cctatcctca tctgaaagga gatgacctgg ccgagcaagc gcaggcttta
     4561 tcaaaccgcg cctatgaaga ggccgcccag cgtctgccgg gcacgatgcg gcagatggag
     4621 tttaccgtac ccggcggtgc gcccatcacc ggctttttgc atatgccgaa aggcgatggc
     4681 ccgttcccga cagtattaat gtgtggtggt ctggatgcga tgcagacgga ctattacagc
     4741 ctgtatgaac gttattttgc gccgcgcggc attgcgatgc tgactattga tatgccgtcg
     4801 gtgggctttt cttcaaaatg gaagctcacc caggactcca gcctgttgca tcagcacgtc
     4861 ttaaaggcgc tgcctaacgt accgtgggtg gatcacactc gcgtcgcggc ctttggtttc
     4921 cgtttcggcg ctaacgttgc cgtgcgtttg gcataccttg aatcgccgcg tctgaaagcg
     4981 gttgcctgtc ttggtccggt agttcatacc ctgttgagtg attttaagtg ccagcaacag
     5041 gtgccggaaa tgtatcttga cgttctggcg agtcgtttgg ggatgcatga tgcttccgac
     5101 gaagcgttac gcgtggagct gaatcgctat tcattaaaag tgcaaggatt gctggggcgt
     5161 cgctgcccaa cgccaatgtt atcaggctac tggaagaacg atccgttcag cccggaagag
     5221 gactcacgct taatcacctc atcatctgct gacggtaaat tattagagat cccatttaac
     5281 ccggtgtatc ggaattttga caaaggtctt caggaaatca ccggctggat cgaaaaacgc
     5341 ttgtgttaaa aatttgctaa attttgccaa tttggtaaaa cagttgcatc acaacaggag
     5401 atagcaatga cgttaccgag tggacacccg aagagcagat tgatcaaaaa atttaccgca
     5461 ctaggcccgt atattcgtga aggtaagtgc gaagataatc gattcttttt cgattgtctg
     5521 gctgtatgcg tcaacgtgaa accggcaccg gaagtgcgtg aattctgggg ctggtggatg
     5581 gagctggaag cgcaggaatc ccgttttacc tacagctacc agtttggtct gttcgataaa
     5641 gcaggcgact ggaagagtgt tccggtaaaa gatactgaag tggttgaacg actggagcac
     5701 accctgcgtg agtttcacga gaagctgcgt gaactgctga cgacgctgaa tctgaagctg
     5761 gaaccggcgg atgattttcg tgatgaaccg gtgaagttaa cggcgtgagt gaaatgtgcc
     5821 ggatgcatca catccggcaa tattcattaa aactgatacg tcatcccaac cgcgacagta
     5881 tcatcattat taatattcag cttattatcg ctatccagtt tgttgatttt ataatcaaca
     5941 aacgctgaca tatttttgtt gaaataatac gtagcaccga cgtcgatata attgaccaga
     6001 tcttcatcac cgatgccctc aatatctttc cctttcgata agacataacc cagcgatgga
     6061 cgcagaccaa agtcaaactg gtattgagcg accgcttcaa agttctgtgt cttattggca
     6121 aagccgccag ttattggcgt cattttgcgt gtttcagaat agaaagttgc cagataaata
     6181 ttattggcat cgtatttcag acctgttgcc catgcttctg cacgcttgcc tgtgccacgg
     6241 ctttgcaggt tctgctcgtt ggtgcgatct gagttggtat aggccccact aatggcgaaa
     6301 tcgctgccgc caaagtcata tgtcaatgac gtgccgaagc catcgccgtt ttgcttttta
     6361 acgtcgcggt tttcgttttt cccttgatat tgcagggtta agttcaggcc atcgataacg
     6421 ccgaagaagt cggtgttccg atacgtcgcc agaccgctgg cgcgtttggt cataaagttg
     6481 tcggtctgcg ccgaggaatc gccaccaaat tccgggaaca tatcggtcca ggcttccacg
     6541 tcatacaacg cgcccaggtt acgaccataa tcgaaagaac ccaaatcttt atatttcaac
     6601 ccggcaaaag cgagacgcgt tttttgctgt gcagtatcac tctccgcttt atttccggca
     6661 aactccgctt cccaccgacc ataaccagtc agttgatcgt taatttgagt ttcaccttta
     6721 aaaccaaaac ggatataact ctggtcgcca tctttactgg cgttatcact catataatgc
     6781 atggctttaa ctttgccata gacatccagt ttattaccgt ctttattata tatttctgca
     6841 gcctgtacag atgcagatgc cacaatgccc attaccacta atgccagagt gctctttttc
     6901 attttcattc ctgattttaa ttaacgcgcg aatattcagc gggagagccc cgttgaaaac
     6961 aggaaagttt ttaacctgag attgttaaag atatattaca gattaatgat attctgaaaa
     7021 tgtggtaatt tattaaatct gtaataaaag cgtaaacaac tgccgctacg cttgctgatc
     7081 ccgcgcaaca aaacgccatg ctttgctcgc agatggttgg caaccgacga cagtcctgct
     7141 aaaacgttcg tttgatatca tttttcctaa aattgaatgg cagagaatca tgagtgacag
     7201 ccagacgctg gtggtaaaac tcggcaccag tgtgctaaca ggcggatcgc gccgtctgaa
     7261 ccgtgcccat atcgttgaac ttgttcgcca gtgcgcgcag ttacatgccg ccgggcatcg
     7321 gattgttatt gtgacgtcgg gcgcgatcgc cgccggacgt gagcacctgg gttacccgga
     7381 attgccagcg actatcgcct cgaaacaact gctggcggcg gtagggcaga gtcgactgat
     7441 tcaactgtgg gaacagctgt tttcgattta tggcattcac gtcgggcaaa tgctgctgac
     7501 ccgtgctgat atggaagacc gtgaacgctt cctgaacgcc cgcgacaccc tgcgtgcgtt
     7561 gctcgatcac aatatcgttc cggtaatcaa tgagaacgat gctgtcgcta cggcagagat
     7621 taaggtcggc gataacgata acctttctgc gctggcggcg attctggcgg gtgccgataa
     7681 actgttgctg ctgaccgatc aaaaaggttt gtataccgct gatccgcgca gcaatccgca
     7741 ggcagaactg attaaagatg tttacggcat tgatgacgca ctgcgcgcga ttgccggtga
     7801 cagcgtttca ggcctcggaa ctggcggcat gagtactaaa ttgcaggccg ctgacgtggc
     7861 ttgccgtgcg ggtatcgaca ccattattgc cgcgggcagc aagccgggcg ttattggtga
     7921 tgtgatggaa ggcatttccg tcggtacgct gttccatgcc caggcgactc cgcttgaaaa
     7981 ccgtaaacgc tggattttcg gtgcgccgcc ggcgggtgaa atcacggtag atgaaggggc
     8041 aactgccgcc attctggaac gcggcagctc cctgttgccg aaaggcatta aaagcgtgac
     8101 tggcaatttc tcgcgtggtg aagtcatccg catttgcaac ctcgaaggtc gcgatatcgc
     8161 ccacggtgtc agtcgttaca acagcgatgc attacgccgt attgccggac accactcgca
     8221 agaaattgat gcaatactgg gatatgaata cggcccggtt gccgttcacc gtgatgacat
     8281 gattacccgt taaggagctg gccgatgctg gaacaaatgg gcattgccgc gaagcaagcc
     8341 tcgtataaat tagcgcaact ctccagccgc gaaaaaaatc gcgtgctgga aaaaatcgcc
     8401 gatgaactgg aagcacaaag cgaaatcatc ctcaacgcta acgcccagga tgttgctgac
     8461 gcgcgagcca atggccttag cgaagcgatg cttgaccgtc tggcactgac gcccgcacgg
     8521 ctgaaaggca ttgccgacga tgtacgtcag gtgtgcaacc tcgccgatcc ggtggggcag
     8581 gtaatcgatg gcggcgtact ggacagcggc ctgcgtcttg agcgtcgtcg cgtaccgctg
     8641 ggggttattg gcgtgattta tgaagcgcgc ccgaacgtga cggttgatgt cgcttcgctg
     8701 tgcctgaaaa ccggtaatgc ggtgatcctg cgtggtggta aagaaacctg tcgcactaac
     8761 gcggcaacgg tggcggtgat tcaggacgcc ctgaaatcct gtggcttacc ggcgggtgcc
     8821 gtgcaggcga ttgataatcc tgaccgtgcg ctggtcagtg aaatgctgcg tatggataaa
     8881 tacatcgaca tgctgatccc gcgtggtggc gctggtttgc ataaactgtg ccgtgaacag
     8941 tcgacaatcc cggtgatcac aggtggtata ggcgtatgcc atatttacgt tgatgaaagc
     9001 gcagagatcg ctgaagcatt aaaagtgatc gtcaacgcga aaactcagcg tccgagcaca
     9061 tgtaatacgg ttgaaacgtt gctggtgaat aaaaacatcg ccgatagctt cctgcccgca
     9121 ttaagcaaac aaatggcgga aagcggcgtg acattacacg cagatgcagc tgcgctgaca
     9181 cagttgcagg caggccctgc gaaggtggtg gcggttaaag ccgaagagta tgacgatgag
     9241 tttctgtcat tagatttgaa cgtcaaaatc gtcagcgatc ttgacgatgc catcgcccat
     9301 attcgtgaac acggcacaca acactccgat gcgatcctga cccgcgatat gcgcaacgcc
     9361 cagcgttttg ttaacgaagt ggattcgtcc gctgtttacg ttaacgcctc tacgcgtttt
     9421 accgacggcg gccagtttgg tctgggtgcg gaagtggcgg taagcacaca aaaactccac
     9481 gcgcgtggcc caatggggct ggaagcactg accacttaca agtggatcgg cattggtgat
     9541 tacaccattc gtgcgtaaat aaaaccgggt gatgcaaaag tagccatttg attcacaagg
     9601 ccattgacgc atcgcccggt tagttttaac cttgtccacc gtgattcacg ttcgtgaaca
     9661 tgtcctttca gggccgatat agctcagttg gtagagcagc gcattcgtaa tgcgaaggtc
     9721 gtaggttcga ctcctattat cg
//
